LOCUS       DDPQ01000001            4746 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5403,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000001 DDPQ01000000
VERSION     DDPQ01000001.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5403"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    279..391
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1249..1371
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3397..3425
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3945..4007
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4325..4441
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          983 a         1090 c         1070 g         1158 t
ORIGIN      
        1 gctgggtcgg tattaaggcc gtacttcacg cccatgacaa aagtgtagag cccaggacca
       61 tcaactgtgc cgtctgcaat cagatctttg gcaagattta gatctccgct atcaaagatc
      121 tcaagctctg gcatcacccc tgcctcacga ataacctttg ccatcttgcg gacgttactg
      181 ggggtgttca tcaccacatc ggcacccgag ttcatggtgt tgagatccaa tgaacaaatt
      241 tctggtttta gtgctgcgac atgctcgact cgtttgagnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttaccggtc tcggggtaac gcacatggat
      421 atgcactact gcggctccgg catcagcagc ccctaggcag gagtctgcaa tctgctttgg
      481 ggtaatgggc agatacgggg tcatctcagg tttggtgaga ttacctgtta ctgcgcaagt
      541 taggattgtt ttgttcattt gggagctcac tgttcttatc aattgatcat gtcatttgag
      601 caaactttgg tgagtcgagc aatacgttag tacgtatcga aagaaattgg cggagcggac
      661 gggactcgaa cccgcgaccc tcggcgtgac aggccgatat tctaaccaac tgaactaccg
      721 ctccgtatac agcatcatgc ttgtcatcgc atgactggcg tccccagggg gattcgaacc
      781 cccgtactca ccgtgaaagg gtgatgtcct aggcctctag acgatgggga ccttggacta
      841 ctaaaacttg tcttacttct actactttgt ttggtggtgg agataagcgg gatcgaaccg
      901 ctgacctctt gcatgccatg caagcgctct cccagctgag ctataccccc tcgcgtctac
      961 gcacttccaa aacaatccaa gattttagcc tatttttgct agacgctcga ttacttcctc
     1021 tttacccaat actgcaatca cagcatcaag ggctggggtc tgggtcgtag caaagagcgc
     1081 ataccgaatt ggcatggcta aagcaggcat tttgagattg tgttgagtca gcatggtctt
     1141 taagagagcc ccaacctgct ccttgctttg ggcttcttga gtctttaacg cttctctaaa
     1201 ctcggcgatc gcgggccgca cattttctgg gatgttctct tggatttgnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntctttcagt
     1381 aatgccacca cggcctcaaa gttagggcct tgggcgatgt tgatactttg ttgatcggca
     1441 aatggcttac ataattgagc aaggcgctta ggatccccgt tttggatgta atggtgattg
     1501 agccaaagga gtttctcggg attatgttgt gcgggtgatc gacccaaatg ctctagatca
     1561 aaccagccaa tgaactgatc tttactaaag atctccacat cgccatgtga ccaaccaagg
     1621 cgagctaggt aattaagaat ggcttcgggc aagtagccca tcttttggta atcgcgtacg
     1681 cttagtgccc cattgcgttt actcatcttc tcgccactct catcgagtac ggttgggaga
     1741 tgtgcataga ccggtggtgt accaccgagg gatttcatga tgttaatttg tcggggggtg
     1801 ttgttgacat gatcatcacc acggattaca tgggtgatct tcatatcgag atcatcgacc
     1861 accacacaga agttataggt tggagtgcca tctggtctag caatgattag gtcatctagc
     1921 tcttcattgc taatctcgat catgcccttc acagcatcgt tccagatcac cgatccccca
     1981 attgggttct taaagcggat aacggggttc acatccttgg gaggtgtggg taaggtctta
     2041 ccagactcgg gtctccaaaa tccgttgtaa cgcggtttct gtttattggc catctgctca
     2101 tcgcgcaggc ggttgagctc atcctcactc atataacagt aatacgcact gccatcggca
     2161 agcatctgct tgagaacctc gcgatagcga tccatatgct gcatctgata gatggggccc
     2221 tcatcggggt ccatttctaa ccagtgcata ccctctaaga tcacctcaac ggcttctaac
     2281 gatgagcgct cttgatcggt gtcttcaatc ctcagaatga aatctcctcc cttgctgcgg
     2341 gcaaatgccc aagggtaaag agcgctgcgg aggttaccta aatgaataaa gccggtgggg
     2401 ctaggggcaa atcgagttcg aatacgcatg gggattatta tctcaggtta tgaatgagct
     2461 tttggtcttt gtctgcgatg gcgctccggt gcgcggtgag tttgtgtctt tgggcagtac
     2521 ttggcaaagt attgtggagc gtcgcaatga tcctcccgcg gtgcgcgctc tcatgggtga
     2581 atttgtggcg gcagctaccc tgctcactgc cagtattaag cttgatggca cattaattat
     2641 tcaggcgcag agtaagggtc ccattcagtt gctagtggtg gaatgcactt cgcaactcga
     2701 ggtgcgagcg accgtgaaac ttaatcccaa tgtcaccgag atccccgata acgccacctt
     2761 ggcggagtta ctcgatgccg acgggagtgg gcgtctagcc attaccttag accctagtga
     2821 tcgcaaagcc tcacaagccc cctaccaagg catcgtggca ctaaatcggc catcgccttc
     2881 gcatcccggt ttactagaac caattgagtc ggtcagcgag gccatcatgc tctatatgca
     2941 aaactcagag caattggata ctcacgtatg gttagcgtgt ggccctgact cagtgggtgg
     3001 ctttttatta cagcgcctgc ccgatacggg gggtgcatcg caatacgacc ctgtgatgat
     3061 gagtgaaggt tgggagcgca tccagatctt gggtaatacg attaccaatg aggagttgtt
     3121 gacggtctcg ccagagaccc tcttgcgccg cctcttttta gaagagtcca cgcagtatgg
     3181 ggtgcgtagt ttcccgactc gtttagtgcg ctttgcctgc cgttgctcac gacaacgtgt
     3241 ggccgatgtc attcggatgc tgggtcaaga agaggttgat agcatcttag ttgagcaagg
     3301 aatggttgag accacctgtg atttttgtgg gatgcagtac cgctttgatc cggtggactg
     3361 taagcaagcc tttgctagta caacccttac tgatgcnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnncccgc tattgtcttt aggcacgatc tgtacaaagt actcgccctc tttgatcatg
     3481 ccatgctctg tacgcgcacg ctcttcaatg gctagctttc cttctttaag atctttaaca
     3541 tctccggcca acttgttatt acgtatctca agtgctgcat ttttatcttg ctgggcctca
     3601 agctggcgct cgagctcata caccttgatc cagccgccct taccaaacca cagtgggtac
     3661 tgaataagga tcagtaaacc cagcatggaa tagataatca ggcgcatgct gtgttttgct
     3721 tatttaccca aattgcggag gttataaaac gcagccttac caggatagct tgcaatgtct
     3781 ccgagatctt cttcaatgcg caagagttgg ttgtacttcg caatccgatc agagcgtgag
     3841 agtgaacccg ttttgatctg acctgcatta gtgcctaccg caatatctgc aatggtgctg
     3901 tcttcggtct ctcccgagcg atgcgagatc actgcggtgt aattnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc ttatcaatac
     4021 cttctttgag aatgcgcgtg ttggtcacaa atagatcatc accaaccaac tggatcttct
     4081 ggcctaattt ttgggtgagg gtcgcccaac catcccaatc tccctcgtgc ataccatctt
     4141 caatcgagac aatggggaac tgatctgcta ggttggctaa gtaatcagca aactcggtgg
     4201 aacttagttg caaaccttct ccagccaaat ggtacttacc atctttgtag aactcgcttg
     4261 cagcacaatc aagcgccagt agcacatcgt caccggcctt gtaccctgct ttctcaatgg
     4321 cactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 naggattttc ttgagcgcat gaaatatttc tgcgccacag cgcagtgctt cccgaaaact
     4501 ctcggcaccc acgggcatga tcatgaactc ttgaatatcg agactgttgt tggcatgcgc
     4561 cccaccattc acaatattca tcattggtac ggggagttgc atggcacctg atccgccaaa
     4621 atagcgatac aggggtaagc cagcttcttc tgctgcagca cgagcaacag ccatagatac
     4681 cgccaaggtg gcattggctc ccaacccggc tttgttgtgg gtgccatcta gatcattaag
     4741 ggtgcg
//
LOCUS       DDPQ01000002            5513 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_21836,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000002 DDPQ01000000
VERSION     DDPQ01000002.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_21836"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    989..1000
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2796..2876
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4961..5012
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5321..5352
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1403 a         1188 c         1271 g         1474 t
ORIGIN      
        1 gcagaaactg cttcgtcagt taacaagcca aaactggctg atggtaaaaa taagaagcct
       61 gtcttgggag ccctcttggg cggagcgaag aagtaaatta ctaactatca cttcgcttta
      121 gatctgtggc ggtctaaagt cgagagattt acattcttag ccaattctct agcttaagag
      181 ttttaaggtg tttaaaagct agatcatcac ttacaagggt catattgttc gagacagcat
      241 gactggcaat gagaagatcc aaagtatcaa tggatttacc ttgctgctcc aaactcgccc
      301 ttaactttcc atagacttca gcggtagagt gatcccagct cagaacatca atacgtttaa
      361 gcagttcatt tattgtgttt ttaagtgtct tagccccagg cctttttgct aagccatatt
      421 gaatctccgc atacgtaatc gacgagatat aaattgctga cattgggatg gataccagtc
      481 gtttaatcac tttttgttga ttttttacaa tatgactgat cgtattagta tcgagcaagt
      541 aacatttcat tctgccaatc ccttaaatgg atcccgagac gataattttt gcttgcgctc
      601 tttcttgcta agaaaatctt tgggcgtttt taatgtgtcg agcattgctg tgaaaccaga
      661 ccaatcagcc ggtttagcag aaataattaa attacctgta tctggatctt tatccaaaaa
      721 tacttcggtt gagttaaagc gaaactcttt aggtagtcgg attgcttgac ttcgtccatt
      781 ggtaaacaat tttgcagttt gcttcatatt gggactcccc tatatgtttg gtatatatca
      841 tagtatatac caaaatacag ttcatcagag cggccctttt ttgtttaatt gggcaataca
      901 ttcatcaatc aaaacaatga taggttttga atcaccatcc tttacgttgc caaattttaa
      961 ttgctatgga taaccattca tcaatagtnn nnnnnnnnnn cccaaatatc tagatgactg
     1021 gcagctgtat tcaatgcgtc gagagttgcc tctttagtgt ttgtattaat tcctaaagcc
     1081 tgagtttgct tactcaactt ttctcctgca gcattcgtga caagtggcag gtgtagatag
     1141 ctaggtattt ggtaccccaa tatctgttgc aaatagagtt gccgcgctgt attacttaaa
     1201 agatcttctc cccgcacgat atgggtaatg ccttgaagat gatcgtcaac cacaacagcg
     1261 agttggtagg taaataatcc atcggcacgt cgaagtacaa aatctcccac ttgcgtcgta
     1321 aggtcttgcg attggacccc cttcatgcga tccttaaatt gcaatacgca tgagcctggt
     1381 aaggctactc gaatagcagc tttttcttct tgcctatttt gattacgaca gctacctggg
     1441 taaatgagat cttcgtgacg ccctagttca gccccacttg ctcttaaagt ctcctcaatg
     1501 tgtttgcgtg agcaaataca gcgataagtc atttgatctt tgtttagttg atctaatgcg
     1561 gcttgatata gggctgtccg ttttgattgc cagaccactt cttcgtccca ataaagaccg
     1621 caggcgatta gctgttgcag aatgatctga tcggagccat ggacagttct tggggtatcc
     1681 acatcctcaa tgcgaaccaa ccattgaccc ccatgagctc tggcatcgag ccagcttcct
     1741 aaagcacttg ctaaggaccc agcatgtaaa gggcctgtag gggatggcgc aaaccgaccc
     1801 cgatagggga aggcttggcg agacgtctct tggctagagc cttgactgga ttgaggattt
     1861 tgcagggtat tcatttagac caaattttgg ggtgctcaca gctaaaatga tctcatggct
     1921 gcaccccaat ttgttcatct tcgccttcat tccgaatatt ccattaccga tggggtggta
     1981 cgcatcgacg atgccattgc tacagcctct gaagatggca tgggcgcatt agcgctcacg
     2041 gatttgggta atctctttgg tttaatcaag ttctattcag cagcccgctc tggaggtatt
     2101 aagccaatca caggagccga tatttggcta agtaatgagc aggagcccga tcagccgcat
     2161 cgcatgttat tgcttgtcca aaatcactct ggctatctta atttgtgcga gttgctaacg
     2221 cgagcatcac tccacaatca acatcatggt cgagcagaga tccatcccaa atggctctta
     2281 gaacaagcta ccaagggtgg cgctcaaaag attggggagc aaaaatccgg acagacattg
     2341 gcgtcgggtt tgatcgccct ttcaggcgct catagtggtg atgttgggtc cgcattgtta
     2401 gctggtaatc cagaacaggc tagagcgctt gccacccatt ggcaaacggt atttgatcat
     2461 cggtacttta ttgaggtaca gcgctcgggc cattcccaag acgaggctca tattcagttg
     2521 ggctgtcagt tagccagcga actacagatt cctgtcgtgg ccactcaccc cattcagttc
     2581 atgaagcctg atgatttcat cgcgcacgag gcacgagtct gtattgcaga gggagagtta
     2641 ttgggtaacc cacgccgccc aaaattgttt actgagaatc agtatttttt gacccaagca
     2701 caaatgcaag agcggttttc tgatctgccc tcagcgcttg ctaattcggt tgagatagcc
     2761 aagatgtgta acttaacgct gaccttaggc caagcnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnataa
     2881 aaatcttctc aaactctatc ccgacaccga agagcgaaca tccattgagc cccgttattt
     2941 tgatcgcctc aagtttgaag tgaacaccat tgcacagatg ggtttcccag gctacttctt
     3001 aattgttgcc gactttatta attgggccaa gaataatggg gtgcctgtcg gtcctggcag
     3061 gggctctgga gccggttcct tagtggcatt ctctttgggg attaccgacc tagacccctt
     3121 gcgttacaac ctactctttg agcgctttct aaatccagag cgagtctcga tgccagactt
     3181 tgatatcgat ttttgtcagc atggacgcga tcgggtcatt gcctacgtaa aagaaaaata
     3241 cggcaaggat gcggtcagtc agattgctac ttttggaaca atggctgcca aagcggcgat
     3301 ccgtgatgtg gggcgcgttc ttgagcagcc ttatggtttt gtggatggta ttgcgaaact
     3361 cgtccccttt aaacctggtc aggtggtcac gattgaatcg gctaagaaag aagaaaagca
     3421 gctagcagaa cgcgaaaaga atgaagagga agtaaagcaa ctccttgcac tcgcgcaaca
     3481 acttgagggc atgacccgta atgtggggat gcatgccggc ggggtcttaa ttgcccctgg
     3541 aaaactcacc gatttttgtc ctctttatac gcaggatgca aaagaccaag acgcaggagt
     3601 aattagtcaa tttgataagg atgatgttga gtccattggc cttgtaaagt ttgacttctt
     3661 gggtctgacg actttaacca tcctggcagg agctgagcgc tggatcaaag cgctgcatgc
     3721 agagcgaaag aactggagta ttggggatat accgctcgat gatccagcgg catttgagct
     3781 cttaaagcgt ggtaatacgg tcgctgtgtt ccagttagaa agtcggggca tgcaaggcat
     3841 gctgcgtgat gccaaaccag atcgctttga agacattatt gcgctagttg ctttatatcg
     3901 tcctggtcct atggatctta tacctgatta cattaaacgt aagcacggtc aacaacgggt
     3961 tgagtatccg gacccacgga ttgagcctgt tttgcgagag acctacggca tcatggtgta
     4021 tcaagagcag gtcatgcaaa tggcacagat gataggtggc tactctttgg ggggtgcgga
     4081 tctcttgcgc cgtgcgatgg gtaagaaaaa gccagaggaa atggcgcagc atcgtaagat
     4141 ctttcgagag ggcgcccaaa acaatgggtt aactgaatat aaagcggatg agatttatga
     4201 cctcatggag cgctttgctg gctatggctt taataagtca catgcagctg cgtatgcact
     4261 actcgcctat caaaccgctt ggctaaaggc ctactatccc gctgaattca tggcggccaa
     4321 cttatcttta tcgatggatg ataccgataa gatcaagatt ttgtatgacg acagtttgca
     4381 taatggccta aagatactcg cccctgatat caatactggg atgtatgagt tcacaccgat
     4441 tatggatgag gaaaacactg aacaagcacc cataagcact caacagacgc aacatcggtc
     4501 tcctttgaga acgattcggt acggtttggg agcggttcga ggaaccgggg agagcgctat
     4561 ccagattatt attgaagccc gcaagaccgg accgttcaaa gatttatttg atttctgtgg
     4621 cagagtcgac cgccgtcaag taaatcgccg agcaattgaa gcactgattc gtgcgggagc
     4681 atttgattct attgctggag atgctgcaaa gggctatacg cactcctatg aggcgcgatc
     4741 aacactttta gcatcgctct cacgcgcgat tgagtctgct gagcaggccg aggcatcgat
     4801 tcatcaggtt agcctatttg atcttggtga cgattcagag agacatgctc cagagtttgt
     4861 tcgtgaatta gcatcctccg agaaaaagcg cctacaagat gagaagactg ccttaggctt
     4921 atgtctgacc ggtcacttat tcgatgccta tcgatccgaa nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggaattat cacctcctcg cggatgctga
     5041 tgggtcagcg tggccgcatg atgatcgcaa cgattgatga tggcactaca gcacttgaat
     5101 taacactcta cagtgaagta tatgaaccca accgatcttg gttaaaagaa gatgagttat
     5161 tgattgccaa ggtaaatgtt agcccagata agttctcagg aggtttacga gtggttgctg
     5221 agagtgtgat ggatattgtt ggtgcacgtc tacgctttgc cagaaatgta cacctcaatc
     5281 tagattcggg catcgatcta aaggtgctac gcgcccaaat nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnacagccgc agtaatggca aaaggcggcg catgccttgt tcagttccca
     5401 gatgagatgc gtctttatcc tgatgataat tgcttgcgca atctgcatca aatcttaggt
     5461 aataaatccg gattcaactc accaatcact ttggttgaaa ttcaatacgc cta
//
LOCUS       DDPQ01000003            8186 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_8294,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000003 DDPQ01000000
VERSION     DDPQ01000003.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_8294"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1632..1694
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2083 a         1841 c         1942 g         2257 t
ORIGIN      
        1 gccagaattt tcccagtaag gtttgtgatt ccaggaacaa tatcttgata gcgcttaacg
       61 gagacattat taagttttcc ggagacgtca aatttaggcc cttgcccact gattaggcca
      121 ccaacaagcg gaatagttga tttgttttca gaccaggtaa tggatacatc gtttagttcg
      181 ccccttggct cgctttgctc aatgatttgg cgaatcctgc ttggcagggg caaatttaag
      241 gcaaagagac taaggttttg taagccaatt ttggaggaag aaaaagagaa cttttcgatc
      301 tccgtatttt tcttcggaac ttgccaagaa aatgtcatgg gcgccagact ttcaggggct
      361 gtgttggagc cttggttctt ctcgcgccat gtaaatcgtt gaatgcctaa tgaaacaaat
      421 ttaccagagg taaattgttt ggcatctaac tctagctggg caaattcaat cgcttgatta
      481 ctttttgatt gttgaaatat gggacgatca atcgacagag cgaactgacc gccatctggt
      541 agtcctttag aaagagagag agctccggaa gagcgtaaga ccccgcttag ttgtttaaag
      601 gggatttcaa aatcacgggt taattggcca atatcaagtg attgcacatc ccattgaaaa
      661 tcaccaatcc aatcgcgcca atttccaggc tcaccaccga tacggtgtac aaattttcct
      721 gaaaggctca gtttctcttg gtgccatgga ctaaagaggc cgatattgag ttcgtgagta
      781 cgaattccac tgcgaagggc taaggtctca atacgcagat ctgctacatc ggatttcgat
      841 ttagaaaagt ctttccacaa aatagtcgca ttattgagtt caagattatt ttgtgaaaac
      901 agccagtttt gggtatcgag atcatcggcc ccagtgccag ccaagatgcc cgcaacaaat
      961 aagcgctctt gtttatcgcg aaccacttga atgattgcat cgctagcctg cagtttgttg
     1021 aaatggggtt taaggtgata aacagacgac cagcttaatt ccccccgaat ctcccgaatc
     1081 ttgagcccag ggctattggg gatgagtgct gtttgttggt ttgggttagg aatgaactca
     1141 aggccggtca tgtcaaatac gggccgaatt ccttcccaat gtacatgcag gtggtcaatc
     1201 ttgaccgaca ccccaattcg ctccgagagt aatttttcaa tagcaggctt tgaggtttct
     1261 atctgaggcc agacgacaaa acgaatcaat agatgcccaa tgaccccaag gctcacaata
     1321 attaggccaa gtatcaatag gcgtcgagac cattgagccc aagaactggt ggtgggccgt
     1381 aacctaagga ggtcttttag gcgaagtggg tactttttct cggtcatcaa tccctattat
     1441 ccgacacccg aattcaattg ggctagtaaa gattaagatt agatatggtc aatacccaaa
     1501 ctgctcttac ttttctggag aaccactcct tatttgctag ccgttggcta ggggcacagc
     1561 agcaatggcg aggctggctt gaggagagac ttcatacccc cattgagcct aagcagatag
     1621 acggattgct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnncttgcc cgccagcgct taatgctatg gattgcgcat cgagacctta
     1741 atggcttagc tactcttgat gaggttacgc atgccctgag ttactttgcc cagcagagtt
     1801 tggcattgac ggtagcttat attcgccgag atcttaatag ccgatttggc ttgccatggg
     1861 ctcgtttagc tgactacgag ttgccgctca tgattgtggg catgggtaag ttggggggaa
     1921 aggaactcaa tctttcatcc gatattgacc taatcttttt gtatgaagaa gagggcgata
     1981 cccgaggtgg ctctagctcg atctcgaacc acgagtggtt tacaaaactc gggcgtcgcc
     2041 ttatcagaat tatttctgaa cacgatgtca atggttttgt ttttcgggtc gatatgcgac
     2101 taaggcccaa tggagattct ggcccattgg tttgtagttt agaaatgctc gaagaatatc
     2161 tctttgttca aggtcgagag tgggagcgct acgcttggat caaaggtcgc atgatttatc
     2221 ccctaaaaga tcaccccgat cacgctcggt gtgaaaaggg tctagagcaa attattcgtc
     2281 catttgtcta tcgtcgtcat ttggactatg gggtgattgc agcgattcgt gagttacatg
     2341 cacaaattca gcgtgaagca gagaagcgaa gtagcaaccg tagtggccga tcgcgggata
     2401 ttaaattagg ccgaggcgga attcgtgaaa ttgaatttct ggcacaaatg tttcaactca
     2461 tgcgtggcgg cacagatcct cgtttacgga ttcgcccaac cttagaggtg ttagatctat
     2521 tagaagccaa tcaactgatg agtcctgaag aaattcatga cttaaagggg gcttacgtat
     2581 tcttacgacg cctagagcat cgaatacaga tttgggaaga tcagcaaacc cattattttc
     2641 cagaaagtga tgaagcgcgt ttacagcttg cccaggctat ggccggacca gatcaagtca
     2701 ttacgctcga ggcattttta caagagttca acgatcacca gaatcgagtg gcacgttact
     2761 ttgagaaagc attctctttg gatgcgggcg gtcgtttgca actcgatgca aacgatatgg
     2821 attggcagcc cgatcccgct ctatttccaa aagtgcacga gcgttggcaa agttggcagg
     2881 atagctctcg cgtaaaggct ttaccagaaa aaagtcgttt ggtgatccga ggtttgctta
     2941 aaaaagctgc aacagatatc actgtctcaa atgtcggtga cgcagatcaa accctattac
     3001 gtttctttga cttgctcgaa gcaatttcta ggcgcggtgc ttatttatct attttggcag
     3061 agtattccaa tgcattacaa aaggtgcttt tactcttgag ctcctctcaa tgggcggctc
     3121 agtatctcgc tagacaccct cacttattgg atgacctttt atccgctagc gctcagtcag
     3181 agctaatcca cgatccagat agttattgga aaaaggtcaa gatcgatttg gatatgcgct
     3241 tagatgacgc tcttgatgat catgcaagcc ctgatcatgc aatggatatt ttacgtatta
     3301 cacaccacac cgagaccttc ttaattctct tagcagattt ggggattggc gccccagagg
     3361 gtttaagtgt cgagcgagta agtgatcgac tctccgctct agccgatcta attttgcagg
     3421 ttacctatga acgtgtttgg ccaactgttg cccaaaagtt ttctgtggat gccaagaaat
     3481 taccgcagtt tgcagtcatc gcctacggca aattgggtgg caaggagtta gggtatgcat
     3541 ccgatctgga tctcgtattt ttatacgatg ccccagcgag tgaccaatca gctcaagagg
     3601 ttttttcaat cttgggcaaa cgaatgatta attggctaac aaccttaaca gcagcaggca
     3661 ctttatttga gattgataca cgcttacgtc cgaatggcgc agcaggattt ttggtgacca
     3721 gtctagattc gtttagacga tatcagttgc gcgaaggtga taatgcggca tgggtttggg
     3781 agcatcaggc gatctcacgg gcacgctttg cagcaggcaa ccccgatgtt ggcaagcgct
     3841 ttgaggagat ccgtaaggag gttctatcgc aggaacgtaa aacgaatgaa ctcaaaaatg
     3901 agattattga gatgcgtcac aaggtccatg cgggccatcc aaatgcatcc tcagactttg
     3961 atctaaagca tgaccctggg ggaatggttg atattgagtt tatggtgcaa ttttttgttt
     4021 tggcctatgc gaagcaattt cctgctttac taggtaattt agggaatatt gcattactcc
     4081 gaattgctgc agagcatgaa ctcattactg atgtacaggc cttagaaatt gccaatgcct
     4141 atcgaatatt tagggcgcag caacaccgct tacgtttgga tggcgcagaa aaaacgcgca
     4201 ttgatattga ccaacaccct gaatttgcaa acgccagaac ctacgttagc gctctctgga
     4261 atcaagtctt tggtgcgcca tcacgcccct aagtgaacaa cttaagactg accaagaagt
     4321 tctcgagcgt gacgacgagt ggtatccgta atggtggtac cgcccaacat tctggcgatt
     4381 tcttcaattc gttcttgccg tcctaaagaa cttacgtcag aactagtttt tccactcatt
     4441 tgtgtttttg cgactttaaa gtgatgattg ccctgagcgg caacttgtgc cagatgggtt
     4501 acgcacaata tttggtgaga ttgacctagc tgcttgagta gctcaccaac ggtctgtgcc
     4561 actgcaccac caattccaga atcgacctca tcaaaaataa gagtaggggt aaagcttgcc
     4621 tggctggtaa tgacactgat cgctaggcta atgcgcgcca gttcaccgcc agaggctact
     4681 tttgctaggg ggcggggagt gcttccagca tgcgctgcaa ttaagtactc cacatggtca
     4741 atgcctttgg cactcggctc agatgggctt aacataatct gtagttgacc tccagacata
     4801 gccagcgttt gcatggcagt gctaatctct ttgctaagct gttgagccgc cttctgacgt
     4861 ttttgcgaga gctctttcgc aagctttatg aacttggctt cttgatcttg aaattgctga
     4921 cgtagtactt ctatattttg cgcagcagtc aatgcgtcta atttttcttg cgtatcgatc
     4981 caaagttggg ctaaatcgtc tggattcact ttatatttac gtgctgccgt atgaagtact
     5041 tgcattcgcg catccacttc cgcaagtcga tctggatcca aatccatttt ttgcaaataa
     5101 cgattgagac tatggagagc ttcatccaat tgaatctggg caacgtttaa actttctgct
     5161 acttgatgca gggcagggtc atgctctgcg agagcatcta tttcttgttt ggcttgattc
     5221 agttgcgact caatggagtg gtctgattca ctgagaagat caattgttgc ctggcttccc
     5281 tgaataatct tcgctgcatt agctaagcgt gagtgatcat ttttgatttc ggcccactca
     5341 ttagcttgcg gggcaatatc attgagttcc tcgagttgcc attccaaacg ctctcgttct
     5401 cgttgtatat ctgcgcccgc agattctgct tgctctagct ttttctgagt ggcactcata
     5461 gccttgaatg attcagaaac ttgggttatt agatcatcca gtccagcatg gcgatctagc
     5521 aactcccgtt gcgcacctgt ttttagaaga agctggtgcg catgttgccc atggatatcg
     5581 accagacggt cgccagcctc acggagttga tttaaagagg cgctactgcc attaataaaa
     5641 gaccgactac ggcctgtgtt gtcaatactt cgttttagga tcaggctttt acccccatcc
     5701 tcaagaggta ggtcatgcgc gcctaaccac gtattcattt ctgtttctag tcttttgtct
     5761 atcctaaaaa tagctgtaat ttctgcgcgc tgacagccct cacggatctg gctgctgtct
     5821 gcccgctcac cgagtgccaa tccaagcgca tctagcagaa tcgatttacc cgcacccgtt
     5881 tcacccgtta aaacactaaa gccctctaca aagtcgagct caagttggtc gacgattaca
     5941 aaatcacgta aagctaatga ttgaagcatg tctatctacc cagattaaaa cgtagaagga
     6001 tattcgttcc aatgcaattt ttctcgtaag gtttggtagt cactatgacc caaaggatgt
     6061 aggagagaaa tagatttttc ggagagccgc acttcaacac ggtcgccaat ttgtaattta
     6121 gtaagcgatt gcatgtcaaa gttgacaatc acttcacgac cgccaaccac ctcgatcgca
     6181 ataatggatt cttgagcgag gacaatgggg cgattggata gggcatgagg tgcaatggga
     6241 accaaaacga tacccggaac cctaggatgc aaaattgggc cgccagcaga cagggcgtag
     6301 gcggttgagc cagtcggtgt tgaaacaatt aagccatcgg agcgttggtt atacataaac
     6361 gactgattga catggacttt aagttcaacc attccagaca ggccggaacg atttaccaca
     6421 acatcattta gggctaatcc acgatggatt tctttgccat ttcgagagac ggcagcctct
     6481 aagagcgagc gagagtcgat ctcatagtgc ccttggagga tttcagggag gaccgttttt
     6541 gcatcctcaa aagggatatc ggtcatatag ccaaggcgcc ccatattgat gccaagcaga
     6601 ggaacatcgc ttcctgcaat ttggcgccca atacccagca tggtcccatc acctcccaaa
     6661 atgaccgcca aatcggtgga atctctaaag tcagacagac gcagggtttt gctgcctttg
     6721 agctgtagat gagccgccgt ttcagactca atactcagct cacagccttg ttggataagg
     6781 atattagcta attcctgtag atgctggcca ataccatcgg cctgatgctt accaactaag
     6841 gtaacgcgtc taaatttctt ctgtaaaggc tggcttgaag ggcttaacat acaacgatta
     6901 aaccataggc ataaaattac tccaatcatg gatgatcgct ccaaacttct gctgaaaacc
     6961 ctcatcgaga gatacatcga ggaaggacag ccggtgggct ccagaaccct atcccggttc
     7021 tctggccttg atttatctgc cgctaccatc cgcaatgtca tggccgacct tgaggagatg
     7081 ggttttgtaa cgagcccgca tacctctgct ggccgtatcc ccaccccccg cgggtatcgc
     7141 ttgtttgtcg atacgatgtt gaccattagg cctttggagg aactagcatc gagagaggtc
     7201 gagcgttctt tgtatccaga ctcccctcag aaggtcatta attcagcagc gcaactttta
     7261 tcgagcctga cccattttgc aggcgttgtc atgactccca agcgctccca gatttttaaa
     7321 cacattgagt tcttgcgttt gggcgaggga aaaattttac tgatcttggt tacccctcaa
     7381 ggggatgtgc aaaatcgtat tttgccgacc acccaggatt acaccccaag tcagttggtc
     7441 gaggcaagta actatatcaa tgcccatttt tctggaaaga gtttttcaga tgtcagcgct
     7501 cgattgcgtg ccgagcttga tcacttgcga ggagatattg cgggtttaat gaccttggcg
     7561 cttgagaatg gggtggcaga ttctagatta acgcatcctg atatggtgat ctctggggag
     7621 aggcatttat tggatgtggg tgagcttagc tctaacttaa gtaaattgcg caagatgttt
     7681 gacatgcttg agcagaaatc agttctgatg caattacttg acatctctag ccacgctgat
     7741 ggaattcaga tatttattgg cggtgaaagt gagttattac cctatgagga tttagcagtg
     7801 atcagcgcac cctatagcgt cgatggcaaa atagtgggca ccttaggggt tattggcccg
     7861 acaaggatgg cgtatgatcg cgtgattccg attgtggaca ttacttcaaa gcttttaagt
     7921 ggcgccctga gtactactta attatttagt ttggatttca tttgaacacc agccctcatc
     7981 ttcgttctgc tcgtactggt attttggtaa tcaatctggg cacaccagaa gcaccgactc
     8041 gttcggcagt gcgaacctat ctcaagcaat ttttatcaga cccccgtgtt gttgaaatcc
     8101 cacgcttggt ctggtggttg attttgcacg gaatcatttt gccaattcgc agtggcgctt
     8161 cggccaaaaa atatgcctcg atttgg
//
LOCUS       DDPQ01000004            3550 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_34761,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000004 DDPQ01000000
VERSION     DDPQ01000004.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_34761"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1237..1318
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1570..1625
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1003 a          895 c          678 g          836 t
ORIGIN      
        1 tcaataaagc cacctaaacc aaaaatagta ttgacgacca cgcgcattaa atcactaaag
       61 gccatactaa agttgccctg cagaagatta ttagctgagg tatagatatc ccgatagtta
      121 ccaaagaaat tatcgatgcc ctgttgtgct ggttttggca gaataaaacg atagcctttc
      181 gtaatgggct ttagtaaata atcatccaat ccttcgttaa aagcgaagac cgagcgatta
      241 aaagattccc aaggatcatt gggagagcgc tcactgccag gcggaatgct tgcgcaccca
      301 atcatcgagg cagaaataaa gagaagaaaa agacgtttta aacttgacac agagttgtta
      361 ttgttatttt ggtagagagt ctaattctac ttcgatgaac ccttggcacc atcgccttta
      421 tcttgcccgc tatcggcagc cttgttatac aaaaactggc taattaaatt ctccaagaca
      481 atagctgatt gagtttgggc gatcttgcca ccttcaccaa gcatttgctc atctccacca
      541 gcctctaggc caatgtattg ctctcctagc aaacccgagg ttaatatttt ggccgaggag
      601 tccttcggaa acttatatgc cttatcaatg gtcatggtca cagtcgcttg atacgttgag
      661 tcatcaaact tgatatccgc aatgcgccca acaaccactc cggcactttt aacaggggcc
      721 ctaggcttta gaccaccaat attgtcaaag cgggcagata cggtataggt gggcgcaaat
      781 gagatggcgc tcatattgcc aaccttcaag gccaagaaca acatcgccaa catgccaatg
      841 gcaacaaaaa tacctaccca gacatcaatt gcactttttc tcataagaac ctcagtttat
      901 tctgaatgga acaattactg cctaaaccat tcatttttat attcactctc aattgtttga
      961 gaacatcatg gcggttaata aaaaatcgag agccaatact gctaaagatg acaacaccac
     1021 cgttttggtc gtagcctgtg atacgccctc aggggttggc ttagcctcat agccctgata
     1081 tagagctata aaggtcaccg caaagccaaa cacaatgctc ttaataaaac cattgccaat
     1141 atcatcaaac aaatcaacac cgccttgcat ctgcgaccaa aaggcgccgc tatcaacacc
     1201 aattagtggc acacccacta aatagccgcc aagaacnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     1321 agcaactaat aagcctagtg cctgttctga gccataacgt gtaagtgtgt aataaccctg
     1381 gaggcctaaa acaaagccta caaataaacc ggagaccgta ataatgacaa aagagtagtt
     1441 acccacaaat aagacttgat ctgaaactaa acgggggcgc tttaataaag atcccgatcg
     1501 gaatagcaca agagcaaaca tacgagccgc ataaccaaga ccagtaatat tgcgacgaat
     1561 aaaaaatccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnttaaa gggaaccggg ccctccggtg aggcatctaa aaactgacgc acaaaaggat
     1681 cgcttgagcg attgagatca tctggggttc cctctgcacc gatttttcca ttcgcaataa
     1741 agtaaacgta gtcggcgatt tcaaaggttt cttccacatc gtgcgtaacg ataatgctag
     1801 ttgccccaag cgcatcattc aaattacgga ttaagcgggc tgtaatcccc aaaccaatag
     1861 gatctaatcc tgcaaagggc tcgtcataca tgatgagtgg tgggtctaga acaatagcgc
     1921 gggctaatgc aacccgtctt gccatgccgc ctgatatttg tgaaggcatc agatcacgtg
     1981 caccccgcaa cccaactgcg ttgagcttca tcaagactag atcgcggatg agctcttcgc
     2041 tgagattggt atgctcgcgc aatggaaaag cgacgttctc aaacacactg agatcagtga
     2101 acaaagcacc gaattgaaac agcataccca tgcgtcgacg ggcttgcatc aactgattgc
     2161 cagtcatcgt accgacatca tggccttcaa agtgtactga gcccagttgt gcaatcactt
     2221 gaccgccaat cagacgcaag actgtggtct tgccacaacc cgagccaccc atcaccgcaa
     2281 cgacttgacc gcgcttaaac tccatattga gtccggagag aatttttctc tctccagggg
     2341 cgtaggagaa atctaccccg cggatagaaa ctaccgcagc atgatcatga tttgcaatag
     2401 acatgcctta ttatcgtggc aaaaccgacg agcccattaa aaactcatcg actgagcgtg
     2461 cacactgacg cccttcacgg attgcccata ccaccaaaga ctggccacgg cgcatatctc
     2521 ctgcagcaaa aaccttagga acatttgtgt gataagcctt atgaccatcg accgttgctt
     2581 tggcattacc ccgcgggtct ttctcaaccc caaaagcact aagcatttgt tgagcaggtg
     2641 agacaaagcc cattgccagc aaaactaaat cagccttgac ctcaaactct gaattgggga
     2701 cctcagccat cttcccatct ttccactcta gacgaacacc gataagtttc tcaaccttgc
     2761 catttttacc ttcaaaccgt tttgtggcta ctgaccaatc ccgctcacat ccctcttcat
     2821 gggatgaaga tgtccgtaac tttgtaggcc aatatggcca cactaagggc ttattttctt
     2881 cttctggagg ttgtggcaat agctcaaact gagtaacgtg tttggcacca tggcgatttg
     2941 acgtgccaac gcaatccgaa ccggtatcac cgccaccaat aaccaccaca tgtttatccg
     3001 tcgcacgaat ctcgttcttt aaatcgcctg caacctcttt attttgagga attaaaaatt
     3061 ctagagcgta atgaatgcca gctagttctc ggcctggtac cggtagatca cgaggctgct
     3121 ctgcgccacc actaataatg actgcatcaa agtttttcat tagctcttcg gggcttaccg
     3181 ttttctgagc ataatttttt acttccttac ccaaactatc cttgccaaca aaaacacctg
     3241 tggcaaaggt cacgccttcg gcttgcattt gctcaacacg ccgatcaatc aaccattttt
     3301 ccatcttgaa gtcaggaata ccgtagcgta ataaaccgcc cacgcgatca tttttctcat
     3361 atagagttac atcatgaccg gcgcgcgcta attgctgagc cgcagccata ccagctgggc
     3421 cagaacctac caccgcaact ttcttgccgg tcttgacctt aggaggctgt ggctctaccc
     3481 aaccattctc ccaagcctta tcgataatcg catgctcaat cgatttgata cctacaggta
     3541 gttcattaat
//
LOCUS       DDPQ01000005           11026 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_6401,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000005 DDPQ01000000
VERSION     DDPQ01000005.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11026)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11026
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_6401"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2802..2924
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5373..5403
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5957..6046
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7367..7425
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8621..8718
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2850 a         2349 c         2605 g         2821 t
ORIGIN      
        1 ctagtaagtc cccggataca agatatcttt cgtcacatcc tgaatatcac gatggccggt
       61 aaatgccatg gtgagatcta gctccctatg aataatctct aagcatttag taacaccagg
      121 gccacccata gcgcccaatc catataaaaa ggagcgacca atcatcgttc ccttggcacc
      181 caaagcccac gctttgagga catcttgacc tgaccgaata ccgccatcca tccacacctc
      241 aatatccttg cccgccgtat cgacgatgcc gggtagcgct ttgatgctcg atacagcgcc
      301 gtcaagttga cgaccaccat gattagacac aatgagggcg tcggcacccg attgggcagc
      361 ggcacgggca tcatcaggat ctaagatgcc cttgataatg agcttgccac cccaaagttt
      421 cttaatccac tcaacgtctc cccaattgag tcccggatca aattgctcag cggtccatga
      481 agatagtgaa gacatattgc caacaccagt agcatgccca acaatattac ggaaggtacg
      541 acggggtgtt tgtagcatcc ccatgcacca acgtggtttg gtcatcatgt tcaaaatgtt
      601 aggaatagtt aacttgggtg gagcacttaa gccattctta atatctttat ggcgctgacc
      661 taagatctga agatctaatg taagaactag ggctgagcaa ttggctgctt tagctcgctc
      721 gatcaagcgc tcaataaagc cacggtcttt catgacgtag agctggaacc aaaatggttt
      781 tgtcgtgcgt tcggcaacat cttcaatcga gcaaatactc atggtcgata agcagaatgg
      841 caccccaaat ttttctgcgg cttgggcagc aaggatctca ccatcggcgt actgcatgcc
      901 cgttaagcca gtgggtgcca atgccacagg cattgcaacg tcttggccaa tcattttggt
      961 tttggtggtc cgattgagca tattgaccgc cacccgttgc cgtaatttga tcttctgaaa
     1021 atcggattca ttggcgcgat aggtcgactc ggtccacgaa ccggagtcgg catagtcata
     1081 aaacattttg ggggtacgtt tttgatgtaa aacgcgcagg tcttcaatat tggtaatgat
     1141 aggcatgggg tatggagttc ggggttattt agtgaggtaa gaggtatttt ttgtctatta
     1201 tggattattg cttaattctg acggattgct ggatatgaaa cctagagtat tggcttttga
     1261 tgtatttggg acggttgtcg attggcatgg ctcgattact gcggaggttg ctagccttat
     1321 cccggagatc gatgccattt catttaccag tagctggcgt agtggttata aacctgccat
     1381 ggcgagagtg cgctctgggg agttgggatg gacccgaatt gatgatttac accgcatcat
     1441 cctcgaccaa gttttaaaag agttcagtat cgacaggctc accgaatctc agaaaaggca
     1501 tttgaatctc gtatggcacc gcttgcatcc atggcccgat accgtttctg gactgacact
     1561 cttaaaacaa gatcacacga tcgtgaccct ctccaatggt aatttagggc tcttggcaaa
     1621 tatggccaag aaagcgggtt taccttggga cctgatcttg tcagcagagg tatttcgtca
     1681 ctacaagccc gatccagaaa cctatttagg ggtttgcaat atctttgatc tagagcccaa
     1741 cgaggtgatg ttggttgcct ctcacaagga cgatctccaa gctgcccatg cctgtggtct
     1801 gcaaacagca tttattgagc ggccgctgga gtttggtccc aagcaagcta gagacgatct
     1861 gcatcgtgag acctggactg attaccatgc taccgatttt ctggatctcg ccaaacagct
     1921 aaaaacccga taaagaccta caatgaatat agaactactt tctcaaggag agaatgatgg
     1981 caaaccaaat gactgtggaa attgagaacc tgaagtgcgg tggttgtgag aacacgattg
     2041 tgaagggttt gatgtcgatt acgggagtcg ctggtgtaat tgtcgaccat gctcagaaag
     2101 cagtagttct ggaagctgat gcaaacttac gcccgcaggt tgtggcgaag ttgttgggca
     2161 tgggctatcc agaaaaagga tcagtggctg gactcgataa aggcattgcg actgcaaaat
     2221 cgtttgtgag ttgtgccatt ggtcgaatta gttaaagtag gctagaaggt ttttagatac
     2281 gctaagatcc catggcgctt catgttgaca tcattggcta cttagctgcc tgcctaacga
     2341 cattctcctt tttagttcag gcgataaagt cctggaaaac gaaggaccta tcgggcattt
     2401 ccttggggat gtacaccatg tttgctagcg gcgtagcttt gtggttggcc tacggcattg
     2461 cgatagatag catgccactg attgtgacca atgccttaac cctagcgttt gcccttagta
     2521 ttctgttcat gaagattcgg gaagtacgga aataaaatac atgcttacta gagtctaacc
     2581 atgaaaatcc tcaagatgat cattatttca gttgttgttc tagcggccat cgtcctcggc
     2641 gtagcctatt acagttcagg caagattgat gcgtatgtaa agactgcgat cgagcagtat
     2701 ggatcagagt cactgggtac taaggtcagt gttggggagg tcaatattag tttgcgtcag
     2761 actactgcca cgatcacaaa tcttgagatt gctaatcccc cnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcggg agatccaaaa
     2941 ggtaagaagg ataaggatcg ccaacaaccc agaattttga ttaagaagtt cgatattgag
     3001 ggagctaatt tatcgtaccg tgatactcgc attgtcagta caacggttaa tttaaagtta
     3061 gacgatattc atattactga tatcgataca ggcaagaatg gtgcaggtac acaagaagct
     3121 gtgagtaaga ttgtgaatgc gattgttcct gctgttaagc aggctgctat aaaaagtgtt
     3181 gctgcctatc ttgatgtcgc taccgatacc ttacgaaatg taagcgacag tgcccagcag
     3241 ttgggtaaag aggcagttac caatattaag aagttcttcc aaaaacagta attttaaatt
     3301 gagattaaat agtgaattcc actaccccta tctataacgg ccgactaaca tccttatccc
     3361 atggtggcgg ttgcggttgc aagatcgccc ccggtatctt gagcgatatc ttgaaggatt
     3421 ccccctttaa aacgatctca caagctttat tggcaggctc tgaaaataat gaggatgcgg
     3481 ccgtttatca aatcaatgat cggcaagcga ttgtggcaac cactgatttt ttcatgccaa
     3541 ttgtggatga tcctttcgac tttggacgga ttgcagcaac taatgcgatc tctgatgtgt
     3601 atgccatggg tgggcagcca ttgtttgcac ttgctctctt gggaatgccg attcaagtcc
     3661 taccgataga gactattcaa aagataaccg cagggggtga gtctgtctgt caattagctg
     3721 gaattccgat tgcgggcggc cactcgattg atagtgtgga gccaatctat ggcctagtgg
     3781 tcattgggat tgttgatcct acaaacttaa aacgtaatag cggcgcgcag gatggcgatt
     3841 gcatcatcgt tagtaaacca ataggggttg gcatccttag tgcaggctta aagaaagagc
     3901 gtctatcacc atcggggtac gagcaaatga ttgctctcac cacccagctt aataaaccag
     3961 gggtggactt agccaagatg actggtgtac atgccttaac cgatgtgacc ggttttgggt
     4021 tagcagggca cttacttgag atgggtcgcg gtgcacattt acgggccgag cttcggtggg
     4081 atcaaatacc gcttgtcacc gaggcggtag agcttgcaaa ggctgatatc tatacaggcg
     4141 catcgactcg taattggcaa gcgtatggtc atgagatcaa tctgtcagcc accatgcagg
     4201 aatggcaaca aaacctcatt acagatccgc aaacgagtgg gggcttgctc atttcttgtg
     4261 ctccacaggc cgagcaggaa gtaatccaac ttttgcagca atctggcttt atgcaggcta
     4321 aaaccattgg ccgatttact gctggcaagg gccttggcct ggtctaaact aagaaacagc
     4381 aaacaaatta caatcaaggt agttatttct agggagcgat aatgagtaat cagaatctag
     4441 cagcatgctt tcaggcattc attaagaata tgatgcagtc ccccacgaag cgtggcgagt
     4501 ggtttttgca gttaatgatt gagaagcgtg atcagcgtct tagcaaaaaa gtatattaat
     4561 ttgttttggt cttgatgtga tctgttcatc aagaaatcgt ataggcatac acctcggtgt
     4621 gtgccttttt cttttctcga gtctcgccaa ttctttagag aatgccgatg tggtgggtga
     4681 gggtcaaatc cgttacttaa agaatcggcc agatcctggc gcgtacacct acgagtctaa
     4741 ggtgacgatc agtaaagcta gcttagagtc tggcttggtc gagatccaga cctgccataa
     4801 gcaattagat cctatccgca aggtcgtcat tctctttaat aaagaccgaa ttaaagatat
     4861 tcaggtgcgc agcctcgatg gaattgcaat ggctgaggtg cgtgacaatc gggtcacatt
     4921 aagcgaggta caaaaaggtg gctcgatttg tattgatttg caatcacgcg cattagattc
     4981 tttagggaat ggaatgtatc agctcaatgc cggacctcta atgcgtcgtt tatttgatgg
     5041 ctatttaccc atgagcgcca atatgaagtt ctcatggcct aatcagctct tagcggtcga
     5101 ggagaccaaa ccccatagtc aggatggtgt tcgcatccaa caatcgcaag acgggcttga
     5161 gatggatctt gtattcgcag gcaggatgac cgcacagatt ctattaaagc gtacccaata
     5221 aataattaga gtttttcaca ccggtattgc tttgtatcaa tggtcaagag agattggctc
     5281 ggaatcaatt gcgccatcga gtttttagtc tctgctttac aggaacgatc aaaataactt
     5341 actagaccta gactgccaca gtaactcagc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnttggcca tctgcatagc agttccatgt gctgtagtca gcctggccgc aggcactgag
     5461 agccatacca atactcaaaa tgtaaagaat gcgcatttca gggacaatgg atcattattc
     5521 atcttacgag tgtatctgta aaacgtatgc gccatgcgct cttaccccta actctttttt
     5581 atgacggcgc ctgtcctttg tgtcaggctg agattattta tctatcttcc cgcaatcagc
     5641 agggattgtt gcgctttgtg gatatcaatg gtccagagta tgacccccaa gtagtaggcg
     5701 ttagttgcga acaagcactc gcacggatgt atggacaaat ggaagggcgg gaagcgattc
     5761 atggagtacc cgtctttgca gaagcctatc gtcgcgctaa cctacctgta ttggcttggt
     5821 tgttctcgca acctttatta agaccgatct ggaacctctc gtatcgattc tttgcaaaat
     5881 atcggcatac gatctccgga ctgatcggac cattctttct gcgactggca aaacgagtaa
     5941 gccattcaaa atcaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgaa gataccaacc
     6061 acttcgagca gtgcggatca tttctttgcc aacagtgcca actcctgttc gagcagtgaa
     6121 cttatagcag ggggtcctta acttcaccac cacgcattca acaccaccca tttgccaacg
     6181 atcaccaaca aatacagaat cttctagtaa gccactaatt gtgaggttct caccaaaata
     6241 gccatgcggg aggtccattg cggtcttctt agcctcgcta agataattag tccagtgagg
     6301 atagtgctca atggggtagg cgtatagagc tttttcaata ccaccatggg ccagatgatc
     6361 agcttgctca tcacttgcaa taccacttgc ggtaatttca atgggacaag gctttgcaag
     6421 agtgctgata ggagatttct tgatgcccga catcactgag cgtaattcat gatcctcgcg
     6481 gacccaaagt ggagctacga gaccggctga gatagtttca atacgaggca tcatttactg
     6541 ggcttactaa aatgtagatc tgcacaggga tccttgcgct caatgggctt gcttagccaa
     6601 acgtaatcat agcgatctcg atccttggca agattctttg agtcaagacc atcatcttct
     6661 ataaaggcaa tattgaccac cgatcgatga ggagcaatcg ctttgagaac ctggggtact
     6721 ccgtaatcct tacgaatatg tccattgcca gccaaaagaa ttccagaggg gcgctcgagt
     6781 aaagcctgtg ccatcgaaat atccgttaag cgctgaacca tcatcatggg ttttaaatag
     6841 gtgggcggta atttgccaca gtgaccatca atgagatctt gctccagtgc agacattgct
     6901 gatccggata gcgtgctttt ttggtagtcg gtcttgaact gcacaggaat cggattaggc
     6961 tctcctgaga aaatggcttt cgaaatcgag cgatctagat tgcttccaaa gattgggcgt
     7021 tgactaatac gtaattgctc aaaaagaggg ctgtagattg accagggcca agccttactg
     7081 ttaaagccgg ctgcatgcag agagtctagg gtcgaatctg taaaccgaac ttctttacca
     7141 gcaagtagat attccacaat caccgcttgc ttaggtttgt taagtcgcag aatgaactca
     7201 ccgcgtaatt ggtgatgact tgggttgtca tgaaactctc ccagcaatat gtaatccact
     7261 tgatccagtt tctgaatcaa ttccgccact gagagagtct tctgtgttcg agtatctagg
     7321 ataggacgct gaggatctac tttgccagcc gatgtctgac cgtacgnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntatct aatttgcggc
     7441 ttagggggag tgcccgattg ctttaattct ctaaatatga aacagtagca gatgtagctt
     7501 aaagagtaat tttgagaaac aaatactaat attaataata atctctgatc aacgcatcgc
     7561 agtgatttac tgagttgatt tgtatttatg taccgaataa caaaaattga tacagcaaag
     7621 gaattggtgt ttgtcgccga tgagcctcgt ggcaagtcgc acgaattcta ttttccaaaa
     7681 ttaagtgatg cagttttcgt tgaagagtac acgctcaagc tcatgagcag tgacggacgc
     7741 tacaaactcg tatctttact agcttattaa tgccgtgcgg tgttgtttgg cttgagactg
     7801 gattgcttgc tttgtctcat cggtcatacg gttgagatta cgaacttgta ataccatgcg
     7861 ggggttcttg ctcatgcttg actcatggcg catcaaaaaa tcccaataga gggtcgtcat
     7921 tggacaggca tccttgccat gggattgatc aggcttgtaa atacaggtct tgcagtaatt
     7981 actcatgcga tcaatgtact taccggttgc gatatagggc ttactggcca tgagaccacc
     8041 atcggcaaat tgactcatac cgatgacgtt tggaagttca acccactcga cagcatccac
     8101 atacatactt aaataccaag catgtacctc tttgggattg acccctagta gaagggtgta
     8161 caaaccagtg accattaagc gctggatatg gtgtgcgtat cctgtactca aactttgtgt
     8221 aatggtttgt tgcaggcatt gcatcgtagt cttgccatcc caaaagaatg ctggcagtgg
     8281 ggcgcttgca ttgagtgcat tttgctcaag atagctaggc atctgggtcc aataaatacc
     8341 gcggacgtac tcacgccaac ctaatatttg tcgaatgaag ccctctactg ccgcgatcgg
     8401 agctcggccc tgtgtgaact ctatctgggc tgcagcaacc acctcttggg cagagagcaa
     8461 cttcaggttg agggagctag caatatgcgc atgaaataac cacgtttcgt tggtccacat
     8521 cgcatcttga aactgaccat acagtggcaa acgattcttc ataaaatcag cgagtactaa
     8581 gagtgcatca gcacgcttta caggccaccc aaatccttcg nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnngc tttactaaag gcgctacgat tatcgtggtc gtagttccat
     8761 tcgccgccaa cgggttgatc gccctccatc aagatggcat gtttcttacg caactcccga
     8821 taccaatact ctaggcggat ttgcttacgg cctttgcgat gttgggcaaa gtcacgaacg
     8881 gtggaaaaga aatactcatc ctcccgaatc tctagcggta tgttcgtctg tttggcaacg
     8941 cctttaatct tctcaagtac gcgccagtca cccggagcgg tcatgatgag agtttgtggt
     9001 ttgagggtct tgatggcacg aagtaactca ccgtcaagag attgagtgtt gtcagaatct
     9061 tctaaggcac tgtagtgcaa ggggacctta gcctcctcca attgtttagc aaaatgccgc
     9121 atggcactaa taaatagtgc aatccgttgc ttggaggacc aaatatgcgt agattcctga
     9181 tgaacctcgg ccatccaaac gcaatcacgc ttgggatcaa agccagtaaa ggcgctggaa
     9241 tctgcattga gttggtcgcc aagcaccaca atcaggtggc gggtagaggt gggcttgggc
     9301 attgaattta tataaatgat ttatcccatt atcgaggaag tcaagaaagt gtgtagctct
     9361 ccataaattg ggatgtgcag aaaatagcta ataaattcaa tgactttctg actcatacca
     9421 aagaagtctt ggggcccacg tggcgacgct cgaattaggc aaaaacccga gtgctctttg
     9481 gggtaattcg tttatgatta atgaggcttt ttattaaaaa gactgttgaa agtctgaact
     9541 aaaaataatc atcaatgagg agatcctaat ggcaaagttg accatcaatg gaaaagtacg
     9601 aactgtagat gtagatccaa ataccccctt actttgggcg attcgtgagc aagttggctt
     9661 aacaggtacc aaatacggtt gcggtattgc gcaatgtggt tcctgcacag tattgctcga
     9721 tggagcagca attcgttcat gtgctttgcc agtgagcgca gccgagggta agaaaattga
     9781 aaccgttgag agcctctccg ttggtggcaa attaaataaa ttacagcaag catgggttga
     9841 taaacaggca ccgcaatgcg gctactgcca aaccggcatg attatggctg ctactgcctt
     9901 gctcagaaaa aatcccaagc ccagcgatgc cgatattgat gctgccgtaa ccaatatctg
     9961 ccgttgtgga accttccaag aagtgcgtgc cgcaatccat gcagcagcga aggcataagg
    10021 agaaaatcat gaatgcacgc gtacctaatt tatcccgtcg ccaatttatt gttggcacat
    10081 ctgcaattac aacgggctta gccattggtc tagagtttcc cggcgtcatt ggaactgctc
    10141 atgcacaagc tgctgataaa gccagcgccc ctgagattgg tgtttgggtt gtaattcagc
    10201 caaacgacac cgttgtgatt cgggtcgttc gctccgagat gggccagggc actattactg
    10261 gcttagctca attggttgct gaagagctag agtgtgattg gaaaaaaatt accatcgatt
    10321 acccaacacc aggtgagagc ctaaaacgca agggtgtttg gggatcattc tccaccggcg
    10381 gtagccgcgg tattcgtacc tcagaagact atgtacgcaa gggcggtgcg gctgcccgca
    10441 tcatgttggt tcaggctgct gctaatcagt ggggtgttcc tgcagtcgaa tgcttggcag
    10501 tcaatagcat cattacacat caggctagtg gtcgctccgt tagctttggc aaggttgcag
    10561 ccgcagctgc taagttaccc gtacccgatg caaagggtat tgaactaaaa gatccgaagc
    10621 gttggaaggt aatcggtaag ccgatggatc gtttggatag tttggctgat aaggtcaccg
    10681 gtaagcaggt ctatgcgatc gacctcaaga tgcccggcat gttgaacgca accattaagg
    10741 acagccccgt atttggtggc aagctcaaaa gctttgatgc tagcaaagta gctggcatga
    10801 agggtgtcaa aaaggttgtg caggttggtg attcagcggt tgccgtgatt gctgacacat
    10861 tttggcaagc aaaaacagcg atggatcgtt tgccaatcgt atgggatgaa ggccctaatg
    10921 caaatgtgca acaagccgat attctgaagc gtttagaaga aggcttaaaa gcagagcaaa
    10981 cctttgtagg taatgcgaac ggtgattaca agaaggtcgc tgcaga
//
LOCUS       DDPQ01000006            5061 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5302,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000006 DDPQ01000000
VERSION     DDPQ01000006.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5302"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1533..1545
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1353 a         1067 c         1283 g         1341 t
ORIGIN      
        1 gatgccatca tcaatcaaaa gggtaagggt gtttactgta tttatgtagc gatcggtcaa
       61 aaggcttcca ccattgctaa cgtggtccgt aagctaaccg agcttggcgc aatggaatac
      121 acnntgcacc atgggcgaat acttccgcga tcgcggtgaa gatgccttga ttatttatga
      181 tgatttaaca aagcaggcgg ttgcttatcg tcaaatctcc ttattgttgc gtcgcccacc
      241 aggtcgcgag gcatatccgg gcgacgtgtt ttatttgcac tcacgccttc ttgagcgtgc
      301 tgcgcgagtc aacacagatt acgttgagaa atttacgaat ggtgcagtga agggcaagac
      361 cggatcatta accgccttgc ctgtgatcga gacgcaggcg ggagacgtat ccgcattcgt
      421 acccacaaac gtaatttcga ttactgacgg acagatcttt ctggaaaccg acttatttaa
      481 tgcaggcgtt cgtccagcga ttaatgccgg catctccgta tcacgtgtgg gcggtgcggc
      541 acaaacaaag gttattaaga agctttcggg cggtattcgt accgaccttg cccagtatcg
      601 tgaacttgca gcatttgcac agtttgcgtc tgacctggat gaggcaacac gtaagcagtt
      661 agagcgtggt aagcgcgtta ctgaactttt gaagcagcca cagtatattc cgatgcaagt
      721 gtggcaatta gctgcctcac tgtatgcagt gaacaacggc ttctttgatg atttggaaat
      781 caagcaaatt ctgccctttg aaaaaggcct acatgagcac cttaaatcaa agtttgcaga
      841 tttagttggg cgcattgaag atacgaagga cctaagcaaa gatgacgaaa ccgccttgcg
      901 cgcagcaatc gaagacttta aacgttcggg ctctttctaa tttaggcgat catggcaggt
      961 acaaaagaaa tacgcagcaa gatcaagagc gttcaaaata cgcgcaagat cacgaaagcg
     1021 atggaaatgg tggccgcatc gaagatgcgt cgtgcgcaag aacgtatgcg ctctgctaga
     1081 ccctactcag aaaaaattaa agcaattgcg tcgcatttag caaaagctaa tccagagtat
     1141 cggcctgcct atatggtggt gcgtgagctg aaaaaggctg gcgtcatttt agttagcact
     1201 gataagggat tgtgtggcgg cttaaatacc aacattttgc gtttaattac gaatcaggtt
     1261 cgcgagttta aggataagaa tgtatccatc gaatttactg cgataggttc aaaaggctta
     1321 cagttcttga atcgagcaaa ggctaagctt ctgtcgcagt cggtgcattt gggcgacacc
     1381 ccgcatatgg atgtgttgat tggtgcgatc gctgcccagc tcactgcgtt tgagcgtggt
     1441 gaggttgatg cggtctattt agcttatacg cgcttcatta atacgatgaa acaagagcct
     1501 gttttagaga aactcttgcc gcttgagtcc tcnnnnnnnn nnnnnggtgg ccatggctgg
     1561 gattacatct atgagccaga tgccgaatcg gttttgaatg gattattaaa acgttacgta
     1621 gaagctctta tttatcaggc tgtggcagag aacatggctt ctgagcaatc ggctcgaatg
     1681 gtggcaatga aagccgcctc ggacaatgcc aaaaacgtga ttggtgagct acagttgatt
     1741 tataacaaaa ctagacaagc agcgattaca aaagagttat ctgagattgt ggctggagcc
     1801 gcagcggttt aattgtaaaa aatttaaagt ttaaatagcg gagaaacata atgagcaacg
     1861 gaaatatcgt tcaatgtatt ggtgcagtgg tggatattaa gtttcctcga aatgaaatgc
     1921 ccaacattta tgatgccttg actctagtag agagcaagga agcctcattt gcagaaaaag
     1981 gcttaacctt tgaggtccaa cagcaaattg gagacggcgt agttcgcgcg attgcaatgg
     2041 gttcaagcga cggattgcga cgcggcatgg aagtgaaagc aagtggtgcc ggcatcaagg
     2101 tcccggtggg ccccgcgacc ttgggtcgaa ttatggatgt gcttggccga ccaattgacg
     2161 atgctggccc aatagcaacg caagatcgcc gagccataca tcagcttgcc ccaaaatttg
     2221 atgagctttc cccgtcggtt gatttgctag aaacaggcat taaggtgatc gatttggtct
     2281 gcccatttgc taagggcggc aaggttggcc tgttcggtgg tgctggtgtg ggcaagacag
     2341 tcaatatgat ggagctcatc aataacattg ctaaacagca ctctggatta tccgtatttg
     2401 caggcgttgg agagcgtacc cgtgaaggca atgactttta tcatgagatg aaagagtcta
     2461 atgttgttga taaagtagcc atggtatttg gtcagatgaa tgagccacct ggaaaccgtt
     2521 tgcgcgtagc cttgacaggt ttaacaatgg ctgaggcttt ccgcgatgag ggccgagaca
     2581 ttctgttctt cgtcgacaac atttatcgtt atacccttgc gggcaccgaa gtgtctgcat
     2641 tattaggccg tatgccttca tccgttgggt atcaaccaac cttggctgaa gaaatgggaa
     2701 agcttcaaga gcgtattact tcgacgaaga ccggctcgat cacttctatt caggcagttt
     2761 atgttccggc cgacgactta acagacccct caccagcaac tacttttgct cacttagact
     2821 ccaccgtagt tttatcgcgt gatgttgctg cgttgggtat ttacccagcg gttgatcctt
     2881 tggactcaac aagccgtcag cttgatccac aagtggtcgg taccgagcat tatgaggttg
     2941 ctcgcggagt gcagattaca ttgcaaaaat ataaagagtt acgcgacatt attgcaattt
     3001 taggtatgga tgagctttca ccagatgaca aattagcggt agctcgtgct cgtaagattc
     3061 agcgcttctt atcgcaaccg ttccacgttg ctgaggtatt tacaggctcc cctggtaaat
     3121 atgtaccact aaaggaaact attcgcgggt tcaagatgat tattagtgga gagctagatc
     3181 atctaccaga gcaggccttt tannatggtt ggttcgattg atgaggcaat tgaaaaggcg
     3241 aaaaaactgt aatggcaaca atccacgttg atgttgtaag tgcagagcgg tcgattttta
     3301 gtggcgaggc aaaatttgtt gctttgccag gagagtctgg cgagcttggc atattgcatg
     3361 ggcatactcc gctgattacg cgaataaggc ctggtgcagt ccggattgaa aaagcagacg
     3421 gtgatgaaga gtttgtgttc gtggccgggg gttatctaga ggttcaaccg gacagtgtga
     3481 ttgtgttggc agatactgca atccgtggtc atgaccttga tgaggcgaaa gctgttgagg
     3541 ctaaaaagcg tgccgaggag gctatgcaaa atcgtgctgg tgattttgat attgctcacg
     3601 ctcaatcaga gtttgcgatg gctgcggccc agttggctgc aatatcgcgc ctacgccgta
     3661 aaaaataatt aaacaattgt taacaaacga tcgattctta aaggcttgct taggcgaaaa
     3721 aacagaccgc acgcctctgt ggttaatgcg tcaggcaggg cgctacctac ccgaatacaa
     3781 tgcaacacgt gcaaaagcgg gtagtttttt aggcttagcc aaaaatcctg cctatgcgac
     3841 agaagtaaca ttgcaaccct tggatcgtta tccgctggat gcggcaattt tgttttctga
     3901 tattttgaca atacccgatg ccatgggttt gggactgcag tttgctgctg gcgagggccc
     3961 cagttttcag catcccttgc gcagtgagga cgaagttaaa aaactgaggc cagctgatct
     4021 gcaacaactt cagtatgtct ttgatgcggt cgcacaaata cgacaggcgc ttattcaaga
     4081 cggcaaacaa cgcgtaccct tgatcgggtt ctccggaagc ccttggactc tagcctgtta
     4141 catgatcgat ggttcaggtt cagatgattt tcgtcatgct aaaacgatga tgtttaatcg
     4201 ccccgatttg cttaaacata tattggatat caatgcccaa tcggtagctg cttatttagg
     4261 tgagcagttg cgctcggggg ctcaagcact tatgattttt gatacctggg ggggattgtt
     4321 accagatggt tggtatcaaa gaatttcttt ggccagcatg cgtagcgtga ttgatcaatt
     4381 acctcgggaa gttaatgggc aaaaagtccc tattattatt tttacaaagg gcggcggttt
     4441 atggctggag gacatggcag acgtgggtgc cgatgtgatc gggcttgatt ggacgatgtc
     4501 cattgccaag gcgcgcaccc tattggtttc taaaaataag ccactagcgc ttcaaggtaa
     4561 ctttgaccca atagcgcttt ttgccaaacc cgaacaaatt actcaagagg ctattggcat
     4621 actggattcg ttggcaaaag ccccggctct aaagtctggg ctccatccct tggatggcca
     4681 tatctttaat ttgggccacg gcatatctca atttaccaat ccagacgccg ttacagtttt
     4741 ggctaaagcc gttctggagc attctgccca actcagaaaa gtagtctaaa tcgggataaa
     4801 tccctataaa tcaaggcttt taaggggtac ttatacacat ggataggcca tgatcctgat
     4861 ttcatgggga tttaagacaa agcaaatgta ttagagcctt aaaatttacc aagccattga
     4921 tttaattcac tttttattaa aagcgcatat ttatgaagag cccaatgcat ttatgcgatt
     4981 atcttcagga cccccataaa taggggttat ccacaaagtt atccacagac cattaaagta
     5041 aacgcccact tgaataatct t
//
LOCUS       DDPQ01000007           19860 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2192,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000007 DDPQ01000000
VERSION     DDPQ01000007.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 19860)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19860)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19860
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2192"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    8555..8605
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13370..13436
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13915..13957
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14903..14977
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5269 a         5045 c         4166 g         5144 t
ORIGIN      
        1 aggcactcaa aagccagcaa aggagcagca aaagcctaag aaacaggaaa agaaagtgaa
       61 aaagcttaag aagactgaga atcctgcaaa ctgaatttat gcaggaaata cgcctgtcga
      121 taaataacga tcaccgcgat cacacacaat aaagacaatc gttgcgttct caactgttcg
      181 tgcgacacgc aatgcaatca ccaatgcgcc agctgctgag attccacaga aaatcccttc
      241 ctctttagcc atacgacgtg ccatctcttc agcttcagcc tgggtcacat actcaatacg
      301 atctacccgc tccccttgat agattttagg aaggtatgct ggagcccatt tacgaatccc
      361 cggaatttgg gaaccatcct ccggctgccc gccaataatt tgtatctcgg gattcattga
      421 ctttaggtaa gtggagaccc cagtaatggt tcccgtcgtt cccatcgccg acacaaaatg
      481 agtgatttgc ccatgtgtat cacgccagat ctcggggccg gtggtttcaa tatgagcgcg
      541 gggattatct gtattagcaa attgatccag aagtttgccg cgcccttctt tttgtaattg
      601 ctcggcataa tcacgggcta actccatgcc tccagaacta gaggtcaaaa ttaactcggc
      661 cccataagcc gccatactct gcctacgctc tagactttga ttctcaggca tgaccaaaat
      721 aattttgtaa cctaacattg cgccaaccat ggcgattgca atccctgtat taccactggt
      781 ggcctcaatt aaggtatcac caggcttaat gtcaccgcgc tcttgtgcat gcaagatcat
      841 tgaaagtgcg gggcggtctt tcaccgaccc cgcaggatta ttaccttcta atttgcccag
      901 aatgatattg ccacggcgct cattctccat tcctggaata cgctgtaagc gaaccaaggg
      961 tgtattccca atggtttgag aaatggttgg atatagggaa tgagcagggt ttgattgagt
     1021 cataaaccca ttttagccac aagcaaataa atcggtaaat ttactcaaaa ccgtttacgc
     1081 gtactcggct tctcggtctc agcctgaggc gctttagctt ggctcttgga acttctccgg
     1141 tttggtcgtg actctgtacg cgcattactt ttggtctcac gatacccgct ctgtccctca
     1201 atctttaaac cattctccat cagcttacca accgatcgct caccaatgcc gcgcacccgt
     1261 gaatgcaaat cataggaatc ataaaaggcg ccacccttct ctcgctccgc aattattgct
     1321 ttggcctttg atggccctaa accacgaata ctttcgagct ctgactggct agcagtgttc
     1381 acattaatag gacttgaatg cgcatagttc atacaaagtg gcgcagcaag cgctaacgca
     1441 atcatccaaa tagctgattt tttggataag ctatttttag atgtattgat aagtgctgat
     1501 aaacccatat tttctccttg agtgttaacg aaattcccat gacaaaaaga catggtcttc
     1561 ctcaacgcta ggaaatggat tgtgttgact aaaaaaaggg tttagagagg attggccaga
     1621 aaatcagaat tcgctgacag ccaagcgatg tatcggctga caccctcctc aacatttagg
     1681 aagtctgctg aataacccgc agtccgcaat tgagtaaggt cggcctgggt aaaacattga
     1741 tacttaccgc gtaagtcatc cgggaatgga atgtactgaa tcagacccgc ttttactaac
     1801 tctgttaaag gtaatgcatt ctcacctttt aatttacgca ttgcattgac agtggcaatg
     1861 gctacatcat taaagggttg cgctcttccc gttcctaaat taaagatacc gctcttctca
     1921 ggatgatcaa gaaagaacat attgaccttt acgacatcct caactgagac aaagtctcga
     1981 gcctgctgac ctgctgcata acctccatac tcaccaaaga gttttacagt ttgattggtc
     2041 ttaaattggt gatagtgatg aaatgctacg gaagccatgc gacccttgtg agattcacgt
     2101 gggccataga cattgaaata ccgaaaacca acaacttggg cggttggcgc tctctcagca
     2161 aaacgagcac gcatatactg atcaaataaa aatttagagt agccgtaaat attcaaaggc
     2221 ttctcatgtt gtcgttgctc cacaaaaaca tccgatccgc cataggttgc cgcagatgag
     2281 gcatataaaa atggaacttt ttgtgtagtg caaatctcat atagattgac ggagtactga
     2341 taattattat tcatcataaa aacaccatcc gtttccatgg tgtcagaaca ggccccctca
     2401 tgaaagactg cctttacctt cccaaaccag ccctcatcaa atccttctaa gaaatcgttt
     2461 ttgtcgatgt aatcagcaat atcaagatca gctaaatttc gatatttatc agcaggacgc
     2521 aaatcgtcaa cagcgataat atttttttcg ccacgagcat tcagtgcccg cacaatattt
     2581 gcgcctacaa aaccagctgc gccagttacg atatatgtca ctgaagctcc tctgcggaaa
     2641 cggttgctgt acccagctta cccaccacta taccgcctgc acgatttgct aatgccatcg
     2701 cacgctcaag tggccaaccg gctgccatag ctactgccag ggtagcgatc acggtatctc
     2761 cagcacccga gacatcaaat acctcacgcg cctgagcgcg aacatgttcc gtaccctttt
     2821 cggtaaagag agtcatgccc tcttccgagc gggtcaatag caatgcacta agctctagtt
     2881 cttgacgcaa ggactgagcc ctttttgtaa gatcagcttc atcactccat tgcccaatca
     2941 cctgacgcaa ctcactccga ttgggagtta aaaccgtagc gcctttgtat ttgctataag
     3001 catcaccctt aggatcgact aaaactagtt tcttattcgc cttcgcaaga gcaatcatgg
     3061 cggacacttg ctctaatgcg cctttgccat aatcagacaa aatgacgaca tctacttggg
     3121 gcagcaaact ctcaaaacgt tttagtttct catccaagga gttttgactg ggcgtctctt
     3181 caaagtcgag acgaatgagc tgctgctgtc tagcaattac tcgcagctta actgtagtag
     3241 gtaccttact atcagcttgt aattgacttt ggacaccact ttcttggagt aactgctcaa
     3301 cccgcttacc agcctcatct tgaccaataa caccgaggat cgtggttttc gcacccaagg
     3361 cagaggcatt gcgagctacg ttggcagcac cacctaaacg ttcatcaatt tttgcgacct
     3421 ggaccactgg caccggggct tcgggtgaga tgcgtccagt atctccaaac caataacgat
     3481 ctagcatgac atcacccacc actagcaagc gcgtcttact aaattgattg cgatcggctt
     3541 tttctatagt caatttgggc tctttattaa ttcaacatcc taattatgcc gaccaatgcc
     3601 ttgatactca aaaccgagct ctttcatagt ttggggctca tataaattac gtccatcaaa
     3661 aatgatgggg gttttcatct tctgttttag tatctcaaaa tcagggcttc taaacgcttt
     3721 ccactcggtc acaataatca gagcgctagc ttggtccaga gcatcttctg gagcctttac
     3781 catctttact tgatccaaaa acttagggtg cgacttaaag tcaagctcca gacagcgctg
     3841 cgcctctggc atggctacag gatcgtgcgc caaaatatta gcgcctcgct tgaccaactc
     3901 agagatgatc aaacgactgg gtgcctcacg catatcatcg gtattcggtt taaatgccaa
     3961 accccaaaga gcaaattggt ggccctttag gttctccccg aatcgcttaa cgatcttctc
     4021 aatcaggatc gttttttgag cttgattcac agactcaaca gcttccagaa tctttagatc
     4081 acgtccatat tggtgtgcag tttgagaaag tgccgaaaca tccttcggaa aacaagagcc
     4141 gccatatcct gtgcctgcat ataaaaaacc ataaccaatg cgagagtctg aaccaatccc
     4201 ttggcgaact gcctcaatat cagcgccaac ttcatctgcc aaattggcta actcattcat
     4261 aaatgagatt cgggtggcta gcatggcatt cgcagcatac tttgttaact ctgcactttt
     4321 tacatccata taataagtac gctcatgatg acgattgaag ggcgcataaa gcttgcgcat
     4381 catttccttg gcccgtaatc ccgctggatc tgcattggtg ccaatcacga tacgatcagg
     4441 gcgcataaag tcttcgactg cagccccttc ttttaaaaac tcaggatttg aaaccactga
     4501 acacaaagca ggatcaagat ttcgtttctt aagctcttct tgtatagcag tggttacctt
     4561 ttctgcagta ccaaccggca cagtagattt atccacaata acctttgggc tagtcatgtg
     4621 cttaccaata ttgcgagcgg cagcgatcac atattgcaaa tcagcagaac catcttcatc
     4681 ggggggtgtg cctaccgcaa taaattggat atcgccatgc gccactgcgg ctgctacatc
     4741 ggtagaaaac tgaatacgtc ccgcagcacg attacgggcg atcatctctt gcagaccagg
     4801 ctcataaatg gggacgccgc cagagttcaa gatatcaatc tttttttgat caatatctaa
     4861 acaaaaaatc gtattaccaa gctctgctaa gcaagctcca gtaacaagcc ccacatatcc
     4921 acttccaaca atcgttacct tcaattaagc tccttaaaac cctcgtgaat tcattcctta
     4981 actaccgctt attattccta acaattttga atcatttctt acatagacgg gccacttggt
     5041 acagcgcctt cactccgcct tggagaatac gcttcccaat gattgcagcc tgggcactgc
     5101 caataaaaac gtcgagctct aaagccacaa ttgccgcagg tataacgcgc tagggctgtc
     5161 gtgcgctgtt tgagtaaacc caaggtggat ttcaaatcag cgactcggct ttcgggcccg
     5221 gaggcctcgg caagtactaa acgcgtctct gctaattttg agagtgcgct caaggtaggt
     5281 gaatgttgca tcacctctac tagcattttc tcagcggctt gtgcgccacg tatttgcata
     5341 acatgcctat gcgcaatatc taacaactct ccaatcgctt gggtctttaa tagagccaac
     5401 agttgatcta agcctgaatc ggcctgactc actgcagaat gtgcagccat ccagcgatca
     5461 gccactaaat gcataaaagc aggactctct ttggcaacaa ccccccaaac ctcgatagcc
     5521 tgtaatggac gttccattgc cagaaagtaa tcgccttgca aaataagagc gcgggcttga
     5581 ttcgggatcg cttgtaaggc atggttaata cttgtttcag cctctgccat gtctttacgt
     5641 cttaaggctt cctgtgcgat ctcacagtaa aactgggcaa tctctagttg atggcttttg
     5701 tcttgcaaac cttggagttc tgtagccgca atgattgctt ttttccaatc cttctcaacc
     5761 tgatacatct ccaacaaact ctcttttgct ggaatggcat atttaccctg ccccacctga
     5821 tttaaagacg cctctgcgcg atccaataga cctgcacgta agaagtctcg gcctaactca
     5881 taggccgcat gatcccgatc acgcggctta atatcatcac gatttgctaa atgttgatgt
     5941 acccgaattg cacgttcggt ctcgccacgg cggcgaaaca aattgcctag cgagaagtgc
     6001 aaatcgactg tctctggatc aagttgtgcg accttgacca aggtttcaat ggcctgatcc
     6061 ggctgctcat tgagcaaaag gtttaaacct ttaaaggtgg aacgctgttg ctttaagcgc
     6121 tcgcgctcat cttgacgcgc ctctaaacgc aaatcccagc gcgaagcaat ccaacccaaa
     6181 ccaaaaaaga taggtattag gagtaaccaa ccggtttcta tttgaatcat gttcagtggc
     6241 ttttagaaaa gaacttattt ttagatctgt agcagacctg ccaccgcggg actaggcacc
     6301 cgaactccct ttgtctgtaa ctaatttttg attaagtggt ttgtaatcca ctcgctcacg
     6361 tagctcttta cctggcttga aatgaggtac ccgcttttca gggattaata ctttctcacc
     6421 ggacttggga ttgcgccccg tacgagcagg acgatggtgc agaacaaaac taccaacgcc
     6481 acgtaactca atacgcttac cttcagccaa tgcttgggtc atggtgtcga gtaatgtttt
     6541 gacggccaac tcaacgtcac gaggcaagag ttgcggaaac tgctccgcta aactctcgac
     6601 taactcggaa cgggtaatgg cttgatgctc ttgatctgac atagtctaat cagtccgcca
     6661 ctctaagagt ggcggaatca ccttagcttt ggttatccat tttcgctttt aacaaggcac
     6721 ctaaattcgt tgtaccagag ccagcatcac tctgtaactt attcatcgta tcttgttgat
     6781 cagcgctatc ttttgcctta atcgacaaat taatagcgcg tgacttgcga tcaatattga
     6841 taatcatggc agacacagta tcaccctctt taagggcatt acgcgcatct tccacacgat
     6901 ccgttgagat ctcagaagcg cggagataac cttctacctc atccgccaaa tgaatcactg
     6961 cacccttcgc atccacactc ttcacagtac cagagactag gctacccttg tcgctagttg
     7021 atgtgtaatt attgaagggg tcgcctgaca tctgtttaat gccaagagaa atgcgctctt
     7081 tctcaacatc aatagccaag accactgcct cgagctcatc gcccttctta aacgctttaa
     7141 cagcctcttc gcctggctcg ttccaagaca ggtcagataa atgaaccagg ccatcaatac
     7201 cgccaggcaa accaatgaag acaccaaaat cagtaatcga tttaatggcg cccttaatct
     7261 tgtcgccctt ttgatggctg cgtgagaact cttcccaagg attggctttg cattgcttga
     7321 tacccaagct aatacgacgc ttgtcttcat caatatccaa gaccatcacc tcaacttcgg
     7381 tccccaaaga aacggcttta cttggagcaa cgttcttatt ggtccagtcc atttcagaaa
     7441 cgtgcaccaa accttcaatg ccactttcga tttcaacgaa tgcgccgtaa tcagtgaggt
     7501 tggttacctt accaaagaga cgtgtgttgg gtgggtaacg gcgtgcaata ccaacccatg
     7561 ggtcatcgcc taattgctta atacccaagg aaacacgatt cttctcttgg tcaaatttaa
     7621 gaatcttggc ggtcacctct tggccaacgg tcaacatctc actggggtga cgcacgcgac
     7681 gccatgccaa atcggtaatg tgcaagagtc catcgatacc accaaggtct acgaatgcgc
     7741 cgtaatcggt aatatttttg atggtgcctt gaacaacgct accctcttgg aggttagaca
     7801 tcatcttggc gcgctcttcg ccttggctag cttctacaac agcacgacga gagaggacaa
     7861 cgttattacg cttacgatct aatttaatga ccttgaactc catggtctta ccttcataag
     7921 gactggtgtc cttaatgggg cgagtatcga ccaaagatcc tgggaggaac gcacggatac
     7981 cgttaaccat aacggtcagg ccgcccttca ctttgccagt aacagtaccg gtcacgatct
     8041 ctgcgccttc aagcgctttc tcaaggttca tccatgaggc caaacgcttg gctttatcac
     8101 gagaaaggat ggtatcgccg taaccatttt ctagcgcatc gattgcgaca gaaacgaaat
     8161 caccagggtt tacttcgagc tcgccctggt cattttggaa ttcttcaaca ggaataaatg
     8221 cttcggactt tagtcccgca ttcacaacga caaaattatg atcaatacga acgacttcgg
     8281 ccgagataac ctggccggtt ttcatattgg atcgggttag ggattcttca aataatgctg
     8341 caaacgattc agacatgagt ttcacttttt gtgccgtcaa aaaaccgacg ggttaaatta
     8401 ataaaacctt agaaggcggg tataaaccgt agatgccaac taaagcgcca aaacaacaac
     8461 tagtgatgca attttgcata ccagtttaaa acctgcgtaa ctgcctgatc gaccgataaa
     8521 tccgaggtat ccagcaaata tgccccttcc acacnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnaaaag gacatccatt ctagcagaaa ttcccttagc
     8641 gatcaattgc ttatatcgac gctccgctct ggcttgtacg ctagctgtca aatagacctt
     8701 taaaaccgca tcggtaaaga tccggctggc catatcgcgg ccatcggcca ccaagccggg
     8761 gttttgtcta aattccctct gaagggtcaa taaagcgtcc ctaaccttgg ggtaaatagc
     8821 cagctgcgac gctctagtcc ctattttttc ctcccgaata agctcagtaa catccttacc
     8881 gttcagaaaa atcttgttgt ccttaaaaac aacctgcata cgggacgcaa tagcccccaa
     8941 acccgactca tcgtctactg aaataccagc ctcttcactt gcgaaagcaa ctagacgata
     9001 gagcgcccca ctatctaagt aatgaaaact cagttggtca gccacacgca tcgctacggt
     9061 tcctttaccc gaagcagtag ggccatcaat ggcaatgact gggggtggat tactcactgt
     9121 tctcatggaa gtcaattaat gcgtaatact tgctagctca gagaaatagt gggggaaggt
     9181 tttagcaacg caatgaggat cattaatctg aatttgcttg ggaccaaatg cgcacaatga
     9241 aaagcacatg gccatacgat gatcatcgta ggtatcaatt ccattgaccg gtgattgcca
     9301 atcttgctga cccaagggat gaatctcaat aaagtcgggg ccctcaataa cttgggctcc
     9361 taattttcgc aactcctttg ccatcgctgc aatgcggtcg gtttctttaa cacgccaact
     9421 cgcaatcttt tctagacggg ttttgccttt ggcaaagagt gccatcactg ccagtgtcat
     9481 cgcagcatcc ggtatagcag tgcaatcgat ggtgatgcca tttaactggc catgacgaac
     9541 gccactagtt tgtaaataat ggtcgccgcg ctcaaccaca gcacccattt tttcaatcgc
     9601 atctgcaaag gcaatgtcac cttgaatgct ggtcttaccc actcctaaaa cacgtaccgg
     9661 tccttgtcct aaaactccgg ccgccaagaa ataggatgct gatgaggcat cgccctcaac
     9721 atacaaagtc tgtgggctgg tatagacagc acccgcctca gtgggagcaa tcacaaaggt
     9781 atttctatct ggacagctca cctcaacacc aaaacgtgcc atgagcttcg tagtgatatc
     9841 aatgtatggt cgagaaatta attctccgag cacctcaatc cgaactgctt tgcgcgctat
     9901 taatggaaga gccatcaata aggctgttaa gaattggctc gatacatcac ccctgacctg
     9961 aatcgcctcc ggaatgctaa tcacactctg atgaatcaac aacggcggat accccacttc
    10021 cttttcgtaa tcaatttgtg cacccacttg ccgcagccca tcaaccaaat caccaattgg
    10081 gcgctcatgc attcggggta cgcccgataa acggtaattg ccctgctgca ttactaatgc
    10141 tgcagttaat ggacgtattg cggtcccagc attacccatg aataaatcag cttctttatt
    10201 tggaaataac ccgccacatc cgttcacaat gcaatcaaga ccatgatctt caagctggac
    10261 accaagttga cgtaaggcct tgcgcatcac ctgcgtatca tcggcatcca gtaattgctt
    10321 aagggttgtg gttcctttgg caagagcggc tagcaataga acacgatttg aaatactttt
    10381 agagcccggt aaagtaatct gaccttttgc tccctgaaat ggtccaatct gaattgctgg
    10441 actggccatt aagacgcatc ccacttttgt cgtgcctgac ttgccttttg aaaaaccttt
    10501 tcaagaccag ctgcgtcttc tttagcaatc atctcacgca aacgtccagt cacactcaag
    10561 tattggtcga gttctttgag gattgcttgc ttattcgcaa tacagatatc gcgccacatc
    10621 tctgggctcg atgcagcaat ccgtgtgaaa tctctaaacc ccgcgcccgc atgcccaagc
    10681 ttttgttccg catcctccga attaagcacg cttgccatca aagcatagga gaggatatgg
    10741 ggtaaatgag agaccgcggc atagatcgca tcatgctgta ctgctgatat ccgtttaata
    10801 tttgaaccca gtactttcca aaactcttct accagagcga catcggctgc gctattttct
    10861 tgcaactgac aaatgattgt ttgttttcct tgaaataaat cagcgcgagc agcagcagca
    10921 ccgtgctgcg ctcctcccgc gatcggatgt gcaggaacaa attgacagac ttttttacct
    10981 aagacttctt tggcagccaa aattacatcg ctcttggtac tgcctgcatc tgtaattaag
    11041 gtgttggatc ccaaatgggg ctctaattga gtaaagattg ctcgcatttg tgcaaccggt
    11101 acgcacaaga cgatccattg tgaagattgc acagcttcgg ctaaatcagc cacccgatca
    11161 atagcgccca tctttttggc ttgatctaaa ttggcgctac ttcgccccac accaatgact
    11221 tcgcgcacga cccccgcccc tttgagcgct aatcctaatg atgcgcctat taagccgaca
    11281 ccaacaatac tcacaacacc aaactgattt gtgctcatgc cagcaccttc tcatctttga
    11341 ggatttgaga aaaggcttcc aagaaggccg cgttttcttc cggcaatcca atcgaaatcc
    11401 taagccattg aggcaaacca taattagcaa ctggtctcac gatcacgcca cgcttaagta
    11461 actcttgatt aactcgtgcg ccggcctgat catcattgcc tactttgacg agaacaaaat
    11521 tacccgctga tggaataaat gcgagtttca tctgctcaaa tgcctttgtt aattgcgcat
    11581 agccagctct attcagctca tagcccttca ataagaacgc ttcatcttgc aatgccgcaa
    11641 tggctgcagc ttgagctaag ctattcacat taaacggttg acgaatccga ttgagcaaat
    11701 cagtgagagc aggttgagca atgccataac caatgcgtag gccagctaat ccataagcct
    11761 ttgaaaaact tcgcgatacc atcaaattag ggtgagcgcg aacccaagca atggcgtcat
    11821 agcgctgctc tggagataag tattcgttgt aggcttcatc caaaacgatc acaacatttt
    11881 cagggacttg ggtaataaat gattgaatag catcaggctg taaatagctg ccggttggat
    11941 tattgggatt cgcaataaat accaacttaa cttgatcgcc taaagaccga atgaccttta
    12001 acatcgctgg cagatcatgg ccatagttgg catccgcaga aacttcaatg gcctttgcgc
    12061 caatagcctg agtggccaag ggatagaccg caaaagcatg tcgagagaac accactgcat
    12121 ctcctgcatc cgcaacagct cgagcggtaa gctcaaggat gtcgttacta ccattaccga
    12181 gcgtaatcca atcctctgga actgcaaggc gcttcgatag aacttgcttt aactcaaagc
    12241 cattggagtc tgggtagcgc cctagatcgc tggccgcctt catcatcgcc tgtttagccg
    12301 attcgggcat tccaagagga ttttcattgg aggccaactt cacaatcttg ctctcgtcta
    12361 agccatattg acgggcaacc tcgctaatgg gcctgccacc tacataagga gcaattgctt
    12421 gcacatggtc taaaccaatt ttctttgcca ttgaattcag ttgaacgtaa taaagatgaa
    12481 cttagttttt agctctcggg taagagccaa gctttttata gaacgctgcg attgttttta
    12541 actcttcaat agctttggct acttttacat cgtccgcatg gccttcaata tcgatataaa
    12601 agtgatactc ccaagagccc ttgcgagctg gtcttgactc aaagcgggtc atcgagacgc
    12661 catgtttagc taatgggcct agcaaatgat gaacagcgcc aggttgattt gagaccgaca
    12721 acaccaaaga ggtttgatcc tgaccgcagg gttggcaagc atattggcct atcactacga
    12781 agcgagtgcg attatttgga tcatcctgaa tttgcgcagc aatactttgt agcccataag
    12841 caatttgagc aggatcccca gcgatagctg caatacttgt atcaacactt gccatacgag
    12901 cagcttctgc attgctacta accgcttggc gttgtaagtg agggacatgc acactcaacc
    12961 attgctgaca ctgggctaat gcttgcgcgt gagcacagac cgtcttgata ccatccaaac
    13021 ttccggtctt gcttaatagg tgatgacgaa tcggcaatac cacctcgccg ctaatttgta
    13081 gtgggctctc caacagcaag tcgagagtgc gggagatggc accctcgcta gagttctcca
    13141 ctggcactac cccaaaattt gcagcgccct tctcaaccga tttaaaaact tcatccaaac
    13201 taacgcacgg taagccttgg acagattgac caaaatactg gtgcgccgcc agctctgaaa
    13261 aggtgcctac gggtcctaaa taggcaactg tttgtctgga ttccaacgct ctgcacgcag
    13321 acataatctc acgccaaatc gaagcaatcc catctgccag caaaggcccn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtca
    13441 aaagctccaa gagttgagta tcaatctcat caatcttggc acgcaaggga gttaaacgtt
    13501 gttcatcctt tgacattagg cccgcctctc aaattcctgc atgaacgcca gcaaggcttc
    13561 cactccagca atgggcatcg cgttgtagat actcgcacgc atgccacctg cggctttgtg
    13621 ccctcgcagg gcagccaatc ctgcctcatt ggactgtgcc aaaaactctg cattcaaacc
    13681 ttcatctttc agaaaaaaag tgacattcgt tctagatcgg caatttttat taacgcgact
    13741 ctcataaaga gagctttgat caataaagtc atacaacaaa gatgatttga cctgactgcg
    13801 gcgttccatc tctttaattc cgccctgacg cttaatccac ttaaatacta agcctgaaac
    13861 gtaaattggg aaagtgggtg gggtattaaa catcgactga ttagcaactt ccttnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnact ccacattggc gcacatccat
    13981 ttttttcgag aggatattgc tagaaatgtc agcaaccaag ggggctgtgc caacatccgg
    14041 aaagtttgca aactcaacgc cgccaatcgt ttcattggca caaatatgaa cgtaggcggc
    14101 atccttagac aattgccaag tacttcgctc aggaatgctt ctgaagtgat cattaaggga
    14161 tgtggccgca acatgggctt ccccatattt ttgggcctcc ttcactgatt tttcagacca
    14221 aatccctgtc acaataaaat cagccttggg ctttttggca agcggcatca ggttcatggg
    14281 aatggcagca ttctgaccaa taccaccgcc ttgcaataac aaaatctcgt aatcgtcggg
    14341 gatggacatt agctcacgaa gatcggcaag cgtttcttca tataccgcca tgaactcttt
    14401 gctgcgatgg ctgatctcca tcacgctcgt accaagccca tgccaattga gcaactcatc
    14461 agcagtctgt tgcaagacct cctctggcaa ggttgctgga cccgctgcga agttataaat
    14521 gcggcggtca aagctcatga tagtttacgg tttaggatcg atgagcttag gcatcgcctg
    14581 tcggtggctc tgaatctttc tcagactgct catcaccaag ctcatcatca tcggagtcgc
    14641 tttcagcaat ccgctgcaga ccagataaat gcgttccttc atcgacattg attaaggtaa
    14701 ccccttgcgt ggatctgccc atctcccgaa tctcagagac gcgggttcta accagaacgc
    14761 caccggtggt aatcaacatg atctgatctt cgggtgagac taatgctgca gcaacaactt
    14821 tgccattacg ctcgctagtc tgaatcgcaa tcatcccctt cgtgccacga ccatgacgag
    14881 tgtattcagc aattggcgta cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca actagcatcg caatcacttc
    15001 ctgaccttcg gccaagttca tgccacgaac cccgcgtgct gttcgaccca tcggacggac
    15061 atcattctca tcaaagcgca ctgccttacc cgcgtcggag aacaacatca catcatgctt
    15121 gccatccgtg attgctgcac cgactaagaa atcgccttca tccaaatcaa cagcgatgat
    15181 gccggcttta cgaggattgg agaaatccga taaacgggtt ttcttaaccg tgccgcgtcg
    15241 ggttgacatg aaaacatatt gatcatcctg atagcctttg ataggcaata tgaccgtaat
    15301 cttctcgccc tcaatcaagg ggaacatatt aacaatcggc ttacctctag aagttcggct
    15361 gccttgcggc acctcccaaa cttttaacca atacatccga ccgcgatcgg agaagcaaag
    15421 aatggtgtcg tgcgtattcg caacaaacaa ggtatcgatc caatcctcat ctttggttgt
    15481 ggctgcttgc ttgccgcgcc caccgcgttt ttgagcacgg tactcgctta agggttggct
    15541 cttcatatag ccagcatgcg acagggtcac caccatatcg gttggggtaa tgaggtcttc
    15601 tgtgaaaagc tctgttgcgt tcatctcgat atatgaacga cgacctgtat caccacccgc
    15661 ctgaccaaac tcgtcctgca cttccttcaa ctccgcctcg atgaccttgg tcactcgatc
    15721 tggtttagcc aaaagatcta agagatcagc aatctgatcc atcacctctt tgtactcagc
    15781 aacaatcttg tcttgctcta accctgttaa gcgttgcaag cgcatttgca aaatctcttg
    15841 agcctgtcca tctgaaagac gatagagccc actgtcttgc ataccgtact ctggcaacaa
    15901 accctcgggt cggaatgcat tgcgtccacc aggtgcctcg ccctcagctc ttgcgagcat
    15961 ctcacgcacc aaagaagaat cccacgcttc tgccatgagc tcttgtttag cttcaactgg
    16021 attagcggca gctttgatga tggcaataaa gcgatcaata ttagctaagg caacagcaag
    16081 accttccaaa acatgtccac gctcacgcgc cttacgcagt tcaaagatcg tccgtctggt
    16141 aatcacttca cggcgatgtg ccaagaaata ttccagcatt tgctttaagt tgagtaagcg
    16201 tggttggtta tccaccaagg ccaccatatt catcccaaag ttatcttgga gttgcgtgct
    16261 cttgtataaa ttgttgagca cgacctcggg cacctcaccg cgcttgagtt caatgactac
    16321 gcgcattcct gatttatctg actcatcacg cagatcagag ataccctcaa tctttttatc
    16381 attgacaagc tcggcaatcc gctcgagtaa gtttttctta ttaacctgat aaggcaactc
    16441 atcgacaata attgcctggc gttggccttt gtccatatct tcaaaatggg tcttggcgcg
    16501 cataactact ctgccccgcc cagttcgata gccctctcgc actccctgaa cgccgtatat
    16561 gattccggca gttggaaaat cgggagctgg cacaatttca attaactcat caaccgtgca
    16621 ctcagggtta tggagcacgt gtagacaagc cctgataacc tcatctagat tatgaggcgg
    16681 gatattggtc gccataccca cggcaatacc agaggatcca ttaattaata aattaggtac
    16741 tttggcgggg agaatcaggg gttctttttc gctaccgtcg taattggggc caaaatcaac
    16801 ggtttcttta tccaaatcgg ctaatagctc atgagctatc ttgcgaagac gaatctcggt
    16861 ataacgcatt gctgccgcgt tgtccccatc aactgaccca aagttaccct gaccgtctac
    16921 cagcatgtag cgtagggaga aatcctgagc catccgtacg atcgtgtcat aaacggcagt
    16981 atccccatgg ggatggtatt taccgattac gtcacccaca atacgggccg atttcttaaa
    17041 agcccgattc caatcgttgt ttaattcata cattgcaaac aaaaccctac gatgaacggg
    17101 ttttaacccg tccctcacgt ctggtaaggc tcggccgacg ataacgctca tggcgtagtc
    17161 cagataggac cgccgcattt cgtcttctag ggatatcggg agagtttctt tagcggcttg
    17221 ttccatctcg aaataatatc atttcgccgg cgcagaacct gtgctaacat tctgatagtt
    17281 ggtacctatt ttaatattga cgcttaaagc ttttatgagc ttttaggcat ttagtctgca
    17341 ttaacttatt taaatgtttt ttgaggataa aaaatgaaca aaaccctaaa actgatgctc
    17401 gcagcattga ttactatttc tgctggttca gtcatggctc agccaaagaa tgttgacaac
    17461 tgggtcaact ctgccggaac tccttggaga aacggcgatg gcacattgtg ctggcgtgat
    17521 gctacctgga cccctgctac tgctgctgct aattgcgatg gctggcttgc tccaaaacct
    17581 gcagctgctg cgcctgctgc tgctagcaaa gtagctcaga agaaaattac cctccaagca
    17641 gacacattgt ttgactttga taagtcgacc ttgaaatcag agggaattgc tactctcaat
    17701 aaggttgctc aagatattac taagatgaag cttgaggtag tgattattgt tggttacacc
    17761 gacggtgttg gtaccgatgc ttataacatt gccctaggtc aacgccgtgc aaacgctgtg
    17821 aagtctttcc tcaccaatgc tggtggtgtt gaggcaactc gcgtttacac cgagagcaaa
    17881 ggtaaagctg atcctgtagc tagtaacgct actgcagaag gtcgtgctaa gaaccgccgt
    17941 gctgtgatcg aagtagttgg tacacagcca gttaagtaat ctgtctctta agacttcaaa
    18001 agcccggttc gccgggcttt tttattggtt cttttgtata gtggtggttt ccagcccata
    18061 agtgatttaa gccatgagta atgtagacca aagcgagatt gataaattta gcgccctagc
    18121 ccatcgctgg tgggatccta caagtgagtt caaacctctt catgctatca atcccctccg
    18181 cttaggctgg attgagtcga tcactgcact ggctggtaaa aaagtattag atgttggctg
    18241 tggcggaggt atcttagctg aatctctatc caaagccggt ggcatcgtta caggaataga
    18301 tttatcggca aaggctttaa aggtggctga actgcatcaa ttagaaagcg gcacatctgt
    18361 tcgttatcgc tcaattgcag cggaggattt agctaaagaa gagcctgagc aatatgatgt
    18421 agtgacctgc atggaaatgt tagagcacgt tccagatcca agctcagtcg ttcaagcatg
    18481 tgcctctctt tgtaagcccg gtggaaatat tttcttttct actctgaacc gcaaccctaa
    18541 gtcgtattta tttgccatta ttggagcgga gtacatcctg aagctattgc ccaagggaac
    18601 acatgagtac gataaattta tcaagccatc agagttagca aacttcacaa ggcaagcagg
    18661 tcttgagcta aatgagatta agggtatgac ctataaccca attacccaga tctatcgact
    18721 aggcagcgat acggatgtga actatatgat tgctacacgc aaaccatagc catgccatct
    18781 cttccttcat ttgagggggt cttctttgat ctggatggca ccttagccga cactgctcct
    18841 gatctggtgg ctgcagcaaa ccgcttatta attgctaaga acaagttacc gatgccctat
    18901 gagactctgc gacccatggc atccgcgggc gccagaggat tgatccaaaa aagttttggg
    18961 atcaataaag atcatccgga gtttattgct ttgcgagatc aatttttaag cttctacgag
    19021 gaagctctct tggttcatag caagctttac gagggcatgg aagaattact gcaaaggctc
    19081 gaagatgaaa agcttccatg gggtattgtg accaataaac aagaacgttt tacagttcca
    19141 ctcaccgagc aaatgggcct catgcagcga gcgcattgtg tcgtttctgg tgatacgacc
    19201 cccttctcca agccccatcc ggaacccatt ttgcatgccg ctaagctagc taaagttgac
    19261 cccaaaaagg cactctatgt aggtgacgat attagagata tcgtatcggg taaggcagct
    19321 gggatgcata ccgctgcagc cttgtatggc tattgtggct gtgacgaacc ccctcaccaa
    19381 tggggggctg actaccaaat tcagcatcct tgggaacttc agcaaatcat ctttgcaaaa
    19441 taaggtgtgc aatttaagtc caaacgactt aaaattgtga ccccaatgca aggaattccg
    19501 gggtcgacat ggtttcgacg tggattacaa agcatcaagg gcataccgag gacccgttat
    19561 ctcgtaaatc aatgggaatg caataactgc taacgacgaa cgttacgcac tagccgctta
    19621 attgcggctg cccctgaact gattctctct tgggtcaggt agcgaaagct acatcagggt
    19681 catatacaag agataaggtc atctcgagtc acgagggatg attcgaaaat ctagtgaatc
    19741 gctaatgcaa agcgtgtcga ttcgcgttgc actggttaaa tcaaataatt agactaagta
    19801 tgtagaactt gttgtagagt gtttgcggac gcgggttcga ttcccgccga ctccaccatt
//
LOCUS       DDPQ01000008           17594 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_577, whole
            genome shotgun sequence.
ACCESSION   DDPQ01000008 DDPQ01000000
VERSION     DDPQ01000008.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 17594)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17594)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17594
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_577"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    302..340
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    854..929
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1211..1223
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2623..2737
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4121..4157
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5634..5700
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9394..9483
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9779..9888
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12002..12045
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4839 a         4273 c         3633 g         4251 t
ORIGIN      
        1 ctttttgcaa tctaggcaat atctatgcct tgggtttagg catggagcat ggagtgccgc
       61 gcgatatttt caagggactt gaattgcttg ctaagggagc ggagtttggc agccgtcaat
      121 cagcattcac gcttggctct ctttatggaa aaggggagtt cattcctcaa gacaagtcta
      181 aagcagtgta ttttttatct ttggcttcta agatgggtca cgaacaagca aaacgggtgt
      241 taattatttt tgcacacgcc catcctggcg agaatttcga tgctgagcta gatgaagcaa
      301 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctagccagaa gtaatttttt
      361 cccagttttt cttgaagact tgattgctgt cttgaggtcc agagtccaag tctttcgatc
      421 ttgaaatcat caatccattt aagcgagcgc ctggctcaat tccaatttgg ccgtaggtca
      481 catcaccatg cacaattgcg ccattgtgca actcgacgcg ctcggtacag tagacattgc
      541 cttctacccg tccggcaata atgacacggt aggcataaat atcaccctga actatgccat
      601 tttttcctac cgcgatactg acaacgccat cttgggaggt ctcgacatta ccaattactt
      661 tgccatcgat acgcagactc tcagaagcga ctaatcttcc atgaatctca gttgcttcac
      721 cgatcatcgt ctgaaaagac tcaacggttc tgcctaaggt tttgatcgat tgaggtttat
      781 tgccaaacat aatagattac ttcttaatga aatgaccctt gatcttgcca ccgcgctgaa
      841 tgtgtacgga ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tggaatcaac taatacggat ttagcggtta
      961 ccttaccctt gatgactcca gagatattga gcacaccttc agaggtaatg gttccaacaa
     1021 actcaaaacc ctcgctaata atcgaaggct tagtagggtt gatgcgattt gaagaccctt
     1081 cgtaaacact ttgctcagac tctgcggctg gctcaggagt gtattcagga tactgatttt
     1141 gtgaattctc agaagaatta tcttctgaat cgtttggttt cttcgagaac ataaattacc
     1201 taaatataaa nnnnnnnnnn nnncccacct tgccgcttga gcgaataatt aaagatcgtg
     1261 atgcagttag ttgttcggcc acttctccgc ggatatcgac tgactcggca gtgactttgc
     1321 cacggataag acctgttgac tcaacaataa cctgcttggc tgacaaatct ccttcaacaa
     1381 cccctgaaac ggatgcaatg tcgggcactg caaaatttcc cttaagaaca actccctcgc
     1441 ccacaaaaag acatcctgat tgattcgcct ctaccatgca aaacccttaa tttttgttca
     1501 ctaattggtt ataaacactg ctatttgtgg caattccttc gcagaactgc cacaaaatat
     1561 aaatttatca caattgcgtg tcaaagtatc aatcgaggcc ccaaaatggt gatttcaggg
     1621 aaaaccctcg tcttcattct tggcttaatg cttcgtttgg ccgccaccag agatatccct
     1681 gacaggcacc ttaatttcga cctctcctgc cttttggaac ttcagcttga ctggtaccga
     1741 ctcgccagcc tttaatggcg ccttgatatc cataaacatg agatgcaagc cgcctggttt
     1801 tagctctacc gatccgttgg caggcacatc aattgtcttg acctcacgca ttttcatgac
     1861 attgccatcc attgccatgg tgtgcaattg catctcgttg gcaacgggcg agctagccga
     1921 gattaattga tcggcgacgc ccttattttc aatcttcata aagccaccag caaccttttg
     1981 accaggagct gtggatcgtg tgtaaggatt ctcaatcttg agttgaccaa ctcggtactc
     2041 ttgatctttc ttatcattgt gcgcaatggc gattgctgag aatgccatca ggccgacaac
     2101 tcctaaactt tttaaataat tcatttgcat ttttgtttcc tttcattatt cataaaaaaa
     2161 ttaccaactc atggtctttg tgccatcctt aaataaggca tcacccgtta ccttcgggga
     2221 gcccatcttc accccaggta ataaatcgga ttccgtcaaa ccataatgtc gcaagcacat
     2281 gggacacata attacctgtc cgcccgcctt cataatgtca ttcaataagg tttgttgctc
     2341 tgcatacttc ccggcgccag acttcactcc aataaaaacg cccttatcgc ttaagaaaac
     2401 actcaatggg tggccattat caaaatgatg tttcccaaaa ctaagggcca tccgcgcgcg
     2461 atgtggctca tcggtagtaa gactgacaaa taacgggtcg ttgggtccag cgctgactga
     2521 aaaactaaca gctacagcga tggtcagtat aaaaatacgc aatgcgttca tattcattcc
     2581 taatcatgat tagatggaga tacaaaaacc gattactcga tgnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca acacagtaag gacaatcgag
     2761 catcacttca gatttgccat cagtttggag atcaatcgcc agtttgctgc cgtcagcaat
     2821 acagatctcc atggtgagcc cttgtgcatc tgaggccact gcaatagcct gggaaataga
     2881 tggggctaaa gaactcatcg caacagtgag tgccgtaatg cagtggataa gcttgcgaga
     2941 gaacatgcac taattttatc tgtatttgtg cagataaaga aaaaggggct tgcgcccctt
     3001 aatctctcaa tttgccagtg gtttaagcct tcttagcctt aagctttgaa aacaagggcc
     3061 atagcaacag aataagtgcc aatgccgtga tgctgcccac gaggtagttc gagaagaaaa
     3121 tcccaaggct gccttgcgat aacaacatag cttgacggaa agaatcttct gcacgatcac
     3181 caagcaccaa cgccaagacc atcggggcca ttgggtaatc aagtttctta aagaggtagc
     3241 ccaaaatccc aaagcccaac atgagccaga catcaaacat cgcattgtga acagtaaatg
     3301 ctcctaccgc acacaccaca ataattaccg gtgcaatgat cgagaatgga attcgcagaa
     3361 tcgatgcgaa gatcggtacg caggtcaaca ccacaaagag accggcaata ttaccgagat
     3421 acatacttgc aattagcccc caaacaaaat ctggcttctc aacgaataag agtggacctg
     3481 gctgtagacc ccagattaag agtccgccca acaatactgc agcggttgga gaccctggga
     3541 tacccaaaga gagcatcggc aacaaagcag ctgtacccgc agcgtgcgcc gcggtctcgg
     3601 gagcaacgat accttccatc tctcccttac caaacttgtc tttgttcttg gacatttgct
     3661 tcgcaacgcc gtagctcata aatgaagcag gagttgcgcc gccaggggta atacccatcc
     3721 agcaaccaat caaacagctg cgcaatgaag tagcccagta cttgggtaac tttgcccagg
     3781 tctcaaacac cacttttgcg cggatcttag cgctcttgcc agagaacttt aagccctctt
     3841 ccatcgattg caagatctcg ccgataccaa aaaggccaat cacagcgatt aagaagtcaa
     3901 agccacgcat gagttcggta gacccaaagg tcaaacgtaa ttgaccggtc acggtgtcca
     3961 taccaacggc agctaaggca aagcccaaca tcatggccga gatcacctta aagggtgaac
     4021 ctttgttcat gcctacaaag ctacaaaagg ttagtagata gactgcaaag aactccggcg
     4081 gaccaaactg cagggcgaac ttagcaacca aaggtgccaa nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnntca ccggctttac catcttgggc cattgggtaa ccatcaaagg
     4201 tcgtagcgac tgaccaaggt tcgccaggaa tattaaagag gatcgaagta atcgccccac
     4261 caaacagcgc accccaatag atacaagaga gcatgatgat tgcggaggta ggaggcattg
     4321 tgaaggtgag aggtaagagg atggcaatac catttgcacc acctaaacct gggagaactc
     4381 caataatcac gccgagggtg acaccaacca gcatcaacat gatgttaaag ggcgacatcg
     4441 caacagcgaa gccaccaaat agggcgttaa tttcttccaa cgtgagctcc tttttttcta
     4501 atctttatcg ttatcggtgg gcagacttat tgcaagccaa taaatgccaa tgggttaatg
     4561 agtgacccgt ggggcaatgg gatttggaac cagtactcaa acatcatgaa tagcgcaaca
     4621 ctgacaccca gtcctaccgc aactgccttc caaacaacat acttgcccag ccaaaccata
     4681 aagattgcga tataaacagc tgaggcaaca taaataccga tgagctgaac gcctaatacg
     4741 tacactgcgg caggaaccaa gaccgccatc acttgacgaa atggttcttt ttctacaaac
     4801 gcttcttctg cctgttcttt gtttttgaat gcctgaaaca gagtaattga actcgaaact
     4861 agaatgatca aactaatata gaacgggaag tagccagact cgggaccatc actaccccac
     4921 tttgctccta acttaatact gccggtcatt accactagac caactacgag gaacaaaaga
     4981 gcaacgataa tctccatcgc tctaacgctg actactgagt tttgatttga gttctctgac
     5041 ataacttact cttcttctaa ataaacaatt agctaaaaaa ataagacccc gttatgaggt
     5101 cttatttcta ttacaacaaa ttacttcgca aggaaaccag cttccttcat caactgcgca
     5161 tgaagagcgg cattcttttc taaccaagct acatactctt taccggtcat aaagctttgg
     5221 ttaaatgcgc catcggccat aaacttcttc caatcagcgg tctcgcgaac tttcttgaac
     5281 aggtcgatat agaaatcaac ctgagcctga gtcacgcctg gtggcataaa aataccgcgc
     5341 aacattaggt aatcggtatt gacgccaact tccttacagg ttgggacatc attccaagat
     5401 tgggttggag taattttttc cttgtaaggc atacgttgat catcaaatac gcactgcgca
     5461 cgcaacttac cggcacgcca ttgagcgacc gcctcgattg gattgtttac ggtcgagtca
     5521 acgtgattac caacgagctg taccgcaacg tcaccacctc ctttaaatgg aatgtaggtg
     5581 aacttggttc cggtagcctt ctcgagcgcc acagtaataa tttgatcctc ttgnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 tatggtttct cagcattggt ccacaacaca aactgatcta aggccaacat ggctactggg
     5761 gtcatttctt tccagctaaa tggcacgcca gttgctaatg gggtagtaaa caagttagat
     5821 aaagtaataa cgatcttgtg tgggtcgccc ttggcttctt taatcgccaa gaaaccttca
     5881 gcacccgcac ccgcaccctt gtttacaggg atcatcgact gcttcattaa attgttctta
     5941 gtaacaatgc cctgaatcat gcgagccatc tgatcggcgc cgccgccagg tcctgcagga
     6001 atcacaaact caactgcttt cgttggttcc catttcgcct gagcgtgtgc tgttgacgtt
     6061 gcagcaaacc cagccgcaag cgcaacactt aataaagtgg atttcaattt aaaacgcatg
     6121 gatgctcctc caaataaaat gacaaagttt tttaatttgt tatgtgtctg ttttgtaaac
     6181 tcattcttca acaaaatagc gtgctagaaa ttgacagaca tcaacaaacg aaaattttca
     6241 cataagtagt acacaccatt actagtgaaa acccgtattt caggtaggaa tcatgaaata
     6301 aatcctggtg ccggaaatag gaatcgaacc tacgaccttc gcattacgaa tgcgctgctc
     6361 taccaactga gctattccgg caataaccct atgtaagaca gaggattgga tggagtgacc
     6421 acttctattt tagcccagca aaatcaaggg ctttctcttc atacctgcta atcacaagcg
     6481 cgctgcaatg gcctttgtaa tgacttcgcc aacatcgggc gataaatgac cagcctttgc
     6541 aacgtgatgc aaggcagcaa gcatgtgctt tgaatagggc tttgcaaatt ttggccagcg
     6601 gtctaaggct cgagccaaac gtgctgccac ttgaggatta atcttgtcca atgcaatgac
     6661 acactcgatc cagaatcgat aagcacttcc atccgcctga tgaaatcctg ctgggttatt
     6721 gaggcaaaac gcatggatta agctacgcac acgattaggg ttatttaact taaaggcact
     6781 gtgttgcatt aaggccttaa catccatcag aatcgatgtt cctatttgcg ctggtcgtgt
     6841 cgcttgtaag gcgaaccatt tatcgattac tagggcatct tctgcaaagc gctgatagaa
     6901 atcagctaac gcatttttag catgtggggc atttacagca accaatgcct gtaaagccgc
     6961 aaaccgatca ctcatattat tggcgagctc atactgttct tcggccctct cctgccaatg
     7021 aacatctccg ctttccaaga gcatcatcaa agcaaggttt ttgagtgcgc gcttgcccat
     7081 acttttagca tcggcccgat atgcccccgg gctttgatga tcagcgtagg tcaatgccca
     7141 ctcatcctgg agtgcttgcg ctaatgctgt tttaaaacga cgcctagccc aatgaatctc
     7201 ttggggctca accactttgc actgctcata taaataggtc tcagcgggta gagtaaaaat
     7261 aagctcttta aaggcggggt ctaaatgagg atcttgcaag atcactcggt aagcatcgat
     7321 taatttggta ttgggtaagc gcccagccaa gatcatttgt tgggctagtt tttgagccga
     7381 ctcccagcga ctaaagggat cggtgtcttc ggccaataga gttaaaagct catcctcact
     7441 ttgcttgtgc tccaaaatga tgggggccga gaaatctcga ttgatcgata aaactggttt
     7501 agcagaaatg ttctgaacta accagctttg ctccgcttgc gtgagctcta ataagacctc
     7561 ttgtgcaaaa taggctcgct ctttttgaga gactttgctg cccttatcta gtagaagact
     7621 tgcttttaat ggaatatgaa aaggcgcctt cttgttctgc cctggcgtct ttggacaatg
     7681 ctgtctgaga gtcaagcgat actctttttt cttagtatcg taattttcta acacggtcac
     7741 tcgcggagtc cctgcctggc tataccagtt cttaaactgt gtcaggtctc gttgattggc
     7801 atcggccatc gctgcaacaa actcatcgca ggtcactgcc tgaccatcgt gtcgcttgaa
     7861 gtaaagatcc atccctttgc gaaagccatc gcgccccaat agggtttgat acatgcgcac
     7921 aacctcggca cccttctcat aaacggtaac cgtatagaag ttattaatct cttgatactc
     7981 atctggacga atgggatgcg ccatcgggcc cgcatcttca ggaaattgca cttgacgcag
     8041 taaacgaacg tcctcaattc gcttgactgc ccgcccagaa tcagtaccca tttgatcggc
     8101 tgagaactct tgatctctaa aaactgttaa gccttccttg agcgataact gaaaccaatc
     8161 tcgacaggtc acgcggttac cagtccagtt atggaaatac tcatgcgcaa caacactctc
     8221 aatattggcg taatccccat cggttgccgt ctgcgcatct gccaagacaa atttggtatt
     8281 aaaaatattg aggcccttgt tctccatagc gcccatattg aaatcaccca cggccacgat
     8341 catgaagcgc tccagatcca gctcaagacc aaaacgcttc tcatcccacg cgatcgaact
     8401 ctttaaagaa tccattgcat gttgggtctt ctgaagatca cgcggctcaa cccaaatttg
     8461 caggagtttt tgggcaccgc tttgagattg aatggattct tctaaacaca ctaatttgcc
     8521 tgcaacaagg gcaaataggt aggatggctt tggaaaggga tcctcccaga ccgcttgatg
     8581 ccagccatcg cccagatctt cactcgcaat taaattgcca ttggataaga gaacaggatg
     8641 ctcttccttg cgagcgcgca gggtcactcg gtagcgcgcc atcacatcgg ggcggtctaa
     8701 aaaataggta atcttccgaa aaccctctgc ttcgcactgt gtaaagaaat tgcctcgtga
     8761 tacatagagt cccatcagtg aggtattttt atctggcaca caggaagttt taatttctat
     8821 ctgaaatttc tgcttaccct tgttgggcaa gccatggatc accatgcgct caggcgtaat
     8881 ttctacatgt cggtgtgcct gtccgttaac ccgcaagcta atgaactcga gatcttgccc
     8941 atcgaggatt aacggaactt ctgggtgact ggccgccgta cggggactcg gcacgacctc
     9001 aatcacgctc tggactatnn nnngtgcgat ggggctgtaa ctcaaaatct agctccactt
     9061 ggccaaattg ataatcgggc gccttgtatt gcagacgcaa aaagcctttg ctagtttcag
     9121 ttctcattac agaattctaa tttgttatgg ctgttgggac tgatggctct acgatgacca
     9181 taccaaggta atgagtccga gggcaatgaa aatgatggct gctacccaat gcacaagctt
     9241 aatcggtaag cgtttggtaa attgctttcc taaccaaacg gccggtgcat tcgctaacat
     9301 catccccaag gttgtcccga ctgtgaccaa gagcacgctc tcatacttag cgccaagagc
     9361 aatcgttgca atctgtgttt tgtcgcccat ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnncctgctt tgcattttca tcttcctcca atttgtctgg gatcaataac cacagaccaa
     9541 tcgccaagaa tccaactccc aagatccaac gcaagctgtc tgggctcaaa aagcccgcga
     9601 cccaatgccc cacgtaagca gcgcaggcgt gattaaccat tgttgcaagg aagatgcccc
     9661 acacaattgg ccaagcgtgc ttgggatagc gcgccgctag catgagggat aaaagctgag
     9721 ttttatcccc aatttcagca agagcaacca ccccagtaga aataaatagt ccagaaaann
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc atcctaattt
     9901 ttctgttgaa agtaaaccca tgactttacg tgaaggtaac ctggaagccc caactcggca
     9961 ccccttggat tggaccaatc ccgactttta taacaccgag aaactggaag cggagatgga
    10021 gcgggtattt gatctctgcc acggctgccg tcgttgcgtg aacttatgtg gctccttccc
    10081 agcccttttt gatttggttg atgctacgga agatggtgaa gttgccggcg ttgccaaggc
    10141 tgactatcaa caggtggttg atcaatgtta tctctgtgat gtgtgttaca tgaccaagtg
    10201 tccttacaca ccgccacatc cttggaatat tgatttcccc catttgatgc tacgcgctaa
    10261 ggcggttcat ttcaaagaag gtaaatcgag tctgcgcgat aaatttttat cctctacgga
    10321 cgcacttgga cacttcgctg gtattcctat cgtgactcaa gcagttaatg cattgaacag
    10381 cactggggtt gcgcgcttag cgatggaagg tgctttgggt gttgataaaa aagcctggat
    10441 tccagaatac gcttccaaga cctttccaca acttgcaaag gtatctgccg ctcacgctgt
    10501 gaaagacggt caacgcactc ccggtaaagt ggcgatctat gcaacctgct acatcaacta
    10561 caacgaacct gggattgggc aagacctcat caagatcctc aatcacaatc agattccgta
    10621 tgcattggtt gataaagaag cgtgttgcgg tatgcccaaa ttagagttgg gggatctcga
    10681 atctgtaaaa gcaaataaag aaatcaatat tcctaagctt gctaagttag cacgtgaggg
    10741 ctatgcaatc gtgaccccaa ttccttcgtg taccttgatg tttaaacaag aattgccgct
    10801 catgtttcct gaggatgctg atgtacaagc agtcaaggag gcgatgtggg atccctttga
    10861 gtacttcatt gcacgcagca aagatggtct cttgaaccaa gactttaagg aagaattggg
    10921 tcatgttagt taccacgttg cgtgccactc acgcgtacaa aatgttggcc agaaaactgc
    10981 tgaggctctc aagctcattc ctggtactga agtgaatgtc gtagagcgtt gctctggaca
    11041 ctcgggcacc tggggcgtca agaaagaatt ccatacgatg gcgatgaaaa ttggtcggcc
    11101 agtatttaga agcatggcag aagagtctcc caattacatt agttcagact gccaactcgc
    11161 tggccatcat attgctcaag gcatggatga gctaggacta ccaaaagtag cgatggcaca
    11221 tcctttaacc ctaatggcaa aagcctacgg actttaatcg cttcaatttg aaaacaggat
    11281 gaatacagga tcaataggta tgggaaaaat tacgatcgac agcttactaa gtcttgaggc
    11341 ttatcacaag gctcgccctg aaatgcggca acgggcaatt gcagagcgca aattacgtac
    11401 cgtgcactta ggcgagcacc tcactctcat ttttgagaat gagttcttga tgcgttatca
    11461 aatccaagaa atgctgcgag tcgagaaaac ctttgaagaa gaaggtattc aagatgaact
    11521 tgacgcctat aaccccatgg taccgggtgg ctctgatttc aaagtcacga tgatgattga
    11581 ataccccaac gaggcagacc gcaagattgc tctgagcaaa ctagttgggg ttgaggatcg
    11641 catcttcatt gaggtcgaag gtcagccacg ggtgtatgcg ctagcggatg aagatttaga
    11701 aagaactacg tctgagaaaa cttcagcagt tcacttctta cggatacccg tttcagtcgc
    11761 catgaaagag gctttgaaag caggtgcgca gatgatggtg ggttgcgacc acaagggcta
    11821 ccccatgcat gtcgaaaccc tccctcatga aacactggcc tctttagtta cagatctcag
    11881 ctgaatacaa actgctgatc gcctcatcgg cagcgatgta atttaacttc tgtgccttcg
    11941 caaccggggc agaagttaga tgccccttaa agacactcaa gccattttta agatgtggat
    12001 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgcg accgcaccgg
    12061 gcatattggc cacacaataa tgaatcactc cgtcgaccat atatgtcggc tccgcatggg
    12121 ttgtcggtaa tgatgtttca aagcaacccc cttgatcaat cgccacatcg acaaccactg
    12181 aaccgggtcg catcgtctgg atcatggcct tactaagtaa cttcggcgca gatccgccgg
    12241 gtaataaaac agcgccaatc accacatcgg tatctttcac tgcagcttca atactcatcg
    12301 cgttcgagaa caccgtacgc actcgattgc cataaagcgc atcaatttgg cgtaagcgct
    12361 caagatcgcg atccatgatg ctgacctgcg ctcccatacc taaggcaatt tgcaatgcat
    12421 ttcgtccaac cacgcctgcc cccaagatca acacctttgc ggggctcacc cccggaattc
    12481 cacccatcaa tatcccagga ccccctctgg atcgctctaa atgggtagcc gccgcctgaa
    12541 tcgacatcct acctgcgacc tcgctcatag gagccaataa aggtaaagtg ccatgagcag
    12601 aggtcacggt ctcatacgct atacaaattg ctccggagtc catgagagcc ttagtctgct
    12661 ctggatcggg tgccaagtgt aaataggtaa acaagatttg gtcttcgcgc agtaatgcgc
    12721 actcatgtat ttgtggctcc ttgactttca caatcatgtc agcgcgtgca aagatatctt
    12781 ttgcgctatc ggataattct gcgcccgcct cttgatagga cagattccca aatccaatct
    12841 gagcgcctgc tccactttgt acgatcaccg tatgaccttg tttacaaaga ccattgacaa
    12901 gactgggggt caagccaacc cgaaactcat tatttttaat ttcttttggt ataccaataa
    12961 tcatttttgt tctcctaatt gaaatttctg ttttttatcc aagccaaaaa cgtaatacaa
    13021 gaccatatac aaagctaata agattggtcc aacgattaag gcgatgcgtg catcttcatg
    13081 aaaactcatc aacacaatca ccatgaatat gaaccctagt gcaaaccaac tggaatacgg
    13141 ccaccatggg gcgcgatacg ttaactctga tagctgcttt gttgataaag aagatcgaaa
    13201 gcggtagtga gtaaataaaa tggcaatcca caccatcagg ccaacaaagg tcacggctgc
    13261 catcatgtaa tgaaaggctt tgtccggaac aaaatagttg agaatcacgc caatcatggg
    13321 cacgcacact gtcccaatca ctgcgcgatg cggtacacca ttgttggaca aataggcaaa
    13381 gcgtcttggt gcatagccat tagcagataa agcccataac aaccgaccgc cactaaaaat
    13441 accggcattg caagaagaga gtgcggcagt aatcacgaca aaattaataa cgccagccgc
    13501 ttctcgtaaa ccaattcgct caaacatcgc aacaaaaggg ctgccctgct gacccacttc
    13561 gttccacggg aagattgcca gaatgactag taatgcgcct agataaaaaa tgaggatgcg
    13621 ccacgttaat gagtcaatcg ccatggggat tgtttgctta gggttttgtg cctcgcccgc
    13681 agaaaggcca atcatctcaa tacctacata ggcaaataag accatatgca atgacagcaa
    13741 caagccacta aagccattgg gaaagaagcc gccgtgttgc cacaaattac tgaggcccat
    13801 tggtacccaa tcattggtaa atcccagaaa aatgactgca ccacctaaga caatcatcaa
    13861 tacaatcgca acaattttga tgagcgcaaa ccaaaactca aactcaccaa aaaccttcac
    13921 agcaatcata ttgattaagc ccatcaagat gatggaagac agggcccaaa tccattgcgg
    13981 gatatctggg aaccacattc ccatgtaaat accaactgcg gtaacttctg caatagccac
    14041 aacaacccaa taggtccaat agccccatcc caccatatac ccagtgagtg ggctcacata
    14101 cgtgctggca taactagcaa atgaacctgc aaccggttca tgcactgcca tctcacccaa
    14161 ggtgcgcagc accacaaagg caaccattcc agccaataag taagctaaca aaattgaggg
    14221 gcctgcaatt tggattgccg tagcggatcc taaaaataat ccgacaccaa tcgtagaacc
    14281 taaagccatt aaacgaatgt gtctagcctt gaggtgccgc tttaatccat gctctttatt
    14341 ctccaatgag aactcctttc attcttgcga tggcaattcg ccaacgaatg aaagtctagg
    14401 gagacaaaag agattgaacc agcatctaaa acctataaaa gtattaggag ttagatttat
    14461 acaaagttgt ttttatcaaa taactttgat atcaagaact aatgaaacaa gagaaataga
    14521 tggactacag aaaaatcaga aaattcttac agggatagcc ctagtatgtt ttgaaaatta
    14581 tggacgaaaa aaaaccccag caagctgggg tttcttcaag caagtgagag aattacttct
    14641 tctcagctgg cttagcagca ggagcagcag cagctggctt agcagcatct ttctttggct
    14701 cttctttctt agctggagca gcttgagcga aaacaccagc agagaacaaa ccagcaacta
    14761 acatagcgac gatctttttc atgtaaaact ccaaaattaa attaataaac gtagtctcct
    14821 acggatttct ttacaaccac cttaattcaa accagtgtag ctacaaatgg ctcttattaa
    14881 agcttattcc ctcaacgcct ctcgaaagag aaccgttgac agatttatta cttgaaacat
    14941 atttccaagt aaaacaggag tttagcaaaa tcttgcttaa gagccattgg ctataaggnn
    15001 agttcagcag ccgcaacaaa gccaaatgtt gccgtcacac tcaccgcaga gccatagcca
    15061 gagcaggcca agcccccatt gcccgcgccc agccttggct catgggagta aaccgcccga
    15121 atacccatcg gctgctttaa gtccctacta aatgaatggt ttttacgtaa gtctgccctt
    15181 atttttgcca gaaatgggtc ttgaaccgtt ttagctaaat cggctgcgct gacgaagctg
    15241 gggttgacct tgcctcccgc gccaccgcac atcaccaagc caatttgcaa tgatctgcaa
    15301 tagtgggcta aggcgacctt tgcaggaaca tcatcgcagg catctactac ataagagccc
    15361 tctggtatca actccgccaa attgccctca gccagaaaaa catcatgtgt cgttagccga
    15421 atagctggat taattttgcg gatccgctca gccatggcat ctacttttgc cttgccaaac
    15481 tctccttcca tggcatgcaa ctgtcgattg atattacttg gcgcaatgtg atcaagatca
    15541 atcaacacca agtgcccaat cgctgagcga gctaaggctt ctgccgccca ggaaccaacc
    15601 cccccaagac ctatcaccac tgcagtagct tgcgtaaact ttcggtaagc gtcttcacca
    15661 taaagcctgg ccacccccgc aaaccgacgc tcgggcatct ctgcctcctt gttctgattt
    15721 actctttcgt tcaccataag ctctctttat actcaatcca tgaatgaaac ttctcaatcc
    15781 ctagccgctt tgcgcaaaaa ctatacccta gggcagttat ctgaagagga tgtgcctaaa
    15841 gatccaattg ccttatttga gacctggttt gcccaagcaa ctcgtgcgga gtgccctgaa
    15901 cccaatgcca tggttctagc aactgccaat gcaaccggca cgccatccgc tcgggtagtc
    15961 cttttaaaag gcttaagcca aggagatttt tgtttcttca ctaactatga aagtcaaaag
    16021 ggtaaggaat tggctgagcg cccccaggct tccttgctct ttcattggca tgaactagag
    16081 cgtcaggtgc ggatcaatgg ggatgttaca aaactaagct ctaccgagag cgacgaatac
    16141 tatctccaac gtccgcctgc gtctcgtatt ggggcttggg catcacccca aagcaaagtg
    16201 attgagagtc gtgccttttt agaacaggaa gaattacgct ttcagaaaca atttggtgac
    16261 aacccaaaac gcccacctca ttggggtggc tatcgtctca agcccagcag tatcgagttt
    16321 tggcaaggcc gtccatcgcg tttacacgat cgcattctct atgcccgcga tggttcgggt
    16381 tggcaaattg ctcgattagc gccttaaaca aatcctaagc gtttttatcg aattagcatt
    16441 tggaatgttt tcttaaattt actgacctta ggtcctacta ctgccatgca gtagccttgg
    16501 aaggggtggg tctgaaaata gttctggtga tagtcttctg ctggataaaa ggtcggggca
    16561 gcctttatct cggtcacaag cggacggcta aagtgtttgc cctggcttag ttcagaaact
    16621 agctctttcg ccacatgcat ttgctcatca tcaagggtga agataacgga gcgatagcga
    16681 gcaccaacat cgttcccctg atagttcagg gtcgttggat catgaatcac aaaaaagatt
    16741 tctaggatct gccggtaact tacgatgcgg ggatcaaact gcaactgcac tacttcagcg
    16801 tggccggtag tctctgcgca aacagtttcg tagtcaggat tgggcacatg tcctcccgca
    16861 tatcctgaaa caaccctctc aacacccttg acctgctgat agacagcctc aagacaccaa
    16921 aaacagcctc cacctaatat agcctgctga actccatctg acataacgca attccttatc
    16981 tagaaaacaa cttgatcact caatactaag cgtactgcaa aagcacagaa aagtagacca
    17041 acaacaaacc acaatgttgc ggtccatcga gggtgacgcc taaagatccc caccaaacct
    17101 tgtccagcca aaatcaggct agttaaatac gtaatgctaa taacttgcaa gatgatcgct
    17161 aagaacagaa aactgatcgc agggttagga aattctgggt ctacaaattg ggtgaagaaa
    17221 gaaatgaaga agaagatagc cttcggattg gttagagata aacccaaagc cgcaataaaa
    17281 gggtgcatct gcaataaacg accacccatg gatgtggcct taatttgctc tcttccccaa
    17341 tagcgttgcc atgcaatttg gattaaaccc caaccaatcc atagcaagta aacggcgccc
    17401 actaagcgca gaaccataaa taaggtcggc gaactgtgta acagcgatgc agcgccaaat
    17461 accacggcca agatcaacac cgaatcgccc gccacgatac ccaaagacgc ccaaactcca
    17521 gggcgccagc cggactggga ggctgtggtc aaaacatata aggagttagg tccaggaagc
    17581 aaaacaatgg cgat
//
LOCUS       DDPQ01000009           18789 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_3026,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000009 DDPQ01000000
VERSION     DDPQ01000009.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 18789)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18789)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18789
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_3026"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    5705..5830
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6846..6868
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7131..7166
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9041..9105
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4878 a         4470 c         4074 g         5117 t
ORIGIN      
        1 taaagactcg gtatttgtgc ccttctttgg cgttgagacc aacaccatta cagcaatctc
       61 gcgcttggcc agcatgactg gtgcagaggt ctgcatcatg aaaacgaccc tgaagccaga
      121 tcgatctggc tatatttgcc atattagtaa accccttact aatttcccga ccaaagatcc
      181 cattgcagat actgcgagac tcaatcgttg ttttgaagat gagatccgct tacgtcctgc
      241 cgaatactat tgggttcaca aacgcttcaa gaatcgacct gctggggagg ctaagattta
      301 ttagtgtcaa aatagagtct tcctggagac cctactatga aaaatcacat taacgccaat
      361 caaatcaaga tggcttatca aatcgatggt ccggaaaatg ggccgacagt catcatgagc
      421 aatagcctca tgtccagcct agaaatgtgg gatagtaata tcagcgctct aaccgatcgc
      481 tatcgcgtga ttcggtatga cacccgcggt catggtcaaa ctcaggtgac tgaaggtcca
      541 tatagcattg ccctacttgc gcaagatcta gttgccctca tggatgctct gcagattaaa
      601 caagcgcatt tggttggact ctcgatgggc gggatgatct gccaatacat tggtgctaac
      661 tatcccgatc gcgctctttc cctaagcctt tgcgacactg cgagcgagat gcccccacgt
      721 agcttatggg aggagcgttt tgctatcgcc cgcaccagtg gcattatggg tctcgtcgat
      781 ggaactataa agcgctggtt tctgggtgat tttattcaac gcgagcctca acagattgaa
      841 cacgttaagc acatgatttt gggaacccca gtggatggtt acctagcctg cgccagcgca
      901 gtgcgtgata tggctcagtc cactatgctg ctcaagatca aagcgcccac catggtgctg
      961 gtcggcaaac aagatcctgc atgtaccgtt gaccaagcaa ctgtattaca tcgcctcata
     1021 ccgcactcct cgatggtagt ccttgatcaa gctgcccatc tcgccaatat cgaacagcct
     1081 caagccttta atactgcctt gcgaggcttc ttagactctg tggcatagtt agccctagta
     1141 agttaatcat aaaatcaaga ggagacaaca tgcatattcc tttcaatgcg tatcgaatca
     1201 ctgcgctcgc attatttact gggatgctct cgttgcagcc ccttttcgta agcgctcaag
     1261 ctaactatcc aaataaaacg gtcaagatga ttgtgcccct gactccagga tcaggcgctg
     1321 atattgccgg acgtattgtg gctaagaact tatcagagag ctggaagcag cctgtgatta
     1381 ttgagaatcg ccctggtgct ggtggcttgg tgggcactgg tgtcgtcgtg aactctgatc
     1441 cagacggcta tacgctctta gtgcaatcgg cttcttatgc agcgaatcca gccatctata
     1501 agaaattacc ttatgacctc aagagcctaa aagatgtcaa catcttgggt actagccctt
     1561 atgcattggt tgtatccgca gagagtccct acaaatcctt aaaggacttg gtcaacgccg
     1621 ccaagagtaa accagagatt attccgtttg ccagcgcagg cgttggtagc tcaacccacc
     1681 ttgcggctga gtactttaat caaaccatgg gcatcaagat gttgcatgtt ccttttaagg
     1741 gatcccctga agccattcaa gaaaccattg cgggacgcac tgcctattac atggcgccac
     1801 tacaaaccgc gattgctcaa atacaaggcg ggaaagtgcg tgcacttggt ttaacgagtg
     1861 ctactcgtgc tgaggctgcg cccgatattc cgacgattgc tgagcagggt ttcccgaact
     1921 tcgaaattgg tttatgggtc ggtgtatggg cgccatcagc aacaccggct agcgttctcc
     1981 aaaagatcaa taccgatatc aaccgtgctc ttcaggattc tgaggtcaag gctgcttact
     2041 ccaaagctgg gatcactatc aagccaatga acttgtctga gaccgaaaag ttcgttcgta
     2101 gcgagatcac taagtacact aagattgcta aagacgctgg aatcgaacca caataacccc
     2161 ccgctttgcc aagcaccaga ccaggtcctt aaaaaacctg gtctcgttct ttgtgaacat
     2221 gctactgatt gccacgccca tatttgtggg ccagcatccc tttatcctta ctccccaaaa
     2281 cggatttaca ctccgcccga tgctctcctt cctcaatatc aaacactcct tcagtcaatc
     2341 ggcatagagc gtgctgtatt agttcaaccg agtatttatg ccgatgacaa tcgtgccctt
     2401 ttggccgcac tcaaaacaga tctactcaat ctgcgcggtg ttgctgttgt ggattgggat
     2461 atctccgaac gggacctaga gaaaatgcac cacgctggag ttcgaggagt gcgttgcaat
     2521 attgttgatt tagcagatgc aaagggagtc ctcccgattc tgaaacttcg ttatctggca
     2581 gataagattg ctccgtttgg ctggcatctc gagttcttaa tgcacgttaa tgaattccca
     2641 gaacttgatc aaaccctcag aaactttccg gtgccagtcg tgcttggtca tctaggctat
     2701 cttaaaacta gtcttggtgt taatgatcct ggctttcagg gtcttttatc actgatgcgc
     2761 gaggaacgcg cctgggtcaa actaaccgga ccctaccgaa tctcttcgca agatcgtccg
     2821 ccctattccg acaccaatcc atttgctgag gccttattgc gggcaaattc aaatcagatt
     2881 gtttggggaa gcgactggcc tcatgtcatg gtcaaaggat ttatgcccaa tgacgctgat
     2941 ctattcgatc tacttggcac ttgggttccg gaaccacaac tgcgcaagca ggtactgagt
     3001 actaatcccg ctcggctcta tcagttctaa gcgggaaaag aaaaagcccg ctattgcggg
     3061 cttttgctaa agcggctctg agttacagac ccatattttg ctgcgccacc attaagtaca
     3121 acatggggat agaaagaacg gtgttaatcc gtgaggtcaa catcgctgta cgagcagatt
     3181 tcgctttgag atcagcatca acaacaacaa taccgagtgc acgcttttga ttaggccaaa
     3241 taatgaacca tacattaaat gccatgatca gagcaatcca cattcccaag ccaattgcca
     3301 agaatggctg ttgtaaggta aatgctttat gtgcatagcc attcagaatg gccaaaatga
     3361 ttccggtcaa aacagttgcc attgcggcaa agcgaaacca gaataaagcg gtaggagcaa
     3421 ttactttacc aatcgctggc ttttgctcat cagggatctt tggcatcgat gggatttgca
     3481 caaagttgaa ataccacagc aaaccaatcc acataactcc tgaaatgaca tggaggtaac
     3541 ggaatacaaa tgcaacccat cctgggctac ccacattcga actaccaaaa agaacaataa
     3601 tcagcaagag caagacaaac cctgctaata ctgtacggcc aagagaggtc aagattgacg
     3661 ccatgtatat ctcctaaata ggtctaaaac cctgccacta taaaccatct gccccattta
     3721 tgccttgatg gacacatccc ttctggaagt attaagataa ttgccatgca aagactctat
     3781 atccaggaag tagtaacccg cgatggtttt caggctgaaa gcaacttcat acctaccgaa
     3841 gacaaggttc agcttctcaa ccgccttagt cgtgccggat acgccaaaat tgaagtgacc
     3901 tcctttacta gccctaaggc gatccctatg ctcgccgatg cagaagaggt gatgagtaag
     3961 attgagcgcg taccaggtgt ggagtacacc gttttgattc caaacctaaa gggtgcggag
     4021 cgcgccctac gagtcggtgt tgatgaattc aacttagtga tgtctgttag tgagacccac
     4081 aatcaatcta atctgcgtat gagtcgagcc gattctgcaa aagcgcttag tgatgttatt
     4141 cgtctagcgc atgacgctaa ggtggcggtc aatgtctccc tatctaccag ctttggttgt
     4201 ccgatgagtg gcatgacacc tatcccagaa ctgatgcact ggattgatca ttttgccaat
     4261 gagggtgtgc gcgggatcag catttgtgac accacaggaa tggcaaaccc ccagcatgtc
     4321 aaagaggtct gtgagctttc ccaaacaaaa tatccaacga tggaatggac tttacacttc
     4381 cataacacac ggggcatggg cctagcaaac gcacttgctg cggtagaggc cggcatcaat
     4441 cgttttgatt cttctttagg aggacttggg ggctgtccat acgcaccggg tgctaccggt
     4501 aatatttgta ccgaagagtt agttcatatg tttgatctaa tgggctataa caccaatatg
     4561 aacgtagatc ttcttttgga atgctccgca cagttgcgcg acctggttgg cagaacgctt
     4621 cccagccaac tattactagc tggtaaggtt gatcgcttgt acgataaggt ctgccaaaac
     4681 taatttattt cttggcagca gcttgcatct ttgccagata ctctgcaata cgatgtttga
     4741 tatcgggtac cgatcctgat tgaaccaagt cgttcaggtc cagtgctcgt gtatcccgtt
     4801 tgcaagcatt tagagccatc gctctggcat cgggagggat ctccaagata tgctctaaca
     4861 cttgagactc atatgcatgc cagtcggttg actccagtac agcagtcaat attccaaact
     4921 cttttgcctg atgagcatca atccgtcttg gattgccgat taactgttgc gctcgatctg
     4981 cgcccacaca ctctgcaagg cgacgtgatc ctaggatcag cccaaagcgt gcgcctggga
     5041 acatcatccg catatcttca gagcccactc gataacgaca agctagcaaa agatcagcgc
     5101 cggcaccaat tgtttgtcca tgggcaaatg ccatcgtatc gaatggggcg tgagcaatgg
     5161 cctgaagcat ttgctcaata cgtacgaagt gtaagagtag atcgcctgcg ctagcatcac
     5221 caaagtttga gaaatcaaag ccagcacaaa agccctgccc ctcccctttc agaatcaaca
     5281 gtcgggttcc atcaagctca gactccttag ttacctgtag cagttcattt gctaactcat
     5341 aacttattgc attgcgtttc tcaggccgat taagagtaac tcgtttgatg tgtccagaat
     5401 gctctactga aatggtgttc attgttttcc cactagttct tgattatgtt cacccaagcg
     5461 gggtggacgc ttttcaatgc ctgcagtttg tccattaaat ttaacagggt ttacaacggt
     5521 cttggtttga tgaccattcg ccatttctaa gggttgcacc cattccatgg ccgctgtttg
     5581 gggatcgctc agtacagcag agtactgatt aatcggacag caaggtattc ctgccttact
     5641 aaaggcttca atccagtatg cagcagtgtt cccttgcaaa attacttcaa tcaagtcttt
     5701 taggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn gcagaaccta gtttgaccgg gtctttacca accccaaaat actctgacgt
     5881 ttgtaaagcg gcaatcgcta atgaggttcc catcatgggt acatcgatat gaaccccctg
     5941 accgtttgct tggacttgcc gtagttgcgc acaaatattc atggcgccat atagacctgc
     6001 tgtgaagtcg gctactggta cgccacactt cactggcgca ccgcccgctt caccggtcac
     6061 actcataatg ccactcattg cttggatcgt gacatcaaaa ccaccttcat ttgcccttgg
     6121 gcctgtttga ccataggctg agatcgagca atacactaat ttagggttca gtttgagtgc
     6181 tgactcaaaa tcgagaccta agcgcttcat taccccaggg cgattgttct caatcaggac
     6241 atcggcttta gcaatcaact ctttcgcttt agcattgtcc tcggggtttt tgagatcaag
     6301 agtgattgaa cgcttattac gatttaagga tgcaaagttc tcactaaggc gatcgttctc
     6361 tggaccagaa tgcggaggcc aggcgcgtaa tgtatcgcca gagccagggt tctctaattt
     6421 aatgacatcc gcaccgagat cacccaacag cattgcacaa aatgggcctg cagctacatt
     6481 acaaatctcg atcacacgaa taccgtgcaa tgggcttaat acagatgcag tcatgggcat
     6541 attcatcggc attcttttta atggaatact ttattctaac gatcttagta aaggatctaa
     6601 gatttaggct ctgttcaaca tatcactcta cgatttaatg cataataaaa tgtattattt
     6661 attaagttct tagaaaatat gcctactagt ttacccactg attcgattgc accattacgt
     6721 tattttgtga cccaaatgac ccacctaatg gatcaatccc tagaagagtc ctttgtgcgc
     6781 cccaaagcca aagccctcct caaagagctt atttcgtcag atgactggtt acctgagttt
     6841 tgcacnnnnn nnnnnnnnnn nnnnnnnntg gggcccagga caagccaccc caattcatga
     6901 tcacatggtg tggggtctga ttggaatgtt acgtggctct gaaaaagggc aacgctatga
     6961 acgtgataac catggcaagt taacgcccat tggccatgaa gaaaccttaa tgccaggcga
     7021 tattgacgaa gtgagtcctg cgattgggga tattcatatt gtgaagaatg cgtttgccga
     7081 taaaacctcc attagcattc atgtctacgg tggcaatatt ggtggtgtga nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnntcat ccacacaagt gcccaacctc tgggataaat
     7201 ctgctgaaat cagagcgcaa ctgaactagt gtaatttgac gtgcggctct gtgcggcggg
     7261 taatcatccg cgctagcgtg tcaaggctga ccttgaccca accatgcaat gcaagctcgt
     7321 gcatcttata taaagagaga tacatcagct tagcaaaact accttcaatc atgagattgc
     7381 ccccaatcaa ccctcccatc atgctaccca ctgaactgta tttacccagt gagacgagag
     7441 aaccaaaatc ccgataacga taaggcttga gggctttatt ggccattcgt aactgaattt
     7501 gtttaaacag atgcgaggcc tgttgatggg cggcttgggc gcgcggcggc acaaacccgc
     7561 ccttcccttc gtttgcctct ggccatgggc aagcagcaca atctcccatg gcaaagatat
     7621 ttgaatcacg ggttgtttgt aatgttggca gaaccgctaa ctgattaata cggttggttt
     7681 caagaccagc aatctctttt aagaaatcag gggctttaac gcctgctgcc catactatta
     7741 actcagcagg taatctgctg ccatcggcaa atcgcacttc attaggtaca acctcggcta
     7801 ccttcttgtt ggtaattacc tctaccccca tgcgctctaa cagagtcatg gtggcactgg
     7861 ataagcgagg tggcaaagct ggcaagatac gtggggccgc ttcaatcaca ctgaccttta
     7921 aatctttatt cggatccact cggtctaggc cataagacac cacctcgcgc gtggtgcgat
     7981 ataactctgc tgccagctca acaccggtag cgccggcacc aataatggca acgtgtaatt
     8041 gatcggggga tagtggagta gtttgcgcct gcgcccgaat aatggcattg agcattttgg
     8101 tatgaaagtg cttggcatca ttttgcgact ccaagcgaag ggcaaattgt tctacgccgg
     8161 gcgttgcaaa atcattactg agactaccga tagagagaac tagtgtgtca taaggcactg
     8221 cacgctgtgg cgtgatctca attccttgat catcgtaata aggggcaaca atcacctctt
     8281 ggcgctcacg atccagaccg atcatctgac caatacggta tgtgaaatga tgccaatgtg
     8341 cttgggcaat gtaatcaacc tcgtgatcgc ccaaatccat actgccagca gcaatctcgt
     8401 gtagttttgg cttccagata tgggttcgat ttttatcgat tagcgtgatg gatagttttc
     8461 cagactttcc tttacgagga ccatagcgat cccccaatcg ggtagctaac tctaggccgc
     8521 ctgcgccacc gccaacaatt acgatccgat gcatatccga actttcactt tattattttt
     8581 attcgatgtg caagcactac tgtgcggtcc agccaccatc catattccat gccgcacctc
     8641 tgacttcgga ggcatcttgc ccgcacaaaa atacagccag agcagctaac tgttctggct
     8701 taacaaactc cccagacggt tgcttctcag agactaaggc gtgcttggcg gcatcattag
     8761 acatgttttc acgttcggcg cgtgcatcaa cttgtttttg aaccaacggt gttaataccc
     8821 acccagggca aatcgcatta caggtcacac ctgtatgagc ggtctctaac gctaccactt
     8881 tggtcaaacc gatgatgcca tgcttggcgg ctacataggc ggccttttgc atcgaagcca
     8941 ctaaaccatg gaccgaagca atattaatga tcctacccca gttgcgttgc ttcataccag
     9001 gtagcgctag tcgcgttgca tgaaatgctg agcttaaatt nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagcac atcaatactt
     9121 ccaaagcgct gctcacaggc ggcaatcatt tgtgcgatct cagcaggttt gctcatatca
     9181 gccccgtgat aatcgacttc tacgccataa gctttgacct cagccattgc agcgtcctta
     9241 tcaccaaagc cgttcatcat tacattggcg ccctgcttag ccattgcctt ggccattgcc
     9301 aaaccgatcc cgctagttga gcctgtgacc aatgctgttt taccttttaa ttgcatagtt
     9361 actttctgaa catgaatgga tgcactatgt tatttaactt ttcctagtgc tcgtaaaatc
     9421 ttctctgagg taatgggttg atcaaaaaca tgggtgctaa atggtgctag tgcatcatta
     9481 atagcattga cgactgcgcc aggggctcct gcagtcccgg cctcaccagc ccccttggct
     9541 cctaacttag acgatttggt tggggtctca acgtgagcca cctcaatatc tggcatctca
     9601 taagccattg gtaccaaata gtcagccata ttgccattgc gcatcagacc actctcatcg
     9661 tataagcatt cttcaaaaag tgctccgcca ataccttgaa cgatagcacc acgaacttgc
     9721 tcatctacca acattggatt gagaacgcgt ccgcaatctt caacagccca atgtttgagt
     9781 aactttacat atcctgtatc aacatcaacc tcaacgtaag aggcttgaac gccattcgta
     9841 aaaataaatg ggtagtcttt ttgagcgtaa tgacgggtaa ccattaaatc aactgaaaaa
     9901 tcggcaggta aggtatcagg cctaaagtag ccaattcgcc caagctcact taatgttaat
     9961 aattgctctc catttgcctt atcaacaata ttgccccttc gaactacaac cgaatccgca
    10021 tcccgcttca gaatagcgcc tgccaacttc aggatattgt cgcgcaatgc ttcgcaggag
    10081 cgaagcacgg cctctccccc aaccccagcg ccacgagatg cccaggttcc acccccatac
    10141 ggggttacat cggtatcgcc cgtaatgatc ctcacgttct ggatgggaac gccaacagca
    10201 tcagcagcaa tctgagcaaa aataccctca gtaccctgtc cctgttcacc aactcctatc
    10261 atgacggata tgattccggt agggtctaaa cgaacagttg ctccatcttg agatgcaatt
    10321 cgagccccgc ccacaccata aaaggcagcg cttggattgg taagctcaat caaggttgca
    10381 ataccaattc ctcgataaat accttttttt cgtaactctt cctgctcttt gcgaagggcg
    10441 ggatagttca tcatcttttc aatcttacgt aagcactgct catgcgacaa aacctccaac
    10501 ttaatcccag aaataccagt gtagggatac gcattatctg gaatgacatt gcgcttacga
    10561 atctcaatag ggtccatctt taacttttgg gcaaccaaat caactaaacc ctccgtaact
    10621 gcacaggcaa tgggatgccc aacgccacga tactggcagg taggagtttt attctgaaaa
    10681 acaactctta gatttgcacg gtagttctgg tgtttatatg gaccaccgac aagattaaca
    10741 acttgattgc cctcaattgc actagtccgt gggaacatgg aatacgggcc aatagcagtt
    10801 agatcatcaa tctcaaatgc caaaatatca ccttctttat tagcagcaat tctgccctta
    10861 atacggtgtt cgcgcgcatg aatatcgctg acaaaagact caagacgatc ggccacaaac
    10921 ttaatggggc gccccaataa aagagcaagg ccaaccgttg caaaatcatc tggataggca
    10981 tgaaccttaa taccaaagga gccaccaaca tctttacaga ttacatgaac gtctgattct
    11041 gcaaggccaa attgccggca atatagatct tgcatcatat ggggcgcttg ctgagagtga
    11101 taaaccgtta aacgcttctc gccaggatta aaatcagcga tttggcaacg cgtttctaag
    11161 gtaacgccgg tatgacgacc aaaaccaaaa gttgcttctg cggtgacatc cgcggattta
    11221 aaaacatcgt ctaccttacc gacatccagc gtacgagtaa agcataaatt gtcaccgagc
    11281 tctggatgaa tcacgggagt ggattgatct aaagccgact ccatgctaac tactgcgggc
    11341 aactcttccc actccacctc aacatgggct aaagcatcct ccgcttttgc acgagtttcc
    11401 gcaactactg caacaaccgg ctcaccttgc caacaggcac gatccactgc tagagcatat
    11461 tcaggagcag atttcattcc agctaaatgc ccaagggtcg ctacccaggg tttacaaatc
    11521 gtagccattt gcacgccatc aacgatggca ataacgccag gcatcttgcg cgccctatcg
    11581 gtatgaatct gcccaatttt catgtgtgct acaggcgaac gccaatacac aacatgcccc
    11641 attcttggga actgcaaatc gtcaatatag gtgccttgac cttgcaataa acgccttgct
    11701 gagtgacggg gctcactatg gccgatatag cgctgatcac ctgtaatagg atctaataca
    11761 aggccttgaa gatcggtagg cttattcata gattttctcc tgcatctgta tatacgatta
    11821 ttaagcggta gtaactggtt ttacttggcg accgtctgcg cgggcctcca gcacttccat
    11881 aatggcgtct acaatcgaat ggtagccagt acagcgacaa taatttcctg atatccactc
    11941 tcgaacttgc tcgcgattag acttaggttg atgctctata agttctgcag cggctagcaa
    12001 cattccggag gtgcaaaatc cacactgcaa ggcattatga cgcaggaatg cctcttgaag
    12061 atcattcaat acgccctgct cagtcaaacc ctctaccgtt tcaatcgtgg cgccatcggc
    12121 ttgcacagca agatataaac agccacgcac tatttgacca ttaacacgca ctgtgcacgc
    12181 accacaagcc ccttgctcgc acccaagatg cgggcccttt aaacctaaat cttctcgcaa
    12241 gaaatcgact aaatgacgtc tcggttcaat ttcggcactt atctgcttac cattaagcat
    12301 catgttgatt tgttttttat ggttcatggc aatctccact aaggcatcat ttttgttaag
    12361 gcgcgctcga gtaatacacc caataaatga gttttggttt cttgactatt ggttatatca
    12421 gccaaggtct caatttcctc gcgagccaga ttgacggcct gctgaatcaa tgcggcatcg
    12481 atagatttac cttcaataaa ggcttgtgct ttactagcca tcgttggaat tgctgcaacc
    12541 gagaaaaacg caaggcggca ggtttgcact ttacccttat cagttctaat catggcagct
    12601 aatccaacgg cagcgtaatc gccatgcctg cgcgctaatt cttgaaaact gtgtacttga
    12661 ttacttgaag ccaagggtat ttcagaggca actaaaattt cgtctgcctg caaggtagtg
    12721 gtgtacagat caataaagaa atctttagcg cttacttttc gtacaccatt aggaccctga
    12781 attaccatga tggcatccaa ggccaagctg caagccggcc actcagcggc aggatcgcca
    12841 taggcgagcg agccacccca tgtccctaaa ttacgaattg ctcgatgcgc aatatgaggc
    12901 gcggcctctt ttaaaagggg ggcgtgctgt gcaattaaat ctgaactctc aatttcagta
    12961 tgcgtgacaa gtgctcctat ccgaagcata ttaccttgta cagatatacc tttcagctca
    13021 gaaacggccg tgatatcaat caagatactg ggttcagata atcgcaaatt tagagtggcc
    13081 aataaggttt gtccaccggc aataagtcgg gaatcttcac catgctcact tagtaaggct
    13141 acagcctcaa ctaagttttt tgctttgaca taatcaaatg ctgctgcttt catgtgctct
    13201 ccttcactac tattcttttt tatcttctaa ttttgcatct tgtaatgtaa ttaactctcc
    13261 gcctaattgt ttggcaaaag ccaagaaaaa atcatcggca attttcttag ccgacgcact
    13321 aattaagcgg ccgccaattt gacctaattt tcctccgatt gacgcttcag tgctatagcg
    13381 tactaaggta ccgtcttccg taccaatcaa ctcaaccctc gccttacctt tcgcaaagcc
    13441 cgccgcaccg ccagtaccct caaaagccat tgaacaagaa acatcaggaa cgatgtcaga
    13501 taacagtagt tttcctgcaa accgagctcg caccggccca attttgaaca ttaccttagc
    13561 cctgacttcc tcatcagaaa tacgctctat ttcttcgcaa ccggggatcg aggcgtggag
    13621 agtgcttaca tcatttaagc ctaaccaaac cttgttaact ggcgcggcta ttaactgctc
    13681 gccatttaat tccattccga ctccttcggg ttaatacgga tcattcttaa ctcatctaat
    13741 aaaaataagg gtttaaggca atttacaaag agttttatgc aacaaaatgg cgtttgctct
    13801 tcgtaaagtt ctagaataac ctctaataat gtaatgatac tttttaaaga ggaattgcta
    13861 tgtctaagtt atcactgaac catttttcga ttcggagtct cgatttagat aaaactgttc
    13921 aatttttttc agatgtgctg gatctaaaac cagggccaag accaaatttt ccatttcctg
    13981 gggcttggct ttacagcgga gacagctctg actatgccaa ttcagtagtg catattatct
    14041 ctatcgataa aaatgatcct gctggattaa aacagtatct cggtgatcgc gatgccacct
    14101 ctttacaggg ctccggggct gttgatcata ttgctttctt tgccaatggc cttgaagaaa
    14161 tgatgtccca cttaaagaaa ctagacgtcc cattccgaga acgcactgtt ccaagcctta
    14221 atttgcacca gatctttgtg gatgacccta atggaattgt gatcgaactt aactatccag
    14281 ccaacgaaaa ggctgcttaa tttattactg tggcaaagat acttgttgcg ggtggttcgc
    14341 taggtggctt attggttgcc aatttattac atcgcagcgg tcatcttgtt accgttctag
    14401 aaaaatcagt tggctccatg gacgggcgtg gagctggtat tgttacccac gaggctttgc
    14461 gagcaacttt aaaggaatgt ggggcgttgg tcgatgaggc cttgggcgtt tatgtttcta
    14521 aacgagttac tttagcgagt gatggcacat gtcttggcca agcctccatc ccccaaatac
    14581 tgacatcctg gagtaggctt tatcaaattc taaagacttg ttttccagaa actttgtatt
    14641 tacaaggcaa agtaatttct aagcttaaac aaaatactga tgaggtcatg gttgagtgcg
    14701 aggatggaac gcaatacaaa ggagatcttc taatcgcatc ggatggcatt cgttcttcgg
    14761 tcagaaattt actaacgcaa catgttcaac caaaatacgc tggttatatc gcttggcgtg
    14821 gggtgtgtga tgaaaaatta ctgagccaat atacgcttga tacattattt aattattttg
    14881 gtttttgttt acccacaggc gagcaaatgc tgggctatcc ggtagccggt tctggcaatg
    14941 acacgcgagt tggtcatcgg cgttttaact ttgtttggta tcgacccaca aagaatcaag
    15001 aggagttaac tagcttatta agtgatgaag cgggaaattt ctacccaaat ggtattccac
    15061 ccgccttggt tgccaaaaaa catattgatt ctatgcgcac cgcatcaaag atgaacttgg
    15121 cgccccaatt tgctgaggtt attgaaaaga cagtacagcc gtttttccag cctatttacg
    15181 atgtttactc agagcaaatc cactttggtc gtgtagcgct aatgggtgat gcatcgtttg
    15241 ttgcaagacc ccacgtcggt atgggcgtca ctaaagcagc cgaagatgct atatccatta
    15301 atcgacatat tctggcactt ggtgctaacc ctaatgccct cagaggctac caattagagc
    15361 ggctacccat aggacaaaaa attattcgcc atgctcaatt tttgggtaga tatatgcaat
    15421 cacaaggtct tattggaagc gggcatcagc ctagcctcct tcgaaatccc aatactgtca
    15481 tggttgaaac tgccttggga ttggataaag cctcagaaga aagtattaag atctagccaa
    15541 taagcgaaaa gcttagtaaa gtagttttgt atttaaaaaa ggagactgta atgagtaaaa
    15601 aaacaaatca aacacgccga aatattgtga ttgccggtgc cgcagcggca agttcaccat
    15661 tttggttcaa catagctacc gctcagtcaa gcacaattaa gattggtttt ccggttccgc
    15721 tgactggccc cttttctgca gaagcccaag atcaagttcg cgctgctgaa cttgcaatta
    15781 aagaatttaa tgatgccggt ggatttaatg gtcgaaaagc tgaattactt gtgcgtgatg
    15841 acaagcttaa ccctggcgaa gctgctaccc gtactctaga attaattgaa aaagataagg
    15901 ttgattatgt ggtcggctct ctttctgcag ctactcaatt atcgattaac gccgtctgta
    15961 aagagcgaaa ggttatcttc aattcaatta gccaatccga tgctatcaat gaagtaaaag
    16021 attggtcgat ttacacgttt catgaagcct taaatccgca tatgacggct ggtgcggtag
    16081 cgcgctatgc attccctaag tttggcaaaa agattgttta cctaacagct gactatgcct
    16141 atggtcatga gatggtccga ggctttgaac gggctggtaa gtcactaggt gcaacaactc
    16201 ttgctgatat tcgtcaccca ttgggaacat ctgactactc tgctttcttg ccacgcatta
    16261 aagcactcaa tccagatatc ttggtcttgt gcaacttcgg ccgtgacctc gttaattcag
    16321 caaaacagtg cactgacttt ggacttaagt cgagcatgaa gatcatcaca cctgtactcc
    16381 tctacacagc tcgtcaggct ggcggccctg aagcatttga aggtatcctt ggtggaactt
    16441 cctattactg ggggatggaa gatcgtgtac ctagcgcaaa agcctttaat gataagttcc
    16501 gcaaagcgta caacgggatt gtgccatcag actacggcgc cttgggctat gctggtatct
    16561 tgagcgtctt acaagcagtc aagaatgcta agtctactga tactccaagg gtcattgctg
    16621 ctatggagca actcaagtat gactggtaca aaggtcctca gttctatcgc aaatgtgatc
    16681 atcagtctgt tcaatcggtc atcgttattg aatctaaatc caagaatatg aaagataaga
    16741 atgatgtctt taatgttctt gcaattgatg ggccagatga aaagaccctt cgaagctgcg
    16801 acgagttagg ccacaaagcg taaccatcct gtacttcaac tatacccgcc tgctccttaa
    16861 gggcgggtag ttttttgggt taatttatga ctggtcttac tctcgagctt ctgggcatgc
    16921 aattactaac aggcgttgcg cttggaagta tttatgctct attagcactc ggtctgtgtc
    16981 taatttttgg aatgcttgga gttgtcaatt tcgcccatgg ggcatttttt atggttggcg
    17041 cattcctagg ggtctatttt ttgggtctca caggtaattt tttctttagc cttctcttgg
    17101 tgccaattgt gactggcgta atcggacttg ttacagaacg tttcttaatt cgaccgcttt
    17161 atggtcgagg tattgattat cctcttttgc tcacgtttgg tctttcgtat gtcctaattg
    17221 aagtcatgcg tgtcacattt ggtattgagg gcttaccgtc aataaccccc gacagcttaa
    17281 aaggttcgat gaatgtgggg attggttact ttccaaaata tcgcatcttt ttaattgcaa
    17341 tgaccgcact gattattctg ggtatctggt tctttatcca aaaaacacgt tacggcttaa
    17401 tcattaaagc gggctcacgt gatcctgaaa ttgtgaaagt attaggcgtc gatgttgcaa
    17461 aagtttggtt gatggtattt ggcattggta ctgctattgc aggtctttct ggaatccttg
    17521 cttccccaac acgttctgtt aatccagaaa tgggaattcc tattcttgca gaatccttcg
    17581 tagtcaccgt agttggtgga atgggttctc cgattggcgc agttgtggct ggcctgctcg
    17641 ttggagtggt ctacagccta acttcgcttt tcgccccagc tctagccgag ctgtcgattt
    17701 ttgtgctgat ggcagtagtt ctgctaatcc gcccacaagg tttgtttggt aaatctggct
    17761 ttatgagtta agcattcatc atgcaagcta tatttcaact aattgccact caccgagttt
    17821 tggccactac cttattttta atggtatttc catggttaat gccctatgaa gctctagcaa
    17881 tcaatattct gatctttggc ttattcgcta tgggctttaa tcttttattt ggttacatgg
    17941 gtcttttatc ttttgggcat gctgctttct taggaatggg tagttacatt accgggatca
    18001 gtattgttag ctacggcatg ccatggggtg cagcaatttt atttggtgtg attgcagctg
    18061 cttttggtgg cttagttatt ggctttttag caattcgtac tcggggcatt tatttttcaa
    18121 tggtaactct ggccttggga caaattgtct attacgggtt ttataaagcc gagaagttga
    18181 ctgggggtga aaatggactg aggggtgtga aagtggatcc atttaatatc cttggtttta
    18241 aagtcgactt tttgaatcct ttagtaaagt actacatcat tcttttcttt gtggtgattg
    18301 cgatttggct ggtttccaga atcctaaaat cacctttagg cgccgttatg gaagctattc
    18361 gtgagaatga aaaaagggtc ttggcatgcg gctacaacgt tgcccaaact aaattgttag
    18421 tcttcatctt gtcagcggcc atctgtggtt tagctggctc actacgcgcc cttcatttat
    18481 ctattgtgcc aattgattca ttgcattact tgcagtctgg tcaagcggtg atgatgagta
    18541 tcttgggtgg aatggggact ttctttggcc cctttgttgg tgctgcggtg atgctatatc
    18601 tcgaggatgt cgtgactagc ttcacaaaac actggatggc tgtggttgga ttaattttta
    18661 tgttctttgt gctatttttc ccgaaaggaa tttggggaag tattcttgag cgtcttggta
    18721 gtcaaaaagg tgcatcaaaa tgaataagtt tggcaccacc cctattcttg aggctcgtca
    18781 cgtatccat
//
LOCUS       DDPQ01000010           11131 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_8102,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000010 DDPQ01000000
VERSION     DDPQ01000010.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11131
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_8102"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    10468..10501
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10897..10970
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3032 a         2692 c         2405 g         2892 t
ORIGIN      
        1 caccctacaa agttcggccg actatcttct gccagtccct cttggggttc gcaaattaac
       61 aaaacgaggg tttaatcaaa gctgggagat tgcgaaacat attcagctac ctaaaaccgt
      121 tcgcaagata cccaatgcct taggtcgcat cgacgatgag acatcgcaag cgcagcggaa
      181 tcgagagatg cgcaaagaga tggcacagga cttgttctac ctaaacacag attggtccgg
      241 gcgctttaag aaccaaagaa ttattgtttt tgatgatgta atgaccactg gggcaacgat
      301 gaatgcaatc gcatccttat taaaaaatca tggcgctaaa catgtaagcg cttgggttgt
      361 gttgcgaact ctacccaaga gtgatccatt tgcaatatga tcgcaacatg tttaatgttg
      421 ttttatttga acccgagatt ccgccaaaca cggggaatat tattcgcctc tgtgccaata
      481 ctggagcaag acttcatcta attgaaccgc ttggctttcc gctcgaacac gccaagttaa
      541 ggagggcggg gttggactat cacgagtttg caaacattat ggtttatcaa agctggactg
      601 attttctaga gaagagtaaa gcaaatctat cgcgtgtatt tgccttaaca acgaaagggc
      661 ggtcatccct gttaaccagc aaatttctta aagatgatta ttttgtcttt ggctcagaaa
      721 caaaaggggt tagtgctgag gtaagagcgg ctattcctaa agacaatcat ttacgactgc
      781 ccatgctacc tgcaagtcga agtctcaatc ttgcaaactc ggttgcgatt gtggtctatg
      841 aggcgtggag gcaacaccaa tttactggag gtctttaaga ctcggccttg ggttcgcgtc
      901 ccatgagagc ctgcacgcct tgctcaacgg ttaatttgcc atttaaaacc tccacaacgg
      961 tggcacaaat tggcatttct acctgaaggc gctttgctaa atcagtcact gctttagcgc
     1021 acaagacacc ctctgccaca tggcctaatg cgtgtaaaac gtgttctaga tcttggcccg
     1081 ctgccaagga caagccaacg cgccgattac gtgataaatc accggttgcg gtcaaaatta
     1141 aatcgcctaa accagttaag cccatgcagg tctcactctt accgccggct gccttggtta
     1201 aacgcatcat ttctgctaag ccacgtgtta gtaatgcagc ccgtgcattc attcccaagc
     1261 ctagtccatc accaattcct gcggcaatcg cgaggacgtt cttaatcgcg ccgcctagct
     1321 ccactccaat taaatcatcg ctggcatata cacgaatatt tccatgatga aatacctgct
     1381 ggactaattc acagaactct ttttgatggc tggcgaccgt tagcgcacac ggcaaacctt
     1441 caccgacctc tcgcgcaaag ctcgggcctg acaaaactcc aaaagcatgc gcatggcttg
     1501 gatgctttgc tagctcacgc tgtaatactt gatgcggcaa taaggctgta ttgggctcta
     1561 gtcctttgca taggtacaaa atattgagcg gatgtttcgc ttgtatcaat acatccccta
     1621 agatttggga caacccagac attggtgtag caataaccaa taggtcgcct aagcgcaatc
     1681 tgcccagagc ttttagaaaa tcagcctcta atagaagttc cgtagggagc gaaataccgg
     1741 gtaaatattg tttgttctct gcatgaattt gcagtgcctg aatctgttgt ggatcacgcg
     1801 cccataaaca gaccgaatga ggggcatgat ggcgggctgc tgatagggcg attgccgtac
     1861 cccatgctcc accaccaaga accgtgatat tcattagttg ggcaggataa tcttgctttc
     1921 tgcgtcacca gaacccgctt gagcaccttg cgcggcttgg gcttgcgcca agcgattctc
     1981 atacatgcca tgaaagttaa tatccgagag atgaatgggt tgaaagcctg cgcggctaat
     2041 aatatcggca acgtttgagc gcaaatatgg gaagattatt gttgggcatg taataccaag
     2101 catcgggtca atctgctctg gtggaatatt tttgaactca aaaatacctg cttgttttgc
     2161 ctcaaccaag aacataacct taccatctac cctcgctgta actgtgctca gtagcgcaac
     2221 ctcaaataac tcatcggtca aacgatctac tgcaatatca agctctacct gaatttgtgg
     2281 ttcgcttgcg accaacaaaa tgcctggtgc attaggctgt tctaaagata aatcttttaa
     2341 ataaacgcgt tgaatcacaa aggaggggtc attagtaccc gttgcagaat taggtgcagc
     2401 atcactcatg ttaaatctct ttctctagat aagatgggag taagaactaa aaattatcct
     2461 agcaggggat caagcccgcc tgcgcgatct aatgctgcaa gatcgtcata accgccaatg
     2521 tggtgatcac caatatagat ctgtggaact gttctgcgac ccgtacgggt catcatttct
     2581 tcacgccgac tggggtcgac atcaatcaac actttctgaa gattcagtac accttttttt
     2641 tgtaagagct tttctgccat tacgcagtat gggcagacac gggtgctata catcaatact
     2701 tcaggcataa ggactccgtc tacttgatca aaggcaaacc ggaggtctgc caggcaccaa
     2761 ttccaccgtc taaaacgccc acctcagcaa atcctaattt tcgtaattct ttggcagcct
     2821 tttgggaatg cgctgatcca cgaccaatca atactactgg caatttctga tccaatttca
     2881 aagccttcag gccggcatca aggctctcca aggctagatt tttagcattg ggcaaatggc
     2941 ctgccttaaa atcctcctcc gagcgcaaat caatcacatt tgctttgcga cggtttatcc
     3001 aaatcgtcgc atcggtaggg cttagaccct tgccagtcat tagggttgat aatgtcggca
     3061 tgaatagagc catacctgaa atgatcatta aggcggcgag tactaaattg tctgtttgag
     3121 ctaagaagtc cattgctaga ttatagaatg catctatgaa acaactcatc cttgtgcgcc
     3181 atggccaatc tgcgtggaac cttgaaaatc ggtttacggg ctgggccgat atcgatttaa
     3241 cccccaccgg cgttgagcag tccctgaatg ccggaaagct tttaagggag gcgggttacg
     3301 aatttgatat cgcctatacc tcggttctca aaagagcaat tcggaccctc tggcatatcc
     3361 aagactccat ggatctaatg tgggtgccgg tggtacacag ctggcggctt aacgaacggc
     3421 attatggcgg tcttaccggt cttaataagg cagagaccgc agctcaattt ggggatgagc
     3481 aggtccatat ctggcgccgc tcttatgaaa ttccaccccc accgctaaac caagaacagc
     3541 gcgcagaaat tgccaaagac ccccgctatt ccaagctaaa cgagcaagac atcccccaag
     3601 gggaatgcct aaaagatacc gtgcagcgcg ttttgcccct atggaatgaa tctattgctc
     3661 cagccctaaa gctaggtaaa agggttatct tggttgctca tggtaatagt attcgcgctc
     3721 tgatcaagca tctagaccaa gtctccgatg aggccatcat gggcattaat gtccccaatg
     3781 gcaatccctt gatctatgag ctagacgatg aactaaaacc acaacaacat ttctatttag
     3841 aggcaaaata ggggcatgcg ccagctattt agaaatattg ccctcatagg aatcggatta
     3901 gtcgccggga ttgccgcgac cattcaactc tctgctactg ctcaacaggg aactaatacc
     3961 cttcctttag atgagctccg gacactctcg aatgtctttg ggcaaattaa acgcgagtat
     4021 gtagagccca ttgaggataa gcaattgtta actgatgcgg tcaagggcat ggtcagcagt
     4081 cttgatccac actccgccta tttggataaa aaagattttt ctgagatgca agagcaaact
     4141 gctggcaagt ttgcgggtct cggtattgag attacctcgg aagacggttt ggtcaaggtg
     4201 cttaatccga ttgaaggatc gcccgctgca aaggcaggcc tccaagcagg tgatctaatc
     4261 acgcgcttag acgataagcc tgtccgtggc atgtctttag ataaagcagt gcgcactatg
     4321 cgcggcaccc caggtaccaa aattaccctc acgattttcc gcaagagtga agaacgcacc
     4381 ttcccggtga cgattacacg tgcagaaatt aaagtgcaat cgatcaaagc aaaaattgtg
     4441 gataacaata ttgcatgggt tcgtattacg agcttccaag aacgcaccat tcctgatctt
     4501 gctaaaaaat tagctgagct tgccaataaa gatccaaagc taaaaggttt ggtgctcgat
     4561 ctgcgcaata atggcggcgg tctgctgcaa ggggctgttg gagtggcggc ggctttcttg
     4621 cccccaggtg cagtcattgt ttctacaaaa ggacaggctc ctgactccaa acaggtattt
     4681 aatgcagtgc ctgccatgta taagctgaat gagtccggcg accctctcga tgaggttcca
     4741 cctctcttca aaaaaatacc gatggtcgtt ctggtgaacg cttattctgc atctgcttct
     4801 gagatcgttg caggtgcctt gcaagacttc aagcgcgcca ccatcattgg caagaccacc
     4861 tttggtaagg gctctgtgca aaccgttaga ccgcttagca atgactctgc actcaaaatt
     4921 acgacagcct attactacac gcccaatggt cgatcgattc aggcatttgg aattaaaccg
     4981 gatattccgg ttgatcagaa taaagatggc gatcctgatg atgtgttaat cactcgtgaa
     5041 atcgatagtg aaaaacattt acgcaataag caatcttcag aagaagcatt aattaaagac
     5101 cgtgagaagc gccgccttca agagctgcag ctaattgaag agcgtaacgc caagaagacg
     5161 cctgaagaac gagaaaaaga taaaagtaag cgccctcctg agtttggaag caacgatgat
     5221 tttatgttgc aacaggctgt tgcttttctg agcggtcaaa ctgtgaagcg ctcagcatcc
     5281 aagcttgaat agttctcttt gctcatgaat gacgagcaat tactacgata ctctaggcac
     5341 cttctgctcg aagagattga tattgcgggt caagagcaat tactcaatag ccatgtttta
     5401 gtgattggtg caggcggctt aggctctgca tgtgctccct atttagttgc agcaggagtg
     5461 ggtcaaatta ccctagtgga tcacgatcgg gttgagctta ctaacttaca gcgtcagatc
     5521 atgcatcaaa acaacagtgt tggcatgact aaggttgggt ctggaaaacg atttctaaat
     5581 agccttaatc ctgggatcat cattaatgcc atcgaacaaa aagccgatga agcattactt
     5641 gaagagcttg tcataaaggc atcggtggtg attgattgca ccgataattt tgcgacccgt
     5701 catctcatta atcgagccgc cttcaaacat aagaaagtac tggtcttagg agcggccatt
     5761 ggatttgacg ctcaagtcag tgtatttgat tttaggcaac cgagctcacc ctgttatgcc
     5821 tgtgttttcc caccagaccc caattttgct gaaactaatt gcgccagtat gggcgtcttc
     5881 tctccactcg ttggaattat tggctcgctc caggccgcac aagccttaca aatcttgatt
     5941 ggttttggtg agcctctcct ggggcgacta ctcatttgga atgcccgtaa ttcgcaaatc
     6001 gatgaaatca agatacgacg aaatcccgat tgcccggtat gcggggctca ctgtcaataa
     6061 gtgttagtgg attgaatcta ggcgattaag tatttcgctt tgttctacag gatcaaaaga
     6121 ggctaagagc tcatcaatat gctttttcag ctttcgtgta tccgcttgca aaatctcacg
     6181 cttaactagt agcaattgac taaagtgcat tgagaaatca gtaagcccca ttgccaataa
     6241 aagcttagtt agcgctggat ctcctgccat ctccccgcat actgcaattg gtacgttcgc
     6301 cttttgggcc tgccgaatga ccgaagcaat caaatttagt accgctggat ttaatggatc
     6361 atatagatga gcgaccgcat gatcagctcg atcgattgcc aaggtgtact gaattaagtc
     6421 attagtacca atcgataaat aatcaaaatg ttttacaaag agaggcatca tcaaagcagc
     6481 cgctggtacc tcaatcatgg cgcccacttc aatatttgga ttaaaagcct gaccacgttc
     6541 gcgaagctgt tgttttgctt tatcaatcaa gcgaagactt tcataaatct cttgagcgtg
     6601 cgctaacatt ggcaataaga gtcgcacctt accgtgagcc gatgcccgca aaatagcgcg
     6661 cagttgggtt aaaaaaatct caggctctga taatgaccac cgaattgctc ttaaacctaa
     6721 cggtgacgtg cccgtactcc cggtctccgc tccggacacg gatttatcag agccaatatc
     6781 aatcgttcga atatttacgg gcaagccaaa catagcctca acagcacgcg tatattcttg
     6841 atattgcttt tcttcatcag gcaggccgta ctgttggttc ataaataaga actctgaacg
     6901 gaataagccg atgcccacag agcctaattc aattgcgtgt ctagtatctt cgggtaactc
     6961 gatattggca aagagttcaa tatcaacacc gtcttttgtt ttggtcggac tatatttgag
     7021 ctcttgtaat ttttgggtct tacgatattg ttcagattgc aaagtgcggt attcacttag
     7081 cagagcctca tcgggcgcca caatcacaat ccccgcatta ccatcaataa ttaaccaatc
     7141 accgtgctcg ataatgtcac tcgcatggcg gatgccgacc agtgccggaa tctctaaact
     7201 gcgtgccaca attgcggtgt gtgaggtctt cccccctaaa tccgttacaa atccgcttaa
     7261 gtcgttctcc ttaaaacgca acatatcgtg aggtgcaatg tcatgagcca ccacaattgt
     7321 tccatcgtca aagtcaccgg agattaaacc ttcacctccg atcactgttt gacgattttg
     7381 cgaatggatt gcctttataa cgcgttctgc aacctgccga atatcattgc cccgctcttt
     7441 aagatacgga tcctcaatct ccgaaaattg ctcaagcaga tcatttaact ctgtggttaa
     7501 ggcccacgca gcatttaaac gcttttgacg aatcaactcg agtggctttt gtgccaaggt
     7561 tgggtctgac aaaatcattt gatgaacatc taaaaatgca gccatctctt gaggggcgtc
     7621 ttttggcaat gtagtcttta gagcactcaa ctcattactg acctgctcaa atgcttgcaa
     7681 tagtttgttt gcctcgagct cttcggttcc tggctcaact aaataatggc taacctcgag
     7741 tgccgcccgc gaaatcagta ctgctttacc aatagcaatc ccttttgaaa ctgatacgcc
     7801 atggagtgca aaactcatgc nngctttatc ctcttgctcg ccaacggttt ctagcgttac
     7861 gacacttcca attccggcgg ccaacatcat cacccccata atacttttag cattaatccg
     7921 gcgcccgttc ttgctcagaa atacttcaca aggatattca ctcgccaatt gagaaagctt
     7981 ggccgaggcg cgtgcatgca aacctaattt attgatgatc tcaatatcga cctttggcat
     8041 tatggatccc caaccttttc atttacagag gtttctacat gagtaacagg tgtccccaac
     8101 cgaacaatac cgttttgtcc cccttgcatc gccttaagag caacggattc caagccatcg
     8161 cctcgatagg aaattgctcg cattagcatt ggcaaattga taccggctaa gaccaaaatc
     8221 ttgcaatcta ataaacccgg ttttgttaaa cgggacgcca cattagcggg agtagcgccc
     8281 attaaatccg ttaagaccaa aatgccagcc gcacttttga cttgcgttgc cgctgtcatt
     8341 accctctctt cactcatgcg aacatcttcg tgaggtggaa tatcgactgc tgtaacccga
     8401 acgggcagct caccataagt gtgctcaata aaccctaaca tagaacttgc aatcggcgta
     8461 tgcgccacaa ttaagatacc gggcatgatt acgctaactc cttttcaagt cgtgttaacc
     8521 atttttttgc aacatcgcaa tcggtttgtt gagcgatttc tacaaaacaa gttgggctag
     8581 ttacattaat ctctgttaaa tatcccccaa tcatgtctag gcccaccaaa aataatccgc
     8641 gcttcgctaa tatgggtgca agatgatttg ctatctcttc ttcacgggct gtcagcgcca
     8701 tggctaaacc cttgccgccc actgctagat tgccacgtat ctctgttcct tgcggaattc
     8761 tggctaatgc ataaggcatt acctcgccac caattaagag aacccgcttg tctccggctg
     8821 taatttctgg gagaaatcgt tgcgccatta aagtgcgcga accattttca cccagtgtct
     8881 caacaatact tcctagattt agaccatcgg gtcccacccg aaatacgccc atgccaccca
     8941 tgccatctaa tggtttaata acgatatcgt gatgtagtcc atgaaaatcc cgtatcgcat
     9001 gaatatcccg cgttaccaag gtggggggaa taaattgagg aaactcagta atggataact
     9061 tttctgaatg attacgaaca gcatctggat tgttaaacac ccgcgcaccc tgcagcactg
     9121 ctgctgacaa tatccaagtg cttgttaaat actcgaccgt gaacggtgga tcttggcgca
     9181 tcaagaccgc agtaaagtaa tctaaagagt gttcttcaac ttcgcctaat cgataccaaa
     9241 tcagatcgtt cgttactgtt agaggctgag cccgcgccct ggctttgcct tgaccccaca
     9301 ataaatcatg gctttgtgta taccaaattt catgcccggc actttgagcg acacgcatca
     9361 tggctaaggt ggtatctttt tctaccttaa aagaatcaag cgggtcggca ataaaaagaa
     9421 gtttcatggc aatggccaat actaggcagc ctctgcgtct ggatcggttc gctctagctc
     9481 aagtgaggca gctagcaagg ctaagcgggc caccacgcca tataaataga aacgattagg
     9541 tgcagcactt ccgggctttg cccgcatgtc tggcattgca ttttgttcaa atgccaatgg
     9601 cacaaaatgc ataccggggg cattcaagtt ctcatccggg ccacgatccg tatgtacgcg
     9661 atagaagccg cccaccacat agcgatcaat catataaacc acgggttcag cgactgcttc
     9721 attcaccttc tcaaaggtat acacaccttc ttgtacgatc acatcactaa cctctagacc
     9781 ttctttaacc acgctcattt tgttacgatc gcgtcgattt aaattcttca gatcatttgc
     9841 atcatgggca atcatgaccc ccatgccata ggttccagca tcgggtttca caacaacata
     9901 aggcttatct tttattccat actcgcgata cttcttagca gttttcttga gtacttgatc
     9961 aaccgctgcc attaattgtt cttcaccctt ctgctcatga aagttcattc ctgaacagcg
    10021 agcaaaatag gggttaatca tccagggatc aatattaatt aatttggcaa atttcttggc
    10081 aagctcttca taggccaaga aatgattgga cttacgtcgt attggccagc cagcatgcaa
    10141 accgggcaat aaatattgct catggacatt ttctaaaatg ggtggaatgc ctgctgataa
    10201 atcattattg agcaggattg agcaaggatc aaagtctttt aagcccaaac gcgttttgcg
    10261 caaacctaaa cgggtcagag gctctaaaac taaacgtccc ccctctggta aatcaatcgc
    10321 agtcggtttc ttaatttcat ccgataaagt gcccaagcga acattaagac cagcttgtcg
    10381 cagaatcgat gacaaacaag cgatgttttg caaatagaaa gtattgcggg tatggcgttc
    10441 ggggattagt aataggtttt tagcttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 naggttatta aagccgcccg ggaagagatt ggtatcaacg ggggataatt taaagccaga
    10561 gttacgcaaa tcaaccgagc aataaaaggg cggggtatgt tcctgccact ccagcctaaa
    10621 ccagcgctct attgtcggag ttgcctcgag tactttggac tcaagctcta gaagggggcc
    10681 actaagggcg gtaataagat gcgggaccat atccgcattg taagtttttt tgaaagaaag
    10741 acgcggaatc tctcgattcc gcgcttaaaa actaggcaac caaccagtgc cgcctagtga
    10801 ggggtaaaaa taaactgact aaatcatgct tgcaacgctt aaatctctaa ttagtctggg
    10861 tttgccggaa tcaccaaaaa ccaacaaacc cattccnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaggcaataa
    10981 acgctacaac gccagaccag atcaatgtca caacgacacc ttgagcttgg acgaccactt
    11041 ggcttgcaat ggagtaatcc tttgctactg catttgcaac gtagtcatat atgccagcac
    11101 caccaagggc aggatcagca aatacaccgg t
//
LOCUS       DDPQ01000011            6887 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_19230,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000011 DDPQ01000000
VERSION     DDPQ01000011.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_19230"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2122..2136
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1899 a         1624 c         1475 g         1872 t
ORIGIN      
        1 acaatcgccc gcgaacgatg gtcggtaatg ggtaattttt ctagatgatg atctaagtga
       61 cgctctactt gcttttctgt ttcagccacg aatcctaaac tccaacggtc tccagcaagc
      121 cctgccaaaa aacccattcc aaaggatcca aaataccaaa tggggtttaa taaactgggc
      181 ctcgaatcta gctctttcaa acgctcttca caccaggcca tatggtccat ttcttcatgc
      241 gccgctagat ccaaaagagc tcgtgttttg gggtcttttg ctagcaacct ttgcgactga
      301 taaagggctt gagcgcaaac ctcgccaaca tgattaaccc gcattaaacc agcagaatga
      361 gcacgctcat cagggcccat atcactatca gaagtaagta cttgcttatt atttagatca
      421 ttttctaatt gggttttagg aatgggtctt cggtaatggg cgccccctgc aatcgaacgt
      481 aaagcagtat caaactccga aatgagttga tcgatggggc ttctattttt acctggaaaa
      541 ctgcttgtca tacccgcact tcagtcttac aactttcttt ggttttgaga cccagttcgg
      601 taagtaattg acgatctgct tcggcctctg gattaccagt tgttagtaac tgatcgccat
      661 aaaaaataga atttgcgccc gcctgaaaac acatggcttg aatcgccttg ccgagttcct
      721 gacgtcctgc tgataaacga acccttgctc ttggcatcgt gattcgggct accgcgatag
      781 tgcgaacaaa ttcaaggggg tctaacttgg cttgggattc aaggggagtg ccagccacag
      841 gcactaaatg attaatggga accgattctg ggtagggctc aagattagct aagcgagcta
      901 taaatgaaac ccgttgctcc cgactttcgc ccataccgac aatcccccca caacagacag
      961 acattcctac atcgcgcaca tgctgaagag tatctagtcg atcttgatag tttctggttt
     1021 gaatgacatt ggggtaaaaa tcttcactgg tatctaagtt atggttataa aaatcaaggc
     1081 ccgcatcttt cagggactta gcctgagagg gctcgagcat tcccaaggtg gcgcaggttt
     1141 ctaggccaag agcttttacg cccttaatca tctcaactac cttttccaca tcacggtcct
     1201 tgggttcgcg ccaagcagcc cccatacaaa aacgattgga gcccgctgcc ttagctgcct
     1261 ttgcagcctc caacacttcc tctaaatcca ataacttagt ggcttttacg tctgtatggt
     1321 agcgagccgc ttgagggcaa tagccacagt cctcgggaca gcccccggtc ttaattgata
     1381 aaagcgtagc cagctcgaca tcaccctctg gaaaatactg acggtgggtc tcttgagcct
     1441 ggaacatgag ttcattaaat ggaagatcgt ataaagccgc cacctgctcg agggtccatg
     1501 ccactctgac ggcgggagct cttggtttta cctgaacgag gggggctgtg gtttggtggg
     1561 ctgacatcgt tttctatgac ttaatctcta aaaccaataa cataacagat ataagactaa
     1621 atccctcaat gagtgttcca ataagcctat gaatagcccc tccaattctg tggattgggt
     1681 tgcacgaagc cttgcttcgg tatggcatcc ctgtacccaa atgaaacatc acgaagctta
     1741 tcccttggtg gcgattcatc atggggaggg tccgtggttg attgatgccg caggcaatcg
     1801 ttttcttgat tgcattagtt cctggtggac caatttattt ggtcatgcta atccaaaaat
     1861 taatcaagcc atcattgaac aacttaatac cattgagcac gttatgttag cgggcttcac
     1921 acatcaaccc gtcattgaac tatctgaacg tttgagtcaa ttaagccagg ggcatttagg
     1981 ccatgccttt tatgccagcg atggtgcgtc tgctgttgaa atcgctttaa aaatgagtca
     2041 tcacttctgg aaaattcagg gcaagtctgc aaaaaagaaa tttatttgtt tacaaaatag
     2101 ttatcacggt gagaccttgg gnnnnnnnnn nnnnnngaaa gtgctgagga tatcgctaaa
     2161 cgagctattg cggaccttga gctgtgttta caaaaaaatc acactgagat tgcagctctg
     2221 attattgagc cattagtcca atgcgctggg caaatggcca tgcactcggc catcttcgtc
     2281 cagaaagcgg cggcgctttg ccaacaatat ggcgtccatc tcattgccga tgagatcgca
     2341 gtggggtgcg ggcgtacggg aacattcttt gcctgtgaac aggctggtat ttggccagac
     2401 ttcttaacct tatcaaaagg cattagtgct ggatatctac ccttatccat ttgtttaacc
     2461 cgcgactcta tttatgcagc cttttatgcc gatgatttaa atcaagcatt tctgcattcg
     2521 cattcttata ccggcaaccc ccttgcttgt cgagcagcct tagcaggatt agatatcttc
     2581 aaaaatgaac aaatactttt aaagaataaa gaaagtacaa caaagatttg ggaagcattt
     2641 gcgtggacgc tcgctgatga acgaattaag cacgttcgtc agaaaggcat gatctttgca
     2701 tttgatattc atgagcactc aattaaaaac ccagaaaccc tctcaaaaga gttatttcaa
     2761 agggggatgc aagaaggtgt tttgataaga ccaattgggc aaacggtcta tgtcatgcca
     2821 ccttatattc tgagtgacac agaaatagaa ttaatgagta agggtatttg ccaagccctt
     2881 aataaaaccc ttaatacctc taaatagtcc taaagatatc tagcagatat gttctttgta
     2941 aaaaaccaaa ttactcaggg actagcagaa ctagatgctc agttactcag gcgcaagatc
     3001 cgaaccgttg aatccccatg tggcacacga atacacattg caggacgaga actacaagcc
     3061 ttttgcagca atgactacct cggctttgcc aaccaccctg aactaattaa tgcacttgca
     3121 gagggagcgc ggcgatttgg ggttggcagc ggcgcctcac atttaattag cgggcatcaa
     3181 acgattcatg atttgctaga aacaaaactg gctcagaccc aagctcaatc aatacctaag
     3241 gctaacgccc tgtttttttc taccggctat ctagccaata tcagtgcgat tactgcactc
     3301 agcaatctca agagtagtgc ttccagaatc tattcagctc gcctaaatca tgcctctcta
     3361 attgatggcg tccggctcgc caaagggcaa agtaatgccg atgtgtttat ctttgatcat
     3421 cgcgatctgg gtccgctcgg ggatgaactc aaacgagatt cttctgagca caaactcatc
     3481 gtcattgatg gtgtcttcag tatggatggc gatttagcac caataggcga actacttcaa
     3541 ctagctgagc agcatgatgc cttactttta atcgatgatg cccatggctt tggtgtttta
     3601 ggaaatcagg gtcacggcat tttggaagat agtggtcttt gctccgaacg tcttatttat
     3661 attggcactc ttggcaaagc agcaggcctc agtggagcgt tcgtttgtgc tcatgagctc
     3721 ctgattgagt ggttaattca aaaggctcgc ccctatattt acagtaccgc aagttctcct
     3781 gctgtagcct ttgccttaat acaaagtctt gatctgataa aaggtcgagc tggtcaggaa
     3841 gcccgtacca aactgcgaag caatatcacg cattggcaaa atctcgcaca atttaagacc
     3901 tggcaaatat taaagtcaga tactgcaatt cagcccattg tcgtcttaag caatgaaact
     3961 gcacttcaac tagccaaaga tttagacctt gccggatatt ggattccggc gattcgtccg
     4021 cctaccgtgc cacaaggaat gtctcgtttg cgaatgactc tgtctgccac ccacacccat
     4081 actgagattt ctgtattagc aaaaactctt ttcgatcttg aatcgaaggg atggtcatca
     4141 tgacacattc ttactttatt actggtaccg atacagaggt tggcaaaact ttagtaacgg
     4201 ctgctatcgt ctataaatta aatcaatcta agcatggctc tgcttcagcc tttaaacctc
     4261 tagttgcagg cacttacatg gaccaagtcg gtcaattacg taacgaagat attgagacct
     4321 atgtaattgc tggtggtaat aaaattccaa gagagttgct ctgcccatat gttcttgaat
     4381 ttccagctgc gccccatttg gtagcaaaag aaaagggtgt caaattagat ctccaagaaa
     4441 tgctcaaggg ttttcaagga attcaacaag cggtagatca tgtgattgtg gagggagcnn
     4501 cttattttgt ctgtaggtat tcgtttgggt tgcttaaatc acactttatt aacggtggaa
     4561 gccattgaaa aaagaggctt gaagttagcc ggctgggtcg ctaactgtat tagccatgaa
     4621 atgccatacc gcgatgaaaa catcaacgca cttcgtgacc gtattactgc acccttactg
     4681 ggcgtgattg agcccttacc aaaatccctg caaaaagcag tcaatgcgcc ttattcttta
     4741 gaggcaatca agtttgctgc cgattctata gtgctactaa acgaagaatt tgactaagta
     4801 ttactctcat cgcgcagaac acggcgcaag attttgccca cattattctt aggcaggctt
     4861 tctctaaact caatttgtgt tggccgtttg tagtgcgtaa gctctcgatt acaaacgtcc
     4921 atgatgagac tctcggtgat atttaaatct tcacgcacta caaataactt aactgcctct
     4981 ccagacgatg tctgtcgcac accaacaaca gcgcactcaa ccacgcccgg aatcgaagcg
     5041 acgatatctt ccacttcatt cggataaaca ttaaaaccgg ctactaagat catgtctttt
     5101 tttcgatcaa caatcttgaa ataaccggcc tcatccatta aaccaatatc acccgttcta
     5161 aagtagccat ccgatcccaa gacattggag gtttcgctct cttgttgcca atagcctgac
     5221 atcacttgtg gtccgcgaat ataaatttca ccaacctgac ctgctactaa atctctccca
     5281 tcctcatcac ggatacacat ctcggtatcg ggtagcggta agccaatcga gcccgtaaat
     5341 tcttgagcaa gtggcgtgtt acaagatgcc actggtgagg tttccgacaa accatacccc
     5401 tcagtaataa ccgttcctgt taatgcctgc caacgctccg ccacagatcg ttgtaccgcc
     5461 atacccccgc caatagttag ggcccagcgc gagaaatcta aatcggtaaa cttggagtta
     5521 ttgagcagcg cctgaaacaa ggtgttaacg cctgggaata catttatttc tggaaattct
     5581 ttcaaaattc caatcaagtt cggcaggtcg cgggcgttag gaaccaaaag tatccgtgct
     5641 ccaagacgca tacctaataa accacagccc gttaatgcaa agatgtgata catcggcaaa
     5701 gcacataaaa aatgccaggc ttctatagtc ttgcgttgct gaatgggttt tagccatgcc
     5761 tcaatttgca tgacatttgc aattaaattt cgatgggtca ggatcgcacc cttcgatata
     5821 cccgttgtac caccggtgta ctgcagtaga gctatatcgc tctgcgatat agctggtttt
     5881 tgatagctta gctgcgctcc acgctcaagc gcttgactaa attgaatact ctgtaagtga
     5941 ttgggtaata cgtatggaac taccttgcgc ttaaatgtac gaataaagcg atctaaccaa
     6001 gggcccttcc aacccaacat ctcggcgatg ctagtgatca ccacatgctt aagtgcaatc
     6061 gcttgccctt ctattgcaaa tgcatcctca agagttttac caaaatgctc cagcaataag
     6121 atagtctctg caccacaatc acttaattgt gcagataact ctcggggcgt atacaaagga
     6181 ttaatactga caacaatata tccagctcgc aataccgcat ataaagcaat tggatattgt
     6241 ggaatattgg gcagcatgat agctacccgc gcacctttct ttaaacctaa gctttgtaga
     6301 tatgtagcca gatttttaga ggcatgatca atggcttgat atgaaaaatg cttgcccatg
     6361 caccaatatg ctggctgatt ggcatgctta gtaaatgact catccaaaag atctaataaa
     6421 gagccatatg ctccgtaatc gagctcatgc ggcacgccag cagggtagga cgaacgccat
     6481 ggtttatggg taatcatcag tatttattgc actttttttc agttaaatcg ccttaatgct
     6541 tagtgtatcg ctttgggcta atattgttct atgagaatcc ttcctgaaat ccttcaatct
     6601 gcccaatcga ttgcacaaat ccgccgcaat atccatgctc atccggagtt gcgttttgag
     6661 gaaaaacgga ccgctgagtt agtggcagag gcactttcta gctggggaat cccaatattt
     6721 aaaggtttgg gtaaaactgg ggtggtcgga cggattgatg gcgagcttgg tcccggaaaa
     6781 atgattggtc tgcgagccga tatggatgcc ctgcccttac aagaaaaaaa tacctttccc
     6841 cacgcctcta cccatgctgg aaaaatgcat gcctgtggtc atgatgg
//
LOCUS       DDPQ01000012            3294 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_24257,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000012 DDPQ01000000
VERSION     DDPQ01000012.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_24257"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    899..947
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1518..1608
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2037..2096
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          825 a          731 c          768 g          770 t
ORIGIN      
        1 tagtctaata gattgcacgg tgatggggca gattttatgc gcccccaatc tcagcaaaaa
       61 ccctaaaaga actggcgagc ccgatctaaa tccgcctggg tatcaacgcc cgggggcggt
      121 gactctgggg tgatgtgaac ggctatggcg taaccatgcc acagtgcacg tagttgctct
      181 aaagattctg cctgctccag gggtgaaata gccaagttag agaatgcttt cagaaaacct
      241 gttctgtaag catagatccc caaatgccga tagtagatag gagagctgtg gttgcgatca
      301 aaagggatgg tagatctgga aaaataaagc gcctcattat ttttgctgag aaccaccttt
      361 acagcatttg gattggtaat ctcactctga tcatgaatag ctactgcagc agtagccatc
      421 gcacaatgag gcttggattg caaagtattg gcaacttgat taatgagatc gaccggaatg
      481 agaggctcat caccttgcac attcacaaca atggcctcat cgtttaagcc taattgcaga
      541 gcaacctcag caagccgatc agtaccactt gggtgatctt cacgggtcat catgacctct
      601 agattggcag ctttgcaaac ggactcgatt tcttgcgcgt cggttgcgac aatgattcgg
      661 ttggctgccg attgggccga acgttcggca acccttataa ccatgggctt gccccctaag
      721 tctgcaagtg ctttgcgctc gagccgagac gaagcaagtc tggctgggat aacaacagtg
      781 aatgagccca tcttctgttc ccctatttaa gaggagagtg caatcgcttc ttcgggggtc
      841 aaacttctag cctcatccac caacattact ggtatgtcat cacgaatggg gtaggctann
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg ctaggtagtt
      961 tgactggact caaatatcat catagcccat aagcctttaa atgactatcg ccgataggga
     1021 tcgggtcttt gaatcacttc ttgcacccaa ctgcttagtt cattggacaa aggtaaatgc
     1081 atggggacta cccagatacg gcgatcctcg atcgcagagc atttaactgc atccttttca
     1141 gttatgaaga tgaaatcagc atcaatctca gcaatgtccg tatttttaat ggccacatga
     1201 tcgggtaatg acaaggtatg ctcaagatgc attccaagat tacttaatgc gctaaagaat
     1261 ttctggggat taccgatggc cgcaatcgcc gcaatcttta agccgattag cttagtgagt
     1321 aattcggtaa atgattgaga ttcattggga cgatgcagtg gatacgcgct atcaatttta
     1381 ggtgttagct gaaaagactt ttctgcagca agggtatttt gtaattcgtc actagcgcca
     1441 atggagatgg tggcatcacg ctcacgtttg gcagactctc gtaatggccc agcaggcaaa
     1501 agtcgaccat taccaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ccaacgcacc
     1621 aatccagcat gttgtaagcc atcatcacta ataatgacgt tgacctcggg gtgagctttg
     1681 agtagagcat taatggactg cgcacgctta ggaaagaccc aaatcggtat ttgatggtga
     1741 gtgcgctgcg ccatgagaac gggttcatcg ccgacggtat ttggattact agtttgtacg
     1801 acctcttgcg gagcttccag ggtactttgc ccattgggtt gatagccacg gctgataata
     1861 cctggcttgt atcccgcttc gaataaggcg tttgcgagag cgattacaag aggggtttta
     1921 cccgtacccc ctacccgaat attgcccaca ataatcaggg gtaccggcgc agcacgatgc
     1981 attccaagag tctgagtaat gcctgtatca atcaagatct gcttgagcca aacaatnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttt
     2101 cccaaaagcg cggcgcagat ttgaagagca ttatttcttg gcttgactac taaaggcaac
     2161 gttgggaaga cccgcgtcac gcgcaagctc aagcactgtc atcacagctt ggtgaggtgc
     2221 gcgcgcatcg gcatcgatac tgatccgcag gttttgctca ttagcaccag agcccatttg
     2281 tttgagagcg ccagcaagct gtgagcggct cataactctg ccattgaggg caaagcgccc
     2341 atcgctactg actgccacat taatctcacg cgcagactct acggtatcag cgccagatgc
     2401 gctaggcaga gtaatcgcaa tctcctggaa ctttgtaaag gtggttgaga tcattaagaa
     2461 gatcaagatc actaacaaga catcaataaa tggaatgaga ttgatttcgg gttcagcata
     2521 ggagaaggag cgtgccgagc gatttagccg accgccgaaa gcgcgagcat tttctgaaga
     2581 acggttctgt agccaggaca caggattaat gactatttgg gtacaacttt ttaaagagct
     2641 gacgtgtaca ttcttcgcat tcacgttggc gttgatcggc tactgctcgt aatactctcc
     2701 aaccggctag cgcaggaatg gcaattaata aaccaaatgc ggtgttgtaa agtgcaatcg
     2761 agatgccgtg ggctaattgc tgtggattgg ttgcgccgac actcgcggca ttgacactgc
     2821 cccccataga tcccccttgg ctgccaaaga tctcaatcat gccaatcacc gtaccaaaga
     2881 ggccgagaag gggtgctacg gtcgcaatcg ttgctaaccc ccctagatag cgatccaact
     2941 tagcccacag attttgagcc aggatttgga gttcttcaat agctgcttcg gctgagtgcc
     3001 ccaaggcttg ctcgcgcaat acacctgcaa gcaggacgcc aatgggcgag gaggcactga
     3061 gatcgctcaa gaactcgcta ttggcgacat ccctagcatt tgcattaaat gcctgctcaa
     3121 tcgtttcttt acggatgatg tgtttctttc gcaaatacca agcacgttca ataaggatgg
     3181 ctaagccaag tatcgagatt gcaagaagag gccaaatcgg ccagccggca gcaagtaaga
     3241 tggagtacat aggtggctat tttcaatgaa aacgatttct gcgttcaaaa taat
//
LOCUS       DDPQ01000013            2801 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2907,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000013 DDPQ01000000
VERSION     DDPQ01000013.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2907"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    141..162
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          707 a          614 c          768 g          690 t
ORIGIN      
        1 aaagcagata ttgaccccgg cgccgcaaca cgtgctcaag cctttattac aagctcactg
       61 cgtttaatgg gatttcaggg gaaccccgat catgccaccc atgtgaagga ggagtggcac
      121 cccacagtct tgcgcatgag nnnnnnnnnn nnnnnnnnnn nngaagtaaa gcgttttcag
      181 aaactcgaga ccgccaatgg tcatctgcaa gagaggcgta agcaacaagc cagctcttgg
      241 atgtgggagc ggatcgatag tgctctcaaa agccagttca agaaccatcc agccgtccaa
      301 cacttactgc ccgagatgag cgttgcggtt aatcaaggaa gcatagcccc atcggttgct
      361 gcccgtcgac tactcgaagc gatgaagttg taaggctacc caatttatta caggagtttg
      421 tatgcaagac attatttctc aactagaagc caagcgcgag ttagctcgcc ttggtggtgg
      481 tcagaagcgg attgctgcgc agcatgccaa aggcaagtta actgctcgtg agcgcattga
      541 gctcttgctc gatgaagact cttttgaaga atgggatatg tttgttgagc atcgctgcac
      601 cgattttggg atggccgatc aaactgttcc gagtgatggg gtagtaactg gttatggaat
      661 gattaatggc cgcttggtct ttgtgttttc acaagacttc acggtcttgg gtggctcgct
      721 ctccgaagcg catgccgaga agatttgtaa gatcatggat caggccatga aggtgggtgc
      781 acccgtgatt gggttaaatg actctggtgg tgcgcggatt caggaaggag ttgcatcgct
      841 cggagggtat gccgagatct ttcagcgcaa tgtctctgcc tcaggagtga tccctcaaat
      901 atcgctgatc atgggaccct gtgcgggcgg tgcggtgtat tcaccggctt taacggattt
      961 catcttcatg gtgaaagaca cttcttatat gtttgtgacc ggtcctgaag tggttaagac
     1021 cgttacgcat gaggatgtca gtgccgaaga tttgggaggg gcaagcacgc attcatcggt
     1081 ttcaggagtg tgtgacctag ccttcaataa tgatgtggaa gcgatcatga tgttgcgtcg
     1141 tttctatagt tatctgccac tgtctaataa agagaaagca ccattcttgg gcggcttggt
     1201 ctggtcagat gaacccgatt attccttaga taccttagta ccgaataatc ccaatcaacc
     1261 ctatgacatg aaagagctga ttcataaggt ggttgatgat gaggacttct ttgagttaca
     1321 gccggactat gccaagaaca tcatcatcgg ctttgcacgc attggtggac aaactgctgg
     1381 cattgttgca aatcaaccct tagtcttggc cggttgtttg gatatcaaat catccatcaa
     1441 ggcagcgcgc tttgtccggt tctgtgatgc cttcaatatt ccagtagtaa ccttggttga
     1501 tgtgccaggc tttatgccag gaaccgcaca agagtacggc ggcattatta agcacggtgc
     1561 taaattgctc tatgcctatg ccgattgcac tgtacctaag gtaacggtga ttacacgtaa
     1621 ggcctatggt ggtgcctacg acgtgatggc ttctaagcat ttgcgtggcg atgtcaactt
     1681 tgcctggcct tctgcagaga ttgcggtgat gggacccaaa ggtgcggtgg agattatttt
     1741 ccgtgaggag aagggcgacc ccagcaagat tagtgagcgc gagaccgagt acaaagcaaa
     1801 atttgccaat ccctttgtgg caggacgtcg cggttatatc gacgatgtga tcatgccgca
     1861 cgagacccgc aagcgcattg cacgctctct agcgatgttg caagacaaag acctacaaaa
     1921 cccaccccgt aagcacggca acattcctct ttaattgaat gaccagagca tattatgttt
     1981 aagaaaatac taattgcgaa tcgaggcgag atcgcctgcc gagtaatgct tacagcgaaa
     2041 cggatgggca ttcagaccgt tgctgtgtat tcagaggcag atgcggaggc gcgccatgtg
     2101 cgcattgccg atgaagcagt gtgcattgga cctgcgccat cgcgagagtc gtatttgcag
     2161 atggatcgca ttattgcagc ctgtaaagaa acaggtgcac aagccgttca tccaggctat
     2221 ggattcttat ccgagaacga agagtttgct agacgagttg aagaagaagg cattatcttc
     2281 attggcccca agcatcaatc gattgctgcg atgggcgata agatcgcttc taagaagctg
     2341 gctctagaag ctaaggtcaa cactatccca ggtcacaacg cagcgattga tcaacctgag
     2401 gatgcggtaa agatcgccaa agggattggg tatccagtga tgattaaagc gtcagcgggc
     2461 ggtggaggca agggcttgcg cgttgcctat aacgatcaag aaactgccga aggatttgtg
     2521 gcctgcaaga acgaagcgcg taatagcttt ggtgacgatc gtgtcttcat tgagaaattt
     2581 gttgaagagc cacggcatat tgagatacag gtgctcggtg atgcacatgg taataccgtg
     2641 tatttgtggg aacgcgagtg ctcgattcag cgtcgtcatc aaaaggtaat cgaagaagcg
     2701 ccatccccat ttctggatga tgtgacccgt aaagcaatgg gagagcaggc ggttgcatta
     2761 gcgaaggctg tgcactatca atcggcagga actgttgagt t
//
LOCUS       DDPQ01000014            5934 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_25218,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000014 DDPQ01000000
VERSION     DDPQ01000014.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_25218"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT         1644 a         1337 c         1469 g         1484 t
ORIGIN      
        1 ctttcaagaa ctgctctaaa cgtagaaacc tcaatgatct cgggacgctc tttaccaaaa
       61 aaagtaacgt gtacgatttg aaagcgacgc ccaattaaac gggagcgccg aaataatttt
      121 tgtacttgtt cgggggttgc attggtagcc acatcaaaat cttttggtgc aattcctaaa
      181 acgaggtcac gaaccgcgcc gccaacaatg taggctttgt aaccagcctg ctgaagagtg
      241 tgggttactt tgaccgcatt cttggataaa agcccaggat taatccgatg ggtctttttg
      301 tggactttat gagcaacaag ggtggcccca gattgattgg atttagggcg cggacctcga
      361 cgcaaaagac gatcgaggaa cttagtaatc atgaagggta tttagattat tggatgaaga
      421 gaaaagctcc aaaattggcc aatgacgttt ttctgcttca gcccgtaaac gcccatccgg
      481 attggtagca acgggattgc tgacgcattc taataaggga agatcgttca tggagtctga
      541 ataaaagtaa ctcttctcga ggctgtcaag cgataaaccc tgctcttgaa gccaggcgtt
      601 tactcgtgtt accttacctt ccttaaagct cggcagccca gcgaccttgc ccgtaaagcg
      661 tccatgggct tgattatttt cagtggcagg atcagtcgca accaaatgat caatgccaaa
      721 ggctttcaca atggggcgcg tgacaaagct atttgtggcc gtcaccacac aacataagtc
      781 accaactgct tgatgttttt tgaccaaggc caatgcattg ggatgtagct gcccattaat
      841 tttctcgcgc ataaaggcat catgccactg ttgtagttgt tcccgtgtat tttcagacaa
      901 aggtttgaga gcaaagcgta agaacgcatc aatgtctaat ttaccaactt tataatcagc
      961 ataaaactgc tcgtttgatt tagcgtattg ctcaccatca acaactccct ggcgaactaa
     1021 aaattgcccc cactcgtaat cactatcaaa aggtaagagt gtgtgatcga gatcaaataa
     1081 tgccaaacga ctcatgattt agtccctgca atttgaagaa gttcacgaac caatggcaac
     1141 gtaattgctc gtttggtttc aagcgagtga gcatcaagag catcgagtaa tgccatcaaa
     1201 ctaggcatat ctcggtaaaa atgactgata agccagggta gaacgtcaac cgacaaattt
     1261 aagccccgct ccttagcggc atgggtgagt gcagacactt tttcagagtc ggtcagtggg
     1321 tgaatctgaa aaataagtcc ccaagcaaga cgggtacgaa gatcttcacg aagctctaaa
     1381 ccgttgggcg ccatacttcc agctaggtaa acaaagtagt tagagtttgc atgaaccaag
     1441 ttgagtattc gaaaaagact tgcctgtagc cgatcgtcaa gctgatcaac atcgtccacc
     1501 gtcatcacaa ttacctcttt gctataggag cgagataatt gatcctcgca ctcaatccaa
     1561 gcattaggtt gattggggtt aagttcgata aaggctatag cgcgttcctt ggcagcatat
     1621 gacattgcct taagaagatg agttcgacca caaccgttgc ttccccacca atagagccaa
     1681 cgctcctcaa tcagcacttc tctttctgag gcagatggcc aagcagcaat agatcgctca
     1741 agtgtatttt tcagctcttc attgccgctg actaagaaat tatgaagtga agcagggggc
     1801 gtttggttaa ggtccagtgc aaattgacgt ggtagggcgg atgaatgatt catgaacact
     1861 tcgttatttg cgataccacg cgctttgttc gtattgcgac catacaaagc gaatcaagac
     1921 cagcacgatt gcgctggcag gtaatgccag caggacccca aagaagccaa acaatttagc
     1981 gaagatcaat aaggcaaata gaacggcgac cggatgcaaa ccaatgcgtt cgcccacaag
     2041 gcgcggggtt aggaaaaacc cttccagcac ctgtccaata ccaaaaatca ccaagactac
     2101 gattagctca gcaccaaagc caaattgcag aatggcagaa aggatagcca gaaccaggcc
     2161 aattgcaatg ccgatatatg gaataacaat aaccaaggcg gtaaagaccc caagaccaag
     2221 cgctccttcc ataccggtga gactaagacc ggcgctgtag aaaaccgcca tgatcaaaat
     2281 gaccaacatc tgtccgcgta agtattggga gagcagccca tcggtttcgc tgacgaggat
     2341 ttgtgcggtt acttgaaagc gaattggaat caattgtttg agtaaagcaa aaaagtgatc
     2401 ccaatcgatt agtaagtaaa agagtacaaa gagaattagg atgaagttga caaacccgcc
     2461 caatacagaa ctaccggagg ccaaaacagt ttccatgctg gtactcaaaa tctgattggc
     2521 atttgcagaa aaatgcgcag tgatcttttg ggtaatggct tctcgtaggg cttgccaatc
     2581 aatatctata ttgaggctta atagttttgg cgcgagccat gtttggatac tttgtaacca
     2641 aataggtagc tgagctttaa tcagggggat ttcatggcgt agcagattga ttagtaatag
     2701 aaggatgacc gccagtacgc ttaggccaaa aagaatgctg attgtgctag caagggcttt
     2761 acccaggcct attgtctcca agcgaagaca gaccggccgc aggatgtaag ccaagataaa
     2821 agcgaagagg aagggggtaa aaatttctgc catacggcct catactctag aattcagaga
     2881 ttctaccgat caaattgaac gaataagcgg atttccctga tttcccaaag attaaacgat
     2941 gaacaattcc tctaagcccc cctcatccca aggactctcc taccgtgacg ccggggtcga
     3001 cattgacgcc ggcgacgcct tggtagaccg tattaagcct ttggccaaga aaaccatgcg
     3061 agatggcgtg ttggccggaa taggcggctt tggggctctt tttgaggtcc ctaagcgata
     3121 tcaggagccc gttctagtgt caggcacgga tggcgtcgga acgaagctta aattagcctt
     3181 tgagtggaac tgtcatgaga ccattggcca agatttagtc gccatgagtg tgaatgatat
     3241 tttggttcag ggggcagagt ctctattttt cctcgattat tttgcttgcg gcaagctgtc
     3301 agtcgataca gcgacaaccg tcgtggccgg cattgccaag ggctgtgagt tagcggggtg
     3361 cgcattgatc ggtggcgaga ccgctgagat gccaggtatg tatccgccgg gtgaatatga
     3421 tttagcgggc ttcgcagtcg gcgtagtcga gaaatctaaa attattaatg gcgcttcgat
     3481 taaaccgggt gatgcagtaa tcggtattgc gtccagcggg gcgcactcca atggctattc
     3541 tttgatccgt aagattatcg agcgagctgg cgcaaagcca tcagaagaga tcggcggacg
     3601 ttcattgcgt gaggtggtca tggcacctac ccaaatctac gtcaagcaat tgttaagtgt
     3661 gatggcccaa catcccatta agggtttggc acatattacg ggaggtgggt tggttgataa
     3721 cgtgcctcgc gttctacccg agaatacgca agcgatcctt cgtcgagatt cttggaagat
     3781 gccagatttg ttccgctggt tgcaaatgaa gggcggagtc gccgacgcag agatggtacg
     3841 ggtatttaat tgcggaattg gcatggttgt agtggtgagc catcaagatg ccgatgcagt
     3901 cattgctagc attaatcaac aggggcttca ttcctggagt ttgggtgaga ttatttcgcg
     3961 tccgcctggg gcgccccaaa cgatcgtgat ctaagttact gcaagagggc tagacattgt
     4021 ttttgggcaa gatccatttg ctttgtatcg aaaggatcaa atacccgacg atccattgct
     4081 cgaagccagg tcatctgccg tttactgagt tgtcgggtcg cagcatatgc tttttctttc
     4141 atcgtctcaa aatcaatatc ccccgctaga tactcccaaa cttgtcgata accgacagac
     4201 cgaatcgctg gcagattggt atgtaggtcg ccacgattgc gaagttgttc aacctcttca
     4261 atgaatttgc tctctagcat ttgatcaaaa cgttgctcta gacgcgtgtg caaagtcttt
     4321 cgatcggatg gctcgagtga gattacctct acccaatcag gagtgggctc ttgcttccga
     4381 cccgccagac tcggggtttc ttcaaagaga tccgatagcg gtcgcccagt aagtgcaaat
     4441 acctctaggg ctcgctgaac ccgttgactg tcgtttggct gaagacgttc tgcactagcg
     4501 gggtccaaga tggctaactt tgcatgcata gcaggccagc cgatttcagc agcttcgcgg
     4561 tctaattgag cgcgcacctc agggtttgca gcgggtagat tagaaaagcc atagacccaa
     4621 gctctccagt agagcatcgt gccaccgact atgagcggga tatttcctcg ctgtttgatg
     4681 tcagaaatca gttcttttgc atcgcttgca aattgcgcag cagagtaatt ttggctgggg
     4741 tctaaaacat caatcagatg atggaccacc tctgcctgtt ctgctttgga gggtttggcg
     4801 gtgcctatat ccataccttt gtacaccagg gcagagtcca tgctgatgat ttcaataact
     4861 cgtcccaagt ctttatattg acgcgcaagc caaagagcta aattactttt gccgactccc
     4921 gtggggccga cgatggataa aactttagta ggcatcaaga acaaaagact tagtgttggc
     4981 gaacttgatt ttcaagggtc gaaatgtgct ctcggatttc ttcagcatca ctggcatccg
     5041 gttgcccgct gagataggtt tgcatatctt caattgccgg gcgcaagtat tctagttgag
     5101 caaaaattaa gcctcgatcg cgtacttctt cgagagagtc tggcaataaa ataactaggc
     5161 gttgttgtac tcccagtaaa cgctcccatc gttcatgttc ggtatatata agttttaagt
     5221 tacgcaagaa gcgcgaaagg atttctcgag aactagagac cctcagaaaa acatttagtg
     5281 gtagctgcag atcgccaaca tagccccttg cttctaagta tgggtctagc atttcttgta
     5341 actcatgctt tgccagagag gtgcccgtca aggggtccat gatgacctca ccctgaggga
     5401 gtgagacgcg caacataaag tggtttggaa aggatatccc tttaactttt aaaccaattt
     5461 gattgccaag ctccatcatc aggatggcta gagaaatggg aataccacgg cgactttgca
     5521 ggacgtgatg gatatacgag ttctcggggg cataaaaatc attggggttg gggccaaaac
     5581 caagctcggt ataaaagaaa tgctttagct gttgcaagcg ttgcacagca gaggcatcgg
     5641 cagaaatacg tttctttaga cgatcaccaa actgatcgat ttcatctaag acgtgttgaa
     5701 catctagatc agggaaggca tgttgtgcaa ccgcaatact tgcttcagta agcggaaagt
     5761 gctcgtcctc agcaactaag gtagagaagt aatcgagctg ttgagtcggc atatttgcta
     5821 ttttgcatga cgcagaaaat tttgccagcg caatccaacg agccagagag tggcaaagta
     5881 gagcaaggct gcaaacatca accaaagccc cagaaacgct atacggatcc atgg
//
LOCUS       DDPQ01000015           10147 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_8341,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000015 DDPQ01000000
VERSION     DDPQ01000015.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10147)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10147
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_8341"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1215..1283
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2265..2398
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3160..3235
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4453..4467
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7510..7540
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8824..8887
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9157..9208
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2623 a         2341 c         2282 g         2456 t
ORIGIN      
        1 gatattgcca tgaccggcga gatcaccttg cgtggcgaag tattgccaat tggtggcctc
       61 aaagagaaat tacttgcagc tcatcgcggt ggtatcaaat tggtcttaat ccctgaagaa
      121 aacgttaagg atttgatcga tattccggat aatgtgaaga acgcgatcga gatcgtgcct
      181 gttcgttgga tcgacaaggt cttagagcta gcgctagagc gcaagcccga ggccctgcca
      241 gaccctagcc cagaagagct ggccaagaag gcnntgtttt ttggggtgct tagctcagct
      301 ggtagagcgt ctgccttaca cgcagaatgt cagcggttcg atcccgttag cacccaccat
      361 tccttttaat ccatgctcga ttccgtccgc aaacaccaaa aaatactgca gatcattctg
      421 cttttattca ttgttccctc gtttgcgttt tttgggatct cgagctactc cgacttcttt
      481 gacaaagata ctgacctcat taaggtcaat ggcaaagcaa ttacctccct tgaggtagac
      541 aacaatgcca agcgtcaagc agagcgcttt gggggaaata gccaaattgc acaaagcctg
      601 ccatttcggc aagcggtcct cgatgagctt ctccaacaac gtctcttagc atttgcagtg
      661 aatgatctca agctcaatgt gagcaatgca tttctcagcc agaccttggc acaaattcca
      721 gagatcaaag cgctctataa acctgacggc acatttgatg cagcaaagta tcgacagttg
      781 cttgctaacg ttggtatgag tgttgatcag tttgagaact ctcaacgttt tgacatcatg
      841 gtgcaacagt tggttacctc agtagcacgt accgagttac ccaataaaaa gctgagcgac
      901 attatctcga ccatgtatga gaccgagcgc caagtacaag cattgcgctt tactgcatcc
      961 gactatgtaa acaaggtatc gcccaccgat gctcagttac aagagtatta ccaagccaat
     1021 accaaattat ttgaagctcc tgagaccatc gatgttgagt ttctggttct aaaggcagac
     1081 cctaaagagg atgctaaggc ctttaatgag aaggccgatc agtttgccaa catcacctac
     1141 gatcaagccg atagtcttaa acctgcagca gataagttaa agcttagtat tcaaagtgca
     1201 aagggagtgg ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnntttaatg aagaggctct taagaacaaa cgtaatatcg
     1321 aggcagtaca gatagcgccc ggtatctttg tatctgcacg ggtaaataac ttctatccag
     1381 cgaaggtctt agcctttaaa gatgtgagcg cagaagtaaa acggcagttc agcatcaagg
     1441 ctgctcaaaa gattgcggct gaagcagcgg gcacacgctt tgaggctttg caaaagaacc
     1501 ccaatgacac caatggcttt gctaaagcag tctgggtgtc acgtaataag ccgactgatc
     1561 tcttggcacc acaactcgat gccatcatgg cagttgatcc tgccaagtta ccagcggtgg
     1621 tctcctccac gaactccgat ggcggcatca ccctctatcg cgttgaccaa gtacgccaac
     1681 cacctgccag cgatgccaaa gtacgcgcag cacaagcaca acagattgaa gcattagcag
     1741 cgcaagctga gtttgcagcc ttccttaatc acatgcgtat tctggcaaac gtgaaggtga
     1801 ttaatcctct caagccagca ggtacatcca ataattgagt agtcgaacaa ggatgtcatt
     1861 aaatcaagat ttatccgagg attctagttt tgatgaggaa ttttccgcaa agctagaaag
     1921 agccattaaa gataatccag atttaccacc ggaatttgtt aaagatattt tgctgagtct
     1981 aaaagaaact gaattcattc agataaatcc ctttagttct tcagcatctc cctaaaaggc
     2041 tccatcgcct tccatacatt gccaaacata atgggctgcg cttgctcatt tggatgaatc
     2101 cgatcggctt gaaagagttc tggtctagta gctactccct caaggaagaa gggcagaaga
     2161 gctacttgtt cggattgagc cagactcaca aataaacgtg caaattgctt ggtgtattcc
     2221 tgtccgtagt taggagggat ctgcataccc agtaagagaa cctcnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     2401 gtttgtgatc tgccacgagc taccttcttt gcgtaactga ttacccaaca aggttaccca
     2461 gccagcccca cggggtagac catattcagc agaaagacta tcgcccaata ccaagagttg
     2521 tggaccagac tgtggggctt taatgctctg tgccagcaaa ggtgaactgg tcacgagtaa
     2581 gatggaggcc aatccaacga caaattgttt aaacaaaggt ttcacattga gtattgtgca
     2641 tgaatctacc ccagtcttgc aggccagtca tgtgggtaaa acagtccaga ccagtgatgg
     2701 acccctcgca atcttgcagg agattgattt ctcgatcaca gcaggcgagt ccatcgccat
     2761 tgtgggtgca tccggttctg gtaaaagcac tctcttaggt atcttggccg gcttagatct
     2821 cccaagtagt ggcactgtgc acatgatgca agaggatcta agcaagcttg atgaagatgg
     2881 ccgtgcccga ttacggggtc aatacgtcgg cttcgtgttt cagtccttcc agcttgtgcc
     2941 gcatctcaat gctctggaga acgtgatgct gcccttagag attcggggtg atccacttgc
     3001 aaagtcgcgc attgccgccg cagcctgctt agctaaagtt ggtcttagtg agcgtctctc
     3061 gcattaccct aaaactctat cgggtggaga gcagcaacgt gttgccctag cccgcgcctt
     3121 tgtgagtgag cccaatatct tgtttgccga tgagcccacn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcaa
     3241 tcgtgagaac ggctccaccc tgatcttggt gacccacgac ccacaactgg ctaaacgctg
     3301 tagccgccag ctcacccttc agggtgggcg tttggtctaa accaggaagc agaacccacg
     3361 gcactcttat aatcacggaa tgtcattttt ccgttgtctg cccggggccg atgccctttc
     3421 tgtcttccgt caacaacggc tcttaagcca cctttcaact caaggaattg agcttagtag
     3481 catcactgca cagtacctgc attttgtctg gtcagatcaa gagctctcag ccagccaagt
     3541 cgccacccta gaagctttat tgcattacgg cgagcccttt gcaggaagca aggcaaacca
     3601 atccgcaatc gtgattcccc gatttggtac ggtctcccct tgggccagta aggccaccga
     3661 cattgctcgt caatgcggtt tagaggtctt acggattgag cgcggtgtgc gttaccaatg
     3721 gagcgccaag agaaatctga acgcctcaca agagcagatc ttggagacag cattatttga
     3781 tcggatgacc gaagtcatcg tcactgctga gaacgatgta caagctctat accaaacact
     3841 acccgatcga ccattcacac gcattgatgt catggggcag ggtaaagctg cgctcgagca
     3901 cgctaatcaa tccttaggct tagcactctc tgctgatgag attggctatt tagaagagaa
     3961 cttcaagcgc ctagagcgca acccaagcga cgttgagctc atcatgtttg cacaagctaa
     4021 tagcgagcac tgccgtcata aaatctttaa tgccagttgg accatcgacg gtaagaatca
     4081 agagcactca ctgtttgcca tgatccgtaa taccgatcgc ttgcaaccga agggaaccat
     4141 cgtcgcttac tcggataact ccgccatcat ggaaggctgt gatgccgatg tatggagctc
     4201 agtaggccct gcgggacact atcaaaccaa gcgtgcattg gtacacacct taatgaaggt
     4261 tgagacccat aaccatccca ctgcgattgc accgtttcag ggggcagcga ccggatcggg
     4321 tggtgagatc cgagatgaag gcgcaacggg tattggcggt aaacccaaag caggcctcac
     4381 cggattttcc gtatcgaact tgcatattcc tggcacgaac ctcccctggg agaaaagtcc
     4441 ttatggcaag ccnnnnnnnn nnnnnnngca tttaataatg agtttggaag accaaacttg
     4501 ggtggttact tccgagtctt tgagcaaacc attggcggac aacgacgtgg ttatcacaag
     4561 cccatcatga tcgccggtgg tattggtgcg attgagtcta ttcatacgca taagcatccc
     4621 atcgaagatg gtgatctctt aattcagtta ggcggccccg gaatgcgcat tggcatgggt
     4681 ggtggtaccg ccagctctat gtcgaccgga gccaataccg cagatctgga ttttgattct
     4741 gtacaacgtg gtaatccaga gattgagcgt cgtgcgcaag aggtgattaa tcaatgccgt
     4801 gcactaggag aacataatcc catcgtctcg attcatgatg taggggcagg gggcttatcc
     4861 aatgcatttc cagagctagc cgatagtgca aaactgggtg cacaatttga gctacgcgct
     4921 attcctctcg aagagagtgg catgagcccc gcagagatct ggtgcaacga gtcgcaagag
     4981 cgctatgtga ttgcgattca tccgaaagac ctagatcgtt ttgccaagat gtgtgagcgc
     5041 gagcgctgtc cgtatgcggt cgtcggacac accactcaaa accgtcaact gcaattgcac
     5101 gatcaacagc aaaccaaaaa tccagatcaa cagtacccca ttgatatgcc gatggaagta
     5161 ctcttgggca aaccccccaa aatgcatcgg gatgttcaca ccacacaaga acagtttccc
     5221 ccatttaatt gcaatgcaat cgaactcaaa gaagcaatcg agttggtttt gcaacaacct
     5281 actgttgcca gtaaatcatt tctgattacc atcggcgatc gtacggtggg tggtcttaac
     5341 tcacgcgacc cctttgtggg cccatggcaa gtacctgttg cagattgtgc ggtgaccacc
     5401 atggattact ctgggtatcg gggtgaagcc atgagcatgg gtgagcgtac tcccgttgca
     5461 ctctttaatg caccagcagc agccaagatg gcagtgggtg aggcgattac taatattctg
     5521 gccgctgata ttgctagcat cgaagacatt aagttatcgg ctaattggat ggcagcttgt
     5581 ggtcaacccg gcgaagacgc caaactctat gagtctgtta aagcagtagg aatagagctc
     5641 tgcccagagc tgggtatctc gatccctgta gggaaagatt ctttatcgat ggctacgagt
     5701 tggcaggatc acaatcaagc aaagcaagtg gtttcgcccg tatcactcat catctcggca
     5761 tttgctcctg tgcatgatgt gcgcaagacc ctcacaccac aactgcaact ccagaccgag
     5821 accgaactaa tcctgattga tctaggacgt aatcaaaatc ggatggctgg aagtattgtg
     5881 acccaggtat ttgagcaaac ggatacacac gcaccgaata tcgatcacgc gagtgatctc
     5941 aaagccttta ctaaagccat cattgccttg cgtgagcgca atgctatctt ggcctaccac
     6001 gatcgctccg atggtggctt attagcctgt atcgccgaga tggcctttgc aagccattgt
     6061 ggtatctctc taaacgttga cctcttgtgt attgatccac agaccgaacc agattatggt
     6121 gatgccaaga actgggcttc tcaggtcagc ggtcgtcgtc acgagcaaac catccgcgcg
     6181 ttgttcagtg aagagctagg tgcagtcatc caaattgaac gatcgcagcg cgatgcagta
     6241 tttgcagtct tacgtgaatg tggcattagc gcctttagtc atgtgattgg caaacctaat
     6301 caaagcgatg ccttagagat ctggcgtgat gccaagtgcg tttacaccga aagcagagtg
     6361 catcttcaaa aactctggca gaacaccagt tatcagatcg ctcgcttgcg tgataacccc
     6421 aactgtgtag atagcgagta tcaaagcgta gaggatgtaa gtgatcctgg gatgagcccc
     6481 atcctgcgtt ttgatcctaa tgagaacccc agcgcaccat ttattaacaa gggcacacga
     6541 cccaagatgg ctatcttgcg agagcagggt gttaactccc atcttgagat ggcctacgcg
     6601 atggatctcg ctggctttgc tagtcatgat gttcatatga gcgatctact ttctggtcaa
     6661 gccaaacttg aggactatca gggcttagta gcctgcggtg gatttagcta tggcgatgtg
     6721 ctcggtgctg gtgagggctg ggccaagacc attctgttca acccacaact cagcgagcaa
     6781 tttgcaagct tcttccaacg taacgatagt tttgccttag gtgtttgtaa cggctgccag
     6841 atgatgagta atctagcgag cctaatacca ggtgctaagg catggcctaa gttcacacgc
     6901 aatcaatcgg agcaatacga ggcacggctt gtgatggtag agatacttca gtcaccatcc
     6961 ctgttcttta atggaatgag cggtagccat ttacccatag cggtcgcaca tggtgaaggc
     7021 tacgcaaact tcaaacacca aggcaacgca cgtgagattg aacataatca attagccgcc
     7081 atgcgcttta ttgatcatca gggcaatgcc accgagcgtt atccattaaa tccgaacggc
     7141 tcaccgcagg gattaactag cgtcaccaca gccgatggcc ggttcacagt gatgatgccg
     7201 catccagagc gcgtctttag gaatgtgcaa atgagttggt atcccaaaga atggaaccaa
     7261 actaaggatg gtgccagccc atggttacgc atgttccgca acgcccgcgt ttgggtgaac
     7321 taagtggcgc aaacacgatt acccgctgag ccggtcacgt tctcagagtc cggcgggatt
     7381 cggtatttgc atttaggaac accatggata caaggagcca tgcgtattcg ggatcctagc
     7441 gagatctatc tcgagtactc gcaacagatg atggcctggt tactcttttt acagagtaag
     7501 cccaagatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcgtaatca ataccgcagt
     7561 agagctgaac ccagcggtca tcattgcagc cagaaccatg tttgatctgc ctcatgatca
     7621 tgaccaatta aaggtagtgc aagacgatgc cctgagtttt gtgaagaata aacgccatca
     7681 ccagcagttc gatgtcttgc aagtagattt gtatgatgcg gatgccaaag gacctgtact
     7741 aagctcgctc gagttttata aggcctgttt taatacccta aaggccactg gggtgatgac
     7801 cgttaatttg ttctcacagc acaaaagttt tgatatcaac ctcaataata tgtgcaaagc
     7861 ctttcagggc agggtgctga tattcccgga atcgcacgat tgcaatgcag ttgccatcgc
     7921 ctttaagggc cccatgatca atgccaattg gaagaatatt gagcaacgcg ccaaaataat
     7981 ccatgatcag actggtctac cgactaagtc ctgggctaag ggcttacggt cagttaatgc
     8041 tcgccaggaa gagcgcttac aaatttaatg aagatacaga tgaactttct ggggagccca
     8101 atcgctagga aagttaaaaa ctatctgttc atcctgatta gcctctttat ttgctttgcg
     8161 caaatatcnn tatatcttgg ctgagattat tgcgcagacc ctaaaaaata atacggatta
     8221 cgagattaaa actaaattag ggcttggtaa tacaaccatt ttgtttagtg ccctcttaca
     8281 aggcaatatt gatgtctacc cagaatacct tggcacgatt accaaggaga ttttaaaaga
     8341 ggacggcacc ctctctttgg agcagatcaa caaaagatta cttccactca aaatacaagc
     8401 gggtatattt ctaggctttc aaaactccta tgccctagca atgagaaaag atcaggtaat
     8461 gcagcttggg atcacaacaa ttgcggatct tgcaaaacat ccaagcctaa agattggttt
     8521 ttcacatgag tttctgggta gacctgacgg atggccttcc attcagaaaa tttatggtct
     8581 ttcaaacctg agcgctaagg gactggatca tggccttagt tattccgctc ttgataagaa
     8641 acaggttgat ttaatcgatg catacaccac cgatgcgcaa ctcagtaatg aaaagattat
     8701 cctcctcata gataaccaaa gatttttccc ctcgtatgaa gccgttttgc tttaccgcat
     8761 agagagtttt aaagataaac ctaaagcctt attagcctta gagggtttat cgcagaagat
     8821 ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnngag caaatatctt ctgagcctct ttcatttatg aagcaattat ttgggccaga
     8941 ctttttgcgt ctaagttatg aacactctat attggtcttt ttgtcattgt ttattgccat
     9001 gatcattggc atcccgctgg ggattttgac cttctgcaat ccctcagtag gtaatccaat
     9061 gttgtatgta tccggaattt tccaaacgat cccatctctt gcattactgg cattcctgat
     9121 cacaattttt caaacgattg gaccgctacc agcaatnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct aacagttaat ccatcattac gtgagtctgc
     9241 cagagcttta ggggctaatc gatgggtctg tttagtaaaa attgagttac ctgcagccat
     9301 ccctagcatt gtcagtggca tcaagattgc tgctattact gcaacgggta cagccaccat
     9361 cgcagcattt gtgggagcag ggggttatgg tgagaggata gcgcaaggat tggcgaccaa
     9421 taatacgcag acgatgcttg ctggagcaat accagccgca atttttgcta ctttgataca
     9481 agctcttatc agcctctatg cgcgctttgt ggcccgctct cgagcctagg attcattgat
     9541 taataatgtc tgatcattgt cagtattctc cccacccaac ggctcagcag ctgcgagagt
     9601 ggatttatag tgcaaatgcc ctgactcgta aaataattga agggcttgcc ccaggaaaag
     9661 agctcgttcc acaacaagca actcttaatc caccgtattg ggagtttgcg cacattactt
     9721 ggtttcatga gttttggatt catcgcaagg gctgtcatga acgcccatcc ctgtatcagc
     9781 atgccgataa gttatttaat tcatcaaccg tagcgcatgc agatcgctgg agcattccac
     9841 ctctatcgat tgaaagtttg ttacggtata acaacgaggt atgcaataaa acaagcgagc
     9901 tcctcagtaa acccttaagt gacgaagatg cctactttat acagttagtg attcatcatt
     9961 acgatatgca taacgaagca tttgccatta tgtggcagtc acttgaatat cccatgcctt
    10021 ttgagccata cagtgtttca accaagacag tcttcaataa agagttcatt gagttttcaa
    10081 aaactgatat gactttagga tcatccactc agcaagggtt catatttgac aatgagaaat
    10141 ggcaaca
//
LOCUS       DDPQ01000016            5421 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_24105,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000016 DDPQ01000000
VERSION     DDPQ01000016.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_24105"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    287..379
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    967..981
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1525 a         1255 c         1118 g         1415 t
ORIGIN      
        1 aagatggtgt tgtatgacat ttttaatccc ttcgcggtag caataaccaa acacgaccat
       61 tttgcttcat tctgcctgct aattcaccag cctcatcacc agttccccag aaataatcaa
      121 cccgcactgg ccccacgatc gccttaccgg tatcttgggc catgactaag cgtcgtatag
      181 gctggttact taagggctgt gtagtttcta gaaaaaccgg cgctcctagc ggaatagcac
      241 ggggatcaat agcaatgctg cgtcctggac ttaatggcac acccaannnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnng gattacgctt tccccactcc gaaattcctt gcatcgtcgc
      421 ttctgcacgt gtaatttctt tacgatccaa gagccactgt gcactggatt taaagggttg
      481 gtcattagtt ccagcaaagc caaagcgcac gatctgtcca ccatcaaggc gaatctttcc
      541 tgacccctgt atctgcatag acgcagctgc aatagggtct tctacccaag caatttcaga
      601 gccctttagc aaaccagaac ttaccaactc tgcacgtgtt ggtgctggat taggctttgc
      661 tctcgcccac gttttaggat aagcatataa gggaacggaa tagcgctctg ttcgaacccg
      721 cgagccgttc ataataggct cgtaatagcc agtgaccagc ccctcgaact ttccagttac
      781 ctgattacga accgagtaag cccgaaaatg attctcaaaa aatgctttgg catctctagg
      841 cttttgagca ttggcacgat cacaaaccga aatccaattg accgggccgg tcttgcgctt
      901 tcgtagggca ttgcaacttt gtagccatgc ctgccaagca tcggcaagcg catcttcatt
      961 ccatccnnnn nnnnnnnnnn ngaccgtctc aatgaccttg ggagtgcgcg gtaaagatga
     1021 agacgccgat ggtccggagc tggattttgt agggggtgtt gcgcaagaaa tcaagagcaa
     1081 acttgccatt gcagcgatta tgggaacaac gaaggctgat ttgcgattta caattacacg
     1141 caggaaagag aataagtcca tgctcgctca attatttgat gaatacccga tgctcttgtt
     1201 tgtactagag ggactgcttg ccctagttat tctattgact attgtatttt ggacgggaaa
     1261 aggcagaaaa gactgaggct taatcagcca aaatcttaga taggaagtct tttgcccgcg
     1321 gggatcgggc atctggatta ccaaaaaact catctcgtga gcaatcctcc acaatgattc
     1381 ccttatccat gaatataact cggtgactca ctttcttggc aaatcccatc tcgtgagtaa
     1441 ccacacacat agtcatgccc tcttgcgcaa gtttcaccat gacatccaaa acctccccaa
     1501 ccatttcagg atcaagggct gaggtcggct catcaaataa cattaccatt ggatccatac
     1561 atagagcgcg cgcaatcgca acccgttgtt gctgaccgcc agacagttgg cctgggaact
     1621 tatccttttg ctctagcaag cccacgcgct cgaggtattt aaggccatgc tgtttagcat
     1681 cttctagaga gcgattgagt actttaattt gggcgatggt taagttttcc atgaccgaga
     1741 gatgcggaaa caactcaaag tgctgaaaca ccatgccaac ctttgcccgc aacttcggta
     1801 aatctgtttt agggtcatgc aaacgaatac cgttcacaat cagctcaccc tcctgaaatg
     1861 gctcaagggc attaatcgtt ttaatcaggg ttgatttgcc agatcctgat ggtccgcaga
     1921 tcaccacaac ctcccccttt ttaatagtgg tcgagcaatc ggtcagaacc tgaaactgac
     1981 cataccattt tgaaatatgt tgtaactcaa tcatttctat ttactttcta aactcatcga
     2041 atgatggcaa ctttttgctg aatcctgcga acgattctgg atagggtgaa acaaatcaag
     2101 aaataggtaa ctgcggctag taaataggtt tcaatcggac gcccaaagtt tttaccagca
     2161 atctcaaaac cttttaataa atcataggca ccaattgcat agactagcga agtgtcttgg
     2221 aacaaaataa tggtttgggt cataaagacg gggatcatat ttcgaaaggc ttggggcaaa
     2281 accaccaaac gcatattttg tgaataggtc attccaaggg cttggccagc atatacttgc
     2341 ccttttggaa tcgattgaat tccagcgcgt acgatttccg aaaagaaagc tgcctcaaag
     2401 gcaataaagg tgatgattgc tgatagattc accccgatgg gtttgccgat aatcagtgga
     2461 atcaacagga agaaccataa aatcaccatg acaagaggaa tggatcgcat ggtgttaacg
     2521 taagcagttg caggtaacac cagccatggc ttaccagaca aacgcatcaa tgcaagaata
     2581 gtacccacca aaattccacc aatcgttgcg atcacggtga gctgaatact aaaaatcaaa
     2641 cccttttgaa tatagttggt aaataaagcc caattataga aactaagatc aagattcatc
     2701 atgattttct tggtctctta atgtccagat gaattacttg acgcaataaa acccggaaca
     2761 cgtgtttttt tctcaatcca ggtcatcacc cgattgactg aaaatgctga aatcatgtac
     2821 aggccagtca ctgccaaata gatttcgata ccgcgtgacg tttcctcttg tgcctgcatt
     2881 gcaaagaggg taagttctgg tacagacaca gcaaacgcaa ccgatgagtt tttcacaata
     2941 ttcatacact cggaggttag cggtggaatg atgattcgta aacccatcgg taataaaaca
     3001 tattggtagc tttgcgctgt agtcatccca agagcaattg cagcataccg ctgccctctt
     3061 ggtagtgatt gaatgccggc tcgaacttgt tctgcgattc tggcagacgt aaataaccct
     3121 aatgcgatgc taaccaaaag ataggatgga aagctcttca taatcggaaa caaggccggc
     3181 acaacgtggt accaaaggaa tacctgaacg agcaatggga tgttacggaa aagttcaacc
     3241 caagcagtag caaaaccagt gaggatcttt tggatgaaat gattctgggg caacgtcctc
     3301 aaggtaccaa tcaccacacc catgatgagg gccaaaataa ggcttaaagc agcaaccgct
     3361 aaggtccacc cccaagcctt aaatagccaa tccaaatagg ttggatcaac attgggatcc
     3421 attccaaaca aaaaagagaa gcagcgcggg gctgcttctc cagtcaaagt gtctttacaa
     3481 aagacactcc aatctaaggt catataaaca ctcgctaaaa accttctgct tacttctttt
     3541 gataatcttc agcaggtctg tcatttggac tagaccaagc ttgtttggtg ctgtctgaga
     3601 gagctaaacc aactctggca tttttaggag gaataggctg cataaaccac ttatcccaca
     3661 attttgtcag agtgccgtct ttcatcattt taatgatgct gtcatccacg gctttcttga
     3721 aggttgggtc atctttacgc atcatgattg caatcggctc tactgcaatc acctcaccaa
     3781 caatcctaaa atcagctgga ttcttggcat tggcaatatt accggccaaa atagatccgt
     3841 ccatcacgaa cgcatcggca cgaccagatt caagcaagag aaaactatca gcatggtctt
     3901 taccaaatac ttcttcaaaa tttacgcctg ttgctctctc gtgtttacgt aatagctgta
     3961 ccgaggttgt tccagtggta gtggcgactt tcttaccatt taattgattt aaggaagtaa
     4021 tgcccgaatt tgcttttact gcaatccgaa tttcttcaac gtaggttgtt actgcgaatg
     4081 ccacatcttt ttgacgagtt gcattattag tggttgagcc acactcaata tcaacagtcc
     4141 cgttttgcaa taatgggata cgattttgtg aggtcactgg actatattta atatccaatg
     4201 ttttcaggtt caattgcttt tgaatatcac gcaatacatt gttgcaaatc tcaacatgaa
     4261 aacctgcata cttaccgtca cctaatgtgt aagaaagtgc accagaggat tcgcgcacac
     4321 ccatagttac cgctccgcta gacctcatct tgtctagagt gctctgttgc gcaaatgccg
     4381 caggggtaga aaataagccg atgattgata tagccaacgg cattaataaa gtttttttca
     4441 taaaaatcct ttaacaacaa attaataaaa acagcgaggt atatttttag cagaaaatgc
     4501 tcctgtaata accctaaaat tctaataatt agggttaatc cttagggcaa ttgcccctac
     4561 taaaaaggct ttaatgcaag attcttggca ttaccaaggc aaattctgga atatctgctt
     4621 ggaaaaattg agcgtcctca gttacacaga aatactcacc acgcatggta ccggctgggg
     4681 tgggcagggt tgcccaacta gtgtattcaa aatgctcccc agaacgcaat aaaggctgtt
     4741 gccccaccac gcccaaacct ttcacctctt ggacctcccc atcgccatcg gtgatgaacc
     4801 aatgccgtgc gatcaactgc acccccgaag cacctgtgtt tcgaatggtt acggtgtatg
     4861 caaaggcaaa ttgctgattt tcaggatcag attgctcagg taggtattgt ggccgaactg
     4921 ttacggtaat ttctggtgga ttcatgaaca gcattctgct ctatccatta cctgttcgca
     4981 agctagaatc taggtatgac aagccaaaaa gccctaaatt aacccccata tgagtcaaat
     5041 aagcccagtt attgcaccct ctatcctgtc tgcagacttt gccaagttag gtgatgaagt
     5101 aagagccgtc ttggatgcag gcgcggactg gatccatttt gatgtgatgg ataaccacta
     5161 cgtccctaat ttaactgtgg ggcccttggt ttgcgaagcg attcgtcctc atgcacttaa
     5221 aaatggtaag cctgcaatga tcgacgtaca tctcatggtt gaacctgtag atcggatcgt
     5281 gcctgatttt gctaaagcgg gcgctaattt aatcagcttt catccggagg ctagtactca
     5341 tgtgcatcgt acattgggtt taattaaaga taatggctgt caagcgggct tagttcttaa
     5401 tcctgccact ccaattcagc a
//
LOCUS       DDPQ01000017            2807 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_29501,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000017 DDPQ01000000
VERSION     DDPQ01000017.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_29501"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    400..428
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1423..1513
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          718 a          573 c          617 g          779 t
ORIGIN      
        1 tatgagcgca cagtcgcatt tgccatccta tttatcaact tctagctatt ttgctaatca
       61 attgttgtta gcaatgccag gaatgctcga tgataacttt gcaggatcgg tcatctatct
      121 gctcgagcat acggataagg gtgcaatggg attggtggtc aacagaccca cggagctcgt
      181 tctctctacc ttatttgaaa agattgagct caaattagaa attgcccctc ttttagatca
      241 gccggtctac tatggcggcc cagttcaggt agagcgtggc tttgtattac atcaaacaaa
      301 ctctttggag aaatattctt catcccttgt cattgcggat ggcttaacga tgaccacatc
      361 aaaagatgtg ctcgaggcag tcgctagcgg aaatggcccn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnga aatagcatta aatggctgga tgaatgttcc actatctagt gtggaaatga
      481 gtaagatcat ttttgatacc ccatatgctg aacggtatca gcgagcaata caaaatcttg
      541 gttttgatct atcaagcctt tcaggagagg cgggtcatgc atgattcctc tcccattcat
      601 aaacctttaa gcgtgatggc gttcgattac ggtaccaaac gaatcggtgt ggcagttggt
      661 aactccttga ctaagtcggc acagaacttg ccggttattt tgaacgctgg cgaagagcag
      721 cgatttgctg ctattgcaaa gattattgcc aactgggctc ccgatgcgat tgcggtaggc
      781 ttacccttgc atccagatgg ggctgagcat gcaatgaccc aaaaagcacg gcgctttggt
      841 cagcaaatag gagggcgtta ctctaagcca gtttattttg tcgacgagcg ctactcctcg
      901 gtagttttag agggcgatgt tcaatatcag gaatcgctag actcccatgc ggcggccctt
      961 attttggagc agttctttag ggaacgaaaa atttaggaat cagcgcaata aagcattaac
     1021 ggctttgaga tcctcatcag aaagactggc tgcttgagct tttaacttaa gcgcattcat
     1081 gatcgtgtca tagcgagcct tatacagcga tgctctggtt gtaaacaagg tatccaaagc
     1141 aataaggaca tcaatattga ttagggtgcc aactcgataa cctagcttac tggactctac
     1201 tgcagaagct gaggatttct ctgcagcttc tagggctttc actgtggcta aaccaccata
     1261 aaatccagtg aatgcttgac gagtcgcctg ggccgctgtt cttctaaggt tatcgtagtc
     1321 tgcctttgcc ttatctgcca gagcagcatt ttgccgaatc acagacatat tgaagccacc
     1381 agagacaatt ggtaaggtca tttgtagggc aatcgtattg ttnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnacgagaa cgttatagct ggcattttca gattgcgtaa tccaatcctc
     1561 tagggcttgg cctggcggaa gtttaggatt aacgctctca gcaataggca cacctttttc
     1621 atctcgattc ttggatctgg gatctgcgac caccccggca atcgatatat cttttgccat
     1681 tggtttaatt tgttttactg gatgaccaat cagctgttca agaacacccc gtttcacaac
     1741 aagttccgca tgcgcagcaa tctcttgagc gttggcaaca tcaaagcgtg cttgcgcatc
     1801 attagcatcc acaatagtcg ctgaaccaat ctcaaactta gcctttgcag cgtctagctg
     1861 ctctttgatg agtcctttct tattcctaaa taactcaaca ttatcttgag cggttagaac
     1921 ttcaaagtaa gcttgagata cccgaatgac taaatcttgt tgggcctgta aaaactgtaa
     1981 atcagaaatc ttgctattta gatcaccttg tttgtagatt tgcatgcccg ctagattaaa
     2041 gacgggttga gtcaaagtta ctgtgtagct cttttgatca aaaatgcggt tagtaccttc
     2101 accgtgctga aaaaaacggg tccctgttgg attggcagat acttgcggta agagtaccga
     2161 taagccttgc caaaatagtt ctttgcgagc agtaagatta aagcgagcac tattaattac
     2221 cgggtcatta aaagcagctt cttggtatag actaatcaaa tccataacac gaccatcttt
     2281 gagtcgatcg ctctttccat ctaccaaatt tgagttcacc ggtaatgccg gaattgcaac
     2341 ttgtggggga atctctagtt tgatcaagtc ttgggggcta agtgtttttg ggagaataga
     2401 gttattcgct ggcgtcacgg ttacggagcc ctgcgagccc attatgttgg gtgacccctg
     2461 actttggcca tagctaatgg aacaggggag caagcccatt acaagaagca aaaaagcagt
     2521 tgaaataaaa ggcttattca tgaacatgat ttgaagttta agcccaaaat ggtttattaa
     2581 taggtttggc taacgaaggc tttagtattg atacccatgg acccgttact attatttaaa
     2641 gccctcattt taggagtagt tgagggcctc actgaatttt tgcccatatc tagcactggg
     2701 catttaatct tggtgggcga cctactaaac tttaatgacg aacgtggtaa agcctttgaa
     2761 atcattattc agttcggagc tattttggca gtgtgttggg agtatcg
//
LOCUS       DDPQ01000018            6546 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_14523,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000018 DDPQ01000000
VERSION     DDPQ01000018.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_14523"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    5410..5448
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6306..6398
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1671 a         1499 c         1496 g         1746 t
ORIGIN      
        1 gatgcttata ttgaatggca ggaagaagag gtcattgatt ttctaatcga ttattgggaa
       61 aaggctcgta aggtaggtct aggggttccc agtgactttg cggatttcta tcgtgatttt
      121 gagtggatgg gattacaacg acacctcaaa atacttggca tctttgcacg actgtatcac
      181 cgcgatggta aagagaatta cttgaacgat attccgctgg tactgaaata tgtacgagcg
      241 gttgctactc gctatattgc tttgaagcca ctattacgta ttcttgatcg agtagaaaca
      301 aacaaatcat gaatgctaca tccgcgttaa ataactttga tcctattcca tgcctagtat
      361 tagcagccgg acgtggagaa cgaatgcggc cattaaccga tactatccct aagccactat
      421 tgcaggttca aggtaaatcc ttactggctt ggcatcttga agctctagcg aaagctggtc
      481 ttactcaggt cgtcattaat catgcttggt taggcgaaca aattgaggca agcttaggct
      541 ccggtaagca gtttggtctt caaatctttt actcccgaga agaatcagca ttagagactg
      601 caggtggtat ctgtaaagca ctaccactcc ttagagctaa tgactatttc ttggtgatta
      661 atggtgatgt gtttagcccc cactttccaa ttcagggctt aatccaacga gttcttgagc
      721 ttcgttccca agatcaatta ttagcccatc tgattatggt tcccaatcca ccccaacacc
      781 cgcatggaga cttttatctc gaccaaacaa tggtcagaga tgatggatat acaggggcta
      841 cgagtgcaaa actgactttt tctggaattg gtatttatca ccacacccta tttaagggcc
      901 tcatttctgg gcaaagcgct aaattggctc ccatattgcg caaggccatg gcagataatc
      961 gggtttctgg tgaaaaatat ctgggttcgt ggcacgatgt aggaagcccc gaacgattag
     1021 ctgaacttaa taaactataa accgactatt gccatgaatc gtctgtcttc tcataaccct
     1081 caggttgctg cctgccagca acggcgaact cgcttggcac aacttattca ggccaagagt
     1141 ggtggtggtg tagtaattct ttcgacggcc gaagaaaaag ctcgtaatcg cgatagtgat
     1201 tttccttatc ggcatgacag tgattttttc tatctcactg gttttgatga gcccggcgca
     1261 accttggtca tgctgataca gaaaacaagt tttgagacct atttattttg tcgccccaaa
     1321 gaccttgaac gtgaaatctg ggatgggttt cgcttaggtc ctgaagcggc tcccgatatt
     1381 ttgcatctgg atgctgctta ttctagccaa gaacttaatc agagactccc cgagttccta
     1441 gccaatcaat cagcnngtgc gtcaacaagc aagatcgggc accaatcctc ccgaggtctt
     1501 tcacgatatc gatgcactcg ttcatgagat gcgtctctta aagggtcctg aggaaatcga
     1561 gaccatgcgc caagcggccg ctatttctgc agaagcccat attcgggcta tgcgctcatg
     1621 caaacctggc aagcgcgagt atcaactgga agcagaactg ctccatgagt ttcggtatcg
     1681 tggctcccaa agcgttgcct ataacagcat tgttgctgcg ggtgctaata catgcgtctt
     1741 acaccaccgc gcaggctctg ctgaattgct cgacggcgag ctttgtttaa ttgatgcggg
     1801 ctgcgagcta aacggatatg catccgatat cacacggacc tttccgatta atggccgcta
     1861 cagtgctagt caacgcgcct tatatgacat taccttggcc gctcaggaag ctgcggtttt
     1921 agaaactcgc cctggtaaga ccttcattga tcctcacaac gctgcggttc gagttctcac
     1981 ccaaggcttg cttgatgaaa aactcatcaa actcagtgat gttggttctt tggataatgc
     2041 gattgagtta ggtgcctata agcggtttta tatgcaccgc accagccact ggctaggtat
     2101 ggatgtacac gatgtgggct cctatcgaga tcctctaatc agtcaacaag ctggccaaga
     2161 gaaaccttgg cgcctcttac aaaaaaatat ggtactgacc atcgagcccg gtttatatat
     2221 tcgtccggct gacgatatac ccaatgtatt ttggaatatt ggaattcgga ttgaggatga
     2281 tgcggtagtt accgaccaag gctgtgagtt gatctcgcgt ggggttccag tggatcctag
     2341 tgagattgaa gcaattgttc gctcctaata ccatttcttc tatgagcaat tcatatgctg
     2401 tggtgattca agggggtggt ccggttgggc ttgcttgcgc agcctggtta ttgcaaaaga
     2461 actccggctt atcactccta ctcctggatc gcaatcccgc agatgaccaa gccatgaaag
     2521 caggagatgg gcgtggcatt gccctatcgc atggcagcaa actcctcttg gacacgattc
     2581 atgcatggcc cgatcactgc cccgccattc atcaaatcca tgtatcgcaa gttgggcgtt
     2641 ttggtcgtgc cctaatgact cgagaagagc tcaagcaaga agccctaggc cacattagcc
     2701 gctataccga tattcatgta tcgcttagaa aggctttgcg gtctatccaa aagcatgccc
     2761 ctcattttga gtggcagcat gatgttggcg attctgcacc cccctttgag acagattgct
     2821 tggtccacgc cgaaggcggt ttgttcaaag accaagattg gaccgaatcg ggtcgcgatt
     2881 atgagcaatc cgctctggtt gctacggttg aagtagaaaa ggcaatcccc catcaggcct
     2941 gggagcgttt taccaatgaa ggtcctttag cccttcttcc aagtcatcta gggcctaata
     3001 ccctcaattt agtatggtgc gccactcctc atgctagtca aagacgttta gatctcaatg
     3061 acaccgaatt cttaaacgaa ctacaaaatg cctttggtca tcgcttgggt tcatttaagg
     3121 caatagcaaa tcgtcgtctc tataacttag gcttgaacta tcgcaaacat gtttctgagg
     3181 gtcatgaggt ttggattggt aatgctgccc aaactcttca tcctgttgct ggtcaaggtc
     3241 ttaatcttgg cctgcgtgat gcgtatcttc tggccgaaaa acttgctgaa ttatttaaag
     3301 caaacaatca atcgctaaat cgagtactaa ataaaataca aactcatcgc gcatctgaag
     3361 aaacaatcgg tgccgtacaa aatgcactgc aagaatatgc aaagagtcgg gagattgatc
     3421 ggcgtgcaac gattggcatc acagatttct tggcaagagt cttcacctca cccatcctgc
     3481 cagtacaact ttcacgtggt ttagccttaa ccgccttgca atggatgccg cctatcaaaa
     3541 cagcactagc tagacaaatg atgtttgggc gacgctaaca ccctaattgt atttgcttaa
     3601 aaattaggca gcacaggcgt gcctgtgata aagtgttggg tctattccaa gaaaaaattc
     3661 cacacgcatg caaatcggtc atcacaaatt agccaatgcg ctttttgtcg caccaatggc
     3721 tggggttaca gaccgaccat ttcggcagct ttgcaagaaa ttaggggctg ggtatgctgt
     3781 ctcagagatg attgcctcca atgccatcct ctggaatagt gaaaaaaccc aacggcgtgc
     3841 taaccataca ggtgagttca aacctattgc ggttcagatt gctggggccg atcccgacat
     3901 gatggctgct gctgccagac tcaatgttga tcgaggcgct caaatcattg atatcaatat
     3961 gggctgccca gctaaaaagg tttgtaatgt tgctgccggc tcagccttac tcaaggatca
     4021 gcccctggtt caacagattg ttgaggcagt ggttaatgcc gttggcgttg gccccgatgc
     4081 agttcctgtc actctcaaaa tacgcacagg ttgggatcga gaacataaaa atgctctcac
     4141 aattgcgaaa attgcccaag atgccggcat cagcatgctt acgattcatg ggcgaacacg
     4201 tgctgatttg taccatggtg aagcggagta cgacaccata gcggctgtaa aggaatcagt
     4261 ctgcatacct gtcgttgcta atggtgatat caacaccccc gaaaaggcaa aatgggttct
     4321 tacgtatacc ggtgttgatg ccctgatgat tggtcgtgct gctcaaggaa gaccatggat
     4381 ttttcgggaa gtggatcatt acttgctcac cggaaacaaa ttacccaatc ctcaaattgc
     4441 agagatccaa gccatcatga acgaacattt gctcgatcat tacgcatttt atggagaata
     4501 tacgggctta cgaacagcgc gtaagcatat tggctggtat tgcaagggtt tacgtaactc
     4561 acattccttc cggcatctca tgaatacagc ggatgactgc aaaacccaat tgcaaatggt
     4621 caatgactat tttgatgaaa tgaagctcca ctccgatcaa ctcctttttt tagaagcagc
     4681 ttaagtttta tagctatgcc aaacaataaa catcccgtca ccgaatgtat tgagcttaat
     4741 cttcagcgct accttgatga tctcaagggt acccccccaa gcgatatcta tcaaatggtt
     4801 ttgagtgtag tagaaaaacc catgctggag ctcgtgatgg ctcatgccaa acaaaatcaa
     4861 tccctcgctg ctgcttactt aggaattaat cgtaatacct tgcgcaaaaa acttcttgaa
     4921 catcgactga tctaaattat gattcggacc gccctacttt ctgtttctga taagaatggc
     4981 attgtggaat ttgcccaagc ccttcatcaa ctcggtgtga agttaatctc tacaggcggt
     5041 acggcaaaat tacttgctga taaaggtctt cctgtcaccg aggtctctgc gttgactggc
     5101 tttcctgaga tgcttgatgg tcgtgttaag acccttcacc cgaaggtaca cgggggacta
     5161 ttggcacgtc gcgactctaa agaacatatg gcagcaatac aggagcatgg tatcgatact
     5221 attgatctct tggtgatcaa tctgtacccg tttactgaaa cgatttctaa gtctgagtgt
     5281 agttttgatg aggcggttga gaatattgat attggtggcc cagctatgct gcgtgctgct
     5341 gctaaaaatc atcaagacgt caccgtcctt gtatcaccgg atgactactc ttccgttcta
     5401 gaagaaatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat tgctaattac
     5461 ctatcttccc tcaatgaaga gcacgagcat gccaagcgca gtccttatcc acaaacactt
     5521 cacctagcat ttgagcgggt ccaagaaatg cgctatggcg agaatccgca tcaatcagcc
     5581 gccttctatc gggatatcga agcgccgacg ggtagtcttg ctcattacaa gcagttacaa
     5641 ggcaaagagc tgtcatttaa taacattgct gatgcagatg ctgcctggga gtgtgtgaag
     5701 agtattagtg atagcgaagc tgcttgcgtc attattaaac acgccaaccc ctgtggtgtt
     5761 gctattgcta gtacggctct tgagtcttat caaaaggctc ttcagaccga tccgacctct
     5821 gcatttgggg gcattattgc ccttaatcgt gaatgtgacg aggcctgtgc gcaagcgatt
     5881 gccaagcaat ttgttgaggt cttaattgcg ccccacttta gcgagtctgc aaaagctatt
     5941 tttgcaagta aacagaatgt tcgtctactc gaaatcccac tgggtgaaag cgtatcaagc
     6001 cttgcacaat atgatctgaa gcgcgttggc ggtggtcttt tggtgcaatc gagtgactct
     6061 aaaaatgttc ttcccagtga gttaaaggtt gttacaaaac gtcaacctac tcccacagag
     6121 cttacagatc tgatgtttgc atggcgtgtg gctaaatttg ttaaatcgaa tgcgattgtc
     6181 tactgcgcca atggcatgac cttaggagtt ggtgccggcc aaatgagtcg tattgattct
     6241 gcgcggattg ccagcattaa ggctgagcat gctggtctta gcctgaaagg ctctgctgtt
     6301 gcgagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tgcaaacgaa catggtattg
     6421 ctatggtctt cactagcact cgccatttca ggcattgatg ccgtccatgc gctggctagg
     6481 tatcgatcca ggtttgcgca ccactggctt tggtgtaatt gaacttgaag gtcagcactt
     6541 acgcta
//
LOCUS       DDPQ01000019            6548 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_27112,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000019 DDPQ01000000
VERSION     DDPQ01000019.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_27112"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    144..211
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2262..2400
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3125..3213
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4220..4315
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5313..5403
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1846 a         1387 c         1250 g         1582 t
ORIGIN      
        1 ctggttgtat aaaacctgat cttgctctct cgcatgaccg tataaaagcg ttggctgcgt
       61 atgcgtgact ctgaaatgaa atgggttgcc ataattttta cctgttgtgc cctggtaagt
      121 atagataacg gctatatcct gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattcactta atatggtttt ttctgggttt
      241 tctggcgctt tctggagttt tttaatgaga gcccaatttg gtcaatccac ccctatttga
      301 agatttttaa gtaagtattt gatttgtatc ccaataattt tgtagaatcg aaccatattt
      361 aactgcctcc aaaaaggatt aagaaatggc tgacaacatc aaacctggct ccctctacgt
      421 tggcgagggt gtcattatca aaggtacctt tgatgttcca gacatggcag caattgccgg
      481 cctcgttgag ggtgagttga ccaccaagca ggtcttagtc gattcctcag gggttattaa
      541 cggtaaggtg aatgccgaga ccgtcgaggt gcgcggtgaa attacgcaag gtctgtcagt
      601 gaccgatcac ttaactgttt acgcaacggg caaaattacc ggcacgatcg agtacgcaca
      661 gattagtgtt gaaaaaggtg ctcacttaac gggtgatctc aaggttatta gcaagacaac
      721 gtattaagta tttgggcttg caatgctttt tgttgcccca atacatttct gaaagtacct
      781 cccccgcttt ttcattggaa gtcattcttc cattctgggc attaaagcag taatgcccgc
      841 cagctttctt ctagaataaa tccatgaaga tcgctcttaa aacccagttt ggccagcatc
      901 ttgcgctaac cccgcaattg cagcgatcga tccagcttct ccaaatctct tctgctgaac
      961 tcgaagaaga ggttgctaag gcggctgagg aaaatcccat gttggactac tccccgcctg
     1021 atcaatcgaa agacgatggg gaaccaaacg aaaaaatgga aatgatccaa gccaattggg
     1081 catcgagcgc accatcgcgc gatgacgatg aagattggga tacgcggcac gagcaacaag
     1141 cccaggtcaa gtcgctcctt aattacctag aagaacagat acagtcattg cggatcgatc
     1201 ccccagtaaa gggattggtg gtttacttgg cgggctgcct ggatgagcgt ggttatttac
     1261 ccgagtcgtt agaagatcta gccacagaaa tccattcaga gctaaaggtc actaaggcat
     1321 ccgccctcac acagctcaaa actgcactaa ctgagttaca aaaattggat ccggctggca
     1381 ttggcgcaca ctcactcgca gaatgtctat gcctacaaat acagcggcaa atgcagttcg
     1441 ataccgagca ctccgacaaa ttacagcttg cattgaactt agcccaaaac catcttagta
     1501 agattgcgca aaaagattgg agcaaattac ggcaaatctt taagcagtcc gaggcaaata
     1561 ttatggaggc cgtggcgcac atcagaacgc tccaacataa cccaggcgaa cattttgata
     1621 ccaatgttga gcaatggatt agccctgatg tttttattaa gctaaacgca aagggtaagt
     1681 gggtggtgca ctctaatcaa gcaatcaagc ctcgcctgac actcaaccaa gaatacagtc
     1741 gcattctgaa agaaaataat gcgaagaaaa ataatttagc gcttcaccaa aaaatgctgg
     1801 aagcccgctg gctgattaag aacattgagc aacgcgagga aacgatccta aaagtagccg
     1861 aggccatcgt agagcgccaa cagaatttct ttcaccaagg caatatcgct atgaagcctc
     1921 tcgtcctgcg agaaatagca gccgcaattg gtatgcatga atcgaccatc tcgcgggtca
     1981 ccaacaacaa atatttagcc tgtcctgccg gtatctttga gttcaagtac ttctttagcg
     2041 cacaactaag ctctgaaaaa ggtcaagctg tatcggctac cgcagttcaa tccctaatta
     2101 aacaaatcat tttggacgag acccccaaaa aaccgatttc tgataatcag atcacaaact
     2161 tactggcaag tcagggcttt gtggttgctc gcagaacggt cgctaaatac cgtgagctct
     2221 tgcggatacc cccagctcac cttcgtaaga attgaagaat gnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 atgcgtatga aaaagagctc gctgaaatag aggccaataa gcttgaaaac aaaatcataa
     2461 aagcgcgctt gtatcgtgaa aaaaatccgc tcgataacca ctacgtcaaa ttaacaagca
     2521 atcactacaa ccgcatacag gtcaatgtga atgatggcaa gttccgaacc tttgtgactg
     2581 ctgagattgt tttaaaagtt cagaatagtg cgactgccag caacctgaaa aatgtgctac
     2641 cagtcatcga acacaaggct aatacattgc tttcttctat tcccgcccaa gacttaagat
     2701 cggcctttgg aagaaatgca tttactcagc agatcttgct ctttgccaac gaccttctcg
     2761 accccgagct aaccactttg tataaaaatc aatttaatgc aacccctgca aaggcggggg
     2821 acaataaatc tgaaacggat tctctgcctt ttgagattac taccgaagac ctaccagttc
     2881 aggcagtact ctttgaggcc ttcgtgatca accgttaagg cttcacccaa cagcaaggca
     2941 aagaggtcta ctctaaaact tcccacttgc ctgctaattt cttgtgatag ggctcgtgat
     3001 aacgaagtaa aacaatatca ccggcaatcg tatcgacatt gatgatgcgt ttacataccg
     3061 atgaaatgac ttcgcgattc caggcacgat caggcttttc tagccaagca ttgattcctt
     3121 tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgggctc tacttttatc tttttctaaa
     3241 ggaatctcaa tcacaatgta tggcaatgca aatgccaacg cacggtgttt acgaacattt
     3301 ctaaaataaa cttgcttggt gattgcccag gcctcattag agtccatcaa agagccggaa
     3361 aaggtccatt tctttgacca aaagaacata cattgcgact cctgcagttc tttaataatt
     3421 gttttaggga ttgctttatt catgccatat tcaaatcaag ccatcgtatg gtggtttatc
     3481 ttgccgaact catattaaaa ggatcaaaaa tttagctttt ttatcagccc caatagcgat
     3541 caatggatct ttatgacaat gatttaaaag ggtaaatagc tatttgctat tattactcaa
     3601 actgccttct aatatgcact aaaacgaagt accaactaaa gatcgaggtc aaacacatgg
     3661 aatttataaa actattaaaa gcgggttcca ttattagtgg catcaattgg gccatttatt
     3721 tgattcatga atgggtcaat tttgctaaga ataatccccc agaggcactt agtgagggag
     3781 atcccatatt cttaggtttg atcgctcaat caatcgccag ccttggttta ataggatttg
     3841 gagcctttta caagggtagt aactcgaatg ctaataccga ggctaacaag gttcgctcca
     3901 agccccattt aacagctcct ccaactagct ttagcaagcc caatgaaaat gcggtgctaa
     3961 accgaaccga gcctaccgca acacctgttc agaatggttt tgccgcccca gccatgccag
     4021 cctcggtggc atcgccaact gcacccccgc cctttgcacc gccgccccct gtcactcctg
     4081 ccaagcccac cgccccagaa aaatcaagtc cgacgcggac aacagcacca aaaccatccg
     4141 ataccaacca tcgcctaatt acgcttattt tgattgtgat cggactaatt ggagctgcaa
     4201 tttattacgt caagcaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgac
     4321 taatttagaa acaaagacgg aggtaccttt cagatggctt ggtagtgaac catcggaaaa
     4381 tccaatggag ccaattttct attacggcaa gactgtcagt aaagcgaatt tattaagcaa
     4441 ctttgagaaa tataccaacg gcatcgccaa tcctagcgat gagcaaaaga atcagattga
     4501 gcaatctaaa aagattttgg atgatttaag tgatggctcg tatcgaatca ttcgtttaaa
     4561 aatgagttgg agtgctggca tctatcgttc ctatctattc gataaaaata gtatctttaa
     4621 attagacttt gagtccgaag aatcactgag cggtattacg cgctataaga aaactaatta
     4681 agctacctag ggcgccctcc tagggcgccc atctagaccg gggctgtaaa tgtaatcttg
     4741 aacactgcac cagtattttg cagactgtca tactgaatcc ggccaccatg gcgcgtcaca
     4801 atgtaattac acaaccatag gcccaaaccc atcccctcgc gcttagtgcc tgtaaagagc
     4861 tcaaacaagt atttctgctg atcaactggc acgcctaagc cattatcctc aaactgaatt
     4921 tcagtgatct cacctttctg ctttccaact atgcgaatgg ttttggaaga tggcttcgag
     4981 ctatctagag cctgaactga attgttaaat agattgatga acaattgctg cgcctcccca
     5041 aaactcatgg ggatttgcag atttttttct atgtccatct ctacatggat ttgcttctga
     5101 ttgagatcag attgagacaa gagtaagaca gcgtcaatga cctctctaac agagacgaaa
     5161 tcaggaattg catcgtattt ttctgcaaag atcgctttta gcgtttggat aatccgtgcg
     5221 gcacgcatat tatctttagc aatatcgttg gcaattcgcg taataagctc acgatccaag
     5281 ttctcagcct gcaacttctt ctgcaaaaac tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnctcattt tctgattccg tctgtcgacc atcaaaagcc aaacgctcag accaatatcc
     5461 ccaaatagca ataaaggata tggtgttcat taggatttgc cctaaagtaa agaggatcaa
     5521 taaaagaggt aactcatcca ttgagcgaat gggatggtca gaacttagca atatgattaa
     5581 tcggggaact aagaaaaggg cttcgccgac agctgcatac tgaaaatagc gtaattgctg
     5641 taattgcaat ttgcgattaa atagaaatgc ttcccgaatt tgccaagcaa acaccaaggc
     5701 ataaactaag accgcgaata tggttcgggc ctcaaaactg gcataaagtc tcaggtactc
     5761 aaaaatcagt ccatacacga tgatcgatat gaaaagaggc catcggagat gctcggatag
     5821 ttctttattt agagaataaa agaaggctcc ctgaaaaatt gctgctgcat aaaagaatgt
     5881 attggcaatc gtaaatatcc ccggcggttt ttgaatatct gcagtaaata aaataccaat
     5941 ggcaaataac aaaagaccgc taacgttaac gacaagagcg gctagccagt aggtattagg
     6001 tttagagcga ctcttggggc cgatgtagtg agaaatgcca atcaacaagc caagttgtat
     6061 cgccgcaaag ataaaaaaat agatcgacag aagaagcttc atgcgccaat cgtacccgat
     6121 ttaaatcatg caagattgga tcgtatgatg cgatgtctgg atggtgaatt taactctgcc
     6181 ttggtagcca aacccaaagc acctagagaa atccatcttt gtagctggcg gagagggtgg
     6241 gattcgaacc cacggtagac ttgcgtctac gcctgatttc gagtcaggta cattcgacca
     6301 ctctgccacc tctccgactc tccttgaatt atacctagcc cccgtgctca atctaaaaaa
     6361 gtattgacca gagtactgta ttcatctaat atgtagatac attaatagat atacattttt
     6421 aggaaatggt ttaattatat gaatattcaa atctctaaat ggggtaatag ccttgccctt
     6481 cgtattccca gtgaattgct gaaagaggca caactcaaag aaggtgactc tgtcagtgcc
     6541 actatgac
//
LOCUS       DDPQ01000020           13607 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_8750,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000020 DDPQ01000000
VERSION     DDPQ01000020.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13607)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_8750"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1537..1648
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4960..5042
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11310..11349
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12863..12918
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3692 a         3025 c         3125 g         3474 t
ORIGIN      
        1 atctttctaa agagggcatg accattttgc tagttgagca aaacgcacgt ctagccttat
       61 caatggccaa tcgagcctac gtgatggaaa gtggtgagat caccatacaa ggccccgccc
      121 aagagttact tcatgatgag cgggtacgta aagcttattt gggcgaagaa ttacaacagt
      181 aatcgagtgg cggtattggc gcttaaacca cttttttaag aagcattctt aagatggcgc
      241 acatttgccg aatttcatcg tttgaaacgg tgagggcagg cataaatcgc aacagatcag
      301 ggcgcgggga attaatcaat aagccctcgg gttcaagatc tctagctaaa tcaactaact
      361 ttccaccgat atccttgccc agcattaagg cacgcagtag acctttaccg cgctcacctt
      421 gtagaccaaa ttcagaggag agtttgagta attctgattg gagtaattcg cctttggtga
      481 tgaccgattg caaaaagcca ggagccaata gctgatcgat tacgctaata ccaaccgcag
      541 tcattaaggc attaccgtta taagttccac cttgatcgcc tggaacaaaa caggcaacat
      601 catcggtgca cagcagtgcc gccaagggaa cgccccctcc aattcctttg cctaaggtca
      661 tgatgtcagg ttcaatgccg taatgttgat acgcaaataa ggtgccggta cggccaaagc
      721 ccgcttgaac ctcatcaacg atcagcagta gattattctc ttgtgtcagt tggcgaagct
      781 cgcgcataaa tgaatcatcg caagggatga ccccaccctc accctgaact ggctcaagca
      841 tgatcgccac agtcttatcc gtgatgcatt tcttcactga ctctatatcg tttaggtttg
      901 cttttggaaa acctggaact tgaggcgcaa acatcgtatc ccagccaggc ttacccgagg
      961 cgctcatagt tgccaacgtg cgcccatgga aactatggtc aaaagtaata atttcgtagg
     1021 caccattttt atggagttgc ccccactttc tggcgagttt gattgcaccc tcattagcct
     1081 cggcaccact gttcgcgaag aaaactttat caaagcagct atttgcggtt aagagatccg
     1141 ataaacgaat catcggcccg ttgtaaaaag cagggcttgg attaattagt ttttgggctt
     1201 gttgatgtaa ggcgttcacg accccgggat tgcagtgccc caaacaattg accgcccaac
     1261 cttgtaagaa atctaaatag cgcttgccat tttggtcggt aagccacgag ccactccctt
     1321 ccaccatgac gatgtcggga cgtttggtga tgtacatcac cgaatgggcg tcaacaggat
     1381 gatggtcagc tggtttgttc atattgcaat catacaaaag atcggtggaa aaggggcatt
     1441 cgccctataa acccttactt aggcgcttat tagatcggcc tcactagtga aggaatcggc
     1501 aaaaaactcc tcttcgggca gacggcactc tttgacnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg taatgaaagc ccgggatggt gtgggtaaat
     1681 tcttggcaga gagaatccat gtataaatcc tttgggcgcc taccacccca gtaaaaatgg
     1741 atctcccgat caatcttctt attttggatg gatgtcagca tcgctttaat gggcgcaaat
     1801 cctgtgccgg atgctaaaaa gataattggc tttttagagt cttcacgcaa gaagaagcta
     1861 ccaagaggac cctcaaagcg caaaatatcc ttttctttaa tttgtttgcc atccgctgtc
     1921 tgtccaaata ggggatcagt aaataaacca ccaggcagat gacgaatatg caattcgagt
     1981 ggcccctcct catggggtgc gctcgcaatc gagtaagcac gacgtttatt gtctttgagc
     2041 aaaaactcta aatattgtcc cgccagaaac tgaaagcgct cgctagcagg aagttgcaat
     2101 tgcaaaatgg caacatcatc gctggcaaac ttaatagtgt taatacgaca ggggacctta
     2161 cgaacgggaa tatcccctcc accctgaact tcccgaactt caatcagcag gtcagtctga
     2221 gggtgggcac agcataaaag agtggcgccc gcagtttcat cggcaggact cagagcgctc
     2281 tgactatggt ccccatgggt aatttttcct gtaaggagcc ttcctttaca agagccgcaa
     2341 gctccatttt tacaaccata gggaaggttg atgctttggc gaagagctgc ctctagaacg
     2401 gtttcatcgg cttccaccga aaatgtttta ccactcgttt ttagctcaac ttggtaggac
     2461 aattccactt ctcctaataa aacattacga tgttagatat gacctctctc tgttccgcat
     2521 ttcgtcgtcc tagaatcctc atcattgggt gcggggatat tgggcagcgc gttgttacgc
     2581 aactactgtc aggcccgaat gcccatctag gtaaaacgta tgccaaacca aaaatctttg
     2641 ccctcagctc aagcccagag cgttttgcag agctgcgaca gcagggaatc acacccatcg
     2701 ggggcgacct tgatcatcct gagactttat ggcgaattgc ccgcttagca tcgaatgtga
     2761 ttcatttggc gccacctcaa aatgaaggtg aacacgattt acgtactcgc aacctaattc
     2821 aaattttatc tcagggggcg ggatctgtca gacgctttgt ctacgtcagc actacagggg
     2881 tgtatggcaa tcgtcatggt gattatgtgg atgagagcag ccctcctttg gcaaccagcc
     2941 agcgtgccaa gcggcgtatg gatgcggaat ctgttttgcg acattgggca attcatcagg
     3001 gtgtcaatct aagcattttg agggtgcctg ggatttatgg ccccaatcgc ttgccgattg
     3061 agcgattaaa tgcaaaaaca cccgctctta catctgaaga ggatgcatat tccaaccaca
     3121 ttcatgcaga tgatttagca agactcatcc ttggagctct ctttattgga aaacctcagc
     3181 ggattgtaaa tgcgtgtgac ggcagcgagc aaaagatggg agattatttt gatgcagtag
     3241 ctatggcact gaatttgccg aaaccacaac gcttatctcg agaagaagtc aggactgcag
     3301 taagccctat gctatggtcc tttatgtctg aatcacggcg cgttcgtaat acacgactgc
     3361 ccgagttaaa acgtaaatta ctctacccga cagtggctga ctacctaaag actcttaaaa
     3421 gatagtaatt atttccagct atcttttagc ccaacgctgc gattaaaaac aagctgacca
     3481 ggtcggctat ctattcgatc agcaacaaaa tagccatgcc gctcaaactg aaactgttca
     3541 tctgctttgc acgtgctcat gcagggctct agataagctt gaataacatc ctttgattga
     3601 ggattgataa agtctaaaaa gtttttatcg ccactatcag ggtgcgcatc tgtaaacaga
     3661 tgatcgtaga gacgaacttc tgctggcacc gcttcatctg tgcgtatcca atgaatgttg
     3721 ccctttacct tatagttatt agaatccggc gtgccacttt tgctatcggg gaagtaattg
     3781 gcataaacct cgacgacttg attattagtg tctagtttca tgccgataca ctcaataaca
     3841 aagccgtgtc ttaggcgaac tcgcccacct gcaataccat ctttgggtgg gaataaacga
     3901 aagaatccct ttgcaggatt agtctggaag tcatcagcct caatccaaag ctctcgacca
     3961 aaatgaaact cccgtttacc taattctgga tggtgcggat ggcgaggagc agagcacaat
     4021 tctttctgat cctcaggata gttctggatg attagtttga gcggcttgag taccgcagtt
     4081 gcgcgaggcg cacgtacatc caaatcatcc cgcagggctt gttccaggac gctcatatca
     4141 atccagctat cggctttaga gacgccaatg cgttcacaaa ataagcgaat actctctggg
     4201 gtatagccac gacgccgaat cccaacaatg gttggcatgc gaggatcatc ccaaccatcc
     4261 acccgttttt cttcaacaag ctgcaatagc ttgcgcttac tggtaatcgt gtaggtcaga
     4321 ttaagacggg caaattcgta ttgacgggga agcggaccct taaatacccc agcttctttc
     4381 aaggcgttta agacccaatc atagagaggg cgattgtttt caaactctag agtgcagata
     4441 gagtgcgaga cgttctcaag agcatcggag atgcagtggg tgaaatcata caaaggataa
     4501 atacaccatt gatccccagt gcgatgatga tgggcatggc gaatacggta gatcacagga
     4561 tcacgcatca caatattcgg atgcgtcata tcgattttga gacgcaatac atgactgcca
     4621 tccacaaact ctccagcacg cattttttgg aagagggcta gattttctga gggcgggcga
     4681 tctcgatacg ggctattttt accaggggta gcaaagtttc cacgattcat atgaatctca
     4741 tccgcacttt gactatcaac atatgcctta ccatgctcaa tcaatatctc tgcaaactta
     4801 tataagttct caaaataatc actggcgtaa tacaaatgct cttgaccccc atgattccag
     4861 tcaaagccca gccagcggac agcatccaaa atgctatcaa catactcaac atcctctttg
     4921 gtgggattcg tatcgtcaag acgcatatgg caccgtccan nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnaaataacc attgggctca ggagggaagc gcgtgatgac agcaggcaaa ggcttaccgg
     5101 cctgatccac tcggctgaga tatgttccct tagctaaatc atggtcaatg atctggcgta
     5161 gaaaattaga tggctcagaa ggctgattgg atccggtcga tttggcatta tcagaagaca
     5221 tcttgctatt gtagataaga ctggctgtaa tgacctaagt gctacccaaa ccgaagcctt
     5281 gccttaaaca ataatgcccg ctagtagcgg gcattattca aagcggtaga tattgccaat
     5341 tacagctctt cagtaaatac ctcttgtcga cttttcttga tggctggtag agccacaatc
     5401 accactaaaa aggctgctgc tagcagcaga cccaaagaca gtgggcgcgt gaggaaggtt
     5461 gtgaaatcgc ccctggataa gagtagagca cgacggaagt tttcttccat cattggacct
     5521 aatacgaatc ctaacaagag gggtgccggc tcgcaaccaa acttgaagaa catataacca
     5581 attacaccaa acccagcagt gataaagaca tcgaagatgg agttgttaac ggtatagacg
     5641 ccaatacaac agaacaccaa aattgctgga taaagtaagc gatacggaat cttcagtaac
     5701 ttcacccaaa taccaattaa aggcaaattc aagaggatga gcatgatgtt gccaatccac
     5761 attgaggcaa tcaggcccca gaacaacgca ggattgctgg tcattacttg cggacctggc
     5821 tgaatgttat gaatggtcat tgcacccacc atcaaagcca tcactgcatt tggcggaatg
     5881 ccgagggtca gcaatggaat aaatgaggtt tgagctgctg cattattagc gctttctgga
     5941 ccggcaacac cctcaattgc ccccttgccg aactcctccg agtgcttgga ggattttttc
     6001 tcaaccgtgt aggcgccaaa cgctgccagg gccgcgccac cgccaggcaa aattccaagg
     6061 atcgatccaa tggtagtgcc tcgcaaaata gaggggatca ttcgtttcac atctacccaa
     6121 gttggaataa gcgtagttac tttattgagg aaggactctc cagttccctt tttctcaagg
     6181 ttgagcatga tctcagcaaa gccaaataca cccatggcca ctgacacgaa accaattccg
     6241 tcggttagct gaggtatgtc aaaggagtac ctagcaacac ccgagttcac atcggtacca
     6301 acaagaccaa gtagcaggcc aagcaaaatc attccaatcg ctttgatgag ggagcctgat
     6361 gccagtacaa ccgcaccaat cagacccaat accatcaagg aaaaatattc tgcaggacca
     6421 aacttaaatg caacctctgc gagaggagct gcaaagccag ccaagatcaa tgtggcaacg
     6481 caacccgcaa agaatgaccc cataccagcg gtaaagaggg caacgccacc acgtccccgt
     6541 ttagccatct gataaccatc aattgcagtt acaaccgatg aggtctctcc aggaatatta
     6601 agtaagattg cggtagttga gcctccgtat tgggagccat agtaaatacc tgccaacata
     6661 atcagagcag cgatcggagg cagtgcgtag gttgccggca aaagcatggc aattgttgca
     6721 atgggaccaa gaccaggtag gacaccgatt aatgtgccta atacgcaacc gataaaacag
     6781 tacatcaggt tttgtagggt gaaagcggtt tcaaaaccca gagaaagatt atttagtaat
     6841 tccatttgat gatccgttct gcgggttaat tgagaaacgt tggaaataat ggaaattgca
     6901 gattcaagcc ctgaacaaat gcaaggtagg tgaaggtcac taaaaatagg gcattcaagg
     6961 cagtcccctt ccagctgaac tcatgacttg cgcttgccga tatgaagacc aagatgatga
     7021 ccgacaataa aaacccaagg gttggcaata gaatgccgta caaggcaact gatccagaaa
     7081 tccagatcat aatccgccaa tcccactttc caatcgactc ctcagtattt ttagaggaca
     7141 tggatttaat aagaatgtaa aaacccaagg cagtgagaat ggcgccaagc caaaaaggaa
     7201 agtagccagg ccccattttt gcagccgtac ccattttgta gttcagagca ttgagagcaa
     7261 agaaaaggcc aaaggccatg tacatgatcc cggcaccgaa atctttctta ttgcggatgt
     7321 tcaaaatcta gctccttatt tttgccaatt aggcacgact atatgtagta actgttttta
     7381 gtcgatttgt gattgtaagg gaatgatccg aggcaagctt aagggtttgc cctaattctg
     7441 tgccaatgcg ataattattg ggatgaaagt ctctgaaata cgccaagcct acctcgatta
     7501 tttcgcctcc aagggtcacc aaatcgtggc atccagcccc gttgttcccg gggatgaccc
     7561 cacccttttg tttaccaatg ccggcatgaa ccaattcaag gatgtctttc ttggctttga
     7621 caagcgccct tatcagcgag ccacgagctc ccaaaaatgc atccgcgccg gcggtaagca
     7681 caatgatcta gacaatgttg gttataccgc taggcaccat accttttttg agatgctggg
     7741 caatttttcg ttcggagatt atttcaagca agatgcgatt caatatgctt gggagttatt
     7801 aaccgaagtt tttaaactcc ctaaagataa attgtgggta acggtctacg ccgaggatga
     7861 tgaagcgtac gacatttggt tcaagcaaat cggagttccc aaagaacgaa ttgtgcgaat
     7921 tggggataac aaaggctcgc gttatgcatc ggataacttt tggatgatgg gcgatacagg
     7981 cccctgtggt ccttgtaccg aaattttcta tgaccatggc cccgagatcc ctggaggacc
     8041 tcccggaagc cctgatgagg atggagatcg tttcatcgag gtttggaata acgtcttcat
     8101 gcaatttaat cgtgatgaag cgggtgaaat gcatctttta ccaaaaccca gcgtagatac
     8161 gggtatgggt cttgagcgga ttgctgcggt cttgcagcat gttcactcta attatgagat
     8221 tgatctcttt attcatctct tgcaagcagc caagcaggca gtcgatgatg cgggggcaaa
     8281 aaattgtgac cctgatagcc cttcgctcaa agtgattgct gatcatattc gggcttgcag
     8341 ttttgttgta gttgacggtg tgattcccgg aaatgcgggt aggggttatg tgcttcgccg
     8401 tattgcacga cgtgcgatac gccacggtta taaattaggc gctcgtcaac ctttcttcca
     8461 taagcttgta cccgcattag tcgccgaaat gggtgatgct taccctgaat tgcgtcaagc
     8521 acaaactaag gtgatggatg ttctcaaaca agaggaagag cgcttcttcc aaacaatctc
     8581 taatgggatg gagatcttag aaggcgcact agcaaagggc gctaaggtct tggatggcga
     8641 tactgcattt cgtttacatg acacctttgg atttccattg gatttaaccg cagatgtttg
     8701 tcgggagcgc tcagtcagtg ttgactcgaa tggttttgag atcgcaatgc aaaagcaacg
     8761 cgatcaagct cgagccgcgg gtaagttcaa gatggcccaa ggccttgagt accatggaga
     8821 agctactcag tttcatggct atgacagttt gcaggtgcaa gatgcacggg tcactgcgct
     8881 ctatcgagat ggcagtcctg tagatcatat taaacctggc gagagcgcag tcgtggtcct
     8941 taatcaaacc cctttctatg cagaatccgg tgggcaagtt ggcgatcagg gtgagttacg
     9001 aagcgatcga gcgcaattta tcgtagccga tacctttaag attcaggccg atgtatttgg
     9061 tcaccagggc gagttacaag aaggtgaatt aaaggtcggc gatcttgttc aggcccgagt
     9121 cgatggcagc atccgtgtaa aaacggtacg taaccacagc gcaacccatc tcttgcataa
     9181 agccttgcgc gaggtcttgg gtgatcatgt acaacagaag ggctccctcg tcgatgctga
     9241 caaaactcgt tttgatttca cgcatactgc gccactctcg aaggcagaaa taaatcggat
     9301 cgagcaaatc gttaatcaag agattttggc aaataccgct agctcagcga ctgtgatggc
     9361 catcgaggat gcacaaaaaa caggtgcgat gatgctattt ggtgagaagt atggtgaacg
     9421 agttcgagta ttagaaattg gttcttctaa ggagttatgc ggtggcacac acgtccagcg
     9481 tacgggtgat attggtagtt ttaaaattat ttcagagagt ggcgttgcag ccggcattag
     9541 acggcttgag gcagtcactg gctccaacgt cctggatttt ctgcaaacct tagagtcccg
     9601 catcaatgaa gccgcgcaga ttctgaaagc ggctcctcat gagctagcac cccgtgtgac
     9661 acagttgcaa gacaatatcc gacaattgga acgtgaactg gagcgcgtga gctctaagct
     9721 agccgctagt caaggtgatg atctattaag taaagcgagc gatcatggtg ggctcaaagt
     9781 attagccgct cagttagata cagcagacgc caaagtatta cgcgagacta tggatacttt
     9841 aaaaacaaaa ctgaagtctg ctgtaatcgt tttggcatct gtgcaagatg gtaaggtaag
     9901 cttaattgct ggggtcacca gtgatgtaag taatcggatt aaggcaggtg atttggtgaa
     9961 ctttgttgct caacaggtag gtggcaaagg tggcggtaag cctgagatgg ccatggctgg
    10021 aggaacagat ccttctggtc ttgccaaggc cctagctggg gtcgatgcct gggtagccga
    10081 gcgagtctag gcgcaaatcg ctaaaataag aagatatgag gagaatgtaa tgagtgaagt
    10141 gagctccatc caagtccatc gcgaggttga tgccatcggc atgaactgcc ccctgcctat
    10201 tttacgtacg aagaagacct tagccgagat gcaatcaggc gaggttctta aactaaaggc
    10261 aactgattct ggtgctagcc atgacatccc ggccttctgt aaacaaacgg gtaacgagct
    10321 tctcgccacc gataatgagg gtgatgcgat gatcttttac gtaaagcgtc gttaattagt
    10381 accttacctt tgaagcattc tttgaactgc ttcgatcact tgatttggtt ttagtaaatc
    10441 aagacattcg ctcctactgt ctgatttgtc ttcacagccg gctttgcggc acggtacaca
    10501 atccccggga ccttgaagga tgcaaacgtt tttaactatt tgcatccgcg ttctaatttg
    10561 ataaggcttt tgtccaacaa aaccattagg ccatgggcca aagtttgttg ggggggttgc
    10621 cccaaataaa acaatcgtag gggtttcaca agcggctgat aaatgactga ccgatgtatc
    10681 aaccccgata taaagggtcg ctccttttaa gagagtgacc gcttgaggaa gacttaaatg
    10741 accttctgta ttaataaggg agccacgtat gtttatgtcg agtaaggaca aaatatcttg
    10801 gtttagttct aagtcctgcc gagctggcga agagctcaaa ataatctgat tgccatcatt
    10861 aactaaatgg gtgatcagtt cttgccaata cgatagcggc caacgtttat aggcattgag
    10921 aggccctggg tgcaaaacaa tgtagggttg ccgtatctgg ctttgaatga gaggagttaa
    10981 ggtttgtgct ttagggggtg ttactgagat tgggtgggca aataaatcaa gacccttgtc
    11041 ttggtagaag ggctccagta accttaattt ttctacaatg acatgttgat gaaagtaatc
    11101 cactggaacc cagtgcatag aaatggcgcg cttccaagca ctattttttt tacttcgggg
    11161 tgtgtcctca accaccacac ccactcgtcg taaggcagcc agcgaaccaa agaagtagga
    11221 gcgatcactt ggttgggtga tgatcgctag gtcatagcgc tggaagagtt gattaaagag
    11281 tttcagatac tcccataagt ttgggcgctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnc gatatcccag aaaatcaaat tgggcattgg gccaaatggc gcgagctgaa
    11401 ctaatcaatg gggttgttac taagacatct ccaatctgcc gagtggcaat gaataaaacg
    11461 cgggcagggt ctttcatagc ctattgaaat tgaccggctc gcataagccg gaccttttcg
    11521 agaattcgtt ttgtactttc atcgttattt gattgatctc gaactttatg aaatagatga
    11581 atgacttcag ttgaccatga gccagacttc cttatgattc ctttattttc aaggcgaaaa
    11641 atgaaatcag catcttcgtg accccatcca attagtgttt catcaaatcc tttaatggcc
    11701 agggcgtcct ctttccagca cgccatattg catcctttaa tgcgacgcca gaagaatttt
    11761 ttgtaatttc tccatttacc gttaccaaat ttaatgaata aaggaagaaa tttatttata
    11821 gacccttgaa tacgtaaaaa gaaggaattt ttttgtaatc tggagaagtc tatatatcct
    11881 tcagtgagta tttcttgggt aaatttctgg ttaagcaata ttctgcttcc tgtgactaaa
    11941 tacccctttt gtgccagaga taaatgtctc tgaagatagt cgggctgaac tacgcagtct
    12001 ccatctaaaa aaattaagta ctgaccattc gcactattaa tagcgcgatt tccgatgatt
    12061 gtcttgcggc aacctatatc ttcatgccaa agatgcgtaa ttttgatggg gaattcttga
    12121 atatactttt gaatgagatt acgagtttct tcttttgacc catcatcagc aatcaaaatc
    12181 tcaaaatctc taaaggattg attctttaag gattgaagac aaagacccaa ggccgctggc
    12241 caattgtagg tcgccactat gacagaaatt tgagtcattc ttttgatttc ttgattagac
    12301 gccaaagttt aatgtacctt aggtaggagc cttttgcatt ggctatagcc aatgaaaatc
    12361 cttggggacc atctaaaaat ccagctcgta ttacataagt acgaataaat gaccaagtgc
    12421 catgcaatat agccttaaga ggacttgctg ttttaccctt agcaaatgct tgttgtgcag
    12481 atgcggtgga atatcgctgc attttttctt gtacctgagc ttggttcata aagctatagt
    12541 gaagcatctg attctttaac ttcattacag aatcatgcgg gattagacgc tcatgaacta
    12601 aatcgtttga gaagcgtgcg ctacctctct taaatagtcg gtctacataa tccggggtcc
    12661 aacccgagtg acgcataaag cgcccacaat accaagatag tcttggaatg gcatagcaat
    12721 tcgcttgggg tttctcaagc acacccttaa tctccagcct gagctcgggg gtgaggcgct
    12781 catcggcatc tagggagagc acccagtcac cggtggcaag ggctaaagcc cgattctttt
    12841 gaggcccaaa tcctggccaa tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnngt atccacgacc acaatctggt cggcaagccc gtctagcgag
    12961 gctaggcagt cctcgagatt agcttcttca ttccgagtta tgagtatggc tgataaggtg
    13021 ggcataattc attatatgaa tgcttcagac cgtcaagccc taaaccgatt aattgggtat
    13081 ctacgccccc atatccgcct catcattggg tccttactcg ctatggcgat agttgcagcc
    13141 tcagagacct ctatcccggc tttaatgaag cctcttttgg atcgtggctt tactggagag
    13201 atgaacgata agctctggct ggtcccggta tttttagttg gcttggcttt tactcggagt
    13261 ggcgcacagt ttctgtctaa ctatctactg accaaggtta ttagtaatat tttgcttaaa
    13321 ttacgggagc agatgttcac tcggctatta cgagcaaaga ccgagtacta ccagaagact
    13381 tcagcgtcta acttgattaa tgcggttgtg tttgaggtaa ataacgtcct ctccatcatg
    13441 ggtggcatgc tcattagtct tgtaagagat ttattgaccg tcattggttt gatgggttat
    13501 ttgttttatc tcaattggcg tcttacttta gtggtattaa ttatttttcc gatcattgcc
    13561 tttgtgatga gcaagatcaa taaacgctta cgcggcttaa atcgtca
//
LOCUS       DDPQ01000021            2513 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_33619,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000021 DDPQ01000000
VERSION     DDPQ01000021.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_33619"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    138..247
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          594 a          523 c          549 g          737 t
ORIGIN      
        1 tcacccaaga catggatatc ctttgccgca gtggactcaa aattaatgta attgacattc
       61 acccaacgtg cattggagtt ggctaatcca ctttgcactg caatgttgcc agctcgcatc
      121 atgggtagga tattcaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnggg tacattgatg cccaggcttt tttgaacaag ccgcctttgg aggtaacgtc
      301 atcattagca tccaaaatca gaactttgga tttaggcttg aactgcttga agtaactagc
      361 aacttggcag gcgcgctcat aaggcccggg tgggcagcga tagggggcag tgggaacact
      421 gattgcatat acaccaccgt ctttcatact ttcgagctgt ttacgtagca agacggtctc
      481 tggacctgct ttccatgcct gcacggttac accttcctgg tttgcctttt gtagaccttc
      541 aactcggtcc atcatcatgt caataccggg ggagagtact agcttgtcgt atttgatgga
      601 agcaccagaa gcaaggcgaa ccgttttctg tttcgcatca atcgatgtga ccatgtcctt
      661 tacaacctta ataccatgac gctttgtgag attgtcgtac ggggtggaga tattcgagat
      721 ggatgtgtgt ccgccaacaa ctaagttaga cattgggcaa gagatgaact cagcattggg
      781 ctcaatcaga gtaacctgag cgctgttatt agagaacatc cgcacatatt tagcagctgt
      841 tgcgccacca tatccaccgc caactaccac cacattggct ttactgatat tgattggatt
      901 tgcagcacat cctgctaaaa gacctaaaga ggcaccggag ctaacaccta aaaagtgacg
      961 acgattcatg atggttttcc tattattttt taccaaggat attggagatg gtttggagtt
     1021 gctcgtctgt ataacctttt gctaattgat gcatgatggt gccagtacga gcgccgctct
     1081 taaaggcttt taattgggtc agcattgctt cactgctgag gtgattaatt tggggcatgc
     1141 cagcattcgg aacgcccacc ccgtttgttc catggcagtt tgcacaggtt gcggctagac
     1201 ccatttgatt gatattgctt gctgttgact ggctcatagc aggagtactc actgcaagta
     1261 agcctgcggc actaatacct aaccctaatt tacttaaggt gcgcgctgat tgaaagaaca
     1321 tagtaaatat ctccattggt tttatttcga acaacacaac taaagcaaga aactatctta
     1381 aaggatggct cacaacttct tgagcaaatt tctattattt tctatacatt aattccataa
     1441 tcatataact caaggcgcat atacagatta aggttttccc taggttttaa aatatattga
     1501 tctgaattca ttggggatta accctaggct aataaactag agggtatgca aacaccgatt
     1561 tcctatcaat ctctcttttt atgcctagtg tttgcaatcg gagcgtactt ttatggcatc
     1621 gaaagccgct ttgctccaaa gaatggcgat gagtatccct atacccatat tgttcgcatg
     1681 actaatgcat ctggacattg gctaccgttg cagtcggaga tggagggtat taagaacact
     1741 aagccgccat tactattttg gcaaggaatg ctttcttcac agcgcgggca gagttggtct
     1801 ctgattgatt tacgttggcc aagtttgctt tacaccgcat tgacagccct ccttctcgct
     1861 ttggcagcta gcaaggtaac aaaaaattta tcgctcggta ttctggcagg tctaatctgg
     1921 ttagccttct tcaataccta tcgctatggc agaccgtatt tggcggagcc ccccgaaatt
     1981 ttttggctaa gtctaccgtt ctttgggttg ctcctattcg gtaagcgtgc atttgagtcg
     2041 aagtggattt ttccattact gaccggcatt tctttagggt tagcgctttt atacaaatcg
     2101 attgcctatg ttgtgccagt ttgcttggtc ttggttgctt ggtattggca atggcgctct
     2161 tatcgaattc ttgagttgct gggtaaagat gctgtcaagg tgatcttcgt gggcgcgatg
     2221 tcaatcgcat gcttttgcct ctggtttgta ttggatcctg atccgatggc aatctggaac
     2281 gagttcattc tgggtgagaa tgccggcaag tttgcggccc gcaatagtaa ctacctaaaa
     2341 gatatgctgt ggggtggtga tagcatcggc atgctttttc tcagcagcat tgcgaatgca
     2401 ggctttttat cgctcttggt actcaatttg ttttaccttg caattcgtca gtacagacaa
     2461 actactgttg agcaaaaatt actttggatc tggattgctg tatttttctt aat
//
LOCUS       DDPQ01000022           21512 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_4702,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000022 DDPQ01000000
VERSION     DDPQ01000022.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21512)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_4702"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    4235..4254
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5835..5914
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7734..7764
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18546..18634
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5627 a         4339 c         5507 g         5810 t
ORIGIN      
        1 ctgagtgctg gcggacaaga attaccagtc ccaattaaga cagccatggc aggtatttca
       61 aaaataatga catcaactgc ctataaaatc taatttactg ttcgtttata cagtcttttc
      121 agtgagtaat cgctatatac ctaaagacgt atcttaggta taagttacaa gtcattgttt
      181 tacagcataa ttttacttaa accttttctt gcaaagattc tctaagtgat tgtaatcact
      241 aaggaatttc ctaaaaattt acccaaaaat acttgaccct atcagaagct tcctctaaag
      301 tgggaggaag tgtgaaaaag tggggtaaat tttgtttcaa ggtgcgtcag ctcttaatct
      361 cgatgcgaaa ggacgtatgt cggtgccgac aaggcatcgt gacgccctta tggtgcaagg
      421 agagggccgc gtcaccttaa caaaacatcc tgatggatgt ttattgttat ttccacgacc
      481 cgagtgggag gcttttcgta cccgtattgc tcaattgccg atggatgcac attggtggcg
      541 cagaatattt ttaggtaatg ccgcagagat tgatctggat agcgccggca gaattttagt
      601 aagcccagag ttgcgcagtg cggcaggcct tgatcgcgaa atcatgttgt taggcatggg
      661 atcccatcta gaaatctggg atgcaaatac atacgccagc aaagagcagg cagccattgc
      721 ccaaggcatg cctgatgcac tcaagcaatt taccttttaa tgattgggcg acatgaacaa
      781 tacccatcgc ccagtattac tggccgaggc agtcaccgcc atgatcagca gttcatcatt
      841 tcagaaggaa aacgctctta tcgtcgacgg aacctttggt cgaggcggac acagcaaagc
      901 cttattagaa caaatgggat ccaattgcaa actcattgcc ttcgacaagg atttagaggc
      961 aataagcaca gcccaaaaaa taatagatcc acgtttacaa ataattcaca gcagttttgc
     1021 aatgatggat cagtatgtca aatcacaaac tgtggatgcc gtattactcg atctaggaat
     1081 cagttcgcca caaatcgata cgccggagcg aggattttca tttcgcttcg atgggccgct
     1141 cgacatgcga atggatacca gttgtggggt atctgcagca cagtggcttg caagctctcc
     1201 ggtcgaggag attacgcaag tgattaaaac atttggcgag gagcgttttg cattcgcgat
     1261 tgctcgcgca atcgtaaagc agcgcgaaga aggcaaagcg gtcgaaacga cactagagct
     1321 atcaaattta attgccaaaa cggtacgtac ccgagagcca ggtcaagatc cagccacacg
     1381 tacctttcaa gcaattcgta tttttataaa tcaagaatta gctgatttag agagagggct
     1441 ggaagctacc ctcgatgttt taaaaatagg cggtcgcttg gtggttatca gctttcattc
     1501 tttagaagac cgcattgtga agcagtttat gcaatcgcat tcgcaaatca cagtacctcg
     1561 cgagttgccc attcgggcca aagatttgcc ccagggagat cttgagctta ttgccagaat
     1621 caagcctagt gtgtcagaga ttgaggagaa tccacgcgca cgttcagcag tcatgcgaat
     1681 tgctgaaaaa agagggatgg cgacatgaat cgcgcaaccc ttacattact tgcacttctc
     1741 ttaatctgcg ccctgtcatt ggttgcttca caacagcggg ctagaaaaac atttgtagct
     1801 ttagagcgcg cacaagtaca agaacgtaaa ttaaatcaag agtggttacg acttgagttt
     1861 gagcaaagaa atttatccaa gtcatcacga attcgggatg tggcacgtaa tcaattgcgc
     1921 atgagcccca ttactcctga tcgaactctc tactttaagg aaggtctatg aagccagtca
     1981 ggttctcgac ctcacctaat gttgtcttgc gcttaccgat gtggcgctca agactcatgc
     2041 tcttcctact gtttgtagca tttgctgcat tgggcatccg tgctttttgg atacagggac
     2101 ccggtaatgg attttatgaa gctaaagcta ttaaaggggt gcagcgtgag attgacatgc
     2161 ccgcaactcg tggcaagatc ttggatcgta atgggcaatt aattgctacc agcttagagg
     2221 caaaagcagt aattgcctat accgataaca tccctgcgga ccttgcacca gaaaagatta
     2281 cccaactcgc caaattatta gagatgagcg agactgaaat caagaaaaaa tttaaggatg
     2341 aacgcaatca agttttttta aaaagacaag ttgacccaga aatcgctcag caggttaggc
     2401 gccttgaaat accgggcatt ggattaaata atgaataccg tcgtcactat ccagagggtg
     2461 aggcgatggc gcacgtcatt ggatttacaa atgtggagga tcgtgggcaa gaaggaattg
     2521 agcttgcgcg tgataaggac cttgcagccc aagctggaaa acgtcgtgta gtggtagatc
     2581 gattaggccg tattgtgaat gatattgcca tattacagtt tccacaaaac ggccaagatc
     2641 ttcagctctc catcgatagc aaagttcaat atattgccta taacgcttta aagagtgcgg
     2701 tagaaaaaca taaagcaaaa gcaggtggcg cagttgtttt agacgttcgt tctggtgaaa
     2761 ttctggcatt agcaaattgg ccaagctata acccaaactc tcgtaaatca ctaacgggtg
     2821 agcagttacg taatcgagta cttacggata cgtttgagcc tggctcaacc atcaaaccat
     2881 ttccagtggc tttggtaatg gagcagggca aagttcagcc agatactaat atgacaattg
     2941 gtcaaaagtt actaattgga cctaagccaa ttacggatac ccatcctcat gggatgttaa
     3001 cggtctcaca ggttattcag aaatctagca atattggtac agcaaaatta gccttacaac
     3061 tcgaaccgag agagatgtgg gatctatttg cagcagtagg ttttggacag gcacccaaca
     3121 ttggttttcc aggcgcagtt gcgggacgct tacatccaca tcaaaaatgg ggtcaaagtg
     3181 atcaggcccg tatgtctttt ggttatggaa tctcgacatc tttattccag ttggcacgcg
     3241 cttacaccat ctttgcacgc gatggtgagt tggtaccaac cacgatcctt cgtaacccag
     3301 aatacaaacc agggacacga gttatgtccg caaagacagc aatcgagatg cgcaatatgc
     3361 tggaaacagt taccgagcag gggggtacag caatacgcgc acgtgcagat ggatttaggg
     3421 ttggaggaaa aacaggcaca gcgcataaag ttgaaagcaa gggatatgtc gcaaataaat
     3481 accgtgcatt ttttgtgggc cttgcaccca tcagcgcacc acgtattgtt gttgcagtta
     3541 tggtcgacga gccttcagct ggagggcatt atggcggcga ggtcgccgcc ccagtttttt
     3601 caaccatcgt gagtgaaacg ctacgtagcc taaatatttc accagatctt accgatcgta
     3661 ccttagttca aaattttgag ccaattccaa ccggcgtgca ggcacaaaaa gtggtgctca
     3721 aacaatgatg gctactataa aaatgaaccg ccatgaagct ctattgcatt tacaacaagt
     3781 agtggagccg actgcaagga tatctgctga cagtcgacaa ataaaagtag gcgatatctt
     3841 tatggcctat gcagttgggc atggtaaagc cttgcgnnnn nnnnngctgg gaatcagatc
     3901 tagatgatga gcgttgtatt tgtgttgaaa atcttgtaga tgatgctggc tggatcgctg
     3961 cagaatggta tggcagtccc agccaaacac ttaagctaat tggggtaacc ggtacgaatg
     4021 gaaaaaccag tgttacacaa tggcttgctc agactttatc caagcatgca gcagtgattg
     4081 gcacattagg aattggcttt agcgatcaat tacacgatgt gggctacacc acacccgatg
     4141 cgccgcgctt acaaacagag ttatttaagt tgaagcagct aggcgctcaa tatgtggccc
     4201 tagaggtgtc atcacacgct ttggaccaag ggcgnnnnnn nnnnnnnnnn nnnncgaagc
     4261 ttgttcatgg atcttatgaa tcgtgatgac aagatgattt ggctttatgc attgaatcca
     4321 gcagcattcc ataatttaga aaaacttcaa gatcgtttta agctagttta tttgcaagag
     4381 ttttgtttta tagaaaatgc ctatcaatgc aagattgtga ttgacggaaa agtgtatcca
     4441 tgcacgattc ctgtattggg tgagttcaat ctagaaaatg ctttagcggt tattgctacc
     4501 ttaatcgcag aaggtattga tattcctgaa gcgttacggc aagtcgagtg tttgaggccc
     4561 gtaaggggac gcatggaaat cataaaaggg gcaaaggcag acgccccaat gatggttgtg
     4621 gactatgcgc atacacccga tgcactccaa aaagttcttg aaacacttag gccaattgcc
     4681 gcaattcggc agggacagat ttattgtgtc tttggttgtg gtggagatcg cgatcgtgaa
     4741 aagcgtccga tgatgggaca aattgctcga gagggctctg atcacttggt tataaccagc
     4801 gacaatccgc gctcggagga tcctttgcaa attatgcaga tgatctttgc tggtgtcagg
     4861 caagtaacaa atagaaaaga agattctaaa acggtcgaga tgattgcaga tcgtgccgcc
     4921 gccattatga gtgcagtgcg acaagccagc ccacaagata ttgttttagt tgctggtaag
     4981 ggacatgaga ccactcaaga ggtgaatgga aaacgatttg atttttctga tcaagcccat
     5041 ctacgtttag ccagtggagg ggcggtgtaa tgcatgcaat gaatccgatg accaatttag
     5101 cgcagataca ggcgatgctg cccaacagcc atttcatcaa tattgcgaaa gaacaggcca
     5161 agggacttag cattacaaaa attggtacgg atagtcgtaa gttaatagct ggtgagctat
     5221 ttatagcgct tcagggtgag cgatttgacg ctcatcactt tctggatgac gttcagaagc
     5281 aaggggccgc cgccgtaatt attagtgatg ccgccgcatg tccaaaatcg atgccagcag
     5341 tttatgtcac cgatactcgt aaaagcttgg ctgagctagc tcacgcttgg cgtaaacaat
     5401 atgagattcc cctcgcagtg gttactggta gtaacggtaa aaccactgtt aaagaaatga
     5461 ttgcttctat atttaaggaa gcagttggct caactcaaac cttaatgact caagggaatc
     5521 ttaacaatga gattggctta cctctcaccc tcttgcaatt gagatcagag catcgtttgg
     5581 cggtgattga gttaggtatg aatcatcctg gagaaactgc agaattagct gcaattgcca
     5641 aaggaaatat tgctcttatt aataatgcac aacgcgagca tcaggagttt atgcaaagta
     5701 ttgatgcagt cgccaatgaa catgctgcgg taatcctagc cctaccgcac gatggtatag
     5761 cagtatttcc agcggagacc ccttacacag cgctttggag agaggctgct ggttcgcgta
     5821 aacagattga ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncataat tgtgcgaacg cattagcagc
     5941 ctgtgcggta gctcttgctg ccggaatttc gttagacacg attcggcttg gcttagaaaa
     6001 atttatgcca gtctcaggca gaatgcaagt acagcggtta tcagctcaca cacgtttaat
     6061 taatgacacg tataacgcaa atccagattc ggtagtagct gccattaatg ctttggcgca
     6121 gcatacgggt gagcgctatt tagttttagg ggatatgggt gaggtaggta cccaagggcc
     6181 agagttccat gaggagctcg ggcgctatgc cgcaaaatgt ggcattgagc atctattaac
     6241 gctcggtgac ttaagtcaaa tttccaccgc agcttattta aaagcaaagc caaatggcca
     6301 ggccaagcat tactctgagc taagccaatt aaatcaagat ttacaaagtc agattagtcg
     6361 agagagcctt gaacatacca gagtaattac tgtgttagta aaaggttcac gctttatgcg
     6421 catggagcga gttgtagagg cactccagca ggagatcaaa gcatgctctt aatcttggca
     6481 caatggttac aggacgacta cggatttttt cgggtcttct cctacattac atttcgagcg
     6541 gtaatggcaa ccttaacagc cctattgatt ggtcttgttt ttggtccttg ggtgattcga
     6601 aaacttaccg ccttaaaagc agaccaagca atacgaacgg atggccctca aacacattta
     6661 gcaaaagtcg ggacgccaac gatgggcggc atcttgattt tgatcggaat attcatctca
     6721 tgtgttttgt gggccgacct cagtaatcgc tttgtttgga ttgtgatgat tgtgaccttt
     6781 ggatttggtg ccattggttg ggttgatgat taccgaaagg tcgtccacaa aaacccaaag
     6841 ggtatggcat ctcgagaaaa gttcctttgg caaaccctta taggtttatt tgctgcgatt
     6901 tacttagcct tttctgtatc cgaggtcaac aactataaag tcttacaact ttttatgagt
     6961 tgggtcgaga gtggcttctc attggatttg cctgcaaagt caaatttata tgtccccttc
     7021 atgaaagaag tgagctatcc attgggagtg atgggtttca tcatcctgag ctatctcgta
     7081 atagtaggta gcagtaatgc ggtcaactta accgatggtc ttgatggcct tgtcatcatg
     7141 ccagtgatct tggtaggtgc tgcgcttggc gcattcgctt atgtcatggg taactccatt
     7201 tatgccaaat acctactttt cccatatatc ccgggggctg gcgagttatt aattttctgt
     7261 ggcgcaatgg gtggcgcagg tcttgcattt ctttggtaca acgcgcatcc cgctcaagta
     7321 tttatgggag atgttggtgc attggcccta gggggcgcgc tgggaacgat tgccgtgatt
     7381 gttcgacaag aaattgtgct ctttgtgatg ggaggcatct tcgttgccga gaccgtctcg
     7441 gtcatgatgc aggttctttg gttcaagttc acaaaaaaat attacggaca ggggcgacgg
     7501 atatttcgga tggcaccact tcatcatcat tttgaactag gcggttggcg tgagacccaa
     7561 gtggttgtgc ggttttggat tatcaccatt ctgttggtat tagttggctt atcaagtttg
     7621 aaattacgtt aggtatttat taataaacaa tgaaacaggt attacagaat ccctttttag
     7681 gacaagccgg tgctgatgca tccgagcgat ctgcacccca acgtttcttg attnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnntgttcg tttagtagat acccgtgctc attctgacct
     7801 gcgttcgcaa caactggagt ggttggctga tttacaaaaa ttaggattac aagacgcatg
     7861 ctttgaatca agtgattgga atactttatt agatggggtg gatacagtcg gtattagccc
     7921 tggcttgtcc ccgatccaag agccaacagc agagcttttg catctatgtc aagaacgtaa
     7981 tatttcagtg tggagtgaga tcgagttctt tgcaagagca atccttgctt tgcaaactat
     8041 gtccatacct gccgaagata tatacaagcc aagtattctt gcgattactg gcacgaatgg
     8101 taagacaacg acttgcgcac tgaccgccca gatctgtcag cgtgcaggta agcgcgttgc
     8161 tcttgcagga aatattagtc ccgccgcact tgagaaattg ctcagcgtac ttgctgaggc
     8221 taatagctat tcagagctgc cagagatctg ggtgcttgag ttatctagct tccaattgca
     8281 atttacacat accttggatc caaccgcagc tacggttctc aatttatcgc aagatcatct
     8341 cgattggcat ggagatatgc aagcctatgc agcagccaag gctcgaattt tgggaaagaa
     8401 ttctgtattg gtacttaacc gagatgatga agttgtgaca gcattatttg ccgatgagaa
     8461 agaaagtcgg aaggttatcc aatttggtag cgatggtcct gcagatgtag attcttttgg
     8521 tattgagaga gatcctagag cgggtgggat cgattggctt gtttgggcag tgccagatga
     8581 ggccacttta gcaagcgagc atgaagaggc acaagaaggc tttattaaac gcaaacgtcg
     8641 taagaaatca gacgtctatg aaaagtccga tgaacttcgg atcaaacgtt taatcccagc
     8701 tgatgcattg cgtattcgag ggcgtcacaa tgcactcaat gcgctcgccg cactggcctt
     8761 agcgcgggct gccggcttag gaattagcct tctattgcat ggcctacgcg agtatcaagg
     8821 tgaaccccat cgcgttcaga cagtagctgt gatcgcagat gttgagtaca tcgacgatag
     8881 caaaggtacc aatgtggggg ctacagttgc tgctttacat gggctaggat cagctacagg
     8941 aagtaaaaat atttggttaa ttgctggagg tgatggcaag ggacaagact tttcaccctt
     9001 agttgaacca attacacgat tcgcaaaagg tgttgtatta attgggcgcg acggtcctgc
     9061 tatcgaagca gtgtgtgctc aggccataga agatcatgcg ttgcaactaa tacgcgcaaa
     9121 tagtttagag tctgccgtcg agcaaacctt tctccaagca aaagccgggg atattgtttt
     9181 attgtcgcca gcatgtgcaa gttttgatat gtttaaagac tataagcatc gggctgatgt
     9241 ttttgagcag gcggttcatg agttagcgca tcaaatttca tcaagcttga atgatccagt
     9301 gagggcgtca gtatgaacac tcgcccaaac cgtttttgga atatctcgcg agaaggtttc
