LOCUS       DHGB01000001           12312 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_54904, whole genome shotgun sequence.
ACCESSION   DHGB01000001 DHGB01000000
VERSION     DHGB01000001.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 12312)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12312)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12312
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_54904"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         3593 a         2476 c         1870 g         4371 t
ORIGIN      
        1 aaagtgcccc aaatgtaatg gccccgtcgg tctatctccg gtcagtatcc tttttttctt
       61 tcgattcata tatttatatt gaaattttaa catttaagat ggtcaataac tgcatcagcc
      121 aaaatatcga gactatcaaa aatttttaca tcaccaaatt ttgatttttg aaatgctaaa
      181 ggcagttcag tacaacctaa aataactcca tttaattggt tattatcaat aaacttcctt
      241 gtaaaatcat ttaaattgtt aacatccaat ttagatattt tgttatttaa tactccccga
      301 ataacctttt ctaataaatt ttgaaattta ttatctggaa tcaccggtat taaatcggac
      361 atcagcaatt tattttcgta caactttgtc ctcaaagttg ttggagaagc taaaattccc
      421 actttattga tttttttatt tttaatatct gttaccacta aatcaataat tgacggaaat
      481 ggacactgag caacttttaa caaatcggta tgcataatat gtgctgtatt acaggccata
      541 acaattattg atgtttcttg ttggttaaaa aattttattc gttcagaaat aatttttttg
      601 gcaggaacaa ttttgtttgg attagaaata aaatcttcaa tagcaattgc attcaaaaca
      661 atttcaggta ttgctaagtt ttctgcgcta attttttcta agagtaattg aaaaaatcta
      721 gccccggcaa atgcccccat cccatcaatg attcctattt ttttagtttt aaaatttttc
      781 ataaaataaa aagtaaataa taaaaagggg attatgcagg aacgaagaga gttggactct
      841 tattacttgt tctggagacg agcgttttgc caattaaact acgttcctaa acaaaaatct
      901 cccgaaaaac cgggagattt aatattttag aaaaaatagt tttatgccat taaaacttct
      961 cccgattggg ctactgtgta tcgccaatag aaagaacgtt taattaatgt cattgggtca
     1021 aatatatcac taacgtagcc attgtcaata gttgacctat aataatatat aacttctaat
     1081 atagaaaata tgccattaaa agaaattttt caaaaagaac ctctttatga tcctgttcac
     1141 gcccacatgc taggggttga cgaaaggtac acacagagaa gttaagaggg ggaatttatt
     1201 aatggtggag atccgatgcc aagatcagaa ccactaaaag agagtactat ttcaaaatta
     1261 gaatattatt gaaatagttg gatacaagta atgtgttata atcgactcta gttttattag
     1321 ccgttagtga agtcggttaa caaaggcgct aaggctaata atttttttat gaagaaagaa
     1381 aacccaaaaa tctctgccgc gcttagaact gtcaccggtc gaaaagtaaa acatcttcga
     1441 tcttcaggtc aaattcccgc tacaatttat ggccacaaat tcgaacctat ttcaattcaa
     1501 ttttcatctc tcgagctaaa caaaattttt gaacaagtcg gtgaatcttc cctggtcgaa
     1561 attattgttg acgaaaataa ttatccgatt atttttaaga atcctcagtt tcaccccatc
     1621 agtagtgact taattcatat tgattgttat aaagtcaatc ttaaagaaaa aattaccacc
     1681 actgttcctg tcgaacttat tggtgaatct ctcgctgtta aaaatggaaa tactctcatg
     1741 caaattctta gcgaaattga aattgaagcc cttcccgttg atttaccaga taaaattgaa
     1801 gttgatattt cttcactcat tgaagttgat aataaaatta ctgtggctga actaaacgtt
     1861 gatcgtgaca aaattgaggt caaaaacgat tctgagcaag cagtagtgat tatcgaagca
     1921 cctcgggttg aagaagaacc agtcgtcgaa gctgaggccg agttgactcc tgaagatgtt
     1981 cctgctaccg cccaaaagac ggaagaagaa aaagcgacta aggctgccca ggaggcgtcg
     2041 gagaaggacg aaaagtaaag taatttttgc tgatatcttc tctgattggg tcgaggtggt
     2101 gggctgactc aatggcttca aatgcttttt cttgattctt taatcggtaa tagataacgg
     2161 caatttccat ccaatcatcc gcatttttca tttttttaat aattatttgg tttaattttc
     2221 ttttcagttc ttcttcttta ttttctttta taaaattttc gacatcttct ctttttagtt
     2281 tactttcaat ttttttcgcc tcttgccaat ctcccttgtt ggccagtaaa taccattttt
     2341 gcagattctg gtaatatctt tctccctttg aaataggcca aatcttttga gcatttatta
     2401 aaatagtcca aattaataaa ctgataaaaa atattttctt cattatcttc ccattttagc
     2461 atgtcactat tttaaaaatg ttaaaatact ccgatgttta ttctggatat ttccaaaaat
     2521 tatgattcag ttctccaaaa agctgtcgag actattttgg cgggaaactt gattgtcttt
     2581 ccctccgaca ctgtctatat cttggcagtt gatcctacta atcaaaaagc ggttgattta
     2641 cttctttctt ttaaaaaccg ctggccagga aaggctattt ccattgccgt ttctgatata
     2701 aaaatggcta aaaaatacgt taaactagat gaaatcgcta aaaatctcta ccggcaactt
     2761 ttgccggggc catttactat tgtttctccc ggtctccatc aggtagccaa tggtatcgaa
     2821 gctgagaacg gctctctcgg aatccgcatt cctcagtcaa actatatttt agatttagtt
     2881 aagctcttag gaagacctat tactgccact tccgctaatc tttcaggccg gactcctaat
     2941 tattctcttt cttcttttct taaccctctt tcccaaaaga agaaggattt aatttcttta
     3001 attgtcgatg ctggtaaatt acccagaaac aaaccctcaa ctgtcatcga cgccctgggt
     3061 agtgaaatta aaattcttcg tcgtggcgat ttaatcaccg gaccttatca aactctaatt
     3121 tctaaatcag aatctgaaac cgctaaaatc gccaagtttc tttataaaaa agcactaaat
     3181 atgggtaata aaaaagcgat tgttttttca ttatccggtg atcttggctc tggaaaaacc
     3241 gttttttcca gaagtattgg ccgggaactg ggaattaccc aaaaaatttt atcgcctact
     3301 tttgttatct ataatcagta ccctcttaat aattctttat ttaatgtttt ttatcatttt
     3361 gatctttatc gactaaacac cgaaacagaa tataaagaaa tttcatttct cagtcacttt
     3421 ttacccagta ccatttcttg tctagaatgg ccggaaaata tgggtatttc tttttatcag
     3481 cagcttaaaa aaaaggcaaa tttaattacc gtcaatttta cttatttaaa tgatcacacc
     3541 cgggaaatat ctttttcttt tcccaaataa tatgtatatc cttggcattg aaactagctg
     3601 tgacgacact tgcgctgctt tattaaaaga tcgtcaaatc ataagcaaca tcatttcttc
     3661 tcaggtcgat cttcaccggc agtggggtgg ggtcgtgcct gatattgcca aaagagccca
     3721 tcaggataga attttaccag taatagatac ggccctaaaa agggcaaaaa taaaaattac
     3781 tgacgtcaat ttgattgcgg ttaccaaggg tccgggtttg gctatcgctc tctcagttgg
     3841 aataaatacc gccaaagaat tggctatcaa atatcacaaa aaaattgtcg gtgtcaatca
     3901 cgtcgaagga catcttttgg ctaatttttt gacaaatagc cacggaagac caattcgtcc
     3961 ttttattttt ccctctctgg ctttaaccgt ttctggcggt cacacaaaaa taattcttat
     4021 caatcagata ggggattatg aagtagtcgg ggaaaccctt gacgatgctg ccggcgaagc
     4081 tctggacaaa tctgctaagc ttttgggcct cggttatccc ggtggtccgg ttattgaacg
     4141 tttagccaaa ataggggata gtacctttct aaaacttccc actcctatcg ttgataaaaa
     4201 aattctggac tattcttttt ccggtttaaa aactgccttc tattatcaaa ttaaagactg
     4261 gccaaaaaat aaaataaatt cgcatcttgc tgatctcgcc gcttcttttc aaacagcggt
     4321 ttttgatacc ttacttagaa aatataccct agctattgaa aaatatcagc caaaatgcct
     4381 tttactctct ggcggagtgg ccgctaattt agaattaaga aagagaagta gagagctagc
     4441 caaaaaatac aatctacctc tctatttacc ttttaaaaaa gaactcaaca cagacaatgc
     4501 cgctatgatt gccctggctg gttattatca gtcacaaaga ggagactttg ctaatcctcg
     4561 tactttagac attgattccc gtgctgagtt ataattgaat atgagaattc aactttatga
     4621 tcgaggtttg ccacccggca aacaattatt tgccaatctt caaactctct gtcagcgttt
     4681 tcaagtcgac tatgacccag aatacatcaa ggatatgacc cgagtttatt ctatgggttt
     4741 acagggtaat actattctct taattgatag tgaggtggtt ctcatcgata aatatcctcc
     4801 tctaaaggaa ctcgaaaata ttctttcaga atatcttaaa taatctcttt acgttttcgt
     4861 caataatttc ttcagtttcc tcaaagcttt taccccaaat ataggctatt ttttgataaa
     4921 caaatttcac ataagctggt ctattttctt gaccccgaaa aggctcaggt gtcaaaagag
     4981 gggagtcggt ttccgccaag attctgtctc tcggaatttt tttcaccact tcttccagtc
     5041 cctcctcata agttaagttt ccatcaaatc caaagtacca ctttggctcg aaggagctac
     5101 caggcgaagt cggctccaat tcgagcactt tttttatcct ttttttaccc ccactataac
     5161 aatgaaaaac cccttttaaa tttttatatt cactcagtat ttcgactact tcatctaccg
     5221 cttttcgggc atgaattatt agtggtttat tgtatttttg ggccaactct atctgtatcc
     5281 taaaatattt ttcttgcttt ttagtgttta ccttacccaa aaattccaaa ccacactctc
     5341 ctatcgcgat tacattcgga tttttagcca aagcttccaa taaatttata tcatcagcta
     5401 tctcttgggg atgtattcct actgccgggt ataaaaaaga atatttttgt gccaacaata
     5461 aattattttt agaattttct aagttcgatc ccgacaaaat cactgcgttt aaacccgctc
     5521 ctctcgatat gtcgcaaaat gttggctcta aatgagcgtg ggtatcaatc ataaaaattt
     5581 tattatttaa ttctcggaaa taaaccgctt tccaaacctt ttatttcttt gtctaattga
     5641 tccagtattt ttttagatgt ttctggcatt atcgactcta aataagtagc cgctattttt
     5701 aaattttgag cacattcagt taatatttca attcttttcg gatcatttat ttcaagtttc
     5761 catggcgtca cttcgtttag tcgattattt gatttatcga tgtaattttc aaatacccaa
     5821 ccaattgctt catcaaattt taagttattt atcaaaattt tatatttatt atcgattatt
     5881 acttcctgat tatttatttt taaaccttct cccagtttac ataccctact cactaaattt
     5941 cccagattat tagcgaggtc agcattatat ttttctttaa atctatcaat ccccacatca
     6001 gcatcattct cggtcgggaa agatctggcc actaaatacc tagtcccgtc gactccaaat
     6061 aaatcaatca gttgacttgg tgaaattaca ttaccaagtg attttgacat cttttggcca
     6121 tcaatcatat aaaaactatg gatgtaagtt tttacaggta atttaatttc agccgacaaa
     6181 agcatagctt gccaaattac cgtatgaaac caaagtattt cttttcccaa aatatgtaaa
     6241 tctgccggcc aaaactcctt tttattttct aaaaattggg tggcgctgta gtagttaatt
     6301 agagcatcaa accagacgta tacagtctga tttttatccc aaggcagggg aattccccag
     6361 ctgacatttt ctcgtgatag agaaatatca ttaattcctg cctttaattt ggctaatacc
     6421 tctgactttt tactctccgg aaaaatataa ttgcttgtat tattttcaat taatttaatt
     6481 atttgaggaa tatatttcga taatttaaaa aaccaatttt cttcagattt tttgactgtt
     6541 ttctctgctg agtggagtgg acaatgtccg tcaaccaaat cagtttcggt taaaaatttt
     6601 tcacagccaa tacaatacca tccctcatat tttgccttat aaacatcccc tttatcatat
     6661 attttttgaa gtagttcccc cactaccttt tcatgtcttt ggtctgtggt tctaataaaa
     6721 aagtcataac taatatttaa aactttccaa gtttcagaaa aacggggtga aattttatcg
     6781 caataatctt ttggacttaa tccctctttt tcagctactt cagccacttt ctgtccgtgc
     6841 tcatcagttc cggttaagaa aaaaacttct tctcctgtct ttcggtgaag tcgcgcaata
     6901 atatcagcgg ctaccgtggt acaggtatga ccgatgtggg gaatatcgtt aatataataa
     6961 atcggagtgg taagataaaa cttcttcatt accctattct accttattgc ttgattactt
     7021 tccttttagc tttaactttt ttcttgacca cttttttttc aacctctttg tcaacttttt
     7081 tagaatatct tgttttgagc cttgaagctt tattttttga gaataaatgt cttttttcgg
     7141 ctttatccaa aactgcttgt accttggcaa aatttttctt gtctggttta gctagaaata
     7201 ttttgacgat ttcttttaat ttatttttga aaacctggtt aagtttttta tttttaaccg
     7261 actttctgag tgattttttt gctgatcttc caattggcat aagtcgtatt ttatcctatc
     7321 gccaatctat ttacaataat ttacaatagg actatgtcac ttcgtctccg aattcgctcc
     7381 tttactcaac agcgtcaaaa gagtatattt tcagcgactg tcgttttggc cattactttt
     7441 gctttctctg ccattcttgg ttttttaaga acccgttttc tctattcaat tttttttaaa
     7501 tgctgtcttc ttgagcttga tgcctacaac gcggctttcc gaattcctga tttaatattt
     7561 aaactactag ttactggagc cctttcagcc tcctttattc ctgtttttag tagttatttg
     7621 cataaagata aacaagaagc ttatcgcatg gcttcttctg tcattaattt actattaatc
     7681 tttttttcag tcatctcact tattgcctta atctttgccc accctctttc ccagttaatt
     7741 gctcggggtt tttctcctta ccaaatcaac ttaatgtcga atttaagccg aattttactt
     7801 attgctcaaa tattttttct tatcagtaat tttttaaccg ccattcttca ggtcaatgag
     7861 acatttatta tttctgcctt gtcaccaatt atttacaatc tctttataat ttttagtatt
     7921 tatgctttag ccccaacttt tggcatttat ggggttgttt ggggggtggt cattggcgcc
     7981 tttttccata tggctatcca aattccttca attaagaggc ttggttttgt ttattataaa
     8041 attgctagtt ttaagttcag tggtgtcaag gaaattattc gtttaatgat tccaagaact
     8101 ttatctcttg gtctttcgga aatagaaagc acggtcactt tattttttgc ctcaagtcta
     8161 agcgccggtt ccatttctct cttaaattta gccctccagt tgatgtatct tcctagtcgt
     8221 atttttggta ctaccgtcgg tcaggcatct ttacccgtcc tttctaaaaa tattgccaaa
     8281 aatcaattga atctttttcg ggaaaccgtc agaaagacga ttcttcagag tctttttatc
     8341 gcttttccca tcggtgtatt aattttaatt aatcgtttgc ctattgttcg cctagccttt
     8401 ggaagcaaac agtttccttg gtcggccact ttacttacag ccagaacttt ggcttttttg
     8461 actccggcaa ttgtctgcca agccgtgatt caaattttaa ttcgttcttt ttatgctctt
     8521 catgacacca gaaccccttt aaaaatttcc ggaatttctc tgtttttcaa tattgttacc
     8581 agttattttt ttattaagtt cacctctctg ggtatagtcg gtttagctat cagcgcctca
     8641 attgcgaata tcatccaatg tttaggtctt tttgctttat ttatctggcg ggttgatggt
     8701 caaggctggg aaaaaatgtt tttttccttt agccgtatcg ttttaagctc gatattatgt
     8761 ggtttaacct catggcttac ccttcgtttt ttggatcttt tcgttttaga caccagtaaa
     8821 accctaaatg ttttgttaat ctttggtctc tcgattgttt ctggcctctc ttccttctta
     8881 atttttacct ggctttttcg ttctcctgag tttcttcgct tcaagtcata ttttcagcgt
     8941 ttctttcatt ttctttctta gacatttttg tttatactgt ttatagtatg cctgctaaaa
     9001 aggagatttc tttattacca aaagaagaaa acctaaacac tttttccgcg cgctttcttc
     9061 ggtgggtgac gagcgttggt cgagtggtaa taatttttgt cgagctgata gtgattggtg
     9121 cttttttatc tcgtttttgg cttgatcgaa aaaactcaga tctgtcagaa acccttcgcc
     9181 agcaaaaagc tattttgggc agcactcaag aatttgagaa agactatgta tctctccagg
     9241 agcgtttaaa gtttataaaa aatttctaca ctaccgagcc aaaatatatt gaatcactta
     9301 attccttaat tcaaagtatt cccgagggaa tttattttga aaacattagt tttaatagcg
     9361 gtgaaaagtc gagtcctatc actggtagtg ttgaatcata ttcctatcaa gaaaatgcca
     9421 tcattgattt tattgttaac ctcaaactta atcctgatat cagttcggtt aatgttcaaa
     9481 aaattgagaa aaaaaccaag gacagtaaat tttatttaag tctttcttta accttcaaaa
     9541 aaccatgaac cccccagttt ccaataatct aaatatctat tctaaagtta accctctact
     9601 ttcctatcta tctaaacagc gggaaaacaa taaatttatc aaatctgttg aaattagtgc
     9661 cactttttta ctaatttctt tttttacttt ttttgcaatt aaaccaacct tattaactat
     9721 ttccaaactt gttggtgaca tcaaagctaa agaaatttta gtcaaagaat taaaagcaaa
     9781 aattaataat gtgattattt ctcaggattt attttcacaa gttcaggaaa aatatgccct
     9841 ggttgaagct agcctaccaa gcagcccccg tttttatcag gccacttctc aaattgttgg
     9901 tctaactgaa aaaaatcagg tttatttaga taaaataaat tattcaatct cagattctaa
     9961 tttttattct actagtataa gcacttcttc ttcttttttg tcagccactt ctcttctttc
    10021 gggccttctt caaaacagac gactttttca aattgatcaa atttcatttt ctcttgaaaa
    10081 agacacgggt agtaaaacag ttaatataaa cctaccttta aagatttact attggccaaa
    10141 aaatgtccaa aattaaattt tctaaatacc tagttttaat tagtattttt acttttattg
    10201 ccattttttt ctcagttgtc tcccaaagtt acaataattt aataaaatca acagcagaaa
    10261 ttgaaaacaa tcccttaatt cgttcagttt ctcccgatct tgatctcaat gttctcaatg
    10321 agattgaaaa aagacaagaa ttatcatcac ctagtttaga tgtcaacaac ctcaatgaag
    10381 ttgaaaataa aattgagcct gttttatgaa agataaaaaa tttccgacaa tattaggaat
    10441 tattttcctc cttgtcatcc tatttctagg agtttttctt tccaccaaga caaccagttt
    10501 aaactcaaaa gcgagtgtct cttgtgaacc agtcaatcct caaattacca accttacctt
    10561 tggttctttc gatttttctt ttaccacccc ttctccttgc agtgcgactt taagtataaa
    10621 taatattatt tatcaggacg cttccctttc cgaaacaact cattatttca agatttctaa
    10681 tctttcccct caaaccagtt ataaatttag tctcatttct ggttccagta cttattctcg
    10741 ccctgagtac gtcctaacta ccgcttcaaa accaaattcg ttgactccct cctcaaacct
    10801 ggcttgggga agggttatcg attcaatggg caatccaatt tcgggagcta tcatttatct
    10861 tactttacct ggtagtcaag ctctttccgc cttttccaat aaaaatggca attggaacat
    10921 ttctttagct gccagcttta atgagcaaaa aactgactgg tttactcctt cgactactgc
    10981 tgaagaagat attactgttt attctccaga tggacaggtg acgcaattga ttaacccatc
    11041 cagcaataac gaccctgtgc cggatattat tatcggtcaa aattactttt ccaatgtccc
    11101 taccccaatt tcttctacca aaagcagttt tgacatcaac aacccagaac tgagttttgt
    11161 tagccttagt attgctaatc caagagaagg agaaaacgtt tccactctaa aacctgatat
    11221 ttttggacaa ggtccagctg gtaaaaatat tcaattgaat ctagatggta aactgagtag
    11281 tattactgtc gctgacaaca attcttggaa tttttctcct tcccaaaatt tatcaattgg
    11341 atctcaccag ataaccttaa cttatcaaga ccaaatagtt accagaaatt ttttggtaac
    11401 cggtaccagc gattacttat cctttagcgc tactccttcg gccaccttga ttcccactca
    11461 aaacattttg ccaaccattg tttcttcgcc tacccttacc actatgccca cttcagttcc
    11521 tactattaga accgctaagc cttcaactac ttctaccctc tatgaaagcg gtaactctct
    11581 tccaacttat ttaatatcga ttttatctat tttatttttt ggtgtatctt tttattactc
    11641 tcattgatga aaaaatacgg tattttaagt cttctatttc tacttttttc actagccgga
    11701 gttctctatt tagtaaaaaa taatcaagac ataatagaca gagccgcagg tgaatataac
    11761 ggcggaagct tgtgtaacgg gtacatcaat aattggtgtt ctcaaaacgg gatcactgct
    11821 actttatcaa aatgtcccgg agagtggaat ccgtcgtgtt ggaaaaatac tgttgttgtt
    11881 agagatggat catctctctg tttagaaaga aattattgtg gtagtcaaca actagatgtt
    11941 tattataatg atggaggttc ttgttgggtt gattattggg attattcttg tgatgcgccc
    12001 accgctactc ccatacctcc aactaatact cctacccnna aaccaaccaa tactccaacg
    12061 ccaactaaca ctcccaaacc caccgccacc ggaacccctg agcctactgc tacgaatacg
    12121 cccattcctc caacaaatac tccaattccc tcccaagtta ttactgaggg tcccagtcca
    12181 actcggataa ttttactcga atcaggtgtc gaatttcctt cgcaaattct cacccttgtc
    12241 ggtggcatca tcaccttgct cggatttttg atattattgt agtgtgttag ccatcattga
    12301 agctcttcgt cc
//
LOCUS       DHGB01000002           20127 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_11563, whole genome shotgun sequence.
ACCESSION   DHGB01000002 DHGB01000000
VERSION     DHGB01000002.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 20127)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20127)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20127
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_11563"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    2824..2938
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3444..3500
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18526..18623
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5906 a         4671 c         2509 g         6763 t
ORIGIN      
        1 caatgaaaaa accaaaaata tgaagagttc tttaattccc tcaatcatta ctatctatga
       61 agatcgttct tttgatttca ttatcaaaaa acctccagtt actgacatga ttaaaaaaat
      121 cactggcatt accaagggta gcggcctaac tggtcgcgaa aaagttggaa aaatatcagc
      181 tgcccaaatt gaaaaaatcg ccaccgacaa aatggaagat ctaaacacta aagatttaga
      241 agctgccaag cgcatggtcg ccggcaccgc ccgctccatg ggttttgaaa tcacagacta
      301 atcatcaatt atcaatcttc attcttcaat aattgaagtt tgaagattga acatttaata
      361 atttaaaaaa aatgagcaaa acctctcaag aactaaaagc caaaaaggcc aaaaaagaac
      421 ttgcggtgga agatctaagc aaagtcaaag atcgcaaggc ttcagaaaca gttaccgaaa
      481 ctaatcctga agtaactccc aaaaatgtta aagaagtcgc ttcaaccgac gaagctacaa
      541 aggtagcaaa ggcggttcgt cttcgcggta aaaaatattt atcagccaaa aagaaaattg
      601 atgttcttaa gttttacccc ctaaaagagg caattaaatt agtcaaagaa acttcttttg
      661 ccaaatttga aggtaaaata gaagctcatc taaccgttat cgacactggc aatcttgggg
      721 aaatttcttt tccttattta gaagttggca ctaagagaat ccagctgcta gatgataaaa
      781 tattggccga acttaaggac ggaaaaataa atttcgatat cctaatcgct actaccacta
      841 ctatgccaaa acttttaccc tttgcccgta ttctcggtcc caagggtctt atgcctaacc
      901 ctaaaaatgg tactttaacc gataagccag aagaagccat gaaaaaatta gccgtggcta
      961 aaactattat taaaactgag agtaaagccc cagttgttca tttggttgtg ggtaaggtct
     1021 ctcagcctga aaaagaatta gaagccaata tcaacgagct tattaaagtt atcaaagctc
     1081 ctaagatcaa aaaactagtt ctttctgcta ccatgggccc aggagtcaag gtactgattg
     1141 aaaaataaac ctattattta aaagctaaaa tccaccattt aggcgggttt ttgttaaact
     1201 atcttagtgc ccaaatattt atccctcctt tttctctact accttcgttt ttttgcccgc
     1261 cttcaattaa aaaaagttaa actcctcaac cccaaactga ttaccgttgg cattactgga
     1321 tcagccggta aaacttccac tcttttggca actgaggcag ctttaaaacc aaactttaga
     1381 gttaagacca actatggcgg caattctgag tctggtatcc ccctcaatat tctcggtttt
     1441 aaaaatcccg attttacaat tgtttcatgg cttaaaatcg ctcttctcac ccctttcaag
     1501 ttactaacaa actggaaaat ttatgacatc tatctcatcg agatgggcat tgactcccct
     1561 actgccccta aaaatatgtc ttatctactc tctatcgtga aacctgatat tggcgttttt
     1621 ctcaatgtca cccctgtcca tttagaaaac ttctcttctc ttgatcaaat agccgaggag
     1681 aaggctaaat taattagcag tgttaaaacc gccatcatta ataaatctga ccctttagtt
     1741 aaaaactata gcaaaaataa aaatactatt gccattaagc ccattaaaat tgatattccg
     1801 ggttttattc ttcctcatat ctatgatatt tctcttggcg ctgctctggc cattgctaat
     1861 gctcttaaga ttgacagctc tactgccatt aataatttaa aaaataattt tcatctccca
     1921 cccagtcgtt catcaatctt taagggccta aataattcca ccatcatcga ttcttcttat
     1981 aattcttccc ccttggccac ttccgaaatg ttaagctttt tagcttctta cccctctccc
     2041 aaaatagctg ttctcggcga catgcgtgaa ctgggtgccg cctcaaagtt tgctcatcaa
     2101 aatctttata aaaccgtgct taattttacc gatcaagtaa ttagcgttgg tcctctaacc
     2161 agtcgctttt ttggctccaa agcccaaaaa tttatgtact ggtggcaagc agctgattat
     2221 ttaaaaaata atctacctca aaatgccact attttagtca agggttctca aaatactatt
     2281 tttctcgaag aactagttaa agaattaatt ccatcaggtc aactaaaaca ctacttatct
     2341 aataatcaaa tctgtcgcca atcaaaatac tggctaaaaa caaaagctat ttttaaatct
     2401 caaaactctt aattgaaaat tgtagattga agattaaata ttttatccct taaattcttt
     2461 ctctttttta atttcttttt caattaaatt tctcaaaaaa actgacctgg gaatcattct
     2521 tttttgagct acccagtcaa gatatgccaa aattttggga ctaacaaaaa aattaaaccg
     2581 aacatctatt tctttctttg cctcttcaat ggcccgatct aacaaatctt ctatattttg
     2641 tttatcgtat tccatcaatt ttaactttgg gctattaatt ccttttaaaa aaagtatttt
     2701 tgttcccttt ttataaaaaa ctaccaccgg tttacacaga tcgagagatt ttgaaattaa
     2761 ataccccatt gtggtactgt gtcccgagat ctccatcatc accacatcag ctttttttac
     2821 ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     2941 taaaaactta agcgactata caccacttta atcaaaattt ctacctattt gttttgttta
     3001 gtcttccgac tttttaagtc ctcaataaaa tttatcaaag ctacatctac cttttttcca
     3061 ttttcagtcc cccagtactc atctttttgt gacaatattt catccaaact tatcctttgg
     3121 tgaatcaatt tacttgctcc gtaaagcgat cttctcccta aataatcaac ccatttatta
     3181 ggcatagaac taacaatatc tttaccagaa agacaaacaa tctccaatac agttcttaga
     3241 ttaggatttc cagctgaata cttattaatc gtctcttgaa ctatttcttt gtccacttac
     3301 acctccttta ttattccaat tctctaccac agaaaaagta acattcttct gtaatcaaga
     3361 attttaataa tcaaatttac aacttcggga ttttagcaca gttatccccc tttgccaacc
     3421 accaaaacta gtgtataatc cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn atatgccaaa aatacaaaaa atagaaaaag ttgaccaggt
     3541 tgtcgctaaa ctctccgaat ccaaatcagc cgctttaatt caatatcagg gtcttagtgc
     3601 tggtgatatt agcaatctcc gcgccaatat taaaaaggcc ggcggttcaa tggaagttat
     3661 caaaaactcc cttatttccc gggctctaaa caaaattggc ctgtctttac ctgaaacatt
     3721 aactggtccg accgccattg cttattgcga cactgatgaa atcgcccccc ttaaagaaat
     3781 cgatgtcgtt aataaaaata aggaaaaaac ctttttcaaa tatggcattt acaataataa
     3841 attactctca ctggaagaat taaaaaaatt catctcttta ccttcaaaat cagcccttgt
     3901 tgcccagttt attggtggac ttaaaaatcc tcttcagcgc ctcgtctatg ccatgcgttt
     3961 caatcagcaa caactggttt taaccctaaa agccttatct gataaaaagg ctacagaaac
     4021 aaattaatta ttatttattt tatgccccac cggcgnnatt ttaactttat tatttattta
     4081 ttaattattt aaaaataaaa aaatggataa aaaattagat actatcctta ctcaaatttc
     4141 tgaactttcg gtactcgaac tctccgaatt ggtcaaagct ctcgaagaaa agttcgatgt
     4201 taaagctgtc gctatgtctg ccgcttcgcc cgccggagct tcagcgcagg agggtcctac
     4261 tgcccccgtt gaggaaaaaa gtgaattcga tgtcaccgtc accaatgccg gtaccaacaa
     4321 gatagctgtt atcaaagcta ttcgcgaatt caaacccgag ctcggactta aagaagccaa
     4381 ggatcttgtt gatgcctgtc ccgccgatct tggaaccatg aaaaaagaag ctgccaccga
     4441 agccaaaact aaactcgaag ccgctggcgc tactgtcgaa ttaaaataat tattttattt
     4501 taaacctaaa atccccctga gtttatcgaa gggggatttt tgatatttaa tttccagttt
     4561 cagatttata tttttgcatt accattctta taaattctac aaagatcggg tgcgggtcaa
     4621 ggggtcggga acgatactct ggatgaaatt gagttccgat aaaaaatgga tgacctttta
     4681 attcaattgc ctcgaccaac tttccatccg ggcttgttcc actcaccact agtcccacct
     4741 tttctaactg tgctctaaat tcattattaa attcataacg gtgcctgtgt ctttctttta
     4801 ctaaattttg gccataaata ttttgaagta atgtcccctt attaattttg caaggccatg
     4861 aacctaatcg aatagttcct ccatactgat gttttttcaa atatttttcc tgttccggca
     4921 tcaaataaat tacaggaaat ttggtcttcg gatttacttc tattgaatta gctccatcta
     4981 atttagccac attacgagca atttctacca ctgccatttg catcccaaaa caaagtccca
     5041 aaaatggaat cttattttcc cgggcaaact ttatcgcttt gataattccc tcaactccgc
     5101 gactccccca tccttgaggt gctattatcc catcacaatt ttttaaatcc ccaatcttat
     5161 aattatttga atcgatcgcc tctattttta gtcttactcc gttggtaata gctccatgtt
     5221 tcaacgcttc tatcacagaa acataaacat ccttatgatt tccttttcca gatttaacat
     5281 atttacccac caaagcgatt cttatcacct tttttgcttt atccattttc tttaccatct
     5341 ctttccagtt ttttaattct gttggacatt ttcgaagctt aaaatgcttt ctaattttct
     5401 tcattaaacc ttgtttttcc aaaattaagg gcactttata aatagactca caatctgtat
     5461 catcaaaaat tgactcatca tttaaaaagc atctctttgc caaagtttct cgacgaggca
     5521 aatctaaacc tgtctcactt cttgtaatta ccaaatcagg aactattccc actgatctca
     5581 attctgaaat cgcatgttgg gttggtttag tttttaattc accaacattt cttaaaaatg
     5641 ggacataaga caccatcacg tacattacct ctcgttctct tttaacatct ctggcagcat
     5701 acaaaaatgg taaattttca atatcacctg tcgttcctcc tacctcaatt actacaaaat
     5761 catatccctt ttccaattca taaaatcttc tttttacctc gtttggtaca tccggaatca
     5821 tctctacatc tcgtccttca tacttaaaag ctctctcatc ttcaataatt tttttataaa
     5881 cttttcccga agtaatattg ttttctttgg aaaaatcctg atttaaaaat cgttcatagt
     5941 ttcccaaatc ctgatcgatt tcacccccat cagccgttac aaaagtttct ccgtgttctg
     6001 ccggtctcat tgttccagca tcaatattaa aatacggatc aattttaata tgatttactt
     6061 taaaaccaga tgattttaac aataggccta tagatgctga tacaattcct tttcccaaac
     6121 ccgataccac tccaccaact acaaaaatat acctcattct cccattttat acttatgcta
     6181 cctcaattca atgtctatct ataaaaatag tctataataa acttaatttt tgccgccttt
     6241 taaataaata tttcattggg tttttttcta tataccgttt aactcttaaa tattctactt
     6301 tatctctaat aattctttca taataattcc tttgccataa tctcttaaaa aatcttggcc
     6361 aattttgatt tttaacattt tggatatatt cccaaacaaa tattgactta aatactccaa
     6421 taatattacc cagattattt cgggcgggca taaaacccgc ccccacattt gaatttaatg
     6481 tacgggcgac tcttgtggtc gccctattta ttcttaaaat cccatggata tgatctggca
     6541 taattacata ttcatctaat ttaatattaa atctcatctc caaatccgac caacactttt
     6601 ctgctaacat tcccatctca ttcaattcaa atttatttat attcttccaa aacaaatttt
     6661 tcttgctctc taaatcaatg gtgacaaaat ataaaccatt cctggaataa tcataccctt
     6721 caaatctaat cgatctcctt ttgccatata tctgtaatta ttattacaga ttatttacaa
     6781 caattccact ttattttctg ccttcagctt atcgactact tctcccactt ttcccgtcat
     6841 ctctttttta caaatcaaaa gggcatcatc tgtttcaatt accaccaaat catctactcc
     6901 aattaatgct gtatattttt tcgatcttac ataattattt ttcgcttcaa tttccacccc
     6961 tttcaattga taatcgacga ttaaagattg ataattaact acgctttccc aggttccaaa
     7021 atcaatccat tcaaaaggca actcagtcac caccgcttct ccttttttat aaagctcctt
     7081 cgttacctcc tcaattgccc cctttggcat cttggcatat tcagtcacaa tctctcctcc
     7141 accgacaata ttcatcagtg gctcatacca atccatccga tattttttat atatttccat
     7201 caaattattt ggtgtcattg ttgtatgatt agcatgaatt aacaacttat ctgaacccaa
     7261 atactgttta attttatctt cttcgctcct atcaatataa tcagccaccc gataaacttt
     7321 tattccattt tcttctttga ttaggtcacc cttgaccaga taatcaaccc ctcgaacaat
     7381 ttctctaggg gctatccccc ctgtcaaata ttccccattc gactcagcga ggtcccccat
     7441 cacctccatc atttctataa atttttcttc cggttttctc ataacgtcag cctgaaccaa
     7501 cataaaagct tcgtcaccaa aacccttttt aattaaagag gccgctgcaa acccagtcgc
     7561 cggaccttga tttctcatct ccggctcaat aaaaacattt tctggtaaaa tttccggaac
     7621 ctgcttttta gcaatctccg cctgagccgc attagtctgt aaaaatattt cctctggaga
     7681 aaattttagt cttagcgcct cccagtttaa ttgaaaaagc gactcttctc cgatgaggcg
     7741 taaaaactgt ttaggtgaac tactgcggct ctccggccac atttttgtac caactccacc
     7801 acaaatgatg actgctttca tatttatttt ttttgacaat aatttgtaat attttgaatg
     7861 gttgttttaa caattctctc aattgcctct tcgctattgg aagcgttgtg aggggtaaac
     7921 acaacgtcat cccgatcccg aagcatgtga tagctcagta catcctgtaa atcattcctt
     7981 gttggtcttt gactcatgat cattgatata ctctctagct tatcttcttc ttcaaccaca
     8041 tctagtcctg ctcctctcag aattttttta tttagggccc agatcaccgc actgctttca
     8101 acaaccgccc ccctggaagt gtttacaagt atgcttccct ctttcattat tttaatattt
     8161 tcacgattga tcaaatgata agtttccttg gtagccggca catgtaaagt cactacatct
     8221 gattctttta acagagtttc taaatttacc atttttattt tatatttctt cactatttta
     8281 atctccggtt ttatatccac tgatataatt ttcatcccaa aaccatatgc aattttagcc
     8341 acattactgc ctatttgccc gaggcctatt attccgattg ttttaccaaa taaatcaact
     8401 ccagttaacc cctcgggact aaaatcgtca tcctctaccg cctgatgcgc ttccacaatt
     8461 tttttggcaa ctgccaggat taatccaaaa gcatattcag ctaccgtatt tgatccgtag
     8521 attggcacat tggtcaccag tatttttctt tttttgcatt cagccaaatc aatatggtca
     8581 actccggtcg acctcgtagc aatcatcctt aagtcgggta atttatctaa aatttcttta
     8641 ttaattactg aataaattcg tagtgataaa atatcatatt ttttggccaa ttccatatta
     8701 tcttgaacat taccttcaaa tattcccact ccaaaagcat ctaaattaat aatttctcgt
     8761 tcaattattt ctctctccca acttttaact ccaaaaaaag caattttgtt acccaccatt
     8821 gacctatttt acaacatttt tttaaatctc attttttgat atcttgacct actagtatcc
     8881 tctgtgtttt aatgaatata attaaagatt aatttgatac aagaggagac accaaatgga
     8941 ggaccaaacc atatcacaag aaaaacctgt agctcaaaaa tccaccccaa aaaaagttaa
     9001 tggcatcagt attgacaatc tacccgatct tttgactgtc gctgaagtcg ctgatttact
     9061 gcgtgtttct aaactcacca ttaagcgctg ggggaaaaga ggtaaactcc cggctatccg
     9121 tattaatgct cgtggtgatc gtcgctaccg taaagaagct gttctttggc tactcggtat
     9181 tcagtccgaa tgattttctg tagagacgca ttatgatgcg tctctacatt ttgatttgcc
     9241 ttaagggcgg ttggattaat ttccaactgc cttttttggt tatactatca ctcgtcctcg
     9301 tggtgtcctc ttaaccagtc cctttttcat taaaaatggt tcataaacat cagtaatcgt
     9361 ctccacgtct tcggtcagcg ccgccgcaat attctcgact cctaccggtc ccccaccata
     9421 ctccctttta actacctcca aatacttcct atctgcatca gataaaccca gttcatctac
     9481 cccgagtttt tctagagcta tctttgcctc agttatcgtt atcaaaccat cattattaat
     9541 ctgagcataa tctctcactc gtttaagcaa tctattggca attctaggcg ttcctctcga
     9601 ccttttggca atttcctccg ctgcttcttt ctcaatttta acctttagat actccgccgt
     9661 tctcataata atttccgata aatttttatc ttcataaaaa tctatctgct gcacgattcc
     9721 aaagcggtct ctcatcggtc cggaaattaa accaattcta gtagttgccc caactatcgt
     9781 aaatttctgt aattttagtc taaccgatct ggctgatggt ccctttccca aaactatatc
     9841 caaaacataa tcctccattg cactgtataa agtttcttcc acaattttat tcagacggtg
     9901 aatttcatcg ataaaaagca aatcgccttt tttaagagag gttaaaattg aagccaaatc
     9961 tccagctctg gttaaagccg gtccggaagt aatttttata tttgtcccca tttccgccgc
    10021 cagtagcatc gccagggttg tttttccaag gcccggtgga ccataaaaca aaatatggtc
    10081 aagagcctct cctctttctc tagccgcttc aataaacact tccagctgat cctttagtct
    10141 tctttgtcca ataaactccc ctagtttttt tggtcgcagc gacagctcaa tttccttatc
    10201 ttccttgccc gccgtagcat ctgtgaagga gggaattttt aattttggat ctaaattttt
    10261 atccgtcatt tatctaccta aattttgcaa acaccaacta accttatctt ctaatatatc
    10321 tatatctttt ggcatttttt taatcacgtt ttcaatttct tttctttcaa atcctaactg
    10381 tctcaggctc aaaaccacat catcctcaaa tagtggcaaa tcctctttta aatttaattc
    10441 tcccagtccc cctatttttg attttaaatc aacaataatt ctctgggctg tctttttgcc
    10501 tactcccttt attttttcaa aaaatgaggt atctgcctct cctattgcct taattacttc
    10561 tatcccccgg cactgctcca aaatctgggc cgccgatttg ggtccaactc cagagacagt
    10621 aattagcatt ttaaataaat cgagatcttc aaaattttca aaaccataaa gagccagatc
    10681 attttccgat accgccaaaa aagtatagat cttaatttct tgtccttcac tatagttttt
    10741 cctcccaacc catatcaaat aacccactcc acctacctca acttcaatat aatttcccga
    10801 aattcggtta actcttcctt ttaagctgga aatcataact catcttaaca cagctcaact
    10861 ccgaacaaaa gccgaagtaa acccatgtgt cagcgctgcc gctactgcgt ccgaagcatg
    10921 accgggtgat attggtttct caagattcaa acaatccctt accatcaatt ccacctgctc
    10981 cttttccgcc ctcccatagc ctaccaaagc tatttttatt tggagtggtg tatattcaaa
    11041 aactggcttt ttcaaatttt ttgccgccac cttaataacc ccaatcgcct cggctacctt
    11101 taaagcactc ttggcatttt tagcaaaaaa cattgactcc acggccacct ctcccacttt
    11161 atattttata ataatttttt gaatttcctc aaaaatcttc tccaatctct cttcactttt
    11221 atccttttta tcggttaaaa ttaccccaca atcaattaac tgatagctgt taattgaaga
    11281 ttgaagattg attatccccc accctgtatt ctccagtccc ggatcaattc ccagtatcat
    11341 cgctttcctc cactactttt ctttcaattt tatcttttgt aatcatcaac tcagtataac
    11401 gggaactttg aagattatcg agccgagaga tagccccaac gatatcggtt ctaatcccct
    11461 ccagctcgta tgaagtactg taatgcaaat ccgccgcnna ccgctacttt ttcatttccc
    11521 tcccccatta tttgagccga aaataaatta tttaaagctt tatgcatcac cgccagacat
    11581 attttttcct gacaatcacc cagtactccc tcaatccatc ttttttgttc ctcacccagg
    11641 ccatgccatg aagcattgtt tatcaaaata aattttacct ttcctatttc aataatctga
    11701 taatcttttc caaaaatttc cttaaaatta gttaaatttt tctctctgtc tcctgggata
    11761 actacatatt ttaattccga cctatctaga atatttttca tcttaacaaa atcttctttt
    11821 ccttcattat caattaaatc gccagcaatt attactagtt cgctgcctct attttttgcc
    11881 tttaaaagca ttttttctaa ttgttcacca tcactatgaa tatccgccat aatcgctacg
    11941 tttagtttcc tctcattctc tgtgggaagt atcaaaagat tatcttcact tatcctttct
    12001 ttttttttat taaaaagacc tccaatcgca tatcccagaa ttactatcat aattattaat
    12061 cccaatattt ttaccgttct cattacttta ttttacccta tttcatatta tagggtgaaa
    12121 attctttttc ccacttatcc acacattcat aatatataat tttattacaa gatatatatc
    12181 aatattttct ctcattaata tataattagc tatggaaata atgaaaaacc atcccctctc
    12241 cgccctgacc accgtaaaaa ttggtggtcc ggccgatact ctaatcatta cttcctccac
    12301 cgcagaattt attgaagccc taagtcagag cgatccaaaa cggccgccga ccattctcgg
    12361 taatggctct aatattctta tttccgatgc cggtatccgg ggcactgtta tcaaaaatat
    12421 ttcaagtaat atcgagtatt taccgaataa taaggtaaaa gttgattcag gcactcagtt
    12481 aaatatcctt attcaggaca ccatcgccca ctatctttct gatcttgaag agtttgccta
    12541 catcccttca actattggtg gcgctatcta tggcaacatt cacggcttta ataaaagcaa
    12601 ctttaataaa attcttctag aaattgatgt ttttgatctc tctgaaaaga aagttattac
    12661 ccctataatt caggcctctc agttaaattg ggattatgat ttttcccagt ttcagttgca
    12721 cccaaattgg gtaatccttt ccgctaccct tcagctctca cctggcgaca gtaaactctc
    12781 caaaaagcta gtttcggata tcatcgccca caaatctcaa actcagtctt taaactcact
    12841 tggctcagtc tttaaaaatc cccctgggga ttcggccgga cgcattatcg atcaagaact
    12901 ggagttaaaa ggttttcaaa tcggcaacgc tcaaattagc ctacttcacg ctaactttat
    12961 cctaaattta ggcaatgcca ccgcctctga ttacaaagct gtcattgata ccgttcagga
    13021 aagagcaaaa agtaagttaa atttagttct tgaacctgaa attaaatttt taggcgactt
    13081 cagttaatac catccaaata aatttaggca acactagttg tcttttccat ctttttggtt
    13141 ctttaaatag gctataaagc cactcaagac ccatttttct tatccaaatc ggtgctcttt
    13201 tcaattttcc actgtaataa tcaaaacttc tccccactcc catcacgact cccactttta
    13261 atttatctaa attaacccta atccactcct cttgtctttt cattccataa gccacaaaca
    13321 aataatcggg ttcaaattta tttatttcac tttttacttt ctcattatcg acctccggtt
    13381 ctccagggga aaaccttgtc tcaagtttct catttattga cataaaataa tcagccgttc
    13441 tctttgccct gtccccccat cctcctaaaa aataaacttt gctttcactg ttttttttac
    13501 atatttctgc catcaaatca gagccagaaa ttacttcttg gcgtcttttt ccaccaagaa
    13561 tctctcctcc taccttaaac gcttgccacc atctcctcaa tcctttctga tattttaata
    13621 cattataggc ccaaattaaa ccgattccat ctatcacttt tatatcactt ttattgataa
    13681 tatccgcaaa agcatgatct tttttcatct ccataataaa ctcaggatta actgtcgtaa
    13741 tccaaatatt ctgctctttt tccccaagtc tgcgtgttaa atcatttagc aattgactta
    13801 aattactgcc aaaaaagctt aaaccaaaca tttctcgtgt ctctccagta tatttaatac
    13861 tcttggctcg ggtttcactt tgccaatttt gcctgttttt cttcatatat taacttataa
    13921 taaatgattt acttaaataa taatagaccc ctctcaaaaa atacacctaa attacttata
    13981 ttatatatct tatataatta caaataaact tatcaaattt taatatatca cgtctttaca
    14041 cttaaaatac tataagtttt attactatgt tttaatttat ttaaaaaatt aatatattga
    14101 acaatatttt tactcatata tactaccata aaacagttat aaatttaatt ttcattgttt
    14161 ttctttgtta tttatcaact caactcttat aatgcctcta atttttttag attttaggat
    14221 ttagatttta gacttttata cattatagga ttttaacttt ccaatacatc accgagtaag
    14281 ctttcgatat atttctaaat tttccatggc catccccgcc cccttaacca cacaattttc
    14341 tggttccaaa gcaaggtgca ccgccacccc aatttcccgg gtcaacaata ggggtaaatt
    14401 tcttagtttc gacgctgctc cgattaatac tatccctcta tcagcaatat ctgccaccaa
    14461 ttctgggggt gttgtttgca gggtttcctt tattactaaa attacctcat caagcattgg
    14521 tctgattact tcaaagatct catcagaatt taactctatg ttttttggca gcccataaac
    14581 taaatcccga ccgccaatcg acaacatttc tgtcttcttt aattttactg ctgtcccaag
    14641 tttaattttt actagctctg tcgtctgatc acctatcgcc atactatact ttttcttcaa
    14701 atattcagct aaagcctcat caattctgct accaccgccc cgatagcact tgctggtaac
    14761 tactccccca gaggcaatga cagctgcttc aactactcct ccccccatat tcactaccat
    14821 atttccaaat gcttctgcta ttggaatttt tgtccctatg gctgcagcta gaggtgtatc
    14881 tatcaaatta acctttcttg ctccagcttc agctactgca tcaaggaccg ccctttgttc
    14941 gacctgagtc agaccagaag ccaccgtaac caccacctct ggacccaaaa accaaaaaga
    15001 ccccataact tcccttaaaa accatttaag cattcccacc gccacctcat agtcaataac
    15061 agccccctca ctgatcggtt ttacaatttc aatataatcg ggtgttttac cttccatttt
    15121 tttggcctca tttcccgccg ctagtacttt tcgattttcc gcctcaaccg ccactatcga
    15181 cggttcgttt aaaataattc cttcattttc actccacatg atacagtttg aagtcccgag
    15241 gtctattgct aactttctta tccaggtcat aattttatat aatacactaa tgaaatcaaa
    15301 ttctaaccaa aaaagtcttt taatcattct ttctgtcgct ctaattgttg tcagtagcac
    15361 ctttttgatt tcaatttttg ccagaggtta tcgactagat accagtagcg gctttcaatt
    15421 aaaagctacc ggacttcttt ctgtcacctc aaaaccaaaa ctagccagtg tttacgttaa
    15481 taatttactt aaaaccgtca ctgatgacac catcaatctc ccccctggca gttatcatct
    15541 taaaataacc aaaaatggtt attatccttg ggagaaagag atccaaatca aacctgagct
    15601 tgtcaatcaa gtcgagattc aactttttcg ttcttcaact gagttaaaac ccttgaccga
    15661 gcatcaaatt attaatcctg ctgccagccc tgatggacaa aaaattgtct acgcccttgc
    15721 ttcaagctca gccacaaaaa aaggaaatgg actctacctg atggagttca cggatcttcc
    15781 acttctaatg agtctaaatt ctgagcgaca attgtttcag agcaccactt ctttagattg
    15841 gtcaaacttt atctttgaat tttccccaga ttccaaacag attttagcca cttcaaccaa
    15901 aaaatcccta gtttatcttt tttctctaga tcagaaccaa aatgtaaata acttatctga
    15961 tatctcgcca catttacctc aaatcaaaaa agagtgggaa ctgagccgag aaaagattgt
    16021 caaaaataaa ttaaccaaaa ttcctgcgga ttttcaacca tttatagcta caaattcagc
    16081 catcttattg ctaaatacca gcgaggataa gcttctttat cagcctgcta ttgacggcca
    16141 gctacctatt cttcttccca gcccaccccc aactcaaagt actcaaaatc aacaacgtaa
    16201 tcttaatcgg ggctcctttt atgtctatga ttttaaagaa gataccaatt tcctaatagg
    16261 ttctaccact ctcagtcaaa tttcctggat tccctattca aataatcttc tctttactga
    16321 aaataatcag attcgagtct gtgattatga tgccaccaat taccaaacaa tatattccga
    16381 ttcttcttct ccaaaaatcc tcctaccaac cccagacggc tatcgtttaa tcatccctcg
    16441 gaacactctt gactcaatta ctttaagaga ccgttagaaa ttttattgac agatattgtt
    16501 ctttttttct ccaatttcac caaggaaagc ctcaacttct tcttcattaa ggtttccctc
    16561 ttcgtagcca agataaattt cgtcttccat catctgaatt tcctcttgac taaaacaaat
    16621 ttcaagttga tcataaggag cttcaagccc ggatactact tgagatggct cctcaattat
    16681 gtcttcagtt ttttctccca aaacctgagt ttccattaaa tactgctctt gcatttgacc
    16741 aacttcaata aaacgaagtg ggattaaagc aaaaattgca atcaaaaaac taacaatcaa
    16801 ggttctggtg tttctgttta aattatccat attaaccagt gtaagtattt attaaaatat
    16861 tgtcaataac atacaaagat ataacctaat cattcaaata atctcatagt tatcttgctt
    16921 atcaataacc aatcaagact gctaactatc ccaaacaaaa aataagataa atataacaat
    16981 atataaaaca agggtactta ttaatacaac tattttacgt gataaaatat ctagatcctt
    17041 aaaaatatcg gggattggcg caattggcta gcgcgcacgc atcgggtgcg tgaggctacg
    17101 agttcgaatc tcgtatcccc gacnntttag aatttggatt ttagaatttt tctgtatttt
    17161 gtccgtcctt atagttcaat ggatagaacg gctgccccct aagcggcaaa tccaggtccg
    17221 attcctggta gggacacaca taaccgcata ttttaaacta aatcaatatc aaatcaccgc
    17281 gaatttttca tattaaccga attaatctcc gcattatatt atatatttta ataataaatt
    17341 tttaccgcat aattaaacta aaattttaag ataaacaaaa tcattaaata ataatctata
    17401 tcatattaac cgcaattgaa tgtcaaatca ctttttaaac taccaaaaac cagttcaccc
    17461 tgaatcatat taatgtctta actatcttca cttttctttc gtcacttaaa gtacatcaaa
    17521 cattactaat tttaatattt aaaaaaatta aaattattat ttatccacac atattttata
    17581 ctaattatat ttttgaatta ctggtcgata ccacaaatat tatattttat tatcctttag
    17641 cactaaatta ggaaattgct aaaatattaa aaattattcc ttaatcacca aatttaacat
    17701 tttttctggc actaaaatct ctttctctat cttttttccc aaaagccact ttttaatttt
    17761 atcgtttttt tctaccagtc tcattatata ttccttatcg cccgccctat ctctttctat
    17821 ctccagtttt tctctaactt ttccatttat agccactggc aatattacca tctcgcttat
    17881 taataattct gaactatact caggccagct actttcatga acagaactgg tttcacccaa
    17941 ctttttccaa gcttcctcag ctgtataagg cgccatagga gcaagtagtt gaactaactt
    18001 ctttaaatcg cctttaccgg ccttatcaat ctcatttacc gcctccatca ttttggcaat
    18061 cgctgtatta aatccaaaat tatcaagatc attacttact ccctttattg ttttatttat
    18121 tatcaatctt gtactattac tgctatcact catatattta atttgcttat caataaaact
    18181 gttaaaccta tcaataaacc tctttacccc cattaatgtt ttttcattcc aagccatcgt
    18241 accagtaaat ggacccataa acatcaaata aagtcgcagc acatcgactc catacttatc
    18301 tgcaattgtt tctggcacaa ttacattccc ttttgatttg ctcattcttt gattatcaga
    18361 acctaaaatt actccatggg aaatgcgttt aaaatagggc tcaggaattt ccttgggcac
    18421 cactccaata tcatatagaa attgataaat aaatcttgaa tatagcaaat gaagagtatt
    18481 atgttcgtct cccccaatat aaatatcaac cggcagccat ttcttnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnngcccagt ttggcatcgt atcagtttct ctctttgcct
    18661 ctccaccaca ttttggacaa ttacatctaa taaaattatc tatttttgac agtggactct
    18721 gaccatcatc agttggctcg taggcctcaa tctcaggtaa ttctaccggt aaattttctt
    18781 ctttttcagg gacccaaccg catttactac aaaaaatcat cggtattggc tctccccaat
    18841 aatgttgccg gctaaaaatc cagtctcgca gatgataaga ggcgcttctt tctccaattt
    18901 tcttgtcttc tatccatttc accatttttt cataaccatc ttttttctca atcacaggca
    18961 ctattggtaa tccaaatttt ttggcaaatt catgatccct ctcatcacag gctggtactg
    19021 ccattatcgc tccagtccca taagatgcca acacaaaatc agagatccaa attggtattt
    19081 tcttattagt aaagggattt atggcatata aaccagaaaa cactcccgtt ttgtctttat
    19141 ttatttcact tctttcaaac tcactttttt ttcttgtttc ttttaaataa tttctaattt
    19201 ctactttact aattaacttt ttaactaatt cgtgttccgg agccatcacc ataaaagtaa
    19261 ccccaggcag tgtatctggc ctcgttgtaa acacctctaa agtttttgtt tttaattgat
    19321 aattgttaat tgataattga aaattgattc tcgctccttc actttttcca atccagttta
    19381 tctgagcctg tttaacctta tcaataaaat ccgttttttc tagtccttct attagtttat
    19441 ctgcgtattt tgtaatattt accacccatt gagaaatagt tcttctctca acttctccac
    19501 cacatctctc acactttcca tttaccacct cttcattagc cagaccaatt ttgcagcttg
    19561 gacaccaatt tatcggcatt tcctccttgt gagccaaacc atttttaaaa agttgaataa
    19621 aaatccactg agtccattta taataatcag gatccgttgt gttcacctct ctttcccaat
    19681 caaaagaata ggccagcttt ttcatttgac tcttaaacat ttctgtattc tctctggtta
    19741 cttcctgggg ttttctttta ctcttaatcg cataattttc tgtcggcaat ccaaacgcat
    19801 cccagcccat cggaaataat anntcttaaa tttttccgta ttctccctag tcacttcctg
    19861 aggttttctt ttagttttta tcgcataatt ctcagttggc agcccaaaag catcccagcc
    19921 catcggaaat aataccttgt tcccttgcat tcttttatac ctggcataaa tatcagcacc
    19981 ggtaaaagaa aaagcatggc caacatgcaa accactgcca ctgggatatg gaaactccac
    20041 taaaatgtat ttcttaggct tttttgaatc atcaccctcg gctcgccata gcgaagcgtc
    20101 ggcggcccag tacttttgcc atttggc
//
LOCUS       DHGB01000003            8972 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_71177, whole genome shotgun sequence.
