LOCUS       DDWP01000001           12946 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_16301, whole genome shotgun sequence.
ACCESSION   DDWP01000001 DDWP01000000
VERSION     DDWP01000001.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12946)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12946
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_16301"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3485 a         2940 c         2304 g         4217 t
ORIGIN      
        1 cctgtccatt cccaggacga cattaaattg ctatctatcg acaaaaatcc caatatcacc
       61 cccgaccacc tccaggaagt catctctcag tcggccggtt tggctcgtgt ggtcaagtat
      121 atttctctca aagattcttt ttctccgggc tggtgggtta atgacactca gcttcaacgt
      181 cttatccagc ctactcttgc ggcaattagt cggtcaagcg atgaagatct caatagtcta
      241 ggtctcagac aagacggtca atttcaaagt gaattaatct cggatcttgt tagtagaatt
      301 tcttctacca gttttgatat taatataagt tcagatctta ctattatcga aaacggtcaa
      361 aactttggca gaaaatctag tccagttgaa aaggcattac tcgtagaaat gcagtccaat
      421 aatggtctta tttccaaaga aaagattgcc gagatcaaat ggggtcaaga cagttatgac
      481 agtttttctg accaagcgat taagaaaacc atgatgcgtc ttagtgttaa gcttcgtaaa
      541 taccgtataa aatcgattac caagtttggc tatcagctct cccttcgatg accttagatc
      601 taagagatac cggcttacac actgaagtca tcaagccaac tagtcattct ctagtagata
      661 ctgctcgtac ctataaattt tacgatgtcc actcttatag tagtccagaa ctggccaagc
      721 aatatggttt agacatcacc gaatttattt ctgttgttga tggaaataga cgccttattt
      781 tacctcttca ttacaacact gaggatgata atgctcaaac cccaaatcct tgtttcattg
      841 tcgacggtca accacagtca gttgccggtc ttcaccaaaa tattgtcgcc cacgagaact
      901 ctcgaccggt tgaggagttt cagacccttg aagttgatga taactttctc aatctgtttg
      961 aatatggtat tagcgttctt aatcttccgc ccatagtttc tgcctatttg gctcaacaag
     1021 aaggtatgac taccgataga ttattgaacg aatatcatcc caacagtccc caagatttca
     1081 cctccgttat cgctgtttcc ggtccctctc gagccggcaa atcccagttt ctcgctattg
     1141 aagtctttaa aggcaatatc gctgttgtcg cttccgagcc attttctgat tatggtttca
     1201 agaattatct gcaggtttta caggacttta ataatggtcc aaatggtcca ataacttatt
     1261 taggtgacga ggcactttta gatatcatta atagtggtat gaagcagatg gaagattctg
     1321 gcaaatttcc tccccgagtt attcatatca gtgacatgct tgaggagatg agttcttact
     1381 ttaaaactgg cagaattcgc ccatcaacta tttactttga tcttaatggt tatactccag
     1441 atcatccccg agaaccccat caatacgatg cccttacctt cttcgcctgc gacactttat
     1501 cacttcttgg tcgtggtttt gaggctgttg tagatcacca ctggcaagaa gtttttactg
     1561 acaaaagagc tctcgatgtt aatgctgaag ttattgaaga ttatcgccga caacttactg
     1621 atggtcagga tgatctttat aaattagcct atgcttcaca aggtcgtttc cgccaacgcc
     1681 tcgaaaaacc tcacgaccgc tttttgtttt agtctcacta accgcgaata accgacaatc
     1741 ataaacttag aatttgttgt aaaataccct cacgaatatc tacttttctg tcatcaaatt
     1801 taattttcta aaatttctga cctatccttc agagatctta gccaccgttt tccgtcggac
     1861 tatggaggtt atctttttgg ttgttttttg gcagattgct ctagacaaac caatttccga
     1921 gataaaaact ttggtctctt attttctagt agcccagtcc gtttccttaa ttgttatggc
     1981 ccgtcgtacc gagtttggca cagagctcag aaaagctatc aaatatggca ctatcaatat
     2041 ttatctagtt agacccatta gaattattcc ctacctttac gccaatacag ttggcaatca
     2101 aggtttaagt ttagttcttt caattatcct tattgtcgtt agtatgttta tttctccacc
     2161 atctaacgcg ttgaatattg tcgcttttct gttctccact cttattgctg ttggcattgc
     2221 tctgtctttt aatttagttg aaggtatctt tgcttttcgc attaccgaga ctggtcctgt
     2281 cgctggttca atcagaagaa tcattgaagt tttttctggc gccattgctc cactttcttt
     2341 ctttcccgac tccgtcacca acattttaaa attcacccca tttcctgcca tggtctatgg
     2401 ccccgtcacc agtttagaat ttggctttga ttttttacag ttaggtgttg gtttattttg
     2461 gatgataatt cttaatctct taacttttaa attgtggcat atggccatca aacactatga
     2521 agctattggt atgtaagaat tttatttccg atagagtaga aattctcaag gccagtattg
     2581 tttataactg gcagatagac tcagcctatc catttgccaa cattggcaga atggttggta
     2641 gtgtcttgta taccattacc acccttattt acatcaatgt tttgtatcaa aagctccacc
     2701 ttatcgctgg ttacagtcag caagaaatgt tggtcttttt cgccattggt caagtcgctt
     2761 tatttaccat tgctagtttt agttacgaca acattatcga cttaattgcc tcggttaaca
     2821 ctggtggtct tgatctaatt ttgacaaaac ctcttccttc tttgttttat gtttccatca
     2881 aaaatattgg cctctttcga attcttgtcg agaatattgg ttctcttact atcatgttct
     2941 tcttcatcaa atggtctaca ttgtcgatca ctttttatag ttttatcagt gcgctcattg
     3001 tttttgtctg tgggcttatc tccatgcacg tcattcaatt tattgccgcc ttaccagtat
     3061 tttggatcgg tgagagctct agtcttctcg attctacttg gtcctttgaa tacaatattg
     3121 gccgaaccat accctacgaa ggtttctcag ctccattcca aattattttc accaccctct
     3181 taccatctct aattaccact ggtgtcaccg cttctgtctt actctcaaaa tcaaatccac
     3241 ttctaatggc tatttattgt ctcgcggttg cttctttttg tttaattatt aaaaatttat
     3301 tttggcggtt agctctcaaa aactacacct ccgcctcatc ataaagctat gaatgtcatc
     3361 gatattgcta atgtcagtaa aatttttaaa gtcaaaataa aacacggttt ctggagggat
     3421 atgttcaagc cagaatatcg tcatgtctca gccgtcgacc atatttcttt ttcagttaaa
     3481 aaaggggagt cagttgcttt tcttggtcca aacggtgccg gcaagacaac tactatcaaa
     3541 atgcttactg gtctaattta tcccaccacc ggtagtctgt ctgttcttgg tcacaaccca
     3601 aacgaacgtc agcccgacta tcttcgcaaa attggtttag tcatgggcaa taaagctggt
     3661 ttaaattggg atcttacctc ccgacaaagt tttgatctat ttaaacaaat ttaccgtgtc
     3721 cccgaggaaa cttttaaaac aaaattaaac gagctcaccc agcttttaga ggttaaccaa
     3781 tacttagatg ttcaagttcg taaattatct cttggtgaaa gaatgaagat ggagttgatt
     3841 ggctcacttt tacatagtcc agaaattctt tttcttgatg aaccaaccat tggtcttgat
     3901 attatttcca aacaaaaaat tcgtgatttc ctccgccata ttcagaaaga atcaaacatt
     3961 actatcctcc ttactagtca cgatatggat gatgtcgaga aagtttgtga cagagttatc
     4021 gtcattaata aaggcaaaaa agcctatgac gacaagttaa ctgtactaat gcaaaaatac
     4081 aatcagcacc gttttgttaa attcattttt gccaactctg tcgacttagt ctcgttgcct
     4141 tttggggagt tagttgaagc agataatcac agtgctttgt ttaaagtcgc cccaaaactc
     4201 ttgccagatt taattgccaa agcagcagaa aaatatcagc ttgatgacat tgacattatc
     4261 cccgtacctc ttgagtcaat tatcgccgat attttcaaaa ccaaaaaata atcctagcgt
     4321 ttctctgttt tttgtggggt tatttaattc taacaaacac cttttcggaa tgacactcat
     4381 tgattgcttg tagtagccac tgtataacaa caagactgct aaatacttta aatgattaaa
     4441 catcaagaaa agtatcctgg tcggatgatt gaaatatccg aaaagattgc ccaagaaaag
     4501 acagtaatca tgcccgacaa cggccccatc cgtttgttgt ttatctccga cttacacatg
     4561 ttcgaccctg atagtagtcg ggagtatatc aatcaggttc tcgaagaact aagtaaaccc
     4621 aatacttatg ccgttttgct gggagacatt attcaaggct tcaaccctac ccgtgccagc
     4681 atggctgctg gtacccctcg acttgacgaa caaatgttaa ctgctgaggc cattttaaaa
     4741 ccatttgccg accagggaaa aatcattgct gccgtcggtg gtacagacag tcacgaaggt
     4801 tgggcagaca aaaatatgac ttttgatgcc acctacttta tgtttcgtaa tctcaaaggt
     4861 gtcgacggtc aagacctgcc aataatgaac aatggtggtt atgttacctt acaattccca
     4921 aacggtggta agcaccgtat tcgtgtttat catgatgctg gtggtggtgg ttcagccaaa
     4981 aacatggttg ggtcagaacg atctagggct atggaaataa ctgttgacga tcctctttgc
     5041 cccgatcagg ttgttggtgg ccacactcac agtcgagcca tgatcactac tgagctagca
     5101 attaatgctg ccaccggaga ggttatctct cagacttttg ctactggtgg tacagctcaa
     5161 ggaattgatc ccaatcgccc caatgtattt ttaacagcca gaggtgtcgc cccatccctc
     5221 cctggtcttc cggccactat cctgactcct catcccggcg atgggactac ccgcagagac
     5281 gtttggggtc tcgagaactc tggtaaatta cttgaggccg tcagattatg ggaggccatc
     5341 gagtctagaa acttggggac cgaaattgag tccatgatcc gtgaaaatat acccactact
     5401 caggcagaat ttttaccaga ctcctctcgc ccagttgttc gtaccagtgc cgatcaagat
     5461 atacaaaacc aagtctacga tgtcgccagg tggcacgttc gatctgccgt tcctttactt
     5521 actgtttttc taggaggtac ccgttatggt tcttcctcaa ccgacgaatc tgccgccaga
     5581 aaagttgttg ccgaggctgc caataaccct gaagcattct taatcattac tcgacgtatg
     5641 gttgataagg gtttgtcatc cgatccagat agagatgctc atttgtcaga tatggttgat
     5701 ctaattgagc cagccggtcg taatggcaag attctggcat tattaatgga cgacaaaggt
     5761 cttcgtgatc ccggttggaa gaagccaatt ggcgattatg aatctggtga cggcagtagt
     5821 ccggttgtta ctggcgatta tattcaatct cgtctcccgg tacctttaat ttacaacggc
     5881 tcatttgttg ttcttgatgt aggtggtgtt gcctatcgct attacattag agataagttg
     5941 ggtaataatg gctcgagcat caaccccttt gctggcctag aagttcaggt tgctcgtact
     6001 cgaactggtg tcgacgttgc cgctggtggc cacatgcctc gagttggtgt ctcgcaaact
     6061 gcttcccgtg tcgttgtcgc ccccggtggc tatgctcgct ggtctgaaat gagtaaagga
     6121 aatgaagaac cattgccaga aggtggccaa gcagtgatta tttatccagg ggaaaagagg
     6181 atgattgctt gtgctacttt tgtggaagct cgggatactc atcgggccgt tttctatgcc
     6241 acaggtcttc aatttcttcc tcaaaaaact caaaccaaac tccttaaaat ggcacgacaa
     6301 aatatatctt gatataatca cttatgccac gatccaagga tctcgatact atcgctccag
     6361 acgacttaat agtcgtagac tcagtacctg agccaaaaaa acctactcca gtcaaaaaaa
     6421 ctgttgttgt caaaaaaaca gtacccgtca ttgaggaacc tgttgttgcc tccgacaaca
     6481 ttctggctca aaaaatatat gatatttttg gtcacactaa gtctcccttt tctgctgctc
     6541 tagtttttcc taaagttttc agctttgccg aacaaaacga cggcgaagtt gttcttctgg
     6601 ctcttcgatc tcattggttt accaacacgt cttggattct aatggctata tttatgttta
     6661 ctgttccctc tttagttcct ttgatcccta ttcctggatt agatggtggt ctccgtatcc
     6721 tcctctcttt tacatggtat ctaattactt ttgcctattc ttttgagaaa tttatatctt
     6781 ggtattttga tgtgtctatt gttactaatc gtcgcgtggt cgacattgat ttcaataatc
     6841 ttcttgataa aaaattctcc gaagctgagt tgtccaagat ccaagatatc agttccaggg
     6901 tttctggtgt tagccaaact tttctaaatt acggtactgt ccttatccaa actgccgccg
     6961 aacaaaacgt tatagttttt gagaaagttc cttttcctgg taagatcacc aagttattac
     7021 aagatttacg attttctgaa tctgaaaaag ataaagatgg aggccaagaa taatgattga
     7081 gaatcttagt ttttatttct acctttttaa gaaaatctct ctccttttag gtgctggtgt
     7141 ctaccttgtt tttgccctta tcgttgtcaa acagacattt accatgagca aaaacatcaa
     7201 cgacaaattt aactatatcc tcatagcttt ctcatacata catctagctt tttcttttct
     7261 tctcatccta ctgacgctta tcttagtata gtcaaaagct tgactatccc ataacattgg
     7321 gagtataatc tgttttgtga atcaattacg taaagattgg gaaggtcttg ttcaaggtgc
     7381 ttgcgctaac gcggctacta caggtcttcc tagccgtgga atgatataca tgtccccaca
     7441 acttcgtgac agtcgtggta atttaattga gcctaaaact aaaggtccaa aatggctcca
     7501 aatcgctgcg tctggtaaat ctcctgaact aaacaaagaa tttgactggt cctaaattac
     7561 tttagccact ttctcccaat aatccgcccc ttcggcatgg ccaccagttt taaaaatctt
     7621 cacttcaaag ccttcttttt tgagttttgg tccacataat agtgccggaa aggaattatc
     7681 atcttcaaat aaaatggcca tttttttagc ttttattttt tccattttta tttctctcat
     7741 aacttttatt agacttccaa acactgcata tccctgttct gctttaaagt ttcttcccgg
     7801 ttttggtaaa ggaaaatctt ttaaattact ttgtaaaaac cattttgtta tcacgaaggc
     7861 caagattcta gatttaaaaa acaagctcgc catgtttagt aatatcttta atcttgtatc
     7921 tagaagtggc actgttgatg agctcaccaa aatcaatttc tccgtttttt caggatattt
     7981 attagcgtat ttactagcga cgaatccacc catagaaaac cccaggatta tcgctttatc
     8041 tttaactttt ttatctacaa aatctactaa ctcatccaaa ttatattgct tttttgggtt
     8101 agaaaaataa gggtagtcga ttgtctctac cgagtatttt ctactaagta gtgggacaag
     8161 ttttttaaat gttttctctc cacttccatc gtaaccgggg agtagtaata tctgtttttt
     8221 catctattta gctgtctctg tttctttaaa cttttgtagt gactctcttg tcaccttacc
     8281 atcaaccaca tcttgcaagg taaacactct actctcgaac tggcttttat ctaatttccc
     8341 tctgtctact cccagatgtg ctcttgttgc atctattaga tcctccttaa tatcttcatc
     8401 cggcactatg aataccattt cagttggtgg tataaactta gaaattctct ctccgttttg
     8461 atattgcaca tatttcgttt cttctctccc acttattaat accgtcgcaa accccctaaa
     8521 atggtcacct gccaatgtat taccttgttg tccgaaatta ccgatataag gctctcttcc
     8581 cccagaattc tggcttggcc acaaaccaac tatcgacacc atctgtaagt tgtacgctaa
     8641 tcgtcccaca tcagtcatca ccccaccgtg atttgtcggg ctatcagtta tcgaatgtcc
     8701 tatcaatctc tcttcttcca gtttagctct cgctgcttcc actagaggtg gtaaatcttc
     8761 tctctctagt aggcctctag actctgtcac taggagtttt aaatgacgtg ttgacggact
     8821 ggatagtttg ggcatttcta gatcatcatc ggccatcctt ctcaaaaaaa cgtcctgagg
     8881 catccccgta acctgggata caccaaacca ttgctcttca gctagcattg tatgttctct
     8941 tcccggcagt ggcgtcatat ctaactccac catcatcgtg tctattgttt cattaccagt
     9001 tgttgtcggt gttgccactg caatttgttt gctgtttgtc accaaaccat ccaatcctgc
     9061 atctttaccc atatttaaat ttcaatttga ttatctttat aatatccggc tggcatagtg
     9121 taggttgccg tgtcttctct acagccagtt atctgtctaa acaattctct catatcctcg
     9181 cttgattgtt cttctggttg gtgagttcca ctccttatac cttctctctc tttttgcata
     9241 actccagcac agatacgtta ccaatgcctc tgtttcttca gctgtggctg tttcagttcc
     9301 accatctttc cttttttcca gcaacgacaa cattttaggc accataccca acagctctcg
     9361 tagttccagg tcgatcagac caccattgtc ctcatgagta ttcaacatac ccatcacgtc
     9421 agatataaat tgttgtccca agtttcttac taacactatt acctcccctt tttcatctaa
     9481 ccgtattaac ggtggtcgat gttctaccaa tctcatattt caaaaaaatt ttaatcatgc
     9541 tttcgctctt ccgagctcat cagtccagat ggtgacgaag tcataatatc tggagagcat
     9601 ttatcaccag tcacattcgt caaaagggga ggcaaagcct ccccaatcgg cggagtgtgc
     9661 tggtgtcagc gtgttacttt actgatgggt atatccacca gatctcttct tttttgttat
     9721 cccagtaatt tattgagaac atcggacgat ccttaccgtt cgggtggtcc cagttatcat
     9781 tacttattgc aaggtttcca ctctgtgtaa tgaatctaaa accccacctt ctgtctggtt
     9841 caccacgccg atcgattgga gcaaaaaaag agatgatcga aaaatattta tcttgtgata
     9901 tttccatgtt tgttaccgtc gacatccata gtgtgggttc gatgccaact tcggtccaat
     9961 catggttgta atctgtatca cgtccaacac acaagatact tctcgggtaa aacggtgtcg
    10021 caaaatccca gcaatgggtt acatatttgc ttgccacgaa cacagcaatt acaacagtca
    10081 atagaacaaa cagttgggtc ttacggactt tgatactttc caagatactt ttcatttcct
    10141 tctccttttg gggttttaaa ctaacacgct gaattttaaa gtgctgagta ttcgccaacg
    10201 cggtgttggt tattctctgc cccgaaaggg gactggctta agttgaacag ttccaactaa
    10261 gttgagatta ttatcttata ataaagaaat ataatctgga caaaacctga gacaagttga
    10321 gacaagttgg gacatgctat gataggtata tatgttggaa gatcggctag cttttcagag
    10381 actatctacc tggcttgtag atgctaaatc aaaaaatatc aacgttacta ttgtctcctt
    10441 accaggcatt ggtctatctc attaccttaa agtctattcc caagaaaaca accaagacgt
    10501 atcctatatt acagatatca attcgcttcc aagtttaaaa tcacaaaata ttatcgacat
    10561 cagttttcat caatctccag atatttatga atcaatcgat aaagttgtcc tcgcctctga
    10621 tgttaatcaa aaattcataa ttgtcattga cgatcccagc tggctcagaa gtgcccacaa
    10681 agacaattgt cgtttcctct ctcgtgttta tcgtactgaa tttttcccca ctcatgcgcc
    10741 ggaagatatt aaggccatgt ctctagagat aaatccaaaa ttgtccccag ctcaactgaa
    10801 agagatcgtt gatcaatctt gtggtctggc ccgacttatt aaatacttat ctttaaattc
    10861 acccttggat gataatttaa aaattattac tcagccactt atttccgttt tatcaaaatg
    10921 taccgatgaa gagataacta aatttggtat tcaactcagt ccgctcatta ctaaattatt
    10981 aaaagaacaa tattctattt ctggatacaa aattagcatt aacaacgatt actctgttac
    11041 tgagggcgac tgtaaattca gtaacatatt gagtctcccc gagagagata tactccaatt
    11101 tatcattgac aatcaaggca aaattagcaa agataagatt gccgaaatta agtggggatc
    11161 cgacagttat gatagttatt ccgatcaagc tattagaaag accgttgttc gtcttagttc
    11221 taagttaaaa aaatatcgta ttcgaccaat ttccaaagtt gagtaccggc tcgaaccacg
    11281 atgattaatc cccaagtcga gaccaatgtt cctattaccg ttgccaagcg tgagattgac
    11341 caacctcttg ttccagggga cattactcgt cttagcgtta ttggtaatac tgatctcaac
    11401 cctgactcaa agggaaatcc tcaagttcta gatattattc gagttcaaga tggtagggga
    11461 cagattacct tacctctaac cttcaatctc aaagatcagc ttctcaatca aaagactact
    11521 ggactatccc ccgaatcagt tgttggatta attcagcaac taacactatc gtcatctggt
    11581 gaagttgctt ctaatgtctt gctggaacgt atggctattt gtttagttga gttacgatta
    11641 cccccactac tatcagcata ctttgctggt caagaggact tgtcaatcga ttatcttcgg
    11701 tcacgtttta atccactcga ccagaaaacc aatactaaat tagttggtct taccggggct
    11761 tcacgttctc ttaaaactac ttttctcgct attgaacaat ttcgtggcca ccttgttgcc
    11821 gaaggcattg aaccgttttc tatttggaca cttcagaata tgattcaagt tctaaaaaat
    11881 agtggtctta atccaaaaaa ggctaccgat gcccaattag tccaggctat tcatgacggt
    11941 tttgctaatc tatcaccaga aagtaatctt gatagtacag gagacacctt atccactcac
    12001 ctcgataagc tcgccagtca ttttgagacc ggcaagaaac gccctgatac actgttttta
    12061 gatttaaccg gtatttccac ccaaagagag cgacctcttc aaacaccaga cctaatctct
    12121 ttttttggtg ttgacgctct gctttatctt gaacgtcaca ctcccggaac taatcgtcac
    12181 gattctccta ttgatattcg tgtcttaaga gattatcgtc gacagcttaa acgacttggt
    12241 ctccacgtta aggctattgc tgtcaacgcg cgcgatgaat gggttcgtaa gcttgactgg
    12301 gacatgacag ttcacaagcc ccacaaacaa ttgtccttct aactttgtaa atctccccca
    12361 aaccggctag aatatttata tagatgaaaa ttctcaatag ttatttttcc ccctcttgtc
    12421 aaagaggggt taggggagat tgaaagatgc actattctac tcgctccttt attggccacc
    12481 ccaacgacac ttcttggtcc caatattggg aaaatgagcc agacgattac tccctaaaat
    12541 caacccatgg ccatcttttt ggccttatta gtttacatgg cgaagcctct tcccagagcg
    12601 accttggccg tgaaatcatc caacagcttg attccaacta tttttcttcc gcctttgctt
    12661 ctcctgccca aaatcttacc tctgccctcc aatttatcaa agataattat cttgccaatc
    12721 tttcacatct agatctggtt gttgctgtga ttattggcga cagaatctat ttatcttctc
    12781 ttaattctgg tcaggtaact ttccagcgca cttcacagat ctctctttta ctcaatccca
    12841 ctcctcaaga gattagttca atctctggca ccattgttcc aggtgacagg tttgttctat
    12901 ctacccaagg ctttctcgac caatatggtt gggatcaggt taagtc
//
LOCUS       DDWP01000002            7560 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_22961, whole genome shotgun sequence.
ACCESSION   DDWP01000002 DDWP01000000
VERSION     DDWP01000002.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_22961"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2070 a         1778 c         1333 g         2379 t
ORIGIN      
        1 ataattcttg gtcagactgt tggtctttta gctatgtttg ttggtaactt tatttatggt
       61 tttactaagt accgtaacga caacaaagcc ctcttccctt attccaaagt ggtcttccct
      121 tttggcactg tcttactgat cactcttttc tttaagttcg cctttagttt gtaagatatc
      181 cttttctggt tttaattctt ctggcaatat tcctcgcttt agtagcgtcc ttcttgtggc
      241 tacgctattt tcataaactt cttgatttaa ttttggtttt ccaactaagt ttttttcttt
      301 gatattgtga tccgtcattg ccaaggcaaa atcttttgcc tttaattcca agaaagaatc
      361 aaaatctgcc aaagctctgt ctttaggaat ctttctttta ttttttaaag tccttattcc
      421 gattccacca tataaacctc gtatatgttt gtctttaaat tcagcaaaat ccctcgactg
      481 ttttatacct cttttgtaaa cagttgattc aatttttctc tccgtttctt ttagtttttc
      541 tctggcttca attcgcttta ttgttttttc tttttcctct acaatttcct gttttcttgt
      601 ctgcaccgca aaataagtct gagccttagc tatttctgtc tttcggctat ctccattttg
      661 agcaatcaga tagcaagcat atcgagttag catataatct ttagtttttc gtttactacc
      721 taaacctaaa tcaaccattt tgtcggtgcc gacaaaatga cccataacat catgatcact
      781 ttgggcacat gactgtttag ctttttcaat caccatctca aatttcctcc attctacata
      841 ccccaacacc ttcatcagct ctctcgccga ccagtattca aaatcattgt ttgttttttt
      901 aattacatcc aattcattca tgattcaata tagcccgaat aatatccgaa tgtcaacatc
      961 ttttgcctgc tgtgcccgcc gtagccttgg cgtaggtggg gtatgaccta tggatcggta
     1021 tttacattga cactaataag caccgtctct atttttaccc cctccccacc ctcttattta
     1081 tcattcagct ttagttagtt ttagttatct ctgtcttaaa gaatttttct gccgtctcta
     1141 ttaaaagttc ccagtttcta taactatgac tatcgattcc tacttcatta attactttac
     1201 aatcaacctt cactttttct tcaactattt ttcttacatc ttcgtatcca gctagtgggt
     1261 caaattcatt ctgaataaag atcgtctcta catgtaattc ttctagatat ttatcgattt
     1321 cataaccaga agcttttgct gttaggtagg gtagaccgca gataattaac ttttttggtg
     1381 aaattgtctt ttcgtatata tttttaagtg caattatcga cccgattgat ttagcaaaaa
     1441 caacataatc ttttgtatca ccaattaatt tttggagatt aaccgactct ttaggtatgt
     1501 cagcccattc ttctccggta tcccagtgta tatagtaaag tatttctcca ctggcaaaac
     1561 catcaaacct ttctttgact tcctcgatcc acactttgtt gttgggacta tttccaggta
     1621 gataaattat atacatggat aaattgtact attaaatccc tcttgcctgc aggcaggcag
     1681 gaaagtcaaa ttatttttca gctctaatca tcttaaacaa tttccacgtt aaccatacag
     1741 ctaccacgct aaggccttgt gctgtaatta gtgctagtct ggggccaacg ttatctgcta
     1801 cccagccaag agataccagg cacatcccat aaaatatatt ttttgccagt gagttcaacg
     1861 aacccattgt tgccctttgc ttgtcactaa attctttttg tttgagtgag ttttgcgcta
     1921 ccgtagttgg tccaaaaaga attgaggttg atgataatat cgctggggat aaagcagttg
     1981 gaaatataaa cgcaactagg ttcacaatca tattgtatat ttgctcaaac agtagaatat
     2041 ttatagcctt aaatttatta ataatctttc cactccatcc aaagcctact gctgccccaa
     2101 tatttgataa ggtgctagct attccgattg cccacgttgg ccacagtaat gatatgaaaa
     2161 caggtctaaa agagaaactc gcttccctca atccaaatcc tatgatttct gtcaagctta
     2221 acagtcgaag tttttcattt tttaaaaagt tatttatgga atctttcaaa tgagcatata
     2281 tgttagcccc tcctttggaa cgcgttccgg tatcaaccaa ccaaaaacta cttataaatg
     2341 aaattatgtg cggaatcacc gacaaccaca taactagtcc tacagaaaaa acatacaaga
     2401 agcctccaat tatcgaagct attcctattg accattgttc agctgaacct atatatccca
     2461 ggtgcttttc caagtcttcg cttttatcaa gtttttgtaa actgtcatag agcatggcgt
     2521 cattatttcc gctataaaat gaccttgcca aaccttccag taccgcccct ataaataaaa
     2581 accaatagct tatccctatc gcataaaaaa caaatgataa cgcatagaag aagttaccca
     2641 gcattgtcgt atattttctt cctatcaagt ctgaaaaaat acccgtcgga atttcaaata
     2701 ttgctgcact taccatctct actgccaaga cgctcattcc taatgtgtat gaccccgaca
     2761 ctttagcaaa gtagataacc gccagaggtg accacaagtt aaagccgatc aaaaacccct
     2821 gccaagttag tattttaata ttcctggaat aatccataaa agtattatat ttgctaagtc
     2881 ccccttgtca aagggggatt taggaggatt ggattggtat aataatctat gcaaaaaaat
     2941 attgatctcc aaaaattcat ggataaagtt aaagccgctg aagctggtca cgagatggat
     3001 ctagcctcag gtgaagatct ttctatggct gttatgaacc tcatcgctat cgaagaacat
     3061 atgtatttca atgccaataa aactggcaaa gtcaaatatt tcgaattact ccacgagact
     3121 cgcgagattc gtaaagtact tatgaaaatg cttatcccca aatatgaagg tgaagtctgg
     3181 tgcaccagca aacatcttct tgccgcctct atgcgcgtca tggaagttgg taccaaatat
     3241 cagaccaaag gtgacaccaa aactgcctat gatctcttca aaaaatccta cgacctctac
     3301 aatctcttct gggccataaa tttaaatatg acagatgcta aaaccatctc ttctgattct
     3361 ctcccaaaag aaaaaaatga gccagaagtc aatctaaaaa aatccgactt tctcggcaat
     3421 atcagtggcg ctatcggcaa aattctagac tgttgtcgtg agtaattgct cctgccagcc
     3481 tttaatcact tcaaagcctc ctctccaaaa attctcatcg ctcatatcta ctccaatttc
     3541 ctctaatatt tctttagggt ctctcgaacc gccggcttca agtaagttct caatttgttt
     3601 tacaaatcct ttatcttttt tgtatctggc atacaaagat aacgatagca attctccaaa
     3661 gctataggca taacaataga aaggggattc aaatatatgg ctaacataca tccattcata
     3721 tttaaaaaca ttatcaactt taactcctgg cccaaattgt tctttgagtc ccgatagcca
     3781 taattttgat aattcttttt ctgttgtccc tttagccacc attttatggg cttcaacttc
     3841 aaatatttca aaataagcct gtcgtaatat tgtggcgtac gattcaccaa ttttttccga
     3901 caacatctgt cttttcacct caatgtcttt ctcttttgct agcatttttt caaacattat
     3961 tgtttccgcc aaagtcgagg ctgtttccgc caaaggcagg ttagcatgtt ggctactggc
     4021 gtaatgtttg ttggcataca aactgtggac tccatgacct agttcatgag ctaaagtcga
     4081 cacatctcga gtcgtattag tgtgatttaa catgacgtat ggagttatct ttggaccaac
     4141 tgtcgcacaa aacgccccac tcctcttatt tttcttaatc tcccaatcga tgtgattttc
     4201 ctcaatcatt tttctagctt cattagcaaa cctggggcta aattcttcaa aactctctaa
     4261 caccatcttt ttactctctt caaaaccata ttttctttct ttaatttttg ttgtcggagc
     4321 ataaaggtca aatctactca gcttagattt tttaagcatt ttccccttat attcaaaata
     4381 tttccaaaac acctttcttt cttctctgca cactttcaat aatgtttcta ttgccttatc
     4441 gggtaaatga ttagcaaaat ttcttacgga gatgggggaa tcatagcctc taatttttgc
     4501 ctcaaatccc cagtctctca ctaccgcctg atacatcaca aaaaacttgt cactattctc
     4561 ttggtgtttt ttgaataaag cccgataagc cccctctctt aattttgggt ttttagagtg
     4621 gaccaaagac atcaattctc cttgagtttt aatctttttc tttcctagct catatacaaa
     4681 atcagcttca attaatcctc ttagatcact aattacttgg ccacccgcta cgtccttatt
     4741 atcaattatt tcttcttctc tttgagtcaa agtatgtttc gcggccaatc tggcataatt
     4801 taatccgtat tctaggtctg gtattgcctt aaataatcgg ctcgcactca tatcatccaa
     4861 agtttttttg ccttcgaccg gtaacccttt taaccagtgg cctactttac ggcttttttc
     4921 attaaacttc agtactaagt tgtcgacctt agttttcatc aatctcgcct gagtgtcttt
     4981 ttgatcaact gattctttta acattggtaa atatcctagt cggctaaata tctccccgac
     5041 attctcgtca aattctagaa attttttgaa ctcattcaag ctcatgtctt ttgacaattt
     5101 ctcccaccat ttctccatca attcgataga cttttccgcc tcagtcaata gctcctcaaa
     5161 tttattatca ggacagacat cgtctaggtt ccagccattt ttttgcatga tttattgtat
     5221 cttatttaat tctcctaaat acaaccctat aatgttgata tttaggatac ttttgtatat
     5281 aatacgcact caataggcat caaagtgcaa ttgctgcact aactcatgct tattaaacgc
     5341 actttaacaa tcaagagtat atataaaagc taagctgtgg tgcacctagc gccgaatcta
     5401 agtcaggttc gactctgcgc gcactacagc ttaaaaaatg tagccaaaat ccaaaatagc
     5461 gtcctgagga ggtacgccac cgaaactgaa atgtttgtgt tgagatctta gaaacaaaaa
     5521 accgcgcaat atcaatctga ttcatggagg ttcgtatgtc acacaaatct tctccttttt
     5581 ggagaatcgc aagcgccctt ttcttgcttg cgttgatcgt attgccgtcc attcaggtag
     5641 ctgctcaaag tacctggaca caggatgcct acgtcttcga ttccactggc aagctcagcg
     5701 cagatttcat cgcgcgtttt aacgccaagg tcaaagaaga aggttatccg gtctatctgg
     5761 ctgtcgttga cgagtcgctt ctgggcgaag gtttttatgc ctgggatgcc gctaaacgcg
     5821 atctttatga cactgggaaa atctcaggta gttttgtcat gatcgtcatc gcgccgttcg
     5881 taggtaaaga cagcccggtc tacaagttcg gcattaccga gatgatgcag ggtggggttt
     5941 attcaccact cgctgaacgc accatttctg gtcattccga aaaagccgga cctgaagttg
     6001 ccgcctggat tgataatgaa attcaaaacg gaagttggga aaaggcagcc attgacggta
     6061 tccatgaagt cgcagccgcc tgtcaggcac tgtcgcttgg gtcatctccg tcaagccagc
     6121 cagctcaaca agcgcctgca ccgacttctt cagaatccaa ggtcttcgtc gtcgaaaaga
     6181 accctatcga ctacagccgc gtctttttga ctcttatcgg tgccattgtt ggtatgctcg
     6241 tccttgtttt tggagggatg gcattttata ccgcaaatca ggcaagccgt aagataaagg
     6301 atgcacgtca tcgcgcagtc caaagccaga cagccgcagt caatctgatg accaggttcg
     6361 aagaagccgt ggaggatgca cttgagcgaa ttcagtttct tgaccttaaa gtcgctcccg
     6421 cgttaaccgc ccaacttcgt gctcaactga ccaccctgaa gggggacgtc tccggtccac
     6481 tgaccagttt ccagagtgta ggtgaacgtc tcgaacagcc tggcgctgaa gcttcagaag
     6541 atgtctacga tgaaatccgt cgtcaactcg aagcccagat cgtcatccct atgcgccggg
     6601 ccatggaaag tctcaaccaa atcgttgctg aaatcgaagg gttggagacc caggtggaga
     6661 ctgccccgca aggtgtagaa tctgcccgcc agaagattga gcaagctgaa caagccatcc
     6721 tggcccttga gactaatggg atgaaagttc ctaatctccg tcgggaattg gctgaagccc
     6781 aaggcttgtt cgttcgtgcc cagaagtctc tggttgcact ggacttgccc ctggccgtct
     6841 ctctcgctga aaaatgtttg gctgaagcag ttcaagccct tgaacttgcc caaaacttca
     6901 agaattgggt aaagggtttg tttgccgacc atgccgagat taccgcgcgt ctggaaccag
     6961 ttggcaaact tgttgccggt tcacgagaaa ctctcgaagc cttgtcgcac agttttgtcg
     7021 cttcgtcctg gaaagaactt aaggacaatc cgacccgcgc ccaacgcaac atcgagagag
     7081 cggtaaaact gctagccact gccactcaag cacttgaaac ccaggattgg gaacaagctg
     7141 accaggccat tgacgaagcc acgctttccc tgcaagagag cgaaatgctt tgccactcca
     7201 tctcagcacg tcaagaagag ttggaaaaag ccgtcaagca ggttcaaaaa ctcgtcgctg
     7261 acgtcactca ggcacttgcc tcagtcaagg cttatgtcgg gcaacatgat gccgacatcc
     7321 ctgaaagagc agaagaacgc ctcgaagtgg ctgaaagacg agctgcgcaa ctgactgaag
     7381 accttggcgc tgaaatgcct gactatttgg caatcaccaa actccttgcc gcagtcttaa
     7441 aagaggcagg agagattctc gcaaacagca aggatgacgt cgagaagatg tcccaggctg
     7501 agaatattgc ggaatcggag atcgctgatg caacacgggc agtaaagcgc gccgactcct
//
LOCUS       DDWP01000003           23533 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_5710, whole genome shotgun sequence.
ACCESSION   DDWP01000003 DDWP01000000
VERSION     DDWP01000003.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 23533)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 23533)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23533
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_5710"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    22775..22901
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7873 a         3992 c         4903 g         6636 t
ORIGIN      
        1 cgaaaacaat caaagcagca atttaacccg tctttttcta gacgggtttt ttgtgtcgta
       61 taatcagtta tatgaaaatt gaaaaaaaga aaataactga gaaagtaagt gggcataaaa
      121 atgaattttt ggagtttttg aaaaactata atgtactaga attatcaatt ggagtagtga
      181 tcggtggagc attaaaagac ctgacagctt caatagttga tggattaata atgccgataa
      241 ttgggatatt aacacccact ggatcgtgga aaacaatcgc ttttactatc gctggatcag
      301 aatttaaaat aggaaatttg atctctagtg taatcaactt tttaataatc gctttgtttg
      361 tattcatcgt cattaacaag atcctcagaa tagaaaagat tgacaagaaa aaataattga
      421 tctagactga agttagttaa aattttattt aataaaaatg tcaaaaaata caggtggaat
      481 gtttcttgct ggaattattg gtgctctagc tggtgccgtt ggcggactat taatggctcc
      541 acaatcaggt aaagaaactc gtgaagcaat ttctaaatta gctttacaaa tctctaaaaa
      601 tattaaaaca gaaactactg aaactaaaaa gagagtagct gatatttttg gaaaagcaaa
      661 cgaagaagtt atcgataaat ataatgaagt taaaaataca gtggttagta aagtagctac
      721 tattaaaact gctggtgaag aaatcaacaa agagaaatac ggtaaattgg tagaagatgt
      781 ggtggctgaa tttaaagaag atttatcttc aactaagtca gctgctgaaa agatcgttgt
      841 ttacctaaag aaagactggg aaaaagtaaa aaaagctatt gcctaaaggt gagttgattt
      901 tagttaattt tgtgattata atccaatact tatttattaa gtattgttat ggttgcaaaa
      961 aaccctactc atcttatatt tactcttgaa aatgaggcag tagctagagt ttgtaataaa
     1021 gttttaccta acggacagat tgtgcaggtg tctgatggag ttgttgttga agatacaaca
     1081 gaactagcga gggtagttgc gactatggct gagtctgccg gcagacaaaa acttgttggg
     1141 gttgatggaa ctaagaagaa aggtgctgag agataaggtc attaagggat ttgagagagt
     1201 tgtcgtcggc aatactagtg gctaggacgg ctttggtttt tttgactaat tttgtgagag
     1261 ttttctcacc gagcaaatca gatttggtaa tgatgattag ctcaggtttg ttggctaagg
     1321 cagagctgta attatctaat tcgtgacgaa cagttttgta atcggctaaa atatcttctg
     1381 attcggctga caagcaatgc acaagtatct ttgtcctctc gatatgttta agaaatttgt
     1441 atccaagacc tttaccttct gaagcgcctt caattaatcc tgggatatcg gcaataatgt
     1501 gaccccctcg agtaacacca agatttggct ctagagtggt aaaggcatag ttagccacgc
     1561 gagcatgggc ggtagttagt tcatttaaaa gactggtttt gccggcgtta ggtagaccaa
     1621 ttaaaccaat atcagcaatt aactttaact ggatatgaag ttgacggacg gggggcacgg
     1681 tgcccatttg atgttcttgt ggtgtctgat tagtagaaga acgataatga aaattacctt
     1741 taccacctcg accaccttta gcagccataa ttgtctgccc ggcagtttca agctcaacac
     1801 tagtaccatt gtcataagta acaacagaac cgatggggat ttctataaaa agactttcac
     1861 cttttttacc ggaacattgg ttcggaccac ctttttcacc atcaggagcg tgaaattttt
     1921 tctcatgacg gaactgatga agtttactga tatcggaaac agctcgaaaa tagacagatc
     1981 caccattgcc tccattacca ccatccgggc caccttttgg tcgccaacgg tcggtataga
     2041 aatggactaa accatcccct cctttaccag cttgaatagt tattgaaaca tcgtcgatta
     2101 acataagatt ggtacgttag taaatattaa gtattttacc attatttttc aatgatttat
     2161 aagtccaacc taagatctca gtcttgccac tcaacatctc gaggggtaat caatctgata
     2221 agtcctattc tgttgtttaa tcgagctact ttaaccaagg ctcctaccac ttggtcgtcg
     2281 tcgtcaacaa cgggagaacc gctcatcccg ggtttaattg ccttacttga ataaattact
     2341 aaatgcctgc ttggatcaca tccttcggca aggttgcgga tgattggttc actatcatta
     2401 acgattgcaa cgccgtaagc ttttcgtggt aaaaactcgc ctgatggtgt atgtatagtt
     2461 aacaactcat gatgtgccac taacccttga cgaagagaca acccgggacc acgtactgga
     2521 ccagcaacac acaagtcatc acctctcgaa ttaaatccca atgagatgcc cttatttgta
     2581 ccaggaatag caacatctgc cctactagca gtgacatgtt ctggggcaac tatgattgac
     2641 ctacctttac cagcctgcac tctgattcca acaccttcct ttctggggcc aaatactgac
     2701 aacaaaaatt ctttcatgaa caaatattat actataggat agtccgcttt ggcaacatca
     2761 agattagtcc acaataagtt gaccacaggc ggagtcaata gattgaccaa gactgtggcg
     2821 aacagaaaag ttgacaaaag ctgccgataa ttctctttca aaaaaactta gtttgtcaga
     2881 aggagtaagc ccgcctttaa taggattaag agggatcaaa tttaaataaa ataatttatt
     2941 tgattttata aattttatta ataaatcaag atgcttggga ctatcattaa catctttggc
     3001 tagaagatat tcaaaaaata cctgtctgtt ggtagttttt gtatagtaat caagtgattt
     3061 ctttagttca gttaaagagt gttgcttggc aataggcatc atgttttctc tggtagcctg
     3121 gtcggcgcta tgaagtgaga tagcaagatt gatttcagta ttaagattgg caaagtcgat
     3181 aatgcgagta acgacaccgg cagtagaaat acttatcttg cgagagccaa taactaaatt
     3241 ggttttgatt gattcgagag caggaagtag attatcccag tttagaaatg gttcacccat
     3301 acccataaag acaacgcgac tgactttgtg gtcatgtttc caaaagagga tttgatcaac
     3361 tatctcgtcg acggtaagat tgcgtttaaa acccattttg ccggtagcac aaaaagtaca
     3421 accaagtgga caaccaactt gtgaactgac acaaactgtc tgccattgtc cgtagtccat
     3481 taaaacagtt tcgatttgtg aaccatcagc taaaacgagc ctggcctttt tagtggattc
     3541 actttcaaga atgagattgg atttaacgga gtaaagtgag aaattttcag caagcttttg
     3601 gcgcagatcc aaagacaagt cggtcatgtc agaaaaagaa gaatagcgac cggaatagta
     3661 gttgagtttt atttgtcgaa ggcgaaaagc aggctgatta ttttgtacca aaaattcgga
     3721 taattttgag aagtccacta tctattttag cacagcaagg taatctgctt tttgatggaa
     3781 accgacgatg gtgtctttaa cttgaccgtc ttcaaagata actacggtag gaatggagct
     3841 gacaccgtaa ctagaggcta tttctggata ggaatcaacg tcaactttaa ctatttttaa
     3901 attgtcaatt tcctggctaa tttcatcaat aattggagcg agcattttac agggaccaca
     3961 ccactcggcc caaaagtcaa ctaaaacttt gcctttgata tcgagaactt ctgccttaaa
     4021 atccatagtt aatccatctt tttaacaagt ttggcggagt gaactatctg gcgaataaca
     4081 ccatcaggac cttcgatttg gttagtgccc gaaagagaaa cgtattggga caaataatca
     4141 gagagagcaa ctttaagctc tttcatttct gattgaattt ctttaacttt ctcgacaacg
     4201 gtagatgcgg caggtgactt gagaactttt tgtttggcaa gcattttaac tttacctaac
     4261 ttagtagcct cttggttggc gagagtgtat tcttcattgc tctcaagcat gccagagagc
     4321 atatcttttt ggacattgag atctttttgg agttcagtca atttagcatc gtggctattg
     4381 actaaagact caatattaag gaggttggtg acagcactgt tttgattatc ggtcatacgt
     4441 ttaatacttt aacaattgag aaagtcttgt tcaatggcat aaagttggtc taaagagctc
     4501 aaactgacca actggctacg aagttgtttg gcttgggggt gacctgaagc ataaattaag
     4561 aaatgtttac gcaaactatc aaaaagtcgg tggggaaata ttctattaaa atgataagca
     4621 tgacgaacca tggcatgaaa cttatcttcg taactcccct ctttgtctgc cgaaaaaatc
     4681 caaggattac cgagggctgc tcgaccaatc agcacgccgt cggctccaaa ctcctgacca
     4741 tactgaatgg cttgagcagg agaagtaaca tcaccattac caaacagctt ggttgaagtt
     4801 tgatgagcca tagtggctgc ttttttgatt tcctgccagg cagctagtcc ggagtagcct
     4861 tgttttaggg tacgaccatg gagagaaata aaatcaagat cgtgagataa aagatgaggt
     4921 atccaagcgt cgacgacgga ttcggtaata ccgacacgag ttttaacact gaaagttggt
     4981 ggttgagcac cgatgttggg tatatgggaa gagtatttca ggttgcgagc aataacggca
     5041 tgagtttttt gatttagttt tagatcatca atagtgactt ttttgttaaa ccaatcatcg
     5101 ataccagagc gggcagcttt gataatttcc gaggccaatt ctggcttggc gataagagca
     5161 gctccgctac cattttgagt gacagttttg gcaggacaac ccatgttaat atctactccg
     5221 tcaaatccaa gatggcacaa aacaatagca gatttataaa aactctcagg atctttacca
     5281 aatatctgac caattattgg ccgctctaat ggggaataaa gtaagacatc atataagcca
     5341 acacctccgc gcgaaagacc ttcggcactg acgaattcgg taaaaataac atctggttta
     5401 ctaatttcgg cctgaacaag acggaaaggt tcatcggtgt aaccatccat aggggaaaga
     5461 ccaatgataa atttatcatt gaaccattta tcatttacca tttttattta cccctagtac
     5521 gcttagcaaa cttggaaccg acttttgaat ggtgagtgcg agagtcgtaa gttgattgtc
     5581 gagtcttacc aaaaactggt ttgcgtttga atggggccga ggaggcagaa gaaccatgct
     5641 tagggccagc agtaggagca gtcttgggaa acttagcata acgagcttct ttacgggtaa
     5701 cagcttcttg gtgacgagct tgaccctctt cggttttaaa tgggtcgggc tcagcatagt
     5761 caacgattat agttcgatca atatcgattt tgtagttgtg taatttgatc ttggcactaa
     5821 tagcagaatc aagattgtcg aattcgacaa aacctaaacc tcggctttta ccccacttag
     5881 tgtgcattat ctttaatgaa acaactctgc caaaaggtgc aaatagatcg agtagctcac
     5941 cttcagtaat tcggtaaggg atacccccaa cgaaaagtcg agtagatggg tctttattta
     6001 atttttccat aatgtattat attatagttt atgcttaaat acgaaaagat ggttttggga
     6061 gagatgcaga ccaatagcta tcttctctgg gatgaagata gcaaggaagc aatggtgata
     6121 gatccggcag atgatggggt ggaaatagcc caagaactac agaaactaga cctcaagcca
     6181 atagtaatag cctgtactca tggacatttt gatcatttac tgggagcact agacctgatg
     6241 ttgatattta agataccact gtgtttgagt aaacttgatg cttttttact tgaaagactg
     6301 ggagaaacag ctgaacactt cttggggcat aaaataaatg taccgaatat aaaaaagatt
     6361 gatattgatt ttaataaagt agtatcaata cgattgggtg ataaaaaaat cataccaatc
     6421 aagactcctg gtcatactcc cgggagcgta tcaattcact gccctagtga taagctccta
     6481 tttaccggcg acactttgtt tgctaatggg tttctgggag aaaccagtca caaatactct
     6541 tctcttctag atatgagaaa aagcttgaag agattattgg aactaccgga ggatacgcta
     6601 gtgttgtcgg gacacgggga cgaagatacg attggaaact gtaaagttac tcttgagaat
     6661 tgagtgattt gatagtttca tcgaagttaa caacgttatc ctgcttgatg ctctcaagaa
     6721 gctcgaccgc caattcgttg caataagctt caatagcagt tcgttgttct gaagttaaat
     6781 ttagttgttt agctagctga tcgaatgttt gcatggagta gtttaacatt ttgatcactc
     6841 ttgtgtctta aaaaagaggt tgacggttaa aaccatagct aaactcatag caaagagata
     6901 ggcaatatta agtgaaacaa gagtagagcc aattgtaaaa ataaatattt gtaacaagct
     6961 attgagaaaa ctggcgagag ataaagtagt ggcacgattg taggaaaaac tgtttttatt
     7021 gaatagatcg gaaaaataag gccagcgaat agtttcagag gctaggatta tgtaatagag
     7081 aaataaacta aaagcccagt tcttaaaaag tatctggtta agtaagaaca aactaacaac
     7141 gataaggttg accaaattaa gatagttaat gattctggca ctggagaaat ttcgtaacca
     7201 gtaatgagaa ttaagattaa agatgaaaat agttaattga ttaaaagatg taaccaggcc
     7261 tataaacaga attgggatac cgaggccata aaaataatcg gagctgatcc gccaaatcgc
     7321 aaagctagag acaaagacta gactacgatt gagaattaaa cttaatagtt taggattgtg
     7381 actcagcagt ttaacggcgt caagcataac tcccgattca atcttttcta ctttaagatg
     7441 atgttctggc tcaacgattt ttgaagctaa aaatatggcg attagattgg caataatgtc
     7501 gatactgata atgaggttga actgccaatc agttaagttc ttggctaata aaacggcaat
     7561 tattggagta atgattttga aaattctttg accggaataa aattgcccca acttctttaa
     7621 actattggtt tcttcaccta attctttggc agtgtcatag actagggctt catctgtgcc
     7681 tgacattatc gaatatgcta aagcatataa agaagtagcg acaagaaagg cagaaaaact
     7741 atgggcgttt atatttagaa accaataaat tgctccgatg aacaaagaaa ttatgatcaa
     7801 ttttttacga ctccacttat cagctagata gctggatgga atctcggtga gaaccgagac
     7861 ggcagcaaag gcaattgcta aatagaagat ctgactaagg tttaacccac ggtaaacata
     7921 aaaaatagta ctgacgacat taagaacttt tacctcagtt aaagcttgaa cccaaaacat
     7981 gagtttgaag ttttttttca aaccattctt tttgtcataa accatggaag agtattatat
     8041 aatatgtcac tctaccccgc cctcctacgc tgaagctacg gaggataaat cggtgttttg
     8101 gtagaataga cttacgatac cccgtcgtct aatggtagga caatcggctc tgaaccgatt
     8161 aattgtggtt caaatccatg cggggtaact gattcgactc acttcgttcg ctcacagtta
     8221 taaccatctt gtgagtcgat catcctgagc gagcccattc gataaactca ggacgagtcg
     8281 aaggaaatat gtattacgtc tatattttgc gtagcataga caacagttta tatatcggtc
     8341 aaacaaatga tttgaccaaa cgaactcatg aacacatttt tcatctggat aaggcggcga
     8401 agtttacaaa agaacataat gactttaaat tagtatactc cgaagaattt gatactaggc
     8461 taaaatcaat gcgaagagaa aaacaactga aagggtggac aagggctaaa aaagaggctt
     8521 tgatttctga tgatctggaa ttattaaaga agttataata gtgaatgaat aaattcaaca
     8581 aaatatcgac gatcaatatt cttaatctgc cgtctgaggt gatggagcag atagaaaatt
     8641 attcaaatca agaggttgtt ttttacgata ccgacagtca atcagaagaa gaaacgattg
     8701 aaagaattgg aaatgctgat gcggtgttag gatcttggaa ctcaactata actaaaaagg
     8761 ttcttgatac ttgtccaaac ttaaagtaca tcgggatttg tggaactagt ttaactaaag
     8821 tcgacgtcga ggaagtaaag aagcacaata tccagctaaa aaatgtaata gattatggtg
     8881 acgaagcgac agccgagttt atttttgctc aattacttaa tttgcatcgt gggtttggta
     8941 aatatcaact cgattcaata ccgactgagc ttaacggcaa aacactgggg ataattggac
     9001 tgggagctgt tggacaacag gtggctcgat tagcacttgg ttttaatatg aaggtgatct
     9061 attactctcg aacacaaaat agcgagtggg aaaataaagg actggtatat caagagctta
     9121 atcaaatact gtcaacttca gatgttattt caatcagtgt gccaagaaat ctaaatataa
     9181 tcggtaaaac agaatttgac ttaataaaac caaaaacagt tctagtaaat actgctatcg
     9241 ggacagtttt tgacttacca agttttgtag aatggattgg caaacgtcaa aatttcttta
     9301 ttgttgataa tcaggaatac attgatcaaa ttaaggactt accgaatgta attgctctac
     9361 caattactgc cggtaaaaca agagagtcgg tagataggtt gggtaaaaaa gtgattgata
     9421 atattaaaag ttactttcta agtaactagc ttttgttcga tagtcattcc agcaagctaa
     9481 cagatcaggc aaagatgagg ttagagataa gaactgatgt caacttcttt ttgatcgacg
     9541 agaatcttgc aggaggcctt tagtttcttt gacacggcga tacctgactt aatcaccttc
     9601 ttaaaaaaac tacgctcttc aagcaactct atggcgttac ttattttatt tgtagtaatt
     9661 agtatcttga aacagccttc atcatattca aagttaatat caacaatatc gctataaata
     9721 agatagccaa acttcttctc ataatctttc tctaatcttt tttgagaaaa gtccattttg
     9781 tcggcaaaac ataccaagtt agtgaacaga gatagctcgt caagatctgt ttcttgatcg
     9841 tgtttttcga tagccaacaa tatttcattc aaactatctt tcggtatctc gtttgggaga
     9901 tactcttgag aaaaaagctt agcccttaac tcatgatttt ctcgaccact agcctgacca
     9961 atgtcatgta gcgcacaggc tatttgcaaa tattgttttt gattgtcaac tatacctaat
    10021 atatcggcta ttttacctac tatctcggct acatttgctg cgtgttggag accgtgacta
    10081 aaggggtaaa tattttgagc atctatcttt gtatactcat caattatctt ttggtcagcc
    10141 aatatactct tgtaagtaat catggttgat tataacctac gctttttcaa ccagaactta
    10201 tggtgtaaaa tcatacccat ggacaaagca aaaatcctca ttaccggtgc gagtggttat
    10261 attggagcga gactctttag agacttgtca aaaaactact tggtgtcggg gacatattac
    10321 aagtcaattg ctaatgacgg cttagtgaaa atggatgttt cagatattga atcagtacga
    10381 gatgtattgt cacaactcca accagagttt gttgtccata acgctgcttt tcctgtaagt
    10441 ccacaaaatg atgaacaaaa aacaatagtt gaaaatgtta actttctagg gactgataat
    10501 atagtaaaag tagctaatga gatcggggct aaagttattt ttatatcctc agcagtggcg
    10561 ttaaataaaa atgattttta tggccaatca aaagcatacg gtgagtctgc ctgtcaaagc
    10621 gcaaaagcag gcagcttaat aattagacca cacacggttt ttggctttag cccaaatatg
    10681 ctaaacgata ggtcttataa tcgcctgtta aaaaacatat atttaaaaac accagctgta
    10741 tacgatactt catggaaatt tgaacctacc tacattggcc atttatcaaa tgtgactcaa
    10801 caatatgttg agggaaaagt tgattgcaag gagattaatg tggcctttga tgtgttgaaa
    10861 tcaaaatacg atattggaaa agacgtgttg ggtcattttg gtatcgacgt actaccgact
    10921 gatgataaat cttcccgccc aacgatcaaa cttgaattag atgacttcgt ggcattgaat
    10981 ttaccaataa gagattatga cgagatgata tcggtgatgg ttgaagaaac tagggctatt
    11041 ggggatagtt atccaattaa nntatgaaga aattgatgag ttatgataga taccctcatg
    11101 ggaaaagaaa agctcacatg gaggtggaaa taaaatgtac ggattgcgca accactatct
    11161 gccaatacga caaagagggg tatggaccaa taggtaagat tatttttaag tctatattgg
    11221 gtgagagtga aataaaagat aaaaaagaac ttgaatgtca gaaatgcgaa aaactacttg
    11281 ggaaaaaagt gatagcattt gaaaacaaag aaaaagtaga gtttttcaaa atgtaccctg
    11341 gagtgatttt ctataaagtc ttgcagaagg gaagaaaata agaatggtag taaatataaa
    11401 agaattaatt gataaagtta aaggtctagg tggagacatt gtcctaactg gcggttgttt
    11461 tgatgtatta cacataggtc atatcgtatt tctcaataaa tcaaaagaac acggcaaaac
    11521 attggtagta ttgcttgagt ctgacgaaag aataaaaaga ttaaaggggg aaaatagacc
    11581 gattaataat caaaatgatc gagcggaaat tttagtcaat ctgaaaagtg ttgatgtggt
    11641 ggtcaagctc cctccagaag atatcgacta ctcacaagtc ataaaattaa ttggaccaaa
    11701 agtgattaca gcaaccgaag gtgataaaat tatggagata aaacgacaac aagcgaaaga
    11761 tgtcggagca gaactgaaaa cagttcaatt tgtaccagat aaatcaacga caaaaataat
    11821 tgaattatta ctttaaacaa atatggacgt atttgaaata cacaaacgaa aaacaggaaa
    11881 gattgaggtt aactcgacag tagaagttaa tgacagggaa acattatcta atatatatac
    11941 ccctaatgta ggtaagatat gtatggaaat taaggacaag ccagaaacag tccgagacta
    12001 caccatggcg ggaaaaatga ttgcggtgat ctctgatggg acagcagtgc ttggctttgg
    12061 agatatcggt ccagcggcag cactacccgt aatggagggg aaatgtgtga tatttaaaga
    12121 atttgctggg ttaaatgcat ttcctctgtg tttgtcagaa aaggatccag aagaaataat
    12181 taaaactgtt aagaggttgg cagttaattt tgcagcaata aacttggagg atatatcggc
    12241 accaaggtgt tttgaaattg aaagcagatt gaatgacgag ctggatatac caatagttca
    12301 cgatgaccaa catggaacag ccatagtaac cttagctgcc ctgatgggag caataaagct
    12361 aactggcaaa gaaaaggtta aaatcgttgt ctctggagcc ggagcggccg gcaatgcaat
    12421 tacgaaatta ttagtggcag caaaagatga gatatccatt ggtgatatag ggatatatga
    12481 ttctaagggc ttggtggcaa gtgatagaag tgatcttgat aaatataaaa tggagctagc
    12541 aaaaataact ggacaagaaa aaacaaagtc tattaaagaa ggtatggtgg gagcggatat
    12601 atttatcggg gtatctgtag ctaacgccat cgatatagaa atggtgaaaa gtatgaatga
    12661 aaactcgata gtatttgcta tggctaatcc gacgccagag ataatgccag atgaagctat
    12721 aagaggtggg gcgttgatcg tagccaccgg aagatcagat atccccaacc aaataaacaa
    12781 tgctttggcc tatcctggag tattcaaagg actcttggag atgaaagcta acaagataac
    12841 cactgagatg aaattgttgg cagcgaaagc gatatatgaa tataacttga atcaactatc
    12901 caacgtacag ctattgccaa gcatattaga caagaaggtg ccggggatta ttgctcaagc
    12961 actgcagagg aagtagcaat aaattgtcga atctctgagt aatcatttag tcctgatcga
    13021 gggataagaa ttgattgacg actactgcta taagattggg taagaaaatt cttatcatta
    13081 attgatatcg aattaatgtc gtattttgaa aattcgagtg ctcgaaaaag atacttttcc
    13141 aagtctgaaa cagacaggtc actgcgaaga tgtgatttta atgtattaaa aattggaatg
    13201 attctaggga aaatattaat attcagtgct ttatcgcgga cagctaaaag aacttgacgt
    13261 tgacggttgc tacgattgaa atctccacca tcaaactcag aatgacggga acgggcgtat
    13321 ttcaaggcgg tagcaccatc aagatgagta gtgccaatgt tcataactag ttgttcataa
    13381 cggcaggtaa actgatggtc taatttctct cctgacatgg tggctgacaa agcagtgacc
    13441 tcctctggac ttttgccaca agtatcagtg gcaccgacat caagtgggta aaataaatct
    13501 acaaaagggc gggtaatctt tagatcaact ccaccaagga tgtcgacgac ttttataaaa
    13561 ccattgaagt ctatggcaat atgatgctca atagtttcgc ccgaaatact ggcaacaaca
    13621 ctcttgagtg cttcggaacc agacaaaggt aaaatagaat tgatcttggt gaatttatcg
    13681 ggagagactt ccacccagag gtcacgagga attgaaatta aatctatttg ttgatcgtga
    13741 ggtcggatac gagaaagaat aattgaatca gtaagaccac caccatcgtg attggctcca
    13801 gcatagccta aaagtagaat agaaaaagat cggtctgggt cgggggttgg ggtaggagag
    13861 ggcaaaacaa cagtgggtgt agtgggtggt aatttataat tcttattttt aagtttataa
    13921 ataaaaaaag atattaagac aacaataaaa actgcggaaa aaatagatat gagatagaga
    13981 aatatgggtt tacggaacat ggtcaggtct tgatatattg gatgtcggca ccaagggcac
    14041 gaagacggtc ggcaagcctc tcataacccc tttcaataaa ctccgtacct tctatggtcg
    14101 acaagccttc agcagttaat ccggccaaag tagccatagc cccagcccta agatcactga
    14161 ccgacagatg gcaaggtttt aacttacttg gtccgtatat tttaacccca tggaagtatt
    14221 ccggacgatc actctctgga ttaaattcat aatagtcctt ggggtctttg acaatcggat
    14281 tgaagaactt ggtcttggca cccatatcat ttaaagtttt gatatgttga aatcgataag
    14341 ggaagactct ctcaaccaca ctactgcaac caacagcttg agtgagcact agactaaaaa
    14401 cggcctgcca atcggtcatg aatcctggtt ctgcttgagt ttctatgtcg atagcagtaa
    14461 gtccaccatt ataaataata tctacttcat catgaccacg agtgaccttg gcacccattt
    14521 tttcgatagt ttgaagaaaa gtctcaatga ttctgggtcg aactcgaaga atattaatgt
    14581 ggcctttagt agataaagca gcacaagcaa aggtaactgc ttcgtttctg tcggcaatga
    14641 cttgatgctc ggtaccatgc aaagaagaaa ctccgttaat aataattgtt tttggattag
    14701 ttgggtcacg tttgatctgg gcacccatag aattaagcat acaaatgagg tcgtctatct
    14761 ctggttcaat ggcagagttg ccaatgatac tttcccctgg agttaaggca caggtcatta
    14821 agataacttc agtgactgta tgtgatggtt tgggaaagtc ataatggaca ggtttgagac
    14881 cagtagtggt aaaacgaaga tgatcatcaa attcttcaat attgatattc atttgacgat
    14941 aacaatcaaa aagtcgatcg agaggtcgag caccaagttt gtcaccgcca ggtaaaggaa
    15001 taattgcctc ccctactcga gccaataatg gggcggcaaa cataaaagaa gatcgtgact
    15061 tatcaccagt gccactaggc accttagtac tagttatctt ggaagtggta atttcaatgg
    15121 agtgattgcc aattagggat atctgtgaac ccatactttt ggcgatactt tcggtaagat
    15181 gaatatcgga tatgtcgggc agattaagta gcttagtggt agaaggagaa aggagtgaag
    15241 caattacttg cttaaaactg gcattttttg cgccacgaat agaaacctcg ccccgaagtg
    15301 gattaccacc attaattaag tatcgtgaca tttgtaaaga aataatatca cactatagcc
    15361 taatgagatg gtaaaagaag aaaccttagc gaccgatatg ttcagctaga tttcaattgt
    15421 cttaaaaaag tattataatc tgtcacttta tttatttgtt ggaatccatc agtaaacatt
    15481 attaattaat agtgttgtat ttggaaaaag gcagtatatg aaatattgta caaaatgcat
    15541 attaaatgaa cacacaccat ttatatcttt tgactgcaaa ggagaatgta gttactgtca
    15601 tgaacaggaa accatagagt cagacaaaga ttttcatctt tatacaaata aatcagattt
    15661 taataaacta ctcgctgaag aaagaggcaa gaataaatat gactgtattg ttgcgtgtag
    15721 tggcggaaaa gacagtgtta tggctctgta tgccatcaaa aaaatactgc atctgaatcc
    15781 tctagcgatt atcttttcta atcagttcct agttaaagga atggtagaaa atgttcagaa
    15841 tgctgctgag aagcttggcg tagattggag actatatcat tataatgaag taaaaaaggg
    15901 gtttgaacac tttctaaaaa gtgaattcag aaaacagata agtttatgcg atgcatgtca
    15961 gctatttatc tcacctataa aaattgcaaa gaaaattgcc agagaagagg gtgttaaaat
    16021 tgtctttacc ggtgcaacaa tggcacaaag aataggtgcc atgacaaaaa acagcacaat
    16081 tccgactcac caaaagtatt ctgaaagata ccggactgtc atgaataatt taaaaaaata
    16141 tctagggcag tttaatgaac taaaagatat tttatctgag gtagatgatg atggctcgat
    16201 agtcgtaact tctccctggc attacttaaa tcaagaaagg ttggaggcgg actccatcct
    16261 caacagtctt ggttggaaac aaataaaaca tagttttcca ccaaaatcga ccaattgtga
    16321 actgtcgttc ttaactgcat atttgggtcg aaagtacaaa gttgccaatt acgacataac
    16381 ttttagtcgt ttaattcgat ttggagaaat ggaccgacaa gaggcgatga aaaggttcaa
    16441 aaaaagaatt cccccttctc tattaaaaaa gacactatca aaattcaata ttgacgtaga
    16501 tacactctaa tttcatcact tactaaccaa tataagtctg tcagaatggt atgtaacaga
    16561 tacttacttg accccttgtg aatataatcg ttgggtgtat aatccactaa ttttagtatg
    16621 aaaccaatca actctattca ctggagtaaa ggtaatttta ataagttttt tcaggaagat
    16681 ttatacggga gatttgattc agactctaat ataatcctta gtcgaggaag ctgggaggac
    16741 aaccatgtcg tatatcctaa attattttct ttttgtttaa actattccct cgataagcat
    16801 tgggccggtt attctgattc tctaggccac aacaattcgt ttgaggctat ttgtaaattt
    16861 gctaacttgt attttaatga cagtcggtac tactacaaga acgacaacgt ggcgattaca
    16921 atgggtaacg tgttcgcctt aaatcaaata ttttgccacc tcaagactct aataccaaaa
    16981 gctgaactaa tagcccttaa acctttttat ccgtcaatac taaaatctgc ttcgagaaac
    17041 tttaaaaagg tgaagttcat ttcttcgtta gatacggagg atgaaatatt gcttaatatt
    17101 gcggccaaat tagaatcaaa atcttcaaaa attttactac ttaccaatgc tatcggggtc
    17161 gaaggcagga tcttttctga aattttttgg caaggggtct taaaaattat caacaacaga
    17221 gatgtatatt tgattattga tgaaggaatg tggtttgaac ccctaaaata tccacaagag
    17281 ataaataata gcaatgtaat tagagttgtt agtttttcaa aaaaatttgg tatccctgga
    17341 atgaagttgg gattcatgct ggcaagcaaa gaatacttga gcaaatacta cgattgggcc
    17401 tcttccaact atggtggacc tccaagcata ttctttttac tgatcgagtt catatgcttt
    17461 tttgaataca tccagcattc gagtaccgac caaacaaaag aattaatttt tctatctgaa
    17521 agatatggca ttgaactcga aaaactggca gaactatatt acgatttcgt tgactctaca
    17581 aaacagaatc aatataaact tggttccaat caaaaaacat tgcgaaaatg gttggatgaa
    17641 aacaaaacgt acattgaagc tgcttacgat tttggtggaa ttaacgtact tatactccca
    17701 aagatagctg gttctagcta caaccttttc ttaagtctta taaagaatgg ggcatctata
    17761 tttcctggtg cttgcattgg tgacgacacg gattctctgt ttcgtgtcac tttattagag
    17821 agtcaggaaa acttctgttt aggtctcaaa ttattaggta aaactctaaa tgaatacaaa
    17881 tagatttgtt caaaaactac tgaacttcag caggcctaaa aggctatacg accaggacca
    17941 tgtcttattg caagaaagat tcgagccagc ttcctggtca aagagcttag acacgcagct
    18001 agcgagtatc aagaaattac taaaaaatgc aaaaatggac aagggatact acaacagcaa
    18061 aaagaaagtc cgtgtgccga gagggtacac aagggtaatt gtgcccaaga tgtcgtatct
    18121 ggcaaaaatg tacaaggttg aagatccata tgctgaaatc ggagttttag tcgaccaagt
    18181 gtgtgagcta ttgattgtga atcgtgacgg taattttacc cactatcgta agggtcagct
    18241 tggtccagat agaattcagt gtattcaaaa aatgacattc cctcgaattg atctagaact
    18301 gaatgttcct ggggacttat tagttttaga tgtagatatg ggtaatcatt atgctgggtg
    18361 gactccgaga agggctcgcg aagatatatc cttcagagat gacataatgg cgttgtctgg
    18421 agttgactta tgctggattg tcttaataaa ccccaacaga tttcaaagaa acgtcgactt
    18481 gtcagtagat tcagtttttg aagagtatct gtcaacggat agaggctggg taacgaactt
    18541 attcatgtat catcgtggtg gaaaattaga atttggttac cgatggaatc ggagggcata
    18601 ttttgactgt ggtgctgcaa tcgcaaaaat aaattaagta ttgttatgta ttgttactac
    18661 accgaccacc attgctgttc aaatgaactt gagttagcta aactgagcct tgaacacaaa
    18721 aacaaaccgt tcaatccaaa gctgggtatg gcactgctta cttgccaccg agttgaatat
    18781 tatccatcag aagaactgtc atccgtgaat ctcaaagaac taccaagcag tcatcgatat
    18841 atttcggata gtcttgaggc caaaagaaga ctactgagaa tagctactgg attggaatca
    18901 aagatccttg gcgaaaaagc catttatttc caagttgagg gggcaatcaa ctcgttttta
    18961 agtgcttttc caaaaaaaaa tggtttcact gcaatattag cttatgccaa aagtattcgg
    19021 gagaacttta acttctatgc cccagctcac ggagggttaa tttatgacta cattagtcaa
    19081 ggtgagtcag atattgctat tctaatcggg gcaggcatgt taaaccaatc aattgttaga
    19141 tctaatagtc gtaataaaaa atactcaaaa atattattag taactcggga tacaaaaaga
    19201 gcaaagaagc gtttaagagg ataccttgaa ggtgttgaaa ttctaaagcc agatatgttg
    19261 cgtgactgta atctatctca aaaatttgat cttattatcg ccacagacaa tattgagtca
    19321 agctatgaaa tgcagatcaa gaatcttgag actagcaagc tctgtaacta tgttgttgat
    19381 atctcttctt gtccactaac ctccttaaat tcaagctctg agatgtatta ctcactatat
    19441 actcgaaaca cggagattct tgttaagaag tcaaacttgt ttttaagaaa aaaaatcgca
    19501 ataattaata gttacattaa taaattagaa ctctagttaa gcttatggac aaacaaacta
    19561 tctaccaaat ccaggaggaa acaaaaaagg tgttacctga aatatttggc gaaaacatgc
    19621 tatttggatt tatttgtggt ggtttttcaa aaggatatgc caatgaggat cacgatatcg
    19681 atacttttat ctgtgtgaaa gagtcagtta gtagagaggt agaaaaaaag tatttagatt
    19741 ggtattttga tattcataag agatttggta ttaaggctga ctacgactat cccggagagg
    19801 tcgttgtcct agaaaggctg cttctaacaa ttgagattgt taaatcactc aagttgaccc
    19861 tgaaaattga cgaggtaaga attaagaaag ctattatctg gacagacatg ataactagtt
    19921 atacagcagc tgaatgtggt agtgacctta agctcctgta tcagataaaa gaggaataca
    19981 aacattatcc tcagcagtgg aaagaagagg tgcttgcact aatcacacca gaagaaaggg
    20041 aagaatggaa agataaaagc catctcttaa ttatggaacg ttttatgaag tacccaaaat
    20101 acgatggtaa aggccttgag aaaatatatg gccaaataat ttagtttcga atcagcccct
    20161 tcactgcatt tacttatgtc gtttgttgtg atattatagg actatgatta ttaaaagggg
    20221 acgcgagtat attatcgtat ctgacccaac tgatcaatac aaacaagaga tagatactgg
    20281 gcagtattca gaccttccgc ctactatgtc gagatttaca cgacaacagc gacaacaata
    20341 tgtgggtcta caattattag cacggcatga acaagaagaa caaatggctg aaaacagaaa
    20401 aaaaatagcc caagctgcag aaatggcacg agacggattt attagaggca tagctccgac
    20461 tagatttggt aaggctactg aagatcgggg tttataatta cggaactccc aactgtgtca
    20521 taaagatagg tggtaccgtc ttaaagatct aacgtgtttg tgtttacgcc cttcgacaac
    20581 catcaactat aacttgatgt atagttaact aatgagaagg acaaaaatag tggcaactgt
    20641 aggcccagca agtgaaaaag aggaagtgat aatcgacctg ttaaaagccg gtgtcaatgt
    20701 ttttcgattt aacatgaaat atggtgaaga tggctggcac aattcaagga taaaaataat
    20761 aagagagaat gctgagaaac taaaattgag agtggcaata ttggtggacg tgccgagcaa
    20821 ggactttaaa aaccagatcg aagactttga ttggctagcc ttgtcgtatc taaaaggcaa
    20881 ggaagatgta gtgacactta aaaaaagatt tgccggaaaa ggcataaaag taatggccaa
    20941 aatcgagaat gcaaaggcga ttcaaaacct agagtcgata gctaaagagg ctgacgggct
    21001 gatggtggca agaggagatc tgggacgaga gacaccaata gaagaattgg ccttctttca
    21061 aaaagaaata gtaaacagag ctaggagctt tgggaaacca gtgatagtag cgacagaaat
    21121 gctttattca atgacggaaa gtccaactcc aactagagct gaagcgactg atgtggccaa
    21181 cgcagtattt gatggaactg atgcattaat gttatctgaa gagacggcgc taggtaaaca
    21241 cccagtagag gcggtgaagg taatggacaa aattgctcga ttttgcgaaa attcaataaa
    21301 ggtaagtacc gaggtgatca aagcaagtaa tttgactgac atcctgaccg aaacagccac
    21361 gaaaatgatt tctccagaaa ttaataatcc agccagaagt atcatcgtgt ttactagagg
    21421 tgggacctca gcaatactaa tgtctagaca ccgcccacga acaccaataa ttgctataag
    21481 caataacgaa agggtcttga acagcttgtg tttatcattt ggcgtactac cgttttttaa
    21541 aaaatttgat acagaaaaat atactagtga agacccaata tttgatcaaa taaaaaaacg
    21601 tggattacta aaaagtaagg atatagtagt gataatacat ggtgatagct ggtttggact
    21661 tggaccggcc aacagattga gcattagaac actataagcc cgcaaaattt atttgtagta
    21721 gcttttgact tgaaagagga tgtacagaga aatagctgat ccgataataa gaccaccaag
    21781 attaagactg acaactgatc tgagagtatt aacaagtaca gagaaataaa ccaatctcca
    21841 agcagaagct ttacgagcaa aaagccccgg gatagccata gtactcaaga caattgaagc
    21901 gagcaaaaat agtaagtcaa gagagaaggc tgagccataa taaacgccgc caagataggc
    21961 ataacgatta ataagtatac caaggccaaa aagagttaat agggctggta cggaaaaaat
    22021 taagacgata gcagccaaat aaggtgagta cttgacgaca atgtccttgg cttcggcagg
    22081 tagggcgggg gctttcttga cagcataaag ttcgataaat gtctcaaggc gagaaagtaa
    22141 ttgcttaatg tccgttccag tctgtttgtt tgtattcttc attaagaata tttaataaca
    22201 gatggcgaca aaaggcaatc tatcagaatt gtggcttaga ggagctgttg aactcaagtc
    22261 tggcaagacg atctttgaga ccgtctcttt caagagagat ttcgaccaat tgttttttta
    22321 gatcatcaag ctcaactttg actttttgtt ggtgacgctc aaacatgatg ttgttaactc
    22381 tggccaaact atcgaaggtg cccgcgtctt cccataaacc accgaagatg ttgaccttaa
    22441 gctcttgatt ttgaagatag atattgttca aatcggtaat ttctatctct cccctagctg
    22501 atggttttaa gttcttagcg tattcaacaa cacggttatc ataaacataa agtccgacaa
    22561 ctgcgtagtt acttttagga gtagctggtt tttcttcaat acttagagct ttcatatcat
    22621 cgttaaattc gacgacacca aagcgctgtg gatcttcaac ttttttggca aaaatcatag
    22681 caccaccgtt aaagagtctg acttcctcgg aaaaatcttg gtcaaaaata ttgtcaccaa
    22741 gaatcatggt aacgctatcg ttaccgataa agttnnnnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaataaatc attggcttgt
    22921 cgtagaccgg caagagttgc ttgctagtaa ttttggttaa cggacgaagt ctagtagcgt
    22981 gtccacctgc taaaacgata cctttcataa tataattaaa gtaatatgtt ttttgtaaac
    23041 agggtattat aacacaatgt ataagaaacc tatagtattt ggtaaaagta cacgtacagc
    23101 gcaagaagcg gctgatagtt tgggctgtag tgtggcgcaa attgctaagt cactgatatt
    23161 catagatgac agtaatcttc ccgttctagt agttgcttca ggttcaaatc gggtagatga
    23221 aaaaaggttt gggctacaaa aagcagatgc tgattatgtt aaggacaaga ctggttttgt
    23281 aatcggagga gtgccaccat ggggacacaa gagtaaaatt aggacaataa ttgacgaaga
    23341 tttggcaaat tatgaggaaa tatgggcaag tgctggaaaa acaaattcag tatttaaatt
    23401 gacttttgag gagctaggaa tggagacaag gggggaaata cgagctgtaa agtgaaactc
    23461 aaataaagcc gattattttt tcttcttcca gataaacaag ttgtatattg tccaattaag
    23521 tgggacagtt agg
//
LOCUS       DDWP01000004            6718 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_27230, whole genome shotgun sequence.
ACCESSION   DDWP01000004 DDWP01000000
VERSION     DDWP01000004.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_27230"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1827 a         1463 c         1151 g         2277 t
ORIGIN      
        1 cttctaataa tcatctctgg tccgtcttgt tctggtaaat ccactttagc caagaaaatt
       61 tctgaaaagt ttcaactacc tttaattacc aaagacggca ttaaagaaac aatttttgac
      121 caccttggtt ggagcgatcg cgagtggtcc aaaaaagttg gtgctactag ctacaagctt
      181 atttcttact tccttgacac aatgttggct tctcaaaggt cactaattat cgaaagcaat
      241 tttaaacctg agttggacag ccaaccaatt cttgagaaat taactaaaca caattacttt
      301 ccactccaga taatgtgtca atgtgatgga caaattttat ttgaaagatt caagacaagg
      361 tctgaatcag gcgaacgaca cccgggccat aatgacgaca aaaattatga cgagtttaag
      421 gacatgctcc ttaaaggcaa gctcgacccg ctagatatag gcggggagat actcactctt
      481 gatactactg attttgatag tgtcaattac gattccgtat ttcaaaaaat cgtccagctg
      541 atctaattat tttttcggtg tattatattc ctatggataa aaaaatatct tcagaccata
      601 aagactgttg ttctcctaaa aagtttaaac atttgcgttg tcatcatcat ggtaatcctg
      661 atgcaattta tggtctaggt gttattggtg ctttatttta ctttcttcaa ggcgcccagg
      721 ggtttagtga aatcattaca ggtataggta aatcagtttt ctggcctgct gttttaatgt
      781 tcgaattgct cacttatctt cagctctaat ttgtttcttt cacccggact tgacttgtca
      841 cgtataatag gacaatactt ttaccattag tcgccgggac tttatatgaa aataagagat
      901 agaaacaaga tcattaaagc catctcttat agctcagcca atcgagccga aaatttttta
      961 agtttaaaat ctccagaaca aatcgaggtt attctcttac ttagtaagca cgttcagctt
     1021 cagcttttaa aagatatcac tgaccaggat ttaattcgtc ttattaacca cctcgatcca
     1081 aacgatgcca ccgacattat ccagctacta ccctcaactc gacaaaagga tatcctcagc
     1141 catcttaacg aatatttaaa atcacatgtc accaaccttt tagcttttga tcaagacact
     1201 gctgctggtt ttatgaatgt cgactatatt caggttgtcc caagagacac tgtttcatct
     1261 gtcgctaaat cagttaaaag atacgagaaa cgtaccggca aagtgccgac cattttgata
     1321 gttgatcagt cggggctttc aggtcagctg cccattcacg aactagcttt tgccaagcca
     1381 acagactatg ccatcaacta tagtaggaaa atcagcacta ttgattataa tgttaaatca
     1441 gacggggttg tcgactattt tcgtagccat ccccacagca aggttgtagt tattggtcgt
     1501 cataaaaatg ttttgggcat aatctatatt gacgatattc ttgagctact tcataccgat
     1561 gagacttcat cactttacga ttttgctggt gttagtgaag aagaatctat atacgacaat
     1621 gtcactcgca aggttaactt tcgttacaaa tggcttctta tcaacctcgc tacttctttc
     1681 ctcgctgctt tcactgtcgg tttatttgac gagataattt ccaaatatgt ccttttggct
     1741 gtctacatgc ccattgtcgc cggcatgggc ggtaattctg ccactcaaac tctggccgtc
     1801 atggtccgcg ggctatctca aggagaagtc aagtcaaaaa tattacttca aactctcaaa
     1861 aatgaaatca tgtccggtat tattaatggc gtcataaatg gtcttattgt cgtcttagtt
     1921 atctaccttc ttgatggtga tttatatgtt ggtatcgtct tggggctagc catgatcgtt
     1981 aatctattca ttgccggtac ttttggaact ttagttccag tagttatgaa aaaactcggt
     2041 aaagatcctg cttcttctgc cactatcttt attactaccg ccactgatat ttttggattt
     2101 cttacctttc ttggtctggc aactattatt cttaagtaga atctctagcc atatttttta
     2161 aataaaaaat accccctttg agatactcag ggggtatttt aagaattgat tctagaaatt
     2221 atctaccatt gtgcattccg gcaccttgtc catttctttg acccattcca tatttagctc
     2281 taaaagcatt caacaaagtt gaatcacctt tttgggcttg gttttgagcc aatgcaaatt
     2341 ctttgaagtt agattcattt acaaatctag tcactccacg accttgcatg tttgcttccc
     2401 aggttttgta atcacctttg gcaaaagcgc tcaagttggc ctgatgtctt tcttcggtat
     2461 aatttggacc tttgacacta gcatcaccac ggtaggcgag tactggagta aaagagttaa
     2521 cggctactaa tcctagagcc acagcggcta atcctaaaac gattttggtt ttcatttatt
     2581 gattgaatta ttaatcagac ttgcgcagtc tattactaat acaatctccc ctttagtttt
     2641 ctttcatcat atttcgtccg tttccactcc acggtcgcaa ctcttccgcc acaaaaacat
     2701 tttcactcat catattaccg atcaatttca cctcttgatt gattttaatt tccactcgtc
     2761 cacgaattaa tggtttaccg gttatcaccc aggttttatc ttcaaaatct tctaacaaaa
     2821 aattatcatt attaatttca actattttgc cagataaata accttgctct ggattgttcc
     2881 acgctgtcat tttcatcggc accattgtcc gatagtaagg gacacctgct tccaaccgtc
     2941 tatctattct acttggtaat cccaatttga atactacccc tcccactatc agtccaatga
     3001 cactgatcat agctatcacc tgccaacttt gaaagcgata ggctcttttt gttttctcaa
     3061 ataacacaaa tgacagtcct aagaaaatca ccactataac tagccataga tgcggtgtaa
     3121 taatattgcc tctcagccca aaccacgaca cgcttatgcc aagtattaaa aatcctaagc
     3181 taaccaaaaa cgctagccac aacccccagt ttctagccac aaactcccaa cgaggaattg
     3241 gtttaatttg ccctttgcta attaattcta gagccttatc actaatattt actttctttt
     3301 tcatagattt gtttaaattg tatttttgct cttgaaagta atgttgccac tgtccccatt
     3361 ggcttttgta atatgtcgct aatttctgaa taatctttat cttcaaaaaa ctttaatatg
     3421 actacttctt tatagcctgg ctttaattta ttaattatcg ttttaatata ttcattaaaa
     3481 tgtaacttaa tcgtttcttt ctcaatatta gtatctgcta ctagccactc cacaaactca
     3541 tcatcatttt ccacatcaat tttgaccgat tgtttattct ttttcatgaa atttatcgct
     3601 tgattatgaa ctattcgata aagccagcta ctaaacttca aattacaatc acagtcattt
     3661 aaatgtttgt aagcactaat aaacgactct tgaacgatat ctgccacatc atcatcaccc
     3721 aagtaagtaa atcggtgaac ataatttatc aacttttcct catacctttt cattaggcaa
     3781 tagaaaaaac tactatcatc tattgctagc ttgacgagtt cactatcttc ttttccttga
     3841 caactttttg gatcgaccat tatctataca ataatacaat tattaggcca tttttctttc
     3901 atttgctact attaattaat caatgttcgc aaatcatcca ggattatcta ctgccgaggc
     3961 taccaaaaga catcgtaagt ttggtccaaa tattcttcct gaaaaaccac ctcccggtaa
     4021 tatcacctta tttttatccc agctaaaaaa tcccttaatt tatgtcttat tatcagctgg
     4081 cattgtcact attattactg gccactcttc tgacgccatc attattctcc tggctgtcgt
     4141 tattaacact attcttggat tcgtccagga gaagaaggct actagtgctc ttttggccct
     4201 caaacactac gtcactagtc acgttgacgt ccttcgcgat ggtcagaggt tgtcgcttga
     4261 tgtttccaaa gtagttccag gtgacactgt catcttaaat caaggttcca agattcctgc
     4321 tgacggagtt ctcctttctg ccaaccgtct ttatctggac gaggctgtcc tcaccggtga
     4381 aagtttgccg gtcgagaaaa aggctaatga ttctgtttat atgggcacga cagtatcttc
     4441 cggccaagcc attatgtttg tcaatagtat tggggctagc accaagatgg gagctattgc
     4501 cctccaaatt caagagtcag aagagttgac tcctctccag cgccaacttt ctgttttcag
     4561 tcgacagcta gtggttatta ttgttatttt aaccattatt gtttttcttt taggtatttt
     4621 tcatggcttt agttggctcg aagttttcag cacttctgtc gccttggccg tctctagtat
     4681 ccccgaaggc ctaatcgttt ctttgacggt agtcttggct attggcatgc agaaaattct
     4741 tcgtcgtcgt ggtcttgtca aaaagctggc tgctgccgag actcttggtg gagttactgt
     4801 gatttgtgtt gacaaaactg gcactctcac tcaaggtaaa atggaagtcg ttgattatgt
     4861 gggcgacaaa aaactgctgg ccacccaagt taccttagct aatgatttag atgatccatt
     4921 agttatctct gcctttaatt ggggtaaatc tattcttaaa gacaagcttc ctaacaacct
     4981 tcgtcttgat agtattcctt tttcctccaa agaaaagttt tttattagtc ttcatcaaca
     5041 cgatcaaaaa cataatcgcg tctatgttaa cggtgccccc gatgttttat taaaatggac
     5101 aaatctatct gatgcagcta aaaagaaaat tcttgccacc atcgatcttc atacccaaaa
     5161 aggccaacgg cttattggct ttgctaccaa ggatattggc tctaataaaa agactttgga
     5221 aacctctgac gccaagtcag gcttaacctg gattggtcta attgcttttt ccgatcccgt
     5281 taggcctagc gtcaagagcg ctctagccct atctgcccgt gctggtatcc gtaccattgt
     5341 tattaccggt gactatgcca aaacatcaga gtttgtcctc tccgagctgg gaattaagct
     5401 ttcacctcaa gagatattga ccggcgacga ccttctcaat cttgactcag aacaactatc
     5461 tcaacgtgtt aagtcagtta agctctttgc taggacaagt cctgagcaga aattgatgat
     5521 tgtcgaatca ctcaagaaaa atggtgaagt tgtcgccatg atgggggatg gggttaatga
     5581 tgcaccagct cttcatcggg cagatgttgg tattgccatg ggtgaggcga ctgatgttgc
     5641 caaagaatcg gctgatcttg tcatactcga ttctgatttt tctactatca ttgctgctat
     5701 cgaggagggg agagttatgt ttgataatat caggaaaatt attctctatt taatgagtga
     5761 tgcctttgcc gaaatatttt taatcatcgg tgctatgatt cttggtttcc cacttcctat
     5821 tactgctgtt ttgattcttt ggattaacat tatttctgat ggttttcctg gactgtcgtt
     5881 gtcttttgat cctaaacgga gcaatatcat ggaagaaggt cctcgctctc ctagtgagaa
     5941 gttggttaac agttggatga ccgcgcttat tatttttgtc agtttagtcg ctgccacatt
     6001 tactctatta tcattcctct ttgtttatcg ctctactggt gatcttacta tggctcgctc
     6061 ttttgtcttt atcactcttg gattggattc cttggtttac gtattctctg ttcgcactct
     6121 tatggttcca ttctggaaaa ccaatatgtt tgaaaataaa tggttaatat tagccgtctt
     6181 tattggtctc attttccaat acttgccatt tttaactgaa ggtacacgtc aattttttgg
     6241 actaacatcc cttggggtta cttattggtt tgtagctttc tgcctgtcta tagtaatgtt
     6301 tcttattatc gaatcactta agtattttta caaaaaagta gccttttaat tttaggtgta
     6361 taatgcaatt tatgagtagc caagatcgtg ctagtgaggc ccaagcctta aatatgtcta
     6421 gactttacgg tatttctcta aaacaatcac agttagatag gtacatcctg gagagagcta
     6481 ggatggatga agctccgtct ccttatgact cagagatatt tgaacgcgtt ttaactccta
     6541 cacaaaaaag acgattttcc caagcacgtc tcaagcagcg ttctcgtcta gaggattctt
     6601 ctcctcccga attgtccatc cgtcccttcc tgatataact actacctcag tcacctcacc
     6661 acccatttgg ttatattttc ttctagcaac tactgctcca cctggttttt cttttcca
//
LOCUS       DDWP01000005           13037 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_20671, whole genome shotgun sequence.
ACCESSION   DDWP01000005 DDWP01000000
VERSION     DDWP01000005.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 13037)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13037)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13037
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_20671"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    6254..6267
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3660 a         2815 c         2283 g         4265 t
ORIGIN      
        1 atagttggtc caattctttg ttggacagtc gtagtctttt gccaattcgc accgccactc
       61 ttgaaccaac cacctcatgg ttatggaaag ttctctcctc tccttcacct tttgccacta
      121 ttggtttggc tgagtcgtgc aacaatgctg ctagcttagt aattggatcg ttagtctggc
      181 agttatttag ggtttccaag caatgctccc acacgttata gatatgatgt cctttttgag
      241 ccatatctac cccttcaaga atctctggca tgatttctcc aagtagtcca gaattcttga
      301 gtagttttat gccatctcct ggcttgtcag ttaacaatat tttaaaaagc tcatctcgga
      361 ttctttctcc agcaatttta ttaattaatt tggcattttt ctgtatcgat tcaaatgtct
      421 tctcttcgat agtaaattta atttgagaag taattctaat tgcccgcatc attcttaatg
      481 catcttcttc aaatctagtg tcaggatcac ccaccgttct tataatttta ttttttaaat
      541 cttcttgtcc attaaaatta tcaataattc gtaattcgta attcgtaatt cgtaattcca
      601 acgcaatagc gttgatcgta aagtctcttc tcgccaaatc ttctttaata gtttttcccc
      661 actcaacttt atctgggtgt cttgaatcac tatattctct ttcagttcgg taggttgtca
      721 cttcaagaat ttctttatct ggcaatacaa tactaaatgt cccataaata ttttcataaa
      781 aactattttt ggggaacaat tttttcatct cttccggcgt taaattagtg gcaaaatccc
      841 agtcttttac ttctcttcca atcattagat ctcttactgc ccctcccacc acaaaaattt
      901 cagcccccga atccgcaaac tttttcatca ctttaattac tttctccggc agtttcagtt
      961 ccattcctta ttttacagca aaaccacttt ttcttaaggt ttagccaccg gtacaatttt
     1021 tagatctctt gttactcttg ctacgaccgt tccctcgtgg aatacagtca ttccatcagc
     1081 ttgccatcca ccaccaccaa ctctaggtct gtgtagatca tttactacta cttcagtagg
     1141 tccagtttct tgatgtaatc tagcaacaat cattggatcc attaacttta aaatattttg
     1201 cgcaccttgt tccccacttt ctaacccttt cttttttttg accatattag ttaagaaata
     1261 ataaaataat atggattata tcactaaatc acaaagttat ttccgagacg atgtacctgt
     1321 tcttatcctt acttaagctc tttgggtatt cttatgccca ttttaaccat gtcgcacaac
     1381 ctgttaaaac cttgctcgtc gtccttatag ttttgtagta ttaatccagc agctcggtct
     1441 cttaaagtcc cagtcatgag tagggtagat ttgttagatt gtccaaaacg atattctttt
     1501 agtgcagtgg aagcaatcct accacctaac ttagcgattt cattgtccat agatgattat
     1561 aacatattat aggtaaattg aatcattcaa attgatattt acaccaaaaa tcccccacgt
     1621 gaagcagggg attaaggttt gatttcaaca cagattacat ctgaggtttg tcaggacgag
     1681 gtaaagcctc tttgacagca ataggacggc catcttggtc gctgccatca agagctgcca
     1741 tagcagcttg tgcttcttct tcagtttcaa actcaacaaa agcaaaacct ttgcttctgc
     1801 tagtaaactt atcaaatact atattagccg aaacaacctt accatacgca gaaaatatct
     1861 gtgtaagttg gtcatcggta atgttgaagg aaagattccc tatgaacaat ttttttgcca
     1921 tttcttacta accttttaaa taacgtgatc agacttaagg tcattcatct gatcatctga
     1981 agtataatac aaaagtgagt aaaattatca acttgagttc taaaaaattt attgaccatt
     2041 cgacttcgtc atcagatata ttgaaaattt tacttaaaaa caggaatatt aatgacattg
     2101 acgttttcct aaaaccccca tatcccacct tagagctaaa actcaagaaa gccattggtc
     2161 taattagttc ggccatccaa aacaatcaaa atattctgat ctacggtgac tatgatgtag
     2221 atggtgtcac tgccacggcc gttctctggc agactttata taaattaacc aagaatgtca
     2281 ccccttttgt ccccaaccga gagagagacg gttacggtat taaggcagaa tcattttttc
     2341 gctttcaagc cgagaaaaat attacatttg acctcttagt tactgttgat aatggtattg
     2401 ttgcccaaaa agaaattaaa aaaattcttg acaagcaaaa gctaaagatt attatcactg
     2461 atcaccactt agctaatgaa gactcaaaac tcttaactcg taactgtcaa cttatccact
     2521 ccacccaagt ctctggctca gccatatctt actttctctc aaaagaatta gacaaagatg
     2581 ctgaccttgg cctagctgct ttgggtaccg ttgccgactg tttacctctt actggagtca
     2641 accgctccat tgttgttcac gggctagagt cacttagaat caatccttct ccaggtatca
     2701 aaaagcttat ccaagtctct aatttgaaaa ataaaatttc agcctatgat cttggctttg
     2761 ttctcggacc acgtatcaac gccattggcc gtctggccga tccgaccgac gctcttcgtc
     2821 ttctttgtag tcagaatctg cttcaagctg ctaaatacgc tggagtctta aattcttaca
     2881 atcaagatcg tcaacaatta caaaaagact ctcttgataa tgccgacaaa aatattgatt
     2941 taaaaaataa actaattttt attcacggtg attataaccc tggcattatt ggtctgattg
     3001 ctggtcgcct taccgaaaaa tactatttac ctagtatcgt catttctgtt gatggtgata
     3061 ttgccaaagg tagctgtcgc agtatccccg agctgaatat tattgaggca cttcgtgaac
     3121 accaagattt atttgttgat cttggcggac atcctggtgc tgccggtttt agtattttta
     3181 ccaaaaatat ttccaaatta aaaaaatcta ttattaaatt attaaatttt aaattaggca
     3241 aattaaaatt atctccccat cttgatgttg atgctcaaat gtctcttgac gctgttacta
     3301 taaaaaatat taaagccatt gattctttgt cgccatttgg tatcggtaat ccagagcctt
     3361 tgtttttgtt taagcaagta atcattgata gtttaaggac tattggtcaa accaacgacc
     3421 atctcaagct tagaaccggt agttttgacg ccattgcttt taagaaaggg gacttgatcg
     3481 ataaattaaa agtcggcgat gttgtagacc tcgttgccag tctatcgatt aatgaatgga
     3541 acaacgcctt atctcctcag ttgattatta aagaaatagt ggtataactc caccaatgtc
     3601 agagtttttt gttgaaacag gtcaattctt taggtactct aaccaagatt ataatctcta
     3661 ttttgttgcg ggaatcagtg gtgaaccagg tgcagaaaga aaggtcaaac tagttgttgt
     3721 ggatgacgag atgtctgttt caggcgttgg tagtatctca agtggcaggg ttaatctcat
     3781 taagattggt gaagttgacg tcgacccaga actttgtaca cctatcgagc tggctgatat
     3841 gcaaaaacca agtcgagaaa aagccagagc aatacttaac aaattatgat ataactcagt
     3901 atgggtctaa gaattatggc ttacagaggt ctagaggtag ttgatgatta tgtggccgaa
     3961 ccaaaaacag ggccagcggg caatggaatt gaagccataa ccacaaaatc atttagtctt
     4021 cgacgagggc taacccccat tgatgtgagt actggtctta ctatagatcc gaaaggttca
     4081 cgcagaacag tcccgattcc cgaactgggt aacctgttgc agtgggatat acccggagca
     4141 aagactacag tctttgtgtt tagtacaagg aaagttgaca catctgggca tctttaatat
     4201 actatcccta taaagcatta gagtggccaa cacccacgag ctggaggaag aacgggaaac
     4261 ctaatagacc ctcacagaag aataactgta aaacaattca acgtgtgtcg cctttttagg
     4321 tggagcccga aaatctaaat agattattca taactccaca cgttggagtt attttttgta
     4381 aaattaatat atgcggacac taatcaaaga cactatcaaa gaagtcggtc agaccataac
     4441 tgttaagggt tgggtcaata gcattagaag tcatggccaa attgcttttg ctgatgtccg
     4501 tgatcgtagc ggtattattc aaattgttgg caacaaagaa ctagccagtc tcaaagaaga
     4561 atacgtagtc gaaataactg gtaaggttcg agctcgtgat gaaaaatatt tcaacgacaa
     4621 aataattact ggtaaggtcg aaatggaagc ccaagaaatt aaggttttat ctgagtctcg
     4681 agatcttcct ttcgacttac atcaagagga cttgaatgtt tctctgccag ttttacttga
     4741 ctttcgtgcc gcttctcttc gtaacaaaaa agtccaagat gtatttaaag ttcaggcaac
     4801 cattgtcgaa gcattccgca gttttctccg cagtcaagat tttgaagaga tatttgtccc
     4861 caccattgtt gctggctcta ctgaaggtgg ttctgaagtt ttccctgtag attactttgg
     4921 ttatcaagct tttttagctc aaagtcctca gctttacaaa caggttctta cttctatttt
     4981 cgaaagagtc tttaccgttg ctcatgctta tcgagccgaa ccatcggtca ctactcgtca
     5041 catgaccgaa tatgttggtc ttgatgctga gatgggtttt gttgatagtt ggcttgatgt
     5101 cctaaagatg gccgacgcct ctgtcaaagc aatctttaaa ttagttacag aaaaacacca
     5161 agatgtctta gatgaatatg gtgtaactat ccctaaaacc attgatgaga ctcctagaat
     5221 aaaactcaaa gatgctttag aactaatcta caagcgcact ggacgcgatg ttcgtaatga
     5281 attagacatg gatccagaag gggagagaga gatatgtcgt tggtcactag aaaagtttgg
     5341 tagcgaacta gtattcataa cccattttta caccaaaaaa cgtgcctggt actcttttgc
     5401 cgatccagaa aatcccgacg aaaccctgac cttcgacctt attggtcgtg gtgttgagtg
     5461 gattagtggt ggtcaaagaa ttcacaatta tgatgaattc cgacagaaga taatttccaa
     5521 aggtcaaaac cctgatgagt ttgaagctcc atatggtcaa acttttaaat atggcatgcc
     5581 ccctgaaggt ggttggtgta taggacttga acgtattact caaaacatgt tgggcctcag
     5641 taatgtccgc caagctagct tatttcctcg tgatatggaa cgagtcgatg gtcgtctttc
     5701 tgttattggc aaaaagattt ctaagtcaaa agttgatctt cacgaaaaac ttgtaacttt
     5761 tcttaaagag agaatggtcg atttcaagca tctcgatcat gaggagacaa agacatccga
     5821 agactcagcc agagttcgtg gtaccactct tgaccaaggg gcaaaagccc tcctaatgtt
     5881 tgctgataac aagccagtat tgctggtcat gtctgctagt aaaaagctcg ataattcctc
     5941 tttcaagaaa cagtttgaat ttaaagacat ccgtatggct accccagacg aggttgaaaa
     6001 aacatcaggt actgttattg gtggagttcc tccttttggt aatttattta atgttcctac
     6061 ttatgttgat gaaggtctat tagccaacga gtcaattgct tttaatgccg gcagccgtac
     6121 tcagtcgatt attatgagta gtagtgattt tataaaacat gccaacccgc aaaaaggtaa
     6181 ttttgccaaa taaagcagta agaaaataca aagttttttt attagcaata ccgctattgt
     6241 tgataattat cttnnnnnnn nnnnnnntat caacaccctc tgattcctga gctaactagc
     6301 aagaacatgc ccagtgtctt ggctaataat tacatcttag ttgacgttgc tactaataaa
     6361 attttgttct ccaaaggcgc tgatcaaaaa atttatcccg cttcaattac caagctggcc
     6421 acagccctca ctgctcttaa tatttatcca cttgaagagg ttatttctat tggcgctact
     6481 tactctgaag gaaaagttat ggagttacgg actggagaaa agatcaccat cagatcttta
     6541 gttacttccc ttttggtcta ttccgccaac gatgctggtt ttaatttggc caaccatcat
     6601 cgtcaaggtg tccctggctt cattaaagag atgaacggtt tggttaagca atataatctt
     6661 cggaacacta acttcactaa ctacgatggc atccattcac ccagccacta ttcaactgtc
     6721 tatgatttat ctcagcttgg ccgtttagct atcaagaatc ctgtcattac tgacgtcgtc
     6781 aaacacaaac agctcttagt taccgatata tttggtacca tcaatcaccc ccttgtttct
     6841 accaatgaac ttttaggtgt tgctccagaa attgaaggcc taaagacagg ttggactccc
     6901 gaagctggtg gttgctttat tgctcttatg aatatcaatg gacgctatct tatcagtgtt
     6961 gtcgctcaat cacccgaccg ttttgccgat actcaatcta ttctcagctg gtccaagcaa
     7021 catatccttt ggctccctta cactccctaa ttattttaga atttaggatt taaactattg
     7081 cttaatatac ttctccgtca ccgccgggac ataagcaaca aactttccgt ccccctcaaa
     7141 cacatatact ggttgtaggt aatttgttaa agttaccggt tcaaaataag ccaaatacat
     7201 ttctcttatt gtgacactac tgttgtctac atccgagact ggccaataat tacccgcttt
     7261 tagttgctca aatgcttctt cggctgtttt aattgggtaa gtcgagaaca gttctctgtc
     7321 aacgtttgca tatttgtaag tcacctcgac cacttttctt ccttccgcct gtgacccgct
     7381 caccagtacc gacaccgagg cgctattaac ttgtgaagat accactttta tatcttcagt
     7441 taattctttt ctaaagaaat ctatcttcac gatattagct tcagacaatg agctcaccga
     7501 ttttagtcca ccaaaaccaa tctcccagaa gctaactttc ctttctccct cagcaatatc
     7561 tgccggaaac tttccggctc cagccaaaaa ccctttagca atagttactg cttcttcctg
     7621 tgagggcatt ttttccggat ttagcaacaa ttggtcactt tcgtatggat acttaagtac
     7681 aaaacttcca tcaagaacgt tcattgtcag agtttggttc aaattatcat ttctgaattc
     7741 ataaacacca tacttaacct cagtcggttt attgttaaat cctaattgtt ttgctgtttt
     7801 tgtatcttgg tctagcgcca agaaggtgtt gtctggtcga actattacgt ataccttagt
     7861 ctgatctttg aactttggaa aagcgtcgtt tggtaattct gccgtaaaca gtttcttctc
     7921 aaatgttttt tctgggaaaa cagtttttgg taatactcca aacttcacat ctggcggtgt
     7981 gtacggtgga ttggctgcca agtaagcagc aataccggcc gatattccca tgtatatcaa
     8041 agtaaatccg cctataaaaa accctccata cttaattatt ctccggctaa cataggctgt
     8101 atacgttaaa gacataaatt attctacatt attttaatgc taaaataagc catgacttct
     8161 actccgatcc gtactcgtat ggctcctagt cccactggtg acttgcatat tggtagcctc
     8221 cgtacctttc tttatgctta tgctatggct cgccaaactc atggccaatt tcttcttcgc
     8281 atcgaagata ctgatcaaaa aaggcttgtt gtcggcgccg ccgatcgtct tcaaaaagtt
     8341 atgcacgatt ttgggctttc atgggatgga gaaccggttg tccaatccct tcgcataaac
     8401 atctatcaaa aatacatcaa agaacttctc gacagtggtc atgcctacta ttgtttttgc
     8461 actgaagaac gtcttgttga agttcgtaat attcaaaaag ccgccaaaaa aattcccaag
     8521 tatgatcgtc actgtttatc tttatccaaa gaagagattg atcaaaaaat aaaagatggt
     8581 gtgccctacg ttgttcgtat gcgcctcccc gatcaccaag atattgtctt taatgatttg
     8641 attcgcggcg agattaagtt taataccagt gatttagatg atcaggtgtt aatgaaatca
     8701 aacggtattc ctacttatca ctttgccgtt gtcgttgatg accatctgat ggacattact
     8761 catgttatcc gcgccgaaga atggattagc tcgaccccaa agcatatttt attatatcgt
     8821 ttctttggct gggaaccacc tcagtttgcc catcttaccg tacttctcga ccctactcat
     8881 cctggaaaaa tgagtaagcg ttttggctct gtctttgcca ggcaattttt agatgatggc
     8941 tatctacccg atgctttatt gaacttttta atgttgctgg gttggaatcc tggaacaaat
     9001 caagagttat ttactctcga cgaatttgtc aaagctttta cttttgaaca tctacataaa
     9061 aaagctgcca tcttcgatcg taagaaactt gactatttta atggccttta tattcgccaa
     9121 aaatccgttg atgaattgcg tccttatttt aagaaattct tgccacaggc aagtcctgag
     9181 caagtcgaag ggctgattcc tgttttaaaa gagaggttgg taaaattttc tgatcttcct
     9241 gagctggtga agtttttgtt tgaagatgtt gtctacgaca aagaattatt attaaagaag
     9301 gggactagcc cagagttagc cagactgatg ttagaagagt caattaaatt gttaagtgat
     9361 tttgacaatc tatctgaaag attattaaag ctagtcgaga gtcataattg ggacaaaggt
     9421 gaattcttta tggttttccg agtagctatc tgcggtgtcg cttttacccc gccggtagtc
     9481 gagtgccttc cagcactagg tcaagaaaat acaattcgta aattacgact tgctcttagt
     9541 ctgctagaat aatttgtgtt attgctaaca atagctttat tttttctatt ctcctcgcca
     9601 gtatctgctc aagataccgt gagtccaact gccacacctt ctgcagtacc aacatcggtt
     9661 gtcaatccaa gtagtggtat ttatattaat gaattcatgc cagctcccga agataatcag
     9721 gaatgggttg aaatctataa tgataacgat tatgcagtaa ctttggaaaa ctggaaattt
     9781 gacgatttgg ccgatggtgg tggtcagcct aaagagttag gaactattgt ctttggtccc
     9841 aaagagatca aaacatacga tttaggtgga ggttacttaa ataataccga tgattgggtg
     9901 aggttgttaa atcaatcaag tgaattaaaa gatagctatc attattcttc aattagtagt
     9961 actttaagtt ggtcaaaagt ctctggttca tggtgtctta ccactccttc aaaaggtagt
    10021 gtaaattcac catgtccatc tcccacaact actccaatca ctcccacccc tacctcaact
    10081 cttactccag ttcctacagc caccaagact cccactccca ctcctacaat tactccaacc
    10141 acccgtccta ccgctactcc tatccccaca atcgctatca ttcctaccga gccacccctc
    10201 actccagtta ttgaacctac ttcaagcctt ttgtctctcg agccttcacc caccatcagt
    10261 gaagatatac ttggtatcag tgatattatc aaaatctctc ctacatcttc tccctcaaaa
    10321 agctttaacg gcataccttc aaatttaatt gccataattt ttattgctat cggcggcctt
    10381 cttctcttag ctccattagt aattactcga gtcagaaaat gaaatatata cgttttctcc
    10441 cagctattat ttggatgtta tttatttttt atctatcatc agtgcccact tctggtatcg
    10501 gcccgtcagg cttttggaga tttatttttt ttaaatctct ccacctaatc gagtatgcca
    10561 ttttgttttt attactccgt tttgctggaa tttctaataa atactcacta cttgtctcct
    10621 atatttatgc tgtttcggat gaagctcacc aatactttgt tgtgggtcgt tcgggcatgt
    10681 ttcgtgacac cttatttgat tttaccggtg catttttggc ttatattttt ttattgttga
    10741 catcagtcag aagcaagtct aagattattt aaattatttg tttttgtttt tatgaaaaaa
    10801 attactctca gtattttggc aatcgccatg accttagctg ttgttaccac ttcagcttcc
    10861 gctttattta gttctactgc tactgtctcc ggtgtcactt tctctaccgg taatgctaat
    10921 cttcaagttt ggaatggttc tacttgggtc gctgactaca atccaggaaa cttcttattc
    10981 cagaatatgt ttccttccta tgccggttcc caaacttttt atttgaagaa caatagtacc
    11041 tcggctatta ctctcggaat taatgccaag attcgtaatg gtgccactga agatccatct
    11101 ggttcttgga atgtactaaa agatgttgtt gaggttaggt tttatgatat tgaaatggca
    11161 acaccagcca gtggttgggc ctcacttaat tattggaata cgactggtgc caactttgtt
    11221 actgctggaa tttccaaagg tgcacaacgt caatatcgag ttgatgtccg agttgttggt
    11281 tctgctggta acgaaatctc tgacaaaagt cttactaaca tgaagtttga cttcactggt
    11341 actcagcaat aattttaatt ctctacagaa caccgaatgt attttcggtg ttctagtagg
    11401 tgattaaaaa aataattgaa aatactgttt ttgcttttct tcttcttgtt ctagctattt
    11461 ctatatctcc ggttcttcct tttaaaaatg ttcctcgcac ctatactgtc gtttctggtt
    11521 cgatggagcc aacaatcaag actggttcaa ttgttttaac taccccagtt gacccaacta
    11581 ccataatggc cggcgacatc gtcgccttta cctctccttc aaattccaaa gacactattc
    11641 ttcatcgaat tgtcgatatt aaatctactt ctcctctgcg tttccagaca aaaggtgaca
    11701 ataataatac tgccgatgct tgggacctaa tggacgtcgg cattcttggc aaacatgttt
    11761 ttgccattcc ttatattggc cacatcgctg cttttattcg cacccccatt ggttttggtg
    11821 ttctaatcgt tatccctgct ttgcttttta tcatctccca aattctaaat atcaagaaat
    11881 atattaaaca agaaattgac cgacaagtaa aaagcaaaac tgtaccacta ttctttatcc
    11941 tatcttttct aatatcctct tcaatgctct ttacaggttc aatttttgca aatttctata
    12001 gtaccactag tatttctcag gtcacttttt ccactaaaga ttttgtcgct ccaccaactc
    12061 ctcagcttat tgcccctact aataattctt atcgtaatac cgtcgggcta gttatggact
    12121 ggtccgatgt tggtgattat caaaatcaac acaatcctgt ctactacatt taccaatcct
    12181 cacgaaatct ttctttttct cctttagctt atcaatcagg tagattaact tcatctcaaa
    12241 ttcctgctcc gggtactccc gctggtgttt actattggcg agttaaggct tgcgattcag
    12301 ttgataactg ttctgcttgg acttctcctt ggaaagtcac cgtcgacaat cagtcgcccc
    12361 aggttgtcat taacgccccc attacttcac cgacttctgg tttagtccct atttacatta
    12421 cctccattga taccaaccct catcattttt ggttagttgt agaaaactca tcaggtgtca
    12481 acgttgctgg tccaggaact actactcagt catctactct cataaaccac ctagctttca
    12541 attgggatac aactctactt cctgatggcc tttacaccat caagcttgaa gcacgtgatg
    12601 ccgctggtaa taaaacacca aacttgtctc cagtgccttc tgaccctgaa gttgtcggag
    12661 actcagtcga ctggattaca gtctatgttg gtaacgatac ccaatctcct agatccacta
    12721 tcaccgaagg tcaggcagat acccaagaga ctcatacctt tgatatttcc tattcttcat
    12781 ccgacttaga tatcgattat gtgaagcttt gctactcata taattttgga actgatattt
    12841 gtggagacac caacaactct ccgtccggtt tgtttacttt cgattctcct tttggtgatg
    12901 gtctttatag tttttatact atcgcttatg acctagctgg aaatattgaa gatcttaagt
    12961 tttatgaact tgatgttcaa gtagatacaa aagttccaac caccaatgtt gatttaaagt
    13021 ctgtaaccac tagtcac
//
LOCUS       DDWP01000006            3773 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_39923, whole genome shotgun sequence.
ACCESSION   DDWP01000006 DDWP01000000
VERSION     DDWP01000006.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_39923"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1094 a          764 c          619 g         1296 t
ORIGIN      
        1 gttgtataat ctgctgaatc tttttgagaa attgccaaat caatgccagt tccaattagt
       61 ttgaaatcct ttttatctct ctcgatttca ttgtagaatt ttatccattc agggtgaacc
      121 aacctatcag catcagaaat tatttttaac aaatattctc gataccatga agattcagac
      181 ccaatgctag atctcaactt attaattgct tccatgtttg ggaactttcc tggccacgcg
      241 atcgtttcat gtttatcgac tagtggaaca gatataaatt taccgctaag ttttttatta
      301 tctatcatcg ctttgagtcg catgagtaaa caatcctcat ggagtaagtt tccgacgata
      361 atatattttg taaaattatc accgaccggg aacacctcac cagtcagcca ttgagaagtt
      421 ttgtctcgac tctccctagt tttagtattg tttaagtcct ctacatcgtc gcagatcact
      481 aaatctggtc gatattgacc gtgtcttaaa ccacgaatac tctgttccgt tgaggcaacc
      541 gctattctgg catcaaaatt agataaaaca attgatgtcg acccccattc gtcattaact
      601 tcctgaaacg gcccgatatc ctttttaagt aattcgttag tttccaactc tctcttaata
      661 ttagtgagat gaagcctggc ctgttgttga gtttgaccta caattagtac aaacttcttc
      721 tgttgacttc cagtaattgc ccagattgga tatgacaaac tcattattgt cgatttgctt
      781 gaccccctaa aggcgatgac aattcctaat tttaaatttg tatcttcggt cagggaaaac
      841 atttcattat gaaacggagc aaatggatag ccaatgtagt ggctaaaata aatagtaaaa
      901 aaccagaagt ggctgtttct ggccaacgcc ttgcggacac ttcttttctt ttttatctcg
      961 acgagttcac tttcatttaa tgtttggatc atctttccct ccttcaattg aattttctaa
     1021 cgtgtttggt aagaccgagg ccaatttcag tgccttcatt actagtgctt cctgctctgg
     1081 cgacaattgc tcatcgtcgt gttttaattt ggcagttact tctactctcg tggcatatga
     1141 tggatgatgg ttacgtaacc aaaaaataat ggcggtcaaa ttcttatctc taattgcact
     1201 cataagttga gattcagcca tatcattaac caaaccatta ccatcagaaa ttgcttcgtt
     1261 aactgcggcc gcaaactttt catcatcgct tttccaacga taaaaactgg ctcgaccaat
     1321 gccgactttt tcacaggcca cttgaaccac aggtgtcttt tttagttgtt caattaacag
     1381 ttgtttttca ttttttgttt tcatactttt tcagtttgtt aaatcgatca ataattgttt
     1441 ggcagtactc aggatcaatt tccaccatca agcattttcg ttttgtttgc tcacaggcga
     1501 taagagtaga tcctgaacca ccaaaagggt cgtaaacata atcaccaatc tgagtactat
     1561 tgaaaatcaa atgtcgcaga agtgatattg gtttcatggt tggatgtagt gggcttttgt
     1621 tgggtttggg aaaagccaaa atacttttat ccttactctt aaagaattgg tgggtgccaa
     1681 accagccata agcgatcaac tcgtgttggg gtagataatc catccgacca atgactgagt
     1741 ggttttttat ccagattaaa agttgacaaa atttgaaacc ggaaatatca agcgcctttt
     1801 taagcgcaaa caacattttg tcggagttaa aaatgtagta actattttta gtggataaat
     1861 gaggtttgat gacctctaac cattgttgag taaataattg atacgactca tctgtttgaa
     1921 cccgatcgtt ggcaattact tttctcttaa tttgcgattc tgagaacccg tttttacttt
     1981 ccacgtacgc tacaccataa gggacatctg ccaaaataag tcgaatactt ttatttgaaa
     2041 gtaacttttc aaccattacc ttatcagtcg agtcaccgca taaaagcagg tgatcaccaa
     2101 gaataattat gtcgccgtta ttaattgaca tacttaacct ccttacccgt aagtttttgg
     2161 taacgggaaa taattaaatc gcaaaatatc ggctcgtatt cgatcatcaa acatttccgt
     2221 tttaattgct cacaggcaat taatgttgaa ccagatccac caaacaaatc taaaacggta
     2281 tccccgacgc tggtgcaacg ccttagcgcc ttttcatgaa gggtagggct cttttctgta
     2341 ggatgttcat aatctccacc cgacaatctt ttcaccaacc aaatatttag caaatccaaa
     2401 atgtcgtcta tagttcgatt acccgagcca atctctttat tgagaatttc gtttaagttc
     2461 tttgggtttt ctgaaattgt aggcttgccg atagttccat aaatacaggc ctcgtacgat
     2521 ttattaaaag caacatttgg agtaggcgaa aagccatttt taatccacaa acaagtcctt
     2581 ttattttcga ttcccaattc tgcaaataat ccctgaacta ggccaatgtg gttttcatcg
     2641 ttccaataaa atatatgaca atctggtttg gagatagtta acgcattttg gattgtcttt
     2701 ttcaaaaact ctttgtactc agagtcagtt ttgttgtcat cggtcttacc gccatattta
     2761 cctttacctc cgactccttt gttgtagtcg agagaaatat tgtagggcgg gtcacagtag
     2821 accattgata ttgtcttttc tttagttagc ttttgaacaa cctcaaggct tgtactatca
     2881 ccacaaatta atagatgaga accgagttga ataatatctc caacttgtgt tttaggaatc
     2941 ttaattttct ccaactcttt ttcggtgtcg aaattatcgt cctcaatatc gagattttca
     3001 tcccaaattt tggacaaatc attattatca aaaccaatat ccattaacat ttcaatatca
     3061 aaattcttga gtagatcaaa atcccaatcg ccagacacct tatttaaagt aaggttaagt
     3121 tttttctctt tttcaatatt atcaatgtca ataaaattta cagggacagt tttgtaccct
     3181 aagtctttgg ctatttttag cctaaaatgt ccagcaataa caactccata acgagattta
     3241 ttgccattag ccaatattgg caccacaaat ccattttctt taatactttt cttcaaatca
     3301 gagatggctt tttcactcca gtatcgaggg ttgtactcgg acgggattaa gtcattaata
     3361 tttacctcaa ctgtttgaat tttcgtttta tttttcatat taattaaatt ttattggtgt
     3421 ccaactaaaa acttatatat ttttagttga gaccaaatta atatgggaat aatattcgca
     3481 aacaaaaacc aaaaataccg tgtcctttaa gcttgaaggc gacctaatct ttttttatga
     3541 cgttttaccg attcactagc ttctttgtag gtataaaggt ctaaatcaaa ttcttcagcg
     3601 atcatatcgg caatttttga cattttgtat cctagatttc taagctcaga tgctctttta
     3661 tcaacatcca acatgggagc gggttgaaac tttttagatt tgtcgggtaa tcttttctgt
     3721 tcaaccatca ccatcggcca cataaacttt atatcctcca aagtagtatc tgc
//
LOCUS       DDWP01000007            2793 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_20924, whole genome shotgun sequence.
ACCESSION   DDWP01000007 DDWP01000000
VERSION     DDWP01000007.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_20924"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          800 a          569 c          543 g          881 t
ORIGIN      
        1 tcaagcttga attgaattct ttgccccgat cacgaaaagt cactcggaaa atgctaatat
       61 catttataac gctaattaat ttatttcatc ttctgggatc gtccaccagc ttatttttcg
      121 gacctattat ttactatgtt aaataaaaat tctcaaagac ctccggttcg tccgcaggca
      181 aacttttctg gtattaaatc accagttcgt caatttaata gttctcaaga ttccgtaagg
      241 gtagtttccc ttggaggttt tggagatgtt actcaaaaca tgttcgttta cgaatatgtt
      301 cccaatggtg atctcagcaa aagtcaacta atcattgttg actgtggagt cggttttcct
      361 gaagaagatg cttttggtgt cgaccttcaa attcctgaca ccaaatactt agaagataaa
      421 aaagatcgaa ttctcggtat tttcatcact cacggtcatg aagatcatat cggagctatt
      481 cgctttatct tgcctgttct tggtcaagat attcctattc atgcacctcg tatggcagct
      541 gcttttattg agaaccgtct ggttgaggct aatatccgtt ccaagatgac tatctatgag
      601 gccggtgaca ctatgacagt cggacctttt tctatcgagg ctatccgtgt taaccattct
      661 gtccccgaca ctaatcactt tgccattact actccaattg gagtctttta tcatggttca
      721 gattttaaat ttgacctttt ccctctagat ggtaaaccta ccgattatgg tcgtattgct
      781 cgcgcaggtg agaagaaagt cattgcctta atgtcagact gtcttggtgc tgatcatcca
      841 ggattctctc cctctgaagg ttctattgct gaagattttg agcgcaatat taaaacggcc
      901 aaaggtcgtg tttttgttac cgccatttct agtaatgttg tccgttgggg gcaggctatc
      961 gactacgcca agcgtgccgg tcgcaagatc gttaccgtcg gttattctgt cgataaagcc
     1021 attaaaattg gccaagcctt aggctacgtc aatctaaccg atgctgacat tgtcccacca
     1081 gagagagtca aaaactttgc ggataacaaa cttattttcc ttgttgccgg attcgctgct
     1141 caagctaatt ctgcactctc gaagatggtt atgggcaaac atcgtattaa gataaagtct
     1201 ggtgataaag ttattttctc cgcccctgac tatattcctg gtactacctc caagatcttc
     1261 gacttaatcg atatcttgtc taagatggga gcagaagtga tctccggaga tggacaagag
     1321 atccatgttt ctggtcacgg ttttcaaaaa gagcatgctc ttttgattaa tttagttcgt
     1381 ccaaaattcc tattacctat cggtggaaat tatcgtcacg tcaagagtta tttcaccatg
     1441 gccaagacca tggggcttaa agatgatcag tttatcaacc ctgattctga ctcaattgtc
     1501 actttttatg ccaacggtac ttacgacacc aaataccaca accctatccg taaagttctt
     1561 atcgatggct taggtattgg ggatgttggt accactattc ttcgtgatcg taaaatactt
     1621 gctgaagagg gaatgttctc tgttgttctt atggtcaaca aagattctgg tgctatgacc
     1681 cgaccaccgg agatactttc tcgcggcttt gttttcttta aagagaatgc tgatctgctt
     1741 gagtatctaa accaagaaat aattcttaag ttcaaccaag ttaccagtaa gcctgctaac
     1801 tttgaatata ttcgtcaaca agtacaagca catattgagc agataatcat ggacaaaacc
     1861 ggccgccagc caatggttct acctttgatc attgaagttt aaaaatatac gtcattgcga
     1921 gccgaaggcg tggcaatctt tactaacatg acattgtgtg ttccgaagac agtcattggt
     1981 aaattggaca attcaattcc atgtagagac gcacgatcgt gcgtctctat aagataatcg
     2041 aagaatcgcg ttaccaataa ttatcaaatc caccacacat ttgtttttct gatattatat
     2101 tttcaagccc gtgttgtgta taaaatttat tttgtgcacc gcgggctttc tagttttttt
     2161 aggcgaaaat ttgaacgtag tgaaaaattt ttcctaaaaa cacggtccag cgagcgtagc
     2221 gagtcggata tatacccctc tggacaattt aatcattttt ttgtataaac agtcccatat
     2281 gaatcaagaa caaattaaag aggcaaaact acagcttgcc aactatattc gtgctgagag
     2341 aagatatcga atttttctgc atcatgttgg tgttggtggt tcaatactta aagcatcaga
     2401 acaaagattg tcgttgcgtt gttatgacta caacaagttt tgtcttgaaa atcctgatcg
     2461 cggtttggat caaactgagg tataataacc tcaaaccaat gaagtggttt cccgccacag
     2521 aggtgactac gaaataagta gctccggaat tgaaccgtat aaattcaaaa gagacaaagt
     2581 taaggtggta ccatcctgag ttttaggatc cttaaattaa atattttaag gacacctatg
     2641 gcaacgtacg atgacttcat taaactcgat gttcgagttg gcaaagtaat tttagttgaa
     2701 gattttccag aagctaaaaa acctgcctat aaactaacta tcgattttgg ttcagaaatt
     2761 ggcattaaac attccagtgt tcaaatcgta aag
//
LOCUS       DDWP01000008            6091 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_24188, whole genome shotgun sequence.
ACCESSION   DDWP01000008 DDWP01000000
VERSION     DDWP01000008.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_24188"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1921 a         1355 c         1039 g         1776 t
ORIGIN      
        1 gcgggtccta tgggattcga acccacgatc ttccgcgtga caggcggatg tgctagaccg
       61 ctgcaccaag gaccctagtt atcaacggct ttgataatat atcaaaaatt tatactttta
      121 tctacaagat tgataaatac ttaattacca ttaatctgct tttccaaaaa catatttacc
      181 aactttttga ataaattctt attatttaga tactggtcaa agataatatg gtcaatacct
      241 gcattttgag caaactgaga atcaacttct gaatcaccaa agtaaataaa ctccttagaa
      301 tcaagccctg atctattcat caactccaaa agtgaatacg gatttggttt cttaaaacca
      361 gcatcgatac aagagacaac ttcatcaaaa taaccataaa tattatttgc ttcaagtacc
      421 ttattcatca aatctttttt tctattagta caaatataaa gcttttttcc actagacttc
      481 aaaaactcca acatctccac tactccttca aatagttgca gtctattaat attcttttgc
      541 tgaattacat tatcttgttt taacaactca ggcacatctg cctttaagtc aatttctttt
      601 aaaatatgtt tatataaatc ttcaatagtt tgacctttaa tattgtcatg cagctgacaa
      661 acgagatcaa aactaactga tggatcgtga cttgttatca ctcttgcgat agtctctccg
      721 caaaattgtc gtgcgtccac caaggtatca tccaaatcaa acacaaacgc atttttgaag
      781 tttgtatttt tactagtact attagtacta tctttcacat tttcaaaaaa tgccgtcacc
      841 ggcttcttta acaccttaga tatcttaatt aactgaggaa tcgatatctg actaacacca
      901 agttcatacc tacttaccat tatttctgta cacccaagat gtttagccat ctctttttga
      961 gtcaccccaa tctccgacct aaactccttt agcttctttc cgatttcatt ctccataagc
     1021 acggattata catcagagta ctagttacaa tcaagcatat ataaatgtta taatttatag
     1081 attagtaatt aaccttacaa aaatgattat tgctctagcc atcgctgcca taatagcttt
     1141 atattttatt actttttata acggcttaaa gacaaccctc gttcatatca ccgcgtctat
     1201 tcaagaaatc ggtaaccaac taaaacgtca atcaaatctt atccctaacc tcgtggaatc
     1261 cgttaaagga tatatgaaac acgaaaaagg aatctttgac gaattaactg ctgccagaaa
     1321 attaatcgat aacgccaaag caggtgattc cagaagtgtc gctgcggctg aggactcgat
     1381 caacaatgtt ttgaaatctc ttcatgtcat tgttgaatct aatcctgaga tcaaagcttc
     1441 aactgttgtc tccgacatga tgcaagaact ccgagatact gccgacaaaa ttatgtatgc
     1501 tcgtcgtaca ctaattgatt taactgccga ctttaacatc aaaatatcta ctgttcccgg
     1561 tctgtggatc gccccaatca tgggattcac tcctcaaaaa ggttttgaca ctccaacttc
     1621 cggagaacac ctagaactat ctgaaagcga ggcctctaat ccaaaagtag atctaaacta
     1681 gttttacttc ccccttgtca aagggggatt gagggggatt gactatgctc aacatctacg
     1741 aacaaataaa tcataacaaa tggcaatcag tactaattat tagtattttt atcggcttcg
     1801 ttgtatttgc tgcctacgcc attggctatg cctttaatat tcgagaactc cttcccttag
     1861 ctataatctt ttctattttc tcctccggtg ctagctattt ttggggagac aagatagtcc
     1921 ttagtcttaa cggtgctcgc cctgcatcta gaaaagaatt ctttgatttt tatactgtca
     1981 ctgaaaactt atctcaagtc gccaagatcc ctgtccccaa aatctttgtc atcgattcac
     2041 ccgccatgaa tgcttttgct actggccgcg atccaaaaaa ctccgtcgtc tgtgccacta
     2101 ccggattact ttccaaacta aaccgtactg aacttgaagg ggttgttgcc cacgagttgg
     2161 cccatatcaa aaactacgac attagactta tgactatagt gtccatatta gtaggtacac
     2221 tttctatctt aattaatatg tcttggcatg cccgaagtgg taataacgac aataagggta
     2281 acaacgcttt aatgatagtc ggaataatat taattatctt tgccccatta atagcccagt
     2341 taattcaact agctatctcc agacgtcgtg aatattttgc tgattcttct agtgccatgc
     2401 ttaccaggca accaagcggc cttatatccg ctctaaaaaa acttgacgct gatagcaccc
     2461 ctcaagagtt cgcctctact gctaccgctt ctctatatat cagcaacccc ttcaaaggca
     2521 acaaacttgc ttcaatgttc tctactcacc ctccagtcgc cgaccgcatc aaagccctcg
     2581 aaggagcaat gtaataaccg cattttgtta gtacatttta atttgatcta cttcataaga
     2641 ctttagactt ttgacttttg actttatact attcatatgt cttttgttca cctacatgtt
     2701 cataccgaat actcgcttct tgacggacta accaaaatca aaaaattagt cggtacggtc
     2761 aaagaaatgg gtatgaatgc tgtcgccatc accgatcatg gcaacatgta tggtgcggtt
     2821 gaattctata aagcttgtat caaagccgaa ataaaaccaa tcatcggctg tgaagtctac
     2881 atgacccaag gtgaccgcaa agacaaaact tcccaaaata aaaaagtaaa tcatctaatt
     2941 cttcttgcta aaaacaatac tggctataaa aatttaatga aattggtcag cctcggccat
     3001 cttgaaggct tttactacaa accccgtatc gattgggaac tcctcgagca atatcatgaa
     3061 ggtctaatct gtactagcgc ctgtcttgcc ggtattgttc cttcccttat taatgacaat
     3121 caatacgaaa aagctctaga gaatgccacc agatttcaaa aactttttgg cgaagactat
     3181 tacattgaaa tccaacgaca cccaggtctt aaagaaatcg acactgccaa tgagggtctc
     3241 atcaaaatca gccgagaact aggtatcccc cttgtggcca ccaacgattc tcactacatc
     3301 aaaaaagaag atgcctcagc tcaagacgtg ctagtaatga tcaataccca gaccactgtc
     3361 aattctagta accgcatgag tatggccgac attcccgact tttatttaaa atctcctgaa
     3421 gaaatggccg ctcagtttgc cgacattccc gatgctttag aaaatactca aaaaatagct
     3481 gacaagtgta acgttgagat tgaaattggc aaatggttct ttcccaagtt taaactacct
     3541 gatggagtta ccccagaaga tagactcaga gagattacct acaaacaggc agaagagcat
     3601 tacggcacca taagccccga agtcaaagaa cgacttgatt ttgaattaga tattatctgc
     3661 cctaaaggat ttgctggcta ctttttaatc atgagagatt tcattcagtg gtgcgaagaa
     3721 aacaatactg ccaccaacac caggggatcg gccgggggat cactggtctc ttttgtcctt
     3781 ggtatcgtct ctgtagatcc tttgaattat caactacctt ttgagcgttt tttgaattgt
     3841 gatcgccctt cacctccaga tattgacctt gatatcgccg acgacaaacg ccaagccatg
     3901 ctttatcaca tcagcgaaca atacggcaaa gaagccatgg ctcagatttg tacctttggt
     3961 cgaatgatgg cccgtggtgc tgttcgtgac actgcccgag ttctaggtta tgaatatgcc
     4021 atcggcgacc gtgtttctaa gatcattcct atgggtaaac aaggttttcc catgtccatc
     4081 gacaaagctc ttgaaattac tcccgaattg aaacaactct acgacaccga tcctgatact
     4141 aaaaaaatcc ttgacatggc ccgccaaatt gaaggttgtg cccgccacat atcagttcat
     4201 gcctgtgcca ttgtcatttc cccaactact gtcaacgaat ttacccccac tcaaattgaa
     4261 agtggtggcg ataaaatcat tacccaatat gaaatgcacg ccgccgaaga tgttggctta
     4321 attaaatacg atatcctagg tattcgcaat ctctcattct tgggtcaatc aattttaaat
     4381 gttcgtaaaa ctagaggtat cgagattaac cttcgtaaat taccactgga cgacaaaaaa
     4441 acttttgaaa tgttatcaaa aggcgatacc atgggtgttt tccagctagg tagtgacggt
     4501 atgactaaat ggctcaagga gttgaaacct agtcgagttg aagacttgat ggtcatggtc
     4561 gctctttatc gtcctggacc aatggacaat atttctgctt atattgattg taaatttggc
     4621 cgtaaaaaac cagtctacct ccatcctaag atggaagagt tcctaggtaa aacttatgga
     4681 gtcttggttt accaggatga tattttattt accgccatcg aaattgccgg atacaactgg
     4741 acaactgtcg acaaacttag aaaagctatt ggtaaaaaaa taccagaaga aatggctctc
     4801 cagcatgaaa tctttgttaa tggttgtgtc gaacattcag gaatgaccaa ggaaggtgcc
     4861 gaagagattt gggaactttt cgtcccattc caaggttacg gttttaacaa agcccacgcc
     4921 gcttcttacg gaatgctctc ctatcgtact ggatatatga aagccaacta tcccgtagag
     4981 tacatgtgtg ccttactcac tgcggaggct gacgatacta ctaaggttag tgatggtatc
     5041 gaagagtgcc gacacttagg cattatcgtc tctgctcctg acatcaatca atccaataaa
     5101 ggttttgata tggttgagaa tgataaatca ctcgaccaca tggctatccg agtcggtttt
     5161 gatgctatta aaaatgttgg cgatgccgct attgagaaca ttcttcacga aagagaagaa
     5221 aatggacctt tccatagttt cactgacttt tgtctccgag ttcacggtca gaaagttaac
     5281 aaacgagtac tcgaaagtct agttaaagtc ggagcttttg atcaatttgg tgaaagaaat
     5341 gccattctgg ctgccattga tgatattcgt agtaagagcg agaagataaa tagtaaaaaa
     5401 agtagtggac aaggaggact ctttgactca tcagactcag aacaagaata tattgcccca
     5461 gttgatactt tgccccaagt cgaaccaatg cctgaaaaag aaaaattagc tcaagaaaaa
     5521 ttgcttttag gtatttacgt taccgagaac ccaatttcta ccatgctcaa agatttccaa
     5581 aatgcttctc taccccaaat agaaagtatc tttggtaaaa caaccaacac tccagtaaag
     5641 tttgtcgcta ttatcagtaa gataaaaatg attcgtacta agaaaagtaa cgcccagatg
     5701 gctttcgtaa cccttgaaga tagtagtggt caaatagaag gagttatttt ccccaaaact
     5761 tatgaaacat acattaatct attaaccgaa aataaggctc tatatataga aggaaaagtt
     5821 tcaatccgag aagaagaaac gtctgtcatg atcgacctag ttagcgataa attatctagc
     5881 actaccaaaa cctacgattt tgttatcgaa gtccccaaaa acacttccca atctcaactt
     5941 atggacctaa acaatcttct taagaaaaac cccaacggac ataagggttt aattgtccta
     6001 cccaacggca aaaacataac catcccatat ggcgtaaatt acaacgccga actccaagaa
     6061 caaatcgaca ctatcctaac tttaaattaa t
//
LOCUS       DDWP01000009           24594 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_21505, whole genome shotgun sequence.
ACCESSION   DDWP01000009 DDWP01000000
VERSION     DDWP01000009.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 24594)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24594)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24594
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_21505"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         8032 a         3527 c         5946 g         7089 t
ORIGIN      
        1 gtagataaag gacaaaagat aaactgtaaa aagtaaaaag taaaaagtaa aaagtgagag
       61 atgggttttg ataaatttgg tgatgaaata tatacccatg agaagtagat aagagggaca
      121 gacaaagaca tggctctagt ggattggact gagtctcgag ttagggcggc aggaattggg
      181 gcaaagatta accagaggag cattaggttt gaaagacggt cttttttgga agatgataaa
      241 tagaagaaaa aacctagagg aagtaatacc aagctgggat ataaaagcat gccaacataa
      301 ggagccgagt gtcgggggtt ttgccagtca ccttcaaaga aaagataacg aggagaaaag
      361 tgattgaaat agcgagacag aaaatttcga gtaaagaaaa atggttgaga atagaaggtt
      421 tgatagtcta aattatttga ttcggagatg atcatttctg tctcgacttg gcttctaggg
      481 taagacagaa gactaacgac tttgagacgg ttggcatctt ggccaaaaaa taatccgtag
      541 gcaacgggta ggctaaagat aaagagggtc aagatgaatt taagtaagaa agctttaagg
      601 ttgacttgaa tttgggaacg attggtaata attagagaga gtatgagaaa taggctgatc
      661 attttggctg actggtaagt gaagagagtc agaccaaaaa ctagagcaga gaagaagaac
      721 tttttggata agaaaaaata tgaggccaga attagttcga aggttaggat gtttgtctcc
      781 caggcattac gagagaaatg gatactaaaa gggctgatgg cgagaattat agagctgtag
      841 aaggctagag ctctagactt tgaattaaat ttggtgatta ggagatagag aagaactggg
      901 gtgagagatc caaaaatgat tgaaggaaga cgaacggata tttcattaag tccaaataag
      961 tagacgaaag gtacggttaa ataagtatag aggcctggtt taaagtcgcc aaatgattta
     1021 aagattaggg gtagaaattg accaaactca tctttaccag tttgaagaat tgagtaagca
     1081 ttgtagccaa gagcagcttc gtcccagaga agtggcggag tattggatac agaatagaga
     1141 cggataaata aagaaaatac aaagatggct acaagagtct ttttcatttt agattggtcg
     1201 taatatcgaa tatcgattgc ccttgagggg aagagatatt atagattgat ttaaacatag
     1261 tttggtcgta attgctgggt gaagtgatga gtagtatttt tttatcagat ggcgggacaa
     1321 aatcttttat ttcccaatca ttgataaaga tatatttgtc aaagccgtcg acccaatacc
     1381 aatcagaata ataggcccat tttttattgg gatcgttatg atatttttga ggtgagtatt
     1441 gactaaagaa gagaataaat tcgtgaggct ccccatatct cttggtgata ataatttggt
     1501 cgtagaggtg agaattttgt tgaatatagt cggtcatttg agaatgaccg tattgccagc
     1561 tgagagcata attttttgca tagataggat atttgttcca gaaagaaaat agggtgaaga
     1621 taaccgccag actggtatag gtaataatag ccaggttgtt ctttagataa tttaacccaa
     1681 aaagaatcat cagggtgact agaggcagga aggttataga gcgaagaatc tgaggggcat
     1741 cccgggtgat ggctgagggt agtggagcaa caagagtgaa aaatagaatg aggaatatgg
     1801 ggaaggttgg ttttgttaat aacttacgac ataaagaaaa gaatcccaga aaaaagaatg
     1861 gtaaaagaag tgttgaaata agtgggaaat tggggatatt aaattgatag tgactccccc
     1921 cttggaagaa gacatagcta ggtgagaaat gtttgagata atttatagta gcgtgataga
     1981 taaaataggt atatttgttg acgatgattc gtggataatc ttgacgtagt tcgttgattt
     2041 ggttgatggc accttggtcg agtatggtga cccatttgta acgagcttgt ccggaggtat
     2101 ctaaactttg aaaagcaaat attagaagcg aaagaataaa gaaagaatat ttataccaat
     2161 ctttttttaa ggtattagat ttgacaatta aatatataag cagggcaata taaggaaaca
     2221 aaattatacg actgctgttg taggtaaaaa gacaaagtcc atagaaggtg gcactttgcc
     2281 aatatttttt ttgtagtaaa aaataaaaag aaagcaggaa aagagttaga aagaggttag
     2341 cctcaagagc gatgcgagag agaaaaatag accaaggaga gagagcgtaa atggtcaggc
     2401 caaggagagg gatgatgttt gatttggtaa ataacttgcg ggatataagg taaaaaacga
     2461 atgaggctaa tactcctgaa atgactgaga gaagtctaat tgaaaaagta tttagtccaa
     2521 atagctgaat aaatggtgta gataagtaga cgtaaagagg tagcttgaag tcgccatagg
     2581 ctcgaaagat gagaggtaaa gaattggccc attcgtcttg accagttttt gaaattgaat
     2641 aagcattgta tccaagtgag acttcgtccc agtttggact ggagggcatt tgatctaggt
     2701 tatataaacg cagaaatgca ccgaagatga gaatggtaat aataattagt ttatatttca
     2761 tctggagtta ggtagagata aacggctggt tctgatttgg tgaaataggg gtagtagata
     2821 atatgagtgg gagtggtgtc tagaaattca tttggtttgc cgataataag ggtgcgaggt
     2881 gttttaagat ctgaaggaag agatatttct ttaaaggtaa cattgttcaa attgtattct
     2941 ccgagttgat caaggaaagc aagacctttt gtctgaaatt ctgaccaacg gattgattct
     3001 tgttgggtaa tatggggatc gacggactga aagaaaagat agtaagcttg tggctcagag
     3061 agctttctgg agtaaatgat tttgtcggga ttgagagaag taatatattg atttatttgt
     3121 tggtgtccat aaagcatgcc gtgggcaaga acttgattgg ctgagtggaa ataagtaagt
     3181 gaaaaaaaga ggccttcggt aaagaggagt aggtataaaa tgtttttgta gtttttgtga
     3241 agatagctta tacccacagc ggaagcgatg ataataaatg gcaacattag cgaagctcgg
     3301 ttggcagagt aactaccctt ggctaaagca gcgggaatgg gagaaataag tattccggag
     3361 agtaggatga ttagggattg actgattagt tttttttgag aaaaaatgta gatgattaga
     3421 ctgataaaac caactgatgc ggctattcca ataactccaa aatcagggat catgccgtag
     3481 gtggtttctc ctggtccatt agtgatgaga aattggggag ataagtaaga gaggtagctt
     3541 tgagcatatt tttgaatagt atagataact ttgttctgga gaagtcgaga aagttggtcg
     3601 ggtaggccgt tactggttag ggtaaaacgt tggtcagaaa catgagtcca tttgtcagtc
     3661 ggattgaaga tggcaatgtc ggaagttcgt gagccgtcgg gttggaatat aaatagtgga
     3721 gaacaaaaaa tgatgactag caatagaaaa taaataaatt tctttttgaa ctgaagaatg
     3781 gcaataatag gcaaaaagag aggtaggaaa actttggtcg aatgataggt ataaagagac
     3841 agtgagaata gtagggcagc taacaaatgt tggcggtaat aaaagattgt caggccaagg
     3901 atgattaagc tgacagctaa gttagcttcg agggcgattc gtgacatact aatagaccag
     3961 ggcgagacag aaaatagggc aaagctaatg atagggatgg tgttagattt tgggaaaagt
     4021 tttttgctaa gaaaatatat ggaaatgagg ctgaggatag agaaaagggc gttgggtaat
     4081 ctgacggcaa aaggattgag gccaaaggcc tgaattgagg ggatcatgag ccacgtctgt
     4141 aatgggggtt tgtagtcacc aaaggatttg agagaaagaa aattgctact tccccattca
     4201 tctgctcctg ttttgagaat agaataagca ctatatcctt gggcaagctc atcaggagaa
     4261 taactattgg gcaaattacc taattgatag aatctaagga agattccgat aaacaaaaat
     4321 aatgaaaaaa gtatttttat tttcattgtt tttcaataac ttgccccata atttgtccgt
     4381 tgggataata gactgtgtcg atggtacgat aaccttttaa tggttgtttg gttaggtcca
     4441 tatcgccagg gatatctttg ccttgaagca gataaaacac atctccggtt tgagctatat
     4501 tattgatctc atttagtgag tcggtgctgg gccaatggtt gataaaaaat atatttggct
     4561 ctattttgaa tcctggtaga ttgccgatga tgttttcttt ttctaagtct gagctttgtt
     4621 ggatgatgcg tgggtcaatc tgttggaaaa aagcaaagcg ttctagtgat tcatatttag
     4681 tgttgctgac aaagactcga gtggcattgg taggttggtg gacgaagagt tgatggtagc
     4741 cgtattgcca gtgagagaag tgggctttgg gatagtggac aaaatattca tgaagataca
     4801 tggcaaagaa tagagcactg atgatgatca tggctttgcg taatatcgga gacaaagatt
     4861 ctagccctaa ggcgataaaa taagataaag ggaataccat taaaaataat cgactagcat
     4921 gatagccacc accttgggtt agtgatgacg atatcggaga gataattaac caaaatagaa
     4981 aaagtgggtg accttttttg attccataaa ataaaggaat gaaaaggggt aagaggatta
     5041 gtccgtgtcc agggagattg tgacgtggat tgggatctcc agacaaaaaa attaattcgg
     5101 gtgaaaatga ttggagataa tttttgaaaa aatagttggt ataagcaatg gctttattgt
     5161 gggatatttt ttcgattaag ggagaatctg aggtggtgaa atcagtcctt tgatcgataa
     5221 ttttgttgat gacggccgga tcgttaaaga tgctgatcat tttaaaacga ttggcggcag
     5281 tgccagacaa aattgaaaaa attacgggtg atgtcaagaa tattccaaga agaagtggct
     5341 tgataattcg tgatagtgtg aagactcggt aatttttcaa aaagataaat aagataatta
     5401 aaggggtgaa gatgttggca atgttgtagg tataaaaagt gatggcaaaa gaaattgctg
     5461 agatgtagag tgatgaggtc ttattgtttt tgataaaaga tatgagatgg taaataccca
     5521 taagcataaa aaacgagagg gtggtcaatt cgaaagatgt acggctgtag tggatatgcc
     5581 aaggagagag agctagaacg ctagtactat agagcgctag aacgctagac ttagaagtga
     5641 ggagaactag tttgtaaaaa ataacaattg aaagtagacc gaagattagg ggaactagtc
     5701 ggacggcaaa agtgttgagc ccgaagatgg caatggaggg aacagtaaag tagattaggg
     5761 ctggagcacg ggattcagct aatgaatcaa ggtaaaaagg tagaaaatta cctttgatat
     5821 cgcgggcggt gtgaaggaga ctgtaggctt gataaccaac gtcgatttcg tcaccaaata
     5881 gaggtggaga tgaaattgaa taaagcctca caatagaggc aaggagaagt atcaataaaa
     5941 ataaaatgtt ttttttaatc acgctttaaa tagtctagca gaagccggaa acaataaaaa
     6001 attaaaacta gactgaagat acgaccaaga ataaagtttt gactgatgag taaattttca
     6061 acaaatggta gctgagggta gaaccataaa ttgtacatat taattaggtg gataaaagat
     6121 agtgaaaaat aagtagttaa gctgataaag ttgggtttta aaaatactag gatagtggcg
     6181 atagggaata tggggtaaag atatctttca tgcatattag tgagaaagat aaagctacca
     6241 aaggcggtga gtagggtggc ggctaaaagt gagttgaggt tgagctttgc tttaaagaat
     6301 tttagacaga taggcagtag aaataatgcg agtataattc tagaccagag ttgataagta
     6361 atcgggccaa aggtggtaaa ttctgaacga gaaaagtcga caccaaataa tagaaaccaa
     6421 aagttgaagg cgttgccgtt aagcatactc ccctgtcgga caagaactct attttcatag
     6481 aggtaccaca accagtcaaa gggtgttttg tcaccgaaag caaatggagt agctagaaaa
     6541 tagataaata caaaaaaaga gagtagcgga aaaatgaatt ttctccaatc tttaatttgt
     6601 ttgaaaagta atatggcgaa tatgggtaga tagataatta aagagagttt gaagaggaaa
     6661 ctgctaagaa ataaaattat tccaaaaaag tattttctat taaaaataag taaaagccca
     6721 ctgagagcta gtagattgat tagagagtcg gtctgtcccc aaatggtcga gttatagatt
     6781 acagcaggat tgaagagaaa aagagagcta gcgataatgg catagcgtgg tttgagatgg
     6841 ttgacgactt tgaatataag ataggctaag ccaatgtcgg acagaataaa ggggagcttg
     6901 accatccaag cgtgaagatt ttgttcgagg tatggaatta taaatgacgg aaagatgggt
     6961 atattttgat tgatgaactc tatactagta aagaagatct gtttgagata ggcgcagatg
     7021 gcaaaaagaa gcattgtgcc tagtggttgg tttggccaag aaactcccca gaaaatattt
     7081 tcataaaatt tctctggacc aagggctaaa aatcttatac cccaatcgat gtgattgttt
     7141 aaatcaggat gccaagtaac aagagacatt aataataggc gtaggaataa gcctattatt
     7201 aataatggta ttattttttg gggaagatac tttgccattt ttggctagac cagataataa
     7261 agttgatgac atggtgcata aaatacccgt ggaatttaat actactggga atgagacgtc
     7321 gccactggtc tttaggtgcg gcgacattgg tgccactagc tccatgacgg tggatgactt
     7381 ggcattgagg atagaaaaaa acctttttac ttaagtgcct tatctggcga caaaattcta
     7441 aatcttcgta atagaagaaa tatctttcat cccaaccgga gatacggtca taatcttgac
     7501 gactgatgag tatagctccg ccagagacag cccagactga aattggggtg tcggtctgtg
     7561 gtatgtattt gacgtaagct ggaatatgca accaatattc gcgaaaggca ttaagcggag
     7621 tttggggagg catgactgaa ccttgaggag ttaaatcagg attgagaaac atggggccaa
     7681 tggcgccgat atcgggatgt gtttgtaagt acttgaccat atcgacaagg gaactatcgg
     7741 tgagctccat atcggaattt aaaaataaaa aatagtcggc aatggctttt ttaacgccaa
     7801 cattattgcc agcagagaaa ccgagattag tagagtttct aacataggta atgtcgggca
     7861 tttttcctag gacggcgggg gtgtcatcgg tagagccatt atctacaata acaatttggg
     7921 caaatcctgc aggcaggtgt tttttaatag tttggataca ctttagagta atttgagcag
     7981 tgttccaggt agggatgatg atgctgatgg tcggtttcat tatttgaata aatcaaatat
     8041 ttcttggtcg gttcggactg gaaaaacaat tggaggagga ttgttcttga tttgtttttt
     8101 ggcagccagg atgatcttaa gataatttgg gccggatagt acgcgtaata cttgagtgat
     8161 acggttacta gagatcatct tggggtcggt gatattgcgc cagacagtca gaagtcgatt
     8221 tctttctttg atatagttga gtagtctgac agggaactta gacatagtag attcatgctt
     8281 gtgttcaata atgctggttg gttcccacaa aagtgtataa cctgacttcc aggcacgata
     8341 accaaggtca acatcttctg agtagaaagg ggcataaacc gggtcaaacc cgccgagttt
     8401 gagaaatttg gatttgtcga taattgaacc accacctgat aaccaaccac taatatgggt
     8461 ttttttaata tctaatccac gatcgtattg aagataaccg tctttccaga atatttttga
     8521 ccagttttcg tgtccaagtt cggaaaagcc gacaccaaag acttggggat tggatagatg
     8581 tttgtaggcc ttggtaatgt agcctttgtg gggaaggatg tcgctattta gaaggactac
     8641 gtaatctccc ttggcacttt tgacggcaaa gttggagttt tctccaaaac caacattagt
     8701 tttgtgagta attattttta tttttggatg ttttttttgt aggctggcaa tataggcgat
     8761 actttcatcg ggagaggcat cgtcggtaaa gataatttcg tcagcttcgg gagagttctc
     8821 taccacatga tggaagtatt ttttgagtaa attgacagta ttaaaattgg tgacgattac
     8881 ggagattttt ttcattgttt tattatcttg gaaaaattgg taaagaaatt ttcttttgaa
     8941 aagtaactac ttctggttaa agcatgatca gacatggttt taagggtgtt tggagaggct
     9001 gaaagtagct gggtcttgga gataagctca tcgatgttgt tccaaagata accttcttga
     9061 ccgtcagtaa ttatttcagg tagaccacct ttattgacaa gaagagggac agctcccgag
     9121 gccatagcct caactggggc catgccaaag tgttcggtgt tttcagggtg taatgattca
     9181 ttaacttcgt gcccggcggc atgccagtaa atcttggcac gactatatag tgactttagt
     9241 tcatcaaaat ttggattgat aagaaaaaca acaggtaaat tttggctgat atttttaaga
     9301 tgagagagat agtggttcat ggctgggtcc tctaaactac caccggctag gtagagtttc
     9361 cagtcgctat ttggattttg cttgataaat ttaataaagg cttcgatgag gatgtcttgc
     9421 ctcttggagt tgagtaagtt gtcaaaacgg ccgactgaca aaataatatt ttctttattt
     9481 ggatcggggg agaatttctc aacatcgact ggcggataga gtgtgtgaca ttttggccca
     9541 aaatgattgg tggcaaattt gtaagtaaaa tcagagttac aaataacttt tttgacaaag
     9601 atggttttta ttaagtttag agtttttgtc ttgaggttgg ttttttgaat aaaaggaacc
     9661 tggacatgga gcaaattatt ttttgaaaat agaaaaggaa tactccagtc gcttaaataa
     9721 aaaattaagt catattgacg ggtgactttg attctattaa tgagatcttg cacttttgcg
     9781 tgtggttttt ggggttgtct tttggagaca cgagaaatac tttctgaatc ctgagtgata
     9841 ttaatgtttt gtttttggtc ttgaaatata tctgggacaa ggttgacaga tgataaatct
     9901 agattgaaac gtgtttcggc ttttctgagt aagtcttgat ctccagacca aaataaatct
     9961 acttggtagc cattttttga gagtaattca gctatggtaa ggcaatatct ttcacctcca
    10021 ccaagagtat ccagataagg atcgtagata gcagctttta aattcatgcc ttttgttgtt
    10081 gtaactccca tagtctagcg taaatcataa aggtgttgaa tgtctcaaaa ataactgaga
    10141 tccagccaac agtgccgtca cgccacattt gttgttggac aaacctttcc caaaacttgg
    10201 tgagcatcat acgaataaac ctccaccaga caacgggtgg gtgtcctgat tcatgaagta
    10261 atctggcttc tatgtcggtc catttgatac ttttttgtag catagaagtt aagtctttat
    10321 gagtgtagtg aaggagatcg tttttaagag ctgagctggg gccgattatc tttggctgtt
    10381 cgtggacagt gcctgaatat gaaatgagag atttggtctg ataaagcctt ttgacgtaat
    10441 cagggtaagt acctccatgc tttactcgat gaccgaggaa atggttggca cgaggaaggt
    10501 caaaattggt gtattttccg ggtgagttga tagttgcaat aatctcttcc tttaattcta
    10561 aattaactct ttcatcacaa tcaaggtgaa gttgccatgg ttgagtagct gctttggctc
    10621 cagtgttgtg ccagagcgaa aaatcaattt ctttggatcg ggcatttata aggataatat
    10681 cgggcctggt tttcttggct aagctgatag ttttgtctgt agtggcttga ttttgaacta
    10741 ggattatttc tttggcaaaa ttaacacttt ttaaacaatc aacaatacta tcttcttcgt
    10801 ttccagcaac aataattaca gacagatctg ttttcattgc caactcccct tttgtctttt
    10861 acctaggtaa aaatcaataa caccacgttt ttgccatggt gtcccgcgaa caagcagtct
    10921 aattgattct cgaaaaaggg caaaattagt acgaaatgaa gcatattttg aggcaaagat
    10981 aagacggttt ctggtgagaa agtagtcatg aaaagctcct ccaccagctt gagatgagcc
    11041 ggagttgaca tgccaaatta cagatctcgg ctgataggca atcttaaaac catgacgata
    11101 aattttctga caaaagtcac tatcttcaca gtacataaaa taatcctcag gcataatgcc
    11161 ggtctttttg agaatacttg attttatgag gacacagcaa ttggtgatga actctaaatc
    11221 taatcgtggt gtatcgtatt gaccaatatc cacttcgtca agaccaatgt tgttggcata
    11281 gacattgttc cagtcaacat ctccaccagc ggaccaaata actttaccaa tatctgattt
    11341 tttatagcga tctttgtgga attcaaaacc tggggcaaaa tatatcttgg gtccaactac
    11401 gcctaacttt gggtctttat cgaatgtctt aacaagttca gtaagaaaat ttggatcgac
    11461 aacaacatcg ctattgataa gaagagtgta ttcaaatttg tgtttgaggg ctaatttgat
    11521 accagcattg ttgccagcag agaaaccaac attgtcaggt aaaaagatta agctgacgtc
    11581 tttatctttt tgatatcttt gggtaaatat atcgcaggaa ttgtcttttg atccgttgtc
    11641 gacgagatag atatggtaat caaagcggtt atgttttatt ttctttaatg aatccaaagt
    11701 gtcgatagtg agctggggtt gctgccaatt aagaacaata atggcgatct ttgtcatgtt
    11761 agaagttttt ggttaatctg gtaaggaaat gactaattct cattaataag ttcttaaaga
    11821 cgttcttaga gtattcttct ctaaagaatt tgttgaacca atgtccatac tcaaaaactg
    11881 cttcttggct ggccgaaaca aaagactctt tgaggagagg tttgtcaatt gagccttgtt
    11941 tttcccagag cctaatataa atgaggcaaa tggcaaatga ttgaagtaat ccaaaaatta
    12001 gcccgtgaat cccgtctttg tagccttcgt caaaaaagaa acggctgtta aattcctgga
    12061 ctggtttgag gaggaagtcg gatgtcttaa ctttgtatcc ggattgtttg agctcatctg
    12121 cctggatatt ggtgtagcgt aaggcacgag tgataaattg agtgactgat ggataggcgt
    12181 tgtgttggat ggctagcttg accgaatcaa gaagggtgag gccattgcct ttggtggaag
    12241 gttggctgtg aatttctttt ttccaggtaa cagagttctt cttaaagaaa cgaataaggt
    12301 aatctggcca agtgcgagaa tgtcggagcc atttgccaaa aataatattc ttgcggggta
    12361 tttttaccca gtcaatatcg cttctcttga tgatggagag gagttctttt ttgagatgtt
    12421 taccaagata ttcatctggg tctaataata atatccactt gccggtggct ttagagatag
    12481 caaaattacg agctggttca acgaacttca tccatctata ggggtaaact tgagcgccaa
    12541 actctttggc tacggcaaca ctattgtcgg tagagtgcat gtcaactacg ataatttcgt
    12601 tggcgaaatg tttaatggat ttaaggcatt tacgaagatg gtcggcttcg ttgcggacat
    12661 taataacggc agatattaaa ggtttatttt tcataaatgt agatatagtt tatcactttt
    12721 gaacttgata gatgatgctg tcaccggatt gaaaaatagt agtgaggaat aattttttgg
    12781 tatcgagggg aaatctgtct gtttgaacgc cagtaagata aatatagtca atgttgttgt
    12841 ttagaagaaa gccgcggtca cgatggatat ctttttgaga aaagaaatct agggaaagtt
    12901 gtcgcctagt ttggaaatcg tagccagagt tgttaagatt catctcgtct tccatgtaga
    12961 cagtttgatg tgaataggcg gaaacgtagg aagtggtctc gtaggcatag agtggtactg
    13021 gggcagttaa actatctttg atgtatttgt cgtaaggaac ggtaaggacg ataccttttt
    13081 tttgagaacg gagaaaatct agtgcttgga tttctggggt aggtaaaaat gctggaggag
    13141 caccgcctag ataatttggc atagcaccaa aaaaaccaag ggtataaaaa atgaagataa
    13201 cgacggtaac tatcttgccc cagttgtgtt taataactga attgataaag agggtgaggt
    13261 aaatattgct aataaaaaga gcgtaataaa tgaattggat agtattccaa ctagtcccct
    13321 gttgaataaa tagagtgggg atgagggtga gaaagataat atttagaaga agagaaacgt
    13381 taaataatct gggtaagctg ttgaggcgga taaaacctag aagtctaaag ccgtagtttc
    13441 cgactataaa aataataaag gagaagatgt agaccaaagg taggcggatg tcgaaagcac
    13501 tggaactttc tagtgtttgc ctaagactgg aaactttagg taaaaagagc ttgtcaggag
    13561 agtcgatcat agaattgata aaccaaaatg gtttgaaaac gaatagattc tgtgattcgg
    13621 ggttgtagat cctaaataaa ctaatagcca caactaggga taggacaaaa gtaagtaagt
    13681 gggaatactg ttttttaaaa aagacttgtc ggagagtgta cataaagtag acactgaaac
    13741 caacgacagc tgaataggcc ttggtaatgg gtaaggtgat caaaagtaaa aagaagatgg
    13801 catagtgact actttttttg tatataagaa taaggagtgt ggtaagtaag ataagggaaa
    13861 gtgtgtacgg tggattaatt tgattggatg gtgattgcat agcccagaat agactttcac
    13921 cttggaaatt accgtaacgg atcaaagata caatcgggcc aagagaagta gctagactgt
    13981 tgagaagaag aaggataatg ccgccagcat gcttgccggt aaccagttta cctaggatat
    14041 agcttaaaac aagcagagtt atggaaaaga gtattggtga taattggaat aaccatagtt
    14101 cggaagatat agaagttgct ttagaaacaa aggcgataaa aatgttaaaa aatgggtggt
    14161 aattgataag tttttctcct gcaaatatgg gcatatcaat actaaggggg ttgttaatgg
    14221 cgttgataag actaaggtgc cagacggagt cgtgaccgct ggggccccaa tagccaattc
    14281 cgtattcata agttaaacca ctacggataa ccggagtgat ttgcactaat actcctaaga
    14341 taagaaaaaa ggcaaaagtt attttttgat aagtagacat tttatttgtt ctagaaacca
    14401 cgagatttct tttctggaaa ataagtagct gtaggttaaa taaaaggtgg taccggtgat
    14461 tatttttaaa atgatgttaa gtagaggtga caggtggaga aaattaatgg aggtaaggat
    14521 gatgaccata aacaaagagg aaattaatgg ttggctgatt gttttgagag tttggattga
    14581 gagtagcttg tcggcgatat accaaacaat aaatgaacta gaaccaacta tggcggcagc
    14641 gatggatgtg ccaatatagc catatttaaa acttaagatt ggataaaata tccaagtgag
    14701 tactgtccac atgatcatca atttggtggt aatggtaatt ttgccaatgg cgttgaaagc
    14761 gttggttagg ggtgtggtga cggcggctat ggcaaaagaa aaagcaaaaa aataaagagg
    14821 aataatggca ggtgaccatt tagtatatct aggaatgaca ttgataatat caggggcaat
    14881 catagctatt ccagcaaggg aaggaaaaat aaaaagggaa atgtagtaga gacttttctc
    14941 aacagagcgt tttaagagtt ctttctcgtc ttgaattcta gaaaaggtag ggaaagtgac
    15001 tcgcataata gcatccataa agctaagagg gatacgggga cctttttggg cccatgatag
    15061 tatgccaaaa gattctctgc cgattattcc ggcaactagc aaactcgata gacgatcctt
    15121 tgtcatggca ataagtgagt ttagttgaaa gggtatgccg tatttgaata gacctctagt
    15181 ggcttctata gaaaatccta tacctatagg ccatgggcta aatctgtaga ttaaaataag
    15241 accgagtgtg ctgcgaataa cagtggcaaa agcataggaa taaacaccat agcccattaa
    15301 agcaaaaaga acagcaaaaa aataaaagct agtattttcg ataatatcga tggttgagag
    15361 taatttgaag tcaagttttc tttctagctg gactgaaggg atggttttaa gggaggcaac
    15421 aaaaaaggcg tagcaaagag agattagaat agacatttca gctaatccat aacctttgga
    15481 ttggctgatg ttgggataaa tgagagcaac agcagctagg gcaataagca cgaggacttg
    15541 ctggattaga aaagtagttc tcaactcagg gatagtgatg ggttgctttt tttgaattaa
    15601 agcagaagct agtccgacat ctgaaaaata gccgagtatg gcaactgatt cggcaacgat
    15661 agcaaataag ccaacttcac cagccccaag aagaatagtt agaaggaaaa aaccaatagt
    15721 ggatatggct tgaataccaa tgttgcgaag tgataaaaaa acaacactaa cggtagtctt
    15781 gtgcttgatg gaagctattt caacttcttc catatttttg gaaatataaa caaaacaccg
    15841 attaagaata tggcgctaag agaaatactg ttggcgacgt atctaattgg tgtatcgtga
    15901 agtttgacat atatttgatg gttgccagcg tcgaggccta gagatatccg cccaagctct
    15961 ggctcaactt ggaattctat tggctgtcca ttgttgttga cttgaaagtt ggggaaatag
    16021 agttgagaca gatagatggt agctggttta ctgagtgata aattgaagaa ttgccaatca
    16081 gtcccctttt tctgtccaga taactcatat tgagtatcag cgggaataat ttcatcaaga
    16141 tatggcttgg cttctctaag ggcggcgata cgggcagttt tgggtaaata gtcgtagatt
    16201 ccgctggtaa tttggtttct ccaggcaagt cctgatagtt tttgttcgtc ggtaatgggt
    16261 ccggaggtaa ctggagtgaa gtatgagaga ttgagaatta gtagtggcaa gataattaat
    16321 aaagaaagta attttttctt accaaacata agtgacaaga atccggctga aagtgaaaac
    16381 aaaaatacag taagattgag aaaacgccaa ggaaattgga ttttttgaat agtggtaaat
    16441 agtaaccaga taaaggttga tctctcgtgt gacatgaagg cggaaaaaag gcctaggaga
    16501 gaaatgagga tggcgacaag gtcggtacta ccaagacgtc gagtttttaa gaacttaatg
    16561 gcaatataaa gacaagctac taaaggaata atccagtgaa gatggccaat ggcaaatgac
    16621 attttatctt cggtgcccca gactgatggg ccatcacccc aatatctgct taaaaataat
    16681 tggggtaaag aagcgaagtg cacggaaaaa tggtagtaat tggtaaacat tgattcgatt
    16741 tgaaccaatt ttgactcaaa gattacgggc aaggtaaaaa aggctgaaag gccaaaggcc
    16801 aaaagaccag atatggcgag tttaatggtg gtggaaatgg ctttttgaat gtctttttta
    16861 tagttgacta taagccagaa gaaagaccag actaacaata caggagtaaa gatgagagtc
    16921 atggggttgt gtgatagagc tagtccacaa aaagataggg agagtagaac ggcatgtttt
    16981 aattttcctg tctcgataag ttttttagaa aagtaaagta taaaagggaa aaagacggcc
    17041 gcccaagctt cgttcatggc accccggacg tagatgttaa cggcatgata tggagcatag
    17101 gtgtaaaaca aagaagaaat aatcccgcct attgggccaa aaagtgaggc ggctagaaga
    17161 tacataccca aagcagaaaa aataatttgg ctagcagcag ttagcttgac ggcactgacg
    17221 aaagaaaaat ctagaagacg aaatgtttga ccgacaaggt agggcatggg tggataaaaa
    17281 ttgaataagg ggtatccgta gctataaccc aaatcaggta cccatcgaca gggtatttga
    17341 ccgtcttgta ggcatttttc catctctaat tgacgaatta tttgcatgtc gtcgtgcata
    17401 ttccaataaa tcccaggttt gagaaggaag aaaaaagcag ggagggtgag taaaaaaaga
    17461 aatataaatg gcagtagttt tgttttcatt atttagttta aacgataaag gactaattgg
    17521 tcggagtcgg gtttgctgtg ggactgaacg agagataact ttttgtattc ctcgctggtt
    17581 aactttaaac gggattgatt gaataatatg tagacttcgt caccatgatt tcgagcatta
    17641 ataagtttct ctggaatggt ggtaatatcg acaccaacac caaagacagt tagttgtggc
    17701 tggctgtcgg tgtatatctg gaggccgtca ggtagggtgc caaaataacc ttcagtaccg
    17761 acaatgatat tagctacttt tgatctttgg atgagatatt ggcttacttc tttgattccc
    17821 caacctgaag tccattgtga aacgtagccg acttcagtag aagatagttt taatttttgg
    17881 ggtgaagatg acatttgatt aattaagaaa atatttggaa gaaaacaaag aggaattaaa
    17941 aaataaaaaa ttttggcttt gacgtgagct agacctagag atagtaggat gataagtgat
    18001 ggaagagtaa agaggatata tcgagcagtg aaaattttag caacagctgc attcattaat
    18061 aatggaagaa accaccatga aaataagatt atgtatagtt gccagttttt acttttcttc
    18121 tttaaaatta aaaataatcc ggctaatgcg acactgatta atggaagaga tatgtagtta
    18181 tcgtaaatag aaaaaatatc gctaatatga ggtaatagag ggtctagggg gtgtcgagca
    18241 atttcagaga atgaccaaat ataatctttg ttacgcagag caatttggtg gaactgaggt
    18301 ccaaggcgaa ggatattgta aataataaaa cctagaatgg tagatataat aggaaaatat
    18361 atttttttgt agttattttt attaaaaata ataaaagtaa ggttagagag aacgacaaaa
    18421 tatatagctg gtgatttggt taaccaggcc agaccgagag agaatcccag tatgagggag
    18481 agatctagac ggggatattt ggctaaaagt acggagagaa gaagagagct ggtaccaaaa
    18541 aacgatagaa ggttgtcggg tagagcgagt cgatcaaaga aaaaggtaaa tggtaatagc
    18601 aaataaatga ctgagctaaa aaaagcgact ctttgactaa agaagattag agaaaagagg
    18661 tagagggtaa tgagagtgcc gcatccagcg agtatggaga taaaacgtcc ggaaattaat
    18721 ggatcttggg ctagtttaaa tgaagcagct acaagccaca tgaagaatgg ttgtttaccg
    18781 tcggtaactg gaacaaagcg gagtgtttcg acgttgcgaa ttagttgtga ccatctgata
    18841 tagattgcct catctccgaa aacaggaaga gagtcgagat tgtaaaatcg agaaaaaaga
    18901 tagatgggga taatgatcag aagtggccaa aaagacttca gtttaagcgg aatcatattt
    18961 tcctttgagg ttgttgacaa atattctcca gacttcttga gccatttgct tggattcctt
    19021 gacataacga gcagtggcgt cacctttggt gatgctggtg tcttcgtttt tccaaataac
    19081 agggacttct tttattttgt agccagattt gtgggcaatg aagaggagtt cgacatcgta
    19141 ggcggtaact ctccagccat tacctttttg aatatttttt atgactgaaa gttgaggaaa
    19201 tatttctttg gcaactgatg ttttgattga tttaaagcca cactgagtat caccaatatt
    19261 ggttttaagt aataaattac gtaaaactga gaacactttt gagcctaaaa ctcggatgaa
    19321 ggtgttacct tcacgatggc caccacgaga accaatagct atatcaaaac ccttgttgaa
    19381 gtaagggatg agtttttgta tctctttgat gggtgtcgat tgatccatat cggtaaaaag
    19441 agaaatagga tatttggcga cattaagacc attccagata gcgccgggtt taccaccttt
    19501 agggaagtca aagaccctaa atttcttgtg actgctggca aaatcttgag ctagcttgag
    19561 actattgtcg gtggactgat cgttacagat aattacttcg tattcaaact ttttttgtga
    19621 agatagaaat tcatctactt cttttaggac tcccctttta agattttctt cctcgttata
    19681 acaagggatg atgatgctga tttgaatcat atttgaatta tacgtcaact tccttgggta
    19741 tgtagaagct taaaaacttt aatgccgctg atctcccatt ctttatctat tttggcccaa
    19801 ccaaaggcgg caactgacca ctcgggatga ttgattggct ggcaattcat ttgaaaaggt
    19861 tcgcagacaa caaagagctg ctcggttatt tcgtcttgaa gtaatttgac tggggcatta
    19921 ttttcataca tgaagtaacg atagcctgga tcatagttca tcttggcaag aagagagaag
    19981 ttgaaaggtt gcccgtttga ctcattaatt atggagttga taattgcttt ggtggtatct
    20041 atttgatgag gcggttccca acggaagggg ttttcgagaa aggcaaagac aataagtatg
    20101 gtgatgccag taagggaaac aaatcttggt agattacgag agataaggta agtaaaagtg
    20161 atgaacactg gaggaaagag gaaggcaaaa tagtggtcgt agatatgttg cttatacaag
    20221 gctaatccgg taactccgct gaatatccaa atagacatgt atataaaagg taggttgtat
    20281 tttctagctt ttatttgttt aataatgtct gttagcaaga ggatggcaaa gattgagcta
    20341 acgacatagc ccaaattaat atttttacta gctagaaggc gggtattaat ttggttgaac
    20401 agctcaggaa taaggggtaa tgccttataa gctttgaggt tgacagtagt ttctcggtaa
    20461 gtaaagaatg aggcgatgga tttaatgttc tgaccgtcat gtttgatgtc gaataatatt
    20521 tgtggagtta aagaaagaat gaagataatg atagctaaaa agactggttt tagttttttt
    20581 ggtcgattaa gaagaaagat aatcgcggct ggtgggagga gaagtaaagc tagaaaatga
    20641 gaattgagac aaataataaa agctagtgaa gctaggacat agcgtgattt gctaacaaaa
    20701 tatataaaga gcagtgaaaa tagaggcatg acgtttgggt tccaggagaa gtttgaatat
    20761 tttatgacga cgggggaaat ggcatagagt agagcggcgg tataggcggt aaaagaacca
    20821 aaccaggaaa gagagaattt atatattaga tagacagtta acaccccaag aagggcgaca
    20881 actatggatg ggccgactgg attgaaccac gttaggagta gtccgggggc aacaaaaaga
    20941 taataataaa atggtccgag atacatattg ccgacactgg tttgtggacc aatgaaaaat
    21001 aagtcaccat ttttgaggaa ttctctgaca ataaccacat ctcgaccttg atcgccgagg
    21061 aattcggtat agtcacttat tcgataaagc ctaagaaagg cagctaaaag tacaatggca
    21121 atgataagcc aggaagactt gatacgtttg atcatttttg ggtaaaattt aattttatcc
    21181 aaagtctaaa gagatctttg aacccggcaa tgatgacatc gaggttggag ccagtagcct
    21241 caccggaaat gcgggcaaaa tggtggacgc cgacttcaac aatcttaaat ttagatcttt
    21301 tggctttgat gaggaactct gaggtgatga atggtcccct ttctgattct aactggggaa
    21361 tagtatcgat aactttcttt ctgactaatt taaatccaca atcaatatct tttactttta
    21421 ggccaaatag gaggaagacg gctaattgcc agagcttaga cccccagatt cgatagaaag
    21481 gaacttttcg accaaggtag taaccaatga ctaaatcagc tttagttttt tgttgagtag
    21541 ataaaagaat cttaatgtcg gagaaatcga attgtccgtc ggcatcggta aaagctatcc
    21601 attcgtattt tgagttgtag aaaccacttt tgaaggcagc cccgtaacca cgatttgggt
    21661 tgtgggtaat gatgcgaacg tttttgggat atttggcagt aatagatttg gcaatttgag
    21721 gtgttttgtc tttggagcca tcgtcgacaa tgactacttc ccatttattg gcgacttctt
    21781 tgattagcgg tagagccgca tcaaaagtct tttgcaagtt gtgttcttcg ttgtaacaag
    21841 gaaagaaaac tgatagttcg ttgatcttct tgaagctcat attttacagt atagcaaaaa
    21901 aagacttgac agaaagagat ttgttgatat aatatcctgt aatctttaat tattatcact
    21961 atgataatga caaataaatt aaataaaact ttattgagta cagccatatt gggattagtt
    22021 ttggcctttg tacctgcttc taaacctgtc tttggccaat attatggcgg tggtgtcgat
    22081 accccgtcaa ttgtagttga caaaaagatt agacctatag atagtagaac atattttgaa
    22141 aatattggaa gtgagcagaa agtttttact gaaggtgaac aaattgagtt caagatagtt
    22201 gtcgagaatc gtggtaacac tgtcttaaaa gatataaaag tcaaagatat tttgcctgac
    22261 tatcttaaaa caattgtttt tccgggtaaa tatatatcag tagataacag cattgaaact
    22321 acaattgact cattagagcc aggaagaagc aaagagttca ttatagtcgc taaaattagt
    22381 gacttgcccg ctagtaatta cgctggtgcg aaattacaga tgactaatag ggtaaaagct
    22441 acttcaggaa atgtttcaga cactgatgaa gctaaatatt ttgtagaaat gaaatcagtg
    22501 cctagtactg gtgctggtgg gatggtcgtc aatacactat tggcagtaac cataatcagt
    22561 tctgcttttg gattaagaaa gattgttaga aagtattaag tatctactgt aagtgtagat
    22621 atagggtcat cttgccgtca gggtggaata gtttaatgtg attgccaagg ctactttgag
    22681 cattacgata aaagtatgcg actccttctt taactgctct aacagttttg ttggtggaaa
    22741 ccatgtcgag accatggtgg aaacgatcgg catatagata agagtacttt gtgtgacctc
    22801 ggccctgggt gagagtgact ggttcataca taggccagac gttgtctttt aagtatggga
    22861 gcggatcgat atcggcagaa tagttccatt tagcaatatc gctttcactg aggttataaa
    22921 gaccaaaatg aagatgatcg ccaaatgagt atccggtatt gcccatcatc cccactgcct
    22981 ctccagcttt aacctgcctt ttaccgacga attttgctgc tagaagcgac tttaattcgt
    23041 cttcagtggc ttttttgagt tgttgatatt tgacttcgtc atttttggtg acttcaagaa
    23101 ggtttacttt ggtggatttt tgtttggcga gactggcttg ttgttctgct agcttctttt
    23161 gaaggatttc tagctcggcc tgtttaactt ttttttgttc cttttgatca ttgtagttgg
    23221 tacgaactgt ctccaggtca acaagggtgt cttgactacg cttttgtact atagaaatat
    23281 atttgtattg ttcgagaagt ccgttaaggc tgtcttggct aaaaatactt aagggcgagt
    23341 atcttttggc gagtttgtag ttttgggata cttggtcaat aaaaattgaa gagagatggt
    23401 taagtgatag atctaattcc cctattttgg tggttagaga agcaatttct gattctaatt
    23461 ggttgatgtt tgtctctgtc tggttgattt tgagaagggt tagctcgacc tgggagttga
    23521 gaagttttat ttgagtggtg agtgtgtctt tggctttacc gagttcagtt attttgccgt
    23581 ttagttcttc aattttttga gtaagagcat caacggcttc ttgagcaaaa gaactgatag
    23641 aaaaggagaa aaataggatg gtaatactaa aaagaactat gagtttattt ctcataggct
    23701 agaatttgat atatcttctg gaaccgacga aactagcaaa ataaccgaca agagacccta
    23761 gagcaatcga tccagtgatt aaataaaggt aaaaggtgga gtcttccgaa atgaatatga
    23821 ctggggaaaa gaattggttg actggttggt taaagtaaaa agataaagcc agggtagtta
    23881 gtgagccaat ggctcctccc caaaaaccat aaaataatcc ttctaataag aagggttttt
    23941 tgacataaaa ttttgaagca ccaagaagac ggctgatacg aatttcatct tttcggttgg
    24001 tgatcttcat accaattatg acggcgacaa taaagaagga tagtgtcgcg gagacagata
    24061 caagaattat gccagcctgt cggataaaac ttgtccaagt gattagagag tcgatgacat
    24121 ctttttggta aatgatctct tcaacttgat cttttttggc agaaaagttt ttggcaattt
    24181 gttcgagaac tttcggatcg gaaactgaaa cttcaaaaga agccgggaga atactggcgg
    24241 tgaccatttc agtcaacatg ggattgtcgt tgttttgttc tttatatata gctagggctt
    24301 tttccttgga aatgaatttg acctctcgga tactgggata attggcaagt tctttttgta
    24361 agctctcaac agtattgcgg tctagaccgt cttgaaggaa gatggtgacc tctggtttgg
    24421 tctcaaaata tgagagtatt gaagatagtc cgctactaac cacaataaaa gtggtaagac
    24481 agaagaaagt ggtggtcata actaacaccg caacaaagct ttggataggt gaccttctga
    24541 tgtggtgcca ggcgttgttt agaggtgtca ttttgttttg ttttttttaa cttt
//
LOCUS       DDWP01000010            3270 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_29974, whole genome shotgun sequence.
ACCESSION   DDWP01000010 DDWP01000000
VERSION     DDWP01000010.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_29974"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          969 a          910 c          396 g          995 t
ORIGIN      
        1 acttgtcaca aaaatattta gaccacctcg gcatccccca acaaaattgt tggtccacca
       61 aaaatgaaca aatatatttt caagataaaa ttatcagcgg actaaccctt cgtcaacaaa
      121 atctattgca attactaata aacaattacg gacaaaaggt ctctctagac gatatcgcca
      181 aaatcctctg gcctaacgac gaagattatt ccctctgggc tatggtcaaa gaaatcgccc
      241 gcattcgtaa atgtctagtc aacaacaatc tccccggctc ccttatcaaa tcatatcgaa
      301 agataggata tagcttaagc taaaatagcc taccctgttt ttcccatcat atttctaatt
      361 ttatctctct caactactgt gatctttgga aacggactaa gcccaggcca ccttcctttc
      421 catttaggtg atgttaatac atcaaaagtt tccctaagaa ttatttgatc aacataaaat
      481 ttccattcgt catccgctaa acgcaccctc cgatttgatc ttggttttat tccacctctc
      541 attggctcgg taccaccagt atattcgtct tcaattcctg tagatagcat atccccaatt
      601 ttacttatgt aatttttctt atcttgacgc ttttatgaca tcaacaccca cccttctcat
      661 tacgcacttt tttgaaaaac ccgctttgta cacaagctcg aaatttgagc tactctttca
      721 taattcgggt agcctccgtg accaattaga cttacccttg tctttttacc aacccgttct
      781 atcatcggag taactgactc ccacccactt aactcacaca ccaaacgaat tgccaggttt
      841 ttatctctaa attcatgctg ggtataaaca taatcatcac catccacagg agatactggg
      901 tatctacgac cagcaacatt tatttcagcc ataattttaa ttaatacaat cttttaaagc
      961 accagattat atcaccttca tcgtaaaccc atgcgtcaag gccaccgcca ccgcatccga
     1021 agcatgggac gggctaatcg attcacttat ccccaattcc atttttacca ttgcctccac
     1081 ctgctctttc tccgcccttc cataccccac caaagcttgt ttcacctgca aaggtgtata
     1141 ttccgccacc tctaaaccat tctttttacc acacacttta atcaccccaa ttgcttccgc
     1201 caccttcaaa gccgaactgg catttttagc aaaaaacaaa ctctcaatcg ccatctctgt
     1261 cactttatat tctctaataa ctttttcaag ctcattataa attttctcca atctatccgt
     1321 atttttgtcc tttttgtccg tcaatattac tcccgccact accagcttac cttcgtcaac
     1381 aattccataa ccagtgtttt ctaacccagg atctatacca agtatcatgg cactaattta
     1441 acattatttt ctatgcttcc atcatcataa accactactt ctgtaaacct tggcgtctgt
     1501 gtatttcgtg ccgccgaaat cgctcccacc aacaccgtct gccaattatt aattgtataa
     1561 ctcgaagcat aatgtaagtg ccccgagtat cccattttaa ccccactact aactaataac
     1621 tccatcaacc actccgcctc acccgtcact ttctgactat tctcacccat gatatgagtc
     1681 gaaaaattgt gagctagggg cttatgcata aaagccaaac aatatttaat taaacattca
     1741 gatacctcgt cctcaatcca agacttttga tcatccccaa gccctcgcca attactatta
     1801 tcaatcaata taaatttcca accactcttt tcaaatgact gatacttttt cccaaaaatc
     1861 caaacatttt tataaatatc gtgatttcct ggtactacat atacattctc attcaacaca
     1921 ttttttattg cctcctgttc cgctctcgtc ccatcatttg tcagatcccc tgtcacaatc
     1981 accaaatcac catctcccat atcaatagcc ttttccaaca tctccgtatc gttatgaaca
     2041 tctgacatga ccgaaaattt taaaatttct ctttttggtt ctattcttgt ttctatcttt
     2101 ggactttcga cttttagtcc gccagctggc ggatcagact ttcgactaac cgacaaatac
     2161 cagaaaccaa gaacaatcaa aacaattcct aatccaatca atatcttctt cacaccccat
     2221 tctaccaacc tttcattcag gcatatagtc tttacgaagt aaacaatttt taggagcaca
     2281 gcttctggag ccatttatcg gcgaagcacg cgagcaccgc aaaaatttgt ttacgagtta
     2341 aaagactata ggctactttg ctccctatcc acttatccac atccataccc catattactt
     2401 tactccaaga caaacatatt attttacaca ccaaaatagg tcatttttag aatttagaat
     2461 ttagaattta gagtttaagt tataatactc tatggacttc tctgacttta aaaaaaatgt
     2521 ccctcttgcc ccttatacca cccttaaaat cggtggatca gccgaatatc tattcatcgc
     2581 taccaaacaa tccgaactat ttgatatcct aaaattcatc cacctccacc ctgaactacc
     2641 ttataccatt ctcggacgag gcagtaatgt tgtcatctcc gatcttggcc ttcccggcct
     2701 aaccatcatc aatcaagcct cacagatcaa agtctccccc aaaattactc ctgccacgtc
     2761 ctttaccgcc accgacaccc aaagaaacga aaacgaacct agtcaatacc tcgactttag
     2821 caagattgat tatgacgaat ctcatctccc aacccagtct gtcaccattc aatctggcac
     2881 ccctctaccc tacgccatca gccaattaat atctctcggc ctcactggcc ttcaatggtt
     2941 tgcttatatt cccggaacta ttggtggcgc tgtctatcaa aacatccatg gtggtaaata
     3001 tcacttttcc gactatcttg agtcaattaa agtttttaac ctaaaaactg gcaaaactga
     3061 aatatataaa aaatcccaac tatcttggaa atacgaaaca tccttttttc agacaaatcc
     3121 acatctgatt attctctccg ccactctccg tttatttaaa ggaaacacag atctagccaa
     3181 acaagttgtc cgtgcctgga tcgaacaaaa gtcaaaagtc caacctatca actctcctgg
     3241 gagcgccttt gccaatccat ctttgactga
//
LOCUS       DDWP01000011            2791 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_18280, whole genome shotgun sequence.
ACCESSION   DDWP01000011 DDWP01000000
VERSION     DDWP01000011.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_18280"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          884 a          653 c          453 g          801 t
ORIGIN      
        1 aaatccagac atctggtcta accaggtcaa ggccagtgat ataatgcaaa aactatctga
       61 agtcgataag agtataaaaa ctattaactc actagagtca cagatcaatt atctaattga
      121 gatagacagc cttctcagta ccgactctga cgaagcactt tcccaacaat cagaagctgc
      181 catcagacaa gtcaccaaag agcttggaga tttagagtta caaacctatt tgtccggcag
      241 atacgacaaa aactctgcct tgctctctgt ccattcaggt caaggtggta ccgaagccat
      301 ggattgggct tcaatgctgc aaaggatgta tatgcgtttt ttcgaaagaa aaggctggcc
      361 ttacgattta ctagatctaa accccggtga agaagcaggg ataaaatcag ttcatttcaa
      421 aattaatgcg ccctacgctt atggttttct aaaaaaagaa ggggggactc atcgtcttgt
      481 cagactttct ccgttcaatg ctgaccagtt acgtcaaact tcattcgcca aagttgaggt
      541 cagtcctttt atcgaaaata cagctgaagt agatcttcgc cccgaagatg ttgatttcgc
      601 tgcttatcga gctggtggtc acggtggtca gaacgtcaac aaagtcagta ccgctgttcg
      661 tctcgttcac aaacccaccg gcctcactgt ctcttgtcaa agccaacgct cacaagacca
      721 aaaccgtctt atcgctatgc aaatgctcag ttccaagcta tgggctatag aacaagaccg
      781 cttttccgcc gaaaaaaaag atctcaaagg agagcatgtt attgccggct ggggacatca
      841 aattcgttct tatgttctcc atccttacaa gatggtcaaa gatctacgca ctcgatacga
      901 aacttcagat actgtcgccg tcctcgacgg tgacattgat aaatttattg aaaccgagct
      961 aaagctatga gctttatcaa tttaattata ttattcattg gtcttgcttt taaatcttta
     1021 agtcaaccaa cattattacc tgattttaat tttctcatcc ttggtctcga cccaagaaat
     1081 gactccttag aacaaacaca agttaccgat acaatcatcc ttggtcgttt aacttccgat
     1141 gcccaaataa aaataacttc tctgcctcgt gacctctgga gctactctct taaaacaaaa
     1201 atcaaccaaa tttatccttt atctcttaac gtcgataatc agtacgattt tattcaaaaa
     1261 aattttcaac aaatcactgg tcaacctatt aatcggactc taattcttac cacccaaaac
     1321 ctaatcgatc tagtcacaat cttaggaggt gtggatgtcc atcttgaaaa aggtttcatt
     1381 gacaatcaat atcccaatcc tgaatatatc aagaatcctt ctccaaaagt ccctgtctac
     1441 aaaactattg aattcgcttc gggaatcaat caccttgatg aatcaaatat taccgagttt
     1501 gttcgctctc gcaaaagtgc ccagaccgct agtgaaggtg gcaccgatat cggccgaaca
     1561 gaaagacaac agctacttat tgatgccatt attaacaagc taaagtcacc cgatatactc
     1621 tctagttccg ataagatcct ttctctctac aatttctatc accaaaatat ctctaccaac
     1681 ttcaccgatg ttgatatcgc tagcctggca attagaataa acagacattt tctcaaatca
     1741 tcaattacaa aaataaccat tcctactggt gaaaaccccg agacagacat actttattac
     1801 ccaggtaaac tcaccctagg acaatgggct tttatccctc aagatcaaga ctattcacgc
     1861 ttccacctgt tcctccatca acatttctca ccatagctta atttgctata ctgaattatt
     1921 aactattaat agttctatgg atatcccaaa tctagctcaa cctaccatta ctccatcaaa
     1981 aaaattaatt tctctaccaa ttattttaat aatctcagtt ttggccatta ctagtggttt
     2041 ttggttatca cgtttccttc ccaccactag ttcttcaacc actgattcaa ataacagtct
     2101 atatagcaat acctcttcaa cagaaaatat ctctgagcca gaccaactca aggcgggggt
     2161 agtctatggt aacaacgatc ccaattgtaa agacactgcc actggtaccc tcgaaaaagg
     2221 tgatataaat ggcgaaggga cacatatcct gaatcgtcca ggtggtaaag accagagagc
     2281 ttcattaaca tcttctgttg ttgaccttga tctttttatt gataaaaata ttactatcac
     2341 tggtcaaacc aatgcctcca aaaaaactgg ttggttatta gaggtttgta gtgtcaagat
     2401 tagcgaacaa taagatgaat gtcaccttcg ataaagtcag taaaacattt ggtaccatca
     2461 acgctcttaa cgaggtatct tttgagataa cccctggaga atttgttttc tttgtcggcc
     2521 cctctggtgc tggcaaatca actattctta agcttgttct caatcaatat ttaccgactt
     2581 ctgggcaagt caccatcgac ggaacttctc tctctttaac caaaaagaaa gccatcgatc
     2641 agtatcgacg taaaataggc gtaatttttc aagattatca actaatttac gataaaacta
     2701 tcgaagagaa cattgccctt gccctagata taaacaacta cccttcagcc aacatcccta
     2761 gtcaagttga aaaagttatt gagcaagtta a
//
LOCUS       DDWP01000012           18509 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_30565, whole genome shotgun sequence.
ACCESSION   DDWP01000012 DDWP01000000
VERSION     DDWP01000012.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 18509)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18509)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18509
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_30565"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    7113..7122
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5731 a         4990 c         2050 g         5728 t
ORIGIN      
        1 ccttaccctt cttacccacc cttttttgca caaaccaaaa cggccaacca ctatcaatca
       61 ttatcattaa tgacaaagtc gccaatagtg gagataataa aactattaaa caacaagcag
      121 ctaatctttc caacatcatc tttgtattct acatcctagc gatgcctacg tttggtgtag
      181 ctatcgattt tcaagaatca ctcattttct atctcatacc accaattatt tatctaaatc
      241 tattgagcga gaaaaaaacc acatcccccg tcaaccctcg tattatctta ctgcttattc
      301 tgtctctgtt tacctacctt gttagtactc tgtattcaaa aaactttggt atgtcttatt
      361 accatttctg gaactatttt atagtcgttt ttttattact catttatctc tattacaaaa
      421 accatttcca caaaacattt gaaaccagcc tcattattat ctccgttatc tattgcttga
      481 tattttatgg tcaaaagcta ggattattac ccccagacgc taaaaatttc ggtgacagtt
      541 ttctctccca ggcatggggg cacagttacc ttggaaactt cctaattatc agcactctac
      601 tctcaatcaa aaacatttcc accacaaaga tatactcctt ctttaccccc attctcttta
      661 taaacaccat ctttctctct tactcccgct cagccctagc cgccataatc ttggggcttt
      721 tcttattaaa atatcccttc aaaaaaatcg ttattcttac cactatattt ctattagtgg
      781 ttatgaccat aaaatccgcc atacccggac aagaatataa aagcctcgat ggtaagcgcc
      841 cggaatattt ctaccaagga atagtcggat tcatctcccg accactcttt ggcaacggac
      901 cgggcacctt taccataacc aacaatcaat accgcatccc atctgatatt ggtaccaaca
      961 ctacccacaa ttctattatc gacaatttac acaatcaagg gattttctac accattatct
     1021 ttagctgtct actctacatt ggatttaaac atcaattcaa atacaacttc ccctattttg
     1081 tcataggctt ctccagcttc tttggatcac tacttgattc atattggtca aactatggga
     1141 ttttactcat cagtcttatc tttattttct accaacctca aaatacgact caaccagccc
     1201 acaacaaaga cagcaattcc cataccaaaa ttctcagttt tagcaccatc attctgctat
     1261 tgtttttcat atccaaaaca acttccgatt atttgttctt aatcggcaaa tacgaccaaa
     1321 gcctaacttt cgaccccctt aacctaaatt caagactaga attactcaac gatccccaac
     1381 atcttcccac tactttacgc cttttccccc aagaagacac tgtataccac aaactagtca
     1441 atactgttcc cttaccccaa gccaacaact attatctaga atatataaaa aagcaccctt
     1501 atacttcacg tgactatcaa ttaaagctac tatcctccgc caatcaatat cacgaccagc
     1561 aaatatttaa cacctttgaa cccttctacc cactcctttt aaaaaaacct cccactcatt
     1621 ttgagtcgct acaatttgct aaaaactatt atcttcacgc tctccatctc tggcaaacag
     1681 acaataaatc agcgtctctc aaatattttg aaaaatcata tcaacttgcc aacggccaaa
     1741 gccacttctt tatagaatac gccaacgccc tttaccacaa taattatctt tccctatctc
     1801 aagagataat taacagttgc ctaggaaacc catatgccag gattcattgt caggaatata
     1861 cttccaaata ttcaacaact ccattacctc caggagaata taaggacact atcctcagtt
     1921 tataaaaagc atatccacca actgctacaa ttctgtcaat gtcacttttt ataatcttcg
     1981 attccatgaa catagggggc attgaaagaa agatcgtcga tatttgccaa aattactccg
     2041 acacccccac caaaatctac ctactcctta agacaaagtc aggagtcctc cttgaacaga
     2101 ttcccaaaaa cgtaattata ctttcaccca attatcatcc atttatcagc aatctattct
     2161 tttgggtgtg gatatacacc caagttaaca gatactcacc caaactaatt ttatcgtttg
     2221 gcaacttcta ttcgatacat actctttttt caaaagtcat ctttcacttt tccaccccca
     2281 ctatcatcag cgaagactct agtatcgaca tccagctcca atccgacaaa tttacccata
     2341 tccggagatt actcatcaag cacctttatc ctaccgcatc gggagctatt gtcctcactt
     2401 ccacctccaa gaaaaaactc attcaactat gtcctaaagt aaaaaacatt atcaccatac
     2461 tccctaattg gctaccactc tctctacccc atacgccatc tgtcaaaaca accaaaacca
     2521 acgacttact ctttcttggt cgctttgcca cccaaaaaaa tcctcttgaa tttctcaaaa
     2581 tctaccgaca cctcctcctt tcccgaccca agaccactgc caccatggtc gggtcaggac
     2641 ctttactcac tactgtcaaa aattacatca ccaaacacaa tctaccagtc aacctaatct
     2701 ctcctaccat cgacgtctta ccctactatc gtcaatcacg tatcttatta ctcacctctc
     2761 tacacgaagg tttccctctc accatcctcg aagctttttc ccaaaaatgc ctagttgtca
     2821 gccggagcct acctgaaatc cgcgacttct ttgatcttaa ttcccacacc actctgtacc
     2881 aatcccctac caaagctcca aatatggttc aacatctatt aacccaacct cgcgaatcta
     2941 cccgattagt caatcactat tacacacgca tccttaacga ccaaaaaaac aactttcatg
     3001 ctactttaaa ttatctaaac caatacttat gacaaatcct cgatctcctc gacttctcat
     3061 ttcttttcat gacatggcac ttggtggtat tcaaaaaaag atagttgata tctccaaata
     3121 tctgataaaa caccaacccg acaccaatgt cagaattagt cttcgtcgca gaattggtat
     3181 ttttattaat caaatcccct ctaagaccaa gatctcctca ccataccgac ccatatatcg
     3241 tttagggggc attcgttttt ttctttggat ggtcaaagaa attctcatct tcagccccac
     3301 tcatattctt accttcatgg actttagtgc catccccacc attgccgcag ccaaatttgt
     3361 tttctggaaa aaaattaaaa tcaccatcgg cgaagatatc ctcacttcaa agtatctcga
     3421 cgacacttac tcaaaagctc ataaaagatt aaaacaaaat ctaattcaaa aatactatcc
     3481 tcaagccgat tccatacttg tccagacacc agtccaaaag aaagatctag aaaatatact
     3541 caatctccca aattctccca aaattatttc tactcccaat tggcttccct tagactatcc
     3601 ttaccaggaa acatatatca ataaggacat tgatattctc tatgttggtc gtattgccgc
     3661 tcaaaagaac cttactaaat tcattgaaat tatcaaacag atttcccata aggatccaca
     3721 tataaaagcg accattgttg gttccggaga agaagacgat aagatatccc aactaattaa
     3781 aaaaaataac cttcaaaaaa acattacccg agttcctcag atccccaacc ctcaagacta
     3841 ctatcttaaa gccaaatatt ttctactaac ttccgactat gaaggtttcc ctctaactat
     3901 catggaagcc atcgcctctg gctgttatcc tgtcagctac gatttacccg agataaggca
     3961 gttttttaat aaagacactt caagctatat ctaccaaact aacgaccaag cagttaaact
     4021 tctcagttca cctccaccaa aaagcttgtc ttactaccaa tccaagctaa aaaaacttca
     4081 acaacagcac atacaaaaat acattgattt ttgtcttaac tagtctccgc tcccaacttc
     4141 cccgttaatt tatattttgc atatcttttc atatcatatg ctgttgcccg atacgcgtta
     4201 catccgctat tacatttctt acaggctttt ttgggtagat ttttacgaaa ttcgatattc
     4261 tttggatcat aatatatttc ccccacagtt cttttttctt tgatgaagtt acctatcggc
     4321 ctcaccgaca gacaataata gacatccccc aaactatcca ctacaaaatc atcatataag
     4381 aatggacaag gagttgtcct attagtccca tttttataca tacttatcat atcctcccag
     4441 taataacgac taatacctct cgtactcttc gaatctctaa ttgcttctaa cacctcaata
     4501 aacttttctt tcacattact atcaatatct agctctcctt ttgactccat attttttata
     4561 taagtgtcat gaaaaccaac tacctggaaa ctaccttctg ttttactttt ccttagccac
     4621 tccaccatct cctgatacct atcaatattc tttttcaaaa gcaaactcga tacagccacc
     4681 ttaaaaccat atttactggc cagtttttta taacccgaca ctgtcttggc acatttatta
     4741 aaaccatttt ttattctacg aagactatcg tgaacatcct ctaccccatc tattgacaca
     4801 ctagctacca acttaacacc ctttctttta cagtacttgg caatttccgc catactctct
     4861 attaaaatat ctggggtaaa accgtttgaa tttatcacta gtctttctaa ttttggcatt
     4921 gtatcgacaa acaatttcac cgccaaaaca taatcagcaa acagtgatgg ctcccctccc
     4981 gagacaatta aatctcgcac atctctaaat atcgggtctt tcattacttc cacccaatcc
     5041 ttatagacta attcattttt aggtctcatt tgccaaatat tacacatcac acaccgcgag
     5101 ttacatttat aggttaagtt aattatcaag cttctcggag ccaacacttt catctttgat
     5161 tcaaagttat agcgatatac cccttcaatc aaagaccaca atttctttag ataatccatc
     5221 aattcattat ataatgacct atgttcaaag ataagctcag aaaaattaag ccaatcgaaa
     5281 tatttgtttt tatatttacc ttttcacttc tagtctttgc cataaaattc tacaccttca
     5341 agccagaata ccgaattatt cgagtcgaga tagtcaaaca aaattggata gaaaactaca
     5401 acccactagg ctataaggct ccattctggc ttgcctcccg gttagagatt ggccaatcag
     5461 aaaaaaatca tagcggtcag atcatcgccg aaattattga tctagaaaat tacgaaaaaa
     5521 atactgaaga aattgaacta tttctaaatc tcaaaattca agttgctcac aatcctcgtg
     5581 acaattcttt tagtttcaaa ggccgccctg ttaccattgg ctctcccatc gaactttatt
     5641 tcaacaacac taccttagtc gggcaagtta tcgatgatca agtccctgaa agtggatatc
     5701 cccacaaaga cattttagtt accctccgtg gccgtcagat aaacccagct gtatattcta
     5761 aaattgttcc cggaatagaa gtatccaatc taggcaccaa cgaggttatc gccaaagtca
     5821 tttccgtttc acaagaaccg tcaactctaa cctcagccaa actcgatcag tacagacagg
     5881 agcttctctt ctcaaccaat ccaaaacaaa gagatatcgt cattgtcgct caaataaaag
     5941 ttcaacagtt agccggacaa tggttctacg ccggacgaca aaaagttaag ctttccgcca
     6001 atcaagatca caaactatgg ttatatacca aagacattac tctttacgat ctcgaaattg
     6061 aagacattaa agaattacct caaaaataac aactatttag ttgtcttctc tgcatccttt
     6121 ttcctcctgc tttttgttgg taatgttaaa ggcttggtag ttatttttgc catcatcttt
     6181 agtcttttct atttctttac cgagtccgac attctcgaaa gtactctcct aacccttcta
     6241 gtatctctcc cctttgagcg ccacttacgc cagtgggcac tatatagccc atttatcacc
     6301 gacactagtg gttattattt ttactttggc atatccatca agcttttttt tggtttagtt
     6361 atcttaatgc tgcttttgac ccccaaagga cgtcaatttt ttaaaggtgc cgttctaaac
     6421 aaaacatctc taactcttct tttctttttc actctcgcga ctatcaatag cttattaact
     6481 tcccaaagat tgatttttcc ttttaccggt tatgttcgac tcggatttgc tattttcctt
     6541 ttctacctag gacaatcatt ctggctaccc aaaaattacc acaaaatatc tgcctacttt
     6601 acagcactat ctttcataat tattttcttt agttttagac aaattattct ccaacacccc
     6661 attgggcact ttctcgaact caccccctct ttctcacctc aaggttatta caccaccgat
     6721 ggcatacctc aataccgagt cgccggattc ttttcccacc cagtctattt tggcagcttt
     6781 atctgcctta tcatccctgt ctttcttgga cttcaacgct acaaaccatt actctctctc
     6841 cccgtcatcg gtcttcttac agtgtccagt ctcagtaccc aaagtcgttc agtttggata
     6901 agcctactat ttcttctgat atcctacttt gtgttttttc tcaaaagtaa caagaaaatt
     6961 ctcctcacaa ttttgtttgt atttttaact atctttacca tcctgtctct cactagacta
     7021 agttcaatca caaccctgtt tgaaggtggc aatagtggca ttgccagatt aaatcatcta
     7081 tatcttacct ttgacatctt caaatctagc ccnnnnnnnn nnaccaacta ttttgccgcc
     7141 ccacctcaca acaccatcct tattttccta accgagcttg ggctacccac cacactccta
     7201 ttcttagcat ttctaaccta tctactttgg cccaccacca atatctttcg ttggccaatt
     7261 ttaaaatttt cactttggct atccctaata acttttatta tcagttctca attccaccca
     7321 ctcttcaacc tcgatcccac atttgagttt tttatgctca ttgccggtat atattcaaaa
     7381 atatgcgcca caccacaatc aacctaacca tcgccatcgt ctctcacaat cgtccagaga
     7441 tgctaaagaa atgtctaaaa agtgttagac agcaaagtct tcagccacat aaagtcatca
     7501 ctatcgatac caactgcgta cctctttccc gaaatattgc tattcaaaaa tgcaagacac
     7561 gatacttagc ctttgtagac gatgattgtc ttttagacat ggactgggtc aaaaacgcct
     7621 atgctagtat aaaaaaaaca tcatccacct tcgtcattgg ccagtcttta ccttcgacac
     7681 cgctcaactt tatttccaac ctacaatttt ccaattatag agattggttt aacaaattga
     7741 tcgccagcaa tgatcgttat gccctcaacc aggctactga taccaaaaac ataatcttcg
     7801 actatgacaa aataaaaaat attcactttg acactcactt tacagttacc gaagatatcg
     7861 attttggcct tcagctcaaa aaaaacaaac tttacggcca ccattgcccc accatggttg
     7921 tccatcacca cgaaaattac tcactacttc aacttatcaa aaaaaattac cataaaggtc
     7981 aagacaaaaa actcctcgac tccaagtggg ggaaatttga cgactatcaa ccacaattat
     8041 ttaccctaga cccaaacaag atatcttttt cactcggata tatcttcaaa aaagccccat
     8101 taattactat cgtcaattcc ctagacaaag gagccaacgg atatagagcc aaaaaatttt
     8161 accaacatat aaaatcaaac tatcataacg tctgtctcta caattccaat cgtgactttc
     8221 aaaaacaagc cttcgatccc agcaatctat ttctttatgg accatatttc tttttatttc
     8281 acctctttca gtttttaaaa tatattctcc atttcgactt tcctacccac cacttttatt
     8341 catacctcaa acttcgtgga gccattgtct cacgacatct aaggcagaca aacaccactc
     8401 tagccatcct ccaataccca gaagatatgg cctcaattac caagtcccaa ccatataaaa
     8461 ctctctacga ctctcctacc attttcagtc aagaaattga aaaagaccct cgatatagtc
     8521 ccgccatatc acaactagaa aaaaaatctt tcctcttatc tgactttgtt tcttttcatt
     8581 ggtcttccta ttttaattta ctacacgata tcaatattca tcccatcaaa gaaattaccc
     8641 tcaattgggg ctgcgatctc tcctcacaga ccacctcttt ttcgcctaag cccagaatag
     8701 tttatttagg tattcttaat tctccttggg tcaaccccca gctacttatc gacctttctc
     8761 aaaaatttcc tatcgatgtc tacagctatc aacaaccaga tccaaaaatc tacccgcctg
     8821 gaagtatcaa ctattgtgga taccttaaag acttatcaga attatcaaaa taccaatttg
     8881 gactaattac tatcacccaa gacaaattaa gacaacaggg cttctctgcc aaacatcttg
     8941 aatatctcaa ccatggcttg ccagtcctct gccccaactg gagaaaagat attctgttag
     9001 caccatctac cattagttac actccaaaaa actttctttc tcaggtccac agatattcaa
     9061 aaccacatct ctggcaaaaa aaacatctag ccgcaattaa cctcgccaaa aaactatcct
     9121 ggaaaaataa tcttacttcc cttgacgaaa taattcgccg gccctagtca tcagcccgat
     9181 caacaaaccc aacaccaaat tccccacctc ccacaccacg taaaccaact ctttggctgt
     9241 attcaccgca ctaaaccaaa aatcactaat cttgatcttt aacctttctc ttagtctatt
     9301 cttcaacatt tctctaaaat gtgtgcccga cgtacgatca aaaaaataat atccacacgt
     9361 tgccactacc gcacgtgtat aaaaactatt gttcaattca tgtctcatat ccgacaacca
     9421 acgattgtgc cacactacaa ttttcggtac agaagccaac ttcttaccac ctagtaggag
     9481 tcgatacacc aactctccct cttctccagc caatgccatc ctccctggac caagccatgg
     9541 acaaaaacct ccaatttctt gcacatcatg cttacggaaa gccatgttat tgcccatacc
     9601 caatgaaata tagtgaacga ttctagtatc atcaaacacc tttgttgctg gcgacaaaaa
     9661 taccgccggg caatatcttc ccacgttagc ttcaggctta taaggcaaaa catttccaaa
     9721 tacccccgca acatctttat ttttctccat tgccatagtt atatttttca accagctctt
     9781 agacacaata caatcatcat cagtaaacgc caatatctct ccctttgctc tctcaatacc
     9841 catatttaag gcaatcgttt ttccaaccac cttcgacttt atataaacta ctctcttact
     9901 tcccaaactt ttcaaaagat tccttggacg attatcttca ctctgatcaa gcaccaatac
     9961 ctcatattcc tgatcataat tatttttcaa tatcgagtct atacatctca ccaattgatc
    10021 cgctctatcc tttgtagcta ttaacaccga tattttcatt ttgaaatatg tcctaaaaat
    10081 aatttcttca tcccctcttt agaggagtat accccagcca acatcgacag ataacacacc
    10141 caagccgcca attcataacc tctagccaca actttatctt ttaacaaatt atcaatcagt
    10201 ttttttaaat aaaataagac caagacaggg aaaaagatcc atttaaagat atttcttccc
    10261 ggaaatagac ctaaagcctc aaactctgcc ggatgtctct tctccagatt cacaaaatta
    10321 cgaccataac tataatatct ttttacaaag tttatccaat tttgggtctc aaaatcatga
    10381 tacgccatta actctggatt atattccacc tctccacctt tataataaat ctttaagaaa
    10441 aaatctgtat cctctccggt tttcaaacta ttatcaaatc taatcttcca tttttttatc
    10501 ttttgtaagt ttaagactac cgccaaggta gcataaaact taactttttt aaccttttta
    10561 gatccagtta gaccttgaga aaataaccta gccgaatagt cagacagata accattttta
    10621 agaggtttaa cctcccccac cacacctact aactcattac ctctctcact tactgcctta
    10681 gacaccaatt ctatatagtt aagatctatc gctacatcat tgtctaagaa aaccatccaa
    10741 ttattatcac aagccctcat tgccaggtta cgagaataag caatattctt aattttagtc
    10801 ccaatatatt ttatccgtat cgaatcaccc aaaatatttt ctaatatttt tttagagaac
    10861 agtgccttta tgtccacaca ctccaccaaa actatttggt caggtttttc cgtctgccca
    10921 gccaatgatt taagcaatct taatgttgac tcttctctcc ccagagctgg tattgccaca
    10981 gatatctttg tcaacttttt ttattctttt gatatttaac caacaccgac aaccctccat
    11041 cacccacaat cttcccattt tctatccaaa ctatcctctg acagtgcttt tctaaataat
    11101 ccatccagtg cgataccaaa attatcgttt tcccttttgc aaaaaactct gcaatcttat
    11161 ccctactctt ctgttgaaaa tcccagtctc ctacagccac accctcatct agaaccaata
    11221 catccggatc tgcggccacc gctatcgaaa atcccaacct caatttcatc cccgaagaat
    11281 aggtataaag tggagcatta ataaaatccc ctatatcagc aaaatcaata atctggtcta
    11341 tcttcctaga cacctctttt ctggacatcc ccagcagtag accgttctga tatatattct
    11401 cccaaccgtt aagttctgga tgaaatccag cactcaaatc aatcaaagac accacctttc
    11461 cccaagttct aactgtccct ttgtttggag tggtaatacc agttattatt tttaaaattg
    11521 tcgtctttcc tgaaccattt gacccaacta tcccaatttt ttcacccttt ttaatatcca
    11581 aactaatatc atctaaagct ataaaactac tagtcacttt tcttctcaat agcagttcaa
    11641 ccaaagtcgg cttatcatag tgccaagcat attttttagt cacccctttc aaactaactg
    11701 ccagtttatt tttctttctc atatccaatc atcaaaaaac aaactttctc ttttaaaaac
    11761 cacaattccc aatgtcaaaa taaatatagt caccaacaac ccttgccaat aaaatccacc
    11821 agattctagg cccacagaca accttagtaa ctccaccaca cctgtcatcg ggttaatctt
    11881 taaccaatct gtcagccaca tcggcaacca ctctaaacta taaacaatcg gcgttgcata
    11941 aaaccatagt ggcaacaagg cctgaacacc aaaattaata tcccgccatc tcacatttaa
    12001 gctagacaaa agcaaagaca atccactagt caacatcgtc aaccagataa cagccattat
    12061 catcacccac catagctgcc agtatatctc ccctcttatt aaagcaagcc aaacaacaaa
    12121 caacactaaa gcaacaaaaa aatgaaaaag atttgacaac accactgcca gcaccaccac
    12181 ttcccgagga aattttgctt tttgtatcaa cgccctctca ttaacataca tcggagtatt
    12241 cttagaaaca gtgtaagcaa aaaaattcca agccaacaat ccaggaaaaa taaattcaaa
    12301 ataattatcc atttctatag gcacaaaaaa ctgaaaaata aatcccatca ccaacatttg
    12361 caatacaggg ttcaaaaaca tccacaaaaa accaaatatc gccatcttat atctagccct
    12421 aagctctttg agagtcatgg ccatcaatag ttcaaaccat cttttatatt tctttgtctt
    12481 catgattcaa tattctgatt ataatcgcaa atcctcatcc caccaagcaa ttggaaccac
    12541 tccccaacca aaaaaatctt tcttagcctc aggataacta ccaaaaacta gccgaccatc
    12601 actcaagttc ggagaatagt actcataaag tgtcccctca ctttcccaca tctctctaaa
    12661 ataatttaga tatttatttt ttatatcaaa actttcctgc tcataaccat tttcctttag
    12721 cgacaaataa ccaatccata tcaatggagg ccacacacca cctctccaat aaccaccttt
    12781 ttcatcaaat aaatccatat ctctggctat cgacaacata cctgttcctc cagcaaattt
    12841 atcactactg agtaaatatt tttccaccaa tactttcgtt tgtttttcat ctaatacttg
    12901 agcatataat gtccaaaaaa acgctgccga ttgtattgat ttatcaactc cgccgctctc
    12961 tccaatatca tagacaaagc catcttcctc actccagtaa ttatttttta tttcattagc
    13021 taaatcaatt tttcttccct caaattcttt agccacctca tcattattta atattttcat
    13081 tatctttgcc atgtctcctg ccatcgctgc ctgttgagaa attaaatcaa cataaccaca
    13141 attcgggctc tcatcacatc ttggtatatt gtccatcccc gacccccaac cagaccataa
    13201 atattttttt acctcaaccc catcgacatc cacagtcaca cctcttttcc tctctagcca
    13261 atcaaagtag ttaactaatt ttggcgccac tctcgacaaa cgaaccagat ctttctccct
    13321 ctcccatatc tctaattcta ttcgcgacaa aaacattggg ccactgactc tcaaatcatc
    13381 atagtcccaa aaagaaccta attctttatt gacttcgcga ggaatcaaac ccgcatgttc
    13441 accctctatt acttgtctgt cgtaccaaaa atctagggtt aatttagata ttttcgggtc
    13501 tgtcttaaac atccaccagg agataaaggc atcatcccac aaaaaactat tagggttaaa
    13561 agcttcatca aacttcacat aaaaatattc attttccaac ggcatcaaca tagttctatt
    13621 accacaatct actactacct tataactatt ccaaaaatct ctcttaacac tttttttttg
    13681 ctcaccctca catccatcaa tttgtttttt aactacaaaa tctatattct ctagagtttt
    13741 aagacgtatc tcccttacca tcttgtcacc aattccatat gaataacgcc aatgttcgca
    13801 gtatttttca ccacaacctt ttgtgtatgt taatccccat cccccaatga cgactaacac
    13861 tatcacaaaa taccagtcat taaatctttt tttaagtatt agaaattata tatcagtaat
    13921 ggccgaaagc accttatctg ctttaacacc gatcgtatcc aagtaccact gtattcctgc
    13981 ataccttggt tgattttctc tcataactat ttttaaagcc tccgacctcg acatcactcc
    14041 ctcccttatc tgattgcttc taaacgaatc attttcagta aaaccctgaa gttgacaata
    14101 gagatagtta taaaaagccg ggctaccatc atcaatccgc caggtcattc ctgattctgg
    14161 gtcaggcatc cacccatatt ccttggtcaa agtttctata atctcttttt catcccactt
    14221 aatataatgc caaagataaa cataattatg aggcactatg taagtcacca ggtaacccag
    14281 aaaagagctc cataacgatc tattccaata acccgagttg cccaaaaact gaaccaaata
    14341 atacttaagc aatcgccact tatctaccca agaataatca tgaatcactc cattcggatc
    14401 agcatttttt actccacaat aaccagcctt gaattcttcc cgctctaggc tatttcccct
    14461 acaaaaaaat accagtttca cccccagttc ctttctgatc ttatctacaa aatactccgt
    14521 cgttttatca ccttgcatca ataatggcac catccccaaa tcaggctttt ttaaccaagc
    14581 cagcaaattt tttcgtacat cttccctcgc cttctttata tccgccgcca ccactatttg
    14641 ttccacaccc aattttccca acattattgc ttgatttttt cttcccaaat cagacatcat
    14701 cccccaatca aaagttaccg ccaccggatg cattcctagt ttcttaacca agtaatgcaa
    14761 tccatacgaa ctatcccttc ccccactaaa ggcaataata caatccggtt ttccatcttt
    14821 tgatctatgt gcttttacca actgctttaa agaaccttca ccggctattt ccatcttttt
    14881 ataattacgg caaaaattac atactccctt attatcaaaa ctaatttgag gaacagtctc
    14941 cggcaaaata catttagaac accttttaat tttacctatc ctcttaaaat ctatcttgta
    15001 tttttcaatt tcttttctag ccagcaccaa tctcgacttt ggggcaactc ttctacccac
    15061 ttttaattcc atatcttgac gtaccaccac ccctttatca attttcagac aaattatttt
    15121 atctttaggg cactgctctg attgatcaaa aagtcttgcc tcagaagcaa attggatacg
    15181 accatcaatc tcaccaagat acaaagaccc tgtgtttgat gccaatatga gctgatcttg
    15241 ataatacatc gccacgctat aactacctac aatctctcgc aatattttag atactgccac
    15301 tacaaaacct cttttttcta tcaagcttcg tacaactttc accaaagtca aagaatccga
    15361 atctccgtca caccagttaa taccatattt atctgccagc tccctatcat taacaacaat
    15421 cccattatgt accaacacca acccatcact tactactggc tggttattat cataattctc
    15481 tttgccatta gtcgtcaatc tagtttgtcc caataagaac ttcgaactgt taataatctc
    15541 agatgaatct ttcaccaact tttgcactac atccgaatca cctctattag ggactttcat
    15601 caaaagttct gattcttggc cttttaccca aaatccagat gcatcttttc ctctcctaga
    15661 taatttgatc aaataaccct ccactacccc agatagatcg tcatcatcat tttttatcca
    15721 gccaaatatg ccacacatag ccatagtata ctcaattgtg gtcctgaaaa tacaaaaata
    15781 tatatctcca acctacatta tccttctctg tctttttttt tggatatatc tatggttcaa
    15841 tcgtaatcac tacactattc ccaaccctga tatattccaa tatatctacg aaggcagtca
    15901 ctatctccgc gggacttttc cgccaagcat tcacgctcca ccagcgagtc ctttctttat
    15961 caatctcctc gctacttttc ttcagtcctt tccagaacca gagctcttgt ctgcccacat
    16021 tctcaacatc acgactgcta ctgccaatct agccgtaata ttttttattc ttagacctct
    16081 ctcatcacta ctggcctacg ccgttccatt tcttctcgcc accaacaaaa tctattttct
    16141 caacagtgtc aatgttacca acgaagttat ctacagctta ttttttagtc tcgtcatcct
    16201 cttttatcaa cgcaaaaaat atcacttagc ctatctgatc gccggtatct cttttctcat
    16261 ccgttatgaa gctattaccc ttcttctcgg tctgatcatt gtcgatctgt attacaagaa
    16321 cccacttcac ctcatatttc gtcgatctct acttgctctt ataaccatac tcccttgggt
    16381 attcatactt aatgctcata gcactggtac caacataatt caaaatgcat atttagtaga
    16441 aatatataac ggtctaacaa atctccctaa ttggcgccct ttcatcactc tcaacgaact
    16501 aatcatcatc aacccaaact acaattttga ttttggtcca acgtttcttt atgtattttt
    16561 atttactatc tcattttacc tattaaaaag taccactcaa ccatcagtcc gactggctta
    16621 tattttagcg cttctccacc taacctttct tattatcttt ccaaactttg ccctccgata
    16681 cctacttccc ataatctggg ttatttattt agttgccatc aaccactatt accgcccact
    16741 taaatatttt tttctcggtc tgacactaac tctcaatttc tattacctac ctcaaagctt
    16801 acttaatgac acaaaacatc aaggttctga atatcgactc gttgccaact ggctcaatca
    16861 acaagacttc cccagcaaca tacgcatact tatttacgaa ccatggatta tcgactactt
    16921 cacctctaat ccccaagtag attttatcta cgatcaacaa tcatcttaca atctcaccca
    16981 acatattaac aactgcaaac ggaccatgac ttgtatagct gacaaactag ctcaagattt
    17041 ctctgactct agtattcttg tcatcaccac tgctacttct aacaaaactc tctcatttca
    17101 agacgaccat ttcaccgcca acctccactc tatcgacatc ttccaaaact ttcccaactc
    17161 agtcgactct caggaacatt tcggactaat caccacactt gacgatggac aaaactgggc
    17221 caaaatatat ttatattttc cttaattcta ttcagccaac ttacgtacca gcttctccat
    17281 tttttcttca aacttcttca ttttgaaatt attctccacc gtcacatagg tcaagatcat
    17341 aaaagcaacc actattacaa catccaccac ctctgatcca aacaaattat tcacttgctc
    17401 acgcaacacc caagggaaca ccaccacccc agcaaacgcc aaccatgtca tcaaccacat
    17461 accaaacatc aatgatgtaa tatcttgtct tttccaatgt aaatacaata tatagaccat
    17521 aaacaaagta aaacccaaca acgttatttg aattagatgt atattcattt ttcaaaaaac
    17581 cacaccggtg tctgacctag aatttttatt gcatcaacta cagacactcc cttatattta
    17641 tccaaataaa ttgtatcaat ttttatctct ttaaaaggaa tcttatacct acccacctta
    17701 gttgctatct cagtttccaa gccataacga cgtgaatacc aatagatttg tttatatatc
    17761 tcctttctaa atcctataaa gcccgacaac agatctttcc gacgaactcc ccacaaccat
    17821 ccaactaaat tggtagccac aatattcccc aatttcctaa tataaggtac ttctccatca
    17881 aattctcgat aaccaaaaac aatcaagtat ctttttaaag ccttctcaaa ccgcggcaaa
    17941 tctttaggac tatgttgtcc atcagcatca attatcacta ccgcctccgc ccctagtatc
    18001 caggcctttt ctaccccagt cttcaccgac gccccttttc ccaaattaaa taaatgatta
    18061 agcagagtta ccctcttatt ctttgccaaa aatttatcca acacctgcct tgtatcgtct
    18121 gtcgacccat cattcaccac tacaatttgg caaccagtcg actctaatat tttttttatt
    18181 acttcaacta cccgtgactc ttcgttataa acaggaacaa ccactattat tttcatctaa
    18241 atatatagta cagtaaggta tgcattcaaa taaaaagaaa attctatcca ccctttttat
    18301 ccttcttgtc ttacctttaa ttatttttgc cgccaaacaa gttatcgaca ttcgtaaagg
    18361 cgcctctgga gtccccgcca acattatcgt cgatgccaaa acagttcaag ggaatgttcc
    18421 atcctcactt tggcgaagtt tgtctcaagg tggagaagaa ccaaaagaca tgattggacc
    18481 tgtgctcagt caagtcaaag ctctctctc
//
LOCUS       DDWP01000013           14664 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_17316, whole genome shotgun sequence.