     9361 gatggctttc gtagtggtct acgagatgct gtatcaggcg ttgagcaaac acgttcccgc
     9421 atgatggagt atgaccagtt attagtctgg gctattttgt ccttagcctt aattggcttg
     9481 gtaatggtgt attcagcctc gattacttta gccgatggcc ctaaatacgc caattacagt
     9541 agtaatcatt ttttagtacg gcacttaatt tctttaggga ttgcaacatt tgttggattt
     9601 tgggttttta agattccagt caaggcgtgg gataagttgg ctccttactt attcgctttc
     9661 accattctgt tattaattgc tgttctgata cctggaatcg gtaaaggtgt gaatggagcc
     9721 cgtcgttgga tccccttggg gcttatgaat tttcagcctt ctgagctaat gaaattcgcg
     9781 gtcattattt ttgccgcaag ttataccgtt caaagacaag agcatttgca gtcttttgtt
     9841 aagggttttg tgccgatggg cttagcagtt gcattagtcg gcctgttatt aatgttagag
     9901 cctgatatgg gcgcccttat ggttattgga ataatcgcca tgggtatttt gttcttaggc
     9961 ggcattaacg ctaagttatt cggtggcttg acgctcgtag gaattttgat tgtggcagcc
    10021 gtgattactt tatcggagtt tcgacggcaa cgtatctttg ccttcctgga tccctggcag
    10081 gctgagcatg cagcacgcaa gggatatcag ctagttcatt ccttaatggc ctttgggcgg
    10141 ggtgagtggt ttggagttgg tttgggtggc agcgttgaaa aactgcatta cttgcccgag
    10201 gctcatactg attttatctt ggccgtcatt ggtgaagagc ttggatttgt gggcgtctgt
    10261 gttgtgatct tgctctttta ttggatagta cgtcgagcat tcttaattgg tcgaacagcc
    10321 ttgcagttag atcgtagctt cgctggttta tgtgccaaag gaattgcaat ctggattggt
    10381 tggcaagcct ttatcaatat gggcgttaat ttaggtttgt tgccaacaaa aggcctaacc
    10441 ttgccgctag taagttatgg cggatctgcc attttgatga atgcagttgc attcgcagtg
    10501 ttactcaaga ttgattacga aaatcggata ttgatgcggg ggggtaagtt atgagttcaa
    10561 acccgcgctc gatcttggtc atggctggcg gaacgggagg gcatatcttc cctggcttag
    10621 ccgttgccga aaatcttcgt ggtcagggct gggatgtgtc ttggttaggt aatgccagtg
    10681 gaatggagta tcgcttagtg ccagccaagg gttttcagtt tgaaagcatt cagtttggcg
    10741 gactaagagg aaaaggcctc ctgactaaag tgatgttgcc atttaatttg ctgcgcgcca
    10801 tggcacaaag ttggcaaatt attcggagac taaaacccag tgttgtgttg ggaatgggcg
    10861 gatacattac cttcccaggt ggtctggtaa gttatctttt gggccgccct cttgttttgc
    10921 atgagtctaa ctccgtggca ggaagtgcta atcggttttt agcaaaagtg gctgctcgtg
    10981 ttttgacagg atttccgaaa acgatgccca atgcagaatg ggttggtaat cctattcgag
    11041 aaggttttga gacccttacc gcttgcgatg aacgttatgc agcacggcaa ggacctcttc
    11101 gcttattggt tgttgggggt agtcttgggg cggcggtctt aaatacgatc attccggagg
    11161 ctttatctca tataccgatc gagcaacgtc ctatggtgct ccatcaggcg ggtgagaagc
    11221 atgcgaatga actggtgaag cactatcgtg atttgcatgt aagcgccgaa gtaaaaccat
    11281 ttattgagga tatggcatct gcttatgcaa atgctgatct agtaatttgt cgagccggtg
    11341 ccatgactgt atccgaaatc tcaagcgcag gagtagctgc ttgttttgta ccattcccgt
    11401 atgcaattga tgatcatcaa actgcaaacg cacgattctt atcagatgca gaagcagcca
    11461 ttttgttacc acaggatcag ctatctgcgc agtctttagc caagttattg cagaacatca
    11521 atcgggatga gttatctgcg atggcacaaa aagcacaaga gcgtgctaag caccatgcta
    11581 cggagcgggt tgcaagtatt tgtgcggagt gtgccaaatg aagcacattg ttcagcacat
    11641 tcattttgtg ggcattggtg gtgccggcat gagcggtatt gctgaggttc tccttaattt
    11701 aggttatcgc gtctctggat ctgatctatc tgcatcagca actacaaagc gtttggttga
    11761 gctcggcgca agcattcaga ttgggcatga tgcaaagcat attggtagtg ctgaagctgt
    11821 ggtgatctca agcgccgttg ctggaaataa tcctgaagtg ttagcagcaa gagcagctca
    11881 tattcctgta attcagcggg cggtcatgct tggtgagtta atgcgtttaa agcaggggat
    11941 tgcaattgcg ggtacgcatg gcaagacaac tacaaccagc ttggtcgcat ctgttttagc
    12001 gcaaggcggg ctagatccca cttttgtgat tggcgggaag ctcaattcag caggggtcaa
    12061 tgcacgctta ggacagggtg actttattgt ggtcgaagcg gatgagtccg atgcatcatt
    12121 cttgcaatta tttccagcca tggaggtgat cacaaatatt gatgcggatc acatggatac
    12181 ctatcagcac gatatggctc gtcttaagca ggcatttgtg caatttaccc agcgtatgcc
    12241 tttttatggg attgctgttt tatgtattga tgacgctaac gtgagagata ttgtcccctt
    12301 tgtctcacag cccatcttgc ggtatggctt ctctgatgat gccgatatcc gcgcgagcga
    12361 cattgaggca attggtacac aaatgcaatt tactgttcat cgcaggacag ttcggcgtca
    12421 tggtacacag cctggcccac ttaagatacg gcttaacttg cccggtaagc acaacgttct
    12481 aaatgcgctt gctgcaatag ggattgcaac agaactgggc gttagtgacg agtcgattat
    12541 gaaagccttg gcacaattta gtggcgtggg gagacgtttt cagcatcacg gcgatatcaa
    12601 attagagggt gggcgccaat ttactctaat tgatgattat ggccatcacc cggtagagat
    12661 gaatgcaact cttgcggcag cccgcggagc atatcctaag cgtcgtttgg tcttggcatt
    12721 tcagccacat cgttttactc gtacacgaga ttgttttgga gattttgtgc aggtattacg
    12781 tcaatttgat gcagtggttc tcaccaatgt ctatcctgcc ggtgaagccc gtatcacggg
    12841 ggctgatagt aaggcactca ttaaggccct tggaaacaaa gtgccgaccc aattggttga
    12901 ggaagtgaaa gaaatgcccg cggccttaag tgcactttta caagatggcg acgtattaat
    12961 tacgatgggc gccggctcga tttctcagtt accccatgcg attgtggaga tgaagtatgt
    13021 ctagatctca atcacgctgg agccaacaag tagccatgga tctggctgct atagatccca
    13081 aacaattagg acgtgttgcg gttctcatgg gaggacgctc agcagagcgc gagatatcac
    13141 ttctatccgg caatggggtc ttaaaggctt tactagagaa gggtatcgat gcgcactcat
    13201 ttgatccagg cttgcggcat cctgcagaaa ttgcagcaga gaattttgat cgagcgttca
    13261 ttaccttgca tggtcgttat ggtgaagatg gcactattca ggggctttta gagctatttg
    13321 gtattcctta taccggtagc ggtgtattgg catcagcgct cgctatcgat aaggttgcta
    13381 ctaaacgcat ttggattagc aatcaattac aaaatcctcg ctttgaggta ttaacagaac
    13441 aaagtaattg gtcgcaagtt gttcataaac ttggattacc aatcatcgtc aagccggccc
    13501 atgagggctc ttcattggga ctcagtaaag tagcgcatgt tgacgatttg cctaaggcct
    13561 atgcattagc agctcgttta gatagatgcg tactcgcaga gcaatgcatt attgggcctg
    13621 agttaacttg tccaattgta ggcgagggcg ataccgctca ggccctaccg gttattcgga
    13681 tcatgccgcc accagcagga tatgactttc atcataaata tttctccgat gaaacaaaat
    13741 atttatgtcc aaccgactta gatcccagga ttgaggcgcg ggttcaagaa ttagccttgg
    13801 catcctaccg tgctttaggt tgtcgtactt ggggtagggc tgatgtgatg ctggatgaag
    13861 cacatcaact ggagccctat ctcatagaaa tgaatacttc tccaggtatg acatcgcact
    13921 ccttggtacc gatgtccgca atggcggcag gagtttccta tgccgattta gtactttgga
    13981 ttttgatcca gactctaaag agtgttcctg aaaagattaa aggagcgagc tcatgagtac
    14041 cttgatgaat cggctctctg agttatgcgc aatggcagca gggcctgttt ggaactaccc
    14101 agagcggatg gctgaaatta gtcgttggct aatgcgcttc tttgttctgg ttgttctggt
    14161 atcggtcttg ctgtggatta gtcagcagcc agtatttacc ttacgtcatg tacagattga
    14221 gtctgcaggc gataaagatt tgcagcatgt ccaattagca atggttcgca gccaagtatt
    14281 ggataaggta caaggaaact tttttagtgt gcatttgcag gatgttaaga aagcctttga
    14341 agctttgcca tgggtcagac aagccagcgt acgtcgcgct tggcccaatg gtttagtggt
    14401 cagtattgag gagcaaaagc cattagcggt ttggggtaaa gcagagcaac aaaaattaat
    14461 gaatgtgtac ggtgaggtat ttgctggatc gatcaccgag cttgatgaga acacttcttt
    14521 ggttgagctc agtggaccag atggctctag taaagaggtc ttacggcttt accaaaagtc
    14581 aagcgcatgg tttaagcctt gggatgcaga agtgaaatcg ttagctctct cggatcgtta
    14641 tgcctggcat atcaagttga gtaacggcgt tcgaattgag tttggtcgcg aggaagagca
    14701 ggccaaagga gtattggagc agcgggttgc tcagttaatt caatattggc cgcaggtaca
    14761 gcaaaagtgg ccaagtcggg tcgatgcggt tgatttgcga tatccgaatg gatttgcagt
    14821 acgcattgtt ggcgcacctg ttaagactat tccgcccagt atcaaggtag aaaaccgtac
    14881 agagcaatta attgcttttg tgaaccacga tgatcaacgc agtgaggaat tgaaatgagc
    14941 aaagacaacc gagacttatt ggttggttta gatattggta catccaaggt ggtggccttg
    15001 gttgctgagt tgagcccaga gggtcaattc aatgtattgg gtttgggtca aacatcctcg
    15061 aagggtttaa agaagggtgt cgttgtaaat atcgaggcaa cggttcagtc gattcagaaa
    15121 gcattggaag aagcagagtt gatgtcggat cgccgcatca ttcaggtttt tactggtatt
    15181 gcaggtaatc atatcgtgag ctttaactca agcggtatgg ttgcaatccg ggataaggaa
    15241 gtgggagctg gagacgtaga gcgtgtttta gaaacggcta aggcgatcaa tataccgacc
    15301 gatcaacaga tccttcatat ccttattcaa gagttcatta ttgatggtca agaggatgta
    15361 cgtgagccta ttggtatgag cggtctccgt ctagaggtta aggttcatat tgtgaccggc
    15421 gcagttagtg cagcgcaaaa tattgtgaaa tgcgtaagac gttgtggctt agaagtaaat
    15481 gacctcattt tgcaaccgct tgcttctagt ttggcggtat taacagatga cgagaaagag
    15541 cttggggttg ttctgattga tattggtggt ggaactaccg atattgcaat ttatagccaa
    15601 ggatcgattc gacatactgc cgtaattcca attgcaggcg accaaattac caatgacatt
    15661 gcaatggctt tgcgtactcc aaccattgaa gcagaggatt tgaagattca tcatggggtt
    15721 gcaaagcaag agttagcaga cgctacagcc atgattgatg ttccaggagt aggcgatcgt
    15781 gagccgcgtc caatgtcaaa acaagcccta gcagccgtga ttgagccgcg cgtggaagaa
    15841 ctgttcacat tggtccgaaa tattgtgcgc gaatctggtt atgaagatat ggtctcgtcc
    15901 ggaattgtct tgacgggggg tacagccatg atgcctggaa tggtagagct agcagaacag
    15961 gtttttttga agcacgcacg catcggtagt ccagagtatc gaggacacct tcacgaggta
    16021 ttgcgtagcc cgcgctactc aaccggtttg ggtttgttaa tggaaggcca ggcgcagctg
    16081 atgcgtgggc gtcgttcgct tcaaggagga aattttcagg gggttgtatc ccgcatgaaa
    16141 gagtggttta caggtaattt ttagaggttt ttttcgtcgt cgtcaatcgg caatccatag
    16201 gggaaagctg atttatacaa ggagggtgtt atggaatttg aaatggtaga acaagaaata
    16261 gccggcaaga ccattattaa agtggttggg gtcggagggg ctgggggtaa tgctgttcag
    16321 catatgatcc gtcgaagcgt taatggagtg gacttcattt gcatgaacac cgatgctgga
    16381 gctttgcaac gctcacaagc taaggtaaat ctgcaattgg gggagactgg cttaggggcg
    16441 ggagctaagc cagaagttgg tgcggcagcg gctgaacaag cgcgtgcccg cattgccgat
    16501 gtattgcaag gggctcatat ggtctttatc acggctggga tgggcggtgg tactggaacc
    16561 ggcgctgcgc ccatcgttgc ccaagttgct aaagagatgg gtatcttgac cgttggcgtg
    16621 attagtaagc catttgactt tgaaggtgcc aagcgtttga aagtagcgga gcttggctcg
    16681 catgagcttg aaaagcatgt tgactcactc attgtggttc tcaatgaaaa gttatttgag
    16741 gtaatgggag aagatgccga atttgataaa gcgtttgctt gcgcagacga tgtattgcat
    16801 aacgcagtat caggcattgc agaaatcatt aatgagcaag gtttaatcaa cgtagacttt
    16861 gaagatgtga agactgtaat gagcgagcag ggtaaggcca tgatgggaac tgcaacagta
    16921 tcgggaatgg atcgtgctcg tttagcagcc gaggctgcag ttgcatcgcc gctattagaa
    16981 ggggtcgatc tttcgggtgc acgtggagta ttggtaaaca ttacagcaag tcgttccttg
    17041 aagttgtcag agacccgtga agtaatggct gcgattcgtg gatatgctgc agaggatgca
    17101 acagttattt ttggaaccgt ttatgacgat agcttaggtg atgcgctccg cgtcactgta
    17161 gttgcaactg gtttgaatgg ttctcaaaca atgcaaaagg atcagcctca gctcatttgg
    17221 cgtcaagcaa ccggtactaa tgatgcaagc cccactatga ccacattggg tgattttgca
    17281 ccgacaagcc cagctgctgc gatgagccac tcagtcacca gcgcgcccac gactatggtt
    17341 acaggacagc ctgtaccagt aaatccagca gttgattatg gacaatttga tattccaaaa
    17401 gttttcagaa actcgcggga tgcagtgccc cccagcttag gtgcggatag ctccccacag
    17461 gccaaagcca tgttagaaaa gggggcggat tactatgaaa tacctgcctt tttaaggaag
    17521 caagcagact aatgaattga ccatacaata gtgagttcga ggattgctaa tatagtcgcc
    17581 cctccggacg acgaatccag cgcctgtatt agcagcccaa aaccactatt gatatggaga
    17641 tcacatgatt gcagtcggac aaaaattacc caacgccacc ctcaatgagt tttttgatga
    17701 agagcgcgat ggctgttcct tgggacctaa ttcgtttgaa gttgaaaagt taacagctgg
    17761 taagaagatt gttgtatttg cgctgccagg cgcatttacg ccgacctgtt cagcaaagca
    17821 tgttcctggt tatgttgagc attttgatgc tattaaggcc aagggagtcg acgagatttg
    17881 gtgtatctct gtgaatgatc catttgtgat gggtgcttgg ggacgcgatc tgaaggttgg
    17941 caaaaaagta cgtatgtttg gtgatggtag tgcagagttc actaaaaaat taggtatgga
    18001 atttgatctg accgctcgtg gttttggtgt gcgctcacag cgttatgtaa tgatcgttga
    18061 caatggcatc gtcaagcatc ttgctgtaga gggccctggt aagtttgaag tcagtgacgc
    18121 agcgagcgcc ctaaagcatc tctaagtatt gaagagagtc aggccaatat gcttaagcaa
    18181 aaaaccttag caaaaccaat taagaccgtt ggtattggct tgcactctgg acgtaaatca
    18241 actctgacga ttaatcctgc accagtcaat tttggaattc agtttgttcg ggttgattta
    18301 cctcagacgg ttcatatccc cgctcatgct ttggctgttt gcgatacgcg acttgcttcc
    18361 gtcattcaaa aagatggggt taaggtttca acggtggaac atttattatc agcctgctct
    18421 gggcttggtc tagataattt actgattgag gtcgatgctg aggaagtgcc gatcatggat
    18481 ggaagcgccg cttcattcct gtttttgatt gagtctgctg gaattgtgga acaagatgcg
    18541 cctcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttgca catgaagtag aggcgttgcg
    18661 tgaaattggt ttagctcgcg gtggtagtct cgataatgcg attgtgcttg atgagcatcg
    18721 tattttaaat aatgaagagt tgcgctatga cgatgagttt gtgcgccaca aaatattaga
    18781 tgcaattggc gatctttatt tagcagggta tcccattgtt ggcgcctaca cggctgataa
    18841 gtcaggacat gctttgaaca acagtctctt gcgaaagctc ttagaagatg tcagtgctta
    18901 ttccattgag acctttagcg atcgtaaggc gcctgccgcc tatttacaaa ccgccgatcc
    18961 ggctatcgtc tagttttgcg accgtttagg agttgtgcga ccgcttcccg aatactggag
    19021 ttgggcgata aacgatcata gagggattgc catgcaaggg ttgcggcacc actaaagggt
    19081 ggaggagcga tcccttcctc ggtttgcggt aaggattgtc taggccgaac tttcactttg
    19141 atactcctaa ggacccacct gtcttggctg agctctttaa tcaggctggg cattatttgt
    19201 tgtaagcggg cactaatcgc tgggcttgag acaagtaaaa aaagctcctc tgggttctcc
    19261 aatttccatt ccaccatgat ttggggagcc aattgcccaa atccaagctt ttttagggca
    19321 ttttgaactt gagaagtccg gttttcatgc cttttagcct gttccattag cttttgtaag
    19381 ccccgctctt cctggaggca gtctaacaaa gggcgggcag acttaagtat gggcttattc
    19441 atcaaatggg ggctcgcggg tgataaactc agaggctaaa attttttcat attccttatg
    19501 gtacttggtc ttattaaaac actggtcggc agcagcaatg accgcctctt aaagcaatac
    19561 cgaaaggtag ttactaaggt gaattcctta gaggctagct ttcaggcctt agatgacctc
    19621 gccttgcaag ccaaaacgac ggaatttaag gaccgggttg ctgcaggaga atcacttgat
    19681 gatttagctc ctgaggcatt tgcggccgtt cgtgaagcga gtgtgcgcgt tatgaaaatg
    19741 cgtcactttg atgtccagat catgggggca ttagcactcc atcagggcaa gatagccgag
    19801 atgggaacgg gtgagggtaa aacgctaact gctaccctgg cagtgtatct aaatgcccta
    19861 tccggtaagg gcgttcatgt ggtgaccgta aacgactatt tagcagagcg cgatgcagag
    19921 tggatggcta agctttataa ctttttagga ctgagcgttg gcactaattt atcgcagatg
    19981 gatcatgagg cgaaacaaaa ggcttacgca tctgatatta cctacggcac taacaatgag
    20041 tttggctttg actacttgcg cgataacatg gtgcaggata ttgaccaacg agttcagcga
    20101 agcttgtctt acgctattgt ggatgaagtc gattccatct taattgatga ggcgcggacc
    20161 ccattaatta tctctggaca agcagaagac catactgaac tttatgtaaa gatcaatgct
    20221 ttaccgagct atcttgatcc tcaggttggg gaagagaaat cagatggctc tggggtaata
    20281 aaaccaggcg attactgggt ggatgagaag tcacaacagg tttatctaac cgagcgtggc
    20341 catgaaaaag cggagcaggt tcttactcag atgagtttgc tcaaagaggg cgattcttta
    20401 tacgctcctc aaaatattaa tttgatgcat cacctctatg ctgctttgcg tgcacattgc
    20461 ttgtatcacc gcgatcagca ttatgtcgtt cagggcggag aggtcattat cgtggatgag
    20521 ttcacgggcc gcctaatgca aggacgtcgt tggtcagatg gtttgcatca agcggtagag
    20581 gccaaagaaa aagttggcat ccagaatgaa aatcagacct tagcgacgat tacatttcag
    20641 aactacttcc gaatgtatca aaagcttgca gggatgactg gtacggcaga cacagaagcc
    20701 tatgagttta aagagatcta tggcttagag acagttgtga ttccgccaaa ccgacccagc
    20761 cagcgcctgg atcgccaaga ccaaatatac aagacctccc ccgagcgcta tggcgcagtg
    20821 attaaggaca ttaaagaatg tagtgaaaga ggtcagcctg tattggttgg caccacttcg
    20881 attgaaaact ctgaacttat ttcaaagtta ttggataaag aaaagctttc acatcaggtc
    20941 ttaaatgcta agcaacatgc tcgtgaagct gaaattattg cccaagcagg caggcccaag
    21001 atgatcacga ttgcaaccaa tatggctggt cgtggtaccg atattgtttt gggtggaaat
    21061 gtagagcgtc aatcgatctt gattgaagca gatgattctg ttgaggctgc agacaaagcc
    21121 aaacggattc agaaacttaa agatgaatgg cagggtctgc atgaaacagt tgttaatgca
    21181 ggcggtttac atattattgg tacagagcgt catgaaagtc ggcgcattga taatcagctc
    21241 cgtggacgcg ctggacgcca gggcgatccg ggctcatccc gcttctatct ttctttagat
    21301 gatccgctgc tacgcatatt tgcgggtgat cggttgcggg ctgtgatgga tcgcctcaag
    21361 atgccagagg gtgagccaat tgaggcgggc atggtaactc gttcaattga gtctgcacaa
    21421 cgcaaagtag aaggtcgtaa ctttgatatt cgtaagcagt tgttagagta tgacgacgtt
    21481 gcgaatgatc agcgcaagga gacctatcgc ct
//
LOCUS       DDPQ01000023           12525 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_8248,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000023 DDPQ01000000
VERSION     DDPQ01000023.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12525)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_8248"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1976..2021
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4250..4306
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5385..5420
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10193..10244
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12012..12082
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2854 a         3125 c         3039 g         3243 t
ORIGIN      
        1 aatgcaaatg gcaagcgcgc gggtaggagt ttgggttctt taaacgagcc atcgtagttc
       61 gggacaaaat caacggtacc ctcatcgatc tcggccaaga gcaaattagc gatcttggtc
      121 agacgtgctt cggtataacg catcgccgca gcgccatcgc catcgcgtga accaaaatta
      181 ccttgcccat caattaaggg gtaacgtaag gaaaagtctt gagccaagcg aaccagggca
      241 tcgtaagccg attgatcgcc atgcggatga aacttaccta agacatcacc cactacccgt
      301 gcactcttga ctggcttggc atccgctctc aaacccatct cgttcatcga atacaaaatg
      361 cggcgctgta caggcttttg cccatcagcc acttctggca atgctctgcc cttcactacg
      421 ctaatggcgt aatcaaggta ggcgcgttca gcgtagaccc ccaatgaaag tgcttcatct
      481 acttgagtcc gttttttagg ggggcgctca tcaaccattg cgaagaggtc tccctgatcg
      541 gaagctctct tggtcttact agttttagtg gtggtatttg gttttttgct gggcatgctt
      601 tgtcctaaat atcggcctcg acctcattac cgcgctcttc taaccaatcg cgtcgggcac
      661 ctgattcaga tttacccatc aacatatcca tcgttttaat cgtatcgctc tcgctcatct
      721 cccctaagct catgggtagg agtctgcgcg tatccggatt taaggtggtg tcccacaact
      781 gctcggcgct catctcgccc aagcccttaa agcgtgagat ctgccaggca ccatcgcgca
      841 cgccgtcttt acgtaattta tcttcaatgg cggttagctc actctcatcg agcgcataaa
      901 tcttttgtgc aggctttttg ccacgcgcgg gcgcatcaac cctaaacaag ggtggtctag
      961 cgatatacac attgccattc tcaatcagtc ttggaaagtg tttataaaag agtgtgagta
     1021 agagaacttg aatatgcgag ccatccacat ccgcatccga taggatgcag atcttgccgt
     1081 agcgcaaatt actgagattg ggttcatcgg tcggtccatg cggatcgacc ccaatcgcca
     1141 ctgcaatatc gtgaacctca ttattggcaa agagccgatc acgctcggtc tcccaggtgt
     1201 taagcacctt gccgcgtaga ggcaggatag cttgatattc tttattgcgg cccatcttag
     1261 ccgagccgcc tgctgagtcg ccctccacta ggaagagttc gttcatggca atgtcttggc
     1321 tctcacaatc ggtgagcttg ccgggtaata ctgcaacccc agaggatttt ttcttttcca
     1381 ccttttggcc tgctcgggtc cgtgcttgcg cttgcttaat cacaagttcg gccagagtgc
     1441 ggccataatc aacgtgttga ttaagccaca gctctagagc agacttcaca aagccggaga
     1501 ccaagcgcac cgcatctctt gagttaagac gttctttaat ctgtccctga aactgaggat
     1561 ccaatacttt ggccgataag atgaatgagg ctctcgcaaa cacatcctca ggcattagct
     1621 ttacaccttt gggttgtaat gcatggatct ccacaaaact cttcacagca ttaaagagtc
     1681 cttcacgcaa accactctca tgcgtgccgc ccgccggagt tggaatcaaa ttgacgtaac
     1741 tctcgcgcac tggggcacca tcttcggtcc aacaaaccac ccatgcagcg ccctcacctt
     1801 cagcaaagga ctcttcctcg ccagcgcctg aggcgtaatg ctcaccctca aatgcaggga
     1861 ttaccggggc ggtatgccca ccttgtgcca atgcttcatc cagatagcct ttgaggcctt
     1921 gggtatagag ccacgattga ctgtcaccat ttttctcttg aatgagggtg accttnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctatcaaaa tatttgggat
     2041 taggccaagc gcgcactttg gtgccatggg ctttatcccc cttggtcgca gctttggatt
     2101 tcagtttctc gatcacatca ccatcggcaa aggtgatggt tgatacctgt tgctcgcgcc
     2161 aaacacttac ctcaagacgt ttagagagtg cattggtcac cgagacccca acaccatgca
     2221 aaccgcctga gaatgcatag gcaccgccag cgcccttctc aaatttgcca ccggcatgta
     2281 attgggtaaa cacaatctcg accaccggtt ttttctcttg cggatgaatg ccaactggaa
     2341 taccccggcc atcatcttcc acgctcacgc tgccatcggt atgcagggta acgaccactt
     2401 ccttgccata gccacctaaa gcttcatccg aggcgttatc caagacctcc tgaatgatat
     2461 gtaagggatt atcggtccgg gtatacatcc caggacgctg tcgaacgggc tccaagccct
     2521 tcaagacttt gatggaggat tcgctgtatt gcgcggtttt acgagtagcc atgcgcagaa
     2581 attgtatcca taagcgagag cctggggaat aaagcgtgca aaaatggggg tatgggagcc
     2641 ttaagtcata ttcgagttct agatctaagc cgagttttag cgggtccgtg gtgcgctcag
     2701 aacttagccg atttgggggc tgaggtgatt aaagtggagc gccctaagag cggggacgac
     2761 actcgccact ggggtccccc ttttgctaaa gatccatcgg gtaacgatac aaccgagtca
     2821 gcctattaca tttccattaa tcgtaataag aaatccatca cgctcgatat cagcacaccc
     2881 gagggtcaag aaattgtgcg cggtctggtt gcacagtccg atgtggtgat cgagaactat
     2941 aaagttgggc aactcgctaa gtatggtttg gattacgaga gccttttgaa gatcaaaccc
     3001 aatttaattt attgctccat caccggattt ggtcaaaccg gtccgtatca acatcgcgca
     3061 ggatacgatt tcatattgca aggcatgggc gggttcatga gtattacagg tgaagctgat
     3121 catctacctg gcggtggtcc tcaaaaggca ggcgttgcca ttgtcgatct ctttactggc
     3181 atgtatgcca gttctgccat cctcgcagcg gtgatccatc gcgatcgcag tggtgaaggt
     3241 caatacatcg atatggctct acttgatact caagtggcca tgcttgccaa tatctccagt
     3301 aattatttat gtagcggcgt atcaccccat cgttggggaa atgcccaccc gaatgtggtg
     3361 ccctaccaaa ccttccaaac ctccgatagc tggatcattg ttgcggttgg aaatgatggt
     3421 caatttagac actttgtgca agtgggtgga cgcgagcact tagccgatga tccacgcttt
     3481 gctaataatc ccgctcgcat tgaaaaccgc gctgcgctga tccctctatt ggccgagatg
     3541 gtgaaagaaa agaccaaagc gcagtggatc tcccttctag aatcagcggg cgtgccttgc
     3601 ggccccatta ataatctcca agaggttttt gagaacgaac aggtgattgc cagagggatt
     3661 gagttacatg ttccacaccc ccgcgcgggc accatgaaac tcgtggcaag cccaatgcgt
     3721 ttatctaaaa ctccggtcga ggtacgcatg ccaccgccct tactcggtga gcataccgat
     3781 gaagtgttgc gctctgatct aaacctcacc attcaagaaa tcgcgcactt acgctccaaa
     3841 ggagtcatcg attgaacaca gcacgcaccc cttctttatc tctcgtgcag gtcttagttt
     3901 ttggtagcct gatggtgacc ctctcgatgg gcattcggca tggctttggt ttatttaatt
     3961 tacccatcac ttctgccaat ggctggggtc gcgagaccta tgccttagca attgccctgc
     4021 aaaacttagt gtggggcttt gcacaaccgg tggctggggc attggccgat cggtatggtc
     4081 catttaagat catggtgatc ggtgggctct tgtacgggct tggcttaatt gggatggcac
     4141 tcactagtga tccattcctg tttaatcttg caggcggcct atcgatcgga attggtttag
     4201 cagcaaccac ctatagcgtg gtgtacggta tcttggggcg caatgtatcn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncagt tcttaatgat
     4321 gccgctagag caagggctca ttagtagttt tggtgccaat gaagcgctga tttatttagg
     4381 cttacttgcc tcgctcatga tccccctcgc attttttctg cgcgagagta actttagtac
     4441 taatcaagga ggcaatcaaa ccatcagcga ggccctgcgt gaggcgatgg ggaatcgtaa
     4501 ttttcggttt ttgatgatgg gctattttgt atgcggtttt caggtggtct ttattacttt
     4561 gcatcttgcg ccctacctca aggatatgtc cctcaaatat ccagagatca atggtgcaac
     4621 ggttgctacc accgcactgg ctttgattgg cctatttaac gtgattggca cttactatgc
     4681 aggggtcttg ggtcaacgct ttcccaagcg ctttttacta tcgggcattt acatcacgcg
     4741 ctcagtcgcg attagcctgt ttttactgat accactgagt gcaacgagta cctacgtctt
     4801 tgcagcagta atgggtgtgc tgtggctctc taccatcccg ctcaccaatg ccatcgtcgc
     4861 ccaaattttt ggggtgaagt acctcacgat gctctctggc ctggtgttct tctcgcatca
     4921 gctcggtagt ttttgtgggg cctattttgg gggctattta tacgacctca caggctctta
     4981 ccaaatcgtt tggggcattg ctattgcctt gggggtcttt gcatgcctta ttaatctacc
     5041 cattcgcgaa gagcccttgg tgcgccctgc gatggcctaa ggcacctatt tgctttcggt
     5101 agaataagac tatgggcaag tatttattcc taagcgatac cagtgcttct tggaagaagc
     5161 gcttgggcta tctgattgca gcattggtgc ttgctttggt gttcgcgctc tactccatcc
     5221 ccgaggtggt cattggtcta gccaatcaag cttgggccat gtgtggatgg tgattctgtt
     5281 ttaacccgct gtagcacaat ggatagtgca atcgcctcct aagcgataga tccaggttcg
     5341 actcctggca gggggaccat taaagccata taaagattct attgnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn tgaagttatc cacagcgaat gtggataaat acatgaattg
     5461 ttttctaagt actgattttg tatagagtga actaggatgc acaattatta agcagtcatg
     5521 aacgtacttt tcgtgcaccc acatcactgt atttatttat agacacttcg aataaaaata
     5581 aaattgctag gtcttgcctt tttacaaaag tcgtattaag catgcgctca ttcttatcgc
     5641 ccaagttccg ataagcgctt acttggacta attgcttccg gatcaatcac gatctcgatg
     5701 attgctccct ctgggctctt cagagcagct tggattgcag gtgcgaacgc ttcggtcttc
     5761 tcgattcgat aagctggcat ggagaagctc ttggcaaagt cgataaagtt ggggttggtc
     5821 agttcggtgc ctgagacccg cgccttgtac tcccgctctt gatgcatacg gatcgtgccg
     5881 tacatgccat tgttcacaat aatcacgatc ggtttagcct gatagcgcat ggccgtagcg
     5941 agctcttggc agttcatcat aaagtcgcca tcaccgcaga atgcgacagc aacgcgtttg
     6001 ggatcaacga tcttggcagc aatcgcagca ggcagtccgt agcccatcga gccgttggcg
     6061 ggtgctaatt gggatttaaa tcccccgtag ggataaaagc gatgtaagaa ggcggcaaag
     6121 ttaccagccc cattcgtgag gattgcattg cgaggcaagt gagattgaag agcagacaca
     6181 atctcgctta attgcacggg gcctgggatg gtggtgggtt tactaaatgc tagatacgca
     6241 tcgtgtgctg cttgcattga tccgcgctca tactgtgaac ccattgtgat gctactaagt
     6301 gcctcacaaa atgcctgtgg tgatgcactg attggatagt cggcgtagta caccctacct
     6361 agttcatctg ggctgggtag cacatgaatg agtggcattt gtgtcttggg gactgtgagc
     6421 acggtgtatc cagccatggt catctcacct aagcgctcgc cgatcaccaa caaaatgtcg
     6481 gcctcttgta cacgcttgac taatgcgggg ttggctgcaa tgcctaggtc acctgcaaag
     6541 caagggtcta agttatcgag caggtcttga gaccgaaagc tcgttgccac aggtatctgc
     6601 tcacgatttg cccaggccga aagtgcatga cacgctgctt tggtccacga gccaccgcct
     6661 gcgatcacca tcggtcgttt ggctttagca aactgttgca tcgcattggc aaagagtgtt
     6721 tgatcaaggc ccgttgtaat aatctgtgcc gctggggtaa tgcgttcttg tgcgtcttta
     6781 aatagaacat cttccggaag agctaaaact acgggcccct ttcggccaga ctgcgctaga
     6841 tgaaaagcat gcgagacgaa ctcgtcaatc cgatcggtgc gatcaatact ccccacccat
     6901 ttggcgcact cggagtagac cttgcggtaa tccatctctt ggaaggcctc gcgctccacc
     6961 atatcggttc ctacctgccc aattaatacc accatcgggg tggagtcttg ataggcggta
     7021 tgaactccaa tgagtgcatt ggctgcgcct ggcccgcgcg tcaccatgac caccccaggt
     7081 ttaccggtca acttggcata ggcttctgcc atataggcag ccccaccctc ttgccggcag
     7141 gtaataaatt ttaggcggtc ttgatgatcc accaagccat ccaatagggg caagaagctc
     7201 tcaccgggca ccccaaacgc aagctctacg ccttggcgga ctaaggcatt ggctaatatc
     7261 tgaccgccat gcatcgtgtt ggagtgggtt tgactcatgg tgctctttcc ttgttttgct
     7321 aatacactat ttagatgaat agtttaacga caagtacctt ggccgccctg gaagaaatgc
     7381 tccagaacgc caaacgcaat gcaagtcaag attgtttgcc ggtgtacttt gaaaaaacat
     7441 acattggcaa tatcggtcgt ccattgattg gggcggttga agagctccta aaaaatggtt
     7501 cgtatccaca tattcagatg gacattacac gaattatttt aaagagtgca ccacccgcgc
     7561 aactcagtga tgatctgcgt gctctagcaa atgggcttca tcaggctggc ttcatccctg
     7621 cttggcgtaa tgaagagttt gcttggtatg ggcccgatgg gcatgagtac tttcgggttg
     7681 agcgggcagc ctttcgtacc tttggatttc ggagtcaagc ggcacatatc aatgggtata
     7741 cctcggcagg aacgctttgg ttaggtcgtc gcagtgagag taaacagatt gatcccggta
     7801 aattagacaa cctcagtgct ggtggcatta atgcgaatga gaccgtggtg cagtgtgcta
     7861 ttcgcgaact ctgggaagag gctggggttc ccgaagcaat tgcacagcaa attaatccta
     7921 ctgggaaatt actgattcac acccctcatc caccgcatgg tattcatcat gagtctttat
     7981 ttatgtttga tcttgagttg ccacgcaatc ttgtgccggt caatcacgat ggcgaggtaa
     8041 gtcgttttat tgaggtcgat cttgccgaag cggctgcccg tattttggct gatgagttca
     8101 ccagcgatgc tgcgctggta accgcagact ttatcttgcg gcgcaataga taatccgagc
     8161 tcgggtctag agcccttcat ccatatggtt ccatgttcag gatcgggaat tcatggttaa
     8221 atactcctaa ggatgaaaca ggggtgcccc cacatggttg tggtgctgag aaagaccctt
     8281 taacccgatc cagataatgc tggcgtgggg agttccatca gaccgacacc cggtttcgtc
     8341 cactctatat caatgcattt tgcgaggaga tggacatgag tgtgagcacc gatacaaaag
     8401 atcaaaaaag aagtaagcct gaaatcccca gcttaaagag tttggagcga gattttgggc
     8461 agaagtttgc ctaccctgct tccactaaaa cttatttaga aggctcgcgt cccgatatca
     8521 aagcaccgat tcggatgatt gagcaattgc ccaccaaaac aggtgagacc gaaacgtcta
     8581 acccacctat cccggtgtat gacacctcag gcccatacag cgatccagat atcgttatta
     8641 atttagagaa gggtcttcca aaattacgta gcggttggat tggagagcgc aaggataccg
     8701 aacaacttgc tggcccatct tcagagtatg gtgttgcgcg tgctcacgat gcagccacct
     8761 cgcatttgcg ttttgcccat atcgccgctc cacgagttgc taaatcaggt gcgaatgtta
     8821 gtcagatgca ctatgcccgc aaaggcatca tcacccctga gatggaatac gttgccctac
     8881 gcgagtcctt aggtcttgag aagctccgtc aagatcctcg ctatacccaa atcctcaagc
     8941 agcatcctgg taaatccttt ggtgccaata ttccggatgt gattactcca gagtttgtgc
     9001 gctccgagat tgcagcgggt cgcgccatta tccctgccaa catcaatcac cctgagttag
     9061 agccaatgat tattggtcgt aacttccgtg tgaagatcaa tggcaattta ggcaactccg
     9121 cagtgacctc atcaatcaat gaagaggtcg agaaaatggt ctgggcgatt cgttggggtg
     9181 ccgacaccat catggatctt tccaccggta agcatattca tgagacacgt gagtggatta
     9241 ttcgtaactc tcctgtacca attggtacgg ttcctattta tcaagctctc gataagaccg
     9301 gtggcattgc cgaagacctc acctgggaaa tgttccgcga tacgctcgtt gaacaagctg
     9361 aacagggtgt tgattacttc accatccatg caggcgtctt actccgttac gtaccactga
     9421 ccgctgatcg cattaccggc atcgtctcac gcggtggctc gatcatggcg aaatggtgtc
     9481 ttgcccacca taaggagaac ttcctttaca cccgctttga tgagatctgc gagatcatga
     9541 aggcgtacga cgtctccttt agcttaggtg atggtctacg cccgggttgt attgccgact
     9601 ccaatgatgc tgcgcagttt ggtgaactcc ataccttagg tgagctcact gccaaagcct
     9661 gggagcacga tgtgcaagtg atgattgaag gccctggtca cgttccaatg cagcgcattg
     9721 aagagaacat gaccgaagag ctcaagcatt gcttagaggc acctttttat acccttggac
     9781 ccttgatcac agatattgct cccggttacg atcacatcac cagtggtgtg ggagccgccc
     9841 aaattggttg gtatggcacc gcaatgcttt gctatgtcac accgaaagag catttaggtt
     9901 tgcctgataa ggaagatgtg cgcgagggca tcattactta taagattgct gcccacggtg
     9961 ctgatttagc caaaggcttt ccaggcactc agttacgcga taacgcactc tctaaagcgc
    10021 gctttgagtt ccgctgggaa gatcagttca atcttggctt agacccagag cgtgctcgcg
    10081 agtatcacga cgctaccctg cccgctgaag gtgcgaagat cgcccacttc tgctcgatgt
    10141 gtggtcccaa gttctgctcg atgaagatca cccaagatgt ccgtgattac gcnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcaaa cgcggtagcg
    10261 agatctatca gtaaggatct ttgcatgtct cggtcctacg ccatcctcgg tgccggcctg
    10321 atgggtcgga tgatggcgta tgcccttgcg caaggtggag cacgcgttga gatgttcgag
    10381 cgcagtggcc ctgatgcaga gcaatcggct gcacgggttg ctgcctcgat gcttgctcct
    10441 ctagcagagt ctgcaattac cgaagcatcg gtggtgcgca tgggtctgta tggtcttgat
    10501 cgctggaaga gcttgattga gcaactcaat aacaaggttg aacaacagac cttttttcag
    10561 caagacggca ccctagtctt gtggcatcgt cttgatgctg ccgaggcgca acgctttgct
    10621 gagcatctnn agtgtggcgg agcgcttttc acaaggtttg ttcttgcccc gtgaagggca
    10681 gttggataat cgccagttat taacggccct cttagaagca ctccagcagt tagatgtgaa
    10741 gttgcattgg cataccgctt gcgaacccga tgatcttgca gatcacggtt ttgattgggt
    10801 gattgattgt cgtggcttgg gtgcaaaatc gtcttggtct aatacacata atccattacg
    10861 cggtgtgcgg ggtgaggtca ttcgtgtgca tgcgcctgaa gtgaaattgc aacgccctac
    10921 tcgcttaatt catccgcgct atccgattta tattgccccc aaagaaaatg atctctatgt
    10981 tattggtgct actgagattg agtctgagga tctctcaccg gtcagcgtac gctcttcaat
    11041 ggaactctta tcagctgcgt attcggttca ctctggcttt gcagaagccc gtattttgga
    11101 atctgcaacg caatgtcgcc ctacgctcaa gaacaatttg cccgagatct gtgtaccacg
    11161 tgccggcata atgcaaatca acggcttgta tcgccatggt tatttaattg ctcctgcggt
    11221 gatggacgcc gcacaagaac ttcttcacga aaccggtagt gccttagcga agcgctttga
    11281 gattgccatc cagcatcatg atctaaccag ttcttttgca tgatgcgcgt actcgttaac
    11341 caaaatcccc atgagcttgc gcctgcttcg aaggtgagtg atctcttggc tctcatcgat
    11401 gccaaaccgc cgtatgcggt tgccgtcaac ttgcagttct tgccaaagtc gcaacatgcg
    11461 gactatgtac tgcaagagaa cgatgcggtt gagatcatct ccccagtaac cggtggttaa
    11521 gctttttatt ctgattattc ttttatgacc gctcctcttc cttcccatct caacgatcca
    11581 ttaatactgt atggtgagtc ttttgcgagt cgcttattac taggtacttc tcgctatcca
    11641 tcgcctcagg ttctcgagac cgcggtgcaa cgcgcgcgcc ctgccatgat tacagcgagt
    11701 ctgcgccgtc agggctctgc tgcaacagaa gcgcacagcg gcttttggga actcctcaag
    11761 aaaatggcag tcccggtttt gcccaatact gctggctgtc atagtccgca agaggtcatt
    11821 accaccgccc aaatggcgcg tgaggttttt gagacccact ggatcaagct cgagttaatt
    11881 ggtgatgact acactttgca acccgatacc ttgcgccttg ttcaaaccgc tgagcatctc
    11941 attaaagatg gctttaaggt tttgccctat tgcaccgagg atctcatttt gtgtcagcgt
    12001 ttggtcgatg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnctcaatcc ctatgctctc aaattattac gcgagcgcct
    12121 atcggtcccc ctgcttgtgg atgcaggtct tggccttccc tcgcatgcgt gcacggtaat
    12181 ggaatggggc tttgatgggg tgttactcaa tactgctgtt gctctagccc acgacccagt
    12241 atcaatggct aatgcctttg cacaagcagt tgatgcagga cgtcaagcct acttatctgg
    12301 tgcgatgcaa gcgcaagact ctgcgcaagc cagcactcca ttagtcggga ctccgttctg
    12361 gcatcaagat caaggaacta actcgtgagc catatccgcg agttagcaaa acacattgtg
    12421 gcggcgcatc gcaatgatga tctttgtttg ccattgcccg cttttagtcc cagtaatcct
    12481 ccgccggtga taaagaccga gggcggtcaa gatcaatatg aatta
//
LOCUS       DDPQ01000024            2611 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_17402,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000024 DDPQ01000000
VERSION     DDPQ01000024.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_17402"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    596..690
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1614..1663
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          717 a          579 c          525 g          645 t
ORIGIN      
        1 aagcttattc gtcctggtaa agatcaaagt ttttctaatc catcctctca agttaattaa
       61 ggagtttttg catgatgaat cgtgacgttg taacgcaaaa aattattgaa gccaaagtaa
      121 agaaggggct taaatgggct gatattgcca aatcgattgg cgagtcaaaa gagtgggtaa
      181 ccgctggttg tttgggtcag atgaccttta ccaaggctca agctgaagcg gcaggtaagt
      241 tatttaatct caccgacgag gaaatggctt ggctccaaat agttccatac aagggctcac
      301 taccaaccgc agtccccaca gatcctctta tttaccgctg gtacgaaatt gttagtgttt
      361 atggcactac gatcaaagaa ttaatccatg aggagtttgg ggatggcatt atgagtgcga
      421 ttgatttctc gatggatatt cagcgtgagc cagatcctaa aggagatcgc gttcaagttg
      481 ttctatccgg aaaatattta tcctataaaa catattaatg cggttcttac aatagcggtg
      541 aggctaggtg tgccgtattc tctaagagct tacgccacct tggtctgcgc tcccannnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttatcaatca acaagctctt ggtatgcaat
      721 agtccacctt taaattcggc aatctctacc cctgcattca gtaatatgct gaagaaactt
      781 cgactactcc aggccactaa ggtggaatca tttagcttcg gaacaatcag cttgacctta
      841 actccccggt aagatgcggc cattaatgct tggaccaagc cctcgtcggg tacaaaatag
      901 ggggtcgtaa taatcaactc ttcgcgagca tctaatactg cggaaagtaa aacctgatac
      961 aaaatatcat cacggttaac aggaccagag gagaactcct gtgccaaagc acttcccact
     1021 actttcttat tgagactctc gcgatcatta aagtgcacaa cccttggatt atcaacactc
     1081 caatcatata aaaaggtaag ctcaaactga gatacaaccg ggccctggat tctgaccatc
     1141 gcatcgaccc actctcctac tcctgagtct tgcttgaatg ttctcgggtc gaccaagttc
     1201 atactccctg tccatgcagt ttccccgtca atgacaaaga ttttgcgatg caagcgcaga
     1261 tcagcacgcc tgaactgaaa acgaccaaac tgaatgggca gggcctcggt gacttcaata
     1321 ttggcatcct gaaaacgtct cggccatgca gacttaaacc atttggcact acctaaggag
     1381 tcgagcagaa ctctgcattt aatacctcgc tttgatgctg caatcaatgc ttcagccact
     1441 cgatctgcat cgccaccaag ggcccaaata taaaactcca gatgcaatga tgttttggca
     1501 ctatttattt cgtttatgaa atgctgcaat atctctaatg aattagtaaa gagctctatc
     1561 tgatttcctg cagcaaccgg tgctccattt tttgcttgag ctagtaggct aatnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctacca acaggtcgct
     1681 ctccgatggt gagataaaga atcaggccaa tcgctgggaa ggtcacaata atgagtaacc
     1741 aagagattgc aacactcact ggccgtcgaa tccaaataat ccgaagaaag aaaaggatgg
     1801 tgacaatcag gtgcaggatc aaaaaccatg ggattgaata ccccattaag cctcctgaga
     1861 ataaatgcag catcttcgtg tcctatccta atgacctaat tcagcataaa tagcgagatg
     1921 atctgatagc ttagaccatt cggtgaggat ttctgctttt tgaatcgata agcccctgac
     1981 ataaatacga tccatcggta atacgggtcg aacactcgga aaggtccttg caggggcgcc
     2041 atacagactc tcaaagacat ccacaaaccc agcctgcccc atcggcttgc taatcgtatt
     2101 acgccaatca ttgaaatcgc cagcaatgat cgtgggatga ttgcccgata gatgttcaat
     2161 atgatgcaag atggtcgtaa gttggcgctc ccgacctcgc tgcaaaagag ccaggtgaac
     2221 acaaaaacaa tgaatagtct tctgtccctc tagctcaatc gtactgtgta gtaagccacg
     2281 tctctcaaac cgatactccg aaatatcgta attgatacct ttatgcaaag gaaactttga
     2341 caagatcgca ttaccgtgat gcccatgtcg atattcggca ttctttccat agtgccagtc
     2401 tggatagatg ccatcagcca gaaaatgggt gatctcatgc tttggccaac tggcaaagcg
     2461 acgctgacgt cgcacgttct cttgctgtag ttcctgaatg aagattagat ctggatgatg
     2521 cgatcgtaat ttttgacgca aacgataaac cgtagagtct acctttagtg gcgaaagccc
     2581 tttatgcata ttgagcgtga tcacgcgtag t
//
LOCUS       DDPQ01000025            3228 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_15854,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000025 DDPQ01000000
VERSION     DDPQ01000025.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_15854"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1387..1474
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2404..2438
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2785..2806
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          794 a          706 c          662 g          921 t
ORIGIN      
        1 cccgatcaaa cacagcgact tcattttcgt gggctagccc ctgccgggcg tcttgatatt
       61 gattcatctg gattgctggt actaactcag gatggtcgca ttgctaaatt attaattggg
      121 gaagatagtc cgattgagaa agaatattta gtgcgggttt ctggtcaact ctctgatgca
      181 ggcctgcgct cattacaaca cggtctatca cttgatggca aagcgcttaa gcctgcgaaa
      241 gtaagttggc aaaacgagga tcaattgcgc tttgtattgc gcgaagggcg aaaacggcaa
      301 atccggagga tgtgtgaatt agttaatctt gaggtcattg gactaaagag aatccgcatt
      361 ggtcagcttg cgcttggttc gctccctgta gggcagtggc gtgtgcttgg taaaaatgaa
      421 cgcttttagg tcttcactgc aaataaagta agctccatag cgcgctctcg aactggcagc
      481 aatgctgagt attctggact ttctgcccaa cgtttcgcat cgtccatact cggaaatgag
      541 agctcgacaa aggtatcaaa agaatttgta tttaattgat tccaaaatgg ttctgcgcac
      601 tcgcctcggc ggactaccga tccaccatat agtgcaatgc ttaagccaac ctggcttcga
      661 taaagttcga agtcctctaa agaaagtgtt ttaaagattc cgacgactgt aatactcata
      721 tagccctact tgtttgttgc ttacttcgat aataaccgca gatagcgctg atttagctca
      781 ttggaatcgg tgacggttgt gtctagatcc atcaggaccc catcacacaa gctataaata
      841 catccatgga ttgagatccc ctgtcccttt gaccaagcat cttgcacgat attggtttgg
      901 ccaatgttaa ctacttgctc aagcacattg agttcgcata atcgatctag tcgtttatgt
      961 tgatcatgaa tattactaag ctcacgatta tgtttgtcat gaacatcttt aatgtggcgt
     1021 atccaattgt cagcaatccc aatccgaaca ttattcattg cagcctcaat gccggaacag
     1081 ccataatgcc caaccacaat aatatgtttt acttttaatc gatcgatcgc aaactgaatc
     1141 actgataagg cattgaagtc ggtatgcacc accatattgg caatatttcg atggacaaat
     1201 acttcaccag gctttaatcc aacgatttgg tttgctggca cgcgggagtc tgagcaaccg
     1261 atccataggt actctgggtt ttgttgatcc actaagcgtg aaaagtagtc aggatcatcg
     1321 tcggtgacct tcttaatcca tgaacgattt tgttcaaata gccgctcaag atctagtttt
     1381 ttattannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttatt gatctggtct cttgttgcga
     1501 attggcttaa aaataaaaaa ccacccgaag gtggttttaa gtgggctaag caatcagact
     1561 aagcagcagc tttgcttcct gatcccaaga actctacaat ctttttagcc gtctccatat
     1621 accgtgcatt cattttgcga tattgattta gctcggtcat tattggattt tctgccgact
     1681 caaaggatgc gtcttcatat ccagggtggt aaggtgcctc ttgaactaat ttgctattca
     1741 tgaatcctcc aaaggttaaa taatttgata ggttcaatgt agcatagtga tttgaaaatt
     1801 aaatctttat aaatcaatta gttatataaa taatgtagcc catctgcttg ttttcattgc
     1861 atgtgatttc atttttcagc aagttgtttc atattacgaa attactcact ttcttctcct
     1921 tcgccgcttc gctttagtct tggccctcct gagagttggc aatggccgcc ataccctctt
     1981 cgatcgaata gggtgctcga tacgaaagaa tgcgttttgc cttctctatg gagagtgtca
     2041 ggggatagcc cgtcgctcga actgcctcgc gaactaaggg cggatcaccc ttcagtggta
     2101 aataattcca accattctcg gtaaatcgtg caaatggcca ggccaatgct ctgggaattg
     2161 agaatgttgg cacgcggtaa tgaccaacgc gcaaacgctg agtcatccag tcacgaaagc
     2221 gcagggtctc gttgtcagct ataaaaaatg cttctgccct caccgagctt tgcacggcca
     2281 aagaaattgc ttcgcataaa ttaccgatat aacagcttga atacaaatac tctccttgat
     2341 taaaccaacc gaactttcca tgattagcag caggcccaat ctgtcgatcg actaggtctc
     2401 cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc atagggcatc tcattgcgat
     2461 tacacaatgg cagactctct tctgcatcgt atagtgggcg tggttcattt agaactacac
     2521 tcgccgtact catatgaata aaacgtttta ttcctgccac ttgtgatgct gcaattaatt
     2581 tcttggtgag ttcgacgttc actccttgaa atgttgctcg tggaccccac aagcgcacat
     2641 acgccgcaca atgaatcacc gcatcatggc cctttagtgc tgcatgtaga tcgatttcat
     2701 cgcataaatt acctctgact gtctcaatcc ccagcgcctg caatcgtttc tcttcttgaa
     2761 ctgaacgaac ggcggcgcta agccnnnnnn nnnnnnnnnn nnnnnntcat ctttgctatg
     2821 ctagcctact taagcatttt tatattctga ttgagttctt aatgagcatt tactggattg
     2881 cactcttgct agctggactt tgtgagattg gctggccact gggcctcaaa ctatccgatc
     2941 tgcctaatat gaaagcctgg gggcttctat tggccattgt tagcatggcc ctcagcggat
     3001 tattgctgtg gtactcgctc aaagagatac caattggtac ggcttatgca gtatggactt
     3061 cgattggcgc agttggcacc tttaccattg gcgttctgat ttttggagac cccaatattg
     3121 caatacgctg ggttggcgta gccctaatcg tgctgggcgt cgccttgcta aagcttggtt
     3181 agagacgcta ctatgtagtt cccagcaaat taagacatgc tagccgtt
//
LOCUS       DDPQ01000026            4006 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_22871,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000026 DDPQ01000000
VERSION     DDPQ01000026.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_22871"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    122..209
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1078 a          963 c          850 g         1018 t
ORIGIN      
        1 catcttttca tgaaacctta gatataccca gattatcttc gtgggcagtg caatttatgt
       61 tgccgtggcg tatgccaagg gtgaaataaa ttaacttgtt tgactaatct ctaaaagcca
      121 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ctacatttag ctgataacca ttcattctta
      241 aaaatacgtc cattgcggca aaagcgatcc tcttatttcc atcaataaat ggatgattaa
      301 taatcaaact ctcaaataat gccgctcctt gcaaaagcgt atctgcataa tagccagact
      361 gtggacgaaa aacggctgcc tccaaaagat tagcgtcacg aaggcctagc gcgccgccat
      421 aacgctcaat caggacgtga tgcatcactt cgacatcgtg tgtcgttaaa taactagaca
      481 ttgactgaaa aaattacttt gctaactttt gataaagcgc atcaaattga tttaagctct
      541 gggcaaaatg attcatggca ttgcctttgg actttagaac gcccttctta acgagataat
      601 cgctgagagc ctcatccaaa atagaatgca tttttctacc ctcacgctgt gcaagctcct
      661 tcatggcatt gaggatttca gggctggcct gagacgagaa tttactaagt tgctgattgg
      721 gcattatcta cactttcaag aattatcttg attattcaag aaatttcatg aaatgtcaag
      781 cccttttcag ccaaggcctc aagcccgaaa ccggtcctca acactgagtt ccgcttcgcg
      841 ctttcgctcc tcaacacgct ccagcagatc gactacccac gtaagtcgct cataaatagt
      901 tccaccgtag tgacgaatgt gcggtgtttt gctgactgca tcaatcaaaa actgaatcga
      961 gcgatgggcc tcttttgcca agcggcgatt cgagaactta gcaatgatcg ccgaggctgc
     1021 gtgtgtcagc agaactaatt tacgctgctt cgagaccacg gcgcgtaccg atacagaatc
     1081 aagatttaag cgttctagct cacgaccgat ctcggcgtaa atcaaacggg ctgcctgtat
     1141 agcgggtcga cagtcccacg gtaactcact aatgccaatc tcagcctgtt tataaagtag
     1201 atctgcttct tttaataatc gcgccactac tctggaaagg gcttcattaa attgagggtt
     1261 ttgtaaccac tcttctttgt taataccttc ttctcttagc cattgcaagg gcagataaac
     1321 acggccattt aaagcatctt cacccacgtc acgcgcaata ttcgttaatt gcattgctaa
     1381 accaagatcg cacgctcgcg ctaaggtctc tggctcgcgt ttacccataa tcaaacacat
     1441 catggcacca acagtacccg caacgcgtgc gcagtaatct aatagatcat caaaggtctc
     1501 ataagtccgg tattgcgtat cccactcata accttcaatt aaggcaagag gaagctcttt
     1561 cggaatatca aatgctctaa ccacttcgga gagtgctcga tctgcaggaa catcaccggg
     1621 gtctcctgca taaatcgcat caagccgctc tcgcagggta tggatagatt ccgcagtggc
     1681 atcggccgca tcggcaatgt catcggtaat tcggcaaaag gcatataaag cagtagcagg
     1741 atcgcgcacg cgacttggta ggacacgcga ggcagcaaag aacgattttg agccatcgcg
     1801 catggtctcc acgcactgat tcaaatcatc atcaaaacgg ttagagctac gattcatttg
     1861 gcatatcctg tcgcaacgtt gattgggtgt ggcacgacag acatcaaagc cttagcagac
     1921 gacaacacgc ctggtatccc tgccccagga tgtgtgcccg caccgaccat gtataaaccc
     1981 tttacatcct cactgcggtt atgaggccta aaccaagcac tttggagaag caatggctca
     2041 aaaccgaagg ctgcgccttt tacagataac agtcgatcct gaaaatcttg cggggtcatt
     2101 aagaaagagg tggtcacatg atcttgaaag cctggaagaa cggattgatc caaatactgt
     2161 gcgattgctt gtcgataagg ctcggcctgt tcttgccagt ttgtaccact atctaaatta
     2221 ggaacaggtg agagcacata gaaagtgtca tgacctgctg gagccaaact cggatccgtt
     2281 gccgtgggac gatgtaaata tagactaaag tcttcggcca aaaccttacg tttaaagatg
     2341 tcggttaata actcccgata acgcttaccc aacaaaatca tatgatgagg tacatcctga
     2401 tattgacgat tagtaccaaa gtaccaaaca aataagctca tcgagtattt actacgttcc
     2461 acttttttat cggtccaaac tcgacggtgt tgtggctcaa ttagtttctg gtaggtccat
     2521 gcagcatcgg cattcgaaac tactatatcg gcaggtacaa aatcgccgtc tgctaagcta
     2581 accccaactg ctttaccatc acggacctca attcttttta cttcnnnnnn nnngcaccgg
     2641 ttcctcccat ggcagagaag actccatgtt tgcgttctaa agaattaatc agtgaataca
     2701 cggctgtgac cgagaatgga ttgccgccaa ttaatagagg atgaaagctc atcacttgac
     2761 gtaatttgtc attctttaga tgacgcgcta ctaaggtgta aatactttcc cacgctttca
     2821 tcttaaccat ggaaggaagt gcttccgcca cgtcgctcaa ggaatcaaac gcaacatgac
     2881 tgagctcttc aaagcccagc ttatagcact tctcagcctc tgccaaaaag cgatcgtaac
     2941 ctggtaaatc ggaagggcta aacttggcaa cctcttcgcg catgcgctgt aaatccccgg
     3001 tgtaatcaaa gtgagtgccg tcgtcaaaac gaatacggta aaagggatcc atcaagcgca
     3061 aatcaacatc ggtctctagt ttttcaccgc acagctccca cagctctttt agaagaaatg
     3121 gtgctgtaat aatggttggg ccagcatcaa aggtatagcc atcgcgttga tgtacataag
     3181 cgcgcccgcc aggcttatcc agcttctcta aaatctttac gcggtaacct ttatgggata
     3241 gtcggatggc tgctgctaaa ccaccaaagc cactgccaat cactaccgca ataggccgat
     3301 cgtcgctagg aaagtatgta cgtttgagca tgtatctgtg ttggagaaaa aaatggcgcc
     3361 acgaggacgc catcaattac cggttacatc atcttcactt catcgctact gtttttttct
     3421 catctgactt tggtgccgcg cgcggtgccg gtgtgccagt taagaaagga tagtctttga
     3481 gcatcgatac gccatacagt ggtttatatg ccccttggtg acaagtcgcg caattggcct
     3541 ttgccacatc tccttccggt cccaggcgat gcgcagggaa tacgttggtg attggaacca
     3601 tatagttgtt gttaatatca cgtgccatcc ggataccgta ccaagcagtc atccgttgcg
     3661 gtgggctttg cgaccactct ggcatcgaac gggtgttatg gcaatacgcg cagttcacgc
     3721 ctaaggactt cgacatatgc accatcaaac caaagacctt ctcggtatcc tgaatgctgt
     3781 tcttattacc ggttggtaaa gcagtgggtc cattcatgcg aatgttttct gcttttaaca
     3841 aatagggtgt aaagggatcg taaggaagat cgcttaaccc aaccgatggg gatggcgtat
     3901 tttgaccatt cttaccactt aagaacccac taccctgagg tttagcaggg tcagtaaacc
     3961 agacgttttg tggaatattg ttaccacgat ggcaggtata gcaggt
//
LOCUS       DDPQ01000027            4947 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_15242,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000027 DDPQ01000000
VERSION     DDPQ01000027.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_15242"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3865..3966
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1327 a         1013 c         1242 g         1263 t
ORIGIN      
        1 gcaagttact tccatgcgaa tcgttgggac tatgtctttg agttcaagcg tgagggcgag
       61 actgttggta aggagcgacg tgtcacatta atttttgagg gagatcaact cgctaagttt
      121 ggaggggatg ccctaccaac cgaagttgaa ttggtggcag aaattgatac ctacgcacgg
      181 gagagacgaa gtttttggga tgtgatgact ggcaaaaata agccccccaa aagagaaaaa
      241 aaggtggccc ctgaagtgat ggtaattagt cagccaaata atttaccctt gccatcccca
      301 cagccaaata cagtaattac gcctgttaaa aactagtgga tagaaggtaa atcatgccaa
      361 tgaaatttgc ggttgccggc gctaccggaa agatgggcaa gatgctcatc gagaccattc
      421 tgaatgatcc aaatgcggag ctaactggcg cactcgagca tagcgcatgc ccacaattag
      481 ggaacgacgc aggggcattc ttggggaagg caacaaaggt ccaaataact tctgatctag
      541 ccaaaggttt gacaggggct gagtatttga ttgacttcac aagacccgaa ggtactttat
      601 tgcatgcggt tgcggcacaa aaggcggggg tcaaaatgat tattggaacc accggtttca
      661 cccctgagca attaaagcag ttacaggcca cctcagaaac cattgcaatg gtctttgcgc
      721 cgaatatgag tgtgggtgtg aacgctacct tcaagatttt gcaggtcgct gcgcagttgc
      781 ttaatcaagg ctatgacatt gagatcgtgg aagcccatca tcggcataaa gtggatgcac
      841 cctctggcac tgctctaaag atgggtgagg taattgctaa tgcacaaggt aaaacattaa
      901 gtgatgtagc agtgtatgca cgtgaaggcc atactggtcc aagagtgcca gggagcattg
      961 gctttgccac catccgtggc ggcgatattg tgggtgatca tacggtgatt tttgcgggtg
     1021 agggtgagcg gattgaaatt agtcacaaat caagtagtcg tcagtcttac gctcaaggtg
     1081 ctttgcgggc agctgccttt ttacagaatc aagcgaaggg ttggtttgat atgcaggatg
     1141 ttcttggcct caaaaaataa ccacatcatt taaaaacaaa taaaaattta gagtaagacg
     1201 gcgatggcaa aggattatga ttttcgtagt attgaggcaa atgcacatag cgattggatc
     1261 aaaaatgagg cttatcgcgt aatcgagaat gcaaagagtg cagacggtct ggtaaagcct
     1321 aagttttatg cttgctcgat gttgccttac ccatcgggta aattacatat gggccatgtg
     1381 cgcaactaca ccatcaatga tgtaatggct cgtcagttac gcatgcaagg ttataacgtt
     1441 ctaatgccca tgggctggga tgcctttggc atgccagcag agaatgcagc aattcagaat
     1501 aaagttccac ctgcgcaatg gacctatcaa aatattgatt acatgcgtaa gcaaatggct
     1561 gcgatgggat tagcgattga ctggtcacgc gagatcgcta cctgtcagcc ggattactac
     1621 cgctggaacc aatggttatt tttgaagatg ctagaaaaag ggattgcgta tcgtaagacc
     1681 caaacagtga actgggatcc cattgatcaa accgttctgg ctaatgagca ggtaattgat
     1741 ggacgtggtt ggcgttctgg cgccccagta gaaaagcgtg agatccccgg ctattacctc
     1801 aacatcaccg cttatgcaga agaactgctt acgggcttag atcatttaga ttggcctgaa
     1861 cgcgtcaaga tcatgcagca aaactggatt ggcaaaagtc agggggttcg ttttgcattt
     1921 gctcatcaaa ttaaagatga tgctggtcac cttattggtc aaggcaaaat gtatgtcttc
     1981 accacgcgtg ccgataccat catgggcgtt acattttgcg cagtggcggc agagcatccc
     2041 ttggctagtc aagcagctaa atcaaatcca gccttggctg gttttattga gaagtgtaag
     2101 accgggagcg tgattgaagc agatcttgct acgcaagaca aagagggcat gccaactggc
     2161 ttatatgtaa accatcctgt aacgcaagag ccaattcctc tatgggtggg caattatgtt
     2221 ttgatgtctt atggcgacgg tgcagtgatg ggcgtgccgg cacacgacga acgcgacttt
     2281 gcatttgcaa ataaatacca gctcccaatc aagcaagtca ttgcagtaaa gagcaaaact
     2341 tctttattta acgcaagcca ttggcaagac tggtatgccc aaaaagataa tgttgagtgt
     2401 atagatagcg atcgctacga tggcttatct catgaagagg cggtccaagc tgttgcgcaa
     2461 gatctcaagg caatgggatt gggcgaactc aaaaccacct accgcttgcg ggactggggg
     2521 atctcccgcc aacgctattg gggaacgcca atcccgatta ttcattgtga gagctgtggt
     2581 gaggtcccag ttcccgaagc cgatttgcca gtaatacttc cagaggactg tgtacccgat
     2641 ggttctggca accccctaaa taaacgagat gattttctaa aagtggattg ccctaaatgc
     2701 ggcaaatcgg ctaggcgcga gaccgatacg atggatacct ttgtcgattc gtcatggtat
     2761 ttcatgcgct atacagggcc tgatgcaaag acgatggtcg atgcacgtaa tgagtattgg
     2821 atgccaatgg atcaatacat tggcggtatt gagcatgcga ttttgcattt gctgtatgcg
     2881 cgcttttgga ccaaggtcat gcgcgatatg aagctcatcc actttaacga gccttttcag
     2941 aatctgctaa cccaaggtat ggtcttaaac gaaacctttt ataaagaaga tgccggtaag
     3001 aagctctgga ttaatccctc ggaggttgag cttgatttgg atgaaaaagg ccgacctatt
     3061 gccgctaaat taatcaagga cggctcgcct gtagagattg gtggcgttga gaaaatggcc
     3121 aagagcaaga acaatggggt tgatccacag gctctcattg atcaatatgg tgcagataca
     3181 gcgcgtttat ttacgatgtt tgccgctccc cctgagcaac agctcgagtg gtctggggca
     3241 ggggttgagg gcgcttcacg ttttctgaga cgactctgga tttatgcgag ctctcaggcg
     3301 caggtacttc acaatgtcca aacagtaatt actggtgaac taaatacagc tgagaaggat
     3361 ctgcgctttg aagtgcatac cattctcaag caggctaatt ttgactatca acgtcgtcaa
     3421 tacaatacag tggtctctgc agcgatgaag atgttaaatg ttttagagcc cattaagtta
     3481 gatggcgcat cacaacaaat tagtccagct gttctatctg agtctctcag tattttgttg
     3541 cgtattttat atcccgtagt tccccattgc acttatgtac tttggcagga actaggcttt
     3601 acaaaacagt ttggatccct attagatgct ccatggccca cggtggatga atctgctctg
     3661 gtgcaaagtg agatagagtt ggtattgcaa gtcaacggca agcatcgagg gcagatcaag
     3721 gtacctgcag atgccaccaa agagataatt gagtctattg cccttcgtgc cgagtcggtt
     3781 cttaggatat tgaatggcgc taatcccaag aaggtcattg tggtaccggg tagactcgtg
     3841 aatgtcgtag catagcaata tgagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnngagg atcggtcacc atcccctata aagcgatttt gatttctggc cgtccttcgc
     4021 cccagttacg ttatgacctg gagcgcatca ttgtcactgg ctcaaatgca aaagtagtaa
     4081 gcaatggcaa agatgccgat ctaattttgg agattgtgag cgaggagagt acaaggcaaa
     4141 ttttgaccta cacttccgct ggtcagatct ccgcttatcg tcttaataat cgtgtgattt
     4201 ttagagcctt tgataatgtt ggtgctgagg ttattccaga gtctgatatc tatgtgaccc
     4261 gcgatttaga tttcacgacc gcaaccgttt tggcatctga tcagcaacaa cagcagttct
     4321 tagaaagtat gcgcgcagat cttgctttgc aaatattgcg gcgagtcgca acgcttggtc
     4381 gggtagcaag atagctcact atttacccta ggaaagtggc atgatcaaaa gcgacgcatt
     4441 tcatatgcat ttgactgccc ttgcaaaaga gcagcatagc tttaagcccc tttatatttt
     4501 tagcggtgat gagcccttat tgatgatgga ggcaatcgat gccttgcgag ctcttgctag
     4561 gactggtggc tttactgaac gtgaggtgtt ggtgcaggat cgctattttg actgggccgc
     4621 tctaatcaat gccgggcaaa ccatgtcttt atttggggat aagcgttttg tagagctgcg
     4681 tatacctact ggtaagcctg gtcgagatgg cgctgaatct ttaaaacact ttgctgagca
     4741 aattaacaat acaggcaatg gtgtggacac gatcatttgt attattttgc cgcgcttaga
     4801 ttcgaagacc aaaagttcag catggttctc cgctttagat gaagctggta tggccataca
     4861 aattgactca attgatcgcc ttgcccttcc acattggatt ggacaacgcc ttaaaaaaca
     4921 atctcaggaa gtggaggcag gtgaaag
//
LOCUS       DDPQ01000028           25657 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_4947,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000028 DDPQ01000000
VERSION     DDPQ01000028.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 25657)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25657)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25657
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_4947"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1325..1344
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6050..6149
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7020..7106
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8474..8587
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13328..13367
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17879..17940
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18481..18499
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7158 a         5850 c         5484 g         6710 t
ORIGIN      
        1 tttatcttac tggatgagcc atttgccggc gttgacccaa ttgcagttgg tgaaattcaa
       61 cgtattgtac gtttcttacg ggaccgccaa atcggggtcc tgattaccga tcacaacgtt
      121 cgtgagacgc tcggcatttg tgaccacgcc tacatcatta gcgaaggtag cgtgctagca
      181 gaaggtaagg cggatgaaat tatccaaaat gatgcggtaa gaagagttta tttaggtgaa
      241 aacttccgca tgtaatcgtt ttggcattga atcaaccagc cgaaaagact aaacctagat
      301 tggtgcattg cgtcagactg aagtaatttt ttcagtgaga tagctcttta tattctaaaa
      361 accatactgg gattggctca gtggtttatg gttgatcaaa aaacttcctt aatgaagggg
      421 gtattgattt ttccctattg gttttataag aaatcgctga taccctagag ttgcatgaaa
      481 cctcagtatc tcgtgtaagc aatcaaattt ctaagtttnn nnnnnttttc taaagagtcc
      541 agggtgagtg ttaacacgaa gcgctgtagc gaagtatcgg gagttgctac gcatccccac
      601 catgcatttg cgtagacctt aatcatggag gtttgtcatg catttaagaa ttaatagccg
      661 tcatcttgaa gttactccag caatccgcac acatttagaa ttccgtcttg agcgcattcg
      721 taagcatttt gatcatgtga tcgatgcatc tgcatttctg atggtggata aggccaaaga
      781 gaagagcctg cgccagaccg ctgaacttac tctgcaccta aaaggcaagg aactctttgc
      841 ccaggctcac catgaggacc tgtaccacgc agtggatgcg gtgattgata aactagaccg
      901 acaattggtc aagcacaaag aaattatcca agatcaccac catgataaaa ataaggtgca
      961 aagtatttct caatagagac ctataatcta aggtcatgag cgtactcact gaccttttta
     1021 ctgctgatgg cattaatctc aatggtttgg ctaaatccaa agtcgaagcc tttgagattg
     1081 ccagtcagta ctttgccaaa aaactcgata tccccgctga aaccgtatta ggatttctgg
     1141 aagctcggga agcattagga agcaccggtt taggtgaggg tgtcgcaatt ccccatggtc
     1201 gcattaaggg gcttaaacag ccggctgctg ccttctttcg cttaagtagt cccattgagt
     1261 tcaatgcccc tgacggtcaa gctgtctctt taatcatttt tttattggtt ccagaaaaag
     1321 caacnnnnnn nnnnnnnnnn nnnngttcgc gaacaattac tcacagagcc agatccacaa
     1381 aaagtccaac aactcatttg ccattgggag gcataaaaat tgagtcaaca gcttttactc
     1441 gaaggaataa cagcacaaca aatctttgat gacaatatag ctgacttaaa actgtcttgg
     1501 attagtgggc ttgaaggtgc agatcgcaca tttgattcac aagctgtcaa agcagcggcg
     1561 gcctcttctg atctggtggg ccacttaaat atgattcatc ccagccgaat tcaaatcttc
     1621 ggcctccagg aagtggatta ccacgccaaa ctttcaagca gtgagcgaca agcacaaatg
     1681 agctcactaa ttactagcaa tcctccatgc atcatcgttg ccgatggctg taaaccggat
     1741 gaagagttac agctgtattg tcaacggtca tcgacccctt tgttctcaac gagcacttcg
     1801 gcagcagagg tgattgacca tcttcggttt tatctaacca agatcggggc tccgtgcagc
     1861 accatgcatg gtgtgtttat ggatatatta ggtcttggag ttttaattac tggtgaatct
     1921 ggtcttggta aaagtgagct aggtctagag ttaatttcta gaggtcatgg cttggtggca
     1981 gatgatgccg ttgatttcac acgcctcgga ccagactata ttgaaggtcg ttgcccagaa
     2041 attctgcgtg atcttttgga ggttcgtggc ttgggtctat tggatattcg gacgatattt
     2101 ggtgaaacgg ctgttcggcg aaaacttaag ttgcgcctta ttgttcagct tgttcgtcgt
     2161 aatgatggtg aatttgaacg actgccgatt gaatcacagt tcattaatgt gcttgatgtt
     2221 ccaattcgca ctgtaaagat tcaggtggca gctggacgaa atctggccgt actggttgag
     2281 gcagctgtcc gcaacaccat acttcaactt cgtggcattg atactctaaa agatttcatt
     2341 gagcgtcaac gcaatcaaat gagcttggat agcgaaaata ataagaacca gggtcgttta
     2401 ctctaagatg cgaatccaac tgattaccgg catttctggt tcaggtaaat ctgttgccct
     2461 acgagcctat gaggatgcag gttatgactg tattgataat cttcccgttt ccctcatcga
     2521 acaattggtc gcaacattag aatcggaaaa ttgcaagcaa attgcaattg ccattgatgc
     2581 acgtcgcggt gaatctattt cacaagtacc agccctcttg caaaaattac gcgcacatca
     2641 tgaggttaat atccttttct taaatgcaga taccaataca ttggtacaac gtttttctga
     2701 aacgcgtcgc cgccacccgc tctcgaatcc aaatgaatcc actaccttga ttgatgtgat
     2761 cgagcaagag cgaacattac ttgccccatt agccgaccag gcccaacaga ttgatactgg
     2821 tcatattcct gcgcacacct tacgcagctg gatttccgat catctgaaag agaagccaat
     2881 aggtcttgca gtcatttttg aatcctttgc ctttaaaaaa ggggtgccta gcgaagccga
     2941 tattgtgttt gatctgcgtt gcctaccgaa tccccactat gaaccactgt tacgcgatct
     3001 cagtggaaaa gatgcgcctg ttgctgagta tctcagccgg ttcccagaag ttacacaaat
     3061 gatggaggac attaaatcct atttagaaaa gtggttaccg cgctatttac atgatgggcg
     3121 tagttattta actgttgcca ttgggtgtac cggcggtcaa caccgatcag tgtatttggt
     3181 tcaacaactc aaaaagtatt tttctgaaaa tcagatgttt aacgatattt attatctaga
     3241 gcgtcatcgc gagctggatt ctaaaaaaac acggattggc tgaggtaagc caatttcgac
     3301 tatgtttgct cgtttaatcc agatcagatt aggatggctt attgtccatt ttcgttttaa
     3361 ggatacaagg tagggataca tctgcaaacg acgatggcta aatacatgct gaattactga
     3421 actggtctgt actgacataa tttgcttgaa gtcaatatca agggctttgt cgcccagcag
     3481 tgttttactt agtatttttc gagcattgcg cacagtcatt aaggcgatat ctttgctttg
     3541 cggctcccat ggtaattcga ttaaggaata taagccgccc caaatcgctt ttggcgctct
     3601 tcgttccagc aaaataaatt ctccgctctg tattaaaaag atagaggtct gaaagcttgg
     3661 agaagcagac ttttttttct ttgctggaat cttatctacg cgattagttt ggtaagcaag
     3721 gcagttattc atcatggggc atcgcgcctc attgctaata caccttgctt gctttggagt
     3781 gcaccatgtg gccccaaagt ccatcaatgc ctgtgtatag attggcattt ttattgatgc
     3841 ttttggtaat tgtgcttgcg cccttaccca aagttgctta attgtttctc gatcttgaag
     3901 atcaccctct attccatgaa aacgactaag aacccgctta acattcgcat ccaagatcgg
     3961 ggcatggaca tcataggcga atgctgcgat cgctccagca gttgatcgcc caataccagg
     4021 taagctttca agcgcatcga gatcccgagg aaattttcct gaatgctcac tcataatttt
     4081 ttgagcacaa cgatgaagat tgcgcgctcg tgagtagtag cctaaaccac tccataaggc
     4141 catcacatca tcgattggtg cgttggctaa ggagctcaag gttggaaagc gcttcataaa
     4201 aagtagataa cgctcactga ctgtgcttac ttgagtttgt tgcaacatga tttctgaaac
     4261 ccatatcgca tacgggtcgc gcttgttctg ccaaggcatg ccaactcgcc catgctttgc
     4321 gtgccaagaa attaatgcgt ttgcaaagat aaatgccatc gattaacgct tttgacagac
     4381 tgggcaaaaa tatgtggagc gttgcccttg cacaatttgc tgtatcggtg ttttgcacac
     4441 acgacagggt tcgcctgctc ggtcatagac tttagtttgc atcatgaaat agcctggctc
     4501 tccgtgtgca tccacaaaat cacgcaagct acttccaccc gctttaatcg ctttacttaa
     4561 aacatttcga acagaatcag ctagacgagc gcattggatt ttcgttaact tcccagccgg
     4621 gagtcgcgga tgaatgcccg cttcaaataa tgattcagag caataaatat tcccgacacc
     4681 tacaaccgcc tgaccagcta agagaaaaga ttttatggcg atggttcgtt ttcgagagaa
     4741 ttgatacagg cggatagccc ccaattcacc cgcaaactct gaagcaaggg gttctggtcc
     4801 taatttcttt attaatgggt gttcctctaa aggccccttt gaaaaaggat gccacaggac
     4861 agcgccaaac ttacggggat catgaagccg cagtccaatg cgaccaaagt ggattaagac
     4921 gcggtcatgc ggtttaagag gatcggtcat tggtaagact cgcaataccc cagtcattcc
     4981 cagatggatc aataaatagc cagaatccat cttgagcaat agatactttc ctcttcgctc
     5041 aatcgtatct aaacgttgtc caattaatac ctttggcaat gatttaggga ctggccaacg
     5101 caaacgacca tccacaatct ttaccccggt taccgaccgc ccctcaatat gggcttggat
     5161 tcctaaacga gtgacttcta cctctggcag ttctggcata aatgaattgt agtttcgggg
     5221 attagaatgt gcttatgtac ctcaatttca tgcaaaaaca agcaaacagg cttggtttac
     5281 tctgcctttt tatgctgttg tttgccttct tgagtagtca gcatgcgtat gcccaaaagc
     5341 cacccaaggg cgcgactagc gaacaagtat ttgagatatt ggcctcagaa atcgccctgc
     5401 aacgcggcga ggctggcttg gcttaccaaa cctatttaag ccttgctcga caaaccggtg
     5461 aagcggatct tgcgcaacga gcaatggaaa ttgcgattgc agccaatgct cctgatttgg
     5521 cactagcggc tgctcaaacc tgggatgagc tttctaaacc atcccaaact agacctaaag
     5581 aggtcttagt aaccctattg atgctcaatc aacgttggtc agagtctgta aagcctgcgg
     5641 tggctcttct caatcaacag cccttgagtg agcgcgagaa aacactcaag cagtggcaag
     5701 gcttaattgg tagagccatg gatgagagcg ccgccatgct ggcctattac aagattattt
     5761 cagccttagt cccactgccc agtagccccg atgttttgta tagctatgca ttagctgccg
     5821 agaaagctgg tcagtttgag gtcatggaaa aaactttacg gatcattctc aaaaaagatc
     5881 ctaataatgt taattcttta aatgccctcg gttactcctt ggccgatcgc aacataaaac
     5941 tcactgaagc ctatgacctc atcttgaagg ctcacaaact ttctcccgac gatgctttca
     6001 ttcttgatag cttgggatgg gtcaactttc gtttgggtaa aacatcgctn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggcgccaagg agaactaatt gatgcaaata
     6181 atgcaaccct caaagagact ataagacgcc tcaagccaga ctggattatg tccttggaca
     6241 aattagccag tcaatgggac ggacgctttg ctgtgaagat gaatgatcgc cccaccaaca
     6301 atacacaagg tggaagcggt agctttacgc tcagcaaaac cggttttagc gatacccttg
     6361 agatccgaag ccctatgggt ggagcgattg ccaagattgt gattaatcca ggggaagcca
     6421 ttttagagcg cgatgggaaa aaggtaatgg caattgatgc tgataccctc attcaaaata
     6481 cgcttggact gcccttgcct gcccgcggtt tatcgaattg gctcaatggt caagttcgtc
     6541 ccggtagtgc ggctcgatta gtgcgcgacg aacgcggtca agtcaaaaat attacccaag
     6601 atggctggga tctagcctac aagtggagtg atgctcaaaa aattgagcgt cttaatatga
     6661 ctcgtaacac gaataccggc actatcgatg tacgtttgat atttgatcaa gtgaatgact
     6721 aaaaaaattg agctgtacgc tccagccaaa attaatttat ttcttcatat cattggccag
     6781 cgagacgacg gctaccatca gttgcaatct gtatttcagc tcatcgactg gtatgacact
     6841 gtgtctcttg agcttattga agccgatgaa attgttaggc cagttggggc agatggggtt
     6901 aatgcagaaa acgatttagt tgttcgtgcg gcgcgtctat taaaaaagta tgcatcttac
     6961 ccatatggcg taaagattca tctagaaaaa aatattccca tgggtgctgg tttaggtggn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnaatt gggtgctgat atccctttct ttttatttgg
     7141 acaaaacgca tttgttgagg gcattggtga gcaattaaac aggcttgatc tacccgagga
     7201 acaattttta gtgattttcc cagggcaatc ggttgccacc aaagacgttt tccgagatga
     7261 cacattgacc cgcaatcatg ctccgattac aatttcagac tttcttgcta gctcctggaa
     7321 ggatcccaaa tttgggaatg atcttcaacc agtggcaagc aagatctgcc ctgaagtaaa
     7381 ccgagcatta gattggatcg cacaacaagc tccggattct gtaaaacgaa tgacgggatc
     7441 gggtagctgt gtgtttgccg ctatcccaaa aacgattagc gcacctgagg tcgatcgcat
     7501 gctgcaaaaa cttccttctg ggtgggtggg tcggcttgtt cggggattaa aacaaaatcc
     7561 cgcttacaat tcggtttaat cagtatgacg ctacagggga gtcgccaagt tggtcaaggc
     7621 accggatttt gattccggca tgcgagggtt cgagtccttc ctcccctgcc atcgttatga
     7681 ttgataacta acatttagcc aagaccatga cctctgacgc gctcactatt tttactggca
     7741 acgccaatcc cgccttagcc gaggcagtag ccaaacaaat gaagttgcag cttggcaaag
     7801 cctttgtagg gcgcttttca gatggcgaga ttcaggttga aattcaagag aatgtgcgtg
     7861 gcaagaacgt tgttgtaatc cagtcaacct gcgttcctac aaatgacaat ctgatggagc
     7921 tgatgatcat gattgatgcc ctgaaacggg cctcagcaaa ccgtataaca gccgttattc
     7981 cctattttgg ctatgccaga caggatcgga ggcctcgatc ggcccgcgtt gctatatcgg
     8041 cccgcattgt ggccaatatg ctgcaatctg ttgctggggt tgaaagggtt ctaaccatgg
     8101 atttacatgc tgatcagatc caagggtttt tcgatatccc tgtagataat atatatgcct
     8161 cacccgttct tttaggagat ttgcgggcta agaatcaggc tgatttgacc gtggtatctc
     8221 ccgatattgg gggggttgtt cgtgcccgcg cctttgctaa acagctaagc tgcgatttgg
     8281 ccattattga caagcgccgc ccaaagccaa atgtttctga ggtaatgcat ttaatcggtg
     8341 aggtcgaaaa ccgtcattgc gtcatcatgg acgacattat tgataccggg ggaaccctgt
     8401 gcaaggctgc cgaggtcttg aaagaacggg gcgctaagag cgtaaccgcc tattgcactc
     8461 accctgtctt atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnatt gccccccttc tggcagaaac catttcccgc atcacaaaag gtgactctgt
     8641 gatgtcgctt tttgctgact aagctctctg tagagggtct tttagaggtt ttttagccct
     8701 ttcgggctat aatttaaggc tttccgtttg gtcgcgaacg gaaatttaac cttcttagga
     8761 gtcatcatga aagtagttgc atttgaaaga agcgtacagg gcactggtgc gagccgccgc
     8821 ctgcgcaatt ctgggaaaac ccccggaatt atctatggcg gaaagcaaga atctccctgc
     8881 gcaatcgagt tggaccataa cgccctattt cacgctttgc gtaaagaggc cttccattcc
     8941 tccattctgg acatcgaact caacggtaag gcacaaaagg ccttattgcg tgactatcag
     9001 atgcatccct tcaagcctct cgtattacac gtcgatttcc aacgtgtttc tgcgacagaa
     9061 aaaattcata tgcgcgttcc gttgcacttt aaaggcgcag aggaatctga agctgttaag
     9121 tttggtggtg cggtagttag ccatattgcc aatgaattag aaattacgtg tttaccggct
     9181 gacctgcccg agttcttgga ggttgacctt agcaagattg ctgttggtca atcgatccat
     9241 gcaaaagagg tatctttgcc aaaaggcgtt gccctagtct tgcatattga acaagaaaat
     9301 cctgttattg ccaatgcccg tatcccaacc gttaaggctg aaccagagcc aagcgcagca
     9361 cctacagcac ctgtggctga tgccgctgct ggagataagg aagcaaaaga taaagaagca
     9421 aagagctaat ctctcttatt ctgatgtctt gaagaacccg ccctgtgcgg gttttttatt
     9481 gatcaatccg actcgtttat agtttgatga ttgctataaa taatgatctg cattctcata
     9541 tgattcgctt aatcgttggt cttggtaacc ctggtgcaaa gcacgaatcg gatcgtcata
     9601 atgccggatt ttggtttatt gatcgcttag cgagtcaaca caaacagctc cttcagcctg
     9661 aaaaacgctt tcttggcaaa gcagcaaaga ttcgggtgga agggcaggat attcacttat
     9721 taacacccga tacctatatg aacttaagcg gggaatcggt tgggccgctg tgtcgctttc
     9781 ataaaattac accccaagag gtcttggttg ttcatgacga gctcgatcta aaacctggta
     9841 tggctcggct caaacagggt ggtggcaatg gtggtcataa tggtctcaaa gatattcagt
     9901 cccatttatc aagcccacaa ttttggcgcc tacgctttgg gatcggccat ccccgtgatc
     9961 ttcctggcga taaagcaaag atggatgtgg ctgattatgt attaaagaag cctagcagcg
    10021 aggaacaaag taagattgat caagcaatcg ataaagcctt aagaacactt cctttattta
    10081 taaaaggtga tgttcaaaat gcgatgcagg ctatccacgg gcccgactaa ttaaaccgcg
    10141 gcctttttaa tcttggtcag cgtgtgatat ttactcatta gctgttcttg agtttctgaa
    10201 taatttggat ttaatggaat gcagtctact gggcagacct gacgacattg gggtacatcg
    10261 aaatgcccaa cacactcggt gcatttattt ggatcaatct catagatttc aaggcccatg
    10321 taaatggcat cattgggaca ctctggctca cagacatcgc aattaatgca ctcatctgta
    10381 atcattaaag ccatggttta aacctttagt gtacgaacca aaccagaata gcttattttg
    10441 tcttggtttg tgcgttcttt tgatgtaacc atttttctac tgagggaaac acaaatttac
    10501 tgacgtcacc gcccattaat gcaatctcac gcacaaatgt tcctgaaata aattggtact
    10561 gatccgatgg cgttaagaac aaggtttcta catcgggtaa caagtatcgg ttcattcctg
    10621 ccatctgaaa ctcatactca aaatcagaaa ctgcacgtaa gccccgcaca atcacgcgcg
    10681 cattgtgctc tcttgcaaaa tctttcagca gtccgcagaa cccagcaatt tctacattgg
    10741 gatagtgccc caatacctct tttgcaattt caatacgctc ttctaaggta aaaatcggct
    10801 ttttacttcg actatcagca acaccgacaa taagcttcgt aaaaatacta gaagcgcgac
    10861 gcacgagatc ttcgtgtcca cgagtaaacg ggtcaaaagt tcctgggtaa acggcaatgg
    10921 tcataagaca cccttaataa tctgaatata taagagttta gcccgaagtg cgcctgaata
    10981 atgctgcttt tgtttgtgtt gtctgcattt gtcttgaaca ggcccaatta ggcattaaca
    11041 actctaactc taaaagatct tgagtagtcg gacattcaat ataaattccg cccccagagt
    11101 ggtcatcaca aatacgggca gcttccacga ctgcctgctt taagagcgca ggctggttaa
    11161 agggtgggtc aagaaaaatc aaatgatagg ctgcggtgtt cgcttggccc atatggataa
    11221 tcccatctcc ctgcatgacc tttaaatttc cttgaattgg ggaagataac aaaagctgat
    11281 attgggattg cagctgcgca acgatctttt tatctttttc gattagaacg acttcttttg
    11341 catcgcgtga cgctgactca aaaccaagag cacctgatcc agcaaaagca tcaagaactt
    11401 taaggccact taggtcttga cctaaccagt taaaaagagt ttctcggaca cgatcagagc
    11461 tagggcgcaa accctcttga gataagactg aaatttgtcg actacgccag accccaccaa
    11521 taatacgaat ccgattggga tggcccgaca attactgcgc ccctccaacc agaatgctaa
    11581 tcatgcgccc catatctaaa tggcgctgaa acgctgcacg tacatcttct ttttttactg
    11641 cagccacttg ctgagtccaa acatccaaag tattaagtgg caattgattc caagtaatgg
    11701 cagacaagtt atccaataat ttacgattac tatctaaacg tagtggaaat ccattcatta
    11761 aatttgactt ggccgctgcc aactctgaat cactaggccc ctctttaata aagcgagcga
    11821 ttgtttcttg taaaactact agggcttgat tggcttgatc gttgcgcgtc tgcaagcccg
    11881 cctcaaaact accagtttca cgaccaggct gaaaatagct aaacacacta taggcataac
    11941 ctcgcttatc acgaacctct ccaaccaatc tagagacaaa gccaccaccc cctaatacat
    12001 aattaccaac caggagtgga aaatagtcag gattactccg cgtaatagaa ctcattccta
    12061 atgcaatatg agtttgctgc gtctgaaacg gaatacgaat tagtcgatcg gcctcaggtg
    12121 caagcggtga aggaataaaa gggggtaatg ctgggattgc tgggccctgc tgtgggagct
    12181 gagcgattaa tttctcagcc acttgtttag ctgtttttac atctaaatca cccaccatat
    12241 tgataatcat gcgatcttgt cgatagaaat ttcgatggaa tcgttttaga tcatctgcgt
    12301 caatggaagc aatcgatagc aagctgggtg actccgctaa gggataatcc cggaataact
    12361 ccttacgaaa gcgcttttgc attactactt caggctttgt atccccttct gcaattgccg
    12421 aggaaatccg ttgtttctcc cgagcaacaa tctgtacatc aaaaattggt tttgagatag
    12481 cagcacttaa taaatcaaga acaggattaa atacatctgg cttactcaaa gacctggctc
    12541 gcacaatagt gcgctcgcca cttgcggatg cgctaatgct tgctcctaga tcagcaatag
    12601 catcagcttg cattgcctca tttaaaaggc tgccgttcca ttcaattcct cgggttaata
    12661 gagctgcggt taagcctgct aggccacttt ttccctttgg gtcgtaacga ctaccagcat
    12721 cgacactaat ctcaatatca atcattggaa ttgtggtggc ctgaactaaa taagctttac
    12781 tgccattact taatgcgatc tcttgaatgg gtaagacggc atatgcctta ccccatatac
    12841 cgcaccccag tgcgataaaa ataaggaggt aacgcttaat ataaatctga acgatataca
    12901 tcatattttc tcaatgccgc aaattgctag gaagggaggg aataggtttc ggatttgccg
    12961 atacggttct tgcttgtggt tctagggtga caatcgttag accgtcaaca atgaaatatc
    13021 gcttaaccgc tgccttgatt tgctctgact ggatttgctg aatgcgctca atgattcgat
    13081 ccaaatcttt ccatgagaca tgagcaatct cagcactacc aatttccatt gcttgagcaa
    13141 aaatagagtc acgcttatag atttgagagg ctaaaagttg agccttaatc ctttttagct
    13201 ctgcctctgt gatcccattc atggcaatct cttgtatagt ttgtaagata cctttttcca
    13261 gatcagaaac tgactgttct ttagccacag ttgcactaat cataaaaagc tctggtccgc
    13321 ggctaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga ttgagtcggg
    13381 agttatcatg gccactgagt gctgcagaca agacctcaag cgcataggga tctgcatcat
    13441 ccattttctt tgggtctaat ttaggaactt tccaccccat cattatttgt gggctatcgg
    13501 ccagcgcttt gagattagcg cgtttttctc ctttttgttc gggctctact tgcggcttac
    13561 gttctggtaa ttgctttgca gaaatcggtc catagtattt ttctacaagc gctctcacct
    13621 ctaagggttt cacatcaccc acgaccacaa caattgcatt attgggggca taccaatccc
    13681 gataccactc cctcgcatca ttgggcttca tgttttgtaa atcgttcatc cagccaataa
    13741 tgggatggcg ataaggagag cttgtaaagg cggtggccat catcaattcc cgcatcaagc
    13801 catttgggct gtcatcggta cgtagacgtc tctcctccat cacaacctga atttccttta
    13861 agaactcttc atctgaaaac ttcagattgg ccatacgatc agcctctagt cgcatgacat
    13921 cggcaagttt tgatttttca atctgttgaa aatatcccgt gaaatcacgg gcagtgaatg
    13981 cgttctcacg ccccccgact gctgcaacca ttctggaaaa atctcccgat ttaacctttt
    14041 ctgttccctt aaacatcatg tgctcaagca cgtgcgcgac gccggtcttg ccattaagct
    14101 cgtccatcga tcctgcgcga taccaaacca tatgagccac tgtgggtgca cgacgatcct
    14161 ctttcactac taaacgtaag ccgtttttaa attgatactc gtgtgtatct atcttattgg
    14221 cccacgcccc tagcggtaag catgcactga cgcataagcc caacaggatc agaatcctca
    14281 gggtggataa gcaaacgaat ctaggtgtgt ttagagtgac tatcatctca ttgttgacgt
    14341 ggtcttgaac ttgttaagat ctacctaaag gattgtatcg ataatgtttg gtttacgtaa
    14401 aacgcttggt tccctatttg gtcttcatcc aattgatgag tcctggtttg agcaattaga
    14461 agaaaccctt ctaaaggcag atgttgggat tgcgaccacc aacgtactta ttgctagcct
    14521 tcgtaaacta gctaaatccc aattgatcca aaatgctgag gatttaaaag ggttattggt
    14581 tcaggaaatt agccgtcttt tagctcctct tgaaaatcct caaaaccctc ttttattaag
    14641 ccagtcaaac cacaaacctg aaatctggct aatcgttggg gttaatggtg caggtaaaac
    14701 aacctctatc ggcaaacttt gtcatcactt tcaagtgcaa ggtaaatctg ttttattggc
    14761 tgctggcgat accttcagag ctgcggcgcg caatcaactc aaggaatggg gggagcgcaa
    14821 ccatgtccaa gtcttaagcc aagaaagtgg tgacgcggca gccgttgcgc acgacgctat
    14881 acacgctgcg atttctcgcc aaatcgatgt tctgtttatt gatacggctg ggcgcttagc
    14941 gacccaagga aatctgatgg aagagctcaa aaaggtaaaa cgggtgatct ccaaggtggt
    15001 tccagatgcg ccccatcaaa ttgtcctcgt tctagatggc aatactggtc agaatggcat
    15061 tgcacaagtt ctggcattta ggaatgcaat tggtcttcag gggattattg tcaccaaatt
    15121 ggatggtact gctaagggtg gcgttatttg tgcgcttagc aaagcccttg agaccccccc
    15181 agaaacgggc acccccgcct tcaaaacctt tgtctatgcc ctgggtaaag gggagggtct
    15241 gtctgatcta gagcccttta atgcagaagc ctatgctaaa gagcttgtag aataaccata
    15301 ttaatcaagg tcttataaat tgatagtgag attagcactc tccttatagg agtgctagaa
    15361 ttaacatatt gcaatcaaac taatcaataa atcgatgact ttgaatacag ccttaaaacc
    15421 tattaatcgc aatacttcgg caggcggtct ggctatgcct ttgcctgcgc ttggcgttgg
    15481 aacgattgac gcctatttag cgcatatcaa ccgcttacca atgctgagcg ctgctcaaga
    15541 atttactctt gcgcaggagt atcgtcgttc tgaaaattta gatgcggctc gtcaactggt
    15601 tctctcacat ttgcgcctag tggcctctgt tgcacgtcaa taccttggct acggaattcc
    15661 tcatgccgac ctcattcaag agggcaatat tggtttaatg aaagcagtga agcgctatga
    15721 tcccaatcaa ggcgtgcgct tagtctccta tgcgattcat tggatcaaag cggagattca
    15781 tgagtacatc ctaaaaaact ggcgcttagt taaagtggcg actacaaaag cgcaacgcaa
    15841 actattcttt aatttacgaa gtaacaaact ttcagcaaat gctcttactg attctgaaat
    15901 tgagtcactt gctaaggctc ttgatgttcg cggtgctgat gtcaaagaaa tggaaatgcg
    15961 ccttgcaggt ggcgacatct ctattgatgg cgacgatcag gatgaagacc aatttgcacc
    16021 cattcagtgg cttgcggatg agcgccatga gcccagcgct agactttctc aaaatgatca
    16081 atttgcattg cagggcccca agctactgca agctttaaat accctagatg ttcgcagtcg
    16141 ggagattgtg caatctcgtt ggctcaatat ggatgcagat ggccaaggct gcaaaacctt
    16201 gcaagacctt gcacaagaat atggcatctc tgcagaacgg gttcgtcaaa ttgaagtagc
    16261 tgcattcaag aaattacgac tcgttcttaa agacgaagaa tccttactac attaattact
    16321 ttagaagtat ttttaaatca tgggcaatgg gctctacccc ctgcccatgc tttatataaa
    16381 ggcgaaggtt tccagcagga tcaaacacat aactacctgc tgtatggtca atcgtatagt
    16441 ttttaggatt gctgccaggc actttgctgt agtaagcctt aaagtctttt gttatcttca
    16501 ttagcgcagc ttcatctgca ggtctcaaac caataaaacg gggatcaaat gcgggcgggt
    16561 actgcgccat taactcagcg gtatcccgtt ctggatcgac cgtaataaaa ataacctgca
    16621 cgcgatcggc aagtggcccc aaatccttca tgactccctg catctcaatc aaagttgagg
    16681 ggcaaacatc ggggcaatgg gtatatccaa agaataggac aacggcctta cccttgaagt
    16741 cggctagtgt tttatctttg ccttgagagt caagcaacac aaagttgttt ccaaacgact
    16801 tgctgccggt aatatcaaca tttttaaaac tgggctgtga gcaagccgcc aataaagtaa
    16861 gcattgctaa tgtaagtagt ttcttcatag agctaaagat agtgatcaat caagattgca
    16921 gcaaataata gtgctaaata gctgatggag tatttaaagg tcttacgcgc caactgatcg
    16981 ctatagtttt gataaagacg aatcgcatag taaataaata taccgctcaa aataatcgct
    17041 gataccaaat aaaaaatacc gctcattcca tagatatatg gcaggatcga tgcagctaac
    17101 aaaattaagg tgtaaagcaa aatatttaaa agagtaaatt tctcaccatg cgtcacgggt
    17161 aacattggta atcctgcttg tacataatct tcacgacggt acagagcaag tgcccaaaaa
    17221 tgaggtggcg tccatacaaa aataatcagc acgagtagcc aagcttcggc tgagagttga
    17281 ttggtcacag caacccagcc caaggcgggg ggcatcgctc ctgaaaggcc gccaattaca
    17341 atgttttgtg gagttgcggg ctttaggtac caggtataga taaccgcata gccaacaaag
    17401 gtggcaaagg taagccacat cgttaagggg ttggtataaa accatagaac gaccatacca
    17461 gcgccaccaa gcccgcatga aaaaataaca atttggaggg gggtaatttc tccggtagca
    17521 gatgggcgcc atgctgtacg ccgcatcttc gcatcaacag cttgctcaat taagcagttc
    17581 actgcaaacg cagcacctgc tagcaaccat ataccgatgg aacctccaat caggatgggc
    17641 cacggaacca tgcccggggt tgccaaaaac ataccgataa tggcacaaaa gactgcaagt
    17701 tgagtaaccc gtggttttgt taaaacccaa tactgacgcc aacggggcat tggtgggcgc
    17761 tcaaaagacg aagtgctcac gatgacctct gcccatccac atcacgaccc gagaaaaaag
    17821 aacagtcact tcctgcactc agtcgtacta agcaaaataa gatagccgct gctccaccnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 agaatagcct tgccccaaaa agaaatattt gattggctat ggcgtaatgc tttgattgca
    18001 agcaagagca aaacaatgga tgcaaaaatg gctccaacac gatgagtcca gtgtatggtt
    18061 accaaagccg tcatcggaat cacctcacct gattttgctt gaccaagctc acgccataaa
    18121 taaaatgcgt tttgccaatc catggtggga taccatgcgc ccatacaggt tgggaaatca
    18181 gggcaagcca atacagcata gtttgtgcta acccatgctc ccaaaaatat ttgaataagt
    18241 aaagtaatcg tagcgaatgc aatccatgta atgggtaatg atttaccttg agcctttgaa
    18301 gatattattt tgctatcgtt acgctgagca aaccaaacca tactagaaaa taaaattagg
    18361 gccaagatca aatgcatact cacaataatg ggttgcaact tcaaggttac cgtccaagca
    18421 ccgaaggcgc cctgtaagca caccaagacc agtagaaata gactgccttg aatggctaat
    18481 nnnnnnnnnn nnnnnnnnna cccctacact cattgctaaa tagcgatgga tcatttctac
    18541 ccaagcctta aatacggtta caggtcctgt gggcatcgct acctcagcgg cgcggatctc
    18601 ctccatcgcc ctccagggat ttgaggtgcc ataacaacct ggccaatccg gacagcctaa
    18661 acctgaatca ctcaaacgcg taaatgcgcc aaataaaatg aggtcgaagg ttaaaaagac
    18721 gactgcccaa tttagtttct gaaaaatatc taagcctttt ctacgcgcca gataagccaa
    18781 aggtaagccg gcaaagacaa aggcaattcc agcaagctct aaaactaata gagccgattc
    18841 ggaaatcata ttttttcacc tttgcgattt agcttcagta gacgatctaa atctttacgc
    18901 attctgacaa aatcttttga ctctgcttta tcggggaaca ccatcatctt ttcgccactg
    18961 ggatctatca actgaatccc tctccctagg ttctgctgat ttaaccaggt caaccaggct
    19021 gcgccatttt ttggatctgg ggcaggaaca atgataaatc cagcagtttc tggatcgtag
    19081 gcctgtttga tatcagggtc tacgggcaag cgatcgctga ccacccaaac aagctgtacg
    19141 cggttcttct ctttccccaa tgcaagacgt acttgtctca tcagatataa gagctctaca
    19201 caagcatcat tccctttgcg acactcgctg gcggggcgtg ctactaaaag agtccacttt
    19261 ccgctaaagg aaatatccag ccagctggga ttaattgcct ggggaggctg tatcagagtc
    19321 ccatagtttg tttttccacc ctcaggctta aaaacataat aggctaagta ggatgcaatg
    19381 actggagcag cacaggccaa taaaagcaag atcatctgga ttcgaccccg tcgcgttctt
    19441 gcgtctaccg cttgccgatc cacttgactg gctggtatta gtaaatcttg atcattcact
    19501 ggcgtcttgt cccattaaaa cgatttatct ttattgcggt aacgcataaa accggttgta
    19561 aaccaaaaca cgagggccgc tgctgctaaa gcaaaccatt gaaaagcata agcataatgg
    19621 cgatcaactc caaccgttgc cggtggccaa tttcgtaata agccatcatt acgatctggg
    19681 tcattttgtc gtatcacaaa tgggagctgc gggtacttta actgcttttc ttcatatgca
    19741 agatcaagat tttgttgaat gcgtggacga tctttggagt ttggttggtc acccaactcc
    19801 aacacctttc cagcacccgc aaatgccact ccctctatcg tgaccctgcc tgtaggcgta
    19861 ttaatacttg gcaatatgag tcgatcctga ctattccgag gagcccaccc acggtttacc
    19921 cagatgacct gagtgaccgg tccgtccaag agaaatggca tcaatacata gaagccggaa
    19981 tgcgcctgac ttaggctacc gtggtttggt ttagggcgat tatctaacca tacaatctcg
    20041 ttggataaaa actgaccgcg cgcctggaca gcccgaaact cgacttctga taaagtccat
    20101 gccttggcat ttagatcaat ccgttcttta gcggccatcg tagcaatttg attagccaac
    20161 actatttttt gctccgcccg actcaattgc cagcgcccag caaggaggcc aatcgcaatc
    20221 actacgattg ccgccacagt cgctactcta cgctctacaa tgaagacaga aaaaaaactc
    20281 aagggctcgg taccgtaatg aaatggataa ttgttttggt cctgctgctt gttattggca
    20341 gcctaggatc agcgttgtac ttcatgatga aagataaggg caacagctcc aggatggttc
    20401 attctttaat gctacgaatc ggcttatcca ttgccttatt tataggcatt tggatcgcct
    20461 actactttgg cctaatccaa tccaccggtc taaaggttgc ccaataaaaa cggtagccga
    20521 agctaccgtt aatgactcac atccagtaaa tgacgatgta aaggcccagc caaacaacgt
    20581 caacaaagtg ccaataccac gcagcaccct caaaaccaaa atgattatcg gctgagaaat
    20641 ctccacggat taaacgacgc aagataatag ccagcatcag accacctaga aatacatgga
    20701 atccatgaaa accggtcaac ataaaaaatg tggagccgta gataccagag cttaacttga
    20761 gatttaaatc cgaataagca tgaatgtact cataagcctg aaaacctaag aagatcactc
    20821 ccagaagtac tgtagcaaat aagccataaa ttgcctgttg gcgatgccct tcacgtaatg
    20881 cgtgatgtgc ataggtgaca gtcaccccag agctcagtag cagcaaggta ttaattgtgg
    20941 ggatcggcca aggcccgatc gtactgaatt tctcgacgag gcctgttggg ccaagtgtgg
    21001 gccaagccgc tacaaagtct ggccacaata tcttattgtc cacatcgccc aaccaaggaa
    21061 ctgtaatgct gcgtgcgtag aacagggctg agaaaaaggc tgcgaagaac atgatctcag
    21121 agaatatgaa ccagctcatc gaccaacggt atgagatgtc aacgttaaca ccgttctttc
    21181 ccgtattgcc ttcagaaatt gcatctccaa accagccgta cagcacaacc aagatataaa
    21241 tgactgcgcc caaaaccaaa gggccgcccc acgataggtt attaacccat gcagacatac
    21301 caccaccaaa gccaagcaag ccgatgcttg ctaatacagg gtacttcgag ggacttggaa
    21361 caaaataata aggagtggag ttcgaagaca tcgtgctctt tctagcttaa aaattaattt
    21421 gatacaacta actgaacaat caacaaaaga attgccataa aaattatggc gcccaacaca
    21481 ccagcaataa tgatgtgaac aaaactaaga ctagcaacat cgttttgtag ttctgagttc
    21541 ttgcgtacgc ccaaaaaggc ccataaaaca gcacggatcg attgaagaag atttaatttc
    21601 tttttcacga tccactcgct tttgatggct gcgtcagtgc aactggtttt gctatgcccg
    21661 tttcaaaaaa agtatatgaa agcgtaattg tcttcacatc tttaggcaaa ttagggtcaa
    21721 caataaacac cactggcatt tggcggattt gatgggggtt aagagcctgc tcttgaaaac
    21781 agaaacattc aatctttgtg aaaaattcgg tagctgcctt tggggcataa ctgggaattg
    21841 cctgagcggc gatagcacgg tctttagtat taacaacctc atacacaatt tcatgtagct
    21901 cgccggggtg tacttcaaga aaattcttga ctggcttaaa cgcaaaaggt ccacggctat
    21961 ttgagtcaaa ctcaatcgtg atcttacgcg agtaatcgac ctgcgtgttt ccgggcttgc
    22021 ttgcgccatg cgcccgaaca ccataattat ttttgctcgt aatcacattg ataccagtga
    22081 cttcacacag tgctttatac aaaggcacca aggcataccc aaaaccaaac atcaagacag
    22141 ctagcaaaag gagcttgagt aagatttgtt tattaaggga gccgaggtta ctagccatag
    22201 atcaaccaaa tatccagtac ttcacgacaa ttcccaagaa aaacgccaac gcaattgata
    22261 acaacaccgc cgcgaggcgg cggttggtca tcattggatt ggatcgttgg gtcactaaca
    22321 actccgttta actaactttg ggtggttgtt caaaggtgtg aaatggtgct ggcgatggaa
    22381 tcgaccactc caaagtattg gcagtttccc aagggctatc tgacgccttc ttaccatggc
    22441 cacgataggc gggcaaaatg acaaacagta aaaaataaac ctgcatcaaa ccaaacccca
    22501 aaccaccaat cgaggcaatt aaattgaaat cggcaaactg tgttgggtag tctgcatacc
    22561 gacgtggcat gccagctaag cccaagaagt gcatggggaa gaaggttaaa ttaaagaaaa
    22621 acatagagcc ccagaaatgt acttttccac ggaactcatc tgccatgcga ccagtccatt
    22681 ttggacacca gtaataaaag cctgcaaaca ttgcaaatag agatcctgct actaatacgt
    22741 agtggaaatg ggcaacaacg taataggtat cttgcaatcc aatatcaatg ggtgccatgg
    22801 caagtactag tccagtaaat ccaccaattg tgaacacaaa gataaaacca atcgcccaca
    22861 gcatgggggt ctcaaaagtc attgaacctt tccacatggt ggctacccag ttaaatatct
    22921 tgactcccgt tggtacggcg atcaacatcg tggcgtacat gaagaaaagt tggccagtca
    22981 caggcatacc agtagcaaac atatgatgcg cccaaacaat gaaggataag atggcaatcg
    23041 acgatgtcgc ataaaccatt gaggcataac caaacagtct ctttcttgca aagacaggta
    23101 caacctcact gatgatgcca aaggctggca aaatcatgat gtacacctcg ggatggccga
    23161 agaaccagaa aatatgttgg tacattactg ggtcgccacc accggcagct gagaaaaagc
    23221 tggtaccaaa atgacgatcg gtgagaacca tggtgatggc gcccgctagt acgggcatca
    23281 cggcaattaa taaataggct gtaattaacc aagtccagca gaacattggc atcttcatga
    23341 gagacatgcc gggtgcgcgc atgttcaaaa tggtcacgat gatattaatc gagcccatga
    23401 tggaagaagc acctaacaaa tggagagcaa aaatagccat gtccatgcca gggcccattt
    23461 gtaaggtaag aggcgcatac agtgtccagc cacccgatgg tgctccacct ggaaccaaga
    23521 aggaaccaaa aagcaataaa gcagcaactg gcaaaatcca gaaactaaag ttattcatgc
    23581 gtgcgaatgc catatccgag gcaccaattt gcaatgggac catccagttt gcaaagccaa
    23641 cgaaggctgg catgatggcg ccaaagacca tcaccaaacc atgcatggtt gttaattgat
    23701 taaagaattc tggacgcagg tattgcaggc caggttggaa taactccaaa cggataccca
    23761 aggccattac accgccagcc aataagctca caaaggagaa gatgagatac atcgtcccaa
    23821 tatctttgtg attggtagca aataaccaac gtctccagcc atgcggatgg tcatgtgcat
    23881 gatcgtgagc gtgatcgtgg gttgtggata tgctgctcat actttgctcc agttcaatat
    23941 caatcgataa aaatagttca ttaaattact ttgcagcacg tgcggttgca aattcttggg
    24001 tctgaatcac gtcacctgtt ttgttacccc atgcattacg ggtataagtc atgaccgccg
    24061 ctaattcacc atcagaaatc acgccgcccc atttaggcat ggcacctttt ccattaatta
    24121 agatgttgta atttgcagcc tttggcccca tcactaactt actaccatct aaggctggga
    24181 aggctcccgc acccttacca tttgcttgat ggcaagcagc acagttgcta gcgtatacct
    24241 tagcaccacg atccatctgc tcagcaaggg tatatacctt acttggatca tccgctgtgg
    24301 cagccatttc ttttttcttc tgagctaccc attgggtgta ttcctcatca gagacaacct
    24361 tgaccacgat tggcataaac gcatgttgcg caccacaaag ctcagaacat tggccacgat
    24421 atgtgccaac cctatcagcc ttaaaccaag tatctcgaac aaatcctgga atagcatctt
    24481 gcttcacacc aaaagctgga actgcccacg catgaataac gtcgttagct gtggtgatca
    24541 tcctaatttt tttatttaca ggcaccacca tttcattatc aacctccatc aaatatgttg
    24601 ggctctttgg cgccaagtta ttgatggctt cacgtgaggt agacaaggtt gataaaaagc
    24661 taattccttc gccttcacct ttaatgtagt catagcccca cttccactgg tatccagtgg
    24721 ttttaattgt gatgtctgca ttagttgtgt ctttcatagc caccactgtt tttgtagcgg
    24781 gtaaggccat accaatcaca atgagcaaag gaattacagt ccaaataatt tcaaccgatg
    24841 tactctcatg aaatgaggct ggctgggctc cctttgattt gcggtgtttg aagatggaat
    24901 aaaacatcac gccaaataca ccaacaaaaa tgacgacaca aataatcatc atgaaccaat
    24961 gcagccaatg gatctcttcc attattttgg ttgctggtgc tgtgaaattt aattgattga
    25021 cctttgggcc ccctggcata tcttgcgctg caacaccaaa actccaaatg ctgaatccaa
    25081 gaaatagcca cgtttttgca aagaatttac taatgttcat ttttttctca aaatttaccg
    25141 aggcacccga gattaagtga agtgccatta ttgccattaa tcacagttta gacactcgat
    25201 tataggatga tttgcctgac aggcttcaat ttggaaaggg gctcgggata aaccctttct
    25261 acagataatt aaatgtgtgt acttacttat aaaagagatt tgcaactatt tacgtttaat
    25321 tgcatctggg cgaatatatg ctgttttccc atccgattta ctcagattct taccaagaac
    25381 ccatgcgtgt ccataaacaa tttctagggt cattcttttt gctctcagtg ccccgctcag
    25441 ggcatctnnn nnnggcatcc cttagataat tagtttccga ttcatattcc aaatacaaat
    25501 actccatgtc catcacggga tcagagaagc ctttttggac aagtgcatcc ccaatgtcat
    25561 gcatatccaa tgcccccggc aaactcttta tataagggct tgatttaagt gatgcgcgta
    25621 gttcttttcc agtatctggt cccaaatagc taaaaca
//
LOCUS       DDPQ01000029            7244 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_4414,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000029 DDPQ01000000
VERSION     DDPQ01000029.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_4414"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1749..1825
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2570..2615
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2792..2863
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5019..5090
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5779..5869
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6370..6442
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1791 a         1613 c         1736 g         1673 t
ORIGIN      
        1 agcccatatt ctcgctgatt gggctggagt ttgctggcac tcaggtaatt cgtttcactc
       61 aggcgcaaat agacctcagc taaattacct tgcgcagtga catagtctgg acgaagctta
      121 agagcaagct ctaaataatc acgtgcctct atccatttac cttgattggc cgatagtgcg
      181 gcaagattgt tatagggttc tggtagctcc ggaaactgtt gggtgatctc aatccaagtg
      241 ttcttggccg agacagtatc acgcaactct atttgtaggc gggctttcaa gaaccgtaat
      301 tgcacattgc gtggcgactt cgctaaggcg gcatcaatgg ctttaattgc atcagcaaac
      361 ttacgttctt tggtcagctt ctcaatatgg gttggaattg cgttcttaat caccggatca
      421 ggctcaataa tcaagaagga caaaaagggc accgagatgg tctccgctaa ttgatttgca
      481 gaggtctcgg gattttgatt ggcactcatt cggggtggat cggttggacc atacccacct
      541 aaatacggtt gcggagggac accagttgcc gctggaggga cacttggcga agtgctacac
      601 gctagtaggc ttaaagtacc aatcccaagc aaaagtctta gggtccaagt gaacaaggga
      661 gagcaggtct gggatggtgt cattacaaag agatcactaa atgggcgctt cattggatat
      721 actcagcagt ctaacaaatt catgctccat gtttccaaag tcttataagg ttcctcggtc
      781 taagtaagtt caatgacgct ctcaatctac aacaccctat cgcgcagcaa ggaaatcttt
      841 aaaccgattg tggctggtca ggtgcgcatg tatgtctgtg gaatgaccgt ctatgatttc
      901 tgtcacttag gtcatgcccg tgtcatgatc gtatttgata tggtgactcg ctggttgcga
      961 gctagcggct atcaggtgag ctacgtgcgc aatatcactg atattgatga caagatcatc
     1021 aagcgcgcgc ttgagaacaa agaaccgatt gctgtcttaa cgcaacgctt tatcgatgca
     1081 atgcacgagg atgccaaaat ccttgggctc ttgcctcccg atcacgagcc ccgtgctacc
     1141 cagtacattg cgcagatgca aggcatgatt ggtcgtctga ttgatcaaca aatggcttac
     1201 caagctgagg acggggatgt gaactacgcc gtgcgtcagt tcccaagcta tggcaagtta
     1261 tccggcaagt ccatcgatga gctcaatgcg ggtgagcgcg tcgccattgg cagcggtaaa
     1321 cgtgaccctc tagacttcgt cctctggaaa tccgccaaag ccgacgagcc cagtgataca
     1381 cgttggtcat ccccatgggg cgagggtagg cctggttggc atattgagtg ttctgcaatg
     1441 tcttgtgagt tactcgggca acattttgat attcatgggg gaggggctga tctgcagttt
     1501 cctcaccacg agaacgagat tgcccagagc gaaggcgctc tgtatggatc aaggccggtt
     1561 actaacccgt tcgtaaacta ctggatgcac aacgggcata tacgtgtgaa ccaagagaag
     1621 atgtcaaagt cattaggcaa cttcttctta atccgagatg tcttacaaca atttgaccct
     1681 gaggttgtgc gctttttcat gttgcgtgcg cattatcgaa gccctattaa ctactccgat
     1741 agtcagttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnngtcgc acaatgggta aagcgctttg gggatgctat
     1861 gaacgatgat ttcaacaccc ccgaggcgat cgcaagcctc tttgatttgg caagcgagat
     1921 taatcgagcc agcaacgcga acaccaagca agagctctcc cagactctca aacacttagc
     1981 aggcatgatt ggccttttgc aacgttctcc caatgagttt ttacaaagtg ggacaccgca
     2041 taccggcatg agccccgcac agattgagga gcagattgca gcgcgacatg ctgccaagct
     2101 caccaagaac tttaaagaag ccgatgcgat tcgggagcgc cttttaaatg atggcgttgt
     2161 cttagaagat aaacccagcg gcacagtatg gcgccgcgct taagaacgac atgccaaata
     2221 ccaaactagc tagtaaatct gttgctgtaa aaaaggtggg gcgcaagcct agtattagct
     2281 caccagacta ttggcatgag gcatgcgcag agttgatgaa gcaagatcgt attctgcgta
     2341 aattgatacc aaagtatggt gacggcatct taggaagtcg gggcgatgca tttacaacat
     2401 tggcgcgctc gatcgtgggg caacagatct cggtggctgc tgcccagtca gtctggaatc
     2461 gcactctaga gacccttgag catgaggtta ccccggcaca ggttcttaag accaaacaag
     2521 aggccttgcg cgcatcgggt ttgtcgaccc gcaaggtcga gtacattcgn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcat taaagacctc agcgcaatcc
     2641 gggggatagg tcgctggacc gcagagatgt tcctgatctt taatctcatg cgtcccaatg
     2701 tgctgcctct tgacgatatt ggcctgattc gggcgatctc gattaattat ttcagcgggg
     2761 aacccgtaag ccgtcatgag gcccgcgagg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncattccc caaaagacat
     2881 aaaattaggc tatgaagacc actttcctag actttgaaca gtcggttgcc gagttagaga
     2941 ccaaaatcga ggaactgcgc tttgtgcagg acgagtcctc ggtcgacatc tcggatgaac
     3001 tcaagacgct ctctgagaag agccaacagc tcaccaaaga gctctacgag aacctgagcc
     3061 catggcaggt atctcaagtc gcgcgtcatc cacaacgccc ctacaccttg gattacatcg
     3121 cagctttatt tacggacttt catgagttgc acggcgaccg tgtttttgcc gatgatcaat
     3181 ccatgatttg tggcttagcg cgttttgatg gaaaaccttg catagtgatt gggcatcaaa
     3241 agggtagaga tactaaagaa cgagcgctac gtaactttgg tatgagtcgt cctgagggat
     3301 accgtaaggc catgcgcatc atgcgacttg ctgagaagtt ccatctccca gtctttacct
     3361 ttgtagatac ccctggagca tttccaggga ttgatgccga agagcgcaat caatccgaag
     3421 cgattgggca caacttatat acccaagcag aactcaaggt acccatcatc accaccatca
     3481 ttggcgaagg tggctcgggt ggcgccttag caattgcgat gggcgatatg gtgatgatgt
     3541 tgcaatactc cacgtattca gtaatctcgc cagagggctg cgcctcgatc ttatggaaga
     3601 ccgctgagaa ggccccagac gctgcagagc aactagcatt gaccgcacag cgactaaagg
     3661 aactcaatct gatcgatagc atcgtgccag agccgattgg tggtgctcac cgaaattacg
     3721 aaggcatgat gcagagtatg aacaaggccc ttaagcatgc tctctctgag gtaggagatc
     3781 tggctgaaaa agatcttctc gatcgtcgtc ataaacggtt catgagttat ggccagttca
     3841 aagaagttaa agccaaagaa accaactaag ccaactaagc gcatcggtat tgcactgagt
     3901 ggtggtctag attcggttgt cttgctcgat gcggtctgta aggcctaccc gcacgacact
     3961 cttatcgcat tgcatattca tcatggttta caaaagcagg ccgatcagtg gttcgtcttt
     4021 tgcgagcggc tcgctaagca ttacaagatt ggttttgatt tccgcctcct gcatttgcaa
     4081 gatcaaaccg gcaatgtaga ggctcgggca cgctcagcgc gttacgaagc ccttcttgat
     4141 ctatgcgaag cgcatgatct ggatcatctc ttactcgcac atcatcaaaa tgaccaagca
     4201 gagaccgtgc tgctgcaatt actgcgaggt gccggccctg ctggattatc tgccatgggg
     4261 gcccagaagg aactctgcag tcctaccggc aagcgtatta ccttgtggcg accattactc
     4321 gagcaaactc gcgaagagct tgaagcgtat gccaggagac ggcgcttgca atgggtagag
     4381 gatcctagta atcaagacac catctatcga cgcaatgcgc tccgtaaaaa gatccttcct
     4441 gccttatgca agatccaacc aggtgccatc gccaatctag cacgcagtgc ccagttatta
     4501 gctcaaaccc aaacgctatt agatcgttta gctcaagcgg atgcaaaatc tctgatgcaa
     4561 aacggagcac tgcaaattgc tccgctaatg gcgctccaag caaatgattt ggctagtgca
     4621 aacaatgtct tgcgttactg gttaaagcaa catcacttag cgatgccgtc ccaagagcgc
     4681 ttgcaagcat ggtggcaaga cctctcagga atgcgtctcg atcaacgctt gcactgggcg
     4741 catgatggac aagcgattcg gggatggcgt gggcttttgc agattgatac ttcagttact
     4801 cagcaaaaaa caatacgagg cgagtggatc tttacaccaa tccccccaaa ctctaaacaa
     4861 gctggtctac cttgggactg gtatcaagag gcacttaacg caaagaccat tgagaccaaa
     4921 gcccgcgagg gtggggagcg cttgaagatc aagcccaata cgccaagtaa aagtctcaag
     4981 aagctttacc aagaagcagg ggtgcctccc tggcaacgnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttagccaaa
     5101 gccatctggt cggacacggc aagcgggtct ggccagagtg gtcctaaaag cccaatctgg
     5161 aacttaaaac cctgtaaaat catacgtttt gcatttcaga acttaagaaa accaagcagt
     5221 atttacagaa tctactatgt ctttaattgt tcataaatac ggtggcacct cgatgggctc
     5281 cgttgagcgt atccaaaatg tggccaagcg ggttgcaaag tggatgcgtg ctggtcatca
     5341 agtggtggtt gtgccatcgg caatgtcggg tgagaccaat cgtttgcttg gcttagccaa
     5401 agaactcagc cccaacccca atccccgcga gcttgatcaa attgcctcta ctggtgagca
     5461 agttagctct ggtctcttag cgattgcatt gcaagagcag ggtatcgatg cagtgagcta
     5521 tgcaggatgg caggtcaccg tgcatacgga ttctgcattt acaaaagcgc gcatcatggg
     5581 tattgatgat gcaaagatca tgaacgatct aaacgcagga cgtgcagtgg tgattactgg
     5641 ttttcagggt gttgacccaa atggtaacgt caccacacta ggccgtggtg gctcagatac
     5701 atcggctgtt gctgttgctg cagctctcaa ggctgatgag tgcctcattt ataccgatgt
     5761 cgatggggtg tacaccacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aggtcttgca
     5881 aatacgatcg gtcgagtttg ctggcaagta caaagtgaag acccgtgttt tgtcatccct
     5941 aaccgatccc ctaatccccc ttgatcaaga aatgaagtcg ggtactttaa ttacctttga
     6001 agaggacagc actatggaag ccgcagttat ttcgggtatt gcattcgctc gcgatgaggc
     6061 caagattacc gtgattgatg taccagatcg tcctggtatc gcatatcaga tcttgggacc
     6121 cattgcagat gccaatattg atgtggatat gattattcag aaccaatcgg ttgatgccaa
     6181 gaccgatttc acattcacgg tgcctagggc cgattatcaa aaggcgctag acctcttaaa
     6241 gaagagtgtg caggctcata ttggtgccaa agatatcttg ggcgatgcca aggtatccaa
     6301 agtatcggtt gtgggagtcg gtatgcgctc gcatgtgggt gtagccagta agatgttcag
     6361 aaccttatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nngcggtcag agccctgcat aaagcctttg aactggatca
     6481 gaagtaaaat agcggcagta atgcagtacg cagcaatgtg aagtaatacg gagacgtggc
     6541 cgagctggtc gaaggcactc ccctgctaag ggagcatctg tgctaaaaca cggatcggag
     6601 gttcgaatcc tctcgtctcc gccaatttac cttcatcaat attcctccac ggagtcgcta
     6661 gatgagcgct gcccataagt ggttaatctc ttttgtacta atccttgctc ttctattctt
     6721 tgcggtagac acatggatat gtaatcagtg ggtctaccat gactttgcag ctggcattga
     6781 tcgtcgttta tcttgttttt ggaagtctcc gtagtcaagt ctgttgttat gtctgcaaac
     6841 acattcaaag gcaacaaagc ctatctaccc agcaagacct gtgatgtatg cggtcgggag
     6901 atgacctggc gtaagtcttg ggccaagaac tgggagagcg tgaagtattg ttctgacgcc
     6961 tgccgatcca aaaagggttc aagtacaaag ccgcaataag ctgttatatc ggtttaaacc
     7021 ctaattattc ggtttagggg tctctaaacg atgtaaaaag aaagacgtga caagtgtttt
     7081 caaaataagg agaagaacat gaaacatgtc gtaaccattc ttggaatatc aattgttggg
     7141 ctattaacag cctgtcaccc atcgggtggc ggaactgggc aaaaggcgag tgctaattta
     7201 gagtcccgtt ctggatcaaa tacaaaaggc gaggttaatt ttgt
//
LOCUS       DDPQ01000030           17816 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_16987,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000030 DDPQ01000000
VERSION     DDPQ01000030.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 17816)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17816)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17816
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_16987"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    628..771
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15810..15939
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4404 a         4216 c         4191 g         4731 t
ORIGIN      
        1 tgccaacgcc cattaaataa tggggtagat gggccgggag gcgtggacca gtgtgagcca
       61 gtattcgctc aaactctggc ttgggctcgc ctacggagag tccgccaatg gcaatgccat
      121 caaatccttg ttcggcaaca acggctagag aatgctcacg caaagattca tacataccgc
      181 cttgcacaat tccaaataga gcattaccgg tatttagttc ccgaaaacgt tttagggagc
      241 gctcacccca acgcaaagac aactcaagcg attggcgagc agtttcttct gaggtgggtt
      301 tgccgtggga ttcgtaaggc gtgcattcat caaattgcat ggcaatatcg ctattgaggg
      361 ttgcttggat ttccatcgat acctcaggcg acataaataa cttatcgcca ttgatcggcg
      421 atgagaaggt cacaccttct tcactaattt tacgcagggc gcctaaacta aagacttgaa
      481 agccgccaga gtcggtcaga atgggctttt gccaagccat gaagcgatgc aaaccaccat
      541 gcttacgaat cacatctaat cctgggcgta accaaagatg aaaggtgttt cctaaaataa
      601 tttgggcctg catgtctact aggtcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nactattgtc
      781 tcaggttcgc cgtagataca tcgcatcacc atagctaaag aatcgatatc gcttttgaat
      841 agcgtgttga taagcctggt gaattgcctc tactcctgca aaggcgctaa ccagcatcag
      901 cagggttgat tttggtaaat gaaagttggt gatgagcgca tcaacgatct ggaattgaaa
      961 gccgggcgta ataaagagat tggtctcgcc ctctaatcgt tgagtgatgg catagctttc
     1021 tatggtacga atactagttg tgcccaccgc aatgaccctg ccgccccctt tttttgtttc
     1081 ttgaatcgct tgtagcgttt catctggaat ggaataccac tcataatgca tttggtgcaa
     1141 agagaggtct tcgtgtcgca ctggtgtaaa ggtgcctgcg ccaacatgca aggtaattgc
     1201 agcctgccga acaccgcgtt ctctaaggct tgtcaaaata ctttcatcaa aatgaagccc
     1261 cgctgttgga gcggcaactg ctcccggatt tttagccatg accgtttgat aacgtttggc
     1321 gtcatcgcta tccggagtgt ggtcaatata aggtgggagg ggtaactcac catatgcctc
     1381 gagtactgac atcgcatttt ctgggaaagt gatttcataa aaatcattac tccccccaag
     1441 attagctcga cctaaaacag tgattgtgat tggcgtattg tctgagccac tctcatgcga
     1501 ccgagagcgc agcataattt gaccgcccgg ctttggaacc tttgaagccc gaatctgggc
     1561 gagggcctga ttagggcctg taatgcgttc aattaaaagt tcaacctgac ccccagtttc
     1621 tttttgtcca aacaggcgag ctgggattac tcgggtatcg ttaaagacta gaagatcgtt
     1681 ctcattaatt aattgcacaa tatcgctaaa tgaacgatca aggcatagag gttttccgtc
     1741 cgcagtatgc gcaagctcca agagccggct ggcagagcgt tccgctaatg ggtgctgagc
     1801 aattagctca gggggaaggt cgtaattgaa gtcagatagt tgcatggtat taccatcggt
     1861 gttgggattc taaccatgag cacaaagccc ttgccaaaat taagcaaaac accgactcgg
     1921 atagggtctc aagccctcct tgaaaagatt gggttgggga ctcctgccgc tttggctttg
     1981 catctcccca tgcgctatga ggatgaaacc cagctttgga ctattgcaga agccatggct
     2041 cagtcgggtg agggtgcggt acaaactcaa ggggtggtga ttcgtagtca agttctgtat
     2101 cggccccgtc gacagttggt ggtcaccatt gaggatgatc attccgagct aaatcttcgt
     2161 tggcttaatt tttatccgag tcagcaaaag caaatggcag tcggagctca cttacgcgtt
     2221 cgtggagaga ttcgggatgg ttatttgggc gcggagatgg tgcatcctac tgtacgagca
     2281 gtgccggcaa atgcgccctt gccaaagagc ttaagcccag tttattcagt ctctgcaggc
     2341 attagtcaag cagctattcg taaagcagtt ttaggagccc tagatcaccc tagtatgcaa
     2401 atggttctag ctgaaattct tcccccttca atattagtta gtgccgagaa ggcggcgatg
     2461 caatttagtc ttcgggatgc agtgatgtat ttacatcaac cgcctgcaga tgctgatacg
     2521 aatgcattaa tggagcgaac gcatcccgct tggcgcagag tacagctcga agagttgctc
     2581 gctcaacaaa tatcacttaa gcaagctcat gaaatacgca agggccgcgc atctcctcct
     2641 tcgatattga ccgatcagcc agcaagatcc ttgtctccag taaaaacaaa atcactcacg
     2701 caccaattac acgaggcact tccttttgaa ttgaccgctg ctcagaacaa ggtatgggca
     2761 caaattagta atgatttgca ggcaagttat cccatgaatc gtttattgca gggtgatgta
     2821 ggaagtggca aaacgatcgt agctgctttg gcaagcgcac aaatggtaga gcgctcttat
     2881 caggttgcca ttatggcgcc caccgaaatt ttggccgagc aacattattt gaagtttaag
     2941 gaatggtttg aacccttggg cgtttcagtg atttggttag cggggggcat gaaggctaag
     3001 gaaaagaaac aagcccaaga tttaattgaa tctggtcaag cgcaaattgt ggtggggaca
     3061 catgccttaa ttcaggatgc ggtacatttt gctgctttgg gtctggcggt cattgatgaa
     3121 caacatcgct ttggtgtacg tcaacgtcta gaaatctctc agcggattgg ctcagtaacc
     3181 tactactgcc atcaactcat gatgtcggct acgcccatac cgcgtacttt ggcgatgacc
     3241 tattacgccg atctcgatgt atccgtgatt gatgagcttc ctccagggcg acagccaatc
     3301 acaaccaagc tggtgaaaga tgggagacgt caagaagtta ttgccgggct tgagcagtgg
     3361 ctccaaaaag gcttgcaagc ctattgggtc tgtcccttga ttgaggagtc tgaggccttg
     3421 caattgcaaa cggcggttga tagtcatgca gagttatcca gtgtccttgg caactaccca
     3481 gttggattga ttcatggacg cttaaaggca gaggagaagg cagccatcat ggcaaacttc
     3541 aagtctggca agaccaaggt cttggtttcc accacggtga ttgaagttgg ggttgatgta
     3601 cccaatgctg ctttgatggt tattgagcat gcggagcgtt ttggctatgc ccagattcat
     3661 cagttgcggg gacgagtagg gaggggatct gccgagtcaa cctgcatatt gatgtattca
     3721 gagcccttat ccttggccgc caaagagcgc ttacagaccc tgcgagaggt ttctgatgga
     3781 tttgtaatcg cagagcggga tttggctttg cgagggcctg gtgaactcct gggcgcgaag
     3841 caatccggtg aggcgatgct acgttttgtt gacctgcagc gggatgcatg gttaattaag
     3901 attgcccaag atctggctga tcgtttgtta atacagcacc ctgatctagt gcaggcccat
     3961 ttaggccgtt gggtcggatc acgcacagaa tttctaaagg cttgataatt gcactatgac
     4021 cttaaccgaa ctccgttata tcgttgccgt tgccagggag cgccattttg gacgggctgc
     4081 cgaggcctgt catgtttctc aaccaacctt gtcggttgct attaagaagc ttgaagaaga
     4141 gctgcagacc caaatttttg agcgtactag ttctgatgtt gctcttacca ctctcggcgc
     4201 ggtcattatt cagcaagccc aacgcgtatt agaagaagct aacgctctaa agcacttggc
     4261 caaacatggg caagacccct tgtccggacc attacgctta ggggctattt ataccgttgc
     4321 tccttattta cttccaagct tagttcgggt tgcacgcgat accttaccaa aggcccctct
     4381 atttttggaa gagaacttta ccgtacgctt aattgagatg ctgcgccaag gtgagttgga
     4441 ttgcgccatc ttagccgacc catttccgag cgctggatta gattttctgg atctgtatga
     4501 ggaacccttt ttagtagcca tccccaaggg acatgagtgg tcagatcgtc ccaccattgg
     4561 ccaaaaagag ctcaaagagc aaaacacctt attgctgggc gcaggccatt gttttagaga
     4621 tcacgtcttg ggggtttgcc cagagttaaa ccgttttggt tccggcctta cattaggtga
     4681 gcatcgcagc tttgagggct cttccttaga gactattcgc caaatggtag caggtggtat
     4741 tggcataacc gtattgcctc gtacctcggt gatcaatccc aatgatcgag acggcttaat
     4801 tatttatatt ccgtttaatg accctgagcc cactcgacgc gtgagcttag tgtggcgcaa
     4861 gggctacccc cgaatagagg cgatgaaagc tttagcacgc acgattcggg cttgcgatct
     4921 tcctggagcg aggctgctct gattcccgag cgtttgcagt cctacatcgc gctggtgcgc
     4981 ttaaataagc ccatcggtat tttgttactg ctatggccaa ctctctgggg cttattcctg
     5041 gctagcgatg ggtggcccac tctttcaatg ctacttatct tttgtgtggg taccgtacta
     5101 atgcgaagtg ctggatgcgc aatcaatgat tatgcagatc gagattttga taagcatgta
     5161 ttacgaacca aagaacgtcc tctaaccagt ggcaagatca gcaaaaaaga ggctgtattg
     5221 attgcgatag gccttgcact ggtttctttt ctgttgatac aacctttaaa tatactcaca
     5281 aaacagttat ccgtttttgc tgtgatcatt gctggcatct acccatttac aaaacgattt
     5341 ttttcgatac cgcaagcagt attaggagtt gcctttggtt ttgggatacc catggcatat
     5401 gcggctattc aaaatatgat ccccatcgaa gcttggattt tgttgattgg taatatcttt
     5461 tgggccattg cctacgatac ggcttacgca atggttgatc gtgatgacga tattcgcctc
     5521 ggattaaaaa cctccgccat tacatttggg cgatttgatg tcactgcaat cacaatttgt
     5581 tacggcatat tgtttgcttc tcaaatttgg gtcgctgagt tagttgggct tagcaaatgg
     5641 ttctatcttg gctggatcgc tgctctgggg tgcgcaatct atgagctgcg tttgatttca
     5701 agccgtaagc gtgatgcctg cttcaaggct ttcttgcaca ataattggct gggagccagt
     5761 ttatttttag gaatcttttt gggcttgcta ttggagtagg caattgatta agagccaccc
     5821 agctctttag ccatttgtac tccacgctct ttggctgcag caagggcttc ttccaaaata
     5881 ccttgccact gtttttcatg taaaacatct agggctgctg ccgtggtacc ccccttagag
     5941 gtcactttct gacgcaaagt agacggagaa tcactcgact gcatcgccag ggcggctgcg
     6001 ccaactacgg tttggtaggc taatagccga gccgtgtcag taggtagccc catacgttca
     6061 ccaccagact gcagagcctc taaaaatgca aacacatacg caggcccgct tcccgaaact
     6121 gcagtaaccg catccatcaa agactcatca tgaatccata ctgttttgcc aacggatgaa
     6181 caaatagttt cagcatgctt gcgttgtgcc tcactcacag ttgctggcgc aaataagcca
     6241 gtcataccct gattaatcag ggcaggtgta ttgggcattg cacgaacgca gcactcatag
     6301 tgcaaccagt gggccatact ggcagtcgga atacctgctg caatacttag aataagagga
     6361 cgctcacctg aacaattttt gataaggtgg gctaactcaa aagcgataga cttgaagtct
     6421 tggggcttta ccgcaacgat cacaacgtgt gctgcattta attgctcttg cgcattcatt
     6481 agagtcgata aaacatgaat ggagaagtca tgggtaagat gtgctgcagt ttgagggtcg
     6541 gtctcaacag tggatataga tttaggatca aatccttttt ttagaaggcc gccaataagg
     6601 gctcgaccca tattgccgcc cccaataaaa agggtattca tgtgattaga tcttgtcatg
     6661 cctctatctt agcgctaggc ggacgttgtc caaaaatagc gctccccacc cgtatcatcg
     6721 tagcgccctc agcaatagcc gcctccagat cttccgacat gcccatagat agggtatcga
     6781 agttatgaaa accagcagtc atttcttttt gcatacgaat agactcaaaa agttcccgaa
     6841 ctgctgcaaa cgatttacgt tgttcattgg gattctgggt gggctctggt attgccatta
     6901 gtccacgcaa ggtaagaccg cgtaatggcg caatttgctc acacagggca agcgcttcag
     6961 agggatgaac gccggattta ctttcttccc cgctaatatt tacttgcaca cagacctgaa
     7021 gggggccaag acgagcatgc ggctcacgct gaacagaaag acgttcagca atcttcaggc
     7081 ggtcaatggt gtgcatccaa tcaaagtgct ctgcaatctc agccgtttta ttgctttgaa
     7141 gcgggccaat gaaatgccat tcaagccagg ggcgtaattt atcaagctgc ttaattttat
     7201 caacagcctc ttgaacgtag ttctcaccaa acgctctttg tcctgcatgc atcgcttctt
     7261 cgatggctgc actcgggaag gttttgctaa cagcaattag ttgtatggtc tctgggtcac
     7321 gaccatattc ctcggcagca gcatcaatac gatactgaat tgccatcaat tggttagtaa
     7381 tgcgccccat tatgttttag ctagaagttg atcgatgatc tcaacccaat gacgaaccgg
     7441 taggtggtct tgatgaagat ggctgatgca gcccacatta ccggatacta ctgtcttagc
     7501 accagagctc gcgcaggcag tttctagatt ctgaatcttt tggatacgta attgctcaga
     7561 gagttttggt tgtaataccg agtaggtgcc tgctgagcca caacataaat gactatcggc
     7621 acatacattc acaccgatac ccagactttc tagaagacct tcaaccttac ccctaatttg
     7681 ttgaccatgc tgtaaggtgc atggtgggtg atagacaacc cccgctttct tatcttgccc
     7741 aattttctga atcaactttt cctgaaattg cggcatgatt tctgatagat ctttacaaag
     7801 ctcagaaact ctctttgctt tgcttgcgta gagcggatca tcgtgtaata aatgcccata
     7861 gtctttcacc atcacgccgc agccggaggc attcatcaca atcgcctcta ctgagtcttt
     7921 gccattctca atagatggcc accaagcgtc aatattgcgt ttggcatcgg taagcccgcc
     7981 atcttggtcg ttgaggtgat agcgcagtgc accacagcat cctactttac tactgctgat
     8041 tagctcaata cccaatgcat ctaagacccg aacggtggcg tgattgatgt tgggtagcat
     8101 gcccggttga acacagccct caaggatcaa catcttgcgt gcatggtggc gctgtgggcg
     8161 ctgtcctgca ggtaagccct gcggtatttt tctctgaagc gcagcaggca taagtggtcg
     8221 tactacgcga cccacggcca ttgccgtgtt aaagagcgtt ggacttgtta aaccctcttt
     8281 taacagccag cgggttattc gctctgaaga tgggcgcggt gcggttttct cttcagccca
     8341 tttgcgtccg atatccacga gcttgccata ctcgacgccg ctagggcacg tgctttcaca
     8401 attgcggcaa gtaaggcagc gatctaggtg tccgcgggtc ttctcggtcg gcaactgacc
     8461 ctcagcaatt tgtttaatga ggtaaatgcg accacgtgga ccatccaact catcacctaa
     8521 taactgatag gttggacatg ttgcagtaca aaagccacaa tgtacgcact tccctaagat
     8581 acgctttgct tcaataccat ctgcggtatt gataaattgt ggtgctaatt tagtttccat
     8641 aattaaggta ggcgacctgt cgtaaagact ccaaatggat caaaggagtg gcgcaagcgt
     8701 ttttgcacaa gctctagacc agcagtaaga gggtttgatt gcaaagaggt gaaacgttca
     8761 aactgttcag aaccattatt tctaaaacaa ctggcatgtc caccatgttc ttgagcaagc
     8821 gctatcagag tgttcgaaat gctttgatta gctggcccct gaaaccaacg ttgttgacca
     8881 tgccactcca taatgctagt cggattaaaa ccgattggca ttgggatgga aggacagttt
     8941 gctggtaaag ataatcgcca gagcgcgttg cccggagcaa gctccttgaa ccaaacatgg
     9001 gcttgctcac gcaattcatt ccagaaagta actgcatccg taaagtccat caatgtggcg
     9061 cttagttccg ccaccatcat tttgcttgcg gtagcaactg cagcattagc acctgctaag
     9121 cgaaccgtca gctgaccctc ttcattggtc ttgccaatcc atgcacttgc attaaggggg
     9181 agtggttgtc cagcccattc attcagaata cgtaatgcgc gatcttgtgt gatggtgcaa
     9241 cgtaaactcg ctgtcgcagc gggctttggt agtaccttga cagacgcctc aagtagcaga
     9301 gcaagagtac ctagcgatcc tggcaagagg cgtgagacat catagccagc aacgtttttc
     9361 atgaccttgc caccaaagga aagatcttca ccgcgaccgt ccatgaggcg agtgcccaag
     9421 acaaaatctc ttaagttgcc agcactgatg cgagcgggac cagcaagtcc tgccgcgatt
     9481 actccgccaa aagtagctgc ctgtccaaag tggggtggtt caaagggaag catctgattt
     9541 ttttcagaga gcgccgcttc aatttcagca attggagtgc ctgcacatgc cgtgatgact
     9601 aactcctccg gttggtaatc caagattcct tgataaggtt tagtgcttaa ggtttgtggg
     9661 agttttggat tgccaaacca atccttgcta gcgccacctt cgatcgataa ggctttgtta
     9721 tgtttacctg cctcgataat ctgttcccga aactgcgaaa gaattgcttc actcatactt
     9781 agaagcgctc caactctggg tgaggcagag cgccaccact aatccgcatt ctgccgtatt
     9841 cggcacagcg gttgagtgtt gggattgctt tgtcggggtt tagtaagcga tctggatcaa
     9901 atgctgcttt aacgccccaa aagagttcac gctcttgctc accaaattgc acgcacatcg
     9961 aattaatctt ctcaatacca accccatgct ctccggtaat cgtgccacct aactcaacac
    10021 aggcctctag aatctcggta ccaaattctt cagcacgatg ccattcatca ggatcggcgc
    10081 cattaaacag aattagggga tgcatatttc catcgcctgc atgaaataca tttaaacagc
    10141 ctaagcgata tttttcttcc atacccttaa tgcgacgcaa taaggtcgca atgtgtcgtc
    10201 tagggatcgt gccatccatg cagtaataat ctgccgcaat tcgaccggca gctgggaagg
    10261 catttttacg accactccag aagcgtaatc tctccgcctc attttgtgag acctggatac
    10321 ggcttgctcc ttgctgttca agcacagcat tcatgcgcgc aatctcctcc tcaacctctt
    10381 cgggggtgcc gtctgattcg cataacaata tagcttcagc atcgaggtca taacccgcat
    10441 gcacaaattc ttctaccgca cgagtggtag cccgatccat catttcaaga cctgctggga
    10501 tgatgccggc cgcaataatg gctgcgaccg cgttaccacc cttttctatg tcgttaaaac
    10561 tcgccataat gacccgagct aatttgggct tagggacgag ctttactgta atctcggtaa
    10621 caatacccag cattccctcg ctaccaatta gtactgcaag caggtcgagt ccaggcgcat
    10681 caggggccat accaccaaat tcaacgacgt cgccattcat taatacagcg cgtactctta
    10741 atacattgtg aagtgtgagc ccatacttta gacaatgaac accgcccgag ttctcattaa
    10801 cgttgccgcc aatcgagcaa gcaatttggg aggatgggtc tggcgcgtag tacaagccat
    10861 gcttggccac cgcttcagag attgctagat tgcgcacacc cggttgcaca acagcggttc
    10921 gtgtgaacgg atcaatcttc gtaatctttt taaacttggc caaggaaaga acgagacctt
    10981 gctcgatcgg catcgcgcct cccgataaac ctgtcccaga tcctcttgga acaatgggaa
    11041 tttgcatggc atggcaagcc ttaagaatac gcacaacctg atcctcagtc tctgggagag
    11101 caactgccag aggcattttt cggtaggctg ctaaaccatc gcactcatag ggaatagtgt
    11161 cctctggctg ccataggaga gcatcttctg ccaggatagg actaagggcc gagactaaaa
    11221 gcgcctgttt ttcttggata gtaaggattt ctgcggcggt gtggggggcg ttcatcggtc
    11281 tattttagcc acttacctat aatcagtgca tgggaaatag actttcaaaa atagcgacgc
    11341 gaaccggcga tgccgggatg accggtttag gtgatggtag ccgggttgaa aaggatcatt
    11401 tgcggatctg cgccatgggc gatgtggatg agctcaactc tcagattggg gtcttgatga
    11461 ccgaagagtt tcccgactca attgcgaacg atctacgaaa tcttctgctg cgggttcagc
    11521 atgatttatt tgatttgggc ggcgaacttt gtatccctaa ctacacttta ctcaagactg
    11581 aacaggtaga gcaattagat gtctggctga atcagtacaa cgccagcctg cctcccctga
    11641 aagaatttat tttgccaggc ggtactcacg ctgccgctca agcacacatt tgtcgaacag
    11701 tgtgccgtcg tgctgaacgc tcgattgtga aactgggttg ggctgatccc attcatgatt
    11761 ctcctcgtca gtatgtcaac cgactatcgg accttctctt tgtgctggct cgcgtactca
    11821 atcgtgttgc tggtggccat gatgttttgt ggaaaaacca aaacaaggtc gacgagactt
    11881 agtagaaata aaaacgcgga ccagggtccg cgcatcactt gagttcttac gcgcgtcggc
    11941 gtttacgaac agcctccgcc aatcgttcaa gaacttgtac agagtcatcc caattaatac
    12001 aagcatccgt aatactcttg ccgtactcta gagccttggg atcatctttg ccgggtgtaa
    12061 atttctgcgc acctgaatgg agatgacttt caatcatgac cccaaaaata tgttttgatc
    12121 cactctcaat ttgctcggca atatcattgg ccacatcaat ttgtcgttga tgttgcttgc
    12181 ttgagttagc atgcgataga tcaatcatca atcgcgctgg aagtttggcg gcttctagtt
    12241 cagcacaagc agcttgcaca tacttcgctt catagtttgg ttctttgccg ccccgcaaaa
    12301 ttacgtggca gtctttattg cccttagttt ctacaatggc aacttgacca tttttgtgca
    12361 ctgaaaggaa gtggtgtggt cgatgcgccg cctgaatggc gtctgtcgct atcttaatat
    12421 tgccatccgt accattttta aatccaatcg gcgcagacag gccagagctt agctctcggt
    12481 gaacctggct ttcggtggtg cgcgcaccaa ttgcacccca tgagattaga tcagcgatgt
    12541 attgggggga aattacatcg aggaactcac tacccgctgg catgccgaga cgattaattt
    12601 ccattagcac ctggcgtgca atacgcaaac cttcttcgat cttaaaactt tcatcaaggt
    12661 aggggtcgtt gattaaaccc ttccaaccta ccgtagtacg tggcttttca aaatagacac
    12721 gcatcacgat ttcaagatcc gcgctcaggc ggctacgttc ttttaggaga cgctgacaat
    12781 attcaactgc agctgctgga tcatgaatag agcaggggcc gatgatgacc aataaacgat
    12841 catctttgcc atggataaga ttgcggattt tctcgcgggt acgaccgatt aacttctccg
    12901 ttggcgtgcc agaaatagga aaaaaccgaa ttaaatgctc tggcggaggc agaaccgtaa
    12961 tattacctac gcgttggtca tcggtcgcag aggtcttatc gatactcgcg taccagcttt
    13021 cttgagggtt ttggggttta gtgctcatgg cttctattct aaagcctgat tacaaagact
    13081 gcctttttag gcggttccac ctacagtcat ttgatcaatt cgtaaggtgg gctgaccaac
    13141 tccaacaggc acactctgac cttctttacc gcatacgcca accccactat ctaaacgtaa
    13201 gtcgttgcca atcatagtga cctgcttgag agattcaggg ccattgccga tgatggttgc
    13261 acctttcacc ggatattgaa tcttaccgtt ttctacccaa tatgcgactg aggcagagaa
    13321 cacaaattta ccgcttgtaa tatcaacctg accgccgcca aagttcactg cgtataaacc
    13381 acgatcaatg ctcgccacaa tttcttgggg gtcgtattgg ccagccagca tatatgtatt
    13441 cgtcattcgg ggtaggggga gggaggcgaa gctttcgcgt cggccattcc cagtcaaggg
    13501 catcttcatc agacgcgcat ttaaactatc ctgaatgtag cctttgagca tcccatcttc
    13561 gatcagtgtg gtgcattgtg tgggcgtacc ttcgtcatcc atattgaggg agccacgtcg
    13621 accgcttaag gtaccgtcat caaccactgt tacacccttg gcggctacgc gctcaccgat
    13681 tcggcccgag aaggctgagg agcccttacg attgaagtcg ccctcaagtc cgtgcccaat
    13741 tgcctcatgc aagagtacac ccggccagcc tggacccatt accacagtca ttggtcctgc
    13801 aggagcaggc cgtgaaccca aattgagtag agcgttgtca accgcttcat caacccattg
    13861 atgaattcgt tctgcattaa agaagccgta gtcagttcgt gcgccaccgc cagcagagcc
    13921 tgattcacga cgaccatttt gttcggcaat gacgtgaacg gatacgcgta ctaatggacg
    13981 gatatcggct gccattaaac catttgcgcg tgctaccaga acaacatcaa attcacccgc
    14041 aaggctcgcc attacttgaa tgatgcgggg atcacgtgct ttagcacgac gttcaatttg
    14101 ctcgaggagc gcaatttttt ctggaggctt taaggagtcc aatggattta ggtgggagta
    14161 cagtgacgta cttaaatggc tttgtcctat gatcgggttg cgaagtttta ccttcccacc
    14221 ttttggtcca atcactcgag tggcattggc tgcttttaat aaagcttctg cgctaatgac
    14281 atccgagtag gcaaaggctg ttttatctcc tgagatagcg cggaccccaa ccccttgatc
    14341 aatattgaag ctaccggatt taacaatgcc ttcctctaga ctccattgtt cgcttcgtgt
    14401 gtgttgaaaa taaagatcgg catcgtctag acgatgtgca tgcatcacac caaacagatg
    14461 atcaaggtct gaggtgctta gaccgtttgg ctttagcaaa acagagtttg cgagatcaaa
    14521 ggcttggttg gcctcaaggg gtgctgcaag atgatgtagt ggaagatcag aggtattcat
    14581 tacgatagct tacgatgttt tagagcaggc agcttggagc gaaccccctc aagggtttct
    14641 ttttggactt gaccaattac aaatccctcg ccgctaggaa gttcttgaat gatgtgaccc
    14701 cagggatcaa ctagcataga ctgcccccag gtctgtcttt ggttctgatg caagccaccc
    14761 tgagccgacg ctaagaaata acattggttt tcaattgcgc gggcgcgtaa caaaatctcc
    14821 caatggtctt taccggttgt gtgggtaaat gccgcgggta ttacttggca gtctacagtt
    14881 tcgtgttggc ggtagagctc cggaaaacgc agatcgtagc aaatgcttaa gccaaaaacc
    14941 cagtcttcac cttgatcatg gatgcgcact gtgctgattt gattgccggc ctcaatggtc
    15001 tcagattctt ggtaacgctc tttttcggtt tggaagccaa acaaatgcat tttgtcgtaa
    15061 cgagcggtac aaataccctg aggattaaac acgagggtgg tatttagaac cttatgctga
    15121 tcacttgctt caaggggtat cgtgcccgcg aataaataaa tctgatgctg atgcgcaagc
    15181 tcttgcatcg ccgattgaat tggcccgcta ccaaacgcct cccgaacttt caccttatcg
    15241 gtgtcgttaa gacccattag gcaaaaatac tcgggtagcg tgatgacctt agcccccgct
    15301 ttagccgcct cgccaacaag acgtcgggcc gtctctaggt tcccctctaa agaggcagaa
    15361 gagatcattt ggatggcggc aacgctaaga gatcgaggca tatagctcaa tactaatact
    15421 gaatgacaaa tttctcagtt ctgaggagaa acgcttggag cgcggctggg ggtattcggt
    15481 atagaaggtg cgcttggcat tttttgctca cgcaaaagat ttcgggaacg aatggtctcg
    15541 atgatttttg gatccaaggg ttgtccattt tgatccagtg gaatgacatc tggcttatcc
    15601 cagctgccct gaacaagata atccgattgc aggttgcgat ttagctggtt gctaatcagg
    15661 tattgactaa ttaaagcact tgctccaatg atggggttga cagcaaaaag tgccagagca
    15721 ccggcggtcg catcaatcgt tggaaatatc gtaatacgta aatcttgtat ttgtttgggg
    15781 atgtttacta agccgcttgt cgcaacacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctgcggtcac aacatttccg
    15961 agacttccct gaatatcaaa gcttgcaaat tttaggagac tttgcaggct aaatacaccg
    16021 atgactttgg caatcccaga atcgacttgg agcaaccgcc cgttttcaag tttaatagca
    16081 atctttccac taagggtttc ataggcagga tcatacggag gaccactcca atccagttgc
    16141 gcagtaacaa ttcctttgcc accttccacc gcttttgggc taccccagtg tgcaatcact
    16201 ccaccaagat ctttaatctc tgaactcacg tctagcaaaa tacgttcatt attaccctgg
    16261 gtatccacag accactctcc cgacaccttc gagcttgcct ctgcattggt gacgaccaag
    16321 ttttgaatag tgattttatt gggactattt tgggtcttga tcacaatgcc accgggctta
    16381 tattggttga ttgataactg gtcaatcgta agatctagtt caggaataga gttgagggga
    16441 acagttttag cgacagcttt agccttagga tccccagcca cactaattgc cggaggggtt
    16501 gactctggaa catttaagcg aactaattta cctttaacga gatcaaatcc tgggcgctct
    16561 acatactgaa cttgcccctc aaggaggggg gatttagctt ggatcagcca gctaccattt
    16621 actagttttg cattgagggt gagattgggc cattgccgat tcagagcgac agcatttcga
    16681 acgcgcgcat tcaatacatt gaggccatcc ccatcaggac tcgagctacc tttcacaatc
    16741 tcttgagtag atttttgcac aggagcttta ttactcaggg cctttgggta aagcagacta
    16801 tgccagtcat cgatattgat atcattagct tgcaagttaa ttccaattcc tgagtcagga
    16861 acaagaggtg tggcacctat accaataccc tgactcatca caccttgtgc attgattcgg
    16921 ccttcaagac taatattttt tcctaactga ctagaccatt gcgaggtccg ctttgatgta
    16981 ctctcattca gtacagattg atatttaaac tgccccttaa gattgctgcc cgttttttta
    17041 tctagcggct gaggcaaatt agaccccagt gcattgagat caagatccag gtttaactca
    17101 gttagcttac tacttaaaac taaatttcct ttaaaaccaa tattgccgtt tagagcgttt
    17161 agtaattgtc gtgattgggc gcccacctta tccgagaagt gctctttgag ccgcgcaaga
    17221 tcaaccttgc cacttaggtc gtacaaatcc tttttatttt gagatacagc attttgtttt
    17281 aagctaacgg tgccaccaaa aaactcagcg ctgatctgct caaaccgagg cagatcttct
    17341 gttaagcgaa cagttccctg tactttatta aatggcgcta gatcagacca ttgaactgta
    17401 ttgttattta gataaagcgc agcatttaat ttgaggtcat tagtattttg gagtggtaaa
    17461 gtaaatccca aatctaattt accgagaccc gatattttca actgatttgc aagagccggc
    17521 ctttgtaaaa gaacgggagt tgttctgaga tagtccatca tgtcatcaat gggaccatta
    17581 actacagcct taaactgaac actagcttgc ttgcttgcga tgtttgcaat ttcagcattg
    17641 agattttgta tttgcaatcc cttgtagcga gcactctgaa tgttcgcagc aagtttagcc
    17701 tgctgcatgg tgatttgacc ttcgagatca gtaaattctg gccaagcccc tttatttgca
    17761 ggaaataggg gtgcagggcg ataaactact ttggtaattg gtagcgttag agttag
//
LOCUS       DDPQ01000031            3724 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_23315,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000031 DDPQ01000000
VERSION     DDPQ01000031.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_23315"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    330..404
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1053..1089
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1603..1660
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          915 a          825 c          828 g          986 t
ORIGIN      
        1 actttgccgt catcgtgcaa acgaataatc gagccagcgt gatcatctaa ttgttgtgct
       61 cgcgccatct caccgcggtc acccaaggtg agatacacat agcctgcctc atcaaagacg
      121 atgcgcccac caaaatggtg attagagcga gtctttggct ccatactaaa gagcacctgc
      181 acgttggtca tttggtgacc gacgagtttg ccgcgtgcca atgcagttcc ccaaccccct
      241 gagccaggtg cactgtatgc aaaatagatc caaccatttt tagagtactg cgggtgtaag
      301 accacatcaa acaatccgcc ttggccgctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgttcg gtaatgagta
      421 gtcgcccatc gggtaagaaa gccactgacc acggatattg caaaccttgc gtgagtggca
      481 cgacccgaaa agactgtttc tccgattgga tggtttgcgc aaggaccgaa ctaagatgaa
      541 aaacgcccag caccatcatg agacacaggg tgagtattct catgcgcaga atggccttag
      601 gtagctaata cccgacgcag aaaatttccg atatccacaa tctcatgggg atgtaccgaa
      661 tgctccatgg cataggtctg ccactgcacg gcatagccat tggcttcgag tacgtcttta
      721 gagtactcac tgcgatctag cgtaatcaca ctatcccact caccatgcgc catgaagatg
      781 ggggtgtgtt gatttgcagg gttgcggttt ttaacaaagg agtctctaag gggcaagtag
      841 cccgataagg ccatgatgcc acccaaggct tgcgggtagc gcagccctgt gtgcaagacc
      901 atcgcacagc cttgtgaaaa tcctgccaag attattttgt tgggggcaat gccacgactg
      961 cattcgtgtt caatgagcgc ttggatggcc ttagccgaac gctcaatacc agactcatcc
     1021 tcgcgatcgt ttgggcttct gccaataatg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnng ggaagataaa gcggataccc ggacatgccg agagatcgag ttggggcaag
     1141 atggaggcaa agtcgttgcc atcagcgcca agcccatgaa gccaaattac ggctgcggtg
     1201 ggatgctcag cggtctcaat ttcaatacaa ggtagtcgtt ccatttggtg ggcgctcaaa
     1261 gtgatatgta aataatgctt agttgctaat gcgagtattc ttattcattc tcttttaaat
     1321 cctaaggaac aaaatgacta tttccatgta tcaggctagc gtacctcgac ttattaatgc
     1381 gctgactaat ttatccaatg tcttgcaaaa agcacaagac catattacag ataagaagtt
     1441 agatgccaac gcactactgc actaccgctt atatcctgat atgtttaatt ttacccgtca
     1501 gatccaaatt acggccgata ccgctaaagg ggtcgtagca cgtttagcag gcgttgatat
     1561 ccctgcctat gaagatactg agagtagcat tgctgagttg cannnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaggacaaag cgattgtgac
     1681 caagcgcggt gataaagaaa cccattacaa gggcatgcag tttttattag gacatgccat
     1741 ccccaatgtg tactttcata gctcgatggc ctatgccatc ttgcgcacca atggcgttgc
     1801 gattggtaag cgtgattact tgggcaatcc ttagtcacct ctgccctaga gtaaatgccc
     1861 ggtctttaca aagatggtgc agccctcttt gctaaatggc ttgtgcatgc tcatatgggg
     1921 gcttctaatc catgagccgg cttcgtagcg accatgttcg tcctcaaaga ccccatcaag
     1981 cacgaagatc tcttcacccc catggtggcg gtgagggttg aaataggtct gcggtgccca
     2041 gcgcactaag gtcgatcctg ttccttggcg catgagaggc atcaccgtca gccccggtac
     2101 gagtccttgc aaccattgcg ctgtgtgggt gttcacaatc tcgcgttcgg tttgctcaga
     2161 ccctaaatga cgaagtttga caaagagtgt gcagccctct ttgctaaatg gggcgtgtga
     2221 cgagcctggc gggttcatga ggtatgttcc tgctgggtag tcgccggtct catcactgaa
     2281 gacaccatca aggaccaata tttcttcgcc aagatcgtgg gtatgcgaag caaactttgc
     2341 gcctgctgca taacgcacaa tgctcgttgc tttcgctatc tcatctccta ttcggtcgag
     2401 cattttgcgc tctacaccag gttctgggct catctgccat gtcagcgcat gatgattaag
     2461 gacgatacgt tctttgtagt ccgcgtggag gttcatcttg atttagttac ttgaatcttg
     2521 agctaaagac tttgtatttt tgattgcttg cgccaactca tctcgcgata cttgaaaaat
     2581 atcaacaagc ttttcaaagg ttttatagag catttcgcgc tggcctgcct caattaaaga
     2641 atagctagat tgagagttgc ccaatagtaa tgccactttt gtttgcgaaa ggccagacct
     2701 caagcgcaag gatgcaatcg tcaatggcat cccaacatca ttacaagtct gagcaaatcg
     2761 ttttcttgct tttgcaagca tctcagctcg agatggatca tgctcaaatg aattaacgat
     2821 ttcaccaatc tctttaaatc cttgggagag aggtcttatt gcttggtcaa agctctgatc
     2881 agaattttgc gttgttgcgg caaggtttaa tgttggcatt ttagggttaa ggtaattaat
     2941 ggcggggtat gttgagggct tcataggtct ggaggattct tttaactctc gggtttttcg
     3001 tgtcatagtt aaagtttctt tttaagatgg ctaaaacgta atagcagtat tcaatggtgt
     3061 gaaaggcata aataattcga tagtcacgca cattgctaag ccaaactaat ttaagtctcc
     3121 aaaggttgta tctagcaacc ctggtttttt gccattcgtc cacatcaaag cttgggtcct
     3181 tgtaatggca atacccacgg ctcgttagcc tgcttagtaa atcttgattg ccgctgatct
     3241 cttcaaaaaa tactgtaatg tctgccgccg ctacctcatc aattaagaaa atttgctcta
     3301 ggtcgtcctg cgcatcttgg tgtattgcta atgagtagta tcttgattgg gagtatctca
     3361 tatcttctaa ggtataggtt aatattaaat actgtataaa aacacagtat aaatcttctc
     3421 tttagccctt ttaattagat ggttgtaagg tttatggggc cagtaactaa ccaacgaaat
     3481 tgaacatcca acaaatctgg tttcttgcaa ttctggtact tttatctata ctggctcact
     3541 ctatgagcta gtcaaagctc attctattga tcttcaatga ggagactgat atgggcgata
     3601 tggagcgctt gcatcgcatc aaatacatga ttcagagtcg gggttgcgtt cccattgatg
     3661 acttcttaca caccttagag atctccaaag ctacctttaa gcgagacctc gagtacttac
     3721 gcga
//
LOCUS       DDPQ01000032            3466 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_6478,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000032 DDPQ01000000
VERSION     DDPQ01000032.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_6478"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1514..1554
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1825..1897
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3240..3331
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          914 a          712 c          765 g          869 t
ORIGIN      
        1 tctttggttg cggatgaaac acccttatcg ctttacctaa agctcactca aaaatccggg
       61 caaaaaaata cctatttatt agaatccgtc tacggtggtg aacgctttgg tcgttactcg
      121 atcattggct taccagctcg cactctatta aggagtgtgg gtactccaga attaccaatc
      181 aatgaagtga tgctcgatga aaaggtaata gaaaccaatc aagataatcc gcttgacttt
      241 attgatagtt atttcaagcg ttttaaggtt gcgccccaag caaacttacc ccgtttttgt
      301 ggagggcttg cgggttactt tggctacgat accgttcgct atattgagaa acggcttgcc
      361 aagtattctc ttcccgatga aattggtgtt cctgacattc aattaatgct gactgaagaa
      421 ttggcggtac tcgataatgt cgaaggaaaa atttatttca ttgtttatgt tgacccaaac
      481 gatgaaaatg cccataaagc tggcacagct cgcttggctg aattgcaagc gcaactaaat
      541 aacattgtcc ccactgaaaa agaattacct gtaaaccaaa acaaagaagt cgacattgaa
      601 ttaattcgaa agttcaaggc ggccaacttt gagaatgcag tacgcaagac caaagaatat
      661 attttggctg gtgattgtat gcaggtagtg attggtcaac gtatcagccg tccctactcc
      721 caatcgcctc tgcaacttta ccgtgcccta cgctcattaa atccatcgcc gtacatgtat
      781 ttctatgact ttgggaatct acaaattgtg ggctcgtcac cagaaatctt ggtgcgtcaa
      841 gaaaagcgtg aagatcgaaa gattgtgacc attcgaccac tggctggaac tcgtcgtcgg
      901 ggtgccacac cagaagaaga tgcgcaatta gcgaaagagc tcttagcgga tccgaaagaa
      961 attgccgagc atgtcatgct aattgactta gctcgaaatg atgtaggccg tattgctaaa
     1021 accggtacgg tcaaggtgac tgactcgatg gtgattgaga aatattccca tgtacaacac
     1081 attgtcagct ctgttgaggg tgaactcaag gacaatatga gcaatatgga tgtcttacgc
     1141 gccaccttcc cggctggaac cctatcgggt gccccaaaaa tacgggcaat ggaaataatt
     1201 gatgaaatgg aaatcaccaa acgtggtatt tatggtggcg ctgctggata cctctcattc
     1261 tcaggtgata tggatgtcgc tattgtgatt cgtactgggg tcatcaaaga cgggatgctg
     1321 cactcgcaag caggtgctgg agtagtggca gactccgacc ccacatcgga gtggaaagaa
     1381 acggaagcga aggcacgcgc agttttagcg gcagctgatt tggcagaagc ctcaaggggc
     1441 tcataaccat gcttttaatg attgataact acgattcgtt tacctttaac ctagttcagt
     1501 actttgctga actnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaagcca
     1561 gaccgtattt gtatttctcc ggggccatgt agccctaccg aagcaggtat ctctgtagcc
     1621 acgatccaac aatatgctgg gaagattccg atcctgggtg tatgtctagg gcatcaagcg
     1681 attggagagg catttggtgg gaaagttatt cgagcgaaga aagtaatgca tggcaaaacc
     1741 gatgtgattc atcatacggg cgaaggcgtt tttaaggact tgcctgagcg ctttagggtt
     1801 acccgttatc actcgctcgc aattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg caatttcatc ctgagtctat
     1921 tttgtctgaa caagggcacg ccttactcaa aaacttttta cacatgaaat aacttgatct
     1981 ctatggcaat gaccgcatca gcagccttac aacatttaat tgaaggcaaa gaactaccca
     2041 gagaagacat gattaatatc atgcggggaa tcatgggtgg tgagcttcct gggccaatgg
     2101 ttgctgctat cttggttgcc ctgcgcagta aaaaagaatc ccctgctgaa attgctgccg
     2161 cagcacaagt catgcgtgac tttgctacac cagtaagtgt tggcgataag agccacctag
     2221 tggatgtagt tggcacgggt ggcgatggcg cacatacctt caatatctca accgcatcga
     2281 tgtttgttgc atgtgccgcc ggagcaaaaa ttgcgaagca tggtaatcgt ggggtaagca
     2341 gtaaatccgg tagtgctgat gtcttagaag ctcttggtgt gaaactaaat ctatcagccg
     2401 aggcagttgg tcgttgtatt gagcaaattg ggctaggttt tatgtttgcc cctaaccacc
     2461 acccagccat gaaaaacgtt gtccccatcc gcaaagatct gggcgtacga accatcttta
     2521 acatcctagg tccacttact aatccagcca aggcacctaa tattttgatg ggcgtattta
     2581 actcagagct tgttggtatt caagcaaaag tattacaact cttgggggcc gagcatgcct
     2641 tagtagtgca tggcaaagat ggtcttgatg aaattacgct gtcggggcca actgtcattg
     2701 ctgagcttct aaatggtcag attaaagaat atgagatctc ccctagtgat tttggaatgg
     2761 caaccgcctc tctcgaatcc ctcaaggtga aagatgctaa tgagtccaaa gcgatcatct
     2821 tgcaagttct taataaacaa tcaggccctg ctgctgatat tgtttctata aatgcaggct
     2881 caacacttta tgctgcgaat attgcgccca gtattcaagt gggtgttgag atggctaaag
     2941 atgccattgc ttccggtaag gcgaagcaaa agttagacga gctcattact ctctcgcaaa
     3001 ccttggcagg ataatcattc catgagcgat attctttctc ggatcctaga taccaagaaa
     3061 caggaaatta gtgctgcaaa gcacttagtc tctagtgacg cattgcttca acaagcgatt
     3121 cagaacaatc tggataagaa tctaaagcca cggggctttt ctaggtctct cattaatacg
     3181 gtagcaagcg gtaagcctgg catcattgcg gaaattaaaa aggcaagccc tagtaaaggn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcatggcgc tacctgctta tcagtcttaa
     3361 ccgataaaga ttattttatg ggctcacccg agtttctcaa agaagctaga gctgcttgct
     3421 ctctaccagt gattcgcaag gatttcatca ttgatcccta tcaggt
//
LOCUS       DDPQ01000033            7226 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_8112,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000033 DDPQ01000000
VERSION     DDPQ01000033.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_8112"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1364..1439
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1829..1868
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6633..6681
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1904 a         1738 c         1749 g         1666 t
ORIGIN      
        1 catacccaaa gagcgaagga gcgtctgcgg gtccgagact ttgatgatct cgaactcggt
       61 gtcgcacaaa tgatcgcaga cccacgcatc gcagcttact tacaagcgcg tttggatgcg
      121 cgctacaaac atattttgat cgatgagttt caggatacca atccattgca atggcaaatc
      181 ctcaaatcat ggctagaggc ttatggtgat gatgctaatc gacccaaggt atttattgtg
      241 ggcgatccga agcaatcgat ctatcgcttt cgtcgtgctg acccacgttt gtttacagca
      301 gccacacatt tcttagaaaa gcactacggc gctgcactgg aagaacaaaa taccacccga
      361 cgcaatgccc cgaagattgt ggaagcggtc aacgccatct ttgctgcaga ccgtataccc
      421 gagtcctatc catttaaaga gcaagatact cattggaccg caccgtcatt gccagagcca
      481 gctgtttatc aaagcggcga ggcttatcgc ttgaacctta ttccctatga ggagccatca
      541 gaagatcaac gctttggcaa tgccctcgag gcagctttgg ttgatcctca cgagacccct
      601 gcaaaaaaac agcgtgcgca agaagggcga agaattgcga gccttattac gcatctaatc
      661 caaactcgac gggtcttaga cgaagagctc gacacaagta atagcaaggt aaagcgcatc
      721 gtctggcgat cagcacgacc aggagatttc ttactattag taaagcgccg tgcttactta
      781 tcagagtatg agacagcact acgtgacgct ggacttctct atgagagccc gcgcttaggt
      841 ggattgctag agacccttga ggtcgatgat ctaattgcct tacttactat attagtaact
      901 cctggtaatg atctcgcttt ggcacaggtc ttacgtagtc cactatttgc gctcgatgaa
      961 acacaaatgc aaaccttggc tgagttggta caaagtaatc agggttaccg caattggtgg
     1021 gaggcactga cccatggtga gagccattcc catcaggcgc aattccaaag cattacccaa
     1081 cacatcaagc aatggattga tctagccaaa cacctaccgg ttcatgatct gctcgatcat
     1141 gtgtatttct cgggggatgt ccggcttaaa tacgcgagtc attgtcatga gagtgatcgc
     1201 gatcaggtat tagccaatct agatgccttc ttagagctag cgcttaatct tgatggcggt
     1261 cgctacccaa gcttgggacg ttttattact caactgaatc ggatgcgacg cggcgatgaa
     1321 gacgagaccc cagacgaagg ggagatgagc gcagaggctc agannnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1441 aggcaccctt tgtgatcttg ctcgatagta ataacactgc tgcacctcat tttggaagtg
     1501 gcgtcttact cgattgggat ccagagcaag ccggaccgag tcatttatcc atgttcacca
     1561 aactcagtct gggtgcagga cgcgaagacc tcaagcaaca agaaaaagag attgccctga
     1621 aagagaactg gaacctactc tatgtagcaa taaccagagc taagcaaggt ttttggatga
     1681 gtggcgtgga gagctcgcag aatcgagagg acgctggggt gagcaaagat agttggtacg
     1741 cacgcgcacg cagcgcagag cttcccctct ttaccgagct cgatgacgat ctgcagtcat
     1801 cgattcggat acaaaagcac atagcaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnngg gtgatgaact agttgggggt gatccgctca gcattagccc cgagcgacaa
     1921 caggctttgg atgagggggt atggtttcat ggggtattgc aacgcatcag cccacaacaa
     1981 tatcgaccgg tctctcacac cattccggag cctaaagcga ttgcaagtgc attcatgatc
     2041 tcgcttgcag atgcagagcg cgccttacag cgcgccatca cggtttacca agcacctgag
     2101 ctacagactt attttgatcc cacgcaatat caggaagcgt ggaatgagct cgatctggtg
     2161 agcgccgaag gtaaaagtat gcgcattgat cgtctagtnn nngatgatcc gctctaccaa
     2221 caatacacag aacaattaac aaactatcaa gtcgaggtgc aacgtattta tccacataag
     2281 gctattaagg cgatcctaat tgatgcccaa ggaaaaagtt tgccgctaat tcactagcag
     2341 caactagatc tcaacaatca tctcaatctc cacacaggct cccaagggaa tctgtgcaac
     2401 tccaaatgcg ctgcgagcat gcttgcccgc atcaccaaag atctcaccaa gaagctcaga
     2461 gcaaccattg accactaggt gatgttctgt gaactcggcg gtagagttca cgagacccat
     2521 gagcttcaca attcgcttga cgcgatctaa ggagcctaaa tgggcattaa gggttgagag
     2581 aagatcaatg gcaatactgc gagccgctgc cttacccctt gcggtatcca tatcaagacc
     2641 cagcttacca acccaaggct tgccatcttg tttggcaata tgacctgaca gaaagacctg
     2701 attgccgctg gtaactgcca tcacgtaggc ggcagcaggg gtgccagggg tgctgagggt
     2761 gattccgagt ttggataatt gttgggcaat agcgctcatc agtacctttc aaataagtgg
     2821 gggaataagg gtgcgattag agcttgcgct cagacaccca tcagtataag aaattggcaa
     2881 aaatacctga caaattccct ctccgctata cttcagccta caaaatatta accatgttgc
     2941 aatacgctta agcatatgaa caaagccatc attttctcaa tcggcacagc actaattgct
     3001 agcctatttt ctattaatgc ccaagctcaa gacgtcaagg gtactgccca agccggtcaa
     3061 gccaaagtat ggatctgcgt aggttgccat gcgatccctg aataccgtgc cgattggcca
     3121 caggtttacc gagtgcctaa attgggtgga cagaacgctg agtacattat tgcttcctta
     3181 aaagcctaca agtcagggga gcgcaagcat cccaccatgc gcggaattgc tggcagcctg
     3241 agcgatcaag atatggctga cattgctgcc tactatgctg cgcagaatgc gaactcaccc
     3301 cgtaacccat taaagtaatt ggaatagaga ccatgattaa atccgctctt attcttgcct
     3361 tactcagcgc ctctgtagga attgcgcatg cagccgatat tgccaaaggt aaagaactcg
     3421 ttgaaaagaa caactgtgca gcttgccacg gtgctggctt aaaggcgcct gtagcagctg
     3481 cgtatccaaa gattgctggc cagtaccaag actatttgta ctacgcattg aagtcttaca
     3541 ctgtaaacaa tgcgaatgtt ggtcgaaaca atgcgatcat gcaatcccaa atgaagccat
     3601 atagcgataa agatctacgt gatatggctg cctacatcgc ttcattacca agcgacctag
     3661 tggtcaagaa ataagttcca gacttattag cagaaagccc cgcaagtcgg ggctttttat
     3721 attctgactt ctatttagca gcctctaagc ctcgggctag atgcgtgcgc atggcttgct
     3781 cagccgcaat ggggtcacgc ttgagaaggg ctttcagaat ctcacgatgc tccgacaagg
     3841 aggattgcaa acgacctgaa cgacttaacg agtccttgcg ctgtaacttc aatactttgc
     3901 gaagatccgc aatcacactg gtcatccatg gattattggc aatagcaatg atgcgttcat
     3961 gaaaccgtac attaatctca aagaactgat ctagattgcg atcggccgca gccttctcta
     4021 aacgcaagtg catgtcatcc aagtcattga gatctttctc cttcgctttg ctggcagcct
     4081 ctttggcggc ttcaccctct aatagggaaa ggatcgtaaa tatttgctct aaatcacccc
     4141 gattaacctc ggtgacataa gcacccctgc gcagcttcat ggtgatgagc ccctcggccg
     4201 ccaagacctt aatggcctcc cgcaggggag tgcggctaat cccaaactct ttagccagac
     4261 tttgttcgtc aatccaggcg ccaggagcca gatcatgggc aaagatccgt acccgtagct
     4321 ggtctgcaac ctcttcgtag agcggcctag atgcgagtat cttactcata ttaatctctt
     4381 aaggtactgt ttataaacat ttttatctaa aatgatcaat attctgaatt cataattatg
     4441 tatccaattc tatagacaaa tggattaaag tcaagcaaaa tacctgtatt cctacccatt
     4501 gatggaaagc acatgagtca gaaatcccca aaggctagag cagtctggcc agctgcccca
     4561 gcccccaatc aggaggcttg gcaagaagca gccagcaaat cagcaccaaa cggtaatgtc
     4621 gatggcctcg gctgggagac cccggacggg atccatttaa aggccttgta tacccaagcc
     4681 gatttggctg gagtgccgta taccaatagc ctgccagggt ttgagcctta tctgcggggc
     4741 ccgcaggcaa ccatgtattc cgttcgtcct tggaccattc gtcaatacgc agggttctcc
     4801 accgccgaag agtccaatct cttttatcgc aaagcattag agggcggcgg tcaaggtata
     4861 tccgtagcat ttgacctcgc cacacatcgt ggttacgact cggatcatcc tcgggttgtg
     4921 ggtgatgtcg gtaaagcagg tgtggcaatt gattcagtgg aagacatgaa gatattgttt
     4981 gatggcatac cgctagacaa ggtctcagtc tcgatgacca tgaacggtgc tgttctccct
     5041 gtacttgctg gttacattgt ggccggtgaa gagcagggtg ttacgcaagc ccaattaagc
     5101 ggcacgattc agaacgatat tctgaaagag ttcatggttc gtaataccta catctatcca
     5161 cccgcaccat cgatgcgcat cgttggcgac atcattgagt acaccgccaa gaacatgcct
     5221 aagttcaact cgatctccat ctcgggttat cacatgcaag aggctggcgc taatcaagca
     5281 ctcgaacttg cattcacact ggccgatggt aaggagtatg tcaagaccgc attagcaaaa
     5341 ggtcttgatg tggatgcgtt tgctggccgc ctttcattct tctttgcaat cgggatgaac
     5401 ttctatctag aggtagccaa actacgagct gcgcgtatgt tgtggtggcg catcatgaaa
     5461 gagttcaatc ctaagaaccc caagtcatcg atgttgcgca cgcattgcca aacctcggga
     5521 tggtccttga ccgagcaaga cccctataac aatattgtgc gcaccactgt agaagcgatg
     5581 gctgctgtat ttggtggaac ccaatccttg cacaccaact cctttgacga agcaattgcc
     5641 ttgccatcgg agacctcatc gcgcattgcg cggaatacgc aattgatttt gcaagaagag
     5701 acccatatca ccagcgtggt tgatccatgg gcaggttctt acatgatgga gaagcttact
     5761 caagagatgg ctgatgcggc ctgggaaatt attgccgaag tcgatgcaat gggtggcatg
     5821 accaaagcgg tagaaagcgg ttgggccaaa ctcaagatcg aatcagcagc tgcccaaaag
     5881 caagccaaga ttgatgctgg tgcagatgtg atcgtcggtg tcaataaata caaactacag
     5941 gaagagagtt tggtagatgt cttggtgatc gataacgata aggtacgcga taaccagatc
     6001 gctcgcctga aatcgattaa agctaaacgc gatcctaaaa aagtacagga agcactcgct
     6061 gcactatctc aagcggcaga acagaacaca ggtaatttat tagatctagc gatcaaagca
     6121 atccgtttac gcgcaacggt gggcgaggtg tcggatgctt tggagaacgt ttatggccgc
     6181 caccgtgccg atacacaaaa ggtgaccgga gtgtacgcag ccgcatacga ttcagcagag
     6241 ggttgggaga agctcaaagg cgagatcgcc gagtttgcaa aacaatatgg ccgtcgccca
     6301 cgagtgatga ttgccaaact aggccaagac ggtcatgacc gtggtgcaaa agtggtagca
     6361 accgcctttg cagatctggg ctttgatgtg gatatgggac ccttgttcca aacacccgaa
     6421 gagtgcgcgc gccaagcaat tgagaacgat gtgcatgcac tcggtatctc gaccttggca
     6481 gccggacata aaacactggt gccagcgatt attcaggagt tacgcaagca aggtgccgat
     6541 gacatcatcg tctttgtagg cggtgtgata ccaaggcagg attatgagtt cttgtatgag
     6601 tcaggggtca aagggattta tggaccaggc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn ncaaaccgat gagcaactga ttgcagcttt gttgggcaaa
     6721 ccgtgcccag cacagcgccg tgcgcttgct aaagcgatta ctctcttaga gtccacccgc
     6781 accgatcatc gtgagcgtgc cgatacattg ctcaatacga tcttgcccca taccggcaaa
     6841 tcattccgtt tggggatctc tggagtgccg ggtgtgggta aatcaactct gatcgagaac
     6901 cttgggctct acttaattaa acaaggtcat cgggttgcag tgctagcaat agacccatcc
     6961 tcgagtttgt cgggtggatc aatcttgggc gataagaccc gcatggagct tctctctgtg
     7021 aacgagaacg cattcattcg tcctagccct tcatccggaa cactcggtgg tgtcgcagag
     7081 cgtactcgcg aggtgatgct cttagcagaa gccgcaggcc atgatgtcgt gattgtggag
     7141 accgtaggag ttggccaaag cgagattgcg gttgcaggca tgaccgattt gttcatgttg
     7201 ttgcaattac ccaatgctgg tgatga
//
LOCUS       DDPQ01000034            2545 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_51006,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000034 DDPQ01000000
VERSION     DDPQ01000034.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_51006"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    762..823
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          668 a          589 c          598 g          628 t
ORIGIN      
        1 atggagaata tcttgaccca agccgctagt atcaacacct cagttgtaag ccaaacattt
       61 aacaacacca tttccgctgg tagcaaagca atcaataccg tcatcgctac tttgcgtatc
      121 attgcatcag catgggaaga gtcccgtgcg gaatataaaa aacatgccat cttaggtggc
      181 ggctgggaat aattgaaaac ggttcgcatt gatttcgtat ctgacatcgc ctgcccatgg
      241 tgtgccattg gtttggcatc cctagaaaag gctttggatg atgttggtag cgaagtgaat
      301 gttgaagtct actttcaacc ctttgagtta aatgctcata tgcccctcgg tgggcaagat
      361 gtgattgagt accttaccga gaaatacggc agcactgtta gtcaagtaaa agctaatcaa
      421 gagaatattc ggaaacgtgc cgctgagatg ggctttctgt ttcatccgga agggcgcaag
      481 cgcgtgtaca acacctttca tgcccaccgc ctcttgcact gggcgcaagc tgagcacggg
      541 cttgatgccc aatacaagct caagaaagaa ctcttcgcaa cgtatttcac cttagcggtc
      601 agcgttgatg atcctaataa tctgctggct gcggttgagc gtgcaggatt accgagtgag
      661 cgtgctcgag ctattctgga aagtgatgaa tattcgcaag cggtcaagga tcaaaagaag
      721 aagtacaccg agttgggtat taactcagta ccagctatca tnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgcgcg attgcgccca
      841 agaatcaaat cattagcaag tccgttaatc gacttactct ttgatgttgt tctgaatcac
      901 aaacttaccc caaataccaa tctgctttgc cacaaatgca ttggctgctt ctgggttcaa
      961 gctttgcacc tgaatgcctt gggtctttag cttctcggcc acagcagggg tcttcaaagc
     1021 cttatcaacc gcacgataca ttgcatttac aacagcaggc ggggtctttg atggggcaat
     1081 cacggcccac caagcgggtg cctcaaagcc tgggtatcca ctctctgcca aggtaggaac
     1141 ctttggcata tcgggtgagc gctttgcagt ggtcaccgcc aaaggaatca agccgccact
     1201 atcagcatgc ggcttaatta agaacatact gccaaccact agaggaactt gaccagcaag
     1261 accatcctga atcattgggc cgcctccacg atagggggca tgcacccaat caacatctgt
     1321 tttcttggca aagtcagcaa ttgcaagatg gcctaagcta ccgataccaa tagagccata
     1381 agtaaatggt ttcttcgcct tagagtcagc cattaactgt ttcatgctgg taatactaga
     1441 tttcttgctg gctgctaaga ccatgggtga agttccaatc atattgaccg tgaccaaatc
     1501 tttgagcgtg tcatagggca ggttatcttt aagagatgga ttaacgccat gtgtatcaaa
     1561 taccagacca aaggtgtagc catcgccagt ggctctagca accgcggtag taccaatcac
     1621 acccgacgcg ccaccaatat tctccacaat cacattttgc ttaagctccg cttgcaaaac
     1681 cggtgccaat atacgagcaa cttgatccac cgatccaccc ggaggaaaga ccgcaataat
     1741 tttgatgggg cgttggcttg gccaattacc tacatcactc tgggcaattg caggagtaat
     1801 cacgcaaagg gcaaacaaga cagaaacgat ctttttcatg agggtaggca taacggctcc
     1861 tttaagtagt gctgattatc atactggaac ttacccattg atcctaagca atcagcaaag
     1921 caatacaatt gcctaaactc cagcaaagga ttgtattgat gagccatggt attcatattt
     1981 ccccccgtgt tgaacctgct accgctggta tcaatgcctt gcgaggcata cccagcacag
     2041 tcattagtga tatgttgggg cgcaccttag tggctaatgg gatccaaccc attcatcgct
     2101 ctcccatgtc tatatgcggt aatgcattca cgatcaaact acatgttgct gataatttaa
     2161 tggtccacaa agcactgcaa atggtgcaag cgggcgatgt gatggtgatt gatgtggagg
     2221 gtgatactgg atgcgcagtc attggcgaga tcttaatgac cgtcgctaag agtaggggcg
     2281 tgcttggtat tgtggttgat ggggctgtgc gcgatgtgga tgcctttgat acaaattcat
     2341 ttccctgttg ggccaagggg gtcaacttgc gtggtcccct caaagaagga ccgggatcca
     2401 ttaatgtacc gatctctatt ggtggaatga ttgttcatcc aggcgatatt attttggctg
     2461 atagcgatgg cgtcattgca gtgccgccta gccaggcatt agaggcagca cgactcggtc
     2521 aggaaaaagt taaacaagag caaga
//
LOCUS       DDPQ01000035            4795 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_15195,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000035 DDPQ01000000
VERSION     DDPQ01000035.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_15195"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1151..1184
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1309 a         1173 c         1071 g         1208 t
ORIGIN      
        1 accgcatagc gcgtcataaa ctgctctaca gtcacaaagc gcatatcgtg atgctgggtt
       61 ctgcctagta aatcatataa gaccccgacg cgcaatgccg catcactgac ctccatacgt
      121 tcaatcccca gctcatcaaa gatggccaac ataatcgaga gcccccctgg taatacggga
      181 cgtcgatcct cttttaaatt aatgagggtg acccggtcaa tatgatcaaa atcgagcagg
      241 cggtgcttga gaccctttag accctgcagg gtaatgaccc cgtctaatcc agaactagtc
      301 gaatcttgac cattcttttc ctcggggcca tttagcttgt tatcggcaat caaatcagct
      361 aaggctctgg ccgttccaga agagccaatc acttgattcc agcccgattt atgaaagcgt
      421 cctttaatga cttgcacctc gcggcgggca gccagctcgg cttccttaaa ggcatgcgaa
      481 tcaatcgcac cattcggaaa gaagcgaatg ctgtgcgata cacagccaat ataaagactt
      541 tccaggagct tcggttcata tcctttgccg atgatgaact cacttgatcc accacccaca
      601 tcaatcacta aacgattacc ggagactgca ggtgcctcat gcgaggtgcc aatataaatg
      661 aggcgagcct cttcaacgcc agcgattacc tcgattggaa atcccagcgc aatttgcgct
      721 tcttgcacaa acttttgggc atttctggcg acccgcagcg tatttgtagc aactgctctt
      781 acctgctcag gacgaaatcc ccgcaagcgt tcaccaaagc gcaagatggc ctctaaaccc
      841 cgtttgatcg cctcatcatc caagaccttc ccgggggtta ggccagccgc tagtcgaact
      901 ggctctctca gcgtatcaat ggtccgcagt tgcatccctg agggggtgat ataggcctgt
      961 gaaatcaata agcgaaagct gttcgaccct aaatcaactg cggcgacaag gtctaggcca
     1021 ggtttgttgg ggggtatcgg tttagacaag gtagtagagt gttagcatct taataaccct
     1081 attatcgtat gaaataaaag tgacatattt atgaaatact acaagttatg tcacttttat
     1141 tactaaaccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaagat tccgcttcta
     1201 gagcgtatcc gctttctcag tattgtctcg agcaatctcg atgagttctt tgaaatccgc
     1261 atggcaggta ttaaagagca actgatcgat aaccccgtta aagtaggttc cgatggcaga
     1321 accattgcgc agagttacga tcttgtatcg caacaagccc atgcactagt agaccggcaa
     1381 tacaaactct accaacaggt actgctaccg aatctgaaga aggctggtat tgaatttttg
     1441 ttaacggaac attggtctag tgcccaacga tcgtgggcgg aagatttctt caaagaagaa
     1501 ttattacccc tactaacccc gattgctcta gacccagttc acccgttccc ccgagtaatc
     1561 aataagagtt tgaactttgt ggtgcagctg catggaaatg atgcctttgg acgcaaggca
     1621 aacctagcgg tagtccaagc gccgcgcgct ctacctcgtc ttgtaaaaat gcccaaacgc
     1681 ttatgtggcg ataccgaggc attcgtattt ttatcctcat tcattcaggc cttcgtatat
     1741 caattatttc cgggaatgga agtgcttggt tgttatcagt tccgggtcac ccgaaactca
     1801 gatttgtttg tgtcagatga tgaggttacc gacctccgta aggctttaca aggtgagcta
     1861 ccttcgcgtc atttgggcga tgctgttcgt ttggaagcca atattaatat tccggatgat
     1921 ctattgcaac ggctatgcaa agaaaacaat cttgatttgc gagactgcta ccgggtcaat
     1981 ggtccggtga acttggtgcg cttagcccag ttacccgatc tagttgataa gcccaaatta
     2041 atctttacac cctatgtgcc cgcgttcccc ttcaaacacc atcacaatac gtttgcaaat
     2101 tcttattttg agcgaattaa gcgcgctgat atcttgttgc atcatccgta tgagagtttt
     2161 gagcccgttc tagagctctt gcgtgaagca gccaaagatc cggatgtctt agcgattaaa
     2221 caaacagtct atcgcacggg agataaatcc cccgtcatgg aggcgctcat cgaggctgca
     2281 caaaatggta aagaggttac tgttgttctt gaactcttag cccgctttga tgagcaaacc
     2341 aatattaact gggcagccaa gctagaggaa gttggtgcgc acgttgtcta tggtgtggtg
     2401 ggtcataaat gccacgccaa gatgctgtta attgttcgta aagagaaaat caataaatcg
     2461 ggtaagacca gtttggtgcg ttatgctcat ttgggtaccg gtaactacca cccccgtact
     2521 gccaagctct ataccgactt tggcttaatg acctgcgatc cccacctcac aaaagatatc
     2581 catcacgtct ttcagcagct aacaggcact gggatgcaac tggaaacccg agagatttgg
     2641 gaggcgccct ttacgatgct tgagcaactt gttcatcata ttcgtattga gaccaaggct
     2701 gcaaaacaag gtaaaaaggc gcgcatcatt ggaaaaatga atgccctcct tgagcccacc
     2761 atcatagaag agttatataa agcctcccag gcaggcgtgc aaattgatct cattgtgcgt
     2821 ggcgtctgcg ccttgcgccc tggagtcaaa ggtctttctg agaatattaa agtgcgctcc
     2881 attgtgggtc gctttttaga gcaccatcgt atttattatt tctatgccgg tggcgacgag
     2941 gtcttatacc tctccagcgc cgattggatg gaacgcaatc tattgcgccg agttgaagtt
     3001 gcctttccgg taaaagatat caaactcaag caacgggtta tccaagaagg tttaatgata
     3061 ttgctcaaag acaatgcctc tgcttggctg atgaaagccg atggcagtta tgtcatgagc
     3121 aaaacccgac tcaatcaaga accgcttatc ggacagattg aattacttaa gaagttctcg
     3181 cgggtggaaa gcgcaaggta aaagtgctac cccgaccggg ggtactctca atcaccaaat
     3241 tggcttgatg gcgattggca acatgcttca caatggccag tcccaaacca gtaccgccag
     3301 tttctcgcga ccgactgcga tcgattcgat aaaagcgctc agttaagcgc ggaatatgct
     3361 caggtgatat gccaggacca gtatccgtga ccgaaaattc accttcgcca gcagcattga
     3421 ccgaccacgt cacgctcaca gaaccaacat tcggtgtata acgaatggca ttggagacca
     3481 aattactaaa agccgacaag acctcgcgtt cctctcctaa taaattacag ggggtcttaa
     3541 gatcaaagtt aagggagtgc tttccttgag atagcgcata ggcatcgttt ttgatcaacg
     3601 acatcaaata actcattgaa atgctattca tgggggcggg ttgtgtgttg gcctctagat
     3661 tagctagggt caacaaatcc tccactaagt ttttcatcct tttaccctga tccatcatca
     3721 tgtcaaggta ttgagctttc tgctcagaag gtaactctag ggtttgaact gtttctaaaa
     3781 aacccatcat gactgttaat ggcgtgcgca tctcatgcga cacattcgcc acgaaatcac
     3841 ggcgcatggc ttctgcctta ctgagatcgg taatatcctg aataagaatt aggcgtcgat
     3901 tttcagagaa tgggaacact tgcagtcgca aaatgaggct tgaggcactg cccatttttt
     3961 caagaacaac gggttcctcg tactgacggc tcatcatata ctggacaaat tcgggcttac
     4021 gcacgatgta atgaattcgt tggagagcat cgcgcttgaa ctgaagcccc aaaaactgtt
     4081 cagcaatcgc attacaccac tcgatttgat cttggtcatc cagcatcaca atgccattgg
     4141 gggatgcctg aaatgcctca ataaatcgtt tatgttggcg ctctatctgt tcaatatcct
     4201 tgcgcagacc tgtaatgatg cgttgtaaac gaaagaaaat ttctgaccag gcaggtccaa
     4261 agccagacaa gcttgaaacc cctgcccctt tggcaaacac atcaagacgc tcttggttga
     4321 tgtatgccga taaaaggcta taagccaaga tagaaaaggc aatcaaccac cctgctatct
     4381 cacccagcaa agatcgccca ataacaccag caatgccggc aaccaaaaac atggtgatga
     4441 gacgaataaa agcgtagaac atggcgacat cttagagcat cggagaacga tgctcaaaat
     4501 ttaggcagct tacttaacta ggagtcttag taatccgata cccactgcca cgaacagtct
     4561 caatataggg atcgcattcc gcttcagcca atgccgctct taagcgcttg atgtgcacat
     4621 ccaccgtacg ctcttcgata taaacttgat cgccccacac tcgatctaag agatgcgagc
     4681 gcgaatggac acgctcagga ttactcatga agaattgcaa taagcgaaat tcggttggcc
     4741 ccaaattaac aaactgatcg ctttgatttt ggcgagatac ggtaactcga tgggt
//
LOCUS       DDPQ01000036           17886 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2282,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000036 DDPQ01000000
VERSION     DDPQ01000036.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 17886)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17886)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17886
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2282"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2670..2715
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4523..4564
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14740..14827
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4630 a         3946 c         4248 g         4886 t
ORIGIN      
        1 cctccatggc cctatctctg gttttagtaa ttgcgattat gcaatcagcc acgcagggag
       61 ttactgtagg aggtttcgca gcttttatta ccgcgatgct cttaatcatt tcaccactaa
      121 agcatcttgc tgacattaat caacctttgc agcgtggctt aactgcagca gagatgattt
      181 ttcaattgat tgatcagccg attgaagaag aagacttgag tcagtcaaag ctcaaacgat
      241 tagataaggc caagggagaa atccagtttg agagcctatc cttttcgtat gaccaagaag
      301 agggtcgtaa agatgctctt cgcaatgtca atcttgatat caagccaggc caagtaattg
      361 ccttcgtagg gccctctgga gggggtaaat caaccctagt gaatttattg ccacgtttct
      421 ttaaacctaa ggcgggccgc atcctacttg atcaaattcc gattgaggaa attatgttgg
      481 ctgatttacg taagcaaatc gcctttgtaa gtcaggatgt cattttgttt aatgacacca
      541 ttgcggccaa tgtggcatat ggctcaagtt ctgaggggat tgatcgcggt cgtgtcatcg
      601 aagctttgga ggcggctaat ttaacaggac tcttaacgga gctgcccaat ggtatcgata
      661 ctttaattgg ggataacggt aatcgtttat cgggggggca gcgccaacgt ttagcgattg
      721 caagggctat ttataaggac gcgcccatcc tcatactcga tgaggcaacc tctgcactgg
      781 attctgagtc ggagcggcag gtacaagaag cgcttgatcg tttaatggca gggcgtacaa
      841 ccctggtgat tgcacaccgt ttatcaacca ttgagcatgc cgatcggatt gcggtcttgg
      901 atcatggcga gattgtggaa tatggctctc accaagaatt gatggccaaa aatggcctat
      961 atgctggttt acatcgcttg cagtttagtg aagcaaattc ttaagagtgc tcattagtta
     1021 agaacaatat cgtattgttc ttgtttgaag ccagcctctg cttgaagccg aattggcttc
     1081 tgaacaaaat cccctagcat cgctagaaat tggttctctt cttctaaaaa tagatcaatt
     1141 acatcgggtg atgccacaat ccgaaattct ttcggattga attgtcgatg ctccctcaca
     1201 atctctcgta agatctcgta acagactgtt tggggtgtct ttatctcacc cttaccagta
     1261 cacgaggcac aaggttcaca caaaatatgg gataaagatt cgcgggtgcg tttacgggtc
     1321 atctcgatga gccctagtga tgagaagtca ttcacatttg tgcgcacatg atctctagca
     1381 aggtttttct taagctcatt gagaacagcc tcctgatgat cggtctggct catgtcaatg
     1441 aaatcaataa tgatgatgcc cccaagattg cgtaagcgta gttggcgtgc aatcgcctga
     1501 gctgcttcta agttagtctt gaaaaccgta tcgtccaaat tacgagcacc cacaaagcta
     1561 ccagtgttaa cgtcaatcgt ggtcatggac tctgtttggt caatcataag atagccgccg
     1621 gatttaaggt ctacgcgcct tccaagcgcc ttgctgatct cagattcaat atcaaataga
     1681 tcaaagaggg cgcgctcacc ccgatataaa actaatttat tgtttagttg cggggtaaat
     1741 gtttgggcaa attggcctaa gcgctcaaag ttctcggcag agtctacgcg gatttgagta
     1801 gtttcagggc cagccatatc gcggagaacc cttttggcaa gacttaaatc ttgatgcaat
     1861 agagtaggag cagctttttc tttcgatgcc tgcaaaattc cttgccatgt ggtgcgtagg
     1921 tacttgaggt cagcaatcag agtttcatcg ctagcaagcc cagcgctcgt tcgcacaata
     1981 atatcgccgc tttcatcagc agggattaga ttctttaacc tattttttaa ggcctcgcgc
     2041 tcttcaggca tctcaatttt ttgggagatg ccgatatgga tttcagtccc aactgcacga
     2101 tgatcgtgcg gtaaatagac caaattgcgc cccgcaatgc taatttggcg agtaagccga
     2161 gcacctttgg tacccaaggg atcttttaga acctgcacta gcagggtctg cccctcgaat
     2221 aagattcgct cgatttgccc tcctgcatga tgatctccca agtcattgaa atgaataaat
     2281 gctgcacgat ctaatccaat atcaatgaag gcagattgca taccaggcaa tactcgcact
     2341 accttggcta ggtaaatatt actaacgatc ccccgttgtc gagtgcgctc aatatgcagt
     2401 tcttggaccg cattttgttc gatgatcgca acccgagttt cttggggggt gatattaatt
     2461 aggatctctt ggctcatatg agttcaaacc aaaatgcaca tgggcttgtc ttaaaagttt
     2521 tgcggtctca taaaggggaa gccccatgat accgctataa ctgccctgga ttgttttaat
     2581 aaaggaggcg gcaattcctt ggataccata ggatcctgct ttgccaaagg cttcaccgct
     2641 atgtacgtaa gactcgatcg ttgccgtatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnatcga tagtaatggc tatagcgcta tacacatcat ggcttttacc
     2761 actcagttga tgaagcattt gggtggcgtg agcggcatct gttggcttac ctaaaatttc
     2821 agtatcgaga gatccgggca ggctcactgt ggtgtctgca cataaaatcg gcgcccaagt
     2881 aaattgcgga ttattttgtt gtagtgcttg ccaacgatct aaagccgctt cactttttgc
     2941 gagtgtgact cgtcgaacat agtcaatagc tttctcatgg gctaggggta tttctagaga
     3001 ttctggattc tcattacggc tgggtaggag taatttaaag tgaaccccaa tttgtgccag
     3061 taactcttgg cgacgggggc tttgtgaggc cagataaatg aagtcgtatt tcatcgaatt
     3121 aatctcttat tctcgatggt aggggtgacc ttgtaagatt gtccaagcgc gatagagttg
     3181 ttcgattaga accagccgtg caaaagcatg aggaagggta agactagaga gtcgccagat
     3241 aggcatctct ttggttttaa agctagcatc tagcccatta gctccgccaa ttaaaaagac
     3301 aatgtctttg ccaacctccc gccattgttt taattgccca gcaaggtctt gggtgcttag
     3361 atcttttcca tgttcatcta gagcaattac tagagtattt ttggggatcg ctgcaaggat
     3421 tttgaccgcc tctttacttg ggctgatatc tggcttcaat tcctttagat ccaccgcaca
     3481 atctggcggc atccgcttgc tgtattcctg gtaggccatg ctgacccaat caggcatttt
     3541 atgacctact gcaataatca gtaaacgcat gtgctagtcg tcttcaggct cttcgcttgc
     3601 tttgacgagt tttttgggac cacttaactt cacgcgcacg ggtttatcac cccagatacc
     3661 ttcgagttga tagtaggagc gtagcatcgg ttgcaaaata tgcactacga tatcgccgca
     3721 atcaacgagg acccattcac cggtttctag accttcgata gagataacct cacccccttt
     3781 gtctttaaca ctttccttga ctgagaaagc gagcgagcgt gtttgacgat tgctacttcc
     3841 agttgcaatc acaacccgat caaatagctc gctgagtttg ctggtgtcat acacacggat
     3901 gtcttgtgct ttgacgtcct caagagcatc aatgatggtg cgttgtaatt tacgaatatc
     3961 catatttacc gctatcttac cttcttgact ggtaaagacc ctttttgagg atgagagcat
     4021 gtatagattt agggagccat tcttgaatac gattagcggt attgttagag gaaagtaaag
     4081 gacgcaggct gcttgaggca agatcaatcg acagttgctc atctagataa attaacccag
     4141 aggctttaga acaaagatca tgggctaact tagtttgatg gtctaaatag taggcttgaa
     4201 gaggtggatg atctatcagt tgtttctgaa tcgagtaggg tggccggcta gcaaccgcca
     4261 aatgaatgta tcgactaagg tctcgccaat cgttccaggt atagagttgc aggaaactat
     4321 ctgcacccat taaccaaatt agggagacat caggaccaaa tttttggcgt agctctttgg
     4381 cgctatcaat cgtatagctc gggccaccgc gatcaatttc catcgaatct accatcacct
     4441 tagtgtcgat attctttgct tgcaactctt ccttaagcac atccgctgcc gcaattgtca
     4501 tgtcaagccg gtcttttgca ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnntcttaa ggctcggaga gccaatcgcg gggcttgagg tagcgctcat acaagcgtgc
     4621 ctcaggtgtc ccagcatgag gttgccagtt atatttccag gtgactacgg gcggcattga
     4681 catcaaaatc gattcagaac gacctcctga ctgcaaacca aaaatggtgc cacggtcaaa
     4741 gattaggttg tattccacat aacgaccgcg acgatattcc tgaaaatcac gctccgcttg
     4801 ggtatagggc tcttgatagc gacgctctag aatcggtaag taggccttga tcaaagagtc
     4861 gccaaccgat cgcatcatcg caaaactttt ctcgaaccct agttcgttaa agtcatcaaa
     4921 gaatattccg ccaataccgc ggggttcatc gcggtgtttg agatagaagt actcatcgca
     4981 ccatttctta aaacgagggt acagaccagc gccaaatggg tcgagagcat ctttggctgt
     5041 ttgatgaaaa tgtttacagt cttcgtccac gccatagtag ggagtcagat caaaaccgcc
     5101 accaaaccac caaactggtt cttgattcgg agcttgggca ataaagcaac gaacattcat
     5161 atgggtggtt ggtgcctttg ggttgcgcgg atgaaacact aaagagacgc ccattgcctc
     5221 aaaggttcga cctgcaatct caggacgatt ttgggtggca gtaggaggta attgattacc
     5281 gctgacgtga gagaacccaa caccgccttt ttcaagaaca ttaccattct ctaaaataca
     5341 ggtgtgaccg aacccttgta atttactgtc ctttggcttt tcccaagaat cttttacaaa
     5401 tgattttcca tccaacgcag taattgctgt cgtaatgcga tcttgtagac ctagtaaata
     5461 gtcttttact gcttgcgtat caacactcgt tgccatatct aatccttact taatggctcg
     5521 aaagccaata tcgttacggt attgcatgcc gtcaaaatga atttgtttga tcgcagaata
     5581 cgctttttgt tgggctgcct taacggtatc ggccaggcct acgacgcaaa gtactcgacc
     5641 tccggaggtg attagattgc catcctgtaa tttggtaccc gcatgaaaaa caatttggtc
     5701 atctgtggga ttgggaatac cctgaatgac atcaccagtt ctcggcgtat ctggatactg
     5761 gtgggccgcc atcacaaccc ctagtgctat ccgacgatcc catttcagtt caacctcatc
     5821 gagctttccg tcaatcgctt tatcaagagt atctaccaag tcactctcga gacgtgccat
     5881 gatgggttgg gtttctggat ctcccatgcg acagttgaac tccaaggtct taatctggcc
     5941 atctgggtta atcatcagac cagcatataa aaatccggta tagggaatgc catcggcttt
     6001 catgccaagc acagtcggca agattacctc gcgcattgcg cgtgcatgta tctcaggggt
     6061 caccacgggc gcgggcgagt atgcccccat gccacccgta ttaggacctt cgtcactatc
     6121 acgcaaacgt ttgtgatctt gactagtggc aagtggcacc acatttttac catcgacaag
     6181 aacaataaag ctggcttcct caccaagtaa gaactcttca attacaacgc gcgcaccagc
     6241 attacctaat ttattatctg agagcatcat gtcgacagct gtatgcgcct ctggcaagct
     6301 catggctacc acgacccctt taccggcagc caagccatcc gccttaatca caatgggagc
     6361 gcctttttgc tcaatgtagg cgtgagcttc gattgcattg gaaaaggttt gatagtctgc
     6421 cgttggaata ccatgccgtt tcatgaaagc ttttgagaaa tctttggagg actccaattg
     6481 ggccgctaat tgcgtgggtc caaaaatacg gaggccgtga ttacgaaata catccacaat
     6541 tccagcggca aggggagcct ccgggcccac aacagtcaac gcaatttgtt cacgttttgc
     6601 aaaatctgcc agatcctgta tatcggtgat ggggaggttc tcaatccctt cctgtttttg
     6661 gtttgcagta ccgccattgc caggtgcgac aaatactttt tgaacacggg gggagcgagc
     6721 cattttccag gcaagtgcgt gctctcgacc gccagaaccg attaacaaga ttttcataag
     6781 ttgatcttct atttaaatat taagtattag catttgtata aaccgattgc acatcatcga
     6841 ggttttcaag agcatctaag agtttttgca tcgcctcact ttgatcggta tcaagatcaa
     6901 catccgtctc gggacgcatt gagacctcag caagttcggg ctttagccca gcgctttcga
     6961 gtatttcgcg aatcttagta aattcaaagg gtgcagttgt cacttccaaa gagccatcgg
     7021 catgcgtaat aacatcgtct gctccagcat cgagtgccag ctctaataac agttcttcat
     7081 tggtacccgg agcaaatagg agttggccac agtgcttaaa taaaaaagct accgatcctt
     7141 cagcccccat attgccaccg tgtttagaaa atgcatgccg tacttcggca accgtacggg
     7201 ttcggttatc ggtcatacag tccacgatca ccgctgctcc attgatgcca taaccttcat
     7261 aacggatctc ttcataatta accccgtcta aggttccagt accgcgagag attgcccttt
     7321 gcacattgtc attaggcatg ttggcgtcct tggccttatc aaccgcaagg cgcaaacgtg
     7381 ggtttgcatt tagatctccg cctccgagac gggcagccac ggtaatctcc ttaatgagct
     7441 tggtccatac cttgccacgt ttttcatcct gacgaccttt gcggtgctgt atattggccc
     7501 attttgaatg acccgccatg gtggcaatcc ttatctgata ttgagttaat ttctacttag
     7561 aatcctaatt ttaggcaaaa tagcggtctg acttggaaat accagttaaa tcgtctacag
     7621 catttgattt aaaacaaaaa caattgggag taaatagcat gtctgtaatc actactaaag
     7681 caatccgaat tgatgaggtt ggaccccccg aggtcatgaa gtacgtggat gtcaccttag
     7741 gtgatcccgg ccctggtgag gtcttggttc agcaaaaagc ctgtggcttg aactacatcg
     7801 atatctactt ccgctctggt tactatccac agccattgcc tggcgggatt gggatggagg
     7861 cctccggaat cgttgagaaa gtcggcgatg gcgtacagca tgtaaaggcg ggcgatcgtg
     7921 tggcgtacgc tggaagacca accggtgcct acgcgcaggc gcgcatcatg cccgctgaca
     7981 ttgttgtcaa acttcctgag gcgatttcat ttgagaccgg tgccggcatg atgttacaag
     8041 gccttaccgt tcaatatctc cttaacgata cctataaggt tcaaaagggc gacacagttt
     8101 tattccatgc gattgccggt ggagttggtt tgattgccct gcaatggctc aaagcgattg
     8161 gtgctaacgt gattggcacc gtgggtagcg aagagaaggc agcgcttgcc aaatcatttg
     8221 gttgtgacca tgtcattctg taccgcgagg aagattttgt aaaacgtgtt aaagagatta
     8281 ccaatggcaa gggagtgccc gttgcatatg atgcggtggg taaagatacc tttatgaaga
     8341 ccttggattg tgtttcgcca cggggtatgg ctgttacgtt tggtaatgcc tcgggttcta
     8401 tcccgcctct tgatctcagt cttttgtcga gcaagggatc cttaaaggta actcggccaa
     8461 cacttatgac ctatgcgctc aacagaagtt tattagagcc gatgtcagtg gatctgtttg
     8521 atcgcgttat gaaaggacaa atcaagattg agattaagca aaagtatgct ctagcggatg
     8581 cagttcaagc gcatcgtgat cttgaagggc gaaagactac agggacaacc atccttattc
     8641 cttaaaaggc tgtagagctt gctcagaagt gtttttgggt aaagagggat gagattggtt
     8701 aaaatcaatc cctctgttgt tcttgccatt catttgcagg aacgtttaat tgcctcggtg
     8761 atggaattgg tagacgtgcc ggactcaaaa tccggtgccg caaggcgtgt cggttcgagt
     8821 ccgacccgag gcaccaatca aatttgatag tcgctggatt cgcttgccat tgcctgctcc
     8881 acaattttgc gagtcaaggt tggcgagaag agttcaatga aggtgaagat aaaagagcgc
     8941 aggtaagcgc cttgtttaat accgatgtga gtgacattgg tcccaaaaat atgccccacc
     9001 ggaattgatt tcagtagacg atctcgttca ggatctagtg ccaccccagc cacaatacca
     9061 acccccatac cattttcaac ataagtcttg atcacatcgg catcaattgc ttctaagatg
     9121 atgtcgggag tgaggttgcg ttgcccaaaa gctgcatcaa ttttgctgcg tcctgaaaat
     9181 gctttggcat aggtaatgat ggggtacttt gccaagatct ccagggtaat ggattcatga
     9241 ttgaggagtg ggtgaccaga aggcacgact actgcgtgct gccactgata actcggtagt
     9301 gcaagcacgc cagaggtatt agcaagaccc tcggtagcaa ttgctagatc ggcgcgatcc
     9361 tcaagcaaca tttgggctat ttgactagga ttgccttgct gaatgctcac gcggaccttt
     9421 ggaaagcgtt tcgtgaactc acttaagact ttgggtagag cgtagcgggc ctgcgtatgg
     9481 gtggtagcaa tcacaaagct accttgatct tgatttgcaa aatctttacc gactcgctta
     9541 agtgtttcag tttcttctaa gatgcgttca atcgaaagca agatgcgctt accgggttct
     9601 gtaagagcac gtatacgttt accatgacgt cgaaagatct caacccctag ttcatcctct
     9661 aactcgatga tcgccttgga tacaccgggt tgagaggtaa agagcgcctt cgctgctgag
     9721 gtcaggttaa agttctgacg cacgacctcg cgaacaaagc ggagttgatg aaggttcata
     9781 aatcaggaca ggttatgcgc gcgccaccca cttgagggcg atgattgcta agactaagta
     9841 aataagaggt ggaaggatag cagtcaggag cgcaggtagt gatcccaata aaccaatgct
     9901 cgaaaacaat gtattaaaaa gttgaaagct catccccagc atgatgccac cgaagacttt
     9961 gatacccacg ctacctgcgc ggaccttcat aaaggcaaat ggaagagcca atgccagcat
    10021 cacaaaaata ataaatggat aaataacttt tttccaaaag gcaattgcat gcctttgcat
    10081 atcttgctta ttatcttgta ggtgaagaat aaaacgcccc aggctgacaa tcgacatttt
    10141 ttcagggcta attaatagaa cattcagaat ttgaggagtc acctgcgact ccagacttag
    10201 cttcggaagt attttggttt gtgctgtgta gaccgcatcc aaggggtcac tactgcgctt
    10261 ttcggtgaaa cgagtttctg agacatcatt gagttcccag atgccccgat tatcaaagcg
    10321 gccagaggct gcgctccgta tcgatagaag acgatagttt ggatcaaatt catacattcg
    10381 gatttgtcgg atttggtcat tctgatcaat agcgcccaca ttgacataac gaacgcctgc
    10441 tcgaattggg ccggtaccat cactatctct cagttggtct tttacccaaa ccccagatcg
    10501 aaactgagaa ctaaaggtgg agcccaatgc cttcatccgg actcgctcag atagagattc
    10561 agcatagggt ccgagaactt cactaatgag tagggtgaac actacgattg gaagcgaaat
    10621 ttttccgagg gtaagtagac tgcgtttgat atccaaaccc gcaacccgaa agatggtgaa
    10681 ctcggattgg ctagccatca tggcaaaaac ataaatactg ccaatgagac ctgcgattgg
    10741 aataatttca accatcctgc taggggcttt taataaaaca tgaagcagtg ctagagggag
    10801 agtgtatttt gcgttgacag agccaagctc acttagaaca tcgaagaata gaaagagagc
    10861 gacaagagca aaaaggataa aaccaaagct ggcataaatc tgctttgcaa agtaccgttc
    10921 gtagattttg ggaaacagcc aactcatttt gattgaaata tccagggtag ttggcgttgc
    10981 caccatttca gggatgggtt aacgcggtta cgaattaata cataagccag taaaaaggca
    11041 atcaagtgga ttggccaaag cccaacaaaa aattcaaggc gaccctgcga gacgtaattt
    11101 tgagtgagat ttaataaatt actgtagatc aaatacacca acacggcata gaacatggcg
    11161 gtgtaattac caagacgtgg gttgacgtat gccaagggaa tagcaattaa tactaagcct
    11221 aaggccatga atggcaagcc aattcgccat aagagctccc caagattagc acgcttgata
    11281 ttggggtcaa cttcgttaat gagctcaaga atatttttct ctcggtctct cggtggcgga
    11341 gcggcaactt ctttgccacg tattttggtt tcgtactctt caaactctaa gattctgaaa
    11401 ttagcttgcg taggctgacc ttcatagcgc ctccccttca gtaaaacaat gctcttatca
    11461 ccatttggag cattctcaat gaagccagtg gctgctactg ccacactcaa tttctgattg
    11521 cgggtatcgg cgacaaaaat attcttaacc tcacccttgt cgacatctag gctttcaata
    11581 aaaaatactc tctccgcctt ggctgattct ttaaactgac ccgcggttac cattgatacg
    11641 tcatcgcgtt gttgaaagcg ctgactaata atgctggatt cacgattggc ccacggccag
    11701 actacggctg ccagaatagc aatgatgata attagtggaa tcgcaaaacg taagatcggg
    11761 ccaattaaat ttgtgatgct taatccactt gtgaaccaaa caatcatttc cgagtcttta
    11821 taccaacgaa ccaaaacaat taagactgca ataaaaaggg aaacggttag cagaattgca
    11881 aagtagccta gagttgccag ggcaattaga acgagggcat cctcagggtt cactaccccg
    11941 ttagcggcaa aacctagaat acgaatcacc aaggtggtga tcatgatcgt aacgagtacc
    12001 aaaaatacgg cacccgtggt catgctaagt tcgcggcgga gggcttgttc aaaaatcatg
    12061 tcttaaatag tgtatttggg cagggtcaat tcgctcggtg gataatgtaa gaaataacct
    12121 ttatatagac agatcatgac aattcaattt agcacgaaag ttctctccga gatcgatttt
    12181 aagagcccaa aggccgctac tgcaatttgt ggcttatctg gcgattgttt gatccttggt
    12241 taccgtcagg gcgagttgaa ccaattacag gctctcgatg atttattggg tggcgcaatt
    12301 gggcaagctc gtagcctggg ggatctcgac ggtaaggccg gagcctgcac cttgttgcgt
    12361 tccacccatt catgggcatt aagtaaggtg aggctaaagc gtgttttatt agttggtcta
    12421 ggtgataaag aatccttggc tgattttgca aaggttgcac gttcggccat gaagcacatt
    12481 agtgctagtc caatagagtc ggtcttatgg catgtgggca gctttacggg taaacataaa
    12541 acgaatcaat cgccaacagc tattcaggag cgcattcgtc ttctcgcgca aattgtgggt
    12601 gatcaggcat atcactttgg cgttcgtcaa gcgcaattta agactaagcc tgtagcaaaa
    12661 tcagacccac tgaaattagt cacgctgctt acaaatacaa agcaagctgc cttagtgaag
    12721 gcatctgttc atgagggggc ggctttggtc gagggtatga acttgaccaa agatttgggt
    12781 aatctccctc ctaatatttg tacccccaca ttcttagcgc ataccgccca aaatttatcc
    12841 aagaagtcta gtcttcgcgt tcaagtgctt ggcgagaaac aaattgaagc tctggggatg
    12901 gggtctttct tagctgtcgc aaagggttcg gatacgccgc cgcaattcat catcatgcgt
    12961 catgatggcg gcagagccga tgaagatccg attgtgattg ttggtaaagg aattaccttc
    13021 gataccggtg gtatctcctt aaaacctggc gaggccatgg atgaaatgaa atacgatatg
    13081 tgcggggctg catcggtgtt tggcaccatg catgctgtat cacttcttaa tttaaagaag
    13141 aatgtgattg gcgtggtccc cacctgcgag aatatgccat cgggtcgagc aacccgtcca
    13201 ggtgacatcg tgaagagcat gtcgggtcag accatcgagg tattaaatac tgatgccgag
    13261 ggacgcttaa ttttgtgtga cgccttaact tatgttgaac gctttaagcc aaaggcagtg
    13321 attgatgtgg ctaccctaac aggtgcttgc attattgcct tggggcacgt gcacagcgga
    13381 ctgttctcgg atgatgagtc cttggtcaag gatttaagca aagccggtca tcaatcattg
    13441 gataccgtct ggcgcctacc tttagatgct gcttatcacg agcaactcaa gtctaatttt
    13501 gcagatatgg ccaatattgg cggccgtcct gccggtagcg taaccgctgc atgctttttg
    13561 tcacgctttg cagagaaatt tagatgggcg catttagata ttgctggaac agcatggaag
    13621 agtggcgtag caaaaggagc aaccggtaga cccgtacctt tattgctgaa cttcttgctt
    13681 gatcaataga gttagcaggc ttagtcacta atggcacgca ttgattttca tagcaatgtg
    13741 gcagacaaac ttctatattg ttgccgcttg agccgaaaaa tattagccag cgctgactct
    13801 acgggacagc atcgcaccat tgtgattgtt ggcaacctta aagaactcca agatttagat
    13861 gccttaatgt ggagttttag caagactgac ttcttgccac atgcatggct tgaccatgat
    13921 gctgcacaag agacccccat tgtttttgta agcgatgtta atcaactcaa agatagttcg
    13981 attccgcatt gcgatgtatt aatttatctc aagaatgaag caccgccaca actcgatact
    14041 ctcttagctc gttttccaag atgggttgag gtggtcacaa cccaggagaa tgagcgttta
    14101 gctggccgtg agcgctacaa gtcttatcgc gagatcggtc atgagttgca tcactttgat
    14161 caagcaaagg ggagctaaga catgctaatg cacccgcaat ttgatcctgt tgctatacag
    14221 cttggcccgc ttgcaatcca ttggtatggc ttgatgtatt tgttagcgtt tgctcaattt
    14281 ttgatattgg gtcgaatgcg agttcagcaa gagccattcc tcagtttgaa gtggagcttt
    14341 aaggatgtcg aggatatttt gttttggggt gtagtaggcg tcatcttggg cggacgtctt
    14401 ggttacgtct tgttttatat gccagatttt tatttaagca atccccttaa catcttcaag
    14461 gtgtgggagg gcggcatgtc atttcatggt ggcttactag gagtcttggt tgctttgtta
    14521 tggtttgcta aatctcgcaa gctgtcttat tttgtagtga ccgattttgt tgcacctttg
    14581 gttcccttgg gtttagcgtt cggacgtttg ggtaacttta ttaatggaga gttgtggggt
    14641 cggccgagtg attggccgtg ggcaatggtg ttcccagcgg tcgatcaggt agcgcgtcac
    14701 ccctcacaac tctatcaatt tgctggtgag gggttgctgn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnagg gagcccgatg cattcttggg ccttttggga ttgggtctat cgatggggca
    14881 gtggctatcg ctaccgatga ttggattcgg tatttatcta atcgttcgga cctctcggtc
    14941 gtaaaaatcg gacatttttc ctaaaaccta acacgaagtt tggaaagctc gttatcctgt
    15001 aacgatgcta gaaaaaattg ctcagactcg tcatttatcg cgtgcgattg gtgcggttaa
    15061 tcgcttagtt tctgagcgag gggagtctaa tgcagttagt atggctgccg atgtaatttt
    15121 taattaccgt aaattaaatg ctgaacagcg ctccaagttt tttatggcct tagcggagca
    15181 atttaatatt aatgtggaag ccctgacaaa agcgacacaa agtttttctg cagacccaag
    15241 tgcccgtaac tacattcgcc ttcaaaagat ttcggagtcg ccaagacaag agcttttgcg
    15301 ccgcttaaat cgcgcccctg ggggaactgc cgcggttgtt gaaatgcgtc gtgatctact
    15361 aagcctgctc cacaagaaac ctgaattggc tggcttggac tacgacatgc ggcatttgct
    15421 gtcatcctgg tttaatccgg gctttctaaa aatgcaccga gttgactgga aatctcctgc
    15481 agaggtcttg gaaaagatca ttgcacatga ggcagtgcat gcgattgatg gttgggatga
    15541 tttacgtcgt cgccttcagc cagaccgtcg gtgttttgcc ttctttcatc cgcagctgcc
    15601 tgatgagcct ctcatctttg tggaggttgc gttattacct gaaatcccag tagcaatcat
    15661 gccattggtt gataaaaaat cagcgcctgt ggaacaaacg aatcaatata aggtagctgc
    15721 cttctattcc attagcaact gcgaatcagg cttgcgtggt gtctcaatgg gcaatttttt
    15781 gattaaacga gttgccgagc agttgcatgc tgagttcccc ggactcaaaa cctttgtcac
    15841 tctatccccg atcccgggtt tgatggaatg gattactgct ggggcgcatt tgggagaggg
    15901 gccaagtgcc gataagatta agcctgcgat tcgcaaggcg cgtgatgagg ctcttgagct
    15961 tcttaaatta agcggcacat cgtggccaga gaaattatca aaagcatggc acccagatgc
    16021 atgcagcaag aaagagaaag aggcatttga gtgcttaaca gccatttatt tagcttgtgt
    16081 aacgccgaat cgggatggaa atccagtggc gaagtttcat ttaggcaatg gcgccaaatt
    16141 acatcaaatt aactgggccg gggatttatc caaaaatggc ttacgccagt ctgcaggcct
    16201 gatggtgaac tatttatatg accttgctag cgttgaggaa aatcatgagc agtttgtgca
    16261 tggtgagatt atttattcta ggtctgttgg taggctcatg aacccttaag ggcaagtcat
    16321 ctataagact tttaggggtt tagccctatt tttagcagtt cgatttagaa taatgttcgc
    16381 atttaattta gaccttataa gaattgaaga ggagacaaac aaaatggttt ttagtccaaa
    16441 gacaagaatt gctggcgtat tagttggctt attagctgta ctgggtattt ctagtactgc
    16501 catggctcaa gagtggcccg ttaaaacaat aacgtttttg aacccattcc cagccggtgg
    16561 tggtaccgat gcatttgcac gccccatagc agctcagttg accgagcagt tgggcaagca
    16621 ggtcattatt gataatcgtg gtggtgctgg tggcactgtc ggtgcatcgg tcgctgcgaa
    16681 agcagctcct gatggctata cctggtttat tggtgcatcc catcacacca ttgctccctc
    16741 catgtataaa acgctcgact acgatattca gaagagtttt ataccagttg ctcttttagc
    16801 cagcgtaccg caagtattgg ttgttaaccc aggtcgagtc agcgcccgaa atcttaaaga
    16861 ttttatagcg ctaatgaaga agaatcctgg taaatacaac tttgcaagtg ctggtagtgg
    16921 cacagtgcat caccttgctg gtgagttgtt caagatccaa accggtacct tcattgttca
    16981 tatcccatac cgtggcgccg gtcctgcaat gagtgattta ttagcaggtc aggttgatct
    17041 tgagtttgat ggtcttggca cttcagcccc acaaattaat gccggtaagt tggttgctat
    17101 tgcagttgct gctgataaac gtaatcctgc aattccgaat gtgccaacat ttgcagaagc
    17161 aggtttgcca gattacaaag tatcgacttg gtatgccatg tttgctccag caggaacacc
    17221 taaaccaatc atcgacaaga tgaccgcaga agttcagaag gctttgaata cacccaagct
    17281 aaaagcaatt tggactagca acggtgcgga gacccctaac ttatatggcc cagcatttgg
    17341 tcagttggtt gcggctgatg tcaatcgttg ggcagaggta gttaaaaaat caggcgctaa
    17401 gctcgattaa tgatttgttt ttgtccgtga gggttttgta gtgaacttat ttgcattatt
    17461 agaaaaaggt tttcccaaga atcgcgaaga ttgtgcacta gaaaccccgg acggcttgta
    17521 ttactcgtgg cgtgatatcg atcgcgccac agccatgatg gcgaacctcc tcaagagcct
    17581 taagttacct ttgggctctc gagtggcggt tcaggttgaa aaatctccag aagccttatt
    17641 tctttatcta gccacattac gggctggcta tgtttatttg cccctgaaca ctgcttatca
    17701 agagtctgag attcagtact ttattgaaaa tgccgagcct gcggtggttg tgtgcagcac
    17761 caaaaacttt tcttggattt ccaaagtcgc atttaaggct ggcactaaga acgtatttac
    17821 cttagacgat aaccgcagtg gttcgctgct tgagcgagca tcgcattgtg cagatcaatt
    17881 taagac
//
LOCUS       DDPQ01000037            2611 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_26235,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000037 DDPQ01000000
VERSION     DDPQ01000037.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_26235"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT          693 a          618 c          628 g          672 t
ORIGIN      
        1 aaagtgcagc acagcgtttg cagcaaaatc ttcacggata tagcgataaa acgctgaaaa
       61 tgcatgcgta ggagcaaagc ccttttcaaa caagagacgc atgggatcgc cctcaaaacc
      121 aaaaccaggt tgtacagtca ccaagacatt gccgtattgc ttacccaaaa taaatatgcc
      181 tgaaccattg ctccattgct taccaggagc aggcccccat tgcgcttcaa tttcttttaa
      241 ccaaggatca cgccgaacgt gatcgtccgc agagatcaca gtgtggacat ttgcttcagc
      301 tccatactgc tgggcatttc cctcaataat ctctttacgt aatgcttcag cagtttcagg
      361 aacgcttacg gtatagccgt ctgctttgag gcctaacatt gttttatgta aggattcaaa
      421 tacattgagg taagcggcgg tgccagtgtg cccagcattg ggtgggaaat taaagattac
      481 taaaccaatt tttcggttag ctttgtcaga cttacgtaaa gccaccaact tagcggtgcg
      541 tgcggccagc attgcagtcc gctctacgca ggtaaacata tcttgtggat tatgttcttc
      601 ggtaaaggtg cagcgttgat ggcaaccagt acaggtcgtt ccagcggcgc ctggtcggcc
      661 accgtagacc attggctgcg ttgcgccatc cagttcagga atggctacca tgatggtgct
      721 ctcaaccggc attaagccac gatctgatcc accccactgc gttagcgtct gaaattccac
      781 tgggtgggca gcaatgtacg gcacatccag tttagcgagt acctcttcgg ctgctttcgc
      841 gtcgttgtat gcggggccac ctaccaggga gaagccggtt agagagatca ccgcatccac
      901 ggtgacctga ttatttttca tgaagtaaga atcaatggcg gggcgagagt ctaagcctgc
      961 cgcaaaggcc ggtaccactt gcaagccatg tgcttcaagc gctgcaatca ctggatcgta
     1021 atgaccacta ttgcctgata ctaagtaaga gcggagtaat agtaagccga cacgaccacg
     1081 actctttgca tccggaataa ctttgggtag atcggtgagg ttttcactca agcgattttt
     1141 catacggggg tgatagacac ctagatcggg atactccacc ggatcagtag gttttgctaa
     1201 gccacgtagt tgttcacgag gaccggcagc ataccgatta attaaataac taaagaggtt
     1261 gaagatattt tcttcagagc cgcctagcca atactgtaag gttaaaaaat agacacgcac
     1321 atcctgtgca gtgccaggga tccaacgcaa gatttgcgga atcagccgca ataccttcat
     1381 ctgcttagcg ccagaactag tttgtttctc accctctgtt ttagccttag gacgtaattt
     1441 cttaaggatt gccatcggac cacttgctgg cttactcatg tcgagtttgc caattttggt
     1501 cagtttgact acatcgccgg cggacatcgc acaaaccatc gcatcacaat catcccgctt
     1561 tgcttttagt gcctcaaaga caggcaggta gtgatcctcc ataaagagca tggtggcaag
     1621 cacaatatcc ccggctgcta tatcggtaag cgccttctgg gtaagctccg gattgccagc
     1681 aaactcactg gccgcatgaa tttgcaggga taaacctgga atagagcgtt gcagctgaaa
     1741 ttgtgcccgg cgcgcggcac tatttaaatg cgtatccatc gttaccaata ccaaacggat
     1801 tgggattgct gtttgtttgg atttctgggt tacagccatg gtgtttagct ccaaagggga
     1861 ctcccctttt tatattggtt tcatatagat gtgtcaactt ttattgacac ttatggctgt
     1921 aattgccatt aaattaaggg ttaaccctta aaagtaatca ttcatggcga tcttttctca
     1981 cacgatctat aaatagaggt gaaaaaaaag atgtactaaa taggcctgat tttcagtaga
     2041 ggtttggcta gcccctaaga cgaaatacct taagaaaatc ggagatagta tggttctttg
     2101 gcgatatgcc agtctcatca aggaaaagtt attttccagg gatgagcaac agcagaacat
     2161 caaccctagc cgggctgaat gggacgagtg tgttcatgaa tcagccaatg acagcaatcc
     2221 agaccgtatc tcccaattag cagttgatct tggtagtcga ccgctgcgca ccaatgcagg
     2281 acctaatgaa catttggatt ggctcatgca aactattgag gctaatgtca taccgcgatt
     2341 attgatggcc cattccggac atgcacaagc cagcaccgca ccggtgatga acattcgttt
     2401 ggatgatcaa acgcgtgttg aagaactgtc tcggttggtc ctgcaggatg atgccagtcg
     2461 ggcagctcaa tttgtacaag agctctacga acagggagta cccctcgatg agatctatat
     2521 gcgattgttg gctcccgtag ctcgacgtct tggaacaatg tgggaagaag atcgagccag
     2581 atttacgcaa gtcacaactg gtatgtggcg g
//
LOCUS       DDPQ01000038            9630 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_6482,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000038 DDPQ01000000
VERSION     DDPQ01000038.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_6482"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    178..218
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    392..434
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3338..3409
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5177..5288
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8215..8285
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8539..8628
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2369 a         2236 c         2094 g         2502 t
ORIGIN      
        1 ccgggtggag tccacccttt attaataggt aaacagtggc gacaaccaaa aaaactagca
       61 aagcgagcgt aagcggggaa cacgccaagg ctctcatcat tgccgagaag ccctcggttg
      121 caaacgacat tgcaaaagcc ttgggtggct ttacaaaaca tgatgagtat tttgagannn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ttcatcggca gtgggacatc
      241 ttcttgaaat tgccgctccc gatgaatatg aagtgaaacg cggcaagtgg tcatttgcca
      301 acttgccggt aataccgcct cactttgatc tgcgcccaat tgttaaaacg gagtctcgcc
      361 ttaaggtctt acaaaaactg atcaaacgaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnaatgca tgtgacgcag gtcgtgaggg cgagctcatc tttcgcctaa
      481 ttgcccaaca cgccaaggcg ccgcaggcag ttgaacgtct ttggcttcag tcgatgaccc
      541 ctgcgtcgat tcgggagggc ttttctaagc tgcgctccga cagtgacctg caaccgcttg
      601 ctgatgcagc acgctgccgc tctgaggccg actggctagt tggcattaat ggcacgcgag
      661 caatgactgc ttttaatagt aagggcggtg gctttttctt aacgaccgtc gggcgcgtac
      721 aaacgccaac cctatcaatt gttgtgcagc gtgaagaaca aattcgcaaa tttattccca
      781 aggattactg ggaggtgcgc ggtgagttta ttgcggcggc tggaatctat gagggacgat
      841 ggtttagtcc tgattttaaa aaagatcctg ccgaaccaga tgcaagagaa aatcgattat
      901 ggagtgaggc tgctgctaaa agcattgtgg ccgcctgtcg aaataaaaaa ggatctgtca
      961 gcgaagagtc aaagccggcc actcaacttg ctccacaatt atttgacctc accagcttac
     1021 aacgtgaggc aaatgcgcgc tttggcttct cagctaaaaa taccttaggc ctcgcccaag
     1081 ccctgtatga acggcataag gttttaacct atccacgaac agatgctaaa gcgctgccag
     1141 aagattattt agacaccgtc catacaacca ttgaaaacct gggtgaacat tcacccgaat
     1201 ataaatcctt tgcaagcacc attctcaaag gggatggtaa gaaatcgtgg attaagccta
     1261 ataaacggat ttttgataac agcaaaattt cagatcactt tgcgatcatt ccaacccttg
     1321 aggctcctaa aacactcagc gagcctgagc agaagctata tgatcttgtt gtgcgaagat
     1381 tcttggcggt cttttatccc gctgctgagt atcgtgtaac aacgcgcatc acagaggtgt
     1441 ctgggcatca ctttaaaacc gaggggcgcg tattggtgga acctgggtgg ttaagtgttt
     1501 atggcaaagc gagccaagct gatgatgagt taatccccgt ccaaaaaaat gagtcggttc
     1561 aaagcgaatc aattgaactt gttgctctta agactaaacc gcctgcacgg tataccgagg
     1621 cgacacttct ctctgcaatg gagagtgctg gtaagtgggt ggatgatgac gacatgcgcg
     1681 aagcgatggc agaaaaaggt ctggggacac ccgctacacg tgcagcgatt atcgagggtc
     1741 tactcgcaga aaaatatatg gtgcgtgaag cgcgcgagct tattccaaca gcaaaagcat
     1801 ttcagttaat gaccttattg cgtggcttag acgtatctga gcttactcag cctgatctca
     1861 ccggaacttg ggagcacaaa ctttctctaa tcgagcaagg caagttagat cgcaatacct
     1921 tcatgcaaga aattgcgcaa atgacgcaac gaatcgtcaa acgtgccaaa gaatttaata
     1981 gcgacactat cccaggtgac tacgtcacac ttaaaactcc ttgcccgcat tgcggtaagg
     2041 ctgttaaaga aaactatcgg cgctttgctt gcgagtcatg tggattcacg atcagcaaaa
     2101 cgcctggtgg cagagccttt gaatacgacg aagcagaaac gcttctgaaa gataaaacta
     2161 ttggccccct tcaaggattt cgtagcaaga tggggcgtcc ttttgctgcg attattaaac
     2221 tctctgaaat acccaaggac gatgccgact atcccaatgc gggctttaag cttgagtttg
     2281 attttgggaa cagccaagat gacgatactg aagtcatcga cttcacaggc agggaggcgc
     2341 ttggtgcatg ccctaaatgc tccggggcaa tttatgagga tggtatgcgc tatgtgtgcg
     2401 aacacaatac tggcccaacc aaaacatgtg actttaaaac gggcaaagta gttcttcaac
     2461 aagaaatttc tgcggagcaa gttaaaaagc tgttgataga gggtaaaact gatctattaa
     2521 ccaactttaa gtctaaccgg acggggcgcg gctttaaggc ttatcttgct ttggacgctg
     2581 gaggaaaaat tggttttgaa tttgaagcta aggcgcccgc aaaaaaacgt gttggcgaca
     2641 aagacgagag caagtccgac cccaagccca aacgggccag tcgatctaaa gcaaaagcgg
     2701 aagaatgaga tagcgctggt attacgtagg aggcaagagt ggcccatagt agaccccttg
     2761 atcccagcgc actcaccaca taaacattct ctaactgggg cagagcacct ataagcggta
     2821 gtcggtcttt ggtaacgcat cgtagaccta caaatcggtc gattggccga agctcttcta
     2881 agtctaattc agcaggtgcc aacccccttg ctaactcaag gttgtattgg tcgctgctct
     2941 ctctgggatc taagccacca tcgtgttcgt cataagtcga gcccaccaac cagtgatagc
     3001 gctgatactt tttgtcatag cgcggtggca agcagtaccc ctcccctgac acaataattt
     3061 ttggtagttg gtgcaccagc tcagaatctt ttgccaaggc aaaaatagtc agctggccgc
     3121 gtaccggttt taaaaaaaga tcgatatcaa attgcttggc caaggtttgc actccgaagg
     3181 cgtttgctaa tattagagtg cgcgcccgct gcaatgtgtt tcccccttga tcccgtgcga
     3241 tccaaatacc attttcattt tcaatgcgcg ctacggtctt tgaccagatc gtgcaaatgg
     3301 ccgcactctg ctccacaagg tcttcacata gctgcccnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gggccgatgc
     3421 ctaaacgctg taatttgtct gcgagtacca catgatcaaa tggtgtgttt gctttgcttt
     3481 gcaaaaaaac gccgcgcgtc tcccagcgat cttgccaatc atccgcaacc agccgatagg
     3541 cccctaatga gatcgtgtgt agtttgctcg cagtgcggcc aatagcgggg tgagtaatcg
     3601 cacccgcatg tgccgaagtg acttttccgg gaccattttg tgcatcgatg atgcaaactt
     3661 ctatgcctag ctgacaatac tgtttggcca atgcggcgcc agcaatgccg gcgcccacaa
     3721 tcaaaacatc aacatggtct ttaggcaaag aggcttaaga tccaaagcgc cgctgaaatt
     3781 tggcccgagt ttcaattagc tcttttggta ggcgatgcgc cagcttatca aaaagctctt
     3841 ggtgcaaatc gagctctgcc ttccagtcgt cggcttgcac cgcaattgat ttattaaact
     3901 gctccgcaga taggcttaag ccattccagt ttaggtcggc gtggcccggc gagtgtccta
     3961 ccggtgtctc ttgtgcccct gcagtatttt ctgctcgccc taaaatccat gacaagaccc
     4021 gcatgttctc gccaaagcct ggccaaataa atttaccgtt ctcgtctttg cggaaccagt
     4081 tcaccaaata aattttgggt aaattcgcac cctctgactc taacttcttg ccgatattta
     4141 accaatgttg gaaatagtcg ctcatgttat atcccgcaaa tgcgatcatg gcgaacggat
     4201 cgcgccgaac cactccaacc ttacccgtaa ttgcagccgt ggtttctgaa cccatggttg
     4261 cagccatata aacaccctcc acccagttgc gcgcctcggt gaccaatggc acggtggtcg
     4321 agcgtcggcc gccaaaaata aaggcgtcaa gtggcacgcc ttccggattg tcccaattag
     4381 gatcaaccgc tggattgtta gttgcggcca tcgtaaagcg cgcattcggg tgtgcggcca
     4441 agcgacccgc cttgccgtct gctggggtcc agtccttacc ttgccaatca atcaagtgac
     4501 ttggtggggt ctccgtcaga ccctcccacc agacatcgcc atcatcagtc agagctacat
     4561 tcgtaaagat aacgtcttga tttaaagagt cgacacagtt ggcattggtg gcacgattgg
     4621 tacctggagc cacaccaaaa tatccagact ctgggttgat cgcatacagc cgtgtttgcc
     4681 ctgtaacagg atctttgcgt ggcttaatcc aagcaatgtc atcaccgacc gtggtgactt
     4741 tccagccctc aaaccccttg ggggggatca tcatcgaaaa gttagtttta ccgcatgcgg
     4801 atgggaaggc cgcggccaca tgatattttt taccggctgg tgaggtaact cccaggataa
     4861 gcatgtgctc cgccaaccaa ccctgttcac gccccatggt agaagcaatc cgcagagcaa
     4921 agcatttttt accaagaagc gcattaccgc catagccaga accaaaggac caaatctctc
     4981 gggtctctgg gtagtgcacg atgtacttgc ttggattgtt tggccacaca acatcctttt
     5041 cgccatgagc caaaggtttg ccaaccgagt gcacacagtg aacaaactcg ccgtgctcgc
     5101 ctaatagctc tattacgccc ttacccattc gggtcatgat gcgcatatta attgccacgt
     5161 atgggctatc cgacaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnncc cgcatcatct ttgttttgcg agcaaataaa tgtgcggtct tctactcgcg
     5341 caacatccat tggatcagat attgctaaat aagaattttt gcgttttgct ggatttaatt
     5401 tttttaggct tcccgctttc acaagcaact cacacagcgc gtcatattca gtaatcgagc
     5461 catcgcacca atgaacttgc tcgggctcag tgagcgtagc gatttcttga acccactgaa
     5521 ttaatttttg atttttaaca taactcggcg cgccaatttg tactgcgctt ggtatggctg
     5581 gtttgttcat aagaccctca aatgtaaacc cttaattatt aacgcttata ttttttattg
     5641 ttaatctatt tgtatcaatt ttaacatttt tgtaacatta attcggcctc ttatttgccg
     5701 atacaaaact cactaaaaat ttttcccagc aactcatcgg gcagcaactt gcctgtaatt
     5761 tgaccgagtg catcctgtga aagacgcaat tcttcggcaa acaaatcaag tgaatggttg
     5821 ccgtttaaac cgtgggcctg agcctttaat aaagattctt tggcctcagc tattgcactc
     5881 aaatgtcggt cacgcgccgt aaatacaccg gccgctaagt tcgcttgcca gcccgctaaa
     5941 ctcaaaattc gctctttcaa agcatctatc ccttcgcctg tttttgctga aatatgaatg
     6001 gtgttctttt cagatgtgga tatgcctagt tggcgctttt gcgaatcgac taaatctaat
     6061 ttgttaaata cctcaatgat gtgggctttt ttattagcac gtgcttttac tttttcttca
     6121 agcccgccat cttctccgct ctgcgttgtt gaaagatcgc gcataaaaat aatcgtatcc
     6181 gcgtcttcaa tcgcatccca agttcttgca atgccaattt gctcaaccac atctgccgat
     6241 accctgaggc ccgcggtatc gaccaagtga actggaactc cctgcaatgt aatgctctcc
     6301 tcaatccggt ctcgggtggt gcctgccaca ggcgtcacaa ttgcgcgctc ttcgcctaaa
     6361 aggcaattta ataatgagct tttaccaaca ttgggcgatc ccacgagggc aagacgaatc
     6421 ccatcgcgta agatttttcc ttgttgagtt ttttctacaa gatcttgtaa tttttttaga
     6481 atgcttgcta gcctctcatt cgcttttgag ctttctaaaa actcaatttc ttcctccgga
     6541 aaatcgagcg tcgactcaac caaaatacgc agctgtgtga tttcttcgat aagctgatta
     6601 acgctatcag aaaactgtcc ccgcagggag cgtgcagcgc cttttatggc tgcatcactt
     6661 tgagcattaa taagatctgc aatggcctcg gcttgcacta ggtcaatctt gccgttcaga
     6721 tatgccctta gggaaaactc tccggcctcg gcctgtttaa taccccaatc attacccagt
     6781 tcgagcaccc tgttcaatat tgcctgcgac aagtgtgcgc cgccatggca atggagctca
     6841 ataacgtctt ctccagtaaa tgaggccggt cccttaaaga aaatcaacag agcttcatcg
     6901 aggcgatttc cctgtgcatc atttaagaaa aaatgatggg cgtagcgtgt tgtcgggggc
     6961 ttagccaata aggcctctgc taaaccaaca agctgctgcc ccgacacccg aattatgcta
     7021 acgcctccag agcctggcgg cgttgcaatg gctgcgatgg gatgttttct catgcggtcc
     7081 ccatcggagc caactattta ttcgcgcttc gctttgcaga catttggtta atttgccact
     7141 gctgagcaat cgacaaaata ttattaacaa cccagtaaag aacaaggcca gctgggaaga
     7201 aaaaaaacat gaccgagaaa acaattggca tccacatcat gatcttggct tgaagtgggt
     7261 ctggtggtgt tggatttaat ttggtttgca caaacatcga caccgccata atgaccggca
     7321 aaatatagta ggggtcgggt acagacagat ctttgatcca cagaatccaa ggggcattgc
     7381 gcatttccac tgatgataaa agtacccagt acaaagcaat gaagacggga atctgaacca
     7441 caactggcag gcatccgccc agtggattaa ttttttcttg tttatagaga tccatcatgg
     7501 cgcggttaag cttttgtgga tcacccttga attgttcgcg caggtttagg agtcgtggct
     7561 gtacctcctt catacgtgcc atggatttat agcttgcagc ggacaatggg aaaaatatta
     7621 acttgataag cagtgtaaga ataacaatcg cccaacccca gttggcaaca taggaatgaa
     7681 tttgttcaag caaccaaaaa attggttttg caattaccgt taaatagcca tagtccttaa
     7741 ttaaatcaaa gcctggagca atcttctcta atagagtctc ttcttgcggc ccaataaata
     7801 aacgagtctt ttcaacatgg cttgcgccaa cagcgagagg gttgatcggg gtttgtagac
     7861 caatgcgaaa tagattctgt tcgaccttgc ctacatagac atctcgcact gctttgtcac
     7921 ttggcaacca tgcgcttgca aaataatgct ggaccatcgc aatccatcca ggctcacctg
     7981 cggggatttg ttttgggatg tttactttat ttttttcgat atctgaaaaa tctatctttg
     8041 tgaacttttc tttatcggta tatagagcgg gtcctgtaaa ggtgctataa aactcgctgt
     8101 cactaacagc ggtaccatcc cgtactagct cggtgtagag caccagttgg ttggtgcctg
     8161 cttgattact tatttgcgta attcgatgct ctacctctac aacatagcta ccatnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnntgatt tggcagatca ctactaacag ccgatattaa tccagagcgc gcaaaatatt
     8341 ggttttgccc tgtgatttgc aaaaggctta ttggtttttg gtccgcccct aattgcttta
     8401 gtagttttgc actgattacg ctggcgcccg tagcgctgat ttctaattcc agcacttcat
     8461 ttttaatgct gtgtttctct ccggtcgtta agcctacgac gcttagtgat ggtccttgcg
     8521 gtggcttttg tgttgtcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tctttttgct
     8641 ctggtgactt agaaacgttt ggggttaggg gtgttgggcc aaaaagcgcc tgttgaccat
     8701 catatataag ccaattgtta tagagcatca agcccgaaat ggttaataca gcccaaagta
     8761 gtgttcgctt gatatccatt tttaattgct tatcttggtg aatgattgtt gggtgggttc
     8821 tttgacaacg ggatcatagc cgccagatga ccagggattg caacgcaaaa tgcgccaact
     8881 gattttaact aggcttttgg ttgagttgtg tatttttaaa cactcacacg catattcaga
     8941 gcagcttggc acaaacttac attgggaccc aaaaaaaggt gaaagggtaa gctggtaggc
     9001 ccgcaccaaa gaacttagca atttattaac cagccgcatt ggcaagctcc attaatttag
     9061 ctcttagcgt tgtttgttct tgtttgcgaa gcctgccttg ggtttttttt ccaactgaat
     9121 gacgcagctt aataacgcaa tccgaatttg aaccgaagat gcgtttgcgc gccaactctc
     9181 tgcttaggcg ttttagttta tttcggtcaa ccgctcgctt tgctaatttc tttggaatgg
     9241 ccaagccaag ctgtggtcgg cccccttgcg tcaacgcttc aatgtaaaca gcccagcacg
     9301 cattcgcact tggtctggct ctcaaaagct ctgaaattct gttggatctc aacgcattaa
     9361 acggctaggc gtttgcgtcc ttttgatcgt cgggcgttca atacggcgcg accgcccttc
     9421 gttttcattc tgacgcgaaa tccgtgggtt cttttacggc gggttactga gggttgatat
     9481 gttcttttca tgtttaatcc taagaaaacc ctctattctc tccatttgtt ccattagcgt
     9541 caacttaata caagggtttt gcctaatttt tgtgtttttt ctctttaatt tctaataaat
     9601 ctttgttttt taatgggttt gatctctttt
//
LOCUS       DDPQ01000039           12882 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_10403,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000039 DDPQ01000000
VERSION     DDPQ01000039.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12882)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_10403"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1087..1120
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3588..3712
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8986..9108
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9431..9450
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3686 a         3193 c         2654 g         3034 t
ORIGIN      
        1 ctcggtggtc ttgaagttaa tcaaaccccc gtcttaacca atacctgcta tagctttgtt
       61 agtgctgatg aggttgtgca cgttgcaagt gttcatcaat acgacgccaa agacaaaacc
      121 tttaaaacag ttccgggatc aggcggcctc tctgcggaag cgagtaagct tgaaggtgtc
      181 tatgcttggg gttgggcccg caatatctgg gctgacagct taggctaagt aatttttagt
      241 agccataaaa gccaagcaat gcttggcttt ttttatttct actttcgtag cgaccttctt
      301 attgaggaaa aggcccatta agctttacag tggtccagtt caagaccatc gcaatcgata
      361 cccaaaccaa atagggagcc atcaaccaac tggatatcgg cgaatatgaa cgtgtcgcta
      421 ataagatggc caatacagat agccaaagtc caattgcttc aatcaaggcc cagtcagggc
      481 gatgcatccg aaaatacaga atgctccaaa taaaattaaa taagccattc acaataaaga
      541 gaatgaccaa catccgaatc gtttgttgat taggcgctcg gtgatacgcc atcacaaacg
      601 caattccagc acaaataaag atagtcgacc acatcggccc aaagacccaa tcaggcggtt
      661 tccaggacgg ttgctgcaga tttttgtacc attcacccaa ctcagtggcc cacgcccctg
      721 caagagctaa aacaatcccc cagatgagag caaaaacgat gggtttatag tgcgccaacc
      781 attcagaact catacctttg ccatacccaa tagatgggcc atgtctttaa agaagatctt
      841 gatgtgtgcc atggggtctt tgcggactaa tttcttgtgc atatatgact caaaagttaa
      901 tcgctgaaca tctttgtctt cacacatttt gacaaaacgc tcacggcgct tatcgttacc
      961 ataccaaaag cgttgcatga tgcccaagac caagaacact gtaccgtgct ccttcatgaa
     1021 gcgtttgcgt gcttttgcta aagccttaac attacctgtt tccaataact catggataga
     1081 ttctgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcccgatcgt tatcccattt
     1141 cttcaggggc ttcatgggca atggggcacc ttcgtgacgt aataattcga ccttctctaa
     1201 acctgttatc ttttttaggt cagcgactgc ttttcttaat gaaaaacctt tatcggcact
     1261 accagtaccg atactcatcg tattgccatg gggaaaaatc caaccataga aatcagggga
     1321 taaagtacct tgataaaaga catcgcagcg agagccgtca taaccctgag gaggcgcatc
     1381 gggcacacgc acaatttcat gataggcaaa gacataatca acatcttttg cacctggtat
     1441 ggcgctgcgg ccaaccccag actttgcacc atctgcccca ataatcgctt ttgccctaac
     1501 cgctcccctt tgatcttctt ctaagcgatt ggaagaatga atacggtaat gcagaattgc
     1561 tgtcccatcc gtgtcgcgag taatcgtctc aaagatacca gtgcgccgtg tagcaccatc
     1621 attggccgca cgaacgcgta accactcatc aaacttatcg cgatcgacca ttcccacaaa
     1681 tccaccatca atcggaatat ctacctggtt gttcttcggc gagatcatgc gtgcagaacg
     1741 tgcttttgct actaagagct catccggaat ctcaaaatcc ttgattaaac gcgggggaat
     1801 cgctccaccg catggcttaa ttcggccagc acgatcaagg agcaggactt tatgtcccat
     1861 tttggctaaa tcgccagctg cagttgcgcc tgcaggtcca ccgcccacaa caacggcgtc
     1921 aaaggtttca agggaactca tgatgatcct ccatgggaaa ttaaacgacc ggatattgat
     1981 gtattttgtt tctctatttg cactactcgc tcaacaggcc gaatgtaaaa cgaaagaatt
     2041 gcagcagcca taaataaaat agcttcaatg aagaagacac tggcgtatgc ggccactgga
     2101 tctcccaata ggtagcgtgc gagatcgctt gcgccagtcc ctaagatccc acctaagcca
     2161 aaggcaatcg cctgagcggc accccagacg cccatccgca cgccctcacg tttctccttg
     2221 cccaccccag caaattgcat catcgaacca attgcggcaa tggagaatgc gccattagat
     2281 acacccaaga taaataccgt gcccagaaat ggccaacttg gtcctactat tcctgacatc
     2341 accaaactca gcatcgccaa ggctgatgca atacagcccc caacggtcca catccgcaaa
     2401 gaagagaggc tgggactnnn nnnnngcatg ctgtaatcca gataggctgg tggtctctcc
     2461 tggtgtcaag ccaaaagcag tacctgcaaa cggttccaga attaaatctt gcgagctata
     2521 cgccagcatc gagatgaata caaagatggt gaatcgacga acccgtgact cctgccatac
     2581 atcagcaaga gcttctttaa atcgcaattt tgcttgatta ttattgggtg ttatttcttt
     2641 gctaactttt ggctcaagtc catatagagc agtaaagcta agcacgacag caatacatga
     2701 aacgcttaag gaaaccagta ccaaacgttc aaatgaaaat gggtctagta gtcggccact
     2761 tataccagcg gtcatggcaa aaccaaaaat catcatgagc caaacgcaac tcgcagcagc
     2821 agcttttcgt ttctcatcca ctcgtttcgc taacaacacc aacaaagccg taccgctagc
     2881 gcttacacca agtccgatca tcatgaatgc cacaatcgct agagcaatcc cccaggctag
     2941 gttttcactc atgagcccca cggctatggc agccaatacg ccaccgctgg ccaataccaa
     3001 catacccccc tgaatccaag gagtagatcg accgctctga tcagacccaa accccatccg
     3061 tggtcgaata aattgcacta aataatgcat cgcaaccaag aggcctggga gcaaagcggg
     3121 aagggctaac tcaatcacca tcactcgatt taaaaccgat gtagtcaata ccacgacggc
     3181 tcccaacgaa gcctgtataa gacccaagcg agcaatagca atccatgtaa gcttgtcatg
     3241 gtggagtgcg gcgctattca tgttcatcct taactcactg caaacgcgct aaccatcatt
     3301 ccactaacaa agagagggac gccaaaacca cttaaaaaca aagcgcgctt aattggatcc
     3361 cctaagaaat accgcatcat gatcatctgg ccaataatta aaccaattac tgctaatgca
     3421 tgaaaaggca tagcgagcca aaacaaatat ccaacaacaa tgcactgtgg cacgatcatc
     3481 atgatgcaag cattccatgc agcaccctgc actcctaact gaactggcaa agaacgcact
     3541 cccatttgcc ggtcaccttc aattgctttg aaatcattca atgtcatnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntacgataa
     3721 tccgcaagca gcattacccc accagccgtt ttgctttagt cttaatggcg gagcactata
     3781 tgcccatgct aaggctaagc caattagggt tgcgacaaaa gcccatggac taatgaacca
     3841 tcccaacaaa agcgataagc tagtccaaat acaggctaca tataagcccg aacgcccggg
     3901 catcctacca gaggggattg ggcgctgtgg ttcattaata gcatcaacgt ggcgatcaaa
     3961 ccaatcgtta atggcctgac tggtggcaca aaccatcggg cctgccaaga ttacaccggc
     4021 taatagaaga tgccattgat tgaataaaga ttgccccgaa gcaattacgc cacaggaaaa
     4081 agcccacatg ggcggaaacc atgtgattgg ctttaacaac tccaaaatgg cagatggagt
     4141 tggccacgag cgctcgctcg tctggtttaa gaccgaagtc cccatttcca caatccctca
     4201 gttgataacg gcaataccat taagatgtgt tccaaaatcg ccagcgataa caaagtggcc
     4261 aaaatggtgg atgatgcgat ttgatatggc gttaccccag tagcaaatgc tgtctgccac
     4321 agtggtattg caaataaaca cgccatcaaa atagaaactg gcaacaaggg gttcatcgcc
     4381 cgcttggtga aatatgtatg aatgtacttc aggtgcactg gtaaaaattt ctcattgaga
     4441 ttgagcactc ccaagaaaac attcagtttg gcactttggc gcattgccca caaaataata
     4501 aaggtccata aaccaattaa attagttccg ccccacgtca ccagggcaac agcagcgccc
     4561 aaaacaagta aggctaactc atgatgggca atggtctgaa acgcataaaa ggcttttcgt
     4621 aaaccacgac actcaggagg gcaagcaatc cgtctagacc cagtcacgta gccaagtaaa
     4681 aatccaattt cttgccaggc ccatacaagg agtgcacagg taaagccgca ataggcgccg
     4741 gcaactgtat ttaaattggc actagtcttt aacccgacca aagcaaacaa aagaacaata
     4801 ccgctcatcc aaaaggtagt ttgatgggta gatcgtggtc gttgattaag gagcaaaatg
     4861 atgcccgtcc caaaccacca tgcaaagatg gtgtagagca agggaaaggc aaaagtgctc
     4921 atgctcattg tctattacca gcttggcgcc aaacgaacat tgcggggaag ctcgtggtta
     4981 cgtactggca agaaaaatgc tcgggcaaag actaggccaa cgcgaatacc cataatcggg
     5041 accatcattt tggcaaccag accacccttc gccttataca catccatttt ttggaacaag
     5101 gcaagcatct ggttcatgca ttcataaaat cttgggtcgt ctaaattgag cgtaatcgga
     5161 aacacttgct tagtaatttc attggtaatg tgcaagacct tcttgtcata atcagtagga
     5221 gagatctgca ttgctttata aagttgtgga cgggaatgat ctctgacgta catggtgctg
     5281 tataccgcta agcagaaaaa ccggatccag tatttgttga gccccgtaag aagattggga
     5341 ttggcgcgca tgattaatgc aaatgcctcg ccgtggcgaa actcgtcgtt acaccatctt
     5401 tcgaaccaga gaaatattgg atgaaaacgt aactccggtt ttttctcaag ctcacggaaa
     5461 atggtgatgt aacgagcata accaatcttt tcagataaat acgtggcgta ataaatgaac
     5521 tttggcttaa agtaggtgta tttttttgct tttgctaaaa atcccaaatc aatcccaata
     5581 tcaaagtctt tcagccactg gttaatgaag ccggcatgcc gtgactcatc tcgcgccata
     5641 tagccataaa gcaatttcat gtcttcattc ttaaccttac gcttaatctc ggcatacaaa
     5701 atacaacctg agaactcaga ggtaattgag ctaattaaga aatcaacaaa ctcatcatag
     5761 agttcagggt caatattgga atagttttta tccatattgg ctggacgttg aaagtgatca
     5821 gaatttatat ccgcagcaaa ctcggccatc aatacattcc attccgaacg aagcgcatca
     5881 atatttgttt tatcaacctc ctcaaaattg gtcgtataaa aacgagggct cagaacagcg
     5941 tcttgcaaag ccattttggt ggactgattg accttggcag ctgctggtat ctgatcacgt
     6001 aatagacttt ctgcggattc catactcttc tccaataatt aacggtgttg tgtttgttgg
     6061 ccatctacac ttagtagacg ggcaaagtgt tttgcattaa tcgctccaaa agactctagg
     6121 tcaatcgtgc gccccgtact ggggtccatt aatgtcagac gaccatcttg acgggcaatt
     6181 aattcaaatg ggggtttact atcaatatca cgacgcttgc gctcttgcgc caaggttctt
     6241 aaagcgcctc gtacaaatcc ggcttcgcca atgattgaat caatttgctt accagtttgg
     6301 tagtcaaaaa cggcaataga gccatctggt cgatcctcaa agcgcaactg ctttacgtgt
     6361 tttgctggtg catctggata acgtgcgctg acttgagatt gtgtaataac ccaaactgta
     6421 aacaatgttg ttgctgcaat acctagcatt gttaatagcg tgctacgaga aaatctaaaa
     6481 agagtgatct tcatacgcta gattgactca cctttgccat tgcgatgcct tgcccatagg
     6541 agtgagttcc ggattcactc tgaatagaac gcttatctaa agagtggagg tccacttgat
     6601 ttattttgga ccaagcatct gcaaacagac tagcaacatg cttaccatcc gctacgcacc
     6661 gnnnnnatca tgggctcagg atgcgctaat ttccaagcgc gcgcatgagg ccataaatga
     6721 agatacggaa tcttatcctc aggatttaag cgaatcggaa tatcggcact cccatcctta
     6781 taaatatgaa caccagcctg agccaaacgc ttgagaggta aattaaaagt gatggttagt
     6841 acgatgccaa tccgcatcac cagacggcga ttcgtcaagg tgtacatggt tgtattgact
     6901 gaccaatatg ccaacaagcc aattaagacc aatgcagcaa tagagagacc taaagcccaa
     6961 ataaaacctg gccattcctg actccaacca cccacagact gactaacagc gattcccttg
     7021 agcaaaacca ttacaccaaa atacatggca aaccaatgta tatgaaaggc atgcattgca
     7081 aaggctttga gatcggggct tccctgccaa atgatctgct cattacttgg caatggctct
     7141 ggtaagccag gttgagcctc gaactcgtgc tctggtgctt gataaagagt gctcatgatc
     7201 attaaaccaa tggctcactt ctttcgggtg tggcatataa ggtacccgct ccgaagtagg
     7261 ccatgatctt ttcctcttcg agcaaggtaa tctgttcaga actcttcgtt gcaggaacac
     7321 ttgcaaagtg tctacccaaa accgcttgaa ccgaaacttg attacgtgca actcgggcaa
     7381 agttcatagg taagagaacg cgtctgccgc cggcttctgg agtggtctca atttcgagat
     7441 accgaatcat catctcggca cgatctaccc agatctcttt aatggttcca ccacgcacac
     7501 catcggcgcc aactacattt aagccacgtg gatcaatatc ttgacgagcg atactgaatc
     7561 cgcccaaaat gcgcatgggc actatccgag gatccccctc tgaggtatgg tcagggacat
     7621 cagcacgatt agcatatgca ccagggccaa ttccatcgag catagggtta cctgttggct
     7681 cgataggtga tccattcaat cgatttgccg gtcttgcggc aagacgctca agcggctcat
     7741 cccgcggcac agagacgtca ccaccaaatt gggtcttaaa ggttttaggc tttggcattg
     7801 cagcaatgcc ttcggcgcga cgcaccttac ctgcttgatc gtattcgaga ggaaagccct
     7861 cgcgcttact ctcaatggtg aggtaataaa ttaggatggc aaaaaagacc caaaacacgt
     7921 acaaggccaa ttgtgcaaca tcgacatatt gtgtaattgc tccagttccc atgatgaact
     7981 ccccttttaa ataatgaatt agcttggaaa ctcagctaat ccaaacttcg aatccgaatg
     8041 cattggcatc gatcgcttca tcacgaccaa tggaccaacc gctactagtg ttgcaaacaa
     8101 taaaaacaac tcaatgtgat acacaaagct ataaccggtt ccaggtgaga ccaagacaga
     8161 accgaggttt ccagatacag ccaaacttga aactaaatcc cgaatcacac caccaagccc
     8221 gatcgctaaa ccagccgcag aagcgctcac agcgccccac gccccaatga ctaaaccgtt
     8281 catgcctaag gaatcaaagc tcatggcagt tgcgagagtg ctcacggcaa ataacccgcc
     8341 gccaaatcca attaagaaag ctccaatcct gaataaggta ggagatagca gtggctcaga
     8401 aaaaataacg caggtaaagg caaaaatacc gcacaaggca cctgctgcgg ctaagcgatg
     8461 tgagtcaatt gatcggttta atacttttgc agccagcata aatgccaaca gggctcctaa
     8521 ggcggtaagg ctagtgagca aggtagtagc gctaacagat aaacccaata cctctgcacc
     8581 gtaaggctcc agaatgatgt cttgcatact aaatgcggca gtacccatgc caagagcgac
     8641 tagaaaccga atgaccttat cttgcttagc aaattgtttc caggagtcct gaaaaagaat
     8701 aatcggctcc atcgctaatt gctctctggt cttaggctga cgcgcttctt gtttccataa
     8761 ggcaagaaga tttaacacca tggttaaggc tgctgcgccc tgcaccactt gtatgagtcg
     8821 caccggagaa aaatctgcca agagcaatcc gaacaatatg ctactaaaga ccatgcccag
     8881 caggaacatg acgtacatca aagccacaac acgcggacgc gactcagcac ttgctaaatc
     8941 agaagcaagc gccaaaccag ctgtttgggt cacttgcata ccagcnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa agctctaaaa
     9121 ggagcaaata ctaaaggtag cgcaaccatc agtgcgacaa gactagctgc tacacccatt
     9181 tccacaatca tgacgcgatt cagagtacca atcaacatga ccgtcgccat cccaaccgat
     9241 acttgaaata atgcaaggcg taaccattta cttaaaggca ggtctttggt tgccacatca
     9301 gagaacggca tcaagcttgg cgagatacga cgccatgctt ggaaaaggtt gatgttgagg
     9361 gatttcatga tttgcgaaga gccatcgcct gagaaatata aaaaccgcgg gcaatctttt
     9421 gcgtacttgc nnnnnnnnnn nnnnnnnnnn ggttcaataa atggtgcgcg atcacccctt
     9481 ggaaataatc gtcccacagc aatcatggtc gataaaaagg gattcttggg tgcgaatgta
     9541 aatgcgatgg tatttcgagt acgctctgcg agtccagcca atgctttagc aatatcatgg
     9601 cggtgataat gaatcattga atccatgcac accacatgat caaattgacc caaactggga
     9661 tccaacatat cgcccgcaaa gaagtcgatt gagccattag gcaaatcact aggtaaacgc
     9721 tctcgggcta actcaattaa ggttggagaa atatcggttg ccatcacccg agcgccccgc
     9781 tttgctaatt ccacagcaag cgcccctgtt ccacaaccgg catctaaaac acgttgaccg
     9841 ctaagatcac tcggcaagta cgcaagtaat acattacgca tttcatcgcg cccagcacga
     9901 accgtcttac gaataccact cactggtgca gtcgaggtta acttagccca tgcatcaaag
     9961 gcagtgcgat caaaatactg ctctagctga ccgcgctttt cgagatacga atgatgttgc
    10021 atatccattt ccgattcaat caaatcctaa gagatcaaaa atatcgcggt ccttcattgg
    10081 aaccgctggc aatgggtcag ttccagccca aagagcagcc gcaagtcgta aatattcttt
    10141 ttgaaccaga tcgagctcgg gcgattgctc catctcaaac aaggtggact tttttaagcg
    10201 gctacgacgt ataacatcca aatctggaaa gtgcgccatg gtctttaggc ccaccttctc
    10261 attaaattta tcaatttgat ctgtagccgc actgcgattc gcaatcacgc cgcctaaacg
    10321 aacgttatag tttttggctt tagcaccaat cgcctgaatg atgcgattca tcgcaaagat
    10381 agaatcaaaa tcattcgcag tcacaatcag tgcccgatcg gcgtgctgca agggtgccgc
    10441 aaagccaccg cacaccacat cacctaagac gtcaaagatg accacatcgg tatcttccaa
    10501 gaggtggtgc tcttttagaa gcttgaccgt ttgaccaacg acataacctc cgcagccagt
    10561 tcctgctggt ggtccaccag cttctacaca cattacaccg ttataaccct catagacgaa
    10621 gtcttcaacg cgaagctctt ccgaatgaaa atcgactttc tccaagacat caatcaccgt
    10681 aggcatcaac ttcttggtga gcgtaaaggt ggaatcatgt ttcggatcgc atccaatttg
    10741 gatgacacgt tttcctaatt ttgagaaggc aaccgacaag ttggatgagg ttgtactttt
    10801 accaatccct cctttgccat acacagcaaa tacttttgct ttaccaatct ttaaattggg
    10861 atccagttga acctgaacac taccatcgcc gtcaggtcgt cgacccttca tatcacttac
    10921 cggtatattg actacgttca ttcgtgagct ccaaagatat tgttcatggt taaagtgaaa
    10981 tcaacgccca aaatgggcct tagcgtcata cagcgtttct aaagaaatcg taggaagacc
    11041 acgttcgttg gcaaagcgtt cggtgttgcg tctggcctta ccgcgtacaa aaaacgggat
    11101 cttattgagc tccttctgag cctctggctc ccaaacactt tccatcgtcg gtacgccatt
    11161 cactacagcg gcactcacga cttcaacaga ctcttcagcc ttaatcggct cattggcaga
    11221 ggtcttagca tggccatgac ccaaatgcga tggtgcagca ccatcatgga attcaaaatc
    11281 ttctctaaac atcccaataa gatgctcttc taaacccatc atgagtgggt gaacccaagt
    11341 atcaaaaagt acgttcgcac cttcaaagcc catttgaggt gaataccgtg ctggaaagtc
    11401 ctgcacatga attggcgctg atatcaccgc acagggaacc tttaggcgct tcgcaatatg
    11461 gcgctccatt tgcgtaccca ggattagttc aggttggagt tctgtaatct tttgttcaac
    11521 ctctaagtag tcatcagaaa taagtgcttc aattccatac tgagtagcag cctcccgaac
    11581 ttcacgcgcc agttctcggc tatacgttcc caaaccaacc acttgaaagc ccatttcttc
    11641 agaggcaacc tttgcagcag caattgcatg agttgcatcg ccaaagataa atacgcgttt
    11701 attggttaaa taggttgaat caacggatag ggcgtaccag cgtgcccgtg aacgcttcgt
    11761 aagttcctca atatcacact gaatttcagc taatgtgcat acttcttgaa taaatgcttg
    11821 cgttgcctta aatccaatcg gaatggtctt tgtacttggc tgaccaaaat ttttattcat
    11881 ccactgtgct gcactgttcg ctacttctgg atacaaaacg acattgaagt ctgcctcatg
    11941 taaacgagtt atatcctcag gcttcgcact taatggtgcg atcacattaa cccgaacgcc
    12001 aatttgacgc agtaatttac gaatctccac gacgtcatcg cggtgtctaa aacctaaagc
    12061 ggtgggacct aatatattgc aactcggcgg tatgccagcg ggacgatgtg ctcttttctc
    12121 tcctggtggc aatacatact cttttccaag taagcgcacc atttgataga aagtttcagc
    12181 agcgccccaa ttttctttct tttgataagc cggcaattcc aatggcaaga caggaactgg
    12241 taactgaaga gcggcggcaa gaccacccgg atcgtcctgg attaattcgg cggtacatga
    12301 agctccgacc attaacaaat ctggtttgaa acgagcgtag gcagaacgag ctgcgtcttt
    12361 aaataactcg gcagtatcgc taccaagatc acgcgcttgg aatgttgtat aggttactgg
    12421 tggacgcttg ttcatgcgct caatcatggt gaacaataaa tcagcatagg tatcaccctg
    12481 aggggcgtgt aatacataat gcacaccctc catcgcggta gcaatgcgca ttgcaccgat
    12541 gtggggaggt ccttcatatg tccagagggt taatttcatc gttatctcct aggctgccaa
    12601 cttcgttttc cgcactaaag gacgtgcaaa aagctcggcg agatcagcgg cctgttcaaa
    12661 accttgaatc ggtgtaaaaa ccaactcaat ggaccacttt gtagtcattc cttcagcctc
    12721 gaggggattg gccagaccca aaccacaaac caccatatcg ggattggcgc tgcggcaacg
    12781 gtctagttgc ttctccacat cttgaccctc agaaagacaa acgtccttag gtagtaatgc
    12841 aatctcttcc gctagatgtt gacgatggat ataaggcatt cc
//
LOCUS       DDPQ01000040            7596 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_4711,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000040 DDPQ01000000
VERSION     DDPQ01000040.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_4711"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    4587..4647
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2159 a         1605 c         1775 g         1996 t
ORIGIN      
        1 ctacggcttt gatcatggtg gaggtggagc caacgatatc agcctgatca actacatgct
       61 gcggggattc ccgatgcaca aggattaggg cattgggatg cttcttgcgc atggcagcca
      121 gctcgattgc tttgaactca tcatgcacga tacaggcccc attccagagc aacatatctg
      181 ctccagtttg ctcctgaatg taacgaccta agtgacgatc tggggcccat aaaatctttt
      241 tgccttgctg atctaaatga tgaacaatag caagcgcgca cgagctggtg accatccagt
      301 cagcttgcgc tttaacggct gcgctggtat tggcgtagac aaccgttgtg cgatcagggt
      361 gtgcttctct aaatcgacga aagtcttcag cagggcaacc tagatcaaga gaacaggtcg
      421 cttctaagtc tggcataaat acccgcttct ccggactaag aatttttgca gtttctccca
      481 taaagcgcac accggcaaca ataagatttt tagcgggatg gttcttgcca aaacgtgcca
      541 tctccaatga gtcagcaaca aagccaccgg tggcatgcgc aagatcttga atatcgccat
      601 cgacatagta atgcgcaacg attacagcat ccttacttat tagtaattgc ttgatacgct
      661 caatgatctc ttgtttttca ttagccaaaa gaggggttgg tgtttttgcc caagcttgcg
      721 caatgcaggt tgccccagag gcatcttgtt gagggtagtc gtagaaaact accgggtttt
      781 ggtggtcaat cgcttcggtc atcccgaaat cttaacctgc aattgctttc gggatagaat
      841 tcagggctta gagaggattt ctatgaaacg catcatgtta ttggctaaat tgcatcgggt
      901 tgtagttact gaggcagatt tgcactacga aggatcttgt gggatcgatg aggaccttat
      961 tgatgcagct gatatgcgtg aatatgaaaa gattgaactc tacaatatca ataatggtga
     1021 gcgcttttct acctacatca tcaaagcaaa gcgaggctct ggagcaatct ctttaaacgg
     1081 agccgcagcg cgtaaggctc acgtgggcga tcatttgatt atttgcactt atggaccgat
     1141 tgatgatgac caatcaaagt cacactcacc caaggtcatt ttggtcgacg acaagaatcg
     1201 tattaaagag ataaaaaaga ttactgttta aaacggtagc tttgcatcag gctttacagc
     1261 cagtaaagcc ttcttaaact ctccctgaat tcgcttgatt gcttcatcag agtctgcctc
     1321 aaaacggagg accacaatgg gcgttgtgtt tgatgggcga gccaagccaa atccgtcagc
     1381 atattctacg cggactccat caatggtgtt gatcgattgc gagcttggga actgcggatt
     1441 agccttgatt ttctcaagca gaagaaagga ttctccttct gcacagggta attgaagttc
     1501 tggagtgcaa attgcattag gtaaggcatt tagtacttcg ctggggttgg ctactttact
     1561 aaggatctca agtagacgag cgccggtata aagcccatca tcaaagccaa accaccgatc
     1621 cttaaagaat atatgcccgc tcatttcacc tgctagaggg gcgccagtct ctttcagttt
     1681 ggcctttacc aaggagtgac ctgttttcca catcagagct tggccaccgt gctgttcgat
     1741 ccaaggagca agattgcgtg tgcacttgac gtcatagata atctgcccct tagggtggcg
     1801 gctaagcaca tctttagcaa agagcatcat ttggcgatcg gggaagatga cttgaccgtc
     1861 tttggtcaca acccctagac gatctgcatc tccatcaaaa gccaaaccta gctcattatc
     1921 cgtattagca agattgtgaa ttaggtcttg gaggttctca agatgggcag ggtcgggatg
     1981 gtgatttgga aaatttccat cgacctcaca aaagagttcc tgaacatcac agcctagcgc
     2041 tctgaacagc tctcctgcaa aagcaccacc cacaccattg ccgcaatcaa ctgcaatctt
     2101 catgggtctg gcaaggcgaa catcacccac aatgtatttg aggtactggg ggaaaatatt
     2161 gaaggtggag cgctgaccag ttccctgatg aaaatgctca ccctcaattt gtttgcgcaa
     2221 actctgaatc tgctcgccat aaatagccgc accacccaga accattttga acccgttgta
     2281 atttggagga ttatgactac ccgtaatcat gattccagat ttgggctgga tgctacccaa
     2341 agtttgatta gcagcaaagt aaaccatggg ggtagccacc atgcccagat caatcacatt
     2401 gatgccggtt gacagtaaac ccttggttag cgcctcaatc agttctgggc ccgatagacg
     2461 accatcgcga ccaatgacca catctgtttc accaagatcg cgcatttgac tgccaaatgc
     2521 ctgaccaatc gcatgagcga tttgtggatt gagggtctca ttaataatgc cccgaatatc
     2581 gtaggcctta aagatggatg cgggtacttg cataataatt attgttttaa atagttaatg
     2641 cgagcagagg ttacagagtc aaaagcttca ttatttggtt gagacccctt gagcgcttct
     2701 tcaatcggcc gagctaagac tttgcctagc tcaactcctg gctggtcgaa tggattgaga
     2761 ttccacagag ctcctaaaca aaatgcacgg tgttcatata aggctaatag agcgccgagg
     2821 tggtatgcat ctaactttgg taacgcaatt aaattacttg gtcgcttgcc ggcatagctc
     2881 aaatttggat cggaggactc tttaccatga gcaagagctt gggcctgggc taagcaattg
     2941 gctaagagag cattgtgatg ctccttggcc tctggtaatg agctcatcgc ctccttcacc
     3001 ataatgaaat ctaccgggat gatctcgggc ccttggtgaa ggagttgaaa gtaggaatgt
     3061 tgtgcattgg taccggcact tccaaaaacg atgggagaac ttgcttttac atgcttgcct
     3121 tgtctatcgg tactcttgcc attactttcc atatcgagtt gttgtagcca aaatggaagg
     3181 agttctaaag catgtgcata gggcaccacc gcatacgcct tagagtgatg tttagtttgt
     3241 tgatggtaaa gcgcaagagc aagtaaaacg ggtagattgt gagagattgg cgtgcttgca
     3301 aaatgctgat ccatcgcatt ggcaccggat aaaaattgtt tgaaggtctc aaaaccatac
     3361 tgcaaagcaa ttggaagacc aactgcagac cagaccgaga agcgtccgcc tacccaatcc
     3421 caaaacggga aaatattgtc tgcttgaata ccaaaggatt cggctgcttt gacattacag
     3481 gtaacggcaa ataaagagtt ttggatctgc gagcttgtga gattcttagc cttaaaccaa
     3541 ttgatgatgg tctttgcgtt catcatggtt tcagaagtac taaaggattt agaaacaatc
     3601 aataccttag tgctattagc ttgtgcgcgc tccagaatat ggcttaactc agcgctatcg
     3661 acgttggcag caaaatgaac tcgcatgcca cgatttggtt tgttcgccga gtgagctaag
     3721 gcctgtactg ctaggcgtgg tccaaaatca gaaccaccaa tcccgatatg gattaaatca
     3781 gtcaccccta cccatttatt acataaaccc tccatgcgtt gccaaacttc agcaattgct
     3841 ggcattacat cattgccctt ataaagcatt ggatttttat ttagatcacg cagggtggta
     3901 tgcagggcag gtcggtgttc tgaggtgttc accagaccac cggttaataa ctcagtaatc
     3961 tgccctttga gacctatttg atccgccttg gtaaataggc tagcccactc tggttgacta
     4021 attccttgat aagcaagatc aagggtaagg ccggcagctt gtaatgaacc aaagaccgct
     4081 ttcgagggat tggcactatc gctttgagca gacttggtag acatgggtag attttatcaa
     4141 taagccctta aaaagcctat ttaaggctga agtgttacga ttttgtcttc ataaacagca
     4201 gaacaatatg gaacgattag attgtgtcgt agttggggct ggcgttgtcg gcttagccat
     4261 cgcccgcgag atggctttgc agggtcatga gaccattttg ttagagcgcg agtcctcatt
     4321 tggaacaatt agcagcgcca gaaatagtga ggtcatccac gcaggtattt attacccaaa
     4381 agattcttta aaggctaaat tttgcgttga aggtaatcgt cttttatatg agtattgccg
     4441 gggtcaccag gtgggcaccc agcgttacgg taaattaatt gttgctagtg ataaaaatca
     4501 gattgatgcc ttagagggac ttttctataa ggctcaactc aatcaggccc cagaaattag
     4561 catgattact gctaaggagg ccaagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnggg atggttgcct atttatctcc tctcatccgg
     4681 gcgacagtca tatccggtgg ctttgaattg gaggttggtg ggatagactc gatgcgcctg
     4741 caaactaaat atctgattaa ttgtgcaggc atgagcgcac ccgccatcgc tagaaagatc
     4801 gagggcctat ctcctgaaac gatccctcag gcctattttg caaagggtaa ttacttttcc
     4861 ctctctggca ggtcaccatt caaacatttg atatatccag tcccagagcc aggcggactc
     4921 ggagttcatt tgacattgga tatggcaggg caagcaaagt ttggccccga tgtggagtgg
     4981 ctcgacattg agtctgaaga gcaaattaat tacgccgtgg atcccaagcg gggcgagcgt
     5041 ttttatgagg caatccgcca atactggccg aaccttaaag acggagcact gcaagcggat
     5101 tactcagggg ttagggctaa gattgtgtct aaggaccaag ccgctgggga ttttaggatt
     5161 gatggcccag aggagcatga aatcgaaggc ctctataacc tctatggctt tgaatcacct
     5221 gcattaacct caagcctcgc gattgcgcag catttaagaa agataatggt ttagttggaa
     5281 atttcaaagc agaaagccta attccgtaac ttccttataa tctcaccttt aatggaagcg
     5341 tggcagagtg gttgaatgca ccagtcttga aaactggcga ggatgaaagt cctccgtgag
     5401 ttcgaatctc accgcttccg ccataataaa taacgtatga aaaatacatt ccatcataac
     5461 ttaaatattc cggttaaatt tatgccagtt tttctggaaa acgctccggt aatatcgatg
     5521 tacgataaaa gtttaatcaa taaagaattg atagactggt tgtcatcact acaaatagaa
     5581 attggtaaag cagagctatt tcatttaaaa tcaaatccag ccaatgaggg cgaatacaat
     5641 aaactaccaa ttcatttgga cgatgaagat tttgacaatc atgtaaaaat taattttgtg
     5701 tattgcaata caccatcaaa aatgaattgg tttgtttgta aagatccaag taaattaaaa
     5761 tgtctaaaaa ctccaacagg aactgattat atactagcag aggaagagga ttgcgagcta
     5821 gtatattcgg cccaagtagg acaaccaagt ttagtcaatg ttggtatgtt acacaatata
     5881 tcagcagttg attctgaaag gtattgtttt tcttttccat taataaaaga taacaaacca
     5941 ttgtgttgga accaagcaga agaaattttt aaagaatatg tatccttagt gtaatggcag
     6001 cattacagtc tccaaaactg tcggtcgggg ttcgagtccc tgagggtacg ccatataaaa
     6061 taagtgttga caacagcacc agaagagagt ataataacaa catgtacaaa gttatatgta
     6121 atgacagaga attagaaaga tttataacct taaacgaagc aatggcatac gccaaggccg
     6181 ttggggtatt tgtaactatt aaaggtccag attttgaagt agtgggacga tttggtgtag
     6241 acactgttgc aaatggtaaa tgcccggatg gtgctgttta caattggaac aaagctagcc
     6301 gcattggcag aacaaagcga gaacgagttt aaaattgcga gtgtggtgaa ataggtagac
     6361 acaagagact taaaatctct cggcttcggt cgtgccggtt cgattccggc cactcgcacc
     6421 aagtattagt aataagtaaa agaacaagga acggtcccat aatggtattg gagcggattg
     6481 ctaatccgtc gatcggcgaa agtcggttta agagttcgag tctctttcgt tccgccaaat
     6541 aactaaaagg taaaacatga aaccaaacaa gacatttaaa ctgagtaaaa caacaaaacg
     6601 catgattgga ttgatgaaag gcgcaactgc tgatcaacga aatcaataca agcgaatgat
     6661 gattgatgct cagttatgta gtgaaattgt tatcaaaact cctacacgtg ataaaaatgc
     6721 actacgtgga aacggcccta ctggatatca aactaacgat accggaactg ctagtactag
     6781 tactacttaa aaataagtaa tagcttgccg ccttagctca tttggtagag caactgactt
     6841 gtaatcagta ggtgcccagt tcgatcccgg gaggcggcac caaaaagacc ccggattaca
     6901 cttttacgtt aataaagtgc gtcattacag taacgataac tgtacggtgc gttggatcta
     6961 ccgcaaggtt ctctttagga acgacttgag aaatcacaaa ggcagggacg ttgcccgtct
     7021 aaatggaaaa gaacgtggac agagtaaccg ctcagtccgg ggctcatgtg gtgtgagtag
     7081 ccggacactt tattaaagta catttagctt gaacatagtt tgagtagcaa ggaccggcca
     7141 ccatcagcgt gatgaaaaag gcttaaatgt attctaataa agtatgcggg attagtttaa
     7201 tggtaaaaca gcagatttcc aatcttcggt cgagagttcg attctctcac tccgctccag
     7261 tttttattcc ccgttagcac agttggtagt tgcgcgacac tgttaatgtc gatgtccgtg
     7321 gttcgagccc acgacgggga gccaatttta tgcaacggtg gcagagtggt ccaatgcaac
     7381 ggattgcaaa tccgtaaaac cgtgagttca aatctcacct cctactccag tatgcccctg
     7441 tacgctaatt ggtagtgcgg attctctcaa aaggagttgg ctgtctgttc gaatcagacc
     7501 aggggtacca atttatggaa ggtaatgcag cggggatggt cctgcgactg gccttgaaaa
     7561 ccaggttctc agaaatggga tggggttcga ctcctc
//
LOCUS       DDPQ01000041            4629 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11395,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000041 DDPQ01000000
VERSION     DDPQ01000041.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11395"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1398..1563
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3634..3670
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1156 a         1172 c          958 g         1138 t
ORIGIN      
        1 accggtgcaa acgtacgccg atgttcttca caaggtccgt actgcttgag tgcctgcata
       61 tgagcctctg taggatagcc catatgctga ttaaatccgt actgtggata ctgcgtgtgc
      121 agcgcttgca tttgttggtc acgagtgacc ttggcaataa tcgatgccgc tgaaatagcg
      181 ggctctttcg catcaccctt aataatggct tcactagcaa tcggcaatat tgggcaacgg
      241 ttgccatcaa tcaaggcctt actgggccat tctcccaaac gctcagacaa cgcctctatc
      301 gcacggcgca tcgctaacat agtggcttgt aagatattga gtgtatcgat ctcgcttgga
      361 ctggcttgtc caatccccca ggctcgtgct ttttgcataa tttctgcata cagctgctca
      421 cggcgggcgg gcgataactt tttagaatcc ctcaaaccta tgattggctg attgggatcc
      481 aacactaccg ctccagcaac cacagagccc actagggggc ccctgcccgc ttcgtccaca
      541 ccgcagatcc aaacagtgct catgggtgcc tatctagacc gtcgattaag ggtttgttca
      601 atcacttgcg caaccagtaa accagtaggt ctttttaaac tcaagtgcaa ttgctcaaaa
      661 cgggtctcaa gtgctttcac tgccgccggt cgatctaacc acgataaaac cgcatctgcc
      721 aaagcctctg gctttgcctc atcttgcaag agctctggca ctacaaactc accacacaaa
      781 atattaggta gccccacata gggtagatag ccctgtcgct tcatgatctg cgcggttaac
      841 caaggcacct tatacgaaat caccatgggc tttttccaaa gagcagcttg caaagtggct
      901 gtgccactag caatgaggac aacatcagca gcagccaagg caagatcggc ctgaccatct
      961 aacaatatca gatcaatttc tggatggtcc atcaataaat ttgcacggag ggattcaatc
     1021 gcgggtcgca aatgtggggc agcaattggt actacaaagt gaagcttctg acctactaga
     1081 cgttttgcta tcagggcgat tgtttcaaag aaaacagggc caattaactc gatctcagat
     1141 cgacggctgc caggcaagac tgcgatcaca aggccttcaa gcccgccatt aatttttttc
     1201 ttggccgcta cagtatcaac aatttctgga atttcactgg ccatggggtg ccccacataa
     1261 cttgcggcga taccagcttt ctcataaatc tcaggctcaa atggaaacaa gcacaagaca
     1321 cgatcaactg cttgtttgat cttcttaata cgactgccac gccaagccca gatcgagggg
     1381 gaaacaaaat gaatcgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnngatcgca gacgcaaaat agctggtaat tggcgtaaag cctcaacata acctctgacg
     1621 cttaaggtct ccatcggcca aagcgaccta aatccctgtg cttgcattgc ttgaccaccg
     1681 ataccgtacg catccagccc agccatcgat ggtatctgat ggagtgcacc aaagattggg
     1741 gctgcgatca ggtccccaga tggctctccc gcaacacaag ccagcttagg caaaaccaac
     1801 taccgaataa tgccgcgcgt cgatgcggct aagaattgat gaaactgact gagagcctct
     1861 tgcgtctgct gatccgaatt ctttgtagcc atcgcctgga ttgcaagctt agcttcctca
     1921 aaactgaggc catctttata aagaacttta tacgcagctc gtagctccaa cacacactct
     1981 ggcgaatagc ctcgtcgctt taggccttcc acattaatgc catgaggctg ggctttatca
     2041 ccagcagcca tcacaaaggg aggaatatct tgcaccaagg cagacgcacc acccagcatc
     2101 gcatgggcac caatccgaac aaactgatgg acnnatggtg tgattaccaa tctgacaatc
     2161 atgcgctatg tgcacatatg ccatgatcca gttatcgttg ccgatgctag tgattccctt
     2221 atcttgagaa gttcctgtat gaatcgtagt gaactcacga atcgtattgc gatcgccgat
     2281 gattaattgt gttggttcac cacggtactt catatcttgg ggctcaccgc cgatcgctgc
     2341 aaagtgacca atccgattat ccttacctaa ggtggtgtgt ccttcaacaa cgctatgcga
     2401 ccctaccgtg caaccggcgc ccattctgac atgagcccca atcactgcat aagggccaat
     2461 gaccacatca tcggccaatt gagcattgct atcaacgatg gcagaagcat gtatcttggc
     2521 catcagtttc ccttcttgcg aacggcacag gtaatggctg cctcggctgc taactcgtca
     2581 ttaaccgtcg ccttgacctg aaacttataa atccctgctt tgccacgctc aaagttagca
     2641 tgcaatatca attgatcccc tggcaagaca atacgcttga agcgcgcgcc atcaatacca
     2701 gcaaaatagt aaacatcttc gggatcatgc gcctcagaga aggtcaataa ggcggctgtt
     2761 tgagccaagg cttcgataat caatacgcct ggcatcaccg gatagtcggg aaaatgcccc
     2821 tgaaaaaatg gttcattcat ggttacattt ttaagagctt tgattgtttt tccaggctca
     2881 atctctaata cacgatcgac caatagaaat ggatagcggt gtggtaataa ccgcaaaatt
     2941 ttgttgatat caatcgctga tgtctgactc ataaacgctt ccaatgtagt aatgcttcct
     3001 tgttaccagt atcaatctat acaaccttac gcttctatgc ctttgggtcc tttttttcaa
     3061 gatcccgtat acgttgccgt attttatcta gtccgcgcag aatggctgca ttcttttccc
     3121 acaaggcatg cggcattgat ggaaagacgc ccgttaaatg ggtgccgggc tctgtaattg
     3181 agcgaataac tgcggtaccc cctgaaatcg tagttcggtc agcaatagtt aaatgccctg
     3241 caaaattagc attgccacca atcacacagt agttgccaat agcggtgctt ccagcgatgg
     3301 ccgcacatcc cgctatgacg gtatgcgccc caacgctaac gttatgtgcg atttgtacct
     3361 gattatcaat tttgcagcct tgtccaatcg tggtattcgc catcgcaccc cgatcaatcg
     3421 tcgtacaggc cccaatctca acatcatttc cgatagcaac cgcccctgtc tgtgggatct
     3481 tgacccattc accgccttga gcagaaaaat caggggcaaa gccaaagcca tcggcaccaa
     3541 tgaccgctcc actatgcagt atgcaacgct cgccaatctt acatgccgaa tagatggata
     3601 cattggaata aatcagggag tcatcaccaa tgannnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn acaaagggtc caatatggca agagctcggg atcacagcgc ttggatcaac
     3721 gactgccatt gggcttattc caggggaatg tattggagca ctctctttag cgaaccattg
     3781 cgccattcga gcaaaagtgg cgtacggatt tttagagagg aaataaactc tactctcaga
     3841 acccttcccc tgctcctgta aaaagctcag atcagtctga ctaagaatta aagcgcccgc
     3901 cttactttct atcgcctgct gacgataaag cggatttgac aaaaaagaga tttggtcggc
     3961 ctgtgccgtt tccagaggag ataacccccg aagtggtcgg gaaccatccc ccaccaagct
     4021 tgcttgaaat tgttcggcca gctcgatggc ggtaggcata agattactta aggttattaa
     4081 ggaccttaat cacttcatcc gtaatatcaa gtcttggatt aaggtatgcg ggggattctt
     4141 gaaaaatgat atcaattttt cgttgctcag caatttgacg gagggcgacg ctggccctct
     4201 ccgcaatttt tgccctttct tcagcattac gctgagcggc atcatcattg agctcacgat
     4261 tttttctttg tagatcacga tcaaagtcag ctagctcccg ttgcttacga attcgctctg
     4321 cttcaggcat cacagcagca tcacgatcta gcttctcagc ggttgcttta atcttttggg
     4381 cgccatccct aatttcattt tggcgtttag caaattcatt ttggagtcta gactgcgctg
     4441 ctttagccat gtttgattca acaaaaatct tttcagcatt cacaaacgca atgcgcgcac
     4501 cagcatcttg ggcctggacc attgaaccga acagagtcag caaagttacc gtgcctaatt
     4561 tagcccaatt tgtcattaat attcttttca tcttttcttt ccttaccttt aaaacgccgt
     4621 gcctacctg
//
LOCUS       DDPQ01000042            5977 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5818,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000042 DDPQ01000000
VERSION     DDPQ01000042.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5818"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    666..772
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4990..5054
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1359 a         1416 c         1441 g         1589 t
ORIGIN      
        1 tgctggtcct tggcggctag caagatacgg gcaatccgcg ggtctagggg aagatctgct
       61 aactcttttc cgatacctgt cagttgcagt gcgacaccac cttgattgct ttcttcttga
      121 atctcaatcg ctccgagctc ttcaagtaat tgcatgccat cggcaatcgc gcgtccgagt
      181 ggtttatcga tgaaggggaa ttgattaacc ggtggcaaac gcagagatgc catacgaaga
      241 atcactgctg ctaatgagct tctgaggatc tcagggtctg tgtaagccgg ccttgctttg
      301 taatcctctt cactgtaaag acgaatacaa ataccatcgg atactcgtcc acaacgacct
      361 gcacgttgat tggcagccgc ttgggagata ggctctattt gcaattgctc tactttattg
      421 cgatacgaat agcgcttaac ccgtgctaag ccagtatcaa tcacataccg aatgttcggt
      481 acggtgagcg aggtctcggc cacattggta gctaagatga tccgacggcc attgcctttc
      541 tgaaacacgc gttcttgctc actgacagct tggcgtgcaa atagacttaa tagctcagga
      601 tgaaaacgtt gcaatacggg gtccttacgc aacgcttcag cacaatcccg aatctcgcgt
      661 tcaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgttgctc
      781 aactggatag agtcggccgc tcacctcaat cactggtgcg ctcttaccat tcatagcaaa
      841 gtgatttgca aagcgctctg catcaatggt tgcagaagtg ataatgatct tgagatcctt
      901 ccgcttgtcg atcaactgtc gtaagtaacc caagaggaaa tcaatattaa ggctgcgctc
      961 atgcgcctca tcgatgatga tggtgtcgta catctttaag agtggatctc tttgagtctc
     1021 tgccaacaat attccgtcgg tcatgagttt gatcgaggca ctcgggctgg tgtgatccgc
     1081 aaagcggacc tgataaccca catcctgccc aatgggggaa cctagctctt gcgcaatccg
     1141 tttggcggta cttgtggcgg caatacgacg gggttgggtg tgcccaatca aaccgccttg
     1201 gtggatacgt cctcgaccca gagccaaaca aatctttggt aattgcgttg ttttacccga
     1261 acccgtttct ccacagacaa tgatcacagg gcttgccaga aaggcttttg cgatcgtatc
     1321 gcgttgaccc gagaccggta aggcttctgg aaacgcaatc tcttttccgt gaagaggatg
     1381 ccagatcact tcgcaaggcg agctcattgg ggcctcatgg ggcatagaag gttgaactcc
     1441 cttccataat ttatttggta tctaagcgct tgcaccctat aattttctca ttatgtctac
     1501 ttccccagcc aatccctcca caaatcctag cttccccttt gtgggctggc ttcgtgaagt
     1561 tgcgccttat atccatgctt ttcgggaaaa gaccttcgtc atcgcttttg cgggagagtt
     1621 ggctcaagac tttgatcaac ttgagaacct gattgaagac atcgccatgt tgcatgccat
     1681 gggcatgcgg attatcttgg tgcatgggat tcggccgcag attgatgagc aactttcctt
     1741 acgcaagatc aagagcaagt ttggtaaggg ccatatgagt agctatcgaa ttaccgatgc
     1801 cgctgcgcta gagtgtgtca aagaggctgc tggcgaactg cgtttagata tcgaggcggc
     1861 atttagtcgg ggacttccaa atactcccat ggcaggttca cgcatctcgg taatctcggg
     1921 taactttatt actgccatgc ccattggagt cgtagacggt attgactatg ttcacacggg
     1981 gctggtacgc aaggttgact ccgagtccat caagatgtct ttggataatg gcaaattggt
     2041 cttgttatct cctctagggt tctcacccac aggacaagca tttaatttat cgtttgagga
     2101 tgttgcagcg gcaacggctt ctgcgttgaa gaccgacaaa cttattttct tatccccctt
     2161 cccaggctta caagataaag aagggaatgt ggtcaccgag ctatccattg ctcagttgca
     2221 agattatctc cagctctatc cagatctacc tagcaatatg cgtaacttcc tcaacaccgc
     2281 gattcgggcg gttcgctctg gagtaagtag aacgcatttc ttaccctgca atcgcgatgg
     2341 cgtgctctta gaagaactct ttacccacga tggtgttggc atgatgttgg ccgcttcgga
     2401 tattgagaac ttgcgtgagg ctacgcaaga cgatgtgggt ggtattttgc agttgactct
     2461 tcctcttgag gctgagggtg ttctggctgc gcgtggtcaa gacgtgatcg agcgcgatat
     2521 cgctcggttc tcagtgattg agcatgacaa ggtcttgttt ggttgtgcag ccctctttcc
     2581 atttgccaat ggtgttggcg agttagcctg tttagtggtg gatcccgact ctcaagggtc
     2641 tggggatggt gagcgcctgt taaagcgtat tgaggctaga gcaaagcgtg agggcattac
     2701 ttctttattt gtcttaacca cccgtaccga acattggttc ctcaagcgtg gttttgtgag
     2761 ggccagcgtg gatgctctac ccgaagagcg caagcaaatt tacaactggg accgcaaatc
     2821 catggtgctg accaaaaaga tctaggtctt agtctttggg tgggtttctg cgacccctaa
     2881 aacctttgaa ccctcctatt ttgtattcct acctataatt aggctcattc gttaaaaaga
     2941 ggtttgtatg gcaagaatgg ttcagtgtat taagctcaat aaagagtccg agggtctaga
     3001 ctttgctcct cttcctggtg aactaggtaa gaagatctgg aatcaggtat ctaaagaagc
     3061 gtgggcagac tggcttaagc atcaaacgat gctgatcaat gagaaccgtc ttaatatggc
     3121 cgatccgcgc gcacgtcagt atctactcaa acaggttgag aaatacttct ttgagggtgg
     3181 tgctgatatg gccagtggtt atgtaccgcc agcgcagtaa gtttattgat aggctttatt
     3241 tagaaaactg aactcgctga acaccctgct gtccacccac cagcaataga tctgccttag
     3301 cttgggcaaa gagtccaaca cagatgaccc cagcaatttg attgaggtct ctctctagtt
     3361 ggacagggtc tttgatatct aatccatgca catccaggat ccaagcgccg ttatccgtta
     3421 agaatggttg accttcttgc tttccagagg agatcaggcg tagtttggca gtaccgcccc
     3481 attgtttcag ggattgtgta atggccgctt gcgccagagg tatcacctca agcggtaatg
     3541 gaaatttacc aagagtatta acttgcttgg actcatcgca gatgcagata aattccttgg
     3601 caagacttgc aataatcttc tcgcgcgtga gtgctccacc accgccctta atcatatgac
     3661 cctgtgggtt aatctcatcc gcaccatcaa cgtacatcgg caagggatgg gattgtgttt
     3721 ggatcgactc cggtagctga ctggcatcga ccacatgaaa gcctaattta atcaggcgct
     3781 ctgtactcgc caaggagctc gataccactc ccgcaaagtg atcacggtgc ggggctaaga
     3841 ggtcaataaa ccagttagcg gttgagcctg ttccgatccc caagtactgc ccttttggca
     3901 ttcgctttag aacttcctct ttggcggctt gggcaacctg tttcttgaga tcgtcttggt
     3961 tcatgattgc aatcataccg aatgttctcg ctatcattta ctatttggct atggataccc
     4021 tctctcaact caagcgctat accacggtgg tcgccgatac cggcgacttt gagcgcatgc
     4081 gccagttcca gccacaggat gccaccacca atccctcctt gattttgaag gcggttcaac
     4141 aaagtaccta cagtaattta gtggcagatg taaaacgctc taaccctaag gcctccccag
     4201 cacaactgat tgatcgggtt ctagtcgctt ttggtggcga gatattgaag attgtgcccg
     4261 gtcgcgtttc taccgaggtg gatgcacgac tatcatttga tactgcagca accattcgta
     4321 aggcgcgaga gatcatcgct ctgtacgaat ccgcaggcat tgatcgtgct cgggtcttaa
     4381 ttaaattagc aagcacttgg gagagcattg ttgctgctcg ggagttagag caagatggta
     4441 tccattgcaa tatgacctta ctcttttctt tggtgcaagc tgttgcatgt gccgatgcca
     4501 aggcacaatt gatttcaccg tttgtgggtc gcatcagtga ttggtataag aaatccttag
     4561 gtgatcaatg gtcgactgct caacatgctg gagcaaacga tcctggcgtg cagtcggttc
     4621 gttcgatctt tatgtactac aagcactttg agattgctac cgaaattatg ggtgctagct
     4681 ttcgaaacac cagtcagatt ttggagttag cgggttgtga tttactaacc attagccctg
     4741 aactcttagc agagttacaa gcgagcaata ctgaggtgag cccaagactc attgcagaag
     4801 aagctcagcg tgctgatatg aaacagatac atcttgatca agctcacttt gaaaatgagt
     4861 tggccaagaa tccgatggct tctgagaaac tctccgaggg catcaagacc ttctgtgcgg
     4921 atatcgagaa acttgaggtc ttactcagtt aggatcgctc agttaagctt gctcagttaa
     4981 gctggtttan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnncaggtc tcgcgggtta agcggcgcat tccttcaccc tcggctccca
     5101 tcacaatcgc agtgggtcct ttgaggtcta cgtcatagat cgattgctct gcttgatcat
     5161 cggtgccaat caaccaaata ccaagctctt gcatctcacg catggttctt gcaagattgg
     5221 tcactgtgat gatgggcact acttcagcag caccgctggc cactttgctc acggtggcat
     5281 tgatgctcgc agagcgatct ttgggaacga tgaccccatg cactccagcg ccatctgcag
     5341 cccgaaggca agctcctaaa ttatgcgggt ctgtgatgcc atccaagacc aagagtagag
     5401 gtggatcatt ctttgcatca atgctatcga gtaactcggg tagagttcta gcaatcgaga
     5461 taggttctgc cagagccaca atcccctgat ggcggtcatg tccagctaga ttatgaagac
     5521 gatctgcatt ggctgagtag agtctcttac ctaagagtgg ctcagcctgt ttaatgaaat
     5581 ccgccatgcg tcgatcacgc cgtgctggat cgtaatagac cgattgcagg ctatctggat
     5641 ccatgcgtaa gcgggtctgt accgcatgaa agcccaacag aatctgtttc atttgcgctt
     5701 acctttggta ctcttgcgac ctgtcccttg agcaatcgag ggctgacggg ctgtgggatt
     5761 gcgtccgctc ttggctttcg ttttcttgtt tgctttgcta ggggccgcat cgcggcgtgg
     5821 ggctttctca tcggggcggg ttttcttatg cgccgctttt ttattggggc gaccagcatc
     5881 cgatgccaac accactgact taaaccgtgt gccatcacgc tcattcgttc catttgcttt
     5941 gaccagacca aactcaatct tacgggcatc taaatcc
//
LOCUS       DDPQ01000043           28229 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_1395,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000043 DDPQ01000000
VERSION     DDPQ01000043.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 28229)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 28229)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28229
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_1395"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    159..215
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2220..2285
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3574..3631
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4702..4721
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7168..7186
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7574..7650
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8383..8415
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10781..10806
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19379..19448
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21207..21231
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24197..24276
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7318 a         6435 c         6137 g         7808 t
ORIGIN      
        1 cccactaact ctactaaggc agtacttcct aatgcgttta agagtcgctc cattaatgcg
       61 ggatcgactt taatgtgacg ggtcatttgc aaggttaact ccaggacatc attttctaaa
      121 tcactaaaac agtctggatt aaatgtttct tggccaagnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatcta aagatagctt ctctcgaaca
      241 ttgccgatga agacattcca gcccctggca aacggttcgc tatgcaacaa catgcgatca
      301 aggttaataa actgaccgcc acgccgcttt ctgatctcag caaccagatc agcgggttct
      361 gataaatcaa ggggttgata cggtattaag cgggaatttg acatgattgt aggggttcga
      421 tgtttttctt tccctctagt ttagcgacaa acttgattaa taatacggct tatgaacgat
      481 ttaacgaaca agcccaagtc gctacctatc ctaaatattg ctgggtatca gtttgttcgc
      541 ttagatcaat tagagtcttt gcgggagtct ttacaagaat ccgcccttct tcatcaactc
      601 aaggggacca ttcttcttgc cgaagaaggt attaatttat ttctggcagg tctagaaaat
      661 gatattcaag aattcctaaa cactctacga gcagatcctc gactgcgaac ttttacaaca
      721 aaagatagtt ggtcgtcatc ccagcctttt cgtaagttgc tgatcaagat aaagaaagag
      781 attattcgga tgaatcatcc gacaatcacc ccagaaaaag gtagggcaaa atttattgct
      841 cctgagaagt taaagcaatg gcttgaccaa gggcatgatg atttaggacg acccgtagta
      901 atgattgata cacgcaatag ctttgaagtg gaatatggca gctttgataa tgctatgcac
      961 ttcaatatca ataaatttag tgagtttccg aaggcgattg aatcgcatct ttcggaactt
     1021 gctgataaga ccttagtaag cttttgtacg ggcgggattc gttgcgagaa atctggctta
     1081 tacctgcgag agcgtggctt agagcatagc taccaattgg atggcggcat tctccaatac
     1141 ttcgaagata ttggctccgc acactaccaa ggagactgct ttgtctttga tgagcgcgaa
     1201 actctagagc caaccctggc agccaagccg caaggtcgac tcaaacctaa aaaaacgaat
     1261 ccctaatcag atttaccgac taagttgaag tgctctacag ctgggggctg gataaaaaat
     1321 ggtccgacaa tgctacgcca ttctgtaaat gctggtgacc ctcgaaaatc aaccatatga
     1381 ttttctaatg tgtcccagta gatcatcaaa agatagcggt ttggggactc aatactgtgg
     1441 tttactttaa aaccccgaaa tcccttggct ggggcaatgg ctgtgcgaac tcctcgaaca
     1501 atagcctcat caaactcggc ttgtttgctt tcctctatac gtatgtcaac gacctctaaa
     1561 atcatctggc ttccttttaa aatctttact agaaacatca attatcatcg atttatcacc
     1621 gatttaatac aaggatgact attttctgta tgcaagcaaa accaaaaatt gtcgttttgg
     1681 gcgactatga gcaatgcctc caacgctttg cagattggtc atccattgcc gagcgttcgc
     1741 aactcagctt tcaccacgaa cctctttctg gagaggcctt atatgaagtg gtcaaagatg
     1801 cagaggtaat tgccttggtt cgagatcgct ctccctttaa tgctgaactg attgctcgcc
     1861 tccctaagct ccaatttttt ttatttacgg gtgctcgaaa caatcttcta gaccatcaag
     1921 tactgattaa tcgggggatt ccgatagcgt gcacgcctgg aggtccgtca aaagaaacaa
     1981 ccgctgaact tacatgggcg ctgatcctcg ccgctactaa aaatatcgtt gatcaaactc
     2041 aattactaca gcaaggtcaa tggcgaaatt ctgactctgt cttaccgatg ttaagcggtc
     2101 aacgccttgg cattattggt cttggcgcca ttggcggcat tgttgccaag gtaggagcag
     2161 catttggtat ggatgttgtt tgctggagcc ctaatatgac cccggagaga gctaaagctn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnagtct tcatttagta gtaggtccaa atactaaagg tctcatgaat aaagaacggc
     2341 tacaacttat gcgagcagat tctgtgttgg tgaatacggc gcgttctgca ttaattgtta
     2401 ctgctgattt aatggaagct ctgaagatgg gtcgccccgg taaggcagcc ctagatgtgt
     2461 tcgatataga gcccattcca caggactctc ccttgcgaca tactccaaat ctactcatga
     2521 gccctcacct cggttttatc gcagagccca tctttagcag ttttgcaaaa ggtatggttg
     2581 actcacttac agcatggctt gatggcaaac ccatcccctt accttttaaa taaatattct
     2641 ccataaagcc cacggtgcaa cacagtcctg gtcacttatt tatcacattc accttcattg
     2701 gtttatcggg ttttggtggt gtattgccat gggctagacg cactttagtg gaacaaaaga
     2761 aatggctcag cgcacaagag ttcaatgctc tcttaggcgt ctgtcaactg gtaccagggc
     2821 ctaatatcgt gaacttagcg gtctgcgttg gacagcgatt tggaggtcta cgtggagcca
     2881 ttgccgcagt ggcaggacta atgcttgccc caatgaccgt tgtcattctg ctggcccttt
     2941 tgtacgatca ctatggtcaa tttgaacagg tgcaaggaat gttacggggc atctcagccg
     3001 ttggtgttgg attgattgct gctactggtt ttaagatgct caaagaggag ctctcatatc
     3061 ttccaatgct gattgtgatt gcaattagca ttgtcatggc gactgtattt catttggcct
     3121 taggctgggt ggttgccatc actcttccct tagctctcct gtttgcctgg aataaagcca
     3181 aatgatgatc ggcttattag gcttatttat caagctatct ctgttttcct taattgcctt
     3241 tggtggagtc aatgcactgc tgcccactct ttatgatatt tctgtaaatc aagaaaaatg
     3301 gattgatgca gatactttta ttcattactt tgcaattgct caagcggcgc caggccccaa
     3361 tcttttaacg gtcacactca ttggttggaa tgcgtttggc ttggctgggg ctttactggc
     3421 cacccttgca gtctgctggc cctcttgtat ccttattttt tatttacaac gggtgctcgc
     3481 caatctccaa cacattcgtt ggaaaaaaac gattgagtat tcagctggtg cgctcgcggt
     3541 tgggcttgtt ttagcatcgg cttggcaaat tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtcgcaag catccgctgt acttaattgg
     3661 cttgggagcc ttgctcggat tactcggttt tgtctagaag gagagtgctt tgaaaatcat
     3721 attgcttcgg tatttatcgt taccttatct tagttgtatt gttattagta tctctcttta
     3781 tatcggcatc atccccaatg caaacgcgca agcgccctat cccaataaac cgatttcttt
     3841 tattgttccc tatggaacat cgggtagcgc cgatcttcgt agtagacaaa tcacccaaaa
     3901 aatgtctgtg atcatgaagc agagttttat tgtagataac aagcctggtg cggggggcaa
     3961 tataggtaca gaatttattg cccgcgctaa tccagatggc tacaccattg gtatgggtaa
     4021 ctttgcaccg ctttcggtca ataaatattt gtttgaggct ttacgctttg atccagaaag
     4081 tgatgtagta ccaattgttt tgattgaaaa gggtccgctg gtcttggttg ttaatgccaa
     4141 ctcaccattt aaatcaatca acgatatcgt gaaggcagcc aaggcaaaac ctggatttct
     4201 taccttctca tctggaggga tcggcggtac tcatcaactt tcagctgagc tgtttaaaca
     4261 aagtgcaggc attgatatga ttcatgtgcc gtataaaagt ggctcagcgg ccttaactga
     4321 cttaatggct ggtaatgtca atatgatgtt cacgcaaatg tactctgcga tcccaaatat
     4381 ccaggctgga aaattacggc ctatcgcaat tactagcaag acgcgatcat cgctattggc
     4441 caatgtgcca acctttattg aattaggtta ccccaaagta gaggtgctga actggcaagg
     4501 tttaatcgca cccaaaggga cgcctagggc cattatcgat aaactcaatg tagttgccaa
     4561 tcaagtatta aaagatccag ttgttagaga gcaaattacc tcacaaaata atgaggtggg
     4621 cggtgggact ccagaagaat ttgcggctct tattaaatcg gagtccctga aatgggctgc
     4681 agtaattaaa gcagcgggca tnnnnnnnnn nnnnnnnnnn natcgcacca caaacatgca
     4741 tcaaggccag gcctaagccc caggtgagct gacctcgctc aatgagagcc acaacctctg
     4801 cggagaagct agagaaggtt gtcagccctc ctaaaaaacc agtgatgata aaaagtcgcc
     4861 actctgaaga gacggaggga tggtttacaa agtatgcgac tgcaattccg acgagatagc
     4921 aaccaaatag atttgagatt aaggtaccta gcggcacaat cgccgaaagg ccagccgaga
     4981 ttgtattaaa ccaaactctc agtagagcac caagacctgc cccacaaaaa atagctgcga
     5041 gcgataggcc gctcagtgag agtcccattg ttgggatgat gaataggttc taactaaaca
     5101 catagccggt atgggcggca accaaagcaa acaaaatagc aagacttgtg gcagcagcaa
     5161 gcatcacaat caacgtaata atttttttgt tgatagagcc tttatcttcg ttatgccatt
     5221 cattcatctt tatcccctaa ttccagcaag actgtaaaaa tgacattatc cacgattgca
     5281 tttatctaac gaccgcggcc agccttacgc atcattcctt tgcctccacc aaagccctgc
     5341 tgaggctttc cgcctaagct ttgtgggccc ttgcccgttg gaggacgctg aggaacttta
     5401 ggggctttga ctgcgggttt ggctaggggt ttcttctcat cggtcacaat gggctcctgt
     5461 cgatatgatg tagctatgtt tactgactat tctatccaag caatcgcaat taatgctata
     5521 cccctcattt ttgccatcac aatccatgag gctgctcatg gctatgctgc aaaacgcttt
     5581 ggtgataaca ccgctttttt acttggtagg gttactttaa acccattaaa acacattgac
     5641 cccatcggta cagttttaat cccgattgcc ctggtcttgg caaactcccc ttttctggtg
     5701 gggtatgcca aacccgtccc ggttcgcttt gatcatcttc gtaatccacg tattgacatg
     5761 atttgggtag ccctagccgg tccgggatct aatttcatac aggctatttt ctgggcggta
     5821 ttttggatat tgatacaggg ctttggtatt aatgagccat tcctgatctc gatggcaaag
     5881 gcggggattt tttggaatat tggtcttttg gtttttaatt tgtttccctt gccacccctt
     5941 gatggcggtc gcatccttgc cggaatactg ccctatagac aagccgtgat gtttggcaaa
     6001 ctagagcctt ggggcttttt tatcgttctt ggtcttctct ttacgggtgt catctcgcag
     6061 tggtggatgg ttccattgag tgactttttt attgccatcg ttcgcaccct cacctcgccg
     6121 ctcagaatgg tgtttacacc ctaaaatcga ctgaggcaaa cttaataata tccttcataa
     6181 aattcaggta tgctttatag cacctaagca gtttttaccc tttggaggtt tacatgaaga
     6241 cccactattc tcgtattact cttggaacac ttgtttctgc tatttgccta agtatggcaa
     6301 ccccagcttt tgctcagttt gcgaaaccag aagatgcagt taaatatcgc caaagtgctt
     6361 ttgcactcat gggcgctcac atggggcgct tggcagcagt cgttaagggt gagacgccat
     6421 acaacaaaga tgatgtggtt cgcaatgcgg ccatcattag catgctatct ggcatgccat
     6481 ggcaagcatt tacctctgga accgagagtg gaaaaacgtc agggaaaaat gatgcacttc
     6541 ccgtcatttg gtctgagggc gccaagttca aatctgcctc tgagaaaatg atggctgctg
     6601 tagccgatct aaatagcgca gcgcaatcag gtaatcctga gaacctcaaa aaagcattgg
     6661 gggctactag ccaaacctgc aaagcctgtc acgacgactt ccgcaagaag taataagcgt
     6721 attacacgga ctgtactaac tgtagctagt taatcaggta atacagtcca agccctactg
     6781 ctataaaaat catcagggcg accaagcgct gtttccctgt atcaataggc tcggggggag
     6841 ctgaaccccc aaaagcctct tcgccattct tatctccggt aatcattggg gctactaaac
     6901 tttctttttt atagaattga taaaaaagaa tggcgcctat atgcaatgca accagtgcca
     6961 tgatgatgta ttgattaaag cgatgcatgc tcgttaaaaa cgcaacagtc gcattcgata
     7021 catatttagc gaatggcccc tggaaaaaga tgtcgtcatc agcaaagaga ccagtggaag
     7081 cttgtatcaa aaccgccaac aagagcgcaa tcaccgagag tgctccgagg gggttatgac
     7141 caagactgtc acccattcca cccctaannn nnnnnnnnnn nnnnnntacg catgccattg
     7201 catggcattg cccccaattt ttacaaagat gattcctaga acgacacaaa gggcaagcgc
     7261 ccaatgaaac aaccttatcg gcaaatccca gacgcgaatc ttttgtttca ccttatttaa
     7321 tttcaggtag ttctttttgg atgagcttca aaccggctcg tagcaactcg tcgtaacgtt
     7381 gtcgctcagc tttaatggcg tcggcatccc atgaataaaa tccttgtcca gttttcatac
     7441 ccaagttttt agcatccact ttttctttca ggcatttggc tacttcgcct ttgttgttaa
     7501 gagatgggta aatactggca cctgcagccg catgaatctc caaacccgcg tgatcgcgtt
     7561 gcaatacggg cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttttaacag taaccggcac catgccgcat
     7681 gcggtcatta aacggcttaa gctatctgca acaaggggtg atgttttttc accataaacc
     7741 acttccacgc atggaacaat atgtgcgggc ataaagaaat gcaggccaat cattctggca
     7801 gaggttttta agccttctgc aatttgactg atcggaaaac tagagctatt actagccaat
     7861 acagtctgag gattaacaac ttgctctaat tgagcaaaca agtcctgctt aatgtctaat
     7921 ttctctggaa cgcactcgat tactaaatca atttccgacc aatcaaactg attaagcgaa
     7981 tctaccaaag caattaagtg ggctcgctga ccatagccca tgtccagtaa tttagtttga
     8041 aagtaatgtg gcaagacttt acagcgctca gcatttgctt caaatacata tgtcgtacaa
     8101 ccaccgcgtg cacaaacaag tgcaacgtca gcgcccatcg ttccgccacc cacaatcaga
     8161 accttagtat ctgctggact aaatagcatc ttaccgacct ttcaagacgg gagcagttcc
     8221 tgtcatcgat ggaaccatga aatcaaaatc acacccaata tctgcttgcg ttacggtagt
     8281 ttgatacaaa cgccgatatc ctgattctgg aatagcgaga cgctctgctg gagatgcgag
     8341 cgcatcgatg cgtttgcgta tctcggcatc atcgacctcc agnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnngttcc actcatccgc gcatccgaga tacgaaccat atctttaaca
     8461 ccagcctgcg ctaatttttt agggatcggc agataccccg cctctggcat tcctggggcg
     8521 ccctttggtc cagcattttg caagaccaaa atatcatcgg cattgacatc caagtcaggg