ACCESSION   DHGB01000003 DHGB01000000
VERSION     DHGB01000003.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_71177"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         2699 a         2082 c         1274 g         2917 t
ORIGIN      
        1 ctagccaatc tttttcgtca ccttccagca tatctccttc ctctccatca agccaatttt
       61 ctttgttagg cttaggcatt tttcctattt ctttattcca ggcatcatct cttaacactg
      121 aatccagcat tattcccatc tgttttgcta aaggtacctt actgattgtc tcaacaaaat
      181 catcaagaac atccattcga tcatctctca taggaacttt cgtgtccagc attccattta
      241 aaaccaatag acccgatcgt ttggatttat tagtttcatt aataattttt tcaacttgat
      301 ttaaatcagc ttgtccagac ccaaaatcaa catgggaaat aaaatgaaca caagttggta
      361 atcccaattt tcctacatcc accctccagt ttaatttact atacagtttt gactctattt
      421 cctgaccccc acttctttct gccaccgaag agttttcctc atcaagagtt aactgaagtt
      481 ctccgtcttc attttctaat tcttgcctta attcttccgt catcccgagt gattcacttc
      541 gatttaatac ttcaaacgcc tccagtaatc ttctcgattt ctcatttccg taagctggca
      601 ccacctgaat ctgattttct tcttttttgt gtcttattac catcgctgct ccaggtggtt
      661 gaattagaac tcctcctttt tctaccataa aaaaatcagg attttcatca ctcaaaccct
      721 taactgttcc tccctgtagc agcactagtt gaacttccgt atttcccgcc aacactcttt
      781 cgctggaaac tcctgcccga ctatggttat gcccagccaa aaccactttt ggtactttct
      841 gatctctgtc tattttttta ttcaaaatta ctcctctgtg tggttttact ggattaagtg
      901 tcgaaccaga tcctccaact ttatgaaaca attgaattcc cacctcggga gaatctccaa
      961 ttttgacaaa aagtcttcca tcgttcatta tcaaaggaat ttttgaacca tcgggtaaag
     1021 tcaaaggatt gagcattatt gctggtccgt ttaacgattc tttaccattt ggccagtcat
     1081 catgacttcc atatcttgaa acaacggcta acactttttt ttcctcaact tttttactca
     1141 aataaactcc tctaaaaaca tcgatttgtt cttgaaaatt aaacatagtt cctgtcgttg
     1201 ttgacatata ttcttgcttt atcccctcca ataaatcacc agctaatatt gcataagtat
     1261 caggtttatc cagttccctc ataatctctt taattgattc gattccggaa gcttctgaac
     1321 caaaatgtaa atctgaaatt agcataatcc tggctacact atttggattt ttgattgtca
     1381 cctttgcttc aatttgatct ggtcttattc tatccttgag cgtttgacca gctttcatgt
     1441 acgattccac catttgtctt gtctttctta tttcacttgg cttatacgga tcttttttta
     1501 ctccttcaaa ttcaattccc gacatccttt aaatcttaaa aatctattgc tattttttca
     1561 atccctatct ttattttttt gattttgtta ttatgaaata gccaacaatt tcccttacct
     1621 ttttgtaatc tttcttactc atttttctgt tttttttatt taaataaatt cccccacgaa
     1681 taagatataa ttcacaccat gcaggtggaa tctcttcatt taagccccat tgaaaaagaa
     1741 cttaagttta atgattggcg gaaaaataat aacattaaat tatttctctt agatttggac
     1801 gatactcttt gtgatactca atcagttttt tttaatcaaa tctcgaaagc ttgtgattat
     1861 ttagccactc attctccccc tctcccaaaa agcgcctggg aaaaacaaat tagaaatttt
     1921 aatgatcagt tttttgaaac atacggcgtc aacccaaaca gatggaattt agttgttgat
     1981 agcctcagtt ctaaatatct tttaccctcc gaagttgaca gtcacgtcaa agaaattttt
     2041 caaaatattt acaccactcc acttgaattt aaagctggag ccaaagaaag tctcaatttc
     2101 ttcaaagcca ctgacacccc cctagctatt gtcacccacg ccaacaaaga ctggacctgg
     2161 cgcaaatata actggctagg gctaaatcaa tatttaaact gggatagcgt ttttattgtt
     2221 gatgaaaatg gacataaaac agccgattcc tggcaatcag ccttagatta tttcaaaaca
     2281 aaagccagcg aatgcgccgt ggttggcgac tctccccgct ctgatatcaa tcccgccagt
     2341 agccttggca tcaatcattg ctttttaatc aaaaattcaa attcttggtc tatccacgag
     2401 cagccggtcc cttctcagac aatcataatt aacaatctca gtgaaatagc taccgtcatc
     2461 aaaaaataaa ttgtcatcag ctactattta tagtatctta tatatacata gtcgccatta
     2521 attttttaaa caaatatgtc gaaatatatc attgacggtg gaaatccttt aactggtgaa
     2581 gtttttatca gaggtgccaa aaacgctagc tacaaacaaa taattgcctc catgctcact
     2641 tctgatccaa ttgaactaat caatgtcccc caaatttccg atgtcaagat taccaaaagt
     2701 attgctgccc atctaggggc aaaaattgaa gaacttggag aacactctct ttcgattcaa
     2761 acaaaaaata ttactgatac ggttattcca agtggtaccg gagaaaaatc aagaacctcc
     2821 ttcatctttt caggccctct tttagctcgc tgtggccaaa tcacctttcc ctcccctggt
     2881 ggcgacaaga ttggcgaaag acctctcgat cgtctttttg cttgtttaaa gcagatgaat
     2941 gtcgagattt tatctgatga aaattcctat accctcaaaa ccagtggtct tatcggcact
     3001 gattacactt tccccaagcc ctctcatacg tccactgaag ttattttaat gactgcagtc
     3061 actgccaaag gcgagactat tattcgaaat gccgcctgtg agcctgaaat tgatgatctc
     3121 attgaactat taaataaaat gggaggtaaa attaaacggg atgaaaacaa tccagccctt
     3181 atttatatta atggagtaaa aaatctccat tccgcttctc atcaaataat ttctgaccgc
     3241 aacgaagccg ttacctttgc ctgcggagct ctgagcacca aaggctctgt cagtatttta
     3301 agaattaatc ccaaaactat ctatactttt ttaaaaacga ttgaaaaaat gggagccaaa
     3361 acaaattggg gtaaagacga ggtttttgtt tcttgggaaa aacctttgac tgctatttcc
     3421 atcgaaaccg agccagaacc aggctttatg actgactggc aggctgtttt ttcattagtc
     3481 ttaacccaat ctgtcggctg tagtagcatc atcgagagag tttttcccag tcgctttcaa
     3541 cacattaaat tcttaaatga aatgggggcc aaaaccaaat ttttccagcc aaaagttagt
     3601 gaccccgcca cttattacca cttcaatccc gaaagtgaca aacctgaata ctttcacgga
     3661 gtcaaaatct acggtccttc aaaacttcac gccaccaatt taaccgttgc tgatctccgc
     3721 gccggggcga ccagcaccct ggccgctcta accgctgagg gacagtccgc tattgatgga
     3781 gttgaataca tcgagcgagg ctacgaaaaa ctagccgaaa gactctcctc cctcggcgcg
     3841 aaaataaaat atatcaagat ctaaaccaat aaatttttac aaaaatttgc tcaaatctac
     3901 tattaatgtc gctaaaattc ccactccccc cacaattaac cactgataaa aattaagcgg
     3961 aactgtccct aaaattttat taaaaaacgg aacataaatc cccataaaaa ccagcccaaa
     4021 accataaatt accgataaaa ataaaacctt atttctaaaa aatttttctg tttttacgat
     4081 tggccttttt aggtttttat atgcaaacac ataaattaaa tcgacagtag ccaccgtcgc
     4141 aaaaaccatg cttctcccca aaactaaatc atgaagaata atcgaataat aatagaaaat
     4201 aaaaagggat aagccaccaa ccagcaaact aatcactaaa ataatatttc tcattctttt
     4261 tggtaaaata tcttcttttt taattgcctc tggtctctgt ttcattaaat caagttcttt
     4321 tgattcaaat ccgagagcaa tatctggtgg tccatcacaa attaaatgaa cccacaaaat
     4381 ttgaactatc gttagcggtg ccggaattct cagtatcgtc gccccgagta ttaaaaagat
     4441 ttcaacaaaa gaatttgaca gaacataggc taccactttt tttaaattgg taaaaattct
     4501 cctcccctct tcaaccgccg aaactatcgt tttaaaattt ccatcaagaa ggattaaatc
     4561 tgctgcctcc ttagccacct ccgtaccact gcccatcacc accccaatat cagccttttt
     4621 caaagccggc gcgtcattga ccccatcacc agtcatcgcc acgatttccc catttttttg
     4681 taaagccttt acaatccgga gtttttgatt gggggtgatt ctcgaaaaaa tgattatctc
     4741 ctcaactctc gccaacaatt cttcttcaga taatttttcc agctctgatc cctcaatcga
     4801 atttgactct gagatatcaa aacctagttg tctacctatt tcctccgccg ttttttgata
     4861 atctccagtt actattttta tctttattcc cgattctagg gctttttgta tcgtttcgcg
     4921 agcatcagcc ctcaaaggat cttcgatgcc acaaaggcca ttccatctaa aatctgtttt
     4981 tttctttaaa tcaccacttt ctttactagc acacccgagt actttgagtc cccttcccgc
     5041 tagtctctca atttctgtca gcactatttt tttcttaatg gcacttatcc ggcaaaaaga
     5101 taaaattacc tccggcgctc caattacaaa agaactttct ttcttacctg attttacaac
     5161 agtcaaagaa tgttttttaa tactgtcaaa gacttcttca taagtttttt ccatagaatt
     5221 aactattttt tgaggattta tctggccttt atttttgaca aaatcccaaa gagctatctc
     5281 caaagaatcc cgctgttcat tagccaaaat cattgacaac tgggccttaa tttcatcttc
     5341 aaaactataa tcaaccacct tcattcgtcc ttccgtcaaa gtccccgttt tatcgctaca
     5401 gataaccgtt gttgacccga gagtttcaat tgagagcagt tttttaacaa ggccttgttt
     5461 tttaagtatt tttctcatcc caatggccaa aataaccgtt accgcgattg gtaaaccttc
     5521 tggaattgcc gccaccgata aaatgatcga cagtctgaga gaatcaaaaa gactcgtccc
     5581 atacagtaat tcaatcacaa agagaagaaa acagacaatc aaaataattt tggccagttg
     5641 tcttgtaaat atttccagtt tattctgaag tggtgtcttt tcttcttcaa tttcagataa
     5701 cgattttcct attttcccca tttctgtttc aattccaatt ttttccactt tcattatccc
     5761 cttgccaagc aataccgtcg tccccataaa taaactattt tgaccttttg ggcctatcga
     5821 tttttctacc gctcgactct caccagtaag cattgattca tcaaccagta ggccatttcc
     5881 ttcgagcaac actccatcac ccggaatttt atcacccgaa tttaagacca caatatcccc
     5941 tggcactagc tcttcaatat taatttcaaa acgtatcccg tttcgaataa ccagggccat
     6001 tggttttaag atatttctta gtgaaagtaa agtttgttgg ctactcagct cctgaaaata
     6061 acccataaag acattggtca acaccactgc cattaccaga atggcgtcaa gatactcccc
     6121 aaacaaaatc gatatcacaa tcaccgacaa caagatataa ataaaaggac ttttaaactg
     6181 agaaaaaaat atttccacct tggaaaaact gtcttttata ggcaggacat tctttccata
     6241 ttttttctcg agtttctcga tttctttttt tgacaaacca gaatagtgca tagaactatt
     6301 atatatagtt ttgaccaaat tttttgtatc attagacatg tcatctaaac tccgaaatct
     6361 catcaaagcc tttggtcacg cccttaacgg ttggaaaagg ggtctaaaag aaagaaacct
     6421 caaaattcat ttagtcgctg ctattttcgt cttatttgct tccttattac taaaaatatc
     6481 gaccactgaa tttattatta ttttaattct gattgccctc gttatttctg ctgagctttt
     6541 caatactgct attgaagatg tctgtaatac cctcaccatt agcttaaagc ttcaatacgg
     6601 agaaactact attcctcgtg atgttgccgc tggcgcagtg ctaatcatcg cctctatcgc
     6661 cacccttgtt ggtttaatcg tttttatccc gaagctctta accctgcttt gataaagcca
     6721 aaatagacca aatagccaaa gcgctgcaaa aattccaaaa tatcgccaca gtttttttcg
     6781 gtcaatctgc cggtattcct ctttaaaaaa ctttctttta tactcgacat aagctacaat
     6841 caccgccagc gatgctactc caagagacaa ggctccaagc acatctgtcg tccaatggac
     6901 acccaaatag attcttgata atcccaggag gaaaatataa gtagaaccta atataaacac
     6961 ccctattctc gcccattttt gataaaaatg acgcacccaa aaataagtca aaagaccata
     7021 aaaagccacc gcaaaataac tgtgaccgct gggaaaagca aaaccatgtt cgagtatcag
     7081 agcattttct actggcggtc gagcccgact gattaaattt tttgccatac taactatcaa
     7141 cattgccgac acattactca aaatcatagc catcaggtaa cgtctttttt gggcaattat
     7201 cagcagtatc gccccaagca ggctccccca cattatcatt tgccaatttc ccgtcaaggt
     7261 tacaagcaac ataaacctat ttgacagtgg ccttcttaat tgaacaacca tttggttaat
     7321 cggccggtca agcctcatca gaccatcttg atcaagataa ccatcgagca cttttccaaa
     7381 aataaccact gccaatagaa atattattgc gaggcctagc catattttat tgagccagac
     7441 ttttttaaac cacataaaga gattataaat taattcctct ttttaagtta tgatattaat
     7501 atgctttctg tcttttttat tctattaagt ttatttgttc ttgttagatc agccgaaatc
     7561 tttattgatc aggcttcagc cctcgccaaa aaattaaaag ttagtgactt tattatcggt
     7621 ttcaccgtgg ttgcctttgg cacctctatc cccgagttaa tttcaacgat tttttcagct
     7681 atttcaggac acaatcagct agctatttcc aacattattg ggagtaatat cacaaatttt
     7741 tgtttagttt taggtgttat tgccatattc aataatttta aaattagaaa aagagatgtt
     7801 gacattaata tccctttcaa catcgttgcc ctcatggctt tttgggctct ttccgcctat
     7861 ttaaacttcg atttaaactg ggccgctgga atctcccttc tctcagtttt tattctctta
     7921 aatattttgt ccaaagacta caaccacctt caaatatctc ataaaaacta ttctcagttt
     7981 aaacccttac tactattaat ctcgcttgct tttttaatcg tctccggcaa aatttgcatt
     8041 gatcaaatta ttgaactatc cattcatttt aaaatttctg aaactatttt aggttatttt
     8101 ctcctggccc tcggcacctc tctccccgaa ctagtaactg tctggatggc tataaaaaaa
     8161 cacgacaaag aactcggtat cggtaatatc ttaggttcaa atttatttaa tctccttttt
     8221 atcattggta tttctacctt tattcaccct ctcgacttta ctgccttcaa aatcgatctc
     8281 ttttttctga ccggcattac cctcgccgtc tatttttatg ctattgttgg caaaaaatat
     8341 tcatttagtc gcaaagaagg tctcggcctt ctttttcttt atcttgtctt tgtcatcttt
     8401 caagccacaa gaatctaaat tcttacaaac agaattccat caggtataag tttaaacacc
     8461 cataaaggcg accaataatt tcttcgtctt gataatattt aattaaatcg aaattccaac
     8521 taaatcagtc ccgaaaaaag aagaatcaat gaaatagcgg taattgttcc aaatataccc
     8581 acagttttaa ggaatagatt taccgcctct tttttcttac cctcaataaa aagcaagaca
     8641 ggttgataaa aaaacaaaaa tgccatcacc gctacagaca aggttaatag tgataagaaa
     8701 acaaccgggg caaaaaaagt atccggttta ttttttaatg gctctgtcac aaagttcata
     8761 acggtaacac ccagaataat gtaaattgaa gcactcaatg cattgattaa aggattctct
     8821 gacataaata taattatatc aattcttctc cccgccttgc ctgcccgcct ttggagggga
     8881 ctcaatcagg actgctattt gcagaattat ttagttgcag agaaagtgat acttaatcca
     8941 tagtccggta aaaatgctgg tatctctttg tg
//
LOCUS       DHGB01000004            4341 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_109069, whole genome shotgun sequence.
ACCESSION   DHGB01000004 DHGB01000000
VERSION     DHGB01000004.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_109069"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1365 a          851 c          763 g         1362 t
ORIGIN      
        1 ttaccaactc tgattatttc tcacaacaaa actttagctg cccaactcta tcaggaattt
       61 aaagagctct ttccccaaaa taaagtttcc tattttgttt cttattatga ttattaccag
      121 cctgaagctt atttacccgc ttcagatacc tatatcgcca aagaggtgga aattaatgat
      181 cttatcgata aattaagact agaagcgacc tccaatttat tttccggcaa agataatatt
      241 gtgatcgctt ctgtttcctg tatctataat attggtgatc cttgtgaatt tggaggcaaa
      301 actttaaccc taaaaatagg tgatttatgg agtcggcgtc agcttttcga agagttagtt
      361 aaattatttt atattcgcag tgaaatggag tttaaacggg ctcagtttcg agttcggggt
      421 gaaagcatcg aaatctggcc ttcttatgcc gactgggtat taattctaga atttgatcct
      481 gaagggaaaa tagccaaaat taccgaacgc aatccttttt ctggccacga atttgattta
      541 caaagttacc agttattccc cgctaaacaa tttgtcgggg ctagtggctc tgatttaaaa
      601 aatattattg ccaaaataaa aagtgactgc gacaaggaag taaaagactt tgagaaaaaa
      661 ggtcaaatct tagaagcccg ccgccttcat cagcgggttg attatgatat ggaaatgctt
      721 gaggaactcg gttatgtcaa tggtattgaa aattattcta tttactttga accaagtcgc
      781 cgcagtggtg atcctccgta tactctaatt gattattttc actatctttg gggagataat
      841 tttttaacaa taatcgacga gtcccatgtc accatccctc aaattaacgc catgtcagga
      901 ggggattatt ctcgaaaaaa aaccttggtt gattttggtt tccgcctcaa aagtgcctac
      961 gacaatcgtc ccttaaactg gacggaattt tatcagcgcg ttcccaaagt tctttatctt
     1021 tcggccactc ccgcctcctt tgaaattgat gattcaaaaa ataatattgt cgagcaaata
     1081 attcgtccca cgggacttgt tgacccctca attcaaattc gtccgagtat taagcaaatt
     1141 cccgacctca tcgaagaaat taaaaataga gtcattaaaa aagaaagaac tttagttatt
     1201 accttaacta aaaaaatgtc agaggaactg gctacttact tatctaatcc tcaaaaaacc
     1261 ggcctgccct taaaagttgc ttatcttcat tccgatattg aaactttaga aagaaccgaa
     1321 attcttgaca aattgagaaa tggcgatttc gatgttctcg ttgggataaa tttacttcgt
     1381 gaaggtcttg atctacctga ggtctctctg gtcgctattc tagatgccgg caatcagggt
     1441 tttcttcgtt cccgttcttc cctaattcaa atcatgggtc gggcttcacg ccatcttgac
     1501 gggcaggtaa ttttatatac tgatgtagtt tctgaggcta tgaaggaggc aattgaagag
     1561 gtgaatcgcc gtcgtcaaat tcaattaaaa tataatcaag atcatcatat aattcccaaa
     1621 agtatcgtta agaatattcg tccccgtctc atcgacgcta atgtcaaaaa tgtcgttatc
     1681 actccgcttt tagaaataga tcccacctct ctgactccaa atcaacgcaa agctcatatt
     1741 gccaagctca aaaaagaaat gcgaacatat gccatcgatt tggattttga atccgccatt
     1801 aaactcagag ataaaataaa agaaatcgaa tccttataaa aatttattga ggcagtataa
     1861 taagaaaaat atggcaaaag aaaaaattca attaaattta ccaaaacaaa aaaatttaca
     1921 aaatttacca gaattttatc ggcatttttt cgatgaatta aaaaaaaatg gatctactga
     1981 aaatagaact aatttaaata aagcaggaca cgacattatt gaaacttcct gggacgatga
     2041 ttcttatcct cctatttatt aggattttct aaacaattta gaagcacgag tatttgtgat
     2101 ttcaattgct tcttcaaagc tacatcgaca aaccgctcct gttaatgggt tttgatgttc
     2161 gggaataact aataaaactc ccccaaatcc tggtactaat tcatttctag gaaaaatcat
     2221 tctgcattca gggttggggc aacttacaca atctaaactt gttttttctt gagacatgtc
     2281 agcattttat catattatat agtaatatac ccgaagacaa accgaaactt ttcgtctata
     2341 ataaaagatt gtgctagaca aaatcatcgt caaaggagct agggaaaata atctcaaaaa
     2401 cgtctctttt gaatttccta aaaataaatt tgtcgttttt accggtgttt ctggatccgg
     2461 caaatcttcc atggcttttg acactctctt tgctgaaggt cagcgtcgct acatggaatc
     2521 tttatcttct tacgctcgtc aatttttggg aaacatgaaa cgtcccgaaa tagacatgat
     2581 tgagggtctc tctccctcaa ttgccatcaa ccaaaaagcc atttcccata acccccgctc
     2641 gaccgttggc actgtcactg aaatttacga ttatctccgc ttgctctttg ctcgtattgg
     2701 tcaccctcat tgtccaaact gtggtcggga agttatgccc caaaccagca aacaaattgt
     2761 tactcaaata attaatacct taaaacagga agtatccagt actccatctc gcttcttaat
     2821 tctttctcct ttggttcgca acaaggccgg agattttaga ggtttagtcg aaaatcttcg
     2881 caaacaaggt ttcaagtggc ttcgaattga caataaaatt attgatttat atgccgattt
     2941 tggcattgtt aaaagtaata aacatgatat tgacgctgtt tgtgatcgcg tttctgtttc
     3001 caaagaatta ctaaaaaata ctgacaatct ttatagtcgt ttaatcgacg atgtggaatt
     3061 agctctaaaa tttagtaatg gtcaagtaat aatatccaga attgatgacc ctggttttga
     3121 ttttcctgaa aacccaaaag aatttaaaga gacattatat tctgaaaatt ttgcctgtcc
     3181 ggtttgtaat atttccttac ctccaattga accccatctt ttttctttta attctcctca
     3241 gggtgcctgt cctgaatgta agggtctcgg ctctaaatat caaatcgatc gaaccaaatt
     3301 tcctgagtgg aaagccagaa tgctcgaggc ccgttatttt aatgctccct ccgaaggtgt
     3361 ccgcgaagaa ctcgaacagt atatgatcaa agagagttgt ccaatatgtc attcttcccg
     3421 tctcaatcct gaggctcttt cggtcaccat tcttggtcaa aatatttatc aaatttgtca
     3481 attgtcaatc gaaaatttat ttaaatggat aaaaaatcta cccgataaat tagattcttc
     3541 tcaagaaaaa gaagttttag actcaattca acgggaaatt ttctcccgtc tagaattttt
     3601 attagctgtt ggtcttgatt atttatcatt agagagagaa gccggcactt tatcttctgg
     3661 ggaatcgcaa cgaattcgtt tggcctctca aattggtact ggcttaactg gagtattata
     3721 tattttggac gaaccgacta ttggtttaca ttcgagagac aacgatcgac tgattgctac
     3781 cttacaaaaa ctaaaaaatt taggtaacag tgtcgtggtg gtagagcatg acgaggacgt
     3841 tataaaatct gccgattata tcgttgattt tggtccttat gctggcaaaa atggtggtga
     3901 aatagtcgcc gatggttcat tagacgacat taaaagaaat aaaaatagta ttactggaaa
     3961 atatttatca ggcaaattaa ccattaaacc tgtagagacg catcacgatg cgcctctacc
     4021 tttaataaat aatcaaaaaa tcaaaattac cggtgcccgc cagtggaatt taaaaaatat
     4081 taatgtcgag tttcccttaa acaaattaat ttccgttacc ggtgtttccg gttccggcaa
     4141 atcaactttg gttcacgaca ctctttacgg aggtgtccgt aaagctactc tcggatctta
     4201 ttacggcact attggggaat atgacaaaat agagggctgt gaaaatttat cggatgtcct
     4261 cctcgttgat caatcgccga taggtagaac tcctcgttca aatccggcga cttatactaa
     4321 actttttgat gatattcgaa a
//
LOCUS       DHGB01000005            4188 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_27054, whole genome shotgun sequence.
ACCESSION   DHGB01000005 DHGB01000000
VERSION     DHGB01000005.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_27054"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1205 a          649 c          913 g         1421 t
ORIGIN      
        1 accgacgata accggagttg gtttgacata gattgataca taaatatagc caatagtatc
       61 gatttattta atttgatata tatattagca gaaagatata gaaagtgcta atagaataga
      121 taaatagatt agaagattaa tagattagta gttaaaaaag attgaaggca gtgagatgga
      181 cttttttggc ggttttgtca tcgcagttgt aatatgaaac ccggaaagta tagcgatttt
      241 tataaatatc aataatttgc attgaccata aaatttttga gtttattttg gcagaatatt
      301 ctttaataag attgattgga tctttgggtg aattaagatt taagttggcg tcgagggtaa
      361 ttatttgaga agttaactcg atagcctggg aaggtaaatt atggggaaca tatttattgg
      421 gtttaggtaa attatctaga atgatttctg aaaagttgtt atcgataatg tctgtagtta
      481 ggtttaatgt gtttaaatcg gattgtaatt tttgtgaatc gtagttgtag attcccagag
      541 cgttttcttc aaaatactta ccgttttctt gaaaataaat cttgccaatt tcaaaaaact
      601 gagcttggtg taatttgaat ttttgatact gacttaattg cgattttaat gattgaatta
      661 tcgattgacg aagatagatg acttcactgt ttatactgtt ttgagtgtgg attgcttcgt
      721 cgtcaggctc ctcgcaatga ctagtcagtg gccaggacat gatttcatcg tagcctaggg
      781 caactaattt atcttttaat tcatcgatca gataaataat ttttggggta atatcgggta
      841 attttttaaa agttaaaggc tgactcatgg gaattttttg gtagccccag aaacggacga
      901 cttcttcgac aaggtcggct tcgatatcaa tatctttgcg gatggaggga gggtttactg
      961 agaaactgga ttctagattc tggacgatag aacagccaag gtgtttgagg acgttgatgg
     1021 aaaaatcggt gggaatgtca attccggcga tagcagatac tttttgagga ttaaattcaa
     1081 ttttaggagg agaaatttta cctcgataat aatcaaaaag tgaggaagag atttgaccgc
     1141 cacagttttc taaaattaat ttgattagat gattaaaagc ggtgggaatt agttcgggat
     1201 ctaaatcttt ttcgaggcgg atacttgact cagttacact tttgagctgg tggctatttt
     1261 cacggacagt ggtggggtca taaagggcca tttcaatgat ggtgtcggtg gtggaagtgg
     1321 taatagcgca ggcctgtcca ccccagaaag agagacttag agcattgcct ggattgttaa
     1381 tcattaatat attgttatcg agatttaatt tagttttatc gaggctgata aactctgaat
     1441 atttagaatt tatttcccaa attaaattgt cggtagattt ggcagtatcg aaagcatgaa
     1501 caggaatacc ataaagaaac ataatatagt ttgttaaatc aactaaagtg tttaccgaat
     1561 tggtgccgtg aagtttaata aatttttgaa gccagtcagg tgaagtagaa ttttttaacg
     1621 cagtgatttt taaagccatt attcggcgaa ctttgtcggt tgtgactgcc aagggaaggg
     1681 aataattaga gaccggaaaa gaagggctag actccttgga aagaggaaga ttgtaaagaa
     1741 cagaaaggtc gcgggcgaga ccgtagtagc caagacagtc gcctctgttt actttaatat
     1801 ctagatcaaa aactatttca ctttgagttt cttcaaaata attagtgaag tgaccaatca
     1861 tggtcaaatc atcgcgtaat ttctctggat ttaccgataa gtcaggaaga aattttttta
     1921 attctgaata aattagtttc ataagttagg tttgtaggcg aggttgcctc cccgaagagt
     1981 gcgaatatct ttaatgttgt ttttggccat agtccagcgg tctagcccca tgccaaaggc
     2041 aaaaccggac catatcttgg gatcgattcc ggcggctttc aagacattgg gatgaattat
     2101 gccactacct cccatttcaa tccagccggc gtatttacac agagaacaac ctttgccatg
     2161 acaggaaaaa cattgcatat caggtccaac ccctggttcg acctcaggat aatatttaca
     2221 gcgataacga acaattacct tgggtccata gagggtttta aacattaaat ccatagtgcc
     2281 aattaaatct ttcatggtaa cgtttttgtc gacaactacg gcttgatatt gttgaaaaat
     2341 aaaatgattg cttcgattga ccttttcgtt tcggtaaaca gaaccgggaa aagcggctcg
     2401 gataggcaaa tttttatttt gggcttgaag taaatatgat tcgatagttg aggtctgagt
     2461 tctgagtaaa aactgaggtt ctttaatata gatagtatcc tgcatatcac gggctggatg
     2521 attggcggga acattgaggc gggtaaaatt gtattcgtcg gtgacgattt cggggctagc
     2581 ataaatagaa aaacccagct tttgaaaaac tttattgagt tggcgttcgg tttgagtcag
     2641 agggtggagg tggcccacct tcgctaaagc tacggcgggc atgcccattt ttagttgaga
     2701 tgttgtgttt gagatctcta cattaatatt tttatttttt attaactgct caatgtcatt
     2761 ttttaattga ttcagtttta gcccatattc acgtggattt tcgatatttt taatttcaga
     2821 aaataatcta ttgattaaac ccgagcgacc gagtagttca attcgaatat tttctaattc
     2881 agccgaagtc ttggcccaat cgattttatt ttttgtttct tttagtactt tgtctaaatc
     2941 catttatata cgtttatatt aacaaaaaac cccgcggttg cggggtgtct aatagttata
     3001 aattgccccc cgcctgcagt cgctatgcgt cagcattgcg ggcaggctat tttaaggaag
     3061 taaagataga tttaaaggta gacggatcat ggatagcgat ttcgctgagc atcttacgat
     3121 ttaactcaac cttattatct ttgagttttt ttataaaggt ggagtagcta agagaaaaag
     3181 gacgaagagc cgcgttgatt ctgacaatcc agacttttct gatgtcgcgg cggcggatac
     3241 ggcggccatt gtaggcatac tggccggcgt gtaaaactgc ttcggaggcg actttataac
     3301 gcttggagcg agtcatccaa taacctttgg cctgttttaa gatttttttg tgggcgtctc
     3361 gtctgacggt tccggtttta actctcatag atttagatta atagataaat agattaatag
     3421 ataattattt tttgcctaaa agctttttaa ttttggtagc cagtcgaccg gtaacaactt
     3481 ttttgccttt tagacgacgt aattgttttt tacttttttt gcgacgaaga tgacgtttga
     3541 aggaggactc gcgcataact ttaccgtttt tggtgacggt gaatcgattg ctaactgatt
     3601 tttttacttt atgtttttta cttgcttttt ccatattatt tcttggtttt tttgactgga
     3661 gtaaaagtaa taaggagtct tttacctatc aatttggcgc taccttctgg ggcagaaaac
     3721 tcagctaaat catttttaaa cctttcgatt agctgatgac caaattcagg cttttgaatt
     3781 tggcgacctt ggaattttat acttaatttg actttatttc cagtagctaa aaattctttg
     3841 gcttttttaa ttctggtatc gtaatctccc tggccgataa acggggtcat ttgaatctct
     3901 ttggtttcac cgcccttgag accttttttt tcagctttgg cttttttggc ttcctgatat
     3961 ttaaatttag aatattcaat taattttact accggcggtt tggctgagcc ggaaatttcg
     4021 actaaatcta ggccagattc ttgagagagg ttgatggcct cagcgagaga taaaatatca
     4081 atttgtttac cgtcttcatc taaaagacga agagttggag cggtaatttg tcgattaatt
     4141 ctgtagaact tttgagtcat tgagttattt tatcagattt aaggactt
//
LOCUS       DHGB01000006            4327 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_112084, whole genome shotgun sequence.
ACCESSION   DHGB01000006 DHGB01000000
VERSION     DHGB01000006.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_112084"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1446 a          699 c          814 g         1368 t
ORIGIN      
        1 ggtaatggcc gaatattttt tgccaatatt tgaaaatctg aaacttttag ggtaatttca
       61 ccagcctgcg ttttgaatat ctcaccggaa gcagaaatta aatcaccaat atcaaatagt
      121 ttggttaaat caaatttctc ttttaaaata tcactttgaa aaaatagctg aattttacct
      181 gttctgtcga ctaaatcacc aaaaagtatt tgaccatggc cacgaagact cattaggcga
      241 ccggctaaaa cgacgttttg gccaagcatt ttttgagctt cgagacagtt attttttgac
      301 tttaaatttg gttgaggata ggcatcgaaa ccaagcgatt ttaacttttc gagcttatca
      361 agacgaattt gccttaagtc ggacatgctt tattttatca agctattgag acaattttgt
      421 aggttagttt acccgcaggc gcgtcaactt caaatttctc gttgaccttt ttcccgatta
      481 gggccagacc aagaggagac tgagatgata ttttaccgga agctggatca gcctctagct
      541 caccaacaac ataaaaaatt ctttcagagt ttcctaatat ttttatttta acctttgagc
      601 cgacttgaac ggtattagaa ttattttttc cagaaataat ttcaaaattt tctaaaacag
      661 attcaattga ggcaattttg gaattaacta cttctagctc tgcttttagc tgtgaaacca
      721 atgaatcctc tcccgactcg tcgggctgag ctacttcttc aattttggta atcaggcggt
      781 cagcccagtc ttttaagctg gctagttcgg aaactaactg tgcgtgacct tctttggtta
      841 gttgattctt tgtcatagct ttatacgaaa agccctcagc gtaatgattc tgagggaaat
      901 aaagagtgac aagttaattt tttgtcacag gtgatatggt aaactagaag gtaaattttg
      961 tcaaatataa gtgttaaatt tttttagggt agttccaaaa aatcgtaaga ttagggtaaa
     1021 taaatctaat ggtttacttt actcaaatcc cccaacctca aaaagggtta tcttttattt
     1081 tggaaatatt ttatttttag gagccataat ttacggagga tatttatatg cacccttttt
     1141 aaaagctttt tataactaca agttcatcaa aacagaaact aaggttgaaa caattggtga
     1201 acaaaaaata gaaatacaac aatctgacga attttttatt caaattccca aaatactagc
     1261 tttttctaaa gtaatcgaaa acgtgtctcc ttttgatcag gctgagtatc taaaagtcct
     1321 gaaacaaaat ctaatcgctc atagcaaaaa tagtgatagc cccggaagtg gtatcggaag
     1381 aatgacctat atctttgctc attcttcgat ccgagaaatt gaagacgtaa ggaacaatcc
     1441 cgttttttat ttgctgggag agcttgaact taacgatgat atttttataa aatatcacgg
     1501 ccaggaatat aaatatcggg tttatgataa aaaggtggtc aaggcaaatc aggttgaatt
     1561 tttgaattac cgagatgaaa ataaagaact actcatcctc caaacttgct ggccgattgg
     1621 caccgactgg aataggctat taatttttgc agaactaacg acatgatttt agatttatcc
     1681 ttaaatcagc ttaatcatga ttattgggaa gaattgaaag gggttcttga aaagaataag
     1741 attgaaagtg aaaagataaa aatcgctttt gattttgcct ataaggctca tgagggtcaa
     1801 attagagagt cgggagagcc ttatatttca catccggttt gggtggcaaa attaatagca
     1861 cagatggaaa ttggtcaaga ggcggtaatt gccgctctaa tccacgactg cctaaaaaaa
     1921 acttcagcga caattgaaga aattgatgaa aaatttgggg atgaggtggc tttgcttgtc
     1981 tcgggactag gtgaggttca aaataaaacc accggtaaaa atattgacaa caccaatgtt
     2041 gagattttta gaagattttt attttcttcg gttgatgatg ttcgaatttt aattatccga
     2101 ctggccgata aacttcataa cggcttgacc atcgatagtc ttccgaagga aaaacaaatc
     2161 gaatatgcca aaagagtaat gggaatttat gcccctattg ccgaatatgt gggattgcat
     2221 tatttcaaaa aaagactcga tgatattgct tttaggattc tctatccaaa ggaagccgct
     2281 gagctaagcg aagaattaaa aaaacaagaa aaagacgaaa ttaaggcggt aactctcgtt
     2341 aaaacagaaa ttgaggaaat gcttaaaatt aataatatta ataattttga aattcaaagc
     2401 cgaatcaaaa gcctgtacag cacttatttg aaaattaaaa gaaaaaaagt ggataagatt
     2461 ggtgataggg tgggaattag aatattaact gacacggtat caagctgcta tacgattttg
     2521 ggtttacttc atgccagata taaatatcta ccggatgaat ttgatgatta tatttccatt
     2581 ccaaaatcaa acggctatcg ttctatccaa acaacattga actggaaaaa taagataaca
     2641 gttgaagttc aaattagaac gagacaaatg catgaattta atgagtttgg tccggcatct
     2701 cacattgctt ataaactagg gaaaaatagc gagagtggtc tcggaataga ctgggttaaa
     2761 gatctagtta aatggcaaaa aagtgataac aatgttaata attacaaaat aaaagtgctt
     2821 agccgctatg tttatgtctt taccccaaaa ggagacacga ttcagctacc aaaaggatca
     2881 actgctctcg attttgccta tcgaattcat accgatctcg gagaccattg ccatggggct
     2941 aaaataaata ataaaatggt tcgaattgat caaaaattaa atagtggtga tgtcgtcgaa
     3001 atattaacca caaaaaataa aaatgtctgt aaaaattggc tggatattgt aaccacgagc
     3061 tttgccaagg agcacattag aaaaatgaca tcagagagcg agagatttgc tgattaattc
     3121 ctgacgatat ttattagcaa aacggtgagc ttcatcccga agtttcatca ataaaagcag
     3181 ggcttgagat tttggcggaa ggttcatttc tatccaagaa tctccatttt taataactat
     3241 agtttcaatt cttttagcta ggccgattag gggaatggat tgcttcgctt tgctcgcaat
     3301 gacgttgtca acagcagaca cctgtggctt tcctccatca accacaatta aatcaggagt
     3361 cccccactcg gggtgtcgga gacgacggta aacaacttct ttcatcatta gaggatcatt
     3421 agattgccac gtcgcatcgc tcctcgcaat gactttatta aatttgtttt taattttaaa
     3481 ttttttatat tcatttttat caatctcacc attttgccaa acaaccatcg aaccaacaaa
     3541 gtatttgttt cccagttgag aaatatcaaa gcactcaatt cgacgaatat ttttaatttt
     3601 taaatatggt tttaatgtag tctcaagact ttcaatagcc tgactttgct tatcctctgg
     3661 aagatttata tcggcgtata aattttttaa agattgccaa ccggaaacta tgtaatttaa
     3721 tgaatcaatt tgatttttta aaattaaagc attttcataa tcctctgatt ttgaagcaat
     3781 tttcattttc ctatttagag aaagaaaaag tgatttaaaa tgtccattta atattttttt
     3841 aatttttata atattttgat gatatatttt gggatcagga ctggaccctg ggcaaagacc
     3901 aaggtggcaa tataaacatt ttgttttagg atgagtttct tgacttcgat agggaaagat
     3961 gcgacgaatg gtttttaaaa gagacttaac tgaggaggcg tcaggaaagg ggccgtaaat
     4021 atcggcattt tggggtaaat tactggaatg agctgtaaaa attataggga tttttgattt
     4081 agaaatacaa atgtaaagat atgatttatt gtccctaagc tgggaattat atttggggtg
     4141 atattttttg atgagagacg attctagaat taaagcctca atttcggagt taactgtttt
     4201 tgtctcgata ctggcaattt gagaaactaa aattttggtt tttggaccaa gggcatcatc
     4261 tcgctgaaaa tattgggaaa cacgtttttt taaattgatg gccttgccaa cataaataat
     4321 ctcccca
//
LOCUS       DHGB01000007            4446 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_69199, whole genome shotgun sequence.
ACCESSION   DHGB01000007 DHGB01000000
VERSION     DHGB01000007.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_69199"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1379 a          873 c          851 g         1341 t
ORIGIN      
        1 ggtaaaaaaa ttactgatat tgccagactt tgttctccaa atattattat agccgacaaa
       61 attacctatt ttttggatcc tgatggtgcc gaaacttttc ctgatttaat gtcacgagct
      121 ggtaatctat tagataaatt aaacatagct tatcaaaata aaaatatttt gttagtcacc
      181 catggtgatt ttggaaaaat gttttttgct ttttattatc atttgggctg gaaagaagta
      241 ttgacctcat tccattttgg taattcagag atgttgattt tatctccaaa atattcccca
      301 aagaatgcga aattaatcag tattaatcaa tataatgcct aaaattatga acaggcaaaa
      361 gtattatgaa gcagtaaaag ctaatttaga caccaatatt ttttatcatt ccctggctct
      421 agaagcctgt ctgggcggta tttacgatta tctcaaatta aataatcaat taggagcann
      481 aggagccgag tcgggatgaa tggactttag ccggtctggt acacgacatt gattatgccg
      541 gggagaccaa agaaactcat ccggcaaaaa ctcaagaagt actggcaaag tttagtctag
      601 aaatttctcc agttgttgat cgtatcgtca aagatcactg tgcttcaacc ggtttacccg
      661 ccaataaagc ggggtgggct attcgctgtg ctgattcatt gaccggtctt atagtcgccg
      721 tagctctggt ttaccccagt aaaaaattgt tagacgttaa ggtttcttct gttttaaaaa
      781 gatttttgaa agaaccccgt tttgccgccg gtactcgccg cgatgaagta attttgtgtc
      841 aagatgcaga taaattagct attccccttg agaaatttat tgaaatttgt ttagaatcaa
      901 tgcggaaaat agcctcagaa attaaattat aagatttctt ttttagactt taggctttag
      961 actttgttct taattatatc ctatcttatg gtaataattt atataattag agacacactg
     1021 agtgcgtaag atatatttag atacaaattg ttacggaaaa acttgcggag aaaagtgcct
     1081 ctagttgtgc taaaataagc ccatctcatg atcgatataa atctcttaag acaacaaccg
     1141 gatctcgtaa aaagggcttt aataaatcgt caaaaagacg tttctatttt agaaaaaatc
     1201 ttagatcagg acaaaagata tcgtgaacta ctgggtgaag tcgaaaaaat tagagcggcg
     1261 caaaatcaaa tttccaagtc ttttagtggt aaacccagtg acgagcaaat atctaccgga
     1321 aaagaattaa agactcaatt aaaagatctt gaggaaaaat taaacattgt agaaatcgat
     1381 ttaaatactc ttcttgaaga agttcctaat cttcctgctg acgatgtccc catcggtaaa
     1441 gacgactctg aaaatgttgt tctaaaaaca gttggtgaaa aacccgtttt tgattttact
     1501 ccaaagcacc atcttgattt aggtgaggct cttgatattc tcgatgtcaa aaaagcggct
     1561 gaaatatctg gttctcgttt tggttatttt aagggccagg gagcagtgct tgaaatgtct
     1621 ttaatgtttt atgctttcaa aaaattgacc gctatgggtt ttattggcat gattccgccg
     1681 gcgatgatta aatccaaaac cgaatggcaa tgtggttacg ccagcaataa gaatttattt
     1741 ggcgcctatt attctattcc cgaagatgac ctgattttta tcagttcctc tgagcatgct
     1801 gttgtccctt atcatcaaaa tgaaatttta aatttaagtg ttttacccct tagatacgtc
     1861 aattattcac cctgttttcg tcgcgaggcg gggacttacg gcaaagatat gaagggaatg
     1921 ctccgtgttc atttttttaa taaagtggaa atgaatgtat ttactctacc cgaccttaaa
     1981 gtttctgacg ctatgtgtct cgaaatgctt tccattgaag aagaaatatt aaaagattta
     2041 ggtctttctt atcaggtaat gcgcaattgt acgggtgatt taccccagcc aaaccgtcgg
     2101 atgtatgatc tcaatacttg gtttcccggt cttgatgaat acaaagaaac cggttcctgt
     2161 agcaactgta ctgattatca ggcccgtcgc ctcaacacca aagtcaaaat cgacggccaa
     2221 aattcttttg tccacatcct taattccact gtcgtcaccg accgggttct tctcgctatt
     2281 ttggagaact tccagaccaa agacggcagt ataaatatcc ctgaggtact ttggcccttt
     2341 accgggttca aagttatctc ccctaaatcg tagaggagcc acccttaccc gccggagttc
     2401 aatctaaaat tgaacaaagg cgggcgaggc ttttttgaca ttaattaagc tataatatct
     2461 ccatgttcaa ttttctaaaa aatttaattg atgataacca gcgccatcta aaagtcttca
     2521 aacctttagt tgccaaaata aatagcttag agccagaatt taaaaaatta tctgataaaa
     2581 aattaaaaaa taaaactatc gaatttaaaa aaagactatc cgatggcgaa aaattagata
     2641 atcttcttcc agaggcctac gcccttgttc gtgaagccat ctttagaagc attggcgaaa
     2701 gagcttatga cgttcaatta atggccgcta tcgctcttca ccagggatca attgccgagc
     2761 aacgtaccgg agagggaaaa actcattccg ttattttccc agcctattta aatgctctca
     2821 ccggccttgg ggttcacatc gttaccccaa atgattattt aaccagggtt ggcgccggtt
     2881 ggtatcccca ggccttaaat ttacttggtg tttctaccgg cgctattatt catgagcagt
     2941 cttttatttt agatcctgag tatactgatc cgagtgaaaa aatagacgat cgtctcgccc
     3001 atcttcgacc tgtctctcga cgcgaagcct atcaggctga tattacttat gggaccaaca
     3061 atgaattcgg ttttgattat cttcgcgatc atatggtggg ggattttaac caaaaagttc
     3121 aacggccaaa tcaccacttt gccattgtcg atgaggtcga ttttgtttta attgacgaag
     3181 ctcgaactcc tctgattatt tcttcaccta acaatcagcc agttgagaaa tactataaat
     3241 ttgcccaaat tgccaaatca ttacagccca atgattatgt catcgatgaa aaatccaaat
     3301 cctcaacctt gtcagaattt ggtatccgaa aggtcgaaaa aatactcggt gttactaacc
     3361 tttacgaaga atcttttgac actattcatt atattgaaaa cgccattaaa gctcaggcct
     3421 tatttcacaa agataaagac tacgtggtca aggacaatga aataattatt gttgatgaat
     3481 ttaccggtcg cctcatgcaa ggccgtcgct ggtctgatgg tcttcatcag gcagttgaag
     3541 ccaaagaaaa cgttaaagtg caacaggaat cacaaacctg ggctactatt acttttcaaa
     3601 attattttcg tttatatcaa aaattagccg gcatgactgg aaccgcggct accgaagccg
     3661 aggaattcaa aaaaatatat aatcttgacg ttataactct tccgaccaat cgacctctaa
     3721 tcagaattga tgacaatgat ctaattttta aaacccagcg aagtaaatat gccgccattg
     3781 ctgccaaggt tgaggagttg catcgtaaag gtcaaccggt tttaatcggt accaccagta
     3841 ttgctaaaaa cgaaataatt tcctccatgc ttcgtaataa aaaaattcct ttccaaatgc
     3901 ttaacgccaa aaaccaccaa tcagaggcag aaattattgc caaggctggt atgatcggag
     3961 cggttactgt cgctaccaac atggccggtc ggggagtcga tattatttta ggcggacctc
     4021 gtgataaatt taccccagaa acatggcaaa aacagcacga agaggtaata ggttttggag
     4081 gtctttatgt tatcgggact gaacgccatg aatcgaggcg tatcgacaat cagctacgag
     4141 gtcgctctgg ccgccagggt gatccaggtt tttctcagtt ttatgtcgct cttgacgatg
     4201 atttgatgag agtctttggg ggtgaaaaaa tagcggagct catgacccgt tttaatatgc
     4261 ccgatgatgc tcccttaact caccctttag tttctcgggt tctggaacaa attcaggtaa
     4321 aagttgaagg ttataatttt gatgttcgaa aatccctagt cgaatacgat gatgttatta
     4381 acaaacagcg tcaaataatt tataaacttc gggatgaaac tctctccgat gccaaaaaca
     4441 atcccg
//
LOCUS       DHGB01000008            3684 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_127173, whole genome shotgun sequence.
ACCESSION   DHGB01000008 DHGB01000000
VERSION     DHGB01000008.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_127173"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    2584..2614
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1146 a          710 c          569 g         1226 t
ORIGIN      
        1 caatcaaatt aaaatatttg acactcaaat tgcccagact cagcttaaaa taaaacaaac
       61 cagctataca attgaaaccc taaaaaaaga cattgctgat ctgggcgtta aaataggtga
      121 tttagacatc gcgcttaatc agctttccgc aatttatatt caggaaatta atcaaaatta
      181 caaactcccc aaaagaatcc ctcttttgag cttaattact tccggctctt ttaacaattt
      241 ttttgaaaat tacaaatata tatcgattat tcaaaaaaat agtcaggata atctcctaaa
      301 tctggaaacc actcggacta atctcgatct tcaaaaacaa gctaaagctg aaaagcaaaa
      361 agagcttgaa gctatggaaa aacagcttga tgcccagcaa aaaagcctcg ccactcaaaa
      421 aagctcaaaa gtcggattac tagaaattac tcgaaacgat gaggctaggt accaaaaatt
      481 aaaagctgct gccgaggaag agttaagctc ccttttaaat gccaccttta aggacaagcg
      541 aaatgtaaaa aagggtgatt taatcgggat gatgggaaat accggttatt cttttggtga
      601 ccatctccat ttcggtctat ataatttaag tgaaagtaat attgcttcat ggtcttatgg
      661 caatgacatt gatgccacgg actatctaaa tcagcaccgc tggccgatga gtggtgttac
      721 ctcaattagt agtacctgta acgcctcaaa tacgacgaat tgtattaccc agcttcgtgg
      781 acacaccccc tattcatatt tatatgccga tcgttttcat catggtcttg atatggtttc
      841 agttaataaa agtgtctacg ctattgagga tggtgtcgcc tatacttatc gcaataccaa
      901 atcatcgcta ggtaaccacg tcaaactatt tcactctgat ggcaagatga ccctttatct
      961 ccatctccaa taaatttact aatctactac tctactaatc tattactcta ttaatctatt
     1021 cttaatatcc tctagctact tttcttaaag ctaaagcgac aatcgataaa cttaaaacta
     1081 tgcctgattt aaccactaaa tcagaattac cagtaaccgg aataaccttt ccatttagat
     1141 aaaaagtggc ggtatctttg tcgtaaacgt tattattttt tacgtcaacg acattagtct
     1201 gagaattcca ggtactggaa ttaattacct tggctctaat ggtaaaagtt ttactttcac
     1261 caggatttaa agtctctatt ttccaaatta tttgacgatt agctttgtta atttctcctg
     1321 gagctaaaat aatttggtta acgactgggt aataatcagt cacgatcagg tcgtaaagaa
     1381 tatctttacc agtattttca actactatcc tgtagtcgac cacatcatca ttaacaaaga
     1441 ctttttgatc cctcccgata ttgtcataaa aattagaatc atttataggt ctaatttttt
     1501 tgtcaatcga gatattataa ttaacttctc cttgagagta agcatcagca aatattccgc
     1561 taggaaaaac caaagcagtt aagagcatta gagataaaat tatttttttc atataaataa
     1621 atgacaataa ctaatattag aggatattat acccaatttt acccgagagt caagtcattt
     1681 gctatacttt tactatgaaa aactacctga ttgacgaact ttctgtattt tttccttgtt
     1741 ataacgaaga aaacaannat tcaggctacc gttgacaaag ccgttagagt tctcaagcaa
     1801 actgctaaaa agtgggaaat agtcattgtt aatgacggtt ccaaggataa aacgggagag
     1861 gtggctttaa agattcaaac aaaatatcca aatataaaaa tcgtcactca caatcccaat
     1921 cgtggctacg gtgccgcctt taaaagtggt ctctataatt cgaagtataa atggattgct
     1981 tttaccgatt ctgacggtca gtttgacttt tctgaaatca aaaattttat tgagactcag
     2041 aaaaagacaa aatcagattt ggttattggc tattacttgg atcgaaaagt gtcaaaggct
     2101 gtcattatta gctctaaaat atgggaatta atcgttttta tcctttttgg actcaaggtt
     2161 aaagatattg attgcggctt taaatttatt agccaaaagg tcgtcgacac cattcctaaa
     2221 ttagaagcgg aaagaggagc ttttatcagt agtgaattcc tcataaaagc caaaaaagcg
     2281 ggatttaaaa ttgtcgaaat cggcgttcat cattatcctc ggactgaggg tcaggcgact
     2341 ggccgccagt ttaaagtcat tgtaaaaagt ttttcagatt tgtttaaact ttggtataaa
     2401 attaatttta cccgtgctta aaaaattctt aactaaaaat tggctgataa ttctaatctt
     2461 attaatagcc tctttttgcc gtctttaccg tatttccggc tatatggaat ttttgggtga
     2521 tcagggccgt gatgtagtta ttgttcgcga ttttctaaaa aatggaaact tattttttat
     2581 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctctt cttttagcaa actatagtcc
     2641 ggttggacca gccatttttg ttgctcttct gggcgtagcc accgcctacc tcattttttt
     2701 tattactaaa aaatggttta atcccaaagc tgcctatatt gccgctttcc tttacgctat
     2761 ctctcccact gctattaaat attccaattt ttcctggaat ccaaacatta tgcctttttt
     2821 tgcccttctt tttatctatt taatgacgga aaaacgctac ctttgggcct gcctcgcctt
     2881 tgctatgtgt ctcaattctc attatctcgc cttgttactc ttgcccactg cttttattat
     2941 ttggttaaaa aattcacccc gaaaatatgt taaagagaca cttctcgcca ttttaatatt
     3001 tatgctttcc cttattcccc aatttctctt tgatctgaag catcaggggc aaaatattaa
     3061 agccttagcg atttttttta cccaaagaga aactacggtc aatctaaaag cctacaaatc
     3121 attacctgtc atcccctcgc tctttaatca aatcaatact gatttattgg ctgggcaaaa
     3181 ttctactcat ggattgatta tttcaattat cctgttttta ggcctaatat atctattaat
     3241 taaacaccga aatcaaaatc tactttactg tgttttgtgg tatttaatcg gcttaattgg
     3301 tcttgggctt tacaagcaac atatttattc ccattacttc ggctttttat ttccggtggt
     3361 ctttatgctt atggcctatt ttttaagtaa acttccaaag ctgttaacta ctattctatt
     3421 aatctactta tctattattc taatatctgc caatcctttt cgctggccac caaacaatca
     3481 attaaaaacg gctaccgaaa ttgttgacca tatcgttgct aaatctggta atcaggattt
     3541 taattatgcc cttttgtcga aaatgggtta cgcttttcca tattatttag ttgaaaacaa
     3601 acattatttt gagattaaag acagattaac cgagcagctt tttgttgttt gtgtccctca
     3661 tcccgacgtt aattgcaatc ctat
//
LOCUS       DHGB01000009            8070 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_74835, whole genome shotgun sequence.