ACCESSION   DDWP01000013 DDWP01000000
VERSION     DDWP01000013.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 14664)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14664)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14664
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_17316"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3894 a         4082 c         2018 g         4670 t
ORIGIN      
        1 ggtcttgaaa acctccaaat cggttggccc acactttaat tttataaaat ttcctgatac
       61 aataaaccaa ttagttaata atttatccaa acttacaata aaatgatcaa gaagttatcc
      121 gatctagtca aatatgcagc tgttgctgct ccagtttatg ctcaagacat aaaattaacc
      181 cccaatgatt ccaattggaa caaccttacc aaattaacct ttggtggcat tatttctggt
      241 gctatcagct tagttatgtt ggttgtagcc ctagtcttct tctttatcct tatctacgga
      301 ggtcttaagt gggtcatgtc cgaaggtgat caaaaagctg tcgaagctgc tcgttcccag
      361 attaccaatg cccttatcgg tttggctatc gtcttcgccg cctttgctat tatgaagctc
      421 atcgaaacca tctttggtat tcagcttctt tctggattca aggttcctac cttccaatag
      481 tcttttgttc tttcgtaagg gcgaccctag tggtcgccct ttttggtttt atagtataat
      541 tcctctatga tctctcgtct aaaatcatca ttttcttttt tcttcttact tcttacttca
      601 tacttcgcag ttccctctcc tgtccatgct caatccgctt ggtcaggtcg ctgtgttgat
      661 aatggtgttg ccactattca aggtctagag tgtctttttg ccaacatcct ccaggttatt
      721 gttactctgg ctggttttgc tttcttggtt atgttcgttc tcggaggctt tcaatatctc
      781 accagcagtg gtgacgacaa gaaagctgct gctgcctcgt ctaccctcac tatggctgtc
      841 gtcggactag ttggtatcac tctctcttgg ctaattctga aatttatttc tgactttact
      901 ggcgccaacg tcctagaatt taaaatcccc ggcccctaat gcaatttaaa aaaattctta
      961 cttttgtttt gagttttatg ttttgcctgc ccgccgtggt gggtattttg agttttgctg
     1021 ctccagcagc cgccctcgat ccaattatta tcaaaaaagc cacctactcc gctccaccac
     1081 cccaacaatc tccccgaaaa gacgtcaact tctacagtaa cgacattggc aactacagtg
     1141 tcagttgtca tttacctctc actatttccc agtattttga cccagattgt gacaagatca
     1201 ctgggatctg tcgccgccaa gaaatcaaaa ggatagagca gcgagtttat ttcagcctca
     1261 acaattacgg taacttctgg tatcgcgaca cctacaatca ttttgccaat cgccccaaat
     1321 atatagacga tattaatttc cccaaggcca tgaccacctt tgatgttgat tccccttatg
     1381 gtgctaccaa tgcccacact cgatccaccc cagcctccct gctaaactgt caaaaaggtc
     1441 aacgtctagt tcaggccatt tattcactaa aagacaaaga cagcgaaagt tatgcctctg
     1501 tcgtcaatga ggctgtcact gaagttaacg gagcccccgt ccgcctagtt gatgttgcca
     1561 tcgctcttgc ccagtcacct tccctcaacc ccaacacttg ccttcccgat actgacaaca
     1621 gatccctatt ttacgatccc agtattagct gttccacctt ccccgaacct atccccttcc
     1681 ctggtgttcc cggtagttat aagggcacct ccatctctcc tgccgaagcg tgtctccagt
     1741 ttcaaagaaa cattcaagtc actgacattg gttctaatgt ccggcttgtc cgtgtcgacg
     1801 acgatgaaga aaatgccgat tacgatggtg acggtgttat cgactttgct aaaaaagcct
     1861 atacctccat tcctctgggc aatttaggaa caaacaatga aattccccgc ttattaattg
     1921 gcgcgggcca agtggtcgaa ccgcttgata tttgccaggc catcacccaa gtcgagtccc
     1981 ccgacaaagc caatagaatc ttcctcgatc tagttggtat caatgaagac ccctattttg
     2041 gcattgtcac cgacgaaggt aaaactttca aaaggacttt tactcgtact ttccgtattg
     2101 acgttcgtgt cccagagact tttgctacct acaaagtcgc ccttaataac cttctcccta
     2161 gtgagctcca aaaagaatac aacaccatca actccccccc atcagccatc gatcgacaga
     2221 ccaccctcga cccaggtaac aatatcgtcc actatgtttt ccgccaattc ctctggccta
     2281 gtaattaaaa ataaaatgtt aaatggttca atgttacaat aagtctatgg attttcgatc
     2341 tattttagcc aagttaaact atcgtgatac tcgcctgctc cccaccttct ccaccattgt
     2401 ttttatctcc attatttccc taatatttct ttcatcattc tccctcatca atcgttcatt
     2461 tgctgctacc cagaaaaata tcattaaaga acagcaagaa gcctgtggtg ccggcaacaa
     2521 tctttgttat ttcatcaaag ccgccaacga atcccacgcc tacaacgcta cttatgccat
     2581 gtttggtgac atccctactg atcagatcag tagtggaaat ggtttttgga tacccggtgg
     2641 tatcgttggt ggttccagca aagttatcgg ccagttctac aatcctgcaa tttccggcac
     2701 agaatatctt gccagtctca aaaatgaatt tttaggcatc aaaccagcct acgcccaagg
     2761 cgttggattt tatggtttac aaccaattct tcctgtatgg aaaagttttc gtgatgctac
     2821 ctacatgctt tcatctctat tcttcgtcat tatcggtatt atgattgtcc tccgcgtcaa
     2881 gatctctcct caagctacca ttaccatcca aaacgccatt ccccagctta ttaccactct
     2941 catccttatc accttttcct atgccattgc cggactcctt atcgacgcca gctacctcct
     3001 tcaaggcatg gctgtcagtc ttattgtccc caacagtgta gctggaggtg cccttggaat
     3061 ctttaacgga ttgattgatt ttgctggcca aataccacta attggacaat ttgtcccctc
     3121 ttttgcaaac ctcgttgaac caaccatcgg caccaccctc ggagccctat ctatacccac
     3181 acttgccact ctttatctag gtattatcgc tggattttct ctaggggcca cccttggcat
     3241 aattgccggt ccagccggta gcatcattgt cggagttggt ttcggcagca tagccgctat
     3301 tatcattctt atcatcttag gtatcggcat catcatctgg ctttttaaat ttatgatggg
     3361 tcttttaaag tgttacgcca cccttatttt caagataatc ctcgctcctt ttgagattgg
     3421 tatgggagcc ttccctaaca gtaaaattgg tttcagtacc tgggtaatgg acgttatcgc
     3481 caaccttgcc gtctttccca tatctttttt gtttattctt ctagcaagca gaataattgg
     3541 tttaattatc actggtcagg ccatatttac cattactgaa atggccaaac tccttaacac
     3601 tggaggtgca ggcggcaact ggcccgccaa caccggctta tgggttccca gcttacttga
     3661 aggtggcacg atgcttcctg gagcagtcat tgctgccgga gccattggcc tttccaccct
     3721 catgcttctt agcaaattac ccgaaatgat tccccaattt atctttatga tcaagccatc
     3781 tccatggggt caggctatcg gtgaaggtct caatcttaag aatcctgcca tcggtttcgg
     3841 tgctcgtgtt ggcaccaaca tggcagccaa tgctgctgtc gacaattaca atgaaaaaca
     3901 agccggtggt ggtaaacaaa caatgcttac tagtcttatc aagccagtag ttcaagccgc
     3961 tgagaccgta aaccttgtta gtgagcgcca caagttcaac ccccctagtg acgactaatc
     4021 tccttaatta atcttttttt tcaattgtta ggatttttat gctttatgct ctaggagccg
     4081 tcactttaac ctcaccactt atcaatttat caaatctcgt cactgcctcc atatcaccgt
     4141 tctccaaagc cgtatttcgc cattttaata actgcctctg ctcttcagat gcaaacctta
     4201 ccgcctcaat ccgctgtagt tcatattgat tctcaaccct cgcccatttc tgcctatata
     4261 cctgctccac agtttgattt ctcaataatc ccttaatagt atcttgtgtc cgtccccaga
     4321 tacctgcatc agccctcaaa aactttttaa actttgccgg attaacaaat ggctgaagtt
     4381 ggaattgatc ataacggcgc tgaacgtaag acccatacga agcattactt attgccccct
     4441 tcaaatagat tccctcacta gtcaatacat ccagatcagg tccaaaagta tttatcatac
     4501 tcatcgccca gaattcagga gtttttacaa ttgtagatat tctcccaggc aacttacctt
     4561 ttaacgctat ttgcgacaaa agacccaccg aactccgcaa ctcactgacc aacgtcccag
     4621 cccctttcat ttgcgattta cccaccaata ctgtccaggt tctcacggct gcttctgtct
     4681 catctctaaa gtcagacata atatcattct catcatcatc aaatttaaag tcgactcctt
     4741 cgtacagcgc tttaccaatt gtcattgtct cttttttcag cttccccttt ttacccttct
     4801 ctttgccaac taccacctct acctcttttg tatcataaaa actatagatt aatggcgtat
     4861 caaagaacca catatcttta tctctaatct tctctctcaa cgttttctta ccatctaatg
     4921 gttttcctgt ttcctcttct atcgccaaca ccatctccac ccaagctgcc accccacctc
     4981 caaaccattc cgcggttatc aggttcccat tctcacctac catttccaca ttcttcgcca
     5041 tgttgaatag ttgaagtttc ttccttgcct ttgcctccgc atttaaggtt cttattttat
     5101 ccgatagttt acgttcattc ggtgccagta ctcgatctac atccgccagc gacgatccat
     5161 aagtggtata gaacatgttc caagagttag ccgctattaa cttgtccatc tctgttgctc
     5221 tggtcggttt ggctgcctcc agttcttttg cctcctcctt atccattcct gcaaatctcc
     5281 tctctcttgc cagttttgta gccaaggact ccatatagtt atccaaattt gctattttat
     5341 ccagcacgcc tttgtcaatc ccaataccac ctttaccagc tttgtacaca aatctctcta
     5401 ccccatcagc agtaatttca aagacaccca aatctctcaa catccacatc gatactcttt
     5461 taaaatcggc gttcattaac gagttcattt cttttcctga atataaatag ctaggattct
     5521 gccaacactt ttccaaatct ttattttgat aaaatttcat ccaagatgct ccgtcagtca
     5581 atcttcgtgt catgtcaaag aaacgctttc tctccacact cgcttgtttg taccactctg
     5641 gtctattctc aattctccat gggacttgtt ccatcttaat tgctgtttcc acaaagtcgg
     5701 gaaactctaa atcatacatt cccgcttcca tctctccagc accaggacca tcagctccac
     5761 cctcagctcc aaccggctga atttttctcc gcataccacc actatatctc tctgatttct
     5821 catgtcttaa agcatccgcc ctcaaatcaa gcaagtatct tctggccatt ttatactctc
     5881 cagattgttt tatatcaccc gactcatcca acctctccaa tttctctaat cgaccaccta
     5941 tctctcttga cccatagtta gctgccagtt cttcctctaa taaagccaac tgtctaatta
     6001 tatttttatc agtcaaaata ttttgtgaca ccgccactgc accccccaca agcacatcac
     6061 tcccaaagac actatcaaaa gcagacgcta ctttcgctgc atcattccaa tccaccttgt
     6121 gttgcaaatt aactgctgca tcacgaatta atgtactttc ccaatcttct cccaaaccta
     6181 cctgaacagc ggctacctct gcccctacac aagcgtaata tatttcagca taattatttc
     6241 tccttgcttc atctaaatcc acacccccat aattatcccc ttctaaagcc agtcttaatt
     6301 cattgacatt ctttgggcca tcaggtccaa tttgagtatt aaaccatgat cttatctcta
     6361 ctctagcagt tgccctggca gaaacatcat cgtccaacaa tcttccaacc aattcccaag
     6421 atcgctctct cctttcttca aacctaatcg ggtcaatatg taattctgtt ttagcttttg
     6481 tgttcgcttt tactgaatgt tctgcccgac tacgtattac atcaacacct ttctctgcca
     6541 tatgcccaaa ttataccatc caacccccta ttcaaccact tccaacttta tcctatatta
     6601 ctccaccatt ttttgaaatt ttgatttttg tgttttgtgt ttatactaat gtatgtcttc
     6661 tattgaaaac atcgaccatc atgtccgttc ctatcgcagc gctctcaaaa gtagcctaga
     6721 aattaccatc tcttctctca ccaactccca gctcaggatt gattcaattc tccatcctct
     6781 tggcaaagat ccaaaaaaca ttgacttttc cgcccttatc tattctattc ttcgtcttcc
     6841 tcccgaggtc gacaagaccc atctcgttct catgggtcaa aaccctcaag tttttaccga
     6901 cgccggtttt cccaatgtca cttcttggaa aaaaattact tctcgtgctc gccgccgtac
     6961 tcgatacttc aatcctcaca cccatattgg tgttagtttt attagctcca tttccgatgt
     7021 tgacgatatc gttaatctcc ttatcgccta ccaatccgaa tggaacaaat tccacgctct
     7081 tctcaaatcc aactacccta ctcttcaggc ttttcgtcaa gccatcaaat caggcaagat
     7141 catcaccgac ttaagtataa gccccaaaga ctggcaaagt tttctgctag cccttggtaa
     7201 aaattatcga ctccggctca aaagaatcta cctccgtccc caggacattc gtcttcgtct
     7261 tctagctgga tcttggatcg actataccaa aactgttcaa ttctggtgga aaaatatcaa
     7321 tctaagttat tcatctcaaa ccaacgaaca tctttcccaa aaacgtatct atctagttag
     7381 tagtaacctc cacagccttt ccaatctcat taccggccta ccccgtattt acaaatccac
     7441 cctcatttct tatctcaaaa aagaaaactc acctcttctt gaaacttggc agaaaattca
     7501 atctggcgac agcctcattc ccgctgacga ttttctcagt tttatcttca aaaactattc
     7561 ccaccttccc gaatttcaat cagatctaaa ctcttcttgc gcccgctcag gtataatctc
     7621 cattcccagt tccgattatc tcgatgttaa ttgtcagatc tttcccatat cttcactcgc
     7681 taaatgcaaa catctcgacc cccgtcttaa aatatctaaa gcaccagcgc tccagcgttc
     7741 tagcgcacta attctaaata tcgactatcc tctcggtttc actgcttatc acattctcaa
     7801 cgaaatactc gagaatgtcg gtaaagtttg tggattatat atcctcggta aagccgctgt
     7861 tctcaactct gaaatcggcg atgtcgagat tcccaaagtt gtttttgacg aacacactca
     7921 aaacagttat ctctttaaaa attgtttcaa cacttttttt ccttttccaa accgacaagg
     7981 ctcaattctc accaatcaaa aagccgtttc tgtcctcagt gcctttttag aaaacgaagc
     8041 tctcatgcaa aaatatatgg aaaacaactt aaccgtcgtc gagatggaag ctggtcccta
     8101 tctttcagcc gtcaccgaag ccacttatga ccaacaactc ccccgcaata ctatcgtcga
     8161 tcttaacagt gctccttttg atatcggtat catcaactat actagcgata ctccctattc
     8221 tcaggccaaa aatcttggtg ccggcagtct cgaactcaac ggcatcgagc cagtttatct
     8281 cggctctctc gccatactcc aaagaataat taatctagaa gaatccagtt tatgagcgat
     8341 taccttatca gctcactatt aattcttatt ctaattttat acaagtattc aacccgtcgc
     8401 aatcacacca ccaaagccgg tgccccttgg gttccattag aaccacacgt catcaatcga
     8461 atcttcgagt tagttcagat aaaaccaggt gatatttttt atgatttagg ttcaggtgat
     8521 ggtcggatgg tcattgccgc cgcctccctt ggggccaagg cctacggtat tgaaattgat
     8581 ccttggagag tttggtattc acgcttttgc atctttcttt ttggcctttc tggtaaagcc
     8641 catgttatca aaaaaaatat ttttgacgtt gatatttccg acgccgatgt tgttacctta
     8701 tatctacttc aagaaactaa caacttacta tttcccaagt taaaaaaaga attaaaacca
     8761 ggtactcgag ttctcagttc agccttcaat ttccccaaat ggaaacctgt cactatcgat
     8821 cccaccggcc ccatctacgg cccccttcat ctttatcaca ttaaataata actttatcct
     8881 gtcgtcaaaa gaaacccact aatatctttt acctgtctgc cttgacccaa actactgtta
     8941 ttagctaaca tatttttgaa tgttctctca tctcccactc tattcttaaa atctgttgcc
     9001 aaagccacca gcttgtcagc tccaatactt tccagcgtct gctcatcaca aaactccggg
     9061 tgacttccca tttccatata cgcaaaaagt tccgtcaata ctaaacctaa atatactctt
     9121 tgtacaccct tatctccaac ttcaatatct aaaaatctat catcttttaa accaggtttt
     9181 aatcgatcat gttcgatttt tacttgatca ttaccatatc gatgcatttc caaataatct
     9241 gctccgggac gagaatacaa agcttccaca aattctttga acatctgttt cacgtcatct
     9301 tgagccgata ctagattcat caccacagat ccaacttcag attgctgatt accatgtaaa
     9361 cggaaatacg ccgcatgatc caactcatga acctctccca agccaactat cgataaattt
     9421 cccttctcga cctcgtcata ggcacctcca aatatataaa gcttatccat ctcaggcaaa
     9481 taagccatat aatggtcttc ctgctctggc caacacacca catcaatctt cattccagga
     9541 gacaattctt gacctgggaa cattttattc tctctctttg ctataccacc agtcgccact
     9601 gacttggcca cacttgaatc aacattttcc aacaccccat ctttacccac agcacacttc
     9661 ctttatacca aatacaatca ttatatttta ttttaaacta tttctaaaca aacagtaaat
     9721 atcccgattt acttgtgtat tacctaactc atctacatac atcgttatcc tccgcgacgt
     9781 ctctaaagta aacggctgaa ctttgacctc cagagccgct tttactaatt taccgatatt
     9841 attttgtctt atataccacg aaactctttc atcactcata ttttatttaa taaataataa
     9901 tttcgtcagg gtcaacaatt ggagctacac aaaattccct aaaatcttca tcacttacct
     9961 ccaacggaac tgaatcattt aaacaactcc ccctatcaga taccgttatc gcaattcccg
    10021 aagaagaact tgccgaaaaa tgccaacgca gacatttgtc ctcctctcct gccaattttc
    10081 cctgatactt caaagtccac cgcgttgcca ttctcatacc aatcatgtcg gcaaacgcca
    10141 ttgtatcttc aaacttttct atttttggta attctcctgt gtattttgtc ataggaccct
    10201 tttataacaa cactatatct gtttgtcaat ctatggtata ctcattagta atactatgta
    10261 catacattat gcaacaaaat caaataatcc agctcaaagt tagctcaaaa tttaaaaaat
    10321 ctctccaaca attggccgag tctcgtggac tacccctttc cacttatatt aaattcagtc
    10381 tgatcgagca gctcaagtcc gacccccttt ggaaccagct tgaacaatct tctacccgcc
    10441 aaactcaggt tgaagaattc attgaacttc taaaaaaatg atcactgccg ttatcgacaa
    10501 ccaactatgg atcaagctct tactcgacaa atctccttca ttaggttcaa ttattaacca
    10561 actatctaca aaagagctcc gtatctacac ttctccaact atagaatccg atctcatcac
    10621 tcaactaaat tcaaaactca aaccaaaact cgctatatca tttgtttcca ttttttttcc
    10681 attattcgaa gtcacctctc cgtccgaaaa cagattaccc ttccccccac ctcttccatc
    10741 atatcttgat ctttgtcttc actcacaccc agattatctc gtccctctca attaccaaca
    10801 atttaaagcc ctcgatggtt atcagaaaat caacgttctc gaatccgaca actttctcac
    10861 cattctaagc taaagcacta cggctctaaa gcaccaacgc gcttttataa attctccaca
    10921 ctcacccctc tccccctaac tgttctcaac acaaaaggcg ataagcccaa acccactaat
    10981 tttaacctta acctctcgat aaatttattc attgcccaca gagaaaactt atcttcatca
    11041 tcccacaata ttttcgacac ctcttcaaag ttgaccaccg ttcccttttt actaatcaac
    11101 aaagtcaata tctcattttg ttttttggtc aatttactat caatcggttt tcccaacagc
    11161 tcaatcttct tgttatttat cttcaacgcc tcctcagtta ctatccccaa tttttttata
    11221 aaatttcttg attcaaccct atccttttcc acacccttct ctcgcaaaga cttcaatgtc
    11281 ggtgtatgtt ccccaaaaaa atctcttaat attgaattct taatcacaaa atcaaccatt
    11341 gcttccgatt cttcccacgc catacccaat tcccctaaat acggataaat catcgccgtc
    11401 aaaatcgcct ctgccaaatt cgaaacttga gcatcttgac gaagataaac tattaatttt
    11461 tcatctccct tagacaaata ttggtaagaa ctaattgttc catatctagt tatcctcact
    11521 tcaacatttt ttattttctc agccacttct ggcaccaaaa tatttaccac attccaaaaa
    11581 gctttctcta cccttcgcca ttcattttca aaatccgcat ctactttacc aacctcaatc
    11641 ttatttaaaa tctctgtctc caaaacatct ggtactttta tcggaaaatt aagactaact
    11701 ccttgtaaga tcttttctac atccgttatt ttttctagtc caatatcagg caacaccacc
    11761 tctcttattc caccacttct tatttctggc aacaccaaaa atcctttttt aatataaaaa
    11821 cccgaccatg tttgttttgc cgcatgaacc actcgctcta cttcagtttc tcgactacag
    11881 acaaatttag ctttaggtat gtccatatat tgtccagtat tatatctcaa aacacctata
    11941 ataacgagaa atgaatacag aactacgaaa ctattattct ctagtctacg aaattcatct
    12001 agctgtcagc agtggagacg atacagccag gatcggcgta tctggtatga tcacacgcaa
    12061 agaagaactc gccaaaaaat tggggcttga acaacatgaa gaacgcatct tcgattttgc
    12121 aactagaaac tcgtcctatc ggagatccta acttttcata aactctccaa actccacatc
    12181 gtccaaactc tctttctcta ctaggagttt tgccaaagca tcaagccttt tcttatcttt
    12241 cttcagcacc gtctcagctc tcttcaaagc cgtattaact agttctttaa tttcctgatc
    12301 gactttttcc tgcatccctt cagatattct cgctggctcc atccacgctt taccaaaatc
    12361 atccatatta atctgtggcc ccatgtttac tggaccaagt tccgacattc cccattccac
    12421 caccatcctt ctagcaatgt ttgtcacgtg ggaaatatca ctacttgctc cggtcgtcac
    12481 atctttaaat atcaaagctt ctgccgctcg accacccata gccatggcca tttgcttaat
    12541 caatcttgat ttcgtttcat gaactctgtc tttctctggt gggattaggg taaatcctag
    12601 cgccattcct cttgagacta tcgatactcg atgaaccggg tctaattctt ctacaaagtt
    12661 tactattgca tgtccagcct catgataagc cgtcatcaac cgatcatcat caccctgatc
    12721 tcttcttttt tccgatccca acttaacttt aagtgctgct tcttcaatat cacccatatt
    12781 aatctttgtc ttattctctc tagctgcctt tattgctgac tcattcaaca tattttccaa
    12841 gtcagccccc gagaacccga ctgttctctt ggctactgct ggccatctta catcaccatc
    12901 aaaaggttta cccttcgcat gtatctttat tatctcttca cgggccttta gatctggcat
    12961 ctccaacact actcttctgt caaatcttcc cggtcgcatt aatgccggat caagcaaatc
    13021 tcctcgatta gttgctgcca acaccaccac tgctgtatta gtttcaaatc catccatctc
    13081 cactaatatt tgattcaaag tctgttctct ctctccatgc cccccatcca ttccccctct
    13141 cactcgacca attgcatcta tttcatcgat aaaaataatt gccttacctg cctttttggc
    13201 agttccaaat aaatcgcgaa cgcgagacgc acctactcct accaacattt ccataaattc
    13261 tgatcctgcc attgagtaaa aaggtacgtt agcctctccc gccactgcct ttgccaacaa
    13321 agtttttcct acccctgccg gaccgactaa caatactcct cttggagctc ttgcccccat
    13381 ctttcgatac ttttctggat gtttaagaaa atccaccact tcttccaaat cttttttcgc
    13441 ctcttgtact cctgccacat ctttaaatac taaattctgt ttacctttaa taaacaattt
    13501 tgctgtcgat ttcccaaaac caaacattcc tccaccagcc ccaccggtct gtctaccaaa
    13561 aaaccaaata ataaaagcaa tgggcagtcc tacagttaat agcattccta ccatttctgc
    13621 caacattttc caaccagttc tgttttctac attaaaatta attttcgaca catctactcc
    13681 ttctctttgc aacacttcta ccatcgaagt tgatccctct ttagacgata ccaaggtctt
    13741 atcagtatct cgaatcttag ccaacacctc gttatccatt accaccacct cacttacttt
    13801 ctcacttttt atcgcttcaa caatttccga caaagacact ttctccacag tacctggtgt
    13861 taatatttga ctaactaccg aaaaaaacca gaatgccaat gctatataca agactgtctt
    13921 tataatctgc tttggcccaa tttcaattcg cttcactttt accccaggcg gcaaattatc
    13981 cgtgatttct tttgggatct tatctttatt ttgtttctta tcggatttta agttttgagt
    14041 ttttagtttt aagtttttct taatcttgtc cattaatgag tttttctttt ccatgggaat
    14101 cattatacca ttctgacacc accctattat ttccgcattt tccccgtttt attaccgcac
    14161 tagctccatc aacttctctc tatcatcccc ttttccttca cgcacgctaa atgtgtgccg
    14221 acactttgta cacttatgtt taattgtaaa ttgaaaattg gaaattgaaa attgcgtagc
    14281 tactggttcc atcaaccctc gacactccga agctctatct cccggtattt cctcatctac
    14341 atgcttcccc cacaaacact tcggacaatg atcagtatac ccatcaccaa tcacttcata
    14401 cccacaattc ccacaaacaa aattttcaac aactctttta aaatttttca tatttttact
    14461 aaggctctag agtactagcg ttctaacgca ctatttcgac ccccattcta atatccttct
    14521 ctagtttctg attaaataac aatttcccat tcaccaagct cgccatcggc tcagcttgat
    14581 tcacttgcca cccctcgggc actgacacca gagccaccaa cccagtatca cccgatcctg
    14641 attgtctttg tatataattc aagt
//
LOCUS       DDWP01000014           10420 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_8599, whole genome shotgun sequence.
ACCESSION   DDWP01000014 DDWP01000000
VERSION     DDWP01000014.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_8599"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3427 a         1388 c         2535 g         3070 t
ORIGIN      
        1 atactgtttc gtcagtaatg gcggccgcgg cagtatcggt ggttagatca agtatggaac
       61 gcaaagttga cagtatcgtc gtgtttacag aaactggggc aagtgcccgg acaatagctc
      121 gttatcgatt ggatgtacca gtaattgcag tgacagcaag tgagaaggtg gctgaagcta
      181 tggcggtgtc gtacggaatt acaccggtgg tcattaatat gaaaggtggc aagttcacta
      241 cacctaaagt ggtgtttgac gaattagtta aaagagaaat attagatagg ggtgatctag
      301 tggtcgtggt ccatggttca aaatggtatg gttttggcat gaccaatgct gtccaggtga
      361 gtgaggttta aagtatcctt gacccgataa cgacactgct aatgccaaat agatgagaga
      421 ttaaggagac cagtaaaaaa cggggcatag tagtctggga aagtcctaag agactgatgc
      481 cgagttcgtc gggaagtgga gaggcgagta ctagggcgcc gagaactggc agtgtccagg
      541 caaagtattt ggtgtgaata atctttttta aatgactgtg atgctctatt tcttggtata
      601 tccgggaaac ttttgagcta acattatctt tgaaaagtaa aaacataagg gcgtcgcaag
      661 taacggtgcc taaaccggtg attaatatta ataaaattgg attaagagtg gtagctagat
      721 ggacaataat taagccacca gcaatagcgg taaaagtaga ggcaaataat gccccagcaa
      781 taaaggcaaa aacaaagggt aaattatcaa ctagttggct aaattgagag aaaattgggc
      841 tttgggtgaa agcgatgacg gcaataaagc tggcgataaa taaaaaaata tttctatgcc
      901 ggtgacggtg gtagcgggga tgcatggttt atagaataac aaaaaaatta aaatagttcg
      961 tattgccatt gaggtccata ttgaccatag cttacaaaaa gcatacggtc tggatattca
     1021 ggtaattgag tgaaatatct ttcaccattt gtttgggaat aattagaagg gcagatatac
     1081 ggatcctcga tattttgtcg ggtgatatct tttatttgaa cttttccgtc tttttcgatt
     1141 aatagacact tatcttttgg ataatttttt agaattatat tggtgatagc ctgatttaga
     1201 gttaaggtag ggtctttgaa aaaaacttgg atgaattggc cgttaatttt tccttgtgga
     1261 tcaatatcgt agtcaccgta gattttgttg tcgatttctt tgatttggtt aaggctgtaa
     1321 actctaactt tgagtttttc tgaggtgtag gtgttaacaa ctgatccatt gccccatttt
     1381 tttaattcgt tgattatcga tgagttgtaa ctgtattcat ctgctttagt atttccgtca
     1441 attggtgcgg ggttatcagt atttggatca tcatgaaatt tccaaaaact tttgttatca
     1501 atgttgattt ttaataaatt tcctggagta gaggtgtcaa cttcaaattg ggaaatagat
     1561 agagtagaaa tttttgaatc tgtttcgatc acatttaagg tgaagtcggt aagatcgtat
     1621 tctgtaccac atgatcctga ggatttgatc tttttaccgc caactctttc taaagtaagg
     1681 cctgcagtta acataagctt gtcgccctca attttccagg taccgtcgat ttgttctgtc
     1741 gttcgatcac atttcatttc gctggtgaca aaagaataaa agccgtcttc gtaaaaatga
     1801 tatcgatttg accagcctga acccattgct gatgtatctt gccagacacc tattagtaaa
     1861 ttattaggat tttctgtttt gtctttttga aataaattaa gagtgatacc ggtggtgatt
     1921 agaatgacaa aagaacttaa taataaaata aaaacgtttt tgttgttttt agcagatttt
     1981 ttcttctgtt tcataattta tattatcact accaattgag atataatgaa atatatggaa
     2041 acattaacta gttggatact taggtctttg gtaattttag caacagctta cctggtgcca
     2101 ggttttatcg tatcaggttt tactggagca ttagtgctgg tattggtact cagtttattt
     2161 aatattctaa ttaagccatt attgttttta ttgacgttgc caataaatat cctaacttta
     2221 gggttattta cttttataat caatgcgatt gtattgcaaa ttgccatgaa ggtagtacca
     2281 ggagtggaga gcgattcatt tacaacaact ttaattgctt cgattgtgat gagcgttttg
     2341 tcgatggttg ttggtgggtt ggtggggagg taaaacagaa tctcttgttt taatctcttc
     2401 tattaattgt tctaatgtat agattggcct tgtagcttgt tggataaagt ctttgactaa
     2461 aggtgcatta agaaattcgt ttattctgtt ttgcatagta tgttttagga ccaaagttta
     2521 aaaccgttgg tcaaactttt aacaagggag ttttcgccaa atataccagc accgaggtag
     2581 gtgaggtttt cttcactttc tctactaact aatttactga tttcatcatc atttgaggtt
     2641 tcaatcattt ccttaataaa gaacattgat tgaacgtcag tttgtctggc tttggaagca
     2701 aagctgtaga tttcggcttc agtagcggag aaagcgatga tgggatattg ggtgttgcga
     2761 ggaatattaa taccatcttt ggtgacgaaa tattctccag tatcaaagct agttcctaat
     2821 ttattaccaa aataagcaga gatatgggca acagtattta aggcttgcca agcaggtagt
     2881 tccttattga cgacgatgga aattctttta gaaaaatctt gagacatata attttaatta
     2941 atattttgag tacaggccga ttatatcgag ggaaaatgtg aatgtctaat ttttgaggta
     3001 tttacacgtt gttatacaag ttgtataata ttatacaaat tgtataatat tatacaaata
     3061 tgataaagat atcaacaatg gttcaagata ttatggagaa tgatgatttg gttaagagtc
     3121 tagcttcaaa agggttgttg aattatatgg cttatgccag aagtattcag aaagaggttg
     3181 aggaaaaggc gtttaaggat attaagttag gttcgattac agcggcggta actcgatacg
     3241 ctaataattt gaaaccgatt gatttgccgt cagaaagaga tattcagcaa atttctttgc
     3301 aaacaaattt ggaaggggtg acatatgaac ggtcggaaga agtgtcgaag aaaattcagg
     3361 cgatttataa tgagataaaa gttgacaata agacctatat cacgattacg caggggatta
     3421 atgaggtaac aattattgcg gaaaagtcgg taattgatat attcagacaa aaattaaaag
     3481 agtattcgac tatttatgat atttcggaaa tagtaggaat aacggtgaag tttggtttga
     3541 aatatatgtc gattccgaat ttattttatt tactgattcg taaattggct ctgaggaata
     3601 tcaatattgt agagatagtg tccactgcta ctgaactgac gtttattatc aataagaaaa
     3661 atctacaagt ggctttggat cagttgcaga aagggttgta ggagttgcgg agaggatagg
     3721 agtcgaacct acatgccatt gctggcgctt gttttcaaaa caagttcctt accattcgga
     3781 gcacctctcc aggtttaaat ggattataac aaaaatgaat agctattttg atttataata
     3841 gtgtaaaata tagagcaatt cgcccggata gctgtccgat gcgagctact gctcgatacg
     3901 gactggggat aaaccccaca gttgtttcta cattggatta actggttcga aaggctagaa
     3961 tttaacaatc cgcccggata gctcagttgg tagagcatcc ccttggtaag gggaaggtca
     4021 agaattcgaa tttctttccg ggctcagatg aatatgatat attggtaatt atggtagcga
     4081 gaagtctttt acataaagat cgaggtgtcc cgaatataaa accttctacc attgaagcaa
     4141 tactgacggg aaaagtaaaa atagtaccag ttgatggggg aagttattat caagaaaaag
     4201 agataggtgg gtatagatgg tatggaacat catatcagta cgacatagat ggtttaccaa
     4261 aaggtgttaa tggagaatct ggtacttgga atactcagct gattgtgtgg acaccaaatc
     4321 cattagtata ttctgatctt cgtggaagac caccaggtat gcagatttat attgcaacgg
     4381 gtgggggaga aatggttttg acggataacg aggagcacaa agtggctgtc tttcaagaaa
     4441 tatcgaggca attagaagaa agtgcagata aataatatct tcttgtgtaa atagttaact
     4501 ctggctaaaa tcgtgttcat gtcaaaatca cctgcacgaa gtggtgggcc taaagaaggt
     4561 tcaatactta ttcttagaaa acacccagat attaaaccgg tgttggctat aagaaatttg
     4621 aaacaatatc ctgatggtcg aataagtttt actttggcgt cggtaagtga gcttactcag
     4681 gctgagttag tagtggcgag agaagcggtc ttaccagaga gactggcgtg aactagttga
     4741 ctaattggga cttgtttaat tgactaactc cgactaaaat agtgggagta tgattaagat
     4801 aaacaattta catgtatctg tcgaaggaca ggaaatactt aagggtgttg atttggtcgt
     4861 ccgtccggga gaagtccacg cggtaatggg taagaacggt agtggaaaat cgacattagc
     4921 ttttacacta gcgggtcacc caaaatatga gataaaaagt ccaaagtcta aaagtctaaa
     4981 agtcgaaagt ggagcgattg agatagatgg aaagataatc gatgagttag ctccaaacga
     5041 gagagcggaa atgggattgt ttttggcaaa ccaatatccg gcagcagtgc ctgggttagg
     5101 atcgacaagt tttctatggc agatatataa gaaaaaaaac ccttcgacag gctcagggcg
     5161 agtcgtcacg agcattattg aatttagaaa atggattgag gaacaggcaa aggtattaga
     5221 tttgaatttg gaattattaa aaaggggatt gaatgacgga ttttctggtg gagagaagaa
     5281 aaagatggag atattgcaaa tgttagtgtc taaccctaaa tatgtggttt tagacgagat
     5341 agattctggc ttggatgtag aagctttaaa gaaaattgcc accactgtag ctaaagtagc
     5401 taaaggatta aacatcggag tgataattat tacccactat tccaggttgc ttaaatatat
     5461 caaagtggat agagtgcata ttatggaagc gggagtgatt aaaaagtctg gtgatagtag
     5521 cttgatcgaa aaaattgaaa atactggttt tggagataat gacaacagac tataaatatg
     5581 gtttctccat gccggagaac aactatgttt acaaatcagg tgaaggttta aatgaagaag
     5641 ttgttctaaa aatttctcag atgaagaatg agcctttgtg gatgacggct tttcgtctaa
     5701 aaggctatga attgtttttg aaaaaaccaa ttccagaatg gggggctgat ctatcgacta
     5761 ttgattttga taaagttcat tattttgtcc gagcttcgga taaaacagag aaagattgga
     5821 acgatgttcc ggcggaaatc aaagaaactt acgatcgctt gggtattcct gaagcagaga
     5881 aacaattgtt gggtggagtt aaagctcaat atgagtcgga agtggtctat ggaagtctat
     5941 taaaaagatt ttcggatatg ggaataattt tttgtggtat ggatgaggcg atagaaaaat
     6001 atcctgaatt agtaaaagaa tatattggaa aattaattcc gccaggggac aataaatttg
     6061 cggctttaaa cacagccgtt tggtcaggtg ggtcgtttat ctatgtacct aaaggggtaa
     6121 aagtagattt accacttcag gcttatttta ggataaatac agctaacatg gggcagtttg
     6181 aaaggacatt aataattgtc gatgaaggcg cgtttattca ctatgtagag ggttgtactg
     6241 cgccaattta ttcgaccgat tcactacatg cggcagtggt agaaattttt gtgaaaaaag
     6301 gggcaagatg ccgttatact acgattcaaa actggtcaaa aaatgtttat aacctagtaa
     6361 ccaagagagc aaaagtagaa gaagatggag tgatggagtg gattgacgga aacctaggaa
     6421 gtaagataac tatgaaatat ccggcagtag tattggcagg taagagagct catggagaga
     6481 tattgtcggt ggctgtagct ggtaaaggtc agcatttaga tgctggagcg aaatgcattc
     6541 acttggcgcc agggacaact tcaagagtaa tctctaaatc aattagtttt gatggaggaa
     6601 gggcctcgta tcgtggatta gtccaggtat ctcctggggc taaaaatgct agaagtaagg
     6661 tggtttgtga tgcattgata atggacaaag attctcgttc ggatacttat ccggttaata
     6721 aaatatttga aagtgagtcg acactagagc atgaagcgac ggtgagtaaa attggtgaag
     6781 agcaattgtt ttatttgatg agtcgaggat ttgagcagca tgaagctgag gcaatgatta
     6841 ttaatggttt tatcgaacca atatcaaaag agttaccgat ggaatattca gttgaactaa
     6901 atagattaat tagcatgcaa atggaaggga gtgtggggta aagaatatgg aaaacaatct
     6961 tattaaaaaa atacttgtaa aaaaaggtga aaatgtggtg tgggatgaga tgatggtgta
     7021 tgacaaagat aacttggaag gggaggtaga agtgcgggcg gtagtggagg ataatggtgt
     7081 cttgaaatta aaagggatga ttttgataaa agaaaatgcg gcaggggcga atgcattttt
     7141 aagatttaaa gtgttgttac tgggtaaaaa tgccagagct gaggtagatc caggattgga
     7201 gatattaact aatgatgtta aggcttcaca tgctgcgtcg gtagggcagg tggatgaaga
     7261 acagctattc tatttgatgt cacgtggact ttcgcgtaaa gaatcagtta agatgatagt
     7321 gaaagcgttc ttgagttaaa tcctaaatcc taaatcctaa aatctaaatt ctaaaaaaaa
     7381 tgaattataa aaaatatttt ccaatattta aaaaacaacc gaacttgcat tacctggata
     7441 gtggggcgac ggcattgaag ccacaggtgg taattgacaa aatggtggaa tattacaccg
     7501 attattcggc taatattcat cgcggtttat acccgatatc agagagggca acagaggagt
     7561 atgaactggc tcgagaaaag gtggcaaagt tcataaatgc caaaatggaa gaaataattt
     7621 ttaccagcgg gacgactgac agtattaatt tggtagcgag agcaatgggt ttgaaaaaag
     7681 atgatgaagt agtagtagaa attgaggcac atcattctaa ttttgtagtg tggcaacaat
     7741 tggggaaaat agtatttgaa ataactaaaa atacaaaaat tttggcgatt acacatgtgt
     7801 caaatgttct tggacaaata aatgatataa aaagtatcat tgagcaggca cgaaaaatta
     7861 atccagaaat cgtggtagtg gtggatgggg cccaagctgt ggctcatatg ccagtagatg
     7921 tgactgatct tgattgtgat ttttatgctt tttcgggaca taaaatgtat ggaccaacag
     7981 gagtaggagt gttgtataca aaagcaaaaa gacaattaga gatggaacca gctaagtttg
     8041 gtggagggat gattaaaact gtatctacta gtgaatcaac ttgggccaat ggtccggaaa
     8101 aatttgaagc ggggactccg ccgattgccg aagtgatagg attgggagaa gcaatagatt
     8161 ttattaacga aattgggttt gaagaaattc aaaaacatga gaaagaatta acggaatatc
     8221 tactggaaaa aataggtgaa ataaattgga taaaaatatt agggtcaaga gaaaataaat
     8281 tagggctggt ggcgatgtat tctgatgatc cgaaaatggg gacatcgcat gatattgcgg
     8341 atatattggg gagagatttc aatgtgtgtg ttcgggcggg acatcattgt gcgatgcctt
     8401 tacatacgaa gtttgggata gaaactggga ctgtgagggc gagtttgggg atttataata
     8461 caagagaaga tattgatgaa ttaataaaag gtttgagtaa gacatggatg atctttacaa
     8521 agaaataatt ttagaccatt atcaaaatcc taatcatcgg gggaaattga ttgatgggat
     8581 agaggctgga gaggtgaaca tgagttgtgg agatagtttg aagatctcgg taaaaattga
     8641 ggaggataaa attgttgatg caaaatttga tggtggaggt tgtgctattt cgatggcggc
     8701 ggtagatatg ttgcttgata aagtgattgg aatgaaattg acagatgtta aaaaaatgtc
     8761 gggggcagaa attgaagaga taatgggggt ggagttgacc ccaagtcgta aaaaatgtgc
     8821 ttatctggga ctagaggtat tgaagaaaat tgagatataa taaggcacgt tgcctgaata
     8881 gctcagttgg tagagcaagc gttttgtaaa cgctaggtcg tcagttcaag tctgacttca
     8941 ggctcaatgc tcaaaaaaaa gatattggtg ctgactgatg atatgccgtg ggggcatagg
     9001 tcgattgcta gggcaatata caaatactta aaaggtgtcg agaaagaaaa taactttgag
     9061 gtgggtttgg cggaaataaa atcacctttt ggggtgatga atgaccttta tacttttgta
     9121 tatagatttt tcccgatatc taatcgaata agtatgaagt tgatggagaa tgggatgtta
     9181 cgtaaagctg cgtttgagat gttggactat gatatacctg agttggaaaa gacagtaaat
     9241 aagtttaaac ctgatctgat aatttcggct tatttttatc actctcaatc gttggtaaaa
     9301 atgagacagg ataaaaaatc acaatttaaa ctttggacgg tagtcgctga tccatggtca
     9361 ataaatgctt tttcatttat tgaaggagct gatttgcatt tggtatatga ccagatgatg
     9421 acaaaagcga tgaaggaata tggtatctct aagaataagg tgtttgaaac tggttggtgg
     9481 gtcagaccgg aaatgaacga agataaactc aaaactcaaa actcaaaact caaaataagg
     9541 cagaagttgg gatttgagga tgatagaccg gtggtgtttg tgggtggggg aagcttgggg
     9601 acaaattcat tggtgaaatt attgccggtg ttaatgatgg ttaagactaa ggtaggggta
     9661 atatttaaca ccgggacaga caagttggct tacaatatgg tggaggaata tataaaattg
     9721 tttaagaaac tgaagaggaa tgatttggtg caaataaaga atatgggatg gattgaaaat
     9781 atgggtgagg tgctatcggc ttgtgatatt gtctttggta aggcgggtcc gaactttttg
     9841 tttgatgtgg tggcggtagg taagccattt gtggctataa ctcatatcgg cgggcaagaa
     9901 gatggcaata ttgacataat taaaaagaag aaattgggtt gggttaaaga agggggaaat
     9961 tcttcggcaa aatttttact agaatatcta aagaacccaa aaaaatatca ggaaaaattt
    10021 aaagagacta ttaagaaaga aggagagaga aatgaaaaga gtttgccaaa gatactagag
    10081 aggataaaaa aagaattatc ccacccatga catacttcaa tattggcttt tccacgctta
    10141 aaaacgcggt gggaaactgg cctgtacaag cgaacccgca caaacacgtg catcccattt
    10201 tagaagatta gctagaaaaa acaccttctc aaagatagtc ctgggtagga tgttaaaatg
    10261 ataccatgat tacgattcca aagaaagttg aatacagtat aattttgatt gcttttttgg
    10321 caaaaaatga tggtaaaaca gtttctttgg cggaagcttc gaagaaatta atgttgccat
    10381 ataggtttat gggacaattg gcaatggctt tgaaagtggc
//
LOCUS       DDWP01000015           11427 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_18856, whole genome shotgun sequence.
ACCESSION   DDWP01000015 DDWP01000000
VERSION     DDWP01000015.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_18856"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3429 a         2826 c         1906 g         3266 t
ORIGIN      
        1 cttaccttag ttccattacc aattactaaa gtgtctccat caatcacttc tcgcaccaca
       61 ttccactgcc aagtagacaa caatcctcct accatcaccg ctcccaccac caacgccgac
      121 aattttgttt tcttcactat tttttagtat aaagtatttt tccaatttta taaaaaatat
      181 ttagcaaaca cctaagttta tgctaaattg ttattgtgca actcttcatc tttgaaacca
      241 tttttgtcgt tttcttaccc atttctctaa tgatactttc tccccaagta tacgtctttc
      301 gccatctaat aggcatcgcc attctagcct atttttttct catgcgacgt catttttctc
      361 tacctcctct cgtcacccag cttaccatcc ctccgctaaa acactggatt gaaacagccg
      421 tcattactct aattcttagc ctatctctta tcatcatcaa tcactactat cccgagtcag
      481 ttcgtcagat ggtgcaatca cttttctctc atgacaaaaa gcttctagtc ttaatttcct
      541 actcactttt atcagtacct ctccaagaaa taatttttcg ttggctatat cttcgtcgtc
      601 ttcaactttt gagactccac ccagcttttc caattatctg gtcatcaatt gtttttagtt
      661 ctgtccattt tccttttcat agtccactaa tgattatcgg aaccttcatc ttaggactat
      721 ggtggtcata ttccactatc aagcaacagt ccattattcc agtctccatt agccacgccg
      781 tcttaggggc actaattctc ttgctagccc ttttctaaag tatgaatatc actaccatcc
      841 ctacaattaa agacttttac cagcaattgg accagtcttt ttcacaagcc aaaaaccaaa
      901 ttcttattaa cactctagaa ctaagtggcc gacacattta taaaactatc ggatcaaccc
      961 taattaccaa actaaaccaa ggctgccaag ttaatgtttg tgtcgatctt gttggcaata
     1021 cattagcaca aattcataac catcgcttat tttctacacc catcatttca attctaaaat
     1081 caaaaggtgc caccaccaat tattatcgtt caggcaaccc tatttttcac aaccacgcca
     1141 agatatatat tatcgaccaa cagacaatct atcttggtgg ctctaatctt tcagaccatt
     1201 ttttaccttg gaccgatacc aacttcaaaa ttgtcctctc aaagccaaat cccgatctgg
     1261 tccattttta tacccaaatc aatcaatcgt ctcaaaattc ttttcaaacc atttatttag
     1321 atcaagaaaa cactctccat tgtcacaaac aagaccgagt atttgaacag gaactattaa
     1381 aacttatcaa ttcatcccaa aaatatcttc gactagttac ttggatgctt ttgctttctt
     1441 cagaaatatc cgaagctatc atcaaagcca agcagagggg agtcgatgtt cagctagttt
     1501 attcccaaag caatttcatc tctccactta gtcttatcaa ttttccagga atcaaccatc
     1561 tcaaagccca tggtattagt gttaagaaaa gcattcctcg atacaatcac agcaaactct
     1621 actggaatga acaccaggca attatcggat ccaccaatat tgattggccg gaattcattg
     1681 taaataccga gatcgtcctt gtctccacct ctaccaaact agttaaagat ctaaatcatc
     1741 tttttaccca attttaatac ccaactacca ttccacgctg tcgactaatc cttggaattc
     1801 cgactcccat tctttgtcag ccgaatcagc cgttaaagtt atagttagca gccttgctcc
     1861 ttttttcaaa aatatcgttc tttctttttt atccgcagtt ctaaataaaa gccctctttc
     1921 atctcctttt attccgactt cttcactata ttgtaaagta ttccttcttc tcatctgaac
     1981 ttcagtcagt tcagacagat cttggctagc tccacgcaaa ttaaacaata ccgtacttct
     2041 ttccccagtc aactttagat taaccaacac tctcccgtca gtggttaata tatcttcgat
     2101 aatatattca ggacgatatt taaaacttat gtaattactt ctaaacaccc cggggatact
     2161 cttactttgt tccacatcat taacaacatc tttatcttga ccattaccta ctaacatcgc
     2221 caagactatc aacactgcca gcaccccacc cactatccac aaatatttta atttcataga
     2281 taaattataa taccttatgc gcaccgacaa acctacttca attgccgaca cactcttcaa
     2341 actatctgac actctcgcca agcggaaaaa atatgtcaaa aatgaatttc aagcctatgg
     2401 gctcatgtta gccgaagagc tagacgactg gaaaaacaaa tctctatata tccgtcttgc
     2461 caaaagcacc gatcgtcagc ttctagaacg ggctcgctat tttgttaagg accacaatcc
     2521 cggccaagtc aaatctctcg gtgctctatt catgtggaaa atcaaagaat taaaacgcga
     2581 gagtttgata taatactcaa atgaccaggc tctcgagtat tcttgtattt ttggcgctct
     2641 ttttaggcgt agttttacta ttaccccaaa ttcaagctgc caccaccaaa ggtgccaccc
     2701 taaaatacga tgctaagaaa accactctct atactccacg aactgagtcc attgccgaca
     2761 cactcactct caccggttct atagagaccg atcaaattgc ctctctccgt tttcaaaact
     2821 ccggcaaact tgtttgggta ggtgtcaaag taggggatag agtaaaaaaa ggtcaggcca
     2881 tcgctagtct cgacaaagcc gaacttcgca aaaatctagc cacccagttc aacaactatc
     2941 gcaaatcact cagcgagttc aacgacaccc aagacaaata caaaccagaa aaagacgcac
     3001 tactcgtcac cgacaccatt cagagaatac tcgacaggac tcaatattct cttgacaatt
     3061 cagtcattaa ttacgagctt accgacatgt ccatcaaaga agccacctta attagcccga
     3121 tcgacggtgt cgtcgttgct cttgaccaac cttttgctgg ttccaatgtt actccagcca
     3181 ccgccacttt caccatcgtc aatcctaaca atctttattt caaatccgaa atcgaccaag
     3241 atattgttac caaggtcact atcggtcaag cggccaccct agagcttgac agtttcccta
     3301 gtgagatagt caattccaaa ataaattata ttgcttttac cccagtttct ggacagactt
     3361 ccacagtcta cgagatacgc ttcgacctcc ccctcaaaaa cgacaaatta acttatcgtc
     3421 taggtatgga tggtgatgtc gatattattc tttctcaagc cgagaacacc ttaactattc
     3481 ctaccgatgc tgtcaacgat gatgatggtc aacgttttgt ttacatcaag tccgacaaag
     3541 aacttattcg tcgtgacatc aaaacaggga ttgaaaatga cattactact caagttatcg
     3601 aaggactcgc acccgatgat caagtcgccg tcacccaaaa atagccccgt catcctcgaa
     3661 ctgcgtgatg tggtcaagac ctatacctct ggcaacactc agtttaacgc tcttgacggc
     3721 gtctctcttc agatcagaaa aggggagttt gtttctatta ctggtccttc gggctctggc
     3781 aagtccactc ttatgcatat tattggcttg ttagacaatc ccacctccgg gcaagtattg
     3841 ctcgaagaac aggacatttc tcatcttaat gaaaacgaaa tggccaaggt acgaaacgtc
     3901 actcttggat ttgttttcca acaattcaat ctccttccca agacctcttc tttggaaaat
     3961 gctatgctcc cgctacttta ttccgacgtc cccaagcccg cccgtgaagg tctggctatc
     4021 gagatgttga ccaaggtcgg tctcgatcag aaactcaaaa acaccccagc tcagctctca
     4081 ggtggtcaac aacagcgtgt agctatcgcc agagccttaa ttaacgaccc aaaaataatt
     4141 cttgccgacg aacccaccgg caatctagat tcaaagtctg gcacagaagt catgaaactt
     4201 ttccaccacc ttcacgaaaa agaggggagg accattgtct tagttaccca cgacccagaa
     4261 ctagcccaac aagccaaccg agtcattatt attaaagatg gaaaagttag cacagaaaac
     4321 aaataactta tgttaaaacc atccttagac gatctaatca aatccagcat tcgttctatt
     4381 ttaaagaata aaggtcggac cattcttacc tctcttggga taattatcgg cgtaacttca
     4441 gtaattttgc taacctctat tggtactgga ctcaaaatct atatcaccga acagttcgat
     4501 agtctcggcg ccaacaccat cttcgttgct cctggcaaaa tctttgacga caatggtggc
     4561 tttagtagta gtggtggcgg agctggtcga tttatttcca ctagttttag tcttgaagat
     4621 atttccaatc tgaaaagatc acttcgaggc aataccattg ttcctgtcgc tgtcgcctcc
     4681 gccgatatca aatcacctaa tgccaacaaa tcagaagtta gtattattgg atctacctat
     4741 caatatggtg ccagctccaa taacttaccc tccgccggca atggccgttg gtttaccaaa
     4801 gaagaagaag acaaaaaaga tgctgtcgtc attctgggca acactatcgc caacgatctc
     4861 tttccttcag gcaatgcact tggtaaaaag attattatta agagcaagaa ttttcgagtc
     4921 atcggaattc tcgatgccaa aggtggaagc tttggtggtc cagatgttga tcaatacgct
     4981 ttcaccccta tcggcatagc ctttaattta gtaggtaatc aaaaagttaa tcaaatcctt
     5041 attaagactc ccaacaagga cttggttgcc gagaccaagc tcgaagtcga gaaagtcatg
     5101 ctcaaacgtt ttgaagaaga tgccttcacc gtctttgata gtagccagtt attaaatagc
     5161 atcaattcta ttattggcat tctaactatc ggtttatccg gaattgccgc catatcatta
     5221 gtcgtcggag gtattggaat tatgaatatt atgttagtta ctgtttccga acgaactaaa
     5281 gaaattggtc tacgcaaagc cattggagcc taccctcgag ctattcttat ccaattttta
     5341 atcgaagccg ttatactctc atgcattgga ggattcatcg gagttttact tggctggcta
     5401 ggatcactgg ctatcaataa cttctttcct gctcaagtca ccctatcttc tattgccatt
     5461 gctttcggtg tttctgccat agttggtatt atatttggtg tcgctcccgc tcgcaaagct
     5521 agtaaacttt ccccaattga agcactaaga tacgagtaaa caatctgcta atattaaata
     5581 gtgaatagtt cagaaatcaa aaagcactta ttaccggtat tgattgtttt tgtccttatc
     5641 tccgtcattt ggattatcaa tcaagtccca tttttaagct ttgtccaact cgccctcggt
     5701 cttttaattg gagccttttt ccttgatata gatcacatta tttattggct ctatatcaac
     5761 cccaacaccg aagaaagccg actagctcag atcgccctaa aaaaacgcga cttcgactcc
     5821 atcatcaaat taatttggtc cacccaaaga gatcacatta acctcatatt tcaccacttt
     5881 ttctttcagg tcgtgttagt ccttatctct gttttcgttt ttaccagtac ctccaacact
     5941 cttgctatgg cctttctcat ctcgctcaat attcatcttc ttgtcgacca atacgaagac
     6001 tatcaacaaa atcctacaca tctccagcag tggctttttg ctagagaaag caaacaactt
     6061 cccattacct acctcggcca ctatctcggt gtctttgtta ttctgacact cctcctactc
     6121 ttacttctta taaactcggc cctatgagtc agctctcacc agagatattt ctcgaggcca
     6181 aaaaatatct ttctcccgaa gcagacaggt tagtttccga tgttcaaata gtgttagaag
     6241 acctcgaagg ccacacagat attagcgact actcctttat tgtcgcccct ctctcaaaag
     6301 cttacgaagg ctatctaaaa agcttttttt taaagttacg tattatcgat caatatacct
     6361 acgacagcga ccgttttcgt gtcggtaaaa ctttaaatcc atctctccgc tacaaacgct
     6421 tttcagtttt tcaaagactg gctgaagttg gtgaaggtgg agaagaactg gccgagatac
     6481 tttggagcgc ctggaagcat ggtcgaaatg aaatatttca ctttttcccc aatgatctaa
     6541 aaaatatcga tcggaacaat gcggaggaga ggatatccct tcttctcaac gccatcatca
     6601 agtcagggga atttctcaag acaaatcaag agaatattga tctgttataa tttaaaacat
     6661 atggcaaaga aaaaagcagt cgtaaatcaa gatacttgta ttggttgtaa tacttgtccc
     6721 ttagtcgatc cagacacttt tgaaatggac aatgacactt tcaaagctaa agtcaaagac
     6781 accgctccag aagttaatga caagaccgaa atggctgtaa cctcctgtcc ggttggtgct
     6841 attagtattt ccgaagaata gttctctaat acgattatga aaatatccac ccccgctgtc
     6901 gttttcttct ctctacttct tgctggaatc tctttttatt ctggaatgaa ttttagtaaa
     6961 acaggatcaa ctactactcc cgatatcaaa gccaccacca ctctcattac ctcaaaatcc
     7021 gacaagccag aactaaaatt ttttgtcatg agtttttgcc cctatggtaa ccagatggaa
     7081 gatgtccttc gtccagttta tgatcttttg caggacaaag ttgcccttac ccctcagtac
     7141 atttttgaca agatcgaaaa cttaggtgaa tattgcaaga cccgatctgg tgaccccgct
     7201 caatgttccg cttacgtcaa gaatggttat ttcaagaccg aagctgaatg taaaaaagct
     7261 atcggggaca acctagcttc ttgccaagac gagaaaaact atctcaaatc aagtacagga
     7321 gtcttctatg gttcacttca tggacgcacc gaagccaatc aaaacattcg agaaatgtgt
     7381 gcttggaaac aagttgccga caagaaagtt tggtgggatt tcgttggcaa cgtcaacaaa
     7441 aactgcactt ctgacaatgc tgatacctgc tgggaagaac aaggtaagtc cgttggttta
     7501 gatactcaaa aagttgccga atgtttcaat caagaagcct tcactttaat cgaagaacaa
     7561 atcgccgaga ctaccaagta caccgtcagt ggatctccca ctgttatgat caacggaatt
     7621 ttattcccac cagaatcagc ctacactcaa gatggcaaag gtagcatttt aattggtaaa
     7681 aaagttgcta cccaagatag ataccgcact ccaaatgtca tcaaagaagc cgtttgtgct
     7741 tcgatgaata aagctgccaa agaatgtaac accactctcc cagatctttc tggtgctgct
     7801 cctgctgctg gaggttgcta aaatacccca tggcctccaa agaaattaag ctcaattctc
     7861 atcagcttcg ctctagcaat cagctaatcg ctaaagttca acaacgtaaa gtcaaacgca
     7921 atccaattgt tttagttctc gacaatgttt tggacactta caatattggt tcattctttc
     7981 gtctagccga cgctattggc gccgaaaaaa tttatctttg tggaccagta gtcaccccac
     8041 caaatatcaa gattcaccga gccagtattg gtacttggaa atgggttcct tgggagcatt
     8101 ttccgaagac tgacgattgt ctgaagcaat taaaaaagga tggttatcat atcgttgcct
     8161 gtgagcagaa accgggtagt attatttatc ataaagcgaa gtataaattc cctctggcca
     8221 ttgttgctgg tagtgaatcg gatggagtag gggagtcgac tctaaaactt tgcgaccaaa
     8281 ttatcgaaat tcccatgtac ggcatcaatg tctctctcaa cgtattggtg gcaacttcgg
     8341 tcatatgtta taatatatta tgtcatcact atacctccga gacagagaga taaaaagcct
     8401 cactgaacca agtcctacaa agaaagacat caaaatttta cacgcctctt caaatcctgc
     8461 aagaatacaa gccgctgcaa attcactaca aacagaactt gataaaccaa gccaatctgt
     8521 tacgcttgca caagcacaag catttataga tcatgatcaa cgaatcgaga cagaatccct
     8581 aactattggt cgtgcgtcgt ttccagaagg cgaagcagaa aggggtatct ccatccttcc
     8641 ccgaagaggt gccatggata gggtagtcga caacatcaaa gctctcaaaa ttaggctaaa
     8701 ataacacttc tcttacacat taatagcgac gagtattatc ccttaccaat ctttccggtc
     8761 caagtctaac tggtcgtcta accaccacag caccatcctc aatatctgcg acatattgac
     8821 caaccacagg acaattttta aacaaattat cactcttgtc atgttccaca acaaaaacca
     8881 cgggtggtag ctctccctcc tcgctcttaa atctctgttt taattcaaat gccagtcgtc
     8941 ttggaagtaa accgcggtcg gtagccagtt ttttctcacc attcacaaaa atttctaacg
     9001 actgcctcgg ttctggtaat ctatccatat ataccccgat catatcacac cctacatact
     9061 aaataaatat cgatcagttt ttaggataac catagaaaat atctcaatta ttatccctta
     9121 aaaccggcca aaaatagcca tctttttgac ctcttttgac acactttgtc accataagtt
     9181 atgcctatct ttacgtacat tttgcttatt gccaagccaa aattaagtgt tacaatgaaa
     9241 ttatctcaat aaattcgcaa aattatctag cgaaccagag ttctagcgca ccagcgcact
     9301 acacatggct gatcaaaaat acacatctga acaaattgtc gtcctcgaag gtctagagcc
     9361 ggtccgtaaa cgtcctgcca tgtatatcgg atctaccgat gttaggggat tacaccactg
     9421 tcttaccgag ataatcgaca attccattga tgaagctctc gccggttttg cccacaatgt
     9481 ttggataatg attcatcccg ataattctgt caccgtcgct gatgatggtc gcggtatacc
     9541 ggtggacatc atgcctaagt acaaaaaacc tgcccttgaa atcattatga ccactctcca
     9601 ttctggaggt aagtttgaag gtgctgccta caaagtctcc ggtggtcttc atggagtcgg
     9661 tgccgcctgc gtcaacgctc tctcatctct ctaccaagtc gaagttcgcc gtgatgacaa
     9721 gattcacttc atggaattca acaggggaat cgtcaagaca caattgacca ccattcctga
     9781 gaccaaaata cctcgtcttg gacagatatt accccccaac accaatggca ccaccgtcac
     9841 tttctatccc gatacagaaa ttttcaaaga aactgtcgag atggattcca agaccatcat
     9901 tcgttcagtt aaagacagag cctatcttac aagcaaaata tactttcact tttacgacga
     9961 aagaaaaaaa acagaacacc actactattt tgaaggtgga attctctccc ttcttcgcga
    10021 aatcaataaa aacaaagtcg tcgttcacga gccaatttat cttcacggtg agaacgatgg
    10081 agtcgacgtc gaagtagcca tacaatataa cgatactttc caagaaaaca ccccatcatt
    10141 tgtcaacatt atcaacactc atgaaggtgg tactcactta actggtttca agatcgccct
    10201 caccaaggtc atcaaagact acgccgaacg aaaagaaatg atcaaaggca aagacgatgc
    10261 cattaccagc gaagatgttc gtgaaggctt gacagctatt atctctctca aaatgggtag
    10321 taaagacctc cagtttgaag gtcaaacaaa aggtaaatta ggcaactccg aagtccaacc
    10381 actcgtcacc aaaatcgtca gagaacagct cgaaatgtat ttcgaggaac atcccgatat
    10441 cgccaaagcc attgtcagta aatctatttt agctatccaa atccgtaaag ctgcccgagc
    10501 cgccaaagac gctatcatgc gtaaaggtgc ttttgattct atgggtcttc ccggtaaact
    10561 tgctgattgt caatccaaaa aacccgaaga atgcgagatc tacatcgtcg agggagattc
    10621 cgccggtggt tccgctaagc aggggagaga tcgcaaattt caagccatcc tccctctttg
    10681 gggtaaagcc ctcaacactg aaggtatgcg tcttgataaa atcgtcggct ctgacaaatt
    10741 aaaggatctt attattggtc ttggttgcgg catcggagaa actttcgatc tcgccaaatt
    10801 acgttaccac cgcattatta tcatgtctga tgctgacgtt gatggcgctc acatcgccac
    10861 tcttttatta acatttattt tccgccattt accaggaatt gttgaagccg gccatgtcta
    10921 tattgccatg cctccacttt tcaaaatcaa acaaggcaaa caaattggtt atgtctacac
    10981 cgaagaagaa aaagatgttt acctaaaagg tagcatcgac acttccaagc ctttcgatct
    11041 tcagcgctac aagggtttgg gagaaatgaa tcccgaacaa ctttgggaca ccaccatgaa
    11101 tccagagact cgtttactta aaaaagtcac catcggtgac gcagtcaaag ccgacgagat
    11161 cttccgtatt ttaatgagcg aagatgttcc acctcgaaaa aagttcatca ccactcatgc
    11221 tcatttagct acattagata tatagaatat gcccaaagac accaccaaaa acatattagt
    11281 caaagacgat gctcttctca tcaacaacga aggcaagatc atcaatgtcg atatcgtccc
    11341 tgagatggag aagtcctatt tggactacgc catgtcagtt atcgtctcca gagctttacc
    11401 agatgttaag gatggtctca aaccagt
//
LOCUS       DDWP01000016            5517 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_46464, whole genome shotgun sequence.
ACCESSION   DDWP01000016 DDWP01000000
VERSION     DDWP01000016.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_46464"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1601 a          856 c         1326 g         1734 t
ORIGIN      
        1 ttatgttatc atttataggt gaaagtattt ggtaaaaaaa ttaataataa gtcgttaaca
       61 aaagttgtct tggtaataac catgatggct ttgatgttta gtagcataat ccccttctta
      121 gatgtaataa gccaatgagg aatcggttgt cgcttgtata ccaaaaagtt attttctttt
      181 ataacttagt tatgaataaa actaggcaag gattaggtcg ttttaagtca gcattgaaaa
      241 ctagacggtt ttggtaccgg gcggtaattg gtcttctggt gttgacggtg ataagtgtat
      301 ttgcgttatt ggcggtgttt gcttggtttt caaaagattt gccgacacct aataaagtag
      361 ttaggagaga tggtttctcg agtcgaatct acgataggaa cggtgaattg ctttatgatg
      421 tttacaagga tgctaagaga gtgcctgtgg tatgggaaga tgtcccagag tcattgaaga
      481 atgcgacgat tgcagtggaa gataaggatt tttataaaca ttctggtttt gatcctttgg
      541 ccccatttag aatagttaaa aatgtgtttt attttaaaaa actgactggt ggttcgactc
      601 taacccaaca attggttaaa aatgtattgt taacttcgga cgtgacggta actcgaaaga
      661 taaaagagtt tattttggcg gtgcaaatag aggcgaagta taaaaagagc gagatattgt
      721 taatgtattt aaatgaagca ccgtatggtg gagcggcttg gggagtaggt atagcagcag
      781 aacaatattt tggaaaagct attaaggact taactttggc agaatcggcg atcttggctg
      841 ggttacctca gaggccgagc gcttattcgc ctttttcaaa aactcctcga ttgtttgttg
      901 atagaacagc acatgtgctg gaaagaatgg tagaagacgg atatattgat gatgctttgc
      961 gccagacgac attggaagaa gttaaaaatt accagtttag agatagtaaa agccaactag
     1021 ttagcccaca ttttgtattt tgggttaaag atgaattggc taaaaagtat ggagaggaag
     1081 tggttgaagg tggtggtttg aaaataacta caacattaga cttggctttg caaaataaag
     1141 ttcaagaaat cgtgactcag gaaattgata aggtggaaaa tctgggtatt agtaatggag
     1201 ctgccatagt tatagatcca actaatggtc agatattggc aatggttggt tcaagggggt
     1261 atggtagtga taaaactgat ggacagttta atgtggtaac tcaagcgtta agacagcctg
     1321 gatcagcaat taagccaatt acttatctga cggcgattaa aagaggatat actccggcga
     1381 caatgattat ggatacgccg gtaacgtttc ctggatctcc tggtcagaaa gattattcac
     1441 ctcaaaacta tactggtaaa tttgtaggac cgatatcttt agcggcagct ttgggtaact
     1501 caataaacac aacggcagtg aagttgttgg ctaatgtggg tgtggagaac atgttgcaac
     1561 aagcatttga tatggggctg accacttttg aaccaacgcc ggagaatctg cgaaggtttg
     1621 ggctagcagt aacactggga ggagctgagg tgtatatgat tgacttggct tcggcttacg
     1681 gagcttttgc taacggtggg ttgaaaactg agccaattgg agtacttaaa gttgaggata
     1741 gaaatggtag ggttttggag gaatttcgtc aaattgaagg caagagagtg atgactgctc
     1801 aagaagcatt tattatctct aatattttac aaaataatgc taatcgagaa ataacttttg
     1861 gagcagtgaa tggattgata gtgccaaact atcaagtagc agtgaagact ggtactacta
     1921 atgataaaaa agataactgg acgataggtt ggacaccaaa tttattggcg acagtgtggg
     1981 tgggtaataa tgataataca ccgatgggaa aagtggcttc aggtgtgtcg ggagcaagcc
     2041 caatatggaa aaagataatg caatatgctt tgcctttacg taataaacaa gatttttcga
     2101 tacctgaaaa aattgtcaat ttggaagtag atcgagtgtc tggatatttg gctcacgatg
     2161 gttttccggc ttacaatact tactttattg acgggacaca accaaaggta aatgatccaa
     2221 ttcatttgaa acttaaggta tgtaaagata agtcgggtat cgctccacca gaagatgtgg
     2281 ctaataacaa ttatgatgaa aaggaattct ttagttttag agaagaagat cccatctcta
     2341 aagacggaaa aaataggtgg caagagggaa ttgataaatg gatatcggaa caggttgata
     2401 aagataaata ttatcccccg acgggctttt gtcgaaacga tggaactttg acggttggtt
     2461 ttgatagccc gagtgataga tctacggtgg ggaacgatgt agatattaaa atatcgacga
     2521 gttcgttgaa aaaaattact gaagttaaag tgtgggcaaa tggttcggag ataaagtcat
     2581 ttagcgacag accttttgaa gggagagtaa acctgagtga tggtctatat acattgaaag
     2641 taaaagctac cgatcgtgat ggggctagcg tagagagaga agtcaagatt ggagtaaaca
     2701 aaccttggga ctgggcacca tccccgaccc cgacagaggt attgccaact cccactttga
     2761 taccggtatc accgaccata gggacgacga tacctttggt agtaacacct agttcgacat
     2821 agattgatct acaaagatat ccatagaata ggcagtattt attttgatat agttgttagc
     2881 agtcaccagt ggtgactgct aagtggtttt ttggcttttt ctttgtctgc cggtgttggc
     2941 tggcgagtga taatgtcggg aagaacgttc acctctgctg gcggcagatc ttttagctgt
     3001 tcatttattt tctgggcggt tgggccaact tttggccaga tgtctttttt catttgtgtc
     3061 taacttttaa ctctggtggg ttgtccgatt tgaagacggg ggtttgtggc cgctgttcag
     3121 ctgcaggaac ttgaggactg tttctgattc tagctaggac atctgacgtg tatatagttt
     3181 ctggcctgaa acctggttgg agcgtatctt tgttattttg ttgtgacatt gttttgttaa
     3241 tttatatatt tacctattac taggatttgt tgttttattt atttttaaga ataatcatag
     3301 gcgatattaa taattgattt atcccgatga accttcggtt atcgggattg tgtctttagg
     3361 caaaatttgg cgctatgctt caaatttcac ctaaagaaac taaaaaagcc tccttgcgga
     3421 ggctgttgat tttttatgac tctaggtctt ttatatcatc aacaatcctc cgcagtgcgg
     3481 ataagacgtt tgacaataat aagagacata aaggacatgg ggttatgata tagaaatcta
     3541 ctacgagtgt aaagggggaa aatgttactt atctttggac tctatgtaag cacactggtt
     3601 gtaccaatcc gccaaacttt tctcctgctt cgagaatggt catgtatcct gggataattt
     3661 ttacactttc gtctccaaaa gttattttac ttacttcctg gccgttatgt tcggtgtggt
     3721 cgactctggc tactctaccg aagtttcggt tttgggtagc tttttgttgt gttaactgat
     3781 gacatactcc tcgcaattca ggctctacat gtaatttcat tcccatactt ctagcaattt
     3841 caatttgacc catgatttct gtgggacctc caccaacaac tccagatggt ataagacgtc
     3901 tcgtttccat atcaaattat atcacaatat aggatactta ctagttggca tgctttaatt
     3961 tttagtttaa attaaaagtt tttagatgga tttttttggc agtcttgtcg tcggtgttgt
     4021 aatagcaaac tcggaaagtg tattttgatt caaaaatgtc ggttatttct atggaccaga
     4081 gaatgttctt gtctattaag gccgaatact ttttaatcaa gtctagtggc tctggcgagc
     4141 cgtcgatggt gacatttgcg tcgagggtaa ttatttgaga agttaactca acggcttgat
     4201 tgtcgataat ctggggaata tatgattcgg gtttttccaa atcatcaaga atagtttctt
     4261 tgaattcaag gccatcgtaa gtgccgaggg catacttctc aatgtattta tcgccatcct
     4321 tggaaaaaac tttgccgatt tcaaaaaatt tagggttagg gagtttgtaa cgattgtact
     4381 gatctaactg aagcttgagt gaaggaatta aactttgacg aaggtatatt acttcagcat
     4441 taatactatt ttcagttttt atgacagttt ttgggtcgac tgcttcggag acaagtggcc
     4501 aggtaataac ttcatcgtaa ccttcggaga ctagcttgtc ttttaattgt tcaattaaat
     4561 aaattatttt gggagtaatg tttttgactt ctttatacgt aagaggttcg tttttgggaa
     4621 tgttctgata accatagaat cggataactt cttcaactag gtcttctggg gcaacaatat
     4681 ctttgcgggt acctactcgg gcgacaatat cgagagcaac atcttcgggg atatcgatac
     4741 cagaaacaat agagggtagt tttggatcaa attcaattgg ggtagcaatt tcaggattgg
     4801 gatagtagtc aaaaacctct gagccaatag aaccaccaca gtgttcaaca attaactgag
     4861 ctaggtagcc gatggctgta ggaattgatt ctggatcgag aaatttttca agacgggtgg
     4921 ctgcttcggt ggtgactttt agctgatggc tgttgtgtcg aacggtggtg gggttgtaga
     4981 tagctaattc aatgatggta tcggtagttg aattgtctat tgaacatgac tcccctcccc
     5041 agaagcttag actaagggca cggtttttgt tgttgaccat taaaatatct ttgttgatgg
     5101 gtaaaacagt gccgtcgaga cttttgaatt cggtatatcc tggattcatt tgccagataa
     5161 gattgtcggt tgatttggcg gtgtcaaagg cgtggttggg gataccatac atgaacataa
     5221 cgtaattggt tagatcgaca agggtgttaa tactgttagt gccgtgaatg cggacaaatt
     5281 tttgtagcca ctcgggtgaa gtagagtttt taagatggac tattttgaca gccataacgc
     5341 gggtgacatt gtcagtctcg actttgattg gtagagagta atttgatttt ggccatcctt
     5401 cgtttgataa gctttggagt ggcaagttgt aaagaattga gaggtctttg gcaagaccgt
     5461 aataaccaag acagtcgccg cgattaattt tgatatctaa gtcaaaaaca atttcac
//
LOCUS       DDWP01000017            5558 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_27710, whole genome shotgun sequence.
ACCESSION   DDWP01000017 DDWP01000000
VERSION     DDWP01000017.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_27710"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1626 a         1442 c          894 g         1596 t
ORIGIN      
        1 acgggactta cggcgacgaa ctgagccagg aggacaaaaa acaccaccat ggcttgaaac
       61 aattttcgag ccattgcggt ttctcctttt ggtttttatt tcttattttt catttttgaa
      121 cgaccagctc aaggcaggtc aactaccctc agatggccgt tcaaaaacaa acctctttta
      181 tttacttata tactcaaata gcgtgtgttg ttaacgtgct tatttaacgc ttctacatgc
      241 ttacgcacaa ttagcattaa aaaaaccaaa aaagccctca aacaacaccg aattaacggc
      301 aatcatttag gaccctatga tgttctactt tgtagcgctg tgctacattt attaacatga
      361 ccgtttatac accacgaata cgcataagtc aataatataa gatagtcaaa aacatctctc
      421 cctaatcgtc tttcacgatc ggtcttgatt caatcgtcgc tgcagctgta acccccttat
      481 cttgatgcat tattcttctt tgttcaggtg tagtctgcac tggcttacga ggacgcgtca
      541 attccccgtt agaactcgtt ttagcctctg gaataactgt tggtactgaa aaaaaagaca
      601 tctggtccgc actgttatta tcaggtcggc cagatctagc catgatctaa gcaattacaa
      661 aaaccaataa ttcaactctc atttttagga taatacacca gctctttatt ctctgtcaac
      721 tattctgctt ttaccacttt ccaatcatct gttctctttt tacttccccc accaactaat
      781 gctggtaaca cttgccctct caccgcaaca acgtcatcaa ctttcataac tactgccgct
      841 aatggtttct cgagatccac cctcactcca gcatccgcat aacttttttt taactcttgc
      901 ccttctactg ccattttctg aattttttaa attcttaaat tctaaatttt taaattattt
      961 tttatatctt ctgcaaatgt tctgtttttt tccttggttt gtgagtccgc cagctggcgg
     1021 actcacattc ctcgggttcc tcagggggga agacaggttt ggtcaacttc attaacccgt
     1081 ctcccaccca tcaggccgca cgttttactt cttgttaatt ccttcgctct ggacaagagc
     1141 ggggtgcttt ccagacggtt tttgtgacgg gccttgaccc agtggaaggg tgctggcacg
     1201 aacacaactt gccggtacct tgctgggggg cacgccttcg gtgggacgaa tattcctggt
     1261 agacggagtg gtggccatta cttgcctcca aaatgaggct gatgccgaac cggacgacgt
     1321 tcatggtcac aatcaggtgt ggctttttga gctaccactg gcgcagactt gacgacaagt
     1381 ttcacaactg gtacaggctt tgttttaact ggctcagggg ccaacgcatg aaccatcgct
     1441 gcagcaccag ccggcgaaat cccatggacc gtagcatggt ccagcaacgc ttggtacaac
     1501 tttttgtcag acatgagttt ccctttctaa ttgactacag actgagattt tacttatttt
     1561 ctttactttt ttcttttctt tccttttcct cttttctcca tttaatcacc acactggtga
     1621 aatggaacca aatcacaact ataaaaatta tgaactagtt ccatttcacc aacactaggg
     1681 ataggaattt caaaataagg gggttttaaa aacgaacatt tgcagattgt tagtgtacct
     1741 tctctacaac aatcgcaaaa ttttcacttc tctgtttccg taccgcgatg gttacgctgc
     1801 ggtctgctgt ttcgaattgt agcgattata ccagatcatc ctatatatat ctacataaaa
     1861 attacacata ctaaactagt attagttgac gaataattta ttgcatcacc atttccattt
     1921 atccatccgc ttatcatcag gctctgttat ttccactcca cccccaggtg atacatcagc
     1981 cataatccta tgaaccaaca atctttcagg cttattccca tcaactcgtc cctctctttt
     2041 tagaacaatt cgtctcaaat ccttatatct cgttgccggc aacactacca cttgactctt
     2101 atcaacttca gattctctaa ctaatccaga ccgtaccccc tcctccacca cagaaaccat
     2161 ctttccaacc ctattttttt ccttactccc cctactccca aacgatctaa ttttctgaaa
     2221 accaccttct ggatcatgag tatcaaatct tttcgatgtc attgttcgga tattatccta
     2281 ctatcccata acttgtcaaa gcccaaaatc caagtccctc cttgtcaagg gagggattta
     2341 gggtgggttt tgctctataa taacttatgt ctaccgacca aaagctaact tttctcgaag
     2401 tccgcctacc tcaagacaac gaaactactc ttgaatccat gtcctctctt ttctcaaatt
     2461 ttacccaatt taccaagaaa tcattctggg atcgttttac cggtaaaaag cacactattg
     2521 cttccttaga gattgtcctc ctcgaaggac aagttcattt ttacgtcgtc acccctcagt
     2581 caaattttga ttttttccgt tcccagatcc tggctcaata cccttttgcc attgtcaaag
     2641 aaaccgaaga ctacttattg ccttttgtag agacgttgca cgcaacgtct ctaccatcat
     2701 ttactcagct ctccctcgcc cgcccctatt cttaccctct caagattgcc aaagactttc
     2761 gcgacaccga ccctctcgcc tcagtcctcg cccccctatc tcgtgccccc aatccaactg
     2821 atttttttct ctatcaaata cttatcagca atgccccaag caattggcaa aactctatag
     2881 tctccattat ccaaaatggt atcgtcgtcg acaaagaaaa aggtttccgc caagcccatc
     2941 ccgacaaagc cgtttttgag accaagattc aattccccgg cctccttacc caggtaaacc
     3001 tctttagcaa caaccctggc ttaatcagct ccgtctcctc ttcctttgga atctacacca
     3061 gccctcgtgg caattttgtc aaaactgcct caccaggcat gtttaccaaa aagaaagttt
     3121 taaaatctat ttttgatcgt caacttagta tcaatcatca agttcttaac accgaagaac
     3181 tggctgtact ctggcatttt cccaatcaat taactcgtct tcccaatatc gcctggggta
     3241 aaaaactcta cgccgaccct ccagaaaacc taccagtagc cgaaaacagc accgaagaac
     3301 aaaaacaagc cattactttc tttgccaaag ccgagtttcg caataaagac gccatctttg
     3361 gtatcaaaga aggagaagac cgtcgccgcc acacttatat aatcggtaaa tccggtactg
     3421 gtaagaccac cctcatcgcc aacatggcta tcgacgacat cagaaaaggt cggggcgtgg
     3481 ccattatcga cccacacggt gatttgtgtc agacaattct cgactatatc cccagcaacc
     3541 gcattaacga ctgttgttat ttcaatcccg ccgaccccga ttacgtctac cctcttaacg
     3601 ttctcgaagc ccagaacgac tcccaaaaag aattagtcgc ctctggcgtt atttctattt
     3661 tcaaaaaact ttatggcaat gtctcttggg gtccacgact cgagcacatc cttagaaact
     3721 ctgttctgac cttagtcaac actcccggtt ccgatatgac tcatatagtc gagattctca
     3781 cgcataaaga atatcggcaa aaagtcgtta ccggacttca aaaccagact ctcagaaatt
     3841 tctggatcaa cgaatttgac cgaatggacg ataaattcca gtctgaagcc gtcgccccaa
     3901 ttctcaacaa agttggccag tttatttcta gtaccaatat taggaacacc atcgcccatc
     3961 ccaaatctaa aatcaatatc caagacatca tgaatgaaaa gaaaattttg attgccgatc
     4021 taagcaccgg caagctcgga gaagacaatt ctgccttact aggcgccatg ttgatcaccc
     4081 aaattcagct ttccgccatg agccgagtct tccaatcaca agacgaacgg tctgattttt
     4141 atctttatgt cgacgagttt caaaactttg ccaccgaagc ttttattaag attctttctg
     4201 aagcccgcaa gttccgcctc aatctaattg tcgccaacca atacatgagc cagctcgatc
     4261 ggactatcca agatgctatc tttggtaacg ttggttcaat gctctccttt gtagtcggca
     4321 atcaagatgc ctaccttctt caaaaagaat ttggtgccaa attccccgcc gacgatttgg
     4381 ttaaaattgg taaatatcaa atgatttgta agctaagtat cgattccgag acaactcaac
     4441 ctttctatgc cacttccctg ccacctctta gttgtaaaaa ccaacaacgt gacaagctta
     4501 ttagaatatc ccaagaaaga tggggtaaaa agaaataaat atgacaaaaa aagaagaact
     4561 agctactgga aaacttaaag atggacgtca atttggcgca gccaaagtta ccattgaaac
     4621 tcccaaaaca aaaccaaatt acacttgtac agaatgtcat gctcccccaa cttgtcgcgg
     4681 aaaagcacca ggacaaaatt gtccagcagg atcacaaaga caatagttga atactcacat
     4741 caaacacctc taaaaagttg acatggtatg ttatactaac aaccgttacg tattagatac
     4801 atatgttaaa agacagaaaa ccacctgctc caattattga tgataaagaa ctacttagat
     4861 ggactcagag tgctcaacgg aatggtatgg atataccagg accttcaatt caagaaatac
     4921 aacaaggtgc tactaaattc gaacaaaaac aacaagccat taccagcgtt cttagtgcag
     4981 agttagccga tatgcgtaaa gaagctgccc ttgccgccag ggaacaagat cgcaactttt
     5041 tagaatcagc caaaaacaaa ccagaaagaa gaaaaaactt gtttaggcta ctaggcttct
     5101 aaagcttgtt tacatctttt ctccgcaaaa tctcctgacc ttatcaaaac atgtcattga
     5161 catagtgtta taagatatcc tatatttaat tattactaat taaatatttt atgagtgaaa
     5221 gattcacccc gcgattttta caatcagaaa gcaatccacc acaggaacaa ggtagattca
     5281 atgctgacat cctcccccgt caactcaggc attcattagc cgaagcaaag atgcaaatcc
     5341 agttacgata cattaatcaa agcattaatt ctaattaacc ttataactat gtcaaaaaca
     5401 acaacagaat ctcatgtatt caacaaaaac ggtcgacctc tagaaggtaa attcgccacc
     5461 tctacttatt ctcgccaaag tcttttcgat atgtcaaccg tgttccatta caacgatacc
     5521 ccttctctcc ccagatctcg tcaacaaact aaagaata
//
LOCUS       DDWP01000018            2651 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_36035, whole genome shotgun sequence.
ACCESSION   DDWP01000018 DDWP01000000
VERSION     DDWP01000018.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_36035"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          741 a          702 c          340 g          868 t
ORIGIN      
        1 cagccgaacc agccatataa ccaccagact tacctaaatt aacaaacact tctactggac
       61 catcaccttc ttcaaaaaat accgatgtat aaacctttcc cacatcacaa gcctttcgta
      121 acctcacccc tcttgccgcc tctggcgtct tctttttatc cggttttatc tcgactttca
      181 gactttcaga cttttgactt tccgacttta cagactgtat cacttctttt tcccttgacc
      241 cactccgata tattgtgata cctttggtgc ccaaggaata agccattaga taagcctgtc
      301 taacatcttc cactgtcgct tctttaggga agttaattgt cttactgacc gcattatcaa
      361 catattcctg aaaagctgct tgcactcgaa tatgccactc tggactaatc tccagagctg
      421 ttctataaac attctttata tcttctggca actcctctat cccctcaagt ttgcctccat
      481 ttttaactat cttgtctaat agcacctcgt tgtacaaatc tcttttacga agctcctcca
      541 caaacaccgg attcatcata taaagagttt ttccttctac tgtatttttc tgatatccca
      601 aagagaacac tggctcacaa cccgagctgg tatcagctat catcgaaata gttcctgttg
      661 gagcaatagt tgtcaaagcc atgtttcttg gccgataatc actattagca taaacactaa
      721 tatcccaagc tgggaagact cctctggttc tagctaactc ttgagtcgct tcttttgaag
      781 catcgtcgac aaacttcatt acttttcgtg cccaagaaac accctcttca gaatcataac
      841 gaacgcccaa tttaaaaagc atgtccgcaa atcccattac tcctagccca attcttcgag
      901 tcttactcac catatctcta attttttgca ccggaaattc atttacctcc accacattat
      961 caagaaaatg aatcgcttct tttgtcaccc tcttcaactc aggccagtct aaatcattcg
     1021 tacccacaaa tctcgccaaa ttaattgacc ccaaattaca cgcatcataa ggtaataacg
     1081 gctgttctcc acaaggattt gtcgcctcga ttctacccat agctggcacc gggttagatt
     1141 tactatcatt cattcggtca ataaacacca gtcccggatc accatactgc catgccaatc
     1201 gagtcaccag gttaaagact ttgtaagcat ttaatttctt agtcacttga ccagttcggg
     1261 gatttattag ttcatacccc tcaccctcac tcttcgcctc agcccaatca tacaattttg
     1321 ttacccattc ttcgattgtt cttagtctca aatctacgtc ccgaatcccc tcagctttac
     1381 gaatttcttc cactacttct tcaggaaatg aatcatcagt cacaaatttc ttgtccaccc
     1441 ccacctgttc catgaattta tttgtcaccg ccaaggatat gttgaagttt gttaaagaat
     1501 attcatctaa tttcaccact gaaaatctta aaacatcagg atgatcaacc gacaacatcc
     1561 ccatattcgc tcctcgacgg actccacctt gtttgatctg agaagtaaca tcgttataag
     1621 cctgcataaa tgacactggg ccaacagttg tcccaccaga agtagcaata tagtccccac
     1681 tcggtcttaa tcgagtaaat gaaaaaccag tacctcctcc actcttgtga atcttcgcca
     1741 tatcactcat cgtttccaaa atagaatcca ttgagtcatc aattggtagt acaaaacaag
     1801 cagacaactg ttgatgtttt ttaccagcat taactaaaca tggcgtgttt gggaaaaact
     1861 tttgactagc catcaattca taaaatactt tcgcctgtgc ctcaaaatct cctccgtcac
     1921 ctttagcaat gttgtaagcc actctccaaa aaatatcttt taccgtctct ttagtcttac
     1981 catcctcccc tttcatggca taccgtgtct ccgcgatata ttttgcgttg tccgagatca
     2041 ccggctccat taaaccagct ccattgtttc tattagttat atcgtccatt tatctttctt
     2101 ttttaaaatt tttaataatt ttttcaaaat cttctacttt tctaacatct aaataaattg
     2161 tggcaaatct gatgtaagcc actgggtcga tatctttaag cttgtccatg accatttttc
     2221 caatctctat tgaaggaatc tctacactct cccaattcaa aagtttcatc tccatctcat
     2281 caataatttt tgatctttct tcagagttaa gtttccagca agctttctca aaacaccttt
     2341 ccaatttctc ccgagaataa tcttccattc ttccatcctt cttaattatt ttcatctcta
     2401 cattacccat cttttcataa gtcgtaaatc tttttttaca tttcaaacac tctcttcgcc
     2461 ttcgggtagc ttttccatca aaagcatccc tagattcgag aacatttgat tcatcgtggt
     2521 tacaatatgg acacaacata aaaaattttt acaaaaattt ctatttttta attcaataat
     2581 tctcactcta tatatagtgg ataacgtcaa ctataagcgc tacccttggt gccgtcaact
     2641 agaaattaat a
//
LOCUS       DDWP01000019            4590 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_20608, whole genome shotgun sequence.
ACCESSION   DDWP01000019 DDWP01000000
VERSION     DDWP01000019.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_20608"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    3008..3091
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1248 a         1068 c          606 g         1584 t
ORIGIN      
        1 tatgccaacg atgcactttt cttgtccaac attcaccctt caatccctgc aaaagaaatt
       61 tccaaagttc aatcaaagaa catacataaa aacttaattt taattatgca acaatccgtt
      121 cttgccggtg gttccaccat gaaagacaac aagtatgttc gtcccgacgg atcttatggc
      181 aataatcaat tttcttttcg cgtctatcaa cgtttcaaag aaccatgtct aaattgtcaa
      241 actcccatta gtcgcattac tcttggtggt cgcggcacct tcttctgtcc caaatgtcaa
      301 ccggaatgaa gctcttctcc tcgtcttatt gcctctaaca catctccatt gaagttttga
      361 tctctgtata agttttcgct acacaaagtc acgtggatct tgacccccgg attactggat
      421 ttctctatgt ctcttgccag ttcttgaaca catttattaa tacgatgttc ttcagatctt
      481 ttgtccaagc gctcaatatc tatatcaacg actactgcca catcagtgtc agagtctggt
      541 ctgtccgttc tgtgttgtac tgttccaaaa aggattgatc tcttatttac atcacccaaa
      601 acttccccat ttaacctctt ggcaaacaat ctcgctgcgt ctctcctttt ttcattaaga
      661 ctcatgggat ctaaatactt gaagtaattt ggcttacaaa tttctttatt ttttcaaaaa
      721 gtctttcttc gatattgtca aaatcacttc cagcctcgtc attagcctta tctgatacca
      781 ccttcaaact cgatagtttt gacagttgtc catttacttt catgacttga gctatggctg
      841 tcaattccat ttccactaca tcgggggagt attcttcaat tatctcttta agctttggcc
      901 tttcatttac aaaaatatcc ccagttatac atttcgcctt ttttaatcca aaatgatctt
      961 ttaatttata tatatattct tgatcagggg atacctgacc tattttacat tctggattta
     1021 accctcttac atccaagtcg tggaatctgc actccccaac ttctaccaca tctccgaatc
     1081 caagttcgtt atttaaagat ccacacaaac ccacattaac ccatctttct gtctcaaaat
     1141 tacttattgc ccattgagcc cctgctgctg ccatggtttt tgatactcca atttccacca
     1201 gtccaagatc atccttttca tatatcggaa acactgtctt ctttgctgat tttataaagc
     1261 cattactttg aaacattttc tccacctcaa aacccatcgc taccagaaag acaattttct
     1321 ttttatcaac catctttgta ataatatcac cagccattcg tatcccttat tagcaacatt
     1381 ataaatttat attttatgaa aatattatct ctcatcctcg ttctcccttt attgctaacc
     1441 accgtcagtc cggttttagc cgagcaaaac aaacgtcaag aactcagaca agaaaaccaa
     1501 gaaaagcgtg tcgaaaccag acaagacaat cgtcaacaaa aatctcaagc cgctctcaaa
     1561 agacttcgac aggggatagt caatcgctac gaggctactt taaaaaacaa agcccgaatc
     1621 gaggctcgta tcgccgagat tgaagccaag aataatacct ctgaaaaaaa acgtgatctt
     1681 accgctgcta aggcaaaatt agccactttc aataccagta aataccttgc tgatttagct
     1741 gcttttgatg ccaagtctgt tgaggttgcc tcctcagaga ctccttacaa attaacttcg
     1801 gaaatgaaga ctcttgccaa aacccttcag tccgacatca aggatctccg tcaagttctt
     1861 atcgacagtc tccgtctagt aattaaggct cgttaaactt ttgtcctaat tgtagagacg
     1921 ccccggtggg gcgtctctac gtgttatatt taattaatta tgaaaatacg ttttattctt
     1981 gtttcttgtt tcatgattct tgtttccgcc ttaactctct ctgcttgcac caccaaagat
     2041 gcagctcctc aaacattcac tgaccagcct actgatatcc cagaacagac aacttcaaaa
     2101 atcactccga caacagtcga gcaaaaggct gatacttccg accagcagct tcttgatgag
     2161 ctgatgtccg acgacgcctc ttttgatgct gatcttaaaa atctagaagt agaattaagc
     2221 cagtaataga aaatactatt attatcgtca aaagcagatt tttttgccag gattttttat
     2281 agttatcacc tatttttagc caaactacag atccagttat cagaaagaat acactagcta
     2341 ttagccaata ataagggaaa tcttcgtaaa atacctgcca accaatatct ccgtattcta
     2401 ttaattcttt tggattatat atctgtagaa aatatcctat cgacactgtt gccagcatca
     2461 tcaatccaat tgatgtcatc cacatcccta gctcagccca ctttgttatc gtaaattccc
     2521 acttcggttt catttttacc tctcctgact ttattttgtt tagcacatta ttctttttca
     2581 taatatcttt ttaatttttc tttcgctcta ttcaaaataa caccaacatt gtttgtagtt
     2641 atgcgtaaaa tatccgatat ttcttcatag ctcttctctt caaaaaagta taaaactatc
     2701 acttctctat attttaactc caatttacca attgctttgt atagctcttt tttattttct
     2761 ctttcaattt ccaattcttc aaataacttt ttatcatctt taacatcttc aagctcatct
     2821 ccggctaatt tgggtttttt ctttttaata taatctactg ttttgttatg ggcaattctc
     2881 aatatccagc tactaaattt ttgattatca tcaaatccct gtaaattttg atacgccgcg
     2941 atcaggcttt cttccaccaa atcgtcaact tcagcattcc gaccaatgat tttatagata
     3001 aagcctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagttgttcg taccaagtaa aaggcttctt
     3121 acacaccggg caagtgactg ggggagtctt gccaatatga atatttccac aattaacaca
     3181 tttccattct agttctgcct cactatcaaa caaagttttt tcatccattt ttcgggcaat
     3241 aattatatat ctttcctcat gctctgcctc aacatgtttt aagtttccaa acagtcgggt
     3301 tgcttcttta tctccatctt cgatagcttg tttttcaaaa tctgggtaca ttgtcgtcca
     3361 ttcatatttt tcaccttccg ccgccatctt taagttatca attgtcttag caaaaggttt
     3421 gatatctaga tcggtattaa ctctcacttc tccactaatc aattcaaaca attcttcagc
     3481 atggactttt tcattttccg ccgtttcttc aaaaatacga gccacccatt cttgcccttc
     3541 ttttctggca atttttgcaa atgttgtata tttgtttctg gccattgatt cgccagcaaa
     3601 ggccttcagt agattctgtt gggtggtatt cattatttaa ttatacttca aaataacttt
     3661 ataaacttta taaactttat aaacttgact aacttgataa actaacatat gaattgtgat
     3721 tgcaactgca acgggaaatg caaaatgctt ggtggtcttg aacaacagat catggaggta
     3781 ttgtggcagt ccgataaacc attaaagcca gccgaggttc tcaaaaatat gaaaggggat
     3841 tacgcctaca ccaccattat gaccgtcctt aagcgcatgt ttgacaaaga attagtcaat
     3901 cgtgtctccc aaggtaatgc tttttattat tcttcttgtt tgtgcaagtc tgaatttgct
     3961 tctggcgcac ttgaagattt atttgctcga ttgtttagta cttatgggca agatgtcgtt
     4021 tccgctttcg acaaagccaa aaaaaagttt aagttttaaa cttctttagc tctgttttaa
     4081 catcttcaat catctgagta taaactttta cctgcatctc accatattca tccccatacc
     4141 tgcttcctcc ggctttgaat tctgcacaat cagtccgtaa ctgcagaatt ttcttctcat
     4201 atttttccaa ctcatctagt agtttttgtc ttttgtcttc cacattacta atttacaccc
     4261 agtttctgcc taaatctacc gagtacccac tcggtaattt tcccccaaaa caatctcttc
     4321 ttcaaactct ctattctcta cttttccttt ctttatttct ttcaagattt gtttttcaat
     4381 ttctccagtt gccacatttt catccaaatg ttcataaatg ttttcgtatt tttcccatct
     4441 gttttctcca aattcattct tttttatcaa ttcacctgtc gggtcttctg ccacacagac
     4501 gcagtagatt gtatcttcaa taactttgtt gtcgatagtt tttctaatac ttctccaatt
     4561 tccccaatga ctaaattcag ccaccaaccc
//
LOCUS       DDWP01000020           67499 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_7685, whole genome shotgun sequence.
ACCESSION   DDWP01000020 DDWP01000000
VERSION     DDWP01000020.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 67499)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 67499)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..67499
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_7685"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT        20418 a        12663 c        14589 g        19829 t
ORIGIN      
        1 ggagcgttga gataattgtt ctttggtgag tttttttgag ttgagaaata gttcgcgggc
       61 aggagtcccg gggcgaggag aatacttgaa aacatggatt ttggaaaaac caatatcttt
      121 acataatttg tatgtttgtt gaaaatcaga gtcttcttct tcggggaaac caacgatgat
      181 gtcggtaccg tatgatagcg ttttagaact ttggagtttt tgaaaaacat ctttaatttt
      241 tttaacatcg taagggcggt gcatgagttt gaggattttg tcagatcctg attgtagtgg
      301 aatatgtagg aactttgaga cacgcgggtt ttctattaac tttatgaatt tatcgtcgat
      361 gcataaaagt ggcactgaac caaaagaaat taactcgatg tttgtttggt ttaacaacgc
      421 ctcaactaga tttgataggc ctggtgtgta ttgggctaag ttaactccag tgataataac
      481 ttctttgtaa gagttggcag agtttatgac atcaatggca tctttgattg gcaggtagtt
      541 taaggtagga cggcggaatg gaacgatgca gaaagtacaa ttggtggtgc agccagattg
      601 gacttttaat atgtagcggt tggtatgaga aaatttatct tttatgtttg gggtgtaatt
      661 tattttaacc ttgtccttgt cgatattttt tagaattgtg acgttggaga taccttttag
      721 tttgtctaag ttggcacaac cggtggcgat tatttttaag ttagggttgt cggaaattag
      781 ttttttaatt tttgagattg attcgtattc cccttttttg gtgatggcgc aggtgttaac
      841 tatgattgta tctggcgttt tttcgtcaaa aacataaccc tggtcaacta gctgttggga
      901 tagttgattg gtttcagcgg cgttaactcg acaaccaaaa ttgagagcag agaaagtttt
      961 agccacgagg gtgtgctgac acaaaatctt taatgtagtc tagtatgtcg atagtagtat
     1021 cccatttaag ttcctgtctg gctttatctg gcatttctcc tgactcagat ctgccagaat
     1081 agccgtcgac gtatttgact tgccctccga aggcttgagc tatttcttcg acggagtatg
     1141 cttttttatt gcctagagca taaccatcac cgctgccatt ctcccctgct agaattatgc
     1201 ctctggccag gtcgctaacg tgggtgaagt ttcttttttg ggtacctggg ttcacgatag
     1261 tgagaggttc acctttaata taggcttgtt cgaatttggc aattagtgtg ccatatatct
     1321 gatcaccttt ttctcgtggt ccgaacgcat tgtagaagta gcagatggcg tagggtaagt
     1381 tgtaccaacg tccgtagtca ttaattaggt ctacgttggt ggctttggta aaagcgtatg
     1441 gtgtttggtg tcggccgtca ccctcaatag caaattttgt actggatgcg gcgtagacaa
     1501 gtttttttac tttacgttgt cgacaaaact caactacatt gaaggttccg ataatattca
     1561 tatcaaaaac tttgtttata tcatcaaaag atggagtaat tcgagcatat tcacctaggt
     1621 ggaagacaat gttgggtgtt tcagggatta acttgtcaat gtccttggtg tggccgtggc
     1681 ggtattctgc cccactaata tggttattgg tactaccatt aaaatagtta tcaagagaga
     1741 taactcgatt gtctgggtcg gagactagtt gttcgataat atgtgatcca acgtgcccgg
     1801 ctccgccagt taccagaatt acttttgaca taattgagta tacactggct gtaatttttc
     1861 cataagtatc gaataaaaac gtaaattgtg aatggtggct agtctgccgg cggtcatttc
     1921 tcctatttgg aaaagatgcc gaagataact gcggtcataa cgttgacata ataaacagtc
     1981 gcaagctgag ctaacaggag aatggtcgtg gtagtatttt tctttgtcgg gaacaaaata
     2041 ttggtaaaag tctttttgac ggaggtctat ttcgttaatg gacttggcgg tgtatgtata
     2101 cagtcttttg tgtcgggcat ctctggtggg aaggacgcaa tcaaatacgt tccatcccat
     2161 tttaaccatc tcgacaattt cgtggggctt accaacacct agtccgtaaa ggagataatc
     2221 tggtggggaa ttttctccaa taactctggg gatgtcgtaa ttgaattggc catcgtcttt
     2281 catgggccat ccaccataac caagaccatc aaatccaatt tcaactaatc tttcggtaca
     2341 ttcttttcgt agttgtaaat tgtgaccacc ctggacaacg ccaagaaggt aaggcttttt
     2401 tgttggatct aattttcttg attcacaaga ctttgtaaag atttttttac attctttggc
     2461 ccatgagatt gttctttcta cggtttctct ttgacgagat tcattggcat caagatctgt
     2521 aaagtcatcg aggacgacaa tcatgtctgt ctttaaaagc atttgaaaat cgatggacat
     2581 ttttggtgta aagagaattt ttttgcttgt tccgggaatc ttgaacttaa caccagcgtc
     2641 agagacagaa tctttgccgt taaattgttt ggctagtgac ataacttgaa atcctccaga
     2701 gtcggagatg actcctccac tccacgacat gaaatttcta acacctttgt gttcttttat
     2761 gactgagcct ccagggaagt tgttaagatg aaaagtatta actaatattc cggcagtttt
     2821 tgtactttct atgtcacttg agtcaagcgt cttaagtact gctctagtag cgtcagggaa
     2881 aaaaactggt aagggtatcg atttgccagt aatggtttta aagtcggtag gtttcataag
     2941 aaaatggtcg cctttgttat ttttcttaac tccggcgacc gacgttgtcg gggatactgg
     3001 attcgaacca gcaacctcac ggtcccaaac cgtgcgcgct agccaattgc gccaatcccc
     3061 gataaagtaa atgtatttta cactactggt gccgccagag gggatcgaac ccccacgctt
     3121 gttgagagca caagatttta agtcttgcgt gtataccagt ttcaccatgg cggcaacaaa
     3181 ggtattatag caattttttt gataaaatat gttcattgcg ggattagtta atcggtataa
     3241 cttcagtttt ccaaactgat atgaggggtt cgattcccct atcccgctcc agatgtgaat
     3301 aattttccga cagaaggggt ttatccccat ctatcgggct atttacaggt tcgagccgag
     3361 caatttcacc cttttgcaat ttatctacct gtttgaggaa gataaacgta tttagagcct
     3421 tgattcggac taatccgtct tttagctcga ggttcgagcc aagaatcctc agtatcatcc
     3481 ttctggcttc tgggtcctct ttagcatcat caaatagttg aattattttt gaggcaaagg
     3541 ctaaaacctc ttccatggtt ttggtccatg aatccaatcc atcctgggca atagcaccct
     3601 tagtttgcgt ctcctcttcg agtattctgc ttttctcctt ttggtattgg gcttcatcaa
     3661 tcatgccgtc aattttcatt ccatacaggt ttttcttttc ggtaaggatg gcctcaagtc
     3721 gtttcgttaa ttttcctttt tgattctttt caaattcaaa ttctttttcg tttcttcttt
     3781 tgaggttacc cctgatccaa gttattagat cgggagagat ttgcaggttg gcaacgaacg
     3841 ccagaacctg ttcttctagc tttatggcat taagatacgg ctgactacag tggccacctt
     3901 ttttggtaca gcgagcatag ataaagtttt gtgacttgcc agtagtttta tagtgcttgg
     3961 ttttgttttc agctgtgatg gatgcaccac actctccaca tttaagtact ctcatgaagt
     4021 agaaatcatt aaagtttttg aaaattcttt tccgaccgtc gatgatatcc tgaacacggt
     4081 gaaattcact gagagtaatc attggttttt gcttaccttc ataccgttcg ccattgtagt
     4141 cgaattggcc ggtatagaac acacgtcgaa agaaccgaaa ggcttcggtc ttgcttattg
     4201 gtttaccagt ccgcttactc cgaagtccca aggcagtgac ttttgctaga gagcttataa
     4261 ccgtctctgt cccagtgagc attaattccc accactttcg gcataggtcg aaacgatcag
     4321 gatcagggta aacgtccttt tgacccttag gcttagtttg gtcgttgaga tatccaagcg
     4381 gcgcttctcc aggcatccaa cccatcgtga gcttggtgtg catgcctctc ttaacattgt
     4441 cagacaaatc cttactaaac ttggtggcca taccaaactc aatgaaaagg aaggttgaat
     4501 tatttctatc ataatccatg gtaggcgtaa cgatttgcat tccctcataa tcaaccttat
     4561 caatgagcgt acctccttct ttggcatttc gacataatcg atcagccttc cagcaaagga
     4621 tgtgttttgc ttcatttcga agcattctgg acagtaaagc agtaaactct ggtcgcctat
     4681 cgggtctttt ggctgatttg ctttctctat agacaccaag aattgtaaag cccttatctt
     4741 cggctaattc ggtacatgtt ctttgttggt catctagaga aaggatttgg cggtctttat
     4801 cctcagagga ttttctacag taaataaaac aagtattcat agtttagtag ccatatcggg
     4861 taaatggctg ttggtgttga tgttaactcg ttgccgtgtt ttatcaagtt tattttcgat
     4921 cagatagtca aagaggttga gaagagcggt agcttgctcg aatgcttgtt cgtcggttaa
     4981 tttaactccg tagtctttga gagcaatttc tttgaagcga tccaactctt ctctggtaac
     5041 taacgaattt gttagtatta attggtttcc gtccgctact ggtggctgtg ctacagtttc
     5101 tgtcattgta tataacaaac gtacagaaac tggtggttta tctcaataaa ctttgtggat
     5161 ttttatgtcc acacaagtag actaatgtaa gtttctctcc aatttagcct gggaggtttg
     5221 attgacagaa aggggtcaaa tttggtatat tactagtacc gaatgatctc ggtggtgaaa
     5281 gtacctttta agggagcgaa tgtcggcaca gaaatgtgat ccgtcttggc cacagagatc
     5341 gaactttttt cagtaaggct tccacaaatc ttcttccctc tttttgatca gcttatagaa
     5401 cgagtcgtcg ttacggttat atttcctgta aattgcccca caaaccatat ccgccagctg
     5461 tagtagattg ttacttttgg agtctttgtg cctgatctgg cccaagagct tctccatatc
     5521 ggtattgagc ttttttctca agtacccgtt aaactcccgg ataaagtgtt tactggtcaa
     5581 atcgacgaac aatgatgcgt ggttaatccg attctgagct ctcataaaga gctggtcggc
     5641 caccaacatg tacaggctgt cggtgggatt gagtggctct ctttttagaa gcttggtctt
     5701 atcgataatc acggagcgat aatggaaatc aaagcgggac aactttcgca aaaacacttc
     5761 cttctcatgg cttgaagtac cggtgctgaa tttaaattct ctacctggat gaaacttcaa
     5821 ttccttcttc agatcgttaa tcgcctcgtt tgctttctcg gccgcttcta ggtcatcgaa
     5881 aataacgatg gtcaagacaa aataggaact agcgactctc ttaaagttaa atccggtgtc
     5941 acccgactca tccataaaga tgagcatggc tagtgacata gatccccctt ttgagttgtt
     6001 ccaaacccca atttcaaaaa atttttagta accaggaaat agtcagctgc cgttgaaaga
     6061 ggtagttgtt ttgttttatt gagtaattct cgtggcatta gtagttcggt ggcaaaacac
     6121 tgcgcttcat gttcctgttt gaaagtaacg aattgatcac aaccctcgaa atagaattga
     6181 tttccagtgt ggagaaaatg gtgacctaag gcgtgggcaa taagctcgcg tctttttcgt
     6241 ttatcggtgt gtgagcgaat agcaatacta tcgccgacat agacttcttt tactcggccg
     6301 cgaaatgacc actcgtccaa ggttatccct tcggcggtaa caattttatg taaatctatt
     6361 ggggcagtag aaagattgta cttgcgtaaa atctgtctag cccttttttg ggcctttatc
     6421 atattttttt cggcgtcgag tttttacata cccgatgtag tccatgaccg actccttctc
     6481 gctattggtc atgtcctgaa cttctttaag cattaacgta aatgaagaat cctgcttaag
     6541 cttattgctg gttttgtcca aatacccggc tttgtttaaa agcatctcgt tgggaatttg
     6601 atagacaaac gccagtgctt tcaactttaa tggcgaggga tgtaaatatt ttcctgattc
     6661 aagattagat aaataccctc gcgaaacagc tgactgggga tctagtttcc catttttaat
     6721 tgcctccttg gttgactcaa ccaccgaacg gagagtgaga tcttttgctt ctcgtaactt
     6781 gcacaaatac tcaccaacgg tagcaaaatc gtcaggtcct tttaatgtgg tttctttgtt
     6841 tgttcttatc ttcatatagg tcaataaact catcgatttt tattgtaaca gaaaaagtaa
     6901 gtaattactt attattcata ttataatgat agttgtctac ataaatggac acaaacaatc
     6961 ataaagttta ttgaagtaaa ttgcaagaat ttgtaccttt gtatataaca tgacaaaaac
     7021 gaaatctaaa aaaacaatat tcatcaataa ggacaagaga gttaataacg agttgttcga
     7081 tgaagtgaga ctcaccaaag gactatctcg atttcaattg gctgtcgagg ttgaactttc
     7141 tccccaaacc gttagacgag ttttagtttc tgatgaagac ccacgaccat caactgttaa
     7201 aaaagttggt gactttttag gaataccagc aaaagagtgg tacttaaaaa gagaaaacaa
     7261 ggaggtgaat catggataag gcaatgaaat ctaggatccg tgccaggaag cgaagatatt
     7321 atctctaccg cctaaagata tttatgtgga agtggttggc gatattactt ggcatctgga
     7381 ttatttacca gcttcttggg acatttttcc accgatgctg gttgtgaata taacaacaat
     7441 taatacagaa catgattatg tccagcaaaa agttaaatcg aaacaatgat ggcaagaatc
     7501 aactacacca caatcaatca cgaggctcga gtcaggttgg gtttaaccat taatgaatat
     7561 tgtgtgtacg atttaatcca caacttggcc agtaacccca aaaatattca gatgggctgg
     7621 tgttatgcca aaaaagaaac gatagctgat tacttagatc tgagtcgagc aacagtcttt
     7681 cgagccatta aaaagggctt ggaaataaat ctccttgaaa aacatcctga gcaacccgcg
     7741 ttcttaaaag ccactagtga ttggtacgac tcagtgatga ttaaagtcga gtctcaaaat
     7801 gagacggcta gtatcaatat gagacgcact ccgtctcaaa gtgagacaaa accgcgtctc
     7861 aaagtgagat caaacaaaga tatagagaac aatgaaaatg acaacacaga agttgtcaat
     7921 ttaaaaggat cttcaaaaga gatcgtttct attggccaag tgatcgctca tcaccagccc
     7981 agcttattag aagtaaataa cgagtgggaa gtggaagcca ttcgactgtg gcgtgagtta
     8041 aatttgtctg gcgatccgag cgaaccgttt gtaaaacaca tccatcgata catcaaaaag
     8101 aatttacagg taaagctctc tattgctctt tcgtattgca aagatgcaac taaagttcgt
     8161 gatcgagaaa agttatttta ttggcgctgt gctaacgagc cagcatgagc aaacattatg
     8221 aaatttatag atttatttgc aggaattgga ggatttagat atggacttga aaaaacaaaa
     8281 aattacaaat gcgtctggtc caatgaatgg gataaatttg ccaaccaaat ctatactaag
     8341 cactttggag agtgcgactc cagagacatc agaactgtcg atacaaaaga catcccagat
     8401 cacgatctcc tctgtgctgg ctttccttgt caatcattta gtattgcagg aaaaagattg
     8461 ggatttgaag acaccagagg gacgatgttt tttgaaatcg ctcggattat ccgagacaaa
     8521 agaccgagat attttctact tgaaaatgtt aaggggttac ttagtcatga cgaaggaaaa
     8581 actttccaga caatacttgg ggtactctcc gacctggggt atgagtatca atggcaagtt
     8641 cttaacagca agaattttgg agttccacaa aacagggaaa gaatattcat tgtcggacat
     8701 cttagagaaa caagtcgacc agaaatattt cctatcggcg aaagctactc agttcttaat
     8761 aagaagaagc attctgaaca agaaggaggg tcgagggttt cgagcacgat tgattcccgt
     8821 tatggatccc tcagaaactc tggagaaacc tacttgcaat acatcggagg tatcaaagga
     8881 aaacgagata tgtggataaa agacgataag caaaacagtc gaaactttag ccaaggtcaa
     8941 agggtttatt cgaccaatgg tattgcctca accttagctg gcaatgccgg tggcttaggt
     9001 ggaaagactg ggctatatgc aattccggtt ctaacacctg acaggcaaga aaaacgacag
     9061 aatgggcgtc ggtttaaaaa cgatggtgag ccagccttta ctttgacgag tcaagatcgt
     9121 catggtgttt atgatggcat taatattagg cgccttaccc ctcttgagtg tgagcgcttg
     9181 cagggatttc cagacaattg gactgaaggg attagcgata cacaaagata taaatgtctg
     9241 gggaacgcgg tgacaacaaa cgttattgct gaaataggaa aaagattaat ttttattaaa
     9301 ggaggtgaaa atatatgaca accaagaaat accatttcga gggaagcaaa aaagatacta
     9361 agggcaacac ggtgtatgtc gttagtgatt taaaaaccgg cgaacttctg gagtgggtcc
     9421 agaaaaccta cgatgcccat aaaggagaaa ttgaatatta aaacctatga ctaaaacacg
     9481 taatcaatta ctagaagaaa tttataactc tgttcatgag gaaaaacttc ggatggaagt
     9541 tgccattgaa acactggcag atgttgctga tgacaaaatc attgaaacgg tgactcatcg
     9601 gtcccctttg ggggctagag aagaaaacct caccaaaaaa gatgtgattg ccaaatatat
     9661 ccaggatatc gaaaagcgag aaaaagtatt ggtaattatt gaaagattat taaaacaaaa
     9721 tgaaaaataa agattaccca accatcgcca taagctctct gcgatttgca gagtataacc
     9781 cacgaaaagt tactcgaagt gtggtcgacc aattgaagca atcattgacc gagtttggtt
     9841 gtccggtgcc gattgtggtg aatagttata aaggtcgaga gaatgtggtt gtgggcggtg
     9901 agaagcgagt ccgggcagca accgagctcg gatggactga aattccttac agtctcgtta
     9961 atattccact acaaaaagag aaggcactca atctggcgct caataaaatc gaggatcaat
    10021 gggacgatga aaaactagct caggtaataa ctgaacttac taaatccgac ttcgatttat
    10081 cgctgactgg atttaatgaa gtagaaatta gcaatctcct ggataccacc atgcttctgc
    10141 cagagaacga atcagaggag gaatgggata ctcaaaaaga actcgacgag atctctaaac
    10201 cgatatccaa atatggcgaa gtgtaccaac tgggtccaca ccgactgatg tgtggggatg
    10261 ctaccaagcc agaagatatc caaaagttga tggacggcaa agtggcagac atagtgttct
    10321 ctgatccacc ctacaacgtc gctcacaaaa gcatggagaa agcgggtaaa tttcatacca
    10381 aggaagggat tatactaaac gacgatatga gtgatgaagc ttttatcaag ttcactcagg
    10441 gcttcttcac cagctttaac aatgtcctaa aaccgggtgg aactatttat gtttgtaccg
    10501 gctattcctc ctaccctttg ttttattacc agatgttaaa taacggtttt gttttcagtt
    10561 ccacgattgt ttgggtgaag ccttcctttg ccattggctg gggtgactat aaaaaacagt
    10621 acgaacaagt aatgaaaggt aaacgtggtc caggcaaatc aaaggcagag ccaatcttat
    10681 atggctggcg acaaggagag aatcataagt ttgaaggtga tgctaacgaa agtgatgtct
    10741 gggaaatgcc tagaaaagct gttactgaaa tggtccatcc aaccgagaag cctgaatggt
    10801 taattatgaa agcaataaaa ggtgggagcc gtgtcggaca gttggcggtg gatttattcg
    10861 gtggatccgg tagcactttg atggcagcgc acaaattagg gcgcatttgt taccttatgg
    10921 aacttgaccc aaaattctgt gatttgatca gaaaacgggc acaaaggctc aaaatttagg
    10981 ctttagctca agtaatgagc ccgcgtaatg ccctgtgtgg gcagattcaa atcaaggtgg
    11041 accagttaca cctgctaaaa catatgataa aacctaaacc aaaaaaacta ctaacactga
    11101 cagtttcaaa gatgcaagac gagacagaac agcaatatgc ggcatggctt ctttattgtg
    11161 aggcgggcag tattgataag acccaattgg tgtgggataa ggttggacaa aacctggatg
    11221 aaagttggat ggttttagcc aatcggctgg gcaaaaagcc tagtcataca acccttggtc
    11281 tgtggtcgaa gaaatatcgt tggccggagc gtcgtgatct gagattggaa gaagaaattg
    11341 ttgaattaaa ggataaagca gaccgcttcc gcaagaaaag aaagtacctg gtgacagaac
    11401 tacttatcca atcagtggcg aaggtgcaga aacaaatcaa acaaactccc atatcaatcc
    11461 tagagctgaa atacctttgg gagatgcata gaactgagta cgacgaatca attggaaaga
    11521 acgagatctc acaccggata gatgagagtg agcaggtacc acccactgat gaggaaaaag
    11581 agcttggtgt ggagattgat acagccataa aaaaatatta tgacaaacaa agataaaaac
    11641 agtgttctac actggatact aaacaataaa attaagaatg agaacggtga acccatcgaa
    11701 ttcgaggacc accgcttcat gttggacata tatgcagata agtcgtcagt acaggttata
    11761 aggaaagcgt ctcaggtagg agccagcaca atggagatcc tcaaggttat tcacgactca
    11821 cgcttctggg gcattaacca aatctacacg ctacccaccg gggatgatgt ccaacagttc
    11881 gtacaaagca aagtaaaccg gataataaac attaatccct gcatgctgga tggcattaac
    11941 cccaagagtg tggactccat agagcagaag cagataggca aggccttcac ttactttaag
    12001 ggtacgttta ccgagcgtga ggccatcatg ctgtcctctg accggaatat ccatgacgag
    12061 ctggataaat ctaagccaga ggtggtccgt gactacacct cgcgtatggg ttattccaaa
    12121 ataagaagcc agcacttctt ctctactcca accatccctg attttggagt ggataagata
    12181 tttgaacaat cagaccagaa atactggcga ttcaactgcc caagctgtca gtacaggcaa
    12241 cacatggagt gggagaagaa cgtcgacgag gctcgaggca tttatatctg tcagaactgt
    12301 caggcagaga taagccccaa acaaataaat gatggtggca gctgggaggc caagtaccca
    12361 ggtagagcaa cctctggcta ctggataagc cagttgcact gcccatggaa gacagcggcc
    12421 gacttaatca aagagaaggc agatgctgat gatgatgcct atttttataa ttttgtctta
    12481 ggcctaccat acttagctgc cgaccagaaa ataccagcca gcttattcct acgcaacgtt
    12541 gtggataaag aggtggataa ctttgatgaa tataacgtca tgggcataga tacagggaaa
    12601 ggaaccggta agggcaacca cgccatcgta ggcaaccaga agggcatctt ttgggttggg
    12661 atactgcaag atcatgaagg gcatgatcgc tggcaacaag cggccgagtt aattacattt
    12721 ttcaatgtca gagtcgtggt tattgatggc caaccttata ccgaggaagc tttcctccta
    12781 gccaagcaat tcccctacag ggtgtactta tcctggttta aagacgaccc caagatgcta
    12841 gaggtaatac gcttctttga tgagaaggaa aataaggacg ctgattttga agatgaggtc
    12901 aaagtttatt cctcccggac ccgaatcatg gatgacacca tttctgccct gcgccgagga
    12961 gagattcgat ttgccatgcc atccgacagc ccaaccttca aactgcttat cacccacgcc
    13021 cagaccatgt acgccaggac agtcaccgac aagctgggac aggcgaaacg agagtgggcc
    13081 aacaccggtc caaatgattt ttggttagca cttatatatt ggcacattgc cctacgcaaa
    13141 agacttaaat atgaaccaaa caaataacac accaaaagta ataacaactc cagtgacaga
    13201 ggcggaagcc aagatcctgg aggacatccg ccgggtgaag tacggacgag tggatgttta
    13261 tatccaggat ggcaaacctt accgtaagga aatcatggag caagaacgta taacaccaga
    13321 ggaaggtggt agcgcgggac aattcccacg gaaacaatct ggtatagaga tttaattact
    13381 ctccggacat agggataaat tcacataacc cccagcaatt cttatatact tagagcagga
    13441 gaaatgttac ttttccctaa ctcaaccgtt tactttaata gaagcggtgg agaatggatt
    13501 tattagtatg acgaaaccac cgtttgaaga ggtagttaaa aactatataa aactagttta
    13561 tttctttgcc aagaaatcct tgtctgatca ggcagacgta gatgacatgg tacaagaaac
    13621 ctttttgaag tctatgaaaa catacgataa atttaacttt aaaagcgagg gtgagttgaa
    13681 aagctggtta ttaaccatct gccgacacct aatcattgat cacttccggt cacagaagca
    13741 cacagtgacg atggatgacg aaaaaattga tctgtttggc aatggtgaga cagaagaatt
    13801 gctggaagcc caaattgata aagaagatag tgtcaagaaa atacagaagg ctctttcctc
    13861 gttaaagccc actgaaaaac aaatagtgaa cctgcgggta tgtgaggaga tggagtttaa
    13921 agagatcgcc accgccctat gttccaaaga agctgccgtt aaaatgcggt tttatcgtac
    13981 gttaattaag cttaaagaat cactcgtatg aaattcaaaa gtgctatcaa acaagcattc
    14041 gaacaagagg agctaaaaga agctaggcat attgatgacc tggagaatca gatattaatg
    14101 aaggtccgca tggagaaggc agaagaagct gaactcaaga aatattttag gccaatagaa
    14161 tcattttgga actttaagat tccttatgca ttcgcggtgg cagtgctggc ggttatattt
    14221 atcaccttca ctacctcatc tgctttggca aaaggttcgg ttctggaaat gctgataaac
    14281 ctaaggaatg ccttacaaca ggaattatca caactattga atactgatcc aacttataaa
    14341 gacaaaggga cccaaaaata ccagcaagcc caaaaggagt ggtgcttggt cagcgcacga
    14401 ccagcagagg cgcgtgagca ggcggtagaa gccgtcagag acttcctgga ccgacctgat
    14461 gctaacgtag aatacgaatg tgtaaggaat ccgagcaatg acactggcga gctaccacaa
    14521 accgaaagtt atattgtaga ttttgaccgc tttaccgttg atacaaaaac aaatcaaatc
    14581 attgaaatgg ctcccaccgg cggtcgatgg ggagaaaata aagacggaac taggtggttt
    14641 agcccgcaga aggaatatga ctacacgcct cgttatagcc aagcagaggc agaacaacta
    14701 gccagaagct ttgtcgccga tcatgaaaaa tcattaggta agattgacct aaataaactg
    14761 acgttggaga gcggaaagaa agacgatgga tccggcaaag taaactactt ctttatctgg
    14821 aaaactgatg gacaaggcga gaacataccc cagctaaata tctcctatac ccaaggtgga
    14881 cagttaataa gctatcttaa cgaattgggc aaataaccgt ctttacgggc aaagatgaag
    14941 aaaatgtcaa aaagggtgga tattacagtg attatgggta tacttggggt accagatcat
    15001 taatgtagct agcaaatata tgaaaagtcc aaaaatagta tttataacaa taacactact
    15061 gacacttcta gcttcatcat tttttgtaac aagtgcaccc cagaaagagt cgacaaccac
    15121 aatttttact gtttccaagg ctaaccaaaa acaaacaacc gagagacttg tcgaaagtgg
    15181 atatctaaga ttttcacttt ctctacctat tgccaggatc atgatgttcc ggtttggaac
    15241 aattgaccca gggggatata aaatttccaa gggaatgaat gctcgagagc taattgttaa
    15301 actgacatca gaacctcaat tgaagtggat tactataccc gaaggtcttc gtaaagaaga
    15361 aataggtgaa cgtctagcaa aagaacttca ttggaccagc gaagaattag ataaatggaa
    15421 tactacctat acagccatgc aatacgatta tagggaggga gtgtactttc ccgatactta
    15481 tctcatacct gtgaatgaaa atggacttga tactgctaag cggatgatta atcgttttaa
    15541 cgaaagattc ctaggatacc cagaaaaatt cacagcaaaa aacattaagt ggaccacggc
    15601 tctcaccctt gcctcaattg tccaacgtga ggctgcagga tcccacgaca tgccactaat
    15661 tgcaggaata ttgtggaata ggcttgacca aaataaacaa ctggaaattg atgctacagt
    15721 gcaatacgcg agaggtaaaa cagataaagg ctggtggtca ccaattaaag gcagtgacac
    15781 tagaacaata gagtcacctt ttaacacata tcttaacaaa ggtttgccgc cacacccaat
    15841 ttcaaatcct ggtatggatg ctattgatgc ggtattaaat ccagaagaaa cggactgtat
    15901 ctactatttg catgacaatg ataggataat tcactgtagc gcctcattcg cagagcatca
    15961 actgaatatt gaccaatacc ttcgctgatt tcctgactgt agaagctaat agtcaccact
    16021 aacaaacagc acttgacatt ctcaaactaa tttgtaatag ttgaagtatg agaagtaaga
    16081 atagtaagta tttacttata ctacacacgc ttcgttatct cccccataag acatccaatg
    16141 attgttctca aaactccctt ccttcgacaa cccaggttat tagtaataac tgggtttatt
    16201 tataaattag tgcttataga aaggaggtga aaaataaaaa agctatgaag tatccaaaaa
    16261 gaatgttagt aattcttccc ctacttgctg tctccgcatt tgtcggattc ggtctcgcaa
    16321 agactgatac cgtcggcgca caacaagacc caaaacagga gaggatagaa actctgcaaa
    16381 agaagtatct agacgctgtc gaaaatgcta agacaaatgg tgagttcacc acgaaccaat
    16441 tcggcgaaaa agtctacgct ggtaaaacag ctgacgacct aaagagttta gagaaagagg
    16501 ttggtaccga gatacgagaa ctgacagcgc gtcccgctga ggatagagct aaggcagttg
    16561 aagccgtcaa tggatggaat gataaattcc tctatacgat agacaaacag ccaaagcaaa
    16621 ctctccaata tgctggtcgg tcaggacaag ttcaaggcag caaactcatt ggcgcagagc
    16681 agtacttttc ggaagactat caatttacga tcgacccgca aaccaataaa gtcgttgaga
    16741 tgtatttacg tcccaaagaa gttggcgaac cggcagcgtt cgaagacatg accccacgct
    16801 ttactgaagc tcagttagag caaaaagccc gacaattgat cgaggcgcag aacttaggcg
    16861 tggatctaaa ctctttgaag ttcgagaaaa accataaggt aggaaccttt ttctacacat
    16921 gggaaggtga aaagatagca gacggagtac aaccctctct ttacgtggca ttcacgcaag
    16981 gtgggcaact gatcggctac atcaatgcag gcttttttga caaactatga aaaacatatt
    17041 ttcaaaaacc ttaatagcat tggcggtggt agcagtcatg ggctttacca gcgtgagtag
    17101 tgcattggcc tatggcgatg catctattta ctgcacgggt ggtggctact ttgacaagtt
    17161 cggtccaagt caatattgga ctgaatttac gagtatggga tggtgtaacg ccccagctgg
    17221 caacaactgg tgcgagaatg ggcaatgtgc ccaaaactca ccctacaata ctgtgactcg
    17281 cagtatgtgg agaacctact cagggagtca acagagtaac tacgcccgtt ggaatatggg
    17341 taacgatggt ggttggtatt actggtttgc ctttataccc agtaactatg cgactacctc
    17401 gagggcgccg tatacgatta cctacgcggg aggaagctcg tacaacttca ctgttaacca
    17461 gaacgcttat tcaaacatct gggtaactcc tggttcaaga actgagaatt tcaatcagat
    17521 catgaacaca tggttatctg atgcgacgag tgagagtccg tcacaacaaa tcggttttga
    17581 cgaagttcag aaatgttctg aatctggcca gtacagtgcg aattgtccag gcaaccatac
    17641 cagaaggtaa tggaggaagg tcaaggccag cccgtcaaaa ggctggcctg acccagcaga
    17701 gaaagaatat ttaaactaaa ataaaaaagc aatatgagaa acaaagagat aaacactaag
    17761 gagatcatcc tgatcactct tgcctgccta gcaatggcag tcggctcata taccttcttt
    17821 acccacaaag acactagatt cgaagcagtc gacctgacca accgagctga aggcaaggaa
    17881 gttgaagatt actttgtcga gaaactaggt atgaacagca cagaagctaa gaagattgct
    17941 gatgagggtg tgtcgtttta tatcaccggg aacaccacac ttgatgcaat tgtaagcaat
    18001 ttacactact acggcctggt aaaggacgag aaagctttca gagaggcatt gacgaagact
    18061 gccgacacgc tacctggtaa agagaatgcc atcaaggtag ggaataacac tatcgacatc
    18121 cacgcctact acggcctaaa aaaaggatta accgcttggc aagtcgcaga tgtgctactt
    18181 aatcacccat cttatctgag aggtgaggaa tataattata tttttatgcc gggagagtat
    18241 tattatgacg ctgcccagaa aacaccagca cgatgatggc aaaatttagt agaatacgct
    18301 tatgaagtta aaaacattat tagtatttat cgcagtaatt attggcttag gatttattat
    18361 caaaaacttc tctaatggca attcaaaaca ggtggcctcc cccacgccat tggaggcggt
    18421 taaaacgaca ccggatccga tcctgagccc aaccagagca ctcaagccaa acgaggagtt
    18481 tatctcgccc tttggtttat atgttattgt cccccaggat atgactttta gaaaagaggt
    18541 agcagacaat gatggcatta tccatagtgt cgggttttat atcgaaaata gcagtaaaga
    18601 taatccctat atgttgtatg gcctctacga agccaaaact gttgtcactg atcaaaccat
    18661 gacaagtgct aaaaaagaca tggtcccatc gacaatcaaa gacattacga ttggtgggta
    18721 taagggagtc gagggattaa taaccggcga caagaccagg tacatggctg tcattgtcaa
    18781 agatggtaaa aagatcacct tctcaactat tcccccaacc gaagcaaaca aagtaatcac
    18841 agatcagctt ctctccactt ttagctttca gtgaggtaga caaatcactc taattgtcta
    18901 atcgaccact tgctactatt ttctggttgc caggtaatcc atatttcctg aaaatatcta
    18961 tcaaaataac catccatttt gaatggggtg tatcttgtag aaaaggattc aacatcagag
    19021 tttgttagta aggggctatg aagattgcaa agtagaagga tttctcccgt aaaaccatca
    19081 tagcttttct tatttaactt ctctaatttt tttgctatct caagttctaa attttcaaat
    19141 gttactagtt taaactcagg tctttcatta tttacttttc ctcgtgcatc attccacatt
    19201 ttgcgagcct ggtattgatt gtcgtaagca ccaactaatt caatagcggt gttgtcactg
    19261 caaatacaat caggctcttt tttactagga tcacccaacc ttgtgaagca tagatcgctt
    19321 ttatttagat cgttatataa ggtcaagaag cgtattgaag ctgcttcttc atgagctgat
    19381 ttgagttgtg tatttatcat tactctataa gctaatttat ccatactcta aaacttctta
    19441 cttagccaat atatgattaa tccaacaatt gaagcggcaa caatctcctt aatgaatttg
    19501 ccgatccacc caatgaccac cctgattttc tgaaaaagga actgcttgtg aatatatagg
    19561 atgtccgcat attgcatcct ttgccagttc gtcaaataac ttcttctagc tctcaaaaat
    19621 aaaacatcat gctcgggtaa tttttcacct gcatttagtc ttaacaggat cttatcaaaa
    19681 atggtttggg cttctggatt cattgtcata attgtttcac ttatgttgtc ctaaagattt
    19741 gatcgcattg ttatacaggc aatagtcaca gtcctgtccc tgctctgggt attcgtctga
    19801 ttgtaggcac ttgtgagcct ctgtaatgac accttcgacc catgaatcat caccaacgta
    19861 gggaattaag gtaatatcaa attcaagctt gctgtcgaac gcttttctgt ctagctgtcc
    19921 attacaataa acaaaatagc cagtagaaga aaccttatag ccatttttac gtagtagcca
    19981 ttgatacacc tccatttgac gtttatagcc atcttgccac tctttattta actcagtaat
    20041 ctcttcactt tttgaagtcg acttatagtc aacaataata tattcgcctt gagagttttg
    20101 ccaaagatca tcaatggctc caaaaatcag gaggttggtg ggtttatgta aatactggat
    20161 accagtaaaa ttatgccgcc atttttctag atcagcatga gcaacaggtc ttgcatcaac
    20221 tccgtatttt tcaattaatg ggtgtttagt accattggca cggtgaatgt cgaactctag
    20281 cttgagtaaa tggtccactg cggagttgag agcaaaaggg aagcctggtg gacgtgccac
    20341 gcctaaacga cgatcaaaat aaaaacagcg tgggcaattt ataaaaagat caatttttga
    20401 tcgggacagt ttaaacggct cggatgagtt tggatcatat agccctttgg ttcgttgtga
    20461 gttatagtat tgagacataa ttaatcaatc aattatattt ttaggaacgg gtattgttta
    20521 ttctgattct taattatata taaattcttt ctaggtctcg ttatcataac gtataattta
    20581 ttcagttcct ggatataatc ataaatttca cctatgttag cctggtatgt tttcccccag
    20641 aaaattgcac tgtgtagtcc ataattcgat ttatgtggca agtcttcaat tttttggaca
    20701 atcacctgtt cgtattctaa ccctttaatt ttatgtatat atttgatctc aaaagtaagt
    20761 ttttctgtat ttgatctggt aatgtactgc agaaagccct cgagctttgg ttctgtgatg
    20821 ccagtgagcg aatccatttt gtctgattcg gaaattgaaa caaaaacaga ttgttgattt
    20881 tgatttaaac tcaagtttga ttttagactt gacactaacc gctcactgaa tgaaacaaat
    20941 tcgtcaaaaa taggcttgtc tacgtccaac gaaattgaca aatccgtgga gtctaccgtg
    21001 atgcgaagca aagcattatt ggatatttcg tagtttaatt tatcgaatgt taatgactgg
    21061 agcgaataaa catatttctc taaggctttg gggtatttta aacggttgtt ataaaagtca
    21121 tatttcaaga ctagatattt aatgaacaat gaaaagtact taaacccata tttgtcttca
    21181 tagtctttat aaatttctga gtataagcta aagttatttc cactatcagt tgtgtcgtgt
    21241 agtttatatt gtttgtatac atcgatagaa ttgaacctat ttattccgat acagcatacc
    21301 tcttcgttta gtagttcatt aaattttaga ttaacaattt cgctgactga agaaattgtg
    21361 gatttgataa tgaccttgcc atcaccacca cttccttgta aatcatcaga tatctctaga
    21421 tcatctagat atccgacgat ttcctgattg ttcaggtttt gaatgggtcc ggatcttttt
    21481 atttggttta aaacttcgtt atagtttttt cttgcgaaat taaaaacttc atttgaacat
    21541 ctatgagttt tttctaactt aatttcacag tcagcatttt tatgtaattt gttaaagaat
    21601 gccttcatat catacatact tctcggattc gctcctgcga agccatatat tgattgattt
    21661 ggatcgccaa ccatgaacca ctttacctca tttttctttt caccacggct tgcacttagt
    21721 tcctcaatta tcttgcactg tatctcagaa acgtcttgaa actcatcgat aaaaatccaa
    21781 tcgatagatc tattaagtgc ccttctgaca atacggttct cttggataat aaacagggag
    21841 agaaggttaa ttaggttaaa atcaattagt ttttgtgtgc ttaggaaatt tagatacgat
    21901 gcgatagtgg gtatttcatg agcattgcta actggtttcc aacttccatt aaccaatttc
    21961 aagttataat aaatttttcc actctcaaga atcttattaa tttctgactc ctcgatgttg
    22021 tgttttttca ggtggtcaat caataattgc ccatgctctt gaaagttaaa aatcgtttta
    22081 taaggtctca aacctttgct acttttatac aaataggaaa atggttgtaa aacgtattgc
    22141 aaacagaaac tgtgaaacgt aagaaactca actttagtta ataattcagg cttaagtatt
    22201 ttagagcaac tatcttcaag atcctccaca ttaacattgg tataagaaat acaacaaatt
    22261 ttattaatat ggggattatc taacaacaaa ttctttatct ttaaggtgag gattgtagtt
    22321 tttccactac caggtagagc tctcacaaga gtgtcttgat cgcgttcaat aatttcgatt
    22381 tgttttggtg ataaatcaaa atctatgcca agttttattt tgtaattaac catgataaac
    22441 catcttcaat atacttcgga ataacaaagt ccggtaaatc atcgaaggca gtaacactgc
    22501 tgcttttatc cacaacagaa tcaccattcc aggtttcgat ttccgccggg tcttgaagta
    22561 attttgcttt aagtaaagat gacaaatata acgatatgga tggtttagag acttcgttgc
    22621 caaggataat gtcaaaaaag ctgtcgaatt tgtccttgtc tatactatta gatttgactt
    22681 catctgcaaa cttattaatg gcgtcaattc ttttggagaa agtttcatct ttcttaaaat
    22741 aatcaccgtc cctcataaac ctgtatagga ttaacttcag atactcgacg ttatttgcat
    22801 ttgggttggt cggatctgga ataaaaaaac agtcaatttc aaaggtataa atatttcttt
    22861 ttgttactac taattctttt tcttgatcgg cgatttccgc atttaattct tcatattttg
    22921 ctttgtaatc aggtatcttt tgttcctcta agtgagagtc tccgtctcga agatttaaac
    22981 atttgttatt taagcctctg gtcaaatcat tattgaacaa agcattgaat ggatcaaata
    23041 tctttcctcc cacctccact atctctatac cttcactatc aatatcaata tttatatctg
    23101 gattaatgct ctttaaatat gagttgacta atgttcctag tgtatatttc tcagacaacc
    23161 cttccactaa gataattttt cgagcaaaca acagagaaga tctggtagcg tctagatatt
    23221 gtttaaggat tcttctgtta tgtccatatg tttcggaatt tgcttgtaac acactatcga
    23281 tgttaattgc ttttgcttga tggattcctt tttgtaacac cactaagttt ttgagatctg
    23341 atttagaagc gagatgtgtg gaatgagaag tttgtattac ttgagtaaaa gcaaaaaatt
    23401 gtttatcttc atcttcgctt tcttccccat tcgacttatt taattccatt aatttctgtg
    23461 ttttgttctc tatttgggtg tgtaggtatt tttgattttg gacatctaag tgtgcctctg
    23521 gctcttctat caaaagcaaa ttgtatacaa tcttaatttt ttcatctgaa tcattaagaa
    23581 ttaattcctt tttgagttca aataacgcga atattgccga gatgtacgct agattttggt
    23641 aacccaatcc gacaacattc atatcttgag atgtctttaa atcttcgttt aagttaacaa
    23701 gaaagttgaa gtatctgcca atattttcta gggtaacttt tggagttcca ataacaaggt
    23761 cagctctatt tgacaaaaga ttaatgtctt tatcttgaag acgcttttca atttcatcca
    23821 ataaaatgct tgcttctggg atagcgccac ttgttcgcaa attttttaag gcggcagaga
    23881 cttctttaga aacactgtta ttactattgt tcttagcgca ccctctaatt aattgtgaga
    23941 ctaatgagcg gtaaccaaag taaaagtcat ttttaacgtc tcgtaaagcg tcaagagcca
    24001 gtaaatttat gtgttttcta accttattgg cataggattg ttttgataat tgtttttcca
    24061 gaacgacttt gggattctct cggatttttt caatatcttc ttcactaagg taaaagtatt
    24121 tataaaagtc ttttttatct ttacatccgt gcaatgtcga aatgatcgtg tcgccgtagt
    24181 cttttgttga tccatcagga aggtaataat aacctgaagg gtgatatcta cctgcaacta
    24241 aaaatcctat gtagtcctct tcgatatcgt agcctccttc cttgttgatg gaagaaggag
    24301 cgaatacaaa tgatatattt gcctcaagat tatcctccga ggagtctaga tcaattccgt
    24361 tatttgtagg cgcaatagaa tataaaagtg attgaatttc ttctttgttc gagacgctgc
    24421 tgaaggttat agttataact atttcctcac cagcaaccca gcaattagct tccttactcc
    24481 aacaaaaatc gtcttccgat agtctcttgt cccaaggcgg catgcttgaa tctagaacta
    24541 gtcgaattgc ttgtaaaaaa ttagttttac cggagttgtt tcctccaatc agcgtaagtg
    24601 gacttccatc aggaataaac agatcaaact cttcaaagtt acggtaattt tttatgtaga
    24661 ctctagagat gtgcatagtt tattaattcc aatcattcac ctgatatatt aaaaaatctt
    24721 tcaaaaaatc ttgtgagttt gtcaagtacg ctttcacgct ttttggtacg ctcacctgta
    24781 ggagagaatc gagatactgg tggtaagatt tttgtaattg ccgtaccagt tgtggcgacg
    24841 ttaccgtttc ggaaagcgtt cttaataaac gtatatgttg cgtcatgatc tagaccttca
    24901 cttgtaatga tttgctcaag ttcttctacc ttcttgccct caataaattt tctccaatca
    24961 tcgtccacaa ttgaatggac atcaagcgat tcaataaatt gattgataag gtctttcttg
    25021 tttcgcaatt caacgctcga atcgatggct ttattaatat caagtagaat ttcacgattt
    25081 tttgtgtgat cctcgtggta cttttttatc agtcctagaa tgtaatcaat attgatatct
    25141 acctgtttaa taagctccat ctcaaatact acatcatcat taacattttc cttactgtct
    25201 tctgtgcctt tgcggaattc gttataaata tcaatataag cactgtggta atcttgaacg
    25261 tcgcgctcag ataagatctc attgcccaca aattcatcga aggtagtcag aatattgcgt
    25321 actcgcaaaa ttgccccata tagtttgata aagtcttttt ggttttgctc gccgataatt
    25381 ctttcaccga caggaaatct ctcttgcaat tcttttacta aactttcgta tcccctaatt
    25441 tctttgctgc cgtcgttgta accatggtaa taatcagcat atgccttcaa aagaacaatg
    25501 ccgctggctt ctttatctcc aaatagggca atggactcat ttgtggcttt ctcaaggttg
    25561 cgaaaacaga cgatgttccc aaacgttttt acgctattca aaatacgatt tgtccgagaa
    25621 tacgcttgta ataatccatg caatctcaaa tttttgtcta cccataaagt attgagagtt
    25681 gtcgcgtcaa aaccagttaa aaacatattg accacaatta agatgtcaat ttcccgattt
    25741 ttcactcgtt cgcttacatc cttataatag ttttgaaatt tatcagagga agtatcatag
    25801 gatgtaccaa acattttgtt gtaatcaaca atggcacttt ccaaaaaatc tcgggagctt
    25861 acatcaagac cactagtgtc ctccgagttt tcatctatca ttccgtctgc atcctcatca
    25921 tttacaccaa aactataaat agtcgctact tttagttgtt tgtcgcttgg gagatcagac
    25981 aattgttttt taaactcagc gtaatacttt ttggcgacat caatagaaga gactgcgaag
    26041 attgagttaa agccggctag tcttcgatcc tttaatttat aaaagctatt gcgtttagtt
    26101 ttctgatcga aatgttcacg gatgtatcga acaatattct ctaggcgttc agacgcagct
    26161 agaattgcct cacggtcaat atcactaact tttttgtctt ctatgttttc cgcttcacga
    26221 actgtcgaaa tatagtcaac tttaaacggt aaaacgtttt tatctgcaat tgcatctaca
    26281 atggtatatg tatgtagctt ttctccgaac gcttgttcgg tcgtcttcaa atctggacga
    26341 cctcctgatg aggcattgat tgcaaaaatc ggtgtaccag taaatccgaa gatatggtaa
    26401 ttcttaaatg ttttggtaat ggcggcatgc atgtcgccaa actgcgagcg gtgacattca
    26461 tcaaatatta ggactacatg gtcatcaaaa atggtgtgac cggtatttcg actgataaaa
    26521 cgatcgagtt tctggattgt ggtaataata atacgagcat ttgaattctc gagctgtcgt
    26581 gtcaaaatcg ctgtacttgt attactgtta gcagcacctt tttcgaattt gtcgtactct
    26641 cgcatcgtct gatagtccaa atccttgcga tcaacaataa aaataacctt atcaatataa
    26701 gacaatttgc tcgccaattg agctgtcttg aagctggtca gggtctttcc tgatccagta
    26761 gtatgccaaa tgtatccacc agcttctgct gtccctagtt tcttatagtt tgtactaact
    26821 tcaattctgt taagaattct ctctgtggcg actatctgat aggggcgcat cactagaagt
    26881 aatttgtcag tagtaaatac acagtaccga gtaaggatat tcaaaatcgt atgttttgca
    26941 aaaaaagtct ttgcaaaatc cattaagtct gtaataggtc gattggtggc atccgcccac
    27001 caactggtga attcgtaact attactggtt cgtttgccat ttttaacaga gccttcgttt
    27061 gaatccttta catgctggta tctggtagtg ttactgtaat acttggtatg tgtgccattt
    27121 gaaatgataa atatctggac atattcaaaa agaccagacg aagcccaaaa actttctcgg
    27181 ttatatcgat taatctggtt aaacgcttct tgaatcgcta ctccacgacg ttttagctcg
    27241 atgtggacta aaggcagccc attaacaaga actgtgacgt cataacgatt agcacgctgg
    27301 ccgtcttctg ttgcgtattg gttaataact tgcaggctat tgtcgtgaat tgagtctttt
    27361 ttaatgagat agatattttt tactgtgccg tcgtcacggg tgagattttt aatatgatct
    27421 tcttgaatcg ttgctgtttt ttcagtaata ctctgatttg gatttgcaag ttcacttaca
    27481 aaaaatcgtt cccattcgag gtctgaaaat gtaaatgcgt ttagcttttc taattgctta
    27541 cgtaagttcg ctatcaaatt agcctctgag ctaatagtca catattcata agcctgcaat
    27601 tccagttgct taataaaagc tcgttcgagt tctgcctcac tttgatagtt tgtcgcgcga
    27661 acactttccg gtgtgtactc ggaaacaaca gtgctctgcg tattcttagc gactagattg
    27721 tatttgttat taattggcat attctttgaa acttaacaat ttatttcgat agtattcata
    27781 ttgagatctt ctagcggcaa gctcagcagg taaacctatt gagatgtcgt tcactaattt
    27841 ttcaaactta tcaagaataa taacgatatt ttcctgttgg gttatggtag gaataggaag
    27901 taataacgat ttaactatct cagcatttaa attttgtaca gttccattcg atgctttttg
    27961 cgtcattgtt tgatagacgg cgtctgagct taaaaggtgg tataaaaaat ctgatatata
    28021 tgttttgttg aaatcactta tggataacca accgtcatgt atgcacccat cgatttttaa
    28081 aatatatggt cgaccaaaac tcattgagtt tgataataca aaatcacctt ttttaacaaa
    28141 tctcgatttc tgagcacctt ctggtttaat tttttgcaaa gtttgcgtta tatatttact
    28201 tcccttttca acatcaccaa tttttatcca gttgataccg ttttcttcct tagtaaaata
    28261 attattaatt ggccgtggag aaccacccct gacaatcttt gcactttctc caaacttcgc
    28321 ccactttatg tctttgccaa actccaagaa ctttgaacga tagtgattgt attgcttctt
    28381 ccttgcttcc agctccgctt ccagctccgc ttccagctct gtgaaattat taagaatttt
    28441 gactatttca ttttgtattg caagcggtgg caatgggatt ttaaatttgg aatactggga
    28501 aatccaatga cgagcatggt ctgttggagt gtagttaata cacttcattg cgtaatacaa
    28561 aaatctaaaa ttcacaccag cgttttgttt cggtcttaac attttcattg ctgaagactt
    28621 aactttaaat ttaaaatcaa cccaatgaaa tgaagtagta aaatcatcaa agataattac
    28681 aggcttatct ttacttgctt cgtatattcc ttctgtttcg tctgtgtagc cgaggacaaa
    28741 gctttgtcct gcagtcaaaa ctggagttgc gaaatcattg ctatattcta ttgaattaac
    28801 tatgtaatcc ccaggttgct catagtccaa gagatcatcc aattctttga aatcaacccc
    28861 tttggggcac aactgtgtaa ttaatttttc tattttgcct tgtttgttag tcatactttt
    28921 atgttgaacc ttcgatatcg tttacaatct cgtcgatggc tgttcggagt tcgttttgtc
    28981 tcgccacaat ctgggttatc tttgcattaa gcttagttat atccactgcc tcacgagtat
    29041 cttctgctac cacataactt gacaccgcaa cgttgtaatc gttatcagca atggttttgt
    29101 tgtctataag tttggcaaaa tactcagagt cttgacggtc acaaaatgca ttcaaaattt
    29161 ttagacgatt ggcctcgcta agtttgtttt tatttccacc cctcacaaac tctgcagaag
    29221 catcaataaa gagagtctta ttgtcctttt tgctcttttt gagaacaatg atgcatgttg
    29281 ctatacctgt tccaaaaaaa agatctgggg gtagctgaat aacagtgtct acgtagttgt
    29341 tatcgataag gtatttacga atcttttgtt ctgcaccgtt acgatagaga actcctggaa
    29401 attcaacaat cgctgccgtg ccgcttgttg agagccatga aagcatgtgc atggtaaaag
    29461 caagatcggc tttgcttttc ggggccaaaa cacccgctgg agagtagcgt ggatcgttta
    29521 taagaagtgg attagcgtca ccatcccatt ttattgaata gggtggatta gaaactatcg
    29581 catcaaaggg ttcttcgtcc caatgttttg gatcagtgag agtatcgcca agggcaatgt
    29641 caaagttgtt gtagttaatg tcgtgcaaaa acatattgat acggcagagg ttgtaggtag
    29701 ttaggttgat ttcttgtcca aaaaaacctt gacgcacatt ttctcttcca agaaccttgg
    29761 caaatttgag aagaagcgac ccagaaccac aagccggatc atatacttta ttaacttctt
    29821 tcttgcctac tgtagtaatc tcagcaagca gctcactaac ttcttgtgga gtataaaatt
    29881 caccaccaga tttgcctgca ttcgaggcat acatggtcat cagaaactca tatgcatccc
    29941 cgaaagcatc aatggtgttg tcggaataat ttccaaggcc aagattacca attgcctcca
    30001 aaagtttaac gagtttctga ttcctttttt ctacagtatt gcctaatttg ttggaattca
    30061 cgtcaagatc gtcaaacagt cccttaagat catcctcgct gcttgcgcct tttgctgagt
    30121 tttctatctt attaaaaaca gttgataaag tctcgttgag atttgcatca ttgcgtgcat
    30181 ttttacaaac atttgtaaaa agctcgctcg gcaaaatata gaaccctttt tccttaacag
    30241 tatctgtccg accaaattct gcctctttat ccgatagatt tgcgtaatca aaaccttttt
    30301 tgtctgcacg acgttcatca tcgttaatgt agttagctaa attctcgctg ataaaacgat
    30361 aaaaaagcat tcccagtaca tacgatttaa aatcccagcc atcaacgctt cctcttaaat
    30421 cattagcaat ttgccaaata gcacgatgca actctgtacg ttcttgttct ttggttctat
    30481 cttgagaatc tgtttttttc ataattattt tccattttta tgactaataa atttttcgat
    30541 atcttccttt ttgtatctgc gaaccttttt aatcccaatt cgtatggctt ttagtacacc
    30601 tttcttgtcc cataatctaa gagtgttagg gtgtactttt agtatctgag ctgcttcact
    30661 tagtgttaat agttcttcca tatgatttac ctaccaattt atactaatag catacaaaaa
    30721 aaactgatat ttttcaacag ttgtcaccag aaacgagcta gcatttgaca ctttaatttt
    30781 ataacctgag attgtaacta atttgcctaa cgaacctgtc ggggcttagg tttgtctatt
    30841 tcaaccgagt agatgaacct aagccccttt ttgttatcca catatgtcag agatcaaaga
    30901 agaaaaaata actcaggata gacactacga gcccacgcca gaagaagaaa agcttctaac
    30961 taagtggaaa gcgagattcc gacgtgctaa agaatttcgt gacccgtatc aagcgaaatg
    31021 gctcaggatg tatcagctct atcgtgccta tcagaataag caaaattacg cttacaacac
    31081 gcgcttaatg ccccccattg cctttgagat agtgaagact gtagtttccc gactggctac
    31141 tgccaaacgg aaaacacaaa tcattccgcg ccacagagat gacgttgaat ctccgtcgct
    31201 gggatcttgg gatgacctgg ttaactatga ctttgacatt attgacctga tcaagaagct
    31261 tccaagttgg atcgaatcat cggtcctgta cggtaacggt gtggttaagc ttgcttggaa
    31321 agtaattacc aggaagaaga aaactgtata cgacgaccca accatgggtc tgattgacct
    31381 ttgggaattg ctcccggcgc cggaaactat tgatgacctt caggacggtt gcccgtggct
    31441 cattcaacgc attgttaaag gaaaagagaa aatagaaaag gaagaagaaa gcaggggtga
    31501 tgataagatt tataagaatt tggagttttg tgagccaaaa atagttgaag actggaaaaa
    31561 ggaaagatac gagattaaca gtaagaagat gtctcaggta gatggtggaa ctaaaaagga
    31621 agctgatggg gaaaaggttc ttccggcaaa actcgataag gaaaaacaac ttgaactctg
    31681 ggagtgttgg gattatgagg aagataagct ggtagtgatc tgcaatggtg aggtggtaat
    31741 aagagatgac gataatccct atcttgaagt caacaatggt catatctatg ttgatttacc
    31801 agatatgccc ctgctatggg aattctgggc gaccggtcat atcgaacccg tagaaacgac
    31861 aatccatgaa attgcggatc tgagaaatca gcgtatggat gatgtcatcc tgatgttgga
    31921 cccggtagtc aaaattcgta aggattctgg aattaccaaa aacgatatta tcttttctcc
    31981 aggtgcgact tgggagttga gaaagatgga tgatgtggtt atcgaacggc caccagaaat
    32041 aagcctcatg ggtgttaatg aagataaatt aatgagggat gaaatttctc gcactcttgc
    32101 ccttggcgaa tatatgagtg gccaaccaca atctcccgat gaaccccttg gaaaagtggc
    32161 aacacttatt ggccaaagta atctacggct tagcatgaat gctcaaaata tcgctgttgc
    32221 cttgacccag gtggccaaca ttctcattca acttaatcaa gagtttataa gcgaggacaa
    32281 gctatatcga ttaataggta aggatgtaga tttcaaagaa ttcaagaagg aagataaaaa
    32341 agtagtagtt gatgcgattg ttgaaattga gccgataatc cccccagata aaactacaaa
    32401 actaaatcag gtcctactca tgtatgacaa gttcattgct caggacaagc cggatccaaa
    32461 taatcccgag gaggtaaggc gatggttgaa acgtaagcga gaattgcaaa agatgatttt
    32521 ggaagagtta gataaagaag aatacataga gcttttgttg ggtcccgatt cagaagccaa
    32581 ggagctacca caaaataatg atgcacaagc ccccataaca cccgctgagg ggcaaactcc
    32641 accagcagag ccaactacac ccggtccaaa caaaattcaa caacttatgg gtaagatacc
    32701 aattttaggg aagatgttgg gaggttaatt tgctcccttg aggaaaagaa ttattggacg
    32761 tgagaaaact gttaatgctg ccccaatact aagcatgtgt tcaggagtcg gcacttttgt
    32821 atttaatgcc ccttgtaaaa tggttgtctc agaagggacg agctcgtccc caaacattgg
    32881 gcggacagtc attacctttt tcaaatctac ctctgataat gaattaagtc ttctctcgca
    32941 aagtctcacc gaatcggcaa aagccatgct cgaacgtgtc tttgatgcga aagaacggaa
    33001 cccggaaatg gggccaactc ttaatcgtcc acatatattg atgactttat ttatagaacc
    33061 cttacgttca ataaaagcat taagtacggc acttccacca gcacttgttc caactaaaga
    33121 aactgagtct ccattttccg taaacccatc aatcatctca ataagtctat gtagttttgg
    33181 ttcaaatgaa ttttctccat cacgccaccc aacggaataa actactggtt tcaggccgta
    33241 gttcttccag cttcttattg cccattcaag ttttttagta tcatcaccta gacctggaat
    33301 aacaatgact ttatgttctt ttggcataga tgacaaaaat tagtttactg gcttatatat
    33361 ctaaattatt tttatccgct aatttgttta gcatattctt catcaaatac attactgacc
    33421 tggtcaattc ggaatccctg ccctgtttta tcccaatcaa ttggtaacgt tttgtatcca
    33481 ggtgggataa agtcgtttaa cttttggata tcacttggat ccaatttgtg tccaattgct
    33541 gccaggtgtt cgtcgatatg tgctttattt tcagacttgg taagaggtaa taagccttta
    33601 gatataatcc aactgaggac cacttggttt tgagttattc cgtacttttt ggaaagctcg
    33661 actaaaattg gccaattcct gtttgcggtt cggttccttc ttaaaggctg ataaatggca
    33721 gtcacgattc cattattctg ggcgtactca atggttcctt ccgcctcatt agcacgaact
    33781 tcaaagttga aacacacttc atgggagaat aatttgtcgc caaatctttg atgatatttt
    33841 ttgagtagtt ctaagttttc attggtaata cttgtatatc tggatttacc ggcagttaca
    33901 accttatcgg ccatactgca aacctcatca aattcataat ctaggaaagc actttgagtg
    33961 tactgaaggg tatcaatata gtcagtatcg aatattttta ataatctatc gatttctgct
    34021 tcggtatctt ggagtttgtt atccttcaga taaactgcct gaacaataaa tatctcctca
    34081 cggtttttac ctgactttct gaaagcttcg gcaagaatct cagcagagta accctgagaa
    34141 taccaaagat tggcttcgac ataattcatg cctttttgaa acatgtaggc aatagcgtca
    34201 acttgtttat tcttatctag ggtctgatct ttttctgcaa atccaccaac accccaggtt
    34261 cctataccaa ttggaaataa atcatttttg gttatcataa tttctatacc accacgtttt
    34321 gtggagctcc atttataaat gccttaacat tggcaatagc taaagtgtac gacttagtat
    34381 tggcgtcttc ggtgttgaag gcattgtgtg gagtaaccac aatacggtga tgtttcaaat
    34441 aaggactatc cgtaggaact ggcttcataa tttcagtctc aattccaaaa ccaaagattt
    34501 tgccactctc aagtcccttc agaacagcct ctttatttgt aattggctcc attgcaggat
    34561 taatcaagat gacaccatct ttcatgagag ttacttcttt ttcagagatc atatctgtgg
    34621 tttcttctgt tagtggaact gtggtgacaa taacgtcgct ttctttgaga agtgtttcca
    34681 aatcgacttg cttgatgcct tcgattgtct tttggctacg gttataacca agaattcgca
    34741 tgtcaaagcc agaggcaatt cgtgccactt tggttccaat atcacctaga ccaatgattc
    34801 cgatcgtttt tccatatacc tctttaccca catagtcagc aaacttaaat gctcctttat
    34861 ttcgaacgtc gcgatcgaac tcagtaatcc gctttgcaat gtctaggatt aaaccccaag
    34921 catgctctgc aactgattca gcattagcac ccttgatgtt gcaaatcgta atgcctagtt
    34981 ctttagctgc ttcaatgtct acccaagcaa caccgactgt tagagtggct atcattttca
    35041 agtgcttcat tccagacaac aactctcttg atattttttc aataccagag gaaccggcaa
    35101 tcaaaatctc tgcatctcct gccctggata taacctcatc tgcggtcatt ttttgagcgt
    35161 ttatgacaat caattcacct aaactcttta actcaccctc cattgcccca gaaattattg
    35221 gattagatgt gattaagtaa attttcatat taaacgcctc gtctttgatc aactagacca
    35281 cccatataat tagctttgta tatcaacttt tcaccttggg taatttgttc ttctcctgaa
    35341 tatctttcaa cgtctcctgt ccaagaattg cgatatgtaa gttcaccaac aactagctct
    35401 tttggtccac ggaaaggagc ctcttctggc atagctttta aagcacctct caaagtggcg
    35461 tagactggat cagtctccac accttcggct acccacccgt agtaaaccat catccaaatt
    35521 ggtttatctt cattaaagac gacgactcta ccaccataag gttcaccacc aaagaagtta
    35581 tcatgagatc gccatttgcc ttgagtataa ggaattgtgg tagatccatc tggctctttt
    35641 atccactttt tctcttcacc accagcgtaa ccggctttat ttgaatcgat taaaaactgt
    35701 cttaaagttt ctttattcat aggtgctcct gattacaatt gtacatttct ttaatttctt
    35761 acattgtagc aaaactccca cctctttacc aaccattttc tcggtataaa caccagaaac
    35821 ggacagaaag attattgaag atatccgcca tctttgttta ctattaaacc attaggaagt
    35881 tagcctaacg aaactatcgg ggctgtggct tctgaatata cgcaaatagc gtgtgtttag
    35941 aagttacagc ccctttttat ttacaaagct atgcaaaaaa ctgaacccga attaaataat
    36001 aaaaatcaga gcgtgccatc cctcgatgca aataacgacc agaattcgac cactcaagat
    36061 caagaccaaa agcaaattgt ctggggaaaa gtcgatgtgg tgtacaacgt ggctaaagaa
    36121 gcgtttcttc agggaacttc atccattgac gatgtgttgt ccagtctaat tgccacatta
    36181 caggagataa agaatgacga gattcaaaac ttagggggtt tgggaacagt tccagcaaac
    36241 ggtaatttat taggtaatga gttagatcaa caaccaccag aacaaccatg acaaacacac
    36301 cagaggaaaa ttttgaacca ggacgagaaa tatgggaaat gacccagaca gcaggttggc
    36361 aaattctttg tcagcaaata gcgcaagaaa ttgagctaga gaccaatgaa ttgcttgatt
    36421 gtccactgtc tgaggatgtt gaacacaaac aaatgattaa agcatataaa aaagttctaa
    36481 gcattgttga aagttctttg aataaacgta acgaaaatac tcaggttaat acaaaaattt
    36541 aaaggaaaaa tatggacgaa aataaactaa aagaacaaga agagcttaaa aacctactta
    36601 attcagacga tactgctgat actccagatg ggggtggtga tgaggataaa aacaaaccgg
    36661 acgattcaaa caatgataac gaggatgata agaacgatcc cgataagaaa actttgaaag
    36721 atgatcctga taaggatgct acgagcactg acaacaaaaa ggaaaataaa cccgctgatc
    36781 ggtggagtgg taaaagtcga gaggaagtaa ttaaagaatt tgagactctc gagactaaaa
    36841 cgactattcc aaataatgac aaaaaagatg atgacctgaa acttccccct caggaagaac
    36901 ttcaaaaaat gaccccaact gattttgcta agtgggtatt aaccagaata aatgaaggtg
    36961 tcaaacagac tcttgaaact caagaaaaag tacgaggtgc tgtccgtcgg gaaatcgatg
    37021 aagcaaaaaa gaatcatcca ttggacgatc ctgactatag aaaaatggta ttagccatta
    37081 ttgatgcggc gagtgcaaaa gggtcaaacg tacctttgaa agaagcgtgt gaacaggtgg
    37141 atgcattttt gggaaaacat actgggcaat ctgaagatat atcagatgac gagcgtagcc
    37201 gacttaaaaa ggctaaggct caagtcgaga gtggagcggg cgctccgact caacccgaga
    37261 actcggatgc tgaaactcaa cgcatccaga aagctcttgg acatggtggg tcaaaaggct
    37321 ccttaggagg gttgggatta tgaccttcct gtctaattat cattttataa ggaaaggggg
    37381 tgaataaaaa ttatgccatc aacaacagga attagaggaa caggaaatca aggagttacc
    37441 agaaagtatg atgtcgctga cgttgtttcg cttttggatg ttaatagata tccattgttg
    37501 gcgattttaa ctaatgctgg gaaagatccc gttactggtg agggtaaggc gatgaagaaa
    37561 aaagagtcga tggacccaga atttaaatgg tttgaagatg aattcggacg aagacagctt
    37621 acgggaacgt caacaattga ccctgacggt ggaaatctaa cgcttacgag ccaagctcaa
    37681 tacctccagg ttggagatat agtattggtc gcgaagcaaa agtgggtttt ccaagttagt
    37741 gcaattgtcg atgcaaacac tatttcggtg ggaccggaac ttggaggtgc gactggcgcc
    37801 gcggctgatg cttcaggaga tgtttggctt attggtaacg ccaatgaaga aggatctgga
    37861 ttgagagaaa ttagatctac agtcattcaa gaaatagcta actactgtca aattttcaga
    37921 actccagttg gtatcactga atcagcaaag aatactaagg gatggaccaa agaaagcgat
    37981 tttgactacc agagacggaa aaaaggaatt gaacacatgg tcgatattga acgttcattt
    38041 ctcttcggta aaaaaggaat tattactact ggctcacatc caaaacggtt tactggcggt
    38101 atcttagcta gaattgcaac atacgcttcg gcgagtgttg atactgaatc tgaattcgaa
    38161 gcatggttgg aaagtctatt tgcttttgga aacaccgaga agtatctttt tgcgtccgca
    38221 tctgtggtct caatgatcaa cagctgggca aaagggaaag tccagatagt taatccgaca
    38281 aaaacatatg gtcttcgtat cgtaacttat gattctccgc acggaacttt gcatattatc
    38341 aagcacccac tcttagcggg tgtgacttat ggtaactatg cggtcggatt ggatatggaa
    38401 gcgttgactt accgttactt aagtaatcgt gatacgaaac tattaactaa cagacaggac
    38461 cctggcgaag atagtcaggt agatgagtat ttaactgaat gtggtctgca aatggagcaa
    38521 gaaaaacgcc atgccatcat gagcatcggt gctctctaaa aatacagaaa cgatatgaaa
    38581 cagattagta aattgaaaaa aatgaagtca ctgcacactt tgttactcag tgcagtcgtt
    38641 taagccaagg gtgaatgtta tcacccttgc aaactttatt aaagatagga ggtgaaaaac
    38701 aaatgacaga aataaacgat aaaacattag aagaaatgtc catggcagag ctatggggtg
    38761 aagcaaaacg cttaggaata tcgaaagaag ggaaaaagga agacctgatt gctcgtatta
    38821 gagaggcgaa agtaggtggc gcatcatcag acagtaacga tgatcaagat caggaaagaa
    38881 aggtaagcaa ggaggttatc tatatctcga gatatcacga acttaaattg gtcgttgatc
    38941 catcttactt gaaagaggtt ggtgtccgag tgttgacaat taagggcaag tgcattcagt
    39001 tccatgaggg aatttacaga acctcagatc ctgaagaaat tgcttttctt aataaccatc
    39061 ctaattatgg caatgttttc cgcaaaatcg aacaggttga aattaaagga aaaaatcttg
    39121 agcaagttta taaggataaa tttaaaacgc ttgaagaaag agagcgggac attgctatta
    39181 gagaagaatc cataaaacgc aaagagatgg tgtcaaaagg tcaccaagaa ggtgcgtctt
    39241 cagttactag tggaattgaa gacacaacca atcagcctaa attttaaaag ctaaaaaaat
    39301 atggaaagca gacctaaatt tacaacggcc aatgtcacat tgaccaacgc cgacacttgg
    39361 tatcccctgc cagatattgc ggtcggcgaa ggatgtgacg tggtgattaa gtcgaagact
    39421 gggaatactg gtgcaataaa ggtcgcccat gatgatgcta ccagcaaagc atcgccgttt
    39481 ttactggata atcctggaga cagtgtgagc ttgagaatta agggaacgac tcaaatcgtt
    39541 ctctcttcca gtgtcgttgg acaagttgtg gaggttgttg ctgaacaata aatatggcca
    39601 aatttaaacc atcattagta aatattcgaa gaatgttctc gaagatgaga atcatcgtca
    39661 atactgatga tggcgaagaa gattacgaca caaccttcta tcccaacggt tttaccgcta
    39721 accaaatttc aatcggtaac gacaacactg gcagtaatga tgctggattg agattcttaa
    39781 gaccactact gcctaacaac gtcaaaataa ctaaagccat tctgcgagtc aagtcggccg
    39841 ttaattccac taagcgcccc atctgggtaa ttaaggggat taagcaagcc agtaccttga
    39901 gctttgattc cactcaccga ccatcacagc aacctaaaac gactaccaca gttgcctggg
    39961 tccctggagc ggattgggta attaataact ggtatgaaag tccagatatc aaaacgatta
    40021 tccaagaaat cattgaccaa acagaattta ccaataaagc gattggcttg gtgattgaag
    40081 acaatgccag tccagccaat aactacgaga acatctggga ctttaacagt ggagcggcta
    40141 acgctgctca gcttgtatta acacttgaac cagatttgta aggaggtgaa aaaaatatgc
    40201 aaacaaatga tgctgcgcta ttacaaacga ttttagaagc catagttgtc agtcccgaca
    40261 aagtggcagt tgagcgaaca gtcgacgaga tgggagtttt acttaaagtc aaagtcgatg
    40321 aaaaagatgc tggagcagtg attggaaaag aaggtgccac tattgctgcg atccgaaaat
    40381 tgatgggcgt tttcggaaga aaaaataacg ctcgaataaa catcaagtta gatgttccgc
    40441 ccagaaagaa attacaaaag tatgaaaaac caagtattga agatcaaaac cacgatacag
    40501 gaaaactctg atgatggccg agaggtgggc gtttcgactt ggtatgcaga tggtaatgcc
    40561 ggtaaggtaa tcaccttcgg caattttgac ggattaaacc aagcagcagc tcttcgtttt
    40621 cgcggagtta acatccccaa attcgccaaa gtcttatttg ccagacttag actaaaagtg
    40681 gcttcgactg atgatgttga ctttccggtg ttattaaaaa tcaaaggtat taaggaagct
    40741 gatcctcaac catttaaaca agtaagtaga ccgtcaacac gcactgcgac aatcaatggt
    40801 gtcgactggg atattctcaa aaaatggaac gccaatgagt gggttcaaac tccaaatttg
    40861 aaactaatca ttgacgagct tgtccaacaa gctgactgga aaccaggcaa cgctatggcg
    40921 tttgtaatca gcgatgacgg cagcacaacc gggaatgcta aaacctgctg ggattcaagt
    40981 aaaggtaaag gttacgaagc ggagctggaa gtttattaca ttactccagg tatccatgaa
    41041 gaaatctcaa tcattagttt atccggtaac gaccgtgatg gtgaagaaga ttatgacacc
    41101 acctggtttt ctagtggcta tgccagtaat cgtataagca ttggcaatga cagtacaggt
    41161 agctgtgatg ctgggctgat attcagtccg gtcaacattc ctcaaggcgc tgaaatactt
    41221 tcggctcgat tactcctaac gagcgaagcc caatggaatg cgatgctgaa cctgctgatt
    41281 aaaggctttg ccgaagacaa tgctccagta tttgcctcaa atggctcaaa ccgcccctct
    41341 actcgaacta aaacaacaac ccaagcccaa tggacattgg gatttggtca gggtggagtt
    41401 ttggtgggtg aacattgggc tgcccaaagt gtctatgaat cgccagagtt aaaagaaata
    41461 attcaagaaa ttcttgatcg accagattgg gcaaaaaata gattagggtt ggtgattgag
    41521 gattatgact cccaagctgg agcggttaaa aacattttcg attcaaatca agatggtggt
    41581 aaatatggag caaaacttgt tattgcttgg acttgtgagc gacgagtgac atcaagagat
    41641 aaagacacta agggttatga caaagcaaat tacccagaat atattgtggt ccaccactct
    41701 gcaactcctc gggataatac ccactttgcc accataaaaa aatcacatat tggttatggc
    41761 tgggatgaca tcggctatca caaatggatt gcaggaggac ttgatggtct agggactttg
    41821 attcctggcc gaccagaaaa caaaatcggc tcccataacg acagcaacaa aatgaactac
    41881 cgttccattg gtatttgtgt ctgtggtagc ttccactcga ccggtggtaa cgagcaacca
    41941 tcagccgagc agttggccac cttacagaca cttctggatg atatccggat gaagcgcagg
    42001 attcccaaag aacgagtcat gggacacggt gagacaccgg aatcgacgac tgattgccct
    42061 ggtgatcact tactggctta tatcaaaaat taccggaaaa ccgggtcatt aacttaaaaa
    42121 tatgcaatta caagaattta gacaagatct cgatgccaga atgtcggccg ctaaaataaa
    42181 cggattctgg aagcagccgg acaaagatcg ctggattaac aaaggtattg tccgggcgtg
    42241 taactatgcc cgttggaaat tcttgagtaa acatgccact caactggttg agaaaaaccc
    42301 taacggaact ctcaaagaga attattttct accgtttgac ttcaaaccag gtggattcat
    42361 aacaatcaaa gttggtggaa ttccccatca taaaatcagt gaagacgaat atacccaaca
    42421 gacctattcc tatgagcgag tggtggccat tattggtgac gagtatcacc ttaatttaag
    42481 tgaactcacg gatgttgctg aaggagcgat catcgacatt tattacaaac gacgtccagt
    42541 gagattggtc gaggatgatg acgagccaat tactcctgaa gaaatggatg aaccggtcgt
    42601 caagttagca ctttccgttt gtttaaccaa aacccctggt aaacaatctg actcccaaaa
    42661 agaagtagta gaggctcata ccatcctgca acaaattaaa gatagagagg atgaggaacc
    42721 aaccagtgtc tataaaggac aagctggtag ttcccggtgg gaataaaaac tatggcatta
    42781 aacgcatata aaatacaagg ttttcgagga gggatcgccg acgaccccta tcaaggagtg
    42841 cgtgaagcat tccgatttgg ctatggctta aatatccgtg atgacggcaa cacccttaaa
    42901 tgcaaccaag ctctcaaaga agatctaggt agtttggaag taataacgga tttgattctg
    42961 ttttatgtcc cggcatccaa cggcaaactc tatggctttg gcgactctgg aaatatttat
    43021 tgtaaagaca cggtcgatga tgcctgggcc ctggtttatc acgatgctaa tggaaaaata
    43081 accggagctg ctgagtacac caataatgat ggttctgata actacattcc ctacctctac
    43141 tgggcaacag aaaccaaagt cagtcgaatc aaactctctg gcacttggac tactgatgtt
    43201 gagcatgact ggggtgcctt aaacggtgat cccgcttggc acacgatggt cgaagctcta
    43261 ggagtgcttc ttatttgcga tgctaaagat ctggccatgg ttgattacga aggagccttt
    43321 gccggtggta tctctccagc acttgatctg cctaaaggac atcgtaataa atgtttgctt
    43381 ggacaagatc agttggtgat ttttggctca actaaaggtg acaaagtcga agaaggctgg
    43441 ctctggactt gggacaaaat tcaaccctct tggattcaac gccgaatggt ggcagaacgc
    43501 ggtatcaacg ccatgattca aggcgaatat cttctaatac aagctggggt ctatggtggg
    43561 ctctatttct gggataccgc caccttgatg cgaatcaaaa agttccccgg agaaaatggc
    43621 tgggttaatc cgggagccgt ttgtaacatg catgggctcc ccttattcgg gctgaatggc
    43681 tcagataaat gtggcgtcta ttcatacggt cgactgagta aaaacgatcc ctatgctctc
    43741 aatctagaat acgttccttc gcatggcaag ttggcagatg tctcgatcgg tgccttgact
    43801 atgtacaccg accaattgtt tgtttcctgg aaagatggag ctacgtttgg cgtcgatacc
    43861 atcgatgtaa ctaaaaaagc tgaaggtcgg tatgaaggct tggtatttga tgccaatgag
    43921 atctttactc aaaaaggttt tcgtcacatt aagctcgtca ctaaatcgct tcccaaagat
    43981 tcaagtatcg aagtgtttta taaagtaaac gaacaaactg actggcaaaa agccaccatg
    44041 gaaaacggca atgaagcctt tgatcaggaa ggtaaaacca aagccatatt tacgattgaa
    44101 accggtggag acgaagacga accagatcga ggtgaggaat atgaagtggc actcaacatt
    44161 caccccgctg ataacgaaac acccgacatc aaatcaatta ttacctattt tgaaccgatg
    44221 ggaattttat aactatgttg actgatttaa tacaaaaagt atttggaaat aaggaggtta
    44281 ccgatactcc gtttccagtc gtgccaactc acactcataa cagcattgac tcaccagccc
    44341 tagctcctgg atctgttagt acagtcaata ttaaacctaa tgcagttgat gaaagtaagc
    44401 tcatggattt atcggtaact gaggaaaagt tggctgaagc agctgtcgcc acccaaaaaa
    44461 taaaagatga tgcgattact gccgccaaag tctggaaagg agggtcagta attaccctct
    44521 ctgcccaaat tgcagaagcg attatcttaa cggcacatat tcaagactta gcggtgaaat
    44581 gggccaagat tggaaatctt gaggttggca atagcaaaat tatggatgca gcaataagca
    44641 atgccaagat tcaggactta tcagtcacca atggcaaaat cggacttttg gaagttggta
    44701 actctcgaat aatgaatgct gccatcagta ccgcaaaaat acaagaattg gccgttgagt
    44761 gggctcagat tgcaacgctc gcagttggca actcaaaaat tatggatgcc gctattagta
    44821 cggccaagat tcaaaactta gctgtcactg atgctgaagt aaatgatttg aatgcagcca
    44881 aaatatcggc aggactgatg accgctgaca ggatttgggg tggtgctggg tattttgatt
    44941 acatccaagt tcgtgaatgt ggtactcaag cggttaatgt tgctggaaat attaataaaa
    45001 caggaacctg caactttgct atccctcacc cactgaaacc tggcaagcag ttagtttata
    45061 ccggaattga atcggccgag gtaatgctcg tcgagcgcgg gagtagccaa ctccaaaacg
    45121 gtcaaaaacg aattgatttc tctgatcact acaaagcagt cagtgatccc agaaaagcga
    45181 cagcctatct cacccctcga gaagattgca aaggattact actcctagaa accgctgaag
    45241 accacatcac tgttaaagaa atgagtaatg gtagatcaaa cgccaaattt gattacatta
    45301 tttttacggt ccgtaaaggt ttctatggat ttgattttga accagaggat ctctcacttc
    45361 tccaaaatga cggtgaagat gaagaatcgt tcaaacaacg ctatcacgat caccggatca
    45421 aattaattcg taaaaaaggc ggtccagata ttgataccaa agtcgctgaa tttcaccaag
    45481 cgtggatctc aactcaagga aataaaaaag ttaatcaaaa ggaggtgaac acataatggc
    45541 aacaaaacaa ttaggcggtt ggtattggca accagaacaa aataaagctc tccggtggtg
    45601 gggtactgac tctgctggaa aagatattta taccgaaggc gacgaaccaa gtaagcccca
    45661 aactgtatcc aatttaagca cggggttaag tacaatcgga tccggaaacg ttttgggctt
    45721 tgacatggga actctcaaca ctaaatttca agatacctat ggccagattc aagggctatc
    45781 gaccgaacta gaagactact caacccgtcg atatgatgag gaatacagca aggcaggctt
    45841 gagtaaaact aaagatgaaa ttgcagcatt agacgctagt atcgccagtg agaaaaatgt
    45901 ccgagatgaa tcagtaagca aggttaggaa aaatcctggc tactcagctg caaccattac
    45961 gggcgaaact ggagaggtcc aaaaactggc tggctctaaa attggtaatt taattgaaga
    46021 gcgaaacgcc aaagcccaaa cctacaatgc gacactgggt gaaatcaccc aaaaagtagc
    46081 gacggaaact caaggtaaac aaagtaagct caacaacttg cgttctgatt tgcagcttat
    46141 aagcggactg gttgattcct acaacaaaac cagatcgacc gagctttcga gtcaaaccga
    46201 tgaaacacat tgggaaaaag agtttgaact caaactctat ggtgcccaaa ccgatcgaat
    46261 gaaaactggt gatggtggtg gcacgtacgc taaacaacag gttaaggatg cctttggtaa
    46321 tgtgactggt ttttttgatc cagtatcagg ccaaacaaca ccttatcaaa ctgattctca
    46381 aaaacaacaa accacgaccg taaacgaagg cgaactgcga aatgaaattc gatcagcctg
    46441 gaaaactggg ctatcagccg atcaactaaa aacgaatctg gccaacgtca cgaccgataa
    46501 aggcaaaaac gcctcacagg tcattgatga agaatggcag ttaaaaacac aaccgggagt
    46561 tatgggcttt ctaagaaggc tctttactcc gggggtttaa atttatggac aactttttag
    46621 aacaactaaa gaaaaacatt acctcgtcgt ttgccaatac caaacagtca gtgggtgata
    46681 tttttggtaa ggctcaaaca agtctgacta gtctggtgcg tggtggtcaa caaaaagttg
    46741 atgaggttat taaaccactg caatacgata tcgcaatctc tggcccaaca agtggaagct
    46801 acaaaccgtt caagaattta tatttggaga cggcagccca agcgttagga gttggagctc
    46861 gggacactga tgttttggag agattgccca gtgcaacttt tggtgcactt aagggtgctg
    46921 gcaatggatt aggtgaagcg ttgtcgtttg gttggtggaa accagaaata aagtatgccg
    46981 atgatataga aaaacaagct ggtgacatga ctgagattga gttcaacgtc ttgggaacca
    47041 ttggaacgtt tttggttggt ggcgaattgg tccaaggagc tttgcgcggt gtgccccttc
    47101 tggcacgggt agcaactgtc gctcctagaa ctttcaatgt gttatctact gggctgacgt
    47161 ttgctggact cactcaaatt caaaaagatg aagcgcaaaa gaatgcatta cagcgagcaa
    47221 aagattttat gggtgatttt gctctcggtg gagccttctc aattgccgga atgaaaacat
    47281 catttctgaa gtcatcagcc attatcggtc cagcaactta tgtcacctca cttctaaaag
    47341 gagagtcaaa cggtgatgcg cttaaagata cagcagccat gatggggctc cactccctta
    47401 atttcgcggt ggctaagttt ctacctaatg gatcagaaat agaaaatcga atagaaaaag
    47461 cggccagtga gcagctcaaa ataacaaagg ctgaagccat ggagtatctg ggcattagtg
    47521 acaaagctac tgctcaggag atgaaacaag cctggaagaa taaggtggtt gatattacta
    47581 agcgcttccc agctcaggcc aatcaaacac cacaagaaat gggagtattt aatcaagctt
    47641 ggaatactgc caacagagcc tatgagtttt tagctaaagt cagtaaccca gagggttata
    47701 cagggaaatc attcgctcaa gagtttcaag atctatatta ccaactctgg agagatgttc
    47761 ccgataagcg ttctgcgatt gtcaaagccg tccgtagttt acctgcggga ctgtcagttc
    47821 gggctactct taatgatggc cagcgtaatg aaattgtttc ggcgcttggt ggtgaagggg
    47881 cacaaacgag actggggaaa gtggcggaac tgactgatgc agatttggtg tcagtggcca
    47941 tggaaaacaa aataaaagtg actccttcaa aacccgcagt cactgaagag ttggatataa
    48001 gcactgaaac atataagcat ggcacacctg tgccagataa aattttgaag gaaggtttta
    48061 gtttagtaaa aacaggtgaa acgagcggtt atggaggtgt tatgggtaga ggtatttacc
    48121 ttgatctcac tcccgaaggc gaaggggcaa aactgtacgg tacagttgtt gatgcaaaga
    48181 ttaaaaaggg gctgaattta ttcgagtggt caggaaaact tgatgaattg tacacagaag
    48241 ctacaaagta tggagatcca gaaacaataa ccgatgtttt acttaagaag ggatatgacg
    48301 gagtaaaggg attgaatcaa atggtgattt ttgacccaaa aaatgtcgta ccagttgtag
    48361 acggaaaacc tactgtcacc tcaaaaatat ctttgtcacc acaagaacaa aaagctgtca
    48421 aagcggccga gatccgagat aaatatataa caccagaaga aaaagaattg gcagatgcgg
    48481 aaaagattcc tagtatcggg gacattctcc agtcatcaag ctttcaggac agattacaag
    48541 gaaaagtaat tgcgccgacc accccgagag ctgatcaatt gagagaacca ccttccggta
    48601 aaccgcctcg ttcacctcag gagatctcct taaacgacgc cccaccttca cctgaaggca
    48661 gtgccttgga tgagatcgat aaaatggttg gcaagatctc aaccgcaaag tcgattctgg
    48721 gaaatgtaaa ggatgcaatt caatctttcc cacgaatgtt tactgaccgc tttgctccca
    48781 ttaaacaatt tgaggatcaa attaccaaac ttcaaggaca gccaattgat attgattcca
    48841 gtccatatgt ggcagccaga atgtatgctg gtcgctttgg aattgttgag ggaagcttca
    48901 aagatttata taacatcata cagccggttc gaaagcttcg agctgacttt actcgatttg
    48961 ttttgtccga acgcgctatt gaacgaggag agcgagggtt tgataaccca aatggagtta
    49021 caaaggaaac tgcagagcaa gcactcgtcg agctaaaaga taaagtaggt gacaaggcct
    49081 tcaaagcctt cgagcaggtc ggccagggaa ttcaaaattg gtccattaaa tcaattctcc
    49141 agccgatgaa agatgccgga atcctgggta aagaagcttt tacttcaatt attgagaaca
    49201 ataaaaaatg gatgccattc catgttttgg aatatatgcc ctcaattgag caagccgatc
    49261 acatggcggt aggaagcgaa acgttttcag taagtaaaca aggcatcgtt aaatctctag
    49321 aaggaactga gaaaatcatc cgtgatccat ttatgtcgat cgtggataac ctgacaaagt
    49381 cggtaagtct gatagaacgt aataaagtag cccagaaact cattgaactg agaaatagcc
    49441 atccagatac agctaaagag tttataaagt ttttaccgga tggtctgcga ccaccaccag
    49501 aatgggaagc gattagtgtc tttattgatg gcaaagtaac caagtgggcg gttcccaaag
    49561 atctctcaga agccatgcat gccctgtcac cggctgaaac cggtttaatg ggtaaaatga
    49621 tcctagcttc ttcgaaagtc ttcaaagccg gaaccacctc actctatttc ccttttactc
    49681 tttcaaacgc cattcgggat taccaaactg ccacaatcgt ttccaaatgg ggtttcaatc
    49741 cggccaactg gctgtcggga tttaaggatg gtctaaaagc tgcctttaag tttgactcca
    49801 aagcctacga cgaatttatg actaacaaag gtggttatgg tggttacatc gaatcagcca
    49861 aggggctagc tgttgcctct gaacaacttt tcacccctga gtggatgaat cgagcaaaaa
    49921 ttgtcgtcaa tccatttcaa ctgattagta ccttctcgga agcgatcgag ctggctccac
    49981 gactcggagt ttataaaaaa ggaatcgctc agggtgcaac ccaactagaa gctgcctatg
    50041 aagctcggaa tgtgacggta gattttgcta aagccggaat tgagggtcga ctcatcaata
    50101 tgtggattcc tttcgtgaat gcccgttggc aagggcttct caccatcggc cgagtaatga
    50161 aagagaatcc aatcagaacg acggctcgtg ctttggcatt gacggtccta cctggtgtgg
    50221 ctacttactt ttataacgtc atgacccatg aagatctttg ggacgatatt cctcaatggg
    50281 ctaaagatac ttattttatt atgatcgtag gtgaaggcca ggatagtcag ggcaatcggg
    50341 taccaaaagt ggttcagatt cccaagggcg atatgggaac gattttcttc aacccgttaa
    50401 tgtatgcgtt ggagtatgtc cgaaaacaag agccaaccaa cctcttcaaa ctaggtctgg
    50461 agtggatgag ccaattatcg ccggttgcgt ttaccagaga tggagaattg tcggctcagt
    50521 ccttccttgg tagcgccctg cctccagcat taaaggtgcc gttggaacta gcgacaaaca
    50581 ctaatctttt caccggattc ccaattgtcc cacaacgtct cgagaaagtg gctccaacgg
    50641 aacaatatga cgaaaaaacg ccacaagtcg ccgttacggt tggacgagct ttgggcatca
    50701 gcccgatgaa gctgacccat gctatttccg gacttgccgg aagcttctct cgccaagtga
    50761 tgaacccggc cgatatcatc aacctcacca ctcagcgctt ctatcggctt cagggtgggc
    50821 aaaaacaaca agctgcctgg gatattaagt acgatgccga agtcggttat aacaccacac
    50881 ggctacagat gaaacgattg atcgagcaag gcaagctgca ggaagctcaa cagatggccc
    50941 tcaaatggaa ccaggaggca ggaaagctca ttcccgaggt tgtaccactc ttaatgaaag
    51001 atgaccccaa ggaagcggcg ttattccaaa gtagcatcac ctttgatgct gcagatttac
    51061 agcgactctt aaaaacaact ctccctggtg gagttccgga aaatactgga actgatcaaa
    51121 cactaatccc gcttcaacct ggacttaata ctaattcgac catgggacaa ctattccagg
    51181 gtggtcaaca aaatagtggt gacactataa ggaggttaat ttacaaatga aacaactaac
    51241 actgcccgta acttttagtc aaaacgacag tcgatggaaa actagtcctt taggaaccag
    51301 aggcacgatt ggctcatatg gttgcttgga gaacgatgca actatggtag ccaattatta
    51361 tggctttcaa gagactccac ttaccttaaa cgagaaactg accaacaatg ccggatatca
    51421 ggatggcaat aaattcgtct ggagtgtatt tgctcgatta tttggtctga agtattctgg
    51481 tcaatggtcc aatgcgggag cactaactca aacccagatg gatcaaatca gaaaagccat
    51541 tgataatgga tatcccgttt ttctacagat cgacacaatt cctttaacat ctgcccttga
    51601 tgaacactgg atcctagcaa ttggctatga gggtgacgac tttctggttc aggatccttg
    51661 ggatggagcg actaaacgaa tcaccagctg gggtgtcact cctcaaaaac tgatctatgc
    51721 ctggtgttgg tttgaaggga aaccagctca ggtcgatgtg gccgtaggat gtctggtccc
    51781 aaatactccg gagtggcgta ccaagtatga gcaaattgtg gctttagcta ccaagtgggc
    51841 tgataccctt aaggttttgg agattaccga cgaccctaac actaccccta ccgacaaaat
    51901 taagagcatc ctggcagggt ataaaagtcg tgaaactgac ctatctaaca agctaaatga
    51961 taaacagggc ttgctcgaca atgccaataa ggagattgag aacagaaccg aacaggtaag
    52021 cagactcgag ggtgatctgc tagagaagga taaatactat aaatccctag ttgacgcact
    52081 aaataagcag gtcaaaaacg gctcagatgc cctacccttg gctcaggcta gagttggggt
    52141 gcttgagggg gaactggatg aagccaataa agccaagggc cgtgccttgt tggatgccca
    52201 gcaaaacaaa agccagctgg aagcctgtca aaagggaatc attacgccca ctcctcaact
    52261 aattatgaag atgaccattc aatacattac aaatttagta accaaaaaag gaggtgagaa
    52321 taatggataa catatatccc gaatggaaga aagtcgcttg gaggtttctc cgagtgttta
    52381 tatcagcctt tttattcacc ggatcgatgg tgttgataaa cgcccagcca gtagcactga
    52441 gtagttggga caaatttctc aatactctga tattgccatt tttattggca ggtggggtgg
    52501 ctggcttaaa tgcagtagga aaacttttga gagatttgtt tggcactgag gatcaaacag
    52561 gcctcgtcga caaattacca ttctaagcac tatgaataac aatagccaat ccactgacac
    52621 aaatatgaga gaaagaattg ccaagctcga ggaacgagtg gacggtggat tgggttatat
    52681 caataaagag ctggtggaaa ttaaagagaa ccatctctat cacctacaag ccgatgtaac
    52741 ggatttgaaa atgaaggtta atactctagc ggtgaaagta agtgtgggag tagcaattgc
    52801 gacaattcta atcgatttag tttttcgctt cctactgaag tagtttattg gccttttttc
    52861 tctttcttgg tatctggctc aagtaaattg tggagatcct tagtgaagcc atcaaaggca
    52921 ttggcattta aaacggtgaa ataggtggat ccaggaacac cagtaaagac taaagtatca
    52981 ccaggctgaa tgttctgggc ttttcttagt ttggcaggta taacgagctg accgcgtgaa
    53041 cttaccggcg acgtaccgaa tacaacggtc ttagctgact ttttatcttt tggcttactt
    53101 ttatcgttca tatttgaaat caacttgctt tgagacaggt aaagcaattc cctataatta
    53161 agaatattct ttaattacag tagtcgtatt atacaataaa tcagtgtcga gaagcacgaa
    53221 gactggaaaa tgacagaaag atacagtaac aaccttgtac agacaggggt taagaagtag
    53281 aatatacaca gggtcgacgg tatgtaatga aagaatcgat acccattcgt tctttaagca
    53341 cattaagttt gagttccaaa tcaggagtga ttaaggaacg gaggttcgag ctgagtaccg
    53401 tccccagctc acttatgaga agcgacttat tctttgtgaa tttctaagtg gtagaaaatg
    53461 tgttaattaa agtaatgaaa aatgagtcta gtgtcagttc tggaggagtg gtaataaata
    53521 gtcttggcca gatcctggtt gttaaacaac tgggtgatac gtggtcttta cccaaaggac
    53581 atgtgaacga ggatgaagac attttggtgg ctgctcagag agaggtatct gaagagtcag
    53641 gtgtttctga cttggagttg gttaagggtt tgggttttta ctatagacca agaattgata
    53701 agccggaaga aataaaaaag atctatatgt ttttgtttat aacctattgc agtgagttgt
    53761 tgcctaaaga tgatgacaac aaagaagcag tgtggttgaa tatggctgag gctgtggata
    53821 ggttgtcagc aaaaaaagat aaagagtttt tgatgaaggt ggctagagag aacaatttag
    53881 gttaaaataa gaggaaattg tcgctgtggt gaaattggta tacacgtacg tctcagaagc
    53941 gtatggagca atccgtggag gttcgattcc tctcagcgac acatttagtt aataactacg
    54001 aattaacaat tataaattaa ttggtataat aactttactt ccgccgaagt agccaagatg
    54061 gtcacggcac tggtctgaaa aacctgggat gttggtccga gtccaacctt cggcacattg
    54121 tattgaaata ttaataattt gcgagagtag ctcaattggt tgagcatctc cttgccaagg
    54181 agaaggttgc gggttcgagt cccgtctctc gctcaaaata atcctccttt tgatatgttt
    54241 agggggattt ttgttggtat aatagcctta gcttaaggct gtttagctca gttggtagag
    54301 catcccgttt acatcggggc ggtcgggagt tcgagtctct caacagccac ttggcttgat
    54361 ataataagcc tgtcctttga caatctgccg tcgtggtgaa attggtatac acgtacgctt
    54421 gaggtgcgta tgccgcaagg tgtggaggtt caagtcctct cgacggcacc agtatctact
    54481 gaacagaaat tttattcata ataatgtttt tgtttatctt tgccccgtcg tctaatggta
    54541 ggacagcagc ctttggagct gtttattgtg gttcaaatcc atgcggggca acaaaataga
    54601 acactgaggt tttgatgatg aataataatt ggatatttcc gatagactgt gcagttcgaa
    54661 agtcttgtcc ttatttttga ttagttttag ttgtgttgag ttataattga ataatgactc
    54721 gattttctaa atataggaat agaataatag tggtaataat gtgctttttg gttgctcctt
    54781 ttatttattg gtattttgga cctagagttt gctctattag ccgtcttgga gtgcagttct
    54841 tgtgttctag gggtacagtt atccaatatt ggccaaatat atttgaaaat gaatttgttc
    54901 tagtaaatcc ttccacagct attattgatg agtatcagaa ttctgatgag aacatgtata
    54961 agactggagt ggttatttct cgctatcacc ttttggatga ccaactaagt ataaaaacag
    55021 caagagattt ggttgaatat gaattgcttc agcagacaat ggtggaaaag aatggccaac
    55081 cgccggttgt gcctggttca gggaagttgt tgttgaacgt atctccatct ttgtcccaat
    55141 gggatggtgt ggttgttcag gaaattgttg gtggaggtaa cgtttatatt aatgttaaag
    55201 atggttatgt ttataagctt caagtgaatc aaggaaaatc tttttcagat ttggaactga
    55261 atcaaatatt atcatcattt agatggagca taaaaaatga aagaaaagaa taagatgaat
    55321 aatcatcgtc tagttgctgg aattatcggg tgtattccga ttgtgggtgt ggttggtttt
    55381 tttactcttc ttatgtctgc tttttcgact gatgccccag gacaagataa tagctctgct
    55441 gttgcgattt tatttagtat ttatctatct ttgagtgcag tttatgtttt tgtttttgga
    55501 atatcacctg aaatactttg gtctaaaagt cataagaatt gggcacgatt gttgtgggta
    55561 atatctttac ttgtaaattt gtggttagtg ttatttttta aaaatggttt ttataattga
    55621 tttaatgttt atgaagagac tggaataaca tagactatgt acgtagtgta tatattaaga
    55681 actaatggaa ataccttata taccggacag acaaataatt tggaaaagag actgatacaa
    55741 catcgagaag gtaagggtgc taagtatatg agaaagttta agtattttat acttgtttac
    55801 actgaagagg ttagttctct gtcgttggcg ctgaagaggg agtgggagat aaagaagatg
    55861 gtgaaggtga agaaggagga tctggtaaga caaggggctg tgattcgaga cgatcaagga
    55921 cagtccagtc tatactttga tgggctaaat agccagcgta agaaagaagt gttaaaaaaa
    55981 caaaaataat taggtaccca aaaaaatcta atttttcaat agtcattgtt tattttaaac
    56041 gagaaattgt ataagtggta gggaactcta agaaaacaac cggtgattct tctagtaaga
    56101 acctttggaa atcataatat atattttttc gttcttgttg atcaaatgat aatctaccat
    56161 cttcaagtaa cttatctatt ctggagttgt cgaagtgggt tatgttggta tttttttgag
    56221 ttgaatgcca gaaaatgtat tggtcagggt cgactggtat ctgtccaaat gcaagtatcg
    56281 tttgataatc tagaaggttg gcttgttgat gtcgaagcgt aattttgacg gagatgttga
    56341 atgtttctga ccagcttttt tttatttctt ctgccatccc cattaattgt ctgtcggcaa
    56401 ccattaactc tagttgttcg atttcgtttt tttcaaacaa ctcttttgct cgagtgacgt
    56461 taaatctgta ttcttttact tggggattat atgcccaaga gttgggtgat attggactta
    56521 gacacctggt gtttttgtcg gtagttttag gagtggcgta agcgatggct tgtcgtagct
    56581 gcttattgtc tatcttatct gtatttataa ataaagcaat gtattggtcg gttgggatcg
    56641 aagatgataa cttggtgttt ggccattttg agagttcctc gtttagttgg tcggtagaaa
    56701 tctggtgcac ttcacctaac ttaaaggcat taattaaatc ggtagaattg ttataaaaat
    56761 aataagttaa tcggtcttcc ttggtgtcga tcggtacgag actcagaatt ttaatgtacc
    56821 catctcggta gctaattttg tctactttgt atgacccgag accgacattt gtttttttaa
    56881 aaaatatggg ttcattaagt tttgatagta agggtgaaaa aacttcaggt gcttttattt
    56941 ctaattgatg gggggagatg gctttagagg tagtcccggg gatattgatc tctatatcgt
    57001 ctgcctccag ttctttctga ttgtgccaca atattccttt cttaagagtg aacaaataag
    57061 tcttgttatc atctttcatt tctagactat caacaatggg agagatacta gatctttcgt
    57121 tttcagaggt aatagacagg ccatagctaa ttttgtggct aatgtcttca ggtagtgttt
    57181 gaatggtata aagaccttca atgccaattt tttgtgtcct tggttgaatt attgagtaaa
    57241 tttcggacag attttgtctt tgggtaaata taatcgaccc aaatagaatt ccaccaataa
    57301 tggcaaaggc gtagcttttt agatagtgaa aactgtattc gagaaagaaa cgaattttct
    57361 tgggcaagat ggtagtttta aatagtagtt tgaattattg atgagactag gaacaatatt
    57421 aagataatta tagttatgta aaacataatt ttttcccaac ctcttttttg aaaagagacg
    57481 gaagacataa tgtttcccct ggagttgtcg tcactagctt gtaagaatat taaaacactt
    57541 aagctgacag agagtatgat ttggaaaatg gtgagagctg ttttcatttt ttttcttttt
    57601 taggtttaat aattgattga aaaaagtata gtaggaaatt ggtagaagcg gaactgatta
    57661 catacgtcca gaatccatag aaactaagtt ctggtattga aaacccttgc caactggtgg
    57721 ctggtaagtg gatatttttg acaacaaaat ctgataatag aaaaacagat aaatagaatc
    57781 cgacagtatt aataactatc ctgaaaaaac cgagggttaa gatgttaatc ggaattagga
    57841 ggagtttgat aatgggttta agtaggagtt caaacagggt tagtacaaaa gcaactttaa
    57901 caatagtcca gacaccattt tcaaaagtta aattttgcca aaattggttt tggagagata
    57961 aggcaaatga tattaataag ataaatttaa gaattttttt catgtttttt tgatatttac
    58021 tgttagtatt ataccgctta tgcaaagtaa gagtatggtt gtgccgacaa aaatactcag
    58081 attaagataa tgatttttgt tttccagggt gaactccgtg taaggaacgg aaccttctga
    58141 agtaagatag attttttttg aagacggtag aattgactgt aagaatgatt gcttgggtat
    58201 ttctgtttca gttgaaccga agggtggtag ctggataact ggtgactgcc aactgttatc
    58261 tttgagtttt agggtgaagt tatcgaggct ggtggaattt tggttataaa tatttaaggt
    58321 ctgaccgttt aattttattt taggaggtac aatttcagat gttagatttt ttgtggcaaa
    58381 atctataaaa acggatttgt ttgaattacc atttttgtat gagcctggtg gtggtggcaa
    58441 ttggctgttt aaaccatgga tgacaaaggt gacgtggttt aagtcaagct cattaaagta
    58501 atcaatacca ttggtagttt tttcccaagt tgggtcaatt ggtatccatt gttgagtttt
    58561 gttgtcgtag tattctggcc atgcatgcaa aacgtcagag ttttggttaa taggttttat
    58621 ttttaagtcg ttgctgtagg caaaaccctc gatttctcga gcaggtatac ctttgtttct
    58681 ggctagtgtt ataaaaagat ctgtgaactc ggtacagagt gagctcgatg gattgtctag
    58741 tgcaccaagg gcgccaactc tcttggcata gttaatcctg ttgtagtcgt actctaagtg
    58801 atttatgacg taattgtgaa tgtctttggg tccagacaag ctttggctga tagaagttat
    58861 cttggggtgg ttcacgggcc agtaggtttg gtcaattaaa tattcggcag ggtttatgga
    58921 gtgctgtctt tttatcggta agtgagtttt tacctgacca gtggcggtaa cgtcaatttt
    58981 ttcacctggg cttaggttat aagtggccag ccaattaccg tccccgtcga taatgacgtt
    59041 ttgtggtgct ggctcgatat ttctataagt gacactttgg ttgtctgtat ttggtggtat
    59101 aggaatttgt cgaataatta cctttgaggt gtcgttacct aaatggtagt gtagcttgaa
    59161 atcgaatagt tgatgatcac caaaaattaa aagtattttt ttttcaagtt gagtttggtc
    59221 aaattttatt tgagtagact ggttaagttg gtgtgtgcta ttgactggtg ttgaagaaaa
    59281 agacagattg ccgaaataag aaggtacctc aactgttact tggatgatac tgtcgttaaa
    59341 tgaatgtttg taaactggga gctggacttc ccaagtatta ccctttttct tggcaaattc
    59401 tgaaatggta tatgtaagga aaaactggtt tgtctggtct ttacctatta tgggtttgtc
    59461 aaacttaact aaaattttac ttaggttttc tgcagaagaa gtaagttgtc cgccagcgat
    59521 attgtctatc tttgatccgg atatgctgaa ttcatattct ttggcgtata tttgcgaaat
    59581 gttgtttgtt agattgattt cttgtttaac gctggcattt ccactttggt caacaagata
    59641 atgaactttt tgattaactc taaattcttc gagtgctctt gcgggagaga taaagagaag
    59701 gaataaagtt aagaaaaaga aaataaacat gatagtacta ctatatcaaa tagacaaata
    59761 tcttgacttc gggtattgtt cgtgtcatgc tactaacatg acagcaactt tatataaacg
    59821 acaaaagcag atacttgaat atattaataa taatattgat aaatttggat gtgctccgac
    59881 attaactgag attgcttctc atttgggatt gtcgtctctg gcaactgtgc atgaacactt
    59941 gggggtattg gagaaaaaag gtttgataag acgttatcga ggtgcagtga ggggtatcga
    60001 agtgttgggg cagaaaataa aaagtaaggt gtctcaactt gtagagttgc cggtattagg
    60061 ttttattgct tgcggagctc ctttggagcc atatactgat ccaaatgcaa ctttggcggt
    60121 taattctagt tttgttaaag agggtgatga ttcttatgtt ctacaagcaa agggtgattc
    60181 gatgatggac gacggtattt tgagcggtga ctatctagtt attaaaaaac aaaaaagtgc
    60241 taataatgga gatatcgtgg tagcagtgtt gtcaaatggg tttgctactt taaagagatt
    60301 ttatcgagaa gcagatagag tcaggttgca gccagccaat tcaacgatgt cccctattta
    60361 tgttaaaaac gtagagatac agggtaaggt tgtggcggtt attagacaat ttaattaaaa
    60421 actagaatgt tgtatataat tgcgacacct attggaaatt tgaaagatat tactttgagg
    60481 gcagtggaaa cattggagca gattgatgtg ttgttgtgtg aggacactag aaagactggt
    60541 ttgttgttgc agaatttgaa gattggtaac aagccaaagt tattgtcttt ttatgatgag
    60601 gttgaacaac aaaaaatacc tcagattata gaaatgttaa aagaagggtt ggaaattggg
    60661 ctggtgacag atgcaggtac ccctttgatt tcagatcctg gatggcttct agtcaaaaag
    60721 tgccaagaac taggtttaag ttacacggct ttgcctggag cttgtgctgt ggttaatgct
    60781 gctgttttgt ctgggttacc aatacagagg ttctgttttc ttggctttat gccaaagaaa
    60841 gaaggtgata ttagaaaagt cttggaaaaa tacaaaggtt ttgatggtgc aaaaataatt
    60901 tacgaatccc ctcttcgaac tgagaaaact gtcaaaatag tacaagaaat gggtggagaa
    60961 gttcgaataa ttcgagaaat gacaaaaaaa tttgaagagg taaagacgaa tcttgatggt
    61021 gatactagag gtgaggtggt gattgttttt acttagatac cttccttttt tctgcccagg
    61081 agacaacttc ccgagttcga tcgatagcat cgggactaac agatatactg gtgactcccc
    61141 aacgaacaag tttttcgaca aggtcaggat gattgctggg tgcttgtccg caaattgaag
    61201 aagttattcc acgctttttt gccttagtaa cggctctttt aagactccaa agtactgctg
    61261 gatccatttc gttgaaatct ttagctactt caccattgtc acgatctagg ccaagcgtga
    61321 gcatggttaa gtcgttgctg ccgatactga taccatcaat tccgacatca atgaatttat
    61381 cgagcaggat aacgttgctg ggaatctcga ccatcataaa taatttgaag gtaggtgatc
    61441 ggagtagccc atggtcagtg atgatttgtt tcacccgggc catttcttgc ggtgttctga
    61501 caaaaggaat cattaaccag aggtttttta aattaaattt gttacgaacc attttgatag
    61561 cctcaagttc catactaaaa acatctggag agttgatata acgtgtagca ccgcgaaaac
    61621 ctagaagtgg atttggttct tcaggctcat agagtttgcc ccatttcaga aagcgatact
    61681 cgtttgtttt gaagtcagtg gcccggtaaa ttactggacg ggggttgaag tttttacaaa
    61741 actgagatag tccgagtgct agtttttcta tatattcttt ttgtttaccg tcagcaatca
    61801 ttttttttgg gtggatgccg atattagcaa tcataaattc ggctcggagg agaccgacac
    61861 cgtcgacatg acgcttactg acggtctttg ctagttctgg ctcgccaagg ttgacatata
    61921 ttttagtagc agtcttggtt ggagttttag agccgtggtg ttttggaacg ttagtggaag
    61981 ggaggcttga cataacgttt ccgtgccaga tttctccggt agtaccattt atggtgacga
    62041 cttctccttc tttaaaattc ttggtggcag tcttagcccc aacaacacaa ggaacaccta
    62101 attcgcgact aacgatagca gcatgggagg tctgtccgcc tttatcggtg attatgccat
    62161 taacacgttt catagcaggg acaaagtctg gtgatgtcat tgaagtgaca aggatgtgtc
    62221 cttttttaac tttgttaatt tctttggggg atttaatgag aacagctata cctgtggctg
    62281 tacctggact ggcgggctct cctttaaggt ctgcctcttt gctgatgtct acttgtttgt
    62341 caattgtttt ttgattgctg gtgatggtgg tgattggtcg ggtctggaca atataaagtt
    62401 gatctttgta aatagcaaat tctatgtctt gtggtttgcc gtaatgattg tggagttttt
    62461 gacctatttt ggcaattttt ttggccattt cgtcactaat cttagctttt ttttgcttgg
    62521 atttagggac tgaaacctct cttgtttctt ggtttgattt tactaattga acatcttgag
    62581 ttacgtgatt tttagagatg atgctccaat tgcttttatc gagaattatc tggtcagggg
    62641 tcacttggcc ttggacaata tattccccga gcccccaaat tgtctcgaca attatttcat
    62701 ttttgttgtt gtttactgga ttgactgtaa acattattcc ggatacttcg ctttgaattt
    62761 gtttttgaac tgggacggct acgccaactt taaaatggtt gtatcttttc ttaactcgat
    62821 aaaatattga acgaggggta aataggctgg cccaacaatc tctaactctt tgaacaacgt
    62881 ttgcttcacc gatgacgtta agaaaggttt cttgttgtcc agcaaaggag gcatcggcgg
    62941 aatcttcggc agtagcagat gaacggacag ctactggcat gtcttgtaaa ctggcaaaac
    63001 cagagagttt tttatagcct ttcataatat cgatggcaat atcgttgggc attgggctag
    63061 ttttaataag ttgttttatt ttgcgtgaag cggcaagtag ttgatctggc tgatcaacgt
    63121 cggtggtttc gagtatgtca ctgatttttg gttttagatt gttttctttg acgaaacgaa
    63181 aatacgagtt agaagtaacc acaaagccgt tgggtaccgg aatacccaga ttgtacattt
    63241 ctcctagatt tgctcctttc cccccgacga gaggaacatc ttcaaaacta atttctttga
    63301 accataagat gtttgtcatt gttggtaacc tcgattgggt gaatttctaa ttgattcggt
    63361 ttctgtgggg cgggagtagt cgtcttcgat tctgatagtg ttacccactt ctggagttga
    63421 ggcttcgata gtgacacagt cagtgatggc ttcaaaacga tgaatggtat tggtggtaat
    63481 tgtatagcca gtgttgggga tcatgtcaaa aattgaatct cctattctga acttggcttg
    63541 accagagagg agggtgatgg tttcagtttt ttggtcgtgg ctttggagag accagcgttg
    63601 acctgctttg gtaaaagcaa tcttactggt atatggcaga ttatcttcgg ttagttttat
    63661 ttcaaagccc cagggcttat ttatttttat agacactata aaaataatag cagatttact
    63721 actttactag tggggttagt acacgaatta aggctgtttc tcggggtaag gacagttgtc
    63781 ctgttttgta ttggaactcc aattcaatta cttggaggta agcctttttg agtagattct
    63841 gatcaaattt ggaactagtt tggatttgtt tgcgaaaact acctttaagt aaataaacaa
    63901 acagatcaaa taaacctgaa gagacaactt gttcaaatag tggggcaaaa ctatttaact
    63961 gatgaggcct aatgctgttg aggcaagcaa ataatttgtt atcaagtttg aaggaaagta
    64021 tttttggttg agaaaatact ttttgttggg taggtgtaat tgttttgtcc tgccagatga
    64081 gtagatcggt tgaattacta tttataatag tgactaattt gtctagaatt ggtttggaga
    64141 tagagaagag gttatcgata gctaaagttt ttgaatcctc aaataaagag ccgctactga
    64201 gaaagttgtt gacttggttg aggtcgatgc ctttggcgtt aatctggagg acagagccgt
    64261 tctttgcttg agagatagcc tctagataag cgctacgtga ttgagcagtg ttgtcaccgt
    64321 gaaatattgt tatcacaagg tgactccgga gggagtcgaa ccctcgattg cctggatgaa
    64381 agccaggagt cctaggccac tagacgacgg agccgtttaa taatggagta ttttaacatc
    64441 tgtaaagtgc taattatagc ctttagcctg aagaagaaaa gatttggcgt aaagaccatt
    64501 agctttaatt aaatcatcat ggctcccctg ctcgacaatg ctgcctttat cgatcacgat
    64561 aattctttgg gcgtttctga cggtagagaa gcggtgagac acgataatta aggtgttacc
    64621 atggatgtct tggtaaagtt gattaaaaat tttgctttca gaaacagcat cgatactggc
    64681 tgtgggttcg tcaagaataa ttatagggct gtttcgataa aaaatccttg cgatagcaat
    64741 tttttgcatt tgaccccatg aaggttcttc accgtcgtat ctctggctaa gcatggtatc
    64801 tagaccttta gaatatttgt ctacaaaact agcatcggct tttttcaggg ctgataacac
    64861 ttgtttatcg ttgggtttgc ctagtttacc gatagtgaca ttgtcgcgta agcttaaatt
    64921 gccatagcgg ttgaattctt gggatagata tgacaactgt gtgtgccaag attctatgtt
    64981 gatatctttt aagttgatgt catttataaa tatattaccg gaggttgggt cataaaaacg
    65041 acagattaac tttactaaag tacttttgcc ggcgccattt tcaccgacaa tggcgatttc
    65101 ttcactggtg ttgatagtta aatttaggtt tttgtaaacg taacgtttgc tgttgggata
    65161 tttaaagctg acattgtcaa aacggatagt gggagggaca gagatgttga tttcttggga
    65221 tccactaaca acgacatttt ttaagttcat aacttggcga aaattgccga tgtggtagct
    65281 attttcggtg atgctaaccg agttgtagac tagactgcta aaaaaatcgc cagcttgggt
    65341 gattgtttga aaatagaaag taaacagacc aatagtaaga agaccatcat gggtctgtcc
    65401 aacaagcgta taaaggcaat aggctgtgat ggaaatagaa agagcgatta gcccgaactg
    65461 agttggggtt tgacggtgaa taagattaac tcggctgttg gtaaagttat cgaaagttga
    65521 ggcaaatttt tgaaataagt attttattgt gttgttgata cgtaattcag caatgtagtt
    65581 tttggtgcga agttgataat ttaggtccca gatgagcttg ttgttctcag cgttatcttc
    65641 gtgtagttgc cagtttttct taatccagta gcgttcaata aaatactggg gtatctgacc
    65701 gataaatatg gccaaccata atagaggaaa ttttggaagt agaatgatgg tgacggtggt
    65761 gaggctaatg aagttggtaa aaatattgtt aatgtcacta gcggtggctc gaatgcgcca
    65821 accaaattct tcgtcaactt tggagattag attgccagta tctttgcctt cgaggtgttg
    65881 gtaatcaagt tcggaaactt ttttgataaa tatcttgcgt aagtagtttt ggagacggat
    65941 atcctggatg cgactaaggg tggagttgag atagaaaatt gaagaagaga aaatcaaaat
    66001 cccaatgaaa gagagaaata tattaattag tgatgagttt tggttagatg aatagacgat
    66061 gttgtcgatt agttgggaga aagtgtggtt acgtaaaaaa ggggtggtgg caacgagtag
    66121 ctgtaaaaca ataacagaga tgtagagaat ccggttggaa ttgaagacta attgaataca
    66181 ccaaagtatg tgactaagag tgtcggtttt tggtttggcc atattttagg agaagtatac
    66241 caacaataat taagattatg ctaataatct gtccggtttt aacggtagaa attacccaag
    66301 tgtcttcacg ccagaattca gtaagaaggc gaatgagacc gtatccgata aaatatttag
    66361 cagttgggtc gtaggggtat ttttttataa gtaagaataa gagtaagtta ccaattgctt
    66421 cgggccacca aataggattt tcaccattga tgtaattacc aaaacggccg atggcttgag
    66481 atagaggcag aatgggggta atggagtcaa ggagcatgag atatgatttg tgatttacga
    66541 atagggcgat aaagacaaag gctccaagga aagctccaaa gatgccgagt cccccattcc
    66601 atgtttgggg aattaaatat aggttttgag aatagaagga ccattggtcg ataacatggt
    66661 aagctctagc tccgattatg gctgagacga tggttcctat ttcaaaaaag gcgagttttt
    66721 ccttaggaat tatactttga tgtttttcaa aatagctgat accgatgatg atggcgatgc
    66781 cgataataag gccgtaaaga tgcatgtgtg attattatac gttttattgt gagcaattgg
    66841 ttggattttt attttagacg tgagataata caaagaattt cagggtcctt agctcagtgg
    66901 tagagcagtt gccttttaag caattggtcg cgagtccgat cctcgcaggg cccacccaaa
    66961 aatatctggc gtgtttttta agtagaatat tcccaataag aacggtgaaa ctgaactagg
    67021 cggtttttct ggtacgtgcg tcacgtaggt agtagagttt gcttcgacga attttggtct
    67081 ttggtgaacg catttttttg atgctatcaa tccatggaga agctagagga aaaattcttt
    67141 ctaccttgac actgtcagaa gcagctttct gaacgataaa acttttgaca tcacctttgc
    67201 cacgaatggc gagaagtacg ccgtcgaaag cttggacgcg ttctttgtct ttttctttaa
    67261 ttttgtagtt aactttgacg gaatcaccta cagtgaactc ttgatcttgg aaaatgaatt
    67321 ttaatgacat agtagggtat tttatattaa tgtagagagg ttatcaataa tttgcttttt
    67381 tatgataaca gagttatgat gtagtgtaac agtatgaatt acatagttgt tgcatatctt
    67441 aaccgccgat gggcggcttt tttttgccaa aaatttataa attcagtaaa ggaggtgaa
//
LOCUS       DDWP01000021            4504 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_22222, whole genome shotgun sequence.