     8581 ctatcaatcc gcaatgccaa gtcttctagt gagctaaata ccaccgcacg cccgcgatgc
     8641 tttaataaat ctgggctagc tgccgagtgt ttaatcacag cgcccctagg agctaaatta
     8701 cctctcaaga ttgccaaacc gcccacagga taaatcggat cgcttgagat ccgtatcacc
     8761 tttctcgctt tgctcacctt ggcgtcctca atctcctcgc ccaaggtttt tcctgaaaca
     8821 gtcaaacaat cgagatacaa atggggtttg agctctctta gcaccgccat cagcccccca
     8881 tcctcataaa gatgttccat gtaatgagcg ccgctaggtt ttaggtcaac caaaacagga
     8941 acatttctac ctaattgatc gaccatatcc aaatcaatct tgatgcccaa gcgagctgcc
     9001 acggctgtaa tatgaattaa ggcattagta gaaccaccaa tcgcgtgtaa cacagtcaag
     9061 gcatttgtga gagctttggg tgtgaggatt gctcgtggtg agaacgtttt ttgatctgct
     9121 gtttttgcta actctacggc tctacgtccg gttagttccg acaaacgaaa acgttctgcc
     9181 atgactgctg gcgcagctgc tgttcctggt aagctcagtc ctgctgtttc caccatacaa
     9241 gcgatcgtac tggcagtgcc catcaccata caggttccag ttgttggggc cagcttaccg
     9301 ttgacctcat caatctcctc ttggtcaatc tcgcctgctc taaattttcc ccaatagcgc
     9361 cggcaatctg tacaagcgcc taagcgatct ccctgatgaa gcgtcgttaa catcgggccc
     9421 gtgggtattg aaagcaccgg aatatccaag ctagctgccg ccatcagttg agcgggaatt
     9481 gttttatcgc atccccccac taaaacaact gcatcgacgg gttgtgagcg catcatctct
     9541 tcggtatcga gtgccatcaa gttgcgcaaa tacatactgg ttggaaatgc aaaggactca
     9601 tgaattgaaa tggttggaaa taccattggc ataccacccg cttgcatcac cccacgcttg
     9661 atggcctcaa tcatttgcgg cacattgcca tgacatggat tgaaatcact ataggtattt
     9721 gtgatgccaa taataggtcg ctctaaagcg ctatcgctgt agcccattgc cttaatgaag
     9781 gctttacgaa gaaacagtga gaactctgtg tcgccgtagc gagtcagccc ctttcgaatt
     9841 ccagtttgta ggctctgatt tgcctgtgcc ttcgggtctt tcgctacact catttgatgg
     9901 gttccaaaat gacaaaaata tcgacttaca atgacacatt attccaataa aactgcaaaa
     9961 gagacatgat tccagtcaat agcgtattac aggtcggctt tttccctgaa atcatgcagt
    10021 tagagattga tcgccgtctt gttcccattt ccatgctgga cccaaaggga ccagtccccg
    10081 accgaaatgt aaaggccatc ttaacgcgcc ccagctttgc cattaacccc accctactgg
    10141 accaactgcc cgaaatcaaa atgattgcct gttgcggagt gggatacgac aaccttccac
    10201 tggattactt aaaacaaagg gggatcattg cctccaatac gcccggcgtc ctaaatgatg
    10261 cggtgtgcga attaactatt gggctcctat ttggtctctt gagaaagatt ccggaggctc
    10321 accaatttgt tctccagaaa acttgggcgg ccgagacctt tccattcacc acctccctgg
    10381 ctggaaagaa agtagggatt gtcggtatgg gtcgtatcgg tcaagaactg gctgcgcgtc
    10441 tcttgccatt caaagtggag attgcttatc acggccctag ttcaaaaagc ctaccgtatg
    10501 tattttttcc atccctgatg gatttagcgc gagagagtga tgtcctcata ctgacatgcc
    10561 ctgggggcaa ggcaaccgag aaaattatta atacttcgat cctgaacgca ttgggtccaa
    10621 agggttactt gattaatgtc gcaagaggca ctgtggttaa cgaagatgat ctcatcagcg
    10681 ccttgcaaaa caaagtcatt gcaggcgccg cccttgatgt ttttgaaaac gagccgaatc
    10741 ccaagcctgc tttcttaagt ctttctaacg tactactagc nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnatcg ataatcttga ggcatttttt aatcaccaat cacttccaac ccccatttct
    10861 atttagtcgg agatttttat gtctactcat acctctaccc tatctgctgt tttatctccc
    10921 gtattgaccc cctttaatgc ggacggcagt cccaatggca aaaagttgtt acgtcaatgc
    10981 caatggctcc aaagtaatgg ggttggacaa gctgtatttg ggaccaactc agaggccaac
    11041 tcaatgtctg ctggtcaaaa aatagatgta cttcatgagc ttattggagg aggacttgat
    11101 cctaagaaca tgatgccagg gactggggca tgctctgtag cggatgcatc gacgatgacc
    11161 aaggctgctg tcaaagcagg ttgtgctgga gtattaatgc tcccaccctt ctattacaaa
    11221 gatgtcgcgg atgacggact ctttcagtat tacgcagaga ttattgaagc agtgggtgat
    11281 gcaaagttaa aaatttatgt ttacaacatc cccccagtaa ccaaaattgg tttgagtgtt
    11341 cccctactag agcgcttggt aaaaacgtat cccaacacag tcattgggat taaagatagc
    11401 tcgggagatt ggccctatac cgagtctgtc attaaagcct tgcttgggac tggttttcgg
    11461 gtttatgccg gtagcgaggt atttttactt cgcgccctac gtgcaggcgg ggtaggttgt
    11521 atatcagcaa tggcaaatgt taaccctaag gcaattgcgg atttagcagc tcgctggcag
    11581 gagccgaccg cagaccatgc gcaagaggct ctaacagctc ttcgctcggt ctatgcccaa
    11641 tatccaatga ttccggcgat gaaggccgcc gtagcccatt acagcaaaga cccagaatgg
    11701 tctcgggtca ggccgccgct actggcactt gagcccagtc aacacagcaa gctaatcgcg
    11761 gaacttgaaa aaattggctt ccagatgcct gggctttaat agtcaaaaat gatatagact
    11821 tatacgctaa gcattcatta ttaatagaaa aattaggaga tattgcatga aactttttaa
    11881 agctctcgcc ctttgctctg ccctcgtttg gggcagcgta caagcgcaga atatttctat
    11941 tgcaaccggc ggtactggtg gcgtttacta cccgatgggt ggcggattag catcggttct
    12001 ctccaaaaaa gtaccgggta tgtcagcaac cgcagaagtc acgggtggat cggtggacaa
    12061 cctaaatctt attggtaccg gcaaacccta cgttggcttc tcgatggccg acgctgctaa
    12121 agatgcacaa tcgggtcagg ataaatttac aggtaaaaag gtagatctca gcaccctact
    12181 cgttttgtat cctaatcgga tgcacattgt gaccactggt gctactggta tcaaaacgat
    12241 gcaagacctc aaagggaagc gcgttagcac tggcagcccc ggaagtgcaa ccgaagtgat
    12301 ggcatttcgt gtaatcgagg ctgctggtct cgataaagat aaagacatga gacgtgagcg
    12361 tttaagtgtt gccgagtctg taaatgctgt taaagaccgt aagattgatg cattcttctg
    12421 ggtaggtggt ttaccaactg cggctgttac cgatcttgct aataccccga atactcaaat
    12481 tgtcatgatt gatcactcca aagaagtgga tgcgatgaac aaaaaatatg gcaatctgta
    12541 ctttaaagat gtgatcccaa aaacaaccta caagggtatg gagaaagaca ataatgtgat
    12601 ctcagttgct aatattttag taaccagtcc aaaaatgtct gaaaaggatg cctataacat
    12661 tgtcaaagct gtttttgata ataagattga gttggtccgg actcattatg agtacatcag
    12721 cgtaacattg gaaggtcaaa aaactgcttc cacaccaata ccttttcatc ctggcgcttt
    12781 gaagtacttt aaagaaaaga atgtcaaagt gaattaaggc tagattgttg agaatgctgt
    12841 gtagtaatgg ccgctaatgc ggccattttt taatagagaa taaaaatatg agccaaagtg
    12901 caatcgacag tgcaacccaa gaaaaactag atgccctgat acgacaagaa gaaggtgatt
    12961 ctaatagcta taaaggctta tttgcgatct tcctaacgct tgcagcagtg gcaatgtctt
    13021 tattccatct atatgcagcc tatgccattg ttcccactca ggtattaaga actgtccacg
    13081 ttagctttgt actttttctg gtgttcttaa gctttccact tactgccaag tttaaaaatc
    13141 gactaatgtg gtgggacatt atcttttcct tagcggccat ctacattgcc tattacgcca
    13201 tctctggtgg cgataatttt ggtgatcgta acactgcacc aaactctact gatgtgttca
    13261 ttggcgtggg gcttattctg ttgatattgg aaggcgtgcg ccgaaccaat ggcatgattc
    13321 tggttacggt taccgtcctc tttttgatgt atgcactttt tggtgacctc ctgcctgctc
    13381 catggactca caaaggttac tccgtcgatc gccttgtcgg ttttatgtat atgaccttgg
    13441 agggtattta cggtactgct gtcgatgtct cagctacttt aattatctta tttacgatct
    13501 ttggtgcctt cctacaattt accggggctg gtaagttttt tattgacttc tcctttgcgg
    13561 cgatgggtgg taaatcctct ggggttgggc gaacgattgt tctatcctct ttcttacttg
    13621 gcggcccctc tggctccggt gttgctacaa cagttactgt aggctccgtt gccgctccca
    13681 tgttagataa agtaggctat gagaaaaacg ctgcgggcgg tcttttagcg gccggtggtt
    13741 tgggtgcgat tatttctccg cccgtattgg gagcagccgc tttcttgatt gctgactttc
    13801 tccggatctc ctatctagac gtcttactaa tgtcgtgcat tccaaccatt ttgttttatc
    13861 tgggtctctt tacgatggtg gaaattgatg tacgtaaata cgggatgaag gcaattcatt
    13921 ttgagaccgt ggagagcgct tggtcactta gtaaaaaata ttggttccac ttcttctcac
    13981 tcatttcaat tgttgtcttc atgatgatgg gcttctcacc catcatgtca gtattttggg
    14041 caaccgttgt ttctgcactg acaagtatgt tgcgccgtga taccgctatt ctttcttacg
    14101 attggcttac tggtaagcag cctttcttaa aaggtctcta cgaatctaac ttaacgaagg
    14161 cgctttcttc cggatctact ggtgtgcttg ccattgcagc gacctgcgct ggcgccggtc
    14221 tcattgttgg tactgttacg ctgactggct tagggcttaa atttagctcg atcgtcattg
    14281 catatgcagg cggctcatta cttttaacgg ctatctttac cgccctgatt gtttggattg
    14341 tgggtctggc tgtacctgtc accgcctctt acattatttg tgcagtgatt gcggctccag
    14401 cactaattaa tcttggggtg cccgcctttg ccgcacacat gtttatcttt tactacgctg
    14461 ttttatctga ggtgtctccg ccaacagcac tgtcaccctt tgcggcagcg gcaatctgta
    14521 agggcaatcc ctacaaaaca acgttacaaa gctggaaata cgttgcgcca gccatattgg
    14581 ttccatttat gttcacccta gataaatccg gtgtgagctt gctcctcatg ggctcaacca
    14641 cagcattagc acaagcgaat tggattgaga ttgcttggtc aacctttaca gcaattgtgg
    14701 gtattgtttg ccttgctggt ggtttacaag gatggtttat tgagaaaaca aatattgtgg
    14761 aacgtctctt aatgatcgta tcgggtgtcg ctcttgccta tacatcaccc acgagtgaca
    14821 tgattggctt tgccggcttt gcgattgtct tggtcattca gttctttaag cacttcaaga
    14881 caagacctaa acaagccagc taactccctt cattaaaaaa atagccagta ttgaactggc
    14941 tatttttctt gtgctacggg actactattt acttaccaat cttgctccaa gcagatttgc
    15001 cagcatattt tttcacagct ccttcatcaa actcaaatcc caaaccgggg tttttatgca
    15061 agatcagatc accattcttg aacgttaatt gcttgttcac taaacgcctg aaatttaaga
    15121 cctggttgtc tgggaagaac tccacaaagc gtgcattcgg agttgcagca acgagtggcg
    15181 cgtgcagatc atgaaaccaa tgaggacata cagtgacccc tttggagtcg gcatacgctg
    15241 caatccgcct aaactcactg ataccaccgc ataccgccgc atcggcttgc aagatgccag
    15301 caccgcctgc atctactagt tctctaaacc gccaccgacc aacttcaatt tctccggtag
    15361 caatcgtgat cgatgtgctt ttagctaagc gagcatgcaa atcgatggca tcaggagaga
    15421 atggctcttc taaccaatag ggattgtatt tctcaaaccg acgaacatat tccatcgcag
    15481 ttggaagatc acgccaagcg ttgttggcat caagggttag taatatatcc tcaccgatag
    15541 cgctgcgtgc ggctttcact cgcgcctctt cttctcgtgg cgataggcgc ccaactttca
    15601 ttttgaccgc cttgaaacct agttttacaa aagactccat ctctttgccc aatttggcgg
    15661 gggttttgcc ttccagatag tagcccccgc ttgcgtaggc tggaacgcgg tcgtcgacga
    15721 cgcagcccat aaactgatgc aagggtaggc ccacagagcg tgcattgaga tcccaaatag
    15781 cagtatccaa aatcgagata cctcgcatga ccgaaccagc tcgtccctgg agaatggatt
    15841 cagcatacat atcgttccag agaccttcac tacgatgact attctgccca attagtttgg
    15901 gtgctaagag ctgctcaacc gcagtctttg caatatcacc tgctgcagag ccaacgtagc
    15961 aaaagccaat gccttcaaca ccctctcgac ttctaacttt aacaatgcaa tagtgtcgct
    16021 cgctgacagt tcgggtcgaa aaggaggtta ctacatctaa agggataggc actgttgcga
    16081 cctgaacaga ttcaataatc ggcatttttt tctcctactt aatcatcaat caaagaaaaa
    16141 tgattgtagc gattattttc ctgtagcttc agcaagacaa cgtttcataa aagtatcttt
    16201 tgcagcatca attaatttac gctgacgggc actgtgctcg cactcagact gtgcttgtgc
    16261 catgcaatct tgataggata ggggtttgga gttagggttt gatggatttt ggcttccaga
    16321 cttctgagca caagtcttac cccacgcctc cgataagggg gaaaaagaaa gaagggctgc
    16381 agtcattaag gacattttca tcatcgctac ctcctggtga tggactagac atcataccca
    16441 atttacatct agaaaaatgg tgcagcaccg cataatagtt atccctaaat tttgggatat
    16501 tggataatca gtctgtgcat caaaaaaata tcctcaagtc ccaaagacca catattcttt
    16561 ttaacctagt gggggtgttt ttttctatct tcgtcgtcgt gcccctcact agctctgtag
    16621 tgagcgctca aaatattccg gcgagtaaag ctgttcaagc gaaggcggtt agcaaacaaa
    16681 aagggattca ggtcaatcaa cgtcaggcaa ataaaacggt taaagataca agcgcttctt
    16741 tgaccgaccc tggaactgaa gaatatagtc aatttgatat tgctcccgac tttagggtta
    16801 gcagagggtg catgcaacgt ggttttcgag atagccgact acctgaaaga attggtatcg
    16861 aagaccctga atttttagat tactttagaa atcagacggc tcgttttttt aacaccatcc
    16921 aaggttcatg cattccctat gttgcagcat ttggtaaacg caatcggcta gagtcactca
    16981 gtattatgaa tgggccgact cgtaacgaaa aatcgcaaat tcttacattc agtatgtcgt
    17041 cttttggggg atatcgcgtt caggaagaaa acctagtcga ccaaatgcaa ttctggaccg
    17101 aagttctgat tccacttcaa gaagtcctct atgatcccca acgtcttgga gacaaattgc
    17161 cggtagaact tgtttgggaa ttaaactcta ttgtgaagca gatttatcca gatgattctg
    17221 gatcgattga gagcacaccc atgcaggttc gcttaattgt tgattttgga acccgcgacc
    17281 gttgggcaca aatttgggca gtagaaatcc tcaataccaa aacgaatcaa attgttgcag
    17341 atgcattttg gctcaatcgc cctgaaatcc ccggctcatt ttttacttcg agtggtgagt
    17401 cgatcgaacg aactttctgg accaatccac tcagttaccg acgaatttcg agaggggttg
    17461 ggcaagtttt gattcggtcc agaccaaatc cgaataatcc acaagcacaa aataaagacc
    17521 ggcgctatcg aactcatatg ggcattgact atgcagcccc aacgggcacg ccagtcttta
    17581 gcgtcgcaga cggtaaagtg gcctacattg gatttaacgg tgcctatgga aagctcatca
    17641 tcattgatca ccctggcaac taccgaacct actatgccca tttaagcaac tacaacaatg
    17701 agttgatggt gggtaacgat attcggcgag ggatggagat tggctatgta ggctcaaccg
    17761 gtcggtcaac cggtccccac ctgcattacg agttacgtaa gaatggaatt tacgttgacc
    17821 cctataaccc aaagatacag ttagatttat ggacaatgcg gccaagtgat agtggtcaat
    17881 ttactaaaca gcttttattg cttggtaaca ttaatccaga atgaagttgc catgttaaaa
    17941 actcttcgcc ttgaatccgt ctcctgcatc cgcggaggta agtctttatt tactaatctt
    18001 gatctagaaa taaagcctgg ctccattttg cgcatctcag gtgataacgg ctcgggtaag
    18061 tccagcctct tgcgtattct gtgcggactt ctaccccctc aagcaggaaa ggtagtttgg
    18121 ggtgaccaat tgatccatga agatcgcgat caatttcata gcgaactaat ttatctgggt
    18181 catattcctg ctttaaaagc agatttcact gcgattgaga acttgatgag tcttgcgctc
    18241 cttagtggtc aatcgattac agcggaagag gccttgagcg ccctcaccgc ggcagattta
    18301 gcagatcaag cccatcgtgc tattcgcacc ctttcacaag gtcaaaagca gcgtatcgca
    18361 cttgctcgcc tccgcttacc caacccgaag cctctctgga ttttggatga gccatttaat
    18421 gccttggatc aacaatctaa tcaactgctt caatctttat tgcttgccca tgtgagacag
    18481 aatggaatgg tggcgattag cagtcatcaa gctctgagta ttgatgatga cccgcaagta
    18541 atgaggctcg tactatgagt cattctgcaa cgagtacctc tatctttatc ggcattatca
    18601 aacgtgatct gcaacttgcc ctgcgacgta agagtgacag tctttcggca ttgatatttt
    18661 ttgcagttgt tgccagcttg tttcctctcg ggattggtcc tgaaacaaaa ctcttaatgc
    18721 aaattgcccc cggtatgctg tggatcgctg cactattagc agccatgctg tctttgcata
    18781 agatgtttac tgaggatttt atggacggat ccttagatca aattctattg acaaaactac
    18841 cactatctct tgtggtcttg gcaaagacca tcgcacattg gctgagtagt ggtgtcttgt
    18901 tagcgctgat ctcaccaatt ctggctttgc aatttaacct gccgtttgat agcgttgttg
    18961 tactcttttt atctcttctc ttaggcaccc ccctactaag cctgattggc tcgattggcg
    19021 cgggtttaac cctagctagc cggggcggcg gtaccctctt gtccttaatt attcttccgc
    19081 tggtagcgcc agtgctcatt tttggagcag gcgctgtaga ggcctatcaa gcaggcttag
    19141 gattaactgc tcatttttca ctattgggtg caatgctggc aattgcttta tttctggcac
    19201 ccatcgccac cgctgctagt ttacgaattg cactggaata agaccaatca atacaatgat
    19261 tcgacttaca atacttgcca agaccaccat taagaacttg tgacctattt tgtctattcg
    19321 tgataacaac tggtttaagt atgccccgcc gcagcgcttt tatgagctct caggaaagnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnntc ctatcggatt atttttattc atgtaccggc tgcttggatg tccatgctga
    19501 tttatgttgt gatggcattt tgggctgcaa ttggtctcat ttttaatgcc cgcttagcaa
    19561 gcatgttggc tttatcgctc gctcccactg gggcaatcat gacctttatt gcactctgga
    19621 caggggcggt ctgggggaaa cccacatggg gcacatggtg ggtctgggat gcgcgcctga
    19681 cctccgagct tgttttattg ttcttatatt ttggtttctt atctctgcac gcctcaatcg
    19741 atgatgtgcg caaggcagat cgtagcgcag ccctattagc tattgttggg gtggtgaacg
    19801 tgcctattat ttacttctcg gtgaagtggt ggaacaccct tcaccaagga gcctccatta
    19861 gcctcaccaa ggcaccaacc atggcaaccc aaatgctaac cggtatgtta attatggttt
    19921 ttgcgttttg gatgtactcc attgcagtag ccctataccg ctgccgaact ctgatcttag
    19981 agcgtgatcg caatgcccaa tgggttaagg aggctatgta atgttttact ggtcaaactt
    20041 tgccgatttc ttgaatatgg ggggttatgg cctatatgtc tgggggtctt atctagtcac
    20101 cgctttagtc ctaatcattg aggttttaag gctaagaagc cgaaatcagg ccctcagaaa
    20161 aaccctaatc tctgaattcc acgagtcttc taaaaattag gcaaaatacg aggttatgaa
    20221 accccgtaca aaacgcggtc ttgcgatcgc tggcggccta gcctctttag ggattgcaac
    20281 ctatctagtc ttgcaaactt ttcaaagtaa tttggttttc ttttttagcc cctcccaggt
    20341 ggtggcaaat gaagctccaa aaaataaacc ctttcgcatt ggcgggcttg ttaaggaagg
    20401 aagtattcag cgtgacccgg gtggcctcaa agtgtctttt attgtgacgg atacggccca
    20461 tgatgttcag gttcaatacg agggacttct gccagatctt tttaaagaag gcaaaggcgt
    20521 tgtcgcacaa ggtcagcttg gtagcaatgg catctttgcc gccaaacagg ttcttgccaa
    20581 acatgatgaa aactatatgc ctccagaagc tgctgaagct ctcgaaaaag cgaaatccaa
    20641 acaaccacaa acagcgccca ctcttaaaga atctgcggga ggcaaaaatt gattgctgaa
    20701 attggtcaat actctttagt gctggctttg tttaccgcaa tcatcctggc ggtatttcca
    20761 atccttggcg cacaaaccaa tcgtccgcaa ctcaatgcca ttgccagacc cgcttcatgg
    20821 gggctttttg tttgggtggc catctctttt atttgcttaa ctattacatt tattgagaat
    20881 gatttcagtg ttctctatgt ggcacaaaac tcaaactccc aacttcctct catttaccga
    20941 atctgtgcag tttggggcgg ccatgaggga tcgattctcc tatgggctct tatgctggct
    21001 ggttggacct tagccgttgc ggtattttct aaacacttac ccgacaaaat ggttgcacgg
    21061 attttaggga ttctgggtat tttgagtatt ggtgtgttgt tatttacgct cttaacctcc
    21121 aacccttttg atcgtttatt tccttcagcg cctgaaggta gagatctcaa tcctctttta
    21181 caagaccccg ggatgattat tcatccnnnn nnnnnnnnnn nnnnnnnnnn nctatcgggt
    21241 cgcttagatg ctgcatgggc aaggtggtcg cgcccatggg taacaaccgc atggtgcttt
    21301 ctgaccattg gtattgcctt aggtagcgca tgggcatact acgaactggg ctggggtgga
    21361 tggtggttct gggatcccgt ggaaaacgca tccttcatgc cttggttggt gggcaccgct
    21421 ctgattcatt cattggcggt taccgagaaa cgtggcggtt tcaagatgtg gaccgccctc
    21481 ttagcgattc ttgcgttctc actctcctta ctaggtacct tcctagttcg ctctggcgtc
    21541 attacctctg tgcatgcttt tgctaccgat ccagagcgcg gtatctttat tttgggtttc
    21601 ctcgctttcg tggtcggcgg ctcactttta ctctttgcgt ggcgtgcccc aaaagccaac
    21661 atcggtggta attttgagac tgtgtcgcgt gagggcatgc tcctcgtcaa taacgttttg
    21721 ttgttagttg ctgctgctgc agttttatta ggaactctgt atccattaat cttagatgcg
    21781 cttaatctag gcaagatctc agttggcgag ccctattttg aggcggtatt tgttcctcta
    21841 atgacccctg ctctcttttt aatgggcgtt gggccaattg cacgctggcg acagtctccg
    21901 ccgattgaac tagttgtgaa gttgaaatgg gcttttgctg tgagcgctat aacggctgtc
    21961 attgcaccaa tcatgctgcg gtcgtggacc ccaatgattg gctttggact cttggtctct
    22021 gcctggatta ttagctctgg ggttacacaa ttggtggatc gtgcgcgcat taatcctgat
    22081 cgatcctggt ggcagaattt acggattcag cctgctggat attacggcat gctaatggcg
    22141 cattttggag ttgccgtttt tattattggt gtcactgcag tacgtggctt tgaaaccgaa
    22201 caagatgtgc ggatgcaagt gggtggcgtc tctcaatcag gtggctatga tttccaatta
    22261 gtttccttaa aaaccattgc tggtcccaat tacgctgcta tacagggtca ttttgatgtg
    22321 agccgaaatg gtaagccggt gacaagtcta tatccagaaa aacggattta taccgctagc
    22381 cagatgccga tgaccgaagc ggccattgac tctggcctaa cacgagacct atatgtttca
    22441 ttgggtgagc ctattaatga caatgaatgg agtgtgcgta tttatcacaa accctttgtt
    22501 gattggatct ggggtggttg ttttctaatg gccttaggtg gcttcttggc tattacagat
    22561 cgtcggtatc gcaagaaaga ggcttgagat gaaacgctat ctcattccgt tgttaatctt
    22621 tgttgtgttg ctagtatttt tagtcattgg acttcagctc aagccccgcg aggtaccatc
    22681 cccattcatt ggtaagccag caccagtatt ttctctgcca gtattaggaa aagagcaaag
    22741 cgccttctca ccagagcaaa tgaaaggcaa agtttggatc ctcaatgtgt gggcctcttg
    22801 gtgcgtagcc tgccgcgagg agcacccttt actgcttgat tttgcaaaat cacaaaatac
    22861 ccccttgatt ggcttagatt acaaagataa acccgaggcc gccaaggcat ggctaaaaca
    22921 atacggcgat ccttatgaac tctctatttc tgatcttacg ggtcaagtgg gtattgatta
    22981 tggcgtgtat ggagtcccag aaacatttgt gatcgatcgt tctggaatta ttcgatataa
    23041 acatattggc ccccttactc aggaggcgat cagccagaag attattccct tgattgcaaa
    23101 gttacagcaa tgaagttcat taggcctccc attcttgcaa ccctctcgct actcctgata
    23161 gtttgtgctg atgtaggcgc ccaagttagt gcgagcgcta atagctcagc aaagatagca
    23221 actccggttg ccagcaatcc cgaattagag aaaagagtat tagagctctc tcatgagcta
    23281 cgatgccttg tctgccaaaa ccaaaccatt gcagactcta atgcagattt agcagtggat
    23341 ttgaaaaatc aagtacgcaa acaactaagt gagggtcgct ctaaagaaga aatccttaaa
    23401 tacatgactg atcgttatgg tgactttgtt ttatataagc cgccttttaa tgctgcaacg
    23461 atcatgctgt gggtaggccc gtttttacta atgttgtttg gtctattttt cttagtccgt
    23521 caaattaaac aacgccggca agacctttta ggtgaatcct tctctcccca agacattgaa
    23581 cgtgcccgac aactattaga tcgtaagaca gaaaactagt aatgaccatt ttcatcatcc
    23641 ttgccatcat catgactgta cttgcggtct tgattattgt gattccattt cgcagaaagc
    23701 aattagacct tcaaaaaatt agccttgagc aagatgagaa tattgccatc ctacagaatc
    23761 agttaagtca gtttgaattg gattttaagg aagggcgaat taatcaagag caattagatg
    23821 aatctcgtct tgatattgag aaacgccttt tacaagaaga gcgtgctatt gctgcagatc
    23881 agtttaatcc ggagaaagtc actcaaaaga ctggtaagaa atggcccaca atttggattg
    23941 cgacaggttt accactatgc gccattcttc tttatctcct agttggctca ccactcgccc
    24001 tctatcttcc tgaagcaaat caagggcaac cgcagctgac ccaaaaagat attgaaggaa
    24061 tggtcgagcg ccttgctgat aagctcaaga aagagcccaa taatgccgaa ggttggcaaa
    24121 tgttaggtcg gtcttatgct gccctacaga aaatgcctga ggctcttgct gcttataaaa
    24181 aagcattaga gctgagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgc gcctaatcca acaagcttta
    24301 aaaattgagc cgaataatct aaaagccctt gctttaggag gcaccgctta ttttgagcta
    24361 ggtgacttta agaaggctga ggaatactgg actaaggcaa agaacttggt tcctgccgat
    24421 agcgagtttg ctcgcggtat gacagaaaat attgcctccg caaaggcaga ggcaatgaat
    24481 cgttctaaaa aataaatggg gttaacccaa gaccaaaacg ggcaaattgc tgtgaacgat
    24541 cactgcatgc gtttcgcttc ctagcaaaat tccttttaag cctgtgtttt tgttcgatgc
    24601 catcacaatg acatcccctt tggctttttt agccgtctca ataatgccct caaataaatt
    24661 gacattgaaa acatggcgca tgtccacagt taagcccttg cctatttttt ccatcgattt
    24721 cttgaggatt ttcttagcaa atgattcgca ggctttttta tgctgagcat cagagaaagg
    24781 tacccccatc gtgctatccg catatgcaat gggtggcagt gggtcagata catacaccaa
    24841 ggtgatctta gctccacccg atttagccaa ttgtgcaact ttttttaagg atttaaaact
    24901 aagatcgcta ccatcaactg gaacaattaa attcttaaac attgaacacc ccttattagt
    24961 agttttgagg atttatactc ataagactat gttaagtctt attaccccga aaagggaagg
    25021 gttctaacca aaatgctcaa gtatttagtc ccatatttgt ccttcttaat tggtctctta
    25081 gtgatagatc tcatttggct attaggcatt gccaaaggtc tttatcgctc cgaaatgggt
    25141 tctctgatga ccgcaaatcc taatttagtt gctgccttag gcttttatct tctttatagt
    25201 ctgggagcaa ccatctttgc tgtttaccct gctctccaaa agcaagcttg gcttgatgcc
    25261 ttgatatatg gcgccctctt tgggttcttt tgctacatga cctataactt aacaaatcta
    25321 gcagtgattc gtgactttcc cagcaagtta gctttaatag atatcgtttg gggaagtatt
    25381 ttgaccgctt catgctctgt tcttgcttat tacgtgagtc aatactcaga aaaatggttt
    25441 agaggctaac acaaggggac ttaatgcacc caacccttac ggcgctcata gatcacaaat
    25501 agcactcccc aaatcacaat cgatgcgtat gcacagatcc atgaaatccg atccgtttgt
    25561 gagccgctta ggtcaagaac gaagccaaaa atagcggggc caagaagtcc gccgccaaag
    25621 cccatcagag aatgcaaacc cattgccgca cctttaatct ttgggtcagc actagtaacc
    25681 aagccagccg tcaaggtcgc tgaatcggcc ataataaaaa tggcatgtcc aatagccaaa
    25741 gccaaaatga gccaactggc gtgacccatc gagtaggcta gagcaactcc aaagatagcg
    25801 ctcgtgatca tcacaatgca gatccacttt tgacgaccta ttttgagagc taactcatta
    25861 cctaatatag atgctggcac cccaaagaaa ttaataacgc cggctagagt agtcgctgct
    25921 aagaaaaatt gctcaccaga taactgagta ctcaaaatga agaatgccac taaccagcta
    25981 cgcgatgcga acagctctag agaatgaaag gtgtagccta gaataaagcc agatgcattg
    26041 cgatcttgta atacctgctg ccactttgcc actggaaaaa tatcgcgcca gttaattact
    26101 atcctatcgt gccaatgaac atcatgcatc ggccgaacgc agaaataaac gatcaataaa
    26161 gaaatcgtag ggcccagtgc aagccaccga taaacatgat gccaatctcc taaattaaga
    26221 acccagccag atacaacata cgataaaccg gtaccaacac caaagaacgc ggtgtaaaaa
    26281 gagatgtgac gggtgatctc gccctgcgtc aaacgatcgg ataagatctt aagtcctggc
    26341 atataagtag cggcaagtcc tgcgccatta attgccatgc acaggcaagc gctatagaaa
    26401 tcgtttgcca gaaagcccat tcccaataaa ccagaaattg ctaatgtact gccaaataaa
    26461 taaatacggc gcgcatccat ttgatcggtt agcgcagttg caaaaggtac cagaagaaca
    26521 taaccaacaa aaaaggagct cgcgattaag ccagattcaa aattactaag accccactca
    26581 ttctgtaagg tggtcaaggt aactgcatac gccgcaaatc ctaataaagc accaatctgt
    26641 gccaataaca ttagtggcgt attacctttc aaaatactca tgtattggtc ttattgatct
    26701 tggccgtggg gtggctcttg aaagccgttg cgacgaaagg taataactaa ggtatcgcga
    26761 tagccgtgtg cagcagttgg ctgaattggg gtcgtttcat gaatcatctt ggcatcattt
    26821 agcagcaata cactccatga gttttgcaag gtaaagcgca gtcctattga gccctgagac
    26881 tcaaaaatac gggtctcccc accctttacc ccaacccgat caactaagat cactgccaca
    26941 aaatcaactc cgtctcgatg cgcaccctct ggagtgggcc gaccaattcc atcactcgta
    27001 tcgatacgga atggatgcac ttctacaaac catgcaggtg atggagcgat tgcggatagc
    27061 aattgagcta tcttgataat tagttgttgc cagtgttgat tctgagcaag ttcaggctgc
    27121 aatggctcaa accaacgctc aatgccgcca tgcaaggcgt tgtaatccaa tgattgccaa
    27181 tgcgctcgat gtggtgccat ttccaggcta gagtcacgta cgatatagct agcatgccta
    27241 cgataacgat agcgcccacc atcctttaaa taaggatcac ggggtaaatg attccaatat
    27301 gacttaaaca tatttaactg atctagggat aaagaaacct gtttagccaa gttatcagga
    27361 gacaaaacaa cataaccacg ctctcctaaa aaagagtgtg cttgctcaat tggtgtaaag
    27421 ggaggaagta acatcaaata attaaacaaa tagttatggt tggttaatct gaatttagcc
    27481 tatttaccca tattcaagtt aagaaaaaat ctcatgaaat aagactaact agaaagtgag
    27541 ctgataaaag gttcatggct ttatcattgt atttttaaca attatgccct gattgatcaa
    27601 attactatga actcaaactc tttacgtgtt gcccttatta ctggtgctgg aactggaata
    27661 ggaaaagctg ctgccaaagc ccttttagca gacggcttct gcattgtgct gacaggtcgc
    27721 aagctagaac gccttacgaa ggccattgag gagattgggg gtaatgacaa gaactgtcta
    27781 gcggtctctt gtgatattgg aaagcccgag gaggttaaat cgctatttac cgctctcaag
    27841 aataagttcg gtcggattga cgtgctcttt aataatgctg gtactggtgc tcccgctatt
    27901 ccgatggatg aacttacgta cgatcagtgg atgaatgtgg tcaatagtaa tttatgcggt
    27961 gcgttcttgt gctcacaaga ggccatgcgc atgatgaaag cacaatctcc acaaggaggt
    28021 cgcataataa ataatggctc catctctgct catgcacccc gccccaacac agctccctat
    28081 acgtcaacca agcatgcgat tacaggtctg accaaatcta tagcattaga tggtcgtccc
    28141 tttaatatta attgtgggca aattgatatt ggtaatgcag ctacggagat gaccgagcga
    28201 atggccaatg gcattatgca agccgatgg
//
LOCUS       DDPQ01000044            5355 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_17771,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000044 DDPQ01000000
VERSION     DDPQ01000044.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_17771"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    306..354
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1377 a         1302 c         1234 g         1393 t
ORIGIN      
        1 tctgctgcgt gatccagtaa ctgtcttaat gaaattaatg ccattctttt ttcctctatc
       61 ccaatctcta gttatacgag ttccacaatg cgctcaaccg tcacacccaa tgctttgtat
      121 acagcagcgg ctggagcaga ctcaccgaaa cgattaatac ccacaacggc accatctaaa
      181 ccaacatact tccaccacag gtcggggtga cccgcttcga tcgcaatccg tttgatgccg
      241 tttggtaaca ccttttcttg ccagctttta tcttggcgat caaatagaga agtgcatggc
      301 atggannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtcgcg
      361 attaagaccg catccggttt gccctcacaa tccgacaaga catagccccc tcgcatcaca
      421 tcggcttcac tgatgttctt actgagttgt ggcaagtttt gtcgagacaa gaacaaagca
      481 ctcggcccat cggtgcgctc aatcgcagca gtccaagcaa tcgcagtttc aaagccatcg
      541 caaggacgcc acaaatcgag atgaggaatg agacgcaaac tcgccgcatg ctcaatcggt
      601 tgatgggtgg ggccatcttc acccagacca atcgagtcat gggttaaaac ataaataacg
      661 cgttgcttca tgagagcact catgcgaatc gcattgcgcg catagtcgga gaacaccgca
      721 aaggtgccgc catagggaat aaatccttta tgcaatgcca ccccgttcat gatggcgctc
      781 atgccaaact cacgcactcc ataagaaatg tagtttgcag cttgatcggt cttgtgatgc
      841 caggtaatgc tggtcttcgc agccgtgagg tttgaaccgg taaggtctgc tgacccacca
      901 aggagttctg gtaatgcggg caccaggatg tctaaagttt gttgagagga tttgcgactg
      961 gcggttggtg cttctattgc cttcatgctg gcaaacaatt gagtcttagt ggatgaccaa
     1021 gtcgctggta gcttttcagt agcgcaacgc atcagctctt tggctagctc tgggaatgcc
     1081 gcttgatact ttgctaattg ctcattccat tgggcctgtt ttgctttacc gctctctact
     1141 gcatcccaag cagtcttaat cggcgcaggg atctcaaagg gaccatactt ccaacccaaa
     1201 gcttcgcggg ttgcagcagc ctctttctca cctaagggtg ctccatgaac atcatgcgta
     1261 cctgccatat tcggtgcacc ccaaccaatg gtggttttac agatgatcag agtcggtttg
     1321 gcggtttcag attgagcggc ggtcgtggcg gcatttactg cttgagcatc gtgcccatcg
     1381 atcggaccaa tgacattcca gccataggct ttaaaccgac ttgccgtatc gtctttgaac
     1441 caaccatcga catgaccatc aatcgagatg ccgttgtcat cgtagaagca aattaatttg
     1501 ttcagccccc aaacgcccgc gagagaacaa acctcatgac taataccctc catcaagcaa
     1561 ccatcgccta agaaggtata ggtatggtga tcgatgatcg tatgacctgg acgattaaag
     1621 cgcttagcaa gaagcgattc ggcaagagcc atgcctactg cgtttgctag gccctgacct
     1681 aatggtccgg tggtggtctc gacccctggc gtaatgtcca cctcggggtg tccggcagta
     1741 atgctatgca actgcctaaa gtttttaata tcctcaatcg agactggata accggtcaga
     1801 tgcaacaggg cgtagatcaa catcgaacca tggccattag aaagtacaaa gcgatcccga
     1861 ttgatccaat gtggatctgc tggattatgg tttaaatgat ggcgccagag cacctcggca
     1921 atatccgcca tgcccatcgg agctccgggg tgacccgatt ttgatttctc taccgcatcg
     1981 atggctaaga aacgaatcgc attggctaac tctctactag taatttgtgc tgtgctcata
     2041 tccaagatta atttgtacta tcgtgttcac ggtgatactg ctcaacccgc tcgacttcat
     2101 tgcgcgatcc caaaacgaca ggaacacgtt gatgaatact ttctggtttt agatccaaga
     2161 tacgttgacg tcctgtggat cccaaaccgc ctgcctgctc caccacgaaa ctcataggat
     2221 tggcttcata catcaggcga agacgacctg gcttacttaa atcttttgta tctttggggt
     2281 acatgaatat gccaccgcgc attaggatgc gatgcacatc cgcaaccata ctagcaatcc
     2341 aacgcatatt gaagtcacgg ccccgcacat cggtcttgcc ctttttgcat tcactaatgt
     2401 agcgcgcaac gggtgcttcc caaaaacgct cattgctcgc attaattgca aactcactgg
     2461 tgtctgcggg tatctggata ttgggatggg tcaggatgaa ctcacctgtg cctgcatcca
     2521 aggtaaatcc atgagtacct ctgccgatac ttaatacgag catagtggat ggtccatata
     2581 ccgcatagcc agcagcaatt tgttttctgc ccgactgtaa ataatccgct aactctgggc
     2641 tgcgatcttt aggggcatgc tgaattgaaa agatcgttcc caccgagacg ttgacatcaa
     2701 tattagaaga gccatctaag ggatcaaaca aacacagaaa aggcccacca tttttattag
     2761 caatcggctc atcattttct tcagaggcaa tgccagcgac caagttcccc gatcgaaagg
     2821 tttgaatgag gatctcatcg gagagaacat cgagtttctt ttgggtctcg ccctgaacat
     2881 tttgactggc taaattaccc aagaccccag ctaatgcacc ttgcgaggtc aatatggcaa
     2941 tttctttgac ggcggtggca atatcgagga tgagttttcc taaatcccga tgaatcgact
     3001 gatctgcgtc cagctgctgg cttaaatggt cagaaagtct ggttctccct gcttccatgg
     3061 tgggggtctt taggcttatt aatgaaacct ctattttctc agatatttaa acttggccac
     3121 cctttttgga caaaaggtat tctagtaagc tcttaaggca tatatccact agaaacttac
     3181 ccatgaaaac atacaaaatt gcatccattc ctggtgatgg gattggtaaa gaagtaatcc
     3241 cagagggtga gaaagtcctt catgcgatta gcaagcgctt tccagaaatt acctttcaat
     3301 ttgatcgctt tgattggggc ggagactact atcgcaagac tggaaaaatg atgccggatg
     3361 atggcttgga gccccttcgc tcaaaggatg ccatcttatt cggttccgct ggtgatccac
     3421 agatacccga tcacatcacc ttatggggct tacgtctcaa aatctgccaa ggatttgatc
     3481 aatacgctaa tgtgcgtccc acacggatac tgcccggtat ccagacaccc ctgcgaaatt
     3541 gcaaacccga tcaattggat tgggtgattg tgcgggagaa ctccgaaggt gagtactctg
     3601 gattgggtgg ccgagcgcac caaggtcatc ccattgaagt ggcctctgat atgagcatca
     3661 tgacgcgcgt cggtgttgag cgtatccagc gttttgcatt tgagctcgca cgttcgcgtc
     3721 ctaaaaaaca tctaacggtt attactaaat caaatgcgca acgtcatgcc atggtcatgt
     3781 gggatgagat cgcggttcaa gtttctagag atttcccaga tgtgggctgg gataaggaat
     3841 tggtcgacgc cgctacagcc cgaatggtca accggcctga gtctctggat actattgttg
     3901 cgaccaattt acatgctgat gtacttagcg acctagcagc cgccttagca ggtagcttgg
     3961 ggatagcccc aacagggaat atcgacccgc aaagacgcta cccctccatg tttgaaccaa
     4021 ttcatggctc tgcctttgac atcatggggc aaggtctagc aaaccccatt ggtacctttt
     4081 ggtcaattgt gatgatgcta gaacatctgg gcgagcccac cgccgccaag ctcttgatga
     4141 gcgccattga atccgttaca gtcaatccta cccttcacac tcgcgactta ggcggtaaag
     4201 ccatgatgaa agatgttact gctgctgtag ttgcggctat ttctataaat aaataataaa
     4261 cctagggtag atccgaagtt cttatttcat cgttctagtg aaaactgatt agtactagga
     4321 gggcttatga agaaacttat ccgctttatt ctgaatgctg tatcgatggg gctgatgctt
     4381 ggccttggta cttcctttgc acagccagtt attgataagc aaatacagta catcattcca
     4441 tttcctgccg ctggtgaatc cgacatcgtc gctcgtttac aagctgatat ctctgccaaa
     4501 aaatttaatc agcccatgat tgtggtgaac cgtgcgggag cgggtggcgc gctggtatgg
     4561 agccaactta acacctatcc gaatgatggc agcaatgtga ttggggtcaa tattccacat
     4621 gtgatcttgc aacccctgca agaaggtatc caatacaaga ctgcagatat taatgccatt
     4681 tattactatc actttacgcc agatgcattg atggtatctg ctgatagtcc gtataaaaca
     4741 taccaagact ttattgccgc agccaagaaa agtcctggta agttaacgct agcgggttct
     4801 gcgcaatttt cggctaatca catggctaca gaaagactga ataaattggc tggaattaag
     4861 gttgaatacg ttccgtttaa gggaaccggt gatttgattt cttcgctcat tggcatgcac
     4921 gtcagtggcg ccatgggata tttaccgctt gccattcaac aaaaaggaaa ggtgcggacc
     4981 cttgcgattg ccaccgagaa acgtcaccct gccctacccg atgtgcctac ttttaaagag
     5041 ctggggctca attgggtcga tggtgcctat cgtggagttg ccatgcctaa atccacgccc
     5101 atcgctcttc aagaaaaaat gtcagacttt attggtaagt taaatgccga tgtggatacc
     5161 aaaaaacgtc ttgaggatat ggggtttgtc ctggtggata ttccacttaa acaaattccg
     5221 gcatttatga aagagaaaac tccgctggca atggaagatg ctaaaaacgc tgggatgatt
     5281 aagtaagcga tctaaataag cgaacaaagt gagcatattg tgcttacttt ggctttttgg
     5341 ccttgtactt tgcaa
//
LOCUS       DDPQ01000045            3151 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_29491,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000045 DDPQ01000000
VERSION     DDPQ01000045.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_29491"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    190..295
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    465..539
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    934..945
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          807 a          626 c          704 g          819 t
ORIGIN      
        1 agcatagccc gtttattggt ttgaggttca ctgctatttt gattgcgttg acattgatac
       61 atttttagaa acatgtcctc agtgagccca aattgctccc aatcaaacat gagattgatt
      121 tgttcctcct tcattgcctc tcgtgttgga tccttgatac ccagcatact tttttctaga
      181 agcgtatcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagggc
      301 agctgtaaat gatcgaatct gatcaagctt tgcttgcagc actgcaagat cggcatcgct
      361 ctcaaaatca gcatcctgca agaacatcgt attccttatt cattgacccg aataaaacgc
      421 ccatcttcca tttctgtctt tgggcggatc caatagctgc cattnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
      541 gaaatttggg tgaagtgctc atcagaaact tatatggata gtctataaaa tctccacttt
      601 acattgatct ttataaaggc tggaaatgaa cttgctgacc cccattacct taggtgcagt
      661 ttctcttgag aaccgagttt tgatggcgcc actgacgcgc atgcgggcag ataatgaggg
      721 ggtacctagc cccatggcaa ttgagtacta tgcacaacgt gcctcagccg gtctgattat
      781 cagtgaagca acgcaaatct ccgagttagg caagggctat cccgcaaccc caggaatata
      841 tagcgagctc caagtggagg cgtggcgcaa aattacggat gctgttcatg cgcaaggagg
      901 caagattttt ttgcagttat ggcatgtggg gcgnnnnnnn nnnnnattaa gtttggatga
      961 gattaagcaa ttacgttcag agtatgtgca tgcagcaaaa caggctcagc tcgcaggctt
     1021 tgatggtgtt gaattacatg ctgctaatgg ctatctatta gatcaattct tacaagatgg
     1081 cactaatcag cgcacagacc aatacggtgg ctcgattgag aatcgctgca aattagtcct
     1141 agagatactg caagacatta ttcctatttg gggcagtggt cgggtgggaa ttcgtctatc
     1201 accctatgga agttttaatg atattgnnct ttcaatcttt cttatttgca tttgattgag
     1261 ccgcgcgcaa caacagctgg gggtagtgat caattgcttg ctgatgctcc acgaactggc
     1321 cagttattcc gtccattatt tgctggcaag gtggtattgg cgggtggctt tgatcaagcg
     1381 ggtgcagaaa aggcaattgc cgatggggag gctgatgcca tcgcatttgg aagactcttt
     1441 atctctaatc cagatttgcc aagacgtttt gagttaaatg ctgagcttaa tccttataac
     1501 cgcgcgacat tctatggcgg taatgagaaa ggctacaccg attattcgac cctttgagcg
     1561 cattcattac aatgaagaag atcatcgaaa aacatacaaa gatcaaaata gactatggcc
     1621 acctatcaaa ctgagcgcgt actcaccgtt aagcattgga acgacagcct ttttagtttc
     1681 acaacgaccc gtaatcaaag tttgcgcttt agaaatggtc atttcttgat gattggttta
     1741 gaaatcgatg gcagaccttt ggctcgtgcc tatagtgtgg ttagccccaa ttacgccgag
     1801 cacctcgagt tctttagtat caaagtaccc aatggccccc taacatcgcg tttacaacat
     1861 attcaggtag gcgactctat cttggtgagc gagaagtctg tcggtacctt agtgattgat
     1921 gacctgaacc ccggtaagca cttgtatcta tttagtactg gaacaggatt agcaccattc
     1981 ttaagcatca ttcgtgatcc agatacctat gaacgctttg agaagattgt cttaattcat
     2041 ggggtacgtg tggttaatga acttgcgtat caagagtatt taacgaaaga gttacccaat
     2101 gatgagttct tgggtgagct tgtgcgagag aagttaattt attaccccac ggtaacgcgt
     2161 gaagcttatg ctcatactgg cagactgact actgcgattg agtcaggcaa attatttgcg
     2221 gatattggtt tgcctcccct cgaccccaaa gtagatcgcg ccatgatctg tggcagcccg
     2281 gcgatgttaa aagaaaccag tcaactctta gatcagtgtg gctttaaggt ctctccctcg
     2341 tcaggcgagt tgggtgatta tgtctttgaa cgggcttttg tcgagaagta gccaaaaaag
     2401 aagccccttg cggggcttga aattcaaagg aaataaatca gtctctatta cagattaggg
     2461 ctaccgatct ggctatacag actaatcagt aattaaaaac ccttaggcta tcgttcttca
     2521 tcgcctggct gtagagcctt ttattagctt ggtcctgtga tttcaggcat taggcactag
     2581 aatttaccct agtcagcccc catagaaaaa gtatgcgaat cgaagataaa gaccctgata
     2641 gacataatgc tatcgagtac ccgatggagg tcggtgctcc taaatttgca cctgtgccca
     2701 ttaaggaaga aaaagataag gcaattaata ttgctcggca gcatgcgaat caagagtacg
     2761 accgcattat ggagcaggcc gcagtcttaa tgcgtcaggc taaggcactg caagaacgat
     2821 tggatgcaac cgagatggtg catgccgcca aatttacctt taacccagtc tacggaaaaa
     2881 aatattacct ctatcaagac caggcaatta gtggatatcg tttagtgcag aactcaccgc
     2941 gcgaatggag ctgcggtatt ccagacgggt ggtccttttg ccaagcagtg cgcaagatgg
     3001 gcgatagtac gtgggaaatc gttgaggaag atttgcaata aggatgccat atgaaaaaac
     3061 ccgatagccg ttgttgtgga tcaggtgttt gcatcatcaa tgatgctggc gaatgttggt
     3121 gtggccaaaa atgggatggt gaaaaaatgt c
//
LOCUS       DDPQ01000046           34255 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_1454,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000046 DDPQ01000000
VERSION     DDPQ01000046.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 34255)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 34255)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..34255
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_1454"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    735..780
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2900..2915
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7377..7441
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    28556..28651
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9678 a         8608 c         7511 g         8229 t
ORIGIN      
        1 aacatcgtgc gttacgtcga tgagggtatg gatccactca aggcagcgct caaaggtagt
       61 cgggaggttg gctttacgat catttcgatt tctttatctt tggtggcggt ctttattcca
      121 ctcttcttta tggagggacc gattggtctg ctatttagag aattcgccgt cgtggttact
      181 ctagctattc tagtttcagc agtggtatca ctcacattgg tgccgatgct ttgcagtcgc
      241 tttctaccaa aaccaggtca acacccaccc gagtttgcaa ttaccaagca gtttgatcgc
      301 tggtttaatt ggactcagaa aacctacgtc cactatttag acctagcctt agaaaagcgt
      361 caacgcgttc tctggatcgc gattgctact tttgtgatca cgatcgctat gtttattttc
      421 actcctaaag gcttttttcc ggaggaggat attggtcaaa ttacagtgac caccgaagcc
      481 gatcaagaca tctcgtttaa agccatgttg gacttacaag acaaagcagc agagatagta
      541 gccaacgacc ccaatgtggc gaactttgtc tcgattctgg gtggcaatct cagctctgga
      601 agtaatacag gccggttctt cattgtgctc aagccccgcg gtgatcgcga atccatgaag
      661 aaggtcctag aggggttgcg gataaagttt agagatcttc ctggcctgca agtttttatg
      721 cgtcctgtgc agaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 tacgcagcga ccccatcttc cgcgatgtca ccagtgactc ccaattaaag ggtctaaatg
      841 ttcaaattga tattaatcgt gagaaagctg ctcaatcagg cgtaaccata gcggatataa
      901 gacaggcttt atacaatgca tttgggcagc gccaggtctc gactatttac accagcgtca
      961 atacgtatta cgtgatctta gagaccgccg aggatcagcg ccagtttgaa acggatcttg
     1021 ccaaagtatt tgtgcgagga cgctccacca atcaactcat accgctatcc agtcttgctt
     1081 cattcaagcg cactattggt cccactgcag ttaaccatca gggccagatt ccagcggtga
     1141 ccctctcctt taacttagct ccagatgttc cccttggtga tgcaaccgac aagattgaga
     1201 aatacgttaa agacattcaa ttaccgccct ccatcattac aagctatggc ggtgatgcta
     1261 aggtatttaa aggtaatcag ggcggtcaat ttatcttgat cattgcctcc ctgctggtta
     1321 tttatattct gttgggcgtt ctctatgaga gctatatcca tcccattaca attttggctg
     1381 gtttaccgtc tgctgcaatt ggtgcgctac tttcactttg gatgtttgga atggagctca
     1441 cgatcatcgc cacgattggt attttgatgc tgatcgggat tgtgaaaaag aatgccattc
     1501 taatgattga ctttgccttg gatgcgcaac gcaatcaaaa catgagtcct actgaggcaa
     1561 ttcgttcggc atgtatccta cgtttcagac cgatcatgat gactaccttg acagctatcg
     1621 taggcgccct acccatcgcc cttggcttag gtgctggcgc tgagttacgg caacctctcg
     1681 gggtgagcgt cgttggtgga ttaatcctct ctcaattcgt tactttnncc cgagatgctg
     1741 aaagacacct aaagacctta caattaggca tgcgtcaaat tattctagat acagagacaa
     1801 ccggacttaa tcccgccacg ggtgatcgga ttatcgagat tggttgtatt gaacttatca
     1861 accgtcgcca aactggtaaa acacttcatc actacattaa tccagagcgt gatatcgctg
     1921 agggtgcgtt tgcggttcat ggactctcca gagagttctt atccgacaaa cccttgtttg
     1981 cacatattgt ggaagagctg acagagttcg taaaagatgc cgaggtgatt attcataatg
     2041 cgccatttga cctgtcattc ctcaatactg aatatgcctt gttaaagcga ccgatcttta
     2101 cagagcatgt tgagaaggta gtggatacct tgatcgatgc aagacgtatg ttcccaggaa
     2161 agcgtaactc tttagacgct ttgtgtgaac gctttgcgat tagtaatgaa catcgcacct
     2221 tacacggcgc cttactcgat gcccagttat tggccgaggt atatctcgcc atgacccggg
     2281 gtcaggagga tctcaccatt gatttaattg attacagtac taacggctta aatggtttag
     2341 aacgagctcc tctaccaagc aatctagttt atttcaaggc tagcgatctc gactgtgagg
     2401 cacatagcaa agcccttgag gagatcggca aggctagcaa aaagaccccg gtctggggtc
     2461 tttagagcgc ccaactactc gtgtattaaa tcgctgcagc gattgcctta cctaaatcct
     2521 cagtactcgc tttgccacca atatcagggg ttagtggtgc atgccccggt cctgccgata
     2581 agactttctc gattgcatca aataccgctt gaccagcctc tggatagccg agatggtcta
     2641 gcattaatgc agcgctccat atttgtccaa ttggatttgc aatcttcttg ccgtaaatgt
     2701 ctggggcaga gccatgcact ggctcaaaca gggatgggaa cttaccctct ggattaatac
     2761 tggccgatgg cgctactgca atcgttccgg tacatgcagg tcccaaatca gagagaatgt
     2821 cgccaaacag attggaggca accaccacat caaaacgatc tgggttcatc acaaactgtg
     2881 cggtcaagat atcaatgtgn nnnnnnnnnn nnnnngtggc tgatgtcagg tgctttttgg
     2941 gtcggctctg agctagctca taggcaaact taagaatacg atcgacaccg tgccgagaga
     3001 agacagattc ttgcaccaca atctcacgct ctgtatcagc aaacatcttt ccaccaactg
     3061 ctgagtactc tccctcagtg ttctcgcgca ctacaaagaa atcaatatcg cccggtttgc
     3121 gattagcgag tgggcatggt actcctggta gtaaacggac tggccgtaaa ttaacgtatt
     3181 ggtcaaagcc acgacggaac tggatcaagc tgccccagag tgataaatga tctggcaaga
     3241 tattgggcat accaacagcg ccaaagaaga tagcgtcata cttcatcaga gtatcaaacc
     3301 aattgtctgg catcatcttg ccgtgctttt gatagtaatc gcaacttgca aaatcaaagt
     3361 gatcaaactg aaactgaatc ccgaacttcc gtgcggctga ctctagggca cgaacgccct
     3421 ctggcatcac ctctttaccg attccatctc ccggaataac ggcgattttt ggatttacta
     3481 tttttttctg gatgttcatt gcttacggtc tcgattaatg gctaatggat ctgattttac
     3541 tagctgattg ctcgccgaat ctcacgatcg gccttacgtt ctttctctct tgcctcttca
     3601 tcggtttctg cggccggaat agaaagagcc gcctctacca ttcgtagatt atcttttggg
     3661 caaataggag ctccgtaact agaccaacgc tttaagaggg ttacctcaaa accgcactgc
     3721 gggcaggtcg ctttattacg acttagatta cttgatcttg ggggtggaaa aacaatagct
     3781 tgatggggat aaggtcctaa tttttggcta atggtcatta gacgcacttg caactcttca
     3841 gaggcatgcg ccatgcgagc tggcccctct aaaccaaccg attggcaaat ccctttaaag
     3901 tcctcaccat gaccgctgtg acagtcatcc actgcatgac ataactcgtg caccaaggta
     3961 tctaacaatg caactgggtc atctaatttc ggtgaaataa agatctcatt aacgccacca
     4021 cccgatcgct cacgaggcca acactgacct agggtagtcc gcgggctact ggaggctgga
     4081 aaaccgcaag agactcgaac tggtggaatg gcatagcctg cctttgagaa aatgggctct
     4141 aaatgacgaa caccgtcttg aagccaagct tcgcgggttg tgtgtggaac ggctaactga
     4201 ctcatgatct gattctggaa ggggtatatg aattggggta ttctaaaagg gaacaatttg
     4261 tagatctgtg agcataaacg tatttcttaa agagggatcc attccttgaa gaatctagct
     4321 aatctcagtg ccagccaagc agccagagcg cttgcccaac gagaaatcaa agccgaagat
     4381 ttactactcg attgtctaaa tcaaattggt ttacgtgaga accaggtgca tgcctgggtc
     4441 agtttgggta aggaacatgc catccgcact gcccaagaac tagaccgagg tgcggtacaa
     4501 ggaatactgc atggcttacc gattggagtt aaagacctct ttgatacgca tgatcttccc
     4561 actaactatg gctcaccgat ttatggcaat aatcatccgg tatgtgatgc ggtatctgtc
     4621 tccttgatgc gagaagcagg agcaattgtt ttaggcaaaa ccgtgactac ggagtttgct
     4681 agctttaagc ctggtcctac ctgcaatcca cgcaacttag atcacacacc agggggctct
     4741 tccagcggat cggccgccgc tgtagccgac tgcatggtac cgctcgccac gggtagtcaa
     4801 actgcagcat ccatcattcg acctgctagt tattgtggag ttgtaggata caaacccagc
     4861 tatggaaaaa tcagtatcgg gggtgtaaaa agtttatctg tatcactgga cacgcttggt
     4921 gtttttggaa gaactgtatc cgatgtggct cttggcgttg ctgccatgtc agccgatcat
     4981 gatcttgcta agatcgaccc gctcgagcat aaggttcgca ttggtatttg tagaacatct
     5041 gatttcgcaa tggctcaagc agaaactgca gccgctctct ccttagcagg ctttgcagcg
     5101 aagtcctttg caaaagatgg cgtttctgag attacccttc cgccctcttg cgcaacgcta
     5161 actgagatcc agacaacgat tatgttgttt gaaatggcta aaagctttac cttcgagaaa
     5221 cttaatcatc tggatcaaat tagtacaacc cttcaaacca ttattcaacg cggtgatgcg
     5281 attaaatact ctcaatacag tgaggccttg acccaggcgt ggcgagctgg cgcagagctc
     5341 tgccaagcat ttgaagatca attcgatatt ctgattgcac caagcgctac aggcgaagct
     5401 ccgcacacca tagaacaaac tggcgatcct gtatttagtc gtggctggac cttaatgggt
     5461 ttaccctgca tcaacttgac agtcaccagc ggtcctaagg gtttgccggt gggcgtaacc
     5521 ttagttgcag gtccaagacg ggatcgccta ctactcagtg tggcacacgc cttagcgcaa
     5581 aacctatcag acccgctagc actcaatcaa gcttgatgtt ggctgattta atcgcctttt
     5641 gcatcaatgc aatttctttc ttgagtaagg catcggttcc ctgcggatta agatagcgta
     5701 cctcaatccc tgctgcatcc cctcgttgct tggcttcagg aagctctaaa gcctttttaa
     5761 catcctgcgt taatttattc acgatcgctg caggtgttcc agcgggagca tacaaagcaa
     5821 cccaagactc gagttcaaaa ccaggtannn nacagctaaa gcctttaatt tgccagtttg
     5881 gacatgcccc atgacagaag gtggcgttgt aataaaaacc tgaacctgcc ctgccagtac
     5941 gtcctggatg gcttggcctg atcccttgta tggaacatgg gtcaacttaa tgccagtggc
     6001 ttgctcaaag atctctgtac caatatgcga taccgaaccg ttaccttgtg aggcatagtt
     6061 cagcttacct ggattagctt ttgcataagc aataaactct ttaagattat tggctggaac
     6121 tgatggatga accgcaatga cattcatcgc aactgtcagc aatgcaatgg gagtgaagtc
     6181 tttaaacgga tcccaactta atttatccat aagcaatgga ttggcggcat ggtaacccga
     6241 gtaagaagcc aataaggtat agccatcggg cttactctta gccacatatt ggtaggcgat
     6301 attaccgctt gcaccttggc ggttatcaat gatgatcgat tgcccagtca ctcggttaag
     6361 aggctccgtt agtaaacgag ccgatgcgtc gaccaatcct ccaggaggta cgggtacgac
     6421 gatcgtgatg gggcgatctg gaaatgtttg tgcatgggtt atgccagcca gcatgaatac
     6481 agtagctaaa aatcccatga tttgtttaag ttgcaagagt cttactgtgc gcatgtgtcg
     6541 tctccaaaaa ttatgttcat cattatcgta atatcttaac gctgtcccat tttgacatcg
     6601 gcaaatactg cctgagcctc acgcggtagg caacgccagt attgtgggct agctgataca
     6661 tgcccctgta atgcagcggc tgcctcccat ccccaccggg gtttatacaa gaaggctcgg
     6721 gctaaggcaa ttaaatccgc ttcgtgattt tgcaagatct cttctgcttg aattggctca
     6781 gtgattaagc caaccgccat ggttgccata ccggtttttt gcttaatctc ttttgcaaag
     6841 ggcacttgat aattaggccc caactgaatt ttctgtttag gggagatacc gccagaagaa
     6901 acatggatgt aatcacaccc caaagacttt agccgttgtg tgagctcaac cgactcttca
     6961 agactccaac cattctcaat ccaatcagat gccgagagcc taactcctaa gaccccgccc
     7021 cactctttac gtaccgcaga aaatagctcc agcacaaagc gcatccgatt ctcaagggag
     7081 ccgccgtatt gatcctgacg ttgattggca atcggcgata agaactgatg caataaatag
     7141 ccgtgagcag catgcaattc aattccatcg atcccaattg ttttcgctcg tctggctgac
     7201 tcgacaaagt tcttaataat gacttgcatc tctgccgtgc ttaactcatg gggattacgc
     7261 tcaccatcta gctgaggaat tgcggagggc gcctgggtct cccagccgcc attctctgat
     7321 ggaaggatct gaccgccttg ccatggtgct gcacttgagg ctttgcgacc agcgtgnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nagccctaaa caatagggag tgatgcggcc ttcgggactt actgctgtgg cttcaataat
     7501 aaagagcccc gccccgctgt tcaacaaatt accccagtgc attaaatgcc aatcacttgc
     7561 ctcaccctct actgccgagt attgacacat aggcgccacc acgactcgat tcggtaattg
     7621 catagagccc tctggggcag gtagtgaata gtctgaaaat aagaggctca tgggataaat
     7681 gaccaaaagt gattgaaagg attaaaatga taccaagtga ttcttaagac cctttctggt
     7741 tgccaagaca atctaggccc aatcatcaat acgagacata attaccatga acgctccaca
     7801 agcctttgaa attaaagaag atattgctca ctatatcggt ggtcaagttg ttttgccaca
     7861 aggcacacgt tttgccgatg tgcacaaccc tgccaagggc tcggtagccc gtcgcgttgc
     7921 tctcgctagc aaagccgatg tgggcaaggc tgttgctgca gcacaagcag cctttcctgc
     7981 ttggggcaat acctcgccgc ttcgtcgcgc ccgtattctc tttaaatacc ttgaccttct
     8041 gaaccgtcat cgcgatgaac tcgccgccat catcaccgct gagcatggca aagtctttac
     8101 tgatgctcaa ggtgaagtca cccgcggtat tgagatctta gaatttgcga ccgctatccc
     8161 agaactctta aagggagatt acaccgagca ggtctcaacc gatatcgata actgggttat
     8221 gcgtcaacct ttgggtgttg ttgcaggcat tacccccttc aattttccag tcatggtgcc
     8281 gatgtggatg ttccccgttg cgattgcatg cggcaatacc ttcatcctca aaccgagccc
     8341 gaccgatccc tctgcctcct tattgcttgc gcgccttttg aaagaggctg gattgccaga
     8401 tggcgtcttt aatgttgtgc agggtgataa agaggctgta gatgcattga ttgagcatcc
     8461 agatgtcaga gcaattagct ttgtgggttc gaccccaatc gctaattaca tttatgagcg
     8521 cggtgctcat tttggtaagc gcgttcaggc tttaggtggc gccaagaatc atatggtggt
     8581 catgcccgat gctgatattg ataaagcggt ggatgcctta gttggcgcag cctacggatc
     8641 tgctggtgag cgctgcatgg caatctcagt tgccgtatta gtgggcgaag cagctgacaa
     8701 agtaatgcct aaattaattg agcgtaccaa aaccttgaaa gtaaagaatg gtcttgagct
     8761 tgatgctgag atgggtccca tcgtaacgcg tgcagctctt gatcgcatta caggctatat
     8821 cgaaaaaggt gttgcgggtg gggctaagct tttagtagat ggtcgtggac ttaaagtgcc
     8881 tggctttgat caggggttct ggttaggcgg taccctcttt gatgcagtta cacctgatat
     8941 gaccatttat aaagaagaga ttttcggtcc tgtgctctcg tgtgtacggg ttgctaattt
     9001 tagtgaagca ctcgatctag tgaatgctca tgagtttggc aatggcgtag cttgcttcac
     9061 cagcgatggc aatattgctc gtgagtttgc ccgtcgcgtt caagttggta tggttggtat
     9121 taatgtacca attccggtac cgatggcttg gcacggattt ggtggttgga agcgctcctt
     9181 atttggtgat acccatgcct atggcaaaga aggggtgcgt ttttatacca agcaaaagag
     9241 tgtcatgcag cgttggcctg agagcatctc aaaaggtgct gagtttgtca tgccaacctc
     9301 gaagtaacaa ctactttttt cttgagatga acatcgctgg aaccgtcagg ttccagtaga
     9361 tggctgcgga tcgaaagcaa aagattagta agatacaagc aatcgatcct ggcattgcat
     9421 actctgggaa ataatttagg agtaagacat aaagcatgca accgagtgat acgggaattg
     9481 catatagctc ataggacatt aataaggttt tgcgacctgc caatacatcc cgaatcaatc
     9541 ccccaccaat tgctgttaca acccccaaga taatggctgc ttcggaacca ccgaaatctt
     9601 catgccaagc cttatccgcc ccctgtatcc caaacaaagc ggcccccaga ccatcgatat
     9661 aaagaatggc tttatacaac tgaggctgag aaagtattga ctcggaataa aaggtaatga
     9721 tgctggcaat tgcagcaacc aagatataaa taggtgcatc ggcccaaaag actggcaccc
     9781 ccataatcac atcccgaatg gttccgccac caatggctgt gatgattcct aagaccagaa
     9841 ccccaaagag atcaacgccc cgctccgaaa tagcaagaac acctgtaatt gcaaaggctg
     9901 ccgaggcaac gataccaacc caaaaactaa gctcttccat cgagccttcc tagtcaataa
     9961 atagagagga agtaattatt gcaaagtatt tttgaggagc ggcatttctg ggagggctag
    10021 tacatcaatc ccctcctcac gtaaggcact tacctcatcc gcactggtct gcccccgaat
    10081 actccgctca ggagtttcct tgtaatgtat ctttctggct tcttctgcga atgatgagcc
    10141 cacatcctct gatttattga ctagatcacg catggccttc ataaaggttg cttggatctg
    10201 cttctccaat tggccatgat cagcattgga taaagcaatc gtttgcgcag gctctgttgt
    10261 caccgaggcg ggctgcaatt gctccggact agctctgcca ccaatatgcg gtgccgatgg
    10321 taagcgtcgc acctcagcgt tatcgcaaat ggggcagcct aagcttccac ccgactgctg
    10381 gctcaggaaa tcgtcttcag acacaaacca gccctcaaag tgatgctcat gagcgcaagc
    10441 taagttataa actttcatgg gtaatagatg ggggcctctg gcctgaattc aagagagaaa
    10501 gagtattttt gtagataatg ccagtttaat gctttatggc ttaggagttt gatgaggcgc
    10561 gtcggcgacg ctctaaccag taagtcgaga tgatagtttt tacatccgta atcttgccag
    10621 attctaccca ctccaagagc tcttctaagg gcgctgcaaa gacgtccaaa aactcttcct
    10681 catcaagtcg tgccggacct gggattaagc tctcagccaa atagatatca atgaactcgg
    10741 ttgaatacga aatcactgga tgaatacgcc taatcttgct ccagcgcttg gcggtatagc
    10801 cggtctcctc ttgaagctcc cgctgagcac acaagagcgg atcttcgccg acatttaact
    10861 taccggcagg gatctcgatc acagcctgag agattggata gcggtattgg cgctccaata
    10921 aaatacggcc atcatctaat acagccagaa tagcaaccgc tccagggtgc tctaggtact
    10981 cgcgtagggc atgctgccca tctggtaaag agacggtatc gcgcttcatt ttgaggaaga
    11041 tgccatcgta gaccccttcc ccggatatcc ttttttcgac gagatgatcg tcgtcgtggg
    11101 cgaggtcttg gaaggatttc tcgctcacgc tctactctct atttaagaac caatgagagt
    11161 gctgcgcatc gcattcaagc acaagctcat gagtccagca gggaatattc cgagaataag
    11221 gatcattaaa ccgttaatgc caagaatacc cttcgcaaaa ctggtgccat tcacagactg
    11281 ctcatgcacc ggctcatcaa aatacatgac cttaacaacg cgcaaatagt agaaggcacc
    11341 gattaaggaa gcaatcactg cgatcacagc caacacgatg aaatctgcat ccaccaaagc
    11401 ttccaataca gagagtttgg ctgcaaatcc aactgtcggc ggaatcccgg ctaatgagaa
    11461 catcatgacg agcccaataa atgcatacca aggatgctta cggttgagac ccttcaaccc
    11521 ctccaaggtc tcacagtcat agcccttgcg cgagagaacc atcaggaagc cgaaggtacc
    11581 caaggtagta atcacatagg taatggcata gaacatcgag gcactgtatg catgatcatc
    11641 aaagactgac aacataccca gtaatacaaa acccatttgg gcaattgctg agtatgccaa
    11701 catccgttta aggttggttt gcgcaatggc tgtgacattg ccgagtacta gagatagtac
    11761 tgccaagatc attagcatcg gctgccaatc tgaaatcaag ggcaataagg tattaatgag
    11821 caagcggaat agcaaggcaa acgcggccag ctttggggct ccggcaatca gcaaggtcac
    11881 tgctgtcggc gctccctggt aaacatcagg aacccacata tggaatggca ctaccccaaa
    11941 cttaaaggct aagccagcca caatgaagac caggccaaat gccatcacta agtgattaat
    12001 tctggggtcg agcaggagtc gtaggatctc tagtaaatcc agtgacccgg ttacaccata
    12061 gatcattgac atgccgtaga gcaagaatcc agatgctaat gctcccaaga caaaatactt
    12121 aatagcagct tcagaagaga ggccgctgga gtggcgcatc gccactaatc cataggtgga
    12181 cagcgccatt aactctaagc caaggtataa ggtaagaaga tttgctgcag agattaagac
    12241 cagctgtcct aacaaagcaa atagggttag cacaatgaag tccggtctaa acaggccccg
    12301 atcaatcaaa tattgcttgg agtaaataag gctgaggagt aaggcaaagc aagatccagc
    12361 ctttaacaaa ttagcaatcg catcggactg gaacaaccca gccattgcaa ggtaaggaag
    12421 atcagcgatc cgtgtaatga atgcggcacc caaaccaatt aagagaaggc aagccaccga
    12481 gtacacatag gatgcggctc taggagcatg aaatacatcc tgctcaggac cgcccttcgc
    12541 ttctttaaca aacgcactac tgaccaacaa taagcagctg atcgtaatta agacgagctc
    12601 tggagcaatc gcgataaggt caaaggagtc cataaatgta ctttactcag agtttgctaa
    12661 tagcgacatg ctcaagcagt tgcatcactg ctggatgaat aatgtcggtg aatggttttg
    12721 gatagacgcc cataccgatc acgcagatcg acaaaacaat gaacatcata aactcacgat
    12781 tatttaggtc caccaagcca tcaacctgag gattggtaat ggcgccaaag ataacgcgct
    12841 taaccatcca tagggaatat gccgcaccca ggatcaaggc agtggccgat aagagtccaa
    12901 tcatgaagtc ataatcgaca gcggccagga tcaccataaa ctcccccaca aatccagagg
    12961 ttgctggtaa gccacagttg gccatcgcca tcaataccat gaaagcggag aacttaggca
    13021 tccggtgaac cacgccacca taatccgcaa tctggcgtgt atgcatacga tcgtagagaa
    13081 cgccaatagc caagaacatc gcacccgaaa caaaaccatg agagatcatc tgaacaatac
    13141 cgccctcgat acccaaagga ctaaagataa aaaagcccaa ggtcacaaaa cccatatgtg
    13201 cgatcgatga ataggccacg agttttttca tgtcctgctg gactaaggca actaagccaa
    13261 cataaatgac cgccaccaag gataagaaaa ttatgaaagg ggcaagatac tgacttgcat
    13321 ctggcgctat cggcaaggag aaacgcaaaa atccatacgc acctagcttc aacataatag
    13381 ctgccaagac cacggagcca ccagtaggcg cctccacgtg cacatcaggc aaccaagtat
    13441 gtagcggcca catgggaacc ttaaccgcga aggccatgaa gaaggcaatg aagagcaaga
    13501 cctgctcaac aatatcgagt ttcgtgtgat gccaagctaa tatgtcaaag gtattgctaa
    13561 ggttgtacaa ataaagcaat gctattaagg cgagtaatga tcccagcaag gtatacaaga
    13621 agaacttgaa tgccgcatag atacgatttt taccacccca cacaccgatg atgatgtaca
    13681 tcgggattag agtagcctcg aagaatatgt agaagagtaa accatcaagc gcagagaaca
    13741 caccgatcat caaacccgaa aggatcatga aggcggcgta atactgcgat accttctcag
    13801 taatcacctc ccaagccgca atcaccacaa aaatattaat gaaggcggtc agaactacaa
    13861 accaaaccga gataccgtca actcccaaag agtaattaat gtcgtagcgg ggaatccaac
    13921 tgaacttctc cacaaattgc atgcctggat ttgctagatc aaaattagta atcagtggca
    13981 atgtagcgat gaatccaaga atagcgccaa ataaagcaag ataacggacg ccagccgatg
    14041 gacgctctga gccatagatc aagatgatca gaccaaaaac aatcgggatc caaatggcgt
    14101 aagaaagaat catggtgaag gctttactta ataagcgata taaatgtggg tatacaaaat
    14161 ccaggctaac aagctaacta agcccaatat catggcaaag gcgtagtgat ataaataacc
    14221 cgattgcaat tgccgaatca cgcccgagaa cttaccaact aaatgtgcac taccgttaac
    14281 cacccaacca tcaatgaccc gttgatcacc ctgcttccat aaaccaccac cgatcaagag
    14341 tgcgcccttg gcaaatatcc attgattaag atcatctaag tagtacttgt tatccaaaat
    14401 tttcttaaga ggagcaaaga ctttggcaat cttctcgggg agtgaaggaa cccataaata
    14461 acagatagca gccacaagca ctccgagcgc caagagtaaa aatacggggg tcgtaaatcc
    14521 atgcaaggcc atcgccactg gtccatgaaa atgggatgcc agggtcgcca tcgccggatg
    14581 ctttaccgca tccacaaaaa ttgcatcccc aaagaactta ccaaagagca taggatcaat
    14641 ggtgtagtaa ccaataatga ccgatgggat cgctaatgcg atgagaggta gagtaactac
    14701 ccaaggagac tcatgaggtt tctgtcccgg tgcaagccca tggtgttcat catggccatg
    14761 gttatggtca tcgtgaccaa atcgctcttt gccatgaaac acatagaagt acaagcggaa
    14821 ggagtacaaa gcggtcacaa agacactcag catgaccgca aagtaagcaa agcctgagcc
    14881 aggaatagta ctagcggcga ccgcttcaat aatcgagtct ttagagtaaa aaccggagaa
    14941 gaatggtgta ccaatcagtg ctaacgatcc taatagcatc atcaagcagc taatcggcat
    15001 atgcttccat aaaccgccca tcttacgcat atcttgctca tgatgcatgc caataatgac
    15061 gctaccagca cccaagaaca ataaggcttt aaagaatgca tgggtcatta aatggaatat
    15121 ggcgattgga taagcagata ctccgagagc aacggtcata taacctaatt gtgagagtgt
    15181 ggaatatgcc accaccctct taatatcggt ctgcacgatt cctaaaaagc ccataaagag
    15241 tgcagtaatc gagccgatga ccaaaatgaa actcaaggca acatccgata gctcaaagag
    15301 aggtgacatt ctgctaacca taaagatgcc agcggtcacc atggtcgcag catgaatcaa
    15361 tgcagagatc ggggttgggc cttccatgga atctggtaac cacacatgga gcgggaactg
    15421 ggctgacttg cccattgcac caataaataa acagatacag gcaactgtta tcaagttcca
    15481 gctcgtacct ggcactgttt gagcagccat cacagtattt tgggcaaaga tatcggcata
    15541 gttcatagag ccagtagcgg ccaataacaa accaatccct aatatgaagc caaagtcacc
    15601 aacccggttc accaagaaag ctttcatatt ggcaaagatc gcagtgggtc ggtcgtaata
    15661 aaaaccaatc aagaggtaag agactacgcc taccgcttcc cagccgaaga acagctgcaa
    15721 catattgtta ctcatcacca gcatcagcat ggcaaaggtg aacagggaaa tataagcaaa
    15781 gaagcggtta tagccctcct cgcctttcat atagccgatg gtgtagatat gcaccataag
    15841 agatacaaag gtcacgacaa ccatcatcac agccgttaga ggatcgatta agaacccaat
    15901 atcaaaactg agctcgccta atttcatcca ggtataaacg ctaccatcaa aatagtagcc
    15961 attatttact tcaaccaaga cccagcacga aagtattaag gaaattgcaa cgcctaatat
    16021 ggttaaggac tgggttgcaa tctgtccaat tcgattacct aagaatcgag ttcctaataa
    16081 accagccatg gcagaaccga ccagtggggc tagcggtatt gcgcagagat gggctgtagt
    16141 taaggaggat gccatacgga ttagcctttg agatgatcca agtcttcggc attaatggtg
    16201 tccactttac gaaacagaac caccaggatt gctaaaccaa tagcggcttc tgcagcagcg
    16261 acggtcaaaa tgaagaatac aaacacttgt cctgccatat cgcccaaata atgggagaaa
    16321 gctacaaagt tcatattgac ggccaatagc attagctcaa tagccatcaa caaaataatc
    16381 acattacgac gatttaaaaa gataccaact acgctaattg caaagaggat ggcaccaagg
    16441 acaagatagt gggcaagcgt gatggtcatg atttactctc ggtctcagac gatttagact
    16501 ttcgatcctg cttagcctca ttactcgaat ccatggatac aatccgcatc cgatcttttg
    16561 ccttaacggc tacctgctcc gcaatgtttt gtcccttaac gtccttgcgc ttacgcaagg
    16621 tcaatgccac cgcagcaata atcgccacca agagaatgat tccggcgatt tcaaaggcgt
    16681 acagatagtc tgtaaacatt agatatccca aggcctgggt attgttagca gagaccactt
    16741 ctggcaggat attgactggc gaactggtac caataaatcc gcggattaga acaatcgata
    16801 actccaagat gatgaccacc ccaatgagca ttgctaaggg gaggtatttc ttgaaatcac
    16861 ggcgtagatg ggctaaatca agatcgagca tcatcaccac aaacaaaaat agcaccatca
    16921 cggcacccac gtaaaccaag atcagaatta aactaagaaa ctcagcctta agaagcatcc
    16981 aaatacctga tgcgctaaag aaggccaaga ccaggaataa ggcagcatgc actgggtttg
    17041 ggacggtcac tacgcgcaat gcagaaataa ctaacaggcc agcaaaggcg tagaagaaca
    17101 ctgcgaagag agtggatgga tcaaagttca taaatcaatt atcgatatgg tgcgtcggcc
    17161 tcacggtttg ctgcaatttg cttttcatac tcgtcgccaa ccgccagcaa catctctttc
    17221 gtgaaataca aatcgccccg cttatcacca aagtattcaa agatattcgt ttcgacaatc
    17281 gcatcgactg ggcaagcctc ttcgcaaaat ccacagaaga tgcacttggt taaatcgatg
    17341 tcatagcgcg tggttctacg agtaccatcc tcgcgttgat ccgactcgat tgaaatagct
    17401 aaagcaggac acaccgcttc gcatagtttg caagcaatgc aacgctcttc cccattcgga
    17461 tagcgacgta aggcatgtaa acccctaaaa cgtggcgaca agggtgtctt ctcttcaggg
    17521 tactgaacag taatctttgg cttaaacaaa taacggcctg tgatagacat accagtgagc
    17581 acgtctttta gcatcaagct atctaagaac tgggagattc gtttaaacat aatcgcttag
    17641 ctccagatat tccatgggga caaaatccaa gctccaacca caaggaccca aaataccgtt
    17701 aaggggataa agaccttcca acccagacgc ataatttggt catagcggta acgtggcaaa
    17761 gtcgcccgta accaaataaa gcaagaaagc aagaagaagg tcttggcaaa taaccagaag
    17821 aagcctggga tatcgcgtaa gaccggtaca tcaagaatcg gtaaccaacc gcctaagaac
    17881 atggttgatg ctaaagccgc aatcaagatc atattggcgt attcagcgag gaagaacaat
    17941 gcaaatgcca tacccgagta ctcgaccata tggcccgcaa caatctccga ctccccttcc
    18001 acaacatcaa atggatgtcg attggtttca gcaacacctg atatgaagta aatcacgaac
    18061 attggcaaaa gcggtaacca gttccaagaa aggaaattga gtcctatgct ggcgaagtaa
    18121 ccgacctctt gcgaatgaac gatattgctc aaattaagag agcctgcaac cattaataca
    18181 gataccagtg caaagcccat agcaatctca tacgagatca tttgtgccga tgcgcgcatt
    18241 gcacccaaga aaggatactt cgagtttgat gcccagcccg ctaagatcac gccataaaca
    18301 cccaccgagg taattgccat gatgtagagc aaaccagcat tcacatctgc tagaacaagt
    18361 tccgcctgaa atggcattac tgcccaagca gcaaacgcag gggtgagcac catcaagggt
    18421 gcaatgatgt ataaagcatg acttgcacgg gccggagaaa taatctcctt catcaatagc
    18481 tttagggcat ccgcaattgg ttgtaataaa cccaaggggc caacgcgatt agggcctaaa
    18541 cgaatatgca tccagccaat tagcttgcgc tcccacaagg tgaggtatgc aaccgcagca
    18601 aacatcggca gaacaatcac cacgatctta accaaggtcc acaccaatgg ccatgtggga
    18661 ccaaagagct ctgcgccttg ggtcgtgatg gtatttaaaa gatctgtcat gattatcttt
    18721 aagcagtaac cgccactttg gtaacggata cttccccaaa catgggacct aattgacagc
    18781 tgagctccgt tgcaacggag atacgcacca cgccatttgc caaaccaggc attaaggcca
    18841 cagaagcctc gatcgtttga cctgcttggc ttacagtaac caaatctccc tcatttaagc
    18901 ccaattgact caacaaagcc tcacccaggc caacttgtct ggctgacttt gcatcacgtg
    18961 tgagctgtaa agatggtgca cgacgcacga ttgcatcaga ggcataaatt gcttgatccg
    19021 caatcctctg gatcgatcct gatagagggg tcttgttaat aacctgcgcg tttgctgtaa
    19081 agccgttatc aagggcctct gcaagcggtt ttgtaagagc atccgcataa acctcttcgg
    19141 gtaggttata caagaagcct tctaatttaa gaagactacc aattgctcgc aatactttcc
    19201 aagcaggtct agcatcggct agagggcgaa ctgcaggctg aatggtttgc gcctgccctg
    19261 cgagatttac aaaactgccc acggtctcgg tatagggcgt aatcggcaaa atgacatcag
    19321 ctaactcaag cagatcaggg cttgtatacg cactcatcgc aatcactgta tcggccttag
    19381 agagtgccgc acgggtttgc tgaggattag gtaaatccat caagggttca atattcatca
    19441 aaacgtaggc atgcgcattg ctgtgcaaga tactttctac gctaccgtga ctgccttgag
    19501 tggcgccgac tacctgagca ccaacagcat taccaccctc gcttaagaat cctaatcggg
    19561 cacctgtatg cgtggcaata aactctgcca agacatgtag atcagaggca ttaggatgag
    19621 cgattgccat agaccccaag aaaatggctt gtgcttcagg gctctcccct ttagaaccag
    19681 caagcagctg atcggcaatc acttgagcct cggctgatat tgcgctagtt ggtgctccag
    19741 ttggcaatgc taatgattta gctttagcaa ccgcactcgc aatttcggtc aactgattga
    19801 tccactgact gggagtgcta tgaagatgag tggtaatcgg tattaaccaa tcattacccc
    19861 ctgcgtctaa tcgatacacc tgtaaaccgc gcttggttgc ggttcgtatg cgtgcagcca
    19921 gcaatggctg atctttacgc aaatgactac caatcaccaa aacacgctta agctgattaa
    19981 tctcggcaat tggcaacccc agccaagctc cgtgagcggc gccgcgcgta tcaatctgac
    20041 gcaagcgggt ctccacttga tcagccccaa ggccgcgcat caccttttgc aagagatata
    20101 gttcttctac gcttgagatt ggatgggcca atgctgccaa ggccttagcg ccatgctgct
    20161 cctgaatatc tcccaaggaa tgcactacat aatccagtgc agattgccaa tcggtttcta
    20221 gccattgacc attctgcttg accatcggcg tggttaggcg atcattacta tttagacctt
    20281 cgtaggcaaa ccgatcacga tcgctaatcc agcattcatt aatatcttcg ttctcgagag
    20341 cgactacgcg cataactcga ttagccttgg tttgcacggt agtgttagcg cccaatgaat
    20401 cgtgtggact cagcgagcgt ttacgcacca actcccaggt acgagcggca tagcggaatg
    20461 gtttgcttgt taatgcgcct actgggcaaa tgtcaatcat gttgccggat agctcagagt
    20521 caacggtctg accaacaaag gtggtgatct cagaatgctc accacgatta atcatgccta
    20581 gttccatcac tccagccact tcctgaccaa agcgaacgca ccgcgtgcaa tgaatacagc
    20641 gcgacatctc ttccatcgaa atcaatggac ccacgttctt gtggaaaacg acccgcttct
    20701 cttctttata gcgagagctt gacttgccat aacccactgc aagatcttgt aactgacact
    20761 caccaccctg atcacaaatc gggcaatcca aaggatggtt aatgagcaag aactccataa
    20821 ctgatttttg tgcttcgact gcttttgcgg aatgtgtgta gaccttcata ccagccatca
    20881 caggagtggc acaggctggg agcgctttgg gtgctttttc tacttcgacc aagcacatcc
    20941 tgcaattggc agcgatcgat aatttcttgt gatagcaaaa atgagggaca taggtaccta
    21001 atttattggt ggcatgcatc accatcgaac cctgaggaac ctcaacgggc tttccatcaa
    21061 cgtggatctc aatcatattg atcgcactca ttacttaggc cgcctttgca taagaagaga
    21121 ccaagcaacg tttatgctcg acgtgatacg caaactcatc catgtaatgc ttgagcattc
    21181 cttgcactgg catcgcagcc gcatcaccca aggcacaaat ggtgcgaccc tgaatatttc
    21241 cagcaacgtc cctaagaaga tctaaatctt ccgggcgtcc ttggccatgc tcaatacggt
    21301 tgacgatacg ccagagccag ccagtaccct cacgacaagg tgtgcactga ccacacgact
    21361 cttcgtgata gaagtaagaa aggcgcagga gtgagcgcac catacagcga gtctcatcca
    21421 tcacaatgac tgcgcccgaa cccaacatcg atccagcctt ggagattgca tcgtagtcca
    21481 tggtgatatc catcatgatg ttgctaggca ggaccggtgc ggatgaaccg cctggaataa
    21541 ccgccttcaa ctgcttacca ttgcgaacgc cgcctgcgag ctttagtaac tctgcgaatg
    21601 gggtgcccaa tggaatctca taattaccag ggtaggtaac atcccctgaa accgagaata
    21661 tcttggtgcc gccattattg ggtttaccta atttgagata agcctcagaa cctattgcta
    21721 acagaaaagg tactgctgca aaggtttcgg tgttattaat agtggtcggt ttgccataca
    21781 aaccaaagtt ggcagggaat ggtggcttaa agcgtggctg acccttttta ccttctagag
    21841 actctagaag agctgtttcc tcaccgcaga tataggcacc aaatccgggt acggcatgca
    21901 attgaaagtg aagttcgctc cccataattg actggccaag atagccagca gcgcgtgctt
    21961 cctctagagc ttcttcaaac cgctcataca cctcccagat ctcaccatga atatagttat
    22021 atcctgtaga gatacccatc gcataggccc caatgatcat gccttcgatc agggcatgcg
    22081 ggttataacg catgatgtct cggtctttaa atgtgccggg ctcaccttca tcgctattgc
    22141 aaactaagta tttcacgcct tggtagttct taggcatgaa gctccacttt aagccggttg
    22201 ggaatccagc gccgccacgg ccacgcaatg atgaggcttt tacttcagca atgactgctt
    22261 cgggggtaat cttctcgctc aagatgcgct ttaattgctg ataaccacca cgcgcctcat
    22321 aatctttgag acgccagttt tgaccatcca aaccagccag aattaagggc ttgatatgcc
    22381 gactgtgcaa actactcatg ccgagactcc ctttttagct tgagacttca gctcgtctag
    22441 gagagcatcg atcttgtcgt gactcataaa actgcacatc cgatgattat taacgagtaa
    22501 cacgggggag tccccgcatg cgcccatgca ctccccctct ttgagggtaa aagtaccgca
    22561 ggaagtggtc tcattaaagt taatgcctaa ttttttcttg aggtaatcgg ctgcttcttc
    22621 gccatgcgaa agttggcatg gcaaattagt acaaattact aatttgaact gaccgatggg
    22681 cttggtctca tacatattat aaaaagtagc gacctcttct accgcaatgg tcggcatatc
    22741 cagaatgccg gcaacttctg cgatgatggc cggggatacc caaccatgct cttcttgagc
    22801 agcaatcaga catgccataa cggctgattg tttttgctca ggcggatact tagcaatatt
    22861 gcgatccatc tctgcgcgag ttttggagga gaagatagag ttcatggcat tcatttatcg
    22921 atcaatctca ccaaacacaa tgtcttgagt accaataatg gtgaccgcat cagcaatcat
    22981 gtgaccgcgc gacatctcat ccatggcagc taaatgcgca aagccgggag ccctaatctt
    23041 taagcgatat ggcttattgg caccatccga catgagataa ataccgaact caccctttgg
    23101 atgctcaaca gcagcatacg cttcacctgc tggcacatga ataccttcgg tgaatagctt
    23161 gaagtgatga atcagttctt ccatattagt cttcatgtcc actcgattag gaggggctac
    23221 tttatggtta tcgctcatga ctggcccagg attagcgcgt aaccatttca cacactgggc
    23281 aataatgtga ttggattgac gcatctcctc catgcgaact aaatagcggt cataactatc
    23341 gccattcaca cccacgggga tatcaaagtt cagcttgtca tagacctcat agggttgctt
    23401 cttgcgcaag tcccattcga tgccagaacc gcgcagcatt gggccagtaa agcccatttg
    23461 caatgcacgc tctggtgtca caacaccaat acctactaag cgctgcttcc aaattcggtt
    23521 atcagttaag agattgttgt actcatcgac attggcagga aaacgattac taaaatcttc
    23581 aatgaaatct aacaaagatc cactacggtt ctcatttaaa cgcttaacag ccccctcact
    23641 tcggatcgct gacttcgtat actgaggcat ctgctcagga agatcgcgat atacaccgcc
    23701 ggggcgatag taagcagcgt gcatccgtgc accagatact gcttcataca tatcaaaaat
    23761 atcttcacgg tctcggaagg catataagaa tattgccatc gcaccaacat caagaccgtg
    23821 acatccaacc cagagtaaat ggttaagtag tcgggtaagc tcatcaaaca taacccgaat
    23881 gtattgcgcc cgaatcggca cttctacatc caataacttc tcgatagcca ttacataggc
    23941 atgctcattc accatcattg agacgtaatc caagcgatcc atatagggaa cgttttggat
    24001 ccaggttcta gtctcggcta atttctcggt agcgcgatgc aataagccaa tatgcggatc
    24061 tgctctctga atcacttcgc catcaagctc tagcaccaag cgcaatacac catgagcagc
    24121 aggatgctgt ggtccaaaat taagggtgta gttcttaatc tcagccatta gttcaatcca
    24181 ccatattgat cttcacgaat cactcggggt gttacctcgc gaggctcgat cgtaactggc
    24241 tgatagacta cgcgctttaa ctcagggtca tagcgcatct ctacagtgcc cgatactggg
    24301 aagtcttttc tgaaagggtg accaataaag ccataatcag taaggatgcg acgcaaatca
    24361 tcgtgtccat caaacaagat gccaaacaaa tcaaatgcct cgcgctcgaa ccaattagca
    24421 gagttccaga tcggcaccat tgatgcaagc aatggatagt catcgtcggg cgcaaatact
    24481 ctaaccctta agcgttgatt gtgttttaca gacagtaagt gactcacgac cgcaaatcgt
    24541 cgaccactcc acgctccatc tgcataatct ttgtagtcaa caccacatag atctattaac
    24601 tgctcaaacg agaggctggg gtcatcacgt aacaacttgg ctgcttcggc atagtgtgcc
    24661 gcatcaagca caatcgttag ctcattgtgc gcgatcacag aactcttcat acgatcacca
    24721 agcgcacggt ctaactgttg acgaagttgc tctaagcgct ctgacatctc aacccttacg
    24781 cgcaatcgtg ctggtgcgac caatcttggc ttgtaactga ataattccgt agatcagtgc
    24841 ctctgctgtg ggcgggcaac cagggacata aacatcgaca ggcacgatac gatcgcaacc
    24901 gcgcaccaca gaatacgaat agtgatagta accaccgcca ttggcacaag aacccatgga
    24961 tagaacccaa cgaggctcag gcatttgatc ataaacctta cgcaaagcgg gtgccatctt
    25021 attgcagaga gtgcccgcca caatcattaa atccgattgg cgtggagaag gacgaaacac
    25081 aacaccaaaa cgatctaggt catagcgtga tgcaccagcg tgcatcatct caacagcaca
    25141 gcatgccaaa ccaaaggtca taggccataa agagccagtc ctggtccagt tgattaactg
    25201 atctgccgag gtagtaacga aaccttgttt tagaacacct tcaagtgcca tctacgctta
    25261 ctcccaatca agggcgccct ttttccagat atagacaaaa ccaataatga attcaagcaa
    25321 gaaaacaacc atcgcaaagt agccttccca gccaatatcc ctcaaggcaa caccccaggg
    25381 aaataaaaat gcagtttcga gatcaaacaa aatgaagagg atggcgatga ggtaataacg
    25441 cacatcgaat ttcatacgag catcttcgaa ggcctcaaag ccgcactcgt atggagaaac
    25501 tttttctgaa tcaggtttgg agggagctaa aactttaccg agaataatag gagccaatcc
    25561 cacaccgata ccgacaagta tgaacaacag cactgggaag taatttgaaa ggttcaactt
    25621 ctatcctgcg tcagttttat tccaaaaaca caaaaaaatt atgtacgatt tcaataaatg
    25681 aactcttgat ttacgacaaa gactccaaga attcccgaac aaaactaatc catattggtg
    25741 ccgacggcga gactcgaact cgcacagcct aagccactac cccctcaaga tagcgtgtct
    25801 accaatttca ccacgtcggc atcttggaaa agccctacaa caacccacga ttctactgca
    25861 ttgcaccctt tttaatgggt cacaattact ttggtacgac cggctttgct ggctctgtac
    25921 cccctgccgc tgcaggtgct ggaacagatt gattaccctg ctgagcaggc gctactgctg
    25981 gcagggttga gtttgataaa acccctgact caacaggctt tttactaccc aaccaggtaa
    26041 taccaagcgt gctcacaaaa aagataacag caaacacagc ggtagtgcgc gatagaaaat
    26101 tggcagaacc tgaagcacca aaaacacttc ccgaggaccc agatccaaaa gcagcaccca
    26161 tatcagcgcc cttaccctgt tggattagta ccaaaccaat aatggccaag gctgagacca
    26221 cctgcaacac aacaaataaa gttttaagcc actccatatg atttctccat aaataaatca
    26281 gttagttcat caagcaaact atgctcggca aattgctaaa aattcttggg catccaagga
    26341 tgctccgcct actaaaccgc catctatatc gggcattgca aataactcgg ttgcattatc
    26401 tgcgcgcacg ctacccccgt aaataatccc aatatgcgct gccacgtcat gatcaaactc
    26461 tgctaactgt agacgaatag cccgatgcat atcttgagcc atttgagcgc tcgcaacttt
    26521 acctgtgccg atagcccaaa taggttcata ggcaattaaa caatcaacca agcgatcttg
    26581 taaggtactg acttggctag caacttgacg cctcacggcc tcaatcgcac gcccagaatt
    26641 tcgttcatca gcagtctcgc ctacacaaat aataggtgta atctcatgat ccaaagcggc
    26701 tcctgccttc agtgcaatcg tttcatttgc ttcgccgtac tgcatgcgcc gttcggaatg
    26761 accaacgatt gcaaaacgga cacccatctc ttgaagcatt accgcactga cctcacccgt
    26821 atatgcacct gactcatgcg cagatacatc ctgagcccca agcagaagct gactacttgc
    26881 taataattgt gagcactgat gtaaataagg gaatggaaca cacacaccaa aacgacgacc
    26941 cgcaggcata ccgcattgca taccttcgcc aaccgattgc atccatgctt gattgataga
    27001 aagactgcca ttcatcttcc aattaccgat gacagtgagt gcgcgcatgg ttgatccgaa
    27061 ttacgcagtc agaataattt tcccgacatg ctcactagac tccatcaaac gatgggcatc
    27121 agcagcctgt tccagggaaa atgttttata aattactggc tttaatgcgc ccgaattgag
    27181 caagggccat acatgctcat aaagctcttt ggcaatctga cgcttgaatg acactgggcg
    27241 tggccttagt gttgaacctg tcacagtcaa acgacggcgc aatatctgac ctgtattgac
    27301 ctctgatttg gagccaccca taatcgcaat caccacgata cgtccatcat ctgcaagaca
    27361 gtcaatttct cgttgcaagt aagtacctgt gaccatatct aaaatcacat caacaccttt
    27421 gccattggtg accttcttaa cctcttccac aaagtcttga gttttatagt tgatcgccac
    27481 atctgcgccg agtttgatac aagcagcgca tttttcatcg ctaccagccg ttacaaaaac
    27541 acggtgaccc atggccttag ccagcatgat ggcggtcaca ccaatcccgc ttgagccacc
    27601 ctgaactaat aaagtttctc cagcgctaag ctggccacgc tgaaagacat tgctccacac
    27661 ggtaaagaaa gtttctggaa gtgatgctgc ctcaacatca ctaaagccgt ttgggtaagg
    27721 taagcattgt gcgattggtg caatgcaaag atcggcatag ccaccaccct gaaccaatgc
    27781 acaaacccga tcgcctatct ttaaacccag actgttatcg ctgtgtgcta aatcgccacc
    27841 aataacttct ccggcaacct ccaagccagg aatatcggat gcgccagcag gaacgggata
    27901 gtgccctttt cgttgcaaaa catcaggacg attaatgcct gatgccttca cccgaattaa
    27961 tatctctccc gagcctgaag caggtactac cggatccggt cgttctgcct tgaccaacat
    28021 ctccggtgca ccaaattccc gaatctccat cacgcgcatt gcaaatctcc tgcgtttagg
    28081 tggtctcgtt ggagcttgca ctcatttctg aattacttgc accgccatct tgagctaaga
    28141 gcgccttcat agaaagtcgt agacggccac gttcatcggc agccaataac ttaactcgca
    28201 caatctgacc ttcttgcaaa tggtccttga cgtctttgac acgctcattg gagatctcgg
    28261 agatatgcag gagaccgtcc ttaccaggca ggatattgac taatgcgcca aactcgagaa
    28321 gcttaacaac tgggccttcg tatattttgc caacttctgc ttcagcggta atgccctcaa
    28381 tgcgtgactt tgcttccgcc attccttcag cactcgtaga ggcaattgtt acggtgccat
    28441 catctttaat atcaatgctg caaccggtct ctttagtcaa agcctgaatc gttgcaccac
    28501 ctttaccaat cacctcacgg atcttgtctg gatgaatctt aaagctcacc atacgnnnnn
    28561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcagtaat accatttgcg gtaccagcaa
    28681 ccttaaagtc catatcgccc aagtgatcct catcacccaa gatgtcggtt aatactgcaa
    28741 agcgattgcc atccaaaatc agacccattg cgacaccagc aacgtgtgct ttaacaggca
    28801 cgcctgcgtc catcaatgct aagcaaccac cacataccga tgccatggaa gacgaaccat
    28861 tggattcagt aatctctgag accaaacgaa tactgtaagc aaaatcttct aagctcggca
    28921 acactggaat caaggcacgc tttgctaaac gaccatgacc aatctcacga cgctttggcg
    28981 tacctacgcg accagtctcg ccagttgcga atggaggcat gttgtagtgg aacataaagc
    29041 gatcacgata ctcaccctct agtgcatcaa taatttgctc atcgcgtgcg gtacccaaag
    29101 tagcaacaac cagcgcttgg gtctcaccac gagtaaagag tgcagagcca tgtgtgcggg
    29161 gcaatacgcc attccgaatc tcaatgggac gaacagtacg tgtatcgcgg ccatcaatcc
    29221 gtggctcgcc attcaaaatc tgactacgaa cgatcttcgc ttcgatctca aataaaagat
    29281 tgcctacctc gacttcgtcg acctcaccat ccgcagacaa ttgggtcatc acattagcaa
    29341 ctacttcttt gatcttagta gaacgggctt gcttctgacg aatttgatac gcctcacgca
    29401 gtgggccttc agccaagctt tgtaatttag caatcagtcc ctcattttta gcagcaggct
    29461 tccaatccca ctctggctta ccggcctcac ggactagttc ttgaatagca ttaatagcaa
    29521 tttgagcttg ctcatggcca tacacgacgg cgcccaacat cacagactca gataactgct
    29581 gagcctcaga ctcaaccatc aatacggctg cttgagtgcc tgctactatt agatctagct
    29641 cgctagtggt ttgctctgag cgatttggat tcaataagta ttgaccatct cggtaaccaa
    29701 cacgagcagc gcccattggg ccattaaatg ggatacctga aatagcgagt gcagcagagg
    29761 acgcgatcaa agcaggaata tcggcgggca catctggatt gatcgacatt acatgaacaa
    29821 ccacctgaac ttcgttatag aagccttctg gaaataatgg acgaatagga cgatcgatta
    29881 agcgagaggt taaggtctcg ccttcagatg ggcgtccttc acggcgaaag aatccacctg
    29941 ggatttttcc tgcggaatag gtcttctcaa tgtaatcaac ggtcaaaggg aaaaagtctt
    30001 gaccagcctt agcggtcttt gcacccacta cggttgctaa gacaaccgtg tcatccatat
    30061 tgagcatgac agcgccgctc gattggcgag caatttcgcc agtttccatg gtgacagtat
    30121 gctgacccca ttggaaagtt tttactactt tattaaatat tgacatcgtt ttctccaaat
    30181 aaatacttcg cggataagca gcgccgcgat atcactggag tgattgatag aaaaacccaa
    30241 ttgggatgcc attccaatga acctcttcct caagaagttc attggaatga cacgatccct
    30301 agaagccggt catctagaac cactcaaagt aaagccccgc ccatttggca agagccctac
    30361 ttcattgcaa tgctgacaaa aacagtcgga agttacttac gtaagccgag tttgtcgatc
    30421 agcgcgcgat aacggtccag atctttgccc ttgaggtaat ccaagagacg gcggcggcgc
    30481 gagaccatct tcaacaagcc acgacggctg tgatgatctt ttgcgtttgc cttgaaatgg
    30541 ggggtaagtt cgttgatacg agcggttaac aaagccactt gaacttctgg gctacccgta
    30601 tcgtttgcgc cacgcgcgtt ttgctggacg atttccgcct tattaatgtc tgcaactgcc
    30661 atattcatac tccatacgtg cgaacactcg agcttgcacc cgatatgccg tgttaataaa
    30721 aatgctttat aaatcaaagc ctcagcattc taacagaccc aaccctcttt ttatgagggc
    30781 atgggctagg cattgtataa atctatataa atcaataact tataaattta tggggccatt
    30841 tgcgcgttga gcttaatcgc gttgggatca tgcaatttgt tcaaagccgc caaataggct
    30901 ttggccgacg ctgcaatgat gtctggatcc atgccgacgc cattcacaat ccgtccgcct
    30961 tttgagagac gcacagtgac ctccccttgt gattccgtac ccgatgtaat cgcattaacg
    31021 gaatacaaca ataactcggc gccactcttc gcctcggtct cgatcgcatg caaagtagca
    31081 tcgacaggac cattgccctc agactccgaa tgcacatcct taccgcccat cttgaacacg
    31141 actttcgctg ttggcttctc accggtctca gaatgttggt ttaaggatac aaaactaaag
    31201 tactcaccct ggtcggttat ggatgaatct gacatcaaag aggtaatgtc ttcatcaaag
    31261 atctctgctt tttgatcagc caatgcctta aagcgtgcga aggcatcgtt aagctcactc
    31321 tcggagtcca agacaatgcc taactcctga atacgttgct taaaggcatt ccgcccagat
    31381 aacttaccaa gtacgatccg attggttgcc caacccacat cctcggcacg cataatctcg
    31441 taggtatccc gtgctttcaa aacaccatct tgatgaatcc cagaggcatg agcaaatgcg
    31501 ttcgcaccga caactgcttt attaggctgc actacaaaac cagtaatttg tgaaaccaat
    31561 ttagatgccg gcacaatttg tttcgtatca atgcctaact ctaatttaaa gaaatccttg
    31621 cgggtacgaa ctgccatcac aatttcctct aaagaggtat taccagcacg ctcgccaaga
    31681 ccattgatag tgcactccac ttgacgggca cccccaatat gaactcctgc caaggaattc
    31741 gctaccccca tacctaaatc attatggcaa tgcacagacc aaatcgcctt atctgagttt
    31801 ggtatccgcg tacgcaaagt tttgatgaac tcgccataca gctcaggaac tgcatagccc
    31861 acggtatcag gaacattgat ggtcgttgca ccctcagcga ttactgactc caaaactcgg
    31921 cataagaaat ccatatctga gcgatagccg tcttcgggag aaaactcgat atcacctgcc
    31981 aaatttctag caaaacgaat tgatttcttc gcctgctcga atacctgatc aggggtcatt
    32041 cgtaatttct tttccatatg taaggcactg gttgccagaa acacatgaat ccgtttagct
    32101 tgagcaggct ttagggcatc ggctgcgcgc gtgatatcag cctcattagc tcgagctaag
    32161 gagcaaacaa tagagtcttt cacagcggcg gcaactgcgc taatagctgc aaagtcacct
    32221 tcggaactcg ctgcaaaacc agcttcaatc acatccacgc gcaagcgctc aagctgacgt
    32281 gcaatccgca ctttttcgtc acgagtcatg gatgcaccgg gtgattgctc accatctcgc
    32341 aaggtggtat caaaaataat taatttgtcg cccatagtat ttcctcttta actgcacagt
    32401 tccacaaaca aaaaccccag ctgtttggct ggggctggtt ggttagctag taactacgaa
    32461 ttcattttgc taagcgcgac ccgccccgag cctaaggccc agtgccagta gaagtagtaa
    32521 aagcgcgctt ggtttcaata acatgactta actataccgc atttaatctg atttgtggat
    32581 ggctgtggtt tccccaccaa ttcgacgtcc tgttgccctc tcccaaagcc acagcacgta
    32641 acccgataag gcgtacacca cgaatacacc aaataaggtg aggggtggat ttgtagatat
    32701 caggacaaag cctagaatca ctaacaccat aacccaaaaa gggacgcggt agcgaacatc
    32761 caaagcctta ccgctataga aacgtgcatt cgataccatc gtgagtcccg catagatggt
    32821 gatgaaaaag gtcacgatcg gaattgctga atccttaacc ggaatcttat tgtcatcggc
    32881 caaccaaata aaaccggcaa ttaaagcagc ggcagccggg ctgggaaggc cctgaaaaaa
    32941 tttcttatcg accacaccaa tgttggcatt aaaacgggct aggcgtaaag cagctccagc
    33001 acaatacgta aaggcagcca accagcccca cttaccaaga tcttttaaga cccactcata
    33061 cgccaccaag gcgggggcta ctccaaaaga aaccatatca gctaatgagt cgtattgctc
    33121 accaaaggca ctttgcgtat tggtcatgcg ggcaatgcgt ccatccatac cgtctaagac
    33181 caaggaagca aaaatagcaa ttgctgcgac ctcaaaacga tggttcatgg cgtttacgat
    33241 tgcaaagaac ccacagaata gtgccgccgt cgtaaatgca ttaggcaata aataaatact
    33301 tttactgcgc ggtcttgcag gcaaggagat gggatcctca tcaagcgact cgtcattacc
    33361 gtcccaatca tcgcgtcgta cgtttctact ccttaaaaaa cggagacgat cttgacgaaa
    33421 tttacggcgt ggcaacatca attaatccaa gcctggtagg cgggctaaag cagtattcgt
    33481 tgcaaatact ttttcaccaa cactgaccaa tggctctgcc tctaagggta aataaacatc
    33541 tacccgtgaa ccaaaccgaa taaagccgta acgctctccc cgcttaactc gatcattgac
    33601 gtggatgtag caaagaatac gacgcgctac taaaccagca atttgaacca gggttactat
    33661 tttgccgttc gcatcaatca ccaccgcatt gcgctcgttc tcagtcgaag ctttatcaat
    33721 actggcatta aagaacttac caggaaagta agttacctgt ttaatcaggc cgctcacagc
    33781 acagcgattg gaatgcacat tgaatacatt cataaaaaca ctaatcttca acgcctcacg
    33841 attagcataa ggatcgtgtg ctttctcaac cactacgatc ctaccatcgg ctggagagat
    33901 aaccaggtct ttacccaagg gaacaatgcg ttgcggatct cgaaagaact ggagggtgaa
    33961 gacaaaaaat agccagaggg gccaagacca aaagagtcca ccaatgcgat gcacaataaa
    34021 tgcaagcacc ccgatcacta ccaaatacaa ccatccctct ttagcgatga tggggtgtgg
    34081 atacatcata agttgatcta ctttctctaa attagttctt ggattgatct actaatttgt
    34141 tctttgcaat ccaaggcatc atggcacgga gttttgcgcc taccacctcg atctcatggt
    34201 cagcggttaa gcgacgacgt gacatgagag ttggagcgcc agcccgattt tcaag
//
LOCUS       DDPQ01000047            9762 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_31872,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000047 DDPQ01000000
VERSION     DDPQ01000047.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_31872"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2059..2117
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2300..2343
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9452..9497
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2506 a         2352 c         2238 g         2517 t
ORIGIN      
        1 tggctcattg cacgccgtaa gaaggtggaa ggcatgcaat gggccagctt aatcttgatc
       61 gtgttctttg gtggcttaac cattctcctg aaagataaaa cctttattca gtggaagcct
      121 acggtcttgt attggctatt tgctgcggtc ttgttcttta gtgcgcagtt cttcaagaag
      181 aattggattg agagcctcat gggtaagcaa ataacgctca agcccgatag tcctaaatca
      241 ctttggctca aactcaatca tgcttgggca ctcttcttct ttttcatggg cgtacttaac
      301 atctatgttg cctatacttt ctctgaagat atttgggtga acttcaaact atttggtggc
      361 attggtttgc tgattgcctt tgtgattgcc caaggcctat ggttaagtcg tcatatggaa
      421 gcagagcaat catgagcaag aatcaagaac gtattgtttt gtttgaagcc gccctacagc
      481 agcaactgca accatcgcat cttgcgattg aggatgaaag tcatttgcat gcaggtcatg
      541 ctggggcagc aagtggcggt ggtcattttc ggattacact caccgcacct gcatttgctg
      601 gactcaatcg agttgcccgt caccgcttgg tgtatgaggc gctgaaccct tacatcccca
      661 cagaaatcca cgccctcgca attcatgctc ttgcaccaaa tgagtcataa ttctgtttta
      721 gtcctttaat tcaattcact atctctcatg cttaattcca agaaaatcct tgtagccctc
      781 tcatttgcct ttgttaccac tgcggtctca gctcaaaacg ccgcaatcgt caatggcaag
      841 gccattccaa aagcacagct cgatcgctta attaagaact ctggtcaatc ggctacggat
      901 cctaagatac gtgaacaagg tcgtgagtta ttaataaccc gtgagcttgt catacaggag
      961 tcagataagc gtggccttac tcagaaagag gaagtgaaag atcaaatgga acaagctcgc
     1021 ttaggcgtct tggtctctgc tttgtttgat gattacattg ataagggtgg cattactgag
     1081 gatgattaca aagcagccta tgaatcagtc aagagccaat ttggtggtaa agaatataaa
     1141 gtccgtcata tcctagttga gaaagaatct gaggccaaag ctttgattgc taagattaag
     1201 gcgggtgcca agtttgaaga tctagccaaa gcgaactcta aagatccagg ctctgctcct
     1261 aacggtggtg atctggattg ggttagtgat aaggccttgg taccagagtt ttctaaagtc
     1321 atggttacct tgaagaaagg tcagatgacc gatcagcccg tgaagactca atttggttgg
     1381 cacattatta agcttgagga tgttcgggat gtgaaagtac ccaccatggc tgagatcaaa
     1441 gatcagctta agcaaatgat gttgcaagac caagcctggc aaaaggcaaa gtttgctgag
     1501 atgatgaaaa agatgcgtga gaaagcgcgt attgagtaat atgattttgc acaccatgct
     1561 tcgggtgggc gatattgccc gatccgttga tttttataca cgcgtcttag gtatgaacct
     1621 attgcgcacc actaatcgtg aagagcaaaa atactctctc gcatttgtgg gctttggtcg
     1681 tggcaacgaa gatggtcaag cagaaattga gcttacctat aactatgggg tgagtcagta
     1741 tgagcatggc ggtgcttatg gtcatattgc tcttgctgtg cccgatgtgt ataaagcctg
     1801 tgaggatatt gcagcagccg gtggaaatat tacccgccct gctggccctg ttcagggtgg
     1861 caagacctta attgcgtttg taaccgaccc tgacggatac aagatcgaac tgatccaaca
     1921 ttagttcacg atgagtcaag tacctgaact cgagattcgg gtcatacgaa gtcttagtag
     1981 cattgcacca tctgactggc agcgcgtttt gcctaaggat gccggcccct tcttgcacta
     2041 ttccttttta agcctcttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnntta aagacccatt cttatggtga atacgtattt gattggtctt
     2161 gggcagaggc ttatgcgcaa caaggtctct cctattaccc caaggcctta tcggccatac
     2221 cctttacgcc ggcaaccggc tcgcgtctct tagcgcgtac tgctaaccat caagccgcct
     2281 tagtaagcgg tcttgtacan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnntaaccga aatcggcttt atgcgacgtg agtctgtgca gttccattgg cacaatcaga
     2401 actatgctga cttcgatcag ttcctagcca cgctcacaat gaaacgtcgt aagaacatta
     2461 agcgagagcg caaacaggta cacgatgccg ggattacgtt tcgtcacata cccggttcac
     2521 agatcagcgg ggacgaagta ctcatgttct atcgctgtta tgccaatacc taccgaacgc
     2581 actattccac cccttactta aaccaattgt tcttccaaaa atgggttcag gctatgccag
     2641 actcattgca cttcattatg gcgatccgcg atgggcaaac tattgcgtct gcattattag
     2701 tcattgaccg agatggtcat aaggcttatg ggcgctactg gggctgttta gaacagcatc
     2761 cttgtttaca ttttgagacc gctttttatc aagcgattga gttctgtatt aaagagcata
     2821 tacagctact agaaggaggt gctcaagggg agcataagat ggcaagaggc tttatgccaa
     2881 gcactgttag ctcctatcat tatctggttg accaacgctt tgccgatgcg gtcggcaggt
     2941 tcttagagcg ggaacgtatg ggaattggtc aatatctgga tgagctggct gagcatagcc
     3001 ccttgcgcca gagcagtcat ggcctagagc tcggtaagaa taatgagaat gcgctaaagt
     3061 agcttatgtc taataatcac tccaaccctt ctgctggtgc caactcaatc ctcattggcg
     3121 atgcagactc cgactcaccc tgcattggta tttgctctac tctctttgat gagatctgca
     3181 agggctgtgg gcgcactgcc ctagaggtca gtaattgggt atttatgagt ctagaggaga
     3241 agcaggcggt ctggactcgg atacaggcag agggtacagc gatgcgcttt acccgcgagc
     3301 aaaagaccta acctgtaagc gcttgactac gtaaatactc ttcgtaggtt cccgagtagt
     3361 cattaatcgt accgtcgttt ttaatctcta agatacgatt tgcgagtgct gaaacaaact
     3421 cacggtcgtg cgatacaaag attagggttc cctcaaactt ctctaaagaa atctgtaagc
     3481 tctcaatcga ttccatatcc atatggttgg tcggttcgtc catagccaag acattatgtt
     3541 tttggagcat taatttgccc cagatcagtc gacccttctc gccacccgat actaccttga
     3601 ccgatttacc aatctcatcg cccgagaaca ataaacggcc caagatacct cgcactgctt
     3661 gatcatcatc gcctggctta cgccactggg tcatccattc aaacaaggtt tcttctttag
     3721 gaaaacactc gctcgtatct tgtggcatca cacccacatt ggcgttctct gcccacttca
     3781 catcaccgct atcggctgga ataccatcaa agcgtttgct aagaatcgtt ttaagcaagg
     3841 tggtcttacc ggcaccgttt tgtccaatga tggcaacctt ctctcccgga cgaattgcga
     3901 tcttgaagtt cttgaagatc acacgatcaa attttttggt cagtgcattg cactccaccg
     3961 ccatattgtg taatttcttt tcggtttcaa accgaataaa tgggttctgg cgcgatgagg
     4021 gtttaatctc agccacctca attttctcga gctgacgttg acgcgaggtt gcttgtctcg
     4081 ctttagatgc gttggctgag aagcgactta cgaaggcttg cagttcagca atcttctctt
     4141 tggccttcgc attcgatgcc agttgttggg ctcgtgcttg ggtagaagcc aacatatagg
     4201 agtcgtaatt acctgggtag accttaaggg tcccaaagtc catatcagcc atatgcgtac
     4261 acacctcgtt taagaagtgt cgatcatgag aaataatgat catggtgctg ttacgctgat
     4321 tcagaacatc ctctaaccag tggatggagt ggatatctaa gttattggta ggctcatcaa
     4381 gcagcaaaac atcaggatct gagaacaagg cctgcgccaa cagaaccctc agtttccagc
     4441 ccggagcaat attactcatc gggccattgt gttcctctaa cggaatccca atacccagta
     4501 ataactcacc ggcttttgct tctgctgtgt aaccaccata ctccgcatat ttagcttcga
     4561 gctcggcagc acgcatatag tcttcgtcgt ttgcatcagg gttcgcataa attgcatcac
     4621 gctcagcggc agcttgccac atctctgcat gccccatcat caccacatct agcacccgca
     4681 catcttcata cgcaaattga tcttggcgca atttacccaa acgaatatta ggctctaggc
     4741 tgacattgcc cgcagagggc tctaactcgc cacctaagat tttcatgaaa gtggatttac
     4801 cacagccatt ggcgccaatc aggccgtagc gattgcctcc accaaatttg acggagatgt
     4861 tctcaaacag gggctttgcc ccaaactgca tagtgatatt ggatgcgttt aacacaacga
     4921 ctttctataa caaaagatga atcgggaatg ccgggtggca aagctcacta ttttaacggg
     4981 cttctgaaac gagggatgtt agtaggctgc ggctagacca tcgcgccggc tatcgctggc
     5041 cgcgacatag ccgtcggcta tctggtcgct aaggcgccag ataaactggc ctgaaccaaa
     5101 gtccatatag ggatcttcta ccttttttag gatatgcccc ttttctacta ggccctggac
     5161 ggtctctggg ttcatattgg cctcaacatc taggctaaag tcgcgattta ctttccagcg
     5221 aggtgcatca caagctgcct gaggctgctg tttatagacc aacattctga gtagggtttg
     5281 cagatggcct tgcggttgca tatcgccgcc catcacccca aagctcattt gaggaactgt
     5341 tttgccgttc acctctttgg tcaagaaggc cggaataatc gtatgaaagg gtcgcttatt
     5401 accagccacc acattcggag aatttggatc ggacgagaac ccataaccgc ggttctgaaa
     5461 actaattccc caattgggct ccactactcc agaaccaaag cccatgtaat tactctgaat
     5521 gaatgagatc attcggcctt cctcatcggc tgcgctgaga taaatggtgc cgccagtatt
     5581 gggcatccca aaggagaact gagtcgcttt ctgttgatca attaattggg ctcttttggc
     5641 taagtaatcc gtgttgagca tttgcgcagg gctaacttcc atcgagcgcg gatcggccac
     5701 atagcgataa acgtctgcaa aggcaagttt catggcttca atttgcagat gctgactctc
     5761 aacaccatcc acaggatatt tagccaaatc cagttgctct aggataccca tagcaattaa
     5821 ggcaccgata ccttgaccac ttggaggaat ttcgtgcaca tcatagccct gcactttggt
     5881 tgagatggta ccgacccaat caggtttgta attagctaaa tcactggcag tcatcgccgc
     5941 accatgttct tttgcaaacg cagtaatggc ccgtgcaatg tcgccctcat aaaaatctcg
     6001 caggcctact tgcgctaatc gtttgagagc ttttgctgcc tgtggaaaat aaaacatctc
     6061 accggttgca ggcgccctac ccttgggcat aaaggcttgt gcaaaaccgg gttgtgaggc
     6121 taactcgccg gtagccgctg cccatttgtg ggcaacaatt ggggctagag cgtgccctct
     6181 ctctgcaatc tcaatcgcgg gttggagtag atccgcaatg ggtaatgagc caaagcgctc
     6241 atgcaaggtt tgccagccag ctaatgcacc tggcaccgtt acgctatccc agccccgcat
     6301 aggacgtttt gcgagaccat tactctccct gccatacttg gcggcaaagt aatcgaggct
     6361 ccatgctgcg ggtgagactc ctgaggagtt cagtccatgc aattgctcac catcccacac
     6421 gattgcaaag caatcacttc caagaccatt ggatacgggc tccacaatca ttagggctgc
     6481 agcggcagca atcgccgcat caatcgcatt accaccctga tgcataatct tcaagcccgc
     6541 ttgggctgca taagggtgcg aggttgagac aatattgcgc cccatcaccg gcatgcggat
     6601 ggtgggatag ggattattcc aattaaatga cattgctatt ccttaagaac gggttcgcgt
     6661 caatcgtttc tgaatgactg gaactcggat cactaataat aggaagatta tcccagcata
     6721 gatatagacc acctcaaaat cattctttcc ggccttatgc cacagatagt gcaagactcc
     6781 gaggatggca attagataaa ttaggcggtg caataaagac cacttgcgcc ccaaccaacg
     6841 catagccatc tgatttgagc tgatagccaa gaggctcata cagacaaagc ccagaaatcc
     6901 catagtgatg aatggacgct caatcacatc atcaatcatt gccttggcat caaggttgtg
     6961 atccaaccaa taccaaatgg tgaagtgcaa aaatgcatag aagaaggtaa agagccccag
     7021 catgcgtcgt aactgtatcc acccactcca ccccgacatc caacgcattg ggctcatgct
     7081 taaggtgatg cacaagaagg taatggtcca agtgccagtc gatcgggtaa tgaactctac
     7141 tgggttggct gtgagatcac caaggtaacc caagaccaat aaacgcccta gcggtattag
     7201 cgccagtgca aagacaaaat acttgatgga ggtaatacct ggcattgcct agtaaaactt
     7261 ttttagatcc atgccggcgt agagttttgc cacatactcc ccataaccat taaagggctc
     7321 ggtcttaatc ttgggtgcaa agatgccacg accatcaatc cgtcgctcgg tcgcttggct
     7381 ccaacggggg tgactcacgc ttggatttac attggagtaa aaaccatatt cgcgcggtgc
     7441 agacatgctc caactcgtag gcggttgttt ttcggtaaag cggaccgtca ctaatgactt
     7501 agcactctta aagccatact tccagggcac cactaagcgc actggcgcac cattctgctt
     7561 gggcaaaatc tctccatata agcctaaggt taagagtgtc agagggttca tagcctcatc
     7621 catgcgtaag ccctctcgat agggccagtc caatacgggt ctacgtaagc ccggcatttg
     7681 ttttggatca gcaagagtaa tgaactccat gtatttggca gagcccaagg gttctacttt
     7741 attaatcaga tgcgagagtg catagccgtt ccaaggaatc accattgacc aaccctctac
     7801 acaacgcatg cgatagatgc gctcctccat cggagctaat ttgagcaagg catcaatatc
     7861 gagagttaag ggctttttaa ctaagccctc aatgctgatt gcccaaggtc gggtttgtaa
     7921 acgctcagcg tatttggcgg gctcatcctt gtccatacca aactcataga agttgttata
     7981 gctggttact tcttcatatg aggtggcagt ctctttggtg gagtattgtt cattgaactt
     8041 agctgctaac ttctctttag caattggggt tttagcgaac gcctcacgct ccaaccatga
     8101 gccaagaccc atcgccatcg atcccatcgc tgcggtcttt agccactcac ggcgctgttc
     8161 aaatactctg cgtggggtga catcgctact ttgtatgcgt ctatctttaa atatcatcga
     8221 tcaaccttta attgaaatgg tgtgaaattg gtattggtat cgtaataatc ttcattctct
     8281 ttacgcttca gatagccaat aatccagtag gtaaaggggg ttgctaaaac ctcccacgag
     8341 gtcttcaata tatattgcgc tattgctacc tgaataattt cagcagtcgg ccagacagca
     8401 taaaaggcaa tcatgtagaa cagagatgag tccaccaact ctcccgttgc ggttgagcca
     8461 atggtccgag cccacaaatg cttgccttgg gtccagacct tcatcttggc taagacatag
     8521 ctattaacaa agctaccgca ccaaaaggca atcatcgatc ccaatgcaat tcgccacgaa
     8581 ttaccaaata ctgtttctag gccttgctga taattagcca tgtatgaccc aggcgctact
     8641 ggtaaatgaa ttaccacctg tgccatgatg gctgcaaagc ctaaggctgc aaatccagcc
     8701 caaacggtgc gccgatcata tgcataacca tagacctcgg tgagcacatc gctaatgaga
     8761 taggagatcg ggaaaaacaa gataccggcc ccaaacacca ccaagccaaa atatggcaga
     8821 tcgagggtcg cagccttacc cgcaccaatg aagttagagc ataggatcac cgccacaaat
     8881 gcggccaaga tcaggtcgta atagcggtat tgccggcgca tagttagcca agggcccttg
     8941 tggtgactgc tttcctgtta aatcagtgaa tatttcatag aatagcggta ttcagtcttt
     9001 ttatccgaat tcatctcaaa tccagaaaaa taatgagcaa accaagcttt cactgggaag
     9061 atccccttct cctccaagac caactaagta ccgaagagcg catgatcgct gatgccgcta
     9121 gagaatatgc ccagggcaag ctcgcaccgc gggtccaaga agcctttcgt gcggagagta
     9181 ccgatccatc catcttccga gaaatgggtg agctggggct tctggggatt actatccccg
     9241 agcaatacgg tggcgctaat ttgaactatg tctcgtatgg cttgatcgcg cgtgagattg
     9301 agcgggtaga ttcgggttat cgctccatga tgagtgtgca atcttccttg gtcatggttc
     9361 caattaatga gtttggtact gaagcccaga aacaaaaata tctccctaag ttagccactg
     9421 gtgagtggat tggttgcttt ggcctgactg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnncag gggggttctc gctaacgggc tccaaaatgt ggatctcaaa
     9541 ctcaccaatt gccgatgtct tcgtggtctg ggctaaaaat gatgcggatc aaattcgggg
     9601 cttcatcctt gaaaaaggga tgaaaggttt aagtgctccc aaaattacgg gtaagcaagg
     9661 attacgcgct tctattaccg gcgagattgt gatggacgag gtgttcgtac ctgcggagaa
     9721 cgagttcccc gaggtcactg gcctaaaggg tccgttcact tg
//
LOCUS       DDPQ01000048            3782 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_12287,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000048 DDPQ01000000
VERSION     DDPQ01000048.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_12287"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    122..216
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1103..1239
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3112..3161
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          873 a          730 c          911 g          986 t
ORIGIN      
        1 ttgatgatgt gtctctacaa gttaaagagg gtgaattctt tggtctgttg ggtccaaatg
       61 gggctggtaa aactaccctg atctccatac tggctggcct ttgtcgtcca gatcagggag
      121 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagga tgttgggcgt ggttccccaa
      241 gagctcgtgt ttgatccatt tttcacggta cgcgagacct tacaatttca gtcgggttat
      301 tttgggattt tgcataacga tgattggatc gatgagatca tggccaatct agatctcacg
      361 actaaggcga atagtaatat gcgatctttg tcagggggca tgaaacgtcg agtcttggtt
      421 gctcaagcat tggtccaccg tcccccagtc attgtcttgg atgaacctac tgccggagtc
      481 gatgttgaac ttcgtcaatc gctctggaaa tttattagcc gtcttaatca agatggccac
      541 actattgtat taaccacaca ttacctagaa gaggctgaga gtttatgtgg tcgtattgcc
      601 atgttgaagt ctgggcaggt ggtggcgttg gataccacag caaacctact cgcgcgttat
      661 ggaaagactc gcggggctga tggacaagag accgatttgg aagatgtatt tatgcaaatc
      721 atggcagggg atcaacaata atgcgacctg cattggagta cggtagcggt ttttttaccc
      781 tattgcgcaa agagattaaa cgcttctata aggttgcctt tcagaccgtt gctgcacctg
      841 ttttaacggc cgttctatat ctcatgatct ttggtcatgt attagagggt cggctggtgg
      901 tatatgacaa attaagttac accgcatttc tgataccggg tttagtgatg atgagcattt
      961 tgcaaaatgc ctttgccaat acttcttcat ctttaattca gtcaaagatc acaggcaatt
     1021 tagtgtttat tctcttggcc ccattaacgc accttgaatt ttactcagcc tatgtattag
     1081 cggcagtatt tcgagggatt gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tcttaatcat gcctgcgacg
     1261 atgctctcag gggtgtttta ctcgatacat tccctaccaa cgatctggca agtcatttcg
     1321 cacttcaacc ccttctttta tatgattgac ggctttcgtt acggtttttt tggagtctcg
     1381 gatgtctcgc cttggatgag cctaggagtg gttggaggat tttttgtgat tgtctcggcc
     1441 ctggctttgc gtttactaca atcaggctat aaattacgct attaaaacca ttgaatgagg
     1501 aaagcagatg ttccccacac cagagcagat tgaaggctat atcaaggaca aacttccttg
     1561 cacgcatcta atggtggagg gagatggcca gcatttttat gccacgattg tgagccctga
     1621 ctttgctggt aagcgcttgg tacaacggca tcaaatggtc tatgaagctt taggagatcg
     1681 catgaaagca gaaattcatg ccctatcgat taaggcgttt acccccgatg agtttgctgc
     1741 tcaataagca ctcattcaga atcaatcaat taaaacggat tcatggataa attagtaatg
     1801 acgggtggct caccccttca tggcgaggta cagattgcag gcgcaaaaaa tgctgctttg
     1861 cctattttgt gcgcttgctt attaagttca gacacaatcg agttgcataa tgtgcccgat
     1921 ttacaagacg tacgtaccat gctgaagtta ttgcagcaaa tgggagtgat ggttgacttc
     1981 ccggacccca atgatcgcag tcatttgtgt ctcaacgctg gcaagatcat tagtccagaa
     2041 gcgccctatg agttagttaa aactatgcgc gcatcaattt tggtattggg cccattgctc
     2101 gcacggatgg gtctagcgac ggtgtcgttg ccaggcggtt gtgcaattgg cgcacgacct
     2161 gtcgatcagc atattaaggg ccttaaggcg atgggcgctg gtatccagat tcgtaaaggg
     2221 tttattgttg ccaagattgc ggaaggtaag ccgcgcttgc agggcaatgc gattctgacc
     2281 gacatgatta cggttactgg tacagaaaat ttgctcatgg cagcttgttt agccgaagga
     2341 actacggtgc tagaaaatgc tgcacgtgag cccgaagtgt cggacttagc agagctctta
     2401 gtaaaaatgg gagcgaagat ttctggaatt ggtagcgacc gcttggtgat tgagggtgta
     2461 tccgctttac acggtgcaag tcactccatc atcccagatc gaattgagac cggaacattt
     2521 ttatgtgccg tggctgcgac cggcggtgag atcacgctta agaactgtcg acccgatacc
     2581 ttggatgtgg tcttagtgaa gttaaaagaa gcgggcctta agttgaagaa gggcaaggat
     2641 tggataacgg ctagcatggt aggtcgccct aaaccggtta gttttagaac ctcagaatac
     2701 cccgcctttc caaccgatat gcaggctcag ttcatggctt tgaacgctat tgccagcgga
     2761 aattcccgga ttaccgagac tatttttgag aaccgcttta tgcacgttca agagctcaat
     2821 cgtttgggtg ccgatatctc gattgagggc aatacagcca ttgtcgaggg ggtggataag
     2881 ctctcggggg caaccgttat ggcaaccgat ttgcgtgcct cagcaagtct tgtcattgca
     2941 ggcttagcgg ctcaagggga gacccaggtt gatcgcatct accatcttga tcggggctat
     3001 gaccggatgg aacaaaaatt gacccaaatt ggcgcaaaca tccgccgcat gaaataatta
     3061 acgcattatg ttgacactcg cgctatccaa agggcgcatc ttcgaggaaa cnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntatgtgcaa tttggggcgg
     3181 ctgattttgg ggtggctggt caagatgtcc ttttagaaaa gggtagcgat ggtttgtatg
     3241 taccgatcga tttagggatt gcacgttgcc ggatggcggt agctgttaag aatgggtttg
     3301 attatgcagg ggcggttcgt cagggctcac gcttgcgcgt agcaaccaag tatgtcaatt
     3361 gcgcccgtga gcactttgcg aacaaaggcg ttcatattga tacgatccat ttgtatggct
     3421 cgatggagct agctccctta gttggcttag cagatgcgat tgtcgatcta gtatccacag
     3481 gtaacacttt gcgcgctaat aatttggttg aggtggaatc aatcaccgat atcagtgcac
     3541 gccttattgt gaatcaggcg tcctataagc gtaagcgtac ggccatcaaa aaattactgg
     3601 atgcgtggca gtaatgtcaa aggcattaaa ccaaactgtg atagtgcaac gtttaaagag
     3661 tagcgatcct aattttcaga aaacattatt ggcaagtctt tcattgccat cggccgatga
     3721 tcaagcaatt gatgcagtgg tgattgctat cttggctgag gttaagtcta gaggcgatga
     3781 gg
//
LOCUS       DDPQ01000049            6848 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_25727,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000049 DDPQ01000000
VERSION     DDPQ01000049.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_25727"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1069..1224
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6576..6647
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1685 a         1561 c         1568 g         1804 t
ORIGIN      
        1 atgagcacca ttattgaccc agccatccta ttttttatct ttggtgtatt tgcaggattg
       61 gtgaaatcca atctagagat cccccaacct attacacgct ttctatccct ttacctttta
      121 atggccttgg gcctaaaagg tgggtttgct ctgcaccaat ctgggttgac cttagcaatt
      181 actactagct taggcattgc catcttcttg gcgtgcgttg tgccgctggt gagctacttc
      241 tttctaaaaa ctaaactaag tgcgctggac tctgctgcga ttgcagctac ctatggttca
      301 gtgagtgcgg ttacctttat taccgccacg cagtatttaa atcatgtgca aatcgagtat
      361 ggcggtcata tggcagctgc catggcatta atggaatcgc ctgccattat cttggcaatc
      421 tttttagcca atcgtgccaa gtcttcggta tcacaaacga agcagtctct taaagtcggt
      481 aagttattac atgagtcctt tacggatggt gcgcagcttc ttcttttggg cgccatgctc
      541 attggaattt tatcggggcc tgatggtcaa aaggtgatgg cgccgttctc ggttgatctc
      601 tttaaaggaa tgttggcctt cttcttactt gatatgggct taatgagtgc aaagagtttt
      661 gcagacttaa aagggaagcc gcgcgtactc tttttatatg cattcattgc acccttgtgc
      721 cactccttac tcgcactcac tctttgcctt ctctttcagg ttgagctcgg taatacgatc
      781 ctactcatgg tacttgctgc tagcgcttcc tatattgcgg tacccgctgt actgcgtcat
      841 gccctaccgg aggtcagtcc tgctctgtat atggggatgt cgctcggcat tactttccct
      901 ctcaatatca ttattggcat cccgctctac gccctaatcg ccaaatgggc cttagggtct
      961 tagatacctc agttcccgag cgcgataact gcgcatgagc ctggactcaa agaattaact
     1021 ttggtgatga aggggctagg tatatatatt cgttagaatg aatatatann nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnaagcaa attggtccgc atgtttggct catttactcc
     1261 cctgatggtt ttccaactcc tgagaaccaa gggatgatgg ctaacattac ctttgtagtg
     1321 agtagcaaag gtgtcattct cttggatacg ggtgcctctg tgcaaattgg tgagatggca
     1381 gtgcggatga tcaagcgggt taccaataaa ccagtgattg cgatctttaa ctcccactat
     1441 cacggcgacc atttcttggg caatcaagca tttgttgagg cgtatggtaa agatgtaccc
     1501 atttacgccc acccctattc catcgctcaa atcaagggga tcgaaggtaa tgcctggcgt
     1561 aatctgatgg aacgctggac caaccaagct actgccggta caaaagtgat cgccccaacg
     1621 catgcagcca atcatggcaa tgtatttcat tatggtgatg taagaatcaa gatccatcat
     1681 catggtgtcg ctcacactcc cggcgatatc tctatgcagg ttatcgaaga taagatgacc
     1741 tatgtaggtg atatcgccat gggtaatcgc atcgccaata tcgatgatgg ttcgtatgtt
     1801 ggtactttta aaacctataa aaacctgcaa agttctgagg gtgatcagat atggataccc
     1861 ggtcacggtg agccaagcaa acagctcctc aaggaatacg gtgagttctt ggctggtatt
     1921 tatgaaacct gcgtcaaagc ggttaaagac ggtcaagatc ttagtgttgc taagtcgctc
     1981 gttctaaagg acccgcgggt tagcaaacgg gccaagacca tgcagggctt tgacaacaac
     2041 atcggtaagt acacgagcct tgcctattta gaggctgaaa aagaagcctt ctaagctctt
     2101 tatactgatc ttattagctt tagggctaat accgcccctg ccccgcacag tcaataatcg
     2161 cctgtgttag attttgctaa ttgtgtctgt gaacaccatg cattcaagga ataagttcat
     2221 gagcttgcgt ttattaaaac ctctcttact agtatcgctg tggatgccct tgcttgcaca
     2281 ggcatcgttc atgcccactc atttaattga tggctttgca aatgttatct cgtggatcgt
     2341 tttgattgtc ttaccaattg gctttattgc cttattttgg ttcgtacaca tctggccaga
     2401 taccgtcgct aagcgccgtc aacatccaca acgcgatgcg atccatgcgc tttgtgttct
     2461 ctctttattc tttggcggct tactgtggcc ctttgcctat ctatgggcat atacacgccc
     2521 taccatgtac aagttagctt atggtaccga tcgctactca ccggtagaag aagatcaggt
     2581 gaataaagag tccgattcga gtaaagcgta atggaagtac tcctcctagc catttatgcc
     2641 agcattgttt ggctaatctt ttttaaatac aaattactac cttggacaac tgtctccaag
     2701 gtagttgtgt tcacaatccc cgtagtgggg atgatcacgt tgatcttgct tctcaatatc
     2761 tttgcccctt cctcgggtga cgtcattgtg gttcgcaata acgtgggcat tgtgagtcag
     2821 gtaaagggtc gggtgattga tgtcccggtc aagattaatc agcgagttaa aaagggggat
     2881 atccttttta agattgatgc cacgcaatac caagcccaga cgagctctat caaggctaaa
     2941 ctcgatcttg ctaaattgcg ggtcactgag aacaaacagt tggtcgatgc tggggctggt
     3001 aatcgctttg atctagagaa agcagaagct gatctcatcg atttgcaaga gcagttcaag
     3061 catgcgcaat acgatctcga gcaaaccgtt aatcgcgctc ctgccgatgg cgtgattgtg
     3121 aatgtgcagt tacgcccggg cgcttatgtt gccgccttcc caatttcctc ggtcatgacc
     3181 tttatggaag acaccccaca gatttatgct ctatataaac aaaatgaatt acatcaaatc
     3241 gcgccaggta atgaggctga gttttatatt ccgagcctgc ccggtcagat attgaaggcc
     3301 aaggtcgagt cggtgattta tgccgagggc caaggacaac tcaatgtaag tggtaatctt
     3361 cccaccatcg gtttgcgtga tattactgct aatcgttttg cagttaagtt ggtcttggat
     3421 gacagcaaaa actatccttt attgccggct ggtgcggtgg gtgaaggtgc cgtctataca
     3481 gacagtatgg ccttcttgca tgtgattcgt aaggtgatgt tgcgggttgc aaccaagctg
     3541 aactacatca tccccaaatt gcactaaggt atggcgcacc ctacttgtac tcgctttagg
     3601 attgccgttg gctatacaag tgcatgggtc tgtagcgcat tcctcctgat gagttgttcg
     3661 gtgaatgccc ccataactgg acaagatctt cgcaatcaag ctttaccgca aataagcgtt
     3721 cccaatcaat ttagtacagg cacgaataca ggctctcaac tcgatgatgc ctggctaaaa
     3781 caatttaatg atgctgaact agagactctg gttgctgaag ccttaaagaa ccatcctgat
     3841 gtacgaatca ttgcagctcg gcgtttacag gccgaggcac tgattgatgc agcgggtgga
     3901 gcgcaatacc ctggggtcgg tgtagttggc aacacaggtg gcaaggtcgg ttcgagtggc
     3961 tctggcttaa caggttatta cattggcgct aactgggagt tggatctgtg gggtcgggtt
     4021 cgtacggccg ttgcaggagc caaacagaat gcgcgcgcac tcaatgccga acaggatgca
     4081 gcccgcttat cattggtggg taccttgacc aaaaccgtat ggttagcccg gggcttgcaa
     4141 gatcaggcgc gcttggcaca agaaaacgcc gatgccgcaa aacgttatgc ccaattaaca
     4201 gaaattcggg agcagatcgg ggcttcttct aaaaatgatg tggcctttgc aagaacagca
     4261 gcggcccaag cacaggaagt cgcgctcatc tcaaaacaag caagggatca atccttgcgc
     4321 gcggttgaaa tcctagtggg tcgctacccc caaggggcat tattccaaaa tacacaactt
     4381 ccaaagctac cgccccctat cgccccagga atcccgaccg atattctgga gcgtcgtcct
     4441 gacatcattg cggcagaggc tcgggtcaat agtgcttttt ataccgcttc cgagaaacgc
     4501 ttggcgcgct taccaaagat tagtttgacc gctggctttg gctatatcaa cagtcaggta
     4561 tttagtttgg tcaacggccc cacaagtagt ttgggggttg gcgccaatgt catgatgcct
     4621 ctctttcaag gtggggccat tgaggctcaa atcgcatatc aaaatgctga ggcacaagcg
     4681 gctctcgcta actatggcaa agtagttctt aatgcctttg gtgatgttga gaatgcctta
     4741 actggagaaa tgacttggcg tgagcgcagt gttctcttgg gttcccagtt gaaggaacaa
     4801 aaacaaattc tgcaacggat cgaacaggaa tttaaaattg gtcgtattga tcagcgtcaa
     4861 atccaacagc agaagattaa aaccaatacc actgaaatga gctatcgcca gggacaggtg
     4921 gatgccttga cccagcgcgt taatctatac ctcgctttgg gtggttcccc cactccttaa
     4981 ttctttcact ttctagacca atcatgcact cctcaaacta ccgttacctc atcattctga
     5041 cctctgtgct cctcactgcc tgcagcacgc tggagcgcaa agcggctgtc ccttcgtcag
     5101 atctatccaa agcacaaatt gctggacaag ctagttcgcg ctatctgatt gcaacgagcg
     5161 gggggattaa tacctttgtc gccgacgtcg atgcgatgaa caaaaaaatg aataagcaat
     5221 cgcaagctgg taagagtcat tacttatcac tctcaggtgg tggcgataac ggtgcctttg
     5281 gtgccggctt gcttgttggc tggactgaga acggcacccg tcnncgtccc gcctttgact
     5341 tagtgaccgg aattagtacg ggtgcactaa ttgctccatt tgcctatctt ggcaaagaat
     5401 atgatccggt ccttaaggag gtctataccg agattaaacc ctctgatgta ttcaaagccc
     5461 gcagttttct agcaggtatt tttagtgatg ggcttgccga tacttcaccc ctcttacgct
     5521 taatttcaaa acacgttaat gcggagttct tgcaaaaagt agcacatgag tacaacaaga
     5581 atgggcgctg gctgttaatt gggaccacca atatcgatgc tggcacacca gtgatatgga
     5641 atatgggaca aattgctagc atcggcaccc ctgaatcact cgaacttttt agaaggatct
     5701 tactggcctc tgcttcgata ccaggcgcat ttccacctgt aatgtttgac ttcatgatcg
     5761 atggcaaaga gtttcaggag atgcatgtgg atggtggcgc tacgacccaa gtctttttat
     5821 atccaagtgc tgccgctacg cgcgcaaaag aaattggtat caaacgtcgt gccgagcgac
     5881 aagcttatat tattcgtaat gcgcgtttag acgtggattg gcaagaaacc gagcgtcgca
     5941 cactcagcat tgccggcaga gctatctctc aattaatcca gtcgcaaggc ttaggtgacc
     6001 tttatcgcat ctataacatt acgcaagatg atggggtcgg gttcaatctc gcctatattg
     6061 gtgccgactt taacttcccc caccgtgaag aatttgatac caagtacatg caagctttat
     6121 atgactacgg ctatcaacgg agtaggaagg gatatgagtg gagcaagtat cctcctggct
     6181 atcgtaaatc cattgaggca gataccgagg taaaaccaga acttttaaat acgcctgcga
     6241 agcccacaaa gaaaaaggct aaataagact agcgacaatc aaatactgca aggaggagat
     6301 tatcaccctt gcgataaacc gcttgtttgc catactgttg gcaatacgca ttggccttca
     6361 gtgtcagttg gggtaatgac tcatcggcac tgttcagaaa ggtgacatga ttagcgtccg
     6421 atgcatccgt aaaaacagca gcgcaggctt gcaaaatagg aaacaataca agaacggtta
     6481 agcgagttaa tctcattgga tgcctaatga tgtaaggtct gtagtttaat ctgactagcc
     6541 aattgctcag cctgtttggc gattgcgctt gcagcnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca acagctttag
     6661 caagagcgtc cggtatcgca cagtgatcgg caatcaattg atttaacaat cgatcagagc
     6721 caacgcccgt gcaattgatg atgcgatcgc caagcacctc aactgcagta tttaagacca
     6781 ccctcacctt cgggttcatg gcaatgatct ccttagcacg acccagtaca aattgaattt
     6841 gtccagtc
//
LOCUS       DDPQ01000050            5934 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_17922,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000050 DDPQ01000000
VERSION     DDPQ01000050.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_17922"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    963..994
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3155..3222
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5563..5624
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1478 a         1351 c         1354 g         1589 t
ORIGIN      
        1 ctttgggata gcccacctct gcagtcgttg gtacatcagg tagacttggt aaacgtgttt
       61 taccggttac ggccagcaca cgtaatcgtc ctgttgccac atgttgcata aatggtggcg
      121 ccgttccaaa tgtaatgtca acttgtccgg ccaatagatc ttgtaaagca ggtccagtgc
      181 ctttgtaggg aatatgagcc attttgatac cggcttgttg ttccagcatt gcaccggtca
      241 catgttgcaa ggagccatta cccgatgatg cgtaatttag cttcccagga tttgcttttg
      301 caaacgcaat gaactcttta aaggtcttaa agggtagatc cgcccggatc acaataattt
      361 ggggggctga gatcacgttc gcaactgcct gaaaatcttt ttgctcccat tgctgctgct
      421 ttgacacatg cggggtaatt acgtgatacc cagaatattg catcatgatc atataaccat
      481 ctggaggagc cctctttacc tgtgaagcag ctatattgcc gttacctccg cccttgttct
      541 cgaccacaat ggtttgaccc aatgcagctc ctagtggtgt agccagcatc cgggccgaga
      601 tatccgttgt tcctcctgga gccgtgggga caatcatcgt aatagttctg gctgggtagc
      661 tatccgcttg agaaaatgct tgcccagagc caagaatcag cattgccgat aggatcaaat
      721 gagataagaa tttacggtct tgcatactgg ccccttatat ttatttttag tctcttctta
      781 ataaaccttg cgcttgattg agtgctttat tctggttcag aatttattgt aggctaatat
      841 agatcgatac tattaatttt aaatggggat cctatgactc gctttgtacc gttcatcatt
      901 tctgctgttt gctgtttgtt ctccaccctc gtgttggcct atccaaataa gcctattact
      961 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagcaa atgaagagct ctgtgattgt
     1021 tgaaaatcgt ccgggtgccg gcggtaatat aggtacgcag tttgttgcaa atggtcccaa
     1081 agatggtcat accctattaa tgaccattag tagtgcacag gcgattaatc cagctcttta
     1141 taagaacccc ggttttgatc ctgttaatga tttcgtacca attgcaatgg tgggcgccgt
     1201 tccaaatgta ttggtggtga atgctagttt tcctgcaaaa tccttagatg agttcttaaa
     1261 gctagtgaaa tctaagccac cgggtactta tcaatacgcc tcggcgggta atggcacctt
     1321 aaatcacctc ttaggtgaaa tgctcaatca aagttatggc attagcttgc aacatattcc
     1381 atataaagga gtcgcacccg ctctcaatga tttgttgggt ggtcaaatcc aaattgcatt
     1441 tgctagcttg ccatcggtat tgcaatacat caatactcaa aaattgattg cgcttggggt
     1501 gagttctgca aaacgttctc ctgttctacc cgatgtgcca gctattgctg agaagattcc
     1561 gggattttca ggaaccttgt ggattggttt atttgcacca aaggctaccc caaaagcagt
     1621 ggttgataaa ttacaaaaag agatgcaagc aaccttagct aatcctgagc tgaaagagaa
     1681 attactggca tctggagtag agcttgccaa catcactccg gctgagtttg ccaaacaact
     1741 ccaagacgat attgctcgct gggcaaaaat cgtcaaggca tcgggcgcct cgcttgatta
     1801 gttcctaatt aaacggaatc gtaatcaatc cttctcgagg attcagaggc tgtgcggagt
     1861 cctcccccaa ggctttagcc tctgcctcac cgggctgatc cgtggtgtag ttgggtccaa
     1921 taatgatttt cttgggtgcg ctctcagggg ctggaggctt gggtttaccc atgacgcctt
     1981 gaggtggtac aaggggttgc tcattaatcg ctttaagctc ttcgggctgg aagggagaaa
     2041 tcgattgagc caagaccggc atcgccatga ttgttgtgac aataccaaag gtcattaaat
     2101 aggtcttcat agggagtatt aaaccactct ttatacctct gcctccccta aaatagagaa
     2161 tacatttagc catagatagg ggtttcagat gccagctagt cgtgagcagc tcacagagtt
     2221 ctttgccaaa gactttccac agaccaaatg tcagatcgtt tcagtggaac aagggagtgc
     2281 cgtagtaacc caccctgttg gagtagatga acttcgacct ggtggaaccg tatcggggcc
     2341 caccatgatg tccttggcag atgttgccct ttatgtcgcg atcttggggg aaattgggat
     2401 tgtgccgctg accgtaacca cgagtttaaa tatcaatttt ctgaagcgcc cccaaccgtc
     2461 taatagcatt cgggctgaga gccgtttact aaaattgggc aagaccttag tggttggcga
     2521 ggttaatatc ttttcaggag atgatcccaa gccagttgca catgccactg gcacctactc
     2581 tattccgccc cagaagtaat atcagtcttt acgcttaaac atacccacca ccccagcgaa
     2641 tattttgggt gagagcgata aagcaaatag tagatcaaca ataaagaacc atgagccacg
     2701 cgtcagctca acgatcgcga gtactacaaa gaccgccatc atcactcggc gtaatgtatc
     2761 ttgtggaaaa taacccattg cgtaatcctc tttggtttat tgctgcagtt tagatgattt
     2821 gtacgacatc atcaaaagcc aatcgtggtc cacgtgcata aatacctgca ggatcagcga
     2881 caccaatatt gagtaagaag tttgctttat agcgaccatc cgtaaagaac tctgcattga
     2941 ctttggcagg atcaaatccc gacatcgctc cacaatcaaa gcccagactg cgaaccgcca
     3001 tgattaagaa cgcaccctgc aaagagctat tgcgcagacc agcaacctcg gtcaccgctg
     3061 gcttaccctc aaaaaatggc ttagcatcca ctgctggata taagacaggc aagtgctcat
     3121 aaaagagagt atcaaaggct acgatgaccg tgagnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtcctgg ttgggcatta
     3241 aatgcggttg gagcccactt gaataactca tagagctcct gaatctgttc atcactgatg
     3301 tcgtgctgtt taaaagcatg cactgtgcgt gcttcggtaa atagttcttt ctggatatca
     3361 atggataaag gttttttcat ggattggctc aacagaatta aaatagaaac attcttccat
     3421 tatcaatctt tttactatga gctcccccaa tcctattact gcaactctga tccctggcga
     3481 tggcattggt cccgagaccg ttgagtccac catccaaatc ctagatgccc tcggcagccc
     3541 ttttctgtgg gatgtgcagc aggcgggggt ggccggtgtt gatgccctag gtgacccatt
     3601 accagaacaa accttggcga gtatccgtaa acatcgctta gccctcaagg gtcctttgac
     3661 cacgcctgtg ggtggaggct ttcggtcttc caacgtgcgc ttgcgtgaag agtttgagct
     3721 ctatgccaat gtgcgccctg ccaagacact gataccgggt cgctttgaga acatagatat
     3781 tgttttggta cgtgagaact taggtggctt ctatgttgcc catgagtact acatccctgt
     3841 gggtaatgat cctaaggcgg ttgctgtgtc gaccggaatg aacacgcgtg agagtagcaa
     3901 acgtatcgcg cgctttgcct ttgaatatgc tttgaagaat ggccgtaaga aaattaccgt
     3961 cgttcataaa gccaatatcc tcaagttact cactggcatc tttttggatg cggctcgtga
     4021 ggtcgccaaa gaatacgagg gccgagttga gatggatgac atgattgtgg atgcctgtgc
     4081 catgcaattg gtactcaatc catggcgctt tgatatgttg ctctgtacta atttgtttgg
     4141 tgacattctg tctgatcaaa ttgcaggcct cgtgggcggt ttgggtatgg cgccgggtgc
     4201 caatatcggt gagagcgccg ccatctttga ggccgtgcat ggctctgctc ccgatattgc
     4261 tggcaaaggg gttgctaacc caatctcgat cttactggcc tctgccatga tgctcgatca
     4321 tgtaggtaaa caagatcttg caacccgtct acgcaaagca attacagata ccttaaatat
     4381 cgactcgata cgcacgcgtg acctcaaggg ggaggcaacg actaagcagt tcacagatgc
     4441 ggtgtgcaag cgtttagcga gtggctaata aggtaaacca ctagcgatat ctgcaggatt
     4501 tagtgataaa aagagagcat gacattgact aagggattaa tgatgcgtca tattattact
     4561 tcgttcactt taattctgat tagctttata agctttcatg tacatgctca aagttatccc
     4621 aatcgcgcta ttaagttagt tgtacccttt cctccgggcg gcttaattga taatgctgcg
     4681 cgactcattg gtccagagct ctccaaagaa ctgggtcagc ccgtggtgat tgagaacaaa
     4741 ccaggagcgg gtggcaattt agcagcagcc gacgttgcaa aagcgagtcc agatggctat
     4801 acccttttaa tggcctccgc tccactaagc attagccctg ctctgtataa aaccttgcct
     4861 tacaacccag tcagcattga acccattgct gttctaggac agcttcccaa tgtcttattg
     4921 gtcaacccag aatcggggat taacactgtt gccgatttag tcaaacgagc gaaggcaaat
     4981 cccggaaaac tcaattacgc ctccaatggc aatggcacat cactgcatct cagtgccgag
     5041 atgctcaaga accaagcggg tatctttgtg gttcatatac cctatcgtgg ctcggcgttt
     5101 gcaaatgttg ggctggtatc aaaagaagtg gattttatgt ttgataacct accgcctgct
     5161 cttggcctaa ttaatgggaa caaattaaag cctctagcag ttactactgc gacacgcaat
     5221 cccgcccttc ccaatgtgcc gaccatgatt gaatcaggct atcccaactt cgatgtttct
     5281 gcttggtttg gcttagctgc cccaaaagga atacctgatg gcgttagtca gcgtttgcag
     5341 gttgctttag aaaagattgt ggctcgcaag gaaatggttg aggcaatctc acgtttaggt
     5401 gctacggtta catacatgaa tgcgcaacgt gccgctcaat ttatgaagac cgatactgat
     5461 cgctggcgta aggtgattga ctatgccaag atccagatgg attaggcaaa accttaatat
     5521 ataaaggggg tcaagtgacc ccctttattc tttgatgaga acnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctggg tcacgatgca
     5641 ccactacaga acgaccaaca atacctgctg gacctgtgtt gactgtgaac ccagttaatt
     5701 tggcagagta tgtagcatta ccattggcat ccgctttgat attgggcatg tcaccagcat
     5761 ggctgtgtga actacctggc tgtccgtgtg ccttaccatc tggattgaaa tgacctgccg
     5821 cactcattgc atcgggtgca gagcaatcgc ctttttcatg aacatgaaat ccgtgctctg
     5881 aatttggctt aaggccactt accttagcgc ttaccaagac atcattgccc tgcc
//
LOCUS       DDPQ01000051            5397 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_46342,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000051 DDPQ01000000
VERSION     DDPQ01000051.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_46342"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1081..1186
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1979..2050
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4418..4513
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1235 a         1271 c         1298 g         1317 t
ORIGIN      
        1 attgataact tcatgatcca aggcggtggc atggccccag gtatgaaaca aaagcccacc
       61 aatgcggaga ttgagaacga agccaataac ggcttaaaga acgtcaaggg ttcgattgcg
      121 atggcacgaa ccaatgagcc gcattctgcc agctctcagt tctttattaa tgttaatgac
      181 aacgatttct tgaaccannt ggggctatgc agtttttggt tcggttagcc atggcatgga
      241 cgttgtggag aagatcaaaa aagtgcctac cggaaattct ggctaccatc aagatgttcc
      301 aactgaggac gttgtcatcg agacggcttc tgtaatcgaa gactagttca tgcaagaaca
      361 cgctagcgcc ctgctgatct cagatctgca tttgaccccg tcaatgccct tgacggcgca
      421 gcgtttcttt gactttgtcg agaaggaagc caaccaggta gagtctgtat ttatcttggg
      481 tgatctgttt gagtattggg tgggagatga tgctggagca cgctcccctt ttcagcaaga
      541 ggtcttgcgc gccttaagtc gtttatctag caaggccaaa acgtattaca tccatggtaa
      601 ccgtgacttc ttaattggtg agcagttcat tcgcaaatcg ggcatgacct tgttaccaga
      661 cccttctaag gtaatgatcg cgggtgatga atgggtcatt agccatggtg atgccttatg
      721 caccaatgat ctgagttatc agatattccg taaatgggtt cgtaagcctt ggttacaaaa
      781 tctgttcttg cgcctacccc ttgagtggcg acgcagtatc gcacgtaccc tgcgaactaa
      841 tagtacggct aagtatgatc gtgcggttcg ttatacccca gagatggctc ggttcaaggg
      901 tgatgtcact ttggtggcct gtgccaatct catgggctca tatagctcag agcggttgat
      961 ccatgggcat acgcacctac ctgcccgaca tcatgaaagc atgggcaata aagagtggca
     1021 acgttgggta ctatctgact gggatctaga tcatcctgag accgtgttgc ctaaagcgag
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaga tacgcggatt
     1201 gcccgccata atctctccgc tctctaagaa tccttcttcg ccccgatagt taccaaccaa
     1261 accacctgcc tcggtaatga tgagtgcgcc ggcagccata tcccaaggct ttaactcgct
     1321 ttcaaagaag ccgtcatacc ggcctgcagc aacataggct agatctaatg aggcagcgcc
     1381 cgggcggcgt aagcctgcgc attgacgcgt catgtccgca aagatcttta agtattggtc
     1441 cacatcttga tcttggcgat acggaaatcc tgttccgatt aaagcgttct ctaatttaat
     1501 ctgatcagct actcgtaaac gacgacgatc aacgtaggcg ccaccgcctt tgctggcaga
     1561 aaataactca tcacgattag gatcataaat gaccgcttgc tgcagtactc cgtttaccga
     1621 caaggcaatg gagatcgcat attgaggaaa gccgtggatg aagttggttg tgccatctag
     1681 aggatcaatg atccaaatat gatcggagtt atttaacagc tcaccagact cttcggccaa
     1741 gaacccatga ttggggtagg cctcactgag ggtctcgatg atggcctctt cggccgcttt
     1801 gtccacttcg gtcacaaaat cgttatgttg cttacgatcg atttgcaagc gctccaaatt
     1861 aagtgaagcg cgattaataa tcgttgcggc acggcgtgcg gccttcacgg ccacgtttaa
     1921 catggggtgc ataatcagtg gacaaattaa ataagaacaa gctccagaga cataaaagnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn aaaactcatt ttctgtgcat tgcgatcagg tccgttgggt cttggtcgaa
     2101 actagtcatg cgggcaatgt gggctcgacc gcccgcgccc tcaaaaccat gggctttgag
     2161 cacttacacc tagttcgtcc acggcaagac agaatgcatg aggatgctga agcaatcgcc
     2221 ttagccagcg gtgctagcga tgtattggct aagactactg ttacccaatc cctctctcaa
     2281 tcactccaga attgcagtgt tgttctcggt ttaagcagtc gtgaccgcga gtttggtcct
     2341 cccgccttag actggaatag tggtttagat cttttgctcg atgctgtgag taaccagcaa
     2401 tcggttgcaa tggtctttgg tccagagcgc actggtctag agaacgaaga tctggctttt
     2461 tgtacacacc gggtttattt gcctgctaac cctgactacc cctccctcaa tctggcgcag
     2521 gctgtaatgg tctgtgcctt tggcttacgc caagcccttg tgcaagggca tgagcacatc
     2581 ccccagaccg atcctgccga gcttgccgat ccgattgcgg tagcagcaat gctagaacat
     2641 tggcaagcag ggcttgaggc catcgggtat ttggatccta atcatcccaa gaaattaatg
     2701 ccgcgcctgc agtctttatt tgcaagagcg cgtttacgca aagaggagat tgatctcctg
     2761 cgggggattg cgaaacagat gatcctgagg aaatagccac tctcccatta cactaatggg
     2821 atgctgagct cattactcga agacgtcgat gccattattg cgcgggatcc tgccgcccgg
     2881 catcgtctcg aggtaatcac ctgctaccca ggccttcatg ccatttggct gcatcgcctc
     2941 aatcacgcac tctggaactt aggtcttaaa tggcccgctc ggttcttggc gtccatagct
     3001 cgttttttga ccaatattga gatccatcct ggagcgcgtc ttgggcgtcg ggtgttctta
     3061 gaccatggtc ttggcatcgt cattggcgag accgccgaga ttggagatga cagcaccatc
     3121 tatcaagggg tcactctagg tggcacctcg ctctataaag gggtgaagcg tcaccctact
     3181 ttgggcaaag gggtcgtggt gagcgctggg gctaaggtct taggaggatt tacggtgggc
     3241 gatggcgctc ggattggatc gaatgcggtg gttttaaaag agattccagc aggagccaca
     3301 gcggttggtg tgccagcacg tatcttgcat cccgatcttc ccaaagatgc aagcgatgcc
     3361 agtaaggcca gtttctcggc ctatggcatt acgcctaatg ctgatgatcc agtatcactg
     3421 gcgcttaaga gtttgatcga tgcaacgatc gagcaagaga acaagatcaa ggcacttgag
     3481 gaagcgattg ctaaattggg tgggaaagcc agcgatgatc atggccatca agagaccaag
     3541 cgagagcttg acgcaatcaa gcaacttctt aaagagtagt ttgctttaat gtaaggtgcg
     3601 ggggctagta ccagatcctt tggcctcgcc accattccga tctagctcat ccagatcgtc
     3661 ttcatcggcg agatcaagac caaagccatc ttcatcctgt gggtgctgat tcgcaatctc
     3721 gtcatcggtt gcttggcgaa catcttcaac ctgtaaccaa aaacgcagag ccattccagc
     3781 caagggatga ttaccatcta agaccacttg tttatcggcg agctcggtca ccgtataaat
     3841 taaggtatca atctcttcat cgtcctcatc ttcgtcttca tcggcctcta gaatctcatc
     3901 ctctgataat tcttcgggca ctccttcaaa ctgcatcccg atctcgagag gttcggggaa
     3961 gcgctcgcgc ggttctattt tgagtagatt ggggtcgtac tccccaaacg catcatgggg
     4021 ctctagttgc acagtggtct cgaaaccaac atccttccca tcgagtaagc tctcgatctt
     4081 tgggaaggtg cccgagtacc cgccatgaag gtataccatg acggtatcgg gttcttcgat
     4141 aatgttgttt tgtgcatcgc tgagtttgta acggagagaa acgatggtat ttttttcaat
     4201 cttcatagcg atgtagttcc tgaataaggc tattttactt gactaagacc gattcaatct
     4261 atcaggcgcc tcgagcccca gcccagcttc ccctcgatga gccctgggcg cttttgggcc
     4321 agcgaagccc taatgccttt atgcgggact attggcataa gcgccccttg ctggtccgcc
     4381 atgcaatccc cagttttgcg cttgcaaaag ctcagggnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnagtccaa tgactggact ttattggtcc aaggggtcga ggcacggcac
     4561 cctgcggctg ctaatgtctt atcgtggttt aggtttattc cggatgctcg tcttgatgat
     4621 ctgatgatta gcattgctgg tgttggtggt ggcgttggtc cccatgtcga ctcctacgat
     4681 gtctttttga tccagatgga gggtcggcgc cgttggaaga tatccgcaca atccgattta
     4741 tccctccggg acgatctacc cctcaaaata ttgtcgcgct ttgaggcaaa agaagattgg
     4801 gttctggagc ctggagatct tctatatctt cctcctcaca ttgcgcatga aggtattgcc
     4861 ttagatgcgg gctgtcagac ctggtcagtg ggctttcgat cgccaagcta tcgcgagctt
     4921 ttgcaagagg gtttatggcg tctcgcagaa tcccttgaag atgaaaccga actcgccacg
     4981 atttatgcag atcctcttca aggcgccacc aaggatccgg cagttttacc aagactccta
     5041 gaagagcaga ttagcaaaca cgttcgtaaa ctggcattgg gtgaaggtaa gaaatatctt
     5101 caaggaatca gcgcctatct gagcgagcct aagcctcagg ccgtttttga gggtccgcat
     5161 gatgcaatga tgccaaaggc attttggcaa acactctatt ccaagaccct tgttccccat
     5221 ccgcagactc gcctcctaat ttggggggat gaagtctttt gcaatggcga ggcggtatcc
     5281 cgtgggcaaa ctccagcagt tcaaaaagcc tggaaagaac ttgctagatc acatacccta
     5341 gccctagggc gtagggctaa aaaatcaagc aaaaataagg tcttagtgga tacaaat
//
LOCUS       DDPQ01000052           20924 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_10380,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000052 DDPQ01000000
VERSION     DDPQ01000052.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 20924)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20924)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20924
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_10380"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    246..317
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    880..1011
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1193..1237
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2212..2355
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6207..6256
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7375..7419
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13352..13364
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17839..17894
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5663 a         4540 c         4799 g         5353 t
ORIGIN      
        1 agtaatgcaa actctcttga tgctactccg caaaatctaa tcgttaccca aactctagca
       61 cggcatatat tgattcggca acgccaaggt ctgcaggatg ctgatgttga gcgacgcctt
      121 cagggatacc gtgatcagat tcgcgcgaag accgcagatt ttgagtcgat ggccaagaaa
      181 ttttcagagg atggctcggc tcaaaatggc ggcgttttag gctggatgag tccgggcgat
      241 cttgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnngat ccagtaaaga ctgaatttgg atggcattta attcaggtaa
      361 tggagcgacg tgaagcgcag ctgacggttg agaagcaacg ggagtttgcg cgtgcagcga
      421 tccgagaacg taaatttgag caggcttatc aagactggtt gcgacagtta cgcgatacgg
      481 caacagtcaa aattttgaac gtggatgaac agtaccgtta agttagcaat tagtactggc
      541 gagcctgccg gtatagggcc agaggtatca attgcggctg caaacgcatt tattgcagat
      601 aatcctcata ccaaaattac tctctatggt gatcgaagtc tgctgcaatc tcaaagctta
      661 gtaagccaaa ttcagattgc gcacatcccg ctcatcaagc caaaccaaat tggtaggctt
      721 aatccagaaa atgcccccta tgttctttct gtaattcaag agtccagtga agcctgccgt
      781 tcaggagtgc atgacgcttt gataacagcc cccgttcaga aaagtatatt aagcagaccc
      841 tcgttagaat ttagtgggca taccgagttc ttggcccaan nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagtggccc
     1021 aatcgattca atacaagtct ttattgcaaa ccattcgtat tattcattct gatctacaaa
     1081 cccgctttgg tattgctagc cccatcatta tggtgactgg tttaaatccc catgctggag
     1141 aggatggaca tttagggcga gaagaaattg acacgattat cccagcgatc aannnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt tttaatccag ctcgtttaca
     1261 ggctgctgac gccgtattgg ccatgtatca cgatcaaggc ttagccccct tcaagtttgc
     1321 aacgtttacc gagggtgtga acgtgacttt gggcctctct tatattcgca cctcagtgga
     1381 tcatggcact gcactggata ttgctggaca cgataaggca gattggcaca gtatgtacgc
     1441 cgcactcaac ttagcccaag agctcacctt aagacagaag cgttttaatg catcaggcac
     1501 gtaagcgatt tgggcagaac tttctaaccg atggcagcat tgtttcgggg attgtgcgtg
     1561 ccctagctcc cgaatcaaac atccatctct tagaaattgg tcctggtttg ggagccttaa
     1621 cattaccact gctctctcag gtagaccatc tggatattct tgaaatcgat cgtgacctgg
     1681 taagttattg gcaggagcaa aagcagaagc aagctttaat tggattaaat atcatcgagg
     1741 gcgatgcgct tgaatttgac tttctatcat gggctcagcg gggtaaagag gcgaagcaac
     1801 gcagttgcat catcggtaat ctgccataca acatctcttc accactactt tttcatttga
     1861 tctcagcagc accctggatc gatgagcagg tttttatgtt gcaagccgag gtggttgaac
     1921 gaattacagc ggcagcaggt gattctgaat acagtcgttt atcagtcatg cttcaagcgc
     1981 gctactatgt agaacaaatc atgaatgtac caccggaggc ctttgagcct aaacctaagg
     2041 tgaactcagc agtagtccgc atgatcccgc gccgtgattt tgatttgacg gcgaaggaat
     2101 gggacgcctt aagcaagatc gtagcgatgg catttgcaca acgacgtaaa atgctgcgta
     2161 gtaacttaca tgccttccaa gcagagttaa acctttctga tgaagagctg annnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnccagg gtacttcaag aaactattac ggggccagaa atgaccggca
     2401 ttatgagcaa caggaagcac cgtcgcctgg gtcaagcctg caagtcgagc gccacccttg
     2461 cgatagggct tatgggagcc aacaggtgtg cgcgttcctt ctggaaacaa aataatccat
     2521 ttgcctgctt gtaaacgtgc ctttccttga ttggtgaccg agattgcgga actctcttta
     2581 gccgagcgat tgatatgaat catattgagt aatcccatga cccagccaaa gaacggaatc
     2641 cataggagct cacgtttaaa cacatagcaa agatcttttg ggaaaagatt taaataggca
     2701 atggtttccc aagcagattg atgcttgcta aggatcacca ccgcttgatc acgtactgca
     2761 ttggcatgct ccataccaat tacctgataa cgaactccac aaaagaactc cagaaagaaa
     2821 ataatacttt tcccccagaa ctgaatcagc tgatagcgct gatgttcgtt gagaaagggg
     2881 aagctcacta agcaaaaaac ggcatagggc ggcgtatata gaacgaggct taaaaagaac
     2941 aagagggatc gtagccagat catgatgttg ataggatgta ttttgtaaaa gccattagat
     3001 cagcatgagt cttggtgcct tcgggtaact cagcatcttt gaccttatgg cccttgccag
     3061 ttaggacaag atggggcgat gctcctaatt tagtacctgc aacaagatcg cgaagagagt
     3121 cgccaacaat cggaatattn nnnnnnnctt tggtttgcgg cactcgcaaa gatcctcatc
     3181 aacatggggg caaaagaaaa tcccatcaat cctgccgcca aggggcttga gtaaatcgat
     3241 cattttttga tgcatagcat gtaactcaga aagcgagtaa aggcctcgag caatccctga
     3301 ttgattagta gcaatcgtta aaacatatcc tgcctgtgag agcaaagcaa tcgcctctaa
     3361 gctattgggg atcggtatcc actcatctct agatttaacg tagtcatcgc gatcttcatt
     3421 gattacccca tcgcgatcta aaataatgag cttacttgtc tttggactca tgcaagtttg
     3481 ccgatatcgg ctactagatt cattttctga tgtaactggc ttaaaagggc aatacggttt
     3541 tgccgaagtt gcagatcatc atccatcacc attacatcgg caaaaaattg atcaataggt
     3601 tgactaagga ctgcaagact ctgcagtaaa gtcataaatt cttgattctt aaactgctga
     3661 tctagttttg ggcttagagt ctctaaagcc ttataaagat tcgcctctgc gggaagacgc
     3721 agtagctgaa cttgcacatt ttgagccgat tgaccctcaa tctttttcag aatattacta
     3781 atacgcttat tggccgctgc taattgggcg gcttcactta gttgtgtaaa tccacgaata
     3841 gcctgtaaac gcttgggcag atcatttagc ttacccgggt tcatgctcaa gatcgactca
     3901 atttctgggg ctgtaaagtg agcatccccg agtaattgat ccttgaggta agtttttaat
     3961 cgctctagaa aaaattgata gatggcttcg tcatcaattt cattcttaag tgaaagaccg
     4021 acaaattgtt ttttggccat tgcgattaat actttgagat ctaaggccaa attcttttcg
     4081 ataagaagcc ggcagattcc tagagcatgg cgacgaaggg caaatggatc cttatcacct
     4141 gttggtacta agcctactcc ccaaatcccg acaatggtct ctaatttatc ggcaatggca
     4201 agagtggtgc caaccatggt ttgtggaaga gcatcaccgg caaaacgtgg caagtaatgt
     4261 tcatagcaag ctgcagcaac atccggatgt tctagatctg ccaaagcgta gtattgcccc
     4321 atcacccctt gaagctctgg aaattcgcca accatatcac taagtaaatc ggttttggca
     4381 acttcggcag cccgaccaac tattttggga tcgatagcaa tcccagtttc gtccacttgt
     4441 ttagcaatct caccagccag agctcgtaca cgctccatcc gctgtaattg attacctagt
     4501 tgattgtgat aaaccacctt agccagatcg gggatgcgag actctaaaga acgcttctga
     4561 tcttgttgaa agaaaaagcg cgcatcggat agtcggggtc taataacgcg ctcatttccg
     4621 gagataattc cttcaggtcg atcggttacg atattagaca caatcaaaaa gcggttcttc
     4681 aacttaccag taccgtctgt caaagcaaaa tacttttgat tggtttgcat ggtcaggatc
     4741 aggcactctt gcggaacatt taaaaattca ggatcaaagt gacaggtata gatcgcagga
     4801 tattcaacca gggcagtgac ctcatccaga agagaatctg gcatgagtac ttggtcagac
     4861 ttcgctgcgt ttattaagtc agtctcaatg agttggcgtc gtttagtaaa tgaagcaatt
     4921 accttacctt cgcgctcaag tgcttgttca taatcgacgg cagactgaat ggcaattggg
     4981 ccagaagaca taaagcgatg accctcagta gtgttgcttg ctgtaatacc gaaggcttga
     5041 atgggaagca catgagagcc atgcatcgca acaatacgat gaacgggtct tgcaaactca
     5101 acatcctgaa cactgccatt tgcatcgttt acttggtaat gcatcatttt ggcaatcggt
     5161 aattgcttga ttgcttcttc aatagccttt tgaagactac tttgtagatc tgcccctttg
     5221 gcagtaacgt tgaggtaaag agcctcattc ttaccctcac cacttttttc taactgctca
     5281 atagtggtgt tggcataacc caggctcgcc agtttctttt gtaggggcgc acttgcttga
     5341 ccattttcgt caaatgcaat tgatgtgggc aagagtttct cacgcacagg atagtccgga
     5401 gcttgctcta gaactgcact gattaatact gcgagtcgtc gtgggctagc aaaggattgg
     5461 ctggtggctg attgattgac taagtgatcc tggcttaggc attgaaaaat agattgtgca
     5521 aaggagtccc ctaagcgctt gagcgacttg ggaggtaact cttcagtaaa gagctcgact
     5581 aataaggcgt catgtttcat ggtgtgagtc atgccttacg tagatttctt ctggagcata
     5641 ggaaagccaa gctgttcacg agactcaaaa taggcttgag caaccgaccg tgacaaatta
     5701 cgaatacgtc cgatataggc ggcgcgctcg gtcaccgata tggcgccacg tgcatcgagt
     5761 aagttaaagg tatgtgctgc tttaagcacc atctcataag cagggagcgc taatgaaagc
     5821 tccattaagc gttttgcttc gctttcataa ttactgaagt tattaaagag catctcagtg
     5881 ttggagtgtt caaagttata gcgtgattgc tcgacttcat tttgatgata gacatcgcca
     5941 taggacacgc catcggtcca cactaaatca aaaacgtttg aacagttttg taaatacatc
     6001 gccaagcgct caattccata ggtgatctcg ccgagaacag gcttgcaatc cagaccacct
     6061 acttgctgaa agtaggtaaa ttgggttact tccataccat ttagccaaac ttcccagcca
     6121 agaccccagg cgcctaaagt gggattttcc caatcgtctt cgacaaagcg gatatcgttt
     6181 tggtttagat ccaaacccaa tgctctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnncgat tggggttctc accatagcgt ccgtctttag gacggcgtga
     6301 tggttgaaca taagcagctt tccatggctc aggaccaatg gcgcgcaaga aggtggctgt
     6361 atgggaggtg ccagcaccaa cctctaggtc aatcggttgc aagagcgcgc agccctgctg
     6421 atcccagtag tcttgaagct taagaatgat ttgctgaaac gttagcatgc caagtcgacg
     6481 agtttagtaa ttacgatatt aagaccttga ttttactcgg caaagaatgg tcgggctcaa
     6541 aagaaccctt tcttactttc gaaatcgtag gtaagcgctt agcaagagaa aaagagaggc
     6601 taataagatt ggccaattgc cccaaatcac ataaggggtc tgccccgtat agggctgcac
     6661 caacccagag agctcccctt gtacaaactg atctaaggcg gtctgtacct caccgcgatg
     6721 atcaatgatg gctgtaatcc cggtattggt cgctcgtaaa ctgggtaagc ctgtttcgat
     6781 ggagcgtaat tgtgaaaggc gtaattgttg catcggcgcc tgcgattgcc caaaccaggc
     6841 caagttagtg acattgatta aaagattgac cgggttttgg ctctggcgta ggcgactggc
     6901 gatctcattg ccaaaaatat cctcatagca aatagagata gcagcatagc gtgctttact
     6961 gggatcctga tcgcgtccta gaacagtaaa tggagcctgc gagaatgaac ctcttgcaaa
     7021 attactcatg ggcactttga aggcatcgac aaaccattga aaacccggcg ggataaattc
     7081 tccaaaggga acgagatgat atttatcgta gtggtatcgg ctttgatctt tcgtaaagcc
     7141 gctgactcga ttggagtact ggaaactttt ttcatctatc gataccttac caatcagacc
     7201 taataaaact tgactacctg attgattggc aaagctttga atttgctcaa gagattgatc
     7261 gggtaaatcg tgttcaggcc acgggaaagc ggtttcagga acaataatca aatcagcagg
     7321 cgctttcata atggtatctc gataaaaatc gatctgccgc aaaatttcct tgggnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ttcccaatat ctgtgtgata
     7441 gcgatcacac ttagcacagc aagcgcatta ccgatcttta atagactaat ttgccaagat
     7501 gcccaaacaa ctaagaaggt acatgcgagt ccacccaggt aaggcgcaat ttgtataaag
     7561 gggccatgca cttgtgactc tgcaagaccc atccaaggaa atcccgtgaa caactgaccg
     7621 cgtaagtatt ccgccatcac ccaggcggat ggtaaggcaa gaccacgcca gaggggattg
     7681 gtaatgagca gtccacaacc aaaggcgagg ccaacataaa tcgccatcag acctgctaga
     7741 gcaaagacgg caaaagagga tagcagcgta gatatccctc ctacatcatg gagactgatg
     7801 aagatccacc acagggcaac gatgaaataa cctagaccaa aaatcaagcc ctgattaaaa
     7861 aggcggacca gtgtttgctc ggcccctttg gcacagacgc gccacacaaa ggccaataaa
     7921 aggatgtgga tgtatccact taatggtaat tcactcgaga ttgctagtag tactccagca
     7981 gcgaacagta aagcaagatg aatcagagga agcgatctga cggtgcttac cgacaattag
     8041 gcctcggtcg gactatttgg attaggcaca atacgacgca tgagaagaat atgaacctgt
     8101 cttgggtcag cgcgttgaac ctcaaactca aattcattta ctcgaacccg ctccccaaat
     8161 ttgggaaccc gcccaaggtg ttgtatcact aaaccagcaa cagtctcgac atcgtccgcc
     8221 tcaaattgag tatttaaatc ctcatttaat tgctctagct ctgttattcc tttaattcga
     8281 taatcaccat tttttagaac agtaatgttg tcggcatcct catcaatgtc atgctcatct
     8341 tcgatatcac ccacaatttg ttcaatcacg tcttcaatcg tgatcatgcc agcaacgcca
     8401 ctatattcat caaccacaat ggccagatga ttgcgattat tacgaaagtc ttttaataag
     8461 atgctcaaac gctttgactc cggaataaag acagcaggac gcaaccaatc acggatttga
     8521 aaatccttct cggtaaagtg acgcaatagg tctttggcca acagaatgcc aattacttga
     8581 tcacggctac cctcaaagac cggaaaacgt gaatgccctg ttttaacaac atccgcaatg
     8641 atctgcggta ggggagagct gacatcgatc caatctattt gggctcttgg taagagaata
     8701 tcccgcgccg atagctcgcc cacctgaaag accccttcga tcatcgagag ggcgtcagca
     8761 tccatcaaac catcaatctg tgcctctcgc agagtttcta ttaactcctt gcgttgagcc
     8821 ttgggatcag agggttgaac agagaaaaac gcgcttaagc gatcaatcaa gctatttgac
     8881 atagattaga catagcataa aacattttga tgacaagatc ctattgaaga aaactgttat
     8941 caatagggat tggcaaagcc gatagaagcc agtaacttga cttcaagggc ctccattttc
     9001 tttgtttggg ccgcattcat atggtcatga ccctgggcat gcagacagcc atgaatgagt
     9061 aaatgcgcta aatgggcctt caataactta ccttgctctt ttgcttcttg gcgcacaatc
     9121 ggcatacaca aaacgatatc ggcatcaatt gcttcaccct tgttgtgata tggaaaagta
     9181 aggacattgg ttggtttatc aaagccccga aagaacagat ttagatcgtg cgcctctgct
     9241 ttattaataa accgaatcgt aatacgagca ggttttgtaa aacactttct ggaccattgg
     9301 ctaagctctg tctcggaggc gatcgacatt atttgggttt gtagtgcaga acttgcatat
     9361 tgcagttcga tggatggtaa tttaggcatg gatcttttct tcaggcgggc acataagcaa
     9421 gttcgccacg tagggtatgt ttaagaactt cggtgacctt gacatctaac atctctccga
     9481 tccagctatc tgcttggggg tgagcgacat cattgggggg aatgaggata ttaatcacgc
     9541 ggttgttttc agtgcgacct aagagatggg cgccatcgcg cgtgaatccc tcaatcaaaa
     9601 cacgctctgt tttatctagc atggccttgc taaccacaag tgcttgcgca tcaatttgag
     9661 caatgaggcg ctgtaagcgt ttgagtttga tcgcgtaggg agtgtcatcc attaaattgg
     9721 ctgcaggggt tcctgggcgg ggactaaata taaaggagta gctattatca aaattaagct
     9781 ccaccaccat ccccattaat ttttcaaagt caacatcggt ctcaccagga aagcccacga
     9841 taaagtcgct ggataaggac agattaggac gtacagcacg catcttacga atgatgctct
     9901 tgaactccag tgcggtatag ccacgcttca tcgcagcaag aatggtgtca gaggcatgtt
     9961 gaacggggag gtgtaaatgg ctaaccagtt gaggcacttt gtcatagaca tcgattaatc
    10021 gctgtgtgaa ctctttgggg tggctggtcg taaaccgaat ccgatcaagc cctgggattt
    10081 cagacatgta ctcgagaagc agggcaaagt cagcaatctc cttgctattg cccatcgtcc
    10141 cgcgatacgc attgacgttt tgtccaagca gggtaatttc ttttacgcct tgttcggtaa
    10201 gaccaactat ttcagtgaga acatcttcaa agggccgtga cacttcctcg ccacgcgtat
    10261 agggaaccac gcaatagcta cagtatttgg agcaaccctc catgatggat acaaaggcag
    10321 agccccgtgt ttgacgcgaa cgagggaggt gatcgaattt ctcaatctct ggaaacgaaa
    10381 tatccacttg ggatttacca gtttctagtc gtttagcaat taaatcaggt aggcgatgta
    10441 aggtttgtgg gccaaagaca aagtcaacat ggggcgcacg gttcacaatt tgcttgccct
    10501 cttggctagc aacacaacca ccaaccccaa tgagcaaatc aggcttattt tgtttgagct
    10561 cacgcagacg ccctaaatca gaaaagactt tttcttgggc tttctcgcga atagaacagg
    10621 tgttgagcaa gatgacatct gcttcttcga tgtcactagt attactcatg ccttcggatg
    10681 aatggagtac atcggccatt ttttccgaat cgtactcatt catttgacaa ccaaatgttt
    10741 tgatataaag ctttttcatg tgccgttctc aggtttatag ctgggggcta agctagattg
    10801 tagtcgaagc gcccatatgg agccctcgtc aaggggcttg aaattgggca atcagtcccc
    10861 atataaggca tatccattaa tactgaggta agccatgact atgcaaaaag aaaccctagg
    10921 atttcaggca gaagtaaagc agcttttaca gctgatgatc cactcccttt attccaataa
    10981 agaaattttc ttacgtgagt taatttcaaa tgcttccgat gcggcagata agctccgatt
    11041 cgaggctatt aaccatgccg attggtacga atctgatcct gagctcaaaa ttcagatcag
    11101 ctttaataaa gaagcgagaa ccgtcaccat tgccgataac ggaattggga tgagccgcga
    11161 tgaagtgatt gccaacttag gaacgattgc ccgctctgga acgaaagaat tcttttctaa
    11221 gctttcgggt gacgatcaaa aagatgctgc attaattggg cagtttggag tgggtttcta
    11281 ttcggcattt attgttgctg acaatatcgt ggttcagacc cgtcgcgcag gcttacctgc
    11341 tagcgaagga gtgcgctggg aatctcaagg ggctggcgag ttcacgattg aaaccatcaa
    11401 tcgacctgag cggggaacgt cgattaccct acatttgcga gaggaagaag atgatctcct
    11461 ctcaagctgg aaactcaaat ccattattcg gaaatactcg gatcatattt ccctgcccat
    11521 tcagatgcaa aaagaagagt gggatgaggg caaaaaagag caggtacaaa aggatgagtt
    11581 tgagaacatt aatcaagcga ctgcattgtg ggcgagacca aaatcggagt taacggaaga
    11641 gcaatacaaa gagttctaca aacatatctc tcatgatttt caggatccct taacctattc
    11701 gcataatcgc gtagaagggc gtagcgagtt tactcaactc ttatttattc ctaagaaggc
    11761 aggttttgat ctctgggatc gcaataagcg taacggtatt aagctctatg taaagcgcgt
    11821 atttattatg gatgacgcag agcagttaat gcctacctac ctgcgttttg tttctggagt
    11881 gattgactca gccgacttgc ctctcaatgt atcccgcgag attttgcaag agtcgcgaga
    11941 tgttaaggcg attcgcgaga gctccacgaa gcgtgtctta ggtatgttgg aggatttggc
    12001 gaatagtgaa gatgcgagca agaagcaaca atatgccgag ttctggcaag agtttggcca
    12061 agtattaaaa gaagggattg gtgaggacag cggtaatcaa gagcgcattg ctaaattagc
    12121 acgttttgct agtacccatt cagactcagc tgagcaaaca gtttctttgg ctgactatgt
    12181 cagtcgtatg aaagagggcc aagacaaaat ctattacgta acaggcgatt cttacactgc
    12241 cgctaagaac agtcctcatc tagagatctt ccgcaagaaa ggtgttgagg ttttacttct
    12301 atccgaccgg gttgatgagt ggatgctttc attcttccca gaatttgagg gtaaatcact
    12361 ggtatccgta gccaagggtg gcttagatct cggcgcgctg gctgatgagg cggagaagaa
    12421 ggagcagaaa gaaacggaag agcaatacaa agacttgatc gagcgtatga agaagacatt
    12481 ggatagcaag gtgaaagatg ttcgtattac attccgtcta actgattccc cagcatgctt
    12541 ggtggcggat gagcatgagc tctccggaaa tttattgcgt atgcttaagg ccgcgggaca
    12601 gcaagcccca gatacgaaac ctattttaga aattaatcct cagcatcctt tggtgctcaa
    12661 actaaaatac gaggacaagc aattcgatga ctgggcaacg attttgtttg atcaagcgct
    12721 ccttgcggag ggcgggcaat tacaagaccc ggctgcattt gtgaagcgga ttaatcagat
    12781 gttattggca taaaggaaac gctatgagag cagtcgtatc tcgttttggt agtggtcatt
    12841 ttcaacaagc tctagaggtt ggatcagatc attttgtttc cgatgtcgat caagaaaagg
    12901 gcggaaattc aaccgggcca tcccctcatg aatacttggg cgcggcttta gccgcttgca
    12961 caggaatgac tttgaagatg tatgcaggta gaaaatcctg ggatcttcag gatgcgatcg
    13021 tgaccgttga tatcctgcga gcagatgaag tggaaatctt caagcgcacc attcaattgc
    13081 ttggcaatat cgatgcggag cagagggatc gcctcctgga aattgccaac aagtgccccg
    13141 ttcataaggc gcttgcaggc aagatcgaga ttcaaacaat cctcaattaa aacgcaccaa
    13201 ataggtgcat ttcaggctag tctcaagccc ctaaacatta ataattagca aaatattaaa
    13261 taaaaggggt aatgatggaa gcggttaaat cgggggcaga tgtcctcttt attatgctgg
    13321 gcgccattat ggtgcttgca atgcacgcag gnnnnnnnnn nnnnaaaatt ttggttgatt
    13381 ttgccgtctc aacgattgca tatttcttta ttggatacag cattgcctat ggtgttcatt
    13441 tcttttcaag cgcagaagtt cttgcacaaa agagtggctt tgagctagtc aagtttttct
    13501 tcttactgac ctttgccgcg gcaatccccg cgattatttc aggggggatc gcagagcggg
    13561 ctaagttcaa cccacaactg attgccacat ttgtaatcgt tggatttgta tatcccttct
    13621 ttgagggtat tacttggaac cagcattacg gaattcaggc atggatcaaa gagctaacgg
    13681 gtgatgagtt ccatgacttt gcaggttcga ttgttgtgca tgcagtgggt ggttggttag
    13741 ctttgcccgc catccttttg ttgggtgcgc gacgtggtcg atattccaag gaggggcaaa
    13801 ttgcagccca tccaccatca agcattcctt tcttggcctt aggcgcatgg attctgaccg
    13861 tgggttggtt tggatttaat gtgatgagtg cacagaccat tgataaggtg agtggcttgg
    13921 tggcacttaa ctcattaatg gctatggttg gtggaacctt ggcagcctgg ttggtgggcc
    13981 gcaatgatcc tgggttttct tataacgggc ctttagcagg attggtggca gtctgtgcgg
    14041 gctctgatct tatgcatccc ttgggagcct tagtgacggg attggttgca ggcgcgttgt
    14101 ttgtctacat gtttacccaa gtgcaaaatc ggtggaagat tgatgatgtt ctaggagttt
    14161 ggcccttgca tggcctctgc ggattatggg gcggtctagc agctggtatc tttggcgcca
    14221 cagcccttgg cggtcttggc ggtgttaacc ttgccgccca aacaattggt agcctattgg
    14281 gagtagccgt tgctctagta ggcggctttg tggtctatgg aatcttaaaa atgaccctcg
    14341 gtattcggat gtcacaagag gatgagtacg agggcgctga tctgagtttg cacaaaattt
    14401 ctgcgactcc agaccgagac tccagttggt agcctagcct cactataatt ggggcatggc
    14461 tatatatgaa ttagatggca tttcccctga attaggcgat ggagcatggg tggcagactc
    14521 ggccgaggtg atcggtcgag tgcatctagg caagaatgct aatgtctggc cacaggtggt
    14581 aatccgtggc gataacgagc cgatacgcat tggtgctggt accaatattc aagatgcagc
    14641 agttttgcac tccgattttg gctcgccatt aaccttgggc gagaacgtat cgataggcca
    14701 ccaagccatg gtgcacggtt gcacgattgg cgatggtagt ttgattggga ttaatgccat
    14761 tgttcttaat ggtgccaaga tcggtaagaa ctgcttagtg ggtgctggtg ctttagtcac
    14821 cgaaggtaaa gagtttccgg atggctctct cattattggt tcaccggcta aggcagtgaa
    14881 gagtctaagt ccagaacaaa ttgctgagat agacagaatc tcatcccact atgttgagaa
    14941 tgcaaagcgc tatcgcaacg gtctaaagaa acttgcatag gctttagttg ctgcacgttc
    15001 ttaacgtcat catcccgatc ttcgctttaa tactagcggg atatattgct ggtaagtctg
    15061 gcaagttaag cgttaatgca tcgactgaaa ttaatcgctt tgtggtttgg ttggcactgc
    15121 cagcgcagat gtttgatttc acggcccaca gtaattggca agagttatgg caacctggtt
    15181 tcatcatcgc ttttagtgcc ggtgcactag ctgtttattt cgcactctta atttactacc
    15241 gcattcaaac caaagattgg accgtagcca gctttcagag cttgagtggt tcgtatgcaa
    15301 acacaggcta tatgggcatc cctttgttga tgctcgcttt tggagagagt ggattgggcc
    15361 ccgctgtcat tgctactttg atagtggtct gtgctttatt tgccgtagcg attattttta
    15421 ttgagttagg ggtgcatgct aaaaaaccct ttagcgagat cattcaaacg gttgctaaat
    15481 ccttggcaac taatccatta ttagtggccc cgttttgtgg agccctttgg gcaaccaccg
    15541 gcttagagtt gtatgcacca ttgcgtcagt ttgtgagttt cttgggtgca gcggctagtc
    15601 catgtgctct ggtctcaatt ggacttttcc ttgttcaaaa aacgagcgct ccaactacga
    15661 caacgtggag tttggtcttc gttaaattaa tcctccaacc gtttgtggtg tgggtggtag
    15721 ctgatccaat attgggctta ccgacctttt gggtatacgc cgcagtttta gtgagcgccc
    15781 taccaacagg gacaggcccc tttatgttgg cgcaatacta tcgcgcggat ggctcactca
    15841 tttctagagt ggtattgatt actaccttgg gatctgtcat taccttatcc gctttagtgt
    15901 ggtggatgac ccctcactag atctgaggat ttgatccttc ggcatccaat tgtttctccc
    15961 aagacgcatt gataaatgca ggcagggtca tgcaatgggt gacgatgcga tgaactgttg
    16021 gatagacact aaaatcgatc ttggcgtcat aagcattaaa gacctgcgga actaaaaaga
    16081 catctgccat gcttggcgtg tcaccataac aaaattgacc aacacgagca tccttcgcaa
    16141 gacgtttttc tagactagcc agaccttttt taacccaatg gttataccaa atggttttgt
    16201 tctcatcgct cactcctaag gtacctacga ggtagcgtag tactcgtaaa ttattaatgg
    16261 ggtgaatatc ggctgcgata tcttgtgtta gagcccgaac ccatgctcga tcctctaaat
    16321 ttttggggag cagtgatgat gggttggggt atttctcttc tagatactca atgatggctg
    16381 ctgattgatg taagggaaca ccattctcga tataaagggg caccaggcca ttcgggttaa
    16441 gagcttgata gggctccttg agttgttccc cgccaccctt acgtaaatgc acggggatga
    16501 tgtcgtaatc gatcccttta aggtttaagg caatacgaac ccgaaaggca gcggagctgc
    16561 gccagaagct ataaagaatt ggtttcatta gaaggatgct accgcaccat cgctgcgcgg
    16621 atcccagccg ccgcgcaaga tcccagatgg ctcccgaata atgcaacccg catgacccac
    16681 cgattcatca aagtcagcga gcatctctac atcatgaccc atttctttga gttgcttcac
    16741 tagagaggga tcaaagcgtg attcgagctt cagactatcg ctggtttgcc cccaagtacg
    16801 accaagcaac caacggggcc gactaatcgc atcttgtggg tctagcccat agaccgctgt
    16861 acgagtgaat accgcacact gcgtttgtgg ttgtccgtca ccgcccatgg tgccatatac
    16921 catcgagcga ccatctttaa ataatgccag tgcaggatta agtgtatgaa atggtttgcg
    16981 atagggctct agggcgttga gtgcattggc atctaaagag aaactgcaac cgcggttttg
    17041 ccaattaatc cctgttttag gtaaaacaat accagctcca aactcgtgat aaatactttg
    17101 aatgaacgag acgcagcgac cctcaccatc aatgaccccc atccaaatcg tatcagcagg
    17161 cccacgccca gcaccccagg gcaatgcttt ctgactgctt accttatcag caagagtctt
    17221 taagaaggcg ggagataaaa attcttgggc atgctgtttc atataagcag gatcggtaac
    17281 aaattgatca cgaaccttga atgcttgctt ggttgcctca acacagtgat gaacatactc
    17341 tgggctatct accgcgtatt gccttaaatt gagttgatcg aggatgccca aaatcattaa
    17401 tgagaccacg ccttgcgttg gcggggtcat gttgtatacg tcagcaacac tatgagcaag
    17461 atgcaaagga ttaatcagtt ttgcctggtg acgatttagg tcatcaagcc gtaatggact
    17521 accaatttgt ttgagttctt tggcgatctg ttcagcaagc tcaccgcgat agtaatcatc
    17581 cgttcccttt ttggcgatct ggcgcagggt tgccgctaag cgtttttgta gaaagacgct
    17641 accggtggcg ggggccttgc cgtttaccaa gaaggtgtct gcaaaaccag gttgcggagt
    17701 taactcagcg cgcttctttt ctgttagggc agactggcta tgggtaacag gtacaccacg
    17761 ctctgcataa tggattgcat cttctagcaa gcgcgagagc ggcagtctgc cacctaaact
    17821 ctttttagag agagcgaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnggtaat gccttgagat tgataccaag agcgcgttgc taaaccagca
    17941 gcagccccac aggcatcaat gccccccatg gctttgccag gagcatgaat cacccaaaac
    18001 gagtcgcctc caatcgagtt catatggggg tagaccactg caatggtagc tgcagcggcg
    18061 atcatcgcct ctaggctatt gcccccctcc cgcaaaacag cgagtgcaga ttgagaagcg
    18121 agtgagtggg gcgcaacagc catcccctga ataccccatt ttggttgcat gcaaacctca
    18181 gaaaataatt aaagcgctat tttatttgtt ctctttgagc atcttctcaa tatctgcact
    18241 tgagtcaggg ggcgcttgag caggagcact gctcccttgt agtggatcga tcaggatatc
    18301 tgaactagat tcaatagcta aagtaggttt atcaatgaaa taagttacgg tctcgggtac
    18361 aaaaataata atagcaacca aaataagttg cataccaacc caagggaggg ctccccagta
    18421 aatatctgaa ctcttgacag atttatctgc aactcctctg aggtagaaaa gcgcaaatcc
    18481 aaatggcgga tgcataaaag aggtttgtaa atttgcacca agcaaaacac caaaccaaat
    18541 taaatcaatt cctaattttt cagcgactgg agccaatagc ggaataatga tgaaggcaat
    18601 ctcaaaataa tccaagaaaa atgccaagaa aaatacaaag agattaactg caattaaaaa
    18661 accaatttgc ccaccgggta aagaagtgag taattcttca atccaaagat ctccatccac
    18721 acctctgaac gtaagtccaa atactgttga tccgattaaa ataaaaatga ccattgcatt
    18781 gattcgcata gtggatgtca ttgcttgcca gaccagatct ttttcaagac gcttattaat
    18841 tgcggccaaa gctaatgcgc caaccgcccc cattgcgcca ccctctgttg gtgtggctag
    18901 gcccatcaag atggtgccta gaaccaagaa gatgagcact agtgatggaa caatgcccca
    18961 cagaattcgt ttaaaaagtt tccaatcaat cggcgggcgt gcttctttag gcaaggccgg
    19021 aacatattca ggtttgatga ttgaaaccat aaaaatgaac agacaaaaga gtaatacttg
    19081 gataattgat gggccaatag cgcctgcata catatcgccg accgatttgc ctaattgatc
    19141 agccaaaata accaaaacta aagatggcgg aattaattgc gtaatagttc cggatgctgc
    19201 aatgacaccg gttgctatac gcgggttata accatagcgc aacataatgg gtaaggagat
    19261 tagacccatc gcaatcacag atgctgctac ggtacctgta attgctccca aaactgcacc
    19321 taccagaata actgcgtagg ctagcccacc acgtatgggc ccaaatagtt gccctaagcc
    19381 ttctagtaag tcctccgcca aaccgcaacg ctctaatatg gcgcccataa aagtaaagaa
    19441 aggaattgcc aaaagaagat catttgagag aattccaaaa acacgatctg gtaatgcttg
    19501 caaaaagctt ggttgaaaaa aaccaagttg aataccaaaa acagcaaaaa agagaccaac
    19561 agcgctaagt gaaaaggccg cgggatatcc aattaataaa aaaataatta gacccccaaa
    19621 cattaaaggg gccatgatgt cgtggctaat cattgcaggg gtctttcgta cttaggatct
    19681 atttggatga tgccttgtag tgcagcatag cgtttgatga tctcggaaat tccttgaaga
    19741 aaaagtaaga aaaatccaat tgggatcatg attcgcgcgg gccaacgaag aagcccgcct
    19801 gcattgctcg atatttcatt ttgaaggaaa gagttgaaaa ataattcaag cgaattccag
    19861 ccgatgatta ggcacatggg taataaaaat aagatgcccc cccagaaatc gagccagagt
    19921 ctgactcgat tggggtacat ggcatagagc acatcaacac gcacatgttc attgaggcgg
    19981 aacgttgtag ctgcaccaag catgaccata ccggtaaaga gataccattg aatttctagc
    20041 caagcattgg agctaatact aaagctgtag cgaataattg cattaccggc gcttattaga
    20101 accgcaagca acactaacca actagccagc acgccaaaac ggtcattcaa ttgatcaata
    20161 aaagaagaaa attgtaaaaa ttgttttggc atgatggcag tcgtttattt cactgggtta
    20221 gaaagcatga aatcaatcaa ggttttttct gctactcgcg tccataagaa ttgctccgca
    20281 cggaatttct tccaaggttc ataaatcttt ttaaaggaag gattttttgc cgactcttct
    20341 tcgtaaagat caaatgctgc ggtttgggct gctttcatga tttcgtttga gtaagcatgc
    20401 aacttgactc cattctttac caagcgttgc agtgctggtg ggtttttgaa gtcatactca
    20461 gccatcatgt caagattcgt ctcatagcaa gcaacttcaa gtgcagattg atattgctta
    20521 ggaagttttt cccattcttt agtattcaca tagagggagt acatnnnntc ccaccaacct
    20581 gggtagtagt aattaggcgc aattttatag aagcctaatt tctcatcatc atagggaccg
    20641 acccactcgg ctgcatcaat cacgccgcgc tctaaggcag gataaatatc accacctggg
    20701 atctgttgtg gaatcgcacc aagacgagcc atcacttcac cgccaagacc agcaatacgc
    20761 attttgagac cctttaaatc agcaacggac ttcacaggtt ttttaaacca tcctcccatt
    20821 tgtgttccag tgttaccagc cgggaacgag ataatgttgt agtcctttaa aaactctcgt
    20881 tgtaatttga gaccgccgcc ccaatacatc catgcatttt gttg
//
LOCUS       DDPQ01000053           11279 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_15518,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000053 DDPQ01000000
VERSION     DDPQ01000053.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11279)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11279
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_15518"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3368..3457
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7659..7754
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8278..8336
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8465..8513
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9457..9474
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2911 a         2766 c         2500 g         2786 t
ORIGIN      
        1 atcacctgac cacccatttg cacatcacca gaggtggcag tatccaatcc gcctagcaaa
       61 tgtaataagg tactcttccc tgatcccgag gagcccacaa tcgccacttt ctcagcgcga
      121 tctaactcca gatcaacaga ctttaggacc tctactgcgg tcgctccagc gccataggtt
      181 ttggcaagtc ctgtagcacg taatacagga ttagtggatt tattcatagc gcaaggcctc
      241 ggctggcttt acctgagccg ctcttctact gggataaata gtagccagaa tggagagaac
      301 aaaagccatc acaccgactg tgagtacatc atcaaaacga acatccgatg gcaactggct
      361 aatgaaataa atatcacggg gtaagaactg aacccgaaag agagcttcaa tgaacggaac
      421 aatcacatca atattaagcg ctattaataa accaaatacc acgcccgata gactacccaa
      481 tagtccaatt gccaatccct gaactaaaaa gatgcgttgg accaaaccag cacttgctcc
      541 catggttcgt aagatcgcaa tatcagcttg tttatcggtt acggtcatca cgagggtgga
      601 aacaagatta aaggcagcga ccgcgatgat taaggtcaga ataatgaaca tcattttttt
      661 ctcggtctga actgctgcaa accaattgcg attggagcgc gaccaatcac tcacccataa
      721 tgcttgaggt accacctgcg ctaatttcac tgcgatctct ggcgctcttt gcatatcatc
      781 gaccttcacc cgtaatcccg ttggatcgga catgcgcaac agtgcggcgg catctcgcca
      841 atgcatgatg gtcaatgtac tgtcatattc ataatgacca ctatcaataa tgccaacgac
      901 ctgcaaagcc cgcatgcgcg gcatcatgcc cgctggggtt aaatcgcctt cgggaactaa
      961 taggctcact cgatcaccaa tgcgcactcc aagcgtgccc gccaattggg cgcctagcac
     1021 cacaccaaat tggcccggct ttaaatcatt catcgagccc gccacaaatt gtttgggaag
     1081 atcagagacc ttgccctcca gctctggggc aacgccacga agagcgacgc ctcgcatgga
     1141 ctcgccacgc gataagagcc cttgggaact caccatgggt gccacgccaa gaacgtgggc
     1201 ttggcccgat agtttctccg ataccgattc ccagttggct aaaccagcag gtgaagtgat
     1261 ttcaatatga gaaagtacgg agagcatccg atcacgcact tctttttgaa agccgttcat
     1321 caccgagagc accacaatta aggcagcaac gcccaaggca atccccgcca tcgagatccc
     1381 tgagataaag gataaaaagc cgtcgcgttg gcccacagcc ttacgccgtc tagagcgcgt
     1441 gtactttagg ccaatctcca gttcaatcgg aatcttccac atacccacag tttagtgaat
     1501 cacaagcccc tactgggcaa ctctcgcaaa tgccacctaa aattgagggt atgcctaaac
     1561 atctcacgct tttgcttcat ggcgaaaaca aactcgatga cgagtctatt gccttagtgg
     1621 gcaatggctt agcccatgag caataccttc tgggtgagtc atttcctctg gctggagctc
     1681 tggcaggccc agaatccgac aatctccaac gcatctgcat tgagccagtt catcttcatg
     1741 caacccgcga tcatctcgtg ctgcttcaac ttgatccttt tggggtcact caagaagatc
     1801 tttcacaact gatgaacgaa gccagtacta tttttagtga agaaggtttg gggcctctga
     1861 cacaagtcgc gccctttcag tggttaggta aaaccgaggg atttaaaaca ctgcatacgc
     1921 atagtgctgc gcaagcacaa ggtcgcaaca ttgactggtg gctacccaaa gatacacgag
     1981 tagtcggttt agccaaacgc tggcgccaaa tacaaaacga agtccaaatg cgctggcata
     2041 ttcatcccat caatcaagcg agaagtgagc agggactgcc caccattaat tccatttggc
     2101 taagcggcat tagtcatcaa cacgatgtgc atttggccca cgaactcaag atcgcccaaa
     2161 aaatcattag tgatcaagcg tggatgcctc gtgtggcaca aaatcattcc ttagtttgca
     2221 tgagcgaccc tatctcccgc tgggaggatc tcgcggagaa caccttgatt taccatcgtc
     2281 aggcaaaaca gatgtggcct gccatttatc agatgttgct cgatcacgat ctagtcctgg
     2341 aggtgattga ttttccaaaa tcagttcgta agcgtcgctt tgcctcggac gattttcgcg
     2401 ctcacccgct cgccttttgg cgaaaatcca aagttcccac atgggaagag ctcaacgcgt
     2461 gagtatcttt agccaaaggc cgtattcgtc agagattgcg aagactcttg cgtcaggtga
     2521 gttacatccc ctcttagcaa gactctacgc cgcacggggt gtagcaagtg ttgatgaact
     2581 tggcttagag ctcaagtatc tcttaccacc caataccctc ttacattgcg aggatgccgc
     2641 caagatcttg gccgatgcaa tacgtgatca acgtcagatg ctgattgtcg ccgactatga
     2701 ttgcgacggt gccacagcct gcgctgtggg tattcgtggt ttaaagatgc ttggtgcatc
     2761 acccaagcaa attgattttt tagtgcccaa ccgctttacg atgggatacg gcttaacgcc
     2821 tgaggtgatc gatctagcaa aagagcaaac ccccaaaccc tctttactca ttacggtcga
     2881 taacggaatt gccagtcatg caggaattga ctatgccaaa gagcatggca tggatgtgct
     2941 cgtgaccgat catcatttac caagcgagca attacccaat gccctagcca ttgtgaaccc
     3001 caatcaaccc caatgtggtt ttcagagtaa agcgcttgcc ggtgttgggg tcatgttcta
     3061 tctcttaatt gctctgcgcg cagagctgcg ccaacgcggc atctttacaa cagagaccca
     3121 acctaagctc gaacagttat tagggcttgt ggctcttggc accgttgcgg atgttgcgca
     3181 actggatcgc aataaccgca ttttggtatc acaaggtcta aagagaatcc gccaaggtat
     3241 tacgccgcct ggacttcttg ccttgtttga ggtcgctggg cgcaatcccc aaacggcaaa
     3301 cccgtttgat ctggggtttg ccgtggggcc aagactcaat gccgcaggac gtcttgccga
     3361 tatgagtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta gaccggatta atcgtgagcg
     3481 ccgggtaatt gaatcgggaa tgcaggagtc tgccctagcg cacctcacgc accttaatac
     3541 ggatgaacga ccagcgcttt gtttatggaa tccgaattgg catcaaggag tggttggtat
     3601 tgtggcttct cgtctaaagg agcgctttaa tcgtcccgcg attatttttg ctccggcaga
     3661 gggggttgat ggtgaagcgc ttttgcgagg atcggggcgc tccattgctg gatttcattt
     3721 acgggatgct ttggatctgg tctctaagcg caaaccttct ctcattcaaa agtttggagg
     3781 gcatgctatg gctgcaggtc ttacgattcg gcaggccgat tttgggttct ttgaagaatc
     3841 ctttttacag gtcagtcgtg agctcttaaa cgacgatctt ctccaacggc gctgcatcca
     3901 tgatggggaa ctaagccctg aagaattaaa tgtgcagacc ggcgacatcc tcatgaatga
     3961 gatttggggt catggctttc cagagcctct tttttatgga gagtttgatg ttctccagca
     4021 atccctcatg aaggataagc acttacgctt acaactaaaa ccaaggggcc tcgattcgga
     4081 aacggtaggg catgcgccgg ttattggggc ggtctggttt aaccgcaccc aaacccttcc
     4141 cccaaaagct cgtctggcct atcgtttagt cagcgatcga tttcatgcag aggcgcgcgt
     4201 gcagttaatg attgaggcgc tcaacgatca agcctagttg gcacttaccc gtataatttg
     4261 ggtatggaag ccgaacaact caatcaaatt acgaatgcct taaaagatct tctctcgcgt
     4321 gagcaagcac tacgggggta tctttgactt caatacgaag tcaaaccgtc ttgtagaagt
     4381 taatcagatc cttgaagatc ccaaaatctg ggatgatccc aagcaagctc aagcgctggg
     4441 taaggagaaa aaattacttg agggtgttgt caatacccta acagagctca atcaaaacat
     4501 cacaagcagt atggagctca tggaattagc acgtgccgag aacgatcaag agaccttact
     4561 agctcttcaa gctgactcgg attcttatga aaaaatcatt gcggatttag agtttagaag
     4621 aatgtttcac aatgagatgg atccctgtca ttgctttatt gatatnnnnc aatatctcaa
     4681 atactgcgag cgtaaaggtt ataagactga aatcctagaa gaatctgatg gtgatgtcgc
     4741 tggtattaaa agtgccacca ttaaagtcga tggtgaatac gcatacgggc acctgcgctc
     4801 tgagacgggg gttcatcgtt tggtacgcaa atctcctttt gattcgtcag gtggtcgaca
     4861 tacttccttt gccagcattt atgtctatcc agagatcgat gactcgattg aaattgatgt
     4921 taatcctgct gatatccgca ccgataccta ccgtgcatcc ggagcaggtg gccaacatat
     4981 caataaaaca gattcagcag tgcgtcttac acatattcct acggggattg tggtgcagtg
     5041 ccaaaatgat cgcagccaac accgcaatcg cgctgaggcg atgagtatgc tcaaatcccg
     5101 tttgtacgaa cacgagatgc gtaagcgtcg tgctgagcaa gataagttag aggccaataa
     5161 aactgatgta ggctggggtc atcaaattcg atcctatgtt ttggatcaaa gtcgcattaa
     5221 agatttaaga accaatgttg aaatatctaa cacccaaaaa gttttagatg gggatctcga
     5281 tgcatttatc gaggccagtc taaaacaagg tgtgtaatat ccagagagct atccctatga
     5341 ataccactgt acctaatgct gatgagcaaa accaagcgcc ggatgagaac cacatcattg
     5401 ccgagcggcg cgagaaatta gccaaacttc gccagtctgg cataccgttt cccaatgact
     5461 ttatcccaac gcatttagca gcagatctgc atgatcatta cgatggcttt agtaaagaag
     5521 aattagcaag caaaaagatt ggcgttaagg tcgccggtcg aatggtatta aaacgggtga
     5581 tgggtaaagc tagttttgcc acgattcaag atcgtagcgg tcagatccag ttctatatca
     5641 gcgacgacct aacaggcacc gatgtccatg cagcctttaa acattgggat ctcggtgaca
     5701 ttattgccgc cgagggtcat ctctttaaga ccaacaaagg cgagctctca gtcgagtgct
     5761 ccacactacg cctactgagc aaatcgttgc gaccactgcc agataagttt catggtctgt
     5821 cggatcaaga actcaaatac cggcaacgct atgtcgattt aattgtgaac ccagaaagcc
     5881 gtaatacttt cttggcacgt agcaaagcca ttgcctcgct gcgtcgacat atgatcgaag
     5941 cacagttcat ggaagttgaa acacccatgt tgcaccctat tcctggggga gcagcagcta
     6001 agccattcat cacccaccac aatgcgctcg atatgcaaat gtatatgcgg atcgctccag
     6061 agctttatct aaagcgtctg gtagttggtg gctttgagcg ggtctttgag attaatcgta
     6121 acttcagaaa tgaaggtctt agcccgcgcc ataatccaga gttcaccatg atggagttct
     6181 atgcggccta taccgactat cgctggctga tggactttac cgagaattta atccgcagcg
     6241 ccgccatcga tgcgcaaggg acagcaacac tcactcacca agggcgaggt cttgatttag
     6301 ctaaaccctt tgatcgtctc accattaccg aagcaatcct caaatacgcc ccactctcgc
     6361 aaaagaccta tgctgcggcc caattagaag accttgactt tattcgtgcc gagctaaaaa
     6421 agggaggaga aaatattaac tccccagcac tcaaaaatgc ggggctaggc gctctgcaat
     6481 tggctttatt tgagctcgtt gccgaagagc atctctggga tcctacttac attattgatt
     6541 atccgattga ggttagcccc ctcgcgcgtg aatcggatac tcgcaagggt gttaccgagc
     6601 gctttgagct ctttatcaca ggtcgagaaa tcgccaatgg cttctcagag ctcaatgatg
     6661 ctgaagatca ggccgaacgt tttcgcaagc aagtcgcaca gaaagaggcg ggcgatgaag
     6721 aggcgatgta ctttgaccat gatttcatcc gcgccttgga atatggcatg ccgccaacag
     6781 ggggttgcgg aattggtatt gaccgcttag tgatgctact aactgatatg cctaatattc
     6841 gggatgtgat tttgtttccg catttgcgtc gcgaagaaga gtgatcgtct agcgattacg
     6901 cacgcggtcg cttttagtaa cccgctcata aaatgcattc ggtttagtct caacccagcg
     6961 aataaatttt tgcatcgcgg gatcttgcaa aattgcctct gccgtattga atttagcggc
     7021 taactgcgta tcggtaaaga gcgcatgaat ctgtcgatgg caaatcttgt gtaaatactc
     7081 agtggctctg ccgccctttg atttcggaat caaatgatgg gcttcttgtt ggtctggcgg
     7141 tatctcacgc atgcaaatcg ggcaaattgt aagcgcaacg gcattgagtt cctcgcttgc
     7201 ttgaagttct agcaattgct tttgacgtat ccgaccgacc atggtgctta attagaatta
     7261 cttagtgatt ttctttggca tgattgatcg aatactttgg aatctcaatc actaaatcgg
     7321 ttttagagac aatggcctga cacgaaagtc gcgattgggg gcataagccc caagcacgat
     7381 cgagcatatc ttcctcgttc tcatcgggct catttaagct atttcccccc tctcgcacaa
     7441 tcacatgaca ggttgtgcag gcacacacca tatcgcaggc atgctcaatc tcaatatcat
     7501 tagcgagcaa ggtctcgcaa atgctagtgc ctggatctgc ttcaatgacc gctccgtcag
     7561 ggcaatactc agaatggggt agaacaacaa tttgggtcat gggtcagtct cgataattag
     7621 atctctgcta cattttttcc ggtcaaggct ttctgaatnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnncggagc aaactcattt ctttacgaat tagagcatgc tcaacatcgg
     7801 ttaataaatg tctgtcttgc tctaaagcag ctcctacagc ttcgagtaaa cgctctgcct
     7861 ctacctgctc ttcgcgcaaa gagcgagctt gcatatcgtc cttggcagac gcaaatccgt
     7921 cttgcagcat ccgagtaatg tctgtatcgg taagaccata agacggtttg atctcgatcg
     7981 aggcttgcac ccccgagctt tgctctaatg catgaaccga taaaagaccg tcggcatcga
     8041 cctgaaaggt cacccgaata cgagctgcgc cggccaccat cggtggtatc ccctttaact
     8101 caaaacgcgc caaagatcga cacgcatcaa ccacttcgcg ttcaccctga acaatatgaa
     8161 gcgataaacc ggtctggccg tctttgaagg tcgtgaactc ctgtgcctga gccactggga
     8221 tgggggtgtt acgtggaatg attttttcta ctaagccacc catggtctca agccctannn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcac
     8341 ccagagcaac cacttgatca ggatttaaat tatttaaggg ttgctttgca aagagatctg
     8401 ctacagcagc ttgtacatgg ggcatccgcg tcgcgccgcc aaccatgact acgcccttaa
     8461 cttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgactc
     8521 acttccacat gaacgacggt acccgtactc agagtgaggt gcacactcgc aagcggagca
     8581 tgacttaatt gttctttggt ttgtttagct gctagcaata aagcacgttg gtcttggggt
     8641 gcaagaggtg gcaacttagc ttgcgcaatc acccagccat ataggtgttg atcaaaatca
     8701 tcgccaccca aggcagaatc acccccggtg gataagacct caaagacacc ctgactcatt
     8761 cgtaaaatag agatatcaaa tgtgccaccg cccaaatcat aaacagcgta gattccttcc
     8821 gaggcattat ctaaaccata ggcaatcgcc gctgctgttg gctcatttaa taatcgcaag
     8881 accttaaggc ctgctagttg ggcagcatct ttggtcgctt gacgctgggc atcatcaaaa
     8941 tatgcgggga ctgtaatcac cgctccgacg atctcatctt ggacggagtc ttcagccagt
     9001 tgacgtaagc gcgctaggat ctgtgcacta atctctaccg gagttttatc tcctgcgact
     9061 gtgcgcaact tgaccatacc tggctcatcg ataaactcat agggagaact ctccacatgc
     9121 gatacatcat gcaggccacg tcccataaaa cgcttaaccg aaataatcgt atttttagga
     9181 tcatcgatta catgctctaa ggcctcataa ccgccctgag ttcgaccgcc tggtaaatac
     9241 cgaatcaccg atggaaatag ttccttgcca ttaatatcgg ctagaacctt aggaagcgca
     9301 tctttgacaa tggcaacgag tgagttggtg gtgcccagat caataccaat agcgattctg
     9361 cgctcgtgcg gagcaagagc cattccaggt tcagcgattt gcagtaaggc catcgtattt
     9421 ttctatataa atttaagcca gctcagcaat cgcatcnnnn nnnnnnnnnn nnnntcgcac
     9481 tcttcttcta aaagagtgac agcattttgg tgggcttgac ccacttcggc tgccaaagtc
     9541 tcgagggtgc ttaagtcctc tgcagactca tcgagggtct cacgccattc catttgcttc
     9601 attagaaatt gaacgggcat ggccgtattg gtttctaaat gcgcttctac gccatgtaac
     9661 tggcaaagat ataagcctct ttggatagga tcttttaatg tgcgataggc ggtgttcgca
     9721 taagtcgcta attgcattgc gacccgttgc tctgcttcag ttccatgagc gtgacgatcg
     9781 ggatgaactt ctttttgtat agctaaatag gcttggtcca aaaccaacgt atcaagcttg
     9841 aaacggggtg ttagtccaaa aaaagtgaag tagttgtcag acgcggaaag attcgccaca
     9901 accacactca tctttcacat tggggttctg aaacttaaac ccttcattta atccttcgcg
     9961 cacaaaatca agctcggtgc catcaatata ggcgaggctc ttcggatcaa caaagatctt
    10021 aataccgtgg gactcaaata cttgatcctc tgcgacagcc tcatccacat actctaattg
    10081 ataggcaagg ccagaacaac ccgttgtgcg aacacccaag cgcaagccac aacccttacc
    10141 gcgtttttgc aaactacgat ttacatgagt cgctgctttt tcggtcaggg taatcgacat
    10201 atcaatctac tttattaatt ggaatgcttg tctttgtaat ctttcactgc cgccttaata
    10261 gcatcctctg ccaaaattga acagtggatc tttacgggtg gcaaggaaag ctcttgggca
    10321 atctgcgagt tcttaatctc gagcgcttga tccaaggttt taccctttac ccactcggtc
    10381 accaaggagg aagaggcaat cgccgatccg caaccatagg ttttgaactt agcatcttca
    10441 atcacacctt gatcgttgac ccgaatttgc aacttcatca cgtcaccaca tgcgggtgcg
    10501 ccgaccatac cggtaccaat ttgctcatcg cccttggcaa atgaacccac attacgaggg
    10561 ttctcataat gatcaattac tttatcgcta tatgccatgt cgttaccttt ttaatctcaa
    10621 tacatgaatg attaatgcgc tgcccactga atcgtgttta ggtcaacgcc atctttgtac
    10681 atttcccata aaggagaaag ctctcgcaat ttggcaattt tctctttcac taaatgaatg
    10741 gtgaagtcca cttcttcttc attggtaaag cgtccgatcg taaaacgaat cgagctatgg
    10801 gctaactcat cattacgtcc aagtgcacgc aatacataag aaggttctag cgatgcggat
    10861 gtgcatgcag atccagaaga gatcgcaatg tctttcaatg ccatcagcat ggactcgcct
    10921 tcaacatagt taaaactgat gttgaggtta tgcgctacac gcttgtccat atcaccattg
    10981 acataaacct cttcaatgtc ttttaaacct gctaacaaac gatcgcgtaa tttacgaata
    11041 cgggcgctct cctcaaccat ctccactcga gctatccgaa acgcctcgcc cataccaaca
    11101 atttgatgga caggtaaggt gcctgagcgc atgccacgct catgaccacc gccatggatt
    11161 tgcgcctcaa tgcgcactcg gggtttacgg cgaacataca atgcaccaat gcccttgggg
    11221 ccataggttt tgtgtgccga gaagctcatc agatcgactt tttgcttctc taaatcaat
//
LOCUS       DDPQ01000054           32545 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_707, whole
            genome shotgun sequence.
ACCESSION   DDPQ01000054 DDPQ01000000
VERSION     DDPQ01000054.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 32545)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 32545)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32545
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_707"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    14560..14628
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22725..22778
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    28433..28512
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8630 a         7964 c         7175 g         8571 t
ORIGIN      
        1 ttcatgcaac gtcttgggcc atccatattg ggcgcagtca tatggtatgc atcaccactc
       61 atcccaaaac cacataactc tgcatagatt tttgctccac gagcacgagc atgctcatac
      121 tcttctagca ccatcacacc ggcaccctca ccgagaacaa acccatcgcg gtctttgtcc
      181 caaggacgtg aagcagtcgc aggatcatca ttgcgagttg agagtgcacg agctgctgca
      241 aaaccaccca ctcctaatgg cgaaatagtc gactctgcgc caccagcaac catcacatca
      301 gcatccccat attgaattaa ccgagccgcc aaaccaatgc tatgcaaacc ggtcgtgcaa
      361 gcggttaccg ctgctacatt aggacccttg aggttaaaca aaatactgag atgacctgag
      421 atcatattaa tgatcgatcc aggtacaaag aagggggaga tgcgtcgtgg gccacgcgag
      481 agtaactctg acttagtatc ttcgatcatg ggaaggccgc cgataccaga gccaaccatt
      541 actccaatac gttccgcatt ttgatcgcta atcgtgatac cactgtcacg aattgcttgg
      601 gtgccagcag caatgccata gtggataaaa gtatccatat ggcgcgcctc tttagcagag
      661 acgtattcct cgacattaaa gtcttttact tccccagcaa agtgaacact tagtgcagag
      721 tgatcaaact ttgttatggt ggcaatacca gtacgacccg caatgaggtt atcccaagcg
      781 gtcgcaactg aattgcctac tggtgaaatc agacctaagc cagtgaccac cacgcgacgt
      841 ggttgtttta atgcgggcac gacttggcct aaataatgat attagccctg tccagctttc
      901 gttttagcga aatcaatcgc aagctgaaca gtggtgattt tttcggcttc ttcgtctggg
      961 atttcaatac caaactcatc ttctaaagcc atgaccaatt caacagtgtc aagagagtct
     1021 gcgccgaggt cattcacaaa agaagactca ttcttaatgt ctgcttctgg cacgcctaat
     1081 tgctcagcca caattttctt aacgcgttgt tcgatgttgt ccattcattc cctccggggc
     1141 ttgttaaaac gaattacagg attttatcag tttgttagcc gatgtctcaa tttactagaa
     1201 taattaagct aaatataggc cgccattgac gtgcagggtc gttccggtga tataggcagc
     1261 ggctggggag gccaaaaatg ccactgcatg agccacatcc tcaggggtgc ctagacgggc
     1321 taagggaatg tttgccttaa gactattttg ctgctcttca ctcagcgcac gcgtcatatc
     1381 cgtgtcaata aagccgggag caacgcaatt tacggtgata ttgcggctac caatctcgcg
     1441 cgctaaggca cgagtcattc ccgctacacc tgcttttgca gcggcgtaat ttgcctgtcc
     1501 agcattaccc atatgtccga caatcgaagt gatattaatg atgcggccac ttcgtgcctt
     1561 catcataggt cgcataaccg cttgcgaaag tttaaataca gaacttaagt tagtagaaat
     1621 gacatcggtc cactcatcac ccttcatccg cattgccaaa ttatctttag taatccccgc
     1681 attgttgacc aaaatgtcga tgcgaccaaa ttcttgaaca atgctctcaa taatgctttc
     1741 acacgctgcg ttatcggtga catttaaaac cttagccgta ccgccatgcg cttgcaggcg
     1801 cgctgcaatc gcttgtgcgc ctgactctgt ggttgccgtt ccaataaccg ttgcgccgca
     1861 cgcggcaagt tcttgcgcaa tcgcttggcc aatacctcgg gatgctcctg ttactaaagc
     1921 aatttgattt ttaagatcta aattcatgct tgtctcttta ataggtctac gattttaatg
     1981 tttgcatgac ttccatgagg ctggcctcat ccatcaatgc cataccctgc aattgatcgt
     2041 caattcgttt cgttaagcca gctaatacct taccagggcc acactcaacc acctgaccca
     2101 cgccacgcgc tgacattgcc cgaattgttt cttgccaacg tactggtttg gcagcctggc
     2161 gcaccaaggc gtccttgatg gcagctggat tatccaaaat actcacgtct acattgttaa
     2221 ttacaggaat ggtaggaaca tgaaactcaa tattctcaag gtatgtcttt aacttctcag
     2281 acgcaggctt gagcaaggag gaatgaaatg gggcggatac cggtaagggt aaggcacgtt
     2341 tagctccggc tgcttttagc aactcgcatg ctttggtgac tgcctctgat gcacctgcaa
     2401 tcacaatttg tcctggggca ttgaaattaa ccgcttccac aatctttcca gattgcgtac
     2461 tagcagatgc gcatacctca ataactatcg tatcttctaa acctaagatt gccgccatac
     2521 cgcctgtgcc aactgggacc gctgactgca ttgcctcagc acgaaaacgt actagcggaa
     2581 ctgcatcttt aaaactaata acgccagaag ctactaaagc ggagtactcg cctaaactat
     2641 gaccagccat aacgctgggt tttggtccgc cagcagccaa ccaagctcga taacaggcga
     2701 cccctgcggt aagcatcact ggctgggtat tggtggttaa tgccaaagcc tctgcgggac
     2761 cttcagcaat taatttgccg atattctcac caagagcatc ggatgcttct tgcaaagtgg
     2821 catgaacctc agtccgagca gcaagggaat ttagcatccc taccgattga gaaccctgtc
     2881 ctgggaaaac aaaagcaaat gtcatatcaa tctcatgaaa tagttttata tggagttaat
     2941 caattagtag cgcaagatta ctgcacccca agcaaagcca ccacctactc cctcaagcaa
     3001 tacgtgttgt ccgcgcttaa tgtgaccaga acgaatgcca ctatctaaag ccaatggaat
     3061 cgatgcagcc gaggtattgc catgctcagc aacggtcaca attaccttgt ccatcgacat
     3121 tcccattttg cgcgcagtgc cctccatgat cctaatattg gcttgatgtg gaaccaacca
     3181 atcaatttga tcagcggtca tattagcctt ttctaaaacc tcgttagcaa cctgctctaa
     3241 aaccttcacc gctaatttaa aaaccgcttg accatccatg gtcaaaaatg gtgagccttc
     3301 gataccacca cagattgctc gtccaggcac gcacaaaata ttgcgctgac taccgtctgc
     3361 atgcaaagca ctggccaaga tgccaggttg atccgatgcc tcaagtacta cagcgccagc
     3421 gccatctcca aacaatacac aggtagagcg atccgtaaaa tccaatatac gagagaacac
     3481 ttctgcgcca atgactaata tcttttgatg cgcaccagcc cgaataaatg catcagcaat
     3541 gctcaaggca tacgaaaacc ctgcacagac agcctgcaca tcaaaggctg cgcacccatg
     3601 cttggcgcct aatttatcct gtatcacgca agccgtacta ggaaacccac ccaaatgatc
     3661 aggagtggac gttgccaaaa taatgaggtc gatatcactg acagcgcatg cggctgactg
     3721 cagtgcagct tgcgctgctt tgactgctaa atcgctcgtt agctcatgat ttgcagcaaa
     3781 atgtctcgca gagatcccgc tccgggtttt aatccactcg tcactggtct ccaaaccttg
     3841 ttttgcaaga cgcgcaacga gatcttggtt gctaacgcgt tgctctggca aataactgcc
     3901 tgtgcctgca atgcgtgcat agcgaacggc gctcatacgg acctcatacc aaaggtttct
     3961 gcaatcttat caaccatccg gttcttagca gcgtcataag cccggtcgag ggcaacctta
     4021 aatgcaaagc gatccgccga tccatgactc ttgatcacac aaccacgtaa tcccaataaa
     4081 accgccccgt tatatcggcg gtggtctact cgcttacgca ccctccccaa gggtaagagt
     4141 gctgccaagc ccattaattt agttaacagt gaacgactaa attcttcgcg gatcatgccg
     4201 ctcatcatct ttgctaagcc ttcgctggct tttaaaacta catttcccac aaagccatca
     4261 cagacaacaa tatcagttgt tcccttaaaa atatcattgc cctctacatt gccatagaag
     4321 ttgagattac tttgccttaa caactctgct gtttgtttga caacatcatt gcctttgata
     4381 acctcttcac caatattcag taagccaatg gatgggcttg gattaccgtt cacgacacgt
     4441 aacatcacat cggccatttg ggcaaactgt agaagatgaa tcggctcaca atctgcattg
     4501 gcccctaaat ccaatactgt cgtgccctta cccagctcat ttgggatcgc cgttgcgatg
     4561 gcgggacgat caataccatc caaagttttg aggagatagc gagaaatcgc cattaaggcg
     4621 ccagtattcc ctgcagatat cacggcatct gctttacctt cttgaacttg tgtaatcgca
     4681 acccgcatag aagaatcttt tttgcgacgc aatgccacct caatggggtc atccatcaga
     4741 acaatttcgg tggcgccaat aatctcaaaa cgagtaagca tttctgcatt acattgttta
     4801 atggcttcat taagcaattt tggatcgcct actgcgatta ccatggcatc aggatgttct
     4861 tgcaaaaaat cgcagcaggc tgggatagtc acagagacac catgatctcc gcccatagca
     4921 tcgactgcaa gggtcacact cattgtttta tgtttgcaac gtggattgtg tctaagaaaa
     4981 aagcggcttt gagccgcttc aatccagcaa tatgaaatta gtcgtttttg gtcttaacga
     5041 cttttcgacc acggtagtag ccatttggag aaacgtgatg acgcaaatgc gcctcaccag
     5101 tagtggactc aaccgcaatt gcaggggcgg tcaaaaagtc atgggcgcgg tgcatgccac
     5161 gtttcgaagg ggatttttta ttctgttgga cggccataat gaactcctaa gcaagcaaga
     5221 attctagcat agaaacaggt aaaaacccgt attcggtgac caatttaata ctatttcttc
     5281 agacttttca gtaccttaaa gggattttgg ggttctgtac cccctccctc ttggctaatt
     5341 tggcagtcct caatggagtg ttttggggca ttggggatag ctagcaagat ctcatcctca
     5401 atagcctcca agaggtcaaa gctgtgactg gcaaccattg cctcttcggc gtcattgtcc
     5461 atggggaaat cgtcagcctc ttcttcagtt ttcacaaaaa caaagtgttt tttctgccca
     5521 aactcaaata ccaagggctg caaacaacgc tgacaggaca gtgttaattg ccccttaagg
     5581 ttaatttcca atactggcat tccatctcga tgatggccgc gtagttcgca ataaaaacca
     5641 tcgaggggat caagatgtgc agcctcattt cgcaatctgc cgaaatctgc cagattcagg
     5701 aaaccctctc caacataaga aactggggct aatatgtcta tcttcccaag ggattttggg
     5761 gtgattgcca gctgaacgat tggataagca gaatgagaac tcataggaga agtctaaaac
     5821 gagtcttgaa actaagccac aatattgcaa tattgaattc cccaatgtaa ttttcttatg
     5881 gcaaatacca aacaacttgt tctggcttct agctctattt atcgtcgcca actgctagag
     5941 cgcttgggtt tgccatttac tattgcgtct cccgatgttg atgagacccc tctagagcat
     6001 gagttaccag agacaatgac acggcgactc gcccaattaa aagcaagtgc tcttagcaaa
     6061 cagttccccg aggcatggat tattggctcc gatcaaagcg cagaccttca tggccaaatc
     6121 attggcaagc ctggcaatca tgagcgagcg cttgcccaac taaaacagat gcaagggcaa
     6181 actgtcgtct ttcataccgc cttatgtctt cttaagggaa aagaatataa aacgcttagc
     6241 gttcccacgc gtgtgcgttt tcgtaatcta cctgatgaaa gcctagagaa ttatttacgg
     6301 attgagaaac cttatgattg tgcagggagc gccaagtcgg aaggaatggg aatcattctt
     6361 cttgagcaaa tagaaagtga tgatccaact gctttgattg gtctacccct aattgcactc
     6421 tctggccttt tacgcgaagc gggttttgta attcctcatg tctaagtctt caaatacaag
     6481 cgtcttagga tgtttatatc tagtgcccaa taccctcggt gaagaagatc gagtgacaca
     6541 attatcgcgg gtcattccac cggatgctct ttgccaaatg gcctctctca aaaattggat
     6601 tgttgaggat gcaaagaccg caagagccct actcaacgcc gtcaaccaaa ttactccttt
     6661 gagtcaccct ctccaaagca tgcaaatgaa cgaatggcgc ggacctcaga gtaaaaacaa
     6721 gactggtctt aaaccccatg aactactaaa acccttaata cagggtgagg atgtgggcct
     6781 gatgtcagaa gcgggggttc caggtgtcgc tgaccctggt gcagagatca ttaatatggc
     6841 gcatcatttg ggggcacggg ttaaaccttt ggtcgggcca agctcgatct tgttgggttt
     6901 aatggcgagc ggtttaaatg gtcaatgctt tagttttcat ggctaccttc cggttgatgt
     6961 tgtcgagcgc tccaaaaaga tcaaacagct tgaacatcgc tccaaacaaa atcatgaaac
     7021 tcagctatgg attgaaacac cctaccgtaa tacggcgatg ttagaaactt gcctagccac
     7081 tctcctacct cagactcaac tatgtctagc catcgactta agtcttgaaa ccgaatggat
     7141 ttggacggcc cctatcaacg aatggcaaaa acgatttacc aatcaatcca gcatgaatga
     7201 tgttttaaaa aatcgtcctg ctattttttt aatgctggct tagcgcatct ctgcgccacg
     7261 catcagtgct ttgctaattg cggcgcctgt ttcggcacca aaacgcttag cgactcgttc
     7321 agcaaaattc tctttgagtg tgtagtcaac caaatcaggc gctttcaaaa tatcgcgcgc
     7381 cacagagtca atcgtgccat acccatcgac cagtcccaat tgcaaagctt gttcgccatt
     7441 ccatacgcgc ccagtaaaga tgtcaggatc attttttaag cgttcgccac gaccctgctt
     7501 taccactgta ataaattgct gatggatgtc ctcaagcatt tttttaacca tctcaacttg
     7561 tttggggttt tctttggaga aaggatccaa catacctttg tctgaccccg ctgtgattaa
     7621 gcgcctagag atgcccattt tttccatgag tcctgtaaaa ccaaaaccgc ccatgaccac
     7681 gccaatagag cctacgagac ttgccttatc caccaatatc ttgtcaccag caacggcgat
     7741 gtaataaccg cccgatgcac atacatcctc aacgactaca taaaagggtt tctcaggatg
     7801 caaggttcgc aaacgtctga tctcatcatt aatcatgccc gcctggacgg gtgaaccacc
     7861 agggctatta atgcgcaaaa cgacaccaac tgcctctttg ctttcaaagg ctgcggtaag
     7921 cgctgaattg atatccaagg catttgctat agagtttggg gaaatctcac cctcaatgct
     7981 cacgagggcg gtatgttttc cacttccacc actacccggc aactgaatat caaacacttg
     8041 ataaattaag cccaccaaca caaacaaact caatatcctt aacagagctt tccagcgcct
     8101 tgccttacga ttctctttta agttctcaag caagagcagt tcaagcgcgt ttcgctccca
     8161 ttgagagttg gatttttctg attcagggct ttgctttgta tccatagtga actctattta
     8221 cttatctcaa taggggcggg atgctgaatg tctaaattac tgtacaacca agcagctaac
     8281 tgttccacat tatcaaggca ggcgagagat tttgcagacc tcaaggcatc ggctggatgt
     8341 gcgccgtagc ttactgctat tccatcaacc cctgcggact cagccatctc aaggtcatgg
     8401 gtggtatcgc caatcattaa cattttgcgc accggtactt gtaggctatc cgataggtct
     8461 agcaacatgc cgggatgtgg cttagaaaag gattcgtcgg cagtgcgagt gtcatgaaag
     8521 atgtgcttga gttcatgaaa gtccaaggag cgatttaacc ctcgccttgg cttaccggta
     8581 gccacgccca acaaaaatcc tttgtttttt aagtcctcta ataactctct gatacctaca
     8641 aataagtgaa gttcatgatc tttagagaga tagtgataac gaaaccgatc gactaattca
     8701 ggaaagcgcg cgggatggac cgaaggagcc actcgacgta atgaatcttg cacacccaaa
     8761 ccaatcacat agcttgcgat ggaatcatcc ggaaccggaa aatcaagatc acggcaggcc
     8821 tgctggatgc aatgcacaat cgtgggcgtc gaatccataa tggttccgtc ccaatcccag
     8881 acaatcaaat cgtagcgtcg ctcttcgtat ttcactaaag ttctttctta tgtttgcatg
     8941 ggtagagtct tgcactccca ccgagttaac cattctctga atgcctttgg tacatctgcc
     9001 tcgatccgca ttttttcctg tgtcttgggg tgcgcaaata ctgcgaggtg agcatgcaag
     9061 aacaaatgct ttaccttaag acgtttatct tcatcatcaa taccgtattt atcatccccc
     9121 aaaatgggat ggccaatctt ttgcaagtgc acccgaatct gatgagtacg acccgttttc
     9181 aattgcgctt cagcaagagt aatgctgcct agatcacttt ttagaatctc cgtgacccga
     9241 agattggtat gactaacctg accctttgga tcaacccgaa ccctgcgttc tccattttcc
     9301 aaaagatact ttagtagggg aaacttaagt tgcgcagtac cagccccttg agaaatctcg
     9361 ccatgagcca ggagcctata acgcttatcc gttaaaccct cacggatctg tcgatgcatc
     9421 tctacaagag cgctacgttt ttttgcaaag atcaaaatgc cagaggtatc acggtctaga
     9481 cgatgaacca attctaagaa cttactctct ggtcttgcct ttcggagtaa ctcaatcgcc
     9541 cccaaactca cacccgatcc accatgaaca gccaatccgg agggtttatc gatgatgagc
     9601 aaataatcat cttcaaacag ggtgggtatc tttgcagcca gctcagttgc cttcgtctga
     9661 gaaatcccag caccagcttt tggggtttcg gctagccgag tgggcggaag gcgaacaata
     9721 tcgccctcga caagcctgga tgtcggtagg gccctttttt tattaatgcg gacctcaccg
     9781 gagcgaataa ttcgataaac atggcttttg ggcacaccct tagcccagcg gagtaagtaa
     9841 ttatcaagcc tctggccagc agaatcttgg ccaatggtct caaaaacagc ctgtaatgca
     9901 ggtgttttac tagatttctt ggcagaaacg ctcttttttg gggcgggatt aatggttttc
     9961 ctcatgtgat aggccccatt gtcattcgtt ttgtgaccta taatcaagga tctaggtaat
    10021 ttattaccag attgatttga attccggagc ttggagtcgc ccattaatag actcccgggg
    10081 ttgcccctct cccgttttaa tgaggcgcag ggttggcgtg atgtatgccg cgacccacga
    10141 agcagatgac cttttttagg cgagcgccta cattgagatc ttaaggagat ctctgtggcg
    10201 agcgttggtg tgagattcaa actatggaat tcttagtttg atcgttattt cctgtagagg
    10261 aatgttttag actctctcac ctcttaatgt ctgaggattt attccttaga acaatcttag
    10321 tcatcctctt cgcgtcgcac aaaagcgcct cccatgaagg agagtgttat gaaacgcatg
    10381 ttatttaatg caacgcaaca ggaagagttg cgagtcgcaa ttgttgatgg tcagaaatta
    10441 atcgacatcg atattgaatc cgccggacgc gagcagcgca aaggcaatat ttacaaaggt
    10501 gtcattaccc gcattgagcc ctctctcgag gcctgctttg tgaactatgg tgaagagcgc
    10561 catggcttct tgccatttaa agaggttgcg cgcacctact ttaaggaagg tattgatgtc
    10621 cgcaatgcgt cgatcaaaga tgccttgcgc gaaggtcaag aaattattgt tcaggtagaa
    10681 aaggaagagc ggggccaaaa aggtgctgca ctgacctctt ttgtctccct agctggtcgc
    10741 tatctcgttc taatgcccaa taatccccga ggaggcggag tatcccgtcg tatcgagggg
    10801 gaggatcgtc aagagctgcg tgaggcgatg gcacagttag aggtgcctga tggcatgagc
    10861 attattgcca gaactgcagg tattggtcgc gatgccgttg aattgcaatg ggatctgaac
    10921 tatctcatgc agttatggaa agccattgat gaagcagtca agggaaactc agcgccatta
    10981 ttgatttatc ttgaatcaag cttggtaatt cgtgcaatcc gcgactattt ccaaccggat
    11041 atcggtgaga tcctgattga taccgatgat atctatgagc aagctcaggc atttatgtcg
    11101 gtcgtgatgc cagataactt accgcgagtt aagcgctatc aagacgatgt gcccctgttt
    11161 tctcgattcc agattgagca tcagatcgag accgcctact ccagaaccgt ccctctacca
    11221 tctggtggag caattgttat cgatcacact gaagccttgg tatcggttga cgtcaactcg
    11281 gcgcgtgcaa cacgtggatc tgatattgaa gaaactgcta ctcgtaccaa ccttgaggct
    11341 gccgatgaga tcgctagaca aatgcgtctt cgtgacctag gcggtctgat cgtgattgat
    11401 ttcattgata tggaatcagc gaagagtcaa cgagatgttg aaaatcgctt acgcgacgcc
    11461 cttcgtcatg atcgtgcacg ggttcaaacc ggcaagatct ccaagtttgg cttgatggaa
    11521 ttatcacgtc agcgtttgcg accagcccta tcggaagcaa gtcatgtcac atgcccacgg
    11581 tgtaatggca caggccatat tcgtgataca gaatcctctg cccttcaggt attgcgcatt
    11641 attcaagaag aggcgatgaa agagaacaca gctgcaattc actgtcaggt tccagtcgat
    11701 gtcgttgcct tcctcttaaa tgagaaacgt gctgaggtta tcaagattga gtctcgcttt
    11761 aaggttcatg tcttaatgat tccaaataag catcttgaga cgccccatta caaattagag
    11821 cgcttacgtc acgatgaccc ccgcctagat gaacaaaagc ttagctatgt aatggtggaa
    11881 gagtctacgc gtgagcttga gaccgacact gtgattggtc gcaagaatga agaagtgcgc
    11941 acacgtcctg aggcggccgt caagggcatt actcccagcc agccagcgcc catggcaagt
    12001 gctcgccccg ctcgcgaaag cgcctctgct aaaacaggtg aggcatcctc tggtggaatc
    12061 ttcggattct ttaaaaagat attctctcct gctacccccg ccaaacccga agaggtcaaa
    12121 cctaatgctc gtccagaagg tcggggacgt aacggcgatc gcaataatcg taatcgcggt
    12181 cgcaatcgtc aagacagaac tgcgcgtagc gataatacgg atcgtccaga aaaagctgca
    12241 gatactggtg agccaaacca gaatcgccca cgtcaggcag gtcggggacg taaccgcggt
    12301 gagcgcaatc aacagcaaga gcgtaacgat caagcatccc cccaacaaac taagggtcca
    12361 gcgcctgact taagcgattc agaagtgagt aacgaggatc agcgtcgtgg tcgtaatcgt
    12421 cgtggtcgcg gtcgtaaccg cggtgagcga tctgagcgca atgacagtcc cgcccaagaa
    12481 ttaaatagcg gtattccgat gggtctaggt ggtcgttctg cctcaatgcc gctatcggat
    12541 ctgactcgca gctttagaga gggttctgca ccgcgtcgtt caggatctaa tcgcggcccc
    12601 agaccagaac aaagagtaca aagtgagagc gtccctactc caacatctag cggccagagc
    12661 atttcggaga ctagagttac agcaccgatt attgttcccg aactacccaa agttgccttc
    12721 acgccacttg ctgaagaacc gctaaaaaat gttattcaga ctgctggaat ggtttgggtt
    12781 ggtaccgata gcaacaaact cgctgaagta caaaaccaga ttcagtctga gagccctgcg
    12841 cctagagcac aacgtgagcc aaagccacct accaatctcc ctgcagggcc tatggttctt
    12901 gtggaaacgg gtggccaaga aaaaaccatt gataaaaatg gttagagctt ccgtacgcct
    12961 aagatagact tttgaagcca taatgcatca tggttgaacg cgtaatcccc attcgcaaag
    13021 agactcgaga gggtcctttg ccattgattc ctgagactct ttctgaggct catggtcgaa
    13081 ccttggatct acgacataga cctttgcgag acttacgcat atccgtgacc gatcggtgca
    13141 actttcgttg tacctattgc atgcctaaag aggtttttga tcaaaactat ccgtacctag
    13201 ctcaacaaga attgttgagc tttgaagaaa tttatcgctt agctcatatt tttgccacac
    13261 tgggtgttga gaaaattcgc ttgactggtg gcgagcctct tctgcgtaaa gatattgann
    13321 gatctaacac tcacaacaaa tggcagtctt ttgcaaaaaa agtcggcgca gttaaagacc
    13381 gctggcttac agcgtattac cgtgagtctt gatgcaatcg acgatgccat tttcaaaaaa
    13441 atgaatgatg ttgactatcc cgtttccgat gtcttaaatg gaattgaagc ggctaagaac
    13501 gctggtttta ccggaattaa agtcaatatg gttgttaaaa aaggtacgaa tgagcaagag
    13561 attttgccaa tggcaaaaca ctttcgtaat agcggggtta ccttacgatt tatcgagttt
    13621 atggatgttg gtagttcaaa tggttggaac ttggctgagg tgcttccctc ggcagatgtc
    13681 attgcaatga ttcatgagca ttttccactt gagcaagttg atcccaatta ctctggggaa
    13741 gtagctcagc gttggcgcta tcaggatggc tctggtgaaa ttggtgtgat ctcgagcgtg
    13801 acccaaacct tttgccacga atgtactcgc gcacgtctat ctaccgatgg ccaactattt
    13861 ttatgtttat ttgcgacgca agggtttgac ttcaaaacac ttctgcgttc tggaaaaagt
    13921 gacctagaaa ttgcgaatgc agtgatgcat acctggtcct ccagagagga tcgctactct
    13981 gaactaagac acctcaatac ccaagatccg aaacgagctg atcgtaaagt tgaaatgtct
    14041 tatatcggcg gctaattcaa tgggatcaat tgcggtatcc gatatcactg gtcttgtctt
    14101 agcaggtggt cgcgctcaac gaatgggtgg tattgataaa ggcttgattc cgtttaaagg
    14161 attgccttta atcgagtctg ctctcgaaag actcaaaccc caagtgaata cgatactgat
    14221 caatgccaat cgcaaccatt caatctactc atcgtatggt tatcctgttt tagcagatga
    14281 ggacgatagt ttttcaggcc ccctagctgg gtttgtggca ggactcaagg cttgcaaaac
    14341 cccctattta atgaccgtac cctgtgattc cccactcttc cctcttgatc ttgccaagca
    14401 actggcgatt actcttgagg cagagcagtc aaagattgga tatgcatcta gcaaagacat
    14461 ggccggtaag gtatgggcac aaccggtctt ttgtttgatg agtagagatc tactggattc
    14521 cttaacaagc ttcttagcga gtggtgaacg aaaaatagan nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gctggaatca
    14641 tttacacatt cgcattgatg aataaaccag taaacgccaa tcaagagttt cagtctgatt
    14701 ttttatcagt taccgaggca cggaccgcca tctccgctgt catccattcg attcaagcgc
    14761 aagctagact tcatgaggcc atagaaagcc tccctctaaa agagtgttta ggtcgcattt
    14821 tggcaagtga tgtgttatcc cctatcaatg ttcctgcaaa cgataactca gcgatggatg
    14881 ggtttgcatt caatggtaaa gagcttgatg cagcgcaaga ccctgtcaat ttacgcatcg
    14941 cgggtgcgat ttacgcaggt caaacccatt ccccgtctat tcaaatgggt gaatgcatta
    15001 aggtgatgac tggcgcaatg atgcccccga attgcgatac cgtaattcct caggagttta
    15061 cgaagtcaag cgaggaggga tcaatcacct ttgctaaaga tactgttcgt gcgggcgata
    15121 atcgtcgttt atgcggtgag gacttacaag cgggtaagcc tgcaatccag cgtgggcgga
    15181 tactaaaagc atccgatatt ggtttagcgg cctcattagg aatcgccaag cttgaggttt
    15241 atcaacggat taaagttgct attctctcgt cgggtgatga gctctgcagt attggtgtcc
    15301 cgttacagcc tggcaaaatt tacgatagta atcgttttag tcttcatgcc tgccttagta
    15361 agcttggctt tgaaatcatt gactgcggga tcgtccgcga tacccccgat gatcttcgtc
    15421 aagcatttat gcaagcagca cagaaagcgg acgtaattat ctcctcaggc ggagtatctg
    15481 ttggagaggc tgacttcacc aaacaaatta tggaagagtt ggggcaagta ggattttgga
    15541 aaattgcaat gcgtccaggc agaccaatgg cttttggtag cctaaagccg gtttccccaa
    15601 agacggctcc caccgtattt tttggtttac cagggaaccc cgtagcggtg atggttacct
    15661 tttaccaatt tgtgcgtaat gcgctattac agttaaatgg cgcccttaac cctgaaatcc
    15721 cgctcgtatc aatccaatct gccgaggcta ttcggaaaaa gtcgggtcgt acagagttcc
    15781 aacgcgggat tttgtttcgg gatccagaag gtaggttgtt agtcaaatcg actggtagtc
    15841 agggtgcggg cattctgcgc tcgatgagcg aagcagattg cttcatgatt cttcatcacc
    15901 atcaaggcaa tattgccccc ggagactggg tggatgtaga gatttttgat ggactgcttt
    15961 aagattgcag gatcatgaaa ttcactaaaa aagaaattgt cttcttagac ccaatgcata
    16021 ccgccaaaac tcttgttttg gtctacctat gcttctccgt acccatcgtt ttattagccc
    16081 tttttgtagc ctttattcga gatggctcga tgcccgggtt tactgtgatc tcagcactta
    16141 ttttgaacgc tgttctgggc tttggacttc tttggatcgc atgtaaagtc tacaactggg
    16201 ttgcccaaaa atttgggggt attgagttag cccttaaaga actatctgaa gagactgacg
    16261 atatccagta aggtattttt gtgtacccta tttcttccag agctcggtat taattaaact
    16321 gggtggcttc tcacccgcta atgctgctcg caaattacga actgctaagt taatcattgc
    16381 gcgccgagtc ttctcagtgg cactcgcaat atgtggggct agtaccacat tgcttaactt
    16441 caagagctca ggatgaacct tcggctctcc ctcgtataca tctaagccag cagcaaagat
    16501 tttttttgcc ttcagggctt ctgccagagc ggcatcatcc acaataccgc cgcgcgcaat
    16561 attaacaagc gttgctgtgg atttcatcaa cgcaatttct tttgcgccaa tcagatgatg
    16621 gctttctgca gaatagggga caactaaaac cacatggtct gcttcgcgta ataaggtctc
    16681 tttatcaacg taacgagctc cacaggcttt ctcatcggac tcacttaggc gcttacgatt
    16741 gtgatagatc acattcatac caaaaccaag tccacgcttc gcaattcctt gtccaattct
    16801 gcccatacca ataatgccaa gggtggtgtg atgcaaatcc atccccaatg ggttgtatac
    16861 gatggacatc ttgtcccact ggccattacg aatccaatgc tcagactccg tgatacgtct
    16921 agcagcagcc atcatcaacg caaagccaaa gtctgccgta ctatccgtta aaacatctgg
    16981 ggtattcgtt gccatcaccc cagctgtggt cattgctggc acatcaaaat tgttataacc
    17041 caccgagata ttggccacaa tttttagatt ctttgcatcc gccagggcat tttgatcgat
    17101 tcgctcactt ccgaatacca gtgcgcccgc aacttgtgca agctctttgc gtaagttatc
    17161 tggggagaag acctcatcgg cctgattgga cttcacctca aagctctcct ctaagcttgc
    17221 taaggcatct ggaaaaatag ctctggcgac aagaattcgt ggtttagtgg acataggatt
    17281 caatagaaaa tgaggtaaaa ggctcggaag caaaacttta caccatttgg gctaaaatca
    17341 attcagaggc aataatctgt tgtattgctt ggtttatatc cttaaacaat cgtcgtagag
    17401 ccaaatcatg acttacgttg ttactgaatc ttgtatccgt tgtaaataca ccgattgcgt
    17461 ggacgtctgt ccagttgact gttttcgtga ggggcccaac ttcctaacaa tcgatcctaa
    17521 tgaatgcatc gattgtgcag tttgcgtccc agagtgtcct gtcaatgcaa tttatgcaga
    17581 ggacgatgtg cctggtgacc agcaacaatt tatcaaaatg aatttggagc ttgccaaaca
    17641 gtggccatcc atcaccaaat caaaggctcc actgccagat gcagatgatt ggaaggatgt
    17701 aaagagcaag cttgagcatc tcgaaaaata atcctcgcca acgctccatt cactgagcct
    17761 tttctatgcg ccaatttcat gagttaatgc gccatgtcct tgatcatggg gcaaagaaat
    17821 cagatcgtac tggtacgggc actttatcgg tctttggtca tcaaatgcgc tttgatttat
    17881 cagaaggttt tcctctggtc accacgaaga aacttcacct caaatccatt gtgtatgaac
    17941 tcctttggtt tctaagtggt aatactaata atcaatggct caaggaacgc ggagtctcta
    18001 tctgggatga atgggcggcg cctgatggtg atcttggccc ggtctatggc taccaatggc
    18061 gctcttggcc agcaccgaat gggcaacata ttgaccagat caaggagatt attcagacca
    18121 ttaaaaccaa tcccgactca cgacgcatta ttgtgtcggc ctggaacgtg gcagatatcc
    18181 ccaagatggc gctagcgccc tgccatgcat tcttccagtt ttatgtggcc gatggtaagt
    18241 tatcgtgtca actctatcag cgcagcgcag atatattttt aggtgtacct tttaatattg
    18301 ctagctatgc attgctgacc catatggtgg cgcagcaatg cagtcttgat gtcggtgagt
    18361 tcatttggac tggtggtgat tgccatctgt acagcaatca cctcgagcag gttgagttac
    18421 agctatctcg aaagccatac tctcttcccc aattaaaaat ccatcgtaag cccgatagta
    18481 tttttgacta ccaatttgag gactttgaaa ttgtgggcta tgaatcccac ccccacatca
    18541 aagctccagt tgcagtttga gctaattagc ccatgagtac acctgctatt tcaatgatcg
    18601 tggcgcgctc tcgtaatcat gtcattggca aagataatca aatgccatgg aaaatatcgg
    18661 ctgatttaca gttcttcaaa aaagtaacca tgggataccc catcattatg ggtcgcaaaa
    18721 cctgggaatc aattggtagg cctttgccgg gtcggcgcaa tgtagtggtg agtcgtaaca
    18781 ccaattactc agcaatcggt gctgaattgg ttggttcctt agatcaggct ttagagttgt
    18841 taaaagaatt taagcgtgtc tttgttattg gtggtcagca attatttacc caagccttcc
    18901 ctcaagccga tgagctcttc attactgaga ttgaaattca ggtcgatggt gatacgtatt
    18961 ttgaaattcc agatcccaat gactggcaag aggcagaacg catcttagaa agtgagggcg
    19021 atattcagtt tgcctatgtg actcttcgac gaaaggcagt ctaattactt tccaccaatc
    19081 gtcattgagc ccagcaaaat tgatccagtc tctttagttc cccgaataat cgtgtcatcg
    19141 cccaccgcct gaatatccat caacatttct tttaagttac cagcgatagt gatttcttca
    19201 acaggatgtt ggatgatgcc gttttcgacc caataaccaa atgcccctcg cgagtaatct
    19261 cccgtcacat agttgacccc ttggcccatc agctctgtta ccaacaggcc ggttcccatt
    19321 tcttttaaca aacctggcaa gcccccagat ggggtatgca tactatgcaa cttcagatga
    19381 tgtgcgcctc cagcattacc ggtagtttgc atgcccaact ttctggctga gtaggtagac
    19441 aagaagtagc cttgcaattc tcctttggat atcaccgacc gagctttagt gcggacaccc
    19501 tcttcatcaa acggtgcgct tccactcatt cgctttaggt ggggcaactc aaacaagtcg
    19561 atatgctttg gcattacttg cttccccagg ctatcgagca aaaaactaga acgacgatac
    19621 aaagcaccac ctgaggttgc ttggaccaaa gagccaatca gaccgactgc gatcggggct
    19681 tcaaagatca ctgggcaacg ccgtgttgtt aacgattttg catttagctt tgccaatgct
    19741 cgttgggcag cataccgacc aatcaaagag gggtttgcta actcactcgc aatgcgagaa
    19801 gtgctgtacc aatcatcgcg ttgcatcgca ctcgaatttt tagaggaact ggcaatgggg
    19861 gcgcaggata tgaaatgcct tgaatatgga tatccaccca taaagccatt actggttccc
    19921 agcataaagt gcgcctgatg ggcagatacc gatgccccat cactgttcag gattgcttta
    19981 tcgactgcaa atgctgcctt ttctgcttta cgagcgatct gaatcgcctc tttagtatct
    20041 agatcccagg gatgaaataa atcaagatcc tgagggttct gctctaaaag atctggctcg
    20101 gccaaaccag cgcaatcatc ttctgcagtg tgttgggcaa tatgaaaagc cgcctctacc
    20161 gttaaacgta atgagtctgg agaaaaatcg ctggtactgg cattgcctcg gcgctgacct
    20221 agatagatgc ttacgcccac ctgcttatca acactttgct caatggtttc tacctcaccc
    20281 ttgcggactg ttacagagag accttgaccc tcggagattt ctgcggcggc gtctgaagcc
    20341 ccaacgcgct tggcttctgc caaaataaag tcaataatga tcctaaattg atccggagaa
    20401 tatgcaaaca ttcctcaatg atagctagaa tatgtccatg aagcatcaaa acccttttca
    20461 tcgcaatagt cctaatgagg taaagattgg tttggtatcg atttctgatc gtgccagcca
    20521 aggggtatat gtcgatgaag gcatcccttc cttaaaaaat tggcttggca aggtgatcac
    20581 cactccagtc gtgttccatg agcgccttat tccggatgag gccgagcaca tttcatcctc
    20641 attgatcgag ttggttgatg agttggggtg tgatttggta ttgactactg gtggcacagg
    20701 cccgtcgcga cgagatgtga caccggaagc cactctggat gtgggaactc gtgaaatgcc
    20761 aggatttggc gagcagatgc ggcaaattag ccttcatttt gtgcccaccg ccattctctc
    20821 tcgtcaagtt gctgtattac gagagattga gaatcatgct gcattgatta tgaatcttcc
    20881 tgggcaacca aagtcgattc aggagacttt agaaggcttg aaagatgctg atggcaaagt
    20941 gcttgcccac ggtatttttg ctgctgtacc ctattgcatc gatttaatcg gcgggccttg
    21001 tattgaaacg gatgaggcga ttgtgaaagc gttccgtcca aagaaaaaga agtagcgcta
    21061 acagtctttc attatctctt ctaaacatta gttttgagag ggaataaaga atcgctcgcg
    21121 atagtatttg agctcttcaa tcgattccat aatatcggct aaggcagtat gggcttgttg
    21181 cttattaaat cccttcacaa gctccggttg ccagcgttta catagctcct tcacagtcga
    21241 tacatcgaca ttccggtaat gaaaataact ctctagcttt ggcatatagc gagccataaa
    21301 ccgccggtct tggcagatcg agttaccgca catcggcgca gtattggctt tgacgtattg
    21361 ctttagaaaa gcgatacatt gctcttccac cgccccctca tctaaatcag atgctctaac
    21421 cttatccact aagcccgatt tggtgtgagt gccagtattc caagcgtcca tgctgccaag
    21481 aacctcatta gcttgatgga ccacccatac cggggcggta gcaatgatat ttaggtgggc
    21541 atcggtcaca atcatggcga tctctaatat acggtcagaa tcaggcttta atcccgacat
    21601 ctccatatct acccaaatca tgggttctgc gctattacca gaccccttat tttccgtact
    21661 catctttata atgactccat gacctttaca tatctattta ttctggcttt agtccttagc
    21721 gttggtttac gccattggct cgcaatgcgc caaattcgcc atgtcgccaa ccatcgcgct
    21781 agtgtaccta atgaattcgc ccatacgatc agtttagtgg atcatcaaaa ggcagctgac
    21841 tatacgattg ctaaattacg tcttggcctg attgaaaact tctttgctgc ttttgtatta
    21901 attgcattca ctctattggg cggtctagag cttcttaatc aatcgctact cgatcttttg
    21961 ggtccaggca ctttgcaaca gatggctttg ttgatatcta tatttttaat ctcgggcatt
    22021 ttggatttac cttttacctg gaaaaaacaa tttggtattg aggctgccta tggctttaac
    22081 cgaatgaccc ctgctctctt ttggctggac atgatcaaag gtgtcgcatt agcctcggcc
    22141 cttgggcttc ctcttctctg gttggtcttg gagttgatgt ctagtagtgg gccctactgg
    22201 tggctatgga cctggattgt ctggaccgct tttaatgctc ttcttctttg gctctttcct
    22261 acctttattg cacccctgtt taataagttc aaggccttag aagatggccc cctgaaagca
    22321 caaatcgagc gtctcttaga acgttgtgat tttgctagtc aaggtttata tgtgatggat
    22381 ggtagcaaac gaagtgcgca tggcaatgct tatttcacag gcattggtaa ggcaaagcgg
    22441 attgtgttct ttgacacttt gattgagaag cttgagcccc tagaagtaga ggctgttctt
    22501 gcccatgagc ttggtcactt taaacgtcaa catattcgta agcgtttact agtctcattt
    22561 gccctgagct ttgtcatgct ggctattttg ggctgggtga gctcacagcc gtggttctat
    22621 ctcggtcttg gtgtcagccc cgatctcaat ggctataacg gcggtctagc cttggctttg
    22681 tttatgttgg ttgcccctgt atttagtttc ttctttactc cattnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt aatttctgct ctagtaaaac
    22801 tctatcaaga taatgcttcg accctaaccc ccgatccgct ctataccgcg ttctatagct
    22861 cccatcctcc tgcgcccctg cggattgcgc atttacaacg atttgcatga gcggtgtttg
    22921 ctgatgggcc aatttcgtgc cctgctaagc gcctcctacg ggagacatta cttagcgcaa
    22981 actattcagg cggatggctc ccatgctggg cccttaatac aagttagcac accggctaag
    23041 cgccacgtcg gcgctgtcgg tgatatcttg attctggagg agacaggtcc aaatcaggca
    23101 cgaattatcg aaatcgagaa gcgcaagaac ttactttatc gatcagacgc atttaagagt
    23161 aaatcgattg cagcgaatgt tgaccagatt ctggtggtgc ttgcaactgc tccggctttc
    23221 tcacccgatc tcctaggacg cgctgtagtt gctgctgagc tagatcaaat tgaactgcgc
    23281 atcatcctga ataaatgcga tctgcaggat caattaacga ttgcgcgacg aggtcttgtt
    23341 ccatatcaaa acatgggcta tccagtccac gaggtctcgg ctaaatttga tccccaatca
    23401 cttgagggtc tccaaccttt cttgaaagga aaggtatcgg tattggttgg gcaatctggc
    23461 atgggtaagt cgactttact gaatagttgg gcaccaaatg cggccgctct cacacaagaa
    23521 tattccttaa aactggatag cggtaagcac accaccacag catgccgtta ctttgattta
    23581 cccgagtggt ctcgcaatca agcgcattta ggggggatga ttgactcacc cggttttcaa
    23641 gaatttggtc ttgcccatct ctctgaaagc caactacagc atgcctttag agagttcaag
    23701 cctttgctgg gtaaatgtcg cttccataac tgtaagcatg atagcgagcc tggatgcgtg
    23761 atccaggagg ctgttcttgc gggtgagatt gctgcagaac gtctagcgct ttttaaacag
    23821 ttgctatcgg actctaaaac cgcagatatt caactgcagg gttttagcca agccaaagag
    23881 cgatggtcaa catcgccaaa accaccatac aaacgataag ggctcgccac accaagccga
    23941 tagctgatct catggagtgt tcgctaggct ctttcccaat ctcatagacg ggcggctcac
    24001 ccgtctcagc tctagatagg gcctcatcac tggtcggctc tcgtaatggc tcacccagac
    24061 ggacacccaa ggcaccacta ccagatgcca gtaatactgc acttaaaggg tccgaccact
    24121 tgcttgttaa attacgccac gcatagaccg agtcctcaaa attaccaacg atggcaaaac
    24181 tcatggcact caagcgaaca ggcaaccaat ctaatatata aaaaaagtgt tttgctgctt
    24241 cggataaatt aaacccatat tgctcccaac gcaaagaggc cttatccgcc aagcgatata
    24301 gaacaacgcc cgcagggcca attggtacta agaaccagaa aaagacccca aaaacatggc
    24361 gatgggcgcc aataattgcc ctctcgagcg ctaccgcaat aatctcagtc tttgataaat
    24421 gggtaacatc aatatcatcc cccaaccagc tttgaagagc agcacgggct gccggcacat
    24481 catggttttg aatggcttca tggacctctg taaaggaatg actaaattgt ctaaacccaa
    24541 aaaagagata aacgatgatg atgttccaga cgaaggctaa taagggatga gccacaaacg
    24601 ctacgacata aatagcaaac acaaaaatag taggcaagcc aaagcccact aaacaagcta
    24661 accgagcacc cactggtgtt gccccatctg cagacttgcc accaaactcc ttggcaaccc
    24721 aatcaagcca tgcaaggctc gccctccgga tccaatggga tgaggtaact ggccgatatt
    24781 gttcggcaat tagagcgaag aggatagaaa aaaaggtcat gcttttaata gatgatatag
    24841 atttcgtaac attccagcgg tggcgcccca aatgaaccgc tccgcatagg gcattgcata
    24901 aaagcggcgt gagccctctg tactttgcca tacccggatc tcatgattag caggatccat
    24961 taagaaacgt aaagggacct caaatacatc tgcgacctcg aagggatccg gccgatactg
    25021 cgaaacgggt ttcaccaaag caaccactgg ggtaacctga tagcccgaga ccgtaagata
    25081 ttctggtaaa gagccaatca cctcgacatt ctgactggga agcccaattt cttcctcgct
    25141 ttcccgaatt gcagttgcaa caatagaagc atccccctca tccattcgtc ccccaggaaa
    25201 actaatctga cccgcgtgat catgcaaatg atcggtacgt tgggttaata aaacatgcag
    25261 tccatttgac tgcattacca ggggcattag aaccgccgcc ttcgttatcg cgccctgctc
    25321 ttgcttgcgg gcgatgatat tacttgccaa tacatggcgg ttttcatcgg taatctcggg
    25381 ggtccatgtg ggtgaagtct caaactgagc acgcagccca tcctcacaaa ataaagcttc
    25441 gggtactggc ttttggccaa tgcaacgacg atcaatcggc actaagcggg gatcgaatcc
    25501 ggggggcgca gccaagcttt ttttgaggga gtccagagta ttcgacatgg atccatttta
    25561 ggtgatttaa gaaaacaaaa aggcgaccga ggtcgcctta gtgcacataa gagcgagatt
    25621 aatctgcggc tgtctcggca actgtttctt taacacgcgc cttgagcttc tctttaatac
    25681 gagcagactt accagagcga tcgcgcaagt aatacaactt cgcacgacgt acatcaccac
    25741 gacgcttcac ttcgatgcta gcgattaatg gtgaataagt ttgaaaagta cgctccacac
    25801 cttcgccaga tgaaatcttg cgaacgatga aactagaatt caggccacgg ttgcgcttgg
    25861 caatcacaac gccctcaaaa gcctgagcac gtttacgact accttcaacc acgtttacac
    25921 tgactacaac ggtatcgccg ggtgcaaaac ttggaatcgt tttgttggcg gttagacgag
    25981 caatttcttc ttgctcgatt ttttggatca agttcattac aactccttag gcatcatgtt
    26041 ggcgttaatc ccgtgatcaa tcagcgggcc caatagagga tgcagttaaa acaactaagc
    26101 caaaccaata tttttattac tctgagagtt tctttaaaaa cagctcatcg tcttggttta
    26161 gcaacccatt tgcgcgcgca gcctcaatga ggtctggacg acgctttaac gtcagctcta
    26221 aagacctttg ccgacgccaa tccattattt tagcgtgatg tccgcctaaa agcacgtctg
    26281 gaacgcataa attttcataa atttcaggcc gggtatagtg cggatggtct aaaaggccat
    26341 taacaaaact atcttgtaag gctgaattct cgtcccccaa cacccccgga atcaagcgaa
    26401 tcatggcatc cataaccgcc atggcaggga tttcacctcc ggaaagtaca aaatcgccga
    26461 tggatagctc aaaatccaca tttcggtcaa taaaacgctg gtctactgcc tcatatcgtc
    26521 cacaaattag cgttaagtta ctgtatttaa gcatatctgt tgctaacttc tgcgaaaatg
    26581 tctttccttg agggcttaaa agacaaactg gaccggactc tatcccagat tgttgatggg
    26641 atttctgaat cgccgccaag gtctcctcaa gcggcttaat gagcatgacc ataccaggcc
    26701 ctcccccata agcacgatca tcgactgttt tacgaggatc ctcacaaaaa tctcttatat
    26761 tccatgtact tacagttgta agtccgtgct cacatgctcg cccagttacc ccactttttg
    26821 tcaaagcggt gaacatttca ggaaaaagag tgacaacatc aaaatgcata cgggccttca
    26881 aattacttat ttgctccaat tggcttgcca ggcaaccact atggttccaa cgcccccggc
    26941 atcgctagcc tgaagatcta ctgactttac aagatcgggc acaaagggga tcagctcctt
    27001 ccctacagtc atgactggat gggcgccata ttcagcgatc tcatcaacga aaccaagttc
    27061 agcgccctcc tcattcagca ccctgcaacc aatcagatca atccagtaat aggtaccttg
    27121 ctccgtcttg gggaagctag ttctaggaag ctttaaggac gagcccttta ataacaaagc
    27181 agcatcacga tcggtaacgc cctcaagagc catgaccaca aaaccgctgt gtaattttgc
    27241 ttggttgact gtgtaggtag catcgattga agcgcctcga gcgccatccc gttgcaaaca
    27301 gatttgctta cattggagaa gggctatagg gtcaggagag taggggcgca ccttaatgtg
    27361 ccccttgagg ccttgggcat caaatacctg cccaagctct atcaggtcag aaggaggctg
    27421 atgaatcaca acacctccat gaaagctcgt aagcctatga attaggctgc aggatggtcc
    27481 ttgattaaac gcttaacggt tggggagatc tgagctccaa cgccagtcca ataagttaaa
    27541 cggtcctgag caaggcgcat tgcttgctct ttctcagctg cttttggatt gaaataacct
    27601 aaacgctcaa tgtaattaga gtcgcgacga ttacgtttat cggtagcaac aatgctgtaa
    27661 aaagggcgct tttttgaacc gccgcgtgcg agtcgaatga cgaccatatt tattccttaa
    27721 aatcctagta atacgggtta attaactggt taaatacacc gactgtctgc aaaggcaatg
    27781 caataaggtg tagagaccgc agaaaacctc atattctaga cgaaaaccct tataaactct
    27841 actaacttac caaactatta ttaaagataa ctataaaagt acatataaat caataactta
    27901 taaaaaaata tgcttaagct tccaactcct tttggtaaac gaatccaacg atccatcctt
    27961 ttggtctcgg gagtctgctt tttgggggct tgcgccaata tcaccccccc gtgcgccgcc
    28021 aaaaatactc ccccggctgc cgagcttaaa ggaacgcact gggaactgtc tcgctggaat
    28081 ctaccgccca atgcgatggg tgaggttcgt cttcgttcat taccaaatga tcagggtcaa
    28141 aaaatccaat tacagttcag cggcaatcga atgagcgggt atagcgcctg caatcgcttt
    28201 acggcacaga tcgcagaaga tactcgggga tttcagatta atcaaattgc tacaacgcgc
    28261 atggcttgct ctacagcacg agaagaaatc gaacgcgatt tcttgtacct cttaaatgat
    28321 tacagaacga ttgcgcgcga tggcgatcgc ttactgatta ttgggcccaa tcgtgaggtc
    28381 ttaagctttt ctcaaatcaa tctttcctcc aaatgacgca atcatttcga tcnnnnnnnn
    28441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28501 nnnnnnnnnn nnatcgaggc aagttggtta acaaccattg catttggctt acgccctgat
    28561 gaaaaatggg atgcgcagtt caatactgaa gctaaggata atcaaaagac agagtctgga
    28621 tggccggtgg ttatttccgt gatcctgtcg ttaatccttg gagcgggagt aatgatgatc
    28681 ttcttagcaa ttggctttga gcagttcttc atgtatcaga ttgaagaagc cagaaaaatt
    28741 agtcagtaac ttttactaaa tcgcagctgt taactcgggt accaaagtaa ataaatcccc
    28801 cactaagcca taatcggcta caccaaagat cggtgcgtca ggatctttat tgatcgccac
    28861 aattattttt gagtccttca ttccagccaa gtgctggatt gctcctgaga taccaaccgc
    28921 aatatatagc tgtggtgcaa cgatttttcc agtttgacca acctgataat cattcggcac
    28981 ataacctgcg tctacggcag cgcgggaggc tccgagagca gcacccaatt tatcagccaa
    29041 aggctcaatt aattccttat atttctcggc agaacctaag ccgcgcccac cagacacaat
    29101 gattttggca gcggataact caggtcgatc cgactttgtc agttctcgcc caataaattg
    29161 agatgcttca tatggatcta caggtttaat cgactcaatc acagccgagc caccctccgg
    29221 ggcagcctca aaatttgttg tgcgtaccgt gattactttt ttagagtcac tcgattgcac
    29281 tgttgcaatc gcattacctg cataaatggg gcgctcaaat gtatcgcctg agaccaccct
    29341 cgtaatatct gaaagctgag caatatctaa tttggcagca acacgaggca tcacactttt
    29401 tccgtgagca gtggcgggtg ccaagaaata atcataatcc gaggccagag caatcatttg
    29461 ttcggcaaca gactctggta gttgatctat aaactgtgtt gcatcggcat gcaaaacttt
    29521 acggatacca gcaatacctg ctgcagcttg agcaatagcc ccagcattgc taccgattaa
    29581 caacagatca acctcagggc tgcaaagctt tgctgcactc acagcatgat gggtagccgc
    29641 ttttaaagaa tgatggtcat gttcgaccag aactaaagca gccatttata gaacctttgc
    29701 ttcgtttttt aatttatcga tcaggctagc cacatctggt actttgatgc ctgcggtgcg
    29761 ctttgggggc tcttccaccc gaatcgtttt caaacgaggc gcaatatcaa cccccaaatc
    29821 ctctggctta agaacatcta gctgcttttt cttggccttc atgatgtttg gcagagttac
    29881 ataccgaggc tcatttaagc gtaaatcagt tgtgatcacg gctggcaatg aaatcgatac
    29941 ggtctcaagg ccgccatcca cctcgcgagt tacctgagcc ttgccatctg caatgcttac
    30001 ttttgaggca aaagttgctt ggggaaggtt cagtaaagcc gcaagcattt gaccagtctg
    30061 attactatca tcatcaatcg cttgttttcc taaaataatc agttgtactt gctctttttg
    30121 gtaaatcgct tgcagtaatt ttgcaactgc tagcggctgg agctcggtat cggtttctaa
    30181 caaaatagcg cggtcagcgc caatcgcgag agcggtacgc aaggtctctt gacactgttg
    30241 agggccggcc gagaaaacaa taacctcact gacgatgcct gcctctttaa gtcgaaccgc
    30301 ctcttcaacg gcgatctcat caaacgggtt catgctcatc tttacattcg caatatcaac
    30361 cccggattga tctgacttca cacgcacttt gacgttgtaa tcaacaactc gcttaacggc
    30421 taccaagacc ttcatttgct acctttatca accaaataag ttcttatttt atcgctattt
    30481 agagctcagc caaggtcttc aaatgagcag caacactcct acccaatgcg gataggttat
    30541 agccaccctc gaggcagcta acaatttttc catcgcaaga ctgattggca acattcataa
    30601 gctgctgtgt aatccacaca taatcatcct ctactaagcc catttgcccc aaatcatcct
    30661 ctcggtgggc atcaaagcct gcggagatca tgatcaattg aggcttgaac tcttgcaggc
    30721 gcggaatcca tatcttacta atcatctctc gaacaagctt tccgcttgta ctggctggca
    30781 aggggatgtt caccatatta tccccaccct ccaaaccact gtagggatag aacgggtgct
    30841 gaaagaagct acacatgagc acatggggat catcgataaa tgctgcttcg gtaccgttgc
    30901 catgatgtac atcaaaatca atcacggcaa ctcgatctag gtcatactgc tcaatcgcgt
    30961 atcgagttgc aatggcaatg gtattaaaaa cacaaaagcc cattgaacga tggggctctg
    31021 catgatgccc tggggggcga actgcacaaa aggcgttatt aacctcgccc ttcatcactg
    31081 cgtctaccgc tgcaatagca gctccagcag cacgcaaaga gacattccag gttgagctgt
    31141 tcatgatcgt atcaccatcg atcatggcgt agccactcgt tggagcctta ctttttatga
    31201 atgccaagtg atcctcgcta tgcacacggg taatatcagc ctcagttgcc agaggtgggt
    31261 caattcgttt taaatggcca tctaagcgac tctgaattaa ttgatcttca atagcttgga
    31321 tacgctcggg gcactcagga tgatggcgcc ccatctcgtg tttcataaaa tcgggatgac
    31381 tgatataggc tgtcgtcatt tacttgtctc tcttatattt tgtatacaaa ttactacttt
    31441 gctcactaaa ctagcatctc tatcaaaact acttctatct tagcgccaac ttgagaacac
    31501 tatcctacct atcgatttta gggctgagcc ttattttggg ggcctgcgcc acccccatcc
    31561 ccactccaat tacccaagaa ggttcggcct ctaccaaatt agaagaggta tcagaacctc
    31621 ctattgatcc aaaaacagag gcaaactacc aagagcagtt acgggcatta atcgccgagg
    31681 tctccaaaag tcagtcaatt cctctagaaa gccttgaact gggctttcgg gatagcaaaa
    31741 acttaagcca gattaagaaa ctcgttgccc ctcctccggt cacttttcag aagaattggc
    31801 gggtttatcg cagtcgtttt gtggaacccg ttcggattaa ggcaggcctc acctttattg
    31861 aacaaaaccg ttcagcctta gtggcagcag agcaagaaag tggggtgccc tatcaggtca
    31921 ttgcagccat tattggtgtt gaaacaatct atggccgcca aatgggtaac tttagagtga
    31981 aagatgtcct aacgagcctt tcctttgact acccagaggc tccaaaccga cctgctcgtc
    32041 aggccttatt tagggatcaa ttaaaagatt tgattgccct atgttggcga gatgcgaatc
    32101 gcaaacaaga aggctttaac cgctgtctta atcaaagtag ctcctacgcc ggtgccattg
    32161 gccttccaca atttatgccg ggcagtatct tacaatttgc taaggatggc gataacaacg
    32221 gaattattga tttgcgtaaa agtaatagag atgcaatctt tagcgtagcc aactttctta
    32281 aagtacatgg ttggcaggct ggtatgccaa tctactttcc taccataatc aatgagcaga
    32341 cgcggcccag gattatggag ctggcggatg gtcaacccat tgctaagtta acggtcaacc
    32401 agttaataga gttaggcatc ctagacccta agcaaggcga tcttcttcaa ggtggggttg
    32461 aatccaacag taaggctctt attgttgatc ttccatcccc tagtaacgga ctggatcctg
    32521 aagtggaata tgtcgtgggt cttgc
//
LOCUS       DDPQ01000055            8334 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11950,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000055 DDPQ01000000
VERSION     DDPQ01000055.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11950"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3148..3184
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4665..4724
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7074..7171
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2255 a         1808 c         1861 g         2213 t
ORIGIN      
        1 gaagtcggtt acgtcgtcat tacctttgta gctggtttgg tatcgtttgt tatagctctc
       61 gcgctgatat tggtggaggt tgtatttgcg tatcgctcga caagctggga tttgcctacg
      121 attgataaat ccaataaaac ccgtatgtat taaaaataca aaaaaattta ttaatcatta
      181 aaacaacgta ttttcagaag agagagatca gtatgtcctt taatcagaac aaaatcctta
      241 tcactggcgg cgcaggtttt ttgggttcgc acttaaccga gaaattattg tcacaaggca
      301 atgatgtttt ggttgttgat aattttttta caggaacaaa acagaatcta gcgcatcttt
      361 taggcaatac caatttagaa gtcatacgtc acgatgtgac cttccccctg tacgtagaag
      421 taaatcaaat ttataaccta gcctgccctg catctccagt tcattatcaa tttgatcctg
      481 tacaaaccac caaaacgagt gtgcatggag cgattaatat gctgggcctt gccaaaagaa
      541 ctagagcccg gattttgcaa gcctcaacta gtgaggtgta tggggatcca gaggtgcacc
      601 ctcagcccga aagctactgg ggtaaggtca atccaattgg tattcgctcc tgctatgacg
      661 agggtaagcg ttgtgctgaa accctgtttt tcgattatca ccggcagcat caaactgata
      721 taaaggtagt acgtattttt aatacctacg ggccccgcat gcaccccaat gatgggcgcg
      781 tagtgagtaa tttcattgtg caggcccttc aaggtaagga cattactatt tatggtgatg
      841 gttcacaaac acgaagcttt tgctacgtag atgatttgat agatgccatg atcaaaatga
      901 tgaactcaga aaaaggattt actggcccag tcaatattgg taacccgggt gagttcacga
      961 tgttgcaatt ggcagaaatg gttcttaagt tatcgaatag taaatccaaa attatttatc
     1021 aacccctgcc atcagatgac cccaagcaac gccaacccaa tattgattta gccaaaacta
     1081 agcttggttg gcagcccaag gttaatcttg aagatggttt aaaagagacc attgcttact
     1141 tcaaaagaat gatctaacgg gctttaatgg aaaaaaccaa agccatcgat gggcggattc
     1201 gcaagatccg ctcctttgtt cgaagggctg gaagaactac taatgctcaa tctagggcaa
     1261 tgaatgagtt agctcctcaa tttctacttc cttttaatgg taagttattt gattggtcta
     1321 tttttgattc attgtccagc ccagatcaaa atcaattccc gcagcgtatt cttganngca
     1381 ttgcttaaga aaattggcga gttgcagtta agtaatcttc gcctgattca gcacgatgcg
     1441 gtcgaggtgt tggagcacat gattgctaga gagtctttgg atggcgtgca tatattcttt
     1501 ccggatcctt ggcataagaa gcgacatcat aagcgtcgct taattcaaga agactttatt
     1561 actcttttgg tgtcacgtct taaaagtggt gggtatattc atttggcaac agattggcat
     1621 cactatgccg agcaaatgtt gctagttcta aatcaacacc ctaagctcga aaatagctct
     1681 aagcagttgg tgttactaga agcaatagaa ctcagcaatg ccgagcaaga aatcggtgga
     1741 atagtattta gccacgaaca cttagccaag ccgcacgctg gctatgtgga gcggcccgct
     1801 tatcgaccca tcactaagtt tgagaatcgt ggccttaaat tgggccacgg ggtttgggat
     1861 ttactctatc aaaagagata actctttata gcccaataca aacgtatttc atttcgaggt
     1921 actcatcaat gccccaagag cttccctccc gacctagacc ggattgtttt actccaccaa
     1981 atggggcgac ttcgtttgag atcagaccgg tattaacacc caccatccca aattcaaggg
     2041 cctcagcgat cttccaaata cgaccaatat ccctgctata gaagtaagaa gccaatccaa
     2101 attgactgct attcgctagt ttcacgacat cctcatcgct ctcaaaagga ataactggag
     2161 ccaccggtcc aaacgtttct tccacggtga tgagcatcga gctattaaca cctgctagta
     2221 cggtaggctc atagtaggtg ccacctagac tggagcgctt tcctccaatg acgagacgcg
     2281 ctcctttgct gagtgcatcg gccacatgcc gttctacttt ctcaatggcc tgttgatcaa
     2341 tcagagggcc ttgtgagatc ccttgttcaa gaccattacc aatcttgatc gatttagtag
     2401 ctgtcgccag cttttccaca aaaatatcat ggaccttttt gtgaacatag aaacggttgg
     2461 cgcatacaca ggtttggcct gcattgcgat atttggagac cattgcaccc gccaccgcag
     2521 catcaatatc agcatcgtca aacacaataa atggcgcgtg accacctaac tctagagcta
     2581 gtttcttgat cgtaggcgca gattgggcca ttaagatgcg accgacctcg gttgatccag
     2641 taaacgaaag gtggcgcact gtgggggatt cgcacagcac cttaccgacc gcaatggatt
     2701 gttctgagtc tgcggtcacg atattaatca ccccatcggg aaccccggcc tgttgggcta
     2761 ggtaggcaat tgccaaggct gatagggggg tttgctcagc aggtttaatc acgatcgtgc
     2821 aacccgctgc catggctggc gcaattttgc gagtgatcat ggcaattgga aagttccagg
     2881 gagtaatggc cacacaaacc ccaatcgctt gtttaaggac gatggtgcgt ttatcgcccc
     2941 aggtgcttga gggtatggcc cccattacgc gtttggcttc ctcagcaaac cattccacaa
     3001 aagaggcccc gtatacaacc tctcctttag cctccaccag aggcttgccc tgttccaatg
     3061 tcatcagggt tgctagatca tcggcgttag ccaaaattaa ttcaaaccat ttacgcataa
     3121 tcccagcgcg ctctttggca actttgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnccattg atcagggcat cttgcctaaa tagggcggga tttttaagaa gcgtggcggg
     3241 gttggcaatc gattctgggg cgttcatggc taggcggtct cgttgagtaa ttatggataa
     3301 gttttcaagt gtaatacctt ggctttcaag gacagagaac tcatttcatc caaatcgatt
     3361 gcaatctgaa agtcccaatt acctattaat ttattgttct ctgtatagct aatataaaaa
     3421 cataagcact actagcggta gtaggtaagg tggaattgac atcctatagg tagttttaaa
     3481 tagacatttt ttctttcaaa ttatgaaaat aactccggca atttggtgtt tttcatgatg
     3541 aggaataagt gatgactaac cactctaagc ttatgaatta aatgaatatt aaatttatag
     3601 cactgcatca tattagggtt ttaactgatt actgcttata aaagtaataa aaatacactt
     3661 ctttatgctt gacaggttat atagggattc ctaagatgcc acatgtttaa taaatgttgc
     3721 gttgcaatat aactgaatat ctaaacagat atttctttgt aatgcagtgt gaccctgaga
     3781 atgaaccaat atatttttga caagttgaaa gttctagatc ttcacaagat ggatcttttg
     3841 gcggccttac agcgatgggg tcagtcgatg attgccttgc tagtggccgc tgttttggct
     3901 gtctggctga ttgggcctaa aaccggtgcc gggctttttg atctagccca ctatttagta
     3961 ccagccgagg cgcgggcatt aatcttaggt actgatagtg cagaaatact aactgctgat
     4021 gagtccgccg gccaactcaa gctatggaat tctgctgggc gtgatattcc ttcgatcgct
     4081 agtcaggctg ctttaattcc gtctcactta gtcgatcact ccgctatgga caggggtatt
     4141 ttgcgaacag aggctgagag aaaggcagtt gcatcccatt tagctaagaa gtttcagtta
     4201 tctccagaag atacggctca ctatgttgcg caagcgattg ctgtcggcaa ggaagtcaac
     4261 ctagacccta ccttaatctt ggcagtgatg gccaccgaat caagctttaa cccccgggca
     4321 gagagtcggg ctggcgcaca aggcctaatg caagttcgca cccacgttca tcaagagaaa
     4381 tttgagccct atggcggccc tctggcggca tttataccgg aggcgaatat acgcgtaggt
     4441 gccttgatcc taaaggcctg tatcgcaaag gctggatctt tagaagcggg tcttaagaac
     4501 taccttgggg caccaaatgc tgctagtgga atcaatacct acactggtaa agtattttct
     4561 gagcgggaag aattacgcag tatcgctcgg cgctttagca ataacgttta atacagaacg
     4621 gcaaaagatt tcgctattct gatttagctg cttgtttcag catgnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccccca aaggttttgg
     4741 ttagctcaac agcttcattg atggctactt tgtaggggac tgacaggtcc gctgcgagtt
     4801 cataggcacc aattaagagg gcagcatgct ccacaggaga aagctcagca atgggccgat
     4861 cgagctctgg ggtaatgaga gcatccagtt cttgactgcg attcatgact ccttggaaga
     4921 tgcttgagaa caaatcctgt tggcatttct gaaaagcagg atcttcgctc aattgcttgg
     4981 taacagcact aatcgccaag ggaagatcat tcagctcgcc agcaatttta agaaccaagc
     5041 attgatagac gccctgaagt gcgtattcgc gcgcacgacg gcgaggtgtg agtgagcgct
     5101 tgtgcgcccc agtatttgct gttttattac tcaagcaatc actccgactc aatttcaata
     5161 tcaggagtaa gtgctagagc aaggtttgcc atttcaacag caactctggc acaatcgcgc
     5221 cctttctcac ccgagcgagc agtcgcttgc tcatcggtat cgcatgtaag aatgccattc
     5281 gcaatcggca caccggcatc taggctaatg cgtgtaatgc cactagcaga ctcgttagaa
     5341 actaactcaa agtgataggt ttccccacga attaccgcgc ctaaggcgat gaaggcatca
     5401 aactcatctg tttttacgag ttgagaaaga gcaaagggta tctctagagc gccgggaacg
     5461 gtaacaagtt gtatatcggc atgggaaaca cctaaggcaa gcaattcatt aatgcacgat
     5521 tctgacaggg cttggcagtg ctcttcatta aaacgcgcct gcacaatagc aatccgtaga
     5581 tcctgtccgt ctaagtctgc ctcaaaaaca tcgatgtcat tcatggatgg tcccttttgg
     5641 atagattggc agagtaggga atgttcccag taatttcaag tttatagcca gatagacttg
     5701 gtacacgagc agaattagcc aagaggcaca tcttttgaac ttgcaaatct ttgaggattt
     5761 gggcaccaat gccgtaactc cgaaagtcgg tctttcgttc aatgctggat ttagtgttga
     5821 cacttgacgc cttattgttg acatctgtag cttgttggca cagcttatca aattgctgta
     5881 gccacttcaa ttcagagggt gctgcaattc cagcagcatt gagaagtacg gcaaccccgc
     5941 ttgtagattg agcaattgcc tctagtgcct tactgagagg ccaggagtgc gtggagctct
     6001 cggtttccag aagatctaaa atggtgactg gctcgtgcac ccgaactaag acctcttgat
     6061 taggctgagg ttgtccttta atcagtgcca ggtgcaaaca gttgctcggt ttgtcgcgat
     6121 agaccacccc ctgaaattta ccccaagggc tcataaactc gcgctcggat tcgcgcacca
     6181 caatactctc tgtttgactg cgatactgaa tcaggtcggc aatggtacca atcttgagtt
     6241 gatgcgcttt tgcaaactct aggagatcag gcaggcgcgc catattaccg tcatctttca
     6301 tgatttcaca aatgactgcg gtcggagagc aaccagcaag actggctaga tcacaccctg
     6361 cttcagtatg accggaccga attaaaacac cgcccggttg agccatcaag ggaaatacat
     6421 gtcctggctg aactaaatca ttgggcttag catttggcgc tacagccgcc tgaattgtgc
     6481 gggcgcgatc agctgcagag atacccgtgg tcacgccgga ggctgcctca atcgagacgg
     6541 taaagttggt cccaagggca gtgccgtttt cacgaaccat taaagggaga ttaagttgct
     6601 gacaacgctc tttacttaag gttaaacaaa ttaagccccg tccgtgcttg gccatgaagt
     6661 taattgcttc tgcattgaca tgatcagcag cgagcactag atccccttca ttttcacgat
     6721 cctcctcatc gaccaagata atcatcttgc cggcgcgtag ctcggcaacg atttcttgaa
     6781 caggactaat ggcaacagat tgagggtttg gcatatcagc ttagattcta aatagatcgc
     6841 cattttaagc ccttaggctg ctttgtaggg gaatacaaca gaacgatccg taaagcctga
     6901 ttttggagtg ccattcacta aagcagaatg aatgggaaca ttcaatttct taaatcagac
     6961 aaatcagtga agtcggtatg aaaaagttta gtaactgtaa tgaaaaaggc tttgtgtttt
     7021 tagagctttt aattgccacg accatcttgg caggtagcat cacggctttg cacnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttaactctc agcgcagtca tgatctactc
     7201 accgcttaaa ttattgagcg atgtattact gaaacaagcc gagattgaag aagatatcct
     7261 tttaaaccag aatatgaatc gtgccatgga gttattgatg agggctattc gtgaagcagg
     7321 atatcgctct aaccagaata gtaaaaatga gaatgatatt cagattaagc aaggtggcct
     7381 atttcgcgga tcggatgcaa tcgagttgat gcaagatctg cccaagcatt tagcatatga
     7441 ctgtatggga aacattcttt ctaaggaacg cactcgtcat cgaaaaacgt atcaacactt
     7501 ctacctcgag cgaagccgta atgatccacg tagcgccatt ctgatttgtc agagtctaga
     7561 tcgtcaaggg cgtttacatc aagcagagct tctcaatcag gtgcaatatc ttcaaataca
     7621 ttgggtcaat cctcatgcag tcgatatcaa tagcgtgaaa cctaaagtag cctctggatt
     7681 gatcaaaatc agtcttgggg tagagcgtaa atcacaggca ggtaagggaa ataccttgat
     7741 cgagcaagtc cgtcttgttg ctccacgaca tatatgaaag cgccctaatg atattgctat
     7801 gggtgatgag tattttgatg tatctaacat gggcaatcac tcagcttgag cagtatttag
     7861 tattggagct caaaacgatc gctgtctacc agggtcattt ggaggatttt cagaagactg
     7921 agcatgtatt gctggcttgt gaacaaagtc tcctcgaacc tgcttcacta gagagctccc
     7981 aattaaaaat aacttactct caatcacctg ctaagagcac tccaatgcca tgcatgatcg
     8041 aggctagtgg tcttgttgaa ccaagaaaag gatcgagcaa aaatgtaatc caaaacccaa
     8101 agcgccttta tcacgttagg gtaggggatc gcattcgtat ggaaagtacg atagttgtgg
     8161 atcatctagc tggaacgttg acacgactca attggcaaca gctctatgat taacagggac
     8221 aaagaaaaaa tatctatcaa gtctatatcc cagcaaggcg gttttactct ggtagagtta
     8281 ttagcagtga tcgccttatt aagtttgatg ttttttatgg gtctaccgct tata
//
LOCUS       DDPQ01000056            5838 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_19197,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000056 DDPQ01000000
VERSION     DDPQ01000056.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_19197"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1042..1081
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1723..1757
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1554 a         1167 c         1448 g         1590 t
ORIGIN      
        1 ttgaaccgaa tgctagatgg cttacagggg gagggtgagc cattgccatt aattttatgg
       61 agtgtgagcg atgagctccg aaccctccat aaggtacagt ctgcattatt ggctggtgat
      121 gccctagcaa acttattacg gaactatcga atttggggaa agcgcgagaa gttgtatccc
      181 gttgcactta aacgtattgc acctaaaaaa ctgaagcaag ccatggtttt ggctgcgaac
      241 cttgataaac aagtgaaagg cttattagtc cgtgacttac ctagcaatcc atgggatggt
      301 cttcgcctga ttggtggctt attgcgttag agtatggtct atgggagcac aaaaccaatc
      361 cacgattgag caaatgatgt tggctattgg ccagcaagcg agatccgcat cgcgggcgat
      421 ggcaaaagca agtagtgagc aaaagaatcg ggcattaatt aatattgcga cagctgttcg
      481 tcgggatgct gccaaaatat tggcggctaa tgcggtcgat ataaagcgtg ccaagactaa
      541 tggtaacgat gcagccttta ttgatcggtt gaccatgacc gagaaatcca ttgagaacat
      601 ggctttgggc cttgtgcaaa tcgctagtct gggagatcct gttgggcaca tcggtgcttt
      661 taagaaacaa gcctcaggaa ttttgatcgc gcagatgcgc gtgcctttag gggtcattgg
      721 tattatttat gagtcaagac ccaacgtgac tattgatgct gccgcactat gtttgaagtc
      781 aggcaatgca gtcattttgc gaggcggttc agaggcaatt gattcaaata cctacttggc
      841 aggtttgatt caggaaggat tggctgcggc acaacttccc gcagaagctg ttcaggtggt
      901 tcaaacaacc gatcgagccg ctgttggcaa gatgattacc atgaccgaat acattgatgt
      961 catcgtacct cgtggtggta agagtttgat tgcgcgttta atggcggatg gtcgagtgcc
     1021 catgattaag caccttgatg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nccatgtaat gcgatggaaa ccctattggt caatacgaag atcgctaaag aggtattacc
     1141 acctctttgc aagatttatc aagataaagg cgttgagttg cgagttgact ctaaaactcg
     1201 caaggttctg gagaataatg gttttgttca tttggttgac gcaagcgaag aggattggca
     1261 cacggagtat ttggcaccca tcttatcgat taagatggtt gaggatttag atgaggcgat
     1321 ggatcatatt gagcattatg gtagcaagca tacagatgcg atcattacgc aaaatcaagc
     1381 ccacgccgat cgattcttac gagaggtcga tagtgcgagt gtgatggtta ataccagcac
     1441 ccgctttgct gacggctttg agtatggcct tggtgctgag attgggattt cgaatgacaa
     1501 attgcatgct cgaggccccg ttggattaga gggccttaca tccctaaagt acgtcgtcat
     1561 gggccatggc gaaatacgac aataaattat caatttaaag agaagctatg tttgatggat
     1621 acctttgggc taagaccctc catattgtct tgattgcttc ctggtttgcc ggtttgtttt
     1681 atctaccccg tatttttgtg aacttggcac aagaaacaaa tcnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnntag gcttggtatt gtggctttat tacggcgtgg gtagaggctc
     1801 tatttggatg catataaaag tcctcttagt gctggtaatt attgcctatc atttgcaatg
     1861 taagaaatta ctaaagagct ttttagaaaa aaccaataca aaatcacatg tatggtatcg
     1921 ctggtttaat gaggtacccg ttttactcat gctagtagta actgcacttg tggtgatcaa
     1981 gcccaattga ttttatctaa gagcgaactg aactcaccat gaaatttttt attgtttgtc
     2041 caggtgggct agagagtgct ttggcgcgcg aacttgagga gattgcaaag cgagccgagg
     2101 taaagacctt gggggctact caaattgacc ctgttcctgc caatatgaca ggtggtattg
     2161 gcgtcaatgg tccattagca gtcgcgatgg caatcaatct acattctcgt attgctagcc
     2221 gcgttttact gaaaatggct gatggcccct ataagactga ggatgattta tttcgtttgg
     2281 ccaaatcgat tggatgggag gattggttta gttctcatca aacgcttcgg gtagacctga
     2341 ccgcacagcg ttcaccctta aagagtttga actttgcgac ccttcgtatt aaagatgcca
     2401 ttgtcgatcg tttgcgggag caaacgggcg agcgccccaa tatcgataca gctaatccag
     2461 atgtgcgtgt acaggcccat ttaaccactg atgaagccac tatctacttg gatacatcag
     2521 gtgagccttt gtttaaaaga ggttggcgcg aggagaaagg agaggctccg ttaaaggaga
     2581 acctggctgc agggattttg gcacttactc catggcaaac caatcaagct ttatacgacc
     2641 ccatgtgtgg cagcggtact tttttgatcg aggccgcaca aatggcgttg catattccgc
     2701 caggggcaat acgtgcacag ttatttgagg ataagaatct atctgcgtca acgaagcttg
     2761 gtggctggaa acaagcggta gagctttctg gatttggctt tacccgatta aagccattta
     2821 atacctctct cgagaaaaag aactgggctg gtctttgtga tatctcaaaa aaagagatag
     2881 caagttttgc aaagtttccc attgcaatta gtggcagtga tatcaatgaa aagatgatca
     2941 cgatttgtaa ggctaattgg caaagggccc atttgccagg gttgccgaat gtgcgacagg
     3001 tcgatgctat tgcagtgaaa cctgcttcag accaaggtgg ggtgatggtg ttaaaccctc
     3061 cttatggaga gcgcttgggt attaaaggcg gagatcaaga caaaatatat ccattgggag
     3121 atgaggataa atcttatatg caaaagcgtc gcactagtcg tgagccctta gtcaaagaac
     3181 ctattgatcc tcaatttgct gcatttttag atcaatttag caaacaatta aaggaccact
     3241 ttggtggctg ggaaatttat gcattgacgg cagatatggg cttaccaggg cacttgcgga
     3301 tgaaagaatc caagcgcacc ccattgttta atggcccttt agagtgtcgt ctgtttaagt
     3361 ttgatattcg aaaaagagat tagtcaggct tggctttaga atagaaatct ttaatagaaa
     3421 gaaaaaagtt ttatggaatt caagacctat atgtgtttaa tttgcggttg gatctatgac
     3481 gaatctaagg gctgtccaga tgacggaatt gcaccaggca ccctctggaa agatgttcca
     3541 atgaactggt cttgtccaga gtgcggagcg cgtaaagatg attttgagat gtcggtaatt
     3601 tagtctgcag atccacaaaa ccatttcact taaatatcat gagtcaaaac gagcaattat
     3661 ttaatcgtgc ccaaaaaaca atccctgggg gagtgaactc tccggttaga gcttttcgtc
     3721 aagtcggcgg gataccccga tttgtggcac gagcagaggg gccttacttc tgggatgccg
     3781 atggtaagcg ctatgttgat ctcatcatgt cttggggccc aatgattgct ggccatgcca
     3841 attcagaggt tgtcgaggcg gttcaaaaag ctgcaaccca aagttttagt tacggggcgc
     3901 ccactgctgg cgaaattgat ttagcagaac acatttgtga gttgatgcca aatattgagc
     3961 agatccgcat ggtttccagt gggaccgagg caacaatgag cgcactccgc ttagcccgag
     4021 gctataccaa tcgtgatctn ntgattatca agtttgaggg ctgctatcac ggccatgctg
     4081 acagcctatt agtgaaggcg ggctctgggc tcctgacctt tgcagactct actcagaacg
     4141 ccccttcatc aggaggcgtt cctcaagatt tagtgaagca caccttggta ttgccatata
     4201 acgatgttgg tgcacttgaa gaggcattta agagacatgg tgatcaagtg gctgcattaa
     4261 ttgtggagcc aattgccggt aatatgaatt tgattcgtgc tacacctgag tttgttaagg
     4321 cgcttcgagc gcttactgaa aaacatggtg cagttctaat ctatgacgaa gtgatgaccg
     4381 gatttagagt cgcccttggc ggtactcaat cattacatgg cattaaacct gatttaacct
     4441 gtcttggtaa ggtgatgggc gggggaatgc cgatggctgc gtttggtggg aagcgcgaga
     4501 ttatgggtaa gttagcacca ttgggtaatg tctatcaggc ggggacgctc tcgggtaatc
     4561 cagttgccgt tgctgctgga gcaaagactc tggagattat ctctagagct ggattttatg
     4621 agtgcttaag tgagcagaca aaaaaactaa tgagtggctt aaagcatgaa gcagatttag
     4681 caaagatacc cttctcagtg gatagtgtgg gcggaatgtt tggcttttac ttctctgacc
     4741 aagtacccac ttcttatgaa gaagttacca aaacgaatat tgagtccttt aagcggtttt
     4801 tccacgccat gctcgatgct ggtgtatatc ttgcaccatc agcctatgag gcaggtttta
     4861 cttcgatagc acatgacaat aaaattgttg atgaaattat taccgctgcc aagaaatcct
     4921 tcgcacagct aagataatca ttacgaaagc attacattcg cattgggtga tcgtaacctg
     4981 caacgcttag tgttttaatc tttaggcctt gatcagtggg gggtaatctt attatctcat
     5041 ttgcgcttgc taaatgatct agatcaattg ctgttaatgt gccaccccaa acgcagcccg
     5101 tatcaatccc tacgacacga tgccgattta gtaggcccaa ggtagaccaa tggccaaata
     5161 caataggcat ttcttgtgtt ttgcgtccag ggatctcaaa ccaagggata tatccagcgg
     5221 gaccgctttc tagaccctct ttacttttga actccatctc accctcgggg gtacaaaaac
     5281 gaactcgggt cagagtgttt gtaataagac ggagacgatc aatccctttg agctttggat
     5341 cccagtaatt tggcgtattg ccatacattt gtgccaagaa ctgtttgtat gtcttttggc
     5401 gaagcgcttg ttcgacttca gcagcaagtt ctagggtgcg ctttacactc cattggggga
     5461 gtactcctgc atgaacaagc agcacctttc cattactcaa agccataggt cggtgacgca
     5521 accaatcaat taactcttta cgatcgggtg ctcgcaaaat atcatcaatc gtatcgagcg
     5581 gcttagaatc ccgtagaccg gcatcgcagg ccagaagatt gagnnccagt ttcttgaaga
     5641 tgtttaaggc gacgtagact tcccaaagaa tctggtccac gattaatcat atcgcctagg
     5701 caaataatct tagaagtgcg aggtaatttt ttgatcagtt gattaagcga acttaagcag
     5761 ccctggatat cgccaattgc aaatacgcgg gacatgctca ggagaccttt ttgaccacgt
     5821 tataccgaat caaggtag
//
LOCUS       DDPQ01000057           11256 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_1543,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000057 DDPQ01000000
VERSION     DDPQ01000057.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11256)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11256
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_1543"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1334..1357
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2530..2584
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9677..9702
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3121 a         2414 c         2692 g         2924 t
ORIGIN      
        1 aagccttgga cgctaccata ttcatttaca actaagctca agcgttgacg gttttcctga
       61 aaaaattgca tttgttgcat gaccggtgtg ccctcaggaa taaaatacgg atcattcagc
      121 aactctctaa aatcagcatg ggtaagttct ttgttaccca aaagtgacaa agctttttta
      181 accgagagaa tgccaatgat tttctctgga tcaccatcgc atacaggcaa tttattgtga
      241 tagcaggttt ccagctgtcc aatcacttcc tcaatcggac gagatagatc caagatttcc
      301 atcttggctc ttggggtcat aacatcgctc acaagaatat gctcaagatt aaaaagatta
      361 agcagaatat tgcgatgctg tttggagata aagtgactag actcaaggac cacgctcctt
      421 agctcctcag cactcatggt ctgtaagttt gcttcctttt gattcagccc cataaggccc
      481 attaatccag aaacaaagga gttcaccagc cacaacaggg gcttgagaat aatagttagg
      541 ggataaataa cccagccaat cttgaccgat attttttctg cgaatgctgc accaatgacc
      601 tttggcgtaa tttcactaaa aataatgatc aaaaaagcaa ccgtcagggt tgcaatagat
      661 agcacaaggc catttgtacc aaataaatga agtgcaattc cagtaaccag aataggcaga
      721 atggtattaa ttaaattatt tgcaattaat aagaccgata gaagtgtgtc aactcgtttt
      781 aggagttttt gggtgatggc tgcgccggca tgtccgctgt tggccatggc acgaaggcga
      841 tgccgattgc ttgcgaccat actggtttct gccatcgaga agaatccaga taaggcaagc
      901 aaaaatacga tgagtgctgc ttgtgcccat aaaggccaag agtcaaagaa tgcgtccatt
      961 aatactctca aaaaaggtac aggagatcaa tatagcaaat accgattttt ctagctatta
     1021 ggggatattg tttttgctgt cagattaaga ttacagatat gacccgttct agccataagc
     1081 aacccattca tattgaccga tgtgttatct cctccccctt tggaagcctg cgtattagta
     1141 ccgaattggt ggatggtagc ttgatgattt ctgaaataag ctatcttgct aaagggctca
     1201 gtaaagtacg cccccaaaat gatttggcgc ggcaggcaaa agatcaaatc gaagcttact
     1261 ttgaagatcc tttatttaag ttcaatctac cactaaagca acaaggcacg ccatatcagc
     1321 accgcgtttg gtcnnnnnnn nnnnnnnnnn nnnnnnntgt ggtgcaaatt attacccgct
     1381 tttaatacct tgccatcgag ttttatcagc taatggattg ggggggttca tgcaacaagg
     1441 tggtgaaatc ggtcggcacc tagatatcaa gcgctggctc ttgcgccatg aagggataga
     1501 atttattaag ccttgagcgc cctagcctga agtcgaaaaa acagccccat caggaaatca
     1561 ctgcgtacgg caagtttcgt aaagatatag aagcgactaa cttgcccacg cgtgagtttc
     1621 acctcagcgc ccccttggag cgtttgccgt tgtgcaattt gtttaagggt cataactgca
     1681 agcccaaagg ctaagaaaca aaattggcga atacccggct ctgatttagg aatacagcag
     1741 atatagcgca gggagtcctc tagtttttga tgggctatga ccaataaatc ggtctgacca
     1801 aggcctttag gtttccagga cacccccctt aattgatctt ccggagagtc ttttaagata
     1861 tttgtcattt gtagggcctg accaaacgca atcgctaaac tttctttccc agagatggct
     1921 tttgcaaatg ctggggagtg aagtgcaaaa agagtggtta gcatttcgcc tacaactcca
     1981 gctacgacat agcaatattt ttcaaattcc ttaaggtcct ctagaccact ttgattctgg
     2041 cgtatatgaa aataggacat tccttcagac atgatttgca cacatttagc aactgcatga
     2101 cgttgtttct catcacagct ttgtaagacg cgtaagaccc ttggaatatt ttcaataaga
     2161 tctttttcat tatgattagg atgattaata agagcgatca aacattcccg aacaaattgt
     2221 tctgaaagac tatgatcaag taccgttttg ataaataaag tagatagctc ctgctttgtt
     2281 gtcggatcaa cacaggtagc atcctcaatc gtatctacga tgcgacacaa aagataggta
     2341 ttgccgacta ctttttctaa gccattcggc agcagcggaa tcgtgagtgc aaaggttctg
     2401 gaaacggact caagaatgga gcgctggtag agttcatcat gcgctagctg attgaaggag
     2461 gaaggggggt cgctaatact ctgaggcatg ccgtcattat gtcaaactgt tttatagcaa
     2521 ggtagcttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnaccatc ccaaatagac tggcgatgag gccaaggagc tgaggctcaa ctggctcctc
     2641 gatcccagtg atctccagaa cgatccagac gcttagccca aagaaaatag ctagaagtgc
     2701 cccaagattg ctggcccttt tccaaaagag gccggccacc aagggaacaa aagctcctgc
     2761 tagggtaatt cgataggcat tctcaaccat cgtatgaata cgggaatcgg tcacgaaggc
     2821 gtaataggta acaatgcttg tgaatacaaa aacggtcagg cgggttgccc ataagaactg
     2881 cttatcggac atgcctggag agaagccttt cagaatgttt tctgtaaagg tgactgatgg
     2941 agctaataat gtgcctgagg cagtactcat aatcacagaa atgagcgcgc caaagaaaat
     3001 gacctgaata aagaatggca tgtgatttaa gatgagtgtc ggcaaaatca tttgagaatc
     3061 ccctgctaga tatttctcaa ccatagcagg atcaataatg ttggctgaat aggctaaaaa
     3121 gaggggtact gctgcaaaaa agaagtaggc gcaaccgccc aatatggttc cccaaactgc
     3181 cactgcttca ttcttggcag cattgaccct ctgaaaaaca tcttgttggg gaatggagcc
     3241 tagagccatc gttagcaagg ctgcgatcca gcccagcata tcaagtagat ctagctttgg
     3301 gaggaagtca aatttaccct ctgcgctagc ctttgagata accgaagcaa cccccccagc
     3361 ctgctcagca gcattgctag caaccaataa aaggccaatc acaatcacaa ccatttgcac
     3421 aagagtggtg agagctacag accacatgcc gccgaataag gtatagatca atacaactgc
     3481 ggcaccgatt aacatgcctt ccttggtaga aattaaatcg tttgatagaa cattaaaggt
     3541 aagtccgaga gcggtaattt gcgctgatac ccagcccaaa taagaaacga tgatgcaaat
     3601 cgatagcaat aactcggttt ggcggttata gcggattcga aagaagtcgc ccagagtaat
     3661 caggttcatt cggtagagcg gacgagcaaa gacaagccca aagagaatta aacaaattga
     3721 cgatcctaag gggtcagaga tcaggccacg aaagccttcc tcaagaaaag tagccgaaat
     3781 gccgagaacg gtttctgcgc caaaccaagt tgcaaaaacc attgataaaa caaacaccat
     3841 gggcagatta cgtccagcgg taatatagtc cttagcgtta tgtactttgg tggcaccata
     3901 aagaccgatc cctaccgaga caataaggta ggcaatgact atccaaagta gcatcagaaa
     3961 ctcctatcgc gacaactaat ctagtgactt atcggccagt ttataactag ccctaattat
     4021 aggaggggat tagcttctgg gtcactaaat gtgccgggcg agcctttatc tcaaagggga
     4081 ggtattcaaa aactagccga accctgattt tttcatccct cgatttggac cctactttta
     4141 tttaagggcg ggggacttat agaggcaata tcctgaaaaa atatcgtact attgtcaaag
     4201 ataaaggttt ctatttagcg agggcgggtt atgaagattt gtattattgg cggcggcggt
     4261 gcgatcggag gatatctggc ggttcttttg gccaacgccg gtaatgatgt gacggtcatg
     4321 gcgcgcggcg caaccttaag agcaattgaa gaacgcggct tggccctcat tagtgatgag
     4381 catccagagc ccataattgc caaagtaaag gcggttgaga atcttcgtgg tttgcctaag
     4441 caggacgtca tcattctggc agtcaaagca caccaagtgg agccggtcat tgatgatctt
     4501 gtatcggtct taagtcccga aaccgtcttg atcccgatgc aaaatggtat tccttggtgg
     4561 tatttccaaa aactgaatag caagtacaaa gatcactccg ttgataccgt tgatccgggt
     4621 ggcgtgatta tgcgtgcaat tagccctgac aacatcatcg gttgtgttgt ctatccagca
     4681 acgatcaccc agggccctgg tgttatacgt cacgtagaag gcgtccgctt tcccttggga
     4741 gagctcgacg ggaaaaccac agatcggatt caaaaaattt ccgatgttat gggcgcagct
     4801 ggatttaagt cccccatttt ggatgatatc cgtgcagaga tttggttaaa gctatggggt
     4861 aatctgacat tcaatccaat tagcgcactg acgcatggaa ctcttgaggg aatttgccgc
     4921 taccccttaa ctaaagagtt ggcacgcaac atgatggcgg aagcacaatc cattgcagaa
     4981 aaattaggcg tcacgttccg ggttgatatt gaacgacgaa ttgcgggtgc ggagaaagtt
     5041 ggcaaacata agacttctat gttgcaggat ttagaggcag gccgttcctt ggaaattgat
     5101 gcgcttctag gttcggttat cgagttagga gagattaccg agacgcccac accttgtcta
     5161 aatacagttt atgcattaac aaaatattta gatcaaaacg tgcaagacgc caaaggtagc
     5221 ctcatacttc cagttgctgc aggttactaa tttagattta ttcaaagcgg ttatcaagtc
     5281 gaccaacgcc atcgatcacg atactgacat tactgcctgg tttcatggat ccgacaccta
     5341 ccgaggttcc gcaggcaata atgtcgcccg cttccagtgt catatcttgg gatatcagac
     5401 ttactaattt agctggtgag aaaacaatat cgctcaaggg atagttttgg cgttcttgat
     5461 cattgaggat ggttttaata cttaaactca tcggatcaat atcagtacta ataaccggac
     5521 caaagacccc aaaggtatcg aaattcttag agcgggtcca ctgagcataa ccagggttgc
     5581 gattcaagat ttcaaccgca gtgacgtcgt tgacgcaggt atagccaaag atatgtttag
     5641 ctgcgtccgc ctcactcact ccatgacagc gcccgccgat cacaattcct aattctcctt
     5701 cgtaaatcac tttgccgcta taggatttag gaacccgaat tgtttgtcct gcagcaagat
     5761 acgaattatt tgatttgaga aagtaaaggg gctcatctgg aacggcgtgc tctagctttg
     5821 tcaccagagc atgaaagttg tcgactagag ctaaaaactt attggggacg cagggaatat
     5881 caatgatcac atcttttaat ttgaggtact caccagtggc gcgaggattt tggaataaat
     5941 caccgctatg gaccgcaatt tgatcccctt gcaattgtcc aaagccagaa attccgtcgt
     6001 gccgataacg taaccattgc gccataataa gatactccta aagtagtaat tactaggatc
     6061 ttatcaagga cttgagcgtt accctccaaa tcatggcaga actcttattt gcacccgaat
     6121 acgatcagcc tatttcgtat attcaacgca ctcgatcgta ttacctcggg ttggggtatg
     6181 acaagcccta tgtttgggcg cattacaccg atgttccctt tactcccctt caaaaacctc
     6241 ttcagaattc agtcattggt ttgattacaa cagcagttcc ttttgatcca ttaaaggggg
     6301 atcaaggccc gggtgcaagc tataacgcac atgctaagtt ttatgagccc tatgctttgg
     6361 cgagcaagag taatcctgat cttcgtattg cacatgttgg tatagatcgt aagcatgcaa
     6421 atatggctga gatggcatgt tggtttccgc tagaagtggc aagacgggca gcagattgcc
     6481 aacggattgg taaggtggcc gaacgtttct atggtttgcc gaccaatcgt agtcagcgcc
     6541 acactcttga aatcgatgcc cctcaaatct tggctaattg cagggacgat ggggtcgatc
     6601 ttgctatttt ggttccaaac tgtccgattt gtcatcaaag cattgccctt ttagctcgct
     6661 accttgaagg ggctggtatt cctacggtcg tcattggtgc agcaaaagac atcatcgaat
     6721 attgcggcgt tcctcgcttt gtattcaatg actttcctct gggaaacgcc gcaggacgtc
     6781 cgggagatcc tgagtctcaa aatgctattt ttgagctcgc attaatgctt ttggagcggg
     6841 cgccagcgcc ccgtacgact attcaatcag aatttccctg gagttctgac ccaagttgga
     6901 aattagatta catgaatatt gagcgtttat cggatgtgga attggcaaaa ttacgtcttg
     6961 aggcagagac cgcacgaatt gccgccaagg agattcggct aaaaacggtt gggaagtaat
     7021 ggtcggaatg agaggattcg aacctccgac cccctcgtcc cgaacgaggt gcgctaccag
     7081 gctgcgctac attccgctaa ttattatttt atccctgacg aattaaggag tactcgcaca
     7141 gcgcacggag cgaatcactc gccgtatttt ttgggaagtc tttaatggtg tttaaggcta
     7201 gagccacttc tttgcgggca gcttcttggg tatattcaag ggcaccagat tcttgaatac
     7261 cttgcaaaat ttgtgcaaac acatcatcag gcagctcttc attttgagag attgcctctc
     7321 gtgctagtag ggattgttcg ggactaccct tttccatcag atagatcaga ggcaaagttg
     7381 gcttgccttc gcgtagatca tcacccacat ttttgcccat gtcttctgga ttggcggtgt
     7441 aatccagaag atcgtccatg agctggaagg ctgtaccaat gtgccgacca aaagctgccg
     7501 ctagctcacg ttcgatatcg ctagcttggg caatgagagc gcccagctcg gcagacgcct
     7561 caaagagttt tgctgttttg taacgaataa cctggagata gctcgattca tcgacttcag
     7621 gatcattcat gttgagaagc tgtaatactt cgccttccgc aatcgtatta gttgcattgg
     7681 agagaatttg catgacccga atatcatttg gggcaaccat catttggaat gcgcgtgaat
     7741 aaaggaagtc gccaaccaag acgctagcag cattcccaaa agcagcattt gctgtttgct
     7801 tgccccttcg catggtggac tcatcgacta cgtcatcatg caacagtgtg gctgtatgaa
     7861 tgaactcgac aacagccgct agctccaaag catggggaat ctcttttcca tgccctaggg
     7921 ccttggcaat caataacagt agagctgggc ggacacgttt tccacccgat tggatgatgt
     7981 aggcggctat ttggtcaatc aggacgactt cagacgcgag ccggcgtcgg attacctcat
     8041 ccaaagcctt taaatctagg ctaattgggg ccaatatttg gcctaagtta ttgtttttga
     8101 cagtattatt catccaagat ataatacttg gctcggctcg aactcaccga tttccccaaa
     8161 gtaactcgtt tcttagggcg ttttatggtg aaaccagtcg atttgtatct tccttatagt
     8221 attttttgag aggtttaacc atgtacgcgg tcataaaaac cggtggcaaa cagtataaag
     8281 ttgctgctgg cgaaaaattg aaaatagaac agataccggc ggacatcggc agcgatatta
     8341 ctcttgacca agttctcgcc gtaggcgagg gcacgtcgct aagaattggc gaccccttgg
     8401 tgagtggtgc cgctgtgatg gctactgttg tctcccaggg acgtcacgat aaagtgaaaa
     8461 tctttaagat gcgtcgtcgt aagcattatc aaaaacatca aggccatcgc cagaatttca
     8521 ctgagattct gattaagtcg attaaggcct gagtttagga gaaaaatatg gcacagaaaa
     8581 aaggcggcgg ctcaacacgt aacggacgcg actcagaatc aaaacgttta ggcgttaagg
     8641 tttatggcgg tcaagcgatc catgctggcg gcattattgt tcgtcaacgt ggcactaaag
     8701 tacatcctgg caccaatgtt ggtatgggta aggatcacac tttgtttgca ttgattgatg
     8761 gtcaggtcga atttggtgtg aaaggggctt tgaagaaggc ccaggtttca gtcttgcctc
     8821 gctcttaagc ggcctgactg agatttcttt ctactaagtt aatcaacagg aatgggcctc
     8881 gcaaactgcg aggccttttt tattcatgaa atttatcgac gaagcaaaaa ttgaggtcat
     8941 tgctggcaat ggtggagccg gtagtgcgtc gatgcgtcgc gagaagttta ttgaatttgg
     9001 tggcccagat ggcggcgatg gaggccgtgg cggcagtgtt tatgcgattg ccgatcgaaa
     9061 tatcaatacc ctaatcgact atcgttattc aaaaacacat ttggcaaaga atggtgagcc
     9121 tggtcgaggc gcagattgtt atggtcgggc tggcgatgat attgagttgc ggatgccagt
     9181 aggaacaatc atcacagatt ttgaaacagg cgatctcatt gccgacctaa caaagcatgg
     9241 cgaacgtcta tgtcttgctg aaggcggtgt gggcgggtgg ggcaatattc atttcaagag
     9301 cagtaccaat cgcgcgcccc gccagaaaac aaatggcaag ccaggcgtgc gccgtaagct
     9361 aaaacttgaa ctaaaagtat tagccgatgt cggcttattg ggcatgccca atgccggtaa
     9421 atcaacctta attacagcag tatcaaatgc taggccgaag attgcagatt atccatttac
     9481 aacgctgcat ccaaatttag gcgtagtgag agtgggcagc gaacgcagct ttgtgattgc
     9541 agatattccg ggcctcattg aaggggctgc ggagggggcc ggattaggac atcgttttct
     9601 acgacatctt cagcgtactg gagtgttatt gcatctagtc gatattgcac cctttgatga
     9661 gaatgtggat atcgttnnnn nnnnnnnnnn nnnnnnnnnn nncaaaagcc ccgttggcta
     9721 gtgctcaata aggtcgacat gttgcagcca gaggagcgtc agaagatcat taaagacttt
     9781 ttaaagcgct ttaaatggga tggccccgtt tttgaggtat cggccctaaa tggtgaggga
     9841 tgtgaccgac tttgttatgc cattcaggat tatttagacg gtcttcgtaa agagcgtgat
     9901 ttagaggagg agcgggccga agacccccgt ttcttggatg aatagtgcga ccttagaaaa
     9961 gatgaattgg tatgagtaaa agtattgcag gtacaaaacg aatcgtggtc aaagtaggct
    10021 ccactctggt tacaaataat ggagccggcc ttgatcgcca ggccattgca atgtgggccg
    10081 agcaggtgag tgctttatta aaacaaggct gtcaagtttt aatggtgagc tcaggagcta
    10141 ttgctgaagg gatgcaacgc ttggagtgga ccagccgccc tcaagagatt catcaattgc
    10201 aagctgctgc tgcagttggg caaatgggac tggtgcaagt ttatgaatcg tgttttacaa
    10261 aatttggcgt gcatacagct caagttttat taacgaatgc cgaccttgcc aatgcagagc
    10321 gcaatgcgaa tgccaaagca actttacaaa cgctattagg tttaggggtg gtgccgatca
    10381 ttaacgagaa cgacaccgtt gttaccgatg aaattaagtt tggagataac gatagtttgg
    10441 cggctctcgt tgtgaactta atttctgctg atgcgcttgt tatattgact gatcaaggcg
    10501 ggctctacag cgccgatcct agaaaagatc actcagccac tcttattgat catgcccgtg
    10561 caggcgatcc tcggttagag gaaatggctg gtggagccgg gagcagttta ggtaaaggcg
    10621 gcatgttaac taaggtgctg gcagctaaaa cggcagtata aacgggagcg agtaccgtca
    10681 tcgcatcagg caaagagccc aatgtgttag ttcgtcttta tcaaggcgaa tcgattggta
    10741 ccttcctaag caaataatta tttgggtatg gctattaagc cagtaaagtt atttggatct
    10801 aatgactgca ttactttgga gaaactcttc acttggctgg ctaaatcaca tagtgcacct
    10861 tgcgcaagtg ctgcttgata gcgattaggt acgctatcct taattggtaa ccattgaccc
    10921 aatggattgg tgcgccattg ttggttggtc tctaatgtac cgtaaagacg ccattgaaaa
    10981 gtttcacacc gaattccttc gaagtaggca ttttgtccac cactggggtt ggtaatcaca
    11041 acgatatagc gcgtaacacc atcattgcca atcttgatgg attcggtatc gatggcgaat
    11101 ttaaagacag tggtttgaga taccgagaac ggtactaaat attttttatt gggtggatta
    11161 agtggcaagg gggtcgaacc ctccttaaac accgtaggcg cataggggtc tgtaccatca
    11221 atcagtggat cagagaagca tccagcaagg gtaagt
//
LOCUS       DDPQ01000058           12708 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_224, whole
            genome shotgun sequence.