ACCESSION   DHGB01000009 DHGB01000000
VERSION     DHGB01000009.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_74835"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         2554 a         1175 c         1696 g         2643 t
ORIGIN      
        1 gctatagact cgatggagga attataacaa aattttaatt gacacttctg gcagaggaat
       61 gttcactact catcaggtac tgttccccac cttttttact tttggccatt gaccagaaat
      121 aatttctttt tttctttttt ttggtaaaag attcaagcca agaggcagct tcgtttatat
      181 ttttacggaa attggtaata aagtttttga ttctaactaa aaaatttatc tgatattctg
      241 aagcttcggg aatgttttca agactgatat ttttaacatc tttatcaaga tcgtcagttg
      301 attgggttaa tttttcgatt tcttcctgaa gctcggttac aactttttct aactctcgat
      361 ttttttgatt aagcagttgt ttttcttgct gctggggaag atttgggtta cctaaaaatt
      421 ctttggccca atttggagat tcgggtgaat ctttatttaa ttctacggtt tggttaagac
      481 ctaaaattcc actaagggaa aattttttgg gagcagtatt agcctgtgta tccgataaaa
      541 tttcgttact tttattgaca ccaaaaatac tcggtttttt gggatcggtt tttccgtgat
      601 aagacccacc tgctgtcata ggaggattat atcaaattat aagatacttt ggagataatc
      661 gaggactttt tgctgagtca agagaaagta aaccaaatta ggatcatttt taagctgggg
      721 atttttggta aaaatgtctt caacttcttt ttgttctact ttaatttctt tatcgatggc
      781 aatttgattg atggctaggt ttaaggccca atcttttttg atttgaattt ctaattcttc
      841 tcggtatttg ggtagagtaa tatttttgct ttttagatat tggtcgacag tcaatttggc
      901 tgactcgaga tgctcaacta ggtcggagag gcgacgattg acatcattat ttaataagac
      961 tgctgggagt tcgatttgag tgtgttttaa gattaggtca ataattttat cagttttaat
     1021 tttttgatca tttttttgat ctttatttag aggtttaatt tcgtcgaaga attgaggatt
     1081 aatgtttact ggaggaagct cggcactttc catttctaaa acccaatctg aatctaaaga
     1141 aagtttttcg gtttttattt taatggttgg ttcagtgatt ggctttaatt tgtgctcatc
     1201 gataatttct ttataagctt ttggcaaaag atgattgagg acttcttcaa taaccttctg
     1261 gggagaaata ttttgctcga ccacatcgag cggtgccttg cctttgcgaa aacccttttt
     1321 ttcaaaagaa gcctgaattt cggctaaaac atgctgatat tcttttttga tatcttcagc
     1381 ggctagggtt agttcaaggc taaatgattt atccttaagt tgctttaatt ggtgatttgt
     1441 tttcataagt ctgaatatga tagcataatt aaatattttt atggaaaaag taattgttaa
     1501 aatcgggcgt ttgttttttt ggggaatttt gggaggactc ttggtagcaa ttgccgtggg
     1561 tgagtgtatc tatttaatgg actctatcgg aaaaaatgaa tttatttatt ccaatttatc
     1621 aagtagcaaa tcactaacag tggcggctga atcaaatgat gtagaaaatt caggtgaaat
     1681 taaaagtcaa atcataaaag ctgatgctcg accggtaata atcgaaaaat atttagctaa
     1741 atataaatct ccccttttgc cttatgctag gcaaattttt gaaatatcgg aaacatatgg
     1801 ttttgattat tactggatag tagcaattgc ccagcaggaa tcaaatttat gcaaaaaaat
     1861 tcctgaggat tcttataatt gttgggggta tggaattcat aaaaacggga ctttaaaatt
     1921 tgaaagttat gatctagctt taaaatcatt tgctgaatat ttaaagagag aatattttga
     1981 taaaggcctt aacactccag agctgattat gaagaaatat tgtccccact cggatggttc
     2041 ctgggcacgg ggagtacagc agtttattga cgaattagaa gaaggtcgct gatacatatc
     2101 taatctatac tattgacata gttataatat tttggtatat ttccccccag atcccggggt
     2161 ggctcctcta aaaagcatgc tctgggattt ttttgtatca atctnnatct acaattaaag
     2221 tgctttttct tttgaagatt gaagtctgaa gatttaagat ttacaatacc ccctatggga
     2281 atgatttcaa cagcacagtt taaaaaagga atttatttac tttttcatga agagccacat
     2341 ctggtgatgg atataacttt tgtgtcacca ggaaagggat cagcttttta ccggacgaaa
     2401 ttaaaaaatt tatacaccgg tcgtgtagta gaatatacct ataaatctgg agaaaaagta
     2461 gaagaagtgc tcgtggagac acaggaggct gaatattcct attttgatgg taataattat
     2521 gtttttattg agccaagaac ttttgaacaa tacagtgtcc cctcggaaat aattgctgaa
     2581 gacaaaatat atttaaaaga agggcttctt tatagaatca aattctatga agataaaccg
     2641 gtgggaatta ctctgccaaa gagtattgct tgtgtagtcg tagaaacaga aaattcgata
     2701 aaaggtgata cggcaacttc agccatgaaa aatgcgattt tggatactgg agccaaagta
     2761 ctggtaccac tttttataaa aaatggtgaa gaaattttga tcagtaccga aagtggagct
     2821 tatttatcaa gaaaaaacta aagtccaatt tcaagatcaa taactggaat ttctatttcc
     2881 aagtcttggt ttatattatt ctcaatagtt tcaaattttt cctgataaat ttcaggtaaa
     2941 agtgatttag agacggtcgg agcaatgatt tcagttggag ttggagtgat aatgggagtt
     3001 attgaattgc tgttttttcg gcgagaagag acaattaaag aaataataaa aaggagcaaa
     3061 ataattgccc cgagagcaat caagagaatt attttagggc ttagtttttt ggaagtagca
     3121 ggaggagagg caggcatttc ttctcggaaa ccaagagggt tattttgggg aatatcgata
     3181 ttttgatagg gaatttcggc cattttagtt ataaaattta atcgttattg cttttaaatc
     3241 agctaaaaga ctttctagat atgaatctgc tttttgagtt tgaaccctaa cttcgggata
     3301 gtaattattg aaagttcttg ctttttgggg tttttgagca agggcggtgg cagtctcaag
     3361 tgaatttttt attaaatcag atttaacggg aaaattggca aaccaatctt gagtaaaatt
     3421 attatttttg gggtcagttt taatttcctc agctaataat ttgatttcat ttagattata
     3481 aagacgaaga ttaacatcgg cctgagttaa agcctgataa aaagcatttt gaatttcaag
     3541 atagcgattt ttgaaaagag tcgcccactc tttgatgtca ctagagttgg taaaattagg
     3601 aataatcaaa ttttgttcat caagccattc ctcccatttt gaaagttgaa tctgattttt
     3661 ttcggtattt acggaattaa tatttttata tttatcgaga ctgacccgaa gagccattaa
     3721 aaaactttta agcattttat ttctcgaaaa taagacggtt tttgtcgatt caaccatatc
     3781 ctgctcagag gtaatactgc gatattgaat atagacattt tttttgttca aaaaatctaa
     3841 ataatttttt tggtattcat ctcgtaaaaa tagataatct tgacgatatt gagtaaacaa
     3901 ctcatcctgg gtttgagcct ttacgggaga aagattaaag aaaaaaaata aaagaaaaaa
     3961 taaccacata aaatagttta gcagaaaaag tgccgctgag aggactcgaa cctccacatc
     4021 tttcgatata cgctcctaaa gcgtacgtgt ataccaattt caccacagcg gcaatttgtt
     4081 aaatatatgc tttatcacca cagtactggt aattattcaa taataccaat ttcacctgcc
     4141 cgcagttgct aaagcaacag cttttgcagg cgggccacag cggcgatatg cctattttat
     4201 caataatttg gtaaaataac gaagctgtcc ccatagctca gttggataga gcgtctcccc
     4261 tctaaggaga aggccgcagg ttcgatccct gctggggata caacaaagcg cctatagctc
     4321 aatggataga gtaatagtct tcggaactat tgatgagggt tcgattcccc ctgggcgcac
     4381 ttaacaactt gggtccttag ctcagcggta gagcagtcgc ctcttaagcg attggtcgcg
     4441 agtccgaatc tcgcagggcc cactagtatt aactcataaa ttcttttagc caggttttga
     4501 aacgggtcca gagattatct tttggttgat agatggtaga agtgggacta agagatttaa
     4561 tttcatactg aggagtatct ttccagccaa gataacttgt ttccaaataa tatttaatcg
     4621 taaaattaaa tggtattttg gctattttac cgggataaat atagttacta ccggtacaaa
     4681 tgggggtgct ttcaatatta tcagaagact gcggatttaa acaaaaatta gtctctcccc
     4741 agattgattg gccggtattt ttaagttcaa aagagccgtt ttgttcgttc tggctgaaaa
     4801 taattaaagg taagttaatt tttagaattt gaaactgagt agtttgctgg ggatgatttt
     4861 gctttttggg tagatccaca aatgattgat attccggata taattttcca tctggatcga
     4921 gccaggaaaa atgatcgaaa ggggcctgac tgtaattata agtaaaaggg gtgacagcga
     4981 ctactttttc gtctttctcc caaatttcaa aagctttttg taaaaaatca gcactagttt
     5041 taagggaata ataatcatta tttttggtga ctccctctcg atgaggccaa ccggtttcag
     5101 tgataaaaac aggataagtt ttatttacac caagatattt taggtaatca agctcccact
     5161 cataaccccg aatggacgtt tgaccattgt cctcaggaga gccgataaag ccatgattcg
     5221 gataggaatg cgaagccagt ccatcaatta aatcaaaata acgtgaatta tactgataaa
     5281 tttcttggta aaaatttttg gctgatttag tttggggcaa tttttcggga gcggctaaat
     5341 caaatggtga ggataagatg aaaaaatttg aatcataatt tttaaatttc tcggctgtgt
     5401 agataaaaat atcaacaaaa tttttaacat cgatactgtt cccccattct ttggcgtggt
     5461 taacctcgtt aaaaggaata atgtattttg gctgaactgg ccagtttagc gaagaaagaa
     5521 gggtggcaag gtgatcaata tcggcagggg tcggtatttt ccaggtggaa ttttccatga
     5581 tagtggcgag tctaataatt ggaataagat ggtatttacg gcaattatca aaaaaactct
     5641 gccaaatatt tttgtcaagc tggtcggttc taatgacgat ggtgatataa ccccagtccc
     5701 cgccgctcga attaattatt tctttggcgg agaagatatc ggcgctttgg gttaagtgaa
     5761 gaccaaagag gtttcgttca gatgctaaaa tagggttgat attcaggaag aataagatta
     5821 ataaacaaaa aataaacata gccccagtat tctactaaga aaatgaataa agaagaaata
     5881 ataaaaataa aaatctcccg agtggagaaa gaaattaggg atacccctta cgataaaagt
     5941 agcgaacatc atcacggagt cttaaaagct aaattatcaa aattaaaaga cgaacttgag
     6001 ggtccaagca ataaaggtgg tggtggaggt ctcggatatg cgataaaaca ttcaggtgac
     6061 gcttcgattg ttctcgtcgg actcccgagc gtcggtaaat caactttatt aaatgcggtt
     6121 accaacgctg aatcaaaggt gggaaattat gattttacta ccctcggtgt agttccggga
     6181 atgatggaat acaagggaat aagaatacaa attctcgatt tacccggaat tattgaagga
     6241 gcggccaggg gtaaaggttt tggtaaaaaa gttttatcgg tgattagggc atcaaatatg
     6301 gtggtgctta tgactgatat tcaaaggaaa gattggcttt ctaaaatcaa aaaggaactc
     6361 gaagaggccg gaattagatt aaataaaaag gcgcctaaag ttcatattca ccggatgaca
     6421 aaaggggcaa ttcaaattat tgatcctttt aattcgcttg ataacgaaac gatggtggaa
     6481 atagcgcagg agctaggttt tgagaatgct gtgattcagc tgggggaaaa attaaacacg
     6541 attgatcagt ttattgatgc cctcagcaaa acgaggacct atattcccct ggtagaagtg
     6601 attactaaaa tagatatgga gaaaaaatct aaagtcgaaa gtataaaatc taataacagt
     6661 gaagttattt tgatgagtgc tagtatggat attgggattg atgaatttaa agaaaaagtt
     6721 tggcagggcc taggactgat tagagtttac ttaaagagag aaagaaacgg tgaggcggat
     6781 aaaaaagagc ctttgattat gaaaaacgga cagtctttag atgcggtcct aaaaaaagtt
     6841 tcaggagaaa tgagagatga tgtaagccgg gcctatattt ggggtaaaaa tgcacgtttt
     6901 ccaggtcagg aagttagttt ttcttttcca gtttttgatg agatggaagt gtggtttggg
     6961 agataagatc ttgaagtttt tgggaacgct ccttaattat ctctttggaa attttagtag
     7021 attttaaaaa aagttcgcgg gctttggtgt ttgggcgggg agaatattta aacacatgaa
     7081 ttttggaaaa gccaattttt tggcaaagat tatatgtttc ctgaaaatcg atatctgttt
     7141 ccttaggaaa accaacgatg atgtcggtgc caaaagagag attagatttt agatttttga
     7201 ggctagaaaa tttttctaaa ataatttttt tggtgtaatt tcgtcccatt aatttgagga
     7261 ttttgtcgga gccggactga atggggatat ggaaaaaatt agaaacgcga tttgggaaac
     7321 gagaaactag atttataaaa ttttgattaa tacagttaat gggaatagag ccaaaactaa
     7381 ttaaaggaat tttggttttg gtcaataaag catcaattaa attagagaaa ccaggtacgt
     7441 attgatttaa attaactcca gtaattatta cctctttata gccatcggcg atggctttat
     7501 tgaccgtaga tatggcattt tcaattggta atgaccagag ataaggacgt ttttgaggta
     7561 cggtacaata agtgcaaaag gcagtacagc cggattgaat tttaagtaga tagcggtcag
     7621 tgtgagaaaa tttatctttt atttggtggc tgtaagtaga atctaaattt tttagataag
     7681 tttctttttg gagattattt aatatttgga tctttttgct taattttaga ttttttaaag
     7741 aggcacagcc ggagataatt atttttgcct tggggtattt tttttggagt cgacgaatta
     7801 ggccgagaga ttcggtgttg gcttttttgg tgacagaaca agtgttgata aaaataatgt
     7861 cggggttgtg gctacttggt aaatattttt tcttgattaa agactgagca aaaagattag
     7921 tttcagcggc gttgacccga cagccgagat tatagacagc aaaagttttc attatcggcg
     7981 aggattcttt gaaataaatt ctttaatata atcaagaata tcgatggtag tgttccaacc
     8041 aagctcttcc cgggcttttg tgggggcggt
//
LOCUS       DHGB01000010            3072 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_40547, whole genome shotgun sequence.
ACCESSION   DHGB01000010 DHGB01000000
VERSION     DHGB01000010.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_40547"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT          975 a          523 c          609 g          965 t
ORIGIN      
        1 tcgagacaag attgaactaa atcagtggga gtaaattttt tagactttaa accctttctg
       61 gcttctgata aagttaattt atttaaatca gtcatttatt tttatcgatt gtggcggtgg
      121 ttttaaaata gccactggaa actttttctg cctgagagag agctaattcc tgagataaag
      181 aagcttcgac tttgtcttca cgcaaaatat tacttttatg atttacctga aaggttggcg
      241 aaacgtcact ggtatctaat tcatctaata cttcaacgta tttcactgtc tcaattaatt
      301 gatttttaat gttcgtctca tctggcttga tttccagacg ggaaagcttg gctgtgtgct
      361 caaagacaga gtcagtaatt aaattatcag acatagtgta ttttaacgaa aaatggaggc
      421 tttgagacga agtagtttcc agcgatattt atcgacaagg ttgtatattt tgtagtaccg
      481 ggggttgatg accaaatcgt aggtgccaat gaactggaca aggttgccac caaagccttg
      541 tttaaaacga tgaaaaccaa aacctggtag accagcgtgg gcatcgggac cgaggcaacc
      601 ccacatatca aagcttttta aacctaaaga ctggccgtat ttgataactt cccacattaa
      661 taaagttgga gccatcactt cacggtggat gtcgagagag gccccataag gataaaaaag
      721 acggtctttt aatttaaaaa ccataaaagc tgccagggtt tgattttgat aagtggctaa
      781 aaatatttct gggatagttg tcttttttaa aattgaccac tgacgacggt ggtaattttc
      841 agagtgtaaa taaaatccct ggcgacgggt ggtatcaaaa agcattttta aataaatttc
      901 gaaatcctca tctttggtgg agtgagaaat agtaactcca tgccgattgg ctaatttgat
      961 attgtagcgg gttttgggtg acatatcgga aagtagctcg ttttcagatt tttttaaatc
     1021 cacaataaaa gaatggggat aaaaagcaac cttaggagaa gtaacgagag ctggataatt
     1081 taagacgatg cttttgtttt ttaataattg atttttgtta tcataaattt tttcaatctc
     1141 atctggttct attttgacaa aaatagcatt ttcacctttg gcaattttgc caacgttttc
     1201 aatcatatcc ttatcgattt tgggaccgcg gaggatagtg ccgatagaga aattggtatg
     1261 agggatttgg tgaaaggaaa gagtgtagcc ggaaattatt ttttgctgat caaaaatacc
     1321 aagacgatag actttatgcc cttctagttc ctgaaattca ccccactccc aagtctgaac
     1381 gggatgagtc acaacccgag aataagcttc cttttcttct tgatataaaa ttcgagtaat
     1441 cataatctca tattagtata tatcaggatc gtagactaaa tcagaattgg aatctctttt
     1501 ggtgaatcga tgaggagaac gaaaatcacg agtgatttcg gaaaaagagt catactccac
     1561 cagttctttg ggaatatcgt ataaaaagcg agaagcctca ttgagggatg atctgccaaa
     1621 aaaaagacga cgaatagcat aagtgatgta aagtaactcc ttggcccggg taataccaac
     1681 ataacagaga cggcgttctt cttcgatatc tttgtcgtta atcatcgatc gagaatgagg
     1741 taaaatcccc tcttcaaaac caaccagaaa aactgcctca aattccagac ccttgctact
     1801 atgaagagtc attaatcgga cgccattgcg attttctttt tttttatttt tttcctgaag
     1861 ggaatattcc tgctgaacta gggcaatatt ttccaaaaaa tcatttagtt taggataggt
     1921 ggcggcaact gattttaatt ccttaatatt ttcaattttt ttaagatcat cttcttcttg
     1981 gggattatat ttatttagat aaccggaatt aatcaccagt gattctaaaa tttgaagaga
     2041 ggataatttt ttcagactta aattatctaa atgctggtca aaaattattt tgcggcgttt
     2101 tcccaaggct ttttctaccc gggctcctga cagagcatct ttgggattcg aggcaacacg
     2161 aagcatggca ataatgtcct taatctcagc ccgatcataa aaacggagac caccaattaa
     2221 aacataagga atactatttc tgagaaaaga ttcttcaata gctcgggact gggcgttcat
     2281 gcgatataaa acggcaatat ctttgtgagc aaaatgcaga gaagtatgaa gctcttttat
     2341 tttttcagat aaataatcag cctcggcgat ctcagaatca gcctcaaaaa cttttatctt
     2401 ggcgggagaa ttttttttgg taaataaatt taataccgga tgactagtat tcttggaaat
     2461 gaccgaattg gcggcgtcta atatattttg agtcgagcga tagttctgtt ctaaattaat
     2521 tattttcgtt gtcggaaaat caattgaaaa agaagataga tttttgtaat ttgccccacg
     2581 ccagccgtaa attgactggg atgcgtcccc cacagcggta atttgctgat atttggtagc
     2641 taacaatttt gttaataaat attgaagctc attagtatcc tgatattcat caactaaaat
     2701 ataatggtac ttttgccaaa atttttctaa tatttcagaa tgttgttgaa ataactcaac
     2761 ggtcttaaaa agtaaatcgt taaaatcaag ggcctcgtat tgaagtaaac gagattgata
     2821 tttggcataa atttttgcgg ccatagattc ccagaatccg tgagcctgag attcgacaag
     2881 acgaggactt tgaaaagaat ttttggcact ttcaataaaa tagagaagag aggagggttt
     2941 aaattctttg gtcgttagtc ccatttcaga aagaatggag cgaactaaat tttcctgatc
     3001 atcactgtca taaataacaa aatcgggaga tagaccgatg tttttgccat agatacggag
     3061 tacccgacag cc
//
LOCUS       DHGB01000011            3843 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_27745, whole genome shotgun sequence.
ACCESSION   DHGB01000011 DHGB01000000
VERSION     DHGB01000011.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_27745"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1030 a          985 c          442 g         1386 t
ORIGIN      
        1 agcctttggc gaaggatatc cccagcttag ttactccaat tattgcccct ctcggtcctc
       61 ggagactctt gtgtgtcgtc gatgtaacaa tatgagcaaa atccaccggg ctcggatgca
      121 ccccacccac aactagtcca gcaatatgag aaatatcagc taccagccac gcccctacgc
      181 tttccgcaac ttctccaaat tttttccaat caagtatcca tggatacgcc gttgtccctg
      241 caaaaattaa ctttggtttt tccatcatta ccatttctct caatttttta taatcaatta
      301 ttccattttc atctacatca tactgaaccg aattaaaata ttttcccgaa aatgtaattt
      361 tgggatgacc atgggtcaag tgtcccccag ctgaaagttt catgccacaa attttatctc
      421 cgggttcgag taaagccatg agtaccgccg catttgccgg agaaccggaa tacgattgca
      481 catttaaatg ctcgactcca aataattttt tacctctttc aatggctaaa ttttcaatct
      541 catctacaat cttatttccc tgataatatc tttttcccgg atacccttcg gcatatttat
      601 tagtcaaacc acttgccaaa gcccttttta cattctctga cacaaagttc tcactcggaa
      661 tcagtctaat tgtatcattt tgatatattt cctcttgctc tattaaatca aatattttat
      721 ccatatgtaa ttttactcta tttttacaac atccctcttc tttgaatttc cgccttaatt
      781 ctctcaaaaa cctcttctct acttccactc ccatctaacc acgcacatgg ccaacccgaa
      841 ttatcaaaat accatcggta actctgttga cattttctca tcgtttcaac ctcatcgaaa
      901 atatcagcat cattcttatg atttactctt ttaaccgcct cttctggatc caaatcaatt
      961 atacatgtca agtcaggtct taaagttatt cctctattta cggttaaaaa ataatctctt
     1021 ttttccactg gtgcataact aaaagtcgaa gcttcatatc tatctcccaa cacataatca
     1081 aatacttcta acgcaggctt aataactctt acagtgtgat tcgctctgtc cgccacaaac
     1141 atcaactgca aggccactgg atccactcct tcttcttgct tttttatagt tctctcaatt
     1201 aattgaccaa ttggagtatc tctagtatgt tcacgcgtaa ctaaaacttt tttaccattt
     1261 ttcaccaacc actcttcaat cttttcaatc tgtgttccct ttccacatcc tccaatcccc
     1321 tcaaaaacaa taaatttccc tttattttct ttcattttat ccttttttca taatatggat
     1381 aaccattttt atcaacccca gatctctcaa aatcatcaaa aacaccgggt ataatctcct
     1441 taaaggtatt taaaacctcc accatcgtac ttctaatttc aaaatgggcc gcccttgccg
     1501 ttctcagctc aaaaacatgt ctccattctc gccaattggc cgtaaatacc tcctctgagt
     1561 ttagcccaag tggcaaaaat gatctggctt cttcaggaga ccaacctgct tttcgcaagg
     1621 atcgataata aatttccatc gtattcacca ttttctcgat tgatatttct ccgctcccgt
     1681 ccgtcaaatt tattctttct tctatttctt tgtatcttgg aaaaataaat ttcaattctg
     1741 aattatcaac atatcttgtc gactcctccg tcactgatgc aattctatgt cgatttaatt
     1801 cccgcatcat tccaattgaa ttatctaaaa attttactga gattgaagta tgttccaaaa
     1861 cactttcatg tcctctggaa attaacattt tggcaaattt cgtcgcactt tctaatgtca
     1921 cttcaccttt ttctgattga taactagttc ttccggctat ttctattttt aataattgtt
     1981 gccttaaact tttactgtca tctaaaatca aaaatttagg aatttgtcta attatttccg
     2041 ccatggctat ttttagctcc tcaaacacat tccagcaata gtcttatcta tatcaatatt
     2101 tttccttgcg gtttttcttc agtttttatt tcaattcaaa agtcaccgtc aggtttttat
     2161 agaccgtggt acttcctggc tctgttactg cccctccacc cattcctgaa tcaagctttg
     2221 catacattgg gtaaacattt ccattgtttc caccatcatt aacacttatt actttcccca
     2281 attttcttcc cgaagccgtg gcaatacttt ctgctttatc ttttgcatct tttatcgccg
     2341 catcgtataa acttttttct gccgtgttag tctcatccat tgaaaaatta ggtccataaa
     2401 cattattggc cccacttttt gccagtaaat ctgccaaatc agaagctcta tcaatatctc
     2461 tcaaaataat ctcaatcgta ttatttacct gccactgacc tgggttctgt cccccctttg
     2521 gctcctgata ataacttaaa ctttgcgttt taatatcagc ctcgtttatt ccgaaatctt
     2581 ttaccgattt gactaaatct tccattttag tgtttacttc tgttatcgcc gcatttttat
     2641 ccatattttg ggccatcact cccgccgaat aattagctag ctgatttttt tcctgacttc
     2701 tggcttctcc attgaccgtc accacctctg cctgttgcca gataattctt ccccagttaa
     2761 tcctgctcca gggaacaatc caaactaata ttaaaacgag aaccaccgtc ccaattattt
     2821 ttttcatatt agttaattat ataataaatg actttactta caaattggac atttatcccc
     2881 actataccag caaccacacc tggtacaata agttagcccc tctgtactac cttttctaaa
     2941 agttattcca tcgacatttt taataataat aattccctcg ctcgcaaagc gtccactgat
     3001 actccttcct ccacaaccac ctccgcttac caaaactgtc ttcagttggc caaataaata
     3061 attttgcatt ctcgatccat acaatgactc tgccaatcga ccaaccactg gttctacttc
     3121 tccccgcctt tccacctcaa gtctcgtcgc cacaaaaagt ctagtaatta actctgcatc
     3181 cacaaatatt ttttctccaa actctctatt aaatctttcc accaaggatt gagtaatttt
     3241 ttcaatagct gatattgaca ccctttcatc actttttgcc aaattcaaca ttttaatcaa
     3301 atctcccatt ttataatttt ctttatctat cttctcaaaa tctcgaagct gctccggact
     3361 catctccact ttaaatctca tcaaagttaa tttatctccc tcgcttcttt tccaaaaatc
     3421 aaccatatca tcaggataat caagctcttt atttttaggg ctaatagtta ccacaagttc
     3481 ctttcccttt tccaattctt cttctatctt attaatctta ttatgttcgt attcacaatt
     3541 atttgttaga cttcgtgctc ctattccagt atccctatca acaattttat tttcttgcca
     3601 taaataatca tgactatatt cactcaaaag tagctccgat agggctgtat tgatttctgt
     3661 ttctagttgc ctttttctta actcatctga aaactgtccc aaatagctta cttcttttac
     3721 tggatcaaaa tttagtcttt ctttattcat tttcatgctc gtgaattggg ctcgggctat
     3781 caccgtagcg gcactgcgag ggaatcacac cctacttcta taattatttt acacttcttc
     3841 ttt
//
LOCUS       DHGB01000012            4290 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_27744, whole genome shotgun sequence.
ACCESSION   DHGB01000012 DHGB01000000
VERSION     DHGB01000012.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_27744"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1391 a          603 c         1062 g         1232 t
ORIGIN      
        1 cttcatattt ttggtttttt cattgaattc attacagaaa tccatgattt tgataccctg
       61 aggaccgaga gcggtaccaa ctgggggagc aggattggcc ataccggctt gaatagctag
      121 cttaacaata gttttaattt ttttaggcat atttagatta ttagcttaat agattaatag
      181 attaataatt agtgaattag agttctttgg aaacttgtaa aaagtcgagt tcaactgggg
      241 tttcccgctc aaaaaaagaa acgagaacgc gaagtttacc tttttcctga tcgatggagt
      301 cgatagtgcc gataaaatca gcaaagggcc cattggttat tttaactacg tctccaagag
      361 aaaagggggt ttggaatttt ggtttgtctt caaccgtaat tttaataatg ttgtcaacgt
      421 ctttttgaga aataggagtt ggtttactgc caattccgac aaagccagtg acaccttgag
      481 tggtgcgaac ggtaacccag gaatcgtcgg taataatcat ttggattaaa acatagccgg
      541 gaaaggtttt ttcctgagtt tttactttgg tgccccggcg gataaccatt tttgattgga
      601 tggggacaat ggcctgaaaa atataatcat caagacccat ggttttgata cgggttttta
      661 gggcatcgat aactttgtat tcgtgacctg aataggtgtt gatgacatac caggccgcgt
      721 caataccggg ttctttttga gaaagagtga atcgaaattc tttttgttta gttttggtgg
      781 aattgtttgg catattattt acttattgtc ggctggagaa ggggattgat tgatggagca
      841 gggctcgggg ttatcacctg agtctgaggg ctattttttt gccccatgag cgccatgcca
      901 ttgatgaaaa ggtaatcaaa gcctcctaaa attaaagaga caataacaga aatggatatt
      961 accacaattg tcgactgaat tatagattca cgtttgggcc aggagatttg cttaaattca
     1021 gctttgacct ccttaaaaaa attaatgatt ttttgcatca gggtattata acaataaata
     1081 agaaattttt agttggaggt tattgactgt aaatttaccg taggtgacac acttgatatg
     1141 gttttgatta ttaaattcta gttgacaggc accaagggta gtgtgtatga tgagacacaa
     1201 acgctatgaa tagtgttatt aataaatagc gtaaaaatac atgctttgtc catattgtgc
     1261 ctttgatgaa tcaaatgttc ttgagtcccg agaagccttg gatggtaggg cgacaagaag
     1321 gagaagggaa tgcttgaaat gtaaaaaaag atttacgacc tacgaaaaag tagaaaatat
     1381 agaactgaaa gtagtgaaaa aagatggccg aattgaagat tatgagcgag aaaagctgga
     1441 aaaatgtttt gaaaaagctt gttggaaact aaacgcagaa gaaagatcaa ggttgattga
     1501 tgaaatcgaa atgaagcttc tcaactggga tagtgtggaa ataccctcgc gggagatagg
     1561 taaactagta atggataaac tgaaagatat tgatccggta gcttatgtcc gtttcgccac
     1621 tatatatttg gatataaaaa atattgaaga ttttcgaaaa ttattaaaag cgatggataa
     1681 ataaaatatg gatgaaataa atcagagaga taaaaaccct ctgtcctcca aatcggagga
     1741 catctccctt gaaaagggcg atttaatgga accggtgatg tctgagaatg cgaagtatat
     1801 tgccgagacg cgttatgcca tgaaggatga agaaggtaaa cgagttgaga gtgtcaaaga
     1861 tatattatgg cgagtagctc taaatattgc taggggtgat ctagtttttg ataagtccac
     1921 cttcgctgaa gctacggcgg attcgcaggc aaagatattt tataacctgc tcgcggagca
     1981 gaaatttttt ccaaatactc cctgtttagt caatgccggt aaagaaaaac agcaactatc
     2041 tgcctgtttt gttttgccca tcgaagattc aatggattca atattggaaa ctatgtccaa
     2101 tatggccaag attcataaaa gcggtggcgg gaccggtttt tctttttcaa gattgagacc
     2161 aagcggcgat tatattaaga gtagcggagg gactacagtg ggaccagtgt cttttttaca
     2221 ggcttataac gacgtcactt ctcaaataaa gcagggtgga gtgaggcggg gggcgaatat
     2281 ggggatgctt tcgattgatc atccggatgt cctacgcttt gcggtagcta agcttgatga
     2341 atttagctta actaatttca atctttctct cgcagtaact aattctttta tggatcaaat
     2401 tgaaagagat agatcttttg ttaaagagga aaattttccc gaggaaatta ttgaaaaaat
     2461 tagaaaagcg gaggctaacc gcgatgttga tgaaagactt cgacagattg aagaagaaat
     2521 taaaaaacta tacgaatggg ctttggccaa agaagaagga gaggggtatg aactaataaa
     2581 tcctcgagac ggaagagtgg ctaaaaaact gaatgcttat aaagtattta atttaattac
     2641 gagactggcc tggcaatatg gagatccggg actagttttt attgatcgga tgaacgagcc
     2701 aagctctaat ccagttccaa aattgggcag aattgaagca acgaatccct gtggtgaaca
     2761 acctctctta ccttatgatg cgtgtaattt gggtagtatt aatttaaata aatttgtgat
     2821 gaagggagat ttgaactggg atgaactagc cagggtggta aaagaagctg tccattttct
     2881 tgataatgtc gtcgaggtaa atgtttttcc ggtggaaaaa ataagagaaa tggtaaataa
     2941 gacgaggaga ataggtctcg gggtgatggg ttttgcagat atgttattta agcttggagt
     3001 tgcttatgac agtgaagatg gtctgatgtg ggccgagaga gtaatgaagt ttgtttctga
     3061 gtcagctaaa aaagcgagtc aggagctggc aaaagaaaga ggagtgtttc cggagtggga
     3121 aaacagtgtt tttgcaggaa ctgattatag acctagaaat atggctctaa cgacaattgc
     3181 tcctactggc actatttcca tgattgcgga tgcttcgtcg ggagttgaac ctctgttttc
     3241 attggggtat caaaaaaata cggttgaggg taaggccctt tttatcatga atcctatttt
     3301 tgttgaagag ttgaagaaaa ggaatttata cagtgaagag ctgatggata aagtagttaa
     3361 aaatggggga aagttggaag gtcttgatga aatcccccaa gatttaaaaa atatttttag
     3421 aacagctttg gaaattagtc cggaatggca tattaaagtt cagagcgcct ttcaaaaata
     3481 taccgataat gcggtaagta aaacgattaa ttttcctaaa ttggcaactg tcgatgaggt
     3541 tagaaatgcc tataaattgg catattctct gggaacaaaa ggaataacga tttatagaag
     3601 cggaagcagg gagaaagaag tgatacaaac agtgaaaggt gaaaccacgc caaaagcttc
     3661 gcaattaggc gggttagttg cagaaataaa agcaactaan naaggtttat acttcggtat
     3721 tttttgagga agggaatgga ccggtggaag tatttgtaac cctcgggaaa agtggtggtt
     3781 atttggctgg atcggccgaa gtgacgggga gactggcatc cctcgcttta aaatatggag
     3841 ctgagttaaa agaagtggcg gaagaaatgg tgggtatttc ctgtggtcaa agggtgggct
     3901 ttggaaatga gacagttttg tcaatgtttg atgcagtggg taaatcactt ttggaaattt
     3961 cccgaggaga acagcttgat ttgtttactg aggaaaagat gagacttgaa gtaccggaaa
     4021 ttaaagcaga aaagaaagaa aatattttag ctgatttagg aaaaaagata aatgaaggtg
     4081 aaaataagtt tgtatcttgt cctgattgtg ggagtccgtt gtatgcggaa gaaggatgtt
     4141 tcaagtgttc aaatacttat tgtggttata gtaaatgctc atgataacaa ctaaaaatgg
     4201 agatagggga accagtgagt actttggtaa aaaagttagt aagggtggaa agctactgga
     4261 ggcaatagga acattggatg aattgcaggc
//
LOCUS       DHGB01000013           12329 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_93607, whole genome shotgun sequence.
ACCESSION   DHGB01000013 DHGB01000000
VERSION     DHGB01000013.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12329)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_93607"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    7484..7667
                     /estimated_length=184
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3680 a         2210 c         2571 g         3684 t
ORIGIN      
        1 aagagatttg ggattggcag cataagtaag tttgtcgaga ttgataataa aatcatcggg
       61 atgctgagaa agccaatagt gaataaaatt gctgccgata aagccgcaac cacctgtaat
      121 taaaattttc atgatctcta aagattagta ctaggtgagg ctgagggggt gctggtcgct
      181 ttttctaggt cagtgccaat gataaaaatg aggtcataac tcgaggtggc agcgagctca
      241 gaaacggtgc tgtcagaaaa ttcggggatt ttctgaagga aataatcagt ggcaatgtct
      301 tttttggcct gaatctgatt gctagtttct ttcttggcac tattgccagt actaacattg
      361 gtaaaaccaa ggctagttag ttgggttttg atagtcgccg cctgaccatt aatatcagtg
      421 gcatttaaaa cctgaatttt aatagtcttg ctaatttcag ccggggttgg agttggagct
      481 tcggttacca cgggagagct gatttcctca gtttggggct gggaatcgcc agtaggggta
      541 tttatttcat tatttttatt gttttggttg agaataaacc agacaatagc tgaagtaatc
      601 aaaatagtaa ttaaaaaaac ggcgataatg gggagaatat tttttttatt gttttccata
      661 ggagcttgaa tttgagttga attaataacg gggactgttg gctttattat atcagttttt
      721 ggtttggaat taccaataaa gaatgaaaga acagggaggg gtaggtttga ggctttttga
      781 aaacactggg ctatttcact ctcattgtaa ttttcaccag gaaaatattg agtcggcttg
      841 ggattcgcaa aaaaagcttg tgaatagtta agtaattttt ttaactcagg taaagttttt
      901 ttaataattg aggttaaata aacatgaccc tgatttgaaa gaatagctaa ataatcgtgg
      961 gtattaatgg gataaaaaag aaaatgctgg ccactgtcaa aatgggcgta aagtttaacc
     1021 aaagcggagg aaagagtttc gtaatctaac actttgaggc ctaatttttc taaattggaa
     1081 gtcaaaagaa gaaatttttc ctgattaaaa atgcgggttc gaactagtgt ccgttcattg
     1141 tcaggctcaa gatcaaaggt gacagcttcg gacgatatct taaaatctac tgaatcttta
     1201 gctaaggtaa cgacttcatt tggatcaaga gtggttataa ccgaatcata aataaaggtt
     1261 ttagtaataa tataggtatc atctaaaata acgtaaacct gggaaatatt atttttctga
     1321 aagaaagtgg cgagattttg gagattatca gcagattgcg actgccagag gtcaatttca
     1381 aaagaaaggg tgttattgtc agttttatca aaataaattt cgagggagtc ttttttaggc
     1441 caaaggacaa cttttggttt cgagaataac gacattaaaa tatcttaaca cattaccatc
     1501 tgaggtgtgc aaatgccttg ttggcctcag ccattctttc aatttcctga cgtttggtaa
     1561 tagcagcccc ttcattattt aaggtagcca aaagctcagc ggctaatttc cccgaaaagg
     1621 tgtgaaattg tttgtttggc cgtttacgag cagaggtaac caaccatcta atcgccaaag
     1681 aatattgacg ggaaccacga actggggtgg gcacttgata aacagctccg ccaatacgac
     1741 gaggtcgaac ctcaactttg ggtttgagat tgtcaagggc ttgctctaaa gtggtgataa
     1801 tatcttcact tttactgtct tttttaaata tttcgagggc atcataaacc tgcttttggg
     1861 ccgcattctt attacctgca tacattaccc ggttgattaa cttagtaaca ataatactgt
     1921 tgtacttagt atcgggaaga acttctctaa ttttgctttg acctttgcgt gacataaaaa
     1981 ataaattaaa tcctaaaatc taaattctaa aattttttaa ttaaaaatta acatataata
     2041 ttaagccgaa gctgaggcgg attttttcgc accatattta ctgcgagatt gctgacgacc
     2101 agcaacagct ccagcgtcat atttaccgcg gataatatgg taacgaacac caggaagatc
     2161 ccgaacccga ccacctcgga tagaaataat tccgtgctcg gccaaattgt gcccaattcc
     2221 aggaatatag gcagtaacgg ttgctcgatt actgagacga actttggcta cttttctcaa
     2281 agccgaatga ggctttttgg gagtcatggt tttaacaaca gtgacaacac cttttttctg
     2341 aggattatag gtggcaacac tagtattttt aatactatta aaattatctc gcagcgctcc
     2401 agttcttaat ttaagacgac gaactttgcg acatttacta attagttgat tataagtagg
     2461 cataattatt taatttcctg gctttttccg acaggaataa gacgtccgat aataacattt
     2521 tcctttagac ctatcaagtt atcaacctca gccagcagcg aagcctcggt taaaactcca
     2581 gtagtttgtt ggaaagaagc agcggaaagc caggaaccag tagtcaaaga tgattggatc
     2641 agacctaaaa gtactttgtg tccctttgaa gctctgccat ctttaacatt ttctttggct
     2701 ttatcatttt cagcttcata gacaggtctc gtcactacct gaccataaag gaaattggta
     2761 tcaccaggat cttcaatttt tagcttatca ctcatttccc tgacaataac ttcaatatgt
     2821 ttatcgtgaa tactaatgcc ctgagattca taaaccccct gaatggagtt tattaggtag
     2881 cgctgagccg cttcaagacc ggaaacttca agaatttccc tgacatcgac cggaccagaa
     2941 caaagctggg ttccctggcc gactaaatcg ccatcaccga ctaataaagt aacattgctg
     3001 ggaagtaaat aattgactgt tttggcagca ttattcttgt ctttaccgct aagttttatt
     3061 tcgtagccat caatattttc tttaattttt accttgccat cgatttcagc taaaggtgaa
     3121 ggtaatttgg gagtcctaac ctcaaataat tcctgaacac gaggtaaacc ttgagtcacg
     3181 tcgactccca gaactccacc agtatgttta gttcgaagag ttagctgggt accaggttca
     3241 ccgacagact gagccgcaat gaccccgaca ggaacaccaa agccaacgag ctctcggctg
     3301 gataaatccc agccgtaaca gtgacgacaa aggccgattt tagatttaca ggttagcgga
     3361 gaacgaacat caacagtttt gacttcactc gcctcaatta ttccttgcaa atgattgtca
     3421 actaaatcac cttttttagc gatgattttc ttgcttttag gatcaatgac atcactacct
     3481 aaaacccgac cgagaagtcg acgaccaaag aatttttcgc ggcttttttc gttgcgatcg
     3541 atggtccaaa aacgggtcgt gccacagtca tcttcgcgag taatggcgac atgagaggta
     3601 tcaaccagac gacgagttaa ataaccggcg tcggcagtac gcagagcggt atcagccaaa
     3661 cctttgcgag tgccgcgggt accagtaata tattcaaaaa cagagagacc ttcacggaag
     3721 ttgctttttg taggcagctg gacaattttt ccagtaggat caaccatcag acctctcata
     3781 cccgaaagtt gcttgatttg atctttacta gcccgcttga taccagcggc ggaaacaatg
     3841 cggataggag aatcactgtc taaagtttcc caggtctttt cagcaatact ttcagtaatt
     3901 tccatccaga cactttgaga gagacgtttt ttctcttcat tactgataag acctatttta
     3961 taattatttt caatctcgac taccctttta tcagcggagg caataataga ttctttttcg
     4021 ggaagatagc ccatatctgg catagagaga gataatccag agagagtaaa tccagcaaaa
     4081 ccaatgtcct ttaaagaatc aattagttta acagttttta aacgactaga gatttcgagt
     4141 gacttaacaa ttaagtcatt taaggccttg gccccagcca tgggcagatt tataaactca
     4201 aattcttggg gtaagacaga attaaaccaa acccgaccat aagtagtttt ggcaagctta
     4261 ccatcgattt taacggcaac taattctctt aatccaattt tacctgattg ataggctaaa
     4321 attaattcat ccttgtctcc cataatcgat aaatctttaa agccctcttc ggtttctttg
     4381 gcaatgtctt cggaacggac agaagtaatg taataacaac caacggccat ttcctgggaa
     4441 ggaatagaaa ctggagcccc gtcagagggt ttaagtagat tccttgaagg taacattaat
     4501 tttctggctt ctctttgggc cgccgcagaa agaggtagat gaactcccat ttgatctcca
     4561 tcaaagtcgg cattgaaacc cttacagaca caagggtgaa gccggatggc cagaccatca
     4621 gttaagaccg gtttaaaagc ctgaattgag agtttatgaa gagtgggggc gcggtttaag
     4681 agaacgtaag tatttttagt gactttttcc aaaatatcgt aaacctcatt aatacggtga
     4741 tcaattaaat ttttggccga tttgatatta gccgctagac cgctatccat gagctctcgg
     4801 agaacaaagg gtcgatacat ttcaagggct atttcctttg gtaaacctac ttgatttagt
     4861 ttcaattctg ggccaaccac aatgacggaa cgaccagaat aatcgactct ttttccgagt
     4921 aagttacgtc gaaaacgacc ttttttgcct cgaagtaagt cagagagaga gcgaggtaaa
     4981 cgtttactac tttttcgttt atttttagaa gctttctgag catcgataag aatatcgacc
     5041 gattcttgaa gcattctttt ttcattacgt agaataattt caggagcgcc gagtttaatt
     5101 aattttttga gacgattatt acgattgatt agacgtcggt ataaatcgtt taaatcagaa
     5161 gtggcaaaac gaccgccagt aagttgaacc atgggacgca aatctggtgg aataactgga
     5221 acatattgta atatggtcca agccggacta atagaatttt cgatcatgcc atttaaaatt
     5281 ctgattttgt acatgatttt tttctttttg agtttactac tggttttagc tagttctttt
     5341 ttaagattct tgacagtttc gggtaaatcg aggtttttta agacttcaag taaagcctca
     5401 gcacccattt tagcctgata aaaaatatca gctttaaact gagctaaata aaattcttct
     5461 tcgtcagtaa gaacggaaag aacttccata ccttttatct tgtcttccaa aaatttgatc
     5521 aaagattcga tgcgattttt ctcactggca gccttgtttt caattttttg aacatcttgt
     5581 ttgagtcgaa cttcacgctc ttctttagca atcaaaagct ggtctttgtt ttttatttta
     5641 ttatcaagat cttttttctc tttggcggtt tgagctttta atttttcgat agtctctttt
     5701 gagtcgatat cgatttgact ttgacgtttt tcagaattta cttccagatt tactagggct
     5761 tctttctttt tgtcagcatc aatagcagta actaagtatt tagtaaaata gacaacagct
     5821 tcgagctctt tttggggaac atcgagaatc acactgagag gtgagggagt atttcggaga
     5881 taccataaat gagcaatggg agcggccaga gcaatgtgtc ccatgcgctc tcgacggact
     5941 ttacttgtag tcacctcaac cccacacttg tcgcaaataa cacctttaaa tcggattttt
     6001 ttgtatttac cacagtaaca ttcgtaatca cgggaaggac caaatatttt ttcacaaaaa
     6061 agaccatctt tttcgggacg ccaagagcga tagttaattg tttccggttt taagacttca
     6121 ccacttgacc aggataatat ttcctctgga gaggcgagct taatctcgat accagtaaaa
     6181 tcgagcatat ttttaaattt aaacattagt tagcctcctt taaatcatca acttttgtct
     6241 ctgtctgtcc ctcttcaacg acaggtttca taccgacagg agaaatatcg agagctagtc
     6301 ctgcgagttc tttggtcaaa accttaaatg actcggggat ggctggctca ggaatagcct
     6361 ggcctttgat aatggcttga aaggcatgag tccgaccttc aatatcatca gatttaatag
     6421 tcaacatttc ctgcaaaata tgagcggctc ggtgagcttc aagagcccaa acctccattt
     6481 ctccaagacg ctgacctccc attctggctt ttccaccaag aggctgctgg gtaaccagag
     6541 aataaggacc agtcgaacga gcatgaactt tgtcgtcaac catgtgaacc agctttagaa
     6601 tataacctac tccaacaacg acatcttgat cgaaagcatc gccagtacga ccatcgtata
     6661 atttagtttt tccggtcaca gtgagacctg cctcagtaag ttctttacca atttgagctt
     6721 cactatattt ttcaaaaaca gggacggcat attttttgtt taatttttgt ccagctgagc
     6781 ccaaaataac ctcaagcact tgaccaaggt tcatccgggc aataactgaa agaggagaca
     6841 tgacaaggtc aaccggagtt ccgtcagcca aacgtggcat gtcatattcg ggcataattc
     6901 ggcaaataac acctttattt ccgtgacgtc cagcaatttt atcaccctct tgaatacgac
     6961 ggagttgagc gacatagacc ttaacaactt tgttaacgcc aggatcaagc tcatcgccat
     7021 catccttgcc tagagtttcg attttgataa caactccccc ttcaccgttg gggacacgaa
     7081 gagaagtgtc tttaacttca cgggcttttt caccaaaaat agctcttaag agtctttctt
     7141 cggcggtaag ttctttttca cctcgaggtt ctacttttcc aactaaaata tcattaggac
     7201 cgacagtagc gccaatcatg acaataccat cttcggttaa tttagccagt tcggcttcag
     7261 agacattggg aatatcttta gttaattctt cagttccaag tttggtttcg accacatcgg
     7321 ctttgtattc aaaaatttga atatttgtta aaacatcctg tttaacgaga cgatcagaaa
     7381 ttaaaaaaga atcttcgtat ccgagaccgt caaccgaggc ataagcgacc agtaaatttc
     7441 gaccgatgga taattcacct tgactagtcg ccggaccatc aatnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc gtctgcatat
     7681 gagagcccat gagggcacga gtagcatcgt catgatcgat aaagggaatt agtgaggcag
     7741 aggcaccaac aatttcattt ggcaccatat caataaattt aacctggtca ataggacctt
     7801 cggaaaattc gccttgatag cgaataggaa cccaattttg tttgatgaca ttatcttcaa
     7861 ttttgatccc gagatgggta atttgatgat tatattcatc atcagcatcc atataaacta
     7921 catcatcgga aaaggtgtaa tttttgccaa ctttgacaat ttttcggaaa ggagcctcga
     7981 taaaaccata tttattgacc ttggcgtaaa gagccaggta agtgacaaga ccaatatttg
     8041 gaccttcagg agaacgaacg gcacagatac gaccatactg agaagaatga acgtcgcgga
     8101 tagaaaaggc ggctctttca cgggtaagac caccgacacc gaccacggtg acacggcgga
     8161 gtaaatcaag ctcggccaaa ggattggttt gatctaaaat ggcagaaagt tgattgcttc
     8221 taaaaaaagt attaagagaa gaaattaaag gctggggatt gatcatttga gaaggagtcg
     8281 gtttttcctt ggtggaaagc aaggacattc tctctttgac cattctttca aaacgggcca
     8341 tggcgggacg gagagcgacc tgggccacaa tttcgccaca gcgacgaaga cgacggttgg
     8401 ccagagaatc gatatcatca aaatgagtcg cttcaccttt ttgaagcttg attaaatgac
     8461 ggatagtagc gattaaatct tcttttttga tgacccaatt ttctttattt tcatcagaga
     8521 aattaaaaga gaatttttta ttgattttgt gacgaccaac ttgacctaat tcataggtgc
     8581 gaagatcaaa aaaacgatca ttgaaaaatt tttgagcgtt ctcgagaaca actggttcac
     8641 cgggacgaag cttgcgataa aattccaaat aagcttcttc ttgggttttg gtcgggtcag
     8701 ccataatcgt accattgata taatccccga attcactgac cagttgtttg tcagttaaag
     8761 ccgcggcagc gcgaagaata atggtagctg gtactttctt tttacggtca atacgggcaa
     8821 aaataacatc ctttttaccg ataaaaaatt caagccaaga gccgcgaaca ggacgaatct
     8881 ccgcattgca aagagtttta ccggtagcgg gatcaacttc actagtaaag taaacaccag
     8941 gagaacggac aagctgattg ataactcccc tttcgatacc attgataata aaggtggctt
     9001 cgggagtcat ggtgggaaga tttagaagaa aaacatcttc ttcccggacg agaccggttc
     9061 ttttgttggt taatttagcc ttggtataaa caggaacagt ataatttaaa cccttaagac
     9121 gggcaatttc tggagtcaag gtaagcttgt cataaaaaag ttctccaagc tctaaaagcc
     9181 agttgttgcc ggtatagtcg gaaatgggag aaatggcgcg gacggtttct tcaaggtcag
     9241 tttttagaaa tttttcccag gattcttttt gaacagctaa taaatctaaa gcaggaattt
     9301 gaggatattt tttaccccaa ttagttcttt ttgcggaagg catcgaattt tttataaaat
     9361 ttaagtgaag aatccgccaa aagctacgca tttagacgga caagcgagta atgtaccaac
     9421 cgctcgccaa atgtggagaa ttatatactt aatttatatg tagagcaagg gggagattta
     9481 ctgggtaaat ttaaaggtag agaggatttg agaaaattct tcctgaatag tacttgaaat
     9541 aggaatgttt ggatctccta atccgtatct taaaataatt aacttattat tatgaggtat
     9601 tattattttc aaacctcgtc ctgcaggatg cgaagtaatt tcttgtaaaa gaccgatttt
     9661 attatcaact aaatagtctt tttcattgta tattttgtaa caaccaggac aaagttcatc
     9721 actattactt tcttcagtta aactgattga atacattttt ggcgttaatt gattgttttg
     9781 attatcaata atactaattt ttggaagact agcgctgagt ggataattaa attggaatcc
     9841 attttcttta tcaatatatg ttttccagtc ggcggtgggg tcgataaatt taaaggtaga
     9901 gaggatttga ttgaattctg aatcaagaga agtattttga ggatatttga ttatattgat
     9961 tcttttgttg ttgtttatca aaaaataact tataccagaa cccattccag tccaatttag
    10021 ttttattgcc tctattccgg caatattaat tttactacgg ctccattgag gatcaaaatc
    10081 ctcggcttga gcctgttctt caaaaaatgc atctaaggaa tcatttactt ttttagactg
    10141 aatatatatt tccatcttta gttcccgaga gtttaatcca ggtccactat gatcttcggt
    10201 gagcggactc attatttgta tttgccagga tcccggtgta tttggatttg gatcatagct
    10261 attttcagaa taattatttg ggtacttata aagtaaagtc gactttttca ttttcatagg
    10321 ttttccagtc ggcggtgggg tcgggggtgg gtgaaattga ctcagtgaga atgggacttg
    10381 gggtgggaga agaaagagaa agttttttag aatttggaga aaagataatt aaaatagaag
    10441 taaagaatat ggcgcaaaca gaggctaaaa ttaggtttaa tttgttcata gactgggaat
    10501 aattgttgtc ttaggaatga ctgtaaccga cggagctttt gtcgctgaaa tcgtagctga
    10561 tttggttgga gtaccagaaa cagtggcgga ttgagtgggg gcgaggtcgc aggttttttc
    10621 ggtacaggaa atagttaaat cttcaccgca ggtacaagta ttgcagccat cagtagctga
    10681 aaatgtttga ccaatagagt atttttgatc attgagttcg caaaaggcag tggggataga
    10741 tgttggaatg ttatttgggg gaagttcccg actatcggta attgattttt gagaatttaa
    10801 aaaggaaatg acaactaaaa ctaaaacaat aatgaaaata attaaagaaa agaaaaagag
    10861 atatttaaaa aaattatttt gttttttagg aggaagagaa ggtatggatc cgccttcgtc
    10921 agttaacttc ggcgagacaa tgggagggat aatggcttca gtgactggag gaggattaat
    10981 cgcctcggga gccggaggag cttcaatttg aggaggtggt tcaggttggg gttcaggttg
    11041 aggttcaggc tgtgttttaa ccatctcttc ttcctctggg gtaagaatag gctcgggagg
    11101 attaaccggc tctaattctg gctcaggttt aattttctcc tcgggaattt ggtgattgtc
    11161 ttttgaacta atactttcag cgtatttgtc taaaatatct tgataactaa gatttgattg
    11221 attgtctgtc atgaaataat aatctcattt ttagaataag agtgcaataa tgcaataaat
    11281 tgaatggtat tgtatattta attatgataa atttatttat gtttaagaaa atattttttt
    11341 cttttttaat tggattttta tttttattca caggtaaaat agttttggct ggaggtttta
    11401 acttaaaatc aattgggggg gttgatacta gtggccgaca actgtcacag tggtggtaca
    11461 cgggctcaaa ccctgttttt atcggtgaag caggggcagg ggcttcagtg gaaattgata
    11521 ttgacgaaga aaaaggaaca gcggtagctg atgaatcagg aaactggagt tatagtgctt
    11581 cggctttgac tgacggtgat caccaagtag ttctagcaag caatggatct aatatcactt
    11641 ttaccctaac tactggggtt gataatgtaa actgggatgc agtcaatagc gatggcggaa
    11701 gtgaagcttt gcccgctgcc ggagttatct ggcccggact gatcgttcta ctaaccggag
    11761 ccggaggaat tgtgggtggt ggtaaaatga ttcgtgcctc aaaaaaataa gttataccta
    11821 aaaacatttg gctgtgctca aaacgaatct gattcggaga ggataaagac ctattatcaa
    11881 gaaagggggt atgagctcgt tgaaaactgg aaagaagctg atttggtggt catcaatagt
    11941 tgtgtgattc gagaatcagc cgaaaataga gtttatggac taatagataa gataagaaaa
    12001 gccgaatccg cctccgcaaa tgcttcggcg agacgaagaa ttattttgac aggatgtttg
    12061 gtgccgattt ctaaaaaaac gataaaggga gtggatgaaa tgctaccgat taaaaaaatc
    12121 agttttaaag tggagccgct aagagataag aaaagagcgg ccttgattac tatcagtagt
    12181 ggctgtaata actactgttc ttattgtatt gtgccttatg ctaggggaaa agagatatcg
    12241 cgtaaaatgg aaacaatttt aaaagaggtt gacggagcca ttaaaaaggg ttttgaggaa
    12301 gtcgtactca tagggcaaaa tgtaaattc
//
LOCUS       DHGB01000014            3996 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_62826, whole genome shotgun sequence.