ACCESSION   DDWP01000021 DDWP01000000
VERSION     DDWP01000021.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_22222"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1581 a          697 c         1070 g         1156 t
ORIGIN      
        1 cgaacaattt tttctaagat aaaaaaaata attattccag aaattgttaa aaaaggtaca
       61 actagacctt caccaattgt ctcgctagct tcgggtaaaa gatggataaa agcatcaccg
      121 aaaagagtac cgacagccaa actaaccaaa taaacaatat aacgagaaag attggccacg
      181 cgaccaagca aataataaat gataataatg gagatgaaac taacggaaac aacagaaaga
      241 agggaatata tcagagttaa catgtggata gtttaacatg gggcaaattt tgatatgatt
      301 gattgatggc attagaagat attaaatcac cgttaccacc gagaaggttt tcgggcctat
      361 acgaaacaga gacggaagag gaaaataaaa atcaaaactt aaacgtcaaa actcaaaacg
      421 aagaagtaga ggtggaaccg gtggtgccat taaatgcacc actgattagc ggtaagatag
      481 aaaaaaaagt agatcacaaa agttatcttg tatatgctgg gattggggtg gctgtagtgg
      541 caatactagg actggtattt ggagtgataa ttccaaaagt gatgggtggt tcgaaggtgg
      601 aagaagtaaa cctaacctat tggggattat gggaagatag ctcaataatg ggaggcataa
      661 tcgcagaata cgaagtggct aacccgggag taaaaatcaa ctatatcacc aaccagaaag
      721 taaattaccg aacgagatta caaagccgac tggaacaggg tggtgatact tcagccccag
      781 atatatttag acttcatagc agctggatac cgatgatgca agataacttg gctaaagtac
      841 cgaatgatgt ggcaacagga attgggctag ataccgatta ttttgatgta tataaacgag
      901 gtattaaaga tggcgccaac tacttaggca taccattaat gtatgacgga ttggcccttt
      961 tctataataa agatttgatt gaatctggtg gagtaacttt gcctaaatcg tggtgggacc
     1021 ttgaagtggc ggctaataag ctaacagtta aagacagtgc tggccagatc accgtggctg
     1081 gagtagctct ggggttgacc gataatgtag atcactggag tgatgtggtt gggctaatgc
     1141 tcaaacaagg tggagtaaag atgcttgacc caggagaaga tagtacaaag aaaatgaagg
     1201 atgtgctgac ttactacacg ctatttcgaa ctaaacatga agtgtgggac gagacattac
     1261 cgaattcaac tgaaatgttt gctaacggca aactggcctt ctattttgca cccagctgga
     1321 gggcttttaa tattgaggat atgaaagtgc cggagttgag atatggaatt gccacagtac
     1381 cgcagttacc gacattaaat gatgtgcccg tagatcaaat taataacgaa gctaatttaa
     1441 ctaatattca ctggtcaact tattggcttg agggtgtgaa taaaaaaagt agtaaacaaa
     1501 aagaggcatg gaagtttttg acttatcttt ccaagaaaga gagcttggag aaggtgtttg
     1561 caactgcttc tcaagtgaga agctttggag aaatatatcc aagaaaaagc atgtctgcta
     1621 agatgtcgga gaatccaaac ctggctccat ttgtaaaagt ggctaatgaa gcagatggtt
     1681 ggtacttgtc gtcaaggact tttgacgaag gattgaatga tgagatgatt aaatattttg
     1741 ctgatgcaat taatggaatg gtactacaaa gcaggacacc agaagaagta atgccaaact
     1801 tgagaaatgg aattaatcag ttgatgcaaa agtatcaact aaaataaaaa cctaaactct
     1861 aaatcctaaa acaatgattc taataaactt caaaatatac aaagagacat ttggagataa
     1921 ggtggtggaa ctggcaaaag ttataaaaga agtaggtgac aagtataaaa taagaatggt
     1981 ggtgaccgcg tcggctctgg atgcgttaag gctaaaagat ttaggagtgg aagtgtggtt
     2041 acagaatgta gaccaatatc ttgaaggtaa acatacaggc tgggtatcag ccgaacaagc
     2101 aatgtcttta ggaattaaag gcgggttggt aaatcacttt gaacatcaag tagccagagg
     2161 aacagcacta aaaataatta aaaacaaacc gaaagggttt gagataatgt gttgtgccaa
     2221 atcaaccgga cagatagaaa gatggatggc aagcgccaaa cctgattaca tactttatga
     2281 accaccagaa ctaataggaa gctcgaccga ctcggtggcg agtaagccag aaagtattaa
     2341 gcgagcagtt gaattgtgtg gtgatgtgcc actaatggta ggggctggag taaaaagtaa
     2401 agaagatgtg ctggtgtcgc ttaaaatggg agccaagtca gtaggattag caagtggatt
     2461 tgtattgagt aaggacccaa aaagggtgct ggaggatatt gcctctggat ttgaaaccca
     2521 ccctaccctc ccttaaaaag ggaggaatca cacaaaaata ctttatcttg ttataattac
     2581 aaagaaatat ggcatcgatt gataatctat tagtttcact gttcgttgac gaagttgtcg
     2641 gcggttttat agtaactgtg cccccgggtc acgtaggatg tatttacagt atctttcaag
     2701 gagtacttaa gaatgtgtgg tacccaggga tgcattttaa gattccaata attcatagag
     2761 taaaactttt taacgctcaa ctgatcgagt acacggtatc taaggacgtt gtagttaaag
     2821 acaataaaga aatcatgggt gatgaagtta ttaactcagt gacagctgat aataaattcg
     2881 tagctgtaga agcaactgcg ttaataaaac ttgataccga aagattgcca gaaatatggc
     2941 aaaatatcgg agaaaatttt gtgtctaaag tggtcagacc ggtaactaga agccgaatta
     3001 gaaaagtatt aactaatcac accttagcta aagcactatc gactaatagg agtgcgatcg
     3061 aagatgagat aaaacaagag ttgcgtaatt ccttgagttc acatggactg gaagttgaga
     3121 atttttatct tagttcatta accccaatag aaggtagatt gattgaagac gcgacagaag
     3181 aaaaaatagt gatgccgaca gttgtggccg atggacaaaa aagtgaagag gaaataagac
     3241 agagtagagt aatcagtggg aatatgccta ggcaatagtc aatatccata tatccgaatt
     3301 ttcaggtaag caagatatag cagacctcat agtggaacca gtggtgtaaa cgtcgtcaac
     3361 caggatataa ccccccttag tccccccttc aaaagggggg ttgagagcaa aggctgaatt
     3421 aatattagtg tggcgggaga gcttgtcttt gatacttgtt tgaggagtgg tatctttggt
     3481 tctgattaat aaattttcgt attttagatg tatgagagga gatagctctt tgaccaacaa
     3541 ttcggtctga ttaaagccac gccaattgaa acgatgttga tgaaggggta tggaaactaa
     3601 gacaaattgt tcttcttgcc aatattttaa taaagacggg aactttgatt ttaaagtacg
     3661 gctcatgagt agagccagct cagggataat gtcggagaca aattcaaatt taagatcgtg
     3721 aaccatagct ttgataggat catggtagcg aaaaagagaa agacgatcga aacccacctg
     3781 atttaaaacc ccctgcccct tcgcaagctc ggggctgccc cccttaaaga atgggggctg
     3841 ggaatgttta atcgagcgaa cgactaatct agattggcaa tcaagacaga gataacgact
     3901 gttcttacca cagccataac agtgacgagg aaagatgaga tttaaaatcg acacaatata
     3961 ttcaatcccc cttgatcccc ctttgacaag ggggaaatag taaaataaat tatgaatttg
     4021 gttaagaggg aaggagcaag tgggttagtg atgacagtat tgatttggtc ggtggtaagc
     4081 gtgttgggaa caagattatt tctaagcttg accggaaatc caacaatcgg gtatggccgg
     4141 tggcatattg cccacgtact gtttggaggg atatttatga tgctagcgat gatgttggaa
     4201 ttaattttta aaaataaaga tttaaaaaaa gtagtggcaa ttatgtttgg aattggttgg
     4261 ggattattca tcgatgaggt cggaaagttt ctaacacatg ataacgatta ttggtttagg
     4321 ccggcagcaa tgatcattta tgtgtcattc ataatgcttt ttctaaccta taaatacctg
     4381 gaaaggaaag ataaattagt taagaagagt ggtaaattcg ctagagcaat agacacaaca
     4441 ataactagaa tggttaagaa aaagatagtc gcctatggct tgtggctgta ttcaatatat
     4501 tatt
//
LOCUS       DDWP01000022            3439 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_44611, whole genome shotgun sequence.
ACCESSION   DDWP01000022 DDWP01000000
VERSION     DDWP01000022.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_44611"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          934 a          607 c          807 g         1091 t
ORIGIN      
        1 ggggggagga gggaaaaaaa taaaaaacaa aacacaaaaa ttaaaactta aaaaaagatg
       61 tttggagttt tgagttttaa gtttggtgtt tattaatgta cctaattcgc ctgtttggcc
      121 caggccccta tattggtcgg tctaggcata aatacccact ttcgactccc ctagtataaa
      181 aaatacctaa aaacaggcat aatatgtata tggctttatc agaggctcta gaaacgacga
      241 aatttgtctt cgggtgataa tattaatcat ggatttaaat ttaacgacct tggtgcgacg
      301 ctttcttgaa tatttggagg tggagaggaa ctgttctcgt ttgacgatta gaaactacca
      361 gcattacctt gatgtgttag taacttactt ggtggaatct gggatatctg agccacgaat
      421 taaagacgta aatcttgaag aaataagaaa gtttaggttg tttttagcga ggcaggagct
      481 taagtctgtg actcaggggt attatgttat tgctataaga tcgatgctga aatggtgcgt
      541 taagaatgat attgaatgtc tgtcaccaga gaagttggag gtgcctaaga cagcagaaca
      601 ttccttgaag tttttggatg atgatcagat atcaagatta cttaatcagc ctattttatc
      661 gactaaagtt ggcttacgtg ataaagcaat tttggagctt ttgttctcga ctggacttcg
      721 ggtgtctgaa ctagtagggt tagatcgaga tcaaattaac ctaaagacta gagagtttgg
      781 tgtggtgggt aagggtggaa aagctagagt tgtcttcgtg tctaaacatt cggctgagta
      841 ccttgagcgt tatttaaggt caaggacaga taagctcaaa cctttgttta ttagaatcgg
      901 cgggaaaaaa gaaatatcat cgaccgatga ggagatgcgc ctaactgtta gatcgataca
      961 aagactagta aaacattacg taaccttagc caaaatacca gtgatggcta cgccgcatac
     1021 actgagacat tcaatggcca ctgacctgct taaagctgga gccgatatta gatctgttca
     1081 ggaaatattg ggacataaaa atatatcgac aacacagata tatacccatg tgactgatgc
     1141 tagactgagg gaagttcacg ataaatttca taaaggtaat cgctgattta gcaaaaaatt
     1201 ataagactta gtcccctctt ttttattgat atattttgga tgtcctatag ttaggtgcct
     1261 attctaggag tgaggtagcc ttacacattg gttactcccc taccctatct tttagtgttt
     1321 tagccgtata atagggttaa aatagaattc tgaacctgac tagcagtggt gtcacttgac
     1381 tgctaaaagt ggtagtccta taacgtgagt cctataacac tgaataaaca ggtttgggtc
     1441 agtagtccta taacaatgag tctttttgtt tatgccgtat tgttaggcat attgtcttcc
     1501 ggtactttgt ccattactgt catggcattt accttgtcat ctgcggcctt gagcctcata
     1561 aggatgacac ctttggtgtt gcgatttaga acaggaatgt ttttgatagg cagattgatg
     1621 gtgatggctt tcttggagac gaggatcacc tgctcggctt tctcggagac tacttgagct
     1681 acagcaatag aaccagtctt actagttagg ttggcgactt tgactccgat gcctcctctt
     1741 ttttgaagtg ggtagagata gacattactt ctcttgccaa ctcctttctc agtgactaca
     1801 agcaaatgtc ggaagaattc accatttttc tctttgttgg tagccgatag agtctgtggg
     1861 acgatatcca tactaactaa ttggtcatct tctttgagag taataccttt gacacctcga
     1921 gtatgtctac ccataggtcg ggtatctgtc tcgttaaagc gaatgcattt accatcatgg
     1981 gtaactaaga aaacttgatc attaccacta gtatgtctgg cccaagcaag ctcgtcttgg
     2041 gggtcgagct tgatagctgt cagaccagtt tgtcgtatat tttggaactt gtcgatagaa
     2101 gttttcttga tagtgccttt gttagtagct aaaaggatgt atttgacggg caattcttta
     2161 cttagtggta aaatcgtttg gatagtttca ccttgaataa tgttgattaa gttaattact
     2221 gcctgacctt ttgattgtct ggaactttcg ggaacatccc aaaccttggt tttgaaaacg
     2281 cggcctttgt tggtaaagaa caaaaggtcg tcgtgggtgt tgacgaaaag catgttctcg
     2341 acttcatctt cggtcttggt ggacatgccg gaaactccga ccccgcctcg cttttgcata
     2401 cggtaggttt ctttagcgac gcgtttaata tagccagttt tggtaatagt gactagagta
     2461 ggttcattcg gaataagatc ttcttcggaa aaatcttcga gacctttgac gtatattttg
     2521 gtacgtcgag tatcggtgta tttttccatg atttcgttgt tctcaccttg gagaacagca
     2581 atcattgatt ttggtttggt gacgatgtcg gttaattgat cgatgacttg tttaacggca
     2641 ttgtattcat cttcgatttt ttgacgttcg agggcagcca gacgacggag ttgcatttcc
     2701 aaaatagcat tggcttggag gtcggtgaaa ccaaacttag acattaagtt ttctcgagct
     2761 tcatcggtag ttttggaggc acggataact ttgatgattt catctatggt gtcaagagca
     2821 atttttagtc cttcgagaat gtgggcacgc atatagtttg acttgaggtc aaaaatggtg
     2881 cgacgtcgga tgacactttc tcggtgacgt aaaaagagta ataacatttg gcgaaggtta
     2941 agtgtctgag ggacaccatt aactagggcc aacatgttgg cagaatatga tgattgaagg
     3001 gcggtgtatt tgtagagttt gttgagtatt gattttggtt tgccggtttt cctgatttcg
     3061 acgacaactc ggataccatg acggtcggat tcgtcgcgaa gatcggagat accgatgatt
     3121 ttcttgtcac gaactagatt ggcgatcttg ataaccaagt cggctttgtt gacttggtaa
     3181 ggaagctcgg tgatgataat tcggatcttg cctgacttgg tctcttcaat gctggtcttc
     3241 ccacggatgg aaaacttacc ccgtccagta ttatacattt caataatgcc tgttttacca
     3301 aatatttctc cggcggtagg aaagtcagga ccttggacaa atttgactag ctcttcgacg
     3361 gtagcggtgg tctcaaaatt gaaggacata ttttctagag cgtagactgg ctccagaact
     3421 ttttcttggg tggtctccg
//
LOCUS       DDWP01000023           10993 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_17917, whole genome shotgun sequence.
ACCESSION   DDWP01000023 DDWP01000000
VERSION     DDWP01000023.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10993)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_17917"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    5816..5839
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3420 a         1402 c         2826 g         3321 t
ORIGIN      
        1 tttgtctttt attcgggttt tggaaatgat atgatggttt atgatctcaa gcttgaaggg
       61 aaaagttgat agaatttttg gaagtcatat cgaagtggag gttaatggag ttggttattt
      121 ggtgtttgtg ggtaaaaaaa gttttgcaga agggcaagat gtaaagatat ttatttatat
      181 ggcagtgtcg gagaatgata ttagtcttta tggttttgaa aaatatgaag atttggattt
      241 atttaaaatg ttaattctgg tgtcaggggt agggccgaag acagccgcag ggatactggc
      301 cgaaaagggg tcgatagaga ttttaaaagc gattggtgaa gccgatgttg atttttttaa
      361 gaaaattaaa gggatagggc tcaagactgc tcagagaatt attgtagatt taaagtctaa
      421 ggtcgggggc ttgggagaat tggatttgag tggtaaagtt gatgaaggtg atgatttgac
      481 tttgagtttg caacaattag gttttgaaag aaaagaaatt gaaatggtct tgaagaaatt
      541 accaagtgaa tttgtggaat tggaagaaag attagaatgg tgtttacaaa atttaggaag
      601 atgagatgac aactgagccg ttagacccaa agctaaaaac agaagataaa gaagttgagt
      661 taagcttaag gccaaagaaa ttgtcggagt ttattggtca gtcaaaattg aaaggtcaat
      721 tagatgtatt tattcgagca gctttggctc gaggtgaatc gcttgatcat attttatttt
      781 atggtccacc tgggctgggg aagacaacat tggcaatgtt actttcagga gaaatggggt
      841 cgaatattaa gattacttct ggtccggctt tgactagagc aggggatttg gcgtcgattc
      901 tgacaagtct taaaaaaggt gattttttat ttatcgatga aattcatcga cttaacaaaa
      961 ttgttgagga gacgctttat agtgcgatgg aagattatgc tttggatata gttttgggaa
     1021 aaggtccatc agcgcgatcg gtgagattaa aattacaaaa atttactata gtgggggcga
     1081 cgacgcgaat aggtttgatc agtagcccga tgagagatcg ttttggattg gtacaacaaa
     1141 ttgattttta tgatgatacg aatttgtgtc agatagtaga gagaacggca aaagtattaa
     1201 acactgagat taaaaaagat gcggcggagg agatagctaa gagagcacgg gggactccgc
     1261 gtattgctaa tagattgtta agaagagtca gagattatgc acaagttaat tctgatggag
     1321 tgataaattt aaaagaagcg aaggtggcgt tgataaattt gggggtcgat gaaatgggtt
     1381 tgtctgatgc agataggagg tatttggatg tggtcaaaaa tcagtatggt ggtgggccgg
     1441 tgggggtgga gaatattgcc gcggcgctga cagaagatgt ggggacgatt atggatgtgt
     1501 atgaaccgta tttgatgaaa cgtggactta taaaacgcac acccagggga agagtaacaa
     1561 tagaaaaatt ttaatgttta agtttcaaat ctgaaaaatt ttaagactat agcgactaag
     1621 ctagctacgt aaaagacgta aacaaaccat gcgggatatt tttgagaatc agtatcaaca
     1681 ccttgtcgac cttgtttttg acgaatcaca tgattgactg aggccataat taaggcagga
     1741 aaatggggga tgaaagccat aacaataaat ataatattgt cgatgaaaat tgtatggccg
     1801 tcagtaagag gtaacaaggc aataaagaga taccgtaaga ctagcgttag tgggtaattt
     1861 aataccttaa gaaaatagta gtagcgatcg gcaaaggtgt attggatatt tagtttcatt
     1921 atcaatgatt atataaccaa aaaaggagtc cttcgacaag ctcaggaccc cttgaaaggc
     1981 gatgtcaatg gtagagacgc catgcatggc gtctctacaa agaaaattta ttctgtttgg
     2041 attccgagca accacaatac agcttctttg cggtagcggc gatcaccacg agcgttgatg
     2101 cggatagctg gtaacttacc acgcttaccc cagcgcttaa ttgttaattt agaaacacgg
     2161 agtaggtcgg cgacttcggc gacggttaga aggtcgggaa gattttcgat gctaacaccg
     2221 tttacctttt taggggcggt tggttgtttc acaagtttat cttgtgatac tgtttgttcg
     2281 tccattgagt ggctcctctt gtatcaatgt aatgtttaac tataaccatt aaaacataga
     2341 gcatactagt gggtcaagat aacaaaatta tgaattacaa attacgaatt acaaatgatg
     2401 gatagtgggg gagaaagatg ttttgggatt gggcgttgag ataattggag tgccaatatt
     2461 tttttgactg agatacaatg ctatatggct ggagacatgg aagttaagca tgttgccgaa
     2521 gagacaacta cctctattga taaggtggcg ggagtaattg agggttctcg ttatgtaggc
     2581 gataaaaaag atccagtgat atttttggaa aattgtggtg taaatcagga ggaatggtcg
     2641 gatttttatg gtgttgttga tgcggagaag ctaaaagttt tgagttattt ccctgccggt
     2701 gaggtattat ttggaaacgt tggcattatt gttgctggta ctgatcaaga aggtgtagga
     2761 atttcatgtg cgtctacagg atttaatcga aggtctgagg attcactggt gatagatggt
     2821 gttgtactga ttaatatgaa gaatgttgac cttatccagg aattggaagg agaaggtaga
     2881 attgaaatta ctcaaagaaa gattcgtcat gaattggctc acctagcctt gtcaaattat
     2941 gtggcaagac aaggtttgga tatacatgat gatgatctta aaatattctc tgagttggtt
     3001 gcagtttgga tcgaagaaga tggcgatatt gataggatga aatcgttggt ggatactctt
     3061 gatcctgaag aacttatgga aatttcaaac atatttccgt ctgttttaga ggatggggat
     3121 atgtacagat ttttacctgc attttttgcc cacatttcgg aaataacttc gggtggatgt
     3181 acacctttaa ggttgctgga taacatggta aagagtaggc caccaagaga acttgatctg
     3241 attgatgaac aattagatgc tcctgaaaag atgcttgtat actggttgtc tgaggcagaa
     3301 aatatagcac ggtcacctga atctaatgga acatatggtg gtacaagaga aattccggtg
     3361 gtgacaaaag gactgttaga caaatacttt aaaggtaatt ataatctaca ccttgatgag
     3421 tcgtggagct cgtggcgtgc ggggctgcaa atatagtaac agtaatttga gtgatagaat
     3481 tatttatgaa gattgcgttt tttggagtga agagttggga gagagaattg attgaaagag
     3541 aaattattaa tttggatgca gtaggggtgg ggatattttc gacagaggtt caagatagtt
     3601 tggatttggc ttcagagtat gaggttatct cagtgagggt aagagcggat atgtgtaaag
     3661 aggttttgag taaattgcca aagttaaaaa tggtagctgt aaggtctact ggtatagatt
     3721 gtgttgatga agtggaatgt aaaaagaggg ggattgtaat taagaacgta ccttcttatg
     3781 gagagaactc ggtggcagag tatgccatgg ctttgttgtt aacggtggcg aaaagaatta
     3841 ttccggcgca tcaatcggtg gaggaaggtg agttttcacc agagggatta acaggaattg
     3901 atatatttgg taagactttg ggggtagttg gagtgggtgc aattggaagg aatgtgatta
     3961 agattgctag gggtatgggg atgaaagtct tggcagtaga tcttgttcag gatgacaaat
     4021 tggctaagaa aattggtttt aaatatgttg aattggagga gtgtttgagg tcggtggatt
     4081 ttctaacttt gcatgtaccg tttactcaag aaacacgtta tctaattaat aaaaaaaaca
     4141 taaagctaat gcagccaggg tcggtgttga taaacacagc tagaggagct gtagtagagt
     4201 cggaggcgat agtctgggcg ttgaataaca aaatattgtg gggagcagga ttggatgtga
     4261 cagaagagga agaaagacta gaaagtatgt cgacgataat gagtaaaagg gatatttcaa
     4321 aaggtttact tagttttcat ttgttgagag atagagatga tgtggtattt acaccacata
     4381 acgcttttaa taccaaagag gcaattgaga ggataattaa aacgacgata caaaacataa
     4441 atatgttttg taagtctgaa agtcgaaagt cttaaagtct taaagtaaga tgacaataga
     4501 gaaatttgaa gatctgttag tttggcaaaa gtctaaagaa tttgtcgtaa ggataagttt
     4561 ggtgtttaag gattgcaagg attacagttt taaagaccaa atatggagag caagtatttc
     4621 aattatgaat aatattgctg aggggtttga aagacgtggt gacaaagaat ttaaaaattt
     4681 cttaaatatt tcgaaaggat ctagcggtga ggtgagatca atgctgtcac tagcttatga
     4741 tttaaaatac atagatttaa ttcagaaaat tagtttgtcg gatagttgtg ttgaactgtc
     4801 gagaatgttg atgggtttaa tgagaagtat aaaatgactt ttgactttaa gacttttaga
     4861 ctttagacta tgcaatgata cctgtaataa tctgtggtgg agtgggtact aaaatgtggc
     4921 cagcgagtcg tcaaaaaaat ccgaaacatt tcttaccatt aattaatggt aaatctttat
     4981 ttcaattaaa ttgggaatcg ttaagattaa agtttaaacc agaggaaata tttcttcaga
     5041 caaatgcctt gcaggcggaa atggccaaag aacaagtagc agaggtagtg gaaaaaaata
     5101 tttttattga accagaaatg agaaatcaag gtccggctac tggatttgcg gcggcggaat
     5161 tgattaagcg ggggttgggc gatgaaccat ttgttttggt tcaagctgat gtgttgagag
     5221 aacctggaga tagatttatt gaaatgattg aggaaatgga gaagcaggct gtggcttctg
     5281 ataaatatat tacgggagga ttaaaaccgg aatcgatagt aagaggagtg gactacctag
     5341 tgaaagggag gttggtgtca gacaatggtt tggtaaaaat ttttgaggtg gctgattata
     5401 ttgatcggac agaagaggaa aaaattaaac aatatttggg gacagataag ttgttgttgc
     5461 acgccaacca tacaactatg acgccaaata atttgctaaa aatgtataaa aaatacaagt
     5521 tagagtggta tgaacctttg aaaaatatgg ttgaaggcgg gcaggtggag gctgaatatg
     5581 ccaagatgcc taaagcgggg attgaagagg tgacaaagat agtttataag aacggagaag
     5641 ctttagtggt ggaattacct tttaactggg tagatttggg gacatgggaa agtttagacc
     5701 tgtatctagg gagtaaagaa gatgggaatt ttgtgagatc aaaaaaatat gtagcgattg
     5761 taggattgga aaatgtggtg gtggtggaaa ctgatgatgc gatcttgatt tgtaannnnn
     5821 nnnnnnnnnn nnnnnnnnng tcttcgcggc ctttttgtag aagatcatgg gtatcccaga
     5881 atttggttgg gatggtgacg tggttgaagt tggtagatcg gtaaggaatt tctgtgagtt
     5941 tggcggtgga gaatccggga aactcatctt caagtacaac ataagatggt agcggtgagt
     6001 tgggaaagag gggagatcgg tatccttgat aattacaggc aaaccaagac ataatttctc
     6061 ctggctcgac aatgatgctg tggggtgaat tgtccttatc gtccatatca aaaaaatgtt
     6121 taattagttg acctcgtgaa tctactccta ggaaaatcag tcggcctgaa agtggtgttt
     6181 cgcgaaaaat atgtggtcct tcattgcctt gaaatgggct tgagctttgt gcatctggtc
     6241 ttaacaataa ctgggcacca tcgtgttctt ttgggtcttg gccccgatag atgtggggga
     6301 tgcagcggac ttttggtagg ttgtagacat ctgtttgggg gatgaccgtc tgaagttcaa
     6361 ttggttggac gtagacggtt tgaccttcgt atttccagtc acaaggttcg ttaaaacctc
     6421 gtcgcatgtg ggcactacga gcaaaccatt gttgaggttt ggtccaagga tttgtttcga
     6481 gacgcataga ctttatctga caatacgttt ggctttggat aaaacagcag gtctcattct
     6541 tatagttttg tcttcttgat caagaacgtg caattgttca tcgatgacaa tagcgttggc
     6601 gatgctacaa agactgttta atcggccacg agtgttttct tgttttccga aggtggcaag
     6661 ttttttgatt tctgttcgtg aaaatacctc gtcgacgatt atttctattt ttctagtcat
     6721 gtttgacatt ttatagttat aggatgtaaa ctaacagatg atgtccggag aaacagacag
     6781 attaatagtg aaattaaatc aatttggatt agaggttgat gatgctcgta tttaccttac
     6841 tttgttagaa aaaggtgagt cgtctgcttt gcaaattagt aggactttgg gtatagcgcg
     6901 gaccagagtg tacagaatac tggacaagtt gatagataaa agattggtga ttttaaaaca
     6961 agctagtagt ggttttaggt ttatggctga cgagccatcg agaatgaaaa atttgttgat
     7021 tgaaaaagaa gctgagttgc aagatctaaa aataaattta ccggaaatag ttgaaagtat
     7081 gaagggattg atgggtcagg caaagtctgg ctcaaaagtt ttgtattatg aagggcaacg
     7141 agggttgtcg caagtcaatt ggaatttggt aaatgcgacg acagatttgt tgagttatga
     7201 agtagcgact ccagacgtgt atttacccaa aagtgaatca gagaaattaa tggaggagat
     7261 ggtcaagaag aaaattatgg ttaggacgat tactaacaaa acaaagatac tgccttttac
     7321 aaatgtagag ggaatggtcg attactggca aattaaaagt attgataaaa agaaattaga
     7381 aatttttgaa gatgtgttta tctacaacga cgtttataca tggtgtcaat atcggagcaa
     7441 ggaagagatt ttttgtgtgg agatctataa cgaaaaaatg gcaaatatgc agcgacagtt
     7501 atttgagaat atgtggaatg tggcgagaaa gatgaaggta gttagtgtac aaggggaagc
     7561 ggtgctggct taaagtgcct tctttcttga ggtatcggct gtgtgtttga aggtgatagt
     7621 cgaattaagt attcgtgtcc gtttatgatt attttagttc gtctttcacc gtagcgacga
     7681 caggggagta agtagacggc ttgtgtgccg ccaagttcac taggatcagg aatcattcca
     7741 gttatctcca tagtagttat tgtatgtaac tgatgggtat tataactccg gtgttttgtt
     7801 tttgcaaaaa accacttgaa ggtaggtttg atcagtcaaa acccacttta attccccctt
     7861 gagaaaggag tctaataaat ttagattgag agtggttgtg aaaaacagtt atttgagagt
     7921 tatactgagg taattaatat tttttaaaga attatggctg taggtaataa tgcgggtgac
     7981 catttacaac cagatgttgg tagtaaaact gattttagaa ctcaacgatt aaatcaaatt
     8041 agggagatag ttggtgatca gagcactggc tcgtctgaag atgacgaagg ggcaagaggg
     8101 ttgttgtcat ctattgttaa tttggaaaaa gaggacaaag atgcggagta tttttttgcg
     8161 ctggctcgat tcgttgacga ttctgtgcct tcgccgacgg ttgatgatat tacaaatgcg
     8221 gttttggcaa ctgaatttct atatgaaaaa gaattgaacc ctgataacaa acgacctcaa
     8281 aatttagatc cagaaagttg gaaaggatgg ggtgatgcgg ttgaaagatt agaatttaat
     8341 attggtcaaa ctattgtcga tcaagcagat ggagcaggca tggaaggcct agaaattttt
     8401 tatgatagtg atttacaccg acagatgttt gttaaattag ctgatgatag aattttaaat
     8461 gttgatgaaa gagtcgagta cgacacatat ccgagaaaat tgagtattgt aactgaaagt
     8521 gaaatggaga aggctctatc gcataaagta agtgatgagg aaggaaagca acgaagaaaa
     8581 gcgtctgtcg atgaatatac aaggagtgag tttggggata tatagaaata ctagtgtcaa
     8641 acaaaaatcc cccgattgct cgggggattt aaatttaaaa taagaaaatt attttaattc
     8701 aacagtagca ccagcggctt cgagcttggt tttagcatca gcagcagcat cttttttcat
     8761 ggtacccatg tcagcaggac aggcatcaac catatcctta gcttctttca aacctaattc
     8821 aggtttgaat tcacggacag ctttgatgac agcgattttg ttggtaccag cattggtaat
     8881 aacaacatcg aattcggtct tctcttcaac agcagcggca ggagcagcac cggcggcacc
     8941 agcagcagga gcagcagcca tagcgacagc tttgacatcg aatttttctt cgagtgcttt
     9001 aacgaggtca gataattcca ttactgataa ttcagcaatg tcatttagga ttttttctaa
     9061 ttttttatcc atagtttttt aaatttttaa tttataatta ttattttttg tcagcaatgg
     9121 ccttgagggt cataactaat tgagtttggt tgtagcgcat agcatagacc attctctgta
     9181 gaggattctt aagtccacca atgaattgag cgataagagc tgattttgaa ggtagagaga
     9241 tgaatttctt tagttcgtct aaggaaagta atttgccgtt gtaaataccg tatttgaaag
     9301 tagtcttctc tgaagatttg ttgacggcat cgacttcttt tagaggagca atctcatcgg
     9361 tgttgctata gacgatagcg gtaggtccaa ctaattcttc aggtaagctg ataccaagtt
     9421 tgagtaatgc tcggaaaata agagagtttt tggtaacttc gattaatcca ccagtagatt
     9481 taactttgtc tctaagattg gtgataccag cagcatctaa gccttggtat tgaattaaag
     9541 cagcagactt agcttcgttt agtttaacga cgacttcgcc aactttgtcg attttttgtg
     9601 gtttttttaa tttcataaga tgatatttta gttaaaatac taaaaaagcc tcgaaagtcg
     9661 aggcgtagat taaaaatcta gttttagcct cggtcagatt tttatgtctt gcgaccctga
     9721 ctgtcttcgg taggacagat gtgattataa cagaaaaaag gaaagaaggg tctatttgtt
     9781 ttcttgtgtt gtgtctaaac ccaatccttt tacagctatg cttactaaat ccataccttt
     9841 gtgtaaagtt tgattagctt ttactggatc ttttatttta gttgaagcaa tgatagtttc
     9901 tgctccttgt gttgcgacgt gtgcaccaat tgtagcgctg agatctactt tttgtttttc
     9961 catggtggga ttatcttccc ttctttctta aaagtcaagt attatgggct tgaatggggt
    10021 aaaatgaaga tgtgaagaga ataattttta ttttgatatt attatttgtt ttgggggttg
    10081 gtggatattt tggttggcag tggtattggg aaagaattga tccaaaaggg cttagcggag
    10141 ttagtttgaa tttagattta caaaaatatg attttgatag tttgagaaag cgaggtgggg
    10201 tggctagtga aattgaaata ctgggggaaa tagatgaggt ggaaaaacgc cgaaatcaaa
    10261 aatatgattt tacaacccaa gagattagat ttaaaagtga tggtaagtgg gtgagtggaa
    10321 tgatcaacta ctatccggag aagagtagtt tgagtaaagt gataattatg attcgggggt
    10381 atgcagaaaa agtcgggtat tatccagggt cggggacatg gagagtggca gacgagttgg
    10441 ctaaggaggg gtatgcaaca attagtattg attttttggg ttatggcaat agtgatccag
    10501 aatcagaaga tgtactagaa gctaggtttc acaaagtaat tcaagttaca gatctaatag
    10561 agtcagttaa agctttatct tgggtagaca agagtagaat tgggatttgg gcgcatagta
    10621 atggtggaca aatttctttg tcggtattgg aggtgactgg tgagaaatat ccgacagtgt
    10681 tgtgggcacc aatgaccaat ccgtttcctc aaagtttgat tgatacggct gacccgggtg
    10741 aagaaaaaga taaagcgatt gcttttgtaa actctttttt gaaatattat gatggtcggc
    10801 gttatgcttt tgaaaattat tatgattgga ttaaggcgcc agtgttaatt caacagggga
    10861 ccaaagatgt ttggtgtaaa gttgaatggc aggaggattt gcaggataaa ttaaagaaga
    10921 tgggaaaaag tgtagactta gtggtttatg atggagcgga tcataatatg atcccgaagt
    10981 gggccgaggc ggt
//
LOCUS       DDWP01000024            5752 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_16708, whole genome shotgun sequence.
ACCESSION   DDWP01000024 DDWP01000000
VERSION     DDWP01000024.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_16708"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1670 a         1668 c          666 g         1748 t
ORIGIN      
        1 gattgtcttt gtatataatt caagtacgaa aacttatctt tactagtcaa agacacctga
       61 tctgtgtatc tcacctcagc cgtcactttt ttccctactg gcaccgttat taagaaccca
      121 acctctttct tcccataaac tgttttcacc tgaaggttat cacccgaata gatcgtcttt
      181 accccacttt gattatcagt tacgcttact tcagccaaat ttgagcttga cggaatatat
      241 atccgggcat agtttctata atctcctccc ggccaattgc tgtttttagc aatattttca
      301 taattaattt ttaatacccg cgacagcgac tggttattta tatctaccac caaatccatg
      361 ttcctataga taaaataatt agctttattt acccctaggt tcgcttcaac taaatataaa
      421 taatcagcaa aacattgtgc acccacttgt tctgagcccg tcgcaggagc acacctacct
      481 ccatacaaag ttccatccca acccgcctca gccatcacct ttgctatagg gccattgttc
      541 aatgccattt gaatttcatt cctctctaaa agatcaatta ccgtcttggc caattctagt
      601 cgaccttcac tctttaagtt ttttatatct tcaaacagct gttttcctac cccacccaag
      661 aagcttgctt tttgatttga accagggaaa aattttgttt ctgagaaaaa ctctgcctgt
      721 tcatataaat tgtttttatt aattttagtt ttaaaatcag gcacatatac ttcacccact
      781 accccaacca atcccttagc tacagccaag ttcatcccga tgaccccatc aactttcctc
      841 cctgtcgatt tctccaaaaa ccattgaatg tcttttgccg ccgccacaaa atcaggctgc
      901 cagttagcat ctctcataaa ccacttagct tcacccaaga tatttttaat tggctctggt
      961 ggttctacat gaccagccaa ttgaccatca gcctcataga cgtcctttat atcaaagttc
     1021 aacaatcgac cgccttcaaa gctaagaatc gcgtaagagc caataaatcc tcccgtcggg
     1081 cggatctcgc tttcattttg tagcaatatc atatattctc ttctttttcc gtctataccc
     1141 agaaactctg gtaatatttc taccgcccga gcaccagcag acaaagttct tttgttctct
     1201 tccattccac ttcttagttc attaaaccta cctttccatc tcgccgggat ccacccccaa
     1261 tcgccagaca atctggcttg caacaagccc aacacctctt ccgtctcttt cacgtctctc
     1321 tccaatcctc ccaactcctc cttccaatca atttctttct ccgaaaaaac tgcctcattg
     1381 ataacctccg atctcttggc caggtcaatt gcttttttct caagcaatac cccttgatcc
     1441 aatgctttta aaactgtttg atattttcgc cccacttcaa acctatttaa tccccaatct
     1501 ttcaccagat cttcttccga ctttacttta tccaacgtct tctctgccaa agctgccgcc
     1561 tctacgtatt tcttttccaa gataagatct ttaatttcaa aagccccagc caccattcga
     1621 taatttgtat aaccccaccc aatcaccacc aagacaacta ccaaccatac tcccgccaat
     1681 cctcgccacc cccacttcac ccaattaggg ttcaacttaa tctctctttt tacttttttt
     1741 atttctacca ctggttccac tttttttacg actacaggtt cttctttttt ttctggaatt
     1801 tgagatctaa ggtctgagat cttctctgtt ctctcttgca ccaacctcat atttgagttc
     1861 tttaccaaca taggtctaat atcctcatac ttttcgacaa ttttcttttt cttctttttt
     1921 aacttaagct tctttttttc ctccatctcc ggatactctt cagccataat ttcctcaaga
     1981 gtctttattt ttacatcact cacagtctct acctctactt ttggtgaaac gtcaacgtcg
     2041 ggcacaacag gcaccacctc aaacctaatc ttgttctgct tatcttcttc tacaacttcc
     2101 aattttggat ttaggatttc ggatttagga tttactcctt ttggttttct tccttcttcg
     2161 tccactttgg caaagaaatc ttgcaacacc tcttttactc ccgctccaaa acttacattc
     2221 ggcctccatc tcaacttcct ccatcctcct tctatttctt tttcattcgt aattcgtaat
     2281 tcgtaattcg taatttcttc aactttgaac ctcgtcattt ttgcctcttc aatcaacacc
     2341 ttagctattt cctctgaatt aatcttttct ccccatatat caagcacctc accttcagtt
     2401 cccgagagaa aactacttct cattactgct tctaaagcat cttcctccgc cagcaatctc
     2461 aaatcacttt ctctaggtgg caacattaat gttttgtttc ttaccgccaa ccccagagca
     2521 tttattaaaa aacctacttc atctccttcc atccctaccc catacacccc atgtaaatta
     2581 actaccctcc aatttacccc aagttcaacc aaatcttttg cctgaacttc ttgattaaca
     2641 ccaattactg ctagcttaga cccatcctta acagcctttt cccagactct tctgtctccc
     2701 tcaaaatcaa acacataatc aactttttcc ccaacctcac ttaagtcacc ccacgactcc
     2761 cccaactcca ccacctcaat atcactttta cgcaaactct ccaccaattt cttggctaca
     2821 aacttatcag ccccattaac caaggctact ggcaatccca tactcatact tcattttaat
     2881 ttaacgcaag gtgtattcat agagtgttct tgtaggggcg gatcgatgta tccgcccacc
     2941 ccacgcggta ataaatcggt tattttgcgg ttatcgagtt aaaaatgtac atatttgagt
     3001 ttggaacgtg aaaaactgtc attacatcac atgtcaaata ttgattctcg taggggcgga
     3061 ccgatgtgtc cgcccttcat aattaatatg aattcattcc caaaaaaatg tccaaaccag
     3121 cttccgcaag gcttttttaa aatacagaaa cgtaattata ttaagtacac cagtagatca
     3181 ataacgtgaa aaaatttacc cgtcatagct ttagcaaagg tgggccaaaa aaatccccca
     3241 aaaaacgggg gactgtttta ggctttagac ttttgacttt aagactttcg acttttcact
     3301 agaaagaatc ccatcaccaa catatcaaaa aattccgtca acaccgttat ccaagccgcc
     3361 gcaactattc catagtaggg gacaagccat atatttaaac ttaagttaag cactagcgcc
     3421 attagcccta gataaagcat ctctttttgc cctcctcgag caatcaaggt aaagccaagc
     3481 aaatggccaa tatacgacca tatccaagca attcccaata ttctcaatac ccctactgcc
     3541 ggcagatact cttccccacc caacaccatc accatccacg gagccagtac ccacacccca
     3601 accaacatta gtaccgcaaa tgttgatagt attcccaaag acagccagaa cagttctttt
     3661 ttgtagctat tctttcctga caatattggg aatatactgt ttacataaaa ataagccggt
     3721 tgtatcaata ccccatacat cttgtaagct agtccatacc aagccacctc tttagctcct
     3781 ctaaaatgtt gtattaccat catatccacc gctcgatcat aagttgcaaa cacgactaaa
     3841 aacactccca tcggccaact cattttccac aaagacacta gttgttctcg atccaccctc
     3901 tttacctcaa ttttctctat tccacttgct aaccagtagc cgattaataa tgaaattgct
     3961 ctggctacca aataaactac catcaccatc atcaggctca tcgaattgtt tgccgtcaac
     4021 aaccacagca aaaagagtag gggaaaagca gtgtcagcca ccaccttcag gcccattttc
     4081 aatctcactt gaaaaagtat ttcccaaaat cctgccaagg aagttaacca tatcatcaac
     4141 aaagccacag tcgcctctaa tcggacgcta gccaagccac tccagctcca cacaattacc
     4201 agcccaacca caaaagcaat taatgccatc agcaatctta gatagaatat tttcccccaa
     4261 atattttttt cctcatcttt agccacctct ctaatcccaa tcgttttcgt cccaaaatca
     4321 gccaaagaat caagaaaaac aaacattgaa gaaattaata taaagcttcc gtatccttcc
     4381 aaacccagct ttctcgtcaa tatacctgtc gttatcaaac taaccattac cgacaccgct
     4441 tttcccaata tttgcattgc cgtgttcacc aacgatttct tcatcatacc catattctac
     4501 cttcccaata aattatttgc gaagtaaaca atttttagga gcgcagcttc tggagccatt
     4561 tatcggcgaa gcacgcgagt accgcaaaaa ttcgtttaca agcaaataat tacaatactg
     4621 acaacactac cgccaacaac caataattct gtggcaaagt cagccagtaa tgatcaacca
     4681 tccccgtaac tattattatt cccaatatca accagtttct ccttctcatt atctgaaatc
     4741 taatgtctga aatcttatat atcaccagca taattcccaa tatccctaac tccgctccta
     4801 ctaacaaaaa gatattatgc actggctgca accaataaaa actactggct tcttgataaa
     4861 ctggcaaatc agctataaag ttccccgcac ctacacccaa catcagatta tctctccaca
     4921 tctcaactga catcatgcct agcagcaccc tcttggtcaa tgagccagta tcccaaccac
     4981 ccacaaaatc acttatccga taattaactc ccacaacacc caatatcaac atcaacacac
     5041 cagtaattat taccaaatac cccacaagat tctttacccc aagtctcttt ttcttaattc
     5101 gtaattcgta attcgtaatt attaattgca ttactagtaa cgcccacacc attcttgaac
     5161 cagcaattac gattcccagt acactacacc aagttatcac ccaatacatc acccctcttt
     5221 tcttttgcca actctgccac aacatccagg ccaccaacaa gaatcctgcc aaactatttg
     5281 gatgcgagaa tgtcccatat gctctaacca aaccatttcc taaccacgat atctgagcaa
     5341 tacctattgt tgtcaagcta aatcttctct ctcccaacca ataccacact ccctctaagc
     5401 ttccaccttt aaccatttgc cccactccca acaatgtctc catcacaatc cagaagggga
     5461 ttatccaccc caaacacctt ttaactttca aactttctga ctttaagact ttaagactaa
     5521 ttacccattg taatattcgt agccatttat agattgccac ccacttattc cctgctacca
     5581 agatattcaa tccaataaaa atcaccacca ccaagttctc aaaagtaaat atctttttca
     5641 aactttggac ttttagacct ttagacttta agactctgct ccccatccac acaacccaga
     5701 caatatccaa taaatacact accggtgcca gataatcact tcttatccct cg
//
LOCUS       DDWP01000025            5595 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_51121, whole genome shotgun sequence.
ACCESSION   DDWP01000025 DDWP01000000
VERSION     DDWP01000025.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_51121"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    4790..4875
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5074..5157
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5452..5463
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1658 a          841 c         1460 g         1454 t
ORIGIN      
        1 ggatggtata atttgggcat atggctaggt cacataataa tggtgcagaa acatcgggat
       61 tgtcggtagt tgctttgggt ttgactcagt cggtaaataa agaggtaaag cgggcggata
      121 cggggactgg tctcggtgtg agtgaccagg cagtggggtt tgggacttct atgggagaat
      181 ttattagagg aagtagtgat gctaactggt taactaatac tgaagaatct cttgtggaga
      241 tgtttgatgg ttcgggtttt ggaaatgttg gagatttgag aacttggtgt caagataacg
      301 atacgagaga tgatttgtct ttagaggaaa tgggcgcagt tcaggcttat tatgatatag
      361 ttgatgcaat aactttagct acgttggcaa gtacagcaag gatgagtgat gaggtggtgc
      421 aagctttggg gggcaataat ccgaacggaa gatctgttga agaagctttg ggtatcggag
      481 aagatagtcc gttggctggt gtgggagaaa tgatgattga tgtggctatt gttgatcctg
      541 attatgctga ggttttggtg gatttgaatc gggcagttga ggtgggtgat tatcgtggtg
      601 gggtgaacga cgcaatgaga aggatggacc ataaagatct taaagatcag gatagtccgg
      661 agtggaatat gatcgtaaca tatttacatg aaatgagagc tgcaaacgat gctcaaaatg
      721 ggggtcgagg acggccaatt ccggaaagaa gaccaggtgc tagaaatgat agtggtggtg
      781 tagatatgga ggaatttaca tatgatatga agaggagccg gtttactgaa atggagaggc
      841 aaaataaagc aattagatgg tatgacgaaa gacccccggc atggtatgag gaaatgtctc
      901 ctgacgaaag agatttgatt gatattagac tgaaattgtt gggagctacc gcaaataaag
      961 agttcttgag aaatagagat gtggaaaagc ttaggggtaa tgtgccagaa atatctagta
     1021 aagaattaag attgttatgg acggagatgc ctggttttaa ggaaggattg agattttttg
     1081 tacaagactt atgtgaagat tatcttgacg ctgatggtcg tagaatgttg aggcttaaaa
     1141 cgattggagg gccagacatg actcaagacg ttcttatggc tggaggtggt aagttgcagg
     1201 ctgatgttaa ggataaggtt gagaacttta gtcgatacaa gaaaagtgtt gtggcggagt
     1261 tgaaaaaaag tgttcctgaa ataacagaat ctcaagcagt tgaagcggct tcggtggctt
     1321 ggaacttttt gtacatcgga gattcggttg aatcttggga tctgtttaga gaattaaagc
     1381 caacttctat tgtaagtgac aaattgagaa cgatattcca tccacgaata aaagctttgg
     1441 gtaaatgggg tgtgtggaaa gggtcgattc ctcgtggtca aacggtgacg gatgaaggtc
     1501 aagaggaacc ttttgtggag ggtccacttt ctacttgggt gttggataga cttaaagttg
     1561 aacctggatt taaacaaaga cttattgatg gtgatttggc tggtatcttg ccaaacacta
     1621 tggcagtttc aatgattgaa gcgacaaaga ttggtgatac taatatggct atggctctaa
     1681 tggaaaagag tgatgcatgg atattgagta agcttggtga tgatgataaa aatgtgatgc
     1741 aggcccataa cgatatggtt caggctgctg actggttaat gactgtggtt aaaggaaaga
     1801 ttccttacgg tgataaaaac gcaggtgctt ttgtgtcagc ttttctggaa aatacgtcac
     1861 ttattagaca gggtccgaca ttaccaaccg ttggtggtaa cttaagaagt ttggcttttg
     1921 ttgatagacc ggagttttat gcttgggcag ttctgactgc tgttggtttt gacccgaggg
     1981 ataagaatcc tgttttatct tctgcacctt tgggagtgaa agataatgtt gcttatatta
     2041 aggcggatca acttatctct agaataacta tagggtcttc agttgacaga tcacaaatca
     2101 agaagatact tggtatttct agtcttcttg attggaatgc gaggcgtgct tctaatgcta
     2161 ctgatcgtta tcaacgtggt taaggatttg ggtgttctgt aataatatgg tcttgtgcct
     2221 ctaaaaaagt ggggataaga aatttagtga ttacccttaa tcttacctgt tgtttgtagt
     2281 gcgtcagcga ttgttcctgc tatttgccaa cccttgccgc caccagcatc tctggcatcc
     2341 tgagagaatc taccgcctgc ttcaaggagt gattgtccac caaactgagc aggtttggca
     2401 aatgtggtgt taagtccttc accaatagcc tgaccccatg gagatggttt gatcatgaag
     2461 ataaactgag gaatcatttc cggtaatttg ctaagaagca taagagtaga aagtccgata
     2521 gctccagagg cgatgactgc accaggcaat aaaacgccac catcgataag actgggtacc
     2581 catagaccgg tattttgagg ccagttacca tcgacaccgc cactggtggc aagtttgatg
     2641 agttcgctaa cagtaaatat tgtctgacca gcagcaatag tagtaattat tttgcttgat
     2701 aagaggataa agagaaaaga tatggggaag acggctaaat tgctaattac gtccataatc
     2761 caactgttaa aaccaatttt gctgttggga aaagctccga gacctatttc gaggggggca
     2821 agaattatct taaagataag agtggcataa catttgaaaa gacccatcat gaatttgaaa
     2881 agccagataa tgatgcctat gcctaatatg acaaaaatga taatggcgga gatacttccc
     2941 cagccaacac cggtgacaat agcaactggt ccaccaagaa tacctaaagt ggctccgaca
     3001 gcaaatccgg caacaaagcc aaggaaaagg gtggcaaaga cagggacaga tagaacacca
     3061 aaagtagtac caatggttgg ttcaacaaaa ttggcgaaag atgggacgaa ttggccaata
     3121 agtgggattt cgctaacgac attgagaaag tcgttgagga taccaccaac gcgttgaggg
     3181 tagataagac taacagccat gccttgaaga agatagctag catcgatgag aagaccgacg
     3241 atagcgtagg agaaagtaac taaaataaga gtagtaacta attggggaat ggcgttttgg
     3301 atagtaatag ttgcttgggg agatatcttg acacgaagga caatcatgat accaataatg
     3361 acgaaaaaca gggatgaaag tatataagtg gcgtcacgaa agttcttcca gataggaaga
     3421 attggttgta atccataaaa accaacacct tgggcgtagg caggcttgat gcctaaaaat
     3481 tcgtttttta aattagcgag gtattctacc cctgaaacag ctggagtata gaattgacca
     3541 atgatttggt tagaagtacc gatcatacca ccgggtatcc aaaaaccatt gccgctacta
     3601 atttgatccg tgggcacatc accaaaaacg gtcttaaaag caagataagc atgtgatttg
     3661 tcggcagctt caagaaaaga acaaaggttg ttgccggcat cacaggcttc cttttctgtt
     3721 ttgatgatgt ttggtggttc atcttgggcg agtgagcggc taataaggga gaatgaagaa
     3781 agagatatta gggaaatgat ggaaataaaa ataatagtag agaagctagg aagtaggtga
     3841 gtatcgcggc gatttaattt atttaataag gatcgaaaag acatggttta ttttatcatt
     3901 taaataattg gtatgacaga tgaattttaa ttggcgggcc agaagaattg gcggaaaacg
     3961 taatgaacaa tattgttgcc tgggtcgagg gtggtttggt tgtcgatggc tgaggggggg
     4021 taattgatag tgctgtattc tttttggagt tcactgggga gaaggttgtt gagggcgact
     4081 ttgtaggtgg caaaagtctc ggggacacgg acgtcgatac ggaaagtgcg ggtaaaagtt
     4141 cttttaaatg ttttaccttc gtcggtgacg ataccaaaat aggggtcttc tttgatacca
     4201 actagatcaa gggagactct gttggctttg tcgggagatt cgacttgggt gatggcttga
     4261 caaatatcga gaggttcgac tacttgaccg gtagtaataa gaagttgagc gacttctttg
     4321 ttggtaccta agtttccaag agggatagaa gtgtaggctt ttttggcaaa gtcgataaca
     4381 ccgtcgccat cgtaatcagc attttcttcg tcgtcatcga cacggacgag acggacatta
     4441 gaaccgatat cggtaacctg gacggcacgt tgaaaaagac ggcaggcttc ggtaggagaa
     4501 atggatgtac ctttgtaact acttggaact cctccaggaa agggaattgg ttctggagag
     4561 gtaaaacaac tgatgttggg atcgtaaaat atgggttttt tccccgggtc gggaagacag
     4621 gtgttggggt tgaggggaga gtcactattc aaggcaagag caatatcgac caagcgaact
     4681 ggggcgccat taaaagaagt gacagcttcg ttgacaactg aagcgtaggt ttcggtatct
     4741 ttgttagtta gagatcggac agcttgaact agacgttgac ctttttgacn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnncgcga taccagaagt taccgtaatt attgagacta aaccagacag
     4921 tttgttctat tctcttgatt acctgagtac gacatatccc agtgattttg tcacaatctg
     4981 ggtcaaagta ttgggaaata gtgataggta aatgacagct gacgctgtag ttgccaatgt
     5041 cgttactata gaagttgacg tcttttcggg gagnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaga
     5161 atttttttaa attgcattag ggaccgggga ttttaaattc taggacattg gcaccagtaa
     5221 agtcggagat gaattttaga attagccaag agacagtgat accaactaga ccaaccacgg
     5281 ccatggtgag agtagacgag gcagcagcag ctttcttgtc gtcaccgctg ctggtgagat
     5341 attggaaacc cccaagaaca aacataacta agaaagcaaa gccagctagg gtaacgataa
     5401 cttgaaggat gttggcaaaa agacattcta gaccttgaat agtggcaaca cnnnnnnnnn
     5461 nnnaagtaag aagtaagaag aaaaaagaga aagattttct gacagattgc ataactgatt
     5521 atataacaaa aaggcccaga cacatcggtc tgggcctact taaatataaa actattggaa
     5581 ggtaggaacc ttgaa
//
LOCUS       DDWP01000026            5060 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_5791, whole genome shotgun sequence.
ACCESSION   DDWP01000026 DDWP01000000
VERSION     DDWP01000026.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_5791"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1652 a          951 c          968 g         1489 t
ORIGIN      
        1 aaactgaaag aaaagcacgg agtaacttgt ataaatgtta gtatttatat gtatatgaaa
       61 aaaattatta tcccaatcat tctagtttta atcacttttg tcgcctatta tttttatacc
      121 caaacaaaag aggagacaat cgtctcggag tcaattatct ccaaaacatc tcccgataga
      181 ccagcagaaa ttagtggaat tattacttca attttaggta atgagattaa gattgccaaa
      241 gagataaata gagttattct aagtgaagca gaacaggccg ccaaaaaagt gaagatgcaa
      301 tcactttcgc ccgaggagaa ggcagctgtg cgggagtctg aaaaagagaa cttagaaact
      361 gaagagattt cattaattat tccagtaggc actcccttgg taaaaggatc tggggaagcc
      421 aacggagaga tgattagtgc agatattgcc gatttatcaa aaggtactta tgtttcaatt
      481 tggaccgatg gatcagaaaa tatcgaatat atcaaaataa agggtattta gatgaaaaaa
      541 atattaaaaa ataaaaaaat tattattgga ggtatctttc tttttttatt actcatattt
      601 tggtggttta aaagcgtacc agctagatca accgattctc cacaaaatac agccaaggta
      661 gtcaagacag atttgcaaat tgctttttct gtcgacggaa aattaaccat cgatagttat
      721 cagtctaatt ttttaaccag cggtaaagtc gctcaagtgt tagtcaaaga aggggacccg
      781 gtatataaag gccaggcaat tgctcgttta gatttgcagg agttaagtat taacctgcga
      841 caagccgaaa atacacttcg tgataaacag gccattgctg aaaaaattga agatgaggtt
      901 aaagacaatg acactgatga gacctttgct caaagagctt taagaactac cgctcaggca
      961 gccagagatt ccgcctatga cgaagtatta tctgctagaa gagcttttca ggatgccact
     1021 atagtctctc cagttgacgg gatagtcgcc cagatcggtt ttaaagaagg gcaaagtatt
     1081 agtactcaaa accaagactc gtcagtcatt tttatcaaac cagattcaat ctcttttgtt
     1141 gcttatgccg aggaggatga tgtcctaaaa atcagcgatg aacaaagtgt taaggtaact
     1201 ttggacgcct acaataaaga acaattccct tcaaaactta tttacctctc acctctgtct
     1261 gttattgata ataatggtct ttcttcttat aaaataatag ccagtattga aaatccagac
     1321 agtttccgtc tgctggatgg tatggaaggc tctatttatt tcgtcaccaa ggaagttaag
     1381 gacatcgttg cagtagctaa taaagctgtt tatcgggaag aaaaccagtc ttatgttgat
     1441 gttagagaat ctaatggtga tttcaagaag acattagtaa agacaggctt tacagacgga
     1501 aagagtgttg aagttattag cggactatca gtggggcaag aagttcttat ccgtaactaa
     1561 gatgaacaat caaattattt ctctggaaga catcaaaaag agttacttta tcaaagatac
     1621 tgaattttcc gtccttaagg ggatagattt tgatatcaag aagggtgaat tcgtggcctt
     1681 aatgggtccc tcaggttcgg gtaaatcgac acttttaaat attattgggg cactcgataa
     1741 accaagcagc ggtaaatatc atattcaaga cagactagtt tctgatttat cttctgatca
     1801 attggctgat attcgcaata aatacatcgg ttttattttt caaaatttta atttaatccc
     1861 caccctttca tcgttagaaa atgtagcctt accaagtttc tacaccgaac aagaaaatta
     1921 tgatcgagcc aaagaactgc ttagtatggt cggattgcag gataaattta acaacaaacc
     1981 caacgaactt tccggtggcc agcgtcaacg agtggctatc gctagatctc ttattaataa
     2041 tccggaattc attttagctg atgaaccaac tggaaattta gattctatta ccggtaaaga
     2101 aatcatggat ctaattctgt ctttaaataa aaaatatggc aaaaccatac ttatggttac
     2161 ccatgacccc cacttagccc aattaactca ccgtgtcatt tacttaaagg acggtcgaat
     2221 tactaaagaa aaatccgtat gagctcttat aaatttttat taaaattatt tagattatct
     2281 tggaattcac ttcgagctaa catttttcgg tcatttttga ccacactcgg tattatgatt
     2341 ggcaccacca ctattattat ggtagtagct ttgggggaag gtgccaaaaa agatatcgaa
     2401 gcccaatact ccaacatgtc agtcaccacc atccttatta acgccccttc aactgccgga
     2461 gaaccgtcaa aactttctgt cgacgatatt ccattagtta aaacaattag cactgttgcc
     2521 gatgctgttc ctatgctaac cggcaaggta aatatttcta atagtgataa tgttaccaat
     2581 ttttcggttg ttggcactta ctttgacttt gcaaaagtgg ctaatttaga tcttgttagt
     2641 ggacagtttt ttactcagga tgacgaagac aaccacaaca aagtagctat tcttggggca
     2701 actgtagccg aagaattatt tgggacgaga gatccaaaag taatcggtga aagtgttgtt
     2761 ttggggaaaa gagaattcga aattattgga attgctgcct ataaaggtgg tgcttttggt
     2821 ccgattacta tcgacgaaag tgtctttaca ccctattctt cggcttatcg ttatgtcttg
     2881 ggaaaaaccg gaaaattttc cttcaatgta aacgctacta acgtggatgt tctagaccaa
     2941 accatggatt cactgaccag tattttaaga gaatcccaca atttgggacc agaaaatgct
     3001 gatgattttc gtctcaaaga catggggact acggtggcct cggctcaaag ttcttcccag
     3061 accatggctt tactacttgg tagcgtaggg tttattgttt tgttagttgg aggaattggg
     3121 attatgaata ttatgtatgt gactgtttca gagagaacta aagaaattgg aattcgaaaa
     3181 gctatagggg ctaaagatgt tcacatatta acccaatttt tatttgaagc ctttatttta
     3241 actctagtag gatttgtggt tggggcagtt acttcaatcg ccatatatta tctcctgtta
     3301 tccatgggat taaaaataat ttttgtttgg tggagcgtcc tcctatcctt tgctattacg
     3361 tttgcaactg gcatgttctt tggttattcc cccgctaaaa aagcagcttc tcttgatcca
     3421 attgatgctc ttagatacga ataaatcaac ttctagattt ggtaaccata cataccagaa
     3481 aatatctttt ttcacaaagt gatactatta acatagttaa tgattaatct tgccatgaaa
     3541 aattatctca tcctgcccgt tctcttcttg ttagccacca ctccagcttt agccaaaggc
     3601 ccaagtgaag cccaacaagg gcaacagata aataccacca gtggtagtca aaacaccgtt
     3661 actaatacat cacctaccaa aaaacctatt gtttcaccta ctggtaatca ggtcaaaaac
     3721 accaatgtag ttcaaacaca aaaccagggc gaaactactc aactaagtgt taagactcaa
     3781 gagtctgagc agttaaatca agctgttgat gatagtctga ccaaggtctc cgataaagtt
     3841 cttgagctaa tcgatactgt tggtgctaaa ggtggcattg gtcaacaagt aaaagaagtt
     3901 gcccaagaac aggtaaagac acagcaagag ataaagtctg cctttactca gctaaattct
     3961 caaggagcag ccaccaagtt cttttttggt tctgataaaa aagttgtcaa atcaatggaa
     4021 cagaaaatag accaaaatcg cttaatgatc caagatcttg aagaattaaa gttacagaca
     4081 aaaaatacct ccgacattca acagctccaa gaaactattg acctaatgat ctatcaaaac
     4141 acttctttac aaagcaaagt tgataaagaa aataagtcaa atggaatctt tagctggtta
     4201 actaacctct ttagtagata agtatttttc ttgcattcca gacatgaccg aaattgaaat
     4261 tagaggcaaa ttatcacaag gtgagtttga gactttgttt aaattgttaa ctagcaaagg
     4321 ggagttgacc gaccattatc atcgtctctc tatcgacatt tctcccggct ttgatccgac
     4381 caccaagacc tggcacaaca ccaccggcct cgatattcgt ctcaaaaaaa gtgacgacaa
     4441 agagaaaatc tctattaaga ctggtagttt cactgatcta gagagaaaag aagttgaggt
     4501 caagattcag ccaggccaat ttcttaatgc tctcgatctt ttagaaacta tgggttacct
     4561 ttcaggtatg atttacctct ggcaaagttg ggaatttgac taccttggta ctgaggtcaa
     4621 attatcaaaa tataccgacg attattttac ttttgaagtc gaaggtaaag ataattcaga
     4681 tgtccagtct atcgcccaag atctccattt aacttcatat actcaagagc aatatcatcg
     4741 ggctatcgat tgggaaaacc agcacattca ccaattatat tctcgagaac tagccgagaa
     4801 attattgaaa agtaattttt agttagtcta gcggatttat ccgccgtagc gagtttgagc
     4861 gagcgtaggc ggagggtaaa ggatttgaac ctttgcgggc tttcacccac acgctttcga
     4921 ggcgtgcgcc ataaaccact cggccaaccc tccttataaa gtcgtggaac gtaaaatttt
     4981 attcataaaa ttaacgttcc aacaacgttt aaattgcagg gcaatttatt acgttgtgcg
     5041 cctaccagga atcgaacctg
//
LOCUS       DDWP01000027            7071 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_29372, whole genome shotgun sequence.
ACCESSION   DDWP01000027 DDWP01000000
VERSION     DDWP01000027.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_29372"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2138 a         1423 c         1260 g         2250 t
ORIGIN      
        1 cgaccgatca caatcttcgc agtctcgagt tgagtcaaga gaagcttggc caaggagatt
       61 gtcttttaat ttttcctgag gggattatca ctggtggtca aactagtcag attatcaaag
      121 gcgggacggg ggcagtccgc ctgtctttac tttctcatgt tccgattatt cctattggta
      181 ttcgggggag caatcatgtt catccctatt tattaaaaaa cagattgcca ttttatttca
      241 aacgccatct cccgattaat ataaaaatcg gtaaagaaat taatttccca acacatcccg
      301 gtcttgattt gtcttctcac actgattcaa atcacactct tctccgttct ctcactgata
      361 aactaatggt caaattgtct aagctaagtg gattaactaa tactgttgtg atatcagtat
      421 aaagatatta caatatagtc ttcgatgaga aatttttacc atcttttaat caattctgtt
      481 attagctcga tcaccaactt tactgtttgg tttgctatta ctttcttctc ttatctccag
      541 acacagtcag ttttggtcac gggccttatt tcaggttttt atctcgtggc cacagctatt
      601 tccggttttt ggtttggtag tttggttgac aatcatcaca agaaaaacat catgttgttg
      661 tccagccttg tttctttaat ttcttttagt gttgggttag tcatctatct cacagccgca
      721 ccaggatctt ttaccaacca gtccagcccc actttatggt ttcttattat gactctcatg
      781 gtaggaattg tcgtcagtgg tattcgcaat atcgctctgc cgactatcgt cactattatt
      841 gctcccgagc ccgagcgcga caaagccaat ggcttagttg gcactgcctc aggtatttct
      901 tttctggtta cctcagttat cagcggtctt ttagtcgggt cttctggcat gtattgggtg
      961 ttgattttat ctataatttt ttctcttttg actatttttg atctttggtt tattcctatt
     1021 cccgagaaca atattgtcca cactgccgag catggccaga aaggtgtcga tatcaaagga
     1081 acaatcaaaa taatttcttc cacccctggc tttactgctc ttattttatt tactactttt
     1141 aataatttct taggtggtgt ttttatggct ctaatggatg cttatggttt gtctctagtt
     1201 tccgttcagg tctggggatt attatggggt tttcttagta ccggatttat tatcggtggt
     1261 ttaattattg ctaagtttgg tcttggtaag agtcctatca agactctttt tactgccaac
     1321 attattattt gggctatcag tgccattttt actatttacc cttcgataat tctcttgact
     1381 attggtctgt ttatttacat taccgttgtt ccctacatcg aagcttcaga gcagactatt
     1441 attcaaaaag ttgtccctgt cgagaggcaa ggtcgagtct tcggttttgc tcacagcgtc
     1501 gagcagtccg cttcacctct gacttctttt ctcatcgggc cattgactca atttgttttt
     1561 atccccttca tgactactgg tgcaggagta gatttgattg gtggttggtt tggcactggc
     1621 cctggcaggg gaatagctct agtctttact gttgccggaa ctatcgggct tattgtcacc
     1681 atctttgcca gtcgctcccg ttccacccat cttctttccg ttgcctacga gaaccacggt
     1741 aagtagtttt tcagaaaagt actataatcc ccttagatgg atataaaact ttacagatta
     1801 attctatgtt ttcttttggt attttttata ttcccgagta ctgtttttgc tcatcccggt
     1861 cgcacggctg ctgacggttg ccattattgt cggaccaatt gtgacaaatg gggagtttcc
     1921 tggaatgaac gtcattgtca tggtggtggc gtcgcggctc cagttgaaga accaccagtt
     1981 gtcattaaaa aagttgtccc cactcgcact cctcccactc gccgaccgac tttagcccca
     2041 actagaaaac cgactctaac tcctaccaag aaaccaacca acactccaac tttaaaacca
     2101 accttagcgc ctactttaga gccaaccaag attttttctc caacttcggt tccaacttta
     2161 gtaccaacaa ttgttccatc ctccgcttca atttttcaac tcaaggacga aacaaaaaca
     2221 gtccaacaag aaaatatatt ttcaagattt tttggttggt tttttaagaa aaaataaata
     2281 catggaagtc aaaactaaaa tcattttaaa tgattttttg agtaaaacat atctaaagga
     2341 tcaagataat ccattcatta cccaagaaga cttaaagacc aatatggata gataccttct
     2401 tctttataca gaatttgaag gagagttggc aacattttca gcatattttc attaccgtat
     2461 taacactttg tttgaatttt ggaacacaaa gtttaaaccc actacaggag gctactttaa
     2521 tgctgaccca agtcgagaat taattttatt aatagatagt taccgtgata tttcagaaaa
     2581 tttaaaaaac tcagaagacg agattactat cttagataat tatcttgaaa ctatggacag
     2641 tattcttcca ttattaagca gtagtggagg gtcaatcatt ccgatagact ttggaaggat
     2701 aaggtgtatc aaaaaatatg atccaatttt tatattgaca aaaacaaaca ctagtcaaat
     2761 aagcagtaac acttcttata aacttaattt aatcggagaa ggctcttatg caattgttaa
     2821 gaaatacttt gatcaacatt atcaaagatg gtttgcaata aaacaggcta agtcggattt
     2881 aaccgaaaaa gaaatacaga gattcaaaaa agaatatgat attttgtcca aaataaaatt
     2941 cccctacatt ttagaagttt tttcctacaa tagtaaaaac aattcttata caatggagtt
     3001 ttgtgacttt tccttgaaag attatatcat ttggaataat caaaaactct cgtttgctat
     3061 aagaaaaaga atggcgctac agtttttgta tggagttgac tttttgtata aaaaaaagtt
     3121 atttcatcgt gatttaagtt ggaataatat tttagtaaaa ctttatgatc aaaatattat
     3181 ctatattaaa atttctgact ttgggttggt taaagaagaa gactcagagt taacagacag
     3241 tgatacaagc ggtaaagggt caacttttca caaagatcca ctattagaat ctttcaagga
     3301 cgtaagaatt gaaaatgaaa tttattctgt agggtgtctt ctgagtttta tttttacagg
     3361 aaaacaaaac ctaaataaga atcaattggt agtgggttta gacagtatca ttgaaaaatg
     3421 tacagataat aaaagtctag aaaacagata tcacagtatc accgaaatta ttgatgatat
     3481 taataaaaaa atcattacat aataatcatg actacaatac atatatcaaa acttgacgcg
     3541 gcaaaaaggg aattagaaca tgccattaga ctcttctttt tgtctggtga tccggtagtt
     3601 atccatttag taatttattc atcacaacaa atcttacgag atttagctag aagtaaaggg
     3661 ttatcaacat ttattgatga aatgatagat agggtaaaac ctgagtataa aaaatacctt
     3721 aaaggcaaac tcgcttcatc atacaatttt ttccatcatg cagataagga tcctacggat
     3781 atcaaagaat ttaatccaga gattaacgaa tatgcgcttt gggaatgtgt tgacctatac
     3841 tcaactctta caaaggaaac aacaggtatc atgaaggctt ttagattttg gttttttatc
     3901 aagaataacg gaatgttact tgagcaaaaa gacaaagagg tctacctgga agttgcaaaa
     3961 aaagttgatt taaataataa agccgaattt cttgagcttg ctgaacagct tgagaacaga
     4021 aggactgtat aagttctcag ttatgatgac atatccgtgc taaatctatt aagtttttcg
     4081 atactcacat atataagtat cttgtatact tctctcatgt cccctctaaa attcataaca
     4141 accctcggag agccgagcgt agaagataaa caaaaattag tcgaaggtat gctggcgcac
     4201 cacgccaaaa atggtcacat cagaaagact gagactatct ctatttttct tcacgacgaa
     4261 aatgaccaag ccgtcggtgg agtgatcgtt tcttttcttt ggggtggtat ggaaattcaa
     4321 gcactttggg tagatgcatc tatccgtggt cagggttggg gaaagaagtt gatggaagcg
     4381 gccgagcaag aggggataaa aagaaattgc accattgctt ataccaatac tttttcttgg
     4441 caggctcctg atttttatca gaagatgggt tatacacttt atggccaatt agacaatttt
     4501 ccagaaggga atactctaag ttattttcaa aagaaactcg ctcctgctga tacaattaat
     4561 caatgactga aatagaaatc tttgaacaac tgtttgagct aggcaagacc gcccaagatc
     4621 cagaaggggt cgttgttgct tgtttagttg aagatgacaa tgtcatactc tcctctccca
     4681 gtgctaatga tggaattaga cacgccgaag atttactttt agaacaggca aaaatatcag
     4741 gtatagacat caacgacaaa gtgatcctat atgtcactct tgagccgtgt agttatcgta
     4801 gtccaaaaaa caatgtcgat gattgcacca ctattattat aaaagcaggg ataaaaaata
     4861 ttatttatgc cgcccctgat ccagaatata gccaagaagc ccgagacaga tttttaaaag
     4921 caggcgtcag ctaccaaatg acccaggaca aagacataac cctaaaggct attaatctgt
     4981 tcaataacac tattactatt cctctaacct caatgaatct accccggaag aaaacaattc
     5041 ctcaagagtt ggagtaagtt aacctgcctt ggataagatc attatttata taactaatcc
     5101 ggtcaactgt ctgtaaacta catccctctc accaacccca tcagtatccc ataaagccag
     5161 tttactccca agataaatcc attcactatc cacccgatca ccatcatcgc tactcttaac
     5221 acttcttcaa tcattctcaa ttataccgcg atataatccc tcaatggata tcattcgtat
     5281 ttttgtcaat gaaaaagggg agtatggcaa ccctcattgc gttgtcatcg ataatgaaaa
     5341 aaagttagac gatcaaagtc gacaagcttt tgctaccaag atgggcttaa gtgaaactgt
     5401 ttttgtcaac gataatgcta gcggtaatgt cagtattttt agtcctattc gtcaatgtcc
     5461 ttttgctggt agtgctcttg tcggtacggc ctggttactt aataaattaa acaaaaaacc
     5521 tctcgatttt cttttatcta atgatgtcaa ggttcccact tggcaagaag gaggcgccac
     5581 ctatattcgc atggccaaat ctattttgcc caattggaat tatgaacaac tatcaactcc
     5641 taccgaagtt gaaaagttga ctttggctga tacaaaaaac aaagaacata tgtttgtttg
     5701 ggcctggatt gacgagggaa aaggtttagt tcgtgctcgt acttttgcct ctgattgggg
     5761 tataccagaa gatcctgcta atgggtctgg ttctatgaga ttagctgatt cggtcggaaa
     5821 agagttaacc gttatccatg gtcttggctc gattattcac gcccgaccag tagaggacgg
     5881 ccttatcgaa gtcggaggat tagtctctaa ctctcctcac aatacttttt gaaattatcc
     5941 atgatcgcct gccagccttc tctctgtttt tcctcggggt attcagtttc agtttcaaat
     6001 gtctgtgtca cttttgttga ctctttgtca attgggttaa aaactatact cacttttctc
     6061 ccaccttcca tctcgtattc aattaattta tatagcttaa ccgcggtgta aacaccatta
     6121 aaatcaaaag aacggctccc atcttttgcc gccataatat aggaaaactc tccgcctgtc
     6181 gccaattcgt ttgtcgcctt aggacactcc cagtcatcac tggcatgatt ccattttttt
     6241 acactttcag ccgcagtcca attcttccat actttatcaa ctcctcgatt tactaccact
     6301 tctactgtaa tcattgccat attgtttttt aacaattaac taataccatt atagtaatag
     6361 aataaaatca aatcaacgat atatcaaagc cataagcagt ataattaact atgtctccag
     6421 aaaatcccac cctaacttct tcaccaattg aacaaatgtg gcgttcaacc atgaatgcac
     6481 ccctgacact agaaatcctg aaacctgtcg ttcctcaaat gcgacaagaa ctaatttctg
     6541 taggtgtcga tctatcaccg actccagaaa aaatctcctc cttagccaaa aaacaatttc
     6601 gtgacctaga gactaatcgt ggcgttgggg tcgatcttgc attgagacaa gccaaggtta
     6661 atgggggtga gttactatta ttttgcgaca agcggtgtgc agactctcct ggtgtaaaga
     6721 atgtggatga ctctcgaggc attggtcttc gtcaagtttc tgctgactta attcaattat
     6781 tgattaatga aagtcccagc gacgggcaaa tccaaaaatt tgttttgcgg actaaccgta
     6841 gaataaaaaa agataccgag cgaaaaacat gtcaggcacc tgagccagtc aaagttgcgt
     6901 atagtgacat ccttaagatt actcccgata aaaaatggca tgagtttaaa tctattccct
     6961 acgactcctt acctgatctt tggtcttttc tcgtcggaaa gaataccgag tccgactcgg
     7021 cggtataatc aaccatgaac caaccacttc taataatcat ctctggtccg t
//
LOCUS       DDWP01000028           18246 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_31071, whole genome shotgun sequence.
ACCESSION   DDWP01000028 DDWP01000000
VERSION     DDWP01000028.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 18246)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18246)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18246
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_31071"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         5419 a         4578 c         2539 g         5710 t
ORIGIN      
        1 ttctgttttg acagaatttc ttcacttggg ttaatcaata ctgattcttt tttacctttt
       61 attaaaacgg ttttttggtc gactagtttt atttccatag ctaattttaa cattgaaaag
      121 actttgatgt ggtatcatct ccattaaact acatgctcaa agaactattc atcgcactaa
      181 ctctaggcgc tctattaggt tttggcctaa ccggcggcta cctagccatt aaaaataacc
      241 aacaagcttc cccttcttct ttgggcacca ctactactgt tgttattgat acccctccgg
      301 ctgatcctgc tgatacctcc gcatcttctg ccaccaatca acagtcagta gaagacacca
      361 gcaaccacca actcaccatc gactcaccag atcatcaatc tatatctact agctcaaaaa
      421 caactgtcaa aggatccacc actcccaata gtataataat tattagcacc atcaaacaga
      481 caattcatgt ttcttccgaa gcaaacggct ctttttctca agaaatcgac cttgaaaatg
      541 gcgccaatat tattcaaata gactccatca gcccaactga tgagatagct actaaccaac
      601 ttctggttac ttattccacc gccaaattct aatatgacat actttttatt tttaattctt
      661 gtctcctcca tcctgaatcc ttccaaagtt tttgcccaaa ctcctactga aactgctgcc
      721 aacaacattc aagccatccg cgaagctgtc caacagaaag tcaaagacaa acttaaagag
      781 atcaccagcc ccagccaagt caaaaaatct cttattggga ccgtcattca gattgattca
      841 tcctcagtta ccatcgaaca caaaggctcg actcaaaaac tgatgatcga atcagacctt
      901 actctagtaa attccctagg caacaaggtc actctcgata aaattaaaat aggtcaagat
      961 attattgcca tgggtattga cgatgcctct tccagtacgt ttagtcttaa aaggatagtc
     1021 ttaattgatc taaagaaaat caatttctcc aggactattg tagttggcaa gatagtagat
     1081 atttcaaaaa catcctctct tcttactctc gtttctgatc gcaacaaaaa cgatttgtat
     1141 cagataaaga ctgacaccaa aaccgaactt gtcaataaaa ataaacaaaa aataaaatca
     1201 tctgatatca gtcttggtaa aagagtcatt ggtattcttg tcccagatga aaaaatgagc
     1261 aagacctatt atgccaacaa gctaatcagt ctagacttgg cccctgaagc ctctcctacc
     1321 gccaaaccat aaacttgaca taaccctact tattgctaca atatcctcaa tgaatacagc
     1381 agtcgtgatc ctaaaccatc accataccac caactagttg gcggtaattg ttgtatcaaa
     1441 acaagcaccc gccaagagcg ggttttttta tatctggtgg tattagctca attggttaga
     1501 gcgcagctct gtgaaagctg aggttgccgg ttcaagtccg gtataccacc ccaaagtagg
     1561 aacagacctg tgtgtctgtt cctctaacaa aaatatgcaa aatttaatat caaacaattt
     1621 acatcacacc cataccgtgg cacaaaacca acggggtgtt ttcgtgtctt aaaactttat
     1681 taaattatct aaagccccgt aacaggggct tttttttgga tacgaaatac ccaatccgcc
     1741 tctggcagat ccccaattat taaaaaattt atttataaaa atatgacaga aagaaaaaat
     1801 ttcattacaa atattgtcga caaaactaaa caatttgttg ccgatataaa aacacgagag
     1861 caagaaagaa tcgcccgtga aaatgaatgg tgggaaaaat atacaactac acccccagca
     1921 gaaggtgaac cattaaaaaa gtcctatcag gaagatattg gcataatggg tggatttaaa
     1981 cacatcgatt tcgaatggga ttcagaatca aaaaagtggg ttaacgttgg ccaatatgta
     2041 gaatcaggtt acggacctca ctaaaaagtt tattatttgt ttaaacttaa catatgcaaa
     2101 acattttaaa cctaaccaaa caattagttt ctatcccctc ctgggttaac ggatcaacca
     2161 acgaaataaa actagctgat tttatttttg attatttaaa agataactca acactaagat
     2221 taactaagca gctcgtcgtt gacggtcgct acaatatcct cgctagtaac ggtgaagata
     2281 ttcaaacctt aatcattggt cacaccgaca ctgttggtgt cgacctaaat gactggaata
     2341 ctccaccgac cacacccgtt attaaagcag gtcgcctcta tggtcgtggc actaccgata
     2401 tgaaaagtgg cttagccgcc atgatgattc tcgcctgtca agacaatttg ccagacaata
     2461 ctggttttct gttctacatc gacgaagagt acagctttct tggtatgaaa aagtttatcg
     2521 ccgactatgg caacatcatc aaaccaagta gaattatatc tcttgacggt agcgagctag
     2581 agatcagtaa tggctgccga ggcctgatcg agattgagat agaaattact ggtgctagtt
     2641 gtcatgctgc taccccaaaa aacggtatca acgccattaa taccagcatt actctctgtc
     2701 aaaaattaga aaaacaaatc aaaacatcgg taaacatcgg attaatcaag ggtggttcgg
     2761 cgccaaatgt cgtggcggaa aaatgtctat ttactatcga cattcggcca gatgatcaaa
     2821 atctaactgc caatatagtc agacaaaggg tactggatat tgctcgagag ttagaagtca
     2881 ctgtatctcg attaactatt aattatgact ttggttcttg gactactcca attagttcta
     2941 tcccttcact cgggttgcca tttaaaaaca tcaccgagtc aggttatata gatatccagc
     3001 tactttggga gaagtttaat cgaccgatct gttttaccat cggtgctggt tgccaaaaaa
     3061 tggctcacag tgccaatgaa tatgtggaag tagacaagtt aattgagctt cctcagatgc
     3121 tgacagatat tatcaataat atctaactat ctcccggcag cccagctgcc gggtaagaaa
     3181 aattataatg ttacaatcaa attatgaatc agaacaaagt acaaacactc aaaggtttcc
     3241 gtgatttttt gccggaaacc atggtggtca gaaattatgt caagaagatt ttagtcgaat
     3301 cttttgaaaa atttggtttc gagccactcg agactccagc tttagaatac gccactaccc
     3361 tcatgggtaa atacggtgaa gaagctgaca agttagtcta cgcttttact gatcaaggtg
     3421 accgtcagat tggtctgcga tacgacctga cagtacccac tgccaaggtc ctggctatct
     3481 atggtaatga gctacctcta ccatttaaac gctaccaact ccagtcagtt tatcgagccg
     3541 aaaaacccca aaaaggtcgc taccgagaag tccttcaatg tgactgcgac accttcggca
     3601 ccgactcacc tattgctgat gctgagatca ttgctgtcat ctatgaaata cttactcgac
     3661 tcaattttaa aaaatacagc atccgtataa atagccgaac agttcttcgc caaatactca
     3721 ccgattccgg tatcgaccaa gaccaaaata ccgtacttca atctctcgac aagttccaaa
     3781 aaattggtca agatggagta acaaaagagc tcatctctaa aggcctagat ataggtaaaa
     3841 ttaatcaaat ttttgaactc attaaaagta tcaaaccaga tcaagagctc caacaggtct
     3901 tcgatcaaat caagctcctt ggtgtccccg acaccgccta tattttcgac cccaccatgg
     3961 ttcgaggtct agattattac actggtacta tttttgaaac ctatgtcgaa gaaccaaaga
     4021 ttggctctat tactggtggt ggccgttacg acaacctagt tgaagtcttg ggtggaccaa
     4081 atattccagc agttggtaca actatcggcc tcgaccgcat cgtcgactgt attattgaat
     4141 taaacttgct cccagatctt caaaaaacca ctactaagat catgatcgct aattttggac
     4201 aagacgaaca atcactcaaa ctcgcttcat tattaagaca aaataatatt aataccctcc
     4261 tctaccccac cgccgagaaa ttaggtaaac agtttaagta cgccagcgac aaaaatatcc
     4321 cctttgttgc cgtcatcggc cctgacgaag cccaaaataa tcaagtaact atcaaaaaca
     4381 tgtccaccgg tgaacaaaag acaattgaac aaggcgaaat tatcagttct ctgctataat
     4441 ctactactat gaaagctaat atacacccta cctaccatga tgactgtcag gtcaactgtg
     4501 cctgtggcaa caaatttact actggttcaa ctctaaccaa gattgacgtc gaagtttgtt
     4561 ccaaatgtca ccctttcttc accggtcaac aaaaattcgt cgacattaag ggaagaattg
     4621 acaagttcaa agagaaaatg acccaaggca ccacctataa agctgccaaa gccaagcaaa
     4681 ttgctgataa aaaagccaag aaagaaacaa attagctagg aggttttatg ccaactacta
     4741 actcaaatat agccctatta gaattccgtc ccggtcctgg tggcgacgag tcaaccatct
     4801 ggatggatga tctgatgaaa atgtactccc gttatgctgg caaagtcggc tggaaagttc
     4861 aaattctcga tagcaatctg cttaaaatat ctggtatcga ctgttttaac cagcttaagt
     4921 gggaaactgg cactcaccga gttcaacgtg tccccattac cgaaaaacac ggtcgtatcc
     4981 agacatccac tgccgctgtc gttgtcatgc ctcaagttac ctctcatgaa atcatcatca
     5041 atcctgacga agtcgaaatg actgcttccc gagctggtgg aaatggtggt cagaatgtca
     5101 acaaagttag tactgccgtt cgcctactcc acagacctac cggattaact ttttcagtta
     5161 ggcaagagcg aagccaagaa caaaatcggg agattgcttg ggacttatta cgtgccaaat
     5221 tatggcaaat tgaggaagac aaacgccttg ccgccaatgg ttctactcga gacaagattg
     5281 ggttagctat gcgtgctgac aagattcgta cctacaatta ttctagaaat caaatcaaag
     5341 accaccgtat caacaaagaa ttcaatctaa ctaaatgctt agacggcgat atcaccgaca
     5401 tgcttcttga gctcaccatc ctcgatgaac ctgaaaagac tgaaaactaa ggtaaaactt
     5461 gacaacataa tattataggt ataaaattcc tcattatttg tatacgtaat aaatatgcca
     5521 caagttggaa aaactgtcga aattgttggt gataatcaaa aagtccatgc cagaattatt
     5581 cgactcaaac ttgagagtgg caacaccgtt ctaacagcca tagacattgc caaagggtca
     5641 caacctatcg tccgagacat ctttggctta attgctcacc aagaaaacga gaaaaaacaa
     5701 ctaccttttc ccacaaccgt aagacagact cttccagatg tcgataaact aattggagtg
     5761 gtcaaatacc tcacccctgc tgatactgat tacgaatggg caattaaaag cgttattgac
     5821 catggtgatt tatccacacc agttgtggcc cgaatcacca aaccattaaa tagacaataa
     5881 aacacaatat aataattgct ataatcctgg caaattatga cagtcatctc aattggacac
     5941 cgaccttctg gcaaaagctc tgaacactcc tctcaaccaa gagctagtat tactaggcta
     6001 gtccccgagc tcgaagaaac acttcacgaa acctgtcgtc tacctgtcag accagaacac
     6061 gatatctgtc ctcactgtga cgcagtcctc aagccagcaa ctcaagagat aaaagtagct
     6121 ccagtagttc aagttagaag aacaacttta ggttcagctc caaataacac acccgcccca
     6181 gtcaccagag aaggcgcaaa ctatctcagt cgcaaccgaa ataggcctat tcgccccacc
     6241 ggcaacaaaa gtgttaccga catccactaa aacaagcctt tgatacacat ctcttgcata
     6301 ttcaaagtcc tatagtataa taaatatatg gatacactac caataactcc actcccctgt
     6361 gtcctcggag gcagcgattc ttcacgtcca ggagaaatgc cagaagcaat cttaaaaaga
     6421 agaggtcaac ctttaacact caaactagaa aactttccaa gtaatgaagc ttaccaagct
     6481 tacatcaaaa aacacgggaa agaagcacta gttgttagaa caagataata ttacaactat
     6541 gggccaaaca gctatcagta tcggcaatgg ccgaccagag actcctccaa gcggaaacac
     6601 caaaattcct ggaggaaaaa cactagatca ggtaagacaa gagtatggaa agccacaact
     6661 caaagctcca gctaacattc ccactattcg ccaatcattg acacaaaaaa gacccaaata
     6721 atctagctac tccctcttct ccaacctcac cgaaacggta atatccttat tctcccgata
     6781 cacttttaag ttaagcgtat caccaatttt ctttttgtta atataattag caatcgagaa
     6841 atcctcaacg attttgttcc catcaatttc tgttatgata tctcccactt ttagttctgc
     6901 cttttgagct ggactaccat ccccaatttg tctaacataa gccccggccg gcacctcatt
     6961 caacaatgcc gcttgtttag atataatctg atatccaacc cctagataag gcctatcaaa
     7021 ctcccctgtt gtcttaaagt tattaatcga ctctttgacc atattaattg gcaaagcaaa
     7081 accaatcccc tgtccatttt gagacaccgc cacatttacc ccaatcactt caccacttga
     7141 attaaataac ggccctcccg aatttcccgg gttaatcgcc gcatcagtct gaatcacatt
     7201 acttaacttt tccgagcctt caagtgggct accagccact atccctcgcc ccagtcccga
     7261 gataacccca gttgtcactg tcgatctaaa ttcacccaaa gctgttccaa tagcgatcac
     7321 tgtctgtccc acttttattt tttccgaatc acccaatgtc actggtacta aattatcttt
     7381 cttaactttc aatatcgcca gatcattctc cggatcacga taaatttccg tcacttcgac
     7441 cacttcgtcc ttaccaatca ccactttata tttagcgcta gtatccgaca caacgtgctt
     7501 gttagttacc accaagccgt cagccgaaat aataaatcca gttccgatat ctttttctat
     7561 tttttcttca gttggggctg tcccaaaacc tccaaaaaaa tcaaaaccag caaaagggtc
     7621 aaacacctta ttaattttac ttatcgatac agttactacg cttggagtag ccttctccac
     7681 cacccgagtc accaccgact cctcattgga cacctccaga gtcttctcca ctcttcctac
     7741 cctatctaaa gctttataag tcaaccatcc cccacccccc atcaaccccg ctccgatcac
     7801 tacaccaata aaaatatata ttaaattttt actattcata aattcaatta tattcaaaat
     7861 gactattgtt tcaacaacac ctctatcgct ttatcaagct ggttatcagc tttggagcct
     7921 tcttccttgg gcggttcata gacgacctcc acatcaggag acacacccac tttatgaata
     7981 tttactccat taggtaacaa ccatctagca atagttacat gaattcccga tccgtcggaa
     8041 aaatcttccg gttgttggac agttcctttg ccaaaagtct tttcccctac caaagtcgct
     8101 ctcttatact ctttcaatgc tccagccaat atctccgaag ctgaagccga gccaccattc
     8161 accaacacta ccactttagt atccaccaat cttcccaaag tcttatccac cacataatct
     8221 tccttggaac catcagacga ttcctgcgac acgactattc cactctttaa aaaatcagaa
     8281 gccaccatca ctgatgcttg tagatatcca cccggattat tacgcaagtc cagtactatt
     8341 ccagcagtat tttctttttt aatatttgtc actatctctc cccattcttt ataaagcaaa
     8401 tctgtaaatt tatacaactt tacccacgca actcttttcc catccttctc tttaaattct
     8461 agttcaatac ttggcactac aatattttct ctttttagag ttacatcaaa agtttccttc
     8521 gagctttccc gatacatgtt taaagtcact tcagtaccta tttttcctcg gattatttcc
     8581 accgcttctt gtaggcttat acccacagta tttttctcaa tctttttatt tttatcagtt
     8641 attttaagta tcaaatcacc cgactgtaat ccagctcttt ccgccggagt cttagccaaa
     8701 ggtgccatta ccgataaggt tttatcttta taacccaaac ttatgcccac tccaccaaat
     8761 tgaccagcca aatcctcatt ggcgcttttg ttctcagccg caggtaaata aaaagtataa
     8821 ggatcttcca aagaagacac cattcctgag atagccccct ccgccatttt cttatcatca
     8881 aatttatctt tctctaaaaa caacgtctct aatctgccct taacatacca catttttgac
     8941 aagttaaatt cactggtgcc ggtcaaccca aacttattac tgctattctc tccaaggcgg
     9001 taactaatta aagaaaccag cgacaacaaa gataacaata agaaaacatt cctaatctta
     9061 cgcattaaaa aaaattaaac ttctaagaaa agcttatcct gatctttatc aatagaaact
     9121 ttttttactc cctccaagtt gctagaaagt aatttattaa aatcagtttc taataaagaa
     9181 atgatcggca aactgatttt aattctacca tcattcaaat cacttggacc atttttggta
     9241 attattgcca ccgttcccac tacctctgcc tttaatacag ttactgttga caatttttcc
     9301 accaccaata ctttcccctt atcgcttgat gataaatcaa aaaaactatt taccagacgc
     9361 actccggcag atgcagatag agaatcaccg tacaacaaca ccccatcaaa ctccagtatc
     9421 aatccatctt tcattaaact aattatacaa gcttatctta ctcctggcta gtgaagggca
     9481 acagagccaa atgtcgggat tgctttatag caactgccaa ctgacgctga tgctttacac
     9541 agacaccggt aaaagaagac ttaagtatac ggcttcgagg tgacaagtat tcagccaatc
     9601 tttcgacatc actccatact ggctcaacat tagccttaca aaaagcacat tcccgagaac
     9661 ggggtctcat tttttctttt tttggtctta ttcccatatt ttccttttaa ttatttatta
     9721 tcgtcaacta atggtatttc tccgatttcg tcgggaataa caatgtcttc aatctcacca
     9781 ttgggtgtaa tctcgacttc ttcagccatt ccaccagcaa ccggttgatc aaaatgacca
     9841 ccttggttac caccggacat agctacacct ttgttatcta agataatcat gtcatcaatt
     9901 actatttctg acacttgttt cttgacgcca gtagagtcaa cgatctcacg atattgaatt
     9961 ctaccttcaa cataaattct tctacccttt gataaaagtt gtgaacataa ttcagctaat
    10021 ttattccaag ccacaattcg atgaaattga gcatcttctt ttttctcacc ctgtttggtt
    10081 atccatactc tattggtagc caagccaaag cttgatactg ccattccaga tggagtataa
    10141 cgcaattctg gatcacgggt aaggttccct attaacagga ctctatttag acatctactc
    10201 ggcatatgtt tctactttaa taaatattta cttacctttt acaggtttaa tattaacggg
    10261 attttttaaa atcaaatagc gaatgacatt tgattctcta tttaagaaca agttaagctc
    10321 ttgcaatttt actggcttgg gtgattcgac cataaaagac caaaagtcac cttttctcag
    10381 tttagcaatc tcgtaaacaa gttctttgac acccaagttg tctttcttga ctatcttagc
    10441 ctgactctcg tccaaccaag cctcaacttt ggcaaaaact ttttctttct ctacttcttc
    10501 cacttttggt gcaagaacca gaaccatttg atatttgtgt tgattatctt tcataaaact
    10561 atctagttta tcatatcttc cacgactttt gcgggatcaa aatcagcgtc tacaacttta
    10621 agtatttgat taataagttc ttcgggtatt tcttcatttt ccccgtactt ttccaacatt
    10681 gcttcagctt tcaacattgt atcagccgaa acctccatat caactattat ttttcgtgca
    10741 tccgcagctg tcattagatt tttttaaaat tataaccacc ttctttgtcc tctattatat
    10801 aacctttttc tcgaatttca tctcttaact tgtccgcttc tgcccagttc ttagccatct
    10861 ttgcttcaaa cctctgtttg cctaaggctt ctacctcttc gggtattatc tctttaacaa
    10921 tttctctacc caggtccaac cccaaaattt tatcaagttc aagaagtgtt gctttaatat
    10981 cccgaggctc gtagtcccct tttaccattt tccaaactac tgccatcgcc tccggcatcg
    11041 acagatcatc accaacttta ttgacaaaca tctccaccac ttcgcttaat acctttcctt
    11101 ctccttcgac ctgagctgct tttatcaact tatttaatga tgtctgagcc acatccaata
    11161 gttgaaaggt aaaatccatc cctttcccat aactagatgt caaaatcaga tatcgcattg
    11221 ctaatggatc ataattcttc tccaccaaat cagacactaa tacaatgttg cccaagctct
    11281 ttgacatttt cgcaccttcg tatgtcaacc aggcattatg tatccaataa ttagccgtct
    11341 gatgtccaaa tgctccatag ccttgagcaa tttcgttagt gtgatgtact ccgatatgtt
    11401 caatccctcc agtatggata tcaaatgtct cacctaagta cttagtagac atggctgtac
    11461 attcaatatg ccagccaggg aaaccttcgc cccaaggact cagccacctc atgatatgtt
    11521 ccggttgatt aattacccac aaaagaaaat cccaaggttt tttcttttct tcgtccactt
    11581 caactctatt ccccgagtcc atctcttcaa gtttcaagtt ggcaaactta gcataatcag
    11641 caaatttccc agtatcaaaa actaaccctg tttttgttag ataagcaaaa ccattctttt
    11701 cgattttccc agccaactca atctgttctt ctacatgctc agttgctcgg caaatagtat
    11761 taattccctt aatattcagt agtttcaaac tttctctaaa ctgctcttca tattttttgg
    11821 ctacttccca cacagtcaag ccttctcttt ttgccccttt ttccatctta tcgtcaccag
    11881 tatccgcatc agatgtcaaa tgtcccacat cagttacgtt cattacccaa ttcacatcat
    11941 tacctatata tcttaaaaat cgcaccaaag tgtcccactg aacatagcaa tacatgtggc
    12001 caatgtgctg gttccaataa actgtcggac cgcaggaata tattcccacc ttcccctctt
    12061 caattggttt aaattcttct ttctttcttg tcgctgtgtt gtaaaaaaat actttttgca
    12121 tagttaaatt atatccaatt attaactaaa cagtttagta ctaatatatc tatctcctcg
    12181 atcaggaaaa atagttacaa ttgttcctgt tttaatagtc ctactaacct ctacagctcc
    12241 ccataatgcc gctccagaag aaattcctac tgacaaacca tcgttcttaa acaactttct
    12301 tgccaagtca aaagcctcgt catcaactaa tgttttaacc tcatctatta attctcgcct
    12361 aaaaatcggc ggcatatagt tctctaaatt tctcaaacct tgtatttctg aatttttaat
    12421 cggctctagt ccagtcacct taatattttt actccactct tttagccttc tactcacacc
    12481 cacaattgtc cccgttgttc caattcctac taccaaatta gttacatcag ggacgtcttt
    12541 aataacttct aacccagttc cgaagtaatg tgccaacaca ttattctcat tttgatattg
    12601 atccaacatc agatatttat ctgattcttt cctgaccatc tctttagcca tatcgattgt
    12661 agttgtctta ctagtatcga ccaaaatcac cgacgccccg tatgcctcaa gtaatttaat
    12721 tctctcaata cttgcatgtc gtgacattac cgctacaaac ttataaccaa acacaggtgc
    12781 cagcatcgcc aaaccaatcc cagtgttgcc gctagtcggc tctaccagag tcaacccatc
    12841 tcttagcaac cccctcttac ttgcctcact agccattctc caggccaccc tatcttttat
    12901 cgatccccct ggattattac cctctaattt ggcaaaaatt ttaacctttg aattcaccga
    12961 atacttcgac agatcaatca tcggcgtttt tcctattaac ttgtttaatg taaatttcat
    13021 aatattttta aaaattttta aataaaaaaa cctcccaaat ttgggaggcg tacgatacgt
    13081 taaaagcctc ccctaacagg aggtacaaca gatcattaca aaatacttag acacaggctt
    13141 cattacgcta tattttatca ctaattttca tttttccaaa aaacttcatt ttcaacaact
    13201 tcttatccat agtcaaaaag tagtcacaat ccccttcagc tgctgagttt aactgtacat
    13261 tatcttcaaa atcatctgtt ggtcccataa tcgatttttt aactatttca ttagttaact
    13321 caacagcagc caacacctca gacattcttt ttagcttttc atgaggcatc ttccatttca
    13381 tcaaataaca agtaatgtgc caggacaata ctgaaatcat caaattatga ccactcaaat
    13441 tatcgatctc ttcaccctta attaagccaa ttaatctatt tacgtccaga aatatatttg
    13501 ccatgctttt tctccattgc aactctataa gccatatcca tttcttcaac ggtcatcgat
    13561 tcttgtttag gaaaactttt tactaaatca agaaaagtac tttttgtttt ttttaccacc
    13621 ttctttggcc caatctctgc taccacttct ccacgaagca ttattcttgt aatttgttta
    13681 ttcactctag ctgcctcaac tacatcaaaa atgttttttc ttaattgagt taccgacatt
    13741 gtcttggtca tgcattaagt gtacatcggt gtacatattg tgtcaataga ttactttatt
    13801 ccatcttccc acccttctca gatgtctgag acatcgtggt tggatctggt tgctgagtct
    13861 ttctcttgcc ctttacaaac gcactccctc gagatttctt ttgtttgttc ggattactcg
    13921 acaaacccat ttgcgattct agttgcatcc tctctctttc ttctgcttct ctttgtgctt
    13981 taatttgttg taacatttgc ttttcctgct catcttcctc ctgttctttt tctttaacaa
    14041 tttgctttat ctctccctcc acatttcgtc ctgagatctg agatctaaga tctgagatct
    14101 ctttctggtc ttcagcctgt tttttagagt attcttcacc actcaatgtt tttacatctc
    14161 ccaataactc ttttcccgta attatcgact cagttatttt tccagtttcc aaaacggact
    14221 cttttccagt ttcaactact atctgcccca aatttttcaa aacttttttt agtattgaat
    14281 cacccatatt gttgattaaa ttgtccttct tccctccata gctctactca aagttgcctg
    14341 atcagcaaac tccaaatctg cccccattgg taaaccacta ccaattctag taattttaag
    14401 ttttaagttt tgagtttgaa tttttttatt tatatacaat gctgttgcct ccccttccat
    14461 tgttgggtta gtcgccaaga taacttctga cacctctcta ttttctatct tatcaatcag
    14521 ttcattaatt ttaagatctt ccggccctac atgattaagt ggattaactg ttcctcccaa
    14581 cacgtggtaa acccctttgt atccacctaa gttttcaatg gcaatcacat caatggctcg
    14641 ctccaccaca caaatcaaac tcttgtctcg agaactatca ctacagattt cacacacctc
    14701 actttcactg actccaaaac attcattaca gatcttaata ctttttttca tttctaaaag
    14761 gtcattacta atctcctcca caaaatgttt tggagcattt agcaaataaa atgctaatct
    14821 agtagcactt tttggcccaa tacccggtaa tctttcaaag acaccaatca aatcaacaat
    14881 aggtctaggc agtgcagtca ttggttacat ggcaccttct aagtctttac ccatctcttg
    14941 catctttttc atagaagact cagtggcctt ctttaacgct ttatttatga cttccaagag
    15001 cattctattt tccactccgt tcaccactaa tttctttatt tttggctcac tcatcgccat
    15061 aaatcctcca acatctatct cgattccact ttcatccaaa gggtacgaca catcttcaat
    15121 cgcctttttc attttaaaca cctgtattgc cattttagct ttgtcaaaca tattttagaa
    15181 aaaacttttt aactaccaat tctatcatct aatcccaaac tttttgcggc gtcctaatat
    15241 ctcttgtcta atttttaata ctttttgcgg attatccata atcctctcaa attgatccga
    15301 gtccttttca tccatttctg atacctgacc acaaaaagcc tgattatctt cttttctacc
    15361 aaacaactca gtacgaacca ggtgaaaagc agcatgctca ggccgactaa gacctaacat
    15421 cctttgctga gctgttactc gccttctggc cactggcatt tcaaactgag ccggttcatg
    15481 gtgagttgtt tgtgccgcat catccccaaa aaatcccatt cctcttcgtt ttggaccttt
    15541 ttgataggca tcactctttt tatattcttt ttgcgaaatt ctaacttctc gccccttcat
    15601 ccaaatatct ccttcgctac gtcgtaaata tcatcgctag acgcgtcttt agccacatct
    15661 gctttttcat tttctaccac cgggctactt tggactataa gatctccacc cctatcgtta
    15721 ttcttgccca aaacacactc caaggtcacc ggagaaccca caatctggct caagccattc
    15781 tcaactatct gtcgattctt ttgctcttca agcttatctt tatgaaatgg ataataaact
    15841 tccaatgtta ttacattacc atctatagat tttggcctag ctgctcgaag aaacgcttca
    15901 accgaatgat taaacggctt aactgctgtc aatactttcc cccaatctct cattatttcc
    15961 tccacctcca ccccaatatt cacactactc ttcgcctcat tctttatttc tattttttct
    16021 attttctttt cttcaggctc ttctttagac tttagacttt tgactttaga cttttgactt
    16081 aagaactctg ctactgccaa ttgtaacggc aattgttcta atcccacatc tttctcttgt
    16141 ttacccgcca caattaatag ctgcaaccac ttttccaggt ctactacagc aatttttgac
    16201 acacctcttc caggcacacc caaccctcct aataatttgt cttgaaaata aaccaacatt
    16261 ccctctcgat aagatctaaa atctgcccct ttttcagcca ttttctcaag tttttctaat
    16321 attacagcca cttcaccaac caccatatct tcttcaattt cttcgtaaga atacccacca
    16381 ttcttctgat caaaataact ctccacattc tccaaagata tatcctgtcc ataacgcaaa
    16441 actatctcat taaaagtttt ttgtaaatta cgaaaacttc catcactctt agccaccacc
    16501 atttcaatag aagcctcatc aatttttgtt ttctcccctt ccaccactct tctcaaagat
    16561 tttcttaact cttctggcgt tcctttccta aaactaacct taatcaacct cgacattatt
    16621 gtatccggta tcttttcttc atcagtagta caaagcacaa aaatagtatg ggccggtggc
    16681 tcttcaatca atttcaacaa tgcattaaaa gcatcccgag tcatcatgtg tacctcgtct
    16741 ataatgaaaa cctttttctt taatttgctt ggcaacagat atgctttctc ttttaaactt
    16801 ctggcttcat caattcccct atttgaagcc gcatccattt caattacatc aagacttcca
    16861 ccttttacta tctctttgca attatcacac tctccacaag gatcaccatt ttccggatta
    16921 aggcaattaa ctgctctagc caatattctt gctgccgaag ttttacctgc accctttggc
    16981 cccacaaaca aaaacgactg gggaacatcc ccactattta agacttttga taaagacagc
    17041 ctaacctcag ccaaatccag atccgctatc ttttgtggtc tgtattttaa gtgaaacact
    17101 gtcatgcaaa ccattctaga tgaattaccc tctaccagca atgagtctaa ctatatcaac
    17161 aaattctcaa ctccatgaac catccattct tccaatactt tttcaattgt gctattttga
    17221 aactcaactt catatttcaa tttctgtgta caaattacta tatcccctaa atgtatcaaa
    17281 ccatcacttg agatctcttt gtccatggga aagcccagta cttgaggtat ataggtttta
    17341 ttacgataag tcacattcaa gtctttagct ttttttctac ctacaaaaat tacactcaac
    17401 tccaccccat ccaagccctt tatcttcaac gctttttttg cctttttaag cactaagtcc
    17461 tcatcaatac cccaattctt cggatgtttt atcaaaatta catttgacat ttttgtttta
    17521 cgagaggcgc aattttttct ccacctactc tgccagatac cttggtcact acaattctca
    17581 tcacatttcc aaaaacttta ttcccttcaa ttattgcctc atcaacaatc ttttccaatt
    17641 cttcatctgt catctctgct ggcaaatatt cttttaaaac ggatatctca taatccaact
    17701 gttttaccag atccattcga tttcccttaa caaacatctc tctcgactcc tccttattct
    17761 ttagttcttt tcttaataca gacatctcct cagcctcagt catctgtcca ggtggtaatt
    17821 gtagttcttt tttatcaatt aaggatacta agaacctcaa tacatccacc cggacttgat
    17881 catgaccttt caaagctgat actgcatcgg cctttaattt atttttcatt taataccttc
    17941 tttttttggc tcttcgacga catttttctc gccataagat tattttcttt ttggcataac
    18001 gcgccttagc aggcttaaca taagctgttc tttttcggac ttcttcagtt atatcctcct
    18061 caatagaaag cctacgaaac ttacctatta catcgtcttt tgattcacct tttttctttt
    18121 ttacaataat tggcatcaat ccacctcctt tttaattctc gtattctacc ttattcttat
    18181 ttcttttgta aacacttaat tctgtcaaca gaattaatta ctccattacc atcataatcg
    18241 acacct
//
LOCUS       DDWP01000029            3782 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_34200, whole genome shotgun sequence.
ACCESSION   DDWP01000029 DDWP01000000
VERSION     DDWP01000029.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_34200"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1063 a          808 c          617 g         1294 t
ORIGIN      
        1 agcattatgt acatgtttat cgccgacaac atcattcccc caattaagaa tgcattccat
       61 ccagaattaa acggtagtat taccattaga atatttagtg gatacagtgc tcctgaatgt
      121 aaattgccca gtagggggta tccagaatac gaatattgat tccacagtgg ccattcacca
      181 attttatagg catcggatac aagttttttc cacacaaaaa attgtgagta agagtctgat
      241 attaacggat tcttataagg taccgccacg tcgtagcccc agttattttc tctccatgga
      301 tagtacgccc ccaccaataa gtcagttggt actagtactg ccccctgcgc tattactttc
      361 caaaagaacc ccccgattat aattattatt aaaaatatgg gccagagttt tttgagcaca
      421 agtccatttt acattctttt gctctataaa agacgagccg attcttcatc taagacaata
      481 gttactttct ctggttgtgt tcttaatata gtcgacggtc tttctactcc aattttgcct
      541 ttaagtgtct ggtaaattgg tgcagcatat ttctccccaa aagcatttaa aattattttt
      601 tttgattcca ttatgtttgc cagtccctga gttaacgctt tgtctggtga atcctgacct
      661 ctctcttgcc tatctctagc cacggtaaca gggtcaagtt ctgttacgtt aactcggctt
      721 tcaaaaggtg caccagactc attgaaagct atatggcagc ctcgttcatt ttcggcagac
      781 catggaccga ttcccagtat tgttaagtct ctcggatggc tatttagtaa atcttcatat
      841 cgctttgctt cagcttcaga gctttgagcc agtccattca gttcaaactt gttggctata
      901 tttaatggtt caaacactct ctggcgtaaa taatttacaa acccgtatgg ataatctcct
      961 ccagtaccac aaggataata ttcatccagg tgaaaagcgg tcgtctctga gaaatccact
     1021 ccactcttta ctagcttttc gtagactagt atctgactat ttcccgtggc ataagtaatc
     1081 actgctttgg gattatcttt taccgtttgg gtgactatgt cagcagatat tgttgttgct
     1141 tcttccaaat ccttaccaat cagcaccgct attttatcga ttgttatttg tctcattatt
     1201 tcattatact ccttgacaaa aatgttagtt agatctaaca tatgtagtta gaaatattta
     1261 acatgaaaac tttaatcaaa gaatatcgtc aaaagcttga tctcacccaa gacgagctag
     1321 ctaagctagt caatgttcgc cgtgaaacca tcgttttttt ggaagccggt aaatacaacc
     1381 cttctctcaa gttagcctac gatgttgcta aagttctcaa agttaaaatt gataaattat
     1441 ttattttcta gattatgatt gcaaaatctt cttggttctc tattagaaag tatggcggtt
     1501 ggggattgac cccaaactgt cctcaaggtt ggctttatat tattatcgcc attattcctg
     1561 ttattttaat taaaaataat tactttgttg ccatttggtc tttattattg gctcttgatc
     1621 ttatccatgt ctttttcaat ctcaaaaaag atgagaggga gactcttcac gaagccattt
     1681 ccgaccgtaa tgcactatgg tttatgattc ttgctttagt tattggtagt ttaatcaaac
     1741 aaactatcga tcctattatt ctggtcgctt tactcggtgc tagtttggtt aaatctatca
     1801 ctagttttta tcttcgcgat aaataagcta gaatagttca tgtctactat ctatcttttc
     1861 cgccatggcc aaaccgattt taatctacaa aaaatattca cagggtggct tcagtcagaa
     1921 ttaactgata ttggtatcca acaagccaat aatatggccg cgctcttaaa agaaaagaca
     1981 ataaatttag cttacacccc agaccttact cgttgtcaaa acactctcgc tgaagttttg
     2041 aaattccatc cagaatgcca agaaacaatc attgacaacc gcattcttga aagaaattat
     2101 ggcgacattg gtggtaaatt acatcaaaac attatagacc agtttggtga agaacagtac
     2161 aagaaatggc atcgcggctg gtccgaccgt ccacctggtg gtgaaagtta tgccgatgtt
     2221 gaaattagaa tccaagattt tattactgat ctccgctcaa aatactccga taaagattta
     2281 agtattgcca tttgtggtag tagcaattca attcgtcttt ttcgtaaaat catggaaaat
     2341 gccagtatcg aagatactgt ttcctggact attccttacg atcagtattt tgaataccaa
     2401 atataaggat tatttcttta ttttttttag taattttttt atttctgcct ccatttcttc
     2461 aactgttccg gatgaatgat aaggtgcgac tctttttttt ccttcaaaca ccaccaatct
     2521 atctgtccag acaattctcg gctcttgtct tggtggaatt ttaccgcgcg taattcttgc
     2581 atcttccttg agaatctctc ccaatggtaa agacgatgtg tttaccccca aaaacatttc
     2641 tggccttcct agtacatcat gatttagttc gtacaccaaa cgacgaccgc ctatctctat
     2701 gccctgttct tttcctctcg ccacaatctc gtcagccact ctaacaaacg ggacgacctt
     2761 ttctcttttg gaaatttcct cttttaatat atcggtttct aattgattga cttggccagt
     2821 tgacggctcg gctcgttttc gaatttcata aaaatatcca actcgacgac cgttattttt
     2881 ttcttgtttt ataggcatgg gcgatgatat tctgaattgg ctagactttc aaaccagttt
     2941 ttaagccata caaaaacaca tttagcttta ttttttagtt aaaaatgcat aaaaacattc
     3001 acttttatac tttgccatta actttcttcg tgctatcatt tccccgctat gaaaaaatac
     3061 aattttccca cacttactga catcctcact gtcaaagttg gtacaaagta tgtctgggaa
     3121 gatcgtgtcc gtcaacagtc tactctagtt catctccttg aagacgtcta tgatattaag
     3181 ttaaaccttc gcgcattttc tttttatccc aaccaacttt atttcgggat gccacttact
     3241 ttcaactcca ccttaaatac cgacaaaata ttatggaacg acattggtaa aatttttcaa
     3301 aataatcatt ggaaaacgta cttcgcttat gaaaacgtca atccagatct tgatattcca
     3361 gctaaaatcg acacttttga tgatcttggt ttcggtcatg ttcaacttct tttgtctgaa
     3421 ttagtcgtta tggatttgaa ccatccctcg tttggagttg gtcaacaact agaattgtct
     3481 ttgtttcaac ccttgatagg tttctctaag agcccgccat ctcgcatggt caaaggtcgt
     3541 cctggaagta tgattattaa gtacgatacc gaagatgaac tgcgaaatat ttttcaagag
     3601 atctctcgtc gcaaaagcta ttcacgagag ccattttatg tcaaaaaaca cccatctcgc
     3661 ccattgaaat ctgtttacaa aggcaaggtc tgtctcaacg aactcttcaa gaacaattgc
     3721 tattgattta gattttcctc aattctcatc agacgattat atttagagac tctttctcct
     3781 ct
//
LOCUS       DDWP01000030            3735 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_29095, whole genome shotgun sequence.
ACCESSION   DDWP01000030 DDWP01000000
VERSION     DDWP01000030.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_29095"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1242 a          762 c          646 g         1085 t
ORIGIN      
        1 cggccaaccc gtcaaagatc atttacatga aaagaaaaaa caaccagtag atctacaaaa
       61 cgtagataat ttaccaccag atagttcaaa aagatttatt gctgtcctca acagtaaagt
      121 tgatatgggt aaattagcca acgctcttgg tcatatgact gcaggtctga cttcaaattt
      181 tactaaagaa gaattattgt ttttacaata tcaagaccaa gatggaacac aacatcctaa
      241 tatctcccac ttcccattta ttgtcttaaa agcatctaac tcaaatcaaa tcagaagtat
      301 ccgtaatgaa gctattaaaa aagggataaa gttctctgat ttcactgaca caatgacaat
      361 aggaacatct aaagaacagt tagaagccac tataaatact agtgaaaaag acttagagta
      421 tcttggtata tgtctctttg gtgacacgac agtattgaaa gaattcactg gtaaatattc
      481 attattcaaa taatataaat atgaatcgtc aacaatttca tgatgacgat cttcaagtcc
      541 aaatattaaa acctccattc tttgacccca ccggagttgt tattcctcga acagaagctt
      601 ggagtaaagg tctttggtac ggtgcagcca atctttgggt agttcaatca gatccagtcc
      661 cttcaattat ttaccaacaa cgtagcccta atatcggttg ggcaccaagt aaactagaca
      721 ttctaattgc tggtcattgt gaatttctac acactcctct tgagactctt gcacaagaag
      781 ccttagagga gatgagcgtc gagtatgacc aatcacgatt tatttcaatc ggtaaaaaat
      841 tagccgtcaa tatcggacaa gacggaacta ttcgcaattc cgttaattat atttatctag
      901 tcgaagacaa cctacctatt gaccgtttta tcatgcaaaa gaaagaggtt tacgccattt
      961 gctcatgccc acttagtgaa attctcaaag tccacaccga ccctaattat tcttataccc
     1021 aaccagccat caagcatgat ggcagtaatt ttgatttaac tatcaatcaa gacatctttc
     1081 ctcccaattg ggatccatac cattacaaga tggccttatt aatcgatagt tatcttaaag
     1141 gtaataaaaa tcttatatat taataactaa ctatggatcg acaaaaattc tatgatgcag
     1201 ccaaagaccg attagaaact aacatttttt atcattccct cgctcttgaa gcttgtatgc
     1261 ttggacttta tgactatttt tcagccaaca acttattgga caataatgaa ccaactcgag
     1321 aagagtgggg gattgctggt ctaattcacg atatcgattt ttctggtgat actaaagaaa
     1381 atcaccctct caaaacccga gaagtattat ccaaatacaa tctcgaaatt aacgacacca
     1441 ttcaacaaat aattttgtca cacgacaaga ggcagggaac tgccatggat agtaaagcca
     1501 aatggtcaat ctattgcgct gactctctca ccggtcttat tatggccgtt gcctttgtct
     1561 atccagatcg aaagctcgcc tctgttaaag tctcttctgt taccaaaaga tttcttaaag
     1621 aaccaaaatt tgctgccggt acccgtcgtg acgaagtggc agaatgtagt cttcccgaag
     1681 gcttaaattt acccttagaa aaatttatcg agatctgcct cacttcaatg cagggaatct
     1741 cctctacact ggggttataa ccagccccga caaaattggt tagccaaatt actgtaattt
     1801 tacggtaata aaataattaa tttttgttac aataaaggag atccatgtta gacataaatt
     1861 taatccgaaa cagtcctgat acggtcaaaa ctgccctaat aaaccgccaa aaagatactt
     1921 ctgtcctaga tcaagtaatc ttgctcgaca aaaattatcg agaaattctc agtgaagtcg
     1981 aaggacttag agcttcccaa aacgccatca ccaaaaacat taaaggtaaa cctagcgacg
     2041 agcagatagc tgccagtaaa gttattaaag accagctcaa agacgttgaa gaaaaattaa
     2101 aaaattctga agtcgaacta aatgctgtcc ttgaagaaat tcccaatatc ccttccgacg
     2161 acactcctat tggtaaagac gacaatgaca atgtcgttat caagactttt ggtgacaaac
     2221 ctgccttcag ctttactcca cttgatcaca ttgatcttgg cgaaaaacta gacattcttg
     2281 atgtcaaaaa agctgctgaa atatctggtt ctcgttttgg ctacttcaaa aaccaaggtg
     2341 ccgtactcga aatggccgtt atgttttatg cttttaacaa actggttgct aaaaatttta
     2401 aaggtatggt tcccccagcc atgattaaaa gtcagacaga atggggttgt ggctatgcca
     2461 gcaataaaaa tctaatgggt gcctactatt ccatccctga agacgactta atttttatca
     2521 gcagttcaga acattcagtt gttccctatc actcaaacga agttttatct gacaaacaat
     2581 ttcctataaa atacgtcaac tattctccat gctttcgtcg cgaatcaggt acctatggta
     2641 aagatgctaa aggtctattt cgtgttcatt ttttcaacaa agtagaaatg aatatcttta
     2701 ccctaccaga ttttgaaatt tctgatgcca tgtgtcttga aatgttatct attgaagaag
     2761 agctactcca agattttggc cttcactacc aagttgttaa agcttgtact ggtgatttac
     2821 ctcagccaaa tcaccgaatg tacgacacta atacttggtt cccgggccaa gatcgttatc
     2881 gtgagactca ttcatgcagc aactgtggtg attatcaatc acgcagatta aatacaaaag
     2941 tcaagataaa cggagaaaac aaattcgttc acatacttaa tgctaccgtt attactgatc
     3001 gtgccatctt ggcgattatt gaaaatcacc aacagcaaga tggcagtatc aaaattcccg
     3061 aatgtctttg gccactaact ggctttaaag aaattcgccc taactagcag aggagccacc
     3121 caacccatcc cgcctcggcg gggtggggta ggtctttttt ttatgataaa ttatcaatat
     3181 tgtccccagt gtaaaaatga tctagatctc tctcaggagt ttccttattg ttctaaatgt
     3241 gatcttacta tatataaaaa ttccaaacct tgtgtctcca tcttgcctat taaagatggt
     3301 aaggtgctct ttgctaaacg tggtattgaa ccttataaag gtgattggga cactatcgga
     3361 ggtttcttaa aagctggcga acatcctgaa gtgggtgcct tacgtgagac acttgaagaa
     3421 actggtttaa tagtcaaact tacgggacta ttgggtatat atatggacaa atacggccaa
     3481 gacggcgaag atgttcaaag taatgtttac ctagctgaag ttatctccgg agaaataaaa
     3541 ccacaagacg atgtcgctgc tttagagtgg atagacataa aaaatggcaa aataaccagt
     3601 cattttgaaa gcatagaaca gatttttgct gatctaaaaa agctatattc atagtagaat
     3661 ggtctatgtt tgatttcata aaaaatatta tcgacgataa tcagcgacgg attagctcct
     3721 ataaatctat catta
//
LOCUS       DDWP01000031            9078 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_30229, whole genome shotgun sequence.
ACCESSION   DDWP01000031 DDWP01000000
VERSION     DDWP01000031.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_30229"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    8772..8839
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2663 a         1828 c         1873 g         2646 t
ORIGIN      
        1 tcccctttta ggttggaagt tagataaaca gtcttacctg aagaaaggat gagggtatat
       61 tcattttgat taagtggatg aatggcaaaa tattcttggc taccacctag attagaggta
      121 ataaggttag agatagactg ggaaacaggg gtaaagttgt atgacttgag atgaggaagt
      181 gcttttagat tggtctctaa agcagatatt ttgttacgct catatatatg ggcgagaata
      241 atggttttgc catcagatgg gataagttga tagtcaatat tatcgggatg aatcttgaaa
      301 ttaatgaaac tttctgcaag accaataacc tccgccttat ctattgtaga gataactgag
      361 tcgtaaataa aagacttagt gaagacgaca tcgtcgggca aaagaatagt gcaggaatca
      421 atactatttt gttttaaaag cagatcaagt gactgtagtt gagctggagt acaagcttgc
      481 cacagattaa aatcaagaga aaagatatta ctgttcgact tgtctagata aatgtcaaca
      541 gaattatttt taggccagat gactgaagta ggtttggaga acattgacat tagataatgt
      601 tagcacacta ccagcgcaaa tgagcgaagg ctttgttggc atcggccata cgctcgactt
      661 cgagacgctt gttgactgat gaaccttcgt tcttgagagc agataatagc tcgctcgcta
      721 gtttttgacc aaaggagtga tattgtttgc tggatagagc tcgggtgctg gtgattaacc
      781 aacgaatagc taaagacatc tgtcgacgac cgtggactgg ggtaggaact tgatatacag
      841 caccaccaat gcgacgaggt ctaacctcaa cttttggttt gatgctatca agagcctgct
      901 cgagagtgac gagaatatca tccgacttag tctctctctg aacgatatca atagcttcgt
      961 agactccttg agtagcagca ctctttctac catccatcat gcagtaatta atgatcctag
     1021 caatagtatc actgttgtag cgagtatcgg gaacgatctc tcttggttta acctgacctc
     1081 ttcttgacat agtttagttt gtaaagttta taaaagttat aaattattct gcagatttag
     1141 tcgggcgctt agcaccgtat ttactgcgtg attgaagacg accggcaaca gcaccagcgt
     1201 catactttcc acgaacaacg tgataacgaa tacctggcaa atctcggaca cggccgcctc
     1261 gaatggagac gataccgtgt tcagccaagt tgtggccgat accgggaatg taggcagtaa
     1321 ccgtgctatg gttgcttaat tttattctag caactttacg caaagcggaa tgtggctttt
     1381 ttggagtcat ggtcttgacg acagtaacaa cccccttttt gaaagggttg aacgtcttaa
     1441 ttgttcgatt cttaatgctg ttaaaattgt cgcgtaaagc agcagctttt aactttaaag
     1501 tacgaacttt tcgacctgat ttgacaagtt ggttaaatgt tggcattgtt tatttttttg
     1561 aattggctaa aacctatttt cgactaacag gtattagacg tccgataata acattttctt
     1621 tgagaccaat caagccatcg acttcagcaa gtaaggatgc ttcggtcaaa acagaagtgg
     1681 tttgctggaa ggaagcggct gatagccatg atccagtaga aagagcggat tggataagac
     1741 cgagtaaaat cttcttaccg acacttggtt taccgttatt cgctttagct ttttcattgg
     1801 cctcaagata aactgcgcga gtaattattt gaccatacag gaagttagta tctcctggat
     1861 cttcaatctt gatcttgtcg ctcatttctt taaccatgac ctcgacgtgt ttgtcgtgga
     1921 ctgatatacc ctgagactca tagacagctt ggatactatc gatgagatat ttctgagcag
     1981 cctcgatacc cttaacttcc attatctcac gaacatcaag tgagcctgag caaagttggg
     2041 caccttgggc caccaaatcg ccatcagaaa cgagaagggt tacattgctg ggaagaagat
     2101 aagtaacggt cttagtgact ttcttatcat ctttaccagt aagttttatt tcgtagccat
     2161 caagactttc tttgactttt accttaccgc ttatttcagc tagaggagaa ggaacttttg
     2221 gcattctgac ctcgaataat tcctgaatac ggggtaaacc ttgagtaacg tcgacaccga
     2281 cgacacctcc agtatgctta gttcggaggg tcaactgtgt accaggctca ccgatagact
     2341 gagcagcaac aacaccgaca ggaacaccaa tcttaaccat ctttcttgta ctaagatccc
     2401 atccataaca tttgttacag ataccaacaa tagctttaca agtcaaagga gaccgaatgt
     2461 cgacggattc aacgtcgcta ttttctataa cagctacctg ggcattacta agaaggtcac
     2521 ctttactgaa aagtaccttc ttgttttttg gatcaataac atcgttggct agaactctgc
     2581 ctaaaatgcg tcgaccaaaa taacgttcac gattcttgtc agtcttacta atagtaatga
     2641 acttcagagt attacaatct tcctcacgag tgatacagac atgggcggca tcaactaaac
     2701 gtcgagttaa gtaaccggcg tcagcagtac gtaaagcagt gtcagcaaga ccctttctgg
     2761 tacctctggc accagtgatg tattcaaaga cggaaagacc ttcacggaag ttgctcttag
     2821 ttggcaatgg gacgatatga cccataggat caaccatcaa tccgcgcata cctgaaagct
     2881 gcttgatctg atcttttgag gcacgcttga taccagcggc ggccacgatt ttgataggtg
     2941 aatcagaacc aagggtactc caagttttgt cagcgatatc ctcggtgatt tccatccaaa
     3001 ttgattggct aagtctcttt ttctcatcat tgctgataag acccatcttg tagttgtctt
     3061 caatttcacc aacacgtttg ttggcagcct gaataatggc ttccttacca ggtaagtatc
     3121 cacaatcagc catagataag gaaattcctg atagagtgaa accagcaaaa ccaatatctt
     3181 tgagatcgtc aatcaatcta acagctcgca tacggccgac ttcttccata gtgcgaatca
     3241 ccaagtcgga aacagatttt ttaccagcca taggctcgtt gagaaatcca aactccttgg
     3301 ggacaatagt gttgaaccaa attcgaccat aagttgtttt aagcaacttg ccatcgatac
     3361 gaacaacgat taactcacgc aaactaatct tttttgattg ataagctaag gtcacttcct
     3421 cggcatcaga aaatattgaa agtccttcaa aaccttcttt tgattcagac tctaaatcag
     3481 agttttgaac agaagttacg taataacaac cgacagccat ttcttggccg gggatggaga
     3541 taggactacc atcacttggt tttaacaagt tgtgagatgg caacatcaag gtcttggcct
     3601 cacgaataga agcttttgac aatggtaaat gaacacccat ttgatctcca tcaaagtcag
     3661 cattgaaacc ggcacacata caagggtgaa gtctaatagc caaaccatcg gttaaaatag
     3721 gtttgaaagc ttgaatagag agtttgtgta gagttggagc gcggttgaga agaacaaaac
     3781 ttttcttagt gattctctca agaatgtcat aaacttcact aactcgatga tcaattaaat
     3841 tcttagcact cttaatgttg gcagctaaac cactgacaat tagttctcta agaacaaaag
     3901 gacgatatat ctccagagca atttctttag gaagacccac ttgattaagc ttaagatctg
     3961 ggccaaccac aattacggaa cgaccagaat agtctacgcg cttacccaat aggttccttc
     4021 ggaaacgacc tttcttacct cgcagtagat cagatagtgc acgaggagtt ctcttggttg
     4081 atttacgctt attcttggaa ctcttctgag catcaattaa ggtgtctaca gattcttgaa
     4141 gcattcgctt ctcatttctg agaatgattt ctggtgcacc aagttttaag agtttcttaa
     4201 gacgattgtt acgattaatc aaacgacgat atagatcatt taagtctgaa gtagcaaatc
     4261 tgcctccagt gagctgaacc ataggtcgaa gatctggtgg gataacggga acgtgagtaa
     4321 gcaccatcca atttggatta atactgttct caaccatacc gttgataaca cggatcttat
     4381 acatgatttt cttctttttt atcttgctac tagtcttgga aagttctttc ttgaggttct
     4441 taacggtctc tggtaaatct agattaccta agacttcgta aatagactct gcacccatct
     4501 tagcttcata gaaaatatct gctttgaact gtgaaaggta aaaactttct tcatcactca
     4561 aagtagagag gacttcgatt gattttatct tgtcttctaa aaatttaata agactctcga
     4621 tacgattctt ttctgataaa gctttgtttt ctagcttttg gacatcttgc ttgaggcgga
     4681 catcacgttc agcagtagca ataaggagtt ggtctttatt cttgatttta gtttttaaaa
     4741 cgtctttttc atcgctagta agcttggtct tgtcggtaac agtctttttg agatcaacat
     4801 caatctgttg gatacgttcc ttggcattgg tattgaggtt ggccaaagct tcttttctct
     4861 tatcatcatc aattgaggta ataagatatt tggtgaagta aacaactgac tctagctctt
     4921 tttggggaac gtcaagaatg acactgagag gtgagggggt gttgcgaaga taccaaagat
     4981 gagtaaccgg ggctgcgagt gagatgtgac ccatacgctc acgacgaact ttggaagtgg
     5041 taacttcaac accacacttg tcgcagataa tgcctttgaa acgaatcttc ttgtattttc
     5101 cgcaataaca ttcatagtca tgactaggcc caaagatttt ttcgcagaaa agaccatctt
     5161 tctcaggtct ccagctacga taattaattg tttctggctt aagaacttca ccgtgtgacc
     5221 aagaaattat ctcatcaggg gaagccagct taatctgtaa gccggtaaaa tcggtcatgc
     5281 ttttaaattt aaacatatta gttctcctcc tccggagtgg tgtcgtccgt agtggcggac
     5341 tgtttggtat ttgtaacgtt agaatcttct ttaactattt ggtcagcaga gatatctgat
     5401 tcttccgaaa ctccaacagg agagatatcg atggctagac cagccaactc tttggttaaa
     5461 actttgaatg actctggaat agctgggtca ggaataggta gacccttgat gatagattgg
     5521 aaggcaaaag tacgcccttc aatatcatct gacttgatag tcaacatctc ttgtagagta
     5581 tgggcagcac gatgagcctc aagagcccag acttccattt ctcctagacg ctgaccaccc
     5641 atacgggctt taccgcctaa tggctgttga gtgacaagag agtatgggcc agtagaacgg
     5701 gcgtggactt tatcgtcaac catgtggaca agcttgacaa tgtaaccaat accaacaaca
     5761 gcttcctgat cgtaggcatc gccggtacga ccgtcgtaca actgagtttt accattaaca
     5821 ttaagaccag ctttttccaa ttcactagca atttgtgctt cagaatattt ttcaaagact
     5881 ggtacagcat actttttgcc caaagtgtat ccggcataac ccaaagtgac ttcgagtatc
     5941 tgaccaagat tcatacgagc aataactgac agaggtgaca tgactaaatc gacaggggta
     6001 ccgtcggcaa gtcgtggcat atcgtactca ggcataatac gacagatgac acctttatta
     6061 ccgtgtctac cagcgatttt gtcaccttct tgaatgcggc gaatttgagc aacgtaaact
     6121 ttgacgactt tattgacacc tgggtctaat tcatcaccac tttctttatc aagtacttca
     6181 actttgatga cgacaccgcc ctcaccattt gaaacgcgga gtgatgtgtc tttaacttca
     6241 cgagcttttt ctccaaaaat agcgcggaga agtctctcct cggctgatag ttctttttca
     6301 ccacgaggct caacttttcc aacaagaatg tcatttggac caacgatggc accgaccata
     6361 acaattccat cttcggtaag tttggctaac tcggcatcgg aaacattagg aatatcttta
     6421 gtcaactctt ctgaacctag tttggtctcg acaacctgag cgacattttc ataaatctga
     6481 atgttagtta gaacatcgtg cttaaccaat cgatcagaaa ttaagaaaga atcttcgtat
     6541 gataaaccat caatagatgc gtaagctact agtaaatttc taccgataga taattcacca
     6601 tcatcagtgg agggtccgtt aataatgaca tcaccgcgac tgacaacatc acccgggtta
     6661 acgttaactt tctggttata acaagttccg tatggggaag ttctctcaaa cttgttgaca
     6721 tagtatgttt ctgtcttacc atctgtagag atttcactat gactactgaa agtatttgga
     6781 tcaatttttt tatcaagggt tatgacaact ttctcaccat caacatagga aactttccct
     6841 gcattgttgg caacaaaaga acgattcata gcagtcgcaa cagccccttc cataccagta
     6901 ccaataatag gtgactgtgg agaaactaaa ggaacagatt gtgtctgcat gtgcgagccc
     6961 ataagagcac gggtagcatc gtcatgatca acaaaaggaa ttaatgaagc agaagttcca
     7021 acaatctcgt tggggacaag gtcgatgtaa ctaatttggt cgatgggacc ttcagaaaac
     7081 tcgcctcgat gacggacagg tacccaatct tgggctatgt taccttcttc gtcgatattg
     7141 acacctaaat gagtgatgtg aacattatat tcatcgtcag catcaagata gaaaacctcg
     7201 tcggaaacgt ggtacttttt acctacttta acaacttttc tgaatggagc ttctagaaaa
     7261 ccatatcggt taacttgggc atacaaggca agataagtaa ccaaaccaat gtttggacct
     7321 tcaggagaac gaacagtaca gatacgtcca tattggctag agtggacgtc acggatagaa
     7381 aaggcggccc tctcacgagt taaaccacca ataccaacca cggtgacacg acgaagaaga
     7441 tcaatttcag cgagaggatt tgtctgatct aaaatagcag ataattggtt gcttcggaaa
     7501 aaagtgttca gtgaagaaat aagaggttgg gggttgatca tctggcttgg caatggtttc
     7561 tctttagtag aaatcaaaga catcctctct ttaaccattc tctcaaaacg agctaaggct
     7621 ggacgaatag caatttgaga aactatttca ccagaacgac gtaaacgtct gtttgccaat
     7681 gaatcgatat catcaatttg agtaacctct cctttttgga gaccgattaa tcttttgatg
     7741 gaagaaacca aatcttcttt acgaagagtc cagttttctg gattggaatc atcaaacttg
     7801 aaaccaaatt ttttgttgat tttgtatcgc ccaacagaac cgagttcgta tgtacgaaga
     7861 tcaaaaaatc tttcgtggaa aaatttttga gcattttcta agacaactgg ctcacctggt
     7921 cgcaacttac gataaaactc taagatagct tcttcttggt ttttggaaac atcagcatga
     7981 atggtgctgt taatgtaatc accaaattca gagaccagtt gtttgtcacc ataaccacag
     8041 gcagcacgca aaataatagt ggcgggaaac ttcttttttc tgtcgatacg ggcaaaaacg
     8101 acgtctttct taccaatgaa gaattctaac caagatcctc taactggacg aatctctgca
     8161 ttaaaaagat ttttgccagt gctgggatca atttcagaag taaaataggc acctggagaa
     8221 cgaactaatt gggaaataac tcctctctcg ataccattta caataaaagt cccctcttcg
     8281 gtcatggtgg gcaaatttaa gaagaaaaca ttttcttctt taatgtcacc agttctttta
     8341 ttggttaggg aagctttaac atgaatagga actacgtaat taagaccttt tcttttagca
     8401 gtatcagggg aaactggagg gttatcgata gtcaactcac ctagatcaag ttgccagttg
     8461 ttaccagtgt agtcactgat aggggaaatt gttttaatgg tctgttggag ttcgtggtgg
     8521 aggaagtctg accaggattc tttttgtatt ttgattaaat caagcttagg aaggttaggg
     8581 tattttttac cccatgaggt tctttttgcg gtaggcatca gaaaatttga tttataacaa
     8641 gataagacga gttatgttcc aaccgctcgc cgaatgtggg taattatata cgcaataaaa
     8701 gctgactaca ataggtcaaa tagctagaag aatttgaagg atgaaactag ctccggggcg
     8761 tcaccttcga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnng actcttgttt gtagggaatc ttattgccgt ttatagaagt
     8881 agcttcaact tgatatttaa cctctgaggg gcaatcaaac tggtattggt acttagtatc
     8941 tttgtgggtt ttcatactga tctttttggt aggcactgcg gtaggagata ccatattggt
     9001 tgaggagctt gcttcgcaag acttttcagt acagcttata gttaggtctt cgctgcagac
     9061 acaagtattg cagccatc
//
LOCUS       DDWP01000032            3777 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_11659, whole genome shotgun sequence.
ACCESSION   DDWP01000032 DDWP01000000
VERSION     DDWP01000032.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_11659"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1042 a          859 c          572 g         1304 t
ORIGIN      
        1 ttatcaaaag tcttttttgg taagaattta attagctctt cagtttcttc tgaagtgggt
       61 tgtttcacaa atctaactgt cgacagccat agagtatcta aatctgattg tagtttgggt
      121 atggcattac cttctgtaaa tatttcagcc atatcctttc ttatcctagt tatcataccg
      181 gcctgcggag gtatcagtat gacaaacagg ttattatcac tcggatatag tcctaatatt
      241 tttaggtcaa agttaattgg attttctcga aagtaggtac ctattctttc tgtaatcact
      301 gacaaattag catcggcgta ttctttactc cactgcagtg aaatatgata tccattagcc
      361 ggctggatga aacatttatt ctttagaact tgtggaacag aattattaac aaattggtat
      421 agttcatcgt taacatcatg gtcgagtctc attaccaaag ttagtgtttt ctgtgactca
      481 gttgctgtca gattagttag gtaaaatcca tttttacgaa cataccctat ctcttcatct
      541 aacttttttt gccaaactat aggtaactct tgatacataa gtcattataa cacttgctac
      601 gagttcacaa ctacttgccc ccaatagtct tttttatttt gctaacatta actatgttct
      661 taaaaataaa tcaagattat ttacgtagtc tgatttttgg ttttcaagac gctttggttt
      721 ccactactgg agtaatcgtc ggtattagtg ctggagtcaa caataagcca ctcattattt
      781 tgtcagccct tgtcactatt tcagtcgaag ccatgtccat ggctgctggt caatatttaa
      841 gcgagaaatc ggttcacgat ttacctcaaa atcgtcacca tgataattta atcattggta
      901 gtttgttgat gttcttctct tatcttgctg gtggcttcat ggtcataacc ccagttttct
      961 ttgttcccct tcaatacgtt tccgtcacct cgattatttt tgcttttact agtctattta
     1021 ttttaggttt tctaaaagcc aaacttttca ccggcaagat ctggcgcagt gctattgaaa
     1081 tgctggttat tggtggcttg gctacattaa ttggactttc cgttggctca ctcttcagca
     1141 caaccatcta ggtgctgatc ccatctcttc atgtaaaaaa tcaactctca ctaacgttgc
     1201 atcgtcgttg tctttaagtc taataacctc taaatattct gggtctgcta gaaggtcttc
     1261 tcttcccttt tccaagaacc ctcctccttc tcccactggg tatttcgccg aatcatctgc
     1321 caaagctttt actgccgatt ctaagggtac accttttttc aggtaaggtt gcaacgctcc
     1381 gtcagagaca ataatcactc ccaacatatc gggtgtaatc tctgctttat tttcgtatat
     1441 caagttattt tcttccaatt gtggcatgcc gtccaatatt ccgcaaccat ccggaacatt
     1501 cgtttttctc ccaaatgaat ccactagctg ggcttttacc aatgctttta gttcaggtct
     1561 tgccaaaact tggcgggggg ttaatccgta ctctcgagcc aatgattcca ataaagccgt
     1621 tgtctcgtca tcaaacttct gattctggtt gaaagttaat atctcgaccc tgtcggtata
     1681 tattgccact gctgtggtgt cggccacgtg agccactgtc accactttat tgggggcgtc
     1741 aatcaaacaa aagcttgccg ccattcctgg aacacttaat atatcctgat aatttatccc
     1801 ttctacctta tttaaattct cgaataacca acggttaact gttctcatta cgtctcctgc
     1861 ggtcaatgtc tctctgttgc acagttcgcg atactcttct gagttcacaa actctaaact
     1921 aactaggtga gaaatgtaat atgctcccga cacacctcta acttccaatc cggcaattgc
     1981 cttgcggctt gaagcaccat caagcactga gacaagttct ctacccttac ccattggtat
     2041 tacgaacggt gaatcctcat taggtcgacc agaaaccgat gaacccctag tctcttgtct
     2101 aattactacg cccaaatcaa ttaggttaac tctctttgta aaagagtgtt ctagcacgtc
     2161 ttctatttgg tcaggtgaaa caaaccaata cttctgacct ggtactgctt ctgcaacggc
     2221 cattatatta ggaagattgt atcacaacct ttgttacgta taagatatat aggtttttct
     2281 tgcagtcaga tccaccttgg taccaacccc atgtattctt tatacgtttt cccgtatctg
     2341 gctatcagct gaggttcttc aataaaaatt agatacgact gcaatatcag ccaacctaca
     2401 aaagcatacc ctaaaagcag caggcttccg tgccagacaa atatccccag agctactaat
     2461 acatgtccaa catacattgg atttctcgtc ttttcataaa ttccattttt aaaaaattta
     2521 ccctcggttt ctgtaaacag tggggtcctc tctccatgtt taaaaaatat tgaagaacag
     2581 taggggaata gtatcaagcc tgctaagaaa attatcgctc ccaccacctc ttgccccaca
     2641 aacgatattt taaatatgtt taggctttca ctaatggaaa tcaaccacat cgggataact
     2701 acataaataa aaggcactaa aataacagaa gcaataatgt atacaattag tttttttaca
     2761 ctaactttca taccagatta tacacaccac ccacgttatt tatccaactt ttttcttatc
     2821 atcaggtaca accgaccatg atctcgagtg attgccaatg tggcttcata atctccgtct
     2881 ctccatttca ttaatccagt tttttcatca taggaatatt ctggtttatc tttatatagc
     2941 tctttatatt tattttccag caataaaaat tcatctatat cttcgtcatt gtttataacc
     3001 gtaaaatact ttgtgtattc tatctcccca cccttctggt ttagtagtcc gtaagacttt
     3061 tcatgttcca ccatgattgt atccgtttct aaaatattag gaaaatctga gaaataccta
     3121 ttcaaaccct catcgttata ggcaggtagg tggacttgta tatatcctgt attccccgat
     3181 ccataatgag ccgccgatac tttatattgt ccaattttcc ctctcaccat atctggactt
     3241 tcaaattctt ccactatctg actttcaaat aaatttttat aataatttaa gaattcttcc
     3301 tgagtggcat cagaatataa gaccacattg taataagcaa aactactctc tccaaaaccc
     3361 gacaaatttt ttggatctgt atttacaaat attttatttg agctaacttt atcgagttta
     3421 tagaggggaa catcctttat aggaaatcca tccagcaaaa aactattctc ttttttcacc
     3481 tcttccacct tgttttcagt gacgtttgtt ttctgagtac tcctagaatt aatcaccaga
     3541 acaaccccaa gaatagatat tataaccaac actatcccaa tctttttatt catcatagta
     3601 gattttagca gatgtctcat tcaccatatt tttgcttaaa agtatcagca ctttttattt
     3661 cactcgcggc tctaatacct ccacctgact catcccaatt ctctttgttt gattcccaac
     3721 tagcccccat attcttaata tatgacttgt aatcatcttc atcgtaaaat gtcattt
//
LOCUS       DDWP01000033            2525 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_47078, whole genome shotgun sequence.
ACCESSION   DDWP01000033 DDWP01000000
VERSION     DDWP01000033.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_47078"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          736 a          344 c          654 g          791 t
ORIGIN      
        1 ttgaatggtg gtggattagt tttttaaagt caaaatgggg atctgtcttt tgaatatttt
       61 tgataacctg ttgtccgaga ctaatgagct tgtaatcaga aaggctggca attttaagag
      121 aaggaaaacc atgctggact agagaaaagg catcacctgg gccacgagtt ttgagatcgt
      181 attcggcaat tttctgacca tcgttagtag agacgagcat ttttaatctg ttaatagctt
      241 tgtcattact agattcggaa aaaagatagc aataactctg tagatcagag cgaccgactc
      301 gaccgcggag ctgatgaaga ccggctagac caaatctatc ggcggattgg ataataatag
      361 ttgtggcatt ggggaagtcg atacccactt caataatagg agtggtgatt aaaatattga
      421 ttttgttttc ttgatatttt ttgagaattt tatctctgtc gataggagga attttgccat
      481 gaatgaggcc taacttaaaa tctggaaaaa tgtctttttg caaatgagta aaaagctcgg
      541 tggcggattt gactgaagtg agagtctcgg atggttcgat aaagggacaa actataaagg
      601 cttgttcttt tgtatttttt atatgtttgg cgagccattg ataacaatcg atttgtttaa
      661 taggtgggac tagataagtt ttgatgggta aacgatttaa tggcggagca gtgatagtgg
      721 agagttctag gttgcccatg atagttagcc cgatagttcg gggtatgggg gtggcagtca
      781 tggtcaaata atgaggaggg gtaagagagt cgattaagaa tgttcgctga gcgacaccaa
      841 atttgtgttg ttcgtcgacg ataagtagac caatcttgtc tttaaataat tcttttttgt
      901 aaatggcggc gtgggtagca attattattg aaccattagg gaatttaact attttcttat
      961 ttccagagag gaactttatc ggaactttta gaaatttttt gaaggtggcg aggtgttgtt
     1021 gggcgagaat ttcagtagga gcaactagaa gcgaagtgct tttatttaaa tgagctagat
     1081 agcaggctag aatggcaaca atagtcttac cagaaccaac atcaccttgg agaagacggt
     1141 tgctgggatg attggagaga aggtcagccc ttatctcttg ccaggctttg acctgagcat
     1201 cggtcaattt gaaaggtagt gatttgatta gactttcaat ttttgggtcg tcgaggaatc
     1261 gttgatgagg cgttttactg gcccaagatt gtttatttaa taaagattga cattggagtg
     1321 agagaatttc atctagaccg agacggatag tggcttgttc taatgttttt tcgtttgatg
     1381 gttcatggat ttcttttagg gcggtggcta gatttagaat attttcttga ggtgggagaa
     1441 aatcagtgac attggcaaga agattgttga tgttttgagg aatggttttg cgaaaccagc
     1501 tgctacttaa acccttggtc tcgggataga tagcaataat tttgccagta ttgtgttgac
     1561 cgctgacggc tccaataata gttggtttat tctgataaag tgagactgtg ccagcaaaac
     1621 tgtagatgtc ccctactaca aaattcttgg aaagataggg ttggttgaac cagattagat
     1681 tgattgatcc gctggagtcg gccacggtgg ctatctggat atttttgtga ctgcgggtga
     1741 agatattact gaattttaag agtttgccgg tgatggtggc ggtagagttg atctcgagat
     1801 tgtttatcag ggtaatatgg gaaaagtcga tgtagcgatg agggaaatgg tagaggaggt
     1861 cagagggggt ggtgatgccg aggtgttcta gttttgctag ggtttttggt ccgatgcccg
     1921 gaatgattga gagggagagc atagagtatt ttaactcatt gattgatagc tatttctatt
     1981 tcaatccacc ccttaatccc ctcctttgac aaggagggga aataagaata tgtttcaatc
     2041 cctctaaatc tccctttgac aagggagact tgagaacgag tattattaat tgtgaggaat
     2101 attattcctg atgcaattca tacaaaaaga ggggtagtta gatattggag tgatttaaaa
     2161 gaagtggcta gaagaaacag agttaatcct accgaagctg aagaaaaaat atggaaagaa
     2221 gtgttgtcta agaaacagac aggttttatc tttttgaggc aaaagccatt acataggttt
     2281 attgctgatt tctactgttc tgaattgggg ttggttgttg aaattgatgg agcttcacac
     2341 aataaaaagc aagatactga cagattaaga gataaatttc tccaatgtct aggtatcaag
     2401 acaattaggt ttactaatga agaagtatta aatgatctaa atgatattag gcagaaatta
     2461 tctcccctct tgtcaaagag ggggtagggg gagattgaag ttatgttatc atttataggt
     2521 gaaag
//
LOCUS       DDWP01000034            7155 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_15163, whole genome shotgun sequence.
ACCESSION   DDWP01000034 DDWP01000000
VERSION     DDWP01000034.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_15163"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2151 a         1055 c         1699 g         2250 t
ORIGIN      
        1 ctagggctaa ggctttggaa taaagtttgg caaagtccgt gtctttaaca taaccaagaa
       61 attcaacttg atcgggactg gtgatttttt ggagacggtc gcgaaaaaca gaacgagcac
      121 aaattatctt taattttagg ttaggcaaag attttagagc ctcgataatg acgttgatgt
      181 ttttgtgagg gtaaagattg ccggtataaa ggatatatga tttaggattt atgatatatg
      241 atttatgaat agaaaaaaaa gaggggtcga cggcttcatg ggtggtggtg actgagccat
      301 gctcgactcc gagtcggttg attatgtatt gtcgccaata ttcactaggg acaataatgt
      361 gactggtggc caagactatt ttagtcatga gcatatatgc ccagtattta ggccaatata
      421 gtagaggtga gcgggtggtg gtggcagagc ccttggaaaa gtgtcggatg agatcgtgga
      481 cagtgacgac agatttgcca aaataaagaa ttggtttatc aaggtgggtg aggtgaacta
      541 agtcaggttt taattttttt aaaagtatgg gtaaaaatat ttgttcagcg atagaataat
      601 gacggagatt ggtgatggca tattgataat tgtcttttaa gtcagaggct atttcggaag
      661 ctaactcagg atagacaagt agggtgaagc gatagttttt aaaatctttt tgatgaggta
      721 ggctgaggag taaatttttg gtgtaacgac caagaccagt gtgtttgggg ccgtaaagtc
      781 tggcgtcgat aactatgtgt ttttgtttga caattttggt cattttttgt ataattttcc
      841 taatcacgag agagaaacga gcttgtaact acgtgttaca ctgttacgcc tctcattttt
      901 ttgtctatac tccaactttt tgttttcgaa ttcaactttg aacttgtatg tttcaaattc
      961 ttttaataga gatgaagctg actttgtgtt gggaatgata attaatttta gtttattttt
     1021 ttcttgtaga tatttataga gacagtgaaa atcaccatca ccagaaacta ttatagcctt
     1081 gttgtactta tctactaatt ttgcggctga atataaaact aattcagcat caacgtttcc
     1141 ttttgtttta ccatttttgt ctgttacggt tggtttgaag acgatttcat atccgaattg
     1201 ttttagtttt ttataaagat cttcgttctc tttgatatat ccaataaata gaatggcttt
     1261 agaaactctc catttgtctt ttaaaaatat aaagaacttt ttgtaatcta atttccagcc
     1321 tttgtaaact agtttgccat ttttgtatat atcgttacta agacctagat ttaagttttg
     1381 actatcaata aaggcataag ttatttcagt tttattcata gatatttggc tttgaagatg
     1441 cgatagaact cgagtaagtc gatgccgcgc cagacaatga attgtagtaa aggtggataa
     1501 ttttggatta agttttcccg gtaaaatatt ctcatggcat tagttgtagc cttagctgaa
     1561 cgaaccttgg tttctcgagt ggcggctgat ttggcttttt taatacctga agatataccc
     1621 tgataatggg taatctgata gtcgggaatg aagaacatgg taaagccgtt tttcttaatc
     1681 tgataactaa agtcgatgtc ttctccatac ataaagtatt tctcactcca ccatttgcac
     1741 ttgtccccaa ctgatctttt gagcattaga aaagcaccga cacaagaatc aataggttgg
     1801 atctttgagt agtcaaggtg acctaggaaa taaccgttaa agatctttgg tcgaggaaat
     1861 atttttgtag aaaaaggcag aaaaaagtgg caaaaggcat tccatggggt ggggaaacct
     1921 cggtgacatt cgggttgggt cttaccggtg agagctagga tgacgtcgca ggtggcagag
     1981 tcgatgtggg ggtgtgtttc aaaatagtca accattcctt gaaaagtatt gggtttgacg
     2041 gtggtgtctg gatttaaaaa caagacatat tttgaattag ggtcggaagc ttttaagcct
     2101 cgattattgc cagcggaaaa gccaatattt cccttgttgt cgtgaaatat aaaattaatt
     2161 tttgggtgag agtgtttggc attttgaata ttttgaaatg aaccatcagt tgaagagttg
     2221 tcggccagaa ttacttcaat tctatctttc aatttggagc ggacaataga atcgtaaagt
     2281 tgtgagagat atttgttggc gttgtaattt aggatgatta ccgaaagtat gggtgaacta
     2341 ctttttgtgg gtgatttggt cataaatttg gtttagtttc tcgacgctgt ggtcaaaaga
     2401 gtaattggtt tcaattagtt ttctagcggc gtttcccatt tttttgcgat aggtcttgtc
     2461 atcaaataat ttgtagacat ttttgagtat ctgtttcttg ggacaaacaa tggcgtgatg
     2521 ataattttca attttgatgt tacccatacc tccttcagga ttggtaatta cgggtaagcc
     2581 aaatgtcatg ccttcgagga atttgttgcg tgtgcctccc ccactgccca tgggggcaag
     2641 gagtacccag caatgttcat agtaatactg gggatctttt aatccatcaa tatcggcttc
     2701 ggtaacgata atgtcggagc tgttgtagcg attaaaaaat tctggagcat tacgaccaac
     2761 tataaatagt ttggcgtcgg gatatctttt cttaatgggt gaccaatagt cagaaataat
     2821 catttcgact gattctcgat tttgcatcca tttcatgttt gaggcaccaa aaagaactga
     2881 aggataggta gactttttgg ttttttgtgg tagcttgaaa tattctgggt cgacagcgtc
     2941 ttcaaaaacg tgaatgtctt ttcgtccagt agatttgata acaaaatctt tatcttcgtg
     3001 actaaacatt aagacagtgt gggtttcttt ccaatatttt gtctcccaat attttagttt
     3061 gaggacatca agccaaagaa gtggagcggc taatcttctc cagccagtta ggctctcgac
     3121 ataatgttgg tatatggcgt attcaatagt gacatcaacc ataactaacg gaatatcagt
     3181 tttggggata ttgggcatgg ggtagagaca gtcacagtga atcaggtcgt attttctagc
     3241 ttttatctcg ttaacgatag cattgcgaaa atcttcatta tgataattta ctagtagaaa
     3301 aggatagagg ctaaatccgg cagaaagaat atttttgaga ctccaagtgt tgcctctctt
     3361 aacgataata actttatggc aatatttttc gacctcttta agaccttctt cgttgcggct
     3421 ataacaaatg agagtaatgt tgttagtttt tgataaatac ttgatagtaa agaaagagtg
     3481 tctttgtcca ccagactgat gtagtcgagg aatgtaagga gtaatcatga gaatatttct
     3541 cttgtggttg gtcatatttt tttggtaaga actgaataat aaattcccat cataatccag
     3601 aaagtatagg cggtcttgga agcctcaaag gcgtcaatta gagtggcgtt aagcaagaaa
     3661 gtaatcatag aagcaaagag tataagagat agtttgttgg agttgtccat ctttttaaca
     3721 aatatttttg gtacagtctt gacggtgaaa aagataaata tggcggagaa agtaataaat
     3781 ccgagcagtc cgctttcacc aagattgcga agataatcat tgtcagtggc tagagtaatt
     3841 gagccaaggc cagtacccaa cagcatattt ttgcggtagg cggtgatggc tctaggccat
     3901 tcggcattaa aacgaatttc tccggaacga gctacaccag catcagcgtc tgggggtatg
     3961 tactcatcaa ttggtccgtg tcggataata gtcgggacag gggtggtttt gccaccttta
     4021 actggctgag taatagtctt agtaactaca gcaattacgg ggacagttgg tgtagcagtg
     4081 ggttgtggtt ttgaaggaaa gaattgttgt tgaagagcag gaatagtagc cagaagacgt
     4141 tgatttagat ctttagaagc gaaaatacta gaagcaacta gaatggtaac tggaattatc
     4201 cagagatatt ttctgatgat gataagacag agagacatgc cgacaaaaaa ggagaagatt
     4261 gatacgcgtg aggcagtcag ggtgagcata tcgtagctaa atatccagat gattagactg
     4321 agaattttta gccagatgtt tttttgagtg acagcaacgc cgccaataat aattagcata
     4381 acgctgaggt agatagccag gtcgtagtga ccggcaaagg ttgagcttag tcggctccag
     4441 acatcaaatt gaagccattg gcctttggag aattccgaat tcatagtgga gataactggg
     4501 aaacttaaga atttttgtcc ataaccatag aggaggactc cgacggtaga gattagagaa
     4561 aagatatata aatgtttaat atcttcgtat tttttgatgg cggatatggt taagaaaagc
     4621 agtgacatat attcaaaacg acgcagaaga tggagaagaa gtattttggg tggatcagtc
     4681 tgatagatga ggatcgcagt gacgaagcta gccactatgg tgataaagta ggcaataact
     4741 aatttagtaa ttggttcttt gagtatagga aagcggtgtt tgatttgatg aataagccag
     4801 acgaatagaa tactggcgac gactatatcg tcaagacgga tggcgacata agtcccggtg
     4861 acatgagcca agggaaattt tggatagaga gggataaata gaactagtac agccagaagt
     4921 ggtcgaagta aaaatatatt cttaaacatt tttgattaat ttgtagacgc taaattggag
     4981 aaaacgattt attttaacca ataacttgac tactggatat tgccaggctg gtcggtgaag
     5041 acggaagaaa aggagtatgc cttcgtattc tcgatcgtag attagttgtt tgttttgaga
     5101 agatttacca ccaaggtgaa taatttgtgg tccgattaaa tatgaaaatt tatcttgagg
     5161 gtatttttga gataagcgat aacacatttc catttcttct ccatacatga agaactctga
     5221 gctaaaaccg ccgacagatt taagatgatt ggtgcggata agcatgaagg ctccggtgat
     5281 ccaatcttga gaatggtcgg ttaaatagaa tttgtcgtga gtatagaaat gaggagtgtg
     5341 agggtgaaaa ggcttgataa tatgattgac aaaaggtagg tcgtcaagac caaggcacca
     5401 ggtagtgatg ttgagaagat tgggaaaata tccgcctgag gcttggatag ttttgtcggc
     5461 attaagtagt tgaccggtgc aaccaatact ttcgggatga ctacaaagcc aatcaagaga
     5521 ttggctaata gctgagtgga gaatgatagt gtcggagttg agaagaagaa tgtaattgcc
     5581 tttggcaatt tccaaacctt gattgttggc tttaccaaaa ccaacattgt ctttgttggt
     5641 aataagagtt agaggatatt tggttttttt aatttttgaa acagaatcgt cgtgggagtt
     5701 attgtcgacg acgataattt caaaaggagt atcttttaga cctttgtcag tataaattga
     5761 cttgaggcaa tcaacggtaa tatcggcagt attgtagcta acaatgatta ttgaaagtac
     5821 aggtttaatc acgtgatttt ttaaagagta agtttttata aattaaaata atcgagataa
     5881 tagctgcttc aagtacaaag agggtgatta gtctttgagc aattgaagtg gggaatactt
     5941 taatggtgat cttggtctga tactcaatat tgttgtgaag taatttattg gcataacgtt
     6001 tttctggctt ggtaaaaaca tcattttgcc attcgtagcc gttgacaaaa atagattctt
     6061 taaaaggtag aataatttct tcaagataat atgattgggt ggtgtcacga attatttctt
     6121 tagctttctc tggaggatga ttgagctgga ttctaaaagg tccgtggtag ttggctttat
     6181 agaaattaat aacttctgtg cgagtaagat tgccaaagta ggccgagaca tttttaagct
     6241 gggtggtgtc tcctggtaaa tcagacttaa cagcattagg aatgtctttg agagttgggg
     6301 tggggctaag aagataggtc aaagcacaga cattaaaaat caaaaataga gtgatgtaaa
     6361 atcttttcat tattgaaata acatcgctcg accgagctga taatttggat ttctattgcc
     6421 acggtctttg atttttttgg ctgggactcc accccaaact tcaaagtcgg gaatgtcttt
     6481 agtgacgacg gcgccagctc cgacgacggc acctttgcca atagtgatgt tgggaagaat
     6541 gatgactctt gggccgataa aaacatagtc cttgatagtc actggcccaa aagaattacc
     6601 ataatcggtg tcgatatcat gttcgtcatt atagataaga acttggctag cgataccgac
     6661 atggtctccg atagttaatg aagcacgtcc atcgagaaag cagtgatcgc cgatgataga
     6721 atcatggccg atggttatgc ccgaaggttt aaagaagttg gcgccgatat gaatagtgga
     6781 agagaagggc attttgatac cggaggccag ataaaatatt ttacgaatag tatggatagg
     6841 tatataaccg acgattcgta ggactagtag ccagaactcg agagtaatag tagacagtcg
     6901 gttggttatt ttgggaataa ggttttttgg agtcaaactt tgaccgacgt gctcattcat
     6961 agaatgatta taacctatca aacagctttt gaagggagcg ggcagagttg atccaggtga
     7021 agcgtgagga ggtggaaata ctttttttaa ttaattgatt tctggtagtt ttgtcttgga
     7081 ttttttccag agcttgaact agtgagtcga tgttggttgg gtctataaat aatgcagaag
     7141 agccgagtat ttcgg
//
LOCUS       DDWP01000035           13910 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_22432, whole genome shotgun sequence.
ACCESSION   DDWP01000035 DDWP01000000
VERSION     DDWP01000035.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13910)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_22432"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    12311..12360
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4119 a         2909 c         2364 g         4468 t
ORIGIN      
        1 tttggtaaga caagccgccc acatataatt tttttaccat taaagtaaaa attaataatc
       61 tgtagcgacc tcttaccagc tattcactga atcgaccctt ctctacaaca ggtattatac
      121 acttatctgg ccaaagtact accccttgac acgtttttag actaatacta tagttgtagt
      181 agttaatttt taataaatta tgaaatacat cattatcatt gttttggtag ttattgctat
      241 tggagctagc tttatttttt ctccaaagag ttctgttgtt cctgctactg tcgagactgc
      301 agttgataat actcaagaag atgttcaggt ctttgatatc aaaggtcttg actatgacta
      361 cgatattaag gaaattagag ttaggcaagg taccgttgtc cagataaact tcactaatac
      421 tgaaggattt catgatttag ttattgacga atttaatgtt gccaccaagc aaatacctgt
      481 caatcaatcc gaaaccatta cttttgtagc tgacaaggct ggtactttcg aatattattg
      541 tagcgtcggt aaacatcgtg ccaatggtat gtggggtaag ctgattgttg agtagtttta
      601 gggtatacta atcatctaca ttaattgctt tatattatgg tgactgaagt cgtctttgac
      661 atcgaaacta aaaagatttt tgacgatatt gaaggtgaca atcctgccga tctaggtatt
      721 tctattatct ctttatataa acgtaccctt gatgaaaact taaatgagat tagtggagaa
      781 atgatcagtt tctgggaaga agatttttct aaaatgtggt cgtattttag tggtgccgat
      841 cgcattattg gctttaattc tcttcatttt gacgtcccgg ccatggctcc actggcccca
      901 tatgatttta aaaagctaaa tcactttgac atcatgactc atattaagga tgctttaggt
      961 tttcgtgttg gtcttaacgc cattgccaaa gaaactatta atcatcagaa agttgatcac
     1021 ggtttaaatg cggttaaata ttgggccgag catacaccag agtctttatt aaaactcaag
     1081 acctattgtg aagccgacgt tatggttacc aaggaagttt acgattacgg tctcaaaaat
     1141 ggtcatctaa aattcatgga caaatggaac actgtccgcc aatttccagt caacttttcc
     1201 taccccaagg tcgacacttc aattcccgtc caagtcggat tgttctaata ttacgtcatt
     1261 gcgagcctga gtccatcgaa ggcgtggcaa tctttacaaa atgagtctga agtctatcta
     1321 acctttagta attatcggcc atcgcgttat taaagtttgc ttcgtcacta tctcaactgt
     1381 tcctcgcaat gacggtctat agcactcttg aatcttcttc aattttttct gtcttattca
     1441 atatatacac atctgcccga tattcgtcat gacccacaaa ctcagcccca ccttttaata
     1501 tggcctccat tgggcgacag cttcgacagc caccctcacc ttcaggacat ttaaacactt
     1561 ccaattgtct ggctagtttt atttttctag caatctttag aattttttct tcagaatctt
     1621 ttacttcggg caactctttt ttggtcagat tatcgttact ttctaggtac caataccaag
     1681 ccccatcaac ttttcttttt tggcaattgt ggacaagcag gctatagatc ggtaattgca
     1741 ggctttcccc ttcctcttca ttcttactgg ttttaaaatc aattatttct acgctatcac
     1801 tttctggatt gtattttagc caatcaattt tcccgcaaag aattatatta tcttcttctg
     1861 acagccaata ataagggagg tccatattta tttttactgc caattctgcc actggcccag
     1921 ggttttttat tactcgtctt atcatttcct cacctctttt tttatattta tattcagtat
     1981 cagtatctag aaatccacca gatttcccgg acactttatt ccaagctctt tcatatttat
     2041 ctaaaagtgg ttctttaaaa cgtaaatcag tttttatttc cgacaacgat tcaattactt
     2101 catgaactgc ttgtcccaga gccagcggag gagacattag ttttacttta tgtccagtcg
     2161 ctttatcttt ataaatattt ttcaaaaaat atgctcttgg acattccaag aaatttgcaa
     2221 ttgaagaatg tgatacccaa actgcgctat acttatcttt actcatacac aatgatatct
     2281 tattttatat aaaattcgta attcgtaatt cgtaattcgt aatttttaat ttatatacat
     2341 gccattaatt agactaaata aatatctagc acaattagga gttgcctccc gtcgcaaaat
     2401 tgacgagata actattggcc gtcgaattac tattaatggt cgccttgcca ttcctggtga
     2461 caaggtcgac cccgaagtgg acaccgtaat tgtcgacaaa aaaattatta aacctacccc
     2521 tcaaaaatta gtttattatg tcctcaacaa acccaagtat gtccttagta gtgccagtga
     2581 cgatcgtggt cgtaaaactg ttattgattt agtacccggg acaccacgag ttttccccgt
     2641 tggacgtcta gactttgaat caaccggttt aattttatta actaacgacg gcgatctggc
     2701 cttgcgggtt acccatcctc ggtatcatct ccctaaagtt tatttagtca ccgtcattgg
     2761 taaattatct tcagacaaat taaaacaaat gggtgaagct gaaatttatt cttctcaatt
     2821 taatcaaact acaattaaga taacactgca tactggtaaa aaacgccaaa tacgtttact
     2881 ttgttctagt ttgcatcttc atgttgttga cttacatcgt atttccattg gccctatcga
     2941 gataaaaaaa ttagctcccg gagaataccg agagctaact ttagatgagc taaaatctat
     3001 ccgactttaa cttcgatttc ttttggtttt gattcttcgg caacttttgc cttaatggtt
     3061 atcactccat tcttgacctt agcctcaact gatttggcat caattggacg ggggagggag
     3121 gtcacataat caacagaaga tgtcatctcc cattttttaa ttacttttcc ttttttacga
     3181 tcttcatctt tttctgacac tgtcccatat atatgtaaca taccatcgtg ataagttact
     3241 tttacatcag tcggttcgag ccctggtact ggcgcttcga ctgttacctc agtctccttc
     3301 tcatctacat agacatttat tccagagttc atctgaaagt caggccaatt gtcttcttcc
     3361 caagaaaatg ggcgataatc aaataacggt tttaaaggta gatacctcat atatcattct
     3421 ccttaaaata aattattaat taagaatcca ttagcagtat aatactcaca ctgccagcat
     3481 gtcaagatgt caaatgccat tatgctaaca tctattatga attggcaatc ttggagtccg
     3541 ttaactgatc cattaattaa gtttcccatt cgtaactttt gccctgttaa gcaggtttct
     3601 ccacccaaca taaaaccatc aggtttttat cttaaaccaa ctatctctgg ttgctgcacc
     3661 tggtatcgtt ttggtaaaaa tatcacccaa gagaaagttg ttggtttcgc taccaagaca
     3721 aagagaattc atcccgagat aaagtacgtt ttgattgatg atggttggtg taattgggga
     3781 agttgggatc agccaaaatt tagcattaaa gatactagcc aagaactgtc taagatgggg
     3841 ttagaaactg gcttgtggct agccccattt ttcaagacta ccccgccttt taatttaaga
     3901 aaaattgttg atttttctaa ggcagacaat ctggcttata tccactcttg tttaaagaca
     3961 attattgaag actggggtgt cactttgcta aaactagatt ttctctatgc accatacttt
     4021 caacccggcc tccaagacga tactcttccc catcagcaac taactaattt atttaaattt
     4081 attaaagata gttatcctca tgtctatctt atggcttgcg gttgtccttt tgagccagcc
     4141 aaatatttag ttgactcaat tcgaattagt gacgacatta ctgtgccttc tctctactct
     4201 tatggtttta ttaaaaaact tgtccacaat catcagcaca atctgttgat caagaagtgg
     4261 tcttcttgcc aaaaccttgc tgtatatttc aatctcgacc cagatgtctt acccgaccag
     4321 aatcttgctg gcttttccga tcttcagatg aagcagctag aagagatatt tacacaatca
     4381 aaagttcagt tttatggttg agccactttg tgataaagtt aactcaatat gctaaatcaa
     4441 cttaagaaaa attggttaat agttatctta gtctcagtaa ttatttttct cgctagtcgt
     4501 aacttaagcc ccataccttt gtctttaccc tcttctggcg gttttgaagt agccagtagt
     4561 aagatgatgg tttccgatat ttcttaccct ggtcgtcaag tcgctccatc agaatcatct
     4621 aatcgactaa ttatccaaga cactagtctt tctcttcaag ttaaagatgt cagccgacag
     4681 ataaaaaata ttgaagatgc aaccaaacaa ctaggtggtt tcttggtcaa ttccaactta
     4741 tcaaaaccag aaggtgcggc ttctggtagc atcactgtcc gagtgcctga ggctaagcgt
     4801 gccgaggctt tggatacttt taaacaggat gcagttaaag ttgtctccga gtcaatttat
     4861 ggtactgatg ttaccgatca atatactgat cttgaggcac aattagcggt tcttaacaaa
     4921 actaaatcca agtttgaaga aatattggat aaggcctaca gtgtttccga tctaatgaat
     4981 gtccaacagc aattaattaa tttacaacag caaatagatt ctgttaaagg tcaacaaaaa
     5041 tattacgaac aatcggctaa gttatctaaa gtcactattt acctgtctac cgacgagcta
     5101 gctctcccat acgctccgac taatgaatgg cgtccagccg tcgtcctcaa ggaagctgtt
     5161 cgttccctga ttggtaatat tcgtggtctg ggtaatttat tgatctgggg tgttgtctat
     5221 tcaccactta ttgccatatt gatagctatt tatctgtggt ataagcgtag aaaatagtag
     5281 tcttaccata attccaatcc gtgaatcaaa tgattccaaa gttactggtg actaattaaa
     5341 tcacatcagt ccgtgagtaa tatttttccc acaattcatc atactgttca tgagtaaggc
     5401 taccttcgga taagtcttca ctagccacgc ttaatgccga caacgcaccg actggagttc
     5461 tataaccacc aatttcgtta gagaaaaatt gaaaagtctc agattgtacc tgtctcactc
     5521 tggcgttgat catattgttg aactctttac ccagttcctc ctctatacca gtattctgca
     5581 tgtgccgaat tgcacgaatg ttatcagtat cactgataat ttctttaagt tctgagattc
     5641 tttgttgatc aaacattttt atttctttaa ataactaata ttttcttcat caacatgtct
     5701 aattctcacc ccaatatccg tatttgtatt ccatacctca gtttcaatta cctctagttt
     5761 tagtccgcct cgtacagcaa tttctctata actatattct gtttttgtac taccactctc
     5821 ggtatcaatg accttgatgt tgcttcccac atttctttta tagacttcca gtgaagttcc
     5881 caactcgtga acatgtaacc ggccaggtga ctctttacca gattgaattc ctggaactaa
     5941 atactgcagt cccacaattc tgccgttgct agaatcgtgt ttgattattt cagcaccagt
     6001 gattgttgcc tcaccattat ctttttttaa gtattcaatt ctcgctgtca tcttattttt
     6061 taactgctaa ctcaataccg ttttccgttt tccatttact ccaaaggttg tagtagttaa
     6121 aaacatcttc gttagttacg acgccaaaga ctccagaggg tgtcaggcta ccaagataac
     6181 taagccattg gcaacatttt tcgggttgag attggtcaag tacaaacact tcccgaccaa
     6241 tcaagtccat aatgcctctt gcctctttct cttgctcgat gcgtcgtttt gcttctcttt
     6301 cagatgcaac aatcatgatt gctcggtacg ggtcgtcaac agactccatc catttttcta
     6361 gctcttcagg ctgaaagcca ttcactaaat caattgctac ttccctaaag taacacttcc
     6421 aattctccct atcaatctcc cacagccgat gttccttgtc ccatgtttct tggtagttat
     6481 ttgcaatttc tgcggcactt gcttgaattc ggcgtaaaag acattctgga acggcatcaa
     6541 ctccgttttc ttctttaagt gttttgattc tttcagtaat atacgactta gtaatttcct
     6601 cgtgtgtagc caacttaaat ggtggatatt ctgggaaata ctcgtgtgtc atattatttt
     6661 tcaatattta atctctcatc taaatcaatc actttgaccc tatcaacccc tgacaccttg
     6721 acagcttcag ccacgatatt aatcatgttt tttgcaatat cactccaagt acaatcgacc
     6781 gtatgttttt gttcaagtgc ccaagggaca atatcttttt cccaagtgtc tccgtactcg
     6841 tccaagaaag ttgctccaac ccattcatat ttcatatatg ggtcgttagg cagcattctc
     6901 aatacacttg tgtcacccgc ggatccctct tttaagaatt cgacgaatga attttgagtg
     6961 ataccccaca gccatcgagt tatctttttg tcgggaatat ccatcatagc tggcccaccc
     7021 gtaccataac cggcaaatat tgagccgacc aaagaaccaa ctgttgtgac ttgatctggc
     7081 tctagtttcc ttaaatgaga tgctgcttgg tctatcgcca aatctctcgc tgaatttaca
     7141 tttggtttcc attcttgatt gcatgttttt tctgtcatat gtttaattta aacctttaac
     7201 tttttcagac aattttgagc gaatattagc taatctggct tcacattctc ctccgcggtc
     7261 atactgagtt agtgtgtcaa aaaaaccaat aatagctgga tagttggggg tgttcatttc
     7321 atcatcatca aagacggaaa agaggcatcg acgtagttcg tgggagaagt ttgcatcatt
     7381 taacgccacc atacgtgagc gtttagtaaa tgagacaaaa tcttccgctg tcacttgctc
     7441 gtcacttttg aataaccaat tatcggtcgt caaaatgaaa gccacaggat cattaaacga
     7501 aaattctcta gcttttacag aaggcatttt ttctttgttc atagttattt tttaaatttc
     7561 taattgctca taatataact tataggacta aacgttgcct gacattatct gacacagttt
     7621 tcagaacgct aaagctaaat cagctcagat tgtgataata tttagtcaat ggtaaagaat
     7681 caaacatctt atgtttatcg aataagacaa actactgaaa acttaaaaaa gataatttct
     7741 agtcggacaa acgtctacta tataggatta cctgagtgtg gtgtcaaaag aaccatctct
     7801 gaatcttttc gttcgcttgg ctccgacact ccgctttcca ttgtcgattt gtctttaatt
     7861 tctcaagaca aaattgagtc acacttcact tctttattaa atacaaacac ccccagcttc
     7921 ttactcgtcg aaaatttacc catatattct tctactgggc aagatatttt tacactaatt
     7981 gaaagactcc gcgatcaata tcccaagaat actaactata tttatcactt caacaccaac
     8041 ccttactggt acggcattcc tgagtcatca cgacaaattt tttatttttc tttatcaact
     8101 ataaacgaat attctgacct ggtcgatcat tttttaaaac gttttcaaat aaaagtcagt
     8161 caggcacaag ctaacgttat ttataaagtt actggtggcc atccttatct tactaaaaaa
     8221 gttttcgagt taagtctaca agagttacca gctgatcaaa tttctcaaaa acttcttgat
     8281 atatcaaatt cactaatcag tcgactgcac atttctgaag tggctcgatt aaagaacctt
     8341 tcttctcaag acccagaatt tatcactcat tttcaaaaac ttggcttaat taatgataga
     8401 aatgaattca ccagtattgt gattgccgaa tcaattcgag tgtttaagtc actaaaacaa
     8461 cttttaattg aagagaatac tataagacta ggggaaagtg acttaaataa ctatctttct
     8521 tcatatgaat ctgcattgtt aattaaactt tctcaagaaa aatttttgac tcgcgaccaa
     8581 gttactgaag tttgttgggg gaagttgtct accacaactt ccgacagtgc tgtggacaag
     8641 atggtttccc gtcttcgtca aaagttggtc gaccttggtt tacccgaatc gcttattact
     8701 acccgtcgcg gtcgcggctt tcagtatata aatgattaac caatttggtg acttggcgga
     8761 aaaacttgcc agtcatcgcg gtgaacttac cgataaacta cgctctcccg aagagctcaa
     8821 taagttgttt caacatctgc cactatgtcc ttatcggtta gttggtgcag ttggcaatgc
     8881 aattactcga gatcgccatt tatatagtga ccaaatccct ggttctggac caaaaaacaa
     8941 tgatttagat tttgtgttgc cgtgcgctgt ctttcccgcg gaagattgga gcactttaat
     9001 ttctcaccca gatgcttctg aggaatttga attgtgggac gatggaaata tgattgtgcc
     9061 aacaaaaaat ggaacattct gtattctaaa tccagccata tacggtgact tgtccaccga
     9121 ggagcgtcag tcaagggtag ctcgtgcctt tagccaacga attgatcctt tacacgcatc
     9181 gctacatacc gtaagtactg gcgagttaat tgtctttgta gggcaagaca tcgcacgata
     9241 cggtaaccca aaagacggtg agtacaaagg cgtctatgct gatgtatatc cgttagtgct
     9301 tggtgaagag gaaaacgatg ttgtcgctat gttcgccaga ggatacttac cgcaaaacgg
     9361 atatgtccct atattacccg acggttcaac ttttgttctt caattggatc aatctaccag
     9421 aaaaatgcca gagcttgact tgtcatatat ttccaaatca cccgctcatg ctgttcaatt
     9481 tctccgccgg cttaccaaat ccgtaattga cttatgtaat cgccgacaag ttaatgacga
     9541 actgccatac caactcgact actatcagaa catctgcgat ctagtataca caaattatgt
     9601 cttggcccat attgacagtg aagacatcgt ttcaagtaga aaaagtctca agcgtaaaat
     9661 caaaaagatg aaaaatatta taaacaaagt aaacgaagac agacaatctc catatagatt
     9721 caaaaaacta tcaccactcg tactcgatcc tatcccagtc taagtacgcc caagccaatt
     9781 aacccggtga cgattagata aattaccgga gatcatcgct tttttgagtt gaaaaatcaa
     9841 agaggttgag tattaaaact ttatctgtat cgattaaata attcgtccat attgttcgca
     9901 gattccctaa ttacttggtc aatttctgaa ccactttgcc ttgcaatctc atctatccat
     9961 acagttgact gagccgctaa atcatcaatt tctcttttgg ccctagcaaa tagagtatct
    10021 aggttttcag ttctataaaa actatcaaag ccaatatcaa ccttggtttg ggtaccttcg
    10081 gcacttttat ctcgaacttc ccaatcgcgg tagatttcat tatattttag tcttcgatca
    10141 tcatcattta aaacaaaaaa tgcttcacct acatctttaa acttctcttc tgcttccgga
    10201 tcttcttgat gccagtctgg atgagtcctc ttcgccgcct cgtgatattg acctttcaat
    10261 gcttgtttat caaaaccctc gggtccactt atacctaagg ttgcataata atttttaaaa
    10321 ttcatcccag ttttagaacg cccaagccaa ttaacccagt aacgattaga taaattgcca
    10381 ccagtactga caatagtttt ggattcatca gaatcaagat tccaaaaatt aaagacatta
    10441 gtggtgtaat atttcttatc atattgactt aattatatta ctaatttaat atccagtata
    10501 cccgaagctt taccgaaaac atgaatcaac cactcgcatc tttattacgc cccaaaacac
    10561 tcaaagattt tgtcggccaa acacatctag tgggggaggg taaacctctt cgactggcta
    10621 tcgaaaatca tcaaatattt tctatgattt tctggggtcc tcctggagtc ggcaagacca
    10681 ctcttgccaa aattatcgct cacgaatcac aggccgatct ttatgagctt tctgctgttt
    10741 cagctagtaa agacgatatt aaaaaaatta ttggcaatgg ggagcatggg ctagttaaaa
    10801 agattttatt tttagatgaa attcatcgtt tcaataaagc tcaacaagat ttcttgttac
    10861 cttatgtcga gtctggtctg ttaactctta tcggtgccac caccgaaaac cctagctttg
    10921 aggttatttc tccacttctt tcccgttgtc gcgtctttac tcttaaagaa ttgggtgaag
    10981 aagaagttag ggaaatcatc aatcgtgctt ctaaagttct aaaggtcaaa atttctaaag
    11041 ctccacgtga ttggttaatc caaatggcca acggtgatgc tcgtcaggcg attaccatga
    11101 ttgataatgc ctttacttta tatggggggc taactatcga taatctcaag caaactctcc
    11161 aaaattcttt tcttcgtttt gataaaaagg gcgaagaaca ctacaacact atttcggctt
    11221 ttatcaaaag tatgcgcgct tccaatcctg atgcggctct gtattaccta gctcgcatgg
    11281 ttgaagccgg tgaggatcca aaatttattg ctcgtcgtat ggttattttt gcttcagaag
    11341 atattggtat ggccaattcc aatgccttac ttttggccaa tcaagttttc caagcggtca
    11401 ccactattgg ttatcctgag tgtgctatta atttggctca tggggttgta tatctggcca
    11461 cttctcctaa gaatcgttct gcttacgacg gtcttcgtag tgctcagaaa gatatccaca
    11521 cttatggtaa tcttcccatc cccctcgtcc tccgcaacgc cgaaactcag ctaatgaaag
    11581 atctcgacta tggcaaaggt tatgagaaat acactaaagc agatctcttg ccagacaaat
    11641 taaagggtaa gaaatatttt aaggagataa agaaaaaata aattaataat ttgaataatt
    11701 atggaaacaa ataaagtaaa agaaatctat tcaagcttag tagctctata tcaagaagct
    11761 ccagaaaatg atggcactac attttccgaa aacggagttt gggatcatta ccataattta
    11821 attgatcaat taaatcaagc aacggggaat gactataata tatgcaaagt taacgtccaa
    11881 acacaatatc ataacgggag accttatctt tatgttggta agcgtgagta tagaggtaaa
    11941 ttaaatgatc ttatttcgag attacacaca cagttttttc cggacgagtc caatatgacc
    12001 catccacaat caataccttt tttacagcaa aacattaata acaaaaccga ggtcgagaca
    12061 aatgtatatg tgtcattaat gttgacttta aataatcaac ttcattcaat aaaagaaaaa
    12121 gtggaggacg acaatgaaaa aaactttgtc aacgctttaa ttaaaaaagt tggagatata
    12181 aaggacttta ttcagttttt aacaataact attacgactg caactcaatt tggaattagt
    12241 attgaacgag tccttgagtt attcaagatc aaataaatac catgctcaca aaaactaacg
    12301 aaacacaggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 caaatcgcca ggtggaaaca ttcgggagaa gaatggggga gttttcttga tatcaaaatt
    12421 aatgctccag aattattaaa aaaagaatta attaaattat ctcgtagaga cgccccggtg
    12481 gggcgtctca ttcacacatc taccaaagat tttggtttta tcttttttag ctctgtcacc
    12541 gacccctata ccagtttaga agccaaatac aatttaactc gccagtgttt agatattctc
    12601 gccgagtttg gttatgaagg tggtatcggc atacaaacca aatcaccttt agttttaaaa
    12661 gatatcgata ttttaaaaaa gttaaagaat gtccaagtcg gttttactct caccactctt
    12721 gacgataaag tttctcgatt tctcgaggtc tatgccccgc caacaactga acgtattaaa
    12781 gctttaaagg aattaaataa aaatggtatc aaaacttatg cctttatcgg tccgcttcta
    12841 cctcattttg taaaaagtaa acgagagata aatgagttgc tcgatgcgat ccaagaggcc
    12901 gggacgacag aagtttggtt tgaacatatt aatctaagtt ccaagattaa agagagatta
    12961 ttcgagtatt taaaagtcga agccccagaa ttaattcctg attttgaaaa ggccgataac
    13021 caggagtatc gtgaccagct gaacaaaaca atcttcgact gtcttgaagg caggaatcta
    13081 aaattaggtc taaacaaaat catctaccat cacgatttaa aaaagaaaaa atgaaactcg
    13141 cacctggtga attttcaaaa aaagtttggg agctagccct caagattcct cccggccgcg
    13201 ttgtcaccta tggctatctt gctgtctccg ctgggggcca cccaatgatg gctcaaatga
    13261 tcacccacat tttaagtaaa tcacctgatg ctgacaaagt tccttggcac cgcattgtct
    13321 atgccaatgg aaaaatttgg atggatccaa agtatgacaa aataagagct aagctttaca
    13381 aaaaagaggg gattaagctc gacaaaaaca atcgcattat tgatcttgaa aagatattcc
    13441 accacttcag ctaatcttca gcctcaggac tataatcaac ctatgagaat tttagtcgtc
    13501 gaagatgatc accgtatcgc cacctccatc aaaaaaggtc tcgagcaaga gcgaatgact
    13561 gttgacttgg cttttgatgg ccctgacggg tatgaccttg ccgagagcga agattacgac
    13621 cttctcattc ttgacctgat gttgcccggt atgactggta tggagatatg taccaaatta
    13681 aggcaaaata aggttacgac ccccatttta attttgactg ctcgtggtca aattgaagat
    13741 aaagttgaag gtcttaatcg tggtgccgac gactacctta ctaagccttt tgcttttgaa
    13801 gagttaattg ctcgtattcg agctcttgtt cgtcgaccca aaaatatcaa aagtgaaatt
    13861 ctaactattg gtgatcttag tcttaaccta agcagttttg atgtcatccg
//
LOCUS       DDWP01000036            4075 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_50015, whole genome shotgun sequence.