ACCESSION   DDPQ01000058 DDPQ01000000
VERSION     DDPQ01000058.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12708)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12708
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_224"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    4453..4539
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5939..5981
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3315 a         3269 c         2723 g         3271 t
ORIGIN      
        1 tttgcaaagt cgccaaacgg agcttcccaa atcgttaagg tattcggttc ggcagcggca
       61 tagccatact caaatcccag cacagcctct tccgacagaa tcgagtcgat gacagtaaat
      121 ggggcttgat ctttactcac atgctgcaat gggatgtggg taccagtgtc ccaattttct
      181 cgattttgcg catggagtac agagtgacga tgactaaatg tgccgcgacc actatcttca
      241 ccggataaac gaattgggta accgctagct agcaaggagg cgaaagccat tgcctcaccc
      301 atgccccaat ccacattgat ctcgccacga cccattgccg cacgatcggt atataccttt
      361 tgtaccaatg gatgtgcttt gaaatcatcc ggcagagttg ataacttatc tgcaagcctc
      421 ttccattcgg atagaggaat ggccgtgtca gcatgatcgg tccatttctt gttgaggaac
      481 ggtgaccaat ccactgcaaa cttccctttg aaattactca acaccggatc gagggtttgc
      541 ttgcccgcat ccattgcggc acgataggct ttcaccatct cgtcgcctgc gcctgctgca
      601 ataacacctt gcgcctcaag tcggtcagca tatagtttgc gggtaccagg atgagtagca
      661 ataatgctgt acatcaaggg ctgggtcatt gccggcgtat cttgctcgtt atggcctagc
      721 tttctgaaac aaataatatc aaccgcaaca tctttcttaa attgggtgcg gtactccaag
      781 gccagctgag tcgctaacac caccgcctct ggatcatccc cgttcacatg caaaaccggt
      841 gagtcgatgg ttttcatgat gtcggtgcaa taaagactag aacgcaaatc gcggggatct
      901 gaggtggtga aaccaatttg gttattgatc acaatatgga ccgtgccacc agtggaatat
      961 ccacgtactt cggataaagc caaggtctct tgcatcacgc cttgacctgc aatcgcagca
     1021 tcaccatgca cgagtaccgg tagaacttga tcgcccttcg catcattgcg acgctccatc
     1081 cttgcgcgtg ccgaaccctc cactactggg ttcacaatct ctaggtgcga tggattaaag
     1141 gacagggata agtgcactgg accacctggg gtcgagatgt cgctcgagaa tccttggtga
     1201 tacttcacat caccagcggg cagatcttct tgcgcggtgt gatcaaactc agcaaagagg
     1261 tctttgggtg atttacccaa tacattgact aagacattta agcgtccacg gtgcgccatg
     1321 ccaatcacaa tctcttggac gcctttcttg ccagccccct gaattaactc atccatgcag
     1381 ggaataaaac tatcgccccc ttcgagcgag aatcgttttt gccctacata ctttgcttgc
     1441 agataacgct caaggccttc tgctgcggtt acgcggtcta ggatctgacg acgttgctca
     1501 tttgtaaact taggcgtaga ccgaatcgac tctaatttct cttgccacca ttttttgatc
     1561 ttctgatctg caatgtacat aaactcagca gtcaaggtac cacaataggt ctcgcgcaat
     1621 gcctgcaaca gatcccgcag ggtcatgttg ttcttaccaa agaaggtatt gctggtgtta
     1681 aagacgatat ccatatcgcc atcactaaat ccgtaaaacg acggattaag ctccggaata
     1741 tcagggcgct ctgtgcgctt gagggggtct aaattagccc agcgattacc aacgttccga
     1801 taagcagcaa ttaattgctg aaccgcaacg cgtttgcgac ccatctccga gtccgctgaa
     1861 tcctggatcg ttttaatggg tccttgctta gcccgctcag caaaggatgc aacgattggg
     1921 gcatgggcaa tatctctgcc gtttgaacca tcgacagctg ggacttgaac aacgttatca
     1981 aaatagtcgc gccagtgctg tgcaacggat gtgggatcta ataaatagga ttcgtagagt
     2041 tcttctacgt agggggcatt acccccaaag aggtacgaag accctctata ggactgcatc
     2101 atgatgctca cctttcatcg ggtaacccga aaaaaactgt ggtttcaacc atctgcgaca
     2161 cggcttaacc gattagcaga acgcaaagca aatgccttat gtactgtaag gataacatga
     2221 catttagaag aaaacatctc aatatcaagg gattagtaca aaacctaggg tgggtgacgc
     2281 aataaaacca cggcgaggat tacaccaatc ttccccattg cctacataca tataagaaat
     2341 ttcctacttg caattaatcc tttgccgaca gaattttctt caattgcttt ttatgctctt
     2401 tctatcccaa ttaatagaag gagaaaatcg atgaaagcca tcaatccaga ccagcagtac
     2461 ctaagtaccc gacagagcgc caagatccta caggtatctc tggggacagt gcaaaaaatg
     2521 gtggaagaag gagatttggt cgcctggaaa acccgcggtg ggcaccgacg gatcttagca
     2581 acatcgctga atcagcagct aagacgtcgc aagctgggct tacaagaaag cgccagcaag
     2641 caacagtatc tagcgctcgg tatctttagg cgccccgaga acttagtgca aatgcgctcc
     2701 gcaattgaga gctggtccct aaggctagat ttagttggct ccatagacag cctagaaggg
     2761 ctgatgcaag cggtctcgct ctatcccgat gtgatttact tagattcctt aataccgccg
     2821 gttgagcagt tgcatctaat tcattacctc agcaagaaca tccaaaccag aagaattccc
     2881 atgctagtag acgaagcatt tgtgcagctc caccctggcg tgctcgatat ggcccaagaa
     2941 aatattcaac aactaaggcc tccggagttc gaggagccaa tcatcaacga agcactaaat
     3001 caacttccag agaaatctac tgtatttgca tactccgccg atagcgagca aggcaacgat
     3061 aagcacaatc caagattaga ggctctgttt gtagaagccc tcaataatgc cctctaaggt
     3121 ttatatccga ggcaacatgc ttagggcata cttgttttat aaaagaaaaa gccagttcga
     3181 tgaactggct tttctatcaa cgtaatgcga attatttaat caaaaacttg gtgcgatctt
     3241 tacctgcaat ccatttaggc gccttaccac gaccagtcca ggtctcacct gtcgatgggt
     3301 ttttatactt tgcagctacc ttaccccgct tcttgccaac gcgtttcttg ctagcgggtc
     3361 ctttctttgc tgggcgaccg cgcttacctt ttgctacacc tttgcgacca aatagatcac
     3421 ccacttctaa accaaattgc tcaatgattt ctttggcttt agcaattcct tcagcctttt
     3481 ctcgctgcat catggcatta atttggctgt ctaatttttc ccgctgtgct actaaatctt
     3541 taaacgatga cattatttat aaattccttt gaaaactaat gagtaaatag taatgattaa
     3601 tatacaaggt atattgatta ttcttttaaa taaaatatta ctagacctga ttatagatta
     3661 ataaaggtct ttagaagaaa gattattaac cctaaggcta tatattacaa ctaatattgt
     3721 aaattggtaa tttttatcat ttaaatcaat tagttaaata atttattaat aatataataa
     3781 tgctagtcaa taactgataa ttatattatt taatattaat taaattattt tcttactgtg
     3841 acttagcacg ccgtagcata aagctacccg tggatttggc ggttaattgc cctgcttcat
     3901 tgaaaatact accctcacaa taattaacgg tactgccagc ctttaatacc ttaccttcgg
     3961 caattaatat ccctttagag gggcgaagga agcttgtggt catatcaatg gtaatcacgc
     4021 ctaagatatg ttcgtgggag ctacgagcag ccgctgccat cgcaaaatcc aagatcgaca
     4081 tcacgacccc gccgtgggct acatggaaac tattgagcaa ctcaggccga agatgaagac
     4141 gaatacgaga atggccgttt tcggcaaact cgggctcgac ccccagaaca tcaaggaagg
     4201 gaatctctaa accaaaatac gttttactca ttatttggca aataatcgat caggatgggc
     4261 catggccttg aactcaattg cattacccga ggggtctaag aaaaacatcg tggcctgctc
     4321 accgacctca cccttaaaac gaatataagg ctcgatcaca aactgagtac ctgctttctt
     4381 taatttatcg gccatcgcct cccactcagg catcgacaat acgatcccaa aatgtcggac
     4441 aggtactttt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt caatccactc atcggagctt
     4561 cgtccctccg gacatccaag caggtctcca taaaaagctc ttgcgtcttt taagttatcg
     4621 actgggaatg ccaaatgaaa tgggtgcatg atgactcctt gatattgatg gtcggggcga
     4681 gaggatttga acctccgacc acttgcaccc catgcaagta cgctaccagg ctgcgctacg
     4741 ccccgacgaa aagataaatt gtagctcagc ggctaaggac ttgaagcacc tcgagcaact
     4801 cttcgcgcaa acgtaatgac gatttactct catcaagccg attttttgca ccgctgatcg
     4861 taaacccctc ttcgtacaat aaggttctta tttgtcggat taaaacaacc tcgtggtgtt
     4921 gataatatcg gcgattaccc cgtctttttt gagggcgcaa ctgatcaaac tcttgttccc
     4981 aataacgcag tacgtgcggt tttacaccac acaactctcc gacctcgcca atcgtaaaat
     5041 accgcttatt ggggatgggg ggcaaaagcg ggagactgtc cgcctttgaa tcaagctctg
     5101 ttttgattct cagcatggga ctcgactgcg tccttcagct tctgactagc gtgaaaagta
     5161 acgacgcgac gggctgcaat tgggatcatc tctccagttt ttggattgcg tccaggccgc
     5221 gctgttttat tgcgtaactg gaaattacca aagccagaga tctttacttc tacgcccgcc
     5281 tctagagtct gaccaatacg atcaaagaac gcatcgatca tatcttttgc ttcgcgttta
     5341 ttgaggccta cctgatcaaa taaagcttca gagagttcgt tcttcgtaac cgtttgattg
     5401 ttcgcagtca tatcgatact cttttcgtac ttgttgtctt aaatgaatga taaatataac
     5461 aaattactgg cctactagcg taaacgggct gcacagtctt tttgtaaagc acctaacaag
     5521 gcacttacaa ccgcttccac ctgagtgtct tgcaaagtct cttgatcatt gaccagggtg
     5581 agtctaaatg caaggctttt ctcgtcggca gccatagaag cgctctcctt ttgaggacga
     5641 aactcatcaa acaactcaat cttgtgcaaa aaaggttgtt tttttgtcag catggcatca
     5701 atcaggcttt gtgctggaac agactgcttg acgaccactg caatatcgcg atgcaccgcg
     5761 ggaaacttac taatctcatg aggccttggt aagccgatct ctgtcaccgc atcccaatgg
     5821 atctcaaaca agaccggtgc agatgctagc tcataggtct gttgcaaagc aggatgcaac
     5881 tcccctatcc aaccaattgc tgcattcgca aggtaaatct cggcggagcg accaggatnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncccttcaca tcaaagaaat
     6001 ccactagctg attattgctg gcccattgct cagggtttgc aaacccatag gcgaggccac
     6061 ctaattgttt attctgaata aaaccagcaa gcgctccggg ctgatcagtt tgcttagggt
     6121 cacgtttaaa tactcgaccg atctcaaaca aacgagcgcg gctagcacct cggttgagat
     6181 tgctgcgtaa gttatggagt aagccacccc ataaattact acgcatcacc ccatactggc
     6241 tggcaatggg atttaggact tcaatcggat tgggttgacc catttgagct tcggtttgac
     6301 gatcaatgaa cccaaagttg actacctctt gatatccctc tcccgccaaa cgatggcgga
     6361 cagcatggac aatgcgctta gactcgaggg gcgcactcat tttaagctct gcactgggtg
     6421 gcacatcggg gatattctca aagccgtata ggcgggctac ctcctcaatc aagtcctctt
     6481 cgatctcaag atcaaagcga tagctagggg gctgtactgt gaagaccgtt ttggagtcct
     6541 gtcccgtcac cgtgaactct agacctaaac gtttaaagag atcactaacg atcgccatgg
     6601 ttagcggtat tccgattact ttctcggcgc gagctaaacg catagtgacc ggtttacgct
     6661 ctggcaactg cttaatctga tcgtcgatcg gaccaagctg tccaccacaa acttgcacaa
     6721 tcaggtcact taaatactct aaggcagaaa tcgttccctg cgggtcaacg cctcgttcaa
     6781 agcgatgtcc tgcgtcggtt gtgaagttaa agcggcgcgc tcgtcctgca atcgcggctg
     6841 gccaccagaa cgcagcctcc acataaatat tttgagtagc atcaccaact gctgtgctgt
     6901 ctccacccat aatgccagcc aggctaatag ggccagactg atcggcaact acgccaactt
     6961 tcaatgcggc tgcagaagaa tcgggtgcag gttcaaccgt ttgctcattt aacaacttca
     7021 gactctcgcc tgctttaccc caacgaacca ctagtccacc ttgaatacga tcaagatcaa
     7081 atacatgatt gggttgacct gtctctagca tgacatagtt cgacaaatca accagaggcg
     7141 agatactccg ttgccccgca tgtgctaaac gctgcacgat ccactcagga gtcttaacct
     7201 tgacatttac acccttgata acacgaccag caaaacgccc acatagatcg gcatcttcaa
     7261 tcgtgaccgc caagcgatcc gcaatgctag ttattaaggt cttgcttttc ggaatacaca
     7321 gggcagcccc agtaattgca gcgacctcgc gggctaagcc aataacggat aagcaatcag
     7381 ctttattagg ggttagctta atcgtaaata tctggtcatc aagctttaaa tactcacgaa
     7441 tgttttgacc taagggtgca tcgacgggca actccagaat gccagcatga tcctctccta
     7501 aaccaagctc acggcccgag cacaacatgc cattactttc cacgccacgt aatttaccaa
     7561 ccttaatctg aaatggttta ccaccctctt ctgcgggtga taactcagca ccaacgagtg
     7621 cacatgggat cttgattcca gcacgtgcat tcggtgcgcc acacacaatt tgcaggttct
     7681 caaaaccagg tcctgcgttc actcgacaca cccgcaagcg atctgcatcg ggatgttgct
     7741 ctgctgagat aatttctgca acaacgactt tgctaaaagg tggagcaact gagcgttgct
     7801 cttccacctc taatccagcc atggtcattg catggcttaa agccttgcta tcgatcgcag
     7861 gatttacaaa ttgacgtaac caggactcag aaaattgcat atcagaacta agccggaaat
     7921 tgcgctaaga agcgcagatc gttatcaaag aagaggcgta aatcatcaat gccatagcgc
     7981 aacatcgtga ggcgctctaa accagaacca aaggcaaagc ccatatagcg ctctggatca
     8041 atccccatat tacgcaagac attagggtga acctgtcctg caccggagat ctctaaccag
     8101 cgtcctgcca acttaccgct accaaaggcc atatcaattt cagcagaagg ttctgtgaat
     8161 gggaaatacg atggtctaaa gcgtacctgc agttcattgg tctcaaagaa ggtacgcaag
     8221 aagtcggtat acacaccttt taaatcagca aacgagacac tctcggcaat ccacaggccc
     8281 tctacttgat gaaacatcgg tgagtgcgtg gcatcgctat cgactcgata ggtgcgaccc
     8341 ggtgcaatta ccttaatcgg cggcatctta tcttggccat accgtttgac atgctcactg
     8401 gcataccgta cctgaattgg actggtatgg gttctcaaaa gtaatggctt accttgagta
     8461 tccttaccat cgacataaaa agtatcttgc attgagcgag cgggatggtt ctcagggcta
     8521 tttagggctg tgaagttgaa ccaatcattc tcgatctcag ggccatcagc tacatcaaaa
     8581 ccaatcgaat gaaaaatagt ttccacgcgc tcccaggtgc gcatgacagg atgcaagcta
     8641 ccgacggctt gccctcttcc aggcaaagag acatcgatcg cctctgctgc taggcgggct
     8701 tgtaatacgt gatcagcgag ggcttgacga cgcgcattga gggcatcctc aacagcggtc
     8761 ttagcttggt taatatgggc accggcagtc ttacgctcat caggcgacat accaccgagc
     8821 gcttttaagc gctcagtgag taagcctgat ttaccgagat accgagcttt tgcatcctcc
     8881 aaagcagcag ggtcgctggc tttggagaaa tcgcgtttgg catcctcgac aatgtggtcg
     8941 agagaaacca tggctatgaa gacttaggct tgtactacag cagatttaac acgtgttacc
     9001 aaagcagcga acgcttcttt gtcaaacaca gccatatcgg acagtacttt gcgatcgagt
     9061 tcgatcgaag ccttcttcat gccattcatg aacacgctat aggtcatgtc atgttcacgc
     9121 actgcggcgt tgatacgggc aatccataaa gcacggaata cacgcttctt gttacgacga
     9181 tcgcgatagg catattgccc agcgcgcatg accgcttctt tagcgatacg gaatacattc
     9241 ttacgacgtc cacgataacc ggttgcggca tcggtgactt tcttatgacg ggctcttgct
     9301 gtaaccccac gttttactct tggcatagtg ttctcctaag atttaagcgt aaggcatcat
     9361 ggagcgaatc gacttaacgt cggctttcgc aacaggggcg gaaccacgta attgacgctt
     9421 gctcttggtg gttttcttgg tgaggatgtg acgcttgaac gcctgccctc gtttgattga
     9481 tccacttgcg cggaccttga agcgcttcgc tgcgccactt ttggtcttca ttttgggcat
     9541 gactgctccc ttttctttct ctgtgcgact gcggtggcaa ccgacggttg ctcttcctgt
     9601 accgatagtt cgctactaag tactacctac tgcaactaca aacctttact tctttttggc
     9661 gggcgctaag accatcacca tttgtcggcc ttccatcttc ggaaactgct cgacttgacc
     9721 ataaggctct aaatcaatct tgagccggtc taacatcttg actccgattt cttggtgagc
     9781 catttctcga ccccgaaacc gcagcgtaat ttttgttttg tcgccgtctt ccaaaaagcg
     9841 gatcaaatta cgtagcttga caccgtagtc gccatcatcg gttccaggcc gaaacttcac
     9901 ttcctttacc tggatcacct tttgcttaag ctttgcttca tgcaaacgct tggcttcttg
     9961 gtatttgaac ttaccaaagt ccatgatgcg gactacgggt ggttcggcat tgggtgcaat
    10021 ctcgactaag tcggtatctt cttcttctgc caaccgtaag gcttcgttca acttaactac
    10081 gccgacagca ctgccgtcgg atcctatcaa acgcacttca ggagcagtaa tttcctggtt
    10141 aatgcgttgc aatttttcag tagcgatctt ctaattcctc tctaacaaac aataaaaagc
    10201 caccacaccc cctagctagg ctcgggtccg actttctggg atatatcctg ctggagtcgg
    10261 gtgacaaaag cgtctagagg cattaccccc aaatcaactc cgccacgggc acgaacggcc
    10321 acagtattac tttcacgctc tttatcgcca acaactagca aataaggcag tttttgtaat
    10381 gcgtgctccc gtattttata cgtaattttc tcgtttcgca aatccgattc cactctaaac
    10441 ccttgttttt tcagcaattg ctggactttt tgagcatatt cggctgaatt ttctgagata
    10501 ttaagaacaa tcgcctgggt tggggccagc caaactggca tggcgccagc atgattttca
    10561 atcaaaatcc cgataaaacg ctctaaggaa cccacaatgg ctcggtgcag catcactggc
    10621 actttgcggc tattgtcctc agctacatac tcggctccta agcgggcagg catcgagaaa
    10681 tcaacttgca tagtgccgca ctgccaagag cgaccaatcg agtctttaag gtgatattca
    10741 atcttagggc catagaaagc accctcaccc ggcaactctt cccacgtttg tccggaagct
    10801 tttagagcac cgcgcaaggc ggcctcggct ttatcccaaa tcgcatcatc accgactcgc
    10861 ttagcaggac gcaaggctaa cttcactgcc acttccttaa agccaaagtc ggcataaaca
    10921 gctcgcactg ctttatcaaa agcagccacc tcggactgta tctgatcttc agtacaaaaa
    10981 atatggccat catcttgggt aaagccacgc acgcgcatca aaccatgcaa agcgcccgat
    11041 ggctcattgc gatggcattg gccaaactcg ccaaagcgca atggcaactc acgataacta
    11101 tgtaaacctg aattaaagat ctgcacatgg ccagggcagt tcattggctt taatgcatag
    11161 gcccgattct cagactcggt cgtaaacata ttctctttgt agttatccca gtgacccgat
    11221 ttctcccaca ggccacgatc taatatttgc ggagccttca cctcgtgata gccctcattt
    11281 tgatagacgc gacgcatgta ttgctcaact tcttgccaaa ttgcccaacc cttggggtgc
    11341 cagaacacca aaccaggcgc ctcatcttgg aaatggaaaa ggtctaactg tttagcaagg
    11401 cgacgatgat cacgcttctc tgcttcctcc aacatatgta ggtaggcatc ttgatcttct
    11461 tttttggtcc aagccgtacc atagatacgt tgcaacatct cattcttact atcgccacgc
    11521 caatatgcac cagcgagctt catgagcttg aacaccttta acttacccgt ggacggaacg
    11581 tgaggaccgc ggcaaaggtc ggtgaacttt ccttcggtat atagcgatac atcttcaccc
    11641 tgcggaatgc ttgcaatgat ttctgctttg taaacctcgc cttgtttctt gaagaactca
    11701 accgcttgat cgcgcggcat tacttgccga acaaccggct catccttctt ggccaactcc
    11761 gccatctttt tttccagagc gatgaggtcc tcgggtgtga atgggcgatg gtatgagaag
    11821 tcgtaataga aaccgttatc gattacagga ccaatggtca cttgtgcttc tggaaataat
    11881 tctttgacgg catacgctaa taaatgcgcg gtggagtgcc gaattacttc taagccttct
    11941 ggatttttat cggtgatgat ggccaattgc acatcacgat caatcgtgtg actcagatcg
    12001 actaagcgac catcgaccga tcctgctaag gctgccttag ctagaccgct cccaatattt
    12061 tgggcaactt ctgcaatggt cactggcgca ggaaattcac gctttgatcc atccggaaga
    12121 gtaacaacca ccatacctgc tccatcaaaa cgactacgta taaataagaa tgcgcgttcg
    12181 tcccaaacta aatgagcaca acgcgcatac caattacatc tctttacttc tctatcgttg
    12241 gtaggctcga ttggactcga accaacgacc cccaccatgt caaggtggtg ctctaaccag
    12301 ctgagctacg agcctatata aaacaagagt ttatcaccgc cggcgtactt ccgtgactcc
    12361 ccgcaagctc tccaggccag tttgtatgag tcttaaggct tcggtgttct gtatctcaac
    12421 ggtcaattgg atctgcgcta agcctttgcg gaccgatttc ttgagatcaa tcacatgcac
    12481 accgatctta gaggtaatct caaagagctc gcgcatcagt tctgggcgat ctaatgcgct
    12541 aatagctaaa tctgctggga acacccgatc tttccctgca ccctgctctg ggtcgagctt
    12601 cgcactccag gctgtttgga tgacccgctc gggtgcgcgc tcgagtaagg ccctaaatgt
    12661 tttacaactc ctgcgatgaa tcgatacgcc acgtccctgc gtgacaaa
//
LOCUS       DDPQ01000059            6168 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5163,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000059 DDPQ01000000
VERSION     DDPQ01000059.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5163"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3584..3648
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3979..4083
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5274..5310
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1710 a         1390 c         1222 g         1637 t
ORIGIN      
        1 aatggcatta tgcaagccga tggatctatc aaggtagaac ctcgtatgga tgttgatcat
       61 gtaggtcagg cggtattgca tatggcaaag ctaccgcccg agagcaatgt cttatttatg
      121 accatcatgg ctagcaaaat gccttttgtg ggtcgcggct aatcaataaa attgataagc
      181 taaaccaagc tcaaaattac gctctgctcc ggggaatatt ccgtcggggc tacgtcctga
      241 gataaatttc ttatcaaaga ggttgcgaac aacaccaaat aacttgatat ctcttttagg
      301 actgtaaaca acgctccaat ttaatgtggt gaaggatgga attaggcctt ttgttccatc
      361 attgctctct tccacagtgt ttgcactatc cgcatactga ctggatacgt ggtatgcatt
      421 caagaaactt ttcaagccat ccttgctgta gttgagtcct agattacctg tcaacttagg
      481 ggtgtatgga atgcgatttc caatgggccc caaggcgcct cccttgaatt cagcgactgg
      541 aatataggtt gcatttgcga gcacactcca ggctgatgtg atggcatatc caagcgcgcc
      601 ctccgcgccc tgatgtaaag attgtccgcc attcgttttg ctgatacctc cagagagggc
      661 ttggttgacg atctgattac taaagtccat tcggaatgct gccccctcat acgtccattt
      721 ctgggcttga ccacgaaagc caaactcgat gttcatagat cgttctgcat ctaatttctg
      781 gtcaacacca tcgccactaa ttgccgcaga aatcatggcc ggagcaaatc ctttataaaa
      841 actggagtaa agctgtagct ctggtgccaa ctgccaagta gctccaatac caggaacaag
      901 ctcagtgtta tttgcctgcc cctgcacacc attcatttca tttttacggt tctggcgata
      961 agactctacg cgaacccctg gggtcacagc aaactgatct gtgaacaaaa accgattttg
     1021 taaataaagg gctactgcct tggcatcatt attttcatta ccagtgatta ctccagttcg
     1081 cgccctaggg cccgcatttg agcgctctgt tttattgctc atggtttctg aatgaaggcg
     1141 tacaccaatt tccgactcat tttgaatacc aaaggcttgg taattagtaa acaatcgaga
     1201 atcaattccc aacatatcaa aattacgatt gcgacctgtc acgcagtatg ctgtgcctcc
     1261 acaatccaca aaactagttc cctgcctagt tttcgaggca acatccctac gccagaaatt
     1321 acgctctaat tggctccaat acatcaaggt tttgagcttc gtacttgtat ctatttctaa
     1381 ctcatgattg atatcaaatg aagttcgatt acttaaaaat tgatcatcgg gtgctggatt
     1441 ttttgttggc gtatgaataa attcatcagg tcttaggccc acataagagg tattaatttc
     1501 attttcatag cgtgttaatt taagacctaa ccattggctt tgtccaatcg ccatacctcc
     1561 ttttaccaaa atatcattca ttcggtagcc attatttcga aaaccatttg cctccgatgt
     1621 aattaaatta attcctccac ccatactttt ttgttctgag gacgtacccg cttcgagctc
     1681 aagcaatcta tacccatgag atccaagctt tcccttcaca atcccatctt taaggggtgt
     1741 tttactctga tagttaatta ctcctccgat ggtattgggt ccatatcgga gtcctgtggc
     1801 gccctttaaa acctcgatac tagaaatacg ctctaccggt ggactgtagt agctggcatt
     1861 tgataaaaat aaactgggtt ggattggtgc cccatcttct aagatgagta cctttgtact
     1921 acgatttgga ctgaggccac gaatcccaat attggggatg gcgccatacc cctcttcatc
     1981 ccgtgcatta acaccaggta cagtttttaa ggcatcctga agagaggcgg gctggatcat
     2041 ttccatctct tctgggctaa tgatatcaat agccccgggt attgaagatg cccctccttc
     2101 ttgtcgatcg atcacattaa tactgggaag ttctatctct ttagccatca atggtgagtg
     2161 agcgtagccc atggcaccaa ttaataaaga gacaaaaaga cgatttttca tatacaagct
     2221 ccaatagttg aaataaaaat tggctggcta tttgcatatg aaaatgctgt ggctggctgt
     2281 tagtagaaat taaatttatt tggtcaagac aagctttcct aatttagtga tacgtaattg
     2341 ataaataaga ccctcgtgtt caatatgaag gcattttgct tgacccagca aaagaaagct
     2401 tgaaatctgc cgcactgcat taactggctc taaagaaaat ggattttcac ccttgcgacg
     2461 aaactctgct tgagactgat tcatcattcg ccagttaaac gaaagcgaat gggaattcga
     2521 tagcatcgta aggatgagcg ttgtatacga ttcttcaata actccttcca atgctttacc
     2581 gcatcgaggg ctgattgatc caagcggtga tagccagagc ttttatcaat agttactaca
     2641 ttacttctgc caccagaccc catgcaactt cttacccaaa catccccttg ctctcttaga
     2701 cgtttactta ataaggggta atgcggcttc ggaatatcgc ttatctcgtc catctgaata
     2761 tcaaattctc caaactcaat atttttagag cttcctaaat gaactgtttg agtttgtaac
     2821 tcctcgagag tcttaatgct tgttttctct gcaaaatctt tttcactcac cgatttactt
     2881 tcttgggtag ctttcgtttt atttgacggc gctctctctg agagcttaac ttttataagc
     2941 ctatacatag aaggctctgc atacttttct ttgtaatcaa ttagccaata tatcaatcca
     3001 caatgaatag taaaaacggt cagaaaagta agtatcagag atctatgcaa tctcatcgag
     3061 tcatttctga ttacttaaat ccaaccttat ccaagattcg ttgtgcagct acttgattac
     3121 gcccaatagc agccaccgag accttctcga cttgataagg tcctaaggct ttcagggctg
     3181 ggttatctac tttaactgag gccactgcag gccattcatt attgccatct gccaaataga
     3241 tctgcgcagc atcgctagcc aaatactcca aaaataggac agcggctttt gggttagggg
     3301 catttttggc taagccgcct ccagagatat tgatatgaac tccgccagca ttttggttgg
     3361 gccaaatgtg accaatctta gagatgaccg caatgtcctc tggttttgtg gagcgcatta
     3421 agcgagcaag atagtaagaa tttgttagag caacgccaca ctcccccgag gctactgctt
     3481 tgatttgatc agtatcgcct ccctttggtg cgcgagccat gttagccacc atgcccttgg
     3541 cccattgctc tgtagctacc gatccatcac gctcaatcaa tgcnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ggcacgggtt
     3661 gagaacccaa accaagtaat accatcggca tctgctttgg cccgtaaatt agctggaata
     3721 cgctgatcta atatcttcga ttgaataggt ctaaataagc cattgacttg ggcacgccaa
     3781 agtcgtgcag catcaaccaa taaaataaca tctgctgggc ttcttgagcc ctcactgttt
     3841 aaacgctcta acagggcatt gtcatcggcc tcaatgcgat taatcttaat tcctgttgac
     3901 ttcgtaaagt tagcgtacaa agcctcgtcc gtttggtaat gacgagcaga atatagattt
     3961 agctctttat ttggttctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnatcctat cacaaatgag aatgattatc aataatattg atggttcctg gggtttctag
     4141 cgccccttgg agatggctct tgaaagtaag ataactggta aaagtccaac taatacaatg
     4201 cttaaggctg gtagcgcaag ctcagaaagt cgctcgtcag cagccagctg ataggtggcg
     4261 actgccaagg tatcaaggtt aaagggccgc aatagcaagg tagcaggcaa ctctttcatc
     4321 acatcaacaa acacaaacag taaagctgtc agcacgctac ggcgcagtaa tggcaaatga
     4381 attctacgaa tcatttccca cggttttgct cccagaagtg cggccgttcc atccatcgca
     4441 ggagttattc gggttaatcc agtttcaata ctttgcaagc cagaagataa aaaacgaatt
     4501 agataggcat aaatcaaaac agcaacgctc gccgacataa accatgcaat atcaagcaat
     4561 cccatcaatg acaagattcc aacacccaat acagctccgg gtagcgcata gccaaccgtt
     4621 agaacgcgat tgaccccccg cagtattttg ctttgactca ggcgcgcagc ataagcaaga
     4681 aatatggcac aaatcactgc taggattgca gtgagcgcgg atagtcctaa agaattcttg
     4741 agccaagaaa aatagcgtgg atcgatactg aagctctcac tcatgagcag gtgaagcaat
     4801 acccacatgg gcaatataaa tgctgagaat aaagtgaccc cacaaaagga agatgcatag
     4861 aatgcatgtg atccctttaa gcgttttggt attggtggct gagtattggt attacctaag
     4921 gcataacgct gacgagaacg attatgttgt tcccaataaa atacgatgag cacaaaagcc
     4981 aacaacatga gcgataagtg cacagcagca attcggtccc caaaggaaag ccacgcttta
     5041 aagataccgg ttgcaaaagt ctgtaaacca aaatacgaga ccgccccaaa atctgccagg
     5101 acctccatca atgccaaagc aatgccggta aagatagcag gtcttgtcat cggtagcact
     5161 agacgaataa aggcctcaac actgctgtag cccaatgttt ctgctgcctc catcagacgc
     5221 gcacttcgat ctaagaatga agtacgcgcc actaagtaca catagggata taannnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaactatcac gcatcatggt ttgcaaaggt
     5341 cctgagaact gcaaaaagtc gacaaagaaa taagccatca cataggtggg cactgccaag
     5401 ggcagaataa gcgcccactc gaatactttc ttacctggaa attgatagtt tgcgactaac
     5461 caggcattac ccacccctag aataaagacg ccaatgccaa caccaaaact taacaaaact
     5521 gtcgtgacaa catagtcatt taagacatag ctccatagat gaaaaagcgt acttcctcct
     5581 atttctccta attcggcaga agataaaggc tgacctgttc caaaaaaaat gggtaatagc
     5641 aagccaatca tcggcaatgc caaaaacagg gaaaggagaa ttgcagtagc gcgcattaaa
     5701 tggagatctg tcggggtctt atctgctatt tgtacagtat ttgtgaattt aagcaattca
     5761 atgagaaaat tatagggatg aattccaatc caaccctaat ttctcttgaa aacatcgcca
     5821 ttcagtttcc acggcaagat ggttctggca tcctagaaat tgtgaagggg cttagcttta
     5881 gcctgcccca aggcgatatt gcctgcctac tgggcccctc tggctgtggt aaatctaccg
     5941 ttttgcgcgc tatttgtggn ntgtggcttt gaacccttgc aaataggtga gatcaagatt
     6001 catggcaaaa cagttagctc aaagaatatg cgcattcccc ctgccgatcg taaggttggc
     6061 atggtctttc aagactttgc cctttttcct cacctcaccg ttcttgaaaa tgtgatcttt
     6121 ggtttgcgtg agggctccat cagtgcacgt aaacaaacgg ggatgcaa
//
LOCUS       DDPQ01000060            2572 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_27309,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000060 DDPQ01000000
VERSION     DDPQ01000060.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_27309"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1563..1597
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          678 a          604 c          602 g          653 t
ORIGIN      
        1 aatgccctaa tccttgtgca tccggaatcc ccgcagcatg tagttgatca ggctgatatc
       61 gttggctcca cctccgccat gatcaaagca gtagttgaag gcaaggcaag cgaatatatc
      121 gttgctactg atcaaggtat cttgcaccgt atgcgccaac tagctcccgg taaaaaatta
      181 attgctgcac cgactgcggg tgaaagcgca acttgcaaga gctgcgccaa ctgtccttgg
      241 atggccatga atggcttaca aggaattttg gattgcttag aaacgcaaac cggtgaaatc
      301 ttggtcgatg agttcattcg ctcccaagct ctaggatgta ttgagagaat gctgaccttt
      361 accagtactc accccgagct tttggcaaaa gcacagcacg gctttattaa aaatattggc
      421 gctgcctagt attttttcat gtttgagcaa aacgaatccc ttcctcaagc catcgctcgc
      481 aatgtgagag atgccttagc agaggatctt ggtactggcg attggaccgc tcagttggtt
      541 ccagaaaatc ggcaagccca tgctaaatta attgttcgac aaaaagctgt cttatgcggt
      601 attgcttggt ttgaggcgat agttcatcaa ctagatccac aagctcggat tcaatggaag
      661 ttccaggaag gcgagtggat gcaagcaaac tctaccgttt gcgagatcga tgcaaatgcc
      721 cgggcattgc tcagtgctga gcgccccgcg atgaacttcc tgcaaacctt atcctataca
      781 gccaccatta cgcgacaaca tgttgatgcg atcgctggag taagcccgaa tccaaatggc
      841 tgtgcactat tggatacccg aaaaacgctc cccggtcttc gccaagcgca aaagtatgct
      901 gtccgagttg ggaatggtaa aaatcaacga atggcactct gggatggaat tctgattaaa
      961 gagaatcaca ttgctgctgc aggcagtatc acggctgctt taaaagctgc cgatgctctc
     1021 cagtccgggg ttgatgttca gatcgaagta gaaaatattg cagagttaca ggaagctctc
     1081 tctgctggag caaaaagtat tttgattgat aactttacct tggatcaaat gcgcgaggcg
     1141 gtcaaaatca acgccagtcg ggctttcatt gaggcttctg gagggatcac actggaccaa
     1201 ttacaagcaa ttgcggctac tggagttgac cgcatttcta ttggtaagct aacaaaagat
     1261 attgaagcga tcgattactc tatgcgcatc gacccactaa cttaagtttt ttgggcttga
     1321 atcggagtca gaattgtggg gtacgaaact ggccaggttt ttgggtaatc acggctgtaa
     1381 tgcagaccac ggctctcttt gcgcattagt gcagaacgca caatcaattc tgcacattcc
     1441 agaagattgc gtaactcgat cagatctcgt gtcaccctaa agtttgcata gtattcctgg
     1501 acctctgttc gaaggagctc aatgcgatga agcgccctct cgaggcgacg ggtagtacgc
     1561 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct aatcgtgggc atctcaatat
     1621 caacttgatc tgcaatatca tgcgcagctg ccttaccaat tacaacgcac tccaagagag
     1681 agtttgaggc tagacgattt gctccatgaa ggcccgtata ggttgcctca cccactgcat
     1741 acagtcccgt taggtctgtt ctgcccttaa gatctgtaac aacaccgcca caggtgtaat
     1801 gtgctgccgg gacaaccgga attggctctt gcgtaatatc aatccctaaa gagagacatc
     1861 gagaataaat catggggaag tgttctttaa tgaaggcttc gcccagatgc cttgcatcta
     1921 aatgcacata atccaagcca tgttttttca tttcaaagtc tatagcgcgg gccacaatat
     1981 cgcgcggggc gagctcatta cgctcgtcat gctctggcat aaagcgtttt ccattaggga
     2041 gctttaataa accaccctct ccacggattg cctcggtaat taagaaagtt cggtcactgg
     2101 ggtgatacag gcaagtggga tgaaactgaa tgaactccat attaccaatc cgacaaccag
     2161 cgcgccaggc catggcgatg ccgtccccag ttgcagtatc aggattgctg gtgtagcgat
     2221 aaactttgcc taaaccacca gtagctaaaa ccactgcttt ggccaagatg gtctcgacct
     2281 gattatttgc gatgtccaaa gcatatgcac catagcagcg attagatttg tattgaccag
     2341 tggtttgtaa atgtttattg gtaattagat caagggcaat ccagttctct aagatcttga
     2401 tattaggatg cgcttttgct ttctctaata agacatcatg aatggcttta cctgtcgcat
     2461 cagcagcatg tgcaattcgt cgatgggtat ggcccccttc gcgcgttaag tgcaatcctt
     2521 tgggaccaga aacatcgggt gtaaacggca caccctgatc caccagccaa tg
//
LOCUS       DDPQ01000061            4463 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_45116,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000061 DDPQ01000000
VERSION     DDPQ01000061.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_45116"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    643..653
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1311 a          997 c          954 g         1190 t
ORIGIN      
        1 accgcaattt gtttttcttg ttgctcaatt ttgatagccc gattaacggt ggtgagtcgc
       61 ccaaccgtaa aaccaactac caaaatgcca aaggcaaagg cggctagacg taaacgatat
      121 cgactctctg gagataactg ccagtaatag tcaagtgcgt tctttaactt gctagataac
      181 gcttcaagca actggttaaa gtatcgaata aggttgggat tgaagtgctg tttcacatga
      241 attcctaaaa gggctcacgg cccgctgaca ccattaagac tgaataggcc tgtaacaaat
      301 cactctctgc gatcgttaat tggctttgta atactcgtaa ttgattttga gctgtcaaaa
      361 gatcattaaa gccctgccca ttatttgaat attgcgtcaa ggcgactttg taggcagcat
      421 cagcctgcgg tagttggcta tcccgcaaga actgcattcg atttttggtc tgctcataca
      481 ctgtgtatga acttgagacc cccaaaataa tctgttgccg cgttgatata ttagtcgcct
      541 cgactgccgc ttgtttacgc accgcctgct cgacgccata tttttctttc ataaagaaat
      601 aaagagggat gacgatatcg aactcgagct gataaaacaa ggnnnnnnnn nnnttggcca
      661 aactcacccc cttgcgagct gcatcgagat ccagctgtga gctgcgcaac aaaggatggc
      721 ttgactctgc atagttctca agctcgatta aggtgggcac atttcgaatc gcttgctgta
      781 cgtttccgga taaagcgatc ttctcgcgag ggtctctccc aattaacaaa ttaatattgc
      841 gataggcaat ttgcagttgc ttctcaaggt taaattgttc tgcaatcgct gcattttggg
      901 ccacttgagt attcaagaac tctacgtatg cggcggcatt attggcatag cgtgcccgcg
      961 acacattctt aatcaactca aagcgagtaa tcatctcttt taaaaccagt aactgctttt
     1021 gtgctgataa ggcactgtag taaagcgtcg aaagctgtgc tcccaactgc aaataattag
     1081 attcgctctg ggctaataga gattctgctg aggtatccgc aatttcagca gcaagccccc
     1141 tctttcctgg gaactgaaaa ggttgagcaa tcgaaattga gttattgcca ctcatattgt
     1201 tattggccgc ccctaaggca agcggagaat ttgcgggcat tcctgaccaa acaagaccaa
     1261 gttgaggatt ggcgggtgcc gcgatttgcg ggaccgttgc cttagcagat aaataactct
     1321 cgcgcaaagc actcagttga ggattactaa tcttcagctc attccacaac tgccttaagt
     1381 ccatttcctt ggagctaggc tgagcatgac tagccccgat gcctgcataa agcagcacaa
     1441 agcaagaaag tagtcttaag aacaaatgca tagaccttaa gaaatgggcc taaaaggcac
     1501 tggataaaca attaacacaa tcaatccccc gagcaagggt taaggaagta gagaatgatc
     1561 tctacaaaaa aatgacctac ttaagaaaag taagggggtg ctcgtgcttg acctgaccag
     1621 atatggccag ctcggataaa gtcggaatga tacgaatata aatctaaggt aatagtcaga
     1681 aatgagctgc gatcgcctaa tgaaatagtg atgagctcga tatcatctgc gagcgtattg
     1741 gtgacgatta acgtttgggc aagatcttcg ttatgccaca agataatgag tggctcaaag
     1801 cgctctgtta ctacgagtgt ttgatcgaca agctgaattt cttcaaaatc atccaaatcc
     1861 tcttgaaagg aaggagtggc cgcctggacc gagcacccaa aaataatgga tgcgatcaca
     1921 gcacagtgga ctagtttttg cctaataaat acatgcatgg cttgattcta gctcaggaaa
     1981 gcctttaaga acggggtttt atggcaattt tatgttgcag ggcacaaaaa agccgcctgg
     2041 taggcggact tttaggaatc aggggtaact gattacttgc gtttgtttac tgagtcttta
     2101 aatgctttac ctgcagagaa cttagcagtc tttgaagctg caatcttaat tgcctctcca
     2161 gtttttggat tacgacctac gcgagcagaa cgctttccct ggctaaatgt tccaaaacca
     2221 actaactgaa cggagtcacc ttttgttatt gatttgataa tgttatcaat tgcagaattt
     2281 aaggcacgct ctgcacttgc ttttgaaagt tctgcttcat cagcaatttt ttctactagt
     2341 tcagctttat tcatattttt cctttaaaca aacaattaaa aatctgctac aaaaagagtt
     2401 gtcatgacag cccaaaaatc cgagtcatac cagcaatgat taccaattgc tataaggccg
     2461 caattaataa tgcagtaagt ttgctacatc actcttctga aaacaagggg ctaaccctaa
     2521 ggataatccc tcatttttac tgtactttta tcccaatctg agcctttgct ggcttgaatt
     2581 tgcgggtaat ttcataatct ggttctaaat taagcgcttt tacttgacaa ttggtccgcg
     2641 ccttacaagc atctttctgc caatcataac ggttgcaatc accagacttg caaaaataat
     2701 attgatcgaa gtaatcggtt cagagaccaa aatagcagag gcaagaagag tacaaaatgg
     2761 ttgtaataac tgcacttgac taacgcgtgc tattccacca attgccatac cgccatacca
     2821 aaagaagaat ccaataaaca ttgagaagat actcaagtat agaaagctgg accacgccac
     2881 gaggtctgcc tgtaagtaac ttggctcaaa cgtcagcata ctaaacaagg cattaattgg
     2941 tagcgcaatc accagggccc aagagatcac ctctttagca ttgatggtgc gcgataaatt
     3001 accaccaact gcatatgaaa tcccattagc tagacaagca attaagagta agaaatcggt
     3061 gtactgaatc cccccctgac tgttgataaa ggcatagatc acgactaagg agcagcccac
     3121 cagggcgctc acccaaaacc ctaaggatgg gcgctcttta aaaaggagca cccccgctac
     3181 cacggttgcc aagggcatga tgcccagaat gacccccgca tgcgaggcct caccctcggt
     3241 cattgctaaa ttaataaaga gtgggaagat aaataccacg ccaatggcca caatcacaaa
     3301 ttggcgtagc tcacgcacgc taggtaattt tgctttgata ctgaatagat agatcgccgc
     3361 caagacccct gcaagaacag cacgggcaaa tgcaataaag taaggattaa aactttggac
     3421 tgctattttg gtaaccggca aggttaggct aaagataaaa ataccaataa agccaatcag
     3481 cattcctctt gtttcagttt tcacgggggt aattggtctc tagaaaagaa aatggcccca
     3541 ctgcggagcc attcaagcat taggtttaat gatttatttt ctggactttg gtttgattgc
     3601 aaacgacaaa taactaaggg caactgctgc accgaatgaa aatagcaaaa cagctgtttt
     3661 agattgcgga gcgcttgctg aaaagattag aaacagtgcc acggtaatac taatcaccat
     3721 gccagcaaaa ccggcgattt tcatggcttt atcaagatta ctcattgagt catttagtaa
     3781 catatgggtc tcctttttgg ggcaacggac aataaccgtg attattgtat ccccctttgg
     3841 gatcgatgct cctttttcct taattccgct ctaggcatgc gtcttcaagt actgaactac
     3901 ccagggagca tcgctatcac ttgggaagat gggatagtgc actgcttcaa tcacaccttg
     3961 atttgcaatc agcgtaacgc gctttaaaag agtcatgcca gccgcttcaa aggttggtag
     4021 attcagagct ttctgaaacg catactgcac atcgctcacc actggaaaag gaaggtgaag
     4081 acggtctgcc atctcttttt gatactgggt cgtttgaaca ctcaatccaa tgacctctgc
     4141 acctagatca cctaactctt gataatgatc cctaaaagag cagctttgcg gtgtacatcc
     4201 ccttgcccca ggtatttgat cccagccctc aggtagtggc acattaggtt gacctgtcat
     4261 ggggtagcaa taaatcacca atttcttttt gatctcgcca agattgatca tttttccatt
     4321 ggtggcttgt agagcaacat cagggagcct cattccaatt aaatgacgag tagctccgtc
     4381 atcgactgga atggggagat cggcggggag ttcctgataa ttgcttgata tggtcattag
     4441 aacgctttct gattaagtac gaa
//
LOCUS       DDPQ01000062            7348 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_8979,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000062 DDPQ01000000
VERSION     DDPQ01000062.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_8979"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3451..3507
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3939..4026
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4707..4790
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1927 a         1626 c         1742 g         1824 t
ORIGIN      
        1 ttctcttttg ccgttttatc agctagctct gccgctgcaa agcgatagag ttgggcgtta
       61 gcgttataaa accctgcaat aaccgttcct aaattaagaa gccaaaagtg tttctcaagg
      121 gtcgcataag cgcacagtaa ggatgcaaag aacgcaacga tgagcgcaat ctgaaacgag
      181 tgtttacgac ccagttgctt ttgaattctg gccacgatgg tggttgccaa agcactacct
      241 actacatagc ccatcactgg caaggtggcc atccaagagg ctggcgccag cgcaaaaccc
      301 actagaccat taatggcaat aaaagtgaca ttattggtta aataaagacc ttgggctaag
      361 gtcaaaaacc acaagttttt attcatatgc aagacaggca aagctctacg ataatgcatc
      421 agcttatgcg ccgatatttc aatctcattc tgatgggggc tgtaattact ggtcttgtgg
      481 cctgcgctag ctattccgag catgaggatc atgcggaggt caaaaagctt gatccacttg
      541 ttccgcgcga agagccacaa aagttctcag tacaagagga tggtcgtcta cccaaagggt
      601 ggagttttta tcgagtcaca cccaataaaa agaacacggc ttatcgtctg cagcaatatc
      661 aaggccgcac agtcctaaaa gctgaatcca atcaatctgc ttccggattg gcagtcaaac
      721 tgaatccacg tccatctcag cacttatggc tcaaatggga atggaaggca ctatcacacc
      781 ttgatcaagc agataacctt gataaatatg ccgacgatgc gccacttcgt ttgatggtcg
      841 cgtttgatgg tgatcgctcg aaactatcac tgaaagacag aatggttgcc gagatggcgc
      901 aacttctgag tggccaagaa atgccctatg ccaccttaat gtatgtttgg tcccccaaag
      961 ggcagttgga gcaaatcaag aacaatcctt acaccgagcg cgtcaagctg atcgctgttg
     1021 atgctggtaa agagaaccta ggtcaatggc gcatgcatca acgcgatctc actgccgatt
     1081 acatcaaggc ctatggctac gcacctggca acttaatcgg gattgctttg atgactgaca
     1141 ccgatgccac gaaatcacag accaaggcgt attacggtga tattgaactc ctatacagaa
     1201 aataccgcta attagttcag tcaaacaaga tctttctgtg ggacttttgg aaaaatcagg
     1261 ccaatccagt tatgatagtt ggttaacccc catatatcgt aaggcttatt gaggagacag
     1321 aattgtcggt cagtattgaa tcagtgcaac aggcacttaa gtctgtaaaa gatcctaaca
     1381 cccagattga ctttgtcagt gctaagtcag cacgcaacat caaagtaaac gatggggatg
     1441 tatcgatgga tatcgtctta ggctatcccg ctaagagcca gatcgatctc atccgtaaat
     1501 tagtggtcac ggcaatccgt gagttacccg gcgtgaagaa cgtgagcgtc gccattacga
     1561 ccgacatcgt agcccactcg gtgcagcgcg gtgtcaaact gttaccgggc gtcaaaaata
     1621 ttattgcagt tgccagtggt aagggtggcg ttggtaagtc aaccacagca gtcaatcttg
     1681 cattagctct cgctgccgag ggtgcgcaag tcggtatttt ggatgcggat atttatggtc
     1741 caagccaacc catgatgctt ggtattacag ggcgcccaga atcattggct gagaacacta
     1801 ttgagccaat ggaaggtcat ggcttacaag ccagctcgat tggtttcttg attgaggcag
     1861 ataaccccat ggtatggcgt ggcccgatgg tgacctctgc cctagagcaa ttgctgcggc
     1921 aaacacgttg gcgcgatctg gattacctga ttgtggatat gccccctggg acgggtgata
     1981 ttcaattaac cttgtcacaa aaagttccgg tgactgggtc tgtgattgtg actaccccgc
     2041 aagatattgc actgctggat gcgcgcaaag gtctcaaaat gttcgagaag gttggcattc
     2101 ccatcatcgg cattattgag aatatgagca cctatgtttg tcccaaatgt agtcatgaag
     2161 aacatatttt tggtgagggc ggtggcaaga aaatgtgcca agactatggc gttgacttct
     2221 tgggcgcact gccattgaac ttatcgattc gggagcaggc agactctgga agacctactg
     2281 tcgtagcgga ccccgatggt gcaattagcg caatttacaa agggattgca cgacaagtag
     2341 cgattcgggt tgcgaacctg accaaagata tgagcagtaa gttccccagt atcgttgtgc
     2401 aaaagactta gtaatagaag ctcatcatgc gttttgccat cattattcgc aaacagaaga
     2461 ttgataatcc atggcagaac tatcgttgga aacccattga ggtcattcct gatatcggtc
     2521 agtttggtaa aagtctagag cttgccggta cagagcaaaa ccgtaaagtg gtgggccgct
     2581 ttctggagcg agatgatgag ggcgaatcct ggttgtttgc aggttttgac tatcatttct
     2641 tcgccgatga ggcagagggc tattttctaa atatcactgc acccgagcct tgttggtttg
     2701 tcatgtggcg tctggaagtg gattatcaag actacttgga accaaattca atggcatctg
     2761 tgattaatga agatggtgca ggcattgcag taccacatcg tttgatgctg agttataacg
     2821 aggcatctcg tttaattgat ggtggcgagt cggtcgatac ggcaccattg caagcagaga
     2881 tgaaatcata cttagcggaa tatgtcacag caaactacaa acctgaaccc aagaaacgtg
     2941 cccggcccgc ttcattcaaa ggtgcaaacc gtcctgcaga gccccaatga cccaatctaa
     3001 tttcttaaat cgatgggcgc gcaagaaggc gggcgtcaca gaggagtccg ttaaggatgg
     3061 gtcgcagcag actgcccccg gtcttgttga taaggccttg cagccagacc ccgcattaac
     3121 tcctgcaccc gatgatttaa cgaaaccatc agagacaccg caagcactca cattagaaga
     3181 tgttgaaaag attgatgtga aagccgctga tttctcagct tttatgcgag cagatgtgga
     3241 cccagtagtt cagcaggctg ccatgaaaaa aatgtttagt gacccgcact ttaatgtgat
     3301 ggatggcctt gatatttata ttgacgacta caccaagccc gatcccattc cgatggaaat
     3361 gctcaagcgc atggctcaat ccgatatgtt gggtattttt aagaccaccg atgagctgta
     3421 tcccgagttt aaggatgcca aaaaggattc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnccc aatgataatg tgaccgttaa tccaaaccct
     3541 cgccacgctc accggcggat cgatatagaa tctagcgatt ctgacccaaa cgaaatccaa
     3601 aaccagcaaa ctggagagag acaaattaag cctgattcca gtacctagta ctgaaatcct
     3661 tagttttctg attgaatgac taaaaaactt atttgtgact gtaataaaag tatgcccctt
     3721 gatactaagg agctgggcct tcctgtccat acggctttgt gccggcaaga ggtaggtcaa
     3781 tttctgaatg cgctcaatga gcctgaggcg attgttgttg cttgtactca ggagcgggcc
     3841 ttattctcag aactagctac gcaggccgag aagcctttga ttgcccccct aaagtttgtc
     3901 aatattcgtg aacttgcggg ctggacgcaa gaggcagann nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnngtga tcggccctgg caagaccgcc tttcaatggg caaataaatt aggtgacagc
     4081 ttgcaggtca gtgttttatg tactgagcct gggtcgttac caaccggacg ggactaccca
     4141 actttcactg gtaaggtgac tcagttagat ggatatctag gacagttcaa agccaagtgg
     4201 gatttaaaga acccgattga tccggagatg tgtacgcgtt gtggtgcgtg cgtctcagtt
     4261 tgtcctgaaa atgccatcga cgcatccttt caaatcgatc taaataaatg taagtcccat
     4321 cgggcatgcg tgaccgcgtg cgctagcatc ggcgcaatta attttgagcg tactgatcaa
     4381 gcgcgtgagg gtgagtttga tctcatccta gatttacaag aaatccccag cattcagatt
     4441 agtcaaaaac cacagggcta ttttgcaccc ggctcggacc ccttcgaaca atcaatggct
     4501 gctagtcaat tgatcggcat ggtcggagag tttgagaaac caaaatactt ctcatataac
     4561 gacaagatat gtgcgcatgg acgcaatggt caggtaggtt gctccgcttg tattgatgtt
     4621 tgttctacga aggcaattca gtctacgttc aaagatggcc aaggtaaggt cgaagttaat
     4681 cctaatttat gtatgggttg tggagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgctgctcca
     4801 agtttattga tacatagtca aaacgccggt accgctctct tagatcacct cggacgcgca
     4861 gcccgcttgc gcgccaagga aaccaccggc ctgccagcgt ttgttattcc attagcggtt
     4921 gagcacattg cctcgaccgg aattgattta tggtttggat ccttggccta tggctttggg
     4981 gaaatactac ttttgctaag cggtgacgag gaccctggct atcgattagc gcttactgaa
     5041 caggtagatc tggctaactc aatattagta gcgatgggtt acagcaaacg cattcagtgc
     5101 atcattgcaa actccagtga ggatattgcg ccggttagca aagtcatgtc tgaactacgc
     5161 cagcgtaagg ctcacaaatt acttgcgaac cctgcaagtt ttgcgctttc tttacaaaag
     5221 cgtgagacct tggaaactag tttggagcac cttttgcaat ttgcgcccca agccttgcct
     5281 gcagaagggg tgccattgcc ggctcattcg ccactgggcg gtttgatcgt caataaagat
     5341 gcttgcacat tgtgtatgtc ctgcgtgggc gcttgccccg agggtgcatt actcgacaat
     5401 cccgatgagc cacagctgtc ctttattgag aaacagtgcg tgcagtgcgg actgtgtgag
     5461 caaacctgcc cggagagtgc cattgcgctg agcccacgct tgcgctcgat tgagcaccgt
     5521 aaagaaaaag taaccctcaa taaaaccgaa cccttccatt gcgttagttg tggcaaggcc
     5581 tttggaaccc taaagatggt ggagttgatg ttgggccgca ttggttccca tcaagcattc
     5641 tctggagaag cactggatcg tttaaagatg tgtagcgatt gccgtgtcgt tgatatgatg
     5701 aaaaaagagt tgtgatcgaa cgaatgacta aaaacgaagc agaccaaaaa gccgaactga
     5761 gccaagaggg ccttgcagaa gatctagcac gcgctgatct atacggtctt ctagccagtt
     5821 tattttttca gccacccgat caaatcatgt tggatcagat tgtcgcctcg ggcaagcaag
     5881 aaggcggcca aactggtgag gcgccccttg aggatgtttg gatgaacctc gtgggggctg
     5941 caaaaaatag taaagcactt gactggaagg tcgagtacga caatagtttt ctaggggttg
     6001 gtaaacccaa cgtgtttttg tatggctcct tttacatggc aggccatctt catgaaaaac
     6061 cgcttctaga aattcggcga tctcttcaac aatttggtct tgaggcctcg gattcgatta
     6121 gcgagaccga agatcatatt gcagccttgt tcgaagtcat gcggtatctg attgccggag
     6181 aggatgtcga ggtttcaaac cttacaaatc aaagggtctt tttcaatgac catattcgtc
     6241 cttggttcga tgatttgtgc gatgcaattg acgcagatca agaaattcat ctctacaagt
     6301 cggtttctgc cctaacacga gagtttttgg cggttgaggg ccagagtttt gacatgattt
     6361 aggcaatttc tatcaccgtg attgcccctt tctttaaggg taagtcctag cctacaaata
     6421 catgcaaaac cttacacaaa tactgtaatt ccaatacact catttcattg caacgcagta
     6481 actatattcg tataaaggaa tatttatgga tagcaaaaaa actctaaccg ctgatcaagg
     6541 cactaagcca tcccgccgca aattttttat tggtgcgggt gctaccgttg gtgcagcagc
     6601 cgttgtgtcc caaaccccat tgggccaggc acttgtgcaa gaagtaggta ccgcagttgc
     6661 tggcagcaaa gacggttatc accttaccgc ccacattaag aagtattacc aaacaacgct
     6721 cgtttaattg agcgtttttt acatctgaac tttatttaat tacgcactct cgcaggagtc
     6781 tcacaatgag cttaatccgc aaatcaaact caacatcgag ccaagttcca tcccaattaa
     6841 ttggtagctt gtcacgcggt cttcgtgccg ctgtgccaac catggatcgt cgaaccttct
     6901 taaagcgttc cggtatcggc gtcggcgctg gtattgcagc aactcaattg aatatgttgc
     6961 aaaaggctaa tgccgctgat ccgaagacag caatgctcga tgggaagggc aagattgaag
     7021 ttaagcgtac agtttgctcg cactgctcag taggttgctc ctttgatgca actgttgaga
     7081 acggtgtttg ggttcgtcag gatccagtgt ttgagtcacc aatcaatatg ggcggcgcat
     7141 gtgccaaagg cgcagcgtta cgtgagcacg gtcatggcga ctatcgctta cgtaccccaa
     7201 tgaaattagt agatggcaag tatcaaaaga tttcttggga tcaggccctt gatgaaattt
     7261 ctgccaagat gaaagagctg cgctctaagt acacccctga ttcaattttc ttcattggtt
     7321 catcaaagta caacaacgag caagctta
//
LOCUS       DDPQ01000063            2844 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_41046,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000063 DDPQ01000000
VERSION     DDPQ01000063.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_41046"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1551..1670
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          804 a          625 c          619 g          676 t
ORIGIN      
        1 cgtttgcctt taagggcaat taataaagca ggaatgacct tttggctaat cttctcacca
       61 agttcggtat aacttaatgg cactggaacc ttgcgatcct cttcagacag tggtggcatt
      121 aaattgggct tgagttttgc caaaataatg acgtagccga tatataaacc ggccaacata
      181 atgccaggga agaatgcgcc tgcatacagt tgcacaaccg atacgccagc cgttgcgcca
      241 tacacaatca aaagaaccga gggtggaatc agaataccta agcaaccacc tgctgtaata
      301 cagcccgcgg agacgcgagt atcgtaacct gcttttaaca tggcggggaa tgctaataat
      361 cccattagtg tcaccacggc acccacaata ccggtagctg ttgcaaatac tgcgcaggta
      421 aaaatagtag caactgcaag tgagcctgga actcgaacga gggcaagatg taatgatttg
      481 aataatttct caatcaggtt ggcacgctca accaaatagc ccatgaacac aaacagggga
      541 atggaaatca agacatcatt ggtcatgact gcatacgcac gttgcaccat taaggaaagg
      601 gtgttgtcga gcgctaagct aggatcctga aagctatacg aaatgaaggt gaagatcatg
      661 cccataccca tcagggtgaa ggcggttggg aagcccaaca tgatggcaac gaccaccaag
      721 gagagcatca agagacccat atgaccgctc tgaatatttc cccaaggcat taggatgatg
      781 gtggctaata ccaccaaggc catgatggag aagccaaata atagttcctt acgcattttg
      841 cttattcctt atctttgcca atcatggctt tgagtgcatc aacgtctact tcttcaacgt
      901 cttgctcgcg tgaaggccac tcgccttctt tgatgcaaac aacacagcgt aagatctcaa
      961 caataccttg gaccaataaa aaaactcccg caatcgggat gatggtttta aatggataga
     1021 gtggaggacc atctgcagta attgaggagt gctccaaaat tctccaggaa tctgcagcat
     1081 agccataccc cgcatacgct aaggcaatga cgcctgggat aaagaaaacg atatacaaaa
     1141 tgaggtcaaa aatcgcttga gtacgaggct ttaagaagcc atacaaaata tcgccgcgca
     1201 catggccatt cttggccaat gtataggcgc cggccatcat aaacatcgtg ccgtacatca
     1261 tgatttgggc atcaaaaacc caagggtggg ggctattaag tacgtatcgg gagaagacct
     1321 cgtaaccaat aagcagggtt agagcaagcg ctagccacga gaagcagtga ccaacatagg
     1381 tcgatatttt atcgactgtt aaaaagaggt tttgcatagc tatacctttg attcttatat
     1441 gagaaatggg atggtaagta attaactaac catcccattg atttactgct atcagcattc
     1501 taacaagaaa tgctgaccta cctcaattta gcttaagcct ttttaggagc nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcatcaaata
     1681 ccttcaactg ggtccgcaat accgagtcag gggtcttgta gaacttgaca ccctgttttg
     1741 cttgcatctc agcataatct ttggagtagc gatcaactgc tttccatgac atttcagcag
     1801 aagctgcatc aacggcatta tctaatgttg cacggagttt cgcaggtaga gcgttgtact
     1861 tggtgccatt gaacagaatc tcaaactgct cagcattttg gtggaagctt tgtaacatgc
     1921 aaaccttcga tacgtctggg aagcccaaga gacgatcgga agacgcatta ttaaactcag
     1981 ctgcatccaa gagaccacga tccatcgcag gaataatctc gccgcctggc aatgcgttta
     2041 cggaaacgcc cattgcggta aacatatcaa tcgcgatacc cacggtacga tacttgaggc
     2101 cttttagatc ctctgccttg gtgattggct ttttgaacca accaagaggc tgcgatggca
     2161 ttgggccgta tacataagac ttcacgttgg cgccaactac tttatagagt tcctcaagga
     2221 acgcttgacc accaccgtac ttatgccatg aaaggagcat gttggcatcc ataccaaagg
     2281 aaggaccaga tccccataat gcgagagcag cgttcttacc gtagtgataa gcaaccacgc
     2341 catggccacc atctaatgtg cctttagaaa ctgcatcgag caatccgaat gctggaacta
     2401 cagcacctgc tggcaatact tcaattctga gttcgccgcc ggtcatgtcg tttacttttt
     2461 tagcaaaatc gagtgcgtac tcatggaaaa tatcctttgc gggccaagtg gactggaagc
     2521 ggaaagtttg tggtgccgct tgaccttggg aaatcattgg gaagctaaga gcagctgctc
     2581 cagcgctacc gactgctgca ccttttaaaa acttacgacg aggtgcttgt tttttaattt
     2641 cggacattca tatctcctgg ttaataacga ataagtgctg catgtaaagc agagggaaat
     2701 tgtaggattt ttaaagtaat agagatatcg ggtaaaccct ttgtttctat ggctatgtgg
     2761 ttatttatta cgccccattt gggggatcct caaaatcctc gtgatacgat tcatgagatc
     2821 ttggattgtt gcaatgcatc acca
//
LOCUS       DDPQ01000064            4271 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_27779,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000064 DDPQ01000000
VERSION     DDPQ01000064.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_27779"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    254..308
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1236 a          901 c          932 g         1143 t
ORIGIN      
        1 cctcagagca gggagcaaag gcgattgtgt tggcagcaaa accccttcat gaacccattg
       61 ttcaacacgg accgtttgtg atgaacaccc gtgaagagat tgagcaagcg attgatgact
      121 atcaacataa ccgattaaca gcgatataac taaatttcat gagcctttat caacagttac
      181 aagaacgcga acgtcatcag aatcctattc gagttggttt aatcggagcc ggtaaatttg
      241 gcgccatgta tctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnct aaatcgattg agatggcagt tcaggaaaaa agctgcttta ttacagacga
      361 ttggacaaaa ttagttaatg caccacagat tgatgtaatt gtggaatgta ctgggaaccc
      421 cattgctgca gtcgaacatt gtttaaaagc tttctcccaa ggtaaacatg tggtgaatgt
      481 caccgtagag gctgatgcat tttgtggacc gctattagca aaaaaggcca aagaggccaa
      541 tgtgatttat tccctagcat ttggtgatca gcctgcctta atttgtgatt tagtggattg
      601 ggcgcgtacc tgtggattcc cagtggtcgc agcagggcgt ggccataagt ggttgccgca
      661 ttatgctgat tccactccag atacagtatg gaatcattac ggtcttagcc ctgagcaagc
      721 ggtacgaggt ggattaaacc caaaaatgtt taatagcttt ttggatggct ctaaaccctc
      781 tatcgaatcg accgcggttg ccaatgcaac ggggctactc gtaccaagca atggtttgct
      841 ctaccctcct gctagcgttg aagatatccc agttgtcaca agacctatga gtgaaggcgg
      901 gcaccttgaa caaaagggaa tggtagaagt aatttcatcc cttgaaaaag atggcagaac
      961 aatcccttat gacattcgca tgggcatctg ggtcacagtt gaggctgaaa ccgaatacat
     1021 caaaaattgt tttgaagaat ataaagcaca tactgacccc agcgggcgtt actttacgct
     1081 ctataagcgt tggcacttaa ttggtctaga agtcggtatg tcggtagcta gcgttgctct
     1141 tcgaaaagaa gctactggag ttccttattg ctggaatgct gacgtggttg ctactgccaa
     1201 gcgcgatcta aatcctggag atgtattaga tggagagggt ggatatacgg tttggggcaa
     1261 actcttnncc agctaaaaaa tcatcacaaa tgggtggact acctttaggc ttggcgcatc
     1321 aagtgaaagt ggtacgacct gttaagaagg gtcaaagcct ttgttgggac gatgtcgcca
     1381 ttgataaaac aactgatgcc tatcgcgttc gtaaagaaat ggaagtcatg ttttgatggg
     1441 atcgacttcc tttgcgcctg ataaatagat ggctcaagaa aaatccaata aacaaaaagc
     1501 caaatacgta atgggccaat agggttaaat ctcgcaaagg ttcaggccca tagtagagta
     1561 gaaagccgct tcctattagg gtcaacaaac agacaatttg gaatacacca gaaaggcgat
     1621 tgctctttgc aagccagcca gctttgagat gaactggcaa cactaagcca agcgcaatca
     1681 atacaaagct agcagagatt ccgtgggtag ctaagataaa acgggatgcg ggaataaaaa
     1741 catatagttg taattgaggg aaaaagaaat agacgagacc cgatagggca cagagaaaga
     1801 acgaagataa gataaaactc ctttgccagg atggcatttt tcctaaacga ctcattttta
     1861 tcctttaatg actaatccaa tgctgttgta atgagaaaag cagggatgct tactatgtcc
     1921 cagagaagcc aataccttgg tcaagccatc tgcataaaca cattggggag cgatcaccgt
     1981 aaaagaagaa tctgtacgga tagtactttt tcgttgtgga ttaaataaat gcgcttggag
     2041 gtagttgcct gaactggcta tagaaacatt attcatgttg ccaattgcat tggcaagtag
     2101 gggattgatt gggttgcgaa tataaatcaa cgctggtgca tccccaaata agcgtagatc
     2161 gccacccgca ttcaccagac cattcgtaat accaaagttt tctagcacct caaccgcttt
     2221 atctaccgca tatcctttgg caatcccatt gagattgatc gatatagggc tcttgaaatg
     2281 aatatgctcc ttatccaacc actcaacatc atcaaaggaa tgcgagcaat ggtctagttt
     2341 ggggtcgtag gtgcaatcaa ataatccatt cgattgccga tataaatcac tcgctatcag
     2401 taaaacctct tgaatacagg ggtgtaattt tgttattcct aagggggcta atcgatttat
     2461 cttcgaaagc tcgctatctt tctggtgaat attcatgagc ttatcaacca tagcgatttc
     2521 ttgaaaagcc tcatttattg cctcaagagc aagtgattta aatgccccat cttgattcct
     2581 aaccgaaatc tcaacatagg tgcctaaaaa aactttgcat cgagtcatca ttcggatttc
     2641 agcgcaatgt catataaggc catgactcgc ttcacaccat cggtcacgtg tttcgcagat
     2701 agggtagcgc ccgagatatt ttgtatatct tgattgagct taattggatg actggcagtt
     2761 ttaccaataa actgtcttct ccaatcatct ccttggattt catagccata agattccaca
     2821 tattccataa tttcaatacc aaggatctct cctttggaat taatgccaac ggcataggtg
     2881 atcatttcat gcttacctaa tacttcatca acgattagcc atgatccatc tgctgtcttc
     2941 cagattttgt ctcccttaaa gggatgccgg acgcttgaga ttgagcgcat ctttttttgt
     3001 aaatcatcgc tgatgatgat ggggctcttt ttcaatactt ccttaggaaa gaacaaactt
     3061 ttcacttgct ttatagaaag atagatacgg gcctcaacga tcgctggcgc actgatcgca
     3121 atgcatccaa taagcattgc gatactggat tgggcaggat tcatgtcaat tgagggggat
     3181 accaaactta aacatgaact cgttcagtga gtggctgtcc catactcgac cgtttgagca
     3241 ttcggcctca ccaccaccca tgcagttacc ccctttgagt tgatagcgcc atgcgatagt
     3301 tgcccaccaa tccttggtgg catattgcag atttggtcca acaaaagtgg cgcgttgaat
     3361 ttggttacgc aggttcagtt ctgagtagtc attatgaaag cgggcctcga cacctgcacg
     3421 ccatttgggg gcaaagcgat agctggcacc aactaagaaa tccaacatgg actctggaac
     3481 attgccgtta tcgataaatt taagtcgttc attagcaacg acaacgttac cagctagaac
     3541 caattgatca tctaggaaat tagattgcaa tagcagtcgt gcggctaact catttttgtt
     3601 tttcccaatc gtcggctcaa agtaaacccc tacgcctatg ggtgaagtca ctggattagt
     3661 aaagcgatat atggcnntct aatgaggcac cttcgatccc agacttgcga taggcgcttg
     3721 caggatcatg cgaggaggga acgggatagc cgcccgtgca ggaaggctca ccctcacagg
     3781 catcctgatt ggtgtaattt tggtttgcgc tgacgtaata cgaatttaga tatgccgcta
     3841 cctgcaaatc attagtaagc ccatactcaa tttctgatct tgaaaaccaa gagtcatagg
     3901 tgcctgctgc ctgttgctta ttgagttgca gtcgttgctc aaattcccat ttcccttttg
     3961 gctgcagatc gagggtgtag atccagccaa ataaaccatc tccagccaaa actggctgtg
     4021 agaatagcgc catcaacatg ccaattgaaa caatcccttt cttaaatgat cctgaataag
     4081 tcattcctac accctttctg attaatgaat agagactcaa ttctaaatga gaatcattat
     4141 cattaacgaa taaaggggct tatagtattt ctatagacaa caaaaaaccc cttacgtttt
     4201 tattccataa ggggtttatt gttaaagcta gacagcacca ttatcaataa tggtggagtc
     4261 ggcgggaatc g
//
LOCUS       DDPQ01000065            8680 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_8788,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000065 DDPQ01000000
VERSION     DDPQ01000065.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_8788"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1085..1145
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4783..4867
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5134..5231
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6804..6837
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2179 a         2182 c         1935 g         2106 t
ORIGIN      
        1 acgttgtaca aaataactgg gagatctact ttctcagcga tagtcttaaa gtgcgcatac
       61 atgccatctt gtgttggctt gttgtaatac ggtaccactt gcaaactggc gtttgctccc
      121 acctttttgg caaactcggt taactcaatg gcctcgcgtg tggaatttcc accagtaccg
      181 gcaatcactg atatacgacc tttaatctgc tctacggcta cgcggattaa ctcgcaatgc
      241 tcctcaactg ataccgtggg tgactcgcca ctagtaccca caatcacaat cgcatcagta
      301 ccttctgcaa catgccaatc taatagcgaa cgcaaggcag gatagtctag gctgccgtct
      361 tcatgcatcg gggttgcaat cgcaaccata ctaccggtca tcaattgggt catggggttt
      421 tagagaatta atctactggg gttcttaagc ttgaaattgt aacggatggc gtattttagg
      481 ggctccacgg cacgcacaaa ggctttgaac catggctggt ttgggtcttt ctacaaaacc
      541 gtctcgatag gcgaccacat ggagttgatg tttttgggct aactccaata actcaccatg
      601 acgcagtaaa aatgctgggt tcgaaggttt accaaactct gcattgccag tggcaaaggt
      661 ctcgtacagc aagatgccgc cctcttctaa aagggttggc aatagatcca aataagggcg
      721 ataaaggtag ttcgtaacaa taatggcgtc ccagcgccca aactctgctt cagggaggac
      781 ccaatgatca ccctctaggt caagtgctag ggttttaacc aagcgatgct tgatcttggc
      841 taaagagtct ggatcacgat caacagcaag aactgtatgg ccttgatcag ccaagtaacg
      901 tgcatgacgc ccgccaccac atgctagatc taagacctta gcaccaggtt ttagtagcct
      961 agcatagtga gcaatccaag aagagactac atcaagctct gcgtgcactg cgggttcggt
     1021 attaatcaaa gtcgaggccc atggcctcac gcacatcgcg catggtctct tgcgctactt
     1081 tgcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnncgctc aaacatgggt tgctgttcac gtaagatcgc atcaatcact ggttgcttgc
     1201 aatctaagca gccaatacct gcagatttac aacccttctc tgcccaatct ttagtggctt
     1261 ggtctgagta cacctgatgg aactgccata aaggacagtt tgcgggattg cctgggtcgg
     1321 ttcgtcgcac tcgggctgga tcggtaggca tggtacgcac caaacgaata acctcctcag
     1381 gcgcctcgcg gatactgatg gtgttgccat acgacttcga catcttctgg ccatcgagtc
     1441 cgggcatgcg cgatgctgat gtaagcaagg cttggggctc taccaaaata attttgcggg
     1501 cgccctctaa gtaaccaaag agacgctccc gatctcccat cgacaaactc tgtgactctt
     1561 gcaacatcgc ttgcgcttgc aataaagcct catcctcacc acgctcttga taagcggtgc
     1621 gtagttctgc ataaagtttg gcgcgcttac tacctaattt tttaactgcc tctaaggcct
     1681 tctcctcaaa gccgggttca cgaccataca aataattaaa gcgtcgggct acttcgcggg
     1741 tcatctccac gtggggtact tgatcctcgc ccacggggac atgcaaagca cgatagatca
     1801 aaatatcggc tgcctgcagt aatgggtatc ccaagaatcc gtaggtttgc agatctttct
     1861 ctttgagttt ctcgatttgg tctttatagg tgggcactct ctcgagccat cctaatgggg
     1921 tacccatcgc taacaacaag aacagttctg aatgctcagg cactcgactc tgtataaata
     1981 aagtggcttg attaggatcg actccagctg ccaaccaatc gatcaccatc tcccaaaccg
     2041 actcctgaat aacctcaggg gtctcatagt gagtagttaa ggcatgccaa tccgctacaa
     2101 agaagaaaca ggggtactca gcttggagac gaacccaatt ttttaagacc ccgtggtaat
     2161 ggcccagatg caaggcaccc gttgggcgca tgccagaaag aacgcgttca gcaaacatag
     2221 gaaggattgt cgcttattta atgaaagacc gaccagatcg gctttagatg ctctggtaga
     2281 tgcggtagta agcctagatc gatattactc tcgtgcggat catgaaaatc tgaaccgcgc
     2341 gaggccataa atccgtaacg ctcggcaatc ttggcatagg ttttgtattg atccgggcta
     2401 tgacttccgg taacgacttc aattccactg ccacccaagt ctttaaagcg agcataaagc
     2461 tcatccatct gcatcgtatt gagtttgtat cgtccaggat gcgcgatcac tgcctcgccg
     2521 cccgcccccc gaatccagct cactgcctca tcgagactag cccagcgatg cgatacataa
     2581 cctggcttac cagcgatcaa gtatttacgg aacacctcat ccatattgct gcatattttt
     2641 tgttctacca agaatctggc aaagtgcgta cgggcaatca actctgggtt gccagcaaaa
     2701 tgcagagcgc cttcgtaact attttggatg ccaacttgct cgagttgcgc agccatttga
     2761 cgtgcacgta gaccacgacc atcccgagtc gcacgcaagc cctcttccag aacagcattg
     2821 gattgatcga gaccaaggcc cacaatatgg accgtttggt tcatccaact aatcgagatc
     2881 tcaaccccgg cgagataggt catgccaagg ttttcagcgg caagacgcgc gcgctcttgc
     2941 ccacccaaaa catcatgatc ggtcagcgac caaacctgaa ccccattttg gtgagcacga
     3001 agggctaact cttcaggact taaggttcca tccgaaataa tcgagtggca atgcaaatcg
     3061 gcattcagtg gcgcagtttg gctcatacct ctattttagg tcagatgagc attaatgatc
     3121 cgacgggggg attcaatata gtctcgggat tgcatctcga tgaggcgtga gaccgttcgc
     3181 gcaaactcat ccaccacctg tccctcggta taaagctggt ctggaggtac ttccgcagac
     3241 ataattaatt taacccggtg atcatataaa acatcaatga gccaaataaa gcgtcttgct
     3301 tcgttagtta gccggggtgg catgtggggt acccccgcta cgattactgt atgaaaggta
     3361 tttgcaatct ctaggtaatc attttgagag cgaggcccac aacagatggt ctggaagtca
     3421 aaccacacta ccccttctgc tagatgcaag gcccgaattt ctctagactc aatctttagt
     3481 accggatacg aagactctat ttgcttacca atcagattat ggaagtagtc gtctaacttt
     3541 ttatcattct cagcggttag tggatgcaga taggcttgga tacgcgacat ttgcagttgg
     3601 cggtaatcac tcccagcgtc gacattcaat acatctaact tatcttcaag cagttcaatg
     3661 gcaggcaata agcggtcacg gtgcaaacca tttggataga gttgatctgg acgatagttt
     3721 gaggtcatga tgaactggac tcgatcaatg aacaatgctt tgagcaagcg gtacatgatc
     3781 atcgcatcgg caatatcgtt gatatgaaac tcatcaaagc aaattaagcg atagcgctct
     3841 gcaattcgca aggcaagctc atcaagtggg tctgccaagc ctgagagctc atgtaactcg
     3901 cgatggacct cgcgcatgaa ctcatgaaaa tggatgcgga tcttcttcgt aacaggtgat
     3961 gcctcataaa agcaatccat taagaaggat ttacccctgc caactccgcc ccaaagataa
     4021 acccctcggg ggagctctgg atgaaatatt tttttggtca gcgcattgct gcggatctct
     4081 ttatacgcca cccactgatc ttcgcactgc tgtaaacgca caatggcctg ctcttgcgca
     4141 ggatccggtt gatagccgcg cgctttgcac tcttgatgat aaaagtcagc gactttcaag
     4201 ttacatattg agtgcgcgtt gatccgttgc taaagcagct tcacgcatca cctcagatag
     4261 gctggggtgg ggatgacaaa cgcgagcaat atcttcagcg gctgctttga actccatcgc
     4321 caatgctgct tcggcaatca gatccgatgc attggggcca ataatatgga ctcctaaaac
     4381 ttcatccgtt ttggcatcgg ccaatatctt cacaaagccc tcagctcggt ccatgccaag
     4441 cgcacggcca ttggccatga agggaaattg accaacttta taagcgcgtc cctcagcttt
     4501 taattgttgc tctgacttac caacccaagc gatttctggg tcggtataga taacccaagg
     4561 aatgcagttg taatcaatat ggggtttttg tcctgccagt aactctgcaa ccaatacacc
     4621 ctcatcctcg gccttgtgcg cgagcattgg tccgcgcacc acatcaccga tggcatatac
     4681 acctggggca gcggtggcac aggtatggtc attgatgggg ataaagccgc gctcatcggt
     4741 ctttaactgc acagcctgaa gattgagacc ttctgtatta ggnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnacc ttatcaatct tcacacccat atgcatatcg agtccttgtt tggtgaagat
     4921 cttcttagcc tctttagcaa tgccctcatc gcaggcaccc aagaaggtgg gcaaagcctc
     4981 taatactgta acttgtgaac cgacacgacg ccataccgag ccaagctcca atccgattac
     5041 accggcacca atcacaccca agcgcttggg taaagaatca aaattgaggg caccttcgtt
     5101 atcgcagatc agaacgttat ccactgtgat accnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn naataagaaa ttaatcccgc cggtcatctt ggtcacgatc tcgtctttac
     5281 gagcaagcat ctttgcaata tcgatactga ccttacccac cgtgatgcca tgattggcaa
     5341 cgtgatggcc gatcttctca aactcttcgg acgaggctaa taaagctttt gagggaatac
     5401 aaccaacgtt taggcaagtt ccacccaagc gaggctcccc tttgggatct gcatatgggt
     5461 tcgcttctgc acaggctact ttaaatccca gttgggcagc acggattgca gcgatgtaac
     5521 cacccgggcc agctccaatg accagcacat caaatacttg gctcatggtc tactccttat
     5581 aggtcaagaa gtaaacgggc tggatcttct aagagttcct tcatcgccac caagcctaag
     5641 actgcttcgc gaccatcgat gatgcgatga tcgtaggaca gtgccaaata attaatcggc
     5701 cgaattacga tctgtccgtc ttcaaccacc gccctctcct tggtcgcatg aatccccaaa
     5761 atagcggatt gtggtggatt gatgatcgga gttgaaagca tcgaaccaaa gacgccgcca
     5821 ttagagatcg agaacgtacc gccagttaac tcttcaatgc ttaatttgcc atcgcgggct
     5881 ttattaccgt aatcagcaat ttgcttttca atttcggcca aagtcagttg atccacatta
     5941 cgcaagatgg gaaccaccaa accgcgtggc gagcttacgg caataccgat atcaaagtaa
     6001 ccgtgataaa cgatgtcatt accatcgacc gatgcattga ggatgggata ctttttaaga
     6061 gcatacgtag ccgccttcac aaagaaggac ataaagccta atttaacgcc gtgggttttc
     6121 tcgaaggtat ctttataacg agtgcgcata gcaatcaccg gcgccatatt gacctcattg
     6181 aaggtggtca agatagcgtt attagcctgt gactctaata agcgctcagc aatacgagcc
     6241 cgcagacggg tcattggaac gcgctcttca gtacgctcac ctagagataa attagagatg
     6301 ggcaaaggtg cggctgacga cactgtcttc gctccaccag aaacagtatt taggacatcg
     6361 cctttggtaa cacgtccatc tcgacccgtt cccgagactt gcgcagcatt gagattgttc
     6421 tcggccatta acttggctgc tgaaggcgcg gcaattgcgc cttttactgc cgcaggggct
     6481 gaggctggtg ctgctgcttt aggagctgca gcgacagtag ctttaccttc actatcaatt
     6541 tttgcgatca cttgctcggc tactacggtg ccgccgtcag caacgataat ctccgatagc
     6601 acgccggcag atggcgctgg tacctctagc actactttgt cggtttcgat ctcaatcaag
     6661 atctcgtctt gagcaacagc ttcgccgggt ttctttttcc attgcaatag agttgcttca
     6721 gcaacagact ctgatagttg gggaactttg acttcaaata aggccatgat gtgtcctatg
     6781 aataggttaa ataatgagtg gtannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt
     6841 tgctgagtct ggtgcaaatg ggcatagccg actgctggtg atgcagaagc tggtcggccc
     6901 gcatatccca aacgcattcc ttcgctcatg ttctcaagca agttatgttg cacaaagaac
     6961 catgcaccct ggttttgcgg ttcatcctga caccaaacca gttccgttac attgggatac
     7021 gttttaataa cgtttgcaac tgctttatgc gggaatggat ataactgctc aatccgcaca
     7081 atcgccgcat tctctaattt cttatcagtg cgttgcttaa caagatcgta gtaaaccttg
     7141 ccagagcata tgatcacacg ctcaatcgac tgaggatcaa tcgacgcatc actctccgga
     7201 atcacagttt gaaactcacc cttcgtgaac tcgatgagtg gcgatgctgc atctttatga
     7261 cgcaacagcg atttaggagt catgataatg agcggctttc taaattgccg aatcatctgc
     7321 cgccttaaga gatggaagat ctgcgacgcg gtcgtgggtt gaaccacttg catattggta
     7381 tcggcgcaca actgcataaa gcgctcgagg cgggccgagg aatgttctgg accctgacct
     7441 tcataaccat ggggcagcat catcaccaaa ccgttgacac gaccccattt cacttcacca
     7501 gaggcgatga actgatcgat gaccacttga gcgccattaa cgaagtcacc aaactgggct
     7561 tcccaaatcg tgagtgtatt gggctcagca gctgcataac catattcaaa tccaagaaca
     7621 gcctcttcgg aaagaatcga gtcaatcacc gtaaagggtg cttgatcttt gctaatgttt
     7681 tgcaagggaa tgaagctgga atctgaattg ccacgctctt gatcatgcaa taccgagtgg
     7741 cgatgactga aggtacctcg gccactatct tcacccgata aacgaatggg gtaaccactg
     7801 gcaagcaagg atgcgaaggc catcgcctcc cccatccccc aatcaacatt gatctcacca
     7861 cggcccatcg cggcacgatc ggtgtacacc ttttgtacca atgggtgcac tttgaaactc
     7921 tcaggaatgg tcgtgagtcg atctgctaat cgcttccact ctgtcaatgg tatggcggta
     7981 tcagcttgat cggtccactt cttatttaaa aatggtgacc aatcgactgc aaatttacct
     8041 ttgaagttac ttaataccgg gtcaagggtt tgcttaccct cttccatcgc agcacgaaag
     8101 gctttgacca tctcatcgcc tgaacctgca cctaacacac cttgtgcctc caaacgatca
     8161 gcgtataact ttcgtgtacc gggatgctta gcaataatgc tgtacatgta cggttgggtc
     8221 attgcagggg tatcttgctc gttgtgacca agcttacgaa agcaaatgat gtcaaccgct
     8281 acatccttac gaaattgcgt tctgtattcc aaagcgagtt gcgttgctag gactactgcc
     8341 tcaggatcat cgccattgac atgcagtacc ggcgcatcga tcgttttcat aatatcggtg
     8401 caatacaaac tcgaccgaag atctcgagga tctgaggtgg taaaaccaat ctgattatta
     8461 atgacaatat gaacggtgcc gcccgtggag tacccacgaa cctctgacag cgccaaggtc
     8521 tcttgcatca caccttgacc cgcgatcgcc gcatcaccat gcaccaagac gggcaatact
     8581 tgactgcccg aaagatcatt gcgccgctcc atgcgcgcac gagcggaacc ctcgactacg
     8641 ggattcacaa tttcgaggtg cgatggatta aaggccagcg
//
LOCUS       DDPQ01000066            4584 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_18471,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000066 DDPQ01000000
VERSION     DDPQ01000066.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_18471"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    174..183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1032..1126
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1214 a         1051 c         1016 g         1198 t
ORIGIN      
        1 ttgcacgttc cttataaggg tgcgggaccc gcattggtcg atttgatcgg taatcaagtg
       61 caagtgctct ttgataattt gccatcgtca gcgggtcata ttaaagctgg ccgcctcaag
      121 gcattggccg tgacctcaga aaagcgtgag ccctcattcc caaatgttcc tacnnnnnnn
      181 nnnggcactc caccagacat cgttaataag atgaacgcag caattaataa agcaatcagc
      241 caacctaaaa ccaaagagcg tcttgctgaa ctgggtggag cgctgatcgt tgggacccca
      301 gctgattttg gcaaagttat ccaagctgag accaacaagt gggagaaggt tgttaagttc
      361 tctggagcaa ctgccgagta acaaaaagct accccgcaat ataaacttga tacagtaggg
      421 ccgatgcacg tatcggccct tactgctttt tatctcacta ccgccgcatt gatgtgggcg
      481 ggcaatgcca ttgttggcaa gatattagtg caatctagtt cacccgtttt actcaatacc
      541 atccgttggg gagtaaccgc actcattctg ttgccatttg cttggagagt ctttggatct
      601 accagtccat tatggcaatc cagcaaacgg tttgcactac tgagtctttt gggggtaggc
      661 agttataacg tcttactcta cttggcatta aagagttcta caccaattaa tgtcacctta
      721 attggtgcaa gcatgccgat ctgggccatt gtgattggtg ccttgtttta taaagaacag
      781 ccaaacataa aacaaatcat tggtgcggtc atctccttaa ttggtgtaac cgtagtgatt
      841 gtccgtggag agcttgagcg tttgattgag attgagtttg tggccggtga cctgctgatg
      901 gttcttgcca ccatcttatg gggcgcttat agctggatgc taagtcatcc aaaagagagt
      961 accgagcgaa cctggccgtg gagctatttc ttattagcac aggtaggctt tggcttctgt
     1021 tggtctttgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcga cattctttac
     1141 aaacttaatt ccgctcttta cagccatcct ttcaaccttg ctattacaaa aaccccctga
     1201 gctatttcag ggagtcgcct ttgccctcat cttggctggt atttatctat cgattcagaa
     1261 gaagaaatca gtgggctagg acaattagag cggccgaact tgcacgcttc tccatttaat
     1321 cggaccgcct actgcacctt tgaccccagg accaaattgc agggcaatag ggccttcgct
     1381 aaagcgcgca tcgttggcgc gcgttgtttc aactccgttt aatttgacga taatctccgg
     1441 gccgttcgct ctaatctcca tgacattcca tttgccacct gctaaattgg taattggagt
     1501 tttcactgga gatacatcga caatcgcccc tgtaccaaat tttggttcag gacgaatatc
     1561 ccaaatattg acctcatatg aacttgcggc ggcgataact gtaggattgg tgacgcgaat
     1621 gaagataccg ctattggtgt cggtctctgc ccaaaactct gcccgaatct ccatattttt
     1681 atacgattgt ttggtgacca gaaagccaga tttgccctta tcggccacaa ttaaattgtt
     1741 ctccactcgc cagtttgcat ctccaacctg ttggaagttc tctaatcctt tgccgtcccg
     1801 aattaaatca attgaagagg tggagaggtg agagcaagcg gctagaagaa aagaactgca
     1861 gataagacca agtgttattt gagcaacgcg tctcatattt acccccgacg tttttgatgg
     1921 ttagattatc ttaggcgatc ttattcttta tgtctagaca tacgctaacc agcgtcccaa
     1981 accaatgacg ctggcccagc ccactagaga gatgactgct tgtactttag aggccgcatt
     2041 aattactacg cgcattcgca acaacacagc attggtaatt aacaagaaga tcagacacat
     2101 cttgagaatg aataaacgtg aggcaatcag atctccagca tgggtgataa atagcatcga
     2161 acctgtgagt acagccaaag agaacccgag tagagatact gcgattccta aacgcgagag
     2221 agcagagtaa tcaagagctt tgcctagacc caatacgcgt aaatccatta acaccacact
     2281 gccaaagagc ataccaacac cccaaatgtg cacagtttca acagtggggt agagccaggt
     2341 cgagtatttc atggcttggg ctagccaaaa gccctctaaa gccacgagtg gagtgctcat
     2401 taacgcaatt caatcgtctt attagaaacc gtaatgcgct cggctcgcat ctcatctggc
     2461 ttactacgat tcacatagcc ctctacagtg acctgggcac catccgcaat atccgttttg
     2521 agtaaacccc gattttccat ccgaaagggt ggggctaata ccgcaatcca ttctttgcca
     2581 tccgcactta acttaatgaa tccatgcggg tgttcatagc cactctgcat aaccttgcct
     2641 gtgagcttga ttaatttggt ctgctcgtac tccgaccagc catggtgagc gatcgagggt
     2701 aaggcaatta acagtgaaag actcatcaaa agaccgcgaa gcaataaagc cattttcatt
     2761 ttttatccct tgaatttaat cgccatatat tgatcttagc aataaagttt atagcgactc
     2821 tcatatctac aatgaggtct gtgtcttata ctgaaccttg attagaaagt gtaattggta
     2881 ttgaaagggt ttgggtcaaa tcacaacaca ttttttgaat ggtagaaaaa ttgcatagtt
     2941 ttttaaaatg agcgctaaga ttgaatttaa tcaaacttca acattgcgtt tgatgtcggt
     3001 tttatgtctg tgtatttctg atttatgtct gattaaagat cggtggcgat actttttcct
     3061 ttttttcctt ttgatggtct ccattgagtt gtttcgtgca aaactctata aggctctatt
     3121 gcatcatcat gaaacatctg ttcgtattga aaaatcagaa gccctaaaag agcataccgc
     3181 taatctagcg tcgattcaaa agagctctaa atagctgatt tagcccaata cccttgaatg
     3241 taatgaaatg agggttatta atccagcagg gatagtaaag cccaaaaagc gttctgtaaa
     3301 aaaacgaaat ggcaaattta gaggatgaat taagcacttc ctgggttttt catgtctgtt
     3361 gggtgccaaa aacaaacttc agaccagtta agcagggacg caagaaagag gacgagccaa
     3421 accctcggca ctgatagtta ttgggtatgg ctgcttcgtt cccgacctga ccaggttgcc
     3481 aagccaccat gcgaggaggc ccgtccatct ttattgtagc tcttgctaga attgcaatat
     3541 gactgcactt gcactagctc gtaaatggcg ccctaaaacc ttcactgaac tgcttgggca
     3601 agatcatgtg gttaaagcgc tgacccatgc cctcgatcag gggcgcttgc accacgcatg
     3661 gttatttact ggcacccgtg gggttggtaa gaccacgatc gcccgcatca tggccaaagc
     3721 gcttaactgc accggggcgg atggcaaagg acagatgacc tccaagccct gtggcaaatg
     3781 ccaagcttgc actgagatcg atgcagggcg ctttgttgac tatatagaga tggatgccgc
     3841 tagtaatcgc ggtgtggatg agatggtggc cttattggag aaggcagcgt atgcaccgag
     3901 tagcgcacgc tacaaggtct atatgatcga cgaggtgcat atgctgacct cgcatgcatt
     3961 taactccatg ctcaaaacct tggaagaacc accagagcac gtgaagttca ttctggcgac
     4021 cactgatcca caaaagatac cggtaaccgt tttatcccgc tgtctgcagt tcaatctcaa
     4081 acagatgccc gtgccagtga tcgtagagca tctgcaggct gttcttcaat ccgagtcggt
     4141 cagtgctgaa gtaaatgcct tacgggtcat cgcaaaggcg gcgcaaggat cgatgcgcga
     4201 tgcactatca ctcaccgatc aagccattgc gtatgcggca ggccctgtca ccgaggcagc
     4261 agtacgcacg atgctaggca cgattgatga tacgtatctc atcaagatat tggatgcctt
     4321 acaagccaaa gatggtaaga ccctaaccga tattgcagaa gagatgggct taggtagcat
     4381 gtccttctcc cttgcattac aagatctggc tagtttgtta cacaaaatag ctaccgcaca
     4441 gttagtgcct aactccgtac tcgatgattg gccagaagcc aatgagatcc gtcgtttagc
     4501 taaggtattt actcccgaag aaacacaact cttttatcaa attgcaataa ccagtcgtgc
     4561 cgatctgaca ttggccccag atga
//
LOCUS       DDPQ01000067           12618 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_12904,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000067 DDPQ01000000
VERSION     DDPQ01000067.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 12618)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12618)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12618
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_12904"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    5812..5825
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11574..11620
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3278 a         3171 c         2903 g         3197 t
ORIGIN      
        1 ccaggaagca aaacaatggc gattgtgccc aaaataaagc tgggcaaatg cacaattccc
       61 agctgctccc agttgatcca ataatccatg actagatcat aaacaggaac catgtcataa
      121 tggagagctt tttgaaacat cgcccccagc aatttacatt tttcagcgat tggtttttta
      181 ccccgctgcc gcttgtctga tggtcgatgc cctagaccat cgcgcctttt taccctggcg
      241 cacaaccgga gacatccccg atggggatgt gtaattgcat gtcaaattct gctgtagtac
      301 ctaactcatc ttttgagtct tttaacctcg caagtccgat ccttaaaaac atcgaagccc
      361 taggttatac ccaagcgacc cctgtccaag ctcaggttat tcctgcgacc ttggccggtg
      421 gtgactggct cgttagcagc caaaccggaa gcggcaaaac cgctgccttt cttcttccat
      481 tactcaataa actgtttatt gccaacccaa atggcagtcc cgttccagga cgtgcacagc
      541 ccaaaatttt agtgctctgc ccaacccgcg aactagccca acaggtatca gccgatgcta
      601 ttaatctggc tcgtggcatc aaaggtctgc gcattgcgac cattatgggc ggtatgccat
      661 atggcaaaca aatccaggca atcaaaggag ctagcttagt ggttgccaca ccaggccgct
      721 tattggatct cttcaatact cgagccattc gtttagacca agtggaatgc ctcgttgttg
      781 acgaagccga tcgcatgcta gaccttggct ttgctgaaga tcttgaggca attgatgagc
      841 gttgcaaaca acgtacacaa accttgatgt tctcagctac ctttgcccca cgcattatga
      901 gtttggcttc taatctcacc aaagatgcgg gtcgtattga gcttgcacat gctggtgaag
      961 cgcatgccaa tatctcccag aaactgcatt gggctgacaa tatggcgcat aagcatcgct
     1021 tgttagagca tctcttgtcg gatcccgatc ttgatcaagc ggttgtgttt gctagcaccc
     1081 aaatcgagag tgaaaagatt gctgatagct tgcgtgccaa tggctatcaa gccagcgcct
     1141 tgcatggtgc aatgcctcaa gcagttcgca atcgtcgtct tgattcttta cgtaaaggac
     1201 agactcgaat cttggtcgct accgatgtgg cagcacgcgg tattgatgta ccccgcatta
     1261 gtcacgtcat taattttggc ttacccatga aacccgaaga ctatacccac cgcattggtc
     1321 gtaccggccg cgctggacga aatggtattg ccattacgct tgcagagcat cgcgatagag
     1381 ttaagatcaa ggcgattgaa cgctttacgc aacagcctat ggccgctgct gagatcgctg
     1441 gtttagagcc tcaaaataaa ccgagcttta ataagccaaa ccatcgccct ggtgccggtg
     1501 caaaacgcgg caagcctaaa tttgcaggtc aaggtcgtcc gggtggttcc aatgcgcgct
     1561 ttagcccccg ttcgggttca cgttcgggtt cgtttcagaa cgctaatagc aaacactaac
     1621 tcttagcctt ctatgttgca gcggaagtct cgctctccat cgtgctggca ggtcattgaa
     1681 aatggtgcac ccttgggtgc accattgttc ttacaagact ggctgagtga cacgggctct
     1741 cttacccgca aattagaatc cacacttcgt tacaagcaag acgaacattt ggaactccgc
     1801 attctggcag actgtagaca aaatctagtc tcctccgaga agtcattctt taggcgcccc
     1861 ctatctcgct ctcgtattcg ggaggttgtt ttatgtgatc aaggtgagcc catcattgtg
     1921 gccagaagcg tattaccgat tagcagctcg attggtacca accatcgcat tcttaagcta
     1981 ggacataaac ctctgggtgc agttttattt gcaaaggatt tacgcggtcg ttttcgtcca
     2041 gtacgcgaga ttgttcgctt agatcatcat catcctgaat ggaaagcatg ccagagacgc
     2101 tttaaagatt tacccaaacg ggtatgggct cgccgcaccc tttatcagct caatggccac
     2161 ccgcttttgg taatggagat ttttttacct gctctccaag ccaaactaaa gcagcttggg
     2221 caatagactc atcacctgga tcacacactg ctacctcagt aaagccaatc tccttagcaa
     2281 cctttgcaat attaggatga gagcaaagag catggctctc tgcaaaagat ggaagaatgg
     2341 ttccaagatg acgcactgcc tccgatgaag ttaacaacca gagacttgta tttgcatcga
     2401 gtccctgaac tgcttgccac tgattactcg aagaatctaa agggatacgg gtatagatcg
     2461 ataaggcctc tactgcggca cctgcggctt gcaaatgatc caataaggac tcacgcccac
     2521 cctcaccttt cagtatcact actgaatacg cctgccaatc ctgaatatgg ttttggaggg
     2581 ctctccataa gccctctgaa tccgattgac tggcattggc cggcatcaca atcgcaccag
     2641 cctcaacccc atgtcgcaat agcgcatcac ggctactgct tccaacgacc ccgacccgtg
     2701 tttgcgcatt gaccagatcg ccccagacca ttcccgcatc tgtaatggag cgcatggtgc
     2761 actctatggc attagggctt acaaagatga taagtttggc gtttctaagt gcctttgata
     2821 ttgcagtaac agtccctgaa tcatttttag gaacaatggt gagcaggggc aggccaagca
     2881 catttactgc aattgcttga gtctgagcac cgccccggat taactcgatt aatcgtctcg
     2941 cttgaccact gggccgagta acaataatgg ctggatcgct agccatccca aatcctattg
     3001 cggtaataag gcggcagcac ctttagccaa tagatcatcg gcaatcgcga gtccaaaagc
     3061 ctcggcctcg tcataggagg caatcgttcg ctcgccagtt gctaaacaga tctgactccc
     3121 atccgtacta gcaacatagg aacgtaagct catctcgttg gcagatttcc actgggcatg
     3181 ggcagccaat ggcacctcac acgagccgcc aagtttgcgg gaaaccgcac gttcagctgt
     3241 tacagctaaa gcggttggta aatcgtttaa gggcgctaac cacgctttca cctcagggtg
     3301 agcgcttaag gtctcaatgc ctaaggcgcc ctgccctgcg gcgggggtgt ggggatcaaa
     3361 aggtaataat gctttgatac gagcactcat acctaagcgc tttaagccag cggctgccaa
     3421 aataatggct tggtactcgc cacgatcgag ttttgccatg cgcgtatcta aattgccgcg
     3481 caatggctct accttgagtt gggggtaatt gctccgaata atcgcttcgc gccgcaaact
     3541 ggatgttcca accaccgacc ctggtggtag ctccgctaga ctagcgtagt cattggatac
     3601 caaggcatcc cgagcatcct cacgtaccat tacacaggac aaatcaaagc cgggcggcat
     3661 caccatggga acgtctttga gggaatggac tgctaggtca gctcggccat cctctagggc
     3721 agtctctagc tctttcacaa agagaccctt tcccccgact ttggaaaggg ctcgatctag
     3781 gatttgatcc cccttggtgg tcattcctaa gatacggaca tcgcagtctg gatatagctt
     3841 gcgcaggcaa tcttggacgt gttcagcttg ccacattgca agccggctct ctcgggaggc
     3901 aattattaaa cgctttgggc tcataaggac gacttaagaa ttaaaatgaa tcattactgc
     3961 acacaatata ctgtgtttat gagctcacct aaaaattctt tagccaataa gtccaaagcg
     4021 tggtctggcc gctttgcaga accggtcgcc gccctcgtgc aagcctatac ctcatccctg
     4081 cacttcgatc aacgtctagc cttagtagat atcgcaggct cactagccca tgccgagatg
     4141 ctggccgctc aaaaaattat ctctaaagct gactttctag ctattcaaaa agggatggca
     4201 caaattgaga aggagattct tgcaggtaag tttgattggc aattagccct ggaggatgtc
     4261 catctcaata ttgaggctcg ccttaccaaa ctcattggtg atgctggcaa gcgtttacat
     4321 actggtcgct cacgcaatga tcaagtggca accgatattc gattatggct tcgtactgag
     4381 atagatcaga ttctggtaga tctcaagcgc ttacgtactg ctttgctcga tcaagcagaa
     4441 aagcatgcgg ccaccatcat gccgggccat acccatttac aagtggcgca accggttacg
     4501 tttggtcatc atctaatggc ctattacgaa atgtttaccc gcgatgcgca acgtctgaca
     4561 gattgccgtc ggcgcgtcaa tcaactgcct ttgggtgcgg cggccctagc aggcactagc
     4621 tatccgattg atcgggagcg ggttgccaaa acgcttggtt ttgaaggtgt ctgccagaac
     4681 tcccttgatg cagtctctga ccgtgatttt gcaatcgagt tttgtgcagc ggctgcaatg
     4741 ttcatgatgc acgtatcacg cctatctgaa gaactcattt tgtggcttag tccccgcttt
     4801 ggctttattg atctgccaga tcgtttttgt acgggtagct ccatcatgcc gcaaaagaag
     4861 aatcctgatg ttcccgaact ggctcgcggt aaaacaggtc gggtcaatgg tgatctgata
     4921 gcgttactca ccttgatgaa gggtcagcct ttagcgtata acaaagacaa tcaagaagat
     4981 aaagagcctt tatttgatgc agtggacacg atagcgcaga ccttgcaaat ctttgccgat
     5041 atggtgcctc aaatctctgt aaaccaagaa ataatgctta gcgccgccga ggagggtttt
     5101 gcgacagcaa cggatctcgc agattacttg gtgaaaaaag gtatgccatt tcgggatgcc
     5161 catgaatctg tagcgcatgc ggtcaagtta tgcgtcaaaa agaattgtgc tttgagcgat
     5221 ctaagcctag cggatctcat taaagcttgc ggcctaaatc ctaaatcaaa ctcaattgat
     5281 aaagatgtat tcaccttact aagccccaaa ggatcagtac actcccgtaa tcacatcggc
     5341 gggactgctc caaagcaggt gctagctgcg attaagcgcg ctcgcacttc gctaaaaaaa
     5401 taattggggt attacaacct aggcgcgaac gcagtctacg tagtagccgt tatcgccttc
     5461 agcgttcttc acgagcccat gaatatcggt ctcaaatcca gggaactgtt gattaaagtc
     5521 acgggcaaat cgcaagtaat caacgattga tttattaaaa cgctccccag ggattaggag
     5581 tggaatgccg ggcggataag gagttaccag cattgcggta actcggcctt ctaactcatc
     5641 aatggggact cgatcaatgt ctttatgagc catcttgctc catgcatcag ccggaatcat
     5701 ggcgggctcc atattggata aatacatctc cgtggtcatg cgcgctacat cacggctctt
     5761 ataaaaagaa tgaatctgtg cggagatctc ttgcaggcct actcgttcgt annnnnnnnn
     5821 nnnnnttaaa ttgctggagt tcggtcacca aggtgttcca gcgccctttt gtaatgccga
     5881 tggtgaacat aatgaagaac gaatataagc cggtcttctc cacgatcacg ccgtgctcag
     5941 caagatattt ggtaacaatg ctcgccggta tcccaatctc tgcaaaatta ccttcgatat
     6001 ccaggccagg agtaacgatc gaagctttaa tcggatcgag catattaaag ccctgggcca
     6061 gattaccaaa atcatgccaa taggccttgg gttctaagat ccaatcttca cgcgtaccaa
     6121 taccctcatc cgctaaatga tcggggcccc agaccttaaa ccaccagtca gcgccaaact
     6181 catcatccac cttgcgcatt gctctacgaa aatccaatgc ttccgcaatc gactcttcca
     6241 ccaaggtggt gccgcctggt gaatccatca tggcagccga gacatcacag gaagcaataa
     6301 tggcgtactg tgggcttgtt gaggtgtgca tcaaataagc ctcattaaag caatcgcggt
     6361 caagcttacg ttcttcagca tcctgcacta aaacctgcga ggcttgtgag aggcccgcta
     6421 ataatttatg ggttgattgg gtcgagaaca tcatgctctt cttagtgcgc ttacggtttt
     6481 gcccgaaagc gtgcatatct tgatagaagg gatgaaaggc ggcgtgtggt aaccacgctt
     6541 catcaaaatg caagctgtct accttaccat cgagcatcga cttgatcatc tcgacgttat
     6601 acaaaatgcc atcataggta ctctgtgtaa tggttaatac acggggtacg acattcttat
     6661 ctttaataaa ggggttggca tcaatcttct tcttgatgtt ggcccactca aactcctctt
     6721 tgggaatggg gccaataatg cccaaatgat tccgggttgg cattagaaac actggtatag
     6781 cgcccatcat catgattgaa tgaatcactg atttatggca attgcggtct accaagacca
     6841 cgtcgcctgg ggcaacggtc gaatgccaga caattttgtt agaggttgat gtgccattgg
     6901 tgacaaagaa caaatggtct gcactaaaga ttcgggcagc attgcgctca ctcgctacta
     6961 ctgggcccgt atggtctagt aactggccaa gctcttccac cgcattacaa acatcggcgc
     7021 gtaacatgtt ctccccaaag aactgatgga acatccggcc aacaggactc tttagaaaag
     7081 caactccacc cgagtgtccc gggcaatgcc aagaataaga gccctctgag gcatagtgtg
     7141 tgagcgctcg aaagaatgga ggggctaaag aatctaaata caccttcgcc tcacgaataa
     7201 tatggcgcgc tacgaactca ggagtatctt cgttcatatg aatgaagcca tgtaactcgc
     7261 gcaagatatc gttaggaatg tggcgtgagg tccgggtctc accgtacaag aagatcggaa
     7321 tatcttcatt acgctttcgt acctcgacca caaaggagcg tagattgctg atcgcaggaa
     7381 gatcactttc gtctgcatca attgctgcat caaactcttc atcatcaatc gacaaaataa
     7441 agcatgatgc acgtgaggcc tgttgtgcaa acgaggtgag atcaccataa ctggtgaggc
     7501 caatcacctc aatcccctcc ccctcaatgg caagtgctaa atcgcgaatg cctgaacctg
     7561 aaatattttc agaacggaag tcttcatcaa tgatgatgat cggaaaacga aatttcatga
     7621 tgactaggtc tttggtagag taacgccgtg ctgaccttgg tatttgccgc cgcggtcttt
     7681 ataggaggtg ccacacactt catcgctctc gaagaaaagt acttgagcgc acccttcgcc
     7741 tgcataaatt ttggcaggca atggggtcgt gtttgagaac tcgagggtga catacccctc
     7801 ccactctggc tcaaatggtg tgacgttcac gataatgccg caacgtgcat aggtgctttt
     7861 acccacacag atagttaaga cacttcgcgg gatcttgaaa tactccactg tccgcgccaa
     7921 tgcaaaggag ttgggcggga tgatacagac atcacccttg aaatccacaa acgatttttc
     7981 atcaaactgc ttaggatcaa caatggtgct gttgatgttc gtaaagatct tgaactcgtc
     8041 ggcacagcga atgtcatagc catagctgga ggtgccataa ctcacaattt tttggccggc
     8101 gtcattggtg cggatctggc caggctcgaa ggggctaatc atcccttcct ctgccatccg
     8161 ccgtatccag tgatcagatt taatagtcat ggcgggattg taagactttc atgcccaact
     8221 gggcagaagc gggggcttta aaggcctagc ttgtctgaaa aaccacacgc tctggggcgt
     8281 tatagatctc aaagtgctta cccgaacagc gggacagtaa ggtcaggtgg gtcttgcgag
     8341 ccagttctaa gcccatttgc gtgaccccag aacgggtgag taggaaggga atacccatct
     8401 gagccccctt gatcaccatc tcggaggtca ggcggccagt ggtaaagaaa atcaggtcat
     8461 cgccttgacg gtttgccaac cacatcaggc ccgagatcga atccacagca ttatgacggc
     8521 caacatcctc gattagatga aggagctgca ccttttctcc ggcgcgctca aacaccgcac
     8581 aggcgtgtac tgagcctgct tttttgtaga tggtttcatg tgcccgaatc tgctctagca
     8641 gatctacaat cgcagcttga tgaagtttag gaccctctgg taagcgaatt tgtgccatat
     8701 cttccatcag gcccccgaac atggtgccct gaccacagcc agtggtcacc acccgtttgc
     8761 tggtgattgc ctcgatatcg acagtgcttc gccgtgtttt aactgccgca gaatcggttt
     8821 cccaatccac ctggatactt tcgatatctt cgggcgactc caccaaacgt tgattgcgta
     8881 aatagcccaa taccagggcc tcaggagcag atcccagggt cattaaggtg acaacctctc
     8941 gtttgtcgag ataaatggtc agagagcgct caccggggat aaacgtaggc ttgcgacgcc
     9001 ccaactcgtc gataatctcc acctcatgta ccaccggtac ggaggcatta ctcattgtga
     9061 ttgggtgttc cccgcttgca gccattgtgt ctccatgatg ccaaactaaa ccctattttg
     9121 agcttatttt gatcatttaa ccattgttgc cgaccatcac tttaaccgca gactaaaatc
     9181 attgctgaat cgataaaaat aaatatgcgt caacttctag tcacttccgc cctgccctat
     9241 gccaacggcc aaatccacat tggtcatttg gtggaatacg tacaaaccga tatttgggtg
     9301 cgctttcaga gaatgcgtgg tcatgaggtc cattatgtgg gcgccgatga tacgcatggt
     9361 acgccgatca tgttgcgcgc tgagaaagaa gggatcagcc ccaaagagct cattgataag
     9421 gtctggcatg agcacaagcg agacttcgat cactttttaa tttctttcga tcactactac
     9481 accaccgata gccctgaaaa tgagatgctc tcacgcgaga tctacctcaa attacgcgat
     9541 gccggtttaa ttgagaagcg cgcgattgag caagcctacg atcccgttaa agaaatgttt
     9601 ctgccagatc gtttcattaa aggtaactgt ccaaaatgcg gtgccgccga tcaatatggc
     9661 gattcttgtg aaaaatgcgg cgccacttat gcaccaaccg atctcaaaaa tccgtattcg
     9721 gtggtgagcg gcgccacccc cattcgaaaa gtatcggatc attatttttt caagctgtca
     9781 gacccccgtt gcgaatcctt tcttagggag tggactcaag taaagacccc cttacaaagc
     9841 gaagcaaaaa ataagatgaa agaatgggtt ggcattgatg gagatagcaa gctgggtgac
     9901 tgggacatct cacgcgatgc gccttacttc ggctttgaga tccccgatgc accgggtaaa
     9961 tatttttatg tctggctcga tgccccgatt ggctactacg ccagctttct gaagtactgc
    10021 aaggctaagg gtctgagctt tgatgattgg gttaagcctg acagcaagac tgagcaatac
    10081 catttcattg gtaaagatat tttgtatttc cacactctat tctggccggc aaccctgcat
    10141 ttttctggtt accgtacccc caccaatatc tttgctcatg gcttcttaac agtggatggt
    10201 gagaagatga gcaaatcacg gggcaccttg atatctgcac atagtgtgat tgaagcaggc
    10261 cttaatcctg aatggttccg ctattacttt gcgaccaagt taaatgccag catggaagat
    10321 cttgatctca atttgcagga ttttgtggcg cgtgttaata gtgatctctt aggtaagtac
    10381 atcaatatcg cgagtcgtag cgctggattt ttagtaaaac gctttgaggg tcgagtctcc
    10441 gatagcgcca tggctcatcc ccttcttagc accatcacca atgcaaatac aagtattgct
    10501 gaactgtatg agtcacggga atatgccaaa gcaattcgcc aggtcatgga actggctgat
    10561 ctagtgaatg gctttgtgga tgagaacaaa ccatgggatc tagctaaaga tcctgcccgc
    10621 agtgatcaac tgcatgaggt ttgtagcgta atcctcgagt ccttccgctg cttgagcctt
    10681 tacctcaagc ccgtattgcc ccagctcgtt gccaatgtag agattttctt gggagtgcca
    10741 acaatgcaat ggcaagatat ccaaaccccc ttgcagagca gttcgccgat tcaaccctac
    10801 aaacatttga tgacgcgggt tgagcaaacc cagcttgacg aactactaaa actcaatctt
    10861 taaggcccca tccaaaaatc gttaataatg gggggtagta gattctgttt tattccataa
    10921 cctattgatt ttatgaagaa atataaatct caagtcactc aatttttgga gcaactcaag
    10981 gcccaaaaac cgcatctcga acaatcgcag cgagaaggtc gagccctctt atgggataaa
    11041 actcctcaaa accccgaaga gatgcgacgc accaaactag cccgcattaa acagcgttct
    11101 tacgtttaca gtaatgagtg aggagctcga atctctttct aatggaccat ccgaagaaaa
    11161 aattgatggt ttaagttctg cgttcgccaa actctatggc gagcccttat ttaaattacc
    11221 gaccgattta tacatcccac cggatgccct agaaatcttt ctggaggcgt ttgaaggtcc
    11281 gcttgatcta ttgctgtact taattcgtaa gcaaaacttc aatgtgctcg atattccgat
    11341 ggctgaggta acacagcaat acttgagtta catcgatcaa attcggcatc acaacttaga
    11401 gctcgcagcc gaatatttat taatggctgc catgctgatt gaaatcaaat cacgcatgct
    11461 attgcctatg aaaaaagcag atagcgatga agaggttgat gacccgcgcg ctgagttggt
    11521 tcgtcgcttg cttgaatacg agcgcatgaa attagccgct caagaactag atcnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acgtggcctg aagttaattt
    11641 agaagaccta caaatggcat ggcgtgatgt actccatcgt gccaaactca atcaacatca
    11701 caccatcacc cgtgagcaat tatcggtccg agattttatg acccgtatct tgcgccgttt
    11761 acagaacacc cgctttgttg agttcagcga actctttgag gatgccatca aggctggcaa
    11821 cggcatccca gtgattgtgg tgaactttat tgccatgctt gaactctctc gcgaagcact
    11881 gattgagatc acccaagcgg aaccctacgc acccatctac gtccgcttgg cctatacccc
    11941 gagcgcatga aaatcgttac agacatccat gagctgcgtg atcacttgcg cggtcaaaac
    12001 cgggcttctt ttgtgccgac tatgggtaac ctccatgagg gtcacctttc cctcatccga
    12061 atagctcgcc agcatggcga tccggtaatc gcaagcattt ttgttaatcg tttgcagttt
    12121 ggnnnnnnnn cgggttttat accccgagcc acaagagtat cgtgttgacc cgcccaagca
    12181 attgggggat atcttggaag gtgagttccg acctggattc tttaaaggag tttgcacagt
    12241 tgtactaaaa cttttttctt gtgtccaacc caaagtggct gtctttggta aaaaagatta
    12301 ccaacaatta atgattattc ggcagatggc acaacaattt gctctacctg ttgatatcgt
    12361 tgctggagaa acgattcgtg cggaagacgg cttggccctc tcctcccgta acagtttttt
    12421 atccaccgaa gagcgtaagg aagcaccgca gttacagatt gcactacaaa atcttcgtca
    12481 cgagatccaa acgcttcagt cttcatcgct agctcttacc ccaaatcaat tgatggcatt
    12541 ggaacaaaaa gtgattgaga ccctaaaagg tcgaggttgg gctgctgact atattgctat
    12601 tcgtaaacgc actaatct
//
LOCUS       DDPQ01000068           12990 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_16925,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000068 DDPQ01000000
VERSION     DDPQ01000068.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 12990)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12990)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12990
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_16925"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1058..1119
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1548..1596
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3140..3176
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7358..7381
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9337..9387
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11218..11295
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3379 a         3127 c         2912 g         3271 t
ORIGIN      
        1 gtttgtggta acgacgtttt agcaattgga gcccttttag aagctcaaag ccaaggtata
       61 aaaattccat accaatgctc tattcttggc tttgataatt tagagctctc ccgccatata
      121 caaccaagtc tttcaaccat ccatattgac gcaatcggca tgtggtctaa agcagcccat
      181 catttaatgt cccaaattaa tggaatcaac agacttccta gaaaaatatt ggctgatgta
      241 agtttagtta ttagagactc taccgggggt gtactcaaaa cttaattacg aatccaagtc
      301 ttgatttgat caatggtttg ctgctcaatg tcatgaaagc catgataaga aaatgcttgg
      361 cacgcatcac ccgttgagct cccaccttcc atggtaatca aaggtgtttt ataccgctca
      421 tgattagatt gggtactggc ataggtggtc aaatggcatg gatcattttt atgatgaacc
      481 attaggtgtc tactcttgaa cttgcgtgta tcaaagcggg caatttggtt catggatgct
      541 gtatgaacga tgcaatcgat cttaccgtca aacttttctg ccaatcccat tgtggagtag
      601 gtactccgac tggttcccac catacagatt ttggcatttg ggaagcgtgt ccctaaatca
      661 ttaattaagg cacccatcct ctctgggctc tccgttgaat tagttgaggc tgttgcaaat
      721 tgcgaatcac aaaaccggtt cctagaacga atcaggaagt ttcctgcgaa gtcaaattgc
      781 aacttaccct cattattgat gtgcggattg agagtaccag ctcctccggg cattaagatt
      841 aaaacgtact ggggtgattg attttgacta gttaagatgt atggaactgt aaattcattt
      901 gaatacttca cattatcaat aagctgatca gtacagcttg catgtagatg caaacttgta
      961 agagacaaaa tagcaaagac tattaatctc atgaggctac tgttcaatag gcaatctgtg
     1021 cgattaccgg cgcatgatcc gatggttgct cccagccnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gcctaaaccc catcatgcga
     1141 tagtcccacc agctataggt cttgggtgct tgttcaaaga gtctgaaaga gtcatgcagt
     1201 cccaattgaa tcaaattctg aaactgctct cgctcttggg gtgatactag attttgccct
     1261 tcccaggcct tggggtcatg cacatcctga tcttcggggg cgatattaaa gtctcccaag
     1321 agtgctagac gtggataggt tttcagctcg gtggcgagcc atgttctgag agcatgaagc
     1381 caattaagtt tataaacaaa cttatcacta gcgggatctt gaccatttgg aaaataagcc
     1441 gagattaagc gcatcggtgg catctcttta aaacaaacgg tagcagcaag gatgcgttgt
     1501 tgctcatcgg caaagttggg gatgttctta ttgacttgta agaatgcnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttat aggttttttg accattacta
     1621 atgctaatgt accctgccgc ctcaagctca cgatggggat acttatcatc tgtcagcttg
     1681 agctcttgca agcacagcgc atcaatcggg ttttgtgctt ttgcctgact ctctagccac
     1741 tgaataacgt gcggtagacg caccttgatg gaattaacat tccaggctgc tatgcggatt
     1801 gatttttgag cgggcatgac aagattgtaa ttgccatacc cggtccggat acttatacgt
     1861 cgatttggag ctcttgtaac ttacgggtaa tggtattgcg accaataccc aggcgggtag
     1921 ccgcttctac cttgcgacca cgggttactt ccaatgcagc catcaagacc gacttctcaa
     1981 agcgagccaa gagagaatca aatacttctt gatcgttgtc ttgtagcatt tttactgcca
     2041 agcggttaag gtttccttcc cactcagttc cagttcgcga tgggctaaca ccctcaacga
     2101 ttggaatggt cgcagcactt ggagcggcat tggaactggc aacgggcgta ttggctgata
     2161 gcaatagatc ttctgggaga tcattaactc caaccactgt agaaggtgac atcacggtca
     2221 tccaatggca taggttctca agctgacgca cattaccagg gaaactcatt tgaccaatga
     2281 ccttgagtgc ttcatcggat aaacgtttgg cctctacacc taaagacttt gcactcgaca
     2341 ccataaaatg acgtgctagc gcggggatat catcggcacg ctctcgcaac gcaggcaggc
     2401 gaatccgaat cacattcaag cgatggagca gatcttctct aaagagacct tgggcaacac
     2461 gagcctctag attttgatgc gtggctgcga tgatacgcac attcgatcga attggatcat
     2521 gaccgcccac tcggtagtaa tgcccatcgg atagcactct taagagacgt gtttgcagat
     2581 caaacggcat atcgccgatc tcgtctaaaa ataaggtacc gccttcggct tgctcaaagc
     2641 gaccacgacg cagtgcttgt gccccagtaa aggccccacg ttcatgccca aagagttctg
     2701 actccaatag atcttttgga atggctgcgg tattcaaagc gatgaatggg cccttcgcac
     2761 gtgggctgtg tttatgcaag gcatgtgcta ccaactcttt accagtgcca gactcaccag
     2821 tgatgagaac agttacttgg gattgtgaga gacgaccgat tgcccgaaat acctcttgca
     2881 tcgcaggtgc ctgcccaata atctcagggg cttcttgtaa ccaggcggcg gattctttgg
     2941 aaggagcctg agtgcgctta ccctctccaa ttgcacggtg gattaactca acagccttat
     3001 cgatatcaaa tggtttggtc aggtactcaa atgcaccgcc ctgaaacgat gacactgccg
     3061 actccagatc agaataggcg gtcatgatga tgaccggcaa gttgggatgg ctggccttca
     3121 cgtgttgtag gagatcaagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggg
     3181 cattgagcac atcattggga ttgctaaagc tgcggtgcgg gatcttctca cgactcagcg
     3241 ccttctcaag cacccaccga attgactgat catcgtcaac aatccaaatt ggtttcatca
     3301 ttgcgccatc tcctgcttcc gataaggaat ctgtatatga aaatccgtat aaccaggacg
     3361 actctcgcaa gcgataaatc cctggtgttg ctgcacaaag gtctgtgcca gcgttagtcc
     3421 caagccactg ccgccctccc tgcctgacac caatggaaaa aatatgcgct cacgaatctc
     3481 atccggaata cccggtccgt tatcaattac atgcaaatct aatgccaact tgtgtcgttg
     3541 ctttgcaatc gtgaccgaac gagccactcg cgtgcgcagt tcaatctggg ctgtgcctct
     3601 gcgaatctca tcaccaagag cttgtgcggc gttatgcaca atattcagta ccgcctgaat
     3661 taattgctct tgatccccca aaatatcagg caagctggtg tcatagttac gaatgatctt
     3721 caagccctga gggaactcag ccaagaccaa gcttcgaatc cgttccaaga cctcatgaat
     3781 atttaatgga tccatcgcgt gtgctttgcg atgaggggca agcaatcgat ctaccaaggt
     3841 ctgcaaacga tcagactctt taataatgac ctgggtgtac tcccgcagtc ccttctcagg
     3901 taactcaaac tccaggagtt gtgccgcccc ccgaatcccc cccagagggt tcttgatctc
     3961 atgggctaga ttacgcatga gttgcttatt agcctcaacc tgatgggaga cccgctcatc
     4021 acgttcactt ttgagttgct gatcaatcgg gaaccactcc atcatcatca gagtggggtc
     4081 ctcaagcatg gcaatcacta catggactgc aaggggctcg cgataaattt ttccggggac
     4141 agaatacaag acgagttctt gacgctgggc taccgcccga ccggcataca cctcatcaat
     4201 catatgattg agcgcttggt tctcgccaaa caggtcgtag agggtatgac cacgtaggct
     4261 cttccgagaa aggtccaaag ctgcttctgc tgcagcgttg gcatagacta aatctttggt
     4321 tactccaata aataccaata cggagtttgg catttgatcc aacatgcttg aaacaaaagg
     4381 ggcggccggt gtagtgacag cggcagcccc tttacttaat ggattacgca acatggagcg
     4441 cgcaacctga ttaagaaatt acagcgagta gtacatatca aactcgatag ggtgcgtggt
     4501 cattctgaac ttggttactt cttccatctt cagatcgata taggcatcca acatcgaatt
     4561 tgtaaatact ccaccgcggg ttaagaactc gcgatcctta tccaagcact ccaaggcttg
     4621 atccaagcta tggcatacgg ttgggatctt tgcatcctct tcaggaggca ggtcatagag
     4681 gttcttatct gcagcctcac ccggatgaat cttgttctgc acgccatcca agccagccat
     4741 taacaaggct gagaacgcca agtatggatt tgctaatgga tcagggaagc gggtctcaat
     4801 ccggcgtcct tttggatttg aaacgtacgg aatccgaatg gaggcagaac ggttacgggc
     4861 agagtaagcc agcttcacag gagcctcaaa gccaggaacc aaacgcttat aggagttagt
     4921 gccagggttg gtaatcgcgt ttagtgcgcg ggcgtgctta atgatgccgc cgatgtaata
     4981 gagtgcaaac tctgacaaac cagagtagcc attacctgca aacaagttct cgccattctt
     5041 ccaaaccgat tggtgaacgt gcataccaga gccgttatcg ccgacaacag gcttaggcat
     5101 aaacgttgca gttttgccat aggcatgggc tacgttttgc accacatact tctgccaaat
     5161 ggtccagtca gcgcgttgta cgagggtact aaacttcgta cccaattcgt tttggccttg
     5221 tccagccact tcatgatgat gcacttctac tggaatacct aatgactcca gaatcagaca
     5281 catctctgaa cgcatatctt ggaatgtatc gacaggagca actgggaagt aaccgccctt
     5341 cttgcctgga cgatgaccgg tattaccacc ttcaatatca gcgcctgatg accatggagc
     5401 ctcttccgaa tggatcttca ccgagcagcc ttgcatatcc acattccact gcacgccatc
     5461 aaaaacaaag aactctggct ctgggccaaa gtaagcagca tcacctaaac ccgagctctt
     5521 caaatacgct tcagcgcgct tagcgattga acggggatcg cgatcgtagc ctttaccatc
     5581 agatggctca atcacatcac acgtgataac caaggtgggc tcttcataaa atggatccac
     5641 atacgctgca gttgggtctg gcatgagcaa catgtcagaa gcttcgatac ccttccaacc
     5701 agcaatcgat gagccatcaa aagcgtgacc gctctcaaat ttatcttcat taaatgcaga
     5761 gattggtact gagacgtgct gctctttacc tttggtatct acaaagcgga agtcaacgaa
     5821 ggtgcattcc ttctctttaa ctagtttcat tacgtctgcg acagtttttg tcatgcaact
     5881 ctcctctatg aatgaagtaa tttggaactc aagcgactgg ggattaatca agccaacaac
     5941 ggatttaaat catgaaagta atttactcgt gcacagaagc actatttagt tattttgcac
     6001 tattttagtg aaaaaagtgc ttatttagat atttatgcac ctatttagtg caaataagat
     6061 gatttattgg tgaatataaa cctcaaagcc taggtcagcc gtcccctgtt taagcttctc
     6121 aagggcctgg ggaaccaaat gcgcctcccc ttttacccct aactcaatat gacgtttagc
     6181 aaacacacca ccccgacttg gatcgccgac cgagggcaaa ctaaagacct tcaagccttg
     6241 gaactcgtgt tcaactgttt ccatcaaagg agttagggtg gactcaatac cactgggaac
     6301 aataaaactt tgctccagcc aattgctctg atggaataaa tctgcgtagt gatgatcgag
     6361 gcaccacgcc atcatcggcc ccgccatcac cggaaatccg ggtacaaagt gatgttcagc
     6421 aatcctaaat ccaggaattt ggttatacga gttcggaata atctcgctgc cgaccgggaa
     6481 ctctcccatc ttgaatcgat gttgattctc tggacgattt aaatccgctt tgatgggatc
     6541 accttcagca ctggtcacaa tccgcgccgt aatcagctct ttagcttgtg ggtgtagcgc
     6601 cagttgcaag cccaatgcat tcgctgctgc agctcgtgtg tgatcatccg gtgttgcacc
     6661 aatcccaccc gtactaaaga ccacatcgcc actcgcaaag ctttgcttaa gggtgtcgat
     6721 aatttgctca gggtcatccg ctacatagcg ggcccatgac aaacttaatc ctcgctcaga
     6781 gagtaactca atcatcttcg ctagatgttt gtcttgacga cgtccagaca aaatctcatc
     6841 gccgatcacg atcaatccaa agcgacgact ctcattctta gccatcgatt actccagaat
     6901 ttgtagagcc cgcttgattg gtaatgatat ttgcagtggt atcaatcgtt ttagcctctt
     6961 cacgagcatg ctgtgcatcc ctcaactgct ttaaggcatt taataaataa tgactaaacc
     7021 acaaggctgc aaagataaat attaaggaat aaatccacaa cgctataaag gagacgaagg
     7081 ggaagagaac taaagcaagc gcagaggtgg cccaaaagaa agtgggtaca gcaccaaaca
     7141 tccctgcaac cacacccata cctaatagag tccagcgatg agtctctaac agttgctcac
     7201 gctccaaaga actcgcatga tttgctaaga cgtcataaga catcaaacgc atggtcaacc
     7261 atccccataa gagtggggga aagatggcta ctaaaggtgg aatccaccaa actggcaacg
     7321 ttagcaaaac cagtgctaag caaatcagcg cagaccannn nnnnnnnnnn nnnnnnnnnn
     7381 nacagtggag aacgcaataa ataccaataa gctaatggcg atcaccggaa ttaataacat
     7441 caccaagaac aatggggcaa tccaagcgcg cgcttcgtca aagccgacca tctccaacgc
     7501 attctgaatc cagcttgtaa agatcgatgc ggtaaggaag gttcttaata actcaagagc
     7561 gggcgaccaa atgagccaaa tgacacaacc ccaaaatatc gacacaatca gaaaaggtct
     7621 aaagctaagc catagcatct tgggatgcat agtgccaacc aatgctaagc cgagtgaacg
     7681 aatgacctct cccatcatca caccgtctgt tttagccttg cctcaatgca cttcattgga
     7741 ttgatgaacc tgtgaacgcg aagtaaatag ttgctgaaga gaatggcgca agccctgcca
     7801 ttgctgcaca agtagattgt tcgagacagt taagtttcgt actcctgttg ggtaggcttt
     7861 atcgacaaac actgctgtac caaaagcgat atcccaccac ggaaaaagta ctccaaaatt
     7921 acagccccct aagacccctg gctttccggg tgcatcgtgc ccaaagccca ccgcatggtg
     7981 ataacgatgg aataaaggag aaacgatgag gtagcgagcc caccctaggt gaaccttgag
     8041 attagcgtgc tgccaacttt gtatgagttg gctgatcacg accaagaaaa taaattgtcc
     8101 ggggggtaca ccaaatagca aagcaaagaa ggcgaagacc attgcacgca tcacatcgtc
     8161 aagcaggtga ttacgatcat cagaccatgc ggtcatcgtt gtttggctgt gatgcaaagc
     8221 atgcaattgc caccaccaat gaatacgatg agaggcgcgg tgatagaggt aatcaatgaa
     8281 atcgaggagg atgagataaa tgaagaaact aacgatgggg atcgatgtaa atccaggaac
     8341 ccagttttca acaatcaatc gctcaaagcg gatatcgtgc aagagggatg agatctgaaa
     8401 aaagattccg gcaaagagca ggaagaatat gccatgaaat actcccagtc gatgaatcag
     8461 ggtatagagc atatcgatac gcacattcgg agcagagttt ttttgttgct ccgcaggcca
     8521 gaacttttcc caggttctaa agatcacagc aatgatcagc aattgaatgc atcccaatag
     8581 aaaccattcg gtaccatcga agacatcttc ggcccaggcc atccagccca ttgcaaataa
     8641 gatgggttcc acgacatggg cataaagcat gccatgcagg gaactatagg cttcttgaat
     8701 ttgttctaga aagcgcatcc ctctattatc gtcaatttgg tatcagagcg aagcaaaagc
     8761 cgcgtttttt ccagttctcg atcaggggct caagaaccgc tggggcccag gggtcctctc
     8821 tagaccagat ccccaaatgg gcgatggcaa tatcaccatt ctttagaccc ttactggcct
     8881 tgtcgagcaa agccgcattg ggataacgtt tggagtctag ttcgtccccc aaaaagccac
     8941 tcgctgacca gccgatatgc ttgtaaccac attgttcgcc catagcaatt aagcgctcgg
     9001 aggtttttcc ccctggagct cgccaaatag gatcaatagg tttgcctgtg agctcctgaa
     9061 aacgctgact cactcgttta atttctgtgc agagttcggc tggattaaaa cggattgctt
     9121 tacccgcatt gtttccaaac tgaggtctca gaatgacatc agactcacca acatctttta
     9181 cccagtaact atggtcatag gtgtggctac caaaacgatg accttctttg acacgctctt
     9241 gccaatagct tttccaagaa tcgtccatgg agtaaccgcc ctgcttggtc ttctcattgg
     9301 ccaaaaagaa ggtggccttg atcttgtttt gttgcannnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnngta atgagagatc ggagactcac tcgcatcatg
     9421 ctcatgggct actcccaact ggctcgagga tagaagaaca caccatgcgg tgagcgcccc
     9481 actggaatca catcaatcaa cttcatagag ggaacatcga tcacccctac ttttttagag
     9541 aagcgcagtg tgacccacaa tgttttgcca tcgggtgtaa gttccatatc atcaggacct
     9601 gctggtaggc cagtaatatc gcccaccttc tccagagttt gcatattgag caaactaata
     9661 gtggatgcaa cacgattcgt tacaaagaca tgcttcttat cacctaaggg tcggaagtta
     9721 tgtgctccat tggcggtctt aatccgctta atctctttcc gattacgcca atcaatcact
     9781 tgcacataat cagcaccggt aatccccacg agtagatact gatctttagg ggtcatccac
     9841 aaacccgcag gcaccggacc agtgggcata cgccatatga tcacctgttt ctctagatca
     9901 atcgcgacta cctcagcaga atcttgcaag gtcacaaagg caagcttgct atccgatgta
     9961 aatgcaatat ggcttggggt ctttgcggct ggaatggatt tggctagttt gaactcagcc
    10021 ccattggcgg catacacatc cacacggtct aagcgattag cagcagtcac aaaccattta
    10081 ttgtttggag aatatccaat ttggtaagga tcaataattt tggggatacg cccagtcatg
    10141 tcgccagtga tggggtttaa taaaacaata tcgtccccgg cggcatttgc aacgaggagg
    10201 gttttctcgt ccggggtcat catcaaatga tgcggctctt tacccacatg aaaggtcttg
    10261 gtgaccttgc ggcttgccat atcaatcagg ctgacagttg cctctccgga gttcagaata
    10321 atcgcaagct ttggctctgc agcaaatacc aataccggtt gaagtaagag taaagcgcta
    10381 ataagagaga tgagacgggg ttttagtaaa taggacatag cgtgattgta aggcccgctt
    10441 cgctttgatg agtattgctc ataagcctaa aggcaatagg tttattgttt tattggggta
    10501 ctaacgcaga ctggccaata ccttctcgag gcgcttcacc gacatggggg ttggggttct
    10561 taactcttga gcgaataagg ccaccctgag ctcttcaagt tgccaacgga aatctttgat
    10621 tcgggggtcc tgttcaagct gatagtgacc agaacctttt gcaccttggc gcattttttg
    10681 ccatggcctt gccaaggact cccaatcctt ttggcattgc gcatcccgcg ccgggttgct
    10741 gcgaagtttt tcaatacgca ggctaatggc tttgagataa cgaggtagat gcactaattg
    10801 ctcatagggc gtttgcccta aaaattgcgc atgcacaaga ccttgtaatt gctggcgaat
    10861 atcttcgtga gcgctactag atagcgcctt agcgctagct aattttttac taagctccgc
    10921 atgctcttcg agggttcgta gtacatgctt agcaatctct ccggcaatta agacaaagcg
    10981 aggtttaccc ttttgtaaag attcttcaaa ctctgctttt gtgcttgcta agccgtcttg
    11041 cataaaggct cgctcgatag ccatcatcac tatctcttca atcaaggcct caaccgatcc
    11101 taattgcata aagagtaggc caatatcgcg agcaccgggc agttgtttat ggagcgcttt
    11161 gacgttctct ctgaggacca aggcatagag tcgttttaag cctaggcgat ggatttgnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnctcgg acttactttg cctacctcgc tttgcttagt ttgaccttga
    11341 ctctcctggt cgcttccatc atcgcttgcg ttcatacttt tttgtgcgag gctctgaaaa
    11401 acctctctag caatggatcc aaactccgtg cgtaacttca ctaaattccg ctctaggtcc
    11461 atttgtctgc cgtgctcatc aagcaagcga aagttcataa ggcaatgggc tggcagggtc
    11521 tctggtctaa agtcggcccg cttgatatcg attgctttac tctcccgcag ataccgaatc
    11581 aagctgtcta ttaaatcccc ttgcccaaat tgttcttgat ctacagcgtg ctgcacaaat
    11641 tgtttagcat gctctgcaag cggtatgtaa tgacgtctta gtttctgggg aagtgattta
    11701 agaaggagtt gcaccttctc atcacacaga cccggtacca gccattctgc tttgagtgga
    11761 tcaacctgat ttaagacgct caagggtact atcagagtaa cgccatcttt aggactgccg
    11821 ggctcaaagt gataggcggt ctttagctca atgccgttca tcttgataac tttggggtaa
    11881 cgatctgccg taatcccagc agcctcatgg cgcatcagat cattcttttg taagcgcagt
    11941 gcgtgatctt tttgctcagt atcggatcga tcgcgttcta accaagcctg tagggtcgag
    12001 cgactataga tcgccttgtc gagttgttgg gcataaaagg cgaagagtaa ctcatcgtct
    12061 accaacacat cggggcggcg tgagcgatgc tcaagggcct ctatctcacg aatcagttgc
    12121 cggttatgcc agaagaatgc aaataatttg ccatagcgtt ttttggcttc ggcttgggtc
    12181 tctttctcaa gagcaggtgt atcgaccttg ccaaagagct cgccctcgac caatgccttt
    12241 tgtataaaca actgatgggc ctcgaggggg tcatgcggtg catacgcaac acggcggcca
    12301 tgatagatcg gtaagccata aagtgtggcg cgctcatagg ccattacttc gccactttgt
    12361 ttatcccaga agggatcgct cagtgtcttg atgagtcggt gcttggctac tttctcgacc
    12421 cactggggct cgatcttggc taaggttctt gcatacaaac gactggtctc ctgaatctca
    12481 ccagctacaa accatgcacc taccttttta ccaagactcg atccgggcca gagatgtaag
    12541 cggattcctc ttgcgcccat gtagttagca gtcttttgtg aacggctggt ggagtcttct
    12601 tcatcccgct tggcaatatg gcccaatagt cctgttaata atgccgtatg gacttgctca
    12661 taggtggcag gtgtttgatt gtctttccaa gccttttcag caagaagcac atgcagttga
    12721 ccatgcacat cacgccattc acgcaatcgt ctgggtgata aaaacttact acggcagagg
    12781 ttctcgagtt gtcgattgct ttgcttatgg gtcagcgcat cttgatacca gttccagagc
    12841 ttaatgaacc ccagaaactc ggagcgctca tcggcaaaga gctgatgggc ttggtctgcg
    12901 gcacttgcca tctccatcgg acgctcgcgg gggtcttggc aggccatcgc cgatgcaatg
    12961 atggtgagtt ccttgagcgc atgctgatcc
//
LOCUS       DDPQ01000069           15217 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_3645,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000069 DDPQ01000000
VERSION     DDPQ01000069.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15217)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_3645"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    7238..7342
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3853 a         3327 c         3707 g         4223 t
ORIGIN      
        1 tggattgctg tatttttctt aatcttttgt ttaccaagtc aacgatcggg tcgatattta
       61 ttaccgatca tgcccgcaat cgctatattg ttagcaattc attggcatca actcaatcga
      121 ctgctgttct gggttgcgct attgattcaa ggcatcctca ttattgcttt ggcatggctt
      181 agctggaata ttgatctatg gacctatcca gtatggcatt ggtttttact tcttggatcc
      241 atatgcgtca ttgggcttgg tttattccgc ctgtcactta ctaagtcagc cacattacta
      301 gcctgtttta tgagttatct atctttgacc agcagcgtta tgccgctgga tggatctctg
      361 ggacgtttta gcaaagcaac catccaaaaa cttcaaggca aaactatttg gtttccatgc
      421 gatttccgcg ccaaggatga ggaataccga ttactgattc ctggggctaa tatcaaaggc
      481 tatccagcgg gcgaggctaa ggagattaaa aagcttgcgg aacgttattc attgtttgcg
      541 gttcaagccc ccgtccaaag taagttagaa ctttgtgctg attgtgaaat catcggggag
      601 cggtttgaga tgcgcgcaag acataatgat gctgagatta aagctatgct catgggtcaa
      661 gtaagcgaca atctcttcgt gaaagagtac attgtttctg cgccatttaa tgctgccaaa
      721 gatataagta agttcaagga cgcctgtaga tgattagagt ctttgcattt atctttctcg
      781 ttgtggcact tgggatggcc tatctacaga tcaatgacca cccagcgtgg gctgattttg
      841 ttggtccgat cgatcaatcc gtaaatcctg atggacaaca gacaagtatt gatccacctg
      901 gagtggttac taagaaatta acaaccaaac caacctcagt caagctgcaa ggccaagttg
      961 actggcttcc agataccggt agaccctcag tgcatgccgc gtcattgatt ccgttaaagg
     1021 atggcaactg gcgcgcgttt tggtttgcgg gctcgcgcga gggcgctgcg gatgttgtga
     1081 ttcagagcgc ggtgtgggat gctaatgcaa agcagtgggg gcagagcaca gtcgcattag
     1141 atcgcgaggg cgcgcaacaa gggcttggcc gatatatcgc caaattaggc aatcccgtcc
     1201 cgattcgtaa tatcaatggc caattgcaac tctattttgt agcagtctca attggtggtt
     1261 gggctggtag ctcgattagt atgatgtact ccgatgatga ggggctgagt tggtcaagac
     1321 cgcaacgctt ggttactagc ccgcttctta atctcagcac cttggtgaag ggctctggct
     1381 tcttgtttag tgatggcagt atggggtttc cggtctatca cgaatggatc ggaaagtatg
     1441 gagagcttct gcgacttgat ccatctggac acgtactaga taaaagacgt atgagctcgg
     1501 ggcggggcac tcttcagccc attgtgtttg tggatgatgc aaaacatgcg agtgcttatt
     1561 tcaggcaggc acgcagtagc ggcgcaaagc agatcgctgt cagtcatacg gctgatgcag
     1621 gccgcctatg ggaaacggcc cctgatctac caatccccaa tccaaatgct gctattactg
     1681 gaattgaatt agccgatcgc actcgtttga tggtctttaa cgatctcgag catgggcgcc
     1741 atcgcttagt actagctgcg tcttggccaa agtctaataa tgaaaatagc agtcaaacgg
     1801 caaaagaaaa ttattctccc tggaaaacaa ttcaagtatt ggaagatgag actgcttcgc
     1861 aaagtaatcc aaaagaagag ttttcatatc cctatatgag catgaataaa caaggacagg
     1921 tatttttagt ctacacctgg aatcgcaaac ggattaaatc gattacatgg gaacctaata
     1981 atctagttgc ctatttgcat caagttcatc aagagatagt ttctgtcatt gataccccaa
     2041 cagaaatgaa ggaggccaaa tgaacttagc catgcttaca agtcaaattc ctttgattga
     2101 gttcggtgtc attactagtt ttgtgctgat gcattttgcc aggacttttt taggcttaag
     2161 tcaaagcagt attgccggca cattctttat tgtggtttgc accgtattgc tattgttgcc
     2221 atttaatagc ctaggcgctc catggaactt gcccttagta gcctatgttc gcggtgtgac
     2281 cggtgagctc agcgtgatta cgatgatgtt gatcctgttt acgtggtcat cttggccaag
     2341 ttgtcgccta tcaaatacga cccccattgt tattgcgctc ttgagtattt tgttttatcc
     2401 gttcgctttg ggattcacta tgtttgatcc ttatggctgg ggatatggat cggccatttt
     2461 tgtatccata gtgatggttg tagcgctcgt attgtttttt gctaagcagt cttgggaagc
     2521 tttgataatc tcgttggctg tgattgcatg gtcaattcat tggcatgagt ccaataatct
     2581 ttgggactac ctgctggatc catttttagc aatttgggca attacgctga gtattcgttg
     2641 gattgtttgg ggtcgaccag agccccatac ttactctgcc tgggatattg actcgagtgt
     2701 cctagctcga agaaaacagg gcaagatttt tagttaacag agagtaatgg tggtaaagaa
     2761 aaaggccggt taaaaaaccg gccttttcat tcagacggaa agcaagggat taatcaggga
     2821 tatttgcttt acgaatgatg gggccccagc ggttgatctc tgcatctaga tgtgcttgta
     2881 gtgctggacc attctgtttg gcaacgggaa caatatcaat gttcgaagag tctaagcggg
     2941 ccttaacatc tggatcagct aatgctttct tcaaagcagc attgatttta tccaacactg
     3001 gctttggagt tccttttgga gcataaacac catgccaaac cttcacgtca aagcctttta
     3061 agccttgctc atccaatgta ggtacattgg ggagcgctga taaacgctta ggagtagtca
     3121 ctccatagac cttaacgcgg ttatctttaa tgtaaggaat ggtttgtgtg gtttggtcgc
     3181 acaggatgtc tacttggccg cccaacagat ctgttaaagc aggaccagta cctttatagg
     3241 gaacggttgt tacacgcaca cccaatttgc tttggaaaat caagccgcat aactgagata
     3301 cagcaccagg accggcattg gccatggtta ccttctcttt attggtcttg atatatgctt
     3361 caagttcttt aaagttatta ggcggcaagt ttttgcgtcc caatagaacc attggtacgt
     3421 ctgctacttg accaatatat tcaaagtctt tcatggggtc ataaggcaac ttatcgtaga
     3481 gagcttgcgc agttgccata cccatgtgat ggatgtaaat catataacca tcaggcttgg
     3541 ctttggcaac cttgttggaa gcaaccgttc cgcctgcacc gggagcattc tcaacaacaa
     3601 ctgtttgacc aaggcttttg cccattggta ctgcaattaa gcgagcaatc gtatccgttg
     3661 gtccgcctgc agcaaatgga acaaccaggg taataggttt gtctggatat ttttgcgcat
     3721 tcgctgcgcc agctatcaca aggcctgtac caaggacggc agcaagcatt gtgtttaagg
     3781 attttttcag gttcattcaa agtctccaaa aggggtaaaa cgaggcttat tttgccatgt
     3841 ttttaaagga attagcttag ggattaccag ctaataccaa gattctgcgg gcaagcatca
     3901 ccacgggacg atcaaccatt tttccatcca ctttgacagc ctgtcctttg gatgctttat
     3961 ctgcagaaat aacccgcttt gcccagtcaa gctcctcggg gcttggagta aaagccctct
     4021 tcactattgg gatttgtttt ggatgaatgc atagctttgc cccaaaacca aaacgcttcg
     4081 ccttttgtgc atgtgcaaaa attcgtgctt catcatcagt agagggtgta accccatcaa
     4141 ttggaggggc aatttgtgcc aacttagagg ccagcaccag catgaagcgc gccgtatcaa
     4201 tttcagtttc attttcatca caactcatac ccatctcaac ttgcatgtcg agatttccga
     4261 gagcaaggcg taaaacttgt tctgcgctgg cgatttcatt gagatgatgc aggccgaggg
     4321 gtgtctcgat catggggatg aacgcagtat tttcgagttc ttcagcagcc ccattaattt
     4381 gatcgtgcga ttctgttttt ggaatcatta agtactgaac ctgtaattct ttagcaagca
     4441 caagatcagc agcataaaag ggggtgcctg gagcattgca gcgaatgatc atccgctgtt
     4501 tttgttctac agaaatcgat gaccatgcat ttttgaggag ctcacgtgct tttgctttgt
     4561 catctaaacc aaccgcatct tcaaggtcaa tgattactcc atcagctcca ctcgccagag
     4621 ctttgccata gcgctcgggt tgggtagctg gcacaaacaa aaaactacat gcatcgctta
     4681 agggggagtg gggtaagttc ataaggatgc tcctgcttgg ggaaggatga acggaatgat
     4741 tttggcctga cggatattaa atagtagtgt gatggcatct ttcatttcaa tttcggtagc
     4801 gccccgacta aatgcggcaa gccgtaatgc tttttcttgg atctcttcgc gacttagagt
     4861 atttcctgga tctcctttgg gttcgtctat acgaccgtca agcgtcttgc cgttcttgag
     4921 gtgaactttg accttaccaa tccagcgctg tgggtaggcc tgatccactt ccgtatcgag
     4981 agtcatggtc acgcgatcgc gtagggcaca aatttctgaa tcgtgaaagt ggtcagcaaa
     5041 ttcttgaaga ccagcaaagt gatacttgca gacaagggcg agtacggtac ccatcgagaa
     5101 cttcgattga tgcaaggtgc tcggtttgct ggcgggacct agtacatcga tagcaccctg
     5161 atgcacgaag gtctcaatgc gctcaatctc ctgtggctga acactatttt tctgcaaaac
     5221 ttgtaagagt gcatcggcag cagggtgggt gtgacgacag gaggcgtggt atttaaatga
     5281 agtctctgca atacaccaac gtgatcctag acgatcaatt aacttttctg ggttagcatc
     5341 gcttgacatg cctgcagcta aaccttgtgc cccctccagt atttttttag cgcccgtgaa
     5401 tcccgccttc gctagatagg ccgacatgaa tccagtagcg ctagcatgtg ctgtgtgcaa
     5461 ttgttttgag tcagccgcgt cgcgtaagaa ctcccaaagc cctgcagatt gtgttcctgc
     5521 agaaccgaag gcgtgtagca tctgctcggg gtttaagttc agcagacgcc caacggtagc
     5581 cgctgctgcc aaggtacccg cagtgcccgt tgtatgaaag attttgtagt gcgagcgccc
     5641 cagaaattct ccaacccgaa tacccacctc gtacccaaca acggccgcag ttatgagttc
     5701 tttgccgctg agttgattag cttgtgcgca ggctagagct gcagggaaca ctatcgttcc
     5761 tggatggaaa acggagccgt tatgaacgtc atcttgctca accacatgag atgctgccgc
     5821 attggctaaa catgccaaga atggactgga acttgttcgt gttactagga tttcagcaga
     5881 gcctgccaaa gcagtttgct ggtgtggacc catcgactgt gcaaattgcg ccatgatctc
     5941 cacaggcgtt gcacctttac cgccaattgc cgatgcaaac caatcaacaa acaaatcttc
     6001 agtacgagca atgactgagt gcggaatgtc atcgagcttg agttctgcag caaattgggc
     6061 aagttctgcg gagtaatgag tgctcattga atttagacca gctctgcttt ggcttgcatg
     6121 gttaaccaac cctcatgatc ttgtgcccaa accgaaagag ttttgccgct aggatctaca
     6181 gcaagatctg ggctggcgtt tacctgcatg gaatgaatat caaaggtcgg tcgaacggct
     6241 cggaactcaa accgctttaa tttcttccca gggttgctct gccgcacaag atccactaat
     6301 agagtagcaa tgaggggacc atgcacgatc aaaccgggat aaccttcttc ttgggtcaca
     6361 taactgcgat cgtaatgaat gcgatgccca ttaaacgtta aggctgaata ccgaaataac
     6421 aatactggat cggggtggat gatcttgctc cacttggcat cacttggagc tggagttgga
     6481 tcaggagcct tttcatcggg agagggtgcg gcccgataca caatgtcatg ctcttcagtg
     6541 agtgcgaggc ctttggaatt atgaatttga tgttctacta atacaaacag tagatcacca
     6601 cttcgtcctg atttatgggt cactgattta atggttgaga cacgctcgat ggcatcgcca
     6661 ataacaagat cctgttccca gcgaagtcga ccacctgccc acattcttcg tggtagagga
     6721 atggggggta gaaagccacc gcgtttgggg tggccatcgg gtccaatttc agactgtttg
     6781 gcatggggca agaaatacaa ccaatgccat aactcgggaa gaaacactcc attaatggga
     6841 tctggatcct tacggtcgag ggtggctgat aaggcgcggt agggggcgat agtgaccgtg
     6901 tcttgatatc gctcggtttt accaatccat tcttggagat gggctagtgt cggtgggtcg
     6961 attctcatac tctcattatg ccaatcctgg cagcgctacc atccaaagta aattattgga
     7021 atagaagtcc gctgggtgca aaaagaagtc ctgctaaagc agctagggct agggttcgga
     7081 tgactcccca ctgatatcga atcatcagca agagggcaac tccgcaaatc aacagtgata
     7141 tccaagaaat ccccgcagag aggccgctag gaaaaaacac atggtacgca aagaaaaccc
     7201 ctagattagc aattacgcca acgactgctg caccgatnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nntaacctac aaacgcaaca atcataatga gtggtcccgg
     7381 cgtcgtttca cccaaagcta gaccatcaat catttgggtg gcagagagcc attgatagtg
     7441 ctcaacagcg ccttggtaga cgtagggcaa tacagcataa gcccccccaa aggtaagcag
     7501 tgctgcttta gtaaagaacc aagccagttg tggatacaga gttttccagc ccagcagaaa
     7561 gataaggcat gctattggca caaaccacaa gaggctagca atcgtaattt gccagatgag
     7621 ccgcgctcga ataaaaccgg catgacttgg aggcggcgta tggtcatcaa tgattgcatt
     7681 ctcatgatgc ttgggtaatt gactgaaatg cgttttttgt tcttgagaaa aaagttcagg
     7741 atggcgttgg ccagcccata agccaaaaat acctgcgcta agaatgatga gtggaaacgg
     7801 taggttaaac acaaagattg caataaagga gagcagcgcc aatattgcta aagtacgatt
     7861 ctggatacta cgtttagaaa tacgaatcac agcggtaatg acaattgcgg ttactgcagg
     7921 cttaattcca taaaagacgg cggtcactat ggttgtttgg ccgtagtgaa catatagcca
     7981 cgaaagcgca atcaaaataa ataaagaagg cagaacaaac agagcgccgg cgagaatgcc
     8041 gccccaggtg cgatgcatta accagcctag ataggtggcc aactgctgag cctctggccc
     8101 cggtagtaac atgcaatagt tgagggcgtg caagaaacgt cgttctgaga tccaacgttt
     8161 tttatcaacg agctcgtcat gcatcatggc aatctgagca gtgggccctc caaaactagt
     8221 aaagccaagc ttgacccaaa aagcgagagc atctcgcaag gggatgggtg atagttctgt
     8281 cgaagaacta gttggcttga tttttaaacc tcatccatcc cgccaacaac ttggctaaaa
     8341 ccgttatcga catagatgat ttctgcggta ataccatttg ccaggtccga taaaagaaag
     8401 gatgcagcat taccaacatc atcaacgcta acgttgcgac gcaaaggagc ggtcttttca
     8461 accgcatcca atatcttgcc gaagcctttg atgccagaag ccgcaagagt tttaatgggg
     8521 cctgcagaaa taccattcac ccgaattcct ttagggccta gagagccagc taaataacga
     8581 accgatgctt ccagggatgc cttggccaac cccatggtgt tgtagttcgg aacattgcgc
     8641 attgaaccaa ggtaggtcaa agtgagcaag gcagcattgg gtcgtaacat gggcattgcc
     8701 tcttttgcca tagctgggaa actataggca gagatatcgt gagcaatctt gaatgcttca
     8761 cgactgagac cctctagaaa atctccggca atcgcctctc ggggtgcaaa gccgatggaa
     8821 tgaacgaaac catcaaattg gggccagctt ttggcgagat ccttaaatag agccgtaatt
     8881 tgttcatcac tacccacatc gcaatcaaaa atcagctggc tattgaactc tttggcaaac
     8941 tccgtaattc gctctttgaa gcgctcacca acataggtaa aggctaactc agctccttca
     9001 cgatggcatg ctttagcaat accataggca attgagcggt ttgaaagaag tccggtaatg
     9061 aggatgcgtt tgccgcttaa ataagccatc ttgagtcctt tactccaaaa ttgatttgct
     9121 atctacaatt gttgcatatg ctaagccaaa ttagacctaa tcctgtaggg atgccattta
     9181 gggcccaggt tctccttatt gctcttggac tcttatgtgg cctagggttt tattcgggag
     9241 aaagtgctgc tgcccaaggg attgctcagt atgggaagcc caaatatgcc gaaggctttg
     9301 ctcattttga atacgccaat ccaaatgctc ctaaaggcgg caccctaacc ctacccaacc
     9361 cagatcggcg aaccagtttt gataaattca atccgtttac tcttcgaggg gtaacggcgc
     9421 ctggtattgg tgccttaatg tttgagtcct tggcagtagg tagtgccgat gaagtatcta
     9481 gtgtttatgg cttgattgcc gaagatattc aagtagcacc tgacaaaatg tcagtgagtt
     9541 ttcggatccg acctgaggcg cagttctcgg atggctcacc tatattggct agtgatgtta
     9601 aacatagttt tgacacctta atgagtaagc tggcaaatcc acaatataga actatctatg
     9661 cagatgttaa acaagcggta gttgtttctg aacgggtgat acgttttgat ttcaagactc
     9721 gtaatagcga actaccgttg atggtgggtg gcctgccaat cttctccaag aactggggtg
     9781 ttaaaccaga cggtagtgtt actgcctttg acaaaattac ttttgagcat ccaattggca
     9841 gtggccctta cgtcatcgag tcctttcgtg ctggtaaatc aatgatctat aaacgtaatc
     9901 caaattattg gggtaacaag gtcaatgtgc gggtcggctt ttataacttt gatcggattg
     9961 tttataagtt atacagcgat gatgcggtgc gtctcgaagc atttaaggcg ggggagtttg
    10021 atgccttagt cgagtaccgt gccaagaact gggcaaagag ttatgtgggc ccaaagttta
    10081 ataatggcac tctcattaag aaagccttcg ccaatcataa tggtgccggt atgcagggtt
    10141 ttgccatgaa cctacgtcgt cccatttttc aggatgtccg tgtgcgcaaa gccctgggtt
    10201 acgccctgga ttttcagtgg ttaaatcgac aactcttttt tgatcaatat ggacgtataa
    10261 acagttatta caccaatagt gatttgagtg cgaactaccg aggcgaaacg gtcccgacag
    10321 aagccgaact caagctatta aaaccattac aagcgaagta tcctcaacat gttcctgagg
    10381 cagtctttgg ggcaatgccc ccaccgccca gtacagaagc cccgagcagc ttacggaaca
    10441 atttacgcaa agcccgtgag ttgttagcag aagcggggtg gacctatcgc gatggagcac
    10501 tgcgaaactc gaaaggagaa atctttcgtt tcgagattgt ggaggatggc cccttcttca
    10561 tgcggattct ttctgcctat gtgcgtaact tagaaaagtt aggcatcaaa gttgagatac
    10621 gaactagtga ctatgctttg caccagaaac ggatggatga ttatgatttt gatatgacca
    10681 ccatacgctt ccccgatacg caaagtcccg gcaatgaact atatgaccga tttgggagtg
    10741 cagctgccaa agagagagga tcggataatg tcattggtgt gcaatcccca gtcgtggatg
    10801 ccctgattga tgcgatcgta aaagccgaga cccgtgagca gttacaagca gctacccgtt
    10861 cattagatcg agtgttatgg aacagttact tcgtagttcc tcattggtat aacccgacgc
    10921 atcgtatcgc atttcgtaag gagatgggct accccaatcc tcctttgtat tactccgctg
    10981 aatcatggat cttatccacc tggtggttac aagaggcacg taaatgaact cagagctact
    11041 tagttatatt ctgaagcgta ttttgttgat gatcccgact ttgctggggg tgctgaccct
    11101 aacctttgct gtggtgcaat ttgttcccgg cggtcctgta gagcagttaa tgctagagct
    11161 tcggggaaaa ggagatgccg cagtcggagg tgagtcatca ggtggaggaa ccacctatcg
    11221 cggtcggcaa ggcttggatg cggagcgtct agagcaagtt aaggctttat atggttttga
    11281 taagccggcc ctggagcgtt actttgcgat gcttaaacga tttgctcagt ttgatctggg
    11341 ccaaagttac taccaacaca agagtgtgtg ggatctggtg atctctaaat taccagtatc
    11401 tatcagcatt ggtttgtgga ccttctttat tagttacttg atatcgatcc ctttgggtat
    11461 ttctaaggcg gtacgcgatg gtacgcgttt tgaccgagtt accagcacca tgattttggt
    11521 tggctatgca atccccggat ttgttttagg agttttattg cttgtattgt ttggtggcgg
    11581 cagtttttta cagatctttc cgttgcgggg tttaacttcc gataactgga atgagctcag
    11641 catgatgggt aaggtcttag attatttatg gcatctagta ttgcctatta cagcatcggt
    11701 tctcggaagc tttgcagtga ttaccatgct caccaaaaac tcttttttgg aagagatccg
    11761 caagcaatat gtcttaacgg caagagcaaa aggggtaagt gagaataagg ttctttggaa
    11821 gcatgttttt cgtaatgcac ttatcccctt aattactgga ttcccagcag catttattgg
    11881 cgcattcttt gcaggttcat tgctcattga gaccttgttt tcattggatg gccttggctt
    11941 gctgtcgtat gaggcggtca tgaagcggga ttatccggtg gtatttggca ctctttattt
    12001 atttacctta attggtttgg tcacaaaact aatttctgat ctttgttatg tcttggttga
    12061 tccacggata cagtttggtt caggaggtgg ctcgtgagtc gctggtcacg ctttaagtcc
    12121 aatcgcttag gctactggag tctgtggatt ttttcgattc tcttcggact tagtctttgt
    12181 gcagaactca ttgcgaatga caaacctctg gtagtgcgct atgagggcca gttttatttc
    12241 cccatgattc agaatcatcc agaaactgtc tttggaggag actttgccac tccaaccgat
    12301 ttcttagatc ccgatatcaa gaaaaatatt acacaaggtt ctaattgggc tatttaccca
    12361 cccatcccgt atagctttca gaccttaaat tatttctcca aggagccaaa tcctgcatcg
    12421 ccttcattgg ataactggtt aggcacagac gatcgagggc gcgatgtttt atctcgctta
    12481 atttatggct ttcgtctctc tattttattt ggcattgcct taacgattgt gggagtcgtg
    12541 gttggggtct tgactggcgc actgatggga ttctttgggg ggcgctttga tctgattacg
    12601 caacgcatca ttgagatttg gtcagcgatg cctgagttat acctattgat tatttttgcc
    12661 tcgatcttta atcccagcgt tcttctattg attattttgt tagcagcatt tggatggatg
    12721 ggtctatcgg actatgtgcg agcagagttc tttcggaatc gtgccttgga gtatgtgcgg
    12781 gctgctaaag cattaggcct cacaaatgcg caaatcatgt ggcgtcatat tttgcccaat
    12841 agcttgaccc cagtcattac attcttacca tttcggatga gcgcagctat cttatcccta
    12901 accagcctag actttttggg attgggcgta cctcctggta ccccgagctt gggcgagctt
    12961 ttgtcacaag gtaaggccaa tttagatgcc tggtggattt ctttatcaac ttttgtggtg
    13021 ctgggcggca cattactctt gctcaccttt atgggcgaag ccttgcgcga tgcatttgac
    13081 tcccggaaga gcaatccgct gactggagtg aaagcatgag caccagtcta ttacgttatg
    13141 agaacttctc actctcgttt ggtacaggtc gtcgtgagaa atttgcagtt cgtgagctct
    13201 cgctggagat tgacgtgggt gagcgcatcg ccttagtggg cgaatcgggc tctggcaaaa
    13261 cattaaccgc tctctcacca ttgcgtttac agcctgaggg agcgcacctt actggcaaaa
    13321 tttattggcg cagggcggat ggtacagagg tcaatctttt ggatttaagt ctggagcaaa
    13381 ttcgggagat acgggggcgc gagatcgcca tgatttttca ggannccgat gaccgcatta
    13441 aatccattgt ttaccattgg caatcagatt gtcgaagcgg ttcaagtgca tgagccttta
    13501 ttgccacttg ccgaatgtcg caatacagcc gttgcattgc tcaagaaaac gggtatacca
    13561 gaaccagaag agcgttttca tgcctatcct catcaactat caggagggca acgacagcgc
    13621 gccatgatcg ccatggcatt ggcatgtaag ccacgattat tgattgccga tgaaccaacc
    13681 accgccttgg atgtgagctt gcgtttacag attttggatt tgttaaaaga cttacaagaa
    13741 gaggctaaag agcggggagg gatggcgatt cttttaatta cccatgatct taatttagtg
    13801 agacactttg cccatcgtgt gggtgttttg aatcagggtt gcttggtaga aagtggctct
    13861 acccatgagg tatttgcaca ccctaaaaat aattacacgg agcaattggt taatagtcag
    13921 cctgtgaggg agctgttgcc cctagtgccc ctagcgccgg tgctcctgaa agtaaatgat
    13981 ttaagcgttt cttatcccca gtctaatttc tcgggagcgg gtctgcgcat tgcgaaatgg
    14041 tttaccaaag caaaacgcaa taccgtattg caacccttga gcttccaact aaagcaaggt
    14101 caaaccatcg gtgtaattgg tgaatctgga tctggcaaaa ccaccttagc aatggcaatc
    14161 ttgggtctac tgggcgagac caccgcacgc cttgctggtg atgtggcagt tttggaccaa
    14221 tcgtggcagg caatgtcacc gcttgctaga agagcaatgc gggcgaactt acaggttatt
    14281 tttcaggatc catttggttc tttatcgccc cgtatgacca tctttgatat tgtttcagag
    14341 ggattaaata ttcatcaacc aacgctatcg actgtggagc gtgaggcgcg tgtcattgag
    14401 atcttgcgtg aagtgggtct ggatcgttca gtgatgatgc gctatccaca tgagttctcg
    14461 ggtggccaac gacagcgtat tgcaattgct cgcgctctga tattaaaacc acagattttg
    14521 gtcttggacg aaccaagctc ggccttggac gtgtcgattc aaaaacaggt cttagcattg
    14581 ctcagcgctc tccagaaaaa gtacaacttg gcctacttaa tcattagcca tgatctggcg
    14641 gtaatccgtg cgatgtctca tgaactgatg gtcttgcgcg gtggggaggt tgttgaatac
    14701 ggtgagaccg aggcgatgat tgcgagccct aaagagcctt ataccaaggc cttatttgcc
    14761 gctgcagagc tggtatagat ctaaattcct ataaaaaaca gttacttaga ctataaatct
    14821 cggcaatacc gctttctaaa tcggattagc tttgttagaa ttgagctatg tctttgaaaa
    14881 agcaagcgat ctctttgtct ttaaagtcct tgaccgttct ggttttgggc ttcgcttcgc
    14941 ctttgctttc tgccaatact gctgaagagg caagcaataa agaaggtact aaagttgaga
    15001 gcattcttga gagtacctct tcatactttg ctcgtttcac ttatcaagta gttgattcgg
    15061 tatcaagcaa aacagaatct ttgattaacg aggcaatgca acttattgga gttcgttatc
    15121 gctggaatgg tgatttacct caatcgggtt tagatgccag tggctttgtc agatttgtgt
    15181 tcagagataa gctaggtttt ttattgcccc ctaagtc
//
LOCUS       DDPQ01000070            5632 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_16478,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000070 DDPQ01000000
VERSION     DDPQ01000070.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_16478"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3670..3684
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1468 a         1202 c         1447 g         1500 t
ORIGIN      
        1 ctagcctcac gtttatcgtg ttgcttctta ccccgagcta aaccgatttc gcatttcact
       61 cgtccttttg agaaatgcat attgagtggc actaaggtat agccacgttg ttctacttta
      121 ccgattaact tacgaatctc gctctcgttt aagaggagct tgcgcgttcg agtaggatct
      181 gcatgaatat gggttgaggt cgaggagagt ggactgatgt gcgagccgat taaaaagatc
      241 tctgcattac gcaccacgac ataagcctct ttgatctgag cgcgacccgc acgaatggcc
      301 ttaacctccc acccctcaag gacgagccca gcttcgtagc gttcttcaat aaaatagtcg
      361 aagaaggctt ttttgttatc gacgatactc atgttttaga gtttttatag gttccatcgt
      421 attgattatg gcagacgtca ataagaccgt tttaattggg cactccgccg agcgcatgta
      481 cagcttagtt acggatgtgg cgagctaccc agatttttta ccctggtgcg gcggtgtgga
      541 tatctacgag caaaccgaga ccgtgctgga cgccaaaatt aagattcact tcaaaggaat
      601 tgagcagtat tttcatactc gtaacgccaa tcatcgaccg gatttaattg atatgcaatt
      661 tgtcgatggc ccctttaaga gtttcactgg gcagtggcgt ttcattgcat tgcgtgagga
      721 cgcttgtaag atcgagttca atcttcattg ggagtttaag aacatcattc ttgataaagt
      781 gatcggccct gtgttttctt atatagcagg cacctttgtg gattgttttg taaaacgagc
      841 ggatcaactc tatggccact aatacctcca tcaccattga gattcttctg tgcgatgctc
      901 gggtagaccc acccaaacta caaacacatc aacttcattt gcccaaggaa ggatctccaa
      961 cggtaggctg ggctcttcaa tctctacaaa ttgctcacgc caaggatgat ccggtaataa
     1021 gccgtaaggg ttgctatggc gtatttggta aacgcaaaga gtgggatagt cccatctatg
     1081 cgggcgatcg ccttgagttg tacgcaccct tacaaattga ccccaaaatg gcccgtcgca
     1141 agaaagccaa ccgagataaa gatagtcaat taaaagccaa ggcacaagat cgagccagcg
     1201 cacgaacggc gaaagcaaaa gcctctaaat aggcttaaaa acccgactat ttgagtgcta
     1261 aggccctgat caggaaggga tttaagagtt aattctggag aacctctata atctgatttt
     1321 gcgactaggg gttttgcatg cgactcatac aaaaagcact gacgttcgac gatgtgctcc
     1381 tcgtgccggc atactcggcc atcctcccgc gtgacactaa tttggtcacc caattaaccc
     1441 gcgacatcac gatcaatatt ccattggtat cggctgcaat ggataccgtt acggaaggac
     1501 gtctagcgat tgccatggca accgagggcg gcgttgggat tgttcataaa aaccttaagc
     1561 ctgcggaaca ggctaaagag gttgccaaag taaagcgcta tgagtctggg gttctgcgcg
     1621 atccaatctc aatcggccca gatatgacgg ttcgccaagt tattcactta tctaaagagc
     1681 atgggttctc tggatttccg gtgttacaag gtaaaactgt agtgggcatt attacaaacc
     1741 gcgatctgcg ctttgaagaa gatcttgatg cacctgtgaa agccaagatg accccacgtg
     1801 aacgtttggt tactgtcaaa gagggtgcta gcttagaaga agcaaaacgc ttaatgaata
     1861 agcatcgcct agagcgggtg cttgttgtga acgactcctt tgaacttcgt ggcctagtaa
     1921 cggttaaaga catcatgaag gctacagagc accctaatgc ttgtaaagat agtgaaggta
     1981 agttacgtgt cggtgcagca gttggcgttg gtccggataa cgatgagcgg gttgagcttc
     2041 tggtgaaggc aggtgtcgat ctaatcgtgg tagacactgc ccacggtcat agtcaaggcg
     2101 ttcttgatcg agtgaagtgg gtcaagaaac actatcctca agtacaagtg attggcggta
     2161 atattgcaac aggcgaagct gctaaagctc tggcagatca tggtgctgat ggtgtaaaag
     2221 ttggtattgg tcccggatcc atttgcacaa cccgtatcgt tgcgggcgtt ggggtccctc
     2281 aaattactgc ggtcgtaaat gtggcaaagg ccttaaaggg gaccggtatt cccttgattg
     2341 ctgatggtgg tgtgcgttac tcgggcgatg ttgctaaggc tttagcggct ggagcaaact
     2401 cagtcatgat gggcggtatg tttgcgggta ccgaagaagc tcctggcgaa gtatttttat
     2461 accaaggccg ttcttacaag agttaccgag gtatgggctc attaggtgcg atgtcggatg
     2521 gtgccgccga tcgttatttc caagaagata ttagtgcagc caatgctgat aaattagttc
     2581 cagagggtat tgagggacag gttccttaca aaggaagtgt gctgagcatc ttgcatcaac
     2641 taactggtgg cattcgctcc tcaatgggct actgtggttg taagactatt tctgagttgc
     2701 atgagaaggc aagttttgtg gagattactt ctgctggagt gcgcgagtcg catgtccatg
     2761 atgtaaagat cacgaaggaa gcccccaatt accatattga ctaagaatcc aattgagcaa
     2821 agccgtgcac gataagattc tgattctgga ttttggttcc caagttactc aactaatcgc
     2881 tcgccgtgta cgagaggcta aggtttattc tgagatctgg ccatacgatt gtgatccaga
     2941 gcaaattaaa gagtttgtaa gaagtcagtc ttgcaagggg atcatcctct ctggtggccc
     3001 aagctcggtt acggagccaa atagccctcg cgccccacaa attgtctttg aactaggcgt
     3061 ccctgtattt ggtatctgct atggcatgca aaccatggca actcagcttg gtggcagggt
     3121 tgcctcggca gagtctcttg gtaaggcacg tgagtttggg tatgccgaag tgcgtgctcg
     3181 tggtcatacc gagttactca aagacgttga ggaccatcga actgaggaag gtcatggcat
     3241 tttgaaagtt tggatgagtc atggcgattc cgtgacagag ttgcctcccc agttcaagtt
     3301 aatggcttca actccctctt gtccaattgc gggaatggct aatgaggaga agcatttcta
     3361 tggcctccag tttcatcctg aggtgactca tacactgcag ggaaccgcga tcctcaatcg
     3421 cttcgttcat gacatttgtg aatgtcgccc tgattgggtg atgggagatt acattcaaga
     3481 agcggtggcc aagataaaag agcaggttgg cacagatgag gtgattctgg gcctatcggg
     3541 cggcgtagac tctagtgttg ctgcagcact tattcatcgg gctattggtc agcaattgac
     3601 ttgtgtattt gtcgatcatg ggctcttgcg ccttaatgaa ggcaagatgg ttatggatat
     3661 gtttgctcgn nnnnnnnnnn nnnnctcaag ggcgttaccg atccagaaca aaaacgtaag
     3721 atcattggta aagaatttgt ggaagtattt cagaaagagt ctgccaagat taagaatgcc
     3781 aagtggcttg cccagggaac gatttacccc gatgtcatag agtctgctgg caaaggtaag
     3841 aagggtgccc atacgattaa aagtcaccat aacgttggtg gacttccaga ggatatgcac
     3901 ctcaagctat tggagccgct tcgagagctg tttaaagacg aagtaagaac acttggtatt
     3961 gccttgggct tacctaaaga gatggttgat cgtcatccat tcccgggccc aggacttggg
     4021 gtcagaatat tgggtgaagt gaagccagag tttgcagatc ttctcaggca cgctgattct
     4081 atctttattg aagaacttag aaatactata gatccaaatt ctcataaaac ttggtatgag
     4141 cttaccagtc aagcctttgc cgtcttcctg cctgttaaat cagtgggtgt gatgggtgat
     4201 ggtcggacct atgagtatgt cgttgctcta cgagcagtac aaacccaaga ctttatgacc
     4261 gcccactggg cacatctacc tcatgagcta ttgggtaaag tatccaaccg catcattaat
     4321 gaagtgcgcg gtatcaatcg tgttgttttt gatatcagcg gtaaacctcc ctcgacgatc
     4381 gagtgggaat aaaaaattaa tttagatatt cattgttatg tactcagtta aagaactctt
     4441 tgctactctg caaggagagg gtgcgcaagc agggcgtgca gccgtatttt gtcgctttgc
     4501 tggctgtaat ctctggtcgg ggagagagga agatcgcgct actgccgttt gtcagttctg
     4561 tgacaccaat tttgttggaa cggatggtat aggtggtggg aaatttgaga gtgccgatga
     4621 tttagcaaaa gcgattgagt ctgcatggca agaggctatg aacacacagc actatcgcta
     4681 tgtggtgatg accggtggcg agcctttatt gcaattagat gcctcattga tagaagcctt
     4741 gcatcaacgg cattttgaaa tagcaattga gaccaatggc acgattaaga tccctaaagg
     4801 gattgactgg gtttgcttaa gccccaaagc caatgctgat ctgatggtaa tgcaggctga
     4861 tgaaatcaag ttagttgttc cgcagataga acatcaaccc attgaaacaa ctttgcaccg
     4921 ttttgagggt atggatttta ggcatcgtta tttacaaccc atggatggcc ctaatttaag
     4981 tgaaaatacg gcatatgccg ttgagttgtg ccaaaaaaat cccttatggc gcttaagtgt
     5041 gcagactcac aaaatgatcg gaattctgta atggtggacc aaaactctgt aacgattact
     5101 cggcgcctcg agtttgatgc aggacatcgt attccaaatc atgcaggcaa gtgctgtcat
     5161 ttgcatggcc atcgatatgc gattgagctt actgtgtctg gaccggtaca tgcatcgcgc
     5221 ggtcagtctg acgatggcat ggtgatcgat tttggagaca ttaagcaact agcactcaag
     5281 cacctggtag agccctggga tcatgccttt ctggtcgcta aacacgatac ggtcttggtg
     5341 acgttcttgg agagtttgcc tagtcacaaa acagtgatct tagaggatgt ccccaccgtt
     5401 gaaaacctgg ttaaattcgc ctttgatatt ttggctcaag tatttacaac tgagacaaag
     5461 gggcagatgg tcttacaggc aatccgttta tatgagaccc caaactgttg ggctgactat
     5521 cgagcaaaaa gcggccccaa aacttgatga ccagtaccca agaccaagct tatatgcgtc
     5581 ttgccatcga gcaggctaga ttggctggtg cccaaggaga ggtccccgta gg
//
LOCUS       DDPQ01000071            2565 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_43306,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000071 DDPQ01000000
VERSION     DDPQ01000071.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_43306"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    335..416
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1931..2010
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          587 a          547 c          532 g          737 t
ORIGIN      
        1 aaattacacc ggccagttat caattggttc ttaatcactt tcccattcct aagaatgagg
       61 tgttatttgt ttcgtgcaat gcctgggaca tcattggtgc caattggttt ggttttgata
      121 ccttctgggt taatcgctat cagttaccct ttgaagaaat tggcttagcc ccgacacatc
      181 aaggcaatga tttacatgat cttcgacaat tactactttc ttaagtaaag atcattgccc
      241 caatttcata gagtagataa gcaattccta tagctataac tacatagacc actgtgctaa
      301 caaagctttt gcccaaaatg ccaaaacgct ctagnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatt
      421 gcatccccgc attctagagc tttttcttat tggggtgaat gtcattacaa ttccacaaac
      481 actctgaaag cttatcgtgg attttgctta cctcatagat ttatttcttc atcttgataa
      541 aaatctagcc ctcattgcca atgaatgggg tacgtggatc tacgttctcc tctttgccat
      601 tatttttgtt gagaccggtt tggtggtcat gcctttttta ccaggcgact ctttgctctt
      661 tgttgccgga gccctcactg ccgtgggtga gctaaacctt ccgctcttaa tgtttctctt
      721 aaccagtgca gcgattcttg gggatgccgt caattactcg gttgggcgct atgttggtaa
      781 taaagtattt tcttgggaaa actcacgttg gtttaataag aaggcttttg accaagccca
      841 cgccttctac gaaaaacatg gtccgataac gattgtgatt gggcgttttc ttccctttat
      901 cagaaccttt gcccccttcg tagctggcgt ggctcaaatg cgctacccag tctttgtctt
      961 ttacaacatc attggcggaa ttatttgggt ctgcagtctc actgccttag gctatctgat
     1021 tggcgatcat ccttgggtta agagtaattt ttccttagtc gctctcgcga tgatcatcat
     1081 tccaggccta cccgctttat ggatctttat gaaagaattg ctcgcgcgct ttcgcaagta
     1141 agccccgcat ctatcgtatg aacactactc atcaccaaaa caaaccgatt gtcatcattg
     1201 gtagcggctt ggctgcctat accctgatcc gggaataccg caaactgaat gctgagcaag
     1261 ccattactgt aataactcaa gagagcgggg atttttattc caagcccatg ctatccactg
     1321 cttttgcttc taagaaagat gctcaacaac tgatctcttc aagtgcagca aaaatgatgg
     1381 agcaactcaa tatccagatt cattcacata gcaaggtttt gaagattgat gggtccgatc
     1441 aaagtcttag cttccaaagt gctgatggca aagaacatgt tttgcagtat tccaaattag
     1501 tcctagcctt gggggccgat caaatccata tagatctggc tggcgatgga gctgatcaag
     1561 ttatgaccgt caatgatttg caggattacg ctgtttttag agaacgcatt ggccagcaaa
     1621 agaagcttgt gatcttaggg gccggcttaa ttggttgtga gtttgccaac gacctcagtt
     1681 taggtggcta tcacgttgag gtcgttgatt tagccgatca agtgttagga cgccttctac
     1741 caagagtaat tgctgatgag cttcaagcaa agttatccgc tctgggggta ctctggcatc
     1801 ttggcaccag tgcaaagact gtttcaagag taggagatgg gtttgagatt ggtcttagca
     1861 atggaaatgt tttgcaagca gattgcgttc tctctgcaat tggtcttcgc cccagaactg
     1921 aattagccaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggaatatata gtcttggcga ttgtgccgaa
     2041 gttgaaggcc atgtcttgcc ttacgtaatg cccatcatgc aggctgctcg tacactagca
     2101 agtattctgg cagggcacga tagcgcccta atctaccctg ccatgccggt gatgattaag
     2161 actccagctt tacctctggt ggtatctccg cctgcatctg gtagcgaggg ctcatggacg
     2221 attaaacaag aagagaatgg cattgaggga ttgttctata gcccccaagc ccaattactt
     2281 ggttttgctt tagcgggatc cgcaacagcg cagcgcgcta ccctcaccaa gctcttgccc
     2341 aatattttgg gcgagtaagc tcaagtaact agcaacgtac tgaggagacc aatcaatgcg
     2401 catgatctta tatctgatgg tggctttaat acctattgcg attgtttact cgcgcttttt
     2461 ggatggtagt aagctcaaag agtttttggc ggctgtctcc tacttgagcg tgatggcttg
     2521 ggcatttttc tttgccctca ttcttgaaat gctcttacgc atccc
//
LOCUS       DDPQ01000072            4977 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_17839,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000072 DDPQ01000000
VERSION     DDPQ01000072.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_17839"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1211..1261
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3321..3381
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1341 a         1162 c         1071 g         1289 t
ORIGIN      
        1 gcattaggga agaaggactg ctcaccagca acgcggtagg atgcgatgct atcttgacct
       61 tctttggaga taatccaatc aataaatgct tggccttctg ccttcttcac ctttgggtgc
      121 ttcgcagaac tcacgagcat cacgccatat tgattgaaga gttttgggtc gccctgaacc
      181 agaatggtga gatcaccacg gttcttaaag gtcagccagg tagcgcgatc tgacaagata
      241 tatccattca tgccagatgc agtattgagc gccggaccca ttccagaacc agtctctttg
      301 taccaaggca tattggcaga cacttctaca ccagcgccct tccaataacg caactcagca
      361 gcatgggtgc cactcttgtc accgcgtgat ataaaacttg ctttgctatc tgcaattttc
      421 ttgagagccg cttgaatatc tttaccacca gcaaccttgg ccggatcgct cttaggtcca
      481 agaagtatga agtcgttgta catcacttca atgcgcttat cagcaaaacc atcggcaacg
      541 aacttctctt cggccacttt gtcatgaaca aaaacaacgt cagcgtcacc acgacgacca
      601 atatcaagag cctgaccagt gcctacggcg accaccttga cctgaatccc tgtttttttc
      661 tcaaagggtg gcaagatatg cccaaagagt cctgactgtt cagtcgatgt ggttgaagag
      721 agaacaatac tcttttgagc ataggcctga cctagaaggg tggctgataa caaagatgct
      781 agaagtaatt tgtgtagaat tttcatgatg gaaatgtgct tgagttaaaa ccacgtatca
      841 tgcataaatg atgtgattta atcaatatgc aaaaatattc atatgaagct agtcatccaa
      901 cccaccctac tccttaaagc ccctaactan ncatcagcaa agtcttgacc ttagccatat
      961 tggcaatcta ctaaaaagtg tcgagcaagg tcgcaccctt gcgtctgctg ccaaagacct
     1021 taatatttcg tatcgcactg tatggaatga gctcaaagag gcagagcaag aactgggctg
     1081 tcgatttctg gatcgggtca aaggccatgg ctccaaatta acccctgctg gcagattact
     1141 gattcattcg ctcgatagac ttgatcttca gctatcggat gaattgcaac gccttgccga
     1201 tgatatcggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nagcagtaat gaccccttaa ttgaatacat tagccccaag tttgaggaga tcaccttcga
     1321 gactatgggc tcgggtcagg ccttagatcg attgctggaa gataaagcca atattgcagg
     1381 ttttcatata tcggacccgc aagaaattaa gggtatccag gatcatttac aacgccgtgg
     1441 cctcgttgcc attcccttaa tgcaacgcat tcaagggctc attcttgcaa agggcaatcc
     1501 tttgaagatt aaatcggtac gcgatctagc aagaccagag gtgcgcttta tcaatcgtca
     1561 aaaaggtgct ggtacccgtt tattgttaga ccgactgatt gaacgcgaag gactcaagac
     1621 tagacaaatt aagggctatg ctcatgaaga atttacccac aatgccattg cgacaagtat
     1681 tttggctggg caagcggacg ctgggatggg actgcaatac attgctaaag aatttagcct
     1741 aagctgtatc ccacttgaaa cagaaacgtt ttatctagcc atgaagcctg agctcagaaa
     1801 aaacaagacc atcgttagtt ttataaagag tattcagaac cgctcaaaca aaacagccgg
     1861 ctataaagca ttaacctaag tcacttaata cacaaatgga attaacttct tagtcgttat
     1921 tttgtactcg gcatactcag aaaacttttc tgttaaccag atctcttctt gtctggattt
     1981 aagatcaaag aagattagca agatgatggc aagcaccaag gtgtagaggg tttgctctat
     2041 tccagcccac ccaaagcaca ctaagatcac cccaaaataa atggggtgcc ttacaaatcg
     2101 atatagccca gtccgaatta gctcaccatt ctgtttgggt ttgggtagcg gtgtcaaatt
     2161 aggtccgagt aagattgccg cccaaattgc gatggcaata cccaagtaga agagagtttg
     2221 accaatccac cataaggttt gattgacctt ggttggcaac cccaatagat cctttggccc
     2281 aaagaacaaa gcgatcaata aaattgcctg taccagcaca tagagttcac cgcgcgattg
     2341 tttggagctc atgggtttgg gtatacagtt cccgtaatac ttggaatatc tccccatagg
     2401 ccttcggagg cttgttcgat tctttctctt tgcgtgcatt gcggatcaag gttctcagat
     2461 tttgcacatc caagcttgga tgctgagcaa tcaattcggt aagcacttca tcattactaa
     2521 gtaagcgttc tcgcaaacgt tccatgcggt gtaaatgagc ggtctctacc cttccaggtc
     2581 cttcaataat ctcaatctgt ttttttatac cctggatctc atcatccgtt agcaaacgca
     2641 tgagttttcc taagtactgc ttatgacgac gaatcccacc aaaggtcttt atcttggcag
     2701 tctccaagag agcgcttcgc aaaccatctt cgattggaac tgttttaagt gattctgagc
     2761 taaagccagc cagggtctcg gctaactttt ggcgctcgct catttggcgc ttgagctctg
     2821 acttacttgg tcccgcatct tcatcgggat cgatttgatc aactttactc atgtgctcca
     2881 ttacgaatta atatagagcg gaaattaata acaaattact gtaaaacttc ttgcgcagcc
     2941 aatacaccac tacccttgag cttgatgcca aagcggttca tacccatctc acaaccgcta
     3001 atggctgcta gcagcgatag ctcattgcaa tcacccaaat gaccaatccg aaatagctgt
     3061 ccttttgctt taccaagacc tgtaccgagg gataagttgt agcgctcgaa tatcacttta
     3121 cgtagagcat cagcatcaac accctgtgga gttctcaccc cagtgagtac tggggaataa
     3181 acggcaggat ctttgcactg cacttctagg ccccatgcct caacagcagc gcgacatgct
     3241 tttgctaaat gctgatgacg cgcaaataca ttatctaaac cttctgccaa aatcatatca
     3301 atcgcttcat gcaaaccata nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nggtggcagc attaagcctt tttgagaacc gctcactgta
     3421 acgtcaacgc cccactcatc gtggcgatag tcagcagacg ccaatcctga gatcgtatct
     3481 accatcaata aggctggatg ctttgctgca tcgatagctt tacgaactgc agcgatattg
     3541 gaagtgactc cggtcgaggt ctcattatga acaacacaga ccgctttaat ttgatgattg
     3601 gtatctgcct tgagacgctc ttcgatcttg gccgcatcga ctccatgacg ccatccttct
     3661 gcaccaggca taccaacaaa ctctgttttg attcctaaac ggatcgccag cttattccac
     3721 aaagtagcaa agtgaccggt ctcatacatc aagaccaaat caccagccga taaggtgttc
     3781 actaaagcac cttcccatgc tcccgttcct gaacttggat aaataatgac cggatgcttg
     3841 gtcttaaaaa tagtttgcat acccgatagc aatttaaggc ctagctcacc aaactcaggg
     3901 ccgcgatgat cgatagtttg atagctaatg gctcgtagca ctctagccgg cacagggctt
     3961 gggccgggaa tatgtaagaa atgtctacct gagggatgtt ggtccagttt tagcatgggg
     4021 ggtcaaattg ataagggatt gatctaaaaa tacaaagtcc tatttatacc agttcccaat
     4081 gcgactcgcc ttgacctagg ttaggagttt attgggctta gcctacccgc aaggcattct
     4141 gggtaatgag ttttgtgttt tgtaaactga attagactgc tactgttgac ccctcattaa
     4201 aaggactttc atgaattatg ttgcggtaat tggcacgggc acaatggctg caggaattgc
     4261 tgccggcttt atcgaagcag gtctcgccgt cacgattttg gggcgatctg cggataaggc
     4321 caaagcctgt ttagatgttg ccatcggctt agtacaagcc gaagggaatg ctaagcaccg
     4381 ttgtggactg attgatcaat ggaatgactg ggacgattgc atctgggtgg tagaaaccat
     4441 tgccgaggat cttgcattaa agcaagcact ctttcggtca ctcgatgagc gtgtcccagc
     4501 acatattcca attgggagca atagctctgc ctatccaatt agcaagatag ctgaaggcct
     4561 aaagacggca gagcgcatga tgggcgctca ctactttatg cctgcagaag ttgtgccctt
     4621 ggtagagatt gtgatgggtg aaaaaacaga actcgcgcac gctcaacatg tctgtgagct
     4681 ctataagtcg gtcaataaga aaccggttct tgttaagaaa gatattcctg gatttttagc
     4741 caatcggata cagcatgcat tgatgcgcga agccttacat ctaatcgatg caggaattgc
     4801 gtcacccgaa gatgttgatg acgcggtgcg ctacagcttt ggctttcgct acgccgccgt
     4861 tggccccatt actcagaagg agttatccgg ttgggacgtc agctgtcatt cggcgaagga
     4921 gatttacccc accctatgta acagtaatgc aatacctcaa tccatcttgg atatggt
//
LOCUS       DDPQ01000073            6892 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_24348,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000073 DDPQ01000000
VERSION     DDPQ01000073.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_24348"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    189..210
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6671..6692
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1574 a         1758 c         1659 g         1857 t
ORIGIN      
        1 cagatgaaca agatattggt ggtgtccact tgcaagaaat cttgattagg gtgtttgcgt
       61 cccccttgcg gaggcactga agccatcgtg ccctcaacaa gtttgagtaa tgcctgttga
      121 acgccctcac ccgatacgtc acgagtgatc gatgggttat ccgatttacg ggagatctta
      181 tcaatctcnn nnnnnnnnnn nnnnnnnnnn tgggctttct caatgttgta gtcgcagttt
      241 tgtaaaagct tctggatgat gttctccaca tcctcgccca catacccagc ctcggttaag
      301 gtggtggcat cggccatcac aaagggcaca tccaacatgc gcgctaaggt ctgagctaat
      361 aaggtcttgc ctgaacccgt tgggccaatt aataagatat tgctcttggc taattcaacg
      421 ccatcgacga gtggtttgtt gttacccgct tttgtatttg gacccgattt agcattctta
      481 ctggcatcct gtttatcttc ctcgacctgt ttcactttag gcgccggcag atgcatgaga
      541 cgtttgtaat ggttatagac cgccactgct aaggtcttct tggcaaggtc ttgtccgatc
      601 acatattgat cgagattaag acgaatatct tgaggggatg gcagtgcatc acccttactt
      661 gctaagggtt ctttagcaat ctcttcctga ataatgtcgg tgcaaaggtc aatgcactca
      721 tcgcagatga aaactgatgg tccagcaatg agttttttga cctcatgctg gcttttgcca
      781 cagaaggaac aatagagtgt tttatcgctt gataggattt cgctcattgt gtcctttctt
      841 agagcgctta actaccgcgc ttctcaatca ccttatcaac caagccatag tctttggctt
      901 gctcggctga cataaagtta tcacgatcgg tgtctttggc gatggtctca atggtttggc
      961 cagttcgatc tgacaaaatt ttgttgagac gttcgcgcaa gtacaagatc tcccgcgctt
     1021 gtatctcgat atccgatgct tgaccacgag ctccgcctaa gggttgatga atcatgactc
     1081 gagcattggg taatgcatga cgcttgccct tctcacccgc agcgagtaag aacgcgccca
     1141 tactggcggc catacccatg cacaaggtgc tgacatgggg ctttataaat tgcatggtgt
     1201 cgtagatcgc aagcccagca gatactgaac caccggggga attaatgtag agcgagatct
     1261 ctttatcggg attctcactc tccaagaaga gtaattgagc gatcactaaa ttagctgttt
     1321 ggtcgttgac ctcgcccacc aaaaagacca cacgctcttt taataagcgg gagtaaatat
     1381 cgtaggcgcg ctcaccccgt cctgaggtct cgaccaccat agggaccaaa cccagggctt
     1441 gggtatctag tgcgctcatt ggctcgtata tcggactcat gtgctctgct aactttctga
     1501 ttggtgcatc aattattagt aatgggtcta gattaactca ttttgctgag atcttcgaaa
     1561 gtgaccgctt tatcggtgac cttcgccaag ctacaaatat gcttcaccac gttctcttcc
     1621 aagacaacgg cctcaacctc tttcaggcgg ctaggatcgc tgtaatacca acgcatgacc
     1681 tctttagggt cctcataggt cgctgcggtc tcttcaattg aggccttaat ttgctcgggg
     1741 gttgcggtca gattttgttg ctttacaaga tccgagagga tcaagcccaa acggactcgc
     1801 ttattagctt gctcggcaaa catctcggga ggaatggggg catctttggc attggggatg
     1861 ccacgctgaa tgagatcatt acgcgccgcc tccatcaaac gctcttgctc accagtaatt
     1921 agcgacttag gaacatccaa ggggcattgc ttgaccaact catccatcac ttggttttta
     1981 aggagcgctt cggtgcgacg ttttacttcg cgatccatat tggtcttgat ctcgctacgc
     2041 atcttctcga cgccgccctc agtaataccg agcgacttcg caaactcttc gtccacaggc
     2101 ggcatatgag gccaagcgac atccttcatg gtcacggtga actcggcggt cttgccagcc
     2161 acttcagcac catggtaatc atccgggaac ttcattggga aggttttgct ttctcccttt
     2221 ttaaggccca atgcggcaga ctcaaactca ggcagcattt gtcctgcgcc aattacaaac
     2281 tggaaatcgt ctgctttgcc gccagcaaac tcaacgccat caattttgcc aacaaagtcg
     2341 atagtgactt gatcatcttt ttgagcgctc gtatcactgc caccatcacc gtggtcgcca
     2401 gcctcaccac gagagtggta atgcacgcgt tgcttacgta agacatctag ggcacgatca
     2461 atctcagggt cattcacttc tgttgtgtaa cgcgtgagct ccgaggcgct gaagtcaccg
     2521 atcttgacct cggggagcac ttcaaagaat gcatcaaaca caatctcatc ggcatcgatc
     2581 tcactcttgg gctcaaggcg aggttgactg gctaacttaa gaccttcttg tttgctgagc
     2641 tcataaaaga gttgtgtggc tttgtcgtat tgcacttcaa aatccacttg cattccatat
     2701 tgctgctcca ccattttctt ggggaccttg cctggacgaa atcccggcat cttcatggat
     2761 ttactaattt tggttaaacg ggcttcacgt gcctgattaa tatctgcacg attaaattgc
     2821 agagtcattt tgcggtctaa gctacctaag ttttcgattt gcactacagc ctctttagtc
     2881 aaggttattt gtgaacacta agccgagcct tgtggtgcga ggagggggac tcgaaccccc
     2941 acaccatttc tggcgtcagg acctaaacct ggtgcgtcta ccaatttcgc catcctcgct
     3001 atatctcaag cgctgcagac actacccagc caagaattca cataaaaggt agattctaca
     3061 atgccttaca gagtagagct acggcgtgca cggcaattcc ctcacctcgg cctagatgac
     3121 ctagggattc gttggttttg gctttgaggt tgatatggct tggggtgaca tgtaggtcac
     3181 tcgcaatatg acgaatcatc tccggtaaat gactggcgag cttcggcttt tggcagatca
     3241 cagtagcgtc cacattaccc acctggtagc ccgcagcctg aaccttttgt aaggcggcgc
     3301 gtagcaacac cctgctgtcc atatccttaa agcgctcatc atcgtcgggg aacatttgtc
     3361 caatatcgtt tagtcccgcg gcacctaata atgcatcggt gatggcatgt aaaagcgcat
     3421 cggcatccga gtggcctaac aaacctttct catagggaat atgcacccct cccagtatta
     3481 atcgacgccc tggcactaag gcatgaatgt cgtaaccttg gccgatccga aacggaatac
     3541 tgtgcgctgt ttgtgtcatg ggtctattct agtgtggtgt tcgcagaacc agctccatca
     3601 actcccaatc ggctggatgc gttaccttga aattgcgtgt tgcaccctct accaagagtg
     3661 gttgtccgcc ggctctttca atcgcactag cctcatcggt gatgttggct cctgctgcaa
     3721 tggcttcatg caaggcatcg tgtaatacct tgagcgggaa catctggggt gtttgcgcct
     3781 gccataagcg tgaacgatca atcgtaccct tggcacgcat cgcgtcctcg gtggtctctt
     3841 tcatggtatc ggctacaggg attgctaata agccgccatc aagatcccgt ttggaatccg
     3901 cctgggcttt gaccagcgta atcagtcgct caatcaaggc cggtgtaata ccaggacgtg
     3961 cggcatcatg aaccaagatc cagtcctgtg catgcactcc ggctttttgc attgcggcca
     4021 aggtatttaa tacggtctgt tggcgtgtag ggccaccggt ctgcaagaac tcaaagcgct
     4081 tcacgggttt tgggtagcat gctttaaggt caacgctcag ttgatcagag ccaggactca
     4141 tcccaatcca gaccgaatcg atctcagggc actcaaagaa ggcatctaag acatagagca
     4201 gcatgggctt tcctgccaga tgcctaaatt gcttaggctg gtctccgccc aagcgagccc
     4261 ccattccagc agagggtaat aaggcatgac agcgtctttg gggattgctc atgtgcgttt
     4321 catcttcaaa tacatacctg attctataat ggtcctagat gtcggatgct gtcaatccag
     4381 cggcagcgcc aaggcctgct gctttactaa caccccctat cccggtgctt cgggctgggc
     4441 agcgctttac cttcaaacag ctcgtaggat cctctgatgc agccctgatt gctcaaaccg
     4501 cctcagcgca tcgcaatcat tacagccaaa tggtggttct ctgtgcgagt gcactcgatg
     4561 cccagcggct tttggaagag atccccactt ttgctccgca acttcgtatt cgcttactac
     4621 ccgattggga gatcctgcct tacgatcact tctcacccca tcaagattta atttcagaac
     4681 gcctagctac cctctatgag atgcaaaacg ggagttgcga tctcatattg ttacccgtga
     4741 ccacggcctt acaacgctta gcaccccctg cttttttatc tgcccacaca tttttcttta
     4801 aacagggtga acacctcaat gaagaggcat tacgtttgca attacagcaa gcaggatacg
     4861 acccagtgag cgcggtcatg cgccccggtg aatacagtat ccgtggtggc ttgattgatt
     4921 tatttccgat gggatcgtct ttgccctatc gcttggattt atttggtgat gaaatcgagc
     4981 agatccgtgc ctttgacccc gatacacaac gcagtcttta tccggtcaaa gagattcgtt
     5041 tgctgcctgg tcatgagttt cccttcgatg aaggatcgcg aaccgcattt cggggacgct
     5101 ggcgtgagta ttttgagggt gatccgactc gttgctcgat ttataaagat gtgagctcgg
     5161 gcattccgac cgcaggtatt gagtcttatt tgcccctctt ctttgatgag accgccacaa
     5221 tctttgacta cttcatgcgt agcgagagcg ctgccagtga ttcagtatgt ttggtctatg
     5281 tgggtgatat tgagtctagc attacacagt tttggaaaga tacggagcaa cgctattcct
     5341 ttctgaagca cgaccccgag cgtccggtca tgaatcccag cgatttattt ctggggctag
     5401 atcagttttt tatccatgcc aagagccatc cgcgcattgt cttacaagta ggtctcgagg
     5461 aaggtgccaa gaaagatctt gatgagagcc tcatcttctc ttctctacct gacgtctctg
     5521 ttcatcgtcg tgatgcagac cccctcaagc taattcggca gttgctatcg caaaatattt
     5581 ggcgtgtgat tatttgtgcc gaaagcgctg gtcgcagtga gtccattcgg cagctcatgg
     5641 aagaaagtaa tttaggcgag agttctgata gtgcctccct ctttccattg catccttcat
     5701 ccgctgatag tgttgcagag tttttaaaag gatcggagcg tttttctctt tgtatctcac
     5761 cactctttaa tggttttact tggcatgccc atggcgttct catcattaca gaatccgagc
     5821 tctttaccca aaccgcacgg cagcgtcggg gcaaagaaac cagtaacacc gaccccgata
     5881 tgctctttaa agatctgtcg gaacttaagg tcggcgatcc ggtagttcat gtagaacatg
     5941 gcattggtcg ttaccaaggg ttggtcttac tcaatatggc tacttctaaa gaggctccgg
     6001 cctttgagga gttcttacat ctcgtctatg cccaaggtgc aaccttgtat gtgcccgtgc
     6061 agcaactgca tcaagttact cgctatgcag gcgctgatcc agactcagca ccgctacatc
     6121 aactgggttc ggggcaatgg gataaggcaa agcgcaaggc tgcccaacaa atacgtgata
     6181 ccgctgctga gttacttaat ctctatgcag cgcgcgcggt tcgcaaaggt catgcctttg
     6241 agttctcggc gcatgattac gccgcctttg cagagagctt tgggtttgaa gaaacgccag
     6301 accaagccaa tgcgattgct gccgtcattg gtgatatgac cagtggcaat cccatggatc
     6361 gattggtgtg cggtgatgtc ggttttggga agaccgaggt agctctcaga gccagctttg
     6421 tagcagtgat gggtgggaag caagtagcca tcttagcgcc taccacccta ctcgccgagc
     6481 aacacgctgc cacttggaaa gaccgctttg cggcttggcc cgtgcggatt gtggaactct
     6541 cacgctttaa gaccaccaaa gagattaatg cagcactggc ttcaatcgcc tctggagagg
     6601 ctgatattgt gattggtaca cataagctct tatcgaaaga aacaaagttt gagcgcttgg
     6661 gtttggtaat nnnnnnnnnn nnnnnnnnnn nncatcgctt tggggtgcgt cagaaagatg
     6721 ccctcaaggc catgcgtgct gaggtcgata tcttgaccct aaccgctaca ccaattccga
     6781 gaacattggg gatggcactc gagggcttgc gtgagttctc ggtgattgcg actgcgcctc
     6841 agaaacgatt agcgattaag acctttgtac ggcgtgaagg cgatggcgtg at
//
LOCUS       DDPQ01000074            5278 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_23371,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000074 DDPQ01000000
VERSION     DDPQ01000074.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_23371"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1947..2144
                     /estimated_length=198
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1316 a         1125 c         1243 g         1396 t
ORIGIN      
        1 atgcggtgat ctgtgatgcg gccctgtgga aagttatagg tccgaatgcg atcactgcga
       61 tcaccggtgc ctactaaaga cttgcgggtt tgggcctgct ctttttgttg ggcattgcgt
      121 tgtgcatcca tgatgcgcga gaccaagacc ttcatggcct gatctttatt gcgatgctga
      181 ctgcgatcat cctgacattc cactacgatt ccggttggta ggtgggtaat acgcactgca
      241 gagtctgttt tgttgatgtg ctgaccgcca gctccggatg cacgaaaggt atcaatgcga
      301 agatctgccg gattaatctt gatggcctca atttcatcaa cttctggcat cacagcaacg
      361 gtacaggctg atgtgtggat tctgccttga gtctcggttt gcggaacccg ttgcacccga
      421 tgaccgcccg actcaaactt cattttggca tatacatcag tgccacttag tcgcacaatc
      481 acttctttat agccacccag atcagattcg gcctgactca caatttcagc tttccagccc
      541 tgacgctctg catagcgggt atacattcgc aatagatccg cagcaaagag ggcgctttca
      601 tcgccgccag tgccagcacg tatctccaag aagatattac gaccatcgtc ttcatcttta
      661 ggtaatagaa gagtttgtaa gcttttctcg agatctgtca tacgaccttc agcttgtttt
      721 tgctcctcgt cggcaaactc tttcatttcg ggatctaggc gcatgtcaga ggcagctttg
      781 gcatcttcct cagccttttt ataaagacca aattgctcga cgatggcaga aatatcagca
      841 tgttcgcgcg tgagcttgcg atagacgtcc atatccttgg tggagtcttc ttgcatcaaa
      901 atagtattga gttcagccaa acgggcatcg agatgctcca gcttggcacg catgctggat
      961 ttcatctaat gactatctga ggagctattt ttatctaggc tggaatgagt ggtaaataat
     1021 ttaggaagca acttaatcag agcatcgcgc tcagaaccgc tggcatgttg gagagcatgt
     1081 aaggagccat gtaaaaactt attagttaat ccttgagcca ttgcattaag gacttcttgg
     1141 ggatcatcac cgcgtatcaa acgcttgaga gcgcgctcta gctcaatttg gcgtaagcgc
     1201 tcaccttgct gctgaatatc ttgtattagt gggactgcat tgcgtccttg gagccaatgc
     1261 ataaagttac caacgcgctc ttcaataatg atctctgcct gactaacagc agcttggcgc
     1321 agattagcac cggtctgtgc catcgttccg aggtcatcca cggtatagag gtaaacgtca
     1381 ttgagccttg aaatttctgg ttcaaaatca cgaggcacgg ctaaatcaat catcaccatg
     1441 ggtttgcgac gacgcagctt taaagcgctc tccaccattc caagaccaat gatcggtaag
     1501 gggctcgcag tcgatgacac aatgatgtca aactcgtgca agcgtgtggg tagatcattg
     1561 aggcggaatg actcagcctc aatattttgg gccgatatag attcagcgag ttcttgaccg
     1621 cgttcaatag tacgattagc aatcgcagta gcttttggtt tacgggctac aaaatgggta
     1681 gcgcataagg taatcatctc accggcgccg atgaacaaaa tcttttgttc gccaatggat
     1741 tcaaaaatac gttcagccag tcgcactgag gctgcagcca tcgaaatcga atgtgcccca
     1801 atttcggttg agcctcgcac ctctttagca acggaaaatg ttttttggaa tagttgatta
     1861 agataggtgc ccaaagcgcc tgcatcattg gcagtacgca ccgcatcttt catttgacct
     1921 aagatttggg tctcaccaat caccatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccaaga tgggagcgaa
     2161 gatcgaataa ggcgtcctgc agaccttcgg ggtcaaaggc tactttctcc cgaacggcaa
     2221 ctggcgctgt gtgatggtta atgcccaaag tcaacagctt catatcgtga attatagatt
     2281 tcttgggctc aataggggtg ttttcaatgg ggtttttagc ttatttgttg gttggaggca
     2341 cagtgggctg ggctgcaggc cgctattttc ctgggttttc ccctagtaat aagggctcag
     2401 caagagcgaa acgtcctaaa tcgatctttc tttggaccat tggtcttggg gcgctgggag
     2461 ccctagcgac tagttatggc ggccaaatga ttggcctatt tacctcaggg caaatgctgg
     2521 agtgggggag tgcgattctg ggagctttta gtttgtccca aatttatctt ttacttagga
     2581 gaccttaaaa caaattctga ttcaactcca ccaacgaccc atttgatcgt attttgttga
     2641 tgagtagtca ggtgtttatt tgttttacta aagtgcccac aacttccttg atcccctggg
     2701 aaaataaatg gctgcattgt tttataaact cccaggcctg agggatggga ggcacgcagt
     2761 atgcaatgat tatttgcatt gcatatgtat tcctcaaacg actgtgcatg cgagccccag
     2821 agaagccaga ccagatgctt ttgagagctt gatagttttt tgatgatcgc cctaacaaaa
     2881 ctagcccagc ctaattgact gtgcgagccg gcctcaccta gtcgaacgga tagtgcggta
     2941 ttaagtagca ggacaccttg tttggcccaa tggcttaagt caccattttc taaggagcca
     3001 aatccctcga tttgcagagc tttattaatg ttacgaagag agcttggaaa ttgtttggag
     3061 cgaagggcaa tatctcgcgg aatagaaaat gcaagacctt gtgctaatcc tggcgaatga
     3121 tatgggtctt gaccaagcag aattactttg acctcagaaa caggagtcaa tttcagagca
     3181 ttaaacagga gctctttatc gggtcgaatg aattgccccc gcatttgata ttcttgatca
     3241 aaagcatcac agagctgttg ccaatccctt gaaacaaagt aatccgatag aaatacttgc
     3301 caatcgttcg gaatcaatga aagcaagttg ctcactgtta catcaaatca gaaagaattg
     3361 gatttgttcg ttttgatctc atactgaacg gggctattga gtttgagagc aatgctggcg
     3421 ctatattctt tatcttgccg aaagtaatga aagcttaatc gtttgctgtg tttaaggctt
     3481 gctagaacag ggtcgagtcg actggagttg acgcgttgac cattgatgct actaatgagg
     3541 tcgtgcggtg ccagcccagc ttgttgggca agacccccat cgagaacgtg ggttagctta
     3601 agccagccat gactgtcggc gtgacggatc cccagcgata gcttgagatc atctaggaag
     3661 cgagctgatt taagggagac tgaaattccg gcgatctggg gtaaccacag ttgtaaaggc
     3721 agatcttggg tgccatggat gtaattcttt ttaaagtctt gccagatttt attaaaggta
     3781 atgcccatga tctgactaat tgctaaatct agtgagtatt gattgattcc taaaaaaggt
     3841 ttgccatgct ctttccaaag atagcgcatg agatcatcca acgatatctt gccactacta
     3901 tgatggcgca tcacgagatc aagacctaga gcaatgagag cgccctttgc ataataacta
     3961 actaccgcat taggagtgtt ctcatccatt tggtaatact tggtccaagt atcaaaagag
     4021 ctatcagcta cagtttgttt gtgacgtcct ggattgcgta agacgccatt ccagttattc
     4081 gtaagcagct caagataacg cggtaggcta atcgttttgc ttctgagaag ttgaaggtca
     4141 tcatagtagc tggtaaagcc ttcaaagagc cacagtagac gagtatggtt gcgcccaccc
     4201 agatcatagg gttggaaagc cttgggttga atccgcttga ctaaccaggc atgaaaatat
     4261 tcatgactgc ataagcccaa gaactcctca taggttttct tgggggtctt ggcgtcatga
     4321 tcttggtaag gaagttgatc gcgtttgcac aaaagcgcag tgctattgcg atgctcgagg
     4381 cctccatagc catctaatgc agcattgaca ataaataagt agcgtgagaa cggtgccttt
     4441 ggtttgcggg gctcaaatag acgaatggtc tccgcacaga ttttggagag atctcgggca
     4501 agctgctctt tatctaaaag ctcagattgc tcagctgtaa gaccttggat cgccatggca
     4561 tggggggtgc catacaattg ccactcaatc agatcaaagt gacccatggc aacgggatga
     4621 tcaatcaggt catcataatt tttggcaaga taaaaaccaa agccttgcaa atctgtcttt
     4681 actggcgcaa gtgtgcaatg ggcattccac cctaacaggg actggtcggt tggtggggca
     4741 atcgctagag agcaaggtag atgagcttga ccttgaacgg ctagacatag actggtggga
     4801 ttaaaaaaac cgcgctcttg atctaagtaa gcggtacgta cggattgatc aaaagcatag
     4861 accgtagtga tgatttcaca gctagcgcta tttttgggta agcgccagcg atcgttatcg
     4921 agccgctcaa gtggaattga tttgcgcaac ccagttttct taatcgaggt aaatgcagaa
     4981 attgtttcaa tatgtttact aaagtctcgt attaaatagc tacccgggat ccatgccggc
     5041 atcgcaacta tttgtcctgc tggatctggg cgctcgatca ctagttttac gatgaaacga
     5101 tgcccgtata aatcatcggg ccagacggtg tactgcactg cgggcatggc attggcttgc
     5161 atggtcgtaa ctatttgagt gatgctaatt tcttctcaat atcgacaagt tgaactgcgc
     5221 ctgggaaccg actgccgtca gtaaagaaaa tagtgggcgt gccattaatg ccatagtt
//
LOCUS       DDPQ01000075           12354 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_9411,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000075 DDPQ01000000
VERSION     DDPQ01000075.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 12354)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12354)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12354
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_9411"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    6896..6961
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3222 a         3191 c         2598 g         3277 t
ORIGIN      
        1 tcgtcgtaaa taatcagcgc atcttcgccg cgatcacgga aatactcacc catcgtgcaa
       61 cccgtatatg cggataaata ctgcattgct gcagactctg atgcgcttgc agcaaccaca
      121 atggtgtatt ccatggcacc aagttcggtg agtttgcgaa cgacgttcac aatagtcgag
      181 gctttttgtc cgattgcaac ataaatacaa taaaggcctt tgcccttttg attaatgatg
      241 gcgtcgattg ctacagccgt tttaccggtt tgtctatctc caataattaa ctcgcgctga
      301 ccccggccaa ttggcaccat tgagtcaatc gccttgagtc ctgtttgaac cggcgtacta
      361 actgactgac gcgcgataac tccgggcgca actttttcaa tgtaatcggt aagttttgta
      421 ttaattgggc ccttaccatc aattggctgt ccaagtgcgt ttactacgcg tcccaataac
      481 tcgggtccga ttgggacttc taaaatacgc cctgtgcatt taaccgaatc accttcctta
      541 atatgcgtgt attcgcccaa aacaacggcg cccacagagt ctcgctctaa attaagtgct
      601 aggccaattg tgttgttcgg aaactccaac atctcgcctt gcataacgcc agagagtcca
      661 tagattcgac aaataccatc ggtcacagaa ataaccgtgc cttgattacg aatctttgaa
      721 tcaaccccta attcattaat tcggcttttg atcagttcgc tgatctcgga agggttcagt
      781 tgcatcaatt actcctagat ataaatcgta tgttcttaat taatcgttac gcgcttaaat
      841 tgatttgcat ttgtactaaa cgggttttaa tcgagctgtc caaaacctcg tcgcctactt
      901 gtacgcgaac accgccaatc agctctgggt caaccacaac cgtcgggcgc agctcttttc
      961 caccaaatcg tttttttaaa cttgctaata attcagttaa agccccgccc tccatcggaa
     1021 atgcggatgt aatgattgcc tctgctgctc cctcgctctg atttttcata gcaataaatt
     1081 gctttgcaat ttcaggtaat gccactaagc ggtggttatt ggctaacaaa tttaagaagc
     1141 tggcgaggcc ttgatctggc ttcgacttaa gaaggccttc aattaatttg aataggtcat
     1201 ttgagcttaa ttttgggtta ttggccaacg caatcaattc agcattctca aacaacgccg
     1261 ccaagtcttc taattgattt gcccaagcac tcagatcagc aggcttagcg cttgcaaata
     1321 aagcctcagc ataaggccga gcaattgtgg caatgtcggc catttatagc tctgccttta
     1381 attggtccaa taacgcggca tgagctttgg gatcaacctc gcggcgcaag atttgctctg
     1441 cgcctttgac tgctaggcct gcaacttgat tgcgcaactc atctcgcgcc ttaccaacct
     1501 gttgctctgc atcggctttt gcttgggcaa taatgcgtgc ggcttcggcc tgagcatttg
     1561 ccttaatttc ctcagcagcc atttgcgctc ttttttcagc ctctgctaca cgctgaacac
     1621 cctcttgccg cgcagacgcc attgccaatt ctgctttttt ggttgcattc tcgagatcca
     1681 tcttgccgcg ctcggcggct gccaagccat cggcaacttt agcagcgcgc tcatctagcg
     1741 ctttcaccaa agggggccat acaaaacgcg caacgaccca ccataagatg aagaaaacga
     1801 tcatttgcgc gaataaggtc gcgtttagat tcacgatgtt cctttcaaaa catttcgctg
     1861 ttgtgggcca ctaatgtggc ccgaaacaaa attacttaat aacggcaaga agtgggttcg
     1921 caaaagcgaa caacattgca acaccaacgc cgatcaagaa cgcagcatca atcaatccgg
     1981 ccaacaagaa catcttggtt tgtaattcgt tcatcaattc tggctgacgt gcagcaccct
     2041 caatgaattt tccacccatt aggccgattc ccaagcaggc gccaagggct ccgaggccaa
     2101 taatgagacc aataccaatt gcggtgaggc cttgaatgtt tgctaaaaat gcttgcatgt
     2161 tgactcctaa gtaataaagg tggtgttaaa aataaaaacg gctagttaat gatggctatg
     2221 tgcctgacca atgtagacca aggtcaacat cataaaaata aatgcttgca acaaaataac
     2281 aagaatgtga aatatcgccc atgccgaccc tgcgatcaca tgtccaacta gtccgagcac
     2341 gcttagatca agactaaagg tccaaatgct tcccagcagg gcaatcaaca agaaaattaa
     2401 ttctcccgca tacatgttgc caaatagtcg cattcctaat gaaacgccct ttgcaacata
     2461 ttcaataata ttcaacacta aattaaacgg cgccaagtac catttcgcac caaatggggc
     2521 ggataaaagt tcatgggcaa atccgccgac gccttttgcc ttgaagctat agaaaaacac
     2581 taagagcaga actgcaaatg acattcctag tgttgcattt aagtcggtcg tcggaactat
     2641 tttgtggtga ggaacatgta agccaaaatt ttctaagaaa tgatttaccc ccacaaccca
     2701 atcaaccgga acgaggtcga gcgtgtttaa caaaataatc caacaaaata caaatagggc
     2761 tagtggggca atatagctac gatcaccgtg cacaatactt tttgattgcg tatcaaccat
     2821 ctctaccaac atctctacta gggcctgaaa gcgccctggt acaccagacg tcgctcgtct
     2881 tgcggcaacc aacaaaaagc caacggcgat aagacccatt aagagggtcc aaaaaagggt
     2941 atctagatta ataacgctga aatcaataat ggaggtctgc gcaccaccaa tattgttaag
     3001 gttttgcaaa tgctctgcaa tgtaggctgt gggtgttaaa ggctcgctag gagctccgtg
     3061 tgctgcactt gccattttta agcaattccc ttgttacaca aaattaacac tgctcaccgc
     3121 caaaaccacg caagccaata acacttgagc gtgacaaagt aagtgaccag caacggtatc
     3181 cactgcagat ctgggtaact aaacgctacc cacacaaaca ttgcaatcgt tacggcaatt
     3241 tttataaatt cgccagaaac aatcgccgct aacaaactcc caggattctg gtttttcatt
     3301 cgcttggcgg cgctgagtcg taaagcaaac agcgatgccg gaaaaaaccc gataaatcca
     3361 cccaaaagag ctgagtaagt ataaaggctc agaccttttg gaattccaac aatcgaccaa
     3421 gcgagcgcac tcaataaggt cacgaccaac tgaaggccga taaccgccca gggagaaagg
     3481 gtaaaaaatg ctctgctttt attctgccgc agggcatcca tctctttctt gctataggtc
     3541 cgataagcca actgctgctc atcgacctcg tcccaagcgt gcgctgaatg atcttttttg
     3601 ggaatcaatt tagcaacaac attctattat ttgccccgat caaggtcaat tgttttaggt
     3661 acttttatta cttaatttga ttcaataccc agttttttca taagggaatc tagctcatca
     3721 aattgactga agtaaaaccg cagctctcct cccttgccct tatttttaaa ctcgacctct
     3781 aagcccacaa tatcggccat attctgcgcc aatcgtttta ggtcttgatc ggcctggctc
     3841 gacttccccg cagagccttt taatggtttt ttctttgcca agtctcctga aattaaggtg
     3901 gccgaagctg ccaatcgctc tgtctcccga actgaaaggc cctgggccac aattttttga
     3961 gccagcgcaa cctgactaga cccaggcaaa gaaagtaggg cccttgcatg ccccatatcc
     4021 agctcgccag ctattagcat tgcttgcacg gcttttgcca gctgggatag gcgcaacaaa
     4081 ttactaattg cacttctcga ctttccaacc gacttggcgg cctgttcatg agtaaagcca
     4141 aactcctcaa taagacgtgc aaggccccgt gattcctcaa ggggattgag atcttcgcgc
     4201 tgcatgttct caatcaatgc catagccgct gccgcctgat cgtccgcgtc cacaatgcgc
     4261 accggcacct ctttaagccc agcaatgctg gcagccctaa agcgtcgctc gcccgcaata
     4321 atctcgtatc gacctgttcc taaccctcgt actaataaag gctgtatcac accccgctca
     4381 cgaatactgt cggccaactc ctttaaagga gtctcgtcca ttatttgtct tggctgatat
     4441 ttgcctgcct gcaacaaatt aagcggcaag gcgctagggt tgtcgccacc aaattcactc
     4501 gccatatttt tattggaggc cccaagcaag gcatccaagc ctcttcccag tccttttttc
     4561 ttatttacac tcatttttta cctacattgt tttaatgcgc tcaaccattt ctccgccgaa
     4621 ttcaatatag gcttgtgctc cacgcgctga tttatcaaaa ataaccccag gcaaaccgaa
     4681 tgatggcgcc tctgctaaac gaacatttct aggaataatt gtcttaaaca ccttgttccc
     4741 aaaatgggtc ataagctgct ctgatacctg ttgttgcaat gtaacgcgag catcaaacat
     4801 gacccgcaat aatccaatta tttttaaatt gggatttaaa tttgcgtgca cctgtttaat
     4861 cgtattcacc aaatccgata acccttcaag ggcaaaatac tcgcattgca ttggcaccac
     4921 aacgccatcg gcagcacaca ggccattaag cgttagtaaa gataatgctg gcggacagtc
     4981 gatcaagata aagtcaaatt gatcctctat ctccgctagg gctagcttca atcgcatctc
     5041 acgattttct aaatcaacca gctctacttc tgctccggcc aaatcacggt ttgcaggcat
     5101 cacgctgtag ccagcagtgg gcgcgtacag aacggtctgt gccgcggtgg ccatgccgat
     5161 taggacttga taaacgctgt tttctaaatt tgctttgtca actccagacc ccattgttgc
     5221 gttgccttgg gggtcaaggt ctatcaaaag aactctttgt ttttgggcca cgaggcccgc
     5281 agccaaatta acggcggttg ttgttttacc aacgccgccc ttttggtttg caatacaaaa
     5341 tgttttggcc attttattat ttttcttttt tacttaattt gaaatactaa taatcgacgg
     5401 tctacttttt gcccaggcaa tgccagcgca cactcctcta aaagcgtcca ctctgcttta
     5461 gaaacctctg caatttcttc ttcgctgcgc tggctcttca ttgcaaacac caagcccgaa
     5521 ggttttagta ggggccttgc caactccaat agctttttta atcgagtaaa tgcgcgcgca
     5581 gtaactgcat cgaactcccc cgccatctta tgtgcaacct gctcaacgcg cattgccatt
     5641 acgctcatat tctttaattg caagccgcca cgcacatgct gtaagaaagc tgccttcttt
     5701 ttgcttgcct cgatcaaata aaggcaccat tgggtttgca aaatagcaag cgggatcccc
     5761 gggaggccgc ctccggagcc catgtcagca agctgaagtt tctgtgtttt gtctctcgca
     5821 gcacttccca aatagcgcat tagtattgga aaaattacaa tggaatcaat aagatgtaaa
     5881 tttatagcag aatccgtatc ctcaattgct gttaaattgt gcacccgatt ccatcgcaat
     5941 agctcattta aaaaataatc catcgcttta atttgctcaa tattcaagcc cagatcggca
     6001 aggctgccct ctaaagtcag gccattcata gcagtacctg tgttttacca atgcctttct
     6061 ttagatgaac cagcagtaat gagatggctg ccggcgtaag cccagaaatt ctggaggcct
     6121 gccccaaagt ctcaggctga tgcatattga gcttttgcca aacctctaca gacaggcctt
     6181 tcactaagga atagtctaaa tctgggggca agcgaaggct ttcatagtgc tcatgtcgct
     6241 ctatctcagt tttttgtcgt tcaatataac cctgatactt gatcgatatc tcaacttgat
     6301 caacgatctg tttgtgcaat tgcaagtctg atattgctgg ggcgccctca gcaagctgag
     6361 ggtcggcctg cataagatcc cgataagatg cttcgggtcg tcgcaagatc tctgccaggg
     6421 tgcattcgtg cgacaaggcc tggcctagga aggctgccag tgctggggct gctttatggc
     6481 gggggccgac ccgaaatgtg gcgagacgag atgtttcacg tgaaacatgc tcctgctttt
     6541 tctgaaaaaa ctcccattgc cggtctgata acaggcccat tgagcgccct ctctctgaaa
     6601 gacgggcatc ggcgttgtcc tctctaagac tcagtcgata ctctgctcgg ctagtaaaca
     6661 ttcggtaagg ctcttgcacc ccacgggtaa ttaggtcgtc tactaaaacc cctaagtatg
     6721 actcgcttcg cttcgggacc catggatcgc gttctttaac tgcaagtgcc gcattaattc
     6781 cggccaagag gccttgggca gcggcctcct catatcccgt ggtgccatta atctggcccg
     6841 caaaatagag tccagacaac gccctagtct ccaggctatg tttaaggcgc cttggnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 natactctgc accaaatcct gctgaacatc gaatggcaag ctagtcgaaa taccatttgg
     7021 ataaaactca ttggtcgtca agccttcggg ctctaaaaaa atctggtggc tctctttggc
     7081 tgcaaaccgg tgaattttat cctctataga agggcaataa cgagggccca ccccttcaat
     7141 aacccccgta tacatcggcg agcggtcaag gcctccgcga atgatgtcat gcgtttttgc
     7201 attggtatgg gttatccaac aggccacctg ccgagggtgc tgctcaggcc gccccataaa
     7261 tgaaaaacat gggacaggat ctaaatcgcc gggctgctca gtcattaccg aaaaatcaat
     7321 tgttctgcca tcaatgcgcg gcggggtccc ggttttaagt cgcccctggg ggagcgcaag
     7381 ctcttttaga cgagctgaaa gtgtcattgc cgcaggatct ccagcacgcc cgccactaaa
     7441 gttcatcagg ccaatatgaa tctttccatc cagaaaggtg cccacagtta atacaactgt
     7501 ttgcgcccta aactcaagac cgttttgagt tttaaccccc aaaacccgat cgcccatcac
     7561 aatcaagtca tccgcagcgg cttgaaataa gctcaagtta gcctgatttt caaggcgcct
     7621 acgtatggca gccttatata agatgcggtc gccctgcgcc cgggttgctc gcactgccgg
     7681 gcccttgctg gagtttaaga tgcgaaactg aatgccagcc tcatcggttg cggccgccat
     7741 cgcgccgccc aaggcatcaa tttcttttac caaatgcccc ttcccaatgc caccgatgga
     7801 ggggttgcag ctcatggcgc caagggtttc gatgctgtgg gttaacaaaa gggtagcgca
     7861 acccattcga gcggacgcaa gcgcagcctc ggtgccagca tgaccgccgc ccaccacaat
     7921 gacatcaaat ttcttggaat aaagcatgga ttgccctttg tcgggcgtag atcatagcat
     7981 ttttactgtt tcacgtgaaa cacagtaacc caaaaagcct tttttaaatc ttaagtaatt
     8041 gatttaatta gaaaaaaaag ccgtgtaacc agatttggcg atgagggtca tcacaacaac
     8101 taaaaagatc tttcttacaa actggctgcc gtgtttaatg gctaatttgc ttccaatctg
     8161 actcccaata atattagcaa ccatcatgat caagcccagt ggccagtcga taaaacccag
     8221 gcttacgagc ataaacacgg cggcaaaatt ggtggtgaca ttcacgagct ttgttgcggc
     8281 agaggcgtga agaaagtcga aataaaaaag gcgcacgaag gcgaacaaaa aaaagcttcc
     8341 ggtcgcagga ccaaaaaatc catcatagaa accaataacc aagcccattg cgatggcctt
     8401 tatctcttcg tgcctcccca ccttttgggg tggtcgattc tccagcccaa agcttggcgc
     8461 aaaccaggta taggccaaca agataaccaa catgaagggc agggctttgc gcaagggctc
     8521 agaaggaaaa acgcttaccg caaaggcgcc taatagtgcg cctataaatc caccgataat
     8581 cgcggggccc aaaatattcc agggcagttg tgcgtgtttt aaataacggc gcgctgcatt
     8641 tatggttccg ccaattgaag acaccttatt ggcgcttaat aatgttgcag gatgtgcgtc
     8701 tgggtatgcc ataaaaagtg ccggcacttg gatcaatccg ccgccgccgg ctattgcatc
     8761 aaccaagccg gatgcaaatg cagtaaacaa aagtaacagc caaagataag atgaaaaatc
     8821 gaccagtgtt tccaaaaatt tcgctttgtt gttttatttg tttgtattgt tagcttctct
     8881 aaatatttaa gaagaattgc gagcgcccat aaattttgtg agcgcctctt tgcacgcctc
     8941 atccaaaata tcaatgggca ttcgttgtga cccctgaacg atcataagag cgtagggctt
     9001 ggcaagcgcc gcggcctctt ttgacaaaat caactcttcg ccctttgcgg gcgattgtga
     9061 gcgccaataa ttaatggcgg cctctaattc ttgaatagta ataaacagca tttaaacaag
     9121 gggggcttaa ccaatgtcca gcattgtgcc gcggcatttt ttactgccgc aataacaggc
     9181 gtaatccttt ttttctttct tcgttagctt gccatctaga tttaacgagt agtcataaaa
     9241 aagctcctcg ccaggaagaa gcttacgtct tgcaaaaata aacacccggc tttccccatc
     9301 caccgcaatc tcatcggcct cgcaattagg cttgcaagag tgatttatcc agcgcgcagc
     9361 attgccgcca acgtttgcgt cgatcaccct tccatcctcc aatgaaaaat aaaaggtgtg
     9421 gtttggttgt tctggatcgt gagggtgccg acgcaaagcc tccttccaac taatccgttc
     9481 accaatatat tcaatgattg ctgacccttt agggatttct ttttttacaa aaacgccgcg
     9541 gccatgaatt ttggaatttt taacggcaat aaatgacttc gccacgattt tcggggcttt
     9601 gttttttttc atgggattaa atgtctagat tgcgcgcaat caaagcgttc ttttcaataa
     9661 aggcacgccg aggctcaact tcgtcgccca ttagggttgt aaaaacctga tcggccatga
     9721 tggcgtcttc aatttgaacg cgcaacaacc ttctggtgct tgcatccatt gttgtttccc
     9781 aaagctgctg gggattcatt tcaccaagac ccttatagcg ctgacgacta atggcacgct
     9841 cagcctcgga aagaagccac tcaaatgccg cccggaaatc ttggactgcc tgctctttgt
     9901 ttgacttctc tggatcgccc cgacgaactc gactgcccgc caaaacccga ccggaaaggg
     9961 cggctgccgc cttcaccagg ctttggtaat cgtcacccgc cacaaagtct gaatttataa
    10021 ctgatagttt taggtttcca tgaattctgc gggcaagtaa gagccgataa cgatcggttc
    10081 gctcttcttt ttgaaccgtg atctcagggg ttacagctaa ggggtgctga ttttctgaaa
    10141 aagccttcct aaggcgctct gccgaggcgc ttgcagcact ctccgtgtca aaattaaggc
    10201 taacaccatc agccacagca cgcaaagcat cttcatccat tgtgcgcgaa agtcgatcaa
    10261 tgacggcctg tatcgtttga taattttttg ccaagtcttc aagtgcgggg ccggcaatct
    10321 tttcgcccga gggggtttca attgacgcct gctccagagc aatcttaaaa agcagctgat
    10381 ttaattcaac atcatcctta atgtattgct cactcttgcc atgctttact ttgtaaagcg
    10441 gcggttgcgc aatatagata tgcccgcgct caataagttc aggcatttgc ctataaaaga
    10501 atgtgagtag cagagtgcga atatgactac catcaacgtc cgcatcggtc atgataatga
    10561 tgcgatggta gcgaagcttg tcggccttgt actcctcaac cccaatgcca gtaccaagaa
    10621 cggtgatcag tgtgaccacc tcttggctgc tcaacatttt gtcaaaacgc gctttttcga
    10681 catttagaat ttttcccttt aagggcaaaa tcgcctgaaa tttacggtcc cgcccttgct
    10741 ttgcagagcc gccggcagag tctccctcaa caataaagag ttctgatttt gcggggtcct
    10801 tttcttggca gtctgccagc tttccgggca agccaatgcc gtcgagagcg cccttgcgac
    10861 gcgtcatctc acgcgccttt ctggcggcct cgcgcgctct agccgcatcc acaatcttgc
    10921 cgcataaaat cttggcgtct tgtggcttct ctgccaagta ttctgtcaag gcctgagcca
    10981 caacatcctc aacggggccg cgcacctcac tagaaaccaa cttatctttg gtttgacttg
    11041 aaaactttgg ctctggaacc ttaacagaca aaatacaggt caaaccttcg cgcatatcgt
    11101 cgcccgatat ttctactttt gccttttttg caacctcgtt ttcgtcaatg tacttattaa
    11161 taacccgagt cattgccgcg cgcaggcctg ttaagtgtgt gccgccgtcg cgctggggta
    11221 tgttgttggt aaaacacaaa acctgctcgc tatagccatc gttccattgc atggccacct
    11281 caaccgtaat cgttccgccc aaatcggacg ggcggtctgc aaccgcatga aaaatgttgg
    11341 ggtggagaac cgttttgttt tgattaatgt actcaacaaa gccctttacg ccaccagaaa
    11401 atgcaaaatc ttcctcttgg ccgctgcgct gatccactaa tcggatatga acgccgttat
    11461 ttaagaaaga aagctcacga atacgcttta ctagtatttc gtagtgatac tctatgtttc
    11521 caaagatttc ttcgtcggca agaaaatgaa cctctgtgcc acgcaggtcc gtatcgccag
    11581 ttactgatat tggtgaaacc tcaacgccat tctccttttg aatgttgcgc ttttgtacaa
    11641 cgccccgacc aaattccata tgatgagttt tgccatcacg ccgaatagta aggcgaagcc
    11701 atttggatag tgcgttgaca cagcttacgc ccacaccatg aagaccgcca gaaaccttat
    11761 atgagttttg atcgaactta cccccggcgt gcagttccgt cattacaatt tcagctgcgc
    11821 tacgcttggg ctcgtgcttg tcgtcgtact taataccagt aggaacaccg cggccattgt
    11881 caatcaccga tatagagttg tctgtatgaa ttacgacgga tatttcggta cagtgaccag
    11941 caagagcctc atcaatagag ttatctaaaa cttcaaaaac taagtggtgt aaccccgttc
    12001 cgtccgaggt gtcaccaata tacatacccg ggcgttttct taccgcctct aagccctcta
    12061 gaatttgaat tgaggatgcg ccgtattgtt ctactgtttt attttcttca gacattttta
    12121 tataacttac cttctcggct gtgcttatat gcgcattggc ataacaacat acttaaacga
    12181 ctctgaaccg ggaagagtaa taatggcgct actatttgcg tctcccagac taatttgaat
    12241 ttcattattt ttgatgttgg ctaaaacatc caataaataa gttacgttga acccaatttc
    12301 aactggctcg ccatcgtatt tggtttctat ttcttcttgc gcctcttctt gttc
//
LOCUS       DDPQ01000076            5533 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_10421,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000076 DDPQ01000000
VERSION     DDPQ01000076.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_10421"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2037..2070
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3541..3584
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1327 a         1262 c         1340 g         1524 t
ORIGIN      
        1 gccagactgc tccaccccgc gtctgagcct tagattctag catttttatg ccccaatgtc
       61 gagcaaagct ctagatagca ggcccttacg ggcgtatcat atggtctttg gtcacttctt
      121 tgcatctatc caatgacaat tagtaatcca gagtattcca attcatcgct tctaaagccc
      181 gttgatatac acggccccga tcaccacacc aaaaaaagtc tacccgcagt catgttggct
      241 gctattggcg tagtctttgg agacattggc actagccctt tatatgcatt aaaagaatgt
      301 tttgatccca agcatggcat tccattctcg gtagaggcgc tctatggagt catctccatg
      361 atgatttggg cactctttat tgtggtgacc tttaagtatg tcttctttgt gatgcgtgcc
      421 gacaataagg gtgagggtgg cgtactctcc ttaatggcgc tcgcactgcg ctccatcaag
      481 agcaacactc gacaatactt tttgattatg gtcttaggga tgttaggcgc ttgtatgctg
      541 cttggcgagt cggtcatcac cccggcaatt tcggtactct cagcggtcga gggaattgca
      601 attgccaccc cgcaattagc taatgcggtg attccaatat cagtcattat tttgattgct
      661 ttgtttgtga ttcaaaaann tttggtttat taccttggcc attttaggtc tcatgaatat
      721 tggtaaagcc ccagaaatta tttacgcatt taatccgctc tacgcaatta attttgtgat
      781 cgattacccc ttaacggcat acatcgtgat gggtgcggta gtgcttgtag tcaccggggt
      841 agaagctctt tatctggata tgggtcattt tgggcgcaat ccagtgcgct atgcatggct
      901 tcttatcgtc ttgccttgct tactgattaa ttacttgggc cagggtgcgg tcttaattgc
      961 gaaccccgct gcagccgcaa acccttttta tttaatggta ccggaatggg ctttgtggcc
     1021 ggtagttgct ttggctactg ccgcaaccgt aatcgcatcc caagcggtga tctcaggcgc
     1081 attctctctc gttaaccaag gcatcttgct cggtttcatg ccccgcatga atattttgca
     1141 cacctcggat tctgagcgtg gtcagattta tattcccgct gttaattggg cactcttaat
     1201 tatggtgatt gtgaccgtca tggaatttgg tgaatcggtc aacctagccg ccgcgtatgg
     1261 aatatcggtg agctccacca tgttgattac caccattcta ttaagtatcg tgatgcgccg
     1321 tgagtggcat atcaatccat tcattattgg gtttttgatc attagcttct ttgtgattga
     1381 tctcgcgttt tggactgcaa ccctcattaa gattaaagat ggtggttggt atccattagc
     1441 actcggactc ttgatattta cttgcatcat tacctggtat cgcggtaggc aattactcag
     1501 agataaattg attaaagagt caatcccgct ggagatgttt attaaaaatc ttctgcagca
     1561 tcctccccat cgggtagagg gtactgccgt gttcttaaca ccgcacattg attttgtgcc
     1621 agcagcgatg cttcataacc tcaagcacaa ccatgtgatg catcgacgga tcttcttttt
     1681 aaagctcagc acctgggatg ttccatttgt gcgtgacgat gagcgtttgt ccttaaacga
     1741 tttgggtggt aatgtgtatg tagtgcgcta tgtccatggt tttaaagaga cccctgatat
     1801 caataaagtg attgagctta ttagcaaaca atacaatctt ccatttgatc tgatggatac
     1861 caccttcttc ttggcacgcg atgcgattac tcctgcaaaa tctccaggaa tggctgtgtg
     1921 gcgcgagcgt ttatttgcca ccatgatgca aaacgcagct aagccatctg atttttataa
     1981 cattccagcc aatcgcttag tagagcttgg tgccaaagtt gagatttgaa attgatnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctgccacca acgatcaact ggagcttgaa
     2101 cgtttaaatc agattgagga agagcttact ctccaacgag agtgggctga ctaccgttgg
     2161 aagaaaacaa actctgagtg ctatgccaag ttttttgtga actcgtgcct gcaagatgcg
     2221 cgcgctaaat atcgtcgtga aattgacccg attcgcgccc aagaggtttt actcaatgat
     2281 aatcaacgca ttcttcggga ccgaattaaa actcagcgag atgctgagcg cgccgctgag
     2341 cgctccgatc caaagcgtgc ccaagaacgt gctgataacg agaaagcatt ccaacagaaa
     2401 caaaaagagg ctgccgcacg tgctgcggat cttgaggaac gccgtaaaga tgccccacgc
     2461 cgcgcccaag agaataaatc tggtgtgaag ttagattaga tctgtggcta agactaaaac
     2521 tctctatgtc tgccaagcct gcggtggcag ttcacccaaa tggcaagggc agtgtcccgc
     2581 ttgccaggct tggaatacct tagaagaatc gctttccgag gcatcatcag gtaatcgttt
     2641 ccaagggctt accaactcca taccgcggca aaaattagtg agcattaatg ctgaggattc
     2701 tccacgttta ccaaccggta ttgaagagct tgatcgtgtt ttaggtggcg gtttagtgcc
     2761 tggtggagtt gttcttttgg gaggcgaccc gggtattggc aagtctacct tgctgctaca
     2821 agccctagca gagctgagcg caaaaggagt cgatgtgctc tatagcagcg gtgaggaatc
     2881 tgctgcccaa attgcattgc gcgcaaagcg cattgtgttg gattcgcctt cgcttgaagt
     2941 gcttgcggag atccaattag agaaattgtt actttctgtg gaagctgcac agccccaagt
     3001 cttggtagtg gattcgattc aaactctgta ttccgaagcg ttttcatcgg cacccggatc
     3061 ggttgcgcag gttcgggagt gcgcagcaca gctcacgcgc ttggctaaat cgagtggtat
     3121 ctgtgttttg ttagttggcc atgtgaccaa agatggacat ctagcaggcc ccagggtgct
     3181 ggagcatatt gttgatactg ttttgtattt tgaaggtgat acccattcct cgtttcgctt
     3241 agtacggtcc atcaaaaacc gctttggtgc ggttaatgag ttaggtgtct ttgcgatgac
     3301 cgagcgcggt ctgaagggtg ttaataatcc atcggcatta tttttgtccc aacatccaga
     3361 aaccgttccg ggctcttgtg tgctagtgac ccaagagggt agtcgtccct tattagtaga
     3421 gattcaggct ttggtggata cggcgcatat ccccaatcca cggcgcttgg cagtgggctt
     3481 ggagcaggca cgtcttgcga tgttgttagc ggtcttgcat cgtcatgccg gtattgcctg
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctgtt ctcttggcaa
     3601 tcgcctcgtc gatccgtaat cgggctttgc caaaaagctt agtggttttt ggtgaggtcg
     3661 gacttgccgg tgagattcga ccatgccccc gcggtcaaga gcgcctcaaa gaagctgcca
     3721 agttgggatt ttcggtcgct attattccca aggcaaactt acccaagaca aagattgcca
     3781 atttacaagt gattgcggtt gagcgcattg atgaagctat tcgtgctgcg aatgatctga
     3841 gttaacaaga tctatttctg aagatcctct gccaaaatga tgcagacctg ttgatcacca
     3901 gcagatactt ctagccaaat cgctggtatg tgcggaaatg cctgatggaa gtattcatac
     3961 tcgttaccaa tttccagaac aagcgccccg cgttcattta agtaatcggc ggcgcccgca
     4021 ataattctcg tgatgatatc catgccatta agaccgcctg ccagagcttc cctaggctcg
     4081 gcttgatatt ctggggggag ggcgcgcata gaggcttcat tgacgtaagg gggattgcaa
     4141 atgatgagat caaaacgatg gtcctcgtcg gtatctggca gtgcattcca taaatctcca
     4201 tgtaacagct caatctgatt attcacatga tgacgctcaa cattacgcgc tgctaaagcg
     4261 agcgctggca aagaaagatc gcttgctgtg atgtgcaaat cgggatagtg aagtgcggtc
     4321 aagatagcta atgagccatt accagtgcac aaatccagaa ccttgccatc ggctggtaac
     4381 caaggatcta gttcgccatg gacaataagc tctgcaatga aggaacgcgg aatgatgctt
     4441 tgtggggtgc agtcaaatgg tatgcccatg agccatgctt cgccaagcag atatgccaat
     4501 ggctcacgac gactaattcg ttgttgtgca atgttgatgg catcttgagt catcttcgca
     4561 gaaaggggtt tatcgccaag atccagtgcc tcggttggag aaatacccaa ttgtttactg
     4621 acgatccaga gcgcttcact ttgcgcattg ttagcgccat ggccatagtg caaatgagcg
     4681 gactctagta attgagtaat ttttgtacaa cagtcctcca cggttaatac agatggagtg
     4741 aacgagttgg gggagggatc catagagctc acactaaccg agggtcagta agttgtgatc
     4801 aggcaataag tttttcgaga gtgcgacgat agatattctt aagcggctca atcgaattaa
     4861 gcgcaacacg ctcatcaatt ttatgactcg tcgcattgat gggcccaaat tcgacaagct
     4921 cagaacagat tttggcaata aagcgcccat cgcttgtgcc acctgtggtc gagagctcag
     4981 cgctcacatt cgtttcagcc atgatggcct cacgcatggc acctgctaga gcgccatcgc
     5041 ctgttaagaa aggctcaccc cctaatgtcc aagtaatctc aaactccaaa cctgcatctc
     5101 ttaagatact ctcaagcccg cctttgagct gttctggctt gctttcggtg gagaaacgga
     5161 aattgaagtc aagcgtgagt tcacctggta tgacattgtt agcacccgtg cccgcatgaa
     5221 cattagagat ctgaaaactg gttggctgaa aatactggtt gccacgatcc cactctttgg
     5281 taactagttt gctaagcgca ggggcaagct catgaatggg attgcgacct agatgaggat
     5341 aggcaatatg ggcttgtatg cccttgacct ttaattttcc ggaaagagaa ccgcggcggc
     5401 catttttaat catgtcaccc aattgattga cggaggtggg ttctccaatc acacagtagt
     5461 cgagtttttg gcctctttgt ttgagaagat cgcacacgat cacagtacca tcattggcag
     5521 gaccttcttc atc
//
LOCUS       DDPQ01000077            3840 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_40372,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000077 DDPQ01000000
VERSION     DDPQ01000077.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_40372"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2077..2221
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3200..3313
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1019 a          861 c          794 g          907 t
ORIGIN      
        1 aatggcgctg gtaaaagtac tttttttaat gttctaagtg gcgccttttc gccctctaaa
       61 ggacaaattc tttttaatgg tatcgatatt actcatcaaa aaccatacga atttgctcga
      121 atcggaattt cgaagagctt tcaaatcacc aatgtattta aacaactcag tgtccacgaa
      181 aacgttcgtg ttgctgccca gatgcaggtt tcgcgcttta acttcttaaa agatgcgcat
      241 tccctatcag gtaccataaa aagtgcagat gaacttctcg agcgcgttgg tcttcatcat
      301 ctacgccaca aaaaaacagg ggacttagct catggtcagc aacgagccct agaaatcgca
      361 atggcattgg cttgcaagcc aagtctccta ttactggatg agcctactgc tggaatgtct
      421 ccagaagaaa cgctggtcat gatggatcta attcgaacat tagctacaga acgaactgtg
      481 atacttgtcg agcataagat gaaactcatc atgggtttat gcaatcgtat tattgttttg
      541 catcatggcg agtttcttgc ggaaggcacc cccgatgaaa ttcagaataa cgctgaagta
      601 cgccgtgttt atttaggtca aggctagaga tgctaagaat cgagaacctg caggcctggt
      661 atgaccaaag tcatgttatc caaggcatct cattaaccgt aaacgcgggg gagattgtta
      721 ccttaatggg gcgcaatggt gccggaaaaa ccaccactct tcgaacccta atgggtcttt
      781 tatcgaaacg aaaggggcat gcaagtatcg atggaaaagc gtttttggag ctacctgcct
      841 atgagcgctt tcatctaggt attgcctatg tgccagagga taggcgcatc gttccaggct
      901 taactgttaa agaaaattta gagctcggta tgatcgcaca aaaatccaag ggtaatattc
      961 aggcgatgct tgatgaagtt gcggaaacct ttccaagatt aacggagcga cttcatcaag
     1021 acggtacctc aatgtcaggg ggcgagcaac agatgttggc gattgcgaga gcgatggtag
     1081 cgaagcctaa gataattctt ttagatgagc catctgaagg aatcatgcca gttttagtag
     1141 aagagatgtt cacactgttt actaaaatga agcaagcagg aatgaccatc ttattggtgg
     1201 agcaaaatgt tcaaaaggct cttggtatat cagatagagc atatattctt gatcaaggca
     1261 aaattgtttt tgaagactct gcaaaagcac tgcttaataa ttatgagatt cagcaaaaat
     1321 attgttcggt ctagatttac agtaaaccca aggctcttcc aatctcctcc caaaattctt
     1381 ttggtatttc tgtcagcaac cacgttaggg ttatatagcg caacagtttt ccaatcgcca
     1441 tgtaaatcaa gcactgcaac caattaaagc gtagccagcc ggcgaccaaa cacaaaggat
     1501 cgcctattgc gggcaaccaa gacaataaca acatgggtgg tccccgcttc tccagccaga
     1561 cttgtgcccg agaatttgtc tttcccttta gagattcgta ggtattgcgt gccaagagtc
     1621 ctagccacca attaatcatt cccccaatcg tattgcccac tgtagctaca gcaattgcca
     1681 accagtagtc atccggcttt aagctaacgt aagcaaataa aacaggctct gaacccattg
     1741 ggattaaggt ggctgagacc attgccacaa taaatacagc gggcaagccc acagtgggca
     1801 ttccaaacca ctgcaataga gtctgaatcg cagcttccat taatgaagga tgcccgcttc
     1861 ttttaaagct ttaatttgct ctgcacttag accgagcgat tgcaaaatga tgtcggtatg
     1921 ctcacccagg gttggggcac gatgacgaat agctccagga ttgttagata gtttaggaac
     1981 aactccgggc atctctactt taagtccgcc cgcgctctca accgtctgaa tcacacctcg
     2041 ggcacggtaa tgcggatcct cagcaatatc tttagcnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nccaattaac tccattagac gtttgaacaa agaatcccca ttggctgcaa tcaaaacgta
     2281 ttgcccgtct aagcaaggat aggcattgga tggtgcgatg cctggtaacg cacccccagc
     2341 tggttggcgc accgctccaa agacatggta ttcaggtaac aaactctcgg ttaagttaaa
     2401 caccgcctca tacaaagcca cgtcaatcat ctgaccttca ccgcccctgg catcgcgctc
     2461 atagagtgct agcaagacgc caagggcgcc atgcaggcct gcaatcgtat cgcccagaga
     2521 taaaccggct cgcgctggta cctcgccagg atgtcctgtg atgtagcgaa gcccagctgt
     2581 tgcttctgca atcgcggcaa agccaggctc atcccgtcgc ggtccatctt ggccgtatcc
     2641 cgatacacgc agcatgatga gcttaggatt aagcgtatgc agttcatccc aacccatgcc
     2701 ccacttctcc atggtgccag gtcggaagtt ctctatcagc acatcagcct ctttggccaa
     2761 acggcggacg atgtcttgac cttccttcac tcgtaaatca atgcagatgg attgtttgtt
     2821 acgggattgc gcctgccacc atactgaagt gccctcatga atcatgcgcc acttcctcag
     2881 aggatcacct tcttgagggg attccacctt aataatctcc gccccaaaat cagccaaggt
     2941 cttggcagca aagggtcctg caattaattg ccctagctct aagacacgga ttccagaaag
     3001 aatttgatgc attccaatca gcccattaaa caaataagta tctagcttaa actattgata
     3061 tcaccaataa attaatcaac taagttctta tgactcaccc cattccaaag cccgaccaat
     3121 ttcaagagat gcgcgaggca ttacgggatt tatgttctcg ctatgactct gcttactggc
     3181 aacaagtgga tcacgaacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnatcatgg aagagattaa tctctcaggc ggtaatgcgg gctcatgcca
     3361 tggtcagatg tataacatgg gcaccttgct gcgccacggc tctgatgagc aaaagaaact
     3421 atatctaccg aaggttgctt ctggtgaact tcgcctacaa agtatggctg tgaccgagcc
     3481 aaccacgggt accgatacca ccaagctcaa gactactgca gtcaaaaaag gtgatcgcta
     3541 tgtaatcaac gggcagaagg tatggatctc gcgtattcaa cattctgact taatgatcct
     3601 tctggcacgt actactcctc tagcagatgt caagaaaaag tcagagggca tgtccatctt
     3661 tatcgttgat cttcatcagg caattggtaa gggaatggaa gttcgtccga ttgcaaacat
     3721 ggtgaatcat gaaaccaatg aggtgttctt tgataatctt gaaatccctg ctgagaactt
     3781 aatcggtgtt gagggtcaag gattcaaata cattctggac ggtctcaatg cagagcgcac
//
LOCUS       DDPQ01000078            4229 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_14160,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000078 DDPQ01000000
VERSION     DDPQ01000078.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_14160"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3596..3730
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1104 a         1004 c         1012 g          972 t
ORIGIN      
        1 acatactcac gcgtctctcg tggcatcgtt aagctgagat agtctgttgg cttacgttgc
       61 gcctgattgc gcttgatggc tttagcaaca ttgccctcac cccagttata ggctgccaag
      121 gctaactgcc aatcaccaaa ttgattgtaa agtcgttgca aataatcaag tgctgcatta
      181 gtcgattgca aaacatcgcg gcgctcatca cgaaagacgt tttgggttag cttgaaatcc
      241 ttgcctgttc tgggcatgaa ctgccaaata cccattgcct tagcggttga ctttgcctcg
      301 ggattaaaag cgctctcgac aaagggtaat aacgctaact cgcttggcat gttgcgacga
      361 tttacttctt ccacaatata gaagaggtaa cgcgaggaac gcgccattga gcgatcgagg
      421 taatcagggc gcgctgtaag ccagcgggtt tgctgaagga caagaggact atctaactcg
      481 ggtaactgaa atccctgacg aatatggatc cataaattat tggagggagc cattacatta
      541 ctaaccgact gtttactcaa atcaacgcgg ggcgccttag cagctcgcgg gtcgcgcttg
      601 gtatcggcag taggagacca ttcattccca gtgctagcac aagcactgag caataaaacc
      661 agtgcactac tcacaatagt taggtagcga ctcaagttca tcttagaagc gatccttcca
      721 ggcgcgcacc accgctaaga cctctgctgg agtattcagc ttcttgccaa tcacattttc
      781 tgcagcagcg aggacaggct cttggtcaca gcgcatgaat ggattaacgc gtttttcgtg
      841 agcaatgttt gttggtaccg tgggctgatt acggtctcgc aactgctgcg cctccttagc
      901 ccacgcttgt aagttgacat tctggggctc tactgcgagc gcaaaacgga tattcgataa
      961 ggtgtactcg tgggtgcaat acaccaaact atcctctggc aaggataaga actttgccaa
     1021 ggaagatgac atttgggtgg gagtgccctc aaataaacgg ccacagcctg aggcaaataa
     1081 ggtgtcccca caaaagagct taggggtctc gccggggtta tgggcagtat cagccacata
     1141 ggcaatgtga cctaaggtat gccctggaac ctcaagtacc ccaagagaga ttgcgggctt
     1201 agcaaactcg acccgatccc cctcgaataa ggcatgggtc cgctctggga tgtcctctgc
     1261 agcagggcca tagatcggaa acgagaatcc ccgagaatgc ccccacgtca gtaaatcctt
     1321 taagccgccg atgtggtcag cgtggtaatg agtgattaga atgccactaa ggtgaaggcc
     1381 ttcttgctga aggtagtcaa tcgctggttt agcatctcct ggatctacca ggatggcaga
     1441 ttggctgtcc cgaatgcacc agatgtaatt atcagtaaag gcaggaatgg gccaaacgtg
     1501 taaggtcgaa tttgaagcca ttagcaaacc cttgggacac aagtcaatcc ttctatgata
     1561 acaagcactc cccccgatct gctgactgac cacccccccg agaactcttg ggaaacctgg
     1621 ctgcaatctc cgcctgggca atacatcttg caatcagaac agttgctctt taatcaagcg
     1681 gtcagcgatg tgtttggtta tcacgctctg caaatcggcc tgcctcaaat gaacacgctt
     1741 tcagaaagtc gtatttctct gaaattgatg ctattgcccc atggggtttc taaatcagga
     1801 ggtgaattac cttaccaaca aatcgaggga ataccagaag agttgccttt tgcaaatcaa
     1861 tcgattgatt tggtgatcct gccccatgtg ttggagtttg cgaacgatcc gcatcaaatc
     1921 ttacgagagg tggatcgcgt tttaatcccc gagggtcgag tagtaatctc tggatttaat
     1981 ccagcaagcc tgtggggtct gcggcaatat accgggcgcc tgattggtca agcctacctc
     2041 cctaaagaag ggcagtttat gagtttgctg cgggtaaagg actggctcaa gttattagac
     2101 tactcggtcg atcggggtca ctttgcctgc taccgtcttc cggttcgcaa agaaaagggt
     2161 ttggcccgca tggcctttct ggagagcatg ggtaatcgtt ggtggcctat ctttggttcg
     2221 gtctttttaa tctccgccat taaacgccat gcgggtaccc gactagtagg tcgcgttcct
     2281 aagtctgcgc tacaagccct accgcaattt aatcctgctg cccaaaatgc cagtcaacaa
     2341 agctcagaac aggtataaag acgtcatgag caaacctcac atcacaatct ataccgatgg
     2401 cgcatgcaaa ggtaatccag gccctggagg ctggggtgct gtattgcgtg ccggccatca
     2461 tgagaagcat ctgcatggcg gtgcgcactc caccaccaat aatcggatgg aaattagtgc
     2521 agtaatccat gcactcaaag ccttaaagca agccagcgtg gtcgaactct atacggactc
     2581 tcagtatgta caaaaaggcg taaccgagtg gcttactggc tggaaggcgc ggggttggaa
     2641 aacagcagat aaatctccgg tcaaaaatgt cgatctctgg caagagcttg acgccctcct
     2701 acctgatcat gagatcgcat ggcattgggt acggggccat aacggacacc ctggcaatga
     2761 attagccgat caacttgcca atcgcggggt cgaggagttc ttaagcgcca aataaaccca
     2821 tggcaggcta aaccgacaca gacatatcgc ttgtgagaga atggtttaac tctataaata
     2881 taaaagctat ttagaccaac gctgtgactg aaaaactatc caaaatcgag cggctcaaaa
     2941 agaaattgat gtcttggggc tccgctctac tcgctcgtat tcagagtatt gatctaaaga
     3001 atgtcgcagc ctttgtgatc aaacacaagt ggcgcatcct aatcgttctc attttgatct
     3061 atgcaggcaa taaagcctac gatcattttt ttccgactga ggttaaacgt ggtggggtgc
     3121 aaacagtcac cacaatggtt ctggagaaaa aagatgtnng accgatgatt acaaacacgg
     3181 tcaaagagat ccatatcaaa gaaggtcaag atgtgaagaa aggacagctt ctcttcacct
     3241 tggatgaccg taatgatcgt gctaactatg acagggcgaa agcactggcc gatgatgcac
     3301 agcgtcagtt acaacgtgcc aaagaattgg tagctaaaaa ctttatctcg aaagcgggtc
     3361 tagacactgc agaggttaat gctaaatcag caatggccac cgcgcgtgca gcggaggctc
     3421 agctctcctt tgatagcatt cgctctccaa tcgatgggcg ttccggcatt atcaatgtgt
     3481 tcccaggctc acttgtgcag gcgagtaatg tggtggtctc cagcaccacc tcaaccgcta
     3541 cctcaacgac aggtgcaatg gtgacaatta cgcaaatcga cccgattaat gtgcannnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn caatttgcac ggattaagct agaagcaaat accctaaaag acgccattgt
     3781 gattccgtcg caagcagtgg tactcaatgc caggggtcgt tttgtatacc ttgttggtca
     3841 agacgataaa gtcagtctaa agcccattaa ggtcacttat gagtaccaag gcaatgcagc
     3901 gattactggg gtcgaggttg gtgagcgtat cgtcatcgaa gggaaacaga acttaaggcc
     3961 cggagtcaaa attagggagg ctaaagtaac tccacctaac aaacctgccg caaccgaatc
     4021 aaagcctact gatgtaaagc cctctgaagc aaaaccctca gagtcaaaac ccgctgagaa
     4081 aaaatgacgc tctctgagct atgtatccgc cggccggtca tgaccgtact gctatcggtg
     4141 gtcacggtgg ttgctggagc aatcgcctac acacgaattc cagtagcagc gctccctagc
     4201 tttaatacac cggtcatctc ggtatctgc
//
LOCUS       DDPQ01000079            3936 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11038,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000079 DDPQ01000000
VERSION     DDPQ01000079.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11038"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2502..2615
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3285..3439
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          968 a          799 c          960 g          938 t
ORIGIN      
        1 ttgatgggta tgggtcagat ggtgaccatt aatcaagtgt tggaccaaga tacagcgatg
       61 atcatcgttg aagaaatggg ccacaaagcg catgctgcca aattagatga ccctgaaatt
      121 gatttgggtg cttcggcaga agatgcaccc tctttgccaa gaccgcctgt cgtaacggtg
      181 atggggcacg ttgatcacgg taagacatcc ttgcttgaca aaatccgcgt tgcgaaagta
      241 gcggcgggtg aggctggtgg aataacccag catattggtg cctatcatgt ggagactcca
      301 cgcggggtta ttacattctt ggatacaccg ggtcacgagg cctttacagc aatgcgtgcg
      361 cgcggcgcca aggcaaccga tattgtgatc ctagtagtgg ctgccgatga tggtgtcatg
      421 ccacaaacgc gcgaggcaat ccatcatgct aaagcagcta atgtcccatt ggtagttgcg
      481 attaataaga ttgataagcc agaagcaaat gctgataggg ttaagactga attggttagc
      541 gagcaagtcg tacctgaaga atacggtggc gattctccat ttattggcgt ctctgcaaaa
      601 actggtgaag gaatcgatgc gctattagag aacgtactgc tgcaagcaga aattctggaa
      661 ctcagagccc ccaaagatgc gccagcacag ggcattgtga ttgaggcacg cttggataaa
      721 ggccgtggac cagtagccac tgtattagtt caatctggca cattaaagcg gggcgatatg
      781 cttttggctg gccagtctta tggtcgagtg cgcgcgatgt tggatgagaa tgcaaaggca
      841 accaacgaag ctggcccttc cattccggtg gagatccaag gtttatcgga agttcctgat
      901 gcaggagagt cattccaagt cgtggcggat gagcgcaagg ctcgtgagat tgctttgttc
      961 cgtcaaggta agttccgtga cgtgaagttg gcaaaacaac aagccgtcaa gcttgagaac
     1021 atgatggaga atatgggcga aggcgcagta gaagccaagc tcttgcccat cattattaag
     1081 gcggacgtac aaggttctca agaggccctt tcgcaatcct tacaaaaact ttccaccgat
     1141 gaggtgaagg tgcaaattgt tcatgctgct gtaggcggta ttactgaaac ggatgtgaac
     1201 ttagccattg cctctaaagg cgtgattatt ggatttaatg cacgtgctga tgcggtggct
     1261 cgtaaattag cagagaccaa tggtgttgat attcgttatc acaacattat ttatgacgca
     1321 gtggatgagg tgaaggcagc cttaagcggc atgttaaccc ctgataagaa agaagaaatt
     1381 atcggtatgg ttgagatacg ccaggtcttt aatgtatcca aggttggcgc tatcgcaggt
     1441 tgtatggttt tggatggcat cgttcggcgt aactcacgtg ttcgcctatt acgcgataac
     1501 gtcgttgtgt ggaccggcga gctagattcc ttaaaacgct ttaaggatga tgtcaaagaa
     1561 gtaaagggtg gttttgagtg cggcctttcc ttaaagaaca gcaacgatat caaagagggc
     1621 gatcagcttg aggtctttga ggttaccgag gttgctagaa cgctttaatt gattgctgta
     1681 tgcataaaac aagtccccat cgtcaccaac gtctcgctga tcaaattcag cgagatttgg
     1741 ctgagctcat tccacgagaa ctgagggatc ctgccaatgg cttaattacc attcaggcag
     1801 ttgaactctc ccccgatcta gcccatgcaa agatttattt cactgttttg ggtggggatg
     1861 cccaatatgc attgggatct ttgcaggata aggcgggcta tcttcattca ttactcttta
     1921 aacgaatgca tacccatact gtgcctactt tgcattttgt ctttgatacc tcaatcgagc
     1981 gtggcgcaga aatgtctcga ttaatagatc aggcattagg ctcaaagcct aattaattac
     2041 gatggctcag cggattgacg gggtggtctt gttagacaag ccctccggaa tgagttctca
     2101 aagtgcggta acttccgtaa aacggctact gcatgccgag aaagcggggc acactggcac
     2161 gctggatcca atggcaactg gactattgcc agtttgtttg ggtgaggcta ctaaatactc
     2221 gcaagattta ctagatgcag acaaggtata ccaagcaaaa ttacggtttg gcattcagac
     2281 tgatacgggt gacgctcagg gccaaatcat cgctgaaaaa tcaacagcgg aagtcacgga
     2341 taacgctatg gcccagggat taatggaagg aattttgcct cgttttactg gtcagattga
     2401 gcaagtgcct ccgatgtatt ctgctttgaa gcgagatgga aagcccctct acgagtatgc
     2461 acgctctggc attgagatcg agcgcgagcc tcgtaaaatt annnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaggc cgctacttta gagaacttgc
     2641 agcatacctc cccccaagtc ttaccagtcg atgcactatt acaaaccttg cctgagctga
     2701 cggttgatga tcaccaagca aagcgcctag agatgggaca acgcgttcca tgcgctttaa
     2761 atatcgctac acagtgggct aactctttat ttcggattta tcggagccaa gcgcttcctc
     2821 aaaactttat tggtactgcc gattggcgct cgggnngcgc cttatcaacc aaaactaatt
     2881 tctcaccttt attaagcaaa gtatcaaatg accaaacgcg cacttcgtaa tatcgccatc
     2941 atcgctcacg ttgaccatgg caaaacaact cttgttgacc aactcttgcg tcaatcagga
     3001 acttttcgta ccaatgaaaa gctctctgag cgaatcatgg actccaatga tcttgaaaaa
     3061 gaacgtggca tcaccatttt ggccaagaac tgtgccgtgg agtatgacgg tacgcacatc
     3121 aatattgtgg atacccctgg acacgcagac tttggtggcg aggtagagcg tgttttatcg
     3181 atggtcgatg gcgtgctctt gttggtggat gcggtcgagg gcccaatgcc ccaaacccgt
     3241 tttgtgacca agaaagccct tgcactcggt cttaagccta tcgtnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnt caattgatta cagcagctat gtcggaaaaa ttggagtagg
     3481 ccgcattagt agaggcagga ttaagcctgg tacagatgta gtttgtgtga acggtccaga
     3541 tggaactcca tttaagggcc gcataaatca agtattgaag ttcagaggtc tcgagcgtga
     3601 agtggttgag gaggctattg caggtgacat tgtccttgtc aatggtattg aggacctagc
     3661 cattggcacg accatttgtg ccccagatcg agtcgatgct ttgccaatgt taaagattga
     3721 tgagccaacc ttaaccatga actttatggt taataccagc ccacttgctg ggcgcgaagg
     3781 gaagttcgtg accagtcgtc agattcgaga gcgcttagat cgtgaactta agtctaatat
     3841 ggccttacgg gttcgagaga ccgatgatga cactgttttt gaagtatccg gtcgcggaga
     3901 gctccacctt actattttgg ttgagaacat gcgccg
//
LOCUS       DDPQ01000080           16387 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_6723,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000080 DDPQ01000000
VERSION     DDPQ01000080.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 16387)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16387)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16387
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_6723"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    123..183
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8568..8655
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10389..10434
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15311..15418
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4128 a         3877 c         3659 g         4414 t
ORIGIN      
        1 taaaaataaa gtatttgcag cacttgctgc ccatggccca actccccgct tcacgctaaa
       61 gatggccgaa caatcggtgc cgatgttgat cgccgcagcg atagagctag aagaaagtct
      121 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnngtgcctt ctgagcagat tgaggaaaca cataaagcct atgaggctgg cgcgtcattg
      241 gtgcatattc acgtacgtaa tcccgatgaa agcccaagct ctaatcctga cctatatgcc
      301 gaagtgcaag agggagttaa aaaatattgc ccagaaatga ttgtgcaatt ttccactggc
      361 ggacgtggtc gtgatcaagc ggctcgtggc gccatgctct ttcatcggcc cgatatggct
      421 tctttagcaa ccggctccac aaactttcca gttggcatat atgaaacnnt atgaaaaccc
      481 taccgatttt gttgagggtc ttgcaggaga gatgctgaaa tatgaaatta agcctgaaat
      541 agaaatattt gatttagcga tgctgtataa cgcagctaac cttattaaaa agggactatt
      601 aaaggcccct cctcatgtcc agtttgtgat gggtgttcca aatgccatgc ctgtgcggcg
      661 atcgatctta gagtttttga ttaaagagct taatgatgtc atgcctaccg ctacctggac
      721 tgccgcaggt attggtcgtc accagctaac ggtgaacgaa tgggcattag agcttggtgg
      781 tcatgtacga accggtttag aggataactt gcgctatgac gaaacacgcg tggcaaaaga
      841 caatgctgag ctggtagctc gcgttgcaac gttagcgcgt aaccaaggtc gcacagtagc
      901 gagtggcaaa gaggctcggc aaatcttggg gctaaggcag gccgcttagc gtgatttctt
      961 ctgctgccaa agagtttgat tacgttattg tcggtgccgg ctcggcaggt tgcgtactag
     1021 ccaatcgact atctaccaac ccaaatcatc gtgtttgttt gattgaggcg ggaccaccag
     1081 atcgctctcc atggatccac ttgcccattg gttatggcaa gaccatgtgg gatcccaagg
     1141 ttaattggaa atttaataca gagccagacc ctggtatgaa cggacgccat atttactggc
     1201 cacggggtaa atgtttgggg ggatcgagtt ctatcaatgg cctcatcttt atccgtggtc
     1261 aaaaagagga ttacgatcac tgggcatcac ttggcaatga cggctggtct tgggatgatg
     1321 ttctgcccta ctttaagaag gctgagggaa atgatcgatt aggtgcgcct ttgcactctc
     1381 aagagggccc cctgcgcgcc tcttccatca agaaaaaaca cccgctcgta gaggcattta
     1441 ttgctagcgc caatcaattg ggcgttccca aaacagagga ctttaataat ctagagcaag
     1501 aaggtgtggg atatttccaa ttaagtactc ataatggttt gcgttgtagc gctgccgttg
     1561 cgtatttaaa gcccatacgg tctcgcaaga atctgaccat cattacggat gcacaggcta
     1621 gtaaagtgct gtttgaggga cgcaaagccg ttggtattga atttatccaa gcggggcaac
     1681 gccacaatgt caaagcccat catgaggtat tgcttagtgc aggtgctatt caaagcccac
     1741 aactattgca actatctgga attggccctg ctccgttact ccaagagttt ggtattccgc
     1801 tactgcacga ctcccctgga gttggcgaga acttgcaaga ccatttgcag tatcgcttga
     1861 tatatgagct taatcagaat atttctacca attcacagat ctcttctctt tttggaaaaa
     1921 tgcaaatcgg tcttgattgg cttctctttc ggggcgggcc gctttcgatt ggaattaatc
     1981 aagggggctt gtttactaag gtcatgccac aatccaagac ccccgatatt cagtttcata
     2041 tggctacatt gtctgcggat atggcaggcg gccaagtaca ccctttccca ggatttacca
     2101 tgtctgtctg ccaattaaga cctgagtcta gaggctatgt tcgcattgca tcgcgcgatc
     2161 cgctagctgc ccctaagatg atggctaatt atttatcaac ccagcatgat cgcgatacca
     2221 gtgttgcggc tgtaaagttt gcacgtcaaa tcgctcaaac cggccctctc aagaatttaa
     2281 ttcagcgcga gattaagcca gacaatcccc agtctgacga agatttgctc cagttttgcc
     2341 gtaataatgg cgcaactatt tttcatccaa ccggcacatg tcagatgggg cctgatcatc
     2401 agactaatgc ggttttggat actaagctac gagttagggg cgtaactaat ctgcgggtag
     2461 tcgattgctc ggcgatgccc accgtaccct ctggtaatac taattggcca gcagtaatgt
     2521 taggcgagaa agcgtctgac cttattttga gtgagcggaa taaacgttag gcgtcaatta
     2581 aatccagtaa tttcaagcgc tgtacttttc cagaggcgcc ccgtggaaga tccctcacaa
     2641 acttgattac tttaggggtt ctaaatttac ccagagtttt gatacaaaag tctcgcatct
     2701 ctacttcact acagctcacg ttatctttca aaataataca agccataatt tcttggccat
     2761 agtttgtatc cggtatacca accgctgctg catctagtac tgctggatgc tttagtagtg
     2821 cttcatcaat ttctcgagga gcaatattct ccccaccttt aatgattaat tctttcaggc
     2881 gccctgtaat aaagtagaaa ccatcggtat cgcgcatacc aagatcacct gtttttagcc
     2941 aagcatctcc cccaaatgct ttttgggttt cagcttctcc tttgtaatag gcgctcatta
     3001 cattattgcc acgcaaacat atttcaccaa cagtttgatc gccaagcaca agcccactag
     3061 gatcaatgac ctttgcctca actccacagg gtaagcctgg actgccatac cggcggcgtt
     3121 cgtcgtgagg attgcagaac accattgagg acgactcggt catgcccatt gcttctatga
     3181 cggtaatacc aaataaagcc tcaaattctt tatgttgctc cggtggcaaa ggagctgatg
     3241 ccgaccgacc aaaacgaatc agttttagat cttcgcgagt aggtttcttg ccagttgatt
     3301 tggctgcatt gattaagtaa gcgatgatgg ttggaaccat attaatccag gtggcttgat
     3361 atttttgtgc caaatcccac cacgcactta ccgaaaagtg atgtggagca ataatcccgc
     3421 caccggtata aaacggagta actgttgata tgcattgacc attaatgtga taaataggta
     3481 aggagcttag aactacatcg ttttggtcga gagaatgcca ctcccctatg gatttaccag
     3541 cgtgcattaa attacggtgg cttaacagaa cacccttcgg cgtgccggta gttcccgagg
     3601 tatacattaa caatgccgaa tgattttcag taatttcagg tagcgttatc gctgctgtat
     3661 ccatgtaagg accaacagcg gcatctggat caagttcaac catcatgaac ttacgtttcg
     3721 tatgaggtaa tgcggctagt aaagcatctt tatttagggg ggcataaaag agtatttcaa
     3781 tatcgctatg gtccagtgaa tacgccaact gctcaggcgg tgctagcaaa ttaaagggtg
     3841 taatgacacg acccccaacc atagtcccca aaaagagtcc tgtcgtttgc cgcccattgt
     3901 gcataaaaag accaatatgg ccgtgttcac tgatctttaa ttgatcaaac caagcatgta
     3961 gatgctgggc ctcatccagt aactgctggt aagtaattct tagattgggc tctggggcaa
     4021 ataacaagac ccgatcaggc tgttgccgag cccaataatt taatacccgc tgaagatgaa
     4081 ccgcttcctt atgcaatcga caataccgaa attatttagg aaagcgctga aaatatgtct
     4141 cgagtaaatc ctcaagcctg gggacttttt ctggaatcac ccgcacaact ctagaaatat
     4201 ttaagtaatg gcggtagttc atgttctcag aaaaattatt tttaccccag gtaccacagc
     4261 ccatcgataa ggagaatggc aggccattat caaaactacc accggtagca atggcatgag
     4321 cttggttcac aatcacacgg gccaccggaa gctcttgcgc taattgcgcc gcacgatcct
     4381 ccataatctc tccctttgct gctgtatgaa gccccaccga atgccccgcc ccttgatacg
     4441 agtagatatg agcaaggagc ccttttgcct tattaaaatc agaagcacgc cacacagtta
     4501 gtacaggact tagcttttct ccagaaaatg gtgcagacga tccatatccc gtctcctcaa
     4561 ccataaagaa cttagcatcc ttggctttgg ggtgtaaaag accaacttta ttagcaatat
     4621 agttagcgga ttgagcagtc agagttgatg tgagtttacc gttctgaaac ataatttctt
     4681 gcaaacgctg tttatcagca gaatcaagca tcacaccacc cactgcttct agagccttaa
     4741 ttccagcatc atagatagag tcaatcaaga taaggccatt ctcactagag cagctagtag
     4801 cattatcaaa tgtcttggat actaaaattt tctgtgctgc taaggcaaga tcagcatgnn
     4861 ccacttgtat atgctgcacg aatattggcc tgtgatccgg ttgccaccac taaatcagct
     4921 aaacgcatga gttcttgcgt tgcttctttt gtaatcggtg ctggtaagca ttggactaaa
     4981 tcacgtggcg ctcctagtaa ttctaattgc gcatggacaa attctaataa tcgcacacag
     5041 gtgctttgcc cctttggcga aggcgctacg attatcgcgt tgcgaccctt aagcgcattc
     5101 agaattttat taatcggggt tgcgcctgga ttagtagacg gagtaattgc tgctacaacg
     5161 ccaacgggcc ttgcatattc actaacacct gtctcaggat tatgggcaat tagtcctacc
     5221 gtttttgcag cattaagatc acgaacaagg cctaaagttt tgcggtaatt cttcttaacc
     5281 ttatcctcca catcaccaag acctgtatcg gcaacagcaa gttcggctaa ctcgcgattg
     5341 cgacttggct ccaaaattgc ccaggcaaca gcttgggtaa gcagatcaat ctgatcttgt
     5401 gtatatgaat cgagcaccct ctgtgcggag cgtgctcgag tgactagatc aacaacgggt
     5461 gagactgtac tggtattcat tattctgctt taatgccatt ctctttcaca accttatccc
     5521 acttaacaag ctctgctttt acaactctac ccagtgcctc cggtgagcca ccaacagctt
     5581 ctgctccttg attgagtaat ttctctttta cctcgggtgt ggttaatacc ttattagcag
     5641 cggcatttaa gcgatcaacg gcagcctttg gtgtattggc gggaacaaac atggcatacc
     5701 aagaatccac ttcaaaatct ttaaagccag attcgatcat ggttggcacg tctggaaata
     5761 cggcagaacg ttttgttgta gaaacagcca aggcttttag tttgccagcc tttacaaagt
     5821 tagctgctgc tggtatcgat acccaaagta atggtacctg accgcccatc acatcagtta
     5881 cagcaggacc accaccacga tatggtatgt gagtcatctt cacattagcg gcaccagcca
     5941 tcatggcacc cgctaaatgt cccggggatc cattgccaac cgatgcatag gcaatcgtac
     6001 cgggctttgc ttttgccata tcaatcacgt ctttgacatt cttggcagga aaatctgggt
     6061 tagcaaccag tatttgtggc aaggatgcaa tggtgaccac tggcagaaaa tctttttcag
     6121 tattgaaaga taacttagga taaattgcag gattgatggt gtgtgaagat aaggtaaata
     6181 aaacggtata accatctgca ggtgagcgtg ctgctgcctc gctaccaatc gatccacccg
     6241 caccacctcg gttctcaata attacttgtt gtcctagatt tgagctcaag ggttcttgca
     6301 caatgcgcgc aatcacatcc gtaccaccgc ctggtgggta aggaactatg aacttaatag
     6361 gcttattggg gtaacttcca gactgggcaa gcgccatgcc tgcccagagc gttgttgctg
     6421 caattaaaaa ctttaatcct tttttattgt ggatagcgtg catcatatgt ctccttatat
     6481 gattttataa acagaattta tacaaacgat ttatatttct ctaaatgcct tactggttta
     6541 cttaaagcat cgcgacggaa tgggtcacct agttccttgg ttaccatcat ctcaatcacg
     6601 gttgttttac cttccgcctg atttttaact gctgtttgta tagcggcacc tacatcaccc
     6661 actttatcca cagttacacc ctcgcaacca aaagatttag caacggcagc ccaacttgga
     6721 ttaacaagct caaccccttg atagcgacgt gaatagaaat caacatggtt cttcttctca
     6781 gcgccccact gtccgttatt aaatagaata acggtaacac caattttctc gcgggcacag
     6841 gttaataatt cattaagact aataccccat gcaccatcgc ccacataggc aaagacagga
     6901 cgatttggtg aggcaatctt ggcgcccact gctactggaa aggcataacc gcagttacca
     6961 aagctcatag cggcaagcat ggatctgggt tcatcaaagc gcaagtatga attggatacc
     7021 gagcaaatat taccaatatc ggtagaaacg attgagttct ttggtaaggc attctcaatg
     7081 gcgcgcaaca tctcacgcgg atgcattact ttgctgtctt tgatcatatc gattgaatat
     7141 ggatctttct catgagtcca agaagtcagt tcttcctccc atgcattttt ttcacccttg
     7201 atagtggtat cacgctccgc cttattcttg atacaggcta catcacgtgt ctttaattta
     7261 gccaagagtg cagcggcagc ctctttggca tcaccattaa taccaaccga tattggcttc
     7321 actagaccaa gcatcttgcc atcagcatca atctgaataa tcttggcatt cttaggccag
     7381 taatcaagac catgttgtgg caaggttcca aacggtccaa ggcgggtacc cagagccaat
     7441 acaacatctg cctttgcaat taacttcatc gcagctttcg atccttgata gcctaatggg
     7501 ccaacccata aaggatgtga ggcagggaaa gaatcattat gcaaataact atttgctact
     7561 ggtgcatgca aatattcggc aagagccaca gcctctttag tgccgccaga cataatcaca
     7621 ccgccacctg ccagaatcac aggaaacttt gcttttgcca atagatctgc tgcatcttct
     7681 aaggctttct cactaccagc accaagcttt acacgaattg gcttcggaat ctcacactca
     7741 atatcgccat agaaaaaatc gcgtggaata ttaacttgcg ttgggcccat ctcgagtagc
     7801 gcacgatcaa acgcacgtgc agtaagctct gccattctgc gtgggttcat cacatgcgct
     7861 tggtatttag taatttttga gaagatgggc agttgctcgg tctcttggaa tccaccgagg
     7921 cccatagtca tcgagccggt ctccggagta atcattacca cgggtgagtg ggcccaatac
     7981 gcagctgcaa gtccagtgac aaaattggta acgccaggac cattctgacc aatacaaaca
     8041 ccatgacgtc ccgatacgcg cgcatagcca tcggccatat gcactgcgcc ctgttcatgt
     8101 gcgactggaa taaaccgaat acctgccgct ggaaatatat cgagcgcatc cataaatgct
     8161 gaccctacga tgccgaatac atcggtaacg ccttgcgaga ctaaggtctc aataaatgct
     8221 tccgaaggag tcatcttttg aacaccttca acaacgattc ggtttggatt ggacatttga
     8281 ttcatggcta gctccttaat ggggtttgta tttatctcac tatattaata gcactctacg
     8341 ctatagtaat ccatctcatt aaactgaaca acttgtttca tattagtata aatactaact
     8401 aataatgcta tatttattga aatgttcagc taaaatgaac aaattcatta atttattagg
     8461 attaattgag atatgagtga gatcgatacc ctagagtttg gggaagttgt aaagaaagac
     8521 ctcggttctg aaaactcctc gatgcttcgg ggaatctcaa tccttgannn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnntgcat cgtacattag caatcttcga agaagcgggc cttgtggttc
     8701 gtgagcctgg tggaaaaacc tacgcaccag gggagcgcct ttcccgtttg gctcttgaga
     8761 ctttatgtca cgacagtgtc tccgcaattc gtcatggcat cttgcgtaaa ctcgttactg
     8821 atttaaaaga aacctgcaat ttagccatcc tccgtcgagg agagcttttt tacttggatc
     8881 gagtcgaggc taattggcca ttacgtttgc atttgccccc tggtacggtc ttaccgcccc
     8941 actgcagtgc gagcggtaaa ttactcttgg cctataagcc aaaggatgag cgcattaaat
     9001 tagtagacac attacccctt gagcaattta ctcatcgtac gattgtggat cgcgacgtcc
     9061 ttctatcaga acttgatcgg attgctgcta atggctatgc ggttgataac gaagagtacg
     9121 ttttaggcgt gtcatgtgtt gctgttcctg taaaagatca gtcgggcgaa gtggttgcag
     9181 cgattgcgat tcatgccgct accgcgcgct tgcccctaaa tcaggcgatg gagtacatac
     9241 cgaagctatt gcttgcggcc gagcaaatca gcgcaaccct ccactaagta tgaccaagcc
     9301 ctcttaccca catctacaac aactgattga cggagttaaa actcagacaa aactgggggt
     9361 ggtttatccg ctcagtatcc cagctcttga gacggttgcg catattgttg cgcatcaact
     9421 ctgcataccg gttttaattg gtccagagga aaagatccaa cagcttgcga aagatgcgca
     9481 gattaatcta gccggtattg aaatcatcaa tacgtcttca gatccgattg aagcagccaa
     9541 gaaatctgtt cagcttactc gggaaggtgt ttttggagcc cttatgaagg gctctttgca
     9601 tacagaagag ctcatggcat ccgtagttag tcgcgatggc ctgcgaaccc aaagtcgtat
     9661 tagccatcta ttcttatttg atatcacaac atatcccaaa cttttgggta ttagtgattg
     9721 cgtggtgaat attgccccag accttgccca gaagaaacat attctgcgca atagcctaga
     9781 agctttagag cgactcgata ttaaacatgc aaaagttgcc attgttgctg ctacagaagt
     9841 tgttaatcca gccatgccag caactacgga tgcacatgca ttggtagaag agcatcgaca
     9901 cgatgcgatt tatccaggcg ccctaatcga gggaccattt ggttttgata atgctatctc
     9961 tgcagtctct gcgaagacca aagggattac gtcttcggtg tcgggtgacc ccgacttaat
    10021 tctggtacct gatcttcagg tgggaaatat tctgtataag gcactggttt atttgggagg
    10081 tgccgagtgc gctggcgtga ttttgggtgc cagcgtccca atcatcttga cctcgcgctc
    10141 agactctatc ttctcgcgtc ttgcctcgac tgcgcttgct ctgcacttag ctaaaaatta
    10201 gggaataatc tcctctacct tcatttcatc ttaaccatgt tcaaaatctt ctcgtctgac
    10261 cctgtccacg atcccattac caaggttgga cctgctacgg aaataaaaac caccacctgt
    10321 tatatgtgcg catgtcgttg cggtattcgt gcgcatctgc gtgatggcga gttggtttac
    10381 atcgatggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncagaaa
    10441 tcccctgccc ggatcacgaa gccactctta cgcaaagcag gtgctgatcg aggcgcatcg
    10501 gagttcgagg agattagctg ggaagaggcc tttgatctcc tggccactcg tttagcgaag
    10561 atccgcgaga ctgatcccaa gaaatttgct cttttcacag gtcgcgatca aatgcaggcc
    10621 ctcactggtc tctttgcacg ccagtttggt acgcccaact atgcagccca tggcggcttt
    10681 tgctcggtca atatggctgc cggcatgatc tacaccatcg gcggtagctt ttgggagttt
    10741 ggtggacccg atctagagca agctaaatta tttgtgatga ttggtacagc tgaagatcat
    10801 cacagcaatc ccatgaagat tgctttatcc aagttcaagc gtaatggtgg gcgtttcatt
    10861 tctatcaatc ctgtgcgcac aggctattca gctattgccg atgagtggat acccattaag
    10921 cccggtaccg atggcgccct ctttatggcc ttgatgcacg agttaattgc tgctaatacc
    10981 gttgatcatg agttcttgtc acgctatacc aactctgcgc aattagtttg tcttgagcct
    11041 ggtcctgaag aaggactctt cttgtttgat cccgatagcg atccaatcaa taccgatatt
    11101 ccgcacaata aatatgtgtg ggatcaaaac acacaaagcg ctaagccttg ttttgatcag
    11161 actgcaaagc cagcattaag tggcgagttt gtgatgggcc ctggtcctca tcagggcaaa
    11221 cgagttgccc ctgcttttga gttactgcgt cgccaggtta aggcatgtac accggagtgg
    11281 gcagcgaaga ttacatccat accagcagag cgtattcgtt tacttgcaaa agagatggcg
    11341 cataccgcat ttcagcaagc ctttgagtta cccatcgcat ggaccgattc gtttggacaa
    11401 aaacacaata ctgtcaccgg tcgacccgtt gcctttcatg caatgcgcgg cctgtctgca
    11461 cactccaatg gttttcagac cacacgcggt ttagcagtct tgatgacctt gttgggcacc
    11521 atcgatcgtc ctggcggctt tcgtcataaa gctccctacc caagacagat accgcccaat
    11581 atcaaaccac cctcttcgga ggcagacatt aagcccaata ctccgctggg taaagcgccc
    11641 cttggttggc caactcgccc agaggatctt gcgctcgata gcaatgagca gccactgcgc
    11701 atcgataaag cctatagctg ggagcatccc ttagcagcgc acggtttgat gcataacgta
    11761 atcaccaatg cggttaaggg tgatccttac tcgatcgata cgctaatgat cttcatggcg
    11821 aatatgtcgt ggaactctac gatgaacacc atggaggtgc gcgagcatct caacgccaaa
    11881 gatgagaatg gcgagttcaa gataccgttt ctggtgatct gcgatgcatt ccaatcggag
    11941 atggtagctt ttgccgatct ggtcttgcct gatactactt atctggaacg acatgatgca
    12001 atgagtatgc tcgatcgccc gatctctgag tttgatggac cttgcgactc cgtgcgcatt
    12061 ccagtattac caccgactgg ccaatgcaaa ccgtttcaag aggtgttgat tgagttggca
    12121 tcgcgcctta agttcccagc atttacaact gcagaaggcc agcgcaagtt taaggactat
    12181 cctgacttca ttactaattt ccagaccgca cccgactccg gaattggttt cttaatgggt
    12241 tggcgcggta aagaaggtga aaagagtatt cggggggagc ctaatcccaa tcagtggcag
    12301 atgtatgaag acaataactg tgtctttcat tacagcatgc cacccgagca tcactacatg
    12361 cgcaattgga atcaaggcta tcttgatttt gctaaggcta acgctttacg tcagaagaac
    12421 gatccgatta tcttggcggt gtactcagac atcttgcaac agtttcgttt agcagctaag
    12481 ggccagcgtc cgggcagaac accacccgcg catctcaaag atcgcattgt gcaatttttt
    12541 gatccgctcc ccttttggta tccacccctt gaggatgcgg tcacggatct caatgagttt
    12601 tcgattaacg cgattacaca aagaccgatg gcgatgtatc actcgtggga ctcacaaaac
    12661 gcatggttac gacagattca tagtcataac tatttgcaca tcaatactaa gaccgcagtg
    12721 gatcatggta ttgaagatgg tgcttggatt tgggtcgagt cgcaatgggg taaggtgcgt
    12781 tgcatggctc gccatactga ggcagttgac cctggtaccg tctggacctg gaatgcgatt
    12841 ggtaaagcag agtctgcctg gcatcttgat ccgaacgctg atgagtctaa aaaaggattc
    12901 ttactcaatc accttattac cgaggagatt cctctggctg atagcgctgg cacatttcgt
    12961 gtgagcaatt cggatcctat taccgggcaa gcgggttggt atgacgtnna ctgccttagt
    13021 acctggaatg attcatggaa aataatatgc cagcctctac tcaatctagc aaacaacttg
    13081 ctctagtcat tgatctcaat gtctgcgttg gctgccatgc ttgtgtaacc tcctgcaaag
    13141 agtggaacac gcaaggctct gccggtccca tgaccgatca agactcttac ggtgcaaatc
    13201 caagtggtac tttctttaat cgtgtgcaaa cttttgaagc aggcgttttt cccaagatgg
    13261 atacggtgca cttccctaaa tcctgcttgc attgtgaaga tccgccctgt gttccggtat
    13321 gcccaacagg tgcgagctat aaacgcaaag aagatggcat agttttggtc gattacgaca
    13381 agtgtattgg ttgtaaatac tgtgcttggg cttgtcccta tggcgcacgt gaacttgatg
    13441 aagaacgtca ggtcatgacc aagtgcactt tatgtgttga tcgtatttat agtgagaccc
    13501 ttcccgagtc agagcgtaag cctgcttgcg ttttagcctg ccctaccagt gcacgtatct
    13561 ttggtgatgt gcatgatccg ctctccgatg cgagcaaagc cattgcagag cgcggtggct
    13621 atgaactcat gccagagtgg gagaccaaac cagccaatca ttatttaccg cgctccatta
    13681 ctgagaccat tgcggccgtg aaagcagaaa actaatgcat ccgaccttct ccttaattct
    13741 ttttacaagt atggcgggga tggctcaggg ggctattgtt agtcttgctg tgctcaactc
    13801 aggttctgag caattaccaa gtgagctctt aaatatttat ttattcccct tgatcttggc
    13861 tttactaatt ggcggattac ttgcttccac ctttcatttg gggcatcctg aacgtgcctg
    13921 gcgtgccatc atgatgtggc gtacctcatg gttatcgcgt gaggttttag tgctcccagc
    13981 atttatcgca ctcacggcat tagcctatta cttctcatgg caagaccggg ttcccaattg
    14041 gttatggctt gttttgtgta ttgcctcact tgctctttgg gtttgtaccg cgatgatcta
    14101 tcagtgcatt cgttttatac aggaatgggc gcatccaacc accatggtca acttcatcgc
    14161 attaggaatt agttccggtt ggttcttcct catggccttg ctatcgcttt ggtctatgct
    14221 gcaccgtgat caagccgtgg tcaccagttc taatatcgct ggggttgctg gctttacggg
    14281 gttcttaatt cttttatcgc ttacgctgaa gctgtggatc tggaaacgta atcgcagtct
    14341 taagcctaaa tctaatttac agtcggctac tggtattaag accggctttg tgcgccagat
    14401 ctcgatgggg atgatgggag gtagttttaa tacccgtgag tttttccatc aacagagtgc
    14461 cttctttgta gccaatgtcc gtaagattgc tttctttgga acctatttac tgccagttat
    14521 tttgctggca attgtggtga gcaatggcgc tcttggctta ctggtgatcg catttatcgt
    14581 tcattgcgct ggattgatgg ctgagcgttg gctctttttt gcagaagcca atcacccaca
    14641 aaacttgtac taccaacgaa ttagttaaat gaattgcttg tttaaatacc gagttcgctg
    14701 aagtactcgt ggtgttcggt atttaatgtt gagatgcgcc attcgatggg ctcatcttgg
    14761 atgcctagtg caagacgccg aataataatc accggtgcgc ctactttgag atccaaccat
    14821 tctgcatgtt gtttgctagc tgctcccgca cgaagtcgtt cgcttgtgcg cacaacagtt
    14881 tgtccatact gcgcttgata gagctgataa atgctgcccc cgcgttgtcc aaaggccgct
    14941 ttagtcagct tggggaagtg tttcttgctc agagtaatgt gatcgaccaa tacacaggtc
    15001 tgatctaaaa agagacgatt gacgattctc caaacaggcg aaccttcttt tattgcgagt
    15061 ttagtagctt cctcttggtt agcacttgcg ctctgaaagg agacaagctc taccttgggg
    15121 tagtttttat gttcagcatt gtgtttgact acatgaaaga agtaatacaa aagtcgttgt
    15181 tcatcgtgct cagcaacata ggtgcccttg ccttgcctcc gaatcacaat tccttcagcg
    15241 accagttcgt cgactgcttt gcgaagagtg ccaatagaga ccttgagttc cttagcaagg
    15301 tctttctcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
    15421 gttgggtgac caagcaggct acttaggcag actcatataa acgggtcatt acaaactcgt
    15481 tatgaccaag gatctctgcc gcggttagtt cgccattggc ggtgcgcaag agcgcatcca
    15541 atagtttctc acccgctgta tccatgttct cctcacgacg caagatccct gagacatcca
    15601 catcaatatg ctcagacatc gtgcgaacag tcttgggatt agcacaaagc ttaatcacag
    15661 gcaggatggg attgccaatc acgttacctt ggccggttgg gaagaaatga gcagcaaagc
    15721 ctgaggcggc gcacaaggtc accatctcgg cagcagccga tgaagagtcc ataaaccaaa
    15781 gacccttacc tgttggtgct tctgctttat ccaaaacacc gtcgaccata cacttcttac
    15841 caatcttctg aatattgccc aaagctttct cttcgatggt tgtaaggccg ccagcaatat
    15901 tccccttggt aggctgtgag tctgataggt cgctagtctt attgcgctcg accacacctt
    15961 gatagcgatc aaacatcatc ttgaacttgg ctttgacttc tggtgtacgg caacgtgctt
    16021 ctaccaaatg ctcgccacct gtgagctcgg tggtctcacc aaatactaag gtggaaccaa
    16081 tttcatagag tttgtcaaaa gcgttaccaa cggttgggtt cgaaccgcag cccgatgtcg
    16141 tatccgactc accgcacttg gttgaaaccc acagctccga taaaggtgca gagacgcgtt
    16201 gtgctttgga ggcatgcttc atgaacttat aggcagcacg acttgcatta gcaatcgtgg
    16261 tggtatcgcc attaccctcg atccagaatc cttctactgg cttaccggat tttttgattc
    16321 cttctacaac cttagcggtc cattggggct caataccaat cacgactaca gcagcaacgt
    16381 taggatt
//
LOCUS       DDPQ01000081           18635 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_9704,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000081 DDPQ01000000
VERSION     DDPQ01000081.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 18635)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18635)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18635
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_9704"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    828..868
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4410..4478
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10924..10993
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11336..11393
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13243..13261
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14469..14540
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4517 a         4231 c         4346 g         5210 t
ORIGIN      
        1 cgctccttcg ctctttgggt atgtttaaag attgcggcgc tcactgcaaa ccatgcttgg
       61 ttcagaacaa ttgcgtcgcg ttgtgttcgc cattcaatgt attgctcaca cactcgtccc
      121 caatattgcc gaacttgaat gtactcttgg gcgttgtgct gttgcgcttc gagataggcc
      181 aagatggtct tactagcgct gtcattatcc ttgcgataac tgggtaactc attttgggtc
      241 atgaacactg gaacaattgt ggtggccgcg ctggcaaggt cattgtttgc gagatgctcg
      301 atcgtgagct caagatcagg aactaaattt tggtccttct tgccgccgtt ttgtaaacca
      361 tcgagtatct gttttagttc ttctagtgct tgctggttac ctaaggcatc aaggagtggg
      421 tttgggcttt gatggtcttt gcaataggct ttgagaacct ctacaggggt cagtccttgc
      481 gccttgcaag ctttctcaaa gaagacccaa gcgcccttct gtcgcagcaa gctactcgac
      541 cccaacagaa attgattagt ttgataagaa cctaggtgat cgagtaagac ctcatagtgc
      601 gcttcaatct ctttgggtaa tgatgcccac caatcctcta gacattcggt ttgtaatcgt
      661 tttgcatcct cgcgtagact aaatccttgc tgtgctccca tcgataccgg tgcaccactt
      721 aagagagtac caaaccaacc atgaaaggta tcgatgacga ccgaatgcgg atttgccaac
      781 aaggtctcat ataaagcgcg cgccggatgg atcgattgca ttgcctcnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cgactctcct gctaacagaa tccgaattaa