ACCESSION   DHGB01000014 DHGB01000000
VERSION     DHGB01000014.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_62826"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1495 a          651 c          823 g         1025 t
ORIGIN      
        1 aaaaataata tttataataa atgctttaat agggtagaat ttagtaatgg aacaacatcc
       61 aattccacag cagattactt cctacgaatt caaactggta ggagatatga ctctgaaaca
      121 atttgggaaa gcggccgggg gaataattat cgccttgttg attaactcga gcaagctggt
      181 attttttgtt aaatggccat taatttttat atttgcggcg ggtggtctgg ctctggcctt
      241 tgtccccttt caagataggc cactggagac ctggctgatg gcttttatta aatcaattta
      301 ttcaccaaca atttatacct ataagaaaga aagtcccaaa aactggctag aaattgattt
      361 gagcaagcaa ctaaaaagtg acgaggaaga agaggtggtg gttccaataa aggaggataa
      421 gaaagttaaa gaatttatta actctttgcc gtcgattgga agagagaggg aggaaataaa
      481 agaagagatt tttgaaaaaa aagaggaaag tctaaagcct aaagaggaag agaaggaaaa
      541 agttgaggaa aatctaaagc ctaaagagga aatagaagag aaaaaagaag aagaggtaaa
      601 gccggtaaat ttggatttaa aaacagaaaa actgggggcg acaggaacag tggtgtttgg
      661 agaaattccc atgccggata ttcccgattt accaaattta gtggtgggaa tggttcttga
      721 tcttaaccat aaaattgttg agggggcaat tgttgaaatc caagacaagg atggtaatcc
      781 aagtcgggta ctgaaaacca acaagctggg acagtttaaa actctaaccc aactgagtaa
      841 tggtaaatat ttagtggtca cagaaaaaga aaatttagaa tttgaccgag tagaaataaa
      901 cctaacggga aagatcgttg agccaattaa aattctcgcc aaatagtaaa caaataggtt
      961 attattaaat agcatgccaa atgatcctct aaaaattgcc atcaagggaa gcacccaaaa
     1021 caatctgccg attgaagata ttgttgatgg aatcgttctg ctcaaagacg gttcggcttc
     1081 gatggtgctt caggtctctt cggtaaattt cgatttactg tcagagcggg aacagagtgc
     1141 cctggtgttt gcctacggag gaattttaaa ttcattgaat tttacaattc aaattgtaat
     1201 taactcaact accaaagatg taagtagtta tttaaataat ttagaagaag cgaaaaataa
     1261 acaaacaaat caaaaactaa aagagagaat tacttcttac aagaaatttg tggaagaaac
     1321 agtaaaaaga aatgacgttt tgtcaaaaac tttttatgtg gtaatccctt tttctagttt
     1381 agaactggga ataaaaaatg ctcaaaaaca ggcattttcg gtcttaaatc caattaaaaa
     1441 gaaggcagag ttgccttatt caaaagaata tattttggaa aaagccaaag cggctcttaa
     1501 gccaaaagag gatcatctgg tccgactttt ttcgagacta ggactggaaa ttaaagccct
     1561 aaacaccaaa gagttgattg aattatttta taaaacctat aatgaggaca tcgctaacaa
     1621 ccaaagatta caaaatgttg actacatcgc cgcagcgata acaggaaaac aatgaggaaa
     1681 aatctacaat cttcaatctt caatcttcaa tcttcaatct tcaattaaaa acctaaaaan
     1741 nctaaaaatt aaaataaaaa atgaatttac tagatattat aaagaaaaag aaaaacgttc
     1801 ctgaagcaga aacaaagatt gaggaaacaa aaaacttggg tgaggcaaag caatttgtgg
     1861 aaggactagt gtcaattaaa gatattattg ctccgtcagc aattgaagtt gattttaact
     1921 acctcagaat tgacaacaaa tattaccgaa cttgtttcgt cgccggatat ccaaggtttg
     1981 tgggactaaa ctggttgtca actttaatta attttgacgc gtctttaaaa atttcgatgt
     2041 ttatttatcc gaccgatggt aaggaaattc tagatgatct tcgacgaaaa attgccgaaa
     2101 tggaagccga attaagtacc gatattcaaa ggggtagaat tgtaaatccg gcaactcagg
     2161 caaaacttga agatgccttg actttacagg cagatctagt taagggagaa gaaagatttt
     2221 ttcaatttgg tttatacatg actttgtcgg ccaagaccaa agaagagctt gattcggcaa
     2281 ccaaaaattt agagtcggga ctgggagctc taatgatagt gtccaaaaaa gccagtttac
     2341 aaatggaaga gggctttatc acaaccttac ctatctgttc ggatagacta aatattacca
     2401 gaaacatgga tacaacttca ctcgccagta ccttcccttt tgtatcttca gatctctctg
     2461 atgacaaggg aattatgttt ggaattaatg aacacaatgg ttctctggta atttttgacc
     2521 gattttcaat gcccaactac aactcagtgg tttttgcctc ggctggagcc ggtaaatcct
     2581 acatgattaa actagaagca atgaggtcac taatgctcgg aaccgacatt attgttatcg
     2641 atccggaaaa tgaatataag aacctggcgg aatcggtggg aggacaatat attgcttttg
     2701 gttacggaga agcctcaaaa ataaacccct ttgatttgtc actggtaaca gaagagggtg
     2761 aaaatgccct caacgacaag attttgactt tacacaaaat gtttaaaata atgctcggga
     2821 ctatggatcc gattgaagaa tcaattttag ataagtcaat aatggaggct tataaagcta
     2881 aagggatatc gcctgatcca gaaacccaga atcgagaagc accactgatt gaagatttgt
     2941 acaaagcttt aattggagct gaagaagaaa aggcaaaaaa cttagcggca agattggaaa
     3001 aatacattaa aggttctttt gcgggaattt tcaatcaaaa aacaacagtt aatttaaata
     3061 atagttttgt cgtttttggc attcgcaatt tggaggatag tctgcgaccg gtagccatgc
     3121 atattgttct cgattatatc tggacgattg ttaaaaaaga gctaaaaaag agaattttgg
     3181 tggtggatga ggcctggtat ttaatgcaac atgacgattc ggcggcattt ctgagaggaa
     3241 ttattaaaag aggccgaaaa tattatctgg gagtaacgac aatcacccaa gatgtggacg
     3301 atttcttaaa tacaccctat ggaaaagaaa tagtgacaaa cagctcaatt caaatactgc
     3361 ttaaacaaca cagcgccgcc attgatctca ttggagagac attctaccta tctgaaggag
     3421 aaaaacaact actactctca gccgataaag gagagggaat cttttttgcg ggacaaaacc
     3481 acgtggcaat tcaggttatc gcctcagagg aagaacataa attaattact tctaatccgg
     3541 aggaaatact gcggcaaaaa attgccgaaa aacaagcagc gctggtgaag aaggaggaaa
     3601 aaatagaaga aagtctaaaa cctaaagagg aagaggtaaa agaggtggat aaaattatta
     3661 ctgagataaa gccacaagct ttatttaaac cagaggtcga ggaatcgcca gaagaggcgc
     3721 tgcaaaaaag aattgcccaa atggaaattg aagaaaataa aaaaacagag gaaggactta
     3781 gcaaaatagc caatacgaaa aacaaaggtg aagaaagcga aaatacaggt ggatctttac
     3841 caaaatatca agaattgttt aaatcggcac ccccacttcc ccctttacct aagtttgaag
     3901 ctccagcaaa accgctgttt attaaccagc aaaaagagcc aattatccca caaccgccac
     3961 agctgattaa aaaggcaaaa gcagaatttg ttaaaa
//
LOCUS       DHGB01000015            8467 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_80168, whole genome shotgun sequence.
ACCESSION   DHGB01000015 DHGB01000000
VERSION     DHGB01000015.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_80168"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         2497 a         1964 c         1205 g         2797 t
ORIGIN      
        1 aataaagcat taaaagcctc cttggtaagc atgtgaacct catcaataat aaaaattttc
       61 tttttaagcc gaattggtga caaataggcg ttctccttta atcctctgac atcatcaatt
      121 ccccggttag aagccgcatc aatttcgata atatccagac tatttccttt ttcaatttcc
      181 aaacaatttt cacactcccc acaagcctcg ccttttttca ttttcagaca atttactgct
      241 ttggcaataa tcctcgccgc cgaggttttt ccgctccctt ttggtccggc aaaaagcagg
      301 gattgaaaat cggtctcact agaaataata ctattaattt tatcagatac ttcagttaaa
      361 tctaaatccg ctactttttg gggccgatat ttcaaatgaa aaactgacat ctggctaatt
      421 taagcaaaaa tggctttatt taacaacacc acaaactaca tcactttaac agattttcga
      481 ctccatggag tagccattga tctaaaattt cgtttatatt tttcttaaaa tatcttgctt
      541 cgtattttaa tttctgcgta caaattacaa tatcccccag tctggtataa ccatctttat
      601 ccgccacttt tgacatggga aaacccaata cctgggggat ataatccatc tttcggtatt
      661 ttatatttaa ctttttcgcc ttaactctac ccacaaaata aatgcttaac tcggtatttt
      721 tctcatatcc tttatttact aaagctctat taactaattt actaattatc ttttcttcaa
      781 gtccccaatt ctttggatgt ttaatcaaaa ttatattttt catttttcca tttcttcttt
      841 cactattttc gacaccagat ctccgcttgc ttttccggca acttgtttca ttgtttctcc
      901 cattaccgct ccaaaatttt tacctttttc gataatcact actttcaccc tttccctgat
      961 ttcatcttct cccatttcct caggtaaata agattttaca atttcaattt catagtcctg
     1021 ttctttcact agatcctctc tacccgcctt taaaaacatt tctctagcct cctctttatt
     1081 tcggagctct tttcttagga cattgatttc ctcagcctct gttaaaccct cagggggtaa
     1141 ttgcagctct cttttatcga taagtgacac taaaaatctg agaacttcta ccattttttt
     1201 atcacctgac tttaaagcaa caattacctc ctgtttaatt tgatcacgcc acatatcttc
     1261 tcgatttttt agatctcttg cgacattttt ctcgccacaa aataattttc tttttggcat
     1321 aacgaccaat tgaaggctta gcataagcca ctctttttcg aacttcttcg gtaatatctt
     1381 cctctagaca aagtctacgg aatttaccaa tgatatcgtc tttggattct ccttttttgc
     1441 gtttgacaac cattgccatt agctcacctc cttaatacaa gccctatttt acccgttatt
     1501 tattatcctg taaacactta atatagtcga gactattcac cactccatct ttattataat
     1561 cagttgtttt tgaacaatta ctcgcttttg ttggagtaac cattgtttgt gttgttttag
     1621 gattattttt caaacaatta acataatcaa gactattaac cactccgtcc ttatcataat
     1681 ccgtcgtctt attgcacgac atcaccccac ttccactggc cacttttacc gtcggtaaaa
     1741 ccgctactgt cggtacaact gcaatagttg gtatttgagt aggctcgatt gccaccgtca
     1801 tggttttaaa agaataagga atgccattat tatcaaaaac ttcctccccc accctaattc
     1861 taaaatagta gtttgtcccc gcttttagtg gacttaaaac cactgaatga ctggttgact
     1921 gactactttc aggcgctcgc agtaacaaag aagctggagt cgttccatat tcaactactc
     1981 ccatggtttc cttatcagtc gtccaagtga tagttgcact tttaccgtcc acgttacctt
     2041 ttcccaaaac atttttggga gctaaatcag ctgaagcacc acttagataa gttctagctg
     2101 tattgacacc aagcactcca agtacgccca ccaaaagcaa tatcactata attattaccg
     2161 tttttagaga aaacttacct ttcataaaaa tttcaaagca agcgaagata acacgctcac
     2221 aattccaaaa atcatcaaac cgattgtcgt ttctaccgtt cctgtttttg gtaattcagt
     2281 accagatgtt ggtatcgccg tcgggatcgc cgttggatta ggatccgaaa tccctccgtc
     2341 ggtaatcgta taaactccat taatattaga tggacaacta atcatatcag cagttgttgt
     2401 cccatcaaaa atatttgagt cggcatctga accagcagta catttaaaat caatgtttat
     2461 tgtcccagca accttaggtt taaattttat cttaagcaaa tcacctgaca cagtcgcttt
     2521 ctcttcaggg tgcttctcaa gacttatata gtaactgtca aaaaatgttc cgctcacatt
     2581 atcggtagtc ggaccaatcg acactaagcc aagcgtatca aacaaattaa ttactggctc
     2641 tgccgattga atttctactt ttgagggatc aaactttaaa tgaacatcta tggccatcgt
     2701 ttggacagca ccagaatcaa ccgctaccac cacctcaaag gcactatctt ttgtatatga
     2761 gccactactt ggtaccagtt tcatcatcgg ctctaccgcc caaacgggct tgactgccag
     2821 agctaaaaat aaaacaacta gtagacaaaa aaaatttttc attctaaaga accattaatc
     2881 attaaatttt cacttttata aggtaatcct tttattgtat tgttgtcaac tatgttagtt
     2941 tcttcatcaa taaataattc agctccagtc gttatttcag atgaaatgtc aagctcggcg
     3001 atttttacac tttggccagg atatattttg aaaccatcgt cttcggaaat cagatagttt
     3061 aaaactacca aagaattatc attgcttacc ttttgaaaag ctggttcagg tagttcacct
     3121 aaattgctta ccccaacaac ttcaatattt ttatatccaa tatataaatc taaagctgca
     3181 attgagactt cttcactcgc tgtcatcaat aaaattggtt tttcaccttt ctgccagcta
     3241 aaagaaacta tctcctcttt tgattgagtg tcaaccattc ctccgtcaat atcagtaatt
     3301 tttttttgat tagtaatcga aaataaactt acagctatca ccaccaaact caataaaaca
     3361 acaggtaaaa aatatttatt tttcatttta ttattctcca tatttacttt tttgttaata
     3421 tttactaata taattctcct tgcttttctt caagagtttt tctaagttga taaagatcgt
     3481 ttgaatttac ctgacagcta ccatcaagat cagactctat atagccacca tcctcaacag
     3541 tttctaaaac tttagccatt cttttcacct ccgcccagtc ataaccatcc acaactccgt
     3601 caggactatc agaatttttt ccaatcacat cacccggcaa aatcggataa acggtaaaat
     3661 cgtaaatcgg gctagtgtca attgatcttg tcaaagttaa ttctcctcca gcctttccat
     3721 aacgacttgc ttgcttatca actgcatact tcatttgtaa atgttttggt cccttcataa
     3781 acaccgctac attatcagtc ctatcaaaac cttcaagagt taaagaacct tgaacagtta
     3841 caaaatcact acttttatca actttacttg gaacaacgtt gttataagct tttgttactc
     3901 ctccacttaa cacaattact tgtagcggcc aatctttggg tacaaaacat tgactataat
     3961 cactttttac aaaagcatat tgaactttgt aattcaacac tggccactga cctgaactta
     4021 aatgacagtc ttctttgtaa gccctatata ttgtttgtgt actacttgga tcatcatcac
     4081 aagttttatc tgcctctctt aaccaccagg tatattgctg atcatttact ttactacatc
     4141 tcgaacactg attgggtttn ncatacattt tgcatcataa ataattgtta cctttgtatc
     4201 agcactagat gatgaaaatt tttctgaaaa atctattaaa ggatctttag aacaatcttc
     4261 attctgctga gcttgccagg ctaacctcat atggtcaaaa tagatactac atcgatcaca
     4321 atttttttct agcatcggtc gacaatattt tggaaggatt acatctaaac aattatcgtc
     4381 aggagaacag gttggctgat aacactcata gcctgaaggg cagtcagcat cagtctcaca
     4441 agtttgagag gatccaataa ttttataagt cgtattttca accgaagtca cagctatttc
     4501 tttatcagtc gcacttgcag gattatctcc agttaccatt gaagactctt tattaattgt
     4561 aatcgtacct tctccctcac tgagtgcgat aaaattcaaa gcaaaaactt caccggttgt
     4621 tttaagttta tcagccgcct gttgtgaagt cgctaccaaa gtcgcacagt tctctttaat
     4681 cactactatt gggctagaat taaaaccatt tgccgtatta accaccactc caccctcatc
     4741 taagcttaaa tgacttcccc aacaaacctc ggtttgcacc ccatcaactt tagcttcgct
     4801 ttcagtataa taataaacct tgacaggaac tatctcgtca attcttctct ctattgtagt
     4861 gttaagaatt aatttaagta ctgtgttcgc aaaactagca ccctttctcg tatcttgatt
     4921 ttgttttacc agcaccaatc ctccaaaaag tgtcaccatc aataaaatta gtagcggtat
     4981 ggttgttttt ttcatattat tttataaact tttgataaaa ttatccctcc agatagaaaa
     5041 atcattaaga ttaactttac cgttacagtc aaaatctgct ctccaactat tcttaaccgt
     5101 aacccccaga gcaccagtta taaattcacc tctccagatc gaaaagtcat ttagattagt
     5161 taccccagaa caatcagcgt ctcccttaga tttagcctca ggtttaccgg cacattgagt
     5221 acatgttgtc ggacatttac tcatatcagt tgtccaggat ccaccggttg tcggacagca
     5281 atagtttgtc ccaccgcaga cttcttttct cccggtgcta ttacatagtt tctcatccca
     5341 atcccattga ttattaatgc actgagcgtt aataaacagt gtctttgact cgcatttatc
     5401 ggcaggttta gacccagaac aaccaccagg aggattaggc gggttcgttc ctgttgtagg
     5461 tgtcggagaa ggcccgctag aaatataaca gtgaccattc caacatacat tcccagaaca
     5521 acaatcacct tgagaattac aagctccacc agtctttgaa catgtcttag ttggggttgc
     5581 ggttggttgt ctaggtgttg gaatatattt acaagaatca acactacaat acctattgta
     5641 accaccaccc gaagccgatt gccagttaca ttcgtcatta gcacaattat caacacaata
     5701 gcatttcaaa ggaccactgc ttgttggagt tgccgtcggt ttttcagttg gagtggcagt
     5761 cggagtattt gttggtcctc caggacaaga tgaatcatat ttgcaataat aagaacctga
     5821 aagcatacat ctatagccaa caccacaccc acctaaacta ccacactgat aaagtttact
     5881 tgctgaacat cctgataatg ttggaattgc agtcgcaggt ggagatatag ttgtatcagg
     5941 aatgcactca aaagctccat aacctccacc agaacatttt agaccactac aacaagattc
     6001 tgtagtacaa ggatccccca accctaaaca agttgttgga gtaggacagg cattatgaac
     6061 acacggacct gaacatgttt tagtttttac ccagacatta ttggtacagg tatattctgc
     6121 tgaattaaca catttccttg tttcatagac tgaattacaa gcgttaggat ttggagttgg
     6181 ggttggtggt ctcttggtcg gagttgcagt tggaggtctt ggagtattgg ttggatttac
     6241 acacaaagca ttactacacg aaaagctaca tgtttcaact gtataccata tattattaag
     6301 acatcgctca atatttttac cgttacatct tctcgtataa tttgtacaag taggagccgg
     6361 agttgaggtt ggtcttttgg ttggagtcgg cgtaactgct aacttacata aataattttg
     6421 acaaatataa ccagtacaac aagcagaatt tgacgcacaa tactgattta aacatcccgc
     6481 agcttgattt ctcgtatcct gactctcttg aactagcttt gtcgctactg gtaccaccac
     6541 cgccataatt aaaaggccaa tcattaaagg aatttgagct aaaccttttc tttttatcat
     6601 tttttaaatc tttatataaa gtattactaa ttaacagttt cacacattct gaaatcgccg
     6661 tcaagacgtc aattaattat tactccttga tataattttt tatgtctctt ttttcccaaa
     6721 aaaatttatc atttcttttt cttccctttc tttttattct tatctatttc ttacccaaaa
     6781 tttacctaga cttacgcaaa tctgcctctg gtactctcgc tagcattgtt atcgacactc
     6841 aaaatcctgg ttcaaattta tcacccgctc tttggcaaaa tctcgctcaa ggnncaaggt
     6901 ggcgaagaac cagtagatat gattaaacct gtcattaatc aattaaaact gcttaaaccc
     6961 caactaatcc gtgttgatca tctttttgat tattatcagg tcgacaaagg caatgggctc
     7021 tatgatttct cccgtcttga cggtgttatt gacagtatta tcaaaactgg cgccactccc
     7081 tttctctctc tttcctatac tcctgactca aaagagcccg ctgattggaa tcagtggaac
     7141 aatatggtca aagccaccgc tcatcgctac tctatcgaaa aaaatatctc tggcatttat
     7201 tatgaagttt ggaatgaacc cgacctcttt ggtggctggc actactccaa atcacccaac
     7261 tactcaactc tttatatcaa cactgccagg gcagtcgccg agggtgcctc aggtactatt
     7321 tataaaattg gcggtcccgc caccactggc tactatacta attgggtcaa atctcttttt
     7381 gccactgcct ctaaaaacaa tgttcctctc gattttattt cctggcacaa atattcaaaa
     7441 aatatcgatg actacttaaa ggattttgat gatttaaaca aaattctctc tgattatccg
     7501 cagtatttta atatagaacg cctcatcacc gaaatcggtc ccaattcaga accagactct
     7561 tggtatgaca ataagctcgg tggtattcat ttaatttcac tttgtaccga gctttcagga
     7621 aaaattcatc gtctttttac ttttgaagcc attgacggtc cctctcctcg ttctgacaaa
     7681 tccagtggtt ggggtatttt gactcatgat ggtaaaacta aaccccgtta ccatgctcta
     7741 ctctttttaa atcaaatttc cggcccccgc cttccttcaa aaggagatgg ctcttgggtt
     7801 acttccatgt ccacaaaaag tgcttctact atccaaactt taatcgtcaa ttacgatcca
     7861 aaattaactc atagcgaaac tacccctatc acctatcaag gtttaactcc aggtcaatat
     7921 cagttaaaaa tttctcaata tcaaggttca ctaagaaata aaactattac cgtaaactct
     7981 ccctcttaca ccgaaaatat atatttagaa ccaaacaccg cctatctttt agaattaaaa
     8041 cctattttat aaatattctt ctcacatccc ctatcgtcac tagcagtatt aatcccagca
     8101 gcaccatcat tcccaaatta ttaactaagg cttcaaattt tttattggcc ttctttttaa
     8161 ataaaagttc ataaaataca aatattattc tgcctccatc aagagccgga aaaggtaaaa
     8221 cattcacaat tgccaaattc accgacacta ctccaaaaaa atgaaccatc gctagaaacc
     8281 cttgattcat tcgaatacta ctcgtggctt catacatccc aagcggcccc gagacatctt
     8341 ttggcacctg acctaccatc aactcagcaa tcattttata aactccttct cctattattt
     8401 ttccccaaaa ataggcttct ttaaaccctg ctccaatccc cctataaaac tgataccact
     8461 ttatttt
//
LOCUS       DHGB01000016            3389 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_122792, whole genome shotgun sequence.
ACCESSION   DHGB01000016 DHGB01000000
VERSION     DHGB01000016.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_122792"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    1454..1500
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          884 a          642 c          524 g         1290 t
ORIGIN      
        1 aacttcgctg gcgtgatgat ctcactcaaa aatttatcaa atttgccatt gttggtggca
       61 tcggttttat tgtcaacata gttggtggtc ggctttttaa gaatttacta accccattaa
      121 tattatctgt ctctcttctc aatggagttg ccaatgccct ggctgcggag ttatccataa
      181 tttctaattt catctggaac aatctctgga ctttttccaa agaaaaaatt accggtagcg
      241 gcaaaattat tcccaaattt ttggctttca acgcctcttc cgctatcact ggtattgcca
      301 ttccttcttt ggttatcggc ttgttgacta cccttttcgg tgatcatttt ttggcttatc
      361 aagttatcgc catctttgct ctaaccattc ccttaaactg gttcgtttat aataaaatta
      421 tttggaaaaa gaaataaaaa tatgaaaggc attattttag ctggtggtcg tgctactcnn
      481 ggctccgtcc gttaacttta gttaccagta aacaattact gccggtttat gaccagccga
      541 tgatttatta tccgatcaat actcttattc agggaggaat taaagatatt ttaattatta
      601 ttgctcccga ttattccggt caatttttaa atcttcttgg tgacggctct gaatttggag
      661 ttcattttaa ttatgctatc caaaaagagc cgaagggcct tgccgatgcc tttattgtcg
      721 gtaaaaattt tatcgatggt gacaatgtta ctatgatttt aggtgacaac atttttgatc
      781 ctggtttcgg ggctcaaatt aaatcttttg aaaaaggggc tatggttttt gccaaaaaag
      841 ttgaagatcc ggagcgtttc ggtgtggttg aatttgacaa ggacatgaag gcggttagta
      901 tcgaagaaaa acctgaaaat cctaaaagta acttcgccgt ggtcggtctt tattgttacg
      961 actgccgggt tacagaattt gcttcaaagc tcgctccttc actccgcggt gaaattgaaa
     1021 ttaccgattt aaataatatt tatcttaaaa atggagaaat gaaagtcaat gtttttgata
     1081 gtctctggga agatgccggt acttttgatt ctcttttaaa agttagtaat gaaatggcca
     1141 ataatattgc taaattgaca gagtcagata aataagtata aaatgctgtt atgactaaaa
     1201 ataaatatct aactcaaatt gaaaagactt tagacctata tttcggtaaa aaagccccag
     1261 ccatgcctga aaacattaag gagttaattg taaaatatag tccatatttg accgccattg
     1321 ctttaatttt ttcgattccg actttattat ttgctctcgg ggtaagtagg gctttcatgc
     1381 cccttgcttt tgccggtgct tatcgtcccg gtctgtctat ttctataatc atttctttag
     1441 ttgccatgat attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 gctatttctt tttaccttct tttccaaatc aagtcttact ataaaaacta aatttccagt
     1561 aggctaagat tgttaattga tccgccttcc atccaattat tgccgtgaat tactagcagt
     1621 ttctgacctt ttttaataaa tttttgggcc aacagctctt caaatatttt attatcactc
     1681 ccatatttat gttgatcaaa aacccgataa aaggggatta ttccataaga aaaattgagc
     1741 atctgaaaag ttctctcttc atcggtgatg gcaatgatag gcattttgag acggtggttg
     1801 gcgagttttc gagccgatga ccctgatttg gtaaaaacaa ttatcccatc ggcactttcg
     1861 gatactttta ccgctgcttc cagcagggaa tcggcaaagc ttatactttc attttcaata
     1921 gtttttattt ttccattttc ctcactaaat ctggctattt tagccattac ttttactgat
     1981 tctagagggt atttgccaat ggctgtttcc tctgaaagca ttatggcatc actcccgtca
     2041 aagacggcat gagccacatc gctcgcctca gctctagtgg gagtatccct ttccgtcatc
     2101 gacagcagca tttcagtggc tactatcact ggtttatttt gttttctcga ttcgtcaatt
     2161 attttttcct gaaaaaaagc cagttcttca attggagcac ttctccccag atcccctcta
     2221 gctaccatta ctccgtcagc cgcttttacg atttcttcca aattcttaat tgccatcaag
     2281 ttttctattt tggcgattat gggtttaact atattttttc tttttaaacg ttcttttaat
     2341 ttttcaattt cctgagcgtt ttttaaatat gacagggcga ttgcatcggc ctcttggagt
     2401 atttcaaaat cacttctggg caggtcaata aaaaccggaa tcttgttatc tccaatttta
     2461 tggataattt taattatgtt ctcgtgccat tttttttcat tatattttag atttaaccta
     2521 aaaatattta ctccgttttc aatcagttct gataaaattt cctcggtttc actggctggc
     2581 cctacggtgg cgatgatttt tgttttcctt attgacacta tttaatcata ccagtatttt
     2641 tacttttcct gttacaattt ctttatggag actcttaaaa aatatatcta ccaccctgaa
     2701 attgacttta ttagaattat ttgtactttt tcggtaatca tgattcatat caccaccaaa
     2761 attttagccg tctccggaaa tgatttgttc ggtcatcctt ttaccttatt tttaaatcaa
     2821 ttatccagtt ttgccgtccc catttttttt atgatttctg cttttggctt aaaacttaat
     2881 tattctacta atcttaaatt tttcccctat ctaaaacagc gtttatctaa aattttgtta
     2941 ccctatctat tttggagttt tatatattat tattttgttt atccccataa ttctcagtca
     3001 ttttttaggg tattacttct cggtggtgcc tcttatcagc tttattttat tcccactttg
     3061 tttatttttt acttattatt cccttttatt tatcattatt ttaaatttat aagtcgtcct
     3121 cttcctctta ttattttatt tattatccag tttatttttc tctcgatcga ttattactac
     3181 cgccccttat cctttgctta tcccataaaa gtttttatct taaatttcat cttttttatt
     3241 ctggggatgc tagctgggga gtatcaaagt gaaatatcta attatgtaaa agcacaccaa
     3301 aaaaaaatta ttttcctttc acttatattt gccctgtata tcacttttga aagtgctttt
     3361 cgttatttta aaactcaaaa ctatcttag
//
LOCUS       DHGB01000017            5831 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_35302, whole genome shotgun sequence.
ACCESSION   DHGB01000017 DHGB01000000
VERSION     DHGB01000017.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_35302"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1683 a         1184 c          842 g         2122 t
ORIGIN      
        1 caaattattg aaactctagg tgatttttgc tataatctct tacttcgtgg agatgtaatg
       61 aattgacgga gttgttcttt aaaaaactgc aagccgactt tgattcatga gtcgaaaaac
      121 agagtcaaac aaaacatgcc aaaaaaactt ttggtgttga tttaaccaag gttgcaaata
      181 acgttaactc gttttttgcc cccaaggtca atgcttacgc tttagcttaa gcattcaacc
      241 attgtttatt ttcctattct aaggggattt aaacagtgtt aattaaatta gaatgctatt
      301 tttagaatct cgattttcta aaaatctaag aggggagcaa tgtccccgcc aatcgacatt
      361 ttaagaaaat tgataatttt tttcttaaaa attaagtcta aattattaaa gcttgtagac
      421 gtttttttta gtcagctttc ggacctgggt tcactcccca gcatctcctc cttcgcctcg
      481 ctgaagtccc aataaattgg gacgaaagcg ggcttcgccc taattcagct aaggcttcgg
      541 cgagacacag tcctcaaaat ttggtaatat ttctctcatg tattacgtct atattttaag
      601 ttgttgcgat agtggttatt atgtaggttg cacagataat ttaaaatcaa ggtttgaaaa
      661 acattctcgc ggatacgtaa gtgcgacaaa atatcgttta cctgtaacaa tagaaatgta
      721 tttctgtttt agggacaaat atcttgcttt taattttgaa aaatatctaa aaagcggttc
      781 tggtagggca tttaccaaaa aacattttaa tatataagtt atcttacccc tctttttatc
      841 tcttcttttt tgatcttctc tcttttttca aatttcctca tctttcttcc aatcccaatt
      901 tccatcttaa accaaattcc ctgtaaaaaa atcgaaatag gaatcatcat atatttttga
      961 tttcgataag attgcagttt ggcaatttcc ctggaattta agagtaactt tctttctcca
     1021 cttttatctg tttcctgatt ttgactatat ttataaggcg caatttccag atttagtacc
     1081 atcggaacac cgttgataat ttccactttg gcacccttaa attgaacccc gttaactcgg
     1141 agggccttgg cgtcagcccc agttaggacc atccctgcca ccatcttgtc actaaagtta
     1201 tagtcaagtg aatctttatt aaaatatttc attttatttt tattcgatag atttttccat
     1261 cggcggcgag gaccaacacg tctcgatttt ggttatctat cgcaatgtca ttaattatca
     1321 tttttccaaa ataatttttt cctagaattc taccatcttt atggtaaata taaactgtat
     1381 tattgtcggc gatatatgcc aaaaaatcta cctctgcgtt tgtttccagt gctctacttg
     1441 ttgttaagct cggaacaaag ctcatttcgt attttttctc ttctccccga atataatgat
     1501 agattcttcc cgacttaccc agaacccaaa tatctccatc aattgccatt cccgttgttt
     1561 cttccattaa acttattcct tcttttagcc atactattcc atttttatta ctaagctttt
     1621 caattttttg gttgtcatta tttaaaacat aaaagagacc attccagcca ccaatatcac
     1681 caatattttt aatctcttta atctcggtct ctattagact atttatcttt atttcataaa
     1741 ttttattttg gtcaaagccg tagcgtccat ctccattgtt aaatgttccc agccacaact
     1801 taattctttc atcgctgatc accttttcgg ttgattgatt tttaatatcg acgcggttta
     1861 tttggccaag ctcactactc cacagataag ccaaatctcc tctgattgtc atgcctttaa
     1921 attttccgcc ttcaacaatc aagttagtgt cataggccac ttcataatcg atgctctctg
     1981 cccctaggct ctttttaatt ccctcgattt tagttttcat tgcctccact tcatctatct
     2041 gctttttatc tgtcacttta atttgactaa tgattaattc tgcttttttg gctagttcta
     2101 gggcggtatc aatatttata cttcgcacgc tttcgatttc tgtttcaatt ttattaattt
     2161 cttcttttgc tgtctcgatt ttgtttttgt tttcattttg aattcttttt tgataaccaa
     2221 aaaaagtccc cgcgattaat agacccaaaa caactaaact caaaattaaa ttaattgatt
     2281 tatggctttt ttgaagagac cgattttttt ctttaataaa aatatctttt ttattgttag
     2341 tcatttctaa cttgatagcc gccccttcaa aattttctct ttctcccaac ttatattctt
     2401 catcagcttt tttatctagt atccaaattt catcactctc ctttatctga ccagtgcttc
     2461 cgtcttttaa tctagctacc agattatttc tcttaatcag gacactacct tcccctttaa
     2521 tttttatcct tatttttccc cggttaattt cgacctcaat tatttctaat ccattttcga
     2581 gtcccaatct atttttttga tttatttcat tccatccatt ttctcgtgct ttctcgccga
     2641 ttagcctcgt taatcgatat ttcattaaaa aatgatacta cagaattgtc aaagccaaaa
     2701 acaccaacat aattgctcct agaaggtgta aataagaaac cgtaattaaa atgccattaa
     2761 atttatcttt tacattttta gacatggtgt tgacctgttt tactattacc aaggcaaaaa
     2821 ttaggtaaac gactgatccc gctattccta aaattttttc caataagtga aataaaccac
     2881 caagatcgtc aagcattaat tatttcctcc tttatctatt tctttgtctt cccgaagaag
     2941 ctgcaggatt ttaattattt tttccggatg ggggactttt tcaaaggtaa tataagacac
     3001 ttcggctgca gtttgaatgt gaattgtgcc gtaattaaac atagtttgcc cgagaccact
     3061 tactcttgag gtgacgtctt gaatcatgga caatttagcc tccgagaact ttttatcaag
     3121 tagattatta aaatcaatat caatcactct tttgtcggta atgataaaaa catcaaaata
     3181 ccaggagaga aaattttcaa aaataatcgc aaagcttagt aaataccaaa caaggtttcc
     3241 caggctagta tatttagtag gaatatcatt aattatgggg ataaatttta gtaaagccgg
     3301 ggcaattagc attaagatag aaattaacat ccaggaaagg ttggtaaacc aatggggcct
     3361 agcgactagc agaatctctt cctcatcatc tttttcttca aaactaaaga catcaggatt
     3421 gacaagaatt gaggataggg aatgcttggt atgaccgaca atttcatata ttttttcttt
     3481 taaattaact gttttaacag tatctgttgg tgtctctagt ttctcgtttc tcgtttcttc
     3541 cattttgttc tcaatcacgt catcgggtgc caccaaatct aaatcgtttg atcttgtcat
     3601 ttctttattt taacatttgg ttggcttcta ttaaatcctt gatatttttt gccaaaaatt
     3661 tagagttagc tctcttgggt aagtgcaaaa ataaatgttt cgtttttaat tttcttttat
     3721 taagtatcaa ttgagtttca aaagcaatcc agttgcaatt ataagttccc atattttccg
     3781 ttatttcaaa tatacttggt tcatacattt ctatttccgg taaatttagt tcaattttta
     3841 ttggtgctag tggataaatc gactctttgc cataaacatt tctagccact gtttcaattc
     3901 taattttttc tccatcaccg taaaaatcac ccaagcctat tatcaaatca taaacatcat
     3961 ttctaatata tttattggcg aaacttcgcg gatggccaaa aattatctgg tagtttattc
     4021 ccagactttg aggtaaatat tgctgcaact cactcaaagt tcttctggaa atattcgtgc
     4081 cccattgatt tgaaaaggcg taaattaaaa tcatcttttt tccacattat acaaaggccc
     4141 tacgatagaa atgttagctg gtgccctaat gttaaaaaga cttttaaaag ttgagccact
     4201 aactgaaact tgaccagcat caccagaaaa agaaagagtg tttgtttttc cttcctgttt
     4261 attccaatct gtcactgagg ctgagctgat tgaattaaat ggtgtttcac ctctattgcg
     4321 aagttctaat ttcactcttt ctgcatccca agtatcactg catcctgatg aattatcttt
     4381 ataacaaagg tgattttgta ccgaagaatc tttttgataa agcaaaacga cattcactat
     4441 atcagccacc tcagttggtt taagccaagc tgttttatta tattcagttc gatatccttg
     4501 ggccgaataa aaacaagggg attcccggtc gaaggctttg gcaaacaaat catcaaaaga
     4561 atcaatctca ccattggtat cccggactct tttagtccac cccgtagatc tccagccgat
     4621 atcggaagaa gtaaatgtat aaccgccggc agtagaggcg taccaagcag tgataatctg
     4681 accatcatga attaaaactt caccctcagt ttctctgacg gccctttccc agtttccacc
     4741 cttattgcca cccttataaa cttgacaact ttgagtggta caaatttcat ttgaaccatt
     4801 gttggtataa gccagggcat aagaccgagc cgcaaccact tgagatttaa gagcttctat
     4861 cggccatgat tctggaactt cataaatacc aagtaaatat gtttccagtg gcatagaacc
     4921 gaaaccttga acttgaatac taatatttgg ttttctctcg atagaaacat tgttatagta
     4981 agcgtttaaa atagttttat aatcttgacc agccttagct cgaccataag ctccatactg
     5041 atttaaacca acatgatggg ggataccaaa ggtaaagaaa gcaaagccac tcccaaaacc
     5101 aggatctctg gtccggtcat caacacatga aataccacca gagccgacac ttttaggtag
     5161 attcaatccg gctattcttt gggcttctag gtttattaaa tcctgactca atttagcctt
     5221 ataagtttcc gccttgctaa tttcaccact taaaaatcca aatctatctt ccattgattt
     5281 ttttaaagcc gctaatttaa ctttttggac ttctaactca tttttgtttt tattcaaatt
     5341 ttcaatttca gtcgaatagc tactgatgac atttttgtcc tgagaaatta ttgattgaaa
     5401 ccaggtatat tgctgcaaaa tattttcagt cccctgtgaa gaaaaaaaca tgactagagg
     5461 tgagaatctt ttcgagtcta tgtaatatct tctgactctt tgagacatta aaattttttg
     5521 aacctcaaga tcagcttcct tatcaattag tttttttcct agctcgctta cttgaaactc
     5581 gattccgcta atttgttttt tcgcactagc aatttttgcc tggagtccag tcgattcttt
     5641 ttttagaggc gcaattgctc cttcgaggtc cgctatctgt ttcttaatat tttcaatttg
     5701 attatttaaa gcatcatctg ccaatactct cccttttatc ccggccaaaa aacacattcc
     5761 taaaaaaaca aatattgcta taatttttag cctttctttc actctttcat tctatcatct
     5821 gatatacttt a
//
LOCUS       DHGB01000018            4206 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_130677, whole genome shotgun sequence.
ACCESSION   DHGB01000018 DHGB01000000
VERSION     DHGB01000018.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_130677"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1391 a          765 c          641 g         1405 t
ORIGIN      
        1 cgcccagcaa tcagttaaat ccaccctata tgctctcaat cctgcttttg ttaattttaa
       61 tttacttatt aaacagtaaa aatcctaaaa aaatatttat cttttttatt cttatctttt
      121 tacccattat aaaagtctat tccgccatcc cggcttttat tatttttgga ctctatgctc
      181 taaataataa aaaatatata aagaccttat tattatcaat cttttttgcc ctgattctat
      241 ttcttcaata caactcttct tcctcctctc ttataaaatt tgaacccttt tggtttacca
      301 attccatgat tgaatccatt gatcgtttct attggccaca agttgcatca tttttacatt
      361 cttcaaacat aatcaaatat atttttgccc aaatagtcgc cgtagcaata tttttaattg
      421 gtaattttgc ttttcgtcta ctggctgtaa aagaaatata caaagaaaaa agtatcttaa
      481 tttcgattat tatctgtact ttaatacctt tattttttgt tcaaaagggc accagttgga
      541 atacgatcca atttttatat tacgccctgt ttttagccaa tatcccttta tcggtttact
      601 tgtcaaaaca ttctaaatta acatatttag ttgttatttt aaacatattt cccttaattg
      661 gttcactccc aaattttacn nggcaaaata cctccaactg ccatttctaa atatgaatta
      721 ttagctttaa attttttaaa atcagagcca aaaggaattg tcttaaccca tccttacgat
      781 gcctatttac gaaaatcatt tccgaaaact ccaattccaa tttacgctta tgaaacaaat
      841 tcttatgttt cagcctatag taagcagcaa acatttttgg aggatgagat gaatttagaa
      901 aattccggct acaattggca gcctcgtcgc caacaagcgg aaaacttttt ttctcaaaaa
      961 aatgaatttg tagatagagg ctttttagtc aataatcaaa ttgattatat ttatctccct
     1021 aaaatttttc taaatcaaaa aaccgaactt tcacctaaat tatcaataaa aaatattttt
     1081 gaaaatcctg aaattgtgat ttataaggta aatagatgat aaactaaacg tatgcctaaa
     1141 agaccaaaca tttctgcagt gattaacgta cgcaatgagg ctgaaaattt aaacaaatgc
     1201 cttcgctcaa tccgagattt cgccgatgaa ataattgttg ttgacatgca ttcaaccgat
     1261 aacagtgtcg ctatcgccca aaagtataat gccaaaatat attcgtatcg tcccatgaaa
     1321 tatgttgaac cggccagaaa ttatgctctt tctagagcta ccggacgctg gattattcta
     1381 ctagacccgg atgaatattt aaacaagacc ctaaagcgag aacttaaaaa gatcaccgaa
     1441 cgaactgatg ttgattatgt ccacatcccc cgaaaaaata taatattggg aaaatggttt
     1501 cgtcacagca actggtggcc tgactatgtt cttcgctttt ttaaacgcgg ccatgtcgac
     1561 tggaaaaagg aaatccatag tcagccagtg gccaagggta ataatttaaa tcttttagat
     1621 tctgaaaaac tagccttaag acacaacaat tacacttcac tctctcaatt tgttcaccgt
     1681 gctcttcgtt acagtagtgt tcaggctgat gaactccata aaaataaata tcaactaaaa
     1741 acgagtgact ttattttaaa accaattcag gaatttaata gtcgattttt tgcggcccag
     1801 ggatataagg atggcattca cggtttagcc ttttccgtcc ttcaggcttt tgctattgcc
     1861 ctcatttata ttcgtctttg ggaaatcgaa ggagctgagg aaaaaaatat ctccaaagaa
     1921 tcttttgtct ctgcttccca ggaagccact tatgaatact catattggtt taccaagtat
     1981 tttcgccaag aatacaaatc tagtttttta aaagtttttc ttataaaaat aaggttattt
     2041 tttgatcgtc taaccaaaaa tttttaatgg tcacaagcgt tgccattatt attttaaact
     2101 ggaatcaggc ggatcttact attgacaccc tttcttcttt tttaaaaatt aaagcaaata
     2161 aattttcgta tcatatctat ttagtagata acgcctccac tgacgattca tttctaaaat
     2221 ttaaaaaatt ttataaaaat aatcaaaaaa ttagtttatt aaaaacgaaa tcaaatctag
     2281 gctacgccgg gggaaataat tatggtatta aaaaagcctt gctgaaaaaa tatgattatt
     2341 atttaattgc caataatgat attttagttg ccccagattt tttagaaaaa cttataaatg
     2401 caggtcaaaa aaatcctaaa tctatcctgt ttcccaaaat ctattttgcg cctggtttcg
     2461 aatatcacca agatagatat caaaaaaatg aacttggaaa agtcatctgg gctctcggag
     2521 gaaaaattga ttggaacaat gtctatgcat ctaatattgc cattgatgag gttgataagg
     2581 gccaatttga taatttagat ttaaaaattg attttatcag tggctgttgc ctgcttgtct
     2641 ctcatggtgt ttttaagaaa gttggcatct ttgatgaaaa atattttctt tatcttgaag
     2701 atgttgattt ttcgcagaga gctaaaaaag cgggttataa attacaacta gtttctgatt
     2761 cagttatttg gcacctaaat tcagcttcga gtagtcctga tagtcaccta caaaattatt
     2821 ttattacccg aaaccgcctg ctattcggtt ataaatatgc tcctattaaa acaaaatttg
     2881 ccctattccg agaatcaatt aaatttctat tttcaaatga ttcgtggcgg cgtactgccg
     2941 ttaaagatta ttatctaggc cattttggca aggggagctg gcaatgaaat tgagcattat
     3001 tattctcgcc ggcaacgaag ccgagatgat aaaagaatgc ttaatatcag cttctttcgc
     3061 cgatgaaata attttagttt tagctaactc ctccgacaat accaaaaaaa ttgccaagtc
     3121 tcttaaaata cctctaaaag tagtcgaaac taccgatgag tacaacaaaa attttagcaa
     3181 atggcgtaat ttaggctata aagccgccat cggagactgg ctactatatc tagacgccga
     3241 cgaaagaatc acgccagaat taaaatctga aatattatta actattaact ctactaatct
     3301 atttaactac tatgctattc ctcgagccaa tcattatctg gggaaaagag ttcgctacgg
     3361 cggttcttat ccggattatg ttaaaagact ttatgtaaaa aaacattttt caggatatcg
     3421 gggttatctt catgaagagc cgatggtaaa aggtgaaatn ntttacttca ctacactcat
     3481 cgaaatttaa attccatgct cgaaaaaaca ttggtttgga ctgatatgga ggcacaggcc
     3541 ttatttgatg ctcaacaccc ccctgtattc tggtggcgtt ttcccagaat gatgctgacc
     3601 aagacctggc aacgcctgat taaagagcaa atgtggcgtg atggcaccgt tggctggatc
     3661 tcagtcattt ttgagtcatt cgacaccttt attatttatg ctaaactttg ggagaaacaa
     3721 gtttatgact ctaaaagccg ccatctatga tccgtattta gatactctgg gaggagggga
     3781 gagatattgc cttagtgtgg cggaaatatt gcttaaaaat aaataccatg tcgatatttt
     3841 ctggtcgggg aatcaagata ttttgtcgat cgctgaaaaa agatttaatc taaatttgga
     3901 aaatctaaac attattccgg atatttttgg tttggtgcca gccaaaatcg acttaatcga
     3961 agatactaat ttgaaaacaa tccatagtcg ccccctagca actcaaaatc tttttcaaaa
     4021 gctaaaaaat atttatcaaa aatataagat tctgcctcaa tacgatttag tattttacct
     4081 aagcgatggt agtattccgc ttttattttc aaaaaataac cttcttcata ttcaggtccc
     4141 ttttattctc aaagaaaatt acctagataa aattttaaat ttgtttaaag ctaaatttat
     4201 taataa
//
LOCUS       DHGB01000019            5284 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_54980, whole genome shotgun sequence.
ACCESSION   DHGB01000019 DHGB01000000
VERSION     DHGB01000019.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_54980"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1362 a         1396 c          688 g         1835 t
ORIGIN      
        1 ctcgaaatag agcctccttg ttaaataggt caatgctatt actctccagt ctggctgctg
       61 ccgcctgagt cgccagtgcc cttttccaaa tcatttgata gagtttttct tctttactat
      121 caagcccctc gggactgatt actccaattt gtgtcggccg aatcgcctcg tgagcctcct
      181 gagcattttt cgaattattt ttataaaccc taacctttgg tgataaatac tcactcccat
      241 actttttttc aatataatcc cgaaattctt taattgcctt atctgataaa taaagtgaat
      301 cactgcggtg ataggttatt agccctcttt catagagacg ctgggccaaa gtcatcgttt
      361 gttttcccga ccagccaaaa cgccgagccc ccgcctgttg taattttgaa gtcgtaaacg
      421 ctggcactgg tgccttactc agttttttcc cgaatacctg actaaccgta aattctttat
      481 ccagggattc aataaagctt ttggcttctt tttcgttcgt aaataaagat ttggtgtaag
      541 taaaatcccc atcaaacaaa tttagctttt tcttttcaat aaaacttttg tttcctattt
      601 tgatgagttc tgcttcaaaa tttacctctt tattaaatag agccccgatt ttaaaatatt
      661 tttctttttt aaaggcttca atttctcttt ctctctcaca aatgagtcga actgccaccg
      721 actggacccg tcccgctgac aaaccccgcc tcactttttt ccataaaacc ggcgataatg
      781 aataacccac cactcgatca agcacccttc ttccctgctg ggcattgacg agattcatat
      841 caatctccct agctttattc aaagcctcct cgaccgcttc tttggtaatt tcgtgaaaag
      901 atacccggga aattttagcc gcgtcaattt ttcctttttg accttcagtc aacagccatt
      961 taacatgcca ggcaattgcc tccccttccc gatcagggtc agaggccaga aaaatatttc
     1021 ccgccccttt tgataatttt ccgagctgcg agactatttc ttttttcttg gaatcaatca
     1081 catattcaac cgtaaaatcc tttaaatcaa ctcccatttt attggtcggt aaatccctaa
     1141 aatgccctac cgtcgccatt acctcatagt cactacctaa atatttcgaa attgtcctcg
     1201 cttttgtcgg cgactccact attaatagtt ttttcataaa atacagtcta caccctttat
     1261 aattgcttat tgtaacatta ctcctctgct tgcccgtccg tagctttagc gaaggagggt
     1321 caagtcgtcc aaactaatta ttggcattgc cccttgtttt attaaccagt tggtcgcttc
     1381 actattttca tcagttattc tccccggtat gcaataaaca tttttatttt tttccacaaa
     1441 tttagacgcg agaagtattg ttccactctt ttctcctcct tctattatta ccaaaatatc
     1501 ggcataatct gctacctgct cattcaattt ttcgaaattc tccccctcaa aaatttttct
     1561 cgcgccaagc cttattacct cactattaca tccacttact ttaattgtca ctacttcctc
     1621 atctcttaat ttttccatta atattccaat tacttctttt ccataatcac tcatgtacct
     1681 actcccaaca atcgcgattt ttttcattct gttagtctcg gaactaccgt ctctggctct
     1741 tcaaaccact cttttaaaat atcaccgact attggtgccg ccacatcgct cccttctccc
     1801 cctctttcaa ccagcactgt aattgaaatt tccggattat cggccggggc aaaggcggtt
     1861 aaccaagcat gagtatcatt ggtgccatct ccaagctccg ccgtgccagt tttacaggca
     1921 atttctgttt taaaattaaa cagtggccag gccgtcccct cgctatcaca ggcctctctc
     1981 attccctcaa tcaccgccat ccaattttct tcttttgcct gtatttcggt acattttact
     2041 ggcttatttt taagtaaaga catttgacat ttttgccctt tactagcaat aatattagtc
     2101 atttgattaa tttgtaacgg ggttaccgtt aaatttccct gaccaattga caaatggtaa
     2161 gtatctccta gataccatct ctctcccaaa tttttccttt tccacccatc gtcaggtatc
     2221 aaaccagcca cttcccccgg tagctctatt cctgttactt gtccaaaacc aaatctctcg
     2281 gcccaatttt ttattctatc cactcccatt gcctccccca gtttataaaa gaaaatatca
     2341 tttgatctct ttagagcttt gaccacattc accatcccgt ctgtggttcc tctttttgtc
     2401 caaagccagt tattataagc ataatcgcca attttaatta ctcccgtatc ttgaatcagt
     2461 gtttctctac ttatcacccc ctcttcgaga gcccctagag cctcaactgg tttaaacaca
     2521 ctcccaggat gatatttagc tgcaatcgtc cgattcagca tcggtaaatt tactgtatct
     2581 tcaagataag ttctgacagt ctccttgtca taactataat taaaatcatt ggcatcatag
     2641 tttggtgaag aaactaaagt aataattttt cccgttttcg gttcactcat aattaccact
     2701 gcctttttat cctcaagcat tttataaatt ttgtcctgcc aaaaggcatc gatacttaga
     2761 gcaacttctt ttcctggcac tggatcaagt tgtccgaggt cccgattgct gttacccacc
     2821 gcatcaacct caaccaggcg atagccatca cttcccaaaa gctcacagtc catttctttt
     2881 tccattcccg ctcggcctac cacactcaaa ttaccaagtt gttggccgca ttttccattt
     2941 tttaactcat cttcgttgac ctttccaaga taccccgtta catttgacat tgaagcccca
     3001 taaacataaa aacgctttat ttctccgtta aaatcaatat tttccgctat ttttcttcct
     3061 ttccggtcaa caatttctcc cctttttgcc ggcaaaatta tttttgtaac ccgattattt
     3121 aatgccaaat tacgataata agggccctta ataatgttca gtttaatcac ggttacaaag
     3181 attattccaa aacttaaaaa cagggctgcc ttaaaccacc aaaatttcat tttaagataa
     3241 atttgatact aattttattc tttcaagcca ccctttattt tccagtaact ccccctcccc
     3301 tttaaccact ccaaaagcag gactgggcaa cactgaggcg tttattttta cctcttcctc
     3361 aattaaagtg tcaattcctg gaaattgaga tccacccccc gacattaata cccctctttt
     3421 taaaacatcg tccgtcattt cggttggcat ttcatcaaga acaattttta ctaaattgac
     3481 tattcttttt acatttaaag ctgtcgcctc attaatctcc tcggctcgaa gccgaatact
     3541 ctttggtaaa tttgtttcaa ggtctctccc tctaatcact ccccccatct caatttttac
     3601 cctttcagcc gtattttttc caataaaaat cccgtatttc atccgaacat atccaacaat
     3661 cgactcatca aaatcatcgc ctcccatttc aaggcctcga ctaatcacca ccccacccat
     3721 tgacaccaga gaggcttcag tttttccccc accaatatcc aaaaagagca ctcccccact
     3781 ttcatccatt ttaaaaccac tcccgaggat tccgagaata ctactttcaa ccataatcac
     3841 ttttgggact cccacctgtg ccagtattgc tcgaaaagcc ctcctttgga cattactgat
     3901 agttcccggt actcccacaa tcactctcgg ttttaatatt tttggatagg agctcggaat
     3961 ttcattaatc agtttcataa aataagcgag cagcgcctgg gcactaacca gatcagcgat
     4021 tgccccgcgc tttatcgggg ccaccacttc aatttgtttc ggctcccgat tgaccatctc
     4081 cttagctttt tgaccaaaga ccagcatttt ttgaattcca tttttctttt ttttgagtcg
     4141 cgctatcatc gaagcctcat caaccactac cccccgatct ttaacataaa tttttacatt
     4201 actactcccc agatccaaac ctatctccca ataaaaatta gaaaaaaaat tagaaaaaag
     4261 cttcatttca tcagtataac aattactgct aaaataatca ggtgacttct attgttcaat
     4321 ttttaatttc gattctcttt ttttttactc ctctcatttt tacttccatc tcctctgagc
     4381 tgtttgaagt cccaaaaata tattttgttt atttatcaac tctcttaatt actttatttc
     4441 atttatttaa ttatttaaaa ggcaaaactg tcttattttc aaaaaataac ctaactattc
     4501 ctctacttat ctttttttct actcaactaa tttctaccat tttttccgtc gatattcaca
     4561 cctcaatctt tggttattat tcacgcctta atggtggcct actttctctc agtgtctatt
     4621 tattactatt tcttattctg cctctttatt tcacccccaa atttcgtgac aaaatcgtta
     4681 atcttatttt aatatctggt tttctggtct caacttttgg tattttggag cactttggca
     4741 ttgataagca tctttgggtt caggatgttc aatcccgggt tttttccact ctcggccagc
     4801 caaactggct cgccgcctat ctctgcattc ttcttccctt tgcccttcat aaatttcttt
     4861 ttgccaaaac ctttctccaa aaatttagtt tcttgtttct cgtttctaat ttctacattt
     4921 gtcttctatt taccaaatcc aaatcgggac tcatcgctgc catcatttct ctggcaattt
     4981 atttaattat caannnaatc ctatcaaaga cttttttatt aaaccaaaaa tatccaagcc
     5041 agtctctcaa aatacctcat taaacatcac cccgagtgaa gacattcgta aaattgtttg
     5101 gcaaggtagc ctcgacttat ggagggcttt ccccctcttt ggcaccggcc ccgaaacttt
     5161 tggctttact tattactgga cccgtcctgc ctctcacaac ctcacttctg agtgggattt
     5221 tctctacaat aaagcccata acgaatattt aaattatcta gccaccactg gcaccttcgg
     5281 cttt
//
LOCUS       DHGB01000020            3268 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_117430, whole genome shotgun sequence.