ACCESSION   DDWP01000036 DDWP01000000
VERSION     DDWP01000036.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_50015"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1124 a          882 c          722 g         1347 t
ORIGIN      
        1 ggctagtcac aaagttaaaa aggttggtat cttagccact cccaccacta ttaggctaaa
       61 tctatatcaa gataagcttc atcttcacgg tatcaaaact tttgttctcc catcaccaga
      121 tcaaaaaata tatgaacaaa taatccgctc ggttgtgtct ggtaatactc catcaacttc
      181 ggttgattat ttatttaaat caaccaatag ttttataaat cgtcattcgc tagacgccat
      241 tatcttaggt tgcactgagt tacctttagt ttttcctaaa gaaaaattca gcattccaat
      301 attggatagt ctcgacatat tggccgaatc gctaattaat taaaatgtta aaatcaaatc
      361 atcttatgaa ccaagaaata attgaaaaat acaatcaagc acttacctct ctgggtatag
      421 agtttactca acatcctcat cccgaattca aggaggtcgt cgactgcatg cgttaccttg
      481 gtctgccaat ttctgagggc ttttccacct tgattatgaa ggcagacgat agatatgttg
      541 ccattattcg tcgtgacgat tgtcagctca atttaaaccg tgcctgtaag gctattggtt
      601 gtgtcaaatt aactctcgct tcccctgctg agtttgaaga actaacccat cttccctctg
      661 gtgctgctca agcttacaat cccggtattc ccacttatct tgatcaaaaa ctctttgaca
      721 aagaatattt aatgggtggc tcggggtcat taagtgttac tactcgttat aaatccgccg
      781 atcttaagaa aattcccgat agctatgttg ttgatattgc cgatatcaaa gccaaacaag
      841 ttatccttac cggtgacact cctagtggca aactccacat cggtcattat gtcggtacct
      901 tagaaaatcg tgtcaaactt caacacgagt atgagacata tattttgcta gccaatgttc
      961 acgcctatgc caatgactat cgtcagtttg agaaaattaa tcgagacgtt tacgacgttt
     1021 atcttgataa tctctctgtc ggtatcgacc ctgaggtttc gaccatctat ctagaatcag
     1081 aaattcctga gaccatggag ttgtatagct attttatgac catggttact catgccaggg
     1141 ctatgcgtaa tccttcggtc aaagaagaaa taatttataa gaagcttgac gccaccatgg
     1201 cttttattgc ctatccgatt cttcaggctg ccgacatctt aggttttaaa gccaatttag
     1261 ttccggttgg cgaagatcaa tctccagtag tcgaacagac tcgagaaatc gctcgtgact
     1321 tcaacaaagc ttttggagac atttttccca tcccagaggc catgattggt cgagttgctc
     1381 gactaattgg tactgacggc caaaagaaaa tgagtaaaag tggcggcaac tctattttat
     1441 tatctgacga cgaggctact ctcaaaaaga aaattaactc ttgctacacc gacccaaacc
     1501 gtattcatgc cactgatccg ggtgaggtcg aaggcaatcc cgtttttgaa tatcaccgtg
     1561 ccttcaatcc tgataaagcc gaagttgccg atctagaagc tcgctataaa gttggacaag
     1621 taggggatcg agaagtcaaa gacaagctct ttgtggctct caataacttc ttaatgccca
     1681 ttcgtgaaaa aagaaaattc tacgaagatc gtcccgaggt cgccaaagag attctctctg
     1741 ctggtaatca acgggctcga aaaattgtta cagctactct aggcgaagtt aaggaaaaaa
     1801 tgggtttcct tggtttatta aaaaagataa aataacatgc ccctttggct aacattaacg
     1861 gtatttggca tgttctcggt tgctatcgct gacattagtc aaagggtatc tctaaggggt
     1921 cagtcaccgc ttagtagtat tactaataat tttattgttt ggaacggtat tgggtttctt
     1981 tcacttatct attttctttt gtcggggcag gctgtcccca cgattccagc cagctttctt
     2041 tatcagttaa ttcctatagc tatcctttat tttcttggtg gcagttttta ttatcagagt
     2101 ttcaagagca acagtgtttc tatttcagcg gttctggcga tgatttcgtc agttatcact
     2161 actggattag ggattatgct ttatcaagaa agtgccaatc cctacaagtt ccttggatct
     2221 ataatcgtta tttttgccat tattctagta aatttcaaaa aaggcgcaaa gctcgacaag
     2281 tataatattt atgccctttt gggaggtatc ttctacggtt ttgcttatac tctggacaag
     2341 cattttgtca tcagcacctc tcccgacttt tatcaaatgg ttttgtgctt tgctgttggt
     2401 atttctagtc ttatttttag tcccaagact atcattacgg agacaaagtt gttccaccgt
     2461 ggactaattt tctctatctt gtcctcaatt tttttctttt ttctttatca aaaattttat
     2521 tttcttgcct acaccgccgg tggtgaagtc ggtagaatcg atgtcttaaa taacaccaca
     2581 atttttatag ttatttttct cgaattcatt ttacttaaag aaaaaaacaa tctaaacaaa
     2641 aaaattatcc ctgcaatctt agctgttatt ggtgtggcta ttttagctct ggcaaagtag
     2701 tttagtcttt ttgaaaacca aagtttcttc ttgtgtgttt cgtctcagcc gctttcttcc
     2761 ttctaactgc aataggaggt aacatttctt gaactgtgga gtaagttatg acacctactg
     2821 gcgtaacaag ttcttccggt tttactctag ttgttcttgt gctcataccc aattctctgc
     2881 cagatcctct tactatatta taaccaacta aagttccttc aggtaagtct ggtccggagt
     2941 tgccatggtc tatttggtga tcagctataa ataacccacc tcttctggca aacaatgttc
     3001 ctctttttac caaaaaatct ccagctctca atacttgacc caatctccca gattccaata
     3061 cttctttttc ttttgacatt cgtccattct atcaaacact taaccttatg tcattatact
     3121 cctatcctct acgtagaagt ttattgtagg catgcatgga ctcgaaccat ggacccctcc
     3181 gacgtaacgt cggagtgctc taacaaactt ttaatttaca tgtttgttcc tctattatcc
     3241 tctcaacaat tattcgagat tttagttttt ttaatttgta ttcaacttgt ctggcgattt
     3301 caagagaatt atattcttgt ttaaatacaa ttttccaagg tatccctgtt ctcgttgatc
     3361 taacgaatcc ctggttatgc tgtgaaacta atctcctttc tggattgtcc gaactacctg
     3421 tgtaatatcc acctgtcttt tcacttttta ggatataaag atatcctttc attgtaggca
     3481 tgcatggact cgaaccatgg acccccgact tataagatcg gtgctctaac cagctgagct
     3541 acatgccctt agtaagtaag ccaggactcg aacctggacc cctccgacgt aacgtcggag
     3601 tgctctaacc agctgagcta catgcccaaa aattgttaat tcgtaattat ctaaagtagc
     3661 agaggcgaga cttgaactcg caacctcagc gttatgaatg ctgtgctcta accagttgag
     3721 ctaccctgcc ttattttaaa gttctaaact attggttttt aaaaccatgt agtccagttg
     3781 tggggtttat ccccagtccg tatcgagcgt tagctcgcat cggacagcta ccctgcctcg
     3841 taataagaat taggcactag gattatatca aatcttcact gtttattcgt tgtctttatg
     3901 tctatcacta agtgcacttc tggtaactta aacatataca cacttaatac atattatagg
     3961 tatctattgt atcaccactt tgttgtttta tcatcaacaa tttattgctt atctaacctt
     4021 aatgagtggc acaggggatt gagagcacaa aaaagccgtc aaattaatga cgacc
//
LOCUS       DDWP01000037            5801 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_19191, whole genome shotgun sequence.
ACCESSION   DDWP01000037 DDWP01000000
VERSION     DDWP01000037.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_19191"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1679 a         1202 c         1124 g         1796 t
ORIGIN      
        1 cttgaggaga ttcgtcagca agttaatagc ggtttgattg ataagcttcc ttcagatttt
       61 gttgttagtg ttaagggtgg tcaagtcagc actaataaac catctcctta ttgtttgtct
      121 ttacctgacg atcaagaaaa gactggtatt atctttgacg tcgataagtc tactcctcct
      181 gaccccagag acttagagtc aggtggtcgt taccaagaac tctgtaatcc cgcagctatt
      241 gtgggtcaag aatacattct ttttcccgac aaagattctg gttatcgtct agttaaagtc
      301 agcccggaga tgaactttac tgctaacaaa tctactatta aaaacattgc cgatcaattc
      361 tttcccattg cggtttatct cagccattgg gcttatttta ttgggccact tcttgtaacc
      421 ctctttgtct acggttcaat gttgctgttc aacttctggt atgcctttgt ggccaaattt
      481 gtcagtaaaa ttttcaagat taagacctcc ctcgatcctg agaaaaccta tgacatcacc
      541 ttgttttatt acactatcat taccgctata gactggtttg tttttggata tatcgtcaag
      601 acggttgctg gtgttgaaga tattaatact tcattcttta tgcgcaatac catcattatc
      661 actctagcta ctctctatta ccaaagttca cacccaccta aatctgaatc tttaccagaa
      721 gatactcaaa atcacaaatc tatttcgaag tagcccgtgt ttctggcaat agttcgtttg
      781 ttaatttaag aaaagtatcg aatccatcat tcaaaaatgc ctgttttttc gcctgaccag
      841 tagtaattga accttctcgc accatttggt ttattcttcg gtggacaatt atttttcctt
      901 ctctactatc acttttccac cacctgcgtc tcaattccaa aaaatcttta ggtaaattta
      961 ccataatacc ctattatacc acaaaaaatt gttacttggt cttatctttg actaaccact
     1021 ggttcctggt ccgtaataca taaattaatt atagactttt acacttctat ttcaatccct
     1081 accgggcaat gatctgaccc aaaaacctca gtcaatataa aagaatcttt taaacttttt
     1141 tttagttttt catctataaa aaaatagtcg attctccagc ccacatttct gtctctggct
     1201 ctggtaattt ggtcccacca agtataggcc actttatctg ggtgaacata tctaaaagta
     1261 tcaatccacc cttgtttttc ccacttggtt attacctctc tttctatgtc cagaaaccct
     1321 gacacttttt tattttcttt tggccttttt agatctatct cctcgtgagc cgtgttcaca
     1381 tctccacaaa atattacttt ttctcctctg tctcttaagt catttatata ttttagaaaa
     1441 tgttcgtaaa aatctagttt gtattgcaat cggcccttat ctcttttccc attggggaaa
     1501 taaacgttaa gtagggtaaa ttcatcaaac ttggttatca gtacccgacc ttcattatcc
     1561 cagtcattat ctcggtcacc cacaaccact gctcttggtt ccatcttggt ataagtccct
     1621 acacctgagt aaccagcttt ctcggcatca tttaggaaaa aatggtaacc acctggtgca
     1681 ccctctggta tttgttctat ttttgccttg acttcttgta agcaatatat atctgctttg
     1741 tcttcagaca taaatttata aagacctttt ttaacacaag ctctcattcc cgccacgttc
     1801 caagatatta ttctgatttt gttcgacata caccatttta tcagtatgca aatcggtgta
     1861 taataccgat atgagattct ttaaaaactc aaaaaaacaa ggagatatgg gtttgggtgt
     1921 cgcaatttct tattttacta gcattggaaa cactgttgga atacctctta cagatagtca
     1981 ggattatgat ttaattatcg atgatggtaa agcactatat agagttcaaa tcaagaccac
     2041 tagttataaa aggttaaatt acgaagtaaa tttgtcagta aaaggtggta accggacaag
     2101 tgtgggcaaa attaaaaaat tcgatattaa tcagtgtgac tatttatttg tagtgacaga
     2161 gtcactggac aaatacttaa taccgagcaa agcattactg gccaagcatt gtctagcatt
     2221 gggatcaaag taccaaaaat ataaagttta gtatctatat acaagatagc cccttgttct
     2281 gatagaatag ccagagttta gtctataata gacatacact ttaaaagttt gttctaacac
     2341 cggcggtagc aaagcggtta tgcgttggtc tgtaaaacca atttaggcag gttcaactcc
     2401 tgcccgccgg acaaaaagct tgaagttgca attttcttaa cacagtatca aactgttagt
     2461 aatggtttgg aaaatattta gtttcaaagt tttattgttt attagtattc taaccaacct
     2521 tgttcttatt tttcgaattc ccagtcaaaa tcaaccggta aagatagtcg aatctcctcg
     2581 gtatctacct tctattacac tgccgtttta tccagacgaa atatactcgc actctttgta
     2641 caaaacgcac ggagattata actttgaaat caccacaact aatctcgggg cggacgagtt
     2701 tatcagtgat gcttgtgata attgcgacat aactactttt gattcaaacg gccgactggt
     2761 agagcttatg atcaaccctg gaagtaaaaa gattgcttca attcgggtgt cacccaaaat
     2821 tgctggtggt gacttagtag ccagcatgca gatagagacc gtggacaaaa aatctggcaa
     2881 aactatcgag tttcctgaaa gtgagtcaaa ctactggctg gaaataatca aaaaatggta
     2941 aaataggctg tgcacctttc ttccctaact ctcgaaaact ttcgttctta ttcaaaaaaa
     3001 tcacttaatt tctctcctgg cattaactta atactaggag ataatggtac cggcaaaact
     3061 aacatccttg aatcaattta ttttttgtct tcaggcaaaa gtttccgtag ttcatcctta
     3121 tcgcagctgg tgagatggga tagtacctat acctttgtta acggtttatt gtcagacggc
     3181 actgatcttg aagttcaact tattaaagac ccaaacaaga ctactctggg gcgcaagttt
     3241 tatctcaaca aagttgttaa gactcgtccc aaatatctcg gtactttcaa gacagtcgtt
     3301 tttgaaccag aagacattcg ccttgttact ggctcaccta gtcgccgtcg tgattttctt
     3361 gattcagtgt ttgccactac tgaatggcgc tatgctgggg ctctatctca attcaaccgt
     3421 gctctcaagc atcgtaacga gcttttagac atgattcgtg atggtaaaag ctctcgccaa
     3481 gaactctttt attgggacca gtcacttata aaaaatgatg gaatcataca cgattttcgc
     3541 ctaaaatttt ttaagtcgct taatggatat ttttccaaac atgacaactc ggagataaga
     3601 actatttctt taaactatca tccttcattt ttaacccaag agaaacttga ccaaaactat
     3661 tcttacgaac ttgcttgtgg ctatactcag tcaggtgtcc atcgagatga ttttagtttt
     3721 gaaaatgaca ttttttctgc atccgacaaa aatcttgcct tttggggtag tcgcggtcaa
     3781 caaagattag cagttttagc acttagatta gggcaaataa gctatctcga ggactcttat
     3841 tcccagaaac ctatcttgct tcttgatgac atattttctg aactcgatga ccagcatcag
     3901 aacctagttg ttgaaacttg ccataactac cagacaatat taacttctgc cgacaaagat
     3961 attcaattaa aattacctac tgcacaaacg ctaaatatat aatataatac cttcactgtt
     4021 ctgcaataca gaacgatgtt ccttaacatt taaaatcact ttttttaagc cacggctatt
     4081 agtaccagga agcgaagtcc taatgttaaa tatggacatt ttctaaaatt ttttgtacta
     4141 agagtttgat cgtggctcag ggtaaacgct ggcgatgtgc ctaagacatg caagtcgaac
     4201 gatttttatg gtcttcacta aggaatgctc tattctttag tgaagatcgt gagagtagtg
     4261 gcgaacgggt gagtaatgag tacgaacgta ctctcaagcg gaacatagcc caccgaaagg
     4321 tggggtaatt ttccatagtc tcgcaagagt aaagatgcaa atcacttgag aagcggcgta
     4381 cttgccacca gctagttggt agggtaacgg cctaccaagg ctataacggc taacggctct
     4441 tagcggagag tccgtcacaa tgggactgag acacggccca tacttctacg gaaggcagca
     4501 gttaggaatc ttgcgcaatg gacgaaagtc tgacgcagcg acatcgcgtg aaggatgaag
     4561 gccctcgggt cgtaaacttc ttttattttc tcatgaagag cttttgaata agcaactact
     4621 aactacgtgc cagcagtcgc ggtaatacgt aggttgcaag cgttacccgg ttttattggg
     4681 cgtaaagcgt ctgtaggtgg tttgataagt cttgtttcaa agaccccagc ttaactgggg
     4741 gaaggggcaa gatactgtca gactagagtt atatcggggt tactggaatt tttagtgtag
     4801 gggtaaaatc cgtggatact aaaaagaaca ccaagcgcga aggcgggtaa ccaggtatat
     4861 actgacactc agagacgaaa gctagggtag ccaatcagat tagagacctg agtagtccta
     4921 gccgtaaact atgtatgtta attttctgtc cctttgggat gggagatgta agctaacgcg
     4981 ttaaacatac cgcctgggga gtatagccgc aaggccgaaa ctcaaacgaa ttggcgggga
     5041 ggcgcacaac cagtggagca tgtggtttaa ttcgatacga agcgatgaac cttacctggg
     5101 cttgaaatgt acctgcaaac ctccagaaac ggaggaatcc ttagagggtg gtacacagct
     5161 gttgcatggc tgtcgtcagc tcgtgacgtg agttgttctc ttaagtgagg taacgagcgc
     5221 aacccccatc cggtgttatt gtgtcaccgg agactgccta gcattatttt gttaggagga
     5281 aggaggggac gacgtcaagt catcatgatg cttatgtcca gggctacaca catcctacaa
     5341 tggagagtac aatgggtcgc gaaaccgcga ggtggagcca atcccacaaa actttcctca
     5401 gttgggattg caggctgaaa ctcgcctgca tgaacccgga attggtagta atcgcgcatc
     5461 agcagcgcgc ggtgaatacg ttctcgcctc ttgcacacac tgcccgtcaa atcagcaaag
     5521 ttgggggcac ccgaagcttg gcctttggcc gaatcaaggt gaattcagcg atgagggtta
     5581 agtcgtaaca aggtatctgt actggaaggt gcagatggat cacctccttt gaggttttct
     5641 ttgaagttat caatctaatt tattagaaag aagcttcatt gtatactcaa aacctttctg
     5701 gtactaataa tcttggctta aaaaagtgat ttaaatgtaa aattatcctc ccattcgtgg
     5761 ggggattttt tttggtatat aattgcgcaa tggataatat t
//
LOCUS       DDWP01000038            4765 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_33007, whole genome shotgun sequence.
ACCESSION   DDWP01000038 DDWP01000000
VERSION     DDWP01000038.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_33007"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1389 a         1319 c          589 g         1468 t
ORIGIN      
        1 gcacttatat tgtcagaata acttttgaat tttaagtttt gaattttaag ttttcaaaat
       61 ttataagata taaattttga atatgtcacc ttatcgctcg ccagtataca tcaagatttt
      121 ccagagctac cccaactccc ttaatcacac acaattgtgg ctcagacgcc acatgagccg
      181 agactccaat ttcccgggtc accaaaatat taaagtttct caaattcgat gtccctcccg
      241 acagtactat ccctctatca acaatatccg ccaccagctc cggaggagtc gactccaatg
      301 tgtccctaat cgccataatc attccatcta aagtcggtct tattgcctca taaatttgat
      361 ccgagtctat cgataccgcc ttaggcaaac catagataga atccctacca ttaacttcca
      421 tcacctctgg tctctttagt tttatggcac taccaatttt tatctttatc gattccgctg
      481 tctgttctcc cacaatcaaa ttatgttttt tcttcaaaaa ttctaatatc gcttgatcta
      541 tctttgtccc tcctaagcgt actgtcttat gtgtcaccac tccaccaaga gaaatcaccg
      601 ctgcctctgc cgccccacca ccaatatcta caatcatatt tccataaggt tcagatacag
      661 gaatcttagc cccgatagct gctgccaaag gttcatcgat taaatagact tttcgtgctc
      721 cagctgataa agctgcgtcc ataactgccc tctgttcaac ttgagttact ccagccggga
      781 cacagatcat tacctctgga ccaataaacc aagctctacc catcacctgt ctcaaaaaat
      841 acttcatcat tgcctcagtt acttcataat cagctatcac tccttcttcc attggtcgaa
      901 taacctcaat atattctggt gctttaccca acattttttt ggcttcatct ccaactgcca
      961 ctacctttct atcatctgcc cctattgcca ctaccgtcgg ctcatttaac acaatcccca
     1021 ctccctcctc ccagaccaca ctattggccg tccccaaatc tatacccaat ctcttaaaaa
     1081 gtttcatcac accattctat caattttaat tcttaattat taattgccag ttgatagttg
     1141 gtataatact ctcatgtctc cacacaaaga taaaaaaagc cttattgtca tactttccgt
     1201 attttcactt attatctttg gtacttattt catcactcta ctcgctcgtg gctaccgacc
     1261 acacataagt cagaccggaa tcagcctcaa atccagcggt ttactttccg ccacctccaa
     1321 gccaaaaggt gccggggtct accttgacga caaacttatt acagctaccg acgacactat
     1381 taacctacct ccagatcaat atttccttaa gatagttaaa gatggctatt tctcttggca
     1441 aaaaaacatt aacatcaaaa aagaaactgt cttccagtca gatgctcaac tgttccgatc
     1501 ttctcctgac cttcgaccta tcactcaaac cggtgctatc aaccctaccg ttagccctga
     1561 ctatagccga gtcatctatt ctgttgcttc agcttctgct gccgctgaca acggcttgta
     1621 tcttatcgag ctggcctcct cacccctagc ccttaccaaa aatactcccc gtcaaatatc
     1681 tcccaactat ccaagtatag actggtctac tgcaactttt actttctccc cggattctcg
     1741 ccaagtctta gcctcattca ccacctcagg cgctaattat cttttaagcc tcgattctcc
     1801 catcaatttt tcgtcactag tagatgtcac ctataaatta cctctaatca aagatgagtg
     1861 gaaaataaaa gaaaaagatc ttatagatgc caaaattgct caactccccc tctctatcca
     1921 accagctgtt tctaccgaat ctacaagaca tatctccata tctactactg acgataaaat
     1981 cctttatctc tctgctagcg attcagctat ccctgacaag atattgtccc cgcctccagc
     2041 tcaaagtacc caacctcaag ctcgccaagt caagtcagga aactattatg tttacgatgt
     2101 taaagacgat accaactttc ttgttggccc caagtcctcg gttcaaaacc cattttggct
     2161 ctacggctca aataatatag tttatgttga gaatggatca attattggta tcgactatga
     2221 tgccaccaac aaactaacta tttttaacgg acaagtagag accaacaatg tcttcccctg
     2281 ggcagatggt actaaaataa ttgttcttac ctcagcctac tccggatcaa cccaaaacct
     2341 ctacgccatc actattcgct agatattact acttgcaagt attttcttct atcgaaacta
     2401 gttgagttat gacttcattc cattgttcac cattaagttc cataccatct atttgacttt
     2461 gcaaatcagt aatcatcaat tgtgattctt cagtagtaat acaatctctg actacgactg
     2521 tctcctcacc caaaaccgcc atcgattcaa tttcattata cggtgcttct aagacaggct
     2581 cttcattcac tacttccaaa tcaatttctg gcaacaccac ttgggtatca acatctccca
     2641 aaacctgaga ctcacccacc atctgaccaa cctctacaaa cctcaaagga gttaacacca
     2701 ttatggccac cacgaaactt acaatcaaag tgcgagtgtt tctatttatt tcattcataa
     2761 tatcagtcta gatttttttc accgatagtc aagcatttat ttatttaccc ccaagcaccc
     2821 ccataaaata ttcaataaac tgcctggcag aaaaagccca ttcttgccaa aatcacctgc
     2881 tctgttttac caaaaataca tcaaatacta tttttgatta tcttaataca tcaaaactta
     2941 attgactaaa acctagataa gcattatata attcacctag ctttgcatga acggggattg
     3001 gcgcaattgg ctagcgcgca cggttcggaa ccgtgaggtt gctggttcga atccagtatt
     3061 cccgacctta gttcgttgtg gctttagcaa aagcaactcg aattcctgtc cctatagttc
     3121 aatggataga acggaggttt cctaaacctt aaatccaggt tcgattcctg gtggggatac
     3181 actaatcaga caataaccgc acaaatcaaa cccaacaaga cactttctcc gctataaatt
     3241 aacacttatc cgcactttct ccgctatatt tttcctcatt tttaccctca ctattctccg
     3301 catatattct cacaactgat tacttgctaa gtgcaacaaa gaatactcaa aatatatctt
     3361 attcaccgca aacagaccta aacagactac tttttcactt catcactctc tattttttca
     3421 taaaagccac tccacccaag ctgtaacttt ttctagaaaa aaggccattt tatgtctata
     3481 tcaattaaaa tcatctacct ccaaaatgtt tgcctataca aaacagcttt agttacctat
     3541 cgacaaaatt ctctacaaaa tacctaaaat aagacagagc accaataaca caaaaaacat
     3601 tgaaaaaacc gataaaaaca catcagtgca atacgtagca gtcaagagat atctttgcca
     3661 aaacagttat gacaatcaaa aatatgtact tttcaaccta aaaaaattac ctcgatatta
     3721 ccaatcttct tatcttatta gctacaaaaa tactctttgc cagttctctc actgcctcta
     3781 aggtaaccgt ttccatcttc tttagctctt cttctgggtt tatcaactca ccttctaaca
     3841 gtaatttctc acctacatag ccagaccaga attcactatc atccagattt aatgtcactt
     3901 tacctcttag atacgtttta gttctgtcaa tctcctcgtt tgatacagtt aatgcgaagt
     3961 taatcatttc cttctcaacc acagtcatag cctcaagtag gttctcttgt tgtacccctg
     4021 cctgtactgc ccaaaaacca gtctccctcc atgactcacc agccgaaaag acatagtaag
     4081 cccagccccg tttagtccta atttcctcaa aaagccgtga agaggcattt cccgtcatta
     4141 ttatgtttaa caaccttaaa gcatgttttc ttttatcaag ccatccaaat gtaggtactg
     4201 ccataacaaa gtgcccttgc tcaacctctc tcttcatttc ttttaaaata ccttcactta
     4261 atttaatctc cacttctggc accacacctc cctctgatac cccaccctca aacaaatcct
     4321 tcaccaagtc gatatcttca ggtttaacat caccagccaa ggtcaccacc atttcttgag
     4381 tatttaaata tttatttctg aactcaacaa tcttctctct atcgaccttt actatatcct
     4441 cttcttctcc tgagatattc caacagccaa ttttagatcc tccatacata aacttaacca
     4501 tctctgagcc cattcccatc attggattat ctttgtacat cctgatttct tctgttatca
     4561 cccctcgttc cttcataact tcctcctcag gcattgtact ctcaaacaaa atatctgcca
     4621 tcatccccac tccccaagct aaattatctc gaaccgtagt tatactatat gaagtgttct
     4681 cataaccggt ctcggcatta taacctaccc ctttaccatc catctctcga aatatatcca
     4741 tcgctcctgg cctttttcta gtccc
//
LOCUS       DDWP01000039            7263 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_43463, whole genome shotgun sequence.
ACCESSION   DDWP01000039 DDWP01000000
VERSION     DDWP01000039.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_43463"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2356 a         1264 c         1576 g         2067 t
ORIGIN      
        1 gaagaaaaga aaacggagta tattatgccg ccaaacacac ccaagaaaat taatagggaa
       61 atgaagaata gatacttgaa aatattgtta gaaacaggct ttggagaaaa atcgttaaga
      121 gaatcaatcg acactgaaat ggtactctca gcggtcggtg gtgatgtaga ggggggtaaa
      181 ttgagtaatg gctgcgggtc aataattggg gtttcctgac gggcacttgg agcaactttg
      241 tccaaaacat cttcggagct agaggctaat tcttttgaat aacggtcaag tatgtcttgg
      301 tactgttgag agagatcttg tttcttttca tccataataa ataatcgcac cttcgctatt
      361 gactgtcaaa tgcaggattg ataaatttat ttatgcttat gctgatgttt aggttaatgg
      421 tactaggtat agtgtttttg gtacaactgg ttaacgcaga taaaataatg gcaagtggct
      481 ttaatttaaa atcgataggt tcaatccaaa ctaacagctt ggtttctcac tggtggtata
      541 catcggcaaa cccaagactg acaggtgaag ccttaccaag cgtagcggta gatgtctcaa
      601 tagatggcga aatggaaaca ataccgacaa gctctgacgg tgaatggcaa tatcaaccaa
      661 caaaattaac cgaaggagag catgaggtaa ttttgaaaag tggcgagtct attattcaat
      721 ttaaattgac gattgggacg gcttcagttg attgggatgc tgtaaacaaa ggaccggcag
      781 actccctacc agtggcggga tcggtaacaa caacttggat gctattaact agtagtttga
      841 tggtagtagt attgggtggt aaaatgcttc ttcatgcaaa gagataaaca ggtatatata
      901 aaaacatttg gttgtgcaca aaacgtagct gattcggaaa gaataaaggc ctattattgg
      961 gaaaaaggtt ttaaagaaac agaaaaatgg cgagaggcag atgaagtggt aataaacaca
     1021 tgtattgtaa gggagtcggc agaaaacagg gcttgggggc tgattgataa tatagtaaaa
     1081 agtaaacttg gacaaaaaat aattgtgact ggttgcgtgg tcggggccta cggccgaaag
     1141 gaaataaaag gtgttgatga atgggcaaaa atagacaagt ttctagtgtg ggaaccaata
     1201 agagcaaaaa agaatgtggc attaatatcg attagtaccg gatgtaacaa tcactgttct
     1261 ttttgtattg ttccaaaagc taggggtaaa gaggtatcaa ggacaatgtc aactatcttg
     1321 gcagaagtag acagggcgat tgactcgggc tttaaagaag ttgtgttaat aggacagaat
     1381 gttaatagct acgggtctga ttttgaaaaa gaatcgtggg tgcatatggg caagaagagg
     1441 attgtgagta gattccctga actactagaa atggtcgcca aaagaaaact agataaagtg
     1501 tcatttgtaa gcagtaatcc atgggacttc tcagatgagt taatcgagac tatagctaaa
     1561 tatccaaata ttgatagatt gattcacctc ccctttcagt cgggcaataa tgaaatattg
     1621 aaaaagatga acagagccta tactaaagaa gaatatttgg agctagttaa taagattaaa
     1681 tccaaggtgc caggagtaag atttagcaca gacataataa ttggcttctc cggggaaact
     1741 gaagagatgt ttgaagacac agtcgatgtt tgtaaaaagg ttggttttga aattgcttat
     1801 ataaataaat attcgccaag aactgggaca gtgtcagcta aaatgatggc aaatgatgtg
     1861 tcaataaaag agaaaaaaag acgatgggtg gtattagatg agctggtgaa cagtaagata
     1921 acgatgggcg agaaaaatgc caaatgaatg ttctgaactt ttgaccaaag atttgtcagt
     1981 gcccaaacta ttgtagactt ctaggaagtt tttgatttca atcgacatct caaaatattt
     2041 ttcaacatct ttgtctaaaa taggctgaga aatatgttgg cagtcaaggg cgagaaatat
     2101 gtgacaccaa tgattggttt cactaggaag atcataatat gtccctaacg agattaattt
     2161 ttctgaccta taaccagttt cttcaaacag ttctcgactg gcagtattag tatctagctc
     2221 tttttcttcg acaccaccag cacaattttc ataggtaaat tcctgaatag tatatcgaaa
     2281 ttgacggacg agaataatgt ttttgtctgt gtctagggcg acaatgatga ctccgccacg
     2341 acttttagtg taagcccagt caaatgtgac accgtctggc atttgaagag tatcaacaca
     2401 aaatgcttta ccccatttat tatatagaac ttgctggttt aaaagttttg cttgtctcat
     2461 cttaaatatt tagcaatatt aatattgtgt tcatccaagg tcttgccgta gtgcatctgg
     2521 ttgtcattac cggtaatgta gtaaaggtaa tcactttcta ttgggtgcaa agcggcataa
     2581 atagaatcat aaccagggtt gcaaataggg ccaggcggca agccggcatt aaggtaagta
     2641 ttatatggag aaacgatttt tagatcggct ttactgacag gctcccagaa agatttggta
     2701 ttgaggcgac tatcgcgagc atactgagca gtggcgtcaa gttgaagagg catcccaata
     2761 ttgagacggt taataattat cccagaaact tgccttttag aaacaagtgt gcgggcttca
     2821 cgctcaatta gtgaagctaa ggtaataatc tgggcgtcgg tcatactagt gttagtggca
     2881 ccggcttttg cttgggtaaa cttgacaaca aagtttttat taatgacaga aaaaatattt
     2941 tcaaccgagt agtcctgagg aataaggtag ctgtcgggaa acagataacc ttcgtaagaa
     3001 gagtcaaacc ttgggctaat ttgttctatt ctctgtccac caattatttt cagccagtaa
     3061 tcgtgactac caccagaaga taatttggca acgatatcgc cgttggagag gttggaagac
     3121 aacttaaaca aaccggactg caatttagaa gcaaggccta gtcgataagc atgaaataaa
     3181 aaaacatatt ggttgcgaac aaaaccacct gactctaaac gctgactgat taatttaata
     3241 ccgtcacctt ggttaactac aaagttctta actgagctat cagaagaaac cggatgggag
     3301 ttgtaataaa caaagccagt ggtaagcgta agcaacaaca agattgatag taaaagaaaa
     3361 gcgacaatct tcattagact tcatcgaaca tggctcggcg ataggtatct ttcttagtat
     3421 tgtggacaaa aatacgacca caacgacaag taatagttat ctgtccacga atatcttctt
     3481 ttttaccttt cagaagtgat tccccacaac caacacaacg cttctgtgat aacaaccatg
     3541 cctgcatcgg aaatattaga tttttaaaca taagccatta attaataaca cgatgaagga
     3601 taactgctgc tgagacagag tcaatggatt tactcctgtt tttacggctt ttatggttgt
     3661 ctgccattaa cctgtctgcc tctatggtgg aagctgcttc atcaacagtt tctactggta
     3721 attttatcac aaccgacatc tccttaataa acttttgggt taactttgcc actctaccat
     3781 agctgatacc tacgtaaatt ctttctattt tatgggaaat aattatctct ttaatatgat
     3841 caaaaacatg attgtcgtta acaattggtg gcagagggca aatgatgtca ttgacagaat
     3901 aagccaaacc tatagttttt gtgccgtaat ctattgataa acaattcata agatatattt
     3961 taattatcga caatcctggc cttgactact aaccggcgga gatacgtccc tggtggacta
     4021 caagtgatca aattcagata acgaccatca tagcgttgct ccaggacgga taagttgtct
     4081 gggctgacct catacatctc ttcgactaaa tatttgtatt ggatattatc aaaatcaata
     4141 agtatttcat ctccagtttt taaacgatat agtgttgaaa atatggtcag ataagacttt
     4201 ggattaaaga actgtggcaa aacagagtgg ccaaaaataa ctccgttacc aagctgacca
     4261 ggcaaggcgg tctgtggata ctgaataaga ctcttcttga gatccatgct gccgatggta
     4321 acgacagcat cgttaacttt caagttggga attgatattc gatatgtcat ctcggaagag
     4381 ttggagagca aagaacctgg ctcaatgcca gaatttgcct cagagacaaa ccaactattc
     4441 aattgagtgt agtcggaatt ggcaacctcc cccagaaccc ctccttgttg attatagaat
     4501 ttagcagaaa gagggttgac tatctgacca aatttagtgg catattctaa ttggaactga
     4561 acaattggta atacagcaga tgagaagaga aaaagcccaa aagaaaagag tcctccggca
     4621 ataagtttcc tctggaaaac tgataatttg aatttaggtg aaggcgacga ggacttacgg
     4681 ggctgacttg attttagata gatataaccc acagtctatt taacctcaat tttaatgttt
     4741 tgcaagcgaa aaggtagtgg gttaatatta gcaaggaaat taaagttggt ctcaatgttg
     4801 taatcatata ctcgagggta agacttggta atcaagccta tggcttttgt tttgtttttc
     4861 ccagatattt gtttgataag tccagaagtg tcaatacgag gaatggactt gccactaatg
     4921 acaacctgac tagtaatacg atcgactgtt ttactagggt tattaaggtt gatttcaaaa
     4981 tcatccaaac tgtacttgga atcaagaaag ccctcttcgc catctagaaa agaagacagt
     5041 actctagatt tctgatcagg actaagataa aagatataaa tagagttatt agaagtgaca
     5101 gtcaaatctt cggacagttc accaacttct cggttgtaat caagacgact attcttaatt
     5161 tgagtagcgt ctttaataat gcctggagta ttggtaactt cctgattaag tctttgttct
     5221 actaggtcat cgatcttgga actaatttgt tgttcaagat tagccttgtc aactgcacta
     5281 acagctcgag cttcattttt ggtaccaccc accaaaccct gtgagacttt ggctactaaa
     5341 atactctcgg ggaagtcttt aaaaactaat ttagaatctt tacccagatt atattcagga
     5401 ccgatatcag ctgctgatac cattactttt gtttgcccta aattgataac tcccaaatca
     5461 agattggcgc tacttgaggc tatttgtgct gaggtagtca attcataacg gcgaccagaa
     5521 gtatctgtca gaatagtccc cttacttagt ggctggacct tgtcttgttt attgtagatt
     5581 acgacttcac cagtagactt atctccaaca actttagtac cagtagcggc agaactaaga
     5641 gaggtagaaa cttcgacaat tttcctgttt atagggataa ttcctttaac cgaatccact
     5701 tccttagctt ctgtatcgaa agtggctttc aaacttttag agaaactgta gggagtgatg
     5761 tgaatggtaa tagtcgcttt tgaaaagaaa aatggaatca aaattagaag cggagaaagt
     5821 actagtgggt atagtaaata cttaactgac aaaaatttag ggctgaaggt gggggctcta
     5881 atcttagaaa agttgaattt tttctctggt ttttcaggga cgtttagtgg agcatccgag
     5941 ctgacttggt cataatctag gataattggg gtagtagtat catgactaaa accgagctca
     6001 gatgggtcaa cctcgagcag acttgaatct tcggataagt cttcaagaac aacttcctga
     6061 gttgtctcaa ggttatcgtc aataatttcc tcgacaggag gaggactgtc gatgactgac
     6121 ccagacactt ctggtctaaa ctgagagcaa atagttttca gatagatact ggtagttttt
     6181 gatagatcat agaacttgat ctcgggaaag tgaagaaaag tctcaatatt ttttttacca
     6241 gtccaagcaa agttgcggat tgagtctaaa accgagtcag acacgtcacc agtgacgata
     6301 atttgaggtg gtaaggctga ctctgaattg aagcccagca acatctcttc aagcatggcg
     6361 gggttaaatt ggtcttgata cgtcctaact gaccttttga taatcttgcc aagataggaa
     6421 agcgacacag caacttcatt gtcaaaaaaa tttgcaataa caaaactagc cggaaaacca
     6481 tctgactgat tagcttcttc tactaaagct tcatcattag agatgaagcc catcggcttt
     6541 aatttgagac tgtttaagac tcgaccaagt gtagattttt tctgagggat aatagcacca
     6601 tcggaatcaa cccaaaacgg ggggataatt agagcaactt cattcggctc ttgagaatca
     6661 ggcaaattga tagatgacgc caactgagaa agagactgat caaatgagat aattagacca
     6721 tcgtcatcca agtaggaggc tgtcgaccca agagcactga tctgataatt actgccagac
     6781 aaactgacta aagagacagt gactgatttg ttattgatgt ccgcaagcca gaacgagttt
     6841 cgatccatta tataaggata ctatactacg tcaaaactgc atacaaacgg cgtttaccag
     6901 cagaggcaga ttcttctttg gtaatggtaa attttccgag aacactagtg tttttgacgt
     6961 gagggccagt gcaaacttct ttggaaaaat cgccaacagt atacatggta acaatatctg
     7021 ggtacttcgc accgaaaaaa gctaaggccc cttgtttctg ggcttcgacg aaaggcagag
     7081 tgtcgcaact aactggatgg tctttaatta tctgttcgtt aactagatct tcgattgatt
     7141 tgatttgttc tgaagtaagt ttatcggggt gagaaaaatc aaaacggaga cgttcactgg
     7201 tgatatttga accagattgc tggacgtgtt cacccaaaat tctacgcaaa gatgcgtgta
     7261 ata
//
LOCUS       DDWP01000040            5132 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_28947, whole genome shotgun sequence.
ACCESSION   DDWP01000040 DDWP01000000
VERSION     DDWP01000040.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_28947"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1790 a          900 c         1121 g         1321 t
ORIGIN      
        1 agccaaattt aagagaatag agattgtcga ggtttatctg gtcgacaaaa cctttaactc
       61 tggaggcacg aagaccagtg gtgggatcag ttacttggaa aaatggtttg aagggaaaga
      121 acatgataaa acgagaggta aaacccccga cataagtaaa gaattttctt ttcatacccc
      181 aagactcagg gacggatcca ccaactatct tacgggagcc aataacaaaa tcgtaaccgt
      241 tatcaatttc agtcataagc ttgggaatat ccgctggagg atgttggaag tcacaatcaa
      301 actcaaccag gacatcagct ttgagtgtgt ctatggcata atgaaaacct ttgacgatgg
      361 cattacccat accttttttc tcaggattga cgagaagatg aagatcggga tactttccct
      421 gaaggtcttt gacaattttg taagtaccat cgggggaagt gtcgtcgaca attaagacgg
      481 aacagttcca gtttttaata gtagggataa tgtcgttgaa taatgtagcc atcatcgggg
      541 tgacggaatc tgcctcattg taggtagcaa tgacaatcac tacgtgttgt ttggttggtt
      601 tcatagaaag agtataacgc taaaaatttg ttttgtggta taaaacatcc atgtctgagg
      661 aatttgatat ggatgattat aggaaaggtg tgttggacga gattgtttgc gaaacccgtg
      721 gcaacaaaca taaatctctg gagggactcg gggtggttga gtttttaagt aggtcgaatg
      781 gcaaaaacag atcaagacgt ccaactagag aggtgagctt gttggtgtat ggcagcaatg
      841 cttttcagat aaaagataac gagattaagc caagaaaaag aggcgatatc catccagata
      901 gcttggccca agaggccttg gaatttttgc cggctgatga aagaactgta gataatcttt
      961 tgtcttatgc gagaagaaag gtgaaataaa tactacggtt tcaaactatg ttactatcac
     1021 atcagactgt aacattataa attaccctta agaaagtgcc tggagaaaaa aatctattca
     1081 aaagaatgaa gattgacttt aacgctgaca gtacagttgt gggtacatgt aaattgagag
     1141 gaagagaaag ggatgacgga gctattgggt tttccgaaat acaagaaaac gttgaacaaa
     1201 gaatgaaagt ggcaataaga cacaagatgg gatgtcaaaa ctgtggaatg aacaagggca
     1261 aggaaactat taaaccggac atccgcgtac gaatgacttt tcaaaatgaa gaaatggtaa
     1321 tgactctaat ctgtacaggc agaaaggaaa gaatggcgaa tgcaaactgc tatgctacat
     1381 ttccacccaa agaacctgtc cagttcatag taaggccgga ggatgaaata ccgttctaat
     1441 tacaaaacag tgtgtttttc ggtataatat agagacatga aaattgatgt aataaccttg
     1501 ttcccgaaaa tgtttgagtc cccttttgcg gaagcaatga tgaagcgggc gattgataaa
     1561 aaaatactag aactaaatac tcacgatatg cgccaatggg catggaataa ctacggagca
     1621 gtagatgaca aaccgtttgg tggtgatatt ggaatgttga ttagagtgga tgttgtcgac
     1681 aaagccctac gggctttaaa ttcaaaaccc aaaactcaaa actcaaaaat tattttgaca
     1741 agtgcgaggg ggaagaaatt tgatcaggcc aaggcggaag aatggagcaa gctagataat
     1801 ttgattttta tctgtggtag atatgaggga gtagaccaga gagtggctga ttatatggtg
     1861 gatgaggaaa tatcgatagg tgactatgta ttaactggtg gtgagttacc tagtatgatt
     1921 atgattgatg cggtaactag attattacct ggagtattgg gaaaagatga atcctccaaa
     1981 gttgaaagtt tttctggaga gaaacgtcat atcgaacatc ctcaatatac tagaccacaa
     2041 gaatatgaag gacataaggt ccccgatgag ctacttagtg gaaatccaaa aactatagca
     2101 gagtggaagt caaaacaatt actccaaata aaataaggat caaatattca actacaatag
     2161 atgtctcaag atcaccacgt cggtagtgat gaagaatact ccaaagaaaa taagcaccag
     2221 cggctccgta gacgacgttt ctctgataat gggggtcata actaaagaag aaaaacaaaa
     2281 tagcagaaat caacaacgag acggcaagaa agacgtactc aaaaggtttt gactctattt
     2341 ctttgaagat actcattaga aaatcctccc ctgctggttg gccagcaata atataaatat
     2401 aatagctaga aaaccaacat gtccagcagc tgaaccagct agtcgatgag tcttgttctt
     2461 caaagtaaag taaccatcaa tggctagagc actgataatg atgacaaaca tcactagacc
     2521 aacaaactga ctaatctgga gcgggcttat taatggagca gagacgggcg ctgatgatgc
     2581 ttcagaaagg atatcaactg aggctgatgg tggggatgag taagccacca cgccattaag
     2641 aggtgaacca aaatgttgaa caactagagt ggtcttgacg ccattgagaa ccccattaac
     2701 tacccctata cctatctctt gatattttgg gtttaggata ttttcccggt gagtgggtga
     2761 attcatccat gctcgaacca tagaatctgt atcataaaaa tctttggcta agttttcccc
     2821 agcaacagaa tactcataac catgttgcct gagaaaatcc caaggtgttt taccttgtgg
     2881 tgaattgtga gcccagtaat cgttggcaaa catatcctga gctttctcta tggctgaatc
     2941 tgaaagaaca gaattatatt ggagtggttc ttgacctaat ttgagacgtt cttgattagt
     3001 taaatcaatg actttctgga cagttatttc agaagaataa ccgagaactc ccggcttaat
     3061 aatagtgagt gatttaacta acgattgatt tagaagataa atcgaaataa agagggcaat
     3121 gactgatggt ctgatgagat aggcacgatg attgttactt gtatgaggaa caaaaagatg
     3181 ccaaatttta ttcacaattc agtgtagctt atttacgctt gtttttaaag acaaaaggca
     3241 ctaggtatga caaaatacta ccaatccaaa agtattgaca gagcaatgga acgataggac
     3301 aacatgaaga acggagcaaa taccagtcta caagaaaaca caaaacacaa aacacaaaac
     3361 taaaaactgg attaaaaaag aataaattaa ttattaaagc caaaacaaat aaaatgggca
     3421 accaaaacct agtacaacta cttgactccc ccataattga tcccaagtca accgattcag
     3481 aaggaacttc tgtctgctca cccaaaacac ctggctggaa accttcggga atattttctc
     3541 caggagcatt agcttcgtta atacagacga cgttggaggc aggaccaggt gcacaatcat
     3601 taacggcttt aacgtaaaaa caataacgag ctccgggagt tatcccacga actagatact
     3661 gattatcttt gcccacattg ggagcaccat aaagaaagct atctctatcg ggaccaaaag
     3721 aaattagata atgagtatag gcttgagaaa ctccattcca gctcaataaa acagaaccgt
     3781 caggattttc aactgcttga tagagagatg gagcgccagg agctggttga ctacaaacac
     3841 tggcaccagc aacactacct ccactggaac ttactgatgg cgaggctgac tcatcttggc
     3901 aagaaaagtt aaggtctaaa tcaaaaacag aagaaatgtt ttgatattcg ttaccggcgt
     3961 tgacatcaaa ctgagaactc caaatgtaaa cagcagtact gcctgaagga atggtgccta
     4021 gactatagtt tggttgaaga taaaattgtg aaattgtccc agaatattgg cttgaggcgt
     4081 tactggtaat gtccagagac attttatcgg ataataaatc gacaggattg gatttaattg
     4141 ctgtggtggt aaattggcat tcactagtgc gttcattgcg aacagtaacg gattggctaa
     4201 cttgatagcc aggaaaaatg ttcgtctcga aaaataatgg tgaactagtg tctttggaac
     4261 aagaagaaga attacaagta attagctggt cgttaccggc aaaaacacta ttggtagata
     4321 atagagcaaa aataataaaa aatacctttt tcattagtaa ttaaaatatt tagtaacacc
     4381 atcgatagta acagaaatag ttgaaccaat agaaacagcg tggggaagac atactccgct
     4441 actacaagta ccaagataga aatcacggtc agatccacca tctacgatag atatagtgcc
     4501 atatattccc tgttgattga gttgatcgtt atagattatc tcgtaatcaa cactgtcaga
     4561 taaattgaaa acagataagg taactttgtt gactgatgat cgagagacga ggttaaaatc
     4621 gagactatca ccatctttag cccatgtacc aataccgaat ggggttcttc gccagatatc
     4681 accagggcta atttgaggag aagtgtcaac atcactatca ccatcgttaa gaatgtaagt
     4741 ttcggtataa ccaacagaga tttggacaga ctgatgagaa ggcaggactg tagacccaac
     4801 agctatgtta ttaactttat aactggaaat atctatatct tcagtagtat tgttgtagag
     4861 ttctataaag ccactagagt caatcttatt gataaccact ggaggtaaga cgacgacagc
     4921 aactgattcg gtggactcaa cgttggtggc aatatcatca ttttgatact caacaacatt
     4981 ttctccaaca gttaaatcag aaggaccttg tggtaatagt attccagaac cagtatcgtg
     5041 ggtgtcaaaa tcaagagcaa gtgtctcacc taagcgtagg tccaggctat taactttgat
     5101 atcgtcaacg taagtccaac tcttgagagt gt
//
LOCUS       DDWP01000041            7339 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_37720, whole genome shotgun sequence.
ACCESSION   DDWP01000041 DDWP01000000
VERSION     DDWP01000041.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_37720"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    2401..2425
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5599..5636
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2021 a         1541 c         1258 g         2456 t
ORIGIN      
        1 tgctccacct ggtttttctt ttccaccaga attatctctt gcaggtagta ctgtttttgc
       61 atcactagct gatggtcttc cttttttttc tgacatgggt cgattctatc agattgttta
      121 tttttttccc atcagtctag cagcgatgta tgtggttatt ggcacggcta ttatcaaccc
      181 catacttcca attaacgtcc tgacaatttc ttcagctatc atttcatgat tgattatttt
      241 tccaaatggt tggggattat ccacaaacat taacagtaat ggcaacgaca cgcccgcata
      301 agccaagact aaagtattca ccattgaagt tatatgatct ttacccacgt tcatcgccct
      361 ggtatatagt tcgcttgcct tagttatttt ttctgtctta gctagctcgt taactattcc
      421 tgcttgagat atggtgatat catccagcac acccagtacg ccgataacta ttccagccaa
      481 tagcaaacct ttcatattaa gcatgccctc tcggtttatc gacaacattc ccgcatcttc
      541 tgacgacaac cctgtcagat aagtcaggtt tataaaaata aacgctaata agctagttat
      601 tactagggct atcaacgatc cagccacagc tgatgctgtt tttttactta tgccatgaga
      661 cataaaaaaa gataccggaa taataatcat tgacgctacc gaagcgatca gtattggatt
      721 ttcccccgaa gatattgccg gcagtacgaa aataaataat attacgaaag tgaacatcat
      781 tcccgcaagt gagcttaccc ctttccacct agccactgtc accgttatgg ctataaacat
      841 tattaggagt aaagccaaac catctcttct ttcaaagtct gtaattatcc agaactcatt
      901 tccctcagcg tcaccgctca tttccaataa tactctatct ccacttttgt atttaatatt
      961 attggctaat attccactat tattctccac gatgattatt tctttgtctg cagtctctat
     1021 ctctatcttt tgatattctg tgttttcttc caatatctct gttaccacac ctctgttctc
     1081 aacagcatag gctggcgtag ttattagggt aaacaacaac accagccatc ctaagatctt
     1141 atacatatat ctagaatttt atcattctag gacacgaatt cttttagctc ttgagcaaac
     1201 atttcttttg gtctgtaacc gacaaagtct ttgattacct ttccttcttt gaatagtttc
     1261 aaattaggaa tgctttgaat catatactct atggctaaca cttgattgtc tggttggtca
     1321 gtgtccactt tcacaatttt taatttacct tcaaactcgg tggccaattc ttccagaact
     1381 ggggccatca tttgacaagg tccacaccat ggagcccaga aatcaactaa cactggtgtt
     1441 tcagacttta atacttctgc ctcaaaattt acttttccgt ttacattaat tggacttgac
     1501 atattttttt aatttaatta ataatttttt atcactatca aattggcctt cgactatttc
     1561 gtccatcacc ccactcacag ctgatctaat tgctttcagt tgggtcaaga ctttttcaca
     1621 atcgcattca ttatcaatca ttttctttac cccttcaatt tggccggaga tattgtttaa
     1681 tctttgtgtc attgacttca taatccaatt atacagtaca ccccctgggg gtgtcaacaa
     1741 ccttgtttta tgtgtacctt cataatacac atatccatga gtacttccat attttttgat
     1801 tcggctattt tctttgcttc tggtgacacc accccctctt gcatccaaat tatcacgtgt
     1861 ctttttgact cgataatatc tttaacaatt gtttctactt cctctgacct tctaaatata
     1921 tctactatat ctattactat ttcttgaggg atctctttta tcgacttata tgcttttatt
     1981 cccaacacac tatctatagt tgggtttatc ggcaatatat taaatccttt tttaagtaag
     2041 tattcagcga cacggtagct tggcctctct gggttgtcag ataagccaac tatggcaata
     2101 gtttttatat ctttccaatt cattaaaata ttatctcatg gtgttaatat atatgttata
     2161 tgaccaaaaa aatctctctc cttgctctaa ccgctatttt tttagctgcc tgcactagcc
     2221 cttctactgt ccaagataag gttgaagata aaatctctga aaaagtcgac aaagtagaga
     2281 ccaccaaaaa atcactcaaa gatttgctta gtcttggtgt tgctcagaag tgtgtttaca
     2341 actttactga tgaaggagtt gtctcaacag gcgagttact agttagcggt aacaaattta
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnatgta cacttggaat gataaagtac ccaattctgg
     2461 cattaagttc aaaattgaag aaactgaaac tgcaagcgat tcaaaggctg ttccaaacga
     2521 atcagtagat atggaaaaac agtatgatta taactgttct cctacaactg tctctgatgc
     2581 tgattggaag ttaccagccg gcatgcaatt tgctgacatg gcagaattat caaaatttgg
     2641 tggtatggat ctagaaaagc tccaacaaca atttggcact gacgatcaat aacttctaag
     2701 gactgactct attaatgctt tgttttcttt tgtccagcta attaatcctt ctagtccctg
     2761 gtctacacta atttctggtt tccaccccag ctctttgctg actttaccaa tgtcactaac
     2821 gtaaactttc tgatctcctg gtctccaggt ggaatagatt ggctttattt tcggattcaa
     2881 tttattcaat agttcattta ctgataaaga attgtcagtc cctcctccaa tattgtagac
     2941 ttgtccagaa gttgttttta tttttacaaa agctaggtca tatgctttca ccagatcttc
     3001 tacccacaac acatctcgga cttggtatcc atcaccatag acagtcgttg gcttgtttaa
     3061 tattgaagca attgtgaacc aactaatcca accttgatcc tcaatcccaa attgacgtgt
     3121 tccgtaaata caactttgac gaaacactac cgttttaagc ccatatatct ttgaataatc
     3181 atgaacatat tgttcagctg ctcctttgct gcagccgtat ggtgaatgaa agtccaagtt
     3241 ctgtaactca ctaattcctt tcggtcggtc agtatatcca tacccgtgtt cgccttttat
     3301 tactcccagc ttttccattt ccccatatac tttgtttgtt gaggcataga taactaccgt
     3361 ctcaggtgaa ttatttctca ccgactccaa cagattgagc gttcccattg cgtttatttc
     3421 aaagtcttct cgggggtttt tcaccgaagt agtcaccgcc acttgagctg ccaagtgaat
     3481 tacgaggttt tggtctttga cctcgtcatt caacttcccc tcggttctta tatctccttc
     3541 aactatttta atttctttgt ccccatattt ttctttaagc caagtgatgt tctctctagt
     3601 tccttttcgt gaaaagttat caaatattgt tacttcgtcc ccattattta aaaaataatc
     3661 tgctgtatgg gttccgatga atccagcacc tccggtaatt aaaactttat tcatgttatt
     3721 attatatata attcatccaa taagtccgtg atataatcgc ctactgcttt tatcttgata
     3781 agagatctag atttggtatt tattaatttc ttctattgtg caagttcatc cttccctaaa
     3841 tagtattagc gcccaggtaa tttttgactc agccgtcaaa aaaggcgtca ctgttgaagt
     3901 catctcttct cgttttaatt tatttaaact aatttataaa gaaaagtttc tttttgtcaa
     3961 atctacttcc cttccggtca acccccaacc ttcgtgtgct attgctaaca ataaatacct
     4021 taccaagaaa attctcgaag cccagggaat tcttatgcct caaggttggc ttgtctctac
     4081 tcagcaagag gctcgagacc tgattttaga aaaagacatt ttcccttgtg ttttaaaacc
     4141 agctcgaggt gctcatggtc gccatgtcta tgccaatatt gagtcaatgg cagagtttga
     4201 acatctttta ccactggtct tttccggcca tgccgttaag aatgttctta tagaagaaca
     4261 tatttctggc catgattatc gcctgctcgt cactggtaat aaagtctcgg ccgttatgga
     4321 acggattccg gctcgtgttg tgggtgacgg tgttaatact atcagtcagt taatttctat
     4381 cttcaacaaa agtcctcttg ttggtaaaaa atacgagagg ccactttgta aaattgtcat
     4441 gaatggtgaa gtttctcgca atttgcaaaa acttagcaaa aattttaccg atgtcttaga
     4501 agaaggggag acggtttttc ttcgccaaaa tgccaatatt agtaccggtg gtactggtca
     4561 tgatgttacc gatagtgtct gccagtcagt taaagatatc gccattgctg ccactaaggc
     4621 cattggcatg gtcatcagtg gggttgacat tatttacaac gaaacctcta agaaagctta
     4681 tgtactagaa attaacgaca cccctggtat tgatattcat cattatcctg tcgccggtgt
     4741 tcctcgtctg gtggccgacg acattgtcga caacctcatt gagcagctga aagtctaaaa
     4801 catctggtgt atattgcata tatatcattt gattctagac aaacccgttt tttttgtgta
     4861 ccatgcttct gcatgtccgc attgttcact gtagttaatt cacctttaag atatggaaat
     4921 tagtgttgcc gccgaacaag taggaactct ttttggtatt gttccgatta ccaactctct
     4981 tttagttacc tggatgatca tggccgtaat tttggtcatc agtatagtta tcaaaacagg
     5041 cattaacatt attccctcgc ctattcaaaa cttcgctgaa ttcttttacg aggaaagcta
     5101 taagtttgtc gaaggtgttc ttggagaaca cacttctatt gtctatcctt ttgctattac
     5161 ctttttcttt tttatcgcca tcggcaacct agctggtctt ttgcctggtg ttggttcagt
     5221 tactattgca gccgaacatc atggagagca tgtcttatta cctctctttc gagccttaac
     5281 tgccgattta aatctaacct ttgctttggc tatgattgct attgttgcca atcaatattt
     5341 tgctatcaag gtcgttggta taaaaaaata tcttcacaaa ttttttgact tttcttctcc
     5401 gattaatttc ttcgtcggca tgctggagat agtttccgaa ttttcaaaaa ttttatcttt
     5461 ctcttttcgc ctttttggta acatctttgc tggcgaagtc cttcttaccg tcatctacgt
     5521 tctggtacca ttttttattc caataccttt tcttttaatg gaaatctttg ttggactaat
     5581 gcaggccttt gttttctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatat
     5641 ggatccacaa ataatcgccg gcttaacaat tgctattgga ggtattgccc cagctatcgc
     5701 tattggcatg atcgccagta aaggtcttga agctattggt cgcaatcctg aggctgcatc
     5761 aaaaattcaa acaaacatgg tcttgggctt agctttcgcc gaagctatcg ctatctattc
     5821 tttagttatg gccctaatat taaaatttgt ttaaactgtg gaaaaactag gtatcaaccc
     5881 catccttctt ggttttcagc tattcaactt tgccgttgtt ttagttttgt taaattttct
     5941 tgttttaaag cctctttctg agctaatgga caaaagaaag aaaaagatcg aagctggtat
     6001 cgccaaggaa ggggaagttg acgttagact agctcaaatt gaatcagaga gaaataaaca
     6061 attggccaag accaggactg agattgacca aatgatggct gatgccgcca aaaaggctgt
     6121 cgtcattaaa gaagaagtct tggctaacgc cagaaaagaa gccgactcca tcgttaccaa
     6181 ggctcaccaa cgaatatcta ttgaaaaaga tgaaatgatg gctactgcca aaaacgaagt
     6241 cgctgaatta actattaaag ctgttgaaca actgtttagt gatgaacaat cttcaagtgt
     6301 cattaaaaaa caaatcaatc aaagcactat tgaaaaacta tggcagaatc accagaagta
     6361 agaacactcg ccagacgtct gtttgaaaaa cagatcgagc tactttccca agtccccgag
     6421 gctcagcgtg acagtttgcg tctacttata ttcctctcag ttcaagcatc gcttgaatta
     6481 atgaaattgg ttatgactac tggcaacact ggtttagcca accaagtcgt cgatgaactt
     6541 gaagaaataa tgagagaatc tttgcctaaa atgagcgtta gtgttcgctt agcttcatct
     6601 ttaaccaaag aagaagaatc aaagctgata aaaattctcg agaaagagac tggtaaagac
     6661 ataattctca atactagaat cgacaaaaaa cttcttggtg gcatggtctt ggaatatgac
     6721 ggtaaagtcg ttgatcttac cgttaacgat catctctcca atttaaaaca acatctttta
     6781 agttagccca gtatgacaat aaaaaaaatg gatatttttt ccgaactaaa aaaacaaatc
     6841 gctgactatc aagagcaatc ccaagtcgat caggtaggtc ggattatgga agttaaggat
     6901 ggtgttgcca aagtacttgg acttgccaaa tctaggatgg gggagtttgt tcttattggt
     6961 caacataata tttcaggcat gatactttct ttggaaagaa atatagccat ggttgttatt
     7021 atgggtgagt ggagcttggt cactgaaggt gatcaagttt ctggtactgg tcagttaatg
     7081 tcggttggtg ttggtccaaa attacttggt agagttatcg atgccctcgg tcatcctatc
     7141 gatggtaaag gtaaaatctc aattgaaaaa tatattccca ttgagcgcaa tgctcctgga
     7201 gttatcgacc gtcaggcagt caacacttct ctggccactg gcatattatc aattgatgct
     7261 ctggttccaa ttggtcgcgg ccaaagagaa ctaattattg gtgatcgttt cacaggtaag
     7321 actgctctgg ctattgaca
//
LOCUS       DDWP01000042            5047 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_10825, whole genome shotgun sequence.
ACCESSION   DDWP01000042 DDWP01000000
VERSION     DDWP01000042.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_10825"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1285 a         1101 c          769 g         1892 t
ORIGIN      
        1 cttgttggta ctttatttct cttctaaaaa agcgcgacga tattgccgat attgcttggg
       61 gcttaggttt tatcattgtt gcttttttta atttatctac tggttatatt aacccccagt
      121 cgctggtcat attctttcta gtttctattt ggggtagtcg gcttactttt catatttatc
      181 agaggaacaa aaataggccc gaagattatc gataccaaca gttcaagtca aacccttatt
      241 ttaaagtgtt tatggttcag ggatttttta tgttgcttat ctcagctccc ataattttca
      301 ttagccgctt tgatatcact ggttggggct acatcaatac tgttggccta gttctctggc
      361 ttgtcggatt tttctttgag acagtcggcg attcccagtt aaagacgttt gttaccaatc
      421 ccaataataa aggtaagatc atggatcagg gtttgtggcg ctacacccgg catcctaatt
      481 attttggtga ggttgtcatc tggtggggga tttggctcat cagtttttac aaccagttta
      541 gttttatctc aattattggt cccctcacca ttactactct tattcttttt gtctcaggtg
      601 tccctatgct tgagaagaga tatcaaggcc atcccgagtt tgaagaatat aagaaaagaa
      661 ctagtatttt tataccctta cctccacgaa aatgaccgaa ttcaagccgg aactgactcc
      721 caaagaaatg ttggagttgg gtgtttttgg tggtaaatac atgtccgata gacctagtga
      781 atttcctgct gattggttta ctaatgccaa actttcttcc gataagcatg atcccaagtt
      841 aaattatttt ggtgttaacg ccagccagcc actatctgtc tggcgcgata aaggttggat
      901 tcaccccgac gacccccgag gttggtttca atggtattgt cgttattttt taggtcgtcg
      961 ccatcccgat gacgagcgac aaattaaacg ctggaaagcc atgtcgcgcc atgtagccca
     1021 agtcaagaga tactgttctc cttgtgattt gtcttgccga cctcgtcagc gtcaggccct
     1081 tcttcattgg gcctacgaca gtcgtaagat ctgatgatat attaataata tgcaaaaact
     1141 aatcgacact atttctaatt tttttattca aaaatctcca cttattatca aatggctgcc
     1201 tttagtttat tttagttttg tcctgtttgt ttggtttaat ttaaacactc gtcttggtta
     1261 tgggttaagt aatttatttg gtgagatcgc ggttggactt tatgttttaa ctctgattcc
     1321 tggtattact ggcaggcttg gtttaaagaa ctctgtcttt aatgtcatta gagtctatcg
     1381 tcgtcatctt ggtatatcca tgtatctttt tgctttttct catttcttta ttaagagatt
     1441 tccttttact tttcctccat cattttttgc ctttgaagtt gttggcatgc ttgccatttt
     1501 cgtattcttt cttttgtttg ttacttctaa taactttagt caaaaattat taaccaaaaa
     1561 ttggtttcta ctccaaaagt taacttatct tggcatgttt tttatttttc tccatttggc
     1621 ttttcttaag gtagctacct ggggaactct catggccttg gtgattattc ttgaattaat
     1681 ttctttccgc tttcagaagc gattcgttta aaagtcttgt acaaaataag ccaggtgtcc
     1741 agcgacactt cggttggttt cttgccggca attttataca ttttttcaac tcttttttgt
     1801 tgtgggtaag taaacgattt tagaaaatag ttaaagttgt agattacaaa tttcttaaag
     1861 ttttgtttat cctcttcttt tataaaagct gactttcttt ttgtaaactt taccaataca
     1921 atttttactt gaggttttag agtaaagttg gttctatcta tatctcctaa tatttccacc
     1981 tcataccatg ggtagtagag aactgcactt tgccctttat tagcaaactt ttctgccgcc
     2041 tcattttgca ttattaaata catttcttct ggttgactag aagaactcca aaacttatcc
     2101 attatttctg ctgtaattga gaaggggata tttgaaaata ctttatattt ttctgtcggt
     2161 aattcaaagt ctagaaaatc tgccacaatt gctcccgttc tttgtgccaa tgctgaatcc
     2221 ttttcgacag caattacctt cttggctact tttaccaatt cttttgtaat aattccttta
     2281 cctgacccaa tttccaacac tgtgtcgtct cggtttatgt ttgatgccga caataactcg
     2341 cgcaccaaac tcgggtactt aataaaattc tgtcctaacg aactcttatc gtggtatggc
     2401 atgaacttat tatatcttaa cctaacccac ctcttcttta ttatttttgc ggcagcaaat
     2461 tttctccacc attattcctc acatatcctt caaccttcat tacaaattta tctttattat
     2521 taaaataata gtcttctagc attcttttct cgtttgcgtt taagctgaca ggatctctcg
     2581 catcacttac gcgaggtctc aatgcagctg gaagcatacc aaaatgaata ccccaagcat
     2641 aaatttcagt atgatgaaat ttttcatagt ttctttcaat atcatcgtga cttaaatttc
     2701 tccaataatt aagttccctt tccaaacctt caggcgtata tcttagaaaa tttttcataa
     2761 aattatccgc ccaactcact acttcttgtt ttgggacaaa cgtcggtact tgagccaaaa
     2821 tatctacacc catatttttt attcctcagg tggtaagaat tcctctccgt tatcagatac
     2881 tgtctgtcct ggctttagcc ataggacata ttcacatcca gttgctttct tagctgcact
     2941 gtcccatcca ccagtcgaac acttcttcat tttcttacca gcaacagttg taaaaagtac
     3001 taatggctct ccacattttg gacatgtttc gtctagtttt tctgttgttc catttatcca
     3061 ttccacgtaa tcacatccta cggcaatttt cttatcttta tcccaacttc cagcactaca
     3121 tttcttcatc tttttaccaa atctagtcac ctgcattatc aaattagcac cacattttgg
     3181 gcatttttct tccagttctt gtgggggcac ttccaaccat tttacatatt cacaaccttc
     3241 atttttatgt gtttgtggat tccaagatcc tttactacat ctttgtagct ttcttcctgt
     3301 agctgtttca gtaacctcac ctagatttgc tccacatttt ggacagacgt cattttgttc
     3361 attcatatgt tctctagtat acattatctc ctgacgagta acaccccaaa tggcagatct
     3421 ctccctggtc cagctaaata tttcccatta ctccacatgg ccgacgatcc accactgtct
     3481 aaattaattg cgtttttaat ccccattttg gcaatcactc tagccgcttc tgccactgtt
     3541 gccgagtaga ctattccaat atatacagta ttaccgctag ctccaataaa tgatcgattg
     3601 cctctggccg attctttagt atcgtcatta cctccaaact taacttcacc gttaaacact
     3661 agtaacggtc gccccattaa cactgcatca acgctggtat ctcgacccca ttcattacct
     3721 ttttccataa atctcgacgt tcctgagaat actgccaccg gattactact atagacatta
     3781 ttatctgaat taaagtagat tttgttttta ttcattagca gtgtatcaaa agagtttttc
     3841 ttttcagaac agctggggta cgttgctgga caaaaataag tactatttat agccgcaaat
     3901 gcccccgacc ttttggctaa ttctcctagt gacattaccg ggcaatcatt tttacagtca
     3961 cttgttgcgg ctgtatcgat tattacttta gtattactca aatcagccga gatcacgttt
     4021 actacaaagt caccattatc tgtagtcacc tttgtaccag agactggggc tttcttgacc
     4081 acgaccaatg atgcttctag tttggttatt tctttgttta attcatcagt caactttact
     4141 accgtttcat aatccctttt ggctagcgtt gaaataattt ttgcccaaga tatttccagt
     4201 ttctttgtgt caccattttt atctcttaaa tctagtaatt tctcatatat ctctacactt
     4261 tttaaatatg tcttttgaat actgtcaatt tctgtagtta gatttttatt aattaatatt
     4321 tggtcagaac tctgtaactt ttcataatca gttttcactt gttccaattc tttttcaacc
     4381 acttgtcttt tttcatttga cattttaact tgagcatagg caaaattagt acctagggca
     4441 attaaaccaa tcaatactat gacaattatt ttcttcattt gtaaaggctc aagatcatct
     4501 tgtttggctc tgttccctct ctttcaaatt taaaaccaag cttttcataa gtcttaactg
     4561 ctggtaggtt atctttcgat acttccaacc aaaaagattg actttctcct ttactgtatt
     4621 cttccgtttt cataaatcta tccatcgttt cttttagaaa ccatcccgct aagccttttc
     4681 ctctagcctc accatacagt ctaatagcta gagtaaaacc accttcactg tagccaaacc
     4741 aagttattcc aatgagattt tcactatcat ttactaagct atatattgtt ctcccttttg
     4801 atatccatct gtcaaaactt tcttggctcg caaaccttgt tctgtcggaa gtaaactttg
     4861 ccacatccag atcattagca ctataagcta tcagttcatc tatatttttc ttatctatac
     4921 catctcttat ttccatagct aaatataaca aaaattgtta aaattaggcc atgcagttag
     4981 ttaaccaggc agtcatactc gccgctggcg agtgctctcg tttctttccc tttactcgta
     5041 atcagca
//
LOCUS       DDWP01000043           19008 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_290, whole genome shotgun sequence.
ACCESSION   DDWP01000043 DDWP01000000
VERSION     DDWP01000043.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 19008)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19008)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19008
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_290"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    5419..5433
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8383..8578
                     /estimated_length=196
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10245..10274
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5537 a         2778 c         4832 g         5620 t
ORIGIN      
        1 ccaggtagtc taatcttgcc ggaatcatag agtttggtga tagttttctc agaaattcct
       61 aagatttcta gagtctgaca aaataaattc aagcaaccga taatttgagg aagacagtta
      121 atgttcctac agcggagaag tttgtcttcg acagttaaaa gagtattgca tgatggacag
      181 tgggagggaa tgtcgatatg tcctggtttt attttggtga tgacttcctc aacatgagga
      241 ataacgtcac ctcgacggct aatcttaata atatcaccga tgtttaagtc cattttttta
      301 actagatcgt agttggcgag agaggcaaag gtaatgatgg caccagagag ttcaattggc
      361 tcaacttcag cgacgggagt aatggaacct aaaggtccaa tttgccaagt aatggatttg
      421 atgacagtgg tgttggtaga agcgggaaac ttataggcaa cttggaattg aggtcgattg
      481 tttttgacgt cgagactttc gactatattt agatcgttga ttttgacgac tagtccgtcg
      541 atctcaaaag gataagtgtt tctcttggtg gtgaagaatt cttgatatat tttttcgata
      601 tcgtcaaagt cttgacaaac aactgtttcg acggtggtga agccaaactt ttttaataaa
      661 ttaattttgt ccgattcatt taatgatacg gatatgtcga cagcgtagag agaacaatat
      721 tcagagaatt ttgaatcgag gcgttgggag atgccggagg cagcgttacg gggattggaa
      781 taaaaccctt cattttcaat ctccccctcc gacgttcgtc ggagaccccc ctttgacaag
      841 gagggtaagg catctttttt aataatttta tttagttctt caaaatcttt cttagtgacc
      901 ataatttcac accggatgga gcaagacaaa tccttcggca agctcaggac aagcccccgc
      961 attttaacga cgttttgggt aattacgtcc cctatttgtc cgtcacctcg ggtaatggca
     1021 tcaactagga cgccattttt atattctatt tcaacagaaa taccgtcaca tttaggttgg
     1081 accacgtaag atatgctgcg catatcaacg tggctgccta cggcgctttt ttttaattca
     1141 aaatagtgga tgaggtcgga ataattggta actttgttct gggaacccat gggcatggag
     1201 tgcttttttt tgtcaaagcc ggtattgaag tttggattgc caactttaga aaataatcta
     1261 tggtgtgggg aaattttacg taatatatct tcgagtgtat cgtaggtgtg atcgtccatg
     1321 atgggcttac cactggtgta ataggctttt ttggcttcga tcaaaagctc gccgagttct
     1381 gcaggggtag ttttttgaag gtcgatggaa tcgagagtga gcatggtgta agtttatcaa
     1441 gtttgtaaag tttataaagt tcaataagtt gattttgctt attatagggt gtaaaatgtt
     1501 cgtatgagaa tcagtaaaat agcttatgct ttagcgattg cagctgaaag agatccggct
     1561 agtatacaac cggttggagt ggagggtgag ttgcatttca gggttggaaa tcgagtttta
     1621 ccagaggatg aggcaattaa agcagcagaa cctgtggtga gaggtatgaa tattgctgtt
     1681 cctgtaaatt taccgttttt tgatgattca aaattaggtg ttgagcctga cattgaggca
     1741 gctaaaagaa gactttctgg cgcaataaag agaaggttga gtaatgctag gtaagtagaa
     1801 cagaacttgt tttgtgttta aacattagtc aaacagtagt aggcgccttt ggtggagcca
     1861 cgtttctcgg caactttgtc atcaattagt ttcttaagat cacgccagat agtatcatcg
     1921 gaaaccatgg gtagcagggc cttagcatcg ggcattctta gtccaccaaa ttgttgcatg
     1981 tattcaacta ttttgatctg cctttcggta agatgaattt gtttgccacc gagtttttgt
     2041 ttgaatttaa tatctcttga aagcgattgg actttttctt tgactcgatt gagctcgata
     2101 cttaaagctt cggtaaagta ggcaatccaa gaagttaagt cgcgcttgaa aatatcgcta
     2161 ctgagctcgt gagttgattg aatagtgttg taatataatt cggcatttcg atcaaagtat
     2221 tcttccatgg aaaaaagacg acgaatatcg taaccttcga catatagagg tagcatagca
     2281 aaagcgcgag aaacacggcc attaccttca ataaaaggat ggatggccac caaaatatat
     2341 tgaagaatgg cagagcggat ggcgggatga atttcttttg agtcgggggt gttggcccag
     2401 tcaattaaat cggccatgag atagggaact tcgatggctg gtggtggtcg atgagcgatc
     2461 tcgccagtgc gagtgttttt gagaacaact tgttgacgac ggtatttgcc gacatctgat
     2521 tcgggaataa ttttgaaagt ggtaagttga tgaacttttt tgactagttc ttcggaatag
     2581 gaaaaaggaa tagtggtctg cttgacttct tctaggtaag aaacgacatt tcgataattg
     2641 atgacttctt gaatatcacg gtcggtggcg gtaatatttt gaccttcaat tagtttggca
     2701 acttcttgaa aagtaaggtc gtttccttca attcgggtac caaaatggac tgtacggaca
     2761 atggcgtcat gttgaaattg cttctcgtaa gctggaatta aaggtgcttc gtcgataatg
     2821 gctttggcac cttcaatagc accgatgtgc ttgagaatgg tgttattgat ggaaaagact
     2881 ggtgaaaaca tctatcctta ttataacact tttttgaaat atcaatgagg gaaaaatgcg
     2941 gaaatcaatc tccccgctcg cttcgctcac acccctcttt gacaagaggg gtaaatttag
     3001 ttaagaaaca gaaaatccaa aactgttttg gggtcgaggt tggctttgag cattttggtg
     3061 gctttgatga gcttattgat ttgcaaaaag ttcttgggac tggtgacttt gatttggttt
     3121 tctaaaaggg gcaaaactag ctctttgttg actgataatt gttctgataa aagtaaagct
     3181 tcggcggtgt ttgtgggaaa aatgaatgga ggtgtggtgg tacttgtgtc gttaattttg
     3241 attaagtggc aacgggaaat gatggtgtta ataagagatt gaggtcgtag agttaataga
     3301 aagaaataat tattgtcacc tggttcttcg aggtttttga gaagagtgtt ctgtgattct
     3361 ggacttagat tgtcagcttg gggaataata ataacttttg aatcgtgtga ataaggagac
     3421 atggataaaa agttactaat agaacggact gactctatgc tgtagtcgct gagtatgaaa
     3481 atatcaggat tgttttctaa agtatcgtgg ccaatttctt ttaaaaatgg gtcaagagaa
     3541 gagagagccg cagaagtgat tagtgtcgaa ggaaataaca taatgtatat ttattatagt
     3601 ggataaaaag atatataaaa atttaagaga ggttttactt gatcagggta agattgatgc
     3661 ggctaaagca gaggaaataa acctaaggca agtaaagact ggtgaaagtg aagaagagat
     3721 agttagggca atgcggataa tcgatgagga aagcttggtt aaagctaagg cggaattgtt
     3781 gcgggtagag tatataaact tggattcgat tggtttctcg cctgaggcac tggctttggt
     3841 gccggagtcg gtggcccaaa agtataaggt agtcccctac tctatggatc cgcgtaataa
     3901 gatattgtcg gtaacgatgg cgaatcctct agatatagaa actatagagt ttttggaaaa
     3961 gaaggctgga atgaaggtgc gggcggcgat atcgacagaa aaacagatta atgaatttat
     4021 ctcggaaaaa tatgttcggg agaaagggat aacttccgaa gtgtctaagg ctctagatga
     4081 acggaaaaaa gatgaaggta aaacattggc tgatacggcc aaaaaagtgt cggctgaagc
     4141 tccagtggcc aagatcgtga cgactatttt ggaatttgcg gtaaagtcga gagcatcgga
     4201 cattcacatg gaacctcagg aagataacgt gagaataaga tatcgaattg atggaatttt
     4261 gcaggaaaag tatatgttgc ctagaaatgt gcatgaggcg gtagtgagta gaattaaaat
     4321 cttgtctagt ttaaaaatag atgaaaaaag agtccctcaa gatggacgct tctttttctc
     4381 ttctgatggt aatgacgtag atttgcgcgt gtcgactcta ccaacgactt atggagaaaa
     4441 agtggttatg cgtttactta agaaatcaca aaaggtacct tctttgccag acttgggttt
     4501 aagaggattg gctttaaaaa atttgatgga ggcaattgaa aggccgcacg gaattattat
     4561 tgtttgtgga ccgacaggat caggtaagac aacaactttg tattctgtcc tggataaggt
     4621 ggcaacgtct aaagttaatg tggtgacgat tgaagacccg gtggagtatc aaatgaaagg
     4681 agttaatcaa gtccaggtta atactcaggc tgggctgact tttgcttcgg ctttgagatc
     4741 ttttttgcgt caggacccta acgtaatcat ggtgggagag gtccgcgata cggaaacggc
     4801 tgaattggct attaatgcct cactaacggg acatttagtg ttttcaactc tgcacacaaa
     4861 tgatgcggcg ggagtcccac caagaatgtt ggatatgggg gtggaaccgt ttttgctggt
     4921 atcttccttg acctgcgtgg ttggtcagcg cgttttgaga aaggtgtgca aagagtgcat
     4981 tggtgagact gagatgacac cagacattga gtcagaaatg aaggcaacct tgggtccaat
     5041 ttataatatg attacggaaa agtgggctaa ggacggtaag acagttaaaa tgccaaaagt
     5101 ggtcggttgc gaaaagtgca ataacactgg ttattttgga agaatcgcta tttatgaggt
     5161 aatgccgatg acggaaaaaa tagctaagtt ggtggtggaa aaagcagctg cttctgagat
     5221 acaaaagcag gcaatgagtg atggaatgtt aactatgaaa caggatgggt atgtaaaggt
     5281 actagaaggg gtaacaacac tggaagaagt gttgagagtg gcacaatatt aaacatcaaa
     5341 acttaaaact taaaacataa aaatgaatac aaaaattaca caaatattgg aattagccgt
     5401 agcaaataat gcgtcggann nnnnnnnnnn nnnagcgtgg atatgggtag tcctcaggtg
     5461 gcaacagata cgatactttc gactttaagc caacaacagt tggagagatt taaagccgag
     5521 aaagaattgg attattcatt gtctgtaggt gggcatcgtt tgagggtgaa tatgtttttg
     5581 aaaaatggag aagtgtcatg cgctatgaga atagtcccct tggcaatacc tgactttggt
     5641 accatgaatc tgcctagagt gttgtctgat ttggctgaat acaaacaggg attaattttg
     5701 gtggctgggc cgactggcca tggtaaatcg actacagtgg cggcaacttt gaataaaata
     5761 aatcaagaga gaagctgtca tatattaaca atcgaagatc cgattgaata tgtaataacg
     5821 ccggtaaagt cgataatatc gcaacgtgag ctgggtagtg atactgatag ttttactatg
     5881 gccttgaaat cggctttacg acaagaccct aatgtagtat ttgtcggaga gatgagagat
     5941 ctagaaactg tcgccttggc cttgacggtg gcggagactg gacatttagt gttttctact
     6001 ttgcatacaa attctgcttc acagacgatt gatagaatta ttgatgtttt tcccgaggga
     6061 tcaaaggatc aaattagagt gcagttggca agtgtcttga cggcggttat ctctcaacgt
     6121 ttagtaccgg cagttaatgg tggaagagtg ccggcttttg aaatattggt ggcgacttcg
     6181 gctgtaaaaa atgctattcg tgaaggtaag tcttttatga tagataatgt gattcaaact
     6241 agttcggact tagggatggt tagtttggag cagtcactgg caaacttggt caaacaaggt
     6301 gtggtggctg aggatgtggc aatgagctat gccctaaggc cagtggagtt acaaaatagc
     6361 ttgagaagca aacggatgat atgagcaaat tccagtttaa agctaaagat tggaacggga
     6421 aagtagtgag gggtatattg gaaatggcgg ataaatcgca ggtaattgag tcggttaaaa
     6481 gtaatggatt aatattgctg tcagtagagg aagaaagaga taatgtattt agagaaatac
     6541 atagaaaact ttttgtaaga gtcgggctga aagatgtgtc ttctttaact agacaattgt
     6601 cgacaatgat gacggcgggg ttacctttga ctgatgcttt gtcgttattg aaaggtcagg
     6661 ctgagggtaa tatggcggta tatgaaatag tggattattg ccttaatcag gtacgaggcg
     6721 gccaacctct aggaaagtct ttggaaaaat atactaatgt ttttggagag gcatatattg
     6781 cttcgattag cgctggagag gaggctgggg tgatggaaga agtgttgtca aaactagcaa
     6841 aaaatttgga aaatcaggat gaatttaatg gtaaagtcaa aggggcaatg gtgtacccag
     6901 ttattgtggt agtgggaatg ttgatagtgg cttttattat gatgatattt gtggtgccaa
     6961 aattgcttgg gttgtatgct gattttggaa cagcaaaatt accggcgacg actaaggcct
     7021 tgatggcgat gtcggatttt atggttgatt ggtggtttat ctttcctatt ggagcaatgg
     7081 gtttgtatgg cttgttgaag gtgggtgata aaaaccctaa ctttagacta aaaagagata
     7141 gcttaaaact caagttacca gtactgggtg aattgactaa gaaaacaacg ctttctaata
     7201 ctattaggac tttatcaatg ttgttggggg cgggtattag cttggtggaa gctttaagaa
     7261 tagtctcaaa ggtagcgggt aatgaagtct atgatcaggc atttattcgt atagctgaga
     7321 gagtccaaaa aggtttttca atatcgaatt cttttgaaga gacggctgtg tttccagtta
     7381 ttgtagaaca aatggtggca actggagaag cgacaggaaa attggatgaa gtattgttaa
     7441 gggtgtctga ctattttgct actgaggcag aacagtcggt caaatcgtta acttcagcaa
     7501 ttgaaccaat aattatgatt gtcttgggaa ttggagtagc ttttttggtg gttgccgtga
     7561 taatgccaat ttataacttg acatcgtcat tttaaaagta ttaaatgata tcagtaatta
     7621 aaattatcaa taataaaaac aacatgaaaa agatgtgggg aatagatgat ggtggtttca
     7681 ccttggtgga acttttgatt gttattgctt tgattgctgt gttatcagta gcagtgttag
     7741 caactatcaa tcctatcgaa caatctaaca aagcacggga tgctaaagtg caaaacgatg
     7801 cagctgaagt actaaatgct tatgaacgtt attacaccag tacaactcat tatccatgga
     7861 tggatgtagt tggaggaagt gctttgacgg tggatagtgc ttatcagggt agaagtattc
     7921 aagttggttt tggtctttgt tctcatggac ttccgttggc accaacaact ggttgtgaaa
     7981 gccaaacact ggcaggtcga ttgattgaga cacaagaatt aaaagattct ttcttaacta
     8041 aaacctatac gagattgtct gatggtagtg atcctgctta taattatcat gatgagctgt
     8101 atgtgtataa acaaccagca acacaaggaa actccatata tgtctgttat gtgccaaagg
     8161 ctagggcaaa tcgtatacct ccagccactt cttcatggaa gcttaaggct ttagcttttg
     8221 ctggtggttc agaaaatgca gctttaccga ctgcttttgt tgatgcaacc cctgaacaga
     8281 ttaccgcagc tacctatgac actttgcttg gacttggtgg aaatgcaaca ttgttccgat
     8341 gtgtaccgga atgaggatag tttaaattaa atttaaaacc cgnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     8581 gtatgaaaat attcctgtat tctcgtgggt gtttttaaga ggtaagtcga agtgttgtgg
     8641 taaaaaatta cctgttgaat acccgatagt tgaaatagtg attggaataa tatttttact
     8701 taattatcag tcccctatta atgtgattat cctgagttta ctggtgttta gtttggtatt
     8761 tgatcttaaa tatatgattt tgccagattt ttctacttat atcttgattg gactgtcagg
     8821 gttattaatg gcaatgaacg gacacacagg tccgttccta ctgtctggat tgggggcgtt
     8881 tttgttttta ctaattttgc atgtggggac gaaaggaaga ggaatggggt ttggagatgt
     8941 aaagttggct gtatttatgg gattgtactt gggatttccc ggtattatcg tatcttttta
     9001 tgtggcattt attgttggag cgatagtcgg agtgagtcta atgattatga aaaaagctgg
     9061 cagaaaaaca gtgatcccct ttggcccttt tctaatactt ggtacagtgt tagcaaggtt
     9121 tataattaaa atatgatgaa aaaaggcatg agtttgattg aggtggtgtt ggctttggca
     9181 atagtggcca tattgatggt gtttatgacg ataagtttga atccggcggc gatggtaggt
     9241 agggctaatg atgctcagag gaaaaaagat attgggaaaa ttaaattggc tatggaagag
     9301 cattacaatg acaagggttg ctatccgaca caggcagtgg ttgatgctat tggctgtagt
     9361 agtcgtgatt ttaatccgtg gctggaaaag tggccatgtg accctaatgg aagtcaatat
     9421 gtgattggaa tggaaaatgc gagttgtcct agttattatc gaatactaac aaatctggag
     9481 aataaacatg actctgatat tccaagcggc tggtatgaat caacactgat agcttattat
     9541 ggtgatggac tggtagacag agatatggtg aattatggcg tgtcgagtac taatgtgaag
     9601 tggtatgacc agatgttgag tcctgattgt gatactcctt ggcctcagtg ttatcgacta
     9661 actactgacg ggtgtacttc atggagcggt ggtcctcata ctgacgcata cgttcatcat
     9721 gattgtttac ctcagtgtcg agttagttgc tgcttggatg gacaagtttg taattaatag
     9781 agattaaagc tatgattaaa ctatgaaaaa gagcggtttt acattaattg aacttttggt
     9841 agttattgcg atcatttcaa tattgacgat agtggctttg gggcagtttc ggacggcgag
     9901 aatgagggct aacgatgtgg ctcgaaaggg agatttgaac tctgtctcta agtctttgca
     9961 gatgtatttt gcggattttg gagtgtttcc tgaggtagat gcaatcagct ggggaggaac
    10021 tttccaagac tcgactgtgt cgccacctta tatatatatg cagactttac ctcaagaaaa
    10081 tatactgtca gtcccctact gctataaaat aagtgccgat ggaaaaaaat atggattatt
    10141 tgctcaatta gagagccttc aagatatcca atgttcaaat agttatgtgt gtaatgggat
    10201 gacttattgt tatggaatta caagtccaaa tactagttta actcnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnngggttt actttggtag agcttttggt agcgatatct attatggccg
    10321 tattgatggc agtattgttg ccaaacttga tgggagcgag agagagagcg agagattcac
    10381 agagaataca aaacttaaat tcaatgaaag atggtttgag aatttattat aacgataacc
    10441 aagcttatcc gacaggaacg gcggtgatgc tggggagtgg gttttctggt tattatcaag
    10501 atgttgtctc tatcggttat acctatgact attatcagac aaaaaatggt gatgggtttg
    10561 tattgaccgt tggactagaa tccagggtac ctgacgcagg agaatctcaa cttaaatgtg
    10621 gagcgcagct gttaaatgtt tgtggtactg gcactgggac gacatgtccg gacgtgtttg
    10681 cagtatgtgc gaattaaaaa atcctaaatt ctaaactcta aattctaaaa attaaaacaa
    10741 aaaatgaaaa caaaaaatgc tgggtttacg ttgtttgaat tgttggtaag catatcgatt
    10801 attgcgatat tggtggcagt ggcgacggct tctttttctt cggctcaaag aaaagctaga
    10861 gatgcaaaaa gaatccaaga tattgaggcg gtgagaaaag cagcagaaca atactatatg
    10921 ttgagtaacg ctaattatcc gacagtatcg ggtgtgggag tgtcgtgggc agtaggtagt
    10981 cagactgtgt tggaatcgtg gccaagtgat cctaaagggg tgggttatac ggcttatgtg
    11041 ccgactttga cggcgtctac ttattgtgtc tgtgccgcct tggaaagcgg aaatggtaac
    11101 tcgacaactt ctggctgtaa ttttgttgca gcagggacaa aaaactggtt ttgtgttaga
    11161 aatcaacaat gataaagaaa aacttaaaac tcaaaatgca aaactcaaaa aagaatggcg
    11221 ggtttacgat gattgaacta atagtggtga tcactataat tgcggtgatg acaatagtgg
    11281 caatggtcaa ctatggtggt gtcaatagaa agtctcgaga tggtaggaga atggctgatc
    11341 tggaaaaata tagagtggcc ttggagattt ctagacaaat tgggtcgact tatccagcat
    11401 ctttgaatag tttggtaggt atgaatttga taaatgagat atcgactgat cctaagactg
    11461 ggtcggatta tacttatgtt agtactggtt atacttatac tttatcggca gttatggaag
    11521 atactggttc gactaacgta cctggagctt atcaggtaac gaatccgtaa aggactattt
    11581 acgaagtacg atttatgatt tatgaataaa aaaaacggtt ttacgctgat tgaactgatg
    11641 gtggcgatta ctgtgtcgat gataatgatg acaggtttag gactgtcgat taataattat
    11701 tataatgtag agaaaatcaa ccaaactaat ggagatttgg tgtcgttaat atccttggca
    11761 agaaatttgg cagtgacaaa tcagcgtccg gctggtttta ctgaagatct tgggagagta
    11821 gtagtaaatg taaatagtaa tgggttagta gaggcttttg cttatggtaa cgtgtcggga
    11881 atcggaggga gttatttttc taaagatatc gccgataatc gagtgtcgac aactatctcg
    11941 ggagatttgc ggtttgtggc tgggacaggt aaattggtag ataactttgg taacttattg
    12001 ccgtctagca cggagattgg gatagtcatt gcttctgaag atgtggggga gacaagacag
    12061 gtaataatta catctttggg gacaataaaa tgaaaggaca atctttaatt gaagttgtat
    12121 tctctatagg attggtaata ttggtaataa cagggattgc gatgttgttg gtaaatatag
    12181 tcggggcaag gacaaaaagt catgaaagat taagaacggt ggagttgtcg caggtggtga
    12241 tggaaaattt gttatctact aagaattcta atccgacaga gttttgggac cttggttcat
    12301 cgttctggat acaaaataaa gatgtgtctt tgactaatcc tagttttcct aattatgatt
    12361 atatggtgag ggtgactcca aactcgacta gtggttgtaa tccgacgagt tgttttgatg
    12421 tggtggtgag cgtggggtgg agtggtagtc ctgatgggag tagaaatgag tttaatagat
    12481 tctttggaag aatatagagg ggtaaagata aaactcaaaa ctcaaaactt aaaaatcaaa
    12541 atataagatg aataataaaa agacagggtt tactttgatt gaaatagtag tggtggtggg
    12601 agtgttgggg tcgattatcg tgactgtggc ttcggtacta ttaaactcgt ttaaagcaaa
    12661 agccagaata gaagtgactg acaaaattga aagaaacgga ggaatagtgt tatctgaatt
    12721 aaaaagtgga gtggtgaacg catcgggaat agatatgctt tgtgtgggga gcactttgac
    12781 ttatactagc tcgattgatg gtggagtgac aaatttggtt tgctatgaag gaacaaaaat
    12841 tgcctctgag tcggcaaatg gagtgtttga tttgaccagc agtgatgtgg ctgtgtcggg
    12901 gtgtaataat tttgtccagt gtgatctgta ttctggatcg acagatagga caactcaagt
    12961 ggattttgtg tttggactta ttgcgggaga tgctcaggct ggggccggcc agcaggtaaa
    13021 tcgtcagttt caatcgacga ttgtggtgag gaattagagg agtggcgatg gcagtgccag
    13081 ccccagtccc ctattggttt gaataaataa tctttggttt cgagactaca gtagaggcct
    13141 aattatgaag cataaaaacc acgaagatga acttgctgat ggtggcttat aacaatggtg
    13201 tcactgttga taacagtcaa taactgtctg ttctaggttg aggatagtga caattgcgct
    13261 ggtgaggaaa aacatgaggg ctgatggggc cgccagaatc tgattcgtta tttaaataat
    13321 aacttatgtt ggggagaaaa taaaactatg tgtattggtt atgtatataa tattataggt
    13381 tgtaaaaaag tagtactaat gatataattt atcaatgaga aaagaaatag aaattactgc
    13441 tagattgtca gtgggggcta tgcttatgtt tagtggttgt gcgccgactc gtgaggcatt
    13501 gatgatagat aggcaggttg ctgctggatt gggtgattac gctgagggag cgaatagaaa
    13561 tcttgactta gttgataagg cttgtaaaga ggtaccagga tatatttctg ggactgaaaa
    13621 gatagctcaa ttcgtgggtg ctgatggtag acttcatggt tgggcttttt gtgacggtga
    13681 cggtgggact tgggtggctg gacatatgag tgaaggtaaa acttttgtac ccgtagtaga
    13741 aagatttgtg tacactacag gtactgacgc ggcagggaac tccgggacat tgtttggtta
    13801 tgtacaagac ggtgaaactt ttggtgttta tgaggtagtt aatggtgtta gacaagttgt
    13861 ccatcctgat gatcctaata ttgtaaaggt tattccgaat ggtggtgagg cgaaattttg
    13921 ggaggaagtg gttaaatctg ccactggtgc cactgatgtt gctgctgccc cagaagaatc
    13981 tactcctaca gataagccaa ataccccgac ttctaccgaa gaagcatctg cgacggcgtc
    14041 ggctactaaa actgataccc cgactgctaa accaagtgaa actgcgactg gagaggtaga
    14101 tcccagtgag actcccttac cctctgatgg ggatataaca cttgaaaaaa tgataaagat
    14161 tcctaaaaat aagtcggaga ttaagacaag ttgtgcaaag tcccctgaca tgtttacaga
    14221 cagtgaaggg tttgagaaag ctctgacgga gtacacgaca ctcattgaaa caaatattat
    14281 tcctacacat acaggaccag ttctaacacc gggagttggt gttggtttgg ggtctgacgg
    14341 tgtatttttg actcaaggag cagaagttag ccctgtcgcg tgtttggatg ctgacggtaa
    14401 tttagtcttt agttttattg ctaaaaatgg tgataatact ttgtcagcaa tgcatgtggc
    14461 tttagataaa gatcaaaaaa actataacgt cagtagcaga attgctttgg ctgatgtttt
    14521 gaataacttg ccaatttcta tccaagacaa tagtactaca attgaacttg ctactaaacc
    14581 tgaaaaaaag actaactctt tggataacac caacttctca caatcttttt ggaagagtag
    14641 gggggaagtt catacagagg tttttgattg gcttagtggt aaacaaaaat ggaatagtaa
    14701 aaaagtcgtc ttgttgagaa ctttttaccg cgcttctggt ggtccttcac agatctcacc
    14761 tgtcgtgaag ccgtgatgta tagatggttg ctttcagtgg atctatatgg ttgaattaac
    14821 tagtgaaaat agacagagtg tatatcgttt atggacttat cgtattattt cttttggtag
    14881 gaatatttgc tttgtggcca agaaataaaa taagttttaa taaaattcaa gatatatctc
    14941 aaattgatgg tcctgttgta gaaattgcag agggtgataa gagagaaaca gctaagaata
    15001 taaacggaac tgtgcttaat tggaacggag agagtgatgt cttgagtgct aatgttgatg
    15061 gaaaaaagtt tgattttcaa attgatccgg aatttacctt ggtaactgtg accagagcag
    15121 ataataaggg gaaagaattc aaaatgaatg aagtaagaaa atctataaat ccaaaagaat
    15181 ggcaaacagc tttttgtatg ggggataaag ttgtcttgct actagactta gagacaacga
    15241 aagttaaatg gataatgaat actggggctc gagtgtgtgg ttataaatgg agatagaaac
    15301 tgttaataag ttgatgtatt agattgtttt ttgatgtttg gtgtggttga attatagtaa
    15361 tgaagttgga tagatgtcct gtaatttgtg aatttgtgga aataacaaaa tgaaagatgt
    15421 gttgataaaa tatttgttat tgtcggtggc gttgggaata atggtggtgt atgtatatac
    15481 gggtaaggtg gaaaaggttg attatagagt aatggctcag tatacgtgtt ctgccacttg
    15541 ttatgttact tacgcgacat gtgctgcttt gggtagggtt gatgcaagtg gaagctgtgc
    15601 tgcgggacaa gtctgctgtg gaggacccat accaactcca gaaccagtat gtaacgcggg
    15661 aagtacctgt atgacggctg gtggtggtga gacttgtgct actttgggac attatcctgg
    15721 tagtggaaca tgtatgtatg gaatttgttg tggtgctgct ttgccaacac cgacaccggc
    15781 ttgtacaggg aattgttatt tttcttatgg tggttgtggg gctttaggat tgcctagtgc
    15841 tccaggaggt tgttcatcgg gtctatactg ttgtgcagta cccaccccaa ccccagaacc
    15901 tcaatgtaac cctggaagta cgtgtatgac tgctggtggt ggtgaaactt gtgctacttt
    15961 gggacattat aatggtagtg gaacatgtat gtacggaatt tgttgcggag ctgctatccc
    16021 aacaccaact cctcctccgc tttgcccgat tgagtgtgaa tacaactgta ctaatgccgg
    16081 cttgagttgt gatgcaatgg ttaggtgttc acgagaaaat atttctgggt gtactgatcc
    16141 tggtgttgaa acgcagcctt ggacgagtgg gtattgtgtt gacgaatgtt ggacaggcac
    16201 ttcgggatgt gtggctttga catgtactaa tacttgtggc ttgggtggat gtccgactgg
    16261 ctgtggaagt attgataact gtggtggagt gagctgtaat gcttgtccag gaactgtcgg
    16321 aacgactcct ttaccaactc cacctggttg tacaccgaat tgtgcttgtg cagctaattt
    16381 gtgccataac caaacatgtg ttaataactg tggaacttct tgctatggaa ctttgtaccc
    16441 tggtaacccc tcggctccaa cattgagctc tccatctaat ggtgcggtgg tggcaggggg
    16501 aaatattaac ttgacttggg tggcacctat aagctggggg cagtgtccag cggctaatac
    16561 ttataccgtg tgtgtaggta ctaatttttt aagtccatgt agtggttcga tgttccctga
    16621 tttaccggca acttctctaa ccatggtcta taaccaatcg accgctggaa catattattg
    16681 gacagttggg gcaaataatg gaactggatt accgacagtg tattccagca ctagatcttt
    16741 tgtagttaat tcgaatccgg tagttaactc tttaactata gtaaattctg tgggaacaac
    16801 cttggtagca gaagcaggaa gtcggaatca aatatgtgat gcgactataa aaaatagttc
    16861 tatgccacga agagctacct ttatcattaa tgcttctgat ggtaatggtg gagcaaatat
    16921 ttctagtatg aatttgacat ggaacggcag aagttggccg gctgtacttc aggcacctgt
    16981 ggggaataac agaacaggaa tggtgacggt agattttgct agtggtgaca atgccacggg
    17041 aacatacccg ttagtagcga cagcaataga tgcttatagc gcaactggga caaataatag
    17101 tagatcgtgg aaggtgtggg attgtaacgt accagtgtca ggaagtgtct acgatagtag
    17161 ctcagaggcc ctaggggcag tgtgttcgac tggttctggg tttaatactt tagcctcggc
    17221 agcagttaat tttagatggg taagtgtggg agcgacaata gtaaatgcgg cgacggccaa
    17281 tacctattct ggactgaatc tgtcttgggg aggaaattat gcagtagtac cgaattctga
    17341 cttattagtg agtgggctgg taacgaggtg gattgacatg ggggtaggga caaccagctg
    17401 cggtgtggcg caaaacttaa ataatatgac tattgatcct tataatacta atccgcgatt
    17461 tcaggtagat ttctcaggaa taacaaatca agaaccatgg tttcagacaa gtggtggagg
    17521 tatacaagca gcgggtagtg tagaaaatat ggtaccagtc acttgtgcta atgaaccgtc
    17581 actttgtacg gcaagcatgt ctagggaatc ggcagttggt gcagcagata atggattggt
    17641 gtcgggaagt gctttaacaa ataattctgg ttgtagtatt actggatcgg gagcgatgtg
    17701 tgcttttggt gctcctaaca actggtacag aaacggatct tccttatctt catcggatag
    17761 atataattat cagtttttct atgatagata ttattctgcc ttaggactgg gaataacact
    17821 tggtccggcg gcaacgatgt cagatgtggt tggtcgaggg ggtactggag tattcctggt
    17881 taacggtgac tttaacgtag atgtggataa tgtcttgcca gccaatagtt ttttgatgat
    17941 agtgtcgaga gggacaatta cttttctaaa tacagtaaac aattctgcag gaatgtttgt
    18001 ggctgatagt ggaatcggaa cttctccggg ggtaaatact ggattagtaa tcgatggatt
    18061 gttatattct tcttctggta atgtccgttt gaatcgaggt tttgctgata agctggataa
    18121 taatactaac ccagcagtat tggtaagata ccgtcctgac ctattattta gtatgcctgg
    18181 taacttgttt agattgttat ctaattggag acagttctaa ataaaatcaa aacttaaaac
    18241 ttaaaacaca aaacttaaat tctaaaatct aaaatatgaa aaataacaaa ggacaagtgg
    18301 cactaatagt cctactattg tcggcggtgg caatgacgat cggactgggg atgtcgagaa
    18361 aaacggtggt agacacaaaa gttgaaactg acgaagaatc tttaaaacaa gcgtttaata
    18421 cggctgaatc aggggtggag tattatctgg gaactggggg aacacatttt accgctattg
    18481 ataatttgag cattgcagac atcgtagtgg gaaatattgg cgaaggctca acaataaact
    18541 tgaatcaata tactttgaat aattctaatt tggcttattg gttagtaggt catgatgcta
    18601 atggtgatat cgacgaggca gtagtttacg ggggtgatag cttgagtgtt tgtatggaaa
    18661 actcgtttga tagggcgatt aaagtggatt atttctatag taatgcttta ggtaattatc
    18721 aggttcaaag gtttggttat aacttaacac ttgatctggt aaatgggttt accaatgtta
    18781 caccggtgca aggtaattgt attagtggtt atagagaaca aagcttggga gtgacacttg
    18841 gtgctgggat aacaccaacc ttactggtag ttaagccaat tggtggagga ggaagatttt
    18901 acctcttggg taataatgga acttttccga tacagggaat aagtattagc gcaacgggaa
    18961 tagtgggtga cgttgagact ggggtgaaac gtaaaattaa tgtaattc
//
LOCUS       DDWP01000044            9473 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_35204, whole genome shotgun sequence.
ACCESSION   DDWP01000044 DDWP01000000
VERSION     DDWP01000044.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_35204"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2924 a         2028 c         1561 g         2960 t
ORIGIN      
        1 agctattctc gagttagaaa ccacactaaa atcatattta gctatcaaca aaatttctcg
       61 tatcgaatgt tacgacatct ctcaaatggg taccaagcac tttgttggcg ctatgacagt
      121 ttggcaggac ggccacatcg ccaaaaatga atatcgaaaa tttaagataa attccaaagt
      181 caccccagac gatcaattta tgatcaaaga agtcgtctac cgtcgtctcc agcatccaga
      241 gtggcagttc cctgacttaa ttgtcgtcga cggcggtaag ccacaagtgg ctgcagctaa
      301 agttgcagct gcagccatcc tgagcgaaca tagtgagtcg aaggatattt ccattatcgg
      361 tcttgccaaa aaatttgaaa ctattgttat caaatctgat gatgatttca ttgagattaa
      421 tttacctaaa aattctaatg ctctcaggct cttatgcgcc ttacgagacg aagcccatcg
      481 ttttgccaat agatatcgtc gcgagcttat ggcaaaatcg atctcatgaa tgtcactgct
      541 atagtgcctt gctacaacga agaaaaccgg attctgcccg taattaatgc tattaaaaaa
      601 tcaaaactaa tttcagaaat aatcatcgtt gatgacggtt ctaataacag ttccaaacaa
      661 gtactaaaaa agataaaagg tataaaaatc ctaacccacc cacgaaatct tggtaaatca
      721 aaagcactta aaactggcct tttatctgct caaaataaat acgtattttt tattgattct
      781 gacttagttg gactaaagcc aaaacatgtt gacagaatga ttgaccagtt tattaagcac
      841 aaactaacga tgattcttag tgagcgtgag ctagaacatt ccttcaatcg gtttaatggt
      901 tttgctattg cctatacggg agaaagaatt ctgcctcgtg attttcttat tcaaaatatt
      961 tcagttttcg actatccagg ttatcttatt gagccagcaa ttaatcgact agttttttca
     1021 aaatataagg ccggaaaaat cgttttaaat aatgtcggtc aagtttttaa aattcaaaag
     1081 ggtggtttaa aacattggat aaacgattac aaaatggtca ttgattatat aaaatttctt
     1141 gggccaaaag actttctcag tcagttagtt tttgcaaaaa agctatctga ctattcttaa
     1201 cataactaca tttgccaata gatatcgtcg cgagcttatg gcaaaatcaa tctcatgaca
     1261 gtatcagtca ttttgccatg ttacaacgaa aaacgtagaa ttctccctat tatcaaggaa
     1321 cttaaaaagt ccaaattagt cagtgaaata attgtagttg atgacggttc tgaagttgaa
     1381 acaaaaaata ttcttaaaaa gattaatggt ataaccctaa ttacccaccc aaaaaatgcc
     1441 ggaaagtctc aagccatgaa aacgggacta cttcactcaa aaggagacat tatcgcttat
     1501 cttgattcag atcttcagca ttttactagt atgcatatcg acgatctagt taaaccaata
     1561 atcaataatg actgtgatct aacattaagt gatcgcacca aagacctcta tattcttaga
     1621 ggtttttcgg ccgcctttac tggcgaaagg gccgcaaaac gtgaactatt tactaaaaac
     1681 ctttctattt ttaaagccaa aggttttctc attgaggcat caatgaacaa aaagtttttt
     1741 aaaaataaaa agataaaaat tcaacgtgtt acgtttagga acgtctgcag ttaccttaag
     1801 gtcaagaagc ttggtataaa aggtcaaatt caagatatta atatgttttc agatatggtt
     1861 aagtatttag gtattaaaga aatggctcac caggtttata ccgccatgat gttaccgttt
     1921 gctaagtagt catgggtgta aaagtcagta ttatcacctg tactaggaat tcagataaat
     1981 acctttggga gtgtttacat tcagtagaga atcaaacata taagaacatt gaacatatta
     2041 ttatcgatgg ttattctaat gacagaacta ctacgataat caaacaatat attaaacgtc
     2101 accacgatta tgacgtaagt tatcatactt atcctgctcg tggtattgcc aacgctttga
     2161 atattgggat caaacattca actggtcaaa taattcattt cctacattca gatgattatt
     2221 attattcatc aaattcaatc actaaagtta ttaattactt taagcaaaat tcccaatata
     2281 attggatact aggcaatcac ttgctcgcaa tttgtggcag aacattgata ttaccaaata
     2341 catttcttac aaaagttatt ggtaaacact ttcttaaaat attcccttgg atgtctcatc
     2401 aaaacacttt tatgagacgt attgtctatg atacttatgg tttattcgat gaaacttacc
     2461 agaataattt agattacgac cagtggctaa gaattgtcaa tcgtgagcca gtattatttg
     2521 tcaatgacaa ttttagtgtc tttcgagtcc ataaaaagag tactacattt aatccaaaaa
     2581 attaccaacg atggtttaga gaatatatca aagcacaccg acaaaatcct agctaacccc
     2641 caacatctct ttcattctca ttaatccctc ttttgccgga ctttgttcaa agtgttctcg
     2701 agcaggtgcg agcttggcaa tgatccattc agctaaagca ttctttagat caagtggaaa
     2761 taattctcca ctcccataat cagcttttaa atcagttacc ttagtataaa ttttgtcacc
     2821 accaaacttt tctggtcgag aaattttcaa ttcgcctatt tcttcacccc agaacactaa
     2881 tgtctctacc caattaatta atggattaaa ttcaatttcc tttggtggac cgtaagcttt
     2941 tttgatttta tctctaattt catctggggt atcatgaata aaaattgctg atccaggttt
     3001 tgatttactc atcttcaatg tctccgcatc attttgacta gtatctggac cagtcaatcc
     3061 ggcaatcaaa ttttgatgaa tggctattgg tgtccaccct cccggtagtt tgtcacccac
     3121 ttctcgagca atcacgtgag ctttacgttg gtcaattcct gcatgagcca agttaactcc
     3181 catggtaaat atatctgcca cctgaagtgg tgggtaaaac aatgttgcca tgtctacttc
     3241 agtcccttgt ttcttaccca taatcgacac tgatcttaaa ttacgagaca gtgttacatg
     3301 tttgcccact cgcataaaag tttcccagtg tgttgggtta ttttcgtata attctttacc
     3361 gctaataaac tccacttcat tttcatcgac tccaaaacac tttaaagaag caattaatcc
     3421 ctgtttaaag taattatttg tcgcttcccg aatattctcc caatttcctc ccattttatt
     3481 atttaaaaaa gaatgaaaat cagccaaaaa tatcttgcac tttactccag cctttagaaa
     3541 atcagcaatc tttcccatag atattagtcc agttcccaag tgcaccaatc ctgatatttc
     3601 aaaaccgatg tagtgattaa tagttgtccc cgattcaatt agcttcttta aatcttcttc
     3661 tgtcaacacc tcctgacagt tacgagtaat taattttatt ttttcttctg ttgtcatagt
     3721 gtgaattata aactaaataa ataataaatc tccttctttc gccacatgga aaccagcatc
     3781 cacaaccatt ttgaattcaa cactgttttc ttgtaatact ggatttgtat gatttaaatg
     3841 aataaaactc accttatctg caaatggcaa cagttttttc aaactagtgg atatggttgg
     3901 atgtggaact ttagttatat ctcgacccgg cagttcattc ccattaaaga aagtaccatc
     3961 aacaaaagcg tggtcgactt ctaataatat attttcaatt tcattaaatt catcccaact
     4021 atctatatcg ggtaagaaca gtatcttttt atttggccct tggataacaa acccaactgt
     4081 ttccgagaat tcgtctcgat gtggaaccaa gactggagta aatagtactg agttacttac
     4141 tgcgacagga actttattac ttaatttaat caagtcaatg ttttctttat ccaccaacga
     4201 cctccacggc aaattattcc taataagcgt gtctagtttt ggcattgccc aaaccttgag
     4261 caagtgactg ttcataacag ctttttccaa attaattagt ccagtaaaat gtccaatatg
     4321 cccatgtgtt agaaaaagac catcaatatc attatttttc tgacactcac ttaacatctg
     4381 ccattgatct ttaaaatcgg gagtagcatc gatcatccac gatttgccag cttgctgatc
     4441 aactattcca atcgacgcca ccattcgcct gtctttaggt gatttccatt ttgacttaca
     4501 acacttttta acacaaccac actgtggtat tcctgcatct tgggcaattc ctagtatttt
     4561 tataaaagca gtatttttca tattatttta attctccaat ccataatggt actgtcatgc
     4621 attctcgaga ttggtgcaaa tgtccccaaa ctttttgtcc ctcaaacata tcgccaaagt
     4681 tctcgccatg gtcaccatga gcaatgacaa ttgtcggtcg atcaccaccc cgtatcttga
     4741 ctgcttcgag cagactacca actcgcgtgt cgaaaatttc tagcgcatcg acttgcatct
     4801 gtctaaaccg ttcagcaaaa ggctcaagaa tttcctttgc cctcattgta tctgggtcag
     4861 ttctggttga tcttaaattt agtactttgc ctaatgttgg caatactttt tgtatatcat
     4921 ctggtattcc taggccagag tcgtaacagt aatgtgtttc ctgagcgttc agagacaaca
     4981 aaaatggacc agttctcggt atccgcttca tgatttcatc aacatgggtt aatgggaact
     5041 ccttcggctc tctgggtttc atttgttcct catttagatt tggaccaaaa tatatagagt
     5101 cactccacgg aaaaccagtt cgcaaccaac ttccatcaga aaattgtgaa actccaccaa
     5161 cacattgaac atgaaaatca attttcctaa acccgtctat cacactcgtt ccggctaaca
     5221 atatgccgca gcctttatta gcatctcggt caggtgcagt aacgattcta aacatttgtt
     5281 tggttggctc atggtaaaaa ggtaaattgt cgccacctcg cgttgaggga aagtgaccag
     5341 cccacattgc tgcatgtgcc gccggagtgt atggtgccaa tgtccaagct tctcgctttg
     5401 atccaattgc atccaaattt ggagtccgcg ctctctgcgc gcaatcccaa gaacaactat
     5461 ccggtaccaa ccagacaata ttggcatctt ttattggcgg caagttctca agtcgttttc
     5521 tttcagtttc aaaatcaatc ataaatagtt taactaataa aaacaattaa taaaaattgt
     5581 tttgtccaac gggcaagact tgcacttgct taaaaaggtt ttacagacct tcgcctagct
     5641 attcggccac cgttggttgt ggcacaattg agacgtctct cgactgtgcc tatctatagc
     5701 acataccatc aacccctcag atttaaacca ctaaatacac ggaccatgtc caattccggt
     5761 tttagctccc ttggtttagg taaatctggc attttctttt ccttaatagt cacttcagct
     5821 tttcttacgc gttcagcatt acctctgcca actgaagttt gcattattga gtcacacata
     5881 ttttttccac ctcctttttt taattaaata attaataacc accaaaaagc ccccacgcct
     5941 gtaaacatct aaaaagacgc ttacaagggt gagggcttct cacggtacca ccttgttttg
     6001 taaataaaaa aagccccttt ggggctcgat aattatttac ctcattatcg agtattcgct
     6061 tacgcggtct ttactctagc tctattccgg gagctcccgt actgattttg tcagtatgct
     6121 cagagagtgt attatgttct ccttaacgcg ttccaaactt aaaaactatt ttatattaaa
     6181 catttaaaat agtcaacagt tagattatag gcttatattt taccctcctt tttgaaggga
     6241 gggtctccgg tgaataccgg aggggtgggt ttaaactctc tcagcaaaca ctagcaacct
     6301 tttccaatca gttcctattg gccaacaggt ctgaagtatc actacttctt tttctggatc
     6361 cgagtattcc aagaattcta cctggttagc atcaaccact ttctgctgat ataccttata
     6421 catatatcca actccatctt tttgtagcga cacattatcc ccatttttta actgtccaag
     6481 tagataaaac actgagttat ttctaaccac tccaaatcct tgctgagtcg aatgagcaaa
     6541 tatgtatatt gtttttcccg ccccgctacc cggggcatca gtgttttttg cctgagccac
     6601 tagatcattc tccagtactc tcaaatattc ctgactattg aagggtgata cgttttctac
     6661 cacctcagaa aacgctaata tctttggaat cgtaattgta taaatatcag cattttctgg
     6721 cttttctacc tcaactacca ctccttgctc taaagaggca gttgtctttg acctctcata
     6781 catgaataat gccttaccta gtggataata taaataaagc aaataaagac tggccaatat
     6841 taaagatata tttgctatgt aaaaaacaat atgagcaact tggctagggt tatgatatat
     6901 taccccactc tttccgacta ttttcacctt tctctttttc ggaaccactc ttaaaaaatc
     6961 tattaacatt tgactctttt taaaaagtta aattaatttg acaaataaaa atgcataaac
     7021 taatataccc cctatgacaa taaattgctt cacctgtagt gaccgccatt ttattgtcaa
     7081 acctatttgc ctttcctaaa cacttctgaa ttgctcaaca atcagaagta gctaaagaga
     7141 agggcttttt ttgtcagttt attaaaataa aaaatgaata aaacactctt gacccagtcc
     7201 ggttacgaca agctatctgc cgagatctta accatgaaag agaggcaaaa tcggctaatt
     7261 actgagattg aagaggtcgc ccaaccagac gagtctggtg aagacggtct agctactcag
     7321 ctcaaagaag aacttgaagt tcttaacgcc aaaataaccg agatcgaaga ggcacttgaa
     7381 gtcgcccaga taattactga taaaccttct caaaaaacca ctattgtcgt tggctcaact
     7441 gtaaaagtta agatatctag caagagcgaa aaacaatttt caattgtcag tcacctcgag
     7501 tctgatccta gccaagacaa aatttctgac caatctcctt taggtcaagc tttgattggt
     7561 aaaaaattgc atgacgaagt cgaagtcgat gccccaattg gaaaaattac ctacaagatt
     7621 gtctcaatca actgatggta aaatagtcca tgtccgacat acttaccaac caacgtcaag
     7681 ataaactcaa gaaaattcgt gaattaggcc ttgatgctta tcctcaacca aatctagtta
     7741 accgtcaatc aacctcagag gctcttgaga tggttgacca acaggttacc gttgctggtc
     7801 gtgttctatt actccgtggt catggtaaga ttctctttgc tgatttacac gatatatcag
     7861 gcaaaatcca acttttcttt gagaaagaca ccttaaccga aaaaatggca ctaaccaaac
     7921 tccttgatct tggagacatc atcacagcca gtggacaagt ctttaagact acttcaggtg
     7981 agaccaccat ccgtgtttct gattttcaaa tacttaccaa aaatcttcga cctttaccag
     8041 aaaagtttca tggtcttacc gataccgaag aacgctatcg tcaacgctat gttgatttga
     8101 ttaccaaccc cgaatctcgt gctgtttttc tgactcgcac caagattctt accaccatgc
     8161 gtcaattcct cgatgaccat ggttttctcg aagttgaaac tccaattctt caaccggtct
     8221 acggtggtgc ttctgccaaa ccttttgtca ctcatcataa tgaactcgac caagacatgt
     8281 atcttcgtat tagtgacgaa ctttatttaa aacgcctaat tgtcggtggt tttgaaaaag
     8341 tttacgaagt ctctcgtgat ttcagaaacg aaggtgtttc tcgctttcac aatcctgagt
     8401 ttactcaaat cgaattctac tgggcctatg ccgattatga cgaattaatg cgctttactg
     8461 aacaattagt ttctcatgtt attaaaacag tcaaaggtga tcttaaagtc aaatttcaaa
     8521 atcaagaatt agattttacc ccaccttttg ccagactaac tttccgtgat gcaattcttc
     8581 aaaaaactaa tatcgacatt gatactatta agaccgaagc tgaattccac cagaaaataa
     8641 aagatagcaa ctttaaatta gatactaatg gagttgttgg actaggtgca ctttttgact
     8701 ctttatacaa agaatatgtt cgaccatttt tagttggtcc agtctatatt accgactatc
     8761 ctgccgagat gatcgccctt gccaagagga aagctgataa tccggagaag attgcttcat
     8821 ttcaacttct agccgctggt gctgagctcc tcaaagctta taacgagtta aatgacccca
     8881 aagatcaaca cgaccgttgg ctcgatgaag ttgctctaga aaagaaaggg gctgaaaacg
     8941 ccatgcaaat ggatgatgat tatattcgtg cccttgaata tggtatgcct cccactgccg
     9001 gttggggtat gggtatcgac cgttttaccc aatttatcac cgactctccc acaattaagg
     9061 atgttatttt gtttccatca atgagagaag agaaaactac acaaaaatct tctccgattg
     9121 gtcttcaaaa tgataaagtt gttgtcggaa aaattattga atttgaaaat catcctaatg
     9181 ccgacaaact gcatgttctt aaagttgacg tcggtaatga gcaacttcaa atagtttgcg
     9241 gtgccgataa catcaaggtc ggcgacattg tccccgtctc tttagttggt gctcaagttt
     9301 ttgacgagga caaagaagcc atggacatca aagtagccaa acttcgcgga gttgaatcaa
     9361 atggcatgct ctgttctgtt cgcgagcttg gcctcggcac agaccacaac ggtattcatc
     9421 ttttagatga aaagttatcc gaaaacctcg gccaacccgt caaagatcat tta
//
LOCUS       DDWP01000045            8634 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_8852, whole genome shotgun sequence.
ACCESSION   DDWP01000045 DDWP01000000
VERSION     DDWP01000045.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_8852"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2428 a         1359 c         2008 g         2839 t
ORIGIN      
        1 tgtgtttttt aaacgatgat aaagggtaag gaaggtttct tctactgtga acaataacca
       61 aaggatattg cctaatagtg gtcctaagtg ttttcgaaaa tatattttcc tagacttgta
      121 ccagtgtttt aaaacaattt tatatttgct attactacta tgtcccccaa catgggttat
      181 agacgactca ggatcaaacc atgtttctag atcattagat tttataatgc taccaaaatc
      241 cacgtcttcc atgtacaaga agtagtcttc acaaaacctt acttttggtt ttttcctgat
      301 aaacatgtat gccccacaca gagaatgcac tttaatgggt gtttttatgt tttgtgtttt
      361 ctctatccaa aatcttttgg aatagatatt ctcaggaacg atttttttta agtttgtgaa
      421 ctcaaagaga gcagtaagaa atcctggctt actggtcgct gtgtacctat cctgaagagt
      481 ttctgggttt aagattcttc ctcctatggt accaatgttt ttgtttttta ttatcttaga
      541 ataagatttt aatgggcttt tgtctcttag ttcacagtcc gggtttatca atagaattat
      601 atcggttttt gattcgtcaa ttccttgatt cacggctttt gcaaatcctg tattattact
      661 gttcttgata aatcttacct ttttatttga tgtagaaaaa tgtttagtgt cgcttgaatt
      721 gttgtctatt actatgatct cgtatatctg ttcatttatg ttatcgaggg atttgattag
      781 acgtttaagt ttttctgtgc tgttgtagtt tatggtaaca acgcttattt tttcttttat
      841 tgttgacata tatttaaaac aagaatatct taagttgtga gaaaaataac tttgtttttt
      901 tcttgggttt gtttttgttt ttattttctt gcttcatcgt caccgattaa tgcaagttac
      961 ttattttacg acgatttttc atctggaggt attgataaat ggactgtagt tggtgaagat
     1021 ttctggaaag tgcttggcga agaaaagaca ttatatagtg aattgtctta tagagaacaa
     1081 ggctatctat tggctaaaaa cacttataat ttaagtgatt ataaaatgtc tgtggaggtt
     1141 agaaatgtgt ctggtattga tcaacatttt gtgtttagga cgaaggaaga tcttagtgct
     1201 ttttatcaag tcgagttgag atttcctgat ccaggttggc cacaggatcc tgcttcaaat
     1261 ctaaggttgt ggagatataa agatggaaga tatgtaagat tgttgccaga tataccatac
     1321 agtctcgtaa aagataaagt tcataactta gttattgaac tggagggctt taatataaag
     1381 atctatgtgg atggtaactt ggtaatagat tttatcgacg aattagatgg gatatctgag
     1441 ggtggagtcg gtatccatgc gtatgctggt gattatcgtc ctgctactat tgtcaattat
     1501 tatgataatt tttctgtatc tggccttgag tcaactcctc gcaataaggt tgtcgtggtg
     1561 ccgggatttg ggggttcgtg gaacaccgaa gcactagttt acaaccgttc ggtcactgat
     1621 gattcttggg tcatgactcc gtttgtaaaa aattatgacg ggctaattaa tactttgatt
     1681 gaacaggggt atgaacgtga taaagatctg tttgtttgga actatgattg gaggaagcct
     1741 gtcgcagata acgttattaa atttaattcg tttgttgggt caaaactcac tgatacgcaa
     1801 aaggtcgacg tggtgggtca tagtatgggt ggtctgattg ctagaatatg ggcgcaggac
     1861 aatattgata gtggtaaggt tggtaaagtg gttactctag gttctcctca ttctgggttg
     1921 gttaaagcct atgagtggtg gaatggtggt aggatttcgg gcgataatga tgtaggatct
     1981 attgctttag cgattctcta tcagctaaat cgtcgtaatt tacagagtga ggtggaagtg
     2041 ttaagaaatt atgctccttc tttaaaagat gtcttgccga cttttgattt tgtcaaaaaa
     2101 ggaagtaagt taatctctgg gaatgatttg tattttaaaa atgattacct taaaggcaag
     2161 aattcttcag ttaatctgat atttgataat ttgttgactg tggccggtat tggtcaaaag
     2221 acaacgagta atttagtttt gggggagaga aatgtttttg acaaagttct caataaatgg
     2281 ccagatgggt caccgttgaa atatgtttat gctgatggtg atggagcggt tttgaaaaac
     2341 agcgctgtat ttagtggaga tgtctatgga gaaaagatgt ctgatcatgg ccagataaca
     2401 gataaggcaa ttaataccgt cttgtctgag ctaggaatcg gggtaagcag tactcagcca
     2461 atttatgaga tgagtaagaa ggcagttttc tttattggtt caccggctta tttgaatgtc
     2521 aaatgtggca ctgactcccc tatcgtttct gatagtctag ggtttgtttt aactgaaaat
     2581 ttgggggatt gctcagtaaa tgtagttggg actgggagtg gtgtttacca tttagttact
     2641 ggtacgactt atgatgataa ttggcgttat tttgaagata gtataaatgt aggggaaact
     2701 agggtttttt tgataaataa tggaaagtta aaaagtgatg tcgtgaattt agattcttta
     2761 tacaggttgg ttaaatatga tttggtgaag ttgcaatcgc aatatcctgg taatactaac
     2821 ctgacaacgt cgatgatggc tgcaaatgca aaacaagtaa atagtttaat gacgggagtg
     2881 tttagtttta gaaaggagaa aaaagagagc ttgataagtg gggcaattat gaagaacatt
     2941 gagacaatac tggcgataga aagtcgtaat ctatcgccca ctgtggttaa agcggcttat
     3001 gtaaaagcag tgcagagcaa gtctttggtc gacaggatga ctaggctgta ttctacaaag
     3061 aaggttcagc cttcggtgtt tggagcggag agttataagc ttttggaaaa gatggtggat
     3121 cagagtggta gtgactatgc taagtcagta ttggcagtaa agattgcgtc agaagtgtgg
     3181 agatgaatat tgtataattt gacattggct gcttggctca gtggtagagc gttccattca
     3241 cattggaaag gtcagtggtt cgatcccact agtggccact atgaaaatag gaaattttat
     3301 actggagttt gtccagtcaa tagttctagc tttatcggta tttgtgttgc tctacctttt
     3361 tgtggctcag cccaacgagg ttaagggtag ctcgatgatg ccaaattttg tagacaaaga
     3421 atatttattg acggataagc tgagttatca atttggtgat cctcaacgag gagatgtggt
     3481 ggtttttaaa gcgccaccaa gtgaaccttg tgcagtagac gagtgtgaat atatcaagag
     3541 agttattggt gttcccgggg acagggtaat ggttgaaggt ggtaaggtgt atttaaatgg
     3601 atctatgttg aacgaatttt atttacctga agattttgtg tctgatcctg gtgattttct
     3661 ggaagaagga gttgaacaag aagtgccaat gggtcattat ctatgtttag gagataacag
     3721 gtcacattct cgtgatggac gagagtttgg gccaattgaa aaaagattga ttgttggtaa
     3781 agctttcttt atatattggc caactaattc aataggatta gtcccgcaag ttagattcta
     3841 gtcgttgtgg gtgaattctt tgtggcagat gtctttatat agttttttaa ggttacgaaa
     3901 acggtggtct actggtcctt taaactggaa ggttactcga gttagaacgt tggtttgagt
     3961 aatctttact tttgcggtat tgtccggaaa aagtgattct atatcgttgg aaattgtgtc
     4021 taattttgat tttagaaatt ggggtaagtt ttctttggta ataggtacat ccccttcttc
     4081 aatttttttg cgcattcttc tggcgatttc ttcagctctt ctaacagaag ctttttcttt
     4141 gaggacgatt ttgtaggctt caatgataag acgagtgtca ccgatactag ataaagctcg
     4201 agcatgacct tcgctgataa gtccagaaag taacccgtct ttgagagcgt cgggtaaggt
     4261 gagtaatttg agtatattga taacgtaggg aacgctttta ctaatttttt tggcgatgcc
     4321 agcatggtcg agattaaatt cttctttgag acgaagaaat gacttggctt tttcaagtgg
     4381 atttaagtct ttgcgttgga cattttcgac aatggccatc tctagcattt gctgtgggga
     4441 ggtctttttg ataatggcgg ggacataagg tagctttagt gtcctggcgg ccctccaacg
     4501 acgttctccg gcgatgattt gatagccggc gggtgtctga gcgacaatga gtggttcgag
     4561 tatgccatgc tctctgattg actcgactaa ttcagagact gattcggggc taatgactcc
     4621 tcgaggttga agaggatttg gttcgagttg gttggtgggt atctgagtaa tatctttcat
     4681 agtaaaagat tactatgaaa actcctgtga aaaaagtagg acgaactagt tcaaatgtgt
     4741 gatatactat tataggttat gaaattaaaa gatattccat caggaacagt aattaagttg
     4801 attgggtcac ctactttggg gcctgttatt gttgataaaa ccatagaccc tgagggaaca
     4861 caaattaggc tcaagtggcc aggaacgttt gaagagtgtt ctggcagttt tccgggtgat
     4921 caagatgtac aaatagttaa tcgagataaa gcagatttaa gtggagtttg gccaagaaag
     4981 aaaacagctt aggcgtggac gacggagacg aattttttac ctaacttttt gaagaatttg
     5041 actattcctg gcttggaagc gtagacagtg aaatctttgc cagttttggt acctggaccg
     5101 ttgtggaaat gggaaccaac ttgtcgaacg atgatttgac caacttcaac tttttggtcg
     5161 ccaaatttct taacaccccg tctttgcccg ggacgatttg acttctgatt ggtttttcct
     5221 tgtgatttgg tatgtgccat ggttagttat aaagttaaaa gttaatttta gtaattttaa
     5281 tgtcggttaa ttgtggtcga aaaccagcag aacgtcggta tcgtgatttt gatttgaact
     5341 taaagacgtc tattttttcg cctttatagt ctttaacggc ttcatattct atagaagcat
     5401 cttttacaaa aggtgttccg acagaaactt tgtcttcgtt aactgtgaga aggacatcgt
     5461 taagagtatt ttttttacct gtttccaaac tttgattgtc gacagtgata acttgtcctt
     5521 cactaacttg gtattgagtg ccagatatgg caattacagc gtatttcata ctggggaatt
     5581 ataacagaaa aatagtaatt agtaattggt aatatgctaa cttgaggaag atactcctaa
     5641 ggagaacatg agagccataa tggacgatcc tccaacagac atgaatggta gaggtatgcc
     5701 agtaacaggt atcattccaa ggttcacgcc aatattgata aaactttgtc cccaaacttg
     5761 aaaagctata cctaggataa agagagatag tggccggtta ttacgtactt caaatgccct
     5821 gtttattaat gttttggtga tgaggtagta agagaagata atgagagaaa ctccaactag
     5881 ccccagctct tctgccgtga cagcaaacat aaagtcagta tgttttgcgg gaagaaacaa
     5941 gagttgtccc tgactcccct ttttgtaacc ttttccccat aaaccgccgg aaccaatggc
     6001 tatttttgac tgaataatat tgtatccatg gccttgagga tctgattgtg ggtttaagaa
     6061 ggtgactatt ctttgtcttt gatagccatg caaaacatga ctccagatta ggggtgagga
     6121 cattatcaaa agtaaggcgc ccaataaggc taatttaaaa atttgtttgt tgtagagaat
     6181 tatgggaact aacaagagcc caatggtaat agcactcccg aggtctggtt gaagcataac
     6241 tatacctata ggtatgaata tgagtagagg aaagttggtg tggacaagaa ataacatcag
     6301 ccaaggcttg acgaattcgg atggttggaa tgatataaaa ccgagattta tccaacttct
     6361 agcaccacgg gttatgttgt tgagtagaat tggtaatagt aaaaaaaaac aagaagtaag
     6421 gaaaatatac acttttgaag aagcgatttg gcgagggtta atttgagtgc cgataaaaaa
     6481 caaaatcact ccgagaagcc aggcgaagag ctgtttccct agtgcgtatg gggcgactga
     6541 actgaccata agcatgttaa agcagaagag tatgccgaga ccaatagcaa ttggtggtaa
     6601 ataattcttg gtcatgaact attctctctc aattctggtt tcttcgcttt ggttaattga
     6661 gatagctaaa gtcttttctt tgatttgtac ctcaaaggat tttggccaag atggagcaaa
     6721 tatttttatt ttatcttgaa cttgcatacc tgactctttt ctcataactt gtatgttacg
     6781 gataaaatca cgatattctc cctcttcgac tagctcagga gttagattgg tgtcgagaga
     6841 tacttctgta ccttgtttga cttctttaac gttggtttct tgagcaatga tttctttaag
     6901 gtcattgtca aagttagtaa taaccgtggc tgaagctagt ggctgtctaa cttttagaga
     6961 tgatttttgt ctaagggcgt gtatctcttg acaggttgct cggacaaact ccatctgggt
     7021 ttcgagattt ttgtcaataa gctcctggtc aaaagatggc cagtcttgga gatggacaga
     7081 gccagcagtt gagttgtgaa tattttgata gagagtttca gccaagaaag gtgtgaaagg
     7141 ggcgatcaat ttagttaagg tgtcaaaaca ataaaagaga gtgtcaaaat tatcactacg
     7201 atcgcggctg cggcggatat accaagttga tagatcgttg acgaaacctt caatagtttg
     7261 ggtcactttt gaggtgttgt acttatctag atttttggtg gctgtctgaa tagttgaatt
     7321 gagtcgagag ataatccact tatcaagaat attggtagat ggggcgtaag agctaggtgt
     7381 ccaaccatcc aaatttgcat gttgagtgaa gaaacgataa gaattccaca aggttaggaa
     7441 gaattcacgc ttaactttgt cggcaattgt atagccgaaa ctaacattgg tatagggctt
     7501 ttgttggcaa tagagccagc gcatagagtc ggcacccatt ttttcaacag catcatcgta
     7561 gggaatacca ttttttttgg ttttgctcat tcgctcacct ttctcgtctc ggacttcggc
     7621 gtgggcgacg acatttctat aaggaaaggt ttcttccaag gtaacactga agaaaagcat
     7681 agagtagaac catagacgga cctgttcgat catttctagg acaagctcgg ccgggaacca
     7741 cttgctccaa tattttttgt cgctaaggta ttttagagta gagaacggga caactccggc
     7801 gtctagccag caatcaccaa cgtcagggac acgtttaacc tcattctgac attcgggaca
     7861 ttttatgagt atttgatcga tccatggtcg atgtaaagat ggcattttgt cgactaaaga
     7921 tggatcaaca gcatgctctt ttaattcgtc tttactgcca atgatggtga ggtgtccaca
     7981 agagcattca tagaaaggta gggcgaggcc ataaaacctc ttgcgactaa tcatccagtc
     8041 ttccattgag tcgagccagt tttgcatgcg tcgaccaaca tattttggat gccactgtac
     8101 aagcttagct gcctctttca tttttggacg tatctcatct gagttgatat accaactatc
     8161 ttcgaggcgg aagagacatt tagtcttaca gcgccaacaa tgggggtaac gatgggtaat
     8221 ttgttctttt ttgaataaag cgccagtttt ttctagataa gtcaggactg taccaacaac
     8281 atcatgagca tatagacccg atagttctcc ataaccttcc ataaaatgtc cagtctcgtc
     8341 tagtggagca atcatgtctg ttccaagttc tttacccaat tcaaaatctt cttgaccaca
     8401 tccaggagcg atatggacta caccagttcc ttcttctggg ctaactagct cccaattggt
     8461 tatatagtgt ttgacgtttt cttgagcggg aatgttatat agtgattcgt attctagatt
     8521 tagaagttct ttggcatcga tcttttggta atctgttagt ccgagccggt cggcagaaag
     8581 ttgggctaga taaatggtct gattttcatg tttggctttg acgtattgga actc
//
LOCUS       DDWP01000046            5550 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_8850, whole genome shotgun sequence.
ACCESSION   DDWP01000046 DDWP01000000
VERSION     DDWP01000046.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_8850"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1802 a         1327 c          879 g         1542 t
ORIGIN      
        1 aatcttttaa ctcaaccaac aaatcaacct caacaaatca ttgttgaccc agttgttact
       61 atcgctccat cagctagcga cacaccagct attactcaat aaactttata actttacaaa
      121 ctttataaac taatccatgc caaacaacgc ttcaaaaacc aaaaagaaag agttcttatt
      181 ccaactttcc tctgttactc ctccttttga tgctgcttgg tacgttatca acacctattc
      241 cggtcacgaa tacaaagtca tcgatgccct caagactcgt atccgcacca tgaatttgga
      301 agatcaaatc ttccaagcta tcgtcccgat tcaatccaag atgattatcc gtcgcggaac
      361 taaggtcaaa actcaagaaa aaacctatcc tggttatgtc attatccaaa tgcttcttac
      421 cgacgaagct tgggtcactg tccgcactac tcaaggtgtt accaatttta ttggtatcga
      481 caacaaacct tctgccattt ctcaggctga agttgatcaa attcttaaaa ccaccgaaga
      541 agacaagcca aaatatcaag caccatacag cgttggtgat gttgttaaag ttattaatgg
      601 acctttcgcc gactttattg gttctatcga ttccatcgat caagaacgcg gcaaagttcg
      661 tgtccttgtc tctttcttcg agagagaaac ccccgtcgaa ctcgactttt tacaagttag
      721 ccgagaatta taaaaacttt ggcaagcttc agccaaaatt taattaaaat ttatctaaag
      781 ctctaacgct ctagcgttct agcgaactat ctttatggca aaaaaaatta aaactatcgt
      841 caaattagcc atcatggctg gaacagctaa tcccgctcct ccagttggta ccgctcttgg
      901 tcctcaaggt atcaaaatca tggatttctg taacgaattc aacgacagaa ccaaagacat
      961 gcaaggttct cttattcctt ctgtcatcac tatctacgat gatcgttctt ttgatttcat
     1021 tatcaaaaaa cctccagttt ctgacatgat caagaaaatt accggaattt ctaaaggatc
     1081 aagtactacc ggaagagaca aaataggtaa gatcaccaat gctcaaatcg aaaaagtcgc
     1141 caccgacaaa ttggtcgact taaataccag tgatattgaa gctgccaagc gtatggtcgc
     1201 cggcaccgcc aaatcaatgg gctttgagat tgttgagtaa acaaaatgct tcccatatta
     1261 cgatcacgag aaagtataag cccaaaacta cccgaagctg atcctggccg caccaacgaa
     1321 atagtcatgt gttcacgtat tcaaagtcta gttatctgcg accatgcagt aattgaaaaa
     1381 attggtgaac cttctcttcg tggtacctca ggatatcaag tccaagaagt caggattgtc
     1441 gtcagaaaag gagatggtac cacttgtgct acctgccgaa tgccaaaatt cagataatta
     1501 cttaaatacc ggatacacat ctgaaacaca cactttttcc ataaaagtag tacaataggc
     1561 agtaagttat caataaactt acctcatgtc attagcccag agaatcgagc aagctcagtc
     1621 agcaacgctc atgtggccat caccaacttc actaccagta gaagagaatt acaaacaact
     1681 ctctccatgt ggaaaaacac tctgtgtaca gccttcatgt gttgcattct attgtccgga
     1741 accaccttca acaagatgtt ctacatgtca tcaaaagatc gccgacaact atggcaatct
     1801 accaggcacc tattaagtac actccatctg tcactttata cgctataatc aaaagattat
     1861 tattttcata aatctaaatc tatgagcaaa acctcacaag caaacaaaat tgcgaaggaa
     1921 gagtccaaaa agactcgcaa tgccgaagtc tccactgaag ctgttacaga agaagtagtt
     1981 gaaactacct ctgaagcagt agtcgaagaa gtatccacta ccaaaagaac tgcaaaagtt
     2041 cgtggtaaag cctatcaaaa agtccgtaag atggtcgacc ccaacaaact ttatagtctc
     2101 aaagaagctg tcaaattagt taaagaagtc tctttatcca aattcgaagg taaagtcgaa
     2161 gcccatgtca ctattcttga catcggtaat gctggcgaga ttgtcttccc tcatcttgaa
     2221 gcttccagca aaaaaattgt tatcttaaac gatgctattt taactgaaat caaagacggc
     2281 aaagttaact tcgacatcat tatcgctact ccagttacca tgcctaagct tttacctttt
     2341 gccaaacttt taggtccaaa aggtttaatg cctaacccaa aaaatggcac cttaactgac
     2401 aagccagaag aagctgtcaa aaaactttct gtcgccaaga cagtcattaa aaccgagaaa
     2461 aaagctccag ttgttcacat catcgttggt aaagttagcc aagatgctaa agaaattgaa
     2521 gccaatgtcg aggaattaat taaagtgatt aaagctcaaa agatcaaaaa acttgctctc
     2581 tgtcccacca tgggtccgag tgtcaaagtt ttgattgaaa aataatttat ttttcgaaca
     2641 ccaaccccgt cggtgtgacg tagtcacata atgacggggt tttatggtaa attaacccat
     2701 gtcttcccga tctagtaatc acattcatta tcccctccct atctttttta aagatatctg
     2761 gagctataca gccggatttc accgcagaat agctatgtgg agctctcttc atattgtctc
     2821 tgccattgct aacctcctcc ctcccctgct ctttgccaag atagttgatg atatctctaa
     2881 caatcagacc gccaatttaa ccccgctcgt ttttacaatt tttttaatta gctatggacg
     2941 aattgttggt cgcaatcgta tcaaacttca aatctacaaa ctcgccgaag cctgccgact
     3001 caatagtcgt caaagatgga tatccactat cattaaatac gatcttaaat ggcatgagaa
     3061 acaaagttct ggcaaaaaga tctctgtctt gtctagaggc tccgatcatc tcaaaacttt
     3121 gctccaattt attaccaagg gtggcggtgg catagacatt gtcgtcaatg tttttggtgt
     3181 cattattaca ctactatttc tcaacccaaa atatgcccta ctctgtcttg ccaacgccat
     3241 tatttatctt tcaatcttag cctaccaaac caagaagctc aaaggtcaac gtcatcaaat
     3301 taatcaaata tccgacaagg tcatgggtaa aaactttgat tatttttcca acatgtctct
     3361 tataaaaagc ttgggaatag ggcaaaatat caaccgagtc ctttttgaaa aagaaactaa
     3421 actaaccgag aaatcgattc gctacaacac tttggaattt aataaatgga tatacatcaa
     3481 caccatttct caaattttta accttatcgc catctatctc gttgtcaccg acatcaccaa
     3541 aggttggatc accctaggat cattttttgt ttatattggc tacatcaacc gtctacagga
     3601 tggcttaggt gatattgccg actggatcaa tgatctaact gaaaaatatc ttggattatt
     3661 tcgtgtccac cagcttctaa aatcaggtac tagcaccact gaaaaaggtc acattacctt
     3721 tccccgtcag cttgataaaa tcagtctttc tcaaatgagc tttgcttaca acgccactaa
     3781 tcccatcctc gataatatcg atcttgaatt atataaaggc aacaaatatg gcctagtcgg
     3841 agtatctggt tcgggcaaat caactctggc aaagctacta ctcaaactct atcctcccac
     3901 caaagggaat atctactaca atcaacacaa aatttgccat attaacactc aatctcttca
     3961 cgataacatc ggagtagctc cacaagacaa cgaagtattt aacctatcat tcaaagaaaa
     4021 tatcgtcatt gcctcaaaca ccagccgatt taataaagag ctctacgacc tcgccattaa
     4081 aacttctgaa tgccaaccga ttctcgacaa aattcacaac aatcacaaca ccatgcttgg
     4141 agaaaaaggt atcaagcttt caggaggaga gaaacaaagg ttaggtatcg ctcgagcaat
     4201 ttacaaaaac acccccatca ttatctttga cgaaagcact tctgctcttg attcaaaaac
     4261 tgaagccaag attcttcaaa acctagaaaa atattttgaa agtaaaacca tcatctggat
     4321 tgcccaccgt ctctctaccc ttcgttttac tgatcagata attgtttttg acaatggcaa
     4381 aatagttgaa caaggcagtt tccgccacct tatcgactcc aaaggacact tccacgacct
     4441 ctggcaaatt cagaaaaaaa ccaatttaaa atagattcgt gttaaaatca aaatcttatg
     4501 tcctcctctc attgcggtat tgattttggt accaccaatt cttctcttgc aatatccagc
     4561 tcaaacatcc caaaagttat ccctatagac agacaaaaca caaatccatc tattctccgt
     4621 tccctcattt acatcaaccc cagtcaacaa accagtattg gttttgatgc catcactcac
     4681 tatttaaacg atttaaaaga acttcctagt aagcccttac gcgtcgtcaa cactggccgc
     4741 atgatcaaag tcatgacccc taccggcagt ggagttatga aagccgactg ggtccctgaa
     4801 attatcgaag tcgatgagag tggtcgtggc cgtttacttc aatcactcaa atcagtcctc
     4861 accagttctg tttttgctgg taccaacatc tttggccaat tttacactct cgaagaatta
     4921 ttaaccattc tattaaaaga aataaaaact cgtgccgaaa aagaaattga tcacgaatta
     4981 acttcagccg ttatcggccg accagtcaag tacgttggca cctcagaaaa caaaattgcc
     5041 ctcgatcgta tgcgtactat tgccaccaat tctggcttta aacatatcga atttgaatac
     5101 gaacctgtcg gggctgcctt acactacagc tctcaaaacc cgactcccca gaatatcctc
     5161 gtctttgatt ttggtggtgg tactctcgat atctgtatta tgaaactacc cgagcaaaaa
     5221 atcattgccg tctccggtcg cggtattggc ggagatttgc tcgacagtca aattgttaag
     5281 tcctactttc tccaccactt tggatcccaa agcataatta acgaccacct ttccttcccc
     5341 aaacatttct ttacagcttt cgacagttgg tatcaaacta cacttcaaaa aaatattaag
     5401 aacatcgatt cacttcgaaa aatggccatt gagtcagaca atcctgcctc aatagaaaac
     5461 ctaattaatc taattatcca cgactatggt tttgaatttt ttcaatctgt cgaccaggca
     5521 aaaataaatc tctccaccct agatactgtc
//
LOCUS       DDWP01000047            2807 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_20547, whole genome shotgun sequence.
ACCESSION   DDWP01000047 DDWP01000000
VERSION     DDWP01000047.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_20547"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          931 a          489 c          567 g          820 t
ORIGIN      
        1 atggcaggaa caaagtaggc agaaattcta tcggcaaaat cttgaatcgg agctttggaa
       61 ctttgagcat cttcaactaa tttgatgatt tgagataaag tggtgtcggc aactaatttg
      121 gtaactttca tctcgaaact gccagataaa ttttgagtac cggcatagac tttggcccca
      181 atagttttgt caaccggaag gctctcccca gtcaaaagtg attcatcaat agagctgtcg
      241 ccggaaataa taatgccgtc gaggggaatc tgctcacctg acttgataac caaaatttgg
      301 ccaagtttga cttggtcaat atcggcatat ttgccacgta gagccactaa tttactaagg
      361 ctgtctgaag ttttaccttt agctaaactt tcaagatatt tgcccaaaat tacaaaagta
      421 atcaagaagg cggcgacctc aaaataaaga tgaggaatca tcatgccgtt aagccctaag
      481 aaagttctgg tttcggaata atgaacaaag aaagaataaa gactaaaaaa ataagcagag
      541 ccggtaccca gggagacaag gctgtccatg gtaaaagtac gaactttaat agcggacaag
      601 gcccctctaa aaaagttgtt gccgatgaaa aacaaaataa tggtggaaga gaaaagagaa
      661 attatcccag cataaggcat tatttgagcc ctaaatgaga atttggggaa aaagtcaaaa
      721 aacataaaaa cagctaaggg aagggataga agagtggaaa agattagttt tttgcgccaa
      781 taatttattt ctagagtacg tttttgagat tcggtaagag agttctcatt ttcgggcatg
      841 gcgccatatc ctatctcttt gacagtagaa atgaatttat cgactgtcga tgtcaacgga
      901 tcatatttta cagtggcctt gccggtagaa tagttgacat tggcttcaag aacacccttt
      961 tgtgattcga gggatttttt aattaagagt gaacatgagg cacaatgcat gccggaaata
     1021 tcaagctttt gaactttagt gttgatcatt actacaagtg tagtagatgt aaatcaagat
     1081 agcaactact tagagtttat ttagggatga tctcaacttg tcaacataag caagtctgtc
     1141 tttgggctca gacaagatac tctttttaga ctgatttaag tcgaaagagt caccttcata
     1201 ccgaggaaat acgtggtggt gaaagtgaaa agcatgttgg ccaccggccg gctcgttgtt
     1261 ttgcctggtc gtaataccgt cacactggta agaaactttc atagcaattg aaactttttt
     1321 gatcacttca aacattctat ggccaacttc aagtggtagg tcgtatatat tttcaaagtg
     1381 ttgattgggg acaacaatag catgacccat cacagtgtca agccagaatg agttgataaa
     1441 aacgctaaca aggtcatctt taaaaactaa atcggcttgc tttaacaaag tatcttcgct
     1501 ttcaacacca ctgaccccta agcaaattgg gcatttgtaa ttatctgggg aatgattgta
     1561 catatgatgt tgaaactggt aactagacga tgatactaaa agattgtagt ttctggacaa
     1621 agtcctcaaa tgtcgtatag acaatagcat tgatacccag agacttagcg gaaataacat
     1681 ttgcctcttt atcatcagcg tagaatattt cttctggcag tacctcagat ctatcgatga
     1741 gagtttgaaa tatttttgga tctggtttta ctgatccaac ttcataggaa aaaacttgga
     1801 catcaaaatc tgagagaaag tcgaacttct tacctacggc gttgatgcga gtagggaaat
     1861 tgttagtgca aatacaagtt tttatacctt gacttcggac ttttttgaca tagtcgacaa
     1921 catcttgatt gacctcataa gcctgataca ttaactcaaa aatttgttga ttagaaagat
     1981 ctatcccaat ttcctgacga gcaatgtccc agaattgatc ttcggttaat tctcctcttt
     2041 taaaggacat catcgactct gatttggaga gaaaatctaa tttagtaaga tctgaaacag
     2101 ggagagaatt cttgaatcta gaaaagctat tttcagtaaa atagacacca tcaaggtcga
     2161 aggttatggc tttgaccatg agttacttct gacaaattaa acgaataagt ttgttgtcgc
     2221 catctttgat ggtcttacca ttgttatcga gtaataagat cttgaactcc tttggaagta
     2281 aaagttctaa tgaatcaacg ctaaaaaagc gtttgtttat gccgacaggg gttagataaa
     2341 aatcatcttc aattcttgtt gataaagaaa cctctggatc agtcaaagaa ttgaatagag
     2401 tacaaaataa accgttaggc cttagggttt tgtagatttc ggaaaataat tgtaccgtcc
     2461 ttttgatagg aaaataatgt aaacctaaat ggctataaac aatgtcgaac gactcatctg
     2521 gaaaaggcag tggttgattc aaatcgacta attgagaggg aggtataaga tcagtagcga
     2581 taacatcata acctagacta gaaaaatatc tggaatcttg gccaacaccg gatgctaatt
     2641 caagcaattt gccttgttta ggaaaatact cgacaacttc tttggcaaat atatttggat
     2701 gttttatcca ttcttgggtg gaatatgagg caagtttttt agtccaataa tctttcagat
     2761 ccatactgga ttatacatct actccaccac ttcaatactt ccagaaa
//
LOCUS       DDWP01000048            4967 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_29220, whole genome shotgun sequence.
ACCESSION   DDWP01000048 DDWP01000000
VERSION     DDWP01000048.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_29220"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1402 a         1033 c          943 g         1589 t
ORIGIN      
        1 aagacagctg tcgccgagag aggcgccctt attagctctg tgtctgctac cggaactatt
       61 acttctggca acactactta tatcactacc ggtgctactg gtactgtcaa aaaagtttat
      121 gtcaaaaatg gagatgtcgt taaaaaaggt cagaaattag ctgaaatgac tctggatgac
      181 gaaagcgccg agactcaaac taccgcctgg gccaactacc tagtcgcttc tgaaaatgtt
      241 aaatcagctc aagccgctcg cttaagtgct gatttacaaa tgtggaaaga tcgccaagct
      301 gttcttgacg ctcagacaga atacgacaac atgattgccg gtgcttggaa ccccaaaacc
      361 aaagccgaat acacttacaa cgagaaagct gtggttacca aaactctaga gctggctaaa
      421 ttgaccttca ctgccgacga atctaaattt aacaactcag gtgttgatgt ttccttagcc
      481 aagacaaaaa tggctgctgc cctcagaagt taccagcaag tcagtagtac tatttatgct
      541 cccgcttccg gaatcctcag caactctatt cttgctgaag gtgtggttat ttccgacagc
      601 tcttcaactt caattactgt cagttccggg actgactcta ctaccaactc tcagtcagtt
      661 actgctcaaa aaatcggtgc aattaaaaat cctcaaggtc aatatcaagc cactcttagt
      721 ctgactcaag tcgatgtcac caagataata tctggtcaga aagttactat gactatggat
      781 gctttccccg acatcacttt taccggtaca gttctggcta ttaacactag cggtagcgtc
      841 aactccaatg ttacttctta ttctgtcact gttcttcttg attcgactga tcttgacata
      901 tataccaata tgggagtctc tgccaccatt atcactgcta taaaaaatga cgttgtctca
      961 attccttcta ccgcaattaa aaacaatact gtccaaatct taaaagatgg tcaacctatc
     1021 tctgtccccg tagaagttgg tgtgaaaaat gatactaatg ttgaaatagt ttccggccta
     1081 agtgaaggtg atgtggttat tatcagtact gtctctggca ccaagaccac caatagttcg
     1141 tcagggactt ctgtctttgg cagtactcgt ggtagtggca tgatgattcc tcgctaaact
     1201 atgcaatcaa ttattaaagc cagtcatatt agcaagatct ataagacgga gtctttagaa
     1261 actatcgctt taaatgatgt ttcttttgaa attaaaaaag gtgagtttgt agccattatg
     1321 ggcccatctg gttcgggtaa atcaacctta atgcatatac ttggcgctat cgacaagcca
     1381 acctccggca cctatttact tgacggtgag aacgttggct ctctggatga tgatcaactt
     1441 tctgatattc gtaatcgtaa aataggtttt gttttccaag cttttaattt gttaccacgt
     1501 actactgctt taaaaaatgt catgttgccc atggcctatg ctggtatacc taaggaagaa
     1561 aggcttgaga ccgctactaa attcctcaaa ctagttggcc ttgaagacag aatgtttcat
     1621 accagtaatc agttgtctgg aggccaacaa caacgtgtgg ctattgctcg gtcattggtt
     1681 atgaatcctg ccttaattct tgccgacgaa cccactggta atattgcttc cgaccaggcc
     1741 gaagaagtta tgggtatgtt taaaaaacta aatcaagatg gtcacaccat cgtcatgatt
     1801 actcatgagc cagatatcgc tcatcacgcc aaaaggatta tttatgttcg tgacggtaaa
     1861 ttattcaaaa gtctatgagc tatctagaat tatttgttga aagctttgct actttggccc
     1921 taaataaggt tagaacaggg ctagctattt taggtatagt tattggtata ggttctgtca
     1981 ttgctcttgt ttctttgggg caggccactc aacagtcaat tgagaatcag attcaatctc
     2041 ttggagctaa tctcttgacc attaaccctg gtgcacaaag aagtggtaat gttcgcacag
     2101 cttcgtctag taatacttta actaacgaag acgccgctgc cattgctagc tcctcagaga
     2161 tcactactat aaaaagtgtc tcgcctgaat actcatctcg ttctcaagtc gttactggta
     2221 gcagcaacac caatacctct atcattggtg ttgttcctgc ttatctagat atccacaaag
     2281 ttaccattga ttcgggtagt tttatttccc agtcagatgt tgatagtctg tctcgagttg
     2341 ctgtcgtcgg tccacaaata gttgccgact tattttctgg atccaatcca attggtcaag
     2401 tcataaaaat tggtagtcaa aactttacta ttattggagt tactgtcagt aaaggtggta
     2461 gtggttttaa caatcaagat gacgttattt acgtcccgtt gtccactgcc caaaaaatat
     2521 tatttggtgc cagccattta acttcaattg ccatggaggc caatagctct gatattatga
     2581 ctgccgccca agatgaagtc ggttattttt tactatcccg tcataaaatt actgatccat
     2641 ctaaagctga tttttccatc ttttctcagc aagatatatt aaacacggct tcttcaacca
     2701 caggtacttt tactaccctt ctttctggtg tagcggctat ctcactctta gtcggaggta
     2761 tcggtattat gaatatcatg ttagttacag ttactgagag aactcgtgaa attggtctca
     2821 gaaaagcttt gggtgccaaa aaaagaatta tcaccattca gtttctatta gagtcaataa
     2881 tcctaacttt tactggaggg gttattggtg tgctccttgg cattcttgcc tcttatatta
     2941 tttcggttgt ttccggttct ttgtttgtga tttctttttc ctcaattttt ctagcttttg
     3001 ctgtctccac tattatcggc attattttcg gctggtatcc ggctcgtcgt gcatctaacc
     3061 ttcaaccaat tgaagccctt cgttacgaat aaaatttatt aatttttaaa atatgcaaaa
     3121 aaatatcgtt cccatcatca ttatcgctat attattttcc ggaattggtt ttttctctgg
     3181 aacaacttat caaaaaggca aaatgccatt aagaggtaac caattgcaga ataataatat
     3241 ccaaaaacgc ccaggtaacg gtcaaataat gggtgagatc accagtgtca acgacggttc
     3301 cgtcaccgtt aaaactactg atggtagttc aaaacttatt ttacttagtg acaaaactaa
     3361 tattaaccaa gccgtgcctg ctactactac tgatttggtt gtcggtcaaa aagttgttat
     3421 ttttggtaat accaatactg acaacagtat ctcagctcaa agtattcaat taaatactag
     3481 ccttctacaa aagtcagagc gtaaccctta gcatcacctc ggccagtacg accgattctg
     3541 tgggtatagt catcaaagtt gtttggttgg tcgtaattga ttacgtggga aatatcagat
     3601 atatccaatc ctctggctgc cacatctgtt gcaatcagaa tattaaatat attttcacga
     3661 tacgctctaa ttacttgagt tcttttattt tggggtttac caccgtgcaa gctttgagcc
     3721 ttgaaacctt tctgtgataa agaagttgaa agttgttcca caattctttg agttgctcca
     3781 aacaccaata cttttttgaa ttctggcttg gacatcattt catgcaactt ctcttctttg
     3841 agatggcgtg gcactcgcac cacatcttgc tcaacatgga ttgatggatt catgttcttc
     3901 aaagagatcg acactggatc tcttaaaaaa gaaccaatta atccttcgat ttttcggtca
     3961 attgttgctg agaaaaatag tgtctgacga tccttaggag tctgatcaac aattgatttg
     4021 atatcggcaa taaagcccat atctaacatt ctgtcgactt catctagtac taatgtggta
     4081 attttcgaga aattaattac tcttctttca cccaaatctt ttattcttcc tggtgtacca
     4141 acaataaagt gtggtcctcg tctaatatcc attatttgtt ctcgtaaaaa agatcctcca
     4201 atagccagag ctaggtaaac cctaagcccg ggggtaaagg aacggatatc ttgtttgatt
     4261 tgcatggcta gctctctggt tggagccata atcaatatct gttgattagg attttccagt
     4321 atggagttaa ttaaaggaat ggcaaaggcc gctgtcttgc ctgtacctgt atcggcaata
     4381 cccaaaaggt cacgaccgtc cataattgat tcgattgcct ggtcttggat ttgagtcggt
     4441 ttggtaaaat ttttggcttt gatattttct aaaagtgatg ggtttaaacc aaaatcttca
     4501 aagctgtgaa tggttgtttg ttcttcaata gtcactattt ccgtcgcctt tttgataaat
     4561 aaagacgggt tcatttttgt ctgagagaat cgtggtcgtc ttggtcctcc aaaactaggt
     4621 ctgctaggac gtcccccaaa agcgggacgt gattgtccgc gaccggaaaa atttctaaaa
     4681 ccaccttgag aattactgtt gcttctgtac atataaaatt aaattttagg caaattccac
     4741 aattgtggat tatgtgccct taataaaact tatgtgtaca gtcttctgta ccaatagggc
     4801 agatatcgat ttctagagcc tttaaactaa aatcagatgg aagtgtacca tatatgtaaa
     4861 tatatgcaac tttttctgaa ttatttgtta ttataagtaa tgtctcaacc ggtttatact
     4921 gtcaagattg ctggtccagc cggcgctggt atcaagagta gtggctt
//
LOCUS       DDWP01000049           12794 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_15178, whole genome shotgun sequence.
ACCESSION   DDWP01000049 DDWP01000000
VERSION     DDWP01000049.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_15178"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3520 a         2656 c         2233 g         4385 t
ORIGIN      
        1 gcttctatca aatcaatctt ttatcgtccc gagaggacta tcactgctgt tgacgatatc
       61 agttttgata tcaaaaaagg tgaactggta ggttttattg gtcctaatgg agcaggcaag
      121 acgactacct taaagtgtct ttctgggctg ctatttccta catcaggtac agtcaatatc
      181 ctgggtttcc agccttctga ccgtaagtcc gagtatcttc atcagatagg ttttgttatg
      241 ggacagaaga atcaactttg gtgggacatc cctcctcagg aaaccttttt acttaataaa
      301 gctatttaca acatctctga cgccgactac aaaaaacgtt taggtttttt tgttgacacc
      361 ttagacatta aagacattat ctccgttcaa actaaaaaac tctctcttgg tcagcgtatg
      421 aaatgtgagt ttgtcgccgc tttacttcac aaccctaagg ttttattttt agatgaacca
      481 actattgggt tagatgtggt tgctaatttg aaaatccgtg aattcatcaa gagtgtaaat
      541 caggaattta gcactaccat tattcttact tcccacaata tggacgatgt cgagcagctt
      601 tgttcccgag tcattttaat tgataaaggt aaattgcagt ttgatggtaa gttaacccaa
      661 cttaccaaga aatataccga gtcaaaaaat atcagtttta ctcttgaaaa aaacgtcaaa
      721 tcaaaagacc tttctccact tggtcgagta gtcactaccg gaccacttaa ctactcggtc
      781 aaagttccca aggataaata tctcaaggtc gcttcaacta ttattagcac cttgcctata
      841 tctgacctta acattgaaga tactcctatc gaagatatcg ttcgccaaat ttatactcat
      901 aacaatggtt aaatatgtaa gaatagccat caatactctc caagaatatt ttgtctatcg
      961 aatcaacttc ttattctggc gagttcaggt ctttgtcact ttcgtcatat tctttagtct
     1021 ctggtcttca gtctctgctg gtcagagtag tctcggggct tattcaattc cccagcttta
     1081 tagttatttt gttgttggct atgttatccg ggcttttgtt tttactacca gaaccgccga
     1141 tatcggtggt gatattcaaa atggcaacct atcttcacta attattaaac caattgggac
     1201 gattaaatat tattttagcc gtgacttggt cgataaactt tttaatttgt tcttcatgtt
     1261 ttgggaattt ttactaattt tatttctctt caaacctgtc ttagtaattc cctctgtttt
     1321 gaattttgtt tttttccttt tgtttttact agtttcgacc atcaccttct ttttttacag
     1381 tttgataatt tcctttttaa ctttttggtc ggataatgcc tggccgtcac gttttctctt
     1441 tggagttgtt tttgtcacac tttttagtgg gcaatacgtt cccctagatt ttcttcctcc
     1501 atttttggct cggattattg actatacccc ttatccgtat atgtattatt atccggttag
     1561 gatctggctt gggcaaacct cacctgagat ggctcttacc catctaatgg gtggtctagt
     1621 tgcctgtctt ggttgcttcg tcgttgctca atttctctgg ttaaaaggta aacacaaata
     1681 ccaaagttat ggcaattaaa actttttttc gagtctggac tattttggcc aagaacaatt
     1741 tacagaatca attactttcc cctagtgcct ccttactttt tgtggttggt aagatattca
     1801 atttcatctt cgccattatt actatctatg ctatttttaa ccagacttcc tctattcgtg
     1861 gttacgactt acctcaagca gttatcgttg ttctagtttt tagtttcatg gatacattaa
     1921 ctcaattttt atttcgttcc ctttacactt ttcgaccagc actgataaaa ggtgactttg
     1981 atatggactt actcaaacct ttaccatctt tctttcgtcc tattttttcc ggcccagatt
     2041 ttctggatgt cccgatgatt atcatccaac tattttctat tttttatttt attgctcact
     2101 tttctatcac cattactctg ccaaacctac ttgtcttctc tttagttttt gtaaatggtg
     2161 tagttatcgc cttttcggta catttaatta ttgccgcctt cgctgttatg actagcgaga
     2221 tagaaaactt agtcatgatc tatcgagctt taggtcgcgc cgccattgtc cctaccgata
     2281 tctatggagc tactttccgg tttattttaa gttatgtcgt ccctattact gtgattatga
     2341 ctattcctgc taaagcctta ctcggcattc ttagtattga gagaattatt tactcaacga
     2401 ttctgacttt agtattttta attttttccc tgaacttctg gtctttttct ctaagaagat
     2461 acactagtgc tagtagctaa cttgccccat tgactatatg taactggtac gtataaactt
     2521 atcttcatga ctgcagaaaa taaacaagaa aaaactcctc cggcaactac ccgtcctcca
     2581 gcttaccaag aaccaaaaac tactccattg gtcattccaa ggacggtggg tcatcaagct
     2641 gcccttactc agctgaagga attgcaggaa aggtacacta ctgaaacagg ttttgtattc
     2701 aaaagacgct aaatcggtta caatacctca tgtcaaatat tagtatcggt attgttggtt
     2761 tgcccaacgt gggcaaatcg actctcttta atgctctttt gggtaaacgt gttgctgatg
     2821 ctagcaatta ccccttttgt accatcgatc ccaacgttgg tgttgttgaa gtccccgata
     2881 gtaaattacc tattttagga gatattgtta aaactcaaaa aatagtccct gccattgttg
     2941 aatttgttga tatcgctggt ttagtcaaag gtgctagtac tggtgaaggt ttgggcaata
     3001 aatttttgac caacattcgc gactgcgatg ctattatgca tgtcgttcgt aatttctctg
     3061 atccaaatat tatcaaagaa ggtagtgtcg accctcaggg tgattttgaa gtcatttgta
     3121 ccgagctgat tatcaaagat cttgattctg ttgataaata tttatctctc aataaaaata
     3181 accccaaaga gaataaatcc aagaagtttg ctttaggtga gaaattaaag atctctttag
     3241 aacaaggcaa actagccatc aatctagatt tagatgatga agaaaaagtt cttctaaaag
     3301 aatttttctt attaactgcc aaaccatatt ttattgtcgc caatgttgat gaagatacct
     3361 attccagcat ttccgacttc aaaaccagtc tggagaacgt gattccggtt tgtgccaaga
     3421 ctgaattcga tcttactgag ctagacaaaa ccgatcagca aaattattta aatgaattag
     3481 gggtcaagac ttcaggtctt gaaagagtta ttcaaaaagc ttatgagact cttggacttc
     3541 aatcttatta cactgccgga gttaaagaag tcagggcttg gactatccgt caaggctaca
     3601 ctgctcctca agctgctgga gttattcata ccgattttga aaggggattt atcatggcag
     3661 agatagttaa ctatgccgat tttgtagaaa atagtggttg ggatggcgcc aagacaaagg
     3721 gtctgcttcg tcatgaaggt aaaacctata ttatgcatcc cgacgacatc gtcgagttcc
     3781 gctttaacgt ttaggtgttc gtttccccaa gttttggtta acaacaattt tgacagatga
     3841 tccggtgtct gctttgattc tttgttgaat attaaataca atatctgttc gtgcttctac
     3901 caccacttct ttatcccatg gatataccgc agttctggaa gatattgttt tgtctaagag
     3961 atcattaagt atcttgcgag cctttggttc tttagcaagt aactcaggac tatcaatgta
     4021 atcaaattct tgctggttga ccctaagtaa tagtacttca ccgaactttt ttccacgaat
     4081 atgtctgatt tccataatag tatattatat acacatctaa tatgttcgtc aactaaggca
     4141 caaatttaat atataatcag ttaatatctt gtttgcaata caactttgaa aaaaatccta
     4201 attatctctg gtcccacggc taccggtaaa acttctctgg ccataaaact tgctaaaaaa
     4261 cttaacggtg aactaatttc cgctgattct cgacaaattt atcgcggtat ggacattggc
     4321 accggtaaag atcatcccaa agatgtcaaa atcaatttaa ttgacattat cgacccagat
     4381 cagtctttct cagttgagca atatcgtcaa ctagccgtag agaaaatcaa ccagattcat
     4441 gcaaatggta agttaccgat tattgctggt ggtactggtc aatatattga ttctattacc
     4501 aatcctcaac ctactttttc gataaaaccc aacaaagtat taagattttt tcttaaccac
     4561 tttcctctta aacttttaca gctaacttta agacttttag actttaagac ttttagacta
     4621 ctcaacaatt ctgatcttca caacccccac cgtttaatta gaaaaataga aataaaacta
     4681 agttttaagt ctcccttgtc aaaggaagat ttagagggat tgaatacgtt gcacatatcg
     4741 ttaaccgctc ccaatgatta tctctattct cgtatcgacc agcgtgtcga atcccgtctt
     4801 aaattgggtc ttattggcga aataaaaaat ttattaaaaa aatataaagg gtctgatccg
     4861 ggtttaaatt gcctcgccta taaagagttt gtaggggcgg atcttgtgtc cgtccctcaa
     4921 aaattaatcg atatttggcg ttttcatgag cattcctatg ctcgtcgtca aaaaacctgg
     4981 ttcaaaaaaa tccccaatat catttttttt gacatcacca cccccggttt cgaagctaaa
     5041 ataattaaag aagtcggtaa atggtacaat aattcatgac tattcaaaaa gttgagttat
     5101 cttataaaac agttgttttt accgttgcct ttatcctcgg attagccctt ctatggtctg
     5161 ttcgagacat tctcattctc ctcttcattt gttttgtctt tatggaagct atcaatccaa
     5221 cgattactcg catggagaag gttcatatac cccgtcctct ggcaataatt attgtctata
     5281 tcttggttat tggtatattt ggagtagcct tggctggcat ggtaccaatc ctagttaacg
     5341 agaccagtga acttatccga gttttacctg gtacattaga aaatttccag ttttttggtt
     5401 ttactgcggt agacttgtct tcgcagttta agattttaga atcaattcct ggtgatattg
     5461 ccaaaacaat tttttcgtta ttttccaatc tattttcagg ctttgtaatc ttagttatta
     5521 ctttctacct tcttctcgag aggcgccatc taaacaaata tagtcttcaa gtctctggta
     5581 gtaaaggtca agctattatt gtcaagatct ttaatcagct tgaggatcgt cttggtagtt
     5641 gggttaatgg ccaaatcgtt cttatggtcg tagttggtct actttcttat attggttatc
     5701 tggttattgg tttgccttat gctctgccac tcgctcttat tgctggtttg ctagaaatag
     5761 ttcccaatat cggcccaatt atctccaccg ctttagctgg actaattggc tttactgtct
     5821 ctccgttact tgccgttctg gctattgttt ggggcattgc catccaacag gcagaaaata
     5881 atttcattgt acccaaaatt atgaaagaaa ctgtcggact caacccggtc gtaaccattc
     5941 tgacaattgc tactggcgct aaactgggtg gagttggtgg tgcattacta gctgtgccga
     6001 tttttctgac tatcgagact attgtccgag ttctcatcaa caaaaaataa cctcaaaaga
     6061 aatagttaag ccggattctg tttatgggcc atcaatctat tctctctacc tgaagctctc
     6121 aacagttaga tattaacgct tcatcttgag atttccgcgg gaaggttgct cgtttcacat
     6181 ttatcgtctc tgtagcagcg ccccgacctt acggccgtct ctatattttt aagttcccta
     6241 ctgtttgcgg tccggacttt cctcgggatg gtgtccgtga ggacatgata tcccgaaagc
     6301 ccttctattt cttttgaagt ctctatattc taacattatc gaaaaaaatc ccctctccat
     6361 agttggaaag gggatttcta tttcaagaaa attatgcaag taaggctgca tctgctgcag
     6421 acaatatttt gaacattttt gttcctgact ctggatcgac ggctgatgcg gctggtctac
     6481 cgtttttcaa catgaccttg gtcacctgag acacgtcaac ttcaactgat tttctcagtt
     6541 taactgaata aaattttatt actgccatta tttcttcacc tcctaaggta tataaattat
     6601 taattttggt tttgcaaata aaacatgact cctatcacta tcacacaaac acagcttagc
     6661 acaaaacttt tccacaaatc tttttttata taattacttt ctttcattta ggttcaaaat
     6721 cagtatagca taaagtattt tgttacaata acttgatatg aataatttat tattacttct
     6781 cgctttactc aacacttttt ggctagctta ccttactttt attgtataca agaaaataac
     6841 cgctattagt catacaaagt ctgattccac agtccagcat tcttttaaag tcggtttaag
     6901 tcgatttaat ccttttcaaa atatgggtgg tgaccaaagt tttgttttgt gtctgttgga
     6961 taacactaat tcaggtgcta ttattacttc attgcataac cgcgattcta ctcgtgtcta
     7021 tgccaagcag atcaaaaatg gtcaaggtga aagccagtca ctatccgacg aggaagttaa
     7081 gcttttggcc aaaacagtta gtaattagac cacaaacata tcaatgaact ctaaattgac
     7141 tcaaataagc acttacgctg gtatatttct attcttgctc ggaatttcat attatggatt
     7201 tgggcaattt ttccccacta ctagtcaagg caccaatcct aatggcactc ctgcagtcac
     7261 gctcgctcct caaaaaacag gctctattaa tgtttcaggt ccaaagatag aagtttgtcc
     7321 aatcaatggt gacttattta cttcagaaga gaaagatata tggtcaactc gccgtcctat
     7381 ggccgttatg attgagaacc atcttgactc tcgtccccaa tctggccttc aaaatgccga
     7441 cattgtttat gaggctgttg ccgaaggtgg aattacccgg ttcatgggaa ttttttattg
     7501 taacgctgct aagggttctc aatcaaaata tgatgttggt ccagttcgtt ccgcccgcac
     7561 ttattttctt gacttagctt ctgagtacgc cgactatccg ctttacgctc acgttggtgg
     7621 tgccaactgt agtgccgcta ctccgggcgg tccctgtacc actgccaaga aagcccaagc
     7681 cattgaacaa atatctcagt atggctggaa taatgctggt acctggagtg acttgtctca
     7741 gttctccctg tcttacaaag cttgccgtcg tgagcctgaa agaaccggtg aggttagagc
     7801 taccgaacac tctgtttatt gctctactac tgaaatgtat aatgtcgctg ccaaccgtgg
     7861 tcttaccaac cttacaactg tcaataaaac tagttgggac aaaaactacc gaccttggag
     7921 ctttaagcag aaagactctc ctgtatcgac cgacgttgtc gccaacatat cttttgattt
     7981 ttgggctgga tacaaagact attctgttag ttggaaatac gattcatcta ccaatcgcta
     8041 catcagaagt aatgatggtc aggtgcaaat tgacttcaac actcaagaac ctttgtacac
     8101 caagaaccta gttgttcaat ataccaagga aacacgtgcc gttgacgtgc atggtcacct
     8161 cctctacgac ttaattggct caggtactgg aatcctttac cagaatggtg gtcgccagga
     8221 gataacctgg tctaaggcca atagaatttc tcgaaccata tataaagaca agtccggtgc
     8281 tgaggttaac tttgttccgg ggatggtctg ggtggagata ttaccaattg gtaacaaaat
     8341 aaattatgaa ggtcagtcat agtttcaaaa aacttcttac ttctcagacc aggcttaagt
     8401 taattaatat atttttttat tctccgatcg aaatatatta tgtccgtcaa cttgttagat
     8461 tgactggtga agagataaat tctgttagac gagaacttga taacttattg tcttcatcta
     8521 ttgttctcaa agaaactaga ggtaatcgtc tttattattg ggccaatccc gatcactact
     8581 tatactacga tttagttgtt ctggccaata aacattctgg tctcggagct agtttccaag
     8641 aaaacagact aaaattgggt aaattacgag ctctttttta cagtcaggac tttacttttc
     8701 attctcaaga caatcaagat agcatcgaca tgttgattat tggcgacgtt tctttaaaga
     8761 taacccaaga tctagtcaaa cctgaagaat ccactcttgg acgtgagata aattatatgg
     8821 tcatggatcg gtcagagctt catcttcgtc tctcaaaaag agatccagtt ttggttgatt
     8881 tctttttaaa gtatcctgtt ctagttatcg gcaacccctc caatttcatg aaataatggc
     8941 aaactttaga aaagtcatca agacaggcaa tagtctagca atcaccatcc cttctaaagt
     9001 tgccgcttct ttctctctta aagaagggga ctttgctcaa ttaaaagtca atcgcaccaa
     9061 gacttctcta acttatgtct tcactggtca ccctcgacaa ttgtcactta ttagtcaatc
     9121 aaaaacaaaa tgaacactcc tcctgtagct aaaaaaactt ggaaagtatt ttggagcata
     9181 attgccatgt ctgtcgtggc actagttatg aatctaccct ctgagttttc tctttttggt
     9241 agaagctttt atcgtcccaa ttttgctatt aatattgccg gtaatactta tagtaaagac
     9301 cttgatctga aatacggtct agaccttgct ggaggttctg ccctggttta taaagttaat
     9361 tcagaaaacg tgtcttccgg cgaccttcct tctgctctag agtctcttaa gggcaatatt
     9421 gaaagaagag ttaatttatt tggtatcagt gaggccaacg tccaactgtc aaccgttggc
     9481 gaacagcatc gtttgactat tgaattaccc ggtgttaccg acataaatca agccattgat
     9541 ttaattggta agactgccca gcttagtttc aaaggtataa tcgacgttgc tccagaagct
     9601 actagtaccg ccactattga tgatgttctt aaggacactg gtctcaatgg ttctcaccta
     9661 atcaggtcaa tgccccaacc aaatccaaat actggtcaaa tcgaagtttc tttagagttt
     9721 gatgctgagg gcgctaaatt gtttgaaaag gcgaccaagg actatcttaa taaaaggatt
     9781 gctattgttt tggatggaga agtcattact gccccaactg ttcaatcagt tattcccgat
     9841 ggtaaagcag ttattaatgg taactttgac acccagtccg ctaagtctct cactgctcag
     9901 cttaatgctg gtgccctacc tctttctata gagctgattc agcagacaac tgttggtgca
     9961 actctgggta aagactcagt cgaaaaaggt ttttatgccg gcatggttgg tctactacta
    10021 gtggctatat ttatgatcgg taattatgga tatcttggtt tcatctcctg cttggcatta
    10081 ataatttatg gcctcattac tctcactctt tatcgtctaa tcccagtcac tttaactttc
    10141 cctggtctgg ttggctttat tctttccatc ggtatggcag tcgatagtaa cattcttata
    10201 ttcgagcgtt tccgtgaaga attacgagac ggcaagccct ggagtatagc tatggagctt
    10261 gcttttggta aagcttggga ctctatcaag gatgctaaca cttccactat tattaccggc
    10321 ttaattctct ttaacccctt taattggagt ttccttaaca ctagtggtat ggtcagaggt
    10381 ttcgccgtta ctctaattct aggtattttt attagtatct tcactggtgt ctttgttacc
    10441 agaaatttat taagagttct cgcccgtaaa aactaactat gaatatcatg aaatttcgtc
    10501 cacttttctt tgtcatttct tgttcactcc tattgatttc ttctttctct atctggaaat
    10561 ggggtttcaa acaatcaatt gacttctcag gtggtaccgt ttgggaattt caaactaccg
    10621 ctgatattca aaagactttt ttagaaaata atgtcgatat tatctcttta aataatcaaa
    10681 ctgttaaatt caaaagtatt aaccaagaag aaaaattaat gcttgagtca agtttcaaga
    10741 aggcggatcc taattttcaa gaattacgtt tcgagactct cggtccaagt cttggtaaag
    10801 agttaatgca aaagaccgtt gctgctatca tcctatcctc gctattcttg cttctgttta
    10861 ttggttccaa gttcaaagac ttttcttttg gtgcttcagc tatcctggct atgttccatg
    10921 acgcttttat tcttattggc gctttctcta ttttgggtca tttttttgga gccgaactcg
    10981 acgctctgtt tgtcaccgcc ctactcacca ctctctctgc ttccacccac gatactattg
    11041 tgactttcga tcgtattcgc gagttaaaga ggaaaaatat taaagttgaa tggctgcctt
    11101 tagccaacca agctgtttct gaagttatcg ttaggtcaat taataactct atgaccatta
    11161 tcttcatgct cttggcccta gtagccctag gaggggagac aacccgttgg tttgccactg
    11221 ccctacttat tggtgttgtt tccggtactt attcttctgt cgctgtcgcc atcccccttg
    11281 ttttgttttg gaaaggtaga aagaagcact aattcttcag cacatatccc ttccctctca
    11341 ttgtctggat gaaagatcca gatattccgt tcgcctccaa gctatctcta attctctgaa
    11401 ttgtttttga aattgcataa agcgaaaagt cgtcatcact tcttgaagtc acttgggcga
    11461 tttcatcaaa agtagtcacc tgattttcat tttcaattaa tttatccagc accaatgact
    11521 cttgtggcga caaatccata atttttttat caaataacat tggttgtccg tctacactct
    11581 taaaaggttt ttcaaaacta ggcaatccca attttttata aaaagcatcg ctttgtttta
    11641 gtatttctgc attttgtttt gttctagttg tttttattgt tggttcgtaa aattttatat
    11701 catgatgttt actaatcacc ttacctatgg ccgactcagt tattagccaa tcgaccaaca
    11761 tttccgcttc ctgccatgag ccatctagtt ttttgtagat actatctcta gttaaggccg
    11821 acaatatcga ttccactagt ccatatataa agtcttgatc atttctcaag tagactatca
    11881 agccacccgg taacttttct ggtacattaa atgaactttt ggtcccaaat atggttggat
    11941 gtatagtaat acttttgacc gatccgctta gtcctggaat tacttttcct acagtctcaa
    12001 aaaaatcttc ttctatttcc ttccagatat tgttattttt atttatctcc ggttttggac
    12061 aacttgtctt ttcaagcatt acgactaatt catcaattat tacttggtcg atattagagt
    12121 tcattctata tacatcaact ttagacactt tttcccagaa tttattaatt tttttataat
    12181 caagatccgg caaataaaca gcattactat cttggcaagg caacacatta aatccattca
    12241 atcggtagaa tcctaccacc atctgtctag cagtatgaac tattcgtcta gcttcagttt
    12301 ctaggctgta ttcccattta atctccacca cctattatac tataggacat atatcgttgt
    12361 caatttcagt caagatgtat tttaaaacag agttattatc ctcacatgcc agatagaata
    12421 aaagaactga gagcagagtt aaagcaagca aaaaaagaat tgaaagatgc tcctgttgta
    12481 tacacttgtc gaatagatgc tgatcctagt gaggaatgtg tgcgtggtca gcgggtaaag
    12541 gatatacaag aagaattgac gaaattaatc gtgggaagaa tcacttccta gtttctttat
    12601 cttctccacc aacacctctc tcacattcaa gcttggatct tcttcaactt cagcaaacaa
    12661 agcatcgagt acatcaccga cttttctact aggtttaatt tttagtatct ccatcacatc
    12721 ctgaccgtta acttttaggt cttttactgt aaacggttgt ttttgcacct ctaccaatct
    12781 ctttttaaat aact
//
LOCUS       DDWP01000050           12690 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_23640, whole genome shotgun sequence.
ACCESSION   DDWP01000050 DDWP01000000
VERSION     DDWP01000050.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_23640"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3815 a         3341 c         1766 g         3768 t
ORIGIN      
        1 accaaagact cttaccctta caaagattct ctaaacattt tcgaccaaca cataacatat
       61 cttaccgaca ggcttggatc actatctcta gatcaagtta gagatattcg caataattac
      121 ccttgttatc caccaaaaaa atttggttgc aatatattct ccctccaatc agacgccaaa
      181 ttctatggct gttgcgaatc aactatttcc ataggagatc tgtcacaacc aatcaaaaaa
      241 gtaattgaca ggtatactac agttactaat aagcacccac tttgttatga cccagctttc
      301 cactgccaac ttccttaaaa aaactcgtga atacttttgg ggactcggac tagacgaagt
      361 tgaaatttcc tacctcaact catctctccc cctagaacca aatctctatt cttttaaaac
      421 cggagactat tacctcccca cctcccccga gtttgctctc aaaaaatatc tagctgaata
      481 caaaaaagat tgtttctcca tcgcccactg ttttcgccac cttgaaagtt ccggccctca
      541 acacaagccc gaatttctta tgctcgaata ttatctcgtc aataaaaatt tatctgacct
      601 ccaagaatca ctaaaaaaat acttatcact ttttttaagt ttgaagtttg aagttttatg
      661 tttgccggca aatctgccgg ctaacgaacc cgacttcaat caatattttc taaatacaat
      721 cgaaccaaat ttacccaaag acaaagctgt ctttatcacc ggctaccctg ctttcttatc
      781 tcccttggcc gtcgtaggaa cagacctgcg tgtctgttcc cctccacaac caaccgcaaa
      841 gcgttttgag ctatatataa atggaataga aatagccaac ggttgcgaag aaaatcgcga
      901 tgctgaacaa attaagatgg catttgaaac cgaaaaaaaa taccgtcaag ccaacgatct
      961 tcctatccac cctatctctc ttgaattcat agacaattgt gcctccttac ctccctgttc
     1021 cggcctcggc ctcggcctcg aacgtctcat gatgataata aaccagaaaa acagtctata
     1081 atctcattaa catgagcttt gatcaaagac cgccttcgat agatccaagt agattcgcca
     1141 ccccactaga actacaaaca ataagtttac ttgacaaaaa cctacagaag atagccgaaa
     1201 ttactcacga caaagagacc caattccagc tcgccagact tgatccagtt accggcttac
     1261 ctaacaggtt agcctgggac gaaaacatgg aaagcaaaaa aagctctcgc cacccactag
     1321 cagtcttaat catagattta gataagttca aacaaattaa cgatactcat ggtcatcctg
     1381 taggagacaa gtcgcttcat gatttcgccc aaatcctcca caggagtgtc cgttcaccag
     1441 acgatctcat tgcccgatat ggtggtgacg agtttacggg acttatcgat attccgccag
     1501 gtatagatcc tcaaacagtc ctcgataaga ttaaaaaaag actctataag ctacttaaag
     1561 catacaacca aaacaccgaa ataaaaatag gcttctcaat aggtgaatct atttccaccc
     1621 aaccaccaca tgacccaaga catcttctct gtgaagcaga caaagccatg tacgatatga
     1681 aaagaaatac agaaccacaa cgcaaaaaaa acagtaaaaa tagtttataa ttaaccatat
     1741 gacacaacca caaggcaaag atactcgagt caaggctccg caaaaaccta tggtttcctt
     1801 acctcccaaa ccttttactc ccccattcaa caaatccttc ttcaatagta aacaacgcag
     1861 ttttagtact gccttcagaa cccaaagtaa aggcggcgga gggaaataat ataaaaaagc
     1921 tattttttct actatttcta gctatctctc taggaattgc caatcacaac ataatcaacg
     1981 tctcccaatt tggtaatgtc ttaggtgata gcactatcat ccttcccgac cacaacctaa
     2041 aacacattga agttgacctc accacccaac gtctccatgc ttttgagaat ggtcaaaaag
     2101 ttttcgagtt ccttatttct tcaggcactt ggaaccgtac ccctaatgga actttccata
     2161 tctgggccaa aataaaatct caaaaaatga gcggtggctc aagagaacaa ggcacctact
     2221 attatcttcc caatgtaccc aacattcttt ttttctacaa cgacaccacc aaaaaacaac
     2281 tcggctatag cattcatggc acttactggc acaacaactt tggtacaccc atgagtcatg
     2341 gttgcatcaa catgtctacc cccgaatcaa aacaaatatt cgactgggcc gaagttggca
     2401 cctcaatcac tatctacggc aaataccaag cacctgtatc ggaaaaataa gctatttttg
     2461 tggtacaatt gattaatgaa ttctcagttc tctgttggag actacatcgt cgattccgat
     2521 tccatctata tcatcactaa aattgatcaa gggcgaatat tctacagccc ggctaatacc
     2581 gaaggccatc accccagtgt cactggatca atcccattag acaatcttgt ctcttctgga
     2641 ttccgccccc tttgtgccaa aacagagatt gatcaattct tcaaagaact ttcccaagca
     2701 aaaaccggtg aaatcatcga ctcaaagctc cacaaagatc ttcgttgcct caatgagcca
     2761 accaagctca tcccccttct aaaacaactc tggactaaca aaaacaatcc cgacttaaac
     2821 ttttctggta gcaatcgtga cactctcgaa aaaatagtcg atcacttatc tcgtgaattt
     2881 gctctatcct taaaacaaga ccctgacaag attcgtaaaa aaatcaccga caccctcctt
     2941 aagaaataat ttgctaaaat acctctatct aaatgatctt ttgtcttaga atttagattt
     3001 taggattttt atactgggcc catagcgtag ttggtagcgc gtccccatgg catggggaag
     3061 gtcaggggtt cgagtcccct tgggtccacc acataataaa ttaaaaattt gaacttaggc
     3121 ccatagtgaa acggttatca ctgggatctc caaaatctcc attcgaggtt cgaatcctcg
     3181 tgggcctgct ctacgtctct gccagacttc ggcaggcaaa cctgccaaac aaaacacact
     3241 gaaactctca tccttgattt tttacccccc acctgaccat aatagaggac tatgacaata
     3301 acaaaagccg tcctagcttg cggtggttgg tccacccgtt ttctgcctac taccaaaagt
     3361 tacgccaagc aactcctccc tgtacttgaa aaacctcaaa tactttatgc cttggatgaa
     3421 tgcgtcagct ctggtatcga tgaaatctgc attattcatc gcgataaaga actaaccatc
     3481 aatcaatttc tcggaccaaa tgctgagtta gatgaatatc taagcgtcac caacaaacaa
     3541 aaatacatgg acgactacaa tcgtattgtc ggccaaatca aaaatttaat cttcctccct
     3601 cagacagaca atttccctta tggcaacggc tcccctgtcc tcgtcgccga acaatttatc
     3661 gaccgtcaac cttttgccta cttctatgct gatgatttta tattagaaga taaagctggt
     3721 gattatctca ctgctattat caactctttt aattccaatt ctgccgatgc tgtctgtgct
     3781 gtccagccga ctcctatgga agaaatccat ctttatagtt cagtcaaata ttcacaaggc
     3841 aatagaatgg aatcagtcat tgaaaagcct gaggcttcag ttgctccatc caatcacgtt
     3901 atgtttggcc gctttgcctt tagccccaaa attatcgaca tactcaaatc caccgccact
     3961 gctcgtggcg aactttggtt aactgatgct gttaacaatc ttgcttctaa tcctgaaaat
     4021 cttgttctta ccgaatccgt ccccgaaggt tcccgttgga tgaccaccgg tgaccccctc
     4081 aactggctca aagtaaacct tgctgttgct ctaaaacacc ccgacgttgg accaaagatc
     4141 acccaattca tcaacgaaat aaaataggcc aaacacaacg aatacgcacc actgcttgac
     4201 attataggac ttatagtcta tactataggt cagaatagat tgttgatttt gataatcgac
     4261 tttcgatttg tttaactgaa tttttcaaaa accctttagc tctttgtagc taaaggagaa
     4321 tatggagtta ggtggctcct cgtcatttct aaccccatat ctcctttggc tataagccaa
     4381 aatagtaagc accaaacttt aagcaaagcg cttaaaaatt tagagcttgc taagtacttt
     4441 aacaacttgc ttgctttata aatctttttc tcaaccggct cggctcgggc agttttcatc
     4501 tatctcctat ctagatcatt tggtagcaat accattttga acatcaatca ctggtgttta
     4561 ggatggtatt gctactactt tttagcgaac aaacctaagt tcacccatgc ccctttgggg
     4621 tactgacccg cctgatcagc gcggtctttt actgcatatg cagaaaggag atctccatga
     4681 acgaagactt cacccccgtt acaaccgtac gcaccaattg gaccccctgg attttgggta
     4741 tccttttggc aatcatttct tgctgctgcc tgctcggcat cgtaactgtg gcctatactt
     4801 tctggcccac cgccgaagcc gccgcccagc ccgccgccga agccgccacc cagcctgaaa
     4861 tcgtctcatc cgcacctgaa tatgcccctg actcaacggg cgtcacttct tgtaatggat
     4921 gggatacatc acctggcgca accaacaccc ttctgcctgg acagacagtc cgcggcgacg
     4981 tcgttatcaa cggcaaagcc tactacgaca acggtgtcgg cgaaggtaca tctggtatca
     5041 acatgaccag tgatgggttg gaagtctact cccagtgggg atcggggtgt gaagacacta
     5101 ctgatatcta ctatctggtc gagaaagacc ttaccaacgg ctgtggttcc gagtgtgatg
     5161 tggcacgtat tgtcatcttt accgacaatg gcattagcca gaaattccac actgaaccgt
     5221 agcccggcgc gcgcatccct gtgggtcctg gcttcggcca ggacccacac caagggagag
     5281 aaaaagagaa aagcaagcaa gttacgttat agggaatata ccctactgat gaggtccagt
     5341 tgcaacaccg gaccgaaaac gttatcttac tctcattaaa taaactgatt ccccgctagg
     5401 taagtaatac tccctaatct tttcaaacct ttccggattt tgccagataa aatctctagt
     5461 ctgtttcaaa tattctaagt tagtcgcata aaatggcgcc ggctcaaaat ttttcccacc
     5521 caccaacact gcctcaaaat tctgccaatc attaaagtta ggcacataca ctgaatgcat
     5581 ctctaacaca ctatcaccct gctcaacaag catctctcga tacattttca aattattgtc
     5641 atttatttct gcggtgttaa ccaacactaa taccttacca atttccagtt caaccatttt
     5701 ctcagctatt ttctcagtcg gccacactac tctgcttgcc gatctaactg gatagtccgt
     5761 aaagttaacc caatctatat cccccactag aaaagttggc gtaaccattt gcaccggttt
     5821 tctaaatggc caaccaaaag agttttcaac aaaattgaaa aataaataaa caataaaaat
     5881 ataagccacc cacttatatt taatttttaa caaccaatct cccaaccaca aagccaccac
     5941 cggtaacaac ggcatagtaa acctaaaatc cttatttctt ataatcgtaa aaattacata
     6001 tgttgctata gtccaaaaaa ttaatctttt atttttttca ttcctttcta taccaaacca
     6061 agaaacaact gccaatatcg ccacaatcgg ccccacttga tgtaaaaaga aaagcctaaa
     6121 ataatgtatc caagtaaccc aactcattaa attcatcgga tcagttaacg cttcccctgt
     6181 ccccgccaat ccagttagat attgataaat attttggaag tttacccccc accaccaccc
     6241 cacacacacc aaataaacca atccacccat taccatcctc aaccagaaat caacatcctt
     6301 tcggttctcc cacaaaagaa atagaaatat tggtacaaaa tacaaaaaac cgttcaattt
     6361 agtcaagctt gccaaaatca acatcaacca caaccaatta tctttccctc taatccaaaa
     6421 aagtaaaccc caaataacaa aaaccattaa tggtaaatcc aacaaaaaac ttcgagaaat
     6481 atcataaacc accgggaata acgaaaacag tattgccggt agtaatcctc ccaaaaagta
     6541 cactccgata actgccaata ttaaaaacac actatttagc aatgtaattg tcatcactcc
     6601 aaaaacattc atccataccc caccaataaa ataaatcaat ggcggatagc ctccaaacga
     6661 tctcaccaat tcaataaatt ttcctccctg gcttgccagt attacagatc gaatatgcgc
     6721 cgattgatcc cacgccggcg gcgtcttatc aatcaacaac cacaccacgt tcacccccac
     6781 caaaaataac agccacaccc acaacttttt catcatttta gtataaacta tttatagtga
     6841 aaaaaatcct gtcctcacca ctattttatc ccattctttt aattttaatt tgtcttttaa
     6901 ttgcctttaa aaactacacc ccaggaactt ttttaaccgg ctgggacacg ctccaccccg
     6961 agttcaattt cccccttaat tttcaacgac tattttttgg tgtctggcga gatgaacaag
     7021 gtctaggagc ggtatccggt cattcagcca tggctgatct tcctcgcgta tttattctct
     7081 ggctatttca cttcattctt cctctctcat ttctccgcta ctcctacata tttctatgtt
     7141 taatcgtagg tccaattggt ttttattctt taattaaata tttatttaaa aaacccaccc
     7201 ttgccttcct tggtgctcta atctatttat tcaacttagg cactctgcaa cagttttacg
     7261 ttccttttga aatgttcccc acccaatggg cctatctccc ctggataatt ttgtattccc
     7321 taaaatatct tcaatctcct tcaactttaa gacttttgac tttcggactt ttgactttac
     7381 tagccacccc ccaagcctac gctgcccacc tatggtatcc atttttcgga atatatattc
     7441 tattcctgtc aacccatacc tttctaaata aacacaaaat caagcactca ctaatactag
     7501 tattactaac tctactaatt aactctttct ggcttctacc taatatttat tacgctttat
     7561 ctcaatcaga aaacccacgc tacaatctat ccaaccgcct ccattctcaa gaatttctcc
     7621 tcaaaaatcg ccagactgga accatagctg acacttccct aattaaaggg ttctatttta
     7681 actgggataa atataatttt gaccaacgtt catccgaacc attaatgcca caatggaaaa
     7741 gccatcttca aaattacgat atccgactta ttggagaaat catttttcta agtgctttca
     7801 ttggacttat ctatgccttt atctccaaaa acacagtcct tattcctttc tctccatttt
     7861 ttattattcc cttcatcctc ttagccaaca acacaccaat tttcaaaaat ctatttgacc
     7921 tgcttctcaa aataaacctg ctagaagaaa tatttcgttt tatattcagc aagctttccg
     7981 tattgttaac ctttggttat tcgatttttc tcaccttctt tatatctact attttcatca
     8041 ttatccccaa agtcaccaaa gctttatcaa taagcataat catcggactt tttatctatt
     8101 gcttccccta cttccaagga gatctcatct ctagtttagt tcgcaccaac atccctccat
     8161 catattttga agtccaaaaa tatcttcaag acaaacactc ctctagaata cttactctac
     8221 ctctccatga acctagcggc tggacctatt attcctggaa gtatcaaggc tctggttttg
     8281 tctggtttgg gctaaatcaa cccgtatttg accgtgattc cgaccgctgg gcctatcaga
     8341 acgaagaggc ttatcgtgaa ttcaagtact cactttattc aaaagacatc tctcaactca
     8401 ccaacactct aaaaaaatac cgtgtaggct acatactctg ggactcttct gttgtatcac
     8461 ctcagcccaa aaacaatgat caaattactt ttaaaaacga aactaatcag ctactctctt
     8521 tgttagaaga gtccggatat ataagcctca ataaacattt tggtactata aaaatatttt
     8581 ccgttaacac taacaccaat cttattgaac taaaaagaat caacaacttt atcggacctt
     8641 catatcggtg gcattatttt gattatcaaa acaaagatga ctatctaacc acaaacacct
     8701 caaaagatag tatcttcccc tttcggtcaa ttcttgacaa tctgaacaaa gccaaaccag
     8761 agatactctc tcaactgaac ataaatcaac aaccagaaac tattttttct ggcacaaaaa
     8821 attttgtcag tcttaacaca acccaagggg caggtataaa tcttccaaac ttaagccaca
     8881 accactggta cattatcggc atcaagtcaa agtacgattc cggtatccct ttacgtctat
     8941 gtctaaaaaa tcaactcacc tctttatgta caatcgaaga cgaggttacc aaaaacaatc
     9001 aatctgattg ggactatttt cttgttccac cacaagatga gtttcttgga tacactgtgg
     9061 agcttaatct aatctcttac ggcagcaagc agtcatcctc aacgttagag acagtcacta
     9121 tatctcaaat tgacaaatca gtgattgatg ccagatttga acccacacct tcagttaatt
     9181 acccactcaa gttcaataaa atatttccca ataattctct tatccgactt aaactaaacc
     9241 aactagaatc caatcagacc atcattctca accaatcctt ctcccccggc tggctcgctt
     9301 tttattttga aggtcttcgt cctatttttc tcaaagatca cattctcgtc aacaactggg
     9361 ccaacggttg gcaactgaca tctgaaaaac tgacatctga gatctacatc ctcttttggc
     9421 ctcaacttct ccaatttctc ggctttggat tattaatctc cagctttatt tttatttttc
     9481 gtaaatcgga aacttcccac acaacttctc caccatctgc ttaatctctt ttaatctctg
     9541 attgccctca atctcactca taaacttttt taaatcaact tttctttctt ctgcttcagc
     9601 tgacacactt tgcttgccga cgatatcaat aacctctagt atccattttc caatctgttc
     9661 catctcccct tccctcatcc ccctcaccgt caacgccggt gtccctagtc taattcctga
     9721 aggataaaaa ggtgaagctg tttcatttgg tacagtattg cggttcatta ttatccctgc
     9781 tttttccatt gcccacgccg taaaccaacc agattgctcc ttgtctctca aatccactac
     9841 tatcaaatgc ttatccgtcc ccaaagacac cacatccaat cctcctttta ccaaaatttc
     9901 cgataatttt tgcgcatttt ttactgtttg ttttgcataa tttaaaaact catccgtctt
     9961 tgtcttctcc aaagccacgg ctattcccgc aattgaatgc aaatgtggtc caccctgaag
    10021 ccctggcatt attgccatat ctaactgttg ccccagttct tttttacaaa taattattgc
    10081 tcctctcggg cctcgcaaag tcttgtgtgt tgtcatcgtc actatatcag catattcaaa
    10141 aggagattgg cacactcctg cagcaatcaa tcccgcctca tgagccacat ccgccaaata
    10201 atacgctccc acttttttag caatttccga cattctctta taatcgatct cccgaggata
    10261 cgccgtcccc ccagaaatta ttagctttgg tctaaaaacc aaagcctgtt tttcaatctg
    10321 ttcataatca aataatcttg tctctggatt gactttataa aattccacct caaacatttt
    10381 actaacaaaa gttgttttct tatctcccat tctccagcca tgcgacagat gaccgccatc
    10441 gggcaaatac atcgacatta tcttatcgcc tgcatttaat aaagcattgt agactcccaa
    10501 atttgcctcc gacccagaat gagcttgaac attggcatgg aatttttccg gatcaagatt
    10561 aaaggctttt agcgctctat ttctagccaa ctcttcaatc tcatcaacta tctcatttcc
    10621 ctgataatac cttcttcctg gataaccctc agcatatttt tgcatcaata ttgaccccac
    10681 tgcctccaac acttccggat aagtatagtt ttccgaagga atcatcatca aagaattttg
    10741 ctgtctttgt ttctctttct caatcaattc aaaaatttta tccatgccct attttactct
    10801 atttacctca caacctcttc tctttatctt cccaagtaaa caattccccc agagacctct
    10861 ctttattaat tcttttaatt actttagcca aaagtggagc caccgagata acttccattt
    10921 tcttacaaat tttttccact ggtattggca aagtgtcggt caatattaat ctatccacac
    10981 tactagtatc aaacttttct ttaacatcac ccgacaacac cccatgagtc gcacaaacta
    11041 ttatttccgt tgccccaaat tttttaagaa cctccgccgc cgcacaaatc gacccgcctg
    11101 tatcaatcat gtcatcaatc ataattgctc tcttacctgc cacatccccc accacatgag
    11161 ctacctcagc ttgattgtga gcttttctaa ccttatcgat aattgcaata tttaaaccaa
    11221 ataggtccgc caatttatta gcccttttca ctcccccaat atccgggcta actatcaccg
    11281 aattcttaat caaccttttc ctcttcaaat atttagctaa aagcggataa cccacaaaat
    11341 ggtctactgg tatatcgtaa aaaccctgaa tttgatctgc atgcaaatca attgtaatta
    11401 atctatctgc tccagcctta gtaatcaaat tagctaccaa cttagccgtt atcgcttctc
    11461 tcgaatccac tttacgatcc tgtcggctat aacacatgtg tggacatatt aaattaattc
    11521 ttcctgccga agctcttttc agagcatcaa ttaatatcaa catctccatc aaccgatcat
    11581 tattttctgt cccaaaactt tgaattatat aaacatcatt acctcttact ttttctttta
    11641 ttcgaacata tacctcccca ttagcaaaac acttatcttc caccggtgtt aatgggactt
    11701 taagaatatt agatatatcc ttagctaagg ctatatgtga cttaccagca attaattgaa
    11761 gtttggccat tttattttac tgaccaattc tattccctaa aaattattct gacaatatat
    11821 caccttgaaa gaatcgtctt tctattctct ggcatactcc ccaactgagg tgacaattca
    11881 ccacgccttt tcctccacat gcaatcagtc ttaaaatcac atttaggtgt aacacatctc
    11941 gccaactcat taaccacatt acccgaaaaa cacctcgctc ttttgatatt taccaccacc
    12001 ggctctttct tctttttttc agccatatag taactatttc aattcaaaca caactactac
    12061 acttttagaa atagtcgagc tacctggctc aattggtgat ccaccaccac ccatgccatc
    12121 tttagcggca tagatcggaa ggttagatac cgatccacct tcatttactg acatcacttt
    12181 acccaaactt cttcctgaag ctttggcaat tgcttccgct ttttctctag cgtttttaat
    12241 tgcctcatca aatagctcag tctccacttt attactattg tccatttgaa aattaggtcc
    12301 ataaacgttg ttagcaccag aagcatttaa aagatctgtt agcgccgaag tcttatcaac
    12361 ctctcgaagc actatctcaa ttgaattact tactctccat tgacctttac gagatttttg
    12421 caccccattg tcccaataca tttcttctga ttgatagacc gaagtatttt gagtttttat
    12481 atcttctgtt ttaataccaa accccttaac tgcactgatt aaagcctgga ctttatcatt
    12541 tacttctttt acagcatctt cttttttatc atttaccgac tccactccag cagtaaaact
    12601 ggccatttga tttttctcaa tctttcgagc ctcaccagtt actgtaatta tctctcctgg
    12661 ctgcatcgtc accctccccc agttcatttt
//
LOCUS       DDWP01000051            4627 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_44268, whole genome shotgun sequence.