      901 gcgcgccact agcaaccagg ttttaccgct ccctgcacac gcagagacta cgaccgagcg
      961 agcgggattg cagactagtg ggatattgag tttgctaggc atgggtatca ccacattcct
     1021 tttctgcaaa taccacgggc ctcgcaatag cggcaggcac tctcgggtgc aaaggcttgc
     1081 atagccgctc ctgaccaaac cgcactgagg tcttgttgca actgtttctc aaggaccaag
     1141 acctgatccg ataaatgatc aagcttgaac gagcgctcgc tggaatcttt tttcttgaga
     1201 ttcattttga gagcgaccca atcggcctcg atgaccggat ggtctgtggc cttttgctcc
     1261 tcggtaatag ccttggtgta gagcaagagt tgcgggtcat ccatcagttg gtctgctcgt
     1321 tttttgatct tatcggggct ctgatgtttg taatcgagca ccgccgcttg cctgagagtt
     1381 gtattgatgt ctaatcgatc ggcatatcct tcaatgcgga cagtatgttc ttggtggtga
     1441 gcatcagtga atgaaaactc aaagccgacc ttgagctcag catcatgaaa ttgccagcca
     1501 tgactctcgc gctctaattg ccacgcgaca aagcttggga tttgtttttg ccaatcccgc
     1561 aagcttccta aaattcgttt atcaccctca agtagtttta gaaagccctg ctctgaagca
     1621 tggtgcaaat gctcttgcat ccaggtttgt cggtattgcg gatcctgtaa acggggctca
     1681 cggatctgag tgctcttaag ttcttgaaag aagttgcgca agatctgatg taaggtctga
     1741 cccagcaggg atgcatcgag ctcttcgtca agtcctttgc gcttacgcaa acctaataag
     1801 ttgcgcacat agtattgata gggacaggat cgaagtgctc ggtaggcact ggggctcatg
     1861 cgggttggca tcggaagacc gggtagtcga tgtgcgctag ccatagcgct tggtcttgct
     1921 tgtgcggtgc gttgctctgc ctttgcctga atggtcgcta atgttggtaa gtgattttgc
     1981 agtctctgta tccaaggtga tgctcgcaat ggctctcctc gagctccctc actctgccaa
     2041 agaagatcga tgtgctcata actggcgatt agctgtgaga gatctcttgc ttgttgacgg
     2101 aattgccaag cgatactcga ccctcccaat acttggcaga gcgtctccga gaaaaataag
     2161 ggtggctctg aaaacgcggg taactggcgc tcatcgcaac ccaccatgac cacggcatca
     2221 aaatagcgca agcgggtgga gctcagtggc aagatcgata cgcgcgcact ggttcgtgca
     2281 ccctgctctc gatagacctc ttcctccatg acggtcttta gcaagatcaa ccactcgttt
     2341 agaggcatgc gcaactggcc taacttgctt tgttgcattc caagtgtttg cagcatatgg
     2401 agcaactgca ggcccgcagc atcttgctca aagccttgct tcattccaaa ggctgatagg
     2461 tcttcgtgaa gttgtaagag agcttctgcg caatacggtg tttgcattgc tttgctctgc
     2521 cattgcccca cgcgttgccg aatcacttgc aatagggtct gtaagcgcgg atcgtattct
     2581 gagctagctc ctgcctctat tgctagatag aaactgaccc aggaggagcg tgcctgttta
     2641 atcagtaagg cgttctctaa ctcggtgatg agcagatcac aattactctc ttgggtatta
     2701 agaagatgct cgagatccag aaagggattt tttaagaact ccagtaatgt actagcgctt
     2761 ggtccttcac tgggagatcg caataactca agccatgcat gcagtgccgc agcagcgcgt
     2821 gtcgtagaca gtttccagcc ggtctcatcg gcaatactta aactaggacc taggcgcgcc
     2881 aatagtgcac gggttcttct ggctaacaat cgatcttgtg cgactaaagc aatgtgtttg
     2941 cgcccctctt ttagatgcgt ctcaatccgt cgtgttgcct cccaggccag atcttcaaag
     3001 cgatttgcag taatgcactg aatggttttg gttttgaggg tatgaatatt gtgctgcgcc
     3061 tgcgcctctt cttgtgcacc aacttccgcg ctgatggcct cagaccacaa ggcgactgag
     3121 ctccaatcca tttgcaagcg atgcactggt atgtaaggtg tgcaggcttc caagaattgg
     3181 gtttgcatac tagcttgcgc aggttgcggc tcgacggtat caatccagat taggggtctg
     3241 cgcgactgtg ggtgttgtgc aaactgctga acataagagg ctaaggccaa atgcttccga
     3301 atgatgggat cgcggaccga gcctagatat ctccagaatg ccaataggac atttgcttct
     3361 ttgctaacca aggcgtgcgc tagttttggg tatgctgtgt cgattgcact ttgcaagaca
     3421 gactcaagct tttgcatatc gcccttgaga tcgagcgtat tcggatccca cgataaatgc
     3481 ggcatgatgc tttgtgagag taactcacag gcgctcacaa tggcttgcgc aagactccac
     3541 accccgccct ctccttccgc agcaaaccag gtttgtaact cagggtgagc atcgatcgat
     3601 gcataggttt gtaagatgcg ctcggtttgc gattgttgat cgggtatcaa gaaaatatct
     3661 ggcgctagct ctaaccaatc actaagactg tgcacctctg gcaaaaaggg aatatttgct
     3721 tgatccaagg aaggccgaag gcgctcaagg gcttggcgca aactggcatt cggtccagca
     3781 gtggggagga tcacgagcgg tctagtcttg ctgctcttag caatctccca aatatgctcc
     3841 gccatctcca aaagggcatt ttgattgggg gtgatcgtga gggtttgcat agcgagtgct
     3901 tattttgcaa tcgttctaag ggaattgggc atggttcaca gcttattgat aatataaggg
     3961 cttgggacgc tttccaatga gagcagttcc aaaccgataa attgataagg aatattcatg
     4021 agtgccggtg ttaaacatgt aagtgatgca tcttttgaac aagacgttct aaaagcagat
     4081 aaacccgttc ttctcgactt ttgggctgag tggtgcggcc cctgcaagat gattggtccc
     4141 atattggagg aactttcggc tgagtacggc gatcgcatcc agattgccaa gatgaatgtg
     4201 gatgagaacc aaggcattcc tgcccagttc aatattcggg gtattccaac acttattttg
     4261 ttcaaaaatg gtaccgttgc agcccagaaa gtgggtgcat tagccaaatc ccagctgtca
     4321 gcgttcatcg atagcaatat ttgaggcact tcttagtcat tacaatttac ccgctagaat
     4381 gaccgatatc aggctcagga gttgagcctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg cagagtccgc ttgaggctct
     4501 ccccaaaatt tctagtttct ctcccaatct acctttaata ccctgattac tcatttcgat
     4561 ttaagctaaa ttcgttatca ctgttttgca ttgccgtctt gtatacccga gcggcgatcc
     4621 caaaacccct ctaatttctc ccccttcctc ttatttccct tcctttttcc tgtttgatag
     4681 aaccataagc acaccatgca actgactgaa ctaaaagtac tccacgtttc ccaacttctt
     4741 gagatggctg ctagccttga gatcgagaac acccagcgca tgcgcaagca agagctgatg
     4801 tttgcgatcc ttaaaaagcg agccaaggcg ggtgaacagg tatttgggga tggggtctta
     4861 gaggttctgc ccgatggctt cggtttctta cgctctcctg aatcctcgta catggcctcg
     4921 cctgatgata tttatatctc ccctgctcaa atccgtcgct ttaatttaca tacgggtgat
     4981 ggcattgagg gtgaggtgcg cactcccaaa gaaggtgagc gttactttgc cttggtgaag
     5041 gtagacaaga ttaatggcat ggccccagaa gatctcaaaa accgtatcat gtttgagaac
     5101 ttaacaccgc tccaccccaa tcgcactatc tctctagagc gcgatatcaa ggcagaagag
     5161 aatctgactg gtcgcattat cgatatgatc tcgccgattg gttttggtca gcgtggcttg
     5221 attgtttctt ctcctaaatc aggtaagacc gtgatgatgc agcatgtggc gcatgcgatc
     5281 tcccagaatc atccagaagc cattttgatt gttttgctgg ttgatgagcg tcctgaggaa
     5341 gttactgaga tgcaacgctc ggtgcatggt gaagtcgttg catctacctt tgatgaacct
     5401 gccgtgcgtc acgtacaagt tgcagagatg gtgattgaga aagccaaacg tttggttgag
     5461 atgaaaaaag atgtcattat tttgctcgac tcgattacac gcttagcacg tgcttacaac
     5521 acggtagtgc cttcatctgg caaagtactc tcaggtggtg tcgatgccaa cgctttgcaa
     5581 cgtccaaagc gtttctttgg tgcggctcgt aatgttgaag aaggtggctc cctcactatt
     5641 ttggcaaccg ccttaattga gaccggaagc cgaatggacg acctgatcta tgaagagttc
     5701 aaaggcaccg gcaatatgga agtgcattta gagcgtcgcc tagctgagcg ccgtgtctat
     5761 cctgccatca acctcaataa gtcgggtacc cgccgtgaag aactcctggt taaacccgag
     5821 aatctccaaa aaatttgggt gcttcgtaag ctcctggctg atatggacga gatcgaggcc
     5881 atgaacttta ttgtggataa gctcaaatca accaagaata atgctgagtt ctttgacctg
     5941 atgcgccgtg gcggctaagg cctaggaagg cattttcgat gctttcctat tgattttgtg
     6001 gcataatttc gcttttgctg tattgaccac ctgggcaagt attgagggaa tccctttttt
     6061 gaccttcaac ggctacccgt accaaggata ttgattatga aaccaggcat tcaccccgaa
     6121 tatcgcgaag ttgtttttgt tgatgtttca aacaacttta gcttcaaaac tcgctctacc
     6181 atcaacacca aagagaaaat caaatgggaa gatggcaatg agtatccatt agctaagatt
     6241 gagacctctt ccgagtcaca ccccttcttt accggtaccc agaagattat cgacaccgca
     6301 ggacgtgttg agaaattccg ccagaaattt ggtggtaagg ctgctgcgaa gtccactggt
     6361 gacggcgctg ttaagactgc tgagaaacgc gctgcagctg ctgaagccaa gaagaccgag
     6421 aaacctgctg ccaagaaagt ggctaccaag aaagcgaagt aattgcttcg gcatggtttt
     6481 aatcttgact tgatgtacac aaggcagcgt ttgctgcctt ttttatttct gttttattct
     6541 ttttagtatg gtcaaactca ctgctgccgc caccgtctca attccacgca ttgtgatctt
     6601 tgcgttgacc ctcgtttatg gtttagcggg tctctttggt cgtgatcctt ggaagaatga
     6661 agactccatt ggttttgggg tgatgtggaa tttgcattta ggttcttggc aagactggtt
     6721 ggttccgggg cttgctggtc gtgatctttc catgggtgca ccactgccct actggttagg
     6781 tgcaacattc attgatctat ttggttcaat aatcggtgac gccaatgcag ccagactgta
     6841 ttcagcgctt tgcttcttta cttctgccat tgcgatttgg tatgccacgt atttactagg
     6901 acgtcgtcag gaagtgcagc ccatgagttt tgccttaggc ggtcaaccta atacacgcga
     6961 ctatggcatg accttagcgg atggggcatt gctcatcttc ttagcctgca ttggtttggc
     7021 acagcgttct catgagacca cccccatgat ggctcaccta atggggctct cgattatttt
     7081 gtatggcact gtnnggctta gttattctgg ggctctctag taattgggcg ctgactgctt
     7141 taattgccct ggccatcggt cttgcagttt tgctgtgtca ggtaaaactt cgttttcggt
     7201 ggacccttag tgctattgcg attgcaattc ttggtattgg tgtttggccc ttactgtggc
     7261 acgtgtttca tcttagccca gagttgcaag attcagccct aaacgcgtgg gctaacacgc
     7321 cgcccatgca tcgctatctt gcttggggct ccttgcagtt tatgagtatt aatttttggg
     7381 cttacgcctg gcctgtttgg cctttagcct tggtgtcttt ggttcactgg ggtcgcaacc
     7441 acgatgcagg cacatggcgt gctccacata tctgtattcc gcttggttta ttgctggctg
     7501 gttttattta tgtgctattt cgcgttgagg ctaatgaaca tgatctgatt gtgttgatac
     7561 cgccgatggc tattttggct gcatttagtt tgcccatctt gcgtcgtagt gtcattagct
     7621 ttattgattg gtttgcaatg ttgagtttta cgatcattgc tattgcgatt tggctcattt
     7681 ggtttgcaaa gaccactggc tatcctgcac ccattgctgc taatgtggcg cgcttcattc
     7741 ctggctttga agctcaattt agcgggctaa ctcttgtgat tgccctcatc attacagcac
     7801 tttggttatg ggtagtgcaa tggcgtacct cacgcgctcc aaaggttatt tggcgctgct
     7861 tggttatctc agcagcaggc acaactctga tgtgggttct tttgatgacc ttatggcttc
     7921 ctacgattaa ctatgccaag acctatcgtt ttgtggctga gcgtttggtc aaggcggtac
     7981 cggcagatgc taagtgcatt gataccagtt atttaggcct agcccagctg gcatccttta
     8041 gttacttcac aaaactagcc ttggccgaca accccaattg cccctatatg ctgactcata
     8101 atgccagcac cgcctctgcg ttctcggccc tcaacgataa gaaactcaag ctattgtggg
     8161 aggatcgtcg ggcatctgac cgcacggagc gtttgcgttt atatcaggtc ttgtctgagt
     8221 aactgatcac gatgtccttt tatcgaatct cgcgattgcg agaagatatt cctgagcttc
     8281 tcaaattagc cggccctctt ttggtaggcc aactagccgt cattacattt ggggtcttag
     8341 atacggcaat gaccgcacgc tattcctcag aagatcttgc ggcattagcg atggcaagtg
     8401 caatctttat tagtgtttat gtaggtttga ccggtgtaat tgccgccctc acccccattg
     8461 cgggtcaact ctttggggca aagcgttatg cggagattgg tgaagaggtt cgtcaggctg
     8521 gctggctggc ggtcggcttg acgatcgttg gcaccctgat tcttttgaac gctgaccact
     8581 tgctagcgat ctcacaggtc aatcttgatt tagagaacaa agcccgctta tatcttcaga
     8641 tccttgcctt aggcctacct gccagcatgg gcatgcgggt tttaatgtct tttcataatg
     8701 ctgtttccag accaggcatt attactatcc ttcagttgat tggtttagcc gctaagtttc
     8761 cgcttaatgc gctcttcatc tatggcggct ttggcattga tccaatggga ggccccggtt
     8821 gcgccgttgc caccgtcatc attaattggt tgtggttttt ctcaatcttg atcattgtgc
     8881 tgtggggtcg gttttataag ccctttaata tttttgagcg ctttagctgg cctaactggc
     8941 atcgtatctg gacgctatta cgcttaggcg cacccatcgg tttaagttat ttcattgagg
     9001 tcacctcgtt tacctttatg tccctattta ttgctcgtct gggaactact acccttgcag
     9061 ggcatcagat cgtggctaat ttaggtacgg taatctatat ggtgccactc tcattagcaa
     9121 ttgcgaccat gactctcgtt tcccaatcga ttggggctgg caaacaacat cgcgcagaag
     9181 aaattggttg gtcgtccgtt ctattcactg gcggcttatg tattgtgatt ggtctattag
     9241 tttgggtgct gcgatttcag ttgcttgatc tgtacgaccc aacacccgat gtcaaactat
     9301 tagcagcgcc tctattctta tttatttgct tttatcaatt ggtagactca gtacaagttg
     9361 tagctgcatt tattctgcgg gcctatcgca tctctttctt gccaatgatt gtgtatgccg
     9421 gctctctctg gtgtatcggt ctaggcggcg gctatctctt gggctttaat gtgtttggta
     9481 atacgccaac actattacaa ggagtcaatg gattttggat tgcaaatagt ttgagtctag
     9541 ctgtagctgc tgccctatta ctctggattt ttagaagaac ggcagcccac tatgaaagaa
     9601 ctaagccgcc ggtgacggtc tagctcttct tatcttgaat gagtaacatg ccaagctctt
     9661 tgtacagctt cattgctttc tcagtgggta ttaacttttt taccctagcg tctttggcac
     9721 acggcctgtt ctcaatcaat tttttctcga tcagggcctt caaagaagca tgcgaagttg
     9781 caggtgaggc aatcgatttc atctgaatca catcagacac tgttaattta tgaccctcat
     9841 aatgggcgct agctatcatg cgaagaatga tgcactcacg atgactgacc ttgtacttaa
     9901 gatgaatctt gtcacgactt tcagcaaaac taaagtaatt agacagtagg tccatagcag
     9961 tccctttaat agtaattatg gggaaatata agttggatta ccaaaatagt aaatcaaatt
    10021 ttacacctga ttagcccttg ggtgtatgaa cgatcctttt cttaccaaat ccccccccct
    10081 cttcaattat tagcaaagac ctaagctttg caggctattt atttgtgtat ccatggaaaa
    10141 gaatataatt ttcataaact tatacaaatt aaatagaaat gtggatttat gctggagcga
    10201 ttttttaaga aaactagtcc atttgtcatt aaatctattg actcaaccaa aaaagtagcc
    10261 gtagttgagg ataaagaatt aggcctcgtt attgaggtgc cctacggtcc tagcatattg
    10321 aaaaaggctg aaatcatcag cgaatatgaa atccagtttc tctacgaaga tggaagctca
    10381 agaatctcta aaatcctaat gtagtgcttg cagtaagacc actcacttaa tcggcttgaa
    10441 ctcattgacc ggtttaaaac caggaacctg attaccttta gcgtacatac attgctgata
    10501 agcagtgttg tattgagttt gtgcttgagt ctccttattg gatgcaccca ttgctccaac
    10561 agcagcgccg cctaccattc caatcgccgc tcctgtgcca acgttcggac cacttcctcc
    10621 ttgatacatc gcccccgctg ctgcgccaat ggctgcccca gttgccgcac taatggctcc
    10681 agccttgaga gcagctgcat tgccatcttg aacagaattt tgagcaaact ctcgacactc
    10741 ttgatcttct tttttaaata cttcaaaggg ctttccttct ctaggcataa cagtgatggt
    10801 cggccctgtt ggggcagaga cgcaagcggt cagaaccaga ttggataagg taacgccgac
    10861 taaaagaatg gagtgtagtt tcacgagcaa cctaaattag tgattagtag atggatgata
    10921 aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnaatcggg tagtactgct gttgtggttc gcagtaatac cagaccggcg
    11041 gttgtgactg tgagatcaag ggaagcactg cgggtgctac gtaatagggt gcggggtaat
    11101 aaacaggtgg tggtgaataa taagttcggt agtatggatt gtagtgacgt ccataaaagg
    11161 gtcgagggta ataaggcccc acatatggcc tgccgtaata gccaacccca gttcctactg
    11221 aaatattcca tgaatactgc ggtgctgctt gtgccaatga actggctgtg atgatgagca
    11281 aaaaaggcaa atacctgatt aatctcatct ttatctccta tttgggtgat agcaannnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttattc
    11401 cgcccttgca ccagactgct gaacaatctt gcgccattta gaggcttctt gctgaatata
    11461 gcgactgagc tcttcagggc tgcctgggtt aggatctaac cccccgtttg agagacgaga
    11521 ccgaatctct ggttttgata aggtctgaac ggttaattga ttgaggcgag cttgtatctc
    11581 tttaggcaat ttagctgggg ccatcaagga gaaccacgat accgcctcaa accctcttaa
    11641 tccctgctct gccaaagtcg gaatatctgg ggctgatggc gagcgcttga gggttgttac
    11701 acctaaggcg atgacttcat tggctctaat taatggttgt gctgatgcca agttatcaaa
    11761 gagcatggag atgcgtccgc ttacaagatc ggggagagat tgtgctctgc ctttgtatgg
    11821 aatatgacgg atctgaacac ctgccagatc tttaaagagt tccccggaca tatggattga
    11881 agttcccgta ccagaggagc caaaggttag ctcattgggt tttgacttgg ccagagtaat
    11941 aagctctttg agcgattgaa cacctagctg tttgttaaca atcagcacat tgggggtgct
    12001 tgctagaaaa ctaatgggcg tgaaatccgc aatggggtca tagggtaact tctcgtaaag
    12061 agcgccatta atcgcatgaa tacctacggt tccaatcaat aaggtatagc catctggatt
    12121 acttttagca actagatctg cgccaatatt gccgccatga ccagggcggt tatcaaccaa
    12181 aatgggaaca cctaaggtct tttgccaatc ctccgccaag actcgcgcca aggtatcagg
    12241 cgcaccaccc ggggtaaagc tcacgacaat ccggatggtc tctctaggcc atgatgaatt
    12301 agtctgagca tgaacagcaa aactaccaag tagtatgctg agccctatga gagctctttg
    12361 tattaaaccg cttcgatttc gacttaaatc actagtcatt gcaaacaatt aaaggagggg
    12421 cacattgttg caaatgataa gcaaattctc atggggtgaa ttttacaaga gcaatgtgta
    12481 ttaaccaaaa gggttttatg tttagaatgc tgtgatgaat tcttctacag aaagcactaa
    12541 acgccccctt cctctggctg gtgtcaaagt tcttgatgtg agtcaagtga tggccggtcc
    12601 ctactcctgc atgttactgg cggatatggg tgccgatgtt attaaggtcg agccaccggg
    12661 tgggggtgat caaacacgcg gtgcgatggg attcaagatg aagggtcccg atagcatggg
    12721 atttctcaat atgaatcgta ataaacgaag cattgcgatc aatctcaagt cggaagcagg
    12781 caaatccgtt ctgtttaagc tggttaaaga agccgatatc ttggtcgaga actatcgtcc
    12841 tggagttatg aaacgcttag gtgttggcta tgaggtcatg agcaagatta atcctgcgct
    12901 cgtttatgtc agcatctcgg gctttggtca gagcggtcct tgggctgagc gcccaggctt
    12961 tgatttaatg gctcaggcga tgtcagggat catgagtgtc accggccatg gtgatggtaa
    13021 gcccgtgaaa gcgggtgtgc cggttgctga tattggttgt gcgctctttg caacgtatgc
    13081 cgctctctcc gcatatattg gggcgaagaa taccggtcaa ggacaatata tcgatgcctc
    13141 tttatttgat tctgctttgg ctttctccat ctgggatacc tccgaatact ggggcaccgg
    13201 tcaaccacca gttgcactag gcaccgctaa tcgcatgacc gcnnnnnnnn nnnnnnnnnn
    13261 ngatggttat tttgtgatgg gtgcaaccaa taacaaactc tggcagaaac tctgtgagat
    13321 tttgcaacgg cctgacctga tccaagatcc ccaataccaa accattgcgg gtcgcctagg
    13381 tgagcgcgag caattaattc atgagcttga gcgctctttt gccttaaaaa ctgcggatga
    13441 gtggattgat ttactcttgg cgcaaggcat accagcgggt cctatcctag attatccgca
    13501 agcatttgac agcgagcatg gtaagcatcg ccaaatgcgt atcgagatcg atcacccgct
    13561 tgaaggtaag gtccccaata ttggttttgc ggtcaagatg caaggtactc cgcaagaagt
    13621 tcggcgccac cctcctcttc tgggtgagca tacccaagag gtcctaatgc aagcaggatt
    13681 tacagatgat gagattaaat tattagaaca acaaggagcc tttgccgcat gaatgccaat
    13741 gctcgcgtct atgtggagat caaagggaag tttgcgcatc tattttttga tcagcccgcc
    13801 gcacgcaatg caatgaccca agagatgtat gagcaattgc gcagtatttg tctggagctt
    13861 aatcagaact ctcaaatacg tgcagttatt ttgcgaggta ttggcggtaa atcctttgtt
    13921 tctggaagtg atattgcaca gttcactgct tttaaggatg gtgaggatgg agttcggtat
    13981 gagaaactca ttgatcacta cctaggacct ctccagcaat taagcatgcc ctccattgcg
    14041 gtcattgatg gtttagctgt cggtggcggc ttagcaattg catctctgtg cgacttccga
    14101 attgcaatgc ctaccgctaa gttcggagtt cccatcgccc gtactcttgg taacacttta
    14161 tcgccgagta atattgcttg gttagtggcc caccttggtg ttgctattgt taaacggatg
    14221 ttgctcctag cagagttaat ccccgcatcc gagctacata ctcaaggctt tgtctacaaa
    14281 attggtgagc aagagcaact tgagcaaatg gcccttgact tagcagaaca gctctcagcc
    14341 ttggctcccg tgactcagaa ggcatcgaag cagatcatgg cacgggtaat ccatcactct
    14401 ttacctgatt gcagcgattt aatccgcgag gtctatggca gctctgactt taaagatgga
    14461 gtacgttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn aatgtctttg gacaacctct ggttccctgc tcgtttgatc
    14581 ctttaacagg attttttcgg gatggttgtt gtaaaactaa tcaagaggat attggtttgc
    14641 acttggtctg tgccatcatg agcgaagaat ttttagagtt tagttaccaa cggggtaatg
    14701 atctcattac acctcgtcct gagtttcagt ttccgggtct aagagctggt gatcaatggt
    14761 gcctatgtat cacccgttgg caagaagcac ttgccaacgg ctgtgcgcct cttataaaac
    14821 ttgagagcac tcatctgaat gctctcgatc acgttgatct agatacctta aaactgttct
    14881 cgattgagaa ccaaaccgag ctctagtcta tttagaccag cgtggtcttg agtgttgcca
    14941 ggccatcgac atgtccttct agaacatcac cacgagatac tgcagcaaca cccgctgggg
    15001 ttcctgaaaa tattagatca ccaggcatca aagtaaataa ggtcgataga taagcaatcg
    15061 tatcgggtac gttccagatc atcatcgcta aatctccgtc ttgcttcacg tctccgttta
    15121 cctttaaatg aatccgtccc ttcgcaagat gtccgcattg gctaactggt actagcgccg
    15181 agcaaggtgc tgattgatca aatgctttac cagtatccca agggcgcccc atctttttag
    15241 cctcgccttg gagatcacgg cgggtcatat caagaccaac tccataaccc cagacatgct
    15301 cgagtgcttt atccgcagta atgttcgaac ctcccttgcc gagtgcaaca accatctcaa
    15361 tctcatgatg cacgtctttc gataaagcgg gatacgccat actcttgcca tcagccacaa
    15421 ttgcggtagc aggcttcatg aaaaagaatg gtggctcgcg gtctgggtca tgccccatct
    15481 cgcgcgcatg gtcagcgtag ttacggccaa cacaataaat acgattaact gcaaatcgtt
    15541 tagtgtcccc ctgtacaggg agcgatggga tctctggggg attgataacg taagtagtac
    15601 tcatgattac tttctatttt aatttttaga ctcaattaat ccggctttat tacaaccatc
    15661 ttgtgtaatt tgatacgatt ttttctatgc tcgataaata tcaatgatac agtcccatta
    15721 tcaaagttta ttactcatcc agtcttgatc cggattgggt ggctctggag taaagcccga
    15781 agcacggact gcttcaattg cacaaaattc atctttatcg gatgcgtcgc cactaatacc
    15841 gattgcaccg attgcaacgc cttctttgtt tagaactaaa actccacctg gcgttggtat
    15901 aaaacgacca gacgaggcag ccgccagagc agattgaaat gttgggcgat cagataaccg
    15961 gtcgcggatt tgtctcgttg ccatgcccat tcctagggaa ccccatgcct ttccgatggc
    16021 aatatcagat cgcaatatgc cacagccatc ttcgcgccga aatgctacta gatggccacc
    16081 tgaatctaat acggctattg ctaagggcaa taattgatat tttttgcgga gttcaatggc
    16141 cttccctatt aatttctcgg ctagttcaag ctccagacta gagtaaaccc caattaaacg
    16201 ctcggccata agaattcctt aaaaaaagga tatttaaaca cgtcattctt aactacccaa
    16261 agcaatattg ttggattaat ataaatccca tcaaatgcaa atcattatgg agacccatgc
    16321 tttcagctac agacaatgcg tatctcaata ccagtgagcc caatactcca atgggaaact
    16381 atctacgttg ccactggcat ccggtagccc tttcagagga agttgcaaag ccagattgcg
    16441 cgcctattcg agtcaaggta atgggtgaag atcttttact ctttcgcgat agcaatggaa
    16501 agactggttt gattgaacca ttctgtgcgc atcgaggcgc agatcttttc tttggtagaa
    16561 atgaagactg tggtattcgt tgtatctatc acggatggaa atacgatata catgggaatt
    16621 gtattgacat gcccaatgtt cccaaggatg ctgcctatca cggtaagata aaaattaagt
    16681 cttatccgac tcaagagttt gctgatatgg tttgggcata catggggcct ccggatatac
    16741 aaccgattga agtcccacaa cttgaggcag gtcttgttcc ccccagtcac cgttatgtca
    16801 caaaacgttt ggttgagtgt aattggacgc attctatgga aggcgctctt gatactgcgc
    16861 acttttcttt cttacatatg ccagcacctg catatcgcaa agatgacagc tcaaatattg
    16921 ctgcggacga aagtcgtatc cgctggttaa gaaatgatcc tgcgccgcgt tttaagatca
    16981 ttgatcatgc agttggattt ttaattggcg gagccagaaa tgccgatcca ggggatcact
    17041 attggcgcct aacccaatac atgattccca ctcactctat tacaccatct gcaatgccta
    17101 atgagaccta ctatggttac tcatgggttc cgattgatga tcatcactgc tggatgtatg
    17161 tctatgcatg gcaccccgac gtagcaatcc cagatgagga gaggaaaaaa tacattaagg
    17221 gtggttacgg tcaatttgca gaattaggac ctgggtatgt gccacttcga aatcgtagta
    17281 atacttatct aatgagtcgt gaagaacaaa aaacaatatc ttttacaggc atacgaggta
    17341 tcgctgaaca agatcagatg gctcaagaaa gtcagggata tgttattgat cgtacaaaag
    17401 aaaatttatc tcccacagat gtcggaatta ttcggtttag aaagctatta ctccaagaag
    17461 caaaagcatt tgctgctggc aaaaggccag atgcgcccaa tcaagcaaaa gaatattgtg
    17521 ttcgcggtgg aggggctcat acgaaggcaa gtctagcact tgagcaagta atggaagaac
    17581 gttttggttc aatcactgga aaggttaatc atgcaaataa ctaatttttt tcgtttttta
    17641 ggtattggtt tagtaccttt attttttagc tttaatttaa gtgcgcaggc ttatccaaat
    17701 aaacccgtta ccgtaatcgt tccgttttca gctggtagtg caagtgatgt cgttacacgt
    17761 gtgctgcttg aaaaggtaag cagtaatacg aatactcgct tcatatttga taatcgacct
    17821 gctgctggag gaaacgttgg aaccgctgcg gttgtaaaag cagaaccgga tggatacacc
    17881 attggtttaa gtacttcagg cccgctggcg gtcaacaaaa tattaaatcc tgatcttggc
    17941 tacgatccag aacgtgatct acagccaatt gctctttttg ctatcttgcc aaacattatc
    18001 gtggttagct caacactgaa tatcaataat ctctcccaac ttaacgacta tatccgtaag
    18061 acacccgatg tggcttacgg ttccgttggc aacggcagct cacaacattt agcaggtgct
    18121 tactacgaac aattgctggg cgcaaaaatg acccacgttc cctatcgtgt aactgcaaac
    18181 atggtgactg atttagttgc tggccgagtt ccgattagct ttcaattact gcctaatgta
    18241 tctggtcaaa taagtgctgg cagagtcaag gctatcgctg ttgcatctaa tcgtcgccta
    18301 cctgctttac ctgatgtacc cacggcgaca gaagccggga tcaagggcta tgattcggca
    18361 gcatggttcg ccttgatagc ccctaaagct actccgaaag ctattgtgga taaattaaat
    18421 aaagatgtta atcaagctct agctgatcct gcggttcgtg ccaaatttgc agagcttggt
    18481 gccgaacccg tatctggacc accagaagat ttgacgcaac tcatggcttc cgatattaag
    18541 aaatggacag ccattatcaa aaaaggtggt attactatcg ataaacagta atcaagacat
    18601 catctgcttg agctgttcca taaataactc ttcac
//
LOCUS       DDPQ01000082            3661 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_25253,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000082 DDPQ01000000
VERSION     DDPQ01000082.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_25253"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1153..1234
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1359..1459
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          873 a          793 c          776 g         1034 t
ORIGIN      
        1 gttaagtgca tgcgttcatg gcgaccattt tgctggggat tgcccggttt aatccgttcg
       61 atgccaatgc ctaagcgcag ccaccacacc gacagactcg agaggccata aagcgcatta
      121 gcgcaagcaa agggcacgcc attgtctgag cggatactgt ctggtaaccc atactcctta
      181 aagagctgct caaagactgt gatggcctgc gcttcacgag tactctctaa gccctcacag
      241 caaatgaggt agcgcgaggc atagtcggtc accgttaagg ggtagcagta ctggcggttc
      301 cccagcataa actcaccctt gtaatcggtg caccacaaag cattgggggt cttcagatcc
      361 gataagttgg tccccgtgag cctagggtag tggcgtctgc gtttcttttt gactaggccg
      421 tggcgatcaa ggacggcgtg tacggtgctc ttggcgggta atgccagctc cccatagtgg
      481 gtgcgcaagc gctcccgcaa tttaggggca ccccaggtgg ggaactcgcg tttgagcctc
      541 acgatcaatt gctcgacctg aaagggcagc cgattggctt gccgaaaggg tcgtcgactg
      601 cgatcgctaa aggcttctag gccactatct ttataccgct caatgatctt atggccagtc
      661 acccgggaaa taccaaactc ggcacataag ggggcaatct tctcaccatc taaaaaacgc
      721 gacacaaacc ggagcttttc atccatcttc gtactttcat tccagggcat agagtggtcc
      781 tcctatgcca aaaactgtaa agtatgtgtc cggtataaag tgttaacgat gtgtcaggat
      841 ggacacaaac cctcactaga aatggtgggg gttttgtctt taggttggcc tgattttctt
      901 ctgtaattgc tcagtcatac atcgtcacac caccaccaat ctttttcatc ccgttcgtac
      961 tcaaggtcag gatctgatgg tctcttctct gattctttat gaatagaaga gttctgtttc
     1021 tgctcttctt tgagtttacg agagcaatat tgcggtctac ctagtatttc cggatcactc
     1081 attcatattc ctatatggct acacaaacct aatttgcatt gcaagggttt ctgtatttga
     1141 gtttactgat atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcaac ataaaaatgc ttaataaaat
     1261 taactcaatc atctaccgtc aaaacccaat acagattctc cttgtttggc tttatctttt
     1321 acccctagat tagcactttt ataagaattc aataactann nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnna aattgaatcg tcacaaactc acctaattct tttgcccacg
     1501 ccatggcttg gtctaatgta ttaaattcgt atgtttcgga gttatgttgt acggtaatca
     1561 acttagattt gcttaaatca aaaattttat aacttaatat atgggcttgt taaggtgata
     1621 atatttctag atattactga tctattcaaa aaagaacatt ttcatgaact tcatcagacg
     1681 ggctcgaaag ttttatgacg aaattgtaag tttaatcaag gcttatgctc gctaagtgag
     1741 caattttttc taagccctag tcccaggttc aagtccggct gaccccacca tacatggtta
     1801 agacttatct ccattccttt ttaaggctct agatgcaatc tcaatttgta atctcgatcg
     1861 tcctagccag tttaggggcg attctctttg ctgctaaagc catcgtggtg aaattttcct
     1921 atcagtatgg cgcgacagcc gatgttgttc taacacttcg aatggtgttc tccttgccta
     1981 ttttctggtt tgccgtctgg tggagccagc gaaatacctc attgcaaccc ttaattcgaa
     2041 aagatgttgt taaggttttc ggtatcggcc tcttaggata ttttttatca gcttatttag
     2101 attttctagg ccttcaatat atttctgctg gattagagag agtgatctta tatctaaccc
     2161 ccgccatcgt tttagtcttg tctaaatttc tgttaaagaa agaaatcgat cgtcggcagt
     2221 attgggcgat ggcagttgca tatctcggca ttgttcttgt gtttatccac gatattgagc
     2281 taaatggtag tggtgcggtg gcattaggaa gtacgctagt ctttttggca gccgtggtgt
     2341 actcgttcta cttaatcttt gcgggtgaat tggttgggcg tgtgggtagc attcgcttag
     2401 tggccttagc aagtgcttcg gcaaccattg cagcttgtct ccagtctctt gtcttggatt
     2461 tcaatcagtt atttattcaa actcctaatg tttatcagct agcatggatt aatgcactat
     2521 tttgtacgtt tataccaatg gtatgcatca tgatggccgt tcaacgtatt ggctcaagca
     2581 tgacagcgca ggctgggact atcggtcctg ttggcactgc ctttttaggg tggtacttct
     2641 tggatgaaaa gattagtact cttcagcttg ttggtatcgc tgtggtgttg atcggaattg
     2701 ccatcttgct atcgattggc aataaatcga atataagctc agcccctacg gaataagcat
     2761 ttatcgtttg aaggtcacta cgtgatcagc acccaagcgg ataccaattt tctctccgat
     2821 cgcatgattg tgatggctgg gtacaaatgc aaaaacttct tgtcccgaat ggagcttgag
     2881 agtatataaa aaatccgcac ccctaaacgc tttgcgtacc acttccgctt gcatgggact
     2941 ctcatcgtcg tgttgaatat catcggcgcg taagagcaca tcaatctgtt tgccagggga
     3001 aatatgttcc ccatcatcta gttgtagnna ccccgcgtcc aataaattca gcgacatagc
     3061 gatcaatcgg tttgtgataa agatcgtaag ggctatccca ttgcacaatc ttgccattgt
     3121 tcatgacgcc aatatgatca gcgatagcaa aagcttcaaa ttgatcgtgt gtcacgagta
     3181 aggcggttac attgtttgct ttgagaatat cgcgcatctc gtcagccaat cgctcacgta
     3241 gatcaatatc tagattcgag aacggctcat ctagaagaat taaatccggt tcgggtgcca
     3301 ttgctcttgc caaggcaacc cgttgctgct gtcctcccga aagctcatga gggtaggccg
     3361 ctgctttgtc ttgcaaggat actcgctcta accattgcat ccccgtttgt ttacgtgtgc
     3421 tgatggagcc ttcacgcaaa ccaaagatca cattctcaag aacggtgagg tgaggaaaaa
     3481 gggcaaagtc ttgaaagacc atgccaacct tacgatcggc agggggaatg cgcacattct
     3541 ttgagctaac cgttttgcca tgaatcttga tctcacccat ttgcaagggt tcaaagccac
     3601 aaatagcgcg caaaacggtt gatttaccac agccagacgg gcccagtagg caggcaatat
     3661 c
//
LOCUS       DDPQ01000083           10418 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11167,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000083 DDPQ01000000
VERSION     DDPQ01000083.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10418)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11167"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2438..2463
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4357..4383
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2639 a         2314 c         2576 g         2834 t
ORIGIN      
        1 attgagtcgc cattgttgat tggtctctaa tgtaccgtag agacgccatt gaaaagtttc
       61 acaccgaatt ccttcaaagt aagcattttg actgccactc ggattcgtaa tcacaacgat
      121 atagcgggtt acgccatcgt taccaatctt gatagattct gtatcaaccg aaaatttaaa
      181 gacagtagtt tgagacaccg agaagggtac taaatatttt ttattgggcg gattaagtgg
      241 caagggggtc gaaccctcct taaacaccgt aggcgcatag gggtctgtac catcaatcag
      301 tggatcagag aagcatccag caagggtaag tgtcagagcc agggtagaga gcgtgcgttt
      361 catgatgtgt gtgaggattc gaatggtgct ccccaaacaa gctgatgcat tgcagtgcta
      421 gtggcaataa agcgtgcaag ttcggagagt gccaattgat aaacctcgcg cttaaaatca
      481 atcacggtct caagctcaat ccaataggga tgccagcgcc acgcatcaaa ttctggatgg
      541 gcggttgctt tgagttggat ttcatgatct tgtcccagca ttcgcagtag ataccaaatt
      601 tgcttttgac ctttataact aacgcgctga ttacggttga tgacttgccg acgcaagaac
      661 tcctcgggaa cgtcgtaacg aatccagtcg cgggttctac cgagaatctc aacatgatgc
      721 ggaaataagc caacctcctc atgcaactcg cgatacatcg cctgttctgg actttcacca
      781 tgctgtattc ctccttgcgg aaattgccat gagtgttgtc ctatgcgttt gccccaaaat
      841 acttcgttac gagcattgag gaggacaatc ccgacattag gtctgtagcc ttcgcggtcg
      901 agcataatcn ngccccagcc gatgctgagg tagtgtccca ccagcttatg gtgcgggctg
      961 gcttgatccg taaattaagt gccggtattt ataactatct ccctctcggt ctgaaggtaa
     1021 tccgtaaggt cgaaaatatt attcgggaag aaatgaatcg tgcgggtgct attgagctct
     1081 taatgcctat cgtgcaacct gcagagttgt ggcaagagac tggacgttgg gaaaaaatgg
     1141 gccctgagtt actgcggatt aaagaccgtc acgagcgtga ttttttgatc cagcccacct
     1201 cagaagaggt tatcactgat ttagctcgca ctgaaattaa gagctataaa cagctaccta
     1261 ttaactttta tcagatccaa accaagttta gggatgagcg ccgcccgcgt tttggaatca
     1321 tgcgcggtcg agagttcagc atgaaagatg cgtattcgtt tgatcgcgac catgatggtt
     1381 tgaagcgttc ttatgccctg atgtttgacg cttatgtccg gatctttaag cgtatgggct
     1441 taacatttcg agctgtcact gctgacaatg gggcgattgg cggttctggt agccaagagt
     1501 ttcatgtgat tgcagacact ggcgaggatg ccattgtgta ttgccctaac tcagattacg
     1561 ccgctaatct tgaagctgca gaatctttat ctttagttac aaaacgtgat gcacctaaag
     1621 aagtaatgaa aaaggtttcg acgccagaac aaaagcgctg tgaggatgtt gctaggtttt
     1681 taaagatatc cttaacccag actattaaat ccttggtgtt agctgtagat caagagcatg
     1741 ggccagcaac gttctttatg cttttattgc ggggcgatca tgagctcaat gagatcaaag
     1801 ccagcaaggt gccgggcttg acagcatttc gctttgccag cgatgaggaa gtaaagactg
     1861 cttgtcattc acctgtaggt tatttgggcc ccgttggttt gagctctaat gtgcagattg
     1921 tggctgatcg caccgttgct aatatgagcg actttgtttg tggtgccaat gaagcaggct
     1981 atcacttgac cggcgtgaac tggggccgtg atttaccgga acccattatt gctgacatac
     2041 ggaatgcagt agccggcgat ccatcccctg atggtaaggg ggttgtggat atttgtcgtg
     2101 gaattgaagt aggccatgta tttcagttgg gaacacgata ttcagaagca atgaagtgta
     2161 cttaccttga tgagactggt aagtcgcagc cgatggtgat gggctgttat ggcattggtg
     2221 tgacgcgttt actgggtgct gcgattgaac aaggtcatga tgaaaaaggg attttgtggc
     2281 cgatttcgat ggcccccttt gagcttgtta tctgtccggt tggctatgac aagtcagaat
     2341 tagtaagaca agctgcggat gatctgcatc agcaattaca aaacgcgggc atagatgtgg
     2401 ttttagatga tcgcggtgag cgtcctggag caatgttnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnaaagaag gtaatgtcga gtttcagggg cgtcgtgaaa gtactgctca gttagtgccc
     2521 gtgaatgaaa tcacagagaa ggttgttgcg atcattcggt ctgcaaagga caatcttctc
     2581 tagagttact ttttaaaaag tccgccaagc cctttggcag ctccacgagc agcaagagag
     2641 cccatcatct tcgccatctt gccgcccttc atttgtttca tcatggcttg cgtttgttca
     2701 aattgagtaa gtaagcgatt gacctcttga acttggactc ccgcacctgc agcgatacgt
     2761 tgcttacgct ttgcctttaa taactctggc ttggcgcgct ctaagggggt catactgtcg
     2821 ataatgcctt gcatgcgctt catttgttta tcggccatcc ccaaattggc tttactcgct
     2881 tgttgagcta tttgactggg cagcttatca agtaaattcg ccatgccacc catgttttgc
     2941 atttgcatga tctgttcacg gaaatcacca agatcaaaac cacccttttg aatcttgctg
     3001 gcaagctttt gggcttgctc cacattgacg ttttgttggg cctgttcaac cagagccaga
     3061 atatcgccca tgcccaaaat acggctggtc attctctccg catcaaaggc ttcgagccca
     3121 tccatttttt cagaaacacc aatgaactta atcggtacgc cagtgatgtg acgcaccgag
     3181 agcgctgcgc cacctcggga gtcgccatct aatttggtca gaatgacccc tgtgagtgcc
     3241 aatgcttcat ggaaagcttt agccgtattc acggcgtctt gaccaagcat tgcatcgact
     3301 acaaagagag tttcaatcgg gtttagattg gtatgcaagg atttaatttc ttgcatcatt
     3361 tgtgcatcaa tacctagacg accggcagta tccacaatca agacatcgtg gtaatggcgc
     3421 tttgcccagt caagcgccgc ataagcaata tcaatgggtt tttgttcgac ggtgcttgga
     3481 aaaaaatcaa ctccgacttg tgcgctaacc gtcttaagct gttcaatcgc ggcaggacga
     3541 tagatatcgc aagagaccgt taatactttt ttcttatgct tctcttgtaa aaacttggca
     3601 agcttggcaa cagatgtggt tttaccggca ccctgaaggc ctgccatcaa aatcacagct
     3661 gggggttggg tggctaaatt taacttacca ctctgcccct catcaccccc catcataaga
     3721 gacagctcac gctgtacgac cccaactaat gcttgacccg gggtgaggct tccaaccaca
     3781 tcctcaccaa gggccttgaa cttgatttgc tccatcaggc tcttcacgac agggagagca
     3841 acgtcagctt ctagtagggc tagacggatc tcgcgtaaca tctctgcggt gttgctttct
     3901 gtgaggcgtg cttgaccccg catggtttta accacacgac ttaagcggtc ggtcaggttc
     3961 tctagcattt atcgattaga ctttcgaaat gactatttta ggttactcag gatttggctg
     4021 gttgccatcc attctttacc tcttattgat cgtcgttttg ggctatcgcg accaaaaacg
     4081 acccgagacc ccatttttta tcggtttagt gcaaatcttg atattgatcg tcttaatcat
     4141 tcacggaacc ttattgcatg attccatctt taacggtgat acctttgtat tcggctttgc
     4201 tcaagacctt tctttaatgg cctgggtagg actcagtttt tattggattc aaagcttctt
     4261 tttgcccatc tctagtctgc gcttgatggc gattagtatg gcgatgtttt gtgctgtctt
     4321 acctgactta ttccccggat cggtaatttc ccagcgnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnntgcgatg ctagcggtgc tcatgaatgt ccaagatcgg gctttacgca agggtatggc
     4441 aaatggcatg gcaggctcac ctacttgggt cgagagtttg ccgcctttat tgaccatgga
     4501 aagcttttta tttcgcttgc tttatgtggg ctatgtttta ttaacattga ctgtattttc
     4561 gggactattt ttctcgcaag agttatttgg taagccctta gtgtttgacc acaaaacagt
     4621 atttgcgctc ctctcctggg ctttatttac agggcttgta gtcgctcgta ttcgagttgg
     4681 tctgcgaggg ccaagtgcgg tgcgatgggt cttgggtgga tttcttgccc tactcctgac
     4741 ctatgcggga actcgttttg tcgctgaagt tattttgcag cgagtatagc cttgcttaag
     4801 tgggcattat tggttttagg gctggggctt gcttattggt ggctgatcgc taaaaagaaa
     4861 agcaaggctc agacagctga taaaaagatt cggcaaggag aaaccccctt aacgagctcc
     4921 cagcggatgg tctcctgttc gtattgcgat cttcacctgc cccaaaacga tgcgcttccc
     4981 caagacggac gttattactg ctgcacagag caccgcgata gcattagcca aaagggctgg
     5041 tggggtaaag cgcaatggtg cgcatcgaca aattttgacg aacgaccaga ggatatagcc
     5101 atcaatttgg cggtgatcca tcacattagc ttgccgcccg gtgaatttgg agggcggaat
     5161 attgttgatt tctttcagaa taatttagat cccaaggcgc atccctactt tgagcagatt
     5221 gctaaccaaa aggtatcgag ccactttctc atagaccgga ggggaaaaac aacacaattt
     5281 gtgtcccttc ataaacgcgc ttggcatgcg ggtgtatcgc aattttgtgg acgcgagaaa
     5341 tgcaatgatt tttcgattgg catcgagtta gagggcgatg gagatacccc atttacaaat
     5401 gagcaatacg cgagcttggc cgaggtgacc ggggttttgc ttaagaaata tccagacctt
     5461 cagtttgcgg ggcatagtga tattgctcca gagcggaaga cggaccccgg accatccttt
     5521 gattgggtgc gcttaacaaa aatagcaaag ctatcagaag agaaattacc ctttgggagc
     5581 gcttctcgct aagcattagt tcaatgggta agcgtttgct tacttagtat cagcagaaca
     5641 gattttttcg aaattaatag ggaaattacc aataaataga gtcagaaaag acttaccaaa
     5701 tttaaaatat ttgcctatac ttagtgtcta gggcgataga aaaacactac atctagtgtt
     5761 ttttctgtaa attagccttt tttcaataaa aatatacata tagcaggagt aatatgacct
     5821 tcgccgatcc tcaagctgca ggccaatcaa gtccagtaaa ccaaggcggt ctcggcgcag
     5881 gggctagttt gacccaaacc ccatccgcga attttgtagc gggtggcgtg ggtctggcgc
     5941 aagcaacgca actctcggac tacaaaatta ttcggcgtaa tggttctgta gtcgcatttg
     6001 agccatccaa aattgcaatt gctgttacca aggcattttt ggctgtgaac ggaggccagg
     6061 gagcagcttc agctcggatt cgtgagcagg tagaacaact aacccaaaac gtggtgcgcg
     6121 ctttattgcg cagccgtccc aatggcggca catttcatat agaagatatt caagatcaag
     6181 ttgagctgac cttaatgcgc agtggtgagc ataatgttgc acgcgcttat gtcctttatc
     6241 gcgagaagcg taatcaagag cgcgctgccc agcaaagtgc aagtcaagat caggctgccg
     6301 atgccgcatt accctccgtt caggtcaacg ataatggtgt tagcaaaccc ctcaatgttg
     6361 ttggtctcag aacggtgatt gaggctgcat gtgaaggatt gggaaatcat attgatgcta
     6421 gcccgatcat gagcgagacc attaagaact tatacgatgg cgtgccaatg gcgcaggtct
     6481 atgactcggc gattttggct tcgcgaactt tgatcgaaaa agatcctgct tatagtcaag
     6541 ttactgcacg tattttgatg cacgtgatcc gtaaagagat tttgggccgt gaggttttgc
     6601 aaggcgatat gcaagccgag tacccgaatt atttcccaca atttatccgc cagggcatcg
     6661 aagctgaact gcttgatccg cgcttactcg agtttgattt agctaagatc tcatctgctc
     6721 ttagcgccga cagagatttg cagttcaatt accttggcct gcaaaccctg tacgaccgtt
     6781 atttcttaca cattgaggat catcgtattg agatgccgca ggccttcttt atgcgggtgg
     6841 cgatgggtct cgccttaaat gagccagagc gcgagcgtcg tgccattgag ttctacgaga
     6901 ttctttctac ctttgacttt atgtcaagca ccccaacttt gtttaactcg gctaccttac
     6961 gttcacagtt atctagttgc tacctgacga ccgttgacga tgaccttgat ggtatctacg
     7021 aagcattaaa agagaatgct ctcttgtcta agtttgcagg tggcctaggt aatgattgga
     7081 ccaatgttcg tgctctagga agtcatatca agggtacaaa tggaaagtca cagggtgtgg
     7141 tgccattcct taaggtggtc aacgatacag ccgttgcagt taatcaaggc ggtaaacgca
     7201 agggtgcagt ctgcgcctac ttagaaacct ggcatttaga cattgaagag ttcttggaat
     7261 tacgtaagaa cacgggcgat gatcgtcgtc gtacccatga tatgaatacc tcgaactgga
     7321 ttccggattt gtttatgaag agggtgatgg aaaacggtga ttggacttta ttttccccat
     7381 caaccacccc ggatttgcat gataagtatg gcaaagcctt tgagcaagct tatgttgctt
     7441 acgaaaagcg ggcagaggct ggcgagatta agccatcgaa gaaggtgcca gcagttcaga
     7501 tgtggcgcaa gatgttggcg atgttgtttg agaccggcca tccttggatt acgtttaagg
     7561 acccttgcaa tattcggagt ccacagcagc acgttggcgt ggtgcattcc tcgaacctat
     7621 gtaccgagat caccctgaat accaacgaga ctgaaattgc ggtttgcaac ttaggttcgg
     7681 tgaacttgac tgcccatatg accaccgatg caaatggtca gatggtcttg gatcacacca
     7741 agctcaagaa aaccattcag accgcgatgc gtatgcttga taacgtcatt gatattaatt
     7801 actacgccgt tgccaaggcg cgcaattcaa atgtgaagca tcgcccagtg ggtttaggca
     7861 tcatgggctt ccaggattgc ttacatatgc agcgtattcc gtatgctagt gatgcggcag
     7921 tgaagttcgc agacacttcg atggaagccg tttgctatta cgcctaccag gcatctagtg
     7981 agttggccaa agagcgaggc gcttacagca gctatcacgg atctttgtgg gatcgcggca
     8041 ttcttcccca agacaccctc aaaatgcttc aggatgagcg cggtggctat gtggaagtcg
     8101 atcagtccag cactatggat tggaatgcat tgcgtgctca gattaagcaa cacgggatgc
     8161 gtaactctaa ttgcgtggcc attgctccaa ctgcaacgat ttctaatatt attggggtat
     8221 cggcctgtat tgagcccact ttccaaaatt tgtttgttaa gtcgaatcta tctggtgagt
     8281 ttacggttgt gaacgactat ctcgttcgcg atctcaagga ccgcgggctt tgggacgagg
     8341 tgatgattgc tgacctcaag tattttgatg gcactttgtc aaagatcgat cgggttccac
     8401 aggatttgcg tgatttatac gctacggcct ttgagcttga gccaagttgg ttggtcgagg
     8461 cggcctcccg tcgccagaag tggatcgatc aagcgcaatc cttaaatatt tatatggcag
     8521 gtgcctcggg caagaaattg gacgatacct acaaactcgc ctggttgcgt ggtttaaaga
     8581 ctacctacta cctgcgtact atggctgcta cacacgttga gaagtcgacg gtagcaacag
     8641 gtcagctgaa ctctgtttcg aatggaacag aaacagcccc agctctagcg gcagatgggc
     8701 cggtatgcac gatgcgccct ggcgaccctg gatttgaaga atgtgaagcg tgtcagtaac
     8761 ccttgctgta attaagtcta taggaagaat taggagaaga ttatgttgaa ctgggacgat
     8821 gaagttgcac ccgcgttagc gaaggcgggc tttgctccgc ccccatcgat gcctgtgccc
     8881 ccagcgcagc cacagttagc acctgttcaa gcggatagcg ttgctatggc accgccagcg
     8941 caaccagtca agcctgttgc agaagcgatt gagcggcgtg tgaacgtggc cgataaacgg
     9001 gtcattaatg gcacgacgga cgttaaccaa ttggtaccat ttaagtacaa gtgggcttgg
     9061 gagaagtatc tggcgggttg tgcaaaccat tggatgccac aagaaatcaa tatgaaccgc
     9121 gacattgccc tatggaaaga ccccaatggg cttacagagg atgagcgacg catcatcatg
     9181 cgtaacttag gatttttcgt tactgctgac tctttggcgg ctaacaatat tgttcttggt
     9241 acctatcgcc aaattactgc cccagagtgc cgccaatatc tgttgcgtca ggcttttgaa
     9301 gaggctattc atactcatgc ctatcagtac attgttgagt ctttaggcct tgatcaagct
     9361 gaaatcttca atgcttatca cgaagttccc tcgattcggg ctaaggatga gttcctaatc
     9421 ccctttatcg atgtactcac agatcctgct tttaagactg gaactttgga aaatgatcaa
     9481 aaattgttgc gctctttgat tgtttttgcc tgtgtgatgg aaggattgtt cttttatgtt
     9541 ggttttacgc aaatcctcgc aatgggtcgc caaaacaaga tgactggtgc agcagagcag
     9601 tatcaataca tcctgcgcga tgagtcaatg cactgtaatt ttgggatcga tttgattaac
     9661 caaatcaagc tggagaaccc acagctatgg acttctgcgt tcaaagagga gatccgtagc
     9721 atctttgaaa aagcggttga gttggagtat cgctatgccg aggatacgat gccaagaggg
     9781 gtgcttggat tgaacgcgcc aatgttcaaa agctacctta gattcatctg taatcgccga
     9841 tgcatgcaaa taggacttga ctccatgtat ccaaatgaag agaatccatt cccatggatg
     9901 tcagaaatga tagatctgaa aaaagagaga aacttttttg agaccagagt gattgagtat
     9961 caaaccggtg gtgcgttaaa ttgggagtag acacagccta aattaacgcc ccgtccggca
    10021 aacataggag ataggacggt tgattagtac tcgcagtcga ttaaaacagg cacaaatgcg
    10081 aaaacccagc gtacagggtg accggatctc tttccccatt ttttttaccc catttaaaaa
    10141 gccgcttcct gcaaaggttg cggctttttt tccaagattc attagcgtgc agcagcaagt
    10201 ttcaagccgc gcgccgatga atagctcgct cgtttcctat atggcaccgc aaggtgctct
    10261 acagagtaat ggtcgggttt tcttaaacag tagtttgttc taatcctcac gtgaaggaga
    10321 atcactatgg caatcgccaa aaagaagtct gctgcaaaga aaccagctgc taagaaaaaa
    10381 gtagctgcta agaaaccagc tgccaaaaaa gcagctaa
//
LOCUS       DDPQ01000084            4971 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_9385,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000084 DDPQ01000000
VERSION     DDPQ01000084.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_9385"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3463..3567
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1376 a         1148 c         1008 g         1334 t
ORIGIN      
        1 tcgacagttt agcaaagatt caggtttagg gctttaattg cccaaatata ggtgagctaa
       61 ggcctatttg tgagctgggg taaagccttc tggaggtaat aaaacattga ccagagggtt
      121 aggaaggccg caacccaaat aagccaggtt ccaagttggg cgctatgtat accaaatagc
      181 tgcccgttaa atagtaggaa tgggatcgca attaattgtg cggtggtctt taacttcccc
      241 accatatgca cagccacact tcttgaggcg ccgacttgag ccatccactc tcttagcgca
      301 gaaatagtaa tttctcgtcc gataattact agagcaaccc agacctgaac gcgatccata
      361 ttgagaagca ccaataaagc agccgccaca attaatttat ctgccactgg atctaaaaac
      421 tctccaaagg cagactcttg tttcattcgc cttgcaagaa atccatctag ccaatcagta
      481 atcgcagcga agacaaataa agcggttgca aatacatttt tttcttcaat gctgagccaa
      541 ctactgggta aataaaaaat acctaccaat aatggaatgg cagcaacgcg caaccaggtc
      601 aatgcaattg gtagattaaa tggcatattt agagaataaa cgattcgtaa tgctatttag
      661 tgaagttggc gatagatttg ttcagccaag gtatttgaga tcccttctac gctagataac
      721 tcttccacac tcgcgttcgc cactccttta atgccgccaa agcgggccag caacttctgt
      781 cttcgccttg caccaattcc ttcaatttct tctagccgtg agaccgttcg agccttagct
      841 ctctttgcac gcattccagt aattgcaaaa cggtgcgcct catcccggat ctgtgcaatc
      901 aacattaggg cttggctatc ctgacccaag aataatgact ctctgccgtc agcaaagatg
      961 agggtttcta aacccacctt acgatcctcc cccttagcaa ccccaactat caaactgata
     1021 tcaatgccta gctcactaaa gacttgccta gctatctcaa cctgtccctt gccgccgtca
     1081 atgaggacta cttgggggtt cttatccact ggggtttctt gataattaga gtaacgccgg
     1141 attaagacct gccgcatggc agcatagtca tctccaccag taattccggt gatattgaag
     1201 cgacgatatt cacttgattg catttcattt ttggcaaaga ccacacacga tgcttgagtt
     1261 gcctctccgg atgtatggct gatatcaaaa cattcaatgc gaagttgatc caagtgttca
     1321 agatcaagat ttaatacatc tgcaagggct ctcactctag ctaattgccc accagtctct
     1381 gagatgcgcc ttagtaaagc aattttggca ttcccttcgg ccatagaaag ccaatgcttt
     1441 cgttgtcctt gaggttgatg cagtaactgg accttacgcg ataagcgctc ggtcaaacct
     1501 tgtaataaaa catcaactga ttcgcccttc aaagctacat tagtaatcaa taccgagggt
     1561 aacaatgaac ttggctctgc attaatatcc acatcgagat aatgctgctg aataaaggtc
     1621 tccagaattt cactgggatt ataggtagca tctgaactag cctttatccc tcttgggaag
     1681 taagcccgat ctcctaagtg ccgacctcct cgtaccattg ctaaattgac gcagatattt
     1741 ccatccatga tctcggcagc caaaatatcg acatcaccct ctccctctgc gacgacatcc
     1801 attgcttgtt gttgcaagac gcttgagaga tccgtaatcc ggtcccgaat aactgctgcc
     1861 atctcaaact ccatcgcagc actatgctct tccatttcta cttcaagatc agctaataca
     1921 gctcgatggt ttccttctag aaatcgtagt gcctgttgca catcttgcga atactgttct
     1981 ttgctcaaca tgccaacaca gggtgcgcta caacggtgaa tttgatgcaa taagcaagga
     2041 cggctgcgat tcttaaaaac tgggtcctca caggtacgca agcggaatac cttttgtaag
     2101 atttgtacgc tattacggac tgcccatgaa ttagggaatg gtccaaaata ccgattgcgt
     2161 cgatcgacct tgccgcgata cgaggcaagg cgaggataat catggccact cagcatcaaa
     2221 tacggatagg acttatcatc ccgaaataaa atattaaatg gcggggtatg ttccttaatt
     2281 aagttattct ccagaatcaa tgcctctgtt tcagtccgcg ttacagtgat ttcaaagcga
     2341 agaatccgtt tgaccatacg ctcgatacgg ggcgataact gagtgcgctg aaagtagctc
     2401 gatacgcgct tcttaagatc cttggccttg ccgacatata agatgtgtcc agcctcatca
     2461 aagaagcgat acacacctgg gagaccaggt atgcgtttta gatctttttg aagggtttct
     2521 aggggcaaat ttgtcatgcg ttgggatatt ttctgccaaa tcgttgataa ctatggtgat
     2581 gctggtattt gctggcgtct agcccggtct ttggctgttc aacaccatca aaatatacgc
     2641 ctattttgcg atgaccttca taccctcaaa ctattaatga tgggtagcgg tgatatccaa
     2701 ggtattgagg ttttgccatg ggaggctagc tatgcaaata cacgacatgg gccagaaact
     2761 cccgatgttg tgattcaggc cttctcctgc gacctacccg aacgttatct gaattattta
     2821 atccttgccc cacaaaaacc gatcttgatc catttggaat atttaagtgc cgaaccctgg
     2881 gttgtggatt ttcatggcaa agcatctcct caaaataatg gtctccagaa atacttcttt
     2941 ttccctggtt ttcaaaaaga ggttggggga ctgttattgg atcccatccc cccaagcaga
     3001 caagatatgt gtccaaaaag tctttcctcg atttgggacc aatctcgtga aggagccaag
     3061 aagatcagta tcttttctta tccaggggca cccatcaagc aatggcttac tcagcttaat
     3121 caattggata tccccgttga tatattcatg gcttttggta acgctgaatt actcaacctg
     3181 caaaagcaag catggtcgaa cttaaaacta atctcgctac ccttcatccc gcaagatgat
     3241 tttgactatc tccttgccca atgtgacttc aatattgtcc gcggggaaga ctcttttgta
     3301 cgagcccaac tagcgggcag accttttatt tggaatatct accctcaaga tgatggtgcg
     3361 cacttggtta aattgagtgc atttctagac ctctatctca aagaagcacc cccagactta
     3421 agcaattcag ttactgaagc catggcatgg tctgacatcg atnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnncca caaaaggtta aaatcctagg tttgattaat
     3601 ttggcttcat gagccaagcc aatatagaag atattaggga ctgttatgaa aatcgctcaa
     3661 gaaatccgcg tcggcaacgt tgtcatgatc aatggtgaac ccatggtcgt ccaaaaacat
     3721 gaatacagcc gctctggtcg taactcagct gtggtcaaaa tgaaatacaa aaatttactg
     3781 actgccgcac cgaatgaagg catttataaa gcggatgata agttcgatat cgtgatccta
     3841 gacaagaaag aatgtactta ttcttatttt gctgatccga tgtttacatt catggatact
     3901 gaatacaacc agtatgaggt tgaaaaagat aatatgggtg actcgctcaa ttatttagaa
     3961 gaaggcatgg ctgtggaagt ggtgttctat gacggcagag ctatctctgt tgagttaccg
     4021 acctccgtag tccgtgaaat tacatacaca gagccagcag tcaaaggtga taccagctct
     4081 ggaaaagtac tcaagaatgc aaagatttca actggctttg aaatccaagt gccattgttc
     4141 tgtagcactg gcgacaagat tgaaatcgat actcgcactg gcgaatatcg gagtcgtgct
     4201 aattaatttg taatcagctt aaggcttttt aataaagcct tagcatgaat atactcagac
     4261 tctgtctgaa gagattccca agattggaca gcggctttcg gaaagagtct attaaggcgc
     4321 ttagcagact ctgctagttt tgactttgat accttgagct tactgagcat ttgatctgct
     4381 aggtctggac ggttgcaaat gagtactgca tcgcaacccg cttcaagagc taagtgtgcg
     4441 ccttgtacaa catcaccggc aatgcttgca ccttccatgg agagatcatc gctaaagatt
     4501 actccctcaa atttaagctt tttgcgtaag atatcttgta gccagatctt agagaatcct
     4561 gcgggcattg aatcaacttt tggataaacc acgtgtgcag gcattacgga tgccagacta
     4621 atatctaacc attcataagg tttcatgtca tcacgcgcaa tacgttttaa ggatcgatca
     4681 tcaataggga tatcaatatg ggaatcagcc ttagcccacc catgccctgg aaaatgtttt
     4741 ccacaatttg ccatatcgcc caaacgcaag ccttcattca gactttttgc aagtgcaaac
     4801 acaacttcag gtaaagaatg aaatgcgcgg tctccaatga cactgctatt tccataatca
     4861 agatcaagga ctggggtaaa gctgaaatca actccacagg acctcagttc gcttgccaga
     4921 atatatccgc atgccgtagc tgcttgcatc ccctcaatcg cagcctctat a
//
LOCUS       DDPQ01000085            4902 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_27809,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000085 DDPQ01000000
VERSION     DDPQ01000085.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_27809"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT         1350 a         1193 c         1160 g         1199 t
ORIGIN      
        1 ttaaaggctt caaggattgg catgccgaaa atagggctgc ctggggtgcg tgctgccggg
       61 ttaactacgt cattggcgcc aagcactaaa actacgtcag cttgaccgaa atcactattg
      121 atatcttcca tctcaaatac ttggtcatat ggaacctctg cttcggcgag aagaacgttc
      181 atgtggccag gcatacgacc agcgacgggg tggatggcat atttaaccgt tactccgtga
      241 tgaatcaatt tctcagtcaa ttctttaagg gcatgctgag cacgtgctac tgctaaacca
      301 tagcctggaa cgatgatgac ggtatccgca ttggtcatta agaaagcagc atcttctgct
      361 gaaccggttt tgtagttctt cggaccacta tcttctccac cggcagcagc ttcggcacca
      421 aagccaccca gtaatactgc aacaatggaa cgattcatgg ctttgcacat gatgtaagac
      481 aggatcgcac ctgaggaccc aacgcaggca cccgcaataa tcaatacggg gttatttaaa
      541 gtgaaaccga taccagcagc cgcccaacct gaatagctat tgagcatcga tacaaccact
      601 ggcatatctg cgccgccaat tggaataatc aaggtgacac cgagaacgag agcaatcgca
      661 cacatcacca agaaggctgc atgacttcca gtcaagaagt atgcaaaacc agcggcaacc
      721 atcaaaatag ccatgattag gttaagcaaa tgctgaccag aaaaactaac gggcttacca
      781 ctaactttgc cagatagttt tccaaaggca atgatcgagg ctgtaaatgt aattgcacca
      841 atgaatgcgc cgataaataa ctcgatcttt tgcgcgcccg aatgttcttg ggcaggatta
      901 aatactgccg caatcgcaat cagtacagca gatagaccaa cgaaagagtg cattagagca
      961 acaagctcag gcatcttggt catctggacg cgtttggccg cgattgagcc aatgatggca
     1021 cctgcaacaa tcgcaacacc aattaaccaa tagaccggtt taaagctcgg aattaaaaaa
     1081 gtggtaacga ccgctaaggc cattccaatc atgccgtagg tattgccttg gcgtgaggtc
     1141 gttggggagg ataaaccctt cagggctaag ataaagagta ctgaagagac tagataagaa
     1201 agggctgtga tatttaacat gattcgatct ctttttagtt accagccgct tcgttcttgg
     1261 gagctttttt cttaaacatc tccagcatgc gtcgcgtcac cataaagcca ccaaatatat
     1321 tgatcgatgc caggaacaca gcaagcgcac caatgaggct agtaagagtg atctcatccc
     1381 cattaataat ttcggtttgc aagagagcgc ccacgatgat gattccagaa atggcattgg
     1441 tcactgccat caagggggtg tgcaatgcgg gggtaacgtt ccaaacaaca tggtagccaa
     1501 caaagatagc caatacgaag acagtgatgt tttgaacagt taatagactt tgaatagcag
     1561 cagcatccat tttggttcct tcagaaatta attggtacga atggcttgac catcacggca
     1621 catcaaacaa gcggcgacga tatcgtcatc tgcagcagca ggaatcgcca gttgcgcttc
     1681 tttattaatg atgagtttca tgaaatcaag caaattacgg gcatataagg cagacgcatc
     1741 agcagccacc atgctaggta gattggtgta tcccacgatc ttcacgccat gatgattaac
     1801 caccttatca gcttgcgtga gtgggcaatt accagagcca ttgtcgcccc gacccgcagc
     1861 gatatcaatc accactgaac cgggtttcat tttgctaact gtatcggtat gtaacaagac
     1921 cggtggctta cgaccaggga ttaatgccgt agtaatcacg atgtcagctt gcatagcccg
     1981 ttcagcaacg agggccgctt gacgcttcat ccaagcctct ggcattgggc gtgcataacc
     2041 acctacgcct tgagcaatct cgcgctcttc atcggtctcg taagggacgt ctacaaactt
     2101 agccccaaga gactcaattt gctccttagc agcagggcgc acatcggatg cctcaattac
     2161 tgagcctaaa cgtttggcag tagcaatagc ctgtaaacca gcaacaccag cacctaaaat
     2221 gagcacacgc gcagctttaa cagttcctgc ggcggtcatg agcattggca taaagcgttg
     2281 atattcattc gtagcgacca tcacagcttt atagccagca atattcgctt gggaagatag
     2341 tacgtccatg ctctgagcac gggtagtacg aggtgccgct tctagggcaa aggctgtaat
     2401 gccttgtccg gccatggctt ggatgccggc attatcgaat ggatcgagca tgcctaataa
     2461 aacagcaccc gatttcattt gctttagttc agcagcctct ggtgagcgaa ccttcaaaac
     2521 aatctcagat cccaaggcat cggctgcaga gccaatactg gctcccacgg cctcataggc
     2581 tgaatcaggt tggctagcac ctacgccagc accagcttgt atcaagacct gatggccttg
     2641 gctaattaat tttttgacag tctcaggggt tgcggcaaca cgggtttccc cggccctcgt
     2701 ctccagcggc actccaatgc gcatggctta tctccaaaag caacgatttc taaaaaagga
     2761 cctattttaa taaattagcc atgccttgtg agctagcccg ctcagtctcc tgtatatccc
     2821 tttggccaga ccttgaccga gacttttaca atatgagctt gaaacaatta acgttatttt
     2881 cgcacttttt taatggttag ccccaatatc caaagccccg tcccccaaag ccctaccagt
     2941 aaggctttgg gcaactattc tgccccgcag aaggtagtag ttgggatgtc cgggggggtg
     3001 gactcttcgg tagcggcatg gcttcttaag caacagggtt atgaggtcgt aggccttttt
     3061 atgaagaact gggaagacga cgatggcgag gaatattgct ccgcccgaca agattggtta
     3121 gatgtggtct cggttgccga tatcttaggg attgatgtgg aggctgttaa ctttgctacg
     3181 gaatatcgcg aacgggtatt ttctgatttt ctaagagagt atgctgcagg ccgcacccca
     3241 aatccagatg tactctgcaa tgctgaaatt aagttcaagg catttttgga tcacgccatc
     3301 agcttgggtg ctaatttaat tgctacaggg cactatgcgc gcgttaaaca ccatgatcag
     3361 gaagtgcagt tattaaaagc ggttgatacg accaaggatc aaagttactt tttacatcgt
     3421 ttaactcaaa tgcagttatc caaagtttta tttcctctcg gagaaatcac taagaaagag
     3481 gtgagacaaa tcgccgcaga ccttggtctg ccgaatgctc gtaaaaaaga ttccaccggc
     3541 atttgtttta ttggagaacg acccttccga gagtttctca atcggtattt accaagagtt
     3601 cccggcccca tcaaaagtgt cgatggcaaa gtattgggtg agcatatggg cttagcgttt
     3661 tttaccctgg gtcaacgcaa agggattggg cttggcggta gtcaagacgg aactggtgat
     3721 gcttggtatg tggctcgcaa agatattgct aacaataccc tgtatgttgc tcaaggacac
     3781 gatcatcctt ggctacttag cgatacctta tcggcgatgg atgcatcgtg ggtagcgggt
     3841 tgcgaaccta aattaggaca atacagcgct aaaacacgct accgtcagga agatgctata
     3901 tgcaccatca ctgcggagca cgatagttca tttgacctac gttttgagag tgcccagtgg
     3961 gctgtaacac cagggcaatc agccgttctt tatcagggtg aacgctgcct tggcggcggt
     4021 attattgttc gctaagaact aagccgctgg tgggactgtt tgaacaaaag aagggcgctc
     4081 ggaaagccgt tcatagagcc ctcgtaaatt agggtattga gttttccagt ctaaataacc
     4141 aaagcgcaac tcaaaccagc caagtgcgca gccaacagaa atatcggcca aactaaattg
     4201 attgccatga caccacttgg catcaccaag accgccagcc atggtttcaa aaccgtgatg
     4261 tactttgtcc atttgtcgat caagccattt ggcactttgt tctgagggat tgcggaaggt
     4321 attctcaagg cgcaccaaaa ttccggcatc tactacacca tcggcgagag cttcccaggt
     4381 tttaacggca gcgcgctcgc gcccagtcgc aggaattagt ttgcccaccg ggcttagggt
     4441 atcgatgtac tcagcaatga cgcgggagtc aaacatagca ccaccatcat ccatgaccag
     4501 gcagggaact ttgcccaggg ggttgaagtc tgcaattgcc gttgatgcat cccagacatt
     4561 gtcaagcact agttcagcat ctattttctt ttcagccatc atgatccgta ctttgcggac
     4621 aaaggggcta gtgagcgagc caataatttt cataaggggc agtatagcaa tcaatcagtc
     4681 atttaagccg tgcggaaatc accaaaaatg cgtatcaagc cgtttgtcac taatgggttc
     4741 ataaacccat taaaatcagt attcaaccac tttgcaatga gtcgaccttg accaaactat
     4801 cccaactgag tgccctttcc cccctagatg ggcgatatgc caaaaagctg gatgccctac
     4861 gcccatggtt atctgaagca gcgtttatgc aacagcgggt tg
//
LOCUS       DDPQ01000086            9700 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5201,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000086 DDPQ01000000
VERSION     DDPQ01000086.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5201"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2475..2495
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3330..3363
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5251..5390
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5559..5652
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7288..7369
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8079..8117
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9186..9272
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9434..9575
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2388 a         2336 c         2108 g         2216 t
ORIGIN      
        1 agcgccaaac tagtgacctc agcctggacc tttactgaat tcgctagtgc attagcaatc
       61 aaagaacgta ctgatcaaat ttctcatcta caaagtaagg gtgcatggaa actctttgaa
      121 aaaatttgca ataatgatgt tgagcttttt tccatcaaac cgaatgtgtt ttactctgcc
      181 ggcttaatga ctttaaatgc agaatcgaat cttagagcgg gggatgcatt gcacctatcg
      241 gcggctattg acgctaaatc taaatccatt gtgacgctcg ataaagtctt agccaagaat
      301 tctcaaaaag ccaaaatcaa agcaatatta ctttaagtaa tagatcgaat cacttctaag
      361 ccgcccatat agggttgcag tgccttaggg atcttgatgc tgccatcagc ttgttggaag
      421 ttctccaaaa tggcgacgcc agttcttcct acggcaagac ctgaaccatt tagggtgtgc
      481 aagagctctg gtttattctg gcccacttta taacgggctt gcattcgtct agcttgaaag
      541 tcgcccatat tggagcagga gctaatctca cgataggcgt tttgcgatgg aatccaaacc
      601 tctaagtcgt aggtttttgt agacccaaag cccatgtcac cggtgcacaa gagcattctg
      661 cgataaggta gctcaagaag ctctaagatt ttctcggcat gtgcagtcaa ctcttctagt
      721 gcctgcatcg agttctcggg tttactgatg tgcactaact ccaccttatc aaactgatgt
      781 tgccggatca tcccgcgcac atcacgcccg taactacccg cctccgaacg aaagcatggg
      841 gtatgggcta catatttgag aggtagctga tcggcgctaa cgatctcatc gcgcacgagg
      901 ttcgtaaccg gtacttcggc cgtaggaatg agataaaagt tctcgaggcg ctcagcgcca
      961 tcatcgggat gctctcctcc catctttctg gggactttaa agagatccgc ctcaaacttt
     1021 gggagctgtc cggtaccgtg catcgaatcc gcattgacca tatacggtac atagacctct
     1081 tgatatccat gctgagaggt atgcacatcc aacataaact gtgcaagcgc acgatgtaat
     1141 ttggccgcat tgcctttgag caccacaaag cgcgcaccac taattttagt agccgagcta
     1201 aagtccaaac caatcggcgt acccaaatcc acatggtcct tgggtgtgaa ctcaaactgt
     1261 gttggcttac cccaagactt catctctttg ttctcatcag cagttttacc aacgggaaca
     1321 ctttcatcgg gcatgttggg gatccccaac agaaaatcct taatctcctc ttgcaatacg
     1381 ctcagtcggc tagcgcttgc ctcgagttct ttgttcacgg tggcagactc agctaactcg
     1441 gtcgtggcat cctcaccctt accttttttc ataccaattg ctttagccaa ttggttacgc
     1501 ttggcttgca actcttcggt acggccctgt aattgtttac gctcgctctc caattgcgta
     1561 aataaagagg tatcgagctc aaacttacga gttgctaggc gcgttttgac actctctaag
     1621 tctttacgta acagttgggg gtccagcatg gcgttcctta ttggtctaaa ccattagttt
     1681 agtcgcaaga cctctgcccc tggtggggcc ttaaactgaa aacggctagg ctcaatcttg
     1741 gggttgattt gcatctgacc aaagaccacc agaacaatgc tgccaaagcc atcggtcaac
     1801 tctaaggctt ttggcaaacc cccgcccatc ccgatcgcaa ttttggtgta aggcaaatca
     1861 ccctgtccta cttttgcctt aggcttgagg ttaacccact gtaaatcggt tttagtaccc
     1921 ccatcctcaa gctcaaattg cgaatctagg gcactctcac caaagaggat tgccgctggc
     1981 gatgctgcca aggcctgatt ggcagagcgt atcgtgagct gattgaggtc cttatcccac
     2041 atcaagagct gtttaccatc agcaattagt ttttgctcgt agggcttttg cgtgtcccaa
     2101 acaaacttac cgggtcgctg aaaaataaat tggccagagg tctgacggac taatttgggt
     2161 ttgtcttgtg gctcattcgg ttttggggaa cgcatctgct gctgcgcaaa ctcgccttgc
     2221 gcggattgca cggtctttac aaattggcgc aattgctcca cgccattagg gcggctatct
     2281 tgagagaagg tgaccgaact aaacgtgcct aggagtaaag caaggaaata atgaatgaac
     2341 ttcacagaaa agaatggatt ttgattaaac agatactact cagaaggacg cgagaccaaa
     2401 atctcacgat taccattgcc gcccatcttc gatacgagac ctgctttctc catatcttcg
     2461 agtaagcgtg cggcnnnnnn nnnnnnnnnn nnnnntggtc atagagggga tcggcttcac
     2521 cgctaccatt accgccacca ttaagcgcat ccatagtgga ctcgtcagca ccctctaaaa
     2581 caccgtcgat gtagtttgct tcgcccttct cctttaacca gctgaccaca cgatgaactt
     2641 cgtcatcgga gacaaaggca ccgtgcaccc gaatcggcaa accagtgcca ggtggcatgt
     2701 agagcatatc gcccatgccc aataaagcct cggcaccctg ctgatccaaa atcgtgcggc
     2761 tatcgacctt gctacttacc tgaaacgaga tgcgggttgg gatattggct ttgatcaatc
     2821 ctgtaatgac atcgacgctt gggcgttgcg ttgccaatat caaatggata ccggcagcgc
     2881 gcgctttttg agcaatccgt gcaatgagct cttcaatttt cttgcccgag accatcatga
     2941 ggtcggctaa ctcgtcgatg atcaccacaa tcaccggcgc tttataaagc ggctcaggat
     3001 cttcaggagt taggctaaat ggattagtga gtttttctcc tttggcttcg gcatccagaa
     3061 tcttcttatt aaagcccgcc aaattacgca cnnnnngcgt tataggcctg tttcatatcg
     3121 gtgactacgg gacacaataa atgcggcacc ttctcataga ccgacatctc cagcatcttc
     3181 ggatcaatca tgatgagacg cacttcatcg ggcttggctt taaagaggat cgacaaaatc
     3241 atggcattaa taccgactga cttaccggcg cccgttgtac cggcgactaa gcagtgcggc
     3301 atcttcgcca aatcagccac cgtagggctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnngagctca agatctccga gagataaacc gactgacggg ttggatttgg tagctccagt
     3421 gccatacagg ttttgccggg gatggtttct acaacacgca aactcaccac accgagtgag
     3481 cgtgataaat cgcgcgagag gttaacaatc tgactgcctt tcacaccaac tgctggctcg
     3541 atctcgtaac gcgtaaccac gggacctgga taagcggcaa tcactttggc ttgcacacca
     3601 aaatcatcga gcttgcgctc aattaaacgc gaggtgaact ccaaggtctc tgcagaaatg
     3661 gtttccttgg cttcggggac ggggtctaat aaagacaggg ggggcaactc agaatcgggg
     3721 atttctacaa agaggggttg ctgcttctcg cgctcaaccc gctcactctt cacgatcttc
     3781 agaggctgat taaagatttg tactttgggt gccacctctt cacgaatgcg cacctcttca
     3841 acgtgctctt ctcgctcgac ggcagcttgc tcaccgatct tgaggtcctc ttgcttctca
     3901 cgcttctcgc gcagacgccg aatccctaac tcgatatagc gacccacttg ctctgagagg
     3961 cttaaccacg agaaatgtaa aaagagggac aagcctacag ccaaaccaaa taacaaaatg
     4021 agtgtggatc ctgtaaagcc aaatgccatt tgtaatggat caccaatcac ttcgcccaaa
     4081 ataccaccgg ggggccttgg gagctcccac tggagggaat gcagacggat cgactcaagg
     4141 cccatgctac agactaaggt gagagcaaaa cctaaccacc gtgttaacag cgactctggt
     4201 ttgatattgg gatcaggagg aaggggaacg ctccacaact cgcgccattg gctaatgacg
     4261 cgtcgtgcaa acaaaatgac ccaccaaaaa gccgaggccc caaaaacata gaacatcaaa
     4321 tcggcggtcc aggcccccat ccgaccgcca atgttcttgg gggtctcaaa actagcatgc
     4381 gaccaagcgg ggtctgattt agtgtaagtc actaagactg caaagagtcc taggcaaatt
     4441 gccaatagga ggaaccagcg cacttcagca aataaacgag acatctttcc agttccgcta
     4501 tccggaggca atggggtcct agtttgtggg tacgagctac gtggcatgtt ctgatgattg
     4561 taaacaaggc taagccctag gaccctataa tttacccatg aataccacta ccaaacactc
     4621 caaagttcta attctgggct caggccctgc cggctatacc gcggcggtct atgctgcccg
     4681 ggcaaacctc aaacccacct taatcacagg tcttgctcaa ggcggtcaac taatgaccac
     4741 caccgatgtc gagaactggc ccgccgaccc caatggagtc caaggcccag agctcatgga
     4801 tcggttctta aagcatgccg agcatttcaa taccgagatt atttttgatc acattcatac
     4861 tgccgccctg accgagaagc caattcgtct ggttggtgac tcaggcactt atacctgcga
     4921 tgcgctgatt atttcgacgg gtgcctcggc acaataccta ggtctaccca gtgaagaagc
     4981 cttcatgggt cgcggagtct cgggctgtgc aacctgcgat ggttttttct ataaaggtca
     5041 agatgtgtgc gtggttggcg gcggcaatac tgcggttgaa gaagctctct acctcactgg
     5101 aattgctaag accgtgaccg tcatacatcg acgtgacaaa ttcagagcag agcccattct
     5161 gaatgaccgc ttgatggcca aagttgctga gggcaaagta atccttaagc tccactcaac
     5221 cctagatgag gtcttgggtg atgcaaaggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taacggttac
     5401 atcaaaacgc attccgggct tgagggcaat gcaaccgcca ccaacatccc tggtgtcttt
     5461 gctgctggtg atgtgcaaga ccatatctat cgtcaagcta ttactagcgc cggcacaggc
     5521 tgtatggcgg ctctcgatgc ccaacgctat ttagagggnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnatcagtgg gtttaccgct ggtccagctg tatctttgta gggatcgcca
     5701 acggtatctc cggtcactgc agctttgtga gcctcagacc ctttgccacc atggttacct
     5761 tcttcgatgt acttcttggc attatcccaa gcgccgccac cagtacacat cgagatcgct
     5821 acaaacaggc cagtaatgat ggtgcccatt aataagccac ctaaggctgc tggtcccaat
     5881 aagagtccta ccaaaattgg caccactact ggcaagagtg atggaatgat catctcttta
     5941 atagcagcgc ctgtgagcat atcaacggct ttgccatact ctggcttcgc agtgccctcc
     6001 atgattccgg ggatctctct aaattggcga cgcacttctt ccaccacggc gcctgcacag
     6061 cgaccaaccg cctccatcgc catcgcacca aacaagtaag gaattaaacc cccaataaag
     6121 aggccgatga tcaccatgtg attagagaga tcaaaggtaa cttgttgacc gattgcctct
     6181 aaggcatggg tatagtctgc aaagagcact aaggatgcaa gacctgctga accaattgca
     6241 taccctttgg tcactgcctt agtggtgtta cccactgcat ctaatggatc ggtgatatca
     6301 cgcacggagt ctggcaagcc tgccatctca gcaataccgc ctgcgttatc cgtaatcgga
     6361 ccgtatgcat cgagtgccac cacaataccg gctaatgaaa gcattgcggt tgcagcaatt
     6421 gcaatgccat acaaaccagc taagtaataa gctgcgtaga ttgcaataca tacaaagata
     6481 actgggtaag cggttgagcg catcgatacg cctaagccnn nnnnnntagt cggtaccggt
     6541 gtagtactcg gtgatccaga ccaaacctgc ggttaagagt aaaccaacaa tggaagagca
     6601 gaacaagcgc cactggcttc cttgaatgcc tagagcgtca tccggaacaa ttaagttggt
     6661 tgcaaagtaa aaaccaatta aagaaattac cccagaaata atcaatccct tatacagagc
     6721 gggcataacg tttttcatac ccgggcttgc cttgacaaac gagcagccaa tgatggaggc
     6781 aataatcgag atgccgccca atacaagtgg gtagaccact gcggctactg gcgcacccac
     6841 caccattaag gcacctaaga ccatggttgc aatcagagtc actgcatagg tctcaaagag
     6901 atccgctgcc ataccagcgc aatcaccgac gttatcgccg acgttatctg caatcactgc
     6961 aggattgcgc ggatcatcct caggaatacc agcctcaacc ttaccaacga ggtcagcacc
     7021 aacgtcagcc cctttggtga aaatgccgcc acctagacgc gcaaagatcg agattaaaga
     7081 ggagccaaac gctaagccaa tcaagggatg caaaatatcc gacaagtctt ggccgttatc
     7141 cagcgacact aacaacataa agaagagtcc aacacctagg aggccaagac caactaccaa
     7201 cataccggta atggcgccac ctttgaatgc cacattgagg gcctcgttca taccaaccgt
     7261 ggccgcttgc gctgttctga cattggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gataagcggc
     7381 cgccccttgc tgaatcgcct cagcgatctc catcattttg gcgttgccag tatcttgctt
     7441 gagaatccag gttcgcatga caaacccgta gatcactgct agtactccac agatcaatgc
     7501 aaagtacaac ccgtatgtga cattaatcat gctgcataac tcccttaatg aaattcatca
     7561 tgtattgcct atatttaggc taaatcatag tccagggctg aaagccttaa aataggactt
     7621 gtttagccct caaagtatcc aacagatttt gatctttcct gctttgctaa gatcagggta
     7681 attactgagc aggctggttt atttgattaa tttggagcat gtatgagtct tgacaacgtc
     7741 acccccggta aaaagatccc agaaaacttt aacgtcatca ttgaaatccc gatgaacgcc
     7801 gaccccatca aatacgaggt cgataaagaa actggggcga tctttgtgga ccgcttcatg
     7861 ggcacggcca tgcactatcc ctgtaactat ggctatatcc ccaaaaccat tgccggcgat
     7921 ggcgacccag tagatgtctt ggtgattacg ccctttccat taatccctgg agtggtggtg
     7981 agttgtcgcg cccttggcgt tttacaaatg caagacgaag cgggtgatga tgccaaactc
     8041 ttggccgttc cagaagatcg catcttatcg atctatagnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnttt gagcactaca aagatctaga aaagggtaag tgggtcaaga
     8161 ttaaaggttg gggatccatt gcggatgccc aaaaagaaat ccttgagggt attgatcgtt
     8221 atcagaaaga gaaaaaataa cgatgtgctc cagtagggtg ctgaggtaag caaggtggga
     8281 gatcgttctc caataaaaat cgctttggca caaatcaatc ctcttttggg ggattgcagt
     8341 gccaatgcag aactcatcta tcaagcagcc aaggatgctc atggtcaagg cgcatcccta
     8401 ctcctaagtc ctgagttgtc gctaaccggc tacccccccg aagacctact cttacgtcca
     8461 gccttcattg ccgatacaca aagagtcttg catcatttat gtgaacgttt agcagtgttt
     8521 cagggtttag cggtcattgt ggggcatccc agtcaagatg ctttgggatt gcacaatacg
     8581 gcttcagtat tacaagatgg tcaggtaatt gcgagctatc acaaacaggt cctacccaat
     8641 catgaggtgt tcgatgagaa gcgctacttt gtagctggta atgcaccgtg cgtgtttacc
     8701 caccagggct taaagctagg ggtcttacta tgtgaggata tttggcatgc agagcctgca
     8761 gcaaatacca agaaagctgg tgcagaagta ttactggttc taaatgcctc gccctatcac
     8821 cttgaaaaag acaatacccg tttagctgtt ctcaaagaac gcacccaagc cactggtttg
     8881 gcagttgtct atctcaatat ggtgggcggt caagatgaac tggtgtttga tggtggctca
     8941 tgcgccctaa acgcaaaggg tgagttggtt ttggccatgc ctcaatttaa agaagcgctt
     9001 gccgttctta caatccatga tggccaactc ttgctcggtg aaattactcc gcccctaacg
     9061 cttgaggctc aggtctactc ggccttggtt ttgggtacgc atgattacgt tcataaaaat
     9121 cgattcccgg gtgccatcat tggtctatca gggggcgtcg actctgcttt agtgctagcg
     9181 attgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacggcaga gatctcatgg atcgatgcgc
     9301 gcgagctcgc taaaaacttg ggggtgcaat acgacgagat cccaattagt caacccgttg
     9361 atgcaatcga aaatgttttg cgtaatcagt ttgccaatct caaacccgat gctacggaag
     9421 agaatatcca agcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatggc cgtaggctac tgcacccttt
     9601 atggtgatat ggccggtggc tttgcggtca ttaaagatat tgccaagact ctggtgtaca
     9661 aactctgtca ctaccgcaat caaatccaag agattattcc
//
LOCUS       DDPQ01000087            9522 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2146,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000087 DDPQ01000000
VERSION     DDPQ01000087.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2146"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1698..1737
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2710..2732
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4602..4611
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8208..8272
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2512 a         2231 c         2053 g         2580 t
ORIGIN      
        1 aagtcctaag aaaagtatga aattttgatt aaatccattt taccagcgcc agtattggct
       61 gaaataggcc gttgacccaa aattggaaaa ggggctagaa actagatggg ctatgacatt
      121 ttcccagtct gaccctgtaa atgcgttaag gcgtggtaaa aaaaatggcg cccgattgag
      181 gcgccattct taaaaaaccg aagtaaaact agctaaatta cacagcctta gcggtatgca
      241 aatgagcaat ttgctctttg gagtatccca aatcagtcag gacctcatcg gtgtgctcac
      301 ccaatactgg agatggctta acctctacct ttagctcaga gaacttgatt gggctaccaa
      361 tggttaggta cttaccgcgc accttgtgat cgacttccac gattgatccg ctttcgcgaa
      421 gatcgggaga ctccgcaagt tctttcatcg acaacactgg tgagcacgga atatcaaact
      481 tacgcaagat gtccactgcc tcatacttgg tcttatcttt taaccaatct tcaatggtgg
      541 cgaagatgtc aaaaatttta tcctgacgag cttgcgcagt catgtaggct ggatcggtcg
      601 cccactctgg tttgcccaat gccttggtaa ttggttccca agcatgtccc tgaatcgtga
      661 aataaatata ggcattagga tcagtctccc agcctttgca ctttaagacc cagcctggct
      721 gtccaccacc gccggcatta ccaccgcggg gtacgacatc actaaacttg ccatgaggat
      781 attgtggata ttcttcaagg taaccaacac ggtcaagacg ctgctgatca cgcaacttca
      841 cgcgacagag gttcaatacg gcatcttgca tcgagcaagc aaccttttgt cctttaccag
      901 tcttctgacg atgcatcaat gcggtcaaaa taccaatagc taagtgcata ccagtattgc
      961 tgtcgcccaa ggcagcggct gaaacggttg gagggccatc ccagaaacca gtagttgagg
     1021 cagcaccacc ggcgcactga gctacgttct cataaacctt gaggtcttca taagagtggc
     1081 catctgagaa gcctttcaca gaagccaaaa tcattttggg gttcagttcc atgatgcgct
     1141 cccatgagaa tcccatgcga tctaaggcgc ctggtccaaa gttctctacc atgacatccg
     1201 aaaccttgat caacttctcc agtacctctt taccctcagc tgttttggta tcaagtgtta
     1261 aagaacgctt attaccattg agcatggtga agtacaacgc atccgcgtct ggaatgtcgc
     1321 gcaattgact gcgtgttacg tcaccagatc ctgggcgctc aaccttaatt acatcggcac
     1381 cataaaagcc cagtaactga gtacatgctg gtccagcttg cacgtgggtg aaatcgataa
     1441 tacgaattcc gtctagtggt ttggtcatct tgaatctcct ttggactcta aatcaattaa
     1501 tcagattact tctttgttgc tgtcgatgcc ggatttagat tggttaaacg accgctctcg
     1561 gttccagcag tttcatcaat caccgcatta attaccgttg gcttaccaga agccagtgac
     1621 tggttcaatg catccatcag ctcttgtggg gtggtggcat taaagccaac cccgccaaag
     1681 gcttccatca tcttgtcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac
     1741 tgtgatgatc ggtaggttgt aacggcagat ggtttcaagc tccattccgc taaagccaaa
     1801 agcactatcg ccctcaatcg ctatcacagg ctgcttgctg acaactgcag caccaatggc
     1861 ataacccatg ccaataccca taatgcccca ggttccagag tcaaagcgct tgcgtggctt
     1921 atacatattg acgatgcttc tggcgtagtc caatgtattg gcaccttcgt tcacgatatt
     1981 tacatcggga ttggctttca caacatcccg aatgacgcgt aaggcactgt ggaagttcat
     2041 tggggatggg ttcttagaaa gagtctcctc catcttcgca atattcttat cgctcttttc
     2101 tttaattgcg ctaagccact ctgaacttgg cttaggaacg ctagctatct gcttcaaaag
     2161 ctcattaaca caggagccaa catctccaat caccggtgca gcgatcggta cgttgctatc
     2221 aatctcagtt ggtgcaatat caatctgaat aaatttcttc aaatggttgc cccaggtctt
     2281 acccttaccg tgagccaaga gccagttcaa acgggcaccg atcaacatga ccgtatctgc
     2341 ttctgacaat acgaatgagc gggctgcaga tgcgcattgt ggatgcgtat cgggcagcaa
     2401 gcctttggcc atcgacatgg gcaaatatgg aataccagac ttctcaacta aatctttgat
     2461 ctcctgatca gcttgagcat atgccgcgcc cttacctaga agaattaaag gacgcttggc
     2521 tgatttcaat acatcaatag cgcgctttac tgcgtctgct gcaggaattt gctttggggc
     2581 cgcatcaatg acgcggaaca aagactcttt acccttttga gcaggaatac tttgacctaa
     2641 aagctgggca ggaagatcca agtaaacgcc acctggacga ccagataccg ctgcacgaat
     2701 tgcacgcgcn nnnnnnnnnn nnnnnnnnnn nngcattgag ctgatccatc tcttcataat
     2761 cgccttgctg taaatccaca atctcgcgct cgctggagcc agagatcaaa atcatgggga
     2821 agcaatttgt ggtggcattg gcgagcgcgg ttaagccatt caaaaatcct ggggcagaaa
     2881 ccgttaagca gatgccaggc ttttgagtca tgtaaccagc gatcgctgct gcattaccgg
     2941 catgttgctc gtgacgaaat ccaataaatc gcattccctc tgcctgcgcc aagcgtgcaa
     3001 ggtctgtaat gggaatacca accaagccga agatggtgtc aatatcattt gctttcagag
     3061 catcgatcac cagatgaaaa ccatcagtta atggggcgtc ttctgccgta ctgtttgcag
     3121 aatgctcttt ggtcgctgtc aacataagct tgtctctctc attgggattg gtggtcttca
     3181 acattacctc cttaaaaaag ggtctcagtt gaactgagac gaatcttagg tctgcgcggg
     3241 tggatcagca ttgactttcg tcaatttccc ctaaaatcca ataaatactt agggttagct
     3301 cgtatttcat gggcctaaaa ctctgatgac ttatacaaaa agctctttat gcttactctt
     3361 aagcctactt agagtttctg gggaaagatt gagataggcc gctagttctt tcttgggcaa
     3421 aacctcaaaa aggttctcat acttgcgcaa gaagcgctca actcgaccag gggcgtccaa
     3481 catgtgaagc gtgatggtat gagccataat ttcgctcatt aagcgcataa cctcaaactc
     3541 aaaactctct tttaatttag ggtgagcatc caaaaactca gcccatttct tcagaggcat
     3601 acgcgcaacc cgtgctttgg taactgaggc aatactgtat ggcgctgacc gttgaaggcg
     3661 ccaagctgca taactcgttt caatatcttg ctcgattgca aaacgcagaa tcatctcttt
     3721 agcatcggcg cttgacacaa tccgtttcag aatcccgtcc aacacaaaat attgctccat
     3781 ctgatagctg ccctgacgca ataaaatctc agacttcttt agatcagaaa tctccaaaga
     3841 accctctaac tcagccatgc tgttttgatc taagctttgt aaaacaatgt tctggcttag
     3901 ctgcaagcgg atgagattct tttcgggatg cttatcgagt atggtcataa ttcctttacc
     3961 gtaacccctc attgtaagcc ttgaaaagta gcaaataaga gctttcctga gtcataaata
     4021 gggtaatatt gctgggtcgt ggtgcccgaa tgctttgcat tggggttaaa cgggaaacac
     4081 tatccaaacg tgtgctgccc ccgcaacggt aagtgaatgt gccaacacat cggcactaag
     4141 gtttgataag ccactgtgta gtcaacatgg gaaggctaaa ccccaaagtt caccagcccg
     4201 gataccggcc aagacaggtg gaatttcgcg gacggggacc ttcgcgcgcc gaggaaagcg
     4261 ctcgttctat acccgcgccc aattgacgct gatatcccta tccaagaatt tctgtatcgc
     4321 tcgctaacgg ggaggttggc aagcattaca acgtagggaa tcatcatgca atcatgcata
     4381 tcagtaaggc catctttaaa aaaggtggtg aagttatctg tctttgcttt actagcagca
     4441 actcttattc atacccaagc cgtaatggca caaagcagca gtgtctcagt agtaaatgcc
     4501 aacagctcaa tcgatcctgt cattgtcacc gcaacacgaa ctcctactcg ggctaatgat
     4561 gttcttgccg actatgtcta tattggtcct gaagaaattg cnnnnnnnnn natttcaacg
     4621 attggtgggt caggatcagc ggcaagcatc tttttaaggg gtgcaaattc caaccaagcg
     4681 ttacttctaa ttgacggtgt ccgctcacaa gcagctggca cgggtgcgtt ctcgttacag
     4741 gctattccac ttggcatcat tgaccggatt gaaatcatct ttggaccaca gagcagtttg
     4801 tatgggtcgg atgcgattgg tggtgtgatt caaattttta ctaaaaaagg aacaggagca
     4861 ttccaagnnn nnnnnggaga aagtaaaaaa actagctact ccttaagcag ctctcaggaa
     4921 atttcaactg gattcacaag tgttgcaaaa aataatgttt gcaacccaaa tacgcaatcc
     4981 tccaccacgc tttctaataa cttttgtgga agtggattaa atatgaatcg aactggatat
     5041 gtgagaactg gcggggctgg acgccttgcg caagagtgga ataaggggca ggagcttggc
     5101 cttcagtttg taactacgcg attagataac cagtatccag ctagcaatta ttttggtgga
     5161 ggaatcggaa gccaaattag taatttggga atattttcgt tgttttctaa taaccaaatc
     5221 aataaagatt ggaaaagtat gatccaagca tcccaatcca atgattactc tcaaaatatt
     5281 tataagacag gtaacccagt ctacaagacg aaacaaatga tctactcttg gcagaacgat
     5341 attaagattg gtaccgacct gcttcaaatt gttacagagc gtaaaacaat cagcgcatac
     5401 tccaatgatg acggagtaat tagtcaaaat caaaatacca atacgattgc gggatcatat
     5461 caattgaaac gagggtctca ccttggaaat gtagcgcttc gaaatgacag tattactgga
     5521 tatggcccac aaacgaccgg tagtgcctca tatggatatt tcttcacgaa tcaaattagg
     5581 ggcaacatta actatggcac aggatttaag gcgccatcgt ttaatgatct ttattttcct
     5641 aactacggta atcaaaattt acaagctgaa aaaagtaaaa atactgaaat tgggttgcac
     5701 tatgaatcaa aatcagttga tttacatatc attggattta atagcaccat aaccaaccta
     5761 attcaataca caactacatc gccaccttgt acctcatctc aactcaatgg tcctcagggg
     5821 ggttgtgctg gcaatgctgg tgaggccaaa atttctgggg tctcagttgg cggggttacg
     5881 agagtctcaa atcttagatt gcgaggctct atagatcaac aaaatcctat tgcccaaagt
     5941 acaggctatc ttttagcaaa acgcgctaga caattcggta acttaggtgc ggactataag
     6001 tataaaaaaa tgaatgttgg cgccgagggg acctttcaag gtggaagata taactctgcg
     6061 aattccggtt atatgggcgg ctatgcaatc tacaacctat acggcaatta tgagtttgcc
     6121 aaagactgga ccttgtttgg gcgttggaac aatatcttca acaaagatta ccaactttca
     6181 tatggttaca acaccccggg atccaatctt tttgtgggag tgcggtatgc tatgaaataa
     6241 tcatttttgg gctaatttag gtctaaaatg atcccttaag ttaaggagag tacatgttga
     6301 atttatcgac caaacaaact gttgtgatag cagtcgcttt ctccttggtc ttatgggcaa
     6361 ctcgggggca acatgttgcc tctttgtttg gtctgccaga tgctacttgg gctatctcct
     6421 tcatggtggg ctttttattt ggccctcttc tctttttagt aatcttggct caggcctttc
     6481 tgattgatta cttggcgatg ggtaattctt taatgaatat ggcttacttt tctctcatcc
     6541 ctgcctacct agctttgtac ttaactggta aatggtttcg gggtcagtat gacaagcaag
     6601 gtctgacatt gggtgctttt gcactgtcct ttattggcgg tattgcaatt tgtgagcttg
     6661 tttcaagtgg ctcattctat tttcagaaga gtacagcttc gttgagtggc tttgttgagc
     6721 tctttggtca gtatttcttt ggcaatttgt tctttgcctt ttgctatgtg ctggtttcct
     6781 cgctcgcctt ctatctaatg gttggcgcca cgaacaaaaa gctggcgtaa gcactaatca
     6841 accccatcca catggcgcac gattctttag aacaaatcct gcgtcgtctc tctgagaaga
     6901 tgaagatggt gaactcttct gttgaggctt tgcgccaaga tcgcatcatg ttagaaggca
     6961 agatcgaaga tgcccaaaaa cggattgaac atattctgaa gcgcttgccc gagcaaaacg
     7021 attctcgcca aatgaactta ttgactgata ccactccgcc tgtagataag gatcatgact
     7081 caacaacgca ttgaagtaag ccttgctggc cacaaaatca cccttgctac tactagcgat
     7141 catgaaccct tattgcgcga ggcatgcgcc tttgcggatg agcaaataca actagcgctt
     7201 gcgagtggca ataaaagtat tgagcgcgct gcgatgatgg ctgccattaa attagctggc
     7261 gagttaattc aggcgcagaa gatgattgct caatcctccc ctaatcctca ggttaatcct
     7321 gaacacctca ttcaaattga acaggaagtc cttgcacttg aacagcaagt agactccctc
     7381 atgaaaaccc tttccctgcc tggtgcgcca aggccaatag ttccttgaac cgatgcgtaa
     7441 gcatacggaa cgggcattgc agtatgggcg cgagcgtctg atcgatctac agtgcgggct
     7501 tgtcttgcac tccctgggtc atttgatgca cccgaagtat tgctaccgta ccaccttgaa
     7561 ctttagggtt caggatgacg gcctagcgcc caaggcgggg atcctttcat gcttctaacc
     7621 gatatcgcca tacttttggt ttgtggtgcc tgcgctggat tcttggcagg tctacttgga
     7681 attggtggag ggatgattct agtgcccttc atgatcatcg tgtttaacca ccaaaacttt
     7741 agccaagaga tcatcgtgca catggcgatt gctacaggca tgaccactat tttatttacc
     7801 tcgctttctg caatttgggc tcatcacaaa cacggctcga ttaactggcc attagtaacc
     7861 ggttttaccc ccggaattat tgctggtagt tttgtagggg ggagcgagct ctttgatgca
     7921 ttcaacacgg gatggctatc actctttttt gcagtcttta ttgtgtatac ctcgatccag
     7981 atgtttatca ataagaagcc aaagcctgag cgtgagctcc ctggcaaagt tggcttattc
     8041 tcatacggcg ctttttctgg agccctctct agtctgctag gagctggcgg tgcctttgtg
     8101 accgtaccat tcatgatctg gtgcaatgtg aaaccacata ttgcgatggc aacttcatcc
     8161 ggtttgggat ttcccattgc attggcatcg acgcttggct atgtattnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgtattgt
     8281 tgctaccagc atctttacgg cgccgcttgg ggcaaaacta gctcgcaagc taaatgtcgt
     8341 tcagttaaag cgtgtttttg gagtaatgct aatgttcttg gctttgttta tgttcaatga
     8401 agcccgcaag gcattgatta cctaatgact gtattgctca cgtagtatat ttttctgcac
     8461 tttgcccatt gcgttacgtg gcagactttc taaaatctct acccgctttg gcaccttaaa
     8521 gtttgcaatc tttaacttga gcgcatcaat cattgtctgg ccatttagtt tgatacctgg
     8581 cttgggaacc acaagggcca tcaccgcctc accaaaatct ttgtgcggta taccaatcac
     8641 agcactttct tcaacaccat ccatgtcatc gataaaactc tcgatttcct tggggtagac
     8701 gttataccca cccgagatga ttaaatcctt attgcggcca acgatgcaaa gataatgatt
     8761 aggcaccttc ccagcacttg tttctccacc ccagcgaccg acatcaccgg tcttaaacca
     8821 accatcgttc gtaaactctt cggcggtctt ctcgggcatg cgccagtagc ccttaaaaat
     8881 attggggcca cgtacttcaa tgccgccgat ttcatctgga ccacagagct ttccagagtc
     8941 gccaaccacg cgcacctcaa caccaggcaa cacaaccccg actgaaccac caatccgttc
     9001 cccctgatag ggattagaga ccagcatcac ggtctcactc atgccataac gctcaagaat
     9061 agtatgaccg cttacttttt ggaaattatt aaatgtttct gttagcaaag gagcggagcc
     9121 tgatacaaac agtcgcatat tcttacaaac ttctttattg aagtttggat ccattaagag
     9181 ccgaacatag aaagtcggga cgcccatcat taccgtagag cgtggcaagt gcttaataag
     9241 ctgtgcagta tctaatcgtg gcaaccaaat cattttgctg ccattgatta atgcaccatg
     9301 agcggctaca aagagaccat gtacatgaaa gataggtagg gcatgcaaaa gtacatctcc
     9361 ctttttccat ccccagaact tgtttaagac cagggcattg ctacctaagt taccatgggt
     9421 caacatggcg cctttacttc ggcctgttgt acccgatgta tacaaaatag ctgctagctc
     9481 atcatcggcc ttagtaaccg tcttaaattg atctgcacaa tg
//
LOCUS       DDPQ01000088            7879 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5633,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000088 DDPQ01000000
VERSION     DDPQ01000088.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5633"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2335..2393
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2092 a         1937 c         1851 g         1930 t
ORIGIN      
        1 ttttaatagg atgcgttagc ctcaatgata accgctctag attacgaaat tgtttatcaa
       61 aagcactaaa ctagagccat gaactcccac gatctgctta cccaatatgg cccgcgcgaa
      121 gccatggaat acgatctggt cattgtgggt ggaggccccg ccggtctatc ggctgccatt
      181 cgggctaaac aattagcaca gcatgagggc cgagagctta gcgtctgcgt cctcgagaaa
      241 ggttctgagg tcggggctca tattttatct ggggccatca tggatcccag agcgctcgat
      301 gagctcatcc ccaattggca agaactaggc gcccctctcg aaacaccggt tagcaaggat
      361 cagttcactt tcttatcaga gactggttct ttcacagttc cccatctact tttacctcgc
      421 tgttttagca atcaaggtaa ttacatcctt agcttaagca atttcacgcg ctggttagga
      481 gtgcaagcag aaaatctcgg ggttgagatc ttcccaggct ttgccgccgc agagattttg
      541 tataacgcgc aaggcgctgt ttgtggtgta gcaactggta atctaggtgt gagcaaagat
      601 ggtgagccaa cagaacaatt tcagcttggt atggagttgc gcgccaagta caccctcttt
      661 gctgagggat cacgcggtca tcttggcaaa caactcatta gtcagttcca attagaccgg
      721 agtgccgatc cccaaagtta tggcattggt attaaagaac tttgggaaat tgctcctgac
      781 aaacatcagc caggcttggt tattcatact gcaggatggc ccttagacag caagacctat
      841 ggcggctcgt tcttgtatca ctttggtgaa aacaaggtag cggtcggtat ggtgattggt
      901 ctttcttatc gcaatcccta tttgagcccc tttgaggagt tccagcgtta taaattgcat
      961 cctgcgattc gttcgacctt tgagggtggt aagcgtattt cttatggtgc acgtgcaatt
     1021 actgcgggcg gtttaaatag tttgccccaa accgtttttc cgggtggcgc tctcattggt
     1081 tgcgatgcag gatatctcaa tgcctcacga atcaaaggta gtcatgccgc aattaaatcc
     1141 ggcatgctgg ctgccgaggc agtgttatct gctctctcag aaaatcgagc taacgatatt
     1201 ctgaccgctt accccagtgc atttaagaac agttggcttt accaagaact aaagcaagcc
     1261 cgcaacttca aaccgtggat gtcaaagggt ttgtatctgg gtacgctgat ggtcggtgtt
     1321 gagcaaaaac tattgggcgg taatgtgcca tggacgattc atcaacgcaa ggctgattat
     1381 gaatatcttg agcctgcaaa actgcatcaa gtgattgatt acccaaaacc ggatggcacg
     1441 attacgtttg atcgtctttc ttcggtgttc atctccaatg ccaatcatga agagaatcaa
     1501 ccctcgcacc tcaccctgaa agatcaggat gtgccggtcg ccattaactt agatgtgtat
     1561 gcaggtcccg agcaacgtta ctgccctgct ggtgtttatg aatatgttga attcgaaggc
     1621 agaccaaagc tacaaatcaa tgcccaaaac tgtgtgcatt gcaaaacctg tgatatcaaa
     1681 gatccaactc agaacattgt ctggattacg cccgaaggtg gtggtggccc caactactcg
     1741 gggatgtaat taagtcttac tcggactaga ctggacttga taaatcgcta gccagccgag
     1801 tgcgcaagca agtgcggcca tcccaaaaac ctgattgggt cctagttgat cccaaatcca
     1861 gccggcacat aatcccccca aggtaccccc aaatccatac gctatggtgg taaataaagc
     1921 ttgaccacgt gcctgcatcg gtcctgcaaa ccaggtctgt attaatcgag tactagcgct
     1981 gtgatgcgct gcaaagctgg ctgcatgcat gagctgtgcc aggattaaaa gactggtact
     2041 tgcaaaataa ccaatcaaca caaaccggat cacaccaaat aaaaaggtca cctgcaatat
     2101 ggctgtcggt gagaagcggg ccatcacctt actctgtaaa taaaagaaga ccacttcagc
     2161 aaatacaccc agagtccaaa agagaccaat ctcaaacttg ttgtatccaa ggtctgcaag
     2221 ataaagtgaa taaaacacat acaaggcagc gtgtccgaag atcatcgcaa aggcagatag
     2281 caagaaccag cgcacttcgg gcctgcgaag gaccgaacgt aactcaccac gcacnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccaagga
     2401 aaaatcacaa tgccttgcca ctgaaaaatc tctccagcaa ttaaaaccat ggtgataaat
     2461 ccaattgatc cccataagcg tatgcgccca tagcgatgat agaaagactc gtctttgtat
     2521 aaagcatgta cggtcgccgc ctcacccaat ggcaccatac tgctcaaaat agtgtgtaag
     2581 acaaacatcc aaattaatag agggatgtat tcctgcaaat aaaaaattgc tacaaaggta
     2641 atgcaggcaa ggactgcaca aaaacgcatg atcccaacgc gatttgataa gtaatccgag
     2701 agccagcccc atgaaaatgg tcccaaaatt cgagttacct gcaacatcga cattaaggct
     2761 gcgatctcga tagcaccaaa gccgagctct gcaaagtaca agcttgcata gggcgacatc
     2821 aaaccaatat aggcgaagta caaaaagaaa aaggccccaa aggcccagcg ggaggaaact
     2881 gtcattctca gtaaattaac gagtgcgggt cgctgggatc gcgctagagc ttactttgac
     2941 atcggcacat tgcgctcgat gacgcaaggc gtgatccatc aatactaagg ccaacatcgc
     3001 ttctgcgatg ggtgccgcac ggatacctac gcaaggatcg tgacgacctt tagtttgaat
     3061 gacacttgat ttgccatcaa tatcaatagt ctctttgggg ctcatgatgc tcgaagtagg
     3121 tttaatcgca atcgagacgc gtagatcttg cccagtgctg atacccccga gtgttccacc
     3181 agagttattg ctcgcaaaac catctgggtg catctcatca ccgtgctcac ttcccttttg
     3241 ggcaactacc gcaaaaccag cgccgatctc aacacccttc acggcattga tccccatcat
     3301 cacatgggcg atgtctgcat ccaatttatc aaacagtggt tcgcctaggc caatcggtac
     3361 tttactggcg cgcacctcaa tctttgcccc gcaggagtct cctgctttac gcaactgatc
     3421 catgtactcc tcaagttgag gaatgattgt ttggtttgcc gcaaagaatg gattttgcgg
     3481 aatctgtttg gcatcttcaa acgggatcgc tatctcacca agctgactca tatgcgccac
     3541 aatctcggtg ccatgctgct cgcgcaacca tttttttgca atcgctgctg ctgcaacaac
     3601 cggtgccgtt aggcgcgccg atgaacgacc gcctccgcgt ggatcccgaa agccatattt
     3661 atggtgatag gtgtaatccg catgacccgg acggaaggtc tgcatgatgt cgccatagtc
     3721 ttgactgcgc tgatcggtat tacgaatcag taagcaaatg ggcgtaccag tagttttacc
     3781 ttcaaagacc cccgataaga tctcaacccg atcttcttct ttacgttggg tcacatgacg
     3841 agaggtacca ggcttgcgac gatccagatc accttggata tctgcttcag aaagggtcat
     3901 accgggcggg cacccatcca ccactgcccc aatggcagga ccgtgagact caccaaaagt
     3961 ggtgacagtg aacagaaggc ctaaggtatt tccggacata gctccattat ggcactaggc
     4021 ggatcaatgc tcctgaacca agcgttgccg aatagttggc atattggcca cgatcaccgc
     4081 gcccgctaag gttacccacc aggtgatata aatccagatc agcatcagag gcacaatcgc
     4141 aaacgtccca tataccgttt tgtagaaagc agcttgggtg acaaaaaagg caaagccaaa
     4201 ttttgtgagc tcataggcga ggcctgcaaa catagccccg gtcaatgcgt ctctccactc
     4261 aactttcgta aacggcaaga cacggtaggc cagagcaaaa acaatgaaag ccaaaagact
     4321 cgatacgatc gtgctcatga acttaaagcc tgctgaaagt gctgggaacc agccactcgc
     4381 tgatgcaaac aagacgccac ttagatatat accaaaaccc agtaagagcg gtcccattac
     4441 cgtagcagca agataaatta gcatcttctt cagaaatggt cggcgctctt tcacttgcca
     4501 gatctcatta aaggcacgct caatgacgat catggtcacg atggtcgtga gcaataagcc
     4561 aacagatcca accagagtta actctttagc ctttgtagaa aactggctga gatagtttga
     4621 tatttgctga ttaatgccac ccgggaaata gtttttcagt acccaggtct gaaaagtggc
     4681 tcgtaacgcc accacgcccg gtaagttcga taccaaatag gatccgatgg taaggattgg
     4741 caccagcgag aggatggtgg taaatgccaa gctggcagat acttgattga gctttaggtg
     4801 ccgattacgc tcccacaact ctttaccgag aggaagccaa cgctggattg aatacgttaa
     4861 ttgcatgccc gtatcatatg ggaatctata ctgaattcca aaggaagcgt ttcatatgaa
     4921 ctccccagaa atcttggtct tgtattactc ccgctctggg gctacccaga aattagcgcg
     4981 cttaatagcc gaaggtattg agagtgtctc gggagtcagc gctcgtttgc gtaccgtgcc
     5041 agccatctca gcggtctgtg aggctacaga acccacagta cccaccgaag gggcacccta
     5101 tgcggaatac tctgatctag aagaatgtat tggtcttgct atgggctcac ccactcgctt
     5161 tggcaatatg gccgcgcccc tgaaatactt tttagatggc agctcggctc aatggttgag
     5221 tggtgcgctc caaggtaaac cggcgtgcgt ctttacgagc accggtagct tgcatggcgg
     5281 gcaagaaacc actctacttt cgatgatgct ccccttgatg caccatggca tggttttgtt
     5341 ggggctaccc tatagtaatt ctgagctcat gaatacaaca acgggtggca ccccgtatgg
     5401 tgtttcacat ctagcgcatg cagacggtag tgccccgatt agtccagaag aaagtcggtt
     5461 agccattgcg caaggaaagc gtcttgcaga gattgcgcta cgcctgcgag caacaaactg
     5521 atgggtaata tgctctccaa aatccttgat aaggataaat acgttttaat tgcgagtgct
     5581 gcgacgctag accttatcct tttgtgcatc gcttgggaat ggtttatctc tccgctacgt
     5641 ccgggcggct cttggctgat tctgaaagcc ctgccactaa tcatcctctt gcccggccta
     5701 tggcgagcca aggtctacac tatgcaatgg ggtacgatgc tcattctagt ttacaccacg
     5761 gagggtttag ttcggctctc cgaatcgggt tggaactttt ggatggcact attagagcta
     5821 gtgttctcgg tcatcgtctt tgtatgcctg ctcttgtatt taaagcccat caaggcagaa
     5881 gcaaaggcaa aggcgaaagc ggatcccaaa gcaaacacta cccccaagca atagaatgat
     5941 ggcatgagcc cttctgcatt catttcacat tgccaaaaac ttctggggca gccatatgtt
     6001 ctgcttgacg atcaggacaa agcgccctac ttaagcgatt ggcgtaatcg ttaccaaggc
     6061 aaggctcttg ccatcctttt gcccaatacc actgcacaag tggctgagat tgtgaaggca
     6121 tgtaatcagg ccaagatctc tatcgtgccn nnnnnnngtt atgaacctca agcgcatgca
     6181 ggccatacgc ggaattgatg cgagcaatca aacgatcgtg gttgaggctg gctgtatttt
     6241 gcaaactgtt caggaagcag ctaaagcaca aggattttta tttcctctga gcttaggcgc
     6301 gcaaggtagc tgtcagattg gtggtaattt agcaaccaat gcgggtggca ccaatgtctt
     6361 gcgctacggc aatgcgcgtg agctgtgttt aggtcttgag gtggtcactg cacagggcga
     6421 ggtcatgcag ggtcttcgca gtttgcgaaa agacaacacc ggttttgact tacgcgatct
     6481 ctatattgga tccgagggtt gcttgggcat catcactgcg gcggtgttta agctatatcc
     6541 gcttccaaca gcgcagtgga cagccttggt ggcagtgcct gatattccat cgaccatcca
     6601 gctcctcaac ctctttcaga aacgtacggc tgctttactc accggctttg agatgatgtc
     6661 taaagattct ttagacctga ctgcaaaaca ctttccgcag atgaagaacc ctctgtccaa
     6721 cgatccagcg tataccgttt tggtcgagtt atccgatcac gaaagtgaag agcacgctcg
     6781 caatctaatg gagagtgttc tagaaagtgc ctttgagagc ggtctagcga ctgatgctgt
     6841 gattgctagc aacctctctc aggctcaatc attttggacc atgcgcgagc atattacgat
     6901 ggcccaagcg caagaaggcg ctaatctcaa gcacgatatc tctttaccca tctcttccct
     6961 tcatgctttt attgaggaaa ccgatggtat cttgcgacaa cgcttcagtg gcgtgcgcct
     7021 tattaacttc ggacatcttg gggatggtaa tttgcattac aacgttgctc cccccgttgg
     7081 aacaaacccc aaagaattta acagtagtca tgagcaagta attcatgatg tggtctatac
     7141 gcaagttgag aaacaccatg gctctatctc tgctgaacat ggtattggcc agctcaaact
     7201 acctgattta gaggctcatc gaggctctgt agcccttcaa ctgatgcgta gtctgaaaca
     7261 tgctctcgat cctaacaaca tcctaaaccc tggcaaagtg atctctcaat aatgcttcgc
     7321 ttacttttta tcctggcgct atttnnatgc gccatgctga tgccccagga tttagtgacc
     7381 cgcaaggtta tcgtctcaat gattgctcta gccaacgtaa tctcggtgat tacgggcgca
     7441 aacaagcatc agagattggt cagtggcttg cagcccaagg aattaaagaa gcccgggtct
     7501 ttagtagtcc ttggtgtcgc tgtatggata cggctaccct ccttaataaa gggaaggctg
     7561 ttccagagtc ttcgttagga tctttcttcg aagaaatgag tcggggggac aaacaaacaa
     7621 gagagcttga ggcttttata caaaaacaat tatcaagcag caataaggcc ccgttgatct
     7681 tggttagtca tcaggttaat atccgcgcct tgactggtgt ctcggtcggc tcaggacaaa
     7741 tgctcttggt gaaggtgaac aaacagggtc aggtgatcca acaacgttct attccctgat
     7801 cgttagagct tgatgagatt caatttgact aaggctaaaa atgagaaaat gcgcttatga
     7861 gcaccattat tgacccagc
//
LOCUS       DDPQ01000089            4200 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_24099,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000089 DDPQ01000000
VERSION     DDPQ01000089.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_24099"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2153..2171
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4012..4066
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1015 a         1092 c          929 g         1088 t
ORIGIN      
        1 gcaagcaatt gatattggtt tgaacccatg tgctctgcta tatgtgcggt cttaccacca
       61 ggcgccgcac acgcatccaa tatatgatcc cccgcctgtg ggttcaacaa tagagtcgcg
      121 agctgggctc ccgcatcttg aacagaaacg agcccctcat caaatccggg tatctgcgat
      181 accggaatcg gctcttgaat cactatcgca gtatcaagtg ctatgccact caaatgctca
      241 atgcgtctgt atgaaatttc ttttnnacta gagggggtct cttgccttgc tctatacaaa
      301 tgccctgcca ttcttttgga taagcggctt gtaattgctc aagccaccat cttgggaaag
      361 caataccaaa cccttggccg acaagatctt catctttgct tgaaacagct attttgcgca
      421 agaccgcatt tatcagtcct tgcgcaaact gggtctttgg cgagctacct gccgccttca
      481 ctgcctcgtt cactattgtg tgtgccggat aacgattttg catgtctccg cgctcttggc
      541 caagaataag agtacacgac acgctcaaga gtagatcaac ttctttgctg ggttttttgg
      601 ggaccagttt ctgaatccgt ttttgcgcat gcgcccaaga acgtaaggcc gcaaaggtaa
      661 cagactgtat cgctggtcgc aatttaggat cgagggtgtc aagaacctgg cttaatgagc
      721 gcccctcata aataatttgc tcgatggcgt tagccgagcc aagcagtacc tgggcaagcg
      781 cggcaccgtg ggttgccgcg cctacaccct ttatccgttt atccaggata acttcctgtt
      841 cgcgccattt ccattaagcg cgccacacga tctgccgttg cgggatgagt tgaaaaaagc
      901 tgcgatagtc cggaagctgt cagtggattc atgatcatca tttgcgccgt ttctggatga
      961 tgctcaaccg catcaaatgg tatgccgctc gcatagcgat gtattttgtc aagcgccgag
     1021 gcaagggcct caggatcttg tgcgatctct gcgccccccc gatcagcttc atactcgcgc
     1081 gcgcgcgaga tactcatctg aattaagcct gccgccattg gcgcaagaat tgcgaccaag
     1141 atacccgcaa ttggattccc agaacgtccg tccgagcctc ggctaccaaa caacatagcc
     1201 atattggcaa gcgcagagat tgcaccggcc atcgttgctg caatcgtaga gattaaaatg
     1261 tcgcggtgct tcacgtgggc taattcgtgg gccatcacgc cacgcaactc gcgattcgat
     1321 aagatgcgga gaattccagt ggttgctgct acggctgcat gctccggatt gcgccccgtt
     1381 gcaaaggcat ttggtgagtc ttcattaatt aagtaaacct tgggcatcgg cagctccgct
     1441 ttggctgcca actccttcac aagctcataa aactgcggtg cggagttctg gtctacctct
     1501 tgtgcattag tcatccgcaa gaccatcttg tctgagaacc aataactaaa gaagttcata
     1561 gctagagcaa acaacaaagc caagaccatg ccttcatgac cgcccaacat tccaccaatc
     1621 acaataaaca gggctgtgat cgccgccatc aaaattgcgg ttttcataag attaaacata
     1681 tatccccttt tttctctatt gatgaaggtg agcacagccc atcatctata ttcctaatcg
     1741 ctcatcttat gggattcact caaaaactgc ttaagcgggt ttgtagcatt agctaaaaac
     1801 catgttcgcg ctggtatctt tttaccaccg ggcttttgca gctctaggat ctcaagacac
     1861 tgatctttgc actgaaccca aaaaccttcg gcgctttggc caacaataga tcctggtagc
     1921 ttctgtgctc caaaggcatc ttgctgctgg ggtttgggta agcgaaccct ccaaatctta
     1981 atcaactctc cgtcaagttg tgtgcttgcc accggaaaag gattaaaggc gcgcaccttg
     2041 cgatcaatct cttgggctgt tttatgccaa tcaattaaag cctctgcctt caaaatctta
     2101 ttggcataac taagtccttc cttgctttgt ggcaaaagat gaatgtcgcc tgnnnnnnnn
     2161 nnnnnnnnnn ncagggccga tctcaagctc tttactaatt aatactggcc cggtatcaag
     2221 tcctgcatcc atcttcataa tagagacacc cgttttggta tcacctgctt cgatggctcg
     2281 ctgaatgggc gccgctccac gccaacgcgg cagtaaagag gcatgaatat ttaagcaggc
     2341 aaagcgccca ttgcgttcag ctgcttgtaa aacctcgcta ggcaaaatca gtccatatgc
     2401 aaccactacc atcacatcaa actgtatttg catcagctgc gcaagcgttt cttttgcatc
     2461 ctgctgcttt tggggatcgt gcgcatgaat gttcaagctg agaggctgcc aaactggaat
     2521 gtttttttct tgggcaagca gcttgacggg gcttgcttgg aggtgcatgc cccgacctgc
     2581 tggccgatcg ggctgagtga gcactgccac aatttcgtgc cctgcatcca ttagtacgcg
     2641 caatgcctca gctgcaaaat cgggagtacc cgcaaaaatg attttcattt gtaatggtct
     2701 gcgtttaacg aagcccaatc aattcttttg ctcgcttctt gagttttaat gaaatgcgac
     2761 tccgctttaa tggggaaaga tattcgacaa aaaccttacc ttgcaaatga tccatctcat
     2821 gctgcaagca aaccgccaaa agtccgtcag cctcgagttc aaagaattgg ccctcttgat
     2881 taagtgactt gatcttaatt cgttcgggac gctcaacttc atcaaagaaa tccggcaccg
     2941 aaaggcagcc ctcacgccac gatttttttt ctgggcttgc ccaaattagt tcggggttaa
     3001 taaaaaccat cagagcatcc cgctcttccg aggtgtcgat cacgatgatg cgctcatgaa
     3061 tatcaacttg cgtagcggcc aaaccaatac cggaagcctc atacattgtc tcggccatat
     3121 cagcaacaat ttgctgaata cgtgcatcta ctgacgccac cggttttgca atcgtatgta
     3181 ggcgctggtc tgggtaatta agaatcgata agacagccat attccaatta tcttacaaac
     3241 aaatcgggtt gaccttcttg gcagaggccg aggggtaagc aaaaataatc tcatgagcaa
     3301 atccaccacc ctctcttctg aagacttctt cccctcgatc tgttgcctaa gccaggattc
     3361 ggccgactat ccgaagcgtc tttgtgatct gagcgatccc ccacgcaaac tattttatat
     3421 aggcaatatt gccctattaa ggcagcccat gttagccatt gtcggcgccc gaagggcctc
     3481 cagggccggg ttactatatg cccaacattt tgcagccggt cttgggcttg cgggttatca
     3541 catcgtctct ggtcttgcct acggtatcga cagcgccgcc caccgcagca tattagattt
     3601 agatggcccg agcaaaacga ttgcggtttg tggcaacggt cttgaccgtg tctacccatc
     3661 cgagcatcaa gccctagcaa aagaaattgc aaaagaaggg ctattaattt cagaataccc
     3721 accgggtatc gggcccaaag gctttcattt tccaaggcgc aatcgcatta ttgcggccct
     3781 agcactcggc acagttgtga ttgaggctgc tcaaagatcg ggctctttaa ttaccgccta
     3841 ccttagctca cacctaggcc gagaggtctt tgtcgttccc ggccccattt cctcgcccct
     3901 ttacgccggc tcccatcgac tgattcaaga tggagcgaaa ttaattagcg gggttgagga
     3961 cgtccttagc gagctgccca gtgcccgttt tcattaaaag cgggtttatg annnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttta ccgattatgc
     4081 ttctgattac tatttgactt taatttaggc tttgggctaa taataaaaaa ggggcggctc
     4141 tggctttctc taaaaaaacg tttaaattag gcttcctttt gacccaaaaa cgagatccaa
//
LOCUS       DDPQ01000090           16317 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2361,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000090 DDPQ01000000
VERSION     DDPQ01000090.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 16317)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16317)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16317
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2361"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2344..2450
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5321..5356
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7512..7613
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4382 a         4113 c         3494 g         4083 t
ORIGIN      
        1 aatgctttac gcatcgcatt ttttgtctct tccgttacta tcttgggacc cgttacgtac
       61 tcaccgtact cggcattatt cgagatcgag taattcatat tggcgatacc gccttcgtaa
      121 atcagatcaa caattaactt aagctcatgc aaacactcaa agtacgccat ttctggtgcg
      181 tagcctgctt ctactaaggt ctcgtatcct gctttgatta actcaacggt accgccacaa
      241 agcactgcct gctcaccgaa caaatctgtt tcggtttctt cgcggaaatt agtttcaatc
      301 acgccagcac gaccaccacc attagcagtt gcataggaaa gtgctaaatc acgtgctgca
      361 cccgatttat cttgatatac agcgatgaga tgcggaacac cgccgccttg ggtgtaggtg
      421 ctgcgtacag tatggcctgg agcctttgga gcaatcatga tgacatccaa atcagcccgt
      481 ggcaccactt gtccataatg cacattaaag ccatgagcaa atgctagggc tgcgccctgc
      541 ttcatattgc catgcacttc actcttatag acttcaccga tttgctcatc tggtagaagc
      601 atcatgacca catcggcatc cttcacagcc tcagcaacct ctttcacctg caagccagca
      661 ttagctgctt ttttccatga ggcgccgtct ttacgtaaac caaccgtcac cttcacgcct
      721 gagtcgttca agttctgtgc atgcgcatga ccctgtgaac cgtaaccaat gatcgttact
      781 tttttacctt tgatcaggga cagatcggcg tccttgtcat aaaaaacttt catacttttt
      841 ccttaaatta aacgaagaat aattgaaatt aaaccttgag aatccgttca ccgcgaccaa
      901 tacctgatcc acctgaacgc actgtctcaa gaatggatgc acgatcaatt gcgctgataa
      961 atgcatcgag cttactactc acaccagtca gctcaatggt gtaactttta tcggtcacat
     1021 caataatgcg tcctctaaaa atatcggcgg tgcgcttgag ctcttctcgc tctttaccaa
     1081 ctgcacgcac tttaatcatc atgagctcgc gctctaaatg cgctccctca gtcaaatcaa
     1141 aaaccttaac aacctcaacc aagcgattta gatgttttgt gatttgttca atcacatcat
     1201 cagagccaat ggtcacaatc gtcatacgcg atagtgatgg atcttcagta ggtgcaacgc
     1261 ttaaggtctc aatgttgtaa ccgcgcgctg agaaaagacc aaccacccgt gataaagcgc
     1321 caggttcatt ctccagcaat acagaaataa tgtggcgcat tacaaatcct cacttccaag
     1381 caacatctca ctaatgccct tgccagcttg caccattggc caaacgtttt cctctggatc
     1441 gatctggaaa tccataaata cagttctatc cttgagcttc agagcctctt taagtgctgg
     1501 ctcaacatca gccttggtct caatccgcat accgacatgc ccataagcct ccgcaagctt
     1561 cacaaagtct ggtaaagaat ccatatatga gctggaataa cgtttgctat aggtaagttc
     1621 ctgccattga cgaaccatac ctaagtaacg attattcaag gacacaatct taattggcgt
     1681 gttgtattgc gtgagcgtag agagttcttg aatacacatt tgtatggaac cctcacccgt
     1741 gatggtgacc acctctttat cagggaaggc tttcttaatc cccatggcat agggcaagcc
     1801 aacacccatc gtgccaagac cacctgagtt aatccagcga cgaggttcat caaacttata
     1861 gaactgggca gcccacattt ggtgctgacc tacatcggag caaacatacg catcgccttt
     1921 ggttaactcc caaagttttt gaaccacata ctgcggcttc acaatttgtg actcacgatc
     1981 gtacttaaga caatcttttg tttgccactg tgcaatctga tcccaccagg ctttaacctt
     2041 cgcttgattc tttcgggcag gtgcactccg aatctgtgcg gacatatctg ccaagatctc
     2101 tttgaggtcg ccgacgattg gcacatcgac cttgacgcgc ttagagatca cagacggatc
     2161 aatatcaata tgaataattt tgcgtggatg gcttgcaaag tgcgctgggt taccaattac
     2221 acgatcatca aaacgcgcgc caattgcaat caatacatcg cagtgctgca tagccatatt
     2281 ggcctcgtag gtaccatgca ttccgagcat gccaacaaac tgctggtgag tacctgggaa
     2341 tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccaccggtat
     2461 aaatgtacgg gcgttccgcc tcttgcagca gagaaacagc tttccgaatt tgtccgctat
     2521 gacccttaac cactgggttg taggaacgca tattgagagt gtctggataa acaaacgcgc
     2581 ccttagttgc cgatacatcc tttggaatat ccactaagac cgggccaggt cggccagtgc
     2641 gcgcaatatg aaatgccttc tttaaggtca aagcaagatc tttaacgtct tttactagaa
     2701 agttatgttt tacgatcgga cgcgtaatac caacggtatc cgcttcttgg aaagcatcct
     2761 caccaatagc ataggttggc acgttgccgg taatgatcac catgggaatc gaatcggtat
     2821 atgcagtagc aataccagta accgcattgg tcactccagg acctgaggtt acaagtgcaa
     2881 caccaacctt accggttgca cgggcataac catcagcagc atgcacagca gcttgctcat
     2941 ggcgtacaag aatatgttca aacttatctt gcttgaatat ctcgtcataa ataaagagaa
     3001 ctgctccgcc aggatagccc cagacgtatt ccacgccttc agcatggaga gcatgaacca
     3061 gcatctctgc accaatcatt tctggaccag agggtggcgt attattttct gaaattaccc
     3121 cacctggggc ttgactagct ttgctagcta aaaattcagc gcttgttgta ttcatttttc
     3181 cttttccttt gcaattttcg gcaaaaaatt gtttaggctg ttctgtccca tacttatggt
     3241 ccgggttcga acggcaccgc cgcgcagaaa attaaagcca cttcttggat ggtttctttg
     3301 cggtaagcct tatattgtaa agcaatagcc tagaatgagc gagctaagcc cttaattcag
     3361 atcgatttat aagctaaatt gtcccccttt aacctatcct gcaagcccca gatttagaat
     3421 ggcatccgcc caagaactga accttttcct agccagtatt gagaaaaagg cctttaaaca
     3481 ggctgtttat gccgttcggg atgatgccag cgccctagac attgttcagg atgccatgat
     3541 tagtctggct gaaaagtatg ccgatcgccc tgctagcgaa cttcctttga tcttcacccg
     3601 aattctccaa aatcggattc atgactggtt tagacgccaa aaggtccgca atacctatga
     3661 aacctctttc tcctccctgg gctctggtca agatggcgag gatagctttg accctctaga
     3721 actgcttgaa atcagggatg acgcccaaat atcccaagac ggggaggaaa agctcgcaca
     3781 aagtcagtta ttggctatcc tagaggctga aataacaaaa ttgccaattc gtcaacgaga
     3841 ggccttcctc atgcgttatt gggatgagct aagtacggcc gaaacagctc tcgccatggg
     3901 gtgcagcgag ggaagcgtta aaacccattg ttcccgggcc acccatacct tggccgctgc
     3961 cttaaaagcc aaaggaattg tgctgtgaag atggaaacaa acaaccaaat gcataaagca
     4021 gacattgagg accaatttgg caaacgcgtt gccaagctct tggattcaca aagtcaacag
     4081 ctagatagcg ctgttcaaga acggctattg caatcacgaa ctctagcaat aggtcgtgcc
     4141 aaaccagagt ccattctcgt cctagatcga caaatggcta ctgtaggcca tcgcctcaat
     4201 gcgccaaggc ctaattatcc tctttggtca tttggcacat ggctaatccc cctcgtagtt
     4261 gttgtgttgg ggctcattgc gattaccgaa tggcaagaag atatgcggat taaagatatc
     4321 gctacagtag atatcgccct tctaaccgac gatgttccgc caaacgcatt tgcagataac
     4381 ggctacatgg cctatctaaa aatgaagacg gttgccaagc ctgaggtgga agagaaaaaa
     4441 gcggaagacg agaaaattta gtcatgaaaa gagctgcgct ctcggcatat gttctgaccg
     4501 tgtcttgtct gattcttccc acggctaccg cagcccaaga gccaagcctc aatcaaaaaa
     4561 ctcaagagag tagctcgcga aaaaagggaa caaagttaga ttggccaaac ctaagtccgt
     4621 tacaacaaac tgttcttgcc tcactcgaat ctgattggaa taccttcaac caaaatagta
     4681 aagtgaaatg gttaaaagtg gcagatcgct acccctcgat gtcatctgca gatcaagagc
     4741 gccttcaagg gcgcatgtcg gagtggtcaa agcttcccca aaaagatcgg cggatggcac
     4801 gcgataatta cttatccagc ttgcaatttc cgccagagca aaaaaaggct gcttgggagg
     4861 cctatcaaca attaagcgaa gaagagaaac aaaagttagc ccaacaagaa cgtgctcaga
     4921 aaaaaacagg ggcggttagc tcaccagcga tccaacctcg gccggtactt aacaatacca
     4981 gtgccccctc atcaaacccc acaatcaaat aaactgaggg gatgaacccc agcgatctca
     5041 aagcactgcc ctcacctcga ttttggcgaa gggttttttg taatctctac gagcaattaa
     5101 tccttttggg ggttcttgcc ttcaccttcc tggtccccaa tctaatcatt ggtattgtct
     5161 ttggaatagc aatcccaagc tggctcacct tcttctatct gtatggggta cttgccctct
     5221 acttcacctg gtattggcgc cgtaatggtc aaactcttgc gatgcaaacc tggcgcatac
     5281 aactaatcac tgcagatggc tatcgccctg aaaaaaaaca nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnntcat gccgcttttc ctttgcgggg ctggcaaatc atcagcctac
     5401 tctttgtagt ggctcttttc ttatggccac tcagtattta tatcgatcgc aaacatcgtc
     5461 aaggcctacc cgatcgaatg gcaggtacaa aattgattga attacctaga tatccaaaaa
     5521 aagagcaagc gccaacaaag gcttagttat gctgcgcgca agcaatccat cgtgcttgcg
     5581 ctttgtattt tgcgttgtaa ctgagcaccc gcaatcaaac tcactagcaa gccaatcacc
     5641 atacctagag caattgcgct cgcaaatgag taaaactcaa tttctaggag atagcgaccc
     5701 aaggcccatg tactagcact agccgcaaga ccgccgagca agcctgataa caaaccaatc
     5761 gctgcgagct ccataaaaac caattgactt aatgtttgct tggaagtgcc cattgctttt
     5821 aacaaagcag catctcgaaa gcgatcatcc tgcgttgagc ccaaaaccgc aaataacacc
     5881 agaatggcag cgcaaagggt aaaggcaagc aatagtccga gagcgcttgc caagcgattt
     5941 aaaacttcct gaatttgcct caaagagtta tcaatatcga caattgtaag atttggatat
     6001 cgttgggcca actgaatatc caaggattct aagttcttcg cttggtgata agacgtaatc
     6061 caagattgcg ggaaactctt taaggcctcg ggcggaaaga tcacgaaaaa attaacctgc
     6121 attgatcccc aatcaagttt acgtaaagag gtgatgggag cagtaatcgt ttgcccagcg
     6181 atatcaaaac tcatttgatc gcccaagctt agtcctaagg tttttgcaat gccggtttcc
     6241 agagaaatct gtggcctact atcttcccca aaccaacgac ccgataccaa ttgattatcc
     6301 gtcggaaaaa tagatgtata ggaaagattg aactcacgat ccactaatcg cttggcatta
     6361 tcggattgat actgatcggg cataacctcg cgcccattta tttgcgtgag cctacctcgt
     6421 accatggggt ataactgcac ctggcctacc ccgccggtat ctagcatttg ccgaacagtt
     6481 tgccgttgat cgttttgaat attgatcaag aaacgattgg gcgcatcgac cggcacattt
     6541 gcttgccaac tctgcaacaa atcttgtcgc aggagaaaga tcagtagtaa agccatgatt
     6601 gccaaagcaa gtgagctcac ctgaatcatg gcaaaggcag cacggcgtga ctgcgcacta
     6661 atagcaaagc gaagagcaaa accaatatta gccaaatgac tagattgggc aaatcgacct
     6721 agactccaaa gacccaagta agctaaggct gcaaatgcta ttgcccctaa cccaaaacaa
     6781 actgtgaccc aaattaataa tttccaatca cgagttgccc agaaaattaa tgccaagcaa
     6841 ctaagcaggc caaatgcagc catcgactga gcggcaaatg gcaccttacc aaactctcgt
     6901 cgcactaagc ggagtggcga tatctgactt aaggtgagca tgggcggtcc ggcaaatcct
     6961 aataaaagta ataaggcaaa aataatgctc caaaccatag gccaaattga tggggctggt
     7021 aaattacccc ggattaaccc ccccaataac tgcatcaaac tttcttgagc aaaccaacca
     7081 atcgctgcgc cagaaaaact tgccactaaa ccaatggctg ccaataggta taactgccta
     7141 cgcaaaatca tgccttgact ggcaccaaag catttccaga ccgcgctaat atcggcttgc
     7201 tttagaacgt aacgtcttgc agctaaagca atagcaagag cacagattaa tgcggtcatc
     7261 aaagcaacaa tcgataaaaa ccgctcagcg cgttctaggg tcttgcgcat cactggctgt
     7321 gcattctcta aagactcaat ccggataccg cgaagttttt gttgctcaat gaagcctgtt
     7381 gcccatgttt gataagactt tatttgggca tcgcttccag aaagtaatag gcgataggta
     7441 acccgacttc caaacccgat tagcccggta cttgctaaat catctaaagc gatcatcacg
     7501 cgaggcgcaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgacctc
     7621 aatgacagag agatctttat ctttgcttaa tgtgtcctga cgactaattt gcaacgtacc
     7681 gcgcaatggg tatgcattac taaccgcctt gagtgagctt aatttacttt cacccccatg
     7741 gctaaccata ctgggaaata caaccgtttg cgcaatccct aatttctttg cgtttgcttc
     7801 ctcaatgagt tgagcggaaa taggctgatc tgcagcaatc aataaatcag atgctagtaa
     7861 agtacgcccg tctatctcaa aactacgttg taaacgatcg gccagaaagc tgacgctaga
     7921 taatgcggca accgataaaa cgagggctat aaaaagccaa aggagctctt ttgctcgaag
     7981 ctcctttgcg aaagttaaca tggcaattaa atactcggta cttgtccgcc atcgatgcga
     8041 atgaccgatc cagtgatgta agaagcattg gtacttgcca agaacgcaac ggtatctccg
     8101 tattctttag gatcaccata acgccccatc gggattgttt taaggcttgc actcactact
     8161 tgttcatagc taatgttctc gcgcttagca cgcgcctcat ccaattggcg taaacgatct
     8221 gttgcaacac gacctggcat gacaatattg acagtaacac cttctgcagc aacctcggct
     8281 gccaaggtct ttgaccatgc caataatgca gcgcgcagag tgttagagat tgctaaattt
     8341 ttgattggca caattgcacc cgaggtcgtg ctggtaatta ctctacccca cttacgttca
     8401 cgcatacctg gtaatactcg gtctgtaatg cccattaggg ataagaccat ttcattaaaa
     8461 cttttttgcc ataactgtgg gtcttgccca gtagcggtcg tgggtggcgg accaccagta
     8521 ttgttaatca atatatcaat cggacctagt gttgactcta cctctttcac atgagcatca
     8581 atcgaatcta ggttggccag atcccaattc agggctaagg cttttccacc cacagcctca
     8641 atcatctgaa cagtctgctg caaactatct gcattgcgac cggttacagc aaccttcact
     8701 ccctcgcgtg ccaaggcaac agccatggct tgaccaagac ctcggcttga ggcgataacc
     8761 agtgctgttt ttcctttaag tcctaaatcc atttcatctc ccttagtaat ttcaattgtt
     8821 tattctgaaa atatcttttt atttgaaaac agccttaata aaatgattat tctattaagg
     8881 gtaagcctaa tagttagtat taaaacaaac tatttaggct ttgaggaatt tgcccgtgga
     8941 gggcaaataa taggaatatg gcgcggctgg caggattcga acccacgacc ccttggttcg
     9001 tagccaagta ctctatccaa ctgagctaca gccgcatgag gcagaattat gccatggata
     9061 gcaagaacta cattaccccc attggtcatc aagccctcaa aacggagtta ttgcaccttt
     9121 tggatcagga gcgtccagaa atcgttcagg tggtccattg ggctgcatcc aacggcgatc
     9181 gctcagaaaa tggggactat atctatggca agaagcgctt acgagagatc gatcggcgta
     9241 ttcgcttctt aaaccaacgc ctagaaaacg ccgttgtggt taataactct gaccgaatcg
     9301 ccaacggtgg tgatcctgaa cagatatttt ttggcgctac cgttcaatac tgcgactctg
     9361 aagggattga gaccaccatt cggatcgtgg gggtcgatga ggttgatctt gaacgaggct
     9421 atgttagttg ggtctcgccg attgcaaagg cgctcatcaa agcaaaggtg ggggatacgg
     9481 tcaaaataca aactccttca gggttaaaag agattgatat tgttggggtg aactacgatg
     9541 agtcctagtc actttgctta ggcgctgcga ttacggatca ctctaaatac gtcagagtta
     9601 gtgcgcagcc ctcttaatac tcgggataag tgcaggcgat tagatacctg aatcgtaaag
     9661 cgcatcgtta cggagtcttc tttgtactta tcgtccatcg agacgttcaa gatgttcgaa
     9721 tcggctgacg taatactgct agccactctc gccaatacgc ccttaccttg ctttgtatct
     9781 actgtaatag cgacatcaaa ttctcgattg gtatctttac tccactgcac tcccgcccat
     9841 ttgtcactat ctttagagag catcctaaat gcaactttgc aatcctgtgt atgaatttgt
     9901 aggccctcgc cctttcctag gtagccaata atgtcatcgc ccggaatggg gtggcagcaa
     9961 ctttggaagc ttaccgacat tccctcgcga ccatccacca caattgcctg ccccagtggt
    10021 tcattatgta aggtactcca atcacccgta ccgagtcgca tttgttctga gccggcttcc
    10081 tctccaatga ggatctttaa gcgcgtagca agctcttgcg ccgttcttcg accaagcaca
    10141 atgttcacac acacctcttc gcgagtttta tcacccgtcc aatgcaacaa tttctcccat
    10201 aactcgggaa ccaataagcc agcatcaacc ccctgctgtc gtaaagcact ggctaataat
    10261 cgctcaccga gttgaagagc ttctgcatag tgttttgtct ttaatgaatg acgaattgct
    10321 gcacgcgctt taccagtgcg cacaaactct aaccagcctg gattgggttg tgatgaggtc
    10381 gagttaatca cctccacaat atcgccattc tttaactcgg tgcgtaaggg taattgcaga
    10441 ttattaatct tgaccgctac gcaggcattt cctaaatcac tatgaatgga gtatgcaaaa
    10501 tctaatgctg tcgctcccct tggtaatgca cggatctgcc ccttgggcgt aaatacgtaa
    10561 acggcatctg ggaacagatc aatcttcaca tgctccaaaa actcctgtga gtcgccacta
    10621 ttatcttgga tatcgaccaa agactgcagc cattggtgcg cacgattttg tacctcgctt
    10681 aactctggag aaccatcttt gtagacccag tgtgctgcaa ccccagattc agcaaccgca
    10741 tgcatatcag cagttcgtat ctggaactct accggcacac cggatggacc taccaaagtg
    10801 gtatgcaacg actgatagcc attcagcttg ggaatcgcaa tgtaatcctt aaacttccct
    10861 ggcatcggct tatagagcga atgtaatatg cctaaagcgc gataggtctc atcgaccgtg
    10921 tggacggtaa cgcggaaggc ataaacatct aaaacctggg aaaaactcaa gtgcttgctg
    10981 cgcatcttgt tatagatgct aaaaagcgtt ttttctctcc cctgtaaatc aacctcaatc
    11041 gctgccttac caaattgcga gcgcacactc tgcaaaatct tgtcgaccat ctcacgacga
    11101 ttacctcgcg ctttcttgac tgcgttttca attaccctaa agcgcatcgg catcgagtgc
    11161 ttaaagctta catcttgtaa atcacgatag atcaggttca aacccaaacg atgcgcaatt
    11221 ggcgcgtaga tctccatggt ctctagggcc acgcgacgtc tcttctcgac aggcactgca
    11281 tccaaggtat gcatgttatg ggtacgatca gcaagcttca ctaagatcac gcgtacatcg
    11341 cgcgccatcg ccatgaacat cttccgaaaa ctctcggctt gtgcttcggc atgactttgg
    11401 aactctaatt tatctagctt ggttaagcct tcgaccaatt cggcaacttt agctccaaac
    11461 tggttaacca gatcggtttg tgaataccct gaatcttcaa tcacatcatg caataaagca
    11521 gccatgatgc tctgagcgtc aagacgccaa ctagcacata actcagcaac ggagaccgga
    11581 tgggtaatat aaggctcgcc actgtgacga tactgtccta gatgagcagt atcggcaaac
    11641 tgaaatgccc gtttgatttg attaatttcc tctggcttga ggtaatgaag tttctcggtg
    11701 aggccggtga tcgagacaac ttgttgctta ggtggaacgg taggctgtga tgttggccca
    11761 aacaggtgcc gactcgattg ctcaagcaat gaagcaatca cactttgatt tgtactgaga
    11821 cccgccttag ctgagtttgc actcaattgg ctagacgaac tttcgcctag caaacccgtt
    11881 gtggtgactg ttgacgccac agcggggcct ccaatttaaa gaggaacttt ggtcaacata
    11941 tcccgatcgg ttacgccagc agcaatctca cgcaaggcga caacggttgc cttgtctttt
    12001 gcatccacgc gggctgcgtg accctgaacg agctgacgcg cacgataggt tgcggccaag
    12061 accaactcaa aacgattggg tacggttttt aaacaatctt ctacagtaat acgggccata
    12121 cggaactcac ttatttagat taggaggatc tccaacccct ataggataac tgattggctg
    12181 ggatcacgcc ccaagacgct taacgagtgc ggggtaacga gccatcatgg ggcttgcacg
    12241 caagcggctt gcagccacaa tggcttgcag atcccgcaaa gcgtcggcaa agatctcatt
    12301 aaccacgata aagtcggctt catgggcatg ctggagctca atatgagccg ctgcaacccg
    12361 cttggcaatg gtctctttat catcctgacc gcgtttataa aggcgttctt ccaaagcctg
    12421 tatcgacggt ggaaagataa aaatccaaat agcttgcggt attaattgac gaatctgttg
    12481 tgcgccttgc caatcaattt ctagaatgac atcgcttccc ttttgcattt gtccctcaat
    12541 ccatgagcga gaagtgccat aaagattgcc gtgcacttcc gcccactcta gaaaatcacc
    12601 tgaggatttc ttggctaaga attgctcttt agaaatgaat gagtaatcgc gcccatctag
    12661 ctccccagcc cgtggtgctc gtgtagtaca agataaagag agttgcaggc tgtgatctgc
    12721 ctcaagcaaa gcgttgacta atgacgattt gccggcaccc gaaggagcca caatcatcaa
    12781 catgcttcct tcatacgatt gcggggtcgt gttcatatta ataataggat ttactctaga
    12841 ttctgcacct gctcgcgcat ttgctcaatc aataatttga gttcgagcgc ggcgttggta
    12901 aagtccggat tgactgattt agatcctagg gtattggctt cccgattaag ctcttggatc
    12961 aagaaatcta aacgcttacc aacaggccca ccctcctgca gggttgtatg agcagtctgt
    13021 aaatgagtct ttaggcgcgc caactcctca gccacatcaa tccggactgc atagagaaca
    13081 acctcttgcc gaatcctttc catcaactcg gtattgattg gtatttgcac tgtcttggaa
    13141 tgagtaccaa gcgcttcttc taaacgagcg accaatttac tttgatattg agaaacgatc
    13201 tctggcattc ggggctctat ccgagtcacg atagcctcca tcgaggtcaa aatagtgcct
    13261 agcactaacg ctagagcctt accctcttct tgacgcgcac tcagtaattg ttttagagct
    13321 tcctgaccgg cttcaatact tgcgacctgc cattgatctt ctgcaatttc tgattctgta
    13381 acgacacctg gccagcgcaa aatgtctgcc attcgcagtt ccccggcatc cggaaactgt
    13441 tttttaatgc cctgctgcag ttcttgaaga gctgccaact tagcctggtt aacgctaata
    13501 gcggtttttg catggcctga ctccgattgc gttaaacgcc aaactgcccg aacctcgatc
    13561 ttgccacgct tgagagtttt atttaaaagc tctcggatac ctgattcagc actccggcac
    13621 tcatcgggca tccggaaact tatatccaga aaacggctgt tcaccgccct aatctctacc
    13681 tgtaaattaa caaagccccc cgaacccaac tgaatttggc gggaggcgct tccataacct
    13741 gtcatgctcg atatcatgta ggcattgtaa attgcaaagg taatctccca tgaatcagcc
    13801 cttccagcgt cctagccaac gtcaagcccg cgacctacga cccgtgaccg tcacacgctc
    13861 cttcacaaaa catgctgaag gctctgtcct cattgagttt ggggatacga aggtcttatg
    13921 caccgccagc gtcctagaaa aggtaccacc ccatcagaag ggatcaggag agggttgggt
    13981 gaccgccgaa tacggcatgc tgccccgctc cacccacact cgctctgatc gcgaggcggc
    14041 tagaggcaag caaagtggtc gcacacaaga gatccaacgt ctaatcggtc gagcaatgcg
    14101 cagtgttttt gatctcaagg ctctagggga acgaacaatt catttggatt gtgacgtgtt
    14161 acaagcggac ggcggcacaa gaaccgctgc gatcactggg gcttatgttg cagcccgcga
    14221 tgcagttaat ctcctactca aaaataaagc aattaccagc gatccaattc ttgatagtgt
    14281 tgcagcaatc tcggtcggca tctatcaagg cgtacccgtt ttggatttag attacgccga
    14341 ggactctgcc tgcgataccg atatgaacgt tgtcatgacc ggtaagggcg gcataattga
    14401 agttcaaggc acggctgagg gcgcgccatt ctcacgcacc gaactagagg ctcttcttga
    14461 cttagcccaa gccggcattg aagaactcag tgctctacag agaaaagcgc tcacctaaga
    14521 tgaatcaagg cctagtatta gcatccaata atgctgggaa gttgcgtgag tttgctgaac
    14581 ttctaaaacc atttgcgatc gatgtgatag cccaagggca actgaatatc ccggctgcag
    14641 aggaaccctt tgaaaccttt gtggataatg cgctagccaa ggcaagacat gctagcttac
    14701 tcagtgctct gccagcttta gcggacgact cggggatctg tgtcgatgcc ttaggtggtg
    14761 cgcctggagt acgctcggct cgttttgcag gggagcattg ttctgatcgc gataacaacc
    14821 ggaaactgat atcggcatta gaaaatcaac caaatcgtcg cgcccattac gtttgtgtct
    14881 tggcattggt tcgcagcgcc acagattcac aacctctcat tgtagaaaca cgctgggatg
    14941 gggaaatcat cgatacgcca cggggtgcca atggctttgg ctatgacccc tacttttacc
    15001 tgcccgagct gggcaaaact gctgctgagt tgctagcgga gcataaaaac caaattagtc
    15061 atcgaggtca agccttaagg aacctcatga gccaattaca aaactcttct ttctttacca
    15121 ccaatgcttg actctgcaat acagctcaaa gcattacccc cgctctcgct ctatatccac
    15181 tttccttggt gtgagcgcaa atgtccctat tgcgacttta actcgcatca ggtgaaagat
    15241 ggaggctttg atgagaagcg ctacattcag gcgctgattg cagatttaca aacagagctt
    15301 ccgaacattt gggggcggcg ggtacacacc atttttattg gcggtggcac acccagccta
    15361 ctctcccccg ctggtcttga tgaactgctc tcccatgttc gagccctaat accgctcgaa
    15421 cctcatgccg agatcactct ggaggctaat ccaggatcag tagaagctgc aaagtttaga
    15481 gaatttgccc agctgggggt caatcgagta tcgctaggga tacagagttt taatgatcgc
    15541 cacctcaaat ccttaggccg aatccataac gcctctgaag cgcaggctgc cattgaaatt
    15601 gctcaggact actttgcaag tgtcaatctt gatgtgatgt atgccttgcc agaacaaact
    15661 gccgatgaag caaaagccga tctactgcgc gccttgtctt tccaaacgaa gcatctatcg
    15721 ctctatcacc tcaccatgga gcccaatacc tactttgcaa gccatccacc acccctaccg
    15781 aacgaggatg aaagtgatct catgtttgag tttgcactct ccacccttca agagaggggg
    15841 tatcagcgct atgaggtatc cgcctactgt caagataacc aggtctgcag acacaatctg
    15901 aattactggg aatttggaga ctatattggc atcggtgcag gtgcgcatgg caagatctcg
    15961 atgccaaatc gaattactcg gcaaatccgt gaacgtcatc ccgaaacgta tatggacaaa
    16021 atcttcaaag agaactctgc ctgtcttgag aaccgtagca tcgagatcga cgatctggcg
    16081 tttgagttca tgttgaatgg tcttagatta atcaatggtg taccaaccca ccaatttaca
    16141 gaacgcactg gtctgccttt gcatcgaatc tctaaagcaa tcgatgaggc gattcgcaag
    16201 ggcttattag acgaggatcc gatgcgactg aaagcaacag cccttggtat gcgctatctt
    16261 aatgacctac aagaactatt cttgaaataa atagcattgg cggaagcggt gagattc
//
LOCUS       DDPQ01000091            8911 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5067,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000091 DDPQ01000000
VERSION     DDPQ01000091.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5067"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    283..333
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1288..1337
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2471..2616
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4261..4421
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4680..4777
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5125..5147
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6156..6187
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7385..7446
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7575..7701
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2235 a         1853 c         1736 g         2335 t
ORIGIN      
        1 ttgacaaaga cgagagtgat ttatcagtcc accaaaggag ttactagaaa gactttgccg
       61 aaacgtaatt tcttgattag tcttattggt catctgcgac ctgaaaaaga tcctttttgt
      121 cttgcgcgtg cccttccctt tttaccggcc tcaagcacag ttaagattaa gcacttgggt
      181 aaggcgatga gtcctgaaat gcgaacactg gcaaaacagt acagcgctca tctagcccgc
      241 tatcaatggc ttgacgagca aacccatgcg aagacgatgc agnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgcatg ttgttacgga ggcaattgcc
      361 attggggtcc cagtgattgc atcgaatatc tctggtaatc gcggcttact gggagatgac
      421 tatccaggct attaccccgt tggcgatgaa aagactctag cagctctcat actgcaagca
      481 gaaacaaatc ctgcctttta caagtctctt gagaaatccg ttcgccaacg acgtaaatta
      541 gtccaacccg agtttgagaa gcgctccatt aagaaacttc ttggtcagtg cttatagaac
      601 cacaatcgtg ttgcaacagt ttattgagag actttccctg gacgattagc aacgatttgt
      661 aacttagggt cgttatttaa aatgccaatc agctgccctg cgcttatctg tcccttaaaa
      721 gtatatgagc tagcaccatc cttaaattca aactcgattt gattaccggt aatccgtcca
      781 tcttcaaaat aaatcgctct tcgattactc gtgttaatgg aaccgtcaaa gaattgcttc
      841 ttttgtcgga ttactaaatt ggctttatct cctttcggta agccactcat aacccaaggg
      901 ccatcgataa tagcggggac tatccataaa taagccattt cgcccggacg cgttttaatc
      961 tcacgatcgg gaatccatga ctcgatccga aaggtgttag aaactaccct cgtcccaggc
     1021 ttcatcttta agatcgtggg cattaatcgg gtattgagac tctctcctaa aaacaaggtc
     1081 agaaccgttg cctgcgagaa gtcttcaaca aagatatcac cctgtttaaa actgactaag
     1141 ttttgtacct tagcgcgctg tgcattacga atcgaaagcg ctacaagatc tgggttgtac
     1201 tcaatgccat aagcacgaac accatacagc ttagcagcct cgattggaat gacgccatca
     1261 cccgagccaa gatcataaag aatatcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnntgc catgcgaagc tgaaactggt catcgccgta gttagtagaa
     1381 gaacaggcac cgatacttgc gagaacaaag ccagtaagac caatggagaa taagcgattt
     1441 atgagcatgg cattaataaa tggtgatctt acttattgaa agatgtctat gataatgaga
     1501 tcaaatgtcg acgcaaggga tattaaagat tcatctaaac aaaggggttt ggtgcgcatt
     1561 agacagactt aagtggacta tcataggcca taacttggga ttattgaatg actaagcaga
     1621 gcaaccagac taatccccgt gggtttcgga ccttagcggc actcctccct tttctgaggc
     1681 cctataaaaa gcaatttgtt ttagcaatct ttagcttaat actcgctgct agcgcaacct
     1741 tagccgtccc cttgtccttt aggcagatca tcgatttagg ttttaacgct aatagtgaca
     1801 accaaatcaa tcagactttt ttaatcttat ttattgttgc ctgcttagtg gcattaggaa
     1861 catctcttcg tttttatatg gtctcatggt taggcgaaaa gattacgaca gatattcgtg
     1921 cggctgttta tagccatgtc attaaacaaa gtccagagtt ttttgaatcc actcatagcg
     1981 gtgaaatact atcgcgtctt aataccgata cggttctaat ccagacatta attggcacta
     2041 gcgtctcgat ggcgatcaga aatattcttt tgcttgcagg gggtttggtc atgatgttta
     2101 ttaccagccc aaaattatca gtgctgattt ttgctacgct actactaacc atcatcccaa
     2161 ccatgattat gggcaggcgc gtgagaaagc tctcccgggc ttcacaagat aagatcgctg
     2221 atgcctctgc tttagcggga gagaaattaa atgcgatacc tacgattcaa tcatttacca
     2281 atgaaaaagt agaaattaat cggtttaatc aatcgattca aactgcccta gatgcagcta
     2341 ttactcggac gcgagcaaga tccttgctca catttgttgc tattttattt ggctttacca
     2401 gcattatttt ggttctctgg ctaggcgctt atgcagtcat ggacaatcaa attaccgctg
     2461 gagagttatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgc aaatacaaaa tctcagcttt
     2641 cgctacccat ccaatccaaa tagtgtttta tcgaatatat ctctcgagat taaaccaggc
     2701 gaaagagtcg caattgttgg tccatcgggc gctggcaaga ctaccctctt ccaattattg
     2761 caacgctttt atgacccaac gagtggaaca attcttttta gtgacatcaa tattcaaaac
     2821 attccgctcg aagcactcag aaaaatgatt ggtgttgtgc ctcaggatat tgtgattttt
     2881 tcagataacg ccatggaaaa tattcgtttt ggaaaaatgg atgcaacgga tgaggaagta
     2941 ctatctgcag cacggctcgc aatcgctgat gagtttattt ctaagcttcc ggatggctac
     3001 caaagcttct taggggatcg tggtattcgc ttatcgggag gacaaaaaca gcggattgcc
     3061 attgcaagag tcttattaaa aaatcccgcc ttattactct tagatgaggc taccagtgct
     3121 ttggatgccg agtctgaact actcgttcag cgagcccttg aggcagcaat ggatagccgg
     3181 acaactttgg tgattgcaca ccgcctgtct acagtaaaac aagccgataa aattctggtg
     3241 ctagaaaacg ggaagattat agaaaccggc actcatgctg atttaattca acgaagtggt
     3301 ctctattccc gtttggccaa gcttcaattt acagaccaat aaataagcat ctcatcatgc
     3361 agtatcctag aatctcatat tcatgatgta ttaattcatg tgtgtttatt tagagcgaaa
     3421 cctaccatgt caccttggaa agcagccctc cccggcgatc accttgatca aatcgatacg
     3481 cctgccttaa ttcttaactt ggatgccttt gagcgcaata tgcagcgctt gcaagacgca
     3541 ttatctggca caggcgtgcg cttacgtcct catgccaaaa gccataagtg ccctgatatt
     3601 gcattacgtc aaatccaagt gggtgcagtt ggtatctgtt gtcagaaagt gagtgaggca
     3661 gcagtctttg tagaggcagg tgtgcaggat attttgatca cgaatcaggt ggtcggtgac
     3721 aaaaaagtag cccatgcgct tgatttggct aagcgtgccc gaattggcat tttggttgat
     3781 cacgaagaac aggtatccgc cttcgcactt gccagcaatg aacgtcaaat aaaaattgat
     3841 gtgtatatag aaatagatgt tgggatgggg cgttgcggtg ttgcatcgac tgaacggaca
     3901 gtatccattg ctcggcaaat tgatcaagcg ccctacttaa actttatggg actacagtgn
     3961 ngctgccacc aaagaagcca ttgaaaaatt ggggattaag gttgaacgta ttacgggagc
     4021 tggtacgggc tcggtcatgc tggagcgcga ttccaagcta tttaatgaag tacaggcggg
     4081 ttcctatatt tttatggatc gcgactacgc tgccaaccag cgagatactc atgatttgcc
     4141 gtttgaacat gcattgtttg ttaaaacagc gatactgagt catcccatac aaggccgcgc
     4201 agtagtcgat gccggcttga aagcgtcgag tgtggattca ggtatgccag ctatctggca
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntccttgtct tattcagtgg
     4441 aataacctga accggaatca ccaaggagtc aaacctagcc tcatctatga gggccacttt
     4501 tgggcacatt gatatgccca tatgggcaat ggcggcaatc attgccacaa cagatgcctc
     4561 gctttaagtg tgcctgactt gtaagtactt gatatcctga cgcagggtca atataggtat
     4621 ctagtccttg ttcacacgca agtcgatgga gttcttcaat gctttgcatg gtcttatttn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta gttttgggtt gatagcctag
     4801 gcaggctgat ccggtatttg ccaaaacttg agagtctggg cttagcttag atttaatttc
     4861 atatacctta catccatagg gaaacttctt atcccaggta atgtagaaaa atttacattc
     4921 tttacagttc attccaaaac cataacatca atctgtaagg gcctgtcatc ttgcgctacc
     4981 aaagaaaatg ccccgcataa agcagggcat tacttgaatt acgctgtctg gcccacaatc
     5041 aaccgaggcg caaatcagtc gtgcttacgc aaagccatcg ccaccaaaat cgctatcaaa
     5101 atctgctaaa tcttgatttt ggtcnnnnnn nnnnnnnnnn nnnnnnntga cctaacatac
     5161 tctgaatgcc ttgataaagc atggaaccag cgaccacacc tactgccgta gtcgcaatgg
     5221 tacctagcat cccagatccc catgcactgg gttgcgcagc gggtgcgcca ctcggtcttg
     5281 ctggtgaaat tgcattattg ctagtcgtag ggcttgccgc ccttccccag gaattattat
     5341 ccccaagaaa acttgcattg gatttaccag ctacttgggc ttgaagctcg gctatttgtt
     5401 tttgggcaac ctgcaaggcg agctcaagac ccatagcacg ctgcaccagc aggtaggcag
     5461 catcagcttg atgttttgtg ctctccacta ttaactcgtt tgcctcacta tccttctgaa
     5521 cagtttgtgt tgcttttaat tgcttcagaa aattctctaa catctctttt tcattgggat
     5581 tcataaaggc tccgatcgga aagtaatggt ttggatgtat cttctactgt caggtgatac
     5641 ttcaaatgat atcttttact tatttaaaat aaatactggc gctaataccc actgcaataa
     5701 aactgttaat ggtctttatg taccaagagc cccttagcca accagcacga tttaacagtt
     5761 cagccattcc cttggaaata attatgacag ttcgattagt catttaattc aatgatttag
     5821 ggtcaaaaga cttgatgatg acggataggt ctagattgcc cgcaaaagga tgtgttctag
     5881 cccagcctct acaatccaat catgttcata caaatcatta atctgatact ttccgttgtt
     5941 gtgggaattg tggcgggggc ctgtctcttg cgttgctata tgcaatggtt tggctctcag
     6001 cttggggcag agcaacgtaa gttaattgga gtgtatttgt ttccactcac aaactggatc
     6061 gtcattcccc tgcggcgcgt aatacccagt attggcctct tggatacagc tagtctctta
     6121 gcggcttatt tactggtttt tggcaaggtc gcgatnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnntat ttgtctatgt gattctgtcg tggcttagcg ctggctcaca aacccaatac
     6241 attgtttctc ttttgatagg gccactgctt gggcccattc aacgaatgat tccgaagctg
     6301 ggagccctgg atctatcgcc gctcatcctc ttactcgttt tgcaaatcct acaaattgtg
     6361 atcaatagtt ttaagtagga cgcccagacc aaagccgccg tctgataccg caaccaaccc
     6421 tactcaacag aatatggcca tgatcactaa ctaaattgaa ctcaagaaga aactttattt
     6481 gtacctgcag cgtttactta ttatgtttgg caggaccatc tcgcgcaaaa ggagtacata
     6541 tggatttcag taaccctaat accttgcgcg atagctggat tgtgaatgat ggtgtgatgg
     6601 gtggcgtgtc tcaatctagt cttcgccaag atgaagatgg tatgtttttt gaaggcatgg
     6661 tttcgcttga aaataatggt ggctttgcct ccatgcgctc atcggtacga tttccccaag
     6721 gaactcaact gattgagctt atagcgaaag gtgatggcaa gcgttataag ctggtcttgc
     6781 gtaccgagct agcaccacgg gttacatatg tagcagattt catcgccctg cctacttggc
     6841 aaacgtatcg atttaattta agtcaattta aatcgacctt ccgtggacga gatgttaatg
     6901 ccccaaccct atccttttct gatgtgattg attttggcat tctgatttct aataaccaag
     6961 caggaagctt tgcgatccaa ctaaaaacac ttaaatcctc ttaggccgag gaaagcaaaa
     7021 ccataggctc taaggcttac tttgatttgt attgagtcgg gcaatatgcg cgctgaacag
     7081 aaggatcacg tagttcgcga tgcttggttt gacgtccata aactcgtttt cgccatagtt
     7141 catatgaact acgttttaga ctcttacgca tgagcgcttt aacttgagct tcagacaact
     7201 taaactgagc ctcaattgcc tcaaatggag tgcgatcctc ccaggccatt tccacaattc
     7261 gggataaatc agattcttga atagtaatca tcaggctaat taaacacgaa attgacaaat
     7321 caagtacagt cgcctatcaa tgcccttatt caataaaccc tttcgcgctc tgattattgg
     7381 ctccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnttgc atcgcaactc agatcctgtg attgattatg atcacctaga gacaatacaa
     7501 actgcctatg aaacacttga gaagcttaga ccctttgatc taattatcaa tactattggg
     7561 gttttgcata cccannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nggtggcgtt tttgctaccc tttctgccaa agtgggcagc
     7741 attactgaca accggctagg cggctggtac agctatcgtg cctccaaagc agccctaaat
     7801 atgttgatca aaactgcctc aattgagcta catcgaactc agccacattt agtgttggtg
     7861 gcaatacacc ccggtaccgt gaaatccaat ctttcgaggc cctttcgtgg ggatgagatt
     7921 ggtcgagatc ctagcgaggc tgctagtgaa attctttcta tattgggaaa tcttcaagca
     7981 tccgatactg gctcgttcaa gtcttatcgt ggagaggaca ttccttggta ggcacttagg
     8041 tcttactact taagaattgc gccatccgat agccgcttaa ccaagcacct tctacagttg
     8101 agttttttag ccaatcccca caaagtccaa ccttgatatt ctcatcatag acataagtca
     8161 attcactagg cgtttccagt tttgcgtatc gccataaatg actttgatat aaatcgcaat
     8221 cctgatctgt gagatcctta aatgccttca ataaaatagg ctctatttca tattgattga
     8281 agtcaatata ctcttgcgac cacgtattac tagcttgggc tacccaagtc tcatagcttg
     8341 accgaccagg ctttgtatta tctctagcaa tccaagaaaa ggggctgcct tcaacaaagg
     8401 cgccatcaaa gtctaggtct agaggtttct taaagtaagc aataagagtc caacaaggca
     8461 acatcaccac atctttacag cttgtgtcca agctttctga atatggacca attaatgctc
     8521 tagcctgaac cgatggtatg gcacatatca gatattcaaa tgcaagatca aatggaccct
     8581 gctcctttgt attaatgcgc cataaattat taatacgctt gagaccaata acagtatgtt
     8641 gagtatgaac atttagtgag cttgctaaat attttgctgg agcagtcata gcgggcactc
     8701 caacgaagcg ttgcgttgtc gactcaagtg tacgtatggt gctgcgatcg agtgtcacaa
     8761 tcttaccgcc ccaaggcgca atcacctttg cgttcatcca ctctgtaact tgggtaccaa
     8821 ataatgggtc tttaaccgtg aagtactggg caccatgatc tgcctcccag gcttcatagt
     8881 atcggtgact cattcgacca cccacgccct t
//
LOCUS       DDPQ01000092           36789 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2624,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000092 DDPQ01000000
VERSION     DDPQ01000092.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 36789)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 36789)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36789
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2624"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1415..1496
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6011..6022
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9467..9571
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11887..11954
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20013..20068
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21457..21572
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25896..25910
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9667 a         8068 c         8936 g         9662 t
ORIGIN      
        1 aggtaagatt gatcccaatt cagtcaaagt ggtttatatg aaatccccct ttgtggattt
       61 aactccgcgg ctaaagtcag caattactcc aacgggtatt aatttctcta aggcagaagt
      121 tccacctggc actcatacca tacgtataac agtaaaagat atggacggca gagaaacaaa
      181 tcaagttata acgatagtgg tcaataaata atgaaatgtc cttattgttt ttctgaggta
      241 gatgaagaag cagtagcctg taaattttgt actaaggatt tatacctctt caagccaatg
      301 atgaagaagg ttgctgattt agagcgccaa ctagaggaca ttcctgatcg cgatgcatat
      361 gagcgacgaa ttactgagct cgaagagatg cttagcgagc gcgaagatga ggtagcaaaa
      421 gaacaaggca cattgtcttc cctcataaaa aatattgggc tatacattgt tatcccctat
      481 ttttgctatt gatggcccat gctttaatta ttgtggtgta tgacacaaaa gagctgtatc
      541 tcagaatcat ttctatgatt ctgcccttac catttggata tgtcttgttt aaagccaaaa
      601 agcactctgt ttttccttgg tttattggag tggctttcct ggccatagcc tctgtaattg
      661 gaatgagctg gattacctct ttggtagata aaacgccaat acttccgcaa aatgcttttg
      721 agtggcgaga gcttcttcaa tattcagcaa gtattgcatt cagtttcttg actggcatgt
      781 tattaggcgg cacagcattt tttgcaaggc agagtaggcg tcgctcaacc ccaatcaatc
      841 ccctcatcaa agcaattgct tcgggtttaa gtgacgggaa aatatcagcc tctaatattg
      901 aaactctaat gaaacgtctc caaggatttt ttggaactgg agtcgcttta gcaacaacag
      961 caatttcgat ctataccggc ctaaaaggaa ttattggcgg ctaattagtg atgccttgac
     1021 tgtaactctg actgtcctaa aatagggatg atttctttaa atgcctgctt atttgattgg
     1081 attctgatat gagccacttt ttccataaat ctaaatttct agccttgata gccgccttgc
     1141 tcttgggagc gtgtgcgatg ggtgacagtc ctacgggttc tcaagatgaa atcaatcaga
     1201 ttcaatcgca gctgctaggc gatatgcctc tcccaacagg cgccaagatc atgggctccg
     1261 actctttaat tattggtcgt ggtgatggat gggttggtcg tgtcaatatc agtgccctgc
     1321 aaggcgctac cgatgtgtat gcattctttc agtcagagta ccccaaagcg ggatggacaa
     1381 cggtgactgc aactaaagca aaaacaagtt tcttnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccga
     1501 agaacgccaa cgtgattgcg ccgacgcgta agccctagtt aagaaaagga tttagatacc
     1561 gttggcacgg atcaagccaa cggcttgtcc ctcaagggta aagtcagcct cagccgtaac
     1621 aataatgttt ttgaactcag ggttttcagc ttgcagttca atgtagctcc cgcttgaatc
     1681 tttcttgcgc tgtaagcgct tgacggttac ctcatcatca attcgggcaa ctacgatatc
     1741 gccattccgc gcctcagtag ttttcttcac agcaagatag tcgccatcca aaattccagc
     1801 atcacgcata ctcattccct tgacgcgtaa taaatagtct gcacctttac taaacaggct
     1861 tgggtctacg ggcacctgtt tttcaatatg ttcgatagca gtaatggggc aacctgctgc
     1921 aacacgtcca atcaatggca gggttaattg ttgtaaaccg ccggacggta aattaagttg
     1981 tctaaagtta ttggccgacg agtgattgtt ggagtgcgtg atacggatgc cacgggatgt
     2041 accagggctt aattcgatat accctttttt ggcaagagct cgcaaatgct cttcagccgc
     2101 attggcggat gcaaaaccta agcggtttgc aatctcagcc cgagtggggg gtaagccact
     2161 ctcttcaata gctagggtga tgagacccaa aatctcctct tgccgagcgg ttagcttagg
     2221 tagggtggtt tctatcaagt catttactgt gtttatgttc atactgggat tgtatacagt
     2281 aaaaattagc atttcaagaa cttttgtaag gcttgataat gcaaatatga ctgatttatc
     2341 aaatacaccc ttgattttgc tcattggcat gggcggcacg atcgccggcc tccaagtgaa
     2401 cggggatgga aagggctacc tagccggcca agtgccgatc gagacccttt cggggcagat
     2461 taaaagtaaa ttatctataa aaaacaaaca gttatctaat attgatagct gtgatatgac
     2521 cgagtcttta ttaactcaac tgggtcagga ggtcgttcaa gcgcttcatg atcctcatat
     2581 tgtcggggtg gtcgttaccc atggcacaga taccatggag gaaacagcac tttttctaga
     2641 gcttgtttgt gggcatttag caaagactgc gggcaaaaaa gtggtgatca cgggcgccat
     2701 gttgccagca gaccacccta aggcggacgg cccagaaaat ctcaatcggg cagtcgctct
     2761 agtagaggat caggatgggc caagtgggat ttttgggttg atgggcggga aggtctttca
     2821 ggcgaggagt atggcaaagc gtcatacctc aagcttagat gccttcatga atagcgagcc
     2881 agttaatttg gatcttagca gtcaagttag cgaagactta cctataccat caatagataa
     2941 ttggcctaga gttgaaattg tgaccaatca cattggtgct aagggcgacc agattgaggt
     3001 ctataaaaag ctgggtgtgc tgggtattgt cctggctgga acagggggag gaaccgtcca
     3061 tcaggatcta gcgctcagct tggaagactt catgaaaggt ggcggggctc tagttcgatc
     3121 gtcgcgagta ggtttggggg tgattcctag gcaattagcc aatggtcgcc tagacgcatc
     3181 tatgggggct ggtgatttga acccgcccaa ggcacgtatt gccctccagt tggccctatt
     3241 cgctagtctc cacgcaaaag ctaatgcatt gagttggcag gatatttttg ctagaatagt
     3301 ggtcttgcct aattttaggt aaggtttttt acaacttgtt ccacccttgc tgcactggtg
     3361 ctgatttcta gacagcggaa attagcaaga cgcctaggca gttttatcca taaggagtga
     3421 ttaatgcgtc attatgaaat cgtttttatc gtccatccgg accaaagcga gcaagtacct
     3481 gcgatgatcg atcgctataa gtcgattctt actactcatg gtggtaaggt gcatcgcatc
     3541 gaggattggg gccgtcgtca aatggcttac atgatcgaca aacttgctaa agcccattac
     3601 gtttgcatga acattgagtg tgaccagaaa actctggaag agctcgagca tgcatttaag
     3661 tttaacgacg ccgtattgcg ccacttgatt gtgaagttga agaaggctga agttgagcct
     3721 tccatcatga tgaaggaagt tcagcgcgaa gaggctcgca aggttgccca ggcggatgcg
     3781 cctgctgcag cttattaata aagacggagg cttaaatctg taagcagtgg tctgattgtg
     3841 aagcgtagtt caaaccttct caccctgact gcttccttaa tcgctaaaga cgccattcga
     3901 tatacgccag ctggtttgcc agtgattcat tgtcagttac atcatgatgg tgaagcatac
     3961 gaagcaaatc aaatgcgcca aattcagatg aatgtcgaag caattgcaat aggtgagatt
     4021 tataaaagcc tagagcaaat ggatttaggg gcaaatgcag tgtttgaagg gttcttgact
     4081 cagaagacct tacgaaatga gcgccttgtc tttcatataa cgaagattga attgattgct
     4141 aaataggaaa ttattatggc ttttggaaaa acaaacaaaa agacagactt caaaaagaag
     4201 cctgcacaaa acccactgtt caagcgtaag cgctattgcc gctttacggt tggcggtgtt
     4261 gagcaaattg attacaaaga cgtcgagacc ctaaaggatt ttgttggtga aaatgccaag
     4321 atcactccag cgcgtttgac tggtaccaaa gctttgtatc agcgccagtt ggatacagcg
     4381 attaagcgtg ctcgcttctt ggcgctattg ccattctctg atcaacataa gaagtaatta
     4441 ggagcccact atgcaagtta ttctgcttga aaaattaacc aatctgggca accttggcga
     4501 tgttgtgcgc gttaaggatg gttttgctcg taactttttg atccctcagc gtaaagcgcg
     4561 tcgtgctacc gaagctgcga ttgcagattt tgcagctcgc cgtgctgagc ttgagaaatt
     4621 ggctgctgag aagcttgccg ccgctcaagt cattggtgat aagttaaagg gcctcgtcct
     4681 cgagatcact caaaaggctg gtgtagatgg tcgtttgttt ggctctgtga ccaatcatga
     4741 cattgctgaa agtttggcag ctaaaggctt ccaaattgag aaagcatctg tgcgtatgcc
     4801 tacaggtcca ctgaagttag tgggtgatca tcctgttgcg attgctgtgc atactgatgt
     4861 tgtggtggat attaatattc gtgttattgg tgatcaagcc tagttgactt cggtaaacta
     4921 gtcgtatggc tgaatcccgt aaccgtacta tttcaatgac cccgggaatg tctggggcag
     4981 gggacgcagt tgtacaggca cttaaggtgc cacctcattc cgtcgaggct gagcaatcag
     5041 ttctcggcgg tttgcttttg gataacaccg cttgggatcg actgggcggt attctctccg
     5101 aaaatgattt ctatcgaccc gagcactccc tgatttaccg agtggttcag cggctaatta
     5161 gtgataaccg tccagcagat gtcattaccg tttatgaatc cattaaaacg gagcctgagg
     5221 ctgatcttat cggcatcgat tatctcaact cattagcgca taacacgccg agtgcagcca
     5281 atatcaaagg ctatgcagag atcattcgtg atcgcagtat cttgcgacgc ttaattcagg
     5341 tatcggacgg gattgttaac tctgcattcc tgactgaggg ccgatcggtt caaaccttgc
     5401 ttgacgaagc agagtcacgc atcttgcaga ttggcgaaga gggtaatcgt aaagcggatt
     5461 acttagagat tgagcccctt cttaaacaag tagttgccag aatcgatgag ctctataacc
     5521 gtgatggcgg aagcgatatc actggtattg cgaccggatt tattgatctg gataaacaaa
     5581 caagcggttt gcaaaaaggt gatttggtta tcgtagcggg aagaccttca atgggaaaga
     5641 ctgctatggc actcaacatt gcggagaatg ttgccctcaa tgagggttta ccggttgtag
     5701 tgttctcgat ggagatgtca ggcgcgcagt tagcaactcg tcttttaggt tctgtagggc
     5761 gcgttgacca aagtcggatg cgtactggta aattaggtga tgatgagtgg ccacgggtaa
     5821 ccgatgctat tgcacgttta agtaataccc aaattctgat tgatgaaacc ggttcgctta
     5881 cctgcttaga gttgcgtgca cgggcgcgac gtattgctcg caactacggc ggtaccttgg
     5941 gtatggtcgt ggtggattat ttacagttaa tgagcggtag cacaggcggt agtggtgaaa
     6001 acagggctac nnnnnnnnnn nntgccctgt cgtggcactt tcccagttaa accgtggctt
     6061 agagcaaaga cccaataaac gacccgtgat gtcagactta cgtgaatcgg gtgctattga
     6121 gcaggatgct gacctcattt tgtttattta tcgtgatgag gtctaccatc ccgacaccac
     6181 aaccgataaa ggcattgctg aaataattat cggcaagcaa cgaaatggcc ctattggaac
     6241 agtccgctta agctggcaag gtcaatttac aaagtttgat aacctcgctg caaataccgg
     6301 cgcctcctat ggcggtggtt tcacaccctt ttaattagtt accggtcgtt gcgctagcca
     6361 cacgtcttgc ttcggtatag cgtgaagccc aatagggtgc atcaattcga tctaagcgaa
     6421 cagtgccacc tgcactagga gcatgaacaa agcgaccttt acccacatag attccagcgt
     6481 gcgagtagcg ctcacccgta gtgttaaaga agaccaaatc acccggtgct ggggcttgct
     6541 gaccaattcc acgcccagca ttactcattt gctggatagt gcgtggcagc tgaattccag
     6601 agcttttatt gaagacataa gcaattaaac cgctgcaatc aaagccaccc gcgggggtat
     6661 taccaccata gcgataaggc acgcccacaa gcccaattgc tgcgatggag atgtcttcac
     6721 tgccaacgct cgtatcattt ttgaagtgtg caatccttgg gctcgatgag gcctcactgg
     6781 aagtacgagg gctaaaggtg ctgcaagcac tgagcgttga cacactcagt acaagcaggg
     6841 caatccatcg agcaggattt ttacaaagct tcagcagcat ggtcagctag tcgcgaacgt
     6901 tcgccacgag ccaatgtaat gtgaccacta tggcgccaac ctttaaaacg atcaaccaca
     6961 taggtgaggc ccgacgaacc ctctgtgaga tagggtgtgt cgatctgagc aatattgccc
     7021 aaacacacaa tcttggtgcc cggcccagct cgcgttacca aagtcttcat ctgtttagga
     7081 gttaagttct gcgcctcatc aataatcaga aacttactca caaaggtgcg accgcgcatg
     7141 aagttcatgc ttttaacttt gatgcgtgaa cggattaact cctgagtagc agcacgaccc
     7201 cattctcctg cactgtcatc gttgcgatgc aagacctcaa ggttgtcatc aaaagcgccc
     7261 atccacggct gcattttttc ttcttcagta cctggcaaga atccaatatc ttccccaact
     7321 ggaacggtcg cacgggtaat gatgatttcg ttatagcgtt tgctgtctag aacctgctct
     7381 agtccggcag ccagagctaa tagggttttg ccagtaccag cctgacctaa gagcgtcaca
     7441 aaatcaatat cagggttcat gagcaagttc atggcaaagt tttgctcacg attgcgggca
     7501 gtaatgctcc acacattatt tttctggtgc gagaaatctt ttagggtttg taataaggca
     7561 gtcttaccgt taatctcccg aactaatgca taaaaaggag tagagccgtc aggattttct
     7621 tgatagacaa attgattcac caacatactg ggaacaagtg gcccagtcac tcgatagaac
     7681 atagtgccac ttttgccgtc tgcccaactt tccattgcct tgccatgttt gggccaaaaa
     7741 tcagcgggca gggtcatgac ccctgaatac atcaggtcac ggtcttccaa gacttgatca
     7801 ttgaaataat cttctgcagg caacccaaga gcacgcgctt tgatgcgcat attgatatct
     7861 ttggagacta agactacttc ttgattgggc ttcgcctttt gcaggtcgct taccacaccc
     7921 agaatgagat tgtcaccttt gccctctggt agtccttcag gaaggggtgt acttgagaaa
     7981 tgagtttgga agaacaggcg cccagtcgca tcctgatttc ctaatttatt cagcggtata
     8041 ccctcatcga gttgaccgct agtaccagcg ataagttgat ctagggagcg actgaccata
     8101 cgagcattgc gcgcaacttc actcatcccc tttttatggt tatccaattc ctctaaggtg
     8161 gtcatgggta aatacaaatc atgctcctcg aagcggaaca gagaggatgg gtcatgcatt
     8221 aatacattgg tatctagtac aaaaagactg ggggggccgg tacggatggt acgcttaggc
     8281 cgttcattct tggcttctaa tttcttttct aagtattgtg ctggtgcatc cgttttgatt
     8341 cgctcaagcg cctcttcggc agcggataga tcgtgttcca cttcttccac aacatctccg
     8401 ttttcaaccc agactgagct agtagctaat ttcttgacct ttttgggagg gtttttgaga
     8461 tcaggttgtt tctgatgact caaattcact tggtcagcaa tttgggtagg taggggaggt
     8521 aatggcatgc aagcttctcc taaatgaaaa aaccgcctag ctgatgctgt gggcggttta
     8581 ggggggtgcg aggacttcgg cggtatgcac agattttgag gggactagga cttgggcccc
     8641 gtctaaggag acctagaagc tgattaatcc aatcccatcg cttacggtgc atatatccat
     8701 actgtacccc aattttgtgt ctttgcaagt acagtaaaaa tacctagtcc agtttcttgg
     8761 cggcagctaa aacctcagcc acatgaccag ttaccttgat accccgccac tctttttgca
     8821 gaactccttt gctatcaaat aagaaggtgc tgcgatcaag cccccggacc tgtttgccat
     8881 acatattttt cattttcatg acaccaaaga tagcgcaaag cttttcttca gtatcggcaa
     8941 ccagctcaaa gggtagatca agtttttggc gaaagttctc atgcgagcgc aggtgatctc
     9001 tcgatacacc aacgaccaaa gcattcgctt gattaaagga atcaatgtga tctctaaatt
     9061 cgccagcctc gacagtacat ccaggcgttg agtctttggg ataaaagtac aagaccaatt
     9121 tcttgccttg ataggctttg ggagagaagg tgagttgtga tgttgctggg atctcacagt
     9181 acggtatggt ttgcccaatt ttgatagtca tattacattc cttcattagt attcattaaa
     9241 aatctgtgct tgtactagcc tgcaccatca actcgccact tcgattagca atacttccgt
     9301 acttaaattg ctcagtcgct attttatcga gctgtccagt agctgcccaa gaacgatgta
     9361 atacccccat tgtcacaagt tcatgtcctt gctccagcca tcctaggagt agtttgcgaa
     9421 aggccggtag cagcttttga ccttcgagct ctgcatgcag agtaaannnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgataagg aaccaaattt ggggcagagc
     9601 gaccatcgga agcatagagc attccccaag cgtcaatttg ctctaaagca gcaagattca
     9661 tttgccagcc tgcggcacca tgggttactg gagggtgacc aaagatctcg ataaaacgat
     9721 cgtaggcttt ttgcatctgc tgacgcgtcc acgaggcatc acgctgatgg acattatctt
     9781 gccaatacac atgatcccat gtatgaatcc cagtctcatg tcccgcttga tccaccgcac
     9841 gcatttcatt ggcagctcgt ttgccaatgt caggagcagg cagtaaaaca ccatacaaga
     9901 gggtctttat gccatagtgc tcaacaaccg atgtgcgcga aacttttttt aagaatccag
     9961 gtctaaatac acgctttagt gcccaacccg tatgatctgg gcctaagcta aataaaaagg
    10021 ttgcacccac cccgagttca gcaaagagtt tggctaagtt aggggtgcca acttgagtac
    10081 ctaacagggt atcaacatcg acttttaggg cgatcttggc catcaggaat aggatctagt
    10141 cgacgagatg acgggcttta tcaacatcat tgcgataggc ttcaaagatt ttggcaagag
    10201 cgtcttccat cgcagtactt ggcttccagc ccagctcttg catcgtattg ctaatagcgg
    10261 gcacacgatt ttgtacatct tgatacccaa taccgtaata ggctttggat gttgtctcaa
    10321 tcagctttac tttgtcagcc gtcacggcgt attccggtat ctttttcgca atcgttagca
    10381 tcatattggc caattctttg accgagtgat tattctttgg gttaccaatg ttatagatct
    10441 tgccattggc aatatcattt tcattggcaa tgatatgcat taaagcgtca atgccatcat
    10501 caatatacgt aaaggcccgc ttttgtgagc caccatccac gagattgatc ggttcaccgc
    10561 gcacaatatg acctaagaac tgagtaacta cgcgcgagga tccttctttg ggagtgtaga
    10621 tactatcgag acctggccca atccaattaa agggtctaaa tagtgtgaac cgtaaccctt
    10681 ccataccgta gccccaaatc acacgatcca ttaattgttt tgcacacgca tagatccaac
    10741 gtggtttatt aatggggcca tagaccaaat tagattgtgc tggatcaaac tcggcatctt
    10801 cgcacatgcc atagacctca gaggtggatg ggaagactaa atgtttgccg tacttgaccg
    10861 cggaacggac gatggggaga tttgcttcaa agtcgagctc aaaaactcgc aggggctgtt
    10921 gtacataggt agctggggtc gcaattgcca ctaggggcag aatgacatcg cacttacgaa
    10981 catggtactc aacccattcg cgattgatag tgatgtcgcc ttcaaagaaa tgcattctgg
    11041 gattgctgag caaatcaccg attcggtcgt tttgcatgtc catgccatag acctcccacg
    11101 aggtggtctc tagaatgcgc ttggatagat gatgaccaat aaaaccattc acacccaaaa
    11161 tgaggacttt tttcataaaa ctaattcctt ttgattgaac tacaccacta ggcattcatt
    11221 gaacttctac ggatagaagt tcaagtactt tgccatcacc acagaggccg aagtatcgat
    11281 tatcgactag atgaagtcct ggcgtcgatt ttttgaggat ctgagcaact tgggggctga
    11341 gatcggttcc aagattaagg ggtagtgagc ttttggaaag aatccatatc tttccttgaa
    11401 actcggtaaa tgcacctgga tagggcgggg caacggcccg aattaagttg taaattgtga
    11461 tggcaggctg agaccaatca atacggccat cctcaggttt gcggcccccg taataattgc
    11521 ctcggctgag atcatttggg cttcttggaa tctcaccctt cataagcttt ggtaacactc
    11581 tttctattac tcgcttagct gcattactta ccttattaaa tacatcatgg gcagtttcat
    11641 ggatctcaat agggactgca atttggtcaa cgatatcacc ggcatcaggt ttagcttcca
    11701 tcacgtgcaa agtagccccg gtttccactt ctccatgcag aacagcccaa ttaacggggg
    11761 cgcgcccacg atactttggt aatagagagc cgtgcatatt cagcgctgca agcttgggca
    11821 ctttcaataa ctctgccgga atcatgaagc ggtaatagaa ggaaaaaata tagtctggtg
    11881 atagcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnttggca acactgccaa accaaatatt ttcattgggg tcatcatcgt
    12001 gcgtaatgac tagatcgata tgcatccccg catcgagcag agcctgcaag cagtacacgc
    12061 ccacatcgtg gtaggcaaaa acaactgcat gtttattgac ttgattcaag aatctaggat
    12121 tttgcttgct ctagaacggc gctgaccatg tagcgtggcc gttgccgaac ttgttgatag
    12181 atgcgaccaa tgtactcacc aagcagtcca agcccaaaga gcatgacccc aataaggaag
    12241 aaagtaagcg caaacagggt aaagacaccc tcgacctcag cgcctaatac aaagcgccga
    12301 atcaagagta ataaaaatag agagcctgcc gctccagcca acaacatccc aaggatagaa
    12361 aaaatctgga gtggcatcac cgagaaacca gtgaccaaat caaaattaag ccggatgagt
    12421 tgatacaagc tatatttaga ttcccctgca aaacgttctt cgtgtttaac agagatctca
    12481 atcggcttat ttgcaaaggt gtaggcgagc gcaggaataa atgtattagc ttcctcgcac
    12541 tgtgatacca aatgaacaat gcgacgatgg taaccccgca tcatgcaacc ctgatcggtc
    12601 atggtgatcc gagtcatttt gtcacgcaga tgattcatgg cacgcgaggc actctttcga
    12661 aaccaagagt cttggcgatt ctcgcgaatg gtgccaacat aatcatgccc ctcctctagt
    12721 tgacgaacaa tttttctaat ttcttccgga ggattttgca aatcagcatc tagcgtaatg
    12781 acgtattcac cacgagcgta ttcaaagcca gccatgatcg ccatatgctg accaaaatta
    12841 ttagcaaaca gaactacacg agttacatca ggacgctttt catactgcat cgccaaaata
    12901 gctgcagagc gatctttgct tccatcgttc acaaaaacaa tttcatacga tagggcaaat
    12961 tgagaagtga tttgatcaag cgcgggatag agtcgatcaa acaaagcatt tagaccatct
    13021 tcctcgttgt agaccggaat gaccacgcta aggcgaggcg catcactgct aaattggatt
    13081 ggagagttgt tcatgtctac attttgcctg aacagcgact tgcgttatta gttcaggact
    13141 tgagtgaggg cctcgaccac gcgatcaata tcgtgtgagc tcatggtggg gaataaaggg
    13201 atggtcagga tagagcgacc aatctccgca gcaatggggg tggattcggg gcgatagcct
    13261 aatttacgat agagctcaaa cccagtaatg atggggtaat gcaccccagt accaatgcca
    13321 cgctctttca gggttgtcat catgtcacca cgagagcgtt gcaagcgagt caatggcaaa
    13381 gtaacctgaa acatatgcca gttactctgt tcatagtcag ctacgggcag acctaagcct
    13441 ttgttagcta aaccggcttg agtaattttc tggaaatatt ctttagctaa ctcgatacgc
    13501 ctcttctgaa attgatctaa ctgtttgatc tgatgcaagc caatcacggc attgatatcg
    13561 gtcaaattat ctttaccgcc caagatgtcc acttccatcc cgtcgggacc gctgcgaact
    13621 aaaccttgta atcgtaattt ctctgctaga cgtacttggt cttcgccaaa acgttcagta
    13681 ttaaaaacta aacaaccacc ttcaatggtg gtgaggtttt tatttgcctg aaaactaaaa
    13741 ctgactaaat catgtttacc tgcatgacca attcgggttc cattccagga agatcctaag
    13801 gcttgcgccg catcttcaat cacgcgcaaa ctgtgttgat ttgccaatgc atataaagca
    13861 tccacatcaa gcggcaagcc tgctaggtag actggcatga tagcttttgt atttttagta
    13921 atagccttag ctacttgatt gagatctaga ttacgtgttt gaggatcaac atcaacaaat
    13981 attggctttg cgccaacggc cagaatgaca ttggacgtgg ccacccaaga aatcggggtt
    14041 gtaattacct catcgcccgg accaatcccg gcaacctgaa gtgcaatctt cattgtggct
    14101 gtaccgtttg caaaacagcg cacagttgcg ccgcccagat aatctccgag agcattctca
    14161 aactctagaa ccttagggcc tgaggtaatc caacctgagc gtagaacatc agcaacggca
    14221 gcaatcgtcg cctcatcaat cgtgggtttg gtaaacggaa taaatggcag ggtgtcttga
    14281 ctcattcgtc tattgtcgca aagctggcga agggtgtcta acgattactc gtctggagtc
    14341 tctccccagc tcctccatgg gaagattcat ttgcttgagc tcctcatatt gagagggaat
    14401 catgactgcg attgctttgg ggtcttggtt ccagagggcg atgaactctt gtaaagaagg
    14461 aaccaattta tgaggctctt gtttggaacc aaaggtcagc tcatcggtaa attccaccat
    14521 gatggtatta cgctctaagt aaaacggtag ggtatggtca agcagacgca cggagtatat
    14581 cttggcatca ctaggtattt gacctttcac cttttgggca aggtcatagc cagataccgc
    14641 acgacctaga gtttcgtgac cggtgccggc gatcgttgct gttaggaaaa atccaaacgc
    14701 aaacaatcca atactgagca gggaatttct tttagcaagc agcgctgcac agaggctaaa
    14761 actgaacaag catagaagtg ctaccgtaat ccaaacggta taggcttgat atgcttcgac
    14821 ctcatcgggt tgaccgtttc gtccaacctc agacagaaag aaaaagccga cgattgccag
    14881 aactgcaaaa aagagtgttt gcactctcca gccattcgat agactggggt gggtttctaa
    14941 atagtcaatg atggatttgc ttgccaagat agcgagtgct ggaaataccg gcatgatgta
    15001 ccccggtaat ttagagcgtg acacgctaaa gaaaagcagg atgatgacaa accatgccca
    15061 gagcatccag cttgccgaga attgaccaag gcgatactgc ttgatagttt gccaagccga
    15121 ttggaagaac tgaggcatcc atggcagaaa accaattatc actaggggca agaagaagta
    15181 aaagggtgca gtacgccgat gcgcatcagc tgtgaagcgt tcaaagtgct catgaataaa
    15241 aaagaaatgc gcaaactctg gattttgtat cgatactgca ataaaccaag gtgcagtcac
    15301 tgctaggaaa atgattaagc cgctaacaat gtgaagccta gtcaaaatac gccaatccca
    15361 gcgagtgatc atgtatgcaa ataacaccat ccccgggatc acaacaccaa tgaggccttt
    15421 tgagagggtc gctagaccca ttgctaacca acaaatccac atccaatgtc gcgctgcgcg
    15481 cagacgattc tcattaaaac tatgttgagc cagaagtaat gaacatagcg ccagattcaa
    15541 aaaggcagac agtcccatat cgagagcatt aaagtgccca cccaccaccc acattggact
    15601 tgccaataag atgagtgctg caagatagcc tgcgagtgat ccatacagac gaaatgcagt
    15661 aaagcccacc aacagaatgg tgaagtagct agtgatgcca ctccagaatc gagcctgcca
    15721 ctctcctaaa ccaaataatt gaaaggtgat tgcagtaacc cagatgtgaa agggcggctt
    15781 ctcaaaatac ttatagtcgt tatagcgggg cgtaatgtag tcaccgctca ccatcatttc
    15841 acgagccatc tgtgcatagc gaccctcatc gcttgggatg aggtggcggt aatcaagtgt
    15901 gccaaaccaa agcaaagtag ctaaagtaaa taatagcaac agtgctgggg agcttaaggg
    15961 cgtaatttgg cgattggcgt tcatgagagt ccgtgttaaa ccaaattgct tgtatctaga
    16021 atgatggctg ctaatacctg acgaccctcg ttcatcaaaa tgttgtacgt gcgacaggct
    16081 gcttgggagt ccatcatttc aaatccaatt ttggcttgta tgaggggctt aattaactcc
    16141 ggtttcaaga attgctgttt cttacccgta ccaatgatga tgagctctgg cttaaatacg
    16201 cataagctgg ctaaattatc ggacgataga tccgcaaacg cacttacggg ccaggggctt
    16261 atttcaccct ctggttggac aattagcgca tgtggataga cctttgagtt gacctcgata
    16321 tagccaattc cgtagcctgt gattgtattt aggctggatt gggggtcaga atgtaacttc
    16381 acgccgaggc tctgtcataa ttaagggatt atagctagca atttatccct agatttcaag
    16441 agcttaacca tttagaagac ctcattgtga aaccgatcca aaagtccgac aaactaaata
    16501 atgtctgtta tgacattcgt ggccccgttc tggagcttgc tcagcgcatg gaggaagagg
    16561 gtcagaagat tattaagctc aatattggga acgtcggtgt atttggtttt gacccgcccg
    16621 aagagattca gttggacatg attcgtaatt taggtaatgc ctcagcctat tctgactcaa
    16681 aaggtatttt tgcagctcgc aaagcaatca tgcagtactg ccaagagaag ggtattcagg
    16741 gcgttaccct tgacgatatt tataccggta acggcgcctc tgaattaatt gtgttggcca
    16801 tgaacgcatt actaaataat ggcgatgaag tgctcgtgcc ggcacccgac tatccattat
    16861 ggacggctgc tgtgtcttta tcgggaggca cccctcatca ttacttgtgt gatgaaacta
    16921 aagactgggc gcctgatatg aataatctgc gctcaaagat tacgccgcgc accaaggcaa
    16981 ttgttgtcat caatcccaat aatccaacgg gtgctttgta ttccaaagac attttgttgg
    17041 aaatgattgc agttgcacgt caacataatt taattctgtt tgttgatgag atttatgaca
    17101 agatgttgtt tgatggtgag aaacatattt ctctggcatc gctctcgacc gatgtcgtca
    17161 ccattacctt taacggactc tccaaaaact accgttcgtg cgggtatcgc tctggctgga
    17221 tggtggtctc gggtgataag gcgatggtcg ctgactatat tgaagggctc aatatgttgt
    17281 cttccatgcg tctatgtgca aacgtacctg gacaatatgc gattcagacc gcattagggg
    17341 gttatcaaag tattaatgac ttggttgcca aggacgggcg gctcgcaaga cagcgtgatc
    17401 ttgcgtataa attaatgagc gagataccgg gtgtaacggt tactaagcca aaggctgctt
    17461 tatatctgtt tccaaaatta gatccagata tgtaccccat taaggacgat cagcaattta
    17521 ttgctgattt actgaaagag gagaaagtac tgctagttca aggcactgga tttaattggc
    17581 cacacccaga tcatttccgg ataacgttct tgcctcatga agatacctta cgcgaggcaa
    17641 ttaagcgtat tgcccatttc ttagaacggt atcgtaataa acacgcatta aaaaagggtg
    17701 ctgcggcgac agcaaaagca taatgaaacc cattcaagta ggacttctcg gtatcggaac
    17761 cgttggtagc ggagtattta ctgttttagc tcgcaaccaa gaagaaatta aacgccgcgc
    17821 tggccgcggt attcggattc atatggttgc cgatctcgat accaagcgtg cccaagaaat
    17881 ggtgaacggt caggctgagg tggtggctga cgcgcggaag gtgattcaga accctgaaat
    17941 tgatatcgtg gttgagttaa ttggtgggta tggaattgcc aaggatctag tgcttgaagc
    18001 tattgctgcc ggcaagcatg tcgtcactgc gaataaagcc ttaatcgctg ttcatggtaa
    18061 tgagatcttt aaggcagccc acgccaaagg ggtgatggtt gcctttgaag cagcggttgc
    18121 tggtggcatc cccatcatta aagcattacg tgagggctta agtgctaacc gaatcgagtg
    18181 gattgcgggc atcattaatg gcactactaa ctttattttg tccgagatgc gcgataaagg
    18241 cttggacttt aatactgtct taaaagaggc tcagcgcttg ggctatgccg aggcagatcc
    18301 tacttttgat attgaaggta ttgatgctgc ccataaggcc acgatcatga gtgcgattgc
    18361 atttggtgta ccaatgcaat ttgcaaaggc ccatgtggaa ggaattacca agctttctgc
    18421 aatcgacatt aaatatgcag aacaattggg gtaccgtatt aagttactcg gtattaccaa
    18481 gaaggtccca agtggtattg agttacgtgt gcacccaacc ttaattccta caaagcgctt
    18541 attggctaat gtggaaggtg cgatgaatgc cgttcaagtt cacggcgatg cggttggcac
    18601 tactttgtat tacggtaaag gtgctggctc tgagccaact gcatccgcag tgatcgcaga
    18661 tttggtcgat gtgacacgcc tacaaactgc cgatccagaa aatcgggtcc cgcatttggc
    18721 atttcagccc gatgcattac aaaacacgcc gattttgcca atggcagagg tcactacaag
    18781 ttattactta cgttttgcgg tggcagacca ggctggtgtt ttggctgaga taacacgtat
    18841 tttggctgct catggggtat ccattgatgc cctgttacag aaagaggccg cagagggtga
    18901 gagccaaacg gatctggtga ttttgaccca cgagaccaaa gaaaaaaata tgatggcagc
    18961 aatcacagag atgcaagctc tcaagactgt gctcggcgag attatcaaga ttcgccttga
    19021 aaacctctcc taattagaga gacttatatg cgttatcagt cgactcgtgg tgccagtcct
    19081 gtgcaatcgt ttcagggaat acttttgggg ggcttggccc cagatggcgg actgtattta
    19141 cctaatcagt atcccctggt aacaacacag caactcgatt catggcgggg tctatcgtac
    19201 gccgaccttg cctttgagat tttgagtctc tattgcgatg acatcccagc agaggattta
    19261 aaaaccttat tgcataaaac ctatacccct caggtttact gtaatgggcg agcaagcgat
    19321 cgggcgagtg atatcacccc catccattgg ttaggcgaag aaaagggtgc aaagcttgga
    19381 ctgctttgtt tatccaatgg cccaaccttg gcgtttaaag atatggcgat gcaattgctg
    19441 ggtaatttat ttgagtacgc tctaaagcgc gccaatcaag acctaaatat tttaggggcc
    19501 acttcgggtg acacaggcag tgccgcagag tatgcaatgc ggggcaagca tggcataaaa
    19561 gtatttatgc tctccccacg cggcaagatg agcccatttc agtctgcaca aatgtattcc
    19621 ttgcaagacc ccaatatatt caacttggct gttgaaggtg tatttgacga ttgccaagat
    19681 atcgtgaagg cagtaagcaa tgatttggtg tttaaagcca aataccgtat cggcacagtg
    19741 aactcgatta attggggtcg cgtactggct caagtggttt attactttca gggatatctc
    19801 ctagctaccc aatcaagcgc cgagaaggtc tcctttgctg taccctcagg aaactttggc
    19861 aatgtctgcg ccggccacat tgcacggatg atgggcttac ccattgagcg attagtagtt
    19921 gccaccaatg aaaacgatgt gctcgatgag ttctttaaga ccggtatcta ccgcgctcgt
    19981 aaatctgccg agactttaca tacctccagc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tgatttgtcg aaggatgtgg cttttcaaag
    20101 gattcagcaa tttggttttg tctcgggtcg gagtacacat caaaaccgtt tagaaaccat
    20161 tcgtcagatt gatcagcaat atggggtcat gattgatacg catacagcag atggtgttca
    20221 ggtggccaga cattatttgc aaaaggatgt gccgatgctc gttttagaaa cggccttgcc
    20281 tattaaattt gaagagacca ttcaggaagc cttgggacgt cctgctgagt gccccagtgc
    20341 tttcaaagac attaagacaa agccacaacg ggttatgaac atgccagccg atacgatgca
    20401 gataaagcag tttattgaac aacacgtcat ttaataaatt taagtacgat gcaatccact
    20461 ccaagtaagc caccaatgct cagtgcggta gaggcccttg cccatttatt aaaaaatgca
    20521 agtcctgtca acgaggaaga aacggttcca acgcaggcag ccttagggcg tgtgctcgcg
    20581 aaggatatcg ctagccaggt tgatgttcct ccattggata acacctctat ggatggctat
    20641 gcggtgcgtt cttcggaaat tacgcaggtt ggaaccgtgc tacggatggc ccagcgtatt
    20701 cctgctggat cggtaggaca aagcctggag acagggacca ttgcccgaat ttttacaggc
    20761 gcacctattc ctcagggagc cgatgcagtg gtgatgcaag aagattgcat cgttcaagat
    20821 caaaacgaat tagtacaaat caatagcgtt ccaaatgctg gtcagtggat tcgtcgacgc
    20881 ggcgaggatt tgaccgcggg caagcttgct ttaacagctg gaagcttact gaggcctcag
    20941 cagttaggcg ttgctgcctc ggccggttat gcacaattac cagtaaaacg caaagtacgg
    21001 gtcgctgcct tttttactgg agacgaactc tctttgcccg gtgagccatt aaaaccaggc
    21061 ggtatttata actccaatcg ggatacctta ttaggagtga ttcggagttt gggttgtgag
    21121 gcgacggatt acggaattgt ccccgatcgc ctcgaggcaa cccgcgcagc tttgcgcact
    21181 gccagtgcca atcatgattt gatcattact tcaggagggg tctcagtagg cgaagaggat
    21241 catattaagc ctgcagtgaa tgcagaaggt cgtttagatc tctggcagat tgggattaaa
    21301 cctggtaagc cgctggcttt tggagcagtt cgtaaacagc aacagagcga tcaggagact
    21361 tggtttattg gattaccagg aaatcccgta tcgagttttg taaccttcct gctgtttgta
    21421 cggccgttta tcttaaaact acaaggccgc tcagatnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaccaaag ctccggggtc ttaaccagtg
    21601 ctgcttgggg cgatggtttg ctggattgtc ctgctggtca gggatttaaa aaaggtgata
    21661 tggtgcgcta tatccctttt agtggacttc taagttaatt aagcaaagat gaagctacac
    21721 ctacgttttt ttgcctctat tcgagaatca ctcggtcttg cgcacgagga tattgatcta
    21781 ccagaacata ttaagaccat tgatgatctg cgtacccatc tcattgcacg gggtcatccg
    21841 tgggctgaga ccttggccaa ggataaggtc ttgcgctgtg ctctcaatca acaaatggtg
    21901 agcgctgatg cgaccttgat agaggatgct gaagtcgctt tctttcctcc agttaccggg
    21961 ggctaagatg ccagttcgaa tccaagaaca tgactttgat ctaacgcaag agattgctct
    22021 tttgcgtaag gatgagccgc aaatcggggc agttgctatt tttattggaa ctgttcgcga
    22081 cctcaatgaa ggtagtcaag taaaagccat gaccctggag cactatccgg gtatgaccga
    22141 aaaagccctg cacgatattg ttgatgaagc aaagaagcgc tgggacctaa aggatgcttt
    22201 ggtaattcat cgagtgggcc ctttgatgcc cctggatcaa attgtgttgg tggcggttac
    22261 gagcgcgcat cggggcgagg catttgcagc ctgtgagttc atcatggatt atctaaaaac
    22321 attagccccc ttttggaaga aagaggatac gccacaagga gctcgctggg ttgacgcgag
    22381 ggtctcggac gatcaagcga tggagcgttg ggctaaaaag taatccggcg cgatttgggt
    22441 taatcaaaga aatccagttt tttgtgacgc ggcatccgtt taatcagagc ctctgctttg
    22501 gaggcgcttg agcgatccaa atattccttg ctggcgagga gtttgaccgg acccctgccg
    22561 cgggtataac gtgccccaag accagagtta tgagcgttaa tccgctctgg caggcggttg
    22621 gtaataccag catagaaact gccatcactg cattcgagaa gataaagaaa ccaagccata
    22681 atttgtttta aatcaagtga atacttgaat atatgctatt catccccata ttctaggtag
    22741 attagtaatt ttgcaaataa attgagataa atcatgcgta tagataaatt aacgacgaag
    22801 tttcaggagg cgctaggaga tgcccaaagc cttgctttag ctaaagataa ccagttcatt
    22861 gagccagaac atttgctgtt agcgatgtta cggcagcaag atggcggtac ccgtagcctc
    22921 ttaactcggg ctggggtaaa cgtcactggg cttgagaagc ttgccgaggc aatgattaat
    22981 aaattacctc aagtgcaagg gactggcggt gatgtgcaaa tagggcgcac actactcggc
    23041 tggcttaatc agacagaaaa agaggctagc aagcgtggcg atcaatacat tgcgggcgaa
    23101 ctcttcttgc tggttttggc tgacgataag ggcgagctgg gtaaggctgc gcgtgagaat
    23161 ggcttaagtc gcaaagcact ggaggcagcc attgatttag ttcggggcgg tgaggctgtg
    23221 aactcagcag atgcggaagg tcagcgcgag gctctaaaaa aatacacact ggatctcact
    23281 gagcgtgccc gcttgggcaa actagatcct gtcattgggc gagatgatga aattcgtcgc
    23341 acgatacaga ttctgcaacg ccgaaccaaa aataacccag ttttaattgg ggagccagga
    23401 gttggtaaaa cagctattgt ggagggtctg gcgcaacgta ttgtgaacgg cgaagttcca
    23461 gaaacactga aagataagcg tgttttagtg cttgatatgg ccttgttgct tgctggtgct
    23521 aaatatcgtg gtgagtttga ggaacgtctg aaggcagtct tgaacgacat tgccaaggat
    23581 gcaggccgca ccattatctt tatcgatgag atccatacga tggtgggtgc cggtaaggcg
    23641 gatggcgcaa tggatgccgg caatatgctt aagcccgctt tagctcgtgg agagcttcac
    23701 tgtattggcg caactacctt agatgaatat cgtaaataca ttgagaaaga tgctgcattg
    23761 gaacgtcgtt tccaaaaggt catggttgat gagcccagcg ttgaggcaac gattgccatc
    23821 ttacgtggtc tgcaagaaaa atatgagctg catcacggta ttgaaattac agatccagcg
    23881 attgtggccg cagccgagct atcacatcgc tacatcacag atcgtttttt gccagataag
    23941 gctattgatc taattgatga ggctggatca cgcatcaaga tggagattga ttccaaaccc
    24001 gaggtgatgg ataaattaga tcgtcgtctg attcagctca agattgagcg cgaagcagtc
    24061 aagaaagaaa aggatgacgc ttctaaaaaa cgccttgagt taattgagga agaaatcaag
    24121 cgcttaggtg cagagtatgc tgatctagaa gaaatctgga aagcagaaaa gggcgcggct
    24181 ctgggtgccg catcggtcaa agaagagatt gagcgagtaa aggcagagat tactaaactg
    24241 cagcgcgagg gtaagcttga gaaggtggca gaacttcaat atggcaagtt acctgaacta
    24301 gaggcgaagt taaagtcagc tgctgccgct gaagcgaaag cccacaacaa ggatggtcat
    24361 cgccctagac tattacgtac ccaagtgggc gcggaggaga ttgctgaagt ggtctcgcgg
    24421 gcaacgggta ttcctgtatc caaaatgatg caaggagagc gcgataaatt attgaagatg
    24481 gaggaccact tgcacgagcg agtgattggt caagacgaag caattcgtgc agtgtcggat
    24541 gcgattcgtc gctcgcgcgc cggactctct gaagaaaaca agccttatgg ttcgtttatg
    24601 ttcttgggac caaccggtgt tggcaagact gagttatgta aagcccttgc cgaatttctg
    24661 tttgatagcg atgatcgctt gattcggatt gatatgagcg agtttatgga aaagcatagc
    24721 gtggctcgcc tgattggggc gcccccaggt tatgttggct atgaagaggg cggttatctc
    24781 acagagcaag taaggcgcaa gccctatagc gttattttgt ttgatgagat tgagaaagcc
    24841 caccccgatg ttttcaatgt tcttttacaa gtgctagatg atggcagatt aactgatggc
    24901 cagggccgta cggtcgactt taaaaacaca gtgatcgtta tgaccagtaa tattggttcg
    24961 catttaattc agtcgatgac cggtaagaat caatctgaag ttaaggatgc tgtctttgga
    25021 gagctaaaga atcatttccg cccagagttt ttaaatcggg tggatgagat tgtggttttc
    25081 catggcttgg atcaaaagaa cattgcggcc atcgcaaaga tcttgttaca aaacttgtct
    25141 gaacgtttgg ctcgcattga tatgcaactt gatgtcagtg atgcggcgtt gagtaagatc
    25201 gccgaagtag gctttgatcc tgtatacgga gctagaccat taaagcgtgc gattcaacag
    25261 catattgaga accccgtttc caagatgatc ttggagggta agtttgggcc gcaggatgtc
    25321 ataccggtcg atgtggataa aaagggtgac tttagcttct cccgcttagt acattagttc
    25381 ttttctggag taattcccga ctggcgctaa actagtgcca tgtcggtagc tcaaggtagc
    25441 ttgaacccca gtgacaatcg gacgataggc ctaatcagcc tagcgcatgg tacctcgcat
    25501 ttttatcacc tcgtattgcc accacttttt ccttggctaa aaatcgagtt cgctctttca
    25561 tacgcagagc ttggattgtt gatgaccatc ttctttgtgg tctcctgcgt tgtgcaggcc
    25621 tcctcaggat ttttggtaga tcacaagggc gctcgcccag ttttatttgc tggcgttggc
    25681 ttattagcta tgtccgcatt gacctatgca gtgagctcga gttatgttgg tttagtgatt
    25741 ggggcggtct tgatgggttg cggtaatggt gttttccatc ccgtggatta caccctcatg
    25801 aaccataaga tctcgcccaa gcgtttgccg tatgcgtatt ccttccatgg tgtaacgggt
    25861 tatcttggtt gggcctttgc cccagtcttt atggtnnnnn nnnnnnnnnn gatgacgcca
    25921 aggcccgcca tgaatccaat ctagctaatg cacgtaagaa cgatcccaat cacaccccca
    25981 taggtacatt tgattttcta aaattaccgg ctgtttggtt gtgctggata tttttcttct
    26041 taagcatggt agcaatggct ggcattcaat cgtttgctcc cagtgctctt ttaaagatat
    26101 acgatatttc agtggcatca ggtaatttct tcctcaccct attggcctta ggtagtgcgg
    26161 ggggtatgat tttaggaggg tatattgcta gtaagctagc ttcgccagag cgtaccgtgg
    26221 ttatttgttt aatgattagc gccttcatgg ctgtcatcat tggtacggag tttgcacccg
    26281 ctgcgttcat acctagtcta tttgtagtta ttggcattgg tcttggagtt gccgccccct
    26341 cacgtgattt aatgattcgt tcggcaacgc cgcagggtgc ctccggacgt gtatatggca
    26401 ttgtttattc cggcattgat ctcggcgcat cagtcggccc cttagccttt ggaatcttgc
    26461 ttgatgcagg ccaaccgaac tggctctttt taggcgttgc ctttattcaa tgcctcatta
    26521 tcttcacggc atttaaggtg gccagcaata tcccaagcaa ggctcctgct taagtgcgtc
    26581 ctgttcaggg cgaaatactg gagatttggc cacagcatct ctttccgtat tgtgaaatat
    26641 tgattccaca agataaaacc actccaaacc atacttcata aacctttttc attctctaat
    26701 cattgttttc gtattgtgat aataagaata caactaattg atttaaatga ataatattta
    26761 tttgtttcac ttttgacaaa taggctcagt ctttgtattc ctccttaaac tcttatataa
    26821 gacataagag ttgatacgaa caaatgttca attcataaga gtttttatct tgatagaagg
    26881 agagcaatat ggtgacccgt aaggcagagg cagcaacaat acaaaaggat tgggatacca
    26941 atccacgttg gcagggtatc aagcgtggct atagcgcaga ggatgtggtg cgcttgcgtg
    27001 gctcagtgcg acccgagtac acccttgcac gacaaggcgc cgataagtta tggaatcttg
    27061 tcaataatga ggcatatgta aactgccttg gcgcattaac tggtggacag gcaatgcaac
    27121 aagttaaggc cggtgtaaaa gccatttatt tatcgggatg gcaagtagcc gctgataaca
    27181 atagttatgc ggcgatgtat ccagaccaat ctttgtatcc agtggactct gttcctaagg
    27241 ttgtggagag gatcaataac acttttgagc gagccgatca aattcagtgg tctaaaggta
    27301 ttaacccagg tgatcctgga tacattgatt actttgcgcc gattgtggct gatgcagaag
    27361 ctggattcgg tggtgtatta aatgcttacg agctcagtaa agcattaatt aaaaatggtg
    27421 ctgcaggcgt ccactttgaa gatcaattgt cttctgtaaa aaagtgcggc catctcggcg
    27481 gcaaggtctt attaccaacc caagagtccg tgcagaaatt gattgcagct cgtctagcag
    27541 cagacgttat gggcgtgcca acgatcatct tggcaagaac ggatgcggag gctgctgatt
    27601 taatcacctc tgattatgat gagaatgaca agccattctt aaccggtgag cgtaccccag
    27661 agggctttta taagacccgc aaaggattag ggcaagctat ctcgcgtggt ttagcctatg
    27721 cccaatacgc cgatatggtg tggtgcgaaa cgggtacgcc tgatctcgag tttgccagga
    27781 aatttgcaga agcgattcgg gagaagttcc ccggcaagat gttggcatat aactgttcgc
    27841 catcgtttaa ctggaagaaa aatttggatg acaccacgat tgctaagttt caacgcgagc
    27901 ttggcgcaat gggctataag tatcagttca ttaccttggc gggcattcat tccatgtggt
    27961 acaacatgtt tgacctctca caagattacg tcaagcgcgg tatgaccgct tatgttgaga
    28021 aagtacaaga acccgagttt gcagcgcgcg atcgtggcta tagctttgta tcgcatcagc
    28081 aagaggttgg cacaggttac tttgatgatg taaccactgt cattcagggc ggtaagtcat
    28141 cggtaacggc attaaccgga tctaccgaag aagagcagtt tcactaaagc catcactcgc
    28201 caatccggcg aataggcggg cagtaaggga agcacttaaa ttattccaaa aggatgactt
    28261 aagtgctttt ttgttttaca ttaaaagcat gagcactaat tgcattttat gtaaggagga
    28321 tggtggccaa ctaatatggc gcggggatga tgcgcgcgtt gtcttgattg atgatcccga
    28381 ccttccaggg tattgccgta ttatctggaa tcgacatgtt gcagaaatga gtgatctctc
    28441 acaagtagag cgagagattt tgatggcctt ggtggatgtt accgagtttg cggttcgtcg
    28501 agtgatgcat gctcagaaga tcaatattgc ttcgttaggt aaccaagtac ctcatctgca
    28561 ttggcatgta atacctcgtt ttgtagatga tccatttttc ccgggatcca tttggtcaag
    28621 taaacagcgc gagctctccg ataccacaag aaaagcaaga cgagaagctg ctaaaggttt
    28681 gcctgatgct atacgctctg gtatagccga tttagcctag agtaatttct gtaaatattc
    28741 taaataattt tttatgtcaa gtgcgctcgc gtcacgctgt gcctgccaca aaacttgccc
    28801 taggcactcc ataatttgat gttgtgcttc gtgttcagaa tccaatttcc tactcaatgt
    28861 atttnngtaa attcttgctc gagggcgcct gatggatcac tcaagatttt atgaaactct
    28921 gggtgctgct ccatccactg actggcaatc atctctagtg gagttaaaac atgaccatct
    28981 tgatactttt gccaggttgt gcaaaagaat tctcggacct cctcacggga tggattaaat
    29041 agggccacgt acttttccag tgccagtttt ctgtttatac gaacaaaggg gctcaacggc
    29101 gcaatgtgca caatcgggat tgcgggcttt gcagatatag cgaccatgca aaatcagcca
    29161 atgatgggca tcgtgtaaaa attcttgcgg aacacgtttc attaatttct tttcaacctc
    29221 cattacattc ttgcctggcg ctaaacctgt ccggttagag actctaaaaa tatgagtatc
    29281 gacagcaatg gtggcctcac caaacgcggt atttagaatg acattggcgg tcttacgacc
    29341 aactcccgga agagcctcta gatcttctct ttttttagga acttcaccac catgctgctt
    29401 cattaatatg cgacaggttt cttgtagatg ccgcccctta gttttataca ggccaatatg
    29461 ttgaatataa ggtcgaacac ctgcctctcc aagagccaga agggctgctg gggtattcgc
    29521 tattgggaag agccgtcgtg tccctttgtt tacggagata tcggtcgctt gtgcggataa
    29581 aagaacggct gttaatagct caaatgggga gctgtattcc agttcggtac tcggttttgg
    29641 gttattggct tttagctgtt caaaaaaagc aatgcgttgt tcggcattca tagaaagact
    29701 attagtttga atgtttgacg cgcgctatag cggcagcaat gatggcgcgc ttacgctcaa
    29761 tctcatctag ttcggtttgg gagccggctt gactctcgtc cagcgctgcg agttttgcct
    29821 ttgccttctt agcaagacgc aactcattat cgcgctcttc acgttctagc cgttcttggt
    29881 gcgtttggta acgcgctctc gcaagatctg cttgagagct agaccatgca tcccaccctg
    29941 tttgatcatt tgtggtgttg atcatggtga tacagtccac agggcaagga gcaacacata
    30001 gatcgcaacc agtacaccat tctggtagga ccgtatgcat ttgtttagaa gcgcccacga
    30061 tcgcatcaac tgggcatgct tggatacata gggtgcaacc aatacatgct ttggggtcaa
    30121 tcaaagcaac cggtctggga cgttcgatgc cacattctgg attaatctgc ttcgtttttt
    30181 cattgagctt gaactctaaa atctggctga gccgaatgat gccctcttgc ccgcctggag
    30241 gacagcgatt aggaagttcg ccattggcca ttgcttcggc ataagctcgg caatctggat
    30301 agccacactt cgtgcactgc gtttgaggta atgtgtcctc taataagtca gctagcttgg
    30361 tagagcgcat tgacttcgat agaaactgac ttaattaggg tcttgattat ttggactgtt
    30421 agcatggtga gcgcgtataa agctctgtat ctgcggatac accttattac gccagcgtct
    30481 tccagaaaag atgccatagt gcccagcgcc gcttacctcg taatgctgtt tctttgactc
    30541 atctatccct ttgcagagtt tgtgagcagc gcgggtttgc ccgcttccag aaatgtcatc
    30601 catttcaccc tcaatggtga gtagggcaga ttgttgaata tcgtgcggtt tcacaagttc
    30661 gtcattcacc atccaagtgc cattgggtaa tgcgcactct tggaatacag ttctgatggt
    30721 gtccagatag tagtgtgcat ccatgtcaag cacagcgttg tactcatcat agaaacgaat
    30781 atgcgctttg gcatcttgct catcaccccg tactaagttt tggaagaaat cccaatgaga
    30841 ctgcatatga ttgctggggt tcatagcaat aaaaccaagg tgctgtaaga agccaggata
    30901 aacacggcga cccacgccag gatggggtgg cggaacgtta tgtatcgtat tggcttcaaa
    30961 ccattgaatc gatttatttg tggccaagga gttgacggct gtaggatgtt tgcgggcatc
    31021 aatcgggccg cccatcatga ttagtgattt aggagttgcc tcgccccgtg aagccattaa
    31081 ggaaactgct cccagcaccg gtacggtagg ttggcataca gaaatcacat gaagattatt
    31141 agcgccaatc aagcgaatga aatcttcaat gtaattaaca tagtcactta atccaaacac
    31201 cccttggtca tttggaacca tccgcgcatc cacccagtca gtgatataga ccttgtggtc
    31261 ttgcaacatt gtgcgcacag tatcgcgtaa taaagtggaa tgatgacccg agagaggggc
    31321 aacaattaac acaatcggat cttgcttcat tttggtgatg acttcgacat catcagagaa
    31381 gcgcttgaag cgaacgaggt tacaaaatgg cttcgaaagg gctgtgatct cttgaaccac
    31441 tacatcttta ccgtgggctt tgacgctgcg tattccaaac tcgggttttt tgtaatcttt
    31501 acccagacgg tgtaagagtt cgtagctagc tgcaaagcgt tctgctcctg gcaacaagga
    31561 caaagggttt gctgggttga tgaaagtctc agaggtggtt ttagcccaag atgttagggg
    31621 ttgtaacagc gctttctgaa aatcgtatat ttgataaagc attgattacc ctccagatac
    31681 agcaaaaacc ctattttgac acgagaaccc gagcgatagc ctgtgcaacc cgatctaaat
    31741 ttttggtatt caaggctgcc acacagatgc gcccagtcga aagtgcgtag ataccatcat
    31801 gctcttgcag tttggcaact tggtcagcag tgaggccgga ataggaaaac atgccacgct
    31861 gggcttcaat aaatgagaaa tcctgttgca caccagaagc cgctaatttt tggttgaggc
    31921 catggcgcat ggctttaatg cgctcacgca tctctgcgag ttcgtcttcc cacatctgcc
    31981 gtaacgtagg ggagttcaga acagtcgcca caatggcggc cccatgagtc ggcggattag
    32041 agtaattggt acgaatgaca cgctttagct gtgataacac ccgagtagat tcttccttgc
    32101 tttgagtcac gatcgaaatt gcgccaacac gttcaccata gagtgagaat gattttgaga
    32161 atgagctaga cacgaagaaa gacattccgg agtccgcaaa catgcgaact gcagcgccat
    32221 cttgctcaat accgctagca aaaccttgat aggcaatatc aagaaaagga attaactgtc
    32281 gagtttggca aatagaaata atttttttcc attgctctgc tgtgagatct gcgccggttg
    32341 ggttatgaca gcaagcatgc agaatcacta atgtttttgc gggaaaggac tctaaagatt
    32401 taaccatgcc atcaaaatca acgccgcggg tcttggggtc aaagtaggtg tactccaaga
    32461 cctgatagcc ggcggattca aaaatacctc gatggttttc ccaactcggc gcgctgatcg
    32521 cagccggtgc accagtatca agacgtttca caaagtcacc gcctacgcgc agcgccccag
    32581 ttccgcccag acactctgca gtcactacac ggccctcttt aatcaggggg gagtctgcac
    32641 caaacagaag gttttgcaca gctgagttat agggatttgg accttcgatc ggaatgtagg
    32701 cgcggggcga ttgtttggca accacctctt gttctgctgc caataccgcc tttagtaagg
    32761 gcaccttacc gtcgtcggtg taatagacac caactcctaa attcacttta cctgggcgct
    32821 gatcggcagt gtaggcttca gtgaggccaa aaatagggtc tttaggggct aattggacgg
    32881 aagcaaacag gttcattttt attgaattta gtttatttgt tgtaaatcag gttcttagga
    32941 atgattggtg caattggaat aaaaacggca aaattaacga ttgttggaaa tttgctgtgc
    33001 gattaagttc acagctataa atgatagccg agatgagtaa gcccacccca aaattagcca
    33061 atgccagccc taaaaataaa acggctgtgg ctggttcaaa aaaaacagcc aaggaagagg
    33121 gcgccatcct taatcggggt atacaagacc ctccccttat tgagtctcaa tttatacgtt
    33181 tcccagactc cccctatcag ttgtatcaac cctttcctcc agcgggtgat cagcccgcag
    33241 cgattgatca gttgtgtgcg ggtattgatg atggcttatc gtttcagacc cttttggggg
    33301 tgacgggctc tgggaagacc ttcaccatgg ctaatgtgat tgcgcgaatg ggccgacccg
    33361 ccatcgtgtt tgcacctaac aaaacattag ccgcacagtt gtactcggag tttagggagt
    33421 ttttcccccg caatgcagtg gagtacttcg tgagttatta cgactactac cagcccgaag
    33481 cttacgtccc gacgcgcgac ctatttattg agaaagactc ttcgatcaat gaacacatcg
    33541 agcaaatgcg tttgtctgct actaagagct tattggagcg tcgcgatgtg gtgattgtgg
    33601 caacggtctc ggcgatctac ggtatcggta acccaggtga ctatcacagc atggtcttaa
    33661 cactcaggcc cggcgacaag ttgacgcagc gtgatgtcgt cacacgttta atttcgatgc
    33721 aatacgagcg caatgaactc gattttcatc ggggtatctt tcgagttcgg ggcgacacga
    33781 ttgatatctt tccggccgag cataatgaat tagccattcg ggtcgagcta tttgatgatc
    33841 aagtagagtc cttgcatttc tttgatccac taaccggtaa gattaaacag aagttacctc
    33901 gcttcacggt ttaccccagt tctcactatg taacccctcg tgaaacgatt ctacgagcga
    33961 ttgagactat taagattgag ttgcgcgaac gccttgatga gtttgtcaaa ggctctaaat
    34021 tagtagaggc gcagcgccta gagcagcgca ctcgctttga tctagagatg ctctccgagc
    34081 taggcttttg taaaggaatt gagaattatt cacggcattt atccggtgca aaaccgggtg
    34141 aggcgccacc caccttggtt gattatttac cacccgatgc cctgatgttt ttggatgaga
    34201 gccacgtctt gattggacag ctcaatggca tgtataacgg agatcgctcg cgcaaacaca
    34261 ccttggttga atatggattt cgtttgccat ccgcaatgga taatcggcca ctgaagttcc
    34321 ctgaatttga gaccaaaatg cgccaagcca tatttgtgtc agcaacccca gccgattatg
    34381 agaaccaaaa aacggggcag gtggtcgagc aattggtccg ccctacgggt ttagtggacc
    34441 ctgccattga ggtattgcca gcgagcactc aggtcgatga tcttttgaat caaatccacg
    34501 aacgagtgaa ggtgagtgaa cgggttttgg taacggtttt aaccaagcgc atggccgagc
    34561 aattaaccga gtatctctcg gataatgggg tgaaggtccg gtatgttcac tcggatattg
    34621 atacggttga acgcgttgag atcattcgtg acctgcgact tggggtgttt gatgtgttgg
    34681 ttggcattaa cttattacgc gagggtctcg atattccgga agtgtcttta gttgctattt
    34741 tggatgctga caaagagggc ttcttgcggg ctgaacgcag tttgattcaa acgattgggc
    34801 gcgctgctcg aaacgtcaac ggcaaagcga ttttgtatgc cgaccgaatc accgactcga
    34861 tgaagcgcgc catgggtgag accgagcgcc gcagaaccaa gcaagttgcc tttaatctcg
    34921 aacacggcat tgagcccaag ggggttcgca agcggattaa agacatcatt gatggggtgt
    34981 atgacgtaca agagaagcgc ggtgagatgc aagttgctca aaatcgtgct cattacgaaa
    35041 gcatgagcga gaaggagctc gccaaagaaa ttggtcgcct tgagaagcaa atgatggcag
    35101 aggccaaaaa tctggaattt gagaaagcgg cagccactcg agaccaaatt gcgaaaatta
    35161 aggaactcgc ctttggtgct ttagccaaag actccctcta agggcctctg ggcttgcttt
    35221 tgccctagac ttaacaaccc ctttccctta ttcaccttaa ggtttattcc cgataaaaat
    35281 agtcgggaat atgttaaact tgaccgcaat tgtcaggtat taccctgctg acagttgttg
    35341 tccataacaa cccacaaccg aggtgatcta tgagacttac tactaaaggt cgatttgcag
    35401 taaccgcaat gattgatttg gcgttgcgtg aaacccatgg tcctgtaacg ctggctggaa
    35461 ttagtcagcg ccaaaagatt tctctatcgt atttagagca actttttggt aagttacgcc
    35521 gttttaatat tgttgagagc acccgcggtc ctggcggcgg ctacacactt gcgcgtaagg
    35581 ctgaggagat cagtgttgcc gatattattg tggccgtgga tgagccctta gatgcgaccc
    35641 agtgcggcgg caagggaaac tgcaataacg aagaaagcac ccaaagtcgt tgtatgacgc
    35701 atgacttatg gaccaatttg aactccaaga tggtcgagta ccttgattca gtgagcttac
    35761 gcgatctagc caagcagcaa gagagtcgtg gcgtagtgat tcaggatctg cgtgaaaaga
    35821 aagttcgcgt tgactccatc aaaactaaaa aattaaaccc agttggcgcc gctaagaagg
    35881 cagaagcccc aaagagacct ctcattaact cggtatttaa cctcgccaaa caaggctaag
    35941 catgaacgct ccacaaaaag ttccccttca acctgtttcg ctctatagcc cgaagcattt
    36001 tccggtctac atggattact cggcaaccac gccgattgat cagcgtgtgg tcgataagat
    36061 gcttccctat ttgcgggagc agtttggcaa tgcagcctcg cgcagtcatg cgtttggttg
    36121 ggctgccgaa gaagctgttg aggaagctcg tgtggaagtt gccaagctag tcaatgctga
    36181 tccccgtgaa atcgtgtgga ccagcggtgc gacagagagt atcaatttag cgatcaaagg
    36241 cgctgcccat ttctataaag agaagggtaa gcacattatt acggtgaaga ctgagcacaa
    36301 agcgacgctc gatacctgcc gtgaacttga gcgtgagggc tacgatgtta cgtatctgga
    36361 tgtgatggat aacggcctga ttgactttgc acagctcgaa gctgctattc gcccagatac
    36421 cattttggta tcggtcatgt ttgtcaataa tgaaatcggc gttattcaag atattcccaa
    36481 aattggagaa ctttgccgct cacgtggaat cattttccat gtggattctg cccaagcaac
    36541 cggtaaagtt gatattgatt tagagaagca aaaagttgat ctgatgagct tctcggcaca
    36601 taaaacctat ggacctaagg gtattggcgc gttgtatgtt cgccgcaaac cccgggtgcg
    36661 cattgaagcg caaatccatg gcggtggtca tgagcgcggc atgcgctcag gcaccttacc
    36721 tgtccatcaa attgttggta tgggcgaggc gtttcggata gctcgagtgg agatggttga
    36781 ggagagcgc
//
LOCUS       DDPQ01000093           46229 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2475,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000093 DDPQ01000000
VERSION     DDPQ01000093.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 46229)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 46229)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..46229
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2475"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    843..881
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1601..1644
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13036..13047
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    27749..27794
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    28796..28817
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    33267..33326
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    40817..40873
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    45179..45243
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        12457 a         9858 c        10918 g        12643 t
ORIGIN      
        1 gcaaagtctc aagttacatt gcgctacgtc aatggtaagc ctgattcgat tgataccgta
       61 gttctctcca ctcagcactc cgctgatatt cctttggaga aattacgcga gtccgtgatt
      121 gaagaaatca ttaaaccagt attgccaaag aacttaatca aaggggatat taaatatttg
      181 gtaaatccta ctggtcgctt tgtaatcggc ggtccacagg gtgattgcgg cttaaccggt
      241 cgcaaaatta ttgtagatac ctacggcggt gccgcgccgc atggcggcgg tgctttctcg
      301 ggtaaggatc cttccaaggt ggaccgctcg gcagcatatg caggtcgtta tgttgctaag
      361 aatgtggtag ccgcaggcct tgccagtaag tgcttaatcc agatttccta cgcaattggt
      421 gtggcgcgcc ccacttcggt gatggtgagc acctttggga ctggcaagat ccccgatgag
      481 caaattgcga aattggtatc agagcacttt gatctgcgac ccaagggtat tgttaagatg
      541 ctggacttgt tgcgcccaat ctataaaaag acagccgcct atggccattt tggccgggaa
      601 gagcctgaat ttacttggga gcgcaaggat aaggctggag ccctaaaggc agcagcaggc
      661 atttaacaga aaacttgttt acaatttgag tattcctcca aggagcgttg caaggggccg
      721 tgatttatat gcgcctccca ggcttggagg ggagttactt ggtaacttca ttgcaactgc
      781 gctcgctaac ccgtattgtc aattggttag cgagtttcat gaacttgctc cgcttgacga
      841 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttggctgac tttggtcgta
      901 aagaaattaa gattgctgaa acggaaatgc caggcctgat ggcaattcgt gaagaatttg
      961 cagcaaaaca acccttgcga ggagctcgta ttactggttc attgcacatg accatccaga
     1021 ccgcagttct gatcgagacc ctcgaggcct tgggcgctga agtgcgttgg gcctcgtgca
     1081 atatcttctc aacccaagac catgcggcag ctgcaattgc agcaaatggt acccccgtct
     1141 ttgcgatcaa aggcgagacc ttggagcaat attgggatta cacccacaag atttttgaat
     1201 gggccgatgg tggctttacc aatatgatct tagatgatgg tggcgatgcg accttacttt
     1261 tacatcttgg taccaaggca gaaaaagatc aggcagttct taatcatccc acgagcgagg
     1321 aagaacgtat tttgtttgcg gccattaaga gcaagatagc cgttgatccc acttggtatt
     1381 ccacgcgctt ggcgaaggta aatggcgtga cggaagaaac cacaactggc gtgcaccgac
     1441 tctatcaaat gcatgctaag ggcgaacttc gcttcccagc cattaatgtg aacgattctg
     1501 tgaccaagag taagttcgat aacttatatg gttgccgtga gtctttagtt gatgcgatta
     1561 agcgcgctac ggacgtcatg attgccggca aggttgcggt nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnatggaa gggtatcgcg tcgtaacaat ggactacgca
     1681 gcagataaag ccgatatttt tgtgactgct acgggtaact atcatgtaat cacgcacgat
     1741 cacatgaaga agatgaagga ccaagcaatc gtttgcaaca ttggccattt tgataatgaa
     1801 atcgatattg cgggcattga aaaatatact tgggaagaaa ttaagcccca agtagatcat
     1861 gtgatattcc ccgcagcaaa tggtttaccc gagaagcgcc tgattatttt agcgaaaggc
     1921 cgtttagtga atttgggttg tggcaccggt cacccctctt atgtaatgag ctcatcattt
     1981 gcaaaccaag tcattgcgca aattgagttg tggggagcag ctggtacgaa taaatatcca
     2041 gtgggcgtat ataccctgcc aaagcatttg gatgaaaaag tcgcacgttt gcagctcaag
     2101 aagctaaatg cacagttaac ggagttaaat gatcaacagg cttcttatat cggcgttaag
     2161 aaagagggcc cattcaagcc tgaaacttat cgttattaat gacgctgttt gatcatgaat
     2221 gtagccacta gtccatggaa ttaagcatcg agttcttccc ccctaaaacc ccagaggggg
     2281 aggtgcgtct tgcgcaaacg cgggagcgct ttagcgccgc tttacaacct gcgttttact
     2341 cggtgacctt tggcgcaggc ggatctacgc aggcgggcac tctacaaacc gtgcgaataa
     2401 tccacgagac aggtgaagta gtggctccgc atttgtcatg cgtgggtagc tctaaagcgc
     2461 tgatcgccga tctattaaag cagtatcagg cattaggcat caagcgcatc gttgcattgc
     2521 ggggcgattt accatcgggt atgggccagt atggtgagtt tcagcacgcc aatcagctgg
     2581 ttgagtttat tcgtcaagaa accggagact ggtttcatat tgaggtcgcc gcttacccag
     2641 agacccatcc ccaggctgag tcaccgagta aggacgtgca gttttttgct tcgaaggtga
     2701 aggcaggcgc agattctgca atcacacaat acttctttaa tagcgatgct tacttccgtt
     2761 ttgtcgatga tgcctatcaa ttaggtgtga atgtaccgat tgtggctggc atcatgccga
     2821 ttacgagtag tactcaattg cttcgcttct cagacgcttg cggtgccgag atccctcgtt
     2881 ggattcgtct gcgattgcaa tcgtatggcg atgatctggc atccatcaaa gcttttgggg
     2941 aagaggtagt cacaacactt tgtgagcaat taattgttac gggtgttcca ggcctgcatt
     3001 tttactcgct caaccaatct gagccagttc tgcgcattac ccaaaactta caactgggta
     3061 aaggctgatc caaaatcaat ttgctttatt tgggataaat cattgagttt gtaaccacgt
     3121 agtccagagc ctggtcatgg tcttggggaa cccaacggtc tgcgctaagc ttttgccaat
     3181 cataggcaac cccaatcgtc ttaaaggtat gcccctcatt ttttagggtg gcaatggtgc
     3241 ggtcaaaata gccaccccca tagcctaggc gccataggca atctttttga agactccaac
     3301 ccacgcaggg gatcaaaata cagtccggtt gtgccaactc aattcccgga gactcaggat
     3361 ttggctccag aatcccatat gcattcgtaa tcaaggcgca cgattctgac caggtataaa
     3421 aatccaagcg cttatctgat cgaaccttgg gaagcaggag cgatcgttga ggtgctgatt
     3481 gctgccactg aagaagggga gccttgaggt ctggttcttc tttaaaaggc cagcaaaagg
     3541 cgattgaccg atagttcgag tctggcctct ggagaagctc gcctagctta gccagaagac
     3601 ctaaatgggc tccttcaaaa gctgtacttt gcctgtaatc gactcgattt tgcaataaat
     3661 catggcggac ttgatcaata ggggtatgca tagcttctat tatcgagtga aaatagaact
     3721 atgtgcccta atttatctat ccgtctcact gtacggcgag gctttagctt cttagtctgc
     3781 gctttaacct tgaatctagg cctatggcaa ttcagcattg ccaaagcccc taccgcttcc
     3841 aaatcccaac agaatcagcc cgtatctcag gagttgtccg aagctgataa attgttcatt
     3901 gagctacgcg atgcggcacg aaagaatgat gtcacgcgaa gcggtcaatt ggcagcagcg
     3961 ctggttaatt atccctatac cgactatgta cggtatttca gaatcaagcc acaactattt
     4021 gataaaggta atgaggcgcg cgccgatacg atactcgatg aagaggtgta tgcattttta
     4081 agagcatatg aggggactgc gattgcagat cgactacgta acgattggtt gctcgttctc
     4141 ggtaagcgca aagactggcc acagtttgat aaagagtatt cactcttcat gttggatgac
     4201 gacaccaatg tgaaatgcta tgcattgcag tcacgacttg cgaaacagga agatccaaaa
     4261 cggattggcg aagatgcttt aaaaatcatg atcgatccac gcaattttgg ttcagcatgc
     4321 caagagctag ttcccctttt agtacaagcc aaagggctga caatctctca ggcgaaagcc
     4381 ttggggcgtg cagctgccga aatgaattta gagaccattg gaaaacggat tgggggcgat
     4441 gatctgcttg cgacgcttgt gcgttcagct cgcgctgatg ctagtaaggc ctaccgtgag
     4501 tttttacaat cgaacgctcg gctcagtaaa gaggatcaag caatggcctg gggcgtgctt
     4561 ggacaatttt tagcaaaacg cttagaccga aacgcgattg atgcgtatcg gcgtcaacag
     4621 caactcggct ttgatgctct tttgtcaacc gaaagccaag agtggaaagt gcgcacggcc
     4681 ttacgggctc acgattggca gttaaccaag gatgcgattg agaaaatgaa tccggcagta
     4741 agacaacgcg atccagcatg gacctattgg tatggtaggg cctataaaga gctcggcagt
     4801 cctgaattag ctaatgcaca atttaaggag ttagaggatc aatatcattt ttatgggcag
     4861 ctggcgcgcg aggagcttgg taaagcaatc tttattccgg ccaaagtaaa agttagcgag
     4921 tcagaaatta aaggaatggc agggcgcaat ggctttgttc ggggcgaacg gttctatgca
     4981 atgaatttac gctttgaagg gaatcgtgag tggaattggg agttgcgcgg tatgagcgat
     5041 aagcaattgt tagctgccgc agagtatgcc aaacggatcc acctatacga ccggaccgtt
     5101 aatacggcag atcgcacaaa aggggagcat gactttgcat tgcgttaccc caccccattt
     5161 aaagaacaat tgagtccaat tgccaagaat attgatttaa atctcgcgtg ggcatatggt
     5221 ttgattcggc aagagtcacg atttataaca aacgcctcct cttcggttgg ggcatcggga
     5281 cttatgcaag tgatgcctaa tacagccaag tatgtcgcca aaaaaatagg gatgaactcg
     5341 tatacgaatg agcgcctaaa ggatacccag acaaatttaa ccctaggtag ccactacctc
     5401 aatatggttc tnnnnnnccc tctcgaccaa aatcgtggcg cgagaagtta acgcaatcaa
     5461 cggagggcgc gatttttgtt gagtctattc cgtttaatga gacccgcacc tacgtgaaaa
     5521 atgtcttggc taatgcaacg tattattctc ttttaatgga tggaaagtcg agcacgatca
     5581 agcagcgcct tggtaccatt cagccaaaaa cagccgtatt aagcgagcta ccatgaagca
     5641 cgatgtatta attattggcg gtagtggttt tgttggaaaa accatcgctc agcagttgca
     5701 gcaaaagggt tactcggtat taataccgtc aagacgctat agcgctattc gtgatctccg
     5761 cttattaccc agtgtcactc ttgttgagac cgatgttaat catcgggaag caattcatga
     5821 tctcttggca gagttaaagc caacagccac agttattaat ttagtcggga ttttgcatga
     5881 ccgtacgggt catccttatg gcccaggttt taagaaagcg cacgttgatc ttccagaaat
     5941 gcttatgcag gagatgaaag tttttggcct aaaacgatat ctgcatatga gtgccttagg
     6001 agcgcattct caaggacctt ccatgtatca acgaagcaaa ggagatggcg agcggtgtgt
     6061 cgagcaaagc ggacttgatt ggactatttt tagaccctcg gtcatctttg gtgagcaaga
     6121 tcaatttatc aatacctttg tgggcttggc taaacttttt ccggtgattc ctctggccaa
     6181 tacccaagct cttttccagc ctgtgagcgt caatgatgtt gcgaaagcat tcgtaatggc
     6241 cctagaggat cagcgtacga tccatggtat ttatgatttg gtcggtccgg aagtatttag
     6301 catggaagat ttagtacgat ttgcaatccg taaagcaggc aaaaagaacc cagtgatttc
     6361 attgcccaat tgggtaggat atctgcaagc tattgccttt gagtttgggc ccggaccgac
     6421 gctaatgtcc cgcgataata ttgcttcgat gagcgttcct aatgtattgg cactcggtag
     6481 tcgtgactcg ttaagcgaag actttgggat tgctaaacag catttagaaa gcttaattgc
     6541 ttgaagattt atcaagttgg tggtgctgta cgcgatcgct tgctcggttt accagttcat
     6601 gacattgatt atgtggtggt cggatcgtcg gttgaggaaa tgctagcgaa gggctttcgt
     6661 cccgttggta gtcattttcc agtctttttg cacccacaaa cccagcatga gtatgccctt
     6721 gccagaacag agcgtaaaac cgcacctggg catcaaggct ttgtatttca tgcagatcca
     6781 agcattcggc tcgaggacga tcttaagcga cgcgatttaa cgattaatgc catcgctcaa
     6841 gaagtaaacg cttcgggcga gctcatcggg gatttaattg atccatatca gggcaaagca
     6901 gatttagagc aaggcatatt tcgtcatcta tcgaatgcgt tttcggaaga tcccttaaga
     6961 gtattgcgag tcgcacgatt tgccgcccgt tttccgaagt tcaatattgc cccagagaca
     7021 ctcgttttaa tgaaatcaat ggtgcataac ggtgagctta agaccttatc caaggagcga
     7081 gtttggcaag agcttgcaaa agcgtttata tctcaagcgc ccgaatgctt tatagaagtc
     7141 ttagaacaat gcgatgcgct tgaggaaatt tggcccaatg cgcttgttaa gcagtggcag
     7201 catatgtcaa gcagaaaaga aatgcttgcc cagttacatc aggcagttac cgtatatcca
     7261 gagctagaca aacgcttagc gattgtctta aagcaactaa agcggtcaga gattgttgat
     7321 tgggcaaatg ggttgagagt accaaatgac attacagagt attgcctact tactgctgat
     7381 tttgaagcgt gccgccactc ccccattcat ccgcagttag cccttgattt gcttaatcgc
     7441 tccgatgttt ggcgtaaacc aaaacgcttt ttagaggtac ttcgtttagc aaatacgctc
     7501 ggtattgata ttcaggaatg gcagaattta atggaggctg ctctgcagat tgatgggggg
     7561 cagattgcag cagagatcag tcaacaaggc ataagcacta atcatggcac gcaaatacat
     7621 caggcgcttg cgcatgcacg tctaatggcc attacacgga cacttgatca ataaaatcgg
     7681 ttacggcctg caaagccagg taattggttt agttgaagat tgagtgtctc tagatttttt
     7741 ccaaaagcga tcaccttaaa gagttcgccc atttctgctt cagatattaa tttttgtagc
     7801 gcatttgaaa tcggaagaaa gtgctctata tcgtgcggat ctgatttttg taatacaagt
     7861 tccgtaatgc ctgcatcgag taaataggat gcttggcttg agaaaaaaag ttcgccatgg
     7921 atactttcct ctaatattcg caataggcta gagcactcaa tatggtttgt gatatcggag
     7981 atgccaggaa attgcagcac atcggtcagc gcacgatggc gatgatgagc cattaaggtt
     8041 ccagattggc gctgagggtg gtagtactct tgctcatgaa agccatagtc aatgattagt
     8101 aataagccgc aagccaattg ttctgtaagc gatctgagcc aggcttgtgc ttgtagatgc
     8161 acttctgttg tatagccatc agcaaatgct tcagtattta gtaatactgg aggtatctta
     8221 ttaagttcag cttggcttaa ggcggcacct atggtggaaa cgagtgatgc tggagaagaa
     8281 tctgtatcga ctgcaatatc aagacgatgc catatgttgc gatgcatgca aattcgctcg
     8341 catggaatag catctaacac ctcatttgct aagactaccc cctcaatatg attagggata
     8401 gagcttaacc aaatgcactg cgtatgaatc gcatgctcat gacagatatt ttttaaaaga
     8461 agttgttggc gtttagcaag gtcaggtgag agttcgataa tttgatattc atcaagatca
     8521 aatccacgta aatgcaattc cagcagaatc gattttgcaa gactgccggt gcccgcaccg
     8581 aattccaaaa cacgggtagg aaggccctgt tgcttaagag cctctaagac gggcaagatg
     8641 gtacttacga tggtttgtcc aaaaaaagga ctaatttcag gagcggttat gaaatcacca
     8701 gcgaccccaa atttttctat gtcagaagag taatagccca aattaggctc atacagggca
     8761 atatgcatat acctagaaaa ggccagatgc cccccatttt gcttaatttc ctgggaaatt
     8821 ttagccatca tcgcggccaa atgctcctct tcgggctggg tcaggttaat atccatagct
     8881 cattagtcta agagagttta cgtggaaggt tcaaagacgt cctcgcatcg tactgttttg
     8941 cttaccggag cggctaaacg cttgggtaaa gaaattgctt tgcacttcgc taaggcggga
     9001 tggaatgtgg ttttacatta cggcagctct aagcaagatg ctttagagac ccttgcaaaa
     9061 attaagagct tgggagttga ggcaattgca attcaggcca acttggcaga ggccgaagaa
     9121 atcaaggttt tatttaaagc aagtatcgat caatttaaca gaattgattg cttaattaat
     9181 agcgcctctg tctttgaata cgatcgaccc agtcaggcaa tgcatatgct gacacagccg
     9241 agcctcatgc gtaatatcca agtcaatcta gccgcaccag ttttgctggc acaagaaatg
     9301 tttcatcatc tccaaaagca gcctgctcgt aatgatggtc tcgttccggt aacgattcag
     9361 ttactggatc aaaagctgat taatttaaat ccggattatt tctcctatac attgtcaaag
     9421 agcgcattgt taacagccac tgaaatgatg gcgcttgatt ttgctccaat catgcgctcg
     9481 gtcggtttgg cccccggcat aacactgcca tctggtcaac aaacagaagc agaattctta
     9541 cgcgcacatc gaatgacccc aatggggtat tcatccagtg cagatgatat tgcacaggca
     9601 gcattattta ttgctaatgc aaaagcaatc acaggcacaa ccatttatgt cgatggcggt
     9661 cagcatttgc tgtcatcgga gcgtgatgtg atgtttaaca ctcaataagg tcgctatgca
     9721 ctccctttta tcgcatccca gtctaataaa ttgccgccgc ttatttttaa aagactatga
     9781 ggtttatatc aatattggtg ttcatgattt tgagaagaaa gcagagcaac gcgtaatttt
     9841 taatgttgat ctttatgtgc ctcttgagca aaataccccc atgcatgatc gattggatga
     9901 ggttctggat tatgacttta ttcgtaaaac aattactcaa agagtccaga gaggacacat
     9961 tcatctccaa gaaactctat gcgacgatat tgttggggca gttttagagc accctaatgt
    10021 aatggctgcg agggttagta ccgcaaaacc agatgtttat cccgattgcg aggcggttgg
    10081 ggttgaggtg ttcaagatta aacaggttag tcaataactc tcatcttatt tattgactat
    10141 ggcagatcct cgtaaacaag cttttgaaga gaataaatta gataaaaaat tatctcgttt
    10201 ggtgggcgag gcaattggtg actttgggat gattgccgac ggtgataagg tgatggtatg
    10261 tatatcaggc ggcaaagata gttatacctt actagatatc ttattaaaac tacgcgagcg
    10321 tgcccccatc tcctttgaga tcgtggcagt taatttggat cagaagcagc caaattttcc
    10381 ggcggagata ttgcccaact atttaaagca actaaatgta ccgtttcata ttgaagagca
    10441 agatacgtac agcattgtaa aacgagttat tcctgaggga aaaacaacct gtggcttgtg
    10501 ttcgcgttta cggcgcggta ttttgtatcg ggttgcagat gagctcgggg ctaccaagat
    10561 agccttagga catcatcgtg atgatatttt agaaaccctt ttgttaaata tgttctttgc
    10621 agggaagctg aagggtatgc ccccaaaatt acgctctgat gatgggcggc atattgtgat
    10681 tcgtccgctt gcctatgttc cagaaaagct gattgagcgt tatgctacct ccatgagctt
    10741 ccccatcatt ccgtgtgatc tatgtggtag tcaacccaat cttcaaaggg cagccatgaa
    10801 gaagctcttg cgggactggg agaagcaatt tcctggccga gttgagaata ttttccgctc
    10861 aatgcatcag attgtgccct cgcatttaat ggatcccaac gcatttgatt tccaaggact
    10921 agagagccat aagaacctca gtgccctggg aataaacctt gagggagatt tagcaattga
    10981 tgagggtgaa gatgtgtgtg tcccgttgaa tcaagcccaa ttcccaattt catttgctga
    11041 taagctttaa tttcttagac caaccgcttt ctcattaacc catctcaagc cctatactta
    11101 tggctatgaa tatagtgata ttggcagctg ggcaaggcaa gcgcatgaaa tctgctttgc
    11161 caaaggtatt gcaacctcta gcgggcaaac cccttttaga gcatgtacta gaaaccgcta
    11221 tttctttaac gaaggcagca cagccagtag tagttattgg gcatggcgca gagtatgtca
    11281 atgcattcct aagcgattta gcgaaccgta atccacgttt taaggcaatt aaaacggttt
    11341 tacaaaaaga gcaaaagggt acaggccatg cgctcttaca gaccctaagc aaattaaata
    11401 aagatttacc aactctcgtc ttatatggcg atgtgccatt gaccacaaaa gatactcttc
    11461 aaagcctcat tcgcttagcc gacggagttc gcgcaaaaca gtcggccctc ggtttattaa
    11521 cccagaatat ggaaaaccca aaggggtacg ggcgcattgt gcgcgatcag gatggcgcag
    11581 taagtgcgat cgttgaagaa aaagatgcca gcccaagcat taaagcaatt catgagatta
    11641 atacaggtat catggttttg ccaagcgagt ctttatcgaa gtggcttaag agcttaaaga
    11701 ctaaaaatgc ccaaggtgag tattacttaa cggatgtgat tgcaatggca gtccgcgatg
    11761 gtgttcccat ccgaacctca caagcaaaaa atgagtacga gatcttagga gtcaatagtc
    11821 gtgatcagtt ggctgcgcta gagcgtcaaa tacaaagaca gcgagccgct gcattattag
    11881 aatcaggcgt taccttattc gatcctgagc gtattgatat ccgtggcgaa cttagttgtg
    11941 gtcaggatgt atggatcgac gtgggttgtg tgtttgaagg acgcgttcac ttggcagccg
    12001 gagttcgtat tggcccttat tgcgttctaa aagataccca gattgctgcc aatacgcaag
    12061 tcgaagcatt cacacatatc gatggtgcac aaattggtgc tgaagcccga gtgggcccct
    12121 atgcacgcat tcgaccgggt acgatattgg cgaacgaagt tcatattggt aactttgttg
    12181 aagttaaaaa cacgaagatt tcggcacaga gcaaagccaa ccatttggct tatgtgggcg
    12241 attctattgt gggggctcgt gtcaatatcg gcgccggcac tattacctgt aactacgatg
    12301 gtgtcaataa gcatcaaacg attattgagg atgatgtttt tattggatct gatactcagt
    12361 tagtggcacc tgtgaaagtt ggtaaggggg ccaccttggg cgcaggaaca accctgacca
    12421 aagatgctcc tgctggaaag ttaactgtgt cgcgcgcgaa gcaagtctcg ttagactggc
    12481 aaagaccgat aaagatcaag cccaaatcta gcaaagtaaa gaaataatct atgtgcggaa
    12541 ttgtgggcgc agtctccaag agtaatgtgg tcgatacctt gatcgagggt ttgcggcgtc
    12601 tggaatatcg tggctatgac tcttgcggtt ttgcagtgat agatgccacc aagacaaatc
    12661 atccactgca gagagctaga acaactgcgc gagtcgctga tttagcgaag caggccaccg
    12721 agtttaatgg ccatattggg attgcgcata cccgttgggc cacccatggt aagccagaca
    12781 cacaaaacgc acacccccat atctcgaatg acttaattgc gctggtgcat aacggcatta
    12841 ttgagaacta tgaggcatta cgtgaacaac tgattgccgc tggatatcaa tttagttctg
    12901 agactgatac cgaggtaatt gcgcacttag ttcatcgcga gtatgtcaaa ttagctaagc
    12961 ctgacttaag agaggctgta gcagcgagta ttttgcagtt aaagggggct tatgccatcg
    13021 cagtcatttc tcaaannnnn nnnnnnngcg atgatggtca ttacgtatct tccgacgccc
    13081 tggcattagc aggaaaagca caaactatga tttaccttga agaaggggac ttggtggcaa
    13141 tcagtgaggg cgatttcaat gtttgggatc gtggcggaaa aattgtaagt cgtgaaatca
    13201 agccaatgcc agtgcaggga gaggctgttg aattgggacc ctatcagcat ttcatgcaaa
    13261 aggagatttt tgaacaggcc cgcgcggctt ctgacacgat tgctcatatt tctgagtttg
    13321 gaccagccct gtttgctgct gatcctaaag aatggcaagc gtttgatcag attttgcttt
    13381 tagcctgtgg taccagttat tactctgcct gcgttgctaa atattggctt gaagaaattg
    13441 cacgcattcc aacccaagtg gagattgcaa gcgagtatcg ctatcgtcag tcaattccca
    13501 ggtcaaaaac tttagtagtc gttgtctccc aatcgggaga aaccgctgat acattagcag
    13561 ccttgcgaca tgcaaaggag cttggtcacg gcatgacctt atcgatttgt aatgttgctt
    13621 ctagtgctat ggtgcgtgag acacaatggc actttttaac gcatgccggt acagaggtgg
    13681 gtgtagcatc tacaaaagcc ttcacgaccc agttacttgc tttgtactta ttggcctgct
    13741 cgattgcaaa gcgcaaagga ttggtatcga gcgatgccga gaaaaaaatg cttcatgatc
    13801 tgcggcattt accacaggcg attcatgcag tattagcgct agagccacaa attatttctt
    13861 ggagtcaatt gttcacaaaa tgcgagaacg cattattttt aggacgaggt atccattttc
    13921 ccattgcttt agagggggcc ttaaagctga aagaaatttc ctatattcat gcagaggcct
    13981 atcctgctgg cgaacttaag cacgggccac tagccttggt aactgaaaaa atgccggtgg
    14041 ttaccgtagc gcccaacgat acgcttctcg aaaagctcaa gtcaaatatg caagaagtca
    14101 aggctcgcgg aggacagcta tttgtttttg cagaccaagg cactcacatt aagagtgagg
    14161 aaggcatacg ggtgattcgc ctgcctgagc actatggaga tttatctccc atcttgcacg
    14221 ttattccact ccaactcttg gcctatcaca ccgcctgtgc tttggagact gacgttgata
    14281 aaccacgtaa tttagccaaa agcgtcacgg tcgaataaat ccgcattaag tatcatcctt
    14341 gtggtcaaat attggatgtg aaaagtcgtt tagttacccc acatttttct tggaaattct
    14401 gtctgtcata tcccagtaaa aatgggctgg cactgattgt ttctcttggt cttggtgcat
    14461 gcgcagtcgg acccgatttt aagaaaccag acgcacctaa ggttagttcg tttacagaaa
    14521 agccggttag caataagtta gcaacagcac caggtgtggg cggaacacaa cagagcatag
    14581 aacctgcagc agatatccca gcagaatggt gggccttgta tcgctctccc gagcttgata
    14641 atttgattaa gcaagcttta aaacgtaatc cgaatttagg tgctgcagac gcaactctga
    14701 gagcagctca agaaaatgca aatgcagctt ttggatcttt tctatttccg agtatcggtt
    14761 taggaggaag tgcaacacgc cagcaaataa atccatccac atttggacag ggctctggaa
    14821 gtcccagtat ttataacatt tacaacacct cggtttctgt gaactataac cttgatgtat
    14881 ttggtggcgc acgtcgtgca gttcaaagtg cagaagcgca ggcagagatt tctggatttc
    14941 agctcgaggg agcctattta aatctgacag caaatatagt gactacggcg attcgtgaag
    15001 ctgcattacg cgcccagtat gaatccaatc tagagatcta cgaagcacag aaaaatctgg
    15061 cggatattat tgcaaaacaa ctcgagatcg gaactgcatc gcgggttgat ctcacttcac
    15121 aagtttctct agcagctagt tcacaaattg atttgctcaa tctgaataaa aacttgtctt
    15181 ttactcgtaa tcagttatcg gtactggtag gtgagtttcc gggtaacggc ttgctggcta
    15241 aatttgattt aaataaatta aatctacccg agaaaattcc cctatccgta ccatcggatt
    15301 tggtccgcca acgccctgat ataagggctg cagaagccta tattaaatct actaatgcct
    15361 tagttggtgt cgcaactgct aatttgttgc cacaaattac cctcagtggc gccacaggtt
    15421 cacaggcatt aacgactggc gctctatttg ggcccaatgc cgcaatttgg tcagttgccg
    15481 gtggagtctt tcagcctttg tttcaagggg gcgcattact tgctcagcgt agaggggcaa
    15541 ttgccactaa cgaagctgcc gtctttcagt atcaggcaac cgtcctgagc gcctttcagg
    15601 aagtggccaa tgcattacaa gcattagaaa gcgatgccct tgccctaaga gcggcatccg
    15661 aagctgagcg gaatgcctta gagtatctaa atttactaga gcaacaatat aaattgggta
    15721 caggaagtta tttggctgtg ttaatcgctc aaaggcaata tcagcaaacc aagtttcgtt
    15781 tgattgatgc acaagccaat cgatttgcga acaccgcagc tttatttgtg gccctcggtg
    15841 gaggttggtg gaatcgcact ggccctgctt atcaggcgca gaagaaagaa gacctccata
    15901 atcaaggatc tagtcagcaa gagatgcgcc aatgaatgca atacacacca tcatcgcttg
    15961 gataaaaaat aaagtcttga gtaaacagaa tctcgacttt gttttgctac agcttaaaaa
    16021 gatagctaac gcaactcggc cacgagagcg gtttagaaat acaaagacat acgctgccct
    16081 gatgcagatg ccaacattaa agcgccgcat gattgtgatg cttgtttcgg tctttgtgct
    16141 ccttggcttg gtcgtgggat ttaatcaatt aaaaaccttg atgttcaatt actttatgtc
    16201 tggtttgggg ttacccccag ctaccgtcac aacgatggtt attgaaaagc aggaatggca
    16261 gcccaagatg actagtgttg gaaacgtccg cgcttttcgg ggtgttgatt taagcactga
    16321 aattggcggc ctggtattaa atgttccaat caagtcaggc ttagatgtta aggagggcga
    16381 tcttcttatt aagctgaatg acgcagcgga tcttgcacaa ctagaggcga tgaaggcaat
    16441 ggctgatctt gccaaggtca ttaatgagcg cgataaggct cagttagcga ttcaggcaat
    16501 tagtaaaaat gtctatgaca ccagcacggc agatatgaaa gccaagcgtg ctcaggtaga
    16561 tcagcaaact gcattggtag ccaaaaagaa tttaaaggcc ccatttagcg gtcgtgttgg
    16621 tattgtgacg attaatccag gtcaatatgt aaatccaggg gataaactac tgaccctgca
    16681 aactattgac ccaatctacg ttgactttac tttaccgcaa agctcagcag gaactataaa
    16741 agctggacaa acaatcgact tacaaacgga tgcatttaaa gattctatat tttctggcaa
    16801 gatcactgcg gttagcccta aagttgatct caatacacgc aatattcaga ttgaagcgca
    16861 gattagcaat ccagataaaa agttattacc tgggatgttt gctaatgtaa acattaattt
    16921 aggcgataag cttgagcttc taaccttgcc acaaaccgct gtgacctaca acccatacgg
    16981 aagtacagtt tttattgctg ttaaaactaa tcgtaaggat aaaaaaggca atcccattat
    17041 ggaggcgcag caagtatttg tgacaccagg gctcaagcgt ggtgatcaag tcgcaattat
    17101 gaaaggccta gaaccaggca tgactgtgat cacgagcggc caattaaagc taaaaaatgg
    17161 taccccactc attgtaaata ataacgtctt gccggctaac tcgcccaatc ctaaaccaca
    17221 agagcaatga gtcaggactt tacgcgtcat caatggacgg atatttttat tcgtcggcca
    17281 gtgttgtcaa tcgtagttag tctcttaatc ttattgttgg gcttaaaagc tgttggatcc
    17341 ttacccgtta atcagtaccc ccaaacccaa aatgcagttg taacgattac gacagcttat
    17401 tttggtgctg acccagaaac catcgctggc tttattactc agccattaga aggcgccatt
    17461 gcccaagctc agggcatcga ttatttgtca tcaatcagta ttggcggggt atcaaccatt
    17521 tctgctacct tgcgcttgaa ctatgacgca aacaaagcgt tgacacaaat taatacgcaa
    17581 attagttcag ttcgcaatca actaccaccg caagctcagc agccgatctt aacggtgcaa
    17641 attggccaat caacagccgc tatgtatatg ggcttttata gtgatgagat tgccaacaat
    17701 agcattaccg attatttatt gcgggtcgtc aagccaaagc tcgactctat tagcggtgta
    17761 caaaatgcgg agatcctagg ggggcgtaaa tttgctttac gggcatggct agatcgcgat
    17821 cggatggcgg gattaggggt ttctccagac gaggtatatg cagcacttgc tgctaataat
    17881 tatttatcac ctgtaggcag caccaagggg caaatggtat cggttgactt ggtcgctagc
    17941 actgacttgc attcgctcga tgaatttcgg aaattggtca ttaagcgaaa tggtgctgat
    18001 attgtttatc ttgagcaagt tgcaaccgta actctaggct cagaagatta caacaataat
    18061 gttgccttta atggcaaaca atctgtgttt attggcatta aggtagcgcc tgatgctaat
    18121 ttgcttgatg tagcaaagcg cattcgaaac acattccctg atattcaaag gcaacttcct
    18181 tctggtttaa atgccaaaat cgtatttgac tctactgact ttattaatac atcgatcgaa
    18241 gaggttgtca aaactcttct agaggcatta attattgtga cccttgtgat ttatttattt
    18301 ttgggtagct atcgagcggt agcagtcccg gtgctagcaa tgccactctc tttgattggc
    18361 acttttttta tgatgcaggc acttggctac tcaattaatt tactcacctt gcttgcctta
    18421 gtgctagcta ttggattagt ggtagatgat gcaattattg tggttgagaa cgttgatcgc
    18481 cacatgaagg agggtaaatc acctctcgag gcttctctaa ttgcagcccg agaattaggt
    18541 aatcctattt tagcgatgac ggttgtgttg attgcagttt atatccccat cggttttcag
    18601 ggaggcttaa ctggcgcact cttcactgaa tttgctttta ccttggcagg agcagtggca
    18661 gtatctggcg tagtagcgtt gaccctttcg cccatgatgt gttcacaaat cttctcgcag
    18721 gaagaagaaa tgtcgccttt cgtgaaaaag attgatgcgg tattcgagcg agtgcgccac
    18781 tcatatcaag aaacacttcg tgacctttta tcaacctggc aggtcattat tgtgatgggc
    18841 gctttgcttt tagggggcgt tgtttatctc tatgcaaccg cccgttcaga gttagctcca
    18901 acggaagacc aagggattgt tttgatgtca gcgattggac caccaaattc aacggtgaac
    18961 cagatgcaag gctatgctga tcaaatattt cagattgcaa gtaatgaacc tgagtataag
    19021 cagatgttcc agattacgag cccaacatct agttttggtg gcgtgatcat gaaagactgg
    19081 ggtgagcgca gccgcagcgc gaccaagttt caagaagact tgcaaaataa gtggagcagt
    19141 attgctggaa cacgggtggc agcgtttcag ttcccagcat taccgggtgc gcagggattt
    19201 ccggtccagg tagtgattaa taccaccgag ccctatacca atttaaatga ggtatcacag
    19261 caggtcttag ataaggcaag gcaaagtggc atgttcttct ttgtggactc tgatttgaag
    19321 attgataagc cacaagatgt cttacaaatt gatcgcgaga aagtagctgc cttagggatg
    19381 acccaaagag atgtcggtaa tgcgctatcc gctgcattgg gcggtgggtt tgtgaattac
    19441 ttttcggtag caggtcgttc gtatcgggtg atcccgcagg ttaaacaaac cgatcgatta
    19501 aatcccgatc aaatattaga ttactacatt aagacaccaa gtggagcgat ggttcaagca
    19561 agaacaattg caagtatcga aagccgagtt gtgccacagt caatcaatcg gtttcagcaa
    19621 ctcaactctg cgactattag cggtgttagt acgccatttg tgtcgcaaga ggaagtatta
    19681 agtttcctag aaaaaacgat taaagaagca gccccttcag gttactcggt tgactacgct
    19741 gggccttcgc gacagttcaa ggcagaatct ggcggctttt tggtcactat ggtctttgcc
    19801 gtgttaattg tgtttttagt tttagctgcg cagttcgaga gcttcagaga ccccttggtt
    19861 attttggttt ctgtaccact tgccttattt ggtgcgctga tctttattaa tttaggcttt
    19921 acgaccttaa acgtatatac ccaagtaggc ctagtaaccc tgatgggctt aattagtaag
    19981 cacggtattt tgatagttga gttcgcaaac gaattgcaag aggctggacg tagcaagctt
    20041 gaggcaatta ttgaggcaag tagcgtacgc ttgcgcccta ttttgatgac caccgctgcc
    20101 atggtgcttg gcgtggttcc cttggtgatt gcatcagggg ctggcgcagc cggacgtcaa
    20161 tcgatgggga tagtgatttt tactgggctt gcgattggta ctctatttac tttatttgtt
    20221 gtgccagcca tgtatctgta tatcggcgcc gatcatcagc aaaagaaatt taagcaagct
    20281 taatcgctat tgaatcgctt ttgtaaaatg agtgttcgta caaatcattt cgtaccagcg
    20341 tttgatgtgt atcagctctg tccgtagacc tgttttatca gggtactgat ctgccagcat
    20401 gatccagcgc ttaacgcata gtgcaatagt aatatcgccc agattaaatt tggagcccga
    20461 gcaaaaagct tgattggcaa ggacgccatc aaaaataccc aaaagtcgat tagattcttg
    20521 gaaagcattt ttgatggcag cgtaatcacg ttcttcctcg gggacacgag taaggccaag
    20581 aaatggaacg cgcaatgatg gccatagagt actaatttgc caatccatcc atttctcaga
    20641 agaggcttgg cttgcaagat catctgggaa ggcattattg gagtcatact ttttagcaag
    20701 atagcgcatg atggtattgg attcccacag aacaagagct ccatcttcta gggttgggac
    20761 caggccattg ggatttagtt ttaaataatc cggggtctta ttgataccaa aatgtagacc
    20821 cgcatcaatt tgtttaaagt cagatccctc gtccaagcca agttccgcga gtacccaaag
    20881 tactttttgg acattaatcg acgattttct accccaaagt gtcatcattt gatactacct
    20941 tctggatcaa gagcaagttg attaaattgg ctttgtgcaa acaaaagtgg atggcgatct
    21001 gaatcttgct cgatactcaa aacacgcgcc acgataatat gatgatcgcc gccttcatga
    21061 acagcaacag tctcgcattc aaaataagcc aaacaattag caagacgcat taaaccgctt
    21121 ggctcaatcc ggtgttccat ttgaatacta tttttattaa gttgttttgc aaagacaaga
    21181 gccaactctt tctgattaat atctaggaca tgaattagtt gcttctgccc aatacggaaa
    21241 caattgcata agtccgattg actgccaatg ctccaaagta ccaatggggg gttgagcgat
    21301 accgtgttaa atgagcttat tgtgattcca actggttgtg catcttcatt taagccggta
    21361 atgaccgtga caccggtggc aaagcaagaa aaagctcgtc tgagttcctg ggcagaaaat
    21421 tgattttgca atgacttcta cttttattta gagtttgagg gttttaaacc aggaataggg
    21481 ataagcagtt cattttcttc ggctttcaca cacttaaagc gatagtcttt gccagctttt
    21541 gatatgaaac tcgctccaat ataaaaatca tccttacaaa ttttattagc aaaatccatg
    21601 acttgttggg gctcagcact agaattaatc aggcgcatat tgcccaaaga catcttcagc
    21661 tcagaagagg cgcatgcgct tagaaaaaga ggtgctgcta gcaggatgaa tacaatacgt
    21721 ttcatgtaaa agcctcataa tgaactttga agtagtagga taaacgcttt tgcaaaattt
    21781 gatttagtta tcccattcat ttactgattt aggaaaaaaa tgtttagagc cattttaata
    21841 aataaagatg agcagggtta ccgtgctcaa ttgagtaatt tagatgaaaa cgctttgcca
    21901 gaaggagatg ttcgcatcaa ggtgcattac tccactctaa attacaaaga cggtttggcc
    21961 attaccggta agagtccgat tgtgcggagc tttcctatga ttccaggaat tgatcttaca
    22021 ggagaagtga ttgagagcag cagctcggag tttaagccgg gcgatctagt tttattaaat
    22081 ggttggggag tgggcgaatc tcattggggt ggattagctc agcaggcgcg acttaaatct
    22141 gaatggctaa taccccttcc taaagcattg acagcaaagc aatcattggc cattgggacc
    22201 gctgggtata ccgctatgtt gtgcattatg gcgctccaaa agcatggtgt gcgagtgggc
    22261 gatggtgaga tattagtaac cggtgcagcc ggaggagtag gcagttttgc aattagctta
    22321 ttgcatgagt tggggtatac cgtggtggcg agtagcggtc gtccagaaga gactgattat
    22381 ttaaagtctt tgggcgccag tgaggtaatt gagcgcgcca gcctttctca gcctggcaaa
    22441 cccttggcaa aagagcgctg ggctgccgtg gtcgactcag tgggaagcca taccttggct
    22501 aatgcttgtg ccgcaaccaa agcgaatggc gcagtagcag catgtggtct ggctcaaggg
    22561 atggatttcc cggcaagcgt tgccccattt atattgcgcg gtatcaccct gtatggcatt
    22621 aacagcgtca cacaagtaaa ggcagtgcgg atagaagcct gggaccattt agcaaaactt
    22681 tgcaaaccgc aacaactact ggagattgct aaagagatct ccttagaaga agccatccag
    22741 gctagcagtg atttgattga cggtaagatc cgtggccgcg taattgtgga tgtaaatcgt
    22801 taaatacacc tatgtcacta gtgagcccac tgatcacaaa gtttaagaaa atttttgggg
    22861 aaagatattt tttagacgaa gagagtgatc gtactcaata aaatctggaa tctctccaga
    22921 aagtaagcga tctttatagc tttgccacat tgctggatca aataaatcag catgatgctt
    22981 cagcagtagt tgtttaatac gatcatttcc tagtaaaaag gttttataag tttcagggaa
    23041 gatatcttta ggcccaatgc gataccaagg ctcacttgcc atttcatcgt cttcattgcg
    23101 tggtgccggg acgtgacgta cctcacaatc agttaggtac tcaatttcat cgtaatcata
    23161 aaaagcaaca cgaccatagc gcgttacacc aaaattttta tagagtaagt ccccagggaa
    23221 aatatttgca gcaattaagt tcttaatcgc ttctccgtac tgaattaccc cgtcctctac
    23281 ttcgcttgga gagccctctt gcagacggat attgagcggg gtcatccgtc tttcaatata
    23341 aaggtgcttg atgatgagct ctttttctcc agtgggattg atactaagtt ctaataagga
    23401 ggagcactgc tcttgtattt cagaaagtag ctcaggcgtg aagcgctcta aagggaatgc
    23461 tacattagaa aactctaatg agtcggccat gcgccctaca cggtcatgct gtttcactaa
    23521 ttgatatttc gccataattt gttcacggct cgtttccttg ggcggtggga aataatcttt
    23581 aattagctta aatacatagg gatagctagg taagtcaaat accagcataa ctaatccccg
    23641 aattccaggg gcgatccgaa acctatcggt agagtgcatc aagtgatgtt gaaagtctct
    23701 ataaaacaag ttcttaccat gtttttgtaa gcccaccata ttgtaaattt ctgcacgtgg
    23761 cttgtgaggt aagaggctac gcataaatgt gacataagct gatggcacct ccatatcaac
    23821 taaaaagtag ctgtgtgtaa aactaaatat cagacgcaaa tgtacttcag taagtaagac
    23881 cccatcaata acaagttggc ctttcgggtt atgcatcaaa gctataacaa gagggaatat
    23941 tttctcccca ttaataatgc gacccataat ataagccgtt ttatttctaa aaaataaagt
    24001 actgagagca tgaatttgaa aatcggtcga gctattggta tcagctaatg agttttcaat
    24061 taactcagtc gtgagagtga gatcacgctc tagattctca aagggatttt gtaactcaaa
    24121 actcgtcagt aagttgcgcg tgactgtcct aagtccacta atatcaccag gatagtaggc
    24181 tcgatacgat ggtttacttg gcgcttcatc attctcaaga tattcagtgg agatggtagg
    24241 gcgtatgaag ataaacttat tattaaaata ccggctcgct aagatgcgag tggttaccga
    24301 attaaaaaag gtctcagcaa gctctggctg atgtaagtta gacagtagtc caatgtaatg
    24361 aagcttaact tgttgccaga tctcatctga aattgcatgg gcatcaaact cctcttcaag
    24421 cgcttgggcc gtctcaatga cgcgctgatc ataaaaggtg attcggtcgc gtaatagttg
    24481 acggatgttg tgccaatctt gtttctcaaa ggattccttt gcggacttac cagcttccct
    24541 aaaaatactg taatgccgct gaaaacccat taacagggtc tgtgccatat caaaggccac
    24601 ctgcgaggat aatagttttg gaaagggatt gttcataata agagtgtatc tagcatttaa
    24661 actggactca aatgacccaa actaacccac cctctcagcc tgcaagtccg ctgatttatc
    24721 catatgggga gaccctagtg gaagcaggcc acaccatgga gattgcccca gggatctatt
    24781 ggatccggat gcctttaccc tttgctcttg atcacatcaa cttatggttg ttaaaagacc
    24841 gaattgccga tcaagatggc tggacagtga ttgattgtgg gattaccaat gatgagacta
    24901 aaactcattg ggagtctatt tttgcaaaac atctacacgg attgccgatt ttgcgtgtct
    24961 tggttactca tatgcatcca gaccacattg gccttgcaca ttttttatgt gagcgctgga
    25021 aggcgccgct atgggtttcc atgagtgact ttctcatggc acagtggtta agtagtaagg
    25081 agggcggagt tgctgtcggc gccagtagcg gcagtggtgg tgcagcagat cattacgctc
    25141 gtcatggttt agtaggtacc gaagacctag agcgcattcg aagccgttct gattactaca
    25201 gtcggctagt gccacaggtg ccgcgtcgtt atcgccgcat catggagggc gatgaggtcc
    25261 aaattggaga tcatcgatgg agggtatcca tgggctatgg tcatgctcct gagcacgtca
    25321 ctctttattg cacaaaactc aatattttta tttctgggga catggtgttg cctcggatct
    25381 caacgaatgt cagtgtgtat gatgccgatc ctgatgctga ccccttgggt ttatatcttc
    25441 gctcattaga gcagtggcta tctttaccgc tagacacctt cgtgctgcca tcacatggaa
    25501 agccctttag gggcctacat actcggattg agcagttgca tgagcatcat cgcgcccgtt
    25561 tatccgaaac ccttgaggct tgccaaaccc ctaaacatgc ctttgagatt attcctgttc
    25621 tatttcgacg aaagcttgat aatcaccaac ttagttttgc gatgggagaa gctattgccc
    25681 acctgaatta tctgtggcat cagaagaagc tgcggcgctc gctttgcgag gacgggattt
    25741 ggcgcttttg cgcggcttcg cctttgtaga gcttgtctta gcctcagagc tagagcttgg
    25801 cgggtttgcc atggcatccc caaaggattt gattgctaag agggtcgctc tttgaacctc
    25861 caaggtttgt atggtggatt tcagaatatt caggttgagg gaaagccaag actccacact
    25921 tttgaggtct ttaatgcgct tatccagttc ttcaatatca aaagtaggca taccaccatt
    25981 aaagctagac atgcccgagc tcatgccggg cataaaaggg gagcctgcat tcgccgtatt
    26041 attttgatcc cacatggttt tgaacatctc taggctttga ttgaaatccg ggatagcgct
    26101 aaacataaat tctcctaaca ggctaaatag tcacattatc gataaaataa cgggttgatg
    26161 aagattaata ccacttctgc agtaatgcaa acccaaacta agcaatccaa gtcggggaag
    26221 ccctataccc gaggggcagc cttgccttcg ctcctaaggc agcgcatttt gattctggat
    26281 ggcgcgatgg gcaccatgat tcagcagaaa aagtttagcg aagcggatta tcgaggctta
    26341 aaacatagcc agcgctttgc caatcatcca atcgatttaa aaggaaataa tgagctcttg
    26401 gtcttaacgc agcccgaagc cattcgacag atccacgatg agtatctagc agctggtgca
    26461 gacattattg agaccaacac ctttggcgct acctcgatag cgcaggaaga ctacaaaatg
    26521 ccggagcttg ctcgtgagat gaatcttgca gcggctaagt tagctcgtgc agcatgcgat
    26581 caatttagca ccccggataa acctcgtttt gtagccggtg cgattggacc aacacctaag
    26641 acagcaagta tctctccgga tgtgaatgat ccaggcgccc gcaatgtgca ttttgatgct
    26701 ttacgtaaag cctatcgcga acaggcagaa gcattatttg aaggcggctc agaccttttt
    26761 ttagtagaaa caatttttga taccctcaat gcaaaagcag ccctttttgc actcgatgag
    26821 ttttttgagg actcgggaga gcgccttcca gtgatgattt ctggaaccgt tacagatgct
    26881 tcgggcagaa ttctttccgg acaaacagtt gaagcatttt ggaatagctt gcgacatatc
    26941 aagccgctca cttttggttt gaactgcgca cttggagccg ctttaatgcg accctacatt
    27001 gcggagctag cacgtgtttg tgatgttgct atttcatgtt atcccaatgc aggtttaccc
    27061 aatccgatga gtgataccgg ttttgatgag accccagagg taacctcgac tctagtcgat
    27121 gaatttgccc gcgatcactt ggttaatcta gttggtggtt gctgcggcac taccccggag
    27181 catattcgag cgattgcaca agcaatgaca aagcgtcagc ctcgcccctt ctattcggag
    27241 gcgacagcgg aaatatgagt caattacccg ttattccaat gcgcttatcc ggccttgagc
    27301 cctgcaacat ctctccccaa attggctttg tcaatatcgg cgagcgcact aatgtcacgg
    27361 gatcaaaagc tttctcgcgc atgattctga ataatcagtt tgatgaagca ttagccgttg
    27421 ctagacagca ggtcgagagt ggtgcacaga tgattgatat caatatggat gaagccatgt
    27481 tggattcaga ggcggcgatg gtgcgctttc tgaacttgat tgcatctgaa cctgacattg
    27541 cacgcgtccc catcatggtt gattcttcga agtggacggt gattgaagcg gggctcaagt
    27601 gtatccaagg caaacccatt gttaactcta tctctttaaa agagggtgag gagcaattta
    27661 agcaccaggc taaattaatt aagcgctatg gtgccgcgac tgttgtcatg gcttttgatg
    27721 aaaaaggtca agccgatacg tatcagcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27781 nnnnnnnnnn nnnngatatt atttttgatc cgaatatttt tgcaatcgct acgggtattg
    27841 aagagcacga taattatgca gtcgacttca ttaatgcaac ccgctggatt aaagaaaatt
    27901 taccaggcgc aaagattagt ggtggcgtct cgaatgtgag cttctcattt agaggaaatg
    27961 atcgcgtacg tgaagcaatc catacggtgt ttttgtatca cgccatttca gctggcatgg
    28021 atatgggcat cgttaatgca ggtcaattgg gagtctatgc cgatttagat cctgagttgc
    28081 gcgaacgcgt ggaagacgtg gtgctgaatc gctttaaaga aaaagatggg caaagcccca
    28141 ccgagcgttt gttggctata gccgatcaat ataaaggcgg cggcattaag caggaagaga
    28201 atttagtgtg gcgtgaagag cctgttcggg agcgtttaac ccatgcccta gtacatggtg
    28261 taaccaattt tatcgtggat gataccgagg aattgcgcgc tgagattatg ggtagagggg
    28321 ggcggccaat cgaggtgatt gaaggcccgc tgatggatgg catgaatgta gttggcgacc
    28381 tttttggcgc tggaaaaatg tttttgccac aggttgttaa aagtgcgcgg gttatgaagc
    28441 aagcagttgc ccatttaatt ccctacattg aggaagaaaa gaagcaattg gttgccgctg
    28501 gtggcgagag taaagctaaa ggcaagattg tgattgcgac cgtaaaaggc gatgtgcatg
    28561 atattggtaa gaacattgtt actgtcgttt tgcaatgcaa taattttgaa gtagtaaata
    28621 tgggtgtgat ggttccatgt gatcagatat tgaaaaaagc aaaagatgaa aaggctgata
    28681 ttgtcggcct ctctggtttg attacaccct ctttagaaga aatgacctat gttgctcaag
    28741 aaatgcaacg cgatgattat ttccgacaga aaaaaattcc cttattaatt ggtggnnnnn
    28801 nnnnnnnnnn nnnnnnncca gttgtttatg taccagatgc ctctcgctct gtatcggtgg
    28861 cttcgaattt gttatcggat gagggcgcaa agaaatttat tgatgagatg cttgctgatt
    28921 acgagcggat tcggcatcag catgccaatc gtaaaagtgt tccactagtt agcctacaag
    28981 ctgctagaga taattatgag caattggact ggagtaccta taagcccagc aagcctaaat
    29041 ttattggtag gcgtgtattt aaaaactttt ctttggcaga aattgcgaag tttattgatt
    29101 ggactccttt ctttcagact tgggatttgg ctggcaagtt tccggcaatt ctagatgacg
    29161 aaattgttgg tgatgaagcc cgtaaagttt atgccgatgc tcaagcaatg ttagagaaat
    29221 taattaaagg gcagtggttg caagcagatg caattgttgg cttattccct gcgaatagtc
    29281 agggtgacga tatcttgcta tatcccgatg agagtcgcag taaaccattt ttgatttggc
    29341 acaatctgcg tcaacagtca gagcgacctg tagtggatgg agtaaagcgc cctaaccggt
    29401 gtttggcgga ttacgtagct aatcaagcaa gtggtattga agattatgtt ggctgctttg
    29461 ccctgactac cggccacgga gtcgaagaaa aggtggcgca gttcttggcg aagcacgatg
    29521 attacagtgc gatcatgctc aaagcattag cagatcgttt agcagaggct tttgcagagt
    29581 taatgcattg gcgtgttcga acagatttat ggggctacgc tcctggcgag cagttgacta
    29641 ccgaagaact cattgatgag aaatatcaag gaattcgtcc agcacctggt tacccagcct
    29701 gcccagacca tcaagttaag tttgccatgt ttcatgcaat gaaagctgat gagattggga
    29761 tgaccctcac cgaatctcta gcaatgaatc ctgcgtcaag cgtgagtggc ttttatattg
    29821 ccaatcctaa tgccatgtat ttcaatgtgg gcaaagttgc ctccgatcag gtaggtgact
    29881 ttgcgaggcg agcagggcag tcccctgaag aggtaagacg ctctttagca accctcctcg
    29941 actaattaac tccccaaaca accggttcat tattcttttg agcttgtctg agaagttcta
    30001 taaagggaaa cgcacgtcga cccagggggt ccttttttag gccttcagac ttttcagatg
    30061 gctcagtatt ttttggctga gcagttcgtt ctaattgaat ggcttggtcg agtcgctcaa
    30121 tgaaagtagg aatttgctct ggcaacagaa ttcctttttc ttcataaggg cgttgcatca
    30181 ctgcaaagat ccgttcagtg agatcatcta gcatgatgac cggagcgcaa ttcttagatc
    30241 gaaattgatg aatcatagcc tcagcatacc atcactgata aactctcatc tctatgttgt
    30301 tagttcaaaa aaaccaactc gtacacctac ttgccaatgc attaaatgcc attgcgcaag
    30361 agcgcggcct tgccgccgga gaatttccga tccctcactt ggagagacca aaagcagtag
    30421 atcatggtga tgtggcatgt aatatcgcat tacagattgc caaagcatgg aaagcaaatc
    30481 cacgtgaatt agcgcagctg attgtggctc gtttggagtc aagtccagaa tatcaaaact
    30541 taatctccag ttctgacatc gctggccctg gcttcattaa ctttcgctta agccaacatg
    30601 caaagtcggc agttgtacaa atgattctga atgatcgtaa tcgatttggg cggcgcgatc
    30661 gctctggggt gcaagatgca tcgatgccga ctcgtgccat gattgagttt gtctcagcta
    30721 acccaacggg acccttgcac gtcgggcatg gacgtcaggc tgctttaggt gatgctttag
    30781 ctaatctaat ggatagccaa gaaattaaag tgcatcggga gttttattac aacgatgctg
    30841 gggtgcagat tcataatctt gcaatttcgg tacagtctcg cctaaagggc ttaagtcccg
    30901 tcagttcgga ttggcctaaa gatgcgtata acggtgagta tattgccgag attgcacagt
    30961 cgtatcaatc agagcaaaaa gacgagaatg atctagaggc tatccgaaaa tttgcggtgg
    31021 cctatttacg taaagagcaa gacattgact taaagacttt tgggattcgt tttgatgagt
    31081 tttatcttga atcctctttg tattccgatg gaagtgtgca gaaggtgatt gaggatctgg
    31141 caagtgttga caaaacctat gaggctgatg gagcgctttg gctgaagacc actttagacg
    31201 gtgatgacaa agaccgtgtc atgcgcaaat cagatggtag ctatacctat tttgtacccg
    31261 atgttgctta tcacattaat aaatggagtc gtggttttga gaaggtaatc aatgtacaag
    31321 gtagcgatca tcacggtacg attgcacgtg ttcgctctgg tattcagggg gtagctcaga
    31381 aacgtcattg ggcgattcca accacatatc cagattatgt tttacataag atggtgacag
    31441 tcgttaaaga tggtgcagaa gtaaagctgt ctaagcgcgc tggctcctac gtaaccctca
    31501 gagatttgat tgagtggtct ggtggcgttc atgcgcaaat gtcagtaaca gagcgagagc
    31561 tggcattaca gcgtggacgt gatgcggttc ggtttttctt gatctcacga aaggccgata
    31621 cagaatttgt ttttgatgtg aacttagcgc ttcagcaaaa tgacgaaaat cccgttttct
    31681 atgtgcaata cgcgcacgct cgcatttgct cgattttgca gcagtggggt ggggagcaaa
    31741 ataatctcag cgctgctaat ttagatcttt tggatagcaa agcagcggat catctcttac
    31801 gtcgcttagc agagtatcca gaggtcttag caaacgctgc ccaagattta gctccgcatg
    31861 ccgtagcgtt ttatttgcgg gatttagcag gagatctcca tactttttat aatgcagacc
    31921 gtgttttagt cgatgagctt gatctaaaat tggcccgcct agctttatta tcagcctgtc
    31981 gtcaggtttt agaaaatggc ttaaaagtct taggggtttc agccccaagc aagatgtgat
    32041 ctattgagtg caatggagtg tttagttttt ccatcttgat gcgggtaaga tagagattat
    32101 gaagccatac gaacatcaaa atacggccac acagagcact aattctcatt tactgcgttc
    32161 gtcgcaatcg ggtggcatca tcatgggcgt ggtcattggc ctattggtcg gcgttggcct
    32221 ggcactatta gttgcttggt atttaacaaa atctcagccc caagaaagac ctggagttcg
    32281 tgcgccaagc gcaccatcat ttatgaaacc cgtccctcaa aaggcagaaa gagaagagga
    32341 ggaggagacc tcggtcgccc cacctcaggt cgatcttaat aagcctttac aaggaaagaa
    32401 tgctgggcct ggggtgcgcg atccaattgc tgatatcgct acaggtaaga cccctgatcc
    32461 taagccagag gcgagaggcg atacacagtt ttatgtacaa acaggcgtct ttaatcagcg
    32521 cgcagatgct gatgctcaaa aggctaattt ggcgatgcaa ggtatacaga ctcaactcag
    32581 tgaaagttct gttgaggggg ttacagtttg gagagtacga attggtcctt ttactagcat
    32641 tgaagacact gcaccgacta gaagtaagtt aaccattatt ggcattaaac cgaccgttat
    32701 tcgagttaat aaatcatgat ccatatgttt aaatacctct ccattttttt gatacttttt
    32761 ggtttacagc acggggccgt tgcacaggcg cccgtgaaag tagaagaggg atttgattat
    32821 cgtgtgttaa cgatcaccca acctattgat accaagggta aggtcgaggt tatcgaattc
    32881 ttttggtacg gctgcccaca ttgttatgag tttgagccag agcttaaggg ctggataaag
    32941 cgacaaaata aagacgttgt attcaagaaa gtccccatcg cctttcgtga ggagctaatg
    33001 cctcatagcc agctcttcta tgcattggag tccatgggca aaggcgatgc tttaaatgac
    33061 aaaattatgt ttgcgatgca tcgtgagaac aagcgtctgc ttaatgaaaa tgagattgca
    33121 gattgggtgg cggcccaagg agtggatcga aatgcattct tagctgctta tcgttcgttc
    33181 gccgtcttat caaaagctag ggctgctaat cagttgggca attcttatcg tattgatggg
    33241 gtgccgacgg tcgctgttca gggtaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33301 nnnnnnnnnn nnnnnnnnnn nnnnnngtac gatccaaaaa tatatagggt acggcaggat
    33361 tcggagaaat ttcaggaatc ctgagaagcg tttaggaaag tgaatatcaa attcaccacg
    33421 ccttatacct tctaagatct cattggcagc ctcttccgcc gaaataagag cgggcatctc
    33481 aaagtcattt ttagcagtag cctcggtggc aacaaagcca ggcgagatca tatgcacccc
    33541 aatccctttg ggctgaagat cataaaaaag ggtttcgcag aaattaatca tggctgcttt
    33601 tgtgggccca tacgccagag cttttggtag accgctgtag cctgcaacac tgcccacaat
    33661 ggcaagatgc ccatgcccta cttgaagcat atctggaagc acggtagcta cagcccgcat
    33721 tggacccaga atattggaat caactgtttt ttgagcatct tccatattga agtcatctgc
    33781 tcgcaaggga atatagatgc cagacacaaa aagtaataga tcgatgccac cccattgcgc
    33841 cttaatcgtt tgatnnggcg tgggcaatcg atgatggatc catgacatca actggcacaa
    33901 caagtgaacg attgggatcc gcagattgcg caatggcctc taggcgatca atacgtcgac
    33961 tagacaaggc taattttgcc ccacgcttta ataatgcgcg tgcacacgct tcgccaatac
    34021 cacttgaggc gccaatgacc caaacgcgct gatccgtgaa gtctggaatg gacgcctgtt
    34081 tcatggagcg cccctacttt tttgatggct gagggtgaac tggaccacat cgatattctt
    34141 ttctttaaag cccgccgcac agtaaagcag ataaaaattc cataaccgta taaaagcttc
    34201 atcaaaacca agttctttga tttcagagat cttctgattg aagttgtcaa accacatgca
    34261 cagagttttg gcatagtcct gaccaaatgc aaattccatc tcaatttgaa ggcctgcatt
    34321 attggcatga tgttggaatg cttcccgaga tggcagcata cctccaggaa aaatatactg
    34381 ctgaataaag tcagtacttt tgcgatatcg atcaaataaa ttctcattaa tcacaatcgt
    34441 ctgaatacag gccttgccat cttgttttaa gcaacgtgcc aaggcctcaa agtaactcgg
    34501 ccaatactgc tcaccgacgg cctcgaacat ctcaatcgaa gcaatcccat caaactgatc
    34561 tttacaatct cgataatcct gcattcgtac ttgatgatag ttaggccggt gtcgcgcaag
    34621 tcgtttggag gcatacgctt gctgttcggt cgacagtgtt aagcctgtaa tacggtgacc
    34681 agcatgtgct gctgtttcca taaaacctcc ccagccacaa ccaatctcca gtaattcact
    34741 atttggtgca agatctaagg atttgaggat ggcttgatat ttagcagctt gagcatcctc
    34801 aagtagggac ttttcctgtg cattaaacca tgcgctagag taggtcatgc tgggatctag
    34861 ccacagggta tagaaggcat tacccaaatc atagtgggca tgaatatttt tacgactacc
    34921 cactttactg ttgcgattca aatagtgtcg tatccgatag accaaggacc caaaccaatt
    34981 gccatagata gcctgctcta agacattgcg attttgaact gctaggtcta agagctgttc
    35041 gaggtttggg ctatcccaat ctccccgaat atagccttca gcaaaaccaa tatcaccatg
    35101 cctgaggata tctttaaatg cactccaacg atgaaagcgg atttcagcct ggaggggatc
    35161 ctggggttta ccaaacaccc tcctctcgcc atttgggaaa tacaaggtta ggtagccagc
    35221 ggagagctcc tgtaagagtc gctcaatcgt tctagcagca aacgggatga cggcacccac
    35281 gtccggctca ttagagcgtg agaagttcaa cttcgctaat aaagactggc cagggcgcat
    35341 catgagctaa tttccatctt tggaacatca ggtttggaat gaaatggtac cccttttagc
    35401 caaagtttga gcgcttgcca atgaatccga gcaataaccc caaaagtcat catggggtat
    35461 tggaatttgg cacgtcgtag ggccatcttc gagagtacgt gatcttgacc actaatgctg
    35521 gtgtaaatca ggggcttttg atcaagatgt aactcaatgc ggcatacaga atttgattta
    35581 ctttcactgt ctttggaaaa taaaaagcga aagcgatatt cgccggagac atcgcaaaaa
    35641 ggagaaacat gaaactcttt tttactgatg agggtttctc cagaatacag gggttcacca
    35701 ttttcttttc gtaataaata gcagtgacgt tcgccaaatg tgttgttaac ttctgctaga
    35761 actgcacata ccgaatggtc tgcacgtgta tagatccaaa aactcactgg gttaaagaca
    35821 taacccaaga tccgtggaaa ggtttgtaac caaatttctc catcagggat atggatacct
    35881 tctttggcaa acaaatcttc tacccattcc aagctatcct tctcaccctt gccatggtct
    35941 ttatcataaa aagagtagaa ggcccagtga ttatcaccaa taccaaactg ttccagtagt