ACCESSION   DHGB01000020 DHGB01000000
VERSION     DHGB01000020.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_117430"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1029 a          624 c          613 g         1002 t
ORIGIN      
        1 tcgtcaacca catgtttctt cgccttttct tcggttctac cccttgactt gatacagtca
       61 cgcctgttat tacttaatta tgaaaaataa aaaagtaaaa actgtaatat ctcatacgag
      121 ttcttctctt caagacaaaa aaggtgaagc tgtgcgttta gccatggagc aaattaccaa
      181 agcctatggt gaaggtgcca ttatgaaaat gggtgatcgc actgatatgg acattgaagt
      241 tattcctact ggctgtttac ctctcgatat tgccttaggt gtgggtggtt tacctcgtgg
      301 tcgagttacc gaaatttttg gtccggaagc ctcaggtaaa actactctct ctcttcatgt
      361 gattgccgag gctcaaaaaa tgggaggtac ggccgctttt attgacgctg agcatgccct
      421 tgagccagtc cgcgccgcta ctgtcggagt tgacctaaat aatctcttaa tctctcaacc
      481 cgataatggt gaacaggcct tagaaattgt tgaaactttg gttagatcaa atgcagttga
      541 tgttattgtg gttgattctg tcgccgctct agttcccaaa gccgaaatag agggcgaaat
      601 gggggattcg atgatgggag ttcaggccag gctcatgtct caggctcttc ggaagttaac
      661 tggcgccatt agtaaatcaa agactgtggt tatttttacc aatcagttac gtcaaaaaat
      721 cggtattatg tttggtaacc ccgaaactac tcctggcggt ctcgctctta aattttattc
      781 atcagtccgt attgatgttc gaaaaattgc caatatcaaa actcccggtg gagaatcaat
      841 tgggtctcag catcgggctc gcattgttaa aaacaaagtc gcccctccct tcaaagaagc
      901 cgaattcgat attatgaata ccgagggtat ttccgtcaat ggttctctcg tcgacatggc
      961 cattctttat ggttttatca ctaaatccgg cgcttttttc aaaatcggcg accaaaatat
     1021 tcagggccgt gaaggtgcta aaaaatatct caaagaaaat cccaaaatga ctaaagagct
     1081 gacaaagcaa atttgggaaa aagttaaaaa cgatatcaat aatgccactt caacttcagc
     1141 gccttctgcc gagtcgcagg actaaaaatc gagtttggct aatatttagc gattctctca
     1201 agctgccttt ttacgctgat gatgtcttaa aactgggtct aaaagcgaat ctagatatta
     1261 gtgataagct ttttgaaaat ataaaaaatc gctcccttta ttacctactt tataattatt
     1321 ccctaaatca agttgctctt tctccaaaaa tacaacaaac attattaccc aagcttagac
     1381 aaaaattata tttttatcaa aagaaatata aattaaccgg agattttcaa attttggttg
     1441 acgaaattat cgataaatta tcatctttaa atttactcga cgaagcttct ttttctgaat
     1501 atcttttacg aaaaaataaa aaacgttccc agcagtattt atcgagactt ttttcttttt
     1561 atcatttaac ctcacctcaa aatactcccg agggtgatat tcaaaatatt aaagatattt
     1621 tgctcaagaa aaaataccta tctttaaatc tttcggataa cactgttaaa aacaaattaa
     1681 tcacttcttt attcagaaaa ggttttgcct ataatgacat taaaactgta attgacgaat
     1741 tgagtaaaaa caagtaaact atcattgttt aaattaattc tttatttata tatttcactt
     1801 tttactatca actctaaaat gatctcaaat ttattctttt ctggtttttc gctggattta
     1861 gagtaattct tgccgataag taaaaggtgt ggaggtttac tatgtttaat tcatttcttt
     1921 caaaatttag gagtctttta aattcttcta attcaaaatt acccgtttct gacacaattt
     1981 ctactccctc tgtcggggat gtcgacaaca accgagttgt tttttcccca aaaaaatcag
     2041 caaaagttga aaagattaaa aaaaatgatg tcgctctttc tattccagtt aataattcag
     2101 tcgccgaagc tcaggcaaaa gtaattatcg aaaatgcccg tcgtcttgaa gccgaagcca
     2161 aaactaaaga agccgaaatt tttcagagat tatcttcttt aaacgacaag gaaaaatatt
     2221 taattcaaaa agagcaatct ttggatactc agcaggccga tcttaagaaa aaaattgatc
     2281 agatcgacga aacttatcgg aaacaacttg aaaaattaga aaatatttct ggtctcgatg
     2341 tcgacaaagc caagcagctt ttgatgagta gtgtcgaaaa gaaaatgact tcttggctcg
     2401 ccaaaaaaat tgatgagacg aaggatgatc ttagttctcg tcaggaggaa ctagccaagg
     2461 aaattctcag cgaggcctta actcatggag ttaccgatta tgtggctgaa tacactgttt
     2521 ccaccattac tcttcctgat gaaaaaatta aaggaaaaat tattggtcgt gaaggtcgca
     2581 atattcgcgc ttttgaaaaa gctaccggag ttgaaattga gctagatgag accaacgata
     2641 tccgtatttc ctcttttgat tccactcgtc gggaaattgc ccgcctaact ctcgaaaaat
     2701 taatcaaaga tggtcggatt cagcctgtta agattgaaca attagtcgcc cagactcaaa
     2761 ctgaaatgga taaaatttta ttgaacgagg gtaaaaaaat ttgccaggaa ataggtgttt
     2821 attcacttcc agttgattta atcaaagaaa tcggtaaatt taaattccga ttttcctatg
     2881 gccaaaattt agccaaacac actgttgagg cggttaaaat tgcggtagct ctagcctatg
     2941 aactaaaagc ggatgttaat acagtcagac ttggtgctct ttttcacgac attggcaaag
     3001 tggtcaccga tcaggagggg actcatgttg aactgggaat tgatttgctt cgtcgttaca
     3061 aaatatcaga agctgtcatt aatgcggtcg ccgagcatca tgaggacaag cctttctctt
     3121 cagtcgaatc ggttctggtc tatctgggtg atgctgcttc cggagctcgt cctggagctc
     3181 gttacgaagt ccatgaagaa tatttaaaga gaatgcaaaa cattgaggaa gttgctaaag
     3241 gttttcctgg agttatcagt gtcgccgc
//
LOCUS       DHGB01000021            3187 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_177079, whole genome shotgun sequence.
ACCESSION   DHGB01000021 DHGB01000000
VERSION     DHGB01000021.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_177079"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT          886 a          590 c          377 g         1334 t
ORIGIN      
        1 gctctcgatc gaaaatggtg gatggttacc cttatgccag tcatactagg cattatgcgg
       61 tatggccaaa tcatctttga aatgcaggag ggtgaaagac ctgaaagagt tattgccacc
      121 gatattccct tactcggctc agttcttctt tggggaatga tgataattac tattatctat
      181 gttctctaat aggaaatttt tttctggttt tttaattatt tttattgtct tgtcgtctat
      241 ttttacccgt ttctacaaac taaactgggg taatgattat tattttcacc ctgatgaagg
      301 taatatggcc tcggcccttt cccgactaca aactaacgat cttgatccta aattctacgc
      361 ttacggtcag tttcctttgt atttgggttt ttttacctta aagatattta acatttctaa
      421 ttctttttcc aattcaatct ttatccttcg tttctatgct gctctatttt caatattgtc
      481 gattggttta ttatattttc tttctcaaaa gattttttct tcaaaatttt ctctcttggc
      541 aacccttttt acaatttttt ctcctggtct gattcaaata gctcattttg gcactaccga
      601 aagtttacta attttcatat ttttattaga aatttattta tcaattacta tttatcaaaa
      661 aaaagaaaat tactggcaat atattcttgc cggtatcttt ggtggtatcg ctctagccac
      721 caaaatttcc tcattaatat ttttcggtcc cattttttta gttactttga tgaagttaat
      781 caaaggggaa aagatattgc caattatttc aaaaattata ttactaatag tttttaccct
      841 aaatttttac ataatttcct ctccctataa tctcgctaat agtcaagatt ttttgtcttc
      901 catgagatat gagaccggtg tagccacagg ggagtctcga gttttttata ccacccaatt
      961 taaaaatact ttttcctatt tatttcaatt ccaaaaaata tttccctatt cttcaggtat
     1021 ttttcagttt atctttgctt ttattggtct ggttctttta ttaaaaaatt ggaaattaaa
     1081 agaagataaa catctttact ggtttattat tttaattcct tctctggttt attttcttta
     1141 ttttggtcag ctttatgtca agtggactcg ttttgtttca cccctatttt ttctttttcc
     1201 ccttctctca acatttttta tctctcttct aaaatctcgt ttctgtcgtc tagtttctat
     1261 aattgttgct tgtcttccag gcttatattt tatgaaaata tattttaatc ctgacatcag
     1321 aattgttgcc tctagctgga tagaaaacaa tattcccgtt aactcaaaga tcctttctga
     1381 gggtggcaat gtaattaata ttccaattac cgacaaaaat taccagatta ctaattatga
     1441 tttttataat aactatccaa acaacttatc taaccatcta ttaactacca actatataat
     1501 tgttccctct cgccgggttt ttaaaaatta tgatctaaaa tattatcaaa atcttttcaa
     1561 tggctctctg ggttttaggc aaattaataa aatctcaccc gactacgatt tatttttaaa
     1621 tgccgaggac gctgaagaaa cctggtctgt ctttgatcat cccaccattc ggatctataa
     1681 aaaagttaat cagttgaccc tagaagaata tgaagctttt ctctaaaaat atattaatta
     1741 tattatcgat ttttatttta gccttcttac ttcgtatcta tggccttaac tgggacgaca
     1801 accatcatct tcatccagat gaaaggtttt taacgatggt cgttagtgat ataaaaatac
     1861 cactctcttt atctcaatat ttcaatacaa ctacttctcc tctcaatccc tacaactact
     1921 ctcagtatca attttttgtt tacggtacct ttcctatttt tttaacgaaa atagtagccg
     1981 tattatttca tctagatgat tacaatcaca taactcttgt tggccgtgtt ctctcggcct
     2041 tatttgattc cggaaacatt attcttttat atttcatctc taaaaagttt ttaaagaaat
     2101 attacgtcta cctcccctca tttttttata catttttagt tcttcctctc cagctctctc
     2161 atttttttgc tgtcgatacc tttttaactt tttttattct ccttacgttt actctatttt
     2221 cctataatct ctttgctttt gctttttttg cttttggttt agctcttgcc tgtaaaattt
     2281 ctgctctcta ttttattccg ataatttttc tatttttgct taaaaaatta tttcgcataa
     2341 aaaataaatt ttccctttta ccattaactg tttactactc attatttact ttgctagctt
     2401 tcagaatttt tcaaccttat gcttttacta atatttttaa aatgaatcct ctctttatta
     2461 ataatcttaa aactcttact aatttttcga gtcctgatat ttattatcct cccgcagttc
     2521 agtggattaa tcgacttgcc cttttaaatt ctttagccaa cttaagtatc tggggctttg
     2581 gaattcctat ttcaatttta tttttttcac tgattatcat ttttttcaaa aaaccaaaat
     2641 taaaatttaa tcttactttt attatttgtt tttggatttt atttctctat atatatcaag
     2701 gtagtcaatt ttcacactca atgcgttatt tccttccaat ttatcccttt atctgtctaa
     2761 tctttgtttc tcttttatca catctaaaat taaaaaaata ttatattatt cttgttttta
     2821 tttttaattt aatttatgct tttgcctttc tttctattta ttcacgaact aattctcgag
     2881 tacaggcatc aaattggata aatcagaata tttctagaga tagtattctg agcagcgagt
     2941 actgggatga tgctctgcct ttgggctatt cagaatataa aaatatttcc cttcctcttt
     3001 tcgatccaga tagtgaagaa aaatgggaaa aaattaatca gtctctcgat cagatagatt
     3061 atttaattat gtccagcaat cgtctttggg cctctattcc tcgagttcca gacaaatatc
     3121 caataacatc aatattttac gaaaatttgt ttaccgaaaa attaaatttc aaaaaaatta
     3181 aagaatt
//
LOCUS       DHGB01000022           20533 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_3296, whole genome shotgun sequence.
ACCESSION   DHGB01000022 DHGB01000000
VERSION     DHGB01000022.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 20533)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20533)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20533
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_3296"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         6605 a         2932 c         4753 g         6241 t
ORIGIN      
        1 tggtaaataa cggtgaaaac attctgaggg aattatagca gatttttgaa aatcaatgag
       61 gggaaaatgc ggaaaatcaa atctcccttg tattccccct ttgaggaaag ggggaaagaa
      121 tgaataataa atgtcttaat tttttccctc ctttattcaa aggaggggtt ggggtagatt
      181 ttatctcaaa aagaaataat ccagggctga tttggggtcg acattagcat cgataaattt
      241 gagggcagta ataatttttt taataatttg agcagtcttt tttgtcggat ttaaaaccaa
      301 ttgctgctga taaacttcta gctgggttaa aagcagattt tttatttcgt ttttgtcgtt
      361 gacaatagag gcggcaaaat cgagatcttt tttaggattg ccactgagag tccagggctc
      421 agtctgagag ttaattttgg gagaggtaag tttaatcttt tgacagcggg ataaaatagt
      481 ggggaggagt agttgaggtt tggtggtcgt taaaatcaga tagttatttt ttcccggttc
      541 ttcaagagtt tttaaaagag cgttttgaga ttcgggattt aaattatcag cctcggagat
      601 gagaacgatt ttagaaaaat gattaaatgg tttttgggat aagaaatttt tgagaaggcg
      661 gacattggca atcgtgtatt cgtttaaaat aaaaagatcg ggattgttgt ctggaagatg
      721 attaagctgc gagagaaatt caagggcttt tttcaaggaa acttgggggg aagaggcaat
      781 gattagggtt gaaggaaaga gtgacatagt ttactatgat tatagagtat gaatggagac
      841 gcaaaaatat ataaaacatt ttcggcagtg ctactcgata aaggtcttat cgataaggcc
      901 aaggcggagg aaattaattt aaggcaagtt aaaacaggag aaagcgaaga ggatattatt
      961 aggagtctcc gaattgtaaa cgatgttgat tttgttaaat ataaggcaga atttttgaga
     1021 attccctttg ttgatctcga aagtattggt ttttcaccgg aggctctggc tctggtacct
     1081 caaagtgtgg ccgaaaaata tcatctagtc ccctaccgga tggacgccaa agaaaaaact
     1141 atttacgtgg ccatggctaa tcccctggat attgaaacgg tggagttttt ggaaaaaaag
     1201 acaaatctga aaatagaaac ggcgatggcg gtggaaaagc aaatagacgc atttatccgg
     1261 gaaaaatatg aaagggaaaa aggaattacg actgaggtga ccagggctct tgacgaacaa
     1321 aaggtggaag aaatgaagac agaatcggaa aatttaaaag cggtttcagc cgaagcgcca
     1381 attgccaaaa ttgtgaatac cattcttgac tacgctgttc gctccagagc ttctgacgtt
     1441 cacattgaac ctatggagga aaatgtcaga gttcgttatc ggattgacgg aattttacag
     1501 gaaaaatata tattacctcg aaatgtcttg gaagcggtta ttagccggat taagattctg
     1561 gctaatttaa aaatagacga aaaaagaatc ccccaggatg ggcgttttaa tatcaatgtt
     1621 gaagggagcg aggtggattt gcgtatctca actttacctg tatcttatgg tgaaaaagtg
     1681 gtgatgcggt tactccgaaa agcctcgaaa gtaccttcgc tgccagatct cggacttcga
     1741 ggtctggctc ttaaaaattt aattgaagca attgagagac ctcatggaat tatccttgtg
     1801 gtggggccga caggttcagg taaaactacg actctttatt cggttcttga taaggtggcg
     1861 acctccagag ttaatgtggt gaccattgag gatccggtgg aatatcaaat gaagggagtt
     1921 aaccaggtcc aggtaaatgt tcaggcgggt ttaacgtttg cttcggcttt acgttctttt
     1981 ttacggcaag acccaaacat tatgatggtg ggagaaattc gggatacgga aacggcggag
     2041 ctggcggtca atgcggcctt gactggtcac ttggtttttt cgacactgca caccaatgat
     2101 gcttcggggg cggctccaag aatgatggat atgggggtcg agccattttt gctaacctcg
     2161 gctctaactt gtgtggtggc tcagcgagtg cttagaaaaa tttgtccaca ttgtcatcaa
     2221 gatatggaaa taccactgga taaagaggcg gaaatgaaaa tgactcttgg accgatttat
     2281 gaaatgattg aggatagatg gaaaaaagag ggcaaaaaaa tgactattcc caagattgtt
     2341 ggctgtgata agtgtaataa tactggatat ttcggcagaa ttgctattta tgaagtgatg
     2401 ccgataacgg aaaagataag taaattaatt gtcgaaaaag cctcagcttc ggatattcaa
     2461 aaacaagcta tttcagaagg aatgctgacc atgaaacaag atggttacgt taaagtcttg
     2521 gaaggagtca cgagtattga tgaggtcctc cgggtagcac agtactaaaa aaaagaaaaa
     2581 aagtctaaag cctaaaatct aagataaaaa aaataaaaat aaaaaaggat agaattaaaa
     2641 tataaaatta aaaatttatt tgactttaga atttagattt tagagttttt ttaaacaata
     2701 tgcatagcaa aatagaagaa gttttaagat tagcagtagc caacaaagcg tcggatgttc
     2761 atttgtcggt tggcttctcg cccaaaataa ggattcatgg agagcttgcc gatgtctcga
     2821 acattggagt gatcgaagac gaaagtttga ttttgtcact gctctcagag gagcaaaaac
     2881 aaacttttta tagagaaaaa gaacttgata tttcagtgac gattgataag gctaggtttc
     2941 gaactaatgt ttataccaaa aaaggagtcg cggcggcggt tttacgggta attcaggatg
     3001 aaattcctga tttaacggct ttaaatttac cnnggacacg gaaaatcgac gacggtggcg
     3061 tcgatgataa atgaaataaa taaagaaagg gcggtcaata ttgtgacgat tgaagatcca
     3121 gtggaataca taataaagcc aataaagtcc ataatttctc aaagagaaca tggaactgat
     3181 actcttaatt ttgccagggc gttaaaatca actttaagac aggacccaaa cgtggttttt
     3241 gtgggagaaa tgagagattt ggaaacgatt caactggctt tgacagtggc ggaaaccggt
     3301 catttggttt tttcgacact tcacaccaat tccgcggctc aaacaattga cagaattgtc
     3361 gatgttttcc ctgagggggc caagccgcaa attagacttc agctggcctc ggtgctttcg
     3421 gcggtaattt cgcaaagact ggtgccagcg attaacggtg gccgagtgcc ggcggtagaa
     3481 attttaaccg cctcaagcgc gacggctaat acgattagag aggctaaaac ttttatgatt
     3541 gacaatatta tccaaactgg agtggacctg gggatgatga gcctcgaaat gtcactggca
     3601 tctctagtca aaaaaggttt gataaccgaa gaaacagcaa tgagctattc tttaaaacca
     3661 accgaaatgc aggcaaattt aagaagttta aaaataaata aataataaat ggcaaagttt
     3721 gtttatcggg caaaagattg gagcgggaag ttgcttaaag gacagctcga tatggttagt
     3781 aaaaaagaag tagttgattc gatcaaaagt agtggattaa tgcctttgtc agttgaattg
     3841 gttaagggca atatgctgac tgaagcaatg aaaagattta agggtaaggt taatttaaaa
     3901 caggtggcta cttttactcg acagctatcg accatgatga cggcgggttt gcccctgacc
     3961 gatgccctcg ccttactgaa aaatcaacag gatgagacga gtggtcttta tgaaattctt
     4021 gacaggagtc ttgaacaggt tcgggggggg atgccttttg gaaaatctct cgagccttat
     4081 caaaaagttt ttggtgaagc ttatgtagct tcgatttcag cgggagaaga agcgggggtg
     4141 cttgacgagg tgctgagtaa attggcgaaa acgatcgaag atcagtatga atttcaggga
     4201 aaagttaagg gagcaatgat ttatccggta attgtggtga ttgccatgat tggagtggta
     4261 attttaatga tgatttttgt gattccaaaa ctgatgggac tgtatgctga ttttgggaca
     4321 gctaaaatgc cggcggcgac acttttttta atgtcgatgt ctgattttat gattaaaact
     4381 tggtttatga ttcctatttt ggtgataggg ggggtggctg gttacaacgt aggtatgaaa
     4441 aatgaaaatt ttcgtttaaa aaaagataag ctgaccttaa aaattccgat tgccggagtg
     4501 ctctcagaaa aaacaatttt ggctaatacc tgccggactt tatcaatgct tttgactgcc
     4561 ggcattactc tggtggaggc gctgaaaatt accgcatcgg tatcggggag tgaagttttt
     4621 aaacaagcct atttggaaat ttcggaaaag gtccaaaagg gattttcggt agcggtatca
     4681 tttcaaaata cggctgtttt tccgattatt gttaatcaga tgatttctac aggggaggaa
     4741 acgggaaaac ttgatgatgt tttaatgcgg gtatctgatt atttttcaaa agaagcggaa
     4801 gaatcggtga aagcactaac ctcagccatc gaaccactga ttatgatagt actcggtctt
     4861 ggagtcggct ttttaatgat tgcggtagtg atgcccattt ataacttgac ttcctcattt
     4921 tagtagtaaa taatgatatt aataatttaa atatttattt tttaatctaa atgaaaaaag
     4981 catttacgtt agtagaactt ttaatcgtta tcgccctgat cgcgattttg tcagtggcag
     5041 ttttggctac tattaatcct attgagcagt cgaataaagc aaaagactca acggtccaaa
     5101 atgacgcagc ggaggtgatg aattcttatg aaagatatta tgctaatgct caggtttatc
     5161 cttggatgct ttatggacct atcggttcga agccaactgt tgaggacccc gcattattga
     5221 gatcagatga tattggtttt ggtttgtgtg atgctggcat tttaactgat ccagcaaata
     5281 cccaagctat tgaatgtgag gtgactagtg aaacgccagg tgaattaatt aaaagtgatg
     5341 agcttaaaac tgcttttgtg gggaaagatg agtttcggac tgtggtagat aactctcatc
     5401 cggaaaatgc tttgtggtta tataaacagg caggttcggg tggtggtttg tatgtttgtt
     5461 atgtaccaaa ggcaaaatct aatagaaatc ctgcagccaa tcaattattt tgcttaacgg
     5521 gtggagctac gcccattaga actgcaaatg ggacaggtaa ttgtactgca ccagcaactg
     5581 atagtactgc atggggtgat ccgacagctg cgattgaagc aggtgcggcg gtagatgcag
     5641 caatatttaa gtgtgtaccg taaaggacat tttgaagtga attggacccc gccgcagtgg
     5701 cggggttttt gtttagtttc tgggcgcaat aaattgcgcc cctacagtag gggtttgatt
     5761 aatcaaaccc tgaatttaat gtaaaatgga tttatggaat tctttttggc agtaatggtt
     5821 tttgtactcg gaatgtgcat cgggagtttt attaatatgg cggtttaccg tatcgcaaag
     5881 cgatacgaaa ttatcaatta tcaattatca attacgaatg ataagagaag ctactgcgat
     5941 ttttgcggga aacagctgag ctggtatgac aatattccgg ttttttcttg gctgttttat
     6001 cgaggtaaaa gccgatgttg tgggaaaaaa ttaccactcc tctaccctat cgtagaaata
     6061 agcacagggt gcttatttct actaaattat caattatcaa ttttcaatct acaatcgatt
     6121 gttgtttata tgattcttgg attgctggtg tttgaggcgg tctttgattt taaatatatg
     6181 gttatcccgg atggaagtgc ttacgcgtta attggattgg cggccatcag gtggataata
     6241 ttaggttcaa aaataggata tttatatacg gcccttttat cggctttatt tatttttatt
     6301 ttgcacaaaa ttaaaataaa aggcaaagaa accatgggtg atggcgatat atttctggct
     6361 ttctttatgg gcttattttt gggatttccc agtattgtaa tggcttttta tatcgctttt
     6421 attgtcggag cggtggtggg aattatttta attatgaaaa agaaagttaa gaagctctcc
     6481 ccgattccct ttgggccatt tttaatccta gggatagtgg tggcgttctt ttggggtgaa
     6541 gaaataataa aaataataaa attctaaagt ttaaaaccta aaaccaaaaa aagcctaaag
     6601 tctaaaacct aaattctaaa taaaaataac aaaatataga aattaattat aataaagatt
     6661 agaataatgt tagtgaaaaa agataagggg tttacgctgg ttgagatttt ggtggtgatg
     6721 gcgattttat tgattatgat tatgatttta attggaattt taaacccaat tgccttggta
     6781 aataaagctc gagatagtcg aagaaaaaaa gatttgagac ggataaaagt agcttttgaa
     6841 gaatattata atgataaggg atgttatcct aatcaatcta ttgttgatgg actaatgcaa
     6901 aaaagttatt gtggaggaag ttctattttt ggaagatggc taaagccttg gccttgtgat
     6961 ccaaatggaa attattacga aattcaggta ggttacgatc aaaattgccc aaaatggttt
     7021 agaattttga cgatactgga gaataaaaat gatatggcta tcaatatggc gggagtttta
     7081 gcaacagatc ctaattacgc tgtctcgagt gacaatatac ctgttaactt ttatccagga
     7141 gataataatc catcttgtga atcaaattgc tatgtggtgg tggacgaagg tttgacctgt
     7201 aatagtcgta acttctcaat gggttgtgag gctccaaatt gttatagaaa ccctgattgt
     7261 actcctgaat gtcaaacatc ttgttgtggg gctgggtgtc cataatattt ttaaataatg
     7321 atagaattaa ttaaataaga tgagaaaggc atttacttta gtcgagcttt tggtggtagt
     7381 ttcaataatg gcaattttga cgattgtaac ggtgtctcag tttagaacgg ctcgaaagag
     7441 agctaacgat gtggccagaa agggtgattt aaactcggta gccaaggccc tgcaattata
     7501 ttttgcagat tatggaataa tgcctgaagc agttgacggg caaataaaaa cggatgtaat
     7561 tagaaaatgg ggggaagagt ttaaagatag tactggttat atctatatgg taaaacttcc
     7621 gacagagaat accgatggct ggccacaata ttgttataaa actgacagta ccagaaaaaa
     7681 atatgctcta tttgccaagc ttgaggtgtc aagcgatagt gaatgtatcg atgctaataa
     7741 caatggagtt ggggatttta tttgcggtct atctatggcg gcatcagaag gtccatattg
     7801 ttatgtcaat gctagtccta atgctcgttt aaatggaaag ggagactttg aaccatgaaa
     7861 aaagcattta ccttagtcga attactggta gtgattagta taattggaac gctggtggca
     7921 attttacttc ccaactttat gggagccaga gagagagcta gagatgctca aaaaattcaa
     7981 gatgcctatg ctatcaagaa tgccctgagg atgtattaca atgataatca ggcttatcca
     8041 actccaaatg cgggcacagt cgttttgcca actagctata tggctccgag cggattaggt
     8101 tttacttatt cacaaactac aactgatgat tttattatta gagtaccact ggaaagtgga
     8161 cagggggacg aagatttaaa ttcacaagca aaatgtgggg ttggagccac tgataaaatt
     8221 tatgctgtct gtgctaatta aaaaagtcta aattctaaag tctaaaaatt aaaaataaat
     8281 taaaaattat aaaaatatga aaaaaagagc gtttaccctt tttgaactac tggttagtat
     8341 ttcgattatt ggaattttga tcgcagtggc gtcgatgtct tatggtagcg ctcaaaaaaa
     8401 agccagagat acgagaagaa ttgaggatct taaatcaatt caaaaggcaa tggaaatgta
     8461 ttatagtaac aataatagtt atcccacgaa taatttccaa actaatttgt ctccatattt
     8521 ggtttggcca actggtccca aaggagaaac atatcaacct attatatatt tatcttcaac
     8581 ttattgtatt ggtgtgagta tggataatcc aattggcaac tgtaattcat catgtaattc
     8641 aggaactagt cattattgtg ttaaaaatca acagtaaaaa tagattaata gattaatagt
     8701 taattagata aatagtaaaa aagtgaaaaa gaataaaaaa ggatttagtt ttattgaact
     8761 gatagtggtg attgcgatta ttgcggtgct gagtgtgacg gcgatattga gttatggccc
     8821 aagtcaacga aaatctcgtg actctcggcg tatggccgac ttggaaaaaa ttcgaatggc
     8881 cctggaaatg gccagacaaa taggaacaac ttatccctcg gccttaccta ctttagttag
     8941 tcttggtgta attcaaacta ttccgactga tcccaaaaca aaaactaatt atgtttatgg
     9001 tcctactcct ccctattata aatattccat tggcgcttcg atggaagatt tgggatcaac
     9061 caatatacca gctagcggcg gtttaaatta taaggtattc aacccttaat tgtgataaag
     9121 ttttattaat gaagagagct tttactttag ttgaattgat agtctcgatg gtgattgtga
     9181 tgctgatttc tggtggggct ctggtttatt taaacgaatt taattcaaga caaaaactgg
     9241 aaaaaggaaa agatgaagta gtggcggcga taaaaatgat tcagagtagt gccaggggga
     9301 ggcaattacc tttgaattca acaaagtctg aattaaactt tattcggctt tatactcccc
     9361 ttttagatac tcctccaaaa aggtatttaa aggctgatgt gaatgaagat gggaatgata
     9421 tattttatag gaagtcggta aatgaattag gggtaggtgt aagtactatc cctaactaca
     9481 tctattttgg agcaggaaat gggagattga taaaagatat ttcacttact ccttatttat
     9541 attattcaga tactgagacg gcaacggtaa ttgtcgaaaa tgaggcggaa aaaattgaag
     9601 gatatgaaat tatcattgat gccctggggc agattaaaga agtgaattat tataaagaat
     9661 aagaaaaaaa tgaaaaaaat attaattaaa tttaaattaa ttgaagattg tcgattgatg
     9721 attgaagatt attcaaaagg tcagggaata gtggaatcgg tggtggcggt gggaatttta
     9781 atgatggtac tgacagggac agtgctcttg gtcagttttg gactctcaaa tcgaaaaact
     9841 ggttttgacc ggcgcaaggc caatgagttg gcgactttgg tgatggagga acagacgggt
     9901 agaagtatta gtagtccgga ggaattttgg caaaatacta attattccac tgatcctgaa
     9961 acaaaaccag gttatgaggg ttatagttat acgataaatc tatctgaaaa tgatgcctcc
    10021 ggctgtgatc cgacaataaa tgactgtgct ctggtggtag taaatattgg ctggtcggga
    10081 aaagagactc aaaatttaaa atatgaaaaa tttttttata aaaaataaaa tgaaaaataa
    10141 agccgccttt actctaattg aaattttggt ggtggtcggg atattgaccg tactagtagt
    10201 aagtgtgggg ggaattatgg ggactacttt taaagccaaa aataccagcg agggaaacga
    10261 acttttgtct tcaaaagcgg tttatatttt agatcagtta aaaaaggata ttttgagtgc
    10321 taacccaaga gaatttactt gtacggatgg tgttggttcg gtcaaaattt cttttaaaac
    10381 aaaaaataat acgacaacga ttctaacttg ttttgatgca gatgagaaga ttacttcgca
    10441 gacggcaagt ggaacttata agtatcttga ggatgatttt ttagtttggt gtaatggttt
    10501 tgtaacctgt agaaaagatg agacaaataa agtaacaaag gtagagctta aactaaatat
    10561 tggaataacg gcaaatgtgg ctggggcggg tagcactggg gtttattacg gtgtagcggt
    10621 gccgagagaa taagtgggag aaaaagagaa aaaatctaca atctacaatt tcaaaaataa
    10681 tcggaaaata atcaaaatgg ggttgtaggg gcttaattta ttaagcccta cgtgttataa
    10741 tcccttagtt agttatcata ttataggttt taaaattaaa atgactccag aaaaaaagag
    10801 tagtgttaaa aatgaagtta ataaggcaaa atcgaaagtt gccaagaatt tatttcaagt
    10861 cggtctggcg acatcgatgt tatttggagc tcctgcttgt gtccctacag aggcaatttc
    10921 tattattccg acgggcgaaa ccaatcccct accagacgat gctactaaaa ttcccgagtc
    10981 accgacagaa gcaaattatg aattaacccc gacggtaaaa aatccggcga cagaggaatt
    11041 gactttgacc cccgagccaa cggcgacagc gaccgaaatg ccaacagggc ttataaaaat
    11101 tgatatggac aatgtaatat atcccccggt gggttatgtt aaggaatatt ttgagaaagg
    11161 tgaatatgat gctggattag agacgataaa acaatgggtt ggcgtctggg aaaaaatggg
    11221 agtatttgaa ggattggaaa ttgagaacaa tagtttaagt ccagtgcctc ttgacggaag
    11281 agcaagaatt gtttgcgtta gagcagatga agaggcgcca cttctttgtc ccccacttga
    11341 tttgattaat ggtggattaa aagcagtgcc agaagaggga aattgggatg aaaccgatat
    11401 gccattaatg gtttcactgg aacgactgga agagttaatt agcagaggaa gcgaaactga
    11461 tatggcttat cagtttattg ataagtatca aaaatattcg atcaaatata ttgatcccaa
    11521 gactggtcag atggtggaag gttcctatat tccaccactt gaggttaaga aaaatgaacc
    11581 aatagagggt agcgtggttt gctttgctga tgaattttgt atgggagggc aaatgaaaat
    11641 agatcaagaa atggccgtaa agtattatga ccgttttata aatgctttgg ttaataatat
    11701 ggagaacaga gaatattttg atgtactgtt gtcaggtaac atatcacaag aaagtttaaa
    11761 gaagtatcta gtagaatctg gcggaatagt acctcccgga ttaaagctta tgcgtgattc
    11821 tggtggtggt tattttacaa tgagttatgt gttagataga gaaataaaat tgaatgcttt
    11881 aaagatgatt acttttgggc caacagaatg gaaaaacaac gtagggaata ttcaggagta
    11941 tataagtagc gtagacaatg taggtttggc tgatcctttt gttggtgact tgggtgttag
    12001 tctggttttt ctgggctggt atatagatag ctcaagtaat atcgtattgg ttagtggatc
    12061 aaaggatttt gaatctaatt ataatgacgg gagagttgag gatccctttc ccgtaatagg
    12121 gggaaaggat ggaaagtata taccggaaag agataatatg atagcaacag caacattttt
    12181 gtcttgggca tgtttattcg agaattcaca aaaaagtaga ccgttttttg ttgttccatt
    12241 aatggatata cctggtcaac ctgtggtatt ggatcaagac gttgtaaatt tttccggcaa
    12301 tcggactctt tttgtcaaat agtttctatt gaaagttgat tggatgtatg tcaaactagt
    12361 cgtagtgcga aaaatattgt tttttttagt ttcagttttg gttttgttat ttattttcgt
    12421 tttttgttgt tttaggatta aattattatg gttgggctct gatgataaaa gaatagtgtt
    12481 gttttctcag agaaaattat cgaactatat aaacgagatt tctaataaaa ataaatattc
    12541 tataggttta ttaagtttta gatctaagca aattttgaac aaccccactc tttttgagag
    12601 aaatgagaag ggtgaaatta tttatcagtg tggctggagt gagaagaata tttttggatt
    12661 taaaatttac aatttaaaag aatattataa cattgaagaa ttgatatcca aatatgaatc
    12721 aaaagaagaa atcgaacgac gtattaatga aactactgtg gcctgtttga ctaaattgta
    12781 tagtgagaat ttgaattttt tagattatga aaataaatat ttgaaaaata tagaattatt
    12841 actaaataac aatgagaata tatttttatt tgaatagaat aatatctttt tccttattta
    12901 tttttctatt ttttttatca gtaaataaag taaaaggagc ttatgtctgt acgggtcatt
    12961 atatgtgtgc tcgatatagc gttgcactta aactgtgtga caattctgac atactttaca
    13021 cagaaggatg tcctggaaca aacgaagctg attgtgaggg aggggttggg tatagctttt
    13081 gtattgggca gtgttatgtt aacgaaacat gttcttggga ttatgaggcc gatccttgta
    13141 cttgttcatg ggggtcttat ggtagctgtg atgggaattc gactaatacg gcgtgcataa
    13201 agtatagaac atgtacttgt gatcctgttg gttgtggttc atgtactggt agtaattgga
    13261 ctacgtgtac ggcaagtgaa ttgggatgta ctgttcaaag ttgtccaaca tatgagggtt
    13321 atacaggtgg gagtgtgcgt gatgggacat taaatggtga ttgtacctgt ggaatgaaaa
    13381 cttgtccgcc tatttgtacg gcgacgaatc cggatgcacc ggtgttatca aatcctgctt
    13441 ctggaacaat agttaatgtg aatacatctg taaatttgga ttggaatgcg atttcaagct
    13501 ggggtacagg ttgtccccag agtaataaat atcaggtttg tgtgatgtcg gatagcactt
    13561 gtgatttact aaactgggtt gatgtggctt caactgagac tattaaagcc tggacaccaa
    13621 caagtgctga ttctttggta acttggaaag ttagaagcaa taatgggagt aatacggcag
    13681 agtctttgac aagaacttta cgagttaatg caccgccgac attaaatttg tcaccaacaa
    13741 attatttggt attacaaaat tcagttgcga cagtagtggc tccggaaact ggaaatagaa
    13801 atcatatttg tcaaacaata tttactaatc ctccggcgac agccagacaa attaaactgg
    13861 tggtgacggc gactgatgtg gatggaggta gtgatattgc cagtgtttcg gtgagattga
    13921 atgcttcgac aacagttaat tcagcgggaa cggtgactgg taactggtct ttagtgtctc
    13981 cggttactag cagtgttggt ggaaacaata aaatttatac ttttcatatg agtttaggaa
    14041 gtgggttaga tagcggtact ttatataatt tagaggcttt tgcaactgat atctggggag
    14101 cgacaagtgg atggatagat agtggtcgtg attttaaggt ttgggattgt caggtgctga
    14161 caagcggaac tttatttaaa gccactaatg cggttcagtc ttgcccaacg ataggattta
    14221 ctgatccaat cgatgttgga gttagtttta attctataat tttttctggt ccaccagata
    14281 taagtataac tgacgatgat ttagatagct atggtacaaa taatgttact tgggggaaaa
    14341 attatctacc gctaattaat ggaggtgatg tttctaatat cgatggagat ttgttagcta
    14401 caggcagagt aacgagaata attgatatag gaacgggaac gacgatttgt ccgggtgctt
    14461 cccaatttac tatcggaaat agtattatta atccatattc tctttctcct caagcgcagg
    14521 ttgatttttc ttttatacga gatcaggagg cgtggtatca ggtcgtggga gcaggagtaa
    14581 aatcgaggaa tcgattggag tatggagtgc cagtgacagc gccagtggta agtagatttt
    14641 tgacactttc tagtgtcaca aacggcaatg gtttagtgtc gggaatcgac tttgaaaata
    14701 aaaatgggaa taatttaaag aacgatattg gatcaccaaa taattggtat gtactgagaa
    14761 atactaatga tttggatatt tataattata attattttta taatgatttt tttgttaagg
    14821 cgggggtggg agttactggg acagattggg ctgaaaagcc ggaaaacgga atttatttgg
    14881 tgaaaaatag tttaaatatt acgagcgatt ttgtattagt cgatacaacg aaaacaatga
    14941 tggtgattgt gaatggggac atcacaattc aaccaaatgt aactcaactt gatggaattt
    15001 atgtggctga tggcagtata aatgtaggag gaatatcgga taatcagttg aatataaatg
    15061 gaatgcttta cgcgggagga agtgtcggat tgtatcggag ttttactgat aaaactgata
    15121 acaacacaac tccagccgta aaagtaaatt attcaccagg gttaatcttt aatttacctc
    15181 caaaaatact cagagtcttg tcggggtgga gagaggaatg atgaaatcaa tcttcaatta
    15241 tcaatcttca atctttaatc tacaatcaac aatctacaat ctacaatcta caatctacaa
    15301 tcaacaatct acaatcaaca atctacaatc tacaatctac aatctacaat tatagattat
    15361 gaaaaaatat aaaaatctaa aacttaaaaa agaaaattag tatgaaatta tttaagaaaa
    15421 attttattga agattggaga tcgaagattg aagactttcg aagtggtcag gtggcgataa
    15481 ttgtgatgct ggtctcgact ctattgatta ccgtcggctt gtctttatca aaaaaagcca
    15541 cagttgaaac aaaaattgac tataacgagg aagctttaaa aaaggctttt aatgcggcgg
    15601 aatcaggaat tgattattat ttggctacgg gtagaactga atatcaatca gatgataaat
    15661 tatctcaggc aaatttagtc agagaagaca tcgacgacga tggtactggt gtatttgatt
    15721 ttagagaatt tacctcggaa aatggaatac aatatctttg gcttgttgat catctggcag
    15781 atggtggtct cggaaataat tatttcgacg gaagtcaaat taccgtgatt catggatgga
    15841 cgagtaatta tggttcactg gaaataaata tattttataa gaatggtgca agttatgctg
    15901 taaagcgata tgggtacaat tttagtagtg atgttaatag acaagtaaca aattttagta
    15961 atgctattgg tcaatcaaac gtgacaattg gttctctccc aacagatcca attttgatga
    16021 caattactcc aatttttact ggagctaatc tgtcagtgag cggtaacgga tctgtttttc
    16081 ctgttcaagg agagagaatt aattcgacgg ggtcggcagg agtggtattt ggtgagacgg
    16141 gggaagaatc gaaaattaat aaaaaattaa gtattgaaag gcgctataaa ttcccctttt
    16201 tcctcctctc ggggatagta tcggaaactt cggtcttgag tcaataagaa gaattgtata
    16261 tatttatata atgaaaagta tcgcgggaat tgatatgggc cttggcagta ttaaggtggt
    16321 agctttggat cgggtgggag aagtaatgtc gctgacggct atcggagagg taaaaactcc
    16381 aaaccaggat tggatgaaaa gtgaagtgac taaaaaaaat ttagaagagg tggctaagtc
    16441 tttaaaaagt ttaatcaatg atttgaaaat tggaaatcgg gcggctgtag tggctttacc
    16501 agaatatgaa attgtcagtc gtctggtgtc actacccccc ctggaagaaa aagaaataaa
    16561 agatgccctg acctttgaag ccgagacctt tgttccctac ccattggacc aagtctctat
    16621 cgattatgag gtaattgagg aggatgaagg agggcgcctg acggtgtttg cgattgcggc
    16681 tcgaaatgat ttgatagcta actatataaa aatatttaaa gcggtgggga tggagctgct
    16741 ggccattgaa tcccccgccg tgtcgatgag aagaatttta aaacaaattg cgacggcctc
    16801 tgaggcgacc atggttctgg acattggtca aaaatattcg gatttaattg gatttaaaaa
    16861 tggaaacatc tattttgcca gatctatttc tttgggaggt gagtcactga ctcgggctat
    16921 ttccgtaaat ttgggtcttg atatggctag tgcggaggaa tataaaaagg cctatggaat
    16981 aaaagagatg gaacttgagg gtaaaattaa aagcgcaatt atgccggttt ttgatagtat
    17041 ggcggaagaa attaggcggg cgatggctct ttatagcgaa ggtaataatc gggcgattga
    17101 gcttttagtt ttaagtggtg gtggagccaa actaccaggc ctggccgaag aatttactcg
    17161 aattttggga attgaagttc aggttattca gccttttttg aaaattgaca gtcaaaaaat
    17221 tgtgtcaccc ataaatttag aaattgatgg ctatcgcttt tcgacagccg tgggactagc
    17281 tctccgggag ggacaatgaa agttggtgat agcttaaata ttaatttact gccaagtcag
    17341 gctaagtttc aggcggataa aattaagcta aaaaaaacta ttaggcgcta cgaaatgagg
    17401 tttctcggcg tctggctggt atttttaatt gccactttta ttttattttt ggggagtgga
    17461 attatattga gtgggagtca aaaaaagtac cagcaggcgg ttaatgtttt tgaaagtaat
    17521 actgaagaaa ttgttttaaa tcaattatta aaatatcaag ccaaggctct tgggcaggta
    17581 ctaaatgatc gttttgagta cgcggcctct tttgaaaaag tcgcctcgat tttttctgac
    17641 aaggcaaagg tatctgaatt tgaaatcaat gacaaggata aaagttttac catgacagta
    17701 actacggctg ataaggaagg agttgattat gttgaggata gagttgatga ggttaatgag
    17761 ggtaaggttg aaggggtaaa aaaaatgata attaccggag tttcttatca agttagagga
    17821 gagtggtcta ttaatttgga ggtaatgata tgagtaagac aatggaagta tcggataaga
    17881 tttttggcat ggataagcga atattttctt tgtggcttga accctttgct ttatttttag
    17941 tcttttttat gagtatattt ttggtgatag tgccaaaaat taatgaaggg atagcgaggc
    18001 ttaatgaaaa taagtctgta ttgcaaaaaa cggcgcaagt gaacgaaaaa attaattatt
    18061 tacaaacaat agatcaggaa ggaattaaaa aagatgccca gaagttggcc caaggtttgc
    18121 tcccagaaaa atcttcatat ttgttgttaa aagtgattca aaatacggct caaaaattaa
    18181 attataatat tgacgatttt tcgattagta tgggtgatat taaagatgaa acagcagaaa
    18241 atattaaaga ggcgagtttt gataaaattc cgattgaaat aaatttaatt ggtccgtctg
    18301 aaaattattt ggctctggcc aaagccctag aaagaagttt gccgataatg tcgataaata
    18361 aatatgaaat gtcgtcaaag gcgggtggaa taacgactat caaattggcg gtttatgcct
    18421 attatatgaa ggaggtaact aattttaagc tggaaaatat cagtctggct gatttgactt
    18481 taagtcaggc tgaagcagat ttattatcga ctattggtga atatagtgaa ctggatgtca
    18541 agagtattga aggagtagga ggaagtggga ctttcgtaaa atacgataga attgacccgt
    18601 tttttactcc ttaatttgga gtaaataacg aatttcagga aaagccaaat taaggagggt
    18661 gcggatagat ttgatgattt tgccttcttt accaataatt tgaccaacaa tttccggagg
    18721 ggcactaacc tcgagaatgg taagttcgtt ttcaagacga gaagaaacct gaacctcgtc
    18781 gctgtcagaa acaacggctt tgattatata ctcagcggcc tgtttaatat cagtcatatt
    18841 tattttactt cttctccgcc agaggcggat ccgccttcgg cggaagtttt ttcaacaggt
    18901 aattctttta tttcttcagt ttttattttc ttctttcttg gatatttgtc aggatttaga
    18961 agtttatcaa caccagtggt gatttgggca ccatttttta gccactgagc cattttatct
    19021 ttatcgattt ggagagtatt ggtagtcgga gtgtaaaaac cgagatcatc gataaattta
    19081 ccattagctt tacttcggtc tggggcaacg acaatccgaa atgagcgttg atgaatttta
    19141 ccagtaggtt taagttttat ttttagcatg gattcgatta tacaataaaa aaagtctaaa
    19201 gtctaaagcc aaaagtctaa aaaataataa aaaaaattct aaacactaaa tcctaaagtc
    19261 taaaatttaa atttaatttt tatttaaaat aagaattgcg cgagtggcgg cgtcttcctc
    19321 agctttttgt ttggaattac ccttacccga agcgataagc tgatcaccta aataaacacc
    19381 gattttaaaa attttttgat gatcaggtcc ggactcagaa agcaattgat attttggagt
    19441 aactcccctt ttggactgag caatttcttg aaagaggctt ttggcgtctt tatagatgtt
    19501 tttttcagat aatgcctgaa tatttttatc taaaaatcga tctaaaaatt tttctacagc
    19561 cggatagccc tgatctaaat gaatggctcc gataagactc tcaaaggtgt cggccagaag
    19621 agatatattt tgacggccat tgtgagtggc ctcaccccgg ctgagtaaaa taaaggagcc
    19681 gagattgaag gaggcggaaa tatcggctaa attttcagtg cagacgatta aagaacggag
    19741 attggtgagc tggccttcgt caaagtcggg aaaaagagaa taaagttttt gggaaaccca
    19801 gtattcgagg atggcatcgc ccagaaattc aactctttcg ttgctcgagt ttaactgttt
    19861 attttcatta agataagagc ggtgggttag agctagtaaa agatagcggg gatctttaaa
    19921 tttaacttta attttttgtt ctaaaagatt tattttatcc atttattttt tttaagatat
    19981 taccgagaac ggcgttgata aaaggacttg atttggtatc gccaaattct ttaccaattt
    20041 caatagcttc atcaataatg actttagccg gggtctccgg tttatttatt agttcccaaa
    20101 tagaatagcg aagaatagcc agttcgacct tgttaatttg atcaagaggc catttaggag
    20161 cggcagtcga gattaactgg tcaattttat taatttcaga gatgatgggg agaagactat
    20221 catcatctat cttatgggta atcccccagg caaaaagtga ctggatggat ttgattcttt
    20281 tttgatgtcg aggatcgttt ttgcttttca cttattgcag tttttgcagg taaaacccgg
    20341 aatttttggt tgtccacaat ttttgcagag ggtaacctgt gatagggtgg tggctttaaa
    20401 tgaagctcgg cgtttgccct gacgacggct tgagggccag tgtttaggaa gtgcagtcat
    20461 ggaggtatta taacagagaa aagagggaaa ttttcaatct acaatcttca attttcaatt
    20521 taaattaacg ggg
//
LOCUS       DHGB01000023            7508 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_55624, whole genome shotgun sequence.