ACCESSION   DDWP01000051 DDWP01000000
VERSION     DDWP01000051.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_44268"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1448 a         1048 c          795 g         1336 t
ORIGIN      
        1 tttcttcttg ataaagactt taccgatcca tccgaaacat tcgatgatcg tcttgcccat
       61 cttcgcccca tttctcgtac tgaagcttat caagctgatg ttacttatgg caccaacaac
      121 gaatttggtt ttgactatct tcgtgaccat atggttggtg acatctctga aaaagtccaa
      181 agaaattacc actatttttc tattgtcgac gaagtcgatt ttgtcttaat cgacgaggcc
      241 aggactcctc taattatttc ttcgcccaac aatcagccag ttgataaata ctacaaattt
      301 gccgaaatag caaaaggtct acaaacttcc gactacacta tcgacgaaaa agcccgctca
      361 gcttccttaa ctgaatttgg tatccgtaaa gtagagaaaa ctcttaatgt cggcaatctt
      421 tacgaagaat cttttgagac cattcattac attgaaaatg ctatcaaggc tagagccatt
      481 tttcataaag acaaagatta tgttattaaa gacggtgaga taatcattgt cgacgaattt
      541 actggcagat tgatgcctgg acgtcgatgg tctgacggct tacatcaagc tgtcgaagcc
      601 aaagaaggtg taactgtcca gaaagaatct cagacctggg ctactatcac tttccaaaac
      661 tatttccgtc tctacgacaa gcttgccggt atgaccggga ctgctgctac tgaggcggaa
      721 gagtttagaa agatttataa cttagatgtt atcaccatcc caacttaccg cgaaataagg
      781 cgccaagatg aaaacgactt tatcttcaaa actcaacgag ccaaatatgc tgccattgcc
      841 gccaaggctg ccgaacttca caatttaggt caacctgttc ttatcggtac caccagcatc
      901 gagaaaaacg aaattgtctc ttcaatgctt cgcaaacacg gagttccttt ccagatgctc
      961 aatgccaaga accatcgttc cgaagcagaa ataattgcca aagccggtaa ggttggtgct
     1021 gttactgtcg ctaccaacat ggctggtaga ggagttgaca ttatcttggg cggacctaaa
     1081 gaaggggtgg ataaaaaaca atggcaaaaa gatcacgata aagttgttga gttaggggga
     1141 ctgtacgtta ttggtactga acgtcacgag tctcgccgta ttgacaatca gcttcgtgga
     1201 cgcgctgggc gccagggtga cccaggtttc tcccaattct atgtcgccct cgacgacgat
     1261 cttatgcgta tctttggtgg tgagaagata tcttctctca tgactagatt caatatgccc
     1321 gacgatgagg cattaactca cccaatagtt tccaaagtta tcgaacaaat acaagtcaaa
     1381 gttgagggtt acaattttga tattcgtaaa aatctagttg aatatgacga tgtgattaat
     1441 aagcagagaa aaataatcta taatcttcgc gatcaaactc taattaatag taaagacagc
     1501 cctgagaaaa atttggctga aattaataga aaccttgaag cccaggttaa ctccatagtt
     1561 gctctcaacc acaaccccga aactgatgaa attgactatc aaaaaatcgt tctagagttt
     1621 tctgaaatac tacccatggc cgagactgac cgacaaaaac tcaaagccga tctggaaaag
     1681 tccgatgatc cctcaatctt cctttttgat ttgctaaaaa aacaatggca acaaagatca
     1741 gattattttg gccaagctat ttcaagtaat gtcctagcct attctattgt caaaacactc
     1801 gatcctcttt gggttgaaca tcttactgcc ctggatgatt tacgtgacgg agtccgtctc
     1861 cgaggttacg cacagaaaga tcccttggtt gaatatcgta aagaaggcta tgagatgttc
     1921 caaaaattaa tggagcaata tcaatacaat ctagcccgac tgttgttccg tcttgagcca
     1981 gttgtcgcca atcctatcac tcaacctcaa aacgctaccg aaagcagggg agaattacca
     2041 tcagaagaca aacccaagaa ggtcagtaca atcaaaaaca accagacttt aggtagaaat
     2101 gatctctgtt ggtgtggcag tggtaaaaag ttcaaaaaat gccactatcc agctataagt
     2161 tgatatcttt caatatcttc tcatatcttc taatatattc taatgatttc ctatcacttt
     2221 agtgaaacta aagacatcaa agaatatcta ataagtattg aagcaatcaa actaagtttc
     2281 gaagcttcaa agatccttcc tcagcttgaa gaaaaaattc tccgccagtc aatcttacgc
     2341 agctctgtct tttcagctcg tatcgagggt aatcctttga atatgtctaa ctacgacaaa
     2401 ttcgacgacg aacaagttca caaaattgaa attaacaact tattaaaatc atatagacac
     2461 ctatactctg ccactaaacc acccagtttt agcttggaaa ctattaaatc tctgcaccaa
     2521 gatgtcatgc gtaacttatc acccatggcc ggtaagttta gacaagaacc ttgggctatt
     2581 tttgacagta gcggaaatgt aattcatctg gctccgacct atcttaagct tccagaatta
     2641 atgtcaggat acatcgagta catcaacgga cttgactgtc atccgacaat aaggtcagcc
     2701 atagcccaat ttgtctttga aaaaatccat ccttttgcag acggcaatgg tcgtgccggt
     2761 cgtctaattt ctgcactaat tctaaaacag cacaatttcc atcttcgagg catgattcct
     2821 tttgaggaat acaccgatac tcatcgagaa gcctactatt atgctctcga accaagcaac
     2881 gatatgaccg agttcattga atactatttg aagtctctag ttttcactgg tcagaaaata
     2941 ttgaaacttg tcaaaagtaa ccctgattct gtacctggat tacctcctcg ccgccaagag
     3001 atattagaaa ttatcatcga ccaccccaat tgtacttttg actttcttgc tcgtcgtttt
     3061 gcccaagtta acccaaaaac tcttcattat gaccttggcc aactccaaaa gtctggactt
     3121 atccgcaagc tcggcgccac ccgtggtgtt ttatacaccg cagtctaata cttgcatatc
     3181 taatgtgtat atcttataat acacacatgt ctataaaaag aatagatcac tcgctagaat
     3241 ctcaaagaaa tacaaagttc aaaccataca acatcgaaaa caggctacta aaagtcgggg
     3301 acgatctatc ccagcaacca atagaccctg aagctgtcga gatacctaaa gcccgattac
     3361 cgatggctag acttgcagta gcaaaaacat tggtatatta tggatactct gccgaagatg
     3421 ctgctagagc agtcaactta ttagaagacg gtgaaccttg tccagatgca tacaaaagca
     3481 aacgtgttac tccagatcaa tatttaggtt ggtaaaaaag ttttctactt aaatatcgtc
     3541 tctcggtaaa acttcagctc gttaattgaa tacttaatat catctaatgc tcggtgagac
     3601 acatcttctt ccttatgtga caaaaatact tttgcctctt tggcatacca tcttttcgcc
     3661 aattctttaa cagaagttac atcaatcatc cggtagttaa ggtaagaatc tactctagga
     3721 aaatattttt tcaaaaattt cctatcagca tgtatagagt taccacacag aagtcccgtc
     3781 ttcttttcac aatacttcct aaccatcttc aatacttctc tttttgcttc actcaaacta
     3841 atttttgatt ccaaacactt tttagtcaag cctgacttac catgttgttt tttgcaccat
     3901 gagttcattg aatttaatac ggcacttggc tgatgaatca ccatatcaag tgactcatct
     3961 aatatattca actcgctgtc agtcactacc accgctatct ccaacactct atcaacctct
     4021 ggatcaagtc cagtcatttc catatccatc caaattaaat taagagacgt attcatttag
     4081 ctattataac tgatattatt agatcatgaa tgactgtatc ttttgtaaaa tcgttaaagg
     4141 tgaacttcct tgttataaag tctacgaaga tgacaaatat ctcggctttc ttgatatcca
     4201 tcccctaact ctcggtaatt ctcttttaat ccccaaagat catcatcgtt gggtctttga
     4261 tgttcccgac tttggcacat attttgaaat agccaaaaaa atatcactgg cttcccaagc
     4321 tgtcctcgaa gccgaatctg tctctcttct aactcttggt tacgaagtac ctcatgccca
     4381 cattcgcatt atccctcgct acgctaacga tcttcacccc aagggtatcg atacaagcgt
     4441 agatgtaaaa actactcctg agcttatgtt aaaaatttct acagatatct tatgtcagac
     4501 caagaaatat taatcgaatt gcaaaagatc aaccaacaac tcgccaagag taacaccaaa
     4561 agaactgttt tcaacagttt tctagctgga ttctttcact catttggcag ctttatcggt
     4621 accgcgg
//
LOCUS       DDWP01000052            3051 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_39951, whole genome shotgun sequence.
ACCESSION   DDWP01000052 DDWP01000000
VERSION     DDWP01000052.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_39951"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1145 a          382 c          721 g          803 t
ORIGIN      
        1 cggacaggct ggtttaagaa agtggtcaga acaaaagtag gttcacctca tattgtaggg
       61 gcgatggatg attttgaaaa agagggtttt aagaaagtca tgggatatga attgaatggt
      121 ggttttttta tggacgagct taagacaaga gatgcgttaa tgccgatatt agggacaatt
      181 gctttagcaa aaaaaatgaa taaaactgta gctgaattaa tattagagct accccagagg
      241 tttacttata gcttaagtat aaaaggaaca ccgaccgaac tgagcttaaa aataattgat
      301 gaatggaaaa aatataaaaa tgatattgag gtggaatttg gaaaaataaa aaatattgac
      361 ttactagatg gactgaggct gacattcgaa aatgatgata tcgtccattt cagaccatca
      421 aagaatgccc ctgaatttag aaattatact gaatcagaga cttatgggaa agcaaggatg
      481 ttgtcagaaa aagcaattaa tttgattcaa aagtggacta agtagggtaa aatacaaata
      541 taaaagaaga ggattggaat aaagaaccag ttaagttaat tgtgaaaaac atgggtggtt
      601 taacacctgg ggaaaaagtg attatgcaaa gggttgtgac tatatatcag ggtgatccgg
      661 caaacgcagc tgacttggtg cgaaaagata tccgtgatct ggcaagtggg tcatggaagt
      721 gggcagggag agatggggtc aaaacggcaa tagcgggaat caggactgtg gttagtgagg
      781 aatttgtacc gagaaattac agaagataag tgtggtaaaa tgggtcaatg ctacgaagcc
      841 tgtcgcgaag gtggagctac tttagaaata aagatcggag agaaccgagt aaactcaaaa
      901 aagtataaat acaaggtggt accacgagac aaaagtcccg tccttcggct taataattat
      961 taaaccctaa gacgggattt ttatttataa taagattatg aaattcaaaa ttgataagaa
     1021 tgtctttgac aagtttcctg atttagtggt agcaatacca attatctatg gatttgataa
     1081 caataagtca aaaactgagt cagaaaaaat tttaagagat gccgaagata atttaagaaa
     1141 taaatttaca cttgaatcat tgattgccga caatcgagcc agaaagtata tggattgttt
     1201 cgttaaattt ggttgtgatc cagatatctt tacaccagca catattgcat tattaaaaag
     1261 agtaattgag aacggacaga taccaagtat taacccgatg gttaatttgt acaatgcttt
     1321 tagtatcacc aacatgactc cgtttggtgg ggaagatttg gataaagtgt gtggagactt
     1381 taaattattt tttgctgaag gtggagaaaa gtggtatccg ataggaagta aaaaaagcaa
     1441 accagcagtc gaaggtgaat tagtgtgggg agatgagcta aatttatcaa caagatcttt
     1501 gaattggcga caatgcgaaa gaacaaagct tacatcaaac tcgaaaaatg gctattttgt
     1561 aatggatggt tttcgggggg taaatgatga cctaataaag aaagctgcaa atgaatttct
     1621 aaagcaggca acagagatgt ttggtggtga atcagaaatc ttgtggttag acgctaagaa
     1681 tacggaagta gaaattagtt ttgtaagtaa aagcaagaaa gattttgaag taataaaagt
     1741 agagagaaag aataacaaaa aagttgaaac agggattgct ggagagataa aaaagatgtt
     1801 acttgaagtg ctggatgcta ataactcatc tgagttttca gtagaccatc cggcaaacac
     1861 acagtttgga gattattcaa ctaatgtggc aatgattctg tctaaagttg agaaaaagaa
     1921 tccaaatgaa attgccaaag gtattgcgag cttaattaaa ccaagtgaca taattgaaaa
     1981 ggtagaagtg gtgggaggat ttatcaactt ctacctcaag aaaaatgtac ttaaagaagg
     2041 tgcagaaaag ataaattacg aaattgaatt caagaacaaa gttggagaat tcctaaagaa
     2101 taaaaaaata atggtggaat ttgcacatcc taatactcac aaagaactgc atattggaca
     2161 tatgaggacg ttaattacag gagaatcatt agctagagtc ttggagtttg tgggagctaa
     2221 tgtgtttaga gctaactatc agggagatat tggtccacat gtggctaaag caatttgggg
     2281 aacccgaaaa ctattgtcac aaagcaacaa gagttttaag gactgggaca aggagagctt
     2341 tggtgagaaa tcacatttcc taggaaaggg ttatgcactt ggttgtgcag aatatgaaga
     2401 taataaggac gaaattgaca aattaaatca atctctatat gaaaaagaat tgtcagtaat
     2461 gccagactac caattaacta gacaatggag ccttgattat tataatgagt tttatgaaag
     2521 atttggtact agatttgaca aattgttctt cgaaagcgag atagccaata aaggtaagga
     2581 aattgtcgaa tctttggtcg gaactatatt agaaaaaagt gaaggagcaa ttgtatttga
     2641 tggtgaaaag tatgggttac acaaaagagt atttgaaaca gcactaggaa caccaacata
     2701 tgaaggtaaa gaattgggac tagcttacgc ccaaagagag gtatttgaat ttgataaaaa
     2761 tatccacgta gtggctaatg aacaggcagg atattttcaa gtggtaatca aagcgataga
     2821 gttgatggat gagtggttta aagacagaga acaccactta tcaatgggaa tggtgacttt
     2881 ggttggaaga aagatgtcat ctcgaactgg agatgtggtg acagtcgata gcgtgcttga
     2941 cgaagttaaa gaatcgatta gaccgttcat caagacggaa ggtatgacca aagatgcaat
     3001 tgaagaaatt gctgaggtat cgacaattgg agctgtaaag ttctctatac t
//
LOCUS       DDWP01000053           22535 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_22167, whole genome shotgun sequence.
ACCESSION   DDWP01000053 DDWP01000000
VERSION     DDWP01000053.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 22535)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22535)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22535
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_22167"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         6611 a         5886 c         3607 g         6431 t
ORIGIN      
        1 aacggcttaa gtctgacagg aaatgtcctt agccttggtg gcacactcac ccaagatact
       61 cgtctaaata tcggcaacac cgaagttatg ttctttaagt attcaaatgg aaatgtcggt
      121 attgggacta ctaacccatc agaaaaacta gaccttggaa gcggtaattt aacaaccact
      181 ggacaactca tttcaaaaag cggatcacac ggactaagta tccgtaattc tggaaacagt
      241 gaaattattt atactaatct caatggtggt agtggcgaac ttagcatcaa aaactcatct
      301 ttctcagagc gagtaaaact aaattcaaac ggcaatagct acttcaacgg aggcaatctc
      361 ggtgttggca ccggtagccc ttcttacaaa ttagacgtcc agagcacgtc caccacaagt
      421 tctgatgtct tacatataga aaacaacgtc tccaccgacg aaagttacgc aggattatta
      481 ttcaacgtag ggtctggaac aggatccaat caagccagaa tcgccgccgt caaaaaaacc
      541 ggaacatata gccctaccga tctagctttt tacatccacc cagatagccc aacggcaatg
      601 gtcgaagcag tgaggataaa aaataatggc tatctcggta tcggcaccac cgctcctgag
      661 gcaactctcg gaattcaagg taattttggc tcaaatacta acattggttt agcattaaga
      721 ggcaccaaca gtaatggtgc cgtaatctct ctcattcaaa gctcagttca atcttggggt
      781 attggcatgg ggtcgggtaa tactagacta tctttcttta acaatcgtta ccccggaaat
      841 gctggtactg aagtacttgc aatttctggc acaggaggtc tatctctagg ttattcttat
      901 gccaccgtcg accctggagc tggcaatatg attatctctg gcaatgttgg tatcggtacc
      961 accaaccctg cggtcaaact agaagttgcc ggtgatatta atctcactga caacaccaac
     1021 ggaacacgtc ttactattgg aggcaaatcc ggtactgctt ggccatacta cattgatact
     1081 aaagcaggag attatctaca aattctatcc gcaaattctg gagcaagccc atttgttata
     1141 gactcgaatg gtaacatagg tatcggtaat accaacccca acaccaaact ttcaatatcc
     1201 aataacgttg ccgctggctt tcttgatact tactctgaat atcaactcag tctttatgac
     1261 ggcgcttccg caagttctag ctacggtctt ggtgtcaaag gtaatactct tgttttaaat
     1321 agtggtgctg gagcctacag cttcgaccgc agtggtaatg ccactaccat gtatatgaat
     1381 acatctggta atgtcggtat cggtaccacc gctcctaatg gcaaactcga catccaagct
     1441 actaccgatc aaaatctttt aattcgcggc aaccttaact tcactgacgg agtcagtcta
     1501 tactccgtca atactgccaa cagtgcagtc cgcagcatgg agtttggtgc tagcaaatac
     1561 tattttgctg gaggcaatgt tggtattggt aataccaacc cagccgaaaa acttcatgtt
     1621 gaaggatatt taagatccgg aaacgcttac tacggcatgt ctggtcaagt tgccaacgat
     1681 tacaatatcg tctcgaatgg agatcaagca tatcgtgtag gacttggtac caacaatgga
     1741 acaggaaaca tagtatttaa aacagccgcc tttactgttg gcgacacaga aagaatgagg
     1801 attagatatg acggcaatgt tggtattggc aacaccaatc cagctaacaa acttgatgtc
     1861 gtcaccagtg ccaacgatgc cattaaggga accaacacta gcactggagc tggcgttatt
     1921 ggtgtcaaca ccagtagtgg aaccgccgtc tatgcctgga acaccagcac tggttatgcc
     1981 ctctactgtc aagccacagc tgccaataag tgcggtggta accaagcctg gtacaatgcc
     2041 tccgatcgtc gtcttaaaga aaatatcgct ccaatcacca attctcttaa caaagtcatg
     2101 gggctccaag gagtcaccta caacatgatc aacgatccca gcaagactcc cgtcatgggt
     2161 ttcatcgccc aagatgctct accttacgcc cctgaagtcg tcggtctcga ttccgaaagt
     2221 ggttattatg gtatgcaaac cagccaactt accgcattat tagttaatgc cacccaagag
     2281 ctcaaccaca aactcaccgg cctctccatc accaccgaag gtcaagttaa cgtcgactac
     2341 aatatttccg aagagattct cgcctctctt ggatacgttg attccaaaaa tgaaatcgag
     2401 tcagctattt attctcacaa aatcaacacc ggctttatta aaactatcaa cctagttgct
     2461 caaaacatta ttgctgaaac caccaaatca aaagttgtca aaaccgacac cattgctcct
     2521 cttgacgaca ttactgacac catcgtcatt gacggcaaca tcaaagccaa agaagcttct
     2581 ttctccactg tctatgctga caaagttatc agcaaagaag gtgaattcgg acaaataatg
     2641 actgacaaaa tcactgctct tcgtgacgaa atccgtgccg ccatcgcttc gactgaaact
     2701 ccacaagacg caacgccttc ggctattctg gctgaatcat cgggctggac ttatgacatt
     2761 gccacttccc aagtcactgt taccggcgat atgtccctca ccgacaacat ggttatcggt
     2821 tccaaactga tcgttgttgg cgacactcaa cttggcaacg cctttgtcac cggtaccttt
     2881 accgctggcg agattgctat aaaagacaat ttcatcgaga ccaccaatac cgctctctat
     2941 atccaaccca gtggtcttgg ctcagttaac attatgaata attccctagt tatcgctgaa
     3001 aatggtgaag taacccttag tggcaacctc actgtcggtg gtaatgtcgg agtctctggt
     3061 tctctctttg cctctcttat aaatgccgac gaaataaaca ccaagaagat taatgtcgcc
     3121 accgacagcg cctctgctcc aattatcgcc gaatccggct ttgcccaact cgctaccagc
     3181 tccgcccaag ttgactccaa tgctgctgct ggtattgcca ctctccctgc tggcaaaacc
     3241 gaactcatta tcaacaacag caagatcact cccacttcta ttatttacct caccccggct
     3301 ggttccacca acaatcaagt tgtttttgtc aaatcaaaat acatttgtga caagaacaat
     3361 cctgagcttg tcgaaggatc accttgcaat tcttccttca ctatcgctat cgacgaggct
     3421 ttgacccaag acatcgacat caactggtgg atcatcaact aaatctatat acttaaatta
     3481 tcaattttta tggttcggaa acgtctgaaa acagcactta gaaaaaacct cgcaaaatac
     3541 ctgttcttag gtattttctc tatatcatta ttatatccat tttttcctac ttctccccca
     3601 aaaaatacct ctcaaccaat caactttgag tcatctattt tttcctcaga aatcactagt
     3661 tccgtcaacg gtttttcaac ttctttaaat gttagttcca aaggagccga cgtctctatc
     3721 tctcgtgata aaagccagat aaaatttacc actcccctta ccaatgcctc aatttcccac
     3781 tccttaaatg aagttaccta tacagtaccc aacaacaata ttgagttacg atacaaatta
     3841 cttgaaaacg gcctaaaaga agacatcgtt ctccataaaa ttccccaaga aaatattttc
     3901 aaaagcaatc ttgttaccaa aaacttaact cttaaaaatt catcaaatgg tacccctgtt
     3961 ttctatgacc tcgatggtca atatcaattc catatccaag ccccatatgc cgttgatgga
     4021 caaggaaagg taacctacga tctaaagtat catcttatta acgacaaaga cgacattgat
     4081 aaatatattc ttgccctgga aattgacacc gattggttac atgacccttc tcggattctg
     4141 cccataacca ttgaccccac tatcgttcac gatactatct cagagttttc cggtggacaa
     4201 ttcaatcgtg ttaaagatat tggcaccggc gaaacagctc cagaactagt tgcttattac
     4261 caagaaaaca cctccgatgc ccacaccgtt ggcctctggc acataaacga aacttctggt
     4321 accaccgtca aggacagttc tggcaataac aacaacggca ccgccaccgg tactactatt
     4381 gtcgatggca tcctcgataa atcaagaaac ttcaatggcg gtaccgacaa catcaacctt
     4441 ggaaaccccg tctccctaca aatgactgga aatcaaacca tcgaaatgtg gctatacccc
     4501 accgattatt cagtcaggcg caatccttac gccaaagcct atggcggtga aggcaccatt
     4561 acccacgaaa caagtggagt cttaaattac tactacggta gcggcggcgg caatgcatca
     4621 ccatatacca gttttggaac aaaatatagc ctaccactca atcaatggtc gcacgttgct
     4681 atcgtcagag acatcaccaa tcaacagtta acttggtatg ttaacggcaa cataaccaat
     4741 caagtaggta acacccaaac cgcttcagta agctcactca acgcctacat cggccaagga
     4801 tacaccaaca actactcggg aaaaatagac gaagtccgca tttcgaatat tgcccgctct
     4861 ccggaagaaa taaaaatgag cgcctctcgt cgtccttaca gcgtctacac ctcccccacc
     4921 attgactaca ccgacgatgt ctttagctgg gactcttttt cttggacaga attaggagtc
     4981 gggaccagtg aaggcgaagc tctctacgat tctactggcc taatcgccca ctgggatttt
     5041 aacactctcg catcatctgt tcaaaatgtc ggaggtacct gtggctcaag ctgtaacggg
     5101 tctacttaca gtttcaacaa cgccggtaac cccgacgttc caccagtagc tgtcggcgga
     5161 accctttcag ttattgatgg ctacaacatc catacctttc gtggtggtac tcacagcctc
     5221 actctaactc acagcattgg ctctgttgaa atgctagttg tcagcggtgg aggtggaggc
     5281 gccaacactg gatctggtgg aggtgctggt ggatatcaac acagaatcaa atacgacggt
     5341 tttatatacg gagctggatc atatcctata tccgttggtg caggaggtat cggtggcact
     5401 gctacctcag ctagtggaac caatggagga gcttctcatc tcagagaacc aggcggagga
     5461 actatttttc aagttgtcgg tggtggaggt ggagtaagtc acggcggagc cgctggccaa
     5521 aacggagcca gtggaggcgg agggccaatt cgcactgcag cacccgcagc accaggtgga
     5581 ggaaattccg gaagcatagg cacctctggt ggtacagggt tggtcgatgt tggctgggtc
     5641 ggaaatagtg gcggtggagg aggtgctgga gctcaaggta gtaatggagg caacactgct
     5701 ggatcaggaa atggtggtat cggacttacc aacagtatta ccggcactaa tacttattat
     5761 gctggaggtg gaggtggtgg agaagttaac ggaaattttg tcggtgtcgg tggtagtggt
     5821 ggtggaggta gtgccgttct caatgcagcc ggaggaagtg gtaccgccaa cacaggaggt
     5881 ggtggtgcgg ggggtagcta taatggtagt tattttaatg gcggtaatgg tggatccggc
     5941 attactgtca tcaaatatcc caacacttca tcaggctgga ctactgatcg cagatgggga
     6001 caatatgcaa tgaaatttga cggaatcaac gatagtattg ttatccctga tcatccaagt
     6061 acaaacttct atcaaaccag tccttttacc attgaaacct ggatcaaact taattctctc
     6121 caagatcaag ttattattaa taagcaacgt aacactacca atgaatttat atatgctcta
     6181 cgtatcggaa cctctgggaa ggccacattt gaagtcagca aacagaatgt ctctagttct
     6241 actgttatgt ccaataattc acttgaaaca ggcaagtggt attttatcgc tgccaccagt
     6301 gatggtacca atatcaacct ttatctaaac ggcatcctcc aaaataccac cagtattgga
     6361 ttttcagcat caacccaatc acttgccgac atttacctag gtcaactata taacgatacc
     6421 caatatctaa atggtattct cgattcaacc cgtatctatt ctcgtaccct tcctccacat
     6481 gaaatcctca gcaactacaa ttccggtaac attgaatttc aaactcgagt cggcgatagc
     6541 tccgatccca acgacggcac ttgggacaac tggaatccaa tcactaacga agctattatc
     6601 cattcgcttg acaccgcccc cgatttctca cctgtctcct actggactct taatgaatct
     6661 gttggtacta gcattaccga ctctcgatcc accaatcatg gccgcacagt caacgccact
     6721 attactactg gcaaatttga catcggccgc tctctcgccg gtaccggtta tgtctacatc
     6781 ccccaccact cctcgatcag ttttaccggc aattttacta tctctgcctg ggtcaatccc
     6841 accactagtg gcaatcgtca aataatctcc aagaccggaa ctggtggcac tggcggtttc
     6901 gctatgcttg ttggcaatat gggggaagtc ttctgtcgaa cagacaacgg aattacttgg
     6961 acagattcat ctactgcctg gggagctctc cctagttcca ccggatggca ccacgtagct
     7021 gccgttcgca atggcactca gtgtcgcatc tacatagatg gtaccgatgt taccaatgtt
     7081 agtaataccc ataccactgc cacagctaac actcttcccc tagtcatcgg tgctcgatcc
     7141 gaccttagta ccgaattctg gaatggaggt atcgacgagg ttagagttta caataaagcc
     7201 ctctctgcca ctcaagtagc tgctctctac aacaccattc ccacctctct ttattattcc
     7261 ggttttacct ctgacccaaa tatcaaaata gaaggaacta attctctaaa atttcaaact
     7321 ggagccctta acgctgattc atctaccatt ggattatggc acctcgacga gaccagcggt
     7381 accggcgcct atatcaaaga cagtagtatc tacggcaacc acagcacccc caccggtacc
     7441 accacagtca aaggcataaa tgatcaaggg agaaatttca atggaaccac cgacaggata
     7501 agtgctagcg ataccggagc atcaggatca tttaccgttg aagcctggat taaaccaaat
     7561 actgtttcaa ctgctatggc cattgcggga gaaagatctg ctacgacccg aacctgggct
     7621 ttttatgtcc gccctacagg acaattaggg ttcaccttaa atggagtaac cgactatatt
     7681 tcaaccgccg ccggactagc agcaaatcaa tggcaacacg ttgctgccat aatcgatggt
     7741 accttcttga ggatctaccg caatggcgat ctgatataca gcacaaccat ggggaatact
     7801 gtagtaaatg atgcaacaac actcgggata ggctcagcca acggttccag tgcttcaggc
     7861 ccatttagcg gtattattga tgaagtacgc ctctctaata tagcccgttc tgccaatgaa
     7921 attgccgaaa gttaccgtct aggtcgccac cactacctca acatacccat caccagttcc
     7981 gatctttcga ataaattatc tctaccattc tatattgccg ccgaccgccc cggatcatat
     8041 ctttccgcca ctataggtga gtcaaattac atcaacggtc aaccagacaa caataccatt
     8101 ggattatgga gactcgatga cgataaatac ttggatagta gtggttctag caatcacgga
     8161 cttagtgagt attatggtat ggcttatgga atcagaagtt ctaaccttac ctttatcgcc
     8221 aaccagtgga acggatctgc caacgacggt gaacttgata taacctgcac tggtggcgat
     8281 gctgcttgct atattgtcac catagatggc attaccctct ctacccccac catcaactac
     8341 ggtgttgcta tgatcgaaaa tgcagcactt tcagcttttg ttatgtattc cccttctcta
     8401 tcagcacaag ggataactgc atatacaagc aacagtaacc attttattgg agttagatat
     8461 aacggcacca cttggcaata cgacaataat gtcagttggg taaactttac tcccaacagt
     8521 tccaacatta tagttgcttc attaacaacg ggcaatccga caatcaccag tctcatacaa
     8581 ctcaaccatc accccatatc cgctcccggc aagatcggca aatcccgata ttttaatggt
     8641 acaaactata ttgccagcaa cctttccagt gccgccaatg acctatttgc ccaatctgga
     8701 gtctcctgga cagttgacgc ctggttcaaa aatccaagtg ctaatgctac caatcaaatg
     8761 attgttggtc gtggcggagg taccggagga tcagctacat ttggaatata cataaacact
     8821 tcaggaaacc tcgtctctaa tttacgtgga gtcaacaccg tcctaactac tactaatgtg
     8881 tccgacaatc tttggcatta tatcgccatc acttgggacg ggacaaatgc ccaagcctat
     8941 ctagacggac aacaaatttc tacgcctggt gttggcaccg ctgccgtaca aacatacaac
     9001 gtctatatgg gggctaccgc caacggcatt tcaaacttct ttactggcta tatcgacgaa
     9061 atacatatca gtaatattgc gcgttctgcc gaagaaattc gtcaagccta cagtattagt
     9121 acccgcaccc atcaaattac tatcgacttc gccgccaaac tagttagcag caacttagtt
     9181 gtcggctcca cagattattc ttttaatata gatactattg cttatggctt aggtaccagt
     9241 gccagttccc tttatcttgg tgacaaaata ataattcaag aaaacattgg tggtactgaa
     9301 ttcaccaccc aaggaactgt taactcaatt aacagcagtt ccggagccgt cactgtctct
     9361 tcttgggatg ccggttcatc ttttccatca ggtggattca ccgctaacgc taccgttttc
     9421 aaatggcaac gagaatactt tcctatcact tcatcgatca gcaccgacag aaacgcttcc
     9481 aatctattta ctctcttcct caataatggt catgaaggca gaaagatctg gatagatgat
     9541 attcgttccg ccggatctta tctaaccaat aacattggct ctactgttac ctcagctaca
     9601 ggcaaacgtt atttccagta tcgtgctatt tttaccagtc atgactacac cgtctcttca
     9661 tctctcactc aagttggaat cgactatacc atgaatcaac caccaaatac tcctgttcta
     9721 attagtccta cgactgcttc tgccaaccag tcacttactc ccacactcaa aacagtcgcc
     9781 actgattata ataatgacgc acttcagtat gagatcaaga tgtgtaccaa tcgtagtatg
     9841 accaatagtt gtatcacatt tgatcaaacg gtttccaaca tcggctggag tggcatgaat
     9901 gttggaacat ctgcttattc ttctggaacg actgccacct acatcattcc tgtcggatct
     9961 tcacttgctt acaacaccac ctatttctgg aaaaccagag ccattgaccc tttaggtagc
    10021 gacacctgga gtaataccca gacagacgcc ttctactttt ctaccgacac tactcccaaa
    10081 caaccaacct catgtattat cgaccagacc acctatcctg gttcttacgt attcaaatgg
    10141 acagacactt cttccgatga atcaggtttt gaagtctacc gtgtaaaaaa tggcagtagt
    10201 actctcctca ccaccaccgc ccccgatgcc agttcctata ctgacaatga tgtccaggca
    10261 aacaatacct atgcctaccg tgttcgcagc aagaaaaccg cggtcggagg taccatcgcc
    10321 tattccgact attgcgatac ttcccaactc tcaccaaaca ccggcaacac cctgttcgaa
    10381 ggactccgcc ttgaaggcct acagctcgaa taacaccaaa tcaattgaca attgcaaact
    10441 actaattgat aactcgtaac tgataactga taattaaata tggacctgac acagtcacaa
    10501 ttagaacagc taaaatcact cggaataagt atatccaaag accacctaaa tacatcagaa
    10561 tctacacaaa cagtatcaac acccccatcc ttaggcaaaa ataaacttca aactatatca
    10621 agcagttcaa cgggctcact gctaaagcca tcaattaatc ccgtaatccc cctattatcg
    10681 atctctggcc taactctact ttccttcggt ggactagtcc tttttaaagg caaggaaacc
    10741 accactctat ctcccgattt atcgggacgg tcgacccaaa gggtcgataa tgtcccgacc
    10801 caagtcccta aatccattca acactatctt cttacctctc aacaattctt taccaaagct
    10861 ctctcctctt ctccagaaga acaagtcgaa ctggttaatc aatccatctt agccgctact
    10921 caagccacca aagactttcc tcaagactat cgaggctatg aacaaagagg ccgtctctac
    10981 caaagtctta tcgacaataa tcctgattta ctaaaaaatg ctattgctga tctgtcttca
    11041 gctcatcaac tcaatcccaa ttctgccgag atcacccgca ccctcgccac actattcgcc
    11101 agatctggcg acatcaacaa cactcttaac tacctctctc aaacagttaa tctcgaacca
    11161 actaaagccc agaactttta cgatcttgcc cgtcttcaaa cacaagctgg ctatgttccc
    11221 caagctcttg atacttacac tcgccttcta accatcgttg ccgatcccac tcaaaaacaa
    11281 catattaccc tagaaaaaga atctttggaa aaactagctt ctcaaaatac aaacactaaa
    11341 gctcaacctc cactcacccc gttcaccaca cctcaatctc ctgtagacaa tagtccactc
    11401 cttcaagctg ataatggcac aggactaatt atcgctgctc ctgaaactag taaagatatc
    11461 tccgtcaaaa gtcttaccga cagcaattct ctctctggtg tcggcagtct acccgccaat
    11521 acatctcaga tttctattac caatagcaac attacctctg actcccttgt ctacctcact
    11581 attaccaaag gaggcaagaa tcaatcactt caaataatta gtaaatctac tggatccttt
    11641 acggctggtc tcgactcacc tatctctgaa gacattgaat ttaagtggtg gatagtcaat
    11701 taaaaattga tttatgattt acgatatatg atttatgaat gacacaatta agtcatttac
    11761 agatctgaac acatggaaag aaagtcataa attagtttta atgatctata agttattaag
    11821 aaaatttcca aaagaagaaa actttgctct gtgcgatcaa atgagacgtg cctcaatttc
    11881 aattactagc aacattgccg aagggtttgg tagacaaaca atcaaagaaa aaatccaatt
    11941 ctattatcaa gcccaaggtt ctttaactga aataaaaaac caactaataa catcaaaaga
    12001 tttagattac ataactcaaa cagatttcct agatgttgcc aaccaggcca acatcgctca
    12061 tcaactcctc caaggtctaa tcaccaaatc caaaagtttt attcataaat cttaaatcat
    12121 acttcatact tcaaatatcg cctacggagt tggcgatatt tccggtgtct ctgttggctg
    12181 tggtgctgcc ggtgtcggta ctcccaactg atcagccggc acatcaactc tttcttcact
    12241 agtcgtcgtt ggtaaagcct gtggcgcctt caaagtttct ggcttagctg cctcagccgc
    12301 tgctgcagct gccgcttcat tattctttgc aatcgctgcg tccaactctt tcttccattc
    12361 agtcaagtct ttgcttgccg cttcaaaatc acctgaagtc actggcacca aagccactgc
    12421 ttgttgcatt ctttggacag catcccctaa acgattcatc ttcttggcag aatatgccca
    12481 gttataccaa ccattagcaa agtctggttt tgctgacact acttgttcaa acactctatc
    12541 tgcttcatca tacctttccg ctgcatacaa caaaccaccc aactctaatt tgattactgg
    12601 attagatgga tcaaatactg ccgcctgttg ataagcttga aagctccaat cagccgctcc
    12661 atcaaccacc cccactaaat ctctataaat ggctgccaaa ttagaccaat acatcggatt
    12721 attttcatcc aaagctattg ccgattttgc ttctctaact gactgttgaa tcagagtcga
    12781 tgctttttct ttatcctcat cactcaagtt ttcattcacc aacaatgttt ttgccaaagc
    12841 cagatttgtt tgagagtaaa ctcttcgata ttctgccaaa gttggattca aaccgattgc
    12901 tttgatctgt aaattataag ttccaccacc atcatttttc cctgccgcca acaatgattg
    12961 tctcattgtt atatcaccgg ccaatattcg ataagaccaa tatccaccgt aaactactcc
    13021 caccaacaat aaaccaacca tcacccacgg ggccacatta aaaccattat cccctacttt
    13081 taatgtcaaa ccaaatctct ttccttcgcc agacaacatc atcattgtcc acgccaaaag
    13141 catcaaacca accaagttaa caggcaaaaa gagagcgacg acacccatca tcaaaatcac
    13201 tccaaaatca aagttctttt ttaactttaa tacccaggaa atcaccatac ccaagataac
    13261 catcgctact gcccccaact ctgtccacaa attaagtatc cccgaattag aatgcagaaa
    13321 accattagcc caagttgaag ttaagttata gctagctggt ctataaaaat taaaagcctc
    13381 aataaagtta cccggaccaa ctccccagat aggactcctc ttaaaagttt ctaccgctac
    13441 cgcccacgca gatgtcatat ccaagttaac ccagccagtt ttaactattc taaaaatacc
    13501 caacatcaaa gctaaagtta atacagccat cattatcgct tctattacat aaccactgtc
    13561 ttcactcttc agtttatttg ccaatctttt accccaagta accaccaaaa aagctaacaa
    13621 aatcacctcc gacaacagtg aacccgtcaa agaccaggtt tctccgatcg acaccaaagg
    13681 ttcacccgtt ggccaagtta tcggcaatct gcctacaggt attaaaaata ctactagaga
    13741 agcaattgtt attaccactc cagccccagt caaccaattc aactgctttc gtctctcttc
    13801 cttgtcgtta acctgcaacc atagaaagct ccatattaaa acagccatca ttgctcccat
    13861 ccccacaaaa tcagtcaacg acctcattct caccccttct ggcaatctat accaaccaac
    13921 ccatgcccac accaccaata gcaacaacat tccaaaagct ttattaaatt tcaaaccatt
    13981 ctctttctta ataatcattt ccaatgacca catcaacaaa ccgaccaatc ccaatcccaa
    14041 caccaacatc gtcttaccca ttccaaatga atcaattact accggcaaaa acaacagtgg
    14101 gaacaaaaac atgaatgcgc gaacataata tctagttata ctcatacaaa cagactacca
    14161 aaaaccgagt ctcccgcgca accgcgactt gtgtttattt tccgcataat tgtcttatct
    14221 tagtaccgac aacggatcca gcttcccccc agccaccttt atcacctcaa aatgcaaatg
    14281 cgttcctgtc gatcttccag ttgaccccat tattcccaac ttctgacctt gagacactct
    14341 gtcacccgcc ttaaccacca tcgatttatt caacatatga gcataaagag tttggtaacc
    14401 attaccatgg tcaatcacta cataatttcc ataaccacca gcattccagc ccgcagttat
    14461 caccgtaccc ccctgagccg ccacaattgt cgggttatct cgactagcaa tgtccacagc
    14521 ctgatggtac caataatatc tttgagagat atatcccgat gttggccaca taaagttccc
    14581 ctcccctttt actcctggta ttggtgccac tgttcttgcc actacccgag gcgcctcagc
    14641 tacttcactt ggcttcactc cgtcaggcac gataatttct tgaccagcca ccaaagcaaa
    14701 agtctcatca ttagaaaaag tattaaaagg ataatcaatc attgtctgag cattaacctg
    14761 caatttttta gcaatactat aaattgtctc tccacgagaa accttgtatc ttacccctgt
    14821 taccggcaaa attcttaata tttgttttgg cttaatcgaa ctcaccgatt tgatattgtt
    14881 ttcccaaact atagtatcta tactcacccc aaacttttgc gcgattgacg atacagtatc
    14941 gccatcttca actcgatact cggtcacacc tccctttgga aggtcagata tcaaagtttg
    15001 agccccgacc acactgtcct gagccataat taaatatccg cttccaccag tctccccact
    15061 tccagcttta tcaatcaaat cctcgactgt tcccgacaaa attatcgctc caaaaaaaag
    15121 aaaagccatc gagaaattaa caaaactctg agaaaaccta cctctctgtc tatacatcag
    15181 tcttgctaag acttttttaa tctcatcaga ccagcctagt agcatctttc ctacccgcgc
    15241 ccaatacccc gcctgtaatc ccccaaattt tcgtatcagt tcaatttcct gtttaataaa
    15301 atctcccatc agcctctata ttctatctaa aatcagctta aattactaat tttagcttca
    15361 aattaatatt ggttttcctc aaaacaaaca tgacattaca acctaattta acccatccag
    15421 tgttaattaa atccaatgta aataaatttg cacactaaca caaactactt catcaaaaga
    15481 cacgtgctgt gctttcttaa aacaaatcac ccttatttct tctctccaga tttcgtgtag
    15541 acatcttcct ttagtccatc cataaactgt cttctcttaa acatcgtttc cggttgatta
    15601 ccttctcgat ttggtacaaa gccactatca atttctaaag caaacatatt tcctgcctcc
    15661 cgcaatagat cattaatttt tgtatcgact gcacttgccc caccaagtgt cacttcacga
    15721 gacctttggc ctcttaattc tctaatttgt tgtgtaattt tagacgtatc taccatattc
    15781 atatattaac atataatcca aaatatgaac attatttctt ccctatttta gcaagaaact
    15841 cctcattagt ttttgtcttt cgcatttgtt caataactac ctctccggta tcttctctat
    15901 ctcccaaagc atcaaacatt cggcgaatag caattaccct ctccataact tttgcctcaa
    15961 gtaagagatc ctctcttcga gttcctgatt ttaagatatc aaaagctggg aaaattcttc
    16021 tttctgctaa tctacgttct agacgtaatt ccatgtttcc tgttccctta aactcttcaa
    16081 aaacaagatc atccatcttc gaacccgtat ctactaaagc cgttcccatt attgtcaacg
    16141 aaccaccatc ttcaatttta cgagctgctc caaaaaactt ctttgaagga tacaaagctg
    16201 ctgggtcaaa tcctcccgac aatgttctcc ctgaagttgg gatagccaaa ttgtatgctc
    16261 tcgccaaacg agtaattgaa tcaaaaatca tgaacacatc tttacccatt tccaccaatc
    16321 tcttggctct ctcaatggca atttccgccg cccgtgtttg atcttctggt ttctgatcaa
    16381 aagagcttgc taccacttca cccttaataa atctttgcat gtcagtcacc tcttccggtc
    16441 gctcaccgac taacactgcc attaaatgca cttctggata attcgctgcc actccggctg
    16501 caatttcttt taataaagtt gtcttacccg cttttggtgg agacactatc attcctctct
    16561 gaccaaaacc aacaggtgaa aataaatcaa ttattctagt tgataacaca tctgggtcaa
    16621 actcaagttt agcctgttta actgggaaaa ttggtacaag atctttaaac tcaggtcgac
    16681 gaacggccga cgccaattct cccccattaa ccttgtcaat tctcaccatc gaccaatgtt
    16741 tatcattatt accaggcgct ctggccactc cagtaatcat atctccaggt cggaggctat
    16801 aacgtcttat ttgcatttgg gccaaaaagg catctttggg actaggggta aattttggtc
    16861 ggacaaaacc ttgcccatct ggcattacat ccaatgctcc agttaccatt tccattttta
    16921 cgtctgattg ttgtgtttgg ggttgcagtg gcggagtttg aatccgcggt gcttgatatg
    16981 ctggggctat cggttttact ggagcaatta ccactctatt attacttttt ggttcttctt
    17041 tttttacctc ttctaccact ggttgcgctt caaccttcac taactcgaca ggactttcga
    17101 cttttagact ttcagacttt tgactttcgt cagttttttt ctttaccata aaatctctct
    17161 aaaaatataa ataaaattac taatttgttg gggaaaatct tttgatttca atttcgtgaa
    17221 aatacgctaa attacatcag aaacgtgcaa ctctcaaggg gaacaagact attgtaacta
    17281 atatattttt cattgtcaag tttatttccc caaatacgct ttcttccata tcgtaaactc
    17341 caccaagcta aactcaccct ttgcatattt acccttccat actccaaaat caaccaaatt
    17401 aacaacctta tctccattaa aatcccctgg tatgtttgtc actggcaaga tattttccct
    17461 cttacactgc tctaaatccg aaaaacactt gccggtcgaa tcaggcatat atttggtcaa
    17521 gttactctca caggacactt tttctgtttt attacattca aaaccgtctg agttgtatgt
    17581 cgacgatgtt attaagcact gattactcac tttattaaaa taatattttc tctcacaagc
    17641 ttgccccaac ccattatctt tttgacattg atcaaagctt gcaaaacata ctccggtaga
    17701 attgggtaca tattttgtca aattactttc acaggttacc ttgttttcat tattacactc
    17761 aaaaccatca gagttatatg ttgatgaggt tgttgaacat tgtttttctc ccttaatata
    17821 gaaaaatttg gtactacaag tatttgtgta atgcttcaga taatcctctc gtaatttctt
    17881 tcttgccact gctttttcca gcgtgtattg cttgctccag ctattaaaag tatcaaaagt
    17941 cggatctgaa agcatcgtgt gttggtagat ccatgtaatc atttttgaga catatggctt
    18001 tgccgtatct acttgagaaa caatacgact gatcaccgcc ggattatagt tccaatctcc
    18061 agcaggagcc tcaaatgttt ctatgttggc ccaagcttct ttatttggcg ctgataactt
    18121 cgcatctttt aaagcccgaa aatgatccaa attactcgca cttgttgtta ccttgccact
    18181 cccaatacta tcttgcaaag caataatatc tatcgcagta gacttataga cattttgata
    18241 ctcattttta agttgatcat aacttgtagt gttataaaca taaggtgaaa tcagaatttt
    18301 ttttgttggc gcaagctgtt ttattttttc agacaacttt ttataataaa taaaagttgg
    18361 cgaatctata ttactaaaca tgcctggcca agcttcttga ccaatataat atccaacgac
    18421 aagatcatta ggtaacccct gtcgaacaag ttctttttct gtagcttcaa ccaaacgccc
    18481 agaaaaatct atcaaacgac cctgattggt atttatatca gttggagacc cgtcactaat
    18541 atttatctga ctcggatcag cacttgccaa tccaacaaac aatttcatat tcaactcgtg
    18601 agccaaggac aatatatctc gactaaaatt gtagtcaggg ttattgtaat aaatcatctc
    18661 actaaatatg ttattcgttt tcgacaagtc ccctgaagcc aacatgataa cagtatcaat
    18721 accactagcc ttcatctctg tcatgatctg acgaatttgt gctttagttg ccacaatatc
    18781 cgacatataa aaagtacccg taatcggcaa acttgatgct gcctccactc tcactacacc
    18841 aaaactgaaa aacatacata tcaatatagc tgctactttc atctatctaa attaaaacac
    18901 atccctctcc tgttttctac cgctgccaca ccaaagtcaa acaagatatt ttttcccgta
    18961 aaaccggcac caaaaatttt gcatttttca tgcatttaat atatcttaga tcttgaagaa
    19021 acaattatat acgtgttcca cctgctttga gaatatcgat atgattgttt ctttttgttt
    19081 ttttgcgtaa attttcaagc tataagcgat gaaaatttgc tcaaaaacac aacgcaattt
    19141 actaattgcg taagttcatt tttttctaaa gtactaactt ccctcccaac tttgccatct
    19201 cctgtatctg ttttcgcgcc caattatctt tcttctcctt cttaaaccaa ctcttcaccc
    19261 atgcttggat tttatttagc ataattaact ataactcttt tccagcactc gtcaataccc
    19321 tatattattt tacctcccct tttttcataa tcctatatat tttcttttca tacatttcag
    19381 gttcaaaaac acccacttac aaaccttttc aacatcatgt ggataactta gtttgacttt
    19441 accccagttc agagttatca tccaggcaat tacaattcaa tctaactatg tgtcaaaatg
    19501 gtacctgtaa agcctgtcga caaaaacaag aaaggtacga ggaattagat gcaagctcag
    19561 ataatagagc ccaatacctc atccgtgaac taacagaact aggcactcca gaagcattgt
    19621 caagagccga aaaacagaag gcagggatag ctcctgaaac cagtcttacc gtaagtcttc
    19681 ccaaacaatc caagagataa aatacatctt aattttggtt atcttcccca ctcctctgct
    19741 cccaacattt tcgcctctag ctcatcataa ggtgcgataa acactaatac catcccatcc
    19801 ggatctttga ctaccatctc cagtgtcccc caataataat gtctgactcc tactgccata
    19861 tcaatatgtg ctttattaca aacttctata acattactta atttatctac gttaatcatc
    19921 agagatatct ttgaattcct cactgtttcc ttaaaaacat ctggcttcac agcctggtgt
    19981 ccattagcta tctctaattt cattcctcca acttcaaata ttgtcccatt ataattttcc
    20041 tttacttccc tatctggccc aaaagcaaat acttccttaa agccaaaagc tctataaaac
    20101 gcctctgact tggtaaaatc ttttactttt atatgtactc ctccacctgt atattgcata
    20161 ttcaatttaa taaataatca gtattgcgaa gtagccattt tttggagcac agcttctgga
    20221 gccatttatc ggcgaagcac gcgagcacca agaaaatggt tacgagcaat acatagatta
    20281 tttgcataat atcaacttta ttgacattta ccaaatattc tatatacata tatacatatg
    20341 aagatatttt ttacctgctc ctacactggc aaagccaagt accaaaaata ttatgatttg
    20401 atcatagatt ccatcaaaaa aaccggcgtc tccctcagct cccctgaaat cgacgtttcc
    20461 ttccccaccc ccgacgaata tccccattat caaaaaattc gtgatggtat tttcaactcc
    20521 gacgccgtca tcatggatat tactgatgag ggtttccaac ttgggtatga ggcagccact
    20581 gccatccaaa acaaaaagca tgtattagtc ctatcccttc tacgtgactt tagtcaaaaa
    20641 gtcgacaacc gctattttca tgccgccaaa tattctgaaa tgaacatcga agagatcatt
    20701 agtgatttta ttaacagcct ccgtcgccat gaatacgatc aacgcttcaa ttttttccta
    20761 tccaaaaaac agctcgacac cctaggtcta aatgccacca aatcaggtca aaccaaatca
    20821 gactacctcc gttctcttat cgacaattcc taacttttag aatttaggat ttagatctta
    20881 gaatttatat caagttcctc aactctctta tactatttat acataccatc tcccgtttca
    20941 ttgccttaac ctctttttct cgtttttcca ttcccctgac tttcttcacc tctcccaata
    21001 aactcacctc tcctactagt acgggcggac cggtgtgtcc gccctttcct gaaattattt
    21061 tttctttata gctactaaaa attgctgcca tgattgctag atcacaccca gtatcatcta
    21121 atcttatccc tcccgtaact gatatataaa catcatattt ataaagtggc atccccagaa
    21181 ctttctgggc cactgccacc aaaagtctcc ccctatttgt atctattccc gaaaatactc
    21241 gagttggcat cggtgcaaaa ctctcagtta ccaaagcttg aatttctacc atcattggtc
    21301 tagttccttc cattactacg gtaatagccg aaccaacttt ttcttgattt cccgaaatca
    21361 aatttatctc atcacttttt atttctcgaa gccccttctc ttccatctta aatactccca
    21421 cttcatccgt cggtccaaat cggtttttcg acacccgcaa aattcttaga tccccattct
    21481 tctctccttc aaaataaagt actgtgtcta ccatatgctc caaaagtttc ggtccggcaa
    21541 tatccccctc cttagtcaca tgaccaacaa taaaaatagc cgtcccagtt ttctttgcca
    21601 attccaccaa tcgaaaagtt gactctctaa tttggttaac cgacccgaaa gatcccaaag
    21661 tccctgtccc cgttaacgtt gacccgacca tcatcgtctg aatactatcc accacgacta
    21721 ttttgtacac attatttgtt cgttccaata tttctccaat ttcttcaatt gagttagtct
    21781 ctaaaacatc gaatccttcc gcatttaacc ccaatctatt cactctaagg ttaatttgtt
    21841 ctgccgattc ctcaccagcc acatacaaac caccattttt acccaccaat tgggttaaaa
    21901 gagtactttt acctataccc ggttctcccg caaatagcac caccgaccca cgaacaattc
    21961 ccttacccaa tactctatca aactccgata tattagaagg aaatacacta tcaatttttt
    22021 cttttctttc gaccaactta ctcagattct taacttcaga tctttctctt ttctgtttcc
    22081 cgctcgtaac tcgtaactga gaactcttaa cttctttaag tgaattccac tccccacatg
    22141 ccgaacattg ccccgaccat tttccaaatt cattcccaca actactacat acaaaaatac
    22201 tttctgactg tttcttcata ctccatttta ccattcgtgc gttttacgag ctctagtgaa
    22261 gtcatcactt ttttttgcct gctttacgca caaatactta cggacgccac tttatctgac
    22321 tttttctaat ataagtccct cgataataaa cccactgatc cctccaatca gtcagtggag
    22381 taacttttgg tcgactcgac aattcaggtt ccggtttaac tacagggctc ttaataatct
    22441 cccgaccttt ttctactgcc attcccgatt atatcaccaa aaactctcca ttacttaatt
    22501 atttttcttg cttccaagta aaacctcttc tcatt
//
LOCUS       DDWP01000054            7046 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_22164, whole genome shotgun sequence.
ACCESSION   DDWP01000054 DDWP01000000
VERSION     DDWP01000054.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_22164"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    279..292
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2292 a         1730 c         1047 g         1963 t
ORIGIN      
        1 tcgtcaaaaa ccaacgactt caacgatacc tcctgtccag gaaacctagc atttttaccc
       61 caaatacagg ctctagagac atcgtctctc atttcactac taatcctttt caatacatca
      121 gacaaactat cactcttctt cataatcaat ggctcttttc tatcagcctc cgccactcgt
      181 tctcttttta aatacaccct caccatcccc aaacctctcc acaccgcttc tttaaattct
      241 tccataccca agccgctttt agcactcata tataccacnn nnnnnnnnnn nnggaatata
      301 tgtcctcgac ttactcaaac catcaataaa ctgatcaaga ctctcgattt tttcacccaa
      361 ctgtatcact gcattttcca tccccatctc tttagcaatt tcagccattg tatctgtcgg
      421 taaagaaaaa aacgggtcaa taatctgaat tgccccctta cccatcttat ggatatgaat
      481 tttgggaggt ctcttattta atctcatgcc agcactctcc aactcttgtt tcacctttat
      541 cagccaatcc gctctcttat agtcagtcat caacaccacc aggttcgacg ctctaattac
      601 ggacaaaacc tttcttccaa aacccttgtt cttggctgca ccctcaatta tacccggaag
      661 atcaagtatt tgtatcttaa ctcctttata ctccatcatt cctgggacta cccctagagt
      721 tgtaaagtcg tagttaccca ccttcgattc agcatttgtg atcgcattta acaaagtaga
      781 ctttcccact gacggcaacc ctaccaagac caccgaagca tctccagaat gttttatggc
      841 ataaccaaca cctccacctc cacctccact aattttttgc tccaacatgt cacgaagctt
      901 ggaaagtttc gcctttaaaa ctccatggtg aaactgacta cttttatcat atggcgtatc
      961 gcggagctct ttctccaccg ccaatatttt tctttggatt tcttctggaa ccatttgtat
     1021 agtaaaatga gactcacggt attatatgaa aaaaacacta ttttttctct tcttatttat
     1081 attttgcgga ttaagtttta cgtcttacgt ctttgccgaa agcaaaaaca tttttggtct
     1141 ccaccttacc cagacccaag atattgaatc agcctccaaa gtcatcaatt ccggaggagg
     1201 agactggggt tgggctaccc tagttatcag aacagatcaa ctagacaaaa atgtttggca
     1261 agatttcttc aataagtgtc gcgaacttca tattataccc atcattcgta ttgccgccac
     1321 catggagcaa gatcattgga aaagaccatc catctctgac atagattcag tctccgagtt
     1381 tcttaattct ctaacctggc cgacagccag acaacacata attttgttta acgagatcaa
     1441 ccatggttca gaatggggag gatctgttga tataaaaagt tttactgata tttcgatata
     1501 tagttacaat aaattcaaat cccttaacaa aaacttctac atactcagca ctggtctcga
     1561 tctagctgct cccgaaaaac ctgaccaatt caaatctgcc gccaacgtct atcgtgaaat
     1621 atatgtctat agaccagaat attttgatca ttttgacgcc ttggcttcac actcttaccc
     1681 caaccacggt tatataggta cacccaacga aactggccaa cacagcatcc gcggttatca
     1741 atgggaacta aattttataa aatcattagg tatccagaaa acatatcccg tttttatcac
     1801 cgaaactgga tggcctcacc gagaagggga gaaggtcaac aataactact acaccgccga
     1861 gacagcctct aaatttctta tcactgcttt agacatctgg caaaaagatc cgagaattat
     1921 agccgttacc ccatttattt acaattaccc gtacgaaccc tttgaccatt tttcgtggct
     1981 caacaaagaa gagaaacttt accccagtta tcaaaaaata ctctctttac caaaaacaaa
     2041 aaactcccca ccgcaaacta ccatttacga aattgtgaga aacgaacttc ctttccttct
     2101 tttcagtgat atcgagtatc ccggtaagat aactctcaag aacgccggac aatccatctg
     2161 gggtgagaaa acgatctgtc ttacacccca atctacagcc aacgtcacac tagacgctat
     2221 ttgcaccagt gatcaactta ccctccccgg acgtaaccag accttcaatt acaaaataaa
     2281 aataaattca tctacagatc accaagataa aacattcatc agttgggaaa acatagcccc
     2341 attagaaata gtctccatta acgattctgg aagtatctac agtccaaaaa caacttttta
     2401 tcaaaagaca attcaattct tcaataattt atttaagtaa gtgtgccgct ggagggaatc
     2461 gaacccccac agatttctct atacgctcct aaagcgtacg tgtataccaa tttcaccaca
     2521 gcggcctatg agtagcatat tttatcaaca attttgataa aatagcgcta gttgtcctcg
     2581 tagctcagtt ggatagagcg cttcccttct aaggaagagg tcacaggttc gatccctgtc
     2641 gaggatactc aaaagaaaag cgcctatagc tcaatggata gagtaccagt cttcggaact
     2701 ggtgatgtgg gttcgattcc ccctgggcgt acttatgcga tataattcct aagttaagtc
     2761 acttgggttg ctagctcagt ggtagagcaa tcgcctctta agcgattggt cgagagttcg
     2821 atcctctcgc aacccacctt ttttctgcta cactatttta tgtggttttt tgttttattt
     2881 cttcttttta ctactcctgc tctagctcag gatatcaccc ccagttcagc ccctcaagag
     2941 acacccacaa caattccctc gccaacacct gcccctaatt tctttgaaca atacaagaaa
     3001 gattatctct tccaatacga tatctatcaa caatcatatc taaaatatgt agacaagaaa
     3061 cgtgtttgga ctagatacgg gactatcact acccaaaaag aaaagtttga tgcttcaatt
     3121 gaagccatta ctgctcgcaa taaagccttc aaagcctacc ttctcgcttt acgaaccatg
     3181 cttgacgact acgaaagcgt taaccccacc aatactgaaa aatatcagat agaaattagt
     3241 aagtgggaat catggttcga agaacagcta tctgtcgttc cttccatcaa caatgagaaa
     3301 gaccttatcc gatgggttga tgatttcaaa gaaaaataca ttgtcattca acaaaccatc
     3361 tactcatctt tagttcaaca tgaagtcaac ctaagacaac taactttaga aaatatccag
     3421 tctctcggca tggacattaa gaccaatccc caaattcaaa cagaaagtca gcaatgggtc
     3481 agtaccctag ctatcaaatc cgatctggtc accaccagtc tcaacaatgc catcgccttt
     3541 ggtcaaagaa gtcaaagtca aaataaattc aacaacttct accccagctc taaactggaa
     3601 ttaaaccagg ccaacaacta tcttatcgaa atctctgcag atcttaaatt gatagttaat
     3661 aaatttttca agccgtaagt atggacaacc cttatcaaaa tccagattta gaagtcagaa
     3721 acccttttgc cgattcggtt gtgccagagc ctcaatctcc taacactccc acagcagcca
     3781 aacccaacaa taaaaacttc tccatcccca aagatccaaa aataaaaatt ctaattatct
     3841 tggttgttat tattgctgtg ctctcagtac ttagctttat tatcaccatt tttcgtaaat
     3901 cacagctaaa accagtcata gtcgagccaa caccgataat agaaaccact ccagtcccga
     3961 ctgaaacacc cgccctcacc atgccggcag aaatcaatgc aaaatttgaa aagatagatc
     4021 aaaataccag gaccaatatt cagtttgacc ccccacaaat agatccagat atcggactat
     4081 aacctaattt tttctcgaca aatattgtcc agtttcagta ctaattgaca cctcttcacc
     4141 cacttttata aacaaaggaa ctaaaacttt agccccagtt tccaatacag catttttcaa
     4201 agcgttagta gcagtgtcac ccttaacaga attttctgac tcaactactt ttaagataat
     4261 actctttggc aaagtcaaac caactggttt ttcttcgtag aattttattc tatagagtat
     4321 tccttctttt agataaattt tatcttcagc tactatatcc tcagccaccg aatattgttc
     4381 ataagttctt ggttcgataa atacgtagtt gctaccatca aaataagaat attccgcttc
     4441 atgagtttct accaacacct cttcaatttt ttccccggat ttataggtat attcgaccac
     4501 tctaccagta tataaagatt tttgtttaac tcggtaaaaa gctgaacctt tacccggtga
     4561 gacgaaagta atatccacca ccatgtgtgg ttcatcgtga aatattacgt acattccgcg
     4621 tttcatttgt gctgttgata tcattcccat aaggctattt taactctcca atctaaaaaa
     4681 tgtaaccccg tcaatatttc ctctcttact aagagataag gttaaaaggg gtgatttaga
     4741 ccaaaaaaat cccagagtct gctttttaga ggagccaccc cgggatctag ggtgatttat
     4801 aacaaaatat ataagctaag tcaaggtaca aaatagatat gtatctagca atcaatgtat
     4861 caaaaatccc cagactcaat atcgttaatg aattgtctca ccccaaaagc ccaagaacca
     4921 ttagaacttg gacaatactt cttcataatc agctccggag tatctaatcc tttatcaaaa
     4981 taaacccttt ttaagtactc cgcataactt tttaaagctg tttcgtaatt ttcaaatctt
     5041 agagtccctt ttgaatgtat cccataaccc caacaattaa aagaattctc aggtatcttt
     5101 ttacacagat ttgattcctg ctgacctata gctacgatcc agtaataagg gaatccatat
     5161 gtttccgaca attcaaatat aagatcagag taggggacta atggcgaatt atatttttcc
     5221 aagtactttc taatcaacaa aggcctagca tcctcactct caacccgact agaagcctca
     5281 ctcacacccg tcaagccttc ttctaccccg ttagatgccg ctaacactac actgtcgact
     5341 tcaaccttgc caaatagcgg ataactccca gtctctccat tgtatttaaa actcaatgca
     5401 cccacaatca aagtaactac cccgagcatc agtatggttc cagagaaaat tatccttcct
     5461 gcgatagcga ttgtcttttg tgtctcgttc acgtatatcc atgatatcat attcctactt
     5521 atgactacca aaaataatgc tccaaaaatc gtcaccaaac ctgacaaaac cttcactatc
     5581 aacctaaccc tcactaccga ggaaatcaat tccggttacc aaagcaccct caaatctatt
     5641 cagtcagatt ttgagatcaa aggctttcgc aaaggtaaag ctcccttaga tgttgtcaaa
     5701 caaaatatct ccgaatctaa ggttattgaa gaagtcctca gccatcttat ctcccatgcc
     5761 tatagccaca ttattaccga caacaagctc aaaccaatca tccagccaca ggtaaaaatt
     5821 ctcaatccac ccattactct cgacaaagaa tggcaagttg aaatcactgg ctgtgaatta
     5881 cccgagacca ccctcaatcc aaaatacaaa gatgaggtca aaaaaatcaa cctcagcaaa
     5941 gatgacgaca accagaaact aaccaatata atggaatcta tggttaaaaa tagtaaagta
     6001 gacatgccat ctattctgat taaagctgat gtcgagaaaa aactagccga cctagtcgac
     6061 caaacccaac aagccggact aacagtcgct caatacctta aaaatcgcca acaaacagtt
     6121 gaacaatatc aagccagttt agaggttcaa gttaagaatg aatggattac caatctagcc
     6181 atcgacaaga tcgccaaaga agagaagctt gaggtcagcc aacaagaagt tgacgagctg
     6241 gtaaacaaaa acaagcaatt ggcccaaaat ctcaaccttg tctattacct cctaactcaa
     6301 caaaaagtct tcgaattttt gaaaaaactc tagtatctag ataacgtctt ataaaaatgc
     6361 tacaattccg ccatgtccgg caggttcaaa gggtcattta accctaaaaa tcctaaagca
     6421 aataaggcat tcaacagcga agttttaaag gaagactccc aaatcgacaa tgtcgtcaaa
     6481 aaaccggcca cttttagtct caagggcatt cttggcctcg gccaatcagt agagatcaac
     6541 aaacccgtca cttcccacga atcttttttt ggtctaaatc atttagccca agagcaaaat
     6601 atcctctttg atcaaaggca gcgagaacta aaacaagcta tcgaagatct tcggctagaa
     6661 atcaaaaaat tagcccaatc tacctcaaat ataagcacag atgtcattcg tatagccgat
     6721 tctaatgtaa ttgatgcaaa tagctatcaa ataagtgttc ttactcgtat caaaaacttt
     6781 attattgaca tgcgcaaaaa cattaatgaa gccagcctgt ggatggaagc atttgctagc
     6841 aagaaaaaga agaaaaatct attttggaac aaagttaaaa acaagaaaca aggtggcgat
     6901 cagtatttgt tcagcaacga acattccgcc gccagaagtg taaattaatt tgttataatc
     6961 cctctattaa gagtctatag ctcagttggt tagagcgcat cattgacatt gatgaggtca
     7021 gaggttcgag tcctcttaga ctcaca
//
LOCUS       DDWP01000055            7683 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_26097, whole genome shotgun sequence.
ACCESSION   DDWP01000055 DDWP01000000
VERSION     DDWP01000055.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_26097"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2743 a         1109 c         1772 g         2059 t
ORIGIN      
        1 actgagaatg atcaattttt tagatttgag gaaggtgagg gaaaggtaat catagacgaa
       61 aatgagtact tggtttccga tgggacagca gtagtggtac cagctggatc aagacacaat
      121 gtcattaata cgtcgagtac tcagaaatta aaactgtaca cgctttactc ccctcctcat
      181 cacaaagaca aaattgtgag agcaacaaaa gaagaggctg aggccaacga ggctgatttt
      241 gatggggtaa cgaccgagta aaaaactcat atccgataat attctaataa acaacttatt
      301 tttattgagt tttttgatgc cttggtgtaa aataccatat gtctacggaa aaagagctag
      361 gcaatagaaa gtttgatgat ataagagata tttccccgct tgtaagaaga gcttataact
      421 tagcagaaaa ggcacataaa ggtcaactaa gaaggtcagg agagccttac tttactcact
      481 gtgtagcagt ggccaacatc atatatcacg aatggggaat agaagatgaa aaatatgtgg
      541 ccgccgcatt gctacacgac gttattgaag acactgaaac cacaaaagaa gagattaaga
      601 gagattttgg ggaagatgtg cttgagctag tggagggagt aactaagtta agtcagggaa
      661 cagatcaaga gacattgtca aaggtaattg acaaaagcta tcttaaccca ggagtggcag
      721 ttgtgaaatt agcagacaga cttcacaaca tgagaacact tgggccaatg aaaccggaga
      781 aacagattga aaaagcccag gaaacaatga aagtatatac ccgattagct gagtctttgg
      841 gaatgtggca agtaaagaga gaattggaag atttgtcatt tatgtatctg gacccagaag
      901 gttatcgccg aataaaagag caaattgata acgatccaag aaacaatccc ctgtttatag
      961 cttatgtaaa aagtagccta gagcaattga tggccagcaa tggatttgtc ggtaaagttg
     1021 aaataaccaa aaaaggttat tgtgagtcaa aaaagaaaca agagaaaaaa gctatgaaag
     1081 ccgaatgtcg accgggaagt tttgaaggag taaaggatgt cgttagcttc agggtaatac
     1141 ttaatgacac tgcggactgt tatgtttttc ttcatcaaat acacgaacag gaaggaaatc
     1201 gagtagattt tggacaaact gaacaacaaa ttggtgctaa caaggcaact aacggctatc
     1261 aagctctttc cactacgatc gcatacaaac agggaatgac tgagatagcc atggtaactc
     1321 ctgaaatgga ggaatttaat aaatggggaa taataagttt aatcaaaaaa ggagagaaag
     1381 acctaagtca atattctcta aaaatagttt tcacaccaac aggtagtatc cgctttgtac
     1441 caaaagaggc aactgcggct gacttggctt tggaaattgg gccagcagta ctggctaatg
     1501 gaattggagc aagatctaat ggtgaagaaa tggaaccaat gtcgacagtg cttgagaatg
     1561 gggccgtagt agaagtgata ttggcccagg agacaagaag agccccagag ccagggctag
     1621 aagattactg ctctatgcta caaacaaggg cagccataag agaaatgaga atattagccg
     1681 agagagatga gcgaattgaa caaggtagag aaatgctgga aaagatattg tcaccacgag
     1741 gattggtgga tctgacagac attgaacaga ataagttcaa ttcagtgctt tatcaactag
     1801 gatgcgatgg atatgaggac ttaatgttta aagttggaac tgggtcgatg gatatggtga
     1861 tcttgaacaa ggcattagat caggcaggaa taacgaagag ggagctagga gttaccacta
     1921 taaagttagc tgggactgat ggtgaaggaa tattgaatat ggttgacact tggataacaa
     1981 aaaagaacaa gaatatacat caaatagcac acaagagaaa gggtagtcag tatgagctaa
     2041 gaatagttgt tgcggatcta acaagaagac aagaaaatga attgaggcgt ctattgaaaa
     2101 gagatgggcg atttgaagtc agcttggtag tctaatttgg atataatatc aactaagata
     2161 agttcctata gtttaatgga cctgcctacg cgaaacgcta cggcaaggca cagtagaatt
     2221 tttaatttgt tctcgtggct caattggata gagcgtcggc ctccgaagct gaaggttgtg
     2281 ggttcaaatc ccaccgagaa cacaacgtaa tggttcgagt atagtccggg gtataatagg
     2341 aacatggcag aacagcagat tcatgaaaac ttgtactacg acgagagatc ggcggggggt
     2401 attgtataca gaaaagaggg tgacaaagta ctttggttac taataaaaac tttggccaaa
     2461 agacagtcaa atactcgagg gcaggcaggg atgaggacaa tatataaatt tcccaagggt
     2521 catttaaaag ataacgaggt attaaaacaa gcagctttaa gagaggtaga agaagagggc
     2581 cgaataaaaa gtaagatagt caccaaggta ggatcaaacg attatattat ctgggacaaa
     2641 ttgcaaaaga gaaaaataat taaaaaagta actttctttt tgatggaata tgccgagcaa
     2701 agtaatctaa aatattatga tagtgaagta gtgttggaga gagaatggtt tgaatttgat
     2761 gaagcggcac ataagctggc ttttgattcg gaaaaggtat tactcaaaaa agccaagata
     2821 aagctggatg aattgttaag agaaactcta aaatctaaac actaaattct aaaagattgg
     2881 attattggaa tttgtaggta taaatgagat aatgagaacg tcttagaata tggaggagcc
     2941 cgcctacgtc tttcgactac ggcagggtaa atcggattag tggtttcccc tttcggggac
     3001 atccgcttcg ctcctgcatt ccaccctaca cagtctgggg atagaataga ctgaatatgg
     3061 aggagtcgga tagtggttca ttccacagac ctggaaagtc tgcgctccac aagggctcgc
     3121 gagttcgaat ctcgcctcct ccgccaagaa atggcattac caactattaa tgtagatata
     3181 aaaagtgaaa ctgtaagcaa ggcaattgga ttgattgcta aggctggata tgtactgtac
     3241 gaaccgtaca gaatcaaaaa gaaggccaca gccgaatctg aagctaaaat aattagcaca
     3301 aaaaccgaga taagaatttc atcactgcaa cgacaagctt tgaacagaat actctcgaca
     3361 gaaacattaa gacaagagaa tataaacaat ttgaccggta aagctttaaa tgaaattgtt
     3421 tctaatggaa aacaaattga taatgccact gatgtagact ggataaagaa atactttggt
     3481 ttttctcaag atatttcaaa tgacgagatg caagatatct ggtcgaagat cctgtctggt
     3541 gaattaacta aaagtggaag tttttcttat aagacaatgg cgattttagc caatatgaga
     3601 aaggaggaag cagctttatt ctccgtcgtg tgtcaatatg taataagagt aggtcaaaga
     3661 ttggtccctt taatatacga ggatgttggg gataaaattg acagcgggta taacgaaggc
     3721 actttaagtt ttgagaattt gcagttttta caatctttag gtttaattca ttttaatgag
     3781 gtgggagaat atattttatc ttctccctca aaagggattg ttgtgggttg ctccgaaaag
     3841 aaatttctaa tttcatctga agttgatggg aaaaatgttt taaaagtagg gaaagtattt
     3901 ttgacaaagg aagggcaaga attatgtaca attattaatg aaaaaagttc gttaaatttg
     3961 ccagaaataa ttaaaaacag attggttact gaaaataaaa ctatcaaaga gttatgaaga
     4021 tacaaaggtc caagactcac cttctcccct catgagaaaa aaaatatttt ttatcagaaa
     4081 ttacaagttt gatctattga ctgctttctt tgcctgtatt tcatattttg tttcgttcaa
     4141 atatgccaac aatcccaaaa taacactctt cctatctttc ttgattactt taatttctgt
     4201 tatttttatc gtcgcgttgc gattaaggga taaagatttt tattttattg aactaacaaa
     4261 agaccaagat aaaaatgatt ggattgggag gggtactttt gaatatagaa gaacaaataa
     4321 atcattttta attacggaag ctgaccctgg ttatattttt tcaaagacat tgaattggag
     4381 cgattataaa ttcgagtctg aattcaaaat gcttaataaa gggttgggtg taatcgtcag
     4441 agctgtaaat ctttctgact atataatgtt tcaaatttgt ccaaatggga tcagaccaca
     4501 tattagaatc aacggaggat ggcaaatttg ggaagcaaaa gaatctaatt tggaatttaa
     4561 aagaaaactt tcttttgata aatggtatgt gttttctata acatgtgaaa aagatattct
     4621 tgatattaag ataatggaaa aaagcaaaaa gattttgcta caaaggagct gggttattcc
     4681 ccatcaacgt tttgggtttc cttttccaca acaggagaat gatccaaaac ctctagtaat
     4741 tcactttccc ataaatcttg attatggtac tgtcggtttc agaaattcag gaaatgaaag
     4801 agcattaata aagaatgtac taattgaaaa agtttaattt ataaggatat gatagttcct
     4861 gatttatttc cccctatcag ttttgttatg atttggagtg gggggaatat ttaaaatatt
     4921 aggaaaagtt tttttgggga tggttgtact ggtagttatt tatgttgtga cactttgttt
     4981 tcctcagccg ttttttaaaa ataaagttgc ggtgggagat gtgacggtct actctgacag
     5041 ggatataccg attgaagaaa taaccaacat tgttaatgaa gcccaaagtc gagtggctaa
     5101 atctgtcctc tacaaaccgg gtatgaaaca aaatgttttt atgtcaaatg acttgtggcg
     5161 atggcggtat ttttcgaaca tccactataa ggcgagtggc ataaatcaag ttcttttcaa
     5221 ccacaatata tttttccgaa aaacagacat aaagaataac aggctctatg gccaatctgg
     5281 aaatgtggcg cctggggatc gaacttttgc ttattttctg gctcatgaaa tgacccatgc
     5341 cctagaattt caatccatgc cttggtacaa ataccccctt caaactaact gggtattgga
     5401 aggatattgt gaatatattg ctcacggctc gcaaagttac gagacggcac tgaatcaata
     5461 cctaaacgta ccggaaaact caggagagaa atattacact agggccagga ctatggtggc
     5521 gtatttatta gaaaaagaaa aaatagatat ttctcaaatt tgggcaaggg cgagtgacta
     5581 tgattctgta ctgaaagtgg ttattcctga tgacagacca agtttggcga attaataaaa
     5641 aagaattcta aacttgaggg cgtataattg agatatgttg aaccaactct cagaaataaa
     5701 taatagatgg aatctgccta aggtatattt ggaatatctt gaaaagcatg aggaggatga
     5761 attcttagaa gaaatgagat tgtatggttc tactaatttg attagtaacc aagaaggtta
     5821 ttcttataac ccagtaactc aaaaaataat agaagtgtgg ccagaaaatt atgtggtaat
     5881 tgctgatatt gatgccgatc cgttcgtact tgatttgtcc aattcagacg gaaaagatgc
     5941 accagtttta tttgcgtatc atggagaggg cgaatggtct tttgaaaaat gtgctgattc
     6001 ttttggagaa ttcatatcag aaaagcttta ccaaaacaat ttctaactgc atcagttgta
     6061 taatctgctt atgaacaaaa aagtaatcct gattggagta gttttgttaa ctattattat
     6121 cggtgcttat tattttttag gtggtaaaaa agagaatagc aatgctgaac aagatgtttt
     6181 aagtagcaca ctgtccatct cacaggaata ttcgaggcta agatatcaga ctgataatgc
     6241 actagtaaag gcaaaagatt accagagtta tgatgattgg aataaggaaa tgtcttcaat
     6301 cattgagggc tggaaaacat tggaaaatga ggtgtcggaa ttagaaaaga atgctgataa
     6361 attgactaat caaaaaacag taactaatct agtaaagggt atttatgctt atgattctgt
     6421 agaagttcag aaggtgatag aaaaagcccc gatggggaga caagtcagaa cactagctaa
     6481 acatttaggg gtagatgcca aaatggctca gctaattcta aacgaaagcc aggatagaat
     6541 atcaagagag atatggggtg gtgaaggaga tgtctaccag ggattggaac aagatgcgat
     6601 gagaattaag aatggagcga aagtaacggt gtttgtggga ggggtggtgc taactggagg
     6661 gatgtcagca gtagcaacta gtggagtggt agctaaaacg gctctagtag tatcgggggc
     6721 tgacctagta cttgaagtag ctgatgatga agctaagata gctctaggag atagaaataa
     6781 ggtgtcaaaa atggtgggta aaattagaac tgtaactgag ccagcagcca gcattctgac
     6841 agttgtgaat atgccgggaa atctgtctaa agcgatggaa aaagtaagcg ctctgtcgtt
     6901 tgctggagat cagattagat cggttgctca agatgaaaaa attttgggaa taaatattaa
     6961 ggtggatgag aagggcgagg tagaagctaa ggtgagtggg ttaacggaag aagaattgcc
     7021 aaaatggcgg gaagaaaaca atgcgactga atcaaaccaa acagctgaag agatacttgt
     7081 tgaaagaaaa gaaagtgtag tggaagaaaa aacaatagaa aaagaagaag ttgacagcat
     7141 gacaggttca ggttgggaag gaacactaag cagtatgtcg gggggcgata atcaaaaaag
     7201 aactattgat tttgatttta tattaaacca agatggcagt gtgagtgggt cttcttttaa
     7261 aaaatggaaa caaaatggag acagaattaa gatgtatggc gaagatgagt ccactggtta
     7321 ttatgagttc aaggtaagca aacacggcct cttgctaact aaaattgtta ttggagatga
     7381 agtgatacag ccaggagaag agtatatggg aggaattgct cctgcaggct atttatcaag
     7441 aaaatcaagt tcagaagaga acaatttggg atctagtaaa aatgcaatgt catttactga
     7501 atacaacgaa atggatgata aaggattact caaaaacata ttatcggtag aaaaatattt
     7561 aggagaacca gatgttaaag ctaccgacga caatggtcga gctgtttatg tttattatga
     7621 cttagttaaa tatgatagtg gaaatctagg tagcgtaaaa atgacatttt acgatgaaga
     7681 tga
//
LOCUS       DDWP01000056            4667 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_42568, whole genome shotgun sequence.
ACCESSION   DDWP01000056 DDWP01000000
VERSION     DDWP01000056.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_42568"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1326 a          973 c          879 g         1489 t
ORIGIN      
        1 acattccagt gttcaaatcg taaagcatta caccaaagac caacttaaag ataaattagt
       61 tctcggtgtt gttaactttc caccccgtca aattggtccg tttatgtctg aatctcttac
      121 cctgggtgtc cctgatgaaa atggagattg tattttagtt actccagaca aatcacagtc
      181 tattattggt ggtaagctat tctaatatga aaacacaact tgctgatttc aacactaatc
      241 tttctgaatg gtttaacgat atagttctca aagccgaatt ggccgactat gctcctgtca
      301 aaggctgtat ggttatccgt ccttatggct atgccctctg ggagaatatt cagaaattca
      361 tggacccact tatcaagcag cacggcgtcg ataatgccta ctttcctttg tttatcccta
      421 tttcttattt tgaaaaagaa aaagaacacg ccgaaggttt ctcaccagaa ctagccgtgg
      481 tcaccgtcgg tggtggcaaa gagctcgctg aaaaattagc tgtccgcccc acttccgaaa
      541 tgatgatgta tgaaatgtac aaaaaatggt ttggtagctg gcgtgattta ccggtccgta
      601 tgaaccaatg gtgcaacgtt gtccgttggg aaaaaagaac ctatttattt ttacgtactt
      661 ctgagttctt gtggcaagaa ggtcattgtg ctcacttaac ttctgaagaa aatatggctg
      721 aagtcatatg ggctatcaag atgtaccaac agacatataa cgaattaatg ggtatgtacg
      781 gcatcattgg agtcaagagc aacgccgaaa aatttgctgg tggttcaaag acttatacat
      841 tcgaatcctt aatgcccaac ggtaaatctc tgcaggcctg cactagccac gatcttggcc
      901 aaaacttttc caaatcaatt gactggacag ttcaagatca aaaaggtgag aaggccatgc
      961 cttggcaaaa ttcttggggt ttttccactc gttctattgg cggtttgatc atgtctcacg
     1021 gtgatgaaaa aggtcttgtt ttacctccaa acattgctcc aattcaaata gttattgtcc
     1081 caattcctaa tcatcaaaaa gctttaagta aatcccaaga attgatatct caattaagtt
     1141 catatcgagt taaacttgac accgtagagg gtgaaactgc tggcgccaaa ttcaataaat
     1201 gggaactaaa aggtattcca attcgcttag agataggaga cagagatgtt gacagtaaca
     1261 ctgttatcat ttgtcgccgc gataccggtg agaaacaaac tgttcaactt cctgagttaa
     1321 atcaaataat tgagagtcta ttaaaagaaa ttcaacaaaa tttatttgag aaacataaaa
     1381 aatttactca agacaacact ttcactgtcg ataattatga tgacttcaaa aaagttatgg
     1441 agacaactcg tggatttatt aaagcacatt ggtgtgagaa tccagaatgt gaggcacaga
     1501 taaaaactga cactaaggca accactcgat gtctacctat cgacgctgcc aaagaagaag
     1561 gttcctgcgt ccattgcagc caaccttctc accatcgttg gttatttggt ctatcttact
     1621 aaatttcaac ttttagactt ttagactttt agactctata ctgtatctat gcctcgtggt
     1681 cgcaaaaaaa agaagttatt tcctagcctc catcttaagc cagatactat ctcgacgata
     1741 ttctttgtct tctttcttat tttgtctgtc ttgtcggtta tttcttttct tcagactggc
     1801 ccaattccca cccaatacaa tcttttttta actagatatt ttgggctcag ttcaattttt
     1861 accccattca tacttctctt aatagccttc tccttttcca agtttaaatt tcctctaaaa
     1921 gagcttaacg tttcgcttgg gtcattggtt atctttgttt ctgtaactag tttattccaa
     1981 caaggaatcg ctggtctttt cttttgggag cagatttcat ttttgtttgg ccaagttccc
     2041 gccttattga tttttatctt tacttttata gtgggatttg tcgtcctctt tgacactgat
     2101 gtcctttcta tttttaagtt cttggcttct gtatttatgg ttattaagaa atatactgtc
     2161 ggtaaagctg ctaaattatc taaggacaaa aaagttaaat cgactcaacc ggtttatatt
     2221 cagtctgaac tagatatcaa accagagaaa gaaagagacg acccggctgt cgtcaatatc
     2281 ccacccgttt tcaaatcaaa caatgcccct ttgccatcat ctactagtct tcactcgtca
     2341 cagtcgagta acgagagtca tatctggcaa tatccaccac tttctatttt tgatgacact
     2401 cctggggcca aggccgatag gggagatgtt cgtaaaaatg ctcagacaat tgaaaaaaca
     2461 ctcgagtctt ttggtattac tgcccgggtg gccgatatca ataacagtcc ttctgttact
     2521 cagtatgctc tcgaagttgc cttgggtacc aagcttgcca agatcgttag tcttagcaat
     2581 gacttggcta tggctttggc tgctcccggt ggacagatca gagtagaagc ccctattccc
     2641 ggtaaatctc ttgtcggaat tgaagtccct aatcgatctt tggaagtcgt gcctattcgc
     2701 accatcttgg cttctgacgc catgtctcag tccaaatcaa agaccactgt tccgttgggc
     2761 ttagacgtgg ctggtgttcc taaaattgct gatatttcta agatgccgca cgttctcatt
     2821 gctggtcaaa ctggttcagg aaagtcagtt tgtgttaatt catggatatc cactcttctt
     2881 tttagagctt ctcctgccga gttgagatta attatggttg atcctaaacg tgttgagttg
     2941 actccataca acggtattcc tcatttgtta acgccagtta tcgttgaacc agataaagtt
     3001 gtttctgctc taaagtgggc agttaaagag atggaagatc gttacaagac cttcactgaa
     3061 gttggagcca aaaatattga aagctataac aatctagctg gtttcacttc catgccttat
     3121 atcgttatcg tgatcgacga acttgccgag atcatgcttt tctccccttc tgaagtcgag
     3181 gataatattt gtcgtattgc tcaaatggct cgggctgtcg gtattcactt agtcttagct
     3241 actcagaggc catcagttaa cgttatcact ggcttaatca aagctaatgt tcctactcgt
     3301 attgcttttg ccgtctcctc tatgactgat tctcgggttg ttcttgatac tcctggtgct
     3361 gagaaattac ttggtcgagg agacatgttg tacaccccgc cggagttagc caagcctgtt
     3421 cgtgttcaag gttgttttgt ttccgacaaa gagatcggtc gcttgattga ctttctcaaa
     3481 aaacagaaaa ctaccgagta taacgatgaa attgttaaac aaccagtcaa tatccccgga
     3541 tctaaaacca ataatattgt ttccgttgac ggtgctgagc gagacacgat gtttaatgat
     3601 gctgtccaat ttattgctga cacaggtaaa gcttctgcca ccaaccttca gagacacttc
     3661 aaaattggtt atgcccgtgc cgctcggctt ctagatgaat tgcaggctgc caatatagtt
     3721 gggccagtta acggagctaa gcctagagaa atacttattc cccataaaca atccggagat
     3781 tcaggagatt tttaaggata aaacattatg tttcgagcct caaccattat tagaaatgcc
     3841 cgtgaagaca aaggtttatc aatagaagaa attagtaaaa agctcaagat aaattcaaaa
     3901 tatctttctt ctattgaaga tgagagagcc gctgattttc cggatgaacc ttattgttct
     3961 ttaatcatta aggactattc aaacttcctc ggcctcaaca gtgaggatgt tctttccttg
     4021 tttaggcgtg attttgccac ttcatgcaag gttaagccag ttacccactc aaaaatatct
     4081 ctaacgccac atcatacatt cagagtgtta actatagcct ccatattttt gtttattgcc
     4141 tatctatcat ttgagtacgt taaatttaac cgagccccat ctttaaaagt caactggccc
     4201 gatttaaacg gcgcccagtt agagatatct ggcattactg atcctgagtc aactgttcgt
     4261 gtaaacaacg atttgatact tgttgatccg tcaggatctt ttaagaaaac aatcaatgtt
     4321 gctaccgaaa gtaccgtcat tgttgagtct aagtcgcaga gtggtaagac gactgttgtt
     4381 gaaaagacct acaaatagtc tataattttt ttatggattt aatacaaata cttattgtca
     4441 tcagtctgct ggttgtttct gtttcagtag ctgtctgtac ttactacatt attaccctga
     4501 ttattgaact aaaaaagacc attataaaaa ctacttcaat tctcgatgat acccatctta
     4561 ttactagctc ggtcgccaag ccagtttcta gtttctctga tttcttaatg ggtttcaaaa
     4621 acggttttaa attatttaat actttctttg acaaagacag aaaataa
//
LOCUS       DDWP01000057            2761 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_32148, whole genome shotgun sequence.
ACCESSION   DDWP01000057 DDWP01000000
VERSION     DDWP01000057.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_32148"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          820 a          425 c          711 g          805 t
ORIGIN      
        1 ttatcataga cgacaatggc taatacgtta tttaaattct aaactctaaa ttctaaattc
       61 taaaattatg aagaacttac ttatagtgga gtcgccaact aaggcgagaa cgattgggaa
      121 gtacttgggg gcggattatg aggtgatcgc cactgtcggg catttgcgtg atctaccgaa
      181 gtcaaaaatg ggagtagatc ttaaaaatga ttttgaagtt gactatgtgg tcgacccaca
      241 aaagaaacag gtggtaagtt cattggtaaa ggcttcaaag aaagcaaaaa atatttatct
      301 agcaacagac cctgatcgag aaggagaagc aattgcctgg catgttcaat atcttttagg
      361 caatatacca atgtcgagag tgtcgtttca tgaaattact aagacagcga tagaagaggc
      421 tctaaaacaa gcgacgcagg tagatatgaa tttggtagat gctcaacagg ctagaagagt
      481 gctagacaga gtggcgggct atagcttgtc accaatattg tggaagaaag tccgtcgggg
      541 gttgtcggcg ggacgggtcc agagcgtggc agtaagattg attgttgaaa gagagaaaga
      601 aattgtaaaa tttaaacgag agaaatattt taaagttagt gctgacttag gttttattgc
      661 cgagttaaca aaggtaaatg ataaaagtgc ctacgtttcg gagaagttga ctttgtttga
      721 tggtgaatat agctttacta agtcgatttt tgaagatagg aagaaggtgg atgaatttgt
      781 aaaaaaattg gataaagagt ttgtagtcaa gacagtcgaa gggagagaag tgacgagaca
      841 gccgttgcca ccttatacca cctctaagct tcagcaagcg ggagctcgac gttttgggtg
      901 gtctggtaaa cagacaatga tgttggctca gagactgtat gaaaagggtt tgattactta
      961 tcatcgaact gacgctgtca atttgtcaac caaggcaata gaggaatttc gaagttttat
     1021 tagtaaagaa tttggagaaa aatatctggc agaaaaagca agagttttta aaaataactc
     1081 caaaaatgct caagaggcgc atgaggcaat tcgtccaact aatgtatcaa atagagagcc
     1141 ggatgatgtc gacgctaagg aattaaagtt gtaccatttg gtctggaaaa gggcggtatc
     1201 gacccaagca gcaccagcaa agatgcaaaa taccacagtc gaacttgtca gtggcactgg
     1261 actgtttcgt tcggttgggg taagaatgtt gttcgatgga tttttgaaaa ttgctggaga
     1321 aaaatttgaa gatcaaattt tgccagagtt gatagttggt caacatttga agtctaaaag
     1381 tgtgaaagtt gaagagacga taactaaccc accgccgcga tatactgacg cttcgctagt
     1441 agcctctttg gagaaacaag gtattgggcg accatcaaca tatgcaccaa ttatctcgac
     1501 ggtacaggcc cgacagtatg tagaaaaaga tgaaggaaag tttagaccga ctgtacttgg
     1561 tggtgcggtg acagaatttc tggtaaagaa ttttgaaaat attatgtctc taccttttac
     1621 agcagaaatg gaagaggatc tggacttggt ggctttaggg aaactaaagt ggaagaaaat
     1681 gatgaaggac ttttggagta aatttgaaaa agagttgaag gtggcagagg taacggcaga
     1741 tagagtgaag gtggaggtgg agaaaatagg ggagaagtgt ccggagtgtc agagcggaga
     1801 attggtgatt agattaggaa gatttggaaa atttgtgtct tgtgataaat ttcctgattg
     1861 taaatatacc cgacaatacc atgaaatggc tgggttcaaa tgtccgacat gcggtaagga
     1921 tggggtagtg aggaagacaa aaacgggaag aaagtttttt ggatgttctg attacccgaa
     1981 ttgtaagtgg gcagggtgga aaaagccgta gcgaaacaaa ttctaaaaaa cacttattgg
     2041 ttatcggcgt gagtagcttg aaggacctct ggtaccgact aggctaaggt tttccctgct
     2101 ggtgttattc tgccgagcaa tggtgctttc tggagagaat cttaattgtg gctgaacatc
     2161 caatttccct tcgctgatcg actttaaaac ttctgcagtt ctttgatttt tttctgctag
     2221 tatttctccg tatgtttttg ccatattagg ggattataac attaaaatta ttcacctaga
     2281 aaaccagggt tgttgaaacc atctttgggt tttttaggtt tatttgcctg gtgacgaata
     2341 actacgtttt ctattggacg agtgctatct tcagtgtgac ctgtgcctct accacctgtt
     2401 tggatgctgc cgcctagctc gccttttagt tgtgtttttg cccagtcaga ggagttgcct
     2461 ccggtagttt tctgatccgg atgggttcct tctaacatct ggtttaagtc gtgtcttctt
     2521 cttgcggcgg ttcctaaaag agtgttctct tcgacggttg gtctcccgtc cttgtcatcg
     2581 gtgatggtga tatggacagg tagacgtttg ggtctgtcgg ctggtttttg ttctgcctga
     2641 ttttgttttt ttaatagagc tagtgcttct gatgatagtc gtaccataat atgtgtattg
     2701 tatgtgtatg tttgtaataa gtcaataggt taggattatt ctttagtttg ttgacgagat
     2761 c
//
LOCUS       DDWP01000058            5707 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_32806, whole genome shotgun sequence.
ACCESSION   DDWP01000058 DDWP01000000
VERSION     DDWP01000058.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_32806"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1704 a          728 c         1490 g         1785 t
ORIGIN      
        1 atcacgagat atgccctcat tgtagccggc agtcaaaata ccaatggttt catcttgctt
       61 ggcaatataa gtgccgccat agccaacttg attattaggg tgaattgatt taatgagagc
      121 aattcgagag gtaaaagtaa gagcgatgcg gagagactgt ctttgctgga taccttgact
      181 agtgtgtgga ccaaagggac tatatccata gaaaccaaga cctagtcgaa ctaaattaaa
      241 atatggatca tagatagtgc tggcgccggc ggtagcggca atgtgtttat acttgaagct
      301 aaatccagct tgctcaaaag tagataccat ttctttaaat aattttattt gtttagaggt
      361 gaaagtggct tttcggggat tatcagcttg agaaaggtga gaatagatac cttcaatctg
      421 aaggtggcgg cattttttta attcataaat gaaattactg atatcttctt tctgaatacc
      481 tagacgacac atgccggtgt ctagcttaat atgaattttg gcaccttttt ggtgagcgtt
      541 gagtgatcgt aatgattcaa catcgtagac agtgaaagta aacgggagct tcttccagac
      601 ggaatagttg tgcggatcag tataacccat gatcatgatg ggagttttta tcctttgttt
      661 ggaaagttca taggcttcat aaagagaatc aaccataatg tagggagtag agatattact
      721 gtcgataatt ttggctactt ctgttagacc atgggcgtaa gcgttagctt tgagaactgg
      781 acaaataaag gatggtggat tcaatttctt gaagtagtta taattatgaa gaagagctga
      841 tgagtcgatt ataatttcat ttaaagtttg ataggcagcc atagtttaag atatcatttt
      901 tagttgacaa agtaaagttt gaatctaaaa acagtgttga caaacaatag ttataggtat
      961 aaactgaatt atgaataaaa tcagagtagg agtgcttttt ggaagtcgat cggtagaaca
     1021 tgaagtgtca attgttacag caatggaagt gtttgcggca atggatagag aaaaatatga
     1081 tgtagttcca atttatattg acaaagaagg taaatggaat acgggtaagg atctggataa
     1141 attggagtcg ttcatgaatt tacgtttggt gactaaagag actttgcagg agtgtatgtt
     1201 gccggcagta gtggggcaga agagtgtagt accagcagta tcaggaggat tgtcaaagga
     1261 aattgatatt gtctttccag ccttacatgg aacttatggt gaggatggaa caatccaggg
     1321 tctactggaa atggtgggaa tgccatatgt aggttgtgga gttggagcgg cagcagtggg
     1381 tatggacaaa gtattacaaa aagcagtttt tgaaaaggaa ggaattcccg tggtgaaata
     1441 tgagtggttt atggatgtgg agtggaataa atcaaaagaa gaaattatta gaaggctaga
     1501 aaataaactg ggttatccaa tgtgtgtgaa gccagcaaat ttaggttcaa gtataggaat
     1561 aaatatagcc aaagacaggg cagagttaga gtgggcgatt gaagtggcca aagaatttga
     1621 tcgtaaatta ttgattgagg aggggttggt aggaatagat gaaattaact gttcggtaat
     1681 gggagtagat gaattagagg cgtcggtgtg tgaacagccg gttaaagcca ataaagtgtt
     1741 aacttacgag gaaaaatatt tattgggagg aaaaactaaa gggatggctg ggttagctag
     1801 attgttgcct gcgccgatac cagataagtt aagagatagt atccagaatt atgcaaagat
     1861 ggcttttaga gctattggtg caagcggttt atcaagaatt gattttctgg tggatattaa
     1921 aaaagaaaaa gtatatatca atgagattaa tactttgcca ggtgggatgt ctttttatat
     1981 ttgggaaaaa tcaggttata cctattccca agtggtagat aaattaattg atctaggttt
     2041 tgaaagattt aataaatacg ctagactgca aagaagtttt gatacaaagt tgctgaagac
     2101 caatagtggt ggggcaaaat ttcagaaata gttgtcgggt aaatcgtttt cgaagaggac
     2161 agcagtatcg ggagaagtaa ttctgggtaa taattccagg gtgtcgttag tgtgatctag
     2221 aaggtggatt gtggcggaag tgttttttaa tccggcaagt aaagcttgtc tattggtgtg
     2281 gccaactatg ataatgtgtt ggcagaagct accagccatt ttggcaagac gagtattttc
     2341 cgtggcttga atatcaccta gctcgatcat accgggggta actaaaatat tatttttaga
     2401 ttttagagat tttaatttat cgagagccat agcaaagcca acagggttgc tgttgtaggt
     2461 gtcgtcaata atattcatgg agccgttttt gatgatttcc aaacggtgat ctgaagaggc
     2521 gattggacta atactatcta tatctagcag tcgagcaaga gactgataaa cgtcagttaa
     2581 ttgtttgaag aataggatgt ttttagtttt tatggaaaag ttgataagag aagaattttt
     2641 ttggtcggca ggcagataga gatggccggt ttttggtatc gattcagcta gttccatttt
     2701 ggttttgcgg atattggcaa gagagccaaa acgttcaagg tgcatagagc caatggcggt
     2761 aataatacca atttgagggc taactaattg gcatatttgt ttgatatcgc ctgggtagta
     2821 ggcgccggct tcacaaatga aaatttgcgt atccacaggt aattttaata tccttaggct
     2881 aacgccgata agggtgtttt gattggctgt cgtagcggca gttcgatatt tttttgagag
     2941 agacgggagg agagtttctt tgacggtagt tttaccccaa gagccggtta tggcaatggt
     3001 ttttagttgg cgatggaaaa taatgagttt gactttagcg atcaaaacta ggatgtattt
     3061 tgatatatta tcgataggtt ggattaggtt aagggtaaga aaaacagagg tttgtgaagg
     3121 gaggaaaaat aataaagacg agatggtata gagaattttt atttttgtag tccagactag
     3181 actcttttta atttcaagga cttcacgtcc gggattattt ttaagccaag ctttaacacg
     3241 gttaatgtcg tattcttcga gttggactag ataaagaaag tttttgagtt tcataggata
     3301 aaatcagtaa ttaattggct tacttgttgg gggtggtcaa gataaaaaaa gtggccagta
     3361 ttagggaaag atttgaactg tgagctttta ataagagagt ggattttttg accatcggca
     3421 aggggggtgg cggcatcgtt gtcgccccag agaattaaag tgggtgtggt gattttagat
     3481 aaactggttg attggtcagc atgaataaga tttctaaagg tctgtttttc aatatcagtt
     3541 ttgaggacaa tgtagtcgga agcgtcaagt tttaggagtt tgagaatggg ctgacgaagt
     3601 ggtcgtaata gtttggagaa cctctggaag aatttgatat gggaggggag ggcatggcga
     3661 actgaggcgg cggagaggag gataatctta gaaatggtat gaggctggtc aacggctatc
     3721 ttggtactga tagcaccacc cagagaatgt ccaattagaa ttgggttttt aattttttct
     3781 ttttttataa aatcgaggac gaaggtggca tagtcattca aagttagcac tttgtcttta
     3841 ggataaggta ggttgggatg atagacgtta aaggacgatt caagtgaatg aatgatgggg
     3901 taaagagatt cccaggtggt cccccagcca tgaagaaaaa ctagattaga tttaggcact
     3961 agagtatttt aacaaaaata gttattctgt tttggttaag gagtcgacgt tgacgatcag
     4021 attttctact ccttggcgaa ttattttttg cgagtcttcg atggcctgat tgtatatctc
     4081 agtgttgatt ttttttaata ggaaatcaag aatttcttcg gcagcaataa taccaatatt
     4141 aatatcgtgt tcggcagaaa aatagtcgac aagctcttgt ttcaagagtt cgcgtttgtc
     4201 tggagataag gcagaggtgg ttttgttcat attatttatt aaaatatttt ttggcctcga
     4261 tggtagtttt agtaatgaaa tcggtttttg ccttagtgta ggcttctctg ttatgtgaat
     4321 atttttttag tgattcaatc ttaattgttt catattgttt agcaaggttg ggttttaact
     4381 ttagataatc tctgaaatat aacctatccc aaagacttga gttgtttttt accatatgaa
     4441 tatggtgggt cctgtggccg tgtttgttac gtttgataaa ccaggagtaa tagggtggct
     4501 tatcaaattc tggtcgccag aaatagtcgt aactaaggtt ttctaagacg gggacaattt
     4561 cggttttgac ttctttaaaa ctagttattt caacgagtag atcaacaatt ggtttggcgg
     4621 atagaccagg tatggcagtg ctaccaaaat gttcgattcg tttaattatg tggggaaaac
     4681 tttttgtaag aaacgtagct tctttttgaa atgaatttga ccattttgga ttgtaaggaa
     4741 caatgttaat tttttctttg acaactctat cgattttggc ttgaagtgtt ttatcggtgg
     4801 ccacaggtag agtatatcac cgtgtctatt aatggaataa ctgttctgtc cgaagggcga
     4861 ggccggagtc aaaacattga tattttacct gttttggtgt ataaacagat taattatggg
     4921 tataggtaaa ccagataatg aggtgctggc agacatactt agcggtagtg ctgggaagtc
     4981 ttcgcctggt aaagatgaaa agtggtctaa tggaggtgtt tttgccgggg ggagagtcag
     5041 acgagaagtg tctggaaata atagaagggt tatcatggaa ttacctgaag gtagggggaa
     5101 gttgactttg ttatcaaggg tgggggatta tggtcaagat gtaccaatat cgatggttca
     5161 tgagcgtggt ggtgttgtaa ggacggcaaa gtttgtagtg acaaatgcag ctcatcaaga
     5221 ttatcaaatt gagaattcgg atggtagttc tgctggaaat gtgtttttgc aaggtgctgg
     5281 gagatcagag atgagtatgc gtgtagctaa gggtctttac tcagaggatc catgtttatc
     5341 aaaagtggat ggcagttgtg gcccagcaag tgtttatttt tgggtggaga agtagtcttc
     5401 tgcccggagg gcgaggccgg aagcgacgga agtgaaatgg tcagaagtga taagtttttc
     5461 aggaccaaag atctcagata tcaatttgat aaaaataggg atttgtgagg aaccaccagt
     5521 gagaatgact ttgtcgatat cgtttggttt taattgagct tgatgaagtg attcggtgat
     5581 gcatatttgg gtttcttcga gttcgttgac gatggcttgt tcaaagtttg tcttgctaag
     5641 agtttgtttt aaattaagat gtggccgatt gaaagagaaa tcgacagtat ctagggtgga
     5701 gagattt
//
LOCUS       DDWP01000059            3800 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_34014, whole genome shotgun sequence.
ACCESSION   DDWP01000059 DDWP01000000
VERSION     DDWP01000059.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_34014"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1161 a         1019 c          495 g         1125 t
ORIGIN      
        1 gatcagcaat acattttctt attttcacca gcaaatgttc ttgtttatta ataaaaccac
       61 cactaaaaat caccgcatct gatttaaaat gagccaaagt attaattatt ccctgggcca
      121 tcctttgtga aactatattc cacacagtat catctaccga caatttttcc ggaccaaccc
      181 catatgcaac ctcaaaagca cctcctccca cataacttga caacctaccc cgaggtccat
      241 gtccaaccaa ctttccatcc cattcaataa tctggctgcc aatttctaca ggaaaaacaa
      301 tcaacttatg atccaccctc ttaatcatcg cccctcccac accagttccc catataagac
      361 atatcaactc atctaccttt tttcccgttc ccaagactat ttcagcaacc gctgcacaca
      421 ccgcatcatt ttctattttt acaggtaatt ttatcaaagc cgacaggtca ttagccaatt
      481 ttttacccga ccaatcaggc aaattgaagc tcaataccag ctccccagcg acaggatcga
      541 caacaccagt tatcccaata cccacaaact tgaattcata ttttctctta tattcattaa
      601 taatttccgc tatattacta attccttttg cataatcagt gtcagtctta atagacctct
      661 cctcgagcaa tttataatca tcgctcaaga ctccaaattt tgcgcttgtc ccaccgatat
      721 ccactccgat taacatgttc agattctaac acatcaccaa ggtttagaat ttagagttta
      781 gattttagat tttatgctgt ataatcctca atatgctcaa aatcaaattg ttccccaagg
      841 ggaaaaaaca tcagataact ttccgtatcg tggttgctga ggccagaagc aaatacaacg
      901 gcaaatttgt cgccgattta ggttttgtta ccccacagac caacactgtc cagatcaaca
      961 gagaagaact cgcaaaatgg attaaaaatg gtgcccaagt tactcttggt gtcgataaaa
     1021 ttttaaaccc cgacaaacac gtccgccccg ccaaagtcaa acccgttaaa gaagcaaaaa
     1081 cagaagaagt taaagaagaa gttaaagttg aagaaaccac tcaagcataa tgaccaacat
     1141 tgctgattca ttagactacc tcgttaaagc tgtcgttacc gattcatcat ctatcgttat
     1201 agatagtcga gaagagaacg gcatcatcat gtttgaaatc actgctgccc cagagataat
     1261 tggtcaaatc attggcaaag aaggtaaaat aatcaaatca atccgaacta ttcttaactt
     1321 gtctttccct tcaatccgct actctctcga cgttaaaggc taaaaggatc aagccttcca
     1381 taatccctaa actctacacc ctgtcctttt tgcatggtgc tagacgacag attatcccat
     1441 atcgtatacc caccaatctt tgacaacaga tccgactcct ctttctttaa tgacagttcc
     1501 gacaaagtca atttatttat atccacctta cttcgaccct caacgtaaaa cgccaacact
     1561 ttaagatcaa gtcttgacac tccgttatca cttctcatat caaaagtatc tattgataat
     1621 atcggcaagc ttgtttccat accttcaatc aattccagat gcctcaacgt tggaccaact
     1681 agcgataagg ttactggtat tttgaccact ccatccattc ctgccttagg tgcttcacta
     1741 atcttcccag gattaatcac aaaactatcc aattgaaacc catatttgct ggctactttt
     1801 cccaccaccc cgaccaggaa ataagaatta ctttctggca gtaacgcgct tgatataaaa
     1861 tcagcatctc tctgaagatc ttcttggttt acagactgca gatatttcgt cttttctacc
     1921 gtcctagctg tttgattctc catagtagac aatctactat ttaactcacc aaactcctca
     1981 aacttcggga caactactaa attaacgctt atgataataa acaaaaccaa cccaagcaga
     2041 ggtacaaaag ctccaattaa tgccgaatca ataccaaaag ttttctccgg catttctaaa
     2101 ccatacattt tcatcttaaa ttaacctcca ttacaaattt ccaaccttgg ctcttacctg
     2161 tcgacagact caccagtacc gcctcagaca acccttctct ctctccagca ttaatttcct
     2221 caattatctc ttctagacga tctactccct cctgagttgt taatgatccc tccactttac
     2281 acacaccact ctcacccaat tcatacttat caagatttat tccactatca aataaaacat
     2341 ttataatttt aaaagactgt ccatattcaa accggttacc caacacccca gccaccaact
     2401 tagctttatg cttcatatct tgagtcagca atatactatc cgacattagg gcataactat
     2461 cagtcacact cttattcttt gctgtggcag tttgaattct catacctagt acccaatttg
     2521 tcacgaaagc aactatcaat aacaacaacc atcctcccgc cagccagatc attaccattc
     2581 taatcctttt ttgtagcctt atcttagatg cctgaaattt agcactcgaa ggtaataagt
     2641 ttaagctcat tgcactcatt gattccccct taaggccagt cccacagcca aactaaatcg
     2701 acaaccttct gcccccaggt caattggcaa cactagcttt gtagtatcta tattagaaaa
     2761 aggctggata acttgtatct ccactcccaa atttttagtt aattcttcag ccaatcccgg
     2821 aaggttagct cctccaccac ttaacaccag aagatctatc gatttacctt gttcactttt
     2881 aaacagttca agcgcccgac gtatttcgtc agtcatgcta gaaaacaccg gaataatcgc
     2941 attttttatc tttccttcta gctgcatttc tttcatacca taggcttttt tatattcttc
     3001 agccgaggcc atatctaatc ccaaattaat tgatatcgcc cgagtcaatg actcaccacc
     3061 gacagacatt gtccgagtaa aatatatatt ccctttattt atccccacca tgtcagagta
     3121 cttttctccc atgtctacca acaaaattgc tgccgtgtcc gatactgatc taactaccgc
     3181 tcttcttatt gccacagcag gggactcaag agccaacaac tccagtccta ccgatttaaa
     3241 taactttata taagcctgaa tcaagtcatt cctggccgca atagcaaata tagtcaatct
     3301 tcccgaagag tcttcctcta ttacttcata atcaattgac acttggtcaa gcgggtaggg
     3361 gacaaaggtc tctgcttcaa attttaaagc atccttaact tcactttctt ttaacggcgg
     3421 taatcttatt aatctagaca ccacctcatc ctcaggcaag ctagtcacta ctttttttgc
     3481 attcacttta agatcggaaa gcaacatctt caaagaagtt gtcattgccg ccataccctt
     3541 gccatcttta tcattcctta tccattccac cacaggagtt ttggtctcac ccaaagattc
     3601 caaccacagt cccgacgaag actccgacaa agctacgact ttgatgcttc cgataccaat
     3661 atctatgccc aaaaattcac tcatttaatt agtataaaca gttctcccta gttactcaat
     3721 acatctccaa aagcagttat tgcttccaag gcaaaacttg gcacctggta tgtttgaatt
     3781 acattaattt tacgtttcac
//
LOCUS       DDWP01000060            3359 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_51844, whole genome shotgun sequence.
ACCESSION   DDWP01000060 DDWP01000000
VERSION     DDWP01000060.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_51844"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          879 a          748 c          511 g         1221 t
ORIGIN      
        1 atctaacttc caacctaaaa ggggactctc tagatatcca aaaaataatt aattactcta
       61 agttgtattt tccgggtctt gtcaacaaat tctatgttcc ttctgataaa aatatcgagc
      121 ttaagtcaac tactgagctt gacaaaacag cctacaatca ggctgaaata gctcaatcac
      181 taaagaaacc gaccaatcta cctctaccag tcttaggtgt tataataaat ccatctatgg
      241 aaaataaaaa aaatctcctc cccttcattg ctgtttttgt aactacagca gccatcgctt
      301 caattattat ttggtttgta cttagtaaga acccgagtac agatattgaa aacccaagta
      361 tcgatattga tgctattcca acttctgtcg ttcaagcacc taccgaagcc ccgactccta
      421 ctccagtaga gatcagtaag actctcaagc ttcaagttct taatgccact gatattaatg
      481 gtcaggcggc tatcctaaaa gagaaattaa ctgccctcgg ttttactagt gttgccgtcg
      541 gcaatagtcc tgagaagttc actgagaaca agattaagac caaagcggct agtagcactg
      601 cttctgctta ctttgatcaa gagttaggtg atttctttac tgccacttcc acctctgatc
      661 tttcagcatc ttctacttac gatgtagttt tctatattgg taaggatctt agtggtaaag
      721 cagccgctac ttctaccgct accgccactc ctactaagaa agtaaccgcc actccaacag
      781 tgtcagagta acctatttat aaaactaaaa aatccccctg cgaagaggga tttttgttta
      841 tattactctg atatcttgat acccatgctt tcgtatttac gcctacttat accccattct
      901 gcttccgcaa tccaatcact agacgggcaa tcacccaatg caaatttcaa ctcttttacc
      961 cgatgtcctt ttggctcaag tatttgtccg gcaggttcaa taattgcttc tagcaacttt
     1021 tgtttttttc cccaatcact tggttctata aaattttggt ttgcaaccac agccaccgct
     1081 gttcgtgctt caattaatag gttaatggcc cctatttctt tttggcttag ccttttcata
     1141 tttttattta atttttaatt tgtacgtctg aatatttcaa ttgcatcttt tccaaatttt
     1201 cgtaatattg gtagtgcatt ctctacttta taacaggtta gtaatgtccc agccgatact
     1261 atttcagcac ctaacgggtt tccatttctt aaaaatagtc cgctaatgat aatacctcca
     1321 gctaaagtca ttacagcata acttccggcc acgtcacctt caagtcttct agtgaacacc
     1381 tctttgacat tggtttttgc ttgatcacca acatttctta aaaaatcttt catactttta
     1441 tttaattttt aatctatatc cttgtttttg gcacatcact aatttttact tgaaaacagt
     1501 cggttaatcc tataagtcct acgcttactg ccatataggg ttgccctgtt accagggcaa
     1561 tggttgccgc aattggatat ccccatcttg cagggtgatt ttttttgacg cttatagtaa
     1621 attgtttgtc tagaacctca attttcttca tatttttatt taatttttaa tattttttct
     1681 ttgtaacttt gtaagttatc aacggctcga ccaattggtg tacttcttcg tacaatcaaa
     1741 tcggtgactt gttccaccca ttcacttcct ttttctagtg tgactaggtc tttgacaatt
     1801 cttccaactc ttcctattgg tagttgggaa tctctctcga tttgatctcg cctttcgagt
     1861 aactgttttt gctcaccaac atccaattga gattggtatc gcagatcaga ccattcccca
     1921 aagtagaaat ccaaatactc ctgggctaat tttgttgcca cctctcgctc catataattt
     1981 atttgtttgg tcgactttta acttctgcaa gaagtattct cagattaatt tccaggtcat
     2041 cttcgatttc ttctggctta gaaaagtcat ggataggtat tatcccgaca gtttgaacac
     2101 ccccaattcc tgtttgctgt aaatataacc cactgattct tctcactgcg acatcctctc
     2161 ctgcgttaca ctctatagac atctctgccg gaagtgttac gcccataaaa ctaggcagat
     2221 cttcataatg tagaccaacc accttagatc ctgggtctgg agtatctatg attaccacct
     2281 ttcgtttagt atgaatcaac tttccatcaa tctttatttc tctcatacct ttctttaatt
     2341 tttaataatt aatttctaat tttgtttttt tgaatccagt aaattggcta ctgccttaat
     2401 atgcatgcct ttgacaaact cctgacccat tttttcgtct aattttaggt catgtctgat
     2461 ccaccatatt tgcccgtaca tatcaattag ttcttcgtgg cttgttccaa attcctcaaa
     2521 aactgctcta gcttcttcgc ttatttcgat aaaacgatat tttttattta gttcatcact
     2581 aaccacttca tcagtcgcat tctctatata caagtcaaat tgaaagacta tatgtgccag
     2641 ttgcattgat aaatcgatga ctctcgcatc tctaagagag tctttgcttg gctcacgtaa
     2701 aattcttgtt ctgattaatt cttcgtcact tcggctaaaa acaagtttat cctctgactt
     2761 attaacacgt gctctgtgtt cattccgaaa tttaattttt tctggactca agcttgttct
     2821 acgttctttc atatttttat ttaatcttta gctttattaa taattcttct ggcaattgtt
     2881 tggttatctt ccttgatagc ctctatggcc attctcaatc cgatttcttg aagtaatgga
     2941 tcagggttat ttattaacct ctttccaaat ctttctttaa ggcttactgt ctgattgtgt
     3001 ttcatgtttt tagtttttaa caattaactg acagatgatt tctaattttt catctgacac
     3061 ttgttcttct tcataataat ttcgcaatat catgtcagcg atggatgcca actctggcga
     3121 aagattagat ttctccacca gcattaatat ttctttttta gttaacataa tttttattaa
     3181 aaacaggggg gggttaactc ctgctttatg gtaaaaacta atttttaatt ttgtctctgg
     3241 tgtatgtgat gttgcgcggc cttgcggccg caaaactaca tacgccagag acgtgacttt
     3301 tctttgatgt attcccccac gtccagggtt ccgggcttgc gctcggaacc gaggagggg
//
LOCUS       DDWP01000061            4567 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_38431, whole genome shotgun sequence.
ACCESSION   DDWP01000061 DDWP01000000
VERSION     DDWP01000061.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_38431"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    3925..4123
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1230 a         1173 c          676 g         1286 t
ORIGIN      
        1 ctctgagtcc ttgccttaac taccaatgct tttgctacta actttttacc agtcctattg
       61 actgtctttt tgatttcttt tgccatagtt tttattgcct aatttggggt ataaattaac
      121 ccaaaactat tttttaaaat ataaattttt aatccaacga ttataacacg gttatcccac
      181 taatcgaagc tgacgacgat tattacccat acgtaacacc ggcgtttgta atttacttgg
      241 cctctcgctt ttccagccac gacgttgcca gcacagatcc atatcaacgt taaatttaac
      301 ctgttctaaa tcagataaag cttctatttc cataaaatgc atccttaaag tcgaggagtc
      361 acaccctgta gccgccatcg aagccgctac tcgacgatcc atcaaagaat ctagcatatt
      421 ggccatcggt ccaactaccc cagaccaccc atctgccaac atcccaatca cttgnnnctc
      481 attggaaatt gtcttacact agtcaatatc gaaaatccaa tatatgatgg acagccagtt
      541 gtctcttgaa cagcagaaga catcgccaac atgccatcta tctgtccatt agcccaagta
      601 ccagtaatcc gactacttaa gacaccttct tccacaccac tatatctaca aactccaaaa
      661 aaatccgcct ctctatatcc aagccaatta aaacttgtct tttcaactaa accaataagt
      721 ctttgagtct cctccggatt taaaaccaat acctcaccct gactcctctc ttgccattta
      781 accggcagta aagacttatt ttttagcttg ctacgttcat tcatgaaata tgagaacgat
      841 taactttttt gtttgctatg tctcgctacc acatcctgaa aaagagaact atcacccagc
      901 acagcttgcc tttttttgat ctgatctttt atggctaaag cagcttctct ttcctccctc
      961 atcactcttt ccagcacttt tttttgattc gtgttagtta cactagacaa agctatctca
     1021 gccttcttat aacgtctacc tctctcaacc acttcttcta caaaaatcgg aataacggct
     1081 cctgcttcca actttcttgc ttcttccatt accattttcg gtcgaatcat tactccctct
     1141 cggctatccg gtgttcctat tactgtcgcc gctacctctt caatttctgc taaagaatcc
     1201 atagccattt ttatttgacc taactcttgt aaaactcttt taggtgatct acacaaaatt
     1261 ggcggaattt ctatctgccc cctgttaaca tcccccccag caggcttaaa aacaatcatc
     1321 cctaccaaat cgtttaattt agatccacca acaaacggtt tagcccaatt tggtagtttt
     1381 ttagatacag ttactgcaaa aggtgctggc ttaacttggg cgacatagcc atcccttgga
     1441 gcatctccgt gtgcatctag ctcatcaggc aaaatcacat tttcccatcc caaagtaaaa
     1501 gaacgcggtt caacaacatg gtcaccaaat tggtcagtat catcagcgtt aatcgtatat
     1561 gtaattccct taggaccaat cttgtaagct gccgactttg ctacaggaac ctctaatggt
     1621 gaatcagtca tcggagcatt ttgtaccgct ataaattgag aaaacttttc tttataatca
     1681 ggaaaagccc aaccagttaa actatcaacc acaattgaac cgttcggtac tgaattacgt
     1741 attttgtgga ccatggtatg aattctttga taatctgcac caaacttcat cttcatctct
     1801 gccaaagata aatcttgcat cctcgctaac aggtccactc ttagactttc gtgagctgct
     1861 gcttcacctt tttcaagcca cgtcaatgcc aattgtgagg ccttttcttg ttccgcttga
     1921 ctcagaggag cttcaattcc aagatctgtc ggtaaactta gtaacaaatc caacttattt
     1981 tctgcatccg ctgcaattga tctattcatg tcatatctac ttaatatgac ttccccccct
     2041 gggtttaatg ttcccacttc caccacacct ttttgaacct tttttgcaag atagttaaaa
     2101 gtttcaaagg ttggcatctt aataacgtcc ttagatccgg ccgacttata cactttgtta
     2161 acgccaccaa gaaaccattt cctctgcatt ttatccaaaa cagtttcttc ctcacctgct
     2221 tgagtcgaca ataaatcaaa agctaccgct ttgacagcgg aactcaccaa ccctggcatc
     2281 ctactcttct ttttactttc tcccatcaat ttaaaatctg tctttctagt tgacggcacc
     2341 atatccacat cctctctttt ctgacccaca atagcaaaac tagatggtcc actctcccca
     2401 gcatcaacca catcaacatg tctactacca atcatttgga aaccagaact tccgctacca
     2461 gttctaatga cggccaacaa ttggtcacca ttaagtcctt cttttccaaa tgtatataac
     2521 cttcccatct ttttatttat taaacattaa ttaataaaat ttattatctt tttacctttt
     2581 ttttaactgc atttcgcacc aaaactccaa ggatatctgc tgcagctgca ccagttgctt
     2641 gatgagccga tgctccacca tccaatgccg ccctcccaga aggtgtcgaa cccacaattt
     2701 tatctacaag tacagcggca aaaccctgac cagcctgata aagtggacta gattcttttt
     2761 ttaatctctt ttcatcttcc tcttgaagtt tcatcctctt cctctccgcc ctaacctccg
     2821 acactcttcg gttaaaatta ataaaaccat cagtagtgat ggctgttttt ataactcccc
     2881 taaccgttgg cttctctggc actcttaaaa ctgctgctgt gccgtccttg ttatgagcca
     2941 acacaatatc cgtctgagct tctggccctc tactaccaaa tattacaaga ccggttttgt
     3001 tattaccaga cattatttta tttaattttt aatttataga tttcgccgtt attccggctc
     3061 atcagtatca gacattgtat cattaaatga tacaaactga tatatctact acacacaccc
     3121 acaactacta caggcctgcc ccttataggg gcaggccaga gttgcaggta tgtgcagaaa
     3181 cacacactat ttgttgacgg gctggtaggc ttcccaaaac caaacgacgt cgttgaagct
     3241 gggcagacca actgtgacca cctgagtaaa aaaccagttc aggaagaaca gtaggactac
     3301 cacaaccacc acaatcatca cggtcgtgat cgccaaagcc acaccgcgag aaggggcatc
     3361 caaaatcacc gaaatcgtgc cgagaatcgg cgactcgata atggcatgac ccatttcgcg
     3421 aatcctatcg gagttcatgg caagtaaaat gataattacc gccacaatca aaatccaata
     3481 gttggacagg aagttgccaa cattttctaa cataactttt tctctcctat ctttgtatta
     3541 tcccatgatt gggtattacc gggctgatgc tcaaaaacat tccaacaagc accattcaaa
     3601 acaccctgct gctacacaat agcagacttt taagttaaca gcccaaaatt caggctgaaa
     3661 attcatttgg tacttggcat tactgcgcaa tatataacaa cggcttgaac tcgttgtaat
     3721 attgctcatc cgtgaagcca cagtccccat aaacggaacc acaaccccac cgggaaagaa
     3781 actccatgat cccaaccgct ttcgcgccgg gataaaaatc cagagttcga cgagttgcgt
     3841 caaactttgt ctgagcacac ggagttccag aacagtgccc gtcaaagatg ggtataacga
     3901 ggactttacc ctcgttatag gaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaatgag gtagctcaca
     4141 tcttggtttg gtgtgatgtc caccaggaca atacacttgc cgatacaacc agcaattacg
     4201 gcgtcaatat cccgttcact tttgttaaag cggatattga ccaccggcac gacattacca
     4261 gctttatacc agccgcgagt tttctcggca taggcttcag ctagctctat cgccccttcc
     4321 ggagtgctgg tcgtccccaa cgtcccccat cctgataacg cgccatatac cccagaatac
     4381 agggtaccgt caccttgcgg caacaagctc gttttggatg aagatgttgt aggttggact
     4441 agtccaactt cctgggctag aacattccaa tctactttga tcccaaaatt accgataaga
     4501 accaggatca aaccgactag caatgttccg aaagccacct tcccccctaa taaagttgac
     4561 acaatca
//
LOCUS       DDWP01000062           10274 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_20558, whole genome shotgun sequence.
ACCESSION   DDWP01000062 DDWP01000000
VERSION     DDWP01000062.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 10274)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10274)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10274
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_20558"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3271 a         2463 c         1584 g         2956 t
ORIGIN      
        1 agatggccta ctaatctaca accctgacga tcaaaatatt caatccctcc taccattttg
       61 taaaggcaaa gttattccct atatcaaaaa agatttcccc accactttag tcggcaaaca
      121 taatcaagaa aacatttctg ccgcctacac tctttgccac catttggggc tagactttga
      181 tattagtgga tttaaaggca taaaacgcag actagagatc gttaggcaaa aaaatgacat
      241 acttgtcatt gatgattttg cccaatcacc agagcgagtt attaccgctc taaaggctgt
      301 ccagttctcc tatccaaata gaaggacttt tgtttatttc gaacctcatg cctctttttt
      361 acgataccaa caatcactta ctgagtttac aaaaataaaa gaatacatcc atcaattcat
      421 cttgggaaaa ataaaatttt cccccaacaa agataaccgt gtaactgcct caaattggaa
      481 agaatacttg ggagacaagc taacctacat tccgatagat accgacatag ttgaattctt
      541 ccgtatcaat ctacaaccaa acgacattct gattcacttt agttctggcg gtttagacgg
      601 actcaatacc ctaaaaacag tttataataa tatctaactg tgactcatct ttacctagga
      661 ctactacttt tctctttttt taccaccagt atattggtca taccttttat caatatactt
      721 tatcgcctca aatttcagcg acaaaaacaa accacccttg acttccaaga caaacgtacc
      781 ccaatatttg ataaattcca tagtcaaaaa tcaggcactc ctgtcggcgg cggattatta
      841 gtcgttatta cagtatccat actctttgcc ctaatcttcc ctcttctaaa actttcaaat
      901 atatatatct ccagtaacta ccaaataaat cctgaagtat ttatcatttt cttcaccttc
      961 atcagttttt cgatactagg tctatacgac gatattctaa agttctttgg ctttaaaaaa
     1021 agtggttttt ttggtctacg actaaaatac aaactactct tacaaatact gatctctgtc
     1081 tttattgcca tcttcgtcaa cctatatatg ggtattaact ttatctatat accatttctc
     1141 ggagcagttt cactcggtcc actagccatc ccactttaca ccaccattat cgtcactttt
     1201 gccaactttt ttaacatcac tgacggcctc gatggactat cttgcggatt acttatgatt
     1261 gccctctttg ccttttggtt tctttctagt gccagcctcg atactccgat ctctctattt
     1321 ctctcacttt ggattggttc tcttatcgcc tttctctatt ttaacgtcta tcctgcccgt
     1381 ctctggcttg gagacgtcgg atcactctcc tttggagcca ctattgccgt cattgctatc
     1441 ctcttaggga aactagttcc attaattatt gtctgttcac tgttcatcgt tgaaggtctc
     1501 agtagtgcta ttcaaatatt tagtaaagtc tatttcaaaa agaaaatttt ccccgcggct
     1561 ccaatccacc taactcttca aagagccgga tgggaagaac ccaaaattgt tttccgagct
     1621 tggttagctg gtattatttt agctattttt ggcctatggc tgggattaag ttaaaaactg
     1681 attttgccaa ttatcttggg attccctact ttaccaataa aggtaagtac aaaaatcatg
     1741 gtgaaaatgt cctagttggt aaaggtactg ccaaagaaat tgctctaaaa actattgagc
     1801 tagctaatga gcaaaatatc aaattaatcg aacttagttc acaacaaatc tataattttc
     1861 agaaaaaaaa tcatcttggc atagattgtt ctggattagt ttgccactta ctcaacagag
     1921 ggctagacgt tcgcaaaact tctgccaaca tgcttacttc atcaccactt gccaaagaaa
     1981 tatccgtttc aaaagcaaaa gaaggggatt tgattcgcca aaaaaatggc caacatgtac
     2041 tctacatctt agagaaagat ggtgacatta ttcactacat tgacagcagt cgtgaaggta
     2101 gaggcgtccg cctcggccaa ctagatgcta cttctcttca atcagttcac cgtatacaag
     2161 tggctcgata gattgtatct ccacctcagt accacattct tgacactcta aaatatctcc
     2221 tacctccatg ccttgtggct tagtaatttc tacctcacaa tcaggacaaa taattttatt
     2281 atcttgtgga ttcattttga ttcaaaaaaa tatataatta acctattata tgacaccatt
     2341 agagaatttt ggaccagtct atcttaagcg cgaagataaa aacctcaccg gatcagctaa
     2401 agacaggagc ttagatctac aaacacagca aaatatcaaa aaagccgtta tctcttcttc
     2461 aggtaatgcc gccatatccg cccagtattt ttgcaaactt aaaaatatag acctaaccgt
     2521 tttcgtctct cccaaaacca acccccacaa actaaaacta atcaataact acaccacctc
     2581 aaacaaacct gtctctgacg cttttaagtt tgccaaaaac accggtgcct actttcttcg
     2641 acaatcaacc gaccctattg ctctactcgg ctatcaacaa attggcaaag aactggtatt
     2701 acaatgtcct aagatcacca gtctcttcgt tcctgtcggt agtggcacca cccttttagg
     2761 catatcacaa accctaccta aatcagtcaa aatattcgcc gtccaacctg catcttattg
     2821 ccccatagct tcacattttg acaaaaattt tacccccgaa accaccaccg taactgacgc
     2881 tctctcggca aaattattac cattaaagtc aaaagttatt tctgcctgtt tttctggaat
     2941 tgtagttcaa gacttagaag ttaaaaatat tatcgatact tctcccgaag gtaacttggc
     3001 ctacgccggt taccttaaag ccattaaaaa tcatccagta ggggactatc ctgttatttt
     3061 attaaccggt gccaaaagat gaatttactt gatcaactcg atcaatccaa ctacgacttt
     3121 tatttcttag tcgtcgacga actcttagac atatctttac ctgaattaaa aaactttcac
     3181 tctctgtcag cccaaaaact aggtattgag atttctgaaa aaaactctgg acgcctgctg
     3241 tctcacccca ccactcaaca atttataaaa aataattctt caactactgg ccacacaccc
     3301 gtcatcattc ccttcaaacc atcagccaag attgatttaa tttgtcaaca aaataattgg
     3361 attttagccg ccaacccaac acatctcaat cgtttttttg aagacaagat aaaattttat
     3421 caatcttttt ctcatctccc tcttatcccg tctactatta tcaaattaac accagagaat
     3481 tactctcaag cccaacagaa atttggaagc aaattagtta tccagaccca ctttggctgg
     3541 gccggtaaca gctcttatat tagtcaaaac tatcaggata taagcgagct cattaccacc
     3601 ggcactaccg tcaagatatc cccctattta gaaggttatt cactaattaa caactgctgc
     3661 cttactcagc acggactcat ccaaagtcca cctggactac aatatactgg cctatctcaa
     3721 ataacctcaa acccattggc tacagtcggt cgccagtggc cgtctttcac tcctcaagac
     3781 atcaatttac aagtggaaaa aatcactcaa gattttgccc aatcactaac tgaacacaaa
     3841 tataaaggct tctttggttt agactttttt gtctctaata atcaggtcta tttaattgaa
     3901 tgcaatccca ggctgactgc ctcttttgcc ctctacacca ccatcgaaaa acaccatcaa
     3961 ttaacacctc ttttttttct tcatctggca gaatttatta atatccccac caacgaaaat
     4021 actcattttc actacgaatt atccggttca gagatagtca acaagaaact aaatagaaaa
     4081 tatcaaagtc ttcaagcctt ctcgccctcg gccaatccga ttgccatacc ttcagaaata
     4141 attgccaagg ttttatgaac tcacgcaaac ttctttatat agtcttatca ctccttacca
     4201 ttggtttggt atttatctct gtttcttcaa tctcagaagc cgaacacact gtcggcgaca
     4261 agctctattt catcaaaaaa caagccatct ggtctaccat cggcctcttt gtcttcttta
     4321 ttgccaccaa aattaaacta agtcacgtca agaagtatgc ttctttgttt tttgttcttt
     4381 gttctatact cctagctcta gttctaattc cccaattcag taccgaaact ctcggtgcca
     4441 gaagatggct ttatttagga ccattgggga tccaaccttc agagatactc aaactcgctg
     4501 ccattatatt tttttccaaa attttctctg aaacaaaatc tctcaacatt aagaacctcg
     4561 ctatctacct ttcactaccc ctcaccttag tcatacttga accaaatctc agcaccgccg
     4621 tcctaattgc cgctatcgtt attactctct actacattgc tggtggagaa attatttctc
     4681 tttttactct ctgtataatc ggtgctgcca tcagcactgg cttaattatg tcctcgccct
     4741 accgactagc tcgatttgac tcaagctcct atcattcaag tcaaatgact cttgccttaa
     4801 cttccggagg accaattggt aagggttttg ccaattcgga acaaaaatat agattcctac
     4861 ccaaaatttc tactgattca atctttgctg ttattggtga agaactggga tttattggca
     4921 gcagccttat cattctactt tatattcttt ttatcgccac cattatcaac ataagtcaat
     4981 caactaacga tgttttctct actctttttg ccgcaggagt agctagctgg attagttatc
     5041 agagcctgat aaatgtgtct gctgttgtat ctattattcc cttaaccgga gtccctctac
     5101 ccttaatttc ttacggcgga tcatctctcg ttactctcat gtttgcttta ggcttggtat
     5161 actcaattag tcgtgaaaac aataaagaaa atcatcatca ccggcaacca ccacaccccg
     5221 gccatagaac taatcaatct actaaaaaaa gatagccagt ttgaatggga aatccactat
     5281 tttggacata tctctcccca agaaactcat atctctcaca ccatcatccc caagctcaac
     5341 attccttttt ataaccttga ttctggtaaa tttaaccgca aagacattat tctcaccatc
     5401 gttagttttc ccaaaacgat ctattctttt tttcgctctc tttccctggt tagacaaata
     5461 aaaccagaca tggtcatctc ttttggagga tatgtttccg tccctgttat cattgcttca
     5521 ttttttttgc gcacaccttc tatcactcac gagcaaacca gaactactag tttatctacc
     5581 tatatcaata gtttttttgt caaatatgta gccctttcct ttgataaatc cgataaatct
     5641 aacaaagaaa tatttaccgg caacctcatt cgtcaggcta tttttgacca aaactcacct
     5701 aagtttaaaa attttaaatt aaaaattaaa aatttaccca taatctatgt cactggaggc
     5761 aaccagggtt cagaattcat taacaactta gttaccaaat tacttcctca gctacagaat
     5821 ttctccataa ttcatcagac cggcaaaaaa aacattccta acaatcaata ccccaactat
     5881 ttccactatg aatacattga aattgaagat atcggttggg ttctcaacca cgccgacata
     5941 atcattagtc gttctggggc taatatttgc caagaactag acattctcaa caaaaaatca
     6001 atattaattc cacttccttt tactcaacaa aatgaacagc tacaaaacgc tctttggtta
     6061 aaaaacaaaa aacccactac caccgttatt ctagaacaag acacattaaa accaaccaat
     6121 attattcaag ctatcaagga actacaacga cacaaaacaa agaaaaatgc tctcaccaac
     6181 aactctgcta cccaccccat tatcaaactc atccaccaac ttgtttaata aattaattta
     6241 tctcactcca gtattgatca ttttcctaaa aatattattt cttcgtaact acaatctcgg
     6301 accagaagcc accccactat ctattaaaga taatttttta aaggaaacca ctgctctact
     6361 ctacactgcc aacctcaatc cacttcaaat tactcaccgg gactatcaaa atgagctgga
     6421 attctatcta caaaactcat ctggcagggg atatccagtc attttttcca ccaccaaaga
     6481 ccctttaatt caagttaccg ccttacaaaa aattatcaaa atcgctaata taaaagaaca
     6541 agagattaat tttattgatt taagcgccca aagaccttat gcaacactct aaaataattt
     6601 gcgccatcga cattgggaca tcaaaagtaa ctaccatcgt tggtcaacac tttgaaatcg
     6661 aagaccgatt taatgtcgtc gccgtttctt caattcctgc cctaggcttc aagaaaggac
     6721 agataatcaa ccttgatcaa gctactcaaa caatcaccca aagcttagaa tcagccgaaa
     6781 gaatggctgg ttttcaaata aagaatgccg cagtctctat ctgctcacca cacatagaat
     6841 ctattaattc acatggagtc gtagccatca gcactccaaa cggagaaatt actcagacag
     6901 atattaaccg agttatggaa gcttccgagg ccatttctct tcccgcaggt aaagaaataa
     6961 tccactccat cccccgacga tattctgtcg atggacaaga gggtgtagtc gaccctatca
     7021 gcatgaatgg cattcgtctc gaactagagt cacaccttat ccttgccagt agtcccagcc
     7081 tcaaaaattt acaaaagtgt ctcgatgatg ttggcataaa agtagaatca ctagtttatt
     7141 ctggactggc cactgcccag gccgccttaa ctaataccga aaaagagctt ggtgtcgctg
     7201 tcgtcgatat tggcggaagc gttacctctc taaccatata taccgaaggt tcaccagtat
     7261 tttcaagcgt acttcctatt ggtggcaaca gcgttaccaa cgatatggcc ataggcttac
     7321 gcctatctct cgaagatgca gacaaagtca aattaaagtt aaccaaggtc accgaatcca
     7381 agaaatacga agacgagatc gatctttcac aatttggagt cttagctgaa gaaaagcaca
     7441 aaatatctct acaaactgct atcaacggta ttatcaaacc cagactagag gagatattct
     7501 ccctagtttt cgaccaaatc gaaaacagcg gattttccag ccttattcca gctggagttg
     7561 tcctaactgg cgggggagcc atgactatta atgccaaaga tatttgtagt aaaatcattc
     7621 ctctcccaat cagagttgcc agccctccaa agctaggtgg cattgtcgat gatattctca
     7681 atccaagctt cactagcgtc attggcctct tgatgtataa tctggaacat agtaaaaacg
     7741 gatccaacac caaaaaatca aaggcttcct ttgaaggttt atttggaaaa attaaaaatc
     7801 tcctcgaacc actactgcca taattatttt ataaatactt taaattcgta attcgtaatt
     7861 tgaaattcgt aattaaaaca aatgggatta ataaaaaact taactggaca gttcgctagg
     7921 ctaaaagtaa tcggcgttgg tggtggcggt aataacgcgg ttaactccat gatcaccgag
     7981 gacaatatta aaggtgtcga ttttattgcc atcaacactg actctcaagc acttctcaat
     8041 tcactcgctc caatcaaaat ccaaattggt gaaaaactta ccaaaggcct cggagctggt
     8101 ggcaatcccc aagtcggagc cgaagctgcc cgagaatcag ccgaaaggat aaaagaagcc
     8161 ctcgagggca cagatatggt ctttattacg gggggtatgg gtggtggaac atgtaccggc
     8221 gctgctccag ttattgctga aattgccaaa aaagaaatag gtgccctaac tatcgccgtc
     8281 gtcaccaaac cattcctctt tgaaggtact cgtcgtatga ctaatgctga agaaggcatt
     8341 tcccaactca aagataatgt agacaccctg atcgtcattc ccaaccaaaa ggtcatggag
     8401 atagtcaacg acaaagccac cctaatcgaa gctttcaaaa tcgccgactc agttctcgat
     8461 aaaggtacca aggccatcgc cgaccttatc actactccag gattaattaa tcttgatttt
     8521 gccgatataa aaaccgttat gagcaatgcc ggctcagctc tcatgggagt aggggaagca
     8581 gaaggagaag gtaaagctca aaaagctgtc caagctgcta ttgattcacc tctagtcgaa
     8641 atcaatattg atggtgctcg cggagtactt atcaatatta ctggtgggcc tgacctgacc
     8701 atgtctgaga tcgaagatgc cgccaaaacc attactgatc tatgtgctcc tgatgccaac
     8761 ataatttttg gtgccacaat tgacaaggaa ctttcgggta aaattaaagt tagtgtcatt
     8821 gccaccggtt ttgacccgaa tcgtcaacga ttaatgcaat ctagtagaaa acaaaacgta
     8881 accacctcag aaccggtaac tagcaaagac tttcaagata tacttggtga taaggaaatc
     8941 cccatcggcc tcgatatcag tgacgaactc gacatccccg ccttcctccg caagaataaa
     9001 tagattaata tataaaataa ttatgaccat cagacaacca caagaaatac ttggactaga
     9061 ttatcaaaaa attttagaac cgacagttga cgaaaaactt tcacaaatta tagagatcat
     9121 gcaaaatgag gcaagtaggc taaaagcagt ttacacaaaa acatcaaaac caatcatcga
     9181 tgaagcaaca gcaatattaa aaccagatga aagagaagta tttaacggaa ctttaaatac
     9241 atatattaat ataagatcaa agactagcgg attacacctc caaaatgcct ggaatcataa
     9301 tttagaaggt caaacagaag tcgcaagaca agatttagaa acattcacta acgttcttga
     9361 atggttagag ccagagttga gtgacccaca acaagttttg catggattag aacaaagaat
     9421 acaagaagaa acacaaaggg cgacagccac tcaaggacaa ctaacacatc caacatccac
     9481 taaatcagat aaagaagttg aactaggtaa aatactcaac ataacccaac gagtgatcgg
     9541 caatttaccc caaacaacaa actgagacta tcttgtatcg agatgtttaa agaaattcgc
     9601 gaggggtagg tgtacatatg tgcgtgtggg cggccttttg ggccacccac cacttccccc
     9661 tcgcacatcg tactattctt ttttttctat aactgtcacc gtcaacggaa tcgacagtgt
     9721 ttctgcagga tgataatcaa tcccaatttc aacaacaaca tttatttgat atgtgcccgg
     9781 ctccaacgaa actggagcat atatcccaaa ttttttagag ccagtacctg ccccatacaa
     9841 aagtccatcg ctacaaaccg aaatcccgtt cataggatta ttcgagttca tgggcaaaaa
     9901 attgaatttc aggtcgtcaa cgaacgcttc cagctccaca acaggtgacg aacactctcc
     9961 ctgagcaact tcgatttcag tcaatgaaag agataacttt cctctttcac caaccatctc
    10021 aggcagtttt ggggtcagta tgggagtggg ctcaattgtc ggtgcaaccg tcagctcaac
    10081 agcaggaata acgggggact cggcaggacc gcaagccgaa agggccaaca acaacaaaac
    10141 aatcaacaac tttttcattt ccgtttctcc ttggcactta attgtgccgg ctatataaga
    10201 tcaaaattga tcctatgttt accggcacaa tttattaaaa aaatagcatg atgattttaa
    10261 agaactgtac ctaa
//
LOCUS       DDWP01000063            9244 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_23285, whole genome shotgun sequence.
ACCESSION   DDWP01000063 DDWP01000000
VERSION     DDWP01000063.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_23285"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3377 a         1501 c         2127 g         2239 t
ORIGIN      
        1 tgatgaagat gttggccgtc ggacagaagc aatggccttc tttgataaaa acgtcgaagc
       61 attacaaaaa cagcgtcaat cataggtact gctagaagaa gagtaatagt ggcgagctta
      121 gcaccagaaa gtatcgacag gacagcgaga aaaagtccag ctaaactctt ggcactgtag
      181 ccaaccataa tgctttgagg atagaagttg aatggcagaa atcctaagta agccccggca
      241 acgctaccag ccaggataat aaccggccac tgggtagtat caccagcaaa tctaagcccc
      301 aatatgccca caaacaaggc agatatggag acaaatccag ggagctgacc ctctactcca
      361 ccagcccaac caatgacatt catacaccaa ataatccaaa cgatagcaag aatatctgaa
      421 atcacccaga gactatgtgg accaaagaat tcaaaactaa aacgaaaaga agaaagatcg
      481 atgactccac caaacgggtt tgagatatag gcaatgccga tacctgagcc aactataatt
      541 agccccgaga tagtgttagt cagaagtcta accctaggac taatgtcttt aatatcgtcc
      601 catagtccta taactaaagt aagaagggcg ctagtgaata tggctaacag atgtttatct
      661 aaaggtagaa aaatagaggc agaaacaacg atagcaataa aaattggcag gcctccccct
      721 ctgggaactg agaatgcggt ggtggcatta tgagtcttgc gttgctttaa cgcagggtct
      781 tctacccaat tgtgactaat aaaaaagtct ctaataaatg gagtaagtag gtaagaaatc
      841 agaaatgcca aaaagatgct ggagataaat tggataatca tcctaagaga taaggctaat
      901 aatttgataa gtagcgatgg tggaaaagaa ggaaaaagcg agagatccga caataagaag
      961 acgagacaaa agttggactg agttcagaaa gaaaacagct aaaatattgt tgataattaa
     1021 gaccagcaca gaagctacag gaaggatgat aatactagag gcagaagcaa gctggacctc
     1081 accccaaggc tgtgaatagt agagaggtat ttgaggtggc aggctactga acttcataag
     1141 aatcataccg aattgaacta aaacaaaaaa aagtgtccaa cgaaaaaaga ggctgtttat
     1201 cttttcctgc caaaaaagac cgatgcggga gacggcaaaa gaaagtggct taaataagtt
     1261 cattggtcca agtcaacctt gttaaagaca accacttcac cgtaagcaat tgaggggaca
     1321 tagtagcgag agataaaaag atcaagttca ggaaaaggtc taccgggctg aggaaaataa
     1381 acaatgaact tgggataagc agtcctaaga tagtccatgg tggcagaatg ctgtttgaag
     1441 tcagtaatgt ggtaagcaac tgtatatcta ccgacaggta atcgctctga caaggcgtaa
     1501 agatggggat tgtctcccca aacaaaaact ggttgatcgg ggctcgagtt gtctttaatg
     1561 aatttagata tctggtaatc ccgctctaaa gattgcccgt agtaggacat ttggttaccc
     1621 tgtagataga aattacgata ataagaaaaa acaggataga cataaaactt atacttgaaa
     1681 aaacaaaaca caagaaacaa aatacagaac aaggtgaaga gacgaatttt tttggacaat
     1741 tttgaatcaa acaaaaagat gaggaggaga atgagtggtg gcaaaatttg gataagatag
     1801 tgaggatatg gacgagtgga cagaagagcg ccaaagatgg tggcggcaaa ccacaaagtc
     1861 aagaaagaaa ctttctcaga aataagtttt ttgcggtaca gaaccaatga taagagatag
     1921 aaaacagcaa ggatagcagc ccgaccaaga agtccactat cagttgaaga accagagtgt
     1981 gagccagtag accaggaaga taggtatcca aaattctgaa acagggcggc aaagaggaat
     2041 tcattaactg cacctttaaa ataaaaatag accaaatata gaagtgtggg gagtgagaat
     2101 gagataagaa gtatgaagta tgatttaaga ataaaaccaa tctttttaag gtcaaaattg
     2161 gtgattaata tccagaaaca aagaaaggca aattcaaaag cgacgggaat cttgatagta
     2221 aaggcaaaac caagaaggag tcccgccgcc gccaaggctc tggcgggcaa gcctgatttt
     2281 ttgacagaag agatgagata aattgcccaa attgtgggaa ggagcataaa gatttcagca
     2341 ttagctatag atccttcgaa aagaggaatg gaagtgagga tgataaagaa caaagttgca
     2401 agtcgggaaa atcttaaagt caaaaatcta tttgataaga gataaaatac gtaaacagtg
     2461 gggatcatcc ataatgcaag tagaagtcta aaaccaaaaa ctgtctggcc aaaagcagcg
     2521 agatagtaga gagtaggagg tttgttgtcg tgaatttgac tataaagagt cagccctcgt
     2581 tggatggcct gacctagaac tagatagata ttctcatcac cataccagta tggttcaaag
     2641 agagaaggga gacggagaaa taagaataat aaagaagcaa aaacccaaaa tttatttttc
     2701 ttgatccaag taaggaacat tgatttattt tagcactaat atttagatgt catcgcgaga
     2761 caggcaacaa catgagaatc aactggcctt tttttgagtg gttttgtatt ccaggcgatg
     2821 accaaacatt aatcgagtgt gtgaatgaag cccaggtaaa actgacatgg ttaaagtagc
     2881 tagtggcatg aaaggccatt ggaggatatt tttaagaatg tttctaaaac ctgtcttctg
     2941 atatttggga cgtaatttcc aatcaagaac tattaaaaca gtaagtggaa tgagacacag
     3001 ggtgagaatg acgttggata ttcgaggtaa gttgaaacca atagctgtct ggaaaaaagt
     3061 cgggttaagg acgacaggaa gagaagtgcc aagagtaaga ataaaccaat tactagacca
     3121 aataaagtga gtctcaatta atttgaaaat acgaagaacg cgagttatga cggggatctc
     3181 tggatgacgc atagattgag aaatggcata aggaatgtcg atcgcacccc aagcatgacg
     3241 taaacactga ttgtatcgat tcttgagagc gccaaaataa ctctgcccgt ctggagcatc
     3301 aactacagta gggagaaata ttggttcaac acggcttcta cctcctgaag caaaaaaagt
     3361 ctggaggaat atatgccaat cttcgggaat catgtcgatg tcccaatagc cagcttggtc
     3421 aatcaaaaga taagaagagg aataacaaga ataattaaag aacagaccat caggctcttg
     3481 aatattagca atgtgaataa cattgcccaa agtaccgata atacgaatcg gagcaggaac
     3541 actgttgata ttgtgatgcc aaaatatggg tgactgccaa aaaagataat gacgactaga
     3601 cgaactggca aagctgtagg ttagagcaga gaagtatttg gggtgaaaaa gggtatcgac
     3661 gtcacatgaa gttaaagtac aattttcaat ttttagtttt gtaccgagaa acttcttctt
     3721 aaaatatttg gcagcataag cctcattgct atgttttcca acagtttcgc ctggagtatc
     3781 gctagggtgt tcggtaaaaa ttaggttacc aaagatgttt ttgtattttt tgagaaagta
     3841 atctttagta gtattgttgt tatcttgacc agccctttgt tcttgagcca agataacgtg
     3901 aattttttta agattgatat caatttgttg agatagactg ccgatagcca tatcgataac
     3961 ttcaacaggc tctttatagg aggaaatgac gaccacgtga ttaatatcgt tatatttgaa
     4021 ccaatcagcc ctgaagtttt cctgaaacag gtctagccaa tttgtcttac tagcagtaag
     4081 tattttaaaa taaccgataa cagcgaaaaa agctgacttg aatgactggt aaagccaata
     4141 aatcaaaaaa gcgataacaa agtaggcaac tgctttagga aaaaaaatgg ctccccaaat
     4201 aggaaaacta atcgtagtcc aggcgaatat cccagggatc atttccaaga gacgatagga
     4261 cttggtattt ttattcatat aaagactatt tattatatca atcaaggtag aataagtaat
     4321 gagtcggtct cttaggcaag caattttgaa aaccataaga tatggacgtc ggtttggggt
     4381 tgaatataca gagcgagaag tgatggagag attgatatcg gataaaatat ttagtctaaa
     4441 agtcaaaagt caaaagtcta aaagtcagac tgttgaaaat aagatgaaac tagcaataag
     4501 actggcaaaa gagattgggg gcagatttgg cgatatatta tttattgggg taacaggatc
     4561 agtggcagca gggtatccac cagagaatag tgatatagat ctaatggtag tgacgagaaa
     4621 aaatacttta tggttaacaa gattgctgct aagattttgg gtgtggcaaa aaggtatccc
     4681 ccatcgaaaa tatggcaaac cagaacttga gaatgagttt tgcttcaact tgtggctaga
     4741 agaagactca ctaaaattac caaagaacag acaaaacttg aagaatgcaa tggacttgat
     4801 attaatgaaa acgatactaa atagaaataa tacttatgaa aaactggtga tggagaatga
     4861 ttgggcgaga ttcttcgtag ccaatggcta cgaaagaata aagtcaaaag tcaaaagttc
     4921 ccccaagggg acttccttat gggcgcaaaa gtcaaaagaa agcagttggt taaagaaaat
     4981 aataaattgg acggtgtttt ggccacaatg gtggtacatg aaaagaaaag ttgggagagg
     5041 aataattgat aggaaaaggg ctttttttca tccgggcaat aagtgataca ataaatgtac
     5101 tcatgatttt gaatcactta ggaaaggcag gtattttact ctttggattg ttgatattgg
     5161 tagtgtctct ggctagggct ggtttgacga ttgcgcagga aaataccgaa gaaataaaaa
     5221 aaaatgaagt atggtttgta atgactgata aatttggtaa tcgtgaatca aagtcctaca
     5281 agctgccaga ggtgagagta ttaccaagtt ctatgtttta tggctttaaa agaattaggg
     5341 attatttgtg gttgtcattt agctctggga tggataaacc aaaaattgcc atattaatgg
     5401 cagataagaa agttactgaa taccaaaagc tggtagatga aggtaacaat cagttagcag
     5461 ttgaggctgg tattgaagct gtagataaat taaaatacgt tgacgaattg atatcaacaa
     5521 taaaccctca aaatgatcaa acaagagaaa tccatcaaca agttttacga ggagggttgg
     5581 catacaaaga aatatttgtc cacgaaaagg ccaactttga gctggtggag ggaggatatt
     5641 ctaatttaat ttcaacaata gatgagtgga acaaagaaca agaagaccga cgttttgaat
     5701 gggatcgtta gtaaggggaa attgtggttg gtacttggtg tatttataat cacgctagtt
     5761 agcatagctt acgtactcag ggatatacca aatccaaaac agttatcctc tgacatatac
     5821 ccagaatcat cgcaagtgct cgatagaaaa ggtagattga tatacgaaat atacgcagat
     5881 aaaaggcgaa ctccaactaa gttagaggaa ataccagatt cactaaagaa agcaaccctg
     5941 gcgatagagg atgctaactt ctatcgacat aatggttttg atgtaagagg aataatgcga
     6001 ggtttattcc ggacaatagt gcaaaaaaga cttcaaggag ggtctacact aactcagcag
     6061 ttggtgaaaa atgcactact aactccagaa aggacttgga ccaggaaatt taaagaggca
     6121 gtgttgacgg tggctaccga agtgatgtat accaaagacg aaatattaga aatgtatttc
     6181 aaccaaactc cctacggagg aacaatgtgg ggtgtgggag cagcagcaac aggaattttt
     6241 gacaaagaag ctagtgaatt aaccttggct gagtcggcaa tgctagctgg tttaccagga
     6301 tcaccaacaa gatactcccc ttttgcctat cctgaggcag ccaagaacag acaaataatg
     6361 gtacttttcc gaatggagga acttggtttc ataaccagtg aagaacgaat ccaagcagaa
     6421 aaagaagagt tggtttacaa cattaagaaa agcagtatct tggctccaca ttttgttttt
     6481 tacgtaaagg agctattgac agaaaaatac gggactaaag tagtaaatga gggtgggctg
     6541 aaagtaacca caacattaga cctagatata caagactatg tgcaagcagc agtggcgacc
     6601 gaagtggcaa agttacagag atctaacgta actaatggtg cggctttagt tactgaacca
     6661 aagacagggc aaatattagc aatggtcggg tcaaaagatt tttttgcaac cgatatagag
     6721 ggtaaattta atgtaactac ggcgctaaga caacctggat catcgattaa accgataaac
     6781 tatgcggtag gattagaatt aggtaaagtg acagcagctt cagtttttta tgatggacca
     6841 acgtgtttta aagtagaaaa ccaaaaacaa tattgtccga caaattatgg tggcgcatat
     6901 cacggaatac agacactcag aaactcacta gctaattctt tgaacattgc ggcagttaaa
     6961 atgatagaga tgaatgggct ggagactttt gtagcctcag catcggccat gggtattagt
     7021 acttataaag atgcttctca atacggattg tcaataacac ttggtggagg agaagtaatg
     7081 atgacagata tgaacgtagc gtttggggta tttgccaaca tgggaacgag gcaagattta
     7141 acaccgatat tgaaagtggt ggacaaaaaa ggtaacacta tagacgagta taaatatatt
     7201 cctggagaca gagtcttgtc gagggaaaca agctatataa ttggcgacat actctccgac
     7261 gatggagcac gaagcatggt gtttggaaga gggtcaatgt taaacatcaa gggacatttt
     7321 gtagctgtca aaactggaac caccaatgat ttacgtgata actggacagt gggatatacc
     7381 cctgattatg tagtgacaac ttgggttggt aataacaata ataccaagat ggctggagta
     7441 gtctctggaa ctagtggagc tgcacctgta tggaacaagg tgattagtaa attacttgaa
     7501 gaaaaaaata caaaaaagcc agcagttccc gctagtatag taatgatgag tgtgtgcaac
     7561 ctgactggag aactgcctcc tgagggtggt tgtgaatctc ataatgaata tttcaaaagc
     7621 gagtttgcac caaaaaacag agccccacta agacagtcag tactcataga caaggatact
     7681 ggccaaatag ctgatccagc taaaaactta ccaaatgtag aatggcaaga taaagctgcc
     7741 ataagagatg taagcggtag gatggcatgt ttagagtgtc cacaaattaa cgaaaataac
     7801 taatgtctgg acttttaata ataaaaaatt ttttaacgac attaggaaag ccagtctata
     7861 ggactatagc tgtgacgttg aggttggtaa gaactattgt cgcaagcctg gttactacac
     7921 tcagaaaaat tcaatgggga tacctcctcc caagcctggt gctgacagtg atgtctgtct
     7981 ttatgatatg gctatatata ggggtactgc gtgacttgcc ggacgtaaat gagatatata
     8041 acccgccgag attgtcaacc aaaattcttg atagaaacgg acacttactc tataaatttt
     8101 acgaagatga gaatagaaca tgggtaagtt tggagcaagt accagcttca ttgatatggg
     8161 caacattagc aattgaggac aaagattttt atagccatca cgggatatct atccgaggta
     8221 tagctaaagc gataatttat aacatattca aaaaaggaga agatggcaat gttagaggag
     8281 ggtcgacaat aacccaacag ctggtaaaaa aagtattttt tacgggtgaa aaaacttaca
     8341 ccagaaaaat aaaagaggcg gtagtgtcga tgtggataga gaccaaattg agtaaagacg
     8401 aaatactgga aagatatttt aaccaagtac catacggagg tgacacatac ggagtacaag
     8461 aggcatcttg gagatatttt gggaaaaatg tatgggaact gacgatggca gaagcatctt
     8521 ttctggcagg actaccggcg gctcccggct cctattcccc ttttggtaat aatccagaat
     8581 atgcctatgc aaggcagaag tatgtggtca aagagatggt aagtgcaaaa tatataactc
     8641 aagaaaaagc gaatgagttg ttagccaaca gggtagaggt cggagcccaa cagagaaata
     8701 ttctggcacc gcattttgtc ttttatgtca aagatttttt acaaaaaaag tttggctata
     8761 ctgactttga aagaaggggc ctgacagtaa aaaccagttt agacataaat gtacaaagac
     8821 tggcagagaa agtagttgaa gaagagataa aagcagtttc tcgactaaaa ataggtaatg
     8881 gtgcggcgat ggtggtcgaa ccaaagactg gagaaattat cgcaatggtt ggctcaaaag
     8941 attactatgc ttcagatatc gatggcaagt ttaatgtaac tacagcacaa agacagcccg
     9001 ggtcatcaat aaaaccactc aactatctac tagcactgac aaaggggtgg tcattagcat
     9061 catcaattga cgactctcca gtggtatatc aaataaaagg ccaggccaaa ccatatatac
     9121 cccaaaacta taatggtaaa tatatggggc aagtaaatct gaggacagca ctagcatcta
     9181 gtctaaatat accggcagta aagttactag cacataatgg catagataac atgattgata
     9241 tggc
//
LOCUS       DDWP01000064            7183 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_39283, whole genome shotgun sequence.
ACCESSION   DDWP01000064 DDWP01000000
VERSION     DDWP01000064.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_39283"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2307 a         1857 c          858 g         2161 t
ORIGIN      
        1 ttttattttt ttccctcctc cccccctccc tctcccaggt tcaatagtcg caaaagatac
       61 attgtgcgac tatgtataat ccacctcccc tctggtagag gactaataac tattaataag
      121 caatcatccg gcattaaaag gtcaaatata attaaaaatt gtaaattata aattgtaaat
      181 tgactctatc cactcctctt atccctactc caggcataca gtctgatata cttatcgatt
      241 ccccagtttt ccacacagac tatatcattg tgtatcatca ttgcgtgaaa attataggat
      301 ataatgtgaa aaattaatta atattatagg actaaatatt ataggctact ctcctcccct
      361 gtttctggca tggcaataaa atttttttgt gccaattcgt taatttagct tcaaatatat
      421 tgtttttagg ttcagagctg gctccattta gctgtctttt catcatgaat tcagatttac
      481 ccaagcccga ccactcaaga aaacccactc tcgcccctcc cctaccactt ttacgcacaa
      541 aagtacatat tatacctaaa aacaggcata atactattta ccacctctag ctaatagatc
      601 taatttatta ttagataatt cagaacctag tagcccttta acttttccca actcttccgc
      661 ccccaaactg cctccccgct ccataaatct aatagatgcc tccttaacca agtttccaaa
      721 gactttactg cgtcgtctat tatcgtacaa atcagttaaa agacccaatg tcttttcttt
      781 gttttgtata aaagcccaag aaattattgg taaaactgcg tgaactgctt ccgcagaata
      841 gtcgatctga ctcattaaat attcttctaa cattccaaac actgcatctg gaaatttttc
      901 tgtatgtatc caattcggat caaaatattt ttccaattta ccttctaact cctttggagt
      961 gaatccacat ccaccccaaa aggtatcgtt accgcctgtt tttaccgctc cgttaatatt
     1021 tcccaacaaa tccccgatta ctaaaccata atctgcataa ttttcaccac tcaacatata
     1081 actcaatctt gctggattca aagccaccaa tccaaaactt ctttccaaat aacctttatt
     1141 aaagccacgc ttaagtgcct caaagttgcc actaccatat ttatcaaatt catcgtaacc
     1201 tacaccagaa aaccattcag cgatattgta atgcactatt cttccatcat aatcagttac
     1261 actcttcacc ggatccaatg gatgattcga aaagtcatgg agtacaattg gatctccatc
     1321 tagttttcct gtagcatcaa agtgagcttc tatcattacc acattggtat ggtcccccgt
     1381 aggtagcaat tttttatcta aatctgggat aatcattgcc gattctggtt ttaaacgtcg
     1441 atatgaacaa tataatcgtc gttgcgaccc atctttttcg taacgcacag caacaaatct
     1501 atttgtgtat aaagtatcca cacccataac cttgtattta gtatccaatg catcaggcga
     1561 tgtatacaca tatgaattgt aattagctgc cattaccccg tcctcaccgc taccaaccgc
     1621 ccccagcata tcgacatctt cagaaccata ccatttaata tgagccattt gattcaacat
     1681 atctttagca atagggctta attcaaagtc ttcaaaaggt ttacggacca cttcaaacag
     1741 attttcctgt gcttgtattg ctaataaatc tcgtcccaat aaagtttttt catttatgcc
     1801 tgctctttca attgtaagtc ccatgttttt aattattaat tattaattat taattttcta
     1861 cccccacttc gttatcatca atatagccaa agatattgcc accgccaccc atgaatacat
     1921 caaccatacc gctttaaaca gacgatctcg gatatctgcc tgtatcaacc cactcatgat
     1981 ataaaccacc gacaccaagt aaacagaacc tttaaatatt cctactggcc agaaagaaaa
     2041 caatatcgcc aactgcgaca ttagaaccga aggaattatt atgtaagact tcatattttt
     2101 ttcagctcca tcgtcaggaa gctcaatagc aattgcccag aactggtaag caaacaccgc
     2161 caaagagatc aggaacaccc acagtccgtt ccaataataa gccaacctaa acgacaacaa
     2221 gctgtcaaac ataaaaaacg acgacagcaa caagactatc agcgaaactg tataagcggc
     2281 ccggtataga ggcactgttc taaagccaat tgccaccatg aatacatttt ccaccagaaa
     2341 cattacatac agcacaaatc caaatatcac gctcgaaatc aaccaccaca ccaacccata
     2401 tggcaacaaa gccacaaaca ttccaaaacc aataaaaaaa caaatcggca acaataccgt
     2461 cattatcctt agataaagac ttttctcaaa tatcactcca aggccaaacc aaaaacaaaa
     2521 aaccaccaag acaattccca ccatcagtcc aaaatacctc gactcatacg gcaaagagat
     2581 aaaaaagaaa aaccctaatg ccgaaaataa cgaagccacc atataacgaa atcttttatt
     2641 catgatagtc aaaacccgca aaaacattaa ccacatcatc attttcctcc aagcactcca
     2701 ccaattcgac aacatcattc aagtcacttt cgctatcaag caatattggt gactttaccc
     2761 tcattacgat accgctttcc tccaccacca aaccaatttt ttttatttct tcctccaccc
     2821 tcttcagatc aactacctca gtcaacacag aatttccatc aaaatcttta gcccccgcat
     2881 ctatcaaggt cagttgtacc gcttcatcta gttccgacac aacaatctcg ccgactctat
     2941 caaaaaagta tgccacactt ccttcttcac ccaaacttcc accagagttc ttaaatataa
     3001 gtcttatttc acctaagatt cggtttttat tatcagtttc aatctccacc atcaccggta
     3061 caccatgtgg cccaaagcct tctaaaatac aattaatcag attacctttt cttgattcaa
     3121 atctagctaa caaacggtca acattctcct ttggcatgtt taccgccttg gccttatcaa
     3181 tcgcatcctt caggccagat cctccaccat ttctcagcgc agtcaagata tcttttgagg
     3241 ctttagtaaa tgccacactc ctctttttat cctgagcccc cttcctgttc ttaatatttg
     3301 cccacttcga atgacctgac atttattttt attttttttc taaataactt tcgtagttta
     3361 tcattttgga gaacccatct ccaaagtata tctctacttc attggctccc aacaactcgt
     3421 caactgcctg tccacaaaac ggcaatacag ttttcgccag cttcactcca taccaattat
     3481 ctccactttt agaagagaca aaaagacatg ggtcatcaat tactttctca gccgaatcaa
     3541 tccctattac agtcaaacca gctccctcca atctccccga cataaatgac cccaagccat
     3601 ttccttgact cacattataa acatttaatc ttaaatcctc cctcaacacc aagctattag
     3661 acagatctct ttgtatcagt tttcccagct tttcatcatc ctctattaaa gtcttagtta
     3721 ctagctcctg cttagtcatt agaccaaaat ggttaaaaac atattttaga taattaatca
     3781 gccctaattt agaaattaca cttctatcac gccaccacac agtatccccc tttgtcacta
     3841 cattaggcac aaaaccaaaa ttataaaaaa atacatccgc caacaaatct gtttttttct
     3901 cttgttcaag tatgctcaat aatttatcac tccgatacca tcccagccca cccggtatcc
     3961 acaccggcac ctcaccctcc acctcaaaca cattaaccat ccctctctgt tttgaaacag
     4021 tcttcatcga caatccacta ctatcagccg ttgccaccat aaaatcacca ttttcagaat
     4081 cattaaaaaa taccaaaaat aaagccactg ccatcatcaa cccaaacaac aacgatacca
     4141 atttaaatcg tttcatattt ttctagcatc aattttatat caggcgccaa ctcagattta
     4201 aatacctgac cattaatttc tgtctggtaa gcatgcaaaa atggtctatc caaaccgacc
     4261 attaatccac catatgtttt atcacctagt aaaggccagc caaggtaact aaaatgcact
     4321 cttatctgat gtgttcttcc tgtctttagg tcaatatcca ctaaagagta cattttcccc
     4381 tcaatcatat atttctctac caaacgaaag atagtttgag ccctcttacc tgattcatcg
     4441 actcggaatc ttccaaaaga atattttgat ctggcaatag gcatatctac ttcaccagag
     4501 aatggcaaat taccacaaac catcgccata tatcttttct tcaccttcct ctccttaaat
     4561 tgacgtttta ggtcaattag actctcttct gtcttagcca caattactat tcccgaagtc
     4621 cccttgtcca gtcgatgaac aatcccctgc ctcggcaaat tattttctct attgttttta
     4681 acccaatctt ccaaactcaa ttgttctaaa ttttgagttt taagttttga tgtttgaatt
     4741 ttaagttttg aattttcgtt agtagtcacc attcctgccg gtttatccaa caccataaag
     4801 ttatcatcct catagattat cttgacttct tccatatata gattattacc cctacaaata
     4861 tcaaccaatc attaaaatta ttatatatat ttgtccaagg tattctccag tagtctctca
     4921 cataaccaag agtcattctc tcccacaagt tcaaagcccc accaattatg attagtagta
     4981 atggccagct cctctctttt ttgtaccacc acaacaaagc tacaaaaaat attgctacaa
     5041 atatccaatg gacctcgatt ccaaaactaa ttccgctatt cctgatcacg ggcacaatta
     5101 atacaggtag ttatctctgg tttaattgcc aacctttcag tatcaatcat ttttccacat
     5161 ttatcacaaa gcccatacct ccccagtttt atcttagtaa gagcttttct caactgcacc
     5221 aaatgttttt taacaaaatc agccttaacc tcagcttcaa atctaccaat ttgttcatca
     5281 gtgtcatttt ctaacgaatt gccaactgtc cttgtatcgt cagcaaaagg gtcgttcttt
     5341 tttattttct tttcagccct cttccactta actacttctt cctccaaaaa tctctttatt
     5401 ggctctagta tatttcttgg aaaagtaatc accgatttct gctggtcagt tttcatcatt
     5461 agctagcata accaatccaa ctccagaaat caaacttaat atcagataga taattatcac
     5521 aattggccca tctttaagcc accaagaaac aagtgccaac atcaggccca caagtaggtt
     5581 cacaaagaag aagacaaaac ctctcttgcc acttttgtac cacacaaaag cacggtacct
     5641 accagatatc tggatagcaa ccaccattgc tatgacaaaa acaactgcat gaaaccacaa
     5701 tctttctccg agcatcagca aagacaagat taaatatatt atccaagtta tcttctccaa
     5761 aaaaggccac aatttccatt ctttatcacg gcagttccac caagttatca atatcagacc
     5821 caccactgct ccaacataat taaacccagg gttactccag aaggaaaacc aattaatcaa
     5881 actctcattc cacacccccc agtttattag accatagctc aaccggccac tcaccaatgc
     5941 caccgttact gccaaccacg aataatctat cagttcattt tctttgtaat catcacgcaa
     6001 atttctccat accaaataca aaaacaatac tagtactacc aatttcaata tcccaaaaat
     6061 cataattcat tatacgtcaa cttaaatctg tatagttcgc tttgatccaa gtccaactga
     6121 tatacgtttc agcagatcca ctgacagatt aaccggacgc tacctaactt atcaagttta
     6181 tcgccatgtt attattaagt tgtaattaaa atttaaatat aaagtatgcc agaaacactc
     6241 acaccacaac cagatttaag tcaaagagta accgaatcaa tagctaggtt tatctctgaa
     6301 agaccaagtt tcggagaagg aattttcaat attacctgcc aaataatgca attgttagga
     6361 gccgaaccag aaaaatattt agttttatgg agagatcaac acccagatga atatgccgcc
     6421 cttcaccaca acatgttcga agcaatgaaa aatttccccc caaaactaaa agaatcgttg
     6481 acttcagagc aactaggatc acctcaaaaa tcataaccta ttataggtta agttaaatct
     6541 gtatggttag ctttgaccca agtccaactg atatcctctt caacaagtcc actgtcggat
     6601 taattgtcga gttttctaat tgagaaacaa tcgcttgagt agtcttagct tttttagcta
     6661 actcctgttg cgacatctta ttttccaaac gttttctaat tatttgtcga gatatttgat
     6721 actcggcatc tgccttttcc caaagctctt taaactcagg atctttcaag ctctgcttaa
     6781 aatacttttt aaaagttaac agtttcatag gttaattata tcgtatacaa tatatatgtc
     6841 gagtcgacac actaaggcga ctcaacacct ccttacacta tctctaccta agaatcttat
     6901 ttcccacaac ggcaaccctg ctaatttttt aagatgaggg atcactgaag ttaaaccaaa
     6961 ctcttcaatg ttattgaaaa ttacaaacgc tttgacttta gcatttttat tcttatctaa
     7021 aaaatccccc actgggctac gacccctctg atcaatataa aaattaaccc tagccatcag
     7081 taataatcat aacaaattgt gccgaatgag ggaatcgaac ccccatgaca ttgctgccac
     7141 acgattttga gtcgtgcgcg tctaccaatt ccgccaattc ggc
//
LOCUS       DDWP01000065            9022 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_18079, whole genome shotgun sequence.
ACCESSION   DDWP01000065 DDWP01000000
VERSION     DDWP01000065.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_18079"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2640 a         1779 c         1773 g         2830 t
ORIGIN      
        1 cgataaggca ttctcttggt taactgaaaa gcaattttct cggaaatcag cttggcagac
       61 aaatcagcta atttgaactc ttcaacttga atatcaatat tttgttttga agaatctttc
      121 ttgatgagtt tctgaatatc ctttttcaag gcttcgagct ctttacctcc cctaccaata
      181 acgagaccag ggcgagagac aactaaaatt agtttgagtt ttttggctga acgttcaata
      241 cgagtagtaa cgataccagc agaataaagt ttcttttgaa tgaagtcacg aatattcttg
      301 tcttccaata acaaaattgg gtatttttgt ttgttggcat accaagagga gagccattca
      361 ggactgtctt tgacagtctc aagtctgaag gcaattggat ttattttgtt tcccatagtt
      421 ttttaacttt gtattgtacc agataaaacg ataactagtc gactatgacg tttttgaata
      481 attccacggg caaatcgtga tccgtgtgat ttgtccatgc gcttaatctt cattccctca
      541 tcgactctaa attcggtgaa ttttaaggaa gcaggtaata ggttatagtt gttcttagca
      601 gaagcaacgg catcagaaat acacttaacg agaagacgtc cagcattagt ctggctaaat
      661 ttgagacgag taatacaggt aagtggatcg agcttcttaa tgtcagtgac gagaagtcgt
      721 agcttacgag gagagatctt aagatttttg ttgatgtatt tggcctgacc aagcttggtc
      781 tcgacagtct caactgcagg tttagcaacc tttatctctt taacaacttt aactttttcg
      841 atttttgatt taactgtctt tatttgagcc atatgttttt aggtcttttc aataacacgt
      901 ttaacgatac gtccatgacc cttgaatgtg cgggtaggag cgaactcacc taaatagtga
      961 ccaaccatat cttcggtgac aaaaacgttg ataaactttt taccattatg aacagaaaag
     1021 tttttgccga caaaatcagg agagatctgg ctcttacggg cccaggtctt aatggcttca
     1081 gtcgatgacg agttagcaac ttttttgact aagttggcgt caatgtatgg tccttttttt
     1141 gatgagcgtg acatgagtta atttttaatt taaaattttt aattttaaat tatttctttc
     1201 tcctttgaac gattaattta ttggatttgt ttttacgtga acgagtacgc ttacccatgg
     1261 ctggcttgcc ccacggagtc tttggattca taccaataga tgacttacct tctcctcctc
     1321 catgtggatg agaatgaggg ttctgagcaa cacctcgaac tgatggtcga ataccacggt
     1381 gtcgtgattg accagctttg gttaatttaa cgttaaagtg ttcagcattg ccaacttggc
     1441 caacagtagc ccaagcatct ccaggaaaaa ttcgaagctc gcttgaaggc atcttgacga
     1501 taactttgtt gccgtcaatg ctttgaatat aagcagagga accagcagat ttaactactt
     1561 gggctccttt accagcttga aattcgagac agtgaatagg gacaccaact ggaatttctt
     1621 taagaggaag acgattacca tctttaatag gagcggtcgg tgaagcaact atagtgtcat
     1681 ctactttaag attagtggta gctaaaatat agcttttgac accatcttcg tagacaacta
     1741 aggcaatatt tgcagtacgg tttgggtcgt attcgatggt agcaactcga gctttaacgc
     1801 caacttttgt cctcttccag tcaatggtac gaaggaatct tttctcttca ccacctcgat
     1861 gacggacaga aatgtgccct ttagcatcgc gtccactggt tttctttttg atagtaaata
     1921 acttcttcat gctatttggt gttgaaattg agtaattcta ttttttgatc tttggctaaa
     1981 gtaatatgag ccttcttacg ggtcggtttt tgaatgggca ttctcttgcg agcgtccatc
     2041 ttaactttac caagagacat tgaagtcctg atagaaagag gcttaatgcc aaagacggaa
     2101 agaaaggctt gatagatttg tgattttgaa gaacgtggac taacccaaaa agaataaaca
     2161 cttcgcgatt gagcggagat ggtcttctcg gttaccattg gtcctaagat gatttgttct
     2221 gggttgatag tagtcatgtt atttaagtgt tttaagggct tcctggctaa agactaaaaa
     2281 gttctgttta cctaagtcta ggacattaag agagttgaca gaaattaggc tcatgcctgg
     2341 aatatttcta aaagcccgca taacgttaga attttcagcc tcggttatga taccaatttt
     2401 tttgcttttt tccagtattt gactttgttt ttctaaaaga tcaaaaaatt tgaaagcaac
     2461 ttttgtcttg gggtcgattg atttgaaatc ttcgatagca ataatctgat tatttttagc
     2521 gaattttgtc aaaatagaat agagagcaat ctttttggct ttttgattaa tttttttagt
     2581 aaagttttca ttaccttgtg gaccatgaac actacctcct ccgacgaaga taccgacttt
     2641 ggaagtactg tgacgagcac gaccagtacc tttctgagca tacattttct tagtagtacc
     2701 agaaacttca ccacgatctt tgaccttagc atgagcattg cgacgatttg ataaataggc
     2761 acgaacactc tgagcaataa gatcgttgtt aactttggcg tcaaaaattg atttgggtag
     2821 ctcaatatct ccgactttct ccccttttag attgtaaagt ggagcagaaa gctgactgac
     2881 agttttcttg gtgcttacta caggtgactt agttgttttt gtcatatact ttaggctttg
     2941 cagatagtaa cccaggaatt tagatgaccg ggaactgaac cactaattaa tatttcattc
     3001 ttttctgtgt tgatagcaac tacttgtagg ttgccgatgt gtttgacttt attaccgtaa
     3061 tgaccaggca ttttcttacc tttgataact ttacctggag tctgagcacc aatagaacca
     3121 ggagcacgga cacggtcaga ttgaccacca gatactggct gacgtcggaa accataacgt
     3181 ttgatgacac cagcaaaacc atgacctttg gagacaccag taacattgac tgtctcacca
     3241 acggttagaa cagaatcaac agtaatttga gtaccaactt caggagaagt ttcagcaact
     3301 aactcgaatt ctttgaattt ttggggagtg atatctaatt taagtttttt caatttacca
     3361 gaggtagatt gatcaagacg cttacgatta ccgtaagcta cctgaacagc aacatagcca
     3421 tcaatttctt tagtcttgat ctgagttaca gtcaaaggag aaacgatgca agaagtgaca
     3481 gcaatacgct tgccttctgg ggtgtatatg ttagtcattg agcctttttt ggctataaat
     3541 ccagaaatca tagtttacat tttaatctct atttcgacac cagaaggtaa gtcgaggtgt
     3601 tgaagactgt caacagtgcg aagactagta ttggaaatat cgataagacg cttgtgggtg
     3661 agcacttgaa agtgatcacg agcattcttg tcggtatgag gactagttaa aatagtaata
     3721 agttttttat tggtgggaag aggaatagga ccggaaactg tagcaccagc ggtaacggca
     3781 gcgtctacga ttttattggc ggcctgatcg ataagtcgat ggtcgaaaga ttttagtctg
     3841 attctgattc ggttaccttt ggtcatattg tttaaagagc gatttattaa tgtaagggcg
     3901 gacacatcgg tccgccctta cggaaatgct ttatttgata atttcggtga tagcaccggc
     3961 gccgacagta aggccacctt cgcggatagc gaagcggaaa cctttctcca tagcgactgg
     4021 tttaattaat ttaataacga cattggcatt gtcacctggc ataaccattt ccataccttc
     4081 tggaagggtc atgtcaccag ttacatcagt agttctgatg aaaacttggg gacggtaacc
     4141 agagaagatt ggagtatgac ggccaccttc ttctttttta aggagaagaa cttcagcctt
     4201 gaactcggtg tgaggagtaa tagttccagt tttagcaaga acttgtccac gttcaatatc
     4261 atctttttca actccgcgga gaagaatacc gacattgtca cctgcttgac cttgatcaag
     4321 agatttgtgg aacatttcca caccagtaac aacagctttc ttggtcgcac gaagaccaac
     4381 gatttcgatt tcatcgttga ctttgacgat accgcgttca atacgaccag tagcaacagt
     4441 tccacgacct ttaatggaga aaacgtcctc caaaggcatt aagaaaggtt gatcaagttc
     4501 gcgcttaggc tcagtaatga aagtgtcgag agcttccatc aatttcttga tagattcgac
     4561 accatcagct tcaccattta gggctttaag agcagaacca cggacgattg gagtggtatc
     4621 acctgggtaa ccgtatttgg ttaacaaatc acggatctcc atttcaacca aatccaagaa
     4681 ttctggatca gtgacgagat cgcatttatt taagaagact acaagagcag gaacgttaac
     4741 ttgcttggca agtaggacgt gctcacgagt ttgtggcatt ggaccatctg gagcagaaac
     4801 gacgagaata gcaccgtcca tttgagcggc accggtaatc atgttcttca cataatcagc
     4861 atgtcctgga cagtcaatat gagcataatg gcgagcatca gtctcgtatt caatgtggga
     4921 aatagcgata gtaacgatct ttgaagaatc acggacagta ccaccttttg caatatctgc
     4981 ataagatttg gcactacctt gaacttttga gatagcggca gttaaagtgg tcttaccatg
     5041 atcaacgtga ccaatggtgc caacattaac gtggggttta gttctttgat aagtagctgc
     5101 ggccattttt tttatttact cctctttcgg aaatatttaa catttaatta tagctaaaaa
     5161 cgctttaaga ttgtatcagt aagattaagg ctatgcaata gagaaaatga agagaatatt
     5221 aaacaagatg caaaaaacat gattcaagaa taaaatttta aacttacaac atgccccctt
     5281 ctttgttgac agttacaact tcgtaatcac tcttaaattt atccttaatc ccttctggag
     5341 tcataaaaat aatttttctt ttgaagtatg gatgatcatc agcgattaac ttgtcaattt
     5401 ccggttcaaa taaatcgtac agcttctttg cacctacttc gacagcgaca aaactactat
     5461 cggcagcaac tgttgcatta tcaaaatcct gtaccgagac taagccactg gcaattgatg
     5521 ttggtagatg tcttacatct ataacaactc caccaaaagt ttcccaattg gcaccattct
     5581 ttttatcacc ttcaataatc tgtccaattg attgcatcaa accatccgtt tcagatttag
     5641 ttatctcaaa ccgcactgat ccgttgtcac caacagttaa tgtcttaacc ccttccaccg
     5701 gttgaccaaa aacagtatat cttccatctt gtcttactag ggcttcgggt gatttttgca
     5761 tacgtgatta tacaattaat atttataaaa caagaatccc ccgcgggagc gatggactaa
     5821 ggctatgcaa gagagaaaat aaggggaaac aataaaaact aatttatatt taaaattttt
     5881 aatttttaat caacttttaa tgattaagtt cttaatttgt aaaacaaacc ccaccggatg
     5941 gcggggtatg aagttagcaa acttaaagaa tcacacgaaa tatctaacta cgaggaatag
     6001 gaccaccaaa accctttctc atatctggat ccaagagacc ttcttgttta aatttaattc
     6061 ctagttttgg ctcaagttcc gctgctagtt ccctcatgtc cgcgacctgg tttccatcaa
     6121 ctgaatctaa ttttggccga aaaccttcac cgaactgaca atctttctct acagcaaccc
     6181 tggctttgtc aattaactca gcttcttgtt tggcggatag aatatctcct tcgattttga
     6241 caacattagt taccttatca aatccttctc tgattaattc tccaacagca tgtgtgggca
     6301 tggcggtgac cataacttca cctgtgggcc tgaaggttgt attgtccatg agaagccgac
     6361 tactaataga ataattacca cgttgaccat ctgggtgttg aacaaagatc tgtgtcatat
     6421 tttttcaaat gtttaattat cttcatctta taagaaaaag aaagaaagtc aatacaatcg
     6481 tgacgaccga acaaagctta cttggttaga tgcgctactc taatcactat ctggaccaac
     6541 aggcaagtta aacagatctc ctgctagtcc atttgattca tcacctttgg caacttgaag
     6601 tcgcgccagg gtttgttcag gcgacaaagg aatacggtca ttagctaaat tggaaatgtc
     6661 aaaccaacct ttctgacctt caaaaattaa actaatgact tgattgtcgt ctgtcttgcc
     6721 gagcagatct tgatgtaaat tctcaacgac atgcctgcga atataatcgg cctgcagaac
     6781 catttgtctc ctaagctggg actcgatcaa ttcaatagac ctgcgccgat ctaaaacaag
     6841 attgttccga acacgatctt cgtcataaac accaatagct tgggttgctg ctttattcct
     6901 ggagttccag cggagttcat cagcactgac atagattccc aatgcaccag agatctttct
     6961 tctggcatca atggcttcca ttaatatctg tcctgaccac tccggaattt taccggattc
     7021 aacaactcca tcaatatacg ttaaatcatc agctcttatt tgttgcaaat atcgctcggc
     7081 ctcagcaaca ggagtttctt ttttagtcat atatatttat ataacataaa ataacatcta
     7141 atgtaataac accccccttt ggcaaacttt ggaagatatg aaagtgccac gccttcgact
     7201 tcgcttagac tcgcaatgac gtgtatgtag gcagattcat attaaggaac aaacactcgc
     7261 cagaggcggg caggctccga cttgttccta cttgggagta ggtaccgata tttagaaatc
     7321 aaaaaggtta cttgagaaat ttacaatatt taagtttctt aagatgatat tgttacttta
     7381 attgacatta atatagtaaa ataatccccc tgtgtctttt ggcgaattgg gggattattt
     7441 aaacactggt aaacttagat ttctgaatct ggattttcag taacatcgtt accaattatt
     7501 tcgaggattt tttgagtacg gtcgagaatc tgatcttcat tggcaagcat aaaatctctt
     7561 tcaatggcta aaaactgacc gatttcatcg atttgatctt cagttagatt atcgagtcgg
     7621 tcaattattt ttaatttgac agcatcggtg atccagaaac tatttttgag gagcgtcagt
     7681 aaagcagttt ttttgtccat aattagttag atctattaaa aggtaacttt ttaaatactt
     7741 cagcggcttt accggcagct tttgttttca taccaccaat tctatctttg acggctttaa
     7801 gctctggact ttctccaact atttttaaag cctcacccat aactctggcc tgttgaaggg
     7861 ttgtatcaat aactacgaga ttacctttta ccgctgcccc gaccagttca gcaccaggga
     7921 caaatacacc cgccgcagca gacagccgat ctatagtaac atttgctttt tctactaatg
     7981 cagatttttg tggagggtgt tctacaagac tatgggtgac gttattaatg gcaaggtcct
     8041 tcaatccttt acctaggcct ttgtctaacc caggaactcg attcgtaagt tctttggctg
     8101 cagcggtagt taaagtttct gctaactggg caagatgatt agcaccaata ctttttaatt
     8161 ccccaacaac agcttgtaat tgctgttttg ttgtacctct gacaatcatt tcagcggttt
     8221 cattttctgg cgtattacga ctatcaattt cagcagcaac ttgggggcca gttaaaggaa
     8281 ttatttcatc aggatgggga tattcttgtc ttggttcagg cattatttat ttaatttgta
     8341 attttatatg actatgtata ttaacaaata cccactgact ataaaatgtt tcgtgcgtct
     8401 tggcaaatca tgctttatcg gaactagttt ttaaaatcaa ttatatttta tagacaagat
     8461 agttggcacc gacgctaaaa gttgtttaag tgtcttatga tgcttagatt acttaagtaa
     8521 actcaatata aaaacaaaaa atcccccgat tgctcagggg attttgaaag ttaaaaatac
     8581 ttttattctt tatctgatcc ggtcttgatg gtcaattttt cttggatatc tcggggaaca
     8641 ggatcgtaat gggatggttc catgtagaaa gatccacgac cttgagtcaa agatctgatg
     8701 acagtggcgt aaccacggac tgcttccaaa ggaatgaaag caataatggt ggtgaaaccc
     8761 atagcatcag tagtaccgtt aatctgacct cgacgagaag acaagtctcc gataactgta
     8821 cccaagaatt cagaaggtgt ggccacctcc attttcatga taggttctag aacaagtggt
     8881 cgagctttct ggaaaccatc cttgatagca tagttaccgg caagtttgaa agcttgctca
     8941 gatgaatcga catcatggta agaaccatcg tagacagtaa ctttaatatc ggtacatgga
     9001 taaccagcaa taataccttt ag
//
LOCUS       DDWP01000066            2730 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_37218, whole genome shotgun sequence.
ACCESSION   DDWP01000066 DDWP01000000
VERSION     DDWP01000066.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_37218"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          863 a          396 c          656 g          815 t
ORIGIN      
        1 tttttttagc atgtatctat tgtagcaaaa caagaacttt cttgtctcct gaattgatgt
       61 cttctttgtg gcctgaggtg ggtaagtcgt gaatactttc catcattaaa ttatatgagt
      121 acttgaaata ggctccaaat tgagtgccaa catcatgaac tgtaaattgt tttttattgt
      181 cgtcgtaacc taaaatgact agactgtgat accagggacc gccgctttta aaatgtttgt
      241 tttcgcgata taaagttttg ccgttggcgg taacgatgac aggagtgtcg tttaagagat
      301 aacttttaat tatatctata gaaggattat caatgacttt cggggtgtaa ccatatcgtc
      361 tttgggctgc atcacccatt tgagtgagat tgatatcgtg tgactcggat tcgccaataa
      421 tggtttgata atcggagagt atttgttcaa ggctgggatt aactttctga taataataat
      481 ggacagtaag tatagaggct tcttcgcagg catcttgcca aggctgatcc cagtttttct
      541 caggagcttg agggatgaaa gtagttttga tttgatgtgt tttaggtgtg cttggttttg
      601 tagtctctgt cgggacagga ggtgattcaa tggggatgtc attaagtgta cattttccaa
      661 gaaatacgga gacaaccagg agaagtatga tttttttcat gattatattt tatcctattt
      721 tgtggtaaaa tggacgaatg ccgaaatata gtgatatttg taacattgcc gttattgccc
      781 acgtggatca tggcaagacg actttggtcg atgctttact caaacaaacc catactttcc
      841 gtgataatca gtcggagatg aatgttgaca gaataatgga tagtaatgat ttggagagag
      901 aaaaaggaat cactatttta tctaaaaata cttcagtttt ttacaaagat agaaagataa
      961 atattatcga taccccagga catgcagact ttggaggaga ggtagaaaga gttttgaata
     1021 tggcagacgg agccctatta atagtagatg cggctgaagg gccattgtca cagacaaaat
     1081 ttgtcttaaa aagagctttg gaatatggat tgaaagtaat cctggtgatt aataagattg
     1141 atagaaaaga tgctgatgta aaaaaagtgg tggcagatgc agagaacttg ttcctatctc
     1201 tcgcctctga cccagagttg ttagattttc caataattta cgctattggt cgtgatggaa
     1261 aaacttttta tagcatgccc gactctatgg atgcacccgg tgatactatt cccctgtttg
     1321 aaacaattat aaaggcgata cctagctcaa gaaaaaacat tgaattacct tttcaaatgt
     1381 tggtgtcttc ttttgagaag aatgggtatt tgggtagatt ggcggtagga agagttaatc
     1441 aaggagtgat taagaaaaat cagtttgtgt cgctggtcaa ttcggatcag aaagtaactg
     1501 gtaattttag aatcgaaaaa atgtatgtta acgaaggaat gaataaggtg gagacagatg
     1561 agatagagag tggagaaata gcttatttgg cgggaatctc agaaattgaa attggtcaaa
     1621 cattaactga tccacaatat caggtggctt taccagcaat agtggtagct gagccgactt
     1681 taaaagctag ttttggtcca aatacttctc cgtttgctag agatgagtca agatttttga
     1741 caagtcgaga attgaaagaa cgtttggcag aagaaattga aactaactta ggattgagaa
     1801 tggagaatga cccaacagat agtattagag tgctgatatc tggaagagga gaaatgcatt
     1861 tggctatatt ggtggaaaaa ttgcgtcgtg aagggtacgc catggaattg ggtaaacctg
     1921 aagttatctt aaaaacaatt gatggtaagt tgcacgaacc tttttcggaa ttaactatac
     1981 tgactcctga ggagtacgta gggacgatta cttcggagct aggtaagaga aaagcacaaa
     2041 tgctcgatat ggcgacagat gacagatcgg gagtgaagat ggtttataag attagtgaga
     2101 agaacgcatt gggtttgaga aatgcattga tgactgagac caaggggcaa gtgtcaatta
     2161 gtttcctatc attaggctat gaacttaaac aagatgaaac accacgagtc agaaatggag
     2221 cattaattgc ttctgaaggt ggtaaggctt tatcttttgg gcttgattta gctcaaaaaa
     2281 gagggataac ttttgtagga ccgactgagc ctgtctatga aggacaaata gtaggattac
     2341 gcccagtgtt gggtgaccta gaaattaatg tctgtaaagg aaaacagtta accaatatgc
     2401 gttcgtctgg aaatgatgat gctattgcat tagcaccacc aacaagatat tcgattgaag
     2461 agtccttgga ctttatcgaa gcagatgaac taattgatat cacaccaaaa actgttcgtt
     2521 tacgtaaaaa gtatttaacg aaagtagata gagttagagc cgagagaaag gggaagtaat
     2581 ttctatctgt ggttggggga caattttttc ccaatttatt tctgacatga atttttcaaa
     2641 ttgttgactt agggcttggg tccagtttag ttgagaggct agggctatag caaggagaaa
     2701 aagagccgcg gtaccgataa agctgccaaa
//
LOCUS       DDWP01000067            6233 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_28123, whole genome shotgun sequence.
ACCESSION   DDWP01000067 DDWP01000000
VERSION     DDWP01000067.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_28123"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1677 a         1309 c         1134 g         2113 t
ORIGIN      
        1 ggtaagactg ctctggctat tgacagcatt atcaatcaaa aaggcaaagg tgtctattgt
       61 atttatgttg ccataggtca aaaacaatca aaagttgccc atctagttgc tcagctagaa
      121 caagctggtg ccatggaata tactgtcatt gtttctgcct cagcctctga tccagctact
      181 gcccaaatga ttgctccttt tgttggctgt tctgttggtg agtacttccg tgatcgtggt
      241 gaagatgctc tagttatcta tgatgatctt actaagcatg cctgggctta ccgacaaatt
      301 tctctttcac ttcgtcgacc tcctggtcgt gaggcctacc ctggagatat tttctatcta
      361 cattctcgtc ttctagagag agctgctcgt ctcaatgaag agttaggtgg aggctctctt
      421 actgccttgc ctattattga gactcaaggt ggtgatgttt ctgcctatat tcccactaat
      481 gttatctcta ttactgacgg acagatatat cttgatcaag atgcttttaa tgccggtaat
      541 cgcccggcta ttgatgctgg tctatccgtt tctcgtgttg gtggcgccgc tcaagttaag
      601 gcgatgaaac aagtcgccgg tcagctccgt cttgatttag ctcagtatcg tgacatggct
      661 ggttttgctc aatttggttc agaccttgac agtgagacca agaaaaaaat atcccgaggt
      721 aaggtcatgg ttgaattact caagcaagat caatacgccg ttatgtcctt ggccgatcaa
      781 gtcctaacac tttatgctgg caatggtggt tatctagaca actatagtag cgaacagatt
      841 aaaaccaagg ttcttggttg ggttaattat cttaacgata gtcgtcaaga tttggttatc
      901 tccctccaac atgataagca cattactcct gagctagatt cttccctaaa agacgctgct
      961 aataaattct ttaaggagac tgaagatgcc tagttcacaa aatctcaaaa agagaattcg
     1021 tttggttaag aatattgcca aagttaccaa ggccatgcag gtggcagctt ctttaaaaat
     1081 gaaaaaatct caagagactg ctttagctag tcgtttcttt tcacaaaaat taagccacat
     1141 tgctagtcta gttgtcggtg aagctgttac tattatcaac cctagaatag atcttgtttt
     1201 ggttgctcca gatcgtggac tttgtggttc actcctgtcc actatcgaga aaggtctaaa
     1261 aaagtggatt gattctattg gtcaagaaaa tgttcgggta ttttgtatca atcgtaaagc
     1321 aactattgtt gctcgccacc ttaatctcga tataattggt gtttttgata ttagcgtcgc
     1381 caaaccggat cagaccaaga tgggtgcagt tattcaaatg ctcaccgacg acctcaaaca
     1441 aggtcttgtc ggtcgttgtc ttgttggcta tgctcaattt gaaaacctta tgtctcagaa
     1501 tttcaccacc actcagattt taccttttca gccagactct agtcttagtg atgtcgctga
     1561 ttttcttatc gaaccaaata aagtcgaagt ttacgataat cttgttccta ggtccatgcg
     1621 tctcaaactt tatcaaacta ttttagagac cgccgcttcc gagttctctg ctcgggctat
     1681 ggctatgaaa tcagctagtg acaatgcttc tgatatctct gtctttctta cttcagagta
     1741 caacaaacat cgtcaagcct ctatcactgc tcaaatagcc gaagttattg gtgcaggttt
     1801 atcatcagaa tcataaatat gctcaaaaaa atatcaaaaa aaagtgtggg aataatttct
     1861 caagttttgg gtccagttgt tgatgttagt tttggtgttc atcatatacc tcctatctat
     1921 cagtctcttc aagtcgaaga cactggtctg gttctagaag ttcaatcaga tttgggcaac
     1981 ggtactgttc gttgtattgg tatggatgcc accgaaggtt tggctcgtgg tatgtctgtc
     2041 attgatttag gtgaacaaat ttccgttcca gttggtgagt ctatgcttgg acgaatggtc
     2101 aatgtccttg gtaaccccat tgatggtggt gccaagatcg tctcaaaaaa gaaatataat
     2161 attcatcgtc ctgctccaag ttttgaagat caagagacta gccctgaagt tttagaaact
     2221 ggtattaaag ttatcgacct tattgcccca tttcctaaag gtggtaagat aggcgttttt
     2281 ggtggtgctg gagtcggtaa gactgttatt attcaagaac taattagaaa tgtcgccgcc
     2341 gagcatgggg gatattctgt ttttgctgga gttggagaga ggactcgcga aggtaacgag
     2401 ctctatcgtg aattaactga atcaggagtt atcaacaaaa ctgcactagt ctatggacag
     2461 atgaatgagc cagctggagt tcgtctcaga gttcctctaa ctggcgtctc tattgctgag
     2521 tattttcgtg atgaaatggg tcaagatgtt ttacttttta ttgataacat cttccgtttt
     2581 tcacaggcag gggctgaagt ttccgctctt cttggtcgag ttcctagtgc tgttggttat
     2641 cagccgactc tcgccggaga aatgggtgaa ttgcaagaaa gaatcacttc cactaaaaaa
     2701 ggttctatca cttctttaca ggcagtctat gtcccggctg atgattacac cgatcccgcc
     2761 ccagttaccg cttttactca tcttgatgct tcaatcactc tagaaagatc aattgccgaa
     2821 caggggattt atccagcagt tgatccatta acttcatctt ctcgttatct tgaccccgcc
     2881 attgtcggta aagaacatta cgaagttgcc agaggagtcc agaaagttct tcagcgatac
     2941 aaagagcttc aagacattat tgccattctt ggtgtcgatg agttaagtga tgaagataaa
     3001 ttaattgtct cccgtgctag aaaaattcaa aaattctttt cccaacccat gtttgtcgcc
     3061 gagacattca ctggccgaaa aggtgtttac gtcagcatta aagatactgt tgctggtttc
     3121 aaaaaacttt tatctggaga tcttgataac atttccgaaa gtgaatttta tatgaaagga
     3181 ggtctagacg atgttagctc agataataac tctcgaaaaa attgagtgga gtggtgaggc
     3241 cactagtgtt accttgccga ctactgatgg gcagataact attttgccag ggcaccgtaa
     3301 acttatctgc gctgttgaag ccggtgaagt caatatcaag accaagcaag acagtttaag
     3361 cttctttatt accgaaggtt gcgccgaaat caccgagaca gaagtctccg tattagccga
     3421 cctggccact aaagctgccg atctcactct agctcgtgtc gaggaagcca aaaaagccgc
     3481 ggctactgct aagctagaaa agattgacga tgttgaattt gcctctatcg aatcaaacct
     3541 tcgtcgtgaa cttgccaaag aaaaaattat tcaaaaatat aagatcaaga ctaggccaat
     3601 aatttaatct gtccaattct agttttcttc aatattttta ccttttgact gaagggtcca
     3661 tagtttctca tatatgcctt tttcttttat tagttgatca tgtttaccta cctccacaat
     3721 atttccattt gagagtacga caattttatc tgcatcccta attgtcgaga aacgatgagc
     3781 aattattatt actgttctcg attttgagac ttcctttaaa gcttcttgaa tcattttctc
     3841 cgactccgag tccaagctgg aggtcgcttc gtcaaatatt aaaattttgg gatcagaaag
     3901 taatgccctg gcaattgcca atctttgttt ttgcccaccc gacaatttga ttcctctttc
     3961 tcccacttcc gtctcccagc tcttaggtag tttttcgata aactccaata tgttggctct
     4021 ttttgcgact atctgtatct cttccattgt cgcttcaggt ttaccgtaag aaagattgaa
     4081 ttttatagtg ttgttaaaca gtattggttc ttgtggtact actcccatta actttctcaa
     4141 gtcacttttg ttaattttct ttatatttat cccacccact ttgattgttc cctcagacgg
     4201 gtcgtaaaaa cggatcaata gttttactaa tgttgttttg cccgcccctg atctccccac
     4261 aaaggcaacc tgttctcctt cttttatatt taaatttatt ttatcaagca ctctttcttt
     4321 attttttgga tagacaaacc caatattcat gaattctact ttccctttta ttttatcgat
     4381 ttttactggt ctttttgggt ctttcactat tacttcgttg tcgagaattc caagatattt
     4441 ttgtaggtca gttagacatt tagcaatatc tctaactcta aaaaacaggt taaaaaactg
     4501 gtaataaaat ccagttacaa atccagccac cattatcata tctccattac ttaagccatt
     4561 atctagctga tttactgtca tccacaacat tagtactatt cctagtcctg aagttgttcc
     4621 aattattaca tccattagtc taaatgaatc tccaaattta aaaagtttta ccgaccaaat
     4681 aataaattta tctttcaatc gatgcctttc tttcttctct gcagcaaagt actttactgt
     4741 ttcatagttg atcatcccgt cagtaattac tcccgatatc tcatcttcca cctggttaaa
     4801 ttcagttcgc gtttttaagt tcttatttac cagccaagta attaatccta agttcatcat
     4861 aaatatagtt accaatattc cagcaattcc ccacgacact gtcgacaaaa aatagacagt
     4921 taccagaaga ttaattagta cccgatatag ttcgttatgt aaattaaaaa atagtgtaaa
     4981 tacagcacta tcacctctgc gaaacgctga aattagagag ccagtatttt tatctacatg
     5041 ataggcaaag tccaactcta gcaccttttg aaacacagtc tctcggattt ctttagatag
     5101 ggggactaat attctatcgc tcagataaag cgcccatgct gacaccaagt ttcccataac
     5161 gagtgatgct ccaaacaacc ccagaaaaat tttgacttca ctataatttc ccagctccga
     5221 agctactagc attcttttta gccagtatgg actaattgtt tccagtacca cagctattgt
     5281 tgatatgaag agaaataccc caagtcttac tttatatttt cctacaaact ggtagaattt
     5341 aattattagt ttcacgcaca gcattctaac aaattaataa tgagtaatac atacacttgg
     5401 taccaaactt taatcaaacc ctcctggagt ccacctagtt ggctatttgg acctgtctgg
     5461 actgtcctct atgctattat cactgctact tttggctggg ttttttacaa agtttttacc
     5521 ggcaaattat cttggacaat tgccttacca ttctttctca atttaatttt taattttagt
     5581 ttcacttatt tccaatttgg tttaaaaaat aatctctttg ccgccataga catttttctg
     5641 gttttgatta ctatcatctg gtcttttatc gccatctggc cacattccaa agttattgtc
     5701 tatctcaata ttccatattt gctctgggtt tcttttgcca cttttttgca attcactatt
     5761 acttatttaa atagataaca tgtccatcaa gctttacttg gttactcttt ttgtttttct
     5821 cggaattgat tctatttggc tactatttgt tgctaaaaat ttttatgctg acaagcttgg
     5881 ttatctcatg gccaagaatc caaatttgat ttccgccggt attttttacc ttctttttgt
     5941 agtgggtatt attttatttg tcatcagtcc tgctcttaaa aacaattctc tatctcaagc
     6001 cattattatg ggtgggctct ttggccttat aacttatgct acttacgatc tcaccaatct
     6061 cgctactatc cgcgattggc cactggtcat tactattgtt gacatgatct ggggtactgt
     6121 actctcagcc actgttgctg gtgttagctt ttttatctac aaatggatag cctagtttcc
     6181 tctgcactac tcatctggtt ttacatgact tgttggtact ttatttctct tct
//
LOCUS       DDWP01000068            7547 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_46533, whole genome shotgun sequence.