    36001 gcaggattac gcttgcgttc acgcatcgga atcttgagag taaacacatc gtaactaaag
    36061 cgattctgag caggacgtaa acgagtgtgc tttacttgac catagttaat gaagggctta
    36121 tacattagtt ggttgattgt aagggcggtc ttgttagggg cgaatggagt gaggcaatca
    36181 gatcagccgc caccagctcc ccagatcgta agccatcctc atgaaagcca tacccagtcc
    36241 atgccccaca ataccaaata ccagatttac cctgaatcaa gggtaagctc ttctgagcgt
    36301 ttatggcagc attattaaaa agaggatgcg catacgagat ctcagcaaaa atcgtttccg
    36361 gtttaggtgc ggtaagtgga ttaagactca caataattgc ttgctcatca agactaaaag
    36421 gtaatggttg taatttatta atgaggtagt ccacactcac ctcatgatca ccaagctgtg
    36481 accctgcggt tttgtagttc caggcagccc agcagcgtct cgttttagga agaacagtgg
    36541 aatcgtggtg caagattgcc ttattttctt gataaggaat cgcttcaaga agggattgat
    36601 caaacgcatc aataccatgc aaaagctcta gactttgatt gctgtgacaa gccatgacaa
    36661 gctcatcaaa ataatgaacg cccttggcgc tgatgagttc aatacgacga tcaatatctt
    36721 ttggactcgg tacattaacc ttaagaactg cttcgttgat taacttaaaa cgatcaggat
    36781 gcatggagtc taagatgcgt ttaacgtact gcttcgaacc gcctttgacg gtgagccact
    36841 gaggcctatt ttgaacttgg attagaccgt gattgtgaca aaaccgaatc atggtctcaa
    36901 taggaaactc gagcatttgt gtagtagggc aagaccaaat cgcaccaatc atgggtaaga
    36961 aataccactc ccgaaatgcc gtcttaaatc cgtgctgatc taaaaatgaa ccaatcgatt
    37021 gtaattttgt agggtccgta ccatgactag agttttgctg ttgcaaagcc aattgcgttg
    37081 ctaattgatt aaagcgcaca atatctttaa tcattcccca aaaggcggga cgggcgagat
    37141 tgagacgttg tccaaagagg gtattgagac tatctccaga ccactctaga gattgatcaa
    37201 tggagaccga aaacgacatc tcggaaagcg caagcggaac ctttagttct tcaaataaac
    37261 gcgtgagacg gggataggtt ttgcgattaa atactaagaa gccggtatca acactacaag
    37321 gaatcctgcc gcgtgctgtt tgtacctcaa tatccactgt attactgtgt ccgccaatat
    37381 gttggccgcc ttcaaataaa gtcacatgaa cgtccgcatt cttttgcaaa gagtaggcac
    37441 aacccagccc agaaatgcca gcaccaagta cagcaatctt cttcatcgat gaatggtttt
    37501 gattcagatg actcacgaag acttaccctt tagcaacttt tcaatttcgg atgtaatcga
    37561 tttgccatcc ggtttggaca agctattgta tgactcgaca atattaccgt tacgatcaat
    37621 taagtattta tagaagttcc attttggact tgtaccggtt ttgctaatca acatcttaaa
    37681 tagtggattt gcatccttac cgctaacaga gcttttggca aacattggaa atttcacatc
    37741 ataggtgttc ttacagaact cggcgatctc tttattgctt ccaggttctt gctgaccaaa
    37801 atcatttgat ggaaaaccca aaatcacaaa ccccttatct ttataacggg cgtaaacttt
    37861 ttctagacct tcgtattgac tagtaaagcc gcaaaaacta gctgtattaa caaccataat
    37921 taccttaccc tggtattggc ataggttttg aggtacctca tcttgtagcc ttaaaaaggt
    37981 atgtgaaagt gctggagcgc aggtggcgct ggctggattt gcattggcat cgcttggatt
    38041 gagcattaga ttaagtacca tcagaggaag aaaaaaaagc catttcataa ggtaagccgt
    38101 cttcgatagg ggagaagctt aaagtctaac gccttctaga atcttatgtt tgcaaaggga
    38161 tttttgagtt tctaggcatt ttactggcag gttctcggat agatcatcta gaatctcaga
    38221 atgacccagc taagtttgaa gccaccagcc tttgagtcaa aggtttgttc cacagaggcc
    38281 agtaatctaa gccagaagct attggctctt cgccaccccc tagtatttac aaacggagtg
    38341 tttgatattc ttcatcgtgg ccatgctagt tatttggcac aagcacgcga gcatggcgct
    38401 agcctagtag tgggtgtgaa ttcggatgca tcggtcaaat tgctaggcaa aggagatgac
    38461 cgaccgataa attcagaagg ggatcgaatg gccttgctgg ctgccttaga aagcgtagat
    38521 ctagtgatct tgttttcaga acagaccccc gttgagttaa ttaaacgagt tcgtcccgat
    38581 gtgtatgtca aaggcggcga ttacgccatt gaagcgctcg aggaaacaaa ggtagtgaaa
    38641 tcatggggtg gtaaagcctt cgccattcca tttatttacg atcgctcaac caccagccta
    38701 ttgggaaaaa tacgctctta ggccgccgtc actacttttt ggttttgacc caagcccaaa
    38761 gaccacgcaa tactagtccc tgtacctttt ctgccttgat attgttttga tgaagtcgcg
    38821 tcaagagagt atctgcatgt ccgccgtcaa tccaaatacg agatataggt tgatttctat
    38881 tttttgcaaa ctctagggcg cccattaatg ccccagtttg cgcgagcaag caaccttcct
    38941 cgatagcgct gaaagtattc aggccgattc cagagctctt tattccatcg tccggaatat
    39001 ctagccttgc ggtattttca atcaagctat ttcgcattaa tgaaatgccc ggaaggatcc
    39061 agccaccata gtgcattcca ttgcttccca aaaagtcaat cgtgcttgcg gttcctgcac
    39121 tcacaatcaa ggtgttatca ctagatagtt ggcgagctgc aatgatggcc gcccaacggt
    39181 ctgcacctag agtttgagtt tgttgatatt gggtgcgtaa ctgagggtat tcagagttac
    39241 cgagtagacg gtctgttgga attcctttcc agtcggccaa gagattttgt agtgactcgt
    39301 aataggattc tgcggctaca caacagatgc caattcgttc tggttgtggg atggtgcttt
    39361 taatatagtg tgcaagttct gcgcgatgct cttctgagtc tagtagtttg gtatcaattt
    39421 ccccagaata agcccacacc tttttctgac gctcgctagg ttcacgatca ttttcaacac
    39481 aagaccactt gagtcgagag ttgccaatat caaaaagaag aagattgctc atgatcgttt
    39541 tttaagggat agatcaccag cataaattac ttttgcaggg catccaggat gctcttccaa
    39601 tagaagtgcc ccctcctgac tgacccctag tgcaatccca gagtattggg tcttaccaaa
    39661 ctcggtaatc tcaactgctt gattttgata agcatcccaa tttttccatt ccgattgaaa
    39721 gggggcaaaa ccctctcggt taaatacatc taaatagttc cctagggcat caagcaagtt
    39781 gagccaaact aaatcagctt ctattcttac ctgcttcgtg ttgcattgcg ctagagcggc
    39841 aattgggatc tgactagact gttcgatctc agtagagtgc tctagattga tgccaacccc
    39901 aatgatcatc catgtttttt cagtggggct tgatgaggca agtttgccac cttcaattaa
    39961 tagccccccc agcttctttc catgaatcag tagatcattg ggccatttaa gaccaacgcc
    40021 caggctatgc aatttttctt cgggcaattg caaactattt gcaatccctt tgatcgcagc
    40081 caagccgcaa actaaactta agccatgaag accttgacta tcgcgtgcaa agggataggc
    40141 cagggagaag catagagagc tttgcgggtt agcaaaccag cgcctacccg ccctgccctt
    40201 acctttcgtt tgatggcgcg ctagacggga gactggatca atgagctccc ctgctcgcca
    40261 gcgggccatt aaatcttcat tcgtagactc ggtttgacta acgcgctcga ggatgcagtt
    40321 ccagttcatt tgtatattgt agaaaggtct agatagaatg ggtacttatg ttcaataagc
    40381 tcagaaaaga ccctgttaaa aagggtgacg attaagtgcg agagccacat cgcctgaatt
    40441 ggcctttaca agcaatgcct ctggcgcgtg ctattgcgat cgtctatacc ttgttaattt
    40501 tctatggttg cctaaacccg tttaattttg atcttaattt tggtttagac ccctttgcgt
    40561 ggtggtatgg ccctttgccg aagtacatca cctcatttga tatgaccgcc aatgtattag
    40621 gttatatacc cttgggcttc gtattggtgt ttgcggttta tccacgatgg caaggcttag
    40681 cggctgtggc cattgccctg atatatagcg gcttagtatc ggcgtctatc gagagcgcac
    40741 aaacttggct acctacccgt gtgccgactc aaatggattg gttcgcaaat atgttgggag
    40801 catttattgg ggcgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40861 nnnnnnnnnn nnntttgcac agatttatcc acaaagctca tggttgggaa tgggcttctt
    40921 aagtttagga cccgatcttt ctcctaattg gggtacgcaa gtgattaatc acacgattca
    40981 ggaggggctc atcacgggtc taagttggct gggagtcgga ctgttcttct ctttggcatt
    41041 gcggcccaat gtatccaagt acaaactcct tatggcactc ttactggtga ccattctcat
    41101 taaatcctta tttacggcct tacagtttgg tgccgagtat ggttttgtat ggctcagtgt
    41161 gggcgctatc tggggcatgg ttttggggag cctatttata ttaataggcc tagagttatc
    41221 cgcacgcatc cgcctaatta cctgtatcac tgcctttatt ggtcttatgg cactggtcaa
    41281 ctattttccg gataatccat attttgcaat tagtctaccc cgcttaaatc aaagtcgttt
    41341 agtgcacttc aacgatttga tgcaatggct ttcttggcta tggttgccag tagccttgat
    41401 atggctggtt cactacgcta ggggcgttaa acgctagaaa gttacccgaa ttacattcca
    41461 tcgatataat tttgtaatga gctatcaata ccacctattt ttctgtttga atgatcgcag
    41521 caacggagat gattgttgca ttcgtcataa ctcccaagcc ctttttgact ttactaagaa
    41581 acgcgttaaa gaattaggct tgaatggcag cggtaaggtt cgtgtcaata aggcaggttg
    41641 cctagaccat tgcgccaatg gcccagtgat ggtggtctat ccacaaggca cttggtattc
    41701 catgattgat actgaagata tagaagaaat tattcaatct catctagtgc agggtaagcc
    41761 cgttgagcgc ttattgatct agccatattt gagcatgcta aatgctctat gcatctatga
    41821 atagccgcac tcacttgatt actgttccgg gtccagcggg cctgattcag ttatcagtcg
    41881 acctacccga tgagctcaaa caagacccac attttgcggt acgcggctta gctttaattg
    41941 cacacccgca ccctttgttg ggcggcacga tggataacaa ggtcgtacaa accttggcaa
    42001 gaacatttaa tcagctcggc tatgtcagtg tgcggcctaa cttcagaggg gtgggcttat
    42061 cagcaggcac ccatgatgag ggccggggag agctagacga tcttttgatg atcagcgatt
    42121 ggatgcgaac cccacaatca tggttatctc atttaaatca ggctagtgag caagcatgga
    42181 tcactaaaac taatcaactg ccactcgtat tggccgggtt ctcgttcgga agttatgttg
    42241 gtagtcattt agtacatcaa cttggcgagc tcggtaagcc cgtcgagcga tttgtgatgg
    42301 ttggtagtgc tgctggtaaa tggaagttag cggattctgt acctactgat actatcgtta
    42361 ttcatggtga gaacgatgag accattccat tgttggcggt atttgattgg gctagacctc
    42421 aggagttaac ggttcatgtc gtcccaggcg cggaccattt tttccatcgc aaattacact
    42481 gtattcgaaa cattattttg tcgcattggt taggccaagt ttcgtaataa tctattcatg
    42541 aactcaaaag aagcccccga atcactcatt cagtaccctt gcgattttcc gattaaggtg
    42601 atgggaaagg ctaccccaga gtttttacca gcagtagtgc atattgcaaa acaatttgat
    42661 tcgggttttc aagaaggaac catagagcag cgcccatcgc gtgacgcaaa ttatttaggt
    42721 ctcacgatta cagtccatgt tcatagccgc gagcaattag atgaaattta tcgaacgctc
    42781 tcaacgcacc ccttagtgag cgtggtactt taagtggatc tctaggcata cgatgcaaat
    42841 aagtccagta ttgatcaaaa atttgggact tgttgactat caggagacct atgaagcaat
    42901 gcagcgcttt accaaagagc gcttacctga tactcccgat gagctttggg ttctgcaaca
    42961 ccctcctgta tttactttgg gccttgcggg caacccagct aatcttcatc aaccaattgc
    43021 gcacatccca ttacttgagg ttgatcgcgg tggcgagatt acctatcacg gtccaggtca
    43081 aattgttatt tatctccttc tggatttaaa gcgcaagacg ctttttgtga aagaattagt
    43141 tagcgctatt gaacaatctg tgattgatgc cttagctgga tatggggtga tggctgagcg
    43201 caagccagga gcgccaggaa tttatattgc caagcaaaac cagcccacct tatatgaggg
    43261 tgcaaagatc gcggcgcttg gcctaaagat cactaagcaa tgcagttatc atggactagc
    43321 tctcaatgtg gtgatggatt taggcccttt tggggcaatc cacccttgtg gatatgaggg
    43381 tttgcagacc gttgatatga aaactcttgg aatcagcgag aatatcgact tagtcgccgc
    43441 gcgcttagta gaccatttaa aaaggcaatt gcatcttgca ccagtgttat gagtaatcaa
    43501 aaaaaagaat acgatccgct gcaaaaacaa aagtcagccg acaagaccgc tcgcattcca
    43561 atcaaaatag ttcctctggc agagattctt aaaaagcctg attggattcg ggttaaggct
    43621 gcctccagca gttcacgatt tacagagatt aaaaagatct tgcgcgaaaa ccagttggtc
    43681 acagtatgtg aagaagcgag ttgccctaat attggtgagt gctttggtaa gggtacggca
    43741 acttttatga tcatgggcga taagtgcaca aggcgatgcc ctttctgtga tgttggacat
    43801 ggcaggccag atcccttgga tcagaatgag cctgttaact tagccaagac gattgctgct
    43861 ttaaaacttt catatgtcgt gatcacgagc gtagaccgtg atgatttgcg tgatggtggc
    43921 gcacaacatt ttgtagactg tattactcag accaagaaac tttctcccgc aaccagggtt
    43981 gaggtattgg ttcccgattt tcgggggcgt ttagagaaag cgcttgacgt ttttgcgagt
    44041 gatccattgg gtcttcccga tgtgatgaat cacaatctag aaaccgtccc gcgtctttat
    44101 aaacaagctc gaccaggatc ggattatgct cattccttaa agttgctaaa agattttaag
    44161 gggcgctttc ctcacatacc aacaaaaagt ggtctgatgg tcggtttggg tgagaccgat
    44221 gcagaaattt tggatgtgat gcgcgatatg cgtgagcatg atattgatat gttaacgatt
    44281 ggtcagtatc tatccccctc caatcaccac ctccctgtgc tgcgctacgt tcatccagat
    44341 gtctttcaag agttcgaaga gaaggcttat caaatgggct ttagtcatgc agccgttggt
    44401 gcaatggtgc gctctagcta ccatgctgat caacaagcgc atgaggctgg tgttgtttag
    44461 gccctatcaa cagcttctta cactagccct ttgtttctgc tttgcattta ctttgttggc
    44521 gtgcagtcca aaattagatt ggcggaccgt taaaaatgag tctcttggct acgctgcctt
    44581 tttccctgga aagccccaat taattgaccg tagcttgagc tatcaagaga acaaacttaa
    44641 acagtctctt gagttcgtta aagtggatga cgatatcttt gccgtgatga ccactgaaat
    44701 ccctgtggct ctcgcaaatc aagtttcggc gattgagcag ttactaaagt cggttctttt
    44761 acagcagact ttatctccca atcaaagcac cccatcagaa tttaatcggg tgaaagaaag
    44821 tagctccaaa ataggtgcta ttggtcaaca gaaacaagca agtcaggatt attttttaga
    44881 tttacctcaa gctaaacgag tgatgcggat tcggtggttg atacgaccca tggcagacgg
    44941 agggctttat ttgtatcaag ttagtgtagt gcgggctcaa acaccttcaa atcccatgcc
    45001 ctccttagag caagagaata tcaatatctt ttttgatgag ttccggccaa attaagaaaa
    45061 tgagtaaagc gcattactcg cactggtatc gacagcccga ccagcactta gctctgccac
    45121 tgcagggtac atttgccttg cgtgtatttc tgtgttttgc atttgcctac tttatgtcnn
    45181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45241 nnnaacaatg ccgatttggg tcttctttcc tccgcctact ttatcggttt tggcgctact
    45301 caaattccgc ttgggctggc cctggatcgt tttggccccc gcattacaga gatatgggtg
    45361 atgaccctgg cggtcgtcgg cgcactgata tttgccattg ctgaggattt cacaacgctg
    45421 gtgatggcca gagtactcat tggcatgggc gtctcagcat gtttaatggc gtcattttcg
    45481 ggctttcggg cttggtatgc gccagcacaa caggggcaat tagcgtctgc catgctggta
    45541 tttgggacca gcggtgctct agccagtaca tggcctgtcc acttagtgac cccttacatc
    45601 ggttggcgtg gcgtattttt agtgatggct ggcttaacat ttttagccat tctgactttg
    45661 tattttggtt taccagttag aaagtccgcc tccgttgaga ggcaggcgta tcgatcatct
    45721 acaaacttat cgtggcaaag ctataaacca atcttgacca atgcattttt ctggcgcatt
    45781 ttgcctttgg gtacattttg ttatggcggc tttattgcga ttcaaacact atggtttggc
    45841 ccctggttaa tcgaggtgat ggactaccca ccaacaactg cagcacaaat cgtatttggg
    45901 ttcaatgttg ttttactgct cgcctatctc tttaatgcct gggttttacc aaagttagct
    45961 cgcgttggaa tcgatacgat gcgctacatg acatggatgg ttggcgtctc gatgattatg
    46021 caagcgtgct cgtatttttg gcaaacatct ttcgtttgga tttggtggta tctgtttgcg
    46081 attacctgcg cctcttttgt tttggctcaa agtattattg ttttgtattt tccgaagcat
    46141 tactcaggac gagtaagcac tacctataac ctcacactat ttattggcgc attcatcgtg
    46201 cagtggggta ttgggcatat gctcgactt
//
LOCUS       DDPQ01000094           21586 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_1086,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000094 DDPQ01000000
VERSION     DDPQ01000094.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 21586)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21586)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21586
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_1086"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    7349..7369
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5711 a         4709 c         5256 g         5889 t
ORIGIN      
        1 aagcgtgtat ttattgcttt gcatatgcac gcaggtgatg gcaatgtgca taccaatatt
       61 ccggtgaact ctgatgatta tgaaatgcta caagatgccc atcgctcggt tgatcgcatt
      121 atggccttgg cgcgatcgct tgatggggtg atctcaggcg agcatggaat cggaattact
      181 aaattagagt acttgagcga tgaggagcta aaagactttc gttcatataa gcagcgagtt
      241 gatccaaatg ggcatttcaa taaaggtaag ttaatgccca atgctaattt acaaatggcc
      301 tacacaccaa gttttggctt aatggggcat gagtcattga tcatgcagca aagcgatatt
      361 ggtgcgattg ccgatagcgt gaaggattgt ctacgctgtg gtaaatgtaa gcctgtttgt
      421 gcaacccacg taccgcgagc caacttactc tatagtcctc gcgataagat tttggcaacc
      481 tctttattaa tagaagcgtt tttatatgag gaacaaactc gccgtggcat ctcggtacgc
      541 cattgggaaa tgtttgatga cgtagcagcg cactgcaccg tttgccataa gtgctatacc
      601 ccctgcccag tgaaaattga ttttggtgat gtcaccatga acatgcgcaa cctgttgcgc
      661 aagatggggc agcgtcgttt taatcctgga acggcagcct ccatgttttt cttgaatgca
      721 agcaatcccg agaccattcg tttaacccgg aaagtaatga ttgagtgggg ttatggcatt
      781 cagcgctttg cccatcaatt actcaagaag ttagctaaac aacaaacaaa gcgacccccc
      841 gccactgtgg gtaagccgcc catccaagag caagttattt actttattaa taagaagatg
      901 cccggtaatt taccaaagaa aacggcaagg gcattactgg atattgagaa tgcggatttc
      961 gtaccgatta ttcgtgatcc ccagaaaacc agcgctgata cggaggcagt tttttatttc
     1021 ccaggctgcg gctctgagcg tttattttct caggtcggct tagcaaccca agccatgctt
     1081 tggcatgctg gtgtacaaac cattttgccc cccggttact tgtgttgcgg ttatccccaa
     1141 aaagggaatg gtgactttga taaagcagaa aaaatgatca ccgataaccg tgttttattc
     1201 catcgtgtgg ctaatacctt gaactatctc gatatcaaga cggtcgtggt ctcctgcggt
     1261 acgtgttatg accagttggc cggctatcaa tttgaacaga tcttccctgg ttgccggatt
     1321 attgatattc atgagtacct gttagaaaag ggcatcaaat tagagggtgt gaacggcgtt
     1381 cgctacatgt accacgatcc ttgccatacc ccgatgaagt tacaagatcc cttaaagact
     1441 gtgaatgagt taattcagac tcaagatggc gctgtaatta ctaagaacga tcgttgttgt
     1501 ggtgaatcag gaaccttggc ggtcacgcgc ccagatatat ctacccaagt gcgctttcgg
     1561 aagcaaatcg agatggagaa aggtgcaact gccctacgtc aagattttgc gggtgatgta
     1621 aaggttttaa ccagctgccc atcttgtttg caaggcttaa gccgttttga tgctgatagt
     1681 aaaaccagcg cagactatat tgtggttgag atggcaaagc atattttggg ggacaattgg
     1741 atgcaagagt atgttgccaa agcaaatcaa ggcggcattg aaagggtatt ggtttaaatg
     1801 attccaacaa atgtaatcgt tcatgagacc tcaaaggtct tggagcttgc ttatgagaat
     1861 ggcaagaact atcgcttacc gtttgagttt ttgcgggttt actccccatc ggcagaggtg
     1921 aggggtcacg gtcctggaca ggaggtgctg caaacaggta agcgagaggt ggccattgtt
     1981 aacctagagc ccgttggtca ttacgcgctc aagcctacct tttcggatgg gcatgattcc
     2041 ggtttgtact cttgggagta tttgcatgat ttgtgtgagc atcaagatga gctctggaac
     2101 gactatttgc aacgcctcga aagtgctggg gctaatcggg atgttcccat ggtgagtcca
     2161 gcaaagggct gcggccatta acgaatagca catcaagcac ctatgtccca aactcacttt
     2221 ggctatcaaa gcgtagacga aaaagaaaaa gccgagaagg ttgctgaggt tttccattct
     2281 gtcgccatga aatacgacgt catgaacgat ttgatgtcgc tcggcatgca tcgcttatgg
     2341 aaaaaattta ctattgcgca agctaatgtt cgccccgggc aaaaggtgtt ggatattgcc
     2401 ggtggtactg gcgatcttgc cgccgccttt gccaaggcca cgcagtgggg cgagcaccca
     2461 gatgccgagg tttggctcag tgatatcaat gcctcgatgt tgggggtggg acgtgatcgt
     2521 ttattggacc ggggcttggc ccttccagct attcaatttg atgccgaaaa aataccattc
     2581 ccaaataatt attttgatgt ggtgagcgtt gcttttggtc tccggaatat gacccacaaa
     2641 gaggctgcat tgggcgagat ggctcgggtg attaaagcag gcggtaaggt tcttgtgttg
     2701 gagttctcaa aaccagatgc catgatcgcc cccatttacg atatttactc atttaaggtg
     2761 ctgccatggc ttggcgataa ggtggctaaa gacgctgcga gctatcagta ccttgcagaa
     2821 tcaatccgga tgcacccaga tgcagacgca ctcaaaacaa tgatgttggg ggtcggtttt
     2881 gatcaggtag atacccatag gatgactggg ggtatcgtcg ctttgcacat tggtattaaa
     2941 tattgagcat tattagtaaa ttagttatat attgtgattc actaggaggc cgtagcatat
     3001 gaaaaacatc tttttgaaag ccggtgtatt tacgattgcc ctgacattta tgtcactgat
     3061 gcatgtaagt accgtagacg ctaaacgttt gggtaatggt actaatgcgg gccgtagttc
     3121 gaatgcgccc atgcaaaagc aagcacaacc tactgcccag caaaaaccag tacaagcaca
     3181 acaacctgct gctgcaccgc aagctgcagc ccctgcggca gcagccaaac gctctggtat
     3241 cggtggcatg cttggcggct tagccgctgg tcttggaatt gcatacttat tgtcccactt
     3301 aggtttgggt gaggcagcct cttccttctt aatgggcctc ttattagctg tggcagttgg
     3361 tattgcactg ctctttatcc tcagacgttt tatgccagct agtgcaaaga gcactcctga
     3421 gccggttgct gttcctgccg gcatgcaacg tacagcctac caggaaccca gctttaatcc
     3481 gcaagcaagc aatcagtttg gtacacaaag ccctgtaatt gaagagcgtc ctgcatatca
     3541 gctacccgaa ggctttgatc aaattgcatt cttggccaat gcaaaacatt actttacgcg
     3601 cctccaaaaa gcatgggact ctggagattt agaggccttg cgcgagttca caactcctga
     3661 gatgtttgcc agcttgcaac gcgatcttca gggtcgtgcc gaaggtgcta atcaaaccga
     3721 tgtagttacc ctcaacgcag aattgctcgg ggtcgaaaca gatgccaaca cctatttgtg
     3781 tagcgtccag ttctctggaa tgattcggga gcaggctgat gccccggctg agccatttac
     3841 cgaaatttgg aacttgtcta agcctgttaa tgggcctgga ggttgggttt tggctgggat
     3901 ccagcaattg gtttaagctt ttacctttcc ccaacggaaa acccgccttg tgcgggtttt
     3961 ttacactatg aacccgaact ttgatttgcc gaaggcgcta ttagtgcgcg gcattaatca
     4021 cgtcttacag cgtgaagaat gggcattacg ggatttaaag aagcatgatg ggaaaattat
     4081 tcgtttggtg tttccagtta gcgaaacctg gatacaaatt aatgcaagtg gccatattga
     4141 tttgttgggt acggaagttg agagcgctca tctcacgctc gaggtttcaa atgaatcttt
     4201 aacgcggatt agtgaggcga gtggcagctt tcaggaacgg gtagttaaag cggtcaaaat
     4261 tgcaggggat gctgaactgg ctcaattgat tgccaaattg gcaggccagc ttcgttggga
     4321 gtatgaagag gatttggctc aagtgattgg ggatgcgccc gcgcacttta ttgtttctca
     4381 ggccaagcgc tttcatgaac tgactcagaa agctatttta gatttgcagc aaaatgtgat
     4441 tgagtacttg agcgaggaaa aaaaagtttt agtacatcaa cgtgaatttc tctcacataa
     4501 aatagcgttg caagatctgc gagatgcggt tgagcgtttg gaaaaacgca ttggccattt
     4561 acaaaaaagt caggattcat tgtgagccgt atagcccgcc tcagtattat tttctacact
     4621 gcttggcgct ttaacttatt tggtttaatt tgtgacaacc taaagcctgg tgtacgcaag
     4681 acattgctcg ggcttttatc aagcacatcc ccaagtcaat tagcccgggg tgagcgcatt
     4741 cgtttagcct tagaggcctt gggccctatt tttgtaaagt ttggtcaggt tctctctacg
     4801 cgtcgagacc tattgccaga tgatgtttct aatgaattag ccaaattaca agatcaagtc
     4861 ccaccatttt ctaacgagca atcaaaagca attattgaag ctgctcttgg ccagcctatt
     4921 gagcatacat tttctttatt tgatgcgacc ccggtagcaa gcgcatcggt tgcgcaggtt
     4981 cactttgggg tattgcgaga aacagattct catcctgaat gggctaatca ggaagtggcc
     5041 atcaaagtat tacgccctgg cattttgccg gtgattgaaa gtgacctggc actgatgcac
     5101 gacttagcat gcattgttga aaagatttca gaggatggta gacggctgaa gccacgtgaa
     5161 gtggtcgcag aatttgattc ctacttgcac gatgagttag atctgatgcg tgaggcggca
     5221 aatgctagtc aactacgccg taactttgca gattctgaca agctcatgat cccagaaatg
     5281 atttgggatt tatgccatac caatgtgatt gtgatggaac gcatgtacgg gatttcgatt
     5341 ggcaagacca aagaattacg agcagcaggc gttgatttca agaagttagc agtcgatggt
     5401 gtggaaatat tctttactca agtattcgag tatgggttct tccatgccga tatgcatcca
     5461 ggcaatattc tggtgagctt agatcccaaa acctttggtc gctacatctc gctcgatttt
     5521 ggaattgttg gcaatctcag tgagtttgat aaaaattatc tagcgcaaaa tttcttagca
     5581 ttctttaatc gtgactatcg acgagttgct gagctccaca ttgaatcagg ctgggttcca
     5641 gaaaatacgc gcttagaaga gttggagggc gcggttcgaa ccgtatgcga accatatttc
     5701 gatcgtccgc ttaaagatat ttctttaggt attgtcttga tgcgtttatt tcagacctca
     5761 agacgcttta aggtggaaat tcaaccacaa cttacgctct tacaaaaaac gctattaaat
     5821 gttgagggcc ttggtcgtca gcttgaccca gatttggacc tatggaagac agcaaagcca
     5881 attttggagc gctgggttag tcaacaactt ggctggcggg gtttggtgga tggattaaag
     5941 acagaggcac ccaattgggc aaaaattttg cccacgctcc cgcgtttgct agcggaaagt
     6001 ttagagcgat ctagtcgtaa ggactctcgg ccggagctcg accttctcaa gcaacttctt
     6061 tttgaagagc gccgtaaacg gcgtcaacta tggggctcgc tgctatttct gggggggttc
     6121 ttgctcgggg ctttatttgt tttggctcgc atccattcaa tttaagtaaa tttcgactga
     6181 ttttgagcta agtcgctaaa atagtgggtt aggcaggaaa tcatgaaaaa atactttatc
     6241 gcaggcattc tagtttggat tccaatagca atcactgttt gggtgattac ttgggggcta
     6301 ggcttgatcg accaaattta tggttcggtt atgagcgccc tcattactgt cctgccaggt
     6361 caattttcgg gggatctaaa gcattttcgt gaaatacctg gccttggcat tctgattgtt
     6421 ttggtagtca tcagttttac aggattattg gcgattagtt ttgctgggca gtggtggttg
     6481 aaggtatggg accgttttgt tacccgcatt cccattgtga gatcaattta ttcaagtgtg
     6541 aagcaggtct cttccactat tttttctgga aacgggcaag cctttcgtaa ggctctgttg
     6601 atccgttatc cccatgctga atcttgggta atcgcatttc agacagggaa tacggcagag
     6661 gaaattgggc agaaattggg gggtgagtat gtcaatgtat ttttaccaac cacaccaaat
     6721 ccaacgtcgg ggttttttat gatcgtcccg cgttctgctg ttatagagct cgatatgagt
     6781 gtcgaagaag ctctgaaaca tattgtctcg atgggctctg tgcctccacg ggtatcggca
     6841 agccctatca aaaatcaagt aaatcatctt aattaggaat acttatgtcg atgcgcagcc
     6901 atacctgtgg gcaggtaact gattcgttaa ttggacaaga aattaccttg gcgggttggg
     6961 ttaaccgtcg tcgtgatcat ggtggtgtaa tttttattga tctgcgcgat caccaaggct
     7021 ttgttcaagt agtttgtgat ccagatcgcc cagatatgtt taagcaggcg gaagaagtgc
     7081 gcaatgagtt ttgtattcag ataaaaggct tggtcagggc tcgacctgcc ggcactgaga
     7141 actcagattt aattagtgga aaagtagaaa tactttgtca cgagcttcag gttcttaacg
     7201 cctctgttac cccgccattc cagttggatg atgacaatct ctcagagact actcgcctaa
     7261 ctcatcgggt cttagattta cgtcgcccac aaatgcagaa gaatttgcgt ttacgctacc
     7321 aagttgcgat ggaaactcgg cgctatttnn nnnnnnnnnn nnnnnnnnng gcgcccgtga
     7381 ttatttagtg ccatcccgtg tgcatgatgg ccagttcttt gcattgccac agtcgccgca
     7441 gctctttaag cagctcttga tggttgcagg ttttgatcgc tactatcaga tcacaaagtg
     7501 ctttcgtgat gaagacttac gcgccgatcg gcaaccagag tttacgcaga tcgattgtga
     7561 gactgcattc ttggatgaga tggaaatccg cgacttattt gagaccatga tgcgtcacat
     7621 ttttaagaca gtgatgagcg ttgacttacc caatcccttc ttgatgatgc cgtattctga
     7681 ggcgatggca cgctttggat cagataagcc tgatttacgt gtagcaatgg aatttacaga
     7741 actcactgac ttgatgcgcg atgttgactt caaggtattc tcaggcccag ccaatcaaga
     7801 aggtggtcgc gtagtcgctc tctgcgttcc tggcggcgct gaaataagtc gtagcgaaat
     7861 tgatgactac acacaatttg tatctattta tggtgccaag ggtttagcgt ggattaaagt
     7921 taattctgtt gctgagggtc gtaatggcct tcaatcccca attgtcaaga atttgcatga
     7981 tgctgcaatt gaaggaatct taaagcgcac taaggccaag gatggcgatc tcattttctt
     8041 tggtgctgat aaaacaaaag tagtgaatga tgcaatcggt aatttgcgct tacggattgg
     8101 ccattctgat tggggcaaga agcatggctt gatggtcgag ggttggaagc ctttgtgggt
     8161 agttgatttc ccgatgtttg attatgacga agatgatgct cgttgggttg cctgccacca
     8221 tccctttaca agtccgaaag atgagcacct gaagtttctg gaaactgatc ccggtaaatg
     8281 tttagcaaaa gcttacgata tggtcttaaa cggtagtgaa attgggggtg gttccgtacg
     8341 tatccatcaa gaaactgttc aaagccaagt atttagggct ctaaaaattg gtcctgagga
     8401 agcggcagct aaatttggat tcttgctcga tgcccttcag tacggcgcac ctcctcatgg
     8461 cggtattgcg tttggtttag accgaattgt gaccatgatg accggagctg aatcgatccg
     8521 cgacgtgatt gctttcccta aaacacaacg cgcccagtgc ctcttaacgc aagcaccaag
     8581 ccctgttgat gaaagacagt tgcgtgaatt gcatattcgt ttgcgcaata ccaatccggt
     8641 cagttaattt gaaaataccg atttcagttt tagtacttat tcataatccg aatcgtgagg
     8701 tcttattaat tgagcgtgca gatcgcccag atttttggca atcagtaacg ggtagcttag
     8761 ataccccaga agagactttg gacagtgcag cggttcgtga ggttgctgaa gagactggca
     8821 tagaggttca tgcactagca agaggttctc tcaaaaattt acatcactat gtggagtatg
     8881 agatttatcc aacatggcgc caccgttatc ctccgggtat agagcgcaat accgagcatt
     8941 ggttcgcatt agaagttcct gtggggacgc cagttcactt ggctcccaga gagcatctca
     9001 attatctttg gctccccttt gaagaggcag cccagaaatg tttttcacag agcaatcgag
     9061 acgcaatcct aaagtttttc acccagccga atgatttaat gggtcgaagc gaggacggca
     9121 ttcaatcatg atggggcatc gacaaggcag ctctagtccg ctacctggaa gtggcggcgc
     9181 atcacgttct gattggcggg tgattaaaga tctattgccc tatctactgg agcaccgaac
     9241 acgagtcgcc ctggcaattg catgtttaat tgcagccaag tttgccaatc ttggcgtccc
     9301 aattattctg aaagatttaa ttgatgcaat gaatctcaaa cccggttcgt tgcaggtcta
     9361 tttaattgtg ccagcaggtg tgattgttgc ttatggagcg ttgcgactat ctgcttcctt
     9421 attctcagaa ttacgcgaac tcttatttgc taaggttacc caagatgcag tgcgtaaaat
     9481 tgctttacag gtttttgaac atctccatgc cttaacactt cagtttcatt taggtcgtca
     9541 gactgggggg gtcagtcgag atatcgagcg tggtactcgc gggatccaaa ccttggtgtc
     9601 gttttcgctc tatagcattt tgccaacaat cattgagttt gtcctggtac tggcttactt
     9661 cgcttattac tacgatattt ggtttgcgat tattactttc gtagcacttg tgatctatat
     9721 cgtattcacc atcaaggtga ccgagtggcg aacacactac cgccgtacca tgaatgaaat
     9781 ggactctcgc gctaatcaac gagcgattga ttctttgttg aactttgaaa ccgtgaagta
     9841 ttttggaaac gaagactttg aatcaaagcg ttacgatgaa aaccttaagc attatcaatc
     9901 ggctgctatt ttgtcgcaaa agtcattagc aatccttaat ttaggacagc aggccattat
     9961 tgcggttggt ttagttttaa tcttatggag agctacccaa ggtgttgtag atggctcaat
    10021 gaccttaggt gatttggtgc ttgtgaacac tctcatgatt cagctctata tcccacttaa
    10081 ctttttaggg gttatttatc gtgagatgaa gcaatcgatt accgatatgg atcgcatgtt
    10141 tgcattactc agcgcagatc aagaaatcaa agatcgggag aatgcacctg cactggtgat
    10201 ctacgatttt gctcggggac ctgaagtgcg ttttgagaac gtgtactttt cttataacgc
    10261 taagcgtgag atattaaagg atgtaagttt tacgattccg gcgggaacga ttacagcagt
    10321 agtcggtcaa agtggtgctg gcaagagcac cttagcccgt ttgctattcc gtttttatga
    10381 tgttcaatct ggaatgatcc tggtcgatgg tcaaaatatt caggatgttc agcaggcgag
    10441 cttacgtaaa gcaatcggca tcgttccaca agataccgtt ttatttaatg acactattgc
    10501 atacaacatt gcttatggca gcccaggcgc tagcctagaa gcaattcaag aagccgctcg
    10561 tgccgcacag atgagccaat ttattgagca tttgccagaa gggtatgaaa cgcaagtggg
    10621 cgaacgggga ttaaagctat caggcgggga gaagcagcgg gttgcaattg ctagaacact
    10681 ccttaaaaaa cctgctttac taatctttga tgaagcgacc tcagccctgg actctaaaac
    10741 agagcgggca tttcaggagg agctctttaa tctggcacgt aatcgcacga ccttaattat
    10801 tgctcaccga ctttccacgg tggtgcatgc ggaccagatt ttggtaatgg atcaaggcaa
    10861 aatcattgag cgtggtaccc atcagcacct gctacaggcc aatggtcgtt acacctcaat
    10921 gtggcagatg caggagagca gtctaagtgc ccccgatgat tcaggtgtta attcagctta
    10981 aaattagggc atgtcaccag gttcctcccc ttcaaatccg cgcacgctac tagaaggggc
    11041 atttgcggca gcattggccg tagctgaccc taaaaagata gtgcctgagt atttggctaa
    11101 agtcttttca cacgggaatg agccaaaagg acgctgcttg gtggtgggcg caggaaaggc
    11161 cagtgcgtcg atggctaccg cgcttgagac ctatgccaag caatattggc cagcgattgc
    11221 ccttgatggt gtggtcctca cccgatatgg gcacggatca ccgacaagtc atatcaaaat
    11281 tgttgaggct gggcatccag ttccagatca ggcgggcatg gatggcgcag ctgagatatt
    11341 gaaattagtt aatcaactca atcccggcga cattcttatt gcccttgtgt ctggaggagg
    11401 atcaagcctt ttaaccttgc cacagactgg catcacgatt gcagatatgc ggaaaaccac
    11461 tgaagccctt cttcatagcg gtgcaccaat tgaagaaatg aatgtggtcc gcaaacatct
    11521 atccgctatc ttggggggta atttagctcg ggctgctttg gcacgtggtg cccgggttga
    11581 ggccttctta atttcagatg tcacgggtga tgatccggct gatattgcta gtggcccctg
    11641 tgcacctgat tactcaagct atatagatgc cttacaaata ttagcaaagt atggattaaa
    11701 tgagagcaag ataccttcat cggtcatcga tcatttgcaa aagggcaaac gtggagaaat
    11761 cccggagacc ctcaagcaaa ccgatatcga caaacaccag tcgccagtac gtaatcatgt
    11821 gattgctaca gcacatcgga gtttggaggc tgccgcagac ttttgcaaac aatctggtta
    11881 caagcccatt attttggggg ataccattac tggtgaggct cgcgatgtgg gcttggtgca
    11941 tgcagcgatt gcccgcgagc tagtgcaata tggagagtgg tcaaagccac cgcttgcttt
    12001 aatttctggg ggtgaatgta ccgtaacgct accgtctggc acaaaaggca ggggtggccg
    12061 ttgcagcgag tttttgctct ccttatttgc tacaagctcc gatctaaaaa ccctgagcgc
    12121 ccttgcagcc gataccgatg gtatcgacgg tagtgaaaag aatgctggag catggtttac
    12181 acccgatatt cgtgagactg ccaaaacgat tcaattacgt gctagccaat ttcaacaaga
    12241 gcatgattgt tacggtttct ttgctaagct agacgccttg gttcatactg gcccaacctt
    12301 aaccaatgtg aatgatttcc gaatcatttt gatcgattga gatgagtaag aaactgacaa
    12361 ttattcgtcc cgatgactgg catttgcatg tgcgcgatgg tgcggtgctg aaagatgtat
    12421 tggcacatac gaccaaacaa tttgcgcgcg cagtcatcat gccaaactta aagccacccg
    12481 tgactacggt tgatttagcg aaggcctatc gttcgcgcat agatactgcg ttacagcagt
    12541 taggagtatc gaacttcaat ccattgatga cactgtattt gacagataac accagtgcag
    12601 atgaggtacg caaagccagc gaacaccaga ttattggatt taagttatat ccagcaggcg
    12661 caaccaccaa cagtgatgca ggtgttacta acattaaaca cgcttaccct gctttagaag
    12721 ccatgcaaaa gcattcggtg cctttattaa ttcatggtga agttactgaa tctgctattg
    12781 atatctttga tcgagaggcc gtatttattg atcgaatact agagccagta cgaaaagatt
    12841 ttccagagct tcgtatcatt tttgagcaca tcacaaccaa acaagcagct cactatgtac
    12901 gcgacgctga cactaaaggc aaaaatacct tgggcgcaac gatcaccgct catcatcttt
    12961 taatgaaccg taatagtttg ttcactggcg gtattcgacc acatcattat tgtttgccaa
    13021 tcttaaagcg tgaagaacat cggcttgcgc taatggaagt ggcaaccagt ggcaacccac
    13081 gatttttctt ggggaccgat agtgccccac atccaaaagg tgaaaaagaa agtagctgtg
    13141 gttgtgctgg ttgctataca gcacacaatg caatgggttt gtatgcagaa gcgtttgata
    13201 gcgttggtca acttgaccga cttgagggct ttgccagttt ctttggcccc gatttttatt
    13261 ctttacctcg taatcaggat caaatcaccc tagttcagca ggttgaagag gtgccttttg
    13321 agttcactct aggcaatacc gtactggtcc cattgcgcgc tggcgagacc attgcatggt
    13381 ctctagcaaa gtaattttta actcaaatac catactcgtt tagactgtta gcgcagagtt
    13441 aactaggcaa tcgctgcttt cgtaagaaag gagaggaaag tccggactcc atagggcagg
    13501 gtgttggcta acagccatcc acggtgacgt gcggaatagg gccacagaga cgagcgtatt
    13561 gagttacggt gaaacgcggc aacctccact cggagcaatc ccaagtaagc aaatgttgac
    13621 atggcccgtc gagtttgcgg gtagggagct tgagcctgta ggtaactgca ggcctagagg
    13681 aatgattgcc ccttaatgca aattagggcg acagaatccg gcttatcggt taactctgca
    13741 ctacttcaaa gctataggta atctcagccg ttttgccaag cattgcagtt gctgagcagt
    13801 atttatcgtg agagagtgaa atggcacgct caactttgct gggatctaag ttcgagcctt
    13861 tcaccaaaaa atgcagatta attttggtga ataccttagg atcggattca gcccgctcgg
    13921 cctgtaccga aacttcacaa gcgctcaccg attgacggga gcgctgtagg attaaaacga
    13981 catcaaacgc tgtacagcct ccggcacctg ctagtagtaa ctccattgga cggggtgcaa
    14041 gattattccc tccagcctca ggggcgccgt ccatattgag gatatggccc gttcctgtct
    14101 gtgccataaa tgccatccca gcttcaggca accagtttat tttgcattcc atcatataaa
    14161 aatcctcaaa aaatattatt tctatttaaa tcaatgtgtt agattaaaaa atggccctgt
    14221 aagtcttata taaaagatag ggtaaaccca aattttttct tgcattgcac aagttttaat
    14281 cttaaaatga ttacattgat gtcaatcttg atttacaaaa gttgtttttt tactatcaag
    14341 tttgcattct actgatgtct cctccaccca aacattggtg gatttcaacc cgggattgat
    14401 tcccgggttt tttttgatat acaattcaat gctttactga attaccgatt cggtcagcgg
    14461 taatttgttg taccaattga gggattagat ctgcgagcct gcaatcataa gcggagccaa
    14521 agtgcggttt ctaaattgga gtgtgttgac caaacaattt gaaagtaatc atgaaaacct
    14581 tttccgcaaa accgcatgag gtaaagcgcg agtggttcgt gattgacgct acggacaaag
    14641 tcctcggtcg tgtcgccagt gaagtggcac tccgtctacg cggcaagcac aaacccgaat
    14701 ttaccccaca cgttgatact ggcgatttca ttgtcgtcat caattcatcc aagctccgcg
    14761 taacgggcac caagggattg aataagattt attaccgtca tagtggttat ccaggcggca
    14821 tcagctcaac aaattttgac aagatgcaag cccgttttcc aacacgtgct ttagagaaag
    14881 cagtgaaggg gatgttgcca aaaggcccac tgggctatgc catgatcaaa aagctgaagg
    14941 tttatggcga tgcagatcat ccacatacgg ctcaacagcc aaaaccttta gagatctaag
    15001 gaagcgatat gtacggaaat tggaactatg gaacaggccg tcgcaagagt tcagttgcac
    15061 gtgtctttat taaatccggt aaaggtgaaa ttactgtcaa tggtaagcca attgatgctt
    15121 actttgcccg tgagacctcc cgcatgatcg cacgtcagcc actcgctttg acaagtaatc
    15181 tcactacctt tgatattcaa gttaacgtta gtggtggcgg tgaaactggt caagctggtg
    15241 cagttcgtca tggcgtaaca cgagcattga ttgactatga tgcagcctta aagccaaccc
    15301 tttcaaaggc tggcttggta actcgtgacg cccgtgaagt tgaacgtaag aaagttggct
    15361 tacacggtgc tcgtcgtcgt aaacaattta gtaagcgtta agcaaaccaa attgcatcct
    15421 ggggccgctt acgcggccct tattttttat acaatcaatc tatatcgccg tttataaatg
    15481 gagaagagca tgattaaggt cggcattgtt ggaggtacag gctataccgg tgttgagcta
    15541 ctccgtcttt tggctcagca ccctaaggtg caattacatt caattacctc gcgcacagaa
    15601 gcgggcacgc cggtcgcaga gatgttttct tcattacgtg gtcgggttca tttgcaattt
    15661 acaacgccgg ataaagctaa tctacacgaa tgtgatgcag ttttttttgc tacaccacat
    15721 ggcgtggcaa tggcgcaagc taaagaatta ctagctgcaa atgtaaagat actggatttg
    15781 gctgcagatt ttcgtttgca agataccgct gtctttgaga aatggtacgg tatgccacat
    15841 gcctgccccg agatcttgaa agaagcggtg tatggtttac cagaaataaa tcgggaagcc
    15901 attaaaaagg cacgtctggt gggtttagca ggttgttatc caacttctgt gcaattaggt
    15961 tttgcaccac tgctctcatc caaaaatggt ttgaatcatc cattggttga atctcaaaat
    16021 ttgatcgcag actgtaaatc aggaacatct ggcgcaggac gtaaggccga gatccccaca
    16081 ttactttcag aggctagtga taacttcaag gcctatggcg ttaaagggca ccgtcattta
    16141 ccagaaatta tgcaaggcct aaaggcgatc gccggtcatg acgaccttgg attgaccttc
    16201 gtgccccacc taacaccaat gattcggggg atccactcga ccctgtatgc acggattctg
    16261 ccggaggggc gttcaataga tttccaggcc ctttatgagc agttctatgc caatgagcca
    16321 tttatagatg ttttaccccc tggcagccat ccagaaactc gctccgtgcg gggaagtaat
    16381 ggtatgaaaa tagcgattca tcgcccagga ggcggagata ccctagtaat cctagtggta
    16441 gaggacaatc tcgtaaaagg ggcttcaggg cagggggtcc agtgcatgaa tctgatgttt
    16501 ggttttcctg agaacatggg tttggagcag attgccctct tgccctagat gcttctatgc
    16561 accctgtgca aacaggggaa ttaaagccta aaatggatct acaacagaaa ttttgacagg
    16621 agtagcaaat gagccaaacc aatgcagcag tcgatcaaag tacagctctt gctgagccac
    16681 caaccccttt aatatttacc gatagtgcag cagccaaagt tgctgatctg attgccgaag
    16741 agggtaatac agatctgaag ttgcgagtct ttgtgcaagg cggaggctgt tctggatttc
    16801 agtatggctt tacttttgat gaagcggtca atgaagatga cactactttt gaaaagaatg
    16861 gtgttacttt gttagtggac tccatgagct ttcagtattt agtaggtgct gagattgatt
    16921 acaaagaaga tatcaatggc tcacaatttg tgattaagaa tcccaacgca accacaacct
    16981 gcggttgcgg atcttctttc tctgcttaag tagctgggta atacgcacca agcacgcgta
    17041 aaccttttgc acccgtaacg gctggcatat ttgctggccg ttttctttgg tgcgcccaag
    17101 ctagccaggc aaaagccaaa ccctctacta gttgagggtc aacaccatgc tgcgcagaag
    17161 actcaatcag tagcccagga agttgttgat gggcataaat tttgagaagt tcgattaagg
    17221 taatattttt actgccaccg ccacacacaa ttaggcgttg agtatctttg gcatgagctt
    17281 gcaaactatt tagagtgctc tgaatcgtta attgcaataa ggtagcctga atatcttctg
    17341 atgaataaga ggcatgctgg aggtgactta gaatccaatt tagattgaag taatcccgtc
    17401 cggtgctttt tggaggaagt tttgagaaaa aggcatcgct catcattctg agcaaaagcg
    17461 cgtcgtcagg acggccagag cttgcccatt gaccatcctg atcaaaatcc ttgcctcgct
    17521 ctctccgaat ccaggcatct agaagaatat tccccgggcc gcaatcaaag ccactaaccc
    17581 cgccatcctt agataagagt gtaaggttgg ctattccacc caaattaaga accgcacgat
    17641 tttccttggg tgaaaaaaat tgttcttgat gaaataccgg aactaaggga gcgccttgac
    17701 cccctgctgc catatcccgt aaacgaaaat tagcaattac atcaatacca gttagttctg
    17761 cgagaagggc ggggttaagg ctttggtggg tatagctctg taagacctcg ctatgcggtt
    17821 ggtgtctgag ggtttgtccg tgagcgccaa tagcacaaat atggttagcc tcaataccct
    17881 gaattcgtaa gagttttgca gaggcttgtg catacgcagc tgccaagcta tttgcagcca
    17941 aatgctcacg atggagctca ttggaagttt cctgctgaag ggcaaaaaat tcttgtttta
    18001 gagctaaatc aaatgggagg ctaattgctt gcaaaagctt tgttttacct gaggtattta
    18061 tttcagccaa gaccgcatcg ataccatcta agctggtacc agacattagt ccgatataca
    18121 gattacttga cagggatgaa ttagcagaat tcaagggtgg gtgctcaggt tctcgacaat
    18181 gcgacaatga cactttaaca atatttagac ccttaattta accgaatcta gaacatagta
    18241 tgacgtctaa accttatccg ataaccccat ctgttttgca tgccatggaa gtaactaaac
    18301 gtggctgtga ggagctattg gtcgaaacgg attggttaca aaaattagcc cgtagtgaag
    18361 ccaccagcaa gcccctgcgt atcaagctgg gactagatcc tacagcgcca gatattcatt
    18421 tgggacatac ggttgtttta aataagctgc gccagcttca agatcttggt cacaccgtga
    18481 tcttcttaat cggcgacttt accagcatga tcggtgaccc ctctggccga aataatacta
    18541 gaccccccct cactgccgaa gagattgctg taaacgctaa aacctattat cagcaagcta
    18601 gcgctatttt ggatcctagt aagaccgaga ttcggtacaa cagtgagtgg tgcgatccgc
    18661 taggtgcaag aggcatgatt cagctagcag caaaatatac ggttgcccgt atgttagagc
    18721 gcgatgactt tactaaacgc tatcgcggtg gcatccctat ctctgtccat gagttcttat
    18781 acccactcat gcagggctat gactcggttg ccttacatag tgatttagag cttggtggaa
    18841 ccgatcaaaa atttaactta ctagttggtc gtgagttaca gaaagaatat ggccaagagc
    18901 ctcaatgtat tttgacaatg ccactcttgg taggtttaga tggtgtcgag aagatgagta
    18961 agtccaaggg caactacatt ggtataaatg agcccgccaa tgagatgttt ggaaagatca
    19021 tgagcatctc cgatgatttg atgtggaatt atttcacctt actctcgttt aggccattag
    19081 cagaaattga tctaatgaag caagaagtgg cggcaggccg caatcctaga gactgcaagg
    19141 tccttttggg gcaagagatt gtggcacgct ttcatacaga tggcgcagca gagaaagccc
    19201 tagaagactt taatcatcga gccaagggcg gcatccccga cgatattcct gagcttcaac
    19261 taagtggcgc cccacttggc attgctgctt tattaaaggc tgctaattta gttccctcaa
    19321 gctcggaggc aaatcgcaat atcgatcaaa gtggagtgcg agttgatggc acagtaatta
    19381 ctgacaagac cctaaagctc aatgctggta cttatgtagt gcaagtcggt aagcgccgct
    19441 ttgcaaaagt aaccctgact gactaaacgg cacagtaatt aaaacagtat taatccagca
    19501 aatctagatt agcggagtta ctgggtggtt ttaggccaaa gtgttcatag ctttggcggg
    19561 ttgcaattcg accgcgtgag gtccgttgta aatagccctg ttggatcaag aagggctcaa
    19621 gcacatcttc aatcgtatcg cgttcttcac caatggcagc ggctaaatta tcaataccaa
    19681 caggaccacc attgaactta tgaaggatcg cttctagtaa tttacggtcc atgatgtcaa
    19741 agcccatcgg atcaacatca agcatagcca gcgcagcatc tgccatttct ttagtgatca
    19801 tgccactacc tttgacttct gcataatctc taacccgacg taaaagacga ttagcgattc
    19861 tgggggtacc gcgagcacgc ttcgcaatct caacagagcc ttgtggatca atttgcgcat
    19921 tgagtaactt ggcagatcgc tcaataattt gcgttagttc tggggtggag taaaactcaa
    19981 ggcgggcgac aataccaaaa cgatcacgta gaggattggt gagcatgcca gcccgtgttg
    20041 tggcgccaat tagggtaaat ggtttgagat ctaatttcac actacgtgca gcaggaccct
    20101 caccaatcat gatatcgagg gagtaatctt cgagagcggg atataaaatt tcttcaacca
    20161 cgggtgataa acgatgaatc tcatcaatga agaggacatc attctcttcg aggttggtta
    20221 agagggcggc aagatcacca gggcgatcta agaccgggcc gctcgtttgg cgaagattga
    20281 caccaagttc tttagcaata atatgagcca aggtcgtttt accaagacct ggcggaccaa
    20341 aaagcagaac gtggtctagc gcctctttac gatttcgggt ggcattaata aaaatctcga
    20401 gctgggcgcg ggctttgctt tggcctacgt actcatcgag ctgcttaggt ctcagggcac
    20461 gctctataac cgcttctgca ttaccagcac ctggactaac aatacgatca ttgccgtcat
    20521 cgatatcaag atcatctgta tgaattgcca tatgtgatca gcccttagaa agtgatttaa
    20581 gtgctagctt aataccttcg gacacattca catcagccgg aatttgacga actgcaagat
    20641 gagcttcttt atcagaatat ccaagcgcca gtaatgcttg agtaatttca gaggcgacat
    20701 ctggtttgct agatttgttt tggccatgac ctaagtcggg ggcgagcttc ccttttagct
    20761 caagcaggag gcgctcagct gttttcttgc caatcccagg gacccgagta agcgccccag
    20821 gctcctgaat ggtaatcgct tggaccagtt cgttaacgct catcccagat aagactgcta
    20881 aagcagttcg cgcacctaca ccactaattt taattagaga tcgaaaggca tcacgttcgg
    20941 catccgttgc aaagccaaat aattgttgta gatcctcgcg tacatgaaaa tgaatcagca
    21001 atgtgagctt ttgtccaatc ggggggagtt gatacagtgt gctcattggc acatcgacct
    21061 cgtaaccaag cccattgcaa tcaatgagta atttgggggc gttgatgcta attaaggtgc
    21121 cttgaatacg tccaatcatg cctctatctt aacgcttcgc atgactgtta tgtacttggt
    21181 gatgatgggc cgcacaaatg gcaaccccta gggcatccgc tgcatctgga gagggactct
    21241 ttttgagtct gagtagacgt ttgaccattt cttgcatttg aagtttactt gctcgaccag
    21301 tacctacaat ggcctgctta acacctcgtg cactgtattc tgaaacgggt aaacccgttg
    21361 agaccaaggc tgcaatcacg gcaccccgag cttgtcctag catcaaggtc gagcgtgggt
    21421 tgacattaag gaagatttct tcaatcgcag ctgcatcggg ttgataggtc agcaagacct
    21481 cttgaacacc gctgtataaa ataccaaggc gttttggcaa accttgctct acctcaccac
    21541 tttgaataat tccggagctt atatagcgta agtgctgacc ttcaag
//
LOCUS       DDPQ01000095            5672 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_27596,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000095 DDPQ01000000
VERSION     DDPQ01000095.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_27596"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1932..2008
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2454..2537
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5264..5358
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1459 a         1311 c         1185 g         1461 t
ORIGIN      
        1 tcggcggggt tttttattag aattagacag cccatgaact ccttcgcagc gaactctcct
       61 ttaaaacgct ttgtgcgtta ttttctctat ttactgggtg ttcttttatt actgattgca
      121 ggcttgatag cgacttattt agtttctgca aaatctagct tttcaggaat cgtaacgagt
      181 gcaacactga atcagccagt acaaattcag tttgatgaaa atgatatccc tcacattaaa
      241 gccaagtctc aaagtgatgc gttttatgcc ttgggattct tgcacgctac cgaacgatcc
      301 tggcaactcg agcttagtcg acgcctagcc tcaggtcgtc tctctgaaat cctcggagag
      361 cgaacagtat ctttagatcg tttcctaaga acgctgggta ttaaatatgc cgctgagaaa
      421 caatatgaac gctatcccac agagattaag caactcatta aagcgtatgc cgatggagtg
      481 aatgccggca atcaatcctt gggttgggcc ttacccattg agtacttttt aaccggctcc
      541 caaccgggcc attggtcgcc cacggatagc gttgcctggt ctttgatgat ggctcttgat
      601 ttaggggata actggcaaaa agagttattt cggcttgagt tatcgcaata cctcaccact
      661 gcacaaattt gggatgtgat gccgccctat ccgggtaatg ctccagtgag caatcttgac
      721 tttgcaaaac tctataaaga gcttggggta tttaaacagt cttccaagtc atcagaaaag
      781 caatctcaga acaaacaaca tgatccatac cttctgcaaa gtaccgattt gcagccctgg
      841 ataagcgata acaaagaagg ggtgggatca aataattggg tcttagccgg caagaaaacg
      901 gtttctggta aaccactatt agcaaatgat cctcatttgg gtctaagcgc accatccacg
      961 tggtattttg ctcacctcga agcgccaggt ctgaatgtaa tcggcgccac actgcctggt
     1021 attccggcgg ttgtattagg cagaaccacc aaaattgctt ggggcttcac gaatactggt
     1081 cccgatgttc aagaccttta tctagaaaag attgatccca ataatcctaa tcagtacatc
     1141 ggccctgatg gtcctctctc tttcttggtt cgcgaggaga tcatccaaat taaggataag
     1201 ccgccattac gttttgtggt caaacaaagt cagcatggtc ctattatttc agatagtcac
     1261 gcacgctctc agaagatcat tgatagcaaa cgtttcgctc tggccctacg ctggaccgct
     1321 cttgatactg agaaccagac cttgctcggc gggatcatga tgaatcgtgc agactcgatg
     1381 gagtctttta aatcagccct acgatatcac tacgcaccca tgcaaacggt cgctattgcc
     1441 gacacgcagg ggaacattgc acttcaagta gccggaacga ctccgcgtcg acagcctggg
     1501 caaggtttgt atggggtagc cccagcacta ggctgggaac gttcgtttga ttgggttagc
     1561 tacctgccat tcgaacaact tcctagcgcc atcaatccag attctggatg gctggcaagc
     1621 gctaatcaac agattatttc taataccaac ccaaatccat taacaggcga ctgggatcta
     1681 ccatttcggt atgaccgaat taaatccatg cttgaagcta aagatcaaca cgatcgagct
     1741 tctatgcaaa ccatgcaagg cgacaccctt tctcttgcat ccgttccatt aatggatctt
     1801 tttaaacagg ccaagaccaa tcactctctt caagcagccg cacaatcatt catacagtct
     1861 tttgatggca atatgacaaa agattccata ggtgcgctga tctttaatgc ctgggcagat
     1921 caacttaccc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta gaaaactgcc aagatgctgc gaatgtagcg
     2041 cttgataaag cgttggcgga tctaaaagaa cgccatggca actcaccgag tgactggcaa
     2101 tgggggcaag cccatcaagc gctctcggag caccgtccct ttaaaggttt acccatcctc
     2161 tcgaattttt ttaatgtacg tgtaccgatt cctggggatg catttacggt caatgttggt
     2221 aggccggcct taaacaaccc ggcggcccct taccagtcca atcatgcagc tagcttacgc
     2281 gccatctatg acctcaatga tctagaacag tctgtcttta tgtttccgac agggcaatcg
     2341 ggctgggttc aaagtggccg ttatcgtcat ttaagtaaag cgtggtcgga taatgcattt
     2401 ttaccgctaa ccatgaatcc caatcaagtc agtcgtgaga ttttgataaa aagnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnagt ctctttctag cagtttcaac gactacatat gcagcctggg
     2581 atgaacttgg tagcaatgaa ttcatgaccg ttatgattga taaagccagt attaaaaaga
     2641 cgggagataa agttcaggtc cgctcgatgt tggatctcaa aaaaccgggg gttgagccca
     2701 agaccaaagc gcctgtgaac tccattattg gtttaaatga ataccattgc ggtcaggtgc
     2761 aatatcgccc tctcgaagtg aaatttatgg ctggcaagaa aggaactggc aaggtgatcg
     2821 atgagatcaa aactccagat agttcgtttg aggccgtggt gagtggagac tgggccgcag
     2881 gagtctataa ctttgcgtgc cgtccttaaa gatcttttct catcagaagc tcatagggcc
     2941 ttggtccgcc cagcccttgt tttattagca ctctcaagca gcctactctt acagtcttgt
     3001 gctgcccctt ttatggcggc tgccagtagc gtaggaacgg ctgctggttc tgctgcggtc
     3061 gccagtcctg tgaccgcggt cagcgtaacc tctacagtca ctacaggaag atctcctctg
     3121 gagcacgcca catctgcggc taccaagaaa gattgcagtt tttttaatgt ctttagcgct
     3181 aagccaattt gcgaagagat cgtgatccct agcgtcaaag atcaaagtga gatgattatt
     3241 ggtgaggcag atcgtagtgg atccgtcatc gaaattattc aggtacgacc gagtaaatag
     3301 tggggtgaac gacgggactc gaacccgcga caaccagaat cacaatctgg gactctacca
     3361 actgagctac gtccaccatt gctacataag tattataact tcccgagagc cttgatgaac
     3421 taggctattt gagcgtttaa atcaaaggct gcccagtctc ctaactcaag gctcgcctca
     3481 aaaaagaact cctcaatcgc ctcttttgtc ttcactttgg ctaatgcccc atgatccgat
     3541 aaacgctgac gccagcgtct agctcccgcc cttccatgcg caagacccat aatatggcga
     3601 gtaattgctc caagatagaa tggcttttgc cgttcctggc aatcatcaaa ccattgcttg
     3661 gtttgctgaa ccaatccaat ttggatacga tgccattcag tttcactaaa aagatagcca
     3721 gcagctgagc cctcggactg caacagatca tcccaaccca ataacatcgc cgggaagtga
     3781 taggcagatc tgccaaccat aaagccatca aagtcttgcc agtgattagc aatctgctca
     3841 ttgctctcca ggccgccatt tagcaatact ttgagatggg gataggattt ttgggcatcg
     3901 atccgtaatt gcttagcaac ttcatagtgc aaagccggct tggtgcgatt ttcctttggt
     3961 gacaatcctt ttaatacagc attgcgcgca tgaatggtca cctgagaggc gcccgcatct
     4021 gcaacagcca acatgaaatc caagacaaat tgataatctt cttttgactg agaggcatcc
     4081 atttgatcca agccaagccg atgctttact gatataggtc ggtcatatac cgatcgcatc
     4141 gctcggacac actcagctac caatgcaggc tctgccatta agcaggctcc aaatgcgccc
     4201 ttttggacac gttctgaggg acagccgcaa ttaagatcga tttcgtcgta gttccactgc
     4261 tgggcaagct ctgcacaacg agccaaatca gagggttcgc tacctcccaa ttgcaatacc
     4321 actgggtgtt ctatggtgcc gtaatcgaga tggcgggctt ggtccccatg taataaagct
     4381 ccactagtga ccatctctgt atagagaacg gcctcctttg taaggaggcg atgaaacgat
     4441 cggcaatgac gatcggtcca ctccatcatt ggagcaacgg cgaggcgttt cttattcaaa
     4501 gacctattcc cacggattaa tcaatttaac ttttgtagag tcaaagtcaa cagtatttcg
     4561 ggtaactaaa gttaggcgat gttcaattgc agtggcagca atcattgcgt ctctatctga
     4621 agatggatta gggatatgaa gggatgcgca acgaattgca attggtgtat tgattggcaa
     4681 aattctatca acaaaagcag ggagaacgaa ttgcattagc cattttctta aaacctgtga
     4741 ttgtttttta tctcgtctct caagtcgtag aattcccaat tcaatttcta aaatagtgat
     4801 tacagataga aacatagtcg atggcatttt tgtttctgcc cattctttga ctcttggatt
     4861 tattctgctc tcagtagttt ttctgaactc tgaaatgaca ttggtatcta gtaaaaacat
     4921 tagcttaagt ctattggctt agtaaagttt tctattttcg caggctcaaa tttaatatcg
     4981 ccagcagctt tcatgcccac aagatcgaga atgttttttt gatggccaat gactctttgg
     5041 taatcctcgt acagcatcaa aacatgtgag ggcctaccgc gatctgtaat aaaaaccggt
     5101 ccagacctac aggccttttt tgcaccgctt gtatcctgat taaattctct actagacagg
     5161 gtttttggca cgccgttttt cctcttaaac tataatgtag taatgttact acattatagt
     5221 agcatcgatc caaagcccta aagttcagac ttgaggacta aacnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnac ctcaacaagc tcgtcgtctg aaatgaactc aacggcatat
     5401 tccagagtta attgcacagg cgtaaccaag cggaccgctt catctgttcc agaagcgcgc
     5461 acgttagtta actgcttacc cttaatggga ttaacaacga gatcattatc tcgactatga
     5521 ataccaataa tcatgccctc gtaaagcggg tctcccggtg agacaaacat tcgtccacgg
     5581 tcttgtagct tccacagggc ataagctacc gcctcaccat catcttgact aatcagtacg
     5641 ccgttatggc gttcgcccaa gataccctcc tt
//
LOCUS       DDPQ01000096            2973 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_24209,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000096 DDPQ01000000
VERSION     DDPQ01000096.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_24209"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    586..655
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          738 a          696 c          655 g          812 t
ORIGIN      
        1 gcttgctcaa tcgcgttttt taactcgacg acattgggtt cacaaacagc atcaaatgag
       61 ggcatttcgt tctcctgctt tccaaggttt tggtaataat tgaattttag gtaatttggc
      121 tttgttttat ccactgtttg caaaacccga ataagattgt ggcatgaact tgcccagcaa
      181 tctactccct gcgtattcct taagccagcg caataccttg ggttttgagg ttgaggccga
      241 gtttgcaatc cacattaccg aagctaagca aatcgccgag acgcttgcgt atgccaaaga
      301 tcacaatatg ccctatcaag tcttgggtgg tggaagtaat gtggtgcttc ctagcgccct
      361 acctggtctc acccttctca tggatatcgc tggaatcgaa atagtctctg aggatgctaa
      421 tggggtgagc ctaaaggtgg gcgcaggtgt ctcgtggcat tcgctggtga tttggacgct
      481 tgagcactcc taccccggtc ttgagaactt agccctcatt cctggcacag tgggagctgc
      541 ccccattcaa aatattggag catatggagt tgagattgaa aactannnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaca
      661 atggcaaccc agaattagct atgcagactt acaacacttt tttgctggga aggagaatga
      721 ctccattact ccgctggcaa tatttgaggc tgtttgcgag atccgcactc aaaagctacc
      781 agaccccaaa cagattggca atgtgggcag cttctttcaa aatccaattg tgnnatgctt
      841 gccgaactca agcaaaggca ccctgcaatc gtcgcctatc ctgaggatac aaatcacagc
      901 aaattagcag cagggtggct gattgatcag tgcggattta aaggtgtgca agtaggctcc
      961 gttggagtac atggcaaaca ggctttggtt cttacccatc ggggtaatgg taattccaca
     1021 gaactactga tgcttgccaa aaccatacaa gagactgtgc gtgagcgtta tggaattgat
     1081 ttggttattg aaccggttat ttataagtag ttttttgaaa acttatgctg cgattaattc
     1141 gcaaggtgta attttcttca atactttttt caaactctca cgcgcaattg cagtgtcata
     1201 atttgcctga ccgcgtaacc accatccatc actcaaacca aaatacttac aaagtcgtaa
     1261 atcagtatcg gccgtaatgg cacgtttacc cgcaacgata tcgccaatcc gttgaggagg
     1321 gactccaata tccttagcaa ggcgatactt cgttaatcct aggggtttta aaaattcctc
     1381 ttccaacatc tcaccaggag aaactggggc tattgctctc atactgattc ctttctagtg
     1441 gtaatccaca atctcaacat cttttggtcc gtcggcagtc caaataaaac atactcgcca
     1501 ttgctgatta attcgaatac taaattggcc tttacgttcc cctcttaaag cctccaaacg
     1561 attacctggc ggtacccgga ggtcttcaag tatcgatgac caatccaatt gttctagctt
     1621 tcgcaaagct gggcgctcta ttgagagcca gcgtcgtaca cgatgaccat gaaacaattt
     1681 ttccgtgtcg ggacacttaa aacttttgat ggacataatg aatattaacg tataacgtta
     1741 atagttgtca accatcattt agtcgatgaa gttaaaaacc gagtctttac ttcgccctcc
     1801 gccaaacgaa ccaagtattc ctggtcggct ttgatgtcat ttggttttct ggcggccttc
     1861 ttgtgttgat ccaagataac tgcatacccc cgctctaagg ttctctgtgg attaagagtt
     1921 tcgagctgtc tcagccagtg cgcttgattg tgtttgagat tattgaaata aacatgggca
     1981 ctttgcacga ggcgctgttg ccactgaccg atttgctcac gcatacgctc agggttaggc
     2041 agtgcatgcc ctaagcgcag actgatttga tctagggttt gtgcctctcg ctcaatacgt
     2101 tgctccagct tttgtcttaa ctgctcctga tatgtacgta actcttgcaa tagttgctca
     2161 cgcttaggag ttgcaagctc tgctgcacca gtcggtgttg gtgctcttag atcagaaaca
     2221 aaatcagcga tggtgaaatc ggtttcatgg ccaaccccac taatcactgg gatagaagag
     2281 cttgcgatgg catgggctag cgcttcatcg ttaaaggccc agagatcctc aatcgatccg
     2341 ccaccacgaa cgagtaatat gacctcgccc aatcgatcct ggttggcttg cttgagtgca
     2401 tgaatgattc cggcgggggc gtcaggtcct tgcactaggg ttggataaat gaagatgggg
     2461 atatggggcg ctctcctagc gagtgttgtc aacacatctt ttaatgctgc tgcttgcgct
     2521 gaagtaatga tcgccaacga tttaggatga ctcggtatgg gtcgctttcg attctcatca
     2581 aagagccctg ctttcgctaa tttctcttta agctttaaga aggcttcgta gagccctcct
     2641 aagcccgcct ttcgtaaaga atggatggtg agctgtacgt ccccccttgg aacatacatg
     2701 ctgaccgatg cagcgacctc cacctgatca ccagattggg gcataaatcc aagctgggcg
     2761 ttcttacccc gaaacatcac acagcgaatc tgcccttctt cgtccttgag tgagaaatac
     2821 caatgcccac tgtcataagc cttgaagtta gaaatctccc cgctaaccaa gacagcatca
     2881 aaacgctcgg ttaaagactc tgcaatggcg cggtttaaat cgccaacact cagcaggggg
     2941 cttgatgtat ccatggttga ccttgtttat ttc
//
LOCUS       DDPQ01000097            5807 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_38729,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000097 DDPQ01000000
VERSION     DDPQ01000097.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_38729"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    4075..4129
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1550 a         1251 c         1325 g         1626 t
ORIGIN      
        1 gacttacatt aatcaacgct atccgggttt taatgccccc tacagcggcg ccaatagttt
       61 gtctaaaaat ccatccatga gttacacctg gacaggcacc ctcttcttgg gtgctcgctt
      121 agcacccaat acggatattt actttaatcc cgaggtgatc tcaggcgtgc ccttttcagg
      181 tcttgtgggc ttgggaggat tgagtaatgg cgaagccaac aaagcagccg gctcgcaagc
      241 caagttttat tcggctcggg cgtttttacg atcaactatt aatcaagatg gcgagaaaat
      301 ttatctcgaa gatgaggcta atcaaattgc gcaaaccgta agcagtaatc gctttgttgt
      361 cactgctggt caattttcta cgctcgatat ttttgacgat agtcgctacg ccaaagatcc
      421 gagaacgcaa tttatgaact ggggtaattt aacttacctt gcgtatgact atgctgcgga
      481 tagtagaggc tatggctggg gtttagctgg tgaatggtac ttggaaaact gggtctttcg
      541 agcctcgcga atgacaacgc caagagaccc aaatggttta ccagttgact ggaacatcat
      601 gaaacactat ggtgatcaga ttgaaataga gcgaggtcat tcaatcagta accgccccgg
      661 aaaagttagc gtactagcct accgcaataa gatggttcta gctcgatttg ctgatgctac
      721 gaactacgtt attcaaaaca atgctcaagg tactcaagca attaataacg tacgtaccaa
      781 tgaacaattt aaaacgggtt ttggtatcaa tgctgaacaa gcaatcacta aagatgttgg
      841 tgtatacatg cgagtattta agtctgacgg acaaacagaa acaatggcgt ttgctgaagc
      901 tgaattggac ctgatgtagg cggggccata gattcttgta attgatgacc tgaccaacgc
      961 ccctttaccg agtaggctcg gagttttact gtgaataatt cgcttgcatg acttcggtac
     1021 caatctaata atggtgactc aaccataaat cgattggctt gctcaagccc ttcaaaaaga
     1081 caaaataggc cttgatgggt tccgctcggg ctaattaaga atccaccatt tttacctgat
     1141 cccatatgct tttgcagaat aagaccttgt ggtcggtata ggctaaatcg gttaaacatc
     1201 agacgggcaa taccagaata tttagcttgc ggtttgatat tgatcaaggc catcaccgct
     1261 acctgattag acatcttatt cctcggtgtc gctatctttg ctttggtcgc tcagatcgta
     1321 tttacgtaat ttaatatata aactttgccg agaaagcccc agcatctcag atgcggatac
     1381 tcgattccca cgcgtgaggt ctagagcggc cttgatacag agttgctcaa ttaaatcggt
     1441 ggtctcatta ataatttctt taagaggcag gcgaccaacg agttgggtga gttcttcact
     1501 cgaacgagta atggactctt caggctgtat cttagagcgt atgcgtgagc ctacttctcg
     1561 aatagtaaat ccaagacagg catgcgggta ggggacagat actgcagaaa tctcaacggg
     1621 atgcaaagtg ccaaaggagt cacgaattga ggtagcgaat aatttaattg atcctttttc
     1681 atggagattg gacagcatga cacgcaaatc aacactagag cgctctagcc aacggtctag
     1741 gggctcattc agaatttgct ccagtttgtc tgacataatt aagcgtaaga aagcttgatt
     1801 agccgttaag atttttcctg ctgagttggt cacaataaaa ccatcgggcg cattttcaag
     1861 ggcctgtatg actagtgatt gcgcatttga atcatggcga tcaaccaact caccagagcg
     1921 tttcagggta accagaatca gcgattgatt ttcattacga aatggtgctg ctgataggta
     1981 gacctcttga ccactcaaaa tgctcgtatt taactccgct actgtgctgg tataggctac
     2041 cttactaagt agagattgaa cctgaattcg gtctcccttt gtgaggtagt ccatcaataa
     2101 gcgaccactt aattttttta cagagtcacc gagtaaatca gcagcacgtg gatttgcttc
     2161 aatgactttg aacgtattgg catctaaaac aatcactgcc tcagatgcca tttcaaatag
     2221 aacgcgatag cgtgtctcta gttggcgtaa gcgggtatag tcgcgttcaa tggcttgttg
     2281 agcatcaacc aagcgctgtt gtaataagga aatttctcgg agatcgcgcg cgagtgcgat
     2341 gatctgtcca gtgctaccaa cctttaaggt cgcacacagc agcggtaaat cggcagagcc
     2401 tggagaaggt acattaattt gtctaaaacg agacaaactc gtctcattcg catcttggag
     2461 aagagcctgt atttttggac gactttcgct agtaactaca tctaaccaat ttttaccaac
     2521 ccaactttcc aagcttgggc ttcgtaaatc ggggttacca aaggcaatac tttgaatgac
     2581 gccatcgcga tttaatgtta acgagatatc tgcagatgct tgaacaatat gagcaacctc
     2641 atcaggattg agttgcccta ggacatgagc tgggcttgtt atgctgttca ctatctactc
     2701 gtgtatcgat ttaattaaac gcgtgatttt aagatatttt ctgcggctat tactgcgtct
     2761 gcagcactta aggtgcagat atcggcaaca catgaatcca ttagctcagg ttttgcatta
     2821 aacaatgcgc ctcccgccat aatcccaatt tgtgaattga gagagactcg ctttatcttt
     2881 ggaatgagtg cctgtaattt ctcccattga agctcatacg aaattgaaat accaactaca
     2941 tcaaaaaatt ctttagaaca caattccaga acctgttctt cgctgggact tggggttgag
     3001 caaacatccc agccggcctg cgtaaagaac tcacttaaca taaaaacacc catcgtatgt
     3061 tgtgattggg gcattgcaaa gagtaaagcc ttaccagaag aactacgatg atgatgttgc
     3121 tcattccgaa aatcagggct tagttcttgc atgatctgct gaatccgtcc caatgcaaga
     3181 gtaactgttg taaagctatg ttcatcgagt tcccaatagc attgcattct tctggcggca
     3241 ggtgcaatga gatgtaggta gatttcattg agtgatcgac cctcaagact tagggacttg
     3301 atatagccca tacaaatatc gttagagcta ttaatcaata gctcagcaaa atatttcacc
     3361 tcgtctgcgg agataggctc gtcggcatgg gaggcaagcg cttgatttgc gttgctgtgt
     3421 tggattaata gtcgggggat gatttgagcc tcgatcgttt tatttagtag atgagcaagc
     3481 tcatgagggg tgggccctct cggggattcg ggctccaaac tcggcagtag agcacttaag
     3541 cggaactttt ttagccatcc tctactttcc tttgtgttag ttgaattacg tttctctgtt
     3601 tgtagattta aatgatccac ttttatatgt ccccatacag ttaattcatg ttgcaatgca
     3661 acatttatta catccaagac ctaaaattac cccaaaagca cgggttttag gccctgaata
     3721 gctcaaaaag gcggccccaa attgcctttt tcttcgatat ttataaggtt aacccctagt
     3781 tggcctaact gtcaaaataa tttgacactt ttaagggttt attcctagat ttcaaaacat
     3841 aaaaaagcgt aaacttaggt ttacatattt acataggagc caggctgcat aaggaattaa
     3901 gagcacatgg caagtcaatc acaatccctt tataccccag aagagcgact tcgacagaga
     3961 ccgctcgaag tggacattgg ttcaaggcat cctagcgcct gtgcaatttc tggtgttttt
     4021 aatcagtctt tatctcgtac tgcgatttct attcacgggt gagggtgagt ttgcnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttagtatgct
     4141 ggttttattc ttgcataccg catatttgtt tgctcttggt tttggttttc tagaaatacg
     4201 ccaactgatg gccttagcct tagcagctta cttggcctac gtgattaatg ccgcgcagtt
     4261 cttatggaag ttacgtcaag ctcgacttca agagccaaaa caatctccag gtggggtgac
     4321 agcatgaatc gaattattcc attgcaacct gctcagcagg atgcgactct gttgcaccat
     4381 taaatattct gaaagagaga gggcaacggg aagtcttttg tggtctaacc ggcattattt
     4441 ggcttcatcg gaaaattcag gatgcatttt tcttagttgt tggttcgcga acctgtgctc
     4501 acctcgtgca atctgcggca ggagtgatga tttttgcaga accgcgcttt gcaacagcca
     4561 ttattgatga tcatgattta gcaggactgg ccgatgccaa tgaagagctt gatcgggttg
     4621 tcaatcaact tcttacccgt cgaccagaca tcaagatgtt atttttggtg ggctcatgcc
     4681 catcggaagt gattaaatta gatctatcac gtgcagccga aagacttagt aaaacgtata
     4741 ccccgcgtgt tcgtattctt aattactcag gcagcggaat tgaaacaacc tttacgcaag
     4801 gcgaggatgc ttgtctggct gcgcttgcta aagagattcc acaagttagc gctgagcgcg
     4861 ctgcccaaca ggacgtagat cttctagtgg tcggttgttt ggccgatgtt gtagaagatc
     4921 agttctccag aatgtttaca gagttaggcc tttcgtctta tgctttcttt ccaccacggc
     4981 gctcgggcga tctgcccaag gtaggaccta atacgcgtta tctgttggtt caaccctttt
     5041 tgacggatac cgcacgtgtg cttgaggagt taggcgcttt gcggattcca gcaccattcc
     5101 cattgggtgt tgaaggcaca gaagcatggt tagccgccgc agcaaatacg ttcaatgtgg
     5161 acccagagcg ctttgagaaa gtagttgcgc caaaacggga gcgcgcgatg attgctttag
     5221 agcgtcaacg caagatactc aatggacgct cgatcttctt tttccccgat tctcaattag
     5281 aaattccgtt ggcacgcttc ttacaccgtg agcttggaat gcaattaaca gaagtgggaa
     5341 tgccttatat ccatcgtcag catcttgccg aggaaattac gctcttacca aaagacgttg
     5401 tcttatcaga gggccaggat gtagagaagc agcttgatcg ttgccgcgct gctatgccgg
     5461 atatggtggt ttgcggttta ggcttagcaa atcctttaga ggcagagggc atgaccacaa
     5521 agtggtcgat tgagttggtc tttacaccca tccaaggttt tgatcaggcc gctgatctag
     5581 ccgaactctt tgcacgtcct ttagtgcgta aaactaagtt ggcagcctag gagaacagca
     5641 atgaaattaa ctctatggac gtatgaagga cctccccaca tcggcgcaat gagaattgcc
     5701 acagcgatgg aagatgtgca ctatgtctta catgctccgc agggcgatac ctatgctgat
     5761 ttactgttca ccatgattga gcggatgaac aagcgtccac cagtgac
//
LOCUS       DDPQ01000098            3082 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_17285,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000098 DDPQ01000000
VERSION     DDPQ01000098.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_17285"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT          816 a          689 c          726 g          851 t
ORIGIN      
        1 atactgattt ggtagcgggt gggatcggaa acgcctgcct ctgtaaatcc agctttacga
       61 agttggcaag actcgcaaat accacacgcc tcaccctgac tattggcttg ataacaagat
      121 accgtctgac tgtagttaac gcccatccca gcgcccagct gaatgatttg cgccttactt
      181 aattgaataa ttggggcgtg gatacgaaag cgattttctg gatcgggcga ttcaatccca
      241 actttagtag ctagattcgc cattgattca aatgagcgaa catattcagg ccgacagtct
      301 ggatagccag agtaatcgac agcattggcc ccataaaaga tatctaaact tgaaattgac
      361 tcagcccacg ccagtgccag cgatagcatg atggtgttgc gcgcaggtac ataggtaatc
      421 ggaatacttg gcttcttgtc acctacgata gcttggtcct tacttgttgg aatatgcaag
      481 gaatgatccg tgagcgcaga gccaccaaac cgcggaagat ctaaatgaat aatctcatgt
      541 tgcaaaaccc cgatggtatg agcaatttgt ttagcagcct ctagttctga ggagtgtttt
      601 tgtccgtaat tgatggaaag ggcgtagcag cgataaccca gctgttttgc aagggtgagg
      661 attgtcgtgg aatcaagtcc accagaaaat aagacaacag ctggagcatt ttgggttcgt
      721 ggtttgctta gatgaaaagc agaggtagga gattgattca cggataaaac tacaatcgat
      781 tacaactcta gtttatttaa tgcttgccaa gataggcttc gcttcttttg cagcatcaga
      841 atctggatat ttagtaatta gatccgttaa caacttcttg gctatagcct ttttaccaga
      901 ctccagatta gcgtttgcca tagtaagaat ggctgctggc acgcgctgat gccctgggta
      961 gcgagtaatc agactctgta attgagtgat ggctgcgttg taatctttta ggacgtattt
     1021 ggtattgccg gaccaataga gcgctagagg aagataagga ctcgatggat atttgcgaat
     1081 aaaggtcgtg aaatcactgt cagctttttt accttggcct gcctgaaagg atttgagggc
     1141 gtcatcgtag gctgtctttt ctccagcctg aacaatgcca gtcaccccct ccacctcgac
     1201 agtttgtggt tctaggcgcg caaggcgcgc atcaaggtct tgataatagg tcttttgatt
     1261 gctacttaga tcatcacttt gtttttgtag ggtctcaatt tgaccgcgca attgtgcatt
     1321 ctcggatttg attttatcga tctgattttg gaggtccaag atagcagcct gtgatgcgct
     1381 aaccgattta cgtaactcga gaattgcttt acgtgcctca tcatctgaaa atagtgccca
     1441 tgtattttgt gaaagcagta atagcgctcc gaacacatac agggcaggtt taaatctaaa
     1501 aatcacgatg attactcgcc tttattactt ggtgagatag atgatatcgg cacggcggtt
     1561 ttctgcccaa gccgcctcgt tgttaccttc agcctttggc ttttctttac caagactaac
     1621 cgcttccatt tgaccatcag gcacaccgag agcagcaaac gctctccgaa ctgcatcgga
     1681 gcgacgttga cctagagcaa ggttgtactc agcagtaccg cgatcatccg tatttccttg
     1741 gatcatgatt ttttgttgag gattttgttt gaggaagact gctcccgctt gcagcatatt
     1801 ttgatactga ggtttgatgg tgaactcatc aaagtcaaaa taaatactgc gttggaatag
     1861 ggggctcttg ggatcattcc aaggctggga agcaaagtta ctactgtcgc taccgccggc
     1921 tttgacggta cttagatcat ccaacttaac acctgatgag caggcgctca agaagcttac
     1981 tgcactaaga ataccggcaa gggctaaacg acgctggggt gatgtggttt tcatatgctc
     2041 tccctcaagt aaatgcaact ttatttttat tcgatcaata caaatttaga agttgattcc
     2101 aaaccctatt atgccttctc atgatgacag gcccattcag aaactattga tccatgaatg
     2161 ggccccagga gggctggcgt acatccgatc ccggaatact taatatttgc ttaacgtagc
     2221 catcaacgga gactgcagcc aatacctttt taccaccgac ttgcgtggca taaagaatat
     2281 agcgaccatt tgcggcaaag ctaggagact catcaaagct ggtatcagtc atagcggtcg
     2341 aatcaccagt gagtaagttc attaagtaaa gcttgaatcc acccccaaca ccagcaatat
     2401 aagctaggtt tttaccgtcc ggagaaatgc gagccgaggt tgcatagggc tgcttaaagc
     2461 taattcgtct tgcgggctct cctcgctccc cctcagcact catgcgatag atttgtgggt
     2521 tgccaccgcg atcgctggtg aaataaatga acttaccgtc tggagaaaac tgtggctccg
     2581 tatcgatggt gctaccttgg gttaggcgtt gtaaattact gccgtcggca ttgacccgat
     2641 aaatttgggt attaccgtcc ttggataatg agatcgcaag cgattttcca tctggtgccc
     2701 aggagggggc gctgttgttg cctttggcat tagataaaac aatacgtctt ccagaggcaa
     2761 gctcatgtac atacacaatc ggtttacgat cctcaaatga cacgtatgca atcttttgac
     2821 catcgggcga ccacgccgca gaaataattg gctcactact attcagtgca ttgcggatat
     2881 tttgtccatc ggcatcggag atgactaagc gaaagcgctt gccctcttta attacataag
     2941 agaggcgagt tgagaacaca cctttctcgc caagaagctt aaagataatg tcatccgaga
     3001 tgcggtgggc tgctaaacga agattatctg cgctggcaga aacaattaaa ccattgagac
     3061 tttcgccttt gcgaatgtca ta
//
LOCUS       DDPQ01000099            4118 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_17673,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000099 DDPQ01000000
VERSION     DDPQ01000099.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_17673"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    639..698
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3589..3696
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3870..3994
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1014 a          961 c          941 g          909 t
ORIGIN      
        1 aatacggcat tcaatacccc agtgagtccg acggagatca acgatatgag ttcgcttgat
       61 aaaacagttg ctactgagtt tattgatcac catgcgcaat acacctttaa tcggcgcacc
      121 agcttaaatc gtttgcaggc tgtgcggatg accgctcaaa aagccaaagg ctggagtcgc
      181 agtgaaatcg atcatttgct aagcgatcag caagatgatt tggtacgcgt tggacagcaa
      241 tctcttaaga aacgatttga gggcttgacc gatgagttat taattgatga agtgtatgca
      301 gaccctgata tcgcacagac cttggtaacc tgcctcagca ttttgccccc atgccccagt
      361 atttttgccg tcgagcaggg ctcgatggtt tttgtagacc tcgatgcagt cactccaacc
      421 gaagatcagg tggttgcggt gggtggcatg ctggcatcga ttgctggcag cattgaagtg
      481 atggacaaag ggattcgtat tagttgtccc tcaagcctca ttcgcatgcc gattgtgaac
      541 ttcatccgtc atgatgagtg ttatgccatt cccgcagttc agtacatctc gtatgacacg
      601 atcgttcaat cggaaaaaaa tatctgggat cagttaggnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat tggtatgcaa accatgaaca
      721 ttcacttgga gttacctcat tccctatcaa aaccagtgtg gctcggtgga attgctctcg
      781 atggggctag ccgggcacat tactgggtgg cgattgaccg ctttacacga tgagtagcgt
      841 ttgctccctg cgactcagtt ttttgatcct cttgggactc gtctggctta gcccggttca
      901 tgctcaaaat caggcgcaag tgcagactcc agcgcagccc caaaaccaag ttaataccac
      961 cccaaatcag gctcttcaag cccctggcat cccgcttgag gtgcaaaaca atatccgccg
     1021 ttttattcaa aaaagcccaa ccgtcttagg ttttaggacc gaggtcgact ttttagaccc
     1081 ctccatgaac ctgccaagct gccaaggtgg ctccattgag gttctctcag cccccaatgt
     1141 gcgcctatgg ggtcgaagcc tcgtgcaggt tcgctgccta aaggctgcct ggctctacaa
     1201 catccccctg atgatccggg tctacgggga ctatgtggtc tctacccgct atttacagcc
     1261 cggcaatcgg ctctctagct cggatatgcg gattattaat ggtgacctca ccagcgtccc
     1321 cgatgacgtc atccggaccc caaaagaggc tgacgatcgg gtcttaaccc gccccatcca
     1381 aatggggatg ccgattgggc taaacgattt acgagaaact gccgttatca aggtaggaga
     1441 tcctgtaacc atccttttga aggggcggga ttttcaggta accggttcgg gaaccgccca
     1501 aacccaaggc atgattaatg atatggttcg ggttaggcta aacgatggac aagtgcttca
     1561 gggcaaggta atccggcctg gagttgttga aatgacctta gaccgttaaa gtgtagggta
     1621 caagcagtca aatgaggtca atgcagtaat aagggataac gatgaagatt aacgacagta
     1681 caccgctgat tccgtctggc aaagagcctg gcagggtaga tgtcgcccaa aaacctccat
     1741 tgcctgctgc ggccgacccc aaagctgctg ccttaaaagg cccagccgtt agtctggata
     1801 tcagcctgac tgcaaaatta gccgagatta aagaagaact caaaaccgat aagaccgcca
     1861 ccgataagct tctcttagat aagattcggg caaagatcga ctctggtgag tttgaaatcg
     1921 attaccaaaa gattgccaac ggcatgcttg gtgacacgat tgctgccctt ggcagtaaag
     1981 tccgttccaa gtaagaccca acacccctaa gacttgaagg cccagaagcc ttagcgtatg
     2041 cttgcatgca ttgcaaaacg aataccatta cgccatgacc tttgctgaac taaagacgct
     2101 acttgaacaa attgctagtg ccgttgcgaa gttgcgacag accttagaga ccaataatat
     2161 ggacgcctta cccgaggcac tggagcaaac gcgcgtggcg cttgagttaa tcaatggcta
     2221 ccctgatggt cctgagaaac tgaaatccga tatcaaccaa tatcccgaag aacaaaagca
     2281 aatcttgcat ggtcttttag atcaagcttc ggttgatcat caaattaatg gagatctcat
     2341 tcgcttggcg atgcagcgca gcgccgcgat gcaatccttt attgcccaac aagctcctgg
     2401 ggcaacctat ggcagcgatg gtgaagtacc tggcgccgta ggcggggtgt tgtctcgtaa
     2461 ggtttaggaa aaacttaggc gatctttaac ttcgcccgaa tccggatcat gccttgggtg
     2521 tggatctggc taatgcgccc tgcagtgagg ttcatgacct gggcaatttc tcggtaggac
     2581 aaatcttctt ggtaatgcaa ggccatgact aagcgctcgt tctccggtag gctctccaaa
     2641 atagtggcaa tgcgatcggc aaactgacgc atcgaggcag cttgcatcgg atccgaatcc
     2701 atatcggcgg ggagcatgtc ttcggataaa tcatccaacg gcatcaggtt gaccatagtg
     2761 cccattaccg cgtgatactc atcaagacta atctcgcagg catcggcgat ttcaatctcg
     2821 gaggggtggc gccctagttt ttgctctaag gagcgagtgg ttttctctag attaacaagc
     2881 ccatcgcgtt ggttgcgcgg caagatgtcg ttgcggcggc atgcatcata gatcgcaccc
     2941 cgaatacggg tgcgggcata gacctcgaaa ttaaggttag gttgtggttc ataacgcttt
     3001 agagcatcga gtaaaccggt taaaccctct tggaccaaat catcgagttc cacattgggt
     3061 ggcagacgcg aggcaatttg ataggcaatc cgctttacca ggggcaaatg ctcggtgatg
     3121 gcgtcgttga ggtcaagcgc ctggtaagca acgggattgc gagggggcat tcagatcaat
     3181 tcagaggaaa caaaacaaaa ataatagaac agaaataaag aactacgcag taatttttgc
     3241 agaaatcgtc ttaaagaaat ccactggcaa gacaaactcg ctgacagggc ctgcaaccgg
     3301 cacactaatc tcattatctt ccggatcaac cacaattcga ctcgccataa acgctttcac
     3361 ccaacctgca aagtgcaagg cttggcgcag ggccgattgt gaggcgtccg caagtttctg
     3421 aaaagccgct tgcccctctt cttcagatgc cccaccaata attaagaccg ggtaactttc
     3481 atcgctttgc aagaccgaca tctcacgcac catcgctaaa gcacgactta gggacttgtg
     3541 atcgggtgag ctcaccacaa agcgtttgat ctgcttgcca taaaaagann nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngacc gaattttggc tgcggtggca
     3721 taccaaaaat gatcatcaat attgcgcacc actttaagta gatcaacccc attactaaac
     3781 gcttgggcta agtcgtagcg ggtggggtat aaaaaccccg acatggttct gcgctcggag
     3841 agcccaaact catcaaccaa taaagttttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctagg caaacgacac agctagactc
     4021 tgccccaaaa atagtgcgca gaccctctgc ttgatcaacc ccaggttgtg caacgctctc
     4081 agccattagg aggttttgat ccagtcacct aaattatt
//
LOCUS       DDPQ01000100            4101 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_30729,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000100 DDPQ01000000
VERSION     DDPQ01000100.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_30729"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    911..981
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1125..1213
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1091 a          863 c          919 g         1068 t
ORIGIN      
        1 ctggtttgaa gcttggtatt gccgaagcgg gtattaagcg agcaaaccgc aaagatatat
       61 tagtaatccg tttagtacct gggtcgagtg tttctggagt atttactcaa aaccgtttct
      121 gtgctgcccc agttcagctt tgtagggctc acttattaga gcgtaaccct attgaggcct
      181 tgattattaa tacgggcaat gccaatgcag gcacgggcga agagggcttg cgtcgagcac
      241 aacaaacttg cgatgcctta gctagtgcct tagacattag ccctgagcaa atcttgccct
      301 tttcaacggg ggtcattctt gagcccttac ccgtagagaa aattattacc gcaataccta
      361 aggcaatctc tgcactcaaa gaggatgatt ggtattcagc ggctgaagct atcatgacca
      421 ccgatactca gcccaaagcc gcatcactca ctattcaaac ttccgctgga ccgatcgtca
      481 tgaccggaat atctaaaggc gccggcatga ttcagcctaa tatggcaacg atgctcggct
      541 tcattggtac cgatgtaaat ataaattcag atttacttca ggatcttact cgcgaggcag
      601 ccgatttatc gtttaatgcg attacgatcg acggtgatac ctcaaccaat gactccttca
      661 ttgtgatggc aactggacaa tcggcagtga gtattcagag taaatcagac ccagcatatg
      721 ccgcatttag ggagggttta attaccctct ctcgtcaatt ggctcagatg attgttcgcg
      781 atggagaagg tgctacaaaa tttataacta tccgagttaa gggtggcaaa gatagtgtgg
      841 agtgcaagcg cattgctaaa tcaattgcgc actcaccact agtaaaaacc gctttttttg
      901 caagcgatcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nttacagttg atttaggtcg tggaaatgtc gaggagacgg
     1021 tttggacctg cgacctttcg catgagtacg tttcaatcaa tgcagactat cgatcataac
     1081 catcataata tttattactg aataataagg ttatttacgg tgaannnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnntttggca gtattggttt tttaaaaccc gttaagcatc tatcggacat
     1261 atcgtttgat gatcttaagc acattgatcg ccagcgagat gcgattattg aaaatactca
     1321 gcattttgtt gataaaaagc ctgccaataa tattcttctg accggtgctc gaggtactgg
     1381 taaatcctct ttgattaagg cctgcttaaa tcagtttgcc aatcaaggtt tgcgtcttct
     1441 tgaggttgat aaagatcatt tggcagacct cccagacatc actgaattac tggcagtacg
     1501 tcccgaacga tttatcgtat tttgtgatga tctctctttt gaagatggtg aatcaggcta
     1561 caaagcgatg aagtccgctt tggatggctc aatctcagct caagtggata acgtcttaat
     1621 ttatgctacc tcgaatcgac gccatttgtt accagaatat atgagggata acgagtctta
     1681 tgttcatacc gatgatggcg agatccatcc gggcgaggtt gttgaggaga aaatatctct
     1741 atcagaacga tttggtttat ggctatcgtt ttaccctccc cgccaagaag agtatttagc
     1801 gattgtctcc cattggctgt ctcattttgg actcacctct aaagaaattg aggctgctcg
     1861 ttcagaagct ttggtgtggg ctcttgagcg cgggtctcgc tcaggacgcg ttgcttggca
     1921 atttgcaaaa cactgggctg ggtcccactc ctcaggtaaa taagactaac aaatgacagt
     1981 tcagtcacga cctattgttg aggtcgcagt tgggattctg ctgaaagagg gcaaagaggt
     2041 cttgatggga aagcgcccag aaggaaagcc ctatgctggg tattgggagt ttccaggtgg
     2101 caaattagag gctggcgagt ctgttgcatt ggcgctgcat cgtgagctta aggaggagct
     2161 ggggatagca atctccctca atccttccca cttttctgag ttgatcactc tagagcatga
     2221 ttatccacac gcatacgtac ggttacacgt atgtttagtg cagcaatggc aaggtgagcc
     2281 tgctggctta gagaatcagg cgctatcttg gcagtccgta ttccactcaa aattgactgt
     2341 tgacccagtc cttccagccg catggccgat gattgagtat ttgcagaatt atttacaaac
     2401 gaaataattt agaaatggca gagtgttaac ttaaacggaa tatcttcatt gatcagcgtg
     2461 ggcttttgat cacggtcgca cttgagaaga cgaatcgata ataagtattt gttggcactg
     2521 atttctgcaa agagcgaagg atcctctaag gtaatacgca ttaactgata gaccttgcca
     2581 gaaggtgctt gttgaaaaga tcctttgtgc gcaagaacct ctctactttc tccagactca
     2641 cgcagcagac gtaaaaataa gtgacaagat tcttgccaag gcagcattgg ataaataaat
     2701 ttctgaagca gctcgcgacg ggatgtcact ggactgtggc gccatgcata aaatccggga
     2761 agatcaatcg ggcttgtgcc ccctggagtg tgtaagcgcg tacgaattgc attaagccac
     2821 tcattatcgg caatgattgc attaggcttg ataaccgagc tattaacttg tgtagcagca
     2881 tgctcaagct caccaagggt tgctaatagc ttatcttgat ctacctcttt agatgatttg
     2941 agaccattta agctaaattt ttggcgttcg aattctttta ataaaagagc tttaatatcg
     3001 ccccgcgcac taatatcccc aagctcaaac aaagtgatga gcgcattgtg atgcagctcc
     3061 gcatcatcag acctggcaaa gtgattaaag cgatcgaata aatactcaag gcgaagcatg
     3121 cttcgcacca gttcgttaaa ggggtattcg taaatgatca caggctatta ttttatgccc
     3181 acccgagcca aaaaaactac aaatccagca ttttttggtg gagcagggac gcttgagcct
     3241 ctaaggcgct gagatccccc tcgttagtca ggatggtatt cgcaatagca agccgctcgt
     3301 cccgactagc ctggcttgcc ataatctgta atatggatgc ccgatccagt tggctgcgct
     3361 ccataaccct ttggatgcgg agctcatcgg gacaatctac taccgcaata tgatctaagt
     3421 gctctttcca tgccccagac tccaaaagaa ggggtaccac aaagacaata tagggcgctt
     3481 gcttgttaat agcctgttgt gtccggtcga tggtttcttg acgaatcaag ggatgggtaa
     3541 tggcttctag aatttgtttg gaattaggat ctgtaaaaac ctgctgacgc attttggggc
     3601 ggtttaagga tccatcccca ttaaggtaat caggcccaaa ttctttcaaa atggcttcga
     3661 tagcaggccc attaggggtg gttatttggt gggcgatttg atcggtatca ataaccaccg
     3721 caccctgctt ggcaagaaaa ttagcaacac tagacttacc ggaaccaatt ccgccagtga
     3781 ggcccaagaa cggaacctta ccttttaaat cgaagagttg attaatcgac ttgaccatac
     3841 tatttcaatg atgccagcaa ttgccaagaa ggggccaaaa ggaaagacat ggccactcga
     3901 taatttatgg tgacgaagcc aaagcagccc tccaatgatg ccaagtaagg atgctactaa
     3961 tagcattaat ggtaaagatc caatgccaag ccaagaacct aaggccgcca gtaatttagc
     4021 atcacccata ccaagcccat cttggcggcg aaattggcga tagatacgat tgatgagata
     4081 taaagcgaag taaccaatca g
//
LOCUS       DDPQ01000101           13766 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_7807,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000101 DDPQ01000000
VERSION     DDPQ01000101.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13766)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_7807"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1929..1953
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3876 a         3320 c         3055 g         3488 t
ORIGIN      
        1 ggatcaactc gctgcttata tgaacgaaag tcttttagct cctcatcgct caagtactct
       61 aatttagtaa ttccgattcc atgctcgcct gagatcaccc catcaagcga tcgcgccaag
      121 gccataatgc gatcaaccga gcgatgggca tcttgtagca tttcataatc atcagagttc
      181 accggaatat tggtatgcac attgccatca cctgcgtgca tatgcaaagc aataaataca
      241 cgcttacgta atatcttttg atgaattgca atgatttctt taaggactaa ttcaaacgtt
      301 gccccgccaa agattatttg taggggtgct aataattgaa ttttccaaga agcgcgtaag
      361 cctccatcct gaatcttcgg aaagtaagcg ttgatatttc ctaaccagtc tgaccattga
      421 tcccgaacac tctggattaa agcttgcgcc tgctgcacgc gatcgccaac catttctgct
      481 tgtgagatgt tttcaatttc ctcattagct cgaatgggta atagcggttg cttgatataa
      541 ctctctaatg catcgagtaa ttgcaactta tttttaagcg atagctcaat attgatttgc
      601 tcaattccat cggtgtactc tcccatacgc tcaagcggga ttacaacatc ttcattaatc
      661 ttaaatgcat tagtgtgctt agcaattgcg gctgtgcggg cacgatctag ccaaaacttt
      721 ttgcgagctt ccgcactgac agcaacaaag ccttcaccga cacggcgatt agccattcga
      781 acaacttcac tcgttgcagc cgccacctct tcttcaatct cgccagcaat atccccgatc
      841 aataccattt taggtaaacc attgcgcttg gactttgtcg aatagcccac tgcttttaga
      901 taacgatcgt ctagatgttc aagacccgcc aagattgggc cgcccttttt gctcaaatcg
      961 tctagatatg ctttgatctc cacaatactt ggaatcgctt cgcgcgcttg tccaaaaaac
     1021 tccaaacaga cagtgcgcat atgtttgggc atacgatgca atatccacgt ggcgcttgta
     1081 atgagtccgt cgcaaccttc tttttgtatt ccgggtaagc ctgacaaaaa cttatcagta
     1141 acgtctttac ccagccctga tttcctaaac ttggcaccat caatctcaag cagttcagag
     1201 cgaattactt ttttgccggg ttcatcatgg ccgtctgacc agatcaattg aaaacgtgtc
     1261 ttaggaacct catgtatttt tcctaagttg tgatctaagc gctcaacatc caaccaatta
     1321 ccctgcgggt cgaccatccg ccaacttgct agattatcta gtgcagtacc ccatagaact
     1381 gcttttttac cacccgcatt catcgcaata tttccaccaa tgcaactcgc atctgcagag
     1441 gtagggtcca cagcaaagac tagacccgcg agctctgccg catccgctac acggcgcgta
     1501 accacgcctg cgccagaata aataacaggt actaaattgt ttaggcccgg tagtgctttc
     1561 ttttcaacca cacctaaatc aattaatttc tcagtattaa taacagcaga cattgctacc
     1621 atgggaatag cgccgccggt atacccggta ccaccacccc ttggcacaat cgttaggcca
     1681 agctcaatac atgctttcac taagctcgga atttctgctt cagtgtctgg ctttaaaacg
     1741 acgaagggat attcaacgcg ccaatcggta gcatcggtca catgtgcgac gcgtgacatg
     1801 ccatcaaaag aaacattgtc aggatgtgtg catttaccaa atatacgctt cgcttgcttg
     1861 cgtaactgct ctaccgtctg aaactcctgc tcaaatgatt gcactgcttt tctggcacta
     1921 gtaattagnn nnnnnnnnnn nnnnnnnnnn nnntgccata acgcatcaat taacgcccgt
     1981 cgacgtcctg ggttgtcgag caaatcatct tgcaagtatg ggttacgttg cactacccaa
     2041 atatctccca atacctcaaa tagcatccta gccgagcgac ctgttcgcct cactccacgc
     2101 aatgtctcga gagtttgcca agcctctttg cccaaaaggc gaattacaat ctccttatct
     2161 gaaaacgagg tgtagttata cggaatctcg cgcaaacggg gagtttccgt atcggtctca
     2221 gacaacctgt ctaaggggat gggagcgttc ataggagctt agctattaaa agcctatttt
     2281 agtagacctt cgcaatttgc gtgagtttgg tctaatacac aatcctgaat caattgaagg
     2341 acttaagtac catccatggt acgctttgag catgaaaagt aactatttaa agcgaatctt
     2401 gagcgccaag gtctacgacc tggctcgaga gaccgaactt gaaaaggcga acaatctcag
     2461 cgctcgcttc cacaacacca ttcttttaaa gcgcgaagac aatcaagcag tgttctcgtt
     2521 caagctacgg ggtgcctata acaaaatggc gcaactgagc ccagcccttt taaaaaaggg
     2581 tgtgattgcc gcatcagcag gcaaccatgc acaaggcgtc gcccttgctg ctgcaaagtt
     2641 aaagtgcaaa gcaattatcg ttatgccggt cacaacccca caggtgaaaa ttgatgctgt
     2701 aaaggctcac gggaaaaaat gggtggaact cattttgttt ggagattctt atagcgatgc
     2761 atacgaacat gcgcagcaac ttcaaacaaa aaagggttat acatttatac atccctttga
     2821 tgatccagat gttattgccg gacaaggcac tattgcccaa gagattctgc aacaacatcc
     2881 cgatacgatc gatgcaattt ttgtcgccat tggtggcggc ggattaattt cgggcgtagg
     2941 tgaatatgtc aaagctgtcc gcccaaacat taaaataatt ggtgtacaaa cagtagactc
     3001 cgacgcaatg aggcaatcac tcgccgctgg taaacggatt gagttaaagg aagtcggttt
     3061 attttcagat ggcactgcag tcaagctagt cggcaaagaa acattcaaga tttgtcagcg
     3121 tgtagtggat gacattgtcc ttgtggatac cgatgagatc tgcgctgcta ttaatgatgt
     3181 atttacggat acacgaagta ttttagagcc cgctggtgca ctagcgattg caggtgccaa
     3241 aaaatacatt gagcaacatc gtcttaaaaa taaaactttt gtagcaattg cctgtggcgc
     3301 taatatgaac tttagtcgtc tccgctttgt tgcagaacgt gcggatgttg gtgagtttag
     3361 agaagctgtg tttgcgatta ccattccaga ggagcgcgga tcctttaagt ccttttgcaa
     3421 aatgctaggc aagcgtaatg tcacggagtt taattaccgc atcgcccatc aagacaaagc
     3481 acatattttt gtaggtatct cgacacaaaa ggcgggcgac aacaaattga ttgcgcagtc
     3541 ctttcggaag gctggttttg caaccattga tcttagtcat gatgagcttg caaaagcgca
     3601 tttacgtcac atggtgggtg gtagctcccc gctagccaag gatgaactgc tttatcgctt
     3661 tgagtttcca gagcgcccgg gggcactaat gcgcttctta gacagtcttg cccctaactg
     3721 gaacattagt ctcttccatt accgcaatca tggtgccgac tatgggcgta ttttggttgg
     3781 tattcaggtg ccaaaaaatg aacaacaggc tttcaagcat ttcttaaaga ccttgggcta
     3841 ccctcattgg gatgaaagca agaaccccgc ttaccgattg tttctcaaat aatatgtctt
     3901 atacgctcga tggcaaattg gtggttgcga tctcctcaag ggctttattt gattttgagg
     3961 aagagaatcg tctgtttgag caaagcgatg acagtgccta catgaaattg cagcttgagc
     4021 gaatagctgc ccctgcgcaa actggagttg cttttccact ggtcaaaaaa ttgttacgct
     4081 ttaaccaaga taagcaacgg gttgaggtgg taatcctctc gcgtaatgat cctgttagcg
     4141 gtttgcgtgt ctttcgttcc gccaagcatc atggacttga tattgagcgt ggagtgttca
     4201 caaggggtag gcccccatac cattacctca aatccctaca ggcaaattta tttctatcag
     4261 ccaatgaaga ggatgtacgc gccacccttg ctgcaggatt tccagcggcg cgtgtttatc
     4321 cagagtccag taaaacggct gaacataatc ctaatgaaat ccgtatcgcc tttgacggcg
     4381 acgcagtact attttctgat gaggcagagc aagtttttca ggatcaagga ctaaaagcat
     4441 tcgttgccca tgaaagtaaa aatgtttcca tcccacttcc acctggtccc tttaagcctt
     4501 tattggcagc gctacatcaa ctgcaaaatg aatctgcgcc caaaagtgag atgcggattc
     4561 ggactgcttt ggttactgca cgctctgcgc ccgcacatga acgagcgatt cgcaccttga
     4621 tggactgggg aattgaggtc gatgaggcta tgttcttagg cggtttatcc aaacgcgagt
     4681 tcttaaaaga gtttgagcca gactttttct ttgatgatca aaccggccat tgcaatgcgg
     4741 cttctagtgt tgcccctacg ggccatgtaa tctcaggagt cgctaatagt gaacgcaata
     4801 aaacttaagc aaagttcgca tgactgaatt cgtccttggg caacaaagtg cctaccccaa
     4861 tcaatacgat ccgagtctcc tctttccgat tgcacgatcc gataatcgat ctaccctagg
     4921 atttaatgcg caatccacgc cacccttctt tggggtcgat atatggaatg cttatgaact
     4981 tgcttggcta aacctcaaag gcaaaccaca aattgcattg gcagaattta tcattcctgc
     5041 cgagtctccc aatatgattg aatccaaatc attcaagcta tatctcaata gcctaaacca
     5101 acattgtttt gctgacttag catccgtaag agcatgccta cagaaagacc ttgccaaggt
     5161 tttgggagcc agtcttcagg tgaaattagt tcagcccaat gaagtcaccg caaacaaaaa
     5221 aatgaatcct attgaagaac ttgaggggca gttattagat cgtcttgaca ttgaaataaa
     5281 tagccatcaa aaagcggatc cacttttatt acaagccgat cacaaatctg ctccaatcac
     5341 tcaaaccttc gtatctcact tacttaaatc gaactgtcca gttaccggtc agcctgactg
     5401 ggccagcgtg caaattcagt atcaaggaag accgattcaa gaggaaggac tactccgtta
     5461 cctaattggc tttcgccaac tcggggaatt tcatgagcac tgcgtagaaa aaatattctg
     5521 cgatatcaag gcgcaatgta aaccagaaaa attatcggta tatgcgcgtt acacacgccg
     5581 tggcggcttg gatatcaatc catttcggac cgattacaac gctgcctggc ctaacaatat
     5641 tcgtcacgcc aggcagtaat ccaacaaggg cttagaaagg aatatcgtcg tccatggcgc
     5701 ctagaccacc gggcgcttga ctaggagtgc tttcaggaga cttggggcga tcaccagcat
     5761 cccctgaacc ggccatacgc gctccgagca tttgcatact gtcagcaatg atctcggttg
     5821 aatatttttc ttgaccgctt tgatcagtcc acttacgcgt acgcaagcga ccctccacat
     5881 aaacctgaga accctttttg agatattgac cagcgatctc agctagcttg ccaaagaagg
     5941 cgatgcgatg ccattctgta ttttctttca tctcacccgt ctgcttatct ttgtaacggt
     6001 ctgaggttgc aacagaaata ttggtgacgg cgtcaccact gggcatataa cgtgtttctg
     6061 ggtcgcgccc aacattacca actatgatga ctttattgac tgaagccatt ttgtctcccg
     6121 aagtaaaaag taaataaaaa gtgtggctaa agctacgatg ccgattgcgc agatggaaca
     6181 ttgcgcattg gccatgcaat tataagccag agaataatca aaacactgcc gccaataaac
     6241 accgataagt aaccggagta ttgcattaat aagccaccaa tagcagcgcc tgcaaataat
     6301 cctacggatt gtgtagtgtt gtaaattccg agcgctgctc ctttttcatc tttggcccaa
     6361 cgtgagacca tggaaggctg caaggcctct aataaattaa agcccacaaa ataaactaac
     6421 agcgcaagca caatcaacgc aagcgaatgc gcttgtaaaa atagtacttg tgccaacaaa
     6481 agaatgacaa cagaagtcag caaaacacga cgccactgtt tctttttctc accataaata
     6541 attcccggaa gcatcaagaa aaaagagata accaccacag ggaaatacac cttccaatga
     6601 tcatcgagtg gtaatcctgc attgaccaga agtcttggga ccaccaagaa catcgctact
     6661 tgggtcgcat tcagcacaaa aacgccaaca tttaagcgca tcagttcagg gcgaaaaaat
     6721 atttgcctca aaggttgagg cgctgcccgt atttgcttcg gtgggttggg caccaaaagc
     6781 catgccacca agaatccaac aaagccgagc cctgctagta ataaaaacat gccatcgagc
     6841 ccaatcgcac gataaatggg tgtcgcaatc accaaagaca tggcaaacga taaaccaatg
     6901 ctgccaccca cgatcgccat agcagcggtc ctcacttgtt cgcgggttaa atcagcaacc
     6961 caagcggata ccgcagcgga gatagcacct gctcccatta agccgcgacc aatcgcgatc
     7021 cacaataaat catctcgact agccgcaatg aatgcgccag caataaatag gctcaggccc
     7081 cacaaaacga ccggttttcg accaattcgg tctgataagg tacccagagg aatatgaaaa
     7141 aaagcctgaa caatattaaa gataccgagc gctaagccta cccagagggc attttgcccc
     7201 cccggtaaat ccttggcatg caaagaaaag acgggtaaga gtaggaaaag gcccagcatc
     7261 cgcaagccaa agatgccagc aagagccagg gttgagcgga gttcggaaga gttcattaga
     7321 aaaggctata ttaacaagtt acgctaaaaa atcatgaaca acgaaattaa gatccgcggt
     7381 gcccgcaccc acaacctaaa aaacattaat ctcgatatcc cgcgcgagaa actggtggtg
     7441 cttacggggc tttcaggatc gggcaaaagc tccctagctt ttgatactct gtatgccgaa
     7501 ggacaacgtc gctatgtgga atcactttct gcatatgcaa ggcagttttt acaactcatg
     7561 gaaaagcccg acgttgatgt cattgaggga ttatcgccag ccatatcgat cgagcaaaaa
     7621 gcaaccagtc acaatccacg ttctaccgta ggcacggtta ctgaaattca tgattacctt
     7681 cgtttgttat ttgcccgtgc aggcacaccc tactgccccg atcatgagct tccactagaa
     7741 gcacaaagcg tatcccaaat ggtagatacc gtattagcga tgccagaaga tagcaagtta
     7801 atgattttgg ctcctgttgt cagcgaacgc aaaggggagt ttgtcgattt atttgaaaac
     7861 ttgcaagctc agggattcgt ccgctttcgg gttcgctcag gaggcggaac aagcaatgcg
     7921 gctaaagcag aaatttttga gtccgatcgc ctacctcagt taaagaaaaa tgataagcac
     7981 tctatcgagg tagtggtaga ccgcattcgc gtaaaagcag aaattgggca gcggcttgct
     8041 gaatccttcg aaaccgcact tcgacttgcg gatggtaaag ccatgatcgt caatatggat
     8101 accggcgatg aaactatctt ttcaagtaag tttgcctgcc ccgtttgttc atactcattg
     8161 caagagttag agccacgatt attctctttc aataacccga tgggtgcttg cccaagttgt
     8221 gatggccttg gtcatatttc cttttttgat cctaaacggg tggtcgcaca cccagatctt
     8281 tctttagcgt cgggcgccat caaaggttgg gatcgacgca atcagtttta cttcaagtta
     8341 ttacaaacct tagcgaaata cggcggcttt gatctggaga agccgtttga aaaactctct
     8401 aagaaagctc aagaacttat tttgcatggc tctggtgaag ataccattcc atttgaatat
     8461 ctcaatgaaa agggtcgctc ggtggttcgt gagcactcat ttgaaggaat cctggccaac
     8521 tttgaacgtc gttatcgcga aaccgactct gcagcagttc gtgaggagtt ggcgcgttac
     8581 caaaatatcc gctcctgtcc tgattgcaag gggacacggc ttcgaaaaga ggcgcgtttt
     8641 gtcaaagtgg gtgaaagtaa acaggcccgc gctatttacg aaattagcgc catgccatta
     8701 cgtgaggcac aaaactattt tgctgaacta aatcttaaag gcgctaagcg cgagattgct
     8761 gacaaaattg ttaaagagat tggttcccgg cttcgctttt taaacgatgt tggtttggat
     8821 tatctttctc ttgagcgcag tgccgatact ctctccggcg gtgaggcgca acgaattcgt
     8881 ttagcctcac aaattggctc gggactgact ggggtgatgt acgtattaga tgagccatcg
     8941 attggtttac atcaacgcga caatgatcgt ctcattgcga ctctcaaaca tttgcgggac
     9001 ctaggtaata gcgttttggt tgtagaacac gatgaggata tgatccgctc ttccgactat
     9061 gttatcgaca ttggtcctgg tgctggtgtt catggcggtc aaatcgttgc gaccggcacg
     9121 ccaacggagg ttgaaaacaa ccctaactca ttaacaggcg cttatttatc tgggctagaa
     9181 gaaattaccg tgcctgagca tcgaaataaa aatgatgggc gtttcttgag catcattggc
     9241 gcaacaggca acaacctcca aaaagtcgat gcgcagattc cagtgggctt attaacatgc
     9301 gtgaccggtg tatcgggctc cggtaaatcc accttaatta atgacacctt atttcatgcg
     9361 gtcgctcaac atctctatgg gtcaagtgct gaacctgcgc cccatgaacg aattgagggc
     9421 atagagtttt ttgataaggt cattaacgtt gaccagtctc caatcggacg aacgcctcgc
     9481 tccaacccgg caacctacac cggggtcttt accccgatcc gagagttatt tgctggagtg
     9541 ccagcagcac gagagcgcgg ctacgaagca gggcgcttct catttaacgt caaaggtggg
     9601 cggtgcgaga cctgcgaggg tgatggcgta cttaaagtag aaatgcattt cttaccagat
     9661 gtttatgtac cttgtgatgt ctgtcatgga aaacgctata accgtgagac cttggacatt
     9721 cgttacaaag gtaaaaatat tcatgaggtt ttggcaatga caattgaaca ggcgcatgag
     9781 ttctttgaag cagtgcctgt ggtcaaacgt aaacttaaaa ccctgcttga tgttggctta
     9841 ggttatgtct gtctcggaca aagcgctacc acgctatcag gcggcgaagc gcagcgtgtc
     9901 aagttgtcac tcgaactatc caaacgggat accggccgca ctttgtatat cttggatgaa
     9961 ccaactactg gtttgcactt tcatgatatt aaattactgc tgggtgttat tcacacactg
    10021 aagaagcaag gcaatacagt ggtcatcatt gagcacaact tggacgtcat taaaactgca
    10081 gattggttga ttgacctnnc agcaagcttt acggggaaat atctgaagcc gttgttaaag
    10141 gctaaatcgc cattaccagc caaaaagaaa aagtctttag agcaagcgag cctcagctaa
    10201 atcgattgat tctgggctac cggataaaac cacaatatca ccttgctgta aaacagggtc
    10261 ctcggcaagg tctaactttg tatagtccga ttcagtcgat tttctgcgta ccgcttgaat
    10321 gttaacccca tcctcgtcca gtgagagctc agaaagtctc ttaccaaggg cttgagcgcc
    10381 ttcttgcaag ggaacagaat ggagatgcca ggattgattt gcgcttacct catcatcttc
    10441 ggcgcctcta aaatacccac gtaataatcc atagcgctct tcgcgagcat ccgtaatacg
    10501 ccgtaccacc ttgcgcatgg gcacgcccat catcaataaa acatgagagg ccaacattaa
    10561 gctaccctcg atgagctctg gaatcacttc agtggcacca gcactttgta gtttttctaa
    10621 atctgcatca tcgcgcgtac gaaccaatgc ggtcatgcca ggctttaaac cctcgaccaa
    10681 gcgcaggatc tttaagctag ccggcgtatc tgcaaacgat atgactagtg cttttgcacg
    10741 agataagccc gctgctacga ggacgttctc acgtgttgca tccccataca ccacatgact
    10801 cccccccgct acagcatctg cgatccggtc tggatctaaa tctagcgcta gataggaaat
    10861 gttctcttgg tcgagcattt tggcaagcgc ctgacccgaa cgaccaaaac cacaaataat
    10921 gacgtgatct ttattgcgaa cactttttgt tgccacctta gttaaagcaa gcgactgtaa
    10981 tagccattca ttacttgcaa agcgcagtgc aatctgatcg ctgtactgaa ttaagaatgg
    11041 ggcgattagc atggatagca acatagctgc gagcacggct tgactaaagg caggatcaat
    11101 catgtgtaag ccgtcaattt gattcagcag tacaaagcca aactcacccg cttgtgccaa
    11161 acacaaacct gtccgaatgg aaatgcccga acttgaacca aatccacgtg cgaggatggc
    11221 aattaagccg aacttaaata ccaagggcaa aacaaataac agtaaaacaa gaatccattg
    11281 ctcatagata actactggat caagcaacat gccaatcgta ataaaaaaca ggcccagcaa
    11341 tacatctcga aatggcttga tatcgtcttc aacctcatga cgatagggcg tctccgatat
    11401 cagcatccct gccaaaaaag cccccagcgc catggataaa ccaaaatgct ccgtcagtgc
    11461 tgacatgccc aatacaatta atagcaaatt gagcatgaat agttcttgcg agcgataccc
    11521 cgctacaaac ttaaaccaac gactcatgac gttttggccg atataaaaaa tcaacactaa
    11581 agccagcgaa attttgacta atgcgattga aatatctgta atgagatctt tgccgctttg
    11641 tgcaagcgag ggaatcaaaa ttaacagaaa gactactgca agatcctgaa ataaaagaat
    11701 accgactaca ttgcgaccct gctctgtttc gagctccatc ctgtctgaca aaattttggt
    11761 gacaatagcg gtcgatgaca tcgataaggc gccccctaat gcaagcgctg cctgccacga
    11821 catggggtaa atccaggcaa tcatcaagcc aatcggtatt gttaaaacga ttgtcaaaat
    11881 aacctggcta gcgcctagac caaaaacgat cttacgcatc gagcgtaatt tatgaatatt
    11941 gaattcaagg ccaattgaaa acatcaaaaa gaccaccccg aactcggcaa gatacttcac
    12001 ggtttctgaa tcagaagcca ggcctaaagc atgaggtcca attaaaacgc cggcagccag
    12061 atagcccaaa atggggggca agccaaaata gcggaaaatg aggactcccg ccacaccaga
    12121 ggctaataaa aatagggtta gttgaagaac gctcggcata tacttactcc atgatagcta
    12181 agaacccacg tgcgcttgag ttggcgtgcg ataccctcga tatcgaagcg gatgctttgc
    12241 gcaaaatgcg agaccggctt gcaaaaacag gccaagatgc ccttgtttta tcggtcgccc
    12301 tcctagagca atgtcgtggt cgcatcgtag tttcaggaat cggtaaatcg ggtcacatcg
    12361 cccgaaaaat tgctgcgacc tttgcatcca caggctcacc ggccttcttt gtccaccccg
    12421 ctgaggctag tcatggtgat ttgggcatgg taacgcggga tgatgtcttt gttgccatct
    12481 ccaactctgg tgagactgag gagttactca cgattgttcc tatcatcaag cgaaccggag
    12541 ccaagctgat tgccctaact ggtgatccaa gctcatccct tgcaaaatta gccgatgccc
    12601 atttagatgt tagtgtcgac aaagaggcgt gtcccttaaa tatggcacct acaactagca
    12661 cgactgctgc acttgccatg ggtgatgccc tcgcagtggc tctcctcgat gcgcgaggtt
    12721 ttaatgccca agacttttta cgatcgcatc ccggcgggcg cctgggacgt aaattactca
    12781 ttcatgtatc agaggtcatg cgtgattttg caaatactcc caaagttttt gagggtagct
    12841 catttcagga ggctttagca gaaatgagtc gccataagat gggcttagtt gtgatcgtga
    12901 atgtaaacaa tcaagtgctt ggtatcttga ctgacggtga tttacgacgc ttgctcgaaa
    12961 agggtgaaga tacaaaaact attcgtcttg tacagatcgt gaccgcaaac cccagaacta
    13021 ttccgcctga tctcattgct gaagaagcca ttgagatgat ggagcaccat cgaattaatc
    13081 atcttatcgt tgcagatgcc aacaaaaccc tcttgggtgc tttaaatctt catgacttat
    13141 ttgctgcgaa ggttatctaa acatgtcggt tgccttcaat gcccatacca ccaatcctat
    13201 aaaagcgcac ccgcaagctc tcgagcgggc cagcgcaata aagatgttgg tgctagatgt
    13261 tgatggggtg cttacgaatg gtcaggtctt ttttgggccc gatggtaaag agatgctcaa
    13321 aggctttgat atacaggatg gctatgggat tcagttgcta caaagtgctg gcataccgtg
    13381 tgctgtgatt actgggcgcc actcaaaaat ggtactagcg cgctgtgatg agctcaacat
    13441 caaacatgta ttcacgggcg taaaagacaa aaacgcagta ttagatgagc tattaaagcg
    13501 agttgcttta aaagcaagtg accttgcggt tatgggtgat gactggcctg atttagccat
    13561 cctcatgcgt gctggttttc gaattgctcc agcacaagcc catctggaag tttgtcagat
    13621 tgcacactat attacccatg ccaaaggtgg ctatggtgct gttcgcgaag catgcgatgt
    13681 gatcttaaag gcgcgcggcc aatatgacca gctcctgatc tctgcaaaag gtcagtcagg
    13741 aacatcatga aattcactag caggca
//
LOCUS       DDPQ01000102            6206 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_3460,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000102 DDPQ01000000
VERSION     DDPQ01000102.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_3460"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    635..681
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5770..5875
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1691 a         1535 c         1282 g         1545 t
ORIGIN      
        1 gcgtattcca gaacttctca aaccaatcat gcgcatcagg attatcaact cgctgatagt
       61 ttgggaacat catggggatc aaaccagcat cgctggcacc ctgtacatta ttttggcccc
      121 gcagtgggtg cagtccagag cctggcttac caatttgacc tgtaatacta actaaggcaa
      181 ttaagcagcg cgcattatcc gttccatgca cgtgttgact cacgcccata ccccacagga
      241 tcatcgaggc tttagattta gcaaactctc tggctacttc acgtaagcgt tctgcaggaa
      301 tcccacaaat aggagccatt gcttcagggc tataaccttt aatattttcc ttgagagcct
      361 caaaattaga agcgcggttc ttgacgaact cttgatcaca caaaccttct tcgatcacgg
      421 tataaatcat ggcgttgagc atggccacat ccgtatcggg cttgaactgc agggtccgcc
      481 aggcgtgctt cccaatatcc gtaatgcgtg gatctgccaa aacaattttg gtaccgttct
      541 tagcagcgtt tttcatccaa gtggctgcga ctggatgatt agcagtcgga ttagatccaa
      601 tcaccataat cagaccagag tgctcaatat cattnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn ntttttggaa tagataagct tcttcattgc ttcctttggc
      721 agagccaaaa ccggcaagag acttcttgcc gtattgatca cgcaaacctt ttaacttgcc
      781 agctgctaaa tctagtgcct cgtcccagct ggcctctcgg aagacttctt tccaatcacc
      841 gggattttta ccagctgact caatcgagct ttcatctttc ttgatcccag gctttcggat
      901 caatggtttc gttaggcgct ggggattatg gacgtagtca aaaccaaagc ggcccttcac
      961 gcacaaacga ctgtgattag ctgggccatc acgaccatcc acgctcacaa tctgttcatc
     1021 cttaacgtta taggtgattt ggcaaccaac gccgcagaat ggacaaacgg aatctacttt
     1081 tcggtcaaca gcctgggaac caatatgggt ttttggcatc agcgcaccgg tcgggcaagc
     1141 ctgtacacac tcaccacagg caacacaagt actcaaaccc atagggtcac tgagatcaaa
     1201 tacgatctcg ctatgagcac cgcgcattgc atagccgatg acatcattca cctgctcttc
     1261 tcggcaagct ctaacgcaac gattgcactg aatacaggcg tccaagttca ctgccattgc
     1321 tggatgcgat aaatctgctt gaggttgatg gcggcgcaag gcctttaact cagggcgaac
     1381 ctgaacgtct aagcgattag cccactcact taactcaccg tgctgctgtt gcagacgctc
     1441 tgcatcgcta tcacccaccc acttatatcc ttcatcaggc atatccgata gcaacatctc
     1501 gagaactaat ttttggctct ttagggcacg ctcgctattg gccttgactt ccataccggc
     1561 agtagctgaa cggcagcaac tgggggctaa ggtgcgctct ccattaatct ccaccacaca
     1621 ggcgcgacaa ttaccatccg gacgataacc atctttgaaa caaaggtgag ggatatcgac
     1681 cccatgacgt ttagcagctt ttagaatggt ctccccgcca tatgcaacga ttgttttgcc
     1741 atcaagcttg aactctacgg tttgcagttc tagttcattt ggattgactg gggcgttcat
     1801 ttaagcgatc tcttctggga agtatttagc tatacaacga attgggtttg gtgctgcttg
     1861 acccaagcca caaatagacg catcgatcat taccgtagat aaatcttcta aggttgcttg
     1921 atcccattta ggggcaagca tcaactgcgc tgctttgctg gtaccgacac gacacggggt
     1981 gcactgccca caactctcgt gctcgaagaa gcgcatcaca ttaagcgctg cgtcacgagc
     2041 cttatcgtga tggctaaata ccatcacagc ggctgatcca ataaaacaac catagggttg
     2101 taaggtatca aaatccaaag gaatggtgtt catagtggca ggcaaaatac cgcccgatgc
     2161 accgcctggc aaatacccat agaaagtatg gccgtcctgc ataccgccgc aatattcgtc
     2221 aatcaactcc tgaatcgtga tcccagcagg agccaacttc acaccaggca acttaacgcg
     2281 accgctcaca ctaaaactgc gcaggccttt gcgatcgtgg cgaccaaagg aactaaacca
     2341 ttgaggccca cgctgcacaa tatcgcgcac ccaatagagg gtctcaaaat tatgttctag
     2401 ggttggacga ccaaagagac cgacttgagc aatataagga gggcgcattc tgggctcgcc
     2461 ccgcttaccc tcaatactct caatcatggc cgactcctcg ccacaaatat aagcccctgc
     2521 tccgcgacga agttcaatca ggggcaatct atacggtggg ttagcctgta gtttttgaat
     2581 ctcattggcc agtagttcac ggcagccatg gtactcatcc cgcaaataga tatagcaagc
     2641 atcaatacca accacttggg ctgctattaa catgccttcc aagaagcgat ggggatctcg
     2701 ctctaaatac gtacgatctt taaatgtgcc gggctcgccc tcatcgatat tgactgccat
     2761 taacttgggt gcagtttgat ctctgacaat ccgccattta cgacccgccg gaaatcctgc
     2821 gcccccaaga ccccgcaaac cagaatcttc cattgccttg atgacagtct ctgggtcttg
     2881 ctgttggttt gccaaattac ttgctaatgc ataaccaccc ttggcacgat aagcctcata
     2941 tccgacatag gcaggcgaga ccacatttgc cttttgctct ggactaacac ctttttgtac
     3001 aaaatcagct ggttcaaact gagcatcatc cttcgctttt ggatgcgtag tttgtcgatt
     3061 cttaatagca tttgatattt tctcgacatc tgcaaataaa accggatatt gatgtactac
     3121 ggcaacgggt gcttgttcac aacggcccac acaaggtgcc gcaatcactt tcaccttcga
     3181 gttacctaaa atggttggta agcgctcaag taaatttttc gctcctgcga gctcgcacga
     3241 gagcccatcg caaacgcgca ccgtaatatc ggccacaggg tcattgccgc ggaccacctc
     3301 aaagtgatga taaaaagttg cgacctcata aacctctgcc attggtaaat tcattagctt
     3361 ggctagcgcc accaaatgat gatcgtgtaa agcacggtac tcgtcattta ataaatgaag
     3421 attttcaatt aatagatcgc gtcgataagg accatcgcca attaaggctt gaatttctgc
     3481 cagcgcaaca tcatccgctt gccgcccctt tagcttactt ttttgtttaa tacgcgcacg
     3541 aagatcatct gcagttgcga tggcaactgc cttgatttca ccggaaggct taggatgatt
     3601 catgcgatat ctgtctctca atcgaaatac gatttcatag gtgcaagatg cctttgtata
     3661 ggcccgagca aactgtaact aatttaattt attcaggtgt ttatgtaatt gatatggctc
     3721 aagttcaagg taataagggt taaccctaat cctctaagac ctatgtaatt gaggaagcct
     3781 aaaactctat tttgagctcg gcaagaatgt attgctgtaa tccatttccc tctcacgaac
     3841 acgggcttta aagcatgtgt catagtcggg cgtaccaatc cgtaatccca cagaggtgca
     3901 atcttgttgg gctgcagact gaactgttcc gcatccacta agaaacatgg tgataaaaag
     3961 aggggctaca agaatcaaac gaattggtct catgatagta atgagtctag cacctaaatg
     4021 gcttgtttta acaaaagaac ggagccagca aacagcaaaa taagtccata ggtctgctgc
     4081 atgcgatgct tacttaaatt gcgatgcgcc ttggacccca accataagcc agcgatcatg
     4141 cacggaatta agatgaggcc caacgttagt atgggcatat gcaagatcaa gcccgtaaaa
     4201 agcatgaaca acagacgcat aaagaccagt accaaaattg ctagagccat cgtagcccgc
     4261 aagcgcatgg gttgattcag gcgcatcgat aaataggatg cataaattac cccacctgta
     4321 gcatatagag ctgtaaagac cccaccaatt gcaccaaagg gaatcgccca ataacgacta
     4381 ataatatgaa tcggcttgga ttgggaacgg gtcagaacgc gtattccatt tgctgcggca
     4441 aatattccta aaaggattaa taaaggcttc tgtggtgcgt ttaagagtaa ggtaatcccg
     4501 atcaccatgc ctactaaggt aaatggaaac aaccatgtca attctttttt atctgcctct
     4561 cgataggatt tgcctcccaa gtaaaaagct gcgcagagat ctagaatcac catcaagggc
     4621 acaacaattt gcaatgggaa aaataaaacc agaatgggca ccgcaatgat ggaggaacca
     4681 aatccagata tcccaaaaat aaaataagcc acaaacacaa ttgcgcatgc agcaattaag
     4741 gagaggggtg caattagctc aaaaatggga atgctttata aggtcaattt atttactgcc
     4801 tagattaaca ccaagcggga tcccgtcttt atcagcaacc gcctctttgg atgagcccaa
     4861 cttaatacgc tctactaggc taggattatt ttgtagcatt aaatctcgtg cgcgacttgc
     4921 tcgcgtagtg gccaattgtt ttaactctgt atcgccaatt acaaagctta gaataatctc
     4981 actccgtaac tgctgataac gagcctcatt atccggtagt gtgattaagc gttgagccaa
     5041 cttaatcttt cccacctgct gaccatacgc tgatttaatc ccagactgaa tgcgcgggtc
     5101 tgaaaggctt ggggtcggta atggttcgct cgggctcaat ttaaaccccg ctgcattcaa
     5161 aatagcgtga tctgctcggg cgcgcgccaa ctccagtttg tcagaaccgg gatcatatgc
     5221 gccgaataac tcaatcgttg cattagggcg ttttactaat acaccagcta atttatcgag
     5281 cttttcttgg tcggcgggta aataggtgct ctcccccacc acggaataaa tgggcgcatc
     5341 actttgcaag cccaataaag atgccaatgc cctaaatgga gcggtgacaa tattactaag
     5401 cactgtgctg atagcctgcc agaccaaacc ggaagcagaa aactccggcg aatcaatatt
     5461 tcctttgata cttaaagaga tatcaatcac attatccgag tcttccaata aggccacagc
     5521 caaccttaac ggtaaacgct ttccctgaaa atcaggaatc tcctcgccca attgaatatc
     5581 tttaatcaca aaacgatttt gaccaagtaa ctcagcatct tttgctttgt agttcaataa
     5641 aagatcgagg cgtccgtcgt taatggaata tccagcatgc tttataaaat aagcattggc
     5701 agtattcagg ggcacattct taaactcaac agagagatca tgatttcgtc tggggtcatc
     5761 aaatgaggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcgag
     5881 aggattgtgc ttgatttaca gacccagccg agtcaacctc aggacttgtg ggctttgcaa
     5941 acattcggcg gatatttgat aagcccttct catcaagatc aaataagaaa ttaggttgat
     6001 taaatattag ctcatcaatc gttaaggcgg tgttaccgct agccaacttt ctaagctcga
     6061 tttgtttgat atcgacttgc ttccactgaa caaagggttg acgtggccta ccctccgtta
     6121 tctccacatc aagtaagcgc aaatcaccac tcgccgacca caaatgattc tgcaactgat
     6181 accgcatctg gccactcatc actccc
//
LOCUS       DDPQ01000103           15650 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2813,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000103 DDPQ01000000
VERSION     DDPQ01000103.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 15650)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15650)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15650
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2813"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT         4101 a         3666 c         3607 g         4274 t
ORIGIN      
        1 aaatggaaag ttaccaccat ttccaaactg aaagcgacca gaaataatca cttcattaat
       61 tgctgaaaaa acagcaatta atgaagtgat tatttctggt cgctttcagt ttggaaatgg
      121 tggtaacttt ccatttacca ccctgtttga caaaaatgtt ccagtcaaat cgattgcgat
      181 cattaatgtg aacttaatgc acgccatctt agtaccattg gattcaccaa ttaaatcgat
      241 cgatgacttt aaaaaccgta aagagccggc aaccattggt attgtgaccg gttcgtccgc
      301 agagttttac attcaagcgg ctgccaaagt acatggcatt gtggttggta aagacattat
      361 tctcaagaat atgcctcctg gtgaacaaat ggcaatgcca aaaggtatcg acgctgtagt
      421 tccgtgggat ccaacgccct caataatggt taatgagcgc aagaatgcac gtatcattaa
      481 tgatagcttc ccctacaata tttatggctc atcattctat gtacgccaag aggtcattga
      541 taacgcgcca gacgtagttc aagcgtttac cgatgcaatt gtggaggcaa cattatggat
      601 tcgtaagaat ccagatgctg cagtaaaagc aatgcaagaa gatccaaatc ttaagaactt
      661 ctctccactc attttgcgtc aacaaattga tgcttataac aacctctata agccaactta
      721 cctccatcca cttccacaat tctggggtag agcgaatgaa actacttacg aatggctatt
      781 tgagaataag agaattcaaa cgaaggcaac ggctgcaacc tatgccgccg cagtcgattc
      841 tcgctttatg gatcgttcct tcgcaaaagt aggctgggcc gttcctaagt taccaccatt
      901 tttaccagca acgtttaatg cgccattgga taaattacca tacccaacgt attcaacccc
      961 gttaaataca actaagcctc aggccttccc tgaaaaaggt gatttaacga aagactggtc
     1021 atttgatggc aaggtattta agaaacaatg acaagtcttt catggctgtt aaatttacgc
     1081 cgattaattc tgctagcggt actcctaagt tgttgggagt tcgctagccg ttttattcta
     1141 gataaaaccc aagcaactct tctacccccg ccaacggggg ttttagcagg ggctgttgag
     1201 ctcgcagtgt ctggtgagct gtggaagcat attcgtgata gtttaaggcg tgagtttatt
     1261 gcgttttgct atgcgagtgt tgcaatcccg cttggcgtga tgatgggttg ttttaaatgg
     1321 attaatgagc aagtggatcc tattattgag atccttcgtc ccatccctcc tatcgcttgg
     1381 atcccattaa gtattttatg gtttggagtt ggtaatacac aaaatcagtt catcattttc
     1441 ctaggaattt tcttcccaat tctgctcaat acaattgatg ctgtcaaaaa tgtggagccc
     1501 aatctaatac gagctgcgcg ctgtttagga gctactagct ggaatgtaat tacgcgcgtg
     1561 atcttacggg cagctttacc tcaaattgtg acgggtatcc gcattgggct tggggtgggt
     1621 tggatggcat tggtggcagc tgaactagtt ggtgcaaatt ctgggctagg gtttttgatt
     1681 aatgatgcgc gcactgtatt acgtactgac ttcatattgg atggcatgct atcgattggc
     1741 atcattggcc taacgattga ccgtgtaata cgttactcag ccaaaaagtt gatgccttgg
     1801 tctcgcgcct taaatgctta atatttgtaa cagaaagcga ttacattaat gtcagcctta
     1861 aaagtagaga atgttagcaa gatctttcca gcagcgaata aaagtgctgc tgtacaggct
     1921 ttgcaaaacg tttcattaga tgtaaatact aatgaattcg tttccatctt gggccatagc
     1981 ggatgcggta aaacgacttt gttaaatctc attgctggtt ttgagaagcc gagctctggc
     2041 cgaattgagt gcgctggtaa agaggtaaaa gagcccgggg cagagcgtgc aatggtattc
     2101 caagactacg ccttgttccc ttggctaact gtgcgcgata acattgcgtt tggccttaac
     2161 ctcaaaaaga tggattccgg ccttatcaat caaaaagtag agcattttgc taacttggta
     2221 ggcctaggag cttttgtcga gagttatcca catcaactgt caggaggtat gcgccaacgc
     2281 gtttcaatcg cgcgagcttt ggcagttgat cccactgtct tattgatgga tgagccattt
     2341 gccgcccttg atgctcaaaa tagggcaatg atgcagcaag agatgattcg gattctggct
     2401 gaggagagta agacagtcgt attagttact catagcatcg aagagtcaat caacctatcg
     2461 gatgtcatta ttgtgatgac gcgtaggccg gggcgtgtaa aggaaattat tcgtgttcca
     2521 gaaccgcgcc gcatggttga ggatacccca agctatcttg aagtccgaaa gaggatccgt
     2581 gatttaatta gtgatgagca tgatttaagc gatattgctt gaatacggtt gattggtttt
     2641 tagtatttct attagcgttc tgcgctcttt tggcgggctt gtttcgtcgc ctttcgggtt
     2701 ttggtggtgc catggtgatg gcgccaatac tcattggttt tttcccttta gtatttgtaa
     2761 taccaattgt gatgtgcctc gaactgcttg gaggtatttg gttagccaaa aattggcgtg
     2821 tagaaaaagg tgatcgacca cgcgtttttc gaatactaat agcttcagcc ctcactcttc
     2881 cgattggcat ctatttaggc caacaaattg atccgcagta tttaaaaatt atcacaagtg
     2941 ttgcggtatt ttgtttttca ctttatttac ttttacaacc acatattcgt ctaacattaa
     3001 gcaccccaaa agatcattta atcggctctg gcgcaggatt tttgctgggc tcctgtggtt
     3061 tcggtgggcc aattgttgct ctttacttaa gtgcgagcac acttgactat cgccatattc
     3121 gcgcaattct atcgcttgtc gtatctggtt tggctctatt gggaatagtt ttttccatta
     3181 tgtttaacga tagtttgtct tggttaccat ggcttggcgt cggatttcct gctttcactc
     3241 tgggatttat atttgccagt tatattgata agatgggttt tgtaaccgaa tccagattgc
     3301 gacgcttctc aatctatttt ttattggtca atgctttaat taatctgttt attagtatgc
     3361 ttttgctaac taaacaaatg acattaccga ccctctagct gatcagtaaa taatattgaa
     3421 attcatttac actaatcctg ttaactatta attatttata actatgtcaa tacctcctgt
     3481 tactattcaa actctccaag cgttttcaga ggcatggaat gcccatgaca ttgatgcctt
     3541 aatgtctttc atggcaccaa actgcatttt tgaaactgtt tcagggccag atgtatatgg
     3601 aaatcgtttt gaagggcaga ctgcagttcg ggaagccttt gcgtcggcct ggaaaaattt
     3661 tcctgatgcg caatggttga acggcaaaca ctttgtcgcg aacgatcgcg gggtatcaga
     3721 gtggttgttt attggaaccg gtttagacgg taagcgcgta gaggcaaatg gtattgatgt
     3781 atttacattt tcaaacggta agatccttgt aaaaaatgcc ttccgaaaaa tgcggacata
     3841 accatgagta cattaagcct tgacaccgca gcacagctat acaaccccag ctttgatcca
     3901 ttggttgacc aaggggttgg gcaagcaatg gcatatgccc ctacatactg ggtagcaagt
     3961 gcaggaacac cccccgaaaa tgatggggcg attactcacg atcttgatgc tgatgttgtt
     4021 attgttggcg gtgggtttac ggggcttgcg accgccttat ttttagcgcg ggaacatggt
     4081 atcaaggcga ctctgcttga ggcaaaccaa acagcatggg gctgcaccag tcgaaatggc
     4141 gggcaaggtc agaatgcaag cggtcgccta tatcgctccc aatggataaa ccgttggggt
     4201 attgatattg ctaagcgcct tgacgcggaa atccatgggg gctttgaaac cttcaaatct
     4261 cttgtatctg agatagattg tgatgcacaa gattacgggc acttgtatgt tgcgcaccgc
     4321 gacaaaaaaa tggcatttct agaaaatgaa gcaaaggtaa tgcgcgacat ctttggttat
     4381 caaactagaa ttcttgatcg tatgcagttg caagaagagt tttgccatga tcaggaggcc
     4441 tgtggagcgc tctatgagcc agagggcgta ggcgtacatc ccctaaagct tgcttatggt
     4501 tatcaacgct tagctcgctc actgggagta aagatccata ctggcagccc ggtgattgct
     4561 tggtcacaac gtaatggaaa gcattgcctt gaaacaccgg gtggcaaagt gcatgcaaaa
     4621 gtagttggtc tggcaacggg cgcttatggc ggacaatttt taactccgca tttacgaaat
     4681 cgcgtgatgc caattctctc taactcaatg gtgacgcgag tattaacgga tgtggagcta
     4741 gctgagattt cggttaaaaa accagctttc attacagata ctcgcacctt gcggttttat
     4801 taccgtcttt tacctgatcg gcgcatgcaa attggtagtc gcagcgccat tcacggcgcc
     4861 aatgccacgg cccccaaaca cttacagtta cttcgagatg gtttagctcg taagtttccg
     4921 atccttcata acatcccggt tgattattcc tggtggggct gggttgatgt atcgcacgac
     4981 atgatgccgc gcattgtcca gcctgatccg aatcagtcag ttttttatgc tcttggttac
     5041 ggcggtaatg gtgtttcatt ttctgctcat gcggggcgtc gtcttgctca acgtatggca
     5101 ggcaaaacag atcctgcatt tgatttgcct atttataatt ctcaattgcc tgggcattta
     5161 tttgcgccat ttcgccgttt gggtcagtcc ctgctttacc gttggtacta tttgcgcgat
     5221 gagatcctgt aaaaaagtgt ttcaagcttt tctattcgcg tttactaagt aaatttccta
     5281 tataaatcaa tatcttaaat taatagctaa aatcaaaata gtaagctcac tttatgagct
     5341 tttgatagat gaaactgcta cctcacaacg tggaggtcag aatgaaaata ttagttgggt
     5401 ggttaatttg gatggttttt gcagagcgcc caggagagtg gggcaggaaa gagtgggtct
     5461 gtcgcattaa ccgtcaatag gcccttttca ccctaacccc tgagcctatt tcaaatgcct
     5521 ttaacaccct aggggcttga aaaaagtcat tgttttgttg caaacttagt ctatattaag
     5581 gagtatgtaa tcgggcgcgc cttgacgtcg acatcaaggc agaaaggggg ggcggtgctt
     5641 agtattctga aattggatgc cggcggaatt ccacagtgct ggattgatgc ggaagacgcc
     5701 acccgacatt acgccgataa cagcgtactc tggaccttgg gtgaccccat tgccgtcatg
     5761 cgtggcggta tgtcacggct caccggtcgc caatcgatta ttgagctgca ctccatcatt
     5821 gctgtaaaag ggtctgccaa gatcaattta tttgatgtgg tgcctgccat caccaaacgc
     5881 aaactttatc gacgtgatcg tggtctttgc gcttattgtg cttgccgtat ctctgaaaat
     5941 gacgcagaag ccgaacatat tgttcccaat agtaagggtg gcagctatac ctggatgaac
     6001 ttagtggtct catgccgtcc ttgtaatcaa cgcaaaggga atcgcactcc agagcgagct
     6061 gggatgagtc tgatttatgc accttacaca ccaagtttgt atgaggacat gatattgaag
     6121 ggcagaaata ttctggcgga ccaaatggat ttcttggcgg ccaacttacc aaaagatagc
     6181 cgaatacttc aagacccatt ttgggtttag tcgattgcat tctgcaatca caatccattc
     6241 catttttctc cgatgaacgg cttacgtatt ctcaagcctc gctcgatcgt tctcattgcg
     6301 cttctcatct ctgtgttggg acacctttat ctttttgtcg ggttccctag ttttttattc
     6361 tccaaagccg ctccgcttga agagaacttt gttgcagagc tacgagtaga gcctattaag
     6421 aaagtaaaac tcagcaaacc agcagcacct gtaccaactg ttcaggaagc aagctcaaat
     6481 gcagtgggcg atggcgcaaa gattgaggcg ggtgatggtg gtcagggtgg ggcgcaagag
     6541 gggcaggcct ttcgcttgcc agactctggt atctattact acgatgccta tctcgatggt
     6601 caatatcttc aaactgcatc gattgaatgg caggttgatc caagcaatgg ctatcgtttg
     6661 tttattaata tcccatacgc attttttggg ccatttatct ttgagtctcg aggcaagata
     6721 gatgcttatg gtttagcccc agacttttat gtggaacatt taggaagccg ccctgcacga
     6781 tttactcgtt ttgatcgtga tggcaaagga ggagggaaga tgttcttctc ccaaaagcca
     6841 gaggagatga aagacctgcc accgggtaca caagatcgcc ttagtctaat gatgcaatta
     6901 gcatccttat taggtggtga cgataagatc gatgaaaagg gaacggtccg tgagataccg
     6961 attgctaatt tagattcgat agaaacctgg cgctttcaaa gccaaggtga agagttatcn
     7021 ngtgaaagaa aaagatttca agcgccgcaa tgatatttgg ctcattaaag attttggttg
     7081 gattccgggt agagttcgtc ttgaaaatga aaagggcaga accattgagc tcttttttaa
     7141 acaattagat ccaatagcag acctaccgaa gtaattcttt attttcctga tttcttgcga
     7201 attatttggc taatcatatt aaagagaata gcgcccgaca tagaggcagc aaagatgcca
     7261 ctaaaggcaa tcatcacggg cagaatcttc caattctcag gcaatgaaaa agtgccatat
     7321 ccaatggtgg tgtacatctc gcccacaaag taaaacgcat tggtatcgac ctcaaatatt
     7381 ttcaaggcaa ttaaagcgta ggtccaagca atcatatcga ctaaatgcgt cataaagatt
     7441 aggcccacag ccaccatata aaaggggacg gacaaccaaa acatcccttg acggtcagcc
     7501 caatcaatca ataactgata gatctttccc actaacaata tgagtaaggc gtggacagct
     7561 aacaaaccaa ttgccaccag aaatacgata tagagttcat cgattgggag gctgctcgat
     7621 acctttgtaa agagggtaaa gaagttaggt aggtcaatca ttagatagat aagacagatt
     7681 tatcttattt gacataataa tgattatacg cagataagcc tagtaggatc tagggcttag
     7741 attcaacaaa aacaggcttg ggtccaccct gatacttcgt cttgtaaagc ttctcccagc
     7801 gggatcgcaa gccctttgag atctcttcct gattaaacaa atctgcgaca tcaatcgcac
     7861 taaagccttg atgattacgg atctgcagat cagctccgcg atccaataac aagcgtacca
     7921 attgaatatt cccggaacga actgccatca ttaagggggt tgtgtcgctt tcagacaaag
     7981 catccacttt ggcgcctttg ctcaagagat aatccgctac cgctaaatgg ccctccgtgc
     8041 aggcgtaatg caagggtgtc catcctgatt tattgatctc ggcgccgcgt ttatccacta
     8101 agacctttac ctcagacaac atgccataaa gggatgccat catcaaaaca gtttcgccag
     8161 agagattggg atggtctaca tcgacacctt tgagcccaat caaataatcc agggtttgct
     8221 tggatttctc cttagcggca tatacggtta atggattgcc gcccttaacc agcaaattgg
     8281 ggcttacacc agcagaaatc agtttcttaa cctcagaaag atcatcaaat tgcactgcct
     8341 tggtcatctt gtcgacctgc tcttgggtct gagcaacagc cgttagactt acaagaagga
     8401 aagctagcag agtaattaat ttgcggtgta atctcatgaa tcacttctat ttaactgaaa
     8461 acattgataa aaattatgag ttgtatgctc agcaatcgac tcaaccgaga cccctcttaa
     8521 atccgctata aagcccgcta catgggatac ccaagccggc tcgtttgttt ggccacgatg
     8581 gggcaccggc gctaaatatg gcgagtcggt ctcaatcaaa atgcgctcta taggcaatgc
     8641 tttgcaagtt gcctggagat ccttggcgct cttaaaggtc acaatacccg agaacgagat
     8701 ataaaaaccc atctcgatcg ctgcttttgc aacctcataa ctctcggtaa agcaatgcat
     8761 taccccacca atccgccctg caccctcctc ctgcataatt ttgagagtgt cctccgaggc
     8821 tgaacgtgta tgaattatta atggcttctt tgcagccaat gcggcccgaa tatgagtacg
     8881 aaagcgttcg cgttgccact ccatcgactc ataggagcgc tcgcccatgc ggtagtaatc
     8941 taaaccggtc tcaccaatcg caatcacctt aggatcttta gcctcctcga tcaaaaactc
     9001 tagagtaggc tcgggcgtat cttcgtaatc aggatgaaca ccgaccgagg caaacaaatt
     9061 agggtgggat tgcgccaaga gtttcacctt ggggaaatct ggaatatcca ctgaaataca
     9121 caaggcatgc gttaccttgg ctcgcgccat attctccaga acttcaggaa gacgttcttg
     9181 gtattctggg aaatcgaggt ggcaatgcga atcaataaac atgaggtcgc attttagtct
     9241 gcaaaaagct gctggtactg caatagtaag gattctagtt gaacccttgt ggataaagga
     9301 tgattctcat ggcgcctagc aagaagcaca agcttccata acttaagcag tttgttcata
     9361 ttgacctgct gggccaaggc ctgaacatgc ttttcatgcc tgcggtaata gcgtgcttca
     9421 cccgcttgct ttgccgactg tatatcaaac aaccaacgct gcatgcatgc caaaagaatg
     9481 ccaaagggtg ctttatggat tttctcagca cagtctatcc atggaatatc gcgcccctta
     9541 cttagcgcct ctaataacaa gcgagtcgca gaactcgcca gagccatgct atctttatca
     9601 tcacttagac ggcgcgccaa gatcacttcc ttggcggcta acggtgcccc gccttgctcg
     9661 tctaggctgg cctctatttc ttctgaacta attttttgct cagacaaaga tcccaaatga
     9721 gtgcttaacc attgcaaagc aattgcacgt tgaggcttag gcagtgctat caactggcag
     9781 cgcgagcgaa tagtaggcaa cactcgatca agacgatcgc ttaggagaat aaaaatagtt
     9841 tgcgcattgg gctcttcaag agatttcagt aaggtatttg cagcatcagg ccgcaaaccc
     9901 tcgaggggat agatcaaaac aacgcgctga ccacctcgat gggttcccac attaatggcc
     9961 tcaattgccc ttcgcacctg ctcgattttg ataaaggcgc tctccttctt tccgtcgccc
    10021 tcctctttgt cttccacttc ctccacttcg ggtttgggca tcccctccaa ttcagcatgg
    10081 ggcatacggt gctgatagag ggctggcacg agaccaataa aatcaggatg gttggcagtc
    10141 tcaaaccagc ggcaggcttg gcattcctta cagggcttta cccccgagga gaagagatcg
    10201 gcctcacaca aaatagcctg agatagctcc aaggcaaagt ctaatttacc aatccccgct
    10261 tgcccatgta gcaagatcgc ttggggaagg ttctcaagat tgaactgctc ccaagcatca
    10321 acatgccagg ggtataccga ccggcccacc tggctttcat aattaggatt ttctatttcc
    10381 atgcgatata gacaaaaact attattaaat aagtcaattt attcaatgac ttagcctagt
    10441 aatccaaacg aatttcttga agctgttgcc agatggcatc tggggtctgt aaggcattga
    10501 tgatcatgaa tcgctctgga tctgctttag ctcggcgcaa atattcaccc cgaaccttct
    10561 caaaaaagct taaatccaac tgctcaaatt tatcggggga gcgtgccttt gagcggcgct
    10621 cttcggcaac attcccaggg aggtcaaaca gcaacgttag gtttggctga ataagacccc
    10681 ccgccctacc ctgcaccaat tgctcaagag cattgagctt ctctaggctc aaaccgcgcc
    10741 ctcccccctg atacgcaaaa ctcgcatcgg tgaagcgatc ggagatcaca atctttcccg
    10801 cctgaagggc aggctctatt acctcggaca aatgctctcg ccgggcggca aacatcaaca
    10861 gggcttcggt ctcaatatgc atggcttgat tcaggagcag ctttcttaac tcctctccca
    10921 atggggtacc cccaggctca cgggtgagaa caatctcttt cccttgatag cgctctcgca
    10981 aatgcttcgc aaaggcctca agatgagtgc ttttacccgc tccatcaatg ccctcaaagc
    11041 tgataaagaa cccagggtaa ttaggcttgg tcactcatta cctcgctggt ttagaggcgg
    11101 acttcgccgg attgcgttgg tagcgatcca ccgccgcctc gtgctctttc aaattagtcg
    11161 agaactggct actgccatca ccgcgtgcca caaaatacag agcattcgat actgcaggct
    11221 gagcggctgc cagtaaggac tctttcgatg gcattgcaat gggggttgga ggcagtccat
    11281 aacgcatata ggtattgtag ggagtatctc ggcgcagatc ggctttctta atatcgccat
    11341 caaactttgg gccaatgcca taaatgacag tgggatctgt ttgcagcatc atgcccttct
    11401 ttaatcgatt ctgaaatact gccgagatca tgcctcgttc gcttgcctga cccgtttctt
    11461 tctcaacaat ggaggcgagg attagtaact gataggggct ttttaacacc ccaccctcag
    11521 gattggcttt ggtgtaaaga tcccaagcgc cagccaattg tttttgcatc gctgcgagtg
    11581 cgcgcttata aatcactaaa tcaatttcat cgggatcaaa aatataggta tctggtagga
    11641 aatagccctc tgcactttcg gcatccaaac ccacagcctt cacaaattgg cttgggtcta
    11701 ggcgattact ttgaagagtc acggcggggt gcgcctccat cgctgcccta acctgccaaa
    11761 tactcatccc ggggatgatc ttgacgctct cccgaattcg atcaccccga gccatttgta
    11821 gcaagacgct gcccaaactg gcattggcag gaaacgcata ggtcccgggt ttgagtttag
    11881 aacccaccca taaagatcgg gcccccacct gaaaaagcca agctggggag ctcatgcctt
    11941 ggtcttgcaa ttgcttagca atgctagaga cgcccgattt gggtaatacc ttaaagcgtt
    12001 gctctgcagt gatgccttct tttggcttgg gcacaacccc ccatgtaaac aatgcaaggt
    12061 aataaagccc aatcagggtc aaggcaatca ggatgcccgt tcgtaattta gagatcaaag
    12121 cagtctagtc aagggtgatg gtattgggga tcagaaagtt tttgttcata ccactatgat
    12181 aaaagtctta gcaaaccgat tcataaacga cacaatacag cccaaatgac cgaacaccaa
    12241 gtaaccgagt tacgtgattg gggactcctt ttggtagaag gcccagatgc tagctccttt
    12301 ttgcaaggac aattgaccaa ctccgtatta ggaatgactc gtacacctcc cgcagcgatt
    12361 gcacaggatc attctggtgt gcgtctctcg ggttactgca ctgccaaggg gcgtttactg
    12421 gcgagtgctt ggatttgttt gtcaactcat gcagagcaag atcgtttcgc cctgtttgta
    12481 tcgcgcgacc tggccgctac gtttgcaaaa cgcttagcca tgtttgtgtt gcgctctaaa
    12541 gcaaaggttg tagatgttag tcatgagtgg acagtctatg gcgtgcttgg tccggcaaat
    12601 acgttacctg caaacttacc gagcgatgcg attgctctca ccatgaccca tccctctggc
    12661 actggtgatc aaaaacgtat tctgtttgcg acacaaacac cacttccatc acaaagcgca
    12721 tcttccgaag atcttggtgc atggaatgct gcggagatag ccagtgcaat accacgcatt
    12781 gttttggcga cccaagatca gtttgtgccg cagatgatca acctagaatc cattggtggg
    12841 gtcgacttca aaaaagggtg ctaccccgga caagaggtgg tggcgcgcag tcaataccgt
    12901 ggcgctatta aacgtcgcct atatcttgcc caagttgaga ccgctcttgc gcttgcaggt
    12961 cctgctaccg aagttgttca tgaggatgat ccaaaccagc cagccggtat ggtggtctta
    13021 tccgcccagc atccttccca aacggggctt gtgaccctgc agatcgagtg caagtctgag
    13081 ttggcccaaa gtggcaaatt acatctcggt aaggcggatg gccctgcatt gcaattagga
    13141 aatctgccat attccctgat tgaaatttaa tcaggcatat tattggtatg tgcctcattt
    13201 tatttgcctg gaaatctcac ccaaagtacc ccttggtcgt tgctgccaat cgggatgagt
    13261 tttatgaacg caatactgcg ggtattggct ggtggccaga acatccccat atcctagcag
    13321 gacgtgatca agccgatgtc attggtagtc ctggtacctg gttgggtctt actaaaaatg
    13381 ggcgcttctc tgccctcacc aatgtacgct cacccagtga gaaaaaaccg gatgcccgaa
    13441 ctcgtggtga actgccactc atgtacctct caggtgccga taaaccagat cactttgttg
    13501 aaaaacatag taagcgtttt gatcagtaca acggctttaa tctcctcatg gcagatttaa
    13561 gtgaccctag caatgcagaa atgcattggg ttagcaatcg ggtggtcatg ggtaaatcgg
    13621 tgcgacctcg caaaacattc ccgacccaat cgctcactcc tggtgtgtat ggactttcga
    13681 atgcaatgct cgatacccca tggcccaagg tgaaccatcg tatcgctgcg tttgcccaaa
    13741 cacttgccat ggatacaggc aatctcaaac acgctgatca atatctgcat ttacttgcgg
    13801 acgatcatga agcaccagat agagaactcc ccaacactgg tgtgagctta gagtgggaga
    13861 aagctttgtc ggctgccttc atacgtacgc aacactatgg cacacgatca agtactgtct
    13921 tgcgcgtgcg caatgatggc aactatgagg tcgttgagcg tcgctttgat gccaatggcg
    13981 taatcgcaca cgatgtgatt accggcaatt taattgggtc agcctgggct aattactcgg
    14041 tgtaagcgca tggcatttcc gctacagggt gaagatacct gtcaacatct cctgcccacg
    14101 ccattgccaa atccttattg ggttggcttt gaaagcaatc ttgccaaaga acttggtatt
    14161 actttagacg catcgggctt accgcaagac ccacgatggt tagaggtctt gagcggcaat
    14221 gctctagaga cccatgaaca tacctttgcc gatccaattg ccacggtgta tagcggccat
    14281 caatttggga tatgggcagg tcaattaggc gatgggcgcg ccattctgct gggtgatcta
    14341 caaggctatg agcttcagtt aaaaggttct ggcatcacgc gctactcacg catgggcgat
    14401 ggccgtgcag ttttgcgttc ctcgattcgg gagttcttgt gcagtgaggc gatgcatgca
    14461 ctgggtgtgc ctactagtcg cgctctagcg gtcaccggtt ctaaaaaatc tgtacttcgt
    14521 gagagtctcg agaccgcagc ggtctgcaca cgacttgccc ccagttttat ccgagtaggt
    14581 cactttgaac acttcgccgc tgcacgctct actgagcatc tcaccaccat ggctgattat
    14641 ttacttatgc atcactatcc agaatgtcaa ggtacacatg atccctatct tgcattgttt
    14701 gccgcgatca gcgaacgtac tgcaaaacta gttgctcact ggcaagcgat tggtttttgc
    14761 catggcgtat taaatagtga caacattagt atcttgggtc tcacaattga ttatggcccc
    14821 tttggctttt tagatcgttt ccaaatggac cacatctgca atcatagtga cggtcaaggt
    14881 cggtatgcct atcaacgtca accacagatc atgcactgga atatggcctg cttagctggg
    14941 gcaatgcttc cattaatcga gactcgctac ggtagcgagc ttgcccaaga gcacttacaa
    15001 aaaactttag aggagttccc agagatctat cgtgccgctt ggttaaaaca ctttaggacg
    15061 aagcttggtt tattgacccc taatcctgac gatacatctt tagtggagtc actcttgcag
    15121 ttgatgcatc acaatcgcgt cgactttact aataccttta gagcgctctc gcattttgat
    15181 gcaagccatg atgctaaatc caaccccttg cgcgatcagt tcttaaatcg ggacgctttt
    15241 gatacctgga tgcaatccta tgtcgctcgc ctcaaggaag agaagtgttc ccatgcagaa
    15301 cgccagtctc acatgctggc cacaaatcct aagtttgtat tacgtaatta tttagcccag
    15361 gcagcaattg agaaagctca aaacgatgat ttctcggaag tgaagcgctt gctagcaatc
    15421 cttcaagacc cgttttccga acagcccggt ctcgaagaat acgccgcccc tccccctgcc
    15481 gcactagagc atattgaggt cagctgttct tcttaatcct tctttacaat caccatcatg
    15541 actaaatcag acgaccaata ccgtcaagaa ctcagcgaga tcgaataccg ggttactcgc
    15601 catgccgcca ccgagccacc gtttacaggt cgctactggg atcattggga
//
LOCUS       DDPQ01000104            3288 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_28821,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000104 DDPQ01000000
VERSION     DDPQ01000104.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_28821"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT          796 a          854 c          762 g          874 t
ORIGIN      
        1 tagaacccct cgagattaac aagaacaatg ggcttggtat acgccttaat gtgattaccc
       61 gtccaaacct caaacaactc ttcaaaggta ccaataccac cgggaagcac tacgaaggca
      121 tcggagagtt caatcatcat ctccttgcgt tcaaccatgt tctccaccac atggagatcg
      181 tgaagccctt ctaaaactgg ctcagcctcc gtcagaaaac ctggaatgat cccaaccact
      241 tttccttggc aagaaagtgc gccacgcgcc acctcgccca ttaagccccg tcctccccca
      301 ccaaaaacga gacgccaacc aagaccggca atttggcaac ccagatcttg tgccgcagag
      361 gcccagttgg ggttcttacc aggccgtgag ccacagaaaa cgcagacggt atattccatt
      421 ccctgattgt gccactgtcg tcccacgctg ctttgattgg cttgttagaa tcacaagact
      481 tcactgatag gaacgctatc caagaatcat gtaccaagca tttgtcgacg ccttttcctt
      541 actggcccag cttgactccg caatcattcg gatcgttcta acctcgatcc aggttagctt
      601 aaccgccctc attcttggaa ccctcatcgg acttcctttg ggcgcatggc tagctactga
      661 agagtttgct ggtaagaagg tggctattgt gatcctcaat agcctaatgg gggtacccac
      721 ggtgatcgtc ggggtcttag tctatttaat cctatcgcgt agcggtccct ttggcgaatg
      781 gggctggtta tttacggtta aagggatgat cgttgcccag ttttttatta cgaccccact
      841 tattgctgca ttgagccgtc agatcttaga agactcgtgg agggttcatc gggagccatt
      901 tatggcctta cgtctaccgc cccttgcgcg tcttaaatgg ctgatctggg actgtcgctt
      961 ctccctcacc attgccatcc tagcgggttt tgctagagcc atctccgaag tgggtgctgt
     1021 aatgattgtg ggtggcaata ttgatcgggt tacccgcacc atgaccaccg cgattgcttt
     1081 agagaccagc aagggtgatc ttgctttagc gctctctctt ggcattgtgc tcctcggcat
     1141 tgtcttactg gcaaatattt tcatatttgt agtgcgccaa attgcggagc gtcgttatgg
     1201 ataaactctt aacactagac caaacgaagg tttttgctga tgggcgttcg atctttagtg
     1261 ctaatcacat tgagatcccc atgaacggca tgattgcctt ggttggcccc aatggagccg
     1321 gcaagaccac cttattgcgc cttatccatg gcttgattaa acctgatgcg ggatcctgtg
     1381 taagcccatt tgataaacat gaaagtgcat tggtcttgca ctacacccct atgctcaagg
     1441 caagtgtgcg cgagcatcta aaactattac gtgacaccaa tattgccgtg agtgatgctg
     1501 acattgatca agcgctagcg agagtaggcc tcagccatct agcaaataat cccgcccaga
     1561 aactgtctgc gggtgagcgt caaaaattat gcttcgcacg agcccgttta caaaacccca
     1621 aattagtcat gctcgacgag cccaccgcta atctagatcc aaatgcaagc gatgatgttg
     1681 aggccatgat gacgcaattg gctcaagaat ctaaggtggt gatatttgca tcccacaaca
     1741 tggctcaggt gcagcgccta gctaatacgg tactctttat gcaagatggc caaatcctag
     1801 aaatcgccac gcctgaacaa ttctttaaaa accctcaaag taaagaggct accaagttcc
     1861 tagcccgaga atttattgca caatagcttt atctatcgtc atcactagga gacctctatg
     1921 cgcctgatta gcaatagctt taagaatggt gaactgatac ccggagaatt tgccttttgt
     1981 atcccgaatg atcgtggcca tgtttgctta ggctctaatc gcaatcccca tttggtatgg
     2041 gatgatgtgc ccgctggcac ccaatcgttt gccatcattt gccatgatcc tgacgtacct
     2101 tccaagcccg atgatgtgaa ccaagagggt aaaacggtac cagcttcttt gccccgaatt
     2161 gatttctttc actgggttct agtcgacatc ccaaaggatg ttcgtgagat ccccgctggt
     2221 aagctttctt cagaggtaag ccccaaaggc aagtctggac ccgatacgcc cattggtggc
     2281 gctcatggta ttaatgatta caccgtatgg ttttcaggcg atgcagaaat gaagggtaat
     2341 tactttggct acgatggccc ctgccctccc tggaatgaca gtatctggca tcactacatt
     2401 tttacgatct acgccctcga caccccatcc ctcgctttag gtgggatttt tgatggcaaa
     2461 actgccctgg cagcacttca gcgtcatatt ctgggtaatg ctcaaatcat ggggcgatat
     2521 accctcaatc ctgagttggg ataaaaaaat ccccaagata aacttgggga tcttggtttg
     2581 cctacctgct gggcttattt cttggcaggg gcaggagccg gcgctggtac ggcggctact
     2641 ggagggacaa atggaggcgg tgccttacat ggtgctggct ctggagttcc agcaacccgg
     2701 tacttcattg caaccgcatt aatcgattga catagttgaa agttgccgac cgcaccagca
     2761 taggctgttt ttgcttttgc tagctctgca gcttctgctt gggctggcgt tgggggtggg
     2821 agtgcagcta gggctacgga gcctcctatt gcacaggctg cgctaatgat tacatgtttc
     2881 attgattcaa ccctttccta attttttata ccaaagttca tgatgctctt tgtcccaggt
     2941 ctcatcgcaa taccctgttt tcatggcatc gatcgaacct tgcataccaa tcgtaccaat
     3001 gtagatgtgt ccgagtgcga gtgcggtcat caaaatcnnt ttttatacca tagctcatgg
     3061 tgctctttag cccaagtctc atcgcagtaa ccggtcttca tggcatcaat cgagccttgc
     3121 atgcctactg taccgatata aatgtggcca aatgctaatg cggtcatgag gattgcgccg
     3181 ataccatgaa caatgttggc gatctgcata gtaccgcgcc agtattcaat ctgaataaat
     3241 ggcacgatca tatcgagtac ccagccagag gccgaaacaa ccgagcct
//
LOCUS       DDPQ01000105            8364 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_6041,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000105 DDPQ01000000
VERSION     DDPQ01000105.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_6041"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    4498..4586
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5300..5321
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5777..5823
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6504..6592
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7087..7157
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7331..7444
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2126 a         1684 c         2030 g         2092 t
ORIGIN      
        1 atcatcaaga tcggagttgg ggctgcttga ttggcccatt tttctttcag cggatttgat
       61 tccatccagt tcctatataa gttggtatac caaggagcgg tattggcagt tcggaggtga
      121 tcggggccta tttcatcggt caagatggca ggatcattac caagactttg caaaacttga
      181 ttgaaatcat cgcgggtaat atttcgatag gccatcagtg attgttacgc ctaagtttgg
      241 ccttgcctat tggactaagc tgatttattt gtaagtcaga ctgagatttg cggtaggacc
      301 atataatttc acaatggcaa ctttaccaag taaaaaacca ccccttcaga gcgggtcgag
      361 cttgaattct cccaaaaggc cgagttcgag ccctttgata aaagccctgc tcatcttggt
      421 ggtcattttg gggatgcttt ttacaatgct ggtggcgtat gcattcatta ttgccaagcc
      481 aaacttaccc gctatttctg cattacttga ttacaacccc aaagtgcccc tgagaattta
      541 tacggcggac aaagtgttaa ttggagaatt tggtgaagag cgacgcaatg tcgtacccct
      601 aaatcagata ccgatacact taaggtatgc agttattgcg attgaggacg atcgttttta
      661 tagccatgga ggcgttgatt actggggcgt tcttcgcgca agccttgcta acttacgtgg
      721 tcgtctatca caaggcgcat cgacaattac gatgcaagtg gcacgcaatt ttttcttaag
      781 caatgagaaa acatttagtc gcaagctcta tgaggtgttg cttgcctggg aaattgaatc
      841 ccagttgact aaagataaga ttctagaaat ttatatgaat cagatttatt taggtcagcg
      901 cgcttatgga tttgcaagcg cctcacaaat ttactttgga aaagaactaa gagaaatcac
      961 tattgctgag gcagcgatgt tggctggttt gccaaaagca ccctccgcct ataacccagt
     1021 gtctaacttc aagcgcgcca agatccgtca agaatacatt ttgcaacgga tgcgagattt
     1081 atcctatatc acgccagcag attacaacaa ggcgattgca gaaacaattc gggtgcgtgg
     1141 tttaggaaat gagtttgata cgcgtgctga ttttgttgca gagatggcta ggcaactttt
     1201 gtattctgag tatggggata ttatttactc tcaaggtatc agcgtttaca ccactgtctt
     1261 aaaagctgac caagatgcag cttatagtgc gcttcgaaaa ggaatatttg actatgactt
     1321 gcgccatgcc tatcgcggcc cggagggatt tattgagctg cctgctgata tcgtaaagcg
     1381 ccagcgagca attgataccg cattacttga ttatcctgca ctggatgaat tgcaatctgg
     1441 tgttgttctt gaatccagcc ctaaagaact caaagtcatg attgcaacag gggagcaaat
     1501 taccctatct ggtgatgcgc ttaagttggc acgcgcctct ctctccgaga ccgcgcaacc
     1561 taagcagcgc ataagacccg gggcaattgt tcggttatta agagatgagg ggatgtggaa
     1621 aattgcgcaa cttccccaag tcgaggctgc atttgtcgcg atggatccaa gcgatggagc
     1681 gattttagct ttggtaggag gctttgactt taggcgtaat aaatttaatc gagttacaca
     1741 ggcccttcga cagccgggct ctgcatttaa gccctttatt tacgcggccg caatcgagaa
     1801 gggatttagt cccagtaccc tcgtcaatga cgcacccata tccattggag gctcagaaac
     1861 tggtagtcaa gcatgggagc ctaaaaacta cgatggtaag tacgatggga tgatgcgctt
     1921 gcgtgccgcg ttagcaaagt caaaaaattt agtttctgtg cgcattatcc gtaggattgg
     1981 accttcttat gcccaagaat ttattcaacg ttttggattt gagccagaga aacatccgcc
     2041 atacttaacg atggcattag gtgctggttc taccacccca ttacaaatgg ttaatgcata
     2101 tagtgtattt gcaaatggtg gatactttgt gaacccatat ttaattgata ggattacaga
     2161 ctcaaagggt caaatcttat ttcaggcgga tcctgtcaag gttggaaaaa atgcaaatcg
     2221 agttttagat gcgcgtacca cttttgtagt cgatagtttg ttgcaagagg ttaccaagac
     2281 cggaaccgca gcaagtgctc gccctcgctt gggtcgaaat gacattgccg gtaagaccgg
     2341 aaccacaaac gactccaacg atgtctggtt tgcaggttat aacccacgcg ttgccgctgt
     2401 ggcatggatt ggttttgata agcctaagag tctgggagac cgagaaaccg ggggcggcct
     2461 tgcattacca atatggatta actttatgtc gattgccctt cgaggggttc cagaactccc
     2521 gcgtacgcca cccccgggcg tcgttgaatt agatggtgac tgggcgatac ccgatttcgc
     2581 accctcaggc ggcattcgcc aattgaacta attagactat ggctcgcaaa gcacgctttg
     2641 tgattccagg ccacgctatg catgttttag tgcatggcca taatcagcaa actatttttg
     2701 taaatgaact tgattgcaaa cactatttag attggctgcg tcaggctgca aaagattttg
     2761 gatgcgccgt tcatgcgttt gcactgatgc ctaatcacgc acatctttta ttgaccccat
     2821 ataaagaaga gtcgttggcc agaacaatgc aatcactcgg acgtcgctat gcacagtact
     2881 tcaatcaaag tcgtgcttac tcgggaacaa tgtgggctgg acggttttgt tcttccttaa
     2941 ttgatcctgc ttatgtctta cgttgccaac gctatattga gctcaacccg gttcgctctg
     3001 gattggagat tcggccagag gactcaaaaa atactagttt tgcaactcat attggcggaa
     3061 aaggagagac ttggttgacc gatcacccta ctttttgggg cttgggcaat accccctttg
     3121 aaaggcaatt ggcttgggct agctttgtgc gagaaggcgc ccccaatcat gaggatcaga
     3181 cgataacggc ccatattttg cgttcaaagc cttggctgag ccctgaattt agagctaaaa
     3241 accagaaatt gggtttagac gattggctta taaaacctcg gggtaggcct aaaaagaact
     3301 gaatctgtcc cctttttagg atgaatcatt aatctatccc atataaaaac gggacagaga
     3361 caattaattt ttattgcatc cctaagtaga ttcacctata tttactctcc cggctaaaaa
     3421 tagaccagtc catggggatt gggcgccgca ttgaacgaat ttggatccaa gacaaatgaa
     3481 tcatttagaa ctccgcccaa cagcgcaagg actatataac ccaaatcatg aacatgacgc
     3541 ctgtggcgtt ggatttgtcg cgcacatcaa gggtaaaaaa acgcacgaga ttatttctca
     3601 aggattaaag attctggaga acttggatca tcgcggtgca gttggtgcag atccgctaat
     3661 gggagacggt gccggcatct tgatacagat accagatgcg ctctatcgag ctgaaatgaa
     3721 aaaacaagga gttaatcttc caccagttgg agagtatggc gttggcatga tctttttgcc
     3781 caaagaaagt gcttcacgat tggcatgtga gcaagagctt gagcgaacag taagacaaga
     3841 gggtcagatc gtattgggat ggcgtgatgt acctatcgat gtgaagatgc caatgtcgcc
     3901 cacagttaaa aagactgaac cagtaatccg ccaaattttt attgggcgcg gacgcgaaat
     3961 catgaccacc gatgcactag agcgcaaact gtatgtcatc cgcaaaacgg gtagccacgc
     4021 aattcaggat ttgcacctaa agcatggaaa agaatatttt gtggcatcga tgtctgctag
     4081 aacaatcgta tataaaggat tgctcttggc aagccaagtc ggcgcatatt actgcgatct
     4141 gcaagatagt aaagcagtat ctgcgttggc attagtacat caacgctttt caacgaatac
     4201 tttccctgcc tgggagttgg ctcatcccta tcgtttaatt gcgcataacg gtgaaatcaa
     4261 taccgtgaag ggcaatgtca actggatcaa tgctcgaaca ggtgcgatca gttccccagt
     4321 tttaggcgac gatctacaaa aactatggcc actcatttac ccagggcagt ctgataccgc
     4381 atcctttgat aactgcctag agcttttagt gatgtcgggt tatccattgg cgcaagcaat
     4441 gatgatgatg atccccgagg cctgggaaca gcacaccttg atggatgaaa accgccgnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnngatg atgatctggt cattatggct tcggaggctg
     4621 gagtgttgcc aataccagaa aacaagatcg ttcaaaagtg gcgtttgcag cccggcaaga
     4681 tgttcatgat cgatatggag caaggacgca ttattgatga cgtagagtta aaggactcgg
     4741 tatccaaagc taaaccttat aagagctgga tcgatgcagt acgggtaaag ctcgatgagc
     4801 tcgatgtcag caaaaaagat cttcaagaag aagaaaaaca tattcgtcct gcagcaaatc
     4861 tattagatcg tcagcaagcc tttggctata gccaagagga tttgagatat ttgatggcgc
     4921 ccatggcaca aaacggtgaa gaagcaattg gatcgatggg caacgacagc cccttggcag
     4981 tcttatccga tcgcagtaag tctttgtata actacttcaa gcaattgttt gcccaagtga
     5041 ccaatccgcc gattgatcca attcgagaaa acctggtgat gtccttggta tcttttgttg
     5101 gacctaaacc aaatctttta gataccaaca acattaatcc accaatgcgg ctagagatta
     5161 gtcagccggt attagatttt gatgatatga ccaaggtgcg ctacatcgag cattacacag
     5221 gggggaagtt ccgctcctat gagcttgata tttgttatcc attagcgtgg ggcaaagagg
     5281 gtatcgaagc acgtttagcn nnnnnnnnnn nnnnnnnnnn ngtgagtgat cgtcaggtgg
     5341 atgcccagca tatggctata ccagcgctac tcgcgacttc ggcgattcat cagcatttgg
     5401 tagagcgtgg tttacgaacc agcacgggct tggtggttga gactggtagt gctcgcgaag
     5461 ttcatcactt tgcattgcta gcgggctatg gtgcagaggc gattcatcca tatttagcta
     5521 tggaaactct cgctgaaatg gccaaaggct taccaggcga tttatcgccc gagaaggtaa
     5581 tcaaaaactt tgtgaaagcc atcggtaagg gtttgcagaa ggtcatgtcg aagatgggta
     5641 tctcaaccta tatgtcgtat accggttcac aaatctttga agcgattggc ttaaataatg
     5701 agttaattaa tcagtacttc aagggtacct catcgaatgt cggtggtatt ggtgtgtttg
     5761 aggttgcaga agaagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnngggggtg agtatgcctt ccgcattcgg ggtgaaaaac atatgtggac cccggactcg
     5881 attgcaaagt tacagcatgc gacacgcagt ggccccgaga aaggctatca gacctacaaa
     5941 gagtatgcca atctcatcaa tgatcaaagt aaacacttaa tgaccctacg tggtttattt
     6001 gagtttaagt tagacccgag taaagctatc gcattagacg aagtggagtc ggctaaagag
     6061 atcgtcaagc gttttgcgac aggtgcgatg tccttgggct ctatctccac tgaggcgcat
     6121 gccaccttgg caattgcgat gaatcggatt gggggaaaat ccaatacggg cgagggcggt
     6181 gaggacccta atcgttacaa gaatgaactg aagggtatcc cgattaaaaa gggcgagacc
     6241 cttgctagtg ttttgggcaa ggatgtcgtc gaggcggata ttcccctgca agatggtgac
     6301 tcattacgct ccaagattaa gcaagttgct tcaggccgtt ttggtgtaac cactgagtat
     6361 ttgcgctcag ccgatcaaat tcagatcaag atggctcagg gtgcaaagcc tggggaaggc
     6421 ggtcaactgc ccggcggtaa ggtctcggat tacattggta agttacggtt ttcagtacct
     6481 ggagtcggct taatttctcc accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcagcggg
     6601 tgttgctaaa gcaaagtcag atcatgtggt gattgctggc catgatggcg gaaccggtgc
     6661 ctcaccccta tcctcgatta agcacgcagg ctcaccatgg gagttaggcc tagcggagac
     6721 tcaacaaact ttggtgctga atcgcttgag gagtcgaatc cgagtccagg cggatggtca
     6781 aatgaaaact ggccgtgatg tagtaatcgg tgctttattg ggcgccgatg agtttggctt
     6841 tgccaccgca ccgttagtcg ttgagggctg catcatgatg cgtaaatgcc atctcaatac
     6901 atgccctgta ggcgttgcaa ctcaggatcc cgagctacgt aaaaagtttt ctggcaaacc
     6961 agagcatgtg gttaattttt tcttcttcat tgccgaagag gtgcgcgaga tcatggcaca
     7021 gttagggatt cgaaagtttg atgacttgat cggtcgcgtg gatctcttgg atacccgtaa
     7081 aggggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnngat gtacccgcta aggagcctcg tcatcaaatc ctcactcaag
     7201 atcatggctt aggttctgca ctggatcata ttttgattga gaagagtgag cccgccttag
     7261 agcgcggtga gaaggtgtca ttcatcgtac caatccgtaa cgtgaatcgc actgcgggcg
     7321 caatgctatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnncgtgtg gttgtgcgtg cgccgcatga gttccggggc gatacatccc aaaatattat
     7501 tgttggcaat accgtccttt acggtgcgat cgagggagag gctttcttta atggagttgc
     7561 tggcgagcgt tttgcggttc gcaactcagg ttcggttgca gtggtagagg gcacgggtga
     7621 ccatggttgt gagtacatga cgggcggcac tattgtggtc ttaggtaaga caggccgtaa
     7681 ttttgcggcc ggtatgagcg gtggcattgc gtacgtctat gatgaagatg gcctttttga
     7741 gaagcgttgc aataccagca tggccagcct tgaaaaagta ttgtcgtcat ctgagcaaat
     7801 aactgcaatg ccgaagtctg cttggcatgc ccccgtaaat gtgaaagagg gtggcgagcg
     7861 tcaaacggat gagcagatcg taaagactct gatcgaacgt catttccgtt acacaggatc
     7921 cgagcgagca aaagctctct tagcagattg ggagaggtcc cgtacccgtt ttgtgaaagt
     7981 gttgccaacc gagtacaagc gcgctttagg agaattgtgg gagcgcgctc agggccaact
     8041 cggtaaagag aagtcggtca cggtatagcg ggctagttta agacaagaac atatttaaga
     8101 aattacagga gtttgtatgg gtaaggtgac tggatttatg gagtttgaac gggtcgatga
     8161 aaaatacgaa gcccctgtca agcgcattca tcattacaaa gaatttgtgg cggcattatc
     8221 tgatgccgat gccaaggtac aaggtgcacg ttgtatggat tgtggcattc cgttttgcaa
     8281 taacggttgc ccagtgaata acatcattcc tgactttaat gatttggttt atcagggcga
     8341 ttggaaaaat gcgatagagg tttt
//
LOCUS       DDPQ01000106            3596 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_1190,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000106 DDPQ01000000
VERSION     DDPQ01000106.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_1190"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    513..560
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1807..1940
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          771 a          824 c          857 g          962 t
ORIGIN      
        1 ttactctcgg gtcatgcgcc tctcaatcaa ttaggcaaag gtgcgttcca agaaatggcg
       61 caagccgata ttgcagcacc attgtgcaaa gcctcttgga cctgtgcggg ccctgagtac
      121 ctccaggcag attttttact tgcttgtacc attgcgcaag caggccgtcc tggccccgtt
      181 catttaagtt tgccgtctga tgcgctggaa ggtaagttga cgcaggcttc agcaagtcct
      241 agtccatcgg cccctcaatt acccgcccca caattactct ctgctgaaca tgccaagagc
      301 atcatcgccc aattgcatgc tgccaagaaa ccagtgatct tagtgggccc acggggctgt
      361 catcgctacc ctgctctttt gcaagaacta agtaaggcat cgggtgttcc ggtgattgca
      421 acggagagcc cgcgcggtat aggcgatcct agcttaggag cctttgccga ggtcttagcg
      481 caaagcgatt gcgttttact cttggataag aannnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn cgttccaaga atgccttagg tgcgcgcttg gtcttagctg
      601 cacaggctga tttagaaccg tcggttcatg ctttagtgaa tgccactaag agcgccagca
      661 gcaaagtaca cgatgctact tggttgtcgt gggtaaaaga agcgattgcc tatcgtcctg
      721 ctgcgtgggc cactgctgtc tcgtctaccc ccaatcatgc acacccaggc cgtgcgatgc
      781 tcgccttgca agctatttta gattcgcatc cagactctgt attggtggct gatggtggtg
      841 agattgggca atgggcccaa gcgtgtttgc atgctcctaa tcgcatcatt aatggcatgg
      901 ctggttcgat tggggcagct cttccttttg caacggccgc cgcgtacaca aaacctggcg
      961 ttcctgtggt tgcagtgatg ggcgatggaa cctttggttt tcattcttct gaattagata
     1021 ccgcagtccg gtatcgcttg ccatttatcg gggtcgttgg taacgatgcc tgttggaatg
     1081 ccgagtatca gatccaagtt cgtgagtatg gcaaagagcg tgccaagggt tgtgagttac
     1141 tcccctcgcg ctatgaccaa gttgcgattg cgtttggcgg tgcaggttca atggtgaccg
     1201 atgctgagca ggtcttgccg gctgccaagg ctttgcagtc acaaggctta ccgtctattt
     1261 tgaacatctt gattgagggc ttgccggctc ctactttgaa gagggtttag accccctgtc
     1321 atcctagcgg cctatttagc gttttaggta tatgaacatc ttctaccggc cccgctttat
     1381 tcagtccttt gtgactgtgt tggcatctgc ttgtttgttg gtggcgtgtg ccaatacgag
     1441 tcgtcctggt gtggtcggtg ttgagcgttc tcagttcatg atgatctcag cctctcaaat
     1501 cgatcgcatc tcagcgagta gttttgagca gcaggctaag gctgcccaaa agaagaacat
     1561 tttgattaca agtggtcctc agtatgagcg cctcaagacc atcgctaatc gtttgatacc
     1621 gcagaccgct gtctttagag atgacacgca ttcttggaat tggggcttgc agttaattga
     1681 ctctcccatg gtcaatgcga cctgtgctcc tggtgggcgt attacgtttt ataccggcat
     1741 tattaataag ctcaacctta ccgatgatga gattgctgcc atcatgggtc atgagattgc
     1801 gcatgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn caatatgttt tggtcttgcc taactcgcgt cagaatgaga
     1981 aagaggcgga tgcgattggt ttagagattg ctgcgcgcgg cggctacgac cccagggctg
     2041 caattacctt gtgggagaag atgagtaaag aaagtaaagg taagaaccct cctgagtttt
     2101 tatcgaccca tccctccaat gagaatcgca tcaaagagct ctcagctctc atgcctcggg
     2161 tgatgccttt gtatgaggca gctaagaagc cttaattgaa gagaaaaaaa atgggttacc
     2221 gaagtaaccc aaaagttgga gacatgcttt attgattgtt atgcgttata tatgttttcg
     2281 cttttcaacc attcctaaga acttctcata ctctttctca tctacagaat agctgtgatc
     2341 atcatctgga aatgatccag atttcacttc cgagatgtat tccttgatgc ctttaacagc
     2401 tacctctgtt aagttcgcat agcgttttgt gaacttgggt ttgaaatcag agaagactcc
     2461 caagagatca tggcatagaa ggatttggcc atcggtaccc acccctgctc caattccgat
     2521 ggtcggaatc tcaagttgct tagagataac cgttgctatt tttgcaggca ctgcttcaaa
     2581 ctccaacatg aagcaaccgg catcctgaat ggcaaaagca tcctcgagga ttttcatagc
     2641 ggcatccgct gttcttcctt gaatacgaaa gcctccaaag acagcaacgg tgtgtggtgt
     2701 catgccaatg tgcgatgctg ttggtatgcc tgcgtctacc aatgccttca agatatgtgc
     2761 ctgtgatttg ccaccctgtg gcttaacagc atccgatcct gcctcttgca taaagcgcgt
     2821 tgcattctgt aaggcacgat ctaccgtgtt gtaactttga tacggcatac aacctaatac
     2881 gaatgtattg ggggcgccgc gacgaatcga tgtggcatgc atcaccatca tatccatcgt
     2941 ggcaggaatg gtattgctgt ggccatgggc aatcattgcc aagctatctc ccaccactgc
     3001 aacgtctacc ccagcagcct cagcccattt tgcggatgtg tagtctggaa cagacatata
     3061 caccatcttc tcgccagtct tcttagactc acgtatgtct tgaagattgc gtttcttttt
     3121 ctcgggcgtt gttacggcac ttgtattcaa tgtgatctcc tattcattcg ttacatcatg
     3181 gtattttctg catcctcttt ttgctgcaat gagaagatgg atgaatatcc gcccgaccaa
     3241 tcgggtggga ttaagcaagg caccggatcg tgatgggcaa attgcgcgcg attaccgctt
     3301 aagattgctt gatcatgttt gggtttaagt gggtttacgg tgtatggaaa tgcatcggcc
     3361 gaggttaagg tgtacagcaa gagaggtcga ccttggtcgg actgattggc tggggagcca
     3421 tgtaccgtac ggcaattatg taatgtgagt gatccggctg gtcctgttag gtaaacagct
     3481 ttctcggtat ggatgttcct gacatcatca tctgatagac agcctaccca ttttccatgc
     3541 tcatcgtatt gactcagcat tggttctagc aaatgactct tcggaatgac ccccag
//
LOCUS       DDPQ01000107            4998 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_17535,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000107 DDPQ01000000
VERSION     DDPQ01000107.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_17535"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    4096..4153
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1257 a         1127 c         1315 g         1241 t
ORIGIN      
        1 tattgtgaac gatcctgctg catacggcat tgtgttgcca gaggtagaga accaccccta
       61 ttttgttgcg attgatgtta cccgcgatat tgatgtggaa gtggctgcac aactagcaga
      121 gatgtccttg gcagatttca gaaatctcaa tcctgcgttt aataagccgg ttatcttggg
      181 ggctgcagat caacaaatgc ttctaccgtt tgcgcgggct gaactgtttc agatgaactt
      241 ggctagttat accaagccac tgtcctcctg gaccgcttta accatcacca agagtgagac
      301 cgccgagcaa ttgtccaccc gtctaaacct gaacccgcag ttgattcggg atgtaaatga
      361 cattccacgg gggatacgga ttcgggccgg ttccacggtt ttgataccta agccccccgg
      421 aaaagactcg gatattcctg tgcatttggc cgagcatggt caattaaagt tggataaggc
      481 tgcatcctcc aaacccaaga ccagtcaggg aaataatgca aaggcaagca ctgctaaacc
      541 aaaaacagcg agtactcagc agaaatcccc atcgacaaat tcttcaaaat cagcgaaaac
      601 aggaagcaat acaggctctg ggatgagtgc tggcgttaaa acctctcctt agaccaaagc
      661 ccatgaccac cacttgtttt gagaggaatg cggtatgagc accggttaca aatcatttca
      721 cgagagttcg ataaaggacc ctaaaggatt ttgggcagag caagccaaat tggtcgattg
      781 gcataagccc tttgatcagg tctttgatta ttcgaatcca ccgtttgcaa aatggttcat
      841 tggtggacaa accaatctct gccataacgc agttgaccgt catttaaaag atcgtgcgca
      901 gcagatcgca ttggttgcag tatccacgga gaccgaacaa gagcgcacct atacttttga
      961 ggagttacac gctgaagtca atcgcatggc tgcgatcttg caagcgcatg gggtcgtgaa
     1021 aggtgatcgt gtactgattt atatgccaat gattgcacag gcgtgctttg ctatgttagc
     1081 ttgcgcgcgc attggtgcaa ttcattcggt ggtattcggt ggctttgcat cacacagctt
     1141 ggcttcgcgc attgatgatg ccaagcccaa aatgattatt acggccgagg cggggatgcg
     1201 cggcggcaag gcggttccct ataagccatt gttagatgaa gccatcacgc tagcgagcta
     1261 taagcctgaa aaggtcttga ttgtgaaccg cggccttagt gattacagta ccgtagccaa
     1321 tcgtgatatt gattatgcga gtgagcgtga gaaacattta agtagcaagg tgccagtgga
     1381 gtgggtcgac gccacgcatc cttcttatat tttgtatacc tcaggtacaa ccggtaaacc
     1441 gaagggcgtg caacgcgata ctggtggtta cgccgtagcg ttggcaagct cgatgcgctt
     1501 aatctttggt ggtaatcccg gcgagaccat gttcaccacc tccgatattg gttgggtggt
     1561 aggtcacagc tacattattt atggcccatt aattaatggc atggccacga tcatgtatga
     1621 aggaacgcca ttacgacccg atgcgggcat ttggtggagt ttggtagaaa agtacaaggt
     1681 ctcggtaatg ttctctgcac cgacggcagt acgagtgctc aagaaacagg atccagcatt
     1741 cttgagtaag cacaacttat ccaccttgcg tactttgttc ttagcaggcg agcccttgga
     1801 tgagcccacg gcatcatgga ttcatgatgc actcaagaaa cctgttgtgg ataactattg
     1861 gcaaacagaa accggctggc cgattttggc aattcagcgc ggcgttgagg tgatgccaca
     1921 caagtttggt tccccaggtg tcccggtata cggctacaac atgaagttat tggatgaggc
     1981 gaccggggca gagcttggtc ccgataagaa gggtgtagtt gcgattgaag gaccattacc
     2041 accgggatgt atgcaaacgg tctgggggga tgatcagcgt tttacaaaaa cctattggga
     2101 aacagttcca ggcaagcagg tgtattccac attcgactgg gcgattaaag acaaagatgg
     2161 atatttcttt atcttgggac gcaccgatga tgtgatcaat gtcgcgggtc atcgtcttgg
     2221 aacgcgtgag atcgaggaaa gcatctcaag tcacgccaat gtggcagagg ttgcagtggt
     2281 tggcgtcgaa gatcaattga agggacaggt agcgattgca tttgtaatcg ccaaagatcc
     2341 tacaaaacta gccaatatgg aagctgaagt gatgaagacc gttgactcac aactcggggc
     2401 agtcgcaaga cctgcccgcg tttatttggt gagcgcctta ccaaaaactc gctctggcaa
     2461 gatcgttcgc cgtgcattac aagcagttgc ggaagggcgt gatcctggag acatcagcac
     2521 tatggaagat caatcggtct tggctcaaat taagcaagtg attgggaagt agttagttac
     2581 aggctctcag agctttttgt gagtaagcat tagtcacttt gccgagtgag cggcaaggaa
     2641 ctggtatcat tacgcctatt agtaactatt acatttgccc tagcgcttaa agacacttac
     2701 ctccccgcaa tttagcctcg ggtatgtctt ttggggcaaa aagtgttgga cggcaacagg
     2761 gtattggaga ttgtttgtcg cccttgcttc cttcttttcc ccccgctgta ttagcactcg
     2821 cagatggttc catcttttct ggtcaaagta ttggtgcccc tggtgaaacc agtggcgagg
     2881 tagtcttcaa taccgcactg accggttatc aagagatcat caccgacccc agttatgcca
     2941 gacagttggt cacattgacc tatccgcaca tcggtaatgt tggtgtgaat gctcaagatg
     3001 cggagtcaag tcaggtgtat gccgccggct tgatcatcaa agatctctct gcacgtgttt
     3061 ctaatttccg ctcgcaagat cgcttggatc attatttgcg cgaagcgaag gtgcctggta
     3121 ttgctggtat cgatacccgt aaactcactc ggattttgcg tgatcggggc gcgcaatcgg
     3181 gtgcgatcgt agctggacag gtgggtgata atttagaaca actcacaaaa cgcgccttag
     3241 cgcttgctaa atcgtttgct ggaatggcag gactcgattt agcccaagtc gttacaaccc
     3301 aaaaatcgta tgagtggcaa gaaggtgagt ggcaattatt cggtgagcat ggcaagcccg
     3361 cctatcaaat gatgaagaac cctagcaagc gagtcgtggc atacgacttc ggtgtgaagc
     3421 gcaatatctt acgtatgctc gcacagcgtg gttgtgctct aaccgtggtg cctgccaaga
     3481 ccccagtagc cgaagtgctt gccttaaaac ccgatggtgt atttttatcg aacggccctg
     3541 gggatccaga gccttgcgac tatgccatta gtgctgcaca gagcatcatc gccaagaaca
     3601 ttcccacctt tggtatctgc ttaggtcatc agatcatggg cctagccgcc ggtgcgaaga
     3661 ccttgaagat gaagtttggg catcatggtg caaaccatcc ggtgaaagac ttagatactg
     3721 gtcgcgtcgc catcacatcg caaaaccatg gatttgcggt ggatgcaaag accttgccga
     3781 atgatctgcg agtgacccat gtttctttat ttgatggctc attacaagga ttggcttgga
     3841 aagataaacc agcgttttgt tttcaggggc atccggaggc atcgcctggc ccccatgaca
     3901 tcgcttactt atttgatcgt tttatccatc ttatgaacac cgccggcaaa ggggctcaat
     3961 aatgccaaag cggactgata ttcatagcat cttaattatt ggagcagggc cgattgtgat
     4021 tggccaagcc tgtgagtttg attactcagg cgctcaagca tgtaaagcat tacgtgaaga
     4081 gggctatcgc gtcatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnattactt gggaagtggt cgaacgcatc attgcgaccg agaaacccga
     4201 tgccatattg ccgaccatgg gcggtcagac ggcacttaac tgcgcccttg atttgcatcg
     4261 ccatggtgta ctcaaaaaat acggttgcga gttaattggc gcatcgcctg aggcaattga
     4321 taaagcagaa gatcgccaga agtttaaaga tgccatgacc aagattggtt tgggctctgc
     4381 aaagtctggt atcgcgcatt cgatggaaga ggcgctgacc gttcaacaac gcattcaaat
     4441 agagaccggt agctcaggct ttccggttgt aattcggccg tcgttcacga tgggcgggtc
     4501 gggcggtggt attgcctata accgcgaaga gtttgaagag atctgcaaac gcggtcttga
     4561 tctttccccc actcatgagc tattgattga agaatcactc ttgggttgga aagagtttga
     4621 gatggaagtg gtgcgtgatc gtgccgacaa ctgcatcatc gtttgctcga ttgagaactt
     4681 agatcccatg ggcgtgcata cgggcgactc gattacggtt gcgcctgcac aaacactcac
     4741 cgataaagag tatcaaatca tgcggaacgc atcgattgcg gtattgcgcg agattggggt
     4801 ggacacgggt ggttcaaacg tgcagttctc gattaatcca gtggatggtc gcatgatcgt
     4861 gattgagatg aacccacggg tctcgcgttc atcagcattg gcatccaagg caacaggctt
     4921 cccaattgcg aaggtggctg cgaagctggc agtgggttac accttagatg aattgcaaaa
     4981 tgatattacg ggtggtgc
//
LOCUS       DDPQ01000108            6549 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_3048,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000108 DDPQ01000000
VERSION     DDPQ01000108.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_3048"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2041..2116
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4561..4586
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1704 a         1581 c         1608 g         1554 t
ORIGIN      
        1 ccgcttacct gaatcgactt attagagccg tctcgccctg taaattgcca acggtgatca
       61 ccctgaaccg agctgtagat cagagcaatg tgcttactca gatcgctggg gatggaaggg
      121 gtgccgtttt tctcgagata ctcgggggtc gccaccacca cccaagggtt gagacccaag
      181 aaccgagcac ctaaggagga gtcagagagt cggcccatcc gaatggccag atcaacgcct
      241 tcttcaacca gattaatata gcgatcgtct aggttcaggc tgacctgtaa ttgagggtgc
      301 tgccccatga actgtaagac cagtggagtt aagacccgac gcccaaaggc taccgagcta
      361 ctaatcatga gttttccctg tacctcggat tgcaagaggc cggcgatgtt ctcggcatcc
      421 tcgagctcgt gcacgatggt cttgcacttc tcgtaataga tcttgccaat ctccgttggg
      481 gtcacccctc gcgtgctgcg atggagcaaa agggagccta ggcgcttttc gagagcggcc
      541 acatgcttgg tagcggtggg ttgcgtaatg cctaaactgc ctgcggcttt actaaaagaa
      601 cccgtctcaa ctacccgaat aaaaagagaa attccttgaa tccgatccat aagctaatca
      661 ttataaagga aaaattacca tatattccaa aatggaatag gtgatatagg ggtatctatc
      721 ttctcagaat atctattaat aaggatgata gcgacatctt attcaaaagg aggcaatgat
      781 ggcactcatg aaagccgcaa tggcagcagt tttggtaatg gaaaaagaag gtgtaaccac
      841 tgcatttgga gtacccggtg cagcaattaa tccactctac gcgcaattgc gtgagcgtca
      901 atcaatcact cacatcttgg cccgccacgt tgaaggtgca tcgcatatgg ccgaaggtta
      961 tacccgtgcc aaagccggca acattggtgt gtgtatcggc acatcaggcc cggctggtac
     1021 cgacatgatt actggtttgt actccgctac cgctgactcc attcctattt tgtgcatcac
     1081 cggtcaagca ccccgtgcac gcctctataa agaagacttc caagccgttg atatcgagag
     1141 cattgccaag ccagtgacca agatgtcggt aacggttcgt gagccaggct tagtgccccg
     1201 cgtattccaa caagcattcc atgtgatgcg ctctggccgt cctggcccgg tcttgattga
     1261 cctgccaatc gacgtgcagt tagccgagat cgagtttgat atcgacacct atgagccatt
     1321 gccagtctac aaaccatttg cgaacaagaa gcagatcgag aaagccatgg agatgttgat
     1381 ggctgccgat aagccattga tcgtttcagg tggtggcgtg atcaatgccg atgcagcaga
     1441 tctattagta gagtttgcag aactcaccaa cattccagtg atcccaaccc tgatgggctg
     1501 gggcacgatt ccggatgatc atccattaat ggccggtatg gttggcttac aaacctcgca
     1561 ccgttatggc aatgccacca tgttggctag cgactttgta ctgggcattg gtaatcgctg
     1621 ggcaaaccgt catacgggtt ctttggaggt gtacaccaag ggccgtaagt ttgtgcacgt
     1681 ggatgtggag ccaacccaaa ttggtcgcgt gtttaacccc gactacggca tcgtatcgga
     1741 tgcgaaggca gcgcttgagc tatttgtaga agtagctaaa gagtggaaag ccaaaggcaa
     1801 gttaaagaac tacagcgact gggttgcccg ttgccaagag cgtaagcgtt tgatgttacg
     1861 taaaaccaat tttgataatg ttccgatcaa accacagcgc gtgtatcaag agatgaacga
     1921 agtgttcaag cgcgacacca tttatgtatc aaccattggt ctctcacaga tcgcaggcgg
     1981 tcagttcttg catgtgtatg gtgcccgtca gtggattaac tgcggacaag caggcccatt
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnaaca acagctacct cggtttgatc cgtcaagcac agcgtggctt
     2161 tgatatggac tactgcgtcc agttggcctt tgataacatc aacgtgcagg acgagaacct
     2221 caaaggctat ggcgtagacc acgccaaagt agttgaaggc ttaggctgca aatccttacg
     2281 tgtaaccaac cctgccaaga tccaagaagc attgatcgaa gcgcagaaga tggcaaaaga
     2341 gttccgggtt ccagtggtag ttgagatcat cctcgagaag gtcaccaaca ttgccatggg
     2401 taccgagatc aacaatatca atgagtttga ggatattgat tgccgtcacc cagcgggtac
     2461 tgagggattg gttactgccg gtcttttaga ataactccct aaacctaatc aacccataaa
     2521 ggaactggaa tgcccaagtt tgccgccaat ctctccatgt tgtacaacga agttccattc
     2581 atggagcgct ttgataaagc aggggcggca ggctttaaag cagtcgagtt cctctatccc
     2641 tatgcattct cggcagccga tatcaaagct aaattggata gcaatggttt ggcactggtg
     2701 ttgcataaca tccctgcggg tgattgggat ggcggtgagc gtggcattgc ctgtttacca
     2761 gatcgggttg acgagtacag agcgggtgtt gcgaaagcaa tcgagtacgc caaagccttg
     2821 ggtgtgccac aacttaactg cttagcaggt aaagcacctg ctggagcaga ccgcaaagtc
     2881 ttgcacgata cctttgtggc aaaccttaaa tacacggcag cagagtttaa aaagaatgga
     2941 ctcaagttgt taattgagcc catcaacacc tatgacatcc ctggcttctt cctgtcgact
     3001 actgctcaag gtattgcgat cttggatgaa gtcggtgctg ataacgcctt cttgcaatac
     3061 gacatttacc atgcacaacg tatggaaggt gaacttgcca acacgatgca aaagcacctc
     3121 gcacgcattg gccatatgca gttagccgac aacccaggcc gtaatgaacc gggtactgga
     3181 gaaatcaatt acgcattctt atttaagttc atcgacaaaa tcggttactc cggttggatc
     3241 ggttgcgagt acaagccggc tacaacgacc gatgctggtt taggctggct gaaatccctt
     3301 taagccttgg tccagccaag gtaatttcta ttatttctaa gcagaaggta ttgatatgag
     3361 cgcacaaaag ttaggcttta ttggtttagg catcatgggt tcacccatgg ctggtcattt
     3421 attaaaaggt ggccatcagg tcttcgtcac aacccgcagc caagtccccg aagatttaat
     3481 caaagccggt gctgttgctt gcaagaccgc tgctgatgtt gctaaacagg ctgacatcat
     3541 tttcacgatg gttcctgata ctccggatgt tgagaaagtt ctctttggtg agaacggcat
     3601 ctcctcaggt atcactaaag gcaagaccgt catcgacatg agctcgatct caccgatcgc
     3661 caccaaagag tttgctaaga agatgaacga tctaggctgc gactatgttg acgctcctgt
     3721 atcgggtgga caagtcggcg cacaaaatgc cacgctctcg atcatggttg gtgccgatga
     3781 gggcgtattc aataaagtga agcccctctt tgagctcatg ggcaagaaca ttaatctcgt
     3841 tggtggcaat ggagcagggc agaccgccaa agtagccaat cagatcatcg tggctcttaa
     3901 tattgaagcc gttggagaag cactcttgtt tgcagccaaa gccggtgccg atcctgctaa
     3961 agttcgtcaa gcactcatgg gcggctttgc aagctcaaag atcttggaag tgcatggcga
     4021 gcgcatggtc aatcgcacct ttaatccagg gttcagaatt gagttacatc agaaggatct
     4081 gaacttagca ctgagcagtg ctaaagcctt aggtgtatca ctaccaaaca ctgcaactgc
     4141 gcaagagtta tttaactcct gtgcagcaca tggtggcaag gcatgggatc actccgctat
     4201 ggtgaaggcc cttgagaagc ttgctaactt tgagatcgga caaaaggctt cataagaaat
     4261 gcccacacct aaactcttga ttgtttatct tcacgggttt aggtcgtccc caaattcttc
     4321 taaggcacaa ctaacaggag aggcgattgc ctctctcgtt aaagacggtc aagccatcga
     4381 atggtattgc ccccagttac caccatcccc ccaagcagca atgacgatgg tcaaacaaaa
     4441 aattgcagag actaaggcga aagatctgtg catcattggt tcatccttgg gcggcttcta
     4501 tgccacctac cttgcagagc aatacccgca cgccaaagcc atcatgctga accccgctgt
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnngacg agccctttga ttggcgagca gagtatgtcg
     4621 agcaacttaa agagcaacag gttgaagaga ttaccaatgc agagcgctac ttcttattag
     4681 cagcgaaggg cgatgagcta ctcgactggc atgagatggt agaacattac cctggagcaa
     4741 agcatctcat catcgagggt agtgatcacg gaatcaccga gtacccgcag tatcttgatc
     4801 aagtggttgc gtttgcggcc cacgcttaac gcttcacccc agaacgcaaa gctttcttga
     4861 ccttccgaaa gtgatcctca atcgcttggg taatgttctt acgcccatga ttcttgcgtt
     4921 ctaacaaacc tacacgtcgg taaaccgctt tgcgaccagg tatgctgatc acaatgcgtt
     4981 tatctttatc ccactgcaca ttggcaagca tcggcacaat tgcatagcca agaccctgtc
     5041 gcaccaactc aatgatggcc tcaaccgagt taaccaccat actgctctca atctgcaagt
     5101 gattagaccg aatcacttga tcaacgagtt caccggtcca ggttttgggt tcaaagcgga
     5161 taaagggtaa atcggtacga tctttatcct tgggataaat caggatcatt tgttcttcat
     5221 agagagctgt ccattgcacg cttttgggaa gagcgtaggg tgactcggtc acaatcgcag
     5281 catctaagac cccagcgatt acctgttcta aaaaatcact agatagtcct gtgctaattt
     5341 tgatctctag ctctgggtat tgctgcttca gttgctcaag ggtcttccca aattgcccca
     5401 tcaaacttga gaccagcgcc cctagggtga gcgagccgct taggcccgac ttaaactctg
     5461 cgcctaaaga ctcatagcgc gagatgatct cctgaatcgg ctcgatcaaa gcccgacctt
     5521 gattattaag aaccaaggag cgcttattgc gatcaaagag cgcaacccca atatcaccct
     5581 caagctgggc cagttgttga cccgcagcag cgggggttaa gccgatctca cgcgcagcgg
     5641 cagcgacgct agggtggcgg gtgacagcaa taaagttctt gagaacgcgg atactcgaca
     5701 tagatcaatt ataaagtaat tgtttatctc aattaaaata ctattcgata taaattttac
     5761 tatctatccc tataatgagc ccatgagtca atcgatccta aaagtaaaag tctggcgcgg
     5821 cacccaagat ggtggcttcg tagattacga agtgccccgc gcagctaacc aaacggtgtt
     5881 ggatgttgtg acctatatcc agcgcaaact cgacccaaca ctgagctatc gctttgcttg
     5941 ccgagtgggg atgtgtgggt cctgcgctat gactgtaaac ggcaaagccc gttggacctg
     6001 ccgcacgcac gtggccaaga ttgcgaatag cgacaccctc gagattgcac cattggataa
     6061 cttaccaatc atcaaagatc tagctaccga tatgagcgag ttctttgaca agatgagtaa
     6121 agcggttggc tactttaagg gagagcaaac tcgccatgat gacttcgcca aggtcaaacc
     6181 cgattcggaa gaacgccaac tagccaatgc cggaatcgag tgcattggtt gtggtgtttg
     6241 ctacgcctcg tgcgaagtgg tgtcggcccg tgagtcttat ttaggacctg cagccttaaa
     6301 tcgtgcttgg accttagtga acgatgagcg tgatagtcag cagcttgatc gtctgcgcgc
     6361 ggttgccggt gatgcaggtt gtcactcgtg ccacacccaa ggaacttgtt cagagcgatg
     6421 cccgaaacaa attgagccca ccgctgggat tgcgggtctt aaaaagattg tgggacgtgc
     6481 cgccgtgatg ggtagtaaat ggggcaagct gtgagcgcag caatattgca agccaaactt
     6541 tggtactac
//
LOCUS       DDPQ01000109           14175 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_4368,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000109 DDPQ01000000
VERSION     DDPQ01000109.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 14175)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14175)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14175
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_4368"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    325..422
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1505..1613
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1949..2039
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3721..3848
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10320..10408
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3600 a         3035 c         3358 g         3665 t
ORIGIN      
        1 cctggattaa tgatgatgaa actaacccca tctttttttg ccttttgaac gcgatcaatg
       61 agttcgccct catggttgct ctgataagtc tctaaggaaa tatcagcctg ctttgctaag
      121 tcagccagtt ttttatgaat gtcgtcgagg gtaatcgtgc cgtagacctc tggctccctc
      181 atgcctaata gatttaaatt aggcccctgg attacaagaa ccgatcgatt aacagaattt
      241 ttttgcatag atcgctacag ttattgaaaa ataccaccaa atttaggtag gaactaaggt
      301 aaatcgtgaa agatgaagta taccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nntcttttat catctttcta agctcatctt cagatatctt gcctaattta cggcttttta
      481 tctcaccttt ggggttgatg actacagtaa atggtagggc cccagagggg ttgccaagca
      541 ttttattgat ctcattcccg ccaagccccc ctaaaacaat aggataggaa atgatcgttg
      601 tttctataaa ttgacgaata ttagagggtg aatcgatacc gataccgaca attaacatat
      661 ttttagggtc atattcccct tgaattttgt ctaaagcagg catttcttcg acgcagggtg
      721 ggcaccatga agcccaaaaa ttgacgacta ggaccttatt tttccaatcc actgttgaaa
      781 ttggcttttg gtctggcgaa aaccattccc gttcaaaaag tgccttgaca gcctcatgcg
      841 aggccgagcc aacgcttgtt ctccagttag ctatgagggc gccagcggca gctgcaatga
      901 tggcgatact gccaccgagt aaaaattgac gacggttcat ggagctcctt ggctaaaatc
      961 ctcgcatgca tatacatatc ttgggcattt gcggaacctt tatgggtggg atagcggcaa
     1021 ttgcccgtca agcaggtcat caagtgaccg gttgcgacgc caatgtttat cctcccatga
     1081 gtactcagct tgaggcccaa ggaatagaac tgatcgaagg atactctccc gatcaattac
     1141 aacaatttaa atcgaaacca gatttatttc taattggcaa tgtggtgtcg cgtggtaatc
     1201 ctctgttaga ggagatccta aaccaaggac tgccttacac ctctggtccg cagtggttgg
     1261 gcgagcatgt tttaaggggt caacacgtca tggcggttgc tggtactcat ggcaaaacca
     1321 caacaactgc gatgctcacg tggatattgg aatttaatca acaaagcccg ggttacttaa
     1381 ttggtggggt ccctttaaat ttcacggtat cggctcgcct tgggaagggc gcttactttg
     1441 tgattgaggc tgatgagtat gacaccgcct tttttgataa acgcagcaag tttgttcact
     1501 atcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgattg
     1621 agcgtggctg ctggagccca ttagagcgtt ttggagaaaa gataggcgat tggtcaatgg
     1681 tagacctgct gaatgcagaa gggaagccag gtgctgattt tgcagttcta cagagagggc
     1741 aaaaatgcgc tgaggtccat tgggcaagca actctggggt gatgggcaag cacaatcaaa
     1801 tgaacgctct agctgctata gcagcggcaa accatattgg tattcatccg gaagatgctg
     1861 cgaaggcgct ggctgagttt aagaatgtga aacggcgtct tgagttaatt ggtattaaga
     1921 atcatattca tgtttatgat gattttgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     2041 gctgctgatt tggtctttgc atatggcgca attagtggca aggatgctct gggctgggac
     2101 ttgcaagagg ctatcgctcc tttagcggag cgcggtaaaa gttttcagga tttaggtcgc
     2161 ctcgtagaga gtatttgtac atctgcaaaa ccgggcgatc atattttggt aatgagtaat
     2221 ggtggctttg gtggtgtgca tcaaaaaata ttgcagcgct tgtctgaagt agaaatcaga
     2281 aacagtaaaa aggagtagtg atgcgattac ccaataaagt ggctttaatt acgggcggtg
     2341 ccaatggcat tggttttgct accgccaagc gctttgatga agagggtgcg aaggtcatac
     2401 tgacggacct taatgcgaat gcggtcaaag aagcggcatc tcaactgaag caggccgagc
     2461 cccatgtaat gaacgttacc gatcgcgcta gtattcaagc ggtggtcgat gaggtgattg
     2521 ccaagcatca gcatatcgat atattgatta acaatgcggg cattactcaa gatgcccgct
     2581 tggtcaaaat gactgagcag caatttgatg aggtgattga tgtcaactta aagggtgtat
     2641 ttaactgtac gcaaattatt gttccccaca tgcttgaggc aaaaaaaggc tcgattatca
     2701 acgcttcaag cgtagtaggt ttatatggca attttgggca gaccaattac tcagcttcta
     2761 aattcggcgt aattggattt accaagactt gggcaagaga gtttggtcag aagggcattc
     2821 gcgtcaatgc agtttgccca ggttttatcg ccacagagat ggtgaaggca atgccagaag
     2881 atattttgca agggattgag aagcggtctt ggatgtcgcg cctgggatcc cccgaagaga
     2941 tagccaatgt gtacctcttt ttagcgagtg acgagtcaag ttatgtcaat ggcgtgacca
     3001 ttgaagccag cggcgggatt tctctctaat gcatcttctg tatgaggagg gtggcgatat
     3061 acgtgcggct accgttttat cttcctcggg ctctggtgat acgcagtctt ggcaggttag
     3121 tgcactatcg ggcaagcatc ttaagattaa ggcaaaagag gtatggctag aatttgaaaa
     3181 gcccgacgca caaacgctca ttagcaacgc caatcaattg gcccaaacga ttgatttaca
     3241 gttgttatgg gattgcgcac cggatgaaga gtttttgttt tcaacgatag ctaaagaata
     3301 cttcggagat gcggtatcaa ccgaacaact cgtcgccttg gccattgcat tacaaggagc
     3361 ccccgtctat tttcgtcgca agggccgcgg aaaatttatg cgggctccgc ttgagcaact
     3421 ccaggccggt ttggctgcga ttgagcgcaa gcgaaaagaa ttagaacagc aagcgttatg
     3481 gcagtcagac cttgtggcag gccaaatgcc tgaggcatta cgaccgcagg tgaatacatt
     3541 gctattctcg cctgataaaa acagccttgc gtataaagcc tttagtggag cttgtgaaca
     3601 gaccggcgag catccagccc agttaatgat gcgttgtggg gcattggatt cccccctgac
     3661 ttatcaccat ggacagttct tacgccagca ttttccgggc ggggttggat tttcggcaga
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnca agtactacgg agattgatga cgctttatct ctaacgccta ttgaacacgg
     3901 gcattatcga ttgggtatac atatcgctgc accaggtcta ttgatacaga aggatgatcc
     3961 atttgatcaa gtggcccgcc agcgaatgtc taccgcctac tttccgggca ataagatcac
     4021 catgctccca gaaagcttta tccattattt ttctttagac caaggaaaag tttgtccagc
     4081 agtttctctt tatgtagaaa tcagtgctca gggagagtta ttagatcaat tacctgaaac
     4141 caagatagag ttagttccca tcgaggccaa tttgcgttta gatgatctgg aggagagtgt
     4201 caatgaagag tctttgatgg atcccgccgc aggcctaccc tatcaaaaag agttatccat
     4261 cctttggaac ttggccaagt ttttacatag caagcgtcaa gagcaacgtg aaaaaaatgg
     4321 cttgcgggtt gagcaactcg gtattagtga taccaatgcc ttagcacgag actttaattt
     4381 ccatattagc gctgatcaaa gtgtcgtaga aatagagcca cgcttacgcg gttctatttt
     4441 ggattcgatc gttgcagaat gtatgatttt gtgtaatcgc atttggggac agcagttagc
     4501 agagcacggc ctgcccgctc tatttagaac ccaaaagggt tggggccctc agcgtacacg
     4561 tatgcaaaca actccgggac ctcatgaggg cttgggcctt gatttctatg catggtgcac
     4621 atcccccttg cgacgctatt ctgatttatt gaatcaatgg caattaatag cactcgtccg
     4681 aaatggagta accgcaaaga tggtggcacc attctcgccc aaagatgcga ccttgatggg
     4741 gattgctgct gactttgaga acgtttatca acactatggc gagcaccaag atcgaattga
     4801 aaaatactgg tgcttacgat ggctttccca gcaaggcttg cccaagttaa tacatgcccg
     4861 tcatcttaaa gaaggaatgt cccgccttga gcctatcccc ttgcatttac cgattcctga
     4921 gctggccaat caagcgagga tggctcgggc taagattgag gttatggata tcgatctatt
     4981 gcaattgacc gctgccgctc gtttggtcga gattgaaagc cttcccgacc taggcgagcc
     5041 cgcagcctct aatgaaattg caatgggctc ttcagagtga acctcactat gaacctggcc
     5101 cgtccgccta tccgtatccg ccccttgtgg attgccttag ctctttctct gttagtccac
     5161 gtcttacttt tgggtcatcg ctggtttgag gcgcaggaac aggatcgtcg cctaagtaca
     5221 ccattaagcg ttgtcctagt aaatgctagt actaaatcgc cacctcaaaa cgcacagaag
     5281 cttgcccaag cagatctcga tgggggtggt ttgaccatta atcaagatgc cagcgcgatg
     5341 catcgcgcgc gtttgggcgc agatgcaaaa ttggaagcac ttgagaaacg tcaaaagcag
     5401 ttgctgtcta aattagaggc tgataaaaaa caggcttccg gtaaacgaag tggggaagag
     5461 gctaaacaga ttacagaaat gaatgctctt gaggctgagt tagcaaaacg tcttagcccc
     5521 gaggggcgat taccgcggcg ggcagtgtta agcgctagta gtacaaagac cgtggccttt
     5581 gcgtattact acgatgctat gcgccaaaag attgaggcct atggcaatac tttctttccg
     5641 agagtgcagg ggcgtcctat ctatggaagc ttagttttaa cagtcagtgt cgatgctgaa
     5701 ggctatattg ctacaaatag taagggacgc gaaggtattg aggtaagccg ttcatcagga
     5761 gttcgggagt tagaccgtca agcgattgcg attgtgcgag cggccgcacc atatggcccc
     5821 tttcctccag agatgcgtcg gcagctcgac attttggatt gggtctctac ttttgagttc
     5881 acacgcgaca ccaaagagca atctggaaac cgttttgaat taaagaatgg catgaatcaa
     5941 tgagtgctta tccatttctg ttgcgattag agcccgagct ttttaaagat agggatgtgt
     6001 atgctgtctt tggcaatccc attgcgcaca gtaaatcccc tttaattcat gagcaatttg
     6061 ctcgtaatag tggacaacgt atcagttata tccgtataca gcccgagctc gatcaattcg
     6121 ccaagatgct tgctgctttt tttcaaatgg gcggtaaggg cgccaatgta acagtccctt
     6181 tcaagctcga tgctttcgtc caatgtcagc aattaagtcc acgcgccaaa ttagcgggtg
     6241 ctgttaatac cttatggctt gagaatggtc aattgattgg tgacaatacg gatggaatcg
     6301 gcttggtccg tgatttgcag tcacagggta ttgggctaaa taacaaacgt gtattgatag
     6361 taggcgccgg tggcgccagt cgcggcatca ttggccctat tctgcaagaa gcgccaaata
     6421 gtctggtggt ggtaaatcgg accattcata aggccgaaga attagttgct acatttaaaa
     6481 atcttgccaa ttccttggcg gtggatttag aggcctggcc actcttaaaa ctagagcaag
     6541 ataatgtgcc tttgtttgat ttggttatta atgcaagcgc agccggcttg gaacagcaaa
     6601 gcccattgag cgatttggcc gcaaagcatg tattccagtc cagttgtttt gcctatgatt
     6661 tactttacgg caagatcacg cctttcatgc atcaggctct gcagaacggc tgtcgggtta
     6721 gtgatggtct tggaatgttg gtggagcaag cagcagaagc atttattctt tggcgcaagc
     6781 tgaatataga gtcgctagga acacgcnnct gcgaaaggct taggccccat gcggtggctg
     6841 acctatttct taaaatgcat tgcttgggga tttgtttcga tccaattgta ttttgtagtg
     6901 caaattacat tatggatctt tattaatcca agtagcaccg cttttcagag ggcagagcat
     6961 tggcgactat gtcatcttag tttcacttgc ccgattcaac atcgttgggt tccttatgca
     7021 caaattagca atgatctcaa gcgcgcaatt ttagtgagcg aggacgatat cttctttata
     7081 catccaggcg ttcgaattga ggatatgcaa aaagcttggg agagaaatca aaaagcaggc
     7141 agtaaggtcg tgcgcggcgg gtccacgatt actcaacaat tggctaagaa cttatttctc
     7201 tcctcagaga agaattactt ccgcaagact caagagctaa tcgttacggg attattggaa
     7261 cttattttat caaaacaacg aatcttagaa atctatatga actcggtcga gtggggcgag
     7321 ggtatttttg gagttggtgc tgctacccaa cattactttg tgagcactcc aaaaaatatt
     7381 acccgtgatc aagctgtctc tttggcatcc gccttgccgg caccaaaatg ctttgataag
     7441 agtaaatatt gcaaacgagg cagcattgac tttagtgctc gccaacaatt tatcttggag
     7501 ggtatggacc gaattgctct tcccaagcca aaatagaaaa aatccagcgg cttagtttct
     7561 gactggattt ttactttagt ttttcttggg cagtacgcag cgcattgcgg gtctcttgcg
     7621 caacccctct cgcagcgctc gcataatcgt cacctgagct ggcatacaaa attgcacgag
     7681 aagaattaat catcatcccg atacctggac gcttagaaat tgcaccagcc atcacagtag
     7741 cctcaatatc gccaccctgt gcaccaattc caggaattaa tagaggcata tcacccacaa
     7801 tcgatctaac tttggcaatt tcttggggat aggttgcacc aaccaccaga gcaatctgac
     7861 cagattgatc ccattctttt gcgagctggg ccactttttc aaagagagcc ccattattcc
     7921 caacatgaag gttttgcaga tcggagccac cggggttaga ggttctgcag agaatgatta
     7981 ctcccttgcc tgcatgttgc aaataaggct caatcgaatc cttgcccata tagggattga
     8041 ccgttactgc atcggcccca tatcgttcaa aggcctctag ggcatagtgc tccgcagtac
     8101 tgccaatatc tccacgtttg gcatccaaga taacggggat attggggtac ttggttttga
     8161 gatgagcgat tagcttttcg agttgggcct cggccccctg agaggcaaag taggcaaact
     8221 gtggtttaaa agcacaaacc aggtctgcgg tcgcatcggc aattgtctga caaaactcta
     8281 ggatggcttg aggcttacca gctaggcagg atggaaatcg cttggggtct gggtctagcc
     8341 ccaaacagag catgctgccc tgtgaagccc aagcagcctc aatctgatcc cgaaaggacc
     8401 cccccttttt tgtcacatgt aatgaactgt tcatagtgca taggataaac tagcgcattc
     8461 gtcgggagtt caccatgatc aacttgttcg tcctacaaaa tggccgtcta tcgcaagagc
     8521 aagtcgaaga tcgcaatgaa ctacttcagc accataatcc tatttggatt gacgtcatcg
     8581 acccggaaga ggaagaactc ctctggatca aagaagcttt tgcagttcaa ctgccagaac
     8641 tggatgattt aggtgatttg gaagcgtctg ctcgttactt tgaggcagac gatgggcatc
     8701 tgcatattcg cactgatttc ttgttagacg aagaagacac atcacgcaac gtgagggtgg
     8761 ctttcgtatt aaccaaccaa gttcttttct ccattcacga tgaagactta ccagtattta
     8821 gactcgttcg tcttcgtgcg cggttacgcc caggctctgt tagtaatgcc aaagatgttc
     8881 tcctggattt gtactcaacc gatgctgaat attcggcaga cgcgctcgag gaggtttatg
     8941 aaaatctaga gcaagcgggt aagcgcgtct tatctgaaac tattaccgat gccgatgccg
     9001 caggtgttct agagacgatt gcgactgagg aggataccaa tggccgcatt cgtcgtaacg
     9061 tgatggatac tcggcgcgcc ctctcatttc taatgcgcag caaattactc tcagatgagc
     9121 agcaagaaga ggcacgtcaa atcttacgcg atatcgattc cttagaaaac cacaccgcat
     9181 ttttgttcga taagatcaac tttttaatgg atgcaaccgt tggctttatt agtttgaacc
     9241 agagcaaaat tattaagatc ttctctgtgg tttcggtcgc cctgatgccg cccaccttgc
     9301 tcgctagcat ttggggtatg aactttgagt ttatgcccga gttaaaagag acctggggtt
     9361 atccggccgc catcatcacc atgattattt cagcgatgat tccattaggg tacttccgcc
     9421 ataaaggttg gcttaagtct aattaagagt caacagctca gtctgggcgg cttaggattt
     9481 gtattaattg gtctagagca taaatacagg cttgttcacg gatcgcctga cgatcgccat
     9541 tgaagtgctg agtgtttccg atcagccgcg caggattact tggcttctga attgcccaag
     9601 caaagcagac catgccaact ggtttgtttt cagtggcgcc acctggtcca gcaatgccgg
     9661 tgatggcgat acttgtttgg gcttcgctgt gtttcaacgc tccctgagcc atggctttgg
     9721 cgatctcttc actgacagca ccgtattgct caatttgtga gggcgatacc ccaatactct
     9781 cggtcttggc tgcgttacta taggtgacaa agccacgatc aaaccaacgg cttgatcctg
     9841 cttgagctgt caggtgggct gccaataagc cacctgtaca ggattctgct aaggcaatct
     9901 tgatggacga tttctccaaa agattggaaa gcgtttgtac aagtgaagca atagattgat
     9961 tcatcagaag agtctcatcc ccaacagaat aagaacgatt gtaaaaaaag ccgctaagag
    10021 atcgtctgcg atgattccaa aaccctgcca gataattgcc tgcgctgaat ttacatgagc
    10081 atttcttttg aaatatcggt caatgcgatt gatgggccag ggtttgatgg catccaagaa
    10141 acgaaataat gcaaaagcaa gaacttggat tgcaaatgac ccagacccaa tacaaagaag
    10201 aactaaccaa aacgcaataa tctcatccca cacaatgctg ccatgatcag gatagccaag
    10261 atctttgctg caaataccgc aagcccagca accatagact aagccaaaaa taattacgan
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag tgcaacagta cgatggatgg agcctagcat
    10441 ccaggcaaaa gatagtgtgg gagaaagact catgattgct gagggctgat tacttgaaat
    10501 gatcaaagga ttgcaagaga gtatttgtta gctctgcccc taaaagcttg ccgtcatgat
    10561 caagtagagt aatgctttgt gcatcctcct tactctcgac cagttggcca atgcgagtga
    10621 gcgtaattcc taggtcgttg gcaatttgtt gaatgtgaag acgatacttt tgatcagctg
    10681 taaaacaaag ctcatagtca tcaccacctg ccgctgcaca ttgccgacga atctgcgctt
    10741 cttgttcgtg taaaacattc gatattggta aggaatcaat ccagacctca gcatgaaggt
    10801 gagatgcttt cagaatatgc ttgagatcgc ctagcaagcc atcagagata tcaatccctg
    10861 cacttgcaat acctcgcaat gcaataccca agcctacgcg cggcattggt tcatgtagtc
    10921 gctgattaat catttttaaa tgggtggcac ttagattgag agtccattca ttgcgagcat
    10981 gggctagtgc taagcgcgca tcaccaattg tcccagatac ccaaatatcg ttattcacct
    11041 gtgcgccgct tcgctgaatg ctttgctgac gaggcacgct accaagcgca gtgatgctaa
    11101 ttgttaatgg tccagcgcag gtgtcgccac caatcaaggg acagttaaat tgattagcct
    11161 cttcaaacaa tcctcgcgca aatgtttgta tccaaatatc atccatactc ggtaatgcaa
    11221 gggagagtgt gaagcccagt gggtttgcac ccatagcagc caaatcagaa agattgaccg
    11281 ctagggattt cttccctaag cagtatgggt cagcatctgc aaagaagtgt cttcccgata
    11341 ccagcatatc gctagtgatt gcaatgagct ccccctcttt gggtttgagt aaggcgcaat
    11401 catcaccaat acctaatgca ggggtatttt caggattttt tttggccatt gcttcggcgc
    11461 ctgccttaaa gaccgattca atcaggacaa actctcccac cccctctgtg ggcaccttta
    11521 ttgtgttgga tattggctta gacatagact cattttaggc ggggtttggt atatctcgtt
    11581 ccgtgcgtga acggcgttag ctgggagtgc gctcaatacc cctttcgcag caatcctttg
    11641 ggaggaatag aatagacatt tgagcaaata tttaacaaaa atgatcattg ttaattgcaa
    11701 catttagagc ctcctaggcc ttaaaagcta tttcacaccc ataaaacccc tccacctctt
    11761 acctgcagat cactatgagt cagtctggaa acaatcccaa tcaaaataaa gaagagcaaa
    11821 ttaaggctct gcgccaagcc gccttgcaat accacgagtt tccggttcct ggaaagatag
    11881 cggttacgcc aaccaaacaa ttaaccaatc agcgggactt agccttggcc tacaccccag
    11941 gggttgccgc tgcttgtgaa gagatcaaaa aagatccttc tactgcattt cggtacaccg
    12001 ctcgaggaaa tttagtgggt gtagtaacca atggaaccgc tgtcctaggc ttgggcgata
    12061 ttggcccact agccagcaag ccggtgatgg aagggaaggc cgtcttgttt aagaagtttg
    12121 cgggtatcga tgtatttgat attgaggtca atgaaagcga tcctgagaag ttggtggaag
    12181 tgattgctgc tcttgagcct acctttggcg gaattaattt ggaggatatc aaggcgccag
    12241 attgcttctt ggtggagcgt aaattgcgcg agcgcatgaa gatcccggtc tttcatgatg
    12301 atcagcacgg cactgctatt gtggtggcag cagccattct caatggcttg aaggttgttg
    12361 gtaaagacat tggtaaggtc aagctggtca cctcaggcgc tggtgcggca gcgctagctt
    12421 gtttggagtt attggtggat ttaggtttac cgaaaagcaa tatttgggta accgatattg
    12481 tcggtgtggt ttatgagggt cgaaccgagt tgatggatcc aaaaaaatta ccgtttgcgc
    12541 aaaagaccga taaacgcaac ttaggtgaag cgattgaggg cgccgatgtt ttcttgggat
    12601 tgtccgctgg aggagttcta aagccagaaa tggtgaaacg tatggccgat aagccgctcg
    12661 tatttgcatt agccaatccc acccctgaaa tccttcccga agaggtgaaa tcagttcggc
    12721 cagatgcggt catggcaaca gggcggaccg attaccccaa tcaagtcaac aatgttttgt
    12781 gtttcccttt tatatttcgt ggcgctttgg atgtgggcgc taccaccatt acccgtgaga
    12841 tggaaattgc ggcggttacg gctatcgctg acttggcgcg agccgaacaa agcgatgtag
    12901 tagctgcggt ctatagtact gagaatttgg catttggccc tgattattta attcccaagc
    12961 cgtttgatcc ccgcctgatt accgtgatcg cgcccgccgt tgctaaggca gctatggatt
    13021 gtggggttgc tagtcgaccc attaaagact ttgcgcttta caaagatcag ttgcaacagt
    13081 ttgtgtatca ctcaggcacc atcatgaaac cgctctttgg cattgccaag aagattccgg
    13141 cagagcaaaa acggattgtt ttctgtgagg gtgagaatga gcgtgtcatg cgcgctgtgc
    13201 aaatcatgct tgatgaaaat ttagcaaatc cgatcttaat cggtcgcccc tctgtaattg
    13261 agcatcgcat tgagaaatac ggcctgcgca tgaagcctgg tgttgatatt gagattgtga
    13321 atcccgaaag cgattctcgg tttagggatt tttggcagac ctatttacag ctgacagagc
    13381 gtaagggggt aacggagtcc tttgccaaat tagaaatgcg tcgtcgtaat actttgattg
    13441 gttctattct tctaaaaaaa ggtcacgctg atggcatgat ttgtggaacg attagtaacg
    13501 tcggcaccca tctcaaatat atcgatgaag tcatcggcca tgaactgggg gcgagtgttt
    13561 atggggggat gtcggggcta atattgccca atcgccaagt cttcttggta gacacccatg
    13621 tcaatgttga tcccacagct tcacagttag cagaaattac tttgttggcg gctagcgaga
    13681 tgcggaagct gggcatgacc cccaaagttg cccttttatc ccattccaac tttggttcaa
    13741 gtgatgcgcc ttcggccctc aaaatgcgcg aggtcttggc aattttgcaa gcgactgcac
    13801 ccgatctgga agtcgatggt gaaatgcatg gcgattgcgc tcttgacccc gaaatccgcg
    13861 caggggcggt cacacagtcc agattgaagg gcgaggctaa tttattggtc ttacccaata
    13921 tcgatgccgc caacatctcg tataacttat tgaaaactgc tgctgggaac ggcgtagcaa
    13981 ttggcccact cttacttggt gtggcaaaac cgatccatat tcttaccccg gcagccactg
    14041 tgcgccgtat tgtgaatgtg acaaccttgg ccgtggttga ggctgcaagt caatctagag
    14101 ggttatttta agtaatagta agtagtcgct tacttttaat ttatttatat aaatcaatag
    14161 cttataaaat aagta
//
LOCUS       DDPQ01000110            6136 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5170,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000110 DDPQ01000000
VERSION     DDPQ01000110.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5170"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    4755..4793
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5883..5907
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1625 a         1617 c         1324 g         1506 t
ORIGIN      
        1 gggccgcata aggtgacatc cacacccgct gctgagaatc cagactccaa tgcagcctct
       61 aataaatatc cagagacgcg cgtgtcttta ccaatcaata cccgttgacg ctcatttgat
      121 ttggcgtggc gtatcaatac cttgccagcg gcataaccca agcgcaacat gaagtctgga
      181 acaatgggtg gaacaccaac ctctccacga atgccatcgg taccgaagta ttttttagtc
      241 attcttcatt ataaaacttg taagctcttg ctctcgcatg gcttgccaaa tctttatggc
      301 atccatggtc tcactcacat cgtgcgttcg cacaattttg gcaccacgct caaccgccat
      361 gatggcagca gcgatgctcg gaatcaggcg gtctccaact tctttatggg ttactttacc
      421 cagcatggat ttgcgtgaga tacctgctaa gaccggatag ccatgctctg taaacaatgc
      481 aaattgggct aagagttgat agttatgctc gaggctctta ccaaaaccaa atcccgggtc
      541 aatggtgatg cggttcgatg caatgccagc ctcttcaagc aatgcacagc gctccaataa
      601 aaaggtttgt acatcttgca agacattatc gtaatgagga tcaaattgca tggtttgtgg
      661 atcgcgttgc atatgcatga gaaccacgcc acagtttgat tgcttgacaa catccactgc
      721 tccctcttga cgaagcgccc agatatcgtt gacacagtcg acacctaaat caagggctgc
      781 tcgcatggtc tctggtttat aggtatcaat cgagagggct gcaccgcact ctttaagagc
      841 ctcaatcaca ggcaaaacgc gatcgagttc ttcttgtaag gcgaccggtt ctgcgccagg
      901 tcgtgtggac tcgccaccaa tatcaataat gtctacgcct gcagcaatca ttttctcggc
      961 ttgcttaagc cctgcatcgc gttgataaaa ctgcccacca tcggagaatg aatctggagt
     1021 ggcattcaaa atacccatca ccaaaggacg agtggtcttg gagagctcaa aaagaaaacg
     1081 cccgcagcgc catgccgcgg gcgatggatt aacctgcatc cttaggctgt tgcgggagcc
     1141 ccgcctgcag ccggtccagg tgtgccgcca ccagcggagt taccaaaggc agttgcagtc
     1201 actggcttgg gttccttagg aggacgacca gccatgatgt cattgatttg ctcggcatca
     1261 atggtctccc actctaagag agcagctacc atagcttcca ccttatcgcg gttctcttca
     1321 aggatcttct ttgctaaagc atactgacta tcaattaagg tacggatctc ataatccacc
     1381 ttttgttgag tcaactcaga aaccgttttg ttgttcatct tgccaaacat actgtcttgt
     1441 tcggtatcga catacaccat ggtgcctaag ctatcactca taccataacg ggtcaccatg
     1501 tcacgagcca ttttggtagc gcgctcaaag tcattagaag cgcccgtact catcgagctc
     1561 aagaacacct cttcggcagc gcggccacca aacaaaatag ccaactcctc caacatgcga
     1621 tctttgtaca aattaacacg atcaaactcg ggtaactgcc aggtgacgcc caaggccata
     1681 ccacggggca tgatcgtaac tttgtgaacc ggatctgcct tcggcaatat cttagccacc
     1741 accgcatgac ccgactcgtg atatgccgta ttgcgtcgct cctcttcgcg catcactgct
     1801 gacttgcgct ctgggcccat atagatcttg tctttagcat cctcaaagtc ttgcatatcg
     1861 accgcacgct tacttcggcg cgcagcaaat aaggcagact cgtttacgag gttcgcaagg
     1921 tcagcacccg agaaccctgg ggtacccctg gccaataccg caggatcaac gtccgtgcta
     1981 atcggcacct tacgcatgtg cacacgcagg atttgttcac gaccccgaat atctggcaag
     2041 ccaacatgaa cctgacgatc aaaacgaccg gggcgcaata aggcgcgatc caagacatca
     2101 gaacggttcg ttgcagcgat aacgatgaca ccgctgttgg tctcaaaacc atccatctcg
     2161 actagcattt gattgagggt ttgctcacgc tcatcattac cgccgcccat gccagcaccg
     2221 cggtgacgac caaccgcatc aatctcatca atgaagatga tgcatggtgc atgcttcttg
     2281 gcgttatcaa acatatcacg cacacgcgct gcacctacac ccacaaacat ctctacgaag
     2341 tctgaaccag agattgagaa gaacggcacc ttggcctcac cggcaatcgc ccgagctaat
     2401 aaagtcttac cagttcctgg ggggcccact aagagaacac cgtgcgggat acgaccaccg
     2461 agtttctgaa acttctgcgg atcttttaag aaatccacaa tctcgaagac ctcctctttc
     2521 gcttcatcgc atcctgcgac atcagcaaag gtcaccgagt tactgttctc atcaattaag
     2581 cgagctttgg atttaccaaa ggagaatgca ccgcccttac cgccaccttg catctggcgc
     2641 atcatgaaga accaaaaacc gatgatgagc aatgtgggtc cgaggtaata aagagctgag
     2701 accaaaagat ttggttcgtc ttcggtctta ccagtaacct gcacaccgta tttcatgaga
     2761 tcaccgacca tccagatatc gcctggagag atgagagaat atttattacc gtctgctggg
     2821 gtaatttgca aagtacggcc ttgcacatct acgcgcttga tcttgccact tttggcgtca
     2881 tccataaatt gggaatacgt gacctgatct tgtgtgcgtg gtttatcaaa ctgcttaaag
     2941 acagtaaaca aaaccaagcc aacgatgagc caaacaccca ctttttggaa caaattgtta
     3001 ttcaaattac acctttgccg gattgatccg ggagttatca gggccaagtc ttaaatgcct
     3061 accctatgta tttgattcta ctacctacaa aaatcaaggg ttgaaggaca aatttatcct
     3121 aaaaaggctg ggtttactat gcaaaaagac ttattttggg gtcttctgtc tgttttaccc
     3181 tacttcaggt aacgccctag cagaaaggtt tcagaggact tgtcgcggga ggccttgggt
     3241 tttctggggg ctaccaccct aaatacccct ttaaaagtct caactatttg gctgtagccg
     3301 cttccatgaa agcacttaat taaaagggcc ccatcgggct ttaaatggct tttggcgaac
     3361 tccaaggcaa gttctgccaa gagtgccatt cgagcagaat cagcaacccc aaccccagat
     3421 agattgggtg ccatatctga gagcacgaga tcaactttgc cattagcttc tgggggcaag
     3481 gctgcttcaa gcgctctaag tccctcgtcc tctctaaagt cgccttcaat aaagtgtacg
     3541 tctgcaatcg ggtccattgg caaaagatcg attgcaatga tgcaaccatc tggcttgcca
     3601 gcgttctcag ggcttggatt acggcgcagt tccacgagac gattacgcgc atactggctc
     3661 caactccctg gcgtactacc tagatcaata atggtcattc ctgctttgat gaggtgatct
     3721 tgtgcatcaa tctcgcttag tttgtaagca gcacgagctc gatagccctc cttctgtgcc
     3781 aacttcacat aggggtcatt tagatggtcg tgtaaccaat ttttattgaa cttgttcttt
     3841 gccacgatca ttattgtcgc tgttttctaa gcaggcttcg ataatcctct atgatctcaa
     3901 gatgactgca ctgaccttaa gccccgccca acgcaaagca catcgtgcga aagcgcatcc
     3961 tcttaaaccc gtgatcatga tcggcaatga tgggttcacg cccgccgttg ccaaagaaac
     4021 agagcttgct ctgaagagcc atgggctgat taagatccga gttctcggtg atgatcgcga
     4081 tgcccgagag caaatgttcc tcgagatctg tgaccagctc aatgcggcac cgattcagca
     4141 tattggcaaa ctgctcgtag tttggcgtcc ccaaaaatcc aaagaggaga aattagctcc
     4201 cagcaagaag gtggcgcgcg cttataaagt agctcgagtt gccaagaagg ctgccggctc
     4261 acgcattggg atgaagggaa cgaaacacgc gcctgcagac aaaccaaaac gtcgcaaggt
     4321 acgtcaagcc agtcccaaga aagccgctct cagctagctt gatttgcact gccaagtgaa
     4381 cgacttaggt accaaactaa gtagaccccc aagaggcttt gcaccaaaaa tattgcgctc
     4441 gatactccat gcaaggtaga gaacaaaccg gctaacggtg aataccgcac gggcattcct
     4501 tgcaataagg cttcctcacg caaactctcc atccaaggca tgagtacaaa gctaccgact
     4561 gccgcacaaa tcaacatgac tagcaagatc caccgaattg ctcgatagcc ttgcagcccg
     4621 cgactcacga gtaagtttgc gaggaccagt agaccgacac acacgactaa gctgatatag
     4681 gcctcgacct taaagatcgc accggcaacc ataccggcca cctgtcgatc acttaaggta
     4741 gcaaataagg tgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaataga
     4801 aataatcgtt gggcaatttg atgttggctg gaactgggat ataacatatc aattactatg
     4861 aaattaatgt ggccaactta gccaacacct catccatagt ttgagttgaa acactttcta
     4921 atttttttgc ttttcgtgcc gccaggtcca acacccttgg ctgatcacaa cgagccacac
     4981 cagctgtttt tgtacccacc aggtcaacag taaaaccagc cactcttgca aaattgccac
     5041 ctgttgtaat aggtactact attggggtct tagttaactt gttaaatggt cctggagaaa
     5101 caaccagcac gggacgaaac cctttttgct catagccaga acttggatct aaggagacta
     5161 aataaatatc accccgattc attacaaaat ctcattacca atcggggtta gctcaagcca
     5221 ctgcttatcc tctttcggca tttttgcatt agggtcgcac tttgccaata aatcagctaa
     5281 ctgatagctg ggctttgaga ttggcttcac caccaatcga ccatttgata aggcaacgtc
     5341 aacgatagat ccagaggaga tcttaagttc ctccaaaaat cccggtgata cagccatcat
     5401 cacagaaccg cctacttttc ttaaatgtgt ttgcatgact agctcctaat aggttatata
     5461 aaaatataac atactttgca ctacagtgca attatataaa aatataactt ataaggatta
     5521 ggttcttaga tataacgaac cgagaggatc tcaacctcgc gcgcacccgc tggggcttgc
     5581 acgctgacca cgtcgccctc ttccttaccg atcaatgccc gtgcaatggg agagctaatc
     5641 gagatcttat tggcagcaat atcggcttca tcgtcgccca caatctggta gctcattttt
     5701 ttaccatcat ccagatcttc taaatcaacg gtggcaccaa agaccacgcg accttctgcc
     5761 tcaagaagcg agggatctat gatctgcgca cccgatagtt tggtctccag ctctttgatg
     5821 cggccttcga taaacccttg cttctcctta gcggcatcgt actcagcatt ctcagaaaga
     5881 tcnnnnnnnn nnnnnnnnnn nnnnnnntgg agctcctctt tcaggagctc agcaccacgc
     5941 tttgtaatcg gaattgtgtt tgcactcata ccgctcaatc cttaaatcgt taattctgga
     6001 ttttaagcta gctaagcgca atatgtaaat tttgtaagga ataaacctct aaagagtcct
     6061 tggcgcgcat tccctccatc accgcacggg ctgcactaat ggtggtgtag taggttacgc
     6121 cattggcttg agcggt
//
LOCUS       DDPQ01000111            6205 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2887,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000111 DDPQ01000000
VERSION     DDPQ01000111.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2887"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    746..779
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1681..1715
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3628..3745
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1537 a         1429 c         1526 g         1526 t
ORIGIN      
        1 gtgctcgcct gctttgtaca tgcccccatt ggccttgcca atatcttgcg agagaacatc
       61 caaaacgctt ttctgaaccg tgccttacca ctcttgctgg cagccctaat actcatacta
      121 ggaaccatgg cggtaattgg tgtggtctgg ggacagccat gagccaaaaa tctacgcgcc
      181 aacttgattt tcggggaagg gctcactggt cttactttgc atactatttg catcgtgtat
      241 cgggtctact gttggcctgc tttctgcccc tgcatttttt cttactctcg cgttctttaa
      301 agggcgaagc tgagctggca agttacctca aactgaccga cttccccatc ttcaagtttg
      361 gggaatgggc tttggttact ctcttagcat tgcacttaat cggcgggaca cgcttgctcg
      421 taattgagtt tggtgtttgg aagggcttac ggaaagcatg gatccaaatg gcagttcttg
      481 gatcgctcgt atgtggcggc ttattcttaa tcttgtcacg ctagatcatc ccaggactta
      541 cttcattagg atcggatatg gaactaaatc tgcaacacgt tgaggacgca tgtaaggagt
      601 tgtatatccg cgcacttaaa ttacttcccg atgatgtgaa acaggggatt aatcgactga
      661 agagcaccga aaccgacgca cgtgcccaag tggtgctagg caccatggtg accaatattg
      721 cggtagccga gcgtgaagat aatttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      781 attcgcagag ggtgtgaacg cgccaccaca gaatacccac ttcggtcctc cgtagtacat
      841 cccattacac gtaagaacaa tcacacctcg agtggtattg agatgcctgc aatccatatt
      901 gattttatgg acaaggccga tacgattgag attgagatga tcccaaaagg tagtggctcg
      961 gagaacaact ccttcttaaa gatggccatc ccagctgaag gaatcttagg ggtcaaggcc
     1021 tttgtgatcg aaagtgttgt gaacgccggt ggtaaaacct gcccaccgac aatcgtggga
     1081 gttggcgtgg ggggcacctc ggatcaatgc gtcgccttag ccaaacgtgc tgccacccga
     1141 gcgctgggaa cacactgcag tgatgaagag ggtgccaaat tggaagtgga actgagcact
     1201 gccgttaatc aattggggat tggtcctcaa gggcttggtg gtgatggcac cgcttttgca
     1261 gtccatgtag aactcgctgc tacacatatc accatgaacc ccgttgctgt aaacatgcag
     1321 tgtcattcag cgcgacgtgc gcgggttagc atcacaccac agggtgtggc atacggattt
     1381 taggacgatc atggcccatt acagcttacc cacccctgtt agcgaagccg atattcggaa
     1441 gctcaagatc aatgacaccg taaccttgca acaaacactt tttgggattc gggatgccac
     1501 ccagatccat ttgtttgatc ggggtcgtaa aacgaagttt gatcttcgcg gtcatgccgt
     1561 gattcatacg gcgcccaacg tgcgtaaagt gcctgtcagt gcagagtttc ccgcagggta
     1621 tcagcccatt tgtattggta ccaccacctc ggatcgcatg gaacgattta ctgaacctct
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcatt tgcaaaacat ggtggggtct
     1741 acttagcaat cattggtggc acagccgctt tagagaccac ctggattgat cagattgaag
     1801 atgtggatct cgatgatctc aatccagagt cactctggaa gttcaagatt aatcagtttg
     1861 gcccactctt ggtagcaatg gatagccacg gtggcagtat ttatcaagac gtgaaagccg
     1921 atgtggcgat caataaagca tcggtactaa aaagtttagg gattgtgtaa ccatgacaag
     1981 cattcaaaca ctgcaaaccg atatcttggt cttaggctct ggcggtgcag ggctctttgc
     2041 agccttgcat gcacatcaag ccaatcctga tctacacatc accattgccg taaaaggact
     2101 tcttggtaaa tgcggatgca cgcgcatggt acagggtggc tataacgtag ccttggcaga
     2161 aggagattcg gttgagcgtc atttcatgga tacgattgaa gggggcaagt ggttatcgga
     2221 tcaagagtta gcctggaccc tggtcaacaa atcggttgag cgtattcgtg agctagagaa
     2281 tgagctcgga tgcttctttg atcgcaaccc agatgggacc attcatcaaa aggcatttgc
     2341 aggacaaacc tttgatcgca ccgtacataa gggtgacctc accgggattg agattattaa
     2401 tcgcttagcc gagcaagtat gggcgagagg gattcatcgc ctggaagaac accgcgccat
     2461 tgagttaata aagagtcagg atggcaaagc acttgctggg gtcttaatgc tcaatatgcg
     2521 taccggtgag tttgtgatgg tgcgcgcgaa ggctgtatta cttggcaccg gtggcgggcc
     2581 cacgatgtat aagtaccata ccccctcggg tgataaaagt tgtgacggct tggccatggc
     2641 cttgcgagca ggcttaacac tgcgcgatat ggagatggtg cagtttcatc caaccggaat
     2701 gctcgctggt ccagataccc gtatgaccgg cacagttctc gaagagggct tacgtggtgc
     2761 gggtggttat ctactcaatg gcaataaaga acgcttcatg ggccattatg accctcgcaa
     2821 tgaacgtgca acccgagata ttgtctctcg atcaattatt tatgagatgc gtgcgggacg
     2881 cgtgacccca aatggtggcg tctacatcca aatgcatcac cttggtcctg ataatgtgcg
     2941 caaattattt aaaggcatgg tggaacgctg tgctgattct ggatttgatc tagcaaatga
     3001 tcttgtggaa gtggtaccga cagcacatta catgatgggg ggtgtggtgt ttaaagctga
     3061 taccagtacc gatctgccag gtctctttgc tgctggtgaa gataccggcg gtgtgcatgg
     3121 agctaaccgt ctcggtggta acggagtggc caactccacg gtctttggtg gcatcgccgg
     3181 cgacactatg gcgcaatatg tcatgggcaa gaaccatctt gattacgatc ttgaagccat
     3241 cgaacaaagc ctgcttgagc atcaagagcc gctgaacaaa gcaccgggtg atattgagtt
     3301 catccgcgat gagctagccg attgcatgtg ggatcaagtt ggtatcttgc gtaatcaagc
     3361 cgatcttgaa gcagcacgga tacgtctaaa caaactcgat gacatattgc atacgatggg
     3421 ggtgggtgag accaaccgag catttaatct gacctggcaa gattggatga acctgcgcaa
     3481 tctcatcctt gtaagtaaga ccgtggtcga ggcagcctta gcacgagaga actcgcgcgg
     3541 cgctcactac cgtgaagact acccagaaga gggtgaccta gaaacctcct tcttcactgc
     3601 tgtacacatg aaggatgagc aactcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnattga ttgctgctca gttaaaactg agtactgcag
     3781 agtgtgtaat cagttagcag tcaggttacg tgccttaata actttctccc acttaataga
     3841 ctcagaagca atttgtgcct cgagctcttt ttgggtattc aggacagggg ttaagctatt
     3901 gagctctaaa ctcttttgca tcgcctcaga ctttactgcc ttaatcgcag cagcgtagat
     3961 cttgtcggta atcaccttgg gggtgttgga tggcgccatc aaagcaaacc aaccaacgtt
     4021 ctcaaagcca gggatagaag cttctgccac tgttggaata ttaggaagag ccgctaaacg
     4081 cttagcgctg gtcactgcaa gcgccttgat ttgtccactc ttaataaagc ccgtagaggc
     4141 aggcacgttg caggcacaga aatcaatttg tcctgcaatt aaatcactga gagccgccgc
     4201 ctcacctttg taaggaatat gggttgcatt aataccagct gccgtaatga agttctcacc
     4261 agcaaggtgc acctgactac caataccagc agaaccaaag ttgaggttct tggtcttagc
     4321 aagcgcaatt aaatcattga gattgttaac tgctagcttt ggattaacag aaataatcat
     4381 tggaccacta accaaggtag agataggagc aaagtctttg gcgtaattaa tcgacatctt
     4441 cttatacata tgtggattaa cggtcaacat gctacctgag gcgaggagta gggtgtagcc
     4501 atcagcaggc gctctggcta cagcctcagc accaatattg ccaccggcac cgccacggtt
     4561 ttcaactact acgttctgac ccaagatggg agccatttgc tgcgccagga tacggccaat
     4621 aatgtcagtg gtaccgcctg ctgcaaatgg aatcactaat ttgatcggct tatcaggcca
     4681 actttgtgct tgcgcaggac tcatgaagcc catggtggcg attgctgcga tgccaagaac
     4741 ttttgaaata gttcgataaa agcgctggtt cattctatgt ctccttgtag atccttgtgg
     4801 atttttatat tacgaaactg ttgatactca tttaaccata attttctaga aaaccctatt
     4861 gcacgagatc tatgtcatct atatgatatg gatatgtcag ttattttcat ctccgagttc
     4921 atctcccgtc aagccctaga aacccttaaa caatctcatc aggtgcgcta tgagcctgag
     4981 tcctactctg accccagcaa attagcctca gggttaatca atgccgatgc ctggatcgtg
     5041 cgcaatctca ccaaagtaag tgctgatctg gtccaaggtg ctaagcaact gaaagtagtg
     5101 gggcgcctag gagttggttt ggagaacatt gatctacccg cctgtgcaca agccaatatc
     5161 aaggtcattc cagccaccgg tgcgaacgcc gactcggtcg ccgagtatgt gttgggtaca
     5221 tcgatggcac tcatgcgtgc ttatgcaccc gctagtcttg agaccctctc aggcacttgg
     5281 ccaagaccca aatactccaa atgccatgag atcgcaggta acaccttagg gattgtgggc
     5341 tttggcagca ttggtagggt ggttgcacaa aaagccttag ccctaggatt gcattgcatc
     5401 gcctatgatc cgatgctaaa agataaacaa gtggcactcc aaaacggcag cgttcattta
     5461 tatcccttag cagaggtatt agctaaaagc caaattgtga gtttgcacct accgctatta
     5521 cccgagacca aaggattgtt ctcggcaact ctgctcgata cgatgcaagc tggcgcctat
     5581 ctcattaata ctgcacgcgg tggcattgtc gatgagcagg cattagcaga gcgcctgcga
     5641 tctgggcgtt tagggggagc agcgcttgat gtctttgcaa gcgaacctgc caaagaccta
     5701 tcgcatttca aggatgtccc caatttaata ctgaccccgc acattgcggg agttactgaa
     5761 gaaagtaatg aacgtgtgag caatatgatt gctcgcgaag tgaatgcatt tttggagaac
     5821 cattcgtgag tcattcattt aatgagatgg tgcagctcgc ctatcaaggt ttacgtgctg
     5881 ctgatattgc ccatgatgcc gccgccgata ccgcactctt tttggctctt gcagaagcgg
     5941 atggtctagc atcacacggc ctagcccgcg ttgcccaata cgctggccat gccaagcatg
     6001 gtcgtgtgaa tacccaagcc aaagcgaagg tgcatgctta taaagcagcc tctgccgtag
     6061 tggatggtca agatggtttg gcattccccg ccattaaaac agctaccgat ctatccgtac
     6121 gcttagctaa ctcacaaggt gtaggagtag ttgctgttaa gaacacccat cactttggag
     6181 ttgccggaca tttcaccgaa gcagc
//
LOCUS       DDPQ01000112            3906 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11356,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000112 DDPQ01000000
VERSION     DDPQ01000112.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11356"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    706..758
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1541..1565
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3494..3607
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          923 a          910 c          935 g          946 t
ORIGIN      
        1 caaaactgcc ctggcgtggt tacgcgaagg taagcgcatg gccatcgcga cggtggttca
       61 aacctggggc tcctcgccgc ggcctgttgg ttcatggctt gcgattcggg aggatggaca
      121 agtggttggc tcagtatctg gtggatgcgt ggaggatgat ttaatccggc gggtacagac
      181 tgagatcttg acgcgtaata ctcctgaact cgtggtgtat ggcgttagca aagaggaagc
      241 ggctcgcttt ggtttgccct gtggtggcac cttacgctta ttggttgagc ccagaccaga
      301 gcaaggtatt ttagacagca ttctcgagaa gattgaaagc catcagatca tattgcgacg
      361 ggttaatttg agttcaggta aatccaccct tcacctagga actagagaag atgggttctc
      421 gctaagcgag tctgagatgc aaacctccta cggtccccgt tggcgtatgt tcgtaattgg
      481 cgcaggacaa ctctctttat atgttgctaa ttttgctcta gcggcagact ttgatgtagt
      541 ggtcattgac ccccgtgagg aatacaccga aggtcttagc caagaaggta tccagttcat
      601 caaaggcatg cccgatgatg tgatgcaaga attagggatt gactcccaca ctgcgattgt
      661 ggctctcacg cacgacccca agattgatga tcttgccctg atcgannnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gttcaattta tcgaccgagc
      781 aactcgatcg tttgcatggg cctgtgggtt tatccattgg cgccctaacg cctccagaga
      841 ttgcggtctc ggtaatggcc gaagtgattg cggtcaagta cggcaagcaa aactaaggtc
      901 tgattgccgg gctttcaatc cgcataccat ttgcacgacg cattagataa agctcgattt
      961 gggcaagctc cacagagcca taaggatatt gttctgcccg cactcccgac atgcaattac
     1021 gtaatctacg ctgtaaggag ccgacgttct gccattccaa acgatagatc ggatacccag
     1081 taggatgcgc ctggggaata acgctacccg ccagctttaa gcctgctctg tcatcgtggc
     1141 actgcgcaca cgatagattg agctgcccca ttctctgaaa ataaagagtt ttaccttttt
     1201 gaaaatcagc atctattgca gaatctttcg aagctgtaat tggcaggcct ttggattgat
     1261 aggcaatcag agtacttagc gctagtaatg gcttagactc atacgcaaaa gcgggtgatt
     1321 gttggcgagt ggtacggcat tgattaattt gcccttccag attgagtaat ttaccttgaa
     1381 cccgttttgg aaaggtagcg gagacacctt tcatggattg cttcacatca ctatgacaat
     1441 caccgcaggc tttactactg ggattattac gatcgatcca aagagcctcg ccgtccatga
     1501 tccaaaacat ggctggattt aagctcatat cattttgcat nnnnnnnnnn nnnnnnnnnn
     1561 nnnnngcttg tcagcaataa acccaataag gcacattgcc ctagcaatct cataaaacag
     1621 taatggcctg tttcacgatc gtgaggtaat cgttatcgcc cgtccactta aactcgagag
     1681 ttccggattg gtctgctttg gcatagaaaa taaagagtgg gttcgagccg acgcccggat
     1741 gaaggtcagc cttaaatact tctttgccgt tataggtaca aacaaactcc cgaataatgt
     1801 cgcggggcac gcgaacaccc tgctcggtat gccgaaagcc cgactccatc tcatgctgca
     1861 caatggcgcg aattgaaaat acctcacccc gttttgctgt tttgggaaga gtaacgactg
     1921 ctcgagagga acgactcata tcatctccgt acaggcagag agggttacca ccgtatcaac
     1981 gctggtgcgc caaaatgagc catcgctcat ttgtgccaaa gcccatacct tttgggagtc
     2041 agccaaacga atccgcgtgc ttgcttccgc cttacccgat aggggtgtga aataacaact
     2101 caccacgttt ggtaatggat tagcctcggc gatgatgtgt atggctttaa cgtaatcgtt
     2161 ttgcgtcatt gggctttgta cagaaaactt aatcgctact aaattgccgt tctcgacaag
     2221 gggtggtagc tcaaaataga tacgccccgt atttacagga cgattgccga caatcgcttt
     2281 catcgcttgc attgcatcgc ttggggtcgc gcttgtgctc tgccatgggc ttaatgtaag
     2341 cgcagtcaat gaggtaataa atacacgacg ctggttcatg gtgttttgat ggttaacaaa
     2401 taggcaatga catcttcaat ttcttgggca ctcaatacgg tcttaccagc aaattgaggg
     2461 gcaacgcgct ggagatgatc gattctaaag taggaaggca tgatcgtgtt ggcactggtt
     2521 ttagcagggt ctactaagcg agcgcgcagt tgtgcggccg atagattagc aacgctgagt
     2581 tttaactctg gggctaagtt accctgaaaa cgttcttctg gaaacgggcc ggcgtggcac
     2641 aggatgcata ggccggtctg ccggctggtc acaagtgtgc ggcccttgat ggggtctcca
     2701 ggtttaccgc ttaatgaatt tgggatggca tcgccatccc aaatgatttg cgcctgagag
     2761 cttgtgggaa gcgctagcag cgcactaatc gctagcgcaa tcccaaaccg cataccttag
     2821 acgagcgtaa taccgctgtt ctttagaggt actgtgcgga tccgcttacc cgttgcccgg
     2881 tagaaggcgt ttaagacagc aggtgccgct actgaaatag taggttcacc aataccgccc
     2941 cactccttgc caccaccctg aataatgatg gtctctactt taggcatttg ttttaagcga
     3001 atcgatccaa aggtatcaaa gttggtctgc tcaatccgac cattcttgac cgtgcactct
     3061 tcctcgaata aggcagataa gccatacaca aacgagccgg agacttgacg ctcaatttgg
     3121 gcaggattaa ctgcgtatcc aggatcggtt gctgcaacga tgcgatgaat cttgaccttg
     3181 ttgccatcgg tgaccgatag ctcacaggca gcagctacga aactaccaaa ggatttttgc
     3241 tgtgcaatcc cacggaatac cccggcgggt gctggtttgg tccaaccaat ccgatcggct
     3301 actgcattaa gcaccgctag attacgtgga tagttcgcag agtacttgcg acggaactcg
     3361 actggatcga ctccagcgta ttctgccaac tcatccataa agcattccat gaacaccgca
     3421 ttttggttaa tgttcacgcc ccgccaaaat cctggaggta cgtgcgtatt gcgcatcgcg
     3481 tgatcgatca tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnngca accactgcag ggcgcacgga ggtcaagatc gattgacccg ataaacgcat
     3661 atgaacaccc gtcaggtttt tcttgctatc aaagctagcg ctcattttgc acatcatgat
     3721 ggggtgatag cgaccttggg tcatatcttc ttcacgagtc caaatcaact tgattggtgt
     3781 gcctggcatt tgcttagcaa tattgaccac ctgggtggtg tagtcctgga aagcgccacg
     3841 acgaccaaag ccaccgccta gatttacctt aatgacatca cacttatcag cggttaagcc
     3901 cgaggc
//
LOCUS       DDPQ01000113            5203 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_59161,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000113 DDPQ01000000
VERSION     DDPQ01000113.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_59161"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    494..538
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4470..4551
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4785..4850
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1211 a         1220 c         1246 g         1333 t
ORIGIN      
        1 tttcctctgg aatctaagat ctctcttcca ataatgtcaa cgatggcact catgtaatct
       61 cctcaataaa tggtcttaga actcgatcgc actacgagta atttgcaaag cgatcttcta
      121 aaaataggtt gttctgttta acactagcat caatggcctt taaggatgcc aagagctctt
      181 tcagatgttc taggggtaca gcgttcggtc cgtccgatag tgctttagca ggattggggt
      241 gggtctccat aaataagcca ctaataccaa ccgctaccgc tgctctagca agaacgggta
      301 caaactcgcg ttgtccaccg ctactacttc cttggccgcc tggtaattgc accgagtggg
      361 tagcatcaaa aaccacgggg gcttttgtgc tgcgcatgat cgcgagactt ctcatatccg
      421 agaccaagtt gttgtaccca aaactagcgc cgcgctcgca gaccataaat tgatcgggaa
      481 gatttttctc ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
      541 cttcttgatg ttcacaggct taccactttg cgctaccgct gtaatgaaat cagtttggcg
      601 acacagaaat gcgggggttt gcagaacatc gaccaccgct gctaccgagg agacatcgct
      661 aatgtcgtgc acatcggtga ggattggcac cccaatttgt ttctttacct tcgccaagat
      721 ctctaaacct ttatccatcc ctaagccgcg aaaggatgag cccgacgagc gattggcttt
      781 gtcaaaggat gatttataaa taaatggaat atccagttct gcgcagatcg tcttaatctg
      841 ccctgcggta tcgatcgaga actgttctga ctcgatcaca catggtcctg caatcagaaa
      901 gaatggttga tctaagccaa tatcaaagcc gcagagtttt ttaaacatga tggatgcgtg
      961 ttggattaag cagcaactgc ggttttggat tgttgacgcg cgagtgctgc gcggatgaat
     1021 gccaagaaca gggggtgtcc atcacgcggt gtggaagtga actcggggtg gaactgcacc
     1081 ccaaagaacc aaggatgcat gctctgcggt aactccatca tctcaggaag atcctcggtt
     1141 ggagtacgtg ctgagatgat catgcctgcc ttctctaatt taggcgcgta gatattgttg
     1201 acctcatagc gatggcggtg acgttcgttc acttcattgc catagatgct atgcgctaag
     1261 gtatttgctt tgacggggca acgctgcgat cctaagcgca tggtgccgcc tagatcagaa
     1321 tcggctgccc gcttctcaat cttgccttcg cgatcaagcc attcggtgat aagcgcaacc
     1381 acgggattgg gggtctctgg atcaaactcg gtactgttcg ctgcctctat cttagctacg
     1441 tggcgggcaa actcaatcac agcgagttgc atccctaagc aaataccgag ataaggaact
     1501 gcgttctcac gcgcgtattg aattgctttg atcttgccct cggtgccacg cttaccaaag
     1561 cctccgggta ccaagatggc attgagatca tgcaagcact cgagattacc ttgctctaag
     1621 cgctcggagt cgatataccg aatattgatc ttggtgtgat tatgaatacc tgcatgccgt
     1681 aaggcttcga ttaatgattt ataggactca gtaaggtcca catacttacc caccatgcca
     1741 atggtgacct cgtgctgtgg gttctccatc tcgtagacca agtgggtcca catcgcaaga
     1801 tcagcaggct tggcttggat gttgagctct ttgcagatca actcatccat gccctgtgct
     1861 tgcaacatct ctggaatctt ataaatggtg tcgacatccc agaccgagat cactgcttct
     1921 tcacgcacat tggagaagag cgagatcttg gcgcgttcat cttctggaat gcgtcgatct
     1981 gcacgacaga gtaatacggt tggcaagata ccaatctcac gtagcttttg taccgagtgt
     2041 tgggttggtt tcgttttcag ctccccagca ctttgtatcc atggcacaag agttaaatgc
     2101 acaaaggcac agtcgccttt tggcagacgc aagctcattt ggcgtgcagc ttctagaaat
     2161 gggagtgact caatatcgcc aacggtgcca ccgatctcac agattgcaac atcagcttta
     2221 ccatcgtgac tagcctttgc accccgctcc acaaaagctt ggatctcatt ggtaatgtgt
     2281 ggaatgacct gcacggtttt acccagatac tcaccgcgtc gttccttccg aatcaccgac
     2341 tcatagatct gaccggtggt gaagttattg ctcttgcgca tcttggcgct cacaaaacgt
     2401 tcgtagtgtc cgagatcgag atcggtctcg gcgccgtctt cggtcacaaa gacctcgcca
     2461 tgctgaaacg gactcatggt tccagggtct acattgatgt aggggtctaa ttttaggagg
     2521 gtgactttca ggccgcggga ttcaagaatc gcggcaagcg aggcggctgc aattcctttc
     2581 ccgagggaag aaactacacc accagtgaca aaaacgtatt tggtcatcgc ttaacgtctg
     2641 ttggtaatac gcaattatag cgatagcggc gcatctgtct aaaaaaagat gattcgggct
     2701 tgccctaggt agaatcaaag gatgcgattt ctagccgcct tactgatcac cctactaacc
     2761 ctcttttacc tgttccctac agcggttgcg gtttatcgta agcgcgccaa tacgggggcg
     2821 atctttgccc taaacctctt tttaggttgg tctttgctgg gttgggtagt tgccttggtt
     2881 tgggccttaa agcacgaaga aatcctctaa atgcacccgg tttaggggta ttttggctgg
     2941 attgcctgtc agtaaagttt tacaatatag cctactcttt taggctcatt tctgatcagg
     3001 aaacccatgt tagacgcata tcaaacccat gttgctgaac gtgccgcaca aggcatcccc
     3061 gctcttccgc ttaccaaaga ccaaactact gaactcctta aattactact taatcctccc
     3121 aagggtttag aagcagaact cgtagaactc attagttata gagtacctgc tggtgttgat
     3181 gaggcggcca aggtcaaggc agagtttttg gattctgttg ccaaagggaa gattgcttct
     3241 ccattgatct ccaaggtcaa ggcaacggag ttactgggta cgatgctcgg tggttacaac
     3301 atcaaaccgc tggttgaact tttagaagac aaagactgtg gcgccgcagc tacggaggca
     3361 ttgaaaaaga ccttattgat gtttgattac ttcaatgatg tcaatgactt agcccacaaa
     3421 ggtaatgcaa atgccaaggc tgtaatccag agttgggcca atgccgaatg gtttactagt
     3481 cgccccgctg tcccagaatc catgaccctg acggtcttta aggtcaccgg cgagaccaat
     3541 actgatgatc tatcccctgc acccgatgcg tggagccgtc ccgatattcc cctgcatgcc
     3601 accatcatgc tcaagaaccc ccgtccgggc attgagcctg atgaagcggg tgtgcgcggt
     3661 cccatgaaac aaattgaggc cttgaaacaa aaaggtcatc agatcgccta tgtgggtgat
     3721 gtagtgggta ccggttcttc tcgcaagtca gcgaccaact cggtcttgtg gtggactggc
     3781 caagatattc cctttgttcc aaataagcgc tttggtggag tctgcttggg tagcaagatt
     3841 gcaccaatct tctttaacac catggaagat gcgggtgctc tgccaattga gcttgatgtc
     3901 aatcaaatga acatgggcga tgtgattgag ttgcgccctt acgagggcaa ggtttataaa
     3961 aatggtgcgg tgattaccga cttcgcattg aagtccccag tgattttgga tgaagtacgt
     4021 gctggtggtc gtattcctct catcgtgggt cgtggtctaa ctgcgaaagc gcgtgcgtct
     4081 ttaggtctgc ctgcatccac cgagttccgt gttcccatta gtcctcctaa taataagaag
     4141 ggctttagct tggcacagaa gatggtcggt cgtgcctgtg gattaccaga gggtcaaggt
     4201 gttcgtcctg gtacctattg cgagccccat atgaccacgg tcggctcaca agataccacc
     4261 ggtccgatga cccgtgatga gttgaaagat cttgcgtgtt tagggttctc ggcagatctc
     4321 gtgatgcaat ccttttgcca tacctcggcc tatcccaagc ccgttgatat tcgcacgcac
     4381 catgagttgc cagcatttat gaccaatcgc ggtggcgttg ctctacgtcc gggtgatggt
     4441 gtgatccaca gttggttaaa tcgtttattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtctcgtt
     4561 gcctttgcag ccgcaaccgg tgtgatgccc ttggatatgc cagagtcagt attgattcgg
     4621 tttaagggca agatgcagcc cggtattacc ttgcgtgatt tagtcaacgc aataccgctc
     4681 tatgccatta agcgcggtct tctcaccgtt gagaaaaaag gtaagaagaa tgtgttctca
     4741 ggtcggatcc tcgagattga aggattgcct gatctcaagg tggannnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccatcatcg
     4861 agtacatgcg ttctaacatc accttgatga agtggatgat tgcgaatggt tatgaagata
     4921 agcgcaccct cacccgtcgt attaaagcga tggagtcgtg gattgctaat ccacaattac
     4981 tcaaggctga tgccgatgcc gattattacg aggtcattga gatcgacatg aacgagatca
     5041 aagagcccat cttggcttgc cccaatgatc ctgacgatgt gaagttcttg tcggaagtac
     5101 agggtgacaa gattgatgag gtgttcattg gttcgtgcat gaccaatatt ggtcacttcc
     5161 gtgctgctgg caagatccta gaaggtaaga ccgatatccc aac
//
LOCUS       DDPQ01000114            3182 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_23473,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000114 DDPQ01000000
VERSION     DDPQ01000114.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_23473"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1165..1221
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          851 a          735 c          691 g          848 t
ORIGIN      
        1 agtattgctt catcgacatc atgtgtgatc atgaatacgg tctgtttggt agatgagcaa
       61 attttcagta attcatcctg aatcacccca cgggtcagtg catctaaagc gccaaagggc
      121 tcatcgagta aaagcatttt gggttcaatg gcaaatgctc tagcaatacc gacccgctgt
      181 ttcataccac cggagagttc tgccggcctt ttatgtttgg cggctgttaa tcccactaac
      241 tctaaatatt tctcagaatg agcttctatc cttgctttgg gccaatccgc aaatttagat
      301 ttgaccgcaa agttaatgtt ttgcaatacg gtcatccatg gcatcaaggc atgcccctga
      361 aagacaaccc cacgttctaa gcttggacct ttaatttctc gaccgagcat gtatgcatag
      421 cctgatgttg cttcttctaa tccggccaag acatttaaga tagttgtttt gccgcaaccc
      481 gagtgaccaa tgatacaaac aaactctccc tgggtaatat caaagttaac attctcaaaa
      541 acaggtgcag cgcctggcac ataggtttta gacagacctt ctacttttaa aaattcgtga
      601 ttaatctgca taggtcacca tcttttgtag tttggcaaag aacatatcaa gcaacatacc
      661 cacaaagcca atcactacga tcgcaaaaat aacgctagag attgctaagt tattccactc
      721 attccagaga aagtaaccaa tcccagtccc gcctaccaac atctctgcag ccacaatcac
      781 aagccatgca atgcccattg agatgcgcat tcctgtcaga atcgttggag cagcagcggg
      841 aagaatgacc ttgaatgctt tacgaagagg gctgacctct agagtctttg cgacgtttaa
      901 caattccttg cgaacgctcg caactccaaa ggcagtattg atcaacatcg gccaaattga
      961 gcagatgaaa atgacaaaga cccctgatat tgaagagtcc ttaatcgtat agagagcaat
     1021 gggcatccat gctagcggtg aaataggctt aaggatctgg ataaagggat taaaggctgc
     1081 tgagagtagg ggcgacatcc caattaagaa cccaactggt agcgccacca gaagtgcaag
     1141 caagaaccct aaactaactc gtgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nccgttgagg ttgtggtcgt tgcctcggct tttgagcccc
     1261 cctttggcat agttgctaga tgccagaccg ataacatcat taaaaaaatg atgaccgaaa
     1321 ggagggcgcc cttcacctta atggacactt tatccatgat tcattaaccc ttggatatcg
     1381 caaaggactt gctataggcc gcagcagttc caggatcaaa ctccttaccc atgatcttga
     1441 actttggatt accagtactt gcttttgctt gagcagtaat cggcgcattg gtctcttgca
     1501 tgtatttctt ggcatcagtc aatagaaaga ctttctcaga gatatctttg taattgacat
     1561 cgcctttgac atagccccag cgctgcatct gggtcaacat ccaggttgcc attgagtacc
     1621 acgggatagg attgaagtcg atacgatctg gtacgttttg aacattgccc aaaccatccg
     1681 caaacttacc agtgagaacc tgcatcacaa cggtttcagg ctgattgaga tagttttgtg
     1741 gggaaattac cttagcaatc agtggacgat tctcaggatt gcgcgccatc gttgatgcat
     1801 taatgactgc gcgatagagt gctgcaaagg tattggggtt cttcttaata aattcttcgg
     1861 aggtaccaaa cgcacagcag gggtgtccgt cccaaatttc tttagtcaaa atatgaataa
     1921 atccaacttc atcaaaaact gctctttgat taaaaggatc aggccctaag aatccatcaa
     1981 tattgccagc acgcaagttg gcgaccatct ctggtggggg cgttactcga atctggatat
     2041 ccttatctgg atctagtcct gactgagcaa cgtaatagcg caataagaag ttatgcatgg
     2101 agtactcaaa aggaactgca aacttcatcc ctttccacat cttggggtca cgtttatcct
     2161 tgtgcttatt cgctaaggta atagcttgcc catttgtatt ctggatcgtt gctacccgca
     2221 tttgtcttgg gtcggagcct aggcccatcg acattgaaat tggcatgggt gataaaaagt
     2281 gcgatgcgtc atgctctctg ttgagcatct tgtcacgtat taaggcccaa ccagcagtct
     2341 tgtttagggt gacatttaaa ccttgttttt tataaaaacc caaggggtca gccatgatta
     2401 gtggggtagc acaggtaatt gcaatgaagc caatgctgag atcttttttc tcaagcggcg
     2461 ccttctcttg cgccatcgct tgtaaggagc caacaggtaa gaagcctgag agggcgctca
     2521 tcgcaccacc agttcctaca gcttttaaga atgcacggcg acgggcttct tgcggaaaga
     2581 gtgcttttac taggctagct tcaatgaagt tagcactatc ttcctcaaca tcagctttac
     2641 gtaactgagc agcgtggtgc tctgactcag aggcgtgtag gccacaacta caacggctac
     2701 taaataaagg acgatcagga tcaaacggct tatgaatact cattgcaggc tccttgaaaa
     2761 gttggttact gactctcatg caaaaaacat accaacccat cgtattgggc actgcaaggg
     2821 cttattgagt attagcgctt atttcttgtg cgcgtgtaga ctagattcac gctattggtg
     2881 cagtttttgc accagactag ctagatggac tgcgtttgcc ttagattggc aatctcactg
     2941 tcctgatagc ccaactcttt taggatggca accgtgtgct cacccacgct gggtatctta
     3001 tccatgcggt attgataggt gttattaagc ccgggtggaa gcaaggcagg tatttccccc
     3061 ttcggggagt ccaccattac ccaacgttgc cgggctttga gttgctcatg atcccaaaac
     3121 tgttgcatat cattcaaaga ggcattcgca atctgtgcct catcaagctt ggcaataact
     3181 tg
//
LOCUS       DDPQ01000115            4935 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_24271,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000115 DDPQ01000000
VERSION     DDPQ01000115.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_24271"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2040..2142
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1279 a         1149 c         1173 g         1231 t
ORIGIN      
        1 tgccaaaaaa gcagctaaga agcctgccgc taaaaaagct gctaagaaaa aagtagctaa
       61 aaagcgtcct gctgctaaga agaaaaaagc tgccaagaaa aagcctgcta aaaaagcagc
      121 taagaaaagg cctgctgcca aaaagaaagg ggcttctgta aaaaagcccg cggctaagcc
      181 cgccgttgct gaatcagcgc acactgcgtt gaacccggct gcggcttggc cttttcctac
      241 aggcagccgt ccctaagcga aagctgaacg gacagttgag ctagtactga tggggtcctt
      301 cgggacccca tttttatggc ttagaaagaa tagccaaagg cgtctttaaa acgtgtcatg
      361 atgtcggagc gggggggttg atgattttgg ggcccttgat gagcgatttt gattgaaccc
      421 attaggctgg caagtcttcc cgtggtctcc caatctaatt tattttctag accgtagagt
      481 agtcccccgc ggaatgcgtc accgcaaccg gttggatcaa tcactttttc cggtctgacc
      541 gcgggtatgg caatactttt gccatcggca tagatttgtg ccccttgcgc ccctttggtc
      601 acaatcagcg cttttacttc cttagcaatt tgctctaaag ataggccagt gcgctgacaa
      661 agcatttcac cttcatagtc attcacggca acgtaactgg caagctttac aagttcgaga
      721 agctcttgac cattaaacat tggtaaacct tggcctggat caaacataaa gggtacgcct
      781 gcctctacaa attggtgaca atgctcccac ataccaagcc gtccatcagg cgctacgata
      841 ccgaagtgtg gactcgcgga accaagagta ctaaggcagt ctgtaacgcg attgaggtga
      901 gagtgattca tggcgcctgg gtgaaaggca gtaatttggt tattggccat atcggttgtg
      961 atcatggctt gtgcagtgaa ggcctcatcc agtttcttga catgggatcg gttcattcca
     1021 agatcatcaa ggcgctgaag gtaggggtcg gcatctgccc caaccgttgc cattaccaaa
     1081 ggctcacccc ctagtagttt gaggttgtag ccaatgttcc ctgcgcaacc accaaattct
     1141 cggcgcatgc taggtaccaa aaaggctaca ttcagaatat gaatctgatc gggcaagatc
     1201 tgatcttgaa aacgaccttc aaaggtcatg atggtgtcgt aggcaataga gccacaaatg
     1261 atgctagcca taaatacttt ctgtcatgga ttggtgggag attgagtggg atatgctaca
     1321 cgcaggcgat agcctgcggc attcgaggga acttgaaggg ggattgatag gttaaatacc
     1381 tcccccgctg atgcgccggt caataaaaaa tcaggatgtg tgctttgcca ggtcttcgga
     1441 agccattctt cgggagaaag aagtatgcgg gcaatagttt tctcatccgc atcggtcaaa
     1501 atgagttcga gtccaggaat ggcgatgggg agagctaagc ggttttgtaa ctcgatttgt
     1561 aacatcgatt gcgcggctgg caagccagga tcacggttgt ttatttctgc aatgcctaga
     1621 ttggccgata taaagcgcca tgctttgaaa tcagcaatag ccttacgatc acatggaatc
     1681 cattcacata aaaagttatc gatcgtttga acggctctga aagcagataa cccgattgag
     1741 ttaggactgc catccatgct tggggcaagc gcatgaagca taggcattcg ccagaacgct
     1801 gtagcaaatg gataagcgat caaaagtact gctagtccag ccaataattg ttttttgata
     1861 gaaagaggct tttttatgag gcccgatttt gtaacgatat gcttcaatcc tgagccagac
     1921 aatttttttg gttcgtcatg aatcgatcgt ggggagctaa acgccatatt ttgagcttta
     1981 gggctcaatt taccgattag gcatacccaa ccctcatcga taccccagac agagagttgn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcactac atcaaatact
     2161 tgattgccaa cggcattctc gatatcgtcc ggcaattgaa attgaatccg cgtttggttt
     2221 tgttgagcat ttgttctggc tgattcaatc gcctgtggat caatgtctgt accgaatact
     2281 tccccaaaac caagtttgta tgctgcgatc gctaggattc cggagccaca accatagtct
     2341 aagaggctct gactgtttag tgcagattct tggctacatt gctccagcca ctctagacag
     2401 agtcgggtgg tgggatggct accagttcca aatgcgaggc caggatcaac tgctaaacaa
     2461 atggcatccg gatcgctagg tgtttgatgc caggacggaa caatccaaat tcgcttaccc
     2521 acgtggatag gctcaaattg actttgggtt aaacgaaccc aatcctgatc tggaacgatg
     2581 acttcttgag gcgggtttac tgaaaaccca gcttccttga gttcggcaac taaatcatta
     2641 aggggtagat ccagatcttt actaaagaga gctttgacac tcgaaagatc ccatgcttgt
     2701 acctctggtg aaaggccggg ttctccgtag aggggatttt catcatatcc gccggcagct
     2761 gcatcttcca ccgaaactga aagagcgcca atttcgatta gggcatcacc taagggctca
     2821 gcaatttcag cacctaccgt aaattggagt tcgcgatacg gcatctaaat tagagaatca
     2881 aaacaagtaa ttatttcttg ccgcgagtag ctgactgctc ctcaagacgt tgctcaagat
     2941 aatgaatgct cgtgccgcct tccacaaatt ttgagtcgag catcagttcc cgatgtagcg
     3001 gtacattcgt ctgaatacca tcaatcacca tctctgagag tgcaatacgc attctacgaa
     3061 tagcttgttc tcgagtcgcc ccataggcaa tcaatttccc aatcatcgaa tcgtagttag
     3121 gtggcaccat gtagccgctg taggcatgag aatcaactcg gattccaggg ccgcctggca
     3181 tgtgccagga ctgaatccgc cctgggcttg gcgtgaactt gaaggggtct tcagcattta
     3241 aacgacattc gatagcatgc cctctaaaga taacatcgcg ttggcgaaaa ctaagttttt
     3301 gcccagccgc aatcttaatt tgctcctgca caatatcaat acccgtaatc atttcagtaa
     3361 ctggatgctc aacctgaacg cgcgtgttca tctcaataaa aaagaactcg ccgttttcat
     3421 ataagaactc aaacgtaccg gcaccccgat aggcaatttt tttacaagcg tcagcacagc
     3481 gctcaccaat cttagcaatt agttttcgat caatacctgg tgctggagcc tcttcgataa
     3541 ccttttggtg gcgacgttgc atggagcaat cacgctcacc aagccaaagg gcattgccgt
     3601 gtgtatcagc taacacttgg atctcgacgt ggcgtggctt ttccaagaac ttttccatat
     3661 aaacctctgg attaccaaag gctcgtcccg cctcttcgcg ggtcatattt acggcactta
     3721 aaagagctgc ctcggtatga actacgcgca tcccacgacc accaccacca ccggctgctt
     3781 taatgatgac ggggtagcca acgcgtttag cagtcgcaat aatttctttg gggttatcgg
     3841 tgggtaatgc ccctgccgaa ccgggcacac agggaacccc ggccttgatc atggcattct
     3901 tagcagaaac cttatcgccc atcagacgaa tggattcggg gcgaggaccg ataaatgcaa
     3961 aacccgattt ttcaacacgc tctgcaaagt ctgcgttctc tgatagaaag ccatagcctg
     4021 gatgaattgc ttcggcatca gttacctctg cagcagaaat gatggccggc atattaaggt
     4081 agctctgacc tgagggggct ggcccgatgc aaaccgcctc atcggcaagc tttacatatt
     4141 tagcctcttt atcggcagtg gagtaaacca ccacggtctt gataccaagc tcacgacagg
     4201 cgcgctggat gcgtagggca atttcgccgc gattagcgat gaggattttg tcaaacatga
     4261 tttgggcttt ggtgtggatt aggcgatgat aaaaagaggt tgatcgaatt caacaccttg
     4321 accgttttca catagaactt gtttaacgat gcccgctttc tcagattcaa tttcattaag
     4381 gagcttcatt gcttcaatga tgcacaaggt ttgcccaacc gttacggtgt cgcctacatt
     4441 gacaaaagca ggtgcctcgg ggtttggtga gcgatagaag gttccaacca tcggcgattt
     4501 ttgggtaaag ccttctgtcg ccacgctggg tgcagtagca acagcgatag gggcggggga
     4561 ggagggttct agcggtatcg gtgccgccat gacaacggtg ctggggctag agccagcata
     4621 agtacttgca gttgggccgc cccgtgtatt cacaatacga actttatcct caccctcgct
     4681 aacttctagc tctgaaatcc ctgaatctga gactagatcg attaatgtct ttagttttct
     4741 taagtccatg tcaaacctcg tattgaagga tgtttatgtg gaagatagtt tgtgttgaat
     4801 cgtattgagg gcaagttcat atccatgagc ccctaagccg caaataacac ccatggctaa
     4861 atcggaaaag taggaatgct tgcggaactc ttcgcgctga tggatattgg ataaatgaac
     4921 ctcaataaaa ggaat
//
LOCUS       DDPQ01000116            4819 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_33104,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000116 DDPQ01000000
VERSION     DDPQ01000116.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_33104"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    129..146
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1730..1767
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4578..4601
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1263 a         1092 c         1080 g         1304 t
ORIGIN      
        1 ttcatggcca taccaagata gatgggcttg ccgcggattt ggcttatgac cgtattggga
       61 tcattaaagt cgagcttaaa gaggcaaata atcctggaaa gagtagcttc gtcaacccgc
      121 tctttgttnn nnnnnnnnnn nnnnnntaga gggagttcaa tatctcggtt gcagaggcat
      181 ccaagccaac atgccaaacc cacttgggat cattaatctc ttttagagga gtaatcgtag
      241 tcgctatccc caagaaatga ttaatccatt tctccaaaat ttttgctaat gcaggcaatg
      301 tcgcttgatc aaaattaagc tggattgcaa aatcatgtgc ttgatcccta cccaaatagg
      361 atttggcatt gtcttcgtgg aggacatcta gatctaccgt acgcatggcg atggactgac
      421 tctttagaag gtcaataata ttgccgaact cactcgcctg agcattacgc tcaatggtct
      481 catggtcagc tgccatcaca atgccatcct ctaagatggt gatcttttgt ggccgaaaga
      541 agagttctgc gattcgggca tcaaaagggt ttacttcggt accaaggata tgtctaagaa
      601 agatttgggt gagttgcgca acgaataaag gtggcacatc aacaccctca ccttcaaaca
      661 aactcatgta gaagttctca agcgagcttg cggctaatag tttggtgcgg tagcgaaacc
      721 aaatcgcata attagcctga atatctgcat cagccatctg ctcaatctct gcttgtgcca
      781 cttcttcacg gggattgttc aagagacgct gatgaattgc tctttctttg ggacaagatt
      841 ctggaaccaa ggccaactca ggtcttgata aataggttct taagaaatca tcggtcacca
      901 atagttggtt atcgggacta acttgcaggg tatcaaagcc acaatgggcc cagtaatttg
      961 gcatagggtc agtaattaaa aataatcagt gctattgtcc actagatcaa tgccgcatga
     1021 ttaacaaggt catccccgca ttcccctctt tcaattgctt gaggtgctgc tttagcgtgg
     1081 ggcgacgttt tatgagggtc tgataaagct cactacgatg cactaaatct tctccatgat
     1141 agtactcatc aactcggtct gagaaaaagt tgttctcaag agcttctcga acagtgcgtc
     1201 gtatgcctcc tccctgtccg gatgagccat agccgtggat gacgatcaat gcacgtattt
     1261 gcacatcagc agcagctcgt atatggtgag taagggtatc aagcgcctct tcacgcgtcg
     1321 gtccaccgat ctccagattt acgattgcag tatccgtatt tgcacttggt acctcttggc
     1381 tttcgcagaa ggggcaatcg cccagcatcg ctcgggggtt accgcaggtg gggcactcaa
     1441 atgaaggctt cacgtttact tcaccgcttt ataaacgccc atctcctcta gtaactcatt
     1501 gaccagatca agtctgagtc gggggttctc ttgttcgagg aaaaactgtt tttgcccagg
     1561 gctaagttgc aggagttcgc accagcgatt ggcaacccaa ccgcattcat taaagcgata
     1621 gggtttatga aagggtaatt ctttaccgac tgcagcaccc tgctccttga atgactggat
     1681 tactttaccc aaaacctcag atgcgggttt gagctcgtca ggaacttcgn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnncta ggttgtactg gatcaaattc tttgatgcga
     1801 gcaagagtac cgacgtcatt aaaactaacc tgctccccgg gtattcgtac ttcagttcca
     1861 ccatttaggg taaccactcc aaatccagag ccctcacgaa cacattgctt gaccatatct
     1921 aagtaacgca cctcaaagat ctttaataaa agaataccgt ctgggtaaag agcggttcct
     1981 aatggaaaga tgggaatagt ttggacgtgg gtataaacgg acataaacga tttactctat
     2041 ttaacgaaca cattgataaa tatagatcat tggaaactga tcattgtggg tggtcttggc
     2101 ctcagaaatc gctgcagacg atctgcccat ctttttgcac tctttgactg ccagttcatg
     2161 ggcctccagt tctttggcgc ccttaattgc ttgcacacca atcatgccat cggaccgatt
     2221 cacaacccga aatccacttt ggggagtgcc acaggccgtc agcaggaaaa tcaaggctaa
     2281 agaaacgagg cttggggtgc tgggcttatg catgattatt actatactgc tatttgttaa
     2341 acacactggg agacgaccat gaatcgcgat gcatttgaga agggtttaaa gacacgccgt
     2401 gaggttttgg gtgctgagta tgtggatcaa tccatccaga atgcggatga gttcaaccga
     2461 ccaatgcaag agttggttac tgaatattgc tggaacgaga tttggaatcg tcctgggctt
     2521 gatcgtaaga cccgcagtat tgtgaacctg gcaatgatta ctgcactcaa tcgaccccat
     2581 gaactgaaat tacacatcaa gggcgctatc aacaacggtc ttaccaaaga tgagatccgt
     2641 gagatctttt tacaaaccgc catctactgt ggtgttcccg cagcaattga tagcttccga
     2701 aatgccaaag aggtatttaa agagatgggc atctaattgg catcagccca ttctgaacca
     2761 atcacccaat cttgacgatg cgagttgagt ttgctttaat ggatcaaaag gagacctcta
     2821 tgacctggca gcgctctcgc cttaccatct ctctaatagc tagcgccgta cttcttactg
     2881 cgtgcgccgg tgccgaggtg cgcccaattg tggatatgaa tggcgtaaat gaggcgcgct
     2941 atgagaaaga tttagccgag tgccaagatt atgcaaagca ggctactggg atgggcggca
     3001 ccgccgcaaa gggtgcagga gccggggcag ttgttggcgg acttctaggc ttagtaaccg
     3061 gaagtaatac aacagggatc gttcaggcag ctggtgcagg tgcggtcatt ggtggtgctg
     3121 gtggtgctta taaagggaat gactcgcaag aagcggttgt taaaaagtgt ctcgttggtc
     3181 gcggatataa ggtccttaat tgatgtaagt ctgatgtgtt tagcttatgc gtctagttat
     3241 tgtatttact gcccttctta cctgtctgct aacaagccct gcgcacgctg gtttatttga
     3301 tcgcccaaag gatccagccg ccatgatgct taatcagaaa gactgcacct atatgaaaac
     3361 ctaccggggt aagacggtca atggagagtt tattcgtcct gaccgttggg tcggatcaaa
     3421 atttagcatt ggtgagccca caatcattgc aaatttcaag cgtttagatg agaagactgc
     3481 ccactttacg aatgtgaata atattgggga tggccaagga gtgagggtgt atgtgggcaa
     3541 aacgaccgat gctttagagg ctcgactatc cgatggctca ctgattctgg tgtgtcacct
     3601 gaacaagtag ttatattttt atctgtggat cggccgccat aatctcttct cggccacaca
     3661 agcgcatcga ccaattagca aagcgccgag tattaatttt cttcatacct aaagaaacta
     3721 cattggtatg acggggatct ttcagaatta gctcccagat tgcctcaaca tccttgggct
     3781 tcccttccaa gatttgtcca aagcgtccat gatcaaagga caggacaccc gttacattta
     3841 gggccttatt acgacgtgag gccttatcca ccatttgcat caaacttaag atgccgaatt
     3901 cttccgctgc atcactgaca taggacaagg tcactaaatc aagttcggtg taatttcctt
     3961 ccggaaagta tttggtggct ggttttggca caatgacttg atattagctg aatccttaca