ACCESSION   DHGB01000023 DHGB01000000
VERSION     DHGB01000023.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_55624"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    918..961
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2102 a         1566 c         1291 g         2503 t
ORIGIN      
        1 agtctttgtc acccgcaatt tattacgtgt tctcgcccgt aaaaataata attaactatt
       61 taaccattaa tctattaatc tactaatcta ataatctaaa ccatgaatat tatgaaattt
      121 aggccactat ttttgtttat ttcttctctt ttgattttag cctccatctt ttctattatc
      181 ttttggggct ttcgtctttc tgtcgatttt tccggtggtt cttcctggca ggtttctctg
      241 ccttcgaggc caagccccga aacccttaaa actatttttt ccaaaaataa tttagaatta
      301 ttgactattt ctacaaatca agataaatat atccttaaat ttagccatat ttcagcagag
      361 caaaaagaag ccttaagtat cgaactaaaa aacttagatt ccaattttag tgaattgcag
      421 ttcgaaaccc ttggtgctac tctcggcaaa gagcttctta aaaaaacaat ttgggccatt
      481 attttatctt ccctttcttt attgttgttt attggttctc gatttaaaga tattagtttt
      541 ggggcttcgg cggtcatcgc catgcttcac gatgctttaa ttcttcttgg ttctttttcg
      601 attttaggtc acttttttgg tgccgaaatt gacgctcttt ttgtgaccgc tattttaact
      661 accttatcct cttcggttca tgacactatc gttacctttg atcggattag ggaactcaaa
      721 agcctcagct ttcatttgga ctgggtggag ctagccaaca aggctgtctc agaggtcatt
      781 gtccgctcga taaacaattc tatgactatt atcttaatgc tacttgctct ggttattctt
      841 ggaggtgcca gtacccgttg gtttgccatt gctctgctca tcggtgttgt ctctggtacc
      901 tattcctctg ttggggtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 ntcggcaaaa agtttatcga gcacttcgcc tacttttcga cttggtttta tcttcaatat
     1021 ttccatcacg tctagcccat taactttcag gtctttaact gaaaaaggtt gtttttggac
     1081 ttctatcagt cgctttttaa ataattccca tcgccagctg ctttccttag ctcccgatcc
     1141 gagtctatcg ctccgtcgca gtgaaatcat ttcgtctaaa tattttggcg tcacatttct
     1201 gataaatctt ctgacggctt tatcagtttg agtatcctcg acggtaaaca tgtgccatct
     1261 gaccaaaata aaaagctgtt ccagttcatc cttcgacagt cttaatcttt tcccgatatt
     1321 gaccgcaatt ctggatccaa ctacttcgtg attgtaaaaa gttttttctc ccttttcttc
     1381 tttcatgacc accggttttc ccacatcatg aagtaaacag gccagtctcg tcactggatt
     1441 tttactttga cagttattta aagcctcaag ctcatggtcc caaacatcat aaatgtgatg
     1501 acctttttgg cccataccca ccccgagtag cagctcgggc atgatttctg ccagcaatcc
     1561 cgagtttttt aagagtctta ttccatctcc gggttttttt gaaagtaaga ttttaaataa
     1621 ttcgtctcta attctttctc cggctatttc ttttattaaa tgagcatttt tttgaattga
     1681 ttcaaaagtc ttttcttcaa tcaaaaaacc aatttgggcg gcaattctaa tcgctctcat
     1741 cattcgcaga gcgtcttcag aaaatcgatc atcggggttt cccacagttt ttatcagccg
     1801 gttatttaaa tcttcaattc ctccgtgcag atcagtaatt tgtccttcaa tatcaagggc
     1861 tatggcgtta atcgtaaaat ctcttctttc cagatcttct tttaatgttt ttccccagct
     1921 aattttatcc gggtgtcttt tgtcactata tcccgcctca cttcgatagg tggtaatttc
     1981 aaatatctcg tttttgccaa ctaccacact aaaagtccca aattgatttt cgcaaaaaga
     2041 atttttggga aaaattttct tcatctcttc cggggtcaaa ttagtggcaa aatcccaatc
     2101 attaactttc ttttttaaaa tccaatcccg aaccgctccc ccaactacat aaatttttcc
     2161 gtttttatcc aggacttttt gcatcactat tttaaccttc tccggtaatt gtcttttctc
     2221 catatccaat tttaccttaa actgagacat tttttctcca ccgaaaaccc cccgccgctg
     2281 cggggggttt aaagatcaaa taataaatat tatttacatt tggggtcttt caggacgagg
     2341 taatgcctct tttacggcga tatttctacc gtcttgctcg ctgccatcca aagatagagc
     2401 ggcttgagcc atttcctcag tttcgaattc tacgaaaccg aaacctttgc ttcggtgaga
     2461 gaatttgtcg aaaactatat ttgcggagat gattgtgccg aagggtgcga agatcgcatt
     2521 cagctgctca tctgtcatgt tgtaggaaag atttcctacg aaaagctttt ttgccatttt
     2581 tttcgaatct tttaaagatt ccttaacctt actaataact aaccaaaaac taggtaagtg
     2641 ctttctggtc ttcaaattat agcatacaat aggcctatgc aagcacttct taatttgtta
     2701 agtcaagaga caataagtgg ccaaacaccg ccaaaaactc tcttaaaact cctcctaaaa
     2761 aaccgccaga ttaccgacgt ggacaacttt ttaaaaccat cttttcccga gtcaaaacta
     2821 tcatcaaaaa aagtcgttaa cttaattcaa aaacatatag ataaacaaaa aaatatctta
     2881 atttatggtg attatgacgt tgatggctta acttctacgg ccattctgtg gcaaagtctt
     2941 tatcccttaa acaaaaatgt cttgcctttt attccccacc gggaaaaaga tggttatggt
     3001 tttaaatcca aatcattttt tcgccttgaa tcagaaaaaa aaattaaatt tgatctgtta
     3061 attaccgtcg ataacggcat tgtcgccgat gctgagtttg ccaaaattaa agccaaaaat
     3121 cctaacacaa aaattgtggt gattgatcat catcttgcnn ttagcctggt taattgccag
     3181 atccttttcc aaaacagctg atttgtcgct ggcggccctt ggagtcgttg ctgattgcca
     3241 gccactttcc gatctaaatc gttcgattgt tgttcatggt ttgcaggaat taaaggtgaa
     3301 cccatcgccg ggtctcaaaa aattaatgga aatttccgga gtaaatctgg ataaattatc
     3361 ggtttatgat ttaggttttc ttttagggcc tcgccttaac gccgttggcc gtctctctga
     3421 tcccaccgac gcccttcgac ttttgtgtag ccgaaattct ttagaagcag gtaaatatgc
     3481 ggaggtttta aataatttta ataaagaacg acaagattta caaaaagaat ctatcgatct
     3541 tgccgataaa aatattaata taaaaaataa agttattttt attgctgatt catttaatcc
     3601 cgggattatc ggtcttattg ccggccgtct gactgaaaaa tatgctctac cttcaataat
     3661 tattgccaaa aatggtgagg tggctaaggg ttcctgtcgc tcgattccgg aggttaatat
     3721 tatcgaggtc ctccgaaaat tttctgaatt atttgttgat ctcggtggtc accccggcgc
     3781 cgccggtttt tctattttgg ataaaaatat tcccaaactt caaaaaaaac tattaaacta
     3841 cttttctatt aatctactta actattcccc ccaaaagtcc cttactattg acgctaaaat
     3901 ggatctttca gccattaatt taaccaatat taaattaata aaaagtttgc agccttttgg
     3961 tattggcaat ccgcagcctc tgtttttatt tgaaaattta aaaatagagt caaaaaaaat
     4021 ccttggccaa actcaatctc acttaaaatt aaaagtgtcg gggatagatg ccatcgcttt
     4081 taaaaaagct gatttagata tagatttaaa aataggggat tcaattaatc tcgtcgcttc
     4141 cctcgacgcc aatacttgga acaatcacac cttgccccag ttagtggtaa aggaaataat
     4201 tacgcggtaa aattacccca tgaccagaac cttaattaaa gatactattt ccgaaattgg
     4261 taaccaagtt attattaaag gctgggtaaa ttctgtccgt agctacggca aattagcctt
     4321 tgctgatatt cgcgatcgct cagggcttat tcaggtagtt ggaggtaaag atttaggtga
     4381 aattagagtt gagtcagtgg tggaaattac cggcaatatc cgtgcccgcg aggagaaata
     4441 ttttaaccct aaaattatta ccggtaaaat cgaaatggaa atagttgaat tgaaagtgat
     4501 cgaagcagcg gctgaattac cttttgatat tcatcagccg gatttgaatg tttccctgcc
     4561 cgttttactt gacaatcggg cggtctctct tcgtcatcaa aaagtgaccg atgtttttaa
     4621 agttcaggcc accattatga aagcttttcg tgatcattta attcaaaatg attttaccga
     4681 aatttcagtt cccaccattg ttaccggttc gaccgaggga gggtctgaag tttttccggt
     4741 caattatttt ggtcataaag cctttctggc tcaaagtccc cagctctaca aacaaattat
     4801 ggtttctatt tttgaaagag ttttcacttt ggctcatgct tatcgggctg agccctccgt
     4861 taccacccgt catctgaccg aatatcttag ccttgactgt gaaatgggtt ttcttgaaga
     4921 ctggacggat ttaatcaaaa tggcggattc aaccgttaaa gccatcttta aagctgtttc
     4981 cgaaaaacac caggacattc ttgataactt tggtgttact atcccaaaga cctgtgatga
     5041 caccccctgc ttaaaattaa aagaagctct tcaaattatt tatgagcgca ctggtcgcga
     5101 tgttcgaaac gaactggata tggatcctga gggggaacgg gaaatttgcc gctggtcgat
     5161 ggagaaatac ggcagtgaga tagtttttat tacccatttt tacacccaaa aaagagcttg
     5221 gtattctttt gctgatcctc aaaatcccga ggaaactttg acgatcgatt taattggtcg
     5281 aggagttgag tggatttccg gcggtcaaag aattagtaat taccagcagc ttcgggaaaa
     5341 attaaaatcc atcggtcaaa atccggacga ctttgaaata ccctatggtc aggcctttaa
     5401 atttagtatg cccgccgaag gtggttttgc catcggtctt gagcggatta ctcaaaatat
     5461 tttaggttta gaaaatatcc gcgaggcttc tctttttccc cgcgatatgg aaagagtcga
     5521 tcagagatta cctaaaattg ataaaaaaat aaaaaaataa attgaaccaa gtgaatttat
     5581 caaaactgct gcggaatcct tattttcact atggtctatt tttaggtctg gctttgatct
     5641 taacttttct ccccacttca tttcgcttta ccaaaaaaaa tgatcttttt acctttaaac
     5701 acccgcccca gccagtcgtt gttaataaaa ataaacctca gctttccaca aatttttata
     5761 ttctgattga taatgaaacg aatcaaatcc tagtttctga aaatgaaaat actcgcatct
     5821 atcccgcctc gacgaccaag cttgtcaccg ctctcaccgc tttgaatatt taccctcttg
     5881 aagaagtcgt caccatttca aaggattatg ttgacggcaa agtaatggaa ttaaaatccg
     5941 gtgataaagt tactatcaaa acattagtag ctggtcttct cattcattcg gcgaatgatg
     6001 ccgctttttc cttagcttct cattacccgc agggaattac cggttttgtt gatcagatga
     6061 atttactaac caaaaaatat ggtcttaaaa atacccattt tgtaaatttt gacggtcttc
     6121 atagtcccga tcattattct actgtctatg atttgagtca gatcgcccgt atcgctattc
     6181 aaaactctac tttacgaaat tatgtcaaaa tcaaatcaat ggaattaacc gatatttacg
     6241 gccaaaaagt atataaagtc cttagtacta acgagctttt aggagttctt cccgaagttg
     6301 agggtttgaa aaccggctgg actcctgagg ctagcggttc ttttatcgct ctattaaata
     6361 ttgacggcca ctatcttatt tctgtagtag ctcaaagtga ggaccgcttt aatgatacta
     6421 aaatactttt aaactgggct aaagaaaatc ttacttggcg tatgtatcag taactgctcg
     6481 cacataggct acaaagccac catcaccctc aaggacataa acaggttgca taaattgggt
     6541 taaattaacc ggttcaaaat aagctaaata aatttttctg ataatcactt tatttgaact
     6601 gctatccttg gctggccagt aattacccat ttttaaattt tcaaatgctt ctttgctggt
     6661 aataatggga taggttgagg aagaagcttc cgatgtatcg acatttacat gttgataatt
     6721 tacttcgact acttttttgg ccgcgacact tgagccggat actagcaccg aaaccggtgc
     6781 tttacctact tccgccgtta ctacctgaaa atcctctgcc aatttttttc ggaaaaaatc
     6841 tactctaata atgttggctt ctgaaagagc ggttacctct tttaggccat caaaatttat
     6901 tttccaaaaa cttacctttt tttctccttc tgctaaatca ggactcattt ttcccgcttg
     6961 ttcgagaaag tcagaggcag tgataatcgc ttgttcctta tttggcattt gctccgggtt
     7021 aagtaataat tgatccgtca aatagggata actcatttta aagctacttt ggagaacatt
     7081 catcgttaga cttctgccgg tatttggatc gttgaaaaca tatattccca cacctgtttc
     7141 tttaggttcg gtgagaaagt tcatagctgc cgctgttttt ttaccttctt caagggcgcc
     7201 aataatactt ttggagcgat aaattacata gattttggcc tggtctttat atttcggaaa
     7261 agtatcgtcc ggtaattcag ccgtaaattc ttttttttgg aattcttttt ctgggaaaaa
     7321 cactttatct atcaccccgt atctgactgt cggtgctaca taaggtggat tggctttttg
     7381 ataagctttt atcgccgctc cgattcccca ccaaaaaaat aaaaatccca cgaggcctat
     7441 tcctccgtat ttaattacat ttctactggt ataagcaacc tgagttaaag acattaaatt
     7501 attttacc
//
LOCUS       DHGB01000024           14540 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_19825, whole genome shotgun sequence.
ACCESSION   DHGB01000024 DHGB01000000
VERSION     DHGB01000024.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 14540)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14540)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14540
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_19825"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    12180..12200
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4746 a         2814 c         2045 g         4912 t
ORIGIN      
        1 tccgtcacca tggccacttt taaactctct tttaacatct taataacttg gggaattttt
       61 tgtctttcaa cttcatcata aaaagaaact aatttaggct tattttttat ttttaaattt
      121 gttaataaaa ttcccgtttt tctagtgtct tcacataaaa gaatatcgat actttcaaac
      181 gtatcgatag ctctcaatgt aatatctttc atattgccaa ttggtgttgc gactatataa
      241 agcatattta gttattaaat tgtctaatta ccgccactac ctttccccga atctcaacat
      301 cagttacata catcgggctc attgtcgaat tcgccggttg taaacgaact cgagtcgctt
      361 cttgataata tctttttaag gtcgcaaaac cattttttaa taccgccacc acaatatcac
      421 cgttattggc tattttttgt tcttttacca ccacatagtc accgctcaaa ataccatcat
      481 ctatcatcga atctcctttg gcctgcaaaa caaaagattt atctccagct ttaattaaag
      541 ttgacgagac cattaaagtc gcatttggat caggataagg ctcaatgggt tccccacagg
      601 caattaaacc caacactggc acttcaatat tgtttgctaa gtttttatct tcttcctttt
      661 taagcagctc tattcctctg actgctcctc gatagcgtct aattaaacct tttttttcca
      721 gagctgaaag atgttcatga accgtagcta gtgatgataa gcccatagac tgggctattt
      781 cagtcaaggt gggcgcatag ccgtacttat cgattgattt tccaattaaa tccaaaattt
      841 ccttttgtct tttgtaaatt atggctggca taacctcatc atacacgaat aatacccgaa
      901 gtcaatcatt aaattaatga taagattaaa ttatgttttt tgccattata tttctattct
      961 ctattttttc ctttctttta acacccgttt gtcaagccac tccggaattc acagttaccg
     1021 aaaaaataga atatctcttt tctttttcag gtcaggctac tgtcactcac caaataactt
     1081 taaccaataa tttttctcaa atttatccca ccgaatacca aattcaaatt cagggtctac
     1141 ctctcagtga tctgagtgct agtgatgagc agggaaatat tctaagtgac ttttctcatc
     1201 aaaatgatct tagtaccatc aaactaaaat ttaatgaggc gaaagttggc aaaaatcaaa
     1261 gtaccagctt taaactaatt tatcgtattg ccaatttagc caaaaataag ggtaaaacct
     1321 gggaagtaac tctcccagag acggttgaag atgtcccaac aagtagtgaa attagcctaa
     1381 aaactccatc ccagtttggg agtctctctt ttgcctccgt caatgtcaaa tttgaagatg
     1441 gtttaactca aaatatcacc actttcaata ataattctgg ttcaaaaata ttaattatct
     1501 ttggtaatta tcagcttttt gattttactc taaattacca tttaagaaat cccaattcag
     1561 ctaaagtttc ttctgaaatc gctcttattc ccgatactta ttcccaatca gtttattatc
     1621 agtctatttc acctcctcct aaaagcatca atattgatga ggatggcaac tggttagccc
     1681 agtatgaaat taatcccggc gaggaaatag acattatcgc cgtaggtcag gttaaaactg
     1741 gaattaattt gccccaagcg atcaagaacc tagaaaatta tcttcttccc caaaaattct
     1801 ggccaattga cgatcctcaa attcaaactc ttttaaacaa agtaaagact ccacgctcaa
     1861 tttatgacta tgttatttcc actctcaact acgattttag tcgcgtaagt aatgccagtc
     1921 gtcttggagc tattgaggct ttaaacaacc cccaaagtgc cctctgtact gaatttactg
     1981 acctctttgt cactctggct cgggctagtg gaattccagc gagggaaatt caaggttttg
     2041 cctattcaac tgatcaaaaa ataaaaccgc ttagtttaaa taacgacatt cttcatgctt
     2101 ggcctgagta ttacgattca aactcaaaat catggaaagc cactgaccct acttggggta
     2161 aaactactag tggtatcgat tatttcaata atttagattt aaatcacatt acctttgtca
     2221 cccatggtct taatagtgaa taccccctcc cggctggatc ctaccaaaaa gaccaaaaca
     2281 ccaaatcagt ttatattgat tttgccaaag aggacttaaa atatgaacaa acatttccct
     2341 cgctctacct taaaaaaaac aagctcatcc tccaaaatcg gtcacctaat tcccaaaaaa
     2401 atatctctat ttcttctgat aaaatttctt tccttaaaga aattgataaa atcttgcctt
     2461 attcttcttt agaaatcgaa cttcccaaaa tttcattctt aaaatcactt tccaccaaaa
     2521 atcaaaaaat aacttttact ttgacaagct tagaaggtca aactcaaact caaagtttga
     2581 attaccctcc tcattttata aatctgggca tatttatcgt tttaagcctt ataattttat
     2641 cgataagcgg tataataatt accaatctga ctcatgaaaa aaatacttaa atacacttta
     2701 tttttagctt tcgctctagt tattgaaaat gaaatttggg caaatttaag cttctcactc
     2761 ggaatcgaaa ccattattaa agtcgctcta attctggcca tttttgaatt aattctcaaa
     2821 ccgattctaa aaatattatt attaccggtt acttttttca ctttaggttt aattcgaatt
     2881 gtaattaata ctctcggtct ttatttagct gtttttctga tctcagattt taaagtaaac
     2941 tctctccaac tttttagtta tcctctaagc ggctttctcg cctatctttt tacttcaata
     3001 attgttagta ctctgcttta tatttttaaa aaattatgaa cagcgctctg accattattc
     3061 aaataattct ttgtctaatt ttatccggac taatctttat gcaatcaaaa ggaagtaatg
     3121 aaaacaacaa tattctttcc gaaactgtct ctcaacgtcg tggctgggaa aaaattcttt
     3181 ttaacttaac cctatttacc attctcttat ttttaatctc ttcaatcatc caatctttga
     3241 tttaaatgct cacgaaatta cgctttaagc tcgaattatt atttctctat ttcaagagaa
     3301 gttggctata ttttctcatt agtcttctga tcggcagttc attaattatt tttcgccaaa
     3361 atatagttag tctctataaa aaagttaatc acccgactca aaaaataggc attgaagggc
     3421 tctttactag tagcaattta ccaccagaaa ttagccaaaa aatatcttac ggactaacca
     3481 taaatactga cagcaataaa tttgacatct cacctctagt aaaaagtctc gacatccaaa
     3541 atgatcaaac ccaatatttt tttgaattaa atcctgattt caagtggcac agtaaacaat
     3601 cactcaaatc ttctgatttt aattacaaaa tcgctggtct aaatatctct ttcccggatt
     3661 caaatcatct gattattcaa accgaaaagc cctttgctcc tattttttcc actctcagtg
     3721 agcctctaat caggaaaaat tttgatggcc tgggaaaata taaagtagac agtttccagt
     3781 atcaagacgg ctatttaaaa aatttatttc tcaaatcaga aaacagtaat ctactttacc
     3841 gtttttacca aaatgaaact gatctgctaa atgccttcaa aattggagag gtcgatcaaa
     3901 ttgaaattag ctcggtacct gaagacttaa atgattggcc taagctgaaa attaccaaaa
     3961 atatcaaaac tgacgaaaga tatctcgctc tttttattaa taccgaaaag cttaacagca
     4021 aacagtttag gcaggctcta gcctatgcta ctccaaaaac aaaagattta aatgaaagag
     4081 ccctttcacc aatttcaccc acatcttggg cttataatcc cgaagttaaa gaatatgctt
     4141 ttaatgagac tcgagctaaa gagctcatca aggacaacga gattgagtca ataaatcttt
     4201 cttataacga ccgacgatta attgacctgg ctgacagtat taaaaaaagc tgggaagaga
     4261 ttctcggaat taaagtaagc gctcaaatca gcaaccaaat tgacactcaa aattatgata
     4321 ttatcctagc ctacggcagt attttaaatg atcctgatca gtatttattt tggcactcga
     4381 ctcaaactaa aaccaatatt accaaaatca atcatcctcc catcgataaa ttactagagg
     4441 aggggagaca aacttttgat caacaagagc gcaaaaagat ttatctggaa tttcaaaaaa
     4501 tactactcga agaatcccca gctattttct taaaatatcc aatcctttat actatttcca
     4561 gaaattaaat tatatgaatc aaaataaaat cacccctttt ggctatttcc tcctactctc
     4621 tttttttgta cttctcagct atgccggctg gatttattct caaagtattg actgggatgt
     4681 tttaaaaaga atggaaaata gtcctttgac tctacccact caaactatta ttactccttc
     4741 tgctactatt tccgccaccc caactgttaa ggtaaactga gtcttatgga aaatgtcttt
     4801 cccatcttag aggtaaaagg taatcatcgc gatttaggct ataccatcgg ccaccgtttc
     4861 caaaaagaaa ttcaagatag aattagatct cgaaaagaaa atatccccga ttacaatgaa
     4921 tatttaaaaa aggttgacgc gtactatcag attactaaaa aatatttacc ggagattgtc
     4981 gaagagatag aaactatgtc cgagggatcc ggagttcccg ttaatgattt atttttccac
     5041 aactgccgcg agatttataa tttatttata aattctagcg aaaatgacca ctgtactact
     5101 gttgtttctc gaaataataa ttctgtcctg gtcgggcaca atgaagattg gattttagaa
     5161 gggatcgatg atttgtatgt attaaaagca accataggaa aaacaactat tttaggcctg
     5221 gccaataaca cccttctccc cggtgacgta gccatggcca ataatcacgg tctaatccag
     5281 tgcatcaatg aattaaattc agatagtggt cttggagtcc ctaaaaattt tgtggctagg
     5341 gctgttttag attgtgaaaa tattgacgag gctattaatt taattaaatc actatccctt
     5401 gcttcgggtt ttaatcatgt tctcgttcaa aaagaaaaaa tagtaaatat cgagatggcc
     5461 ccttcaaaaa tatctattaa caatatcaaa caaaatacct ttgttcacac caaccatttt
     5521 ctctcgtctg aattaaaaaa tatcgaaaca ttccgttcca aaagttccga aattagatat
     5581 cgaaaagcag ataaattatt aaataataaa aaacacaatt taaataatat tttttcttta
     5641 ctttcagata ccagtgataa ttgcgaatca atttgccgta atgaaaccat cgcttctctc
     5701 gttttttctc ccaaagataa agaaatttgg atttgccgcg gccgcccaga caggggaaaa
     5761 tatctaaaat attcgtttta aataaatttg ttggggctgt atcgaaaaat tcgaactgta
     5821 tttaagaaat tataacatcg ccccctcgtc ccgtcggcgg tcgggacgaa aaacgcgggg
     5881 ctcgattgaa aattattttt tgcgcgcgcg atttattatt aaatttcaaa attactcagt
     5941 ttcaaaataa atagctcttg gtatttttct attcaaatag tcttctaatt tgtttttact
     6001 aggtaaaagt agtgtagatc tctcaaagtc aaaatcaaat atttctagag tcatgattcc
     6061 agtattttga ttctttataa attctcttaa agatctagtt gaatatctat cttcagttat
     6121 agcgagtaaa atatgtattt ggtttaattt actttctaaa atctgtctgt ttttatcttc
     6181 aaactcacgg gttaatctaa tggaattatt aaaggaatta agcgggctca aaaatgttcc
     6241 actaaaaggt ctccagttta attgaataat taccactatt tcttcggcgt taccataacc
     6301 atcactcgga tgaaaattta tttttaaatg ttgtggtttt tcttttattt tcatggcaat
     6361 atctaaccaa tagttatgtg gtaaattttt aggaggaaca ccgcttactt ttattacagc
     6421 caagtgaaca tcattatgtc tagaacgtgc tttttcagta aagtaatcat caatatatag
     6481 gcataaatca gaaatcctct cttctctttc attttcacta atatctttta tgtgaaaatg
     6541 aatatcagta ttaaattcat ttactaaaat atccgtgtta aatggcttat tatcaacctc
     6601 aaaatccact acatttagat atttgttcag ccaaacactt actaaaaact gagtgaaaaa
     6661 tccaaaatta gtaaaaatac cttttttaaa taatttttta ctttttggat ttttttctaa
     6721 tacagaaatt aaacgaccta ttaggtacag gtctgaaata aacgaatact gttttgatga
     6781 ttcaaaatat ttgatctggt taaagagatt agagatatct attatctgtg gttttgtaaa
     6841 aaatgatttt tgagatacgg cttctatttt ttttaaacta cttattaatt tctttgtact
     6901 tatatcaggg ttacatactt tttttccttt aatttctgaa tatacttcac accaagattt
     6961 ataatctata tttacattat ctttcattat taccattgtt taattaaatc attaccaaca
     7021 acctttcaat acataatgtt taaactttat caattgttta tttaagggtt ttctttcaag
     7081 aaagtttaaa tttaaataat ttaaaagaag tggatttata aactattaag ttttgtcgct
     7141 aaactttctt gctcattata taatgcctcg tcttgatcta gtccgcttga ttcagaagag
     7201 attaaccact gattactcct cattctatta acagcatttc caatattaga tattctaata
     7261 cttagaattt cattcatcct gtcgcaaatg gatttattat cttcgtataa ttttccccat
     7321 tctttacatt tttgccatga tggttggatt tcttttaatc tagataaaac tttctcccaa
     7381 ctttcaatca ttgatttatc ataattagga ggaacataac caccaaattc ttgaactatt
     7441 acttggattt cacacccgtc aattttacca atgtacgagg ctataccagt atcttgaaat
     7501 tgtttatcta ataaattttt cttagtttca taatctaacc aataattaga taattcttca
     7561 gaatcataat aaccagtagc aattatttct ccagtaagtg tattactata accagcgtct
     7621 ttaattgctt ttgtataagg atattttcct ttttctcctt ctctaattag ttcgatttcg
     7681 ttgttatcaa taatagattt tgctctagat aaagcagctt tttctagttt ttcattcctt
     7741 ttaagaggtt ttaatttatt attttcccta atcttattta acctctgata gtaatcatct
     7801 aagttaaaag atggtaaacc atgatctact ccatcatttg aagcatctct taatctgtct
     7861 ttaaattgat cttgtttttt tatcggtaaa atactttcta tttcttttat tgaaactaaa
     7921 ccaacatctc ttgaatcttt acttcttttt cgattatcac ccaaagcaaa gtaataacca
     7981 tcaggtattt taatagtttt acaatctgct aaaaaagagc caccaaatgt tgatcttggt
     8041 tttgccgtat atggctcctt tactaactca ttatttacat ataaaaaccc atcacgaata
     8101 ctgacttcgt ctccaccaac agcaacaatc ctttttatat aactaacaat ttgtccatct
     8161 gtatttgatg ttctacctga ttcaaaagta acaatgtcgc ctttttgtgg tttaaaaaat
     8221 aatttattta aaattgtaaa tgaactcaaa ttaacatttt ccttatcaaa gatggttgga
     8281 ttcattgaat ttccctccac aggtgtgctt gatctaatta tccctaaaac taaaaggaca
     8341 aagaccatac tcaatcccca tacaggcaaa aaaacaaaga ttcctaagat agcactccaa
     8401 aaatttcgaa tgattaaaat aggtaaaaaa aatagtgaaa aaacaaatct aaaaaacttt
     8461 tttaatggtt tttccctaaa atgtttcccg taagaaagat ctaaagctaa acttatagcg
     8521 gttattagaa atgaaataat tccaataatt ataattacaa ttaaaaaata tgatggaatc
     8581 ttagaatttt cgtatgaaaa ctgaggaaat ttattagcca atagaactaa taaaaaaaat
     8641 aaagcaatag tgataaccca aattgttcca gaaatttttg ataatttaat aaattttttg
     8701 tggttttttg aaacaatagg attcttttca caagacaatt cgtgtttgtc tgattctttt
     8761 ttagttttaa attcctctcc acaataatca caagtccaaa catagtcaga atccttttta
     8821 gtctgtacta ccgttttaat tttatctctc ataactaaga ttattatatt ataaaatttt
     8881 ttgcgtttca aaatgacgga ttcttggcgc tggccaatag tgcgggagtg gcctgcgcca
     8941 catcacattt ctgtgattta tcaaaactgt tgtcagatat ggattcgaac cacaattatc
     9001 aggcccaaaa cctgaagtcc tgccgttaga cgatctgaca atgtatattc atcaactatg
     9061 actggttcta tcactcaaaa taacataaat caaatcattt ttcacaacca aagccatatt
     9121 caattaattc tctctaaatg taaaaacata cactaattaa aaacaagact aatttttaat
     9181 agctacaaga aaaccctttg tcaatttgac aatgaaaaat tttagtactt ttaagggatg
     9241 tctaagaaat aatccaaaat ctctaatatt atttaatgaa cattttttaa aaaatggttt
     9301 tttcccttta cttaaagcat ctataattat tgtttttgac aaataaacca tatcttttgg
     9361 taaacgctct acgctaaacc accttccttt acaaccatcg aattcaggct taaattttcc
     9421 atgaactttt cttccctcgt atacaaatat tataaaatta ttatttctat attttcctat
     9481 atatttaatt aactttacat caaaacccgt ttcctctatc gactctctta ttgctccatc
     9541 atcaggtttt tcacaatcat tcaaaccgcc accagttaat ccccacacag gaaaatcaga
     9601 acgtaaaacc aagaatatct tcgtctttga tttattaaaa agaagtaaaa atgatcctga
     9661 tttcatcttc cagtcaattt tgtagtccgt aaaagtgtct ctctatcaac aaaagttgaa
     9721 ccacaaccaa taatattttt tgcattttcg ccacagttaa acaataaatc aacaacactc
     9781 aaaccattca aaaatatttc cttttgtgga tactgcctat attccacaaa accaactttt
     9841 tgatacaaca catcaatttt attatttata aattcttttt catcaagcca caaagggcca
     9901 ttttgtcccg caatgtgaag atttgcacca aaaaatttac aagtcattaa gttgcaataa
     9961 gttgccttat tagactgatc tttatttctc tttttcgtaa aaatttcttt cggaaatgat
    10021 ttttctttcg taatatccaa gattttatct ttcaatctca atctatctgc taaaaaaaca
    10081 attaatctaa aattgtattc tccaagcgtt atacatgaat catcttccat ttgttccaac
    10141 aatttttttg tatactcaaa acctggtcct cttctatatg ttctataaag agtgtctata
    10201 tgatcttttt gtgatttcca aaaactttca cctagtacaa ttttattaaa cagtttttta
    10261 ttaacctttt ttccaatttt ttctatcaaa tataaaccat ctttttcgtt attttgcttt
    10321 aattgaataa tttttactct tttatgatga gtgccttttc gatttaacgg ataatagcca
    10381 tttataacaa aaacatcaca tgctttcatc ctatcaaaaa atcctatgta tggtaaataa
    10441 tatggttgat taataccaac aataatattt ttcatctata attttgcgaa aaaattaact
    10501 aaatcacccg tttgtaaatt aacacccaaa tcataataaa aatatctatc aaaagctggg
    10561 tcaccagaat aaattttatc cccatatatt tgaatatctg attttttctc caatacaaaa
    10621 ttattttttc ttttcaattt agttccacca ctttcaataa acctaagtgc ttcctcttga
    10681 cctaatagta attcttcgtc catctgttca ctatttggaa caattcgacc ttttactaat
    10741 ttagtaatta aacgcaacac tcttgggttt tgagtgaaac cttctataaa ccgtggtttg
    10801 ttctcgtacg acaagttttc tatatagtca cgcgttgacc gaattatata attatttccc
    10861 tgatattccg gcaatatatg tccagaacta aaatgaagca cagtattccc cttaaaccga
    10921 aaatatttat ttccatcatt taataaattt attgagtcaa atcctatcat tttattatct
    10981 ttttcaaaaa taaaaagaac tctacctcga tcaagtgccc caaatataaa tttttcgatt
    11041 ccttgcttcc ccgagcttga tacaaatttc tcacaggtag tactttctaa aacttctgat
    11101 gcttctatta tcaacttatt tctagactct tttgataaaa ttcgtaaagg atttcttatt
    11161 tctcgacaaa caatatctga atattggatt attttttcat ttgtacacgt tagactgtcc
    11221 ttttcgttga tattcataaa tttaatacat aaatggattg attatagcca atactgccat
    11281 tttttactac tttctagtaa aaaacattat cccgaaacat aagatattag attctaaata
    11341 ttattcgttg agtcgaacaa aacttacacc ttgtttcatt ctgtgattat gaaaaactgt
    11401 tgccccgcat ggattcgaac canngacgac ggggcattgt ccgtctttct agtgccgacg
    11461 agaggacttg aacctccaca ccttgcggca tacgcacctc aagcgtacgt gtataccaat
    11521 ttcaccacgt cggcaaattt tcaaagaact caatattata tcgcaaaagt ggcctcgcca
    11581 atttattgct tttctgggtg gcatgccaac ccgtagcttt agcgaagggt ggctgttgag
    11641 gggctcgaac ccccgacatc atcgatgtaa acgatgcgct ctaccaactg agctaaacag
    11701 ccaaattttt aaaatagggg agcgggtaga gggaatcgga ccctcgccga gagattggaa
    11761 atctttcgtt ctaccactaa actatacccg cgtttgtaca ttattttatc aaaaatgatt
    11821 ttaaatagta cttttagggt aaaatatagc agtctgccgt cgtgttgaaa ctggtagaca
    11881 agcacgtctc agaagcgtgt ggcgcaagcc atggaggttc gactcctctc gacggcacca
    11941 tacatttaat cttttttttg ttaaaataag catacttctt tgccgaagta gccaagatgg
    12001 tcacggcact ggtctgaaaa acctgggatg ttggttcgag tccaaccttc ggcacatttc
    12061 ttatgaaatt taattagcgg gtatagtata gtggttacta tgcgtccttg ccaaggacga
    12121 gtcacgggtt cgagtcccgt tacccgctca aaaatgccgc tgtggtggaa ctggtatacn
    12181 nnnnnnnnnn nnnnnnnnnn cccgccgtag ctttagcgta ggcggggatt ggcgcaattg
    12241 gctagcgtgc atggtttggg accatgaggt tgcaggttcg aatcctgtat ccccgaccca
    12301 ccaaaagcta cgcattttgg cgggtcagcc ataacgtgca aagcacttta ggctgactta
    12361 tactaaatga atgttttatt acacttacgt gttgttatgt tcaaatagat tacaaatcaa
    12421 gaataaattt tatattggtt acaccaaaga tattaaaaaa agatatcaaa gacacttaaa
    12481 taaagaagta aataccacta aaaaatttga ccaattaaaa ttaatttatt ttgaaagttg
    12541 tttaaataaa accgacgcta gaaaaagaga actacaatta aaaaccggat ttggtagagg
    12601 ttacattaat aaaagaatta gtacatattt aaaaactatt aaaaaatgac attgaatcgt
    12661 cctcaattct ttaaaaccct taccggtaaa aaaattccct taccaatatt tttccccgat
    12721 gctactcggg ctgtcttaaa aactcttgat tccgaagata taaaaaatac ccatacttta
    12781 ggtgttttag tcaatacctt tcacctatat caagatcccg gtcatgaagt aataaaaaat
    12841 ttcaaaggtg ttggcaactt tatgaactgg agtgggggaa tcatttctga ctccggtggt
    12901 tttcaggtta tgtcccttgc caaaaccatg gggggaaatc aggctgtcac tgatatcggc
    12961 gtcaccttta aaatggatgg taaaaaaatt ctcttcactc cggaaatgtc gattaaactt
    13021 caacttgaat taaaaagtga tatggttgtc gttctcgacg actttaccga ccctaaagac
    13081 aactacgaaa aggcgaaaat cagcgtcgag cggaccctaa aatgggccag ggaatgtcga
    13141 caaatttttg ataaattaat cgctcaaaaa aaacttacta aagagaatac tccctatcta
    13201 ttgggagtag tccaaggcgg aaaatattca gatttgcgaa aatattgtac gcaggagcta
    13261 gtcaaaatcg gttttgatgg ccttggctac ggtggctggc ctatcaataa agatggcacc
    13321 tttgattacg aatcagctca aataattgcc gacagtaccc ctaaaaatta ttttctctac
    13381 gcccttggta tcggtaaacc ccacgaaatt acggcgctcg ttaaaacagg ttggaatatt
    13441 ttcgactgtg ttctccccac ccgtgacgcc cgccacaaac gtctatatgt cagtaaaaat
    13501 caagattata gtgattacga atactacacc cctgataaaa atatttatta tcgggaccag
    13561 cgtcctgtct ctgaattttg tgattgtctt ctttgtcaaa actattcccg gtcctattta
    13621 gctcatcttt ttcgtatcaa agaaataact gccctaagac tggccacaat tcacaatctc
    13681 cgtttttatt ccaaattaat ggaaactctt agacctgtct actctgagtt ataatcattc
    13741 aatgccaaaa acaattttgg tcactggtgg cgccggtcac atcggctctc atattatcga
    13801 aattctggtt aaagactcaa acaataaggt tatttccctt gacaattatt tcactggaaa
    13861 attagaaaac catattgatg gagctcagta tcgcactggg cacaccaaag acattgataa
    13921 attaatacct gaaactccag atattgttta tcatctgggt gaatatgccc gagttgctcc
    13981 ttcttttgat gatgtcgaaa aagtctatga tctcaatatt cttggtactt ttgccgtcgt
    14041 taatttttgc caaaaaagaa aggttggcaa gctggtttat gcggcctcta gcactaaatt
    14101 tgcaatagag ggaaatggtc gacatcaaaa tccttattct ttttccaaag ctacaaatgt
    14161 cgatttaatt aatgattatg gccgttggta caatttaccc tacgctatct gctatttcta
    14221 taatgccttt ggaccaagag aaaatggaat aggcaaatat gccactctaa ttgccaaatt
    14281 tgaacagatc tatcttaaag gagaagctct gactgtagtt aaacccggca ctcaaaaaag
    14341 aaacttcacc tatgtcggag acctagctca ggggattatt ttagttggcg aaaaaggcct
    14401 aggtgataac tatgccctat gtaattcaaa aaaatattct gtcattgaaa tagctaaagc
    14461 ctttggttct cccattaaat atattgatgg atatccaggt cggaatgaat ccgacaccgc
    14521 ccccacaaaa gcccgggaag
//
LOCUS       DHGB01000025            4564 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_172469, whole genome shotgun sequence.
ACCESSION   DHGB01000025 DHGB01000000
VERSION     DHGB01000025.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_172469"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1385 a         1087 c          644 g         1448 t
ORIGIN      
        1 ccctgacttt taatcgatga ggcccatctc ctagattaaa aacgccctca taaggcctct
       61 cggtccatct cttaacttca ctatcatcaa tccacagttt gacttcagta atttttttaa
      121 gtgctgaaat actaattttt atttccacat tattgccctg agttgaacgg tctcccggac
      181 tatcaaaatt tactcccacc gccccatcac tgcgacaaaa agattcgggt ggaaaatatt
      241 tatccttatc actttgttga tttatccagc tatcaatccc ttcttgccag cggtttcgcc
      301 catctttact gaccggatct tcttctttaa aattaaaata ttcttttaaa tcatagttac
      361 cgttagccac atcctcagga gtcgccaacc ctaccttgtc ccggcacact tttaatttca
      421 gatgaattgg gtcaggaaca ttcgtttgcg ttccttcaat aaaatactct gttttactag
      481 gaaactcatc atggactcca tagcccgata ctttatcaac tgaaatattg actatgccag
      541 cgggaatttt aaaatcttct ttttcccttt ttggtaaggc atagagcata atttttcgcc
      601 aaattggggc cgctcctgag actccagaag cgattctccc catcggagtg ctgtcattat
      661 tccccaccca aacagttacc agcaaatttg gagtccaacc ggtagtccag ttatctttct
      721 tttcattggt agttccagtt ttgaccgcta ctttataatt gggaataatc agactattta
      781 ccgcaccaaa tgtcaacgcc cgggcgttat tatccgctaa aatatcggaa ataataaaag
      841 cctcctcagg actcaccact tgttttccct ttattggatt aaactcttcc agaaccctcc
      901 catctcggtc ttcaaccttt aatattccca ctgcttctac cttattccct ccattggcaa
      961 aagcactata agctgccgct aaatctatca ttctaacttc agccccaccc agagttaccg
     1021 caaagccaaa ttttttcata ttttctgccg ttggttccaa atcattaatt cccatctcaa
     1081 aggcttgctg gagcatatta ctaactccaa cccgggctag tgttttaact gcgatagtat
     1141 ttatagaatt tccaagtccc tctctaagac tcatcggacc attaaactta ccactataat
     1201 tttgagggca ataatctctt tgtccagcaa ccggaaaaca aaccggtgtg tccataatta
     1261 aactcgccgc tgaataaccc tttcgcagcg ccattagata ggtaattggt tttatcgctg
     1321 aacctggctg acggagtcct tgagttacca cattaaactg accttcagtt ttatcactat
     1381 tatagtccct cgaccctacc atcgcgagta cctgaccatc tcgaggatca agaattaccg
     1441 ccgccccatt actaatttcc agtttttctg atttatcgat ttcttcggcg actatttttt
     1501 gaacttcatt ttgcaaactt aaatccaaag ttgttgtcac ttttaaaccc ccaccctcag
     1561 cgatgtcttc accatatttt ttagacaaaa tatcttttat ccaaaaaacg aaatggggtg
     1621 cctgaagttt ggttgaattt tggaaaaact gataattttt tacttcttca gttgtcttaa
     1681 tttgagtttc tttatcgata tagccatcct ccagcatcct gtttaaaaca tgctctgttc
     1741 gagctatgta agcctggggc gatgaggaaa agggagagta gacacttggt ctttggggca
     1801 aacccgccaa aatcactgac tcagccaaag tcagatcctt aaccgcttta ccaaaatatt
     1861 gctctgccgc gctacctacc ccccaagccg ctcctccata aggtgattcg tttaaataca
     1921 tcaatagaat ttcgtcttta ctatatctcg cctctatttg aaccgccaaa ataaattcct
     1981 taatttttct agtaatacta acttcagaag ttaatagaac gttttttact aattgttggg
     2041 ttaaagttga accaccggtt aattttttaa aataaaataa atttttaata attctaaaag
     2101 gcgccaaagg atcaaaaccg ctatgtttgt aaaaatcctt atcctcaacc gcaatcgtcg
     2161 cttttttcag agcctcagga atatccttaa attcgaccgg ttgccgtttt tggtcttgat
     2221 aaagatcata taaaatttcc ccattcctat cgtaaattcg actggcaaaa ccctctcttc
     2281 gaaccacttt actgggactc ggcaaatctt ttgaatacca ggcaaaaaga cccattgttg
     2341 tcagtaaacc taccccaaaa agaatgagta tcacccaaaa aaatcgatac caaaacttct
     2401 tactttgaaa aacctttttt aaacgacacc accagagttt cattttatta aagtactcaa
     2461 aaagggaatc atccctgata acaaaatggc caaggtcgcc actactaaaa ttgctgtttt
     2521 caaatgtttt ttctttattt tttttccaaa aatcttcatg aataatgata acataagcca
     2581 tgagcttctc actccctggc attggttcca aaacaagcgc taaactcgaa aaactaaaga
     2641 ttaataatga tctcgagctt ctttatcatt ttccccatcg ttacctagat ttttcaaaat
     2701 caataaaaat atcggaggca cctcttaacc aaaacgtcac cattagtggt caaattcaaa
     2761 gtattaataa tatttttttt cgaagtggaa aaagtctgca aaaaatcgtt ctcactgatt
     2821 ccactggcac cattgattta atttggttca atcagccata tttaataaag aattttcacg
     2881 aaaaagaagt ttgggctttc gccgggaaca cttctcttta tcgcggccat aaaactattt
     2941 tttctcccga gtatgggcaa tataatacag gcaaaattat cccgatctac cctgaaacca
     3001 aaggtttaac tagccgctgg tttcggaaaa ttatcagcct ccactttgac tcattagtta
     3061 aaaatataac ggatccttta ccttcaaatt tactttctgc ttttcatctt atgcccctta
     3121 acaaggcctt aaaagaaatt catttccctc aaaatcaaaa tattttagac aaagctcgat
     3181 atcgcctggc ttttgatgaa ataacttctc tcttagcttt aagtcaaatt caaaaaaaat
     3241 cctgggctaa tttttctcca aaattcattc taaaacacta ctccactact aatctaataa
     3301 actctttccc ttttaaactc actgattccc aaaaaaccgc ttggcaagaa attaaggctg
     3361 atttgctatc taaaaaccaa gttatgaatc gtttactttg tggtgatgtt ggctccggta
     3421 aaacagtcct ggctcttctc tcagcttatt taacttctca aaaccagaaa caaagcctca
     3481 ttctcgtccc caccgaaatc ttagcccgac agcacttcgc cacctttaaa cagtatctgc
     3541 ctgactaccc gataatttta ttaaccgcca aaaattctct tcctgaaatt atgcctgaaa
     3601 atgccattat tattgccacc cacgctgcca tatttcaaaa agaaaaattt gtcgacaatt
     3661 tagctttatt aattattgat gaacagcata aatttggggt caatcagcgc tcatttctta
     3721 actcaactca gccacctcat accctcacca tgactgctac ccccattccc cgcagcatca
     3781 gtttaactct ctttggtaac ttagatttat caactctaaa aaatttacct caaaaacgct
     3841 ccccggtcaa aacttttctg gttcctcaaa ataaaatttc tgattgttat cactggcttt
     3901 tagccgacat ccaaaaaaat aagacccagg cctttatcgt ctgtcccttt attgagcagt
     3961 ccgaaaacgc cgccaccatt aaatcagcca aaaaagaatt tgaaatctta aaaaatattt
     4021 ttccaactct aaaattagag ctaattcatg gtcaaactaa aatagaaatt agaaaccaga
     4081 ttttggacaa atttaaaaaa aataaaataa atatattagt taccactccg attatcgaag
     4141 taggtattga ttttcccaat gctaccacta ttattattca atcagctgat cattttggcc
     4201 tctctcagct tcatcagctc cgtggccggg ttgggcgggg gacacttcaa agctactgtt
     4261 attttttcac tgaaaatacc aatgaaactg ctcttcgtcg tttaaaatat cttgaaaaaa
     4321 ccaccgatgg ttttaaaatt gccgagtttg atttaaaaac cagaggtccc ggagaaactt
     4381 tctctaccct ccagcacggc tttccttcac ttaaaatcgc ttctttttct gatttaaaat
     4441 taattgaaac atgccaatta tttctaaaac agcttcaaga taattatccg gaattcgact
     4501 taaagaaatt aattctcaat caatcttctc tttccttaac cggaaaacat ttaaattgaa
     4561 aggg
//
LOCUS       DHGB01000026            6011 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_56537, whole genome shotgun sequence.
ACCESSION   DHGB01000026 DHGB01000000
VERSION     DHGB01000026.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_56537"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    4256..4305
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2085 a          750 c         1427 g         1697 t
ORIGIN      
        1 tgagccctcc gcgagaatcg aacttgcgtc gatggtttac aaaagatggg agaaaaggga
       61 ttggaagact gagaatatat aattgataat tatctattaa agtgatataa actacatcat
      121 gttggggaga ttaaaagaaa cattaaataa aggtgatgaa tttgatcaag ttcaaggtaa
      181 gattattgaa acaatattag caatccgtcg ggaacaggga aaaaatgata taccttttcc
      241 agcttcagaa caagctttga gtatagaaga ggctgtagaa aaatatatag aaaatcctaa
      301 taattctgaa tgtttaactc aattgatgcg tagtttttgg acaaaagctg atgaaaaaag
      361 tggcaaaacc acgatggtac ctgaatttcc cttaaaatct aaagaaatta aaaagagagc
      421 caaacaaaat caaatggcga tttttaatgg agatgaatgg gtatggattg agaaattggc
      481 taatgcgccc aatttgggta ctacagaatc ccaatttaag aaaaaacttg gaaaaggggg
      541 tagacagatt ataactttaa aaagctataa agttggagga gaaattcata agttgttgta
      601 tggtatatat tttgatcaag aaggagctcg gtcaagagta ttaaatgctt atggtcaaat
      661 tgttgacgcc tatttcattg acggtggcgg catgtgtgtc cgttgtcggt ctttgccttc
      721 tgagcgttgc cgtgattatt tgggtgggcg ttttgaggaa gtgataaaaa agccaattaa
      781 atgagccctc cgcgagaatc gaacttgcgt cgatggttta caaaaccact gctctaccat
      841 tgagctagga gggcgtagat agagtattat agaataggat atggtgaaaa agaaaatttt
      901 aattctgact gatgatttgc cctggggaca ccgttcgatt gctaaagcca tatacggatc
      961 acttaaagaa aaggaaaaag aaggtgcggt tgaggtgaaa tatgccgaga ttaaggcgga
     1021 agttggaatt tctaatgatc tttatacttt tatgtatcgt tttttcccga ggtcaaacaa
     1081 gatgatgacg aagttgtcag agagggggag attaagaaaa gtggttcaga gattgtcgga
     1141 gttaaattta cccagaatta aaaaatttgt ttctgtgtat aaacctgatt taattatttc
     1201 ctgctactgg tatcacagtc attcattgca actctggcga aaaaatgaaa aggtaaattt
     1261 taaactgtgg tcagtggttg ccgatccctg gacgattact cccatgacgg tggtgcccgg
     1321 agtggatttg accttggtct atgacgaggt ggcggagaga gaggtaacaa aatatgggct
     1381 gaaaggaaaa atattacaaa gtggctggtg ggtgagaaaa gagatgtatc aaaaggtcga
     1441 cagaaagtta acaagaaaaa aattgggaat tatggacggc cggccggttg tttttatcgg
     1501 tggggggagt ctggggacca attcattgac tcgactactc ccagttttaa tgacgattga
     1561 gacaaaatgc gcttttattt ttaataccgg aactgacaag atgctccact ccaccattga
     1621 tcagtatgta gaactactga aaaaattaaa gctaggaaag caaattattg tcaagaactt
     1681 agggtggata gagaatatgg cggaggtgct taaagcttgt gatattgttt ttggaaaagc
     1741 gggaccaaat tttttgtttg acgtggtcgc ctgtcaaaaa ccgatagtgg cgataactca
     1801 tattgggggg caggaggatg gaaatattga aattataaaa aagaaaaaat taggctgggt
     1861 aaaagaaaaa ccaggagaat cggcatcttt tttgcttgaa tacttagaaa aaccaaaata
     1921 ttatgaaaaa atattctcta aaaacattga gaaggaagca aaaaataatc agatgacaat
     1981 ggagaggatt tggaagagga taaaagagga gataatagag aaaatctaaa atctaaaatc
     2041 taaaaaaaac gaaaaagcct gccaaaagtg gttacttcga tagtggcttt tccacgcata
     2101 ataaaacacg tggggaaaac tggttagtaa aggcaaagcc gataccaaca cgcgtcctcc
     2161 ttttttttag gttagctaga aaaaacacct tctcaaagat cgctttttgg caggttgtat
     2221 aattgtacca tgatggccat ctcaaaaaaa gttgaatata gcatcgtatt tatttcatat
     2281 ttatcgcata ataagggtaa aaatattact attagtcagg cggccaagat tttaaaactg
     2341 ccttacagtt ttttggggca ggtagcagta aatttaaaat ctggaggaat tattgaggct
     2401 tctgagggga aaagtggagg gtatagattg attaaaagct ggaaaaagaa gactttgttt
     2461 gatttatttg aagctttggg cgagaataag gcgatagtta cttgtttggg tgataagggt
     2521 tgtttaagag tcaaaaactg taaaatgaaa aaaatttggg agaagataga gaagagttgg
     2581 tttttggagc taaaaaagat aaaattggga gaaatttaaa ataaaaaagc ctaaagcata
     2641 aaatctaaaa tctaaaaata aatatatatg ataaagaaaa aaataaaaca aaaaataaca
     2701 aaaaatacgc taatattgga gatagcggag aggtatccga agttggttga aattttagtc
     2761 gaaaaatatg gtcttcattg tcttggttgt tctttagcgg cggttgaaac tttggaggag
     2821 ggggccatgg gacatggaat gagtaaaaaa gaaatggaaa agatgatgca ggaattaaat
     2881 aaagaggcta ataaagtaga tttgaaagga agtaaaaaat cgggtaaata attttgccaa
     2941 gaaggccgga aattaaaaga agggcgatga gaagatagcc ataatgctca aaagaagctt
     3001 cgattttggc agcctcgtca accgggagaa gatttaaaag aattttggat ccgtccaagg
     3061 ggggaatggg aaggagatta aaaataccta aaaataggct tatatttcca acataggtgg
     3121 caaaactaga atgaaacaga gagagaagga aagccagaat aaaattactg agggggccgg
     3181 caaaagcaac aatggtttcg tcacgacgag gatttttgaa attataggga tcgataggag
     3241 taggctttgc ccagccaatg acgggaaagc cactgagggc gccaaaaagg ggaagcaaaa
     3301 tggtacctat agggtcgaca tgagcgattg gatttaaagt gagtcggcca taagcccggg
     3361 gggtggggtc gcctagctta tcggcggcca gggcatgaca aaattcatgc attgaaataa
     3421 caataattaa tgaaaaaact gataaaaggg caatgatgat ttccatattt tatttggaag
     3481 ttgcttttgg tatagtatac cctatgagaa aatgtagttt gtgtggaaag ggaactgcta
     3541 ttggtcgaaa tcgttcccac gctcaaaatc gaacttcaag aacctttaaa gtcaacgtgc
     3601 aaaaagtttc tctaaaagta ggtgagggag aaataagtgg ggtttattgt ggtaagtgtt
     3661 taaaaaagat aaagggagaa attaaaaaaa cggcacttaa ataagtaccg tttctttaag
     3721 agaaaaagga agtttatttn nttcttcact tttttcttga cgactttttt cttggctttt
     3781 ttgacgggca ttttttattc ctcctttata aaattaaaga ggatttttag tattttgtca
     3841 agataaaatt tacctattat atcgataaat taaaataatt taagaataaa atatcataaa
     3901 tattttaaaa aatttattta agttaacaat aaaaaatttt gtgttttacc ggaataattt
     3961 aatatcagtt ttttctaatt agagaataat attttttaat ctcattccac tcggttttgt
     4021 tttttccctc aatttggact atttctggga tgagatctga tttattttga ttaataaaag
     4081 cggaaaatat tttcatttta aaaatgttgt gatttagatt ttccacaaag gtccaggcaa
     4141 ttggccaggg atttaaggcg cgaataaaag cttcgatttt atttagattc cagctccaat
     4201 ctatttttgc cgtatcttta gttaattttg gaaaataact tttttgagaa tggtcnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtggt aatgataggt
     4321 ccatggtcag gcagagggtc gatttggaaa aaagtaacgg cacttttggt ttcaccatta
     4381 ataatttgcc attggccggg ggtggcgcca cgatattttg gcagaggact cgggtgaatg
     4441 ttgattattt tgtaggaaaa gctattgata atatcttcat caaaaaatgg tggaccaaag
     4501 gcaacacaga ggccgagatc cgctttaaaa tctttaatct tcaatttaca atcttcagaa
     4561 aatgtctcaa tttgaagaag ggaaagatgg tggctgagac agtatttggc tacgggattg
     4621 ggagtaattt tttggctccg gccaaaggct ttgtcgattt tggaaacgac aatctggagc
     4681 tcaaagtcgg gtagctcgag taatttttct aaaattggca aagaatagat tgaggcgcca
     4741 aagaagataa ttttggtcac agtatagatt ataactaaat tttgataaaa ttaggatatg
     4801 agagagatag tcaaatttcc aaataaagtt ttaagaaaaa aaagtgaaaa aattgaaagg
     4861 gttgatgaga cgctattaaa agaaatggaa gaactcagag aaatactggt ggcgtcggaa
     4921 aacggagcag gcctcgccgc acctcaaatt ggaatttcaa aaagattttt ggggattaaa
     4981 aacaaagaag gggtaaaaat ttttataaat ccgagaatga cggcgagctt tggtgaaaaa
     5041 atttatccta aagtttttga tgaggaaaag aaggaaagtg attttttgga gggatgttta
     5101 tcttttccgg atcttttcgg aactgtgaaa aggtatttaa agattgaggt ggtctgggaa
     5161 gaaatagtgg gaaacccgcc aaaagctaaa gcatttaggc gggcaagcaa gattctagag
     5221 ggatttgagg cgattgtttg gcagcatgaa agcgatcatc tggacggggt attgtttgtg
     5281 gatagagtaa aagaggaggg gggtaagttt tatttgtgga aagaagagaa aaaagtgccg
     5341 atggatgtta atgagatttt ggctaaagag aaataatttt ctctaaatat tcttttgctt
     5401 cggaatttgt gggggcatac tgtagggtaa tatcgaagta ttttttagct tcgggataat
     5461 ttttttgatt aaaatataat tcgccgaggg cgaatgaggc atggtcataa tttgctttaa
     5521 gtgtaagtgc ttgcttataa ttatcgatgg ctaaatcggt tttggagacg gcttcataaa
     5581 atttaccgag tagataaaag cttttggcat cggtgggagc taggcgaaca gtatttttta
     5641 gagcttcaat ggctagggaa aaatagtctg gattaatctg ggataattcg gaaaaaacct
     5701 gagctctttc tttccataga ttagtggaag caggggaaag ggagagagct aaattagaat
     5761 gggtgatggc gcgaggagca tcttttaatt tagcagcgtc taacgcggct tgactatgat
     5821 aaagagcttc tttgggtcga agggaaagag ccgtgttgat gtatttttgg cttgaatcat
     5881 agaggccgcg attgtaagca atttgggact gattataggc aatatcggcg agaaaaaagg
     5941 ataagatttt agaaattaga aataagaaaa gagggataaa aatgaaataa aatatttttg
     6001 attttggttt a
//
LOCUS       DHGB01000027            4888 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_124336, whole genome shotgun sequence.