ACCESSION   DDWP01000068 DDWP01000000
VERSION     DDWP01000068.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_46533"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2249 a         2125 c          940 g         2233 t
ORIGIN      
        1 ctcttcaccc ccatcaaagt cttctcattc caggccatag ttccatcaaa aggacccatg
       61 aacatcagat acattctcac cacatccact ccataaatat ccgctactga ctctggtacc
      121 actacatttc ctttagactt actcattctc tggttatctg gtcccaaaat caccccatgc
      181 gaggttcttt tgtaataagg ctcaggtatc ggtactaccc ccaaatcata caaaaactga
      241 tgaataaacc gagaatatag cagatgaaga gtattatgtt catctccacc aatataaaca
      301 tccactggcg accacttttt taatatatca aagtctgcta acttgtcctt attgaatgga
      361 tcacagtatc tcaaaaagta ccaatctgat ccagcccagt ttggcatcgt atctgtctct
      421 ctttttgcct cccctccaca cttaggacat ttacagttta cgaattctgg tatgcgtgac
      481 agtggactta gtccatcatc agtcggttca taagcctcca cctctggcaa aacaatcggc
      541 aagttttctt ccttttctgt tacccacccg caagcaggac aatctaccat cggaatcggt
      601 tctccccaat aatgttgtcg actgaatatc caatctctta tatgataaga agaagtcttc
      661 ttcatataat ctgtctcatc aaacgctggc ttttccccca cttcagtata agccttttca
      721 ttaaagtccc atccttcttt ttgtatcacc ggcactattt ccaaattata tttcttagca
      781 aacgcatgat ctctctcatc atgtgccggc acccccatca ccactccagt tccgtaactc
      841 attaatacat aatctgcaat ccacaatggt acttctttac cgttaactgg gttaatcacc
      901 atcaatccgc taaatacacc agtcttatct ttatttaaat cagtcctatc catatctgac
      961 tttttcctcg tttcttttac ataatcaatt acttcttttg aacctaaact ctgaactaat
     1021 tcatgttctg gagaaatcac cataaacgtc actcccggta atgtatctgg tcgggtagta
     1081 aacacttcta gttcatttgt tttaagtttt aagtttgaag ttttgagttt gaactttacc
     1141 ttcgctcctt cacttttccc aatccaatta atctgcgcca tctttacctt ctcaataaaa
     1201 tccgtatcct ttaacccatc aatcaatcta tcggcataat cggtaatctt caccacccat
     1261 tgagaaatat tccgacgact cacctcggca tgacatcttt cacattttcc atctaccacc
     1321 tcttcatttg ccagaccaat cttacaagaa ggacaccagt taatcggcat ctcctgttta
     1381 tacgccaaac ctttctcaaa taattttata aaaatccatt gtgtccattt ataataagca
     1441 gggtcagtcg tattcacctc tcgactccaa tcaaacgaga aagccaatcg tttcatctga
     1501 ctcctaaaca tatcagtatt ttctttagtc acctcttgtg gcttacgctt catcttaatg
     1561 gcatagtttt ctgtcggcaa tccgaaagca tcccaaccca tagggaaaag cacattatac
     1621 ccctgcattc tcttgaatcg agcataaacg tcccccccgg taaaagaaaa agcatgtcct
     1681 acatgaagtc ccgaaccaga cggataggga aattcattca acacatacat cttcggctta
     1741 gtcgagtcat ctttagccgc cgtagcatca gcgtaggtgg cccaaatttt ctgccattta
     1801 ctttcgattt ttttgtagtc catagtttgt attttaatat aaaaaaagcc cctcttgggg
     1861 gccagatata aaaatctccc ccaaaaacta cataagaagg agagaattaa attttatatt
     1921 cacaaatcta ttttaccaca agctaagcaa tgtgattatc tcggtcaaac atttctcttt
     1981 ccgaaacagg tgctgacaca aacgaacccc ttctccacct caactgccta ctcaccctcg
     2041 ccggcctatt taccactacc tctctagcaa ccaccctcgc cactggcaac ctagctacct
     2101 ctcttaaaag aatcaaactg ccatctctaa tctctgcttt gttaaatata atcatcctat
     2161 tattatacct cgctctagcc tatactctca ttaatgcaaa aaactctaat attcttaagc
     2221 tttggtacga ccttttttgc cttaggtttt gcggcttctt ctatttttta taacgaaaaa
     2281 atcatccaaa gctccgagat cagagcccct ggcaactata aattcattaa cccccttctc
     2341 tcttgcgata cacctttcac caatcaagat gtcaatacca gtcgtctcaa gcacaaagtc
     2401 gaacaaatag ttgaagacca aatcaacaaa aaacaaatca gtttcgcttc tgtctactat
     2461 cgagacatga ataatggacc ctggtttggc atagaagaaa aagagctctt ttccccagcc
     2521 agcctaatca aagtccctct aatgattgcc tatttcaaac aagccgaaaa tgaccccaac
     2581 ttccttcata caaaattagc caataatgta cctcacgatc ccagccaaaa cattattccc
     2641 agcactaccc tcacccccaa tcaagaatat actatcgaag agttgatcga acgaatgatc
     2701 aaatattccg acaacgatgc ctactacctc ctccataaaa atatgagaga agaaaacatt
     2761 cttcgaatct atgccgattt aggaattgat ctctctcctg ctgtcaacaa tccaggtggc
     2821 aatatcattt ctgtcaaaaa ctatgcctct ttctttcgcg tcctctttaa cgcctcatac
     2881 ctcaatcccg aaatgtcaga gaaagctcta gccatactca accagtctga gtataaagac
     2941 ggtattgtta atggcctacc taaaaacacc atagttgcac acaagtttgg tgaaagacaa
     3001 tatgaagcca ctggcgacaa acaactacac gattgcggta ttgtttattc gtctagtaac
     3061 ccttatctaa tatgcatcat gactcgaggt cgtgaattta gtaaaatgtc tagaaccgtc
     3121 cagcaaatat cctcagagat ccatcgacaa ttatctctct aatctactca tacctcagtg
     3181 catttaatgc cgaaatttta gtggctctct tagatggata ccaaccagtg agtattccca
     3241 ccacaaagct aattgccaat ataaatacta taaaaaatac cggtacataa cttatatcca
     3301 gcaccccttc ccccttcatt atcgataccg atgacaatgt caaagacaac aatctccccc
     3361 ccagataacc caacaacact ccagctgctc ctccacctac ccccataatc atcgactcag
     3421 ccaagaacag ctctctaact tcagcaatct gcatccccat tgccttcatt accccaactt
     3481 ccctagttct ctccaaaagc gacacagtca tagtattaaa cattcctaaa gatgccaccg
     3541 ccaaagcaat agtacccaaa aaacctaaca aaagtcttaa cgtcgcaaac aactgctcta
     3601 tctgggccac tgtatctaaa gtactcattg tcctaaaacc actgctttca attgcttttc
     3661 ttaccccagc cacttgcccc tgatcagccg tcaatacctt cacttgagaa taatttctca
     3721 ctcccaactt tctagcatcg tcaatctgaa aataataata attagccgaa tcatcatcca
     3781 ccaccccaat aactgtataa tcagtctcct gcgattgcag cctaccgctt ccaccaggta
     3841 tcaaaccatc aggaatgata taagatactc caaacttttg tcctaaaatt gaattattat
     3901 ccatgccaaa catttttacc aaaccagtac tcacgtaagc ctcccctgta ggtatttctg
     3961 aaaaaaacaa atcatcattc tttttctctg ctgaatcaac attttttaac tctacccatt
     4021 caactcctga ttcatctgtc gtaatcacca catcaaataa attagcctca gccgaagcct
     4081 cactagcagc gatcttagcc gattctgtcg cctcccccaa tacctcacct acttccaaat
     4141 cactatacaa atactcttcc aaagtacgat attcagccag actggcatct tctccgcctt
     4201 ctgataaatc ataatcacgt acacaaccaa tcacccattt ttggccaccc tcttcactca
     4261 tcaaaggtat agctaatccg tcctcaccta cccgccatag tggagtcttt actctcaccc
     4321 aactagacat ttctttacta gtcacactgt ccaccacaaa caaatcattc ttttgaccta
     4381 aatgataaga ttcaccccat acacgctcac ctaccaatcc accttctaag cgtattaaat
     4441 agccaaaatt atcactatca gtcgaacatt tatcgtagac atcaactcta gaacttccat
     4501 ctacattaaa tctaaatatt ccctcgtcaa tcttttgacc caaagtcgac ctaaccacct
     4561 ctacgctagc accagccacc tcaccctctt gataacgaaa ccccaccaca ttctttttga
     4621 actccgaccc agatatcaat tttgccccaa ccgcctttac atatcgatga tccaccccaa
     4681 aagccattat gtctgaaata gaattcttaa acctaacttt agacaccata cccaccaccg
     4741 gcaccacttc ttccaccccc ggcatttctt caactttttt aatgaattca tcattcatct
     4801 ttaagcttga tacctctccc aaaccaacat ctaatgtttt taactcttct aatctcgcca
     4861 ccttaccaat caccattttt tccaaaccat aacccagaga cactaaaaac actatcgccc
     4921 caacacccac cgccacccct ccaatagtca ccattgttct tacttttttc gcttttaaat
     4981 tctttattgc caactcaatc atataaaccc tcgacacacc tgcactagct cccctatcca
     5041 taactttcag cactctatta atcattctcg ataaatgacc aaacccaatt cgactaacca
     5101 ctccccccaa ccatctcacc gacatcattc cctccagtac cgcccttagc acaaataaaa
     5161 acaacaaaac tataaaaatg gcataactcc aagattcctt aaaaaatgat tttattgctc
     5221 ccattgggtt tttaatcaac atatttttta ccacttttct ttaaataatc accgtcttgc
     5281 accgtctctc cacctcgaga ccccttctta tttcttagtg tcatcgctaa ttttcttgct
     5341 cctttttcgt caatctcttc tgccaataga ccatcgataa tatgaaacaa cttagtcgca
     5401 taacgaagat attccaaatc gtgcgtcacc ataatcaccg tccttccttc atcttggttc
     5461 aaagacctta aaagttccat caattcatca cctgacactg tatccaagtt tcctgtaggt
     5521 tcatcagcca ccaatatcag cggatcaatc gtcaaagctc tagccaagct tactctttgt
     5581 tgctgcccag aagataatga attcggatga taaccatgcc aatcttcaag cttaactttc
     5641 ttcaacgcta ccatcgcctt ttcctccgcc tcttccaagc tcaaaccacg caaacgattt
     5701 ggtacacata cattttccaa tacattaagc gactttatcc atattggttg ctgaaacact
     5761 attccgatat tatttttcct aaaacgaact atctcatcgt cacccattcc atagtaatcc
     5821 ttaccatcaa catacacctt gccatcgctc ggctgttcca atcccagtaa agtatgcaac
     5881 aaagtagact ttccacaacc agatggccca aaaataactg caaaatcccc tttggctatt
     5941 ttcaaactaa taccttttat aacctctact gtctgtgttc ccaccaaaaa actctttcta
     6001 attccatctg ccaccaccaa attattttta tcagcaattg tcatcattgg gctatttttc
     6061 ctaaacttcc aaaaaatcca aaactctttg ataagctatt aaccaccaaa tcaagtgccg
     6121 acttggttga ttttggagtt actgtcaccg tatcaagtga ctcaacctga ttgcctatcc
     6181 tatccttaga gattatcttt aaatgatata cctgtgacgg ttttaaatca gtaattgtta
     6241 ctacatgatt caaggttaga ttaccatctt cctgagtcct actaggataa tcagacccag
     6301 taccttctcc ataagcaatc tggctcgtcg ccggctcatc agtattccag taaacaatca
     6361 cttgcgcctt agtcgcctca cccaccccac taactgctcc ctccactcgg atatcagtaa
     6421 tcagaggtgg tctcaaatct tctgatgtcc taaatctatt aagactcact tttgcctcat
     6481 taccagccga atctttacct ttaactatca ccacatagtc agtatcatcc aacagatcct
     6541 taattaacaa ctgatgtgtc ttttttaatg ccaaactaat ctgatctttg gccatctccg
     6601 gtctaccttc cggatagtag ctaactatcg aagatacccc agtattagtc ttccatgaag
     6661 ccataattgt agctgtcgcc atccccttaa cctgctgtat tttaactcca tcaatctttg
     6721 gcatcggtag tgtcgcaaac tcataatcat cggaagtaaa cttattacct tcctcgtcct
     6781 ctgccactat tttaagatga tactttgttc catcttccaa attatccaat ttaacagtat
     6841 aaatcgactc tgccgtcgaa gtcgttaact ccaccactga cccatatgca gttgtcgcac
     6901 catattgaac tgaagctttt gtcgcattac tcaaaccaaa tttgacatat accgaaaata
     6961 atcctaaatc agttacctta acagtagaca ccacgggggc tggattagtc tcaaaactgt
     7021 attccgttga ttcaccagta ttaccatctt catctgtcca tgtagtttta taaaaatatg
     7081 tcgtcccagg atcaaggcct gtaatcgtaa tttcatgaga actaatcaga tcagacgacc
     7141 caacttcctc accataatct ccagaacttt ttccatattt aacaaatgag ttacctttac
     7201 gattcgtcga ccaatttatc actgctgtct ttgtcttcac atcagccacc ggctcggcag
     7261 ttaacactgg cggtactcgc catttaccgt caggcaacaa agccactgtc gaacttggag
     7321 cgctacattc atttgtcgaa tcacatgatt tgacacaata ataatatttt gactgcaata
     7381 aattagtatc tataaatgat gtccctgtcg tcgaagacgc ataactaaaa tcactcatat
     7441 ctgtagtgca tgaagcccca gccaccgtac ttcgataaac tttatatgaa gctatccccg
     7501 aaccacttgc ttccggagca tcccacgata atgctagtct ccaacta
//
LOCUS       DDWP01000069            3195 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_38133, whole genome shotgun sequence.
ACCESSION   DDWP01000069 DDWP01000000
VERSION     DDWP01000069.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_38133"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          920 a          687 c          561 g         1027 t
ORIGIN      
        1 gggtgctctt ttaccaacct ttctactgta gctttccaga ttgctggacc aattcctctt
       61 ctcgtgaacc cggagtcaac agagatactg cccataaaga cagggactcc tgaggcatct
      121 ttatgcttaa gaacttctga tatctcacca ggaaatacac cgaaattagt cactctaata
      181 cacgattctg tcgcagctac ctcaccgttt tgataattaa ctagtctaaa accttcatat
      241 cctttagctg gtaattcaac aaaattgtaa tccgtcttcc tgtttttaag caaatgattc
      301 ttcctattat caatcgcctc tctaaattca gctaaagtac taccattcgg attaccaagt
      361 ggccacttaa gtttttcaaa ttctgataaa gccaattggt cgtcagctat atcagcagca
      421 acctgagaca attcagtaag caacgtaaca tttcgagccc aaaaattaat gtctttattt
      481 ctaaaggtat caaggacttc gtcggtcata ccagagcctt ccttagaata gagacaatcg
      541 cccgcacgat cataaatatc ttcaatatcg atctgtcttg ttgatatgcc attatctcca
      601 taagacagga cccttatatt cgaaccagaa cgatcaaaag gtataaaacc agcttgatca
      661 attgcttcca gttgccctgc tacataagaa agaaccgaca acttatctag ataactaatt
      721 gatgaacttt gaattaactc atcaaggtct acacctggga ggttttcaac ctcaaatata
      781 ggtccgatca actcatcatt tttctctcgt aaaatcttgg gtacaacggg ctgattatca
      841 aactggacat tctttagtag attgtaacca ataaaccgta catttcctct tccagatgct
      901 tcaactattg gcgcacgatc tttcaaatca atcattgttg aagaatttta ccaaagttct
      961 atccatgtat aacaaaagac ctctgtttaa ccacactaca caatcatatt taggctgttt
     1021 ttagaacaaa gcccattgct cccaccagta aacatattta acgttagtta tggtgatatc
     1081 aaaactgtgt tgggttacta ttgctgtacc gggtaacttt caataaagcc ttgctttaaa
     1141 agaaaaacac catttaaaga tttgacttta ttactgtcaa aatctaacaa tatagttttg
     1201 ggttgcttta ataatccaac tagcagagtt ggcttaaccc attcattact tactggaaat
     1261 ataacaaata ctgaagcgtt ttcttgtggc ctcatgtaca gttcattatc aatggggctt
     1321 aaatcttgtt taagatttct aattcttaga taatttgtta cagggataac cctcctgtcc
     1381 ccagtagtcc tcctgtcacg aatgttcaaa tgggctacaa cccaggtgat attattgaaa
     1441 ttatttctat aatcacccat atcagcaatt tctggtgtca agtatatttt atccaagcta
     1501 taggagacac tgttatcctg ccactgagca gtcactacca gttctttgat taatactgga
     1561 tttgggttaa tacccgattc tttctgtact atagtgggac taactgttgt tgttttttgc
     1621 gatgaatcgt cgttgacagg acaccctgtg ttatctaaag cttcctgata ttttgtgcca
     1681 aaataaaagc caaaaaatgg aagacccaca aataaagtca ttgctaatat ttttgaaaag
     1741 gtagttacgg tggtgaatga ttttggtagt tgcatagtta aatatcccac aggtaagaat
     1801 gctttgcaaa tagctcgata attgtctgcg atgctccccc ttcgttacac tcagggtaaa
     1861 ctcatttgct gggatagcac ccggattgga aaaatgaagt gtcaaaaaac attgctatcc
     1921 aattaatctt ttattacctt tgcacgctct tccgaccata tataatttgg ttctacatga
     1981 atacgcggat catctagatg atgcctattc ctaaacctaa gtccacgtgc cagacgactt
     2041 gcatttaatc tcagttggtc ttcacgacta gctactccaa tacctctttc atagatttca
     2101 tctaatggca ccgttacaga actcacccct gcccgcaatg aaatatctac taaaagtgct
     2161 acgcactcat atgcggaacc accatttatg acaataggtt tgccaacatt acgtttgagc
     2221 acatctccca taggtccatc ttgaggtaaa ccaaaatgcc agccttgctc ttttctcaat
     2281 ctttcaagat cactaagttc gtgatactct ccttctccca taaaaaaacc aacacctgga
     2341 gaactatcag gtgttttttt tgggcttcca gatacctgtt tctccctaag ttctattctt
     2401 gaaatacgca tacgagccag tatactagtt tacttggaca ttgtcaatat tataggtctc
     2461 aactcccctc gaagatttcc tccaaggggg ctagatgtag tttctactag cttcctcatt
     2521 ggtacgatac tcggatagaa aaatgggtga ggaaatattt agtttttggc cgggacgatc
     2581 gaaacctggt gtttgagtcg atgataggaa taggtcgatc tttggaaatc tggaccatgg
     2641 ctactttcat caaaatcggc agatatttta agggtgtagt ctggattatg tcttgattct
     2701 ggtgtccctc ttgtcttatc tttttctgta gttatgtgac ttctaataaa tttccctaat
     2761 ctctctgaca tgcttagccg atttttttta gaactaccgg taggtgactc cataatttcg
     2821 ttgtatgtct ggacagttgg acccagtctg ggagaggttg ttgaggcacg ttcactaacc
     2881 cgtctttgta atccaggtgg aagcgataaa tttggtactg cttgttgaaa tagcgctagg
     2941 gattggaggt tgtcactatg attttcatca tcaacaatga cgatatttaa accggctgcc
     3001 tgacctaaat ctgtaatggc cgctgctaga tttctattat aataccaggc atactcaagt
     3061 tcatctccac ctcgatttga tgagtattcg gcaattaccg gattatctcg gttgatagta
     3121 ccttcttgaa gttgtgctaa aagatgcact ataggaacag cctgtttatt tagggcgtcc
     3181 cagtatgtga acatt
//
LOCUS       DDWP01000070            5146 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_16823, whole genome shotgun sequence.
ACCESSION   DDWP01000070 DDWP01000000
VERSION     DDWP01000070.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_16823"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1415 a         1406 c          575 g         1750 t
ORIGIN      
        1 ggagccatct tattacatat catcccaggc ttagtctcca cacatttatc tccacaccag
       61 tcacacctca caggcacttc tttttctaca attgcacaag caccatcttg aaaaacacac
      121 gccttacctt ctggaggagc caaagcaata caagccatat ttggcttaat ttctccacag
      181 ctagttccac accatccaca atttggaata ggtgacaaag ccaatgattt taccggatac
      241 aataaacccc caaccaacaa catcaaccaa aatgtttttc tcattacaat aatctaagct
      301 tttttcaaaa acatttcaac cactagttta gccacattta tccctgtcga cttttcaaaa
      361 ccttgaaatt gacactgtcg attaacttct aaaatataag aaacatcatt ttcatctttc
      421 ataatatcca caccacaata atcaagacca cataccttgg ctactttttc agccaaattt
      481 ctatcagctt ccgacaaatc ccaaggttgg acctctccac ccaaagaaaa attacttcta
      541 aattcatctc ctaccgccga tctcttcatc gccccaattg ccttaccatc cagcactata
      601 actctcaggt cccaacgggt cggcaaacac ttttgccaca aaaacattcc caagttcttt
      661 tcctgaatct tgttcgccca tttttctaat ctctttcctg aatcaaattt cttcacactt
      721 ttaccctgat atcctttttc atgtttagca atcgccggaa aaccccaatc cctttctatc
      781 tgagatctga gatctgatat ctgagatctt gtacatatca tctttgtcgg tactattggt
      841 atttcttttt ccaaaaataa tgcatgttga ccaatttttc ccattcttgt ccaattcaaa
      901 taaccctcgt ggttggcaca aaatattccc ttttctctta aaatcctaat tgccatattt
      961 cttccttcgg tatatttccc cgacactgtc ccggctactc taaaccacaa ccccaaagta
     1021 ttttccgccg ttaattcctc tccctttaca aaaactctcg gattttcatt atttaagtca
     1081 aaactcaaat ctcgatataa agctctgtta acctctaccc ctaatttatt tgcctctgtt
     1141 accaaccgat ccacctcaaa ctggctttgt ctaagagaca ttgtgaaagt tacaattttg
     1201 gctaacatat ctatttattt aaattataat tttagccatc ccgcatgatt ttgtgcaaca
     1261 aaatccatcg ggataactca tttttttagc gatactaaat atttaactat ttctttcggt
     1321 acatctatcc cagtagcttc catgaagcct ttaaattgtg gccctttatt aacttcccat
     1381 atcaccggcc gactcttatc aaaatctcta aaagccacat ctactcccgc caccgccaac
     1441 ccgcaagcct cagttgctct caccgccaac tgttttattt cctcgggcag atcagccacc
     1501 tccacacttc ccccagccga gtaattattt ctaaattcat ctttattttg tgagcttctc
     1561 ttcatcactc ccaacacttt cttccccagc accagtactc gatagtcccc atcattagga
     1621 atatattctt gcaacatata tctcttaccc tcttctgtct cagtctttct cagatctctc
     1681 accatctctt ccagttcttc caaattatct gccttaaaca ctcttgtccc tctatcccca
     1741 ccactgcctt ttataattac tggccacttg tacgggcgga ccgatgtgtc cgcccccata
     1801 gtctcataca aataccatag actcccataa gtcgaccttg gcacatcaat ccctgccttt
     1861 tttaaggcta gcatttgata agccttacac gccatactcg gcttcccctt ctctactatc
     1921 ggatcaacta tttttggctt tagactttta gactttagac tttcgactat tagatccact
     1981 tcttcccaat gcttccccgt tgttctgaaa aacaacacat cataatcatt tatttgtaac
     2041 catccttcgt tttttacctt atcctcagtc ccgtcactca agtttcctct aatttttact
     2101 ctcaattctt ccgtatcaaa agccactcta ttataagaga ccagatccag cctaactcct
     2161 agtttcttcg ccgccttttt tagcattact aaatgctgac taatcttccc tccaaaaaac
     2221 actgctattt ttgtttggtt cttcatatta tttatttttt aataattttg gtgcttctcc
     2281 ctttttcaag gggagatgcc cgcctccggc gggcagaagg gttttcgcac taactaaaaa
     2341 tcctcccaaa tctttcctgc ccaacaaaaa ttttgtcctc aactttgatc tatctgccac
     2401 gtttactgca gtcatcacct ttttaccatt taaccaaaag gtaactccca ccaccggacg
     2461 atctctatgc ttccccaaag acgatctata gtttctataa tatagtacct tcccatcgct
     2521 tagcaaaccc agtttattgg ccaaagttct atctattgaa cttctaaatg ctccagtgtc
     2581 tattttagcc ctcacttcca ccaccctatg ttttcctttt ttaagcaccg gactcttttg
     2641 catctccggc ttaaaccctt ttggaatctt aactttaatt atttctatcg gttctactgt
     2701 cgttattttt tccttttgtt ccaccttttc aaaaaaacct tctccaaaca gatatttaca
     2761 caaagtcact gcatgatcaa tacttcttac ctctatatct cccactttct ccattctgta
     2821 tttcaaccca gctttgttgc atatctgaat tgacaaccct ggcctagcat taacttctag
     2881 taccactggc cccttatctt tatctaacac tacatccacc cccaaaaaac ccaaacctgg
     2941 aatagccttt tgacatttca ctgctgtttc taatatttcc ttccacattg gtatcttaat
     3001 tccatttacc ttaatcagct tttttttacc tatatcgtat atcttactaa ttaaatctcc
     3061 cttaccctcc accccaaatg tagttattcc tgtcgccaaa tctatcccca agcccattgc
     3121 tccttgttgc aagttagctt ttccaccact ttttgccgtt ggtatcctaa acatcgccat
     3181 caccggtact ttgttatata cgattactct aatatccgga gtacccgatt ttgttaacga
     3241 taaaaacatt ggatgaatct taattctctc ttcaactatc accccatctt gtataccgtg
     3301 taaactatat ttacccgaca atatttctct acaatgcccc ttcaaatctt ccactgtcat
     3361 cttctcccca ttcatcagtt gaaatttaat ttccccactt tcaactttta gacttttaga
     3421 ctttagactt tttctgatta ttataattcc atctcctccg taaccagcca ccggctttat
     3481 tacaaaatta ccgtctaatt cctcccaatt aaatttctcc acttcagccc aacttctaaa
     3541 acgttttatt agttttggca cccccacttc tgcctcagtc aatatctttt ttgttctata
     3601 cttactatca gcaatagctc ttccctctga actgtttttt ctcaaaaata tcttatttct
     3661 ctcatttatc gtcaaaaact gattatattg tttaagattt attccaaaca tatttaactt
     3721 tagactttta gacttttaga ctttggactt ttcctgatcg ctttcttaaa tcttccaatc
     3781 tctgacagtc ttatccctgt gtaattacca acagcaaaat taatcaatat accagcaaat
     3841 aaaaacatct ccgggtacaa caacaccatt tcttgcacct cacgtatatc catgattact
     3901 gcccctaaga tagccagaag caacgtacca aacatcagtt tctttgcttc ttttctactt
     3961 ttagccaatt cagttctcac aaattcctca gtcaacaaaa tcataaaaag catcgggaac
     4021 accgacaatc tactcacatc aaacaccctc caataagttg aagccgatat taatccaaaa
     4081 gtaccgatag taatcatcat caaatttatt gatctagctg gccaaaaatg cagtttgaat
     4141 cttcgtaaca gtacattact aaataaagac gttgccaaga tcatcccaaa cagcaccaac
     4201 ccctccacta tcccagttgc caagaatgct acagcaatca ttgtcggagt aaatatccca
     4261 taccccataa acccaaacac ataatgcaat accgacacca ctgttgccac caaaggcaac
     4321 atcaataata gtactatcgt gtttgcaccc actcctctat ctactgctat ctcacttgct
     4381 tttctcaaac tgttcagacc attccaatta ccaacccgat tatctttaac tacttgttcc
     4441 aacgggacat caatccacgg ttcatacgac ctagcccata tctctctacc cgccgccaaa
     4501 ccagctacca acaccaaagt tatcattatc aaatatttcc tcattttggg tattttttca
     4561 aacttttaac tttcagactt tatagacttt tagactgaga gagtggcgta ttataacatc
     4621 atttgcaacc agtccatagc caaaagtttt cccatattta tcgccatttt tttcaatatt
     4681 tttttcacac cttttttatc tatttatact ttagactttt gacttttaga cttttaactt
     4741 tttaaggtat aattccccta tgaaccgact tctagccgtc aatatcggac aagatctccc
     4801 tttaggtaca ggccggtctg tttcctccac ctttcccacc ttctcagtta ttgtcgacat
     4861 catcatcaga aactcattta caattatctc cgtcgttctc gttggacttt tgcttttcgg
     4921 gggcctaacc tttataatgg gcgcaggtag tcaagatcaa aaaaaggctg ctcagggcaa
     4981 ggcaattatc accgatgcca tcattggttt cgtggttact ctctttgcct atttaatagt
     5041 ccaaataatt gaaattatta ctggtctaaa aattttaaat tcaggtttat aaaatgaaac
     5101 taaatcacaa actaatttat tcttcttttt tgatttttgt gttttg
//
LOCUS       DDWP01000071            9431 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_31880, whole genome shotgun sequence.
ACCESSION   DDWP01000071 DDWP01000000
VERSION     DDWP01000071.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_31880"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2975 a         1720 c         1769 g         2967 t
ORIGIN      
        1 agggttcgac tccctctcct tccgccaaag ggtcaaagtt ctatgcgacc cacttgctag
       61 tcaagtattg tcctaacctg ttggtgtata ttctccaaat tcaacatttt tcacttaaac
      121 agcctgtaac acgcaaaaat catactttta gaaacaagat tgacaaggat ctgggataat
      181 attatatgac taaaagacaa aaagaattta ttgaatctgc tctaaaaaat ataaatatta
      241 ttcaaaacga ggtgacagcg ttgcttggta aggaaaatgt atttcaacaa gcggtcaaac
      301 cattgttaca aagtcaaaac tctttgattg cccttgaggt ttcaagatca cattatcagt
      361 caaatttaac agcaataaga agacaattag gaatacttaa tggagaaatt agcttattaa
      421 gtcttttaac aaagttaatg aactcaaaca aatggataac cgaaaaatgg tatgcggaaa
      481 tgtggttaaa ggattacaat tttgcaggtc aagatccaat aattatgtct ttcacaaaat
      541 caataccaaa tagcgaattt aaaaggaatt ttggggcaga tggttatctt gataaatcaa
      601 tagtggaaaa agatgttaaa gaattggccg acacttgtac aagaattaaa caatatacaa
      661 atgaaaacat tactcataca aaaaagatta agaagcaaat acccatatcc gagagtgaat
      721 acaaaacagt attaagcaca attgataaac taacaacaaa atatattcta ttgttaaagc
      781 aaattggaat tacattaact ccgacaattc agtaattagt ttattgtcaa ttgtttgttg
      841 tgaagtgtaa attttgtctt tattgctgac attatttgaa tcctgtcctc agctgacccc
      901 gcttttagaa catgaagtaa gtattctttg gcggtgtccc gatctatagg tattttttga
      961 ggataatcaa attggtttag atttccagca aggaattctt gatggagtac ctcgtctctt
     1021 aatgagtgga atctttcgat ttcatctttt aattgatttg ttaacacttt ttcatctaac
     1081 tttatttcat caatgtgact taccaactgg tttattaatt cttcttcggt tatatatggt
     1141 tcgtcgcaat cgtaatcgat atgtctacca caatggtagt agatgtattt tttaaatctt
     1201 ccatatttat atcgtttgta tttttcttca gctgttatcc attccccaca gctaccacat
     1261 ttacataatc ctctaaatgg gaacaacttt ttgtgccatt ctttttctgg aacataaagt
     1321 tgttgttgag ctttttcaaa tacatcttta gtaattaaag gctcgtgatt tcccttgtaa
     1381 attattcctc cgaattcaaa ttcaccatag tagtaggtgt tttttaatgt tttatacacc
     1441 ttgcttaacg ccagaggttt tccacttcgg gtggtaaacc caattttgtc gagccatatt
     1501 tttactgttc taccgctttg gcctttgttg gccacccgat caaatatttg tttaattatc
     1561 ggagctcgct ccttatcaac ttcgacatag ctgattcggt tgtagctcat gacatttaga
     1621 tacccaagtg gagcaatgtt aggtcgccat ccagcttcgc attttgctct cattcctctt
     1681 cgaacattaa ttcctcgatt atcgttttct agtttagctt gtgagcataa gatcattagt
     1741 aaaaatttct catttggatt gttcgaaaat gattgagaaa aagtccgaat ggtcgcaagt
     1801 tttccttggt ccatcagatc aactagcgtc cctaagtctc cagcattcct actcaatcta
     1861 tctggtgccc atgttagtat ggcatcaaat tctcctgctc gaatgtcgat caacaactta
     1921 ttaaatacag gtcttagccc cgaagatttg gctgagtgac tttcttgtcg aatttctttt
     1981 atgaataaat tctctcgttc agcaagtgtt ctcatttcat taatttgaga ctcgattgac
     2041 attgcttgtc gttcgtctga ttccgatgat tttctagcat aaaggcaata tcttgtttgc
     2101 tttatttcct gtatctttcc atcttctttg ttcttcatga cactactgta ccgctatgtt
     2161 tgtggaagtc taggagatga atactatgcc atgtaggact tgattattga catcaaacca
     2221 taatgttact acactctgta gtattataaa tttataaata attataatga ttagaaaaaa
     2281 acaaaaaaat cctgaatctg actctattaa cacaattgaa aactccaaaa attatattac
     2341 tttaagtcat cccagtttgt atttttttag tactcttttg ttggtaatga cgtttgttag
     2401 tggttatttt tttggaaaag ttaatggtac tgaaaagacg tctccaacag caattgcccc
     2461 atcccagcaa gcacagcaac aagctcaaca gccagctaaa ccacagatat caatagatac
     2521 tatcaaagac ctatttgcca aaaatgtggt taaattcgga aaaggtgata aaaaactatt
     2581 gatggtaatg gtgtctgatc caagttgtcc ttattgtcac gtagctggag gagcgaataa
     2641 agaaattggt gccaaaatgg gacctcaatt tgcagcaggt acatatgtcg ctccggtgac
     2701 agaaatgaaa aagcttatag atcaaggaaa ggcagatttt gtctggcttt acaccaatgg
     2761 tcacgggaat ggagagttgg caacaaaagc catttattgt gctaacgatc agggaaagtt
     2821 ttggcaagct catgatctat taatgtctgc taagggttac actttaatta atgacgaagt
     2881 caaaaatgat attgcaaaat cacaaattat ggccgatttc ttagcctccg ctgttaacaa
     2941 gaatgaacta aagagttgtc ttgatagtgg aaaatataac aatcgtttgg ctgaagattc
     3001 agccttggca acatcgttgg gtgtaaacgg aactccggga ttttatctaa acacaactaa
     3061 ttttgccgga gcatacagct ggactgacat gaaatcagta gctgacgccg ctttataaaa
     3121 ttatgtcttt attaaaaata cggaaagatt ttactttctt agatgacaat aaatggatct
     3181 atcttgacaa tgcctgccaa agtcttcggc cgaggcaggt tgttgacgca atcaaagagt
     3241 attacgaaaa ctatcctgtc tgtgccggta gaagtaacta ccgtctcgca gtcgaattac
     3301 aacaaaagtt aactgacgtt cgtctgttgg tttccaaatt tatcggagcc aaaaaaccaa
     3361 ctgaggttat ttttacccgt aacaccaccg aaggaattaa tttgattgct aattcatatc
     3421 catttgttga agatgatgtg gtattaatca gcgacaaaga acataattcc aatcttgtcc
     3481 cttggttgct ccttgctaaa actaaaaaaa ttaaagtcga aattttctca accaatatag
     3541 acagtacttg gaactgggaa tcatttaatc aaaaactaag cagtaaagta aaaatggtgt
     3601 ctttaggaat tacttccaac ctagacggag ttactatacc atttgaagag attatcaaaa
     3661 aatcacatca gtttggagcc aaagttcttt tagatggagc tcagtgggct ggtcatcgtc
     3721 agacagaagt tagtaaatgg ggtacagatt tttttgtatt ctcggggcac aaaacattgg
     3781 gcccaacagg aaccggtgtc ctatatggca aggaagaaga attggcaaaa ttgcaaccat
     3841 atcaggtggg tggaagtacg gtagccgata ctagttatac tgattttgag ttacttggta
     3901 ttccagaaag atttgaagcc gggcttcagg actatagtgg cattctagga ttaggtgagg
     3961 cactacgtta tcttggatca attgcttgga gtgaaattga agctcatgaa aaaaaattga
     4021 cagaaattat tattgaagga ttagagaaaa ttccagatat taatataatt ggaccaaaag
     4081 aaagtgataa aagaaatgga attatctcgt tttaccatcc aaaagcccag tcgcatcaga
     4141 tttcaataat attggatcaa acggcaaata taatggtgcg ttccggcaga cactgtgttc
     4201 attcgtggtt taatgatcga ggtgttaaag attgtgttcg ggcatccact gctttttata
     4261 acacaattga ggaggccgaa agatttgtaa acgaattgaa aaaagttatt ggagtactaa
     4321 cttaatatgt tttgcattgt tgctttttta gtattatcga taattggtat tttttcggcc
     4381 tcacatcggg cattggccaa agaagccttt gactgtgtct ttcgacggat aactttccga
     4441 ccttgcaata ctggtttttc ggaaaaaatg aaggcacaat taattggaaa aatgatgacc
     4501 aaatctatcc gagtagcccg gtttttaaac agaaatttcg aattgctttc atggattttg
     4561 tttattttga ctgtcgccag cctattttgg actggtaagg gtctctataa cttttatatg
     4621 tatggtagtt gtaatggtct gaataagtca ggattttgtg cgctggatcc aaccggagaa
     4681 aataataaaa tttcccaagc tggggggagc tgttctattg gcagtggaga cgaaagcaaa
     4741 gtcactttga aagacgttaa cttgtctact tttccaatta aagatattgg cagcaaggat
     4801 aacgtagtgt ttattggctg ttttaattgc gactacacca gaaaagcata tccaatattc
     4861 aaggagtttt tgagtaaaaa taaggtcaac tacacctttg cccattatcc agttaaaggt
     4921 gaaacttctt atcttcttcc catcggttat tgtgcctatc agcaagataa agaaaagtat
     4981 tggaaatttg tcgataaatt atttgaaagt agcaaggaag ataacgccaa gccggaatat
     5041 gtcggtaaag tattaactga tttgggttat tcatcagaat ccatcactaa gtgtactcaa
     5101 tcaccagaga ccaaaacagc tgttgccaga caaagaattg agttagagaa aacgaatata
     5161 tatggcacac cattagtatt tattaataat cgaggcctag tcggcccaaa accttttcga
     5221 gtttacgatc gtatacttaa tggtttaaaa ttttggaaat aaaatgaaac aagaactaca
     5281 atattcttcg ttcgaaaaaa aactttttac tggagtcatt atcttttcag tgttaatact
     5341 cacctttggc atgtttttta tcaataaaac atcatcggcg tcaacaataa ataatgaaca
     5401 agctaaagaa attttaaccg tcaagaatga tgactggata aaggggtcaa ataacgctcc
     5461 aattacaatt gtggaatatc tcgattttga atgtgaagcc tgtcgagtct actatccaat
     5521 caccaaacag ttaaaagaag agtataaaga taaaattagt tttgtagtca gatattttcc
     5581 cctccctgga cacaaaaatt ccatgacctc agccatggca gcagaaactg ctgggaaaca
     5641 gggtaagttt tgggagatgc acgacatact ttatgataac caaaaaatat ggggtgaaaa
     5701 acaggtggcc gatccggtat tatttgtgga gtatgccaag caaattgggc tagacatgga
     5761 aaaatataaa aatgatatcg gtactcaaga aatcaaagac cgaataaatc gagataagaa
     5821 tgaagctaca aatttgggta tacaagggac acccaccttt tttataaatg gcgagaagat
     5881 tcctaatcca aaaggctatg aggattttaa gagtttaatt gatggcctaa tgccaaataa
     5941 ataaaatgcc ggataattgc tgtgcccacg aactaacttt tacaccagag caacatcaaa
     6001 gtataaaaaa agttctctgg gttgtttttg ccatcaattt aagtatgttt tttattgaaa
     6061 tcggagcggg gttgattgcc cggtcgagca ccttgactgc agattctctg gacatgttgg
     6121 ctgatgcatt tgtttatggt ttaagtctct atgtattgaa acaaagtcaa catatgaaag
     6181 cccgagcctc tttgttaaaa ggtgtcctaa tgtcgtcatt gggtctttac gtcattgttg
     6241 attctttttt taaaatttat catccagtta ttcccgtagg agcaaccata tccatcatcg
     6301 gagtattggc attaatagct aatctaatca gttttgcttt actatcgaga cataaaaaca
     6361 gagatataaa catgaaatcg gcttggattt gttcccgaaa tgacgtcact accaatatag
     6421 ttgtaattat cgccggattt tttgttatat atttcaattc tatgtggcca gatattatcg
     6481 tcggcttggg tatgtccttt attgtattgc actcatcctt acaaattgtc cgagaatctt
     6541 ggcaacaaag ccacgtatcc taaacattta ttaggtcttt ttcagaacgt tttaaaaaaa
     6601 ccgcattagt agccacgata attgaagaaa cagacattaa caatgctgaa acttccggac
     6661 gcaaagtaat tcccatactg gggtataaaa caccagccgc taccggaatt gctaagacgt
     6721 tgtaaacact agcccaaacc aaattttctt tcattttacg aactgtggct ttactcaatc
     6781 gaatggctcg taaaatatcg gccggatcag atttcattag gactacgtta gcggtttcaa
     6841 tggcaacatc agtccctgcc ccaatggcaa tgccaatatc ggcttgagcg agagctggcg
     6901 catcattaac cccatcgcca accatggcca caaatttacc ctcgtcttga agtttcttaa
     6961 cataattggc tttatcttct ggtaatacct cagcaaagat acgagtaatt cctaatgttt
     7021 tgccaatatt ttcggctgtt ttttgattgt ccccagtaat catcgccacc tcaattccca
     7081 tttcctgcat ttttttaacg gctattgacg agctttcttt gactggatca gccaccccaa
     7141 caattccggc aatcttatta tccacagcca ataacataat tgtattgcct tcgtctagta
     7201 atttttgaac cgatgattct atcggtttaa tatcaacgcc tctgtcttta ataagtttta
     7261 aattgcctac aattattttc ttaccatcaa caactgcctc aactccatga ccagaaattg
     7321 ccttaaaacc tttgatgtca tctgaaatta ttaatttgcg tttttcggtt tcagtcaaaa
     7381 tagctttact tagaggatga ccagacattt tttcgactgc cgcatcatag gttaaaactt
     7441 gatcttcact aaaaccagca gaggcgatga cttgagttac aactggtttt ccttcagtca
     7501 aagtcccggt tttatccatg accacagcat taattttgga tacttgttct agagttgccg
     7561 catctttaat caaaatatta tgtttggcac ccaatccggt gccaacagcc acggcagttg
     7621 gtgtggccaa acctagggca tcggggcagg caatgacgac tgcagaaata gcaaaagtta
     7681 gggcgaacaa taatggttgc cccaaaacaa aataccaaat gacaaaagta attaatcctg
     7741 aactaacagc caaaacaact aaatattgtg ccgctttgtc ggcaattctt tgtccgggtg
     7801 ctttggagtt ttgagcagtt tccactagtt tcacaatttg agccagagca gtgtccgatc
     7861 caactttagt agccttaaat tttaaagaac ccatttgatt aattgaacca ccaataacta
     7921 gatcaccaac ctttttctcg accggaattg attcacctgt taccaacgat tcatcaattg
     7981 atgtctcacc ctcaatcact tctccatcaa ccgggatttt gtcacccggt ttgataatta
     8041 gaatgtctcc cattaaaact tctgccgttg gaatagttaa ctcctgaccg ttacgaaaaa
     8101 ctctagcctg aggtggtacg agatcaaata aagcccgtag tgcatctgat gtccctcgtc
     8161 tcgatttcat ttccatccaa tgaccaaata gaacaaaggt aaccagcatg gcggctgcct
     8221 caaaaaatgt ttcaccaccg ataaataagg tgatgacgac actaaaaatg taggcggcca
     8281 atactccggt ggcaattagt accatcatat ttaaagtttt tttcttaagt gcttcgtatg
     8341 caccaacagg gaaaattgag ccgaatttaa aaactatcgg agtaaccaac agcagtaata
     8401 gccaattttt aggaattgag gcaggcaaag gcagatatgt taaaagcaaa gtgggtattg
     8461 taagtaacag tgaccaaaga aaacgattcc gcatatcttt ttccataaac tcggccattt
     8521 tcgggtccgt catggctgat tcatgatcag catgagacat tttagacatg tcgtgatgct
     8581 ccattttaga atggtccata ttttccatac caaccatatc ctgcatttga tgttgtgtgt
     8641 gatccatgat taataatacc ttattgttga catctaaacc tcaagatact acactgtgta
     8701 gtatatgaat aaatatttaa actataaaac atgtattttg gcggtagtgg cagtactggt
     8761 cgctgtttat atatttaaag tacagtggaa tgtaatcttt actttaggat tactgctact
     8821 ttgtccccta atgcacgtgt ttatgatgaa gggaatggga catgatggta aaagttgcca
     8881 cgaagacaaa aaacctaata caattaaata attaattgtt tattaaagaa atgaaacata
     8941 gaatcttggc gacagcggca gcgatggtga cctttgttta ttttttgtta tgctcgataa
     9001 ttacaaattt gtttcctgaa ttctctttta gaatgtatgc cttggcgttt cacggaatta
     9061 aattgactaa tctacaggca gtggcattga caacaccgga aatgttgatg ggggcgacgt
     9121 tttatgctct tatggtgtgg ctactagtat gttcagtggg ttggatttac gaaaaactgt
     9181 ctaaatgaaa cataggtttt ggcaaattga tttcgtccga ggagtagcaa taacaggaat
     9241 ggttttctat cattttttag ttgatttgga gatgatttat agcattccga ttggggtcta
     9301 taaaatacca gtggtattgc tggctcgagt ggtggcaaca atttttatat tattagtggg
     9361 agtatcgggg gcgacaaaat tcgaaataat taaagatgcc ggaattaaaa aggtatcttt
     9421 gagttttatc a
//
LOCUS       DDWP01000072            2630 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_24699, whole genome shotgun sequence.
ACCESSION   DDWP01000072 DDWP01000000
VERSION     DDWP01000072.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_24699"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          947 a          511 c          464 g          708 t
ORIGIN      
        1 cagctacttt tcaacaccct ttacataaaa aaaaagcctt ctttctgatc ttcatccgcc
       61 aagataaagt gatcagttgg aaggctcaac cccaaaaagt caaaggagtc aaaatgaatg
      121 tagacaacaa accagcgaaa gtcaagaaca ccctaccctt tggggggcaa gcccgaataa
      181 catgcccccc tcaactagcc aatggacttg atactttaaa gatatctttc tgggtcgaat
      241 gggaagatca ttcttttctt gaagaactgg aaaccttaaa aagacaagtt caacaacttc
      301 aacatatcac gtcgctacct tttggcattt atacttttcg cgtacagttg gacaggtaat
      361 atgtggcccg tatcaagatc ggaagaaatt tatgaatact ctatgcaaaa aggctggagt
      421 tagatatttt cgttttcatc ccttacgaca cgcaggagcc agcttaatgg atagtatcaa
      481 tataccactt gctgaaatcc aaggaatact tggccatgaa aacagaaaaa cgacagaagg
      541 atatattcac actacccgcg gcagatctgt tgaagtcatg agggcctttg aaaaggcact
      601 aaagtctaat atttaaaaat atctcagata ttatattaac tctctatttt gcggaaacat
      661 tagaattggg ctactagtaa taaatttcgg cattctgttt ctatcccgtt ttgaattttt
      721 aattcccaaa ctaagaaaat ctgattaaaa acaagtaaga aaatccttat gggaaaacaa
      781 taattcacac cctctgttgg gaatagtgaa ccagcaaaag atatttgttt ttattatgtt
      841 aagttagaac aaacccaaaa acaagagttc agacagaaca attggtttga ctttttatcc
      901 gttacggata atataccttg actgttgatg acagatgcct aataatttat ttgaaagatt
      961 caagattgcg tgtagaaaaa atccagcaga tgtgcttgtc gagccaaaag ccgttaagga
     1021 tgccagagag atttaccaat tatatactga gaaggaattg ctaaagttta taggtaacga
     1081 tggactcaag aacgtacgac atctgaacat aacagaatgg cgcaataatc cagacaaaaa
     1141 aaacaacccg cttgatgtgt acgactatca ctgtaagaca aacggcatac cttattatat
     1201 cgcaataata tttattccaa ccttctttgg aaagacgatc aacaagtgga aactaaaatc
     1261 gtttcacttg cctgatgatg ctaatttacc aatgtttgac caacttgcaa aactttccca
     1321 ttcacctttg gaggacaatg atgagtgaaa gtaaaatatg ccctctgtgc ggttccgacc
     1381 aagtggagca aacagaaaag ctcgaaacga tctcagatag ttttggtgaa agtactgagg
     1441 ttaaactggc ttacgatatt tgcaaacgat gtggtatgga tggtgatttt accggctgca
     1501 acaattctat aatacaagaa acaataaatg ccctaaagtc acaaacagtt atatctattc
     1561 tacagtattt ctccgaccac aatgtcaatt ttgcaggaat ggaacgcgcg cttgaactgc
     1621 cccaacgaac tttgtcaaaa tggaaaaacg gatcaattac accaacggca gcaggtgtga
     1681 cattagtgaa atttttaaga ctattccctt ggcttacaat agtcgctgaa aacagatttc
     1741 atcttaatac cccactgcta actcatatca ttgagtcggt caaaaagata tctccaactt
     1801 atggtattac ggatgatatt gggacgtcag ctccaatcgc tgtggacgcg gaatacacat
     1861 gtattaccct tggaaagaca tatgattcat taagacaaga taatgctcaa caagagcgta
     1921 tagatttggc ttggctataa taactatact atttatgagg taattaaata tgacagacga
     1981 taacacgcca caaacaaacg aaattacaat acttccaaat aacttattgc caaatatttg
     2041 ggtggatatc ttgcgtttag ccactaggca tgatggagtc tgctcattaa gtttcagtgc
     2101 tagtttacca agcgtgaacg cggaacaggt gcgcataatg acaagtcgaa acaacttgca
     2161 agcatttgtg aatttaatat gtgaaaaact tgattactat ccagccaaag agcacacaga
     2221 agagcaaacc cactgactca taaaaagtcc gccaatattc gctactatgg ccactattat
     2281 gagttcgtaa aattcgaaga ggcaaaattc taccaaaaaa cctcattaaa aggcttttga
     2341 aagattgcta catattaaaa aagagcaggc ttcatataaa aatcacaaac tttttcacac
     2401 acaacaaact tgaacaagaa agtaaaatgc caacaggaca aaacaaaaaa gggattaaca
     2461 agctaatttc gcttattaat tctactttgt cacattaaaa tatcatttaa catcttgaaa
     2521 tataaagcat tatgtgctat tatcttttgt agaaacagta aggaaagtaa gtcttccaat
     2581 cattgcaaaa gaggtacaaa tgagccacat gccaacccca aaaaatccag
//
LOCUS       DDWP01000073           10853 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_25462, whole genome shotgun sequence.
ACCESSION   DDWP01000073 DDWP01000000
VERSION     DDWP01000073.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_25462"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3408 a         2776 c         1387 g         3282 t
ORIGIN      
        1 cgatcagttc gataaaaacg gtaaagcggc acagctccac cactcaaact aggattagct
       61 acatatgaca aaccttcaaa gttccaggtg ccagatgcat cattatttaa aacacttact
      121 atctcatctg tactggcact ataaaagtga atatctctgt cagttcgata aaaacgatag
      181 acaccaaaaa cagctgagtt taaatctaaa taactctgca ccaagatttt atttaggttt
      241 aatctcttcc ccgaacttgt tttccctgca aggctagata cagagtcact atagctcaaa
      301 atcgcactct taatttggtt ataggtaaat ccggcatatc tactcataag tgtggcaacg
      361 gctcctgaca catacggtgc tgccatagag gtcccttgca taaaatcata ctgatcatca
      421 gacccctcac taaatttatc aatagtcaaa gcatcgattc tacatccatc atagttattg
      481 tcagaagaat ctgttaccca tctaaatcta aacttgaaac ttgaagtaga gaacgaatcg
      541 gggatactaa acgtcaaata ctgggcacta ccacccaaat atgtttcatc ccaccttgaa
      601 atttgagtaa aactgacccc accgttggca ctgtattcca aagccatgta atctttccag
      661 ctagaactgg tctgcggagt gtcacacacc gtccaaaaac taatactcgc tcgagataaa
      721 gacaaattat aagtgggaga gtcaattaca tagttagcgt tattttcata aggataagac
      781 aagtcaccat acaacacttt tccccaagac ccccctaaat tataagtacc ccaattacca
      841 gttttactcc acccgtttgg aatatttggc atcacaacac cctcaaagtc ttcatacaaa
      901 aaaacagctt tgctcggaac agtactatag atgttcgtcc ccggagctcc gacatcaaca
      961 gatacctccc cgtaatttga aaagctagcc aaattatcat tctgatcagt ggcggcaact
     1021 gaaattatat ttgccaaaga atagtttgct ggatagaaag gaatgcctcc gtcattattt
     1081 cttgaatcat ttccagcagc ggcaacaaat acaccccccg cattcataaa cctatttatc
     1141 gcatcatatt cggtttgaga ataataatta tcaccataac tagcattaat aacctttgcc
     1201 ccattttgaa tagcaaaatc aatcgcttta acaatctctg aaactgtcaa gtcagactta
     1261 agagccatga tctttgcctg ggaggcaaca ccaacaaccc ccctcgagtt attcttctct
     1321 gccgcaatta cccccgcaac atgagtgcca tgaaaagaag aatctgggag gggtgtcttg
     1381 tcatcaccct caaaatcata tccgtagttg cacccaccaa taaatacacc atcatcgtcc
     1441 ttgcaattag agccatccca catattatta attaaatctg gatgattata ggctacacca
     1501 ctatcaatca ctgccacgac aacaccagaa ccttcagata aattccaagc tagaggggca
     1561 tctatatctt tatccagaac accgacgatt ccactaacac cttgccctac gttatctaaa
     1621 ccccaaagat aacttttata cgtatcatta gtatttataa gtgcaggttt acgggtgtag
     1681 tttggctcag catactcaac gtcactattt ttccttagct cacttatcgt ttcctcaact
     1741 gatttctttg ttcttaaaaa ctcaatattc aatcttttta gttgaccccc atcttctaga
     1801 gacaacctgt cctttatttc agcagatttt aataatccgc ttcgcgtttt taaatcaatg
     1861 ttattttcct tatacttcac aatcacttct cctggtacat acttatttag taaaccatct
     1921 cctattacca aaagatcacc acgcttatca agactcggca aatttctatc ttgggcgtga
     1981 acttgagaat tcaccaggac caatataaag aagaagactg aaaaaacaaa cctaaaaata
     2041 tatttcacaa cacatatata tcacaaaaat atcctaatta acagcttttt cttatgatct
     2101 tacaaaaata gaatctgtcc ccgacattac ctgcccaccc caacatattc aaaccccaac
     2161 tctttcattt tgttcggatc atagatattt cttccatatt aagtgctacc ggatatcttt
     2221 gtataacccg ttaatttgct ctaccaccca ttctcaattt gtcctgattc tcaagataat
     2281 atttgtacat tttctgcaaa ccactttcta gattaatttt aggttgccaa cccaacgcca
     2341 aaatcttaga aacatctaac aactttcgcg gcataccatc tggcttggtc gtgtcaaaaa
     2401 tcaactctcc ctcaaaatta accacgtctt taatcatata tgccaagtcc ttaatagtga
     2461 tttctacccc cgtacctaca ttaagaaatt gtttaccttc atatctatcc atcaaaacaa
     2521 atatcgcctc cgccaaatca tcaacatata aaaactcacg taacgcctta ccagatcccc
     2581 aaattgacac acttctatcc tcgttcatct ttgcttcatg catcctcctc atcagggcag
     2641 atatgacgtg tgagccattg atatcaaagt tatcattctc accataaatg ttggtcggca
     2701 tacaagaaat aaacatccga tcatattgtt cataaatttt ttcacacaat ttcattcctg
     2761 aaatcttggc aatcgcataa ccctcattgg ttggttctaa cttacccgtc aacaaagact
     2821 cctcttgcat cggctgggga cactctcgag gatagataca actgctcccc aaaaacaata
     2881 acttcttaac atcatgtttt aatgctgacc agataacatt attttggatt tgtatatttt
     2941 cccacaaaaa atctgcttga tgttctgaat ttgccttaat accgcccacc ttcgccgccg
     3001 ccaaaattac atattctggc ttctcttttt caaagaaatc ctctaccgca gcttgattca
     3061 acaaatcgag ctcttccctc gtcttatata acaaattact gaacccctct ttctcatatt
     3121 ttcgcaacac tgcactccct accatacccc tatgtcccgc tatatatatt ttggaatctt
     3181 tattcatttc ttattactac tttctttttt ataatcacta tcaaccatca ttctaactaa
     3241 tttcttaaat ttggtccgag cataccagcc caacttttct tttgccttgg aagcatctcc
     3301 cactaataga tcaacctctg ctggccgaaa ataaattggg tctatctcaa ttattatatc
     3361 tccagtcttt ttattaattc ccttttctct tattccattt cccttccact ccaaatctat
     3421 atcaagcact ttacacacct cttccacaaa ttctcgaact gaatgtgttt ctccagtcgc
     3481 aataacataa tcatcgggct tagactgttg caacatcagc cacatacatt caacataatc
     3541 tttagcatat ccccaatcac gttttgcctc taagttaccc aaatatagtt tttgttcttt
     3601 acccagttta attcttacca acgctttagt aatttttttc gttacgaaag tctcgcctcg
     3661 ccgaggagat tcgtgattaa acaaaattcc attacaagca aacaatccat aactttctcg
     3721 ataattcttg gttatccaat aagcataaac tttagcacag ccataaggac ttcgtgggta
     3781 aaaaggagta ttttcacttt gtggtacttc cacagcttta ccaaacattt ccgaagatga
     3841 agcctgataa aattttgtct taatacctga ctcacgaatc gcatctaata tcctcaaagt
     3901 ccctacccca gttacatcac tcgtatattc tgggatatca aaactaaccc gcacatggct
     3961 ctgtgctccc aggtgatata tttcactcgg tttaattttt tcaattaaac gagaaatact
     4021 acttccatct gacaagtcac caaaatgtaa aaacaatctt gccgtcttct catgaaaatc
     4081 tttaaaaata tgatcaacac gactggtatt aaaagagctc gatcttctaa ctaagccatg
     4141 cacttcataa ccttttttca acaaaaactc agccaaataa gatccatcct gccctgtaat
     4201 tccagttata agcgctttct ttataatctc ttgcaataat tattaattaa tctcactttg
     4261 atacaaaaac tattgacttc tttactctct ccccacactc acataatcaa aaccgagatc
     4321 ccgcatctta gctggatcat aaatatttct accatccacc aaccttttat tcttcatttg
     4381 catattctcc cagtcaatat tcttaaactc atcagaatta ttggtcacca ccaccacctc
     4441 agcttccgcc actgcttcct ctatactttc taccaacaca ttctcaccca gtactttaat
     4501 agcttgctct tgagctacct tatcataggc cactactgtt gccccttctt gctgcaaccc
     4561 tttaattaga gctagagcag gggactcacg cacatcatct gtccctgctt taaaagcaat
     4621 tcctaacact gccacttttt taccctgaat atctccagcc agcatttttt tgatcttttc
     4681 tatggctatc aacttttgtg attcatttat tttctctacc gattctaaaa tactcatatc
     4741 cactcctacc tcatttccca tttgaattag gcctttaaca tcttttggaa agcaactacc
     4801 accataacct attccagcgg ccaaaaaagc cttcattcca attctttgat ccaacctcat
     4861 tcccttagat acatcaacca catttgcccc taccttttca caaatattag caatcgagtt
     4921 gataaatgaa atctccgtcg ctagcagagc atttgaagca tatttaatca tctccgctgt
     4981 cttaatactc gttaccaata tttctgtctt tagcccttcg tacatttctc tcattacttt
     5041 ctctgcccta gtgctctctg ccccaatcac tatcctgtca ggattagaaa aatcatcaat
     5101 ggctgtccct tcttttaaaa actccgggtt tgacaccaca tcaaactcgc ctgcataatt
     5161 ttctttaata attttctcca ccacctcacc agttcccact ggtactgttg atttatcgac
     5221 aataacttta tatccattca tcgacttggc aatcatttct gctgccgact taacataatc
     5281 caaattgacc gagccatcgt cacgcgaagg agtccctact gcgataaaaa caatttctgt
     5341 ctcttttact gctttatcaa aatcagtggt aaacaccaat ctacctgctt ctacattttt
     5401 cctaactaat tcctctatcc ctggctcata aattggcatc accccattta aaagcatttc
     5461 gatcttggct ttatcgatat ctaccccaac cacctcatgg cccaaatcag ccaaacaggt
     5521 gcctgtcact aaacctacat aaccagttcc tattactgaa atctttttca tatgctagat
     5581 attaacactt tcctcccatt tccaaccagt ctctactatc tctcgcaaat cttgatgtat
     5641 tggctgccaa ccaagcactt ctctagcttt tttactatct gccattagaa tcgctgggtc
     5701 acccggacga cgctccgcct caactacttt aaaatctacc cctgttacct ccttggctat
     5761 gtttatcact tctttaaccg agaaaccatt tccattgcct aaattaaact tgtccgaagt
     5821 acctccattg ttaagatact cgactgcctt tacatgagca tcagccaaat ctgacacatg
     5881 aatataatcc ctaatacaag taccatccgt cgtctcataa tctgtcccga acacttttaa
     5941 ttctggcttc ctccccgaca ctacatcaaa aattactggg attaaattag cacaaccacc
     6001 cggccattca cctacttcta cttctggatc ggccccagcg gcgttaaaat atctcaaagc
     6061 cacatatttt aatccgtaag ctaccgaata atcttctaat atctcctcca ccattaactt
     6121 cgtcctgccg tatggattaa taggagactt gggatgtttt tcatccatcg gattatattg
     6181 aggaactcca tacaccgctg ccgaagaaga aaaaatgaat ttacccacac catgcttttt
     6241 cattactttt aataaagtca aagtattggc cacattatta aaataatatt tctccggatt
     6301 gaccactgat tcaccggctg ccttaaacgc accaaaatgc ataactgtct caatcttatt
     6361 ttcactaaat attttttcaa gcactgcctc atcacccaag ctacctacta ctaattttcc
     6421 ccatttaacc aaagcttctt ttcctgatga caaatcatcc aatatcaaag tctgatagcc
     6481 ttttttatta agatctttat ttatatgtga accaatatac cctgctcctc caacaattaa
     6541 aatcatattt ttttaatgaa ttaataataa aagtccaccg actaacgcat taagtattcc
     6601 aattatccct ccaaatgtcg atactaatag tatcttctca tcagcaaaac ctaattgaga
     6661 aaaaaagtat aagaacaaat tctctcttgc cccaaacccg gctactgata ttggcaacac
     6721 caatattaaa gagattatgg ggacaaacaa atatacttgg acaatactaa tacccactcc
     6781 aaatattaag ctataaaacc atgtcggcaa catccacact ggctcggcca atatcgatat
     6841 cgcaaaacaa gccaaaattc ttttcttatt ctcattcttt aaaagataaa caatctccaa
     6901 taattttgct accaaccgat atttatcact ccattttctg aaaaatttat caaaatcaat
     6961 agtaaacacc aacacataaa agctagttac ccctactgcc aacccagaaa acaaccacca
     7021 caacatatcg gggaattgat aattgagaac tttaccaacc accaaggcca caaaacccac
     7081 caaagtaaaa gccgagaaac caataactct gtctattagc gccgaggcag ctaattttgt
     7141 cttcgataat tctgggtaat tcttagttaa cgacaaccat ttgatcatat ctccagctac
     7201 cattgtcgga aaaaatagac tatagaatcc acctaaataa gttgcccgag taaaattcca
     7261 aaaatctttc cagtcaggct tctctattaa tagtactgcc catctaattc caccaataaa
     7321 cattgagatt gcactataaa atatcatcgc cgctacgaac catggaggca cactcgacag
     7381 ttctcccaac aaacttccca catcaactcg gctaaaagcc caatatatta gccctatgga
     7441 caaaactaag cggagaattt ttgacgacag tatttttttt aacataaact gtaaactata
     7501 ctataacaaa atggcccgct ttttattagt cacacatatc taccctccag ctatcgacgg
     7561 aggttccagg gttatcgcca aaatggggga gtatttccgc tcacaaggcc atgagattat
     7621 ggtcttaact agcgacgcca cctccaccga tgattttacc catgcttatc aaagagtgaa
     7681 caaccctggc ttaccggtat acaccacttt ccatcgccct cttaaattaa tttccaaagt
     7741 tcttagtaaa ggcccaatct tcaaaatagt tcccttcttt aagtttttat ttttatgttt
     7801 taagtttaaa cctgattaca ttattgccgg ccccatgccc accaccacta ttctctacgc
     7861 tagattaatt cgattattta cccactctaa attaattatc aatgcttctt ttcaccctac
     7921 cgacccagaa tttcaccaac caatattgat aaatacccta aagtcagccg acttcgtctg
     7981 gacacttact gactttgaaa caaattattt tcataaaaac tttggtatcc ccaaatcaaa
     8041 gttaatcaat atcggcaacg gcattgactc atcgcttatt accaaatcac caactattgt
     8101 taaaaacaaa ttattattta ttggtagctt ttctgcccac aaaggtttag aaacattatt
     8161 tgctgctttt aaactactac ccaaaaaata ttctctaacc atcgccggta accctaccct
     8221 ccataaatta aacataccaa aaaacatcaa agttcttacc aacttcaagt caaagaaact
     8281 agcacacatc atagaccaat gctctatact catttctccc tctacccaag aaagctttgg
     8341 actagttctc cttgaggcca tggctcgagg taaacctgtc atcgctgcca acatcccagc
     8401 atctgccgaa ctagtcaaaa aatctaaagg aggtcttctt tttgaaccag gtaacccaca
     8461 agacctggct gcgaaaatct taaaaatttc tctagaccgt cacggtctat cttacgcaat
     8521 taatcataca tgggataaaa taggagagtc actatggcaa aaaatatcat cctagctttt
     8581 ctcatactat tactactagt cataatctac ttgatatttt tcaaccgacc tccagtcgaa
     8641 actctaaaga tagacatcaa aggcaagacc tacaatctgg agatagccaa aaccattccc
     8701 caaaaaatgc aaggtttaat gaaccgaaac catttgtgcc ctgactgtgg catgattttt
     8761 atatcaccca tagacactcc tcaaagcttt tggatgaaaa atacttttat tcctcttgat
     8821 atgatttttg tcaacttaaa gggagaggtg gttaatatac ttaccgctga ggctgaagcc
     8881 aaagacgaaa atggcgccta caaatttttc caaagctctt ctcctgcagc ttatgttatc
     8941 gagctcaacg ccggcgacgc ttcaaaatta aaacttatcc ctggagatgt catcaaactt
     9001 tagtctcact attccaaatt ataacggtgc cgcttttcta cccgactgtc ttgattcact
     9061 gattgtcgcc attaaaaact gccccgattc acaatttgaa ataatcctcg tagacaatgg
     9121 ctccaaagac gaaagtatcg ccatatttca aaaatatagt gaattgaaaa ttttaaattg
     9181 taaattaaaa attatttgtc atcagacaaa taagggtttt gctgggggag tcaaccctgg
     9241 gatcatcgct tcaaaatacc cctgggtagt tctggtcaac aacgacctaa ccgtatctcc
     9301 caactggttt aaaattgtca gtcaacaaat taccccagac attgccactt attatggcct
     9361 agtcctaact aaagacggca aacaaatcga gtcagaaggc ctcgactttc actactccgg
     9421 caaatgcaac aatattaaca accaccaaag ttttcataaa tcgtatttca taaatcataa
     9481 atcgtctttt atttggggtg ctcctgcatc tctcatcgtc tacaacaaaa atatactcgc
     9541 ctcaattggc aattttgacg aagatttctt cgcctacgaa gaagatgtcg acgtcgctct
     9601 ccgacttcat aaattaggcc acaaaacact ctacattcct tcggccatct cttatcatct
     9661 cggtagcggt actagtagta aaatgggtaa tttccgaaac agaatggatt ttaaaaactg
     9721 gatatatatc atcattaaaa acttttctaa aaaagaaatt attagcaatc ttccgactat
     9781 catcgaacaa cgtttacgca acttatctgg agttatcaaa aacaccccac ttctccaagt
     9841 accatccact atctttaaat tattctatga agtaattact cacatcccca ccatgctaaa
     9901 aaaacgccac caattccaaa agttactaaa acataattaa gtctacctcc acctgcgatg
     9961 tggatctctt ggttcattca caaaatcttt ttctcgacct aatggactta tcccgatacc
    10021 acccacagaa tccttcaatc tgccaaaagc acctttctcc aaaataaaat taatttttgg
    10081 atcatcacca aatctaagac ccttcacctc agcaaaagaa cgaacaggac ccaaagatga
    10141 atcactaaaa ccaagataat tatcaccatc aacaagagcg attttaatta aggtcgccat
    10201 agccggttca tacgccagag gtacatcaaa accacgcaat acatccttag ctttcattaa
    10261 actatacaag tccctccaag attcacctac tccattatgt ttacgtccct ctttaacaaa
    10321 tgacacgttt ccatcatcat ctggtctctg ccaacgagac aatttcgtct caaaagtata
    10381 agcgcctttc ttttttgtaa cacggtaata accagtatca accacacaat ctcccgagta
    10441 catcgacgcc attcttacag ctaccataaa tcttgcctca acaaacggaa tttttatctc
    10501 tttcttaaca ctatttacat aagttcggtc atacaagctc cttttatcaa catcagataa
    10561 agtcatatac gcatcattaa ggtcctgaaa tctagcttta gcgccaggct cactattgaa
    10621 atccggatga tattcccttg ccaactttct aaaagcactt tttatttctg tcaaagtagc
    10681 tcctcttgac acccctaata tttcataata gtcagaacta gttgtcatga catcaattct
    10741 attctagaaa ctgttaaaat caaacatatg attataggca tagacatttg ctcgattcct
    10801 tatggaactg gagtcagcaa ctatactctc aatcttgtcc gccatctagt tag
//
LOCUS       DDWP01000074            4590 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_59635, whole genome shotgun sequence.
ACCESSION   DDWP01000074 DDWP01000000
VERSION     DDWP01000074.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_59635"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1580 a          793 c         1002 g         1215 t
ORIGIN      
        1 aaatttgaat ggtaaaaaac ataaggaaga acaaagatgc ctgacccaac tgtaccgaca
       61 aattgaagaa aaatggcact agcaaaagtc gaggtcattt cttaagagtg ttgtcggtaa
      121 acgataaagg atactttaac ttctgaaaaa ccaaggtaat aacttgatta acaaaccaag
      181 taccaagagc taatatcaaa aagtaccaaa gaccaatcaa ggtcatctgt gggaaagact
      241 cctcactaag atagcccaat attccagata aaacaccgat agtagtgata agacgaagag
      301 agtcaatact attattggag ctacgagatt gaacttcatt aacgacttga atagcggtag
      361 aaatatagtc ttgagtcata ttccaaattt cttgaatata ggaatgagta tctactaggg
      421 tctcgtactt atattggaag agtgagagaa gatctttatt taaatttaat ttctcagaaa
      481 tagatgaacg agtgctgacg taagaactca tctggctaat acggcttttg atgaggttga
      541 tagttttctc gtaggaatcg agacgttgac gaacggcttc aacctcagca ccacgaataa
      601 aaccacgctc tttgatatca gaaatttctt cccaaatttg acgatgtaaa tcgagatagc
      661 gttgcagctg gtctttgaac tctcggaaga atatttgcat ttcaaccatc tctcgtaaat
      721 gagcttgaga agaagtggca gaaataaaaa tataattggg agttttgtaa actgagtaat
      781 cctttgatgt gatctgacta tagatatcac caagctccaa cccttttaaa tctcccttta
      841 tggtcgagat gacggtagga tgggtatttt ttatagcggc gaggacttta ggggtagggg
      901 ctccgagaga gaaaatataa ctaatggcag gatgaagaac gttgtcaaaa taactcttga
      961 gtttgttctc ggctgatttt atatcggaag aatcttgagt taaaacgtaa agaccatctt
     1021 cgtaataacg aatttcaatg ccagaagagg tgatggcttt gacatattca aaatccccca
     1081 tttcgcgggt aacggaagta aaaccaaaac tttgatgaaa agcttcaagc ttggatgggg
     1141 aaagatctag atttgatttg ccacgagcga gtagatcgta tatctcggaa agatggagag
     1201 tggtacgttg ataccaacca ccaaaagtaa ctgtgaataa actatttttc ttaaccatta
     1261 attaagaata gcaaattttt gattagtggt cgcggttgac aagaaactcg gggagatact
     1321 caatataatc acgagctggc tggtgagaca agaaacctaa ttcttgatga aaatattctt
     1381 gggcttggag ggcgaaagag cgcattaatt tgtcggcata atcgatacta ccaagagtat
     1441 tcatcttact gattatccag tcgacctctg attgggtttt ttgaaaacga gttttgtcta
     1501 gaattaccga aagctgtcgt ttgtcgtctc cagagatagt cctcataaga tgtgccaaca
     1561 taatcgttcg cttgccctcg aaaatatcgt taccggtttg tttttttaac ccacaaaaat
     1621 ctgaagtaag atctaaaaga tcgtctttaa tttggaaaca gtaaccagtg agcttgccaa
     1681 agtgatagat tttttcgagt tgatcgggag tggccccagc gattatggct ccttcgcgca
     1741 ttggaccagc aactgtatag taacctgtct ttccctcaag aattaagaga atatcttcat
     1801 cggttaaatt ttgagcattt tcttgagtcc attttatttc aactgtctgg ccaaaaacgg
     1861 tacggtttaa cattttggta aattcgtcca tgatagaaaa agttaactca tcgccaatca
     1921 actgacgatt gtcttgaagc aacttccaca tcaagacatg aacaatatca ccggcattaa
     1981 tagcgagctc tttggtatat agatgatgga gagctgaatc gccacgtctc tgctcggaat
     2041 cgtcttcaac gtcatcgtga attaatatcc attcctcaga caattgttga gcggcagcgg
     2101 ttagtaaaag cttttctcga ggggcaccca tggcctcacc agtaagcata accatggatg
     2161 gcctaaagta tttacccatc ctctggggat aaacactaag catctctaag tggaagtcaa
     2221 gcaaatgttg atatttttcc ttgacctgac aatatttttc aaaatcaata gttgtggaca
     2281 accttttatc aatctctggc caaacgaggg aacggtactc ttttaataaa ttgacaaaat
     2341 cttgatggac catagttagt tagtcgtaag aagataatta atcatttttt tctggagagg
     2401 gatcgggggt ggtctggcta tcggcatgag ataaaagccg atcgaaagta agcggtgaaa
     2461 ggaggtagat aacttcgaaa gtggcgacag aaagctcgaa agcgctgggg atctgactag
     2521 taacggcgta agcaccgata cctagtacaa gagcgttttg aaaactactg cgagtacgag
     2581 cttttataag acgttcaacg acctcaggtt caaccccatt ggcttttaga tttttggagg
     2641 cgatagaatt gtctttaagg ccggcaataa taactgtcaa aaacgtcccg atggcagtta
     2701 ttaaattcat ggtactgttc tgccgggtga gatcggcaac taaccaaaca aaagaaacat
     2761 ccgtgggaac gtgacgcaaa aaataactgg agacaatcat caaggttagt ccaacaccgc
     2821 cgatagcgat aaggatctta ttgtagccga tcctaaatct taaaattgat ttattgttag
     2881 ccaggaaaaa ctttaaaata cgccagataa agtggagaat agcaaagaag actgacagac
     2941 ctatccaaaa ataaataata gaaccccagg aaacggcagt ggtagcgttg acatggaaaa
     3001 aaggaaaatg aaaatcgcta cccatcagcg gatacgacgg gtagggaata aagtcataaa
     3061 ttaaagttgg atcaagagga gtgtggtaaa agttgattag aaaaaggcgt ccagcagaaa
     3121 agaaggcaat agcactaatt aagaaataga atatataaaa aataatggca aagtcccctt
     3181 ttcggacctt ctgttgaaaa agaatgtact gctcatcagt ggttttgatc tgatcaagct
     3241 cgtcctcgaa gtttttgata gaagtagaca acttgagact gatgaaacga agtgggagga
     3301 aagcaatgct ttgtaaaata ccaataagaa taactgatag ccaaatctgc cgggtgagat
     3361 agtacaagcc aaaaaaaata ctagcgtaaa gcctaataga tccgccacga aagaaccaaa
     3421 tgaaaaggag aattgaaaag atgacgataa aaaaagtagc caccggcatc caggctcgac
     3481 tgttgtcgat tttacgaact tggctaaaag cagaatcggg catatggaag tatacaataa
     3541 acatcaagct taaaacttaa aacacaaaac aaaaatagag aggagttggt ttatacttta
     3601 ttagtatgga taataaagag atactttcgt cgactcagga taaaaatgaa cagaaaaatt
     3661 tgttgctgga tgtagaggca agaactaagg ttccggagaa tataaaaaat tggctagaaa
     3721 agattgaagg cgatccggtg caaatgagaa cagtaaatga cgatagtggc cagccactgc
     3781 tacaaacatc tgccccgcaa gatccgagag ttgtcttacc aataaccaga cagaaatttg
     3841 tagctggttt taagaaaact atagatgagg ctggaaagtg gctatctacc tttatactaa
     3901 gactgattaa aattaaggaa ggtaatgtaa aatttaaaga agaatgacca tcgaatcact
     3961 gacattatgg gtgacacagg caatgctaat gctattgggg atagttgttt tagtagtatt
     4021 actaggtatt gttggatatg ggttgatatt gtggtataag tggaaagaca gagaaaaaag
     4081 atcacttgag ctgataactt taatggtagc ggtaccagaa gataatgagg taaaaataga
     4141 tgcgatggag cagattatta gctctctttc tagcctatac aagggtgcca agtttgattt
     4201 tttacagatt tttgaagtcc aacccagcgt ctgtttggag atatttggaa ctagtgaaga
     4261 tatacgattt tatataagta ttcccaaaaa atatcaggat ctaatcgaaa aacaaattta
     4321 tagcgtctat gctggagcag gaataactgt agttgatgaa ccgaacatat atccagaaga
     4381 aggcaaggta gagtattgct ggctgggaat taagaaaact cctttctatc cactcaaaac
     4441 ctacaaagaa attccaactg atccattagc agcggtaact tcagcactgt ctaagcttag
     4501 ccccggagaa agcgtagcga tacaactagt gttgtcaccc actaacggaa cttgggcaaa
     4561 gtcgggaaag gggtttgtct cggcgacaaa
//
LOCUS       DDWP01000075            2596 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_43409, whole genome shotgun sequence.
ACCESSION   DDWP01000075 DDWP01000000
VERSION     DDWP01000075.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_43409"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          865 a          447 c          545 g          739 t
ORIGIN      
        1 ctaataaagc agccgatgga tcaccgcgat agtcagcacc aatcttgtca acctcatcaa
       61 gcatgaaaac tggattcatt gacttagcat cttttaggcc tttaatgata cgaccaggca
      121 tagcaccaac ataagtgcgg cgatgaccac gaatctcggc ttcatcacga ataccaccaa
      181 gagaagctct tataaactcg cgaccgaggg ccttagcaat agatttaccg agagatgttt
      241 taccgacacc tggagggcca atgaaacaaa ggatattggc agtctgagcc tggtctttgc
      301 tttctttacc ttttagtttt aagacagcta aatattccag gattctttcc ttaacatctt
      361 taagaccata atgatcatcg tcgagaactt tctcagcttg aggaatactt agggcagatt
      421 ttgaatactt tccccaagga agttcagaaa ctatttctag gtaagtacgg atgtaactgg
      481 cttctggagc catagatccc atctcttcaa gacgattaag ctctttaaga actttttttc
      541 tgatctcggg aggcattggg gcttttttga gctttttttc aagctcacta gtttcaccgc
      601 caccttcttt accaagtttt tttagctctt tttcaatttg acgcttacgt tcctcaagaa
      661 cattgcgctt cataccgaca ttaaattttt cttgagtctt atcttcaata gctctctcta
      721 gctcagcaac tttcatctcc ttaattagat attcggaggc ggcgcgaaga cgaagggaaa
      781 caattaatgt ttctagcatc tgctgttttt ctgataactt agcgttaaca gaaaaacaaa
      841 cttgatcagc caagtcacta ctgctaacac cagtactgag ttgcatcatg gccggaagat
      901 caaattgtcg accaagggca aaggctttac gtaatttatc aagaaggacc tcagctgatt
      961 gttgaatgtc ggttaatggc tcggcaataa ctggcagatc tgtgtattcc accacatagt
     1021 gcggctctac ttgaaccaag gcagtaatat tgacacgaga aattccctta acaatggcgt
     1081 gtaaactacc gtcagtatga aggacatgct ctattttggc gagaacacca acattataga
     1141 gatctttgaa tgtggggttg tctacttttg aactacgctg ggcggtaacg ataacaaatt
     1201 tatcggtatt gtcgtaagcg tgcaaaagtg agttcattga ctctttacgg ccaaagaata
     1261 aagaagtttc gactgatgga aaaagaacca aattccttaa aggaattaat ggaagaattt
     1321 tcttgattgg ttcaagatct ttttgtggta tttcaggtag ctgaggggta gtaatttcag
     1381 acatatttag cagtctatca gatagtatgc tataaatcca cccctaatga ctccctcgac
     1441 tttgatgaag aatttatctg ttaaataggg tagaatactt atatatggat aatatcaaaa
     1501 aatttggaag agtggtgctg gtgggtagac caaacacggg caaatcgaca ctattgaatg
     1561 cattaatgaa tcaaaaggta gcaattactt ctccgctacc ccaaacaacc agaaaaagta
     1621 gtcaggtagt attttctgac gaacggggaa caatagtttt taccgatact ccaggagtta
     1681 tcaataaagt tgatgatttg atgggtaaga taattaataa tgaagcaccg aagtctgtag
     1741 ctggaaaaga aataattatc tgcctagtag atatatctcg accgaaaagt gaggaagaga
     1801 acagagtaat tggttttgtg agaaagtcac cagcaaaaaa ggtgctagtg tacaacaaac
     1861 ttgataaagc tcaggggcca aaagatcatt tggctgatta tcgatacctc gaagatgagt
     1921 ttgacaaagt aatctcagta agtgcaataa aacaaaaaaa tataaaaggg ctattgaact
     1981 tggtgtttga aatgctccct gaaatgagag agtcgaaaat aaaagaagaa ttggggccga
     2041 tcggagataa agatagacca ctaatgggaa tggggtcgag tgaatatgta gcagaaataa
     2101 ttagagaaaa gacctatcta ttcctcagag aggaagttcc ttacacgatt aaggtggaag
     2161 tagaaagtat taaagaaaag aaaaatataa ttgtagtaac ggcatcaata tatacaaatg
     2221 ctgatagata caagaaaatg attattggta gtggtggcaa gaagataaga gaaattggtt
     2281 ttaatgctag aaaagaatta gaactaatgt cggggaagaa ggtctacctc gaacttgatg
     2341 taaagataaa tagacactgg atggaaggcg aaagagaata agggtaaatt cacgtcttat
     2401 aatgttgaca aatgggcttg tttttggtat gatttacctg aacaatgcta cataattgct
     2461 atgtactttt tgttcaaaat gttctttaac aatcggtgga tagattagat ttcctactgg
     2521 ttgggtgaat aaataaaatg acatcctgtc attttttaat tcatccaact ggagaaaaaa
     2581 agaaatgaaa aagaat
//
LOCUS       DDWP01000076            7171 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_25897, whole genome shotgun sequence.
ACCESSION   DDWP01000076 DDWP01000000
VERSION     DDWP01000076.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_25897"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1624 a         1813 c         1112 g         2622 t
ORIGIN      
        1 gtcactgtca cttctttctt taaatagtca tctaaatcta ccttagtact cgtcatattc
       61 actatctcat cgttggtttt tagcagatat tcatcaccga tctttgtcgt caccactccg
      121 gtcttgctga ctattttttc ttgtattttt tcttcagctg ttttgctttg ttctgttgca
      181 gtgcagcctc ccagaaacaa cgctccaatt atcagtccca gaaatatttt tttcatattt
      241 ttagaattta gggtttagaa tttttaacaa ttactacctt gtgtctttct ttcagttctt
      301 taaaactatt taaccatacg gttacttctt ttgtggccgc agactcagtc gctgtagtaa
      361 ttgtcatttc tagagtcatt ggtgcgatat atttatccac tgtgaaccct tctcccttat
      421 acggcaacag tctccataag ggataatcgt catttggatt gatcgtcggt gttgcctcag
      481 ctgttggtac tgctgtcggt accattgtcg gtatcgcttt aagactttcg actttcgact
      541 ttcgactttc ttcgttttcc ccatatttaa tcttcactgt tcccataatc aaaattatta
      601 gaattagata cacaaatact tggtattttt tgagtttctc catcattctc ttgttgcggg
      661 tgttattaat attacttttg ctggccctgt cgctagctga gaattaccgt ttaaaaaata
      721 gaatggccag accacttttg atttaaagtc gtaaatatat cctagattat agtcatatat
      781 ttctactttt tctaactcca catcctctcc tgataattca tattcttgcc ctccatttat
      841 ttcccatatt tttatatctt ccgctctaac tttttttaaa ctatctaagc taattagttc
      901 cttttcttcg cttttaacca ctttttcaaa tggtatctgt ccttgtattt ttgccagttt
      961 tccgtttgta aaatagttag ctattattgg atggccatta aacatggcga ctggcacacc
     1021 ctcaaataca taattggctc tcagttctaa tgcgtcaaac ttatcccttg ttgccgatac
     1081 ccaccatggg tagagaaact ctttgtatct aatttcgaca cttcccatcc caattccatt
     1141 attaacctct tgaagttgct ccatcatatt ttctagcagt cccttgattg tcatatcatc
     1201 taaatcgccc tcttctattt catcggccgt ttctatgttt cggctaaatt catacctgtc
     1261 tgccgtatca tatattttga aaaactgccc cactggcatt gccccaccga ttaacacgtt
     1321 tcttatcttt gctttatcat cttcactcca gtttttacct tgaacgatca tcgccttagt
     1381 ttttttgtat tctttttcct catctatttt ccattcgatt tctttaggag ttttcattat
     1441 aaattcagcc tctggtctca ttagttttct ttcttttatt ggctcaccat tttcatcttt
     1501 tctaaaaaca ggccaaaaca atactagtag aaaaacaacc agtaatgttc ccagtattat
     1561 caatatcttt ttatttgtca tttttatttt gtgtaaggat tacacgatat tctgtcacaa
     1621 caccctgtca gattttttgt taatttaaac ttttgcatca aagaatcaga tatagatatt
     1681 ggcaatgaac ctcccctaat atcataatgt aaatggtttc ctgtagagct acctgtgttg
     1741 tttactgtcc ctattgcatc accagcctta acctcttttg tcgtccagtc aggaggagac
     1801 ggcatgtggg catatctagt agaaaagact gttccattgc attttccagt aacatccaca
     1861 taatatccat atattgcgtt tgatccggcc cttgcgattc cattgtgcgt ggctactatt
     1921 gtccccgtat agccaaaatc aaccgcattg gccatacttt tatgagagta ttttgcacaa
     1981 gacggtccct gggtaatatt ggttaatatt gatttggcat caccaattaa tggtaatcca
     2041 tctggacaac catcaggtat tgttttcggg tctagtggtg gtaatggcac atcaccgaca
     2101 atttctccag tactctccgc cattacttca cacaaatatc tgtccccatc gtctacgacc
     2161 actactaggt ctggtccaga ttctgatccg cttcctcccg acccttcgtt tccggaccca
     2221 ccatcaaagt taatggaatc aggtaatggt gccgaggata tccaccccca agttgacctt
     2281 agctcttcga cataagctct tccagttgtc ccgtcgacca cttcatcagg gtcacatctt
     2341 ccacttaagt aattcgttga aaatgcaagc caatagtctt gttgagctag tgccggacat
     2401 cccgaagctg gatcaagttt taaaaactct tctatctgag cgttaaaatc ttcataaggt
     2461 tcatatatta ttccaaagtc ggccaactca acactaaaac catcataatt acttgcccca
     2521 gattcgtgta accatgccca cagtgcataa tccggatttt ttccggcttc tttcgccttt
     2581 tttactacat cattaaaaca caactcagca ttttttcctc cgccagttct ccatctttcc
     2641 gctacactaa taaagttttg tttatcgaca tttgtacccg tctggtctgg cacagtcgtg
     2701 tcacaattta tgtctccacc tggcacgacc tcctcctcag gtccaatata gatacaataa
     2761 ccaccaccga cgtacctgtt ggctacccaa ggtaaaattt gatttaaagt tatagagcta
     2821 gatcctgcca ccatcgacaa tccaaacaca attactgcca ccgccgcccc agtaagtatt
     2881 aatcttgcct gcataaaggc aaagaaagca aatagtgccc ctattccgta caccgctagt
     2941 tccattcctc cacccaccaa ccccccaaat ttgtctttta gacccagttc tcccaggtcc
     3001 caattcattt tcttcaggaa accgtctatc gccgcttttc ctacttttat tatcttccct
     3061 ccagcccaca ttaatgcctg agcagcaatt attactagcg ccactaccca acccaccacc
     3121 gggactgccg aagctgcacc ggccgctgtt actgccgcgg ttgaccctgc tgctgtagtt
     3181 gccgctgctg tcgctcctgc ttttgccgcg gttgccgtag ctacctttcc tgtcgccaga
     3241 atgctcttta gggctatcat tgtcccttct tttagtccat gagtggcaat ctgtgaagcc
     3301 attgccgctg ccatttccgc tgttgtttgc gttccaaaac ttgcagcaaa tttttccatc
     3361 cccggcacca tttttccaac caatcttaac gggttaagag cattgattct tgcctgtata
     3421 ccagcaattt tctgtactgc ccctatcgac tgaaattgtc caccaagttg atttgccgtt
     3481 ttgcttagtt gacttacggc tttcactttt cccccaagac tcgcatcaag ttcagccagc
     3541 tttccaggca gttttgctac atttcgtagt ccacccttaa ttgaccgtgt caccatcttt
     3601 gcctcttgtg ccacttctgt tacttgcctt tcggtaaaac catttttaac cattgttcct
     3661 ctcatttctt cgtggtctcg ttttgtcact acaagctccc tcactatcct tacttctcct
     3721 aggttagccc catgtcgtaa catttctcct cttatctccc tcagttcttt cgctgacgag
     3781 ctattcctta attcactaat ctcgtttttt actgcttttt ccaatatcga cagtttttga
     3841 actttttcct ggcttatcgg tctgcctttt cttgttgctt tgttttcgta gatcggtgta
     3901 gcttcctcat cgttaactgt cactgttatt tttccagtgc tgtcttcagt tatttttatt
     3961 ggattcttat tatcatccgc ttcgttccat ggtcttgcta cttcgtcttc tatttctctt
     4021 ctaacacttc ttttcaattc tgtctgttcc gtttccgtaa cttctccaat tatctctctg
     4081 tatctcgctg tagtttctct aaccaaagtg tcaacctgct cttcaaccct cctctgatca
     4141 gcaacctctt ttttcaataa ttcaacctct tcccttatct cttgtgcctc ttctttgctt
     4201 tttttttgcc caagttcctc acctctaatc tctatcggct tttcctcaac tattgcttca
     4261 actactttgt ctactacctg ttcatccgcc tcttttccga caatcagttt aacttccttt
     4321 tttatttcta ctttttcaat gactggccac aactctttta ctgcttgttc tttttgttgt
     4381 gcagttaatc tcttttgtcc ttgaataaaa tcttcaacag cctttttccc tcgtggagct
     4441 ccatcttgtt ttaacgattc tttgtcccaa gcttccaaca aactctctag ttcttcactt
     4501 gttagagcct tcgactccat cttgatctca tctaaagcga tttcaacctt ttcttcagct
     4561 ttaatttccg gttcaatttc ctgtaaccta ctaattatcg cttcacggcc ttcttttttc
     4621 agatcgtccc agtctttacc caaaatgtct tgacacacct tttgtagttc tagattgttt
     4681 tcaaaatcgg tcgtcggatc tcttaatatc gctaatacat caccaatcaa atagatttct
     4741 tttgccgtga tatttgtccg tgccatcacc aaattatatc agcttagact atcccgtttc
     4801 caaacagttt gtcataatcc attttaggtt tttcttcttc tttttttacc tgatcatcat
     4861 ttgagttttg agttttaatt tttgagtttt cttcaaaaac tggtttattt ttctgaaatt
     4921 gtggtggttg aggtattttc acctcttccg tctttggcat gaaaatgggt ttattagcca
     4981 ctgatggctt tggtgcttga ggtgttacca cctctgtttt catcatttta aatggattac
     5041 tgtcagagtc tcccttgtca aagggagaag ccgccgtagg cggagaggga ttggattcag
     5101 cctttggcat cgccggtagt ggtggcagtg gaggcagttt gtttttattt acaaacaaat
     5161 cttgatattt tggtagtcct cctgagagcg atccaaagtt acctagtgtc cctgccaact
     5221 ttttcttttc ttcttctttg gccgtcttct cttcttcctc tatcttcatt ttttctttat
     5281 ggtcttctac ctttgtcttt tccagctctt ccatttccgc aattcttttt tgcaatatct
     5341 cttgcgggct catctccaca acactatctc ccttgcttgg ctcaaccgaa ggggtcacca
     5401 ttatttctgg cgcaactact tcttcttcta cctctttcgt taaaggaggt gttagagatg
     5461 gattttgaac atcagtgttt cccccttttt caagggggga ggtcacaact tcagttgtga
     5521 caggggggtt taaaacagca gaatcctttt tctcttccgt ttgtaatttg gtatttggtg
     5581 attggtcatt gtttggagca cttggtattt ggtgattggt tattgcctta aacaccggta
     5641 cttccacctc aacctccgca ctcttcatct ctgctctttt agctgcttct gcttcttttt
     5701 tagctctcag cacctcttct ggatttgatg taattaattt atgttcttct tctgatgcca
     5761 ccactctaat tgccacatga ttttgtccag caaaaaatat tccctcacct ttatcggctg
     5821 ttagtagcaa ttgtttttct ccttcagaca gataaaaaac ctctccaatc tgatcaattg
     5881 ctgcactatg ttgtttaagt aatatttgaa tcgagctgtt agtaacgatt tctctaccgt
     5941 aagcagtttc tagaaaatca tcaacatcct gggtaattgt cgtcactcca agataatatt
     6001 ttctacctct tttgactatc cccctcaaaa acgcagccga gtctggaaat tgcatcatgt
     6061 accaagcctc gtccactacc aatatccttc gtcgcaattc ctgtttaact actgtccaaa
     6121 tataatccaa gattatgtgc atggctactg gccgaaggct atcttccaag ttccttattc
     6181 caaacaccac aaactcattt ttaagatcaa ccgtcgtctt ttgattaaat attccggcaa
     6241 aagatccctt gatatatttc tccaatcttg ctgcaatact ctgagctttc tgatcttcca
     6301 ttccgattaa cgatttgtac aaatcttcta ccagtggcgg ctcttttgtc tgtgtttccg
     6361 ggtcaggagt tatccctttc atcttataag catcgaccac tgccctatca agaattgact
     6421 cttcaattgg atctaattct cccaacatta tttttagtag tttatgcaag ctcaagacct
     6481 tagaattcaa agcattttct ccttcgtcta ccaccagaga taggtcaaac ggatttattt
     6541 tcgaatcttc tccatatcca aaagcaatat actggccacc caccgattcc gacaaactcc
     6601 tgtattcatt ttcagggtca attacaatta tttcagttcc catcattaga gatctcatta
     6661 cctccagctt aatcatgtac gatttacccg ctccggccgt cgcaaacact accgagttgt
     6721 agtttggcat tgagaatcta tcaaaaatca ccaacgatcc attatgttcg tttattccat
     6781 acattattcc cttatcatcc gacaggtcag aagagacaaa cgggaaagtt gtcgccagtg
     6841 aggttgtgtc catgttcctg gttagattca gccgatcttg tcctaagggt agggtagtta
     6901 tgaaagcgtt ttctgaatca taattggtct tcttggtcag caccatcaac gctcccattg
     6961 ctgactctag gtttttggtt attctatcca agtctgctct cgaatcagct ccgatagtta
     7021 cgtatagccc catctggaaa aatctttcct cccccttcac taagtcggcc tgtaagttga
     7081 gtgcatcttc aagtttggcc tgtgttgctg ggttaactat cctacccctc tgtatatcag
     7141 tagttagttc ggcttccatc tcggtaattt t
//
LOCUS       DDWP01000077            3796 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_58050, whole genome shotgun sequence.
ACCESSION   DDWP01000077 DDWP01000000
VERSION     DDWP01000077.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_58050"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1232 a          661 c          834 g         1069 t
ORIGIN      
        1 ggggtaactt tgacggcacc agtaccaaac ttggggtcga ctgactcttc accaataaca
       61 ggaatttcac gatcgacgag aggtaatttg atggtggtac caataagatg tttgtagcga
      121 gagtcacctg gatttacggc tactgcagta tcccctaaca tagtttctgg acgggtggtg
      181 gcgacttgaa gaggtccgta attgataaac caaagatgag aatcaacgga tttgtgaatg
      241 acttctaaat tggaaaaagc agtgccacaa aaagtacaat aattgactat tttctcatca
      301 cgatagacaa gaccatcgtc acacattttt ttaaagacag caaagacacg cttgttgtgg
      361 gcttcatcaa gagtaaaacg ttcacgagtc cagtcaagag aaaaaccgag acgcttcatt
      421 tgagacttgg cgatggttcg attctcattt acataatcag caattagatt gtagagagtg
      481 acagaatcgt aatcgaagcg ggatttgcct tcggctttaa gatgtttttc aaaaacaaat
      541 tgagtctcaa taccagcgtg atcagttcct ggaagccata gagtgggatc acctaacatt
      601 cggtgaaaac gaaccataat atcttcaaca gtaaagaggg cgtggccagc atgaagaggc
      661 tcgttggcat ttggaggagg gaggatgata gtaaaaggct ctttggtacg atctattttt
      721 ggggtaaaag ctcccgactc ttcccaaaac ttataaatat tttcttctga taagggtgaa
      781 tatgttttgt ccattatagt ctcctacgac aaattaataa ttgtttatag ggtgccggat
      841 ttttaccaat tcgacttttg gcggggtcta ctattgtttt taatatgaga cgccatgcat
      901 ggcgtctcta caaaaaacgg gttctgcccg tatctccgga gtgactggct ccatcagcga
      961 tatggagtag tataacagaa aaactaaagc tgggcgacga attcaaaggc tttgtaattc
     1021 caatttattt tctcttctgg actgacttta cccggagctt cggtagtaac agctttaaaa
     1081 caacctaaag aacggatgaa tttctcagtg ctatcgcccc attgatcgtg attgtcttca
     1141 acaacagtcg ggcaaccaat atgggaagct acttgatcgg caaaattgtg agaatcggta
     1201 cttaatttgt agacaaaacc atcttcatcg atatcctcat ggaggtcaac aaatagatca
     1261 atctcttttg gagaaagata atcaattatt tggcgaacat gagaaggacc actggaattg
     1321 aaattacgat ttaaatctat gccggtgtga tgacggtctt ttttacccca accatcttca
     1381 ttaataagag gcaaaataat tgtctgatag ttgtcggtat ttaatttttt gttttggaga
     1441 tactttagga tagctaaagg acctcccctc tcgtcaccat gaacaccgct cataatgacc
     1501 ttagtttttt tgcgagattt tgggtttatt gaagcaatta tagaaccatt atcaagcgcc
     1561 acaatatcgg cacccaaaga agtagaaaca tcgacaaaat ggtaattaaa ctcggaaaag
     1621 ctgagcatag aatcgactat tgtactagaa aatttgatta ttggcaattc ttggctttga
     1681 taaaaccagc ctttattgct tcggcttcag tgcaaaacca gcgatcactt ttctcactag
     1741 aatttttttt tggtctcatc tcggtagtgt tgtaagaacg acaaccagga acagtgtaaa
     1801 tataatgttt ctctttgtag ttacctttga tattacactt aggattaata gggttgacag
     1861 tttgaagaca tttctcttga aatataccta tcttttttga cttattatca gcattaactt
     1921 gcttgagaat ttcataatcg gggtaactac cagaagtgta gatggcaaga ctagatttaa
     1981 tcatctctaa attaatatat ttgtcttcaa gatagacttg tgacaaaact cgaccatctg
     2041 gggaaggggt gtcactagta atcttgactc gtttattcaa aattaaatta gttaaaaaat
     2101 ctcttgattc ttgatagaga cattcattct gatcgggagc atcaatgttg gtaagacgaa
     2161 catgaaataa attatcaacc acaaaactat caccatcgta gacctgggtg actaagtaag
     2221 tggggttgta acggtcgggg aaacgattga aaagctgagt atataaaaat ataaacagat
     2281 cgaacataaa aaagaataac attttatttt caatccctct aaatctccct ttgacaaggg
     2341 agactgggaa tggggtaaaa taggcgcatg gataaggggg aaatacaaaa agctgcaagc
     2401 gttataaaaa aaggtggatt ggtggtgtac ccaagtgata cggtatatat cttggctgta
     2461 gatccgaata atgaaacggc agttaaaaaa cttttggctt ttaagaatcg ttgggtgggt
     2521 aaagcaatat caatcgcggt attggataaa aaaatggcgg aggattatgt gactctatcc
     2581 cctaatgcag aaaatattta tgcaaattta ttacccggac catttacaat agtgtcggag
     2641 ggaaaacatg tcgttgcccc agggatagaa gctgaaaacg ggacattggg aattagaatt
     2701 cctgataata aaaacattca cgatttggtc gaggcagtgg gtggtccagt aacggcaacg
     2761 tcggctaatc tgtcggggag gacaccaaat tattcgatac agagtttcct ccgaccattg
     2821 tcaaaaaaga aattggtgat gatcgatatg atagtcgacg ctggcaagct tccaaggaac
     2881 aagccatcga cggtaatcga tgcgacggaa agtgaggtaa aagtgctgcg tcgaggtgac
     2941 ctggtaacaa ccaatgcgac aacaataatt tctaagtcgg aaaaagaaac ggaaaagatt
     3001 gcagagttct tgctgaataa acacaaaaaa caaaaaacaa aaccgttagt atttggattg
     3061 acgggagact tgggatgcgg aaagacagta ttctcgagaa aggttggaca tttacttggg
     3121 gtggaagaaa aaataactag tccgacattc attatctaca atgagtattc aatctcccct
     3181 aacccctctt tgacaagagg gggacaagac atcctaaagt ttttgcattt tgatttgtac
     3241 cgacttagtg gaaaacatga gtttgaggaa attgatttta taaatcaatt ttccccttcg
     3301 acaggctcag ggctagtagc ctgtgtggag tggccagaga atatgggtga agaaaacttt
     3361 aaaaagctaa aagagaaaac taattatgtg gcaatagagt ttagatacgt ggatgtagga
     3421 acaagagaaa ttcgcttcta aatcaatttg aagtcagaaa gaagttggtc gcactcagat
     3481 ttttgagcag aatcgccacc atgaacacac tgaatagtat atttggtttt atccacttga
     3541 aaatcgacaa ttgtctggga agtgatcttg tacacaaatg ttggttgatc tgaaacttta
     3601 aagtcgctag taaaagatcg gcctggatga gaccaggacc aagttggtcc aacgtggata
     3661 gctatacggc ctgatgatga ataaagagta tatcgagtgt tgctaccgga ataggtcttg
     3721 tacagctctc tattgggaga atacttaacc ataaaactat cggtagttaa aataccatta
     3781 gatatctgct cggatt
//
LOCUS       DDWP01000078            6162 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_10599, whole genome shotgun sequence.
ACCESSION   DDWP01000078 DDWP01000000
VERSION     DDWP01000078.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_10599"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1924 a         1341 c          996 g         1901 t
ORIGIN      
        1 caccaatacc agcactgtta tcaaaccaag aaaaattgtt tttttggtta tttcgtcctc
       61 agctttctta tctaatctag agcgagtaaa ttctgccata aactatatct attctaacat
      121 tttttgcgac atcattcagc caccaattaa ttttatccaa aacttcagac aatcaggagc
      181 cgaaggggag aatcggactc ccgacccacg ctttacgaaa gcgttgctct accaactgag
      241 ctacttcggc aaacatattc caatcctatt ttacaatcaa acgccttcaa agactatctt
      301 gagaaaataa atccacttct atttcttgaa gtatttggca aacagcataa agatagtaaa
      361 aaataattcg gcacaatcat tccgtaattc gtagctttat tgaaaagtca tcatcaattt
      421 tgatgatcaa agcaataaac aaccttagtg ttatgagata tgtttggtaa tgtttattct
      481 catccttctt gttatctcca gacatagatc catgtaggta aatattgcga aggtcatgcc
      541 cattactcga atcttgctga ttgagataga aactaaaata ctttgcttct ggatcagtaa
      601 gaagcgaact cttcattact aaccatcctt tactaaccat agcatctatt tcagctcttc
      661 cctcagcaga gtagtgatag tagctcaatg cttcggcttg gaacagattt tttagtataa
      721 gtatttgatg ctggctttga aatattaaac gtttcgatgt tttcctgaga ataccccact
      781 taatgagctc atctagagat tgcttctggt aatcgtgaaa atcactataa ttaacctcat
      841 gattgattag taatctcgca aagtcctcgt ccttaagttt gtcatcaata taagttagcg
      901 ccgattgatc agagaacaaa taatgcatta ttgtaaagat cacagggctt tcggttgcat
      961 atgcgtattt accagcaacc tgactaggag tgtctttgta ttttggcgat tcggatgtaa
     1021 ttgacaacag gtcttggtct atcaatccat tttcagtgta cagtttaaat tgtttaatga
     1081 cgctatccat ctcactaaaa atgtgtttac acttttcaag atacgtagta cccttacttg
     1141 aaacagtata gttgaatcca tcggcattga acgttttttt cagatattcg ttgaagaacc
     1201 acccaatcac atcttcgagc tccttatttt ctgaccgcaa gaacttctca tacatcattg
     1261 tctgcataag tgtactttga tcaataaact ggaaatgaaa accctctggg tattcgcccc
     1321 gtccagaaac tttcataaaa cgttcaacaa cactcaactg tgacgaatat gatggtaatg
     1381 tcaaaattat gtgtttatta actagcggga aaagatgtat gaagttgctt aaggccgata
     1441 aattatctga atgttcctta agccattttt tactgtaagt gaattttgtt gttacatcac
     1501 cttgtaatat tttaactgcc tcttcctggg tttcagatat tgctacttca gtaccaaata
     1561 tgacacgacc actattctct ttgaaaaagt tgtcgttcca ctctgcgtgc ttccgttttg
     1621 caagtaattt aatcttatcg tctataccgg cactaggcat aactcttgcg ctagcaatca
     1681 actggaggta attgggattg gcacctgtac tgtttatgta gctctccaga agagtacgag
     1741 tatcgtcttt agtgaaactt ggtggtaaat gaacctcccg acgctctttt ttctcaagat
     1801 acttgtggat taactgttct gcattgtctg ggtcggataa aattaatgtc cgtatatttt
     1861 gatcgtattt attaacaatg tctttgtttg ccaataatct ccatatagcc acctttgaag
     1921 atcttaatgc tcgcaacatt acatctgagt ttatgctgtc gtaacttttg tagcgagcca
     1981 ggagaattag cagatcatcg tgatagtcaa actttacttc tgaaattatg gttaacgcgt
     2041 tctcttcctt aactgcatta aaaaaagctg cgattgtttt atatagtttt ggtttgagag
     2101 taatatattc atctaacttc ttcttctcaa tgtttttcgg caaggctaga ttctcaacgt
     2161 acaaaagaac attgtacagc tccagaatat cattaatact gtaatccacc ttagtctcat
     2221 taaagttgtc gaggaatttc atagtaggct ttaaatagaa agaagtagcc aaatcacgga
     2281 tgccgtaata ctttattcgt cgctctgatt tctcattctc ctccaaactt gaatatttaa
     2341 cataaaggtc atcaattttc attaataatg ccttgcgagt aggagttgac ttgccgttta
     2401 accaattatt gagcgatact agagaagtac cgattttttc agcgagagac tgctgagata
     2461 ggccactggc agtgataatt tcttgcaact ttgttctgta atccataaac aaatcataca
     2521 taactttata aagttagtaa agactttatt aaagttatgt tttcaaaatg agttgccgcc
     2581 gtcatatcta aacattaaaa aaccaagacc accatgtttc ctcaccatcc atcccgtata
     2641 atcttgttca tccaatcagt tcatttaaat cctcaaaaag tggtccgact ccggttgccg
     2701 gtaaacattt ttctaaccta aatacaaaac gttaaaagta gtgtctagaa ctcagaaaaa
     2761 gattgatttc ttgaggaaga ttggagaaat acaaaaccat ttcgctattg agcgggtgat
     2821 gggaatcgga cccacttctc atccttggga aggacgcatt ctaccggtga actacacccg
     2881 cttcaaaact attctatcac gctagttagt tcagcttggg caacaccaag gtcattccaa
     2941 tctccaattt atctgggcta gataatttac ttttattatt ttgccagatc attacccatt
     3001 tatatccatc accatattta ttaactgcaa tgttccacaa gctatcgcca cggacaactg
     3061 tataagtact ttcgtcaaca gaaacaccct taccatcttg gactttttct tcagctacag
     3121 tatctttctt taccggctca gccttagctc ttagctttat tttttgaccg acttcaagag
     3181 tagttgtttt taattcatta tctttaacga tttctgtcca tctaaatcca tcaccataat
     3241 tagctaccgc tatgttccat aaactatcac ccttcttaac cacatattca ccttcaacta
     3301 cagccttatc accagtagtt ttattgccct ctaataccaa gtcactattt actccaggga
     3361 tagtaatctc tccacgtctt ttctgaaaga aattaaaaat cattcccacg acaacgacta
     3421 caataaccaa ccccaaaaac atacttatgg tttcctcatt tagttttatt ttcatatccc
     3481 taacatacat gaggcttggc ctatagtcaa cgacgacttc agcttattct ccgcacaatc
     3541 acatcatcca aaacaaacct atttaattct attacgaaat tatttaaaac ttccttgtct
     3601 aatttataga ctctcaactt acctcttact tcaaaactaa ccaacttggc ttttctcaaa
     3661 atcgccaaat gactagacaa cgttgcctgt tttattgtta aatatttcaa gatctcattg
     3721 acagttagat ctccattcct taatatagtc aagatcttac gtcggttgat atccgacata
     3781 catttaaata ttttatccat agtacgatta tatcgtcaaa catcaatatg aacaactacg
     3841 atgataaaca agaacaaacc ctagttatag tcaagccaga cggtgtccaa agaggtctaa
     3901 ttggcgaagt catcaagcgc tacgaacgat gtggtcttaa gctcgttgct cttaaaatgc
     3961 ttatccctac cccagaagtt gccctagctc attattccac tgatccagaa tgggccatca
     4021 ctactggtac caaagcactt gcctttgcca aagaaagagg agagaaactc tttactgacg
     4081 atccaattga atatgcagag aatattagaa aaaatgccaa ggtcttttta tcttcaggtc
     4141 ctgtcgtacc tatggtctgg caaggaatga acgccgccaa ggtcgtccgc aaaattactg
     4201 gtgttaccga ctgtaccatg tctgtacctg gcacaattcg tggtgactat gctctagact
     4261 cttacaccac ttccgatgct gacaatagat ctatcagaaa tataattcac gctagcggct
     4321 cagctgaaga agccaggcta gaaattccaa tttggtttca accaaacgag atattgaact
     4381 atcgctacgt ccctgaagcc atcatgtacg acatcaacct tgatggaatt ttggaataga
     4441 gattctctcg attaaaacta aaatccgcca atcacttggc ggatttttta taatcattta
     4501 tcaaatgata ttatctacat atgaaaatct acccaaaaat gaacaagagt aatactgctc
     4561 ttttggtcat cgatccagtt aactcttgcg ctcacgaaaa ttgcgagaca ccagagtgga
     4621 acatccactt caccaaaatc agaaaaatgt tacccaaact agatctattc atcaaagaat
     4681 atcgtcaaaa aattggtggg cttgtagtcc taaccactat caccccgtgg aacaaagaaa
     4741 atctcaccga caacatcaat gaactttaca ccgaccctgc tgccacctat tactccgaag
     4801 acacaagtgg ttttgacgaa caatttcaca ctatcaaacc agaaccaacc gacctaattt
     4861 ttaccaagaa cacttacgac actttttccg acgacaaatt aacccaagaa ctaaaaagta
     4921 ggagagtgca atacataatc gtcaccggaa tatttactga cggttgcgtc ttagcatcta
     4981 tcgttagtgg tttctctaaa ggctttaatt ttataatcct aaaggattta gtcgaaacca
     5041 ctgattcacc aactagacaa gaaattcaaa aattactctt cgagttcacc tttccggcca
     5101 tgtacggcaa aacaatctct tctgaagaat tactccaaga gcccagttta gtaaccaaat
     5161 aaatgaaaaa cataattttt atcactggca acgaatacaa atatgtacaa gccaaggagg
     5221 cgttaaaaga aaccgacatc aagctaattc gccaagatat agacacacca gaaatccaaa
     5281 gtacaaaagt cgaagacatc gcctcgtatt catcaaaatg ggctagtaca aaactaaacc
     5341 aaccagttgt tctaactgac gcaggatatt attttgaagc cctaaacggc ttccccggac
     5401 ctttcattaa atacattaac caatggttca ctgctcaaga ctatctgaat cttatggcag
     5461 gaaagaccaa tagaagagta atcatcactc agtgcctgtc ctactgtgaa ccaggacaag
     5521 atccggtaaa cttccttggt tctgtagaag gaactatcgc caattacgtt ggttctattg
     5581 gtaagtggaa gacaccaata aaccaagtct atataccaca gggttacgac aaggtagtag
     5641 gggagctacc gtttgaagaa atgatgaaag actggaacag cgacaccgtc tggtctcaat
     5701 ttgtagagta tttaaaaagc aaaaaaagat agctatttat tctgattgta tggattgcac
     5761 ccaatcactt ttatccttag caaattctat ttgacatcca acttcccata gtttttgtgc
     5821 aataactggt ccaacggttg aactaattgg aacctccttt ttggatgtaa aatcagttat
     5881 acctgccact actgggtaca ttccactaaa aagtagttct gctggaacac ctgcagccaa
     5941 ttcaccagct gaagtcgcag ttgccgtccc aaccaaaagc atacatgctg cctcaggact
     6001 cattgttttc tttgaacctc tattaatttc aacagcaagt ttctgaattg gattccagtt
     6061 tggattatta acctcaactt tcggggctat tggtgcagtg tctaaagtca tagcaaatta
     6121 tacatgattc tacgtttgcg ggtcctatgg gattcgaacc ca
//
LOCUS       DDWP01000079            5484 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_21802, whole genome shotgun sequence.
ACCESSION   DDWP01000079 DDWP01000000
VERSION     DDWP01000079.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_21802"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    5091..5129
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1610 a         1152 c          848 g         1835 t
ORIGIN      
        1 cagctagagc tctaatttgt tgccaagttc tttccatgac cacactcgtt caatccctgc
       61 cgggacctct tcatcagcgt tatacggcca agaaaataaa tataaatttg gtactagtcc
      121 aattaaaggt attagctttt tcagactgtc atcaataaag tactcaaatc cttctagtgc
      181 ctgggttttt tcttgtctat tagtaccgac taccccaatg tcttttaatc cattaatttc
      241 acaccacctt tttgccaaac ctacccctat ttgtgtcctc gatgtaatta tagttatatc
      301 aaagctttgt cttaagactc cgatcgtttc aactgcccca gttaccggtt tagtttccat
      361 tactatttct gattcataca tcaataccgc taactcctga tattgatcat tagttataaa
      421 tccgtttttc atcacagctt cgtatccagt caggtccaga ggtattttta ctccaaactt
      481 ctcttggaca atttgctgtt taaccgctct cgaatcagat attactccat caaagtcaat
      541 tccaaattta ttcttcatct gtctatagtt taatcacatt caatgcctgg agtctaccaa
      601 aaatagacag caagaatccg tcatttgtat ctgctagaaa acttaataac tcagatattg
      661 gtttaataga atagctaaag ctataacctt tatcatcata tctttttcca tcaatgtacg
      721 tctttataat ctctacattc atgtcattaa ttagtgcagc aaacagacct actacattgt
      781 ctgacattgc cggatctggg taaataaaac caagatcaaa atattttgaa aaattgaatg
      841 aaggaatcct catttgctgg gcaaattgtt gttcgacata cgctattgct ccaaattgtt
      901 cgtcaccaaa tgattttggt ggataaatgc ttcccatagc ttcttcaatt tctctgctaa
      961 ttgcaaatct ggtgcatctc cttagtataa agtgagatat ctgattgtcg gtttccaata
     1021 aaattattcc acgcaggcta tggtatgtcc ctttcttcca accagtatag actttttgac
     1081 ctattttatt gttaaataat gacttaactt tgaatacaac gtgggcaaaa tgctcattta
     1141 tagacacatc ttccatctcc aatatttcaa aatctacgaa cttttccgga cggtcacata
     1201 aatcattcag gaaaccctca aagctctccg gtaaatttac caattcatcc tgtatttctt
     1261 ttttagaatt agcaaaccat tctagactat tcaatttact tattgcttcc gcccttgtgt
     1321 aattgtttgc tgaacaaatt attctattat ccatgattat tgatttctat aatatttaat
     1381 gccattaatt ttgaaaaaat cgctaataga tagctgtctg caattttatt aatatatgaa
     1441 cgggtcattt caacagattc gacctccaaa ccatcagttt gattttttaa gtcaacacta
     1501 tcacctttta ttattatggc aaaagttgag ctatgattat ttgttaaagt ggtgtcacta
     1561 tatacttctc ctaaatcaac gaacctagtt atttccaact ctcttttttc aagcattttt
     1621 ttcagttgag tttcaatttt tttaggtaaa gtaattaact ttggattatt gaattttgga
     1681 tagtgcatcc tgattgcttt gctgattagc gttccagtta cgaagtcgac cgttcttgtg
     1741 gttacaaaat actttatttt gcccatttct tcaatcaaga ttattccatt acattctaag
     1801 ctaactccag tatcatagct tacatattcc cgaagcattt tttcattatt aagttttgac
     1861 tcaacctcaa aacttagaat cgccattttt tgggaaatca ccatgtcatg taatcgcaat
     1921 attgccttgt ctcctgcata gtctggccta ttagacaact ccttgagcat accattgtaa
     1981 ctgttcggta actctaatat ctttttctga atatcaaagc gagtttcctt aaaccaattt
     2041 ttcaacaaca gtttttcaat caatatttct ttactcggat caattttttc tagtaatcca
     2101 attaactttg tttgcattaa ttattctaac acttgtatcc tataaagtga aatatagcat
     2161 tacatcactt aaatacattt aattaaatat attataatca attgtgctct caaacaatat
     2221 agacattcat aggcctcagc cagaatctag tccatcgtta cctcgtgctt ttctggaata
     2281 tcagaacatc gccgaactca aggcgcttgt tccactaaaa gaacaaaatc caaaaaatgt
     2341 tgaagttatt cgaggagagg agctttctag agttattcaa gacttagaga ccgaaccaga
     2401 cccctccggg cttatctcag atagtcagac aggtaatacg cacagacctt acgccaatgc
     2461 tgagataact gtcgaaaaaa acaccagttt ttctgacaag aaattgactc aaacctactt
     2521 actagaacat cggctaaaca acttaacgta cttagctatg ttggctcgcg aattcggtgt
     2581 ggatatgttt cacttggacg gtgtcactac tctcaaggac gaccagggtc aaataatcgc
     2641 caagatatct ccaccagttt ttgaggttaa ctcgcaagga gaatataagt tagttgccgg
     2701 acatcaacga tattttctag cttatctttt aggcgaagat gacattaaca taataactat
     2761 tagaaatttt gatccagatc atgccaggcc atatgaacca gtagatttta atgatgttaa
     2821 aattcttaat tctcctccac cagtagatag acgtcgcaca ttaatggcgg gtcattctga
     2881 atatgatttt gttgacttta ctgcgctggg cgaagatggt aagagaagaa gtcgtcttga
     2941 attaggtaaa ccctatttcc ccgaagaccc cacaaaaggc acttcaaaag ccatcaccaa
     3001 gcaggtgaat gccaaattac accaccttgg agcgggtgga tttttcccct cagctattaa
     3061 tcgtagtgca gaaggtctat cacgcattcc tgaagtccga gatgctattc ccattgatga
     3121 caacttcttt cttgccgata tcgatattga atggcttaat caagataggg ttgaattaac
     3181 ctctcgctat caattaatta ccgaatctaa ggaaaggtcg aagacccctg ccagccttgt
     3241 tgtcttaacc aaagataagc tagtttcttc agagaaccct gccagtttaa ttgcagatgg
     3301 ggaaatttct atttccgaac agattcgtgc tcagataaat gattatcttg gtgagaatgc
     3361 attgagtccg attgcctcca cagacaaccc ctcgttacat cctaatgctc cacaagttga
     3421 aggttttcgt tttatcgact ctaacaaaaa gttctttcaa attttagttg tcgactatac
     3481 agaaaatggc gagaaacatt ctcgcggcta catgtttaat cgccgggaaa ctattgacgg
     3541 tcaaataagt gaaggggtag ccatgatccc caaaactgtg gatggtaaat atatacttgt
     3601 caatcgctat cgtgaagtat ttgggcttat ttctcccgaa acatccagat gtttctcggc
     3661 taaagattat aaagaaaagt ttttgaatga atccggaata tcaaatgacc ccgataatat
     3721 cgttactaca aacataggtc agcttattga aactcatgac tttgaagctg gtgccaacga
     3781 gctggtcgta cttcaactta ctgaacctgt gactcataca tcagaacatg tacccaaaag
     3841 cttagatgga agcttggaat atgctaagtc tgtcgaatac tccgacgaag agatacatca
     3901 gttactagcc agtggccgaa ttagttgtga gttgactgct gccaccatta ctctagaaca
     3961 actgcacacc gggaagttaa tccttaatcc cgaattaagt aatgccgact taaatctacg
     4021 tcttgaagaa tattactccg ctgccgattc caagtattat cttcgtccac ctcgagcaaa
     4081 agaacacact ggagttagac aagggatgaa taagttccct aacagtgggt gtgctctaat
     4141 atgggggctt actgagtatt ctgataatca acaggatttg ccatctggta aaaaatatcg
     4201 cgacatccct gtttcggatg ttgtcgagtt aatcagagct ggggcacttg attcttctgc
     4261 tatttcgcat gtatgtcaga ttttacgcca ccaaggtata ctagttcttt cgccaccttc
     4321 tccccctctc tcaccgcaaa attagtcccc ctatcctccg gataaatcat cgcaaagttc
     4381 gcctgataca catttatttt gatttttgat ttttgaattt tgaattttcg tgtaacgatg
     4441 tgttgtgcat tcttaaattt ccatacccag gttttatctg ccccattaaa ttttggatat
     4501 attattttca aataatcaaa aaatactttt ttaatttctt tctcactttt atccatcaat
     4561 ccaccttcat ttggaatata tgtccccatg taataaacat gtttgccacc gtactctctt
     4621 ttgttaacta agttagtgtg ttgaataaag cagagaatag gggagtcttt atcgttaata
     4681 ttatgccaat aatatttact caagttctgt ttactagtaa atattaccgt aatcgccccc
     4741 aaatactcca cttcttttga cgctcccgta tataaaacct tatcaaattg ttttaagttc
     4801 tcagtggtaa cgtttgtgct taaaacaatt ttcccattaa ttttttttgc caaagcttct
     4861 gcaatttgat aaaaccctcc atgtagatat cctaattttg ccgaattccc tcgagtatga
     4921 atcctcgccc agagccaagc catacttatt tctttgtatt tatcatgaaa ctttcctctt
     4981 aaaagtggct cccatatcac tttatatgct ttctcaccac accacttttg catccattta
     5041 taggccaatt catcttcata cttgtgccaa ttattgtcta gttgtagcca nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tcctgatcgt agtagagtgc tgtcttttct
     5161 tcatgccatt ttaatttatt ttttagtccc agttcttcaa ttaggtcgat aatatatttg
     5221 tcagtcgaaa aaatatggtg ataagctttt tccagattag tcttgccaat ttgaaatcct
     5281 cccattaatc caccaatatc actagatttt tcaaatattg tcacttggtg tcctttttgt
     5341 gacaatctat atccggccac caacccagtc attcctgccc caacaattgc aattttcata
     5401 ttaataagta attgtatctg tatcagagat aaaggacaat atttatatat tatgaaaaag
     5461 aacacagaag aaacacaaat tgct
//
LOCUS       DDWP01000080           18113 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_25836, whole genome shotgun sequence.
ACCESSION   DDWP01000080 DDWP01000000
VERSION     DDWP01000080.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 18113)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18113)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18113
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_25836"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         5607 a         3623 c         3250 g         5633 t
ORIGIN      
        1 ttatcggtta actttgttac caaatcacgt ataccactag tgccaaatcc tactccagtt
       61 tccactctta gatcagttaa tttaattttc atcattagtt attttaactg tataatctga
      121 taatgacaca aatagatact caactcaaaa tcgaagatat aacaattggt actggcccag
      181 aagtcaagtc tggtgacaat atcttaattc actatcttgg aactctggaa aatggtcaaa
      241 agtttgacag ttcttatgac cgcaaagagc catttgaagt tcaaattggt gttggttatg
      301 ttattaaagg ttgggatcag ggtgttgttg gtatgaaagt cggtggtaag agaaaattaa
      361 ctatacctcc agaattaggt tatggttctc gtgagattcc tggagttatc cctgctaatt
      421 ctactttgat ttttgaagtc gaactcatcg aaatctttaa cgactaaaat cttgacactt
      481 cggcttttct tcgggtataa ttttttctta gtttaaagtt atttttactt tttacttttg
      541 aactttgttt ttacccgtat ggatttatac gaaaagatgt ctattcttgg tcctggtgct
      601 caatacgata cttgtggacc tcgtgacttt ggtcaaacca ccaatattcc tggtgtttac
      661 catgcccaat tcaatgggca ttcttgccgt ttgtttaaag ttcttcagac caatcagtgt
      721 cgcaacaatt gtcactattg cgcttttcgt cgtgatcgtg attgttccag gactactgcc
      781 agttccgatg aagttgcttc ggcttttgac tcagccttcc gtcgtcggtt agtcgatgga
      841 cttttcctat cttctggtat tgtcaacaat gccgacacta ccatgaccaa tctcctcgac
      901 actgttcata tccttcgtca gcgttatcgt tatcaaggtt acgtccacct caagataatg
      961 cctggcagct ctccttctat tattcgtgaa gccgttaaag ttagtcaacg aatttctcta
     1021 aatatcgagt ctcccacaga gtcagacctg agcagtcttt ctcccgacaa aaaccttaaa
     1081 gacggatttt ttcatactct ttttgagata aacaaacaaa tccgtcaagt taagttttat
     1141 ggtcttcgta cccctagtct gactacccaa ttcattgttg gtgctggtcg ggagaccgat
     1201 cgtgatttag tttctactac cagtcttctt tactcccaat ttggtctcaa aagagttttt
     1261 tattcttctt ttcgtccagt cgccgacact ccactagctt atttaccgcc atctccattg
     1321 attcgtcaaa accgtcttta tcaaaccgac tttctccttc gtttctacaa cttcactcca
     1381 tccgatcttt cttttgactc gctcggaaat cttttagact ctgttgatcc caaaacagct
     1441 tgggcaaatt ctcatccaga atattatcca gttaatctaa atcgtgccac cgtcgattct
     1501 cttttacgtg ttcccggtct tggcccgacc tctgttcaaa aaattatttc tctccgtcaa
     1561 cagcatccca tattttctct aacatctctt tcacatcttc gtcttcaagt ttctaagctt
     1621 atgccttatg tttgctttta gtataatcaa tcatgagcta tcagccgtca caaaaaatct
     1681 tagataacta tgccaaagta ttggtcaatt atgcacttaa tagtgggcag ggtattaaaa
     1741 agggtgaagt ggtttttctc caagtacccg aatcagccaa gccactatta ctttctcttt
     1801 atcaagcagt cttaacttct gggggccatc ctattgttca gtatttgccc gatgagatgg
     1861 aacatcaatt cttcaacctt gcctccgatc accaactaga tttttttcct gccaaattcc
     1921 ttaaaggtaa agtcgagcaa gccgaccact tcataactgt tctggccgag acaaatctgc
     1981 acgagcttga aggtgtcgat cctcaaaaga ttatgcagaa gaatattgct ctaaaaccat
     2041 atttagactg gagaaataaa aaagaaaatc tagggaaatt tacctggact cttggtcttt
     2101 atccgactcc agctatggcc cgagaagcca agactactct taagtcatgt tgggagcagg
     2161 ttattagagc ttgttatctt gatgtctctg atcctattga aacatggcaa tcaattggcc
     2221 atgaaattca tcgtcttagg gcttctctca attcccttga tattgactcg ctcaatatca
     2281 tatcgaccaa taccgatttg atgattggtc ttgataaaaa ccgtcgatgg ttagggggtg
     2341 atggtcgcaa tattccttca tttgagatct ttatttctcc tgattatcgt cggactcaag
     2401 gacacatctc tttcaatctt cctctttatc gatatggcaa tgaaattcgc gatatttatt
     2461 tagagtttaa taacggtctt gttactaaag ctaccgcttc aaaaggtgaa gccgttttaa
     2521 agaaaatgat tgctaccaag aatgccgata gagtgggtga gttttctctt acagaccgac
     2581 gtctttctcg gataaacaaa ttcatggccg agactcttta tgatgagaat tttagtggta
     2641 gatacggcaa tacccatctt gccctaggtg cggcattccc tgagtcatac ccaggtaaga
     2701 tcaataagat gtccacgtcc gactttgata aattaggctt caatgattct gttatccata
     2761 ccgatattat cgccactacc aatcgtactg tcaccgccaa tcttaccaat ggtaaaaaac
     2821 tagttatcta caaaaacggt aaatttaccg tttaactatt cttttgaatt attttccagt
     2881 gatataagta cagaggtgta ccgattataa tcattgccaa agagtttgat aattcgcttt
     2941 gtcgctgttg ttttacttgc tggtcctgtt gagccatttg ctcttccatg ctcatatatc
     3001 ctggctcacc ttttacaccc attgaataaa taggatatct atcagcatct tttaggataa
     3061 aagtttttaa tccgaggtta accatttgta ttcctccgat cactaccacc agtaaaccga
     3121 caaaagagaa tagatataaa tagagcagtc ttaaactaaa tttcataatt aatgctatca
     3181 tagatcatgg cattttcaat caacatcgca atcgttactg gtaacgttac ccgtgatcca
     3241 gaattaagat tcactccaag tggacaggct gtttgtagtt ttggtatcgc taccaatcgc
     3301 tcaattaaaa aaggtgatca atgggaagat gtcgccactt ttcacaatat cgttcaatgg
     3361 ggtaaagccg ccgaatatac tgctagtgtt cttaaaaaag ggatgaaggt atcagtttcc
     3421 ggtcgtattg acaatcgctc ttacgatgcc aaagatggta ccaaaaaata tattagtgag
     3481 attgtcgccg acacagttat tcctttcacc gatagacatt caaattctgg taccgagtat
     3541 cctaccaaaa ctcaagagtc caataatgta gaacaattac ccgacaagac cgttgaaaca
     3601 gtcgaagaca ttgttatacc tgatgagatg ccgttctagt tgtccctcta actattgccg
     3661 tcctacggca tccaggcatt acacaagtca cgttcgcttc taatccaacc ataccatcat
     3721 tagtatcagt atatccagtt actctaccat tacctttatg tccatcgtgt tttcccagta
     3781 ctgccggtaa tacttcaccc attgtaaatg gttctggtgt agcaaacttc ccctctccaa
     3841 caggacgttt aattactatt ctgtctgctg tctcaatcat atggcgatta tatcatccat
     3901 tctctccccc cccccttttt ttttgccacc aagcacctcc cactgtaatt cctaccaccc
     3961 aatcggttct ttcccgttaa aaatgagata agtattagtt tgggaaaacg gtttcgaacc
     4021 aaagaatccg ctatgagccg caaaagggga cggatgaggc gactttagga ctaagtgttt
     4081 tgtgtcatcg attactttac ccttgtcttg ggcatattta ccccatagta taaacactaa
     4141 gttgtctcgt ttttctgaca aaactttaat taccatgtca gtaaattcct cccaacctct
     4201 tttttgatgt gatccgggtg tgtttgctct caccgttaaa gttgagttta gtaaaagtac
     4261 tccttgtttt gcccaccgac taacatctcc gcttgttcct aagtcccttt ttatttcttt
     4321 gtaaatattt tgtaacgatg gtgggagtcg aacaccattg ttaaccgcaa aacacaatcc
     4381 atttgcctca ccgtcaccgt ggtaggggtc ttgtcccaaa attaccactt ttaccttatc
     4441 aaacggtgtt tcccaaaatg cccgaaatat aaatttggat ggagggtata cagtcgatgt
     4501 cttatattcc tctttcacaa attctgccaa tttcacaaaa taatcctttt caaactcctg
     4561 ttccagcacc tccttccacg acggttcaat taatactttc atactttaat ctatcatcaa
     4621 accattacct tcttaaagtc tagaaatagt gaaacaaact tcggcttatc ttcattctta
     4681 tgagaagatg ggttctcctc ttctctaata gcatcacatc tttgagagag taaggtttta
     4741 tcaatagaat attgaatttc ctttccgttt ttaatataaa ggtttttaat ttcatcacta
     4801 tcgtcaccaa ctgccacctt actaacagct aataattcaa gtgatttttt atccgtctca
     4861 tcacagatat cttttaaata cggtagatat attctatatg agtttataag agaaaaatca
     4921 acctttctgt ctttttgatc ttctctgaac gcagccacta tacccgatac ataatcaata
     4981 tctgaattga gtttttcgac agatgaataa agatcagcat acatatttat caaatcaaga
     5041 cttaaaagtt taaggcccaa accactgctc aagaataact tatcaaatct cagtacaaaa
     5101 cggacgttat catcatttgt attttcaata gcatcagtta tcgatgctag atttcttgac
     5161 atattcacac gaagaggagt cagctcctgt tctagttgaa caatggtatt tctgtgctca
     5221 aagtattttt caactctttt gtcattaact cttcgcttcg attcaaaatg gttcgtaaac
     5281 caaccagcaa aaaatccacc aaatagtcca ccccaaaaac ccgctgccat ttcggaaagt
     5341 gcaaaaaaac tactaatgtt acccaaatca aaaaaagggg acggtgccat tgttggagtt
     5401 ggtaattgtg ggccaattat catgtgacct ctggaagaat tgaacttcct ttttaaaacc
     5461 cttgaggtct ggagacttct gttctgccga tgaactaatg gcccgagtct acaaattaat
     5521 tttgtggacc atgcgagaat tgaactcgcc ttttaagttt gtgaccccac ggttggagtt
     5581 cgggaaccaa ataatatctg aactcatctt ctattttaag acattcgccg aagatatatc
     5641 aaagctaaca aatcctattc aactactccc catttagaga atataactac aatgtgtact
     5701 tacaatgtat gtgtttggaa gacaatagaa aaatagagat ataattcaga aatggataaa
     5761 aacgataata atgacgagca ggggcaaatg tacacccagt ttttgaagca atgttttact
     5821 ttatggatca atccagagat tgaaaaaaga aaaaaagaag gaaaaatttc ggacaaattt
     5881 attattgttc gagctcagat tctacttcct ttgggttcac cggccattgt tagattgaat
     5941 aatgaagtaa aagcggttgt taaagctaag atcaataaaa aaatagaaaa ggatcaattg
     6001 ctatatgaaa gcgatgttga gagtatagaa gagattagtc taacagatga agaaaaggac
     6061 tttggacacg ccaccttcat cctttttaaa ggtcattgga tcttaagttt tagctttatt
     6121 tatgacaaaa caaaatctca atctttcaac aaaattggcc aacaatacct tcaatccgcc
     6181 acttacgatc ttaaaaataa aaattatcgt ccatttattg aatcattaag tgtttccgcc
     6241 gaaaattttg caaaggctcg attgtactta cttccagatt tagaggttag aaaattgaaa
     6301 aaacatagtt ctctagctag gaaagtttgc ttctacgcaa aaacatccaa cattattagc
     6361 tctgacttca aagacagctt taactctctt ttaaaactac gggataaagc tcgatacaat
     6421 ccagactttt caattaaggt aagagaggca agagcacttc atgaggcagt ggataatttt
     6481 aagaaagaag tattaaattt attatcaaca gcatgattta tctaattggt attaaccatg
     6541 atattcagat agggcaagtt gacggaaaac gcagccaagc atttgaatcg ttattgggtg
     6601 acttaatctc agatttcaat ataaaatatg tcgtagaaga gtggagtgac gaagcaagta
     6661 aaatatggaa agtggaaagt tcaaaggtaa aaagggttat aaatgaagtt gaaaagaaat
     6721 tagaaattaa aataaaccat atatatgtcg atcctgatcg aagagaaaga aaagaaaata
     6781 acataaaaga ggaaaaagat ttagttgaac aacacggatg gaaagggatt atattgtcag
     6841 gtgagcaaaa atgggtaatt gaagaagaaa tgcgggaaga acatcgaaag agagagagta
     6901 tgtggttgaa aaaattagcc cttatgataa gtgatagtga catcatattt atctgtggta
     6961 ctaaacatat tagtgatttt aataaagttc gcggggatgg atttgatact cttctagcta
     7021 agtcaggata tgaagttgaa gttatagaca aacgttttga cgaatggttt gagacgtagg
     7081 tgatttggac attgacatac acgcagtaac tgcgtatcat agatgatatg gagtatattg
     7141 aactgggtaa tctaattaaa acagagcgtg aaaaacgtgg atggggtcaa gcagatctcg
     7201 cttcggcgtt aaaaagaggg caacaagcaa taagtcgctg ggagaacgga aaatcaagac
     7261 cgaaacggga cgatttgtta aagttggcgg atcttttttc tggagatgtc gaggaatggc
     7321 atttaatagc tggttaccaa attgagagac cagatagtgc tttggtacca tttctaccct
     7381 tggatagctt aacagcggaa aacttcgagc tattttgtcg tgatttaata cagaacctca
     7441 acccaggcgc tgacgttcag cgatatggct ctcaaggtga cgatcaagaa gggattgaca
     7501 tatatgctaa acataccgat ggtaaagttt tagattacca atgtaaacgt cataagcaat
     7561 ttgggccaac cgatgttagt aatgcgatct cagcgacaga ttttgtggca agccgccatc
     7621 acttactgtt atcccgtagg gcaacgtcaa gagctaaaaa aactatactt atgaataaaa
     7681 actggtccct ttgggactta gaaggtatat cttctaaaat acgctatctt gacagtgatc
     7741 gtgctgtaag gatcgttgaa acatattttc ccggctggcg taaaaaattt ttaggccttg
     7801 aggagcccag tccttggctt acaccacaag agttttatca attactagca aacaaaacaa
     7861 agttatttag tcatgggtgg agctttgtcg gtcgacagaa agagataaaa ttacttgaag
     7921 agtttgttga acaaaaagag ttacaagcaa ttgttgttag tggacgaggg ggtataggca
     7981 agagtcgaat gctgaagttt tgggcagatg aaatcagcaa agtagccgtt gtcagattcg
     8041 tgtcgtcggg aactgaagta aaaccaggta atctagagtt acttcccact ggaccatctt
     8101 ttttagtgat tgacgatgtt cacgaaagac ccgacttggt caccatttta aatggaattg
     8161 cacggttaag accagagatg aaactagtgt tgagtactcg gccatatggc ttggcacgtt
     8221 taaacgacga tttaactcgc gtaggagtta gttttgaccg tgatcgtcaa atcaatttaa
     8281 cagaccttag tgttgaagac tcgcgagttc tcgcagaaga gattttgtcg gatgtcggcg
     8341 gtgatataaa atttgccaaa cgcatcgcgg aaatcaccaa agactgtccg ttagctaccg
     8401 taattggaag tagattggtc ggaaaaggac aaattaaacc cgagctacta aataacgtcg
     8461 aggcttttag agaggaatta ctcctaagtt ttagaaatgt cattgccggg gaaattggag
     8521 gatcagattc tgaaaatata cgtgacttac tcgactattt atcactagtc caaccgttta
     8581 atccttctga tccaacattt ataaagtcga ctgaagagac tttaaaaaca ccgtttgaca
     8641 aaacaatgag agatattgga aatctggagg acgctggtgt attacttcgg cgaggtaata
     8701 aattgaggat agtaccagat ctactcgctg attatattag gtcaaaggct tcatataatg
     8761 aaaaaaacaa tatgtctact gggtttgcag agagggtatt caaggtggca gaaaacgatt
     8821 tagctactaa tttgctagtt aacattagcc agttagattg gcgtttatca atagacggca
     8881 taagatcatc tttactcaac gaggtgtgga gtagtcttaa agaacaattt agagccgcaa
     8941 aaatctttga aagatctttg atattgaaag caattgaaaa ggtggcctat tatcagccag
     9001 ggcacgctct ggactttatc cacttagcgc tagatgaacc aaccaatgag atagaagaag
     9061 aacagcctgc ttttttgttt acagaaccaa gttacgatat ggttataaaa gaggttgcac
     9121 ccatacttcg atatgttgcc tacaaccaag atcatattat agaatcgttg gaactactta
     9181 aagatcttgc aaaacgagac catagaccaa caaattcaca cccggatcat ccacttcgaa
     9241 tactacaaga attggcatcc atcgaaccag gcaaacccat agcttatata gatgctgtgg
     9301 caaatcatgt gattacctgg ttagcatccg aaacaacatc agacttttct ccttttgatg
     9361 tactcgacga actactcaaa actgagggcc accagtctga atcgaaagga ctaacattaa
     9421 cgctaaaacc ttttagagtt aaaccagaag ctgtggccga acttaggcaa cgagtgctta
     9481 acgttgcatt cgaagcacta cataatagtt cgatagaaat atcctcacgc gcgataaaaa
     9541 cgatagcggc gggactacat tatccatttg gcttttcaat taaaaacata tctcaagacg
     9601 aattggcagt gtgggacaag gagtttctcg tgacactaag ccgtcttaaa gacgtagtta
     9661 gggatccgaa gattgatcca tttatttcta tagaggtaag aagtgccgta tcgtggcact
     9721 ccaattatag taattcggga acaaaagaag tcgcacaaaa gatccttttg tccatcccga
     9781 aaacaattcc acatgaagta acaagggctc tggttgatag ctgggggtgg tcattcgaaa
     9841 gaaaagacgg gaaggtgggg cgtgatgaag ccgagctaat aagatggcga gacgacttgg
     9901 ccaaggaggt aacaactcaa tacaaagata acttcgacgg acttataaaa atgctggaag
     9961 aacggataga ctcaatgaat atcgcgaaaa tatctcgaag tccagacgcc ggtccatttc
    10021 ttggatcact tatggacagt tctattgatt ttgctgagta tctcggtaat tatttgttgt
    10081 ccaactcaaa gtcaccgtta gccatttggt tcaatgttgt aataatggta atgaataaga
    10141 gtaaacctaa cattgcgaca tcttttattc aacgagcggt taatgaaaat gtggatgtcc
    10201 ttattcggtg tgttgcgggt gctctggggt ggggtttacg tggtgtcaca ataactaacg
    10261 aagaaataga aattattaag attctcgctg tctcaaaaga cccttggata aggcaatcaa
    10321 tcgttaatgc tgttaaaaga ttctctccgg aaaacaaatc tatcgcccta gaaattttat
    10381 taagcatcga cttaacagat tctcaaaaag ttgctgaaga agtgactggt gaattcgaaa
    10441 gtgagtatag tacgtttaaa atcgcggatc tttctgaaga tcaactacag acgttattaa
    10501 accagcttgt caaatgccca tcaattgatg gttatgaaat atgcttattt ttaagtcatg
    10561 tttctttatc aaatcccgag ttaacactta aaacgttgca aggtcgtgtc gaatacaagg
    10621 aaagtaatcc aaaactgaag gagtatgagc cactgccaca ttctatggaa aataacatac
    10681 aactacgttt tcaagagaca gcaaattacg aggaagtttt gagaaatgta cgggactgga
    10741 gcacagtaga taccggaaat tggatacgtc catattttgg actaatactc tttaaactgg
    10801 tttcctcaag ttacgatgat gtcaccttaa aagtgcttgg agagtgggta gattcaactg
    10861 acgagaaaca gttggaagca gttgcagatc tcttaactga ggcaaacaca tcttttattt
    10921 gggaaaacca acaatatata atcaatatct tagaacaggc ccaaaaacat ggcatcgcct
    10981 gctataaaag agtttgttcg tccatgcaca ctttggctgt acatggaggg cgtagtggaa
    11041 ctgttggtga accgtttccc gaagacataa tccagaggga tcgatcatat gaacttatga
    11101 cgaagctgcc cccagggtct cctgcacaac gttttttcaa attgttatac gaagaggcac
    11161 ttagtgaaat taaacgtcat actaaagagg attttgattt tgaataatac aatgtcgact
    11221 atttatcaaa ccccaaaatt tgaaaagtgg ctttccctat atgcagacaa tagcaatgca
    11281 attacattca gcaatgccac tcagtcagca ctcattgctt acaatacaac taaccaaaat
    11341 atggcggcgg tgatcgggag taaaaatata agaaagtata agatgttagc ttcaatggtt
    11401 gcagaaaaag agggttttga tttcgcaaca ctgattaagg ttggaatcaa aaaagtacta
    11461 gaagggaatt acaatgactg ggaaaagttc atgaaacaaa tcggttactt tgactaactt
    11521 tatattccac actgctcttt atacgcagac tgagaaataa cccaagactt tatacttttt
    11581 actaaaccgt caaggaagca ctgatcttta tccttcgcgt tcgtacagcc tttggtggcg
    11641 tcgtagcccc agttgacaag ttgctcgaaa aatctaactt ggttgcttga ggtactacct
    11701 gtttcaattc tagaagttaa tgaattctgc tcttccttgg taagagaaat catggaaaac
    11761 aatgtttgtt gagcgtatgc ttctgtttct ttttgaacac aatctaattt aggaccgcca
    11821 aagtactctc ggacaaattg tctggcatgg gttaactcat gtgtgagaag taaggcagta
    11881 gataattcat caaggttgat ataactttgg tcaacttgga ttactaagcg ctcaggtgtt
    11941 gagaccgact cgtcaaaaga aaacatacct tctgccccct ctgaatttaa attggtgaaa
    12001 cttatatcaa tgcagttttt catctttaaa taatccattc caacattttg ataccagtct
    12061 ttttgatttt ttgaatatct ttcaatgata aaagaaactg ctctttttaa ttgctcaggt
    12121 attgggtatg gttccactct cgcacaatac tcatttttag tttgtggtac tgtcgtactc
    12181 acagttgatc ctttaaacat tgttgcaaaa tctaagacta ccgataagct acaaaaaact
    12241 acaaatatgc cgattagata tttatatttt cttttcattt ttgtatttgg acccactctg
    12301 gtaatgtgat cggctttgtt tgaggctaaa taaccatctg taaacgaaaa taggtatgac
    12361 aatagcagta cctaatatca gagaaaagat ataaagtgac caaagaatta ttccgtgagg
    12421 atgaacaata gtactttcat caatatgtag gacatagacc gagatcacac cataaatgtt
    12481 gactagtgta aacctcgtaa caaaccacca agcgccaatg gacataaata tcaaagcaat
    12541 cctataaatg gttttcattt taatttaaca gaatgttttt taagcacaac accgtgccta
    12601 aatatgaact atatttcctg ttcgaattat gacatatatt ccataatttg ccaactatgg
    12661 caactatata cgagaagttt gggcttaata taaagaaata cagaaaaata actggtatga
    12721 cccaggagaa actggctgag gcagcaaagg tagatccaaa atcgattatt tctattgaga
    12781 atgggactag gaacccgaca ctaaggacaa tatacaggct ggccaaagca tttaagacaa
    12841 gcatcgagga gttggtaaaa ttctgaatct tttagcatca ttcacggctt ttttatatca
    12901 atatacttac ttgatttttt cacaggtatt ggagtagtat tagatactcg caagggagca
    12961 catagtacta gtacacaacg caaattttgc ctttaacggc aacgaataat tatctttcga
    13021 gcatattcgt tggccgcttt tttttggcct aaagacaaaa tatgcttact gtgctcggag
    13081 caagaaaagt acttggaaaa caatctgaca caatgtctga tgaagaagtt ttaaaagaca
    13141 ttgaaaccgc tgaattattt aaggagtttt tcatcggact taccattgaa aagaaagcgt
    13201 gccataattg cagtaatgga aaaaccacga gcagttatat atctaagagt ctctgaccca
    13261 agtcaagttg aaaataatag tcttaagacc caaagtgata cttgcaagca atatattttg
    13321 tcaaaaggtt acgaattagc aagagagccg tttagggatg aaggtttctc tgcaaagcat
    13381 acttcaacaa gacctgcact ccaggaacta tttcaatact gtactaataa aaagaatcag
    13441 attagctatg ttattgttta tcgaatagat aggttttctc ggaataccca agagggacta
    13501 gttgcagaag cgtttttaag taaatatggg gtggaattac agtcggcaac tgaaggtatt
    13561 tcaagagatc caacgggtac aatgcttaaa acaatctttt tctcgatcgc ccaatgggat
    13621 aatgaaatga agggtttaag agtaggagat aatcaaaaaa cgatgtttaa tgacgggtac
    13681 tggtgttgga aaccaccaat tggttatcga cgtccttacg gtatgaagag tgatagaaag
    13741 ggtaatgcct gtatcattga cgataacctt ggaccactgg taaagatttt attcgaagag
    13801 ggagcaaaag gtatacagac aaagcaagca ttggcaaatt atattaacaa gttaggattt
    13861 gaaaaatatc atggcaaaaa ggcagatgtt aatcttgtat caaaaatagt atccaaaact
    13921 ttttactacg gcttaatgta tgcgcccaaa tggaaaaaat atcaattagg attacatcaa
    13981 ccattgatta gtgaagatat ttggcaaaag accaacaaaa atctatttgg cagaaaggcc
    14041 aacattatga acgatataaa attattccct cttaaaggtc tgatttattg ctcaacctgc
    14101 gggggacgac ttacaacaag taaagctaga ggaaacggcg gaagttatct ttactatgaa
    14161 tgtaaaagta agaaatgtgc gaaaaatcaa agaataatgg ctagagacgc tcataaaaca
    14221 tttctagata ttttgaaaac tttaaaacca aaaccaacag ttttaaaact gtttgaacat
    14281 ctcgtattta gtgattggga taaactaatc gaaggctgta aaaaacaaat tgcaatgatc
    14341 gataatagaa tagatcaatt aaacaatgac ttaacagggg taactaaaag caacgataag
    14401 ggaatcttga ctgacgacga agctaaagag agagctaata aaattcgatc agaggttgtt
    14461 gcattaaaaa taagtaagtc agatgttgta attgaacaat acgacacgga gattgtacga
    14521 ggttttacgg aaaattttct gaacaattta gataacctat ggaaccatat agatttaccc
    14581 aaaaaacagg cattacagaa caaaatcttt ccaaacgggc ttatctgtga aaaccatact
    14641 attcgaacca acggtttatc ccaatcattc caatatatag aagagttaaa tactcaaaat
    14701 tctcctttgg tgactccacg gggaatcgga ccccgattgc caggatgaga acctgacgtc
    14761 ctaaccgtta gacgatggag cctttgaatg ttgtacaata aagacagatg aattttaaca
    14821 aatatcgatc catttttctt ctttcaatca taactgtctt ggtttctcta tttttctggt
    14881 ttggctttta tcttaacgtc ccttcccgtc ttggttttcc cgataccacc cttgaaactg
    14941 tctttgccaa ttatgatggt ccaaattata tggttattgc caaatgtggc tatgataaat
    15001 cctgtatcgg tagcaatttt tctttgcctc aaccactaga atattatcct gcccatcttc
    15061 ctggcttccc tctattaata aaatacttta gcttctttac ctccactccc aaggccatgc
    15121 ttttgactag tctttttggt tcaattttct tttccttaac cagtttttat ttttataaac
    15181 tttttcttaa agaaaaatct tccctctact tagccatatt taccatattt ttcccggctc
    15241 gtttattagc cttacgatta gtcggtgctc cagaaacttg gtttattggt gccatattat
    15301 tatcaataat tttatttaag aaaaacagat ttttcctctc cgctctctta ggtgcttttg
    15361 cacttctgct taaaagccct ggcattctac tttttggagt ctatggtctt ctctttttga
    15421 agaatcgaaa tttacgttat acctggtatc tattaatacc agctactgct ttaggaattt
    15481 tctatgctta ctttcttcaa acaggagatt tttgggctta ctttaatagc ggtgacaacc
    15541 gtcatctcaa cccgttacct tacctcgttt ttgtcagtaa tagtacctgg atcaacacca
    15601 tttggcttga agatctcgtc tatatttacc ttatcgctat ctatggcctt caccgtcttt
    15661 tcaagaaata taaatacgac attatagttt tatttccact tgtctacacc ctggccgcaa
    15721 tttttgtagc tcatcgcgat atcagtcgct acatcgctcc agcttatcca ttctttattt
    15781 tggctttttc aaaattttta ctcaagaaga atgtcaaaat aattttcctt ttacttttac
    15841 cagctattat tctgtacaca attaacttca taattggtaa cactgccccc atcgctgact
    15901 ggacaccgta tctttaaagt tgttttagga aaatctttcc catttcttca ttaactagat
    15961 tgttcatcga cattttggtt attgtcgtcg atatttcaat cacactttta ccaagttctt
    16021 tagcaatttc gtcaacagtt aacggctcac attccaacaa cttatagatc atattttctt
    16081 cattattcaa actgacttca aataactttt cctgtgttat agcaccattt ataatatctc
    16141 ctacctcagt caccatttta gcctgattgt tttttataag ccagtttgtc ccctcacttg
    16201 tggcagaatt aatctgtcca gggatcgcaa aaactttctt actcatttcg tttcctagcc
    16261 tagcagttat taggcttcct gatttcatcc ctgcctctac caccaatatt ccctctgttg
    16321 ccattctagc cactactcgg tttctcattg gaaacgacca cactgtcgct ttaaactctg
    16381 gctcaaattc tgatattact aaccctccag aatttaatat atcggcatac aaaccatcat
    16441 tatcgacagt cgttaaataa tttaatccac taccgagtac tgcaattgtt ttcccaccca
    16501 tttcgataca agatcgatgt gcttccgaat caattccata cataaaacca gatattatag
    16561 ttagcttatt agctactaaa tccggcataa attttgccac catctctcgt ccataacgag
    16621 tcatctttcg actccccacc accactatag ttttctcaaa caatttttca tcccactcgc
    16681 cacgataata caatttatct tttttctgta tcggccattt cttccactca tgcatataaa
    16741 ttatcgtaat atctaatttc tgatccttca atgcacaccg catctattct gtatttctca
    16801 aacacctttc tcatttccgg atttttcatt aagtaaattt gcgccactcg ttttacttgt
    16861 gccaattttc ttttattaat catttcttct ggtatcccca ttcgatcatc acttttatat
    16921 tttacttcca caaataccag ccaatcatta tcactcatta ttaagtcaat ctctcctttc
    16981 attcctttat caattgaaaa attcgcttca ataaaattga accccttttt gatcaaaaat
    17041 tcctttgcct cttcttcccc gcctctccct tttttgtaat taaacctctt ttcactcatt
    17101 tctcactcta aattgtagtg cttctgccac atgcttgtct gtaatttcac tcgaattatc
    17161 aaggtcagca atcgtcctac tcacttttat caatctaaaa tacgttctag ccgataaact
    17221 aaacttgtta actgcctgct ttaagaacaa atttgcctcc ggtgtcaact tagcaaaatc
    17281 ttttaaatga ctgtttctca tatcagaatt acaatggact ccaactattt tttcaaatct
    17341 tttcctctgt atttctctgg ctgctacaac ccttttttta actacttcag acttttctcc
    17401 gccaccatct tttttactat ccagcattaa tttatttaca tccaccgccg ggacatttaa
    17461 atgcaaatca attctatcca ttatcggacc cgacaatttc ttttgataat tcataatctg
    17521 atggacgtta catcgacatt cttttttaat gtcccccaaa aatccacacg ggcaaggatt
    17581 ggccgctgcc accaaaataa attgtgctgg aaaatttatt gacccagcgg ctctagatat
    17641 agtcacattt ccatcttcta atggttggcg caatgcttct aatgaatttc ttccaaactc
    17701 aggaaactca tccagaaaaa gtactcctcg atgtgccatc gacacttctc cggggtgggg
    17761 gattgaccca ccaccaatta accccaccat tgaagttgtg tggtgtggtg aacgaaaagg
    17821 cctttgcctt accatcgatc ctcccgctgg aatattacct gtaattgaaa atattttggt
    17881 aacttccagg ctttctctct cagtcaggtc gggcaatatt cctggtatcg ctcttgataa
    17941 cattgttttt ccacttcccg gtgggcccat tagaaaaata ttatgtccac cagcggcggc
    18001 aatttctagt gctcttttgg ccatctcttg tcctaatact tcagcaaaat caaattctgg
    18061 atgaatgtca cctaacacct cttcggtttt gattaatgcc aaaggttcaa tta
//
LOCUS       DDWP01000081            3530 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_24448, whole genome shotgun sequence.
ACCESSION   DDWP01000081 DDWP01000000
VERSION     DDWP01000081.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_24448"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1205 a          488 c          801 g         1036 t
ORIGIN      
        1 ctactgtctt tgatgcttga aaaaatagag caggaaaatt ggcgacaagt tataaaatac
       61 tatttcccct ggatcggatt cataatagcg gtaggagcaa tgtcttttat ttacaaagag
      121 tatgatgtgg tgaatgtaaa aactgctctg agaaacctag ttgtgccaat ggcaatatta
      181 ggaatatcaa tgatagccac attattgttt agaaaaaagt cattatttgt accactgatg
      241 attgctctac taatggtgga tatgtttaga tttggttgga agtatttacc attctcagac
      301 ccaagatacg tgttccccca aaccgaggta acagactttt tggaaaaaca gacaggggtg
      361 tttcgagtag ataaagaata tggcccagta ttgtcgccaa acacatggac aatgtatgga
      421 ctgatgagtc cgtccggata tgacccgatg gcagtggcta attacactaa aaagttcaac
      481 caagatataa ataatgacaa agaaaattat agccgttatg cagaggcgaa aagatacgat
      541 gccaagacat taggaaaata taatgtgcga tattttctag cattaaaacg atatgaggac
      601 tggcgaattg gaggggaaat gataaatgat caaatacaaa ttaccaattg ggagagagtg
      661 gccgaatcaa aaacggtaac agtgctggaa aataaagatt acttacctag agcctttgtt
      721 aaaaacaata ctgatagcgg aatggtagaa attacagaat atcaggctaa taaagtggta
      781 ataaattatg atttaaaaaa gaaaggtgaa gtggtattgc tagatactta ttacccagga
      841 tgggtggcaa aaattaatgg gaaagttgta tcaattgata aatatgaaga agtgtttagg
      901 agcgtagcag tagaagctgg gcaagggata attgaattta attatgaacc ggcgacattt
      961 tattggggca taaggataag catagtagca actctagtat ggctaggagt aatgttatat
     1021 tacttgcaga gatgtttaaa aaaggtaact ttataaaatt tatatttgtc tacctggtag
     1081 tgttttacag tctaactgga gtaatgtttt tgggattaaa gtcacatttc aaacaaaact
     1141 ttagtgaagg aaaactagag aaaatgataa gcgcaaaatc agcttggaga gaaatgacga
     1201 ctgatctttg taattttcaa atttatggtg atccggtgga tgcaggcaag gcttatgtca
     1261 aagttaattt tgaatgtgct gatggacggt cttcaaaaaa tactttatcg ttaattgcca
     1321 taaaagataa aacatttgaa gggataatga ctgagtttgc cagaataatt ggatttgacc
     1381 caaagatagt aacggataaa ggtagcgtgt ggaaatgtag tattgacaat caagaggtgg
     1441 tcgacagaaa aatgttaatt aatgagcaag caacaattaa ctgcaaatga ataaatttaa
     1501 aataattatc cagacaatag tatttatagg ggttctgata ttgagttata aattaagact
     1561 ttttaactac gacaagattc ccctgccagg ccagtcaatg gatgaatatt catattcgtg
     1621 ggtagggttg tcgctgatcg aactaggggt accggtgggg atatcgggga ttgacggata
     1681 caagaatagc gatatgcgat atataaatgt ggatagagtg tttcaagaaa ctgctaaagg
     1741 aaatccattg tcggtggaaa gaccatggtt tgatcaccct ccattaatgg gtgtagtaac
     1801 gggagggttt gcgtatatta aaggagcgag ggtgtttgaa gatactatat cggcatttat
     1861 tagaaaaccg atggtgtacc tagggacaat aagtgtggcc ttggtgggat gctttgtaat
     1921 aattaatttt ggatttgtaa cagggatagt agcgatgctg atttatggaa caatgccgat
     1981 tgtggtaatc ggtagtcgaa tgatccaggc agagaatgga ttaataccga tgacgctgtt
     2041 gacgatgata tttttgtcat catacataaa atataaaaag ttaagatggt tgtggctcgc
     2101 gggaatagca gcaggcatag ggactttatt taaactatca ggggtgacaa atatattgat
     2161 ggtagggata gtactactgg taaacaaaaa taataataaa tggcaggaaa tagctatatt
     2221 tggaacagtg gctttatcga taactagcct attttttgta tacgggatgg tctatgattg
     2281 ggagacattc gtgagtattt ttaattctaa ctctaatagg ttttacggaa tcgggccggt
     2341 ggcgatatat gatctgatga ctcaagttaa gttgacgcat aacaaatatt tgactgattt
     2401 ctggccactg gttggctggg ttggctactg ggtattgatg gcaaaaaaac aaaaaaatgt
     2461 gggagagata attttaatgg tggcaacgat gtcgtactta gtgacttata tatttatggg
     2521 gagtcagtcg tacggctggt atgttttacc tttctggcca cttttggcaa tgtcactggc
     2581 ggtaactttc gtagaattat ggaaaaatgg acaaatgctg gtagtttggc tgtggttact
     2641 agtgccaatt ggaggcacaa taggtaaatt gatggatatt aataagtttc aagaatatgc
     2701 aggaatatgg agatacgggc tggcattgat aactgtagta atgctgataa gtcagctaaa
     2761 aaaagatcag aaatacctga ccatgttttc taaagttgtg gtgatagtaa cttttctaat
     2821 agcaatatat ttgaatacaa aatacttggg aatgtttgat atcaactatt ggtataagat
     2881 gtaaaattct ctttaactac tttgtcgtat tcggcttggt cgttagtttt gaccaatgga
     2941 gaaattgatt ttagaaagct aggcttagtt gagaacaagg aaatcatccc ctggtcactt
     3001 tcgttagctg ttctcatact aaaatagagg tctaactttt cttgggccat atatttttca
     3061 aaatcatttt tcttgcggat aaagccaaat tctttggagc ttaccaaaag atcaagttta
     3121 actcgacttg attcaagtaa ttcaaaggcc aaatacggag tagcttcata caacccggca
     3181 cgtttagcaa cagatgcttg aaaaccaaac ttgtctactt caccccagtc aaaactagtt
     3241 agaatacttc cgtttttata aaaggtcaca ccagcctcag aaatttcaat acgattgttc
     3301 ttgctattca aaaaagcgat taaagcaaat acacttggta accaaaaaac aaataaggtt
     3361 actaccccta ataatccaat aatggggaac aaaaaataag ctgcgatagg taaggctatg
     3421 atttctccaa caaagagtgc cggaaaagga agttgatagg agaggagcgt gtatttcttc
     3481 ataaataata atctcttatc tataaagaaa aaacaatgtt ttggggggta
//
LOCUS       DDWP01000082            7967 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_15155, whole genome shotgun sequence.
ACCESSION   DDWP01000082 DDWP01000000
VERSION     DDWP01000082.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_15155"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2716 a         1226 c         1839 g         2186 t
ORIGIN      
        1 aaatattgat aaaaagtatc gaagacaagt ctaaggttcc gctagtaatg gatatggtgc
       61 tagccaactt ggcgccattt gaaagaagcc ggttgtattc aataaaagag caaaaaagct
      121 tggcgaataa cgttaacaat gttaacccag gcagtgatta tttttctcaa ttgggggtag
      181 ataaaaatgc aacagatgaa gaggtagaaa aagtctacgc taaggtgaca aaaacaccag
      241 aaattgagca agcatataag gttctaaaag acgaagattc tagaaaaatg tatcaagtat
      301 ctggggtgga gccaactatt gattatcgac taattggaga gaatgttttt tatatgcatt
      361 tgacaaagtt ttcaccgacg agcttggagg agtttgccag agtgacagaa aaagttgacg
      421 acagaggacc ggaattaaat agcttaatac ttgatttaag ggggaatatt ggtggagcaa
      481 ttgacggact cccctatttt ctggggccat ttattgggat tgatagttat gcttatcagt
      541 tttaccagca gggagaaaag aaagatttta agacattaat tggttggatg aactcactag
      601 tgcgctacaa aaaagtggtg attttaattg atgaaaactc ccaatcaacg gctgaagtga
      661 tggcggcaac tttgaagaaa tataatgtgg gagtggtgat ggggacgacg acgaaaggct
      721 ggggaacggt ggaaagggtg tttgaattga aaaatcaaat atctgataca gaaaaatatt
      781 cgttattttt agtacatcac ataactttga gagaagatgg gcaaccgata gaagggcgag
      841 gagtcgaccc gatgatttcc atcaaagata ctaattggaa aaaagaattg ttggccaggt
      901 ttggaagcag tgaattggtg aaggcagtgg agagtgtgta taagggtata taattgcgaa
      961 tggacgaatt attggattta gtgaatgaga atgatgaggt agtgggagag gtgtggagaa
     1021 gtgctacggt tggtcatcct gaattaattt ttagagaagt aggcatattg atatatgatg
     1081 ataaaaaacg attattattg caaaaaagaa aacttaataa aaaatcatat ccaggatatt
     1141 ggataattag tgccggtgga catatcggca aagggaagaa ccctgaagac gaagctcata
     1201 gagaactaaa agaagaactg ggctttgata cagttcttga atttgcattt aaactgctac
     1261 taacctaccc gggaaataga tcgtatgctt atggatattt tggtaaatac aatggaggaa
     1321 atattgtcgt ccaagaagag gaagtggaag aggtgagatt tttttcaaaa gaagaatttg
     1381 aagagttgat aaaaaatgat aaagtcgaac caacttcggc tgattggtgt cgagagtttt
     1441 gggaaaatag taaataaaaa aaatcccccg tttccggggg attttgattg caaagattgc
     1501 cacggcctac ggcctcgcaa tgacgtatgt gttacatcat tccatccata ccgcccatgc
     1561 ctcccatgcc accagatggc attgcggggg cttttttctc aggaatatca gtgatcaaaa
     1621 catcagtagt taagatcata gtacccacag aaacagcgtt ggttaaggct gatttagtaa
     1681 ctttagcagg gtcaagaata cccgccttag tcatggaaac taactcccct gtcgaggcgt
     1741 tataaccaat gtctgatttt ggatcagcga ggatagcatc tttaacttta ttaaagacag
     1801 caccaccatc ttcaccagag ttttgagcta acatgcggat aggttgttcg agagcgtgac
     1861 ggagaatagc aataccaact ttttcttcgt cattgtcggt aacaacttta tctaaagcaa
     1921 aagaagctct aagaagagcg acaccgccac caggaagaat accttcttca atagcagctt
     1981 gagtagcttc gatagcatct ttaactctct cgagacgttc tttcatctcg acttcagtag
     2041 ctccaccgac ttgaagaaca atagctccac cggccaattt ggcgattcgc tcggccattt
     2101 tttctttgtc gtaatcggaa gtacttttgg tcatttgatt acgcaattga gtgacacgat
     2161 ctttgatctc ttgttcggaa cctttaccac caataattct agttttgtct ttgtcagaag
     2221 taactgaatc ggcctgacca caatattcga gtggctcaac agtgtcaaac ttggcaccag
     2281 tttcttcgga aatgacttta ccaccagtaa ggacggcaat gtcctcaagc atagctttac
     2341 gacgatcacc aaaacccggg gctttaacag ctaataccga gaaagtacca cgaagtttat
     2401 tgacaacaag agtagccaaa gcttcaccgt cgatatcttc agcaatgatg acaaagttct
     2461 tggtgatttt gactaacttt tccaagaaag gaagaatgtc ttggatggaa ctaatttttt
     2521 tatcggtaat aataatgtaa gggcgttcaa tgacggcctc catggcttca gtgttggtag
     2581 caaaataggc agaaaggaaa ccattgtcaa actccatacc ggaagtttct ttggtttcga
     2641 ggcccatacc tttaccttct tcagcagtga ttaagccatc gcggccaact ttgaccatgc
     2701 tttcggcaat ttttttacca atttcgacat taccagcaga gatagtggcg acttgttcga
     2761 tactagcttg atcgtcaatt ttgatttctt ttttcatgga atcaagttgt tcgataactg
     2821 cagccagacc tttctcaagt ccataacgga tcatcattgg attggcacca gcggcaacat
     2881 tttggagacc tttgttggca atagcttgag ccaaaagtgt ggcagtagta gtaccatctc
     2941 cagcgatatc gttggttttg ctggcagctt ctttaagaag ttgagcaccc atgttttcat
     3001 aggcgtcttc aagttcgatt tctttagcga cagtgacacc gtcatgaatg acggtaggac
     3061 cgccccattt tttatcgata gccacgttac gaccacgagg accgagagta gtgataacgg
     3121 ctgaagcgag aatgttaata ccgtttagaa gtgcttggcg ggcgtcttgc ccgaatttga
     3181 gttgttttgc catagattag ttcgctagag cgttagcacg ctagagcttt agttaaaaaa
     3241 taataattaa ttgatgacag ccatgatgtc gtcgaatttg aggattaata aatcattgtc
     3301 accatcttta tattctttac caccccattc acgatagatg acgacatcgt cgactttaac
     3361 tggggcaaca acttcgcgtc catcttgaaa agtaggaccg ccaatagcga gaactttacc
     3421 ctgttgaggt ttatcagtac tgctttcggg caaaactatg cctgaggcag tggttttatc
     3481 ttttttttgg ggttggacta ggacatagcc cggaactgga tttatcatac gaaattgaaa
     3541 ctaataatta ataattatga attagggtcg aaatctattg tagcagataa tataccagac
     3601 tgctaatagt tttttcaagg gagaaatttg tagccgcggc cacggacagt aactaaggat
     3661 tctgacttga gaccgacttg ggataattta ggacggagac gttgaatcat tttgttaata
     3721 gcccagtaag aatgtgattc ttcggaactc cagatagtat cagcaagatc atcgttggtg
     3781 actaagtttc cagaattatc gagaagttta ttaataattt ttaattcttt ggtaccaaaa
     3841 atctgattgt gagagttgac aatatcttga caatcagcag aaaaataaat acctaatgat
     3901 cggcaataat ctaagctttg ttggtgatag ctggctgtca gacgatttaa attctggtta
     3961 cttaagttgt ggtgaggaaa gatttttttt aactcgaatc tagacataac aaaatcaaca
     4021 atggcagtac gttggctaaa agaaatagat ccgtcagctt gttgaggcca gaggaaacta
     4081 gagacaatgg cagtagccag atcgtcgaga ttggcatcaa ctcgaagatg aataatttgt
     4141 ttttggaatt tttgagaaga caagaggccg aaagaaaaac gggggccgta gttggtgacc
     4201 ctaacctcaa tttctcccat ccacttaatt tctctaggaa aaagttgact aatagcaggc
     4261 caaaacttat cagataaagt agcccagttc tctgaaagtt tatcgataat ttgtgtgtca
     4321 acagggacta tctttagttt tgaaacttca cgtttcataa gatcagttag ttggataggt
     4381 aaaaccgggc cgacacgagc agcatcgtgc caatagctag gggttagatg tttgtagaac
     4441 tctggagcaa cgacacaagg gatattcgga agaatctttg gactaataac aaaaccttta
     4501 cggttgtaga agccagaggc aatgatagca gtctgataaa gaagacgttc tttttcggtg
     4561 tcagaagtag aaatgaagct aatttttggg acatatgtcc tcataatgtc cagatattaa
     4621 cacaattaag cttataatac aaaatatgaa tcaagaaaaa gataaaaacg cattagaatt
     4681 agaaaaagtg cctgaaatac aaccagaacc tgaaaaaatt gttgataagc aggtagatga
     4741 taataaaact agtaaagtat tgttacccgt aaggttggtt agtggttgtg atggtgactg
     4801 tgcagatccc ggcataggag gaacgtgtat ggtagagcta acaccgagaa agtcagatat
     4861 cttacaggta tctattagag ttgtttaaac accataatgt caccaaggta caaggaagaa
     4921 tccactggta gctttatggt gggatctggg agagatatag atgaaatgag atttcaagaa
     4981 attgtaataa agacagcgga ctttgtcaca gccaggccaa gaaatatgga aaagtgcggg
     5041 gcattttctc atattgaagg tactgagtta gaattacttg atcatgattt tcagtttaca
     5101 attgacagaa gtaatgatcc taattgcgag ctttatttgc aaacgacaga tagatgtaat
     5161 cttaaatgcc ctggctgtgc ggtggggctg gataaaacga gtagaaaagg agctgtgttg
     5221 acaagtttat ctgaaatcca gcgaacaatc ggtgagacta tagctgtttt tgcagatgcc
     5281 ggcaaaaaac atttgaaagt taaatatgcc ggaggagagc caactctaca agtagagaat
     5341 ataaatgaac tgtcggaatt taccaaaagt gaagcagacg cacatgatct gtcaactgac
     5401 catgtgctga ttactaatgg cacattgcta actaaagata cagtaagaaa aataggtgat
     5461 atgaatatga gaataaatat tagcctttgg ggcatgggtg atacaaatga tagagagagg
     5521 ggaaacatgg gcaaaccatc atttaaaaaa atctttaagg gaattaatta ttgtaaggaa
     5581 ttaggtgttc cactaagtct aacttatatt gttaccccaa ctaatgcagg agaactaaca
     5641 ccatttataa atacttttgc aacagacgct aatacaaaaa tttcaattgc tttatttcga
     5701 cctcagaagc cagaacagtt gaagcaaatt gagactagta tagaaaaaat gatagatggc
     5761 atacgagacg gaataagagc cgcggaatat attgttacaa gtggtatagc aactgatgca
     5821 tttagagatt ttggttattt acgagcaaat ggattaaact tatttggatg tggggctgga
     5881 gaaagttatt ttgccataga tagtaaaggt aaacgacatt cttgtcatga ggctattgga
     5941 aacgaagctt tacaaggaaa gaatatacct caactgggtc ttataactaa agatggaggg
     6001 gatctcctag atatgtgggt tatgcttcac gggggaatgt gtcctcaaaa gaatgatggt
     6061 tatagaataa gtgagtcata tccaagaaag atatatcggt caatagcagc tgatttaatt
     6121 ctattagagt gtgaaagaca ggagaggttt ggctatgaat aaatcataag gaattatata
     6181 tacagtggaa ttaatcataa taaaagggtg aaactgacaa agacaaagtt acaaaaaata
     6241 taaaaatatg acaattgaat gtatgggcag aaaagtagtg acaggtgaat cgacaccatt
     6301 ttcggtgtca atagggacag atgggtcgtg tagacaagtg attacattaa ttggtcgtgg
     6361 tgaaaataat ggtggcagtg cgatatatca cacagaatat tttgagattg ggtctgggtt
     6421 tttagcgcca cgctgctgga cgccacatgt taagagcact gacttcacct cagtagcaaa
     6481 aggagaaaag acgaccctgc cggtaacagg ggcagaaata attggaagtt agtatgcgtg
     6541 attgacaaaa acaaagtaaa atagtggtta tgaaactgat aaagacgaaa ttacaaaatg
     6601 gattagattt ttggtaccga gaggatgata aatttattgg acagagaata gcccttcgga
     6661 aatatgaaga gtatgaaagt atattaatcc ttcgactcgc aaagctcgct caggactata
     6721 gtattgctgt tgatatcggg gcgaatattg gatattacac tttacttctg gccaaagtct
     6781 gcaaaaaagt ttatgccttt gaaccagata aagattgttt tgagattctt aaaaagaatg
     6841 ttgaagaaaa taaattaaaa aatgtgactt tatttaataa ggcagtgggg aatgaaaata
     6901 aaaaagtagg aatagttaag gataaaagta attttggaaa cagtaaaata gttgaaagtc
     6961 taaagtctaa aagtcttaaa gtagaatgtg taagattgga tgatgtgatt aaggaaagaa
     7021 ttgatttgat gaaaattgat gtgcaaggat atgaagaaaa agtgattgag ggggcgagga
     7081 aaattataaa aaaatactcc cctactatat ttatggaaca taacggagga aaactagatt
     7141 ttttgaaaga cgtctataaa aatatttata caattgatta ttggttttat atttgcaaaa
     7201 aaggaattgt ggttgataaa aaaactggct atgctgatct actactaact aacaaaaaga
     7261 taagttttgg gaatagatat aaaaatgtac agtggaaaaa agtgattaag gatataatgg
     7321 gttatgtcta aaataaaatc tatagtagca agagagatat tggcttcggg tggtgctcca
     7381 agcttagaag taacagtaac cacagaagat ggatttgtcg gcgaagcctc agtaagttat
     7441 ggagcgtcag caggaagcct agaagcgaca gtattacttg ataaagatca aaatagatat
     7501 ggtggtaagg gaatgttgat agcagtaaaa aacatcaatg agatattggc caatctggta
     7561 atagggacgg aagtagaaga acaaagaaaa attgatgaaa tgatgattgt tcttgacgga
     7621 actgagaaca aaaataaatt aggaggtaat gctattttgg gagtcagcat ggcagtagct
     7681 cgagcggggg cggcagaaca aaaactagaa ctacatgaat atttaagaaa gatatatggt
     7741 ttagagatag tgacaaaatt accaaaacca atgattgtca tgattgaggg cggtaaacat
     7801 gctgacgaaa caactgattt acaagaattc tgtgtgagtg cgatgggtaa tagaacagcg
     7861 gcagagaatg taagaatgga gatggagatt tatgaagcat taaaaaatat tttaaaaaga
     7921 gaaggtttgt cgataaacgt gggtaacgag ggagcgtttg caccaaa
//
LOCUS       DDWP01000083           12724 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_14023, whole genome shotgun sequence.
ACCESSION   DDWP01000083 DDWP01000000
VERSION     DDWP01000083.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12724)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_14023"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         4432 a         2189 c         2312 g         3791 t
ORIGIN      
        1 cgaccgatca caatcttcgc agtctggcgc taagccagga gaagcttggt gaaggcgatt
       61 gtcttttaat ttttcctgag gggattatca ctggtggtca aactagtcag attatcaaag
      121 gtgggacagg cgctgtgcgt ttgtctttac tttctcatgt cccgattatt cctattggta
      181 ttcgaggtag caatcatgtc catccttatt tattaaagaa tcgattacct ttttatttta
      241 agcgtcatct cccaattgtc atcaaaattg gtaaagaaat taatttccca gcacatcccg
      301 gtcttgattt gtcttctcac actgagccaa atcgccttct tcttcgttct cttaccaaca
      361 atcttatggc caggttttct aaattaagcg gtttgccctt ggtcgacaac tagattattt
      421 ttgacttata attatttatg gtcaaaccac ttctagttat cgtctccggt ccatcatgtt
      481 ccggtaaatc aactctagct cagaaattat ctatcaaatt caaattacct cttgtcacca
      541 aagacggtat caaagaaaca atttttgacc atctcggttg gagcgatcgg gagtggtcca
      601 aaaaagttgg tgctactagt taccggctta tttcctactt tctcgattca atgttagttt
      661 ccggtaggcc tctaattatc gaaagcaatt tcatgtccga gttggatagt cagccaattt
      721 cagaaaaact aactaagtac ggctattttc ctctccagat aatgtgtcaa tgcgacggac
      781 aaatcttatt tgaaagattc aaggccaggt ccgaatccgg tgtgcgtcat cccggtcatg
      841 gcgatgataa aaattacgat gagtttaagg acatgcttct caaaggcaag cttgaggcat
      901 tagatatcgg tggagagata attactttcg acactaccga ttttaataaa ataaactacg
      961 atccaatatt ccaagtgatt gagaaattga tttaggtcag ttcatccgta taaattcttt
     1021 ttaatacact aatgtatttg ttagtagata acctcaataa gtaaaattcg gcatatcttt
     1081 tagcggtata attaacttat gaacaaaaga attctctact tgattcaaca agcgggttcg
     1141 ttaatgcaaa tgcctcgttc tcataaacgc agtctaggca atacctttga cactgttgct
     1201 tcgcatagtt tccatgttgc tatcatcgcc tattgtattg ctcgtatgga aaaactatct
     1261 catgctgatg ccactactgc tatgatgatg ggtctgatgc acgacctgcc agaagctagg
     1321 acgggggata tggactttgt tgccaaaaac tatactaaag tcgacgagcc aaaagctatt
     1381 gatgatcagt ttgctgatat tgattttgga actgatctta aaaaagttgt tgacgagtat
     1441 gaagatcgtc aaagtattat ttccaagtgt gccaaagatg ccgattctgt tgaacagata
     1501 tatcaagaat gggttttgtc ttggcagggg aataaattgg ccaaccaatg gtttgaaggg
     1561 gactttactc atcgtgttcc catgtttttt actgagtccg ccaaacaaat tgctctaagt
     1621 atgaaagaca gcgatccgaa caagtggtgg tgggctgagt ttgttgagaa aggtatcgac
     1681 tacaaacatc ttaatggtaa aggagagaga taagattgta tttatggtct ctgtaccatc
     1741 aatatcagga tttgaaacag ccaattgaat cccgttgtta gtaagtcagc aatccagact
     1801 attacttttg ttattaggtc aatcattaac cggattctat cattcgtatc tcttaattga
     1861 taatcacttt atccttatag gttcttttgc agtaaaatag gctaatgcca attaacaatc
     1921 gtaagcaatt tcactgaagt tggtagtcta agtcttgatt atcaacatac acctcaacca
     1981 atacgttgag agatacaata taaacataaa agatattatt taaaataaaa atttaatggt
     2041 aaatataaat gaatatgcaa actttatttg gtcggtagct gatactttga gaggcgatta
     2101 taaacaagca gactacggaa aagtgatctt acctttgact gttattagaa gactagatag
     2161 tgttctttca gatacaaagg aaaatgtatt aaaaagctat gaatcccaaa aaataaccaa
     2221 tcttcaagaa gctgatctag tacttaataa aatcgccgga tacaaattcc ataaccatag
     2281 taaatttgac tttagtaaat tgatttcgga cccggataat atcgcttcta atttacgaaa
     2341 ctacataaat ggtttctctc aaaacgctag agatatttta gagaatttta aatttgaaga
     2401 agaaattaag accttagata aagccaatct cctctatcaa gttgtaaaac aatttcagga
     2461 aattaactta caccctagca aaataaataa tcatgaaatg ggttatttgt ttgaagagct
     2521 aatcagaaag tttgcagaag catcaaatga aaccgccgga gagcatttca cacccagaga
     2581 agttatcaaa ctgatggtca acattctttt tatttctgat agcaacatcc tacgtggtga
     2641 tggaattata aaaacccttt atgatcctgc ttgtggaact ggcggaatga tgtcggtagc
     2701 cgaagagtat ctaaaagaac tcaatccaga cgccagactt gaactgtttg gacaagaaat
     2761 taacccagaa tcttatgccg tttgtaagtc agatatgctg ataaaagttc aggacccgga
     2821 ccatgtgaaa cttggtgata ctttaaggga cgatcagtta agaaccgaga agtttgatta
     2881 tatgctttca aatcctccgt tcggagttaa ctggaaaaag gccgaaaaag aggttaagca
     2941 agagtatgaa gatctaggat ttggaggtag gtttggagct ggtctaccga gtattagtga
     3001 tggatctatg ctttttcttc aacacatggt ttcaaaaatg aaacccacta atggaggttc
     3061 tcggctggcg attgtattta atggttctcc acttttctct ggtagtgctg gtagtggacc
     3121 tagcgagata agaaggtgga ttattgaaaa cgatatgctc gaagctattg tagctctccc
     3181 agatcaactt ttttataaca ccggaatatc cacatatatt tgggtcgtta caaatagaaa
     3241 accaacggaa agaaaaggaa agatccattt aataaatgct gtatctttct tcaagaaaat
     3301 gagcaagagc ttaggaaata aaaggcatga aattggaccg gatgatatgg ataagattgt
     3361 tagtttgtat ggagatttta aagaatctaa gttttccaaa gtgtttgata ataatgattt
     3421 tggttattcc caaataactg ttgaaagacc aacggttgat gaaaaaaggg aagccattaa
     3481 agacgccaaa ggcaaaccaa aagccgatcc taaattaagg gacaccgaaa atgtacctct
     3541 caaagaaaac atccagaagt attttgaaag agaagttaaa ccatttgtac ctgatgcttg
     3601 gattgatgaa accaaaacaa aagtcggtta cgaaataaac tttactaagt ttttttatga
     3661 gtacaaacca ctaagatcgc ttgaagaaat caccaaagac attctagaac tagaaaagga
     3721 aagtgatgga ttattaaaaa acattcttga ctagcattat aaccatgact aataaaattg
     3781 acaaaaacaa tgcaggtaca atactaaatt cgtttgtcaa agcaatcaaa aaatcttatt
     3841 tatacagcga tgacatttta ataaatgcta ctaactttga taattttcag aatttatctt
     3901 gtaccacttc cataactggt aactattcag aggtaattga aaaaattggt aaaagtacag
     3961 aaaaaaaaga tttagttttt ggaattttcc cgatagggat gaaaccagta gaatataaaa
     4021 tctctggtga tggtaagaca gagcttttat cacaagatca catatttatc ctaaaatctc
     4081 ttcccctcat tagcgagtct ggaataggtt tatttctagt aacaccaaac attctttact
     4141 ctaatttagg gaaaaagttc attggagcgt tggaccgact tggtatgtca ataaacgctg
     4201 tgtttggttt tccaagtaat ttattttatc cacagactgc tataaaacca gtgataattc
     4261 taattagtaa gaatagaaat aaggatattt ttgttgcaga agtagaccaa agctctgaaa
     4321 ttgataatat agtcgacagc ctaaagaaca atgtaggctc cgcaaatcta aacgaggggc
     4381 tctttataaa ccgaaataac tttacttctt ttgaaaactt caagactaca agcgaaatag
     4441 acaaattaaa aacacaatac aaagagtaca aaagctggaa cctttcggaa gttagcctca
     4501 gcattggctc taccagagaa aattttgtaa acaaaaataa ttcgatatat attccaaaac
     4561 tgggaccatc accagttata tctaacctaa atagagctac gctcaaacat cagaattact
     4621 atcaaatagt tcttaacaaa gatattgttg aggctgagta tttgagaatc ttttttaagt
     4681 caaaaatggg gagattattg ctaagttccc ttcttgtaag ttcatatatc cctcatataa
     4741 ataaatctga tcttgaaaat atacaattac cagtccccga taaggaaaca caaaatctac
     4801 ttatagatac agatctgaag ttgaataatc tagaaaaggt cctagatgag ttcaaagaag
     4861 aactgtcttt gaatccgaat ggggcgaaga atatccaagg tttaatcagt agcgttttaa
     4921 acgacttagg aaaattaaat aaagcagatc agatcctatc tttaatcaga aaaggagagt
     4981 caaagaccct tgaatttaaa caaactttct cgactgatat taaaaccaat aagaaagaac
     5041 agttcatagt taattcctca ttgaaaaata tcgttgcttt cctaaattct gatgggggta
     5101 cattgctagt tggggttgaa gacagtggaa ttattaaagg tatagaaagg gattgtttta
     5161 cgagcgatga taaatatctt ctaaatttca aaaacaatat caaagaaaaa attggagaaa
     5221 agtactatca gtttattgat tgggagattg cagatgttta tggtaaaaaa gtgttgatag
     5281 taacctgtaa ggcatcagag gatccatgct atctgaatgg taaagaattt tatgttcgga
     5341 caaacccttc tgtggatcaa ttagaaggac cggagttagt tagctatatt aaaaaacact
     5401 tcgactaaaa tgttaaagac atatcaaaaa tataaaagta cggatgtggt ctgggtaaaa
     5461 gaaatacccg aagagtggaa ttttagaaag ctaaagtttg tctttcaata cgttgtcgat
     5521 aatagaggca agactccacc tatatctgag tcaggaatac ctttactaga aattaaacat
     5581 attagtggaa aaaaggttcc ctcctacaac actgacaaat ttgtttctga ggatgtttat
     5641 aaaagttggt ttagaggaca tattaaaaag aatgacatat tgataggtac ggtcggctca
     5701 accggtgaaa cgtgcatcta tgacgaaagt caacgtgctt gtatcgctca aaacattgtt
     5761 gggttaagag caggaagtga taaccctatg tatttatatt ttattttatc gtcataccat
     5821 tttcaaaaat atattagtgc ctcaaataaa aatgttatcc aaccaacgct taaagtatcc
     5881 gactttatac aaaataaagt ttatcttcca aactcttcaa cacaaactaa aatagccttg
     5941 tatttggact ataaaaccag ccttattgat aacttgatta aaaacaacga aaagttaata
     6001 aaactttttg aagaacaaaa gtcagtaata acaaataatg cagtaactaa aggattgaac
     6061 aatggtgcgg atatgaaaaa tagtggtata gaatggttgg ggatggtccc agaaaagtgg
     6121 aggatactta aattaaagtt tattggaaaa tcaattatcg gtattactta ctcaccagac
     6181 aatgttgtag atgaaggtaa tgggacattg gtgttacgat catctaacat tcaaaaaggg
     6241 gtgctttcgc ttaaagataa tgtatatatc aacaagaaga ttcctgagac attaaaaaca
     6301 aagaccggag acattctcat ctgttccaga aacggaagta gttccttaat cggaaaaaac
     6361 atagttataa ctggtgaaat aattggaagc acctttggtg ccttcatgac agtctttagg
     6421 agtgaatact ggaaatttct ttctaagttc ttcaattccc aagtttttaa atctcaatcc
     6481 gggttgttct catcgtccac tataaaccaa ctgacaataa atactttgaa taatttattc
     6541 attgccctac ctcctacttt agaagagcaa gaggaaatcg caactcactt agataaggaa
     6601 ctgaaaccaa tcgaaaattt aatccaaaag accaaaaaac aaaattactt tttaactgag
     6661 tatcggaaaa cactcatttc aaatatagtc actggaaaaa ttgatgttag agatgagaaa
     6721 attccagaaa gtgggataaa atacaactat gtctaatata ttaaatgaaa atacttttga
     6781 aactaacatt gttgaaaaac tagtttcaag cggtggttat atagaaggca atcctgttga
     6841 tttcattcct gatctagcac tcgataccaa tcaactattc tcttttttac tcgactctca
     6901 accggatgaa attgaccaac taatcaaggt acacggggaa gatgagtatc aggaccgact
     6961 aatccaacgg ttaattaaag agttagattt acgaggaacc cttgattgtt tgcgaaacgg
     7021 tattactgat tatggcgtcc atcttaatct tgcgtatttc aaaccagaat ctagcctcaa
     7081 tcaggagact aaagaaaaat atgatcaaaa cattatctcc gttactcgtc aggttcactt
     7141 tagcgaaaaa gatcctgata aatcagtaga tctagtattg tttatcaatg gtttacctgt
     7201 cgctactgct gaattaaaaa acccatttac cggacagact gttgaaaatg ctcaaaaaca
     7261 atatatctac accagagatg tccgagaatc actttttcaa ttcaaaaaaa gggctttagt
     7321 tcattttgct gttgatccga atttggtcta tatgaccact caactcaatg gtgaaaatac
     7381 taagtttttg ccttttaata agggtctaaa taacggagct ggcaatccat cggtcgaatc
     7441 cggctaccag acatcgtatc tctgggaaga aatttggaat aaagatagtt ggatggatat
     7501 tattgctaaa tatattcacc tagagaaaaa agaaataaaa accgaaggaa aaattattac
     7561 taaagagaaa ataatctttc ctcgctacca ccagcttgat gtagtgagaa agttggttaa
     7621 cgataccaaa agaaatggaa ctggaaaaaa ttatttaatt gaacatagtg ccggaagtgg
     7681 aaagagcaac tcgattgctt ggttagccta ccgtcttgca aatttacata atgatttaga
     7741 tcaatcagtt ttcgattcag ttgttgtagt caccgataga gtggtcctag acaaacaact
     7801 tcaagatacg atttatcagt ttgatcataa gcagggtgtt gttcaaaaga tcgacaaaga
     7861 ttctgctcaa ttagctgatg ctttgaaaaa aggaattaaa atcatcatta ccacggtcca
     7921 gaaatttcct ttcgtcttgg atcaaattga cgagttggaa gccaaaaagt atgctgtgat
     7981 tgtagacgag gcccacggtt cacaaggcgg agaaaccagt aaacagatga aagaagtttt
     8041 gactttcggt ctacacgaat cggaagaccc cgaagattta gcagaagtcg aaacggccga
     8101 agatgaaatt agaaaatcaa tggcatccag aggtaagcaa tccaatcttt gtttttatgg
     8161 attcaccgct actcctaaag ctaaaaccct agaagtcttt ggtgaagttg gcaaagacgg
     8221 caagcctaga ccattccatc tctattcaat gagacaagcc attgaagaag gatttattct
     8281 cgatgtttta aaaagttaca ccacttacaa aacctacttc cgtttatcca aagaaattga
     8341 agatgatccg gaaataagta aggataaggc taatagagcc attgcccgat tcctctcact
     8401 acatccccat aatttatctc aaaagaccga agtaatggtt gaacacttcc gaagagtcac
     8461 tatgaaaaga attggtggta aagctaaggc aatggtggta actagttcac ggatgcacgc
     8521 tgttaggtac tatctcgaat ttaataagta ccttaaagaa aaaaactata ctgacattag
     8581 aactctcgtt gccttttctg gtactgttat cgacaaagat tccggagctg aatataaaga
     8641 agtagaaatg aatggtttta gagagaaaga actatccgaa aagttcaatg ctgacgaata
     8701 caagatactg ttagtcgccg ataaatatca aacagggttt gatcaaccac ttctacatac
     8761 catgtttgtt gataaaaaat tatcaggtgt agccgcagtt caaacattat caagattaaa
     8821 tcgagttgct cccggaaaag aagatacctt tgtcttagat tttgtgaacg accaagcaac
     8881 aatttacgaa tcttttcagc cttactatga gcttacttct cttgaagaaa cagccaaccc
     8941 aaatcatctt tatgacctaa aacatcagtt agaagaaacc ggaattttct ggcaatcaga
     9001 ggttgacagt ttttcaaaag tattctttaa gcctaaacaa accagagctg accaagctga
     9061 actttacaga ctaattcaac cagccattga aaggtatatt gcacttgatg aaacaaaaaa
     9121 agaagaattt aaaaagacgt tgactgcctt tatcagaacc tatgcttttc tttcccagat
     9181 aatgccattt acagatactc aactggaaaa actttatgtt tttgcccgat tattgagaaa
     9241 tgctttacca agaaaaaata ataataactt ggaattaaat gatgagattg ccttgcagta
     9301 ttacaaactt caaaaaattt cagagggatc agttgagcta tcaaaagaag gggaggtaaa
     9361 tctccccgga ataatgggtg actctggtaa tgcttccact ggtgaggatg aaaaatccaa
     9421 actatcagag ataatcaaaa cattaaacga taggttcgga actgagttta gtgaagcaga
     9481 taaacttttc tttgaccaaa ttgaagaaga gttaatcgca gatcaaaaac tccaaaaaca
     9541 agcattaaat aacgagatcg gtaattttaa atatggtttt aatgaagtat tcgtcgataa
     9601 aatcattgac cggatggaac agaatcagga aatctttaat aagattatga ataatcctga
     9661 ctttaaagca gttgtttccg attggatgct caaaaaggtt tacaaaaaac ttagtcaaaa
     9721 agtatcaata aaatccttaa ttaaagaagg tgaaggacag aatcttgaat ttaaatcttc
     9781 tttaaggtgg gatgttaagc aaggtaaaga aaacaaagat ttggaaaaag tgataatgaa
     9841 aactctcgcc ggatttatga actccgaagg tggacagcta ataattggag tagaggataa
     9901 tggcaatgtt cttggtttgg aaaatgatta ccaaactctt caaaacggaa atagagataa
     9961 gtttgaaact catgtggtcc aactaatcaa agcctcattg ggtatagagt ttgttcaata
    10021 tgtcaggttc ctgttcgaca aaattgaagg aaaagatgtt tgtttagtaa ccgctatcaa
    10081 atcaccaaaa cctgtatatg tgaagtatca agacaaagag gagttctttg cccggaccgg
    10141 taattccact agcccattta ctctaagtga agcacaggag tttattagaa ctcattggaa
    10201 ttaaatagaa tgattgccac cgcttctgct acacctgcca ttattaaaac agttgaagaa
    10261 gttaccactt catatccatt gtgggctact atcgcaaaag actttggaat gccaggctta
    10321 ataggtaccg tgctcggttc gtttacaaca ctttctattg agctattaaa aagaaaattc
    10381 tctactaaag acgaagaaag aaacaaaaaa ctgaaagtat atgccaagtt aaacagtcta
    10441 attttctcaa caaaacagct atataagtcg aggtttgaag cgtcaataca ttctgactat
    10501 catgaggcga aatggatatt cgccggacac aatgaacatt caattgatta tgatgaagct
    10561 aaaagatgga tgcttaagtg tgaagatatg gttcaagaaa ttatcaagaa caccaaagaa
    10621 ttacatatgt gcttagctac aatcaaagta ctttttaata aagatactga tctagatccg
    10681 atgatcagtc aaatatattt aatgaacggg ataacaatac aaccacttaa aagttcacca
    10741 actcttgacg atattgaaga atggaaaaaa atttcaaaac aggatcttga tagccagatc
    10801 aatgagaaaa tttctctgcc tctaactcaa ttgaaaacga tcttagatgg caaattatgt
    10861 gttaactagt tggtcagttt ttatctatta acgattgctg gattgcttct acatcaagaa
    10921 tatcaatttt gagtacatcc aagtttgaat ccaaatgaac aatgcttttt actttcataa
    10981 gtctcccttt gtcgattaca aatggataga atctttctgg tctttcatta ggaagttggt
    11041 atagtatttg ttttccattc gtcaaagaaa atttccccct gtttgtatgt ttgttcaacg
    11101 aaacaaattc accttgtaat tctgcttcct gggttttagc tatttcctca acattaccat
    11161 caaaatacgg tttttcctca tctgctattg tggttgaaat tgttttacta tctaataaag
    11221 ctgtaacatc caaagaatct atcttacctg tctccaaagg atttactatt ttgcttaaat
    11281 ccggaacaat ggtcttttcg ctaaagatgt taaatacctc taagggaaat tccttttcta
    11341 caccatctga attaacaaca attactacat ttccttctcc aattttcgtg tgtaagggtc
    11401 tgtttttagt atgtttaatc atgtcgatta caccgaaaat tatcttaatt actcccacag
    11461 ctccgccagc tacaacaccg acagcttgta tttgatcagt attacttgta atggcctccc
    11521 atacctgaag tgaaatatca aatgaaccct gggttaatcc ggttattctt atcttgtgtt
    11581 cgtatggtaa gtttttcgta gcaacaatct ttgaatacgc atttgaaaat ccttgtaaag
    11641 ctggtactac atcttctact agcattgtcc catcatctac atcgggacca tggtatttca
    11701 gtgaaacatc tacaatttga gatgatggca tatatataaa tttacactaa ataaataaaa
    11761 gtaacactgg tatcatttcg gttctatttt gaaaaacaac ataatttgaa taaatctgta
    11821 tcattccaat tttttacatc atcaaactgc acaattcatc cagaagtagt tccagttggc
    11881 gagtattcca ttcgtaaatg taaccctatt atttccagta aggaatcgaa aaatcagtat
    11941 acccagaatt tgatcgctaa aagaaaaagc ttggttaaac caaaaatgat aaggtgaatt
    12001 atgtatggca acggtgtgca ataaattcca ctaccgaaaa aagtgactaa tagataaatc
    12061 gttcctgctt gaaataatag atatgcccca cttcctataa atctattaaa tgaaggtgag
    12121 aatccgtttt tggaattata atgccacctt ccgtctctag tgaaaatttc aaaaattacc
    12181 cataaaacaa gcatgattgt aatccatatc cgatattgcg accagaaatt tgttattaaa
    12241 aaccaaatct gtcctgcatt tgattttggc gggctctcaa ataaaagttt acatatttgc
    12301 ggtggtaagg ttgaaattgt ggaattattc attctgtgac attataagac tgactgatct
    12361 gttttgtata gttttgttag ctatatctta aaagtagcaa ttgcagatat tttccatctc
    12421 gtatagccta atttccatcc actgcgttcc atatctccat gatggcgggg agcggcgtgt
    12481 ataaacttct agtataatcc aaccatgtct cccctaaaat tcataacaac cccaggagag
    12541 ccgagcgtag aagataaaca aaaattagtc gatggtatgt tggcgcacca cgccaaaaat
    12601 ggtcacatca gaaagactga gactatctct atttttcttc acgacgaaaa tgaccaagcc
    12661 gtcggtggag tgatcgtttc ttttctttgg ggtggtatgg aaattcaagc actttgggta
    12721 gatg
//
LOCUS       DDWP01000084            3186 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_14380, whole genome shotgun sequence.
ACCESSION   DDWP01000084 DDWP01000000
VERSION     DDWP01000084.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_14380"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          945 a          807 c          415 g         1019 t
ORIGIN      
        1 cctttatcat cgtttaaaaa acacaccaaa tgaataactc aaaaatatcc ctcaccattc
       61 tcaccaaaaa cgaatcagat gtcctaaaaa agaatcttaa ctggatagac caatgcccag
      121 caatcaacga aataatcttc gtcgacgacg aatcaaccga cgacacactc aaaacaatcc
      181 gttctctcaa attcaaaaac tgccacatca aactatttga aagaaaacta aaccacgact
      241 tctcctccca acgccaattc gccatatcta aaaccagcaa caatctagtc ctctggcttg
      301 acccagatga acatccaagt aacgaaataa ttggattatt aaataatatc gacaaacatc
      361 aatatgataa ttacgctttt cttcgctctg atatattcct agataaagaa ctaaaacacg
      421 gagaaacttc atcccaatac ttcctccgtc tgtttgacaa aagatatggc caattcatca
      481 atcaagtcca cgaaacttgg caaagttcaa aaccagtcag aaattttgac ttccccatta
      541 tccaccatcc acactccagt cttaaaacat ttattgaaaa gataaatttc tacaccgaca
      601 taagatcaca agaattacac aacaggggag tcaagacaaa cattttacaa attattttct
      661 atcctctagc taagtttatc caaaactatt ttcttagact cggttttctc gacggcacac
      721 caggaattat tatcgctcta ggtatgagtt tccactcatt tctagttcgc gccaaattat
      781 ggacactgca aaatcaatca tagccatcag caccgccatc gttatgttca tgggccagct
      841 tttaaggcta gacattgccg gcatctcatt tccagtcatt gatttattta tcttcctcct
      901 tttcctaact actgacaaat caaaaatcac caacaaacct tttttatttt ttatcatctt
      961 tgcttggata tctctggcta taaaccactt ctttggtcta ggcaccacac catccgcctg
     1021 gatgtattta attcgattaa cactgttaat tggcctgata atctatccac ctaaattaca
     1081 acctatcgcc aaaaacattt tacttttgtc attatttgcc aatattatct ttggcttaat
     1141 tcagtattat ttctggccag atttcactta tttcaagtct ttaggctggg acgaccacct
     1201 taatcgacta gtcagtacct attttgaccc tactttcacc ggcctaatct atcttatgtt
     1261 cttcctctac ctctacttta acttcctgaa gtattcacca ttaatattta tccccattgc
     1321 tctcacttac tcccgttcaa catttctatc tctatttatt tctttattct ttatcaccct
     1381 taaaaccaaa aaaatatttt ttcctgccat attgtctttt acattttgtg ttttgatttt
     1441 attacttcct cgaccagccg gagaggggac aaagcttgaa agaacatcat ccattagcgc
     1501 caaaattgtt aattaccaac aagctctcga tactttcgtt aagtcaccta tcattggcca
     1561 cggctacaac aacctagcac tagtacgcca cacaggagct gtctcaagat ctggatttga
     1621 tagtagcctc ctaactgtcc taactacaac cgggataatc ggctttatac tttttgctct
     1681 tggcctattc aagctattta aacaatcatc actcttaaaa caaaccatgt ttgttgcaat
     1741 catcattcac tcactctttg ccaactctct tctctatcct tgggtattag tcttattcgt
     1801 acttctctga agtcctaaat atcgtaagtt atttctatct ttttctttgt ctcgttaccc
     1861 gccaggtctc tgactattat ctctacttca tttttaccaa ttgccaattg tagcttcaat
     1921 ttaaatgatc cagaatcatc aaccatggcc actctgccat taactgttac actcacaccc
     1981 ttctcgctaa cacccaccac atcaaagtca tctgattcga ctttaacaga cgcttccgaa
     2041 gggttagtca tagttagggg aggaacctca ttatcatacg ccagggtcag ttctttggat
     2101 atttcactac tatcaccagc tttactaata gctactgccg taaagatgct ttcaccggcc
     2161 tctaagaata tatctttaaa cacaaattca cctgcttcat cggtttccac tttacctacc
     2221 gaaacatcat ttttgagcaa ttcaatagac accccttttt ccgccacccc tctaaggtca
     2281 attcgggcac tgtttgtcgc ttcaaagggt aagattaatc tcggcatcat tgggggaata
     2341 actttctcta ctatttcact atcttgttta ttttttagat caccaaggag tatagaaaat
     2401 ctaacaagta gtggtaaacc aaaaacaacg gttagcacca ataccagcac tgttatcaaa
     2461 ccaagaaaaa ttgttttttt ggttatttcg tcctcagctt tcttatctaa tctagagcga
     2521 gtaaattctg ccataaacta tatctattct aacatttttt gcgacatcat tcagccacca
     2581 attaatttta tccaaaactt caaacaatta ggagccgaag gggagaatcg gactcccgac
     2641 ccacgcttta cgaaagcgtt gctctaccaa ctgagctact tcggcaaaca gttaggtgag
     2701 cgggtgatgg gaatcggacc cacttctcat ccttgggaag gacgcattct accggtgaac
     2761 tacacccgct tcaaaactat tctatcacgc tagttagttc agcttgggca acaccagagc
     2821 cattccaatc tccaatttat ccgggctaga taatttactt ttattatttt gccagatcat
     2881 tacccactta tatccatcac catatttatt aactgcaatg ttccacaagc tatcgccacg
     2941 gacaactgta taagtacttt cgtcaacaga aacaccctta ctatcttgga ctttttcttc
     3001 agctacagta tctttcttta ccggctcagc cttagctctt agctttattt tttgaccgac
     3061 ttcaagagta gttgttttta attcattatc tttaacgatt tctgtccatc taaatccatc
     3121 accataatta gctaccgcta tgttccataa actatcaccc ttcttaacca catattcacc
     3181 ttcaac
//
LOCUS       DDWP01000085           20577 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_18305, whole genome shotgun sequence.