     4021 atacgcaatc accttaggaa aaattaattt ttatttcctt ttaatgcggt ctcaagctca
     4081 ttaatcttat tttgcatcgc agcaatccta gcgttgagca tcccaacgtt cagataggtc
     4141 tcatcagcaa cgggggaggc taaatgactg gcccacccta accatgacga gtcatatatc
     4201 ttgacgttct tgtatccaag attttgtaga acggttgcgg tctcagcggc acgtacccca
     4261 ctttggcaat aaaccactgt ctctttattg gggtctaatt ttgcatacag cgcattgagc
     4321 tcgtcttttg tttttaaaga catcccggca tttgttttga cttctttctt agcaagcttt
     4381 gatccggtgg caggatcttt ccagttttgc tcatacggaa tactaatcgc gccgggtata
     4441 tgccctcccc gtatcgcacg aatatcatta cccttaaact cattaaaggt tctcgcatcc
     4501 accacttgaa ccgattgatt gttatagagc ttacgcattt gctcattgct aaccactaag
     4561 ttttcctgag gggctaannn nnnnnnnnnn nnnnnnnnnn nccatcaata ccatcatgaa
     4621 aaactttggc ctgcttaccc ccgaaataat ggatcgtgaa taggccaaaa taggagtaag
     4681 gattacctct cgagccatac accacgatct ctttataaat atcaagccca gccttattaa
     4741 agattgcatt gatctccgct aaatcaataa aatcctcgcg attggggtct cgtaaaacag
     4801 ttccgatatc gccgaaatt
//
LOCUS       DDPQ01000117            9859 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_8128,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000117 DDPQ01000000
VERSION     DDPQ01000117.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_8128"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    4136..4191
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2476 a         2160 c         2531 g         2636 t
ORIGIN      
        1 caagctggtc tgggggttgg tcgtgtcgtt cggagcaaac cagtcttcca gcaaaattgc
       61 ttcctgcaga atgcagggct ggtaaataag gtagcgatgg cgagctttta attaactcgc
      121 cttccactct ccagatttac caccactctt ttctagtaat ttgatatcac tcatgaccat
      181 gccacggtcg actgctttgc acatatcgta gattgtgagg agagcaactt ggactgcggt
      241 cagggcttcc atttctacac cggtctgccc agttgtttct gccctcactg tgcatcgaat
      301 ggtgttggtt gtaggcatgg actcaaattc cacgctgaca tgcgttaggg caagagggtg
      361 gcacaggggg atcaaatcgg aggtcttctt ggctgcttgg atgcctgcga tgcgagcaat
      421 tccaaggaca tccccctttt tatgacctcc cgcctgaatc aattcatagg tggtagcatg
      481 catttgaata caaccactag caatggctat tcggtgggtg gaggccttat cgccaacatc
      541 gaccatatgg gcttggccac tggaatcaaa gtgtgtcagt ttgttcatag gataggtttt
      601 accatatagg gatgggtatg caattgagac gtattttggc actttactta agtttgatct
      661 tgagtgcccc ctcttttgcc caacagagcg gagtttccgt tcaacaagac tcagccgctc
      721 agcaaaactt ggggcgcgtc gtccagtcac ctgaggcccg ctcccccaat ttaccaaccc
      781 gtaataccct gcaatctcaa cccacactta tattgcctga tatgggtgat ccgggtggcg
      841 atgcgctcag ccctttggat gagcgtaaga tcggcgagca aatcatgcgc gagattcggc
      901 gtgatcggga tttctcaaat gattggccaa tctacgacta ccttaatcaa atggagcgcc
      961 gcttaatgca agctgccaaa cgattgcaat tgggcggcgc caacgctcag gggagtgctg
     1021 cgtatgacta tgaggttttt gcaatcaaag atccatcgat taatgccttt gcattacctg
     1081 gtgggtttat tggctttcat acgggtttgt taattactgc agagaccgac tctgaggtag
     1141 cttctgtgat ggggcatgag atcggacacg ttttacaacg ccacatcgct cggtctctag
     1201 agcgtcaggg cacaaattca attattgcct tggcaggtat tgttcttggt gcattggctg
     1261 ccgcaagtaa tccaagcgct gctgctggat taattactgg gggtcaagcc ttggcgattc
     1321 aaaatcaact cgcatactca cgcgatgcag aacgcgaatc cgatcgcatt ggttttcaga
     1381 tacttcaggc cagtggttat gatgtaaatg gtgccccagc attttttcag cgcctgcaaa
     1441 aattctacgg catcatggat agcggcgtgc ctgcatatgt acgtacacat ccacttacga
     1501 tcgaccgaat tgcagatatg caagatcgtg ctcgcacgat tcagcaaagt cgggttccca
     1561 gtaccttgga gttttactta gttaaggcgc gggcgcgggt tgagcaaagc ggtagctcaa
     1621 gtggtttatt tgatctacgc aatgtgtttg agagttattc caagcaagct gctcctgaga
     1681 agcaaatgga gggcttttat ggcttagcat tggttgccca gaaacagggc cgtcttgaac
     1741 aggcggaggg ttttttacaa aaggcgcgag ctgtagcgca gaatgtactc gcaccaggat
     1801 caccagtgca gcgtcaaagc ctatcctttg attacactgc ctcagaattg gccttggcga
     1861 gaggaaaggc agaagatgct ttgcagtttg cacaaaatgc tgctaaagcc gacccatatt
     1921 ctttttctgc ggttgtagct atggttaagg cagagctcaa attaggtcga acgaatgatg
     1981 cgatcacact tcttcgtgct aaaacaaaag ctcaacccaa tgaaacaatt tggtgggagt
     2041 tattagcaag tgcttatgac caagataaaa agattggcct acggcattac gctcttgctg
     2101 aaaagtttgc taccgagggt gcctggccat ctgctattga gcagttaaaa attgcacgtg
     2161 ccgctgctgg aactgacttt tacttaggct cagtgattga tgcccgctta cgagcgtttc
     2221 agaatcaata ccgcgaagaa caaaacgaga aaagaaagag ctagtgcagt tcgtgttgat
     2281 cgttcggaaa ctcaaaatca tcatcgtgtg agcggctggc atgtaaatgt gcaattcgcc
     2341 agcccttacc gtcttgcatt agaaccaaag taatattgat caagaaatct gcttcaattt
     2401 gattgttctc aaaatagact gcttcagtgg aatcaaagat agccgcaccc atagtgatat
     2461 gttcaataca ggcaatgggt tctagataga gtggtttact caacatccgt tctatacccg
     2521 agcggatttc agcatgtcca ctgagtcgat tgccttcagg tagcacacag gtaatcgaat
     2581 catcatccag ccagagcgct aaggccccct ccacatcggc ttggcgcaag gtctcgcgcc
     2641 acgcatcaac aacgtcgtct gggttctgaa aaagtctggc aaaacgtggc ataaggatct
     2701 ctggctggtt aagctatagc atagcgtaga cctgcttagc ctcaatttta gttaaacaat
     2761 caagatgccc taaggggcag tttcgttggt agcaaggact gcaggctaag ccaagccaaa
     2821 caaccttcgc ttgcttggat aggggtggcg tatgcttagg attactagac ccaaagattg
     2881 caatttgcgg aatcgctaga gctgcagcaa tgtgcataag ccccgagtcg ttgctaatga
     2941 tttgtttgca ttgtgcaatc actgccattg cttcttcaag actaatcact ccacaccaat
     3001 tatggatccg ctctgtaggc ttggtatggg cttggatttc ttgggcgagc gagtggtctg
     3061 ctttactccc caaaataacg atctgggcat ctgtatcctt acctaatatt tttgtcgcca
     3121 gctcggcaaa gtgtgagctt ggccagcgct ttgcaggacc atattccgct ccaggtgcaa
     3181 acacatatag agttctagta cccaatgcat cgatacgcgc tttcacttcc tgaacacctt
     3241 catcgctaat agaaagtttg ggttgtgatg tatggtcaaa ggatggagat gagttttctg
     3301 aaatcttcag taattggccg taatgctcca ccatcggtgt gcgtgcctga cgtgatgggt
     3361 tagcgagaac atgattaata aggccgaagc gaagttcgcc ccgatagcca atacgaattg
     3421 ggataccagc gagccaagga attaaggcag acttaaaact attcggtagg acaaacgctc
     3481 tgttataggc gttcgcttta atttgcttag caatggagcg acgtagatcc cattgcaaaa
     3541 tgccatgctg aaaatcaacc tcgatgacct tcgtaatctc cttgcaagct cgatagatag
     3601 gagcaaccca aggcgtgcaa agaacatcaa tcgtttgtga agcatttgtt tgtttgagac
     3661 cagcgagtaa aggctgcgac ataaccgcat caccaatcca ttgaggggca atgatgaggg
     3721 tacgttgcat agaaaaagcc ctccatggag ggctgttatt aatggccgtg aggctcggta
     3781 ccagggatga gtcggtatat cgtgccgcaa tatgggcagc gtacctcgcc agtttctaaa
     3841 acatctagaa agacgcgtgg gtgatagttc cacgaaggag tatctttggt agggcaatgg
     3901 agtggcaaat cattgccatg aatcatcacc gcatttagtt ccttgagttc gctcatctta
     3961 actagacagg acttaaccac tcacggtagc gatcatttct gccatatacc gtgtcgaaga
     4021 atacatcttg gatttgtttg gtgatcggac cgcgcgtacc gtcgccaatg gtgcgatcat
     4081 caagctcacg aattggggta acctctgcag ccgttccggt gaagaaagcc tcatcnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtaatgccg
     4201 tcaagacagg atgcgagatc tggagtaaag acaatgccat tacgaaccat gaacatattt
     4261 tcaccagaac cctcagacac atagccttcg gtgtctaaga gcaatgcttc atcgtaaccg
     4321 ttcgcagcga cttcttgatg agcaagaatg gagttgatgt aatagcctga ggcttttgct
     4381 ctcaccaagg aggagttaac aaaatgacgg gtgaaggaag aggtcttgac ccgaatccct
     4441 ttattgagac cgtcttcacc caaataagca ccccattgcc aagcggcgat ggctgtatga
     4501 gttgagttcc ccgtcgggga gatgccaagc ttttgtgagc cgataaaaat aatcggacga
     4561 atatagcaag atgctaattt gttatcacgc acgacctgag taattgcttg ggcaatctgt
     4621 tcttcggtgt aaggaatttt catctgaaaa attttcagac cattaaaaag acgcttgacg
     4681 tgttctgtga ggcgaaagat ggcggtacca tcttgcgttt tgtatgctcg gattccttca
     4741 aacacgccca tgccgtagtg caggctatgg gttagtacgt gaacattggc atctctccaa
     4801 gggacgagct tgccatcaaa ccaaataact ccatcacggt cagacatcga catctttagc
     4861 accttttatg ttgtgggcgg gctggctagc gctagaaaac tggcataagc cattaaaagt
     4921 cttattgtag agcagagaaa tggcggggga cggctttgaa acaggcttgc tgaaccgggg
     4981 ggtttctata atgggggcct cctatgcgtt ttaatccatt cgtttccaag ttaccctact
     5041 ggggctggac ccctttgcaa caaggggctt ggcgagacgg actaaaaacc atgtcgacct
     5101 cagcagtgac catttttagt tgggccttag ttactgggct ggcaatgggt aaatccccat
     5161 tgagcacaga gcaggcccta gcaatgagcc tctttgtgtt cgcaggaact gcccaattgg
     5221 cagccttgcc cctaattgct ggcggattct ccatcggtac gattttgatt actgccttcg
     5281 tcgttaatct gcgcttcatc atttttagtg ttggcgttca ggctcatttt tctcatttgc
     5341 cttactggag aagggtgatc ttggggtatt tcacggcaga cttcggctat ctcatgttca
     5401 caaaccgctt tactgaagtt caaaacgctc aggagagaaa ggctgacggc tattaccgaa
     5461 gccactttat gtatggcttg gcttgtggta actggaccat ttggcaaaca ggctcaatta
     5521 tcggtatttt gtttgccagc cagattccgg acagttgggg attggagttt gccggtactc
     5581 tagctttaat tgcagtgatc gtaccgatgc tagatcatcg ctcggctcgc tgggcggcag
     5641 gtgctgcagc ggtcgtcgct gtattggcgt attccatgcc atttaagcta aaccttactt
     5701 tggccattat tgcggcaatt atggtgggga ttcttgcaga tcgatctccc aagggggcgc
     5761 aatgaggcca gagcctttaa gttctttaga aatcaccttc ctaattctag ggatggccat
     5821 cattactgga ttaacccgct cgttcttcgt atttttaggc gatcgggtca aggttccaga
     5881 gctcgtgcta cggtccatac gctacgcacc gctggcagcg attgtgggca ttttggctcc
     5941 ggagcttgta ttaccccctg gagcccttga gattagccag ttggactgga aattacccaa
     6001 tatttggggc ggtattgctg cttttggggt gtatttttgg acccgccaga tgctaccgac
     6061 cttattggtc ggaatggcag tatttagcct ggtacgccac tggatctaaa atggaggact
     6121 ccaaccttcc tagatgaata gtctgtttta tgcttactcc gcttctcaaa gttaaacgct
     6181 taaatgaact tgctgctact ggccagttaa aagggaaacg tgtttttatt cgtgctgatt
     6241 taaatgttcc gcaagatgag gcaggcaata ttactgaaga tacccgtatc cgcgcatcaa
     6301 ttcctgcaat tcagatgtgt ttggatagtg gcgccgcggt gatggtgaca tctcatttag
     6361 gaagacctag tgagggggct tttaaacccg aggacagttt ggcgccgatc gctcatcgca
     6421 ttgctgagtt actcaatcgc aaagtgccac taatcagtaa ttgggtggat ggtggctttg
     6481 atcttgccgt tggcgatgtt gttattttag aaaattgtcg tgtcaatgta ggtgaaaaaa
     6541 agaataccga tgaactagcc aagaaaatgg ccgcactctg tgatgtgtat gttaatgatg
     6601 catttggaac agcacatcgt gccgaggcaa cgacctatgg catagcgcgt tatgccaaag
     6661 tagcttgtgc aggcccgttg atggctgctg agcttgacgc tctaagtcgc gctctggcag
     6721 aaccaaagcg tcccttggtt gctattgtgg cgggttccaa ggtctctacc aagttaacga
     6781 ttttgaagtc attggccgag aaggttgatg agttgattgt gggcggtgga attgcaaata
     6841 cttttttatt agctaagggc ctatcgattg gcaaatcctt ggcagagcct gatttagtcg
     6901 atgaagcaaa agaaattatt gccttgatgg aaaagcgggg cgcgcgcgtc cccattcctg
     6961 aagatgtagt agttgctaat gagctttcgc ctttagcgcg tgccaatcga gtgccggttg
     7021 atgaagtggc ccaagacgac atgattttgg atgtaggtcc caaaacagca gcaaagttgt
     7081 cgatgatgtt ggcccatgcc ggaactattg tatggaatgg cccattagga gtttttgaga
     7141 ttgatcagtt tggcggtggt accaaaatga ttgctgctgc aattgcccat tcaccagcct
     7201 tttctattgc gggtgggggt gatactttgg cagcgatcgc gaagtatgga attgagaatc
     7261 aagtggatta catttcaact ggtgggggcg ctttcctcga attcctcgag ggtaagacct
     7321 tgcctgcatt tgccgtgctt gctgagcgcg ctaaggacta atcatgcaac gagcaacaaa
     7381 aattattgct actctgggac ccgcttctga gaaacccgag gtcttgcggg acatgatccg
     7441 cgcggggctc aatgtggtgc gcctgaattt ctcgcatggt acggttgctg accaccgcgc
     7501 gcgttacgat ctggtgcgac aaatttcaaa agaagtggat aaagaggtcg gcatcatggc
     7561 tgacttgcaa ggcccaaaga tccgggtggg taagttcgcc gatggaaagg ttgttcttaa
     7621 agaaggcgct caatttattt tagatatcgc ctgcgaactt ggtaatcaag accgtgttgg
     7681 tttggactat aaagatttac ctaaagatgt aaagtcagga gatcgtttac ttctgaatga
     7741 cgggctgatt gttttgatag tcgataaggt tgtgggtggc gagatttata ccaaggtgga
     7801 gaccggtggc cccttatcga ataacaaagg aattaatcga gccggtggtg gtttaacggc
     7861 cccagcctta acagaaaaag atattcagga tatggacgcc gctatctcaa tgggcgttga
     7921 tttcttagca attagctttc ctaaagatgg cgctgacatg gcctatgccc gcaagcttgc
     7981 cgatgctgcc agtgtcaagc atggtaaggg caaggcacgt ttaatcgcca aggttgagcg
     8041 agctgaagcg attgcgcccg gagcacttga aagtattatt gctgagagtg acggcattat
     8101 ggtagcccgc ggtgatttgg ctattgaggt tggtaaccct gcggtaccag ccttacaaaa
     8161 gcgcatgatt cgcttggcct tagaggcaga caagttcacg attacagcaa cccagatgat
     8221 ggagtcgatg atcaatgcac ccgtaccaac gcgcgccgag gtgagcgatg ttgctaacgc
     8281 agtattggat ggtactgatg cagtgatgct ctctgcagaa tctgcgaccg gacagtttcc
     8341 agtaaaaacc attgaacaaa tggcagcgat ttgcattgag gctgaaaaat ccgaagttgt
     8401 gaacttggat accgatttct tggaccaacg ttttgagcgg attgatcaag cgattgctct
     8461 tggcgcatta tttactgcac atcatttaaa tgcaacggcc attgccgcat taaccgattc
     8521 gggttcaacc ccgatttgga tgagtcgcca caatatccgt gtcccaatcg tggcactaac
     8581 gagccgaatt gaaacccagc gtgcgttgag cacttatcgt aatgttaggc cctttagcat
     8641 tgaggcggga gttgaccgtg aatcggcatt gttgggggtt gagaaacgtc taaaagaatt
     8701 aggggtagca aaatcaggcg ataccatcgt cttaacgatt ggtgagccaa tggatcaagc
     8761 gggtggcacg aataccctaa agattgtgaa agttcgctag tcatgaatgc gttgcacagc
     8821 acctcaattc gttctttacc tctaatctct cagggtaagg tacgcgatgt ctatgctgtg
     8881 ggtgatgatc ggctattaat gatcactacc gatcgcattt cagcatttga tgtgattatg
     8941 caagaagcca ttcctgaaaa gggcatggtc ctaaatcaaa tgagtaattt ttggtttgct
     9001 catttggctc atgtgatccc taatcatttg acaggaattg atccaagtac tgtagtggct
     9061 tctaatgaga tcgatcaggt caagggcagg gctgttgttg caaagcgcct caaacctatt
     9121 cttgttgagg cagtagttcg tggttatctg gcggggagtg gctggaagga ttatcaagat
     9181 actggggcgg tttgtgggat tcgtttaccc gctggtctgc aaaatgccca agtcttagca
     9241 gagcctattt ttacaccagc agctaaagcg gaaatgggcc atcatgatga gaacatttcg
     9301 tattcagaag tagaaaaacg gattggtact gagcttgcgc acaagattaa agaaacaagc
     9361 attcgtttgt atcgcgaagc cgctcaatat gctttagaaa aaggcatcat cattgccgat
     9421 acgaagtttg agttcggttt agatgaaact ggacaactgg tcttaatgga tgaaattctg
     9481 acggccgatt catcacgatt ctggcctgcg actacttatc gggtaggaac taacccacca
     9541 tcgtttgata aacaattttt gcgagattgg ttagagtctg ttcgggtcga tggtaagcct
     9601 tgggctaaag cgccccctgc gccccattta ccgcctgaga ttatcgaaaa aactgcagaa
     9661 aaatatcgcg aggcattgcg taggttgacc caatagtgct catgacgtta agagcctaaa
     9721 gactgcgata atatagtttt tcaagccctt ttggagagcc aatggctgat caggttctgg
     9781 taggagttgt gatgggatcc aattcagatt gggataccat gcaatacgct gttcagatat
     9841 tggagcagtt caatattcc
//
LOCUS       DDPQ01000118           22635 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_1015,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000118 DDPQ01000000
VERSION     DDPQ01000118.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 22635)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22635)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22635
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_1015"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1857..1884
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15584..15630
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21107..21152
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6134 a         5545 c         4885 g         5950 t
ORIGIN      
        1 ccctgacgta tataggcgcc catttgcaag ttctccagaa ttgtcatgcg agcaaaaaca
       61 ccgcgtcctt ctggtactaa gcccactcca agctgagcca actcataagc cggtcgaccg
      121 ccaatagagt ctccatcgaa atgaatggag ccggcactcg gctttaacaa tcctgagatt
      181 actttcagag tcgaggtttt tccagctcca ttggcaccaa tcaaggtcac cagctcgcct
      241 cgatcgacgt aaaaattaat tcctttaaca gcaccaatac cgccatacgc cagcttaaga
      301 tcacgcaccg ctaacaagtg ctcactgtga gagcccatca tgccaccact ccccccaagt
      361 acgcacggat cacctcagga tgagctcgaa cctcattggg caggccatta gcaatcacct
      421 tgccataatc cagcacagtt aaatgatcac aaatgttcat caccaaatta acgtcatgct
      481 caatcaataa gatcgtttta ccatcagctc gaatcttcag aatcagatct ttaagctcca
      541 gtttctcact ggcattcata ccggctgctg gctcatctag cgcaagcaga attggttcag
      601 ttgccaatgc tctcgcaatc tcaaggcggc gctgataacc gtatgataaa tttttagcct
      661 tatgctgtgc ataatcttgc aatccaacat acgctaacaa ttgataggat ttatcccgaa
      721 tcgcttgttc ttctttttga ttcgttcgtg ttgccaagat ggacccccat acaccaacat
      781 taaagcgaca atgacatccc accataacat tttctagcgc gctcatttcc ttaaagagac
      841 gaatgttctg aaaggttcgg gctattccgg cttgggtaac ctcagatacc gaacgtggtg
      901 aatacggctt accatttaac tcaaaagatc cgcgatcggc aggatataag ccagtaatta
      961 cattaaagaa tgtggtcttt ccagctccat ttgggccaat caaaccagcg atggtcccat
     1021 cctgaacttg tattccaaca tcatctaaag cctggactcc gccaaagcgt ttagagacat
     1081 ctgatatacg caaaagaaca ctcacgcccg cacccgcttt aattgctctg gcttttgcca
     1141 aatcccattg gggcgataaa gcatgatcaa aataagagct aggccatata acaattgacg
     1201 aataatctcg acgtccataa tgaccgagcc aaataaaaat tgttgaactg gggctgcaat
     1261 acttcgtaat atctccggaa agatcgccaa taacattgcg cctagaatga cgccagggat
     1321 atgtcccatg ccccccaata ccaccatggc caagaccaca atcgactccc atagagtgaa
     1381 tgactctggc gagacaaaac cttgaaaggc tgcaaataag actccagctg ctcctgcaaa
     1441 acttgcacca atggcgaagg ccagtagttt catattgcga gtattaatac ccatcgcctc
     1501 agcagcaatc tcatcttcac ggattgcgat ccaagcacga ccagttcgtg agtgctctaa
     1561 acgcgaacat actaatgcaa ccagaacggc tagtagtagg aagaagtaga acaccaaata
     1621 cagggaagga atctgtaaac caaataaaga aaggggtttg gataagttca atccaaatag
     1681 ttgaatggaa tcaatgttcc caatcccttt gggcccattc gtaaagttga ttggtcgatc
     1741 taaattattt agaaagatac gaatgatttc tccaaacccc aaggtgacaa ttgccaaata
     1801 atcaccgcgt aatttgaggg tcggaaatcc taataagatc ccaaacaatg ctgctannnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnntaacct aaatccaata agccagcaaa gccaatgacc
     1921 acattcaagc ccaatgccag cactacatat aacaaagcaa aatcaagaac gcgtacccag
     1981 taattaccgc cagcagcacc tactacccaa ggtaatagca ataaggtcag ggtactaaat
     2041 acccaaaacc gtttatcccg aagagagtaa agtggtgaca ggtgcttaag cacggtcgga
     2101 caccttctcg cccaagagcc ctcgcggacg aagcactaac accaaaataa gcaccatgaa
     2161 agcaaaaata tcctggtaat ttgatcctaa gaccccgccg gtccaatcgc caatatagcc
     2221 agctcccaaa gcctcaatca ggccaagcaa caaacctcca agcattgccc cttgaatatt
     2281 accgataccc cccagaacgg ccgccgtgaa cgccttaagc cccggaataa atcccatggc
     2341 aaagtgagca ttgccatagt tcgttgccat catgacccct gccaaggcgg cgagtgaacc
     2401 acccaacata aacgtaatag agataacacg attcggatta acgcccatca gggcagcaat
     2461 attgggttgc tctgcggttg cccgcattgc tcgtcccaag cgtgttttat gaacgattag
     2521 taggagtaag cccatcacga tgaaagaact taaaacaatc acaatttctt ttgcggtcac
     2581 tgttgctccg gagccaaata aatcaatagg ggttgaaggc aacaattgtg ggtaagtcaa
     2641 gggattgcgt gaccaaatca tcatggccag agtttgtaat aggaccgata ctccaatcgc
     2701 agaaatgagg ggcgccaaac gcggagcatt gcgtaagggt cgatatgcaa aacgctcaat
     2761 cagaaagctg agtgccgcgc agactgccat tgcccctaat aaaacaatca atagcatggc
     2821 ccaggatggt aatcctagag gtaactgcat gatcaaagtg atcagagtca aggacaccat
     2881 agcgcccacc attaagacct cgccatgggc aaaattaata attcctaaaa tcccatagac
     2941 catggtgtag cccaaagcaa tcaaggcata gatgctccct agcactaagc cattcacaat
     3001 ttgttgcaac agaatatcca tagaccttag tctacaaaga aataagcacc gcagtgcggt
     3061 gcttatttgt tcgttcttac atctttatga tttcaaggac tgacttcttt ttatccttgt
     3121 agtcataaag cgtaatgaca cttgctttta ggtcgccctt atcatcaaag gcaatattac
     3181 cgatcaaacc cttcatctga gtttgtggca tcgccgccaa aattttggca gtttctacag
     3241 aatttgcgcg cttcatagca tcaacaatga catagacagc atcgtaggca aagggtgcat
     3301 agatatcgac atcgatattg aagcgctcct tatatttctt ctgaaactca ggacctgcct
     3361 ccattttcga taaagccatt gctgcttccg agcaaactac attattgatt gcatcacccg
     3421 caagctcgac taacttatca gtacacattc catcgccacc cacaatcttg gctttaatgc
     3481 cgagttcctg agcttgctta gctaaggggc cacccgtggc atccatacca ccatacataa
     3541 tgacatcagg cttccctgct ttgaccttag tcaaaatcgc cttaaagtcg gttgccttgt
     3601 catttgttgc ttcacgggct agtactttga taccggcagc ttttactgtt ttctcaaatt
     3661 catcagcaag ccctttgcca tactgggttg aatcatcaat gatggctacc acttttgcct
     3721 tgatgttatt cgccacataa ttcgctaatg cgggcccctg ttgtgcatcg gttgctacca
     3781 atcgataggt cgtcttgaaa ccttgcttgg tgtaatccgg attagtggcc gatggagaca
     3841 cttgtgtaat accagcatca ctataaatct ttgctgctgg aatactggtt ccagaattca
     3901 agtgcccaac cacagcaacc acttttgcat cgactaattt ttgtgcaacc tgagtggcgg
     3961 tctttggatc tgctgcatcg tcttcaggaa ctaacttcag cactactttt ttaccattaa
     4021 tagtgaggcc tgctttgtta atttcctcga ctgcaagtaa ggctccgttt tcattctgct
     4081 tacccatatg ggcaatggat cccgtaatag gcgcaacgtg cccaatctta atctcattgt
     4141 ctgcaggctt tccacaggct gccaataggg cgatagcgac tcctgacaac agaccatatg
     4201 agaatacttt ttgatacgtt ttcatagcca aaatctccta aggatgatga aataatattt
     4261 aactagggtg agtcaaattc tttggtagcg aaaagataac gtcttcaatc actccgtcta
     4321 aagaacgcac ttgacgcggt ccaaactctt gaatccgcga tacgatcgct tgtgccaaac
     4381 tctctggggc tgaagcacct gcagtaagcc cgatacgctt cttaccagcg aaccattctg
     4441 gcttaagctg ttctggagca tccaccatgt aagcaggtac gccaagtttt tcagctaatt
     4501 ctctcaggcg attcgaattc gaactcgcct tgctacccac gacaatcact aactccactt
     4561 tgggagccat gaacttgacc gcatcttgtc ggttttgggt ggcgtagcaa atatcttgtt
     4621 tcttgggttg aataatttta ggaaatttct tcgtgagtgc attcacaatt tctttagtct
     4681 catccaccga taaggtagtt tgtgtaacaa aggctaaatt ttcattcgcc gagaaagcga
     4741 gtttctccac atcttcaata ctttcaatta aaaaaatgcc cttatccact tggcccatcg
     4801 tgccctcaac ttcaggatgt ccggcatgac caatcataaa taccgtatag ccatccttgc
     4861 acaacttgac aacttctaca tgaaccttgg ttaccaatgg gcaggttgcg tcatacacct
     4921 gcaagccacg attctcggcc tctttacgca cttcttgaga gaccccatgt gcactgtata
     4981 taacaatcga cccacgtgga atttcttcta tctcatccac aaatattgca cccttctcgc
     5041 gcaactcgtc aacaacatat ttgttatgaa caatttcatg gcggacatag atgggtgcgc
     5101 caaaacgatt caatgcttca ttcacgatat tgatggcgcg atcaacgccc gcacaaaacc
     5161 cacgcggctg tgccaacaaa atttcagagg aatcttgtga tgtactggta tttgacatgg
     5221 tttgtttttt acaaaatagc aactatttca gcttcaaaac tgaggtcttt acctgccaag
     5281 gggtggttga aatcgaacca cgcgccttcc tcgttaatcg actgtaatac acctgcatat
     5341 tgtgccccgc ctggagcatt aaactcaaca atatcacctg gcttaaattc tgatacctcg
     5401 tcagtattgc agactaaggt ctccatagag acccactgca ctaactctgg cttgtgctcc
     5461 ccaaagcctt cttttgccga gatcgtggcg gaacgtttct caccaacctt tgcgcccatt
     5521 aatgcttttt caaagcaggg ggcaaattga ccggttccca actgcatagt cgcagggcta
     5581 tcctcaaagg tattgatgta gtcctccccg ttgggcaaaa ccaaacggta attcagggtc
     5641 aaaaaagcag tgggggtaac ggtaagctca ctcatatggc cattgtagcg agagagtccc
     5701 agcatcgccc aatctcttca tggccaaaag cccatcagcc tcgggaaaaa ttacgcctcc
     5761 agggtgcgag ctccctctcc gatgcagaat tactagccat ttttttaagg gcgggcgtga
     5821 agggcaaaag tgccgtttca ctggcacaag atctcttgac acaatttcaa gggattcagg
     5881 ggctagtgaa tgcaaatacc gaagatattt gtaagtgctt tgggatgggg ccatcgaagt
     5941 gggcacaaat tcaagctgcc tatgaacttg ttaggcgctg tctccaagaa agtctatgcg
     6001 ctggggctgt ttttagttcc ctcctccagg tcagagagtt tttacaggcc aaaattggga
     6061 gactacctca tgaggtcttc ttatgtcttt acctagactc cagtaaccga ctcatcgaat
     6121 gccaagagct ttttagaggt tcgattgacc gaacggccat ctaccccaga gaaataatta
     6181 aagagtcctt agcgcgaaac gcaagcagcc tcatcgttgc ccacaaccac cctggcggga
     6241 accctctccc aagccctcaa gatgaattga tcactaaaga ccttcaggag gccctttctt
     6301 gggttgatat tgaacttctc gatcactgca ttgttgcagg gcatggattt ttctcatttt
     6361 cagatgcagg ccttttaaat aggctataaa tgatagtaat tactaactta atataaggtg
     6421 ccctactcac cgcgatgctt tccctagccc taatcgctag atgcctgcta caatctttgt
     6481 ttttcgtaat aaaggagtgt gtcatggcaa aagtttgcca agtcactggg aaaaagccaa
     6541 tggttggcaa taatgtttcc catgcaaaca ataagaccaa acgtcgcttt ttgcctaatt
     6601 tgcaaaatcg tcgcttttgg gttgaatctg aaaatcgttg ggttagcctt cgtttaacga
     6661 atgctggttt acgcatcatc gacaagaacg gaattgattc tgttttggcc gatcttcgtg
     6721 ctcgcggtca agtttaagga atatctgaat ggctaagggc gcaagagaga aaattaagtt
     6781 ggagtccagt gctggtactg gtcacttcta taccactaca aagaacaaaa gaaccaaacc
     6841 agaaaaaatg gagatcatga agtttgatcc aaaggttcgt aagcacgtcg cttacaaaga
     6901 aacaaagatt aagtaagtct taatcttcca ttgaaataaa cccgcttaat gcgggttttt
     6961 tctttactta gtttgagtaa cgtctaaggc gcagcgagaa ctctttaaga gagtttggtc
     7021 cagcctcttc cgctcgatgg caccaagaac gcaactgggt taacagttgc tcgcgggtcg
     7081 cattggagcg cccccaaata gcatggagtt cacgacgcat ctcaatcatc ttgcgcaact
     7141 gaggattggt cttcattaac tcctccagtt tttgtttttc atcagagctt agctttgctt
     7201 cgtccttata aagccaatct cgcgcatctt taaggtgagt ggcaaactct tgcatgttct
     7261 ggaactcatt ggcaatacag cgttgtaagg ttttgctgta gcgggccatg atttcgtaac
     7321 gattggcaat aacagcctca agggtttgat catctgccgg gcgaacctcg gaaagcacag
     7381 gcttgggggg gacctttttc accttagcca ggccaaggga acttagggca cagatataca
     7441 tccaaccaat atcaaactca taccacttgt ttgacagctt tgcgctagtc gcataggtgt
     7501 gatggttgtt atgtaactcc tcgccaccta tcaagattcc ccagggaaca atattggttg
     7561 aagcgtcttc acaatcgtag ttgcgatacc cccagaagtg gccgatacca ttaataacgc
     7621 cggcagcagt aatcggaatc catagcattt gtactgccca aacagtagcg ccaatcccgc
     7681 caaacagaaa tacatcgatg attaacatca gaccaacacc ctgccacgag aacttagagt
     7741 agatattgcg ctccatccaa tcatccgggg tgccatgtcc aaacttatcg agggtctctt
     7801 gcttagcagc ctctatctta taaagttcag cgccacgcaa taaaacagta tcaataccca
     7861 aaatttgtgg gctatgggga tcgtctacgg actcgcattt agcatggtgc ttgcgatgta
     7921 cagatgccca ttctttggta accattccag tggttaacca caaccaaaaa cggaaaaagt
     7981 gggaaggtag tggatgcagg tctagagcac gatgggcttg gcaacgatgt aaaaaaatcg
     8041 tcacacttgc aatagtgata tgggtcatca caagggtgaa aataacaatc tgccaccacg
     8101 cccaatccag atagccattg gccaaccatt gcatcaatga atcatgaaac gaagtgagtc
     8161 cgctaatttc caaacaagtt ctccaaaagg aatctaggct atgaagccct ataccccttt
     8221 attttaaggc tttcggcgta atactgcccc gaagaacccg tcagttccat ggatatgcgg
     8281 ccaaacctgc caccagggct tggcattact gcttcccagg ggtattccat catgtaaaaa
     8341 atgttggcca agggcttctg ctgctggcaa aacctcgaat tctgggtgtt tttctagaaa
     8401 aagttctaca agtccctgat tttcttgatc taaaagactg caggttgcgt agaccaagcg
     8461 accacccggt tttactaatc ggcctgcaga ttccaggatc gctagctgag tttggcctaa
     8521 tttcttgatt tcctcaggag tctgacgcca cttgaggtct ggattacgcc gaatagttcc
     8581 caaaccactg cagggggcat ccaccagcac ccgatcaatc ttgccagcaa ggcgcttaat
     8641 cttgggatcg ttttcactat caatccaaac tggatgaaca tttgataaac cactgcgcgc
     8701 aagacgtggt tttaggtttg ccagtcgtcg ctcggatgta tcaaaggcat acaacctacc
     8761 tgtgcttcgc atcatggcgc ccagagccaa tgtcttgccg cctgcgcctg cacaaaaatc
     8821 taccaccatc tcaccgcgtt ttggggcgag cagatgacac aggatttgac taccctcatc
     8881 ctgcacttca aagaggccat ccttgaacca ggtcgaggtt tgcaaggaag gctttccaaa
     8941 gatgcggatg ccatctaaag aatagggggt tgggattgcc tcaaagcgat tggtgctggc
     9001 attcatttgc gtaagcagat cgtctcgatt aatcttcatg gtattgacac gcaaatcaag
     9061 gggtgcgggt ttcataagag tctctgctaa ttgctcacac tgctctttac caagtgattg
     9121 agttagttca ttccacaacc attctggtag attattttta atcattgggg caagtgcatt
     9181 gcgatcaact tgtccgtaac gctgcaacca ctcaaactca tctgtttgta atacaaaggc
     9241 taaatctgct aaggcgcttt ctaagcgatt gcccgatcca agcccgccct cagagagcgc
     9301 tgaaaccaag cccagcaaag ctaggcgacg tgcctgcggt ccactaccgc tagctgcaaa
     9361 ctgcgagaac tcgtttttac gtcgtaacac tgcgaaggca gcttctgcaa ttaatgctcg
     9421 atctcgattg cctaattttg ggtttgctcg aaagtaacga ctaaccacgc gatccgcggg
     9481 ttgattaaaa agaagtaatt ctggaacgat gtgctctaga tgaaacgcgt gctggggcaa
     9541 agccttagca tgcgaataat ttttttgccc ctgaggattg atgggctcat taggtttagc
     9601 gttctttgct tttgcgtatt tcttttctcc cgccaggcgc ttggaagacc catacgatgc
     9661 ggatgatttt ttagaagtat ttttcatgta aatgagatgt actgtgattc aggtggatgg
     9721 atatgcacag tatttccctc gatctgtaaa cgttggttaa taaaccattc aaccgctaaa
     9781 gggtaaatgt agtgttccat ttttaagacc cgtgctgcca aacgacttgc atcatcatcg
     9841 cgcaatatag ggacctcacc ctgacaaatg atagggcctt catccacacc ggcactcaca
     9901 aaatgaacgg tagccccatg cgtcgtatcg cccgcctcta gagcacgttc atgcgtatgc
     9961 aaaccggggt aacgtggtaa caaggatgga tgaatattga ttatgcgacc ctcataatgc
    10021 ttgataaagg ctggggtgag gattctcata aaaccagcca aaatcaataa atcggctcca
    10081 tagtggtcaa tttcgtgaat aagggcctca tcaaaagcat cgcgactcgc aaaatcacga
    10141 tgattaagca cttgtgtgga aatgccctga gacctagcaa aatcaaggcc ctttgcctct
    10201 ggattattac taataacccc tgtaaatcga acatcccatg actttgcagt agcggtcttg
    10261 aggattgctt ctaaattaga gccccgccca gaaattaagg aaacgattga atgcatgacc
    10321 aacaatataa ttcaaggtta gcctgaaagc gaactgtgaa ggtaattcga cgtttttatt
    10381 catcctctac tcgccctccc tgcgccctca ccattggtaa ttttgatggg gttcatcgag
    10441 gacaccaagc attgctccag caattggtgc aaggggctcg tgcgcgcgag ctatctagct
    10501 gcgtattaac gtttgagcct catccccgcg aattcttcac ccctaatgat gcaccacccc
    10561 gtatccaaaa tttacgcgat aagctactgg cattaaaaag tcttggtttg gactcaatta
    10621 tgattgctga attcaatcag gcatttgcgc tactaagtcc cacagaattt gttacagaaa
    10681 tcctagtaaa gcaactcaac actaaatgga tttttgtggg tgatgatttt tgttatggcg
    10741 ccaaacgtgc tggtaatttt gcaagcctga aggccgctgg tcaacaatat ggatttgagg
    10801 tcaccagtat ctctacggta cttcaggaga acgaaagaat ttcaagttct cttgtgcgcc
    10861 gcgctcttga aaaaggcgac atgctactag ccagtagctt gcttggtcgc ctcttctcta
    10921 tttctggtca tgtcttgtat ggcaaacaat taggacgtca actgggtttc cctacactca
    10981 atctttcagt atcgaccctg cggcgctccc atcaaccagt tactagcggt atttttgtgg
    11041 ctcaggttca tggtcttgcc aaagaagctc tgccagcagt ggctagccta ggcgttcgtc
    11101 catctgtaga gagttctggg gaagtcttat tagagaccca tgtatttaat ttcaatcaat
    11161 cggtctatgg gaaattagtt tgcgtagaat tgctagagcg actgcatgaa gagcggaaat
    11221 accctgatct aaaaacctta caagctgcaa tccaacatga tgcgcaaatg gcgcgcgatt
    11281 attttcataa gaaaaatatg tatgtctgac aaatcctctg cttaccccgt taatctcctt
    11341 gagacctcat tccccatgcg tggagatttg cctaagcgtg agccacaatg ggttgaagag
    11401 tggcaacgca ataaagtgta cgaagcgatc cgctcggctc atgcgggcga acctagtttc
    11461 atccttcacg atggcccccc ttatgccaat ggtgatattc atattggtca cgcagtcaat
    11521 aaaatcctta aggacatgat tgtcaagtca cgttggctaa tgggctttga cgcaatttat
    11581 gtgccaggct gggattgcca tggtatgccc attgagatcc aaatagaaaa acaatttggt
    11641 aagaatttac ccactgccca agtgcaagcc aaggcacgtg agtatgcaaa agaacaggtt
    11701 gcaaaacaga aaaaagattt tcaacgtctt ggtgtcttag gcgagtggga tgagccttat
    11761 ctcactatgg actttcaaaa cgaggctgat gagatccgcg ctctaggaga aatctggaag
    11821 aaggggtatg tattccgggg actaaaacct gttaattggt gctttgattg cggctccgcg
    11881 ctcgctgaag cggaagtaga gtatcaagat aaaaccgatc ctgccgttga tgtgggcttt
    11941 gcctttgata cggcgcagtt aaattcatta gcaaatgctt ttggattaaa agagcttccc
    12001 aataaaccgg gtatggcggt catttggact acaacacctt ggaccttacc tgccaatcaa
    12061 gcgcttaatg cacatcccga tctggaatac gccttggtcg agacgcaaaa gcaaatcctg
    12121 ctacttgctc aagaccgtgt caaagaatgt ctggagcaat atggttttga agggaaagtt
    12181 ctaggtacct gtaaaggtaa ggcccttgag ggtatctctt tctggcaccc tctagccagc
    12241 ctagacccgg ggtataaacg attggcaccc atttacttag cagagtatgt caccttagac
    12301 acgggtaccg gcttagttca ctgtgcgcct gcctacggtg aggaagattt caaatcctgt
    12361 aaaacaaatg gcttagcgga tcaaggcatc ataaatcctg ttatgggtga tggtgtttat
    12421 gcatcatcat tgcccctgtt tgctgggcag catatttgga aagcaaatac caatattgtt
    12481 aaagccctag agcaagcggg tagccttgtt aagaataagt cgtatacgca ctcttatatg
    12541 cattgctggc gtcataaaac gcccattatt tatcgtgcta cctcccagtg gtttgccagt
    12601 atggataaaa aaccatcgga tgggaaagca tctcttcgcg aaacagcact tgctggtatt
    12661 gaaagcactc tcttttatcc agcctggggt aaacaacgtc ttcatagcat gattgcaaac
    12721 cgccccgatt ggaccctatc gcgccaacgg caatggggtg ttcccatggc attcctaatc
    12781 cataaagaaa cgggtgagcc ccatccgcgc actccagaac ttctagaaaa gattgctcaa
    12841 ctcgttgaga agggtggtat cgaggcctgg caagctttag atcttgccga tttaattggt
    12901 gatgaatcta agcattatga aaaaaaccga gataccttag atgtttggtt tgactctggc
    12961 actacgcact ggcacgttat gcggggctca catcgtactg ccctctaccg taaagaaagt
    13021 gaaggtgagg ctggtcgctg ggctgattta tacctagagg gttctgatca acaccgtggc
    13081 tggtttcatt catccttatt aacgggtgcg atgctagatg gcaagcctcc gtataaagca
    13141 cttcttaccc acggcttcac ggttgatggt cagggtcgca agatgagtaa atcggtcggc
    13201 aatgtcattg ctccgcaaca ggtagccgat aaattaggtg ctgagatcat tcgactttgg
    13261 gttgcctcaa ccgattactc cggagagatg tcgatctctg atgagatatt aaaacgtgtg
    13321 gttgagagct atcgtcgtat gcgtaatacc ttacgctttc tgcttgctaa tctcacagac
    13381 tttgatccaa agagtcattc aatgccagta tccgattggc ttgagatgga tcgttacagt
    13441 ctagcgcttg cagcagattt acaaaaaacc attgaggctc attaccgtaa ctatgagttt
    13501 catccagctg tatcgaaaat acttggtttc tgctctgagg atttgggagg cttttattta
    13561 gatatcctga aagatcgcct ttatacaatg gcgcccaact cgccaggtcg tcgctccgca
    13621 caaaatgcac tatttcatat cacgcagcat ttactcaaat ggctatcgcc cttcttaagc
    13681 tttactgctg aagaagcatg gcagtcttat ccccactttg ccaaacagcc caagagcccc
    13741 tcgatcttca ccgagcaatt tgggtcattc ccagatattg ccaaccaaaa tgagctactc
    13801 caaaaatggc aacggattcg tgagattcgt tctgagatta ccaaagccat tgaagtagaa
    13861 cgcgaggcag gaaaaattgg gtcttctttg caagcagaat taagtatcga ggtggcagca
    13921 tccgactttg cactactcaa ctcacttgga gttgatctgc gctttgtcac aatcacctct
    13981 aaagcagaga tcactcaatc taacagtaat gcaatggcta ttactgttaa accaagcacc
    14041 gctcaaaaat gcgcgcgctg ctggcaccat acaagcgatg ttggatcaca ctccgagcac
    14101 cctgacattt gtggtcgctg catcagcaac ctctatggcg agggcgagaa acgccaattt
    14161 gtataactat ggataaatat caaagcccca atggatcaac tcggtggtat ctcgtactag
    14221 ccttgctgat cctcatagtt gaccaaatta ccaaatggtg ggcacaaatc agtctgccca
    14281 tgggtcaagc acttgcggtg actgattttt tgaactggtt tctcatttat aaccctgggg
    14341 ctgcattctc atttttatcc caagcaggag gctggcaacg ctggtttttt accatcgtcg
    14401 ggatccttgc tgctgtggtc attatttggc tcctccaaaa aaatacacac gatcgttcat
    14461 tttgtatcgc cctctcacta attctgggcg gtgctattgg caatgtgctg gatcgccttt
    14521 tgtatggggc tgtagtcgac tttatcgatg tgcactatca aggttggcat tggcctgcat
    14581 ttaatatcgc tgacagtgcc atctctattg gtgctacatt aatcgttatc aacgagatta
    14641 ggcgtgccat tacgcaacgc agctagtctt actgaaagat cggaatggat gcaatggctt
    14701 ccttagcaaa taaaaaaatt gtgttgggga tctctggtgg gatcgctgcc tataaatcag
    14761 cagagctggc tcgtgcctta atacaagaag gcgctcaggt tcaggtggtg atgaccgaag
    14821 ctgcacagca atttattact cccgtaacac tgcaagctct cacggggcaa tcggtataca
    14881 ccaatcaatg ggatggtcgc attgccaata acatggcaca tattgaactc tctcgccaag
    14941 cggatgcaat cctaatcgca ccagcaagtg ccgatttaat ggctaaactt tctttaggtc
    15001 ttgcagacga tcttttgagc acgctatgcc tcgctcgcga ctgcccactt ctgatcgctc
    15061 ctgccatgaa tctacaaatg tgggcccatc ccgcaaccca acgaagtctt gaacgtctta
    15121 acaaagatgg tgttatggtc ttaggtcctg ggagcggtga tcaagcctgc ggagaggtcg
    15181 gcttaggtcg catgctagag ccctccgaaa tctgtgagca attgattgct ttctttcagc
    15241 ctcaggttct caaaaataaa cgggtgctta ttacggctgg ccccaccttt gaggcgattg
    15301 atccagttcg cggcatcacg aaccttagct caggcaagat gggctttgcg gtagcgcaag
    15361 cagctgttga ggctggcgcc caagtgcatc tgattgctgg tccctgcgat ctagctaccc
    15421 ccctcgtcag tagtggaaga attttgcgta ctaatgtcac tagtggcgaa gaaatgtatc
    15481 aagcagtatt agattcggca acctgtgatg tattttttgc tgttgcggcc gtagctgact
    15541 ggacgattgc gaatcgcgcc aaacaaaaga ttaagcgtca agannnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaatccaaac cctattgtgt tggttttgct
    15661 gccgagaccg atcggcttgg taagcattgc aaagaaaagc gggttcgcaa aggtatcccg
    15721 atgatcgttg gcaatattgg gcctgcaacc ttcggcttag atacgaacga gattttagtg
    15781 gtggatgaaa aaggcagtaa aaacttgggg cgagcgagta agctcgatct ggcccgacgt
    15841 ctgattgcga ttgtggcaac ccgtatttcg taaaggatct tcagtgcaag tacttcaagt
    15901 caaaatactc gacgagcgga tgcgctcaca aatgccaagt tacgggaccc ctggtagtgc
    15961 gggattagat ctgcgcgctt gcattgatca atccattgag ttagtccctg gcaaaacaga
    16021 gttaatccca accggtcttg cgatttatat tgaagaccca cgttatgcgg cgatgatctt
    16081 gccacgctca ggacttggtc ataaacacgg aattgtctta ggtaatttgg tgggcttaat
    16141 cgactctgat tatcagggtc cattaatggt gagcgcctgg aatcgcagca caactccatt
    16201 caaactggag ccgatggaac gcttagcgca attagtcatc gttcctgtgc aacaagtgga
    16261 attaaaaatc gtgcctgagt ttgtcagcag cacccgcggc agtggcggat ttggaagtac
    16321 tggacgcgga taaatccgca tccagtcttc attaaatctc ttctaccggt tcagcactgg
    16381 ctttattagc tctacttggt ggagtattcg atgattgaat atcgagctta accttacctt
    16441 catcatcaat atcaaccagc actgatccac cgtgggctaa acgaccaaat agcagctcgt
    16501 cagccaaggc tttgcgtaca gtatcttgaa tgattcgctg cattggtcta gctcccatca
    16561 atgggtcaaa gccatgtttt gctaagtagg cacgtaatgc aggactaaag acagcatcca
    16621 ctttcttctc atgcaactgt tcttcaagtt gcatcaagaa cttatcaact acccgcatga
    16681 tgattgattc atccaaggcc ttgaaggata cgattgcatc taaacggtta cggaactctg
    16741 gggtgaagaa cttcttgata tcagccatct catcaccctg ctcacgggca ttagtaaagc
    16801 ccatggttga cttctgcata gcctcggcac ccgcattagt agtcatgatg atgatgacat
    16861 ttcggaaatc agttttgcgc ccattgttat cggtcaaggt gccatgatcc atcacctgta
    16921 acagaatatt gaaaatatcc ggatgtgctt tttcgatctc atctagcaat aagacgcaat
    16981 ggggtttctt agaaaccgct tcggttaaaa ggccgccttg gtcaaagccc acataacctg
    17041 ggggcgcccc gatcaagcga ctgaccgcat gacgctccat atactcagac atatcaaagc
    17101 gcaacagctc gatacccata atgaatgcaa gctgtttagc aacctctgtc tttccgaccc
    17161 cagtaggtcc agagaacaag aatgagccga tcgggcgatc aaccttaccc aagccagccc
    17221 gggtcatctt aatagcgctg gccaaagcct caatcgcagg atcttgacca aacacgacgc
    17281 tcttgatatc gcgatctagg gtctgtaatt tactgcgatc atccaccgat accgattgcg
    17341 gcggaatacg tgcaatcttt gcaacaattt cctcgatctc ttggcgacta atggtcttct
    17401 tttgctttga cttgggcaag atgcgctgag cagcacctgc ttcatcaatg acatcaatcg
    17461 ctttgtcagg taaatgtcga tcattgatat agcgcgagga tagctcggca gcggcgacca
    17521 aggctgcgct tgcatactta acaccatggt gttcttcgaa acgggatttc aagccacgca
    17581 aaatttgtac tgtctgatcc acagtaggct caaccacatc caccttctgg aaacgtcgag
    17641 atagcgctgc atctttctca aaaatgccac ggtactcggt aaaggtagtt gccccaatgc
    17701 actttaaagt accgcttgat aaagcaggct tcaatagatt actggcatcg agagtacctc
    17761 cagatgctgc gcccgcacca atcaaggtat gaatttcatc gatgaagagg accccatggg
    17821 cgttatcttt taatgattta agaacactct tgagacgttg ctcaaaatcc ccacggtatt
    17881 tagtgccggc aagcaaggcg cccatgtcta aagaatagac cgtggcgttc gccaaaatat
    17941 caggcacctc tccctttgta attctccagg ccagtccttc ggcaatcgca gttttgccaa
    18001 cacccgcctc acccaccaac agtgggttat ttttacgacg acggcataag acctggatca
    18061 cacgctccac ttcactatca cgaccgatca atgggtcgat cttgccttgg cgagcaaggg
    18121 cattaagatt ttgtgtaaat tgatctaagg ggctttcttt gccaccactc gctgcctctt
    18181 cggcatcagg gctggcctca gccgctttgg cgggctcggt attatcttta cgaacgccat
    18241 gactaatgaa gttgactaca tccaaacggg taaccccttg ctgttgcaag aaatacaccg
    18301 catgtgaatc tttctcgcca aagatagcta cgagcacatt ggcgcccgtg acctcttttt
    18361 taccattcga ggttgattgg acatgcatga tggcacgttg aatgacccgc tgaaatccta
    18421 aggttggctg cgtatcgacc tcttcagttc ctgggaccac cggcgtgttg tcattgataa
    18481 aattcttgag ttgtgagcgc aactcagcaa tatttacggc acaagccttc aaaacctcaa
    18541 ccgaggtcgc gttatccagc aatgccgata gaagatgctc aacggtgatg aactcgtggc
    18601 gagctgctct cgcatccaca aaagccatgt gtaagcttac ttctagttct tgagcaatca
    18661 tgcttcctcc atagtgcact gcaaggggtg gcccgcttca cgggaacgtt ctattacttg
    18721 atgtacttta gtggaagcta catcgcgggt aaagataccg catacacctt taccagcaag
    18781 atgcacttgc aacatgattc gcgttgctgt ctcttggtct ttattgaaat actcttgaat
    18841 aaccatcacc acaaactcca taggggtgta gtcatcattc aatagcatga ctttgtacat
    18901 ggcaggcacc ttgactttct cagcttgccg ttccagtaaa gccgtatctt ccttatacgg
    18961 gattactgga tttcctccag gaggtgtttt ggggttttga ctcattagaa ccattctaac
    19021 cacacagaaa aaattcggcc aaaattggtt tttcctttac gaaaatccct gaaaaacaag
    19081 gcatttttgt catattgggg taatttttat aaaaaaaagg gggttttcac gtgttattcg
    19141 gtcatatttc cttgacaccc ccttaaaaag ggcaaacaat cgatcttgtg gtgtctttct
    19201 cgtatgagat ttacctgcat taggcgtgtt gtggtgtgag accaataaat ggtgtttctt
    19261 cgcttcacgg ttgtttttaa agatttttgt aatggagttc gcatggcgac cggagttgtt
    19321 aagtggttta atgatgcaaa aggtttcggg tttattaaac ctgatgatgg tgaagaagag
    19381 ttgtttgcac atttcagtgc aatcacgatg agtggtttta aaaccctcaa agaaggccaa
    19441 aaggtaacgt ttgacattac ccaaggtcca aagggtaagc aagctacaaa tattcaagct
    19501 ggctgatcac ttcctgatcc caaacaaaaa ccccagggtc aaacctgggg tttttctttt
    19561 gtagctaacg cacttacata ttgtcgatca tgacctgacc aaagccggaa caagaaactt
    19621 gtgtcgaacc tggtaataaa cgtgcgaagt catacgttac tttcttcgac aaaattgctt
    19681 tctccataga agaaataatc aagtcagccg cctcagtcca gcctagatga cgcaacatca
    19741 tttctgcaga gaggatctca gaacctggat tgacataatc cttaccggcg tacttaggag
    19801 cagtaccatg ggttgcctca aacatcgcaa tgctatcgga catattggct cccggcgcaa
    19861 taccgatccc accaacttgg gcagctagcg catcagaaat gtaatcgcca tttaggttca
    19921 gggttgcgat cacactgtat tcattcggac gcaataagat ctgttgtaag aacgcatcgg
    19981 caatcacatc cttcacaatg atgtccttac ctgttttggg attcttaaat ttgcaccatg
    20041 ggccgccatc cacaagctgt gcaccaaatt cttttgtggc taactcataa ccccagtcac
    20101 ggaatccacc ctccgtaaat ttcatgatgt tgcctttatg gactaatgtc accgaaggct
    20161 tgtcattatc aatggcgtac tggaaagctt tgcgaaccaa acgctgggtc ccttcgcgtg
    20221 acactggctt gataccaata ctcgaagatt ctgggaaacg aatcttcttc acacccattt
    20281 ccttagtcaa aaagtcaaca atcttcttgg cgccatctgt gcccgcttcc cactcgatac
    20341 cggcataaat atcctcggag ttctctcgga agatcaccat atcggttttt tcaggttcgc
    20401 gaacagggga aggcacgccc ttgaaataac gtaccggacg caaacaaaca tacaaatcga
    20461 gtgactgacg aagggccacg tttaaggatc gaatcccccc acctacaggg gttgttaatg
    20521 gacccttgat cgaaacaaca tattccttaa ctgcttccag ggtttcgtct ggcatccaca
    20581 cgtctggacc atagacctta gtcgatttct caccagcaaa aacctccatc caagcaatct
    20641 tgcgcttacc accgtaggct ttttgaacag cagcatcgac caccttgatc atgacggggg
    20701 taatatccaa tccagtgcca tctccctcga taaatggaat gatgggatta tggggaactt
    20761 tgattgaaaa atcagcattc acggtaattt tttcaccagc gggaaccttg atgtgcttgt
    20821 acattgcgct tttctccagt taacgaattc agtattttcg catgagactt agaagtccct
    20881 gctctaaaat gaactatttt aaatcttcga caaatatggc aaataaatac ttgactgttc
    20941 tagtggcctc ttgcctgatg ggccttacac atgcatcgtt cgcccaaagc caaaaactgg
    21001 ccgtgatcga gctcaaagcg ggtatgtacc gcatagaagc tgaattagca gatacaccag
    21061 tagctcgcca aacgggtctt atgtatcgga cctttatgcc aacgaannnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntccattgc attcctagat gatgatggca
    21181 agattgtgaa catctccgat atggagcccg agacccaaaa taatcactgt ccgcgctctt
    21241 cagttcgcta cgccctcgaa atgaatcaaa aatggtttgc gcaacgcgct attggtcctg
    21301 ggacagtggt tcagggatta cctaaaaagt aatgaggctt ggtttaacca aagtgacaaa
    21361 cgtaatgcac gggttgatcg gcccggatta caaaataagc acctgcctcc acggtccagc
    21421 tttgaccagc tgaaaatgat ttttcagctt cgccattgat gctgacatag gcagttccat
    21481 ctaccacttc catcacttct ttagttgtca aatcaaagcg taaggtgcta ggtaatatca
    21541 ctccaactga tttacgggta ccgtctgcca aggtaacggt atgcgacaca cacttaccat
    21601 cgaagtagac attcgctttt ttaccaactg aaacctgatc aaactgcatg tgttcgctct
    21661 cttatcaaaa taaatgatta attatttagc acgcttgcgc tttgcgattt ctgcaatacg
    21721 catgcgtagg gcatttaact taataaatcc acctgcatcc gcctggttat aagctccgcc
    21781 atcatcatca aaggtcgcaa tattctgatc aaataaagta tttgcagagt cacgcgccaa
    21841 tacggataca gagcctttat acagctttag gcgaacatta ccattgacac tcttttgcgt
    21901 gtgatcaatt aaagtttgca atgcaatccg ctcaggtgac caccaatagc cgttataaat
    21961 aagtgttgca taacgtggca tcagatcatc cttaagatgc gccacttcgc ggtctaacgt
    22021 aatactttca atcgcacgat gcgcttttag taatatcgtg ccgcccggag tctcatagca
    22081 acctcgactc ttcataccaa caaagcggtt ttcaactaaa tcaagacgac caatcccatt
    22141 tgcgccgccc aagcgattaa gttcagccag taactcatgg gctttcatag ccttgccatt
    22201 aatagcgacc gggtcgccat tcttaaactc aatctcaatg acctgagctt tatctggagc
    22261 tttttcagga gagaccgtcc aacgccacat cgcttcttca gcctccgcat tggggtcttc
    22321 taaatgacgg ccctcaaaac taatatgcaa cagattggca tccatcgagt aaggcgcccc
    22381 accttgcttg tgcttcattt caacaggaat accgtgcttc tctgcgtaag ctagtagttt
    22441 ttctcgagag agtagatccc actcacgcca tggggcaatt actttaatgc ccggctcaag
    22501 cgcgtaatag cctaactcaa aacgaacctg atcattaccc ttgcctgtgg ccccgtgcga
    22561 cactgcatca gcgccaactt gacgagcaat ctcaatttgt cgtttggcaa tcaaaggacg
    22621 tgctatcgat gtgcc
//
LOCUS       DDPQ01000119            2996 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_1016,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000119 DDPQ01000000
VERSION     DDPQ01000119.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_1016"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2676..2738
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          823 a          632 c          744 g          732 t
ORIGIN      
        1 ggcaataaac cgatactaag ttgagtaatg tatttaagcc acccagcggt tttgggcaaa
       61 gcaccctttc tcctaaggcc aatccaaagt agcgctgcat tcagacatgc ccccaaacca
      121 accgatagag ctagaccagc atgtgcaaat aaaggcacta gagcgatatt ggctatttga
      181 gtaaagacca atactaataa agcaatcttg accggcgtac gaatatcctg gcgggagtaa
      241 aagccgggcg ctaaaatttt gacaaggatg agcccgatta gaccaacacc ataggcagcg
      301 agagcttgac gagtcatttc gacatctaga gcgttgaact gaccgtagtg atataggaca
      361 gcagagattg gtgtgccaaa cagaaagagc gccaatgcgc atggggctgc tagtaaaaag
      421 gtaagacgta gaccccagat caaaagctcc ccagcatgtg caatgtcttg attagcattc
      481 gccttactca agcttggaag taatacggtt cctagtgcaa caccaagaag tgctgtagga
      541 aactccatca gtcggtctgc ataggatagc cacgacacac ttccagtttg taaatgagag
      601 gcgatattgg tgttaatgat taaagaaatt tgggcgaccg ataccgcaaa tacagcgggc
      661 accattaact taatgacgcg acgcgcatct ggatttcccc aggccgattt aatcgccttg
      721 ggaaaaaaag caatacgcgg cagaagtctt agttttaaaa gcgccggtat ttgaatgccg
      781 agttgcaaaa tgcctccaat aatgacgccg gcactcaggg cgtaaatggg ttgctctaag
      841 tgaggggcta ataaaacagc agctccaata aggcaaaggt tcaggagaac gggggtgaat
      901 gcgggcacgg caaagcgtcc aaaggtgttc agaattccgg ccgataggga tactaaggaa
      961 atgagtccaa tgtatgggaa catgatttgg gtcatcaaaa tggtggtgtc gtaagcgccc
     1021 ccacctttga gcccggcagc aataataaaa actagaatag gcgcgccaat cactcccatt
     1081 agaacggtta ggcctaaggc ccaaaacagg agggtggcga tggcgtccac tagaccctga
     1141 gttttattgc gatccccttg attggagatc tctcccagaa tggggacaaa agcttgagaa
     1201 aaagcgcctt cggcaaagag cctacggagc aaattaggca gccggaaggc cacattaaag
     1261 gcatccgtcc actcggaagc cccaaagctc ctggcaatga gggtctcgcg aaccaatccc
     1321 gtaatgcggg atagcatggt caaggagctg actttggctg cggcagaaag caggttcatg
     1381 ggctgatttt cccttatttg ggggtggggt gggcattttg gcctctttcg ggtagaatgt
     1441 caggttttcg agagccgtat tgttgcggca ttgcaaagac agtgaacgca ggcttttggc
     1501 aggtttttac ttttaaaagt ttgatttcat tctgattatt tgatttagat aggttaattt
     1561 aataatggct aataccgcac aagcacgcaa gcgcgctcgt caatctgtnn ggtgacaagg
     1621 atgtagcgaa gaaagtattt attgcaacac agtcaacgat tgacacaatc accgataaaa
     1681 agattgctca caaaaatact gccgctcgtc agaagtcacg actttctgca gccattaagg
     1741 cgatggccta atcgtttttt attaggcctt tagcgggcct aaggtagttt agattttctg
     1801 acccgctctt agatcgagcg ggtttttgtt tcatggagcg ttcttttgca agatatgccg
     1861 cataaaactt ctcaaacaac tcaagatgct catttggctg agctaggtac cggccgttca
     1921 ttggctaagc ctcaggtgcc cgggcagctg aggcactatc tccagttctc agactttagt
     1981 cgggatgagt atgagtattt atttgctagg gcagcgtggt tgaaggagcg cttcaaacgc
     2041 tatgagactt ggcatcccct gcacgatcgc accttggcaa tgatcttcga aaaacattcc
     2101 acacgaactc gtttatcgtt tgaggcaggt gttcaccaat tgggcggaca tgcggtttat
     2161 atgaatacaa gggatacaca gcttggtcgg ggtgagccta ttgaggatgc cgcacaagtc
     2221 atttccagaa tgaccgacat catcatgatt cggacctttg agcaagatac cattgagcgc
     2281 tttagtgcca actcacgtgt cccagtcatc aatgggctaa ccaatcaata tcatccttgc
     2341 caggtaatgg ccgatatctt cacatttgtg gaagcccgtg gaccaattca aggcaagacc
     2401 gttgcttggg tcggcgatgc aaacaatatg gcatatacct ggatacaggc ggctgagaaa
     2461 ttagatttcg agtttcggtt ctctgcgcct gagggctatc agcttgagcg atcgcgcctg
     2521 aatagctcgg ccttaaatca ccttgtagta tgtgccgacc cgaaagatgc ttgcaaaggt
     2581 gctcatttgg tttcaacaga cgtctggacc agcatgggtt ttgaggatga aaatacttcg
     2641 cgtatggctg ctttcaaaaa ctggatggtt tcagannnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg acctcaaagc atagtttggg
     2761 aagaagccga gaatcgcctg catgttcaaa aagctctaat ggagtttttg ctctgtgggc
     2821 gcctataaaa ttatggttgc tacatagcgt atgtgtttta tctttttgat tgaagttctg
     2881 taaaaaacat agaaataaat tatgtccgac attaaaaaag cagtattggc ctattctggt
     2941 ggtttagata ccagtgttat tttgaagtgg cttcaagaca catatgcttg tgagat
//
LOCUS       DDPQ01000120           21336 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11998,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000120 DDPQ01000000
VERSION     DDPQ01000120.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 21336)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21336)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21336
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11998"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    6830..6878
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7820..7883
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11696..11738
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12830..12883
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15929..15959
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21081..21187
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5757 a         4647 c         5108 g         5476 t
ORIGIN      
        1 atgtgagata tagccaacag gcttatttaa caaaagggtg agacgttgct cttgttgacg
       61 tttagcccct gctcgaagtg ctatggtttg atgtcggtaa gctctagtac caagctcatg
      121 tataggctca ccatctacgg tcaccaaacc ctgttcaata taagaatcag cttctctgcg
      181 cgaacaaaga ccgagctcag acagtagttt agagaccctg attttttcca ctatgcactc
      241 atttacgcag ctgtgcttga cgacgaataa tctcagcctc aatctttttt aaagtaaggt
      301 cattgggatt ttgccaatta ccaccagatt gattcaccat ataaacaagg gaggcggcaa
      361 aatctactac gcttagatca ggtttgccgc cttttgcagg cattccacgc acgcccacat
      421 acccatgagc ggttagctgc acttgtcctt ccttaatgag gggagcccat ttagccttat
      481 caccaagctt tggagcacct gcaaccccag cagaatgaca taccgcacaa acagttttat
      541 aggtattttc tgcagggttt gcccagctgt tggacgaaaa ataaatcgag gataaaaaaa
      601 cagataaaac aatcaattta atttttttca ttcacactca ccttgctccg gtagatattt
      661 aatccagtaa gatgaataaa acaaccaaaa ttgagtctct atgaatcgtt ttattcgttc
      721 cattcttatt ttaagtctta ttggcgtagt ttttgtctcc ggatatacct atttcatgct
      781 gaattggagc tatgcggagg gtgagcgagc tggctatgta cagaagtttt caaagcgcgg
      841 ttggctttgt aaaacctggg aaggagagct cgcaatggtc tcaatgcccg gcaccatgtc
      901 agagaaattc ttcttttcgg tgcgcgaaga tgcggtcgct aacaaaataa acgctaatat
      961 ggggcgaaaa gttgccctgc agtatgaaca acatgttggt ttaccaagca gctgttttgg
     1021 agagactcaa tattttgtta aagatttatt ggttctagaa tagtcttaag ataaaaactt
     1081 agactccaat ttttttatca agaatattta tatttgtaat attttgtgtt tacaattgca
     1141 ttggcgctaa aagaaagcgc caaacaatga acaatcaaca aggagcttca cttgaataaa
     1201 gcagaactaa tcgcagcgat tgctgacgat gccgaaattt ctaaagccaa agctgagtat
     1261 gcattgaact ctgctatcga gcacatcatt aaggcagtaa ccaagggtga ttcagttcaa
     1321 ttaatcgggt ttggaacatt tgcctcagga aagcgcgctg cacggatggg ccgcaatcca
     1381 aaaacagggg agccacttaa aatcgccgcc tcaaagaccg tgaagttttc tgctggtaag
     1441 gcatttaaag actctgtcaa taagcgtaag aagtaattcg ctgcttttat ataaacccag
     1501 ctgcggctgg gttttttctt gcctgaacag ttcgttaact ttgcaccaag cgtcgatggc
     1561 gatgaatgct catgaggatg cctgccccaa agccaagtgt tacaagggct gtgccgccat
     1621 agctgagaag cggtagaggc actccaacaa ctggcagtag gccggtcacc atgccaatgt
     1681 tgacaaaggc gtaggtaaaa aagatgaggc taatagcgcc gccaagcaaa cgtgtaaaga
     1741 gggttggagc ccccatcgaa atggtaagac cgcgcttgat caaggcaaaa aagagcagaa
     1801 ttaaaacaag gttgcccaaa aacccaaact cttctgcaaa aaccgcaaag acaaaatcag
     1861 tatgtttctc gggaataaat tcgaggtgtg attgcgttcc ttcgcgccag cctttaccaa
     1921 aaaatcctcc agagccaacc gcaatcattg cttgaatcgt atgaaatcca cggcccaagg
     1981 gatcattaga gggatctaat agagtacaga cccgattttt ttgatactct cgaataaggg
     2041 gccagctgac atcaggtgcg caaatgctgc tactaaaaac aataagcata agaatggcga
     2101 tgccgcccgc tgcaataata ggcacaatcc agcgccatgg caaaccggct aagataatca
     2161 cataaagccc agcagcaaga actaaaaggg ctgttcctaa atcaggctgg cgggcaatta
     2221 aaaaaactgg taaggctaga atgagtccag caacaagata gtcaagggta gagcgaatac
     2281 cctcacgttt ttgaaaatac caagccaaca ttaatggcac agctattttc ataatttcgg
     2341 atggttgaat cgtaatgccc aaatttatcc atcgacgagc tccctttttg ataaggccaa
     2401 agatggccac agcaattagc agggcaatgc cgatgccata gacccaaact gccgccatct
     2461 ctaaccactt tggaggaatt tgcgagacga tccacataat gacaaaagca atgaacaaat
     2521 tacggatttg atcaacaatc gcaataggcg tcccttgccc ggctgataaa aatacaataa
     2581 agctaattcc agctaggcca agaaaaataa agccaagttg aggatcaaag ccccgaaagg
     2641 cataaaaaat aatagacctt actttggcta gggcactctt ttccactcgg ggatctcctt
     2701 tggccatttg ccgtcaatat agtaatccag agctttgcgc gcaatgggcg cggcatgttg
     2761 tgctccaaaa ccagagttct caacaatcat ggcaatcgca atttttggct tgtcgtttgg
     2821 tgcgtaggcc acaaataaag catgatctct taaaaactca ggcgtggtgg cgtggttata
     2881 tgttttggaa tttaagctaa aaacttgcgc cgtgccggtc ttgccgcctg caacaaaagg
     2941 agcatttcgg aatgaagaag ccgatgtacc cgatttgtta acctcgacca ttgccttttt
     3001 aataatctca acattttctg gcttaaggtc gatgcgataa ctttctttag gagtagtcaa
     3061 ttctttttgg cgagtgaagg gatcctcaat ggctttggca atatggggtt tcatgaccac
     3121 gccatagttg agcacatttg caagcccatg ggctaactgc aaaattgtga atgcgttata
     3181 gccttgtcca attcccagag aaatcgtttc accgtcaaac catttttgtt gatctggttt
     3241 tttgaaggca gcctttttcc aatcagtcga tggcaaaact ccacggacct cgccatctaa
     3301 atcaatccca gtgatttgac caaaaccaag cggcttcata aagtcatgca tcatgttgac
     3361 gcccatatca cgtgccaaga ggtaatagta cgtatcacaa gattccacga tggacttttc
     3421 catattgacc atgccatggc cgccaacctt atcatcccga aaggtgtgat tgccaaatgt
     3481 aaagaatccg ggatcagaaa tggagtcttc gggcgctcgc ttattaagtt caagagctgc
     3541 taatgccata aaaggcttat aggttgatgc tggtggatag atgcctttga gggggcggtt
     3601 atacagtggt ttttccttag actcgtttaa ctctttccag gtcgttgcat caatgccttc
     3661 tacaaaatca ttaggattga aattgggctt tgacacaaat gccaatacgt cacccgtttc
     3721 tggctcgatc gctacaaatg cacctcgccg ttttccatag agctcctcaa ccaaggcttg
     3781 taatttgata tcaatcgaga ggatgacatt tttccctggg atggacggtt tactagacag
     3841 ggtccgcacc ggtttgccgc cagccgtaat ctcgacctca tcgtaaccag gaacgccccg
     3901 caaaaccctt tcataggttt gttcaatacc aatcttgcca acatactgaa tccctggcaa
     3961 gaaggcgctt tgcagtgcat aggggtcatc gctgcccttg gtgttctcaa gttcggtttg
     4021 catccgctct ttatctttaa cagacactcg gcctatgtaa cctatgagat gtgaggccaa
     4081 ttcgttatag gggtattcac gaaaattacg ggcactaaca tccacaccag gaaagcgata
     4141 gcgatttgct aagaaacgtg ccatttcgtt ttcggtcaac atcgagcgca aaggaaaggt
     4201 gcccatattg cgtgagtcct caagcgctct cttgaaatta cggcgatcgc gcgggccgat
     4261 ttggataatc tcgcccagtt gatcgatgag ggcatctgga ttgcctttaa tttgtgatgc
     4321 atccacctct aaagtcagcg cagagtaatt gcgcccaata acaataccgt tgcgatcaat
     4381 caataatccc cgattagcag ggactggaac aatggcgatg cggttgcttt ctgctagcgt
     4441 agagtatttg ttgtgactaa cgacctgtag ccaaaagaga cgaaaaatta ataggccaaa
     4501 acagattgtg acgaatagga tggcaatgcg cactcgctca tgaaaagaat ataaatctgg
     4561 ctttttaagc gagggcataa aaatctatag cggtcgatta gggtcaacat caatgggccg
     4621 acgctgtggg aaaagcaaca agttggttgc tagtggccat agcattgcct caatcattgc
     4681 ttgcacaata gctgttaggc cccaccagtg aatttgttcg ctaagcgccc aattaaccaa
     4741 aacaggaaac agagaaacta ataaaaagat gggaaagacg ttaagaattt gcgagaacga
     4801 agttagggcg gtaattcggt tatgccacgc aatcgccaaa taagaaacca aagaatagct
     4861 aaatgcatgg acgcccagaa gggagccgct atgaatgtcc attaccagac caaataaaaa
     4921 agcagtcaca atgccaacat agcgatgttg atgaacattc caaaacacaa tacaaataat
     4981 tagtaagtct ggaacccatg catggtttcc aaaaggtaaa aaattgagaa gtagtgcgag
     5041 gaataggctg agatagataa atgatggctt aaccgacctc agaatatagc cgctttgata
     5101 atcaatcatg gctgaccttt tggtctagtt tggcgcctac cgggtgccgg tgcagtagcg
     5161 ggccccccgg aaccaatcgc aggctttgca tcccattgcg gattgtaaat aagcgccaaa
     5221 gcctgcagat ggcgattaac ctccgcgatc ggactacaaa atacattgga tgcattttgc
     5281 tcagcattgc gttcaatttt ggtaatcaca gcgactgcaa atcctggtgg gtatattcca
     5341 tctaccccag aggttaatag aatatcgccc acttctaggt cgctggctac aggaaggtag
     5401 cggagttgta ataagttacc gcgcccagcc ccaacaaccg ctgcacgcaa accattgcgc
     5461 gcaactaaaa ctggcacagc aaaatcccaa tcttctaata aagatacttc agcggaatta
     5521 tcaaaaaggc gaacaacttg tcccaaaata ccagcatcgt ttgcgatggg ggagccaaga
     5581 tttaggccat ggttactgcc acgattaatg acgactcgtt gagaaattgg attgggtgga
     5641 ttaaaaagaa tctcaacagg taccgtagtg aaattagtat ttttctgaag atctaagaga
     5701 tttctgagat tttgattttc aacactaagg agcgctgctt gattggctag aagagacagt
     5761 tcggtttggc gagttttcag agaaagattt tctttctcaa gcgcagagcg agttgtgaaa
     5821 tagtcgtcac cgaaagaaaa caaatcagag ggaagaagca ttaagttttc aagaggacgc
     5881 aaaacataac ttacttgatt gcgaataaag cctaaggact tgacttgaaa atcaatgacc
     5941 atgagagcca acccaattac taaacaagca actaacttgg caagtgcagg tatgccctgt
     6001 ttaaaaagtg ggggggtact attttgcacg accctgctcg gctattagcc gcctactctt
     6061 gggaaaacac tccaccaagc ttatccatac gctcaagggc aatgccacaa ccccgtgcga
     6121 cacaggttag aggatcctct gcaacgtgaa tgggcaaacc agtttcttcc atgagcaggc
     6181 gatcaagatc ccttaacaag gcgccaccac cagtaagcat taaacctcgt tctgcaatgt
     6241 ccgatgccag ctctggaggg gtttgctcca gagctgcttt gactgctgtc acaatttgat
     6301 tcagtgggtc agttagggcc tcaaggattt cattgctcgt taccgtaaag ctgcgtggaa
     6361 taccttctga aatatttcgt cccttgatct ccaattcacg aatctcggct ccaggaaatg
     6421 cagaaccaat cgctttttta accgcctcgg cagtttgttc gccaatgagc atgccgtagt
     6481 tgcggcgaat gtagttggta atcgcctcat caaacttatc accgccgaca cgaacagacc
     6541 ctttgtaaac aacgccacct aaagacatca cacccacttc agttgtgcca ccgccgatat
     6601 caaccaccat cgaaccagcg gcttcagaaa ctggaagacc cgcaccaata gcggcggcca
     6661 tgggctcttc aattaaaaat acttgtgaag cgccggcacc caatgccgac tcgcgaatag
     6721 cgcgacgctc aacttgggtt gagccacatg gaacgcaaat gataattcgg gggctgggtc
     6781 taaagagttt gctctcgtga accatcttga taaattgctt gagcatttgn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ctcgcgccct actgccaaaa
     6901 ttgttttttt gttattgggc ccaccttctt ggcggattgc tacaacggag ggctcatcaa
     6961 ggacaattcc ccgatcacgc atataaataa gggtgtttgc agtacctagg tcaatggcta
     7021 ggtcattgga aaaatagcta cgaaagagac caaacataat gtaagtggga aaatagaatt
     7081 attaatttga acgaagatga aatgatactc tagcacccca tggatataaa tgaagttcac
     7141 cgtatagcga agctatcccg ccttgaatta agcccttcag aggccgaggc ggttttgcct
     7201 cagttgaaag ccatttttgc cttggtagag gagatgcagg cggtcgatac aagcgcagta
     7261 gaccccctgc cacacccaat tttgttcatc caggatttag cccagccttt acgtcctgat
     7321 gcagttaccg aaatggatgc gcgtgagagc aatatggcca atgccccagc gcaagataga
     7381 ggctattttt tagtgcccag ggtgattgaa tgagctggca tcaaaagacc ctcgcccaat
     7441 tatccaaagc attgattaat aaggatattt caagtttaga gctgagcaat tactacctga
     7501 atcggatgga gctcaggaag gatttgaacg cttatttgga tatccagcca gaattaactt
     7561 tggctcaggc aaggcaggcc gataccttgc gtacccaagg caaggccggc ccccttaccg
     7621 gaatcccaat cgcccataaa gatgtctttg ttagtaaagg ctgggccaca actgcagcct
     7681 caaaaatcct cagaggctat atcagccctt ttgatgcagc tatagtcgag gcccttggca
     7741 tgccgtgtga acgccacccc caagctgctg ggatggtttg tcttggaaag accaatatgg
     7801 atgagtttgc gatgggctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnncgggagg ctccgccgtg gcggtagcgg caggcttggc
     7921 tcccattgca acaggaacag atacaggcgg ctcgatacgt cagcccgctg ctttctgtgg
     7981 actgacagga attaagccaa catatgggcg ggtctcgcgc tacggcatga ttgcctatgc
     8041 gtcctcattg gatcaggcgg gccctatcgg aaagacagct gaggactgcg ccttgttatt
     8101 aagcgcaatg tcttttcatg attcgcgtga ctcaacgtct ttatcagata gtggcgaaga
     8161 ctattcacgc tgtttaaacg ccacttggtc tgacaaagat caaagcaagc ccttggcagg
     8221 attacgggtt ggtttgccta aagagtttta tgccgatggt ttatctcccg aggttgccaa
     8281 agctctattg ttagcaaaag aggcactgca tactctgggc gcaagtgtgt gcgaggttag
     8341 cttgcccaaa acaaaattat ccattccggt ttattacgtg cttgcacccg ccgaggcgtc
     8401 gagtaatcta agtcggtatg acggggtacg ctatggtcat cgggcaaaag aatattctga
     8461 tttagtacaa atgtaccaac gctcacgcag tgaaggtttt ggcgatgagg ttaagcgccg
     8521 cattttggtt ggcagctatg ttctctcaca tgggtattac gatgcgtact atttacaagc
     8581 tcaaaaaata cggcgcatca ttgctgcgga ttttcaggca gcgtttaatc aatgcgatgt
     8641 gatcttaggg ccggtggcac cggatgtagc ttggggcttt ggcgagaagt cgaaggatcc
     8701 cttgcagatg tatttagagg atatctatac actttccaca aatcttgcgg gtttgcctgc
     8761 gatgagtgtc ccttgcgggt tttcaaagga gggtatgccg attgggatgc aattgatcgg
     8821 taactatttt tctgaagcgc gactattaca agttgcgcac caataccaat tggcaagcga
     8881 ttggcatttg cgaactcctt ctgaggtggt cgcatgaaat gggaagtggt cattggactc
     8941 gaaacacata ctcaactatt gacgcactct aagattttta gtggagcatc tactcagttt
     9001 ggtgccttac ccaattcaca agcttgccca gttgatttag ccctgcctgg tgtgttgcca
     9061 gttctaaatt atcaggcggt ggcgcatgct attcgttttg gtttggcgat tggcgctact
     9121 atcgcaccag cgagtatttt tgcgcgtaag aactatttct atccggattt accaaaaggc
     9181 tatcaaatta gccaatacga gataccggta gtgatcggtg gccaattgga aattatgctt
     9241 ggccaagagg taaaagtggt tcagctcaca cgcgcacatc tggaagaaga tgctggtaaa
     9301 tcagttcatg agaactttac tggacccaat ggcgaaccag ctagtgggat tgatttaaat
     9361 agagcaggta cgccccttct tgagattgtg accgaacccg taatgcgcag cgctgccgag
     9421 gcagtagcct atgccaaggc tttgcatagc ctggtggtat ggcttggagt ttgtgatggc
     9481 aacatgcaag agggatcctt tcgatgcgat gctaacgttt ctgtacgccc ctttggacaa
     9541 aaggagtttg gtacacgttg tgaaattaag aacctaaact cctttcgttt tctggaagaa
     9601 gcaattaatt atgaggtgcg tcgtcaagtc gaattgattg aagatggcgg tacggtcgta
     9661 caagagactc gtttatatga tcctgatcga caagagactc gcagtatgcg gagcaaagaa
     9721 gacgcacacg attatcggta cttcccagac ccagatctgt tgcccttaat catctcccaa
     9781 gagtggattg aggaagtgcg ctcaagcatg cccccattgc cagccacatt gcgcgagcaa
     9841 tggcagacta attttggact gagtgcttat gacgcacagg ttttaagtca agatcgggca
     9901 agcgccgatt tatttacaga actaattgga ttattgggcg ctgcgcaagc aaagttaatt
     9961 tctaatttca tcactggtga gctggcctcg gctctcaatc gatctaattt acaagcgatc
    10021 gatgcgccgc tgaaagccaa gcatcttagc catttactaa gacgcctaat ggacggtacg
    10081 atttccgcca aaattgctaa agatatcttt gcattaatgt gggaagaggc gcttaatgct
    10141 aaagaggtgt cagatgttga tcaaattatt gagagtcggg gcttaaagca aatcaccgat
    10201 agtgctgcat tagaagcatt gattgattca gtcttgcagg ctaatacaaa atcggtagag
    10261 gagtttcgtg caggtaaaga aaaagcattt aatgctttgg tgggacaggt tatgaaagcc
    10321 tcacaaggca aggccaaccc gcagcaatta aatgaattac tgcgccagaa attacaacgt
    10381 tcatagggaa tgggtaatga gtaatcaaga actgagccaa gaagaacaag aagcgttggt
    10441 agcccaatgg ctgttggcct gcccaggatt ttttgagaga cacgctgaag tgttgcagga
    10501 cgttcgtcta aaggatccgc atagcgaccg tgctatctct ttgcaggaac gccaaatgca
    10561 tttattgcgt tcacaaaatc aggccctgaa ccagcgctta aacgagatgt tacgttttgg
    10621 cagtcgcaat gacaaaactc agcaagcgat ggttgcttgg ctacagcaat taatcgaggc
    10681 tcaagaggct gcggcagttg ccgtagcaat tgagttaggt ctatcaagca tctttgaggt
    10741 tgaggtttgt tgtctattgc aagtcgatga aagtttccag gccttattgg atcagccgtt
    10801 ttgccaaatc tatgcagatt gccctgccac ctttaaagaa aaaatagcgc agtctaaaca
    10861 taatgatgca gattggcaaa gcattgccat attggcatta cctctgggca atgaccagtg
    10921 cgcagctttg gtattggcta gctcagaccc agaacgtttt accaaagaca tgggcgcctt
    10981 ctatttaaaa caaattgctg gcttggcggc cgcagcttta agacgagtcg cctggtaaac
    11041 gcgccgacgt gagcgattct tttcctgaaa tagaagcata tttgcgagcg ttgcacgtct
    11101 tgcgacaact gtcgccccat acccttaaag cctatcgaac agacttgttg gtgcttcaag
    11161 aaaatgcaag ggcggaaggg cttaccctga gcaaggtaag caatgcctta attcggcgct
    11221 gggctgcatc cttacatgcc aagggaagat cacctcgaac gattgcgcgc acactatctg
    11281 cgtggcgtgg tttttatgct tggctcgctt tggaaggcga agcccagaaa tctcattcca
    11341 gcttgagcgt caatcctgtc tcggatgtga aggcgcccaa gcgtaacaag ctattaccta
    11401 aagccttatc ggtggagcag gccattgctt tagtagagca tgctgctaag gaagctaaag
    11461 aaaaaaagga ttgggagtcg tatcgagact acgctacgat cgagttgtta tattcctcgg
    11521 gattgcgctt gtctgaactt ttaggaattg atgttgagcc gagccgatct tctgcaggtt
    11581 ggttggattg ggatgctgcg gaggtatcgg tgctcggtaa gggcaataag cgcagaacga
    11641 ttccggttgg aaagccagcg atgcgatctt tacagagttg gcgggagatg cgagcnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tgttttgcag tcctctcatg
    11761 atttacgtgc tgttcaagag atgctaggtc acgcaagtat tgcaagcact caaatttata
    11821 cgtcccttga ctttcagcat ctcgcaaagg cctatgacag cgcacatccg cgcgctagag
    11881 caaaaagctc aggaaagtaa gatgagcatc agatacagag gccaagtgca ttatctgaaa
    11941 gtatcggtaa gatagacccc actaactgtg cataaccatc accccagaga ataaattaaa
    12001 tggccctgat ccctgtaact atcttgaccg gctttttagg aagcggcaag actactttac
    12061 ttaagcatat cttgaccgag caacacggca aaaagattgc tgttatcgaa aatgagtttg
    12121 gcgaagagaa catcgataac gacatcttgg ttcaaagtgc gcaagaacaa attgtgcaaa
    12181 tgagcaatgg ttgtgtatgt tgttctattc gtggggattt ggtagaggca cttaatcagt
    12241 tgtgggaaga gcgtaagagc aaaaagatta acttcgatcg ggttgtgatt gaaaccaccg
    12301 gagtcgcaaa tccaggccct gtagcgcaaa ccttctttat cgatgacgat attgcaaatc
    12361 gttatgtctt ggatgccgtg gtgaccttgg tggatgccaa gtatggccaa aagcaactga
    12421 ccgaacatga ggaggtgcag cgccaagtag gctttgccga tcgtatcttt attactaagc
    12481 cagatcttgc cagcaaagag gaaattacta gccttaaaaa tcgtttgatg catatgaacc
    12541 cgcgggcgcc aattgagatg attacgcgcg gcgttgctcc cttggacgca gtatttgatt
    12601 taaaaggctt caatctcaat gctaagttgg atattgaccc ccatttttta ggggagcatg
    12661 atcacgatca cagtgagtgt ggacatgacc atcatgatca cgagcaccac aatcaccatg
    12721 accatcatgg ccataccgac cgaatacagt cctttgtgtt caagagcgat aagccctttg
    12781 atcatcgtaa attagaggat tttttggggg gggttatctc agtatttgcn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggctcgg gtgctgtaca
    12901 atccgccgcc acggcattaa aacaaatagg cattgaaact tttatagaaa gagtaataat
    12961 gctcgcttaa tgatgtaatt aggggtttag tagttaccct taatgggcag catttttgaa
    13021 gaagtactaa atgcagtatt aaattatttc ggattgggga acaagatgac cactaaagaa
    13081 gccggcacaa aggcaaaaac tgctgagacc agcaagaaag cctctgcttc gtccgccaag
    13141 ggtgcgccat taacagaagc cgagttgctc aagatgtccg ataaagacta tatgagcgca
    13201 gcgcaattag agttctttcg taccaaattg cagaccctca aagaagatat tctgaagcac
    13261 gcaagtgaga caaccgagca cttgcgtgaa aacattttgg ttcccgatcc cgccgaccgt
    13321 gccacgattg aagaagagca tgcattagaa ctccgtactc gagatcgtga gcgtaaattg
    13381 cttaaaaagg ttgaacaggc cttggctcgc attgagtcgg gcgactacgg ctggtgtgag
    13441 gagactggtg agccaatagg cgtgtcccgt ttaattgcgc ggccaaccgc caacttgtcc
    13501 ctagaggctc aagagcgtcg tgaattacgg cagaaattgt ttggtgaata gggtttctat
    13561 tcatcatgac taaagccgta ccgacattag cggatatccc gccactctta aaagctgaga
    13621 ttttggctga ggccttgccg tacattcggc aataccatgg caaaactatc gtcattaagt
    13681 acggtggcaa tgcgatggtt gaagagcgcc taaaagaaag ttttgcgcgc gacgttatct
    13741 tgctgaagtt agtgggcatg aacccagtgg tagttcatgg cggtggccca caaattgatg
    13801 aagctctaaa gaaaattggt aaaaccggca ccttcattca aggaatgcga gttaccgatg
    13861 acgagaccat ggaggtggtg gagtgggtct tgggtggcga ggtgcaacaa gacatcgtga
    13921 tgttaatcaa tcattttggc ggccaagcag ttggcttaac tggtaaagat ggtggcttga
    13981 ttcatgcgaa aaagttattc gtacccaatg atcagaagcc gggcggagcc ccgattgatt
    14041 taggttttgt tggcgagatt gaggcgatca atcccgcagt tgtaaaagct ctacaagacg
    14101 atgcgtttat tccagtgatt tcgccaatcg gctttagtgc agagggtcag gcctataaca
    14161 tcaatgctga tttagtcgca ggcaagatgg cagaaatcct aaaggccgag aaattggtga
    14221 tgatgaccaa tattccaggc gtcatggata aagccggtaa tttgctaacc gatctcacgg
    14281 cccgcgagat tgatgcttta tttgcagatg gcacgatctc cggtggaatg ctgcccaaga
    14341 tttcatcggc actcgatgcc gccaagagtg gcgtgaactc tgtccacatt attgatggtc
    14401 gaattgagca ctcacttttg cttgaaattc tgacggaaca agcttttggt accatgatac
    14461 gttctagata aacaaaaact cctatgaaca caagcagtag ccctcttgat caggaacttg
    14521 gttctgaatt gaacgggggt gaaaccaaga cccgtaaacg tcctcgcccc ggcgagcgtc
    14581 gtttgcagat cttgcagatt ttggcagaaa tgcttcaaaa cccgcgcggt gagcgagtta
    14641 caaccgcagc tttagctgcc aaaattggtg tatccgaagc cgctttgtat cgtcattttg
    14701 caagcaaggc ccaaatgttt gagggcttga ttgcttttat tgagcaaact gtattcggtc
    14761 taattaatca gatcaatcaa aaagaagaat taggcactgc gcaagtgaaa ggcattgtac
    14821 aaatgctctt ggtctttgca gaaaagaacc ctgggatggt acgagtctta ttaggcgatg
    14881 ctttattgca agaagacgat cgtttgcaag agcgtatcaa tcaagtgctt gatcgggtgg
    14941 aggcatcatt aaagcaatca cttcgcatag cgcaaagtca aggtaaagac ggtcaggcgg
    15001 acatagtcag catgcaggca gccttgttaa tgagttttgt gattggtcgt tggcatcgct
    15061 ttgctcgtag cggctttaaa aaattaccga gcgatggtat tgagtcaagc cttcgcattc
    15121 tccttgcagc atgaccgagc tgtatctttc aaaacaaaca attcactttg ttgagccctt
    15181 gccgctacaa agcggtacca gtattgagaa ctatgatttg gtggtcgaga cctatggcca
    15241 attaaatcct aaggccagta atgccatttt gatttgccat gcacttaacg cttcccatca
    15301 tgtcgctggt ataaatccag atgatccaag tgatattggg tggtgggaca acatggtggg
    15361 tcccaacaag ccgattgata ccaatcgatt ttttgtcatt ggcgtaaata atttggggtc
    15421 ctgttttggt tcaacgggcc ccatgagcat taatccaaaa acaaatttac cgtatggcgc
    15481 tagctttcct gtgttaactg tagaggattg ggtcaacgca caagtgcgct tagccgatcg
    15541 tttagggatc aaacagtttg cagccgtgat gggcggaagt ttgggtggca tgcaggctat
    15601 gtcatgggca acacaatacc cagaacggat agcccattgc gtggtgattg cctctactcc
    15661 aaaattaagt gcccagaaca ttgcttttaa tgaggtcgct cgtaatgcca tattgtcgga
    15721 ccccgatttt catggggggg attactacaa gcatggggtt gtgccgaaaa gtggcttacg
    15781 cctcgcgcgc atggtgggtc atatcactta tttatcggat gacgatatgg ccgagaaatt
    15841 tggacgcgat ttacaaaggc ctgcaggcgc tcctgatgaa taccgtttta gttttgatgt
    15901 ggagtttgcg gttgagagtt atttgcgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    15961 gcgcactcga ttattttgac cctgcaaaac gctttggggg cgacttaagt caagccctct
    16021 ctccagcaca agcaaacttc ttggtggtga gtttttcaac ggattggcgt ttctccccag
    16081 atcgaagccg agaaatagta aaggccttac tcgacaacaa ggcaaatgta agttatgcag
    16141 aaatagatgc gccacatggg catgatgctt ttttattaga tgacgcgcgt tatcacaatt
    16201 tagtacgcgc ttattttgca aaagtggcgg aggatcgatg caattaagag cggatttcga
    16261 tgcgattgct aattgggtta ccccgaattc ctcattattg gatttgggtt gtggtgacgg
    16321 atccttttta gatttcattc gcacaaagcg ccatgtgaag acctatggcg tcgaaatcat
    16381 ggatgcttca gttctggcct gcataaagaa aggccttaat gttattcagc aagatttaga
    16441 aggcggtcta gccttgtttg aagatcaaag ttttgatacc gtggtcttgt cccagacctt
    16501 acaaactatc catcaaaccg aacgtatctt gcgggaggtc gtgcgggttg gtgagcaatg
    16561 cgtagtgtcc ttcccaaact ttggccattg gtcccatcgc tttgcagtgg ccctcggcag
    16621 aatgcctgta tcgaagtctt tgccgtatga gtggtttaat acgccgaacg ttcgggttct
    16681 gaccattgct gactttgagg cccttgcaca taaattaggg attgagatat tgggccgggt
    16741 tgtattacac gaaggtaaga ctgtgcagtg ggcacctaat ctattaggca gcttggccat
    16801 ctatcgcgtg cgtggtgctt gataacgttc attcgtgggg taaagactta cgggtctatt
    16861 tagagtggcc cagcgttcgc atgtttttct tggggttctc cgcgggcctc ccgctattac
    16921 tggtattggg tacccttggg ttttggttac gcgaagcggg aatagaccgc agcacgattg
    16981 gttatttatc gtgggttgcc ttgttctacg cgggtaaatg gttatgggcg ccattagtcg
    17041 atcgtgtacc cattctattt ttgaccaagc gactgggccg gcgaagaagc tggctattgt
    17101 ttgcacaggc cttaattatt tttgggttga ttggtatggc aacaaccgat ccaaaacaga
    17161 acttagagcc catcgtttgg tttgcactct tggttgcatt tgggtcggca acgcaagata
    17221 ttgctttgga cgcttttcgg attgagtcgg caaaagcaca ataccaagcg gcactagcag
    17281 ccagttatca aacaggttat cgcttggcga tgatttgggc aggtgccggg gccttatggt
    17341 tggcagcccg agcagaaaca acaacagggg tttatctcgc cgaggcttgg caatttgcgt
    17401 acttatgtat ggccttatca attggtgtgg gggttgtcac caccctgcta agtccggagc
    17461 ctcagtcagt tttgttacca aaagctcgtt ctgcagggca gtggttgcag cagaccttaa
    17521 tacagccctt tgcggacttt attggccgtt atcggtggca agcattttta attctgggtc
    17581 taatcgccat ttaccgaatt agtgatgtgg tcatgggtat tatggctaac cccttttatg
    17641 ttgacatggg ctataccaag gatgaggtgg cggctatcac caaagtgttt ggcgtgatca
    17701 tgaccctgct gggagctttc ttgggcggtc tgttgacaat gcgttttggg gtgatgcgag
    17761 ttttattttt gggaggtgtt ttatccgcag ccagtaactt gctatttgca tggttaagca
    17821 ctcgcggtca tgatctaaat ggtttggttt gggtaatctc tgccgataat ttgagttcag
    17881 ggatcgcgtc cgcagcattt attgcctacc tatcgtccct aacgaacgtg cagtattcgg
    17941 caacgcaata tgcgttattc agttcgatga tgctgctctt gccaaaatgg tttgcaggct
    18001 tttcgggagt gtacgttgat acttttggct acgaacattt ttttatagca acctcgctaa
    18061 ttggaattcc agtactgatc ttaattacac ttgctatccg ttttgagaaa tcaaactcgc
    18121 cttagagttt ccagtcgtac tcaatgatga gcggggcgtg atcagaaaaa cgttgctctt
    18181 tataaacccc tgcttttctg gcagcctgag cgagttcagg cgtggcaatt tgatagtcaa
    18241 tgcgccagcc cacattcttt gcataggcct gaccgcgttg actccaccag gtataacaag
    18301 tatcactagc atccggctct aattgccgat acacatccac atagcctagt tctgagaata
    18361 gatttgtgag ccaagcacgc tcttcgggta agaaccccga gttctttaga ttacctttcc
    18421 agttctttaa atctatttcg tgatgggcaa tattgacatc accacacaac agaatttggc
    18481 gcccactctt tttgagttgg attagatgcg gcaagaaagc atccaagaaa cgaaacttag
    18541 cctcttggcg ctctggggag ctcgagcctg agggcatata cacagagatc acagagagct
    18601 gccctatctg cacttcaaca tagcgaccct ccgcatcaaa ctcaggattg ccaaatccca
    18661 attgcacatg cttgaccgga tggggtgtga agatgcccga accgctgtac cctttttttt
    18721 gggcatgatg aaagtaagca tgcaggcctg gcggagaaag gatttcaggg ccaaggtcgg
    18781 cctgttgtgc tttaagttct tgtacgcaga taaaatcagc agattgatcg tttagccacc
    18841 ccataaaacc ttttttggcg gcggagcgaa ttccattgag attggcggaa atgatgcgta
    18901 acataaagac ctatgaattt aaatcctgag caagagcagt ttgactttat ccgctttgca
    18961 ctggaagcaa aggttttgtc atttggagag tttaaaacga aggcggggcg gctatcgcct
    19021 tatttcttca atgccggact tttttatgat ggagctcgcc tcgggcgttt gggagagttc
    19081 tatgcccgtg ctctcattca atcgggtata agttttgata tgttgtacgg cccagcttat
    19141 aaaggcatta ccttggcggc tagtaccgcg attgctttgg ctgaacaaaa tcagaacaca
    19201 agctttgcat ataaccgcaa ggaagctaag gatcatggtg agggtggcaa tactgtcggg
    19261 gcaccattgg caggacgtgt ggtcattatt gatgatgtaa tttcagcggg aacctcggtt
    19321 cgcgagtcgg ttgatctgat tagggctgct ggagctatgc cctgtgctgt tttaatagca
    19381 ctcgatcgaa tggagaaatc tggtaatgcg gtcgacgtag gtgaatactc tgcggtacaa
    19441 agcgtttatc aagaatttgg cttacctgtg atcgctattg cagatcttaa gagtttgatg
    19501 gcatatttac agcaatcaaa ggaccctaat cttcagcaat acctaccggc agttcaagac
    19561 taccgcgctc gttatggggt ttagcttaga agatatcttt tagagagtat ctaaatcaat
    19621 ctcaccgtgg tagacggttt gtgccggacc ggtcatcatc acagaaggcg ccacttgagc
    19681 accaaaacca ttccacgcta tctgaagttc tccgccgcgt gtctgaacac ggactgggga
    19741 gtcaagtaag ccacgccgaa tccctgagac aacagccgca caggcaccgg taccgcacga
    19801 cagggtttca ccagcaccgc gttcataaac acgtaattta atttcatggc gattaattat
    19861 ttgcataaag cctgcattaa cgcgcttttg aaaagctgga tgcgattcaa tcaacggccc
    19921 atcaattaat acgggagcgc tatcaagatc ctcaacaatt tggaccgcat gaggattgcc
    19981 catagatacc acagcaatcc aatcagcatg agcgccacta ttttgggaaa gtggcagcgc
    20041 atataaagtc tcatgcattt cttgcttatt ggtcagcccc gaggggtcaa acggaatatc
    20101 agtaggatta aaaattggct gacccatatc aacctcaact tgatcatcgt gaagtcgctt
    20161 gagcgtgaga acagtatgag ccacttccac ttgaatctca ttcttggtgc taagtccctg
    20221 ttcgtgaaca aagcgtacaa agcaacgcga gccatttcca cattgctcaa cctcgccgcc
    20281 atccgcatta aaaatgcggt acttaaagtc aacccctggc ttactacttt tttcaaccaa
    20341 caagatctga tcggcgccaa cgcccaattg gcgatgagca agtctgtgcc agagtgagga
    20401 atcgagttga ctaagatcgt gcgttgtgcc atcaatcaca atgaagtcat tgccagcacc
    20461 atgcattttg gtaaaacgta gtttgcgcat agttggataa ttgcaatcaa agcctaatca
    20521 tacaagtttg cctctccagg cggacgatat ttaaagcgct tgtgtaccca gtaatactca
    20581 gctggccgtt tacgaatctc atcctcaaag cagcgattaa gacgtgctgt atcggccaca
    20641 ggatcatcgc ttggaaagtt ctcaagagca cgctgaatgc ggcagatgta tttactgcgg
    20701 tcagcattta aggtcgtgat catcaggcag acctcagcat tgcaggccct ggctaagcgt
    20761 gagattgcag taatggtatt ggtctgaatc ccaaagaaag gaacaaatac agagtcttta
    20821 gggcctaggt caatatctgg agcaataaaa ataaaattac ctgatttaat ttctcgaatc
    20881 agctccaata ggcggctttg tctagcaata gacttaccgc caaagcgatt gcgccactca
    20941 ataatttttt gattgaagaa tgggttcttc atattttgat aaaggcccgc ccctcgaggc
    21001 cagttttttt ctgccgccat tgcggataaa gccataaagc caccttcaag accaacaaag
    21061 tgcgggatca caaaaatacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnngga aaacataagc gcagatttgt tagcaccact cgtttgcgtt gatgtggtaa
    21241 gcgtccaaat aaggagccta aggcataacc aatttgtacg gtccaggcgt aaggaacaaa
    21301 agcgaatagc cgcaagatgc ttagagcgat gtaatt
//
LOCUS       DDPQ01000121            2507 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_29285,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000121 DDPQ01000000
VERSION     DDPQ01000121.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_29285"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    627..740
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          652 a          615 c          456 g          668 t
ORIGIN      
        1 gccccgttcg caaggatctc aatctctttg acactgcgtg ttgccgtaaa tgagaaggcc
       61 tcttgagtcg cgcgatccat cggtgaaggg cctttagtcg cgtaggcttc ggttactgct
      121 aatttggcac tagaagctaa atttaagccc tcaaccactc tgccacgggc tatgtaatcc
      181 tgatactgag gcactgcaac cgcaaccaag ataccaatga tggcaacgac caccatcacc
      241 tcaattaaag taaaaccttt ttgatttaca tctttattat ttttaagcat aaaaatcccc
      301 tccttaatga gcttccatca tcggataaaa actcattgca gaggggtagt ttgttgtaat
      361 tagtatctga ttttgttgtc agtcaattaa gtgctagctt ttttactatt gcgcttcact
      421 aactcaccaa cagccatcac tccaatcgca ccagcaatac cagcaatggt cgcgtaatcc
      481 gccaagcctt caccaaattg cttaataagc gcaggatcgg tcatcatcat gccacccgca
      541 atccaaccca agagacccgc accagcagtc acaataagtg ggaaacgatc gatgaggtat
      601 agaacaacct tgctaccagc aacaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn atcaacttga cccgctgcac ctgcaattgc aagaacgtta
      781 tctaagctca ttacgatatc cgcaatgatg atagttttaa tagcagcaaa gagtttgtct
      841 ggggcatcaa gattgtgctc ttcctcatcn nccaatccat aacagtaatg cgccaccaac
      901 taatttcaag aatggtattt gtaataaagt gactgcaaaa gcaactagga tgacgcgtaa
      961 gataatcgcg ccagctgttc cccaaataat gcccttcttt ctttgattgg gatgcaagtt
     1021 gcggcaggct agcgcaatca ctacggcatt atcgccgccc aaaagaagat caatgatgat
     1081 gatttggatc accacagacc agtcaatcat tgctaaaaat tccatgtctt cctctatttg
     1141 ttattgatat tgacccaaaa cacttttata tagaacaatg cctgccatta aaactgaatt
     1201 cttaaattaa atcattgctt taagtaaact cgccatctca gaggggtttt tagtgacctt
     1261 aaatccacac tcctccatga ccgctagttt tgcgtctgcc gtatcagcgc cacctgagat
     1321 caaagcccct gcatggccca tacgctttcc tggaggtgcg gttacccctg caataaagcc
     1381 tacgatgggt tttttcatat tagccttgca ccagcgtgcg gcttcgacct catcgggtcc
     1441 gccaatctca ccaatcatga tgaccgcatc tgtatcaggg tcatcattaa acattctcat
     1501 tacgtcaatg tgcttaagac cattgatggg atcaccacca atacctacag cggttgattg
     1561 acccaaacca attgcagaaa gttggcctac ggcttcatag gtcaaggtgc ctgagcgact
     1621 gacaacgcca atgcggcctt tacgatgaat atggcctggc atgatcccaa tcttaatttc
     1681 atcgggggta ataagtcctg ggcagtttgg gcccagtaac aaagtcttct tgccaccctt
     1741 ggcttccttt tgctgcatct tattacgcac ttccaacata tcgcgaacag gaataccctc
     1801 ggttatacaa atcacaaaat ctaagtcggc ttcaactgct tcccaaattg ctgcggcagc
     1861 tcctgggggt ggtacgtaga taacggatac cgttgcacct gtttctttag cagcttcttt
     1921 tactgtgcca aaaataggga ttccaaaaac agactcgcct gcttttttgg gattaacgcc
     1981 agcaacaaaa caatttttgc cattcgcata ctcttggcat ttctcagtat ggaactgtcc
     2041 ggtcttcccc gtaatacctt gggtaatgac tttcgtattt ttattaatca agatcgacat
     2101 aatgtattcc tctaattggg tgtgatggct tatttacctt gggttgcagc aactactttt
     2161 gtagctgctt cggccataga gtcggcgctg atgattggca atccagagtc cgccaaaatc
     2221 tttttgccta actcctcatt agttcccttc attcgcacga ccaaaggtac cgataggttt
     2281 actgctttac atgcagtcac tacaccttct gcaatcacat cacaacgcat aatgccgcca
     2341 aagatgttta cgaggatagc cttgacgctc ttatttttga gcatgatctt aaatgcctcg
     2401 gttaccttct ccgcagtagc accgccacca acatctaaga aatttgcagg ctgaccacca
     2461 aacaatttaa tggtatccat tgttgccatt gctaaaccag cgccatt
//
LOCUS       DDPQ01000122            6789 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_7084,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000122 DDPQ01000000
VERSION     DDPQ01000122.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_7084"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3477..3508
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3739..3751
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1973 a         1572 c         1626 g         1573 t
ORIGIN      
        1 atgtgttgtg gggtaatact tcatgctaga gtcaagcgtg tggtgtttgg tgcgaccgat
       61 cctaaaactg gaatggctgg tagtgttggt gatttgtttg caatccagca gattaatcat
      121 cagaccgagg tagagggcgg ggtattggca gaccagtgcg gtgagttact cagagagttt
      181 tttaaacagc gacgtacatg aagatccatt taattgctcc ctcgggagcg agcccagata
      241 tgcgtagccc agaaagcgct ttgcattggc ttaagcagca gggcgttatg gtctctaatg
      301 cgcaatgcgc acaaagagta tttgagcgct ttgcaggcac tgatgcagaa cgcctcgatg
      361 agttaaatgc aattgctaat ctagacccca atcaggttgt aatctctgta cgaggcggct
      421 atggattgca tcgactcctc gatcagatta attggaaggc aattgcgatt gccgtcaagc
      481 agggtttgca gatctgtggc catagtgatt tcacgggatt tcagatgggg ctattggcca
      541 agaccggcac gattagcctt gcaggcccaa tgctcaatta cgactttggc ccccttaatg
      601 agtttggcga acccgaggct ccaagccaat ttacctggac tcattttcag agtgcggtag
      661 agaaccgccg atttagtatt gcagtacccg atgcccaacc ttgggcaggt gccgttgatc
      721 tttcccaaaa tattgaaggt atgctttggg gcggtaattt aagcatcatc actactctat
      781 tgggaacgga gtattttcca tcggataaac aaataatggg cggtatcctt tttattgagg
      841 atatcaatga gcatccttat cggattgagc gcatgctttt gcaattgcat gaagcggggg
      901 tgctgggtag gcaacgcgcc atcatcttgg gacatttcaa tcaataccgc ttgtacgaag
      961 tcgatcgggg ctataagcta gatactgcaa ttgatttgat tcgtaaacgt gtgcctaagt
     1021 caatccccat tctgactggt ttgccatttg gtcatattaa agataaatta acacttccag
     1081 tgggagctca agtgaactta caagcaagta ccaaaggttt tgtattggaa ggaaaatggt
     1141 aaaacgtgcc cattcgatta ttctggcagg actctgtttc ttatttgcga gcacagtaat
     1201 ggcccttgag gaaccaaaat actctgtgat tgaaagttcc ccaccctttg aattgcgtca
     1261 gtatgcacct atgattgtgg ccgaggtgta tgtggatggt gatatgagtg atgcgggcag
     1321 taaaggcttt cgtctcattg cgggttatat ctttggcaag aaccaatcca aaaccaagct
     1381 agccgatgac ccccaaggta gtgagaagat tgcgatgacc gtacccgtca cgatggagcc
     1441 aaagacaggg ggtaatgaga agattgcgat gaccgccccc gtgaccatgg agacccaaaa
     1501 gaaaaactcc aatcaatggc gcatgttttt tgtcatgcct agtcaataca cattgactag
     1561 cttaccaact cctactagtc ctgaagttaa gcttagagag atccctcccc agaagaaagc
     1621 ggtgattacc tatagtggct ttaattcaga tgaaaagacg caagagaaag cccaggaact
     1681 gcgcgtctgg atgaaatcta aaaacttgac tcctgctagt gagccgcaac tcgcgcgcta
     1741 caacccacca tggagtattc cattcttgcg gcgtaacgaa gttatgtttg actactaaga
     1801 acccgataag atgccccagc atcctctccc aaagcggttt gtaaggtagg tagtagtttt
     1861 tctagatagg gatgtaaggt ccatacggga ttaatgacaa acatcccgct cgcctgaaga
     1921 cgacgctctg ttggctcatg ggtaattcgg aattctgcat gggtccaggg gcgcccatag
     1981 ttttctgcaa ttgctttgag tcgcttgggt aattgaatgg actcagtacg ctggagtatt
     2041 ggataccaaa tgagataaga gcctgttgca aaacggtcca atgcctcagt taagcaggtc
     2101 tctaataaga aataatcctg cttattttca taggaaggat caataaggat caaacctctt
     2161 cggctcggcg gaggaagcag ggcttttaag ctagtaaaac catcctcttt gcgaacctcg
     2221 atttgtcgat ctaaatgaag ttgcttaata ttttgctcca acaagtcaat atcactcgga
     2281 tggagttcaa acagtcgtag acgatcttga gtacgtagga gcttggccat caagaatggt
     2341 gagccagggt aaaaatcaat tcgattcggc ggattaatac tgttaacaag atcaatgtac
     2401 gcttgaagct cagagtaatt cgtctgagga tcttgattac caataatacg atcaattccg
     2461 gtaagcgcct cttgactgac ggtcgcaaag ccctctcgta atgaatacag tcctgcgccc
     2521 gcatgggtat caactagcag gagggcgcca tccttttctt gcaaataaga aacgctatga
     2581 atcaaaacaa ggtgctttag aacatccgca tgattgccag catgaaaggc atggcgataa
     2641 ctaaacatca gcttgaggct tgttctgacg taagacaaac aagacgagcg taatcaccaa
     2701 aatagcactg ccgatgtatt gcaatatctg attatgcgga aagtctaaga ccgtagtttg
     2761 taaaacatac agcactacaa aacctgcaat agcaatgaca cgagggacca ggttggtggg
     2821 actcaattga gcatctctaa tactgagtgc ccagcgactg gcgattaagc cacaccaaat
     2881 acccaatgca gagcccacca atacatcgcc tggccaatgt gccccaacgg cattacgaga
     2941 gatgccggtg aggattgcta agacaaataa aacaatcaat aaattacgct tgggctgggg
     3001 ggtggaaaag aatagcgcac ttgccaccga aaagaccgtt agggtatgtc ctgaaggcaa
     3061 ggcgtgatgt aacaggggat cgcccagtat atgaaagcta ttttggctaa gaaccccggc
     3121 agggcgaggt aagccaatca gaggcttaag actcaaactg attacggctg caagcgcccc
     3181 agagatcaat gctgcacaga gttgtcgagg cgcatagagc accagcggaa agcacaaagc
     3241 aaaaataccc caaccgttac ccaaaaaggt gaaccaagcc catgcccagt tgggtaacac
     3301 ttgagctgcg tgattaagcc aaagaaagag ctctaactct ttaccgctcg ttattaagat
     3361 gagccataga ctcagaggga tgagtgggat agcccaagcg cgaccaaagt tcatgggctg
     3421 gcggtcttta taaagttcga gtgcgatcgg ccgcagcaat ttgagcagcg agttgannnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tgctaattgg gctgtaatcg gaatcgattt
     3541 gccaagagag ccatataacg ccggggtctc tggaccgaag agaactacag tatgtaaatc
     3601 ggtgaccgaa gagaagtggc ctgggcccga gtcattggta accatcgcat gcgatagggt
     3661 gtatagagct ggaagttcag agaagcgcac gtgacctgca aaattgatgg catgttttac
     3721 cttggcagct aagcgtacnn nnnnnnnnnn ntctgcgggt gagcccgtga tcaaaatgag
     3781 atcttcgggc cagtgctgat gtgttgcaac gatcagctct gagaagcgtt ctggggccca
     3841 acgacgttgc gggagcaaat cactcgcatt cgggttaatt aagatgatgc gttgttgacc
     3901 atacttaaac ggaatcatgg cctgttgggc tagattctca atacggagtt gcaggtgttg
     3961 cttgaccata gggtcaataa ctgcctgcgc aagctcaatc tcttgatcgg tgatggctat
     4021 tttgctaaag ggcctttctt cttcttttgc aaaagctgca tacacgagcg atataaagtt
     4081 cttcgcaata tgaatatgcg ggttgtaatg caccttacgg gttagcatcg tgccacgcca
     4141 taaaccttcc ccatgaaaaa tatcatagcc aacacgcctt ctcgcaccac atagaccagt
     4201 cagaagcgcc gtgaaacgag agaacagttc tagatcaatc actgtatcaa ttcgataaaa
     4261 acgtgccttc actaagaagc gcaaagtatc gaaagctaac ttccacaagc tagaggaatc
     4321 aattgtaaaa atatggttgg gatgaacggt atttaacaaa ccaagactgg cagcattgct
     4381 cttgaatata aggaagtaga tctcggcacc attcgcttgg gctttgcgca tagctggatc
     4441 caccaagatg gcgctcccca tttcggagag ctcaataaag agaagctttt taggatcgcc
     4501 cgaccgtgta aatggcctag aaaggatatc cagtaaggca acaatggggc tagcaatcgc
     4561 acaaagtggc acccctaccc agtgatcaat ggtccgcatt gcattgacgc taattgccat
     4621 tctgctttta tcctttatgt cctaggctat attttatgcc cacgaccgtc taactacgct
     4681 ttagtaattt gtaggcgcct ggcaagacgc acaataaagt aattgcgcca tagctaatgg
     4741 atcccaaaac agttagggag ggattaactc cccagagggc caagacagag gctagggtag
     4801 cttcacgtaa gccccaaccc gagatactaa ttggtaacat gagtaaaaga ctcaaggcgg
     4861 gtaaaccaat catcagggcc tcagtcggta cctcagcacc ataggctttg agacaaaaac
     4921 ctaaagccaa aatattgaag caatgaatgg ataccgcaaa tatcccttga gcaatattgg
     4981 tgggccaggc aaacgccaga cggattccgg gaagagcatg atgcaaatca aagcgcgcta
     5041 aaagtttttc aatccagatc agtagcggtt tatagctcag agcaaaagca agtgcgcacc
     5101 cagcacccaa catcactagc agaatcaagt aacccaattc agtgccccag gtgcctagcg
     5161 tggcgccgcc aatcactaga ccaatgccgc ctaagatatt gttacccata agacccacga
     5221 ctcgatctaa cagggtcatg gaaaaaccta aacgtaattt agggttttgc tgcttaagat
     5281 catccgcgtg actgagctct tcggccagtt ctttttcttg gctaagttgc ccgctccggg
     5341 ttaagtgagt accggcaata gcgcggtagc tatcgccacc cagcgtgctg ggtagcccct
     5401 ggttaattaa acctcccgag aaatataaag ccacataccc aagcaagctt ccctgaaagc
     5461 ctgctcgttg cataaataaa ccccagcgta gaccgccaca gataaaggca gcagtaatac
     5521 aaagaccacc cgcaacgagc cactgggctt gtaattgcag ttcggactca aacagggctt
     5581 tccaatcgat accgctagtg gctttccata acaacgcaat cgatagaaca atccgaatgg
     5641 tgggccagct gcgctttagt attactttcc attgagagct catggacgta aggataatgc
     5701 ctcgcgctct ggttttgact ttccacccca gccttgacaa taactaagtt caaattggct
     5761 tagagcctga cctaaatggg ctatccgtaa gcgatctagg gggcgacagg ttctaaattg
     5821 cccttcaccc accggtgcct taaatgacat ctcagaccag ttcagtaaga tgacatttga
     5881 tccctcggga agcttaccaa accacaaatc aaactgatcc tgacgatcat ccaacacaaa
     5941 aataggtgtt ggcttggcgt accatgccac ccgactagcc aatgtccaat tttgaactgc
     6001 aatgccgctc gccttcaatt cattgaccaa gagattggcg cgcgtggacg cagaacgcca
     6061 gccgtataaa tcggccaagg gatttgtctt gaactgactc gcaatcggat agcctgcggt
     6121 cataaccaat gtcagtccgc taatcactag agtaccttgg accgccagaa taagagagat
     6181 caaccagcgt ttaccgcttt gccaccattg cgcaagaccc agccctgcaa tgggggctaa
     6241 acaaaaccac gcaggggtgg tccagtgcgg caagcctcca ccccccgaca aaatagcgaa
     6301 gacactaaat ggcaaaacaa agaacactag caagatactc acaaatgggc gcacagaaaa
     6361 accgatcgcc cgtgaataca accataatcc gaggagaggc aaaaagccaa agaccattac
     6421 ctgtgccccg acgaatgcag caacacgtcg ccatagccaa gtgccaccac tgccatgatc
     6481 gatttgatat ttaaatgaga tccaatcgtg ttgggcattc caaagcaaca cggggctaat
     6541 gagaagaagt gcgattgcaa tggcactata aaaacctgcc tgctttagaa aggtaaagcg
     6601 gggaatgctg atacaggcga tagcgaaggc aagcacaaac agaatggcag tgtacttact
     6661 aagacctgct aaaccaaaca ataaacctaa gataatccat tgataaaaac gtagttgccc
     6721 atcttgttta agccattgca atgccatcca gaggacacca aggcttagcg gtgttaataa
     6781 agaatcagg
//
LOCUS       DDPQ01000123            7181 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_6229,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000123 DDPQ01000000
VERSION     DDPQ01000123.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_6229"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    5918..6042
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2006 a         1771 c         1489 g         1790 t
ORIGIN      
        1 atatcaaaca actcaaaggc ggttccaaag aagactcggt caacttccgt ataggggact
       61 ccaaacttta gattggtacc gactgattta atttgatatt cagaatcgcc tacgtaatac
      121 aggggcttac tcgaacgata gaacaaatcg gtatagcgac taatgccatt ctctgtaaag
      181 tatgggtcgt attgagaaag ggctaaggac tggttaattt tacccagtgc gaaatttaag
      241 ccaatactag tacctgttcc aaaggcattc tcttgattaa tacctgcgga caggattaac
      301 ttctcagtgg atgagaatcc agcaccaatc gagatcgccc ctgtaggctt ctcctctacc
      361 ttcacattaa cgtcaacctg atctggagag cctgggacat cggccgtggt gatttcacta
      421 tccttaacat agcctgtacg gttaatgcgc tcctttgata gtcgaatttt ttcgctatca
      481 aaccaagagc tctcaaactg acgcatctcc cgccgaataa ctaaatcacg tgttttggca
      541 ttccccgtaa tctcaacctt gcgaacataa acacgccgtc cggcatctac caccaaggtg
      601 atatcaacct cattgagatc tcgacggata tcaggctgag gattaatcac tgcaaaagcg
      661 tatccataag agcctaattt ctcagcaatc gctttggtgc tttcggctaa cttggcagat
      721 gaaaaggtct cacccggttt aacggtaaga agagaccgaa actccttctc cttgcccagt
      781 aactcgccag ccaaactaat gttcttaaca gtaaatttat tgccctcacg aatactcaca
      841 gtgagataaa tatctttttt gtctggtgta atcgaaacct gggtcgactc aatcacaaac
      901 tcgagatacc cgcgatttaa ataataggaa cgtattgttt ctagatcagc ggtcagtttt
      961 tgcttggagt acaaattatc ttttgaatac caagataacc atccccccgt ttgtaattgc
     1021 atctcgctac gcaaagtgct ctcactgaaa gccttgttac caataaaatt aatttctttg
     1081 atcttggcag ttgggccttc atcaatattg aaaaagatag caacctgatt gcgcgcccca
     1141 ggcgtaaccg tggttacaac ctcggcagca aaaagcccct tgctcacata ctgacgcttt
     1201 aactcttgct ctgctttatc aatcagggct ttgtcataaa aacgagcatc agcaacacca
     1261 acggtacgca gggacttacg aacaacctct gagtcaaatt ctttcatccc cgtgaactca
     1321 attcgcgaga tggttggacg ttcttccacg atcacaatta aaacatcacc ttgcgcctga
     1381 atctgcacat cccgaaagaa gcctgtggca tataaagcct tgatagaatc tgctgccttc
     1441 tcgctcgtaa agcgctcacc cacttgaaca ggtagataac taaacactgt tccaggctcc
     1501 acgcgctgca aaccctccac ccgaatatct ttaaccacaa atggctctgc agcatgcaca
     1561 gactttactg ggataccaag cattaaaacc agagatgcaa tcacataaat cgatcgctta
     1621 agaattgcta agagagaggt tttagaacgt ggcattagga cggaaaaaat cgttgtaggt
     1681 cattaaatac agcaaaaata gttaaggcaa tgatcaatat aaagccaaat ttttgaaaga
     1741 tctcttgaag ctcaatcgac aagcgttttc cagagaccaa ctcccatgca tcatacagga
     1801 gctgaccgcc atcgagcatg ggtaaaggca ctaagtttaa taagccaata ctgatgctca
     1861 aaagtgccaa aaaggataag aacgactgcc aaccaaactg ggctgacttg ccagccatat
     1921 ccgcaatact aatggggccg ctcaattgct ttagagaggt cgatccagta atgagccctg
     1981 tcatcatgcg ggttgatact ttcgtgatta accatactcg ctctcctgca taaatcaaag
     2041 catcaagggg gtctagttga agctcgaacc aagtggattc tcctttgtca tctttgcccg
     2101 gcatcggtaa gatccctaag cgtgcaaatg ggtctgaatc tggtgtcagc cgaggcaagt
     2161 tctcagccct aaactgaacc acatggctct cgccatcctc tgattccagc tctaatgaaa
     2221 agccctcttc tgcggtaatg gcatccaata atctccaacg caaatcattc caactcacta
     2281 ttggtacaaa ctcactatcg gagtctaact tcatagcaag aatctcatca ccagcctcca
     2341 tatctaattg ggcggccaag gtttgtgcgg caggagcttt aatctgagca ggcagttgct
     2401 gcacaccgtt gatgtaaata aatgccaata ggagaatggc tagcaaaaaa ttagccaacg
     2461 gtccggcagc aacaataaaa gagcgctgcc ataaaggttg agtatcaaat gcacaaggcc
     2521 tatcttcatc actaatgatt tgctcagtat cgcggccgtc gagtaatttg acgtaaccac
     2581 ctaatggaat ggctgcaata acccactctg tttgcgattt cgaccgaaag cttaaaaggg
     2641 gttttccaaa gcccactgca aaacgtaaaa cttttacacc gcacaaacgt gctgccgcgt
     2701 agtggccaaa ctcatgaaaa ctcaccaaga taccgatggt gaccaaaaaa taaagaaggg
     2761 ttgagaggac ttccacgaac taggcctgta tcaaataagt agtggcggct tctcgagcct
     2821 tacgatcaga ctctagtaca tcctcgatcg attgggcctg tttgctctct aggtgattaa
     2881 gtgttgcctc aaccacttgc gcaattcgga gataaggtaa cttgccactc agaaaagctg
     2941 ccactgcgat ttcattagcg gcatttagga ttgttgggct tgtgccgccc tgctttgcag
     3001 ctgcaaaagc gagtgcaagg cagggaaagc gctttagctc aggctcggta aaatgcaaac
     3061 ccgataactg ggttagatta agtggtgcga ctcccgcatg aattcggttg ggccatgcta
     3121 gaccaaatgc aatgggggtt ctcatatcag gctggcctaa ttgagccatc accgaaccat
     3181 cgcggtagcg caccatcgaa tgcaccacgc tctccggatg aatcaatacc tttattttct
     3241 ccaagggaag atcaaataac caatgggcct cgatcacctc taagcctttg ttcatcatag
     3301 tcgctgagtc tactgaaatc tttcgcccca tcacccaatt tggatgtgcg caggcttgat
     3361 ctggggtaat tgaagcgaga tcctctagag gggtgtttct aaacggcccg cctgaggcag
     3421 tcaaccagat ctcctcaacc cccagcagct ctttcatgct aggcgtgtgc tttgcactca
     3481 taaattgagg gggaagacat tgaaaaatag cattgtgctc gctatctatg ggcaataact
     3541 cagcaccacc ctcacgtaca gcattcataa atagatcgcc tgacatgacc aaggcttctt
     3601 tattggcgag taatacgcgc tttcctaatt gtgccgctct taaagtgggc aacaatcctg
     3661 ctgcgccgac aatggctgcc atgaccatat cgcaatcact atctgctact gcggtaatta
     3721 atccctcttg tccatacaag acctgggtag cagagccctt cccttgcaaa agggtagaaa
     3781 gggattgcgc atcttctgca gaactcaata ccgcaatggc cggcttaaac tccaaacact
     3841 gttgcgccaa tcgttcgatt tgttggccag cagttaatgc cacgacctta aattgctctg
     3901 ggtgagcccg caccacatct aatgtgctca tcccgatcga accagtcgat cccaaaatac
     3961 ataaatggcg ggtctgtaga gaattggtca tagcaaggtc aggatagcaa gatttatgct
     4021 cttaccatta tggagaataa agtggccaaa ggcatcacag gcaataaggc atccactcga
     4081 tctaaaaacc caccatggcc tggtaataga tgactactat ctttaacttt tgccaatcgc
     4141 tttaattgcg attcaaagag atctccaaga acactaaaca gaaccaatat cgcggtcata
     4201 aaaagcattg ccccccaacc ccatttggcc tgccaagtac caaaaagggt ctcactggga
     4261 gggaaataca tacaacatag aatcgcatag attaggcaga gtgcaaggcc gcctagcgcc
     4321 ccctctatgg acttaccagg actaatttga ctcgccagtt tgttcttgcc aatggcgcgc
     4381 cccacaaagt aagccccgat atctgcaagc caaactaaaa tcatggcact aagcagccaa
     4441 actaaaccga tttctcttaa gacatcgagc gatagccatg cagcaggcaa aataatgaag
     4501 ccgagaacgc taaatacaag gcgccagcgc aataaggata gatgcaagcc ttgagacagt
     4561 atgaatggca ttccgaatag ccagaaagca agagcaatgc tcaatagcgc gatttcaatc
     4621 gagggttctg cactaagcca cagaagtgct acgatgactg cacaaattag ggcatagatg
     4681 taagcagacc gatttgcatt gggagtaatc aaacgactcc actcccatcc tgccgccacc
     4741 aacatcataa aaaatagtgc agagagataa acaggaggaa gcaggaatag cactggcaat
     4801 aaaattgcca acatgatcaa agctgttatg acccgtgtct taagcatccg aactagactg
     4861 cgtcgcttaa gggcatcacg ttcaattcat ctgcaagctg tgcactggtt cgaccaaaac
     4921 gtctttcacg ttgggcatac caagtaaagg ctttttgcaa ctgagttgca tcaaagtctg
     4981 gccaaagggt atcggtgaaa tataactctg tgtaagcaag ctgccagagc agaaaattac
     5041 tgactcgctg ctcaccaccg gtccgaataa ataaatctgg ttcaggcgca taggccattg
     5101 ataaatgggg caaaaggaga gattctgtta gatcggtagc ttgcaaatta ggatttgcga
     5161 tcaatacctt ttgcattgcc tgcaaaatat cccatcggcc tccgtagtta gcggcaattg
     5221 ttaaggttaa gcgattgcag tctttggttt tttcttcaga gaactcaacc atttcagtaa
     5281 tcgcagttcc aaaacgactc agatcaccaa tcatctttag gcgaatatta ttttcagcta
     5341 aacgagcaac ttctttcttc aaagacttga gaaagagtct tgtcagaaag ccaacctctt
     5401 ctggaggtct acgccagttc tcagagctaa acgcgaatag agtgaggtac tgtacgtttt
     5461 ggcgaacaca ctcttctacc acctttcgta ctgcctctaa accctctgaa tggccagcaa
     5521 ccctcggcat aaaacgcttc gttgcccaac ggccgttccc atccatgatg atggcaacat
     5581 ggcggggaat acttcctacc gcagggaccg cgagcgtaga gctagtattt gcttggctca
     5641 ttagacaggc tcacgacggt caaaccgtca tgatttcctt atccttctca actaccaatt
     5701 tatcaacatc tgcaacaaag cggtcggtca gtttctgaat ctcgtcttgc gcacgacgct
     5761 cttcatcctc agaaatctct ttatctttaa tcaggcgctt gaggtgctca ttggcatcac
     5821 gacgcagatt acgaatagct atcttagtct cttcaccttc agacttaact accttggtca
     5881 actccttgcg acgctcttca gtcaaaggag gcattggnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtcacatt aatagttctg
     6061 gcatcagcca aacctaaatt agcaacctgc gagatggggg taggattacc gtaataatcc
     6121 accatgatgt gttccaagat accgggatta gcgcgccccg ttcttatttt tgcgagatta
     6181 gccttaaacg cctcaatcga cttctgcatc ttttgatctg cattggtctt tatttgtgca
     6241 gcagacatgc cactctcctt tttatacgtg tactagggta ccttctggct caccacgcac
     6301 aacgcttaat aaagcgccgg gcttgagtat tgaaaatacc cggattggta atttacgatc
     6361 acgacacaaa gcaaatgcgg tcgcatccat cacttgtaaa tttttaatga gtgcttcatc
     6421 aaaactgatg gtggaataca aggttgcgtt tgggtcctta ttgggatcgc tactgtagat
     6481 gccatccact tttgtggcct taagcattac ctctacaccc atctctgcag cacgtaatgc
     6541 cgccgcagta tcagtggtga aaaatggatt acctgtgccg gctgcaaaga taacgacctt
     6601 accttctttc atagcgcgga ttgcacgtgg tcgaatgtag ggctctacca cttgatccat
     6661 gcgcagggca gattgcacgc gggcctcgac ccctttttgg cgcaatgcat cttgcaaggc
     6721 tagcgagttc atcatcgtgg ccaacatacc catgtagtcg gcagtggcgc gatccatgcc
     6781 ggccgcgccg cctgccactc cacgaaatat attaccgccg ccaatcacaa tcgcgatctc
     6841 cacaccaagg cttagcactt gagcgatttc agtgaccatt gaatcaattg ttttaggatt
     6901 gattccatag gcatcttcac ccattagggc ttcgcctgat aacttaagaa gaatgcgttt
     6961 gtatccgggc ataaggttta agcagttgcc tttgcagctg caacttgagc agcaacctca
     7021 gcagcaaagt catcttgacg cttctcaata ccctcgccga caacaaataa gctaaacgac
     7081 ttgatggtgg tattggcagc tttgagcatt tgctcaaccg tttgcttatc gttctttaca
     7141 aaagtttgat ttaagagcga tacctcttta aggtattttt g
//
LOCUS       DDPQ01000124            4320 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_9445,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000124 DDPQ01000000
VERSION     DDPQ01000124.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_9445"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT         1130 a          981 c         1052 g         1157 t
ORIGIN      
        1 aatcatgatc cccgcgctag tatttatgat gcttcgcaaa cccgtgtttc ggctgatggt
       61 aagttggtaa aggtcttgtc ctggtatgac aatgagtggg gatactcggt gcagatgctc
      121 aatgcagccg aagccttgat ggcggtataa atcattggct aggaagcctg ccacaagatc
      181 caaaaagcaa gatccagatt ctgctttagt tgaagatatt cgcctgctag ggcgaatcct
      241 gggtgatgtt attcgggagc aggagggggt tgatgttttt aatctgattg agaccatcag
      301 aaccctgtcg gtccgctttc atcggcatag tgacgagcaa gccaataaag cactcaagaa
      361 gcttttaaag gggttgagcg cagatgagtc ggtgaaggtg attcgggcct ttacctactt
      421 tagtcatcta gcaaatctgg ctgaagatcg ccaccagtta cgtcggcaag cagcgcaaga
      481 gcgccttgct actaaacaag agggtagtat tgatcttgct ctcgagcgaa ttcgggcagc
      541 tgggattagc aaccaagcaa tatcaaagac cttagcccat agccatcttt ctcccgtatt
      601 aactgcacat ccaaccgaag tacagcgcaa gagtatcttg gatgcagagc gtagtattgc
      661 ccaattactt caaatccgag atcaaattaa agatcgtgcc aaggcttttc atctcaagaa
      721 agatgtcttg tgtgagcgcg agttatccga taacgagtca ttgatgaagg cgagggtgat
      781 acagctctgg caaacccgct tattgcgggt gaccaagctt aaggttgttg acgaaatcga
      841 gaacgcgctt agttactatg aagctacttt tttgcgagag attccgaagc tctatgccca
      901 actcgaggat cgtttaggaa accagccagt tgcctccttc ttaaagatgg ggcaatggac
      961 cggtggggat cgcgatggaa accccaatgt gactgctgag actttggact atgcactcag
     1021 acgtcaagcg gatatgatct tgcgccatta cttaacggag gtgcattact taggaacgga
     1081 gctatcccta tccgctttat tggttgactt tccaaagtcc atgcaagagt tggcaggacg
     1141 ctcccccgac accaacgaac accgtatgga cgaaccctat cgtcgcgcac tcaccggaat
     1201 ttactcccgc ctcgccgcca cacttaagac tttgacgggt ggagatgcgg cacgccatgc
     1261 agtgacacca caaaatcctt acacaagcgc tcatgagttt ttagaagacc tcaaaatcat
     1321 tgagcattct cttcgttccc attcagcaca ggctcttgtc aatcaacgct tacgaccact
     1381 aattcgctcg gtggaggtat ttggatttca tttagcgacc gttgatttac gccaaagttc
     1441 tgataagcac gaagaggtaa tccatgagct tctattggta gcgaacattg aaaacaatta
     1501 ctcaactctg aatgagttat cgaagcaggc gatcttgctg caattgttaa aggaagctag
     1561 acccttgagg gtgattgggg ctaattactc aagccacacc ttagccgaac taaaaatagt
     1621 tgccttagcc aaagaactca gagagcgctt tggaagtgat gctatacggc actacatcat
     1681 tagtcacacc gagaccgtca gtgatcttct tgaggtcatg ttgctccaaa aagaagtagg
     1741 actcatgtac ggtactttag atacaaaatc aaggattgat ttgattacgg tccctttgtt
     1801 tgaaaccatt ggcgatctgc gtaatgcagc tcccatcatg tctgattttt ataccttacc
     1861 aggcattgca aatttgataa aacgttcggg tggtgagcaa gatatcatgt tgggctactc
     1921 ggatagcaat aaagatggcg gcatctttac tagtaattgg gagttgtatt gtgctgagat
     1981 tgcactcgtt gatctatttg aacgcttagc caatagccat aatatccaac tacgtttatt
     2041 tcatgggcga ggaggtacgg tgggtcgtgg tggcgggcct agctatgaag caattttggc
     2101 acaacctcca ggaacagtgc gtggccaaat ccgtttgacc gaacaagggg aggtgattgg
     2161 atctaaatat tccaatcctg ccattggacg tcaaaacctc gaagcactca ttgccgccac
     2221 gcttgaggca acactcttac agccaaccaa accagcaagc gcaatatttt tagagaccgc
     2281 tgcacaaatc tcgcaagcga gtatgaatgc ataccgatcc ttgatatatg aaactgatgg
     2341 ctttgcagag tttttctttc aggcaacccc tattcgagag atcgcaggac ttaatattgg
     2401 ctcgcggcca gcatcgcgca aggctagtca gcggatcgag gatctgcgtg caattccatg
     2461 gggctttagt tggggacaat gccgcttaac gctacccggt tggtttggtt ttgggtctgc
     2521 agttgattct ttattacatc aatccagtcc tgctaaacaa aaagctgcct tagccctgtt
     2581 acaaaagatg tatcgccagt ggccattctt tcgcaccctg ttatcaaata tggatatggc
     2641 tttatcgaaa gcagatcttg atttagcagc gctctacagt gagctggttg acgatgtgcg
     2701 cttacgcaag aagattttta ctgcgctttg tgctgaatgg gataagacgg tgcatgcgct
     2761 cactcgtatt actggcgaga agcagcgctt agctaataat cctatcttgg ctcgctcgat
     2821 tcgtcaccgc ttcccctata tcgatccctt gcaccatatt caggtggagt tggtgcgacg
     2881 ctatcgcgct ggccataagg atgaacgggt ccagcgcggg attcatttat cgattaatgg
     2941 gattgcttcc ggactgcgta atacgggata gcctccaaac cagtaaaata atgaattaac
     3001 cctttttatc ttactcaata aggaatttgc atgccaggct tgcttcccaa tgtagaccct
     3061 gatggtttac ttgaattctc ggtggtttac accgaccgct ccctcaatca catgtctgct
     3121 gagtttaaga aggttattac tgacatctca gcaatcttga aggatgtcta taaggctcat
     3181 tccacagtgg ttatccctgg gagtggtact tatggcatgg aggcagttgc acgccagttt
     3241 gctcctggta aaaaatgctt aattattcgg aatggttggt ttagttatcg ctggacccaa
     3301 atttttgatc ttgagaaatt tacgaaagac attcacatta ttaaagggcg tcagcaagag
     3361 gtcagcgcac aagggccata tgcacctcct ccgattgaag aagtagttgc atttattaag
     3421 aaagagaaac cagaggttgt ttttgctccc catgtagaga cctccgctgg aatcattctg
     3481 ccccctgagt atctgcgcgc tattggcgat gcagttgagt cagtagacgg cttatttgtg
     3541 ctcgactgta ttgcctccgg tgcaatgtgg gtggatatga aggcgagcaa ggtcgatgtt
     3601 ctgattagtg ctccacaaaa gggttggagt agctcccctt gctgtgccct gattggtatg
     3661 agtgagcgtg cccgccaaaa gatcgaacac actcaaagta atagcttctc gcttgatctc
     3721 aagaagtggt tgcagattat ggagacctat gagaagggcg cgcatgtgta tcacaccact
     3781 atgccaaccg acgcattgaa gatcctacgg aacgccatga aagagaccca gtcgcacggc
     3841 tttgattatt taatgcaaca gcagattctg ttgggttcca aagtaagggc attaatggtc
     3901 tctaagggat ttcctagcgt ggccgctgcc ggttatcagg ctccatgtgt cgtggtgagt
     3961 tacaccactg acccagacat gcaatctggt aaaaagttta tcgatgtcgg cttacaaacg
     4021 gctgcggggg taccattgca ggtgggtgaa agcgctgagt tccgcacatt ccgtctcggt
     4081 ctatttggtc tagagaaact cttgcatatc gagcgagcca ttagtcatct tgaaacggcc
     4141 atgaataaaa ttactgcgaa gtaattacga ttccatttta agaactgcaa acgcagcttt
     4201 caggcgctta gagtaaattg ctttaagcgc ctttactttt tctggggtcc attgccaata
     4261 cccctgcatc gtcttgacac cagatttacc tgctatcacc atatccaaga tggattgagg
//
LOCUS       DDPQ01000125            7571 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_4563,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000125 DDPQ01000000
VERSION     DDPQ01000125.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_4563"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT         2191 a         1605 c         1797 g         1978 t
ORIGIN      
        1 taaacgccca cttgaataat cttacaaaaa taatcgttca ggtgattttg gataagcccc
       61 ttaaagaggc ctttgattac atttgggagg aaacacaagc agggatcaaa ccaagtattg
      121 gaatgattgt ggaggctcca tttggaagag cctcagttgt agggatcgtg attggggtga
      181 gcgctcactc aaatattgag ccatcaaaat taaagtcagt aaaggccttg gcaccactac
      241 cctgtttgga taaaaaaata ctagagctgg ggcaatttgc atcgcattac tatgtttacg
      301 gccttgggga aaccatgatt ccggctattc caaaatggtg gagaagcccc aaaaattggc
      361 agaagggctt gcgcgcttcg ccagccaaga ggacaaatac tcagaccgct ttgcaatcgc
      421 acgatcaggc gatacgtccc gagcaactaa attccgctca ggcggatgca ctctcgcagc
      481 tttgtgcaca caatcgaaaa gagtttcaga ctattttttt gaatggcgtc accggtagtg
      541 ggaagactgc ggtttactta aattatctcc agcatatctt aagtaatgat catgacgcgc
      601 aagtgctggt actactgcca gaaataaacc taaccccaca actagagcga agaattgcaa
      661 gccatatgcc aaatcaagaa atggccgtat tgcatagcgg cttgagtgat aaacaaagag
      721 caatttcatg gcatgcgatc ataagcagaa aagccaaggt cattctcggc acccgcttat
      781 ctatactgac ccctatacca aacttagccg ccattgtggt tgatgaagag cacgatagtt
      841 catttaaaca acaagaaggc ttgggctact ctgccagaga ccttgcaatc tggcgagcaa
      901 aaaatgaaaa cattcctgtt ctactgtctt cagcaacgcc gtccctagaa acatggcacg
      961 ccattgagcg cgggcgctat caagaaatta aattgagcga acgcattcat caggcgcaaa
     1021 tgcccgaagt cgagttggtg cagatacaag aggcggataa aaaccaagcg ataagcacaa
     1081 cccttagaca agccttacaa aaaaactatc aacaaggccg acaagctttg atattggtga
     1141 atcgacgagg ctttgcaccg gttcttactt gcggatcttg tgcatggctt tcagagtgcc
     1201 cagattgctc tggctatatg gtcatgcata aacagcttgg atcaagaaag tcccccgccc
     1261 tatgttgtca tcattgtggt ttgataaagc caattccgct atcgtgtcca aaatgcggtg
     1321 atagcgattt gcatccattg ggtaggggca ctcaaaaaat agaggagcaa ttagaagaga
     1381 ttgttccgca cgccaaagtt ctgagagtgg atgccgatac tgcgcgcacc gggaagttaa
     1441 gcgaagcctt gtttacaaaa attcatcaag gtgaggctga catgattgtt ggaactcaaa
     1501 tgcttagcaa agggcatgat tatcaaaatg tcggattggt atgtgttttg gatgcagacg
     1561 ctagactgta ttccaatgat tatcgtgctc ctgaagcctt gtttgcccaa ctcattcagg
     1621 tggccggtcg cgctggacgt tcgggtgggg ggcataccaa gatgctgatt gaaacccgtt
     1681 atcccaatga cccgatctac caatatcttc gcaattacga tgtcgcagga tatatgaagc
     1741 accttcttaa ggagcgtcaa gagagcggtt tgccacctta tgtttgccat gctttggtgc
     1801 atgcagaatc aaggtacatg agcgattcat tggagttgct tgccgaggca aaaactttaa
     1861 ttcaaaaaaa tagtaacgct aataatggat tgatctgctt tgatcctgtt ccaaaagcat
     1921 tagcaagagt gggcggaaag gagcgcgcac aattattaat cgaagcagat agtcgggctc
     1981 aactacaggt tcaactcaat tgcctagatg attttctgcg caagcaatct aatggccgta
     2041 ttagtaaaaa agggaaggta cgctggtcta ttgagcgcga ccctttgttg atataacaga
     2101 ctaaagggtg gcatcataag aagcggagga aagcaatgac ccaagctctg acgtgagcaa
     2161 agccttatct tccgacttga ttttaaaaag ccataggtca taaggtttgt cattaatggt
     2221 ttcgggcgta tcaattgccg cttgattaat gaccgcaacc gttccgctaa tgggcgcatg
     2281 gatatcgctt gccgctttta aggactcaat gcttgcacat gcctgcccct gcttgatctc
     2341 ggtgttgagg gccggcagtt gtacaaacat tacatcccct aatgcctctt gagcgtgatt
     2401 actaatgcca acccaataca tttggtcatc gcccagcttg acccattcat gcgtgtgagc
     2461 aaacaataga ctgtcgagat tattcataag aatattttat cggtaattaa tcaataatat
     2521 ttgatcaaaa caatcatgat cctgaatcta gcctttgggc ctaatcgccg gttaagcgag
     2581 agagaaaccg tgagtgtggg tgaacacatc ccctaatttt tcattgctgc ggcagcaagt
     2641 cgtgatgtat cgtgtttttt gattaatcaa agcatatagc tttggtcgag aaagacaagc
     2701 caatccccgt gcggggtaag ttgactatgc ttttgccgag cccgtgcgat gtgtacggtt
     2761 atagagacag ctggcgcaaa tccagcgctg cttgcggtta ctggttggaa tccaggtgcc
     2821 gccttccaag cgcttttccc gactacaaga cgagcaaaat ttaaggctag gagatgtgtc
     2881 ttgggtgggg gttggggtgg tagaagtggt cataacggaa tctgataaat ctgctcgaaa
     2941 ccgctatttt acctcgtttc cccattacta gaggggctta ggagaacccg aacagaatta
     3001 gctgttttat tgccattaaa gtctaaccaa gctttttcct tgaaatcgta aagcttgcag
     3061 gtcttagcgg tatcaaaata ccactgccat gagaaaggct gaacaataat tctttctggt
     3121 aaaagggttt caaggcgatt ttgggccgcc ttcaggttat agagcggaac gctcatgtcg
     3181 acatgatggg cggtatgttc catgatgtgg tgcattaatg caccccaata aaacttgaat
     3241 gttagatgaa ccgtagttga tacaaagggc tgagcgcgga gccattcagc cttgttgtca
     3301 taccaagcaa ctgatgggtg agtatgatga acatacacta caaagccaat cattccgttc
     3361 caaaataaat agggcagcac aaatccgcaa agcaatgaaa gtattaccga ttgctctgta
     3421 acaattgctg aggagatgag cgcaccaagc caaataagag cgaaggcgct aaccaaaaga
     3481 ttgtctttaa gaaagatgga ccgatcggct ggcttatatt ttttattggg gaagaacatt
     3541 ttgttccacc aaatttcaac caagtagtag gtaattggtc cccagccgct tcgatagtga
     3601 cgctccaggg cttttcgcca tgctggcaaa gcgtcatact cgtctttagc aagtggcgcc
     3661 cacacaaaat caaagccctt taaattggtt tgtccgtggt gaacaacatt gtgaccaata
     3721 tcccaaaggc tatatggtgt tagcgagggc agaaaggcaa ttcttcccaa aactttattt
     3781 aaattgcgat gcggggtgaa gctttgatgg caagcgtcat ggcccagaat aaaaatacga
     3841 cctgttacaa atccagcaat gatgccaaat actagcttga ccagaatgtt ctcaaaatag
     3901 accgttgcag caatgcacgc aagccacaat actgaatcca gcactaaaag agcaattgct
     3961 tgaagcgttt gcccttctgt catgggggtt agccaggaac gaataacccg acgatgaggc
     4021 aggggcagct cagggctcaa gggcgtagct gtgttggcta atgcttggct agtatggagg
     4081 gcgctattta attccgacac gttttctttc taagttagcc caaataatag tggtttttta
     4141 ggtttttgga gtgacaacgc tttgaatctg gcgcgcccgg caggaatcga acctgcgacc
     4201 cttggcttcg gaggccaata ctctatccac tgagctacgg gcgccaatag tggcgtacaa
     4261 gtctattgta agtgccccca ctcttaatgc actcggtata attcgaattt gtcaaaaaat
     4321 gttctctaga tataacccca acagaccaaa ttaatatgag tgaagagcac ggtagtttaa
     4381 ttaaaaatac aaaacagctg tccatagctg tgattgcaag cttttttatc cctctgatta
     4441 ttatttttct cttaattgtg tatgtcaaca gcgggaaaaa gacagatacc aatggctctg
     4501 agcggtctac gctggctatc atcaaacccg ttggacagtt aaattttaaa gatgccagcg
     4561 cacctcgtga aatgcagact ggcgaggctg tttacaaggc ggtgtgcgcc tcatgccaca
     4621 actcaggagc cgctggggcc cctaaatttg ccgcagcctc aacttggggt gggcgcattg
     4681 gaacgggtta tgacgctttg tatgcctcag ttattaaggg caaaggagca atgccagcgc
     4741 gcgggggagc caatccaagc gatatcagtg attatgaaat cgcacgagcc gtggtttatc
     4801 tcgcaaactc agcgggtgga aagtttccag aaccaaaggc gcccatagaa aaaccagccg
     4861 ccaagtaagc tttagccctt ctccttgcag gaaagggcta tttgataagc ctcatttttg
     4921 tttaggccaa acatagagct tagggttgta gcgatatctt tactactcat atgcgcccct
     4981 agcaggcttg ctaatcggct aggctcagct tgactggaaa ccgcattttt ttgtgaagcg
     5041 ccaccactta gcatcaaaac aaactccccc tttaagttat tggcgttttc aagccattcc
     5101 gcaatttcgc tgggcttgag caggtaaacg ccctcaaatt tcttgcttag ttcgcgccca
     5161 ataaaaagaa tccgctcttc tgggacgagc ttgcttaatt tgagcaatgt tttggagagt
     5221 cggtgagggc tctcgtaaaa catgctgggc aaggagcttt caatcatttg ctcgatcatt
     5281 cgatcaaatt ctttgctctg actgggcaca aaaccaagaa actggaattg gccgtttgat
     5341 tccactaaca aggagcccgc aattgaaagg gcggcactaa gcgcactcgc accaggaatg
     5401 gggatcaccc gaaatcctgc cgcactcacc tgcatgacaa gacgagcccc aggatcagag
     5461 attcccggtg tgccggcatc ggatagatag gcccagcgct gaccattgcg cagatgatct
     5521 attaggacag cggccgcacc catctcatta tgtttatgta gagctaggag tggcttacga
     5581 atatcaaagc gatttaagag ttgcgcgcta tgtcgggtgt cttcacaagc aattccatcg
     5641 acatgattta aaacatgaag cgctctcaga gtaatatcgc ctaaattccc aatgggcgag
     5701 gcaaccatat acagagaaga agttggaagc tcttgctctg agagaaaatg aaggatagtt
     5761 ttgtccatga ataaataaag ccttatagaa ttgattatcc ccgtaaatat taggaataaa
     5821 cagcatctat tttgggaata aatgagatct aatcagagca taataatcat atgaaaatga
     5881 aatcaatcga tagccttgcg agcagggcaa aacaacattt tgtagatagt attgctgtaa
     5941 aacaagatgg cgcggttctg ttgccaaccg aggttgccaa agcaatagca ttaatgagta
     6001 aatgccttca tgaaggcggc aaagtgatgg cctgtggtaa cggcgggtct gcagcagatg
     6061 cccagcactt tgctgctgag ttaatggggc gttttgagcg cgagcgccgc gagcttgccg
     6121 ctcttgcatt aactaccgat acctcgatct tgactgcgat cggtaatgat tacagttacg
     6181 aagatgtgtt tagtaaacaa gttcgtggca ttgggcgcgc agatgatatt ttgatcgcaa
     6241 tctcaacatc tggcaattcg aagaacgtca tcaaagcaat cgaggctgca aaaaaaattg
     6301 gtatacacat cattgctcta actgggaatg gcggaggcaa gatggctgac ctattaaatg
     6361 gccaggatct gcacttgtgt gtcccagctt cacgcaccgc tcggattcaa gagacacact
     6421 tattattgtt gcattgcctt tgcgatgggg ttgatcatgt tttgtttgat gaggaataaa
     6481 tatgttgcga ttgattaaag ttgcatgtct tttattgatc accattcaga tttctgcatg
     6541 cggggtattt attgttgggg gcatggttgg gggcgcaact attttggctg atcgaagaac
     6601 acccgcggtg caagctattg acttaggcat tgagcttgag gcaaacagtc agctctctag
     6661 gaaatttgga gattctgcac atattgtggt cgtatctttt aatcaaaaag ttttattgat
     6721 tggtgaagca aaagatgagt caatcaaaaa tcaagcggct acagatgtga aagcgatgaa
     6781 aaatgttcgc tctttgttta atgaaatcat gattgggccg aacagcacta taggcgcacg
     6841 cgcaagcgac tcttatcttg catcaagcat taaaacccaa ctcgttttta cggcagacat
     6901 tccttcaaat tcaatgaata tttcagtgga gggcggtcga gtttatttga tgggaatatt
     6961 gagccaacaa gaggctgaaa aggctaaaca aattgttagc aaagtaaacg gggtgaaaca
     7021 agtctttgca ttttttgaca ttatttctga gtcagaaaaa cagcgcttag aaaaagaggg
     7081 taaagccaga acctctcagc cagatacgaa cccaagccca aattaagcgt ttttacaaaa
     7141 aagaggccct taagcctgat tcatataagg gtttaccctt gtttattgca gctcgtcttt
     7201 ttggatgtct gaccttaacg acctttcaaa tcagtaaccc attgatttaa ttggagcact
     7261 acaaaaagga ttgctttttt tgtaggaaag tatagaaata atcgtgaatg ggtgttgaca
     7321 tatatctaag ggtatgtcat agtcatggac ttcgctgatt ttccatagat tttcgatggg
     7381 acagccttct ttaaaaatta gtcaaccgat aattgtgggt actgagtaaa ggcatccagt
     7441 ccttcgggac agatgtaaat aaacagtact catagacagt aaaaagattt ggttttatta
     7501 ccaagtcaat ttcttgaatg agtgcgacga accgcaaggt tcacaggaat taaactgaag
     7561 agtttgatcc t
//
LOCUS       DDPQ01000126           23242 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5672,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000126 DDPQ01000000
VERSION     DDPQ01000126.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 23242)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 23242)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23242
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5672"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1149..1179
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2083..2150
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11868..11933
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15160..15176
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21526..21594
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5878 a         5576 c         5152 g         6383 t
ORIGIN      
        1 attcgctcac gcgttacatc aaattgctta ccaacttctt ccagggtatg gtcggtactc
       61 atttcaacac caaaacgcat acggagtact tttgcctcgc ggggtgttaa tgaatccaag
      121 acatctttca ccacatcgcg catggagtca tgcagagcag cgtcggcagg agccagagta
      181 ttggagtctt caataaagtc gcctagatga gaatcctcat catcgccaat tggtgtttcc
      241 atggaaatag gctctttagc aatcttcatg atcttccgaa tcttatcttc aggaatttcc
      301 attttgaggg ctaaggttgc agcatctggc tcatgaccag tttcttgtag tatctggcgg
      361 ctaatgcgat tcatcttatt gatggtttca atcatatgca ctgggatacg aatggttctc
      421 gcctggtctg caatcgatcg agtaatagcc tgacgaatcc accaggtcgc ataggttgag
      481 aacttataac cacggcggta ttcaaattta tcgaccgcct tcatcaaacc aatattgcct
      541 tcttgaatca gatctaagaa ctgcaaaccc cgattggtgt acttcttcgc aatcgatatc
      601 accaaacgca agtttgctac ggtcatttca cgtttagctt ctcgagcacg tttctcaccc
      661 gcaatcattt gcttattcac ttcttttaac tcattgaggg gcaataccac cttggtttgg
      721 atatcaatga gtttttgttg aagctcttga attgctggga cattacgctc taatagtgca
      781 ctgtagggct tagagtcctt aagtagatta tttgtccagc gtaagttcat ggacatcttt
      841 ggaaaggatt gcaaaacttc agaacggggt acgccaatct tgtctaccaa gatactcacg
      901 attccacgct caagcttcca aacctcatct acttgtgagc gcatcgtgtc acagagtttc
      961 tcgacactct tagcagttaa gcggaatcca agtaactcat tgcgaatagc atcttgcgct
     1021 ttcagataac ctggagagtt ataaccatcc cggtcatggg cacgacgcat tttgtcaaac
     1081 tgagtacgaa tgactgcaaa cttttcgagg gagcgttgct ttaactcttc taactgttta
     1141 gcgcttgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ccatcatctc aagctcatcg
     1201 ggctcatcgg gtgccatggg aatatcttcg gcattcggat caactaaacc atccacaaat
     1261 tggtcaatct ccatctcacc gcttgtaatc ttctctacat tgcccaagat ttcagtaatc
     1321 gttacagggc aagctgacag ggccatcacc atgtccttaa ggcctgcctc aatcttttta
     1381 gcgatcacaa tctcaccctc acgagttaag agatcaaccg ttcccatctc gcgcatatac
     1441 atgcggaccg gatcggttgt tcgtccaaac tcagaatcaa cggttgagag cgctgcctct
     1501 gcctcttctt cggcctcttc ttcggaactt gcggtgtgac catgctcatt aagaagcaga
     1561 gtttcggcat cgggtgcctg ttcgtaaacc gtaatgccaa tatcgtttaa caagccaatt
     1621 aaggtctcca atgcatctgc atcggataac tcatcagaca tcacatcatt catttctcca
     1681 tgggtgaggt agcccttgga cttacccatc ttgatcaggg tctttaaacg tgagcggcgg
     1741 agctcttgct gctcttccgt accaagctgt tgagcagcaa actcttttaa gagtgccttt
     1801 tcttttgctt tacgatcgcg cgccttttga cgatcggtgc ggttatcaac acctggttcg
     1861 ccaactacct cgaccttggg tttacgacct cgcgtttttt taatgggctg gccattctca
     1921 tcgagctcaa tcgctgcttc ttttgctttg agcttagcgg cttttgcatc tgccttggct
     1981 tgctcttttg cagctttttc ttgggctttc tttaaggcct tctctgcagc ggcttttttg
     2041 gccaactctt tagcacttag cttaggagcg gcttttacag acnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caggtacttt
     2161 cgcagatttc gttgctggct tagcgcggac tttagtgaca gacttggtga gaggcttact
     2221 cactttcttg gccgcaacct tcgttacttt agccttcaat gccggcttgg ctttttttgc
     2281 taatggcttt gcagaggttt tgccattgct tactggttta ctggtttttt tactagctgg
     2341 cactttggaa actggttttt tcattgtcat ctttgcaatg gtaggtcgta taagttagta
     2401 attacttaca ttaagcttat ttcgtattga aggggcctct ttttgccgtt tctatcaatt
     2461 tgtaccgaaa tagtctcaag ccatgtaggt aagtggctga ttcaaaaagt attttctgga
     2521 agtgcgtatt atactcgatt gagcaaattt gatccgattt gaactacaaa tttacctatt
     2581 gagtgttgat tttgctgggt ttcctagccc ttcgcaagcc tttcccctaa ctcgcgatat
     2641 cgtgcaatat ccactgggct agcctcattt ttagccattt tttgggcaat ttcggtcatt
     2701 tctgccttca gatctgatcg cccgagcttt tggaaaactg catccaaatc agacttagcg
     2761 cccgcaaaat ccaaatccga gcccaaaatt cgttgacgca atatttcata caaatcagcc
     2821 aatgggctat tggctaactg atcttgaaaa acagcaaaat aagaattggg gtttgtttca
     2881 tttaattgca aagaatcgca ttgctgcagg aggtcttgca tcagttctaa tgccttcgca
     2941 gaacgcctct cggcagtgct caatgcaaac tgtctttgta cttgatccat atccttgcca
     3001 agcaccggat actgaatcaa gaggcgcatg atgtgctctg ataaatcggt tggtgccttt
     3061 ggaggctgac ttggctttag gggcaagggc ttaaatcgtg ttgtggcttg ctttgatact
     3121 tgtcccgttt tgacggaaac ccgtccttgg ttttcgaaag aattttgcaa cgggccctgc
     3181 tttggaagcc ctgatccgga tgaagtgttt tggggagcaa gtttaggtaa atcacaaaat
     3241 tgctctagct cactcacgct cgaggctgtg cgatttgcta actcgcgaag caattgtgag
     3301 cgtagggcaa tcggaggcat ggattgcaat aaaggtttag cggtgtgatg cgttttagct
     3361 cttccctcgg ggctggataa atcattccct tcgctggcaa gcttgaaaaa gaatccggat
     3421 aatggcatcg cttgggatac tgcctgttca aaagtagctg ttccatattg ccgaatatag
     3481 ctatcagggt cgtgttcgct gggcaagaat aaaaaccgaa tttctttatc gtctcccaaa
     3541 attggtaaag aagcttctag cgcacgctga gcagctcgct gccctgcact gtcgccatca
     3601 aacgaaaata cgatgcgatc ggtttgacga agcagcgttc gtacatgaaa gggtgtgcaa
     3661 gcagtcccaa gggtggccac tgcattcgca aagcccaact gtgccaatgc cacaacatcc
     3721 atatagcctt cgcagaccaa aacataacct tgatcacgta ttgccttgcg tccctcaaac
     3781 aatccataca gggtgttccc tttggagaac agtggggtct ctggtgaatt tagatacttc
     3841 ggctcgcctt gatccagtat gcgaccgcca aaagcgatta cttgcccttt gggattgcgt
     3901 attggaaaca tgatgcgatc ccgaaaccga tcataccgtt tagcatttcc ttggctatcg
     3961 ttgccctcgc cctgaatcat taatcctgct tcttgcaaga tcttggcagt ttcatcatta
     4021 ctgtaggaat gaaagacagc ttctaggccc tgccaaccat ctggcgcata acccaaggcg
     4081 tagcgcttgg caacttcgcc agtgaggcct ctacctttta gataatcaat cgcgcgctgg
     4141 gatactttta gttgttgagc ataccaatcg cttgccatgt tcatgacctc actcaaagca
     4201 atcgcttgct tttgtttgat gacatcacgc acactccgct cttctctggg caccactagg
     4261 ccggctgaac gagctagatc ctcgattgca tcaacatacg ataggcctga gtattccatc
     4321 atgaacccaa tggctgagcc atgggcagca caaccaaagc agtgataaaa ctgtttggta
     4381 ggcgataccg aaaaagatgg tgacttctct tgatgaaagg ggcacaagcc ttgatagttc
     4441 gcgcccgcct tttttaattt gacatgctta ccaacgacat caacaatatc gacccgattg
     4501 agcaaatcgg agataaagga ttgcggtatg agtgccatgg gatttgtggt tttactttgc
     4561 tagagcagct ttcaccattc ccgagaccat ccccatgtcg gccttacctg ctaactgagc
     4621 ttttagtgcg ccaatcactt tacccatatc ttgggggccg ctggcaccaa gatcagcaat
     4681 tgttttgctc acaatgccag tgatctcctc ggcagataat tgagctggca tatacgcctg
     4741 taatattttg agctcattgc tctcttgatt aaccagatcg gcacgacctg ctttttcaaa
     4801 ctgcgtaata gaatctttgc gctgcttcat cagcttctca ataatggcca caatcccggc
     4861 gtcatccacc acaattcgct catcaacctc acgctgctta attgctgcca ataacaaacg
     4921 gatggttccc aaacgctcag tttctttagc gcgcatggca gacttcatat cttcagtaat
     4981 ttgctcttta aggctcatgg tgataactcc agaaaagata ggctcttgct acattatcca
     5041 agagattgaa aaacaaaaac ccgcttagtg agcgggttcc agaaaaccta aactagcttt
     5101 gggtcctagt tgtgacccaa aacaacttaa tacagtttct tgggaagcat ctggctacga
     5161 atacgcttgt aatggcgctt tgcagcggct gctttcttgc gcttacgctc agctgtaggt
     5221 ttctcataaa actcacgagc gcgaagatcg gtcaaaagac cattcttctc aatggtgcgc
     5281 ttgaagcggc gaagtgcaac ttcaaaaggt tcgttttcgc gaagacggac tgtggtcata
     5341 ctgttttact cgcttttact cttctattgc tagaaaatga tcaaaattta ctaaaacacg
     5401 gattctagca cgataagccc caataatgat agttctaggt attgagacct cctgtgatga
     5461 aactggcctg gccctatacg actcaaggcc ttgggaacag ggtcagcctg cccatttagg
     5521 cattttgggt caagcgcttc actcccaaat tgagatgcac caagattatg gcggggttgt
     5581 gcccgaactg gcctcccgcg accatattcg gcgagtaatt cccctattaa acaagacctt
     5641 aaaagaatct cattgctccc tagaggccat tgatgcgatt gcctataccc aaggaccggg
     5701 attggctgga gccctcctag taggctcagc ctttgccaat gccttagcaa gctcccttaa
     5761 aaaaccgatt attggagtac atcatctgga aggccaccta ctttccccgc tcttggccaa
     5821 agaaggccca ccgcagcctg aatttccttt tgtcgccctt ttggtatctg gcggccatac
     5881 ccaactcatg ttggtcaaag ggattggcca atatgaactc ttgggggaaa ccgtcgatga
     5941 tgctgcgggg gaagcttttg ataagaccgc caaattgctt ggtctggatt atccaggggg
     6001 tgctgcgctt tcaaaactag ccgagggcgg ccaacggggc cgctttgagt taccgcgccc
     6061 gatgctgcat tcagatgact tagatttttc gttctcgggt cttaaaacag ctgttttgaa
     6121 tcaggtcaaa aaagcagaag ccaacaacac cttgaatgaa aattttcgtc aggatattgc
     6181 ctttgcgttc gttgaatctc tagtggaggt tattacgcat aaggcaatga gcgcacttga
     6241 taagacgggt tgtcgtgatt tagtactcgc aggcggtgtg ggggccaatc gccaactacg
     6301 cgcagcacta acagggatgg tcaataaaaa aggctgggct gttcattacc cacctctaga
     6361 actttgcaca gacaacggtg tcatgatcgc atttgcgggg gcacttcgcc tattacaaaa
     6421 agaatttagc ccccctgact tacagggtag ctttgatatc aagccgcgct gggagcttag
     6481 ttcgtaacgt tactaaggga gactaattcg tgcaaacgca ctatggttat ggatcgactc
     6541 aaagttctca gcttcgatca ttaaggacag aatgcgttga tcatttttta atcgacacgc
     6601 cacatcgcgc actagatcct ctacaaactt agggttttca tacgcatgct cagtgaccca
     6661 cttctcatcc gggcgcttca agatagccca caattcactc gatgcttcgc tctctgcaat
     6721 gcgaactaat tcttctacgg taagttgcgc atctgaatct aatttaacca acatggtgat
     6781 gtgagagcgt tgattatgtg caccgtaatc tgagatctcc ttggagcaag gacacaagct
     6841 catgactggc accactgcct gcaaatacaa atctacctca atttgctcat caacaccttg
     6901 tttagcctcc gccaagtaag taacctggta gtccatccag ctttgtactg ctgatacagg
     6961 cgcaattttt ttaacgaagt gggtatagcg catctgaata cgtccctgct tcgcttgcaa
     7021 tagtggaagc atctctttag ccatggtggc aacgcttgcg ctatctattt ctttacggtc
     7081 ttgctctaag agactcacaa aacgagacat atgagtccct tttttatctg ctggtaaggc
     7141 cacatccatc tcaaagctgg caacggtcga aaatgatcca ttggagctac gcatcaaaac
     7201 aggttgttta attcctcgaa taccaacgcg ctcaatcggc aatgcgcgac catcctgatt
     7261 gctttgcaca tcaggcaatg tattggtctt caaaaaagaa gtattgagat cattcatatc
     7321 cgctattttc cctcaaaatg ccaatagggt cttactaggg ctttattttc ctaccggcac
     7381 ttggcttggc ttgagttttg aggctatgga ttctggagcg agatctggga aacgtttgag
     7441 tattgaggct tcgattccag cagagtctaa gccgcattta gccattaata aattgtagtc
     7501 accatgctca ataaatacat cggggagacc caatgttaat aagggttgag aaatccctaa
     7561 gccactcatt gcctctaagc aagcacttcc ggcactgcct tgcacagagg catcttcaac
     7621 acttacaaag taatcgtggt cttttgccag attttgaatg agctcaatat cgattggctt
     7681 aacaaaacgc atattgacaa cagtagcatt gagcttttct gcaacgtcta aggcggggta
     7741 gagtaaggtt ccaaaggcca agatcgccaa gcgctttgtg ctggcagtcg acttacgtcg
     7801 aacctctccg cgtcccaatg gtaaagtgcg caactctttg gatggcggat tcccaatacc
     7861 agcaccacgc ggatagcgga ctgcggtggg atggtcttgg tgataagccg tagttaataa
     7921 atcacgacac tcagcctcat cagccggggt catgatcatc atattaggaa tgcaacgtaa
     7981 gtaggcaata tcgtaagcgc cggcatgggt agcgccatca gcacccacta aaccagcgcg
     8041 atccactgca aagacaatcg gtaaatcctg taaagcgaca tcatggatta attgatcata
     8101 ggcacgctgc aagaatgttg aataaatcgc gaccaccggc ttcatcccct cgcaagccat
     8161 tcccgctgca aaggttacgg agtgctgctc ggctataccg acatcgtagt aacgctccgg
     8221 aaagcgcttc tcaaattcca ctaaaccaga accctcacgc atcgcaggag taatgccaac
     8281 tagttttgga tcggcttgag ccatatcgca taaccaatcc ccaaacactt gtgtaaaggt
     8341 cttcttagaa acaccgctcg atttaatgcc ttcttgcgga ttgaacttac cagggccgtg
     8401 gtaaagaatg ggatcggcct cagccaacat ataaccttga cctttacggg taatgacatg
     8461 caaaaactgt gggccattcc cctctttggc tagacgtcgt acgttttgca tcatcggaac
     8521 taaagtattg agatcatgcc catcgatcgg accaaaataa gtaaatccaa attcttcaaa
     8581 aatggtagag ggggacacaa accccttggc gtgatcctct aagcgttttg caaactcgcg
     8641 taatggaggt gccatcgaag acagaacaga atcaattcct tttttggtcg ctgaataaat
     8701 attgccactc agtaggcgtg cgagatagcg attaagcgca cccactgctg gagaaatcga
     8761 catatcgtta tcattcagaa taacaactaa gggcatggtc tcgtatacac cagcattgtt
     8821 catcgcctca aatgccatcc ctgcgctcat ggcaccatca ccaatcacag caacagcaac
     8881 acggtcttct cccttcgttt gcgctgcgcg cgccatcccc attgccgcag aaatactcgt
     8941 cgatgagtgc gcagtaccaa atgtgtcgta ttcactttcc tcgcgccttg ggaaacccga
     9001 taatccagaa tactggcgca gtgtatccat ccgatcacga cgacctgtca atatcttatg
     9061 ggggtaactc tgatgaccca catcccatac gatccggtcg tgcggtgtat caaatacata
     9121 atgcaaggca atggacaact caactgtccc caaattagag gaaagatgac cgccggtctt
     9181 ggataccgag tcaacaatat attgtcttag ctcatctgct aaagcgggga gctgatcgcg
     9241 atcaagctta cgaagctcgc tagggtcgtt aattgaatga agcatattat ttttttcggt
     9301 ccacaaccaa gtctgcaaga tagcgtaatg catctgcctc aggtccaaaa tgacttaggc
     9361 tgtttagagc tctccctttt agttcttttg ctagttcttg tgcatagtct aaacccttca
     9421 gggtcacata ggtaggcttg tcattggcag cgtccttacc ggctgttttt ccgagggtgg
     9481 cgctatcggc ggttgcatcc aaaatatcat cgaccacttg aaaagccagt ccaagtgcct
     9541 tagcgtagga atgcaggctt tttaaatcct ctttagaaac ccgggctgca atcccgccca
     9601 gttgaacggc acatattaat agggcgccgg ttttcatggc atgcatctgt tctaaagcag
     9661 gcagatccat ttttttacca acgtgttgca aatcgatggc ttgtccacca gccataccct
     9721 tagaacccga ggccccagca agcgcctcaa tcatggctaa gcgcacagca atagtttgag
     9781 cgccatcggc ttgtgaatgg tctgcaagaa tctcaaaggc gcgggtttgt aaagcatcac
     9841 caaccaatag cgcagtcgcc tcatcaaagg ccttatgaac cgttggacgc ccacgtcgca
     9901 aatcatcatc atccatacaa ggcaaatcat catgaaccaa ggaataagca tggatcatct
     9961 caatcgctgc tgcagcgctg tctaccaatt ttgggtttaa gttttctttt gatccaagtt
    10021 cactcgcggc atataccaat aatggccgca ttctttttcc gccacccaat actgcgtaac
    10081 gcatagcgct gtgcaatagt tgagggattg agttctcaga atctaaataa tgtaatagcg
    10141 cgctctcggc gcgttgtgat tgcgagctaa gccatggctc taattgaaaa tgctgcatat
    10201 tcgagatcga ttgagtcaat taggtctcga tgaacttaac ctgttgttca accttggata
    10261 aaacaccctg acaatgtttt agcagagccg ttcccctctg ataagctgac aaagtctcct
    10321 ccaaggagaa tttgccggac tccatctggc tcactaattt ctcgagctct tttaaggcct
    10381 cctcatagcg caaggcaggg tcaaccgcag gaatagtggc agtagagtcg gattcggctg
    10441 ggttcatttt tttgctaggc atatagcaac atattaatac tgaattgatg agggctatta
    10501 ggggtatccc cttgcccttg aatctatctg gtttcagaga aggatttctg cacaaaattc
    10561 cccgatgggt ataattcttt cttcctttcc aatatcgaca tgtcgatggt tggattggtt
    10621 gttccgctta gcttcggctg actttttcgg gggtggggag aatgactaat ctggctaccg
    10681 cgcagtttct tgcgccgtcc aacttacaac tgccggtttc ggcatatttt gatgttgatt
    10741 tatttcaacg tgagatgcga ttgctcttta agcaaggccc gggttatgtg ggtcacgagc
    10801 tcatggttcc cgagaaggga tcctaccgta ccttagctgc cgagaacgaa ggtcgcattt
    10861 tggtgcgcaa ccgtgagggt catcaaggcg ttgaactact ctctaatgta tgccgccatc
    10921 gtcaagcatt gatgcttaat ggcagcggtc aagtagacaa tattgtttgc ccccttcacc
    10981 gttggaccta cgatcttgaa ggccagttgc ttggggcgcc tcattttggc gataagccat
    11041 gcttgcattt aggaaagtca cccttgcaaa attggcaagg ccttctattt gaaggtcctc
    11101 gggatgtgca tgctgatttg ggcaagctgg gtgttgccaa agatctcgat ttttctggct
    11161 acctcttaga ccatgttgaa gtgcatgaat gcagctacaa ctggaagacc tttattgagg
    11221 tttatctcga ggattaccat gtggcgccct tccaccccgg tttaggtaaa tttgtttcct
    11281 gcgaagacct tcattgggaa tttggttcct ggcatagcgt tcaaactgtt ggcatccaca
    11341 aaggcttaag taagcctggg acaccaacct atcgcgcctg gcacgaggca gtattgcgtc
    11401 atcacaaagg cgaaatgcct aaacatgggg caatctggtt aacctactac cccaatgtga
    11461 tggtcgagtg gtaccccggg gttttgtgta tttctacttt gcatcccctt gctccaaata
    11521 aaactcgcaa cattgtggag ttctactatc cagaagagat tgttcttttt gagcgtgagt
    11581 ttgtcaaagc cgagcgcgct gcctatatgg aaacctgtat cgaagacgat gaaattgctg
    11641 agcgtatgga tgcaggacga gctgctttaa tggccagggg tcaaaatgag gttggtcctt
    11701 atcaaagtcc gatggaagat ggtatgcaac attttcatga gtggtatcgt cgagaaataa
    11761 attacccagc ctagatcacc ttaggagtct ttcatgaacc ctatcattac cgctaatcag
    11821 ttagaagaat taatcaacga gggcagcgat gtcctaatat gcgactgnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgccaga
    11941 agaatgggcc aagacccgtc aaggtttagg aattgctaat cacacgcacg tagttgctta
    12001 cgatagtcat gggggcacct ttgcaagtcg tttatggtgg atgttacgct ccattggcca
    12061 cgcttccgtt caggtcttag acggcggtct cgatgcttgg aatggtagtt ttagtagtgt
    12121 agggagagcg ccaaagccgc taagcgcaat gcccgaagcg tatccctacc agggcgttgt
    12181 gctaattgat gagatggaaa aaaatgttac tgctcaggaa cgtaagatgg tgatcgatgc
    12241 acgagcaccc gatcgctttc ggggtgagaa tgaaactcta gaccccgtag gtggccacat
    12301 accaggggca accaatcgat gctttaaaga aaacttagtg gctggtaagt tcaaatctcc
    12361 ggagctgctg tataaagaat ttgtcttact aatcggccaa caaaagcctg gcacgattat
    12421 tcattcctgt ggctcaggtg tgagtgcttg tcataatctt ttagcaatgg aagcggcagg
    12481 actcagtggc tcacgactac atgtgggaag ttggagcgaa tggtgctcgg atcccaagcg
    12541 ccccattgaa acctaggcct ctacaaaaag agttaatggt ggtgatcggt tagaaacacc
    12601 acaataccga cacccattgc aatcaaaacg atttgacgaa gagactcttg ccagtggggt
    12661 cggcgatgca tttggggaat caaatcactc acggcgatat agataaaact acttgaagca
    12721 ataaccagca aatagggcat tgcttgattg gctttttcca gaaagtaata agacaatacc
    12781 ccaccgatta ctgccattaa gccactcaat gcgttatagc aaagagcgcg cagtcttgag
    12841 aaccctgcat ttaagagaat aatgaaatca ccaatctctt gcgggatctc gtgcgcaatg
    12901 attgcaacgg cagtaaagat gcctacttgt atatccacca taaaagctgc ggctatcaac
    12961 acaccgtcga caaaattatg gatgctgtca cctaccagaa tcatccaacc actgttaccc
    13021 gcctcctcag catcgtgacc atgatgatgg ccgtggccat cgccttcgtg atgatggcta
    13081 tgacgcaata gtgcaatctt ttctaacaag aaaaatccca acaagccacc aagcaaggta
    13141 gcaaacaaat aatggacttg ggcaccagct aactcaaaag cctctggcag agaatgcaag
    13201 aaagtcgttg ccagcaaaat accgactgac agactcacca tgttgttcac aattttggca
    13261 agcagggtta aagacaaact agcggccgtc aggatgctaa tcagtcccgc ccctgcggtt
    13321 accaaaataa ttaactgtag tgtgctgaag gtcacagaga atccaaaaga gattgattcg
    13381 ggccttatgc aacaccgtgt tgcttaaacc aggcgaggca ttttgcccag ccatcttttg
    13441 cagggccttc acgataggtc gcgcgatagt ctgcatggaa tgcatgaggt gcctctggat
    13501 agatctcaaa cttggatgct ttagcagaag gattagagct tgctttagcc aaggcggctt
    13561 tcatttgatc cacggtatcc acaggaatgc cagtatcggc tgatccatac aaaccaagaa
    13621 caggcgcttt tagctcagca gctatctcca caggctgctt aggattattg gcagtcttgt
    13681 cgcccactaa gcgaccatac caagcgacac ctgctttgac ctcaggcatc gtggcggata
    13741 accacgtaat acgaccgccc caacagaatc cagttacggc gactttctta ggatcgccac
    13801 cgttctttac ggcccaagtg attgctgctt ttagatcacc taatacttgg gtgtcaggag
    13861 ttttagcaac aatattgctc tgaatctctg cgatggttcc aaaggtattc ggatcgcctg
    13921 ctcgaataaa gaactcggga gcaatagcta aatagccaag ctttgcaaaa cgacgcgtca
    13981 cgtctgcaat gtactcgtgc actccaaaga tctcactcac tacgatcaca accggtaagg
    14041 ggccggtggc tttttcagga cgagagatgt atgcaggtaa attaaaactg cccacaggaa
    14101 tatcttgctc ccccgcttta atcccagcaa agtcggtctt aattgcttgc gccatcaccg
    14161 gctctgaagc agcaacaaaa cctaggccta aggcgctagc tgtcactgta gctgtggagg
    14221 ccctaaggga tgttttcata aaagcacggc ggctggattg ctcttgtttt atcttgatgg
    14281 tcatcatttg tctcctcaat cattgatttg ttgatacggt taatgcgctt cttcccaatt
    14341 gtcgcccaca ccaatactca ctataagtgg caccttgagt tgcgcaatcg aacccattaa
    14401 ccctggtagg gatgatttta gatgctccag ctcatcgggc gcagcctcaa atactagttc
    14461 atcatgaacc tgtaaaagca tacgggtctt taatttctca cgttccaacc aatcttgtac
    14521 cgctaccatt gctaatttaa tcaagtctgc ggccgtaccc tgcataggtg cattaatagc
    14581 agctcgctcc gcaccttgac gtcgcgggcc gcttgcttta atttcgggta accacaggcg
    14641 acgtccaaag acggtttcta cataacctct ggagcgcgct tcctcgcgag tccgctccat
    14701 gtaagcagca accccagggt aacgctcaaa ataggtagca atgtattttt gagcggcagc
    14761 ccgctcgatc ttcaaattac ccgccaatcc ataagcactc atgccataga tcaagccaaa
    14821 attaatgact ttggcataac gtcgctgttc cgaactcacc tgctctagag gaatagcaaa
    14881 aatctcggca gcagttgcct gatgaatatc tttacccgcc ctaaatgcag caagcagatt
    14941 gtcgtcctcg gcgatgtgcg ccatgatgcg taactcaatc tgcgagtagt cggcagatac
    15001 taagcggtag ccctttgcgg gtatgaaggc ttcccgaatt ctgcggccct cttcggttcg
    15061 tactggaata ttctgcaaat taggttcatt ggatgccaaa cgacctgtca ccgctaccgc
    15121 ttgtgcgtag ttagtatgca cacgtccggt ctttggattn nnnnnnnnnn nnnnnncggt
    15181 aatctaagat tcgagctggt agggggtagt cctcggctaa cttttgcaat acctcttcat
    15241 cagtagatgg tgcacccgac ggcgtcttct tgacgactgg tagttgtaat tgaccaaata
    15301 agatttcagc aatttgcttg ggtgactgaa tattaaaagg ctgttttgca agttcatgaa
    15361 tttctttttc aagggctagt aatttttttc ctacctcttg accttgctgt gctaattttg
    15421 cactatcaat cgtaatgccg ttgcgctcta tgatccccaa gacacgcatc gcaggcatct
    15481 caatatgctc atacacataa cgcaagccgg gctcttgttc taactggggc catagatact
    15541 catggagtcg taaggtgata tccgcatcct ctgcagcata gtctgtcgct gttttcaggt
    15601 cgacctgatc aaacccaatc tgatgagccc ccttaccgca gacatcttcg taacgaatcg
    15661 tcttgattcc taaatgccgc tctgcgagac tatccatacc gtggcttaag tgcgactcca
    15721 atacataaga ctctaacaag gtgtcatgag ccacgccttg catcgtaatt tgatagttct
    15781 caaatacatg gatatcgtat ttaagattct gacccacttt tttagcgttt gggttttcta
    15841 accaaggctt tagttgtgca agaacccagg tgcgatccag ctggtcttca ccagtacgat
    15901 gcgctactgg gatataacaa gcctcaccca ctttgaccga tagtgaaatg ccaactaatt
    15961 cggcttgcat cgcatcaagg ctagtggttt cggtatcaat acatgtaaga gctgcatcct
    16021 gtattttgtg cacccatgtc tgaaaagtag tcttatcggt gatgcataca taatttcgcg
    16081 gtagatcaga cagatgggat gaggatggct tattttgggt agctggacta gcttgaatat
    16141 ctcccttgtc ttgcgaaaga gacttaggca cctcatcgct accttgggta gcaaaatttg
    16201 gtttgctcga gttaccactt tcaccaattt cttttaacca ggtcctaaaa gagtatttct
    16261 caaaaagttc tttgagacga ccatgatctt ctggttttgc atgcaagtca tgtaatgaag
    16321 cgatatgtgg gcttagatca caatctaatt taacggttag aagctcgcgg gctttcggca
    16381 gccagtccaa gctagcgcgc agattatcac ccaccacccc cttgatctct gcggcatgcg
    16441 caattaagtt atcaagatcg ccatactcgg ctaaccattt attggctgtt ttggggccgg
    16501 ctttatgaac gcctgggacg ttatccacag tatcaccaac gatcgttagg taatcaacga
    16561 tccgatcagg agctacccca aattttgcct cgacaccttg agtatctaat ttctcattac
    16621 tcatggtgtt tactaagctg accttatcgt taaccagctg agcaaggtct ttatctccgg
    16681 tagaaatgac cgttgaccaa cccatttcag cagcctgcct cgctaaggtc ccaatcacat
    16741 catcggcttc tacaccagag atcatcaata caggccaacc caaagccttt acaacttcat
    16801 gaatcggctc aatttgggca accaaatcct ccggcatggg cgagcgatgc gctttgtatt
    16861 ccggatagag atcttcccga aaggttttcc ctttggcatc aaacacacag gcaatatgat
    16921 cggccccgat ttcagctcgg gcccgacgca tcatattgac cattccatag atcgccccag
    16981 ttggctcccc agccgggttt cgcaaatcag gcatggcgtg gaaggctcga tatagatagc
    17041 tagagccgtc taccagcaat aattgatgtg ttgacatccc tcaatcgtac aatctagttg
    17101 aaagtgcctg aagaaagcct ctatttaggg gattcccgag ctaaaccttc aggtcatttt
    17161 ttctcaggat aatagagaat attaattatt ccattaaggg cacctatggc tttgaccccc
    17221 tccacctttc ctgcctttgc cctcagcagc cttttggcgc ttggtggctt gcattggagt
    17281 cttggtatag caaacgcaca atcaggcggc actcaagctt tgactaaaga agatctcaac
    17341 cagattaata aacagcccct agtaccccct gcttcgaatc cagcgggatt gggtaaaaaa
    17401 gaggagcgca agcctagttt tgaatatacc gaaacaaccg gcactcagat acgggaatac
    17461 cgcgagagta atttgccgac agaaattgag gttaaatcta cttttggtac ttatgagatg
    17521 tctcctccgc aaactgtttt ccctacgact ggttcgcaac aagacaataa tttactaagc
    17581 gtacccagta ttcgtattcc cttaaagtag atttgaaata gaacggttat ggcggtcttt
    17641 accccagtca gtcttgagga aatcactcct cgcattgcag ctgattttgt aatcggcgag
    17701 gctattcttc ttaaaggaat tcatgggggg atcgagaact ccaatttctt tctaaactgt
    17761 caagacgatg ggcaagttaa tgaatatgtt ctaacaatct ttgagagact ctcgtttaca
    17821 cagcttccct attacttaga gctgatgttg catttggcta atcaaggcat ccccgttccc
    17881 aagccgattg taaatcggca gggtgctcta gtgattgaac tcaaaggcaa gcctgcttgt
    17941 attgtgacca aacttccggg tcaatcagag cttgcgccac agccagagca ctgtgaagaa
    18001 gtgggcagca tgttagctaa gatgcatcta gctggccaat ctttttcttt atcacagcct
    18061 aatttgcgca gtctgtcatg gtggcaagaa accgttcccc tcattctgcc gcacattact
    18121 gactctcaaa aagaactctt ggtctcagaa atgcagtacc aagaaagttt ttttaaatcc
    18181 ggcacgtatc aatcccttcc cgcaggacct tgtcattgcg acttattccg tgacaatgtg
    18241 ctctttacta gcaatcaagg tcaaagcatg ctggggggct tctttgattt ctattttgcc
    18301 ggcaatgata aatggctctt tgatctggca gttactgcca atgattggtg tttaaagcag
    18361 cccattcaaa atggtaccgg ggagatcgat cgagatcgct tacacgctct tttaagtgga
    18421 tatttacagg ttcgtccttt aaccgatgcc gaagaagatg cttggcccgc aatgcttcgg
    18481 gctgcagcct tacgcttctg gatatcccgt ctttgggatt tttacttgcc ccgcaatgca
    18541 caaatgctga ccccccatga ccctagtcac tttgagcgta tattgcttag tcgccgtcat
    18601 acaacttaag gtaagctaag cacccatgaa cctgaactct gtttcgacca atgaaggcta
    18661 tgtctgggtc cgccaaggag tgtggctgtt taagcaaaat ccgcttggct ttctgatgct
    18721 ggtctttatg tatgtgttca ttgcccaact ggctgtgatg attcccttgg cgggcgtttt
    18781 tgctgttctc attttgaccc ctgctctctc ggtaggcttt atgaccgcct gtcgccaaac
    18841 cattcagaaa gaacggattt tgccaacggc ttatttagca gcttttaggt ctaataatcc
    18901 tgaggtaaag aagcggattt tgcaattagg cttggtctat gccgtaatga tttttgcatt
    18961 gagcctcatt gctagcatgg tgattgattt tcaggcatta ctgccatttg ttactaatga
    19021 caaacccatt acccctgaag tcatgcaaac cctgtactac tctctcatga tcggtggagt
    19081 catttatatc ccgattgcaa tgttaatgtg gtttgctcct gtcttagtgg cttgggcaga
    19141 tatgccacct agtaaagcaa tgtttgcgag ttggatggcc tgctggacca ataaaggcgc
    19201 cttcttttat tacatcacta tttggagtgt ggctctcatt gcaatcccat taattcttgg
    19261 gatgctgatg gacgtgcttg gattaagtca gtacgcatcc tttgtcgttg cccctctctc
    19321 aatggcaggg ctcacggtca tgtattgctc cttctttgcc acctggaagg gatgctttac
    19381 tgggaatgat gtagaacagt tgtcgaatca ctcggaccaa ttctaagttt ctacacctta
    19441 atcaatcatt tttttagaag attgacatgt ggtttcttta acttataata cgttgtatac
    19501 attgtattga aggggaaagt agcatgagta cagccgtgtt aactttaaga attaacaata
    19561 aaactaaatt aatgcttgac cgattagcta atgctactca ccgcactaaa tcgtttttgg
    19621 ctgcagaagc tattgagcgt tatatcgaac ttgaggcttg gcaagttaaa gaaatcaaag
    19681 cagctcttaa agaggctgac aaaaaagatt ttgtttctga taaagacttt gcaaaactag
    19741 tgagaaagca tgcaggttaa gtggttaagg actgcagctc aaaatttgga tgatgaaact
    19801 aattatctag ctaaagataa tcctaaggtt gccaaagctt tttttgttca cattattaaa
    19861 aatgttaacc aattaaaaat atttcccgat cttggtcgac ctggtcgagt aagtggcact
    19921 agggaattgg tcatactcaa ctacccttac attattcctt ataggattaa aggagatacc
    19981 gtagaggtgc taagagtttt ccatacttca agaatgtggc caaaattgct ttgatatgan
    20041 nctcactcga ccttcaccaa atttagtatg aaagacctct tgcccaaccc gaaaggtatg
    20101 gccccgtttt ttgttctgct ctagttgctt cgcgctggtc acaatggcat tggtgctttg
    20161 attggtgtca tcacgtacag gcacatcgtc accactccac catgcttgat ccacactatc
    20221 ttgatcagac ttcttcagtg ttgtcccttt ttgccatgag cctccacccc agcgagcatc
    20281 ttttgcttta ggggtgaggt atttgacatt ctccgcgggt aattcatcaa ggaatcgtga
    20341 tggcatgttg tatcgcactt gaccgtgcaa cattctcgac tgagtatgag atagataaag
    20401 cctctctttg gctcgggtaa tcgctacata catcagtctg cgctcttctt ctaggccgtg
    20461 cggctcagaa atactatttt catgcggaaa tagtccttct tctaggcccg tcataaatac
    20521 ggcggagaac tctaagccct tggcggaatg taccgtcatt aattgaatcg catcctgccc
    20581 agcctctgct tgattatccc cagcctccaa ggaagcgtgc gctagaaatc cagccaatgg
    20641 tgagatatct tcaaccccag cttgatcttc tcccggtggc gtcgccgctt gcacgtcttg
    20701 tccataaccc tcttctgcaa caaaggctgt agctgcatta atcaactctt gtaagttctc
    20761 aacgcgatct tgtccctcgc gctcacttaa ataatgctga atcaagccgc tatgctgaat
    20821 cacatactcg acagtctctg gcaaggtgtt gtgcttagtt gcatcgcgca ggtgctcaat
    20881 taagcgcaca aaacccgcaa tgctagaacc ggccttgccc tcaataaagg aggctgccgc
    20941 atagaacgag cactggtgta gcttagcagc atcttgtaat gcttctaaag aacgagcacc
    21001 gataccgcga gttgggaaat taacgactcg ggcaaatgag gtgtcgtcat tcgggttctc
    21061 taaaaggcgc atataggcga gagcatgctt aatctcagcg cgctcaaaga agcgtaagcc
    21121 gccatatacc cggtacggaa tattggatga aaagagtcca tgctcaataa tccgtgactg
    21181 agcgttacta cgatacaaaa tagcaacttc gctacgactc aaccctgcgc gaattaatgc
    21241 cttaatctca tcaatcaacc aagcactctc catgccatcg cttgccgcct cataaattct
    21301 gacaggctca ccatagccag cttcggtacg taggtttttt ccaaggcgct ctacattgtg
    21361 tgcaattaaa tgattggcag catctagaat gtgtccgtgc gagcgatagt tctgttccag
    21421 ttttacagtt actggtttgt attgcttttc ataaaggcgc atattctcca catcagcacc
    21481 gcggaacgca taaatacttt ggtcatcatc gcctaccgca aaaagnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagggca
    21601 ttggtatcct gaaactcatc gatcaaaata tgttggaagc gctcttgata atgcgtccga
    21661 ataatctcgt tgtgtttaag tagttcataa cttcgtaaca agagctcaga gaaatcacag
    21721 acgccttcgc gttggcattg agcatcatag gcttcatata actgaatcat cttcgcctca
    21781 aaatcatcgc ctggatctaa atccttggcg cgcttacccc gctccttgga attggatatg
    21841 aagtactgta attgacgagg cgggaacttc tcatcatcaa tattgagact cttcaataat
    21901 cgcttgatcg ccgagagctg gtcttgggta tctagaatct gaaatgtcga tggcagccca
    21961 gcatctcggt gatgggctcg taataatcga ttacagaggc catggaaggt gccaacccac
    22021 atgccccgcg tattaatcgg taacatggca cttaatcggg ttagcatctc ttttgcagct
    22081 ttatttgtaa atgtaactgc caagatacca attggtgata tttgtcccgt ttgaatgagc
    22141 cacgcaattc gggtggtcag aactcgagtt ttaccactac ctgcccccgc cagaatcagt
    22201 gcggattgag cgtggccatt ggtatcaacc ggtgggaggg ttactgcctc tcgttgttcg
    22261 ggattgaggt tctcaagtaa atctgcatgc atcgtcccaa ttataatttg cctcttatgt
    22321 caaacgctca aaatcctcca caaaaactcg atgctgatag caatccagac ttagcaaaac
    22381 tagcaaagtc ctatgagcct gccccaattg aagctttttg gggtcccgag tgggaaaaac
    22441 gcggaattgc agaggcaagc ttggaggatg gcaaggaaga tttctgtgta cagctacctc
    22501 cacccaatgt tactggcacc ttacatatgg ggcatgcatt taatcaaacc atcatggatg
    22561 gtctggtacg ccatgctcgt atgtctaata agaatacgct ttgggtaccc ggtaccgatc
    22621 atgctggtat cgctacacag attgtggttg agcgacaact cgatgcccaa aaaatatcgc
    22681 gtcatgattt aggtcgagag tcatttctta aaaaagtatg ggaatggaaa gaagaatcag
    22741 gttccaccat cacacgtcag attcggcgct tgggagcttc gattgattgg tcccgtgaat
    22801 acttcacgat ggacccaaag atgtccgctg cagttgtaga ggtatttgta acactctatg
    22861 agcaaggcct gatctatcgg ggtaagcgat tggtcaattg ggatcctgtt cttggtactg
    22921 ctgtatctga tttggaagtt gttagttccg aagaacaagg cagtttgtgg catatccgct
    22981 atccactgtc cgatggtagc ggtcaattaa ttgttgcgac tactcgtccc gagaccttgc
    23041 tgggtgacgt ggccgtcatg gtgcatcctg atgatgagcg ctatcgtcat ctcattggta
    23101 aaactgttga cctaccttta tgcgagcgca gtattccgat tattgccgac tcctatgtag
    23161 acatgagttt tggaacgggt gtggtgaagg ttaccccagc ccatgatttt aatgactatg
    23221 cagtgggtca acgtcatcaa tt
//
LOCUS       DDPQ01000127            3606 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_29465,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000127 DDPQ01000000
VERSION     DDPQ01000127.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_29465"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    682..697
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2069..2167
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          922 a          793 c          805 g          971 t
ORIGIN      
        1 tttggacgag actggcgcta ccccattact tctggagcaa agctctaggc cttcaaacct
       61 tggggatata tccccctccc cattccttgg gtatgattgg gtcataagga gacttttatg
      121 aaactcatta catccattat caaacccttt aagttagatg aagtgcgtga ggccttggct
      181 gaaattggcg tgactggctt aacggtcact gaagtgaaag gctttggacg ccaaaagggt
      241 catactgaac tttatcgcgg tgctgaatac gtagtcgact tcttgcccaa agtgaaagta
      301 gaggttgtgg ttccagatga tcgcctagac tccgttacgg aagcgattgt taaagcagcc
      361 cgcacaggca agattgggga tggcaagatt tttgtgagtc ctatcgaaca tgcaatccga
      421 attcggacgg gtgaacagga tgatgcggca gtctagattg ttctagaaag gaacttcttc
      481 caaagtaatg cttttcgcat tttgatcctc aacccgaact ttgtatcctt gaaaaaagat
      541 atctgcttgg gcaagccctg aggtcatcag cacaaaaggc gctttaccaa aaaatgagtg
      601 cgagccgttt acatccagag actttttctc aagttttcca gtcgcgtctt tgacgcacac
      661 cacctgcttg atcttcgttg annnnnnnnn nnnnnnncag ttttcagaac ttgtgctggg
      721 agccacagca gctactgctg gaacaatggc aacaggagta ggcgacgggc tactcactga
      781 ggtcgggcta agcggagcat cctctttctt tgccgaacta ttggcggtat tttcaatcga
      841 ttctgttggt aacttggcaa cgatcggttc agcattctta tcttttgctg gtagctcaat
      901 attcaaaacg atagccacca caattgcagc aagcgcaaag aaagcatact tagtctttga
      961 tggcttcact tctttttctt cagcgggagg gtcaaagcgt tcaagacgct ctggcgtttt
     1021 ttctaccaca gggggaactg gtggagctgc cggaatagat tgaggctctg gtaaatcagt
     1081 ctgctctgat tgaataggct cttcagacga ttggatatcc tgaactaagt caggaccaaa
     1141 atcaaatacc tcttcgaatg gaagtcctaa atattcagcg actcgtttgg tcgcggaaac
     1201 cttaatcgct aatgaataaa aatggcttcg ttcaccacac tcgatttgct caatttgctt
     1261 tacagaaaaa caaaggtctt tcgacatttt aggaattgag attcccttag cttcgcgagc
     1321 ctttttaata acatccgcac gaatatcagg tagtttgtga tcgttagcca ctatgtctct
     1381 ttttgcttaa atatcagata tttagatact ttataaatta ttccaaatat aacctaaatt
     1441 tattagccta actatgttct ttagggctaa tttagctcta gatggtatcg aacgaggtcc
     1501 tgaagtgaac tggccctcat tttttccatt acgcgcgctt tatggacctt aatggtcgca
     1561 tcggttgtgc ccagtctctc tgctatttct ttattcatga gcccctccac tagcaaggca
     1621 caaacctctt tttctctggg ggtcaaggta ttaaagttct tcttgatctc gctaactttt
     1681 aatattcgct taaattgctt ttggtcatat tccaaggcat ctcggattgc atttaatagc
     1741 tcctcgagat taaacggttt aaataagaaa tctaaggccc ctttctttag tccttttacg
     1801 atctcctgag gatggctttg accacttaaa aagataattg gagttgtaaa tcctattttt
     1861 tctaagcgct ccatcaagtc caaaccggtg atatcaggca tttgcatatc gagcaaaata
     1921 actgcggggc agaccaattt attactggtt aaaaatgagg atgcactctg atagtcatag
     1981 accaaataac ctacgtcgcg caacattcga gataaggaac tgcgcattga atcgtcgtcg
     2041 tcaattaaat agactgtgcc aattaaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnatc tcttaaaatg gcatctttaa tagaaaaccc agaaattacc catgaaaacc
     2221 tatttatgca ttgtttgtgg ctttatttac gacgaagcta gcggccgccc tgaggacggc
     2281 atcgctcctg gaacaagctg ggatcaagtc ccatcagact gggcctgccc agactgtgga
     2341 gcaggcaagg atagctttga aatgatggca atttaggcat ttcttacttc cccccttcac
     2401 agcaagccca gaatggtttc atctggcaaa ataagccctt cccaagccct ttgacctcta
     2461 ttggagagca cattacatgt caaacagcaa ctcatcctca cctaagcgtc taaacgagcg
     2521 ctcccgtatg gttacagaag gggtggcaag agcacctaat cgctcgatgt attacgccat
     2581 gggctacaaa gaagaggatt ttgtgaagcc catggtgggc gttgccaatg gccactccac
     2641 gattacgcct tgcaatagtg gtttacagaa attagccgat gcagcaattg ctgcactgga
     2701 atcctcaggg gctaaggccc aagtttttgg caccccaacg gtatcggatg gtattggcat
     2761 gggtaccgaa ggcatgaagt actcccttgt ctcacgcgaa gtgattgctg acagtattga
     2821 ggtatgcgtt aatgggttat ggcaagacgg tgtggtggtg attggtggtt gcgataaaaa
     2881 tatgcctggt ggcatgatcg ctctagcacg aaccaatgtt ccgggtatct atgtctatgg
     2941 tggcacgatt aaagcgggtc actacaaggg caaagatctc aatattgtgt ctgcctttga
     3001 agccgttggc gaatttacgt ccggacgatt gaccgaagaa gacctcaaag gggtcgagca
     3061 gaacgcctgc ccaagtagcg gctcctgcgg tggtatgtat accgccaaca ccatgagctc
     3121 gtcctttgag gcattaggta tgagcctacc ctactcctcc accatgtcca atgtggatca
     3181 agagaaagtc gatagtgctg ccgaatcggc acgtgttttg gttgaggcga tcaagaaaaa
     3241 tattcgtccg cgcgacatca tcaccaagaa gtccttagaa aatgctgtga gcgtcatcat
     3301 ggctgttggt ggctctacca atgcggtctt gcacttttta gccattgcca gtgccgctga
     3361 aattgattgg accatcgatg actttgagcg gattcgtaag cgcgttccgg tcattgctga
     3421 tatgaaaccc tcgggcactt atttggcacc cgatctccat caagctggcg gcatccctca
     3481 gatcatgaag atcttgttag atggtggact gcttcatggc gactgcatca cgatcagcgg
     3541 gcaaaccatt gcggaagttt tgaaagatat tccctcaaaa ccacgagccg atcaaaaagt
     3601 aatacg
//
LOCUS       DDPQ01000128            7190 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11680,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000128 DDPQ01000000
VERSION     DDPQ01000128.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11680"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1242..1260
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1931..1945
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2899..2951
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3134..3232
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3607..3658
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4376..4457
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6789..6951
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1891 a         1393 c         1615 g         1806 t
ORIGIN      
        1 gtgatctctg catcgcataa tctctatcaa gacaatggta ttaagttttt cactgccaag
       61 ggcgataagt tctcagatgc ctttgagctc gagattgagc aagagcttga gaacgcgatg
      121 ggctgtgtca gctccgataa actgggcaaa gcctttcgtc ttgatgatgc agcaggacgt
      181 tacattgagt tttgtaaatc cacattccca gccgatctca atctcaaagg cctaaagatt
      241 gtgcttgact gtgctaatgg tgctgcttat cacacggccc ctccggtttt tcatgaacta
      301 ggagcggagg tgattgcgat cggcgtatca ccaaatggtc gcaatattaa tgatcactgt
      361 ggtgcaactg caccccaagc actgatcgca aaggtacaag aagtcagtgc tgatattggt
      421 attgcccttg atggtgatgc cgatcgattg cagatggtgg atagtagcgg ccgactcttt
      481 aacggggatg agttgttgta tgtgttagct aaagatcgta ttgatcgggg gcagaagatt
      541 ggtggggtag ttggcacact catgaccaat ttagcaatcg agaatgccat taagagcctc
      601 ggtcttggct ttgaacgggc caaagtaggc gatcgctatg ttctagagtt attacagaac
      661 aagcaatgga tcattggcgg agagggatct ggtcatctac tgtgcttaga tcagcacagt
      721 acgggagatg gcaccatcgc tgctttacaa gtgctcgcag cgatgcaaga aaaacagaag
      781 tccttaaacg atttattgaa tggtgttcag atctttccgc aagtgctgat caacgtcaaa
      841 accaagcctg attacaactg gcagaatgac cttaatctca acactgcaat cactaaggca
      901 gaacaaagcc tggttggtaa gggtagggta ttaattcggg catcggggac ggagccggtg
      961 ctacgagtga tggtagaggc caacgaggca aacctagcgc agcaaactgc tgaggcgatt
     1021 gcagggcaag tcaatacata ttaattgccc cagtccgtat cgatctcatt ttgctttggg
     1081 tcattatcgc gcctattttt acgattaact aagtattgcc agattaggcg gtctttctca
     1141 tgattatcaa tatcatagtg ttggaaccgc tcctctaatt cctttatttg atcattgggg
     1201 ttgattgcca tatcaatatc tccaaaaatt acttgaccaa tnnnnnnnnn nnnnnnnnnn
     1261 tgtattgtgg ccaacggtca tgacatcact atgtcagttt tatgtcaggt aattaaagtg
     1321 tcacatttac gtcatgggct tcttgtaatt tacttagtgc aaaggagctt tcaatgaatc
     1381 agaatcaaga tgatctatca caatggtaca aaagagccca agatgagatt ttggatgacc
     1441 tcgatgagga attagagctt gagcttgatg atcagcgctt gggggatgac tcaagttcat
     1501 taacagcaat taatcgtcag ttttatttca aagaactttt tcgtcttcag aaagagttga
     1561 ttaagttaca agattgggtg gttcatcata agttaaaagt tgttgtttta tttgagggtc
     1621 gcgactctgc tggaaagggc ggcgctatta aacgcattac acaacgatta aacccccgcg
     1681 tatgccgagt agttgcccta tcagctccat cagagcgaga gaagacccag tggtattttc
     1741 aacgctatat tgcgcattta ccagctgctg gcgaaatcgt attattcgat cgcagttggt
     1801 ataaccgagc aggtgtggag cgagtgatgg gcttttgtag cgatgatgag tatcaggaat
     1861 tcttgcaaac tgtcccagag tttgaaaaaa tgattattcg ctcaggggtg attctaatca
     1921 agtattggtt nnnnnnnnnn nnnnncttaa gcaatggaaa ttaagtccga tggatttaga
     1981 gtctcgccga cgttgggagc agtacaccaa agcaaaagaa atgatgcttg aaaaaacaca
     2041 tatttctgga gccccttggt gggtcgttgc agcgaatgat aaaaagcgtg ctcgcttaaa
     2101 ctgtattcat catttacttg ctcagattcc gtatggggag attgagcatc cccacattac
     2161 gctacctgaa cgggttcata acccagacta tattcgcggt cctgtccccc gcgaaatgta
     2221 tgttcctgaa atttattaat cactaatcaa ttggtacacc atgaacgaat ttgataatcc
     2281 catcgttaat actctttttg atccccaaaa taccctcgaa accaaaacgg accgacgctt
     2341 ttttctaaag tcaagcgcag cactcttggc tgtaatgagc cctggtcttg gcatggcgca
     2401 aagtactaaa tcgatttggg gtggagcgaa gccatttaat tttgagtctg tctcactggg
     2461 gcaggcaagc gatggcattg cgattccaaa aggttatcga tgggcaatag ttgctgcatg
     2521 gggcgatccc attaatggta agtttccagt tatttcctat gatgtgatta atacgccaga
     2581 gcaacaagcc aagcagtttg gcatgcacca tgatggctgc gcatttttcc cagagcgggg
     2641 ttcatccaca aagggtcttt gggttgttaa tcacgaatac actgatgatg gtcttctgca
     2701 tcctgatggc atgaaaacct ggaacgctga taaggtgcgc aagagtcagg cagcgcacgg
     2761 agttacagtt gcccatattc agcgcgagag cagtggtgct tggcaagtag tatctggccc
     2821 aaatacaaga cgcattacag cctatactcc ctgcgctatt tcaggaccag cggctggaag
     2881 tgtttatatg caaacagtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn ntacttgacc tgtgaagaaa atataaatgg ttattttgtc aagaagggga
     3001 aggctagcaa ggaagagcag cggattggta ttaatgcaaa ggggtttggt tatcgctggg
     3061 aggagtttga tgagcgcttt aatactgacc ttacacccaa tgagccgaat cgctttggtt
     3121 gggtcgttga aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtgtagt
     3241 ggtctacatg ggcgatgatc aacgcggtga atatgtttat cgttatgtga gtaaatttcc
     3301 ttacaaacca aatcagccag aacacaatag gacgctctta gatgagggca cacttttcgt
     3361 tgctcaattt tcggatagtg gcgaaggtcg gtggatcccc ttggtccatg gtcaaaatgg
     3421 cttgagtaaa gaaaatggct ttccagatca agcgtatgtg ctcatcgaag cgcgcttagc
     3481 ggctgattac ctgaaggcaa ctcctcttga tcgccctgaa tgggtagccg ttcaccctaa
     3541 aaccaatgac gtctatttgg cgctgaccaa caataatcaa cggggtaaag agttccccgt
     3601 aaatgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     3661 tgattgggag atttttgtct tggcgggaga cccagacaat accgatctaa atcagaaagg
     3721 gaatattcgg ggggatgtct atggatcacc tgacgggttg tggtttgacc agcgcggaat
     3781 tttatggact caaactgata tttcttcagg agctttgggc aaaggcgcgt attcacgcat
     3841 tacgaacaac atgatgtttg ccgcagatcc agttagtaga gaatttagac gctttttaat
     3901 tggacccaat ggttgcgaag tgaccggtgt tgacttaaca cctgattaca aaaccatgtt
     3961 tgtcaacatt cagcatcctg gtgagagtcc gagcgagcgt agtaatccgg acaagccaaa
     4021 agccttcagt gattggcctg atcgcaaact gttttccaga ccccgatctg caaccattgc
     4081 gatttggcgg gaggatggca aaccggttgg tagctaatta gggttgaatg aggaagggtc
     4141 gaaatgaaaa ggggctttgt tttgttttat ctacagtaat ttcgaccgct tcaatttgtc
     4201 gatcccccat gacttgcaaa cggtaagctc gatcaagagt acgcttacta attggatcgc
     4261 taaagggttg atcaatgatt aagcgatgac tatcgccatg aataataaga gcaggtttgg
     4321 caaagtccgc cagtttttga ttcagtagag caatcgtatc gcgtaagccg ctagtnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnntaa attggcttga ttacgtcggg tatattctgt gaactgacta
     4501 aagttattgt ttgaaccaac aacgtggacc gtgacaaata gcgtggactc gtggaaccag
     4561 taggcattct caacaaactc attaaacctt ggatgaatgt ccgcctgacg atttaagcga
     4621 atcgggtttt tgccaagact cttggtatcg gaaaaataga gttgacgaat aaattgcagt
     4681 cgctcgcttg gttcaaatgc tccagcactg gggcgatgac aatcagtcca ttcattatca
     4741 ccgggtgtat aaaccagtgg ctgattaaag gtattaaata accgatgaat tttctgtaaa
     4801 tattcattgg agcagggcga actaccattt ttggtgtcac ccacaaaaat gctcaatgtt
     4861 ggctgtgacc gattaatcga ttggattaat tgctcaaacc ggatctcgtc cccatcgcga
     4921 tagggcatat cacccagagc gataaaccga aatgcgtgtg cccagagtgg aatcatgcag
     4981 cacagagaag ctactactat ctgccgcagg agagtcttaa tgggattcat cacagtgaaa
     5041 tattatccat tcagaatggg attatgttaa ttcaacaatc aaccttaacc tgcccacatt
     5101 gttttgttcg tgaatgtgtt gaaattcatt cggcgtatcc ataccaattt catcaatgcc
     5161 catcgtgcct tcgaccctat caagccaata aaaacgattg ctgtattttc tgtagctacg
     5221 gcgatacacc atgcaatcaa atcgatcgaa acttagctct ataaaaaaac cgccctagaa
     5281 gggcggagag ttcaacacgt agggagtttt ttttaacttt atacaatcct aacgtgacag
     5341 cttgatggca gttttgtgtc agctctaatt ggtgcaatgc aacaattctt tgtcatattc
     5401 ctttgctaga ctgatttttc attaattgac atgaggtgtg tgtaatgaaa aagccgtcag
     5461 aagcagcaaa gaaagcgaag gcaaaagagc tctttactct gtctcataaa ttgcttgatg
     5521 cagctaagca gctcagcgag caccatgcag cagaacttcg ctacaacatg cagcaggcga
     5581 tggatgccgc taaaacagcg gctcaaaaag atatgaagca actcaatgca atgcaaaaga
     5641 aggcagcnna aagatattgg tagtgaggca gcagatggtg ctgagaagca ctttgagcgg
     5701 gctcgagacg ctattagtga ttggattaaa gaggctgata agaaaatccc catgggcgga
     5761 gaaaaactcg cgaaagtcgt gcgtgaggtt tccgatgcgg gtgccaaagt atttaaagaa
     5821 ggccgcaaag cagtaaatga tgccattgag ttggccgaga agaatatcca gacggttgcc
     5881 aaaaaggtga gcaaagccac ggctagcaaa aaggccagga agcccgctca aaagaaactg
     5941 gtaccccgca aatcgacggc tgtgaagaag aagaccgccc ccaggaaaaa gcccacaaca
     6001 accccaagcc aggcttcaca aagctctgtt ttaggaaatg gcagcgccgc agtacctaac
     6061 tgagttgcta aataagggaa ttcaccctaa atatttaggg tgaatcatga tttttagcta
     6121 cagagcagcg gcaacaatta cttaacctat tgatttatat aattctttta gattaactcc
     6181 tgcatcgttt cacctgtcac taaagcgtca tattcccccc gtattgttct ctcatcgcgt
     6241 tgtgcgagac cttattgagg attaaaaatg acgattacct tgaaaaaagc ccttgcggct
     6301 ctttccattg ctactgtgag ctttgctcca gctgcgtttg ctcaagaaat taccggtgct
     6361 ggtgcaagct tcccatttcc aatttacgcc aaatgggctg aagctcataa agcaaaaaca
     6421 ggggtaagtt tgaactatca atcgattggt tcatccggtg gtatcaagca aattaaagcc
     6481 aagactgttg actttggtgc aaccgataac cctgtgaaat ttgaagattt acaagccgat
     6541 ggcttagttc agtttcctgc cattattggt ggtgtagtac ctattattaa cgttgatggt
     6601 atcaagcctg gtcaaatccg tttggatggc caaactctgg cagatatttt tcaaggcaag
     6661 atttctaact ggaatgacaa gcgtattgct gatctcaatc caggcgtgaa gatcccttct
     6721 ggtgatatca cgatcgttca ccgcgcggat ggctcgggaa cgaccgcgat tttcaccgac
     6781 tatttagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngttgagtat
     6961 gcatacgcca agaagaacaa catgacccat gtgcaattaa gaaaccttgc tggtcaatat
     7021 gtacaacccg atgacgacac ttttgctgcc gcagcggcag gtactgattg ggcaaagatt
     7081 ccaggtatgg gtacatttat taccaatgct gctggtgcga agtcatggcc tattactggc
     7141 gcatcattta tcttgatgta caaagagcct ggtaacaaag ctcgctctgc
//
LOCUS       DDPQ01000129           14417 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11364,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000129 DDPQ01000000
VERSION     DDPQ01000129.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 14417)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14417)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14417
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11364"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3285..3323
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3638..3665
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5753..5763
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7249..7281
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7446..7498
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9620..9671
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10210..10241
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13892..13915
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3443 a         3620 c         3324 g         3758 t
ORIGIN      
        1 tataccgaga tgcttaaccg cgctgttaaa tcgctgcgca gtggtaagga gcccgacctc
       61 ctatcaccga tgcaagcaac aaccgatgtg agcttaggtg ctcccgcctt attacccgcc
      121 gattactgtc cggatgttca cgaacgtctc tcgctttata agcgctttgc cagttgcgaa
      181 gactttacag acttaagttt cttgcgagag gagttaattg atcgctttgg taacttaccc
      241 gatgcggcta aagcctttta tgaaacccac cgcttgcgtt tagagatgag tttattgggt
      301 atcaagaaaa ttgatgcgag tactacagct attcagctgc agtttatacc tcagccacca
      361 ctcgatccgg tcaagattat tcggattgtg caatccaatc ctgccattca tctaaacggt
      421 caagatcgga ttaagtacct gcctcccaag gacgcttctt ttgagaaact agagcagcgc
      481 attgaaggta tccggcagct catgaaatta ctcagtagcg ctttggtcgc tagtgctcaa
      541 gccttgccta caccggcact ttctgcataa tgcaatcatg gcaaagtctt ccccccatcc
      601 ttttccagta ctccaagttt tagccccagg gcttctttcg tcagaggtct tgatcggtct
      661 tgagaaagcc ttggtgaagt tagaaattgc ttacacaaaa gtagagcagc gttttttact
      721 tggtagagag cttgagttat ttgagcgcaa tggcctgcgg cagttttgtg cagagcgttc
      781 tcatgacctc gcaatcttgc cagctcagtt ctcggccgat gctttacagg tattggcaat
      841 ggatatggac tctaccctga ttaatatcga gtgcattgat gagatcgctg actttgcagg
      901 taagaaagct gcggttgctg agatcacggc tgccaccatg cgtggggaga ttgttaattt
      961 ctctgagagt ctctctaaac gcgttgccct cctagcaggt gttccccaga ccgccctgca
     1021 ttcggtctat gagcaacgtt tgcaactcaa ccccggtgct gagttattga ttgcgggggc
     1081 acatgaacgt catatgcaca ccctgttagt atctggcgga tttaccttct ttacgggtcg
     1141 tatgcaagaa cgcttacatc tgagcgagac ccattcgaac gaactcgaga tcgtcgatgg
     1201 agtgctcacc ggtcgagtct tgggtgagat tgtggatgga acggccaaga atcactacgt
     1261 tatctctgct tgcactaccc tcggtctcac caaaaagaat gcgctggtga tgggcgatgg
     1321 cgccaatgat ttgttgatga tggctgatgc tggcttgagt gttgcctacc gtgcaaagca
     1381 agtggtcaaa gaaaaagccg acattgcact tgactttgtc ggcttagatg cagtcttgca
     1441 actgctatag caaattagat tcttgtggtt tgatcttctt ttagatcttt tcaaagatcg
     1501 cagcaatccc ctgcccgcca ccaatacaca tggtgaccag gcaatactta ccgttcgtgc
     1561 gatgcaactc atgaatcgct ttggtggcaa tcgctgcgcc agtacaacct acggggtgac
     1621 caagcgctac tgcaccaccg ttcacattgg tcttggcagg atctaatccc aaaccttttg
     1681 taacggcaat cgcttgagca gcaaacgctt cattggactc gatcacatcc atcttatcga
     1741 gtgttaaacc agcgcgttgc aatgcaatct tggttgccgg gatggggcct tcacccatga
     1801 tgtgattagg tacgccagct accgcatacg agaccaagcg tgcaatcggt ttttgaccag
     1861 ccttggcagc cacatcagca gctgctagca ccataaatgc tgcaccatcg ttcacacccg
     1921 atgcattacc agccgttacg gtacctccct ctttcttaaa cgctggcttc atctttgcca
     1981 gggtctccat ggtggtatcg ggcttgcaat gctcatccgt atcgaacacc acatcgcctt
     2041 tgcgggtctt gatggtaatt ggcacgatct gtgatttaaa acgaccttct ttaatagcaa
     2101 gcgcagcacg acgatgtgac tcgactgcaa acgcatcttg ctcttcacgc gtaatcttcc
     2161 acttttctgc taagttctct gcggtgatac ccatatggcc caccccaaat gggtctgtga
     2221 gaaccgagac catcagatcg atcatcttgg tatcgcccat ccgcgcacca ttacgcattg
     2281 cggttgcacc atacatagcg cgagacatca cttcaacacc gccaccaaca ccatagtcat
     2341 agtcattgag catgatgcct tgggcagtgg tcacaatagc ttgcaaacca gagctacata
     2401 aacgattaac tgccatcgca accgagtcca tcggcagtcc tgcttgaata gaggctactc
     2461 gggcaacata ggcatagcgg ttatcggtag gcatcgtatt accaaccgtt acatagttaa
     2521 tcttggcagg gtctgcacca gatcgggcga ccgcttcttt catgaccgta ccagcaagct
     2581 cgcagggctc aacactgctt aagctgccac cgtatccacc aatcgcagaa cgggccgcac
     2641 ttaacactac aacgtctcta ctcatcatca ttcctctcat ttacattagc cgttgaccag
     2701 tcatattagc ttaatagcta gttttctaac attatctaca cttccacaat cagatcgtgt
     2761 ccttgcgttt ggattacggt cgctttaata aagtctccta cccgataacg cttagagggc
     2821 ttactggggg gtagtatgcg tactaatccg tcgatctcag gggcatctcc ggcgctacgg
     2881 ccaataccac cctgatcgtc cagacggtcc accaagatcc gaatccgttg gtctactaag
     2941 cgcttaatcc gcttggtcga gaccgtctcg gctacctcca taaagcgggc ttggcgttct
     3001 tcccgtattg cttgcggaac cggatgatcg agctcattgg cagcagcccc ctcaactggc
     3061 gagtaggcaa agcagcctgc tcgatcaatg ttcgcctcct gcataaagtc taggaggtac
     3121 tgaaactcct cttcggtctc gccaggaaag ccagcaataa atgtactgcg gatcacgagg
     3181 tctggacata cggcgcgcca ctgctcaata cggtctaggt tcttctcgcc actcgcagga
     3241 cgcttcatgc gtttgagtac gtcaggatgt gcatgttgca tgggnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnntcgacgt gtggataggg atatacataa tgcaagcgta
     3361 cccaggcttg gtgctcttta gccaattgat taagggctag agctagatcg tacaagcggg
     3421 tcttcacagg tttgccatcc cagaaaccag ttcgatactg aatatcaact ccgtaagcgc
     3481 tggtgtcttg cgagaccacg agtaactctt ttacgcctga ctcaaacagt tgcttagctt
     3541 ctttaagaac atcgccaatc ggtcgtgata ctagatcgcc acgcatactg ggaataatgc
     3601 agaagctgca ccgatgatta caaccttctg agattttnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnntttgg taaatgcgca tggactgctt gtaatacctc tgcagtggca tggggtccgg
     3721 tcacagctaa gactttagga tggatacttt taacaagaac gctcccatcg ggattttttt
     3781 tggcacccag gcatccggtc acaattacct taccgttttc agctaatgcc tcaccaatgg
     3841 ctgagaggct ctcttccaca gcagagtcga taaagccgca ggtgttcaca atcacgaggt
     3901 ctgcaccagc gtattctttg gcggtctcat aaccctcagc actgagttgg gtcaagatca
     3961 actcagaatc cactaaggct tttgggcaac ctaatgacac aaatccaact tttgcaacca
     4021 cgccaaatgc cctatctatt tatgaaaaag aaataatacc catgcgcagg tgcgctgggt
     4081 tttgttctaa gaatccttgt cggagttctg tgtttttgaa ttaaacggaa atccagtaaa
     4141 taaagtgttt gatccttgca tttgctcttg catttttaga aagagatctt tgctctgatc
     4201 catatagctc gacatcaaat tttgcataaa ggggttttgc aagttcatca tttgggtcca
     4261 ggcttcggta ccaccaccgc tcatcgtttt actttgatcg tttaatttct ggtgcatatc
     4321 cataaaggat tgcatcgtac gctcaagatg attacccatc agaccttgca tcgagtggcc
     4381 ataaaaacga atcacttgct gcaacatctg ggtcgagaac actggcaccc cacaggcctc
     4441 ttcttccaaa atgatttgca agaggatgct gtgggttagg tcttcatcgg ttttagcatc
     4501 gactaccttg aaagcttccg ccgccatgac caactcttta atatcagaca aggtgacata
     4561 ggcactcgtc tgggtgtcat aaagacgccg gttcggatac ttcttaatta accgttcttc
     4621 gccagccttc ttggtacgtg atgacatggt ttcccttgct atatggtgca atgcagaatt
     4681 taccctagtt tagcctgtat ggataccacc attgagggag aagtctgcgc cggtagagaa
     4741 tgcgccatca tccgaggcaa tccaggcaca gattgaggca atctcatctg gggttccaag
     4801 acgcttgact ggaatggtgc caatgatctt ctccagcaca tcttcccgaa tcgctttgac
     4861 catatcggtt gcaatatagc ctggggagac tgtattaacg gttacacctt tgctagctac
     4921 ctcttgcgct agggccatcg tgaacccatg caaacccgcc ttcgcagtgg aatagttggt
     4981 ttgtccaaac tggccttttt gaccattcac ggaggagata ttaatgatgc ggccccagcc
     5041 gttatcgatc atgccgtcga tcacctgttt ggtgacgtta aagagggagt tcagattggt
     5101 atcaatcacc gccttccaat cctcgggggt catcttacgg aatacgctgt cacgggtaat
     5161 gccggcattg ttgaccagta catcaacacg gcccacttca gccttgacct tttcaaaggc
     5221 ggctttggtg ctctcccaat cggatacatt accttccgat gcaatgaact cataaccgag
     5281 tgccttttgt tcgccgatcc agcgatcctt gcgcggtgag tttggaccgc aacctgcgat
     5341 caccttaaat ccaccctttg ataaacgctg acagattgag gttccgatac cacccatacc
     5401 gccagtcaca tatgcaattc gttgagccat gatttactcc ttgttattgg tgtgatcgat
     5461 caaccactta aaccttttgc gccttctctt taacgtaggt gccaggcgcg gcaactaatt
     5521 ttttatactt cccaccaccg tattggctgg gggcgactac tttcttacca ccgtactcgg
     5581 ccaaccactc ggtgtaatcg ggccaccaac ttcctgcaac ctccttggca ccttttagcc
     5641 attgctctgg ttttttgggt aactgagaat tggtccagta atgacgcttc tttttatggg
     5701 gcggattaat cacaccagcg atatgaccag aagcgcccat cacaaaacga atnnnnnnnn
     5761 nnntcgcgcg atgcaaatac atatgctggg cagttaatct tagtaaggtc aaccgactca
     5821 ccacagacct tcagtacccc aggcttagct agatcatttt gcaagtaggt gtgacgcaaa
     5881 taccaacaat acatcgggcc tggtaagttc gtggagtcac cgttccagta gagtagatca
     5941 aatggtggcg gtgagttacc cttcaaatag ttgtccacta cgtagttcca gaccagttca
     6001 ttgggccgca agaaggagaa ggtattaccc aactccaacc cagagagaag tccgtagcgc
     6061 ccctctgctc caccaatagt tttctcacgc agcttaacca aagactcatc gatgaaaaca
     6121 tcgaggatcc ctgtatcagt gaagtctagt aaggtagtga gtaaggtgag actagcgatc
     6181 gcttcttgct tgcgtgccgc taagaccgct agcgaggagc tgactaaggt accgccaaca
     6241 caaaatccca agatattgat cttgtcttgc tgactaattt cttgggtcac ccgaatcgct
     6301 tctaagaccc cctgaccaac gtaatcatcc caagtgattt gattcatgct ggcgtcggca
     6361 tttttccagg agaccaaaaa caccgtatgc ccttggctca ccatatagcg caccaccgag
     6421 ttatcgggtt gcagatccag aatgtaatat ttattaatac acggcggcac cattaaaaat
     6481 gggcgctcat agacctgatc ggtcagcggt ttgtattgaa tgagttcaaa gagtttgttc
     6541 cgaaacacca cagcaccttc ggtttgtgcc aagttctgac caacctcaaa gccactctca
     6601 tcggtctgac taaccttacc tttacccaaa tcgcctaata aattaacgat tcccttttgc
     6661 agagactgcc cctgcgtttg caagatggtc tctaaggcct caggattggt ggcaaagaaa
     6721 ttggcgggcg ataaggcatc aatcatttgc tgggtcgtaa actcaatgcg ctgcaaggtc
     6781 ttggggtcgg tttctactgc attcacgagt tcattgagat aacgtgagtt gagtaaatag
     6841 gtggacacca atgctttact ccaagggttt tgccaagcct gcccttgaaa gcgccgatct
     6901 ttaatctcga gggcatcagg atccttgcct aacttaccca aatcctcaag gtattttttt
     6961 tggatctctg aaagttttac aggtggaatc atcgccatgt attgcggtgc tagtgttggt
     7021 gaatttccca agttcataat cggtgcaatc tttaagcaat atgtaaaacc aaaaagtaca
     7081 gccctattct cagacgatta ttgctctagg gttataagca ctaaccctag ccctaatgag
     7141 gttttgaggg cgatctattc ctcaggaccc ttgattctaa ggctttgggt taaaccagat
     7201 caggctggca aagcgcccgg tggggttttg acgacggtag gagtaaaann nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn ngcgtgtaga cgttcttttg caattctagg taaatcagcc
     7321 atatagcgct gggcattcat actcttctca aaacaagaat cactgaacgt atgtcctctg
     7381 tgctcagctg cccttaagaa ggcatcccgt acttcacttc ccacctcata gtgctttaca
     7441 gaaatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     7501 gttctcaagc actccctcac acaatccacg ccagcccgca tgtgctgcgg caatcgctgt
     7561 acctgcttgg ttagtcaaca agaccgacat acagtctgcc gccaagatac ttaatactgt
     7621 ttggggtgtt tgggtgaacg aggcatctgc ctcgaccaca ttctcatgaa tacctggatt
     7681 gcttccctgc accttaacta ctcgggtgcc gtgtacttgc ttaatccaat aagggctttg
     7741 agggagcaag gcatctaaga ccctacggtt ttgcagaact gcttcgagct catcaccagc
     7801 gtaagcaccc agattgaaac catcaaaggg gggtttgcta aagccgccgt gacgagtggt
     7861 cgtgaacgca tgaacatgac tgggtgctgg ccattgagcc cgaatcactg tgagcgcgct
     7921 catctaatta gcctcagata atgcactgga tgctgcagtg ggcaagaccg cttcatttaa
     7981 ttgtactgcg ctatttaatg ccaagagatc cgcaggcact ggggcaaacc agctcatggg
     8041 cttttgggtc ttagggtgga tgaactgcag tgcaaaggca tgcaaagctt gtcgatgcaa
     8101 gttcagttcc ttggccgctg caggtatctt tttgcgatac accggatcgc ccaagagggg
     8161 gtatcccaat gactcaagat gaacacgaat ttgatgggtg cgaccagttt ctaagcggca
     8221 ctctaataaa gagaccttgg cagtccccat cgatccaagt gctaagcgtt tgtaatgggt
     8281 caccgccgct tttgcgcctt gctggcttgc gctcaccacg gccatcttta aacgatcacg
     8341 ctggtcacgg cccacggcgg catcaatcgt gccacgcaat ggagtatcgc cccagaccca
     8401 tgccaagtag cgccgttcca ctaaatgatc ttgtaataaa cgcactaggg ctgtttgtgc
     8461 aatcgcagta cgtgccacca caaataatcc cgaggtatct ttatcgagtc ggtgcacaat
     8521 gcctgcgcgc ggtagatgtt gtaactccgg gtaatgaaag agtaagccgt tcagaacggt
     8581 gccactccag ttaccagcag ctgggtgcat caccattcca gctactttat gaaagaccag
     8641 gatcgtgtca tctgcgtaga tgcaatcaag tgggatgttc tctggcaaaa aggcatgttg
     8701 ctccggcatc tcctggggaa agacccgaat ttgctcaccg ccacgcagga tttggcgagc
     8761 cttgaccact ttgccgtcta ctaacactgc tccctgggtc gcccaagctt gtaaacgatt
     8821 acgagaatag tcactcaaaa aatgagccaa tgccttatcc aatcgctcac cggccatatg
     8881 gtctggaatc tctagagcaa tgaaatcctc atcatcgata taatcagagg gattcggatt
     8941 aggagtttgc ggcaatgcca cgcttgaagg accttattgt gttgaactta cccaccgtgg
     9001 ggcgccatct tgctagtgta aggcttgtcc agcttaccct cctgatttgt gctggcgcgc
     9061 tcttgagtgc ttgtgcaaat accaataccg atgaaactgt aggctggtct gaggccaaac
     9121 tctttaccga agccaaagat aagatggctg agggtgactt tgataaatgc ggcaagtatt
     9181 ttgagaaact cgaggctcgg ttcccctttg gtccttactc gcaacaagcc cagattaact
     9241 cggcctactg tttctggaaa gcgcaggagc ctgctcaagc cctcgtgcaa attgatcgct
     9301 tcattaaatt acaccaaggt aaccctaatc ttgattacgc ctattaccta aagggcttaa
     9361 ttacctttaa tgatgatctg ggttttatgg gcaagctcac cgggcaggat ctgagcgagc
     9421 gcgatcccaa agcagccaaa gaagcctttg aggccttcaa ggtcgtggcc gagcgattcc
     9481 ctaatagtaa gtatgctccg gatgcgattg atcgcatgcg ctatatcgtc aactcactcg
     9541 ctgaggccga tgtcattgtg gcgcgttatt actttcaacg cggggcttac ttagcctctg
     9601 ccaatcgcgc tcagttggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn ncttggaatg aatgacctta gctccgacgc aatgcgtgtc tttaagctga
     9721 acttccctga tagtcagatc tttaaaaccg gcgttcgggt tcagaaagaa cgcaggtggt
     9781 ggcagttctg ggtgactaaa taatctcgac gggtactcga ggtgcgatcg cacatacgag
     9841 ctcataccca atcgtttgtg cttgattagc gacatcgtct accggtagat ttttacccca
     9901 caactccacc gaagtgccaa gcgttgccca cggtgcatga ctcaaatcaa tcgtgagcat
     9961 gtccatcgat acctgcccgg ctatggggca gataattcct gaggttttat cagtgggtcc
    10021 gctgacccaa acaggagtgc cgtctacggc atgtcgtgga tagccatctg catagccaca
    10081 cgcaatcacc ccaatgcgca ttgcctgttt ggctacataa cgcgctccat agcccactgc
    10141 atcccccgct tggagctctt gtacagcaat aattttgcta gagaggctca ttacggtttt
    10201 aagatgagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtcttacc caatcgccta
    10261 gggcatttcg atgccaaagt acggcggcag agtttgctaa ggaggtctcg ccctgaaggc
    10321 catcaatcgt tttgctaaag cattccattt gtgcaccaac agaaggctca cgatcgaccc
    10381 gatccgcatt ggcaaaatgg gtcatatgat gaacgtggtg accttgcgca tgcaaacggt
    10441 ggtaggcaag gcggtaggcg tcgggggtga accctaaacg gttcattcca gagttgagtt
    10501 ttagaaacac ggtgatggga tggctttgct tctctagcca actaacttgc tcggcacaat
    10561 ggaccactag atcacaccgg agctcaatga ccgagctgag gtcatcctca ttaaataagc
    10621 cctctagaag caaaatgcgc ttatcccagc cttgggctct tagccaacgg gcatcatcta
    10681 gatcaaggag tgcaaagcca tcggtttggc ctaggccggc caaggcagcc ttcaggctat
    10741 gcccataagc acgggcttta accacagacc atatccgaga attaccaact aactcacgga
    10801 ctcttgccaa gttgtggctt aatgcacctg tatcaatcga tgcgagaatc gggcgctcca
    10861 gaaaactccc ctttcatgct ctaatctagt aaatgaagca attgtacgaa tgaaacctgg
    10921 tttttacact attatggcgg cgcaattttt ttcgtcgctc gcagataatg ccctcctaat
    10981 cgcggcgatt gccctattgg ctcagcttgc tgctcctgcg tggatgaccc cactccttaa
    11041 gctgttcttt gttttatcgt atgttctctt agctgcattt gtggctgcct tcgcagactc
    11101 tcgacccaag ggtcaagtca tgttcatcac caacaccatc aaaattgtgg gttgtgctgc
    11161 tatgttattt ggggcgcatc ccttagcttc ctacgccatc gttggtttag gggctgcggc
    11221 ttactcaccg gccaagtatg gaattttgac cgaactcctg ccccccgaga aattggtcgc
    11281 agcaaatggt tggattgaag gattaaccgt agggtcgatt attgcgggca cagttttggg
    11341 tggggtatta attagcacta ccgtctccca caccttaatg agctttgact taccaacagt
    11401 taccactggg gtggatagcc ctgctgagtc cgccatctca atcatcatgt tcatttattt
    11461 gattgcggca ctctttaatt tacgaattcc cgatacaggc gctcgctatc cagaacaaaa
    11521 gattaatcca gttcagctca caaaggattt ttttgtaggc tttcatgtcc tttggcgtga
    11581 tcgcttaggt cagatctcat tagcagtaac caccctgttt tggggtgccg gtgcaaccct
    11641 gcagttcatt gtgcttaagt gggcagagac cgctctcaac atgaacctct cccaaggcgc
    11701 tatccttcaa gcagtttcgg ctattggtgt tgcaggggga gcagtttggg ctgcctcgcg
    11761 gattccattg aagtctgccc tgaaagtttt accttatggg gtggtcatgg gtttattggt
    11821 ttgtgtcatg gccatctacc atattgaata tctcccctcc tttgccttgt tcaaggttgg
    11881 cgactttgta gtgaacttca atctcctgcc agcctatgta ttgctaatta ctgtgggttg
    11941 gctggcgggt tattttgtgg tgcccatgaa tgccctctta cagcatcgtg gtcacgtttt
    12001 attgtcagca ggtcactcca ttgcggtcca gaactttaat gagaacctct cggttctcat
    12061 gatgttaatg ctctatgcct tattaatttg gcttgatgtg cccgtaccga ttgtgattac
    12121 aggctttggc attacggttg cggtcaccat gtggttggta atcaagaaac atcaggccaa
    12181 tcaagctgag tatgactcac tccacttaat tggtgagtct aagcactagg tttagagaga
    12241 ctgcagtacg gagcgcgcta agagtaggtc ctgccaggca agtgccttat cagctggctt
    12301 ctgtagcaga tgggctagat caaagcttgc caccagaggt atatccagtc caccgagttc
    12361 tagaacaaca tcccgtaggt tctctaagag ttcatgctcg ccacttaaag attgcgccgc
    12421 ctgttgacca aacgcaaatg caatacaagg taggctggta tctggcgctt tagtattgtt
    12481 aggttcacgc cactcccact cactttgtaa taggccaaga gcacgaacga tgttcttaaa
    12541 caatacttct gcatcgccag aaggcgcttt gccataaaac agccaataca ctttgggtac
    12601 caagtttgtc ttggtttggg ctggcgtttc tggggccgaa gcttgctctt ccttagtaaa
    12661 actaggacct gtctcgcgta aggaccattc gctaatgccc atttctttta agtagatcga
    12721 ggcttgttca gtgctcatac agattggcgt gttgcttcag tgttttagtc atcaccagcg
    12781 catcctcacg ctgacccgtg gaggattcta agggatagta ggctttgcga gttgcgagtt
    12841 cggtaaagcc catcttggta tagagagcct ttgcagcaaa attactgggg cgcacttcca
    12901 atagaatgcg tgggatttgt aaattctggg caatcgactc aatcgcttgc atcaaacgca
    12961 gtgctagtcc ttggcgccgc atacttggat tgactgtaat gttgagaaga tgcaaatcat
    13021 cgactgcggg aagaaggatg caataggccc aaattgtttt tggctcttca ttggcctcgc
    13081 gcatgcaata cgcccagtgc ccagcggcaa tcgagtctaa gaaattacct ctgctccaag
    13141 gatgggcgtg cgatgcatac tccatcagca gaacctcgtc gaggtcagca tcactcatca
    13201 actctagcac taactcagcc aacagaatga ccggttgggg tactgtgtgc tgcagcagat
    13261 cgttcttcag tggtaagagc taccttgttc cgaatataca aaggctctag ttgctctacc
    13321 gcaatagttt gtttagcctc tagtaggggc ttcgcccagt ccaagatacc aagggcattg
    13381 ggtaccagtt gagtatccat acgatccttt aatggtgatg actcaaggag tttaggatac
    13441 tcctggaatg cattgccggc cgcatatgca agaccatcaa ggttaatact ctcggggcta
    13501 cttaaggcaa tctctccaat gcgcttggga gtctgattag aaatgggatt aacgtgatag
    13561 cgtgcccaat acacctctcc catacgggca tccaaggcaa ttaaaaactg agtatcggct
    13621 gctggtgagc ttctttggaa tgtattgctc tggaccactt gcatcgccat ggcatcaaga
    13681 ctagctacgg gtattacggg tcgtttggct ccgactgcta agccctgcgc gatggcgacc
    13741 gatagacgca cacctgtaaa tgcaccgggg ccaatcccca ctgcgattgc atcaagatct
    13801 tgatattggg cgtttaccga atggagtagc tcattaatcc aaggtagaag atgttggcta
    13861 gcacctgcac caatgagttc atggcggtgt tnnnnnnnnn nnnnnnnnnn nnnnncgcat
    13921 gcgagcagaa ctagaacatg atgctctagc tgttagaact cttcatacaa tgggagggtc
    13981 aggaactcta caaactgatc attggcaacc agatcatcaa agatcttggc agcgcggtca
    14041 aactggccga ctgcaccaga gtccttcact ttgttgagct cttccccaat cattttgcgt
    14101 acaagctcga tcgtgacctt acgtccatcg tctaagacac cttttggaga acggatccac
    14161 tgccaaactt gcgagcgact aatctcagca gtagcagcgt cttccatcag attatgaatc
    14221 ggtacgcaac cattacctgc taaccaagca cccatgtaat ggatacccac attaatgttg
    14281 taccgcaagc cagcttccgt aatggggccc tcgggtgcaa aattgaggat gtcggcagcc
    14341 gttacctgaa tatcgtcacg ttgacgatca aactgattac ccttgtcacc caatacggcc
    14401 ttgaactcgt tcatgca
//
LOCUS       DDPQ01000130            5219 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_2702,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000130 DDPQ01000000
VERSION     DDPQ01000130.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_2702"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    4788..4896
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1200 a         1359 c         1247 g         1304 t
ORIGIN      
        1 ctgttatgct catgaaatcc caccaagacc gtacccttct taatcgagcg atcgatcaca
       61 ctcaagcgtc ccctgtggcg atactcccaa gccggtcctg cgataggtcg catgatctcc
      121 tccgctttta agcggccgat atgccacaga tcatcttcta gaacccgttg cttgatagca
      181 atttgcgcag caaagtcgag gtgttgcatg ctgcagccac cacagatgcc aaacactttg
      241 cactttggct gcgtgcgata aaccgctggc ttaatcaatc gttggagctt ggcttttgca
      301 aagcgggcct tatcccgggt gaggatgtag gtcacatgct cagtgggtaa ggcgcctcgg
      361 ataaaaacaa ctttgcccgg ttggccgttc tcgtcgctta gcctggtaat gccttgggca
      421 tccagatcaa gggcttccac aataacaacg ggagactctt gatgtgaaat tgatcttggg
      481 gttctggtca caattgactc aggctaaaaa cagggtcatg caatctgatg cttattgtgc
      541 aaagccagca agatactctg gccaaggtgc tttaccctgc tttgcctcct gagtgagttg
      601 ctgtttaaca aaatcaagct cctcttcata ctctgcatgc gagaaaccgc cacgcatctg
      661 ttggaagcgg caatacacaa gataggtgtt cacaacatcg gtctcgcaat attgccgaat
      721 ctgctcgagt tgccccgctt ggtatgctgg ccatacttga cttccatcca ttcccatttt
      781 tccggggaag ccacaaagtt ttgctaaggc atcaaggggc gcattggcac gaccattatg
      841 cttagcgagg acatccatca aatcaatatg acgcatgtga taccgattga tgtagttgtt
      901 ccacttgaaa tctttactat cactatcgtt actctcgccc atctcccaat agcgcggcgc
      961 tgcaacttga ttgagcatcg cgcggtaatg caagaccggc agatcaaagc cgctgccatt
     1021 ccaagagact agttgcgggg tgtatttctc aatcagctca aagaatgcct ggatgattgt
     1081 tttttcatca tccccaattt ggccaagact gccaaccttg aattgagggg taccgtcttt
     1141 agtggttctg cgaatgacgc aggagattgc gacaatcttt tgtaaataca gcggtaagaa
     1201 atcactacca gtttgtgcag cgcgagcttc catagcggcc ttcgccacat cggcatcact
     1261 gcgagaatcc ggatcattat taagacgccg cagacccgcc acatccggta cggtctcaat
     1321 atcaaaaact aagatgctgg ggatggtttt agccaaatca attactgcaa gaattggatc
     1381 gggttgacgg gtttgccatt gacgcgcact tcaaaatgta gcttcaccat attagcgtcg
     1441 gagtcgccca tctctgcaat acgttgtccc ttacgaaccg agtcaccctc tttcactaag
     1501 aggctacgat tatgggcata cgctgtgagg taggtgttgt catgcttcac aatcacgaga
     1561 ttgccataac cgcgaagact gttaccggca tagaccactt tgccatcgct agcagcctga
     1621 atcggctcgc ccaatttacc ggcaatatcg atgcctttat ttttaccctc gctgaactct
     1681 tccacgacct ttcctttggc aggccaagaa agtttaattg gaggatccac agcatcggct
     1741 tttgcttccg caacttcttt ttcttccacc ttcttcggtg ctgctttttt atcagcagcg
     1801 gccttcgcat ctttactatc ggcaggcttt aggggttcaa tgggtttggt gctcacgcgt
     1861 ggacctggcg gtggtctcac ccgcaaaagc tgatcaactt caatgagatt ggggtctgta
     1921 atattgttcc atgcggcgat gtcgcggtaa gactgcccat tatccaaagc gatccgcaag
     1981 agggtgtcac cgcgctttac tcgataaaag cctggggccg ccgcttcgag tgctggggtg
     2041 gtcgcaccga tggtgctact gcgatcggta atggtcgctt gcttggtacg tggtggagtc
     2101 gagcatgctg caaggcaaaa tgccacagcc agagcaagca atactctgag tccgtcagta
     2161 ggcaaaataa gtggggattg ggggatcgct aatttactca tatcacccct gattgtaagg
     2221 gcacaaaaaa gacctcatca agtgcggtcc tttggtaacg gtgggagctc acccgctcaa
     2281 ttaataagag ctgctgctcg gtctcgcttt tagagaccgg ggcgatcaaa cgtccgccaa
     2341 tggctaattg atcaagtaag gggtcaggca cccccaatcc agcagccgcc agaataatgg
     2401 catcaaaggg agcggcctgt ggcagaccac gaatgccatc accataaatc agtctcaggt
     2461 tggcaatccg aaaggggcgt aacttatttc gggccagatc gtgtaaagga cggatccgct
     2521 cgatcgagta cacctctttt gctaagagac ttagcactgc tgcttgataa ccacagcccg
     2581 taccgatctc caatactttg ccaaggggtt gcttgttctt gtggagtaac tcgatcatgc
     2641 gccctactac cgatggcttt gagatggttt gctgatgacc gatgggtaat gcagaatcct
     2701 cataggcttg tgcatgtaag ccagcatcca caaagggatg cctaggtact gtagcgattg
     2761 cttgcaatac attggggtgc ttaacgcccg cctcatggac ctttctggct aggtcttgcc
     2821 gatgggctgc gtagcgctcg ggagatttca tccacgatcc cagccagagg accgcatact
     2881 ggcttgccgc gcatgattgg tgagatccaa ttgcatcggt gtaatcgaga tacatccttg
     2941 tgcaatcgca tgaaaatccg ttccctcaga aaagtctcgt gcctcacctg ctggcccaat
     3001 ccaataaata tcttcgttac gtgggttctt ctgcacaatc acaggttgcg agtggtgacg
     3061 tgttcctaaa cgcgtaatac gccaacgctt gagctgttca taatttccgt tagggatatt
     3121 gacgtttaac aaggtagcaa taccatcgcc tcgctcgaga ggctctttga gtgtttgctc
     3181 aacaatatcg cgtgcagcgt tagcggcatc ctcaatttga gcccagcctc gatcgacctg
     3241 cgagaacgct acacccggta caccaaacat caccccctca atcgctgcgg cgaccgtacc
     3301 cgagtacagc acatcctctc ccatgttctc gccctgatta ataccagaga ccacaaggtc
     3361 gggcttctca tgcaaaaatc cggtcatagc catgtgcaca caatcggtgg gggtgccgtt
     3421 cacaaacaaa aagccatcac gctccccacc tgccacccga tgaatactca gaggtctaga
     3481 gagggttaac gagttggagg cgccgctatg gttttgctcg ggagagacaa ccgtaatttt
     3541 gccaaggggt cgcaaagcat tgaccaaggc caagagccct ggggcaagat agccatcatc
     3601 attgctaact aagatatggg gtgtgcggct catgattcat tccatttatg tcattgatcg
     3661 tcggtctagt aaagcgcggg ccaatgtacc agcatcgaca tactcaagct cgccaccaac
     3721 cggaatacct ctagcgatcc gtgttgcttt gatgcctttg agttttaaaa tctcgccaat
     3781 gtaatgtgcg gtggcctcac cctcactggt aaagttagta gcaagcacaa cctcacgcac
     3841 tgggccacca aactccaatt tctcaatccg atccagtaag cgatcgaaat gtatctcttt
     3901 ggggcccatc ccatctaatg gtgagatgcg acccattaat acaaagtact gtcctttaaa
     3961 gctcaaggtt tgctctacca tcacctgatc tgctggggtt tctacgacac agagtaaggc
     4021 aggatcgcgc cgtgagtctg agcaggttga gcagagcgcg tgctctgaga aggtattgca
     4081 gcgctggcaa tgcccgatct tttgaacagc atcccctaag gactctgcta atactgctgc
     4141 gccattgcga tcgtgttgca acaagtaata cgccatccgt tgtgctgatt ttgggccaac
     4201 gcctggcaat actctaaggg cctccaccaa gcgcgctaat gcatcttgcg gtacatcatg
     4261 acggttgttt gagcggctca ttcccgatct tagaaaggta gcttaaagcc aggtggcatt
     4321 ggcataccag cggttgctcc agacatcaat gcctgacttg ctgcctccgc ttgtttaaat
     4381 gcatcagcgt aagcggtcac catgagatcc tcaagcatct cacgatcatc cattgctgct
     4441 gggtctattt gcactcgcct catctcatac tttccactga tggtgatctt gaccaaacca
     4501 ttggctgcct gaccggtcac ttccatcgcg ccaagctctt cttgagcccg cttcatcttt
     4561 tcttgcatct gctgagcctg cttcatcaag cctgcaattt ggcctttcat catggtgtct
     4621 atatctccaa tggtgttctg taatgtataa aaagggtaat caattaacca tcatatgggg
     4681 cgcacggatc cagtgaccac cttagcccca aactcacgct ccaactcttg taagaatgga
     4741 tctttcgcca tttgctcttc agcattgagt tttttctgtt catagatnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcta attgcgacat ggcagtggta
     4921 atggtcacca agacggcatc gggggtatcg cgccattcct gtaactcggt ttgaaatgcc
     4981 agttgttgcg ccaatccccg cagtggcaag gttctcatca gcgcatgcca atcgggtgct
     5041 tcctgactac tcgctgtggc cttactgggt gcagcagcag ctttgggagg agttacagcc
     5101 ggcgctgcgg caggcgctgc aacaggagct gcggcggtgc ttgctggagc tttattgctg
     5161 gagaccgcag ccttgagtga agggctagta ggactaatgc cagtcgctcc actgccgcc
//
LOCUS       DDPQ01000131           38313 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_7479,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000131 DDPQ01000000
VERSION     DDPQ01000131.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 38313)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 38313)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..38313
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_7479"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2990..3139
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4488..4516
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7049..7117
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12030..12080
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18964..18986
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19206..19265
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    32619..32644
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    37989..38032
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9840 a         9089 c         9324 g         9608 t
ORIGIN      
        1 tgatggtgcg aaagcttttg atgatttaca ccaaggcaaa agcccttcag caaaaatcct
       61 tttaaaaccc cattaatatg ccatctctag atagtttttg gatgcccttc actcctaatc
      121 gggagttcaa agcatcgcct cgtatggtct cttctgcatc tggtgtgaca taccagacag
      181 aggatggaaa aacgatatta gatggaacct ctggcttgtg gtgtgttggt gcaggtcaca
      241 ggcatcccaa aatatctgaa gccatgaaga cccaaattga tgtacttgat tacgcatcta
      301 gttttcaggt aggtcatcct ggggcgttta agttaacaga ccgtattgca tcgctcgcac
      361 ccgccggtct agacagagta tttctggtga atagtggttc tgaagcatgc gataccgcca
      421 tgaaacttgc cttagcgtat tggagagtgc gtggtgaggg gcaacgtaat ttatttgtcg
      481 gccgagagcg cggttttcat ggtgttggat tcggtggaat ttcggttggt ggaatggtaa
      541 ataaccgaaa tgcctttgga acctcgctat tgcgttctga tcacctgcgt gcaacctggg
      601 atccaagtac ccaggccttc gtaaaagggc agccagaaca tggcgtagag atggccgatg
      661 agctggaaaa ccgcattatt gccttacacg gtgctgcaaa tatcgctgca gtggtcattg
      721 aacccattgc gtgctcaaca ggcgttttag tgccccccaa gggatactta aaacgaattc
      781 gtgagatctg cgatcgtcat caactcttat taatctttga tgaagtgatt acaggattcg
      841 ggcgaactgg aaatatgttc gcctcgacca cctttgatgt tgtgcccgac atgatcttgt
      901 ttgctaaaac cgtaagtaat ggagcagctc ctttgggtgg cgtcatcgta aaacgctcta
      961 ttcatgatgc attgatgcaa ggccccccgc atttatctga actaatgcat gggtatacgt
     1021 attcaggcca cccattagcc tgcgctgctg ctatagcaat gattgatgtt atcgagcagg
     1081 agaatttgat cgatcaggta aatactttgt ctccctactt tgaagaagca gtccattctt
     1141 tggctgatct tgagcaggtc gtcagcgttc gtaactttgg cttagctgca gggattgagt
     1201 taagggctaa agatggcgct gttgggcaga gaggatttca ggctcaggca ttagccttta
     1261 agcatggagt cttaacacga gcaccagggg atactttagt tctagcacct ccatttatta
     1321 caacgcgatc acagatcgat caaatgattg atgggtttaa aaaagccatt caagaaacag
     1381 tttaggtggt aaccaaccca ccatgacgca acagagcatc aatctgtggc ggtctccccc
     1441 ggaatgcttt aaaagactcc gccgcaggac ggctaccacc aacctctaaa atctctttgc
     1501 gatatcgttt gccagtttca gcatccaaga ccgtaccctt ttgcttggcc gcctcctcaa
     1561 atgctgagta gacatctgcc gataggacct cagcccattt atagctatag tagccagcag
     1621 catagccacc ggcaaagata tgactaaagg tattgggcca acgcgagata gttgcttggg
     1681 gtatcacatg gaattggtta ttaatctctc gggctagatc aaggaccgct tgcccctgcg
     1741 cagtctgagc atcgaattga gaatgcaatc tccagtccgt cagggagaac accaactgac
     1801 gcatagtggt caacccattc tgaaagttct tggcagataa catcttgtta tagagatcac
     1861 gcggtaatgg caaaccggta tcaacgtgcg cagtcatttg ttggagtacc tcccactccc
     1921 aacagaagtt ctccataaac tgacttggta actcaacagc atcccattca accccattaa
     1981 tcccagagac ccccaaagct ccaacttgcg ttaagaggtg atgtaggcca tgcccacttt
     2041 catggaacaa ggtaatgaca tcgtcatgtg taattgtggg ttggcgcagc tgaccgttga
     2101 cctcaaccgg tgctgcaaag ttacaaacaa gataggcgac aggcgcctgt atctcgccat
     2161 tggcaagaag acgacgtccc cgcgcatcat ccatccaggc gccaccccgc ttaccagagc
     2221 gcgcataggg atcaagataa aagtaggcaa tgatttgatt attagcgtta cggatctcaa
     2281 aagattgcac gctggggtgc cagattggca aggaggcagg ctgaatgttg accgagaaca
     2341 gggtctgaat aaccttaaac aggccatcta agaccttcgg caaaggaaag tattgcttga
     2401 gctcattctc agagaaggca taacgttcat gcttgagtct ctctgagata aaagccatat
     2461 cccaaggctc aattccgtcg actagaccta aagagcgagc aaattcttga agctcattcc
     2521 aatccttctg ggcaaaaggt cttgcacgtt tggcaaaatc agttaggaag ctatccactt
     2581 cactcacatc acgcgccatt tttggggcca agctaagcgc tgcgtaatta tcaaagccta
     2641 acatgcgcgc ctcttcatca cgcaaacgca gttgctcaat catattgtgg gtgttatccc
     2701 attcgatatt acccttgctg tattgcggag ctagctcaga agcacgcgtg acataggctt
     2761 catagagtaa gcgacgtaat gagcggttct gggaatattg ttgaacggga tagtacgaag
     2821 ggaaatgcaa ggtaaatgcc caaccttcta aacctttctg tttcgctgta tctgctgccg
     2881 ccgcaatggc atcctcaggt aaaccaatta aatcggcttg actactcact agatggataa
     2941 atgaatccgt tgcatctaag acatgatcag agaaggcctt ggctaaagcn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnng gcaacatcgc accataagca gcgcgtagct ctggagtgtc
     3181 agccactgca ttgagatgcg agaccacgcc ccaagagcga cccaattgtt cagtagcatc
     3241 ctctagaggc tctgctagat cattccatga agcaggcgtt ttgggatcaa tggccttggt
     3301 cactgcagcc tcgcagccac tgagtaagaa ctcaatcgca ggagcaattt gctccggctt
     3361 aacttgatcg tattgagcaa tgccacgacc aaaagagaga agggggttgc tggagggatc
     3421 tggggaggag gtattgataa gtgcgttcat gccattagct tacaagaatg tagaaaagcc
     3481 ggtttcccct acaagaagag ggcgataggc cactaaagaa aacgattact aagacagctt
     3541 actagctggc ttcatgctgc gctctaccgc ctcgagcgtg ttcatgagaa gcatggtgat
     3601 ggtcatggga cccacaccgc caggcactgg ggtgatccag cctgcaacat atttggctgt
     3661 atcaaaatca acatcaccac acagcttgcc atcggattgg cgattaatac cgacatcaat
     3721 taccactgaa ccaggcttca ccatattgcc agtaatcatc ttgggcttac cggtagcaac
     3781 taccagaaca tcagcctctt tggtatgagc tgataaatct ttggtcttac tattgcaaat
     3841 cgtcaccgta gcacctgctt gcaaaagaag catagccatt ggcttaccca cgatattgga
     3901 ggccccgata accaccgcac gggcgccgcg caaaggataa tcaatactct ccaacatctt
     3961 catacagcca taaggggtgc agggcttaaa gagtggtgca ccaaccatta aggcacctgc
     4021 attagcaata tgaaaaccat cgacatcttt atcggaggca atcgactcta aaacacgctc
     4081 ggcagagatg tgtttaggta aggggagctg taccaaaatt ccatgaatgc taggatcggc
     4141 attgagggtt gcaatgcgag caagcagttg tgcttcctca agttcggctg gatagcgctc
     4201 aaggaccgaa tgaaagtcaa catcctcaca tgccttaacc ttattacgaa catatacctg
     4261 actagcggga tcatcgccca ccacaatcac ggctagaccc ggacgcacac ctttggcggt
     4321 cagaatcgct gagcgtgtag caatctcagc ccgtaatttc ttggagagca gattgccatc
     4381 aagcagttgt gcaggcatag gggtaattaa gagagggcta aacgcaggag atcggcaacg
     4441 gtattgacgt tgagtttctc cataatattg gcgcgatggg cctcaacnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnacaa tccgctcgag cacctgacgc tcacgacccg ttaacttgcc
     4561 caataaactc tgggtgctct tacgttgatc ggcctgtgca aaatcaatcc gtgctttggt
     4621 taacatgcgc tcaaccagag cacatagctc attctctttg aagggcttct ccacaaaatc
     4681 gacggcaccc cgtttcatgg tcgagaccgc catcgagacg tcaccatgac cagtaataaa
     4741 agagatgggc atcggcaagc cttcactgat taaacgttct tgtaactcaa gacccgacat
     4801 gccgggcata cgcacatcga ggatcaaaca cgaaatagtg gacttgtcgg tattttgaag
     4861 agactgcaaa aaacgttcgg cgcttgcatg gcaacgaacg gtgtagccat tactttctaa
     4921 taaccaagtg agggagtcac ggactgcctc atcgtcgtca acaacataga caacttcagc
     4981 ttgtactgtt ttcggtggat tgcttagatt catcggtatt tcctattgac gacttcgata
     5041 tatgaccaga ttccaaaggt aacagaattg taaaggtgca gccgaccaaa cgggtatgtt
     5101 cggtatccgt atgatttttg gcccacaaac gcccgtggtg agactcaata atggaccggc
     5161 aaatattgag gcccatcccc atcccatcct ctttggtact gaagaagggc tcaaacatcc
     5221 tagagatcac ggattctgca attccagcgc ccgcatcggt gacttggata cgaagcatgc
     5281 ttgggaaggt gctggtctca agatcggcag tgattcggac gggtggcgct gaccagcgcg
     5341 aggaaagggg gtagacctct cgcaggctat ctagggagtt tttcaggaga ttgaccaaga
     5401 cctgttggat taagactgga tcgagttcaa ccgccggaag attgccggct acctcggaaa
     5461 cgatcttgag tcgatggcga ttggcctcaa attccactag accaaccgaa tcggccacga
     5521 tatcggcgat attcgaaggc ttactttgtg gctcactccg tttcacaaaa ccccggatcc
     5581 gctgaataat cgtgccggca cgatgagcct gatcgctggc tttttgcagg gcaggaagca
     5641 attccgaaga gagtggatta ttggtaccgc taagacgctt ggcaattccc atgcagtaat
     5701 tagagatcgc cgataaaggc tgattgagct cgtgtgctag tgaggacgcc atctcgccca
     5761 tggtggtgag acggctggta aattgcatcc gctcctcttg ttggcgcgct agatcctcag
     5821 tcgtttttct gagggtaata tcggtcgcaa tgagaagttg tgccaagtga ccatcaaccc
     5881 aagagatata acgtcgccgt acttcgtacc aatgggtcgt atcgccccga agctggattt
     5941 cttcggaatc aatttcagtc gtttgattaa gtgccattgc gggaagaccg gctaacgcat
     6001 ccacattgtc tccctcaaaa tgaaagattg aggagggatc catctcatga ccggctaaat
     6061 caatatgacc cttggcggta tcaccaaagc gctcgcggta atatcgattc gcaaacaaga
     6121 gctcacccgt ttcaatcgag accaccgata ctgccgcatc cagtccttct agtacggtca
     6181 cgaaccgttc ttgcgttgcg cttaattctt cccgaatacg cttaggctct gagatatcaa
     6241 tcacagaagt tacccacccc gattgttgcc ccttgtcatc aatcagaggg gcaatgaagg
     6301 tccgcgtgga gatccgcgag ccatctttac gcatgatcgt gccctcgata ccgctcttga
     6361 gattaccgtc tagggcaccg ctcatctttt gcatcagttg ctcatgaagc tcagcgggcc
     6421 agaacgggaa ggggggcgta agacccaata actcttcttg agcccaaccg gtcatctctg
     6481 agaacgaacg attgacatag gtgatgcgtt tgtctaaatc gtgtgcccga ataccaacag
     6541 gcatcgagtc ttccaagacg cgacgaaagc tactctcggt ccgcaagttt gcttcggtca
     6601 gttgccgcac ctgcatttgt ttgagaacag accatagact ccagaccacg aaggcagaca
     6661 agccaatgac cacaccaagc agcatccgaa aggtcaggtc ggtgggcggt gggtaggtgt
     6721 caacacgcaa ggtgatatta ggacctagag caccgaaatc gagactggtc tcattgctga
     6781 gagaccgggt tggggtgtcg cgatctgatg aaatcgccaa gacccgatta tcacgaccaa
     6841 tcagtgagta gcgatgctga ctctttagct ctggcggaat aatgtattgc aagatccctt
     6901 gggttgagta caacaccgct agggcaccct ttgcttctcc atccttgaag atcgtaatga
     6961 cctcccaaaa gatagactga cgttctgcca tcaccggatc atcttttgca acattaagac
     7021 taattacttg actgtaagca ggccggttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga ctgggtggtg gcaccatcca
     7141 ctggcgccat tcactgtcat tgagccaaat aagttgcaag atttcagggt tgcccaatac
     7201 aaaggattga acttggctca atatcttgtc ccgtgcagca cgattatttt cagtttgagc
     7261 caaatctcga ctgaaggatt gcaaagtctc gctattatta ataaagcgta attggatacg
     7321 ttgctttact aaactgagtt cacgaaagag ggagccttct tgctgttgac gctcctgaac
     7381 ttgaaggacg cccaaaatga tcgccatcac caccaaaaaa atcacgatcg ctaataatgg
     7441 ggtgtagacc aagcgcgaac caataaaacg acgtagcgaa ggcattcgtt tgaatgcctc
     7501 tacgagtctt aattgcaggg tgctggtctt ggacaagcta gagatccttt taaatagtct
     7561 tattttttct tgaacaccct aatataaagg gctttgcact gcaatataat tccatattgc
     7621 gaaaaagatt atcgttatgt ggaaaattaa ttgtcatatt caacacctat tcttaaaata
     7681 ttaaccataa ggcaaacaaa atttgccgta actaaaaccg gagacaaacc atggcagctg
     7741 taccagagca aatggcgggt caagtgaacc aaaatgatct tgatccaatc gaaacaaaag
     7801 aatggcttga ggccttagat ggcgtgattg cgcatgaggg aaccgatcga gccgcatttc
     7861 tgatcgacca acaaattgcg catgcacggg tgaatggagt ggatcaacca tttcatgcag
     7921 agaccccata catcaatacg atacccgtag agatccaagc acgtattcct ggtgatcaaa
     7981 acattgagca tcgcattcgt tcatacacgc gttggaacgc gatggcgatg gtgttacgtg
     8041 ctaataaaga tacgaacgta ggcggacaca tttcttcgtt ccaatcggca gccaccttgt
     8101 atgacgttgg ctacaaccat ttctggcacg caccttctga acagcatggt ggtgacctaa
     8161 tatttgtgca aggccactca gcccccggtg tgtatgcccg tgcttatatg ctgggacgtc
     8221 ttagcgatga gcaactcaat aatttccgtc aagaagttaa tggcaagggg atctctagct
     8281 atccacatcc ttggctcatg cctgacttct ggcagttccc aaccgtatcg atgggtcttg
     8341 gtcccatcat ggctatttat caagcgcgtt tcatgcatta cttgcaagat cgaggtcttg
     8401 caacgacggc agggcgcaaa gtgtgggcct tcttaggcga tggtgagacc gatgaacccg
     8461 agtccttggg tgcaatcggt atggccgggc gcgagaaact cgataacctc atctttgtag
     8521 tgaactgcaa cttgcaacgt ttggatggtc cagtacgcgg caatggcaag attattcagg
     8581 agctcgagag cgagttccgt ggttctggct ggaatgtcat taaggtggtg tggggcggtc
     8641 attgggatgc actctttgcc cgcgacaaaa aaggcatctt gatgcagcgc ttagctgaga
     8701 tcgtggatgg acagtatcaa acgatgaagg cgaagaatgg tgcctatgtg cgggagaccg
     8761 tatttaatac acctgagctc aaagccctcg tggccgattg gagtgatgag gacatttgga
     8821 acctaaaccg tggcggacat gacccccata aggtctacgc tgcattccac tctgccgcta
     8881 atcacaaagg tcaaccgacc gttattttgg caaagaccat caaagggtat ggcatgggag
     8941 gctcaggtga gggcatgaac attgctcacc aagctaaaaa aatgagccac gatgatgtgg
     9001 tgcgcttccg ggatcgtttt gagattccgg tgagcgatga tcagttggaa gagatgcctt
     9061 tagtaaagtt ccctgaaggt agtcctgaac tcgagtacat gcggacccgc cgcatggagc
     9121 tcggtggtta tttgccacag cgtagaacga aagcggagag cttgccggtc cccgcactcg
     9181 atgcgttcgg accattgctc gaagccacta ccgatgggcg cgagatctcc accaccatgg
     9241 catttgtgcg tctacttaat accgttgtgc gcgataagac catcggtaag cgcgtggtcc
     9301 ccattgttcc tgacgagtct cgtacctttg gtatggaagg gatgttcaga cagttaggga
     9361 tctggagcca actcggtcag ctctatacgc ctgaagatca tgatcagctt atgttctaca
     9421 aagaagataa agcaggtcag atcttgcaag agggtatcaa cgaagcaggc ggtatgtgcg
     9481 actggattgc cgcagcgaca tcgtactcga cacatggtgt acccatgttg cccttctata
     9541 ttttctattc catgtttgga ttccaacgta ttggtgatct tgcttgggct gcaggcgata
     9601 tgcgtagccg cgggttcttg ttgggaggca ctgcaggtcg aaccacactc aatggtgaag
     9661 gcttacaaca cgaagatgga catagtcaga tttgggcatc ggcggtacct aactgcatta
     9721 gctatgaccc cacctttgcc tttgagttag cggtcgtgat ccaagatgga atgcgacgca
     9781 tgctcaccga acaagaggat gtgtattact acgtcacgtt aatgaacgag aactattcac
     9841 accccgcgat gcctaagggt gcagagcaag acatcatcaa aggaatgtat cgcttgcaat
     9901 ccgtgggtga ggcaaataac ccactacgag ttcaactgct tggtagcggt acgatcttcc
     9961 gcgaagtgat ggctgctgca gagatgctgc acgccgagtg ggggattgca tccgacctgt
    10021 ggggttgccc cagcatgaac gagttaggac gcgattggaa cgagaccaac cggagtaatt
    10081 tgctgaaccc aggtaacacc ccaaaactct cgcatgtaga gcgcctctta aaagacacca
    10141 aaggtcctgt gattgctgca acggactata tgcggatgtt tgctgaacaa atccggccac
    10201 cgattcaaaa tctaggacgt cgttatgagg tcttgggtac cgatggcttt gggcgctcag
    10261 atacccgcga gcaattacgt cacttctttg aagtagatcg tcgctggatc actgtagctg
    10321 ccctgaaggc gctggcagac gatggcaaga tcgatcgcaa gaaagtagct gaggcaattg
    10381 cgaagtatca gattgatccc aaaaaaccaa atccaatgaa ggtgtaaggg aatcatgagc
    10441 cagctaattg aaatcaaggt cccagatatt ggtgactaca aagacgtgcc cgtgattgag
    10501 gtacacatca aaccaggtga caccattgag aaagagcagt cgctcgtcac actagagtct
    10561 gataaagcaa ccatggatgt gccatcctca cactcgggag tggtgaaaga agtgaaggtg
    10621 aaggttggtg actcgatctc agaaggaagt gttgttctgc ttctagaggg ttctgaggcg
    10681 cctattaata cagctacgaa tacacctgca ccaacgcagg ccccagcacc tgtaaaaact
    10741 caagcacctg cgcctacacc tgcacctgca ccaataccag tagcgccaac accgatcgct
    10801 gcgccagtat caactcaagc acccgcccct gcacccgtgt ctactccatc agcagagcta
    10861 ggtcaaagcg gtagtcatgc aagcccatcg gtacgcaagt tcgctcgtga gttaggcgtc
    10921 accattgcta gtgttaaagg aacgggacca aaaggccgta ttacgcaaga cgatgtccag
    10981 gcttttgtta agtcggtaat gagtggcgct agtgcaccgg ctgcaactag cggtggaagt
    11041 ttgggtggac taaacctctt gccatggcca aaggtggact tcagtaaatt tggggagacc
    11101 gagcgtcaag cactctcccg catcaaaaaa ctatctgccg ccaatcttgc tcgtaattgg
    11161 gtgatgatcc cagcagtgac ctatcacgaa gatgcagaca ttactgatct ggaagcattc
    11221 cgtgtgcaaa ccaataaaga gaatgagaag caaggtagca agatcaccat gctggcattc
    11281 ttgatcaaag cgtctgtcgc ggcacttaag aagttcccag agttcaattc ctctttagat
    11341 ggagatgatc tcgtactgaa aaagtatttc catattgcat ttgcagcaga tacccccaac
    11401 gggttggtag ttcctgtaat tcggaacgcg gatcaaaagg gtatctttga tatcgcccgc
    11461 gagaccgccg agcttgctgc attagcgcgc gatggcaaac tcaagcccga tcaaatgcaa
    11521 ggtgcgagct ttacgatctc ttccttaggc ggtattggcg gcacctattt tgcccccatc
    11581 attaatgccc ccgaggtcgc aatccttggt gttagtaaag cagctatgaa gccggtgtgg
    11641 aacggcaaag agtttgcacc ccgacttatt tgcccactct ctttgacagc agatcatcga
    11701 gtgattgatg gagcattggc tacacgtttt aatgtctata ttgcacaact actcgccgac
    11761 ttccgtcgtg cagttctata ggggatcgcg atgagtcagc atgcaatcgt ggtgcctgat
    11821 attggtgact acaccgatgt cccagtgatt gagatcttgg tcaagcctgg tgatgagatt
    11881 acaaaagaac aagccttggt tgttcttgag tctgataaag caactatgga agtgccagcc
    11941 gatcaatctg gcaaggtcat tagtgtaagc gtgaagattg gggataaggt tgctaaagga
    12001 tctgtgattg cgcagattga actcgctagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn catcaagcaa gccacctgta tcgatcccag tacctgctgg
    12121 ttcttatcaa ggaccggttc aacacgagtg tgaggtcttg gtcttaggag cgggacccgg
    12181 tggttacagt gccgcatttc ggagcgcaga ccttggagct aaaacaatat tagtagagcg
    12241 ctatgccacc cttgggggcg tttgcctcaa tgtaggttgc attccatcta aagcattgct
    12301 gcataccgcc gcggtgatgg aggaggtgaa ggagatgggt aagcacggta tcgagtttgg
    12361 tgcacccaaa attaatttag acctattgcg ttctcataaa gagggagtga tcgcaaaatt
    12421 aacgggcggt cttgctggta tggcaaagat gcgcaaggta ggggttgtgc gcggtctagg
    12481 tagattgatc gacgctcatc atgtggaagt gtctttaagt tctggtgatg gtcagcaact
    12541 cacaggtcag aaagaaatca tccgttttca gaaagcgatc attgcggcag gcagtcaacc
    12601 aattgctctg ccgtttatgc caaatgaccc tcgggtagtg gatagtaccg gcgcattact
    12661 gctcaagagt attccgaagc ggatgttagt gatcggtggc gggattattg gtctagagat
    12721 ggcaacggtt tatagcgctc tcggctcgcg cattgatatt gctgagatga tggatggctt
    12781 aatggcaggt gcagatcgtg acctcgaaaa agtgtgggag aaaatgaacg cccatcgctt
    12841 tgacaacatc atgctcaaaa cgcgagcaag caaagccgaa gcaaaaccca atgggatcga
    12901 agtgagcttt gagggtgaga aagcaccagc cacaccacaa ctctatgatc ttgtcttagt
    12961 ggctgttggt agaaccccca atggtaagaa gattaacgca aatgccgccg gtgtcagtgt
    13021 ggatgagcgt ggctttattc cggtcgatca gcaaatgcgc accaatatca accacatctt
    13081 cgctattgga gatattgtgg gtcaacctat gttggctcac aaggcggtac atgaaggtca
    13141 tgttgcagcc gaggcagcag ccggacaaaa gtcttacttt gatgcaaaac aaatcccgtc
    13201 ggttgcgtat accgatcccg aggttgcttg ggctggtttg accgaagagc aatgtaaggc
    13261 acaaggcata gcttatgaga agggcttgtt tccatgggca gcgagtggtc gtgccatagc
    13321 gaatggtcgc gacgaaggat ttactaagtt actctttgat gccagcaccc atcgcattat
    13381 tggtggcggt attgtgggca ctaatgccgg cgatctgatt ggtgaggtct gtcttgctat
    13441 tgagatgggc gcagactcgg ttgatattgg gaagaccatt catccgcatc caactctagg
    13501 cgagtcggtg ggcttggcag ccgaagcgca ccatggtagc tgtacggatt taccaccttc
    13561 taagaaacgc taggcaataa aaaaccccgc aatgcaaatt gcggggtttg tcgtctaaag
    13621 aactaattta gttcttaacg caacattact tcttcgaagc ctttgatgcc ttcgtagcag
    13681 ctttggtaac tgtatcaaca gcgttgtgta cattgctttg ggcaacttct acagcatgct
    13741 taattgcctt ctggctggtt tcgtagctat tgtttgctgc agcaatggct tgtttcatca
    13801 tttgaacagc tgcatcagaa ccggctggtg catttttggt ccagtcatca atcatcgcgt
    13861 ggagcttctt ctgacctgct gccatttcag cttcagcagt cttagcaaat gcagccgtat
    13921 tgtcttgggc gatctcatac agatcacgtc cataggccat catcttctca gccatgggtt
    13981 gaaccatctc tgcttgttgt gcaatcaatt gctgcagatc acgaacttca agggtcttct
    14041 tggcattgct catgctctca ctgaggctgt tcttagtaat ttgcatattg agttcgacta
    14101 atttctcaac gcttttgatt gcttggttgg ttaaacctgc caaggtctct aaattagctt
    14161 tttgtacagc agcgatttgc tctggggtta agttcatggt gttctccgat gagtgataag
    14221 ggtagtaatc ctgatcttta gaatcagctt ataggaatta tatcgataac tatgttgcaa
    14281 tgcaacaaat actattaacc ctaaagctaa tataaataac ctaatataat caatcactta
    14341 aatatcataa atcaataaaa tccgattgaa agccttctac agcgaccact ttgtattgcc
    14401 attacccgag ggtcatcgat tcccgatggc gaagtacaaa atgttgcgcg actcggtaga
    14461 gggcttaccc aacctagaat tggtagaggc tccctgcgcc acggattctg agctcttatt
    14521 ggcgcacgac cccagttata tacaagatgt gctccatggc accttgagtg aggctcagca
    14581 aaaagagatt ggttttcctt ggagccctaa gatggttgag cgctcgcgtc gctcggctgg
    14641 ggcaacgatt gcggcgtgta aagcagcact tgaagagggc atctctgcca atttggccgg
    14701 cggcacgcat catgcatatc gcaataaagg ctcaggcttc tgtgtattca atgacgcagc
    14761 cattgcagcc cgagttcttt taaaagactc acgggctaaa gcagggggca tacgtaagat
    14821 tgggatctta gatcttgatg tgcaccaggg cgatggtact gctgccattt tgcagcatga
    14881 ttctgcagtg attacggtct ctgttcatgg agagaagaac tacccctttg caaaagaagt
    14941 aagtgatctt gatattgctc tagctgatgg cagtacagac tcggtttata tgcaggcgct
    15001 tgaacaagct ttgatctact taagtgagca aaaaattgat ttcttaattt atctagcggg
    15061 agccgatccc tattacgaag atcgcttagg tcgtttacaa attagccaag agggtatggc
    15121 actacgcgat tacaaagtat tggagtttgc tagcacgcaa ggaattgcaa tggcaatggc
    15181 tatggcagga gggtatgcca atccgattga gaaaaccgtt cagatccatg cgcagacaat
    15241 tgggctcgcg cttgcgcatc agcatacaat tgcgacatct ttattgaagc gataagtaat
    15301 gagtctagcg tcggagcgtt tcccccttga atccctagcg gcgcccatct ttgtttttat
    15361 ctggagcacg ggctttatca tcgctcgctt tggtatgccc tatatggagc ctgccacttt
    15421 cttgttcttg cgctttaccg gtgtactcat tgtgatgggc atcatcatcc tgttctggaa
    15481 acccatttgg ccaaatcgtt cacaaacgat gcatattgct attgccggtg cgcttctaca
    15541 gtttggctat gtgatgggtg tgtggagcgc ggtgcgtttg ggaatgaccg ccggtttggt
    15601 tgcattgatt gtgggtctac agcccatcct aaccgcttgg agcgcctcat gggtagcaga
    15661 gcgggtgagc tatcaacagt ggtttggact actactcgga attgctggtg ttggtctcgt
    15721 cgtggccgag aaaacaagtt ttatgaacat ccccttggct gcttatatct ttgcactcgc
    15781 tgccttgttc tcgattacct ttggcactct ctatcaaaaa aaatactgcc cccaatttga
    15841 tttgcgagca ggatctatga tccagttctc aatatcaacg ctgttatgcc tgctggttgc
    15901 acttctcttt gagacccgcg agattgtctg gaatgcgccg gtgattggtg ccttgctttg
    15961 gggcatctta ccaacctcga tcggtgccat tagtttgtta tttattctga ttcggaaggg
    16021 cgccgctact aaagtaacta gtctgcttta tctgacacca cccactacgg cactaatggc
    16081 ctggctcttg ttcgatgagc ctttgagtct catgatgctt gccggtctag gcttaaccat
    16141 gaccgccgtg atcttggtca actaccaaca aaatagttca cccaaaaccc aataacttta
    16201 ttatgatgtg tcagtactca aattcgggac tttgattgag agctttgcgt gtgttgctta
    16261 agccagtagt gccaaattta tctgattggc agagaaggac ccaactcccc ctccgtattt
    16321 tgatcttggc tgcattgtct attactctgt gtgccattga gccagcctca gcgcaatctt
    16381 caccaccccc caaaaacacg caggccaaga aacaaaagtc ggccaatccg aagcaggttc
    16441 gtaccaccgt taatcgacct aaaggaacga gcaaaaaaga ggttccagat cgcccatcct
    16501 ttgccaacgc aatgggtttg cgtaatcaac aggatgagct cagtctgaag tcaagtgtgg
    16561 cgatggtgat tgatcaacag accaaagagg ttttgtttga gaagaacccc gatgtgagtt
    16621 tgccgattgc ctcgatcact aaattaatga ccgccatggt ggtgcttgac tctaatgcac
    16681 cgctcgatga ggtcttaacg atcacccagg ccgaggtcaa aatttatgcg aagtcgcgtt
    16741 tatctctcgg caccaaacta actcgagagg aagcgctctt attagctctt atgtcgtctg
    16801 agaatcgtgc ttcacagcta cttgctacta attatccagg cggcttatcc gcattcatcg
    16861 aggccatgaa ccacaaggca tatgccttag gaatgaccca atcgagcttt acggacccaa
    16921 cgggattatt ggcaagcaat gtgtctaccg gcgaggactt ggcccgtctg ctaatagctt
    16981 cctataacta caaattaatc cgcgagttct cagtatggcc agacatgacc atggtgatta
    17041 ataaaagacc gcaggtgttc ttaaacacca accgcttggt gcgtgctggc gatatggaaa
    17101 ttggtttaca gaaaaccggc tttatcagtg ctgccgggcg ttgtttagtg atgcaggcca
    17161 aggttaatgg aatgccatta ctcctcgtgt tcttagactc cgtgggaacg caatcgcgtt
    17221 ttgcagacgc agtgcgcgtt aaggattgga ttgagagcta tcagcctggt gagcctaagc
    17281 ccattcgtcg tctgacgatg taactctatt ttttgggaat agccagcgga gctttgtagc
    17341 ccagaccttt ggagatttgc aaagcagtat cttgtaaagc cttgagccaa tctgcctgca
    17401 tccgatctgt gggtgagctt aaggagaggc ccgcaactaa tttgccagag tcatccaaaa
    17461 tacccgctgc tagacaacac actcctagct caagttcttc gttatcacgc gaatgcccaa
    17521 tatggcgcac gctagataac tcactctcca gctttgcaat ttgcgtgatg ctattgcgcg
    17581 tatgacccgc aagacccgta cgagtagcgt atgcgcggac ctggttgggg tcatcagccg
    17641 ctaaaaataa tttaccaacc gaggtgaggt gcaacggtgc gcgaccacca attgcacgaa
    17701 ctacctgcat cccagagcgt tcgctatagg cacgatcaac atagacgatt tcatcgccct
    17761 gacgaacgga gaggttaacg gtttctccag tgagcttatg caagacccgc atgggcaatt
    17821 gagcagcctc acgcacggat aagcgctcct tgaccaaatt acctagctct agaagacgta
    17881 agcctaattt ataggtacca ccatcgccac gctcaacgag ccggcacgcg accatatcat
    17941 taaggatgcg gtgggcagta gaggggtgca aaccagtttg ctgtgacagt aatttaagac
    18001 tactggactc ttcatgatcg gccaagcaat cgagtaagtt catcatgcgc tcaaccacct
    18061 gaatggcggt cttgccagct tccccagagt tcttatttgc tttgctatcc atgctttaga
    18121 ttgtagcgaa tatcgccttt attgcatatt gtgaaacgat atattaatcc aaaaataggc
    18181 gccttagcca gtgcaatcaa aaaacgggga gactaattaa aggcgcgaac gcaatcccta
    18241 attaattgtg gacctttata aatgagcccg gtatagagtt gcaccaaact ggcgcctgca
    18301 tcaatcttct gccggacatc attgcctgat tgaatgccac cgaccccaat aatgggaacc
    18361 cgattttgta atgactgaaa aaggtgatgg atggcctgat tggataagtt caatactggc
    18421 gagccagaga ggccgcccgc ctcactgcca tggggaagag tagcaacatc aacccgacta
    18481 atggtcgtat tggttgcaat cacggcatca atttcaaact ctagcaagag atcggcaatg
    18541 aatttgaaat cattagcctg cagatcagga gcgattttta agaagagtgg tttacgaacc
    18601 ccatattgat cggacagtgc gatacgtgcc tgatgtaaag cagacagaag ctgtcgcaat
    18661 agagtctcac cctgaagatc gcgtaggttt ttggtattag gggatgagat attggccgtg
    18721 atgtaactgg caactggata gactgctttc atgcacatga catagtcttg attggcatcc
    18781 tcgagagttg tgctggcgtt cttgccaata tttaaaccaa tcacaccacc ttcttgataa
    18841 aagcgtgaac gcctcacacg ttctacacac gcatcaactc catcattatt aaagcccatg
    18901 cgattaatga gagcatttgc ctcaggcaaa cgaaacatgc gaggttttgg gttgccggac
    18961 tgcnnnnnnn nnnnnnnnnn nnnnnntctt tatcaagacc agcagcaagg ccaacaggat
    19021 tacgaaatgc aataccgcat acatgaacag gcttaattag tggctcacct ataaaacgac
    19081 ttaaaagacc gcaacgttct agacgatcga gttgggcgaa ggagaaatca tgggccgcct
    19141 caggatccat cgagaacagc agcgggcgaa gtagggggta agtattaatc atgctctagg
    19201 tttggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19261 nnnnncctag ttgctgaact tgattgatct gccacaagat gctgtaagtg ccgtaactag
    19321 agtttggcat gctggcatcg taaaaggtat ataccgagga gataccttgc tcgaccacgt
    19381 cgatcatgct gaccatgcga agacgtcctg ggtggggatc gtctggccca tcccggaact
    19441 ccacaatgcg tgagttcact cggctttgta ataaaaactg catgtactgt tcttgattat
    19501 cgttctccat ttggctgcca tgatgccgct gcgcttgata gtcttggtaa agacgatagt
    19561 gctcttcatg gtgtgccaaa ctcagaactt gagcttgtaa acccgaatga cgtttccaac
    19621 tgcgtcgctg actccgattg ggctcaaagt gttgcaccgg aatgcgcgta gcaatgcatg
    19681 ctttgcaatc gtcacaatac ggtcgatagg taaataagcc actccgacga aatccagcgt
    19741 tcactaaatt actgtagaca tcctcaccaa tcgcatgtga aggggtggct acctgcgagc
    19801 gcgcaatttg ttcgggtaaa taactgcagg gatagggcgc cgtagcataa aactgtatgg
    19861 ctgtgatggg gagttcttga atacgactca taccaggtat ttctggagaa cggtcttatc
    19921 aaaatcccaa gcacatggat ctttctgagc aacagcgtgg tgcagatgct ctaaaaaagc
    19981 agtccgggtt atcggtttag caccgaggga acggagatgg tcggtttctt gttggcaatc
    20041 gattagggtc acaccatgat cgtagcacca tgcacataag gctgctaaag cgagcttgga
    20101 actatcttta acccgcgaga acatggattc tccaaagacc atcttaccaa gacaaacgca
    20161 atacaagcca ccaatcgttt gcgagtgact cacaatactg atgcaatgcg cataaccttg
    20221 ctcatgtaga gcggtgtacg catcaataat ctcgtgggtg atccaagtac catcttgacc
    20281 cgcgcgttcg gtggtcgcac aggctcgcat caagccatta aaatcatgat ccacacgcaa
    20341 ttgcatttgg ggatcgtcta aaaactgctt gatggattta ttgagcgaat gactaatctt
    20401 aagatcagct ggatttaagg tcatccgggg gtcgggcgac caccacaaga ccggttgacg
    20461 gacagagtac caaggaaaga tcccagagcg atatgcgcgc tcaagctgac ctgcaaagat
    20521 ccgttcactt accgcaagca gacccggaac ctctggatcg ggatcgctct gtgtctgtgg
    20581 atccggaaaa ggatcgcttg gtccaagcca tggaatcgtg ctcatgaaga gactatcgcc
    20641 atcatccgat tcgttcgccc ggcagaatat cgcgagagcg aaccccaaga tcgcctaaga
    20701 gcccagcacg tgcatcagca aaataccatt ggagggtttg ctttactgtc gggaatgcta
    20761 attcattcca aggaatggtc tcctctgtga agagagcaac ctctaagctt tcggtgcccg
    20821 ctgcaaattg gggtgaacgc atctgcgcta aataaaagag atgtacctgt tctacgtgag
    20881 cgacatttaa cagcgagaac aaggactgaa tctcaacgtc ggcgccagct tcttccatgg
    20941 tctcacggat tgcaccgtgc tcggtactct ccccaatctc taaaaagcct gctggtaagg
    21001 tccaatagcc atgtcttggc tcaatggcgc gccgacagag taagacctga ttctcccaaa
    21061 tggggatagt gcccaccaca ttacgtggat tgcgatagtg aacttccttg cattgcgaac
    21121 acacatgccg taaacgcgtg tcatcagccg gcactaagag tgcgatgctc gaaccgcaag
    21181 aggggcagta gttcataaat gggctttgat cattccgcgt aaggcattac tcagcattat
    21241 ttcattggcg ctgatcacat cctcaatagt caggttagcc tcacgggctt tgagggatgg
    21301 atcgtgcaag attgctgcgc gcatcactcc aggaagcacg cctgctgata gagggggtgt
    21361 taaccattca tccgagtttg gtggacgtac aaaaatactg gtgcgacccc cttcggtcaa
    21421 ctcgccacgc tcattcataa acaaggcatc aaagccaccc aaagacaccg ctttttgcca
    21481 agccaagtca tacaccttgc gttgcgtagt tttgtggagc aataaagggt tggtagattg
    21541 cattttgaga gagtcaggca caccctccaa aatctgatga gcccaaaata cttgcaccga
    21601 tgatggtagg tcatccaaga cagcgctgga gatggatagc tcacctaagc gaccaagatc
    21661 taaacgaaca cggtagaccg tatttacatc caaagccaaa gcagtttgct ctaacaaggc
    21721 acacgcctcg ttcatattca gagggatgcc caataccttg gctgaatggg agagacgggc
    21781 caaatgctca cgataatgag cgacacgccc tataccttcc tgagcattaa aacgaatggt
    21841 ctcaaagaga ccaactccac tgggtaaatc ggataagaaa gcagccttaa tcttgcattc
    21901 ttgatattcg gcacgaggat ccgagtcaat cgtaatacca gcacctacac ccaaaacaaa
    21961 ttgcgatgca aacgaatgcg agtcatgctc catttgcaag gtgcggatgg gaacgctcaa
    22021 agcaaagtcg ccattgggat caaaccagcc cagcgctcca caataccaag cgcgcggagt
    22081 ctcttctaga tcctgaataa tctccatgct tcgttttttg ggagccccgg ttactgaacc
    22141 gcaggggaac accgcttgca aaatctctgc catgctcaga tggggacgtg ccttggcctg
    22201 aactgtagag gtcatctgta aaacattgcc atggcgattg acctcaaaaa gagcaggcac
    22261 tacaacacta cccggctctg cgatacgccc cagatcattg cgtaagagat ccacaatcat
    22321 cacgttctca gcctgattct tgggatcatc cgatagctca tgcgctgcat gttggcgggc
    22381 atcggcagtg cctttcatgg gttgcgctac aaatacacca ttcgtgcgcc taataaagag
    22441 ctcgggggat tgcgataaca cgatatcgcg accatcgtcg agataagcac cataacgacc
    22501 aggttgacgc tcacgcaatt gggcataaag cgccagtggc gagccatagg cttctccagt
    22561 aatgcggtat gagtgattaa tttgataggt atcaccgcta cggatccatt cctgaatgcg
    22621 ctcaatatct tgattgaaat gctccgacgt aatcgaatct cgcaggttca tcaccccaac
    22681 caacggatct tctttcgtta aggcatcttt taaccaagca tcaacttcct ctttggagta
    22741 tttttgtact cccgtaaacg accatgcacg aacgagcggt aggggctcac tgtatttgca
    22801 tgcaatgcgc tgaaagtagt accccaactc ataagagaac agagttacga ggtaatcccc
    22861 gttgctgaga gctgtttgca tttctgcaaa acagttctct agctcgcttg tatgatctac
    22921 gcgccaagcg tgtttgacgt gttgatagag ccgactagtc gcttgctgtc ggctactctc
    22981 cacatcgtct agaagaacca tctggattat tttaggatgg tggccgattc aatcacaaca
    23041 ggagtattgg gaacatcggc catgcgacca cgtggtgtag acgcaatacc tgtaggcagc
    23101 cctttaatct tatcaatcgt ttgctgccca gaaataaccc gaccaaaaac ggtgtaacca
    23161 tttcccattg cattggggta atccaagccc ggattgtctt tgacattaat aaagaactgc
    23221 gcagtcgctg agtctggatc ggaggttctg gccatcgcaa tcgtatagtt ctgattcttt
    23281 aaaccattct gcgcctcaga gaccacaggg gcttgcgtgg gcttttgcac taaatcagtg
    23341 gtgaacccgc caccttgcac catgaaccca ttaatcacgc gatggaatac cgtcccattg
    23401 taaaaaccac ttttgacata agctaaaaag ttattgacgg tcttcggtgc acgatctgac
    23461 tcgagatcca ccacaaaatt accttgattg gtcttgaact ccaccttagg acccgcctga
    23521 gcgattgaag agagggtcac gatgctgagt gctagaccca ggctcatcca tagtttgcgc
    23581 atattgcttt ccttctgagt aggttgctga tgattgattg tattagcgat tggcctgtgc
    23641 ctgcgccagt tcattccaag cagtcgtgta gttagggcta cgctgcaaag agcgcatggt
    23701 ccagcgtggt cgcaggccgg ccccggcgag cctaagggta ttggagccat aggtgtgatt
    23761 aagacgatcc atgatttgca tcacagagcg cgagcgggca ctttctgggc tggcacaaaa
    23821 taaatcggct tgggcttggt ctgcggggta gagccctagt agcatcaccc ccgccttttt
    23881 atacgcatga ccagagcgat agatttgttg cacccctagg cttgcaagct cggccagatg
    23941 acgtgtgtca tccgttcctg ttattaaggg aatggtgacg ctattggcgt attgctcaga
    24001 ctgctgtgca aatggattgg tgcggatgta gaccaagaga tgcgagcaga aggatttctg
    24061 gtgacgtaat ttctcggcag cacggctgac gtaactcact accgcctctt gtaattcggg
    24121 aaggtgatgc acaggcttac caaagctctt gctagagata atttgatgct tacgtagcaa
    24181 atgctcgtct tgagattctt ctaaatcaag acagctaatg ccattgagtt cattgattgt
    24241 acgttctaag accactccaa agcgtttacg aatcaggcta gtggaggcgt atttcagatc
    24301 acagaccgag cggatgccaa gttgattgag cttaattgtg agacgtcgcc caacccccca
    24361 aacttcacca acctcaatgc tgctcatgag ggcatcttct tctaagcgat tgcaatgccc
    24421 ccaactaaat acacctttgt aatcaggacg cttcttggca atatggttag cgagctttgc
    24481 taaggttttg ctatgaccaa taccaatgca ggtcggtaaa cccaagtaat gcttaatacg
    24541 cttgcgcatc gcttgtgcct gagcaattag atcgaggcgc ataccactca aatctaagaa
    24601 gcattcatcg atggaataaa tttcttgatg gggtgagagc tcaccaagca gtgccatgag
    24661 gcgggcgctc atatcgccat acagggtgta atttgagctt aaccaaatta agccgtgcga
    24721 ttgcacaaag tgcttggcct caaagaaggg tgcccccatc cgaatcccca aggcgcgcgc
    24781 ttcttgacta cgcgccacaa tacagccgtc gttattggat aaaaccacca caggcttacc
    24841 ctcaagacgc ggattaaata cccgttcgca agagacataa aaattattgc catcgaccaa
    24901 tgcaaacacg gtacgcatga tcacacttta tgaacaacgt gggcaaccac accccaaatc
    24961 tgtaactctt gcccattctg gatcgcaata tcagggtagt caggattgtc ggcacgtaag
    25021 atcaccttgc cccgttgatg atgtaagcgt ttgaccgtga gctcaccatc gagcacggca
    25081 atcacgacac gaccatgatt tgcttcgagg gagcgatcga ccactaataa atcgccatca
    25141 tggatcccgg ccccaatcat ggagttgccc tttacacgca agaagaaggt tgcctctgga
    25201 tgtaagacca gatgctcatt gagatctagg cgtttgtcgt aatgatcggc agcaggtgaa
    25261 ggaaagccgg cgggggctcg accagcaatc aagggcaggc gacacagccg attgacgatc
    25321 ggcatcagga cattgggaac gctagggtca aaagggatag ttttcatata ctgtatattt
    25381 atacagtata tagtaatttt gttgcttcta aaggcccaaa gctagtcctt aagaccttta
    25441 gccttggcgt cgagctcttt taaaaagcgc atacgctctg caatcttgct ttcaagaccg
    25501 tgatcaaccg gttcatacca gtgaggttcc ttcatgccct cgggtaagta agattcacca
    25561 gcggcatacg catgtggctc atcgtgcgca tagcgataag catgaccatg acccaactct
    25621 ttcatgagtt gcgtaggcgc attacgtaaa tggatgggga catcacgact gcgatcctgc
    25681 gccacaaaat cacgcgccgc attaaatgcc ttgtaactcg cattactctt tggggcgatt
    25741 gctaagtaca ccactgcttg tcctaaagct agctcaccct caggcgagcc taagcgttca
    25801 taggtctgtg ctgcatcgtt ggccagttgc attgctcgtg gatcggctaa accaatatct
    25861 tcccaagcca ttcgcacaat ccgcctagcg agatagcgtg ggtcagcgcc gccatcgagc
    25921 atgcggcaca accaatacag agcagcatct ggatgagaac cgcgtactga tttatgcaag
    25981 gcagagatct gatcgtaaaa ctgatcgccg cctttatcaa aacgtcgtgc gttcagagtc
    26041 agggtattgg caataaagcg cgtatcaatt tcagtaacag gattatttgg aggactcaaa
    26101 gcaccttgta gttgctcaag caaattaatt aaacgacgcg catctccatc ggcataagca
    26161 acaatggtct caagtgcgtc ggcagtgaat gctgtattgg gtaaagcata tttgcgtgca
    26221 cgctctaaga gttgggcgag ctcattgtga tcaagtgact gtaagacata gacctgagcg
    26281 cgtgagagca gggcggagtt cacctcaaag gacgggttct cagtcgttgc cccaatcaag
    26341 gtaaagagac cggattcgag atggggtaag agcgcatctt gttggctttt attaaagcga
    26401 tgaatctcat ccacaaacaa aatagtttgc ttgccagatt gcgccatatc atggcgcgct
    26461 tgctcaatcg cctcacggat ttctttgaca cccgccaaga ccgctgagat cgcaatgaat
    26521 gagcgtgcaa aggcttgcgc cgataacctc gcaatcgttg ttttgccaac cccaggaggc
    26581 ccccacaaga tcatggagtg gggttgaccc gattgaaagg caagatagag gggtttacca
    26641 acaccgagta aatgggtctg accaatcaac tcatcaatct tctgcggacg caatatctcc
    26701 gcaagaggtg gtattggctt ggaatcaaat aaacccggca tgatcaaagt attctcgcac
    26761 ttttatacag aagactcatt tgtcatcaag gctacataaa tttggctaca atttgatcta
    26821 tgaatgaaaa caaaccgttt gagattgaac aaaaattacg ccccttgcca cgctggattt
    26881 ttatgtcccg ctggttgcaa gcccctttat atatcggcct aatcgttgcc caaggtgttt
    26941 atgtctggca gttctggatg gagctcgtcc acctcatcag catgatgaag gataaggaca
    27001 tgaccgagac cgctctgatg ctggtcgtgt tggggctaat tgatgtggtc atgatctcga
    27061 acttattggt catggtgatt gtgggtggtt gggagacctt tgtgtctcga cttgagttac
    27121 accaccatcc cgaccaaccc gagtggctat cgcatgtgaa cgcgggagtg cttaaggtca
    27181 aattagcaac ggccattatc ggtatctcct cgattcattt gctaaagacc tttatcaatg
    27241 cggcctcgta tgatgagaag actttactct ggcaaacctt aattcatgtg acctttgtga
    27301 tctctgcggt tgccattgcg tataccgaga agattattgc ctcagcgcac aagaaacatt
    27361 aagccagtta gtagttcccc aatagggtaa ccacacccgc ccacgataga ctgatcgatg
    27421 ccaccaccgt aaggtgggtg cgcatcctga ggaaagcatt cgcagtctcc tcattggcaa
    27481 agtaattctg ataggtgttt tgatcaatca ttcgctgcac aatcaaggta attgctaaga
    27541 gcagtagtgc gagtggaatg tagatatgga ttgccaacca tgagaccaag gctgggatga
    27601 ttccccagat ccaaacacta tgatcgagcc agcgattgtg tgagttctga ccaatggttc
    27661 taaagcatgc cccccaatgc aaagcaccca agaaggacgc aatcactgcg ccataggcca
    27721 ctaaagactc cagcgcaatt aaatcattgg gactatctgc aaactgaaca ctcaaggcaa
    27781 gacccacaaa tggaatcagt cctgcataac ccattgctcg aattacaaaa ggtagttgca
    27841 tattatttat ttgggttcgt agacataaac accgcggccg gtcttacgac ctagatgacc
    27901 tttggcaact agatcaatga gtagcggcga tggacgatat ttatcatcct taaactcggc
    27961 ttggtatacc tccatgatgg ctaagcaggt atctaagcca atgaggtcag caagggccaa
    28021 tggaccaatg ggatgattgc aacccagctt cataccggta tcaatatctt caggactcgc
    28081 aacaccctca tgcaagacga agaacgcttc attaatcatg ggtagaagaa tgcggttcac
    28141 cacaaaaccc ggagagctct gaacggtaat gggttctttg cccatcttag tcgccatagc
    28201 aaggactgct ttgagggtct cggggctcgt ttgcttaccc ataatgacct cgagcaaggc
    28261 catgagtgga ggtgggttaa agaaatgaat tccaataaaa cgctcaggcc ttgagttaag
    28321 agagcccaga tgtgtaatgg agatcgatga agtattgcta gcaataatgg tttctttact
    28381 caccacctga tcgacctgtg ccaagatctt ttctttaatg gcttgattct cggtcgccgc
    28441 ttcaatcacc aatgagaccg aggcaagatc ggtatagagt gtggtggtct taatgcgagc
    28501 caaggtctgt gctttttgct ctgaggtaat cgattctttc ttgatgagac gatctaagct
    28561 tttacttaat gccgctaaac ctttttctaa tgcagcatcg ttgatatcca aaagaatgac
    28621 atcaaaacct gcgccggcag ccacttgagc aatgccattg cccatcgtgc ccgcaccaat
    28681 aataccgatt gatcgtattt ccatacttaa ctctttctgc gtttgtattg gctagcacca
    28741 aagagctgct ctttatcttt cttgtccatc tcgggcttgc gcaagttggt taggatctca
    28801 acagcgcgtt taacagcagg gcgtttccca atcatctcga accaacgctt gaagtgtggg
    28861 tagtcggcca agtccattcc ttgtttatcc caacggcgtg tccatggaaa gatcgccata
    28921 tcagcaatgg tgtattcatt accaacgatg tatttgttct tctcgagacg ttgatctaac
    28981 acaccgtaca aacgctttgc ttcattggta tagcgcttaa ttgcataatc gacttgctcc
    29041 ggagcataca agcggaagtg atggttttgt cccaacatcg gacctaagcc ccccatttgg
    29101 aacatcagcc actccagggt ctcgtactta ccccggattg atttgggcaa gaagcgacct
    29161 gtcttattcg ctaagtacaa caagatggcg ccggattcaa ataaatgaat aggctttcca
    29221 tctggaccct gcggatcaac tatggcgggg atcttattgt tgggactaat ctttaagaac
    29281 tcaggcttga actgatcgcc tttaccaata tcaacaggga ttgcccgcca atcgcgatct
    29341 aaacgatagc cacattcttc gagcatgatg tggatcttat gaccgttcgg tgttgcccaa
    29401 ctgtacacat caatcattgt tttattttgt ttactcatct tggtctctgg tcatgaatgg
    29461 tgttaattat ttaggataga gcttgtaaac gctctaaggc agttcgtaag gtttgctctt
    29521 gtttagcaaa acagaagcgc acgaccccag actcgttctg cttggcataa aaggcagagt
    29581 tcgggattgc cgtaacaccg agctcaatgg tgagccagcg acagaactcc gcttcggata
    29641 acctcgcctc agccataggt aaggcagagt aatctacaca ctgaaagtaa ctaccggtgc
    29701 agggtagtag ttttaactta gtcgacccca aaccttctct aaagagatcc cgcttggttt