ACCESSION   DHGB01000027 DHGB01000000
VERSION     DHGB01000027.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_124336"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1491 a          971 c          922 g         1502 t
ORIGIN      
        1 agagtagtaa tcgttaaagc ctgggtcaaa ccacgttgaa aaagagcgga accatgagtc
       61 attggtagaa ctccagcttc tatctcgaga ggacgaattt catcaagggc tcttttgtcg
      121 aagcgtgtac ctttaagagt cgcctcgcga agatattggc ggactaaaat atcaacagct
      181 tcaattattt gattagcttc aacctgctcg gcttttattt ttgcttcgaa agcctcttta
      241 accttttctt ttattttttc ttctgagcta atatgagaac cgtcaatacc gccctgcata
      301 aactcatcaa gttctttttt aacaagctct tcaacctcag taattaattc ttttgaggga
      361 gagagaggct cgtactgaat ctttttttta ccgtattttt tggtaaattc gactaactgc
      421 tcgtttattt cgtcgccagt tttgaatccg agttctatag cctttaaaac agtctcgttg
      481 tcaacgatat tagcatcggc ttcaatcatg ttgataccct cgcagtcttt gcaaactaag
      541 aggtctaatg aagatttttg aagatcggta atagtcggaa acagttttaa ttctttgttt
      601 tctaggccga ctctaacaat agaaaccggg ccattaaaag gaatatcgga aagcatcaga
      661 gcggcggaaa cggcggtaaa agcgggaata acgacatcat tgtctttatc gttgctcaaa
      721 accgtggcaa ttacctgaac ctcatatttg aaatcgacag aaaataaggg acgaattgat
      781 ctatcaataa tacgaccaaa gagaatggcg gtgtcagaag ggccaccttc ccgcttaatc
      841 cacttaccac ccttaattaa cccccctgca taaagcttgt cggcaaattc aaccgaaagg
      901 ggaaaataat ctttactttt atcaatagct cccaaaacaa cggtcgccaa aacgacagtt
      961 ttgcccattt gagctaaaac agctccgttg gcctggggag ctatttgacc agtagtcaga
     1021 gtaattttct tgtcaccaat gacgatgtcg gttgaatttt taatcatttt tattttttaa
     1081 gatttaagct cttaataatt ttttggtaac gatcttcgtt gtgaattgac agataacgaa
     1141 gcaaacgacg tcgcttggct agtttactta aaagacctct tttggctgga gaatcatgtt
     1201 tattttcacc taaatgtcct tggagttttt caatttcttt ggatagaatc gcaacttgga
     1261 tttcaggaga accagtatca cccttggcct gggaatattt agaaataatt tcgctttttt
     1321 cttcggtagt taatgccata gataaaaacg ctaataacgg taaaatataa tatttcagaa
     1381 tggctactga aaaaccgtat ttagtgtatg ggtattgtag cacaaaagcc ggaaattagg
     1441 cggtgggagt aggattgggt ttattggctc cagaaagcaa gttgccagat ttttcaatat
     1501 tttcaatggg catagcttca atgggagcag ctatattttc attcggctgg gtagcgcgca
     1561 gacaaagagc cccagcttca aaagtttcag gagaaacatt gacactgaaa gattgagtgg
     1621 ctactcgaag accgattaat aaatctttag ctgaatcaat atctaaattt aaaccaagca
     1681 taggtaataa gaaagtaaat atttctgaaa aaggagcgtc aggatcaaaa atagtagctt
     1741 tagcccaggt cttattaacc tgagaggggg taatcagaac ccaattacct tggttaacca
     1801 tcgaggcgga aactccaagg ggagcttcat caccaagcat aaaacaaaca tcagccgcgg
     1861 aaccattatt attgatgaca atttgattgg tttcgacttt agcagctcca gatttggttt
     1921 taacgactag atttaggtga ccgccatcat cgttataaga aacagcttca atttgatcaa
     1981 tgggatagtc caaagagaca actaaatcgg aattagaata attatcaact aaaagctcaa
     2041 gaccgcttaa tttttgataa ttttgatcgg gcttttgggg gcaaaacacc ttaacctgtt
     2101 tacctagttt ttttaaagan nagggcgctt ttggcactgg taacaagatt tttaatttca
     2161 gtaacattat aatttttcat atttgtcctg cttacaaata aatgactatt ttgaattctt
     2221 ataggctatt ataacatcgt ttattttaaa gtcaatatag ggacgaaaag tcataccaca
     2281 atcatgacca actttaattt tttcaacaat gtctttacct tggcgaatac cctcgatttg
     2341 ggtatttttg ataattttac cctcacgcat gagatgaaca gaatcacctt tagtcatttc
     2401 acctttggta atttttatcc cagcaatacg cactttttca atcttaaatt cagcgataat
     2461 agtcccctca ccgacaatgg tatcctcaat cgtgggatca agcattttca agacctgggc
     2521 ctgaatattt tcaataattt cgtagattat ctttgattcg aaaatgggaa ctttctcatt
     2581 atcggctaag tttttgatag ttttactgac gggaacatta aaggcaaaaa gctcggcgtg
     2641 actggttttg gcagcaatca cgtcattgtc attgaccgga ccaacaccag tgtaaattaa
     2701 attaacgtca tcactaagac tatttttgag agcttcgagg gtcccctcgg tatcagcttt
     2761 aataacgata tttaaatgag gaaggtcggg attaattgga aaatttgtaa ttggtagaga
     2821 cgcatcatga ggcgtctcta cggggttatt tgtaatcata gcgccaacat aggggacttt
     2881 ttcaaatccc aagatttcaa ctggggtact aggaccagct tctttaatac tttttcccga
     2941 ggaatcaaaa agagccttaa ctttaccaga aatactttcg gtaaaaattt gatcacctag
     3001 ctttaatatt ccctttttga caataacggc agccacagga ccacgggcgg tatcgagttt
     3061 tgattcgacc acgaccgctt ctagggggga ctcggattca tcttttaact ccataatttc
     3121 ggcatttaat agaattaact ctagcagctc atcaataccc gctccagttt tggcagagac
     3181 gggaacaatg gctacttgac caccatactc ttctggagtt aggtccattt caacgatttg
     3241 acccttgacc atttctggtt gacgagtctc taaatcatac ttagtcaaag caataattac
     3301 aggaagattg gcctttttaa tttgagcaat acactctttc gtttgaagca tcactccgtc
     3361 agtggctgaa ataactaaaa ccaccagatc ggttaaattg gcacctcggg tgcgcatggc
     3421 acaaaaagcg gcatgaccgg gagtatcaat aaaagtgatt aaaccggact taccactttt
     3481 taacttgact tctgtttgat aagaagaaat atgctgagta ataccgccag attctttctg
     3541 agcaacgtga gcattacgaa tgcgatcaag aagagtcgtt ttaccgtggt caatatgacc
     3601 catgacagcg acaataggtg gacgatattt gctattcata attattattt aaatctacaa
     3661 tcttcaatct acaatcttca aattacaatt aattaataaa cacaaaataa tttaatttcc
     3721 aagttaattg aaaattgata attgttaatt ttttttaacg gcactgactt tgatatcagc
     3781 atcagtaaga attccggcca ggcgaacatt ttcaccgcca gccccaatgg cgagagccaa
     3841 ttgatcttca ggaacttcga cctgggccaa attatttttt aacgaaataa ccttgacatt
     3901 tgaaacagga gaaagagcat taacaataaa ctgggactgg tttttggcga aagcaactac
     3961 atcaactttt tcatcggagg gaagctcact taaaattgat tgaattcggg aacctttttg
     4021 accaatacaa gaaccaacgg gatcaattcc gttttgggaa gaggaaacag caattttagt
     4081 tctttgacca gcttctcggg caattttttc aacaactaca gcccgattac caacttcggg
     4141 aacctcacgc ataaaaagtt gaacgataaa ctgaggatcc cgacgagaaa gaataacgtc
     4201 ttttttaccg gtggcttcgt cttcacgaat ttccttgatt aaaaataatt tttttgatcc
     4261 gatttctaga gattcatttt taatctgctc ttccttagga cagatgcctt cagtcttacc
     4321 aattcccact gtcattcgat aagggtcaat tcgtaaaata actcctaaaa ctaatgttcc
     4381 ggttttttga cggtattcat cgatgatggt gtcacgttca acttcgtgaa ttttttgatc
     4441 aataacctgc ttagccgtca tcgccgcaat ccgaccaaag ccgggggggg tcacattatt
     4501 ttttttatca ccctcaattt caaagatagc aaaggaacca gactcaggag ctaaatcaac
     4561 ttcaaaaata gcctcttcgg gtacaataat accgtgttct ttttgatccc ttttataggc
     4621 agaaattaaa ccctcctcga tagcatcgat gatttcctgg ggattaatat tgcgctcgcc
     4681 agcaatttga gctacagcgg cggcaaattc tgtttttggt agtcttttga tatccatatt
     4741 tatattttgg taaataaaaa tggggacacg acggccccca ttccaaatac cacaaattaa
     4801 ctttagtatt ataacactaa aatggcttaa aactgataat tacagcccca gctctttgag
     4861 aagatctttt tgtttggcgg atagacgc
//
LOCUS       DHGB01000028            3285 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_99408, whole genome shotgun sequence.
ACCESSION   DHGB01000028 DHGB01000000
VERSION     DHGB01000028.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_99408"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT          996 a          659 c          583 g         1047 t
ORIGIN      
        1 ctctccgatg ccaaaaacaa tcccgataaa aaccacgaca atatttttaa atatattaat
       61 tctgaaataa atcacattat taccgccaat caaaattttg aatcaggaaa agttgatgct
      121 caaaaattat ctttggaatt ttccgaaatt ttacctattt ctagtgctga tcgaattaag
      181 ctaatcaaag acattgataa gtcggataat ccatccgaat tattaaactc acttctcctc
      241 aaacagtggc agcaacgagt cgattatttt ggtaaagatc tagccaacaa tattttggct
      301 tatgcggtca tcaatacact tgatcctctt tgggttgacc atttgaccgc cctcgatgat
      361 ttacgtgacg gggtacgtct gcgcggttat gctcaaaaag accctctggt tgaataccgt
      421 aaagaaggtt ttgagatgtt tcaagcctta atgcgtcgct ttgaatcaaa tttgtcacga
      481 atgctttttc gtcttgagcc agtggcggct ccaatggcca ctcaggtaaa agcgaccgaa
      541 gctaggggta aaatgattga ccccaatcag ccgatagatg gcgccgttga cgattctgac
      601 gtcattgcga gcgaagcgaa gcaatctctt cccactcgcg aaccagtcgt aaatactggc
      661 aaaactttag gtcgtaacga tccttgctgg tgcggctctg gcaaaaaatg gaaaaaatgt
      721 cattaccctg agctgggata agttgtggga taataaagaa atggttttcg ataaattatt
      781 taattttcat cccaaagaag ttattaaatc tgtatcttct cgaccttatg gagaaacaaa
      841 tattggcatg cgtcgagaaa agaatgaaga tgctatttta atttttgatc gcaatgatat
      901 ttatgaccaa attaaattac atagtgttta tgcagtggct gacggagttg gtggccagcc
      961 aaacggtgaa gttgcttccc actcactcac ttctttgatt tttgactacg cttcaaatgg
     1021 tcaatatatt gaccaaactg aattacataa aattaatagc caaattgaat ctggaggtag
     1081 tactctcgtt ttagctcagc aattaaatga gaataaaaat tattatgata ttttctcagt
     1141 tggtgattct tcagccataa ttttagatcc aaaatttggt actctccgtg agataacaac
     1201 acgtgatgaa aattctttag gtgaagttac tcaggcaatg gggcctgatg cgggtaattc
     1261 tttttttcga caggccaatc taagttctgt tatcctaagg gagggtcaaa ctcttttatt
     1321 ggccaccgac ggttttacca gatatattga caaaggcaag tttacaccat cgcaaatatt
     1381 taaaataaaa caaacttatc ccgatgaaac caattttgtc agagctttga ttaatcaaac
     1441 aaattctctt ggtggtgtcg acaatattac tattattagt attccctata tttcagaaaa
     1501 atgaaaaaaa tatattctca aatcaaatca gctatcagtg attctcatca tattcttctc
     1561 catcttcacc ctggatctga tgctgattca gccggctccg ctctggcttt ttatcattat
     1621 ttaaccagca ttaataaaaa agtaacttta atttccggcg attccaatct atcccccggt
     1681 ctggccactc tccccggagc agaccaaatt actcctcaaa atttttttga tttaaatatc
     1741 gccgattttg atttgtttat tattttagac tcctcagaaa aaagacaaat ctctaaactc
     1801 gaagaagtgg tttttccggc gcacctaaaa actattgtca ttgaccacca tgccagtaat
     1861 cagggttttg gggatcttaa tttaattgaa gccgactctc ctgccacctg ccaaattgtt
     1921 tataaattat ttaaagcctt aaagattaaa atttctaaag aaatagcact ttgtcttttc
     1981 actggtattt tcggtgatac tggtggtttt aaatattcct cgaccactgc cgaaactttt
     2041 tcgatttgtg cgcagctcac caaaattgcg cctaatttta ccgagacact tttcaattta
     2101 gaaaacaatg accggcccga tcgtcccaag cttttaggtt tacatcttag ccatatcgaa
     2161 acacatttta acggtcgcat tgccatttct gctctccctc tgagcttact caaaaaaaat
     2221 aatatcaatc ccgatgacag tgttaatgcc gaaatatcca atactttaaa gtcggtagtc
     2281 ggttgggaaa ttggtatttc catgattgag gcgggtaaag atatggttag ggtcagtttt
     2341 cggactcgtg atccggctat ttttgacgtt agtgaaatag ccaccgctac cggctatggt
     2401 ggtggtcaca aggcggctgc cggtgcttct cttcccatgt ctctttcaaa agccaaaaga
     2461 ctaattctgc gcaccatttc ccgtctccat cccgagctcg gtaatatata ataaatttat
     2521 ggaagactgc attttttgta aaataataaa tggatctagt ccagttcaca aaatctggga
     2581 agacgacaaa catattgctt tcctaagtat ttttcctaat actccaggtt ttacagtatt
     2641 agcgactaaa aaacacttac ccagttattt tgctgacaac gatgatcaaa cactgtctga
     2701 tttcattgtg gccgctaaaa aagtcgctaa gcttattgac gctggctttg aggatgttgg
     2761 tcgtaccggt cttattgccg aaggttttgg agtcgaccat cttcatctaa aattattccc
     2821 tatgcatgga actaaatctg ataaatggca gcaacataat tcgcatataa ataatttttt
     2881 tgataaatat gaaggttata tttcctctca caatggtccc agggcagaag atgaggaatt
     2941 aaataaaata gctgctaaaa taaaaaaaca tgaccagtga acccgaaata cttcaagagc
     3001 ttcaaaaaat caatcttcgt cttgaccagt taactaatca caaaagattt tatggtcggg
     3061 aatttttagc cggtattttc cgttcccttg gttatacttt cgggactcta atcattctgg
     3121 ctattagttt ttatttttta tcaaaaatca attttaatca aatgtttact gattatataa
     3181 agcagtttat ccccaagccg gttgaaatta atgtcccttt ctctcagacc ttactcgatc
     3241 aactttagtt aaatatcttt ttcgaagcct aattgccttt ggggt
//
LOCUS       DHGB01000029            5933 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_89008, whole genome shotgun sequence.
ACCESSION   DHGB01000029 DHGB01000000
VERSION     DHGB01000029.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_89008"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1684 a         1397 c          786 g         2066 t
ORIGIN      
        1 cgattttggt aaggctatag gtgaaagctt tggtggtgtt atggccgtac ccggaaaagc
       61 cagtgacgcc cttcacagat acacaggatt ccttgacgac agagaaagaa tcatgcccaa
      121 gaaacaaaca gaaccagaag tagaaagacc aaaatctgcc tcacctggac catctggcta
      181 ttctcagcaa agaatgaaac cgaaagcatc gattcccgag gctcccgata attctggtat
      241 ataattattt tttactaaaa tatgaattac aatcaaaaaa acaagtcaaa aatatcttct
      301 tatattcctc ttgataaaaa attcaaagat cagataccac cagccccaga ttcagaaaac
      361 atataaagaa tgcctatctt ttttttccac cggatcctgt tagacgattg aatgctgccg
      421 ccccgaccat tgctttttga ccaagtagtg cctttttagg atccttaatt cctgttttta
      481 acattgctat tgcttgattg taatgctcgg ttccaatatg taaatcaaat tgagctaccg
      541 tttgcaacgc tccataaaaa ccaactccct gagcacctaa tacccccgca gtcactcttt
      601 ctaattcttc tggagtatca aaaacaccaa gggaaagaac cctccttaat tgttttgcag
      661 attctgaatt aggaacccca gacattagcg ctaatgtttt aaaatatacc gcctcaccaa
      721 ccatataacc caaaatatct gaacgacttc ctttatcagg ttgaatatta tacaaattac
      781 atagagcata gatagcttcc ccaaattttg aaaagtctga tagacctgac ccttgtgaac
      841 ctcccactaa taaatcccac accttacctt gtcttttaag atcttttact gacatttcat
      901 agggtctttt tctccccatt cctccagaat aattctcgtc agcaaattta aaaaaaccgt
      961 ctatttcatt gataatatca ggtctatttg tgaacacctg cttaaatttc aagacaccag
     1021 tcggatccat tattgtcgaa taataaacat tatcaaaagg taaaaaccca caacttgaac
     1081 tatcaggatt cgctatcctc tgggctcttg aatctgtgaa ttttttagtt ccttctttta
     1141 atcccttctg gagatatggc aaatcttcta caatgtaaaa tgacattgct atttttacca
     1201 aagatttttc tatatcatct ggaatttcat atgcagctcg atatttttgc caatctgggt
     1261 ttgagtgcaa caactccgct gttttagtta taaatgttgg aattgaggtg ggatcttttt
     1321 cttccttctc tttgtaccaa tagtatggca aaacacccct atcagcaaaa tctctaatcc
     1381 aagaaagtgg aaaaactact gccgggtcgt taagcattaa ctccgctttg tgttcatccc
     1441 aaccaagaga tatctcgtgt tcttgaggaa ttatttgctc agctaaagtt gacattgatc
     1501 catcacagtg ttgaattcca tcagcaagcc ccatacaact tacataagcc tcgattctat
     1561 cctcgtaatt aacttttatc gattcaggga ttattactcc tgctcgagcc cttgcatttt
     1621 catatgccac agaaaattca gattttaagg aactcattgc ccctccagat ctccaataat
     1681 tcatataagc acctgatcca gccaactgtg ctacaccatt ataaaatact atttgtcggt
     1741 cttttgaact tccaacatta tatagttcag ttggtggtaa gccgatccta gactcaataa
     1801 aagaaccacc ataaaaacct tttgttaaaa ttggtgatcc tcctcggtcc attgcttcag
     1861 tagccatatt attctatttt atattttatc aataattata aaattagtaa tgtttaccag
     1921 ttttctctcc ccttattaac aataaaaact tgctttttcg cctcttctct tttcatcaat
     1981 tcatattgat catattgaga catctcaatc tcagtctttt gtttgttctt tttttcactt
     2041 ttcgattttc cggttttgtc atcttccaat tcaagcctca aatatcctag tgacatttct
     2101 ttgtcaaaat tctgtccggg agttaaatta tccatatttg ccaattttcg aattgtttta
     2161 ttaaccattt cttttattcg tttctctttt atctcgtctt ccggcatcct tctcaatccc
     2221 attttactat tgtctaattc tcttagggtc tcgagcactg gaccttcttc aaaaacattc
     2281 gtcaacacta tttttcctat tcccttaaat tccttgggta aattttcgtc aattccgctc
     2341 gcattaccag ctccaattag tcccttaaat ttttcatcat ttcgaaccga gtcataaacc
     2401 acttttccca gcagatccag attattcatt gcaaattgtt tttgtctttc ggtcatgttg
     2461 ctatctctag caatttcttc caaatgttca accgacctat atccttttgc gaaggtttca
     2521 aaaaattccg ttgttttttc acttaaacta tttttatcga ctccagctgg ttcagccaat
     2581 ttcagattat taaatttttc ctttaaatca tcaaaccctt caccaatctg agttcccccg
     2641 tgtgcctcgg ccaaaaaaac actacttaca taactttcat cacccatgac aaaattatac
     2701 catctctccc ctacttattc ctcatttttt ttctttttta ggctttaggc tttagacttt
     2761 tttctttgta ataattccct cactcatcaa atctgaatac tcaattttga tcctaatcca
     2821 acagatatac gtttgagtaa atccaccgat ggattcagag atgagttctc aatttgagaa
     2881 ataattgctt gagttgtacc ggcttttttg gccaattcct tctgtgacat cttcttctcc
     2941 agccgttttt tgattatttg tcgtgacaat tgatattcaa tttctgactt ttcccaaagc
     3001 tgtttaaact caggatcctt cagactcttc ttaaaatatt cttcaaatga taaaagtttc
     3061 ataaagtaat tatatcgtat acaatataaa tatcaagata ataattcgtt taacctccct
     3121 accgcaatat taatttcttt ctttggcgtt ttatttgtct tctttttaaa cgcatgtagc
     3181 aacacaattt tttctccatc tttgttgaca tacaaaattc tggcgctttc tttccctaaa
     3241 attcgtattt cccacaacgg caatcccgat aatttcttta ggtgaggaat ggctgatatt
     3301 aatccaaatt cattgatatt tttaattatc attgtcgcct ttatttttat tcttttattt
     3361 ttatccaaaa aatctccaac cggatttctc cctcgatgat ctatgtaaaa acatacctct
     3421 aacatttgta ttaaataata ccatttataa ttaatgatta aaaattgtag atcgaagatt
     3481 gaagactttt ttcttttttt gttttagacc ttagtcttta gatttttttc tacccattga
     3541 taattgataa ttgaaaattg ataattgttt aatgtcctca attaactcta tcgatcttca
     3601 tcttcgtacc taccgcagcg ccctcaaaag caatctcgaa attactctcc actccctcac
     3661 ctcttcttac ctaaagatcg actctatttt gcatcccttt gccgccgatt ccacccacct
     3721 cgatccctcc gccctaatct attcccttct ccgtcttcct aaagatgtcg accgtgccaa
     3781 aactattgtc atgggtcaaa atccccagat ttttctcgat catggctttg gcgatgtcac
     3841 caaatggaaa aaatccactg ccaccgcccg tcgtcgcact cactattttg atctcaaaac
     3901 tggccttctt gcctcttttc tttcctctat ttccgatatt gacgatatcg tcaaccttct
     3961 cctcgcctat caaatcgaat ggaacaagtt tcatcaaatt gccacttctg cctatcccca
     4021 gtattccgaa tttaaagacg tttttattaa taatcctgag cctgtcgaag gattaaaatt
     4081 acttcaaata tcttctgaag attggcaaaa tataaagacc gcccttggca aaaattggcg
     4141 ccttcgttta aggcggattt atcaccgccc ccaaaatctt cgtcttcgtc tcctcgccag
     4201 ctcctggatc gattacacca aaaccaccca gcgttggtgg aaaaatatcg ccaccgcttt
     4261 tagcccgccg aggctttctg caaaggctag ccacatctct catcaaacaa tctactttgt
     4321 tagtagcaac gcccattccc tgttaaattt agtaaccggt ttcgcccttc agtccgaaaa
     4381 aactctttta aaactctctc ctcaaaacta ctctgagctc tctccttata ttcaccaaaa
     4441 cgattatctc tattacttat caaaatattt agtcaaagat aaaaaatatc aaagtgattg
     4501 gcaaaaaatc caaaatcgct taggtataat ttccctccct tcctccaccc acctcaacct
     4561 caatactcag attttcccgc tcaaaaattt cccaaaatca actcttttag accctcgtct
     4621 taaaatcagc aaaccatcaa aaattgccgc tagtgacgcc cttattttta atattgatta
     4681 tccacttggt tttgccgcct accatatctt aagcgagctt cttgaaaatg tttctcatct
     4741 taaaggtgtc tatattttag gtaaggccgc cgttctcaat tccgaaattg gcgatcttca
     4801 gattccccgt ctcacctttg acgaacacac tcagaattct tatgtttata aaaattgttt
     4861 caacaccttt tttccttata ccaacaccca gggttcaatt ttaacccagc aaaaatcagt
     4921 tactgtcctc ggcacctttc ttgaaaacga agctcttctc aaaaaatatt ctgaaaataa
     4981 tctgaccatt attgaaatgg agaccggccc ttttctctcc gccattaccg aggccaccca
     5041 tgatcaacaa actcctcgtg gcaccatgat tgatttaaat tccgctcctt ttgatattgg
     5101 tattatcaac tatacttccg atactcccta ttccaaggct aaaaacctcg gtgctggctc
     5161 tctcaatctc gccggcatag agccagtcta tttaggtagt ctggcaatat tgcaacgaat
     5221 aatcaatctc gaagaaaaca attaaatcta ccccaaccct cctttgaaaa aggagggaaa
     5281 aatataatcg agtatttata gtcctctccc tttttcctca aagaagagac tcggggtgat
     5341 ttaaaatttc aattcttctt ctcttttttt aattattatt tcaattttct tatcaatatc
     5401 tctgttaaat acctctctat tttctaccct aaccgttttt attcctcttt gctcaaagta
     5461 caaatctctt tcattatctg tatatttttt attgtcatga gaatcaccat ctatttcaat
     5521 cgccaataac aatttggaac aataaaaatc taatataaat tttccgactg gtttttgtct
     5581 taaaaatacg tggtcgctat ctcgtaattt tatccaaaag atcttttccg cttcggttgg
     5641 attctttcga ttattcctcg ccaacattct taattcatct agatatttca aagccccgtt
     5701 tctcattctg atctcttcag gacaaacata tttcattctc tattctatcc ccctcctttc
     5761 ctcaaaggag gggttagggg tagatttaga tttaagttat ttcctcaaag gaggggttag
     5821 gggtagattt agatttaagt tatttcctca aaggaggggt aagaggtaga tttagatttt
     5881 aatctttagg tttttttccg ttcacttctt cataaactct tcgaattctt tgt
//
LOCUS       DHGB01000030            7113 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_82408, whole genome shotgun sequence.
ACCESSION   DHGB01000030 DHGB01000000
VERSION     DHGB01000030.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7113)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_82408"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    2959..3004
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2274 a         1177 c         1453 g         2163 t
ORIGIN      
        1 cgcttcacat aaaattttga agctccaagt aatcttgaga tttttatttc atctttgcga
       61 ttatttattt tcatcccaat taaggtaaaa atcataataa aagcgataaa aatagagacg
      121 ccaattaaaa cgagacctga ctggcggatt acttttgtcc aggagagtag tgactcgatg
      181 acatcttttt gataaataat ttcgtcaacg atatcttttt tagtagaaaa agattgagaa
      241 atatcttcta atactttggg gttaaaagct gaaatctcga aagaagcggg taaaattgag
      301 gcggtgacca tttccgttaa aagcggatta tctttgttca tgtctttgta aagagaaaga
      361 gccttttctt tggaaatata acgaatttcc ttgatttcgg gaaatgaagc tagttctttt
      421 tgaatagact caaccttggc ctgatccaga ccatctttga gaaagatagt aatttctggc
      481 ttagtttcaa agtgatttaa gacactgcta aggcctcgat taattaaaag aaaaaaattt
      541 aaaataaaga agcagataaa gacagtgaca aaagcgatgg cgctttggaa gggagagcgg
      601 cgaatgtggt gaagggtaag tttaagaagt ctcatgttct gtttttccca attttataac
      661 tttttcattt aaagaatcga caaaatcggg attgtgagtc gtcatgataa tcgttgtttt
      721 taatttttta ttgatgtctt taaaaatttt taataaattc caggcattgg cggcatctaa
      781 attaccggtg ggctcgtcag ctaaaattac ctccggctca atactcagag cccgagccag
      841 gcaggccctc tggagctcac caccggaaag ctgagaaggg aaaagaaagt ggcggtgttg
      901 aagcttaacc attttgataa cttcatcgat gcgagaaggg attttatttt gggggtaatt
      961 cacaatatct aaatttaaag caatattttc ttcaatggta agatcattga tcagctgata
     1021 atcctgaaat acaacaccta ttttacggcg aattttatca atttcatttt tgcttcggag
     1081 gtgattgata gccacgccat tgattaaaac atcaccactg ctgggtttaa tttgattgag
     1141 aattagttta attaaagtcg ttttaccact gccacttgga ccggtcacaa agacaaaatc
     1201 tccgggggca atttttaggt caagatcctg aaaaacatta atgggcccgt aatttttggt
     1261 aactttttca aagactattt ccatttttta ctgtaagact ttaacactac caacatcgag
     1321 gagccagttt gtctgttttg aggcgttggt ttcacctttg acttcaactt gacggccaac
     1381 aaataaatca agatcaacca ccgatgaagt taaagaagcc cgctgagaga cgccaccttc
     1441 acgattgaga atatgagttc cgactccatt aatagaccct ttttcgatag taccggtagc
     1501 agaatcttta aaaacagtgg cggtgtctcc ataaagtttt cccacttcga ttgccgattt
     1561 gtcagtgata ttttcggtac taatggcatt ttctccaccg ttcaaaagag aggcattttt
     1621 tgagccagaa agagggtaag ttctggatat aaaataaccc attcctacgg ctataactaa
     1681 gattattagc aaaactttga cggataatag gggcgaagag ttggttttga ttagaggagt
     1741 cgctttggga gctaaaactg gggttggcga cgggtttgag gacagatttg gaatatccat
     1801 agaaccagta tagcaaaaaa ttttagagtg agtttagaga ctcagaaata aatccatgaa
     1861 gagaagcata atctttgtcc tgaggaagat aaacccattg agagttgata aatttgactg
     1921 gatgataaat taaattcgtt tttggatcct caccaatact gattggatgt ctgaggattt
     1981 cgatactttt tagtttttgg ctgtatctca atagatatga aattaactca gtatcagtaa
     2041 agttatgttc taaagtaccc caaaattgat aaagtttaaa aataatttga gggtttttgg
     2101 ccagagaact ttttaatttg tcaattaatg tattaatcaa aagttgttga cgctcgatac
     2161 gaccgagatc agtccctcca ttggcagcag tatcggcact ttttcgggag cgaacaaatt
     2221 cgctaatatt gcggctatca agatggttcc aaccactttt aaattctaca gtccgataaa
     2281 cgggagcatc agaatcagga ttaagaatat aggcctcgtt tgggtatagt tcgtccttaa
     2341 aacccctttc taaatacaaa tcaactccac cgagaatatc agccagctct tttaagtttt
     2401 ctgtagataa aattaaaacc tgattaatag gctgaccgga aatcgaagca aagttttggg
     2461 aaatatattc aaatttttcg attgaattat ttttatctaa agataaggga taaatttgat
     2521 ttattttagt attggtgtaa tagctccaaa gatcccgagg tagggaaaat agatgaactt
     2581 ttgcttgctt taaatctaac tccgaaaaaa taatcgtgtc ggtcgtctcg gttttttcaa
     2641 gccaatcatc tcttttatca actcctaaaa taataaaatt aggtttgtca gtagtgggag
     2701 taaatttcaa ggcactaata atagttttgg ggaaatagag aaaaggtcga ttagtttttt
     2761 ttgataaata aataaaaaat attattaaga taaatagaag tgaaaagata gttaatagaa
     2821 atatttttaa aaatttgttc ataattttag ttcggccgta ataaaatcgt caaggtcgcc
     2881 atctaaaaca gccgtggtat tggaagtttc gtggcgggtg cgcaaatctt ttaccatttt
     2941 atagggatgg aggacatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnncatggc aatcagccgg ttttgatctt gagagcgttg actctgacag gcgacaacga
     3061 tgccggtagg cttgtgaaca attctgacgg cggtagaaac tttgttaaca ttttgaccac
     3121 cgtgaccacc ggcccggtaa gccgcaaatt caatatcttc aggacggata ttaacttctt
     3181 tgggatcttc aatgaccgga gaaacttcga ccttggcgaa agaagtttga cgaagctgat
     3241 cggcattaaa gggtgaaagg cggacaagac gatgaacccc atcctctcgt cgaagatagc
     3301 cgtaagcata aggagtacta attttaaaat agactgattt tatgccggct tcctctccgg
     3361 gagtaatatc gaggagttca tatcgccagc cctgacgatc aaaatagcgt agatacatcc
     3421 gctgtaggat tgaagcccag tccatggctt cggtgccacc ctgaccggag tggattgaga
     3481 gaatagcctg atttttatca tatttacctg agagataggt tttaagttct aaaacatcaa
     3541 tatttctttc aagtaattta attttcgatt ctagctctaa tgtcagggaa gcatccggct
     3601 cagtttcaag catggtggaa aactcctgta aatcggtgat ttctttatca agactattga
     3661 ttgtagtaat agttttctgt aggtcagaaa gcgattgcat aatgtccgac gatttttgcg
     3721 gattttgcca aagagagggg tcctcggatt gagttttaag atctttctcc tgcataacca
     3781 aatcagcata atttaatttg tttttgatcg cttctagttt ttgatggagt gaaattaaat
     3841 tcatttttta ccaccctcga tgctttttat aatatcttcg tagactttgg taataatttg
     3901 tttttttaaa tcgacatatt ggtcgttaat aaagttgata acttgatttt tgtattgttc
     3961 aatggttttg gtggcttcat ttaccaatgg ctccgtgctg actccggcaa tatttagatt
     4021 agatgaatag ctggaaagag gagaagatga atggttatta gataaatagt ttatagagaa
     4081 ataaataatt agagcaaaaa taagccagcg aaataaaaca ccgagattaa aatgaaattt
     4141 ttttcttttt gattttgagg acacaaataa attgtatcac ctactttcta gccactcgat
     4201 aatgggggtg tcgccataaa gacagacact attttgggta tcaaaagcca ggggaacccc
     4261 gaccccctga ctagaatcaa tctcgggaca ctttttgact gtttcctcga gctgtaactg
     4321 attatcttgg ttgtaatata cttctttgct cgctattttt actttggact ccaaattatt
     4381 ttcatttata taatctttta ctttttcaca atgaggacaa ccttcacccc aaaaaagaat
     4441 taactgcgcc gaggcatcgt caacttttga gccaggaaca tcagtttttt gaaaattaaa
     4501 gtaaaaataa gcagctgcga caacaataat taaaataagg ggaattaatt ttttcattta
     4561 gtggttgagc taatttttaa tattttgtca ccttgaacaa ttttgtccag aacttcaaga
     4621 ccagaaataa cttcaccaaa attaacgtaa tctccggtta aatgctgaca accttcgtcg
     4681 gtaaagcaga taaaaaattg gctatcgtta ctaatttctt ttgaggctgg agttcgggcc
     4741 aaaccaaggg aacctctttt aaaaggaatc tgatttaatt ctgatttttg agaatctccg
     4801 ccagtaccag taccagtagg atcaccaccc tgagccataa aaccaggaat gacccggtga
     4861 aaaataagac catcgtaaaa accactgtta acttttttaa gaaaagtcgt aaccgtgttt
     4921 ggggctttat cagcgtaaag cttgataaca atctgaccat tgatagtgtc cagagcgaca
     4981 acagtctcag tattaacgac agctgattgg gttgatgtgt tttcttcaac aattggattt
     5041 tgaggggtca tagtgggtgt aggtaaaatt tgagttgaat tattaagtga acaagaagag
     5101 agaaatatta aagaaaataa gataactaaa aatttcattg cttattttat cagaactaga
     5161 gctctaggaa taccctgatt gatatcttct tttttgaggg aggggggaaa gtcgtgaatg
     5221 gaatccatga gagtggcata agaatagcga tagtaggccc caaactgagt cccgacgtca
     5281 tgaactataa actgattttt ttcgtcatca tagcccagaa taattaaatt gtgataccag
     5341 ggaccaccac ttttgaaatg tttattttct ttatagagag ttttgccatc gctcggaata
     5401 ataatgggaa tatcttttgc cagatactgc ttaaagtcgg aaatagtcgg attttctata
     5461 attctgtttt gataattcca aagctcggcc gagagagtcg ccatttgagt aagggtaata
     5521 tcgtgagtcc agccaagatc tgattctaaa gaaaatattt tttgataatc tgcaagcatg
     5581 gtagcaatat ctgcctcctt ggattcgtaa taataatgga cggtaagtaa ggcagattct
     5641 tcacaggcat cctgccaggg ctgatcccat tttttttctg gggcctgagg aacaaaggta
     5701 gttttaatga gatgtttatt tggtaaacca ttttccttaa taatggtcgg agcaggtaaa
     5761 atgttttcga caataatttc cgggggaggt atttgatggc gatatttata aataaaaatg
     5821 gaagacaccg atataattag caatattaac aaaaagcgaa tatacttaaa catagttagt
     5881 attttagtgg taaaatcgac ggatgcaaat agccagtgat atcagaaata tagccgtaat
     5941 tgcccatgtt gatcatggca agacgacatt ggtagatgcc atgctaaagc agacccattc
     6001 attcagggag aatcaggatg aaatgaaaat ggagagaatt atggatagca atgatttgga
     6061 gagagaaaaa ggaattacga ttttgtcaaa aaatacctcc gttttctata aaggaactaa
     6121 aattaatatt atcgatactc cgggacatgc tgattttggc ggagaagtgg aaagagtttt
     6181 aaacatggct gatggagtcc tgctcatagt tgacgcagcc gagggtccac tctcacaaac
     6241 taaatttgtc ttaaaaaaag ctttggatta taacttaaaa gtaattttag taatcaataa
     6301 aattgaccga aaagatcaaa gagtcaaaga agtagtggcc gacactgaaa atttattttt
     6361 atcgctggct tctaatgaag atttgctcga ttttccggta atctatgcta tcggtcgcga
     6421 aggtaaaaca ttttatgata tgcccgataa tatggatata agtggtgata cgacccccct
     6481 atttgaaaca attttaaaat atataccctc cgcgcgaaaa gatgatgctg ccccttttca
     6541 aatgctggta tcggcttttg aaaaaaatga gtatttaggc agactggccc tcggtcgaat
     6601 tcaccagggt aaaataaaaa aaggcagttt tgtctccctg attagacctg atcaaaaatc
     6661 tctcggaaat tttaaagttg agagaatgta cgttaacgaa ggcataaata aagttgaggc
     6721 caaggaagcg ttaagtggag aaatagttta tctggctggg atgcctcaaa ttgaaattgg
     6781 tcaaagtatt acggatatta attaccaggt cgccctaccc atgatcgaaa taactcagcc
     6841 aactctaaaa gctagttttg gcccaaacac ctctgtcttt gccaaagaag aatcaagatt
     6901 tatgaccagt cgagaattaa aagaaagact ccttgaagag gcagaaacta atttaggtct
     6961 aaaggtcgaa gatgatccgg ttgatagcat ccgaatgata gtgtctggga gaggtgaatt
     7021 gcatttggcg gtcctaattg aaaaactccg acgtgaaggc tatgcgatgg aaattggaaa
     7081 accggaagtt attttgaaac aagttgacgg caa
//
LOCUS       DHGB01000031            2894 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_152907, whole genome shotgun sequence.
ACCESSION   DHGB01000031 DHGB01000000
VERSION     DHGB01000031.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_152907"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT          890 a          568 c          532 g          904 t
ORIGIN      
        1 tgttatttag gtttcgacgg tattgatatc aattttggtt gccctgccaa aaccgtgact
       61 cagcatggct ccggcgcctc tcttatcgat aaacccatgc tagccagtca aattatcaaa
      121 agcgttcaaa aagctgtttc tgattttgcc aataaaaaag taactttaaa agacttaaaa
      181 ttaaaagaaa aagtattaaa agttattgaa agaaatcaaa aatattcggg tatagagacg
      241 ttgcatgcaa cgtctctatc aaaaaaaccc accgtttctg tcaaaactcg tcttggcatt
      301 atcaccgaca tctcagatac ttggattccc tttttactct ctcatcattt agattttttg
      361 actcttcatg gtcggaccct aaaacagggt tatggtggtc ttgccaattg ggacgttatt
      421 gccaaagccg ccaaatatgc ccaaaaaacc aagactatat ttttcggtaa tggcgatgtt
      481 cagtctcgct tggaggcctt agaatactgt caaaaatatg gagtaaatgg ggttttaatt
      541 ggccgtgccg ctatgggtaa tccctgggca tttaacgata aaaaacccga ctttaaggaa
      601 aaatttaacg ctatgctttt acacgccaat aattttactg ccatttttcc ttcccgccgt
      661 tttgatccat taagacgtca ttttcttctc tatgtttccg gtcatcccca tgccaagcag
      721 cttcgtcggg aaattgtcaa attgactacg attgaccagc tttatgctct tgaagagcgg
      781 attactaatt ggtaaattaa acagtatggc cgattcccag aatcttgctt caaatttaat
      841 tagtattgaa tcattaatca aaagtcatga tcagcgtctt gatactttaa ccaaagaact
      901 tagaactcaa aaagatatgc tcgatactat tttagataac aacgaggaat atcgcctcac
      961 tgcgaccgag gccagtaaac aggcaaaatt aaaaactctg gctaaacaaa aagtcttaaa
     1021 aagcccggag tctatggctc ttgttgaaaa aataaaagat taccaagctc aaattaaaga
     1081 actaaaagtg gctctttccg attatttggc tcaatacgtc actatttccg gttctaatca
     1141 aatcgagggt tctgatgggg ttcttcgaac cattgtctat accgctaaat tagttaaaaa
     1201 aactgattaa aaaaatatgc ctcttactgt caatcaagat agttttcaga cagaagtttt
     1261 agattttaac ggcaaagttc tcgttgattt ctgggcttcc tggtgtggtc cctgccaaat
     1321 gcttggtccc atcattgatg aaattggtga cgaatacaaa gacaaagtga aagtagttaa
     1381 aatcgatgtt gacgaaaatc agaatttggc tcttcagtac aatatctctt ctattccggc
     1441 tgttttaatt ttcgatcagg gaaaagttat tgagaccatc attggttttc atcaaaaaca
     1501 ggattattta aaagcgttag aataacttgt ggatgtcaag gcggtacaaa gctatctttc
     1561 ctctttaaat ttaccccaat atcggcaaaa acaaatttta aagaattatt tctctggcca
     1621 ttacgctacc tttttagaca tgaccgatct tcccaaagat ctgcgccttg atttagaaaa
     1681 aaacttttct ctgctttcgg ttaaagagtc gaaaataatt atcgatacgg atactcaaaa
     1741 agccctttta actttaaaag acaatttagc cattgaatca gtcttaatgg attatgatga
     1801 ctggctaacc gcctgtcttt cctcccaagt tggctgtccc ctaaactgcg ccttttgtgc
     1861 caccggcaaa atgggtttta tgcgcaattt aacggctgaa gaaatagtcg atcaaattct
     1921 ttactggaaa aatattttat caaatccaaa taagcctcct ttcctcaaag gaggtgcccg
     1981 gagggcggag gatttaaagc gccttgtttt tatgggtatg ggcgagccct ttttaaactg
     2041 ggacaacctt ctggtcacct taaaaattat taaagaaaat ctaaatatcg gctcccgaaa
     2101 aatctcgatt tctaccgccg gtattattcc caaaatttat gaatttgctg atcttaacac
     2161 tgaaattaat ttagccatat ccctccatgc ccccgatcag gctactcgcc aaaaaataat
     2221 gcccatcgcc cgagagtatc attttgacga tttaattaaa tcactaaatt actacacggc
     2281 ccatacccgc cgtcagttat ttttagaata tgctctcata gatggtatca atgatagcga
     2341 gcttcattta aataaattaa tcactctttt aaaaagtaac cgtttatttt atttaaattt
     2401 aattccctta aacccggtaa ggggtggttt aaattcttct tctaaaataa atttattttc
     2461 tgacaccttc aataaaaatc atctaaatta ttctcttcgt cgatctctcg gtcagtcgac
     2521 taattctgcc tgtggccaat taattgttgg aaattagggt ataatcaaaa tatgttaatc
     2581 gatgaagttc ggattgttat taaatcgggt aatggcggtg atggtttggc ccatctttac
     2641 tctgatggtt ctcggccaaa gggtggtccg gatgggggca agggtgggga tggtggcgac
     2701 gtttatttta aagctgtctc ggacattagt cgtttaaatc agtttcgttt tcctaaaaaa
     2761 tttatggctg aggatggtca gaaaggtggc cagaataatc ggagtggtgc taatggcaaa
     2821 gacttaattc tcgaagtgcc cgttggtacc gtcattaatt atgacaatgg taccagtaag
     2881 gaattaaccg aaat
//
LOCUS       DHGB01000032           17563 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_36510, whole genome shotgun sequence.