ACCESSION   DDWP01000085 DDWP01000000
VERSION     DDWP01000085.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 20577)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20577)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20577
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_18305"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    6787..6967
                     /estimated_length=181
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7360..7434
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7046 a         2875 c         4777 g         5623 t
ORIGIN      
        1 gggacagaat cgtttagtgc tacgagaatg atgggtggaa tatttattgt aggggcagtg
       61 ctaatactcg gtttttcaaa tgctaaaaaa aataagttta aagtaaatag atacttttgg
      121 atgttaatgg cagccacttt tttctatgcc atggctgcgt tgatagataa tatagtgatc
      181 tcacagggac gtttttcacc gctgtttttt caagctttaa cctttgggat accttctttg
      241 ttgatattgc ttataaataa aaaggccata gagcatctga aatacgtata tcatccgaaa
      301 gtatataaat atatattaat taatgcggtg ttcttctttg ccagcttttg ggcgatatac
      361 aaagcatatg aggtgggagg agtgacatcg gaagttaatt ttgtagcgtc aacagagaca
      421 atactaacgg ttgtcttggc tagcgtctta ttgaaagaaa aagagaatct atggataaag
      481 gtgttttgtt cgatattggc tggaatagga atatttttac tgatataaat agacatatgg
      541 aattagaaaa taaaacagcg ataataaccg gaggaaactc gggattggga cgggaaattg
      601 tcaaagaatt aaaaaagaaa aattgtaagg taattgtggt gggtagaacg gaatctgatt
      661 tgacatgtga cttgagaaaa tacgaagaga taaaagctct gggaaacaag atagacaaag
      721 tagatatatt aattaactgt gctgggataa tcgcctacca accgctagaa aaacatgacc
      781 cacaaaatat tgccgatgtg attaacacca acctgctagg aactatctac atgacacaaa
      841 tggtactgcc aaagatgata gcccaaaact cgggaacgat attaaacgtg tcgtcaactt
      901 ctggactaat gactggcgga catagtggtg agtcggtata catggccagt aaatacgggg
      961 tcagcggttt tactgaagga ttaaaaaaag agatggagac agataaaaga aatattaagg
     1021 tggttggatt ctacccgggt gggatgaata ctaagttttt ttctaagagc ggggaagata
     1081 aggacacctc aagctttatg gatccaagtg aggtggcgaa aataattgta tttttattag
     1141 agaggccgga ttcgataaac atcgaccaca tagtagtcaa taggaacaaa aatattgcag
     1201 ggtaaggagc ggtaagagct ggagtagaat atggtgtgtc aaaactaaag aataatataa
     1261 gaattgccta tatattatcg tttctgagtg aactttattt tccgatatct gtctggatat
     1321 tttattattt gagatttttg gattttaaag aaattggtat attaacagcg gtaaaactgg
     1381 tctcgtctaa catatttgaa gttccgacgg gcgtgtttgc tgatgctttt ggaagaaaaa
     1441 aatcaatagt tatctctttc tttttatata gtttggtaat gtttggtttt gctaatgtat
     1501 cgatattctg gatgttcata gtgttggata ttttaaaggc tttgagtaat gcctttttct
     1561 caggaagctt ggaagcattg gtttatgatt ctctaaaaga gaatggggaa gaaagtcgat
     1621 atgacaaagt ggtggccaat atggaatcaa tagtctggat aggacttttc ttatcggcaa
     1681 tcgcaggtgg ctatatgtat cattattggt ttaaatctcc atatgtagtg caaggagttt
     1741 tatatctaat ggcagggttg gtggcaacaa gattagtcga gccgagaact gattcggaaa
     1801 aacataaatt gtcggaaatg gttaagttca attttgttgg ttttaaagaa ctatttgcta
     1861 atgcaagaat gtcacaaatt tcaattgtat ttattactgt aggagctgga tattttattg
     1921 cttcagagat gttgggtatc tctcaagcac tggagtatgg tatggactca agaggggtag
     1981 gattattatt tggagctgga tatattatat cggctgttgc ctcacaattt tatccaaaat
     2041 tacgaaagtg gctaggagct agaaaacttt tattaattac atctggaatt ttgataggat
     2101 cattcttgct ggcaaaatgg gtgggggtaa tcattggatc aatattaatt gtgggaagaa
     2161 tttcaagctc aacgacattt agaaatactc gatcgagcat tatcaatgga ctaattggct
     2221 caaaaagtcg ggcgacgaca atatcgacgc tggtgctact gtcacaactc ccgatggcct
     2281 tactcgccta tttcatgggt gattatattg acagaaactc accaaattct ttggcttgga
     2341 tactaggaat agtaatgata ataatcttgg gtggccaggc agtgtggttt aataaaaaac
     2401 caacagaaaa aaatgtgtac aataaataga tgattgctat taatataatt tcttatcttc
     2461 tgtcttttct ggcaatatgg tttggggcgg ggttgattgt aaaatcaatt gaatcaattg
     2521 ctcacagatt gaagatgtcc ccttttgcga tttcttttct aatactgggg gtaatgacat
     2581 cgataccaga ggcggcggtg gggataaatt caattgtcaa caatgatcca gagatatttg
     2641 tcggtaatct tctgggcggg atagcagtga tatttttgct agtcatcccc atattagcaa
     2701 tattgggtaa agggataaag ctggatcata gcttggataa agataatttg gtgggcttac
     2761 tgcttgtaat agttggacca agcctaatag taatagacca tggagtgacc aagtttgaag
     2821 gggtaattct aatactgtgt tacctaatat cgctatactc gatattaaga aaacatggct
     2881 tctgggaaaa gaaagaggtc gaagaacttc acaagaggac gatatctttt gtggagttag
     2941 ctaaggtagt ggctggggta ataatagtat ttgtatcgag ccaatttata gtcgataaaa
     3001 cacttttgtt ctcggaaatg ttgaagataa acccatatta tttgagcata ttggtgctgt
     3061 cattaggaac aaatttacca gaactgtcaa tagctattag ggcagtaata tcgggtaaaa
     3121 aagatattgc ctttgggaat tacttggggt cggcggcggc aaacacccta ttatttgggg
     3181 tacttacaat tatgaacaat ggagaagtgt ttactttcaa tagttttcta ataacttttc
     3241 tattcattgc ctcaggactg gttctgtttt atttcttttc gcaaacaaag agagatatct
     3301 ctagaaaaga aggttctata ctgctgggta tttatgttct gtttgtgatt tatcaaataa
     3361 ttagtggaat gagaagttaa gatgatgagt tttacacaaa aggtgtatga agtggtgaga
     3421 aagatttcaa aaggcaaaac aatgtcttac aaagaagtgg ccatattggc aggtaatcct
     3481 aaagcgtatc gagcggtggg aaacatttta aataaaaact atgaccctag catcccctgt
     3541 catagggtaa ttcgtgcgga cggaaaaatg ggtggatata atcggggaga agacaggaaa
     3601 aaggaaatat tggaaatgga aagtggagta aaatgactat atgaagaagg aaatgatagg
     3661 aattacagga atgagtggtg ctggtaaagg gacagtagtg gaaatgttga ctaagatcgg
     3721 gtttaaacat ttttcagctt ctgagcttct tctagaaaag gtaattggaa aaaatctacc
     3781 tcaaaacagg gactctctga ttgctatggg taacgagcta agggggaaat atggcccggg
     3841 gtatgtcgtt gaagagttaa ttagaagagg acaatcaacg acaggtggtg tggttataga
     3901 aagcgttagg actataggag aagtggaaac cttaaagagg gctggtggag ttctcttggc
     3961 tgtcgatgcc agtcaaaaaa ccagatatga aagagtggta aaaagaggcg ggataaaaga
     4021 tcaagtctcc tgggaggagt ttgtggaaca agaagataga gagtcaagat cagaagatcc
     4081 taacagacaa aatctaattg cttgccgaaa cagggctgat tttgtaatat gcaatgatgg
     4141 aactatagga gagttagaag ctcaagttaa tagtttttta aaaaaagtat gacaaaagaa
     4201 gcagagtgct gtccaaaatt tgatccaaaa ccatgggatg ggaaaagttt tgaatggaaa
     4261 aataagaaat ttgtaaaaga taaagtcttt tgcttgttct acatacctct taattttgga
     4321 caagtaatgg ccaaattgat gggaaaagtg gacaatgggg cgatgtgtct atcagatcaa
     4381 acatcaatgt ggagcatgga cgtatatatt gaagtaaatc aagaaattga agggatggat
     4441 aacgtcactt taagtggcaa gtacatatca aaagtttatg aaggtgaatt caaagatgta
     4501 ggtaagtgga tgacggattt taagaaaaat aataaagtag aaaaagtata tatgtggtat
     4561 accacctgtc cggcatgtgc taaaaaatgg ggcaaaaatt atgtggtggc agtgggagaa
     4621 ataaggtgat tatttccaag catcgagaga cagcttagcg tcggaagggg aaatgacttg
     4681 gatgataata cccatctgga caagaacata gtcaccctct tttacaggga taccagcaac
     4741 caaaacttgc cgagattcgt gaggatattg aacagttgcc tgctggcctt ggagactagt
     4801 gattttaccg ggaattgcta agcacatgct aagattatta taatggaaaa gaagaagaaa
     4861 ttagtagttg gatggttttc atttagttgc agtgaagata gtacgatatt gttcactgaa
     4921 ctactaaatg aacacttgga cgaatggaaa aaggtaattg aatttcgcca tttaaaaaca
     4981 ttaaagagta ataacagtat cgaaggatta gatgtagctt ttgtggaagg ggcaattgcc
     5041 tcaccaacgc aagaaaaaga actaaaaaag attagagaaa attgtaaaag cttggtggcc
     5101 attggttcat gtgcttgtac agggacacca tcaaactgtc gaaataagct ggtgccggaa
     5161 gaagtaaact ataaaataag ctggtacttg agtaattttg actatagcaa agaagttaag
     5221 aagctggccg atatagtaaa agttgatgac gaagttaatg gctgcccaat gaacgcagag
     5281 gttttcttga aaatcatgga taaatattta aaaaagtatg catagtggag agataacaat
     5341 tgatagtatt actaagattg agggaacggc gggactgtcg gtaacgataa aagacgataa
     5401 agtaatagat ctaaaattca tgattaagga ctatcggagg ttttatacaa gtactgtcaa
     5461 gggtaaacca atagtggcag taccgtcttt tctgtcacga atttgcggga catgttctat
     5521 cgctcatctg ctgtgcgcga tagaagcaat tgaaagatca caagatataa aaatcaccga
     5581 acaaacaaga ttgctacgcc gcttggcata tgatgggttg atgattcgag atcatgcatt
     5641 acacttatat ttttttgtat taccggacct attggggatt gactctatat tagatatccc
     5701 agatgatcat gacgactttg ggcatcagct actacacgat tcttttgata taaaaaaggc
     5761 cggctctgag ctatcaacaa ctatcgctgg agctataatc catgcaccga tgccaacaat
     5821 aggtggattt atgagcttac cagatgtggc aaagtttccc gaacttatca aacaacttga
     5881 aggtattaga ggacaggtta ttagaggagt gaaggcattt gcgggcatga aagacgtgct
     5941 gattcgtaat tctgactacc tagcgttgag aaatgataaa tattttgatt ttctggatgg
     6001 cgaaataatc aactcgagtg gcaaaaaaat taaggaaaca gagtttcata attttctgga
     6061 ctcagtggtt atcccctatt cgcaagctca tggatatgtg ttctcggacg ataagcaaga
     6121 ctacttggtc gggtcgctag ccagaatcaa tttaaacaaa gattcactaa ataagagaac
     6181 gactgaatca atagaagaat atctagcact gttcccatcc gacaatgttt accacaacaa
     6241 cctggcccag gcgatagaaa tattgcagtg tgttgacgat gcgattgaaa tactgtcaac
     6301 aatagaactc gtacccgaac aaccgatcag ggtgccacca cgaccagggg taggcgtggg
     6361 ggtgatcgag gcaccacgag ggacacttta tcatatggtg gaagtggaca atcagggaat
     6421 agtgattgat tatgatgtga tagtgccgac agcccaaaat caaattaata ttgagaacga
     6481 tctaaaaaaa tactttgaag aaaatctaga taagggtgaa gatgtgctga agttggaagc
     6541 agagaaaatt attcgaactt atgacccatg tatgtcgtgt gcaactaatt ttttgaaaat
     6601 tgagtggata aggttgtagt ctaattcccc ctggggagga atgccgataa tatttacttt
     6661 gtctagcttg ccgagctttc ggagatattt taattggaag ccaaggtcat aatcatgggc
     6721 ggtagtgcgg ggtgaagaaa taagctgatc gatgtttttt tcagttaaaa gggtagggac
     6781 gggtagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnaac acggcagaaa gcagatttag aaaatgtatt ttggaagata atggtgacgg
     7021 aattgaagaa gaaattagcc attttattgt gttaaaccca tatttggaga cgttaaaaaa
     7081 aataacgggg ttagataaat tctcctatga gattgctgaa agttattggc taggaaatga
     7141 agaattaaat aaagccacga tagaggatta tcaaatatta ctggataatt ttgttaagca
     7201 aggagtcccc gactggctaa tcgaagagtt aaggcagaaa acacccaaga aatttatccc
     7261 gacacattta ttccaagtat tgcatgtggg agtaggacgg gctagtgggt cggtgccatt
     7321 taatatgaaa acaatcaata attgtattgt gagatggggn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattaaa
     7441 tccgggaata gagaatgggg atgtggtggc agtgcactgg ggaagagtgg ctaagaaact
     7501 gactccagag gaagtggaga agcttgagta ttggacaaga gtggttttag agaatataga
     7561 aatagacaaa aacctagaaa gatagttaga ctggggagag taaatagcac cagccaatta
     7621 tgagtaacga agataacgga taatagatta gtgattagag tggaagcaac tagaactgag
     7681 gtaactacag tcaccggagc aaactttaga gcacgatacc aggtagtagt atatagaaat
     7741 aaagtaatga tggtggcaga caaccagaac agttgtgatg aagagagtcc aggtaaattc
     7801 aatatagact taggataaaa aattatagaa gcggtcagta gcataagtga accaatacct
     7861 aagcgagcgg caactacgag atctgggtcg actgtcgata aaatcttttt tgccaaaata
     7921 ttttctacgg cccaaaatat agtagcaacc aaaatcatca attcgccgat agaaaattga
     7981 aacttagtaa aaccaccaat aaataaattg ccaaaaaaga gtaaggtggt ggcaatgatt
     8041 tggagtcggg aaagtttttc tttaagtagt gggatagcta aaatggctac ccagacaact
     8101 aaggttttat gaattaaagc agcgttgatg gccggaacta aggataagcc agtgaaataa
     8161 agataaaatg gcaggaaacc accaacaaga ccaattgaga gtaagtattt tatttctcgg
     8221 gattttagct ttttaattag cgatatcttt ttagaaaaga tgattagggc gaaaactaaa
     8281 agagcgacag cactgttttt gacagtggta aaaactaaag ctggctgaat ggcactgacg
     8341 gcatatttat taataaagat agaaattccc gaaactatgg tagtaaggat ggcaaaatga
     8401 ataccattaa ttttcatact taatattttt cttgataaat tctgtcggca ggacagccgg
     8461 cctgagaaag gattttggcg gcttcatcga ccattgattt gttgccacag atataggcac
     8521 tgcaatttga agcatcagca aattcttttt tgacgtaatc agtaagatgt ccagtataac
     8581 cagtccaact gtcccctggt tttgataaaa ctaatttgta tttaaaatta ggatatttct
     8641 tttctaattc tgtgaaatga ttttgccaaa agatgtcatc ttgaaaacga agtccaaaat
     8701 aaaatgtaac gggtttgtcg actttatcta aaactgattc aagaatacaa cgaagagggc
     8761 tacagccaga accggtacct agaaaaagta tattttcagc cccatcgtcg ggtttaaaag
     8821 taaaatgacc aaatggacca cgataattta tggtgtcgcc aactttaagg ttttcaaaat
     8881 aaatagaacc aagaccacca gggctagtat cgacgagtag ctctatcttg tcttggtcga
     8941 tggttccagc gatagagtag gcattgattt tctggtcgtt gatcttgata gtgataaact
     9001 gacctggatc ataggaaagt ggtgcttctg gcttaattag aaaacggtgg aatttggtgg
     9061 taagaatctc gtgagaaatc actttggctt gttgaaaact gggaggtgtc ggggtaacga
     9121 taggtgcttt ggtaatagct ggctgatctt taagaataac tttagcgtca aggccaaaag
     9181 tgccatcgta agtaatgacg acaggattta tttcgacttc agaaatctca ggattagact
     9241 cgacgagcag gctcaaacga accataattt cggttaactg gctaatatca tgtggtttgt
     9301 caccacggta accgtttaac agaggataaa cttttgattc tgatatgagt ttcttggcgg
     9361 aatcagtggt tataggtaac aggtgaagat ttctatcgac caccaactca gcgagtttgc
     9421 cgccggctcc aaaaagcatg actggaccaa aaacagaatc gtttttgaca ccaacgataa
     9481 cttctattcc tgaaccaatc tgttgttgga tttggatagt aaggccttgt ttaacttctt
     9541 cgtctaaatt ttctacagcc tgaccaatct tgttccaggc tacagagaat tcctcaaaac
     9601 tattgatgtt ggtaataact ccaccgacgt cggctttatg taaaagtttg ggtgaagata
     9661 tctttaagac tacaggaaag ccaattttcc caacaaactc ttgggcttgg gtaatatcca
     9721 tggtagaaag tgaagcaggg acagggatgc cagcagaaac gataagatcg gtggcagaag
     9781 aaagagattt tttgattttg tcttgatcta aaacaatgtc tttggtggca ggtacagttt
     9841 cagactgggc gtttcgccaa gattgccagg cccacatgag agaaattgtc ttgatagctc
     9901 tctcagggaa agggaaggag ggaatacgac aatcattaag aactttttca cccgaagcag
     9961 tgactccccc gccaataaaa gaacaaagaa tcggttgggg gtattttttg ctcatggcgc
    10021 cgataatttg ggcggtcttc tcgatctgag tcattaattg aggtgtcaga ataaccacta
    10081 ctgattggac ggatttttct tgaagaacta tttctaaagc ctgaccaaaa cggtcggcca
    10141 aagcatcgcc taaaacatca acagggttga caaaactagc catgcgagga agacactcag
    10201 atagtttttg cttagtatct tcactaaatt cagcgagttc aagaccaaga ctattgatag
    10261 tatcggtact caaaacagct ggaccaccag cgttggtaat aacggcaact tttgggccga
    10321 gaggaacatt ctcccaagca aaggtgcgag caacatcaaa aaaatctcct aactcagcac
    10381 agcgaataat accggcctgt tttaaggcag tatcgagaac attgtcttcg ccagcaatag
    10441 caccggtgtg agaatgcatg gctttgaggg caccggctga ttttccaggc tttaacatga
    10501 agattggatt tttgagggaa atttctctag cggtcttaat aaattcaggg ccattagtga
    10561 tagattcgag gtaaagaccg atgggaaaaa gtttggaaag accggcattg tcgggaattg
    10621 gagctggttg ctgagtcgac caaaatttaa gtatgtcgtt ttcactaaga acggccttgt
    10681 taccgacagt gacaaaatct ttaaaaccaa gcccagtggc ttcgcaccag tcaaaaagag
    10741 cggccgcgat agcaccggat tgagaaataa gacgtagact accattgttg gtaatcactt
    10801 ttccaaaagt gacattaaca ggggtgtcgt tgttggcaaa accaagacag tttggaccaa
    10861 gtagattaag ttgatatttg ctggctatgg caaccaactc ttgctctaat ttgactccct
    10921 ctggcccgat ttctttgtag ccggcggtat aaactagaac attcttgata cctttttgac
    10981 caatttcttc aagaacggta ggaacaacag aagaagggac agcaataact gccaagtcgg
    11041 gaacttcagg aaggctcgta acgttggggt aaaatttgag attaccgatc tgggtgacgt
    11101 tagggttaat tggatagatc ttacccctgt acccagagag gataatattt tttaaggtga
    11161 ttgagccgac tttctcgggg ttttcggaag caccaataat agctaaagat tttggagaaa
    11221 aaagtccagt taaatcacga gtcatagtat tagattaaca aaaaaaagac tactcgtcgc
    11281 gcagaaagat cctgataaga tttaaggcaa ggtataattg atgatgaaga acattccaaa
    11341 tgagatacaa gctgtgttag agaagatttg gacagagaca aatccggagt cgatgttttt
    11401 gtatggatca agtgcgaggg atgactttga tgaggcgagt gattttgagg tcggattggt
    11461 gtttcaaaaa gaaaaaaagg ttcccagaag tgaactagca aaatatcatg atttcaaaaa
    11521 tttaaaactc tatccttttg tattagaaga gttggaagtt ggaaaaattg atacaccatt
    11581 tcctaaagca atatatttaa gtagtttaag agatggagga aagacaattt ttggaaaaga
    11641 agttagagag attattaaag aagtcaagat agatatgata gatttagtgg aaagcttggg
    11701 gttttcttta ggaagagcat attgtgcagt agtgtcgtcg agacaaaatg acatggtagc
    11761 agtacaagag aatttcagta aagcagtatt ttatggactg caaataaaga tatttctgaa
    11821 aacaaataaa ctagttcatt cttacgaaca gattgttaaa gaaataggaa ttagagaaga
    11881 tatagaacta attgaaaatg cgatgaaggt gagaaaagat atcagtagca ttgagattcc
    11941 tatgctctac agagcaatta gttttctaaa cgaaattctg gaagaggtaa gaaacggcga
    12001 aacttcggat gtttgatatt tgacttcggg taatattcgg tatataaata attaagttaa
    12061 taattctttg gctatggata caactaagat tgctttgttt agaggtaaag aagttagaag
    12121 tattgttttt caaaatgaat ggtggttctc gattatcgac gtgataagag tgttaacaga
    12181 tagtgataga ccaagtgcat attggacagc tatgaaagtt agagtaaaaa ccgaagatgg
    12241 atttcagtta tctacaattt gtagacaact gaaacttgtc agtagtgatg gtaaaaaata
    12301 tgatactgat tgttcaaaca tagaaggtat ctttaggatt atccaatcaa ttccatcacc
    12361 aaaggctgaa ccatttaaac gatggctggc taaggtaggt tatgaaagag tccaagaaat
    12421 tgaagatcct gaactgggat caaaacgaga aaatctacgt gaccatatgg atgactttga
    12481 attaatcttt acaatgttag gcgagagatc aacaactgaa atccataagg tagaagattc
    12541 gaaaggggta attaagctta aagatgatgc caatcgtggt ggtagaattg ccggaaatgc
    12601 aagaaaagaa ctagaaacag agctaaagag atcggtggtt tcaaaaaaga accaactgaa
    12661 aattaaagga taataatggt agaatgtacc catgattttg gggaaaaaca tacattatgg
    12721 atttggaaag gagccgatat tcaatggggc ggatttttac ataggtaaaa accagaaagt
    12781 aggactagtg ggagcgaacg gggcaggcaa atcgacacta tttaaattaa taagtggaga
    12841 agactcacct gaagagggta agttgactat tgatggaaca gtattgtctg taccacaaga
    12901 agtaaaatac gacgcagaga tggagaatgc ggagtcgatt agaaaatact tggatcctaa
    12961 aaatttaaaa gaagactacg agctatcgag aattctggca aaattggaac taggacaatt
    13021 tgacctcgag agtcccccac taaatatgag cggtggacaa aagacaaagc tggcaattgc
    13081 cagagcactg gttgctgagc cagacatact attactagat gaaccgacaa actttctaga
    13141 cactgctggt aaagaatggg tgatgaactt cttgggaaga tatccaaaaa cattaatagt
    13201 aatttcccat gacttgaatt tactagatag taaaatagac aagatattgg aaataaattt
    13261 acagagtaga aagattgatg agtatgcggg taattacacc gcctatctaa aactgaaagg
    13321 tgacaaagat gcattactag tacgacaaat tcatgtccaa gaacgccaca tagtacaaat
    13381 gaaaaaaggg tggctaaaga tgtcacacgt aaagtcatca aagggagtaa gacaaagact
    13441 acagttagag aaaagaattg tgcaaatgga ggaaaagttg ccggagatgc caccagaggc
    13501 aaagaggatc aagataaatc tacccgagcc gaattgggtg ggaagtgtac ccttgatcgc
    13561 ggaaaaaatt agcaagtctt atggaacaaa aaatgtctta agtgaaatcg attttgatat
    13621 taaacgcgga gaaaggatcg ctttaatggg gccaaatgga gtaggtaaaa gtactttgat
    13681 aaaaattttg atgggaacaa tagagccaga tgaaggagct atggtcaaag atgaaaaact
    13741 agatattggt tattactctc aagaatttga gatatttgac atggaaaaga atttgatgga
    13801 gacgatgaag gacttttgta gtttccctga gcaattaatc aggggtcact tgggaaggtt
    13861 tttgttttct ggagacaaag tgtttcaaaa agtaaaaagc ttgtcaggag gagagaaaac
    13921 tagattggca ataggaacat tgctagtaca gaaacataat cttttgatat tggacgaacc
    13981 gacgacatat cttgatgtat tatcacaaag actaatacta gaagcactca aggcatataa
    14041 aggggcgatg ttgatcgtat ctcatactcc agagtttatc gaaggattga aaccagagag
    14101 aaaattcttg ttgccggaaa agaagtggac tgtgacataa taaattaatt aaaaattaaa
    14161 aataaaaata tggatcatgg atgtaaatgt aattgttttg tgtgtaaggc tggtaagttt
    14221 ttaggaattt tggatgattg tagtaataaa aaagattgct gtaagacccc agtaaaaaaa
    14281 gtggtgaaga aaaaagctgt ggctaagaaa gtagtagcta aaaagaaagt ggtgaaaaag
    14341 aaaaaataaa gagtataagt taattgatgt tgaaaattcg tcagtttcta aaaaaggttg
    14401 attggaaaat ggtcggggta attttggtag caataatact gaggctggca ttaactccga
    14461 caatatttca tggagatatt attgctcagg ctggctgggg aaaatggata tttgataatg
    14521 gaataaaagg attttacgaa aataatgtat ggatatacgt ttggccaaac cagccgccat
    14581 tgataagttt gctgtattca tgggggtttg agatatatgc ccagataaat acaggactaa
    14641 tattgttggg agattttatt gctagaaaca ggctaggggc cagctacatg ccatggcttt
    14701 atacatttat agtctggtgg ggcaccgaga tgttttccga cacgcctttt aaagttgggc
    14761 agttaatttc gatgaagtta tggccaattg cggcagactt cgtattggca tacattattt
    14821 ttgtcatagt aaaaaaagcg accgatgtaa aaaaagccaa attggtaacg gtggtatatt
    14881 tgctgtcccc ttttgtgtgg tacgaaagtg ggatgtgggg acaacatgat caattggggt
    14941 tgatattttt gttgttggct tttatggcaa tggtgtcaga gaggtatagt ttctggtcgc
    15001 cactattgat gatgactagt atattactaa aaccaacagc cctgtttttt gggccgctac
    15061 ttttctgggt gggaatgaaa aacaagacag tgtttttgaa aatgatggcg gggtcagtac
    15121 tggcattagc tgagtattat tggataacaa gaatattttc agattacagc ttcgtaagct
    15181 ttagcgtaag tttgaagaat cagatgtttg tgaagggaga gataatgact tgggcaaata
    15241 cattcaattt atggcgggtg attactggat acattactga ttataaaaat atattcctag
    15301 gattaactct aaaaacttgg gggtacgtaa tgtttgtacc gatatatttg ttgggtctaa
    15361 aaattagcca aaagagagat tggtggagtg tgctaaaggc catgtttgtg gttggttttg
    15421 gagggtggat gacgatggtg acaatgcatg atagatatct atttccggcg gtggtgctgg
    15481 gattaatatt gtctagcaaa gaaacaagac tgtggaaata ctgggtggtg atgagtgtta
    15541 tcttttggat aaatatgtac aacggctggt ggacaccgaa gtttatggat tggctgagat
    15601 atattttgac ttttgataac gaaatggacg gaatggtgcc aaaaatattg tcgatggtga
    15661 atgtgtggct gatggtgagg atgtttggga ttgtgaggaa agataaaaag tcattatagc
    15721 ttgtaatcgg agataaactc atagctttcg tagggtttgg gatgatttag gatacaaagg
    15781 tcgagctcag gccagcgacg aggtagggat tttatttcat caacagataa ccaagacaaa
    15841 ttagttaaca atttagtaga ttttttctga gcatcggtga gcacaatttg aaagattgca
    15901 aaaccctcgt caaacaggac ttcgttagtg ctcgttttaa tcattttgct gtgaagagaa
    15961 acaaaattta aactcgaggg gtcgatatca ataccaatat tctttctgac aatgcgtgtc
    16021 atagcctcgt ccatactttc tccaaactga ggccaaccgg aaggtaaatt gaagaaattt
    16081 tccttggcaa cagggtgact tttgattaaa taattatctt gctcgtcttt aataagaaga
    16141 ccaacaaaat atcttttatc accaacatcc gccagggtat ctaactcata actgtaaacc
    16201 tctttaagac cagaagagtt aatagaatat agatctttat ctttaattaa aaaaccatca
    16261 gcaataagtt tttttaaatg aaagacgata ttgtcttcat aatcgtaatc gcgagttaga
    16321 gtggaatatc gttgattgtt ctgttgagat agacgcttca gaagaatctt ttggacttga
    16381 tgcatatagt gtagtttact actctagaac tatttttaat aagcggtata ttaccgatat
    16441 gttcaaaaaa atgtttgcca actgtctact agttaaagat tttgaaaaat catttgattt
    16501 ttacaaaaat aaattagaac tagaagtgaa tgtatgtgag ggaggttttg ctaattttaa
    16561 aattgaaaat attgaattgg caattttgga tagaaaaaat gtggtagagc ttggagtaga
    16621 gaaatattta aataatggtg gaagtgtatt tttgtgtttt caggttgaag atattgagaa
    16681 aacatataaa aagttactga gtcatggagt tgagtttata actgccccaa aagtaacttc
    16741 ttggggacaa aatgtagctt atttcctcga tccagataag aatatctggg agatttcaaa
    16801 gatatgatca aagcaatagt ttttgactat gatgagacgc tgacaagaac tattgacggt
    16861 aaagccaggg cgtactcgga ctttgcgatg gctgagtatg gaaaagtact aactacagaa
    16921 gatgttaaaa aagcctttgg aataaagtac gaagaattta ttaaagtatt gtttggagat
    16981 atcgaaacag tagaaaagat tattggaaaa tatcaaaaat ttagtgccaa ctatcctcca
    17041 ataccgtacg agggtgctat agaaacagtt aataaattat ttaaaaaata tctaatagga
    17101 attgtctcgg ggataaggag aaaagattta aataacgatc tagagcaatt aggttttgac
    17161 caaaaaatgt tttttcatat tcagtgtgga gatgaaacaa agatattgaa acctaatccg
    17221 atagtttttg agccgttaaa gaataaatta aaactgtgtc aaattaaacc agaagaagtg
    17281 gtgtacgttg gagatagtct gggtgacatg ttggcggcaa aaggtgctgg atttaaattc
    17341 atcggtatag ctgggcacac tagatcggca gaagagttca gagaaaatgg agcagaaata
    17401 attgaatcac ttagtgagct agaagaaatt tgacttgatt tgtattattt taatctattg
    17461 tcttcggttt ttattcgggt tattatatgg ttatgaagac aatattaatt atgacaggca
    17521 tgatactagt ggatatgctc ttgttcgtcg acatactggc aactgcagag gcagtaggaa
    17581 taagatagaa taaggtgatg aaacgactgg ggttgataat attactagga ttgttgtcgg
    17641 taagaggtgc gtgggcagcg gataaatcgt attcgattga taaagtagac ataggagcca
    17701 cagtaaacat agatgggtcc atgtcggtag aagagaggcg agaatatgat tttgatggaa
    17761 gctatacttt tgcttatctt tatataaatc gacaaggaga aggtagggaa aaatacaact
    17821 tatcagattt taaaatttgt gatgagataa cttgttacaa aagggtaaac agtcaagata
    17881 atgtagctaa caatttctat atattggagg aaccgaatcg ttattatgtt aaatggtttt
    17941 atagagccag caatcagaca aagaaattta aattaagcta caaagttgat aatgcaataa
    18001 cactacataa agatgtggcg gagttatact ggaaatttgt aggtgatgac tgggagattt
    18061 cacaaaaggg agtgtcggta aaagttaatt tacctagcgg gataaaagac aatgaaattc
    18121 aggcttgggg gcatggacct ttgacaggag tagtggcaat accaacaaaa gagctggtaa
    18181 cttttacaac aacaagtcta ggctcgggga ctttttttga agggagaata ctactaccaa
    18241 aaaatgtgtt taaaaaagga gtggtgggga aattaacggc agaagagata agaggacaag
    18301 aacaaaaata cattgacgac acaattcaac aaattaaaaa tcggtcgatg gtgggtaatg
    18361 ggctagggtt aatatctata ctcttactga tagtagcgac aaaaaagttt ttgggagaaa
    18421 taaagaaatt ttggagatat cagaaagatg aaaagctgcc aaaagtaaac ttgtcgggaa
    18481 gaatttggga accaccaagt gacatcgacc cggctcagat agagcaactg atatcgacca
    18541 ataaagaatt aactccaaag gcattcacgg caacaatatt gtcgctagtt caagacagat
    18601 tttataaact gacaagaagc gatcaaaaag aaggactcat cttcaaaaga tataactact
    18661 atttagaaaa aattgataat aagaagattc cgagtggaat acaggaaaag gtgatgaatt
    18721 tcttggataa aatggatagt cagaaaatta agctggctga tatggcagga tggtgccggt
    18781 cgcatcagac aacgagttat aattttttta agtcgttaaa agaggtggct tttaaggaaa
    18841 atcttaacga gggatatttt gacaaagagg caaataaaat aaataaaagc tacggtggaa
    18901 tagtactgga ggtgttaata ataatactgg tgtttgggat gacgatagta tctgccgaga
    18961 tgaaggcaaa tcccctattt ttaatgattc aagggttagg gatagtgatg gttctgatat
    19021 tcatcgtgat gatggcagtt atgaaggttc atggagaaaa aagaacagaa aagggtcgag
    19081 aggaaacagc caaatggcta gcctttaaaa agcacatgga agaatataaa aaaactgtag
    19141 aatatccgat tgactcgata gttttgtggg aaaaatactt ggtgtacggg acagccctag
    19201 gcatatcggc aaaagcattg gcagaattac caattaaatt cagtcaagca gatgaagggg
    19261 tcggagtgat gtattgggga ggaacgaatt ttaatattgg ggacttttct tctttaaatt
    19321 cagcaattgc ttcggtggca agtacagcgt cggcgagcta tggagcttca ggatcgggta
    19381 gtagtggtgg attttctggt ggaggtggag gaggaggcgg tggtggcggt ggtggagctg
    19441 gttgagataa agtgacgaac ataagggatt ttagacaaga gccaaacaga agaaaaagat
    19501 aatagaacag ctaagatgaa aacagacaaa aaccaaggcc aatagctagt aatggaaata
    19561 ttttttaaaa tttccaacca aaaagggtgg ataagataaa caccaaaagt taaattagaa
    19621 agtttcaata aaacatgaga gtgtttttgt aaaatattgt ttgataagtg agataaaaaa
    19681 taaaagatta gcaaagcgat accggcaaca ggtagggaaa gattactgac gagaatagta
    19741 tcttgatcgt gggtttcaaa aataaaaact agtttacgag tgacaaaaat agacagaaga
    19801 aagatgaaaa caatcaacga actaattatt ttaaagtgtt ttatagtagg caaattacca
    19861 actaagtacc cagcaagata ataaccaaga taagggatga accagatgaa gatgttgttg
    19921 agccgaggaa aaccaagaaa actataacta taatgataga aagctgaaag taatataaga
    19981 attgggacaa ctatttttaa gttagtttta gaaagataat tggtcaaaaa tggcgttagt
    20041 agataaagac cgaccataat atttagaaaa taaagatgag aagctgtgcc agaagcaaaa
    20101 agttgggaga agaactcaac aactgatcca gcactagaat gatggttaaa aagataataa
    20161 aaaatgttcc aaaaaattaa gggaatagcg agtttgtgaa aacgcttttt ccagaaatct
    20221 gtagcattag ttttggcagc agaaccaatt aataaatacc cgctaaccat gacaaaaagg
    20281 ggtgtccccc agataactaa ccaaccaaga gaagcggaaa cgatccagtt tttgtaacca
    20341 agctggtcaa ggtcaaaata acgaagggaa acagtatgaa ttaaaataat agccaaggca
    20401 gaagctaggc ggataaggtc taaagatagt tgacgatctt gattcttgat actgaagtat
    20461 aataacaata acaatgaaaa agttaataat gtttgttttt ggactgatag taatgggggc
    20521 gacgaaaaat ggagaggtct gggccaatga aaaatatacc acaataatta atccggt
//
LOCUS       DDWP01000086            4326 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_48331, whole genome shotgun sequence.
ACCESSION   DDWP01000086 DDWP01000000
VERSION     DDWP01000086.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_48331"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1170 a         1014 c          793 g         1349 t
ORIGIN      
        1 gcttgcgctc ggaaccgagg aggggagaga agaaaatctg ttacatgagt ccgaggtagt
       61 atccgccggc cacgatctta gcgaatactg ccaacgtcat cccgtctttg gattgattga
      121 cgtactcgag atactcggcg atggggcgaa ctcggaacga gctggtcact tcttgtttgt
      181 cttcttgttc actttcacca aagtcaggga tgctgtctac atcgacgatg gcaaagaaga
      241 tgcccgggca gttgttcgtt aaaccggcgt caacaaagta ttcgcccaag gggacaactt
      301 tggatacttt tactgtaccc ttgagttctt ctttgagctc acgcactgcc gtttccagca
      361 agccttcgcc aatttcaccg aatccaccta ccaggtcacc gactgtttca ccacccacgg
      421 cgaatttttg agaagtgatg tccacaaagc ccaccacctt gccgccgttg gcgacgaaaa
      481 cctgaccttt tgcgccagat gcccggccat gtctccacga ccagtacata tagctttgga
      541 gcttatcacc atcgtgctga ttgatgactt cgaagtttac gagtgcgccg aacttgccaa
      601 catcagtcac gaaacggata atccgtttgt gctgcaggcc gccgacgact gtcgaacggt
      661 tgatcaggtc ttcaatgaac ccatcccatt tcttgtcagg ggtatgaatg attgccaact
      721 ggatttccag gtcggtgttc ttccaccagt ccagtccttc cagccgcgcc aacagtgcca
      781 tacgattttc gcttaacggt tgcattttgt atctccttgg tttgtttgat ttgaaaatca
      841 aaaaaactcc cacgcccacg gcgaggagta caaggatccc aaaacagcag gatccaaaaa
      901 acaacatcat aaaaaacatt tcatccattt ccaagctcct ttcgattttc ctagtcaatt
      961 tttctccttt ctccggccta ggtaccagaa aataccccca aaactaatta ggaatatttt
     1021 ctgataccaa gacgcaaaaa ttaccgcaac ggcagtttga aaaaagttta aaaatttttt
     1081 ggaaaaatct tatccgactc acgtactatc tctccgattt gcatcgggag aatcacataa
     1141 cagatcttct tgttaaagtc cgagcttgtg gctcgttttc aatacatcga ctataaaagt
     1201 ctgaggggat tatatccaat aatattcccg atgtcaatac tataagacat acacctctca
     1261 gttaacgctt gaccattgac ctatttattt ctacaatcag taacctatga aaatacttat
     1321 aactggaggt ttaggtttta ttggttctaa ttttattcgt tattggctca agagacaccc
     1381 cgatgatcgg atcatcaatc tcgacaagtt aacttacgct gctaatccgg ccaatctgtc
     1441 tgactttgat tcaaatccaa attattccct agttaaaggt gatattctcg ataccaacct
     1501 tcttgatcaa atagtccccg gagttgaact aatcgtccac ttcgccgccg aatcccatgt
     1561 tgatcgatct attgacgacc cacttctttt tgtcaggaca aatgtcttag gtacctatac
     1621 attattatcg gctgccaaaa aacacggtaa tattcgtttt caccatattt ccactgacga
     1681 ggttttcggt tctatcgagt ttggttcaga tgaacaattt tccgaagaca ccccttatga
     1741 tcctagctct ccctactcct cctcaaaagc ctcatccgat cactttgtcc gctcttttag
     1801 taggacattt ggtcttcctg tcactatctc caattgttcc aataactatg gtcccttcca
     1861 acaccctgag aaaatggttc ctcgcatgat tactaattta ttagatggta agaaagttcc
     1921 cgtttacggt aaaggtgaaa acttccgtga ttggctttat gtcgacgatc attgctctgc
     1981 tatcgaagcc atcattcaaa aaggtaaact aggggagacc tattgtattg gtggtcttaa
     2041 agagacttct cgcaacattg acctagttca caaaaccctt aagctcatgg atcgtcccga
     2101 atctgagctt gagtttgtca ctgaccgtcc agctcacgac aattatgccg ttaattggga
     2161 caaaatcagt cgtgaattag gttggtctcc ttcagaaact ctcgactcgg gtctacaaaa
     2221 gaccattgag tggtactctg ccaacgaatc ctggtggcgc aatgccaagg ccgaagccga
     2281 agccttctat atcaagctta ataattacaa aaatgttaaa tagtatggct gatgttgttt
     2341 acaatatgga cattcacgaa tcctcagtat ttaagggggt ttggtttatt caaccaaaag
     2401 tttttggtga tcagcgtggt tattttgttg aaacttattc tcagcgtgaa cttgagcatg
     2461 ccggtgcccc tgtcataaat tgggttcaag ataacgcctc tctttcttcc aaaaatgttc
     2521 ttcgcggtct tcattttcaa ctaccacctt ttgctcaagc caaacttgtt ggtgtcgcta
     2581 tgggtgaagt tcttgatgtt attgtcgaca ttcgccccaa ttctcccacc tttggtcttc
     2641 acgagtcagt cactctttcc accgagaagc agaataaagt cttagttccc cacggttttg
     2701 ctcatggtta tgtggttacc tctgaatccg caatctttac ctacaaatgt gacaattatt
     2761 atgcccccga tcaggcaaaa gttatccgtt gggatagttt agacatagat tggggtattg
     2821 tttctcccat cttggctcaa aaagatgccg aagcttcttc atttactgac atgtctgaat
     2881 ttttaaaatc tattaattgg taaataaaat atgaaaataa ttggcactgg tctttctggg
     2941 ttggttggtt cgcgtattgt tgagttaaat ccaggtatag aatttgtaga aatttctctg
     3001 gaaaagggaa tcgatctctt agatttgtca tctctagaac aggtcttcat caataatcct
     3061 gatgctcccg ctgttttgca tttagccgct ttcactgata ctaatgttgc ctggagtcaa
     3121 aatcaagata agtcaggttc ttgttatcag gtcaacgtcg tcggtactca aaacatcgtc
     3181 gacctttgta aaaaatatga caaatattta attcatattt ccaccgattt tgtttttgat
     3241 ggtaccaagg atggaaaata taccgaagag gattctccta atcctatcga atggtatggt
     3301 gagacaaaat actttgctga aaaagttatc ctagactcag gtattaaatc ttctatcctt
     3361 cgtattactt ttccctacag gtcaaaccat gattccaaga aagatattgt tagaaagatt
     3421 atcgacggtt tcaaggctgg taaactttac cctcaatgga ctgaccactt tactacccca
     3481 acttttatcg acgatatcgc ttcagctgtt aagtatttta tagaacatca accgtcaggt
     3541 attttccacc ttgctggatc ttcatctcag tctccctatg agatgtgtct tttgatcgcc
     3601 gacgtttttg gttttgatag aaacatcgtt gttcaaagta cccttgctga ttatgtcaaa
     3661 tcacttccag aaggttctcg cccctggcaa cataatctct caatggacaa caccaagatt
     3721 acttcacttg gtatcaaaat gaaaactctc cgtgaaggct tagaagagat gaaaaagcag
     3781 ctagagctct aatttgttgc caagttcttt ccatgaccac actcgttcaa tccctgccgg
     3841 gacctcttca tcagcgttat acggccaaga aaataaatat aaatttggta ctagtccaat
     3901 taaaggtatt agctttttca gactgtcatc aataaagtac tcaaatcctt ctagtgcctg
     3961 ggttttttct tgtctattag taccgaccac ctcaatgcct tttaatccat taatttcaca
     4021 ccatcttttt gccaaatcta cccctgtttg tgtcctcgat gtaactatag ttatatcaaa
     4081 actttgtctt aagacttcga tcgtttcaac tgccccaatt accggttcag tttccattac
     4141 tatttctgat tcatacatca ataccgctaa ctcctgatat tgatcattag ttataaatcc
     4201 gtttttcatc acagcttcgt atccagtcag gtccagaggt atttttactc caaacttctc
     4261 ttggacaatt tgctgtttaa ccgctctcga atcagatatt actccatcaa agtcaattcc
     4321 aaattt
//
LOCUS       DDWP01000087            3509 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_70927, whole genome shotgun sequence.
ACCESSION   DDWP01000087 DDWP01000000
VERSION     DDWP01000087.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_70927"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1032 a          645 c          587 g         1245 t
ORIGIN      
        1 ttatatcctc caaagtagta tctgcattta tctccaacat taacgcaccg cgttctccaa
       61 ctccggtgac tatcgacaca tttcccggaa tcatcattga ttcaatgttg ttaaacatca
      121 agtatctttt aactgattcc agccaattta ttggcaatcg atattttgag actagatttt
      181 taaggtctcg atgaaaatca ttaattggga ctctgttgtt aaattctttg ttagcctttt
      241 ccttacctaa ccaatctttt aacttgatta acatattttc tacttccgcc tcagccttcc
      301 tgttgccatc tataaattcg tccgatgctt cacaaatcca attgtgccat ttttgatttt
      361 ctacatcgga tacaaaacca cattcagaaa tattccactt ttttcgtagt gatttgattt
      421 cttcttgaaa ttttggatcg gaaattagta ttttggcctt ttggtgtagg cggtttcgag
      481 ttttagtaat atgactgtct ttcatgttct agcaggatta tatcgattct acgctattgt
      541 aggtaactat gttgacaagt acataactaa ctgcacctca aaaatatttg aacatatcac
      601 aaagtagttt aattaaagaa actgacaatt tattgaaaat ttaataaaaa cgtgacaata
      661 acattattat gtcgtatgtt gtataattaa ttagctatgt cgtacttgcg aatatctcaa
      721 gttgcaaaaa tacttaaagt aaatactcgg acccttatga gatgggataa tgaaggtaaa
      781 tttgtcgccc acaaagaacc actatcaaag atacggtttt atgacgaaat tgacatacta
      841 aaccacgagg tctggttcga acttaggaga aaacacaaag cgcatcttaa gaagctcgat
      901 tcaataagaa aagaagtgga taaatttgtt gtaactcagc cattagaatt cggcgccaac
      961 ccaaagtttc ataaactcga ggatatgaaa aaggcatatg atgcactaag agattgggaa
     1021 aaagaagaaa aaaagatcac gagcgagtat ggtaagttac cgagtggttt taaagcaaaa
     1081 gttgaccctg aaacgtagat agtgaaatgg gagttctagc tctctaaact ttttttttga
     1141 tttttaatcc tctcaagaag ctcatcataa agaacaattt cgatattttt gaactgattt
     1201 tttaatattc taaagtcttc ttttttcgaa gagttgtcta gttgggctga gtttcccata
     1261 attacgaatg cccttggtcg tatgacatct aaatcaatgt ctttctcacg tttaacatca
     1321 gcctttaagt catttccttt tcgctctgaa tgataaagat atttttcggc ttggacgata
     1381 gcttctattg cttgtttgtc ccacgagtaa tttcccctac tatctttttt ggcgattaat
     1441 agcttagttt ctggcttttt gatttcgaaa agatcaagat acccctgggt gtccaccaaa
     1501 ccaaagtcaa cttttctggt tgttccaaga attacattaa gctgtgcgat tgaatgaatg
     1561 tatttcgaat ctattaaaaa tagattctct tctaaaaact ttccccattc agattctgac
     1621 gtcttttttg atgacagttt tttgtcaaat ctatcgatta catcactaaa tgtcaccgag
     1681 tcaattttta gttttgtatt tttaaataaa tcgctttggg taatttgtaa tcgatggctc
     1741 gatttcatta gggttgagac aatatccgtt actctaccga tatcgtgttt atcaaaacta
     1801 tcgactcctt gggcagacag tgtctgaatt gcaagctttg tatcagccgc ggcactatta
     1861 atctttttag ctttcaccac ttttggaaat tgtgtattaa ataatttgtt aacaatctgg
     1921 gcgcgtcttt ggcgatggta gtagtccatt gtgccgagtt catcggataa cccctccaga
     1981 caggaatatg ttagatgtaa atatttattc ttcccttttt tagtaataga acacgctccg
     2041 gtcttggaga tgtcaattga ttttatgttt ttaccactga aggttcgatg aatatagtca
     2101 aatatacggt tcttacaata tccgtttttc ttaaatattt cgggtaaaga tgagaatcca
     2161 tcaatggtaa gtttagagaa gaagtagtat tcagacttaa ttgggtagaa ctcaataaaa
     2221 agcttgtttt tgttgatttt gtaaacaagt attctttctg gagatttttt atcatcaata
     2281 acatatcccg aaataatttc gctccctcta atttttttga cctcttctat tgtttttaat
     2341 gccatatatt ttacttttga ttaataaagg tattaaaatc catagttttc tcctgtttat
     2401 tgacaaacca gaaatgatct tcagttatcc caaaaatgta acaaaattca gcccatattt
     2461 tgtaagcaat tatttcggga gtttggtttt ctttctttat aaagacatct ctatcgaccg
     2521 cggggcccag aaagtctaat cgaccagcag acgaaatcca gtttatatct agaactagtt
     2581 cgattatctt tctctcaggt ttaaatatat ggattttata aatgatccca gacggttttt
     2641 tagctaggtg ttccgatatt ttatttgtaa agttgacaaa gttggatata agttcagccg
     2701 ccgctaaacc attaaattta aatgattcgt ttgggtcttc gttttcaata ccatgtccgt
     2761 aaaaatcctc actagcggcg aatataattt gaccatctct aaatatccta ataatctttc
     2821 tgtcaccgtt ggtgatttcc aaatattcac cagcaactgg ttttgtgcgt ccgagtgtcc
     2881 tcaaatccca ccccatattt ctaaaactaa cagggttctc gatgtattta gttaattccg
     2941 actcaccgcc agcgaataaa tccttaactt catttggctg aggtctaaat gagctggata
     3001 tggcaaaagt agtgaagttt ttaaaatcgg aaaaattctt aatgtcgttt actctaccta
     3061 tttcttcggg tgtgaatgtt tgatgaactt cgttggcatt tttttctaaa agatatgtcc
     3121 ttatcgcttc tctaatcaac tcacttctat tcctaatctc aacttcagct tgcttatcaa
     3181 ctgcagctaa caattcctca gataaagtta tgtttataat ttggtttttt ctcattaact
     3241 aattgtacac acatgtgtgt gtattttcaa gtattgtggt taccgaattg agacactcct
     3301 tttttatact gtctcatttg tcttatttag cttcaaatct ttgatagaat gagacagtgg
     3361 ggaagagggt gttataggga cagaaaggtt ccagacccta atgctccgca taaaaaacaa
     3421 tcccgatgaa tcgggcaata ggaggagaac tggtgagctt gtcgaaccac tccctctcct
     3481 tccgtacccc ccaaaacatt gttttttct
//
LOCUS       DDWP01000088           50347 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_4448, whole genome shotgun sequence.
ACCESSION   DDWP01000088 DDWP01000000
VERSION     DDWP01000088.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 50347)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 50347)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..50347
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_4448"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    4281..4293
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18798..18813
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        16489 a         8921 c        10552 g        14347 t
ORIGIN      
        1 acagattcgc aagattctga aacatcggtt ggagattggt agattttaac tttcatggtg
       61 tcagagttgg aggaaaaact gacaggtatt gtgaaggttt tgtcgttttc tggtaaacca
      121 ctagggttat cagcacgaaa gactaaatag ccattagaac tattgactct gaagtttaaa
      181 ttgcaagtaa actcgatcat ctcttcttgt gtccaagatc caggaatagt ttcagagcaa
      241 ttggcactac tgatattttg atagtcacta ttcaaaagct caacagagaa agtgccctca
      301 aacatccaac tactttggac cttcccaact acttcaaatg gactggaaag cggagaatct
      361 atagagacag aaattggagt ggggacgggg ataagggttt tttggctaac aggagaaagg
      421 ataatgataa ttgaagacat aaagataaca gctaaggcag cgggccagag atagatcttt
      481 tttagtttca tctgtagtaa ggatacaacg tcattaataa aatgacaaaa gcgatggcaa
      541 atagaaaagc cgagaggata aaatatgttt cttcggtttt tcttaccatt gaggcatata
      601 aacttttaca ttagattcgt cgacaactaa ccggttgact ttacccgaaa ggatcatgag
      661 ggcagcttta cgacaaagcc cttcaatatt gcgcttgaga gtacgaacac cagcatcaaa
      721 accaaggggg cggacaacat taagccaaac attatcggta ataaccaatt cttcagcaga
      781 gaggcctgcg tctttgagag cccggggaag aagataatca cgaccaatgc caatcttttc
      841 ttcatcattg tatgaaggca tttggatagg ttcaagacga tccatgacgg cagtagcaat
      901 acgagtagta ttgttacagg tggctacaaa taatgattgg gaaagatcaa aaggatagtc
      961 gatataatga tcggagaaac gatcattttg ttctgggtcg agaagttcga ccagaacacc
     1021 cataatggtg ttaagagcat catcggcgac acgatcgatt tcgtccaaaa gtatgactgg
     1081 gtttttggtt ttggcagtag ctaaacctcg aatgatagca cctggctcgg cttcaggatg
     1141 aagacgtgat tgacctctta aatataatgg gtcacccaat cctccaaagg ggatacgaat
     1201 tagctctcga ccaagaactg aagagattga cttggccata gtagttttac cggtaccaac
     1261 taagccaacg aacaaaagaa tcggggcgcg aagtttatga tctggattac ggtttttttg
     1321 aagagcgagg acagagatat attcaagaat acgttctttg accatatcga gaccataatg
     1381 gccttcgtta agtttttcgc gggcaaaatc tagatcaagg atgtcttggc tttctttatc
     1441 ccaaggtaga gcaatacacc aatcgacgta tttattaatt gactgataat attgccaaaa
     1501 agtgtcttct gaacgagcgt taatacgcag ttgagaaaat aattcatcaa gtttttcttg
     1561 aagaccatcg ggtaacttgg aagaatggct tttttcaatt agcctatcaa tggcactttc
     1621 gggagttaac gttttctcat ccatagaaaa agtatacacg aaaaaaatac tccggcggac
     1681 accggagtat taaaatttaa aatatttttc tatcttcttg gagtggaaaa gttacgaaag
     1741 ccgccttgtc ggggacgatc aaactgtggt cgctcacgat ttggcatagc attcggatct
     1801 cctggatgag cagccttgaa ttctggtgca cttaatttta tttgatgatt gtcattaaag
     1861 ccagaaatag tgacgcttag tttatcccca agcttgataa tggcactagg atcatttaag
     1921 aatcccccgc ttaattcaga aacatgaagt aaagcttcac gaccgggaag gaattcgaca
     1981 aaagcaccat agttttcaac acgagtaacc acgccctcat aaacttcacc gatctggact
     2041 tctttgatca tggcttcaat ctcgacagca caagcatcaa ttttatcctg atcaatagca
     2101 gatatagaag ctcgaccatc atcgtcgatg tcgatttgaa ctccatattt ttccattaag
     2161 cccttgatgg tcttaccacc agaaccgata acttcaccta ttttatcttc aggcaaaatg
     2221 actaatttta tttttggagc ataagctgat agctgaggtc taggagcagc gagaactgct
     2281 tccatctggt cgaggatatg aagacgagct ttggtagagg cagcgaaagt gtcttcaatc
     2341 atttgcatgg ttaagcccat acatttgata tcgagctgga tagcagtaat accagtacga
     2401 gtaccagtaa tcttgaaatc catatcaccc caatgatctt caatgccagc aatgtcggtt
     2461 aaaagtttgt atttaccagt attatcggaa atcatgccga cggagatacc agcaactttg
     2521 tctttgattg ggacaccagc atccatcaat gacatggtgg aaccgcaggt agaagccata
     2581 gaagaagaac cattttggga aagtacttca gaggttaaga caatggtata tggaaattct
     2641 tcaatagaag gaataacagc aagcaaggct ttttcagcta aagcaccatg gccaacttct
     2701 cggcgtccag gacgaccaac tcggccagtt tgaccggtgg aaaaaggagc ggcagaataa
     2761 tagtgaatat atcttttggt aaattcacca gtactgtctt ctaaatattg ttgttcagcg
     2821 agcgaaccga gagtggtaac tgtgacagcc tgagttaaac cacgttcaaa aatagcggaa
     2881 ccatgggtac aaggaagaac tcctgcttca atattaagag gtctaacttc gtcaaaagga
     2941 cgcttgtcgt agcgtgaccc acttaagtaa agttcacgga aatgcttgcg agcaacagta
     3001 tcgacggctt ccattaaggt tgattctttt acttctcctg attcgatctg tggtttgtaa
     3061 gtttctttta tcttgtcttt aagaatttct tcaccagcaa tgtgagcacc atcaaaacca
     3121 gtagacaaga aagttttcaa ttcttcagta agatatccct caacttctgc caagagtgtt
     3181 tctgaaggta attgactgac aaattcaatt tttgttttac cattttcttt aatgaaagtc
     3241 gaaagttgtt cgttaataga aacaccggta tcattggcta atttcatagc cgacaacatt
     3301 atatcattat caacaatctt ggcatcggcc tcaatcatgt tgataccttg agaatcgtga
     3361 cagattaaaa gatcaaagtc agctttgttt tgttgctcga ggctggggaa taaagtaaat
     3421 tcagaactaa caccaccaat acggactata gaaactggag cattgaaagg aatttgagaa
     3481 attccaatag ctgcagaagc agccaagaaa gctgggataa ccacatcgtt ctgtttgtcg
     3541 ttacttaata cagtaaccat taattgaact tcgtttttga gaccagcagg aaatagaggt
     3601 cggacggcac gatcgataat acgaccaaac aaaactgagt tgtcagattg attgccatcg
     3661 cgcttaatcc atttgccacc tttaatgagt ccaccagcat aaagcttgtc gacgaattca
     3721 acagaaagtg gaaaataatc cttggtggta tctaaacgac caagaacgac agtagctaaa
     3781 acaactgtgt taccagattg aacaacaaca gatccagaag cttgtggagc taactgacca
     3841 gtagagaggg tgaatttttt gtcaccaaca acaatatcga aagatttctt atccatgtag
     3901 attgattaaa ataatatgac taattgtacc ttacttcttg agcccgaggt ctttgatgat
     3961 agcagtgtat ctttcgacgt tgtgactttg aagatagcga agaagacggc gacgtttggc
     4021 gattttgctt aaaaggccgc gcttagctgg agaatcgtgt ttgttctcgt ttaaatgacc
     4081 ttgaagtttc tctaattctt tggaaagaat agccacttgg atttctggag aaccagtatc
     4141 acccttagct tgggaatgct tgtcaataat aagctttttc tcttcggtag ttaatgccat
     4201 ggtattaaat agtgcttaag tcggtaaaaa ataatatttg ggaatggcta cccaaaaacc
     4261 gagataagta cagggggatt nnnnnnnnnn nnnggcatgc cggcagactt ttcaacattc
     4321 tcaataggca ttggctctgg agaggtagca acaccgtcag cttctggctg ggtagctttc
     4381 aaacagacag caccagcttc aaaagtctct ggtgagacgt taactgagaa agactgagta
     4441 gcgacacgaa gaccgattaa aagattctta ccggaatcga tatcgagttc taagccgatc
     4501 atgggtaaca agaaggtaaa aatctcagaa aaaggagcat caggatcaac taaagtagcc
     4561 ttagcccaag ccttagtaac atttgtaggt gagataaaga tccaattacc tttgttagta
     4621 atagaagtgt tatttcctag tgataattca tctccaagca tgaaacagac atcagcatta
     4681 ccaccatcat tgtcaatatt aatcttgttg ctctcaactt ttggagcacc agacttagtc
     4741 ttaacaataa gattaagacg accaccgtcg tcattgtagg agacttgttc gatttggtct
     4801 aatgggtagt cgagagagac agtcaaatca cttgaggaaa tgacgtcagt taggagctca
     4861 agaccactta atttagaata attttggtca gttttagtag gacaatagac cttagcttct
     4921 ttaccactct ttttaagagc gatggccaga ccaagagcag ccccaatact gtcaacattc
     4981 atttgtggga caacaatcaa ggcagtttta gcggtactta tcaaattttt gatgtcagaa
     5041 acgttgtagt tcttcatatt ttttccctct ggttgggaat taataaaagt agcatttaga
     5101 cgactcattt gtcagtctta taggatatta taacatcgtt tattttaaag tcaatataag
     5161 gcttgaaagt cataccacac tcagtggtag ccttaacttt ctcgagtata tctttgccaa
     5221 catgaatacc ttcaactttg gtgtctttaa tgattttatc gtctctttta agatgaatag
     5281 gatcaccttt gttgatttca cctttataaa cttgaattcc ggcgatacgg actttttcaa
     5341 tcttaaactc agcgatgatc ttaccttcac ccaggattgt ttcttcaata gtgggatcaa
     5401 gcattttcag gacctgagtc tggatatttt caattaattc gtaaattatt tttgactcta
     5461 ttatgggtac tttttcatta tcagccatgt tcttgataaa gcgaggtacc gacacattga
     5521 aggcaaagat ttgggcctta gcagcagagg ctacaaagac atcatgatcg ttgactggac
     5581 caacaccagt gtagataagt tggacatcat cagaaaagct attttttaaa gcttcaagag
     5641 taccttgaac atcggcttta actacgatag gtaagcgaat aataccgtta aagtcttttt
     5701 tttcggttag cggaataatt gcctgagcat ctaccgaatg atcgagcaca agagcaccgg
     5761 ctgcaggaac tttagcaaaa ccaagtattt ctacaggggt tgagggagga gcaatggata
     5821 tattttcacc ggtagagctg atcatggcct taactttgca aaaaatgtca tcagcataaa
     5881 taatgtcacc tatctttaat gtgcccttat ttataatgac gttggcaaca ggaccacgac
     5941 tattgtctaa acgagattcg ataatagcag cctcaagagg agcatttggc tcgtctttta
     6001 attccataac ttcagcgttt agtaagataa cttcgagtaa ttcgtcaata cccacaccag
     6061 tcttggcaga tacaggaaca attgttactt gaccgccata ttcttcggga gtcaggccgg
     6121 cttcaactaa ttgcccttta actttttctg gggaagtacc ttcaagatcg atcttattca
     6181 tggcaacaat tacaggcaag gacgatttgt gaatctcgga gatacattct ttagtctgag
     6241 tcatgacacc atcaactgca gaaataacca agactaccat gtcagtagcg ttagcaccac
     6301 gaacacgcat attacagaaa gccgcgtgtc caggagtatc gataaaagtg ataacgccag
     6361 ttttaccaga ttttgtcttg acggtagtct gataagaatt tatatgttga gtgatcccac
     6421 cagcttcttt tttggcaata ttagcactac gaatcttgtc tagaagagat gtcttgccat
     6481 gatcaatatg gcccatgaca gcaacaatgg gtggacggaa tgatgatgtc ataatatttg
     6541 aatcctaaac tctaaattct aaatcctaaa attaaatttt tattttgcag cttggacctt
     6601 gatatccagc ccacaaagaa tgccagcgag acgaacattc tcaccaccag caccgatagc
     6661 tagagctaat tgtgattctg ggacgtcaac tacagcttgg ccgtctttga tagaaacgac
     6721 tttggcatct tcggctggag agagtgcatt gacgacaaat tgattaaggc tcttggcaaa
     6781 agcgacaacg tcaactttct cattactagg aagttcgttt aaaacagctt ggatacgtga
     6841 acctttttga ccaatacaag aaccaactgg gtcaacacca ggttgactgg aagaaacagc
     6901 taccttagtc ctttctcccg acgatctagc aactttttca acaacaacgg tacgattacc
     6961 cacctcagga acttctctag caaatagttc tttgataaag tcaggatgac gtcgggaaag
     7021 aattatgttt ttgcgaccgg tatcatcagt ttcgatagaa gtgataagaa aaagtttttt
     7081 ggaacctaac ataagattct cattacgaat ctgctcttca gtcggacaaa caccttcggt
     7141 cttaccaata ccaacactca tcttgtatga atcgatatga agaacaacac caggaacaag
     7201 agtaccgact ttgcgttgat aatcaccaac gactttatcc ctctctgcct cacggatttt
     7261 ttgatcgata acctgtttgg cagtcatagc agcaatacgg ccaaaaccag gaggagtgac
     7321 atcggtacgt ttcttgtctt caactttgaa aatagagaat gagccagagg ctggagtaag
     7381 ttcgacttca aatgaatcat cttcagcaac gataatgcca tgctctttct gatcacgctt
     7441 gtaggccgaa attaaaccca tttcgatagc atctaagatc tcttgtggat caatatcgcg
     7501 ctctccagca atttgagcta cagctgcggc aaattcagtt tttggtagtc tttttatgtc
     7561 cattttattt ccttttgata aataaaaaag gggacgtcac gtcccccatt ccaagtatca
     7621 cagattaact agagagatta tagcacaaaa atcatagtcc taagctcttt atcaattctt
     7681 tttgtttggc ggtgagcttg gtaggaatag tgacagttaa acggatatac atatctcctt
     7741 gtccgtaacc attaacatcg cgaatcccct gcccgcgaag acgaatgaga gtgtttggtt
     7801 gagtccccgg tttgacacgt accttaagtt tttccccaag tgtaggtact tctacatcag
     7861 ttcccagagc agcctcagag aaagatattt cgacattaac atagacatcg ttaccatcac
     7921 gttgaaatat tttgtcggga aggacgtcaa tataaagcat gaattcgtta aagcgaatac
     7981 gttgaccgtc gctaacacca ccagggattt tgatttttcg acgcttaccc tcgatctcaa
     8041 cttctttctc ggcgccgttg gcggcatcta agaaactaac ttgaatttta taagttggga
     8101 tctggcgggc acgctgacca ccaccaaagt tactggatcc aaaaaattgt tcgaaaatat
     8161 caaaagggtt actaaatcca ccaaaatcaa aatcagcacc ttgaccacca gcaccacttt
     8221 gccaggtgta attaaaagga ccgttttgtt gggtataagt gtgaccacca aaaccaccgg
     8281 caccagaatt tggatcaaag gcggcatgac catattggtc gtaagcagtt tttttctgat
     8341 cattactgag gacttcgtag gcctcgttta tttccttgaa tttttcctca gcatcggggg
     8401 ctttattacg atcaggatga ttcttcatgg ccatctgacg ataggccgat ttaatctcgt
     8461 ctttgctagc acccttctta actcctaata cttcataaaa atctggtttc attttaatat
     8521 ggattcaccc ttaccaataa acttttcaat tccatctttt atttgtgatg gttgttttgt
     8581 cttatcgatt acaaaaacca tgcataaatt accttctatt gtgacggggt atgggtatac
     8641 gccatccaaa tttattcctc ttctcaacat agtttcctga taagcattga cttcttctgc
     8701 ggtgataccc gtctcaaatt ttaaatattt gtgatagacc tctgaacttg taccgagtga
     8761 ctctctatct accccctccg ttgataaaat tgaagcagct ttttctggca tattttttaa
     8821 atttgtagag acgccatgcg tggcgtctct acgattatat aatgtttttt tagtagagac
     8881 gcgattaatc gcgtctctac catgcgagtt aatttattta accacttcgc cttcttcggc
     8941 gtctttcttg tcatcagatg gcttagcgtc gtcggctttg ggagcttcac cttctggtcc
     9001 gccagttggc ggagtttgct gggcatagac agcagcaccg atagtttgaa gaatttgatt
     9061 tagatcttcg gaagctttat ccatttcgtc ttttttgaca tcagctttag ctaaaatgtc
     9121 tttagcagat ttaatctgct cttcaatctt ggccttgtct tcgggcttaa ctttatcacc
     9181 aaaatcttta actgatttct caccattaaa gatggtggcg tcaagtttat tgcggctttc
     9241 agcgagccct ttcttttcct catcatcacg agcatgagcc tcagcatctt ttttcattgc
     9301 ctcaacttca gcatcagaga gattggtagc attttgaata gtgatctttt gctctttacc
     9361 ggtaccctta tccttggcag aaacactcaa gataccatta ctatcgatgt cgaaagtaac
     9421 ctcgatttga ggtgtgccac gaggagcagg ggcaatgcca tcaagaacaa aacgaccgag
     9481 tgatttatta tcaacagcca tcgggcgctc accttgaaga acattgatct caacttgtgg
     9541 ttgattgtct gcataagtag taaagatctg tgattttttg gtaggaacag tggtatttct
     9601 gtcgattaat ggagtacgga caccaccagc ggtctcaata ccaaaggtta atggagtaac
     9661 gtcaagaaga acaatgtctt tgacttcacc agtcaaaaca gctccctgaa cagcggctcc
     9721 aatagccacg acctcatctg gattaacggt agtatcaaga tctttaccga agaaatcttt
     9781 gacaacttgc tggactttag gcatacgagt catacctccg accataacga cgtcagtaat
     9841 gtctgattta ctgactccag catctttgag acacttttca actggaccaa tagttttttg
     9901 aattaggtca tcgactaatt tctctaaatc agtgcgagtc aacttaacag ttaagtgcaa
     9961 aggattaccg tctctttggg taataaaagg tagattgata tctgactctt gggcagtaga
    10021 cagttcgatt ttagcttttt cagcagcctc acgaagacgc tgaagagctt ggggatcttt
    10081 atcgagatca acaccttgat ctatcttgaa atcgtcaacc aatttgtgaa gaattttttg
    10141 gtcaaagtca tctcctccta agaaagtatc gccattagtt gccttaactt caaagactcc
    10201 atcaccgatc tctagaatag aaatatcaaa tgtgcctcca cctaaatcgt aaacagcaac
    10261 cagacctttc ttcttcttat caagtccata agctagagca gaagcagtcg gttcgttgac
    10321 gatacgctgg acgtttagac cggcaatctc tccggcttgc ttggtagctt ggcgttgaga
    10381 atcatcaaaa taggcaggaa cagtaataac agcttcagta actttttcac caagatattt
    10441 ttcagcgtca gctttggctt tttgcaaaat catagcagag atctcttgtg gggtataaat
    10501 tttaccgccg acttcaacac aagccatttt gtccttacct gcgacaattt tgtatggagc
    10561 aatcttgatt gttttaacta cttcaggatc gtcaatacga cgacccataa gtctcttgac
    10621 actaaagatg gtgttaagag gattgagcac catttggcgt ttagcaaggt caccgaccaa
    10681 ttttttaatt ggttcgacga ttgaaggaat agtattgcga ccttcagagg taaagataac
    10741 tttggcagta ccaccttcca taacagcaac gcaggagttg gtagtaccta agtcgattcc
    10801 gattactttt gatgtagaca taattttaaa taatttttaa tttataattt ttaatttata
    10861 attttaccga caataacttg agccggtcgg ataacttgac catttaataa gtagcctttt
    10921 ttgtggactg agagaatttt gttttcttct ccctcactgg tactgacaca ctccatggtg
    10981 ttgtggtcaa aaatctcacc ctcagtcttt atctcttcaa gaccttcgtt tttcaaaact
    11041 gagacaaatt tatcgatggt catcttgaga ccttcatctt tgagatgatt ttgagtcaag
    11101 tctagatcat ctaagacttc gatcatctta gaaataatgg aaacagtcgc catagtaaca
    11161 aacatctgac gctgagactc aactcttttt tcaagattag aatagtcggc cattgaacga
    11221 gctaattttt cctgaagttc ggacagttta gatagatctg ttttttgtac ctttttggtt
    11281 ttcatgaaaa aaattaacgg ctaagagtgg acaataatcc agcaaaatag tcgacagttg
    11341 ggataatttg ctggtaacga caacgggctg gaccaacaat tcccaaaatg ccacgatgtg
    11401 gacccgcttc gtatgtttga tagataaatc cacaaggctc aagatgctcc atgccaagat
    11461 cttcacctat taataggtga accacctcgt cagaagaggc gccgttggcg ggaccaattt
    11521 tattgataat gtcaaaccag aaagtggtgt tgtctactaa ggaaagaact gtcttagtaa
    11581 catcgatatt gaaaaactct ggctcttcta aaagattact ggcaccataa ctgtacattg
    11641 aaccttggtc ggtagtggcg atagccatgg agcgagtgcg gaaggcgagc tctcgagttg
    11701 tttctttaag aagtctttca aattcattgc gataatccca gactttttct ttggcaccta
    11761 tttcctcagt aacagacaga cctttgggtg tcattaaatt acgaacgtaa tattttagac
    11821 ccatggaggt ggggccgcga ccagcggaaa gatgggattt tttaaggtag ccaagttgag
    11881 ttaaagatga catctcgtta cgaatagtag ccgaagagat gccgaggttg tatttttttt
    11941 ccagactttc agagccgact ggagcagccg tttcgataaa ttcctcaaca atacacttaa
    12001 gaatttttat ttgtctttgt gttagatcag ccatgttagc aatatattac ccagactgct
    12061 agcgagtgtc aagggggaag ttgacgtaag gctcataata ccttataata tagttatggg
    12121 acataaaata attgaaacaa gaacattggg ttacactgac ttatcaacag gagagaagag
    12181 agcaattggc cagattatta ccattgagaa tgggcaaaaa aaagttctac ccgtagctgg
    12241 aaaaactgaa ccaggtgaac ttcccggcac tggtggtctt accgacaact tgtcttttgc
    12301 caatgcaatt gccagaatga gtaagtgtcc agttaggacg tttgttgact tagaccaact
    12361 tgaaaaatac agaaaggcga aaggaattga tcatagattt gacctagtga agttgtacga
    12421 cttaaatgtt ggttaatctg cttgggtgag atgtcatcta ttcggtggta attattgact
    12481 tagtctgccg ataggtgtta gtaggagaaa ttgactatac acatttgttg tgtataatac
    12541 ctaacattat ggaacataca ataagaactt tgatcaccgt tccaaaggga aaatctacca
    12601 aagttctttt ggacgcatcg tgcacaagtg gtgtcagcat aacaggatac agagagaatg
    12661 ccccatacgg agaagtacat atatcactgg aaggaaagcc atcagaccta agaaaaatta
    12721 gagaaaacac cagagaggga aaagttcgag gatgccttga aggatgggac gcagtcaatt
    12781 ttatggtcgc accatccagc gcgttaaaac aaaggcgata aggcttaaca aaatcagatt
    12841 aacagaagta aatatttgaa gatattgcat taatataaga ccaaagagat agctcgaaac
    12901 aataattgat agccggactt taagtaaaaa taaattggta gagaaaaaga taacgcaaaa
    12961 caataaaaca aaaaatgtac caatagcccc attgctgatc ggaggaacaa ggttaataaa
    13021 ggccaaaaga gaaaacccaa acacaaaagt aaaaagttta ttgagtaaga gcattggtcg
    13081 aagtggtacc ttcaatattt attttatcga cgactttttg aagcttcttg gtgcgagcag
    13141 aagcgacttc atcatattgc ttatgaagac tgtctatcct gtcccctatt ttcacaaact
    13201 cttcgttata tttttcaaac tctgtaccaa agattttaat ttgagtaata attccttgga
    13261 catttttctg aaaacggaag ttttcatagg cctgcttgac catacgtaaa atagcggtaa
    13321 aactaaaggg gccagtcata acaactttac gatttaaagc atcctgccag acgtctggaa
    13381 aggattcata aataaaacta aagatcattt cattggggat aaaaagaatg acaaaatcga
    13441 cagtgttgtc ttcgggattg atgtagttac gggaagtaac atcgctaatt ttctttttga
    13501 tatcttgctc aaaaagtttt ttaaattgaa ctttttggtc tgaatcggtc gcctcactca
    13561 tcttaacgat attggaataa ggaaatttgg agtcgatgtt aatcttggtt ttatcgggaa
    13621 gatagagagt aaagtcaggg cgggcatcac cagtagtttc ctgacgagta taatcggtac
    13681 cgataacaaa gccagacatt tttaataaat cctcagcgac tttttcacca aaagctccac
    13741 gctgttgatt gttactcaat actcgtttta gaccttcagt agtgaccgat aattctttgg
    13801 ttagcttaac attattttca atagccgaag ccaggctggt aaaactacca acacgctctt
    13861 tttcaacttt agataaattg tcactaatat ctttgaccat cctttcgatg gcatcttttt
    13921 tgttggaaag atcagtgtga attatttgtt tttctttgct aagctgctcg ttatttaagt
    13981 ttataagagt ggagatggcc ttattaatat cgatctctac tggttttgcc tgtttttgga
    14041 ggtaaataaa ataaatcagg gtggcgagaa taataaggag aagaatataa ataagggtca
    14101 taatttattt taccccagta gttccaaaac cacctcgatc aatattttct aacttatcaa
    14161 cctcaaggat ctcaatcatt tctgttttct caactaaaac ttgagctaga cgagcaccct
    14221 tctcaatttt gatttcatga tcagtgtgat taataacggc aaaatttagc tcgtcattgt
    14281 cgccacaata atcttggtca ataagtccta acccgttagc tggagttaaa ccaagttttt
    14341 gagtcgagct acgagacatt aaaattatgt gatagccttt tggaagctcg atagcaacgt
    14401 ttaaaggaat aatagtaact tgatggagtg ggacagtaat gtcgatacga gagcacaaat
    14461 caaaggcagc cgcaccagcg gttttgtagg ctggtaaagg taaacttttg tcaaagcgtt
    14521 tgatttttat ttgcatttat tttttaatta ataaagtgct tttataactt gcaacggtct
    14581 ctcataccac tgaagtgatt ctgtctccat cgatttttta atttcatcaa tactcatcca
    14641 cttagtgtca cctatttctt ccgcaagtgc atttatttct tggcctggct ttaatttgta
    14701 ctcaaaaaca ttgaaatact ccccggaact aggtaatttt actaaaactt caccgacaaa
    14761 tttaagttct gactcgtcaa ttgataatcc aagttcctca ctaatttctc gaactgcagt
    14821 ttcaatataa ctcttaccaa aattaacgtg tccgccaaca ctcaaatccc aacaatttgg
    14881 atacaaatct ttggttggac ttcttttctg aagcaaaact tcccctttat cgtttttgac
    14941 taagatataa atagtccggt agaaaactcc cgaggtagtg tgagcagtgc cacgactaat
    15001 acttccaaca acttggttat gttcatcaac ttgagttaat agttcttctt gaggatctgt
    15061 ggtattcatt tctaaaattt taaacttaat aattcttctg gtttgttaat caaaaaatct
    15121 ggactgacgg aagagagcgg gacgttgcct tcaaaacccc aagtgacagc gacagacttt
    15181 acaccagctt tgtgggcagc aataatatcc cgtgattcat ctcccagata ataaacgtcg
    15241 tttttattta tttttaattt tttaataatt ttgtttagtc caatttcttt accaaaatat
    15301 gaattgtctt cataaacacc actgaaatat ttatcaatat tatttctaac taaatactta
    15361 tttatggttt cgacggtatt actactaaaa actaaaagtt tatatttttt tgagagtttg
    15421 ggtagttgtt ttttaaagtt atctaccaat gggcaatcta actgatgatc ttttattttt
    15481 gattttatat ttttatacaa ccacaataat tgaattttag aaatttttat ttctttcatg
    15541 aaatttcgaa caccagtctg ttttaagtat tcgattgaat attttgtcaa atcaatgttc
    15601 ttagatttca atgactcagt cattatctga ataactagtg gcagatcgtt aacgatagta
    15661 ccgttaaagt cgaaaattag atacttcatt tgctatgtaa taaataatgt aaattctttt
    15721 tgtttttaca attttcacca atatggtaaa agtcttgatt aagaattttt tgataaatat
    15781 ctttgatttg ggacttcaga ctttcgagat gttccggggt gatttcatat tctttacgaa
    15841 gataattatt ttttgatctt gatttttcaa caaaatcgat agtagccttg acgaccgtat
    15901 atttaaaatt gggatcgaga gtacttaata atttgtaaaa aactaactgc cgaaagtaat
    15961 cgccatcttg acttaattct ttgtatttac catcaggatt accggtctta aaatcaacca
    16021 cttcgacatt cttatcgtca acaacattaa taagatcgat tttaccggta agggggatat
    16081 cgtctagatg aacatgatca tgtttaaaat caaaatcaac aatactatta cttgaagtaa
    16141 tattgtaatt ttttaaatat tcgtctaaaa gtttttgacc tttagtaagg ctttcttgat
    16201 aatcaccttt aggtagttgc tcatgttgca aaacagaaat aaattttgag tgaggatctt
    16261 tgccggtata ggcatccgag agggcggcgt gaatggcagt accaaaacta ctaaatttgt
    16321 cttttgactg ggggactctc agaatagtgt tgtgatagaa acaaaaagga caacgaagat
    16381 atgaattaag atgagtgaca ttaaaaatat aatcagtagt caaatattga tgtaaatagt
    16441 tttctatact tacaagagat ctagaggatt taagcttcgg agagaaaaga tttttaagag
    16501 atagctcttt aatatcagag ttgaggttag ttgtctcgat aagttttggg tcaatttctg
    16561 acagaaaaac tgaaggcagt tggtcacggt tggaattact tttacttgaa taggagagat
    16621 aaatctggga cttagctcta gtaagagcaa cataaaatag tcggcgttcg tcctcgttgg
    16681 cgtcggcgag ttcaagtgaa acctcagtat taataatacc aggaggtaat ttaataactg
    16741 acctgtcggg attgttgccc cactttttat caagacactg gataacaaag acatgagaga
    16801 actctaatcc tttggcttta tgggcagtca ttagttgaac agaagaagaa atgttggtgg
    16861 tcaatggtgg agtaggtaag ggaatattat tttcgatgta taaatccagt ctgttcaaaa
    16921 aatctgacaa agacatatcc ttctctaaac aaagttgctt aagaagacta taaaaagtat
    16981 gcagctggtt cagaacagaa atattgtttt ctttaagaca atagttgaga aacttgaatt
    17041 tacgaatagt aatattaaaa aatttgtcga gactatatct ttgcagtcga actctggctt
    17101 tggcgagacg aagattaaat tgttttagtt ttttgtcaga tttttctgat aatgattcat
    17161 ggcgaatatg gtgcaataaa tctaatggat caaaattaac aaattctgaa gagagcacgg
    17221 ggtaaagaag tgttaagtca ttagggtcgt taatgagttt gagaaggtta ataagatgaa
    17281 gaatgggctt actagtaagg atgttttgat cagaagcaag gtaaaaagga atatttaatt
    17341 gatttaattg ttcaaccaga tcggtactat cactgttgtt acggtagatg atggcaattt
    17401 cagaggcggg tacattattg tcaagaagtt ctctaatttt agaagccacg agatagtttt
    17461 cggaaatggg gctatcggcg acagttaaat taataggatc aggatcgata gtggtgacac
    17521 tctttaaact tttatcgata ttttttataa gattaccaag acgattttga ttgtggttga
    17581 taactgaggc agctgagtcg agaattagct gatggctgcg ataattatta ataagaacaa
    17641 ttggatcgac tttgtattta gtgagaaaat tttggagatt ctccaaagat gctccctgaa
    17701 aacgaaatat ggattgatca tcgtccccta caacaaagat attggggttg tcaaagtagc
    17761 tacccagaag atcaataatt tgattctgag aagagttggt atcttgatat tcatcaacga
    17821 gaatatattg gaatttttct tggtgttcta gtaatagatc tggatggtcc ttaaatgctt
    17881 gaaggacaaa aagaatcatg tcgtcaaaat catagatgcc acggctcttg agagtggatt
    17941 gatagccaga gtaaaggact agtaattctt cttgtttggg gatgtttttg acgaaatttt
    18001 caattagatg taagagagca tttttgaaat tcacgaccgg agatttggca gcgccgatgt
    18061 cataaccacc gttttgaaaa agtgacagat gataagtaat aaggggtgtt tggggaagag
    18121 cagaaactag cgagagcaat tgttgttcta aacttttgtt agcacgaagg agcttaagct
    18181 ggtcataaat atcttttgat ttgtcaacga aatcaatttc tgaatcaatt aattttcgaa
    18241 aagtatcggt agtcacattc tctcttttaa gagtctggat ctgagaactg atatcgcgct
    18301 gataataata tttatcaccc catggtttga gggtggcacc gtcggggagt tggtcgatta
    18361 actggcgaaa gatttctatc tgctcaagtt gatcgagaac tttagcttct ttggcaagaa
    18421 taaaatattc tggatgtgat tgaataacct cattacaaaa agaatgaaag gtgtgaatct
    18481 tgagtgaata agcggtagag ccaatgatgg aaacaaggcg agagcgcata ttaaaagcag
    18541 cagaatcagt gaaagtaaga cagagaattg atgagggtgg tgtctgagtc ttgttgagga
    18601 tattagcgat acgtagggca attgtttgag tttttcctgt accggcacca gcgataacca
    18661 taactggacc ttcgagagta tcgactgctt ggcgttgggc tgggtttaac ttttggtatt
    18721 cggaattaaa gtctggcact gttaatagta taccgataaa ttaaaaatga taaagttata
    18781 tcctatagtg tataatannn nnnnnnnnnn nnncagagat agttttgcga caaaactggg
    18841 gaactatgtg tcaaacaggg aaaggaatta aggtatttgt aacggagtgt gtacctagat
    18901 ttgttggtaa tataatacct gatgcaaaag acggttgcag aagaattgcc gaagtggcag
    18961 gaaagataat tggtggaact ttgtaataaa caggctttta gattttgttt atattggctt
    19021 ttttcataaa gctgatgacg gcagggtcta caataacgtc attatgggtg agagggcgtt
    19081 taagatataa agtctccaga ctcttgcagc gagacagagc cacataagct tgaccgtgag
    19141 caaaagctcc ccgacccata tcaattacta ccgaagtata agtttgacct tgggatttat
    19201 ggacagtaat ggcccaggcg agcctgactg gaaattgagt aaaactactg accacttctt
    19261 cttcgatttc tcttttttcg gagttaaaag aataacgaat tttattccat gtttctttaa
    19321 caactgaatg gacttgaccg tctatattaa cctttatttc attatgatca attgactcga
    19381 tagtaccaag agaaccatta acccaacgtt tttttgaatc attttttaac atcataatct
    19441 gagcaccttt cttgagaact aaactacttt cagtgggata tgaagaaacg tccattttgc
    19501 cgtcgataat tgcctgataa gaaaaagatt ggctagtaag cgaattcagt cgagaataat
    19561 taatctgaaa ggcggtggcg ttggtggtgg ctagggtaat cacgccatcg gcaggacaac
    19621 tgtcaaccac acgacgattg agtgaattga gaagatcctc ggtcatatca ccgtagcgaa
    19681 gtgaattgag aatagattta aaatcgtcgt ctgtttgtct aaatatagta ttcagctcat
    19741 agatttctag atcagtacca ttccaagcta atgaattgaa gaaataaaaa ccaccattta
    19801 aatcttgaaa ataagaatgg agctctggct cgttaataac tggtggcaac tgatagagat
    19861 caccaaacat taataactga accccaccaa aaggaaggtc tttatcgcga gcaagacgca
    19921 gcataaagtc aatgccatcc ataagatctg ctctaaccat agagacttcg tctataacaa
    19981 gagtgtcaat gtgttttaga atcttggcag tcttggcaga tagacgaaga cttctagtat
    20041 cgataaaaga tggtggaaaa agaaaaagag agtggatagt ttgaccaccg acgttaaggg
    20101 cagcaactcc ggtaggagcg acgacgataa ttttcttttg ggtatttagc ttgatgtgtt
    20161 gaagtagaac tgatttgccg gtgccggctt taccggtgat aaacatatga ttctcagtgt
    20221 tttcaaatcg gttgaaaagc ttctgctgtt cgatactaag agtaatgtcg gagactttta
    20281 gctcttggag attaagtttt aagggtggaa gaggcataaa gaagtatatg cttattgaaa
    20341 ttaaaaattc aaagaataca attaattctg aaggttatca atattaattt aaaaaaatga
    20401 aaaaagctgt agctaaaaca aaaaaggtcc tgcccgcaag tagtaagaaa aattcaatac
    20461 tatttttggt gagtgcactc gcatttagtt tgttcttatt agtagtagtc ttaatggcta
    20521 tgggaaactc acaagataat aggtctgatg ctgctaagaa aaccccactt acaccaaagg
    20581 tcaatttgtt caaaaacgct ggtactggtg gtaaggtcca accagctgaa gtgaaacaac
    20641 aaccctcaac aaatactaaa gacacaacta agaaagtagc accgaaggtc gaaactaagc
    20701 cgtatgttgc gccagacaag ggatcaagat agtcttaatt gataaacaaa aaaaaccggg
    20761 gaaatttttc tccggttttt tttggactac tttataataa caattctgtt aataacaata
    20821 taggtctgta aaagtgaagg attgctgact aagatgatgt aagtgaagga gaacagagta
    20881 tcaatcagtg aagtattatt tgtaataagg ggtggacccc ctaaggatat aaacccggaa
    20941 ttagcaaaga gaggaatagc agctcacaag aaaattgaac aagaggcgag agaagggaaa
    21001 atacctccat ctctagccag agcagggttg agacctccga taactcccga aaagacagtg
    21061 catggaaatg taggtggtac cgaactttct ggcaggattg accttgtagg aagtgatggc
    21121 atggtagtag atttaaaacc gggagctgat attaaaggaa ggcatttatt ccaagcggca
    21181 atggctgcta gggccgacag aagcggtaaa gcagccgtac atctatatgg ttcaggaagg
    21241 acgtatgtaa taactgacgg tggacaaagc gctggagaag agattggggt agtggtaaca
    21301 aacgcgagga gaatattaga aaatgaagca gagatgaaaa ataagcgaaa gacactgggg
    21361 ccagtgggga tgcagcaaaa gggcacggag acggtgaagc ttagacaaga gttagaaaaa
    21421 aacttaggtg atgcgatgga caaaataaaa agtaagttag ttaagtacca ataacttttc
    21481 caaaacttct ttggcgactt gaggattggc agaaccacca gatgatttca taacttgccc
    21541 tacgagaaaa ccgatagaca tggggttttt ctgatagtca gcaacagctt tggggttatt
    21601 ggcaataaca tctttcgcga tttcttctaa tttattggtg tcggaaactt gaaatagatt
    21661 ttgttgcttg gcgagaatta gtggatcaac ttgtttgtcg tatgattcta agaccaattg
    21721 tttgatggct gaggaagata attgatcttt gttgtcaaat aaatttttga aatactctgg
    21781 attaatttta tcgatagtta tctcgattgt cttaagagga ccaattaata ggttagccac
    21841 aaatttagcg taaacagaat cactatcagt aattatttta tcgaatattg aggaaaagta
    21901 tgaatcttca gatagaagat tagaatcgta atcagacaga ccgagagttt tgtacttagc
    21961 tatcttagct tcgggcatct cgggcagagt ggcgcgaatg gcctcaactt gttcttttga
    22021 aaaaattatt ggagggatat ctggctcagg gaaatagcga tagtcagcac tcccctcttt
    22081 ttcgcgttgt aaaaaagttt gctgtttatc agcatcccaa ccacgagtgg tcttgttggt
    22141 cgaatttttg gtgattttat cttgagaata ttgttctgct tgacgatcaa tttcatattg
    22201 aatggcggtc ataacgccag tgagtgaggc aacgtttttt atctcgacaa gtggagtata
    22261 aaactcgtcg tcgatacaca agttgatggt tggctcacaa cgcatagaac ctttttcaat
    22321 atcggcatca gagataccaa gataacgaac aatctgttgg attttaagta gatatgtctt
    22381 ggcatcttca ggtgatttga tatctggctc ggagacgatt tcgacaagag ggacaccgga
    22441 gcggttgaaa tctaatagag tctcaccatt tacatggaca gacttgccgg tatcttcttc
    22501 ttgatgaaca cgattaatac ggacatcttc tagcttaccg ttaatacaaa aaggtttttg
    22561 aagttgagat atctgataac tcttggcgag atctggataa aaatagtttt tgcgttcaaa
    22621 aaaagtttct tcattaatag tacaacctag ggctaggcct aatttgatac accattcaca
    22681 ggcttttttg ttggggacag gaagtgctcc ggggagacca agacaaactg gacaagtatg
    22741 agtgtttggt tcggtatgga aatgctcggc gtcacaacca caaaacattt tactttttgt
    22801 ttttagttct acgtggactt cgagtccgat aataggagta attttcataa ttgaggtttt
    22861 tgggtgtgat aatcagttaa cttttgatac tgatggccga gggcgagaag aagttcttcg
    22921 gaaaacattt taccgactat ctgagcacca atgggtaagt tatttttagt aaaaccacaa
    22981 gggatggcga gtgaagggac accgacagga ttttgagaaa cagtgtaaat atcagccaac
    23041 aagttttgaa gcgggtcact taatagctca ccaaactttg gcgggatgtt gggcataatt
    23101 ggttgtaata aaacatcaca ttgggacaaa gctttcttat attcttcaat aaatagagtt
    23161 cgtacttgtt gagctttttt ataataagca tcgtaataac cagctgacag agcataagtt
    23221 ccgataagaa ttctgcggat agtttcgtcg gtaaaattat cacgacctgt accatagcga
    23281 actccatcat aacgagccag gttggaacta gtctcgctag gagcgataag ataataaact
    23341 ggcaaacaaa gatcgaaaag tggcatagaa atttctttga tagtagcacc agcagtctgc
    23401 aaatctgttg aaactttcaa gacggcttct tttatctctg ggtcaagacc gtcaccgtag
    23461 aactctttgg gaataccgat agtaacattt gaaagatcta gatttagtag tgaagaatag
    23521 gcagggacag aaacaggaga agaggtggca tcgtatttat cgacaccagc aatagtttca
    23581 aggacaatag cacagtcctc tactgactta gcaactggac cgacagtgtc aaaggatgaa
    23641 gcgtaggcaa tagcaccgta acgggagaca cgaccataag ttactttcaa accggtagtg
    23701 ttagtccaac cagcgggatt acgaatcgaa ccaccagtat cttcaccaat agcaaaaact
    23761 gccatacgag tagccacggc cacagctgga ccaccaccag aaccaccagc aactcgagtt
    23821 atatcccaag gatttttaac tggaccaaaa tcagtatttt ccgacgaacc accgtgaccc
    23881 caagcatcca tattggcttt accaataaga atggcaccag catctaataa ttttttataa
    23941 atagtggcgc tatatggagg aatgaagttt tctaaaactt tagaagcagc agtagttctg
    24001 acaccagtag tagcgaaaca atctttgaga gcaaaaggta gaccggaaag tggtgaagtt
    24061 aattttacgt cttgagcttt ttttaaagct tcttctcgag tgacaatagt gataaaggca
    24121 ttggtcaggg aattatattt ttcaatattg tcgtaacatt cgttaactaa gtcaacagaa
    24181 gtgaattcac cggatcgaag tccagcagat aattcggaaa ttgatttgtc gagtagcata
    24241 ttattttttg atggtggcag aggtgacgaa ataaccgtta ctactttggg gggcgtttga
    24301 cagagcttct gattgggtaa acatacgttc ggtatcgata atgtcttcac gtaagatagt
    24361 gtttagaccg gttgtttggt aagtagggac aacattagta gtgtcaactt ttgataaaga
    24421 ttcaaaatgt tcaagaattg gctcaagttg agaagcaaat tggtcaactt tctctgtagg
    24481 aatattaatt ctggctaatt tggctacctt aacaataacg tcactggtga taattgagga
    24541 catagggtat tttaacgcaa aaaatacctc aatgtcacaa cctatttaca acagggaata
    24601 tacctgtttt gtttgaggaa cagacacata ggtctgttcc tacgggataa tttaaaatag
    24661 attgtttctg tctttatccc agttctttgg attatctttt atataaattc ttttagccca
    24721 caattctttt tcagatctaa taatacgatc atgaaaacgg ggttgccata actgcttgtg
    24781 gaattttggc caattgtttt gtttaacgtt ttttatgtat tcattagtag tcatggtttt
    24841 gaaccattga atagcagatc ctaataaacc tacattacca aaaatatttt gatttattgt
    24901 aggaacagac ctgtgtgtct gttcctcttt ataattggat gtatagaaag tttttttcgc
    24961 atcaataatt aatatgccat gaaagtggtt tggcataaca acatatttat ctgtctttat
    25021 tgattgaaat tttttaggaa tttcttgcca ccacttttca accatgaggc caataacatt
    25081 tagatcacca atcgattctt ctttattatg agaggaacag acacataggc ctgttcctac
    25141 tgtgggatcc gtaacgcata ggtctgttcc tacgggattt tttgttatat ccctaaacaa
    25201 attaaccttg tctttggtac aaatggtgat aaagtaatac ccactattgg aataatccca
    25261 atcttgtctt cggtattgtt tctccatttt aaatggataa tttacccagg gcatagatta
    25321 ataataatct caattaaatc cttgttaaag gaacagaaac ctacaagcta ataaaaatat
    25381 gtatctttga ggaacagaca cataggtctg ttcctacggg gaaaaacaga aaataattac
    25441 ctcatcaatt tggctttgag gcgaagaaat tgccatctat atttgtcaac cacattgtag
    25501 agcttatata aactatgatt aataacaaaa tcataagtgc caacaaattg aacaagctgg
    25561 cctccgtaac cttgcttaaa acggtggaaa ccgacaaaac cttcatcacc ttctttagct
    25621 tctgggccaa gactacccca catatcaaaa ctcttaagac caagcgactt acccaattta
    25681 atacactccc acatcaacag agtcggcgcc atcacctcac gattttcggc aagtgatgcc
    25741 ccatacggat agaataattt atcgttgaag gtaaaaagca tgaaagcaga taaatactta
    25801 ccttgatatg aggccaacat aatacgaggc atgccagtct tcttgagact attccataat
    25861 tgacggtgat aactgattgt atgtaggtaa aaaccttgtc gcttggtggt gttaaaaagt
    25921 aagtctagat aaatctcaaa acctttatcg gtagacatct cctcgacttt gacaccatga
    25981 cgattggcaa ctttgatgtt atatctagtc ttggggtgca ttgactcaag tattttttct
    26041 tcaccttggg acaaatcaac aacaaacgaa tgtggataaa aagcaacttt aggtgaaact
    26101 actaagccag gaaaatgtgg cagctcggag acggtggtag gcgagttatc acgatcatag
    26161 accttgtgat atacatctgg ctcaatcttc acaaaaatag cattttcatc gacagcaatt
    26221 ttttttatat tattaagcat ctcttggtca atatctggcc cacgaagaat agtaccaatg
    26281 gaatagttta atttgggaat tttatggaaa ctaatagaat aagccttaat tattttattg
    26341 ttttcaaaga cacctaatcg atagactttg tggccttggc tgatctgaaa atctccccac
    26401 tcccatgtct ggacaggatg tttaacgact ttgttatatt caagtttttc ttctggataa
    26461 agtattctag taaccatggt ttagtatata tcaggatcgt aaactaattc attactattt
    26521 tgattgtctg agtgggagtc agatagaccc gacgacaact cgtcagaatc aagaaggctt
    26581 tgaggtattt cacttaaaaa ccttgatggt tcattaagag agctcttgcc aaaaaaaagt
    26641 cgtctggtgg cataagagat ataaagataa tctttggctc tagtgatgcc gacgtaacag
    26701 agacgtcgct cttcttctat gtcagaatct tctatcatag atcgagagtg aggcaatatc
    26761 ccctcctcaa agccaaccag gaaaaccact tcaaactcta gacccttgct accgtggagg
    26821 gtcattaagc gaacaccgtc acgattttct tttttcttat tcttctcttg ggctgaatat
    26881 tcttgttgaa ctagggctat attttcaaga aagtcgtttg gtttgggaaa attagtagcc
    26941 acggatttta actccttaat gttttctagt tttttctggt cgtcttcttc tttggggtcg
    27001 tattttgcca aataaccgga actgacaacc aatgactcga gcagctggat agaggtcaat
    27061 tcaccagtat taattgactc aagatgatga tttaataagg acttacgtcg tttacccagt
    27121 gccttttcga ctctcagctc ggagagatgg tcgtctctgt tggtagcata acgtaacata
    27181 gccacaacat cttttacctc agcccggtca taaaaacgaa ggcctccaat aagaacatac
    27241 ggtatggttt gtttgagaaa agcctcttcg agaacacggg attgagcgtt catgcggtaa
    27301 aggacagcaa tattacgata aggtatgtga agattgtggt gaataaattg gattttgtct
    27361 gaaacatatt gggcttcaga aagttctgag tcagcagaaa aaactttgat ctggtcgtta
    27421 gatttttttt gggtaaatag ttttaaaact ggatgagagg tgttgcgaga aataacagcg
    27481 ttggcagcat cgaggataat atcggtggaa cgatagtttt cttctaagtt aataaccttg
    27541 gtgtctttaa agtcggaagt gaaactgatt aagttgtgat agtcggcacc tcgccaacca
    27601 tagatagctt gagaggcatc ccccaccgcc gtgacttgtt gatggtcttg gcccaagagc
    27661 ttggttagta gatattggat ttgattagta tcttgatact catcaacgag gataaaacgg
    27721 tagacatcct gatatttgga aagtatttgg ggatgattga gaaagaggtc aatggttcta
    27781 tagagaaggt cattaaaatc taaggcctga tagtttgata atttttgctg atatttttgg
    27841 taaatattag caacgtttct accccaaaag cctgagctac tcaatttagc ttggtccgga
    27901 gaaatatatt tgttcttaca atcttcaata aagtagagaa cagaagatgg tttgaactcg
    27961 gtaggagata agtcaagatc gtaaaggata ttttttacta aagactcttg gtcgttagag
    28021 tcgtaaatta caaaattagg attgatacca atgttgtgac cgtctttcct cagaattctg
    28081 caacagaatg agtgaaatgt accagcaaag atgttgtcgg tagaagcttt agagctagaa
    28141 cttaataatt tgccgagacg ttctttcatc tctccggcag ctttgttggt aaaggtaaga
    28201 agaagaattt gagaaggagt ggcaattttc tggtcgacaa aataggcggc acgatgagtc
    28261 aagacacgag tcttgccaga accagcaccg gctaaaagaa gtaaagcgct gccagtgtaa
    28321 acgacagcct ctttttgggc aggattaaga ccagccagta aattagacat attttaaaat
    28381 tggtaaattt tggtgaggcc ggaagtaaat attactttag caccaagctg gataaaatta
    28441 ttctcattga gattaagata tttttctcgc tctttgtcac caacaaagat gtagttaatg
    28501 ctgtattttt ggatgagcga ctgtacttct ccaacattag ttgattggta aattctttca
    28561 acatcagttt gacgggaggc tggagcatcg tagctacccc tccagagcca ctcatggact
    28621 atccagccaa cgacagtggg caagccagta gcagaagaga catgattaaa agtggtgtag
    28681 ctatcgccca ctgcctcaag aacaactggt tgatcagaaa tattgtcatt aatccactta
    28741 acggcatggt aattgtcagg gtaacgttcc ttaaggtagt taaatccatt aagacctttg
    28801 tagtccttga ggccatagta tgatctaata gcaaaatagg gataagacag atgagcaatc
    28861 agaacgataa agaatatggc tttgtaaatt gcatttgacc taaaacggat aagaataatt
    28921 cctgaggcaa tggagtacat aataaatgcc tgataaacaa gcttaaacat agtattggct
    28981 cgtcgatgtt cgtaaatgta gatatctttt atataaccaa tctcggggat gataactaga
    29041 atagtggcaa cgatgatcag agatagagtg aataactcag aggtcttaag actgcttcgt
    29101 cgcagaactg ctcgtaatga tgtgatagcg aacggaagac agatgatcca gaaaccacca
    29161 tacaagacga aaagttgata aaaaggagtc ctggtgtcgg acaggcgaag cccttcagcc
    29221 atgggaataa aattcaagtt gaaaggtaag gtaaatagat accagaaaaa tataacgaat
    29281 attccgtgag taaaagttga cacaacagag cgactccata agaaaaaaga tacagcaaaa
    29341 agaagcccat agacagcaaa gtcccaagcg ttggtcataa aagctaatga caacaagaaa
    29401 cctaacgggg caaccaactt ccaactaaca gtggtcggtc gaggttgagt gttgaaaaaa
    29461 agaaagagaa tactaatgaa caaaaggatt atgggaatac cgttcatatg cccatgaaga
    29521 tcggcaacta caaaactata caaaggaaac tcatgaatag ttttatcttg aacgtctgga
    29581 tcaaaaccaa taaatctagt ggcatctggg taccaatact gattgactgc agtctgtgga
    29641 cttaatccac caacgataat gttacgactt attttataaa gaggatgaag attacctcca
    29701 aaagtgagga taatggctga gacaaacccg ccaataataa tatgctttaa agaaacgtgt
    29761 ttggccaatc cgctaataat gtttgaggcg agagaaaaag tagaagtaaa aaccatggca
    29821 caagttgagg caatggctaa attgtaggtg atggcagagt caaggctaga caccttggta
    29881 atcatggcga ctatcaattg tccaaaataa taatagttga taggaagacc agaaaaccac
    29941 atatcaactg gtggaaaata ttcacttcta agagcagagt tgacaaaccc ccaatccata
    30001 tatttttcaa gaccttcgat atcaggagca aaaccacgaa cataagacca gacggccaag
    30061 ataatgaaga agataatttc ttgaaaaaga aaaatcttcc acttctgtct taatgttgaa
    30121 gaaaaaatgc catagttgcc gtttttattg aggtacaaaa tatttgctat aagtaccaat
    30181 ccaactaaac aaagaattga actaagagta aaaggcgcga ctttgaatac tcccgagata
    30241 aacaaaacat aggttatggt taggatggag attgtcctgg aaaagatgta gcccttatcc
    30301 caaaaactgc gaaagatact gaaagtcagg ggcaaggaaa gaaggccaaa taaaaagtag
    30361 attaaccacc ataaaaagat aaagttgaag tcgatggtca tactctaaat ataaagttaa
    30421 atataatacg aacaaaattg gttagaggct ctggtagaaa tatactccga gaagataacc
    30481 aaataaataa cccaacaaag gcaccaccaa acatatctat tggataatgg aagccgacaa
    30541 tgactcggaa gacaagaagg gggacaacaa tcagaatgta agtagtgaca ggaaagacgg
    30601 ggtattgtag tagaaacaag agaaagaagg tggctttaat agtgtgacca gaaggaaaac
    30661 tgccagtctg ataccagtgg tcaaccagac caaatggggt cgggtctttt ctcttataga
    30721 gcggacgagg ccagagtgca tgtgacttac catatttttc gacaaactct ccgactaaag
    30781 tggcgataaa taattgccag aagacgttgg tgtcagactt gatgagcaag taagagatgt
    30841 aaaatagaaa aaaccaaatt agataattca tggcgatgac aaaaaactta ccaggggtat
    30901 tggactggag aatcttcaaa acatcagccg atggttgcca aaaaaaagga gtgcgaaatt
    30961 tcatatgata aagtagctat atgggtgcaa agaaaatttt gattggttta acatattttt
    31021 ctccaaacgt gtctggggtt acactctatg ctgtcatttt atatgaagag ttattaaaaa
    31081 gaaaatacaa agtaaagata atcacctcca agtacaaaaa agaactacct gaaaagacag
    31141 gtaatattgt aagagtgata ggatttgcta taggtaaagg gtttattatg cccacgtatt
    31201 gctggcaatc gctccgggag gtcagaaaat gtgatgtggt caattgccat ctaccatcta
    31261 tagaatcttt ttggctggcg ttatgggcta aggttctcaa gaaaaaacta gtgatcactc
    31321 atcactgtga atttggtata gatggacctt ggcacaacaa gctaatcggg ataattacat
    31381 atccaattca ttggctagtt tatttaatgg ccgacaaaat aatttcgtat accaaagact
    31441 atgctgatac ttcaattttt ttgagatttt ttaaaaaaaa attaatatat atactaccac
    31501 cgataaagat aaaaaaactc aaaacttccc ccaaggggac tcacttcgtc gcgcaaaact
    31561 caaaactcaa aacaaagaca gttggttatg tggggagaat tgcctgggaa aaaggtttgg
    31621 atgtactagt aaaagcgatg aaaaaagtcg atgccaaact cgtattagtc ggaccatata
    31681 aagagttggc cggtgacaaa acttatacaa agcttaagaa tgttgaaatg gtcgggcctg
    31741 aaaaacatga agatttaaac aaatattatg aagggtttga ctgcctagta ctaccgagca
    31801 ctaacaactt agagactttt gggattgtcc aggctgaagc aatggtgtgt ggttgcccag
    31861 tagtggcttc aaacttacct ggagtcagag tgccagtgag aatgactgga atgggagaaa
    31921 tagccaaaat tggagatgtg tctgatctgg taaaaaaaat aaatatagta ttagggaatg
    31981 gtaaagaata ttatcaaaaa aaagcaataa acttgtctaa ctttgattac aagaaaacta
    32041 tagatcagta tgaagagatt ttttaaaaat tagcacctta gggtggtcgt aaacagtgaa
    32101 agcctcttca gccacatcgt cacgaaaata aatgccggga tagttacagg tattaacaga
    32161 caaccaactc ttatcaccag gcatatctgt tggaccaaaa taataacact taccaatagg
    32221 taacctgaag cctggatagg aattaatttc tactaacttt tgaaaactct gtctccctct
    32281 aaatagatcc tggtagaacc tcgatgctat ggggtattta tctggaacta gtgggatcga
    32341 tccccacaga cgattggagg acattatcaa ataatcaact tcactaagct gctgatcaat
    32401 cttttgccat ttctcaggag tgtcaggatc ataaaaaggt aaagacaagc ttttgtaagg
    32461 agaatgaccc aaaggcaagg ggtcatccca atattcactt gagataactg acctagagga
    32521 aatgtgggca agtatccaat ttgaggcctg cactcgggaa tgaggtactg aatagatagt
    32581 caagaaagaa agtgtaatta caaactgaaa aatcaacaca ggtcggacaa aactgggtct
    32641 atgccagaaa gcaataagaa ctatgaacgg ataaactggt aagaagtatc tcattgtgtg
    32701 tgccatctga gacccttgag taaccaaaac aagaacaatc cacaagagag atgtccaaag
    32761 taaaaaattg tctcttagac ggcgaagtga aactaggaaa agaatggtaa tgggaagacc
    32821 tagaccaaaa aaaacaatgt tctgaagagg gaaaatgctt ggtgttttac ttaaccattg
    32881 tacggccggt gggaaccaac cgctcggatc agaaaatgat tttaaagttt caaggttttc
    32941 aataaacaaa ggactgaggt taaaaaaact agtaaaaaca tagggattaa agatgcgaaa
    33001 aaccagaagt gtaataggta agacgtaaaa aataatacgt aaaatagctg ataacctttt
    33061 ttgcttgaca ataaacaaca agaaaattgg agcaaatata atagcggaaa ttttggaaga
    33121 caaggctaga ccaaaggtga tggctgccgg tataaacaaa ttgtatgaca ataaagtaaa
    33181 ggtggctaag ataaaagtgg tgagaaaggt atcaacagca aaaaaatggg ataattggat
    33241 agggagaata gttagagcat aaaaaagaga gggcaggatt ctctttctag aaagataata
    33301 aagcaatata atgtttgttg aatcaaaaag tgcagacaaa aaacgtccca agataaccag
    33361 gtgttgatag ttgtccatgc caatgacaac ggccagagtt ttggttaaga aaattgggaa
    33421 agtgccatag acaaagaatt gatactgagg ataattatat ggatttagag gagaggaact
    33481 cgactgaaag tattcaaaaa ctgaagatgg taacttgata tcgttggtaa ccatggtcaa
    33541 aaaacgttca tcaggatgaa gatggttatc ctgatcccaa ttcaaaccat acaatctcac
    33601 tgaaaaagca agaaaaaaaa tgatcaagat agtgagtttg ttatctgata gcttcttcat
    33661 acctcttata aagcctaact gttggtcgat caaaaactga ccatgtctct tcagctaact
    33721 ctgggtctaa taaaaaatcg gtatttggcc ggaattcttt gacaagggta aagccaagct
    33781 ctccagagaa tagtttctcg taatacgtgg gcatgatacc gtttttgaag actcgccgag
    33841 aaggaactac gatgtactct gctgctgata agttatctag gagcttgtct tggtcgtgat
    33901 tataaaagtc gtgataataa tcagccgaag gtagattaac tacgttacct gactccgaca
    33961 tgacagtttg agaggatgaa acattttgtc ttaaccactg atctgacgtg atgcgagtat
    34021 ctgcaaaaaa atataaattc atgaaataaa aaccgggaac gatacatata actaccaaaa
    34081 taaaacgtaa taaacgagaa ccaactttgg caattaaaat cccagctaca agtggaaaaa
    34141 taaaaaaaac tggagacata aagcgtgtcc atttggtata aagttgtcca aaataaagta
    34201 cgtaaagaag taccggcaga agaagaatca tcgatttatg atttatgata tatgatttac
    34261 gaataagacc gaaaaagaac aaaataaata tggggaggcc ggagatgtag gggaaaatgt
    34321 gagtaaactg gaaaaggtaa ggaatagtgt ctttgaactg agtggtgtaa aaaacatcca
    34381 ttttccccgt agcgacttga gtctcgtatc tcattgagga gataaattct gactttgcaa
    34441 gtaaattata aggggaaagt agaaaagaaa aactgagtga taataaagta aatataataa
    34501 ggtgtactgg tcgttttagg ctgacgataa cgattggtaa aatgaaaaac aaagcagata
    34561 ttttagtggc aataccgata ccactgatga cggccgcaag taaatataaa cggccgggat
    34621 agctacttga agacaactta aacgcaagat agatatttga taaaaatacg agtatgagca
    34681 agctttctgt agtcccaaaa tgagatagtt gaattagtcc cggggtaaaa ataaaaaaca
    34741 acgtagggat aaatggcctt atggtgaatc tcttggaaat aaggtaaaag atatatactg
    34801 acagagaaga gaagacggct gaaaggagac gaagaacaat tatggaattg gtaaaagagt
    34861 tgtcttggcc aagcaatttc aaaattagat aacctagata tagtgggaac tgcccataag
    34921 caaagaagtg agggtgtaaa ctagaaatag acaagccaga taaggctgtg gccatgttgt
    34981 tctcgtccgg atgaaaaaaa aagccaccac cccagaccaa accgtaaaat ctagtgaaga
    35041 tggtagcaaa aacaagacta aagaacatag ttgatggtaa tgatcatcaa cccccaagaa
    35101 gcgacagcaa tgatcaaggg tatgtcagta gcgatggttc tctctggacg ctcaccttct
    35161 tgcatttcaa aaataatttg accatacctc ataatgccat aaatcactgg tgcgatagta
    35221 agcatgtacc attttctgtc gatggcttca ggaaaatttt ccataagaaa gttgtggata
    35281 ataccctgaa aagcaacttt ttcttcaaaa aaagtaaaca tggaatacgt cactagagta
    35341 cagacggcaa acatggtggt ataaaagttt aataaactac gtcgataacc ttgaagggca
    35401 gaacgggtct ttacaccact gttaactagc tcgctacgac gcttaccact agcaataaag
    35461 agagacaaaa agataactgt caacataatc caaattggta ggtgaaaccc agaagcaatt
    35521 tcacctgcat aagccctcat gatgaaaaag agagagatac caagaatgtc taaaacagct
    35581 ttctttttaa agatgaagga ataagagtac tggagcaaaa caaaaaccag ggccatgata
    35641 gcgaaaggca gatttacagt gtgagcaatt atgagacctg aaaaaaataa gacaaaaact
    35701 agtactatgg caaccataac cggcatgtca ccacgagcga tcggacggct ctttttaaag
    35761 ggatgttggc gatccttatc tacgtcaaga atgtcgttaa ctagataaga agaagacgaa
    35821 agaagacaaa atgatatgaa tccaagactg gttggtacca agttctctag atgaaaaaga
    35881 cgaccgttaa ggataatcga agcaaaaagg cttagatttt tgatccattg aaatggccta
    35941 agagattcaa ccacaacaag actttttctc aatagcacca tatctacaat aatatcagaa
    36001 atccgaacag agtgacgatg acaccgacaa cagtcagggc ctgggctggg aattcaaatc
    36061 ccgcattagg aagaattatt ctagttgggc tggggccctg aggggcagtg caacaactcg
    36121 tataactagg attagccttg caggtgtcaa cataacttgg ctgagaacag taacgattac
    36181 cgttatcagc ggagatacaa gttagcccag aagaacatgg tcgatcatta tcatcacaag
    36241 gagtataacc acagcccaga actacggtgg tgggtgtggg tgtattagtt ggaactaaag
    36301 ttccagtagg agttaatgtt ggagttaggg tgatagtcgg tgtgttagtt ggaactaaag
    36361 ttccagtagn nnnnnnnnag gagtctgggt aggggtaata gtagattttg gaatagtggg
    36421 tgtggaggtg ggaggttttt tggtgggggt ggctgttata agatcgacta aaacacaacc
    36481 agtgttatta cttccacact tataccccgc gacacagcat gttgtaccgg caccctgctt
    36541 acaccaagct ctgttttgat ttaagcatga tgcacgaatt gctggttcac atacctgtgt
    36601 tacacatgac tctccgccct gacaatgaca ttcatctgct cgacaactaa ctccaccaca
    36661 agaaataata ttgctgtttt cataatttcc acttgttgcc cccccactag actgaggaca
    36721 agcctctaaa tttagctttg tacaactatt agcataaaaa gaacatgccc cgggggacaa
    36781 atctatacat tcacactgat aaccagtgtt tttattggat gaatcttttt gacaggcttt
    36841 tgcacctaaa ctagagcacc ttgctggtaa actaccaccc aaaccagcat ctttcacctg
    36901 ttggtcagta cactgtgtct gtccaccacc tccaccaccg accgcacact tattagtccc
    36961 agggttagtg ccagcaggac agatacaacc tccgtctggt ttacaagttc cagaagtggt
    37021 ggctccagta ctagcatttt tacaggcaca ttcacctgga ccaacagctt taccgcgagt
    37081 atcttgattg agtttgacta aataaacaga cccgacaagc gatgatataa gaaaaagaac
    37141 ggtcagactc ttgtaattta gatacttagc aacgaatttc acttaataaa tttaactgat
    37201 acaaagaaaa atatcaatgc cgaagtgata actaagattg tgggcaatgt tccaccagtg
    37261 accggaggag aagacgttgt actaggataa gctaccctaa tagttggtac ttgagtgggg
    37321 atattggtgg gagacggtgt tggaatagcc gtaggaaaag aagtggcgga gggagatgat
    37381 tcataactaa gagttcctga attagaaatg agaatggaga atttttgaga aactgtctct
    37441 agtggttgtt gcaaactacc agtcttgaca gtaatagagt aactaccatc ggtgagatct
    37501 gatccataag accatttcca agagccagaa gaatcagaca tagtttcgtt actagcagag
    37561 gaagatggcg aaagttcgac aaaaatctta gtgtttgcag gggcaatgcc gaaaaaatct
    37621 ggctttgaag aagaaactac ttccccttct ttaggattta aaatactaag ggtcttaact
    37681 gccaaaacgg gcgatacgct agcaatctgg cctgcagcag aagttggaat aggagtggta
    37741 gctgctaatg aaagattgtt ttgtccgatg atgatgtctg ggacaggatt gttgttggcg
    37801 gtagtattaa caacttgagt agtaacccca tcttcggaga tgacaactat atcttcctcg
    37861 acagccgcgg tcggtggaac aaaccaatct gtcttcgatt cattaaaact attagccaga
    37921 gagattgacc agttgccgtc agccgtaaca aaagaagaca atggggctgc tccgggaata
    37981 tttaaataga caattgagcc agaagctggt ttcgtatctg ctttataaac cttgccccaa
    38041 gccaagtttg aggtgggcag agaagaagtt ggtcgagagg cggtacgaaa accatagtta
    38101 ctatgagtaa aattctgacc accactaatt atggagtaga cataactagt agttaccttt
    38161 agatttttgg cttgaaaata atgtgtgcgg gaaggatctt taatactagc caggttttga
    38221 ttattaaccc gaagtgaggt agagcaaata tcactagtag taaaagaaat atcaaccgaa
    38281 gtgtgggtta tgttggtgac ttggggatta atcggtgaac aatctccact agccttgggg
    38341 ctccagacag ttctaatttg agtcaagtaa acactaacag ctagaaacaa aactaaaaac
    38401 aaaagaccga ctatagtagg gattcttttt tctttcttca ttgatctaag tatttcctac
    38461 cttcaataat ctctagggtt gatgtatcta aatttggatc aatgggttta attagagtgc
    38521 tctgtttcac ggaagttata ggtttcatca gattatcata gctcttttca actaaaaaaa
    38581 agaagatggc aataaatgtt agaacgctga acaataaaac atagttggat aatctatttt
    38641 tctgcataat ccgatgatgg ccaatagtaa aatttgacag agaacctagt ttttaatttg
    38701 tgaccaggat ccacttctgc gctcgaggag gactctgatg ctgggatatt ttcttttgga
    38761 agttggccaa agtttatcag cttaatctct gtagctcgtt gattgccgat aatatcggaa
    38821 atcaatctag ttgaagacat aaaatcatca tcaatatcga gagagatcgt gtatggctga
    38881 acatcgggca tagttgaccc ctgagaatca gataagttaa aagtaatctt atcgatattg
    38941 ataccaagca gttcaccagt cttttgaact tgataagcag cttgataata gctggggcgg
    39001 tcaggaagac tgttatttat cacatgatat ctttcttgaa cttcagaaaa tgattcttga
    39061 gcttgaataa cgtcattaat tttggaacga agttcttttt ttaagacttt ctttgattca
    39121 atgtcaccca agagagacga tattgtaagg gcggtgggac ggattgcgaa aaacataaaa
    39181 aaagaaatga gagcaaatgt agaagcgatc tccattctct cgataaactt ttggttttct
    39241 ctttgttttt ttaagaaatc aaaaagaggc ttaacgcggg aaatttggga gttgagtagg
    39301 ttactcattt tttgtcagca gcaaaggtta acgacaagtt gacatcatat tgattacttt
    39361 ttggtttttt ctctatagtt tggatgtcaa ctgtagcaat atcaggatta actaaaaggt
    39421 tggtgattag gtcaacaata gctgactcct gacgagtaaa cagactgacc ctagcgttga
    39481 cagttttgtc cttcgggttt tgttcaagtg aaagctgttc gaaaattacc tccggaggga
    39541 cactgctggc caaagacatg agcctgtccg gatagttggg ctgattatcg tagagcgtcc
    39601 ggactagctt taacctacgt tgaaggttta aataatcgac ttcaaattct tgggtacttt
    39661 caagaatagc tttttgctgt ctaacggatt ctgacaggtc tgaatttgtt ctatcgagcc
    39721 aaaaacgaga caagaacgca gacactacta tcaactctgt gaagacgata acaaaacgac
    39781 cggctgtggt ggcccacttg accactcgag ctgaaaatga gttagcattt tcttcatcgg
    39841 gcaagaatga catttctctc cgtgcagaca tatacaaaca gtatatagac ttttgcggat
    39901 aatttaaaca agggctacgc tatctgaaga taaaaccctt tagtcgacgg aaatatttaa
    39961 tgtagtcgta gtactcatca atcttgagaa atcgagaaag tgctagaaaa gaagaaactc
    40021 caataagtac cgaaatagat gttaggaata ttaaactcat tgtcttacta gtatccaaga
    40081 cgaataggtc aagaacctgt aagctggccc aagaaactat tcccataccc agagaagcca
    40141 aggcaatttt tgaataatta aaataagctt gacgccaacc ctgaccatcg acaatggtaa
    40201 taaacatgta aaaaagtccg atacattgga caacattgcc aagagatata ctgatggcta
    40261 aaccaactat tcctagatcg gtataggtaa tgaaaaagaa actggagaca acattaacta
    40321 aaagtgagaa gaatgaaacc tggagaggtg tcttggtgtt gtgaagagca taaaaggagc
    40381 ggacaagaat ctggattata gcctgagaga ttatagctgg agtcaaatag gctaaagtct
    40441 tggctgtaag taaagtggca gaccagggaa actccttagc accaaaagca atacggatta
    40501 aggctacgcg atgaattaaa ataagagtcg taatcggtat ggcaataaag agactttgag
    40561 ccaatacttt ccgaacggtg ttacgaaata gatcaagttc gtttctggca atgttcttgg
    40621 agagaatcgg taaagaagcc tgtccgatag ttgtgccaaa aatacgacta ggcaaggaca
    40681 ttaactgaac cgccaagtta agcagagaga gggaaccggc agctaaggta ctagcaaaga
    40741 agagtgaaac agtgttttca atttctccaa gacctataga tatagtacga ggaatcatca
    40801 gacgaacgat ttccttgaca ccgtcgagac ttggatcaat ctgaagagaa taacgaaagc
    40861 ctaaagactt ggcaagaggg atttgaataa ctagatgaaa catagcccca acgactgcac
    40921 caataactac cccataaatg ccgaaagaag gtgccaatag aagaatactt gcaataatag
    40981 aaatattgta aacaatgggt gaaagtgaag gtataatgaa tacttgatga acctggagca
    41041 tgcctgtgag aaagttacta atcaaaaaaa agatttgagc cacaagcaga atcctggtca
    41101 ggttagccat aagagatatt tgctcgagac tgaagcccgg ggcaaccatg gctgacaacg
    41161 gatgggcaaa aattaggaca aaaaatgata tagaaacaaa ggcaattaat aagaggttaa
    41221 tgacagtaga agcaatagaa taagccttag cttcatcctt atggagataa ctactgaaaa
    41281 caggtataaa tgaagcagag agagcgccgc taacaagtag tttgaaaatt aaatcaggga
    41341 tacggaaagc agcattataa gcgtcgagtt cgacagcaca acaactataa aattgggcgt
    41401 aaagaaaacg gcttcgcaga aaacctagta gagccgatag gccgaaagtg accgccaaaa
    41461 caatactggc tgagaatatt gtattttgtt ttttgctacc gagataaagt agtttttgag
    41521 acaagttcat atagttgtat ttggtttgag tataagttag aatagtgact atgccaataa
    41581 gtagatcatc aaaaaaatct ttgcgaaaat ccataacaaa tcgtaaagcc aacttgtctt
    41641 ttaaaaataa atttaaagaa atggtaaaga gttttttctc taaaccagat aaagaagcta
    41701 tggaattagc tcaatcagca attgacaaag cagtaaaaaa gggagtaatt cacaaaaaca
    41761 aagcatctag gatgaaatct caacttagta agagagttgg aggcgaggct gtagttgcca
    41821 aaaaagtgaa gaaagtaact aaaaaagttg tcaagaaatt gtctaagaaa actgccaagg
    41881 ataagatgtc aaaataatta ggtatactta cttaattatt tagatatttt taaatgaata
    41941 aaaagtttta cgttacgact cctatttact atataaacga tatccctcat ataggtcata
    42001 cctgtacgac gattgctgcg gatattttgg ccagaatgca tcgacaactg ggtgatgacg
    42061 tctttttcct taccggaaca gatgaacatg ggcaaaaagt ggctgaatgt gcccaaaaag
    42121 aaggactaga cgtccaagta tattgtgaca agatagcccc aacgtttata aatgcctgga
    42181 aagaacttaa tatcagtaat gactttttca tcaggacaac agatccgaga cacgaaaaag
    42241 tcgtccaaga actgttgcaa aagatatatg ataaaggtga tgtgtacaaa gcaaagtacg
    42301 aaggttggta ctgtattggt tgtgaaaagt ttctaactga gacagatctg gtagaaaaac
    42361 attgcccatt acatccacca gagaaaactg ttcaaaagtc agaagagaat tggtttttta
    42421 aattatcaaa atatgctccg caattaattg agattctgga aaagaaaaac tatatctttc
    42481 ccggaggtaa aaggaacgag gtggtggcaa aacttaagtc gggagtaaac gacatttcct
    42541 tatcaagaga gaatgttagc tggggaatac ctctaccttg ggataagacg cagacctctt
    42601 atgtttggtt tgatgctttg attaattact atagtgcaac tcaatttcta gaagacaagg
    42661 caggtttttg gccggcagat ttgcatttac tgggtaaaga aatattgtgg tttcatacag
    42721 tgatctggca ggctatgttg ttgtcggccg aaatttcgat accagagaaa acatacaccc
    42781 acagctttta tatggtggat ggacaaaaaa tgtctaaatc gcttggtaat gtaataagcc
    42841 caaaacaatt gacagagctt tttggagtag atggggcaag atatttaatc gccagatcat
    42901 ttccgtctga aaatgattct gatgtaggaa ttgaaagatt taaagaaagg tataatgctg
    42961 acttggctaa caacttaggt aatttggtgt caagagtgag taaattggca gataaattac
    43021 gaattacgaa ttacgaatta cgagttgatg ataaatttca aaagctagtc gatgaattaa
    43081 aatttgatga agctattagt tggatatttg aagaatatat cgacaaatct aacaacagat
    43141 taaatgaagt agctccatgg aaattggaag tagcagatcc aaaaagaatt gaagtattaa
    43201 cggaatgttc tcaaaatatc agaaaagcag catattactt gaagccaatt atgcccaata
    43261 catgcgagaa aattgagaaa tgttttgaag gagaaattaa agcgccagaa agcggactat
    43321 ttccgaggat cggatgaagt atctttccga gcaagagtat catttactct atattcagcg
    43381 accatttcct ctattgatct gtacttcgtc acacgctgac ccttatggac ggttacaggg
    43441 tcaagtaact taatcttccc ttctgaacca ggagtagaaa cacgggacga aagtctagat
    43501 gccctttcag tatctatatc tggtctctct gtccttattt gggctaatag tttatcgagt
    43561 gacataatac atattatata cgtaatatta taagatttca attattcaac cagtgcgatg
    43621 cttaggtcga tagtttagac gtttactaag tatatcgacc gccatacctt ctacaaactc
    43681 aacatcgcct ggactaggtt tgggagtaga ttgttcaaag gaatctaact gtcttctgat
    43741 taaactgagt gctcgtggta atgactcttg ttgattgata ctgctgatac tcagtacagt
    43801 ctcaactaaa aggtttgatg gcatcaacct attctagcaa actctggttg aaaataggat
    43861 aaacctagcg aagggtccat ccaccgtcga cgacgataac ttgtccagtg atgtagtcag
    43921 cgttatttga agccaagaat atgactgatt gggcaatatc ttcaggtaca ccgaatctct
    43981 ttagagggat ggctccgata gtcccctgct tcatctcatc tgtcatagca ccagttgcac
    44041 cgggagactc aatggctccg ggagcaacag cattaacatt tatttttcta ctagctaatt
    44101 ctagagctag agtcctggtt agaccattta ctccaccttt tgaagcgcag taatgagtca
    44161 agccttcaaa tccaacaagc gaggcaatag aagaaatatt aactattttc cccccatcag
    44221 gcataacctt agcagaagct tgggaacaga gaaaaacact ttttaaattg acatcaataa
    44281 ctttgtccca atctgattca gataagtcca taaaaggttt gaacgggaaa ataccggcat
    44341 tgttgacgag tatgtctaat ttgccaaact tagagatagt ttcagaaacg aggtggtcta
    44401 cttgatcttt ttgagaaaca tcacaactaa cagcaagggc ttttactccc aacttttcga
    44461 tttcaataac agctgattca caaccagatt gttccaaatc actaacaaca acattacatc
    44521 cttctttggc tagggccata gcaataccaa gtccaatacc ttgtttggca ccggtgacga
    44581 tagctacttt atcttttaac aacatgagta tttttaaata tttaaatttg taatgatatg
    44641 atcatagcat gatcgatacc cactcccata tatctacaga catcggtaag ctagattttg
    44701 tagtgttgtc gggaagtaat attgaagatt caaaaaataa tcttaagatg gcaaaagctg
    44761 ataagagact aaaagcagcc attggtattc acccacagga agttggcgat aattttgaag
    44821 cattggaaaa gatgatgtgt gatcaggtgg tagcggtagg agaatgtgga ttggagttta
    44881 ctgacggggt agacagagaa aaacaaatta aattatttga atcacagata gaattatcga
    44941 tgaaacacaa taaacctcta atagtccata gccgtaaggc gagtgatgag actctagaaa
    45001 tattaaaaaa atataattgt agaggagtga ttcattgtta tactgggggg attaaaagaa
    45061 tcaaaaacgt acctgaaagt ttttattttg gtattgatgg aaatttaact tatgaaagtg
    45121 gactacaaga agtagcaaag agtatcccta aagacagact agtacttgaa actgactccc
    45181 cctttctaac cccaatacct cataggggtg aaattaatag accagaatgg gtagaatttg
    45241 tctaccaaga agtggctagg atatgggaaa tgagctttga agaagcggaa aagataatcg
    45301 ataataatgc cagaacttta tttcaaatag tctgagataa tagcttcgag ctctttgata
    45361 gaaggatact tgtcgataag agcgacttca ttgtcgatta aaagaatagt attaccggta
    45421 agacctcgag cataagcttt atttagatcc ttactatatt cagggtcatt gtcaatctgt
    45481 agacgctgac aaacagactc aatatcagca aataattttt taccttgagg tagacctcga
    45541 tcaaatagtt gtattctcat aaactattct accattgtca tattttccaa aagacaggcg
    45601 atggtcatag gaccgactcc accgaaagtt ggggtaatat agctagaaag tggtgctact
    45661 tgctcaaaat ctacatcacc attggcaacg tcgacaacga tagcctctgg ttttatatac
    45721 tgggctgtga ctaagttttt aactccggtg gcggaaacta atagatctgc tttttggcag
    45781 acctctggca aacttttagt tttgtcgtta cacaaagtga cagtgacacg ccttttctct
    45841 aaagcttttt tgagaggaat accaataaga ggactgtcgt taattattac cgcattttta
    45901 cctgataagc taatatgata ttcatcaaga agacgaataa tccctttaac tacggctggc
    45961 aaaaatagag aattttcagc tagaccatca acgtctttgt ttctagaaat agcattgaga
    46021 atttttgacc tattaagatg tggcggcaat ggcagttgga caaagaaacc ggtaacatca
    46081 acgtcattat ttagattgtt gattaaggta agtatgtctg gagtcggggt aatggttggt
    46141 aaatgatgga ctacacagtt cagacctatt tgccggcatt tctctttttt taaagaaacg
    46201 tatttgagac tagcagggtc gtcaccaact aagatgatat ggagtgaacc agtaggtcta
    46261 atggaggcaa ggattttttc ggaaagggaa cgaccgtcta aaagaatcat gattgggtta
    46321 aaatttcagc gccggtgtcg gtaactaata tagtatgttc aaactgggcg gaaagtgaac
    46381 catcacgagt acggacagtc caaccgtctt gcgggtcgat agtgacgtgc caatcagtac
    46441 cagcattaac catcggctcg atagtgaaag tcatacctgg cttgagggta atgtcgccct
    46501 ccggattggt gttgtggaat acgaaaggat cctcgtgaaa ttttaagcca acaccatgac
    46561 cgccaagatc gcgaacagga gcgtaaccaa aagggattag atacttttcg ataactttac
    46621 caacacaatc atttagtgat ttaccaggac gaatagaatt tatggctatg tcaagagaat
    46681 ttttggtacg aacaatgaga tccttagctg attggctaat attaccaaca gggtaagtgg
    46741 ctgaggtgtc tccataataa ccgtcaagaa tagtggtgac gtcgatattg ataatatcgc
    46801 cttcttttag aacatctttg gttgagggta taccatggca gacaacatta ttgattgaag
    46861 tgcaaatact tttagggaaa ccaccatagt taaggggggc agcaatagct ccgtggccag
    46921 aaatgaattt aacagccagt tcattcaatt cttcagtatt aattcccggc ttaacaaaag
    46981 ttgacagata gtctagggtc tgggcagcaa gacgagatga tttacgaatt ccatctattt
    47041 gacttgcgtt tttgatgatg accacagctg attatagcaa atacttgaca gagtccaaca
    47101 actcctacta tactggtagt agatatgaaa ccattaacca taaactcact cccctacgct
    47161 tatgatgcct tggaaccaat aatctccaaa cagattatgg aactacatca tgataaacac
    47221 tatgctgctt atgtgaataa ttttaattta gcactagaac agggaaaaat tagtagcttt
    47281 aattataatg gtgcggtaat gcatgattta ttctggaaca acatgagagc gcctatagat
    47341 aataacttac cacctgaggg aatgtttcct gattttgaaa agttcaaagc agaatttagt
    47401 gctgctgcag taacagttga agggtctggt tgggcagtat tatggaaaac tgaaaatggg
    47461 ctaatgatcg gccagttaga aaaacacaat ttacttggtc taaatggggc tgtgcctgta
    47521 cttgctttag atgtctggga acatgcctat tatcttgatt atttaaacga taggaaaggc
    47581 tatattgaga aatggtggtc tgtggtaaac tgggacgatg ttaaaaaaag aatttaaaaa
    47641 agaaacttct tggactcaag taggaaaatg gtataacggg attgtgggtg agaaaggtca
    47701 cttttaccac caggaggtaa ttattcctca cgtattaaga ctattaaacc taaatagaac
    47761 ttcgaggttg ctagatttgg gttgtggaca aggaatattg gctagagcta taccctcgga
    47821 ggtaaattat ttaggaattg atttagcagc taatttggta gatgaagcca ggaaacttga
    47881 tcaaaatcgt aaccatagtt atgcggtggc tgatgtaact aaagatttac ccataaaatt
    47941 ttctggattt agtcaaatag caattatctt ggcactacaa aatattaaac acccatttaa
    48001 ggtaattaag aactgtgcga cccacttaaa taagggagga aagctagtga ttgttttgaa
    48061 ccatccaagt tttaggatcc caaaacatag tgattggttg gtagataaaa atacaatgac
    48121 tcagtataga aaaaatgata tttatatgtc ccctttggag attccgatag atagtagtcc
    48181 gtttgacaaa aatgacaatg ttaaaacttg gtcgtttcat taccctctat cagcttatag
    48241 cgagatgctg tttgataatg gttttgtaat tgaaaagatt gaagaatggg tatcgcctaa
    48301 aaagtcagaa ggtggaatgg cagaagttga ggataaagcc cgcaaagaat tccccctatt
    48361 tatggcaata gtggcaaaaa agatttaaaa cctgcatatt agtgtgttac cattttatga
    48421 tgaagaaaca aaataaagac acttttactt ttaataaatt ccttaagttt cagctgagtt
    48481 atgaagtctt tggaggcata ttcctaataa tattagtagt ggtcatagcc ttatttagtt
    48541 agttgtgaca gatgcagagt gaagaaaaaa cgatttagtg tagtaaaatt gccctaatga
    48601 cggaatttca agtattagtt gagcaattga cagaggaagg atttttggca gaagtgattt
    48661 acactagtca atcaaaaaag aggaagagga agggctttgt tgagcttggc acaagaacag
    48721 atgtaccaga aggggaaaat gtatctattg gtatagcaaa agatagggag aatcacgact
    48781 ttaatatatt aactacgatt tttagatcgc atggattgtt gtggataaac gagagaagag
    48841 tcgactcaga gaaagtattt gaatatcctg tgggagatgt gccacaaata agggtgtctg
    48901 tattaaccag ttagctgctt gttgaaggtg acgcagattt gacggacatc aacaagtgag
    48961 aagccaggat gactgatacc ttcttttata ccattgatta tttctggtgg attgtgtccg
    49021 tcgacagaaa tagcatgaac gtctggattg acttgtttta ataaaggtac tggatcgatt
    49081 ggaatattta tcgaacctaa tggtgtagac tttgtcttgg agcctttggg gctagtggga
    49141 ctagcttggc cagtagttaa actataaata tgattattag ccacaacaac cgtaatatca
    49201 atttctgaac gagcataaga taaaagatga ttaagacctt ctccataaat accaccatct
    49261 cctccaacaa caactacagt cagttctgga ttggctaatt taacgccgat agcagtagga
    49321 atactgcgac catgaagggc atgaatagca taggtgctga aaaaatcagc catattaccc
    49381 atacaaccga tatcatagac aacaactatt ttttctctag gtacattaag ctgaaccaat
    49441 gattgttcga cacccagaaa catcaaatag tcataacatc caggacacca agtggggaca
    49501 atgtttgatt taaggttgtc tgtcatagtt gttttattaa ctcttctgga aagaaaggtc
    49561 taccatcata ttttaaaatt gttttagtag ggttgaaatc aaattgtgat ttcaaaagtg
    49621 atgttaattg actagtggca ctattttcaa taacaattac ttcagtaaat ggagcgatga
    49681 gtaatttaat gttcttatca attggccaga gagttttgat ctgtaagaaa gcgtaatcag
    49741 gattattcaa aagacgcaat gtctcgagaa tagtagaaga tggtgacccc caactgacaa
    49801 ttaacttctt agcggtatct tgaccataaa aagtgggttt ggggacaacg ttaagaattt
    49861 cttgtagttg agactgtctt ttctcaactg attttttgat tggctcacta tcctctactg
    49921 aaaaaccatg ctcatcatgt tcgtaactat tggccatata ttcttgaccg ggagtgccgg
    49981 gcaaagattt ggtggactta acaatatcaa cgtctttttt ggacaaatcc gactgagagg
    50041 caccggactc agcaataaat ttatcagaga ctaatatgat agggatattg aattgagcac
    50101 ctaaattaag ggcagtacca gccatctcga atgattcagc ttgattacct ggagcgataa
    50161 ctattttgtt aaattcaccg tgtccggcat taacagcaaa aagaagatca ccttgaccag
    50221 tccaagtagg taaaccagta gctggaccag ggcgagagac taaataaaag agtgtaccga
    50281 gctcagcagc accacagaaa ctgacagttt cagtcataag ggcaaatcca cctccactag
    50341 taccagt
//
LOCUS       DDWP01000089            4921 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_40725, whole genome shotgun sequence.
ACCESSION   DDWP01000089 DDWP01000000
VERSION     DDWP01000089.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_40725"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1637 a          753 c         1115 g         1416 t
ORIGIN      
        1 gatggagctt atggactttt taatattaat gacgagaaga aaaaatatct atttgtagcg
       61 acaggaacag gaattgcacc gtttcattcg atagtaaaat catatccaaa attggactat
      121 aaactgttac atggaataag gactaaggat gaagaatatg ataaaaaaga ttataaaaat
      181 tacattagct gcgtctctag tgatggacag agagtgactg attatttaga gaaaaatcca
      241 attaggacag acactgtctg ttatctctgt ggaaacagta atatgattaa tgatgtctac
      301 gatattctta gggagaaagg aatttccggt tcaaatatcc attcagaatc ttttttttaa
      361 atgaaagtag ctttttctta cccaccaatc gttaacaagt taggtcaaaa ggcgatggtg
      421 tcgcaaaatc gcaatgtcca attttttaaa acaccaactt atctactgcc agtagtccac
      481 gcccaagcag ctacatggct aagagaccta aaatatgaag ttctgtggga tgacggtaat
      541 tcacaactga aaagctacga acaatggttt aaagatctgg ttaaatggaa gccagaggta
      601 gtggtgttag aaagtactac cccggtgatg aatttctatt ggaaaacgat tgatgaacta
      661 cgggcaaaat taccaaagac tatttttata atgactggtt atcactcgat gagaaagccg
      721 gaggaaacgc tcgagaaatc agcgacggat gtcataatta agagcaatca tgttgacttt
      781 gctttagtaa aattgttgcc acagattaaa aagagcaaaa gttggcgcaa aacagttgag
      841 ctagaaggat tgacgattag agtagataaa aaaactattc gtgatactgg agcatttcga
      901 caaattgagc caattggaca agcaccttta gttgatcgta aattagtgaa atggaagaac
      961 tacgcttatg aaaatggaaa ttttttacag actccgggga catacgcgac ttcagtaatt
     1021 cgggattgta tgtttggtaa atgtactttc tgccgataca atggaccagg aatgactttt
     1081 tccaaaatgc cagtacaaaa gagcttggat gaatatgaaa tgttgattaa aaaatataaa
     1141 gttaaagaaa tatttgatga ttcgggggtg tggtatcgcg ggtctgatgc tagagaattt
     1201 gctcagggaa taattgatcg aggactacat aaaatggggt gttattttgg aattaatact
     1261 cgttttgaat atcttgatga ggaaacaatt aagcttctgg ctaaagcaaa ttttagattt
     1321 gtgttgattg gactggaatc gggaagtaat gaaactttaa agagattgaa taagggttat
     1381 caaaatgaat atgtaaaaca gtgtcttacc tggatgacaa aatacgggtt gcacccacat
     1441 attacagtaa tggtgggata ttattgggag acacagaaga tgcttgatga gacagtggat
     1501 tatgttaaag aaataatgct ctctgggcta tcgagaacat tgcaggtaac tctatgtaca
     1561 ccgcttgatt acacaccata tcatgttgag tgtattgata aaggagtact attgactgat
     1621 aattatgatg atcatgatat gagtaagttg attgtcaaaa caccaattcc tcatgaaaga
     1681 tattatgagg cgatacgaaa aatgtatctg tatagcttct atccacaagt gttgtggggg
     1741 catttcaaat tgatgtttaa cccccgcttt ggaccaatgg ttttactggc ttatggatgg
     1801 agggcgataa gaagggtgaa gaatcacttg tttaatttga ccgaagcaca cgctaaataa
     1861 ttaggtttga catatgtttt ggatatgtta tattagttgt ctatgaaaaa tataactcgt
     1921 ggtagtgaaa tcaaaactgg gcaaggattc gtgctacata taactagaag tgaaaatgct
     1981 ccagtagaaa gagttgaggt aaaagttgct gaattatctg gtaaaacagc tactgttaag
     2041 ggagaaacgt ttgaagcaag ttttaatgtt gatgttttgg gaatggtagg aacaagaata
     2101 aagtaggtta agaaatttcg acttgtttta tcttatcgcg gatttgaata gctaattcaa
     2161 agtttaagtc ggcggcagca gaacgcatct cggctcttag cttactaata tgttttttct
     2221 tttgagatgg agtaagagat tcgggatcga cactaagaat agatatttca attttgtctt
     2281 tggtgggatt ttcaataatc cggggtcgaa tagatttaat aatagtctcc ggagtaatac
     2341 cgtgctcttg attataagct agttgtattt ttcgacgacg gttgacctct tctgtagcct
     2401 ctttcatagc tatagaaatt tcatcggcat aaagaataac ctgaccacca acattacgtg
     2461 aagctcggcc catgatctga ataagagccg aacgagaacg gagaaaaccg gcctgaccgg
     2521 catctagaat agcaaccaaa gaaacctcag gtaaatcaag accttctctc aataaattga
     2581 taccgactaa aacatcaaaa tcaccgtttc ttaatttgtc gagaatctca gacctctcta
     2641 acgtttcaat atccgaatga aggtaggcaa ctttaaaatc tttgcttaaa aattgagcta
     2701 gatcttcaga cattctcttg gtaacagtga ctactaaagt cctttctttc ttggcgacac
     2761 gatcggcaat ttctctaact aaatcgggaa tttggttgtg actcgggcgg acagtaatcg
     2821 gagggtcgac tagaccagtt ggtctaatta tttgttcaat aatgttattt tttgaatcat
     2881 caatttcaaa tttggaagga gtggcggaga cataaatagc cttggggata cgagaataaa
     2941 attcatcgaa ttttaaagga cggttgtcat aagcactggg cagacgaaaa ccatattcaa
     3001 ctaagttggt tttccgagct agatcaccac cactcatacc accaatctgg ggaacagtga
     3061 cgtgagattc gtcgataact gttaaaaagt tatcgcccca aagatggtga aaataatcaa
     3121 taagagtgta gggagggtcg ccaacttgac ggtcgggctc gaaataggtg gaatagtttt
     3181 caataccgtt aacatagcca acttcattga gcatttctaa atcgtaatcg acacgctgtt
     3241 ctagtcgatg agcttcgagc aatttatttt ctttcttaaa tgcctcaaca cgatcggcac
     3301 agtctgattt tattttggcg tatatttctt taaggtcgga agatgaggtg acaaactgtt
     3361 tggcaggaaa aaggcgataa gagtctaatt taaattcagt actagaaaat gggttacgtt
     3421 gggtaatact ttttatcttg ccaagttcgt caaattccag agagataagc caatcggcat
     3481 atgaaggcca gatttcaaaa ctatcaccac ggatgcgaaa ctgagaacgc ttgaattcta
     3541 tttcggagcg agtgtaataa agacttacca aggcttcaga gaggctacga cgagtccaag
     3601 tgtcaccctg atttagatca agggtcttgt tgccaaattg agtaggatcg ccgatattgt
     3661 agatacaaga gacagaagca ataacgatat tgtcagaccc agaaaataaa ttactagtgg
     3721 cttcaagacg aagcttatca ataagatcgt tgatctcgac ttctttggcg atataggtgt
     3781 cgctattggg aagataagcc tcgggctggt aataatcgta ataagacaca aagtaggaga
     3841 ctttattgtt ggggaataat tctttaaatt cttgatagag ctggccagct aaggttttgt
     3901 tgtgagaaat gatgagggta ggcagctgag tcttggctat gacattggca atagtaaaag
     3961 ttttaccaga accagtgaca cccaagagag tttgtttgcc aataccggca ttaagattgg
     4021 tgacaagttt gtcgatagct tggggctgat cccctgccgg agacataggt gaaacaagtt
     4081 caaaagacat tatatattgt aacaagattc ggttttaatt tgggtatttt attttgttaa
     4141 taatgagagt aaatttatta tgtgggtgat gtagttattc aagtaagagt aattaaagga
     4201 cgacactttc gtaaggaaat ggatatatct tggcccgatg gagccagaac agaattaaca
     4261 cctgttaagg gagtactaca tggagttgat gggaaggatt atacggtgaa gagaggagat
     4321 gagcatatga agaattgtag aaagataatg gaaacacagg gatattcgtt taggagacgt
     4381 tagtgctaac taaagtagct acggtggcaa attatggatt aaaagcagtg gatgtagagg
     4441 tcgaagttaa tgtagctgag agaggtttcc cggggtttgg gattgtgggc atgagtggta
     4501 aatcaacaga ggaagccaaa gaaagaataa agacggcatt aattaattct gatatcgaat
     4561 ttcccacagc caaaattacg gttaacttgg caccagctga tttacccaaa gaaggatcgg
     4621 cttatgatct accaattgct ataggaatat taggatcaat gtcagaagtt gaaataccaa
     4681 aaaataagac atactttttt ggagaattat ctttggatgg aagtttacgc cacactaaag
     4741 gagtattttt actagcggta ctggccaaag agcagggggt aaaagaaata tttgtcccga
     4801 gattgtgtgc caacgaagct tgtgtggttg agggaataaa agtttatcca gtagataatc
     4861 taagaagtat tattcgacat ctaaaaaaag accgattaat tgaacctttg gcattaatca
     4921 a
//
LOCUS       DDWP01000090            6201 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_26504, whole genome shotgun sequence.
ACCESSION   DDWP01000090 DDWP01000000
VERSION     DDWP01000090.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_26504"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2053 a         1116 c         1388 g         1644 t
ORIGIN      
        1 aaattggcag cagaaaaata tcagccaaaa agtctgatgg cttcgggcgg agtgttggct
       61 aatgcagaac tgagaaaaag gttgagaaaa atagctagag aattaaaact accgatatat
      121 atgccagcaa aaaaaatact caatactgat aatgcaggga tgatcggaat tgctggatat
      181 tttatgacac tacgaaaaga gtgggcagat ccggaaacaa tagacaggga tcctcgatta
      241 aatttctgag gaaatttttt tgtctagttc acgcattgtt tccataaatt tggtctcgag
      301 atcaatgttg tattttttgg ctaaaaccaa aacacaccaa agacaatctg ataattcatg
      361 tcccaacttg taatccacgt cgtcaatacg gcggacacct tccttagcca tgactaactt
      421 cattagatca cctacatcac ccacaaaacc ctccatgatt tcttgattag tccacacgtg
      481 accattcttg ctttcaagct ggtgatattt ttgacgaatt tcaattgctt tatcggagat
      541 tgttttaaag tccatggata attaaattat taattagttt tagtaccgcg gcggtagacg
      601 gcggcccaag ggacataagt gctagtaaag gttttctgat gaagtacttc accatttttg
      661 gtcactttcc aatcaaaaga tgtcttgagt ccagagatgg ctctttcgtc ctgaatcact
      721 ttgcccgcgg gtaaagtcgg gtcgtcaata taaatcgtag gtggaggtgg agcggcgccc
      781 cattgacgat aattagtgat ctcagtttta cgaccgtctg atgtgccata tatctcgtat
      841 tctaaggttt tatctgtacc attataatta ctctgaatta gtaaatgatg gccagtgtca
      901 ttaacaaact ttaaatcagg actggggata aaaatggtag cgtcgtaacc aggaggagaa
      961 tcttcttcgt aataaccaac acgataggca tggtgtcggc gctcagtgat atctaggcca
     1021 gcatcgagca tggctcggaa aaatgtagtg gagacttggc aaataccacc accaacatca
     1081 agctcggtct tgccagcacg aatgatataa gccattttgt aaccattttc taaagagaca
     1141 tcgccaaggg atttgacaaa agaaaaagtc tcccctgggg caacaagaat tcggttgatg
     1201 acttcggcac cacgctgaac attcatatta cgaatggcac tgctgtgctt aaaggttgat
     1261 ttacctttac ccaaaagctc aacaatacca agatcattca catctgagtt tttagttttt
     1321 ggatcggtgg taatgatggg gaatcctgat attaattttg gctctgaaga tgcgataagt
     1381 tcatttagct tggtacagat tagagaagag acctgagatt cgtcaagaat ataaccaggg
     1441 gtggctggct taaattctaa gacttggccg ttttcaaact tgaaaacagc ttcaatggga
     1501 tcttttttta gactgccttt taaaccacca acatagtcat taatattgtc gttgttacaa
     1561 acgctagtaa aatcgatcca agaaactagg gtcttgtcat cgatggtgac gacttgatcg
     1621 ggaacggtaa actcgagaga tttgccgagt aatttggtgg cgttagattt ggcgagagat
     1681 atctctgaat cactgggcaa ctgaccgatc tgagcggcag gtatctccag aggagcagaa
     1741 aaactgtagc cttgaagagt agagataatt ctgtttttta actcaagtat ttcggtcctt
     1801 tgtcctaact ggccatcttt gaggacgata gacttggtct tggaatcgag tgaaagctcg
     1861 gaaggaacaa agggactatc aatttggctg gagagattaa tcaattggtt gtccagtgat
     1921 actgggtcgt agtcaatgtc tagataaaat tctttaggtt ggaaaaaggc ggaaaaatag
     1981 ttgacaagac cggaacgaat acgtctaaaa agaagattag aggctgtctt ttcgtagttt
     2041 acagaagcgg agatagaggc tagattaagg gtaaaagaac gatcttggta cgtaagagga
     2101 attaactcgg gtaatgggaa atcttgggag agattagaaa taatttgtga tttggaaaga
     2161 agggaatagt cttggcctaa gacactagta ttacgaccag tcttaccaaa accagcgatt
     2221 aaaggaaata aaccaacaaa agcaataata aagatgaaaa ctagaaaata gatgaagatt
     2281 tttcggttca ttttcggtcc atggcttgat tagctaactc gtcagcagcc gaatttaatt
     2341 cacgagggat ttcgtgaaat ttgtaactta tatttatagt tgataaaaga gaaacggctt
     2401 gtcggtaaag aggaatgata gtcggagctt taaccttgta tttaccttga agctgtcgaa
     2461 ccataagttg ggagtcggaa taaaaattga gggaagaaat atcgtaattg actaaattgt
     2521 ctttgaccca aattaaagca tccaaaaggg cagagtattc ggcttcattg ttggtcttga
     2581 ccccaatgaa tttactgatt tgactgatga ttttttttga ttcgtcataa acaacgacgc
     2641 caaagccaga gggaccaggg ttaccgcgag atcccccgtc agtgtaaata ttcaaaacca
     2701 tagagaattg taacaaaaca ctatgaagga agtaatcact aacaagttta atccctattc
     2761 aatatgtaac tttgtgatat tatgtgctca atatgacaga aaaaacacaa agatacgcag
     2821 gtggattaag acagactttt gctaaagttg gacaacaagc tctaactcca ttagaggttt
     2881 ggaaagagaa ggtaactgca ttagcacaaa gaggagagag ggtgaaaact tctccatctg
     2941 tacctggtga gaatggttta agacagactt ttgtgaaagt tggtcaaaaa taaaggtata
     3001 actttatttt tttatttttt cccaaggata ctcagcatgg ccgaagtggc cataacgagc
     3061 tgtcggagtg tagattggtc gacgtaagtc aaagaattcg ttaatacctt tgggtgataa
     3121 attaagtaag ctccaagcaa aatcttcgat aactttttgt tctgaagtgc cagtgccaaa
     3181 agtctcaact gatttaccaa ctggttctga ttgaccgata gcataagcca actggacttc
     3241 acaacgaatg gcgaggccag cagcaacaat gtttttggca atataacgag cagcataagc
     3301 agcactgcga tcaaccttgg aagtgtcttt accagaaaaa cagccaccgc caattctacc
     3361 cataccacca taagtatcga caacaatttt tctaccagta acacctgtgt ctgaagtcgg
     3421 cccaccaatc tcccatttac cggtaccatt tagaatcatt ttgtcttcgt caattctaaa
     3481 gttgtagtcc gctagaaccg gaacaataac attgtcgtaa aaaaaatgag agtaatcagc
     3541 aggagcaact tgataattta ctggcttagc aaggacaact ttttcaatag cgactggagt
     3601 gtggccatcg taacgaacag tgacttgaga cttaccatcg ggacgtatcc agcggtgaac
     3661 tcgttgtaat tcgtccattt tacggcataa gctatgagcc atagaaatag gtaacggcat
     3721 taactcggga gtctctttac aggcataacc aaacatcatt ccttgatccc ctgcaccacc
     3781 agtatcaact ccctgagcaa tgtcgggaga ttgctgatga atataaacat caatatcgga
     3841 cttgtcggta aactctaaga caggatcaag ataatctaat tgagcaattt tttggcgagc
     3901 aatggcagca aaatctaatt taccatttag agtaacttca ccggctaaaa caactttgtt
     3961 gatagtaact agagtctcca caccaacacg ggcctgggga tcgagggtgt aagcggcatc
     4021 gagaatagca tcactaatct ggtcacaaac tttatcggga tgaccttcac agacggattc
     4081 tgaagagaac tgaggataat tcattttttc ctttgttaat aattaaaggc gtgttacagt
     4141 gggcaccgtg atccttcgac tagcttagga ttcggttgtc aacctaaaaa ggtcttctaa
     4201 acacaaggga atattacaaa gaaaaaactg acttttcaat cacttactct aacaacataa
     4261 gggaagccat gagggcaacg ccaagaagga tgaagagtac ttgaactaaa gaagaccact
     4321 tgtcagattg gtgacgatga atctcgggaa tcaggtcgga agaggctaaa taaataaacc
     4381 ctccagcagt aacaggtaac atatagacag aaaaatcttc aacgagattt cccaaaagga
     4441 aggtaaaaag tacaccaact atcgaggagg cagccgtgaa caggttgagt ttaagggcct
     4501 gaaatagtgt cttaccttga tgaacgtata tagcaaagtc ccctatttct tgaggaattt
     4561 cgtgtagcaa gactgcgaca gtagtaataa ctccaagtga tggactaacc ataaaactag
     4621 aggcaataat gacaccatca ataaaattat ggagaacttc accggcgaga ctgttggcaa
     4681 ctacatgctc agagtgatga ccatcgtcgt cttcctggga gcaacagtcg ggattgtgac
     4741 aatggcgcca acgaacaatt ttttctaaga taaaaaaaat aattattcca gaaattgtta
     4801 aaaaaggtac aactagacct tcaccaattg tctcgctagc ttcgggtaaa agatggataa
     4861 aagcatcacc gaaaagagta ccgacagcca aactaaccaa ataaacaata taacgagaaa
     4921 gattggccac gcgaccaagc aaataataaa tgataataat ggagatgaaa ctaacggaaa
     4981 cgacagaaag aagggaatat atcagagtta acatgtggat agtttaacat ggggcaaatt
     5041 ttgatatgat tgattgatgg cattagaaga tattaaatca ccgttaccac cgagaaggtt
     5101 ttcgggccta tacgaaacag agacggaaga tcaaaagtct gaaagtctta aagtcgaaag
     5161 tacacaaaaa gatgaaagtg agccggtggt gccattaaat gcaccgctga ttagcggtaa
     5221 gatagaaaaa aaagtagatc acaaaagtta tcttgtatat gctgggattg gagtggctgt
     5281 ggtggcaata ttaggactag tatttggagt gataattcca aaagtgatgg gtggttcgaa
     5341 ggtggaagaa gtaaatctaa cctattgggg gttgtgggaa gatagctcaa taatgggagg
     5401 cataatcgca gaatatgaag cggctaaccc gggagtaaaa atcaactata tcaccaacca
     5461 gaaagtaaat taccgaacga gattacaaag ccgactggaa cagggtggtg atacttcagc
     5521 cccagatata tttagacttc atagcagttg gctaccaatg atgcaggata acttggccaa
     5581 agttccaagt gatgtagcaa caggaattgg attggataca gattattttg atgtatacaa
     5641 aagaagtatc aaggatgggg ctaattattt aggaatacca ttaatgtatg acggattggc
     5701 ccttttttat aataaagatt tgattgaatc tggtggagtt actttaccta agtcgtggtg
     5761 ggaccttgaa gtggcggcta acaagctaac agttaaagat agtgctggcc agatcactgt
     5821 ggctggagta gctctggggt tgaccgataa tgtagatcac tggagtgatg tggttgggct
     5881 aatgcttaaa caaggcgggg taaagatact tgacccggga gaagatagta caaaaaagat
     5941 gaaagatgtg ttgacttact acacgttatt tcggactaaa catgaagtgt gggacgagac
     6001 attacctaat tcaactgaaa tgtttgctaa cggcaaactg gccttctatt ttgcacccag
     6061 ctggagggct tttaatattg aggatatgaa agtgccggag ttgagatatg gaattgccac
     6121 agtaccgcag ttaccgacat taaatgatgt gcccgtagat caaattaata acgaagctaa
     6181 tttaactaat attcactggt c
//
LOCUS       DDWP01000091            3184 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_36488, whole genome shotgun sequence.
ACCESSION   DDWP01000091 DDWP01000000
VERSION     DDWP01000091.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_36488"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT          784 a          765 c          502 g         1133 t
ORIGIN      
        1 ggtttcaaaa aaggtgtgaa gaccaaggag atttgttatt gtcacactcc gcctcgctgg
       61 ctttatggtt tcaaaactag cgttggtttt caaaaatatt ggatagtcaa attgtacgcc
      121 gccattgtcg gtcactttat gcgtctttac gattttcagc aagctcaaaa agttgatact
      181 tttcttgcca attccaagaa tgtttctaat cgtattaaaa aattttatcg ccgtgattct
      241 cagatagtct atcctccagt ctctttaccc tcagttccaa aagtcaaaaa aggggacttc
      301 tatctcatta tctctcgtat tgttggtgcc aaaggtcttg atttggcagt taagactgct
      361 ctcaaattaa atttgaaatt aaaaattgtt ggcgagccag ccggttatta caccgagtac
      421 cagcaattac aaaaattagc tggtaaaaaa gttgaatttt tgggtcgagt ttccgacgaa
      481 gaactagtca agctttatgc cagtgctaag gcattcttag ctttgtctgt tgacgaagat
      541 tttggtatta ctcccgttga atccatgctt tgtggtacgc cggtaattgc ttttagaggt
      601 ggtggttatc ttgagtcggt tgttgaaggc aaaacaggta ttttttttga cgaacctaca
      661 gtcgcatccc tttcttccgc catcaaaaaa tttgagaatt caaagttcaa atcctccgac
      721 tgcatctccc aagccaaaaa atttagcaaa gaagttttta tcagtaaaat caagaaaata
      781 gtttcaggct ctattcttta gctccttcct ccctttcttg ggcttgcagc aaccatctcg
      841 gcttgacttc ttctttaaaa cttaccttcg gaaatttttc ttttgcaaca cctctagcta
      901 ctggtacatc ttcatcgcaa gctcttatct gtatttggta gtcaggacgt ttatgttgac
      961 caaccaacac cactgtcggt ttttggtcac aaccattacg tacacacctt agtattgatt
     1021 ttggtataac ttccattgcc caggattata ttacggttct tgaaaaaggc aactatcttt
     1081 ctatctttat ccacttttct gcgtctagtg ccgctttaca tccatctcca gcagcagcaa
     1141 tagcttgttt gtagttttta ttcatagttg tgcagtcacc ggccgcaaat attccctcat
     1201 aagacgtcat tgttccgtac tcttgattct ttcctgttac cacatgtcct tcttcatctc
     1261 tctctaccat ctcagccacg aattctgttg ctggattatg accaatagcc ataaacaacc
     1321 catctactgt caaaatactt tcttcatttg tcttattatt cttaattttt atatctttga
     1381 gactttcaga cttttgactt tcaactgtcg agtttatctc gacaacttca ctattccaca
     1441 ccatttctat ttttggatta ttcagtactc tcttttgttc aactacgctt gcccttaagc
     1501 tatcccttcg atgaattaga tataccttgg ttgcaaattt agttaaaaaa tcagcatctt
     1561 cacaagccgt atctccgcca cccactactg ctaccacctt atctctaaaa aacattccgt
     1621 cacaagtcgc acaaccagat actccttttc caatcaattc attttctttt tcaattccta
     1681 gccattttgc ttttgctcca gtcgccacaa ttaccgcttt ggtttgtaat tctgtcccat
     1741 cctctagcgt tacgctaaag ctctttggct ctaacgctct aacgctcttt acatttcctt
     1801 gtcttatttc tcctcccaag ttagttactt gagcttgcat ttccatcatc aacttcactc
     1861 caccggttcc gtttgcatat cccggccaat tatctactgt cgttgtcatc atcaattgtc
     1921 cccccggctg gttacccgcc agaactatgg ttttcatatc cgccctgatg ttatatatcg
     1981 ccgccgtcaa accagcaggt ccacttccaa ttattactgt gtcgtaaata ttgttattca
     2041 tagatctatt atacttgata ctttttatca attcgtaatt cgcgcagtaa aacaagtcct
     2101 cctgggggac aattcgtaat ttataattat tctagtgatc actcgtaccg tcgcttcaat
     2161 taattttgtc ttccagcacg ttgcccacaa aggggagttt ttcttttggc tttttattcg
     2221 ccttctttct gctgccttac cccttattac catctaccag ttctcccgtc ttatcgaaat
     2281 ggtcgagcaa aaagctgttt tttctgactt attttattat ctccttctca tttttgctgt
     2341 tcgcattctc gataattttc tccgcctccg ttccaccact aggctagatt atcttattag
     2401 caatatttcc tttgatattc acaatttctt tttaatcgac tttaaacctg ccaccaagga
     2461 agatcgtaat gtttctatcc aggccattcg taacttcgcc gatgccacag tcgccacttt
     2521 aacagtcttc aaacagccag gtatcgacag tctcgtttct gttcttttta ttccagtcgc
     2581 tttgtatttt attgatttca aatctttcgt cctaattatt gcctatattt ccatctacgc
     2641 ccttataaac ttttatgcca gccaacgcta caaagagctc cgcgatctcc agaacaccaa
     2701 aaccgaagcc tactttgcca aacttcaaga cagtaatgat gtcgagctag aacaatcaac
     2761 ctttactcga cactttcttc gtcttaccaa ctggaatttt cttgagtggt ttgctcttca
     2821 aaataccgcc gttgtctttt actcgatctt tgttctttat caagtattcc tagtttctac
     2881 cggggctggc cacctctctg gtttgatctt agttgttggt tatgtcactc agactcagac
     2941 attcttaaac tcttttacgg atatctggtc tggactagag gacatgtctg tagctctcaa
     3001 acatcttgct aagaaccgtt ctgtttcagt tattgatttg gatgatttaa cctaaagtgc
     3061 cagagttgcc agaggtagaa gttgtcaaaa tctttcttga agacaagctt cttggtagta
     3121 ccatcactga tcttgagatc ctcaataaaa aatcttttat cggcgatccg tcagaggtta
     3181 ttgg
//
LOCUS       DDWP01000092            7537 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_35950, whole genome shotgun sequence.
ACCESSION   DDWP01000092 DDWP01000000
VERSION     DDWP01000092.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_35950"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2451 a         1385 c         1387 g         2314 t
ORIGIN      
        1 tacagcaagt acagccagga ttatcaataa ccggtgagat agtcttcgac ttaccagatg
       61 atgcaactga ccttaaatta ttagtaaagg gaagtatgtt cgggactgaa aaacaaatat
      121 ctctgacaaa ataataagaa gatattaaaa attaaaccct ctgagaaatc ggagggtttt
      181 gggtttaggg cataaacata tagttatatt cgtcgttagg accaaagtag tgtcctttat
      241 ttagtagagt atcggccaat tcccaagcgg tcatgttttc cgaaatccgg taactggcaa
      301 caatatcaat cgagccattt ttaccaacca acaatgatcc tgggtttcct tgagtagtat
      361 cttttgtatt ttccaaagca tacattaatg ctttttcatt cctgatgaaa ccataatatt
      421 gaaggttgct tacgattgcc tctagtgtag agcctttacc gggtcgaaaa tctacagtct
      481 cactttgttt ttgcctttct aaatcacttt gagtaaatct aagtttttca acaaaataat
      541 cctcggcttt tttagttgag ttccaacttt ttcctggagc tttaaagtct tcgttgggaa
      601 ttttgttgaa gtttaaataa gttgccacag aaattccgac aataattaag attatgacta
      661 caaaatgttt cttcactatt ggaattataa gccaaaatct ggcaattgat atttacaaca
      721 atcattgttc attacctgta tatgcttcgt aaccttctct ccacaaaata acgacgaaga
      781 ataatcccgc gagtggatct acccaccata aaccaaagaa gtaattaact cccaatgcga
      841 ttagaaggac agcggacatc caagcgctgg catacgactc catagaatct gataataaac
      901 tttgtgaatt taatagtttt cctgtctggt gtttgagatg agccattata ggcataacaa
      961 ttattgacaa gatggcgata cctattccta gtaaactcgg ttcaattact tcatgaaaaa
     1021 taagtttttt tatcgcttca tataacacat aggctcccaa gataaagaaa gtatgggcga
     1081 ctagtttctg agctttccta tctatgatat gctcctgttg ttttgattgg catttgtgtt
     1141 tgaaccgcca aatcatcact atacccgata gcatttcgat aacactatcc aaaccaaacc
     1201 caattaaggc gatgctccct gtaattacac ctaatccaat tgatataatt tcttctgcaa
     1261 gatgataccc aacattcatg taagacagca tcagagctcg tttttgtaag ttatttttca
     1321 ttattttaag taccaatctt atctaatatt accatttgac aaatataagc atatattgat
     1381 atatttatga ttatgacatt aaattcatct caattgcaac tggaggcaaa attatttaaa
     1441 ggatttggtg atccgtcacg gttggcaata atagagtcag tctctaagaa accgttgact
     1501 gttactgaaa tagttgatgc tacagggcta tctcaaccaa atgtgtcaat gcatttagcc
     1561 tgtcttcttg actgtggatt ggtacttaac agaaaagacg gacgcaattc gttttatgag
     1621 ctatcaggat cagaggtaaa gaaagtaaca agtttggctc aaaaaattgt cagccaacac
     1681 tccaaacaat tatttgaatg tacaaggtat taaatattaa aaaaacatat gtgtaataca
     1741 acagaagaaa ataactcagt tgactacaaa tggtatacct atccccaaat gcgtaatgcc
     1801 cttatctcgg gtggaattat tggtattaca cttgtacttg ccaagatcgg aatcttacct
     1861 gagtttatct ccatcatcat gtacgttgtg gcagccggga ttggtggttt ctattggatg
     1921 agagaaggcc ttgaagaatt tatccatgaa aaggaaatcg gaatcgagat acttatgttt
     1981 gctgctgcta tcggatccgg aattctaggt ttatgggagg aggcagcgct tctggttttt
     2041 atctatggtg cagctgaagg tttggaaggt tatacgtttg ccaaaaccag agcctcaatc
     2101 cggaagcttt tagatttagc accaaaggaa gcccgggtag tacgacacgg gatagaaact
     2161 atcattctcg ccacagaact ggaactgaat gatattttca ttgtccgtcc aagtcagtct
     2221 ataccaactg atggggtaat tattgagggc gaatcgagtg tcaatgaagc gccagtaact
     2281 ggagagtcat tgcctgttga aaaactgaaa ggaatgaaag tttacgcggc aactattaat
     2341 caggaaggtc tgcttaaagt taaagtcact gccgtatttg ccaataacac actatcaaaa
     2401 atggtccata tggtcgaaga agcccaagaa aagaagggta agtcacaagt atttattgaa
     2461 aaatttggta aacgctattc tccaatcgtg ctggtatctg gattagctct catgattatt
     2521 cccccgcttt ttggattatc atttccattt tgggcggagc gagctattgt ctttttagtg
     2581 gccgctgctc catgtgcttt agttatgtct acaccagtcg ccatctctgc cggaattgga
     2641 gtagctggta aaaatggtgt tctcattaaa ggtggacgac acttggagaa tctcgggaaa
     2701 atcaaagtag ttgccttcga caaaaccggt accttaactt taggcagacc gatagttact
     2761 gatattgtgt caatcaatgg agatgataaa gatattctga agttggcagc caatattgaa
     2821 cagcactctg aacacccact agctaaggca gtagtggcaa aagccaagga attaaatatt
     2881 aaattaaggg atgtgaccga ctttaaatca ctaacggggg ccggagcgat agctacaatt
     2941 gaaggaagaa aaattaaggt tggtaaggaa gagttgtttg aaagtatcga ccaagaactt
     3001 catatcaagt cagacaaatt tcttaaagaa gggaaaaccg taatttacat cggaactgac
     3061 aagaaaatgc ttggggcaat cgcaattcgt gatgaaatcc gtcctaatgt agtccaagcc
     3121 atcaaggact tacataacct aggggtaaaa gtgtttatgt taaccggaga taatgaaatt
     3181 acagctcaag ccattgctaa ggagattgga attcatgagg taaaagcggg tctaaaacct
     3241 gaggataaat tgaccattat caatgagtta aagaaaaaat atttgggtgt cgctatggta
     3301 ggagacggaa ttaatgatac tccagcctta gcagctgctc atgtaggaat agctatgggt
     3361 gccgtgggaa ctgatgctgc gattgaagca gcagatgtgg ctcttatggg agatgacatt
     3421 accaaaattg cctacgctat cgaattgggt caaaaatcaa gatctataag tcaacaaaat
     3481 attgtgttct cattattgca attggcggtg ttaattccat cggctctgat aggtattatg
     3541 accatcacca ttgctgtggt cgttcatgag accggagaat tactggcaat agcaaatgga
     3601 ttgcgggtag cgaaaactaa ataatataag aaagaagagt tgatttagac taaaccaatc
     3661 aacttatcgt tatgatttga tttaaacaac ctccatccga aaagtgtcac cataaatgcc
     3721 tcagttataa ctgttaccca agctccggca atcattccaa aacgtggaat tagatataca
     3781 ttgagtcctg tatttattaa caaggctact attgctatta gtaataaata tttttgatta
     3841 cccttggcta ttaatctaaa tccaagaaca tgaccaaaat aggcaaaaac tattgcaaat
     3901 gataagacct ttaaaacagg tacagctgcc aaatattctt taccagctaa aaggttaatc
     3961 attaatggag caaaaaaata tacgagcggt actattccaa gaagcatgag tccagctaca
     4021 cataaagttt ttttagtaat atttttatgc tcatttttgg acattgccgg aaatacagaa
     4081 gaaaccaaaa aatatgcagg tataactaag ttgctatata ttttataagc tagtccatat
     4141 tgagcaacat taatttctcc ccaaaaatgt ctgattagag ttgcgtcaat agctcgatca
     4201 taagctgtaa aaattaacat atatagacct attggccaac tttcatataa tatttttttg
     4261 atcaaaagcc agtcgatttg ttttgtaaac ttaaaattat aatcggaaaa gtggaaatta
     4321 attaatttcc aacctataca caggctaata attctactaa gcaagtatcc actaaaaacc
     4381 aaacctagat tcaaatattt tccaaaaatt aagataaaaa taaaaaaacc agcaggataa
     4441 acaatatcaa taattgtttt taggtcaagc ctagatccgc tttgaaaaat aatttcctcg
     4501 tcacccgcaa cactggtaag ccaagacatg gataaggcca ctaaagacca aaacctaaat
     4561 ccctcaaaac cgggatacaa aaagaccatc aataacccaa aaaagaaagc cactcctgtc
     4621 attatttttc ttaaaagagc aatatttatc catattttct ttctactttc cacatctttt
     4681 gcagccgata tttcccgaac cccattagta cgcgtcccaa aatcagctac tgaatcgact
     4741 agcagaaata ttgacgaaac gaaagttaac aaaccataat cattcaatcc caactttcgg
     4801 gttagccatg cggttgttcc taaactaata aagatagtaa ttatcttgcc gaaagtttgg
     4861 atagaagtat atttgatcaa ctgttttaac acgtatttat tttatcaacg aattattaat
     4921 aatactaaaa cgtaaaagct tcatttatat atacttatgt gtaaatctat agtaataacg
     4981 aatggcagtg gaggttgcag atatattttt aagtgctaca ctatgtagta tgaagcacgt
     5041 aagtacaaaa atgctaggtt cgctacaagc aaaaataatg gatatcatct ggaaagcaga
     5101 acgtccatta aagccagctg aagttctgga acaactcggt gagggttacg cctacaccac
     5161 agttatgaca attctaaaga gactttctga aagaaaaata ctaactagga aaatggaagg
     5221 taaagtttat ctatattcgc cagtatcatg tcgaaaagat tttgtggaaa caaaccttaa
     5281 agatattttt ggtgatttgg taaattccta cggaaaaact gccatcgcca attttgttga
     5341 tgtcgtcaaa aataacaagg aagatatgac tttattaagg aaatatctcg aatctaacaa
     5401 atgaactcca aaagattaaa tctaatctca aatctttatt tttatttgat ttccttatcg
     5461 gggctttttt acacagcctt attaattatt gcttcggaaa aaattattcc agctttttta
     5521 atgcaaataa tttttttatt agatgcattg aagtttgata atagtattat cggattggta
     5581 acttccaaat tgtttttgtt taatgtcatc cccggattaa ttttattagg gcttgttatc
     5641 ggttacgcca agactctaat taaatcaatc aagggtattt atgctgtacg agcaataaca
     5701 agcaagctcg acgttgttgg tgctacccaa gaattcttca aatttaagtc aaaagaaata
     5761 tctattttta ctttaggatt ttttaggccc aagattttta tatcatcagc tatttttaag
     5821 actcacaacc aggaagaaat tgatgccatg atcgaacacg aaataaacca cagaaacaac
     5881 ctacatcccc tgaaaatatt tatctctaat tttgtcagat caattcttcc aattattccc
     5941 ggtaaaaatt ggctgattga taactatcta acgttggttg aggtgtcttc tgatcaattc
     6001 tctgaggaca aaataaacaa taaattgcca cttatttcag ctttattaaa gtttcaaagc
     6061 caaagttttg aaccagggat tagttacttt aatagccaaa gtgaaagaat taaaatatta
     6121 gtgggacaaa agaagcaact tttaagaatg cctctaactt attattcttt tgtcttagta
     6181 gcaattttat ccagtgccat gttcttgaaa aactcaagta ttttctttga atgtcagcac
     6241 ttacttaaat gtgtcgagat gctagttact tcaaataagc agtcacttat tgcgccaata
     6301 tatgccaata gtagcgtcat ttcgcctaca gaccactgtg agcaaactag tctattgaca
     6361 tctcactaga cacatactac actgtgtagt atgttaaaac aaaaaccaac aatcaggact
     6421 tccacctact atgtgcaggg gatgcattgc gcatcatgtg aaatactgat tgaaaagaaa
     6481 attattaggg aagactcggt agaaatggtt gatgtatcac tctctaaggg cacggtaacc
     6541 gttgaatatc agactgacaa aaaaataaca ccggagtatc taaataatat ttttgctgaa
     6601 gatggttata gtttttctcc gaaccctttc aagaaatcga cacaaattgc caccgatagt
     6661 cactgtgcta tgcccgacga agaaccgtcg aatccatata cttcattgtt tatcgccgcc
     6721 tcttttatca tcggttatat ttttctttct aaaacgggat tatcttcctt aatttcagtt
     6781 aacgcccaat cgtctctccc aatttttttc ttatttggtc ttttagctgg aatttccagt
     6841 tgtgccgctt tggtgggtgg aataatttta tctttgagca aacagtggct ttcgtcgtat
     6901 agtgactctg attcgacgct gaataaagct cagcctcatt ttctcttcaa tattggtcgg
     6961 gttgtcggat acgctggctt tggagcgatt ttaggaatgg caggtaactt ttttagatta
     7021 tctccagtag tttccgccgg actagttatc ggagtttcac taattatggt tttacttgga
     7081 ctgcaaatgg tcggcgtcaa agcccttcgc aattttcaaa tcagattacc taaatcaatt
     7141 accggaaaag tggctgatga atcgaacttt aaaggtcgat tcgccccact tttgatgggt
     7201 gccttaactt tctttttacc ttgtggtttt accattaccg ctcaagcttt agcactagcc
     7261 tctggaagtc ctttccaagg agcaatgatc atggggctgt ttgccctggg gacagtgccg
     7321 ggcttgttag ctatcggttt gagtagtgtt aaatttcata gcaacccaaa gtcatccaag
     7381 cagttttcga tgattgccgg gttattagta ttatttttcg ctgcttttaa tatcaactca
     7441 caattatcag ttctgggggt ttccaatgtt aatgatttaa tctctgctcc tagtgcccgg
     7501 accacccaag cgacaggtga actgccgcca atggtta
//
LOCUS       DDWP01000093           29436 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_258, whole genome shotgun sequence.
ACCESSION   DDWP01000093 DDWP01000000
VERSION     DDWP01000093.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 29436)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 29436)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29436
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_258"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         9088 a         4087 c         6740 g         9521 t
ORIGIN      
        1 gggcaaccac ttggaacttt gagggtactt cctttaaagt gtcaaattaa tctagattag
       61 tgattggtat tctgaataat cgcgtatata gtcatttgga tcgtactcat gagaggcaaa
      121 acacaataag atagcgtcgt cggagtattt gtattgtgtg gcccaaacca tatgtggtat
      181 taatattcct atactcgggc tgtctaattt gatttcttct ctaatctttc cattgtcaag
      241 tacgattgaa atagatccgc ggagacagat gagaagttgg tcacaaatct tgtgggcatg
      301 ctccccccgg acatcttttg taggaacgtc ataaacgata aaatatctct ttggtttaaa
      361 cggcatatct tttctaaact cacttgcaat tagacttcct ctcaaatctt gatgttttgg
      421 aaagtcgtac agtttggctc ctttaattgt tagtttggtt ctatcttgtg agcttttttg
      481 gtcttgtttt actgtcttgt tagagtttgc gtaaccggaa attctggctg gattgccaat
      541 tacaattgca tttgctggta cagagtgtgt aacaacagat cccgcaccga tcattgcgtt
      601 ttgtccaata gttatgcctg ggagaatggt tgaatttgca ccaactgatg cacctctttc
      661 aactttagta actaagaatt tttcaggata tttcttgctt cttggaaatt tatcgttggt
      721 gaatgttacg tttggtccaa taaaaacatc atcttcaata atcgttccgt cccacaattg
      781 aacaccacat tttatcgtta cattattacc tatcgttaca tcattttcaa taaaagtttg
      841 gtcacatatg ttacagttat ctcctatctg ggcatttggt aagatatggg caaatgccca
      901 aattcgagtg tttttaccaa tcttcttgct ttcacaaatt ccgtttgggt gtataaaata
      961 attcatatgc ttaattgtat aagattttgt ccataataat tgtgttcggt ctatgttttg
     1021 tattttcaaa cattctccac acataaagac caattatgcc aaatgaaaat aagttgatac
     1081 tgctaaaaaa tgagactatt aggagggtgg cagtataacc tggtacggta attgcgcctg
     1141 ttaatttaga aattatgaca attaaagata atgctgcaga cattagtatg ccaattagtc
     1201 caaatttaat taatatcttg atgggtaggt ctgaaaatga gaagattgag tcagtcatgt
     1261 atttaacttt tttgttaaaa ctccatgcac ttttaccata tttgcgcttc actctactgt
     1321 aatcaacata ttctcttttg aatcctatcc aaaacaatag tccaattagt gaactattgc
     1381 tctctctgag acttattaac acatctctaa ctaatttgtc gcaaccaaag atatctactc
     1441 caccaggtgg gacgtcgttt tgaatgtatt ttttatagat tttccagaac atattcgaat
     1501 atagattatt tagatagcta tctcttcttt cgattctttt accaataacg atattggaat
     1561 tatgtttact gagttttttg aacatcttaa ttactaaaga gggtggttct tgcatatcgg
     1621 cagccatgac tgaaaaatag gttccggttg cgttttccaa tcctgtttta atggccgcga
     1681 aagatccaaa atttctagag tgctgaatta gttgtgattt aaatcggtgt ttgtttaatt
     1741 gttgctttaa aatttcaaat tcgttttctg ggtttccgtc tattacaaat acaatttcta
     1801 gattgtgatt aagcgataca tttaactttt ctagtgagtc cagcaattct ggtataaact
     1861 gagcatttct gtaaattggt attataagtg aatacatttt atcttgtttt ccagctatta
     1921 catatcttta ccactttatt agcttcctct gttgtgatta atgagtttat cggtagtgaa
     1981 agttcttgtt taactatttt tttggcgtta tctagtgaac cggatattat gtgaggaata
     2041 ttgtgtagtg ctgtttggtc tgtgattagg ataggataat gaattccact ttcgatttcg
     2101 ttctgtttta agtattcttt aagtacatct cggtgttccg aaaaaactgg aaataaatga
     2161 tatacgggtt ttgagttttt tggaatttgg agtaggttga tttcactgtt tctaattccg
     2221 ttgatgtaaa ttgaggctat ttgtcgtcta tgttcggttt gtttttttaa ttttggtaga
     2281 aaaacatctt tcattataat tgcctgtaat tcatctagac ggctattcat tcccacgatg
     2341 gaatggttgt acttgctgct ttgaccgtag tctctaatgg tattagaaat ttcgtgaagg
     2401 ttgctgtctg tggtaagtag cgcgccacca tcaccaaaac atccgaggtt tttggttgga
     2461 taaaaactgg tggccgttat ctgtcctact gaacccacaa agccactttc atcttctgca
     2521 agtactgctt gagcacaatc ttcaacaatt tttagatcaa agtcttcttt taacttttta
     2581 agtttttcaa gattaatggc ttgtccatat aaatgaactg gaataaaaaa ttttacgttg
     2641 gggttgtttg taaagaagtc tcgggccaaa tttaggtcaa ttaagccgtt ttcatctgta
     2701 tcaacaaaag ctggtatgcc gccgcatcga agtattgcaa gggtggtagc aaatgcacta
     2761 agtggtgttg tgagtacgat atctcctggt tttataccta gtgccctcaa tccgatttcg
     2821 atggcgtcta gtccgcttgc gcaaccagtg cagtatttat tgttagggta aaacttagtt
     2881 agttcttgtt caaagtcctt gacaccttga ccaaggatgt accagccact tttaccaaat
     2941 ttttttactg aatcaataac ctctttttct atttgagtcc aaagtaactg aaaatcattt
     3001 agtttgatca tattattcat ttttgtaagt tgcttaatta tattattata ccctgtcttg
     3061 attggaggtg aatatcttcc tgtaatctgg atatttatta aaaaagaaat tttctatctt
     3121 gtcaatctgt tgtggtgtat attcagcttt tttttcaaag atgtgaatct tggacccatc
     3181 ttctaggctt tgcgtaactg atgtaactat tttgtaattg agtccaaact cttgttgttt
     3241 aatgatattg ttaagaaagt atccaacaac taattgtctc tctggattaa ggtgtgacca
     3301 gtttggagaa acaacaatga tatatctaga cttaaatagt tcatggggta atccatctat
     3361 tttgtctacg tgggagtgtg ggatgttgag gtgtttgcag agatctttat tgtgatgagt
     3421 aaaaaaacaa tagctgttga ctgttgacga gttaaatagt cttgaactgg ctagtgtgta
     3481 gattttgtcg tgtggctgta tatctattgt ttcgagttta gagaatagct ctgtaatggt
     3541 ggatagatca tttctgtagt aggggaaatt gggtgatgtt gtgaggacct tgttaactat
     3601 cggtggaaac tctataggta taaatgtttt tatgaaattt aatgatgcta gaaagcatat
     3661 aattagggtg aaagctatag agagaatccg tttgtttttt agggaagtga gaattgtgaa
     3721 gatggagtag ctttgaaaat ataatatagg aagtaagatt agataatagt tgtgttgtcc
     3781 aaaattctga gctgtaaaga aaattataaa tgtaaagatt agactgatga ggctaaacag
     3841 agatatgaag cttgattgct tgtgttttag ccaactcaat gtttgaaatg taattccgag
     3901 aagaaataag atcgtagaaa atgaacccat aaatgtgaat aaacgaaaga tctcttttag
     3961 taagttgaag tctggcctgt aagcgtcatg catgtcgcta taatttcgaa ataacgataa
     4021 aaacagttca ttaaagagca gatagagaat tattaatgag atgccaccaa tttggaatat
     4081 tctgaataga ggtcttatgg ttttttttat cgattttgag gagtttatta ttgcaatgat
     4141 actggtggta aataaaaatg aaataacact aaacgcgtac catcgcctaa agataaatgg
     4201 aagaactagt accatggata ttaaaattac cgtgtgtgtt tgtggttttg tgagaatttt
     4261 tgtccaatat atatatatag caaggagcat tgggaacaaa ccgacaatat cgatatatcc
     4321 tttaataact ggagcccaga ttaaggttga taagacaact atgaaattca cggttgtgag
     4381 tattagggta tcgttgggca ctttatgcaa tcccattaaa cttttcttga ttattgatac
     4441 caataagata atgcttggaa ttgcgtaaag tattgcaatg ctcgtaatat atagatgtct
     4501 tgactttatg ctgtcaccaa atattagcgc aatgattagt cctggggtga agttgtagtc
     4561 atatctaccg atactattcc aggttaataa aagtccttct gagatatttt ctctaataat
     4621 tgtacctagc ctggtcgtga attcccagta tgccccaaag tcccaataat atataaattg
     4681 ttccctatct atgtaatatt gtaagaatat gaggataaat atgaaaagcg aggcaagggt
     4741 aagtagttgg tgaactttat tgcctgtatt atctttcatt acttctatgc gatttgatta
     4801 tagatacttt ctagttcaat ccgattatca tatattgatt taacttttgg tattttggtt
     4861 ttaaagaggt taattaatgt tggattgttg tatatgtgta gtattttttg tttgagtgat
     4921 ttagagtctc cagctttgaa gtgaagacca ttcttttggt ttgtaacttt ttcggccatg
     4981 ccacctatgt tggaacagat tactggtatc tttgccataa atgcttcctg tatgactaat
     5041 ggtgaattct cccaccaaat tgatggaact accaaacaat catatagact taggatgttt
     5101 ggcaactctg tttgtgagta tggtccatgg aatttgacgt tgtcaagttg ttttattttc
     5161 tctaagatat tttgattata ttcttgatca tcttgcattt ttccgtaaat gtctaaactt
     5221 atgttgtagt tagttaagac ttccattgct tctaaaagta tttggagtcc tttaaaataa
     5281 tttatttgtc cgatgtagat aagttttagt tgtccgcttt gttgtctatt attgctgtct
     5341 gctttgtgtt caaatatatg ttgtccgttt tctgatacaa ggaatttgtt tgatttaatt
     5401 cctaagtcta cgtatcttga tttgatgaaa tttgaaggtc cagtgaatag atcgatgtat
     5461 gaaaagtaga gtttaatatt tttacgacga tcagatatta ttttttttgg gatatacgga
     5521 aaacactttg aacactcttt gagtgatgat ttgttgcaga gtgtgttgtt ttgccatttt
     5581 gtttttatca tttgtccaga atttggacat atgcccatat attcgtgggt agttaagact
     5641 atttttgtgt ttggtagtgt ctcttttgct attttaaacc agctgagtga taaatggaga
     5701 taatgctcaa aatgaataat gtctggtttt gtgttgatta aaaactcttt aaagaaatcg
     5761 tcactttctt ggttagttaa gttaaaaata tcaacatgtg gcgtgtgtgt gtagaatttt
     5821 tgaggatctc tctcgtcgct ccatatcaaa tttgtccaga atttattgta gtttgtacgg
     5881 gaaagaagta ttggaaaaaa agtatcactt tttttgaatt cattgaatag gttgtttatg
     5941 tacacctcaa ctcccccata tgattctggg ggtagtccgt gtgtgatgaa cagtaccgtt
     6001 ttagttttag acatattgag gataagtttt aattaagaat ttagaccact tgctttggta
     6061 atagtcatga ttatgttgat atatcaaact tcttttcttg cttggtgtct tgacagttag
     6121 cccctcataa tgaataagaa cactttcgta agaaaaaaat atcctcttgt tgtctttaac
     6181 tattttgttg caaaaatcag aatcatcaaa accaccggct ccaagataat gatagtctaa
     6241 catgtcatgg ttaaagaagg ttgttctttt tgttgctagt gacgcacctg taaccatagg
     6301 tactgtactg gatgttatct tgggaaattt aatattaaaa aatttgaagt agtgataatt
     6361 gtatatcttt ttgtctattt ttcgaaatgg atacatcccg gcgtgttgga caagtttttt
     6421 gtctgggtaa atcaaaaaac tacctgaaac ttcaatatcg ttgcccttta gtggtttaat
     6481 gagtgcagat aaccaggaat tgtttatgac ctccgtatca tcatttaata aatgtagata
     6541 ttcaccttta gatatctctg ccagcttatt aattgatgcc acgaagccta gattgttgct
     6601 tccagaaatt attgtaacgt gaagtttttt tagtaagttt ttgttctctt tagcccaatc
     6661 ttcaactgtt tttttggaat ctggtttgtc gttacaaatt agtatttcat aggtcattgt
     6721 atcggggtgt ttgttaattg agttcaacaa aacatctaaa tgacctgtag atccgtatgt
     6781 aggaattaat atacttacct tcggagagtc tgtatgattt tgaacaatat attcgtatgt
     6841 ggttgtcttg agacgagtaa agaattctat ctctagatat ttaaaataaa attgatggta
     6901 aatatatttt acaggtaaaa gtatttgttt atatggtaag attttttgtc ttgtatggtt
     6961 aatcaatata ctcttaattc tatttatctt ttgccagagt ttgtatgatt ttgcattgta
     7021 tatcttacta agttcgcttt ccaggcgata ttgttcgttt agcaattgat ttcggctatc
     7081 tattaatttg gagttttctt ccttctgctt cattaaattt tggatgcttt gttgtgtgtg
     7141 tctcatatga ttatagtttt ttggctacgt cttttatatt gttgtatgtg cgccatattt
     7201 taaaaaattt tgagctctta atctcgttta gattttcttg gtggagtctg tttttttctt
     7261 caatgttttg gatctctgat tttagttttg tattttcctg gataatatta ttgcttgttg
     7321 tatctacata tcttttgtag ctttcgtaat aaattttata gattttggtg gttttatttt
     7381 ttgtattgta tttttcgtca aacttctctt tgtgtagttg gtaggtatat tctcgggtag
     7441 ttttaatggc ttgagatata tatcttgtta tccttaagtc tttctcgtct gcattatttg
     7501 aacttgatcc tcctattatc ttggaatata attttattat tttcttgcag ttatgattgg
     7561 catcaaattt tttgatggcg agatttttta ggatctctcc ttgagaatgg tcgtatctct
     7621 tgatttcttt aattaggtcg ttgacagtgt agttttttga atagtgtctg cccgaaaagt
     7681 tgttaaagtt aagcttgtca aagtttttac tagagacgac cccgtcgcct ccaagatagt
     7741 catatacaat tggtatcctt ccgcagagca ttgtttcgat aacccctctt cccagtgaga
     7801 atactatgtc agatttgttt atctcttttg gtaaatcatc ttggttgata aaaccaaatc
     7861 gtgatccttt tgctattaat tctatatttt gttgccgaca agcctctcta atattctcct
     7921 cctctttttc gcctattctg gagcttaata gaagtgccct ttgtggaact ggattaactg
     7981 tcgtggttgg gtaaaacttt gaggtgtcga ctaaattacc tatggtggta atcatatcgt
     8041 cgggaatatt gtatctattt aggttttgtc tcacctcttc acttattgct atatagtgtg
     8101 aaatatttat gttaatagga ggtggctgtt ctaagaatgg taagattccg tgggactcga
     8161 atattattgg agtatgtgga aagatatctc taatctctat tgcattgatg ttgtgatgaa
     8221 cgtgaatgat atcgaatggg atgctggaga attctttaag gtcaccggag actgtgatgt
     8281 ctaatgatga aaatttgctt aagaaattgt tgacatagtg tgaatatata taaactttat
     8341 gtccgttctg ctttaaaaat tcagctaacg tagcggtaaa tatttctgtt cctccacagc
     8401 ttagtaggtg gtgattggta agaagaatgt tcataataaa atctcttagg tcttaatttt
     8461 aacaaagtat ttcgcttttt gttgtaacga ccggtgacaa tggtgttgtc ttaaagtgtg
     8521 tttttgatgg tgtgatcatg atgagatggt gggttaactt attctatgtt taatgtaatt
     8581 tatttctttt ttgttgaaat tgtgttgtcc tggagtgccc gtcctaaaaa ctttctttaa
     8641 agtatcaggt aaatttttac cgtctttagt ttttttaatg agctttggta tttggttagt
     8701 gtcgatttcc aggtagtcca ttaaacgtgt tagttctgtt tctgtattgt tccaaaggtc
     8761 ttcatagtta attgtgtagt cgatgtattt aagatagttt gattctgctt ttgagaattt
     8821 attatataaa gctgttgctt cagaaatgtt ttttggagaa ttgctccaga actcgtttgg
     8881 ttgaatcatt gaataaatca catccaaact atttcttttg attaaaatta tttttgattg
     8941 aggaagtatt tcctttattt tatctatgta aaataagtgg gatggtgtct tctcaaccat
     9001 ttttttgttt tttggtagta aattaatttt gtgaatgatc tcttcgtccg ataagttacg
     9061 tgcgaacaga ccagtttctt ttgtgggtct gattgtttct atttcgagca ttgatggtgt
     9121 gatccctagg caatcggtgt ggttttcaag aagagagagc agccatgtag tgccagacct
     9181 tggtttacca acaatgaaaa ttatgtctct tgttcgtaaa ctgtgttttt ttacgataaa
     9241 ggaaattttt ttgtatagaa tattattttt gatcgctagc atatattttt attgctttgt
     9301 tggcatgacc atcgtgttgt attctgtgat ggttgatata tataactctg ttacaaaatt
     9361 tttcgatttg attaagattg tgggatacta atattatcgt tacgccgtta gttttgaact
     9421 cttccatttt tttaaaacat ttttcttgaa atgaggcgtc gccaactccg agtatttcat
     9481 caacaatgag aatttcgggt ttgacgtgga tggcgatact aaaggctagg cgcatataca
     9541 tacctgaaga atagtgctta attggttgat cgataaactc ttctatctct gagaaatcaa
     9601 taatgctttg gatattctcg actatttgtt tacgactgag accgaggata gcgccgttaa
     9661 gaaatatatt ttctctaccg gtaagttcgg ggtgcatgcc agcaccaagc tcgataagag
     9721 ggctgatcct accttggaca gatatagtgc cagaagtagg agacataacg ccagcgataa
     9781 gcttgaggat ggtggactta ccactaccgt tggggccgat aatgccgaca cactcccctt
     9841 ttttgatttc aaaagaaatg tctttaaaga cagtgaatct atcgactgtt ttttcaccaa
     9901 gaaaaagagc tggcaagaat tctttgaatg ttttttgatt gttcttagag aaatccttgg
     9961 aaacgttttc aaatttgatg atggtatttt tcatagttta aatattgtcg gcaaatattt
    10021 tttctcggga tttgaagtat tttaagccga tgacaagagt gaggactgat aaagccaggg
    10081 caataagaag actagagtat tttggctcaa gaccgccgag gataacttgt cggtaggcat
    10141 tgataatgac ggataaagga ttaagctgga aaataaattt atattgatcg ggaactatgt
    10201 cggcgggata aatcactggg gtgaggtaca tccaaaggat gagtataagg gagaggagat
    10261 attggatgtc gcgatagaga agattggcag cagcaaaaaa gagagataat ccgagagtaa
    10321 aaatttgttg gataaaaaag atgggaaaaa tccataatat gctaaagcta ataggaatat
    10381 ggtaataaac catgtaagca attagaacag tactggcaaa taaaaagtcg atgattttgg
    10441 caataatggt ggaaacaacg aggattgttc tgggaaaata taccttggtg ataagtgagg
    10501 ctgagccgac taaagaagca ctggctgaac cgagtgaatt ggaaaagaga gtccagggca
    10561 aaagcccgac aaaaaggaag atggaatagg gaatatgaga tgaagcattg gtgggaatac
    10621 gtagaataat tgaaaaggca aaagacataa ctaacatctg agcgagcgga ttgagaataa
    10681 cccaaaaata acctaagact gattgtttgt agcgggcttt gacttcgcgt ccaaccattt
    10741 gccagagtaa ttcgcgccat ttgacgatac tggcaatttc cttaaatagt tcttgcattg
    10801 acgatagtat aataggatta tgatcaatag tacaatatgc acgattggtg gtggaactgg
    10861 gatgccggta attaatgaag ccttattaaa agctggctac aaacatgttg attcggtggt
    10921 gactactttt gacagtggtg gtgatactgg cagaatgaga actgatgaaa gaggtaggat
    10981 actggctttc tctgattatt ggagatcact tatatctttg tggacagatg gtaaacaaaa
    11041 agctgattgg gtagaaatgt tgaggtttag agatggaaga ggacgcaact ttggtaatat
    11101 attttttcaa tttatgtcgg aaaaggcggg taatttgtcg gatgttgata atttgtttag
    11161 tcatttgact ggagcaaaaa ttaatgggag tgtggtgcca gtgtccctag tgccaactga
    11221 tgtctgtttt gaaactatta gtggggccaa gtattgtgga gaacatcatt tggacaatct
    11281 gagaatgtct tttgataaag tgtcaaaggt atggttaact ccagaagtgc ccgccaacga
    11341 agatgcttta caggcactag agagagctaa ggtaataata ctttgtccgg gaagtatgta
    11401 tgggagctta ataacaacta tattgccaaa gggagtaagt gagacgtata aaaagtctaa
    11461 agctaaaaag atactaatga ctaatttaat gtcggtggcc aatgaaaatg atggttatac
    11521 tcaagatgat tatgttaaga tatttgctcg atacttgggt gataaaaagc cagtggatca
    11581 ggtgatcatg gcggatatta ataagcttaa gggaaagtct tttgagaaga caatgcaaaa
    11641 ttatgaaatg gagcattcgt atccaattag attgtccgaa aaacaaagct acgactatga
    11701 aacattagtg atggatgtgg cgactattga taaaagtaat tttcgtttaa gacattctgt
    11761 atcgaagttg gccaaggtct ttgctacaat gaatttatgt cgcaaaggaa actaaaaaag
    11821 ttacggaaga tggaggcttt ggtggaagtg gcgatggagc aaaaatgggc ttttaagcca
    11881 ttagaggtgt ggcagacgat taaggagaat tgggtgtttc tcgccgtaat gtcgatagtg
    11941 attactctta tttacttgaa ttctttaaat ggggattttg tgtcggatga ttatgccaca
    12001 atattgcata atccagacat cgctacttgg aaatacgcat ttactagcgg agggacaact
    12061 tattttatcc atgcgatgat tgccaagatt tttggagtat ttcccctccc ctatcattta
    12121 tttaacactg tactttatat cgcgacttgt tggttggctt ttgtgtttat cgataatttg
    12181 tttggtaaga aggtagcgat ggtaacgatg attatttttg cagttcaccc aatccatgtg
    12241 gaggcggtgt cgtggatatc gggtaagggt tacttgatta tcgcttttta tacttttgtc
    12301 tcgatgttgg ctttaataaa gtattcggag acaaagaggt ggctttactt attggtggcg
    12361 attgggtctt ttatactcaa ttttttgaat gataagccac gaccgttctc attatttttg
    12421 ataattgctt tgtacctact ggtacgaaag gtggatctaa aaaataatcg gatatttaaa
    12481 atgtggcctt attttattgg cttagctatc gttggagtaa tattggcctg gccatatata
    12541 attgccagaa taggcgtggt taatggtggt tataatgctt cggagagtat attttataat
    12601 ccatttttcc agtatcctac gggagtatcg aagtatttgc aaatgttgtg gttccccact
    12661 gatctaacgc tttatcatac gatgtacact ttcccggtgt ggctcaattg ggcgatattg
    12721 ttgacttata tagtggcgac aatttatttc ttttttaaaa acagaccata cttttttgct
    12781 ctggctttta taatggcggg agtgttgcca tcgataatgc cagttaaggt tagctggttg
    12841 gtggcagaga gatatatgtt tctcgggtcg ttagggtggt gcttatttgt gggattgata
    12901 ctggcggata ttggtcgaag atataaattg ttaatgccaa tattgttagg agtggtggtg
    12961 gcttactcga gtgtccgagt ggtgatgaga aatagtgatt ggcagacaaa tcataaattg
    13021 tgggtaaata cttgccaagt gtcgccaaat tctcataatg cgtggaacaa tattggtgat
    13081 gattatgaca agcttggtca atatgaaaat tcaattaagg gatttacaca gtcgactgta
    13141 gtaaaaccaa actacgctga tgcctttcat aatagagcca atatttttta taaaattggt
    13201 agactagatt tggctcggga tagttataac acggcattgt tttattcacc agctttgtac
    13261 caaacatatt taagcttggt acaaattgac ctaacagaaa agaagttgga tctagctttg
    13321 gaacatgctc aagcggctct aaagattcag ccaaataatc cacaagcagg gtatgtgttg
    13381 gcaatagtcc tgtcgcaaag tggtcagaat gataaggcaa aactattact tgaagagata
    13441 ctgagaatgt atcctggcta cactctggct gaagatgcgc taaaacaaat gaagttattt
    13501 gaaagcggta gtggtaatag tctctgaact aaatagttta agaaaagggt aaaagagata
    13561 aatgattgct ctccaagggt gttgtctgac agaccagaag agatcgagag gatattcaaa
    13621 tatttgaggt gtgcttttaa tagtatgaaa acctgaaccg gtaagggcga gctttaggct
    13681 agtggggtta gggagataaa gatgcctggg ggcgtcgaga tgaaaataat cttgtctacc
    13741 aagacgaaag ccaagactgc tggaatttgg agtattcata ataatcagac caccagggcg
    13801 aagggagtgg tagattttat taagaactgt ctgaggtgag tcgaaatgct cgagggagtg
    13861 ccagagagta atacagtcgt aataattggc gggaagagaa cttttttgaa tgtcggaggt
    13921 aaacatttta atacctcttt tggaggcgat gcgagcagct tttttattta tctctagacc
    13981 gtgtttactg tattgtgaag gcaggtggga gagaaattct ccggggccac agccgatgtc
    14041 aagaattttt atatgattgg gaaaaccaag caaagtctcg atgtggattc ttttgagaat
    14101 aaaggaaatg tgattgagga agctgatgaa ttttgaaggt gaggggtaat aactgccttg
    14161 gtaagcttgt tggtagtatt gagaggaggt tttaaggtta agaaaataag aagtgcaatt
    14221 tttacagtaa tataaattat attgagtatt gtcaatacga attcggccat gattatttgg
    14281 agcggtaaat gaccgttgtg tgtgttgact acggcagagg cggcagtgga tcatactatt
    14341 tgaatatgga ttgttgcatt ttttggaaac gatatgacca gctccattta tctttgatta
    14401 gtttaagggc ttcttttgat atctgttgat aatgtggctg atcggtaaga agacggatga
    14461 tattgtcggc aaagttttgg aggtcaccgg gggtggactt gaccatgccg tagggatagt
    14521 aagtcttgag agctaggagg tcaaatgaga ctcctggtag gccccaggcc atagcctcgg
    14581 cagcggccat tcccccactg tcatagatgg ctggatgaac aactatttta ctgtcttgaa
    14641 agatggatgt tttttctgat ccgtctttaa agccaactaa ttttatgttt ttattaattt
    14701 tgtatttttt gataagttgt ttgacttttt cttctaactc accgttacca atcataatta
    14761 atttggcttg gggtagctgt tttaatacta agcgccagat agaaataagc tcgatgacgc
    14821 ctttttgaga gtgaaaacga ccgagataaa cggcgtcgta ttgacgagat tttttgttga
    14881 ggggctgggt tacttggtcg actccacctt ggataataaa tgctggacat ttaggaaagt
    14941 attttatatc tgggtcggaa gtgacaaaga cataatcggc aaaatgtttg accaggaaca
    15001 gagaaggaat ctgtccaaga taatatatcc aaccacggat agtttggtgg ttaacattgt
    15061 agggagaatt tttatcccag ggataagggg cgaccaaata atagccagca atccaagttg
    15121 tactggggtg gaagatttta taaagtagac cgatgataaa gtcgggataa aagtcggaaa
    15181 cggtgtagac atagtcaaat tttttgagct cttgaaaatg acgaaagaaa aataatttgg
    15241 cgataataaa acgacggatt tgattgataa atagacggag ggtatttatt tttggtaagg
    15301 gaaatgcatt gtcgatttga aaatattctt tggttaatgc ttggtggcgt ttgaacaagt
    15361 cgagagcttc agcagacccc atgatggaca cttctagata ttgttgccaa tacttaatca
    15421 ggttgatgga gatagtatct cccccacttt taccaaaacc aagattgttg ttggagataa
    15481 caattaactt ttttgctttc acttctgtta tattaactta tattatggca aaaagagtaa
    15541 ttaaagttaa tttaggtagt ggaccgacgg gaataaacgg ttggtcgaat tatgattggg
    15601 gggtgttgcc tttactttcc aaaatttatt ggataaggga tggattggtt aagctgggac
    15661 tactagataa aagttacatg gcaaaatggc cagaaattga attagttgat ataagaaaag
    15721 gaatacctca ggaagatgat agtgtaaatt atatatattg ttcacatgtg cttgaacact
    15781 ttgaaaagtc agaaacggtg actttactac atgagtgtta tcgggtgatg aagaagaatg
    15841 gggtgatgag aattgttcta ccagatcttt taaaattaat aagtagttat tctaattcag
    15901 accagttttg tcgggaattt tatggatata acaaggatga aaaaagatgg tcgaatattt
    15961 ttattcgagg acatgagtgg atgtatgata aaaaaacatt tagaaaaatt ttagttgagg
    16021 ttggttttaa aaatataaag ttactttctg gtggtaaggg gaaagtacca gatattagta
    16081 aacttgattt gaagattcat gaaaaactaa gcttttatta tgagtgtgaa aaataaaaaa
    16141 atttttgtaa ctggagccgg gggaaagatt ggatcaaaac tagttttggc tttaaggaag
    16201 tcagggtatg atgtgttgac actaaaaagg ccgggggtag atatattgga agtggctaaa
    16261 tataaaaaag aaatgaggag ttgtgattat gtttaccatt tggtggcata tcaaaatgtg
    16321 cttgataaaa aggaagatga attttatcga gttaatgtta agggaactca ggcaataatg
    16381 agagcattga aagggtcgag aattaaaaag tttttgtatg tgtcgacggt gatggtgttt
    16441 gacaaaaacg agacaaagaa tttttatgtg acaagcaaga gagaggccct aaagttagtt
    16501 aagaaaagtg atctgccttg ggtggtggtc tacccgagta ttgtggtgga tttaaaagaa
    16561 aagatggtct ggtggagaag attgttgact ggaggagtgc caggaggttt gatgtcgcgc
    16621 ttgggtgata gagagagatg gataaaattt atttggattg acgatttgat agaggagatg
    16681 gtggacctgg taaaaaatag ccgagtggga aaagaatata tattggaaaa agaaaaggtg
    16741 cgagctaagg attatatgca attgatgagg gataggatta gggcgataga acggtaggag
    16801 agatgttcta taatttattt gtggttagat ttgctgaaag attcaaaatc gagtcatcta
    16861 ggttagcagg gtggaattat tctggtaaag ggatttattt tataactatt tgtacacgat
    16921 atcagaacaa tttctttgga aagataatgg acgggaagat gatttacaaa gtggcgggtg
    16981 agatatgtgt gaattgtata aatgaaattt cgaaacattt tccagacgtt cggttgatag
    17041 agtctgtagt tatgccaaat cacgtacata ttctattcca tgtagagacg cacgatcgtg
    17101 cgtctctaca agataatcga aaaatcacgt tgataaattt gggtcataaa aaccatccgg
    17161 actattattc aagattaagc aaaaaatcta atcaggttgt ccctaacgtg attaagcaat
    17221 taaaatcgtc agtcaaaaga aaatgtaacc agcaaaagtt atattttgtt tggcaagaca
    17281 gatattatga tgaagttatt aaagacaata aaaggttaaa tacgattaaa tattatatta
    17341 agaataatat aaagaactgg cagaaagaca aattgtatcg tgcttaatag tggtgagtga
    17401 tgtaagagag attttttttg gaataatgtt ttaaggcata ggggcgacag ttatctttta
    17461 gttgatcata tatttttttg ttggtgtaaa gcttttcaat tgctttggtg aaattagaga
    17521 cagtcggggt gataaaaatg gcaactgatt tgtcggcagc ttcgatggtg ccgccaaggt
    17581 tactactaat aacaggtgta ccacaagcga ctgattcggc aatgattctc ccccaccctt
    17641 catgatattt tgatggtacg cagagtaatg atgatgattg ataatattga tttaattgtg
    17701 agtatttgac atcacccaga agggttatat ttttaagttg agggattttg gcatagtctg
    17761 ggccggtacc gataaaagtg aatttaattt gaggaaggcg ttgagctact attgataata
    17821 gcaaaacatt tttggctggt atcatgcgac cgacaaaaag aacggagaat tgatgattat
    17881 ttttttttgg ttgaaaattg ttaagatcta cccagtgata ataacgagag acatttttga
    17941 tggctaattt tttaagttga accatagaac tgtcggattg ggtgagtacc atatcagtat
    18001 ggtttaatat ttttactaaa attttagtag agagagagtt gagagggacg ttgtcatagg
    18061 tcgaatagat gctgactata tgttttttga tattgaatat tcttttgaga aagataccaa
    18121 taattgctcc attaattccg tgagaatgaa tagtatcaat tttttgaggt cggaaaagca
    18181 gtgatcgtaa taataagtag ggggtaatat aaagtaagtt taataatggt aatttttcaa
    18241 gtcggtgaaa aatattgcca ccaaaccatc taaaacgatg gacgagggaa ttgttgccta
    18301 ttttttctgt cgcttgatag ctggtcttgg ttgataatgg agaataggta aggacaatgt
    18361 tgcgatagtt ttgcttatct agttcgttaa ttaagtcggt aagatgggtc tcaacaccac
    18421 caatattggg tgagaaaaat ggagtaatgt ggagtatggt ttttattggt gccatttttg
    18481 tctgtcttga gatatcttaa aacctaaaag atgaagtagc gtataaaaag agtagataaa
    18541 ggtggtgatg aggcaagcaa aagggtgaaa aaaccaagca atatcgcgaa gcttaaaata
    18601 accctggatc gatttgaata gagccggaaa aaaaagaaga gaataaatag cgaagagtat
    18661 atttttatta tcgagtggcg gttgccattg gtaggtgcga atttttttgt aaaaataaat
    18721 atctttgata cgtcgtgatt gttttttgat gaacttggaa atagacgatt cacagtaggt
    18781 gtggataata ccaattttga ctttggcaaa gtaaattggc tttgaggttg cgttgagggc
    18841 agtagtgata atgtcaatat caaaaagata atcgccatta aaattgtttt ttaagaattc
    18901 tgatctgaag acggttccgt tggctccgat agtggggata gtttttttgg ggtggagggt
    18961 aattagtaaa tagttgtcaa attcggtggt ggggatgtca agaccggtcc atttgccact
    19021 tagggtactt tgacgatcgt agttgccaag aaaataagcg taaggatcat tggcgcctaa
    19081 aagagccgag taacgttcaa taaaaccagc atcgcgtcga taggtaaatt caattggttc
    19141 ggccccaata atttctgggg aagttgaaaa tggagatagc attagagtga gccaattatt
    19201 ttgagggagg atgttgtcag aatcgatgaa aataatgtat tggccggtgg cagatttaac
    19261 tccgacagcc ttaccagctt cggcggtttt taaggggttt tgaaaaattt tgcaggaaaa
    19321 ctttttagct atagataaag tttcatcgat agatccgcca tcgagaataa gtatttcaac
    19381 tctgtcttgg ggatagtttt gagagcgaat tgattgtaga caatttttta aaacattgcc
    19441 tgaattgagg actgggataa caatggaaat cttagaatat gccggcatag cgatgaacta
    19501 gagaaaaaat ccagtaatca acggggaaaa gaaagaattt tagtttccag cggatgctaa
    19561 ctaaaagcag agcaacataa taactaaaag taaaaatggc tcgagtgaag atgttgttta
    19621 ggcgggggta aagcatggtg taagtatcac gatccataat tccaaaaata tattgagtga
    19681 gcatggcagt aagttgggcg tcggcgtggc tgatccattt ttgacgaatg gtttcttgga
    19741 aggcattttc tggagacatg aggtaagtac tccaaccaac gagactcttg ggtgttttga
    19801 ggccaagttt gacagctcgg tcgtaaagtg gagctccggg gtaaggtagg aaaacataat
    19861 aggtataacg attgcggtta caaattttta gaagcttatc aatcagtccg agagtggctt
    19921 ggtattcgga agcaacaaac ttgttgttct ctgtcgaatc ttttgaccaa ggtagtccga
    19981 ctaaaaatga gtaaagaatt tttattttat acttgtgaca gagacgagta aacttaataa
    20041 tttctttaac attcatatct ttactaatca tgtctaaagc tgcttgagag ccggattcgg
    20101 caccggtgag aatcatggtg cagcctgatt ttttcattaa tttccaggta ctttcttggt
    20161 agcgacttaa ttggccaaca cgaccgttgg cgttacccca tttgatggga attttatatt
    20221 tgagtattcc ctgacaaatt tctctaactc ttttttcgtc cacaaaaaaa ttactatcgt
    20281 aaacggcaat tgaatcgaca tggtattttt ttactaagtg tcgccattct gatagaacaa
    20341 ttttagatgg aacaggactc caggttcttt tgttaactat ttgttcgaca caaaaaccac
    20401 aacgatgagg acagccataa ctagagatgt agtgggctgt cttgagaccg tattcggtgg
    20461 tctggtgaca tttttccata tcgacgagat gataaggtaa tggagggaga cgagacagat
    20521 ctgtctgacc tcggtcgggg gtagatatta ttttgttttt tgatttgtag actaagccag
    20581 gaatatcttt gaggtctgat ttattttcta gggcatgaac taattcggta aaagtaacgt
    20641 caccttgacc tttgacgatg atatctacat aggggttttt gattgtttca agaggcagaa
    20701 tagaagggtg ccagccaccc cagactatgg ggatatgagg atatttcttc ttgaaagctt
    20761 tggcaatacg aagaccatcc ttgatctgaa aaccagtcat agcactgata ccaagacaga
    20821 ccgagccttc ccctgttttg aggatttgtt taagatagtc tggttgaaga taattagcaa
    20881 agatcttaat atcgtaacct tgttgatcta aagttctgga gatagctaga agggctagag
    20941 gtgtcccttt ccactttctt tctagaggag tgggtgaggg aaaaaaaaga attatttttt
    21001 tgttagccgc cgatatatcc tttttcattt gtcttgtgtg gtgaaaaatc taagtttttt
    21061 gtgaaaaaac caataataaa gagtacaccg tagtagacgt gggtgcaagg tatggtaatg
    21121 gtagctaaaa gagagatgat agggcgatta ttttttaaga aactgagaaa ggtgataagt
    21181 agaaggaaga gatataaata gagagggaga ggaaatagaa tagaaaatgg tagatagata
    21241 acaaatagac tggggacgat ataacctatt cttaggcttg tttttggata tatacgagcg
    21301 aagttgccgc gatgaagagc gtagcgagta atttgttgga gatggggaat aacgacgggc
    21361 cgacgatggt ggtagacaat aaggtcagga tggtaaagta tttttttgtt gagcttgtag
    21421 acaaggttgg cacaaagaat agtatcttcc cctggccaat gatgagtgtc gaaaccttta
    21481 atttttataa agtcagattt tttaactata agattgactg aaggaaaatc atcgacaaaa
    21541 cgttgactgc taatagaatt acggtaagta ccggctcccc cactgccaag aaatgaagac
    21601 cagactaggc cggaagcttg ttggcgaatg ttgtctgatg gcggagtgag acagggtccg
    21661 cagacagcag atatattggg agtaaataat tttgaggcct gttgaagcca gttgggtgaa
    21721 ggataggaat cgtcgtcaag aaaggcaaga tatgtgcctc tagctttttt ggctccgaga
    21781 tttcttttga tagctgggct gacattaccg gatattttgt cggtaataat tatgatttca
    21841 aaatttgtat atgattgttg tttgaggtga gatatggttt ctttgagata gttggtgggg
    21901 attctgacag gaataataat ggagaaaaga ggtgttttgt tgttcatatt atccagtgtt
    21961 aattctcata tcaagctctt tatcgtaaac ccacttacgt ttatttttgt catcataata
    22021 gtggattatg cgaagcctgt aaaatacagc cagggcatcg atgagagaac gataaattat
    22081 tttaagtcca gaagcgtgag ttaggctatc gaaaacataa ttaagtttaa ttggtgcttc
    22141 gaatattcga gtaaaaccaa ggtggttggc gactgagagt atttcaaggt cgaaggcgta
    22201 gcttttgact agtagtcgag gaagaacttt aattaggact tttcttttaa atatcttaag
    22261 accggcctga gtatctctaa cttttaggcc gagaagaact cgagaaatta tctgagctcc
    22321 gaggctaata atttttcttt caaaaggata tttgacaatg gaggcggggt gccttttgga
    22381 ggcaacgata atatcggcct cataccactc catatgttcg agaatcatcg agacaccatt
    22441 ggggtcgatc tccatgccgg catcaataaa agcgacataa ttgcctttag tgtgggccat
    22501 accaaaacgg acagcgtagc ctttaccacg gttgtggcga tagccaataa ctttgagatg
    22561 gttgtttttt atgcttttgg ctatgcggta ggatttatca atagagatac catcaaccac
    22621 gacaattaat tcaaagtcat agcgaagctg actgaggcta gtaattatct gctcgaggtc
    22681 ttttttgatg gttttttcct gacggtatac agggacaata accgataaaa aattaggacg
    22741 ttttttcatc tagtagagtg agtttgtaaa gatagagagt ctggatggca gtctgttgcc
    22801 aggagaaagt tttggaacga gctagacctt tggtacgata ttcttgttgc agactggagt
    22861 cgctccaaac ttcttgaaga gctctattta aaccgttttt gctgtagggg tcgaagaaat
    22921 gtccattgaa atccataact tcgggtagag atgtttcatg actataaata actggagtgc
    22981 cacaactcat ggcttcaaca acaggtagac caaaaccttc agcaaaagat ggttgacagt
    23041 agatagtggc aagattatag atatggggaa gatcttcgtc tttgacaaaa ccgagacaac
    23101 taattaatgg agatttaatt gattggagcc aatggatgtc ctgagtccag ggatgaatgg
    23161 ggacttcttt tacagcagat gagccaacga taactaaggg atattttaat tgaagacaag
    23221 ctttaacgag agaagggaca tttttgttcc aattaatatc accgacataa aggacaaatt
    23281 tctgaggtag gttgtatttg ttgataggtt tttttatggg tcgaaaaatt gaatcagctg
    23341 cttcgtaagt gacaaatatt ctgtcgacag gatagttgag gtattcgcta ataagatgct
    23401 tggaatagtg agacacggtg ataatatggt tagcttgttg ggcaaggaaa cgctgaatta
    23461 accattttat ttcacctttg atgccaacag gataatgttg tttgaattga cgttcgataa
    23521 tgtcgtggat ggtgatgata gtggggatct tttttagagg gagggttagt ttaaaggggt
    23581 cgaagtaggg gtaatggacg agatcgtaat tttcaatttt taatttagaa tttacaatta
    23641 agtctatttg gaagttttga taatcaggat tgactttaac ttcatgttgg agagcatcga
    23701 ctaaatgttt ggtgtagaaa ccaactccac gcagagcatt gccatcatta agaggagaag
    23761 tatcgatagc aattttaatt cttttgtttt tcataaagct taatataggc aatgggaaaa
    23821 cgtaaggcag aataccaagc aaatacaaat ccaggaaaac catcaacata accgcgatgg
    23881 cggaaatagg ctaagacaaa ccaagaaatt ggtttgacaa cgaaatagcg aataaaattt
    23941 atcttggtga cagtgacacc ctcttgaagc aattgtgagg ctagtagaga agtgtaacgg
    24001 tcacttctta atagatatcg ggagaaattt ggatcggaga aatgaagtaa gtcttgggta
    24061 agatggccaa tgttaccgtc gatactagct tgttcgtgga catcgagaca gggtagcttg
    24121 cctttgccgt tgcgatacag ccggatagtg gcgtcggggt aaatacctcc tttagtaagg
    24181 aatttgccta agaaaaaatt tttgcggttg atccagaaac cgttttctgt cggattactt
    24241 tcgatagtac tgatgatttc tctttgtagt tcctgggtaa caacttcgtc ggcatcaagc
    24301 tggagtatcc agtcggaaga gcaggcttct atagccattt gtttgttgat atgaaagatg
    24361 ggtttgttgg tagtggagat gactttgacc tttttgaact ttttggcgag agagacagtg
    24421 tcgtcagacg actgaccatc aacaataatt atttcgctgg caaaagaatc gacggcttca
    24481 agacaacggg ttatattttc ttgttcgtta taagtagcaa tggcaatgga tattttcata
    24541 ttatttttca tatattttaa agctttgtcg ggttaattgg aaatcagacg aatcaataac
    24601 taaagaattt acaggaattt tttcggggat gtaaaaagtt atgttgtcga ttttctcgac
    24661 tgttgagaaa ccaaacctat cgggcttggt tagctgaatt tgattttgga ttttagtagg
    24721 gtcgtattta ctaaagaaaa gataaaggat gtagggttga tcgtatttat tggtaagata
    24781 aatgttttga tatttttgtt tttgtgattc gatatgagga acaataaggt caaatcccct
    24841 attccaggca aaaggatatc tttgaggagc atgaatgaaa taggaattga gaaaataagc
    24901 agttgaatac aaataaccaa taaccaaaaa cactgaaata agatttatga agtatgtttt
    24961 atgaataaga gttgataaat aatagatacc ggaggcggta agaatggata agggaatgac
    25021 cataggtaaa gcacgaaggg cggagggtgc ttggaatgtg agcgatgagg caatgggagc
    25081 aataaataaa aagtaaaaag tcaaaagtct aaaagtcaaa agtttagatt taattaaaaa
    25141 tacaagacca aggatcaaaa gagggagttc gatgagatag agttggccca tttcgggaga
    25201 gcgggaacgg ggaacttcgt cgccattgat aaaaagaaag ttgaggtcaa agtgagaagt
    25261 atatttttgg gcccatgaga gagtatagag aacgcgtcga ttgtgagtga tacgattaat
    25321 aagtttgacg ttgccgtgtt ggtttagaag ctcttcagaa cgggacatag ggccatagtc
    25381 ggcagtcaga ccgactccac caaagcgagc ggctccaccg ttatttaaga atgagacaag
    25441 gacagggatg gttaataaaa aggcaattat gattggaagt aataattttt tgtaattggt
    25501 aatcagtaac ttgtaattgg taataaataa aaataaaact agtaaagggg caatgaggcg
    25561 ggcagagtga taaatgtaga gagaagcgat aaaaggtaat aaaaagtagt tgaatttttt
    25621 ttgataaaac cagtaaatac caagaacgat aaaaaatagg gcggcggaag attcccaggc
    25681 tccacgagag aagtgaatgt gccaagggga aatgactaga acactagagc tatagagcgc
    25741 tagaacgcta gacttagaaa gaagaaggac taatttatac aggatgaaga ctgtcagaac
    25801 tgacaaaatt aagttgggga ggcggacggc tagaggattt agtccaagaa ctttgacaaa
    25861 gggcatggct aggtagacat agccgcctgg tttgaaatcg ccaaaagatt tgaagtgaat
    25921 aggccaaggg ttaccatgct catctttgcc agtttctagt agtgagtagg cgttgtaacc
    25981 taaagcggct tcgtcagggt tgagagaggg tgtgtggctg ataccataac cacgaagcag
    26041 gaaagctatg aggagtatag gaataattag tagttttagt ttaagcatct ttttttggtc
    26101 ttaagagata gacgaataaa gtaaaagaca gtaatgatat tatattggaa attgttcgaa
    26161 gaggggtgtt ggtaaagttt agactgaaag aatgggtgcc ttgagtaaat tcgatattta
    26221 ttagtccaag atttttatca acaaaaaatg ggtatgtttg gccgtctact attagagtga
    26281 aattgggaaa atagactatg ggcatagtta cagtagcctt aggtgaatat atgttaattt
    26341 gtccagatat accgagactg tttttgtaaa aagaggtaat ttcgccatta cctagggtaa
    26401 atactgggtc ggaggcatat tcattgggga agaggttacc agagttgggc caataatcac
    26461 gtaatccagc tccactttgg gcgattatat tttgaggaga gagtttttct gagtcagtga
    26521 gtgatgaata ccagatatct tctttgaagt aattgagatt taaaataagg gctagaggaa
    26581 ttattagata aattatcttg ggagagataa gtttggttat acttcctgag acaaaagcaa
    26641 aacaaattgt agctagacca agaaaacgcc aaggaaattg ataataagaa aggaaggaaa
    26701 tattttccca aataaaagta gatttgttgt gagtgagaaa tagagaaaat aatccgagta
    26761 aaaaagtaaa aacaattaat ttatgagtag ctaactttct tttaactaac aatagaacca
    26821 tagttaagaa tggaataatc cattgaaggt aaccaatttg aaaagacatg ccatcgtctt
    26881 caccccaaac tgagccacca aaaccccaga aacgagagag gaaaagttgt ttaagagtag
    26941 tgtaatgaag atggaagtca aaataatctt gggtcatagt cttgacattg gtgtagcttt
    27001 tttcaaaaaa ggcaggcagg agaaaaaagg aggacataag atagccaagt attaggtagg
    27061 gcaatcctga ctttagtttt ttttgttgga ttagaaaaag aagaattaat ggggaagtgt
    27121 agagtaaggt gaggttgtga ctaagagaga ggagaagaat aaagaatgaa agaagtcggt
    27181 aacgactgct tttgatcgtc cagaaaataa aaggaacaag tgataaagct aggttttcag
    27241 cgatggagcc acggacgtaa ctgttgagtg cttgataggg ggctagagaa aaaataacag
    27301 cggaaatcag gcctccagca ctaccaaaaa ataatcttga gaagagaaac ataccgatag
    27361 gacggataaa ggaagttagt ataaaaacca gcttaacact gtcaagataa gagaatccga
    27421 cagaatgaaa tagatgacct agactataag gtaaaggaga atagaagttg aagagtggat
    27481 aaccataacc aaatccccct tcggagattc gacgacaggg aacttggtga tcttcgatac
    27541 attgagtgta ctgctggagg cgaaaaacgt gcatatcatc ttgcattgaa ggatagccgg
    27601 tgcgaaaaaa tcgataggaa ccgattaatc cgagcaggaa gataatgatg atgactaatt
    27661 tatttttcac gaggctttag aataataaac tcttcttgtt cgatatatct tttatcgatg
    27721 gttttgatag tatcaaaacg atctgctggg acagcggcaa actctgactt ggggatgacg
    27781 ataatagatt tttgaggaaa agacaaagtc tgggaccatg ggataaatcc aaaaaagttt
    27841 tggttgtcta aagattttcc ggaaaaatat ggtgattgga agtcggcaag attttgagca
    27901 ggtgagatat tttgagctgg taaatagtga cggtagaaga aaaagaaagg taaaaatgac
    27961 tcatctttac tggaataaaa aacttgatag tcggaataat tactagtgaa ttcaagagtt
    28021 tctttaatgc cagtgtgcca ggagcgggct gataattggg ggtagtggat attgtagtaa
    28081 tgccagaagt tactaaaaga gagaccatag attatgatta tgaccgggat agttaagaga
    28141 tgttttttga aaatgtgttt aattccaatg gagacgaaaa agacaattga ggggagcatg
    28201 agaattaatc gggtggcgtg aggggaagag ctgtctctgg tgagtgaaaa tgggatcgga
    28261 gcgagaatta ataaccagaa gaagaaaata gataatttat taataggttt ttttgcaaaa
    28321 gaatagaaaa tacctattag taataagacg atatctatgg cataaatgtg acctagtttg
    28381 ccaaagccat ggcgaagatt accatcaccg ttgaggataa gaaactgagt ggaaaaagaa
    28441 gaaatatagt tattgataaa tttatcaagg acaatttgat atttgttgtg gaagaaaaat
    28501 gaagaggctg gtggagctac tccaatttcg ttttggtggg aggtatatat gtcttcgtac
    28561 ctcatttggt caacataatc agctttgtgg ggctcagaaa atatactgat ataggagaaa
    28621 cgaaaaccac ttttttgatt gatagtgtca actagcattg gagtcataat taaaagagag
    28681 aagatggtga ggagggaaat ttttctaaga ccaagtttaa caatatcttg acgccaaata
    28741 atcagaataa ggaggccaat tatagcaatg aagacttttg aagtgctgta gaaatatgtg
    28801 gagataagaa agaagaaaga agataaaaat agtttttgtc tttgccagaa atatatacct
    28861 agaagtatag tggtgatcat gccggagact tcaaatccag tacgactaaa atgaattgac
    28921 caaggggaga gggcaaaaac taaagcggcg atttttgacc tagtgattag ataaataatt
    28981 agggtggaga taacaccaaa aatagctgag ggtaggcgga cagaagtttc taagtcaaaa
    29041 aaatgttgaa ataaggcgat agtgtaaatg tggagtgaag tacgccagtc tgaaaaggag
    29101 tgaaaatggg tgggaaacag attaccaaaa taatcagtgt ggcgttggtt gaaaacatag
    29161 gcctggtatc cggcatcaac ttcgtcggag aaaaggctgg gggggaggct gttgagatta
    29221 gatgttctaa gaaagaaaga gagtaagaga atggcgagta aaggaattaa agtgatgaaa
    29281 ttaattttgg aaagcataga aggtagagtg ttggccgatg gggctaatgg gagtaagtag
    29341 ttttagatct atcccctgtc gaagtgtttc gtcccaggaa aaaattacca gaacattttt
    29401 tttatttttg agatcgttga attggggtga agtgaa
//
LOCUS       DDWP01000094            5496 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_27943, whole genome shotgun sequence.