    29761 gataaaaacg tggtaagttg agatagtgct ggggctcttg catatagcta gccagggcat
    29821 attgcatcgg ggtattaact gtaaatacat tgaactgatg caccttacga tattcagtca
    29881 tgagagcggc aggtgctgcc acaaaagcaa ccttccagcc cgtgacatga aaggtcttac
    29941 caaaactgga gatcaaaaaa ctgcgtttct gaagttctga atgacgggca atgctctgat
    30001 gcggctcacc gtcatacacc atatgttcat agacctcatc actcaatatc aaaatcgacg
    30061 tgttcttcac aagatcgtag agggtctcaa gatcctctgc agtccagatg ctagccgttg
    30121 gattgtgggg cgtattcgtt aggatcaaac gtgtctttgg attaatcgct gctgcaatcg
    30181 cagcccaagg taaagcgtat gactgcactt gaccattact atcccgaatg agttccatcg
    30241 gtatggagat cgctttgcca cccgctaatt caatggcggg taaataacta tcaaaggcag
    30301 gctcgatcac aatcacttca tcatcacgac caacgcaggc ttgaatggtg gtaaagattg
    30361 cttgcgttgc acccgcagtg acggtgatct cggtattggg gtcgtagtga tgaccataca
    30421 gtgactcaat cttatgggag atcgcttggc gtaattctgg aacgccagcc ataggcggat
    30481 attggttatg accagcccgc atggcatcgt tgacggcatc gagcaaacgt ggctcgcaag
    30541 cgaagtccgg aaagccctga cccaaattaa ttgccccatg ctccagtgca agagctgaca
    30601 tggttgtgaa gatggtggtg ccgatatggg gcagcttgga cggaaacatg gctcccccgc
    30661 gagtactgtc gctagaatga ggggtctgtg ttgcttgctg atgcatggct aaattgtgcc
    30721 atgcagaggc tcacgagtta ggatcgttga taaattgtct gtaaagagcc gtttcacctt
    30781 cttgccattt ctgcgctggc tacccgagta tcaacgccct ggagtggttc gcactgatct
    30841 attggcagga ctaacgggtg caatcgtggt cttgccccaa gggattgcct ttgccttatt
    30901 ggcaggaatg cctccgcact acgggctcta tgccgccatg gtcccttgca tcatcgctgc
    30961 tttatttggt tcaagccgtt tgatggtaac tggccctgct aatgctattt ctctcaccac
    31021 catggccttg attgcgcctc ttgccattcc agagagtcaa cactacgtag cgctggtgct
    31081 caccctgagt ttcttgattg gcgcaataca gattgcatta ggattgggtg gggcagggaa
    31141 atgggttgag aaggtgccgc attccgtgat tgtgggcttt actgctggtg ccgcggtctt
    31201 gattattaat agtcaagtgg ggactctgct tggcattgat attgagcgtg gtaccaaggt
    31261 gatcgatacc attagcaaaa ctttcacctc catacagcac ggccagtggc gccccgaggt
    31321 tttgttgctg gtgctcatca ccttagttgc gatgcgctta tggaaaccac tgaacaaatg
    31381 ggtgcctgcg atgttggtag cggtgattgt gggcagcgta gcacttctcg ttctcgaaaa
    31441 gtactttcct gtctttgatc aaatccggcg ggtgagcgca ataccagggg cattacctcc
    31501 actgtcgttc ccacaactaa cgctcgacca tcttcaactt ctctttggac ccgtcttagt
    31561 gatgacctta ttggcatcaa ccgaagccat ggccattgcg cgcgcaatgg cactaaaacg
    31621 taaagatgcc tttgatgcca atcaagagtt cattgggcag ggctttgcca atgtggccgg
    31681 ctctttcttc tccgcctatc catcgagcgg ctcctttaat cgcacgggtg taaaccttgc
    31741 cgctaatgcc caaacgccac tatctgcaat cgctgccgcc ttgttcttgg tgattatctt
    31801 agcctttgta tctcctttgg ctgagtactt gccctatgca gtcgttgcag cgttactgct
    31861 ggcagtggct tggaacttga ttgatgtaca acaaattcgg catgagatta ggtctgggcc
    31921 acgcgaatgg atacccatgt tgattactgg gcttggcacg atcctgattt ctctggagtg
    31981 ggctgtgctg gtaggtattt gtgtggctgc gattgctaag cgctggcccc aatctgcaaa
    32041 ataatcgcag atgactagaa tagggtgtct acccattttg atgaagattg atttatgttg
    32101 attgttctct cgcctgcgaa gtcactcgat tacgagtcgc ccatttctac caagaaacat
    32161 accttgccag attttgtggc agagtccgcc aaattaattg ctgaccttaa aaagctatca
    32221 ccgcaacagg tctctaaact gatgggcatc tctgacccac tcgctgctct aaacgttggt
    32281 cgctatcggg attggtccaa aacatttacg agtggcaata gccgtcctgc cttgctcgct
    32341 tttaatgggg acgtctatga aggttttgat gccaagtctt tagatagcaa ggcccttgat
    32401 tttgcccaag agcatgttcg tattctgtcg ggtctctatg gagtactgcg ccccttagac
    32461 ctaatgcaag cctatcgcct cgagatgggc acggcattca agaacgctcg tggtaaggat
    32521 ttatatgcct tttggggcga tcgtattacc aaagcaattc ataaagacct caagaaacaa
    32581 aagaaaccgt tcttacttaa tctagcatcg gaagagtann nnnnnnnnnn nnnnnnnnnn
    32641 nnnnggtaaa tacaaaatca tctcctttta tgccaaacgc gcacgcggct tgatggcccg
    32701 ttttgtagtg gagaaccgca ttaccgatcc ggccgatctc aagaacttca agaccgaggg
    32761 ctatcgctat agtgctgcgg agtctaagcc cgataagccc gtatttcgtc gggcagagca
    32821 aaagaagtaa agtagattct tcgaagagag taattacgct atggcagtcc atcgttctaa
    32881 atccgtacaa cgtatttctc ctgagtctga gcgcttggtg gctgactcca tctcattggc
    32941 agcctcgggt agtcagctcg aggatcgctt ttgggaagag cgtttgcaca atcgcttact
    33001 caagctctta aaaagccatc atcaaaatat tattgataca gcgcttgatc aaacctttcg
    33061 gattaatacg gttgcctttg aggttcttgc tgatactgca gagaccctcg cggagtcgat
    33121 gagtcttgag catgatggta aaacgtggga tgccttattg ttagcgatgc ccatcgtggc
    33181 acacacgcgt tatcaaattc catcggggcc tctaccagta agcatcgtag aggcagcggc
    33241 agcggcaatg caaaagtcga ttgcggcaga gggaacacaa ctcgccatca tcccttggct
    33301 ctacagcatt gaccagatgc cgcactcgca tactcaaact cgctcactct tagagacctt
    33361 agcaaacgcc gccgtatcgg gtggtgatat gaaattagag ttgcgcgata tgtctgagac
    33421 tattgctgta ttagcagacc cgcgttttat tctggcagtg atcgtagcgc ctaaggatca
    33481 gccaatcttc cgttggcaaa tggatggacc acaacgtcaa gagcggggcg tgagtttgat
    33541 tgagtggcag accgcgatgt atgagccgct ggtgagctta ctgcctggct gtgagtttga
    33601 attacttctg cccgaggctt acttcacgaa ctgtcgttta gcagataaac acgtgcgccc
    33661 actcagcatt cgggctgcta tcaacttcct cgaatccaca ctcggcgtac taccagccgg
    33721 cttatccgcc gtcgttggtg cttttgggga agagcaagcc gatgagtacc gtattgcatt
    33781 tagtcttaaa ggatcatctg aggtcattta tggagtgatc tggccactct atgatcgcga
    33841 gaccgtctca agcgatggct tgagtgatgt atcagatgag gagagtccga tcaaacggat
    33901 ttgtgatgcc ttgcacgatg ctggcgtaga agatgtattt cgtcacgccg tattgttctc
    33961 accagagctg tgtgatgatt gtggggcgcc actgtttcca gatcggcagg gtgaagtggt
    34021 tcatgctgag atgccagaag atagcccctc gcaacaaccg ttatttcatt aatttatttc
    34081 tcaagcgcta gaaggtgcgc ttctttacct tctttcattg ctacggtctt gcattcatcg
    34141 gcgagtcgat taccggtctt gatattaaag agcattgcct taggaccaat tacaactaaa
    34201 tcgagaccgc tgtcatggtg tttaaatcgc atgctgccag gtaaagatgt ttctttctgc
    34261 atctcatagg acttggagcg ccattgcacc tgtaatttat tgacaggtcc actggtaatg
    34321 aagcgttcat tgttactaca actccaaata tgcggaatgg tcagggagct ctcttgcgcc
    34381 agaaccaagc ttggtgaaca cagcgcaagg caaaggccta gaccgcttac aaacttgctt
    34441 ggtaataaac gcatggaacc tcccttagac caatctatct taactcagaa ggtgctttac
    34501 tcccgcttgc tcttcgagta attcatttaa ggtaagggtc atacgctcac gtgagaacgc
    34561 gtcaacctcc aagcccttaa tcagggtgta ttcgccattg gcgcaggtaa ccggaaagcc
    34621 ataaattacc tcggcaggga tctcataatc accatgtgaa gcaataccca tcgtgaccca
    34681 cttaccattg gtacctaaga cccagtccct tacatgatcg atggcggcat tcgcagcaga
    34741 cgctgcagag gagaggccgc gcgcttcaat aatcgctgca ccacgcttac caactgttgg
    34801 gataaagaca tctttattcc acgcagcatc attaatcatg tctttgaccg actggccatt
    34861 gaccgttgca aagcgatagt cgggatacat cgtagggctg tgattacccc aaacaatgag
    34921 tttctcgata tcagcaacgg ctttgttggt cttactagcc aactgcgata aggcacgatt
    34981 gtgatcgagg cgcaacatgg cagtgaagtt cttagaaggc aagctaggcg ctgatttcat
    35041 ggcgatgtat gcgttggtat tagctggatt gccaaccact aagaccttga cagtacgttt
    35101 agcaacttgg tctaaggcct tgccttgcgc tgtaaagatc tgtgcattgg cagagagcaa
    35161 atctttgcgc tccatgccgg ggccacgagg acgggcgccg accagaaggg caacgtcaat
    35221 atccttgaat gcggtcattg gatcgctgtg cgcgctcata cccgctagca atggaaaggc
    35281 acagtcctcg atttccatca tcacgccctt aagagctttc tgcgctttct catcggcaat
    35341 ctctaataac tgcaagataa cgggttgatc tttgcccaac atatcgccat tggcaatacg
    35401 aaataaaagg gaataaccaa tttgaccagc tgcaccagtc acagcgacac gcataggggc
    35461 ttttgccatt acaaactccg gataaataac gttaaaaaga gaaacagaac cgctcaaaaa
    35521 cagtagttta catttacact tgcactaagt cttctataag ataaatgatt aaaatatttc
    35581 atcatgcgac cgtttgagca atccgctagt tttagccccc tttaccagca gatcaaagaa
    35641 ctgattttag gaagcttgca ggctggtgag tggcaacccg gtcaagccat tccgagtgaa
    35701 atggacttag cggagcgcta taaggtgagt cagggaacgg tccgcaaggc catcgacgca
    35761 ttagcctcag aaaacctagt agtgcgtcat caaggtaaag gcacatttgt agccagccat
    35821 cgcgaagagg cggtgcaata ccgcttctta cgattagtgc cggacgatgc caaggaacta
    35881 atcctaaaga gcaagttctt aagttgcaag gaagtgcctg ccaatgaaag ccttgcgaag
    35941 caatttaata tcaagcacaa agatccactt gttctgatca agcgcgtgca gtccttccaa
    36001 ggcaggcccg ttgtctatga gtcaatttat cttctagcca cgcgttttaa aggtattacg
    36061 cttgagcgtc tcaccagttg gcctggtccc ttgtatggct tctacgaaac agagtttgca
    36121 acccatatgg tgcgcgcaga agaaagcatc aaagcactag gcgccgaccc gctagtggct
    36181 cagcatcttt tgctcgaaga gtcagccccg gtattacagg tggagcgtag aacctttacc
    36241 tatggcaata agaccgttga gattcggttg gcgcactatg agaccaagga tttgcactat
    36301 gccaatgacc tcaattaggg ctaattacta cgcataaata cctaatgtac aaaatataaa
    36361 tagctataga atcaatatgt taacgatcgc aactgaaaaa atgtccactt caccttacgt
    36421 ttctactaga atatgttgca acacagcaat aattaacaat ttggccttat taagtcagga
    36481 ttacaaccag ctatgagtga ctcccatcag gacgcaaata cagcccagcc tcggccggtc
    36541 ttccgtaata ttggcatcac acaattagcg caatatcgcc tcccttggcc tggcaaagtg
    36601 tcgattctgc atcgcatctc cggtgccgct ttatttctgt ttctcccatt tattttgttc
    36661 ttattcgata agagcctagc ctctgaagtg agctttgctt actttgctaa tctcatgggc
    36721 aatcccatca ttcaattaat ttgtctggga ttaatctggg gatttattca tcatttctgt
    36781 gctggcatcc gctatctttt cttagatctt gagatgggtg ttgataaagc tgccgctaat
    36841 cgttcagcga ttagtgtgat gattgttagt ttggcattga ccgcgatctt gggtctgcgc
    36901 ctctttggtg tgttctaagg agaggatcag aacatgtcaa aacataatat tgggccaaag
    36961 cgtttagtgg ttggagcgca ttacggttta aaagaatgga tgattcagcg gataaccgcc
    37021 atagtaatgg tggttttcac catcgtgcta ctgattgcct acatcgtgaa cggcaacccc
    37081 agctacgatg gttgggcagg cttatttgca aatcagctca tgaaggtctt aaccctctta
    37141 gctctcttaa gtcttttcta tcacgcctgg attggggtgc gcgatatctg gatggattat
    37201 gtaaaaccgg ttggtattcg tttaacgctc caagtgctca cagtattgtg gcttgtgggt
    37261 tgcgctgcgt atgccattca aatcttatgg aaagtgtaag gaatgacagc cattaaaaaa
    37321 tcacttccac gccggcgctt tgatgcggtg attgtggggg caggtggctc tggtatgcgc
    37381 gctgcattgc agctatcaga ggctggttta agtgttgctg ttcttagtaa ggtgttccca
    37441 acccgctcgc acaccgttgc tgcgcagggt ggtattggcg catcgcttgg aaacatgagt
    37501 gaagacaatt ggcactatca cttctacgac acagtcaaag gctcagactg gttaggcgat
    37561 caagacgcaa ttgagttcat gtgtcgtgaa gcaccaaagg ttgtctatga gttagagcac
    37621 tttggcatgc cctttgatcg taacgccgat ggcacgatct accagcgtcc cttcggtggt
    37681 cacaccgcca actatggtga gaaacccgta caacgagcat gtgctgctgc agaccgcaca
    37741 ggacatgcga tgttacacac gctgtatcag cgcaacattc gtgcaaaaac ccatttcttt
    37801 gtagagtggt tagcactcga tttaatccgg gatgcgcagg gtgatgtggt tggtgtcacc
    37861 gcgcttgaga tggagaccgg tgaagtttat attcttgagg ctaagattac tttgatggca
    37921 accggtggag caggacggat ttggtctgca tccaccaatg cttatatcaa tacaggtgat
    37981 ggcatgggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgctgac
    38041 cgaaggatgt cgcggtgaag gcggcattct gcgcaataaa aatggtgagc gctttatgga
    38101 acgctatgcg ccaaccttaa aagatcttgc accgcgtgac tttgtatcgc gctcgatgga
    38161 tcaagagatt aaagagggcc gtggttgtgg tccaaacggt gactacattg ttctggatct
    38221 gacccacatt ggtgctgaaa ccatcatgaa gcgtttgcca tcggtctatg agatcggtat
    38281 gaactttgct aacgtggatg tcaccaaaga gcc
//
LOCUS       DDPQ01000132            8724 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_4682,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000132 DDPQ01000000
VERSION     DDPQ01000132.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8724)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_4682"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT         2374 a         2227 c         1791 g         2332 t
ORIGIN      
        1 tcccctcaat cgcagcctct atactcgatt cgcctgaaga ccatagctct ccaaaagctc
       61 gcattgcagg caaatgagtg aagccatctg tcttgcatcg ttgtactcgc ccaccttcgt
      121 gatcaataca aattaggata tctttacgaa ctgccataat ttgattagtt aacttcatta
      181 gctgagagcg agatgtaaag ttacgtccaa ataaaatgac gccacccgtt aaagggtgtg
      241 agattcgttc ttcatcccgc cgacttagct ctaagccctc aacatccaaa ataacggggc
      301 ctggatttcg attgtcttgc tcattactcc gcatgcttaa taatcatcct atgtagttgc
      361 ttggatcaaa agtactaaca atgggggtac cataactcac caccacaaat gccatcacca
      421 tatcttgctc atcagtcacg gttacttgag cgtgccattg cttctcaccc ataaagtctg
      481 ccaatgcgcc cgagtacttg gtataaggtt taccgctagg ctcattgaga gtctgtaggg
      541 acctccaggt catgggtgta tgcattccca agccaatcgc cttagaaaag gcttcttttg
      601 ctgcaaaacg agtggctaaa taggcaatac cacgtttgtg attacgtttt agcctttgct
      661 caaagacaat gagttcctcg tccccgagta ctcgctttgc caaacggcct tgtgtccgtt
      721 cataggctgc ctctagccgt tttaattgca acaaatctgc gcctattccg acgatcattg
      781 tgcgcgagcc tcattcatca aatggcgcat atcttggata gccaaagccc aacccttaaa
      841 aaccgcctct gcgacgatgg catgcccaat attaagctca ctaatctcct gtatttgagc
      901 aatggctttg acattccctt catttagacc atgaccagca ttgacacgaa atcccaatga
      961 ctttgcgtaa tgggccgctt cctggattcg ttttaactgc tgggtctgcg tagactgatt
     1021 ggcatctgca taggcaccgg tatgtagttc aataacatgc gcacccactt cttttgctgc
     1081 atcgatttgt tcactgtcag gatcaataaa caatgacacc cgaataccag cctctgtcaa
     1141 ttgagtaatg gcagatttaa cttctgcagc atgaccgata acatctaacc caccctctgt
     1201 agttatctca gttcgctttt cggggactaa acaaacatca tgaggtctaa ccttcactgc
     1261 gatttctaac atttctgggg tgatagcaca ctctaagttc atgcgcgttt tgatcagtgg
     1321 acgtaaggca aatagatctg cgtccttaat atgtcgacgg tcctcccgta aatgcaaggt
     1381 gatgagatcg gccccagcct cttcggcaag acgtgcagcc aatatagggt ccggatatga
     1441 agtaccacgc gcattgcgca aggttgcgac atgatcgata ttgattccga gctcaagaga
     1501 ggacatacct aattctaaag gcataaatct cgcttagatc tttttaagat caattaatat
     1561 ttggcgtgtg gttaattgtt gatcttgcag atgaattcct aataagaaac gcatgagctg
     1621 tttactttgt gtcaaggttt ctgcgtctga gaaatcccca tttgccattg cctccaaagc
     1681 caatcctgtc aagacaggcc aatgcccagg gtcatcactt tgaagggggc gtatcccctt
     1741 ctcgggttga tacacatagc gttctgttcc aacaggtttg gtttgggtct cgacacaatg
     1801 atcgagagcg gctgcatagc ctgtctctcg taacaatgct aactcaaagg gccttagtgt
     1861 ctgctcaatg cctgcgcctg actccataat ttgggacaag gcatatacgg tacttgcgta
     1921 ctgatcatag agcgtctcat aggcatcttc tcgcgccaag aacttcacta aaagctcatt
     1981 gaggtaaaac ccgcacaaca gtgcatcacc gactaatgcg ggcgagcctc caacccattc
     2041 tgatttcgtt aaggttcgaa gttctgactt gccactccaa gaaatgagta agggctgaaa
     2101 gcgctgtaac actgggcgta aactagaatg aggtcgtttg gccccttttg caattaaggc
     2161 aagccgccca tgagagcggg taaatacatc gagaatgagg cttgtttcct tgtagggaat
     2221 gctatggagc acaaaaccag gctcatctaa gacacgtgtt ccagccattt attccaaacc
     2281 ttgcgcccgt agctctgcca aatcatccgc ccagccccgc tttactttga cccaagtttc
     2341 taaaaacact ttgccatcaa acagtttttc catatctaag cgggcttcgg ttgaaatctt
     2401 ttttaatcgc tcgccttttt gaccgatgat cattgctttg tgactatcgc gatctaccaa
     2461 aatagtcgcg gctattcgtc gcatcttacc gtctaactta aattgatcaa ttactaccga
     2521 gcttgcatag ggtaattcat cgccggtata ccgaaatacc ttctcacgca aaatctcggc
     2581 ggccataaag cgctcactac gatcggttaa ggtttcagca tcatatacag cctcaccctc
     2641 tggcaaatgc tcttcgataa tatcgagcaa gcgcccaata tcatctttgt tcttgccgct
     2701 catcgggatg atctcagcaa actcgcctag aggcgatgaa atactttcag actcaaaacc
     2761 cttggtttga gctgcgattt ctccccaagt ttggcccatc ttccccatga actcaaataa
     2821 ggcggcatca cgatcttgtg ggatctcaaa gcgcgatgca aataaatcta atttgttagc
     2881 aatcaataaa actggcttat ccgatggcaa caaacgtaat acttttgcat catccgcacc
     2941 ccaataccct gcctccacaa taaaacagat gacatcgaca tcgttcaagg tgcttgtgac
     3001 agtacgattc aatgtttgat ttaaggtatt catcacccgc gtctgaaacc cgggcgtatc
     3061 aataaatata aattgcgcac tgtcgcgtgt ctgcacacca gcaatgcgat atcgggtggt
     3121 ctgtgctttg cgtgaggtaa tgctaatttt ttgaccaacc aaggcattca acaaagttga
     3181 tttgcccata ttgggtctgc ctaccaatgc gatcgtgccg catttaaaca tcactaatcc
     3241 tttagcttca ggggcagctg gcccttacca gtatcggctt ttgctgcttt tttcttggca
     3301 gcgcgggtct tctttggctt acgactttct tgcggtagcg ccttttgaac agcagccagt
     3361 gctaatttag cagcgacttg ctctgcagca cgccgcgagg caccttcgcc cttaacgttt
     3421 accttcagat tcggaatggc gcactcaact tcaaattgct gattgtgtgc agcccctgat
     3481 gtgccaacca cgttgtatgc agggagtggt aactgaaatc cctgtaaata ttcttgcaac
     3541 aatgttttat cgtccttacc caaggtttta ggatcgacat tttgcaaaat gctcgaatac
     3601 aacttacgca aacttgcttt agcagcatca aaaccaccat caataaaaat tgcgcctgtg
     3661 atcgcctcta aggtatccgc caaaatagat ggtctgcgaa agccaccgct ttttaactca
     3721 ccctcgccta actttaagta atcagatagc aacaatcctt gagcaatttc atagagagcc
     3781 tgctgcttga ctaaattagc gcgcaccctc gataggtcgc cctcatctaa atctgaataa
     3841 cgttcataca aaatttcagc aaccgcacag ttcaatacag agtcccccaa aaactcaagg
     3901 cgctcattat tctttttgct atgacttcga tgtgttaaag cctgatttaa gagctctggc
     3961 ttcttaaacg gatagcccaa gcgctcctga agagggccta gatcaagagt cgcgcgagcg
     4021 ttcatatcta ctgaaaactt ccaatacgtc ccaaggagcc catattgagc caaataaaga
     4081 aggctcgccc tactaaattt tgatccggca caaatcccca gaatctagaa tccaaactgt
     4141 tatcccggtt atcacccatc acaaaataat ggcctggagg caccttgcaa ctcatcgaag
     4201 tgccaatgta ttggcaaaac tctgcgcctg gaaaacgctc gggttgataa attgtgctgc
     4261 gatcaggatc gttcaaaatc tcatgttgat taccgcccaa atccgttgga aaactttcct
     4321 tgtagaaatt agcgtaacgc atgctctctg gatccaggta tggagtgcca cctgcatata
     4381 aaagaggctt gccattaata actacttttt tgtcctgata tacgatctca tcccccggaa
     4441 ttgcaataac gcgtttgatg taatcgaccg actcatcttt gggatatcga aagacaacca
     4501 catcgccccg ttttggggta cctacatcaa gcactttttt gttgatcact ggcaaacgga
     4561 ttccataggt atatttatta accacaataa aatcacctat gtgtaaagta ggaatcattg
     4621 agcccgaggg gatcttaaaa ggctcaatta aaaatgagcg taggacaaat acagccacaa
     4681 tgactggaaa gaaacttgcc gagtactcaa gccataatgg catgcgctct attcccgcag
     4741 ctttgcgttg cggagcaaag taaagcttgt cggcaatcca agcaatgccg gtaatcacca
     4801 ctaaggtaaa tagaattaaa gcgaagttca tttttcctct acctgcaaaa tggctaagaa
     4861 agcttcctgt ggaatctcca cattaccaac ttgcttcatg cgcttcttac cttctttttg
     4921 tttctctagt aacttgcgct tacgcgagat atctccacca tagcatttag ctaaaacgtt
     4981 cttgcgtaat gccttgacgt tctcacgagc aataatattg ctaccaatag ctgcctgaat
     5041 ggcaacatca aacatttgcc ttggaataat gccgcgcatt tttgcaacca cctcacgacc
     5101 tcgatgctga ctattgctgc gatgaacaat gatcgataaa gcgtccactc gatcaccatt
     5161 aatcaaaata tctactttca ctacatcagc cggacgatat tctttaaact cataatccat
     5221 cgatgcataa ccacgggaga ccgatttcag gcgatcaaag aaatccaaca caatttctgc
     5281 cattggtaac tcgtaggtga gctgtacttg acgacccaaa taggtcatat tagtttgaac
     5341 tccacgcttg ccagtacaca aagtaatcac cgaaccaaca tactcttgtg gcatgtagag
     5401 attaacggtc acaataggct ctaagatagt ttcaatcttg ctgggatcgg gcatcttgga
     5461 tgggttatca accacgattg ttgtgccatc gcgctgcaag acttcgtaca ccacagttgg
     5521 tgcagtagta attaaactca tgtcaaactg acgctctagg cgctcttgca cgatctccat
     5581 atgcagaagc cccaaaaatc cacaccgaaa tccaaagcct agagcttggg agacctcggg
     5641 gtcgtattgc aaagaagcgt cattgagttt gagtttctca agagattcgc gcagagcgtc
     5701 atattgattc gcctcgacag gataaagccc tgcaaacacc tgaggtttaa cttctttaaa
     5761 tccaggaaga ggctctgctg cgggagtctt gccttgttga ccagtggcat gcgtcacagt
     5821 atcaccaact tttgcagctt taagttcttt aatgccggag ataataaagc ccacctgccc
     5881 agcagaaagc tcactgcgag ttaatgactt cggtgtaaaa acacctactt gctcgactaa
     5941 atgggtagag ccggttgcca ttaacaaaat tttgtctttt ggtttgagag tgccgttcat
     6001 tacccgaacc aacatcacaa cacctacata attatcaaac caagaatcaa taattaaagc
     6061 ttgcaatggt ttagtaacat cgccttttgg aggcggtaca cgccgaatca tctcttctaa
     6121 aacatcagca acacctaaac ctgtttttgc tgaacacgtt accgcatcac tcgcatcaat
     6181 accaataaca tcctcaattt ctttcttggc cgactctggg tctgctgatg gcagatcaat
     6241 tttgttgaga ataggaataa cctctactcc aagctcggtg gcggtatagc aatttgcgac
     6301 agtttgggcc tcaacaccct gactggcgtc aaccactagt aatgcgccct cacaggccga
     6361 taaagaacgg ctaacctcat aagagaaatc cacatgccct ggggtgtcga tcaaattaag
     6421 gttgtaggtc ttgccgtctt tggctttata agatagggct gcagtttggg ctttgatcgt
     6481 aatcccccgc tcgcgctcaa tatccatgga atcaaggact tgctcaccca tctcccgatc
     6541 ggataagccc ccgcataact gaataattcg gtccgccaag gttgatttgc catgatcaat
     6601 atgggcgatg atggagaaat tgcggatgtg atccattggc tgttctatag gtgttgtaca
     6661 aaacgccttg tcgcaacgca acacaatgat gcgctgcaat aagatgtctt tcctccattg
     6721 taatgggata gggaaaaggg cttattttcc ttatctgcag gggatattac ccctgcaccc
     6781 atgaaaaaag ggtgtctttc aacacccttt ttgaacctaa aactatgcgc ttatttttga
     6841 gctggtcgaa ttgggaccac aatcgtgcca tccccccgtc gaacaaagac tggaactgat
     6901 ttattaggat ccatcgcctt tactgcggac tcaaactgtt ttacgccagt aatatcagta
     6961 tcgccaatcc gcacgatgac atcaccaact cgtaagcctg ctcgaccggc tgcgccctca
     7021 ccaacattgg taatttccac cccacccctg ctaccaagat ctttcttctt tgcatccgag
     7081 acatcagaaa cactgatacc tagggacttg tttgcagtac cagcttgtgg acttggggtc
     7141 tccactttct tagcaaccgc cttatcgggt tcagcatcgg ctacaacaac cgttagatca
     7201 cgggttgtac ccttacgcca aactttgaca gtagcactag tgcctggctt agtatcgcca
     7261 acaatacggg gtaaatcagt agacttagta atctctttac cattgaagct cagaatcaca
     7321 tcaccggcct caataccacc ctgtgcagct ggcgcacctg gctcagcatt acgaacaaat
     7381 gcgccacgag gttttcctaa accaagactc tcggcaacct ctttattgat ttcgccaatc
     7441 gccacgccaa ttcgaccgcg cactaaacgt cctgtagtgc gaagctgatc agcaacgcgc
     7501 atggcctcat cgatggggat cgcaaatgag attcccatat atccacccga gcgactaaag
     7561 atttgagagt taatcccaat tacctgaccg gcagtattaa gaagaggccc ccctgaatta
     7621 ccagggttta cagcaacgtc cgtctgaata aatggcaaat aatcaccggt gtcacgactc
     7681 ttggccgaca caatgcctgc tgttactgta ttttctaaac caaagggtga accaatcgca
     7741 acaacccact cacctactct caccttagac gagtcaccga gaggtaactt gggtaaacca
     7801 gttgaatcaa tctttaaaac ggctaggtcg gttcgcttat cggcgcccaa taactttgcc
     7861 ttgaactcgc gcttgtcagt tagggtgaca taaatagtgg tcgcaccttc aaccacgtga
     7921 gcgttggtaa gcactacacc cgaggagtcg ataatgaacc ctgagcctac cccgcgctca
     7981 atctcttcag gttgaccccg acgattttgt ggtcccttgg ggccacccgg cgctccaggt
     8041 aatggaacac caaagaagcg acgaaagaat tcagcttgct catcaggaat accgggggcg
     8101 ccgccctgag cctgctgagt agatggcttc tcggtggtgc ggatatttac aaccgcaggg
     8161 cttgcctttt cgactaaatc aacaaagtcg ggcaaagcaa cccgaggagg tgtttgagcc
     8221 tgcgttatag gagcaaaaga taggaccccc cagctaaaga ctgaaaagac cgctaatagg
     8281 atttttttca taagaaattg agcttaactc aatccaatag ttgcaatact gacaatatta
     8341 gggcgtattc ccaaatatca aggtaccgtt agtaccccca aatccaaaat tgtttttaac
     8401 cgcgtagcta atcttcagat ccctagctgc attagcgcag tagtctaaat cacattctgg
     8461 gtcctgatta aagatattaa tagtaggcgg tgacttttga tggtgcagtg caagaactgt
     8521 aaatacggac tctaagccac cagcgccacc caaaaggtgt ccagtcatag atttagtgga
     8581 gttcacaact acttttttag catgatcacc taaagccgct ttaatcgcct cggtttcatt
     8641 tttatcaccc aaaggagttg atgtgccgtg cgcattgaca tattgaattt gatcggcatt
     8701 taaaccagca tctcgtaagg catt
//
LOCUS       DDPQ01000133           22133 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_12651,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000133 DDPQ01000000
VERSION     DDPQ01000133.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 22133)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22133)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22133
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_12651"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    5518..5543
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5778..5897
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9817..9886
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17787..17815
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19134..19196
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20195..20308
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21797..21837
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6008 a         4731 c         5113 g         5811 t
ORIGIN      
        1 gatacgatta aatcgcaaat gagcgcaaaa gatggcacag ggaatgcaat attcccagaa
       61 acgattaatc aggtccaaat agaacctaac gttacagtag gctcaggagt tcagttcacg
      121 gatgcattta atggcggaac cctagccgac ccaacacaat cagcgctcta cataaaaatc
      181 aatgattttg tgacctactt agatcagcga gcaactggct cggcccttac aaacagtgcc
      241 agtacagtgg aatctggctt aaatgacata tttgatagaa tcaccacagc acaacaaaaa
      301 agtggcggta tagcggcaca ggttgatata tccaaagagg caatgtctaa gattggcaca
      361 gaaatggcag ccaccgcatc ggcgttattg gataccaatt tggctgaggc cacggctgcg
      421 tacacccgat cacaaacttt gttaaatgct gcccaggcga tgtttgctcg tttgcaacag
      481 agctccttgt tttcaaaact gtagtttttg gtgaaataaa cgtatgaata ttccgattgg
      541 ttggattatc gcgatgggtt gtgccctagg gggctatgcc ctccatggcg gaaacatcat
      601 ggttttatgg caacctaccg aagtgttgac cattgtaggt gctgctgttg gcaccatgat
      661 tgcttcaaac agcatcatta acctgaagaa gacctttgcg gctctcggga gcgcctttaa
      721 aactaaatca tccgtcaaac agatgcactt agatttattg tgcttaatgt ttgagatttt
      781 gcaaaagatt aagcgtgatg gtttgatgtc tttagagggt gatattgaag aaccagaggc
      841 gagccctctg tttgaaaaat atccttcagt caccaaggat caccacttgg tagatttcat
      901 tacggattac ttaagaatga tgctgggcgg ttccttagac gttatccaaa ttgagagctt
      961 gatggagcaa gagttagacg tccatcatca agaaggtcac attccagttg cttctgttac
     1021 aaacgtcggt gacggtttac cagcgttcgg aattgttgct gcggtgatgg gtgtggtaca
     1081 taccatgggt tctattggcc ttcctcctgc tgaactcggt aagcttattg gtgccgcact
     1141 ggttggtacc ttcttaggta ttttgttggc ctacgctttc gtatcaccgg ttgctaaagt
     1201 attagagcaa aacctcgaag cggatacacg ttcatacatg gcagtaaagg ccattttgat
     1261 tgctagcttg aataatttcc cacctgcagc agctgttgaa tttggacgca aggtattgtt
     1321 cacctatcaa cgtcctactt ttgcagagtt ggatgagggt acaaaagcag ttaaaggaaa
     1381 atagtcatgg ccaaaaatga cgcgccgcca attgttatca agcgcgttaa aaaaggcggt
     1441 catggacatc acggaggcgc ttggaagatt gcatatgccg acttcgtgac ggcaatgatg
     1501 gccttcttcc tgctcatgtg ggttctaggc tctactacag caggtgatct cgcaggtatc
     1561 tcatcgtatt ttcaaaaccc attgcgggtc tctttgtctg gcgggcaggg atcgggtgat
     1621 gcaacccgca tcattaaagg tggtggcgac aacatatcaa aaaccgttgg tgaggaaagt
     1681 cgcgccgatg cggatactga gcaacgcagg attagcgatt cttcagtcac cgaaattgag
     1741 aatgcccgta aggatcgaac caaaaatgaa atggttcgtg atgacattaa taaacaggtc
     1801 gattctgatc cagagttaaa aaatgctaag ggtcagattt ttatggatat ctcagccgag
     1861 ggcttgagaa ttcaggtggt ggatgagaaa aataaaccgc tctttgcctc tggcagtgcc
     1921 aatccgacgc cgtctgcaaa acgtttacta cgcattattg gtagcgcatt aaaaagctca
     1981 cccaatacga ttcgaattga ggggcatacc gacaatactc gctatagcac caatgctagt
     2041 tatagtaatt gggagctatc gacagaaaga gcactcgccg cacgacgtga gatggtggct
     2101 ggaggcttaa atgagtcaaa agtgtctcag gtgattggtt ttgcagatac cattccatta
     2161 aacacccaag atcccaagga tcccctaaat cgacggattt cgataacgat cctcaataag
     2221 aaaccgataa acaatgccga taagactgct tatgaaaaag caagggctga aaagctaccg
     2281 gacaatgtaa agcctggcac acaagagatt cctccaaact taagggcgcc ggcacaattt
     2341 atgcctaagg aagagccaaa gaaagctccg cctccaccga aggaagagct taaaaaagta
     2401 gctccaccac caaagccagc gcctgagcct gttaagcctg ccgcccaaga gattcctcca
     2461 aacttaaggg cgcccgcaca attagcgcca aaggaagagg aaaagaaatc gccacgtaat
     2521 ccgcagctag agttgccagc aaaaccagct caaaaaagcg acagcttggg taagtaagcc
     2581 atgaacatca agatcgcact gtttgtattc gcactgttta ttaatagctc tgcttggtct
     2641 gccgaaaaaa aggcagtacc ttctgctgaa ccgactcttg ccgcgggggt agaaaaaaca
     2701 aaagcaaaaa cgcaaagcga agcagaaaag ctataccaac gttatcaacg cctctatgga
     2761 aagatgattc ttgccgttgc gtgtgtacaa aatccaaagt gtattcagcc tgaagatgag
     2821 gtgattaaat acaatatggc catcattcgg gtcacggatc gcctcgatga gttagtcatt
     2881 aagcaaaaag atttggatgc aagctattac cgtggcttga ttgcgtatga gcgtggcaaa
     2941 tactatgtgg gtagagccac cttaatcaca gacccagact ttatcttatc tgcaactgta
     3001 tttcgtcgtt acgcccttga ccagttccgg cttgctgaaa agaatttagc aattaatgct
     3061 gccagtaaaa atccagatgc atgtaaacac cttggtgaaa tcgcagatct tggttatctt
     3121 gggataaaga ataaagacaa ggctacagac tattattact gcgctgcgat gggctatctt
     3181 gatgttggta agaagaatgc tgcagccgat atgtacaatg ccatgagaaa ctcagcaatc
     3241 cataatgatc cgcgtacagt agaggtatat gcgcgtcttc acaataatga tactgtttct
     3301 aatatgcgca aatcagttag ccagaccagt cgtttagatt cagaaacaag taaaatttct
     3361 cgccaataag gagagtatta agatgaatcc aaaagtagat atgttgattg gctatctcaa
     3421 aaagctgctg atttggttag tagctccatt ggtattcatc gtgtttattt ttttccttgg
     3481 cgaacaatat gaggttggtc gactttctgc cgtagcaagt tgcgagggta gcgttaatac
     3541 ccaatcgtgt attcgcagta aaggctattt ggccaatgag cctttttatc ccgacctagg
     3601 ccgccgttat attggtggat ttgcacgctt agtgggggga gaccttggtg ctagctatcg
     3661 tgtagaaccc aaagtggctc cattagtcaa tgctaagcca gctggtgata atgcagaaga
     3721 agatgaggat ctgaaagatc tgccacctaa agcagctgac acagcagcac ccaaagcgcc
     3781 agcaaagaag taatgacaat acggatttca gagtaaatca agatgcgtag tttattgatt
     3841 attgtcatca gcctcttttt agttggctgc tcagattatc aacgcgctcg atcggcttat
     3901 gaaggcggtg attataaaaa ggcgtttgag ctttttaaga aactatctga gtccggtgac
     3961 acccgttcag aatatgacct ttccttgatg tacttgcaag ggattggtac caaacaagat
     4021 gttggtcaag gttggttttg gatgattaag tcagccgagc atggcaatac gatggcaatg
     4081 gttgagttgg gcgggcggca tgagtatggg gttaacttgg acaaagatga gacccaagcc
     4141 gtcttttggt acaaaaaagc cgcccttgct ggcagttcgg ttgggcgtta taacctggca
     4201 aaaatgtata ttgacggtag gggggtagag aaggaccctg cgcgtggata tgcctggctg
     4261 gtcttatctg ctcaaaaggg caatccaatt gctaaagccg aggctgacgc ctacaaggct
     4321 aaattaagcg ctcaggaaca aattcggtgt gatcaggtgt taaagcagct tgaagcagac
     4381 ccaaattcgt agaaatcgat acaaatcaat aagttacgaa atattgacca aggtttaatt
     4441 gatagccttt gggcgcatgc accatttttg gacattttgc gctagaaaac cccgtatttt
     4501 tttgatataa atacagtcct aaagaatatt gggttaccgc aatgaatgaa gtacagtttt
     4561 ctgaaaccaa ctatttacgc ttaacgagta tcttatgctt ggggatcgcc gatattttat
     4621 tggtagccga cctctggtgg atgtttttca acctatttgt catcgcattg ctttcggagc
     4681 ttttacgtaa gcgcctttac gaggcaatgc tgtacaacta tgaggttgag aatcgcaatc
     4741 gcaagctcaa acaaatgaaa gagcgggccg atcaattgat gaaaatgaaa aaagaggccg
     4801 gtgtagctta gtattgtcct tttaatcaga aagtattctt ttgggactct ttaacgctgt
     4861 attgggtggt aaatctggcg cagacataat gtgccaggct ttattcaatg tcgtcgcttt
     4921 tttgccatat tgctctcgtg ccaactatca ggacttcaac gatttttggc atgccaaggt
     4981 caagaatgca aatggggcgg aggagcgcct caaccaaatc ttctttaatt acagcgccca
     5041 ctttacaaaa gacgagcaac tcttaaaaca gctctctcaa gccactcttg agatcgaaaa
     5101 atactggatt agcaaatacg gcttatcggg tcgatcaatt gctttgtttt cagtacttaa
     5161 aaagttggcc gaccaatatc ttccagatgg aacaaaagcg caggaatcgg atttcatgca
     5221 tggcgcatcg catcccaacc ccgagacaag ggtagcgcaa tgtatgcttg atttaagggc
     5281 ctttgaggct ctgttgccca ctttggcacc gactgagatc tcaccagaag agcgcactca
     5341 cttttttgta gctgatttat atgaaaatgc ggtatcgcat tacaaataca gcggcagtga
     5401 tttaaaggtt tatgaagaag attcttattt cattgacagc caaacttatt gggggcgttg
     5461 gggcttattc agaaacagca cacttttaac cgatacttgg attgtcttaa tcaagaannn
     5521 nnnnnnnnnn nnnnnnnnnn nnnctaaaaa aattgagatt gacccagaga ttttggcggc
     5581 gatctcaaaa acgaagagta ctggtgtaga gaatgcagct tcatttggaa tgccaccaaa
     5641 ataccttcaa atgattgcaa tgcttaatac gatccaccca aatacaaact aacgaactat
     5701 tattcagggg tatctttatt cttttttagg aagtggggaa taaagtttga ggtcatttcc
     5761 cctacttttt tagagatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnngat aagaataaaa agtcgtgata gacatactga tcaatattgg
     5941 gcgcgatata gagcggtggg gcaattaaac aattatttaa aaactctaaa ttggtaaatg
     6001 ctgtagcggg agagaaattt ttatccactc tacttggtgc tactaagatc agttgtttgt
     6061 tatttgaaaa cttggaaagg tagagctggg catattcaag gataaatatt tcggtactag
     6121 ccatctcgaa tttagaagtc gagctgggca ccaagatgat gtcaaaatct aagaggaact
     6181 cataaggaat tgcgtgcgtc caagtaagat cggagatgca aatatcagag ttaaccgaga
     6241 aattccgcaa catagcgcgg gcatctaaaa gggttaaacc actatttttg tactccaggt
     6301 tagcttgatg aatctcaatg ccgatattcg gggctttgct agcccagcgt gaggccgagg
     6361 attgtttatc aaagtcaatt aatgagactt tcttgctctc ctggatcgct aaatacgcag
     6421 ccaaattggc agaaatcgtg cttttgccga ccccaccttt ttgattggta atcagtacct
     6481 taattgtgtc catggctaac aatccagttg taattcatag ctcaagagct aatactatat
     6541 aaataattga tttaaaacaa gtatttctgg taaatcgatg aatctagtct gagtagagtg
     6601 agctttcttc gttaaaacat acggctattt acccctttat agttaggcat tggggataat
     6661 gagtggtaat taaagcccca aaaagatacc cttggagccc atttggccga aaatcagaac
     6721 gattcgcaag accgagaact agaaccgacc gagcgacgaa ttcagcgcgc acgagagcag
     6781 ggccaactcc cgcagtctag ggacattact acgtttgcct tgttattggg ctttattatt
     6841 tttctcttaa cggcgggccc ctatttgatg cgccaactgg tcatcatgat gcaatctggc
     6901 ctgcagtttt ctaaaccgat taccttaatt gatcatattg cagagtggac ctccggggca
     6961 tttctgatcg ttctcttcat ttgcggtgta gttctattga tcatttggct gatatcaatc
     7021 tttgcaccat tatttttagt gaacttcaag gcatattttg ctttgcaatt taatttagag
     7081 cgcttagatg tatttgcagg ttttgccaga atgttctcgg tcacagtcct gttagaagtg
     7141 gttaaaaata tctttaagac tattttgatc ttgggcatta gctttgccta tctttatggg
     7201 ctatttacct tcctgcgatc gattgtcgat cttgatttca atctcgcact agaaaacact
     7261 acggtattta tcttgaacgg ttttgctttg ttaatggcac cactcttggc gattgcggtc
     7321 ggcgatgcct ttttgcaatt atttaacttt aataagcaaa tcaaaatgag tcctgaagag
     7381 atgaaacagg aattaaaaga gtctgagggc tctccagagc ttaaggcacg tatgcgccaa
     7441 cggcagcgac agattgcgtc atcgcgcatg atgtcagcca ttgaaagagc agacgtggta
     7501 ttggcgaatc cggaacacta tgccgttgct attcgctatg atcctgacag gatgtctgcc
     7561 ccaattatct tggccaaggg ggcagatgcg ctagcccttc ggattcggga agtagctaca
     7621 gagcacaatg taccgattgc tcaaatacca cccttagccc gatacctctt taatcaattg
     7681 gatattggag aatccattcc tgccccttta tttgaggcga ttgcgatggt cctggcgtgg
     7741 gcttacgaag ttaaagatgt tggttttaat aaagaactgc ccgatatcca atttacccct
     7801 gagctattaa agcctgggcg agcgcttctt tagttctgaa cacttttcgc aaaggtggcg
     7861 ccaacatctt cggggaagta ataaattaac atgagttcat tgcggttgtt gagactgcga
     7921 ccaatctgaa aggcaacttg acccttgcca aataaatagg tttttgtctt ccgtaaacct
     7981 gctttaaatg agctttcttg ccccgcagta ggctcaaaat caagcccata tttaagggta
     8041 aagcgatcaa taatctgctg ggcttgttct ggattgaaat agcccaattc ctttttaatc
     8101 acaatgagcc tgtcagactc gttaaacata atccaaacat gatcttcttt gtcatccacc
     8161 tttatcttgc aactcagagt ggattcatat tcggtgcact tgaattggtt cttaatttgc
     8221 tctttagatc cggtatagct aataccgtgg tatgccacaa ggggaagctg ctcttctttt
     8281 gcgtcaggca accaatacgc ctgcttgctc ttctcggtgt catttttact gcacccattg
     8341 agaagcgttg cagcaattag gatgggcatg acccatacca ttcgattaac agcttttttt
     8401 ctaatcatgg atgaatttta ggctatccca tcatttcttc agatttccta tttaaaaaca
     8461 agacatgatt agtatttacc ctagtctgga gccatttcgt aataaatggg accttacttt
     8521 aggcttagaa tgaaactcaa aataacactg cggagactct catgtttaat gtccaagcta
     8581 agcatccatc taccccgacc aacttggtat tcaacaatat catcggctca ggatccctca
     8641 ttcagggcga tcagactgtc aaaggacacc tgctaatttc tggggagtgt attggtaata
     8701 taaaactcga tcccctatcg gaagataccg ctgtgatcga tgcctccggt attttgcggg
     8761 gggatattgt ctgtaaaaac gcgatcgtga tcggccgggt ggatggtaat ttgagagtcg
     8821 aaaatcgtct tgagatttat ccaacagccg ttgtcaacgg ggatattgat tatgggcaaa
     8881 tctttattca tgcagatgct agagtaaacg ggcagttgca ttgtcttttg ganntgaagg
     8941 cagtttaggc ccttcatact aaattttgtt tgagagcagt ataaaaaaat ctgacatgcc
     9001 tttgcgattt ataccttggc tgctaagtat tttgatcgca gtggtgagcg gtctttactg
     9061 ccttatgggc cctggcggcc tccatgtttg gttactcttc gccttatttc tattcttttc
     9121 ctggattggg tttcgggatc tgatgcaaaa tcagcacgcc gttttgcata attacccatt
     9181 gacagggcat ctacggtttt tattcgagtt catacgccca gaaatccgtc agtatttttt
     9241 agaatccgat aacgaagaga ttccgttttc tagggcgcaa agagcgttgg tctaccagcg
     9301 cgccaagggc gcatcagaca agcgaccttt tggaaccctc actaatatgt atcagcctaa
     9361 tgtagaggtg cttctccaaa aaggcaatcc tacttctgca ccggatctga attccttaag
     9421 ggttagcgtt ggcggtagtc gctgtaccca gccttatgcg atttcattat tcaatatctc
     9481 tgcaatgagt tttggcgctt tgtcagcaaa tgccatcatg gccttaaacc gaggcgctca
     9541 aaaaggtggg tttgcacacg ataccggcga gggatctgta tcgagttatc accgcaagta
     9601 cgggggtgat ttgatctggg aaattggctc aggttacttt ggttgtcgta cgcaggatgg
     9661 acgctttgat cctgagcgtt tcaaaacagt agcgctagag cctcaaatca aaatgattga
     9721 aatcaagtta tctcaaggtg caaaacctgg ccatgggggc gtgttaccgg cgtctaaagt
     9781 aaccccagag atagcgttaa cacgtggggt tcctttnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcga atggaaaacc
     9901 agttggcttt aagctttgca tcggtaatat tgccgattgg tttgctattg tgaaggcaat
     9961 gttggagagt aagatatatc ccgactttat tgtggttgac ggtagtgagg gcggaacggg
    10021 agcagcccca gtagagtttg ttgacaatat gggaatgcca atgcgcgatg gcttgcgttt
    10081 ggttcacagc tcccttgttg gtgtaggctt gcgtgatcag attcggattg gagttgctgg
    10141 caaaattgtg agcgcttttg acatcatgcg agctctcgtg attggtgcgg attggtgtaa
    10201 ttcagctcga ggcttcatgt ttgctattgg ttgtattcaa tctaggtcat gccatactga
    10261 tcattgtcct actggggtag ccacacagga tcgcttacgg cagggcgcct taaatccgat
    10321 ggataagagc gaacgtgttt atcgcttcca ccatgagacc ttgcttgcct ttgccgatat
    10381 gtgcgcagcg gctgggataa agacacatca ggagatcagc gaagatttaa ttctgcgacg
    10441 caatcaacaa ggcaaattac agggattggt agaccagcta ataaaaattc cgccgggatc
    10501 acttctatcc gatcaggctg agcacattct tgatgagcaa atgcttggat tgggaaagca
    10561 atggcatgag gctcagccta atgtttggta ataatgaagc atcattctgc taattgaatt
    10621 accatctcca ataaccatgg ctaagaaaaa atcttccgca attcgttttt tagatcctac
    10681 cgtagagtca tttgagacaa tcgttggccc aagaacggta tttcggggaa atattgctgc
    10741 ttttgaaagt attcgaattg acggcaaggt aattggcaat atcgaatctc cgccgggtag
    10801 caatattaca gtcgccttag gaaaaacggc cacggtagaa ggcgatatca atacctatag
    10861 ggcacttatt gctggcaggg tgaacggcac tatttatgcc tccgaacggg cagagctcca
    10921 tgatggcgca gacattcatg gagaagttat ttacggtcaa attggagtgg agcaaggcgc
    10981 taaattgacc ggcacgatgt ctaggattga tccagacgaa ccacaagaag tatcccctga
    11041 ggcaatggag gtcttcgaag catttgctcg cgaagatgag gaaaacgagg aaaaaaaagg
    11101 gaagtaatcg aacctaaatt actggcttgc aacagcagta gtgggcttag gattggttgg
    11161 caaattggct tgccttggca tcaacagaac ttgggcagga tttaaatgac tgccattgcg
    11221 aataatctca taatgaagat gcggtccggt tgatcgaccg gtggacccaa cctctgcaat
    11281 taattgccct ttagaaactt gctcaccagg tttaacaaat accttttggg catgcgcgta
    11341 tttactgaca aagccatcac tatggctaat ctcaacatat ttgccataaa tcgagtcggt
    11401 agacaccttg ctaatggtgc cgtaaccgct tgcaacgatc cccgttcctg gacttgcctg
    11461 aaaatcaata cctgaatgat gcgctaagtt tttggtaaag gggtcaatac gcatcccata
    11521 attactgctc attgagccaa attgtgatgc cactggaata ccgattggaa gttgattgag
    11581 ccagtcgtat tgcgaagacc aatcattctt aacttttgtg aggattaaat ttaattcttt
    11641 tgagcttagg acggtctcat gaaagttttt atcaactgat ttgtttggat caattttgaa
    11701 tacaaatggt ttgaaaggac cgccacgggc acccccagta ttctcagtta atttactttt
    11761 cactggggca ggggtggcaa catctgcaaa gcgatctttg agagtcttta gttctgagat
    11821 tttttcaatg gacttggtga gttgagcctg aagtctctct aactcctctt tataagcaac
    11881 atctagggca tcaagctctt cgaaggtaac aacaccaccc attgctcggg cgagatctgg
    11941 tcggacttga agcgccatct taaatccaat gaaatgaatt ccgataccgc aaagaacaaa
    12001 aaaggtacca atggcaaggc caatcttcat caagcccttg ggggtgatgt tgaatttttg
    12061 aaccttagag gtggccccgg aaacccaaat cagctgcatg aattaagcct tttgttggaa
    12121 tgagccatat tttatatgaa aaacccccat gaaattgtca aaaaatgagt aatattggcg
    12181 ctaagtcatt gaaaattaat tagaaaagcc ccctttatcc ttaatgaaca tattgatatc
    12241 atttggtagg aataagagct aatttgctaa aaatgacaaa tccaaacgaa atccctgaaa
    12301 tcaaaggcgc tgaatttgca cgagcaagag agcgggtggg catgtcgacc gctgatttgg
    12361 caaagatggc ctcactgacc acaagtcatg tcgagcaaat tgagaatggt gagtcaagct
    12421 gtttttattc gcccgccatt aagtttgtag cagccaagaa gctcgcaaca tttttgggtt
    12481 tatcagaagc cgagtgtttt gtatatccag agggtgcgac agcaaagacc tcaaaatcgc
    12541 aagctgtaga gggtgctgag gaagcaatcc ataaagtaca cgtcagtatt gcaaaagaaa
    12601 attttgataa gttattgctt gccacacaag agctcgataa taaaaaacct aagttttata
    12661 aaactctttc tttttatttc ttggcgatcg ttttcttggg cggattattg ttctccctgt
    12721 tttcacgtgc cgatattgtt ggtgttatgc ctgcattcgg agagcgactc gtgattacct
    12781 ccgagacttg caagtgggat aacggcagag aagagcctaa ttggctattt gcttattttg
    12841 agacctcatt tggagtggtc aatcgagctt gttatatacg ctataacaac caaatttggc
    12901 tcagaagagc atcgggagtc gagatggtct ttcccgcgga gtattttaag caacctaatg
    12961 caagtgcaga aacagtaaaa aattaattct ttttgcaaaa aaacttaaag agtaattcac
    13021 tcatcgtatc gggaatgacc cgctcccagt tagagtcact agtatcaacg ttttgaactg
    13081 agactccacc accattgcta cctgagtagt aaccgatata aaactgctta tagctttcgt
    13141 aaccaggtcg gcaaaaatac gagatatctt ggatcacaga atagattgac tcgccgttgg
    13201 gagcgctcat tctttcttca taactaatca tttgccaagc gctcacttgt tcggccccag
    13261 gatttagaac caccttagta tcttcgtaaa aataagaatt cccttgccca tctttggaaa
    13321 tcaaaaccca cttagcatag gcgggttgca ccaatagaaa gaaaagtagg cttagcagaa
    13381 ctttattcac gatatgctac taatggttaa aattgagata atcattttac gttaagtgat
    13441 cagatgatct actttccatg aaaaaataga taaatacaaa ttgccgagag tttatatgaa
    13501 catattttgg agtctgctgg gtgtcttttc aactattggt gggatcattg cctacgtgac
    13561 cacccaaaat gttatttact ttgccatcgg gtttcttgtt ctgttttgcc aagcgtggtt
    13621 tcgttctaac gatgatgaga tcaaaaagta ttactaaatc gttttggtca ttgcctgatg
    13681 cgcgatacca gcttccctaa aaattggacc gcttgctgta aaaccaaagt tttcataaaa
    13741 cgcaatggcc tccatctggg catgtagata aaactgccgg attccctgtt ggcgaccaaa
    13801 gtctatgagg gcatttaata gagagctggc agcaccttgc cgacggcatg tgcgggttac
    13861 tgccatccgg ccaatctttc catctttttg taagcgtgcg gtaccaattg cttgcccgtc
    13921 gagttttgca atagcatgcc aggcaagtgg gtcctcctca tcaagctcca attcttctgg
    13981 aacgccttgc tctttgacga agaccgcaag tcgaatgggg taagcctgag attgtgcttg
    14041 cgcccaaggc agaatgttga cagagatagt agggcgcccc atagaagtcg atcataaact
    14101 gctatcgtta caccctatga tccgtttgac cgaacttcgc ctgccgattg accatgcccc
    14161 tgaggacctt gaggccgcaa tttgtaaaaa attagcaatt cttgcaaagg atctgattcg
    14221 gtatgaggtc tttaagcgaa gctatgatgc gcgcaagaac aatatcctta gctttattta
    14281 caccttggat ctttcggtca aagatgaaga agctgtatta aagcgtttgg cacacgatcc
    14341 ccacattcgc ctatcacccg atacgcgcta tcactttgtt gcccattttg attcccatat
    14401 caaaccgcaa agtgcactac gacctgtggt aattggtttt ggcccctgcg gaatctttgc
    14461 tgcgttgacc ttggcgcaaa tgggactcaa gccgattgtt ttggaacgcg gcaagcccgt
    14521 gcgcgagcgc acccaagata cctggggcct gtggcgcaag aagattctga acccagagtc
    14581 caatgtgcaa tttggtgagg gtggagcggg gaccttctcc gacggcaaat tatggagtca
    14641 ggtgaaagac ccaaagtttc atggtcgcaa agtcttacag gagtttgtta aagcaggcgc
    14701 ccccgaagag attctgtatg tttctaaacc gcatattggt acctttcgtt tagtgggggt
    14761 tgtcgaaaaa atgcgcaagg agattattga gttaggtggt gaagtacgct ttggccaaaa
    14821 ggtcacgggc tttgagattt ccaatcatgt acttcaaggc attcatttgg agagcggcga
    14881 ttttttagag gccgatcatg tggtacttgc cttaggccat agtgcccggg atacctttgc
    14941 aaccttgcat gatgccggtg tgtatatgga agcgaagccc ttttcagtag gctttcggat
    15001 tgagcatcca cagtctttaa ttgatcgcac ccgtttaggg ccgcatgcgg gtaaccctct
    15061 cattggggcg gctgattaca agctagtgca tcacgccaaa aatgggcgtt cggtctatag
    15121 tttttgcatg tgccccggag gaacggtggt agctgccacc tcggagtcta atcgcgtggt
    15181 gaccaatggc atgagccaat actcccgcaa cgagcgcaat gccaatgccg gaattgtggt
    15241 gggaattacg ccagacgact tccccggagg accgcttgct gggatcgagt ttcaacggca
    15301 gatcgaatcc aaagcattca cattaggtgg ctcaaattac gaggcgcctg gacagctcgt
    15361 cggtgatttt ttacaaggca agacctcgac cgagtttggc tcagtaatac cctcctataa
    15421 acccggtgtg catcttaccg atttggcaga gagtttgcct gcctatgcca ttgaggcgat
    15481 acgtgaggcc atccctgcct ttgagaagaa aatcaaagga ttctcaatgc acgatgcggt
    15541 cttaacgggg gtggaaaccc gcacctcatc acccttgcaa attaaacgtg gaccaaatta
    15601 ccaaagtatt aatacgcaag gactgtaccc agcaggcgag ggtgcaggct atgctggcgg
    15661 cattatgtct gctgggattg atggtattaa agtggctgag gctattgctt tagatttgct
    15721 agccacccag catcaagatt agatctcaat atcatccaga gcactctcgc tcaggtggct
    15781 agtatccgcc cagcattcaa cgctccaggt agaaccatcg tgtgtaatcc aattgagtga
    15841 ggtattgggt accacggcaa cgcgctcagc atctaaagct tgctgggtga cgatgcggta
    15901 gatcatatcg agtgagccgc cgtgactcac tgctaagact gattgcccac gatgctcatt
    15961 caaaaataat tcaagaatgt cttgcatccg ttgatgaaaa gtacgaatgc tctcgccgcc
    16021 ccctaaatca tgatcgagct cacgagcaat atgcctttgc caaatctctg gcagcaaaac
    16081 aggggcctct gctaatagga gtccttgcaa tttgccgaca ttgcgctctc gcagtcgtgg
    16141 agtaatttgt acatccaaag atagtgctct agccacagca ttggctgtat gcaaggctct
    16201 ttctaaatca ctcgagtaaa tgacatcaaa ggagtgctta actcgtataa gggcgttagc
    16261 aagacgctcg gcctgagaaa taccgttggc attgagtgga atatcaatat gaccttgcat
    16321 ccgccgctct gcgttccagt tagtctcacc atgacggaca aaacaaatgc gcgtattaga
    16381 catcgttaca tctctgtctg ataaatcagg cccgcatgct cacgcagcgc atgaaattca
    16441 atcatttccc agcgctgctt ggcaacatcg agctcagatt tatgtgccgc cagaaatacc
    16501 gatgcaccca ccacatcctc agccattcgg tgactgttct cggtaataaa cttttttagg
    16561 gcaactggat catcggagct cacccagcgc gccatgttgt aacgcgcggg tagtagtcgc
    16621 acctctgcac catattcagt ttgtagtcga tgggcaacta cctcgaattg caattgccca
    16681 aaagccccta agagcatcgt tccacctgta agcggcctga agacctgaat agcaccttct
    16741 tcaccaagtt gtaaaagacc tgtacgtaat tgtttattgc gcatggggtc agcggcctcg
    16801 accatgcgaa agatctcggg agcaaaaaag ggtagaccgg taaattgcaa agactcacct
    16861 tcggtcagag tgtcacctaa atgcaagagt ccatgattgg gtaagccaat aatgtcgcca
    16921 gggaaggcct catccaaaat atcacgccgc tgtgatagaa aggaaagggc attattggtg
    16981 cggatctctt taccattgcg cacaatcttt aacttcatac cgcgctgaaa atgacctgaa
    17041 caaatccgca agaaggctac acgatcacgg tgcgccggat ccatattggc ttggatctta
    17101 aagactacgg ctgagcattt tttctcatcg ggcttgacct cacgctgaat cgcatggcgc
    17161 gatccaggcg cgggggcaag gtcaaccaaa gtatttaaga tctcacgcac cccaaagtta
    17221 ttaatggctg agccaaaaaa tactggcgat tgacgacccg ccaagaactc attccgatca
    17281 aaggcgggca tggcgttttt aactaaatct acttcggcaa gagcctcttc aaaagcgctc
    17341 ccaaaacgtt ctttaaggga tgggtcatcc aaagcaagga ccgtattgga gtcttcggtc
    17401 acacgatctt cacctgcctt aaagaggcgc atctgatgat tgacgatatc aatcacaccc
    17461 gcaaaggatt tacccatgcc cactggccag gtaaaaggta ctaccgacat ccctagtgca
    17521 gtttcaatct catccatcaa atcaagaggt cttttaacct cacgatccat cttgttaata
    17581 aaggtgacta gtggcgtatg ccgagcacga catacatcta ttaagcgacg ggtttgtggc
    17641 tccacaccat tggccgcatc gatcaccatt aaggcggaat caaccgccgt taacacccga
    17701 taagtgtcct ctgaaaaatc ttggtgacca ggggtatcca gcaagttaat aatcgcatcg
    17761 cgatactcca tctgcatgac agagctnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgctt
    17821 ttcaatctcc atccaatccg aggttgcgtg acggctggct ttacgagcct ttacactccc
    17881 cgcaatctga atggcacctg catagaggag aagcttctcc gtgagcgtag ttttacctgc
    17941 atcagggtgc gagatgatgg caaaactacg acgccgggcg acttcggcaa ggaccgaatc
    18001 atttggggat gggttggaag aattattgat aaacaaccta cggaatgaat ggacaagaca
    18061 gcaaaaaaga atcgctcatt atacggcaaa gtgccgtata atttacgaat gccaagcacc
    18121 cgcactcttg cttctatacc cgctccatcc gttgctcgcc tcgaggcgcg cattagcaat
    18181 gatttgcatg ccctactaaa gcgtgccgca gagctacaag gacgcaccat gactgacttt
    18241 attgtttctg ctgtccaaga ggctgccgtt gcagcgatag agcagtcaga aattattcgc
    18301 ctaaaccaag agaaccaaac acgttttgtg aatgctttga tggagcccgc caagccaaat
    18361 gcagcattaa agaaagcatt tgaccgacgc caaaaattac taaagtccag cgcgttttaa
    18421 cactgtgtcg ctgaccattg ttccatttga tgcggcgatc agccgtgagc agtttcaaag
    18481 tggctccatg ccccttgatt gctacattcg ggagcaagct tcgcaagata ttcggcgaag
    18541 ggtgagtgcc tgctttatgg cactcaattc ccaacggcaa ctattggctt attacaccct
    18601 tgcgtcagca tcggttttgt tagatcaact acccagtact attcaaaaga aattgccccg
    18661 ctatgcggcg gtgcctgcag ttcgtatggg caggctagcg gtagataaaa gccatcgtca
    18721 gaaagggctt gggagtgcct tgcttgctga tgctctcact cgtgcctgcc gttcggaaat
    18781 taccgcattt gccttgattg ttgacgcaaa agatcatgag gctatccagt tttatcggca
    18841 ccatggtttt atgagcctct cgcatcaggg gcaaagctta tttctcccct tggctagcgc
    18901 ctctggcttg gttcattcaa agagagtggg ttcatagtgg cactcatcac actcaccgag
    18961 ggcaagttag cctttgggca tgtcccctta ctcgatcgca cagccttttc cctagaaagt
    19021 ggagagcgca ttggcctaat tggacgcaat ggcacaggga agtcatcatt gctaaagatc
    19081 ctcgctggcc ttgagaagct cgatgatggc ctcttgcagt atcaacagaa tctnnnnnnn
    19141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctc
    19201 aaggcggctg gaactgggag caacgcgtta gcgaaagctt agaccgttta cacctcgatg
    19261 ctaatcaaac gattggacag ctttctgggg gtactaaaaa acgggttgcg cttgcgcagg
    19321 ccctagtcac catgcctgat gtcttgctcc tcgatgaacc aactaaccat cttgatttgg
    19381 attcgattca gtggctagag gaactcttaa acgcctatac gggtgcaatt atcttcatta
    19441 cccatgatcg ggccttctta gatcatgttg ctacgcgtat tgtcgagctc gatcgcggga
    19501 tattacgcag ctaccctgga aattactcca gttacgagaa ggtaaaagaa caagaactca
    19561 atgcagagtc tttagcaaat gcccgagccg ataaattact agcacaagaa gaagtttggg
    19621 tgcgtaaggg tgtggaagct cggcgaacac ggagcgtagg acgtatagct cgacttgaga
    19681 agctacgtac tctaagagca caacgccgca atgcgatggg ccagatcaag cttgccatct
    19741 catccgggga tcgtagcggc aagatcgtgg ctgacttaca aaacgtcagt aaatcctatc
    19801 aaaagccgat tgtgcaaaac tttacagcaa ccattttgcg aggggataag gttgggctct
    19861 tgggtcctaa tggtgctggc aagacaacct tactcaaact gattttagga gtcatccagc
    19921 ccgatagcgg aattgcaacc atgggaacgc ggattgaggt cgcttacttt gatcagatgc
    19981 gcgaaggttt aaatctagag gctactcttg aggactttat tagtccgggt agcgagtgga
    20041 ttgagattaa tggcaatcgt aagcacgtga agagctattt aggcgacttc ttatttgcgc
    20101 ccgagcgaac caactcgccg attagtacct tatcgggcgg ggagcgcaac cgccttttgc
    20161 ttgccagact ctttgctcga ccagccaatg tcttnnnnnn nnnnnnnnnn nnnnnnnnnn
    20221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga taacgttgtt actagcatga ttgcctatga
    20341 gggcgatggt aagtggcaag agtacgaggg tggctatgag gactacagga tccaaaagaa
    20401 acgctttgat gcgttaacac aggcaaacac ggcaagtacc gtcaataaaa cggctagcaa
    20461 agaagacaaa aagtcagaga gcaagtcaga gagcaggtca gaagtaaaac aaagcgtcac
    20521 gaagagtaag ttgagcaata aagagaaagc agaattagat aagctgccta atcagattga
    20581 gcaattagaa aaaaagcaat tggtcttaag tgaacaattg gcaaacccgg agatctacaa
    20641 aggggatgca caccttatcg ttacccttaa aaatgagctg actcaaatag aagaccaaat
    20701 ggcacgctcg atgaaacggt gggaagagtt gttggggaaa gatagccttt cggattaata
    20761 gaatggcctc tgtattatta ataccatgac attctcattt cagtctcgaa ttggccttgg
    20821 aacctggcaa atgggtgagg ttgctcggtc tgaagctgag gaaattaaag cgattgaagc
    20881 tgcaattcaa atggggtatc ggttaattga tactgccgag atgtatgcct cgggcaatgc
    20941 agagagttta attggcaagg cattaaaaat tgctggacac agtaaacgcg atcaactgca
    21001 aatcgtgagt aaggtattgc ccagtaatgc cagtaccaaa ggtaccgttg cagcctgtga
    21061 agcctcgctt aagcgaatgg gctgcgatta catcgattgt tatttactgc attggcaagg
    21121 gtcgcattcg tatgaggcaa ccattgaagg tttcttgaag ctacatgagc ggggtttaat
    21181 tcgaagttac ggtattagca actttgacac cttggatttg caaaagtgga ttgctgcgga
    21241 gaatcggatc ggggcgcatg agcgtgctgt ttgtaaccaa gtctattacg ctctgaatgc
    21301 aagaggaatt gaatacgatt taattccgct gatgcaaaag caaacaatgc agttaatggc
    21361 ttattcgccc ttgggaactg gtgatttggt taatcatcct gatctaaagc ttatcgcgaa
    21421 caccctagga attacgccag ctcaactagc cttagcctgg atnnnnnctc gaagaaaatt
    21481 tagttgccag ccatattcag ctagatttaa aaactctaga gagcttggac aagatatttc
    21541 ttccgcccac tcaaaaaagc ccactaatgg ttatttgatt ggatttttat ccaacgcgct
    21601 gcaaagtggg cgcttcaaat actccctcaa gacgatcttc aagctcttcg ctggcctgtt
    21661 tcatggcggc acgcatttca tcatcaggtt gccagtagtt tcgattcgag gcttccatga
    21721 gacgattcgc catccgtgct gaggcagttg ggtttagttg cgccaagcgc tcgcgcatct
    21781 tgggatccaa aataaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
    21841 tcatagccat gctttagcat gccttcaaac catttaggat tgagcatccg agttctagtt
    21901 tcgagtgata cctgttccga cagcgttctg acaacacctt caccctgtgt ttgatcgcca
    21961 atgtaaaccg gaatttcttg cccacctttt gcacgtttaa ccgctttggt aattccacct
    22021 aaggtatcaa agtagttatc aatggtagtg acaccgagct ccatggactc cagattctga
    22081 taactcatgg tgacatcggc gagcacgctc ttgagtaggg cgttattgca aac
//
LOCUS       DDPQ01000134            4526 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_9619,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000134 DDPQ01000000
VERSION     DDPQ01000134.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_9619"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3858..3979
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1189 a          985 c         1092 g         1138 t
ORIGIN      
        1 agatctgcat cctagccgag ttcattttat ggaaactaaa cgagctgaat tcattgcaga
       61 tgtcgatact ctagaggatc ttcaaaaaga gcgtctagag tattaaagag ctttatttat
      121 ttctatgctt taatcgtatt gggagagagt ctatcaatgc gattatttag ctatcaagat
      181 aaaaaaggtc ggcataccat tggtgtaatc agcgaccaga acgcaagtag ttttattgat
      241 ctttgcgcca cggactctag gattcctaat cgtctgctgg agcttattga agcggatcct
      301 cagtttttaa tggtgaaaga taccttagcc aaccctcagg ctgtgcgagg ggagctaagt
      361 gaaatcacct tcactacctt gattgatcgc cccggaaaga tcatctgcat ggggcttaac
      421 tatgctgatc atgccaaaga gggcggccat gctagacccg agtaccccag tttttttatg
      481 cgaggaccta gttctttaac agcgcatcaa gcacccatta ttcgtcctcg agtatcgaca
      541 aaactagatt acgaggcaga gcttgccttt gtgatcggta agcaggctag acactgcacg
      601 cttgaaaacg ctctagagca tgtagtgggc tatagcatct ttaatgatgg tagtgtgcgc
      661 gactaccaac gtaagtccac ccaatggacc atcggtaaaa actttgacca aacgggtgct
      721 tttggtccat ggctggtgag cacagatgag ctacccctag gagcctcggg acttcagatc
      781 cagtctcgcc taaatggtca ggtcatgcaa aatgccaata cgaaggattt cttatggggt
      841 gttgctgaga ccatagtgct catcactgaa tgtatgagct tagagcctgg tgatgtggtg
      901 attactggta caccagcagg cgttggttat gccagaaccc cacccgtgtt tatgaagcca
      961 ggagatattt gcgaagtgga gatcgaagga attggtatcc tacgcaatac tattgcagac
     1021 gagagctaac tggctaaaag atcatttaac atagtacgtt aaatgaatac gtcttcaccc
     1081 aatttggagt ttgacaccgt tattgtcggg gctggaagcg ccggctgcct tttggcaaat
     1141 cgtctatccg ctaatccaaa ccaccgtgtt ctattactcg aggcaggcgg tgaggacgat
     1201 tggttttgga ttaaagtccc cgtgggatat ttattcacga ttgccaatcc cagaaccgat
     1261 tggtgtttta agaccgaagc agatccagga ctcaatcaac gatcgattca ttacgcaaga
     1321 ggcagggtca ttggtggctc atcgtctatt aatgccatga tctatatgcg ggggcaggcc
     1381 agtgattatg agcgttgggc agagatgacg ggtgatccca tatggcaatg gaacactacc
     1441 ctagagacct ataaatcatt agagagttat tacgcaggct ctaacgcttg gcatggcgat
     1501 gcgggcgaga tgcgggtaga gcgcccgcgt gtgcagtggg atattttgga tgcatggcgt
     1561 gaggcagccc acgagcaagg cataccaaag attgaggaat tcaatcgtgg caataatgaa
     1621 ggctgcgcat actttcagat gacccaacag cgcggtgtgc gttggtcaat ggcagatgct
     1681 tatctgcacc ctatccgcca tcgtcagaac cttactgtgc ttactcgagc acaggtctta
     1741 aaacttaatc tcagcacaat tgccccagag acattgaacc aagcttgcta tacccaaagt
     1801 gcctggcacg gtgcgcaaag tgaggttcgg ggcttggaac ttttgcatca aggagttcgt
     1861 aaaacggtca tcgccaagga tcaggtgatt ctgtcagctg ggtcgattgg ctcaccacat
     1921 cttttgcaag tttctggaat tggaccgcga ggccatttag atagtatcgg cgttaaaacg
     1981 caggtcgatt tacccggagt cggagagaac ctccaagacc acttacaaat ccgtaccgtt
     2041 tatcaggtgg agaactgcaa aacggtgaac acactgtata aaaactggtt cacacgggtt
     2101 gggatggggc tcgagtatct actaaagcgt acgggcccac taacgatgcc gccatcgacc
     2161 ttgggtgcat ttacgaagag cgatcccaat caagtcagcg ctaatatcga atggcatgtt
     2221 caacctctct cgctgccgaa gtttggggag cccctccatc cgtttaatgc gattacccca
     2281 agcgtttgca atctcaggcc aagtagtcga ggttggattc gggggacaac acccaatgtc
     2341 ttagacaacc ctaagattca gtgtaattat ttatcgactc ctgacgatct ctctgttgct
     2401 gttaatagtc ttaaaatcac ccgacagatt atggcaagct cagcactaga gtcttataga
     2461 ccaaaggaag tattaccagg cccaagtatt caaagtgatc acgatctaga acaagcagcc
     2521 agagacttgg gaaccaccat ctttcatccg gtgggtactt gcgtgatggg taaggtggat
     2581 caatccggta gagcagaaaa tcctaacacc gtcttggatt ctgaatgtcg ggtcagaggc
     2641 gtagctcgcc tacgcgtgat tgatgcttct gctatgccgt gtataacctc gggcaacacc
     2701 aatgccccgg tcatgctaat cgcagaaacc gtcgctagaa agattgtggc gcagagggct
     2761 tagcccttag gttgaagagc cgtatttctt ctggcactgc tcaagactca tctcaggatg
     2821 attgcgtctg cagcgctcaa cccgttgctc tggggtcatg ttcttcatgt tctgatacat
     2881 acttctacat tgctcaacac tcatctccgg atgacgtttt tggcaatgct caatcattct
     2941 ttgctcaggg cttggctcgt ttgccaatag tggcgcagaa aatggcacga agaccaatag
     3001 accaatgagg agtagggcgg atttgagggt gtagttcatc gctttccttt gtaataggaa
     3061 aagaatacgc tgagcgttta aagatgtaaa gggacccctc taaaatcctt taagatgagc
     3121 cgatgagaac agtttctttg ttaatcggtc tatggctact gggggctcag ccttcctttg
     3181 cccaagatcc aagccagtgc ggctttatct ctgaaagtaa ttaccgatca ctctgccgag
     3241 ccttagccga gaaaaactct agccaatgcg gatttatttc tgagagcaat ctgcgttcga
     3301 tgtgtcgagc aattgtggag aaggattcaa gtcagtgcgg cttcattaac aataatgatc
     3361 aacgatccat gtgccgcgca ttaaccgtca gtcgatagcg ataaggaaaa atagatgcaa
     3421 atcaataatc ttgttttaat cactggagca agccaaggca ttggcagggc aattgccact
     3481 cgagtcatcc aagatggata tcgggtgatt aatctcgata aaaaggcgcc taagagctta
     3541 ttagaggggg agacctatca ttccgttgat ctaatggacc ctcaggcgat tgaagagctt
     3601 ataccgaaga taacaaagca agaaccaatt ctgcgcttag tgaacaatgc tggatttatt
     3661 agacctggcg ctctagaaaa taccaccctc gatgactttg aagcagtgat ggcgcttaac
     3721 ttaagagcac caatgctctt agctcaacaa ctattacccc atatgcgcca ggctaaattt
     3781 ggtcgtattg tgagtattgc gagccgagct gcactgggga aggaagagcg gacggtctat
     3841 gcagctagta aagcaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnng tggcgcatgc ggttgcatcc ttattggatg atcgtgctgg
     4021 cttcattaca gggcaaatac actttgtctg cggcggtatg accgtaggat taagtaatgc
     4081 aacctagcat aggctaatgc acactctaag aatatttagt tagtagattt ttgatgagaa
     4141 acaatacgag tctctgcgct gtcgatgaca tcttttcgat ttaccgaggc ggtaagtgaa
     4201 tttggctgtc actaatttgt catatatttc gatataatta gaaacatgaa aaacatagat
     4261 gccatcgaag tccttctggc cttgggccag gagtcccgtc ttaatatctt ccgcttgatc
     4321 gtgcaacgcg gtgaagcagg cttaacccct agtcaattga tcgagaagtt agggataccc
     4381 aatgcaacgc tgagctttca tctgaaagag ttagccaaag ctaagttact cctagttgag
     4441 cgtcagagcc gcaacctaat ttatcgcccc aatccaaagc aaattgagag ccttagtgac
     4501 tttctgatgg acaactgttg cggggg
//
LOCUS       DDPQ01000135            7867 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_12656,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000135 DDPQ01000000
VERSION     DDPQ01000135.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_12656"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    247..298
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6300..6355
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7127..7197
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1997 a         1720 c         1934 g         2037 t
ORIGIN      
        1 gtctcgcgga ttgttgcggt tgacatcact tcggctgtat caatggtgga ggcggcttaa
       61 tgccgcttga gaaatttgct tcccaatggg gcttgtcaga gcttcaacta gctcttggtt
      121 ttattggcct actttttttg gtatgggtaa ttgtttataa cattcgtaat gccaagagtc
      181 gaaaaggtga cgaagattta cgaatagaac catcctctga gcctgcagaa cccattgttg
      241 aagagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
      301 gatcttagaa gctcttaggg agtggtctga cctacaaata ccatggatgg ctgatggcct
      361 agtccccaca aattctggtg agggtgtttg gggagtccta gatgccaatc acttatatgc
      421 ggagattcaa ttagcagtgc agttagccag tcgtcgaggc cctattgggg tccttgagtt
      481 atccgatttt tgctctcggg tgcaagccct cgcagagact ttggatgctc agatcgatat
      541 gccagcagta ggtacgatgc tcgatagctc caaggagttg gatgcacttg ctgcacaaag
      601 tgatgttctc ttaggcatca atatcgtgtt tgatcagcaa gcatggtctt ggcctcattt
      661 ggattcggta ctcacccaac ggggctttaa acgaagtaat gatggcacat cacttgaata
      721 tcccattcaa ggtaagacag tatttcgtac ggcacaattg gatcatcact cacccattag
      781 ccagatcact cttttgctcg aggtcccagt cgtttctgcc gtattacaac cattcgatcg
      841 aatgctgaat gaagcggctg atattgcaga agcgttgcaa gggcgtctag tggacgataa
      901 cggcgttaat ctcaccgaag gctctgtgaa tgtgattcgg aagcagcttg atgtcctgta
      961 tgcccagtta gaaaaatctg gcattgctgc tggatcggct actgccattc gattatttag
     1021 ttaggatagc aattgagtaa aggcagatcc tcagaagcgg atcgttattg ttatttacag
     1081 acagagcttg cgcgtctgga gcatgcttat tatgtgcttg atcaaccctt ggtacccgat
     1141 gcagaatacg atcgcttgta tcgagagctt ctagacctag agcaaaacca tccagattgg
     1201 ataacagcag attctttatc acagcgcgtc ggcggtactc ctcttaaggt atttaatgag
     1261 gttaagcatg ctgtacccat gctctcatta aataatgcgt ttgaggagtc agagttagtc
     1321 agttttgatc gtcgctgccg cgagggattg gggcttgaga cggtggagta cgcttgcgaa
     1381 ctcaagtttg atggcctcgc aatctctttg cgttatgaag gtggagtatt gatccaagcg
     1441 gctactcggg gcgatggtgc aagtggcgag gatgtcacag ccaatatccg cacaattcac
     1501 tccatccctt tgcgcttgac tggaaaacaa attccggatg tgctggaagt gcgtggtgaa
     1561 gtctttatgc accgtaaaga ttttgagcgg atgaataaac atgctgcaac cctaggagaa
     1621 aaagaatttg cgaatcctag aaatgccgct gctggaagct tgcgccaact cgactcgaag
     1681 atcactgcga agcgacccct aagtttcttt gcctatggat tgggcgcact agagcctgcg
     1741 aactggcttc ctaaaagtca tagtcaacta ctcgacttat atctggaact tggtctgcca
     1801 gtttgtcatg aacgtcgtgt tgtaagttcc gtagagggac ttcagcagtt ctatcaagag
     1861 attggagcaa agcgtaatca attgccctat gaaattgatg gcgtcgttta taaggtcaat
     1921 ttgtttaaac agcaaaacac cttagggttt gtctcacgtg cgccacgctt tgcgatagca
     1981 cataaatatc ccgctcaaga agaaatcacg accgtattgg atatcaatgt ccaggttggg
     2041 cgaaccggtg ctattacccc agttgcccgt ttatcaccag tcatagtcgg cggcgttacc
     2101 gtaacgaatg ccacacttca caatgaggat gaggtccgtc gtaaggacgt acgggtaggc
     2161 gatacggttg ttgtccgtag agccggcgat gtgatccctg aagtagtttc tgtcgtactc
     2221 gatcgcagac caaagaatgc caagcccttt gagataccta gccaatgccc aatctgcgat
     2281 tctcatatcg aacgtttggt tgatgaagca gtagcaaggt gtagtggtgg tctattttgt
     2341 gctgcccaac gcaagcaagc tttactccat tttgcgcatc gtcgcgccat ggacattgag
     2401 ggtcttggtg acaaaattgt ggatcagttg gttgatctca acttagttcg aacccctgct
     2461 gatctttatc gcttaggttt ttctgctcta gctaacttag agcgaatggg tgataagtcg
     2521 gctgacaatc taattcaatc gatcacacaa tcaaagtcta caagcctgcc gcgctttatc
     2581 tttggtcttg gcattcgcca tgtaggagaa agtacagcga aagacttggc caagcacttt
     2641 ggcaatatgc atgctttgat ggatgcgcaa ctggatgatc ttctttcggt caatgatgta
     2701 ggtcctgtag tagccaattc catcattagc tttatgtcgg agagccataa ccgagaggta
     2761 attgagcaat tgctagcatc agggattgag tttcaggtag aagagcggat tgctggtgtt
     2821 gatttgagtg gcaagacctt tgtactaact ggtacattac caacgatgtc acgcgaccaa
     2881 gcaaaggctc tgcttgaagc agcgggagct aaagtagcag gttcggtatc tcaaaaaacc
     2941 tcatacgttg ttgctggaac cgatgcaggc agcaagctcg agaaagcaac tgagctgggc
     3001 atctcagttc ttgatgaaga tgctttaatg aagctcttag gccgctaaga cttctagcaa
     3061 ctctgtttca agctgaattt gtaacttagg gttcttgctc aagttggtgc catctagcaa
     3121 cataatgtct tctactcgct cacccagggt attaattcgg gcggtgtgta acgaaacacg
     3181 atgttttgct aaagccttag cgactgcata taagaggccg gagcgatctg atgcagagag
     3241 ggtgagggcg aagtattgat tgcgttcatc gggctgtaag ctaactcttg cctgaatcgg
     3301 gaagcttttg gattgacggg agagacgacc aatactaggc tccggtaggg gcgctgattt
     3361 ctgaatcgat tctgcgagct catactcaac tagctgaatt agatcgcgat agctgccacc
     3421 ttcctcgatc aaatgactcc cagagatctg gaaggtatct aaggcgtatc catgtttagt
     3481 tgtatggatt ctggcatccc aaattgagaa atgatgtcgc tcaaaatagg cgcaaatccg
     3541 agcaaacaaa tcagcttggt ctttcacata cacggcaatt tgtaagccgt cgccagcggg
     3601 agagaggcgt ccacgcacaa tcgcttcatc cgcatgaact cgattaaaga ggtggcgtgt
     3661 gagccaagca atgtcacccg cttcttgcct taaaaagaat gcaacatcta gttgctgcca
     3721 taactcattg caagcgctgt cctcaatgcc aaataatcgc agctgacgat aagcctcttc
     3781 ttgattctgg atcaactccg aggatgggtc tggtttggca ccccctagga ctcgtaaagt
     3841 agcgcgatac agatcctcta ataatttccc cttccaagca ttccaaacct taggactggt
     3901 cccccgtata tcagcaacag tgagtaggta gagggcggtc agatgacgct catctcgcat
     3961 gcgttttgca aaggcagtaa tgacttcggg atcagtaata tcttgttttt gggcaatttg
     4021 gctcatggtt aaatgttctg ccactaacca tactaagagt tcagtctcat cggcagagag
     4081 cccatgatcc tttgcaaagg cccgtgaatc tcgtttccca agctcagaat gatcgccacc
     4141 acgcccttta gcaatatcat gaaagagagc tgccaagaca atgatccaac tcttttcaaa
     4201 gctagcaatt aatttgctac aaactgggaa ctcatgggtg tgctcaataa tcatgaagcg
     4261 cctaacattg cgcaacacca tcaggatatg ctggtcaaca gtgtagacat gaaaaagatc
     4321 gtgttgcatt tgcccgacga ttcgtcgaaa tgcgggtaga tagcgaccca gcacactggt
     4381 ctggttcatt aaacgaaatg cagtggtcac gccgtctggc agtttgagta gatccatgaa
     4441 tgcgacacgg tttctgggat ccttgcgcca tgaggcatcc attaattggc gagcgttgta
     4501 tagagatcta aatacagtcg cggataggcc attgatagct ggatttttcg caaagaccaa
     4561 aaaggtttgc aaaatcgctt caggatgttt ctgatagagg tctgaatcca caatatccaa
     4621 taagccctgc ctctcgataa acatctcgtt accaagtccg gggatggggt gtgtcgtacg
     4681 cgcttctttt ggaaacagta aggcctcaat gttttgtacc aaaatggtat tgagctggct
     4741 aacagccttc gccgcccaat aatattgccg catcagttcc tcgctggcta gacgagtgga
     4801 ctcttgttga atacccaatt gtttggcaag ggccgcctgt aaatcaaata ccaagacatc
     4861 ttgccgccgt tttgctaaga gatgcaggtt ggcccgaatc gtttgcaaga gtttttgatt
     4921 gcgattaagc tctttgagtt ctcgggcggt aatcaaccct tttttatgta ggtccgcaaa
     4981 actattacca agcccagccg ctttactcac ccacaagata atttgcaaat cacgcaaacc
     5041 gcctgggctc tctttgcaat tgggttctag tgagtatggg gtctcttgat acttgtagtg
     5101 tcgttgctga agctctaaga gctttgcctg aaaaaaggct ttgggatcca tggcctcatc
     5161 taaattgcgc ttgaagtgtt tgaagagacc acggttaccg ccaatcaggc gcgcttccaa
     5221 aacagaggtg cgaaccgtaa tgtcaccagc agcttcggat aagcactcgg atagggatcg
     5281 aaccgccgaa ccgatttcta agccggagtc ccagcaacga gcaataaatt tctcaatatt
     5341 gctagacaca tgttgatcgt gtgaattcgt ttgatcgtct agtaagacca aaatatcaat
     5401 atcagagtgg ggaaagagct ctccacgccc atagccaccc acggctagta aggctgcatc
     5461 gtgatgaaga ccagactcat tccacaggtg aaggagtaat gcatcggttg cttgactaag
     5521 cttttgcatc agcttcgata cattctgatt ctgcaagaag tcagagcaag ctaaagctct
     5581 tgcatcgttg agtggattgg cccctgaacg cattactaaa aattgtctgg cgatggtaag
     5641 gcttatgctg cgactgggtt aggacgatat tgcaatggac caacacagtc aggcgccttg
     5701 ggggtgccgt ttgaccaggt taaaacctcc acgccagtat gggtcactaa aacagtgtgc
     5761 tcccattgcg cggacaaact acggtctttc gttttgacgg tccactgatc gggcatggtg
     5821 cggatctcac gtcgaccggc attaatcata ggctcaatcg tgaatgtcat accctctaca
     5881 agagtttctc cagtgccggg tttgccgtaa tgcaggattt gaggatcttg atgaaagact
     5941 ttgccaatac catgaccaca atactcccga actactgagt agcccgcatt ttctgcatgc
     6001 gattgaatcg cgtagccaat gtcaccaagt cgggtgcctg gcttaacttg tgcaattccg
     6061 agccacatac attcaaaggt aatttggttt aagcgagcgg ccatgatcga aatctcgcca
     6121 acattgaaca tgcgactggt atcgccgtag taaccagcct gcgtaatcac agtaatgtct
     6181 aaattaacca catccccatt tttgagaacc ttatcgcctg gaatgccatg gcaaataaca
     6241 tcattcaccg aggtgcaaat ggcggcagga aaagggggat accccggagg ttggtagttn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaagac
     6361 ctcgctagca aggcgaccag cttcgcgcat cccttgtagg tctttttcat gggtaaatat
     6421 gctgtgcata gctcacatta tggctgaata tcctgaatca ggcctgatct aaaacccact
     6481 agatgactga tatttaaggt ataatttgca gatcgtcctc atagtggggg cgaaatccca
     6541 ggtcaagcca tccagggtgg cgcttttgag cgcagtcagg acttgacctt agaaccaacc
     6601 cttaggagat tgtaatgtca gtgacaatgc gtgaaatgct ggaagctggg tgccattttg
     6661 gtcaccaaac tcgcttctgg tcccccaaga tggcccctta tatttttggt catcgcaata
     6721 agattcacat catcaattta gagaaaacct tgcccatgtt tcaggacgcc ctgaaatttg
     6781 tgcgtcaggt tgcctccaac cgcggcaccg ttctctttgt cggtactaag cgccagtcgc
     6841 gcgaggtaat cgctgaggaa gcagctcgtg ccggtatgcc ttatgtcgat agccgctggc
     6901 tgggcggtac cttaacgaac ttcaaaactg tgaagggttc gatcaagcgc ttaaaagata
     6961 tgaccgctgc taaagaagcg ggtgattggg agaagctttc gaagaaagag gcgcttacca
     7021 atgagcgtga tctggataaa ttacaaaaat cactgggcgg gattcaggat cttaatggtg
     7081 tgcccgatgc aatttttatc gtcgacgttg gctatcataa gattgcnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
     7201 gcagttgttt tgtatgttcg cggaattgcc gatgcaattc ttgaaggcaa ggcaaatgca
     7261 gttcaacaag ttcttgagtc tgttaaagag ggcgaagaag agtttattga agaagggaag
     7321 gtagactaat gcccgcgatt actgcagcaa tggttggcga actccgcgcc aagaccgatg
     7381 ctcccatgat ggagtgtaaa aaagcattga cagaagccga tggcgatatg accaaagcag
     7441 aagaaatttt gcgggttaag ttaggtagca aggcgagcaa ggcagcatcg cgtgtcacgg
     7501 cagagggtgt gattgtttcc tatattcagg ggaatacggg cgcactctta gaagtcaact
     7561 gcgaaactga tttcgtttca aagaatgatg acttccttgg ctttagtaat ggctgcgcta
     7621 agttggtggc tgagaaaaac cctgctgacg ttagcgctct agcgaccctg tctttagata
     7681 gtaaaacagt agaagctatt cgcgctgagt tgattggcaa gattggcgag aacatgaacc
     7741 cgcgtcgctt taagcgtttt gcgggtggcg gtcaattagt ttcctatctc cacggcaatc
     7801 gtattggtgt gatggtggaa tatgagggtg atgctactgc tgctaaagat gtggcaatgc
     7861 atatcgc
//
LOCUS       DDPQ01000136           10367 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_12654,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000136 DDPQ01000000
VERSION     DDPQ01000136.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_12654"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    162..254
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8315..8351
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9570..9636
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10110..10134
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2539 a         2634 c         2338 g         2634 t
ORIGIN      
        1 ccgacgttgt gcgcgttcaa tccaccactc aaaacgaccc gaggcgcgtt tgcgtttgcc
       61 catgatgttg gtatttgatt ccaatcaaaa gttgtaccgc ttccaccata gccctcgatc
      121 agggcatcta gcaaaaagcc ggctgcttcc ctatattgta gnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnncccgcc aagcgtttac attgctctgg ggtctcatcc ccatgaaact
      301 gccacatcgt aatagccgca ttcgccctaa tctcctcaat ctgattgggg gtcgggttca
      361 ctaccaggcc cacggcatca atccctttgg ggagttgccc aatcagggcg ccggcatctt
      421 cgggcttaat agcgcgggag ctcggagggt aaaacaccaa gccgatggca tcgacattgg
      481 ccttaactac cgcttcaata tcagcaggta tgcgtagacc gcagatcttt attctgctgg
      541 tattgggggt gtaatcgagt aatcccattg ttttgttcat ctaccttata ccgaaagcat
      601 aagctctttt ggcaaccatg cattttgcca atggggagtt ggaatggcta ggtccttggg
      661 gtagcccact tccattaaat acaaaccatc gggtgcaaag gtgggtgctg ctaactcccg
      721 attttttgct tgcaagaccg tcttgatcca ctcgggcttt tgtttgcctt ggccaacgac
      781 caagagactg cccacgatat tacgcaccat atgatgcaag aaggcattgg cgcgtatctt
      841 gaagtagagg aatggttgct cgctggtgat actgcattca tataagtgtt tcatgggtgt
      901 tttactctga cactcggatg cccgaaacga tgagaaatcc tgctcaccaa ttaaatagtc
      961 tgcagcgatt tgcatcgcac tggcatcaaa ccactggcca ctgggcatca tgtgatagcc
     1021 cacccgtttt gctagtgtgg gtgaccgggt tggacctgcg acgatggcat agatataggt
     1081 gcgctcgtgg gcggtaaagc gggcatcaaa atccgggggc actacctttg cccaattgac
     1141 aacaatatca ttgggtaaga aggtattaat ccccctcacc cacgaccatg gatcgcgctc
     1201 aacatcggca tcaaagtgca cgacctgacc aagcgcatga acaccggtat cggtacgacc
     1261 ggcagcagta actctcacca gttctttctt gccgataaat tgttgaatgg cttgctcaag
     1321 cgtatgttga accgtcgcct gatccggctg gatttgccaa ccacagtaag cactgccatc
     1381 atattgcaca cccaaggcaa tgcgcatgat attaagaact gctacgttga ttaatctcgg
     1441 acaataagct cttcgcagaa atcgtcattt gtgggtccac ttgattactc acgagcaata
     1501 tctcttcaat cgacttacga gctgcggcat agtcttcgat cgtaatatag gctttggcta
     1561 aattgagttt gacccgcagc gattcagggg ctggcgtttc tgtgggtggg cttgactcga
     1621 gattgagatc taaatccgcc agaatagcgg cagctctttg cggtatctct cccccaccag
     1681 tttgggtatt tggttcaccc attgcagttt ctgttctagc atccgatgaa gcttcaccgc
     1741 gtcttgcata acgcgccaag gcgagcaaca aaataccggt aatcgagata aataccaatc
     1801 ccatcccaat ggtcgtccac tgatcattgc ttatcttggt ccccctactg acttcagagg
     1861 aagcgctttg tttgcgagca tctaataatt tttggaggtc tgcaatattc ttttctagct
     1921 cagcaagttt tgctttggtc tgtgcaagcg cccgttcttg aactacgatc tcttcaatat
     1981 agcggcgcgt gtctgcatca gggcccgctc cagggccaat tttgaggcga tcgtcaccgc
     2041 ccttagaggt tttactagct gccccactcg gtacagatcc ctgtccttgc tctccttgtt
     2101 gcttaagcca cgtttgattc gactccagca caaactcttt ggcctcagcg gatgtgattg
     2161 aacgaagaat gctagcgtcg ggtttcgtta actcagctcc agcaatcaga cgatgaatac
     2221 tccctccaat gaatgcatcc ggatttgttt tatacagcgc aaggattgct tcgtccatgt
     2281 ccgcaccact aagttgtggg gtcatttgca aggcaatctc gctgagggtt tggccaggct
     2341 ttacgaccac cttggtgacg tcaccaacca tcagggtgta tggttttgca atggctccgg
     2401 aggcccactt caatccaata atgatgtcga tgaagggatc ttgatcgacc acgatcgccg
     2461 tatcggcatc gatcgtaata atctgttgcc ctgggcttac tttgcgtaaa gagatgcttg
     2521 gtttgtaatc caatatcttg ctagagatgc ccaagcgttc gtactccgat ttgggaacca
     2581 gatcaatact taaggtctca agaatatttt tatcatctgc tttggctttg accgggatct
     2641 cgactttgag aggctcaccg ggttttgaca acatcatcgg tgatcccagc gttactgcat
     2701 ggaccgtggt ccacataaag caactcacaa tggcgaatac gagccaatgg aatagcgttt
     2761 gcagattacg agaccgggtc atttttatct ttctagcaaa atccgtaaca tgcgacgcaa
     2821 tggctcagca gcaccccaca ataattgatc gcctactgtg aatgctccta aataatcagg
     2881 gcccatcgct aatttatgca agcgcccaat cggtatggtc atcgtaccac ttaccgcagc
     2941 aggcgagagc tctcgttctg tggtctcacg atcattgggc accactttga cccattgatt
     3001 atccgcagca agcatcgcct caatctcggg caaaggaatg tctttcttga gcttgatggt
     3061 gagaccttgc gagtggcaac gcattgcacc tacgcggaca cagatcccat caatggggat
     3121 gctgccgggc gatctaaatg gggggttacc cataatcttg ttgcactcaa ctccggcttt
     3181 ccactcttct ttggtttggc cgtgctccac cggcacatca atccacggaa ttaagctacc
     3241 agctaaggca gtattcctaa agtttgtttt tgggaactgc gccgagcgca tggtttgcgc
     3301 tactttgcga tcaatatcca aaatccagga tgcggggttg gctaaatcac tggcaacgct
     3361 ctgatgcaag ctacccattt gctcaaggag ctcacgcata ttagctgcac ctgcgcccga
     3421 tgccgcttga taggtcatgg cactaatcca ctcaaccatt ccactcttta agaggcctcc
     3481 cattgctaac atcatcaaac tgacagtgca attgccacca atccagttct taccgcccgc
     3541 ctttagcgct tgattaatca cgggtcgatt aattgggtct aacacgatca ctgcatcgtt
     3601 ctccatccgc agggtgctag ctgcatcaat ccaatgacct tgccaagcct gttgacgtaa
     3661 tgccggataa atcgctttgg tgtaatcgcc tccttgacag cttaggatca catcgcaacg
     3721 agcgagcgcc ttgagatcat tagcgtcttg caaacgggtc tcttgtttag ataccttctt
     3781 accattaata aggggtactt caccaccagc attactggtg ctaaagaaca cgggttcaat
     3841 ataagcaaag tcgtcttcgt cttgcatgcg ttgcatcaag acgctgccca ccatgccgcg
     3901 ccaaccaact aggccaacta caggatttac gagaggagtg tgtgccataa aaatgtgtac
     3961 ttatcgatta atcaatgact accctaatgc ttttacaact gcatcgccca tctgcacagt
     4021 cgatactttt tgcgtacctt cggtataaat atcagcggtt cgcaggcctt gtgacaaaac
     4081 cttctgcact gcggtctcaa tccgctccgc ttcttttgga agatttagtg agaaacgcaa
     4141 catcattgca gccgacagaa tcgtagccaa aggattagca atccctttgc cagcgatatc
     4201 gggtgcagag ccatgactag gttcatacaa gcccttattg ttcttatcta aggaggcaga
     4261 tggcaacatg ccaatggagc cagtaagcat cgccgcctca tcggacaaaa tatccccaaa
     4321 caaattaccg gtcacgatca catcaaagga ctttggtgct ttgaccaatt gcatcgcggc
     4381 gttatcgaca tacatatggc tgagctcaac atcgctgtac tctttggcaa tacgggtcat
     4441 cacctcgcgc cagagctgtg aggtctccag cacatttgat ttatcgacgc tgcatacttt
     4501 gcgatgacgc ttgcgtgccg cctcaaatgc cacatgcgca atccgctcaa tctcgggttc
     4561 gctatagcgc atggtgtcaa agccctcacg ggcaccagca aacaatccat cgggtgcatt
     4621 gcggattcct ttgggggaac caaaatagat atcgccgttg agctcgcgca caatcaagat
     4681 atccaatcca cccacaatct caggctttag gctcgatgct gcagtgagtt ctttataaca
     4741 aatggcgggt cggaagttag caaaaagagc caagtgttta cgtaatccca aaattgcttg
     4801 ctcgggtcgc agctcacgtg ccagggtgtc gtatttccag tcacccaccg caccaaacaa
     4861 aattgcatct gcggctttgg ctaaatctaa ggttgctggt ggcaaaggat ggcctgccac
     4921 atcgtatgca gccccaccga ctggtgcgga ttccatagtg agtggtaatt gaagagcttt
     4981 gagaacctta acagcctcag ccacaatttc cgggccgata ccatcgcccg ggagtactgc
     5041 gatattcata aataaccttt gttcatagtg ctgtgatgaa gcattagcgc tagcccgcca
     5101 cggctacaac aacttagggt agctgggttg caagccatgg cattcgcaat aagcgttctg
     5161 cctcaaaagt ctttatttta tcggcatgtc gtagggtcag accaatatcg tctagaccat
     5221 tgaccaggca atactttcta aagggggtta cttcaaacga atagcttcgg ttatcgggag
     5281 caatgacctc ttgacgctca agatcaatag tcagctggta cccagggaag gccttggtct
     5341 cattaaagag gtgatccact tgctgttcgg tcagaacgac gggcaatagg ccattcttaa
     5401 agcaattatt gaagaagatg tctgcaaaac tgggggcaat aatggcacga aagccaaact
     5461 gcgagagtgc ccaaggggca tgctcacgcg agctaccgca gccaaagttc ttacgagcca
     5521 agagaatacc ggcaccctga tagcgggtct ggttcagaac aaaatcaggg tttactgggc
     5581 gcttagaaca atcttgcccc ggctcaccgt gatccagata gcgtaattca tcaaagaggt
     5641 tctgcccaaa tccagtcttc ttgatcgatt tcagaaactg cttcggaata atggcatcgg
     5701 tatcgacgtt ctctcgatcc aatggggcaa ctaagccctt gtagactgta aatgcatcca
     5761 tttatttaac cggtgtgatc gttacgtctt tgttgggatt tgtgctgggt gttccagaac
     5821 tgctattggg cgcggtctct acgggctttg cttgtggatc cattttcttg ccaatttctt
     5881 gtaaatcttt cccaacaccc gtcatagtgt ttgagcaagc aactaaagat cctgcgatga
     5941 cgagagaaag aataaatggg ctagttttca tatgaaccta ttgtataaag attaggctat
     6001 cttccgaata tcgacaaagt gaccttcaat cgcagcagca gcagccattg ccggactcac
     6061 caaatgggtt cgaccacctg caccctgacg accctcgaaa ttacggtttg aggttgaagc
     6121 gcagcgctct tgaggttcca agcgatcagc gttcatagct aagcacatcg agcaacctgg
     6181 ctcgcgccac tcaaagccag ctgctttaaa gacccgatct aatccttcac gctctgcctg
     6241 cactttgaca agtccagacc caggcaccac taaggcctgc ttcacgctgc tggctacttt
     6301 cttgcctaat cgctcaacca ctttagctgc agcgcgtaga tcttcaatcc gactgttggt
     6361 gcatgagcca ataaatacct tatctacata aatagtatct aagggcatat tgggttcaag
     6421 gcccatgtat tgcaaggcac gttccatcgc ttgacgcttg ttgggatcgc gctcgcgctc
     6481 tggatcagga accctgccgc taatcggcaa gaccatctcg ggcgatgtgc cccatgtcag
     6541 ttgcggcaca atctcttcgg cccgcaactc aaccacttga tcaaagtgtg catcggcatc
     6601 agaatgcagc cctctccagt attgcaaggc aaggcgtaat gcctctcctt ttggagcgta
     6661 ggggcgtccc tgaatgtatt caatggtgat ctcatctacc gccacaagac cggcacgcgc
     6721 acccgcttca atcgccatat tgcagatggt catacgccct tccatcgata aatgacgaat
     6781 ggtctcacca gcaaactcga tggtgtaacc cgtaccacca gcggtaccaa tcttcccaat
     6841 caccgctaag acaatatctt ttgctgagga gccaggttgc aaacgccctt caactcgcac
     6901 caacatgttc ttgcttttct tcatcagcaa agtttgggtg gctagtacgt gctccacctc
     6961 cgaggtacca atcccaaatg cgagagcacc aaaagcacca tgcgtactgg tatgcgaatc
     7021 accgcacaca acggtcatac ccggtaaggt agcgccctgc tcagggccaa tgacatgcac
     7081 aataccttgg cgcagatcgt tcatcttgta ctgggtaatc ccataacgat cgcagttttg
     7141 atctaaggta tccacttgca gttttgatac gggatcggca ataccctcgc tacgatcggt
     7201 cgttggcaca ttgtgatcag agaccgctaa gttggccgag gttcgccaca cggggcgcgt
     7261 tgccaagctc aagccctcaa atgcctgtgg gctggttact tcgtgcagta gttgacggtc
     7321 aatgtataga gtcgctgtgc catcctcttc cgagtgcacc acatgctcat cccatagttt
     7381 gtcgtaaagc gtacgcattc aaagtcccta tcaacccaaa tccattaaaa gcttattttc
     7441 gctcagaaat ggctggcacc ttgcgtggcg ttgctccggc aaataattgg cgggggcgac
     7501 cgatcttgta ctcggggtca gtaatcatct cttcccattg agcaatccag cctacagttc
     7561 ttgccaaagc aaaaatacag gtgaacattt ctgtggggat gccaagagca cgttgcacga
     7621 ttcctgagta gaaatccaca ttgggataaa gcttgcgtcc cacaaagtaa tcatcttcta
     7681 aggcgatctt ctcaagggtc atggctagct tgaacagagg atcattctct aagcctaact
     7741 cttttaatac ctcatgacag gtctcgcgca ttaacttggc gcggggatcg aagtttttat
     7801 aaacgcgatg accaaaaccc atcaagcgca cattcgagtt tttatccttc acttggctaa
     7861 taaactcacc aatcttctca accccaccat ttgcctgaat cgaggtcaac atctcaaggc
     7921 aggcttcgtt agcaccaccg tgggctgggc cccagagaca ggcaatacca gcagaaattg
     7981 ctgcaaatgg attcgtgccc gaagagccgg ctaagcgaac agtcgaggtg gatgcatttt
     8041 gttcatgatc tgcatgtaag gtaaagatac gatccaaggc ctttactaat actgggttaa
     8101 ctttgtatgg ctcgcatggg gttgcaaaca tcatgcgcat aaagttagcg gtgtaagaca
     8161 aactattgtc tggatacata aagggctgtc caatcgaata cttataggac atcgccacca
     8221 aggtaggcat ctttgcaatt aagcggatct gagcaatctc acgcgccttt ggttcgctgt
     8281 aatcaatttc gtcatggtag aacgcagcca ttgcnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn naagaactgc atttgctcat gcaccatggt gtgatgcatc accagatctt
     8401 caaaatcagt cttttgctta gcgtttggga gttcgccacc aattaagagt tggcagacct
     8461 ctaagaagtc gcattttgta gcaagctctt caatggggta accccgatag agtaactcgc
     8521 ccttatcgcc atcgatgaag gtaatctttg aactgcaaga ggccgtagac aagaaccctg
     8581 ggtcataggt gaacttaccg gtctgaccat acaacttccg aatatcaata acatctggac
     8641 cttgtgtgcc cttgtaaatt ggtaactcga tatcgggagt gccgtccgag aacgagagct
     8701 tggctttgat attcgattca atcatgattc tgtcctaata tcgattaatt attaatacat
     8761 tcattgtcgc tgacgcaaca tactcaacac cgccctactt gtctctgtgt ctaggtctgg
     8821 gtttaactct ttcctagcga gcagcaagtc gagtaaatcg ttgtcatcta gctttaacaa
     8881 ctcagacaat gctgccccta aatcttcatt caactctgag gcataacgct gaaagaaacg
     8941 ttctagtatt aaatcgtttt ccaacaaacc gcggcgtgct gcactacgaa gttgatacaa
     9001 ctgtttagca tctaggctca taccgcccga cgcaccatca actctttaat cttaccaatt
     9061 gctttagtgg ggttgagatg cttagggcat acatcaacac agttcatgat ggtgtggcaa
     9121 cggaacagac gatacgggtc ttccaagtta tccaaacgct cgcctgttgc gtgatcacga
     9181 ctatcagcaa tgaagcgata ggcttgcaag agtcccgcgg gtcccacaaa tttatcgggg
     9241 ttccaccaga acgatgggca ggaggtggaa cacgaggcac acaagatgca ctcatataaa
     9301 ccatctaact cctcgcgctc agccggactt tgcaaacgct ctctctcggg tggtggggtc
     9361 tcattaatta aatacggctt gatggataga tactgcttaa agaacaaggt catatccaca
     9421 atcagatctc gcactacagg caagcctggt aatgggcgca atgtgatctt cttaggcaat
     9481 gagcgcatat tggttaagca cgctaggccg ttcttcccat tgatgttcat agcatctgaa
     9541 ccgcatacac cttcacgaca ggagcgacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgcg ttcgcctttg agctcgagct
     9661 cataggtctc catccgcggt gccttatcga catctgggtc ataacggtag atttcaaaga
     9721 tacgggtatc actcatagtg tttatccaag tattagaacg tacgctcttt gggtggtaca
     9781 gagtccaccg ttaagggttt tagttgaacg ggtttgtact ccaattgatt accttcgtac
     9841 cacagggtat gtttcatcca attagcatca tccctctttg gatactcgtg gtgggagtgc
     9901 gcaccacgac tttcggtgcg cgctgctgca gagatcatcg tggaattagc ggtctgtatt
     9961 aagttatcaa tctccaaagc ctcaatccgt gcggtattga agatctttga tttatcttta
    10021 agccaaacat gcttggcacg ctcggtgagc tcagccattt tgcgcacacc atcggcgagc
    10081 aactcttgat tgcggaacac acccgcatan nnnnnnnnnn nnnnnnnnnn nnnngagcta
    10141 cttgtgtcta gcttggcaat acgttccatc gtgtattcac cggcatcgac tggtaaaggc
    10201 ttatggtctc ttgctttgag attggcttgc accacatggt taccagcggc acgaccaaat
    10261 accaatagat ctaagagtga gttagtaccc aaacgattag cgccatgcac cgatacgcaa
    10321 gagcactcac cgattgcata aagaccttct acaaccgtgt tggggtt
//
LOCUS       DDPQ01000137            5400 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_23399,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000137 DDPQ01000000
VERSION     DDPQ01000137.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_23399"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT         1426 a         1242 c         1228 g         1504 t
ORIGIN      
        1 tttaaagagt gctaaatccc aagttaccta gatcatgaat cgctaccctc tttggaaata
       61 cctcgttatt gcggtagcgc ttctgattgg ctttttatat tcattaccca atatttttgg
      121 ggaagcccct gcggtacaaa tctcttctgc aaagccaaca attaaggttg accttgctac
      181 ccaatctcgg attgagagca tattgagcga gggcagtatt aaaaacacag gtctgttctt
      241 cgagcggact ggcaatattg gtagcatcaa gattcgattt gacaatacgg atactcaatt
      301 aaaagcgcgc gatctcatta atcaaaagct caacgttgat cctaaagagc ctaactttat
      361 tgttgctctg aacttactat ccaatacacc gcactggctt ggttccatta atgccctgcc
      421 aatgccgctt ggtcttgatt tacggggtgg tgtgtacttc ttattgcaag tagacatgca
      481 aggcgccgtc caaaagaagt tgacctcgct cgcaacagat attcgtggtc agttacgaga
      541 taaaggcttg cgccatcaag gaattgagcg aacgggtgat gtgattaata tccgctttgg
      601 ttcaactgcg gaagcagatg ttgctcgcac agccctaatt gggccccaag ctgagcttga
      661 gtggcaagta gagaagtcag cggatggcgc caaattagtg gggcgtttta aatctgcatc
      721 cctcaaagaa gttcaagaaa atgcagtaaa gcaaaatatt attacgctca ataaacgcgt
      781 gaatgagctt gccgttaaag agcctgtcat tcagcaacaa ggcgcagaac gtattgttgt
      841 ccagctgcct ggcgtgcaag atacggcaag agcgaaagat attattggcc gcaccgctac
      901 tctagagtca cgtttggcag atccagtcgc ttcgagtatc ggcttgaatg aaacccctcc
      961 cccgggaacg gatgtgttcc gctttggcga aaatcgtctg ggggtcttta agaaatcggt
     1021 aatctttaca ggcgatcgca ttactgatgc gagtgctgga tttgatcaaa accaacgccc
     1081 ctccgtgaac atttctttag atgctgccgg tggccgtgtc atgcaagaaa tcacgcgcga
     1141 gaacattggt aaaccaatgg gcatgattct gtttgaaaag ggcaaaggcg aggtattgac
     1201 cattgcaacc attcaaagcg aattcggctc caagttccaa atcaccggtc aacccactac
     1261 agagagtgct aatgatctag cccttctctt acgcgctggc tctttagcag cgccgatgga
     1321 aattattgaa gagcgcacta ttggtcctag ccttggtcaa gaaaatatcg agaagggctt
     1381 taaatccttg atatatggat ttgcagccat tgctcttttc atgaccgcct actacatgct
     1441 gtttggcctc ttctctgtga tcgccctcgc tgtaaatatc gtcttactga tctcattgct
     1501 atccatgttg caggccactc taaccttgcc agggattgca gcgatggccc ttgccattgg
     1561 tatggcgatt gactcaaacg ttcttattaa tgaacgtatt cgggaagaat tacgcaatgg
     1621 cgtagcgcct cacaccgcca tcgcaattgg ctttgataaa gcatgggcaa ccatcttgga
     1681 ttcaaatatc accaccttaa ttgctggcct tgccttactt gcgtttggct ccggtcctgt
     1741 gaaaggcttt gcagtcgttc attgtttggg tattttgacc tcgatgttct cggcagtgtt
     1801 ctttgcacga ggggttgtta atttttggta tggtcgtcaa aagaaattac aggcgatttc
     1861 aattggccaa gtctggcgcc cagaaggaaa ataaatcatg gaatttttcc ggatccgtaa
     1921 agatattccc ttcatgcgcc atgcattgct tctcaatgca ttctcgttga ttacttttat
     1981 agcggccgtt tttttcatct ggcaaaaagg cttacattta tccatcgagt tcactggtgg
     2041 aactgtgatg gagattacat atcctcaaac cgcacctcta gactctattc gtgatcagat
     2101 tacgaagata ggctatacag atacccagat ccaaaatttt ggtagctctc gggatgtaat
     2161 gattcgtctt cctttgcaaa aggatcaaac aggtcaaatt attccctctg ctgatcagag
     2221 tgcttctgta atgaaagtgc ttgaagcaaa taattctgga gcaaaactac aacgggttga
     2281 atacgtaggg ccccaggtag gcaaagagct tgcgattgac ggtttgaaag ccttagcctt
     2341 tgtcgtaatt ggcatcatga tctatttatc cttccgtttt gagtggaagt ttgcggtcgc
     2401 cggtatctta gctaacttac atgacattgt tatcattttg ggcttctttg ccttctttca
     2461 gtgggagttc tcactctctg tgctggcagc cgtgttggcg gtcttgggtt actccgtgaa
     2521 cgagtccgtg gtgatctttg accgaatccg cgagaacttt cgtaagcaac gcaaaatgga
     2581 tactaaggcg attattgata gcgcgatcac tggaacaatc agtcgaaccg ttattacgca
     2641 tggcagcaca gagatgatgg tgattgccat gctcatcttt ggcggcccta ccttgttcta
     2701 ctttgctctc gccctaacca tcggtatttt gtttggtatt tattcgtcgg tgttcgttgc
     2761 ggctgcgatt gccatgtggc ttggtgttaa acgtgaggat ctaattaagg gcgagcgcaa
     2821 agcgagcgac aataatcgcc cagatgatcc taactttgga gcgcaggtct aatccgatct
     2881 aagattcttt gagttgcgcc ttgatgctgg gaggcgtact gcaaggcttt tgcagacatc
     2941 atctgacgtt gactggtatt cgtgagcaaa gccttaagag cctcactcaa tgattgcacg
     3001 agatcatggt tggagccttc aatccgcacc gccgccccac aggtaattgc atcttcacta
     3061 gcctgctgaa agttatacgt gtgctctccc aaaatgactg gacacccatt ggcacaagct
     3121 tcaatcaaat tttgtccacc ggttggcaaa agactgcccc ccattaccac caaatcacta
     3181 gcgctgtaat aagctgccat ctcacccatt gaatctccca gcaagatatc catggaggcc
     3241 caatgcaaag atgggtcaag ctctgtactt gagcgtgggt cacttaaact agaccgtctc
     3301 tcaaatgaca aaccggattg atagagtaaa tcggccactt ctgtaaatcg gtcaggatgg
     3361 cggggaacca aaataagtaa gggcttttta agtgagtcgt gttgctgaag ataccttttc
     3421 catgactcaa taatgaggct ctcttcgcct gcacgtgtac ttgctgcgca tactgccaaa
     3481 cgcccttgat tacttagggc acgcttccat tgctttccta aagactgaat agaggtctgc
     3541 tcatgtacat caaatttcat attaccggta acgatgacat ctttgacacc gagtgcttgg
     3601 taacgttttg catctggctc agattgtgcc aagatgcctg caaaggattg gaagagggat
     3661 ctgcctgcag aaccaaagtg ctgaacgcgc ttaaaactac gatttgataa acgggcattg
     3721 atcaaaaata aggggatgcc aagttgcttg gtccgaaata caaaacctgg ccacgcctca
     3781 gtttccataa aaagccctat ttggggacga aagtatttta ggaaagcttc aattgcccag
     3841 caaatgtcgt aaggtaaata aacttggcga agttgatctg aggcaatcgc cttacgatac
     3901 aactctgccc cggtattacg acctgttggg gtcatatggg tcaataggat tgtcttaccc
     3961 tcttttaata aagattcgat cagtacctga gtggcacggg tctcgccaac cgataccgca
     4021 tgaatccata cagcccgatg aaatgaagat ttgactccat aaaagccaaa gcgctcacca
     4081 atttttttaa ggtagtctgg attactgcga ctacgccaca acaaacgcac tagaacgaat
     4141 ggaatagcaa tgtgccaaag ggcctcatac agaaagtaga gaccaatagg taattttgtt
     4201 gcctgatgag atgaagcgcc caaaggttcc aattacttta aacgaagagc cagctctgcg
     4261 gccttaccaa cataagatcc tggggtcatc tttagtaaac gctttttctc gggctcaggt
     4321 atcggcaagg atttaataaa gcgatgcaag ccctctcggg taattccttt gccgcgagta
     4381 agctccttta attgctcata aggattttct atgccataac ggcgcataac agtttgtacc
     4441 ggttctgcca aaacctccca gcaatcatct aaatcagcag caatcgcagt ctcgtttact
     4501 tctaatttag cgagtccgcg taaagcacta tcgtaagcaa gtacgctgtg gccaaaggca
     4561 gggcctagat tacgcaatac agtggaatcc gtgaggtcac gctgccagcg cgagataggt
     4621 aatttttctg ctagatgacg taataaggca ttggcaatac caagattacc ctcagagttc
     4681 tcaaaatcaa ttgggtttac tttatggggc atggttgatg agccaatctc ccctgctttg
     4741 gtcttttgtt tgaaataccc caaggaaata taggcccaga aatcgcgatc catatccaag
     4801 agaatggtat tggcacgtgc tattgcatca aacaactcag ccattccgtc atggggctca
     4861 atctgaatgg tgtaggggtt aaattgcaaa cccaagcgtt gctccacaac ctgtttagaa
     4921 aacttctccc aatcgacatt gggataagca gctaaatgtg cgttgtaatt accgacagcg
     4981 ccattcatct ttcccaataa agcaatatct ccaatctttt ggattgctga ctctaagcgc
     5041 ttcgcaatgt tagcaatctc ttttcccaat gttgttggag atgcgggctg tccatgtgtt
     5101 cttgatagca tgggcagctt ggcatgttta acagctaggg tatttaatac cttaagaatg
     5161 ttctttagct gtggcaataa gacttgatcg cgtgcacctc gcaacattaa tccatgcgag
     5221 gtgttattga tatcctctga agtgcaagca aaatgaataa actcgctagc ctttaataat
     5281 ttagggcgct tcgcaacctt ctcttttaac cagtactcaa ctgccttgac gtcatggttg
     5341 gtaacggctt caatagattt aatgcgctct gcatctacct ccgagaaatc ttcagccagt
//
LOCUS       DDPQ01000138            4535 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_29261,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000138 DDPQ01000000
VERSION     DDPQ01000138.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_29261"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT         1330 a         1063 c         1071 g         1071 t
ORIGIN      
        1 ggccaaagga catacctcga ccttcaccaa gaacaggctt gaccttcctg tattacttgg
       61 tagaggtaaa gtcgtttggg tctctacgcg gtaatagaga tcatcgactg tattaggggt
      121 gcttgggttc ttgtatttag ggtgctggct taatttgggg ctgtttgtaa tggtccatac
      181 ataacgtcga aatgagccct gctctagatc gcccatcaca tgggctattt tggggctggc
      241 agccaccaac ttttgcccat cagcaacatg agcgtggatt tgtcctaagg gcatcccaat
      301 gcgttctgca ggcacccaac tgctcgggaa gcaaaaacag atagcgctca agaccccgtc
      361 ttgcatcaag gccacatctt cctcaagaca caaagccaga tcctcaattg actggatttg
      421 ctcaggctta ggcacgtgta gaacttgagc agcgtgctga agaagtggag tcagggtggc
      481 atgaggaatt tgtgcgcata gatcggcacc cagcgaggct agctctgttc tttttgcctc
      541 aagatacgaa gtccttgctg gtaaatgagt cagtgcgcct tgatgtcgca ccatgcgtgg
      601 tgccgtgctg tatggcacct caacaataaa gggtggtagc gagttcttat cctccatagg
      661 actctgactt aaatgcgttc tattttgatg cgattgatgg caaagaatga gccaataaat
      721 gcattaagga tagcgatgaa gatactagca aagaaggcgg tccaaaatcc ggccagcaca
      781 aaaccactca ccagttctgc tacaagcatt aagaccaatg cattaatcac caatagaaat
      841 aatcccatgc ttaatagcgt tagtggcaga gtaaacaaaa tgagtaaggg gcgcacaatc
      901 gcattcgcaa ggcccaatac caaagcagaa ataatcaagg cgcctgagtc attaaactgg
      961 agaccatcga agacataact tgcagcccat agcgatagcg aggtgatcgc ccaggtcaac
     1021 atgaataagg tcataacacc ccgcccttcc tgatttagag ttttagtatg agtaatatac
     1081 cgattattaa caaaatagag tctgatgcca aatcccatct ccaaaaaatt attgctctcg
     1141 aagtggactg cttgtaagcc caagaataaa gaaaagcact ttttagtgag caaaatccat
     1201 gatcccaatg tggaggagtc caatacgatc cccgcaggct ttgttgagct cgaagctgtt
     1261 atgagtaagc gttgctatct tctgccgatt gaggaactaa agaaccaaga tcaatggcaa
     1321 atcggctgga agcattaagt taatattgtc ttgttctgct ttattttgga gatatttatg
     1381 acccgtcgct catttattaa acaaaccggt ggtctcgcca ttgctggtgg cgccaccctc
     1441 ttatccagtt ccgctcttgc gcaatctgat accgttcgct ggcgctgtgc ctcgagcttt
     1501 ccaaagagtg ttgataccct atatggcact gcagaattaa ttgcccgtcg tgtgggtcaa
     1561 attacgggtg ggaagtttca gattagtgtg catgctgccg gtgagatcgt tcctgcatta
     1621 caggttcttg atgctgtaga gaaaggtacg attgagtgca atcacactgc cccctactat
     1681 tacattggca aagatcctgc ttgggcattt ggtacttgca ttccctttgg ccttaacagt
     1741 cgtcaataca acgcttggtg gatccatggt gatggagaaa agatctttaa tgagtttgcc
     1801 cgacagtctg gggtcattaa ttttctggca ggtaataccg gcacccaaat ggcaggctgg
     1861 tttaagaaag agatcaaaac ggtggatgat ctaaaggggt taaagttcag aaccggtggt
     1921 atgggtggtc aggttcttac taaactcggg gtcgttaccc aacaattacc tgccggagaa
     1981 atttactctg cacttgaaaa aggaactatt gatgcagcag agttcacggt tccctacgat
     2041 gatgagaaat taggtctcaa taagattgcc aagtactact actatccagg ttggaatgaa
     2101 ggcagtgcgg cattagcctt tcaggttaat gccaaagcgt atgacgcctt gccagaggct
     2161 tataagatgg caatccaagc tgctacttcg gaagccaata cctggatgca agccaagtac
     2221 gatgcattaa acccaaatgc attaaagcgt cttgtacagg gcggcgccat cttgcgtgct
     2281 tacccacaat cggtcatgga tgcttgcttt aaggccaatc aagagcttct tgaagagacc
     2341 agcgccaaga gtgcgacctt taaacggatt ctgacgcaaa tgcgtagctt ccagcgtgat
     2401 caacaagctt ggtttagggt tgctgagggc agttacgaca acttcatgtc tagacaaaaa
     2461 ctctgatcta cttgcgcttg aaataatcag caggatcttc tgcgggggca gcccgatttg
     2521 gttgcacccc actgtcactc tcgtcaagct tgatctcgat ctgctgtata tctacttcga
     2581 ggggcttatc caataggccc gtcacgatct gagggtaaaa gattaagatc aaaaccaaca
     2641 atagttgtaa gcctagccac ggaattgctc caagataaat atcactactc ttaatactgg
     2701 gtggtgcaat gccgcggaga taaaacagag caaagccaaa tggtggatgc atgaacgagg
     2761 tttgcatatt gacgcagatt aataccccaa accaaatgag atcaattcca agatccttag
     2821 caagcggtgc caatagcgga atcacaataa atgcgatctc aaagaagtca aggaagaatg
     2881 ccaagacaaa gatgatgatg ttcacaatga tcaggaaggt caccgcatca tcggccccca
     2941 tcgctgaaaa caaatgctca atccacttac ccccatccac tccctggaat accaaggtaa
     3001 acaccgtggc accaatcaga ataaagatca ccatcacggt aatgttcatg gtggattcca
     3061 taccttgcca taacatcttc cagtgaagtc ggcgatgcat cgctgctaag accatcgcac
     3121 caaccactcc catggcccct gcttctgtgg gcgttgcgag ccccatgaag atggtgccca
     3181 gtactataaa aataagtgcc atggaaggca gaatcgcaag tactacggtc ttgattaagt
     3241 cccaacccat tggtgttcta gcttctttag gtaggggtgg cattaattta ggattaatga
     3301 atgacaagat caagatgaat aacaagaaga ttaggatttg agcaatcgat ggtccgatgg
     3361 ctcctaaata catatcccct accgacttac caagttgatc tgccaagact attaagacca
     3421 atgagggagg gatcacctgc gtaatggttc ctgaggcagc tactacacca gtcgctaagc
     3481 gcggctgata gccatagcgc aacatcactg gcaaggcgat cactccgatc gcaatcactg
     3541 acgctgccac ggtaccggtg attgcgccta agatcgcccc caccacaatc acagcgtagg
     3601 ccagtccacc aggcaatcca ccaaagagtt ttgcaaagcc attaagcaaa tcttctgcaa
     3661 gaccactgcg ttccaggata gctcccatca gagtaaaaaa tgggatggac agtaaaagct
     3721 cgttcgataa gataccaaat acacgatacg gcagattagc cataaactgg gcaggaaaaa
     3781 taccaagctc aatcgcaatc agtccggaga tcaatccaag tgcagctaag gagaatgcaa
     3841 ccggtagccc aatgatcaaa aagaccacaa gggctacaaa cattgcaggg gccataaact
     3901 caagagctgg cattattggt ctgggcgttg atactgcata tccaatgtga tcttgccttg
     3961 taatgcagca agccgcttaa tgatctctga gattgcctgc aagatcagta agccaaagcc
     4021 aatgggcatt aagattttga caggccaccg tatgagcccg cctgcatttg ctgagcgctc
     4081 tccaatcgtc cacgcttcta ggtaccatgg ccaggcatag agaaccatca tgatgctaaa
     4141 aggaatgaca aaaaagagta ggccaaacag atcgagcatc atacgttggc gttgagcaag
     4201 gcgcagatag agtagatcaa ctcgaatatg accgttaata cagagtaaat gggcagcccc
     4261 caacataacg atgcccgcaa agagatacca ctgcatctct aaccaagcgt ttgagctcaa
     4321 atcgaatgca taccgtgata aggcgttccc caccgagata agtgcgcaga ccaagaccaa
     4381 tagcaccgca agctgggcgg cagtccgact gatacgatca atctgacggg atagcgcgag
     4441 taaatggttc atgaatgcat gggcaggaac aaaggtatga gtgtattgga tttatgccat
     4501 aagataccca agatgagctc cgagcgcctg attat
//
LOCUS       DDPQ01000139            4794 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_22529,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000139 DDPQ01000000
VERSION     DDPQ01000139.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_22529"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1797..1828
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1311 a         1118 c         1211 g         1122 t
ORIGIN      
        1 gttttcatat cggctgcacc gcgaccatac aaatggtcat cgcgctcact gggcataaag
       61 gggtcggtgg tccatttttc taggggtcca gttggcacca catcggtatg acctgcaaaa
      121 gcaagcacct tacctttggg gtcagcaccc cgtttgatag cccataaatt agtcacctga
      181 aaatcagccg gcccgctcac aatagtctcg gtttcaaaac caatggcggc cagacgcttt
      241 gcaatcagct cttggcaacc accatcggca ggtgttaccg aacggcaggc aattagttct
      301 tttgttagct caagggcgct ttgcatggct taatcgcggt agcttaatcg cgcaagagtt
      361 cattaatcgc agtcttagca cgtgtttgcg catcaacctt tttcacgatc acagcagcat
      421 acaaactgta cttgccgtta ctggcaggga ttgagccagg gaccaccacg gagcctgcgg
      481 gcactcggcc atagtgaacc tcaccggtct cgcggtcata gatcttggtg ctttgaccaa
      541 tatagacacc cattgagagc acgctgtttt cttcaatgac aacaccttcg accacttcgg
      601 agcgtgcgcc aataaagcag ttgtcttcaa taatcaccgg acctgcctga atgggttcta
      661 agaccccacc aatcccaact ccacccgaga ggtgaacgtt cttaccaatt tgagcacacg
      721 atcctacagt agcccaggta tcaaccatgc tgccttcatc gacataagcg ccaatgttga
      781 cgtaagaggg cattaggacc acattcttgg ccacataaga acctctgcgg gcaaatgctg
      841 gggggacgac acgaaagcca cctttggcaa agtcttccgg ggtgtagttc gcaaacttgc
      901 tgggcacttt gtcatagaac tgggtgaaac cacctgctga cattgtttgg ttatcttcaa
      961 gacgaaagga cagtaaaact gcttttttaa cccattgatt aacatcccac ttacccacac
     1021 ttcgccgctc ggcaacacgg atggtgccat tattgaggcc ttctaagaca gcattgaccg
     1081 cctgtctaat atcggcagga gcggagctag gagagagatt ggcgcggttt tcccaggcct
     1141 gatcgatgat ggtttgtagg gcttgactca tagggtgtgg ataactatag gattgataag
     1201 gatttttaga aaatacatgg tttaaatcat gagtctttca ttatattggg ggttgtacta
     1261 aagggttagt tgcccgcagg attgttatca tctcctctga gaacttttta atcctaaaac
     1321 ccctgatttt gtcccccaag aagaacgtcc gtgcaactta aatctattaa attatctggc
     1381 tttaaatcct ttgttgatcc tacccatttt gagttacctg gccagttaat tggggttgtg
     1441 ggcccaaatg gttgcggtaa gtccaacatt attgatgcgg ttcggtgggt gctgggtgag
     1501 tcgcgtgcca gcgagttacg cggtgagtcg atgcaagacg ttatttttaa tggctcgggt
     1561 ttacgtaaac cctctggccg cgccagtgtg gagctcattt ttgataatgc cgatggtcgt
     1621 gcgcatgggc aatggtcaag cttcacagag ttatctgtaa agcgtgtctt aacgcgtgat
     1681 ggcaactcca gctattacat caataatcag gtagtgcgtc gtaaggacgt gcaagatatt
     1741 ttcttgggta ctggtcttgg cccgcgtgct tatgccatta ttggacaggg aacgatnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa gtataaagag cgacgcaaag aaaccgcatc
     1861 tcgtctagaa gatactcaag aaaacttggt gcgtgttcaa gatatcttgc gagagctaga
     1921 gcaacaactt gctcgccttg aggcgcaggc cgaggttgct agccgtcaca atgagctctt
     1981 agcgcaaatg aagtcgcaac aacacctgct atggtttgta aaacaaaccg agtctggtaa
     2041 ggagcaagag aagcatgcaa atgcaatccg tgagacccaa gtcaagttag aagaagaaac
     2101 ctcgaaactg cgccatgcag aatctgaact agagaccatg cgcactcagc aatatgctct
     2161 gcaagacgtg gtctctaagg ctcaaggtga tctctatgag accaatgcag aagtgagtca
     2221 gatcgaatct gaaattaaat acgtgcatga gtcgcgccag cgtttacaag agcaggtcgc
     2281 tgatttgcaa gctcaactcg atcgttggac cactcaagag aaagatgcgg cacaagccca
     2341 gcgtcaggct gaacaagagt tccgtcaagc gagtgagaat gaggcaagct tgcaggcaga
     2401 gtttgtgcaa ttacagcaac gtttacccgt tcaagatcag gcctatcaat cggctagtaa
     2461 tgctctggac caagcacgct ctgtgctctc tggcatggag caacgtattg ttagcttgca
     2521 agagcgctat aaagcaggcg aatcacaaac tgaggaagtg agtggtcgtg caagtcgtct
     2581 gcgggatgag ttagcaggta tgcgtaagcc agatgatcag gcactccaaa tggctaccga
     2641 tcgccacatc atggcgcaac gtaaggcaga agacgcaaaa ttaaaggcca gtcagaccca
     2701 agagcatgtt cctgcgacgg atgctattcg tcaaaaagcc ttagagcaaa tccaaaaagc
     2761 caatcaagac gtacatcaaa gcgaggcaaa gttaactgcc ttgaccactt tgcaagccag
     2821 tgtgcaggcc caaggcaaga tcggtccttg gttggagagt aaaggcctga aagaaagtaa
     2881 acggctatgg caagagctca aagtagaaaa aggctgggag cccgcccttg agtctgtatt
     2941 gcgagagcgt ttagcggcat taactggtaa gagcattaat gagactctca gtcttgctaa
     3001 ggatgcacct cctagtcgct tagctatttt attaaccgat ggcacagacc aatcggactc
     3061 taaggtgccg gctggtttta ttactctttt atcccgcgtg caggcgagtg gaacaaccgc
     3121 aggcgttctt caagaatggc tcagcaatat ctatattgct gataatttag aggatgcact
     3181 tcgccgtcgt tcacaactaa ctaagggatc cgctctggtg acccaagagg gtcatatggt
     3241 gacccgagtg ggcgtgcagt tgtatgcacc agactctgag caagcaggaa tgttggcaag
     3301 ggctcaagag atggaaagtc ttgagaaaca actcaaggct caacagctaa tccaaagtga
     3361 ggttcaaagt gagctcgagc aggcgaacgc gaactatcaa gctgctcaac aagcggcgga
     3421 gcaggcacgg gttgcagccg atcagtcggt acaagaggcg cacagtttcg aagtagagaa
     3481 gatgcaatta ctgcaagcgc aagaacagta cagtacacgt gctgcacaga ttgatagcga
     3541 gcttcaagaa attgcgcaac agttaacgca ggttcaagat gccaatgcgc aaattaatac
     3601 acaactgacc aattccgagt cggagaagac cggtctccaa aatggcctta gttcggcgca
     3661 acaaaatctg gagcaagcag agtcagcccg tgagacttta cgcgagtcac tccgtcaagc
     3721 cgagatgacc gctcaagagg ctgcatttaa tacgcgctca ttacaacaaa agattaatga
     3781 tttacagcgt gatcaatcca ccgccagaac ccagatcatg gaactgcagg atcgctttga
     3841 tgcaagccaa gtagaactca aaggcttaag cgatgaggcg gcacaaacta aattacagtc
     3901 attactggtt actcgctccg agcgagaagc tgctcttgct aaagcacgga ccgagcaaga
     3961 agcaatgcag catcagttac gcgaggcaga tgaagcccgt ttatttttgg agcaaacctt
     4021 gcagcccatg cgcgatcgcg tagttgatct gcaattacgt gagcaagctg cacgcctaag
     4081 ctatgagcag ttcaccacct tattaaacga tgatgaggca gatctacagg ctttagcaga
     4141 gcgctatagc cccgatctac gagccagtag tctgcaggga gaggtaaccc gtctgaataa
     4201 cgagatccag tcactcggcc cagtcaatat ggcagcactc gatgagttat cgagtagtcg
     4261 tgagcgtaaa tctttcttgg atgcgcaatc tgctgacttg aatgaggcaa ttcaaacctt
     4321 aacggatgcg attgccaaaa ttgatgcaga gaccagagat cttctccaag gaacctttga
     4381 tcaagtgaac acccatttcg gacgactctt ccctgagcta tttggtggtg gtcacgccca
     4441 gttggtgatg accggcgaag agatcttgga tgcaggagtg caagtgatgg ctcagccacc
     4501 tggcaagaag aacagcacga ttcatttgct ctcaggtggc gagaaagcat taacagcgat
     4561 tgccctagtg ttttctctat tcttgttgaa tccagcccca ttttgcttgt tggatgaggt
     4621 ggatgccccg cttgatgatg caaatacgat gcgctattct gaaatggtgt ctagaatgtc
     4681 agataagacc cagtttgtat ttatctcgca taacaagatc accatggaaa ttgctagcca
     4741 attgattgga gtaacgatgc aggaacaggg cgtctcgcgg attgttgcgg ttga
//
LOCUS       DDPQ01000140           14099 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_3609,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000140 DDPQ01000000
VERSION     DDPQ01000140.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 14099)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14099)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14099
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_3609"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
BASE COUNT         3890 a         3313 c         3054 g         3842 t
ORIGIN      
        1 ctccgatctt gatgatattt atttttttgc tggccttggc gggcggcctt acctatatcg
       61 ccatccaaat acaagagctt gactcacgtg aaatgcgcac tagcgacttg gccaccgttc
      121 agaaacaatt ggggcaaatc caagatgagc tccatgagct tgatgagcga ctcgaagagc
      181 agtttgagtc tgtttttatt caacttgtcg agatgcaatc taaatatcaa agtttggcta
      241 gatcagcaca tatttctgct ccatcaaaac cagacccaac cgaagctgaa ttaaaaaagt
      301 ggcggcatct tggcgtagga caaaacaaaa acggggctta tgcattactt catgatgggc
      361 aacaagcact catgcttagt aaaaatcaaa tcgctaaggg atcatggcga ctcgctgaat
      421 ttaattcagc agaagcgatt ttagtcggcc ccaatgaaaa acgtattgtt ttaaggattc
      481 aatgagacgc tcctggctaa ctcattttct cggctgctta ttacttttaa tcagtgccgc
      541 ctgcttggct aacaatactc accggcaaac cgctgaaatc aaagagctac tccagacccc
      601 aattagcatt accgtcaatg aaatgctact ggttgattta ttacatatcc tggcacgtct
      661 tgggaacacc cattttgttt taagccctag tattcaaggc aatattagct tgcagctaga
      721 ggctgttcct tggcaaaccg ctctatctac agtcttagca agccgaggct tgcgctttgt
      781 tcgtaacgat ggcctatatt ggattgggag tcatagcgag attagtagtt ttcagaaaca
      841 acgtcgtgat gagtctgctt taccgtttgg gggagaacat cacagcacgc cgcgccaagt
      901 tttgattgag gcccgcattg ttgaggccga tcatcgcttt gccagaaacc taggtgtcaa
      961 attagggatg cgtccacgct cgtcttcttt agagcagacc ccatttcaga tgagcgtccc
     1021 actaaatgct ggtgggcttg gtggctatga gccagccagc gctgctgcca cccttttaag
     1081 taagaacgca agtcgtttgc tggatatcga actcactgcc ttagagagcg atggtcaagg
     1141 gaagattctg tcaaacccac gcattatgac cgcagatatg gtcaaagcaa ccattgagca
     1201 aggaactgag ctgccctacc aaacctcctc ctcaagtggt ggcaataaaa ttcagtttcg
     1261 caaagcaaat ttgcggctag aggttattcc aaagattcac catgatggca aagtttcttt
     1321 attggtcgcc attaacaaag atagtgtggg catgaagact gaacagggtt atgcaatcga
     1381 caccaaaaat ctgagttcag aagtaactgt tgaaaatggc ggcaccgtga ttcttggcgg
     1441 catctaccaa actactgaac gcgaagattt agtaaaagtt ccgcttttag gcgacattcc
     1501 cttaatcggt catctcttta aacaccaatc caagtttcgc gataaaacag agttattggt
     1561 ctttcttact cccaccattt tggacaaacc cgaatagaat tgaagggtga gtcctgaaaa
     1621 ttcaaatatc ttcctaatcg gcctaatggg tgccggcaaa tccactgtcg gtagggtgct
     1681 ggcacggaaa ctcaatcgtc gttttattga ttctgaccac gccctagagg aacgctgcgg
     1741 tgtgaagatt ccaactattt ttgagatgga aggcgaggcc ggttttcgta agcgtgaagg
     1801 gcaaattatt gaagagttga ctcaagaaaa aggtttaatc cttgctaccg gaggaggcag
     1861 tgttttgctc cctgaaaacc gtcgtgcctt gagcgaacgc gggacagtgg tctacttaca
     1921 tgccaaccct atcgaactct ggcatcgcac caaagggggg gaaggtcgcc ccttgttgca
     1981 aaatggagat ccaaaggcca tcttagaaaa tctgtatgct cttcgcgatc ccctctaccg
     2041 agaaattgct gatcatatta ttgaaacagg caaacccagc gttaaccaat tggtcaatac
     2101 tcttttaatg caacttgaac tcgcctcatg aaaacattac aagtctcctt gggagatcgt
     2161 agctacccta tttatattgg ctctggtttg cttggcaagc cagatctctt taagccccat
     2221 atagcaggtc agtccattta tgtcgtgagc aacaccaccg tggctcccct ctatagcaag
     2281 aaacttattc aaacaatttc aagagacgcc aagtctgttc atgaaattat cctgcctgat
     2341 ggtgagtcct ataaagactg ggctacctta cagaaaatct ttgatgtcat gctttctaag
     2401 ggggccgacc gaaagagcgt catcattgcc ttaggtggtg gtgtgattgg tgatatggct
     2461 ggctttgcag ctgctagttt tatgcgtggt atccgcttta ttcaggtacc taccaccctc
     2521 ttggcccaag tagattcctc ggtcggcggt aaaactggca tcaaccaccc cttgggaaaa
     2581 aatatgattg gcgcatttca tcaacccgtc gccgtcatag ctgatctcga taccctcaag
     2641 actctaccat ctagagagtt atcagccggc cttgctgagg ttgttaaaca cggtgcgatc
     2701 gccgatgcta ctttttttga ttggattgag tcgcatacaa aagagttgct ggggtgtgat
     2761 cccaatgcca tgagtcatgc tgtagaacgg tcgtgtgaaa ttaaatctgc tgttgtggca
     2821 gccgatgaaa aagaatctgg tatccgcgca atccttaact atggacacac atttggacat
     2881 gccattgaat ctggcttagg ttatggggag tggttacatg gtgaagcggt cggctgcggc
     2941 atggtgatgg cagcggatct atcagctcgc ctgggtcaca ttcctagcag tgatgtaacc
     3001 cgtattcagg aattagtgac tgcccttgga ttaccaaatt gcgcacccaa acttggtaat
     3061 cagcgcttct ttgagctaat gcaaattgat aaaaaatcag agggcggcga gattcggtat
     3121 atcactctgg aaaaaattgg gcgggccaaa attgagatgg ctgatcacgc aatcgttagc
     3181 caaactctta gcgcgtacca agcaagctga ccttaacgtc tttccccgcc tgagcgctaa
     3241 cgaaggatga taaatcagcg agtaactcat tgccggtttt agtatctaac caaagcggct
     3301 ccaatgccgt tccagcccaa cgataatgaa aaccgcctac cttggcggcg acccaaatct
     3361 catgaagagg gggctgggta ttaacgacga tgacgctacg atcatgaaag gcaatattga
     3421 taacattgcc accctgtcta gcgatatcta aatcaagatc caaagcctca tccgcatgct
     3481 cgagagagag ctcaattgag tgcaatagct gggctgcaat ctgatgaaac tgcttatcgt
     3541 caattttttc agcttggggt cgattggtgt tctgcatgct atattcaaat accttttgca
     3601 aagctaatga tgatatcgat tgtagtaaga attccgatac tggtgctttt tatttccctc
     3661 ggtgcttgtg gggtaagagg gcctttattt attcctaacc ctccccctat ccctccggcc
     3721 cctacccaag cagagcctaa aggaagccta tatccaccca aggatgtgcc agcaaaaaca
     3781 acgggaagcg gtaataaagt aaattaacaa tggtgaacgc aatcccctct cttgttggct
     3841 tctctgaaaa aaatgggcaa tggtttgctg agggaataag cctcagcaaa ctaacgcagg
     3901 attttggtac cccactatat gtttatagca aggaggctat ttgtagcgcc taccgcgcct
     3961 atgattctgc ctgcattagg cccgatggta cgcgacgagc tcgtattcat tacgccatga
     4021 aggcgaatag caatttagca gtcttagatg agcttaagca actgggcgcc ggctttgatc
     4081 tggtcagtgg tggagagttg gcgcgcgccc ttcatatcgg cgctgaccca aaaagtcttg
     4141 tttttgcagg tgttggtaaa tcatctgctg aaattgagaa cgcgcttcgt gtgggtgtca
     4201 aatgtattaa tgtggagtcc ataccggagt tacagcgtat taatgcaatt gctaaaacga
     4261 tgaatttacg cgccccaatt tcattgcgtg ttaatcctga tgtagatgcc aatacacatc
     4321 catacatctc tactggactt aaagataata agtttgggat tgcgtattac gaggttctca
     4381 aaacatatcg agaggctgcc cttttgcctc aaattgatgt gatcggcatt gactgccata
     4441 ttggttcgca aattacgagc acctcacctt atctcgatgc acttgataaa gttcttgatc
     4501 tcatcacaca acttcaacgc gaaggaatca agattcatca tctcgatttg ggtggcggat
     4561 tgggcattga ctatggcggt gagtcaccgc caaatattac tgaatttacc aatacacttc
     4621 ttaatcgggt ggaggagcgc ggatttggcc atctagaagt cttgctcgag cctgggcgtt
     4681 ctttagttgg taacgcaggt attttattga ctcaagtgga atacttaaaa acaggggtcg
     4741 ataaaaactt ttgtattgtc aatgccgcca tgaatgacct gatgcgacct gctttgtatg
     4801 aggcttatca tgcgattgtt ccgattcacg tcagccaagc taagccaaat tgctacgatg
     4861 ttgtaggccc cgtttgcgaa tctggcgatt ggctaggtaa ggatcgagta ctcgcggtca
     4921 acgaggggga tatccttgcg atcttgtcag ctggtgcgta tggctttgtg atggcctcta
     4981 actacaacac ccggggccga gctgccgaag taatggtaga cggcaatgag gcttatttag
     5041 tgaggcagcg cgaaacgcca gaaagcttgt atgaaaatga gcgcaccata aatcaagcga
     5101 gcgcgctcaa gtaagcagta aaagattaac gcagacctgc catatagtct gatactgcct
     5161 tcatttctgc gtccgatagc ttggttgcaa tcgtagtcat ctgagcacta tttttacgag
     5221 tgccctctct aaatgccact aactgcgaag tagagtaatc agcccactgc ccactcaatc
     5281 gtggatattg cgcaggaatg ccagcgccat tggggctatg acaaccagca catgcaggaa
     5341 cgcctttttc agcgatacca ccacgataga ttttttgacc aagtgcaatg ctatctttat
     5401 tttgagcaac ccctagggtg ggttgttgct tgtttaaata cgccgccaag ttcagcatat
     5461 cttgttgggt caaagttgcc accataccca tcatgacggg atttgcacga gagccgtcct
     5521 tgtagttttt aagctgtttc aaggtatacg ccgagtgctg cgctgccaat tttggccagc
     5581 ttgcaacccc actattacca ttggcgccat gacagccaac acaggcaacc accccacgct
     5641 tggggtcgcc attgttatag agtgcatcgc ctgccgccgt atctgccttt attgcagcag
     5701 gagcaggcgc agcagccatt gctggggtca ctggtttagc cggctctgtt gttgcattaa
     5761 caaggggtac aaatagcgtt gccaagacaa ctaaaaatga agggaagttt aatttggaga
     5821 ttttgcgact ttgacgcatt atggttacct tgcttacttt gatatgggta ctgaaatgat
     5881 cttaatttat tgagatttta caatagcttc catactttca tttatgtcta aacttcatca
     5941 aacccgcttc tttactaccg taaatgaact gcacaccctg ccccagggcg atattccgga
     6001 aatcgccttt gctggccgct caaatgccgg aaaatctagt gcaatcaatg tgttatgcaa
     6061 tcagaagcgg cttgcttttg ccagcaaaac tcccgggcgg acccaacata tcaactattt
     6121 tggggtttat gccaaagaaa cgctattggc ctatttggtg gacttgcctg gatatggcta
     6181 tgctgccgtc ggggaaagca cccgatccca ttggaacgat attctcagta actacctcca
     6241 gaaccgtaaa tcgcttgtag gcttagtttt gattgtggat gcgcgtcgcg gcctaactga
     6301 acttgatgag caaatgattc ggtggtttgg tgttacccaa aaaccgattc atatcctact
     6361 tagtaagtct gataaacaaa cctattccga aaaccgtgac ctcctgcata gtattaatga
     6421 acaggtaaag caatttgcgc cagcacaaat tagtgcccaa ctattttcta gtacaaaacg
     6481 ggttggcctt gaagaaagcg atacacttat tcaagaatgg ctttttaaag aaagctttct
     6541 tagtgaaaat actcctaact agtttgattc tttttaacaa atacaaccat gtctcaatac
     6601 cctgcctata gaccgcgtcg gatgcgtcgt gacgattggt cccgtcgctt aattcaagaa
     6661 aatcgtctca gtccagatga ccttatctat ccagtctttc tgctgcctgg taagaaccaa
     6721 acacagactg ttacctcaat gcctgggatt gaacgaatgt cactcgatcg tttattttca
     6781 gttgccgagg agtgtgtcgc tctaggtgtc ccggtcatgg ctttatttcc tgtgattgag
     6841 gcctcactca aaacgattga tggtgttgaa gcgtttaatc ccaatggctt agtgcccaat
     6901 gcagttactg aactaaaaaa acgttttccc aatcttggca tcatgaccga tgttgcctta
     6961 gacccctaca ctagccatgg gcaagatgga attttggatc aaaataaccg catactcaat
     7021 gatgaaactg ttgctgtttt agtaaaacag gctctatgtc atgctcacgc cggcgtcgat
     7081 attgttgcgc cctccgacat gatggacgga cgtattggac aaatccgagc cgctcttgaa
     7141 aataatcaat tcattcatac gcgcatcatg gcctactccg caaaatatgc gtcttctttc
     7201 tatggcccat ttcgggatgc tgtcggctct gccacaaatt taggtaaggc ggataaaaaa
     7261 aattatcaaa tggatccggc taatagcgat gaggctattc gtgaagtcgg ccttgatatt
     7321 agcgagggcg ctgacatggt catgattaaa cctggcatgc cttatttgga tatcgtccat
     7381 cgcattaaag atgcatttgg ctaccccacc tatgtgtacc aagtgagcgg tgaatatgca
     7441 atgctaaaag ctgctgccaa taatggttgg ttagatcatg atgcggtcat gatggaatct
     7501 ctgattgcat ttaagcgtgc tggtgcagat ggtatcctca gttattttgc tctcgaagca
     7561 agccgcctgc taaaaaaata atcgacagct cttcgaacta tttcgtattg agttcttgca
     7621 aacgttgact aaaacgtgtt ggcggcataa atccgacaat gcgcaatgct ttctgctctt
     7681 caccaaagct attaaacaat aagattgcgg gcggcccaaa aagaccatat tgctttaata
     7741 gggcctggct agatggatta ttcaccgtga catcagcttg aactaataca aaacgaccca
     7801 tctcttttgc aatctcaggg tttgcaaagg taatcgcttc catttcctta caactgatgc
     7861 accaatcggc atagaaatct aacaagactg gctttttatt ctcttgtgct ttttgtagga
     7921 tgatttgtag ttcttcgggg gaccgcacta cttggaagac aagatttgga gaaattcgtt
     7981 tttctgagcg attggtgttt tgcatgctac tttccccact gagcatagag aacgctggcc
     8041 atataatcca aattgccaag aacacaagca tcaccccaag tgcgcgctgc aaccaaatca
     8101 tccaagcacc ggcactcgga accacacttc ttgctcctaa ggcaatcagc aataagggta
     8161 agcccattcc cagcgccatc acaaataaca atgccccccc taacaccatg gaacccgttt
     8221 gtgccacaaa acccaaaacg cttgcgaggg gtgcggtgat acaagggctt gctaccaagg
     8281 ttgaaatcgc gccgagtaca aaagccccaa agaggcttcc acccttctgc ctgcctgcta
     8341 tttgatccac tttatcttgc caaacttgtg gcatttttag ttcgtagaac ccaaataaag
     8401 tccctgccaa aaatagcaaa agcccagaaa aaaggattaa agcaatcggg ctttgtagaa
     8461 cacgctgcac tccacttccc aaggctgcag tcaatatacc cgctaaggca tacagcaaag
     8521 ccatcccgag gatataagac aaggctaaat atgctgagcg aactttgctg attggcacat
     8581 cactttgttt acccaagaca atgctactta agataggtaa catgggtaat acacatggag
     8641 taaatgctaa tgctagtcct agaacaaaga agcctactaa caaatatcct aaattggcat
     8701 tttctagata tgacttaatt gaaccaacat catctcgtcc actccagagc tcaatcaaac
     8761 ttaatccctg aatattggca ccctctttgc tgggctttgc ctcgacctcc ggtattggcg
     8821 ccaccttcac tccaggggcg gcgataaaat attgataggt catcggtgga taacaaatac
     8881 cgccatcagc acagccttgt aaatctagtt ctaaacgaat gccctgttct gccttttgtg
     8941 taaaaggaat gatgacctga aagggctttg gataaatctc catcttttta ccaaaggtct
     9001 catcaaactt ttcaacaccg cgcggtagct gtatattttt tgtatttatt ttttgattgt
     9061 tgataaaaag tctgtattga agcgactctt gatagatgta atagcctttt gctggacggt
     9121 aatcaattaa caactcattg gcgtcggcta cccatgttgc ctctactaca aatgcttgtt
     9181 ccggcggcaa aaactcttta gctgaaagag tgccgatcaa aagcccaatg ccaatgatcg
     9241 gaatacaaat actcagaaaa attcttttga ttaggctcac ttaagctcct tagacaccca
     9301 agcaccatac gcttctgagt actggcttgg acttaaggca attatctctg gaagatcgta
     9361 tgggtggtgc tccacaatgt aactttctaa tgctggccaa cttgtctcgt ttgtcttggc
     9421 gcttaaaaga acttcagttt cttcacagat ttgatcgtcc cagcgataaa tcgaatgcac
     9481 gccctcctgg atttgtatgc aagccgctaa gccatgatca agcaagctac ggcctaatgt
     9541 cttggcctct tgaatatttg ggagggttgt gatcacgatc aaaatggaat tcatattgat
     9601 ttgattgctt gagctaattc ttagatccaa taaaaaagcc aggcaaaagc ctggctttac
     9661 tatacccgaa gcacgcttat gctgctgtct cttgggcagt ctcttcgact gctgggcgat
     9721 caactaattc aactaaagcc attggagcat tatcgccatg acggaatcca aacttcaaga
     9781 tccgcaaata accgcctggg cgagcagcgt accttgggcc taattctgaa aataatttag
     9841 ccaccatctc acgatcgcgc aaacgattaa atgcaaggcg gcgattagcc aaattatctt
     9901 tcttacccaa agtgatcaaa ggctcaacca ccatccgtag ctcttttgct tttggcaggg
     9961 ttgttttaat cacttcatgc tcgattaaag agtttgacat attgcgcaac atcgccaaac
    10021 gatgcgatga agtgcggttt aatttgcgta agccattacc atgacgcatg atgcttcctt
    10081 tctaattatt gcccaatcgt agctggaggc cagctatcga gtttcatacc aaggctcaag
    10141 ccacgggctg ctaatacatc tttaatctca ttaagagact tacgacctaa gttaggcgtc
    10201 ttcaatagct cattttcagt acgctggatt aaatcaccga tgtaataaat gttttctgct
    10261 tttaagcaat tggcagaacg aaccgttaac tcgagatcat caacgggtct catcaacaga
    10321 ggatcaacca tcgcggagcg gcttggcgct aagtcgccag caacttgact gctctccagg
    10381 gcggcaaaca caactaactg atctaccaga atactagctg cttgacgaat tgactcctct
    10441 ggagagataa ctccattggt ttcaatggtc atgaccaaac gatctaagtc agtacgttgc
    10501 tctacacgag cagattcaac agcatagcta acacgactga cagggctaaa ggatgcatcc
    10561 aacacaatgc ggccgattaa ctttgttgcc tcatcttgat attgacgcat attgccaggt
    10621 acgtaaccac ggcccttttc aaccttaatc tgcatatcca acttgccacc tgcagagagg
    10681 tgagcaatca catgctcagg attaatgatt tctacatcat ggggcaaatc aatatccttg
    10741 gcacaaacaa cgccagggcc ttctttacga agattgatcg ttacctcatc gcgtgattga
    10801 agcttaaata caacgccctt gagatttaat aaaagattaa caacatcttc ttgcacgcca
    10861 tccaaagtgg aatactcatg gacaacgcct ccgattgcca cttcggttgg tgcaaaacca
    10921 accattgacg acagcaatac gcggcgcaac gcattgccta gagtatggcc gtaaccgcgc
    10981 tcaaagggct ccataacaac cttagcctga ttagcagcaa gtgcttcaac agaaatgatt
    11041 tttggtttaa gcaaatttgt ttgcatagat atcttttcct taagaggtgc tgtattagcg
    11101 tgaatacaat tcgacgatca aactttcatt aatatcgccg ctaatttcat cgcgatctgg
    11161 aacttgttta aaagtacctt ctagttttgc agcatcaaca cttacccaat taatagcgcc
    11221 tacttgctgt gcaaggttta gagactcttg aatacgagtc tgttttttag ccttctcacg
    11281 aatagcaatc acatcacctg cacgcacttg catagagggg atattgacga cgctaccatt
    11341 cacagtaatt gctgcgtgcg aaacaagttg gcgcgcttcg gctcgcgtag agccaaaacc
    11401 catccgataa acgacgttat caagacgtga ttcgagcaat tgcaacaagg tttcacctgt
    11461 gtttccttta cgacgctctg cctctgcaaa atagcggcgg aactgacgct ctagaacacc
    11521 ataaatacgc tttacctttt gcttttcacg caattggtta ccaaagtcgg atgtacgtga
    11581 acctgaagtg cgtccatgtt gacctggttt gctatctaat ttgcacttat cagataaagc
    11641 acgacgtgcg ctctttaaaa ataagtcagt accttcccga cgggataatt tagccttagg
    11701 gcctaagtaa cgtgccacga ttccttcctt tctatgccgc agtttttcaa ctgtggtgag
    11761 ttcgcttcaa cttcaaacga acggtgggct tattaatcaa agactgaaaa tcagtcgctt
    11821 ctttacaact aaaaacgttt tatcaaatac gacggcgctt tggcgggcgg caaccattat
    11881 ggggaaccgg tgttacgtcc tggatctcca caattttgat acccaatgaa ttcaaggcgc
    11941 ggacagctga ttcacgtcca gggccagggc ctttaatttg tacttctaaa tttttaattc
    12001 cacattcaat cgcagcctta ccagcgactt cggcggcaac ctgagcggca aagggagtag
    12061 atttacgcga acctttaaac ccttgacccc ctgaagtcgc ccacgataga gcatttcctt
    12121 ggcggtcagt aatggtcacg atagtgttat tgaacgatgc atgaacgtga gcgataccat
    12181 cggccacatt ctttttaacc ttcttgcgtg cgcgttgtgc gctagcgtta gccgttgttt
    12241 gttgtgcttt tgccattttt tatcccttct tcaatgcaac gccggccttg cgtgggcctt
    12301 tacgagtacg cgcatttgtt ttagtacgtt gaccacgaac aggtaaaccc ttgcgatgac
    12361 gaataccgcg gtagcaggcc aaatccatca aacgcttgat gttcatggtc acttcgcgac
    12421 gtaaatcacc ctcagtggtg cgttttccaa cctcatcacg caatttctct aactctgcat
    12481 cggttagatc ttttactttt ttatcaatag caacacctgc gctatcgcag atcttcaatg
    12541 cttgcgtttt gccaatacca aaaatcgctg ttaagccgat tacagtatgt tgatgatttg
    12601 ggatgtttac cccagcgata cgtgccatat aaattcctct acttaaccga attaaccttg
    12661 acgctgctta tgacgtggat cagacgagca gatcacacgc actacgcgct tacgtttaat
    12721 aatcttgcaa tttctgcaaa ttgctttaac ggatgccaat actttcatga ttcacctctt
    12781 taataattac tttgcacgga agataattct tgcgcgcgtt aaatcgtatg gggtcatctc
    12841 cacggttacc ttatcgcctg gcaggatgcg tatgtaatgc atacgcatct tcccagaaat
    12901 atgacctaaa actacatgcc cattctctaa cttcacccta aacatggcat tgggtaagtt
    12961 ttcgacaatc tcacccgcca tctgaataac atcatcttta ggcatagtta aagtgtgggc
    13021 cccatcttaa agttagcttt cttcatcaga gagccgtact gttgttgcat cacaaacgat
    13081 tgcacttggg ccataaagtc cattgcaact accacaataa ttaataatga tgttccgcca
    13141 aaatagaaag gtacgttgta tttgagcact aagaactctg gcaataagca cactaaaacc
    13201 atgtaaatag cacctgctaa cgtgagacgt accaaaattt tgtcaatata tcgtgcggtt
    13261 tgatcaccgg gacgaatgcc tgggacaaaa gcgccactct ttttgagatt gtctgcagta
    13321 tcgcggctat taaaaaccaa agctgtataa aaaaagcaga agaaaataat tgccgctgca
    13381 tacaaaataa tatacacagg ctgtcctggt gccaaagtgg ctgctaagtc tttcacaact
    13441 cgactaacca tattggtagg ttcaccagca gtaaaccaac ctgctatggt ggctgggaac
    13501 aaaataattg aggatgcaaa aatggggggg ataacaccgg ccatatttaa cttcaatggt
    13561 agatgggaag actgtccacc gtaaatctta ttacctactt ggcgcttggc atagttgact
    13621 aaaatacggc gctgaccacg ctcaacaaaa actacaaagt aagttacagc aatcacaatt
    13681 aagacaatca aaattgccga taggatattc atggagccgg tgcgaaccag ttctaataaa
    13741 cttgccaatg cactgggtaa gccagaaaca attccaccaa aaataataat ggaaatgccg
    13801 ttacccaaac ctcgttctgt aatctgctca ccgagccaca ttaaaaacat ggtgccagtt
    13861 actagggtaa caacagtatt aaattggaac attgccccgg gatttacaac gagacctggc
    13921 tgtgactcaa gagctatgga aataccaatt gcctgaaagg ttgctaaaaa tacggttgca
    13981 tagcgcgtgt actgagtaat cttacgttgc cctgcttgcc cctctttttt cattgcctct
    14041 aacgatggaa gaacaatcgt catcaactgc ataatgatcg atgcagaaat ataaggcat
//
LOCUS       DDPQ01000141            4028 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_14638,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000141 DDPQ01000000
VERSION     DDPQ01000141.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_14638"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    2028..2102
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3696..3730
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1100 a          912 c          835 g         1069 t
ORIGIN      
        1 agggttttta ctagcctctt ctttattatt tcttttaacc ggtcatcatg ttgcagcgca
       61 atcacagagc caagcttcca ttcgactacc aacatcggtt gaccaagcaa tcaaacgaag
      121 tggcattagt aaggattcga tcagcatttc tgtgacggaa attctagtac ccactaatcc
      181 caaatcgcaa tctcgcacga ttctggcatg gcgtgatgaa gtaagcatga atcctgcatc
      241 aaccattaag ctgctaacga ctttggttgc attagatatt cttgggccta aatatcggtg
      301 gcgcacagat ctttttacag atggcgttat caagaatgga actctaaaag gtaatatata
      361 ttttttaggt catggcgacc ctaaattgat tccagaggaa ctcttaaagc taacaggtaa
      421 attacgagag cttggtatac agcatatcga tggtaatttg atttatgatc gcagtgctta
      481 tgccccccaa gtaatggagg atcagactat tgatggagag ttatttcggt cctacaacgt
      541 accaccggat ccacttttat attctttccg aaccctttct tttaagattg accccaataa
      601 aaatagtgat gctcttaata ttacttacac gcctagactt gcgcgcctta gtattcaaaa
      661 caatatttca gtcaactcaa atcaatgtga tgctggtaag cgtgatgtta atgttgagat
      721 aactcctgat ccaaatttag tttctggcaa tcaactcaag aaccaatctg caatacagtg
      781 ggtagccact tttagaggtg atctgtctaa aaattgtagg ggtatcggct acaacaccgt
      841 taaatttgac cctaatactt tttttactct tgggtttact gcagcctggg aagatgctgg
      901 tggaacttgg attaaatccc cacgcggttc ttctggaact gttcctgtgt atgctcgccc
      961 ccttctaagc tacgaaggac ttaatcttgc tgaggcttca caagatatta ataaattttc
     1021 taataatgtg atggcccgtc aagtgttttt aaccttggca cttgaaaaaa ttggtaagcc
     1081 cgctgatata cctggtggcg aaaaggtcgt taaagcttgg ctcatgcaac aaggcttatc
     1141 ttttcctgaa cttgttattg aaaatgggtc aggcctatct cgcaatgagg ctatctctgc
     1201 aaaaaatctg aatacactac taataactgc ccaaggcctt ccgatttctg aggtatttat
     1261 tgactcttta ccaatagcgg ggtctgaagg aacggtacgt catcgccttg taaaagaact
     1321 acgtaaattt ttacatctca agaaaaagcc cgaagttcga attaaaacgg gcgcacttaa
     1381 tcaagtaaga aatatttctg gctatgtctt tagcaagtcg ggtcgtattt atgcagtgac
     1441 atcatttatc aatgatccca aagctaatcg cggccaagaa atccatgatc aactactagg
     1501 gtggttaatg gaagacgggc cggatccaaa agaggcgcgc tgaagtcggt ctcgaattgc
     1561 atcccaaccc tcaccactcg gcgggcttgg tatgtagatt gtctgatact gggattgatc
     1621 tagaagacga agcaaacggt ataaaccgtg agcaaacgca atgggctctt ttggaacttg
     1681 gataagctta cttttcttgg ttagctcttt taattgagca agatcatgca agctctcatc
     1741 ttcccagaca actaaagcaa tactttgatc aagtattcnn aagtggcgta ttgggtgcat
     1801 agtgcgcctt caggctaccc gaaacgcgcg gtatcttctg atcctttata gggtcaatag
     1861 aaacattaat ccctgttgct tccttaattt gttttggact aatactgccc ggccgaagaa
     1921 gtcgtggctc cccatctcct gagacatcta agattgtcga ctcaatgccg aactgacaat
     1981 cacccccatc taagatcaat aaatcatctt gatctataaa ttctgagnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnccggttaa ggcatccaag accaatcggc ttttctgaaa aaccagggta agtgggccgg
     2161 gccaaaagtg ctcgataaga acaaaggctg attcgggaat atgccttgcc caaggactta
     2221 gaatggctaa ccaatcgtgg gctttatctt gtgaatgttc caatggcgct tggacatgaa
     2281 ctatgagtgg atggtttgct ggtcgaccct taagttgata aatcgcttgt atggccaagg
     2341 gattactagc atctgcaccc aaaccgtaaa cagtctctgt ggggatggcc acaagtttgc
     2401 cagcatgaag tgcgctcagg gctttggtaa cggactcgga atcagacaaa tgcttctcga
     2461 ataaatcgta agattatggt gcgataccta aatcatgggc caccgctgca caggcatctt
     2521 ttgcctcctg aataccctta cccagacaat taatatgtcc catcttgcgt cctaacttgg
     2581 ggctagcttt accgtacaaa tgtaatttag cactgggatg ggcaagtaca tgatgccagg
     2641 caggctctct cgcttgatct tgatcaaacc acacatcccc caaaatattc aacatggcga
     2701 ctggttccat aagggtgaca tcaccaagcg gcaagcgcgc catagccctc acctgttgct
     2761 caaattgact cgtaacagag gcatccatgg tgtagtgccc agaattatgg gggcgaggcg
     2821 ctatctcatt gacgagcact gagccatctt gcaagacaaa gtactcaaca cataaaacac
     2881 ccacatacgc catcttctcg gcgatttcaa agctcgctcc aataattttt tttgatacat
     2941 caggatgttt tacaagggat ggggatggca ctgtactggt atgcaaaata ccattacggt
     3001 gaatattttc agcaattgga taggccgtga cctgcccgtc atgagcacga acaattaatg
     3061 ccgagacctc aaatgcaaga ggcattcttt tttctaatac gcaagcattt ccaccaaggc
     3121 cctgccaagc aactgcaagc tcatccgcat ccctaacatt cacttgaccc ttaccgtcat
     3181 aacccattcg tgcagttttt aagattgctg gaaataattg ggtagggact gttgcaagat
     3241 cactttgcgt tttaatcaca tggtgtgggg ccgggccaat gcccgataaa acagagcact
     3301 gttggagaaa ttctttttca aggatccggt cttgggcaat cgaaacgcac ctgcccatgg
     3361 gagcaacaaa aatatcttta ttctgaaaat aatcaagggt cttggcaggc acattctcaa
     3421 actcggtact aactgctgca cacaagttag ccatttcatt taaggccttc tcatcaccat
     3481 aatctgcaca tatatgcttc tcagcaactg cacccccagg gcttagcgga tctggatcaa
     3541 gaacacatac cttatagcca agcgattgag cttcatgaat aaacatgcgg cccaactgtc
     3601 caccccctaa tattcctaac caagcaggcg ggagaatggg tgacagattc gttacagcag
     3661 aatttccaga cttcgtcatg atggaattaa gatgcnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn tcgtgcaatc gctttgctaa tgacgcatcc gagctggcca acattgccac
     3781 aatatgtaag gctgcatttg cagctccagc ctcaccaatt gcaaaactgg caaccggaat
     3841 tccttttggc atttgcacaa tcgagtagag agagtcttcg cctcgtaaat atttactagg
     3901 taccgggact ccccagatag gtacgatcgt ttttgctgca agcatgcccg gaaggtgggc
     3961 tgcgccacca gcgcctgcaa taatggctat taagccccgt gcttcagctt tctccgcata
     4021 ggcaaaca
//
LOCUS       DDPQ01000142            7564 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11877,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000142 DDPQ01000000
VERSION     DDPQ01000142.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11877"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    132..192
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5192..5214
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1953 a         1640 c         1911 g         1976 t
ORIGIN      
        1 gttgccatga ccacataggc ttcaccgatt gttctcccca gtgtcagggt gccatggttt
       61 cgtaagagca ttgcagggtg ctgacctaaa gactcggtta agcgttttcc ttctgtctca
      121 gtcagagcaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nngtgcatct ggtcttgcac gatgcactgc tccatgaatt gcaaagccgg
      241 tagggtttac tttgtataga gagtcggagt cgacaatctc accccgtgtg ttgaccttca
      301 ctaaattgga tgcagtaatt tcatcaaact ggtagccgaa tggattaatc aggtagtgat
      361 taggttcgcc aggaacgctg gctgagatgt gcgtataaat caaatcatcc cagccctcaa
      421 aggcgcaaag tcgatagcag gcagctaaat caatccgggt ttgccactct tctgggttca
      481 cgagttactc attatgggta tccgatttac tcggaaattt attcgaggca tagcggatca
      541 aggcattggc aagagctaat acaaaaatgg tgatacccat gagcagaatg cccatatctg
      601 cctcatgctt tgttccgact aaatcaataa tcaggcgagt aaccgctgtg atagcgatgt
      661 agatcaaaaa cctaaccggc atatggttgg tcttgaaata aatgcctacc atcgcaccaa
      721 tttctagata aataaagagt aggagtaggt cttgaacgga tgcataaggt ttttgcatca
      781 tttcagcaaa actcatgccg gcggcccaaa ctgtagctgc gccaatacca aaaagggcta
      841 tccggtggaa taaagaaaca aagagattac cggtagggat tagccaggat tcgatccggt
      901 gctcaatgtt tttaatgtct tttgggttac tttcactcat ggctttccct ttgttcggtt
      961 attttttagg ttcatatgcc ttatatttaa ccctaaatta acgacaagca gttaattgtt
     1021 actaagagga gcaaggtatg tctcgtaatt accccttttc agccattgtt ggtcaagagg
     1081 atatgaagtt agccattcta gttgccgccc tcgatccaag tgtcggtggt gtcttggtga
     1141 tgggggatcg tggcacgggt aagtcaactg cggttcgagg ccttgctgcc ttgttgccca
     1201 acatgactgt tgttcaaaac tgtccctatg gatgtgatcc aaaggctatg gcaggcgctt
     1261 gtgcaatttg cgatacgcag tccaattcca aaacaaaagt aaaaaccgaa gtacgccctg
     1321 tacccgtggt ggacttacct ttaggcgcaa ccgaagatcg cgtgcttggc gccttagata
     1381 ttgagaaagc cttgaccttg ggcgaaaaag cctttgagcc tggcctatta gcaagagcgc
     1441 atcgtggatt tttatacatc gatgaagtga acctactgga tgaccacttg gttgatcttt
     1501 tgattgatgt ggctgcttcc ggacagaatg tggttgagcg tgagggttta agtgtgcgcc
     1561 atccagcgcg atttgttctg gttggtagcg gtaatccgga agagggcgag ttacgtccac
     1621 aattacttga ccgctttggt ttggctgttg aggtaaagac acccggtgat atggcaggac
     1681 gtgtggagat cattaagcgg cgcgatcagt ttgaacgtaa tcctgaggcc ttcttagcag
     1741 tgtgggagaa agaagaagaa aagatccgcg gacaaatgac cgctgcccga aaaaaattac
     1801 ctactgtgaa agctgacgat aagttgctgg aagatgctac taaattgtgc gctcatcttg
     1861 gtaccgatgg cttacgtggc gagctcacct tattacgcgc ctcccgcgct gtagcagcat
     1921 tagaaggtaa aaataaagta agccgcgagc atctcaaacg aattgcagta cctgcattac
     1981 agcatcgctt acgccgaaat ccacttgatg agtctagttc agctacccgt gttcagcgcg
     2041 ctctcgatga attgtttgct taattaatga ctgaacactg gttggcttat ctgcagattg
     2101 cacaactgct tgtgctggcc ccgcatcact ttggtggggt gcttgtacgc tcacgttcgg
     2161 gcccggtgcg tgatcgttgg ttagtggccc ttgaacaaat ggctaaacag aatggcttat
     2221 cggtaccttt gcgcaagatt ccgaatgcaa ttacggatga gaacttactc ggtggcctcg
     2281 atattgaagc cacccttaaa caaggtaagc cggtcttgcg ggaaggagta ttggctaagt
     2341 gtgatcaagg tattgtggtt ttgccgatgg ccgagcgcct cgagataggc attgccgccc
     2401 ggatcgcaag tgcattagat catggggcaa tacagttaga gcgtgatggc cagagtaaaa
     2461 ccatggtgtg tcgtattggt gtgattgtgc tcgacgaaag tattgaagac gatgagcacg
     2521 tcaacccaaa attaatggag cgttgcgcct ttcacattga tctagatggg tttgcttggc
     2581 aatccctgcc ggatgaggag gtggataaag agtttagtga ttttgatcca agtcgagtgc
     2641 agactcgtct cgctgagatt gagctcgagg atgatcaaat tgatgcgctt gtggggatcg
     2701 caattcaatt aggaatcgca tccattcgtg cagtgcaatt tacagtgcgg accgcacgct
     2761 acttagctgc gctcgacccc ttgtgggaag gagtaatacc aagtagggac catatagagc
     2821 gcgcagtgag tatggttctt attcctcgcg caacgcaact gcccgagcag acccctgagg
     2881 agcaggcgaa tgaggagact ccgccacctg agcttgacga tactcgatct gaagaatcaa
     2941 atgcgccaca aacccccgat gaagaggtgc ttgaagatca aatattagac gctgcacgcg
     3001 ctagcattcc agctgacctt ttggcccgct tggcacaaat tgcgatggct agacagaaaa
     3061 atagttctgc aggtaaggca ggcgttagtc agatgagtag taaacgtggc cgcccaatgg
     3121 gttctatggc aggaatgcct agcgctagag gcacactaag tctgattgat actctgcgtg
     3181 ccgcagttcc atggcaagcc atgcggcgtc gcgagacggc gaacaaaaat cagattggcc
     3241 gcccatgtgt gctcattcaa aaagatgatt tccgtattaa acgctatcgt gagcgcacgc
     3301 aaaccttaac aacttttgta gtggatgcat caggctcatc ggcaatgaac cgtttagccg
     3361 aagccaaggg tgcagttgag cttcttttag cagagtgtta tgtgcggcgc gatcaagtag
     3421 cattgattgc gtttcgagga agtgaggctg agctgttact gagccctaca cgttcactgg
     3481 tcagagcaaa acgatctttg gcaagtttgc ctggaggagg aggcactcct ttagcgcgtg
     3541 caattgattc ggcatatcaa ctggctaaag cgtctcttaa aaaagggatg acaccaacgt
     3601 tggtgttctt aacggatggt cgtgccaata ttgcgcgtga tggcactcca ggtagggcac
     3661 aagcgcaggt cgatgcagag caatcggctc gtatggcagc gcaagtcaag gtacgtagct
     3721 tatggatcga tacttctccg caggcacgcg aggaaggtca acacattgct catttgattg
     3781 gatcctatta tttacctttg ccccatgcgg gtgcgcaaga actctcgcag gcagtaattc
     3841 aggtattaca tcagtaacga atcggttatt tttttagagt acgcagttca ctcaagatca
     3901 aatctgcact cttttcagtt tctacttcgt gcatgatgtg accaccctgc caatgaatca
     3961 cggttgattt tgcaaaatac tgttggatga ttttctgtaa acctatttca ggaacccatt
     4021 gatcactttc accaatgacc caaatgagtt tgctaggtag atttgagctg gcactaagca
     4081 cctgactgat attggctgaa gccataaagt tcattgcgcc acgtacgtga tcaggagagg
     4141 taaataaacg gcggtagtaa ttgcgattgg tctcgggcaa attggtattt gtagagtcca
     4201 gcaagcgatc ggtcatacca ctcatgggcg caatcttggc caaaatcgac gccagtgtgg
     4261 ctgattttgt aactgggcct aacatcggac caaaaaattg ggtgtagctg ggcggcggcg
     4321 ggatgagcga tggattaaaa ccaataatga tttgtggttt taccttggct tgaacggcaa
     4381 atgccagcgc gagcggcgca cctgcagaat ggccgaccac catggtcggc caaggaagtt
     4441 ttagctcttg aaccagacca atcaaactgc gggcaatttc ttcgagcttc aagctatcaa
     4501 taggagcccc taaagtaaag gcgtgccctg gtaaatcaag gttcaaaatc tgtgcctctt
     4561 tattgaggat gggcgttaac tctgcccacg aatgtgttga tgagccagtg ccatgcaata
     4621 acaaaatcag cggcccctta ccagagattt ggacatgcca agctagccca gccacctcga
     4681 ctgagcgact gatctgacga tttggccagt catagggaac gcgattcata gtgttcatta
     4741 agggttttcc cctagttaaa gtgcattgac ccaagtagat cgggtgtcta tattcgtaat
     4801 cataataagt gtaaaaaaat atttacaact attttaggtc ttaccagctc gtcaaccaca
     4861 ccagaaagta agtcatgagt ctaaaaagtg ctccaactca cgagatgcta aagcggattg
     4921 agaaaggctt gcatcaggct ttggcgatag ccgagtctaa gacagcccca caaaatttac
     4981 aaaaagcctt gcaccattct atttttccag gtggcgcacg catacgaccc caattatgtt
     5041 tggcagttgc cactgcatgc ggtgatgatg atcccgcatt atccactgcg gcagccagcg
     5101 ctatcgagct attgcattgc gcctcgttag tgcacgatga tttaccgtgt tttgataatg
     5161 cgctagtaag acgtgggcaa gcatcagttc annnnnnnnn nnnnnnnnnn nnnnttggca
     5221 ttccaatatg tagcgagttg tagcatgcgt tcgccattac gcttagctcc tgtgttgcgt
     5281 acgattgcaa catcggttgg cgcaccgcat ggaatcgtcg caggacaagc ttgggaatgt
     5341 gaatccaaag tgcaactcaa gcaatatcaa aaagagaaaa ctggatcttt atttgaagca
     5401 gcaactgcgg ctggagcaca ggcagcgggt gccgatgccg atacctggaa acccctaggt
     5461 gaatggttgg gcgaggccta tcaagttgct gacgatatcc gcgatgtatt agccgatcaa
     5521 gccttaatgg gtaaacccca aggacaagac attacgcacg acagaccaag ctctgcaagg
     5581 gatctcggcc tagtaggtgc tgtgcagcat tttgatgaat tggtatacaa agcaattaac
     5641 tcgatcccca gctgtaaggg cgagaagatg ttgcgtggct tagttgcaaa agaggctgag
     5701 cgattaatac ccgagtcatg gtttgcaaat acaaatcggc cattgcatca ccatgctacc
     5761 gtttgagcgt gtgtctggag acacgccccc ttcctctcat tcgctattcg atgcatttgt
     5821 agattggcga aattctttaa ttgcatcatc tcgctttcag agatgggctg ctcgttttcc
     5881 attcacccgg tggattgttc gtaagcaggc agcatccctt tttgatgtaa tggctgggtt
     5941 tgtatattca caaatattac ttgcgtgtgt gcgcgttaat gtatttgaac ttctagcaga
     6001 tggcgcatta tcaaaagcag atttacaaaa acagattgcg ctacctgagt ccggattaag
     6061 tcgtttacta gatgctgcgg tggcgattcg tttactcaca aagagaaaaa acggccacta
     6121 cgctttgggg atgttgggcg caccattggt tggtaatcgc gcattacttg acatgattat
     6181 tcatcatggc gatttctatc gcgatctaca agacccattg gcattattgc gcggtgatgt
     6241 ggctggtaaa gcagcgatgg cggagtactg gccctacatc aatggagaaa aggatccaag
     6301 tccagagtct ttatctgcgc agcgcgtagc ggactactca cgactgatgg cgcataccca
     6361 acctttggta acctccgagg taattgattc ctattcgttt gcaaaacaca aacatctctt
     6421 ggatattggt ggcgggcagg gcgcttttgt cagtcagtta gtagagctct atccccaact
     6481 tcaatgcact atttttgatc tcccgggtgt tgcagagtta gcaaatactc acattaataa
     6541 attaggttta tccaataaaa tcaatgcatt aggtggaaat tttttccagg gagagctacc
     6601 caaaggggct gatattgcca ctttaatccg ggttatcttt gatcatgacg acagtcgggt
     6661 ttcaatcctt ttgcgtaatg tattcaatgc cttagagcct ggcgctagcc tcattttggc
     6721 cgaacccatg gcgggcactc caggccaaga ggcaatggga gacgcctatt ttggcttcta
     6781 tttgcttgcg atgggtcgag gtcgaccaag aactcaggct gaaattagcg ccatgttgct
     6841 agccgctgga tttgagtcca tttctttatt gccatcagca atcccattaa atgcccagat
     6901 tttgcattgt aaaaaaccag tgttttcagt aagttagctt tttagagggg taaattccct
     6961 ataaagcctt gtaaatatta gggcgtatta gtagaaacac ctagatgaca ttgttgcaaa
     7021 agtggtgaag aatgcatata tgtaaataaa tatttacaca attagtatgt aaaaaagaaa
     7081 tgacactatg aacacaactt atcaagccaa agccgtcgtg ctcgagaagc ccgagagcat
     7141 tgtcttgagc gatctgcagt tatcccccgt aaccgaaacc gatgtcgtag tggatattga
     7201 gtggagtgga attagtacgg gcactgaaaa attattgtgg agcggacgta tgcctcactt
     7261 tcctggaatg ggatatccgc ttgttcctgg ttatgaatct gtcggaaaaa tttatcaagc
     7321 aggtatacgt tctggatata aaccagggca acgtgtattt gtgccaggtg cccgatgttt
     7381 tggtgatgta aaaggtttgt ttggtggtgc agcatcgcgt gtggtagtgc cggcgagtcg
     7441 agtcattgga gttgatagca aattaggccc agaggcggtc ctctttgcac ttgctgcaac
     7501 ggcccatcac gtgaccgcat cagaaggaag tcaacagcct gatctgatta ttggtcatgg
     7561 cgtg
//
LOCUS       DDPQ01000143           18439 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_5181,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000143 DDPQ01000000
VERSION     DDPQ01000143.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 18439)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18439)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18439
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_5181"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    223..253
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2122..2205
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2402..2549
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2708..2787
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3387..3466
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12106..12177
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17969..17991
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4379 a         4622 c         4179 g         4739 t
ORIGIN      
        1 atccgatgcc aacaccactg acttaaaccg tgtgccatca cgctcattcg ttccatttgc
       61 tttgaccaaa ccaaactcga tcttgcgcgc atctaaatcc acacgactta ccaatacatg
      121 caaacgatcc ccaatccgat accgaatacc ggtgcgctct ccgcggagtt cttggcgagc
      181 ctcatcgtat tgatagtaat ctccaccgag ctcggtcaca tgnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnccaaaat tagccacgcc tgtaatcgtt ccagaatatt cttggccgag
      301 atgatcgcgc atgtagtaac actttagcca tgcttccaca tcccgcgagg cttcatccgc
      361 gcggcgctca ttggcagagc aatgcacccc aaattgggcc cagaccgcaa gtgccgcacc
      421 gtctttggag acctgcttgg tcactgcttt cttacccttg gtcttggcat cctgttgcga
      481 ttgcttcaca gcggcagcat tggctctccc ctgacccttg cgtggcatgg tcaagttcat
      541 cggcacatcc tcgggcagat gcgggtgata actcttctta ttaagaatcg ctttaatgga
      601 gcgatgtact aataaatcag ggtagcgtcg tatcgggctt gtgaaatgcg catacgctgg
      661 gtacgagagt ccgaagtgac cttcgttctc aggctgatag atcgcctgct gcatcgagcg
      721 caagatcatc atctgtaaca tgccagcatc cggtcttgct ttaatctctt tcatgagctt
      781 ggcaaagtct ttgggatgag gcttatcggt acccgttaga tgcaagcctg tggttcgaag
      841 tacttgctta agggtttgca ccttctcgat cgatggctca ccatgcactc ggaacaaact
      901 aagatgctta tgcttttgta agaagttcgc tgcacaaaca ttagcggtta gcatgcattc
      961 ttcaatcaag cggtgtgcgt cattacgcag tcttggctcg atgcgcagta tcttacccag
     1021 ctcattacta atgatttggg tctcggtggt ctcaaagtca atggcgcctc gtttctcacg
     1081 ggcagcaaat aagattttat aaagatggta gagattattt aattgctcat taaactctgc
     1141 atagcgaact gcttcgggac cacgggtatt acttagaatc tcccagacgg tgtcgtaggt
     1201 aaagcgttgg gcagaatgca tcacaccttg atagaactga taggccagaa tctcaccacg
     1261 ctgatcaact acggcatcgc acaccataca caggcgatcg accccaggat taagagagca
     1321 caagccatta gagatcttct ctgggagcat gggtatgacc cttctgggga aatagaccga
     1381 tgttgctcgt agcaatgcct ctttatcaag tggatgatct ggtttgacgt aatgcgccac
     1441 gtcagcaatt gccacaatta agcgccatgc cttagcttgt ccatactgca ctggctcaca
     1501 atagaccgca tcatcaaaat ctcgtgcgtc agctccatcg atggtgacta agggaatatc
     1561 gcgcagatca acccgtccaa caagatcttc ttgggttact gtattgggta atgcctcaga
     1621 ctctttgaga actgccggcg agaacgtatg gggtactcca tatttacgca ccgcaatctc
     1681 aatctccata ccggggtcat cgatctcacc caagacctcg atcacacgac cgactgcttg
     1741 tcgatagcta tcggggtaat caataatctc aacgctcacc acttggccca gcttagccat
     1801 cccctgcccc tttggcggga tcagaatgtc atgaccaatg cgcttatctt cgggggccac
     1861 gatcagaacg ccgttctcgt tcagaagacg cccaatgacc actcgattgg cgtggatcaa
     1921 tacctccaca atttggccct cggggcgacc gcgtcgatcg gtccctaaaa cacggatgga
     1981 cactcggtcc ccatgcatga ccctagccat ctcacgctcc gctagaaaga tatcctcacc
     2041 gccgtcatcg ggcacgacaa agccaaaccc atcgcgatgg ccctgtacag tacctaggcg
     2101 gtcgccatcg cgtggtacag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnactgc ccagatggcg
     2221 aaattggtag acgcaccagc ttcaggtgct ggcgatcgaa aggttgtgga ggttcgagtc
     2281 ctcttctggg caccaaataa gaagttgttc aactttcaaa tggatgttgc aacaaaatgg
     2341 tctcatcgcg ctccggtccc gttgagacca tagcaatggg ttttccacag atctcttgta
     2401 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctctgggcaa aacatctaag gtcttaccgt
     2581 cgagcttata tcctacacac agacgaatgg ttttaatgcc atcaagtaca tctaatttgg
     2641 tgatgcacaa gcctgtcagc ccattaatct gaatagagcg tttgagtgcg gcggcatcca
     2701 accaaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnatt ccgacggggt cttgcttggc tgggttctca
     2821 tgatcgtaga gctcactagg gaatggtcca gcacccacgc gtgtgcaata tgctttggta
     2881 ataccgagga tgtagtgcaa ggatccggga ccaacacccg aaccggctgc agcattccct
     2941 gccacacaat tacttgaggt tacgaaggga taggtgccat gatcgatatc cagcaaggtg
     3001 ccctgcgcac cttcgaacaa taagttctgg ccagcctgct ctgctgcata aagagcgcta
     3061 gagacatcaa ccaccatgga tttaatacgc tctgcatatg acattgcctc ttcgagtact
     3121 ttcttgaagt ccaatgcctc aaccttgtaa tactgggtta gagcgaagtt gtgataatcc
     3181 aggttctctt tgagcttggc tgcaaattgg tctggataga agaagtcttg tacgcgcagt
     3241 gctcttctag ctactttgtc ttcgtatgct ggaccaatac cacgacccgt tgtaccaatc
     3301 ttatcgctac cgcgtttttt ctcacgagca tgatcaatgg caacgtgata cggcagtatt
     3361 aaggtagccg cttccgagat ctttaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccc caatgatgag
     3481 tgtgtgcccc gcattatgtc cgccttggaa acgcactaca gcttgcgcat gatcggttag
     3541 ccagtcaacg actttacctt tcccctcatc gccccattgg gtgccgataa ccacgacatt
     3601 acgtccatgc gaatgctctt gcgccatatt tattccactc tattgataga tgttttgttt
     3661 gatctgctaa gtgattgtat ttttattctt atttgcttgt tctatatttt tatttagtct
     3721 gcacttgcca gccggagccc tgcttcacta attcgcgatc gcatataaac tcctcagcac
     3781 ttggctgatc gccattgcgc aactgaatca ctacctctcc ctgactgcgt agatccgcaa
     3841 tcatggccaa tagtttttga tcatcagacc atggggcaat aatcgcactg cgcttgacat
     3901 tgaggtctga gcaatcggct aatgtataaa gatctaatga gaacccagtt gctgggcgtg
     3961 accgcccaaa tgcttgaccg acatgatcgt agcgaccgcc tcgtgcaatc ggctgtggca
     4021 aaccatccac ataggcagca aacatcacac cgctgtgata ttgatagccc cgtagatcag
     4081 ccagatcaat actaagctct agaccgttgg atgacagcgc ctctgttaat cgagcaagat
     4141 catttaaggc ttgctcaatg accgggttct taggcaatgc aaacttgcca gaacgcgcgt
     4201 tcgccagtac ctctgcgcat gagccattga gctcaatcaa ggcaagcaag ggcttgccaa
     4261 tcgaaggtgg catgttggta atgcactggc ttaagcctga ccgatctttt gtttgtaagc
     4321 aggtgtagag ggattcggta tcacttgcgt tgagcgcatg atctcccaag atgcctttta
     4381 agacgcccgc atgcgagaga tcgagataga cctttttaag gccagcctta ttgagggtct
     4441 gtaagagcag gctcagggcc tcaaggtcgg cctcccatcc tgcatgacca taaatctctg
     4501 ccccgatctg aagctcctca cgcgagctcg atccagctgg tgctcggaca tgggccactg
     4561 atccggcata gcaaagacgc gtaacgccct ctcgatttaa gaggtgtgca tcaatgcgtg
     4621 ctacctgggg ggtgatatca gctcgtaggc ccagggttct gccagatagc tgatcaacta
     4681 acttgaaggt ctgcagattg aggtcagacc ccgtccctgt taataatgaa tccaggaact
     4741 ctaataaagg ggggctaact aactcatagc cataagattg ataaagatcg agtgagcgac
     4801 gacgcaactc ttcaatcttg cgagcccggg atggcaaaac atccgcaata tcttcgggca
     4861 ataaccaacg gttcatgatg aaacttatct tctttgcatg aacttgaaga aatcattatt
     4921 gggctcaatc accataacgt ccttcttgtc cttaaaggaa ttgcgataag cctcaagact
     4981 gcggtagaac tgggcaaact gggggtctcg attaaaggct tctgcataga atgccgtagc
     5041 gcgtgcatcg ccaactcctt taatacgctg tgcatcgcgg tacgcttcgg ccaagattac
     5101 atcccgttgc cgttctgcat ttgcacggat cttatcggac tcagcagcac ccgtggaacg
     5161 gagttcgttt gccacccttt tgcgctctgc ttccatacga cggtataccg agtcactaat
     5221 ctctgctaag aggtcaacgc gcttgagacg cacatccaca atctcaatac caatatcttt
     5281 ggcatcttct tcgaccttct tacgaattcc ttgcatcacc tgatcgcgtt gctctgaaat
     5341 aatctcacgg atggtgcgct tcgtaaactc ttcattaagc gcagatcgca ctagttggtt
     5401 cattcgatcg gtcgctagac gctcatcccc tttaaagcta acgaagaact ttaaaggatc
     5461 gacaatccgc cacttcacat aagaatctac caacagattt ttcttctcag cggtaatgaa
     5521 gcgatcggca tctgggctat cgatggtcat gatgcgacga tcaaagaaaa tgacgttctg
     5581 aaatggagct ggtaacttaa attgcaagcc aggctcttga ataaccttca caatttgtcc
     5641 gaaagagaag atcactgcat atttacgttg atccacaata aagatgcaag aggtaattaa
     5701 gtagaacgct acgactaaac taacaatgag tccaaggaca cgatttggca tgatttattc
     5761 cctcgactct taacgatcac cgcgatcacg gctacgtagg ccatcgcgtt tttctggggt
     5821 acgatccgct ggtgctggat tagcactggg gcttggggcg gcaggcgctg ggatactcgg
     5881 caatccactt gtttgtgcct gcgcagcttg ctgagcttct gagctcacct gagaaataat
     5941 cttatcgaga gggagataca acaaactatt actcttggtg gtatcaacta atactttaga
     6001 gacattggta tagatctctt tcatggtatc gatatacatc cgatcacgtg ttacttgcgg
     6061 agcttttgca tactcggtat agatttgttt aaagcgcagc gcgtcacctt ctgccattgc
     6121 actcactcta gcgcgataac cctctgcttc ctgaatcaaa cgagctgcag tacctttagc
     6181 ccgtggcagg atgtcattcg cataggcctc accttcgctc tttaagcgct cttggtcttg
     6241 acccgccttc acggcatcat caaaggctgc ttgtacttgc tcagggggct gtacgttttg
     6301 gacggtaaca ctggtaataa agataccggc tttataacca tccaagatct tttggatcga
     6361 catggataaa tcgatcgcaa tcttctcacg accctcgtat aaaacggtat ccatcttgct
     6421 acgacccaca atctcacgca ctgcggtctc agctgcttgc acaaccgcca tatctggatc
     6481 gcgattatta aagagatact ccatcgggtc tttgagacga tattgcaccg caaagcgcac
     6541 atcgatgatg ttctcgtctt cggtcaacat cgaagaatct ttttgattcg ttcccttaat
     6601 cagaaccgga cgaccgatct ccacagagcg aactgcagac aaattaacaa tctcggtgga
     6661 ctgaatgggg aacggtaagc gccagttaat accaggacgg gcggtgtaat cgtatttacc
     6721 aaaggtcaat accacgccag cctgaccctc ttggatcatg aagaagccac taaagagcca
     6781 taccaagccc aaaataactg ctgcaccaat actggctact tttggattaa atccgtcggt
     6841 attaaattgt ggagttttgc gaccgccacc attgggtggg ggttgtccac ctccggatgg
     6901 gcgcttcttg ccaccaaaga gattattgag cttgtcatta aagtcacgcc acagatcatc
     6961 tagatcgggt ggtccactag tggggcgacg attatcttgc ggctgttgat ttggctgatt
     7021 gggggtagtg ttctcaccgc ccttggggtc tttatcccct ttgggtgcat tagattgagg
     7081 attattaccc cagcctggat cattgactga gtaaaaaccg gcaaagtaat cacgtacttt
     7141 acggagcgtt tggcgaatgg tttgggtttg gatggaaatc aaaatcggct gtttctggtc
     7201 tatcagttaa tggttcaagc aggtcgccac tggcccttaa tttttggact aaagcgtgct
     7261 caacccttag tttatcagtc atctgcgcaa actcggtaag gcattgtcgg agtaggtcta
     7321 aacccattcc ctcttgggca gaaataaaga ccttatatgg cagcccatta atgtcgcgct
     7381 cgatctgcgg gcccttagta aaggtctggg gcatctggtc gattttgttc atgacctcaa
     7441 tgcggggaat ggtatcggcc ccgatttctt tgaggacagc ctctacttct gcctgttgct
     7501 gaagagccac agggctacag gcatcgacca catgcaagat caaatctgca tgaacggtct
     7561 cgtccaaggt tgcccgaaag gcttccacta actggtgggg aagctcccga ataaatccca
     7621 cagtatcgga gaggacaatt tgcatgccct cgcccaaatg gattcggcgg gaggtcgtat
     7681 caagggttgc aaatagctga tcagcggcat agctaccagc cttggttaga gcattaaaca
     7741 gtgtagattt acccgcattg gtgtaaccca ccaaggagac cgaaaatact tgattgcgcg
     7801 cacgggctct gcgttgcgtg cgctgttgac gttgnnaact tctctaattc tgcctcgagt
     7861 cgctttgctt tcgtggctag catgcggcga tctagctcca tctgggtctc accaggcccg
     7921 cctcgaagac caataccgcc cttttgacgc tctaagtgac tccaagctct tactagtcga
     7981 gacatgcgat agcgcacatt cgctagctca acctgtgttt tacctaaatg acttttggct
     8041 cgttgggcaa agatatccag tatgaggccg gtacgatcaa tgacatgccg gcctagatga
     8101 cgctctagat tacgttgctg cgtaggcgaa atggcatggt taacgatggc cagttcggca
     8161 tcgtgttctt tcatggtacg taggagttcg ttggctttac cagaacgaat aaagagagct
     8221 gggtctacgc taccacgtcg tcccaccact gaagcttttg gtatggatcc agcgctttcg
     8281 gccaagagcg ctagctctgc catgctctcc ggaaaatcct ctttaccggt gtcaatgccg
     8341 actaaaacag cacggacctc ttctacaccc gtattatgca gatggagctt cctcttcgtc
     8401 tgcctttaag ctcactgctc gagctggcac aacggtagag atggcgtgtt tgtagaccat
     8461 ttgggtgacg gtgttacgca ataagatcac gtattgatca aaagactcga cattgccctg
     8521 caatttgatg ccattgacca aatagattga tacaggaatg tgctctttgc gcaaggcatt
     8581 taagaatggg tcttgcagga gttgggcttg tttgttgttc atactgctcc tttttctact
     8641 tattttgctt agtttgctgt gggagtcttg cttttatagt tgcaatagta ccgtttacct
     8701 taaagatatt accctcatta aataaattcc accacgaaga aaacataacc tacttacgac
     8761 gtggtttttc ttctttcaca taagggtttg cggcagttcg gaactcaatg cgcaagggag
     8821 tgccgcgcag atcaaaaatc tcccgaaaac gcccttctaa atagcgctta tagctatcgg
     8881 taatgccatt taaggcgctc ccatgaataa tcacaatcgg tgggttcatg cccccttgat
     8941 gggcatagcg cagttttggt cgtccacgac caacgcgttt aggttgctga tgcgctaccg
     9001 catcgatcat cgcccgagta agcttcggcg tagagagttt ggccatgcct gccaagtaag
     9061 ccttgtccac gtccttaaag agatctttaa ttccaaagcc ttttttggca gaaataggat
     9121 gaacgttagc aaagtccaga aatcggagtt tttgggcgat ctcaacacgc gcccgttctt
     9181 tgacataagt atcaatgcca tcccacttat taacggccac caccatcgcg cgccccgcct
     9241 ccacaatata tcctgcaata tgtgcatctt gctccgagat atcttgctga gcatcgagca
     9301 tcaagatcac cacattggca tcattgatcg cctgcaatgt tttaaccacc gagaacttct
     9361 cgatcgcctc aaatactttg ccacggcgac gcagaccagc cgtatcaatc aatatgtagg
     9421 gcttgccatt gcgttcaaag ggcacttcaa ttgcatcgcg ggtcgttccg ggtagatcaa
     9481 acgcaataac ccgctcttct ccaattaagg tgttaatgaa ggttgattta cccacattgg
     9541 gtcgaccaac caccgcaatc ttcattggtc ggccgctatc cacaacagac tcttcatctg
     9601 gcactggata tgggatctgg agttgatcca acgcttcctc tagtaaggag cgcacaccat
     9661 ctccatgggc tgacgagatt gcctcgggct cacccaaagc tagctcatgg aaatcagcgg
     9721 tgaccacgct gtgtggcata ccctctgttt tgttgaccgc cagaatgacc gggcgaccgg
     9781 tcttgcgcaa gaactctgcg atcactcggt cttgtggtgc tagacctaag cgaccatcga
     9841 ccaagaagat gatgagatcg gactctgcaa cggcttgttt ggtttgctta gccatctccg
     9901 ccaagatgcc ggtcttggca acaggctcaa agccgccggt atcaatgcaa atgaactcac
     9961 gctcgcccac tcggccgtta ccataatggc gatcgcgggt cagtcccgga aaatcagcta
    10021 ccagggcatc gcgagattta gtaaggcgat tgaataaagt ggatttaccg acattgggtc
    10081 ggcccacaat tgcgataacg ggtttcatga atgtgttctt agcgcggacg gtaagcagca
    10141 atcttgccgc cctgcgaggc gatgatgatt acgccaccag cagcaatcgg cgcggcactc
    10201 accggggagc tgtcatgccg aatgcgcgag accatctcac cactattttg attaatgagg
    10261 tgcacataac cttgcttatc acccatcagc actaccttac caatcgccag tggttccccc
    10321 acatcgcgcc aggtcagttg cgtgttttgc cagacctgta caccatcgct tgcacgatag
    10381 gcagtgacgt gtgacttctc atcggcagca aagacgaact caatactttg cgctgttccg
    10441 gtgtagctcg agaaatcttt tgaccaaact aagttaccag tgcgtagctc agcacagcca
    10501 atctttcctt ggtaagagac gatgcataaa cgaccttcct ctatgctagg tcgagtggtg
    10561 acatcattaa gacgctcaat ctccgagaag cccttaggaa aagaaaccgc ggactcccaa
    10621 atgagaccgc cattgttcag ggccatcgcc ccaaagcgac cacctgcaaa acctgtaatg
    10681 accgcatcat tacctaccaa gagcatgccg tagccgaccc ttagggacag cggcgattgc
    10741 tgacgttgat aaatccaacg acgcttaccg ctagcggcat ccaagccaat aaaacggtta
    10801 tctagggtcc gaatcaccac aatgccgcca gcgacgatgg ggtcggaaag tacctcgcta
    10861 ccgatggatg atttccaaat atttttgcca gcctcatcgt acgcaaagac attaccttcg
    10921 ctactgatag caaccgtggt tttaccatcg gagccaggcc caatcgagag cttttctgga
    10981 acagaaactt cccacactac tctgccggtc tggatattta atttactgag ggtgccacgc
    11041 atggaggcag caaagacatg atcatcggcc accacgggac gaaagtttgc aggcacagag
    11101 ccacctacac tggcagacca tacttctacc aaatctacct gattggtgag gttcactaaa
    11161 tcagcaggct tgcgaacctt tgatgagccg gagcaggcca agagggttag cgaaaccaat
    11221 aatacggagg cggtgcgcac aaattgcact tacatacccc ctactgcatc gatcttgacc
    11281 ttcaagagac gcttggagtc ttcaggaaag gatttatctt tctcgagtaa tttccaggca
    11341 tctaaatatt gggttcgtgc ttcagtggtc ttcttttggg ccaagaacca atcaccgcgc
    11401 cgctctatcc acaagggctc aaatcctttg accgccttcc cttttagaat tcgatcggca
    11461 tcactcatag ccgcatcact accctgatcg atcaatagac ttactaatcg catttgcgct
    11521 aatgcccgat aggcatcatc gcttgcttta tctgcagtcc actttaaata cgcttgtgct
    11581 ttggcaggat cattggagtc cgaggcaatc ttggcagcca gcaaactacc catcgctgca
    11641 taggtggtgc caccgtattt tttttgaagg tcatcagcag caagaaatgt tttatctcgc
    11701 tcgtttttac tgagggtgcc caataaggtc tcatagagtt ttgcggcttc agccgattgg
    11761 ctgcgctgat accagctcca gccactcatg gcagcatatg taaagagggc taccgttgcg
    11821 acgccaataa tgtatttacc gaagcgattc caaaaggact tcagatttgc aatttgttct
    11881 tgttcttcga gatcgagtgc catcgtgctg tgctgcctgt atgtataagg atttgaataa
    11941 acggctattt tatgcccatg ggcttagtga tgcccatgcc cagcattatc aacgatcaac
    12001 caaggcatca atcacggcac tcaaaagccc tgccagggga atggcgcgtt gctcaccggt
    12061 accccgtaga tctttgagtt gcccttcctg acgggccaac tcatcnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg
    12181 ccatcgggtg ggcaatgcaa aatcacatcg acccctgcac tgcgcaagga ttcagcagca
    12241 atcaaggctt gcgttaaggt ctcgccaccc tgatgcaaga cataaacatc acaaacgggt
    12301 tcggtctctg gcaagctctc tgagataccc attaactcaa tcacgcgctc catgcccata
    12361 gcccatccac aggcaggagc aggtttacca cccattcgct caatcagggg atcatagcga
    12421 ccaccgcccg caatcgtgcc ttgggctcct agggcatcgg tgatccactc aaagacacta
    12481 aggttgtaat agtcaagacc acgtaccaaa cggggattga tcttgaaagg aatgttatta
    12541 gctttcagta gactctgtaa ggtctcaaag tgttggagcg actctttacc caagaactct
    12601 agtaattttg gtgcgccctc aatcagagtt tgcatggcag ggttcttcga atccaagatg
    12661 cgcaagggat tgctttgcaa tcgtcgatgt gaatcttcat cgagttgaga ctgatattgg
    12721 gtgaagtaac tcaccaaagc gctacgatgc gcagctcgtt catcggcttg acctaaagag
    12781 ttaatctcaa ggcgcacgcc ttttaagccg agctcatccc agaggcgctg ccccatcaga
    12841 ataatctcag catcgatatc gggtcctgca aatccgagcg cctcgatgcc aaattgatga
    12901 aactggcgat agcgcccacg ttgtggacgc tcatgccgga acatcggacc ggtgtaccaa
    12961 agacgtttgg gtccctcata aatgaggttg tgctcaatca cggcacggac gatcgctgca
    13021 gttccctcgg gacgcagagt cagttgctca ccgttgagtc gatcctcgaa ggagtacatc
    13081 tccttctcca caatatcggt gacctcacca atcccgcgtt ggaataaagc tgttgattcc
    13141 acaatcggcg tccgaatcgc ttcgtaccca tacgcgcggg tgagatcaga aagagccctc
    13201 tctaagtgca accatgctgc cgactgactt ggcagcatat cgttcattcc tcggatggca
    13261 ctgatctttt gcatgagatt gatgattgaa taaaactgat tagcgatagt gcgtgttgac
    13321 gtagtcatcc actatcagct ggaactcttc tgcaataccc tcgcctcgca aggtttttac
    13381 tttcacgcca tctacaaaca ccggcgcagc gggggtctca cccgttccgg gcaaagagat
    13441 gccaatgttg gcatgcttgc tctcgccagg tccgttcaca atgcaaccca tcaccgcgac
    13501 gttcatgttt tctacgcccg gatgtgtttt tttccagact ggcatttgct gacgcaaata
    13561 ggactgaatc ttggcagcca gctcttggaa ggtggtgctg gtcgtgcgac cgcaaccagg
    13621 acaagcaatc accatcggcg tgaaattacg caagcccatg gtttgcaaaa tctcttgccc
    13681 cacaatcact tcattctcac ggggtgcacc cggatcagga gtcagtgaga tacgaatagt
    13741 atctccaatc ccttcttgca ataagaccga cagtgctgcg gtcgatgcca caatcccttt
    13801 gctacccata ccggcttctg tgagacctaa gtgcaaggag taatcgcaac gacgtgccag
    13861 atcacgatag accgcaatca gatcttgcac tccactgacc ttgcacgaga gcgtaatttg
    13921 gtcggggttc atgcctaact caacggcctt actagcagat tgcaaagcag actgtattaa
    13981 tgcctcaatc atgacctcct gtgaggtctt agggcttgct agtttggcgt tgtcgtccat
    14041 gatctgggcc aatagatctt ggtctaggct gccccagttc accccaatcc gaatcggctt
    14101 gtcgtaatgg catgcagcct caatcatctg tgcaaactga gggtctcgtt tagcgccctt
    14161 gcctacatta ccggggttaa tgcggtactt cgataaagat ttagcacact ccggatagtc
    14221 ttttaataga gtatgtccgt tgtaatggaa gtccccaatt aagggcacca tgacatccat
    14281 cttatcgagc tgctctcgga tgtaaggaac ttgagctgcc gcctcaggtg tattgacagt
    14341 aattctgact agctcagagc cggcgcgagc cagttctttc acctgaattg ctgtagcaat
    14401 ggcatcttcg gtatccgtat tggtcattga ctggacacga accggtgcat cgccaccgac
    14461 actaaccttg tgatcgcccc aagctacaac ggattggcgt gagatcctgc gagccaatgg
    14521 cccaaggggt aaaggagcta aacaatgatt tggcgtactc ataatctctc tattatcgtt
    14581 cctgccattg caccacatgt tcgctgcgca ttgcccgttc ttggcgttga ttgcgattgg
    14641 tgcgatcgat cacatcaccc gccagctgac cgcaggcagc ggcaatatca tccccacggg
    14701 ttttacgaac cgtggttact atctgtgcat cttgcaaaat ctgggcaaat tctttaactc
    14761 gcgctggagg tgagcgcttt aggcctgact ctgggaacgg attaaatgga atgaggttga
    14821 gcttgcaatg caagcccttt aataaacgaa taagttcttt tgcatgctga tcctgatcgt
    14881 tcacgccatc gagcatgcag tactcaaagg taatgaagtc tctgggtgca aatggtaggt
    14941 agcgatcgca ggcggcaatg agctctttga gtggatactt ttgattaagc ggcaccagtt
    15001 cattgcgcag ggtatcgttt ggagcgtgca aggagaccgc taatgcgacg gggcaatcct
    15061 gtgccaagcg atccatcatc ggcaccaccc ctgaagtaga tactgtgacg cggcggcgcg
    15121 ataagccata agcatgatca tcgagcatca gacgcaaggc gattaataca gaatcgtagt
    15181 tgagtaatgg ctcgcccatt cccatcatca ccacattgga gatcacgcgg cccgattgtt
    15241 gagtcgcaca atcttcatct agacggcgga tggcattggg atcttgacgc aaatgatgct
    15301 ctgcaaacca gagttggcca ataatctcag ccggtgttaa gtttctggca aagccttggt
    15361 gcccggttga gcaaaaccgg cagttgactg cgcaacctgc ttgggatgag atacagagtg
    15421 tgccgcgatc atcttctggg atgtacacca tctctaccgc attaccggct ccgacatcca
    15481 gcaaccattt gcgggtccca tcgtctgccc agtgatcttg gatcaccggc aagctcacta
    15541 cctcagcatc ttctgccaag gtggtccgaa atgatttagc aagatcggtc atcaatgcga
    15601 catcgctgat gccgcgctga tgaacccact gcatcagttg ttttgcccga aagggctttt
    15661 cgtttagtcc cgcaacgtac tctgccatct tggcagcatc aaagttgagt aagttcgtgc
    15721 gggctgtgtt caagacaatc gattaacgcg aataaatggt catcccgggg aagaaaaatg
    15781 cgacttccac ggcggcggtc tcaggggcat cagagccatg cactgcgttt gcatcaatgc
    15841 tgtcagcaaa atcagcacga atggtgcctt tgtctgcctt tttgggatcg gttgcgccca
    15901 tgagctcacg gttcttcaga atcgcgttct ctccctcaag cacctgaatc atcacagggc
    15961 cagagatcat gaagctgacc agatctttaa agaaggggcg atctttatgg acagcgtaga
    16021 actgctctgc ctcattctta gaaagatggg ccattttgga tgcgatgatc ttcaaaccgg
    16081 cttgctcgaa gcgagcatag atttggccaa ttacattctt ggcgactgca tcgggtttga
    16141 tgattgagag ggtacgctga attgccatgc aaaaactcca gtaataagga tttaataaaa
    16201 ggggcaactt gctaactgtt gcgatgcgaa attatacgtt gtttttaggg ttttagcccc
    16261 atcaaccccc tctaagccat tgaatttctt acgtataagc cataatttcc gtaatggtat
    16321 ttaggcggtc ttgattttta cccattttgt ctatacttag tccctataaa gcccgtgttg
    16381 ggccttttat aaggagctac tcaatgagtg atctgaactc gtatggtttt ggtaatggcg
    16441 caacagttgc gaccgcccag acccgtaacc gtgtcctaag aaacacctat gccctcttgg
    16501 cattgtccat ggtgcctacc gtcattggcg catggttagg tgttgcgatg ggctttacct
    16561 tctttgaagg cagtccattt atgggagcca tcattttctt agcagttgcc ttcgggttct
    16621 tctgggcaat tgaaaagaat aaagatagcg gacttggtgt tgtcctgttg cttggattca
    16681 cattcttcat gggtgtgatg ctttcccgct tgatcggtat gacactaggc agctacagca
    16741 atggcgctac cttgatcatg ggtgcattcg gtggcacagc ggctatcttc acggtcatgg
    16801 cttccattgc aaccgtctcc aagaaagact tctctggcat gagcaagttt ttgttcattg
    16861 gcgtgatctt gatgatcttg gcttccttgg caaacatttg gttacaaatg cctgctttga
    16921 tgttgacctt gatggtcgtg gccattgcga tcttctctgc cttcattctg gttgatgtac
    16981 aacgtgttgt taacggtggc gagaccaatt atgtgattgc tacccttggc atttacttaa
    17041 gcgtctacaa catcttctcc aacctcttag ccctcttggg tatctttggt ggtgatcgcg
    17101 actaatagca attagttcag ctgttgttct tagggcgcct cggcgccctt tttattggtt
    17161 cgctagcact ttgcaagcat taaatttcct ttattaatct ttttttcaat agttgcaata
    17221 gatttagact cacgcttaat ctcaccaagc actaaattaa taaaggtttg ccagccctgt
    17281 cccttggatt tataaaactt caatgtatct ttatcaattc gtagggtaat ttgctcctta
    17341 ctgccactgc cgacaggcct accgcgcccg cgaacgatat ttggcttaat acgtttccac
    17401 tccttttcag taagtggacg actgtctggg tcagacctgg cagccttagt aattaagata
    17461 tcttccttgg cgcttggttt aatcaatttc ttcttcataa taagtaagct cccgcctatt
    17521 tgctttacgt aaactaatga ttctccttgt tgattctaag atcacataca caacataatg
    17581 cagccttctt ttcaatggta ctaaacctac ccatctctgt tctcggtaat tttttctgtc
    17641 atcaacccaa aaaattcctt cttcccagtt aagattttcg gcttcttgta atgcgactcc
    17701 atgcttttca atattttttc tatttttttc taggtcaaat ataaatttca ttatatttat
    17761 tgtagctaca ataaatatat tatgcaatta ttataactac aaaaataaag ggtatttatt
    17821 tagtctcata gtcaaacacc gcaatcgatt caatatgcga tgtgtggggg aacatattga
    17881 tgatccccgc cttactcagt cggtaattca gctcatggca gagaatggcg gcatccctag
    17941 cgagggtttt gggattgcac gatacatann nnnnnnnnnn nnnnnnnnnn ngtctttggg
    18001 aagatcatgc atggctttgc agatctccat cgctccttca cgaggggggt caattaacca
    18061 cttatcggcc ttgccccagc tggcgatatc tgttgccgtg gtctcaaata gattactttc
    18121 ttgaaagcgt gtgttctgct ctagacgatt atgcgtggca ttttgcattg ctcgcttgct
    18181 taggattgca ctgccctcaa tccccaagac ctccttggct tgagtcgcga ttggtaaggt
    18241 gaaattacca atgccacaga acaaatcaat aatgcgttca ttgggtttaa tctctaatag
    18301 tgatagcgcc ttgctaacca agacttggtt taccgcatgg tttacctgcg tgaagtccgt
    18361 cggcaagaat ggcatctcga tcccaaactc gggtaagcga tagagcattt gctgctcgct
    18421 cggataaaac ggtttagcg
//
LOCUS       DDPQ01000144           20871 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_13144,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000144 DDPQ01000000
VERSION     DDPQ01000144.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 20871)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20871)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20871
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_13144"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    213..237
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    717..775
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    979..1049
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3875..3909
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4431..4495
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9567..9604
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11337..11419
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12641..12677
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16761..16840
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17865..17893
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18533..18603
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19725..19837
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5286 a         4501 c         5092 g         5284 t
ORIGIN      
        1 gccggcaaga ttgaatcaat ccccgctgta gttgcctgct ttgagtttga gtacatgggt
       61 ggctcgatgg gctctgtagt gggtgagcgc tttgtgcggg gtgtgcaaga agcgattaat
      121 aaaaaatgcg cgttcatttg cattacagca acaggtggcg cccgtatgca agagagtcta
      181 ctttcactct tgcagatggc caagaccaat tcnnnnnnnn nnnnnnnnnn nnnnnnnatg
      241 ggtgatgtag tgatggccga gcccaaggct ttaattggtt ttgcagggcc gcgcgtgatc
      301 gagcaaacgg tgcgcgagaa attacccgaa ggctttcagc gctcggagtt cttaatgcaa
      361 aagggtggta tcgacatgat tgtggatcgt cgagagatgc gtaaagaaat tgcgcaccta
      421 ttggctctat tacaaagtat cccagccgat aaactcccca agcaattact caactccagc
      481 ggttcgagct aagtcgtgcc ggagaacagc cagaacaaag ccattctctt taccaatcta
      541 gatgactggt taacgtattt agagaccgcc caccctgtcg ggattgatat gggactcacc
      601 cgtattaatc aggtgaaaga gtccttaggc ttgcgtttct cctgccccgt tattaccgtg
      661 ggcggtacca acggtaaagg ttccacctgt gcatttctag agagtgttct cttagcnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtgc
      781 tcggattcat ggtcgtgaag caacggatgc tgagctcttg caatccttca atcgggtaga
      841 gcaagcacgg tgtcggattg agaatgctcc gaccttaacg tattttgagt tcaccacctt
      901 agccatcatg gatctctttg ccaatgctgg gattgatgtg gcgattttgg aagtagggat
      961 gggtggtcgc ttggatgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggggaacacc cgcgaggcca tcgcttttga
     1081 gaaagctggc atcttccgca agaacgcaag tgccatttgt ggtgatccag taccgcccca
     1141 aagtttgatt gatcacgcta aggctttggg taccgatcta tggctgatcg gcaaggattt
     1201 tggttttgag ggcgatcaac agcaatgggg atggtccgga cgaggaaagc gctttagtgg
     1261 tttggcttat ccagcattac gaggtgccaa tcaattactc aatgcctctg ccgtaattgc
     1321 tgccttaatg gccttgcacg atcgtttacc agtgagtgcg caagatatac gcaatggttt
     1381 tgccttagta gagttaccag gacggtttca ggtcttaccc ggtaagccaa gcatcgtctt
     1441 agatgtggca cacaatccgc atgcggctgc taccctcgca caaagtttgg ataagatggg
     1501 ttatcacccc tatacctatg cagtatttgg tgccatggct gataaagata ttgagggggt
     1561 gctcaagccg atgatggatt tggtggatta ttggtattgc accgatttgc caacagcgcg
     1621 cgcagcgaaa gggatagatc tcgccaaaca gattcaggga ctgggtaaag aggcagaggt
     1681 attttctggg gcgggtagcg cttatcaaat ggctttggat aaagcgggtg agggtgatag
     1741 aattgtggtc ttcggatcct tttatacagt tgcgggcgta atggcttatc gaaataacca
     1801 ggcacattga tccatgacct tcatatcgaa tctatttcga cgtaaatccg accctcaaaa
     1861 aattgagccc ctcgctaggc gcccaattac taaacgcacc agagccaatg aagatccggc
     1921 tgaagatcca gccttaaccg aagatcccga gaaacaacgt gcgcgccatc gcttggttgg
     1981 tgcagcgact ttggtgctga ttgcagtcat tggtttacct cagatcttgg ataaaaaacc
     2041 taagcccgtt gttaatgaca ttacggtgca gattgtgagt agcatcccga atcctgccgc
     2101 cggtatcttg aacccagagc cagacaagaa agcaaacccc gagccaaaac tagagacaaa
     2161 ggtcgagacc aaggctgaac caaaacctga cctcaaaccc gagaccaagc ccgtaacacc
     2221 acccccaggg actaatccag cgaataaggg ttttgggctt gcccctggtg aagagttggt
     2281 tgtgattagt aaggatgcag ctgctagcgg tgagatgaag gccgcagcca atggtaagtt
     2341 tgtgattcag attggggcgt ttgcatcgga agagcgtgcc aatggttggg ttgcaaaact
     2401 caaagataac aagattccgt attacgtact cactcgcaat aatgctgagg gaaccaaatt
     2461 gtttgtcttg cgtgctggcc catttgtaga gcgcgaagca gcagaagcag ctgagaaacg
     2521 tatcaaggca ttggggctca gtcctcgaat cgtagaactc aataagaatt aatgatggaa
     2581 cagctcagcg ccattcacct gaccaccctc gattactttg cgttcggtat tttggtgatc
     2641 tcggcagcgg tgggcgtatg gcgaggttta tttagagagg tgattgcatt agcctcttgg
     2701 ttcattgctg catggctttc ttatcattac tgtgattatt tggcgagtga gtggctgtct
     2761 gccttcatac ccaatgcaac cgcccgctta gcctcagcgt tcttgttaat ctttgtgatt
     2821 gttctcatca gtcttggttt attggctaga gctctgcaaa aagtattatc aagcgccggc
     2881 ttaaccttag ttgatcgttt ctttggcctc gttttcggct ttgcacgggg catagtggtt
     2941 ctggtagttc tagcaacatt aggcgcatta acgggtgcac aacaaaccca agcatggcaa
     3001 gcagccgcga ttcggccaac ccttgaaaaa ggaactggct taatacgctc atggttgcca
     3061 gagagctggg ctaagcagtt aaatgcaata tcaatgaccg attcaactcg gtcgccgggg
     3121 agctaaacat gtgcggagtg gttggaacaa tctcgcatca accagtaaat caacttttgt
     3181 atgatgcact tttgctcttg cagcatcgtg gtcaggatgc agcaggtatt gcaaccatgc
     3241 acggtaactc ctttgcgatg cataaagcca atggtctagt acgcgatgtc tttagaaccc
     3301 gtaatatgcg cagtcttgtt ggaaacgctg gtattgggca ggtacgttat cccacggccg
     3361 gttcagcgag cagtgaagaa gaggcgcaac ctttttatgt cagtgctcca tttggaatca
     3421 tcctcgctca caacggcaat ttaactaacg caccgatatt acgcagcgag atggcctacc
     3481 gcgatcgtcg ccatatcaac accaactcag atactgaagt gctactgaat gtcttggccg
     3541 atgagcttca gaaagaaacc aatagtgctg cgctagacga cgactcgatc tttaaggccg
     3601 tagtaggact taataaacgg gtgaagggct cttatgcagt tgtggcctta attgcgggct
     3661 ttgggctttt agcattcaga gacccatttg gtatccgccc actatgcatt ggtcggatcg
     3721 atacaccgca aggacccgag tggatgctgg cctctgaatc tgtggcatta gaaggcctag
     3781 ggttcaccgt cgtgcgcgat gtgcaaccgg gtgaggcgat ctatattgat atggatggta
     3841 atttccattc tagacagtgt gcaagtaatg catcnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnc gtatgcgcat gggtgattac ttggcagaga aaatccgagc agaggccgac
     3961 gttagcaaaa tcgatgtggt gatgccgatc ccggattcga gtcgtcctgc agcaatgcag
     4021 gttgccaaac gtttagggat ttcgtatcgc gagggcttct tcaagaaccg ttacattggt
     4081 cggacattta tcatgcccgg acaagcagtg cgcaagaagt cggttcgtca aaaactgaat
     4141 gcaatgcgca tcgagttcaa agataaaacg gtattaattg ttgatgactc gattgtgcgt
     4201 ggcacgacct cgtatgagat tgtgcaaatg gctagagagt cgggtgccaa gaaagtaatc
     4261 tttgcttcgg ctgcgccccc agtacgattc cccaatgtgt atggcattga tatgccaact
     4321 cgtagtgagt tagtagctta tggtcgcact cacgatgaga tcaatcaatt aattggggct
     4381 gatcagctga tctatcaaag tgtcgaggat atgaaaaaag ctgtacaaga nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncattt
     4501 gcaattagca aacggagact aatcctcctc attgtggttt gtgtggcacg ctgaggtgac
     4561 agttcggtaa ttccctgtca aactagagtt cttatgaaaa aaaccatccg cgccaaaccc
     4621 aatatgaaca ccctggcgcc tgagaccatt gcggttcggg ccggtacgcg tcgcagttcg
     4681 gattatcaag agcattctga agctatgttc ttaacctcga gtttctgctt taactccgcc
     4741 gaagaagccg agcatggttt tgcccacgcg gatcagggct ttatctattc acgtttcaca
     4801 aacccaaccg tgagtatgtt tcaggatcga ttggccgcac tcgagggtgg tgaggcgtgt
     4861 attgcaacct catcgggtat gtctgcaatc ttaacaactg caatgtccca ccttcaggcg
     4921 ggcgatcatg tcatttgctc caagtctgtc tttggggcaa ctacgcaact cttctcatcg
     4981 atcttgggtc gctttgggat tactacgacc tacgtggcga ttcatgatgt caaggcgtgg
     5041 caagcagcgg tccaaaaaaa tactcgcttg ttctacttag agaccccatc taatccctta
     5101 actgagattg ctgatattgc agcaattgct aaagtggcca agaaagcaaa gtccttattc
     5161 gtggtggata actgtttctg cacgcctgca ttgcaaaagc cattagagtt gggcgccgat
     5221 gtcgtaattc attcggcaac caagtatctt gatggacaag gtcgtgtcgt gggcggtgcg
     5281 attgtgggca gtacagattt tgtgatgggt aaagtatttc cctacgtgcg tactgcgggc
     5341 ccaacacttt cagcctttaa tgcttgggta ttcctaaaag gtttggagac cctcgatttg
     5401 cggatgaagg cgcaaagtag taatgcactc gcacttgcac agtggttaga aaaacaagct
     5461 ggagtagaga aggtatttca tccgggatta aagtcgcacc cgcaacacgc attagcaaag
     5521 cgtcagcaaa aagccggtgg tccgattgtg tcttttgtct taaagggtgg caagaaggcg
     5581 gcctatcaac tcattaataa aacacgcctg tgttcgatca cagctaattt gggcgacact
     5641 cgcacaacca ttacccatcc cgcaacgaca acgcactgtc gtattacacc gcaagccaga
     5701 aaagaggcgg gtattgttga tggcttagta cggattgcgg tagggcttga gaacatcgaa
     5761 gatctcaaga acgatttaca agccggcctc aaaaaataat gagctgctag ggcgtcgcat
     5821 tcgctgcgct cataaagcga cgtggatcat taatggggcg cagaggcatg atgtggatta
     5881 aatctcgatt gaactctaaa tagaccaaag catcttgctc taaaccaagg cgtctaactt
     5941 cagcagcaga tgcttcccag gtaagtgttt ctgtaatggc ttcaatcaca aattcgatta
     6001 ctgcaatttg tccgagagta cttatttgct ttactcgaca gggaatgcgt ccatcgagtc
     6061 gattggaatg aacacttaag cccgcttgcg gaatgaccca agctacttgc gattgcggcg
     6121 ggatcttgcc cttgtcggtg atctggaaag cccaggggct cgagccccac tgtaatcgcc
     6181 ccgcattaaa taccccctga aacagattgg ggatacctac caattgagca actctcggat
     6241 tacggggctt cgcaaataaa tcattggcgt tggctgtttg caaacccact ccttgatcga
     6301 tcacggtaat gcgatcagcg agcaaatgcg cctcacgcag atcatgggtc accaacacaa
     6361 tcggaatatg caattgcttg cgtaactcag caagcgtctg gtagagagtt tgtcgggttg
     6421 gtatatcaat cgcagagaag ggctcatcga gcaataagat tttgggagag cgcgctaatg
     6481 cgcgggcaag ggcaacacgc tgttgctgtc ctccggaaat ttgcctaggc aggcggtcag
     6541 caagctcagc aatattaagt tgcgccagcc aatcgtgagc gcaccgtatt tgctcagcac
     6601 gactttcacc attgttatgc agagcaatac gaacgttctc taaagcactt aggtgcggaa
     6661 acaaagcata ctgctgaaat aaaaatccac atgagcgtac cgaggcgtct tggactatgc
     6721 gagggccgcg ggcaggatcc cgatttagcc acactgtatt agcgcattcg atgctgccca
     6781 tctcggggtg atgcaatcct gcaatcgagc gcagaacact tgttttgcca ctccccgaag
     6841 gtcctaccaa tgcatggatc tcgccatagt ccacctctaa atgaatagag agtggattgg
     6901 gtacagtctg ctgaatatgt aacttaagca cgatgagatc ctgctttggg ttttccgaga
     6961 acaccatacg agatcgcaat cgcaatgaga gagatcagca acaagatcaa agacaaggat
     7021 cctgcacctg ccgtatcaaa ggtctggacc ttgtcataaa ttgcgatgga ggctgtttta
     7081 gtctccccag ggatagcacc acccaccatc agcaccacac caaactcacc caaggtgtga
     7141 gcaaaggtca tggcagcacc actgagaata ccgcgccaag ccagtggtaa ttcaattaaa
     7201 cgaaagcgtt gccaggtatt gagaccgcta accttagcgg cctccttgat ttctgggggg
     7261 attgcctcaa aggcacgctg aattggctga atcgcaaacg gtagattgat gatcagagag
     7321 gctaccaata agcccgtgaa cgaaaacacc aagggctcgc caaataaact tttaccagca
     7381 aaagccacaa taaagtaata gcccaagacc gtaggaggta agactagggg cagggcaagc
     7441 gcagcctcag cccaagattt ccagggaccc gcatcggcaa gacgatgcgc tacccagatc
     7501 cctaatggaa tcagaaggac taaggtccaa aaggccaatg ttagtgagag ggcgatcgct
     7561 tgggtgtcta gcaatttact ttatttaatt taattaaatc agttacttaa gaaatatgtt
     7621 atttaggata atgggctatt ttaggctcta ggcgatgctt gtgggttgat ctttcgggat
     7681 gctaattagg gtatctctgt atatgcataa agacaaatat ttcgtgtaat atacgcaaat
     7741 acgcatattt atggctagcc ttccaaagac ctcaaaaaag actccatcct tggagatcag
     7801 tgaggcgttt gtaaaaaaac aattgagcaa gcagcaaatg gaatctctat ttgtgggcgt
     7861 atcgcaattt ttctcaacct tatcagaacc atctcgcctg cgtatcttat acgcgctatg
     7921 tgaaggcgaa aaatctgtga ctgaagtgat tgaggcgtgc ggctccagtc aggccaatgt
     7981 atcgcgccat ttgtctgcat tacatcaagc caaaatatta aaccgccgta aggttggcac
     8041 tacagtcttt tatgccattg aagaccaagc gaccttagag atctgccaaa acatctgtgc
     8101 gcgcattgcc gaagaacttt tttctgaaaa gagttaatcc ccatttaatt tctatccccc
     8161 atactgcaat gaacaaaact tcccccaaca cccaggccct tgagtccttt agtcgtattc
     8221 agaaggcccc cgagaacttc ctctcgcaag acgccattgc agacattact cagcatggtg
     8281 ctaacgagga acgtcgtgga ttcttgcgca aaagttttgt tgcagccctt ggtggagcca
     8341 gtgccatggg tggtggtctg gccatggcga accccaaagg cgatcccgct attttggaga
     8401 aacaagaatg gcaaaccacg cttggtaaaa acgtagcaac agatccttat ggaatgccat
     8461 cggtctatga gaagaatcta gttcgtcgag agtcgcctgg tctgacacgc gtatcggcag
     8521 catcggttgc tttcacacct ttgcaaggac tctttggcat catcaccccc aatggtttgc
     8581 attttgagcg tcaccatcag ggttggtaca accttgatcc aaataaacac cgtttgatga
     8641 ttaatggttt ggtgaagaac gaaaaggtat tcaccatgaa tgacctgatg cgcctaccgt
     8701 cggtctcgcg tattcacttc attgagtgtg gagccaatac cggtttggaa tggggcaatg
     8761 ttgccgtacc gactgtgcag tacacccacg gtatgttgtc gtgctgtgag ttcacgggcg
     8821 taccactctc tacactcctc gatatctgcg gcgcagatct taaaaaaggg aagtatgttt
     8881 tggcagaggg cggcgatggc tctggcatga cccgaacgat gtcgatggaa atggccttgg
     8941 acgatgtgat tgttgcgtgg ggtatgaacg gtgagatgct gcgccctgag aatggattcc
     9001 cattgcgctt agtggtgcct ggtgtgcaag gcgtgagttg ggtgaagtgg ttgcgccgta
     9061 ttgaagtcgg tgatatgccc tatgctgcga aggacgaggc gattcattac atcgatctca
     9121 tgcccgatgg cctgcatcgt caatacacct cgattcaaga atgtaaatcg gttatcacca
     9181 caccatcggg tggccaacaa ctcctggaca aagggttcta taacattagt ggtctggcgt
     9241 ggtcaggacg tagcaagatt aagagggtgg atgtttctgt tgacggtggc aataactggc
     9301 gtactgctcg tttagagacc cctgtgctta ccaaagcgct tactcgtttt aatatcgatt
     9361 ggacctggga tggttcaccg accatcttgc aatcccgcgc aattgacgag accggttatg
     9421 tacaaccgac catacgacag ttaagggcag tacgcggtac gcgctcgatc tatcacaaca
     9481 atgcaattca atcgtggaag gttgatacca atggcgaggt cagcaatgtt caagtcggct
     9541 aatgcaatca caatgtctag atcaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnncatatt taaatatcgg tcgtaatgcc acccctgcag aagtagccgc gtgggatatt
     9661 gatgtgcgcc cagactttaa aggactcccc gctggatctg gctcggtagc caaaggccaa
     9721 gatctttggg agaagcaatg cgctagttgc cacgggacct ttggtgagtc caatgaagta
     9781 tttacaccca tcgcgggtgg aactactaag gatgatatta agaccggtaa ggttgcatcc
     9841 cttatgagcg ataagcaacc acaacgcacc acgctgatga aggttgccac gatctccacc
     9901 ctatgggatt acatttatcg tgcaatgcct tggaatgcac cgcgctcatt gtcggccgat
     9961 gatacctatg cactggtggc attcattctt aatctgggtg aagtggtgcc cgatgacttt
    10021 gtgctctcca ataaaaatat cgctgccaca caagcactca tgcccaaccg aaatggcatg
    10081 acccaaaaac atggcttatg gaccgtgaag gctgttgggg atgtaaaaaa tactgcttgt
    10141 atgagtaact gcgcccaaca cgttgcgatt ggctcggtct taccagacta tgcacgtaat
    10201 gcgcataaca atttggctga acaaaatcgg gagtatggtc cttatcgggg cgttgatacc
    10261 accaagcctc cgttggccgc attaccagca ggcggaatga tgatggtgtc aacggcaagt
    10321 accgctccga aagtagcggg tccagcagat ctatttaaga aagagaactg ttctgcttgc
    10381 catgcgcccg cagcgaaatt agtgggccca tcgattgcgg atgttgcgaa taagtacaag
    10441 ggcgttgcaa cagcacaagc aatgcttgag aagaaagtaa aggcaggcgg cgccggtgta
    10501 tggggtgcga tacccatgcc agcacaaggg caactatcgg atgccgataa caaggtcttg
    10561 gtgcagtgga tgctgaatgg cgggaagtaa atgtagcagg gttagtactt agaatagcgc
    10621 ttgtagacag gctttatagt tatacgttaa atattgtggt cgtagataaa aaggagtttt
    10681 tatggatcag caacgtcgtg atgtactgaa gtattcagcg gtctttggtt tgatggctac
    10741 tgccggtttg attagtccag cccaagcaca agagtggaat aaggccgcat ttgaaggcaa
    10801 gagcctagat gatgtgttca aagtactggg tgctggtaag ccagagagct catcggcaat
    10861 taccatgatt gctcccgata ttgctgagaa cggcgctgtc gttcctgttg gcatgaacac
    10921 cacactgaag gcacagcaaa tggctatctt ggttgataaa aatccaagcg ccttagcagc
    10981 ccagttcttt attcctgctg gcacagaacc atttgtgacc acccgtatca agatgggtca
    11041 aacctccaat gtgtatgcgc tggttaaagc ggataacaag tggagcgtgg ctgtgaaaga
    11101 ggtcaaagtt actttgggtg gttgtggcgg ttaagtagcc caagtacaca cttatatata
    11161 gaacacattt agagaggaaa caacatgtca gatccgatgc gcgtacgagc aaccgagagc
    11221 ggtggcgtgg ttgatgtaaa gattttgatg aaacacgata tggaaaccgg tcagcgtaaa
    11281 gacgcttctg gtaaagcaat tccggcgtgg tttatcagca ccgtgaccgc aaaagcnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnng cgaagggtga caagatcacc gtatcgtggg tcgataacaa
    11461 aggcggaaaa cgcaccgacg aagcaactgc gtcctagttg aatacgttta ctccataaca
    11521 agaagaggga taccatgaga caacattgga tatatggcgc tgtaattgct gcaagcgctg
    11581 tgctattgca ggcctgtact acgcctgccg aagcgcctaa gaaagcttct gcgccagtac
    11641 ctgcagctcc tgctgcacct gcaggtgcac caaccgctga aatagaaaag tatcgcgcga
    11701 tgattgcgga tggcaatccc tctgatttat atgaaatggc tggtgaagag ctctggaaga
    11761 aaaaaatggg tcccaagaac gccagtcttg agaaatgtga tttaggtatg ggccctggtg
    11821 tggttaaggg agcctacgca gtacttccga agtactttaa agataccaat aaagtgcaag
    11881 accttgagtc acgcattgtg acctgcatgg agcgcttaca aggatttgat tccaaagaga
    11941 tcatcaaagg tacttttggt aagggtcaac gtaaagatgt ggaagcgatt gtggcttatg
    12001 tggttgccaa ctcaaaaggc atgaccatca atgtttcgac caagcatccc aaagagaaag
    12061 aaatgtatga gcttggtaag aaagccttct tcttnnattc gtttacaaga tctgcctaat
    12121 ttaacccagc aaaagggcgc tgcattaggg tggggcgcat ggcctgcata ccgagtatcg
    12181 agtggcacat tctggaccat gcaataccgt ttgaatgatt gctatcgtca gcaacgattc
    12241 cctgagccaa tttatatctc cgacgatacg attgcccttt caatgtatat ggctgcaact
    12301 gccaacggtg gcaagatgga agcccctggt ttgaagcgct aatggctgga gaaaaataat
    12361 atgaataata gatatctaat aacagcagta accttgcttg ctttgccaat gggctctgcc
    12421 ttggccgatg ctaagtttga caaaatgatg aaggatagtt tccaggcaaa tggtattgca
    12481 acgcttgatc gtttaaatca agatgctgca caaaagtatt gctctgaccc agctaccctc
    12541 gcagggaagg gtgaccccaa gcgtgcggaa gcaatccaga aagccaacat ggctacgatc
    12601 aagcaaccct ctgatggtaa atacattggt gactggaaat nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnntac aactgccacc aaattaataa aacggagttg tcttacggca
    12721 acatcggccc aagtttatgg aactacggta agttgcgcgg ttatagccaa gacctaataa
    12781 cgtatacatg gaaccggatt aacaactcta aagcatataa cgtctgtagc aatatgccgc
    12841 gttttggtca ctttaatctc ttgaccgaaa agcaaatgca agacgtgatg gctcttttgt
    12901 tagatccaaa ttcgccaatt aatcagtaat cacttaattt aggaagcgat atgtctttca
    12961 gtagacggga gtttctgcag gcccttgcag ttgcatcggc cggtggaatg agcttacagt
    13021 ctaatttctc actggctcaa agtgctgcac aaaagtttta tgacctacca aaatttggta
    13081 atgtccattt gttgcatttc acagactgtc atgcacaact catgccgatc tacttcaggg
    13141 agccgaatgt taatttaggt attggttccc aagacgggaa agtgcctcac ctagttggcg
    13201 aataccttct caaggcctat ggcattaagc caggcacgcg tgatgcccac gcatttacct
    13261 atctcgactt tgcagcagcc gccaaaacct acggcaaggt tggtggcttt gcacatctca
    13321 caaccctgat taagcaagtt aaagagagtc gtcccggtgc gctattactc gatggtggtg
    13381 atacttggca gggctcaggc actgccttat ggaccaatgg ccaagatatg gtcgatgcgt
    13441 gtcttgccat gggcgttgat gtgatgaccc ctcactggga gatgaccctc ggtgagaaac
    13501 gcgtgatgga gatcgtggaa aaagatttcc ggggcaaagt gtcgtttatt gctcaaaata
    13561 ttaagactaa tgattttggt gatgccgtgt ttgctcccta catcatgaag accatgaatg
    13621 gtgtacaggt cgcaattgtg ggccaagcat tcccctacac acccattgca aatccacgtt
    13681 acttcacacc caactggacc ttcgggattc aggaagagaa catgcaaaag accgtggatg
    13741 aagccagagc taagggtgcc aaggtggttg tcgtgctatc gcacaatggt atggacgttg
    13801 atctaaagat ggcttctcgg gttcgcggta ttgatgccat tatgggtggc catacccacg
    13861 acggtgtacc ggctccggtg aaggtaaaaa atccgggtgg cgttaccttg gttaccaatg
    13921 caggatccaa tggtaagttc ttgggtgtac tcgactttga tgtgaaggcg ggcaagatta
    13981 gcgacttccg ctacaaacta ttacccgtgt tctcgaacct attaccagcc gacgcaggta
    14041 tgagcgcatt aattaagaag atacgcgctc cgtatgaagc caagctcgcc gagaaattag
    14101 cagtcaccga tggtcttttg tatcgtcgcg gtaactttaa tgggagcttt gatcaagtca
    14161 tcttagatgg tttgatcaaa cagaagaacg ctgagatctc attctcacca gggttccgtt
    14221 ggggtacttc gctattgcca ggtgaggcta ttacgatgga gcacttactg gatcaaaccg
    14281 cgatcaccta tccatttaca acggtcacca atatgaccgg tgagaccatc aagaccgtgc
    14341 tcgaggatgt agcagataac ttatttaatc ctgacccgta ttaccagcag ggtggcgaca
    14401 tggtacgcgt tggaggattg caatatgcga ttgatcctgc cggcgcaacc ggtaagcgaa
    14461 tttctgagat gcgtctaaat ggcaaactga ttgaagcagg gaagacctac aaagtagcag
    14521 gatgggcacc agtgtctgag gaggccaagg cggccggcgg tgaagccatt tgggatttaa
    14581 tggctagaca cttaaaagaa gtgaagacca ttaaagccgt taaattaaac gagccgctta
    14641 tcaagggtgt ctctaaaaat ccaggtatgg ttgcaatcta actaaacagt cagggtcttc
    14701 tatgaacgca ttcttacgct ccatcctctc gtctttactt gtaatgagta ccttaggtct
    14761 tggatcaggc cttgccttgg ccgactctac taaggtggtc tatcacattg atgatgcggc
    14821 taatcaagga ttaaaaggct tgcgtaatat ccgaaatcac ttggatgtct caccccaaac
    14881 aaagattatc gttgtgacgc atgccaatgg tgtggatatc ttgatggatg gtgctaagga
    14941 cgcaaagagt ggaaccgatt acgctccctt agtaggcgcc ttgaaatcgc gcggggtgcg
    15001 ctttgaggtc tgtgaaatca ccctcagaaa tcgcagcctt aaaaaggagc aatttaccct
    15061 cgatgcggag ttcaccccct caggagtcgt gcgcattgca gacctgcaat ataaggataa
    15121 atacgcctat ataaggcctt agttttgccc tttacaaatc ttgtacgcta ctttttgaat
    15181 aactcagcat gtgtgccaat tcgggcgagt cttaattcat taccgctaat cttataaatt
    15241 aatacacaat catttgtgat gtgacaatcc caataatgac tggtatcacc ttttaacggg
    15301 tggttacagt attttggcgg taggggatga ttatttaaga gtaagcccat tacctttaaa
    15361 tattcaacct ggttgaattt ggggctttgt actatcttct taaaatcttt accaaattgt
    15421 gtgcttggaa ccggtaacag cattacttcg tgctgagccc cttatgcatt gcctctaggg
    15481 atttatattt ctttatcttt gttttggatt taggtgcctc gagctctttg atagcagctg
    15541 tagttttgga attaggtgcg cgtaattcaa atggaatagc accatcatct gccacacgac
    15601 gtaaaaatag gcgaatagcg tccgatatat ttaagcccat tgcttcaata acttcagagg
    15661 cagctgtttt ggtctttgca tcaattcggc aacggacgtc ggtagtgagc atgatgaacc
    15721 ctatatatgg atagcgtcat tgtagctaca tattaactac aagtcaaaac cgactattta
    15781 tgctggcggt agggcgactt agctccaaaa ggcttatttc taaaggtgag caattgaaaa
    15841 cagggctctt aataacctgt acttataaag gtatttctaa taaatctaac ttttaatggc
    15901 taaatctctg atttatcagt actttctata aaaatctgcc ataaaattat ggattcctaa
    15961 attccataag cattagatat gaaaaaagta gcactcttac tgattctcgg ctcggccatt
    16021 ttgtctcagc ccattgaggc tgcccccaat ttaaagaatg gcaaagaaat cgaagataac
    16081 aaatgctaca aatgccacgc ggataaatct ggctttgggg atggcagctt gatctatacc
    16141 cgcaaagatc gtcgtgtgca aaatcttgaa cgtctaaaag tgatggtggc tcgctgtaat
    16201 actgagttac gacttgatct atttcctgag gatgaggctg acgtcaccgc ctatctcaat
    16261 cagcagtact ataaatttaa gccctaattt cttatgaacg atctaacacc agagcaaatt
    16321 agttctctgg gcagtcaagc tttattgatt accctgttta ttacaacaat cctaggcatc
    16381 attatgcaaa agactagctt ttgcactctt ggtgctgtgt cggatggcat tctgatggaa
    16441 gactggtcca gaatgcgtca atggtgcttg gccattggca tcgcaatttt gggtgtagct
    16501 ttgatgtcgc acctaggatg gattgatgtc agtaaatcgt tttacaccgg caatcgtgtg
    16561 ctttatctct caactttgat tgggagcaca ttatttggta tcggcatggt tttggcatct
    16621 ggctgcggca gtaagaccct cattcggatt ggtggcggca acctgaagtc gatcgtggtc
    16681 ttcatcgtct taggacttgt tgcctatatg accatgcgcg gctttctagg gatcatgaaa
    16741 gcaaacacca ttgataaggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgatcttgg cgctcatcat
    16861 tggtggcgca tttataggct ttgctgttct caaaaaatca ttctggaatg ccgaaaacct
    16921 actcgctggt attggagtgg gtgtcggtat tacagcgatc tggtgggtct cgggacacta
    16981 tgcccacctc acagaggacc caaacacttt gcaagaagcc ttcttggtaa ctaattctgg
    17041 acggatggag agtctctctt ttgttgcccc ttatgcattc gccttagatt ggatgatgtt
    17101 cacgagcgat aagtcgaagg ttttaaccat tggcattgtg gctgtgattg gcatgatcct
    17161 gggttctgca attagctcca tccttagcaa aaactttcgc tgggagtcat tccgtggggt
    17221 agaagatacc gctaatcatt tggtgggtgc ggccttaatg ggctttgggg gtgtgaccgc
    17281 ggttggttgt acggttggac agggcttgag tggtctttct accttggcac tgaactcctt
    17341 tattgccttt cctggattta tcttgggagc ctacattgga cttcagtacc tacaatggcg
    17401 ccttagcccc aagccttgtt aggccttaaa ataagacgat gaccattgat tggacctcat
    17461 tctctccagg acccgcccta gtaggcggga ttttgttggg gattgctgca gctctctatg
    17521 ttctctttaa tgggcgcatt ctgggaatca gcggtattgt gggcggacta ttgcaggcaa
    17581 aaaaatccga ttggctctgg cggcttagtt ttacccttgg cctattaact gcagctttgt
    17641 gggggaagta tgtctttgat atccaggtga agcctgttat tgatgcggat tacctgacgc
    17701 tcatcattgc gggcttatta gttggctttg gagcacgtta tggatctgga tgtaccagtg
    17761 gccatggtat ttgtggttta tctagactgt cacctcgttc cttgttggct actttgacct
    17821 ttatgggctg tggcttccta attgtttacg ttgttcgtca tcttnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnggggttc tatttggttt gggcctaatg atctctggca tgaccaaccc
    17941 cgccaagatc ttggcattct tagatctttc aggagcgtgg gatccatctc tgatctttgt
    18001 aatgggtggt gcagtattgg tcggcttgat tgcgttttat cttgctaaaa atcggaccca
    18061 gagtttcttg ggaggtgcga tgcatatgcc gacacggcgt gatatcgatc gtcccttgct
    18121 aattggtagt gcgatgtttg gacttggttg gggtctggca ggtttttgtc caggacccgc
    18181 attggtatct ctgggctctg gtgagttaaa ggccttagcc tttgtaattg ctatgcttgg
    18241 tgggatgttg ctatttgatt ttgtgggtca gaagaaataa tcaagaccta ttattttgtg
    18301 aggattgcat gaacgtacat atttcttgtc ataacccaaa ctttggaaca gctggccaaa
    18361 ttgagcccac tgatgtagct gagattgcac agcagggtta taaaagtatt attaataatc
    18421 gtccggatgg tgaagagggc ccggagcagc caagcaatgc atcgattgca gcaatggcta
    18481 aatctcaagg actggaatac gcttacttac cggtcgtgag tggtgcgatt acnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnntcttaat accaacttcc tgctaaagcg atcaaaatgg ctgccttact gaacgatctt
    18661 gagcgctcca ttcttaaaac agagttagcc aactgggctg tagtacctaa gcgggatgca
    18721 atcactcgca cttattcatt taaagacttc aagagtgcct ttgcctttat gacccagtgt
    18781 gctctgcatg ctgaggcgat gaaccaccac cctgagtggt ttaatgtctg gaatcgtgta
    18841 gaggtgacct tatctactca cgatgcgggt ggtctcactc agcgtgatat tgaactggca
    18901 cgtgcgatgg atcgctgtgc tgcacagcac ctcatctagt aaatagtatg cgtacatggc
    18961 atatgaaacg aaattgttcg tttacaccca agcaagtagg ctatttttat ctctcaatgc
    19021 tgatgttctc atcgctcatt gctacgtatt tcttggtaca aggcatttgg atgatcttgc
    19081 catttaccat cattgagctt actgcattag ggatcgcctt attaatctac gcacgccacg
    19141 cactcgatta tgagtcgatt gccatcgatg gtagttcggt taccgttgag aagaacattg
    19201 ggggcaagct cgagcgccat gagttcaaca ccaagtggtt gcaactcaag caagaagaag
    19261 acggtaaacg attaattcgg atagagcaaa gcgatcaaga gctccccatc ggtatctttg
    19321 taccagtaga cgccagagct caatttttca aggatctgcg ccagcaaatc cgctgcgagt
    19381 cttagggcac agggtaaact agatctttag cacccaagga acttagtggt atgcggattg
    19441 atataccaga cgataaaaaa ttagtgcacg aagccatcat cccattgcgt tggggcgata
    19501 tggatgcctt tgggcacatc aacaacaccc tgtactttcg gtatatggag caggggcgta
    19561 ttcattggat tgagtccttt ggatttggta ttcaacatgc tgagggcggg gccttaatcg
    19621 ctaatgcatt ctgtaatttc tattcacagg tatcgtaccc aggtgatctg atcatcaaaa
    19681 catatgttgg caaagtggga cgtacgagtg ttgatatcta taacnnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca tggcctgagc cctttcagaa
    19861 acatttgggt ttagcttgag ctcttggatt gttgaaccgg ggtctttaca tcaagacctg
    19921 agccgctttg actgcattat tgatgtacgt tcacccgcag agtttgcctt agatcacatt
    19981 cctggcgcaa tcaactgccc tgtactaagt aatgaagagc gtgcgcaagt aggcacgatc
    20041 tataaacaag aatccccatt tgctgccaag aaaattggtg ctgcattagt agccaagaac
    20101 attgccggcc acttgcaaga ccattttctt gatcaagagc gtgattggac cccattagtt
    20161 tattgctggc gagggggaga gcgcagcggc gcatttactc atattctgca acgcattggc
    20221 tggaaagcgc atcaactccg cggtggttac caggagttcc ggcgtcaagt tatcaccgat
    20281 ttagatcagt gggcggctca acttcagttc aaggtgatct gtggcatgac tggtagtggc
    20341 aagactagac ttttgcaagc actcaaatcg catgcccaag tattagacct agaagaactg
    20401 gccgctcatc gcggatcggt attgggtggc ttgcctcatc aagagcagcc aagccaaaaa
    20461 gcatttgaga ccggcttatg gaatgcctta cgtcattttg atcttcagaa agtgatctat
    20521 gtagagtcgg agagtaaaaa agtaggcggc ctacacatac cagatgcctt gatggagcag
    20581 attcggaata gtgagtgcct cgagattcag gctgagcgca gtgtgcgggt cgattgctta
    20641 atggaagaat acgcacactt cttagaagac aatcaggccc tcgaaaactt actgacttta
    20701 ttaacccagc gttatggcaa agaacaaatt agtcaatggc aagcactatc gcaagagaag
    20761 aagtttcaaa tactggtgga tgaactgcta gttcgtcact atgaccctgc gtataccgat
    20821 tcgatccaaa gaaacttccc gaactacaca gcaggaaaaa agcttgtgat t
//
LOCUS       DDPQ01000145            3855 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_7867,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000145 DDPQ01000000
VERSION     DDPQ01000145.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_7867"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    453..488
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2642..2719
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1020 a          745 c          977 g          999 t
ORIGIN      
        1 atgttcttac acaatggcaa attggttgga gacatttctg gggatgcctt tgcgaaagct
       61 ttttttggga tctggcttga tgagcgaacc agcgccccta aattacgctc ggcactgatt
      121 gccactcgat gcccgcccgc attaattgcc gcaaattgcc cgaatcctta acaatttttc
      181 tcttaggtgt taatcatcat gaatctcttg agccggctta aaagcatctt tgtagtatgt
      241 gtgatggtat tgggtgtggc atcgtgttct ggcccaacgg tgcagatgta tgcaaaagaa
      301 aagccccagt tagatttagc cacgtacttt aacggcgata ttgatgccta cgggatcttt
      361 acaaaccgca gtggtgaggt ggtgaaacgt tttaaggtgg tgatcaaggc gaagtgggaa
      421 accaaagatg gtaagcgtgt tggtgtgttg gannnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnngg gaaatattct gggactgcca gcgatgtgat tggcgaggca gtcggcgaaa
      541 tggcaggtaa tgcactcaat tggagatata ccctcgctct gccggtcgac ggcacaatct
      601 atcacgtcca gttcaatgat tggatgtatc tgatggacga taaggtcatg cttaataagg
      661 cagcgatgac taagtttgga atctaccttg gcgaagtgac cttggctttt tataagcgct
      721 agagactggc atcgtgacct tagtaatttc tagagaggtg tggcgcaaga tttggaattt
      781 tgttttattc caagccgcct ggtttgcttg catattagga gcagcagacc agcagatttg
      841 gatcgccttg agtgtctccg ctatttgcat tggcatttat ctgtggttgc acgccaatgc
      901 cgcatcggaa tacaaatttc tgctgaaggc ttttatctat ggcctagtag ttgataccct
      961 attgcttcaa ttgggttgga tccagtttga gtctcctttg ccctggattg ggatatcacc
     1021 gatttggatg tgggctcttt ggttggtttt tgccaccacc ctcaaagaat cgatgtcttg
     1081 gttacagggt aaatgggtct tgtcagccgt attaggggcg attgctggcc cactttgcta
     1141 cgaggcgggg gtccgcctag gtgcggctca gtggccaaac tccgatatac aggtctttgg
     1201 ccttatctac cttgcacttg catgggcgtg tgcaatgcca ttacttttct atttcagcca
     1261 aaaagatcat taatatctat ataaatcaaa tacttataag tttataggcg cttacttaca
     1321 aattaatatg tagttactga tgctgaaatg ttctagaggg gcttgcaaat agtactgttt
     1381 ttatatacag taaacataca gtaacgatta acagacctaa atacagcaaa acaccgtaca
     1441 gaatagaaaa ggacagccat gaacgacaag aaaaaatcag cctcatccga atttgaagga
     1501 atgagtggag acaagcaaaa agcattaacg gctgccttag cgcaaattga aaaacaattt
     1561 ggtaaaggct cgatcatgcg tctgggtgat gcagagatca atcaggacat acaggtggta
     1621 tcgagcggtt ctctcggttt agatattgca ttaggtgttg gcggcctagc acgcggacga
     1681 gtgattgaaa tctacggacc agaatcatcc ggtaaaacga cgctcacttt gcatgcagtt
     1741 gcagagatgc aaaaactggg tggcacttgt gcgttcatcg atgcagagca tgcattggat
     1801 gtgcaatatg cagccaaact cggcgtcgat gtcaataatt tattaatctc ccaaccagat
     1861 acgggtgaac aagcattgga aattgctgat gcgttagttc gctctggctc cattgatctc
     1921 attgtgattg actcagtggc tgcattagta ccgagggcag agattgaagg tgatatgggc
     1981 gactcattgc caggcttgca agctcgctta atgagtcagg ctctacgtaa attaaccggt
     2041 acgattaagc gaaccaattc tatggtgatc ttcatcaatc aaattcggat gaagattggc
     2101 gtgatgtttg ggtctccaga aacaactact ggcggtaatg ccctgaagtt ctacgccacg
     2161 atgcgattgg atatccgccg cattggcagt attaagaagg gcgatgaagt cgttggcaac
     2221 gaaacacgcg tgaaagtggt gaagaacaaa gtatcgccac cattccgcga agccattttt
     2281 gacatcatgt atggccaagg aatttcacgt gagggtgaaa tcattgatat gggcgtagaa
     2341 gccgatatcg tcgagaagtc gggtgcttgg tatagctatg gcggagagcg cattgggcaa
     2401 ggtaaagata atgcccgtga gtttttgaaa gaaaaccctg agatggctaa agagattgag
     2461 gcaaagatcc gtgagaagtt gggtgttaaa aacggtggct ccttgattag cgaaacactc
     2521 gatgatgagg agatggaatc tgcggacgct taatgcaggc tccaagtaaa tcatctaaac
     2581 aaagcccgag tctcaaagct cgggctttgc gtcttttatc tcgacgtgaa tacagtcgca
     2641 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnt atgggattcg acgcgtcgca gatgagctgc aaagagcagg
     2761 ggttgaaacg ggccttatta cccaattaac gggcgaactc aaagagtctg aatacgaccg
     2821 tgccaagtcc ttatggaccc gtaaatttgg tcaaattgcg attgaacaaa aggagcgggc
     2881 gcgtcagtac cgctttctta tttctaaggg atttagccct gaccttgtgg ccaaggtgat
     2941 tggcggacgc aaagcatcta tttagccgaa ttaaggcttg aaaatgttag acttatgggt
     3001 cttttgcagg cgttctaagc cccgttttgc cttgatttgg gtcaatagcc ctaaaagctt
     3061 catcagggaa aatagctttc acgctggcgt tgaaaactaa tcatttccag gagctgttat
     3121 gaagattcat gagtaccaag gcaaggaaat tctgcgccaa tttaatgtac ctgtgccaaa
     3181 tggtattccg gcgtttagcg tcgatgaagc cctcaaggcg gctgagaaat taggcggtcc
     3241 tgtctgggtt gtcaaagccc aaattcatgc aggtggtcgc ggtaagggcg gcggcgtgaa
     3301 gttagccaga agcatggaag aggtaaaaag ctatgcctct tcaatcttgg gaatgcaatt
     3361 aaagacccat caaacgggac ccgaaggaca aaaggttcgt cggctcttaa ttgaggatgg
     3421 tgctgatatt aaaaaagagt attaccttgg aatcttgacc gatcgcggta cccaaaaagt
     3481 ggtgctgatg gcttctagcg aaggcggcat ggatattgaa gaggttgctg caaaaacccc
     3541 tgaaaagatt attaaagtat ttgtggatcc actaattggt ttaagtgatg cacagtgcga
     3601 tcagttaagt aaagggattg gtgttcctga ggcatcccaa ggtaaggctc gtgaggtgtt
     3661 caagaatcta tataaaactt actgggatac cgacgcatct cttgtcgaga tcaatcccct
     3721 catcttagaa ggtaatggca atatcaaagc acttgatgcc aaatttaact ttgactcgaa
     3781 tgcgctgtat cgccatcctg agatcgttgc ctaccgtgat gaagacgagg aagatcctgc
     3841 ggagattgag gcatc
//
LOCUS       DDPQ01000146           18704 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_14129,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000146 DDPQ01000000
VERSION     DDPQ01000146.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 18704)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18704)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18704
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_14129"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    1757..1826
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3402..3494
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10593..10697
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4696 a         4114 c         4574 g         5044 t
ORIGIN      
        1 gagctagtcc cttgaagttt ccacaagcgc tgctgaggca gtgtgaccct ttggtattaa
       61 ggccgttggg ataggggtag cgttgaccct tcgtaaagcc cgaaggtggt gaaggacgtt
      121 gctctaaata agccgcttca acccactctt tatcgcttgg taggcgtttg ccaaaatact
      181 ggcaaatggc ttgggcttca aatgcattaa ggtgaacggc cggctcattg tctcccgcag
      241 ggacgccata aggtgtgcgc caagtccacc ccggtttttt tacaaatccc aactcatagg
      301 atgtgccgcc tcctgctttc tcagcagtgc tctgaaaacc agtagcgctg gcaaatcgtt
      361 tgacctcccc aatggtcatt tcatgttgat cccagagaac tcctttgatc tcaacagtgg
      421 ggatggctga tttggcagat tgcccatacg catggaatgc caatagcgat agagtaaata
      481 tgcaaagttt attaattgtg cgaatattca tagaattgta aaaaattgac aaataagtaa
      541 cctagccatt tttacctaat tcagtgcgac ttgcactatt gctattagag cgaattaagc
      601 atcatgaaaa aatccattct tcttctgcta agtacctgtt ttatagttca agtggcagtt
      661 gccaatccag aaacaacaaa ggctgctaaa gaagcgccaa agacagatgc caccatcaaa
      721 gctgaagtgc tctcagtctt ttttagccca cctgctgggg cagccaaggg cttgattcgt
      781 tatctcgatg gcgccaagaa gagtatccaa gtaatggctt acagttttac ctatgtagag
      841 attgcagagg cacttgcccg agccgccaaa cgtggggttg aggtacaggt tattcaggat
      901 ggaccaacgg cagccaataa ttttgatgtg atcccaatat tattaaaggg cggtgttgca
      961 cttcgcggta ataaagccca ccgcatcttt cacaataagg tgatgattat tgatggcgat
     1021 attgtggtga ccgggagcta caactttacg aatagtgcag aaaagagtaa tgcggagaat
     1081 atgattgtgt tgcgctccgc tcctttggcg caacagtact ataaaaactt catctcccat
     1141 tgggagcgca gctatcccat tacttctgcg ccagagcgta aaacagctaa acccaattaa
     1201 tctggttcag tcacaaatcc taattttgtg aggccagcac gccgagaggc tgctagcact
     1261 tgagcaacag aatcgtagcg cacagattta tcagcccgta aattaatttc gggttgggga
     1321 tctttcttag ccgctttatc ggcataagta ctgaaggtgg ctatgtcaat gggggtgctg
     1381 ttccaaaaga tttgcccttt ggcatcaatg ctcaattgca cagattctgg ctttacttca
     1441 ttgcgcacac tgtttgcttt gggcagttct actttgactg cctgctgaat cactggcaaa
     1501 gtaatgatga aaataatcag cagtaccaac atgacatcca ccattggagt catgttgatc
     1561 tcggccatta aaccatcttc gttttgatca ttcgagagat tgctaaaaga catgattact
     1621 cctgatcggg aacgcgagca cccgtgacaa agtaggcgag tagatcgttg gcaaatcgat
     1681 tgagatctgc catcactaat ttattgctgc gattgattgc gttgtagccc aagaccgcag
     1741 ggatcgcaac cgcaagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnggcg cggtcgcacc ggttgacgcc aaaaaggtta
     1861 accccttctg gagttgacct gctgatgcat caattccgtt cttgagacta cgtgccatcc
     1921 attctgaata gttcagggtt tgtaagagtt cgcgatggtt tgagcgttga ttgagatgat
     1981 gggcagaggc ctcattagca gttttagcta ctttgtaaaa agggttgagt gcatggttgc
     2041 taagtgaggc aagaccctca tcaaacgagc gtgctctcca aaatccatct agttcatttt
     2101 tgagacgttt gatattgcgc aatccccaaa tacggctgat taagattacc cacgtgacca
     2161 ctgataaaaa cagaagaacg agtgcaacaa agtgagttac gaagtctccc tgggtccaga
     2221 ggtggtgaag gccaaatgca ttgttcatat tagggactct ttaggttaaa tttaatcagt
     2281 agattagtgg agattttagt aggactgcca ttaatcaggt agggcttaaa gcgatagcgt
     2341 ttaccaattt ccattgcggc cctgtctagc cttgggtaag agcttgattg caataggcgg
     2401 gcatcttcaa caacccctgt ctcgtcaatg ataagacgca caacaacctc gccttgctcg
     2461 ccagcacgtt tagagaatga ggggtagtag ggatctgcat caggttggta aaccacaatc
     2521 aatttaccaa tatccgtttg aatcggagtc ccactaactc cgccaccccc tgttgagggc
     2581 gcaacggtcg ctgattgagt actttcactc gattttgcag aaggggttgg aggtgctggt
     2641 gttgggctac tttgtgtggg actgggcgca ggtgttggtg taggcgttgg agttttctct
     2701 gccttcttta cctcaggttt gggagttggt ttcggagttg gtttaggagc ttctaccggt
     2761 attttggggg cggggggcgc actttgctta gggggcgaag gactatccgg tggaagcaag
     2821 ttcgccagga cccgttcatc attcaaataa ttaggaggct ttggggtaag tccaaaatta
     2881 aatggcacga taaaagccaa atgagccaga accacgaagg cgatgatgat gcgctcttcc
     2941 ttggaaaaag gaaggtgtaa acgcgattgg gtcatgctat tcatcaatta gagctttaat
     3001 gtctcggtat aaatggtgta ctgcgataaa tcacgaagca gtaagcgttg tgccatcgtc
     3061 tgtaatgtat ccgcattggc cgtgctgagc aagcttaacg acggggcatc tatgtttgga
     3121 ttagcgctga tcccttgttg atccatttgc cgcacaagtt gacgcacaac agcatcgctt
     3181 gtatcgatta gttccatcga gtcgcctacg atgtctcgaa tgatgttctt gagaaagggg
     3241 tagtgagtgc atccaagaac caaggtgtca gcgccgcgct cttgaatggg gcctaaatgg
     3301 gagcgtaaga gggcgtgcat ctctggggtc tcaatctgac ctgcttcgat caaaggtacc
     3361 aaacccgcac cagcctgttt aatgaattgg caatcttgcg gnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnatgatt gggatatcta gtgtgctacg gattgcagca attgcttccg
     3541 ctgtagcagt gttacatgcc accacgatgg cactgcaatt ttgttctaat aaatagcgac
     3601 agatttgtaa gctgcgttcc gcaatccact ctggcgacct attgccgtaa ggagcattcg
     3661 cggaatccgc aaaatagatg tagttgtggt ggggtaactg ctggagtgcc tcatccaaaa
     3721 tggataagcc gcccacacca gagtcaaaga caccgattgt taccacggtc aaccgttcaa
     3781 ggaattgcag tgcatctaag ccacgctgac tggtatcttg ccaatcttga gttgccattc
     3841 acgcggtcct gtttgatgca tggaggtacc actcgaatcg actgccacgg tgactggcat
     3901 atcttcgacg tcaaactcat agatggcttc cataccaagg tcttggaagg caaggacttt
     3961 ggcatgctta atcgctttgg atactaagta ggcggcacca cccacagcca tcagataagc
     4021 cgagcgatgt ttcttgatcg actcaatcgc aaccggaccg cgctctgctt tgccaatcat
     4081 ggcgagtagg cccgtttgcc ccagcatggt ttcagtgaac ttatccatgc gcgtggaggt
     4141 tgttgggcct gccggaccca caacttcatt gcggactgga tcaacaggac ccacataata
     4201 gatcacacga tttttgaagc taactggtag agcctcacct ttggacagca tatcggcaat
     4261 tcgtttatga gcggcatcac gacccgtcag aatcttgcca ttgagtaata aggtttgacc
     4321 gggtttccag ctcgccactt cttccgcact taagttgttt acatccacgc gtttggattt
     4381 cttggtatcg gcttgccatg agactgctgg ccaatcacta agagaaggtg ctttgaagtg
     4441 agcagggccg ctgccatcta aatgaaaatg aatatggcgg gttgctgcac agttcggaat
     4501 catcgccact ggtaatgacg cagcatgtgt tgggtaatcc atgatcttga catcgagaac
     4561 ggttgcaagg ccacctaagc cttgcgcacc aatcccgagt tgattgacct tctcataaat
     4621 ttctaggcgt aactcctcga tacgattctt gggtccgcgt gcaatgagtt cctgaatatc
     4681 aatcggtgcc atcaaggact ctttcgcaag gagtgcagcc ttctcaggcg tgccaccaat
     4741 cccaatacca agaatgccgg gagggcacca accggccccc atggttggca ctgtctttag
     4801 gacccaatcc acaatcgaat ccgatggatt gagcatgatc attttggctt tgttctcaga
     4861 gccaccccct ttggctgcgc aaatgacctc tagatcatcg ccaggaacaa tttcataatg
     4921 aatgactgct ggagtgttat cgcctgagtt cttgcgcttg cccgcagggt ctaataaaac
     4981 agaaccacgt aatgtgttct caggattcag ataagcacgc cgcacacctt cattgaccat
     5041 atcggcaacg ctcatcgtgg cgccactcca attgatattc atgccaacct ttagaaagac
     5101 cacagcaatg cccgtgtcct gacaaattgg ccgtttaccc tcggcgcaca ttctgctatt
     5161 ggtcagaatt tgggcaattg agtctttcgc agcttcgcct tgctcgagtt cataagcacg
     5221 gcccatcgcc tgaatgaaat cccgaggatg gtagtaggaa ataaactgaa aggcatctgc
     5281 aatgctttgt atgaggtcgt cttggcgaat ggttggcata gggttatttc aataagtgga
     5341 atgaatatgg cattttaagg gtttgccata gggcaaatca ggacttaatt cacttatcgt
     5401 catatgaaat aatcaaacaa ttattctttt gttgtaacta taagtactta gagagggtgt
     5461 tattcaaatg gaaccactga tgaacgagag ccgggttttc aatccgccca ccgattttgt
     5521 aaaaaatgct gccatttctg gcatggatgc ctacaacaaa ttatgcgcag aagccgaaaa
     5581 agattacgaa ggattttggg cgcgcttagc caaagaacat gtttattgga agcagccttt
     5641 tacaaaaaca ctggatgagt cgaaggcacc gttctataaa tggtttgaag atggcaccac
     5701 caatgcttct tataactgcc tagatcgtaa tctagaaatt ggtaatgcca acaagaccgc
     5761 catcattttt gagtctgatg atggtaaaga gaccaaggtc acataccaag aactgcatga
     5821 tcgtgtttgt caattagcga atgccttgcg taagcgcggt attaaaaagg gtgaccgcgt
     5881 cattatttat atgccgatgt cggtcgaggg tgtgatggcg atgcaagcct gtgctcggat
     5941 tggcgcaaca cactcggtcg tgtttggtgg attctctgcg aagtctttgc aagagcgcat
     6001 cattgatgcg ggcgcagttg ccgtcattac tgccgatgag caaatgcgcg gtggtaaggc
     6061 cttgcccttg aagaccattg ttgatgaggc tttggcattg ggtggttgcg agatcattaa
     6121 gaacgtcatt gtctacaagc gaaccggtgg caatattcca tttaaggccg gcttggattc
     6181 ttggatgcat gagattactg cgagcgaggc gaagacctgt gatcccgagt gggtgagtgc
     6241 tgagcacccg ctatttattt tgtatacctc aggttcaact ggtaagccaa agggcgtaca
     6301 acattccacg ggtggatata tcttgtgggc cattcttact atgaagtgga cctttgatat
     6361 caagccaagc gatatcttct ggtgtactgc ggatattggt tgggtaacag ggcactcgta
     6421 tattgcctat ggtcctctgg cagtgggcgg aacacaaatt gtgtttgagg gtgtacctac
     6481 attccccaat gccggacgtt tttgggagat gatccaaaaa cacaaggcta gcattttcta
     6541 tacagcaccc acagcgattc gttctttgat aaaggcgtcc aataccgatc ccaatgtgca
     6601 tcccaaaaat tatgatctga gctcactgcg tttattaggt tctgtaggtg agccgattaa
     6661 tccagaggcg tggatgtggt actacgagaa catcggaggc tcacgttgcc cgattgccga
     6721 tacattctgg cagactgaaa caggtggtca catgattagc cccttgccag gcgccacccc
     6781 catggtccca ggctcttgta ctttgcctct ccctggcatc atgaccgcga ttgtggatga
     6841 gatcggcaat gacatgccta atggtcaagg gggcatctta gtagtcaagc gtccatggcc
     6901 atcgatgatc cgcaccattt ggggtgaccc cgatcgnnnn nnnngatggt gcaattcgga
     6961 ataaagatac gggttacttc acgatcacgg gtcgcatcga cgacgtctta aacgtctcgg
     7021 gccaccgtat gggtacgatg gaaattgaat cgaccttggt tgctaaccct ctggttgcag
     7081 aagctgcggt agtagggcgc ccggatgaga tgaccggcga agcgatttgt gccttcgtcg
     7141 ttcttaaagg aaagcggcct gagggcgatg atgccaagaa gattgcggtt gaattacgta
     7201 actgggttgc aaaagagatc ggcccaattg caaaaccaaa agatattcgt tttggagata
     7261 acttgcctaa gacccgctct ggcaagatca tgcggcgttt attacgtgtg attgcaaaag
     7321 gggaagagat cacacaagat acctctaccc tagagaaccc ggcgatctta gaccagctaa
     7381 aacagcctct ttaaccatgc gattaggctc gaagggctta tcaagctctt cgaaccgtat
     7441 aatgatcggt ccggagaggt ggatgagcgg tttaagtcac acgcctggaa agcgtgcgta
     7501 gggttaaacc taccgcgggt tcgaatcccg ccctctccgc cattagggta tttttgtaat
     7561 tactttttaa caagggaccc tttaagaggc ttcatcaatg aaaagttcaa tttataccca
     7621 aggtcttgat aagaactcag caaattatgt agcgcagtca cccctcactt ttattgagcg
     7681 tgcggctacg gtgtatccca atcgacccgc agtgatccat ggcgatattc aaagaaattg
     7741 ggcgcagacc tacgagcgct gccgacaatt tgcatccgca cttacgcaac atggcatcaa
     7801 acgatttgat accgtcgcag taatgctccc aaatattcct gcaatggtcg aggcgcactt
     7861 tgcagttcct atgacgggcg cagttctctg cgcattaaat acacgtttgg atgccgcagc
     7921 tattgccttt atgttggagc atggtgaggc gaaagttgta ctcatagatc cggaattttc
     7981 tggggtaatg agcgctgctt tggcaatctt gcgcgataag aaattaactc ttccatacgt
     8041 tgtggatgtg agtgatgttt tatatcaagg ctcagcaaaa ccgattggtg caatcgagta
     8101 tgagcaattc atcgctttgg gtgattcaaa ctttccatat caattacccg aagatgagtg
     8161 ggattcaatt tgcttaaatt acacctcggg tactactggt aatcctaagg gagtggtgta
     8221 ttcccatcgt ggtgcatata ccaatgcaat ctccaatatc ttggaatggg atatgcctcg
     8281 ccaccccact tatttatggg tcttgccact gtttcattgc aatggatggt gttttccatg
     8341 gactgttgct gcgcgcgcag gtgtaaatgt ttgtttgcgc aaagttgacg cccaaaatat
     8401 ctttgaagca attcggaaac atcatgtaac ccattactgt ggagcgccga ttgtgcataa
     8461 catgttgatt aatgcgcccg atgagatgaa gcagggtatc aaccataccg tgagcgcaat
     8521 ggtggcaggc gctgctcccc cggcagcaat gattgaggga atggagaaga tgggttttga
     8581 tattacccat gtatatggtt tgaccgaaac ctatggccct gcagccgtct gcgtaaagca
     8641 tgaggattgg tctgatgtcg atgtaggcga gcgcgcccgt ttgaatgcaa gacagggcgt
     8701 gcgctatcac cttcaacatg ccgcagcagt gattgacccc gaaaccatgc agcccgttcc
     8761 tgctgatggc gagaccatgg gtgaaattat gttccagggg aatatcgcta tgaagggtta
     8821 cttaaagaat gagaaagcaa cgcaagaagc atttgcgaac ggctggtttc attcaggcga
     8881 tcttgcggtt atgtttcctg atggctatat caagattaaa gatcgcagca aagacatcat
     8941 catctcagga ggagaaaata tctcgagtat tgaggtggag gatgtgctct atcgtcaccc
     9001 agcagtattg gctgcggcag tggtggctaa acctgatccg aagtggggcg agaccccctg
     9061 tgcttttatt gaactcaaag cgggtgcaaa tccaacgcca caagagattg tggagcattg
     9121 caaaaagcat ttagctggat tcaagattcc acgtgccgtg gtctttggtg aaattcctaa
     9181 aacggccaca gggaagattc agaaatttga gcttcgtaaa cgcgctggct ccgcttctgc
     9241 tattgacgta taaatatgcc agccgattac cgtgcccccc tcgcagacat gcaatttatt
     9301 ttgcatgaac tgattccttt ctccactatt aatagtctgc cgccatatca agaagtaagc
     9361 gatgatttgg tcaatacgat tttggaagaa gcctctaaat ttgcaggaga ggtcttgggt
     9421 cccttgaact ggacggggga ccaagaaggc tgttcatact cgaaaggggc ggtgaagacc
     9481 ccacagggtt ttaaagctgc ttatcaacaa tatattgagg cgggttggat ttcattggct
     9541 gctaatgctg attttggcgg acaagggctc cccattactc tagccacacc cgtcaatgag
     9601 atgtggcact cagccaatat ggcattcatg ttgtgcccca tgttaactgc cggtgcgatt
     9661 gaagcaatag agcatcacgc ttcttctgat cagcaagcgc tctatttacc accccttgtt
     9721 tctggccaat gggctggaac catgaacttg accgagccct ctgcgggatc tgatctctcc
     9781 gcggtgagtt caaaagcgat tccaaacggt gatcactatt taattagtgg taccaagatc
     9841 tttattactt acggcgagca tgactttact gaaaacatta ttcatttagt acttgcacga
     9901 ctgcccgacg cgcctccagg ggtcaagggt atatcgctgt tcattgttcc taagttttta
     9961 gtgaataccg atggcagttt gggcaaacgt aatgatgtgc aatgcgtctc gattgaacat
    10021 aaattaggga ttcatgcgag cccgacggcg gtaatgtctt tcggtgagac tggtggtgca
    10081 attggctatc tggttggtga agccaatcgt ggactggagt atatgtttac catgatgaac
    10141 aatgcgcgct tagcggttgg tttagaaggc gttgctattg ccgaacgcgc ttatcagcaa
    10201 gcagcgcaat atgcacgaga gcgcgtgcag ggtaaggtcc cacaaaaaac agataagctt
    10261 ccaatcttgc accacgctga tgtacagcgc atgttgatgc tcatgaagac gcagacagaa
    10321 gccatgcggg cgctcgccta tattgccgca gcacaatccg atctcgcaca aaagcatcca
    10381 gatgcgtctg ttcgtgatgc cagtcaaaag cgcgtggatt tgctgacccc gattgtgaag
    10441 gcatggtcta ccgagcaggc gattgagatt gcctcactcg gagtacaggt gcatggaggt
    10501 atgggctaca ttgaagagac cggtgccgct caacatctac gagatgcacg catcaccacg
    10561 atttatgagg gcaccaccgg aatacaggca ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnngct ttagatcagg cagtggattg gttattgaag atgcatacca
    10741 aaaatcccca agctacatta gcaggtgctt atccactcct taaatgcttt ggtattgtgt
    10801 gcggtggatg gcttttggcc aaatctgcat tgattgcttc ccatagactt caaaaaaatt
    10861 caggtgatcg ctttgcccaa caaaagattg gaagcgctat cttttatacc catgaagttc
    10921 tagcgcaggc gcctgggctt gttagcacca tttgctctgg agagtctgtt gctaaggcga
    10981 tgaatgatag cttggctgat ttgttgaact aagaaatacc aacacaacgc ataccaattt
    11041 ctgctaacag gcctgctttc tggccatttt ctcttgcatc gcttgcactg gcgttaccat
    11101 ctaccgcccg ctgcgcaacc ccatgtaaga tggctgcaat acgaaagaag ttatacgcca
    11161 tataaaagtt ccaatcagag tttaccgttc tgccggagtt tgtttcatac cactctatgt
    11221 attctttttc agtaggaatt ccgagcttca ccaaatcaag tccaccaata ccgcgccata
    11281 gtgatggggg gattcgccaa gccatgcatt gataagaaaa atctgccatg gggtggccta
    11341 gggttgagag ttcccaatca agcaccccga tcactttatt ttctttgtgg tcaaagacca
    11401 tgttatctaa acgaaagtca ccatgcacaa gggtcgtttc atcatcagcg ggaatgtgat
    11461 gaggtaacca ttcgattagt tttaaaagtg cttctggaat gggaatagtg ctttcgttca
    11521 cttggcgtga ccaacgacct acttggcgac taaagtaatt tccgggttta ccaaaacttt
    11581 ctaaaccgat tgcttggtaa tccactttat gaatcgcagc aatcactcga ttcatctcat
    11641 ggtagattgc acgtcgatca aattggctga gatggggcaa ggattggtca ttgaatacgc
    11701 gtccgtctaa gaaggacatg agaaagaagg gtgtgccgat tgccgactcg tcctcacagt
    11761 aagacagcat cttgggtact gggacctctg tcattgagag tgcttgcatc acgcggtatt
    11821 ctcggtcaat cgcatgggcc gagggtaata acttgccagt tggcttctta cgcagcaccg
    11881 catgttgatt gccggaactg attttgtatg ttgggttgga ttgaccgcct accaatgggc
    11941 tcacttctag accttcagta tcccagccta aatctttaag atatagctcg agcgcttttg
    12001 tattaaacga gatgctcacg caatgctcta taaagaacta acgagatgtg cgccatcgac
    12061 cgggatggtg ctgccagaga ttgctttgcc agcatcggat gccaagagca gaatgggtcc
    12121 atccaaatca ctcagttcac taaagcgccg actcggaatc cgtagacgca atttttctcc
    12181 aagctcgctt tgtaaaaact ctcgattcaa atcggtcacc acatatcccg gcaggatagc
    12241 gttcactcgg atttgataac gagccagctc taaagccatc gtcttagtca gttgaatgac
    12301 gcctgctttg gaaatggcat acggggcaac tccattgatt tggcgctcgc ctaatatcga
    12361 tgccatattg ataatcgatc cgctggtctt gcttgccacc atgtggcgtg ctgcttcggt
    12421 cgctactaac catactccct tgagattggt gtcaatcact tgatcccaat ctgtctcggt
    12481 ttgctctaga aggcttttgg tcaccgagat accagcgtta ttaatcacaa tactgggtgt
    12541 gccccattca gaaatggcat caaagcaggc tttgacgctt tcaatattgg tcacatcgag
    12601 ggggaaggct ttcgctaaac cacctttttg ttcgattgct ttgacaatag actcgagttg
    12661 atcgatgcgt ctagaagcaa gtgccacttt tgcacccatg ctggccaata gttcactaca
    12721 gtgacgtcca attccgctgg aggcgccagt gaccaaagca agttgaccat ctagacgaaa
    12781 gcgctcagca gggctcatgc cttttgatcc aaaagttttc tggccatact ccaacgatgt
    12841 acttcactgg gaccatcgta gatacggaaa gcacgcatat cggtaaagat acgcattacc
    12901 gcagtttcgc ccgtcacacc ttgtccaccc ataatttgaa cacagcgatc cacgactcgc
    12961 cattcagctt ctgagcaaat tactttggca cggctggatt cataattaca tttctcacct
    13021 tgatcaagca accatgcggt atgcaaaata tgcaagcgtg cggtttgaag atccatgtca
    13081 ttgtcagcca acataaatcc aacaccttca tgatcgccga gccgttgacc aaaagcctgt
    13141 cgcttttgtg catatgctag tgcaatatcg tgcgcacgcc gggcttgacc tagccaacgc
    13201 atgcaatggg tcaatcgtgc gggggcaaga cgcacttgtg cataccgaaa gcctttaccg
    13261 acttcaccca aaatttgatc ttcgggaaca cgtaaattat taaaacccaa cacactatgc
    13321 ccgcccgcaa aacactgatc catcgaatcc atattgcgtt caatctcaat tccaggagca
    13381 tccatgtcgg ataaaaacat ggtcgcttgg ccatcttcgg tacgtgccat gatgatcaca
    13441 tagtcagagc catcggcacc agtgataaac catttacgac cattgatcac gtagtctttg
    13501 ccatctttga ttgctgtcgt tgttagcatc gatggatcag atccggcacc aggcgcaggt
    13561 tcagtcattg caaagcagga acgtaactta ccttcaattt gaggaatcag ccagcgtttt
    13621 tgttgggcgg gactggcaac ttgttccatc aaatgaatat tgccctcatc tggcgcatga
    13681 atattcatgg caattgggcc taatcgcgaa tagccagcct cttcaaagac gatagccttt
    13741 gcaacatggc ttaggcctaa accaccatgt tttttagaag catgcggagt aagaagacct
    13801 tcttttttgg caagctcaac tagtgtttga cgtaattcag gggagggtcc gtgtggtgtt
    13861 tcccggggat cgccctccaa agggatgatc ttttgagtaa taaattgcct agtttgatcc
    13921 cttagggcaa ttagctcagg cgatattgaa aagtccatat ctcattgtaa tagcaggtat
    13981 ttaaggaaat ttataaattt tcaagttcta gagccctgta atgattaggg ttttcgctat
    14041 acagggggat catttagatc cactattctt agggttttca ggtttaaagg gcttcgcatt
    14101 gtggtttctg atgatctcat cctacagaca aaaaacttga gcaaatcgtt ttatggattt
    14161 acagcagtta gtaatgtcaa tctcaatgta aagcgaggaa gtattcacgc tttaattgga
    14221 cccaatggag cgggtaagac cacctgcttt aatttgctga ccaaattctt agagcctagc
    14281 tcaggtcaga ttgatttcaa tggccacgat attacgaaag aaaagccagc gcaaatcgcc
    14341 aagcgcggca ttattcgttc ttttcagatc tccgccgtat tcccgcatat gacagctcta
    14401 gaaaacgttc gtattggtct tcaacaagag cttggtactt ctttcttctt ttggaaaagc
    14461 gagagctctc tcgacatctt gaatgatcgc gctatggatg cactccgcca ggttggtctt
    14521 gatagctttg ctgatgaatt agcagttaat ctgccttatg ggagaaagcg tgcccttgag
    14581 attgccgcaa cggttgccat gaacccagag ctaatgctac tagatgaacc cacgcaggga
    14641 atgggacatg aagacgtgag tcttgtcacc gagttgatta aacgagtatc gcagggtaga
    14701 acggtattga tggttgagca taatatgaag gtcgttgcaa acatcgccaa tacgatttct
    14761 gtattacagc gcggcgaggt gattgctgaa ggaccgtacg agcaggtttc aaaaaatcca
    14821 caggtaatgg aagcctatat gggtacggct gattctgagc tacaaggaca ttaattccag
    14881 atgagctcac tggctttaga gatccaaaac ctgaacgctt ggtatggcga gtcccatgtg
    14941 ttacatggca tcaacttatc cgtgcataaa ggcgaggtgg tctgtttact cggtcgaaat
    15001 ggcgcaggcc gcaccacgac tcttcgatcc atcctgggcc tcgttggtaa tcgcactggg
    15061 tcgatcaaag taaatggaca tgaagtcatt ggtatgccaa cctatcaggt ggctcaatta
    15121 ggcattggct attgtcctga ggaacgcggt atttttacaa gcctcacctg tgagcagaat
    15181 ttaatgttgc cacccaaagt gggtgagggg atggcaatgt ccgtggatga gatttatcaa
    15241 atgtttccca atctttatga gcggcgctct agtccgggtg gcaagctatc gggaggcgag
    15301 cagcaaatgc tagcgattgc acgaatcttg cgcactggcg ctagcctact tttattagat
    15361 gaaattactg aaggattggc accagtcatt attcagaagt taacggaagt ggttcgtgca
    15421 ttaaaagaac gtggattcac cattattttg gtggaacaaa attttcgatt tgcttcaaag
    15481 ctcgcagata gaaactatgt aatcgagcat gggcaagttg taaaaacggt cgagaggggt
    15541 gaactcgagg ctagtcagga agagctcaag attttattgg gtgtgtagtt ttaataagtt
    15601 gaaaaaagtt tataaggaga agttcaaatg aaaaaaacag caattgcaac attagtcgct
    15661 tcctgcttta tggcagcagc gattcctgct caggctcaaa cggtttcaga taatgaggta
    15721 cggattggtg tgttaacaga cctctccgga atttattctg cgattgaagg tcctggcgca
    15781 ctcttggcgg ctcagatggc tgcaaaggat tttggtggcc aagtattagg caagaagatt
    15841 gtgattacat cggcagatac ccaatctaaa gcagaccttg cgagctcgaa agcacgcgag
    15901 atgtttgaaa aagataaagt ggacatgatt gtcggtaacg tttctactgc ctcagcacta
    15961 gccgctatgg aagtggctga gcaattcaag cgagtttcga ttgttaatgg tgcagcatct
    16021 ttgcaaatta ctaatgagaa gtgcacccca tacagcattc actatgtcta cgatacctat
    16081 gctctctcac gtggaaccgg taaagcagtg gtcgattctg gtaagaagac ttggttcttt
    16141 gtcacggcag attatgcatt cggtcatacc ctagagcgtg ataccactga agtagttttg
    16201 gctagtggtg gaaaagtatt aggcggtgta aagcatccaa ttaacaccac cgatttctcc
    16261 tcgtttatgg ctcaggctca atcgtctaaa gcagaggtta ttggcttagc taatgcgggc
    16321 ggcgacacca ttggatcggt caagcaagct gctgagcttg gcattatggg atcgaaacaa
    16381 acggttgtac cattgctaat gtttacaagc gatattcaag cccttggctt aagacaaaca
    16441 gctggtatga catttactga aggttggtat tgggacttta atgacgccaa tcgtaagttt
    16501 gctgagcgct tcatgaagga gttcaagggt aaggctccaa catcggttca agctggtgtt
    16561 tattctgcaa cgatgcagta tttgaaagcc gtcgaagcaa caaaaacgga taactcagat
    16621 gcggtcatga aacagatgaa atccattgaa attaatgatg gcctcttcaa aggcagaatt
    16681 cgtgctgacg gtaagtttga gcatgatatg tatttgctcg aagtcaaaaa gcctaatgag
    16741 tctaaagggc ccaatgacgt agcgaaggtt gttaaggtta ttgctgctaa agatgcgact
    16801 ttgcccttag cgcaatcgaa gtgcaaatac gtcactaagt aattgaggac tgctccaagt
    16861 catgtttgag ttactcggaa tcaccccgca agccttagtc tcacagctct taattggact
    16921 gattaacggt tctttctatg cgctgttgag cttgggcttg gcggtgattt ttggtttatt
    16981 gaatgtgatt aacttcactc acggcgctca gtatatgttg ggcgccatgg tggcgtggat
    17041 tggattaact caagtgggta attggattgg gatgccagag tttcagatca actattttgt
    17101 tgctctgttt ctggcgcctc tgatcgtagg actttttagc atcgtgattg aaaaaacgat
    17161 gctaaagcgt ctttatcacc ttgatcacct gtatggcctg ctcttaacct ttgggcttgc
    17221 cttagtgata gaagggatgt tccgtcattg gtttggcgca ttaggtaata gctatcctgt
    17281 gccagagtta ttacagggtg gcattcgggt tgccgaactc tttttgccgt attaccgatt
    17341 gtgggttgtt ttcttagcat tgatcgtttg ctttgccacc tggtatttta ttgagcgcac
    17401 tagcttgggc agtttattgc gagctggtac tgagaacccg cgtttattgc aggctctggg
    17461 cgtgaacgtt ccattgattg tgaccctcac gtatggggct ggttgtgcgc ttgctgcttt
    17521 tgctggggtc atggccgctc ctgtctatca agtaaccgca gtgatgggtt ctaacttaat
    17581 cattgtggta tttgccgtgg ttgtcatcgg tggcatggga tccattgcgg gtgcgattgt
    17641 cacaggattg ggattaggag tcatagaggg actcactaaa gtgttttacc ctcaagcctc
    17701 ttcggtctca atctttgtaa tcatgattat tgttctttta attaagcccg ctggactgtt
    17761 tgggaaatct aactaatgat gaaatccaaa ctctcgacgc tcgataaaat ttctttagtg
    17821 atcattgcac tgttggcatt gattccatgg cagggttttg tatatgacct gtttatgatg
    17881 aaggtattct gttttgcaat ctttgcggca tctctaaatc ttcttctcgg gtttgccggc
    17941 ctcctatctt ttggtcatgc tgcctttttt ggaacgggtg cgtatatcac tgcctatgtc
    18001 atgaaagagt gggggatgac ccctgagctt ggtttgatct gtggagtgct gtgctctacc
    18061 gcattaggct atgtttttgg tgctttagca attcggcgtc aaggtattta ctttgcgatg
    18121 attaccttag ctctcgcaca agttatttat ttcttggcaa ctcaagtgcc attcaccaat
    18181 ggtgaagatg gtattcaggg tgtcccgcgt ggtaagttct taggcttgat tgagctgtcc
    18241 agttcaaccc atatgtatta cttcgcattg atcgtcttta tcttggcatt tttcatgatc
    18301 aagcggattg tgagttcacc ttttggccaa gtaatgaaat ccattcgaga gaacgagcca
    18361 cgcgctattt ccttaggcta tgacgttaac cgttacaaat tgattgcatt cactctgtcg
    18421 gccggtctcg ctggtttggg cgggggactc aaaagcttag ttttacagct tgcatcgcta
    18481 tcggatgttt tctggcacac ctctggtgag gcggtactga tgaccattct cggtggtatt
    18541 ggcaccttat ggggccctgt cgctggagcc gctgtgatca taaatctaca aaattattta
    18601 gctaacttag gcggttggag cacgattgca accggagcca tctttgtgat ttgcgttcta
    18661 gcatttaggc gaggcatcgt tggtgagatt gcctatcgtt taaa
//
LOCUS       DDPQ01000147            4192 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Polynucleobacter sp. UBA2464 UBA2464_contig_11055,
            whole genome shotgun sequence.
ACCESSION   DDPQ01000147 DDPQ01000000
VERSION     DDPQ01000147.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453345
            Sequence Read Archive: SRR5323355
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polynucleobacter sp. UBA2464 (freshwater metagenome)
  ORGANISM  Polynucleobacter sp. UBA2464
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Polynucleobacter.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 18.07x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Polynucleobacter sp. UBA2464"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2464_contig_11055"
                     /isolate="UBA2464"
                     /isolation_source="Lake Lanier"
                     /db_xref="taxon:1947076"
                     /environmental_sample
                     /country="USA: Georgia"
                     /metagenome_source="freshwater metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX331703 metagenome;
                     metagenomic"
     assembly_gap    3166..3271
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1217 a          931 c          849 g         1089 t
ORIGIN      
        1 gtaactcgat gggtgattgg atccaattgc atagggccaa ttcttaaaac ctcatccccc
       61 aagcccaatg aagagttacg tcttaataat gctttaaccc ttgaaataag ctcctttggc
      121 gagaatggct tggtgacata gtcatctgct ccagaatcta gcccctgaat tttatctgct
      181 tcagcaccct tggcggtgag catcaaaatc ggcaaatgct gtaactctgc gctggctctt
      241 aactctttgg tgaactgcac acccgacttt ccaggcatca tccaatccaa aatcaagaga
      301 tcgggtttat ttaccctaat catgcgcatt gcctcatctg cctgtagagc gcgttctaca
      361 gtgaacccag agtgggtcag gttaacagca atcagttctg caatcgatgg ttcatcttcg
      421 acaaccagaa tgcgatgcgg catattctta ttttgtaact tctcgaacta aatcatcgtg
      481 ggatatatga cgaacatccg cacccttaac aatataaata acgaactcag caatattctt
      541 agcatgatct ccaacgcgct caatcgcttt agcgataaat agtagatcaa ggcccgttga
      601 aatcatctta ggatcttcgg ccatataggt aattaactta cgtacaaaac ccctaaactc
      661 ttcatcaatt tgcttatcat ccgacacaac tttggcagct gctactgcat caaggcgagc
      721 gaagcaatcc aaggactggc gaagtagatc gattgccatt tgtccagata acttgatttc
      781 tgctgcgtta attttgtaat ttggggcttg ctcgatgatt cgcttagaac gcttagcgac
      841 ccgctcagcc tcgtcacccg cgcgctctaa attagtaatc gcttttgaaa tcgccatcac
      901 taagcgcaaa tcgcgcgcag taggttggcg acgagcaatg atttcggcac aagcggagtc
      961 aatttctacc tctaatgcgt tcactgattt ttcttgttca atgacttgct cacatagatc
     1021 aatgttggcc tctgagaatc cacgcatggc aatttcaatt tgcgactcca ccattccacc
     1081 catttgtagc aatttgctac agagagaatt caggtcttca tcaaattgtg acgataagtg
     1141 acgatcgttc atgctgtctc ctgttaacca aagcgaccgg taatgtagtc ttcggtctct
     1201 ttcttggctg gcttaataaa tatttgatcg gttttaccat attcgattaa ctcaccgagg
     1261 tacatatagg cagtgaaatc agaaacacgg gcggcttgtt gcatattatg ggtcacaatt
     1321 gcaatggtgt aatctttctt gagctcattg atcaattctt caatcttggc ggttgaaata
     1381 ggatccaacg cagaagtagg ctcatcaagg agaagaaccg atggtttaac agcaacccca
     1441 cgggcgatac acaaacgctg ttgctgaccg ccggagagcg aaagaccact ctgatttagc
     1501 ttatcttttg attcgttcca caaagctgcc ttatttaagg cccactcgac ccgctcatcc
     1561 atttctgctt tagaaagtcg ctcatagagt ctgacaccaa aggcaatatt ctcataaatc
     1621 gacattggga atggggttgg cttttggaac accataccaa tacgagagcg aagcaaattc
     1681 acatcattgc caggctccaa aatgttctca ttgtaaaagt taatctcacc ctcagcacgc
     1741 tggcctgggt agaggtcata catgcgattg agggtacgca ataaggtcga tttaccgcaa
     1801 cccgaggggc caataaaggc agtaacctta cgctcaagaa tatttagatt gatattcttt
     1861 aaaccctgaa agctaccata gaagaaattg agatttttaa tttcaatcgc attcacagtt
     1921 ggggtgtttg aactaacatt ggcaccctgc gctgactggg cattaccttg attaatatcg
     1981 gaatgaaaca tcggtttcat ggtggttgtc atacgcttac cttttcacga agtgctacgc
     2041 gggctatgat attgatgccc aatactgcaa atgtaattaa aagagctccg ccccaagcga
     2101 ggttgaccca attgtcatag ggactcatcg caaactggaa aatcattact ggcaaattag
     2161 ccatgggcgc gttcatatta gtggagaaga actggttgtt gagcgcggtg aatagcaatg
     2221 gtgccgtctc tccgctcaca cgggcaattg caagaagaac gccagtaatg acaccacttt
     2281 ttgctgcacg taagacgacc gttaaagaaa ccttccactt aggagcaccc agagcaaatg
     2341 cagcttcacg taatgtgctg ggtaccaatt tcaacatgtt ttccgttgtt cgaacaacaa
     2401 ctggaatggc aattaaagaa agtgctaccg ttccggccca tcccgaaaaa tgtttgacct
     2461 gataaacgat gatggcataa acaaataagc caatcacaat ggatggtgcc gacaacataa
     2521 tatcggttac aaaacgagta acttgggaga acttattatt gtctccatat tctgataaat
     2581 aaatacctgc caatatgccg attggtgtac taaggaaagc gcaaaaaaat accatcaata
     2641 agctaccaat gatcgcattg gctagtcctc cacctggggt tccaggcgcg ggggaaattt
     2701 ctaaaaataa tgctgggcta attgcggaga atcctttaaa caaaaggatt gccaaaatcc
     2761 acaacaaaaa tatcattcca attgacatgg caactagaga gaatgtcaaa ccaaacaaat
     2821 tggctcgttt gcgcttagca aataaagttg ggtcacggga gatcattatg tttttgctcc
     2881 ttgatttgct tccattcgca tcagcatgag cttagccagc gttaatacaa taaaagtaat
     2941 tacaaataag gctaatccaa gggcaaatag agaggagtaa tgcgcaccca cctcagcttc
     3001 gccaaactca tttgctaagg tagatgcgat cgaattacct ggggcaaaca atgacgctga
     3061 taacttgtgc gcattaccaa tcacaaaggt aactgccatg gtctcaccca gggcccgacc
     3121 caatcccagc ataatgccgc caataacacc cgtcttggtg taaggnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtgcccgc taaggctggt tgtatgtact
     3301 gcgcaaatat cggagcaaag ataaatagac caaacattcc ataaatgatg gatggcaccg
     3361 cagccaaaat ctcaatggct gttccaagtg gtctgcgcaa aattgatggg cacatttcag
     3421 ttaagaagac tgcgattcca aaactaagag gcacagcaat tattaaagca atcactgagg
     3481 tgatgatagt gccgtagatc gcaattaagc caccaaactc gccgttaaca acatcccact
     3541 caacagttgt aaagaaacca aatccaaatt tacttaaagc aggtgcggca ttaaagataa
     3601 gcgatgcaat gattcccaat aatgtaagca aaacagagag ggcaaaaaac tgaatagtgt
     3661 atttaaatac tccatccact acccgttgtt gttttgcaat gcttaaaacc ttggggctaa
     3721 ctgtattgtt attgagtacc gcatccatgt gtgttcaaac ccttttagac aaaatcagcc
     3781 ctagagtatc tagagctgat tttaagacaa actttggtaa ggattactta gtattaattt
     3841 gattccaaac actcttacga atgaagtcgg ttgttgaatc tggcattggc acataatcaa
     3901 gctcagcagc cattttctta ccctctttga aagcgaagtc aaagaacttt aatacttcag
     3961 cagagcgagc tttgttacca ggttctttgt acatcaggat gaatgatgcg ccagtaatag
     4021 gccatgactt cgcaccagca gcattggtaa taaatgtacc catgcctgga atctttgccc
     4081 aatcagtacc tgccgctgcg gcagcaaaag tgtcgtcatc gggttgtaca aattgaccag
     4141 ctgggtttct taattgaaca tgggtcatgt tgtttttctt ggcgtatgca ta
//