ACCESSION   DDWP01000094 DDWP01000000
VERSION     DDWP01000094.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_27943"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1547 a         1464 c          704 g         1781 t
ORIGIN      
        1 ctcagtcaag tcaaagctct ctctccccaa gtcatccgta tcgaccatat ttttgattat
       61 tacaaagttg actcaggtaa tggtcaatac gacttttccc gactcgacgg agccgtcgat
      121 agtattttaa aaaccggtgc catccccatg ctttcactct cctacaccac caacgaaatg
      181 gccaaaaatg gccagaacgc cggtgaacca aaagactgga atcagtggta tcagcttgtt
      241 cgggctaccg cccgccacta ttctatagac aagaaaatca gtggtattta ttatgaagtc
      301 tggaacgaac ctgatctctt tggtggctgg cactatggta aagacccgtc ttacagcact
      361 ctctacattc aaagtgcccg agccgtagct gatggcgcca aaggcacatc ttttaaagtt
      421 ggtggaccag ccactaccgc ctactatagc aactggatca aatctctctt tgccactgct
      481 tcaaggaaca gactacctct agattttatc tcctggcaca agtacagcaa aaacctcgac
      541 gactacgaca aagattttga agctctcaac aagcttctct ccgactatcc ccaatattac
      601 gacatagaga gaacaatcac ggaagttggc cccaacccgg agccagattc ttggtacgac
      661 aatcatctct ccggtgtcca cctcatcgcc ctctctaccc gtatggccgg caagattcac
      721 cgcctattca ccttcgaacc agtcgatggc ccatctcctc gttccgacaa aagtagcggc
      781 tgggggctga ttactcatcc caataatggc gccaagccca agcctcgtta ctatgccatc
      841 caatttctaa atcgattcct tggtcaacgt ctcagcagca ccggcgacgg ttcctgggtt
      901 acttctatgt cttctcttaa tggcaaaact gttcaaatac tactcgccaa ctacgatccc
      961 cgaggtactc actccgagac agttcctcta ttaattacca atctcactcc cggccaatac
     1021 aaaatgacta tcactagcta tatgggcaaa tctactagta aaactattac tgccacagtc
     1081 aatcaccgtg accaaatcta tatggaacca aacagtgcca aactagttga attatctccc
     1141 cagtaacctt actacatccc caacagtaac taataagatt aagctcaaga gaactaccat
     1201 gccaatatta tttactatag cttcaaattt ttgattagct cttttcctag tcaccagttc
     1261 ataaagcaca aaaataatcc ttcccccgtc taatgccgga aaaggcaata tgttcactat
     1321 cgccaaattg accgatacta ttccaaagaa atgtatcacc gccaatattc cctgattttt
     1381 gttgatacta ctagtcgctt caaacattcc aatcggtcca gatacatctt taggagattg
     1441 acccactagc aaacttccaa tcatctttgt tactccttca aagattattt taccccaata
     1501 ataggcttct ttgaatccag ctgccacacc tttataaccc tcataccatt tcatttttac
     1561 catctcccga ttcgatatcg ccactcccat cgaaccttca ccttctggcg gattttctct
     1621 aacttgtacg ggcacccctt gtgggcgccc ttctatttcc aaaatcacaa tttttccttt
     1681 gtacttaacc acttcagcag ttagttctga cggactatta attaccgtcc cattcacgga
     1741 tttaatccaa tctccatcca tcaatcccgc ctccttggcc ggtgaatttt cagccactcc
     1801 aaccactttc actttatcag tatcagtcgg tactcccaga attgcataaa ccattgagaa
     1861 cacaaatacc gccaagatta tattcatcaa taccccaccc aggactatca aacccttttg
     1921 ccagggattc ttcgatacaa aagaagtact acttttaaga aattttttgt cagtcaggtc
     1981 ttcaccttca aggcgacaaa atccgccgat aggcaaccaa ttcaaactcc aaatagttcc
     2041 acccattttc ttaccccaga tttttggtgg taagcccaac ccaaattctt ctaccttaac
     2101 tccagttatt tttgctgcaa aaaaatgtcc aaattcatga accaatacca atattgagag
     2161 agctaaaaca aaaattaata tatccataag gtttattcta acaggtcacc aagtcagtat
     2221 cactatttca atcaacctaa caataatagt attattgctt atcgtgataa aaaccgctac
     2281 cgtactactc gtctttctct taggtataaa ggcaattctt actttccaat ccgttagccc
     2341 agtattctct aaccatgatc aagccagaga tatcatagct gctaaaattt tttcagaaca
     2401 tcgacaacta cccactaatt caccctattg tggtggttgt aatcttatca gcacactcaa
     2461 aaactcacaa atctattatc tagttcttgg cttaatatat tcaatcaata aatatgatag
     2521 cttacttatc ctctctgctc tgtgggccag cttaactatc ccgttcactt tcctcattac
     2581 ctatcaacta atccgacatt ttcccactgc catccttagc tctctggccg tcagtctttc
     2641 tccgctactc ctacatagtt cggtcaatgt cccctatcaa ccaagctatc ttcctacatt
     2701 ttcctgtcta atcatttggc tactcctatt aaaccgccac cgtcgatcca tttttctctc
     2761 cattctcacg ggaattttgt tattacttgc cattcaattt catctatcag ctctattact
     2821 acttcccatc tacttcatat ttacttacca gacaatcaag ataattcccg acaacaaaag
     2881 gaccatactt atactcttcc aaatcacatt tgtactagtg tttataaaat tgaatatcaa
     2941 cctcaactca acgtcgatct caccctccaa cctaacaata tccaacatac ttcaacactt
     3001 ttttatgcat ttaagtcaag acctgaccac tctttttcct caccccatac atccagtcat
     3061 ttttttactt attttattaa tatcaattat tatcaaccca caatatcgct tactccttcc
     3121 gcttattctc acccttccca taactggaat atcctcttat tacaattatt acaccgccat
     3181 tttctggcca tttttaatta ttctatctgt cggtatgttt cgcccccttc tcaattatat
     3241 tttctaccaa actataattt tgctagtttt tataatccta tctcccaaaa tcaccaccct
     3301 tctcccacaa cccatcacct cagcttccac caacaatttt gagctaatta aaagcatctc
     3361 ccaagagata gatcctcacc tcagccagtc caaaccaaat catatatatc aatacgacaa
     3421 ttatctaaac acttttaccg gttactactt agtacgtaac ctatcattac aagattaccg
     3481 agaaacactc aacgatcccc gctttaacaa gctaatctac tgccaatccc aatgttcaaa
     3541 atttgaaatt gaatttagcc gataccaacc agcacttctg acaacaggtg actaccgtat
     3601 ataccgtttc aataaataat ccttaccccc ttatttccac ttctttcttt ttagaaattt
     3661 cttctagccc accaatatat ttatcggtta ctttctgcaa ttctgcttca tctctcttaa
     3721 attcatcttc cgacaccgtt tttgcatttt ttgcatccat taattttttt ctaaaatcag
     3781 ctctagcatc tcttatcatg cctctagctc cttccaattt tctccccaac aattttacat
     3841 aatcttctct ctgctcctga gtcatcatcg gcaatgacat tcgaattaat tgtccgtcaa
     3901 ctaccggtgt catcccaata ttggctgcct gaattccgtt acatatttct cggataattg
     3961 attgatccca tggttgaaaa gtcaaacttc tcacatcagg cacgctaata ccccccagtt
     4021 ctattaattt attttttgtc ccaccataca cagtcacgac cacattttcg atcaaccctg
     4081 gtgttgctct acctgtccgg atagaaccaa tatcgttgac aaacaaatca gtgattttac
     4141 ccattcgggc attgacgtca gaaaaattta tcatatagac aaattatatc tcaaatctcg
     4201 ccatcctacc tacttttata ttttcaccaa atttaccaac taaagcagtt actaagtctt
     4261 taactttctt agaaggatct ttgacaaaat ccatctccaa aagttcttcg actgtttctg
     4321 ggctcatagc cgtaatttgc aaacacagat ctcgagtaac ttgaataaat tcctcattct
     4381 tagctacaaa gtcagtctca caaagcaatt ccaccattac tccaatttta ccggtagtat
     4441 gggtataagt agctactcta ccttggtgaa cttctttttc agatcttttt tccatctgtt
     4501 taatacctaa ttctttaagt aactccatgg ctttcttctc atcaccttct gccttttcca
     4561 ccgcaatttt tatctcacca attgagtacc ctgtctcccc tcttattttt ttaactagat
     4621 caattatttg tttattgtcc ataattcgta attaattctt aactcctgct gctttaatag
     4681 ctcgtccaat ctcttcaact atcaagttga tggatttaac actatcgtcg tttgccggaa
     4741 tggcaaaatc aacttttctt ggatcagcat cagtgtcaga gatagccaca gttttgatat
     4801 tgcgcatttt tgtttcttta acagctgttt tttcaaaacc agcatcgacg ataaaaagaa
     4861 catcaaacat cttttccaag ttagccaatc caccgatcat tttttctagt cggatctgct
     4921 cttttgatag atcgagtaat tcttttttag acttgtcagt aaatttgttt ttctccaaac
     4981 catctttaat atcattgtat ttacggatgt tcttacgaat ttgttcccag ttactgattg
     5041 ttccacccaa ccatcggtca gtaatataag ctacaccagc atcagtcgct actcttttaa
     5101 ttacttctct agcttgtcgc ttagtaccaa ggagagctat ttgtttaccg ttacgcctag
     5161 cattgtaaat aaagttacat gctttctcca agtcatttaa agtcttggtt aagtcaacga
     5221 tagatatccc gtctttagca atatagatat tttctttagc ttttggatga gtcttagaaa
     5281 ttttatgacc caagtgacaa ccagccgcca acaagtcttt caaaccaata gtcaattcat
     5341 actttttgtc ctctttgact ttaccttctg cggttacttc ttcttttaag ttttgagttt
     5401 taagttttaa gttttctttt tccaaggcta ccttctcatt cattctctta atattttctg
     5461 ccgtaacttt actctgagtc cttgccttaa ctacca
//
LOCUS       DDWP01000095            4882 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_14621, whole genome shotgun sequence.
ACCESSION   DDWP01000095 DDWP01000000
VERSION     DDWP01000095.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_14621"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1512 a          802 c         1032 g         1536 t
ORIGIN      
        1 tgtggtttct aactcgagaa tagctgagct tgatctatct tctggtaaat caatttgctc
       61 aaacagattg cttaaattat gccagccgga gacaacataa ttaactgatt caagctggtt
      121 tcttagggtt agagcaagtt caaaattgtc ttctttcgag gcccagtcca tctctttttt
      181 tagttgtctt tgaagcgttt taaagtgacc agaaagaatt ttttttattt tgccgatatt
      241 ttgtcgataa acttctggat cgggctgagg gccaggacag atgccaagat ggcaatagag
      301 acatctggta acaggatgtt tttttttgcc gtaataaggg aaaatatgac gaatagtccg
      361 aagaagtgat ttgacggctg tgccattggg gaatgggcca tagaggtcta cattatctat
      421 ggcatttgat ctaaagacag tatatatttt agggagagca tcacgagtga tacaaatata
      481 ctgataacta cgatcatctt taagcattga gttgtatttg ggatgatgtt ttttgataag
      541 agatgattcg agaatcaaag cttcaatttc ggaaccaaca acttttgtct caatagaatt
      601 aatctgggaa actaaggctt gggttttatg accaagagca tctttacctt gaaaatactg
      661 ggtgactctt ttttttagat taatggcttt gccaacgtaa ataatatctg ccgaagaatt
      721 tcggtaaata taaacccctg gttttgaagg aatatcctta atattgatca caaggtatta
      781 tataatgaat ttatgaagct taaggaaaaa atattaactg gtttgctagt aatactttta
      841 ataatctcag gaacaattcg atatttacaa acaaataact ttaatactgc cctaacttac
      901 gatcaagcaa gagacttact agatattcga gtactggcgg gatttcatga ctttcaagtg
      961 tctgggccaa caacttctat tactggattg aacttgggtc cattttatta ttactttaac
     1021 ttattgccgt tctgggtcag tggtggtaat ccacaggcgc tggtttattg gaatatattg
     1081 tggtttttag tagctggagt aatgatttac tggttcttct ggaagagaaa tatcgtccta
     1141 gggttcttta tttcgagtat ttttctaatg tcaccacaat tgttttcggt aacaagatat
     1201 ttttggaatg ccaatgcagt agtttacttt atcgtttttt attttctagg attatggaac
     1261 tttattgaaa ataatactaa aagaaatgca atcatcttag gaatagtagc tggactggta
     1321 attcaatttg aagctgcttt tggaagcttg tgtgtggctt tctcaattct ggtagtactt
     1381 tttagtagaa agaattttaa gaattattta tttggcttgt tgccgtggtt tttgccacaa
     1441 ttggcttttg aaataaaaca taactttaga atgacgaaat tgtttattgg aatgctgact
     1501 ggggaaaata caatattggg agagaaaata ccacttgatc aagtgttgag tatgcattgg
     1561 aaaactatta ttccgttttt tgaaggacag tttatgttgc cttatggggt agggtttgga
     1621 ctcttattaa ttgggctact ggcaatattg gccaataaaa attatcgaaa agttggttta
     1681 tatttgattg gctttatcac tttcgccttt gtttattaca cagcgatata tcatcatgaa
     1741 ttaaaaatgt ggtatctaga gggaattaga gtgtggtata gctttgtaat tggactagca
     1801 atgatagtgg tggcaaaata taaaaaaata ttttatgtac tggtcatagt gtttttagct
     1861 agaagctttt ttttgacagt cggagaccag cagaaatata ttagtgaaaa tggaaaaagt
     1921 aatgatccta aaaatatggc taacctagtt agaaacatag attgggtata tgaaaaaatg
     1981 aatggtaagg ggtttaaggc ttataactat gtaccagaag tctatgatta tccaaatcag
     2041 tatttgtatt ggtggtacgg ggtaaagaaa tacggttata tgccagaaga agtgtcttac
     2101 agtattactt acgtaccaga atatgtgcga atggagaaaa cattttatga aaaagctaag
     2161 ccagccgagg agggacggat tgctttgata tacgaaacaa agtcgactta taaagattgg
     2221 ctgggacaat ttaaagacta ttgtatagtc gataaacaag agactgcttg gaatatgata
     2281 gtcgaaacta gaaagaagtg cccagataaa aagtagttga ctattggatt cagatgtctt
     2341 ataatattta ttatggcaat tgaaatcaag cagggaaagc gtaaaagaac attagaagac
     2401 gggaatgtat ctgctgatca attaaatgat atgggtttcc ttgttcccgg tatgtgtctt
     2461 cgtggtctta gtactgggcc agatagagtg ataattgatg aggatatata taaagtcgga
     2521 ggaagtgcaa aaaaagttgt attaattcct gaacattata ttccaggcac gttgaatgaa
     2581 ggtttcgata atggagctgt gggtgcggtt ctgtgtagaa agggatcata tgtatcattt
     2641 ccgacagacg agggtgagat tagttttact ctgaagaaat aatgtgtttt ttgagatact
     2701 gaccggtata tgacttttta cagtcgataa tattttgaac agtaccttgg gcgacgactt
     2761 ctccaccttt ttcaccacct tcaggaccca tatcaactat ccaatctgaa ttcttaataa
     2821 cgtcgagatt gtgttcgatg acgacaactg tgttacccat atcaactagt cgacgaagaa
     2881 cttttatcag tttatcgatg tcataaaaat gtagaccagt ggtcggttca tcaagaatat
     2941 aaagcaaacg accatttatt tttttaacta attcgcgact aatttttaac cgttgtgact
     3001 cccctcccga gagagtattg ctgggctggc ctagttttaa ataacctaaa ccaacttctt
     3061 gaatagtggc gattttacga cggagtgaag aaatattttt gaagaaatcg gccgcttcat
     3121 caaaagttaa attgagtatt tcggcaatat ttttttcttt atattggact tcgagagttt
     3181 cgtctttaaa cctagtacca tgacattcgt cgcaagtaac gtaaacatcg gccaagaatt
     3241 gcatttcgac tttgatagtc ccctgacctt gacaggcttc acatcgacca cccttggtgt
     3301 tgaaagagaa acgaccagaa ttatatcctc gaatctgagc ttcagtacta cgggccataa
     3361 gggcacggat atcgtcaaag actttggtat aagtggctgg attactacgg ggagtacgcc
     3421 caatgggaga ttgatcgact aataaaacat cagataaatg ttcggctcca gtgatcttgt
     3481 cgtatttacc aatagttcca tagtaggcac ccaaggtagc tttacggaca ccaccataaa
     3541 gtgtgtcatg aactagagtc gatttgcccg atccagaaac accggtgaca ctaattaatt
     3601 tattgagagg aaatttgaca gagacgtctt tgagattcca ttgtttggca ccaactatct
     3661 caatccccct agcccccttt gacaaggggg aaatgtgtga ttcgatctta agctttccgg
     3721 acatgtattg cccagttaaa ctttttttac ttttcaaaat atcatctagt gagccttggg
     3781 caattatttc accaccattg gtaccggcgt gcagtccaaa atcaacgaca tgatcggcac
     3841 ttctaataac ctcttcatcg tgttcgacga caaccactgt attgccaact tcttgaagtt
     3901 tttttaaagt accgataaga cgatcgttat cgcgagaatg tagaccaata gttggttcgt
     3961 cgagaatata gaggacgcca gttaagccag taccaatctg agaagccaga cgaattcgtt
     4021 gagcttcacc tgaagagaga gtccccgctt cacggtcgag tgatagataa tctaaaccaa
     4081 cagcaagcaa aaattctaaa cgtgtatgaa gttcgcgtct gattggctca aaaatttcct
     4141 tatttttgtc gctgactaaa agagaatcga gggaatttat ccaagaggat aattgttcaa
     4201 tgggtagctg gcatatctga tagatatgtt tgccatttat gtggatagat aacgcatcag
     4261 ggttgagacg agaaccatgg caaacagaac attcttcctt aatcattaac ttttccaact
     4321 cttctcggac agtatctgaa gttgaagaaa agtagcgaga ttctagatat ttggctttcc
     4381 attcggggaa tttactccga tctatctgaa gtttgttacc cagaccttta cagtcgggac
     4441 aagcaccttg aggtgaatta aaagagaaaa gtcgaggctc aataatgggc aaagacaaat
     4501 tacaggtagg acaagcaaag ttttctgagt aaagggtatc taccaaatca accggagtgt
     4561 cgggaaaatc aaaggagaca tcattaattt tggcgacaac acaaagaccg ttgctaagtt
     4621 tcatagcttg ctcggaacta tcgacaagac gagagaagag gtctttctca ttttttaaag
     4681 tatctttgtt gattgatacc cgatcacaga cagcatcaat attgtgcttg ttggtcttaa
     4741 cgatgccaaa atctgaataa agatcgatca ttttgccatc gacacgaatc catttgtaac
     4801 cttgcttttt tagattttcc agaagaccat gaaagtcacc agctttattg cgaatgagtg
     4861 gggctaaaat aaagaaacgg ta
//
LOCUS       DDWP01000096           11940 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_22199, whole genome shotgun sequence.
ACCESSION   DDWP01000096 DDWP01000000
VERSION     DDWP01000096.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11940)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11940
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_22199"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3942 a         1364 c         3113 g         3521 t
ORIGIN      
        1 tttaattatt atcgtcaaca ccagaatgaa aagaaatatt ttgcttatct taaaaatgct
       61 gaagaattaa atagtgtgtg ggggcaggat ttgcctagct tcgaggaagc gattggtaga
      121 cttttagata acgaagaatt gatcgaaaaa gtatttattc atttgcggga tagtacgtat
      181 tttaaagagg tgtgggaaat taagccaata gaatatttgc atataaattt attattgttg
      241 atttatcgaa cgacaattct gcaagctaat aaagatatat taatagatca aaatggtcaa
      301 aatagagttc aaatttcttc gacaattgaa gagatggtgg cttgtttgca gtatgaggga
      361 aaagacaatc gattgtttcc ggcttttaaa cattggttac aggattggaa gatagaggat
      421 tttgatgaaa gattgtatct tttgttatag gatacttgat gttataataa ttaatggttg
      481 ataaggttgt ttttagtaaa actcagcgat taattgctga tgatagattg gaaagagtga
      541 ggcataaagc tgacgaggaa ttgtcctttt gtttgccggc atctatttat caggtagctg
      601 cccgaattaa tccttcaggg ttgcctagac cagagacttt tattagagag tgtagtcgat
      661 tagagatggg tgataagact ggatcagtgt ctcctgaatc tgtggtagag gcattggttg
      721 tagttgctag gccgttaggt gttagagtcg cgagaatctt gtctggccaa gcgtttgctg
      781 agcagttggt ggatagtggt gttaaaaagc caaatcgcgc acaaggtaat aaaggtagat
      841 catgtggaca cgcagagttt atggatgata ctcaaaggac taagattcgt gaatctgaac
      901 ttaatggcga gggttggcgt aacgtggcgg taattgagtt tgagatgtct gatggtgctg
      961 aggttcaaac cagacacgaa gtggctggta gaatcgagag aacttcaggt agttgtaagg
     1021 tatcatggcc tcacgttcgt gtcttggcta gaaaagtaga tctctcttag agggtaagat
     1081 tagttataat ctgaccatga aaacaaaata cattcttcat ggtggtaatg ctaacgatcc
     1141 atgtgaggaa aatgataaat tttttagtga gattttgaaa gatgcgggta aagaggtgtt
     1201 gattcttctt gtaccttttg ccacaactcc tgataaatat gagagagaat ataactggaa
     1261 tactagccag tttgaaagag ttagaggtga gagagtgatt aaatatactc aggctaataa
     1321 agaggatttt ctggaacaga tcaaaatggc tgatgtaatt tacttgagtg ggggaaagac
     1381 gacaaatgta gtagaagctt tatcgaatta taaagattta gaaaaattgt ggatgggtaa
     1441 agtagttgcc ggggagtcgg cagggatgaa tgttcttgct caatatggtt atagtctagc
     1501 ggcagataaa gtttttatgg gtttaggaat cttgccaatc aaaactattc cacactatag
     1561 agaagaattt gcttataagt tggatggggt gggagaaggg ctagagttac taactttagt
     1621 cgaacatgaa tataaagtga tggaggtata attggaacaa gatgaaaata gtaatgcttt
     1681 attcaaaatc gaggttaaga gttgaagatt taaaaaagct tgaagatgtg ggggcaacat
     1741 tttacgaaga aaaagggaat aaattagaag atataaaaga actttatcaa gatgaagaag
     1801 tggtcctggg aatagcaccg gggtggattg aggggcggtg ggaagggttt acttgggata
     1861 aggtaaataa atttaaaaat ttaaaggcag tttgtacctc gacaacttca tatggttgga
     1921 taccgtttaa agagttggcg aaaaaaaata tccccgtgtg caatgttcct ggaaaatcaa
     1981 cagatgcggt ggcagaatat tatgttttta tgatgacggc attgctacga aattatccga
     2041 aaattataaa aaataattgg ttatctaatg aagcagaagt tgggttgggg actgatgctt
     2101 gtggtctaac tgcaggaata attggtctgg ggagtattgg tcgaaaaatt gctgatttat
     2161 gtgcaggcta ccaaatgaat attaaatatt ggagtcggaa taaaaaagat tgtgtttatg
     2221 aatatttgga tttagatgaa ctttgtaaaa aggttgatgt tattttcttg actacggtgg
     2281 gagatgaatc aaccagagat ttgattaatc gacatagaat taatctaatg aagaagacag
     2341 taatattttt aactccaatt gatactattg cttacgataa acaatattta attgaaaaag
     2401 ttgggaaagg agaaattgga ggattgggtt ttgaatcaaa gactgaaaaa gtaactgatt
     2461 ttgtaggcaa tgtgtttccg gctccagaat tggcatatta cactagacag acattagaaa
     2521 aagaatcaaa aataatgacc gaatcaatta tgtcggttgt aaacagcaag ccagttaatg
     2581 tggtgaattt ataagttaca atgagacaag atgaaaatag ttatatctga taaattggac
     2641 atttctgatt ctggattaag tgatttgaag aagtattcag agatcgaaat atataatgat
     2701 gttgtaaatg atccggtaga gatcatgaaa agaattgagg gtgctgaaat aattacagcc
     2761 aactacattg acattacaag ggaggtaatt ataaattctc cgaacttaaa atatattatt
     2821 tcgccggcgg ttggatatga ttggatagat gtgaaaactg ctacggaaaa ggggattaag
     2881 gtggttaatt gtccaacatt caacacatat gctgtagcgg agcatgcaat tggattgatt
     2941 ttttcggttt atcgtagggt cgtggagggt catctgcaaa tattagaagg aaaatggcaa
     3001 tcgatgcagt taactggtca tgaagttaaa aataaaaagt ttttgtcgat tggttatggg
     3061 aatattggta aacctttgtg tggtttggcg agtgctttgg ggatggatgt gaaatattgt
     3121 gatagtaaaa ctgctgaagt tgatgttaac aaattgatat ctgaggcaga tattgttgcg
     3181 ctttgttacc cattaacaga gaagacgaaa ggttcgtttg atgctaaacg catttcattg
     3241 atgaagagta gcgcgatttt aattaacgtt gctagaggaa gagttctgga tcaagatgct
     3301 ttgtatatgg cacttaaaga aaataagatt cttggggcag gcttggatgt gtttaatgat
     3361 gatgccactt ttactaaagc aaatgaagag attataaatt ttgccaagtt accaaacgtg
     3421 gtagtaactc cacatgttgg ttttaacaca gaagagacaa aggaaaggtt tggaggtgaa
     3481 ttgataaaaa atattgatgc tattttggca ggtaatccta ttaatgtggt taattagtca
     3541 aatttgataa aataggggat gaagaaattg gtgctttatc ccaatagaat attgagagag
     3601 gtaactccgg agataacagc agtagatgag aagctactca aggatataaa tgatctaaga
     3661 gaagtgttgc taagtaaacg ggggcgagca gctggactag cagctccaca gataggttta
     3721 aacagaagat tttttgggga tatatcccaa ggcaaaaaag atcttaacct gtatattaat
     3781 ccaaagatat tggcaactta tggtgataaa actcgtccaa tcatgacttt tgatgatggg
     3841 acgaaagaac cttttatgga agggtgtttg tcgtttcctg atctttttgg aatagtgaag
     3901 agatacctga agattgaggt tagttgggat gaagtgggga aaaacttaaa acttaaaaca
     3961 caaacatcaa aactggaagg gattgaggca atagtttttc aacatgaact tgatcatttg
     4021 gatgggatac tgtttgttga tcatatctta gaagaaaaag gagacctgtt taagtttgat
     4081 ggcaagagac agatgaaatg ggcggtagag aaagtgattg aggattagga tctccgtttt
     4141 acatcttcgg caaatgctaa tgcttcagcg ataacttgcg ggtctgagac taccgctttg
     4201 aggacgtcac caagatcggg ggctatttca cccattttca taagtatgga gaaaagaccc
     4261 ttgtttgggt agccttctgc agtttttaga acggcgtttg aataagctag gcatagacct
     4321 gcaccattta attctggatg actatcctgc gataactcgt cgacaaaatt gcgaaggaat
     4381 tgagcttggg gggctttgct ttcatcaaat gtttctctaa gctttgaatg tagttctctt
     4441 ctgtttggta ttaaaactgc ttttaattct tttcccatat aatgtggatt ttattctatt
     4501 ataggatgtt tgtcaacagt tgatgtggtg attttggcta gataatcttt ggcttcgaga
     4561 ttggtggggg cgataagaat ggttttttca aacaaggttt tggctaattg gtattctttt
     4621 ttattaaagt gaattttgcc taaagcaaat agagcgtggt cgtaatttgg tttaagattg
     4681 atagcttttt gataattttt ggcggcagaa tctgtctggt taatcctgtc atagaattca
     4741 gctagtagat agaagctcat ggcgtcggta ggagcaatct tggtggcttt agtaagtgca
     4801 tcgatagcca aaagataata atcgttgtct aaagcagaca gataataata agcttgagct
     4861 ttttctttcc ataggtttag attgaaaggg gagatctgag tggctttttc aagagagatt
     4921 agagtggggt tgatatattt ttcatcttga gttgttaggg ctagcttggc agataaagtt
     4981 ccttggctaa tgagataaac tggctcgtct gggcgataat taagactgat ctggacactt
     5041 tcaagagcgt cttcatattt ttgttgacgg tcgtaacttt cagattgagc gtagctgata
     5101 tcagctaaat aatattttaa tatttgaaaa cctagaaatc caaatattaa aataagaaat
     5161 aagaaataag atttatgatg tatgaataat tttttgggtg tggattcgag agcaggaatg
     5221 gtaagagcgg ggaggaggaa gaaatagaga gaaacaataa cgatggaaaa accagcaaaa
     5281 ttggttatta aaattgataa aaaaggtata aggtatatgg tgcgtcggag atggtatagg
     5341 ataaataaaa ttagaaccat gtaagtaata aatccgaagg tgccggtggt ggctagataa
     5401 tttaaatatt cgttgtgggc tttgttgtag agaaaattcc attcggaggt aagattatga
     5461 gcggctggac gagtccaata ataggcgtca gcaaaagttt ctgggccagt accaaagagg
     5521 ggaaattctc gccagatatc gatggcgcct tgccagacaa tggtgcgaat atcttcactt
     5581 ttagtaatat ttaaactaac aatttccaaa ttaacaattt ctaaattttt ttgaggaaac
     5641 agtttgtctt taattgggtt attgattatt aaagacaatg caattagtaa tccgtaaata
     5701 acaattagta attttttatt ttgatttttg aaaaaataat aaatgccata gattcctaag
     5761 gagataatgg cggcgattat tcctgattta gattttgtat ataaaagaca aagataaaag
     5821 attgaagtgg gtaatatgaa gtaagattta tgaatagaac gatgaaggga aagggggagg
     5881 agaatacaaa gataggcagc tagccaattt ggttggccga aggtagagaa aacacgggcc
     5941 atgacatctt gctgccaaaa gtttttatcg ataccaaaat gttgaagaat gccataggtg
     6001 gcaactatta accctgataa tagcgaataa tttataattt tttcttttag tttgtcgtta
     6061 agataaacaa ttagaatcca atacaaaaga gcgtaagaga taagagataa gagaccgccg
     6121 ttgagtcggg aatagtaacc aaagatagaa gtgtggggat tgatactaaa tatagtagag
     6181 acaatttgag agacgatgaa caaaataagt ggtatgtcta gaaaactttt tctaaacaag
     6241 ggggctttac cattaaggta attgaatagg tgaacaatga tgataactag ggtcagacta
     6301 tagacaaaat acattttggg tagttcaaag atctctgagt ttgagagaga cattaccagt
     6361 ggtgtcagga agaaaagaag tgaataaagg atagacatac tagtcatagt atacttgaag
     6421 tgtgaagaat cttttgagct ttatagaaaa tttctattgg gatattggag tggatttggg
     6481 aactagcaat acgctgatat atttaaggga taggggggtg gtgatagaag agccaactat
     6541 ggtggcgagg ttgaaaagga aaaggtgggt ggggatgtct gctccaaaat ggaaaggtag
     6601 tgtgtcgatt gcttacgggg ataaggccaa ggaaatgtta aaccgtgagc cgaaacagct
     6661 tgaagtaata tcacctataa aaaatgggat gatatctgat ctagaagtat tagaaaatct
     6721 ggtggcttat tacttaaaat tgatttacga aattcctagt cgttatccaa agttatttaa
     6781 accaagggtg gtggtatcca ctcctggaag tataaatgat gtacaaaaaa gagccataag
     6841 atcggtattt atggctgctg gagctaaaga agtagtctta attgaaggag tgatattggc
     6901 ggcggtgggc ttgggtttac cgactgaccg atcgtcaggg ttgatggtag tcgatatcgg
     6961 tggaggtaag actgaggtgg gggtggtatc gatgggagga ttggtggtag gaagaggaat
     7021 caaatcggcc gggacaagta tggacatggc gattataaat tatgtgaaga tgaaatatgg
     7081 acttttagtc ggtcagaata gcgcagaaag aatcaagatt gagctgggaa acgtgtggga
     7141 gaaagaaggt gaaagtctta aaacagcaat tttaagaggt cgagatttgg aaaaaggttt
     7201 gcctagagcg gtgaagataa attcttctga gattcgtgaa gcgataattt tcgaagcgca
     7261 aaaaatagtt aaattggtta aggaagaatt ggacgagacc ccgccagaac taatggaaga
     7321 tgtactgaaa agaggagtgg tcctggtagg taatggagca aaattggctg ggcttagtaa
     7381 attaattgag aaagaaatta aaattagtac aagagtggca gatgaggcgg gcttggcgat
     7441 tattaaaggt tgtggagagg tcctggaaga cagaagcttg tttgatagga ttagaacagt
     7501 atcaggaatt aggagatgag aaaagattat ttacaaattt cttggaagca gggactgtta
     7561 aattggggat tggtgttgag tgtgggaatc ttgatggttg gagtgatacg attgtcatta
     7621 attaagggga aaatttatca gagtttggcc agagagaata aagtggaaga gacaagaatt
     7681 ccggccagta gaggaaagat actagacaga aaaggtaggg tattggtgga aagtgtctat
     7741 cgttatttta aatatgtaga gggggataaa gcgtatcagg gttcaggaga atttgatggt
     7801 tataagtttg agggtaaaga cttggcgtat gatctgaaaa gaaaatattt gtatacagaa
     7861 agtatgggat tgttgacagg ttatgttggt ctaccaaatg aatcagaact taaagggggg
     7921 aagtgtggca gtaaaattag tagtgcagag atggtgggac gaggagggat agaagatggg
     7981 atggattgta aattaagggg ggtagagggt aagagattgg tggaagtaga tgcaatggga
     8041 aaatatgtaa gagagctggg tagagcagag cctgaagcag gagaggatat tcacttgagt
     8101 atcgatgctt tctggcagga caaaatatat aaattgttgg atgggaggaa agcgtcggta
     8161 ataattagcg agccaaatac ggggaaaata attagcttgg tgagtagtcc tgggtttgat
     8221 gctaatgctt tttcttttaa tcaagataat gtgactatta aaaattactt agaagacaag
     8281 gataatttgc ccttacttaa tagatcgatc gcggctaaat atcatccagg gtcagttttt
     8341 aagatggttt cagcgacagc tggattggaa agcggagtga ttgataaaaa tactttgatt
     8401 gaagacactg gggtgattaa gataggtgaa tatagctata acaattggtt gtggacaaaa
     8461 agaggggcga ctgacggaat ggtggacgta gttaaagcat tgaaaaaatc gaatgatatt
     8521 tatttttatc ggttggggga gaaactggga gtggacagaa taaaagaatg ggcagagaaa
     8581 tttggttatg gagctaaaac aggagtggag ataccgggtg aggtggctgg ggtggtgccg
     8641 agtgatcaat ggaaaagaga ggtaaaggga gagagatggt ttctgggtga cacatatcat
     8701 ttggctatag gtcagggatt tcttgataca actccgatac aagttaattt ggcgactaac
     8761 gtgattgcta ataatggaac aaaatgtaaa atgagtttgt taaaaaatgg ggaagctagt
     8821 tgtgagaagc tgtcgataaa agctgaaaca ttgagaacaa taaaagaagg gatgttggca
     8881 gcgtgccaaa gtggggggac ggcctggcca ctatttaatt ttaaaactaa aattgcctgt
     8941 aaaacaggga cggctgaagt cggggatggg agtaaagata tacatgcctg gttgacggct
     9001 tttgcaccgg cagataaccc ggagatatcg attactgtat ttgtggaaag gggcggtgag
     9061 ggttcggacg tggcaggacc aatagttggg gatatattaa aagaatggtt tgaagaaccg
     9121 gagacattag taccgaggta taaaactaat gagtaacaaa aaaattgcaa ttgtagggtc
     9181 aagaaacatg agcagctatg gtaagcaagt catagagatt ctaatgaaag atttaaaaaa
     9241 tcaagaaatg gtaactatca atgttagtgg ttgtaaccga gagataatca gattgggagc
     9301 gaagaaagtt tttagtggag agaattttga aaagttaaat gaggaagtgg cggaatatgc
     9361 ggattgtcta gtaattgtgg aaggtgggga gaaaagtggg acactactat tagcagagaa
     9421 atttgtggag aaaggtaaaa cagtttattg tgtgcctgga agaatcactg atgaaggatc
     9481 gtttgcccca aactggttga ttgctcaagg agcgacacca ttgattgata taatactctg
     9541 atgaataaaa caaaaaggct tttgggatat tcgttagtgt tgacagtgtt gtacgtaatt
     9601 tggttattac caggatattg gaacttggga tttacaataa ttaattcttt gtttttggga
     9661 gttgttttaa aagaatttaa aaaagataat tttttgagaa tattatgggt gttgagcttg
     9721 gtattgggtg gatttatggc aataagaagt tacccttttg taagttttct aacgtggata
     9781 acaataataa ttttagatgt tattttggtg gctttgggaa aaaatccaaa agcgatgttg
     9841 agttttggta ggttgatcgg gactaattta agagtattaa aaggagcgct agaaagttgg
     9901 aatagcttgg tagcaataat tttgagagtt aaaaacagtt ctgatgaggg attgttgaaa
     9961 aaaatattgg ttggagtgtt gatgtcgatt cctttattgt ggatttttgg gacgttattt
    10021 tataatgctg atccaatttt tgctaagata attaatgaga ttaggtggcc aaaaattgag
    10081 attcatgcta gatggtggtg ggatttttgg gggacaataa tattttttgg agcagttggg
    10141 ggaattttaa ggaataaact agttaaacag gttaaaaata caaagtggtt aaaatcagta
    10201 acggagttga atgtagctgt attgatattg gaggggttgt ttatcgcttt ttcggtggta
    10261 cagataaaat attttttggc aacacctgat gatttgaaaa aattgcagat aattttttca
    10321 gaatatacta ggtcgggtta tggacagatg atatttgctt cagtattggc atatgtggtc
    10381 gttttgcggt tggagtttgc ccagcggtcg ataccaaaca agttgaccaa gtttttgatt
    10441 tgggtaatga tgggagagat tttgattttt ataattgcgg ctactaagag aaactatgtt
    10501 tatcaatcat tttatggatt tacagaaata aggttgttgg gatttgcttt gtcgatatgg
    10561 ttggtagcaa tgttgttctt gttggcttac aaagtgtggc aaaaaagaaa ggctaacttt
    10621 tttactagag gaataatttt ggtaacagct tttagtattt tgggattaaa tgtttttgat
    10681 attgatggga ctatagttcg gatgaaacca gcaagattgg attgggggtt ggattatgat
    10741 tatttgtcct atttatcgga cgatgcttgg caagggtggg agaaaatttt gttgagtact
    10801 gaagagaaaa taaaagatga tcagttgaag gattttgggg aaataatgat gcaaattaat
    10861 ttaattaata ggttgaaaga tagacagtat cgtactgttg ggatacaaaa taattactgg
    10921 aattggggtg gaagctttaa ttattcgaca atgaaatcat tagattatct aagaaagaat
    10981 gaagggagga tgagggaaat tttgagcaag ctggagtttg aaaaagacaa atttgagcaa
    11041 aaacagagag aggaactctt ttcaaaatgt ccagaaaata tttttaatag gttgagtgag
    11101 tattatccgg gaaaagtttt ccgaagtaat aacaatgtat tacttaaagt gggtgcacag
    11161 atgagctcga acgaagaggc taagatgttt actagagttg atgaagcatc aggaggaatt
    11221 aagtggagat ggtcttggag tggttcgata acaaataaga cttttgctgg ggtttatacg
    11281 ataagctgtg ttcgatgaga ctggacttat agatgaactg atggtaagat acgccctaat
    11341 atggtaaata agaatttgtt gccaaagaaa gtgattgggc tttttggttt aaataatcct
    11401 ttagatttgc caggggttaa aaatcccagg ccgccgatag agagatatcg gattgataaa
    11461 gttaaggggc cggataacca accggcgttg gcatcggctt ttgtaaatcc gggagatgtc
    11521 ttaggacgtc gaccggatag aaaagtgatt ttagtggata gtcctagggt caatccagac
    11581 agtcctggtt atcctggaat cggtttatgg ttggagttaa ctgatcaaac tcaagcggca
    11641 gtgctgatgg caaagggatc tgttgaactt gggatgagaa aagtcattac tccagaattg
    11701 gcgaaaagaa taagagagca aaatctgacg ctagcttcaa aagcaacagg gcttcatggc
    11761 gaacatcatg ttcgggttga attaacgaca ttgtctgatg gggcgacagt tgtaaagagt
    11821 gatttggtgc catgtagtgc agattgtcca tgcaaaagac gcagtttcaa tagtaagcgt
    11881 aggtaaagct tgatgtgtcg gcttgacaga gggtgttatc atagacgaca atggctaata
//
LOCUS       DDWP01000097           14939 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_13426, whole genome shotgun sequence.
ACCESSION   DDWP01000097 DDWP01000000
VERSION     DDWP01000097.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14939)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_13426"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         4553 a         2736 c         3362 g         4288 t
ORIGIN      
        1 ttcctgtcgc tctttaaaat tcagcgtgtt aaaaggagaa gaaaatggaa tggatttttc
       61 aggtaattag cgatctgttt gcagtttatg gagttgccgt cttgtttaaa gcaatcgaag
      121 tcggtattca cctatttgga ggataagaga tgagtgcatt cggaccacca cccagtaggc
      181 caccgttgag gccgtttcca gagccgcccc cacttcctag tgggtcgcca gttaaagccg
      241 agccaaatta cacaactaca ctattgattg ctaacttttt gttgacatca atgttgtgtt
      301 gtctgtttct ggccggattc acgtttctac tgattagata ggagaaaaaa tgaaaaaagt
      361 ttttgaaatc ttcaaagccc gtaaaaccca gattttaact ctggttatcg tgattttctt
      421 cattgttttt gttggtttga acactcaagt ctattacttc gtcgatgagt gcggatcata
      481 ctggccagaa cagcacaatc catgcatcgc ccacccgtgg tcgtcatacg aagtccaagt
      541 ggaaacagag attatgccac tgacttggac aagattaagt cttaataacg attatgaaga
      601 tggttttcga agtttgtctg ttggattagg aatagtggtc gattcttgga ctgattaaca
      661 cgctgacacc agcactctat tatgaggagg gcttttgccc tcctcctcta atggatgtga
      721 ctggtgataa atgctttcca gatatcatga cttcgtcacc atctggactg atgagctcgg
      781 aagagcgaaa gcataaaatg acagaactaa aagagataat tagttatttg ttggaaaggt
      841 ggcggagata ttggtcatga gttactttga tatattttat aaactaatga tatactcgct
      901 caaatgtcaa aggagactcc ttgtgaaata tttgcgagaa gactgattgt caaaaggcca
      961 gtgccaacaa agcctaatgg aattgaatat tattcaggag aaggctcaaa ttatccaaga
     1021 agttacctag acccagagtc ttctcataga tatagatttt gggatatggg agattggatt
     1081 gcgagaaaat taccaggtga ggccaatatt cttgatgtag gaggtgcact aggacatctt
     1141 agttattgga tgaaaagaag acaccccgga ataaaagtga ttaacagtga ttactcagta
     1201 gaagcattaa aaagaggaag agaaaatgga gtggactcac ctgttgggtt gtcagctgat
     1261 aggttaccat ttaagaaaga ggcatttgaa ggaatagttt ttggagatgt actagaacac
     1321 ttaactccca gacaggctga agcatcacta aaagaaacca tgagagtttt agtgcctgga
     1381 ggaatggtct ttattaatat accgaataaa gatacgtgga caaaaatagc atttgaggaa
     1441 cctactcacc tgtggatacc tggtataaaa gatatggtag acagcttgaa agtacttggc
     1501 tttgaagaaa taaattcgtc tactcgaggt tttccgttta gttttggctt aagaaaagtt
     1561 attgacagag atatacactt accttttttg ggcacgtcaa tttttgttag tgccagaaag
     1621 ccaaaagagt aaatctttca ctactgagta agtaaggcgg cttgggcagc ggcaagacga
     1681 gcgatgggga cacgataagg actacaggaa acgtaattta gaccaatttt atggcagaat
     1741 ttgattgttt ctgggtcgcc tccttgctca ccgcaaatac caacatgaag atctttgcgg
     1801 actccgcgac ctagcttgac acacatttcc atcaatttgc caacaccttc ttgatcaaga
     1861 gtcttaaacg gatcttcagg aagaattttt ttatcaagat agtcttttaa gaatttacca
     1921 gagtcgtcac gggaaaaacc ataggtcatc tgagttaggt cgttagtgcc aaaggaaaag
     1981 aactcagcct cagcggcaat ggcatcggca gtcagagcag cacgaggaat ctcgatcatg
     2041 gtaccgagct cataagtcat cttgacagca aattttttga taacaccgtc aataacagga
     2101 atgagacgat ctttgagcca cttatattct ttgatttcac cgattaaggg aatcataatt
     2161 tcgggaacga ttgattcacc agaagtgatc aattcacaag cggcagtggc gatagcctca
     2221 acctgcattt cataaatttc ggggaaagta acacctagac gacaaccacg atgacccatc
     2281 atgggattaa attcatgaag agattctatt ttctcggtga gcttgtccac agatatatcc
     2341 atctctttgg ctaaagcttc gatatcttct ttggactgag gtaaaaattc atggagaggt
     2401 ggatcaagta gacgaatttt gactggtagg ccatgcatga tttcaaaaag ttctctaaaa
     2461 tcgtttttct gcatgggaaa aatcttggca agagcagttt tgcgttgctc taaggtgtcg
     2521 gaaatgatca tctggcggac agcaaagata cggtcggctt gaaaaaacat atgttcggta
     2581 cggcaaaggc caattccttg agcgccaaaa tcacgagcaa cttgggcatc gcgaggagta
     2641 tcggcgttgg tgcggacacc aagagtacga tatttgtcgg accattccat gatagtacca
     2701 aaatcaccac taaggactgg agtaatagtg tcaatcttac ccaaaaatac ttctccagtg
     2761 gaaccatcga gagtaataat gtcaccttcg gtaataatag tgtcaccaac acgaagcttt
     2821 ttttcaattt cagaaacatt aatagattca cagccagcaa cacaacattt acccatacca
     2881 cgggcaacaa cagcggcatg tgaagtcatg ccaccacgag cagtcaaaat accttgggcg
     2941 gcatccatac cagaaatatc ttcggggcta gtttcagctc tgactaaaat tactttctta
     3001 ccttgttctt tccaatccac agcagtgtcg gcatcaaaaa ctacctggcc acaagcagca
     3061 ccaggagagg caggaagacc tttggctaaa acagtcattt tttctttgga ctgggggtcg
     3121 agagtgggat gtaaaagctg gtcgagtttg tcggcctcaa gacgtaaaat agcttctttt
     3181 tcggtgatta gtttctctcg gacttgatcg acggcaattt tgatagcagc gtgagcagta
     3241 cgtttgccgt ttcttgtttg gagaatatag agaacaccat tttcaatagt gaactcgacg
     3301 tcttgcatat cgtgatagtg attttcaaga ttttgaaaaa tttgaagtaa ttgggaatga
     3361 ataatttcgt tgtcttcttt aagagaatcg agagttttgg gggtacggat accggcaacg
     3421 acatcttcac cttgggcgtt cataagatac tctccataag gtaggttttc gccagtagaa
     3481 ggattgcgag taaaggcgac accagtacct gagtcggcac caagattacc aaagaccatg
     3541 gactggacat tgacagcggt gccaaaaact ttggattcgt cgatacgatt tagacggcga
     3601 taggtaatgg cgcgggtgtt gttccaagaa ccgataacag caccaacagc tccccaaagt
     3661 tgctcaaggg catcggtagg aaattcacga cctttttcta tcttaaatag ttctttgtag
     3721 cgtttgacta cttcttgcca atcggcggcg gtcatgtcgg tgtcggcatg aataccccga
     3781 gattttttga cttcttcgat gtgaaattcg aatttttgat gatcaatgtt ctcgacgaca
     3841 ttgctataca tctggacaaa acgacgataa gagtcccaaa caaaacgagg gttgccagtg
     3901 atctgttcta gacttttggc aacttcgtca tttagaccga gattgagaat tgtgtccatc
     3961 ataccgggca tggaagaagc agcaccagaa cggacagaga ctaatagagg gttctttaaa
     4021 tcaccgaatt ttttacctgt aattttttca agattggcta aatgggtagc gacttcggac
     4081 ttgagagtat ctggatattg attgttatta agataaaaat aacggcaaac ttcggtacca
     4141 atggtaaaac caggagggac aggaatgccg agcttgctca tctcggcgag gttggcgcct
     4201 ttgccaccta aaatgtattt catcgaggca tcaccctcgg taatgtcctg accaaagaaa
     4261 tatacaaatt ttgtcatatg aaagaaggta tatcagaaaa atgcaaaaaa gtctaaatcc
     4321 taaattgtaa aaaatctagt ttctttcgac tttgatggtg cactcggtaa ccagggcggc
     4381 gagagtgccg acaacggcaa gggcagggac aagaactgca ccgacgacac cgatggttaa
     4441 aggtaaaact acaagagttt tatcgttttt atcgatgata ctgatttgac gaatattacc
     4501 ttcgttaatt agggctttaa ctttttttaa taagtcgtca cctttgactt caaaagtatc
     4561 tctgggttgt ttggtggtca taagagaatt ataacctatt ttcaatctcc ccctaccccc
     4621 tctttgacaa gaggggaaac atctaatgac tggttagata ttgattgtag ttttcattct
     4681 tgtcaatttc ttgacgaaga gcatcacgaa taatttgagt gcgactttta ccagtagtaa
     4741 attgagccca atcgagataa cgtctttcca aattatttag aaccaaatga aaacgatttt
     4801 tctcatcaat gtcggaatat ttttttatga aagtatcgat ggttgagtca agttgtttta
     4861 aattgtattc ggagacttga agaatatccg actcaatgcc gtgaataaac atctgggaaa
     4921 attgacgatg tttcttgcct tgccagagga caagggtagg tttgtgatat tgaagagcaa
     4981 cggtgatctg gtgccccgta ctaaagttgg aaacagtgtc ttcgataatg gccaagtcgg
     5041 cttctttgat ggcggtgatg ttgtcacgat agattttctt aatatcacga atgacagtgt
     5101 ccccgtcgtg taataatttg gcggtatgag gcaaccagtc gcgagtgagt aaatgacctt
     5161 gggaaattaa atggtttcgg atttgaaaat aagttttttc atgagtaaag aaatcaccag
     5221 tggaacaagt aaaatagatt ttcatggctg agtgtagcct atgtgctaat tatgtgcaac
     5281 atgtgcatat acaaaaatag aggttaatta tataaatggt tcatgaacaa attaactttc
     5341 acccgaatgg tggtcggcta cataattaaa aatggccaag tgttactggg gctacgaaaa
     5401 aaagtaagtt ggggactagg cgaaaactta gtgtcaggaa ttggaggcaa aattggtgat
     5461 atacccggat tggaaaatga aagtaacgaa gaagctttaa tacgtgaatt tgaagaagaa
     5521 attggtgtca caccaataaa ttttaagcat atgggtaagg tgacatttgt atttccaaac
     5581 aaatcaaaat ggaatcagat agtcgagata tacacaattt tggaatggct aggtgaacct
     5641 actgaaacag aagtgattaa accgaaatgg tatgacaaag ataagctacc actcgagctg
     5701 atgtgggatg ataatcgact atgggtgcca caagtgataa atggagaaaa gatcaatcta
     5761 gaggtgatat atagcgagga taacaagata gtaaaagaag ttgttaaaat tagccatgat
     5821 gaaaaaaaat cgaccgcaga ggttacctta tgaagaattt ataaaaagtt ttgaaaaagt
     5881 acccagacta gcgattaatt tattaataac cgacaaagaa aatagcgtac tactaaccag
     5941 aagaaatatt gaaccatcga agggttgttg gcattttccg ggaagttatc tgctaaaaaa
     6001 tgaattaatt gtggaggcgc aaaaaagaat tgctgaggat gaattgggat taatattgaa
     6061 acaaaataaa ataaagttat tgggagtgtt tgaggacttg aacggtgacc caagaggaca
     6121 tgtgatcgat gtgatgtatg gttttaaaat agataataaa acaaaagtta agccgactaa
     6181 agaaactttg gaaattaaat tttttgataa attacctgac gacattggtt ttaatcatcg
     6241 agagactttg aagaaattag ggtttagata aggccattgt ctttgagaag atcgtgaatt
     6301 ttgctagcga caagtgtggc attagcttta ggaggaaggt ggagaaaaag gtgctgagtg
     6361 tcgagatttt tctgatttat atgaagttgg gtacggtaag caatccaatt gcaattgtaa
     6421 gtgcccatgt tggtaccgat ttgaaaataa tttgagtcat aattatcgac aggaggcata
     6481 tttaaatcta gcagtatcgg agaaaatgga taaatctctt tgtcgttgta ggcgtttttg
     6541 gcttgagtct caattttaat tttgcttaaa ttacgaagac cgtcaccaag accgatgatt
     6601 aaatcgtagc gattattttc aatgtattta tgaaaaaagt tttgaggaaa aaagaaaaca
     6661 ggcagaaaag tgatgccttc ttcggggagt agtttttgca attcagaaag ggtgcggcga
     6721 ctaacatttg tcccccagtg attgctaaag gcgtagatga gagtgggcat gttatctttt
     6781 tcctgttaaa cgaagggtgg ataactgacg ggaatactgc tctgataaag cttgattaag
     6841 cagactactt ggatcgtact ttctacctgg attaaaaatt tttcctctaa ggtatagaag
     6901 gtaacctgcg gtaacatcat cttcactcct atcgtcagaa aacttgtgtt gatatctaac
     6961 tttttcgcct gcagcaatgc gaggattaag agacgggtcg ctagtaagag tttgggtaag
     7021 gttgattgtc ggaagggcga aacgaagcaa ttgaaatata tgcggaggtg ctgaacgact
     7081 accaactcca ggaagatcga gaatataata gcgatgaaga ttgtctggct gacgacactc
     7141 ttctacaagg ttactaagct cttgacgcaa ggtaataccg ctggtgtcgg ggacttcgtg
     7201 ggcagctctc ttccgacggt aaacgtcaaa aatagcctta attatcttca agggagattt
     7261 caaatagcta ggatcctgat cgatgattag ttttaattcc gagtaacagt tgttgattga
     7321 ctcgattccg aacatatcaa aatcccgaac accgataatg gagtcgtcat ttgagagagc
     7381 aacagataag agtgcagcag tcttgccgct ccaaggagag ccctcaatac ctaaaatcgg
     7441 aataggatat ctattgtcag gaaagtcgtc aagattgggt cgagtttcca tattaaaagt
     7501 ggcatgaaca gcaagtggaa cagaaagctt ggtaagggct tgtcgaaaat acaaactagt
     7561 ctcttcgtca agagacactt cactaaaact ttcaacacct ctatcttgga ggtcccgagg
     7621 aacaccttgg ccaacaataa tgtcgtctcg atgttgaggt aggcacataa tgctaccgtc
     7681 tagatctgga acggataaaa ccttgaatgt ctctccattt tcaccgtgcc aagtgtgaac
     7741 ttcaccagct actctgggac gccaagtttc tggagtttta acaacaggga gagagttttc
     7801 tctgaccgta gctgtgtctg aaaacgccat gttattttaa ttcaaaccca actttgggta
     7861 tggtggtgat ggtaaattta tgtaactttt ggcttagacg tcgcatctgc ttattgattg
     7921 cttgatctga gaactcatca tattttccac taccccactt aatatcggag atatcttctt
     7981 tggatatctt gccatcattc tcgagcattt tgtggattat tttagactct aaaggtgtta
     8041 acttctcttt tgatttggca cctttttctt caaaagacag gtcgaaatta accttgatgt
     8101 ctaaaacgtt tttaatatat tttcccaata aacctgataa gtattcaccg tcttcattga
     8161 caagcgaaag tagtttaagc tcggacggat catacccaga ctgagaagtc acgatgttgt
     8221 ccataaggac ctgaaatgag ttatcttttt cgtctatcaa accgtcatga acaatcagat
     8281 atttagctaa cttgggaata ccaccagaca gtctcatgat tagattagta aattctgggt
     8341 cgggctgacg tccggagcga gaaatcatat cggagatgtc atcaacagac cctgacctaa
     8401 ggggaaaact aatatacaga tgatctcgaa tgtctttgaa cagattagag tgatagtcgt
     8461 ggccagaact aatgacgatt agatattttt gacgaacatc gactgactta acgtaagaaa
     8521 gtgcctcttc gaaaggtaaa tcgataaggt tatacgcacc gaggatggca tttggtttat
     8581 ttatataggt tagtgactgg tcgcctgtcg aaatttcttt caaatagtga gtacaaccac
     8641 ttccggcaaa agtaaaaact accatattac tccctttttg cttggctagt tcgatttttt
     8701 ctcgcaaatc caaaaagata tctgaaacat attgatccat attcttaata taccacggct
     8761 ttgggaaaag atttttgtcc ttcatttgtc cgatgaatgt cgggaatata agtaaagatt
     8821 ataagataat aaccttaact tagctggaag acaatgacaa agcgttgttg aacttttaac
     8881 taggtgcaca ttaaaaaata ggttttaggg aaaaaaacgg ttaggggttg aaataagcct
     8941 gtgaggcttt gacaacccct accctacccg tccaaaggag gacacacatg aataccctga
     9001 gcaagagact gctgtttgtg gcgttagcca ttgttttttt gtccgcttgt aaccaggtcg
     9061 ctcccgcgac cacggccaac ttgccgactc caaccatcac tctgacttca accgcccaac
     9121 caaccgcgca gccaaaaagc actgaagcct cgactgaagc atttttcagt tcgatgggat
     9181 ttggccgcat gccatgggga ataagcttgt ctggtgacaa caaagtctgc ttcactgagt
     9241 tacggtctaa tccccgtgct tgtgatggca atactttgaa aatcactgcc aagagcccct
     9301 tgctggttct caagaatggt gaaattttcc tatggctcaa agtcaacgac gctataacga
     9361 tgcaatttga tcgtgatacg tcctacgact tctacggcct ggctgacagg aaatacccgt
     9421 tctatggtga gtggaggtcc acgatgttta tgtggagtga ttcatcaaag acgtttccga
     9481 tattgatgct accccaaaat gagggaaatg accagtttgt cgaccttgcc agtgggataa
     9541 tctatgattt tcctgctaat agcgagattt ttgactatac tgtttcttcc ccgattttcg
     9601 gacatcttta ttgcaacgat atctgcgtct ttaatcccta aacctattca ccgagccacg
     9661 ctcaacacga ttcagacctt gtgtctgttt tgttgttgag tgtggttcgg aaaggttttc
     9721 cagcacagaa agaaatttct ggattggagt cgcctttatt ggcgcagata gcccaacggt
     9781 gcgttggaga ctatcagcac tttcaaaatt tgtgtgaaat aaaaaggaga atggaaatga
     9841 atacgaaaat ggcatctgta atagtattgg gcttgatcgt cttagcagct ttaattttca
     9901 tcgtaggagc aattatgaca ccaccggaag ttgttgaaac accggagctg gtggaagccg
     9961 agacaaggtt cgaaagtgga accattgagg tggccaccat cgcttacctc gacgacgagc
    10021 atgccttgag ccaattaccg tgggttgaga atgccctcta caaagccttt gtgtttggcg
    10081 agtcagccac gtatgtaacg aaggaaattg aaggcgataa agtctttatc cacaatgtgg
    10141 tcgttatcaa tatcgatgta caaggatatc gatccacctt ggccttgaaa atggccagag
    10201 atgccggggt tattgtatat gtcccttgta atgagtggac acttgaaatg accaaggaac
    10261 atgtgtgcac tgaaatacca aacgatttgt tgagcgacta tatgaaaaaa gctgtcgatg
    10321 gcggtgcatt cacgctgggt tgggtggaat ttcaaaggtt caaaccaaca agtgagctga
    10381 ccagcgaaat tggtttcaaa gcattggtct attacagcgt gaactacgtt aagcgtttcc
    10441 gttaatttca cacaaaccac cttcgctgtc taccctccct ggcgagagcc agggagggac
    10501 ccttagggga aaaagatagt gaaggtggaa ataatttaaa acaaaaaatt aaaaattaat
    10561 taaaaaaaat gaaagagaga ataacagtag gaaaaatagt tgagaagaca gtctttgcca
    10621 gaggtaatat cgtacccgta tgggaaggtt taccatttac tagcaatgta tcgaaaaaag
    10681 gtgaattggt agttattgag gattggatag caaaaagcat gtttgatttg tgtacgagag
    10741 ctatgcaagc agatcaagaa ggggcagcta agatgtttga gaaacttttc tggtccgaaa
    10801 gtattaattt accagttaaa gaaaaagcac tggctgacat ggcaagaatt gccaatgaaa
    10861 ctggagagaa aatgcaagaa gatggactag aaatggttaa aagaattgat gaagttatta
    10921 atggaggtca tatactagat cgagctagcc agagatttgg agacaaaata ggttatgcaa
    10981 tttctgtaat gtctgatgaa aaaccaattc gaggtagagt taacggacag ttagcttcgg
    11041 tagttgattt tgatatcgta atgagggaga gaaggaaagc tatttcttct caatattaaa
    11101 aagactcgac tttgctgtca gtgcaccggg ggcacggagg gtgaaaatgt ttttaaaggt
    11161 actgccatca aaagtgaaat cacccttgtc ggtactaata cgaactgact tggtaactcc
    11221 tgaagtagag tagtcgacgt tgaccgagtt gactgatgaa acagggccac ccttgtcccc
    11281 tacttgacgg cgtaattcat cacgagacca ggcggaacta tcgcaagatc cgagacaatt
    11341 ctgaatctga gagagatgac tttgatcgtt ggttttggaa aaataaagaa cggcattaac
    11401 gatgtcagaa aattcttcgt tatttaacca cggatgagaa cggccagcgg aaacattgga
    11461 gcgggtcttg taccaacctt tgtaatacca agaagatccg gcttgcctct cataggcatc
    11521 gttgggccag caagattgat taccgcaagt ggtatcccag agttgaggtg tgctatgccc
    11581 agcagtagag taagaataag tgacaccacc cgaggttgaa gcatacatgg tagaaaatat
    11641 tttaccggtc tgattacttt taaccaccat accgcgagta tcttcaactg cctgtttcca
    11701 gcgaccagga gaattgagac gagactggct ataaacctga caggcctcag tgacacaaat
    11761 ggtacccgat aaattattgg tagagttaag ggcataggta cgagcggcaa tggcttgagc
    11821 tttgagggct tcgtatccac ccttatcacc ccaagaagcg ggcatctcag caatcccgat
    11881 aagataattt tcttcaaaag gaagggtgcc gacagtggtt tttatagaac cgccaacatc
    11941 aactttctcg acttttatat ctccgtaata ggcttttaga atctgctcag cagattgccc
    12001 agctttggaa cgaccataag ctccatactg agacataccc ttctgatgag gagccccaaa
    12061 agaaaagaca gcaaaagctg gtgagaaacc aggattgtag tcggaacgag aggcaggatc
    12121 atcacctgag gaaacgtcgc caacagaggt gttaaacatg gcagttttct cagcaattaa
    12181 attttgctgt tttttggtca actcggcttt gtaagtttcg gcttttttta tctctcctgc
    12241 aaggaagcca aagcgagttt caatactttt tttaaatgcc gataatttgg ttttttctga
    12301 ctctagggat tgtttatttt gattgagatg ggttatctca ttcgaataac taagaatagt
    12361 tgacctgtct tggttaatga ttgattgata ccaagagtac tgacgaagaa gatcggatcc
    12421 ttctgaacta gtaaagaata cactgaaagg actgaacttt ttggtattga tgtaataacg
    12481 tctaaccctc tccccaagga gagtctgttg aacttcaagg tcagcctcct tatctataag
    12541 tcgaaggcca agatcagaga cttggttttc aattttgact atagtggctt tggctgaaga
    12601 aatcttacta gaaaggtctg tagattcttt tttgagcgga gcaatagatt tttcaaggga
    12661 ggatatttga gaggagagct cggctatttc ttcgtcaaga gattttgcat aaatccccct
    12721 cggtccccct ttaacaaggg ggaggaaaac taaaagtatg gatagtgtaa agaggaaaat
    12781 ttttagcaca aataattcta ccatcatggt atacttcagt tatgattttg gcgaaaattg
    12841 cgaaggtagt gataattgtt gctatgcttt ggtcgagtgc aatgacacca gcgttggcgg
    12901 tgaatgcttt ttgtcaaaaa gggtcagacc cacaagttaa tactgccttg ggttgtgtac
    12961 ctgtaaaagt ggattctttt gtaatatggt tgatgccacc tctttttgga atagctggag
    13021 gaatatcatt cttactaatg gtttacggtt ttttcttgat gtctacttct tcaggtgacc
    13081 caaaagcagt ccagggggca tgggagacga taacttcagc aatatctgga ctgttggtat
    13141 cgatattttc tatattctta ctaagattaa ttacggttgg tatattaaaa attccaggaa
    13201 ttaactaaaa tggcagtaaa ccttccaaca gatccaatga gtgcgaagtt ttctagtgta
    13261 ggatcgataa tttctgcctt gttgccttat gctatggtgt tggcaggatt attgatgttg
    13321 ataatgttgg tgtggggagg aataactttg atgacggccg caggtgatcc aggaaaatca
    13381 aaagaaggtt acggtaaaat tcaagcaggg ataattggtt ttgtgattgt gtttgtgtct
    13441 tattttgtgg cacagatagt gcaggtagtg ctcggggtaa agtttttgca ataataactc
    13501 tgatatattt ttgaattagg tggtatacct tcatatctct aatctatcaa atgagtatgt
    13561 aaaagatggt tgcccgaggg aagatcataa cttttttgtt cctgtgagag aggctaggcc
    13621 gtgcggagga atttctgatt caaattgtct tgcctgtata gattgcaagg tgagaattcc
    13681 tatcaccggc agggttgttc caaatggtaa agagacgaag agagtgttct tatggacgag
    13741 ataggcctat tttcccatgc gacggtcacg ctgttgatac caagcaatag ctttttcaaa
    13801 ttcttgagga gtaaagtcgg gaaaaagaac tggcacaaag agaagctctg agtaaactga
    13861 ttgccaaggt aaataaccag aagttctttg ttcccctcct gttcggatga taagatcggg
    13921 gtcgggtaaa ccagcagtat cgagatgtgc agaaaaaagc tcatcagtta tagactctgg
    13981 atcaatcttt tctgaaatga tttttttaac ggctcgaaga atctcatcgc gaccaccata
    14041 gttgagagcg aaagtaacat taattttgtt gttggaggaa gacatagaca tcatttcaag
    14101 cgtttttttg gcgatgtctg aaggaaactt gttgatgtca cctaaaattc gtaaacgagc
    14161 acccttttga ataaacctag cagccaaagt acccaaccct aggcgaaata catcgaatag
    14221 tcctgtaagc tcaacttcat cacgtttcca gttctcggtc gagaatgccc agaaagtgac
    14281 atattctata cccagttcgc cacatttttc gattagaggt tcaattactt ttattgtcgc
    14341 ttgttcatga cccttaactg ggtctaatcc acgagcccta gcccaacgac gattaccatc
    14401 cataataatg gcgatgtgtt taggtaactt attaggatca attgaagtgt caatagactg
    14461 taaggccatg atgatttagt cgaaaaaatt taacatatca tttgatttac ttttttgtat
    14521 gatacaggtc ttctaatatt ggatagaggc cttccatgag gtcttttctt ttatatagct
    14581 ctactggtgt ttcccactta taacttacgt taccagccaa gtcaagtttt ggcattactt
    14641 cgccctcaga aaccttttgc atgaaaacag agaaatatat ctccatacca gaacgaatcc
    14701 agtgaaattc ttttttaaac ataagtttgt ctttgtcaat taaatagttt atttctgcaa
    14761 aaactttttt tatgacagca tcttttggac tcaaaccatc gtcgacgcca ccgcctgtca
    14821 aaccccaagt tgatccttct ggcacatctt tatttctttt taaaaccaat atttctccgt
    14881 ttttattttc aaaaataact ccagcagcgc tgataatatt atccattgcg caattatat
//
LOCUS       DDWP01000098            3457 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_64680, whole genome shotgun sequence.
ACCESSION   DDWP01000098 DDWP01000000
VERSION     DDWP01000098.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_64680"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1212 a          503 c          797 g          945 t
ORIGIN      
        1 taacttgctg acgaatctcc tcaaggccat tattgaactg attgtaggga gcgatgacga
       61 gaaaccagtc taagataaaa aaagcagaga caaatgcaag aagaagtaat ttggattttt
      121 caggtttgaa catgtgcaaa ttatacaccg attaaacaat aaattgttta gtcccttctt
      181 ttttgagcca gagattgtgg gccttggtgt taggatcata gatagtcaat tggtcccaga
      241 attttttgga atggtctttg atcttgagat ggtttagttc gtgcaaaaga atatgcgtaa
      301 atacagctcg aggaaacaag attatttgcc aattgaagtt taaattgcca cgataggaac
      361 aagagccaaa acgggaggtg gtatttctaa cactgacacg tccatattca aatccataaa
      421 gaattgacaa acgtgacaac tcatctttaa taagagaaag agctaaagga cgaaatttgt
      481 gatcaataat cttttttaaa tgcgaaatag atagtgaagt cgtgtttgca taaattttgt
      541 gttcttcttt gaagataaca accgaatcca tgggcatctt tttgacaata aaattgtagt
      601 cttcgcctaa aatttgtaga gttttaatgt ctttaatggt ctttttgata gttatcttct
      661 gagaattttt aatgatccac tcctgatgat cattgatgaa tttggtaatg acaaaatttg
      721 gagtaagatg gtgacaacta attacaaaag actcgggaga ctttaatctg aggcgaattg
      781 aaacaatcga ctttcgttcg atttcatagt taaatgagtt ccctttgagc aagatacttg
      841 gcataggtgg taatatattg tagatgattt atcttggagc ggatcatagg ggctttagac
      901 taaaagaaga aataagtaag tggcttgagg aaaaccacta tgaatttagg gatttaggtg
      961 caaaaacata cgataaagat gacgactatc cagatatttc tataaaagtc tcggagaaag
     1021 cagtagcaga aggggctaaa gggatattgg tttgtggttc aggagctgga gcctcagtaa
     1081 tagccaataa aataaaaggg gctaaagcag gattgtgtac gtctgaaaaa cagacgaaag
     1141 cagctcgaaa tgacgatgac atcaacatac tatgcttatc ggcagactca gtaagcgtcg
     1201 aagataatat aaaaattgtg tctgtttttc tagcgacgat gttttcttca gaagaaagac
     1261 atataagaag gcttaataaa attcgtgact atgaacttaa aatcagttag agatttatta
     1321 ccaggaacaa gggtcgtact gagaatggac ctagatgtgg ccgacggtga taattcgaga
     1381 ttagaaaaat caatcccaac aataaaattg ctgttagaga aaaagtgtcg ggtggtaatt
     1441 attggacaca aaggacgtcc tgaaggcaaa gatgaagaat attccttaag atcagtatat
     1501 cttgatttaa tgagtatgct tgaaccaagc ggagaaaatc taattgagag tgtgtttatt
     1561 gatgatatta gtaataaaga aaaaattgat caagcattgg taacaaatca gatagttttc
     1621 ttggaaaacc tacggttttg gaaaggtgaa gaaagcaatg acccagactt tttaaaaggg
     1681 ctgatcgaaa taactcagtt ttatgttaac gatgccatat cagtagccca ccgcaaacat
     1741 cggtcgataa tgctttacaa gaacttgcca ggtttttatg gattggcatt tattgacgaa
     1801 gtagagaaaa tattaaaagt cacagaggat ccaaaaagac cgctgactgt aattcttggt
     1861 ggagcgaaag aagataaatt gaagtattta gaagaattag agaaaattgc tgataatgtg
     1921 ttaattgggg ggaaactccc acaattaata ggagaaaaca acgtagtagt tgccaagctt
     1981 agagaagatg gatttgattt aagtgatgag gatattgaaa actttaaaaa aataattgct
     2041 gagtcgggga tgattgtgtg ggcgggggcg atgggatggt ttgaaaaaac ggattgccag
     2101 aaaggtacgc tggaaattgc taaagctgta gccgagtccc ctgcttataa gataattgct
     2161 ggaggagata ctggagcttc gataaagaac ttaggattgt tggacaaaat tgacctaatt
     2221 gcttcgggcg gcgggatgat gttggagtta ctagccaaag ggaggttgcc agcatgggaa
     2281 tagacaagcc atccatgtat atattcagtg ttgaacaaga aagactacag aaaacctttg
     2341 agtttcttga tgcttcggga catgtagagt tggtaattag accttcacat attgactatc
     2401 gagaacaaag gcaaaaagca aatgtgggct ttatggaaag aaaaggtgta tttgcgtcct
     2461 ttactgaaat ctcatatgcg tcacgatctg aattaattga actaattcga gacatctctc
     2521 ctagaattcg tacagtaaga acccttgttc ctgatacaaa aaaagatcta gtcatatatt
     2581 gatatatcta acaaacacgc tgaaatctaa aatactagaa ttttatatca ctagaacgat
     2641 agtatagaat gaagtatgga aaataaaaaa atacggattg ggattaatgg atttggaaga
     2701 attggaactg tagtcctgag ggcaatactt cttgaaaaat atgacgtaga agtggtggca
     2761 ataaatacca aaggtaacgt agagatggaa aatttggtaa tgcaatttaa atacgattcg
     2821 gtctatggtc gggccccaat gcaaataagt gccggtgaac cacaaaaagg tggggagata
     2881 ggtagattag tagtaggtaa ccaatcaatt cctatgctgg gagaaccaga tattagtaag
     2941 atcagctgga aaaattatga tgttgatgta gtacttgaat gcacaggtgc atttaacgac
     3001 aacaaggcct tggcgcatat caaaaatggt gccaaaaaag ttgtcctgtc ggcaccggca
     3061 aaagacaaaa gtatcccaac ttttatccta ggagtaaacg agagtaggta taaaaatgaa
     3121 gatctagtat caaatggatc ttgtactacc aactgcgtag caccaattgt taaattaata
     3181 gatgaagcga tcggctttca agaaggagtg ctaacgacga ttcatgctta tacgactaac
     3241 caaaatatag ttgatgggtc gggtaaagac ccgaggagag ctcgggcagc agcgataaac
     3301 atagtaccga cgtcgactgg agctgctgag gcagtgattg cggcttatcc agaagcagca
     3361 ggaagatttg ctggtacagc aattagggta ccagtaattt gtggatcata ttctgactta
     3421 acatttaaac tagtgcgtaa aagcgatatc gaagaaa
//
LOCUS       DDWP01000099           12868 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_26685, whole genome shotgun sequence.
ACCESSION   DDWP01000099 DDWP01000000
VERSION     DDWP01000099.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12868)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_26685"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3868 a         2593 c         2445 g         3962 t
ORIGIN      
        1 gttgatggga caattgccaa agcagtcact cttaaattca aaggtgctat cattttaatc
       61 cgtcctggta aacaaggttt cggcttgatt gctggcggtc ctgttaggtc agtcgttgaa
      121 ttagccggta taaaagatat cgttagtaaa ataatcggat ccaaaacaaa atctattaat
      181 gtctgggcca ctattaaggc acttaccctt ttacagaaga actaaatatg gatatcttat
      241 ctcaattacc tcaaacaaca gaaaaacaac aaagaagagt tggccgtggt tacggatcac
      301 aaaaaggtgg ccatactact ggtcgtggtg ctaaaggtga caaagtccgc ggtaagacta
      361 ggattacttt cgacggtacc aagattaaga aaggttggat taagcgtacc ccttttctta
      421 gaggtaaaca ccgagttctt gctgctccaa ttttttacac ctacagtctc agccagatgg
      481 acaaatggtt tgagtcaggt gccaccattg acatcaaatc aattgcttta aaatcaggta
      541 ttgacgagaa agtattactt aatagtgtca agattttggc ctctggagaa acgtctaaaa
      601 cattcaattt caaaggtatt cttttctcta aaagtgccca ggagaaaata ttgtctctag
      661 gtggtaagat agagtaatga accagctcgt caaatctctt tccctttacg tagaaggtct
      721 aaaaacacct cttcttaggc aaaaaatagg tataaccatt ggtgtactta tccttttccg
      781 ttttattgct catatcccag tacccggtgt cgatcttgcc ttacttcgac agttttttag
      841 tagcaaccaa ctcttatctt tactcgatgt attttccggt ggtactctcg ccaatttttc
      901 tattgccgct ctagggttaa acccctatat caacgcttct attatgatgc aacttcttac
      961 cttggttagt cctaagcttg aagagctgag taaggaaggc cagtatggtc gagagaaaat
     1021 aaatcaatat actaggatcg tcaccatccc tctctccatt gtccagtcgc taggtatgta
     1081 cggtattctt agatcccaga atatcattgg tgagctagac atattgaccc taattgccct
     1141 tgtttcaagc atgttagccg gcactatgat tatgatcttt ttaggtcaga tgattaatca
     1201 gtttggtgtt ggtaatggta tttccatgat aatctttgcc ggcattattg ctcgcttacc
     1261 cttatctgct atccaagctg ttagcttagc agattttagt aatcctcaga ctactcttaa
     1321 tttagctatt tttgctgtca tggctattgc tatggtttta gctattgtta ccgttgaaga
     1381 agcagttttg cgagttccta tacattatgc caagcgtgct cagacgacct atctacctat
     1441 caaggtcaac actgctggtg ttatgcctat tatctttgct gtttccttag ctactattcc
     1501 atcacttctt ggtcagtatc ttggcaaagt caacaacact gttgtggccg atcttgctct
     1561 ctctgttgcc cgagtcttta atcctagtgg ttatgcctac attttgtttt attttctcct
     1621 ggtcattgga tttacatttt tttatacatc tgtcgttttc aaaccaaaag aagttgctga
     1681 tgaattaaga aaatcaggtg gttttatccc cggtattcga cccggtgttt ccactgagaa
     1741 ccgactcaga tatctcctta accgtattgt tgctattgga gcagttttcc tgggtgtcat
     1801 cgccgtcgct ccatcaattg tctccaacat taccaatctg actactctga ctatcggagg
     1861 tacttcagtt ctgatcgttg tctctgtggt tgttgagctc actcgcaaga tcgagaacgt
     1921 cgtccagacc tacaactacg ataaattaac ttattaaacc cctctggtcc ggtggacacc
     1981 ggaccatctc cccttcaaaa ggggagtctt tgcatgtttc tatttcgact taacaatttt
     2041 ttttgaaacg gcatcaataa acaacctaaa tgcaggtcta ttctctttct cataaatata
     2101 gttgacacct agtaattgtt tacacttttg acaagttgtt gtaaaagttg gatcaaagat
     2161 tctgtccgta tacattcgtt ttagtatgcc tggtccatct ttctgataaa aacatagatg
     2221 ttggccacat ttagcacagg aaatgttcaa gagtctagaa tatccccctc gggctttttt
     2281 atatttatca ctttttaatt taatctctac catagcctaa ttttatcaaa ttcaaaaatg
     2341 aagtatcatg tagataattt ttctgatgaa gggcttcgga gtccacttta tgtggagaaa
     2401 gttcagtttt gcccaacaaa aggtttataa atattgccct tttgaaggga aatgtcccga
     2461 gcttgtcgag ggacaaaggg tttaaataaa aatggtttaa aatggtgtat gcatctcgat
     2521 gaaaactata ttactcgtcg tggtcacaac aaatatctct tagagttacg agaactatct
     2581 agaaagttga gaaataacca gaccattgct gagaagaaga tctggtatca attactttgt
     2641 agaaaatatt tagattttaa gtttcttcgt caaaaaccaa tcgatcaatt tattctagat
     2701 ttttattgcc ctaaattact tttagatatt gagatagatg gtgaatcaca cattaaacgt
     2761 caaaatcatg acaacttcag agactatcga cttggtgttc taggaatcaa aactatacgt
     2821 tatactaatg aagaagtcat caatgacttc tctaacgtag ttaagcatgt aaaatcacaa
     2881 atcgaagtca gatgtcaaga aatataaaaa ttaattgccc ttttgaaggg aaatgtcccg
     2941 agcttgtcga gggacaaagg gtttaaatgt taaatctctt tttaatcggt ccgtctggtt
     3001 ctggtaaaac tactcaggct gaaaaagttg cccaaaaata cggcttaacc catttttcta
     3061 tgggccagct tcttcgtgat gaaattgctt ctggcagtgg tttaggtttt gaagccaaaa
     3121 gttatgtcga tcaaggtatt tgggcaccag acgatcttat ttttgacatc ttaattaaca
     3181 agcttaaaag tctcaatttt cagaacttca tcatcgatgg ttttcctcgg gttcttaatc
     3241 aaggtcgcgt tatcgagttt tatttgcgca aacttaatca acccgtatct ctgctaattc
     3301 acctcaatgt aactttccaa gaaatcatgg ataggcgagc tctactaggg gatgactttc
     3361 aagaaaaaga ccgcaccgac aatacccctg aagatattgc tcaccgtcaa caatcctatg
     3421 aagaaactat tgacccgatt aaggattact ttaaaagaca cggtaaatta gtcgagatta
     3481 atggcaaccg ccccgtcgag ccaatttttg aagatatagt taaagagatt gacaaattaa
     3541 atgcttaaaa ttactccaga aataaaacag agattcgaga cttcggtagt tgctttaggt
     3601 acagttagtc taactggtgt tcctaacgtc gttgctgttg cttgttgtaa agttgtctcc
     3661 ggcaaccaag ttctaattac cgacaatttt tttaacaaga ccagggtcaa cttgaatact
     3721 aatcaatatg tttctttgtc tttttggaat ccggtagatg gtgataacaa ctggggttat
     3781 caatttaaag gtactgctga ggtttttacc gaaggtaaat ggaaaaatat cgttaataac
     3841 gatcccgata atattggtct tgcccatccc ataaggccgc tattttggtg actattacag
     3901 aaatttggga tcttgctaac cccaaactga tatcttccca aatttagatt acattttcct
     3961 tgcattttcc cttataatac aattcatcaa atatttatat tcatctccct tgtcaaaggg
     4021 agaagctccg gtgaacaccg gagagaggga ttgaaaacat taatagttta atctaaatga
     4081 aagatccaac tgtccgtggt accgttactg aagttctacc aaaccagctt ttccgtgtcg
     4141 agcttgaagg cggtaaagtg atcatttgtc atttagctgg taagttgcgc attaatcata
     4201 tcagatgtct tgctggagac accgtctctt tagtcctttc tcctgaaggt gaaaagggtc
     4261 gtatcgtcta ccgaggatag acaaagaaac ctcccttttg aagggaggat gcccgaaggg
     4321 caggtgggtt tattccactt aaattatctc tttgatattg ctacaatttt aggttacaat
     4381 atccaagtct ttagtactca aacctatgaa agtaaaatct agtatcaaac gtcgctgcaa
     4441 aaactgtaaa gttgttatcc gtaggggagt gcgacgtatt atttgttcta ccaaaaaaca
     4501 cacccagaaa cagggataat tctatgagaa tctccggagt cgaaatccca gataatgaaa
     4561 gagcagaagt tgctctaacc cgtttttacg gaattggccc agtcaatgtt gttgcattgg
     4621 ctaaaatgtc caagatcgat ctaaacactc gtattaaaga tctttctcgt gatgacatcg
     4681 gtgccctcat gaaagccctc gaaaattttg ccacagaagg tgaccttaag aaagaagtcc
     4741 gcgaaaatat tgatcgtctc cgtgccatca aatgttatcg tggtattcgt cacatcgttg
     4801 gtcttcctgt tcgtggtcaa cgtaccaaat caaacgcacg tactcgtaag ggtaagaagc
     4861 gaacagttgg ttcacttact aaggaagcct gggcaaagat tgaaacccaa caaaaagctg
     4921 cttcaattgc tactaacaaa taaactttat ggcaaacact acccccaaac aaactaacac
     4981 caaaggctca aaaaccccag ttaagaaaaa ggttttcaaa aacattgctg atggtcgact
     5041 ttacgtcaag tcatctttta acaatacaat agtatccatc gccgacagta aaggtaatgt
     5101 tcttgcttgg tcaagttcag gacactgtgg ttttaagggt gctcgcaagt ccactcctta
     5161 tgccgcttca actgccctag aaaggacttt agaagaagcc aagaaacatg gtattaaaaa
     5221 gttggaagtt ttccttaaag gtccaggcgt tggtcgagat gctactttga gatttctccg
     5281 tagtaagagg gattttgatg ttgagcttat ctcagacatt actcctattc cccacaacgg
     5341 acccagacct ccaaagtctc gcagtgttta gtttttttaa attattaaat tattaaattc
     5401 ttaaatcttc aaattaaatg tcaagaataa ccctcgaatc aaaatgtcga ctttgtcgag
     5461 ccgccgggac caaacttttt cttaagggat ctcgatgtct ttccggcaag tgtccaatcg
     5521 agaaaaaagg tgccattcct ccaggtatgc acggtattaa gagagccaag aaacccaccg
     5581 attacggaat acagcttcgt gccaaacaaa aagccaagcg catctatggt gttcaagaaa
     5641 ctcagtttca caactactat ctcaaagcaa aaacccttcc tggcctagtt ggtgttaact
     5701 tggtgaatct tttggagaaa agattagaca atgttcttta tcgtgctggt ttatcattat
     5761 ctcgatcgaa tgctaagcaa ataattagtc acggtcacgt tctggttaat gacaagactt
     5821 taaacatctc ttcctacgtt atcaaaaagg gagatgttat ttctttagat accaaatcag
     5881 tttcaagtta tcttgacaat ctcatgatcg ccgataagga cttcaaaact cctgactggc
     5941 ttgacgttaa caaaaccaaa tattcagttt ccttgctttc ggagcccgat ttgtccgatg
     6001 ttagccaaga aattgatgtt aatttaataa ttgaatatta ttcccgctaa aaaggagaca
     6061 tatatgatgg tagataccag caagttcaat atcaaatcag ttaccaacga cggttcttac
     6121 ggtaagtttg agttatcccc tctaattggc ggatttggtc acactctcgg caacagtctt
     6181 cgtcgcattc ttcttattac tatccctggt gcagctatta ccaagattag aatagacggt
     6241 gctactcact tgttcaccac cttaccaggc gttaaagaag atttggtaga tgtctcactt
     6301 aaccttaaga aagttaaatt tacttattca aaagatgaac ccatcgttct ctcgttgaat
     6361 aaaaaaggtg tcggcccagt taccgctggt gatattgctg gtaatgacct ttgtaaagtc
     6421 agcaatcctg atcagataat tgccaccatt actgatgcca aaactactct tagtatggag
     6481 ttaaccatcg agagcggtat cggatacacc ctagccaaag agcaaaaatc agacgttatg
     6541 ggtgaaatca ttcttgatgc caccttcact ccagtcattc gaagcaacta tcaagtagaa
     6601 ccaactcgtc ttggtaaaaa aagcaactac gacaaattaa ttatggagat ctggaccgat
     6661 ggttcaattg atccattagt tgcccttaag actgccgcta aagatatgat aacttctctt
     6721 tctcaaattg ttgatcctaa agaatttgat gaagaggtaa ttactctggc tgcaaaccca
     6781 tcctcttctg gttcagatat ctctatcgaa gagatcgaat tacctctacg tgttaccaac
     6841 gcccttaaaa aatcaggtta ctctaccatc gacaatctcg tcaaagctgg tagatctgat
     6901 gttagtaagg ccaaaaacgt tggtgaaaaa tcattaaaga ttattgatgc ctggcttaaa
     6961 gaaaaaggct tcacttggaa ataaattatg aatcatcgca agtttggtat aaaattaggt
     7021 agaaaccgcc gcgaacgtga ggctcttttc aagtctctaa ctaggtccat ctttacccat
     7081 ggaactatcg agactaccag tgctaaggct cgatctgttg tttccattat cgaaaagctc
     7141 agtaaccata tcatcactaa agatattgtt atagcccaaa gagaactttc tacttatatt
     7201 caggacaaga atttggttag ccaaatctat gctgaattta agaaagtctt cgaaggtcag
     7261 acaagcaact ttaccaaaat gtggaaaacc aagattcgtc aaggtgacaa ctctcttatg
     7321 gttaagtttt ctttcgtcaa accttactct ctcaaaactg ttaaagttga agaaaccaaa
     7381 cctgaagtca aagaaaccaa gaccgtcgct aagaagccag ttactaagaa attaaagaaa
     7441 taatatgaaa gctaaaacta ctgttaccaa aggaaacacc atcgaacgtc aatggcattt
     7501 agttgatctt agtgatcaag ttcttggccg tgtttgtgtc aagttagcct ccatccttca
     7561 aggtaaagat aaagctgatt attcaccaaa cagagatgct ggtgactata ttatcgctat
     7621 taatgccagc aagatcagag ttactggtaa aaaacttaaa gataaaatct attatcacca
     7681 cactggttac gctggccatc ttcgtgaact aactcttgga caacttctcg aaaaagatcc
     7741 aaggcaagtt atttctcatg gagtcatggg tatgttacct aaaaacaaat tacgtgatcg
     7801 tcgcatgact cgattaaaaa tatttattga tggtaaccat ccttacaatg acaaaatcaa
     7861 atagtaaaac tctctatact tacgcagttg gacgtcgcaa atcttgtgtc gttaccctca
     7921 aactttttgc tggtaaagcc gatagtatgg tcaatggcaa actatttgcc gactactttc
     7981 caggtaccaa caatcaagtt ctatcccaaa agccttttat tgttaccgac actactggta
     8041 agttctacta cactgccaag ataattggtg gtggtaaagt tggtcaactt gccgctttaa
     8101 ccttagccat atcacgtgct cttaagaagc ttgatgaagc tacctacaag tcacctctcc
     8161 gcaatgctgg actattaact gtcgactctc gtgtccgtga acgtcgtatg gtcggtactg
     8221 gtggtaaagc tcgtcgccag aagcagtcac caaagcgtta agatcttttt taaaaaagac
     8281 atcggttcat tggttaaata catctcagat taaggccagt gtttttaaaa atgtcaaaga
     8341 gattagattt ggctgtggtc aaccatatat catcaaaaac ggataagcaa tcgtggtgaa
     8401 cctctattaa ttcaaatttt gtagcgttat aatatgtctt gtatgcgaac agcacaacag
     8461 attctcgaag ctcggggaaa agcagacgac ccaaaaggtg aacgatttgg tgtcacccca
     8521 cgatttcatg gcaggcaaga tattgcaatt gttgtcacac agttgtctgg tggtcgtctc
     8581 gtactagata acatgcaaac cagagtggtt ggccctatcc ttttatcaga aaaaacaggt
     8641 cgaagagtta tccatccaga ggatcccaca gtaattttcc aataacttac tgtagactat
     8701 caatatgact ttaatccagt cccttttcct gtcgtttacc caaggtgtca ccgaattttt
     8761 acccatctct tcttcaggtc atctaaattt atttcaacat ttgtttgggt tgacctcatc
     8821 actttctttt gctgtctttc tcaataccgc aacttttttt tccgtcatct ttttctttaa
     8881 aaaccagaca agatattttt ttgataattt aaagtacatt atcgtcggga ctatccccgc
     8941 catctttatc ggtctctttt ttaaagatca aatagaatca attttttctg gcccgacttt
     9001 tctgccttat ggatttattc ttacttcaat acttttattt tctactcgct ttgtcaggat
     9061 aaaagatgag aagctggatt ataaaattgc tattctaata ggtctgatgc aagccttagc
     9121 catacttccg gcagtttctc gttctggtgc cactattgcc accgggttgt tgcttggatt
     9181 aagtccaatt attgctttca acttttcatt ttgtcttttt atagttgctt ccctcggggc
     9241 catagttctt gattatcaat cattattttc cttatcactc tttagtccta tttcgctatt
     9301 atcttttatc accgggacac ttactggtat tattgctctc tactttctca agaaaattct
     9361 tcagaagaac aacttttggt attttggtat ctacactctc atcttagcct ttagccttct
     9421 cctgcttctc cgttaatcat atcttgagcc attttcctca agttatcttc agctgaccac
     9481 aatgctcttc ttctcttttt acctttttct tttttattct ctgcaggtgt gaaatagatt
     9541 atgtctttat caatcacata ttcaatatcc atagatcatt cattatacac taatcgcact
     9601 acttgcatat caaatacata catgttaaat tccttcaatc atgataagta acataaaaat
     9661 aggtgataaa tatccggagg ttgttaatac aattgtagaa ataccttcag ggactcacaa
     9721 caaatatgaa tatgacgaag aacttgacat tatcaagctt gatcgagttc ttcattctcc
     9781 ggtacactat ccctttgatt atggttttgt tccagagact aggtgtgacg atggtgacca
     9841 tcttgatatt atggtggtta ctaactctcc tacttttcca ggttgtttac tagaggctag
     9901 ggtacttggg gctttgttaa tgtctgatga acatggtggt gatgagaagt tgttagccgt
     9961 tcctgtccac aacccttatt ttaagcacat gaaatcgtta agagacgtct ctccccatct
    10021 tcttgaagag atagtccact tttgggagtc atacaaacat cttgagaaga aaaaacgtcc
    10081 tgttaagctt atgggttggg tttcccgtac tgaggcctat aagataatta aagcttctca
    10141 gaaaaaattc caagcagaga aaaaagagat aactacctaa gtagtggaaa ctttttctga
    10201 tataatattc tcataaagca ttattactga ggtcgcgact cagaagctga gagacaaata
    10261 ctctacggaa gttgaaccgt taaatccaac agtcgatatc ggtcaaaagc tagagtcggc
    10321 caaagtgtct aaaagaaaaa tgcttttaga aatcaaggtg gtatcacgag attgagaagt
    10381 cccgtccttg gaagttatta taattcccag agacgggatt ttttgtctca aaacaatatg
    10441 gcacaagaaa aagacagtgc agatatcact gagatagtga tagatcagtt ggtgagccaa
    10501 tttcatgact cagttaaagt tatggcccac aatccggaca aatactccga gatgttggca
    10561 ttgcttaaga gttacaatcc ccagttttca ccccgtcgat actaacgccc aatttcccga
    10621 gacaaaggtg cttgtcaatg tggtagccgc tatctacgcc ccactaattc atggttttag
    10681 tcagacaatt ctcaagaata ttcaaaccgg tattttgccg cctgaagtag ttgcgccacc
    10741 aagagacgcc attcctttgg ctaattctct cttagcacaa gcagaaataa gaggtataga
    10801 tttacatatt tttgagccac cggttaaccg aaatacagct ggaatagtta acaatcagaa
    10861 aagtagcgat gctaaggtaa gtccgtttct taatttgatg tttgaccaaa ccacatcggc
    10921 catgaacggc gccacttcaa tcgttgaagt cgaaactggt atttacggca ctacttccat
    10981 gattatggcg tctgctttca aaagcagagg gatagagtca tactatccat taaagtttta
    11041 tggtcttggc ccaaacttga gttttgttca tgcggtgtta tctaacggta aagagtgggt
    11101 ggcagaaaat gccgaatcct ttgggctagt gagtaaatcc caaattaccg gcttgatggt
    11161 tctacttgac accatggaag aactgggtat ggaaaaattt tatcaaagtg tcgagaactt
    11221 aatgattgat aaggacgggt tagttaaatc ggtaaagttg gccgtttcag aggaggagtt
    11281 agagattgcc attgctacca atcaagttat taaagataca gctccacttt acgaaaagat
    11341 accacttgag acattagttc agatgctagg agatgtgcca aaattagtaa aatgctctag
    11401 tgacggttat ccttttactt taaccgaggc aattccaccg atgaactcaa aagaagaaca
    11461 ctttaagtta gttcgatcct cgaagttatt tggttgtcca aaactaagaa tatgaaatta
    11521 gatttaaata aaaaaccatt agtcatcggc atgattcatt tacccgtcac acttagttac
    11581 gatggttggt cgggtctcaa tgcttttgtt aataaagcca taagggattt acattcttta
    11641 gaaaatggcg gtgttgatgc tgccttaata gaaaatgacg gtgatcagcc gtgccaggtt
    11701 aaaggtacgg ctgatgtcgt cgcttctatg acggtagtag ctcatgagct agccaagata
    11761 gccaaaatcc ctcttggaat agaggttctg ttaaatgatc caaaggcttc tttatcgata
    11821 gcaaaaacat gtgggctaag ttttattaga accgattact ttgtcgacaa aatgactaga
    11881 gagggctatg gagaatttga cgtcgaccca cttggattac tcactttcca acacaagatc
    11941 ggggcggaag atattaaaat acttgcggat gttcaggtca agtatgctac tatgatcgac
    12001 aaaactaaaa ctattgtcga gtcggcacaa gaagctatga ggtttggtgc tgatggcatt
    12061 ataattactg gcactaaaac tggagaaaaa ccagtcacca ttgatattaa ggctgccaag
    12121 gatgctgtag acagcagaat acctgtgctt gtaggtagtg gtctatcggt aaaaaatacc
    12181 caagaactgg ttaaatacgc cgacggattc attgttggct ctgccataaa aacgggtgat
    12241 tttgtcgatg ttctaaaggt aaaaagccta atcgcagaga tcaatgattg tcgatacatt
    12301 gaccacatca aaaaaacatg actttctcaa ttgtcgggaa tactggattc gaaccagcaa
    12361 cctcacgcac cccatgcgtg cgcgctagcc aattgcgcca atccccgggt tgattcacta
    12421 ttatattcgt tctaggttac ttttgctagt tttaatgttc ataatattgg ggtcagatga
    12481 gatatctttg ggttatattc ggttttgata ccttgacatg acctagtctg gattgttatt
    12541 actatctata ctattaattt aaaagttttt aaaaaaatgg ccaagaaatc attaaagaaa
    12601 gatatctcca acaaaaaccc gcttaccaag ggcactgcca atttagctga aaagaagctc
    12661 gaggctgtcc gtctcgccat ggaacaaata accaaatcct atggtgaagg ttctattatg
    12721 aaaatgggcg ataagcaggc agatatgaag atagaagttg ttcccactgg ttgtttaccc
    12781 ttagacattg ctctaggagt cggtggactt ccccgtggtc gtatcactga aatttttgga
    12841 ccggaagcct ctggtaagac aaccattt
//
LOCUS       DDWP01000100            6538 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_29587, whole genome shotgun sequence.
ACCESSION   DDWP01000100 DDWP01000000
VERSION     DDWP01000100.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_29587"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2099 a         1530 c          731 g         2178 t
ORIGIN      
        1 ctacaatgag ggcatatctc gtgatttgag taaccgatct tcgttcttat taaaaaatgt
       61 tcagtgtcca attgttggca atattagtat ggatatgact atgtttaaaa taccccgaac
      121 tattaacgct aaaatcggcg acactgtcac ccttatctct cctgatccaa gttcaatctg
      181 ttcagtttac aagatatcat ctattctaaa tacaattccc tacacagtcc tcacaggatt
      241 acacccatca actaaaagac gagttattta acctccaaaa cctaccattt ctgatagagt
      301 gattcagtta aaccgttctt tattcgatac tccaccaaag attttattgt taatattccc
      361 atcccaaatc tatccgatat ctctttaagc tcaggcatct ttgccattcg accgttatta
      421 ctcaatatct ccacgatcac tcccgaaggc tttagccctg ctaaactagc caaatctgtt
      481 gccgcttcag tatgaccttg tctttcaata actcccaagt cttttgccat taacggaaag
      541 cagtgccccg gataaacaaa atcgtcatgg atactcccgt cttcaattaa ctttttcatt
      601 gtcaaagacc gatcaaatgc agaaatgccc gtcgtcacac cactaaccgc ttcacaacta
      661 caataaaaag ctgtcgtcat aaaactactg ttcacttcac acatttcttt catctccagc
      721 ttctctagcc tctctctcgg caaagaaata catatcaaac ccctgccctc ttttgacata
      781 aaattaacaa tttctggagc taaattatct gttgctacaa aaaagtcacc ttcatcttca
      841 cgatcgtcat tatcgactat aataaaaatt ctcccctctc ttgtccagat tattgcttct
      901 tcaactgtta atatcataat ttttcaaaaa ataactgtgt atgacaatca aatccatatt
      961 caaaactaat attttctccc aaatctctta tcaatctttt accagcttct tctaactggt
     1021 cttcaagttc atcgtcagat aaccctgata aaattgatat tggtctaagc ctaaagaaat
     1081 ttatccattc gtcaacactg ctaaaatatt tttttacatt aatttccttt actccaacta
     1141 atttaaaacc accactttta agcatcttca cgatctctga agtatcaggt gttcgacgaa
     1201 gctcccaatc caatatcttt ggcatatatt tataattcca agtattttta agttggctat
     1261 gggaagttga tgcaactaac agcctaccac ccttctttaa gattcttctg agttcattaa
     1321 ctaataaaac tttatcttca acaatgtgca aaacatgata gagaatcacg ctatcaacct
     1381 ttccatcacc aaagagaatt ttatccccca aatcagcttc aaccaaactc acattcttga
     1441 gttttttatt tttaatatct aacgaacatt tatctaacat ccccttggcc ttatcaattc
     1501 caacgaaaga tacatccgga ttcctttcag ctaaaggtaa taaaaaccta cctgttcccg
     1561 aacctatatc aaccacaaga tttgccagct taatccataa atagctttca attgtctcta
     1621 ctatttctgt caatatcccg tcaggtaaac gcctcttttt cttataccca tcagccaact
     1681 tattaaggtc aactgactcc gtaattctaa gactcatatt tatcaataga tgccaaaatt
     1741 attaataata caagttcact aaaacttagt ttacctattt ccgccatttc tggataccaa
     1801 ctggtatccg ttaatccagg caaggtagtg atttcaagta gataaggaat atttgtcccc
     1861 tcttcaagtt ttaaatcaat tctcgaataa acattagcac ccaatatctt atgtgcatac
     1921 ctacaaacat cctttacctt attctcaaca tccggctcca tcttagccgg aataatcttt
     1981 tgatactttt tcggatcact tcttatcttc aaatcaaaaa aataaccttt atacaatact
     2041 tccaataccg gcaataccac agttttacca gaaatttcaa tcactccaac agacatctct
     2101 cttcccgtaa tatatttttc cgcaaaaacc ttgtctaatt ctttaactcc atttaatttg
     2161 ttttcaaacg tctctctatc atctattaaa aacacaccat taccagaacc ttcatcccat
     2221 atcttcatca tcaacggata tcccaacttc ttttcagatc tgtcaacaaa atctttagta
     2281 tcaatagact cactccagac aacatagtcc gcagtagaga caccttctga tctaagtatt
     2341 ctcttagtaa ttaatttatt cataccaaca ccactaccca gaatcccact acctgtaaat
     2401 ggtatacgat aaaaatccaa taaaccttgt attgttccat cttctccata accaccatgc
     2461 aaacccaaaa aaactaaacc agagttgtta gctttatcaa ctagccctcc catatcctct
     2521 tccggattaa ataaaattgt tttaattcca cagtccatca aacacttaac aattctttta
     2581 ccagtacgca aggacgcgtc tttctcacta gaccttccac catacaacac caaaatattt
     2641 ttacctccaa gaaatttatt tattttatca actgacctta ataaatccat ttaaattgag
     2701 atatattaac aaagctatat atcgatatga gacaggtctc tatatctaaa aaatagacag
     2761 acccaatcac accagcagac aattatatca cttttggcga aaagttacat aaacttatct
     2821 tttttcatct ctttttcaat cagtcctcgc aaaaaaactg atcttggaat cattcttttt
     2881 tgagccaccc aatctaagta attcaaaatc ttcggagata caaaaaaatt aaatctaacg
     2941 tccatcaaac ttctagtttc ttcaattgcc ttgtcaagtg ccactccgac ttttcccaac
     3001 tcatattcaa cccaaataag acgatcattc tttaggccca taaactgagc tggggccttt
     3061 cccttttcat acaaaacaat tgttggcttg tttttttcaa tcgaaatggt tatctcatga
     3121 ccaatatgca atgtcgaggg aaaagaagct tccataacgc acagatcaga tttatctatc
     3181 cacgcaatta tttgtttgta attatctatt ttttcttcat cagtcatgtc cagagtgctc
     3241 tttgatttat gatccacagt attctcaaat atcttacaac cacttttttt caatgccaca
     3301 aggatctggc tatagttctc atgcaattca cctttaaaaa ctctagatgc agaaaaatat
     3361 actttcatag atttcataat tcttaatatc tatattatac agctttattt caaaacaagc
     3421 gctattttta ctatttatat cttttattcc tcttcatatc cttttcaatc agctgcctta
     3481 tatgttcact tttgttagca attcccttat ttgataacac tctatcaagt tgagtcaata
     3541 agttcttagg aataaaaaag ttaaatctta catccatttc tttttcaatc ttcttcatcg
     3601 actcagttac tttttcttcc aaattatctt tcttgtattc acaaaccgac atatttctat
     3661 ccttgtttgc ctctaataaa gtcgtatgac cagtcggttc atgaactaac agaactttct
     3721 ttgataaatt cagcgcagaa acaacctcaa acgccaccga agcacttgaa tatgaaactt
     3781 cagccacaac aacatctgct tttttgatac tgtccaaaac attctcaaaa tttgtattta
     3841 attgtttact gctcaaattc tcaatatctt ttttatcttt attagttatc tggtcactaa
     3901 cttgataacc cagcttattc agtatcatca caattctaat gtaattatcc tgataaagtt
     3961 tcttgcctgt tatagaagct gtaaagtgga ttctcacttc ttttttacct ccttctcaat
     4021 caacatccta atatattcac ttttatttgt ccccgtgtcc ttccccaccc aatccaagta
     4081 cgccaccaaa cttcttggca aaaaaaagtt gaacctaaca ctaaccttac ctttaattcc
     4141 ttctaaagcc tttgttaaag attgagcgat atctgcatct ttgtacttta cccaaattat
     4201 catatcgttt ttataaccca acaaaaacat cggacttctc tcttccagat acattacaat
     4261 caccggtttt ccttttccta aagccaaact aatttcatgt ccaatactta aagtcgacgg
     4321 aaacgacgct tcaaaaactg caaaatcaca tttttctacc cactttctaa cattcttata
     4381 attatctaga atctcagact cactcatttc ctgaaattta gaaaacgggg tcacagtagt
     4441 atcctccata acctccaaac cattatcgac taaacactta acgatctttt tgtaccttgg
     4501 ttggaaatca ccctgagtat acaacgcccc cgaaaaatac actttcataa ttcattgtct
     4561 caaaaaacaa cccaacaatc caatttgaac tatctttatt tgttttgttt actcagcaca
     4621 taatccaaca tggttggagc ttccttttga gtatcaattc ctaagttact gtttagatat
     4681 cgtttgagtt ttaacttaat tttatctgtc tccaataact caccaaatgt ttctgcttca
     4741 ataaagacat cttcatctat tgaatcaaaa gaaactgtca cttccattcc gtcaaatttt
     4801 cctttgtaat cattccggat tttattgtat gtaattttgc tattttcttt aagtttttta
     4861 tattctaatt catttatctc gccctcatcc tctattcgac actttactcc tgataccatt
     4921 tcccacttct tggcagtttg gataaattta ccatcactag acctcaacct tacaaaattc
     4981 cagcccaact ctgtcttttc tatctctaaa aatgaatcga ccaattcagc gttgcttgaa
     5041 ttaataaaac ccaattcagc caaaatcaat aaacactttt taagttggtc atttgtaatt
     5101 ttgtatttga cctcaatttc attattttta ctttgcatag cgctttaaaa tattaacatc
     5161 atcaaaccat ataccaactt ctcgttttgc agcctccggg ctatctgaac aatggattag
     5221 attgtcacga caataattat tagaaccttc aaccggatct acacttacta catacgacaa
     5281 attatattct atagccaaat gtcgtatttg gcctggttta cattccagag ggtcagattt
     5341 acctactaaa cttctcatcc tttccacaaa cttgtcttgt ggtccttcgc cttcaacgat
     5401 aatcaaatca actcgttttt cggacatata actcgtaatc gggtggaaat ggggagtatc
     5461 tttaacattt ttataatgtt cttttattaa tctcggttca gccacttttc tttgtcgaat
     5521 cacaacagta gattcccgag taatcatgtc taatatttga gtttttaccg gttctactaa
     5581 tcccaacggc tttagcatca taaaagtaaa cgccttctcc ataatctcgt gttaattctt
     5641 aattatctat gattttacta ttatatatac ataagtcaat acatatagta atatacatac
     5701 taatatgtat acaaaagaag ttaaaactac ctcgttgctt ccaaacacct tcgaaaacat
     5761 tagacagaaa caatatctaa cactaaagca cgagttgaca atcggtgata aattaggtga
     5821 agttgaatgt aaacttgaac caaccaaacc accatacaac gccattagtt ttatggattc
     5881 attgttgaaa caatttcttc ctagagaagg aaattttcga ttatggctaa atagtacata
     5941 cgaaaccact gactatgtaa ctgtatttaa gtcaccaaga ggtggttttt cagcagttag
     6001 acaccccgat gacaatatta tcaggtttaa gaaaaaaagc tcccgtgagc cagtacatcc
     6061 ccaatacccc acgattagtt ttcgaacaga aaaaacaatt gatattgccg tacgaccaaa
     6121 tgctaccctt ctaaatctcg cagcaaattt atttgataca ccgctaagta aaataaaaga
     6181 aattggatca tattatcgaa caaaagttcg catccatgtc atttcaccaa aaaacagaat
     6241 ttatactatt aatatcgata acgttactga tgatgataat catcaattac aacaggtcga
     6301 aatgaaatat aaaggtacca ccgtaacaac cattcaagat caaaaacaac gcaaatccga
     6361 aataattaac gatattgtta atttgtacga aatgctaatt atgagaacac caaaggatga
     6421 ttttccctta caaccttctt atataactaa acgtgactgg ttaaaaacaa accatcttac
     6481 caatacttcc taaaccaccc cacactctcc tccaccgcct cggcccactt cgggatca
//
LOCUS       DDWP01000101            7975 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_42318, whole genome shotgun sequence.
ACCESSION   DDWP01000101 DDWP01000000
VERSION     DDWP01000101.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_42318"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
     assembly_gap    2580..2599
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2197 a         1714 c         1383 g         2661 t
ORIGIN      
        1 cttatgtttt ttaccattca aattttttgt ttgccgccat ttttctagtt cttttaggtt
       61 ttgttagtgg tgctcttaat ctattttatt gtcatattgt ctcagctacc aaaggtgacc
      121 atcagttagc tggttatgcc gaaatttatc tcggtccacg tttcaaacat ctggctcttt
      181 taaatctcat cctcctttcg tttggagcca tgactgctta tcaaaagctt ttttctagtt
      241 ttctctcagc actattccct gtcgttggca attattctcc atatgttttt attggcttac
      301 tcggtttggc aattatttct aaattcaaga tcaatcgttt cttttccatt ctcatcccac
      361 tttttatttt attaattcca attgttttgt ttttttattc tgccctattc ataaatcatg
      421 aatcatactt catatttcaa tctcctagtt tccttttcta cggtgcgacc atttttgctc
      481 tcagtggtct gaccattgtc cccgagatag atgaaacatt acgtcgcggt cgtcccaaaa
      541 aacatgagac tctggctctg gccagtttct tgggtgtcgt ccttgttgtt attacctatt
      601 ttcttttttc ttattcagtt gtctctttat caggctcttc ggtttcagtt aacaccgttt
      661 ctggtttact tgttaattct ccattttttg cgctggtaat ttctatcttt ggcatcatca
      721 ccactttccg agcttcttta aactttatcc atgttcttca tgagcttttc tttcgcgatc
      781 ttggcttatc cgagtcaaag tcagttgcct tatcttttgc ttttccactt ttaacacttg
      841 ttgccggggc gattcctctt ttgacgataa tttctctgac tgggaacatc actatttttg
      901 tttctgcact cttagtgatt cttattcgct ttgaattacc ccgttctttt tggaccgaat
      961 tcttcgccat tctagttttg gtttgcctct cccttgggct actgatctct ttcgtttaga
     1021 ttatcctttc ctcaccttgc acttctattt tgtctccgtc aacgagtatc tgttcgccgt
     1081 ccttgagtag ataaattttg ttaccagtgt attgttgttt tatattgtcg taaaactcgt
     1141 cgctataatg tggaaatatg tcaaaactta ctaatttcat cggttcaata ctccctgccc
     1201 cgatttcatt gtcggtaaaa ccatattctg aaatttctaa actttgtcct gctaccatcg
     1261 ccccagcact tgagccgatg aagattattc ttttgtcaag taatttttta atgtttttat
     1321 ctatgccaca ccttttaatc caatacatca aatatcccgg cattcctcca gcaaaccata
     1381 tgatgtcatt ttcttccagc actttgatta cagagtcatt acgataatct tccagaatta
     1441 ctagatttat ttctgctccc aaggcatgca ttgtttccca tgtaccacca tttttccacg
     1501 actcccaacc ttcttcaccg tttgcagcgg ttggaatata agctattttt ttattttcaa
     1561 aaccacccac aaatcttttt agactttcca tagaaatcgg gttactgact ctagaggcta
     1621 ggaatagttt catatgccaa ttttaacatt agttaccccc ccctcttgtc aaagaggggg
     1681 aaggcctgcc tgccggcagg cagggggaga ttgaaaacga gagcgcgtga tttaaatggt
     1741 aaaatgagct catgcaatcc caagtcgagg aaatcaaaaa aaagctcgac attgttgagg
     1801 taattggcaa atatctgccc ctcaaaaaac gtggtcgtca ctttatcgct tgctgtcctt
     1861 ttcatggcga gaaaactcct tcttttactg tctcttctga gttacaaatc tttaagtgtt
     1921 ttggttgcgg taaggcaggg gatgtcttta cttttgttca ggaattcgac aatattgatt
     1981 tcaaagatgc tctcgaagat ctggccaaga tggctggaat cgttctagtc aaaaacaccc
     2041 agctcaccga agacgaacgt cgtcgcaaaa ggctcgtaga tctcaactat caagtcgcca
     2101 aattctataa ttttatatta atcagtcacc cccttggtaa gccagctctt gattacaccc
     2161 ttggtcgagg gattactctt gaatcaatta aattgttcaa aattggctat tctcctcaga
     2221 accctgattt aatttttaat tatttaacta agctaggttt cactctcgat gagatggttg
     2281 ccagcggcac ttttggcaaa agcaactatc aaggccatcg tctatatgat cgttttttcg
     2341 accgtctaat tttccctctc gccgattttc gcgaccgcat tttaggtttc tctggtcgtt
     2401 tattgcctca ttcacaaaat cagaatactg ggaaatatat taactctccc gagaccgatc
     2461 tttatcacaa aagtcaaatg ctctatggtc taaacctgac caaagaatct atcaaaaaat
     2521 caaactcagt tattgttttt gaaggcgaga tagatttaat ttctccctat cagatcggan
     2581 nnnnnnnnnn nnnnnnnnnc ttttacatcg ttataccgat actctgattt taggtctcga
     2641 ttctgacttt gccggcaaca atgcagctcg taaaagcatt gagttagctg attctatggg
     2701 gtttgatatc catgttattt ctctgggaga taagtataaa gatcctgatg aagctgtccg
     2761 tgccgatcca ggatttttca aagaccaact taaccacaca cttcctgttt gggactttat
     2821 tatcaatagt gctatcaaaa gtcacgacac tagtaccatt cgtggcaaaa aagaaattct
     2881 ctctttagtt ttaccatttt tagtcaaaat ccaaaattca gttacccgct ccgattatta
     2941 caacaaacta gctgctcaaa ttggttccga cccttccgcc attgccgaag aggcaaacaa
     3001 aattagtact actaattttg tgccgtcagg tcggagtcca actcccgttg gacttaatga
     3061 cctgactagt aaaactgatc gtcttgaaga ataccttctc actctcgtta tctcttcaaa
     3121 aaagcctgat ctcttgtccc agaaggtttc tgctcaattt tctttttcta ctccccgttt
     3181 tcaaaatatc tttcttaatt taaccaaaat tgaagacgag ttcgacccca aaaaatttgc
     3241 cgatactcta tctcccgaat tatcaccgac tttcaataat ctatacttat ccggtactta
     3301 ccttaatatc gaatcccagg ccaggttaag agagatcaaa aaaactattt cccaacttca
     3361 atccattagt cttaaagacc ggatttctac cctaagtact aagatagctc aagctgagaa
     3421 ttctgacgat gatattctct taaaacagct agaagtagag tataatcaac tgttatccga
     3481 actttcaaaa gttcaatcaa caaaagccta gattaatcgg catttctcgt atacaatata
     3541 acccaatgcc aacttcctta aacttttcga aggtgaagca aaacttggtg gctaccgcta
     3601 agaagctgca ctacttgaca caagaagaga ttttggcgga ggtaccagag ccagagaaat
     3661 atattgatga gcttgattta gtttttgact atctttttga aaaaggaatt gatgtttttg
     3721 acaaagttga atcccaagcc ctgattgatg taaagaaaga aaaagccaat agtgccgaat
     3781 tattagcctt actttcccaa aagaacaccg ttgattcagt tagaatgtat ctcaaagaga
     3841 tcggcaagat cgatcttctc actttcgagc tggaagttga gtatgctaaa gctattgagc
     3901 tcggatcaga agaagcccgc aagaatttaa tcaatgccaa tttacgtcta gttgtctcta
     3961 ttgccaaaaa atatatcggt cgtggtctta ccttcctcga tttagttcaa gaaggtaatc
     4021 aaggtcttat gcgtgccgtt gaaaaattcg attggaaacg tggattcaag tttagcactt
     4081 atgctacctg gtggattcga caagccatta cccgtgctat tgccgaccaa gccaagacca
     4141 ttcgtattcc tgttcacatg gtcgagacca tcaacaaagt ctacaaggcc actcgcgctc
     4201 ttacccaacg tcttggtcat gagccagata ttgcccagat tgccgaagaa gtcggtatca
     4261 gtgttgagaa agtcgaggag atctatcgta tttctcaaga caccacttct ctggctactc
     4321 cagtcggcga cgatgaagat tcattcttag gtgattttat tgaagacact acccagctct
     4381 ctccttatga agaaaccagc atggagttac ttcgtgaatc catcgaagag gtcctcgagt
     4441 ctctcgatga acgtgagtca aaagtgttat ctttaagatt tggcttaatg ggggagactc
     4501 caaaaacact cgaagaagtc ggtaaaattt tcaatgtcac tcgtgaacgt attcgtcaga
     4561 tcgaagccaa agctcttcga aaactccgcc acccatctcg cagaaagaaa ctgcaggatt
     4621 atttagaata atctaaataa atcgagttaa gttcgtttct ataagccagt ttgtatccat
     4681 tgttatccag aaagctgtca atctctttac tcctgtcttc gataataata actttcgccc
     4741 tgtatttctt ccaatccagc cccttaagga tttcataatc ataaccctcc gcatcaatcg
     4801 atattaaatc aactttattc tttttaatat atttaccaaa aacatcttgc aatgttttta
     4861 tctctatttt ctgtacatca actagtttac agccactttt aatggcctta tcagcctctt
     4921 ttttggaaaa agtcgacttg acgtgtgggt ctagacgata aaagtttttt gtacatcttt
     4981 tatcacccag tcctatatta aggcaggtat cttttggacg acactttttt atttcttcat
     5041 atagatcttt tattggttcg attaccactc ctcttgctcc gtttatataa aacaaatagg
     5101 tgttattaaa cttgatcgcc tcgtttgctc cgatatcaac atagccttta atcttgtctt
     5161 tacctatatt gttagctatc cacctatcct caccatattg agaataagag attttgagcg
     5221 ttctcaatat tattcttttt atttcaatta gtgagaatat gacaaaatac tttggtccat
     5281 aaagtctgta tttaaatacc agatcttgga caaaatttct tttcattagt taccaaacat
     5341 aatctctctt atttctccgt ttgctagttt ctcttgtttt ccctcggtca aaaacaatgc
     5401 catgcttatc accatttgat gactgatcac cactacaata tcttcttcac tgtggtcata
     5461 ctttagatca tcgaggaaaa gttctactct agcttttaat ttttcataat tttccgactt
     5521 catttttggt cctaccaatc ggctaaaatg tattggaaaa ctatcatctc gaattcgcag
     5581 tatttcatcc atggtttgtt cacatcgagg ggtttgtgag caatatattg cactaatctt
     5641 ttttggtgcc aattccgccc ctacttctat tgcttgtttt acacctactg ccgttagatt
     5701 attttcctct cctaatacct cataacctct gtttctcgca ctttcaccat gtcttattaa
     5761 tattagtttc atgcgtgtat tttaccaatt attacagcaa taagtttggt tttaggcgga
     5821 gtccttctct acagacatat gttgtctttc cctcacaagt tagcctgtcc aaggtaacta
     5881 tttcatgatt agaatgtttt ctagcctcga ccattactac ctgagctgta actggctcat
     5941 gtgtaatttc acctattatg tttacgcagg tttcacatac tgcgatcgac cgtgtctttg
     6001 cacctattgt tctcattgtt tataaaaatc tctattattt agatatccag ggatattaac
     6061 aaagacatgc cgttttgaga agctagtcaa ttcaactctc tatcagtccg tttgctttat
     6121 aatctcttta tggccaatct cgattctaac ttttccaaag agatattgtc acccactccg
     6181 atcaccagat taaatgttgc tattcacccg ctctggagaa tgaaaccctt gaatcaaggt
     6241 caaatcttta aggatcgttt tgttgctgct cgacaacatc tcgctgaggc tgttgtcgcc
     6301 aatatggcgc catcttctcc cggtgaggtt acctgtttta tcccccacat tgttaattct
     6361 cgaaagtcag atgagttagt tcatcaaaaa caaaaaattg ctactaatca gcaatatctt
     6421 caatggacga cagtttttca agatctggta accaataccg atccttcatt ccgacgacag
     6481 ctccttattg ctcccaatct tgttgaaata cctggacatt tgggacaagg cgaggtagac
     6541 ccagatattt ttgttcaagc gctcgccaag aagggttttg ttatcactcc cgacacagaa
     6601 gttgttgtgg gtggtgagat gctttacccg tgtgttaccg aaacagttat gcgtcttctc
     6661 gaaatagggg agattcatca tttgagagta gacaaaggta ttacactgac ctctgactac
     6721 cttatttctg atacagatac ctcagttggt cgagagtatg atcagttcgt ccagcgaatg
     6781 tctgcgttgg gttacgacgt tattcaagat catcgtattg tctcgataca caaaagagaa
     6841 tctaaattgt aggctaagcc ttgtacactg cgtttcgtta ttctataatt tatcatgggc
     6901 tatgctggta agctagagaa aaaagaaatt gccaaatcac ttcgtaagaa aggatattcc
     6961 tacaatcaga tcaaagcagt cactggttta tcaaaagata ccatctctcg aagttgtcgc
     7021 gatatcattc tcacaccaag tcagataaaa ctattggatt taagtcgtag aagaggacag
     7081 actttggcct caattaaggg agccaagtca aatcagttga aaagaattca aaacgaaaaa
     7141 gcattgcttg aagagggtgt aaatcaagtg ggtgctttaa cagtcagaga taagtttatt
     7201 gcgggtatca ctttttatca gggtgaaggc tcaaagacta gtcatgccgt tgagtttact
     7261 aatgctgatc cagaaacaat aaagttcatg gttgattggt ttgagcagtt ttgtgagatc
     7321 aagcgagaag atttgaagtg tagcctttgg ctacatgata acttagatga agacattgcc
     7381 aaagaatatt ggtcaaagtt attgaaaata aactgtttac agtttggtaa aacatatttt
     7441 gccaaaaata agccaaatag tccaaaaatc agaaaaaaca tacatcaata cggtattatt
     7501 aaaattcgtt attatagctc gacaaaacta cgccttatct taggttggat cagaggtgtt
     7561 ttaaccaact agattgaatg ttataattct caagctcttt taaaaaatag atcttccccc
     7621 ttagctcagc ggtagagcgc tcgcctgtta gttaaatagc agcttattcg agtaatcgag
     7681 tttgaaaaat cgggagaatt gtcctgaaaa ggagtccgta tctcgcaaga gcatcggacc
     7741 gtgaaaccta atcctaagag aaatcaaagg aatggcaacc cgcagccgag ctccgacttc
     7801 gtcggagaag gttcagagac taggtgagta cacctttaag cacccgagtc cctatttata
     7861 gggatatgat atagtccgag ccttatagaa atataaggaa ttcttgtaag cgattggtcc
     7921 gaagttcgaa tcttcgaggg ggagctagca aagaaagccc caaacatggg gcttt
//
LOCUS       DDWP01000102           11955 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_24987, whole genome shotgun sequence.
ACCESSION   DDWP01000102 DDWP01000000
VERSION     DDWP01000102.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 11955)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11955)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11955
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_24987"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         3311 a         1839 c         2884 g         3921 t
ORIGIN      
        1 aggttgaaag catcttgtcg attgggaata ctgtggtaga taaacttctt attttctggt
       61 gagactccag caataatttg tgaattgatg ttctcgactt tttcattacg agtgtcgtcg
      121 gcagtaatta tggctatgtc ggcgatttga gagacaactt gacccataat ggggcgctta
      181 ctagggtctc ggccaccagt ggctccaaaa atgactatta atttgccagt agtaactttt
      241 ctaagcgatg caaggacact agagagagca tttggggtgt gagcgaagtc tactaagcaa
      301 tgtattccca gattgttgct gacgatctcc tgacgacctt tgacttcggg aaaatgttta
      361 atggcgttaa tgatgactga ggagggtact ttaaggctat ctagaactgc atgtgccgct
      421 aagatattgt atgtttggtg ttgaaatgga ctattggtct caaagaggta tccgcctact
      481 ttgaacttga ggtgtttggg aaggagttta atgtcggtgg ccaaataatc ggctggttgg
      541 gtagtgctgt aggtttttac agcagtgtta atgtggcgca atatgtgttt ataggattga
      601 tcgtctttat taaggattgc taggctggct gttttgaata tctttgactt agtgtggatg
      661 tagcttgaca tggtggtaaa gtcatccaaa tgttcatgtg aggtgttggt gataacggca
      721 actttaaagt ggcaacctag gaaacggaat tggtctaagg cgtgggcggt aacttcgata
      781 accacatggg ttatcccgtc attgaccatt agttttagaa ttctttgaac aattgaaggg
      841 tctggtgaag tggtgtgtag tcctagagta atttctttat tgtctatttt tgcccctacg
      901 gtggagaaca tccctgtcct aactcctgat tgttgaagac aatggtggat taagttggta
      961 gtagtggtct tgccatctgt cccagtaaca ccaataatag ttagtttttt tgaaggttga
     1021 ccgtagtata aatggtagaa aagtcctttg gggtagtgcc aaagataatt ttttactttc
     1081 tgaaaaaaaa ttcgaaatgt ttttttggtc ataaaatgca attgaaatta tagcagagag
     1141 gagattttac tggctaaatc gtaccatatt ggagcagctg tgctagatcc ccaaggtgat
     1201 gtcttgggat tgttgatagt gacaatcata gtaaattttg ggttctgaca agggctgaaa
     1261 cctatgtaag aagcaatggt ggaagaatca gaatagcccc ctttgatggc gacttgggaa
     1321 gtaccgcttt tagcgcaagc ttctaagttg tctggcttaa gactggcaac aactccgttt
     1381 tcgactgaat atttgagtat ggctttaatt tgagagacgg tactttcttc ataaactttt
     1441 aaaggggtgg ggattttctg aggagttttg taatcggagt cgataaagta ttcaactatt
     1501 cttggtttta ccaaatgccc gttgttggcg agagtgttaa aagcggtgat catttggatc
     1561 tgggtaatgg caaatccttg cccaaaagag gcggtggcca agtctatttc tggccagtat
     1621 gatttaataa tgggtttgga ttcttggggc agatcaatac cggttttcct gtctaagttg
     1681 agaagctgaa aataattaag aaatttcttg agcccaagtt tttcaataat ataactcatg
     1741 ccgatattat cggagttttt gatgatatct ttaatgtctg aattggggtg aaagctgttg
     1801 tcccagttgc taacggcata ctgacctatt tgtttgggtg aaccgcatcc tgaacagaca
     1861 aagtcactag agattgattt tgaatcgaga gccatagcaa cgactagagg tttgaaaata
     1921 gatcctggtt caaaaagatt ggaaactggt gggtttttga tatgagcact agaggtggct
     1981 gtgtcttttg aaaagatatt gcttagagcg ataatagatc cagattgagg tgacatgatg
     2041 ataattgagc cagaatctgc agaatagtct tctattccct gagtaagttt ttgttcaatg
     2101 agtgattgga tttgacgatt gattgatgtc tgaatacttc gtccattaac aggttctatg
     2161 taccagccag atttggaaag tatagttttt ccggtagcat cgacagtttg ccagacaaaa
     2221 cctgacttgc cttgtagttg ccggctataa tagttgtcta gaccatcgac gactgatttg
     2281 gctagatttg cttcaggata aaatctgttt tggagaggag aaaatttaaa accagggtcg
     2341 gaaagctgtt ttttctcggt atggctgtaa agacgatcgt tgacggtaat ccatttgctg
     2401 ttctgattgg taataaaact atctatctga ggggtatcca tttggatggt cttggccaaa
     2461 tcagtgaggg gtagttttag atctggtttg tagattgaaa gttgatattt tgtctcattt
     2521 aacatcaatg gatagccgga gctgtcatag attttgccgg catgtggttc gatgactgaa
     2581 ggtttataat tttgattgat gtttttttgg ataaagctcg gatattttac aacttgccaa
     2641 tagaacaatc gagcgatgac aactaggtaa agaaaagcga tagtggtgta gacagttttt
     2701 agcctggtct tattcatatt tatcgaaggt caagataaag ttttgaaggt ggcttggaat
     2761 ttgggagaga attgagaacg gccatttcat tttcaagaac ttgattctct attcttaatt
     2821 cggtaatttg tgattgggat aaattgacct ggctgttctc gtcgacaata gtaatcgaaa
     2881 aataaataga gaagacaatt cgagttagaa gtgtaaagag gctaaccgca aataaaattt
     2941 ttaagtgcat taacttattc tatatgatcg gaggactgca gagcgagata aagggttatt
     3001 tcttatttct agagtggtgg gtaaaatggc ttttggtgca ttagggaagt ttgcttttat
     3061 aaattgttta acgatacgat cttcgccaga atgaaaagag atgatggcaa tggtggttcc
     3121 agataatttt ggattgaggg ttaattttaa agtatttttt agattttcaa tttcgttgtt
     3181 gacgactatt cttaaagcca tgaatatttt tgtgctaggg tcgatagtag tgcgaatatg
     3241 ttttgattga taatagtcgc gaatgatatt ggccaatctc tcaccatctt ttataggttt
     3301 tttctgacgc tctttgatga ttctgataga gagaggtttg ctatatgttt cttgggcata
     3361 gagagtaaaa atttcacaca attcttcgta gtggtaagta ttgataatct cctcggcagt
     3421 tagttcttgg gtatcagggt caagtcgcat atcgagtgac tgattatcgt tgaatgaaaa
     3481 tcccctgttt tgggacaaaa gttggtttga gcttagacct aggtcaaaga gtatcccctt
     3541 gattggcttg tcgatttggc taataacctc gttgagttgg ttgaagtttt tattgatagg
     3601 gataaagaga ggtgagttta tcctatctgt ggctatctgg aggttagtgg gatcttggtc
     3661 taagccatag acgacagaac cgtgttgggt gagttctagg gaatgaccac cattgccaac
     3721 agtacaatca ataaagatat cgttggcctg aggatcaaag gtgtctatca cctcttgtag
     3781 taagacagga gtgtggtatt tcattcttga gcagaatact caccaactag ggaattgctg
     3841 gtgttgctat agactgaata tccaccacga taattttcca tgaggatgaa gaagtctttg
     3901 gcagctagtt tagcttttgg tgtgagtgag aagttggcga cagggagtct gatttggccg
     3961 tctttggtta tttgagcacc agttttaaga gtaaatggtg tctcaaaact gaatatttga
     4021 ccagcattgg tgatgaagtt tagtgtgagt gttccaccgg tgacgccttc gtcgtatttg
     4081 tacttacagc cattggtaca actttcggta ccaagaagta tttttctttc gatgatactg
     4141 gtaatttctt tgatggtgtc gccgacacct ttttcaattt ccgatttacc atcgacagca
     4201 gtatagatca cctcgtactc tatttggctg acggcgctcg gcatttggga tatcttaaga
     4261 taaatcatcc ggccatcgtc acgaggagtt aggctaacta gcggtctgtc ggctggggcc
     4321 atttcgatta gttttggagc aggcgtcgga gtgggagctt cagttttctt ttgaggccca
     4381 caggcagaaa gtaaaaagac gagaggtagg ataaataata tttttttcat agtttaattt
     4441 aacttcttat atagccaact gtcaacttct tccattttga tacgagtttg ttccatactg
     4501 tcacgatcac gaatggagac agtttgatct tctaatgttt gataatcaac aacaagacac
     4561 cagggtgtac cgatttcgtc ttggcgacgg tattttttac caatactgct actatcgtcg
     4621 tagtcgattg agtatttctg acttagagtg gagtaaattt cttcggcaat attaataagt
     4681 tctggcttat tggcagctag aggtaatata gccatcttgt aggcagatat ttgtggattt
     4741 attttcaacc agatgcggtc attttcctct tggtaggcat cgaggagtag gaagaagaac
     4801 gatcggtcaa caccacctga agtttcagtg atccagggta gatatttttc gttggtgatg
     4861 gggtcacgat aggaaagatc ttggccgcta aacttggaat gttgagtgag atcgtaatct
     4921 cctcgccagt ggataccttc catttctgtc catccccaag gtgaattata ttcgatgtca
     4981 aaggcttttt gagcatagaa ggctagctca tcggaagcgt gttggcggaa acggagagag
     5041 tcagtagagt taaaaagttt ttgataccag ctcatacgag tttctttcca atactcgtac
     5101 catttatcca tttgcgaggg gtggacaaaa tattggacat cccattgttc aaattcgcgt
     5161 gtacggtaga ggaagttttt gggagtgatt tcgtttctaa aagctttacc gatttgagcg
     5221 ataccaaaag gaattttgac acgagaactg ttgacgacgt tttgaaaatc tagataaatt
     5281 gtctggcagg cctcccctct taggtaagca ggcatttttt caccttcgat aactccgatc
     5341 tcggtgtgaa atagaatgtt aaattttttt ggtgcagtaa attcaccatt acattcggga
     5401 cattttgtag cgttatcaat taggtcgagg cggaaacgtt tgtggcagga tttacattcg
     5461 accatttcat caccaaagtt tttgacatga cctgaagctt cccagactcg agggtgagtg
     5521 ataatagatc catcgatacc aacgacatcg cgtcggtcga ggacattgtt tttccaccat
     5581 aaatttttcc agttgtattt cattatcgat ccgataggac cgtagtcgta gaaaccttgg
     5641 actccaccat atatttcaga attttgaaag acaataccgc ggcgtttaca caaagaaaca
     5701 actttagcga caagatcgtt ttggtttttt tcggacatga ttccttaaaa tttataattt
     5761 taaggggtcc ttttggttaa ctcaggacgg ttccaccctt tatttcactt aatttggttg
     5821 agcaattata gggagccaat cccctattga tgtctatatt atatcgtaat atcttgatga
     5881 agttataaag ataagcgagg atcattaagt ggcctgatat aatttgatat gaagaacagg
     5941 agattatgga aagaaacggc agatatagat ttaaaactgc caacggtaag gatttactat
     6001 gtaatatttg taatattgat acctgtatct ttgttgataa ttaacccatt ttctatgatg
     6061 gcagactggc agtcgggaag gtggcaggat atttgggcat tgtggttgag ccataaagta
     6121 cattggattt tttggccaac ttatatttgg tcagttgttg ctctaatact tttaataaaa
     6181 gattaccgga aaaatagtaa taaattgttg gtaaggtggg gtgtatatgt gggaactttt
     6241 tggtgctgga tgtatttggt atacgaatta atacttttta tgccgggaat attgataggt
     6301 accattagtt tagaaccaat ggtcttagta cttggaagta tattaattgg agctggttgg
     6361 tattggagaa aaaaaattaa atataaacat ttgttaggga tatcgttggt tgtgatgttg
     6421 gtgtttgtag cgcttttttc aaatccaacc gtaaataact acttctactt taataaaggg
     6481 atatggtttc tcatattagt tttggttccg ccatcggcaa catggttggc gacgattttg
     6541 agtatgaaga tttacagaac gcctggcaat agatatggtt ggaggaattt attgattttt
     6601 tggggttatg ctagcctgag tttggctgaa tctattttta gattaattag tgaatacaac
     6661 aaattaccca aaactccacc aagttgcttt atcgtgacag cagcgacaac tgggcatact
     6721 attgtggtgg gcaaagaaaa aacacagttg cgtaactttg aaaattttga aaaattgttg
     6781 atggtgaaga agccatatct gcattatcgt ctacgtcgaa tttataacta tgctggcccg
     6841 gtggtggcaa atataattaa taagaataga tttttagcag atttgactta tattttactt
     6901 aagcctgttg agtggatttt ggggttaaga aaacatctta ccgctttgga ggggtttttc
     6961 aagaattgat tctaggtgtt ggcggattag tttttggcaa agaacatact ctttgagctt
     7021 gaaagtgtga gttatttctt caggtagacc aaagccaaga acagaaatca gctttatctc
     7081 ttgatctatg aattgagcta attggttgtc ggcaattgat tggataattt tttcggatat
     7141 ttcgtagact tgggggatat attggtcttg agcaataaaa ttattgatta tctctagaaa
     7201 ataaaaaagg agattgattt gagtaagttg ttttggttgg agtaagaatt gagaaatact
     7261 ggtagcctca gaaagccaag tatagccatt tttggtgtag agatgtgcct taattgtgtt
     7321 acctagctga aggctaccaa gtcggctact ttttatttgt ttggcacctt tagcagtggc
     7381 gactatcttg cccaggtttg gagtaagtag ggttactaaa atatccgact cacgaataga
     7441 ttgacttttg atgactatgg caagcgtatt ttggtgcttt tccatctaga gtgatatttt
     7501 gaccactttg tcagctaccc aactgaactt ctcaaactct ttgtcgttga ctaaaagttc
     7561 atattcgaca gccttagttc ctggctgttt ttgaagaagc agagtatagt ctttactggc
     7621 agcgacgcta ctagtgtatt ggatagaagt cttgctacta gactgagcat ataaaggata
     7681 tttattgccg tagaatcctt caaagacttg tttgcctagt tcttcataaa gcactggctc
     7741 gacttctgat ccggtcattt ttatgagttg gcttccagga gaaacataga agcggaacat
     7801 gtcgcgatat ttaggagcat taagacacaa gtcccctttt tctagattac agtttgagcc
     7861 tgggctgggg ttggtatagg tgactgagac tttgtgctcg actctacctt ggttggtagt
     7921 gatctcatgt tttatttttt gagtaataaa gatattactt tttgctgaag caaagttagc
     7981 atcatttaag tggaagtaat cagtcttact gtcagttggg gtaatattac cagcaatgtt
     8041 gactaattgg gctgatgctt gaatttcttc atcaggaaaa tagaacataa tatgtttctg
     8101 atgaatgttc ttgaagaagg cttcgcctaa tagaggaatt ttgtctttgg ctgaacccat
     8161 ggcattagcc aaaagtgctg acataactgg gccaaggaaa cctttacgat ctttctcgat
     8221 gtagtcacgt ggtcgaccgg agatccattc aagatcataa ataattttgg gacaaccgtc
     8281 acaacgtttg tctggttcgg tggtaaaggt gccaactcga gtgtcgagct tacctaatac
     8341 agcgacaaga tcaacaagaa cttgggtgtc gagagcaacg actgcatcaa attgttcttt
     8401 tttgctgata accttgtaat aagtttctag gaatattttg gcaccagtgg gaaagtcagg
     8461 agacaaattg ctatcacgga gatttaagta agggacgtta atgtgatagg ccttaatttg
     8521 tcgtggagct gggatgctac ttaataattt gtcatctaga tcgtagatat tgctagctgg
     8581 accaggaata actttaccgt tatcaacttt gagggtagca taggcagtcc agaagccacc
     8641 actaggtcgt agttcaccat cgttttggaa aataactaga taatttctgg gcttatcttt
     8701 gccaagtagc caagaagttt tggagatgat tggcttgccg ttactgatta agtttttaac
     8761 ttcggataaa gtttctttgc cttggttgaa gatggagtga acattaatat ttttgtatgt
     8821 ttctggatat cgatttatat ctatttcttc gataatgttg tcaatctcga cgattttctt
     8881 ttcgattgta tctaagtggg gtaacagccc ttcaattgat tgagtcagga aggctattct
     8941 gtcttcagta gtttttgaac tatcaactcc agagccttta agtccaagaa aatcttgata
     9001 aggttcgatg gcggtgatga ctatttcagc agtatctaag ccatgctcac cgatagtaaa
     9061 gactctttgg ctatcgagat aatagtcttt agcgtaagga agtgcttgaa gataggaaaa
     9121 tttttgactt gaactacgga ggtcttttaa ttttgctctt aggttagtaa gctgcgattt
     9181 ggtggcgaca agatctttgt cgttaacagc ggctttaagt tggtcaatat ctgtttggag
     9241 ggttaaagct tgggcataaa gtttttggcc agggacgtag acagtaaaaa atagagttgc
     9301 ccctataacg gaaatgacta tgaatagtat ggacagaaca gttaaagtta tagaaagaat
     9361 ttttttcttc ttactaattg gaggtttgat taaaataggt ggggtggaat taataggagt
     9421 aatgttagtt tctgtcatat ataaaattgt atcggaaaaa gccgacgatt accagatcga
     9481 aagcataaca aaaggtgttt tgagaataat tagaagttct tgtcgaagtg aacggttttt
     9541 gatgtaatcg gcgtcaagtt tggctctttg ttcaaaattc atctcgtttc tgcccgatgt
     9601 ttgccagagt ccggtaatgc ctggtttggc agtacgaatt atatcgatat atttttttga
     9661 gctaggaaat tttctttcgt actcttcaag ctcttctttg cgataagctc gggggccaac
     9721 taaactcatc tcccctttta gaacattaat aaactgagcc atttcgtcaa tagaagtttt
     9781 tctgataaaa cggccgatag gggtaattcg aggatcttgg tccataggaa gtttccaacc
     9841 atcttcaatg tattttttcc ataactcggg atagttgtca cgaaggaaag catccatgtc
     9901 gtttttgtat ttgttgccgt tgtacataga acgaaattta tacatgacaa attctccact
     9961 gttaccgact cttctctgtt gaagtaaagc gggcccctca gaagtgagtt tgattagaat
    10021 agcaattaaa agtaggaatg gtgagagaaa gatgaggaca aataaagcac tacaaatgtc
    10081 gatggttctt ttgctgacat taaaaataaa tactcgagtg cggtagaacg gtttttgggg
    10141 gttgttggca gacattttag tgaagaaaat aattttttaa cttgggacta gaaacgattt
    10201 gagtaattag atctctgaca aaaaaactgt cattgagatt gcaaacgaga agcgcgtcgg
    10261 gagtatcgat aatggctaaa ttgtgggcac cgacgaggcc gacaattttt tttggttgag
    10321 tatagacaag gcaatctttt gatttgatgg aggtgaggct ggtatttttg ccgatagtgg
    10381 ctagaccgtc ttggtttttc ttgagttgtt cccagatagc tttccattcg ccaatgtcac
    10441 tccagatgaa ttttgcggga agactggtaa gagagtttga tttttccgag atagcgacgt
    10501 caaacgataa gttttctgat tgcttaaaga tagtgttaac tgattttggg ttcttgataa
    10561 gttgttgata gagagaatag tatttgggtt ggtgagactg gagctctttt tctagagtgt
    10621 tgagggaaaa agtatatatg ccagagttcc agaaggcgcc ttttttaata tacagttggg
    10681 catcgttaat tgatggtttt tcgacaaatc ttgagtcggg tagtatgtaa ccaaatgatg
    10741 gattggggta ggttggctta atgccgatgg tgacgatttt ctgaccagac agagcgatct
    10801 tggcggcaga gttgatagct tgggaaaata tggcttcttt actaattagg tggtcagcgg
    10861 gagcactggt aataatggct tgtggattaa gtcggtgaat aagagcagag ccatagataa
    10921 tagccatggc ggtatttttc ttttctggct cgacaattaa gttttggcgg ggaagatttt
    10981 ttagttgttt tagaatgatc tctttgtatc gggcgttgga gacaatgaag atatggctag
    11041 tagggacaat tttttgatat cgggaaaagg tttcttgaag aagagttttt tcactaaaga
    11101 gactgaggaa ttgtttggga tttgaagcac gggacatcgg ccagaggcgc ggtccggtac
    11161 caccacataa aattaggaca tagtggttag atgttgttgc cataaatggt cgactgattg
    11221 tttaaagttt aacataaaac tagagtgtga gaacttaagg gcgtgttggc ggattgatga
    11281 ggaattaaat tttgctttgg taaaagtatc gatacttgcc tttagagatt cgatattttg
    11341 ttcgtcaaaa agaatgcctg tcttgccatt aataatggtc tctgtgttgc ctcccctatt
    11401 gtaggcaatg actggtttgc cacaagattg agcttcgata gcagctaggc caaagtcctc
    11461 taattgaggg cagatgagag cttggcagtg ttgataaaga gaaagaagtt tggattcgct
    11521 aacttgtcct aataatttaa tattgtttga ctcactggcc agattgacaa gataatcgtg
    11581 ttgacggcca tcaccggcaa tgatgagttg gtaaggtaga gatttaaaag cattgacgac
    11641 gaggtcggtt tttttgtggg cgacttgcct gccgacgaca agaaaataat tgttttgtgg
    11701 atgtttagtt ggtttgaaaa agttggtatc gataccaggg ttaataactg taggtgtgcg
    11761 atggtaggcg tttttgagac gagctttgac agtttgagaa ttacagagaa tagcatcagg
    11821 cctgtgtccg tagagttgat ctattttttg atatggcctg aggatggggt aactatagag
    11881 gtgacgattg atgttgtgaa agtaagttag aaagagtgtg tgaggtggag tgagcaagca
    11941 atgcccaagt acaga
//
LOCUS       DDWP01000103            4680 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_39793, whole genome shotgun sequence.
ACCESSION   DDWP01000103 DDWP01000000
VERSION     DDWP01000103.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_39793"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         1546 a         1028 c          868 g         1238 t
ORIGIN      
        1 aggttcgagt cctcttagac tcacagaact attactaaaa aatttatgga aacacaattt
       61 agagaagttg taagtagatc tgagaagtgc gaagctacag agctttcaca tatcttaaac
      121 actcaattct ctggacgtaa agttttgata gaatcgtttt cactcgacaa tagtggacaa
      181 ctaatctctg atccagaaga ttcaggagaa gttataatga ccagagctac ccctacagat
      241 actggtgttg aatccggata cttcgttgta gaatctttac agtcactact tgaaagtgga
      301 gagccagccc aagaatattc actgctcttt ggtgaaacat ccgtattgca cgatattaca
      361 gaagaagaca cacccagaaa cgtcattaca gttatcgaca atccagacca aagtcatcca
      421 tcgacaagaa aagttgtcag gctttcgact acatcgccca ttcgtgggta atttttgttt
      481 cccccgcttg ggctaccggt ttcacttttc tcaattagta ttttttaact tataattcca
      541 ctatggaaaa tcaaaattta cacgacattc gtcattcagc cgaacacatt ttatctcaag
      601 ccatgattcg tctttacgga cctgacgctt tttatatggc catgggtcca gctaccgatg
      661 atggctttta catggacttc gaacctcgtg gtgattttaa agcttcagaa gaagatttct
      721 ccaaaatcga agccgaaatg caaaaaattg tcaaagagaa tctacctata gttaggaaag
      781 aaataactct tgaacaagcc aaagaaatgt ttgccgacaa tccctacaaa atggaatggc
      841 ttgaaaccat taccaatcgt gacgaagtca ttaccatcta caccaccggt gatgaattcg
      901 tcgacctctg cgccggccct catgttcgct atacctccaa agtcaaagcc ttcaaattac
      961 tttctattgc tggcgcctac tggcacggtg atgagaaaaa caagatgctt acccgcatct
     1021 acggcaccgc ttttgaaaat aaagaggctc ttaacgaaca tcttgcctta ctcgaagaga
     1081 ccaaacgacg agatcaccgc gttatcggca aggatcaaaa gctattcact ctctcaaatc
     1141 taatcggcgc cggcctacca ctactccagc caaaaggtat ggtaattcgc caatgtatcg
     1201 aagattatct gtgggagatt cacaaaaaca aaggatatca acgtgtttgg actccccaca
     1261 ttaccaaaga agctctgtat atcaaatccg gacatgccga aaagtttggc gacaacatct
     1321 ttcgagttca aggtaaagaa gaaaaattct ttatgaaacc catgaactgt cctcaccaca
     1381 tgcagatatt tagtgataac caattcagtt atcgtgacat gcccgttcgc tactttgaac
     1441 cagcaactgt ttaccgtgac gaaaaaacag gagagttgtc gggcttaact cgtgtccgtt
     1501 caattaccca agatgatggt catctatttt gccgtgtttc tcagcttgaa gatgaaatct
     1561 caactattgt atctattgtc aaagaattct ttaccaccat gggtatgctt gatggctatt
     1621 gggtcagact ttctgtccgt ggccccactg gcaaatatct tggtactgat gaagtttggg
     1681 aaaaagccga aaccgcacta aaaaacgttt ctgaaaaaga gaaacttcct tacaagatag
     1741 gtgtggatga agccgctttt tatggcccta aattagattt tatgtttaaa gatgcaatta
     1801 aacgagaatg gcaattatct accatccaat gcgactttaa tttacccgaa agattcgatc
     1861 tttcttttat taatgaaaaa ggagaaaaag aacgacctgt ggtcatccat cgggctattt
     1921 ctggctctct tgagcgtttc atgggtgtca tgatcgaaca ctttgctggt gccttcccag
     1981 tttggctgtc acctgtccag gccaaaatta tccccattac cgatgctcaa catgaatttg
     2041 cctacaaagt catggatatg ctcaaagagg aggggattcg agttgaaatc gacgaccgat
     2101 ccgaaaaaat gcaagccaaa attcgcgatg ctcaaatgca gaaagttcct tacatgctgg
     2161 taattggcgg ccgtgaagta gaatccagtt cagttgctgt ccgccaacgc gacgagcaag
     2221 atctcggcgc tatgaccatt aatgatttta tgaccaaaat taaggaacaa attaacaata
     2281 aatccttaaa tttgataaaa taactgaata ttgtagagac gccccgtcgg ggcgtctcta
     2341 ctaaaaaaac aaaaatggcc acatatagaa atcaaaatca aaacaaaaag ttttacaaaa
     2401 taaacaatta catttcagcg accgaagttc gtctcatcga cgaagaagcc aaacagatcg
     2461 gcgttctcag cattcaagaa gcccgtcaaa tggctcaaga gactggtctt gacttggttg
     2521 agattgctcc tcttgccaaa cctccggtcg tcaagctcat tgaattcacc aaattcaaat
     2581 accaagaagc caagaaacta aaatccgaaa agaaaggatc taagggtgga gagaccaaag
     2641 aggttcaaat gacaccgttt attagtgaca acgattatga cacttgtgtc aaaaaatcaa
     2701 ctaaattttt gactactgga aataaagtgc gattaagtat aaaattcaaa ggtcgccaga
     2761 tccagcgtaa agagtttgga tatagcttga tatctaaatt tagtgacgat ctgaaagagc
     2821 ttggtactcc agaaggagaa gccagactaa tgggcaagag attgctcttc actctttcgc
     2881 cgatagctaa gaaaaaaata attaaaacag acaatgaaaa aacctagcaa aagattaaga
     2941 atcagaaaat cagtcagtaa tcgttttgaa gttaccaaaa acggtattgt acttcatcaa
     3001 tcgtctttta accgacattt acgtcgtacc aaaagcaaaa aacaacttcg tcgtttgaaa
     3061 ggcaagcaac acgttctcgg tcgttttgcc gttaaggtta aacaaatgct cggttttaaa
     3121 taaatttaca actttataaa ctaaattatg cgcgtaaaaa caggtacagt ccgacaccaa
     3181 cgtcacaaaa aagtcctaga tcaagctaag ggtttttgga tgactcgcca caaaagattt
     3241 aaggtagcca accaagctgt catgcatgct ggtaattatg cttatgaagg tcgacgtatc
     3301 cgtcgccgtg atatgcgtaa actctggatc attcgcttaa acgctgctct taaacctttt
     3361 gagatccgtt actccgcctt catcaatatg atgaagacca agaaagttga attaaatcgc
     3421 aaaatgttat ccgagattgc tatcaacgat aatgctactt tcaaatccat cgtcgccttt
     3481 ataaaataaa gcacggggga tttaatttaa aaaattaaaa gctcccgcaa gggagctttt
     3541 taaatatgat aaacaaacaa aagtatctag acaaaataaa ttctgccact accgcagccg
     3601 aattagagtc tgttcgcatc gagattctcg gccgacaagg cctcttaaat caacttttct
     3661 ccgatatcaa aaatcagcca aacccaaaag aatacggaca agaactcaac caactcaaaa
     3721 aagaactaga aaatttaatt aaatcaaaaa attttgaatc aacaaaacta caatctaaca
     3781 aaaaattagg cttgcccacc gtagctttag cgaaggtggg ccatttacat ccaatcacca
     3841 tcaccgaacg tcaactaaac gaagttttcc gcaaattagg cttttccatc tataacggcc
     3901 cagagatagt tagcgccgaa tacaacttca aacgtctcaa tgtccccgaa aaccatcccg
     3961 ctatggatat gcaagacacc ctctacattc aagaaccaga ttatttactc cgaacccaaa
     4021 cttcaactat tgaatcttat ctacttcaac aagaaagcca aaaccttccc attcgtgccg
     4081 ccttcccggg gagcgttttc cgtaatgaaa aagtcaatcg tagcaaccac tttgtttttc
     4141 accaatacca agctgtcgtc gtcgataaaa atgtcaccat gaaagatcta atcggtacca
     4201 tggacttaat gttcaaagcc ctctatggac ccgaagtcgt cgtccgttat cgctgtaaat
     4261 actatcctga agttgaaccg ggagtcggcc ccgatatgca atgttttagt tgtcatggta
     4321 aaggctgtgt tttatgtaaa tacgccggct ggattgaaat gggtggcagt ggcatgattc
     4381 accctaaagt tttaagaatg gccggtatcg atccaaatat ctattctggc tttgcctttg
     4441 gcatgggtct tgaccgctgg accatggctc aaaaaaatat taccgatatc cgcaccctta
     4501 tgggtggcaa tttagcctac aaaccaaacg aactatgaaa ttaatataca gtcatcttca
     4561 aaaatttctc cccgatctaa acgtcgaacc acaaaaactc cgtgacgatc tgactatggt
     4621 tggccacttc accaactatt acgaagaaat tgaaggtgaa attgtttttg acttagatat
//
LOCUS       DDWP01000104            7346 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_24837, whole genome shotgun sequence.
ACCESSION   DDWP01000104 DDWP01000000
VERSION     DDWP01000104.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_24837"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         2331 a         1300 c         1424 g         2291 t
ORIGIN      
        1 atataaacac taagtagttc atcaggacta tactaattgg cccccaagat tggagtgatt
       61 ttatagaata gtcccagtag agccacccta actgtactat tgatagtagt accaaccatt
      121 tgacactggt tatctttcgt atccaaggga aggctaaata aaatagtagt aaagagggga
      181 taaagtacaa ttggtaagct gcccctccga ttaataatat taagaaaaaa ttatttgggt
      241 tgggggcata aacaaagaaa taatatattg cactccaaaa tatatatggg gtcaatattc
      301 gactggccct ttttttataa aagtccaaag cattcccatt atagccatac tccaggacaa
      361 atccagatat cataataaat attgccaccg ctccccgggt aatctggtta aaccagaaag
      421 ttagaggcat accgagaaga tcattgtttc cgacccccaa agtcttacta gaagtatgga
      481 tcactatcac cattaatatg gctactatcc taattacatc aattgcatgt acataagtct
      541 tcttcattct taatgttatc attacctact atgttctttg gttacctatc tattttccta
      601 tttgcttttc tatcgactct ttcttttgtt aggatcaaga ctggcaagaa gatcgctgaa
      661 tttgtctata attttggatt tctcataggc tctttcgtct gggaagatat gtttatcttt
      721 tctatctatg gcctagttgc tagcgttgct actacttggt taggccaata caaatatggt
      781 ctacttttct ttctagtttt ttggatagtt agaagtgctg gagagacgct ttattttttc
      841 ttagagcagt ttattgagcc caaacatttt ccccattatg ttgacgaaca ttttcgtccc
      901 cttcgtaaga tttttggcca catctcctat caacaatgcc tcattattat gcaggtcacc
      961 tttcaggtta ttcttatgtc ttccattgtt ggattgatca cccttctctt ttcctgggat
     1021 aagttttaaa ataccagaca accatgaaaa caattgttat cggctgctca tcttccggca
     1081 agactactct tgcttctcgt tttccttctt caattgatat taacgaagaa tattctgaca
     1141 atattcctaa agtaatcgac gagactctag ccaagaaaga tgctttgctt tttatcaata
     1201 ctgattacat catgcttgat gatttacgta aagccaaatc tctgggcttt aacatagttc
     1261 aactcgagct taccttatct gaactacaaa aaagaaacac caatcatgat ttagttcagt
     1321 ggcttgaaga tatgattgat tatcagaatg aaattaagag agttggcctt gtggacaaat
     1381 ccctagacgc cactctacca gtcgagcagc tcgccaaact tttgtaacaa aggttgttat
     1441 aatcaatact tatgtctctc attaccgctc acggtcttca atatctccga cagcatttaa
     1501 ttgaactaaa acaggagctg gtcagaactt tcgatgaacg tgccgccgct gccgccgagg
     1561 gggacttaaa agaaaactcg gcttatattt ttatgactga gagagctaat gtactccaaa
     1621 gtcagattga taaagcttcc caggagatca aagaagctgt tctcattact cctcccactc
     1681 agtttaataa agttcaaatt ggccacctgg ttaagctagt ttttcaaaat gacaaccgag
     1741 aaatgactat tactttagtt ggtaaaaacg atgccagcca aaaacctggc tggatttctt
     1801 ctgatagccc acttgggatt gctatactcg gtaatagtgt tggcgacaaa gtcgacgtca
     1861 acggccagcc cgttgccatt ctcaatatct ctgctggaga tatataatag gtcatgtctc
     1921 aaatattttt aactgccgct gccaatcatg ttttgtcgga tcttgtaact catttacctt
     1981 cattaccttc cgaatataaa gttgctttta ttccgactgc agctgaagtt gaagaaggcg
     2041 aactttcatg gctcaaagcc gacaaagacg ctttaattag tcaaggattt gatgtcgaag
     2101 aattcactat taccaactta actccattgg aaattgaggg aaaactagcc aacaaaaatt
     2161 tgatttttat gactgggggt aacaccttct atcttttgga tcaaattata aaaactggtt
     2221 ttgatgagat tcttaaaaga aaactaaatg agggtgttgt ctatattggt tctagcgctg
     2281 gatcgatggt catgggtaag cgagtagatc tagtcaagac gattgatgat gcttcaaaag
     2341 ccccagattt aaaatcaaat ggtttagaga ttgtcgattg cgctttattg ccacactggg
     2401 gtgatacgca tttttttaaa ggatattatt tagggtttga ggctatgtat aatgaacact
     2461 tgaaaatgat tcctttgtcc aatagccagt acctttggtt taacgacggc cagttaaatt
     2521 atattgacgt aaaataaatt atgcaaaata aagtcgttgt catcaccggt gctagtcggg
     2581 gaattggttt ggctactgct aatgaatttt tgaaaaaagg atatcgagtt gttggtactt
     2641 cgacgacagg aaactcttca acctataaat tagacttatc ttcagagcaa tctatcgagt
     2701 catttgtcag gcagttaaat gaagaaaata tcagaataga ttttcttatc aataatgctg
     2761 ccattatgat tgatcagaaa gaaacttcgg tagatataaa gaaattaaca caaaccttaa
     2821 acgttaacct tattggcaca attagtttga cagaaaaact gttgtctata ttcaatgatg
     2881 gtggcgctat tgttaacctc tcctcagaat taggatcatt gcttcaaaat atgcccaaca
     2941 aatcttatgc ttatcgcata tccaaaactg ctattaatat gtacacactg accttatctc
     3001 aaagattgga gggaaaagga attagagttt atagttttgc ccccggttgg gtaaaaacgg
     3061 atatgggtac tatgaatgct cttcgtgaac ctcatgaacc cgctcgtgaa attgttacct
     3121 tgtgtgagtc aaacaataga tctggtctat tctatgaaaa agactctgtc cgtgcttggt
     3181 gatgagatat aatgtctata tgcaaaataa agtcgttgtc atcaccggtg ctagtcaagg
     3241 tttaggaaaa gaactctcac ttcagcttgc caagcttggt gccaaaattg ccctggtggc
     3301 aagaagtgag aagttgttgg tagaagtaaa aaaagaaata aatgacaatg gaggaactgc
     3361 tgagtatttt gtgtgtgatg tgactgatgt cgatgctatc aatttaaccg tatcaaaaat
     3421 tatggatact tttaaaacag tggatgtttt ggtaaataac gcaggcgttt ggactgctga
     3481 ttatttggaa gaaaaagata agaatctggt cgaaaaaact tttatggtca atagtgttgg
     3541 accaattaat ttttataaag cggtatctcc tatttttgaa aaaaataaaa aaggacattt
     3601 tgtatttatc aattcgattt ggggattaaa tttgcctgag acaaaagatg gattggtata
     3661 caccgccacc aaatgggcaa tgacaggctt tgctaatgcc ttgaaatata aatatggtgg
     3721 aactgatatt aagttaactt caatattccc tggaccgttt gtaagtaata ttgacaaaaa
     3781 cgcaggcgat gattttggtg acgatgagtc aaaagagatg aaaacaagtg aagttgccga
     3841 tgctgttatt tatgcattga atgctacagg caaaatgcaa attggtgata ttgaattaaa
     3901 atatacaaat tggaatagtt aaatgataat tacacagcca caagcaatca gagaagattg
     3961 ggatcaaact aaaagctgga actataaact caagcatttg agtccttatc agtccattgt
     4021 ctacgctgaa atcaccaatg atcacggtga agtacacacc aacgatattg aaagaattta
     4081 ttacatcatt gatggagcag gggagtttgt tattggtgat gaggctacca aggttgagaa
     4141 aggagatgtc tttactgtcc cagctcgtac tatttataat tatcattctc ttccaaatca
     4201 aactctaaaa gtattattaa taatggaact ttgggacaat taaaaattaa acaaatcatg
     4261 aactatcgca cacttggtaa aactggttta aaaatttctg atgttggtat gggcacctgg
     4321 cagctcggtg gtcatccctg gggttgggat gctcccgacg aagacgaaag cgttcgagca
     4381 ctatatcgtt ttgttgaatt aggtggaaat tttttggaca ctgcctgggt ctatggtcgg
     4441 gttgatgaag caggagatat ttctggaggt cattgttctg aagaactgat tggtcagttc
     4501 ttaaaagagt ctggtaatgc tggtaaagtt attgttgcct ccaaaattcc tccaaaagat
     4561 cgtaattggc cggctcgatt tggaatcaac atcaatgatg ttttccctag tcagtatatt
     4621 gaagatatgg tcgacagctc tctctcaaca ctcaagatgg actcaattga tattatgcaa
     4681 ttccatgtct ggcaagatga atttgcttct cacgatgaat ggaagcaaac tatccagaaa
     4741 ttaacccgtg aaggtaaagt caaacattgg ggtatctcta tcaacgacta tcaacccagt
     4801 aactgtttaa aaactttaga tactggtttg atttctacca ttcaatttat ttttaatatc
     4861 tttcatcaaa aaccgaccga aaaactattg ccttatgctg cagaacataa tattgggtta
     4921 atcgctcgtg tacctcttga tgaaggtggt ctaactggga gaatcactct cgattcaaag
     4981 tttgacgatg atatgcgctc tgtttatttc aatcaagaaa gattgcccga attcattagg
     5041 agaaccgacg agttaaaaaa aatattgggg gacgaagcca aaactttggc tgaattagct
     5101 ctaagatata tattgtcttt tgatgctgtc tccactgtca ttccgggcac ccgtaaagtc
     5161 gctactgtcg aggctaacgc tgccgtttcc gacggtagaa agttatctga agcactaatg
     5221 gttgagctaa aaaatcatgt ctgggagcgc aacttttacg tcgacagaga tccatcacta
     5281 gaatcaagcg gttttattga aaaataactt tccgggttat aatcagctta tgaatataaa
     5341 cgccgatacc aaaatcatcg ctcgtctcca tactaaggct agcccacgcg gtcttaacat
     5401 ctataatcct tttttcgagg aggccggtat aaatgctatt tatgttttgt tttatgatac
     5461 agactcttcg aaattaattt ctggaatcag aaatctgaat atggccggtg ctattactgc
     5521 tggttttgag tctaacgatc aactgccgaa tttgcttgat gaattagatg atgtcagcaa
     5581 atacattggt aaagtaggtt ttgtaaaaaa tgatcatggt aaatttattg gatcaactca
     5641 aggaggagaa ggtctattgc gctcgataca aaatatttcc aacattgctg gtaaaaaagt
     5701 tgttttgatt ggtggtggta atatcgctaa agcattgttg tttaatataa acaaattaga
     5761 ttcaaaacct tccgaagtcg tcatcttcaa tcgggacatg tccaaacttg aaaaactgaa
     5821 agccgacttt aactttattt ctgaaattaa gccactaaca gacttacact tagagtctgg
     5881 tgatatctta ataaacgcta ccgatattgg cggtagtgaa gaagatggtt tgtatacaga
     5941 gtctatcgtc aacaacttta atcaaattgc cgacgtcact ttcgaaaaag aaaatacaaa
     6001 tttagtcaac ttggctcgta aattaaataa aaaagtttct actggttggg atatgttcac
     6061 ttttcaggga caggttattc tagaatccat ccttgagact actatcgacc cggttttact
     6121 aaagaaacat gttgttaatg gtttgtcaga agtagtggtt taatatgcaa aatcgtgttg
     6181 gccttggaac atttccatta gttggtgttt ttaaagagat tgataaagaa tctgctaaaa
     6241 acattgttcg tcagttcatt aatgaaggag gttattacct agacactgct ccgaactacg
     6301 gttttggcta tatggaaacc ctgttgggag aagtcttaaa agaatttccc agagataaat
     6361 attacgtcgc caccaaatgt ggctatattg atgttgaaga taagacattt caaactttgg
     6421 ttaaaagtag taaatacgat gatgttatcc gcgagtgtga tcgttcttta aagaggctaa
     6481 atatggactt tattgattta tatatggtcc acaatcccga taaaaacact cctttcgatg
     6541 aaaccatgag ggctctaacc aaactacgag atgaaggcaa gatcggtaaa atcagtgtat
     6601 caaatgttaa cctagatgaa ctgaaagggt ataacttaaa tggagatatt tcttatgttg
     6661 aaaaccgttt taatttaatt agccgaagtt taagctctga gtttgaaaaa tatctactcg
     6721 accacgatat taaactaatt ccttaccacc ttttggatat cggtatgtta accggtattg
     6781 cctttgaaaa ctataagtta aggccagggg atatgcgtga aggtttaaaa tattggagtc
     6841 aagaaaatca agacattatt tttgcctggg tacgagaaaa gctggcacca atttccaaaa
     6901 agcttggcat aactattggt caacttaata ttgcttgggc attaaaacaa aaatttattg
     6961 attttgtaat tgtcggtact accaatccaa aatatttaac gattaattta aatgccaacc
     7021 agattgatct ttctttggaa actctagaag aaatggaaaa aatatatcta gagttagaaa
     7081 cagacatcaa aaataaattt ggaaaatcaa ttagagaatt ccgtggtttt aacgaaaagt
     7141 tttattaatc cttgtagaga cgccatgcat ggcgtctcaa tttaagaatc aatttattca
     7201 tcaaaaaacc tcccgattac tcgggaggtt ttgagtgtta ctagaaatag attagtatct
     7261 atcgccacca ccaccataac caccttggtt accaccaccg aaaccggtgt tgcgtggacc
     7321 acgggaatag ttatctcttc gtggag
//
LOCUS       DDWP01000105           17351 bp    DNA     linear   ENV 22-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA2283
            UBA2283_contig_38419, whole genome shotgun sequence.
ACCESSION   DDWP01000105 DDWP01000000
VERSION     DDWP01000105.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06452308
            Sequence Read Archive: SRR5324075
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA2283 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA2283
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 17351)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17351)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 56.49x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17351
                     /organism="Candidatus Shapirobacteria bacterium UBA2283"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA2283_contig_38419"
                     /isolate="UBA2283"
                     /isolation_source="biofilm"
                     /db_xref="taxon:1953383"
                     /environmental_sample
                     /geo_loc_name="Canada: Northern Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559898 metagenome;
                     metagenomic"
BASE COUNT         5021 a         3586 c         2842 g         5902 t
ORIGIN      
        1 tagaaaggtt ggtaaaagag cacccaactg taataatttc tcatgttgat gattcaatac
       61 cgtatgggtg gacaagtcgc agaatacctg aagaggtgaa atatatgagt agcattggat
      121 acgattcaga agtaattttt caagggcaga ctgacaacag gcaaaccaca gtcattcaat
      181 attcaccaac tcaaaaatct taacggtgag tcttacttta aatatactga acattcggta
      241 aaatagttct aaaaccgtcc caccctgcga gcaaaccaaa acaccattgt taagtattta
      301 tttacctgtc gtagtcgtat caggtttctt tatttcatga cgaaggtaga gccgaaagta
      361 ttctggctca cctttctttc ttcttaccag acatggaccg tctggaggat tattaacgac
      421 caactcgtta ttttcgatta ttattggtaa atcatcttcc aggttattga ctatccactc
      481 tgcgtattca ggatctatcc ctttatttat ccattctttg agatcttttg tcgagctata
      541 tccctggctt ccttctggtg gatcaagttt gaatacggta ttgacagtaa atgtactact
      601 atctggatta tagtttatag ttgtagatga tgaatttcga gcggagtcgt caataccaac
      661 ccccgcgata tgaacagtgt cagatccttc tgctgtaact gtaaatgtgc tatttaccct
      721 accggtaaaa aattttgaat aaccacctct tgcgtcttct tcatgattaa tggagaacga
      781 gagatatcca ctgtctggat ctgggttatg gacaccttca actaccagct caccactgtt
      841 aaccaggtta ccaatatcgt caacttgacc cataactact tggatgctgt gtcttgtgat
      901 tggatctacc tgacctgcta tcaagttaat attttctaaa actctaccaa gttttctata
      961 ttcattcaaa agtttttctg ggtcaatgcc agcactatac ggataacttt cttttactgg
     1021 ttcgagccca attttgttta ctgggttatc ttctcctgtc gtaaaagcgg taggcattgg
     1081 tgctgtgctt ttgtctagtc cactcatact tataatttaa cacgttaatg ctcttgcttc
     1141 ccagtgttaa gtccagaact ttgactttct cttaatagag gtaaatataa aagttctatt
     1201 aaaaaagtaa tataatcaaa ctaacaatgt tttgtttatc ttgtggtaaa aaaatatcta
     1261 aaataagtaa gttttgtaag tattgtggct caagtcaaat aataaaaaag aaattaaata
     1321 aaaaagaata cgaagcagtt tggacttgtg attattgtgg caaagaattt aaaacaaaag
     1381 ataaatcaga tgaacacgag ttaacctgta agaaagataa agataaggac acattattta
     1441 taacagagtt ccccaacagg gaggcagttg tttttctagc ctctatgttt ttaggaatat
     1501 atttatttac ttttgcagtt gttaattcat atgcaaaaac aaacggtcta gagaaaaagt
     1561 atttaaccaa ccctttaaat tggtttttgc ccgagtcaaa agataaaata atttcaacac
     1621 ctacaattac aattgctcct ccaccaaaat taactccaac atctaatcca aaactaacaa
     1681 cacaaacaaa taacacaaat aataatcaaa ttgaatgtat aggaccagac ggaaaacatt
     1741 tcaacacttc gatgtctgag tgcgaaaagt taaataaatc ctggggacat aacaccgatt
     1801 atctagtcaa ttgcaatgtt agtagtaatt gtggaggtgg taccaagtac ataagaaaat
     1861 cggagtgcga taattccgta tgctgtcaga ttggtagtaa ctggattctt tatacttcaa
     1921 aacaaaaatg tagctcggat caaactaatc aatataaagg aaattatact tacccaactt
     1981 taattccatt ttccacaatg aagccactcc cgacatactc ataccaggta ccacaaagct
     2041 atgcatctcc gacgatgact gatgcacaag cgcaaagttt aattgatcaa cacaactcac
     2101 aagtttcatt gtgtaggggg caggtcgttt ctaagttcgc tggattaatc caaagttgta
     2161 atcaatatgg tggttcatct gcggcagagg cttgtcgatc aatctatgaa aaacaaagac
     2221 aagcagaata caacagttgt ggacagactt actgatataa gtgatattat tatataatga
     2281 gtccgtattt tccaacagta gttttacttt tatgtttctc agtcgctgtt aattttttct
     2341 tatggctcat aatcgactca tggaaaaagt actctttgtt tttgcttaat aaaaataaac
     2401 cttttgtaaa gaaaaaacga cttatgtctc caaatgagtc aaagcttttt ggcatcttaa
     2461 agacatttaa tacactcgaa gactatgaca tttttcctca agtcccatac tcgtcattaa
     2521 tggacgttga tcccaacact tctgacttag gaatgaggtt tgaaataata aataattaca
     2581 gatcagactt tgtacttacc aataaaacag attcctctgt caccttagtt atagagttaa
     2641 atgatcaaag tcattcttat aactataaaa aagcgagaga ccttttcgta tactcagcat
     2701 taaaaacagt aggaatccct tgtctggttt tagaaaccaa agacttggtt aagcctagca
     2761 aaattgaaga gagtattgtt gaaaaactga gtaaaaatag taactcaaaa taaatttgag
     2821 gaaagaataa ggaagtactt caaaaataaa tataattggg agatgactag cgaagagtat
     2881 gaggagacat atcaatcgct ctttcatcta ggtaaagcca tatctttatt taatatacag
     2941 attagtaaga aatatttcta aaccgtccca ccctacgagc acttcacttt gcacacggaa
     3001 cttatctata ctgctcatta cgtagaaacg acctcgaact tttgatactt tgataaaatc
     3061 attttatgaa aactaaaaat gatgataagc tcacaaaatt agagataaca gttcaaaaga
     3121 caacagaagc aatcgccaga atacagcgtt ttgttatcaa atacggttct gacttaaaca
     3181 acaatcaagt ggaaagaata tcaaaaaaga ttcatgaatt acaggagaaa gtttgtagtt
     3241 taggtaaata atatgaataa agatcttatt gctccctgtg ggatgaactg tggtctttgt
     3301 ctccactatt taagggctga taacaaatgc ccaggttgtt ctagcggtag aaaagttaac
     3361 ggcggatgca ttaaatgtgc tatcaagctt tgtaaagaaa ggaagggaga atattgtttt
     3421 aattgtggca aatttccttg tgacagatta aaacgaatgg ataaaagata ccaagagaaa
     3481 tatgagatga gtgaaattgc aaacttgata gctattagag aaaatgggat ggaattattc
     3541 ctcaaacaag aagaagaaaa gtgggtaaat tcagatggga catattgtgt ccacgacaaa
     3601 aaacgatata aataaaattc gctagattcc tacaccgcaa aaattttttt aaatctaaaa
     3661 ttgatatact ctatcttgga ccaaagagct gagggtggac cgactaaaga ttttgtatgc
     3721 gtaccaatca gagagtattg aagggaatgt tgtagaaatt acgaaacact tattgtttgt
     3781 aaatatccga ctctaatggg tcaatccaat tttccaactc tctttcattt tgtacatacc
     3841 cccttaagtc agccgatttt ttattaatac caaaatgtag gtgctttctc tcaaaatcag
     3901 tttcctgtgt ttgtccctct cccaatatcc caatttgttc accccgtagt acattcagat
     3961 tttttgtaat actgctatct tttaaatgtc cataaatata ataattctct gcacatttca
     4021 aaattagaac ccctccatat cccgacaccc atttcgataa cacaatttct ccattacaga
     4081 cagccagtac tggcacttca ccaaccacgt cctcatattc cacatctatt cccgtatgaa
     4141 atcctttaaa tctttctggt gatacagggg agttctctaa agttatatat tccccaaatg
     4201 tctttttagt aattctttgt ctaaattctg caatcgggta aatacttttt acttcttctt
     4261 cctggctaac tgtcggtatt atttctacag tattattctg tatctcaggc tgtccctgac
     4321 gctttaataa caactgtgcc actcctaaga ttattatcaa aattattatc aaccaaaaaa
     4381 tatttttctt cacttttata ttttactaaa ttatccagcc agttatattt aatcttatta
     4441 tcgcgagtta taatcaactc atgaacgact aaggatactt gaagttagca gttgagcaag
     4501 atgccaaagg caacagtcca gcgactctga acttgaaaac acctctaatc aaatatacta
     4561 ttaacatatg actacatctg aaaaaatcaa aaacatgttg acttggggat ttatcttatg
     4621 gtttgtcggt tatcttggag gtatggtctt attttttgtt gtaccaaaag actatattgg
     4681 ctgggtactg tcaccgtttg ccagtatttt taccatctgg gttctaatga caaaagtcag
     4741 gagaccagag ctaatgtgct attttggtac aggtctaatt tggacaatca tggccatagt
     4801 cctcgactac atttttattg taattttact caaaactggc agttcttatt acaaactcga
     4861 tgtttattta tattattttc ttactctagc catgcctata ggagtaggtt attggaaatt
     4921 taaacacaag gcagtaggtg caaaattgtt ttaactaata tgtccacctc tccatcaact
     4981 attgatttta ttttggatca actagtcgat gttggtaacg tgtcggcccg caaaatgttt
     5041 ggtgagtacg cactatattg taatgagagg gtggtggggt tgatatgcga cgacactttg
     5101 tatttaaaaa ttactgaccc aggtaagaaa tttgttggta aatattatca agagggcagt
     5161 gcttatccag gtgccaaagt ttccatgatg attagtggtg atttgattga ggatagtagt
     5221 tgggtcagcg aattagttaa gattactgcc gataatgttc cacttcccaa gcccaagaag
     5281 gccaaagtat aaaaatccaa gtcatcgtcc caggttgtcc tgcttacaaa aacctttatg
     5341 aattaactag aactgctgtg gtagcattac ttgttaatga caggttctag aaaaactatt
     5401 agctcgtaac tcaattacca tggatatttt ttacccaatt cagttcttag ccgataaaat
     5461 ttccttaaat ccggtcgtca atttttttat ttatgacact attaaaatag gagttctctt
     5521 gttggtcata aattatttta tggccgtcac tcgctattac ttcccgatta ataaagccaa
     5581 aaatttcctc gcctctcgtc gatggtatgg tctcgactat ttattagccg ctttattcgg
     5641 cgtcgttacc cctttctgtt cttgttcctc cgtccctctt tttattggat ttttaagtgc
     5701 tggtatacct cttggagtta ctttttcttt tcttatcgct tcccctctag tcaacgaatc
     5761 atctctaatt ttgttccccg ctctttttgg ttttaagacc accgttattt ataatctcgc
     5821 cggtatcatt atcgccatta ttggtggaat aatcattgag aaattacatt tggaaaagta
     5881 tgtcaatcca gacttattaa aacacccagt tatattaaaa tcacaccaaa aaacacgaaa
     5941 acaattatta tttctgtggt ggcaagacgc cattaaaatt actaaaagta tttttcctta
     6001 tgtcgtgtta ggcgttgccg ttggtgccat tatccacggt cttatccctg atgctttttt
     6061 tgaaaaatat cttagttctc gcgcttggtg ggctgtccca ttggcaactt tattaggagc
     6121 tccattctat gctaattcag tttctgttat tcctattata gaagcttccg tcggtcgagg
     6181 tatccctctt ggcaccgctc ttgccttcat gactgccatt gttactctct ctattcccga
     6241 agccctgatt ctcaaaaaag ccatgaagtg gcaattattg atcacatttt ttggtatcac
     6301 catcgctggt atcatattca ttggctacct gttcaactat ctttcgtaga cactctctat
     6361 ccctttcacc tcattccaat cttgcgagct ggctaacaga atagtgacac ttgtctaaag
     6421 taaactcata acaattaaat actaatataa attttatgag ccaaaccgtt tacaacaccc
     6481 ccgccgatca atctaatcaa aataataata tgatttggat gattgctctt ggacttgccg
     6541 ctgttatctt tttcttagtt gtggtctaca gtaatggaga taattctggt aacgaaatgt
     6601 cacctaccca gattccacaa agtcctcaat ccactcaagt tcctcaagaa ccacagagta
     6661 ctcaagttcc acaagagcca caaagtactc aagttcccca gccaacgaca gttgaggcaa
     6721 ctgcagctcc taccattgca gttcaattat aaattgttct ctaaaataat ataattatgg
     6781 tcaaaataaa acatgtcaat caaaaattaa aaggaaagtt acagcaaata aatggcgata
     6841 ttcagattgc caataaccag ccagtcaaag gaaacatcaa caaagttaaa ggcaaaatga
     6901 atgagaccgt agctgatgtc aaaattaaaa ttgacaatca aaacaactaa taccgttttg
     6961 ccatattctg tccagttttc taagtctcaa ccctgatatc aaattctttc aataagtttt
     7021 tcatcttatt taactgatag gggaaaacgc agtacttatc aatttctcta gggtatatcc
     7081 atttaactgc tttaatctct tttttctgtg gcaaaatttt tatcccttta gtcatcttaa
     7141 ctaaaaactg atttctttcc tgtccgcgaa atggtggctt accttctttt atcttctgat
     7201 ttattaattc gtctgaccat tcgtaccgat ctatctgctc actaactttt aatatctcac
     7261 aataattaca tcccaattct tcacttaact ctcttataac tgcctcgctc ggtgtctcgt
     7321 tttcatctac tcctcctccc ggtagatccc attcgttatc ctgccaattc attttttgta
     7381 cgagcagtat ttttcctaaa tcgtcaacga tcacagcact agtactctgt ctgtaattta
     7441 atttattacg gtcaatttta tctgcaacgt tcattttgta cctactttat attcttggtt
     7501 acttcttggt taaccgcaat atacggatga gcatttattt tcccttcttc attttttgta
     7561 aactccatac caatctgcat agcccgtagt gactcttgtt ccatctggtt agcttgctgt
     7621 tcagcataat ttaggtggtg tcttgtatta gtttcaaacc attctttgta aaattcttta
     7681 tattctccat ccaattcttc gtctgacaac ccgtagttag atattaatat ttgtcttaac
     7741 gtcaactcaa ccagtgtcgg gaagtctttc atattttcta cataaacagc ggtaaactta
     7801 ttttttcttt cagtgtcagg cagaagtgca acgctacacg cctcctcgac ctcatacttc
     7861 agctccgttc ttctgtcagg ttcgacttct cttagtatgt attctggata aattagttta
     7921 attagttgat tctttattat cctagcttct gcctccattg gatccgcgtc atgttcttcg
     7981 atagtttgta cactatttgt accatcagtt gatttttccc cccatatttg aaactgaaca
     8041 aaatgtgtca tctcttcgat catcgtaatt ccagctacta tctcaggatg gtccaacacc
     8101 tcacttttta ggattacgtc acctggtttt gtaattgctc gggaatatgt ttgagtgagt
     8161 tgtccttcaa gttgatttat cgataaggca ataacattgt tgcccggatc acacattgcc
     8221 atggagtgat ttccggaact gtccaatatc ggaaaaagtg tataaattat tacaggtata
     8281 tcagagtact taacacctag ttctgttctg ggaaaagtcg aagcaccttg ttcgtttctt
     8341 tcgtccatgt ctaatagatt aaacggactt attcggttct tgtatatgtc actcggaact
     8401 ctaaggtagc tcatcactag cgaagcaact cggtttgatt cagaaattac ctcttcatta
     8461 aaaagtccat ctttcggacc aaatctaaaa aagggaattg aaatattgcg accagcttcc
     8521 gtctctgata gcggctggat attgtgtatg tcgacagtat attttggtgc ctgtgtcttg
     8581 ccatcatcaa tgcgtttatc caacatacag ttattataca gacttgtact cagaaaacta
     8641 aaacctataa cattgccacc agacgctaaa agattttggt tcactacttt tgaaatttta
     8701 ataggcatag tcaacaactt tttcgagaga gttaatgctc ctcgataggt gtccataaaa
     8761 tatttaattg gagttaactt gtataatcac cttatgaaac tatatttatc ttcttttcgt
     8821 tttggtgatg agccacaaaa actagcagat ttagtaggtg ataataaaaa tattgccatt
     8881 attccaaatg ctcttgatat atatcccgac agtcctagaa aagacgccgg tttgcagagg
     8941 gagacagacg gtttattaga gctcggcctt cagccagaaa ttttggacct aagagattac
     9001 tttggtaagt cagaggagtt aaaacaaaaa ctaagtcaat atggagctgt ttgggttttg
     9061 ggtggtaaca cctttgttct tcgcagagct tacaaagaaa gtggttttga caactggtta
     9121 caacaacaaa aagacaacca agactttgtc tatgctggct acagtgccgg agtatgtgtt
     9181 ttgtctcctt cactaaaagg tctagaaaca gtcgacgatc ctgttttggt tggtgacggt
     9241 tatcccacag aaactgtttg ggatgggcta aatattatcg attttgcttt tgctccacac
     9301 tttgaatctc ctggtcatcc cgagactgaa atggttaatc acgaagtcgc ccattacaaa
     9361 aaagtcggaa taaaatacaa aacacttcat gatggtgagg tcatcattat ggaaatttaa
     9421 gtagttaaaa cctcattact tatcaatgtt cccattgaaa aatctttctc cataattacc
     9481 tttggtagct catctaggtc ttttatatca aacacaaata ttggtaaatt ccattcttcg
     9541 catttagtaa aagccgtcat gtccatcact ctcaactttt cttcaaacac ttttttaaag
     9601 cttatttcag aatatttttt tgcatttgga tttgtcttcg ggtcacggtc gtaaactccg
     9661 tctgttgtac tagcttttag taccatctga cattttagtt cccaagcata ctgagataca
     9721 gctgtatcag tcgaacaccc cggtaatcca gttcctccag ctaatatcac cattctaccc
     9781 tggtctagat gatatcgggc cttttgtctg atataaggtt cggcaaagct gggtagagga
     9841 aaagccgtca tcagcctagt ttccacttct ccgtttctaa tcaaagcctc tcttagtcca
     9901 attccattca ttactgtcgc caacattccc atcgcgtctg cttctgtcgg atctacctcc
     9961 tcatttgcca gtctacctct aaatatatta cctccaccca ctaccaccgc catttctacc
    10021 ccagttattt ttttaatttc aatcagtctt ctagccacct gatctaattt ttcaaagctg
    10081 atactgtctc cattctgtcc aaataattct ccacttgtct ttaaaattac cctgtcaatt
    10141 tttttcactt gctagattat aacccatttt aatgtttaat tatgactacg tctaaaacaa
    10201 tccttctttc aactcatcca tagagaactc gacttcaaaa gttgttcggt caccgacatt
    10261 cacatacaag gtcaatactc ctgctgttcc ttttttaatt attcccccac acgaatatac
    10321 cactcggcgg atatccgggt gcaactcttc tattagtgct tttcccatat tgtcaacaaa
    10381 agtgttcggc accaatattg gttcaggtgc tacttcgata ctaaaaccat cctctgtccg
    10441 acttaactta gcccttccga tactgtaaat atatttatcg actccatcaa ttttttcaat
    10501 agtaattccg tgaataataa ataaggcctc tttgtcgttc agccatatcg gtggcattgt
    10561 tgtccctatt cgccactttg cccatgtcag atccgtcgga aatactatat caccgatttt
    10621 ttctggagtc tgtccgtgga ctgaaaatac caaaatcttg tgattataat cagtgctatc
    10681 tggtcggaaa aaataagttg tattatctac tggagtcata tttttcccag gaccaaaagt
    10741 actcactata agaattggcg gtagttcttt ttcccaagta tggtgagaat taatctttgc
    10801 cactgctgga aatgggatag gtcttcccat tttgtcagac aataccgccg tcaaccctat
    10861 taccaaattt tcattttcta gttccaatgc tctcgggtcc tccaagttta ttccttcata
    10921 catcggcacc cagattgtcc tttcttggac tacttcaccg tcggctccta cttcaaatag
    10981 cttcaatatt cccttatctg gttcgccatg ttcactcgct ttttccactt ctctgccaat
    11041 taaatacacc gaatcactgt tgtgtttttg ccaagccgcc aagttatatc tcgacacttg
    11101 taagtttgga aatatattcc taattttttg aattttcatc tgcttccatt tcggttatta
    11161 ttagtaccga tattaaatac gacagtaacg attcggctcc ctgattttta ttaactcctt
    11221 ttatcgttag tccatcatag caccccccag tctcaaaatt gtaaataggt acattcttgc
    11281 tgttatttcc caaaaaccag ctaaaacata ttctggctaa atttttatat ttttcgcaac
    11341 ctgttatttc atatgccgtc tttaatgcca atatcattga ggctggatct tcaggctgtt
    11401 ggtcataatc accaccctcg ccatctttat taagccatgt ttgattacct gtcgggtgat
    11461 aagcatcgtt attaaaagta ttttcaataa gataacccag tgatttttca gccacatttg
    11521 tgtatctctc attttgaaaa tactttcccg caagatatag actctctggc ataaccccat
    11581 tattatattt caaccctttt tcaaaccact tgccatcgta ggccatggtc aattcatcgg
    11641 catacttttg gacaacctct tccatctcat ttttgtgttt cggatattgt tttattgcca
    11701 gaagtaaagt ttttatcatt attgccttag ctctcttatg ttctctagca tcaaaatttt
    11761 tccagtcatt ttcaaagatt gtttttgcgt ttttattatc tgccatcact tctgccaatg
    11821 cccatagtgt tctcgcattt gcatctgtta aatcctctgt ctcattttgc aaagtaggac
    11881 gtttgtcttt gtagccaatg tagtttacaa aaccaccatt ttctttttgg ctttttttaa
    11941 tatattcaag atagatttcg gttaatttat ttatctttaa cttattactt attatcaaag
    12001 cccgagcatt atcatcgata gtataaccaa aatcaggact tggtttttcc ttatcggcaa
    12061 actggaatag gccaaacttg tcagtcattt taattaggtg ttttagattc agagggggga
    12121 taacattttg agttaacagt tttatacatc ttaatgccac gttgtcccat agcattggtc
    12181 ttgtcatttc ataagcccgc ttgttcatca atagcagttc tttatcgtcc atcattattt
    12241 cattcaacgc ctcactcatg gccacagaat cctttatcgg tactagtctg ccaacttctt
    12301 tactgattat ctctttcgcc tgggcaaatt ctgtactgac tgtcgcccta cctgttccca
    12361 gtgcgtagga caatgtcccg gatactgcct ggtcagtgtt tattgatgtc gagatgtata
    12421 tatcagtcgc tttcagaaat tcgagcagct ctttgatatg tagatattgg tcatagaatt
    12481 tcacatgatt tttaagattg agttttgtca ccaattttgt taattccatc cggtaacttt
    12541 ccccctcttt tctgcgcact accgggtgtg ttttccctag tattaagtaa cgaacttttg
    12601 ggtgtttttt gactactgct ggtaacgcct taatcacata ttcaattccc ttacttcgac
    12661 tcagtagtcc aaaagtgctc agtattttaa aatcacccaa cttcaatttc tttttactat
    12721 tagctgtagt cgtaaatttg gttgggtgaa tcccatgagg gatcacttga atttttccca
    12781 ctgattccag atacaatttt tccaacactt tttttgaact ttcagttaac actacgattg
    12841 ctttaacgtg ttggataatt ttttgggtca cttcaaacat tttttcatca ggtgttggta
    12901 aggctgtgtg aaaagtcgcc actattggtt tcttgcatct ctctattaga tacaaaatat
    12961 tttcacccca ctcaccacca tatattccat attcatgttc tacaataagc ccaatacatc
    13021 ggtcatctga atttaacttt tttgccaaat ctatatgttg ttttttattg ttctggtcta
    13081 tttcccaccc aacttcgact ggatacacgt gtttgtctag tggggacaca ttcatagccg
    13141 caactttaca ttcaatacct ccattcaaaa tagatttaga tgaattcaat agatcttgag
    13201 taaaagtcgc tattccacat tctcttggtg ggtatgtccc tacaaatact atatacggaa
    13261 tcttcatcga tctattccca ttatacccta cgtatttttt ctgtaattct cgggcaccac
    13321 atatatattg caatggggat tacataaacg attcgtgcca cattactgaa ttgttagaca
    13381 gttaaactca tactaaatta tatttcctat gaaacttatc aagacaaaat tcaaaaatgg
    13441 ttttatctct ccaaaactat ttcttcagcc attagcagcc aacaccaaac atcactcatc
    13501 tagtttgtta tacaaattat atcgacacag tcctttttgt gacttttgga cattattatc
    13561 tacaccaata aattaaccta aaatcaaaaa aaattgctta agtcctatca tccgagtata
    13621 attaacatat caatccacca aacatccaca taagttctga caaaaccaaa gaattcgaaa
    13681 ctttctacga acaatttgtt acccgtaact acaaaaaagg cgagatgcta attcgtgccg
    13741 acgatgatcc tcaaggaatt ttttgtctca ccaaaggcta tgtccgtcag tatactattt
    13801 ctcctgctgg taacgaatta tcccttcata ttcttaatcc tatatcttac tttcctatgg
    13861 tctgggctat caatggtact cctaatgtct atttctttga agctttaact gctgtcgaag
    13921 ttggccgcgc tcctcgtgag caagtcgtta attttattaa agacaagcct gctcttctct
    13981 ttgctctgat gagcgaattg ttagaagact atgccgagac tctcactcga gtcgaacatc
    14041 tagtttttag tgatgcttac cgtcgagtaa tttctatttt aatttatatt gccaaacatt
    14101 ttggtcaagt tcacaaaaaa ggtattatcg ttaatcaccg ttttactcaa gctgatatcg
    14161 ccacccttgt tggtgtagcc cgggagactg ccagtatcga gcttttcaag cttgaaaaag
    14221 ccggacttat caaatatgtt gaccatgcta ttctgtttga agatttagat aaattagccg
    14281 ccgagataaa ttctgttcct ctccagtcgt aatttccctc gtctctttaa tgtaagccag
    14341 cttacaccca caccacctgt agcattactg tgtattactt gaagtatatg tagactacta
    14401 agtagttcaa ctatgtctac tgttattcaa aagcaaagaa ccactctgtt tataaatcac
    14461 tcgatagtga ctcatgccaa agctcaagct ttggtggaga acaccactct taccaatttg
    14521 gtagaaagag ctttagtggc ctttttgccc gatgagatag tcgtcaagaa accaaagata
    14581 tagttaacta ctgttaccag tttaaataat tcagcatatg gctactatta tcaatagtcc
    14641 cccctcccaa gacaacaatg cttacggagc attctttgtt attttcgctt tagtcattgt
    14701 cggtatcatc tttgtttact ttggcattcc tgccttaaga caaatgggta ctccccaaat
    14761 caatgttccc gccccagtca tcaacatgcc tggcgaagtt gacgttaatg ttcaacaaac
    14821 accgtaactt ttaataatta aataggagat aaaaaatatg aatattatac tttggatcgt
    14881 ctttggagct ttcgccggct ggatcgcttc gcttattatg aagaccgatc agcaccaggg
    14941 cacagtttac gatatcgttt taggtatcgt tggctctatc gtcggtggtt ttctcatggg
    15001 cctaattggt caatccggag ttactggatt taatgtcgaa agcttaattg ttgccgttat
    15061 tggctcgatt atggtcatct ttattggtcg taaattacgt cgccaatagt taaaaattta
    15121 taaaaatata tgacagataa aaatttaaaa actcaagtta acgatagtgc tattaatccc
    15181 gttgtcgctg ctgttgctgg ggcagttgtt gctggtgtcg ctgtcgctgg cgccatgatt
    15241 atgtccgata agaaaaatca ggacaaagtt gctaaagttg tctcaaatgt taaagatgat
    15301 gtaaatagca aaaaagcatt gattgtcgac aaggctacca agttagaagg cattaccaaa
    15361 aacgctgtta aagatatcaa aaatatatga aagaaataat tgttactcca gaagttgtta
    15421 gcacccccgt ccgtgtcggt gctaccaaca aagagaccac cgaatatcta atttattttt
    15481 tctttggatt agtggaagtc ctcttggcca ttcgattagt tctccaactc actggagcca
    15541 gcacccttag tggttttgtc cgtttagtct acggtattac cggaatattt gttctacctt
    15601 ttcaaggtat ttttcgtcaa gcctctgctt caggttcagt tttggagccg tcaaccatag
    15661 ttgccattct cgtttacatt ttattagctt ggggtatcgt taagctctta cgtatttcct
    15721 ctggcgaaca acaggagtca taaaaatatg aatatttctt ttgcttcttt aagtccttta
    15781 tcagccctag tttttggaat tttaatttta ctatttccaa aattaatcaa ttatattatt
    15841 gctgcctatc ttattattac tggccttctc ggtcttggta taattaaatg attatgttaa
    15901 atctgatcgt ggttcttgtc actagctcag ctttggttta tatttctggc ttcaattcca
    15961 tgcccgtatc agttaacttt gggtcttttg ttgtagccaa tgttccctta ttttatgtca
    16021 tcattgggtc tattcttttt ggcatagttt tatcttattt attccaccta atcagttctg
    16081 tctccactgc cctagcactt aggagtaaag acaatgaatt aaagaaagaa aagcaagaag
    16141 tcctcgagtt aactaagaga gttcatcagc ttgagctaga aaatgagaag cagaaaaatc
    16201 ctgacaccgt cattatcccg accgatccaa acgctctatg aaatctttat tggccaagat
    16261 ctataaagct cttcatttgc caaaaaacct tcaactttct attatgaact tgactcaaga
    16321 taggttcttg gttggagtca ctggagtgat tctcgatagt gacaacaaaa ttcttttatt
    16381 caaacacact taccgttccc attcttggag cttacccggt ggttatctaa aatcaggtga
    16441 gcatcctcgt gaaggtctcg agagagagat actagaggag tcagggttag ttgttagtgt
    16501 cgatgaatcg gttaagacaa ggaccgatcg agaattcgcc cgactcgatc tttgctatac
    16561 cggtgtatta attggtggcg aatttactcc cactcacgaa gtcacagagt acggtttctt
    16621 tgccgtcgat cagttgccat tattaagaca aaatcaggtt ttcttaattg atttagtctt
    16681 aaaatcttta tctccaaaag tttaaaattt gtctctatac tattaatatg gattacttag
    16741 tttttttacg tcagttcaaa gtcggtcctt ttgctatttt tgacaccgtc atctcctata
    16801 tcggaatttt tcttctggct ccacttttaa ccaaattatt ttccattttc aaattatccg
    16861 ttcccctcag ctcttggctt tggttgaccc tacccatctc tgttttattt catcttatct
    16921 ttgcccagaa tactcctttg atgaaagttc tggctgaccc tagtcacttt tctttctaca
    16981 tcgccgtcgc catacttttg ttgatgacct atatgggcct gaagagtgta aaattcctct
    17041 agttctatgt cgtttcagaa aatattatct cccctctatc cactgatcat caactatttt
    17101 aatgtgtctg tctcggggct agaaaatatt tcccctagta aaaattatat ctttactgcc
    17161 aatcatcaaa gtattctcga cgtgcctctg attttttcgc tcctcgccct ccggaccaat
    17221 cgcaaaatac atattcttat tagtcaccga ttttatcgtg cactttggtt gattactcgg
    17281 ccgttgggaa tgatctctat ccgtatggac aaaggcactc aatcatcgac cgatcacaat
    17341 cttcgcagtc t
//