ACCESSION   DHGB01000032 DHGB01000000
VERSION     DHGB01000032.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 17563)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17563)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17563
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_36510"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         5013 a         3516 c         2905 g         6129 t
ORIGIN      
        1 aaaatcatca aaattaattg agaaatcatc cagatgtggt ccgcacttac tatgtcctgc
       61 cgctaattct tgtggatata gttgacgtat ttttccttcg tttatggttg acgcttgata
      121 tttaattgat aatttattaa tttccttatc ttgatggtta gtaaaaaata aattagctga
      181 tttaacccag gcaaagcgga aactttgaat aatttccgca ttttttataa gtgattgatc
      241 ccagtaaaat aactccgttt tttgagcctt accactggca ataatatcaa gtagttcatt
      301 tcgatttttt agagccttgt ggtactgaga gagagccgag gcgtagcgcc attccgtatt
      361 ggcaaaaatt ccatcaaaaa agtctcttct tctccctggt gaaccattaa ttagtctcaa
      421 gtcttctggt tcaaatacca ctatttttac cttcccccag tatttagccc tagttttgac
      481 tactttatca atcaaaaatc gtcgttttaa ggctccatta tcttctcgcc agagttgaac
      541 ctcaacttca tattcccctt gtttgtccaa tattttagct ctaacgctac taattttctc
      601 tccccatttt attaattggc cagagcttga cgatctaaaa ctttttcctg aagacaaaaa
      661 ataaatcgct tccagcaaat tactctttcc gctcccattt ttccccaaaa ctaagtttgc
      721 ccctttatca aattccacca actttttatc gtatgaccga aaattctgta ataatatctt
      781 actaatatac atcttccgct tattatagcc gattcttacc taaaattctt tgttttatta
      841 aattaaaaac aaaaatttat ctctattatt ttttcaggta gtttagttag tactatcatt
      901 gaccaataaa ttaaaagctg acaaaaaacc catatcttta atttcattat caataatcat
      961 tttttttatt ttatctacat caattagctc gtagctcaat atttcatctt cttctcgttt
     1021 ttcctctact ttttcatcct gaatttttaa aattaccacg tatgctttca tcgaacaaaa
     1081 tccgggctgg gggtatattt caaaaacttt gctaccaact ccaataaaac cagtttcttc
     1141 taaaagttct ctttttgccg catcaagcgg tgtttcatct ttatttatcc agcctgctgg
     1201 gagttcataa acaaaatcat caacacctct tctgtattgt ctttccacga taattttttt
     1261 gtccttgtct atcgctaaaa ttaacacata atcaggtcta tttagatggt aatacccttc
     1321 aacaattttg ccatcaggta gttcgtatga tctatcttca attgataacc atttggaatc
     1381 gaaaacattt ttcttgccca gcaatttcca ttttttatca gtcataaatc attttaacaa
     1441 aatatcgaaa atggtttatt gctttttaaa agagatgtgt ccagctggta ggagttgaac
     1501 ctacttaaaa cggttttaca gaccgctgcc ttaccgtccg gcaccagctg gttgtcgtga
     1561 gtgattatag caaaattatc aggtaaaata gaatcactta tgactcttcg tctcatttct
     1621 tggaacctcg ccggtatgcg tgcggctatc aaaaaaggtc tttttgattt tatggaaaaa
     1681 gacaaggcgg atatttactg ttttcaagaa gttaaggcca aagctgacca agtccctctt
     1741 ctagattgtc caaggggtta tcgtcagttt tggaaccctg cggccaaagc cggttattcc
     1801 ggagtggcca cttttactaa aatagaacca aaatctgtca ttatcggcga ccgcgataac
     1861 gattgggatg atgagggacg agttatcatt accaaatttg acgaatttac tttacttaat
     1921 gtctattttc ccaacggtca acgagacctc ggtcgcctcg attttaaaat gaaattttat
     1981 aattattttt taaaatatat cgacgacctg cgagatagag gagagaaggt aattttttgc
     2041 ggcgatgtca acaccgctca tactgaaatt gatttagctc gccccaaaga caacgctaaa
     2101 acttccggct tcttgccgat agaaagagcc tggatggata aattagttgc tcataattgg
     2161 actgatacat accgcctcat taataataac aaaatcgaat actcctggtg gtcccagcgc
     2221 tctggcgccc gtcccaaaaa cgttggctgg cgcattgatt atttctttat tgataaaaaa
     2281 ctagagtcca aaattaccga cgcttttatt ctcactgatg tctacggctc cgaccactgt
     2341 ccgattggaa ttgagattaa attataaatt tatttgttcc ttcccttttc agccaccgat
     2401 tattttccct ccagttactg tcgtattttt ccagaagttc ccaaaaagct ctcgaatgat
     2461 ttttgatcgt taggtgggtc agttcgtgga gaattacgtg ttcaaattta tccatcggga
     2521 ataaaattat ttgccaattg aaatttaatg agcccatccc agaacaggat ccatatcttg
     2581 agctttgatt ttttattccc actttttcgt agtgtaaatt atatttatta gccagctctc
     2641 tcgttttttc tttaattaat ttaccagcca gaattctcat ttttttatca aaaatttctt
     2701 ttaatttatt tttttccgaa tagtttatca agatattttt atcgataatt accagtgaat
     2761 ccctcaaagc tttttttgtc tctacttcgt aattttcgcc taaaatttct atctttttaa
     2821 ttttatttaa tctttttttc ttttttattt ttttattttg tcttagaatc cagccttcat
     2881 tgttcttaat aaatttattg acgaccatca aaggcatcag ccaggggata ttaaccacta
     2941 ttttattttt tcctactatt ctcagtcgta atgtctgcac ccttttcctc tttatttcta
     3001 ttaaaaattt tttctctcca atcttaattt tttccataaa tgttaatata gtataaggga
     3061 tgatatatat tgctgctgac catcgcggtt ttcggttaaa agaagagata aaaaaatggc
     3121 tcgaaatcga gctttatgaa tttcgtgatc tgggtgctac tgaatacgaa cgtgatgatg
     3181 attatccgaa gtttgccata aaactaggtg aaacggttac cagagaaaac gctcggggaa
     3241 ttttaatctg tggctccggg gttggtgctt ctatcgctgc taataaggtg agtgggattc
     3301 gcgccggact ttgcacctcg gtcaaacagg ctgcctccgc tcgaagcgat gacaatatca
     3361 atattctttg cttaagcgcc gactgggtaa gcgtcaaaga taatcaaaaa attactgaaa
     3421 cttttttgaa aactcttttc tcaagcgagg agcgtcatat tagaagaatt aatcaaatca
     3481 aagactatga aactcaagag cgttgaagaa attattcctc aaattagggt tgttttacgt
     3541 ctcgatctcg accttccttt ttttgataat aaaattattg acaactctcg tctccaaaaa
     3601 tctttaccca ccattagatt gttgctcgaa aaaaaatgca aaattttaat tatcggtcat
     3661 cgtggtcggc cgaatggcat cgaagacaaa tataccctca ggccagttta cttagaacta
     3721 atgtcccttt tagaaccaga aggagagtct ttaattgaaa gtgtttttat gccggaaatt
     3781 aattttgaac aaattgatca ggccttggct gtcaataatc ttgttttttt agaaaatatc
     3841 cgtttttggc cagaagaaga aaataacact gcctcttttt tagcccccct cagtggattt
     3901 tgtcaggcct atgtcaacga tgcctttgct gtggctcatc gcgctcacgc ctcgattact
     3961 ttatggcaac atttacccag tttttatggt ttaagcttta ttgaggaata taatcacctt
     4021 gtctctttag gaaatccctc ccggccactc acccttattc tcggcggtgc caaagaggat
     4081 aaattaaaac atctggataa attaatttct cactgcgatc acattttaat tggtggcaag
     4141 ttacctcagc ttttatccct cgttgacccc aaaattattc ctgctatttt gactccggat
     4201 acctttgata ttaatgaaga atccatcgct aaattcactg aaataataaa taattcaaaa
     4261 acaataattt gggcaggagc tatgggcttt tatgaaaatg aaaattatcg aaacggaact
     4321 gaaaaaattg ctcgggccat tgccgctacc acagcctaca aggttgtggc tggtggtgat
     4381 acctccgcct ctgttttaaa tttaaatctc aaagataaat acgatttttt ctgtagtggc
     4441 gggggagtcc ttcttgaatt tttggtcaat cccaatctcc ccgcctggtc gaattaacag
     4501 attcttttgt tgtattatta attatggaaa caaaaaaact gaaaatcggc attaatggtt
     4561 ttgggcgaat tggcactgtc gttcttagag ctattttgat ggaaaaatat gacgttgaag
     4621 tggcggcgat taatactcgg ggtgagcttg aaatggaagc ctacgcctat cagtttaaat
     4681 acgattctgt ctacggtcgt tttcccggta aaatctcttt tgccgaaccc caaaaagccc
     4741 gtgaaaccgg ccgtcttctc atcaatgatt tagaaattcc ctttctagga gaaagtgacc
     4801 ccgctcaaat tccctggaaa aattatgggg ttgatatcgt tttagaatgc actggtgcct
     4861 ttatcgactc caaagccgcc cttcatttaa acggtggagc caaaaaagtt attatctctg
     4921 cccccgccaa agatcccaag attccggttt atattttggg tgttaatgct gataaatacc
     4981 atggcgagac tcttatttcc aatggctcct gtactacgaa ctgtgtcgct cccattgtca
     5041 aattaattga tcaaaaaatc ggttttcagg agggtgtttt gaccactatt catgcctata
     5101 ctaccaatca aaatattgtc gatggcagcg gtaaagatcc tcgccgcgcc cgagccgccg
     5161 ccatcaatat tgttcccact tctaccggag cggccgaggc cgttattgcc gcctatcccg
     5221 aggctagggg gcgttttgct ggtaccgcca ttcgggtccc tgttgtttgc ggttcttatt
     5281 ctgatttaac ttttaaatta gtccgctcct ctactgtcga agaaattaat gctctttttg
     5341 aagatgctgc ttctcaatct tccctcgtcg ggaaattaaa agtttcttat gagccactcg
     5401 tttcttcaga tattataggc aacaattctt catgtcttat cgatactcgt atgactaagg
     5461 tcgtttccga cgatatggtt tctatcggag cctggtatga caatgaatac ggttatagct
     5521 gtcgtctcat cgagatggct cttcatatat ggaaataatt cccactattt tagaaaaaga
     5581 tttcgattta gctgaaaacc gtttctctca aataaaaaat ctctcttcct ggatccaaat
     5641 tgatgttgtt gataatgttt ttacctctgg aaaaagtttc gagctcgaat taatttcaaa
     5701 atttaatcaa agatactcta gtctatggga tatccatctg atggttaaag aaccgattaa
     5761 ttggctaaaa aaatgtctct ttgttgatgc ttctcgaatt tatggtcaag ttgaaatgat
     5821 gtcaaatcga gaaatctttg ttactgaagc caaaaattta ggtttagagg ttggcctggc
     5881 ttttaatatt gataccccgg ttgataatat tcccagtgag tgcgatttaa tcttactaat
     5941 ggcacgtcca gccggctttg gcacctatcc tttagatgaa actatttttc aaaaaataac
     6001 tgaagctaaa aaaattggca aaaaaattgc cattgatggg ggagttactc cagataattt
     6061 ttctcaaata aaacaattcg gagttgatat tatttattta ggtcaatatt ttttagattt
     6121 aattcatgaa aaagaaaaat aaaaaacgtc ttcgcattgc ttttttcggc aactctgatg
     6181 tcaaaaaaag aagcctcagt tttattgatg cctatgatac ggctcaactt ttaagtaaaa
     6241 acaactttac tattatcaat ggtggtgggc cgggaattat gctggcttca acccttgggg
     6301 cagtttctgt tcacggccga gccgaagctg tggttatcaa tccaaagagc caaccagtcg
     6361 ctcattacga aggccagtat ccggagaata tttctcaggt taaacgaatc tattctttaa
     6421 aatcttatca aggaagactg agtaaactaa ttaaacttgc tagtgcttac gttatttttt
     6481 acggtggtac cggcactctg gccgaaatgg cttatgtctg gtctgaggcc aaattcgcct
     6541 ttccccgcca aaaacccatt attttttatg gcaaaaaatg gcgcaaaata attaatacca
     6601 ttacttccga attaaaattt gaaaaaattg aaaaacaaat ttgttatttc gtcgacaaac
     6661 ccgagcaagt tttggaaatt atcaaaacct attccaaata atcttgtagc ttttttcttc
     6721 gacttggatg tcttaatttt cttaaagctt tggcctctat ttgtctaatt ctttccctgg
     6781 tcacattaaa aattttacct acttcttcaa gtgtttttgg ggtttctccc attagtccaa
     6841 aacgtagtga aagtactttt gcctctcttt cgtcaagact ttcgagaacc tcttcaatcg
     6901 actctcttag gagctctttt gatgtttcct cataggggct tggctgggtc aaatcttcaa
     6961 taaaatctcc cagaaatgaa tcctcatcat cgccgactgg ggtggcgaga gaggttgtat
     7021 cttgagatat tcggtaaatt tcttcgactt tttctactgt aattcccact tctgcggcaa
     7081 tttcttcgat agtcggttct cgtcccagtc tttgggtcaa ggctcttgtc gctttataaa
     7141 ctttattaat tgtttcaacc atgtgaaccg gaattcttat tgttttagcc tgatcggcaa
     7201 tggcccgggt gatagcctgt cttatccacc aggtcgcata agttgaaaac ttaaaacctc
     7261 gtttccaatc gaatttttct accgctctca tcaacccctg atttccttcc tgaactaggt
     7321 cgagaaaggt cagacctctt ccaatatatt ttttcgcaat tgacaccacc agtctcaaat
     7381 tagcattgat tagatgagct ctggcctctc cggagccttt ttcaattgcc ttagctaatt
     7441 ccacttcttt ctcaaaatta attaaatcaa tttttccaat ttcttttaaa tacattcgca
     7501 ctgagtccat gctatttttt tgagataaaa gtgccaaaag atcggcactg ctcagctttc
     7561 ccttttctga atcaattatc tctcttgact ccaccttgtc aaaaatatct attcccttat
     7621 cgaacagata atcgaaaagt tgatcaattt gttcaatata tttttctggt tcggggatta
     7681 gggctagaat ttcatcatta gtaagatatc ggagtttttt tgcctgagcc actaacttag
     7741 tttttatttg ggatatgttt attgttgtgg ccataatttt gtattctatc aaaaattagc
     7801 cctcttaaga cttagctctt tgcaatattg ccagttttgt caatatttga gcgtaatttg
     7861 cctcaacttt ggcaatttcc ttttcgtctc cagaggcctc tagattagca attatatcac
     7921 ttaatgattt tagcctgtcc tttaactcta gttcttctat ttgcctaagt attttactcc
     7981 tttcttttct cctttcttca gcactatttt gatcttcaaa agcttttaaa tataaattat
     8041 taaaagtgtc aaggagttct gggtccaaat tttccgccac ctttttagct tcaaattttg
     8101 ttatcttttc tatcactcct ccaattttta taaatcgtag cgttttccac tttagcagtt
     8161 tgattgctat ttttggtttt tgcagcctca aagccagaat taaatacctt tcttccagcc
     8221 tttcccgcat atctcctttt tcttcaattt tatttatttt ctttaaattt gaaacacttt
     8281 ctttattttg atttaaatat tttttgaatt cttcagccac actttcttca tcagaatcta
     8341 tagtctgaga gacttttttt atataatctg ccctaattac cagattatta attttggcga
     8401 gaaagggcag gacagttgct aaaattaatt ttttacccct tgggctatct ataccatagt
     8461 ttttaacact agaggctaat ataaaatccc aaattgtttc tgcttcattg agcctttttt
     8521 tgaaaaaatc taaatcttta tttattgcct catccggatc tttatatttt tctccaagat
     8581 ccaggatttt tatctcaaaa tccattttat cagccagctc aatacttcta atcatggcct
     8641 tattaccggc aaaatcggaa tcaagtccca gaattaaggt ctcagtgtag cgtctgatta
     8701 attggagctg ctcctcagtt aaggctgtcc cctttatggc caccacgttt tcgattcccg
     8761 cctgatatgg cgaaatcata tcgaactctc cctcgacaat tatggctaaa ttattttttt
     8821 taatactttc tttggccaga ttcagtccat aaaacatttg gcttttgtgg taaatttcag
     8881 tttccggcga attaatatat tttgctaaat tctgattttt attactgggt aagattcttc
     8941 cggaaaatcc cacaattctg tcccgaaaat caatctgagg gaaaattagc ctatcgctaa
     9001 aacggtcata actttgacct tgtttatact gacttttccc aaaagaacct gttttatata
     9061 attcttcggt tttatagccc tttttaagta ggtaattgat aattaagcgg ctatcgggtg
     9121 gggcgaagcc aatcaaaaac ttatttatcg tttcctcgct aatatttctc tttttgatat
     9181 attcaagtgc ctctttacca agctcatgtt ttaataaaat atagttataa aatttagctg
     9241 tttctctgtt gatttcaaat aagcgttttt ttattgattc ttcaccactg attttagtcg
     9301 accgcactag tttaattccg gccatttttg ccaaatcttc aagcgcatct ttaaaatcaa
     9361 ttcggttgta ttcctgaaca aaagtaaaaa catctcctgc cttgccacag ccaaaacact
     9421 tgtaaatttg cagttccggt gagaccacaa aagaaggggt tttttcttga tgaaaggggc
     9481 aacaagccac gtagtgacga cctctttttt tcaaaggcac aaatttatta attacttcta
     9541 caatatcgag cttcttttta atttcttcaa cctgattttc cactggccta ttctaccatt
     9601 tgagctccta tctctagtaa ttcctgttta cgataaaatc tatgcctgat attagttgac
     9661 ttcggtatgg ctctctttta ataaaacgta gttctttctc aaatattatt tttgcctgcc
     9721 ttgcatattt atcagccttt tctttactgt agtcaagact tccgtatttt ttcattaagt
     9781 caacaataac ctcaacatcc tgttcagttt tttcgtttct ttttttattt aatatattac
     9841 taattaactt cttatctctt tcactacttt tctttattag atctagtaac atcagggttc
     9901 ttttattttc ataaatatcg ttccccattt ttttttgacc tgaaaaatca gaagttaggt
     9961 ctaaaagatc atcaacaatt tgaaaagatc ttcctaaaag tactccaaat cgataaattg
    10021 tttcaagttg tttttccgaa gctccggcga taatggctcc tagcctcatt ggtccggcaa
    10081 tggtgtaata tccagtttta ctttctagta ttaataaatt atctttttca cttaaatcat
    10141 aacgattatc cctcgtccat tttaattcta tcccttgacc aagtatggtt cggctcatca
    10201 tggtataaaa ttcttcgaca attttcatcg ccgttttttt atctaatatt aagaaattat
    10261 cagaaagaac tttccacata attacgtgta gggcatctcc ggcattaatg gccagctcgt
    10321 ttccatagat tttatgaagg gctttttgac cccttcgact ttcagaatca tcttcaatgt
    10381 catcatgatt caaaatccag tcttcagata tttgcatggc cgcagcggtt gtcatcgccc
    10441 tgtctctttt acctcccatc ccttcagctg tcagtagtag caaacttggt ctaagatatt
    10501 taccctttct tcgtggataa tcactcacaa ctttgaggtg aaaatcaagt tccttttgat
    10561 attttctatc tagacgacaa taatcaggaa atatatttac ttcttcaatt ttcttttcaa
    10621 taattggcca aagcaaatcc cggtaacgat ttaaggtatt taaaaaatct tggctttcca
    10681 tttatgattc agtcgttacc aaatctgtat tctcttcagt ttttcttcct tggtttgctc
    10741 ctgctctaat cagtattttg tcaaaagtat agggggacaa aatataaagg gcctcaaaag
    10801 ttgctaccga taattcaaaa gcgcagggaa tctggttggt gatgagaaag gctcccagac
    10861 ctaaaaacaa tgcatttctg aaagtttctt tcatcttgtc tttaaatacc ttatcgagct
    10921 ttatttcatc aacacctctt ttttccgcaa tttccttatc gatgttgtgt ctggtatagc
    10981 cagcatgaac agtggtcaag aaggttccca cggccgtaat aaaattcata gttgtattct
    11041 gtctagttaa atccgctaaa agtagccaat tttcaaattt taccgggaaa tttctaagca
    11101 gtattatcga caaaatcaag acagtcccgc taaagcctcc actttggagc agaagccgat
    11161 tatagttaat ccttagcgcc agtatttttt tattatgtgt taaaaaagct ctgaaaattc
    11221 tccaaagtag ttttaggact gcaaaaaata aagaaattcc aagccagatg aagagtattg
    11281 ttttccaatc cacactaatt gtttctgtta ttttaaaaaa tgggagttta aaaatagtcc
    11341 ccacgagcgg gtaatcggga tacggtaccc atttataaag cagggcggga tctagtttct
    11401 gggtataaaa gtcaattaaa aatattcttc ccgccgagaa aaaagcaatt gcattaacaa
    11461 taaaatttaa aatataaaat aaaattccca agtcgccctt ttttactttg tcagtaaata
    11521 ccaaataagc atcatcatta ctactttttt cgatttcttc ttcaaaatct ttaaaagact
    11581 gtgacagtct catgccaata aaacgcatgg gcaaaaggac aatattttga catataccta
    11641 ttaaaagcac tgaaacccag atttggccag ttaaatagta aaggctaaaa aaagcactgg
    11701 catagagtcg aaaactaccg cctttaaaaa accaaactgc gattactacc aaaaaaatca
    11761 ctaaaaatgt cgttgccatc ggcattaata ggcgactgtt gtcaattttt ctaatctctc
    11821 taagtgttga tgccatattc ctgttggcat tatagcaact ttagcctata cttttataat
    11881 gcctgaaatt aaagagaaaa aaccggaaat atatccaaat actgcagaaa acaaggcagg
    11941 aatggttgat atcggccaag aaaacattgg tcgagaacca gttcctcggg aaataaaaac
    12001 ctggatggaa aaagtcgaac aggtcacaac ttctgggcca aataccatta atgatgatgg
    12061 acagattaca ggggcctcct caaattctca agaacctcaa attacccttc cgattactaa
    12121 aaaaactttt atagttggct ttaagaagac ctggcaagaa gcgggacgtt ggctttcctc
    12181 tttttggttt agatttatta aaataaaaaa aggtaatgta acatttaaat cccatgacac
    12241 taactaatat ttttgatttt ttgctttcag ctttttatat tgttttggtc tcactaggga
    12301 tccttttatt tttattttta ctaggctttg cttttatttt gtggtaccgc tggcgggacc
    12361 gcgaaaaaaa atctttggaa ttaatcaccc tccttgtcgc tgtccctcag gacaatgaag
    12421 tcaagattga tgccatggag ccagttatcg cagccgttag tagtttttac aaatcagctc
    12481 gttttaaatt tttacaaatt tttgtggctc agcccagtat ttctttagaa attatcggta
    12541 ccaatcagga tattcgtttt tatatctctc tgccgagcaa atatcgagat ttaattgaaa
    12601 aacaaattta tagcatttat gctggcgccg atatccaggc cgttgacgaa gctaatattt
    12661 tttccgaaaa tggcaaggtt gaatctgctt ggcttggtct aaaaaaagta gcctattatc
    12721 ccttaaaaac atataaggaa attcctaccg acactctttc tgcttttact tcagttcttt
    12781 caaagcttag tcctgaggag acggtcgcaa ttcaattaat cttgtctccg gtagattctg
    12841 cttgggccaa atcaggtaag tcttttatta gcggtaccaa aaaatcagag tctaatccag
    12901 aaaaagcctc ttacaaagtc gatgctcggc agcttgaagc cattgaaaac aaatgcaaca
    12961 aacctggatt tgaagtagtc attagaatcg ttgccgtagc tccgacaact gaagtagcta
    13021 aggtaaattt atccaatgtt aaggcctgtt ttggtcagct ggaatcaccc tggaataaat
    13081 tatctagtcg aaaaatatat tttaaatccc agtttatcga tgattttgtt tataaatatc
    13141 cagttgttta ttggcggaaa aacaaaacca ttctttccac cgacgaaatt gtttctattt
    13201 ttcatttacc caataaatca gtcgaaaccc caaatattta ctggttaaag tccaaaaaag
    13261 cgcctatccc ccccgatgct ccccaggagg ggatgtatat cggtgataat ttatatcggg
    13321 gcgttactca aaaattttgt attactccga ctgatagaca gcgtcatttt tacattgttg
    13381 gtcaaactgg tgtaggaaaa tcttggctcc ttgccgatat ggcccttcag gatattaaag
    13441 ccggtcgggg tgtttgtttt atcgatcctc atgatactta cgaaagcatt ttggaaagaa
    13501 ttcccccgga aagaattgaa gacgtcatct attttgatcc ctctcaaacc gaacgcccta
    13561 tgggttttaa tgtcatggag tgtgatcgag aggatcagaa ggactttgtg acttcttcca
    13621 ttattaatct aatgtataag ctttacgatc cttataaaac cggtattgtt ggtcctcgtt
    13681 tcgaacatgc catcagaaat ataatgctca cggtcatgac tgagcccgga gctactttta
    13741 tcgaaatagt tcgctgtcta actgatccct catacgttca gtatttattg ccgaaagtta
    13801 atgatcccat ggtcaaaaga tactggactg atcaaattgc caatactaat gaatttcata
    13861 aatccgaagt cctagattat attgtttcca aatttggccg ttttatcacc aatactatga
    13921 tgcgcaacat cattggccaa agtgtctctt cttttaattt tcgcgaagct atggataatg
    13981 gcaagatact aattattaac ctcgccaaag gttcaattgg tgaagaaaat tccgcctttt
    14041 taggtttagt tctaatcccc aaaattttga ttgccgccat gagtcgccag aatgtacccg
    14101 aagaaaaaag gcgcgatttt tatttatatg ttgatgagtt tcaaaatttt gccacccaag
    14161 attttgctgt cattttgtct gaggctcgta aataccattt gaatttaacc gtggccaatc
    14221 aatttatttc tcaaatggac gatgaggtca agaatgcagt tttcggtaat gtcggttcaa
    14281 tttgctcctt tcgggtgggt atttctgatg ctaacttttt agtaaatcag tttaagcccg
    14341 aatttaatga acatgatttg ttgaatttag gaactggtga aatttatcta aaaaccatga
    14401 ttgaaggagt acccaaatca cctttttcga taaaagtcgc tgcggctgaa aagaaaaagc
    14461 ctggcaatcc caaaatgtcg gaaatgatta agaaattatc tagcctaaag tatggccgtt
    14521 ccaaagctat tgttgaagcc gaaattgcca agcgcgcccg tctttaaagt tcttttaact
    14581 tgattcctct tgattcttct ttagtatcaa tcagctccaa atatttttca gtcgtcgaaa
    14641 ttcttttatg tccaacaact tgggaaacga catctagggg gactccagcc ttaagctgaa
    14701 aaacaataaa agtgtttcgg agatcattta ctttaattcc cttaatatcg gctttattaa
    14761 aatatctatt taataagctt ctgatgtttc tggcaagtaa aactctccca gttttagtaa
    14821 taaagacatt tttattttta ctattattat ctctgacctc gtctaaatat ctttttaaag
    14881 cacttttagc cgaattgttt aggggcactt ttctttgtga gtgactttca tagctttcaa
    14941 taataacttc attctcttta atattctcta tttttagact ttcaatttct ttaattcgga
    15001 gtcctgcttg aagcattaat tccacaatgg ctgtcgctct aatatcattt cgacaagcat
    15061 ctcgaagcga gcggtattca atcggtttta aaattttagg aatatcgttt tcatattttg
    15121 gatgttttac ttcggttgag ggatcgagac taatcttgcc ttcgtctttt agaaaagaaa
    15181 aaaagttttt gattgaattc aactttcggg acactgattt ggcggtatag ccattttcgg
    15241 ttaaatcttt tttgaaatct tcaatttctt tgggtccaat cgattgaacc tcggtaattt
    15301 gttttttagc caaaaaaatt atcagctgat taatatcgcc cttgtaggct acaatagtat
    15361 taatcgactt gcccttttta tttagggcat cggtaaacaa gtttaaagca tcttttaagg
    15421 ctaaattaga cataatttta tgataagtat gtcttatagt accacatatt aggccattca
    15481 tcaagctatg tttaaaaaat atctatatcg ttggattatt gtcattatta ttttttcttc
    15541 cgccctcggt atctatcagc agctaagcct tctcttaaat gctcaaaaac taatccaaaa
    15601 tcttaatcaa aaagttgtca atttagaaaa aagaaatcta gaattaaaaa aatccttaaa
    15661 tccttaaata tttactcatc tagttcattg tgctaaaatc caacagttct tttaacaaca
    15721 tgcggggtag tgtacggctg cacgtgaggc tcataatctc acaggccggg ttcaactccc
    15781 ggccccgcaa ctaacaaaga ggtcgtcatt aacttgacgg cttttttgtt tcttaaaaca
    15841 ggcattattg gtataatgct ctagcttatg gcaggcttat cttgccgtag ctttagcgaa
    15901 ggcagggtag ctcagttggt tagagcacag cattcataac gctgaggtcg agggttcgaa
    15961 cccctctcct gctaccataa aacgatcttc aatctacaat cttcaatccc aacgactgaa
    16021 gtttgaaaat taattactga agattaatta tgggcatgta gctcagttgg ttagagcgcc
    16081 gatcttataa gtcggaagtc catggtccga gtccatgcat gcctacctaa aatcaatctc
    16141 tttaactttt ttgccttcaa tgcacaataa tattcctggt tttgggtaaa aaggcaattc
    16201 cttttttttc attctgcctt ttatcaagag gttttttatg gttgctattc ctgcgccgtg
    16261 ggtacaaatt actaccgtct gatatttatt tttttctacc tcttccaaaa aatcctttat
    16321 tctcttttta aattgtaaaa aagtttcatt tccaaattca tttaatctag agtctttttt
    16381 aaaattcatt cctgttattt tcgccacgat tttagttgtt tgttgacacc ttaaatattc
    16441 agaagaagta gccagatcag gagtaatgtt ttttaaatac tctgccattt ttttaatggc
    16501 cggaatgctg tcttttagta tttttatctt ccggttatct ttggggtaag gaaggtgata
    16561 ctttgaattt tgtgtctgtc catggcgaaa aatataatat tttgtcattt aaatccccat
    16621 ggccattaat acttcgttta gagttttttg agcttcgatt cgggcttttt tactaccctc
    16681 ttcaattatt tgctggatta gctcatcatt ttttttaaaa actgcccttt tttccctaat
    16741 gggtgtcaaa aatttattta ttgctaaagc cagtttttct ttaacttcta catcaccaac
    16801 ctggcctttg atatatctat ccttaagatc ggccacctcc tgtttattct cattaaaggc
    16861 atcatgataa ataaacactg gattaccctc cacatgtccc ggatcagttg gtctaattct
    16921 gctggggtca gtgtacatgt gcattacttt ttctttgacc gtgttttcat tatctgacaa
    16981 ataaatcccg tttcctacac ttttactcat ttttgcctga ccatcggtgc caactagtgt
    17041 tcccacttcg ccaattagag cttcaggaat cggaaaaact tcccgaaaat ttttattaaa
    17101 atctctggca atttctcggc atacttctac atgactttgt tggtctttac ccactgggac
    17161 taaattagct ttgaccatta atatatcggc tgcttgcagt acgggataat ttaatagacc
    17221 cagcgatggt tgctcaatat gtaaatcatg catcacatct tttagagttg gtactcttag
    17281 ggctcttggg actgtgacta aattactaaa aattgtcgat aaataagtca cttcggacac
    17341 cttagattgt tggtaaatag tgcatttatt aggatcaatt cccaccgaaa gatagtctaa
    17401 aactaatgct tttacccgat cgttaattgt cgccgttttt tccatattca tggctgttgt
    17461 caaagtatga agatcagcca taatgaaaaa acactcatac tcatcctgta gagccactct
    17521 attttttaag gtgcccataa agtgccccaa atgtaatggc ccc
//
LOCUS       DHGB01000033            3262 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_99110, whole genome shotgun sequence.
ACCESSION   DHGB01000033 DHGB01000000
VERSION     DHGB01000033.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_99110"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT          944 a          869 c          384 g         1065 t
ORIGIN      
        1 aaatatcccc aaaattacta ttttagattt tctttttttc ataccagtat cccccaatta
       61 ctaaatacca gccccagagc atccaccagt taaattgaaa atttaccact ccccactccc
      121 attttcctac cacctccacc acctctatta aatatctcag cattggatag ctggcactca
      181 aaattatttt cccaaaacca ttccagacta aacccattac agaccctaaa aatccaatta
      241 tggttataat ttctaccaaa aacaacactc ctaaattaat aattggtgtc aacacagaaa
      301 ctgtaccaaa aaatattgaa agtatcggca aaataaataa actcacccaa atagtacttt
      361 ttaatacccc ttcttcttga ctcaaaagga ctcctaaaaa agctgtcact gacaaccaaa
      421 aactaatttc tgtaaatatt ttaaaatccg ccaaaaacat tactaaaatc accaaaaaca
      481 aagctcgact aaccttaaat tgccgcccca aaaattgggc ccaataataa atcgccaaaa
      541 acaacaccgc tctaactacc ggtatctgcc accccaccaa attaacataa agcagtacta
      601 ttcccccacc cccgataatt gccatttttc tcccgattag tttggctaaa ctttcaataa
      661 ttctccgacc gattatcatt aaattggccc cgctaaccac gacgatatgg acgaggcccg
      721 tattctttaa gaggttatat aaatctcggg aaatcagtcc cttttctccc cagaccatac
      781 cggcaagcag tcctgcctcg cccgaaggca aagccctttg aattacccct atcaaatccg
      841 acctttttat aaacttatat atcaaaaacc agattatcaa aagacaaact atctctttaa
      901 attttaattt tatctttaat tttttcataa gttttgaccc caattcctga cacttccatt
      961 atttcctcct tactcttaaa agctccccgc ctattacgat aatcaataat cttttgcgcc
     1021 gtcaccggac caatggccgg cagtacttcc agctctgtcg tcgaggccaa attaatatcc
     1081 actaccacct ccagtgcttt ttctcttttt tctactttct cgaatctagt ttccttggta
     1141 ctatcttccg tttccttcat ttttattccc aggcctgtca ccatcaaagc taaacctaaa
     1201 acaatcatta aatccttaaa tgtaattttt tttacatagt caaagttcac atcaaaaata
     1261 taacatgtat aatttaatca atgtcaactg cctcaaaaat tctttccttc ttcctgcctc
     1321 tgttgctcgc ttttttgatt catcagtcag aaagcctgac tcctttttta ccccaaatta
     1381 ttgcccttac ctccttgatc attgttctgt ttctgatttt tgcccagcgt ttttttatcc
     1441 ctctcgttat ttttattatt aatttaattg ttctttctac cggtggagtt agctcctttc
     1501 tcttttttct aatttatttt cttcttttct ctctctcttt tcaaagtcag ccagctctca
     1561 gccttatcta ctccctaatc accatcattt tttatagtac tagtctcagt tcaaccattt
     1621 cttttatcca gcttttttct ctcctcctca ttactccctt aacctatttt atctctcacc
     1681 agcaggaaac ttcacaaaaa accgaatcgg ttctaactca ggatgaaact gattttcttc
     1741 tctggatttc tcttcaccta aagtcttcac ttcgggaaat tatttccctt tccgacaatc
     1801 ccaaaatcga caaaattgcc aaagatctgc tcaaaaattc agctaaactt ggtcgctcta
     1861 tcgaccaaaa ctccgatgaa atttaaatct gttactattc tggttctact attattcttc
     1921 tcctctactc atctaatcca ctcccaacac tttacctctg attcctacat tattgattgg
     1981 ggaaatttca atataacttc cggtcgaaaa acttctgtta attaccggct caccgacacc
     2041 gttggtcaaa acgcgcccgg tctttccgaa ggagataatc tcagaatcaa agccggtttc
     2101 caatatattt atgacacctt cacacagctc tctttttcta ttgacaaact caacattgat
     2161 tttggtactc ttgtcccggg cattggcgtt acggatacca atatattaac cgtcaccacc
     2221 ccttccggcc acggttatca gataatggcg caggaaaatc atcccctctg gattaactcc
     2281 agtaacttta ttcctaacac tagctgtgac tcaggtaact gtacttccac cttctccgct
     2341 ccttggatta gttccgacaa atacggtttt ggttataatg tcaccggtat cggggcgact
     2401 gcttattttc ccgattccac ttattttcgc ccctttgccg atttttctct caatcaagac
     2461 tcaaccatta ttgcttctga aaattcaccc gttaaggacc gtcagaccat tgtcacttac
     2521 cgagttctgg tttccccttt tcaagctgcc ggcaactacg aaaattacat cacttatacc
     2581 ttagttccaa gatattaaaa tatggccgca tttaatttaa gtttagatcc agctcaaatt
     2641 gaaattaatc ttaaacctaa tgctgttttt actcaagcct acaaccttac taataattcc
     2701 accgacactc tccttctttc ttcctccgtc tctgcctggc tcccttcaga taatcaaggt
     2761 ggtgtcgttt ataccgatag tattgactcg cctctaattt tttctctctc caataccgac
     2821 ctcaagctag gcgaagattt tatcctcagg gccggtcaaa aaaaacaact tgttttaaaa
     2881 ataattaacc cctcagttga aaaaaaagat tattatttaa cctttttcat cagtcaaaaa
     2941 ccttttactc aagttcaaaa tggccatcaa aatttagcca aaatcggctc tcatctcctg
     3001 atcactaccg gccagcagtc cagttctctt tctaatttcc atgtttcaaa attaaatatt
     3061 agcccaatag ttaaggatat ttttatcccc ttaacaatta gcggtgaaat caccaatgag
     3121 ggccagcatt acagccatat caatggcaaa ctaactattt ataaaaacaa agaaattatt
     3181 tccgagcaag ctctttttcc ctatacggtt attcccacta attcccgtct cctccactgc
     3241 gtaaattcag ccagcgaggc cg
//
LOCUS       DHGB01000034           12675 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_52875, whole genome shotgun sequence.
ACCESSION   DHGB01000034 DHGB01000000
VERSION     DHGB01000034.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 12675)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12675)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12675
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_52875"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    10558..10772
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4247 a         1662 c         2823 g         3728 t
ORIGIN      
        1 aagaaagtca gagagattgc ctcgggcctg attgcgggca aaggtttcct cattactcat
       61 atagttaccg atgccggtag tggggttttg aagattattt tttaggaaat aaaattgggg
      121 taaaagccaa aaagagttaa ccgcaaaaat taataataaa atttttgcgc aatttacaat
      181 tttcaattta caattttcaa cagatgacaa aaaccaagaa ataaatatta aaaatatgca
      241 aattagataa acaataaaaa tagtttggac gtagaatgag ggaatggcga gaatgttgat
      301 gaaaattaat ttttttaatt tttggaggga caggacattg tcctgtccgt acttgatgga
      361 gtttaaatat tcccagagaa aataaatcag ccaaggaaaa aagccccaga aggtagaaaa
      421 gggttcaaaa gccacccaaa aatattggat ggagccaaaa tttagaagat aaaataatgc
      481 agaaataaaa gaaataagag gagagaattt attttttttg agaagatgaa agatgccaaa
      541 agtgcctaaa aataacatgg caaaatgcca aagatagcgg ataaaactcg taggaagaaa
      601 taaggtaaag ggcagaatta ttagttgacg aattaggtcg gtggcgtgag ccataccacc
      661 aactagacct agtccctgat attgctgcca gacagcaaaa agagaacgct ttaaatttag
      721 ccagatgttt aattccggca tgagattgtc ccagccagtg agaaaggtgc cagggatgta
      781 gttggctaga caaaggagac agagaattat gactaaaaac agggccatta attaattata
      841 cttggcttcg gacaaaagtg ccgatttgag atgcagattg atttttgcta attcccctaa
      901 cgaaatacca aagggaatag atgagtgagg aggggagcag agtaataaca acaatttgag
      961 ctagattaat aagtatggcc tttggtgaag gttgaaagcc ggttttaata gcagtgagac
     1021 gagactggag agttaaataa aaagtttttt tgggattaat tcgagaaaga gaattgtcaa
     1081 cttggcggta gaaatataaa ttgtctttaa gattggcaaa gagaccgtac tcagacaatc
     1141 taaagtataa atcaacctct tcggcactgg ttttagaagc agaataccaa gtaaagtttt
     1201 taggtaaaag agagcgattg atcatcatgt agccttgctg gacggggaca gcccagaaaa
     1261 gcatgtcatt aatactggcg gaagaaattg gaaaattttt aaaaccgatg acctgatcct
     1321 tttgattaat gatggtacat tgagcaccga caatgacagt attggggttt gattttaaaa
     1381 atttatattg tttttcgaga cggtcggagg tagaaatatc gtcggcgtcc atacgagcta
     1441 aatatttgca tcgagccata gaaatggcga tatttgaatt aatagaaaca cccaaattta
     1501 gacgattgcg gtaaatacgg atccggggat cgcgacgggc gtagaatttg attattttcc
     1561 aggaattatc agttgagtga tcatcgataa tgataaattc aaaattagct aaagtttgat
     1621 tgaggataga ttcgatggac tcggacaaat agggggcggc attaaatacc ggcatatata
     1681 ctgaaagaat aggtttttta attttgtagt tggtcattta tccaaataca gggacctaat
     1741 aaatagatcc ctgtattttt ttaaactaaa aataattatt tggtactttt ttccggagag
     1801 ggcattattt cagtaccgtt actaataatg atatctttgg gaatgctaga taacaaatcc
     1861 ggggtaactc gagctagaac taaaggatta tcgatatcaa aatttccatt tctgatttgg
     1921 tcgattattc tttgaagatc tttatcggca aaatcttttt gactatcagg cctaatggcg
     1981 atttcaccat ttctcatttg aaagtcgtag actccgggac ttaaagttaa taagactccg
     2041 tcttcaattt gataatcacc ttgcttgaat ccaccagaaa ataatctagc ggtagaacct
     2101 tcgggaactg aaacctgaac tgtttgccag gtttgttcga cggtttccag ttgctcttta
     2161 gttaattctt gattccaagg aatttcagga aaaaggaaag atctggaggt ataagttgat
     2221 cgaattccgt actcaggagt atctaagtgt tcggcaaaag aagcagtagc ttcatttaat
     2281 ccggctttaa tatcggggga actggcaatt aaaaactccg gagctttttg ggtctctgtg
     2341 acccgaggag taatttctac tggagttgaa gtactgtatc cttgtaaacc agtatcagca
     2401 tcgggagtaa aagtagctgt aataggagta ggagctttgt caacaactgg tgactcagag
     2461 ggtttacagg cagctaactg agagccaaca ataaacgcag taaatattct tacagaattt
     2521 tttactaatt tttctttatt cattttttta aattaaataa ttaatattta aacgttaaat
     2581 gttataagta tatcagatta tgacttaccc gtctataata tattttttta tattattaaa
     2641 ttttattaaa accaaaggag ataaggggtg gagtcggagc tccgccagag gcggacaggc
     2701 acccactcct tattctcctc tggccttaat ggccagaatt tatggttaaa aaaattaaaa
     2761 gttgactata acaatcaaca attaattgta aggaagtgta tcaagaaaat gcaataatgt
     2821 caagttatag gatagataaa tgataaattt agggagtaaa ttttatggac tggaaaagga
     2881 ctggaatagg ggtataataa gtttatggaa acacaaatga cacaaataaa aataaacctg
     2941 ccaatggctt taaaaaagaa aattaaagct agggcgaata agtggggact cagtttggcg
     3001 acttatatca gatatatgat tgttaacaaa gtagaggaag agtatcccac ttttgaagca
     3061 tcggaaagag tgaaaaaaac tattagagat attgaaaacg ggaaagaaaa aacaattaaa
     3121 gtgaaaaatc ttacagaatt ttttgataaa atgtaagagt ggagtatgaa ttttccgaaa
     3181 aagtttataa aaaattaaaa aggttaaaga gaaaagacta cgagttattt tccctgataa
     3241 aaattaaatt agagattttt agaatcgatg aaaaaagacc ttcgttgaga ttgcataaat
     3301 tgagtggtgg aatgatggaa agctggagta tttcaataaa tgaaagtatt agaatggtat
     3361 tttattatcg gaataagagg agagtagtat ttactaattt tggaaaacac gaagaagtgt
     3421 ataagagata gatatattac aaaatagctt agagatgttt gataaattct cttatttctg
     3481 gagcgaattc ttcatttagc atagctacag ccagattggc tttgagccag cgaagaggat
     3541 ctccggtggt catccaatca gattcagtat cggttgcttc agtaataaca atgtcattgg
     3601 ctgcgagagt gttgacggca tcggcaaacc agagttcatt gcctttaccg gtggattgag
     3661 aagttaaaat tggccataat tttttggagt taacgatgaa acggccacct tggccaaaga
     3721 aggagggggc cttgtcagct ggtaattttt catacattcc agaaatacga tttgggtatt
     3781 ttgagtcttt aatatatttg gctgaaccat aacgggcaat ttcaatgggg ccaacatttt
     3841 taacagaatc aacgatagct ggagaatatt tttcaaaggt ggagatcatt tttttgaggt
     3901 aacgtccggt ctctttttcg acgattaaat catcaccaaa catatagaca aaaggatcat
     3961 caccgataaa ggatttggcg gccaataggg gggaggcatt gccataggga aggctgggag
     4021 attgaggtac tagtttaaga gtctttagat tttggttgat ttttttgagt gaatcgaggg
     4081 cttctaattt attgacggat ttaagataag tttcccagtc ctcattggct ttaaaatagt
     4141 ttctaatcgt tttttcgccg tgacgatgga cgatgcaaag ctcagtaata ccggcagcaa
     4201 tagcctcttc gactaagtat tgaatatttg gtttactgag aatggggacg agttctttgg
     4261 cataagcttt gacggtgggt aaaaaacggg tgccgcgacc agcggcggca ataactgctt
     4321 tggtgacggg gatacttttt gtcatataaa atgattgtac cagaaataga ttagacaaaa
     4381 tcgccgtgat aatacttgtc tagagtctct ttagtgtgat agtcgatttc tcccgttttt
     4441 tccattttgg tagcttcgtc gtgggatata aatttggttt tctcaacttc gtcggtatca
     4501 ataactattt tggtattttt gggaaacttg cccataaaga ggtagccaaa agaaaggtgg
     4561 tcgcctgaat cgtattttct tttttcgaca aattttaatt ctgtgtcaaa gcctagttct
     4621 tcttttaact ccatgtgggc ggcctccttg ggagttttcc caacggggac atggccaaca
     4681 gcagtagtgg tccaagaacc cgggaaaaat ttctttttaa aactcctctg ttgaatcaaa
     4741 atctcgtctc ggtcgttgta aattaaaacg ccaatttcac gatgaagaat tcttttacca
     4801 gcatgaatag tttctttggg tagttgaccg attatttcgt ctttttcgtt aacaacgtct
     4861 aatatttcta tttgattgtc agcctggtga ttagtggaca tttatattat ggattaataa
     4921 tgtggaccca aggggactcg aacccctgac ctttcccatg ccatgggaac gcgctaccaa
     4981 ctacgctatg ggcccaataa aacagtaaat tttatcatat tgtgcaggcc tgccatgagt
     5041 cgaacatgga aaaaaggttt tggagacctt cgtgatacca tttcaccaca ggcctaagat
     5101 tgaataagga tattttaaca tctggagagt tattaaaaaa tttagagtag ggagaggatc
     5161 tttttttcga tggattcggt tttctttttg gtaacaaagg caatttcagc agaaagatga
     5221 tggataattt tgttggtaat atcctggtaa tttgcagaaa atttttcagc ggtggcaatt
     5281 ttattggcac agagctgttt taagacatag acagttttga gaggatcatt aagatacaaa
     5341 atttctttga tagttttaaa atcaaaaatg gtattgctgt caagtggttt tttgagaagc
     5401 ttataaattt cattgacttc tttgggatca attaggtggc ggagaccact tttggaaata
     5461 ttttcgacgg gaatagaaaa ggtggcgaat ttttcggttg attcaatggg gcggtaaaga
     5521 aaatattcgc attgattacc ccgataatct ttttttattt ctttaccaaa aatttgataa
     5581 atattttcaa aatcggtgag ataatctttg acagaaaatc tgtttttgag gatgggagtt
     5641 gaggaaatta tagtttatta tagcaaaata aagctaaaaa tgggaggaga tgatatattt
     5701 aagtgtgaaa atagatagaa ttgttttagg aattacgggg acaaacggag caggtaaagg
     5761 gactattgtt gattttttag ttaaggaaga agcctttgaa cattttagtg ccagtggact
     5821 aatcactgag gaaataataa agagaaagat gccggttaat agagagatga tgattatggt
     5881 ggcaaatgat ttaaggacaa aatttgggtc aggttttatt gtagaggaac tactcagacg
     5941 agctcaaaag tgtcaaaaga agacagtaat agagagtatt agaaccatag gagaagtgaa
     6001 aaaattaaaa aatattggtg gcatacttct ggcgattgat gcagatcaaa aacttaggta
     6061 tcaaagaatt cttctgaggg gaagtagcaa agatatggtt acctttgaag aatttgcgga
     6121 tcaggaaaaa agagaaaagg agtctgacga tccaaataaa caaaatttac tcgcctgtaa
     6181 aaaggcggcg gattatttaa taaagaataa tgggagtatg gaagaattgc atagagaaat
     6241 tgaaaaatta ttagttaaaa taaagaataa tgagtaatga taagtatgtt agaccgagtt
     6301 gggatgaata ttttttagat ttagccgatg cggtttcaaa gagagcaact tgtgatcggg
     6361 ggaggagtgg ctgtgtaatt gttcgggata gaagaatatt ggtcacggga tatgtgggaa
     6421 gtccaactgg ttttcctcat tgtgatgaag taggacatga aataaaaagg acggttgaag
     6481 aagacgggag tgtatcagag cactgcgtga ggacggtaca tgcggagcaa aatgcgattt
     6541 gtcaggcggc aaagctggga atttcaattg atggagcgac tgtttactgt cggatgactc
     6601 cttgtcgaac ctgtgccatg cttctcatta actgtggtgt taaaagagtt tactgtgaaa
     6661 aaaaatatca gagcggagaa gaatcagaaa aaatgtttaa aaaagtggga attgaaatta
     6721 gctataaata taatgaagtt caaaaatata ccaaaaaatg atagaggcaa tcttttttga
     6781 tcaagataat actattgtca atacaaaagc ggtagctccg agggcttatc gacgagccat
     6841 agaatttttg gcccagaaaa tggaagtaaa agtcagtaaa ctctggagag agtggagagg
     6901 aatagtcaga gaaaataaaa atagccttga tccaaaaatc agaagcttaa attattcatt
     6961 taataaactt tgcaaaaata aaaaatggat aaaagaagcc attgttggaa tagaaaaaat
     7021 gcttcacaaa gagcttgagt taaacccagg gacaaaagag ttttttaaaa ttccaaaagg
     7081 ggaaataaaa tatattttga cgacggaaga ctatcctcat tttatgaaaa taaagataga
     7141 taaatttaaa ctaaaaaatt atttcgattt aattgttgga aacagagata taggaagtat
     7201 gaaaccgagt ttaaaatatt atgaaatcgc ttgggaaaaa tttaagctaa atccggctaa
     7261 atgtatttat attggggata aatatgaaaa agattgcgaa ataggggcca agatgggagg
     7321 agttacggtg atttttggaa ataaagacaa aagggcggat tttcagatta caaatttttt
     7381 agaattaagc cagattatta aaaagattga tatataagtt taactattga tatattagtt
     7441 accaccacca ccgcggtttt gggtcctaaa agcagtggaa gtgcgggtgt tgaatttagg
     7501 aaaagtgggc ttttgagtaa agctggggtt tttaaaagga agacgctgat caaagggctt
     7561 cgtgctttga ggggtgagaa gagtgttttt tttatcattt gccatattaa taatataata
     7621 gcaaatttat agcagtttta aaaaacgatc tatgccgagg ccgattccgg aaactaaacc
     7681 taatttagaa gaaattttcg caaaattggt gtcgatgggg tgggtcggta gcttattttt
     7741 taatcgaaat tctttttcat tgttaaaaga tttcaaaata gatttagaat tagtgttttc
     7801 agtacagcca ttggcaattt caagaccgtt gatgtaaagc tcaaagcgct tggcaattat
     7861 ttgattcgaa aacgaatttt tacggtgctc gctaccttcg ggcttggctt cgccggcgcg
     7921 ttcgctgcgc tccgtaaaat tgttttcttc acctaactta gaagataagg gggtaagaaa
     7981 agcggggtag ttggtgataa aaaccccgcc gtggcggggt aatttaggtt caattttatt
     8041 gagaaaatac tgattgaagt ctggttcatt attgggaagg ttttgaggta aaacaaaatt
     8101 ggtaaattct atttttagaa attgattaat aaattttttg gttgaaagca ttaaatcttg
     8161 gtaacttttg ccaacttcgt accattcgag cattaaaaat tcgggagtgt gattgggtcc
     8221 ttcatcttct aaatcacgaa aacagtgaga aatagaaaaa caattttgat ggttattggc
     8281 taagaatttt tttagagcaa attcgggaga ggggggcaga taataatttt ggtgacgatg
     8341 agtccaaatg gttttgaagg cataaatatt ggcttcaagt ggaagggaag agttgagaca
     8401 gggtacttca atttcaatga aattttggtc ccagaaatac ttcctaatat cttttaataa
     8461 atcagactgt gtcatatata taattatata atactggtaa acaaattttt gcggtgctca
     8521 cgtcctcgta caaacgccac tcggatgatt gcgctccgaa aaattgttta cctcgttttt
     8581 aaaattacaa aaataatccg ggtcgggaca gagagggttt tttagataaa tagtggaata
     8641 gtttaagagt attttggaaa gaggatcgga aagagaacct attgctttat tggattcaca
     8701 gcaaggataa aatttttcat ctgattgaag tgagagcata gcacagccaa aattttttgg
     8761 gggataaaca ggataatttt gacgaatatt tttgacctga cgggatgtta gtgaaccgag
     8821 ccggtcagtt aaataagtaa aatttatttc tttagaacta tggagcttta gagctttaga
     8881 aaggaatttt gggaatgaat ctctatatag ataagaatct ttagtaatta aataaaagat
     8941 ttcaacggga aagcccagtt ttagggcgtg cttgacaaaa tttttagatt tggcgaaatt
     9001 tccccgacct cgattttgat cgtgaatttt ttgaggtccg tcaaaagaga cgattaattg
     9061 acagtttttt gggtctttaa ttttgccgag tttatcaatg gtgccgttgg tgataatggt
     9121 gataaaaata ttttgggaaa ttaaattgtt cacgagacgg ggaaaatttt tgagagtaaa
     9181 aacttcaccg ccacaaaaaa taatagattt aagttttatt tcgcaattct caacataatt
     9241 ttttttctcc ggcgagtctg ttaccaggct caatgtcgaa aaaaaatcat gttttcgaat
     9301 tgtttttaaa taattattgt agcgtttaat aaaacttaaa atttgcttat tagacagaat
     9361 gtttttcgtt tttttggtat aacaatattt gcagttgaga ttgcataact ctaaaggtga
     9421 aatataaata ttttccaaag aaaatataat tttttttgaa agattcattg aagattgaag
     9481 tctgaaaatt gaagatttat ttcaacttgg tcgattcttt gtgttcggta ttctttttgc
     9541 accaagggca gtatttattg aatttaagct tgtcaggagt attcgtttta tttttttcag
     9601 ttaaataatt ttgggcaccg cagactgagc agcagagggc gattaaaata cgaggagttt
     9661 ttttagattt ggccatggag ctattttaat tgatgtggcg ggaattagca ataaatcagt
     9721 tcgttatacc attatccttg aggcttttag ctagagtatc gcaaaataat tcagccccta
     9781 aatttgaggg gtgaagattg tcgacagaat caatatagtc attattacca ttttcaccaa
     9841 aaaggctgac agaaaaagtg tcagcgagag gcaaattatt ttttttggca aagttgatta
     9901 aattttgaag atatagttta atggtgctgg ttttttctat tttttcgagg gcagagaggt
     9961 gcatattttt tagaccactg gcaaagctaa ttgagtttgg ggcaatggtg gaagctaaga
    10021 ctattttgga gcttggagat ttttctttga taagatcggt aatttcttgg agtgattcct
    10081 ggtgcttttc aattcctttc tgggaatttc caaaattgtt gtaagcaaag gattcgatca
    10141 caattatgtc aggagagagg gaaagaagag cggggttttc ctggttgaga taggtcgttg
    10201 tttcagtaag acgttttatg actgattcaa tggtcgtact gccataacca tatttcagga
    10261 gattaaattt agtgcctgga aaatatttat ggagagaatt ttgacaaatg tcttcggaga
    10321 gagtgtcgat catggaatcg ccaactaaag ccaagctgac gacttttcct tcaccaccga
    10381 tgggtggcag gggtgaatat tcagtatttt gaccgagaat ttgaggtggg ggagagctta
    10441 tttcggtgtt gatgacaaca ggcggagaat gagagtagtt acttggattg taggcctgaa
    10501 ggagctgata atagagaggg aaaaaaagag ctggcatatt aaagagagcg gtattttnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgttcat agtaattaag gatagcactt
    10801 tattgatata atcagaatga aataggaggg cgggcaggac ggtaatgcgc tagttttgaa
    10861 aactagtgtc cgtaaggact aacaggttcg acccctgtgc cctccgcgta gtgtaaaata
    10921 tagttcgtaa atgcctggat agctcagttg gtagagcatc cccttggtaa ggggaaggtc
    10981 atggattcga attccattcc aggctcttgc tttttatttc aactcctact aaaatggtgt
    11041 gagtttatgt tggaactaaa gaatattact ctcgaaaaag agggaaaaaa gatattaaat
    11101 aaaattaatc tccgacttga ggaaaacaaa ttttatgtaa tgaccggtcc taatggtggg
    11161 ggaaaatcta gcttggcaaa ggtaattatg ggaattgaga ctccaactga aggtcaggtt
    11221 tattggaaca aggaagagat aagtcaaaaa agtgtggatg tcagatcgag gatggggatt
    11281 gggttttcct ttcaacaacc ggtgaaattt agaggtataa aaataaaaag attattggaa
    11341 ctagcttcag aaaataaaaa aataagaggc tgtggagtgt tgaggcaagt aggactttgc
    11401 ccgagagaat atctagagag agaagtggat gccagcttgt ctgggggaga gctaaagaga
    11461 attgaaatag ccacagtctt attgcaaaaa cctaaattgg cgatttttga tgaacctgag
    11521 gcgggaatag atttgtggag ttttaaaaat ttaattgaag tatttagaga tttgcaaaaa
    11581 aattatgctg ggacaatttt ggtaatttct catcaggaaa aaattattga aatagccgat
    11641 gaaattttat tggtaaaaag cggcaagatt aaaaaaatag aaaaaaataa aatagtaaat
    11701 ttagtgataa atagaaaact taattgtcgg gagggttgta aatgaataat ttagaaaaaa
    11761 atttattaaa aaaagtaaac gattcgccaa tgacgggagc gattaacata agaaaaaatg
    11821 gcaaattatg gcgaagaaaa agtactaaat atgtgaccat tggagatcga aaagatggcc
    11881 ggggaattga agttagagtt aaagataatt gtccttttgc tctagtggat ataccagttt
    11941 tgattactga gagcgggtta aaagatacag tcagaaatga tttttatatc ggtaaaaatg
    12001 ccaaggtaat aatctatgct ggttgtggaa tcaataattg cgggataaaa gagtcacttc
    12061 atagtggtca acattatttc tatataggcg aaaattcatc ggtaaaatac attgaaaaac
    12121 actatggaga aggaaagggt aggggaaaaa gagtgataaa tccggtgact gaaatatttt
    12181 tggaaaaagg agccaaaatg gaaatagaaa cgatccaaat tgaaggaatt gatgatacaa
    12241 atagagtgac taaagcagtt ttgggggagg gaactagttt aatagttgca gaaaagataa
    12301 tgaccagtgg cagtcaaaaa gcgacaactc gatttgaagt gaaactcaat gataaagatt
    12361 cgagttgccg agttacttca agggcggtgg cgacagaaaa atcggagcaa aaatttattt
    12421 caaatattgt tggaaatgag aaatgttttg cccatattgc ttgtgattcg attatcaaag
    12481 ataagggtag agtggcggca attccaaaaa taaaagctaa tcatgttgag gcacgcttgg
    12541 tgcatgaagc aagcattggt aaaattgcag gagagcagct ggtaaaactg atgtcacttg
    12601 gacttgatca gaaaaaagca gaagaggaaa taattaaagg atttttaaac tgagccctcc
    12661 gcgagaatcg aactt
//
LOCUS       DHGB01000035           22563 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_32933, whole genome shotgun sequence.
ACCESSION   DHGB01000035 DHGB01000000
VERSION     DHGB01000035.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 22563)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22563)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22563
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_32933"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         7591 a         3657 c         3941 g         7374 t
ORIGIN      
        1 aagtgatgaa aaggaaaagt tgtcggtaaa aatgggtagt tttcatgaga aagaaagaaa
       61 agaagttgaa gtcaaattgc aaacaggaca gtttttgtca gctttggatt tcttggaagt
      121 gctaggctac aactcgggca tgatttatta ctgggaaagc tgggaattta attatcacgg
      181 agttgagatt aaattatcaa aatacaccga tgaatatttt acttttgaaa ttgaaggtaa
      241 agaaaacgca gatgtagatg caatcgccaa agattttgaa ctgacttcat acattcaaga
      301 acaatacagg caagcaatta actgggaaaa tcaaaatatt cataaactct ataccagaga
      361 actaactgaa aaattattaa aaagtgaatt ttaatatgca catcatttat cttgccggta
      421 acagtttcaa taataaaatc tggattgaaa aagtaaaatc aaattttaat tctttctcga
      481 ctggcgacat tctctattac gatcactggc aaagcggaaa caaatggata aatttccaaa
      541 aagagtcaga aaagttagtc gaatttgtta aagataaaaa agattatttt gtctttgcca
      601 aatcaatcgg atcggtgtta actttgaaaa atatttttga aaaaacttta actccgaaaa
      661 agctaatcat ttgcggtttt ccctatcgtg ccggcaaaaa ggcttttgaa aaaattgatg
      721 aatgtttaag aacactatct attcccacca tgtttattca aaacgaattc gatccggttt
      781 atagttttga agaactcgaa aagacactaa aagaaaataa accggctgat tatcagctga
      841 taaaaaatgc tggcaataat acccatgatt atgaggattt tgaacaacta gttaatattg
      901 taaaaaattt tatgacaaat aaaacccaag actggcagga actttcccga gaagaagctt
      961 ttagaaaata ttctcgaaaa atcgaaaagg taatttttaa attaccaaac ggcaaagaaa
     1021 ctgattttta tattaaaaaa gaaggtccgg ccgctagtgt cttagcgctt acatcggata
     1081 accaagtaat cattgctaaa caatatcgtc ccgggcccaa gaaagtctta atggagttac
     1141 ccggcgggtt tgtcgaccca aatgaggagc cggaagtcac catgaaacgg gaacttttag
     1201 aagaaaccgg ctacgaaggt gacgtcaaat taataactac ttgtctagat gacgcatatt
     1261 cgacaatgga aagatattgt tttgtgtcga caaactgcaa aaaggtaaat gaaattgaaa
     1321 atggcgagga tgagtttact gagttggtat tagttcctct cgaagaattc aaaaaaatat
     1381 tacgtagtgg acagatgaca gacgttgaag ttgggttttt ggggttagat tatttacatc
     1441 ttctttaaga tgaatgtccc tgtattagtt atcctcatgg gattaccaag ttcaggcaaa
     1501 tcatacgttg ccacacatct aaagcacaag tatagtttta ccgttttgtc gggcgaagat
     1561 atcgctatcc agttatttgg cacagaaaaa gttttcaaag atcaatatgt tgaggcatac
     1621 aattatcttc gccaagaggc taccaaacta attttacagg gaaaatccgt cgtgatcgac
     1681 ggtactaacc taaaacattc ttttcggcag caaatttatg atgaagtaaa atgtgacaaa
     1741 acaattttaa tttatcttaa agtagacgac aaaacggctc tgaatagaat ttcaaaaaga
     1801 caaaactcct gctctccgga aacttataac aaattcaaaa gtcagataga agaaccatca
     1861 atagaagaaa atgtttttat tttaacttcc gacaatcgat tactggacaa tattgacagt
     1921 ttgattgcta aaacttgatt attcaagaca atgcgaagtg tcgttccagc attgaagttt
     1981 ccatctctcg tcattataag taaaaataca gataccggtg ttacttaatc ttaaaaaatt
     2041 ttttaatcta agagatgttt caaccgggaa acttcctcca aacaacatta aacccaccaa
     2101 tagagcaata aagtttgcat gagacactac taaaatattt tctttgcttt gtgattccaa
     2161 aaattccaaa gcctgcattc cccgtttttt caaatcttca aaattttctt catccgaata
     2221 atgccagctc ggatcaactg tgtacatttc tcctatattt ttaaatattg ttttaatatt
     2281 gtcattttct ttcgactggc caattgcttc ttttggtttt ctgacctcag caaataattc
     2341 actaatttgg acattgttat taattgcctt agctgtctga actgcccgaa gatgaggact
     2401 ggaaataatt aaatccaact ttgtattggc aaatctttta cctaactcct tggcttggtc
     2461 tagacctctt tgatttaaag gctcgtcgtt cgattgactg attttctttt ccaaagaatc
     2521 ggtggaacca tggcggacaa aacagatttt catgatatga tcttagcata tggaaaatca
     2581 tcaaattacc gttagggcaa ggttaatagt gattaaaaac ggaaaattgt tagtccagta
     2641 tcgtcaaaag caaaacttct atcattattt tggcggtcac ttagaccttg gcgaaaccat
     2701 attggatacc tgtattcggg aaactaaaga agaatgtggt gacgacgtgg ttttcgattt
     2761 taaaaagatt ttgtatattc gtgattttat tttacccagc gacaatgaac aaagtttgga
     2821 attatttatt cttggcgaca ttaataagtt cgaagaactt gaaggtttaa aggactccca
     2881 acatccggat gattctgttt ggctaacttg gttagacatc aatcacctac ccgataattt
     2941 ataccctcat ccactttccc aaaagttact ctcagactat aaatctggtt ttaaaaatac
     3001 tggtgaatat attggaagaa tggattaaca aaatgaatca agaaattttg gaaaaactaa
     3061 aagcagaata taaacaactt tttcaaaatg tttgggaatt gggtggcggt tatgggtttc
     3121 gctatcatca tggggttaga gtaatgaagt attgtcaaaa aatagcagaa ttcccaagat
     3181 tcaatggcga aaatcttaat gtcgatgctt tgtcagttgc cgctttattc cacgatatag
     3241 gaaaaataga agctgtcgat aaaaatggtc agctaatcta tggcaattat ggtggaatta
     3301 gccacgatgc tttgggagca aaaattgtac ccaaatattt agaaaaatat attaaggaca
     3361 aagagctaat tgatttaatt tgtttaatta tttcagaaca ggaaagtaag actacgccga
     3421 ctagaatcga atcaaagatt attaaagacg ccgaccgttt agatcattat ggagtactgc
     3481 acttatgctg ttcgataact tatgccaatt accaaaagaa aaatattgaa ggactaaaag
     3541 aattttgggg gggagatgaa ggtaaaaata aatatgaggc taacttttct aaattttatt
     3601 ttccagaagt gaaagccatt gctgaaaaaa ggtttaagaa cttatctagg gtaacggggt
     3661 taatgtttga tgaaaacgag ggattagatc tatgaatgaa gtcgaaatga acttcaaaat
     3721 tgttaataaa gacggtctac taaattacct tcgtacacta gttacagaga aaacccaaaa
     3781 tatcaaaatg acttatttcg gaaaacacgg cgatgaatca ttttatatcc gtattgaaga
     3841 gatatctgac aaaaatggtg ataaaaaagt attaaccgct aagggaagtt ttgtcactac
     3901 tgatggtgtt aatcagcgga aagaagtctc tattcctatt tcggaatctc ctgaaaaata
     3961 cattgaattt ttaactttag ttggcatgga actacgtgat tctaaatcta aagttcgtca
     4021 ttttttccat attgatgatt tagatatcac tattgatgaa tggaatgttg ccgaattagg
     4081 caacaggctt gaaatcgaag gtcttgaaga aggaaaagta aaagagtttg ctacgaaagt
     4141 tatccaatat tgcgatccta aaccttcaaa atgatataca atgaagcaca aaatatagat
     4201 acaataaaca atttgattgg tatcttcaag aggcggttgg caagacaatt tggtcagagt
     4261 ctagtgcttt tcgatatcac tgcttatcaa aataaaaatg aggttattct taacggcgag
     4321 gtgggtactg tcaaattaaa gaataaattt atgagcataa tcaaaagaaa aaaaataact
     4381 gtaactgata atattaaaac tttatcagac ccaaaagatc atctagaaat tggttggggt
     4441 aagtctctta tagatcaaag cacatatagg tctccagaag agcagccgaa actcgcaacg
     4501 catattttga gtggcgaaac attccgtgtc ctaaagcaaa ttagtgggtg gtacttagtt
     4561 cagctagagg acttgtctat gggatggatc cgaatattaa acaaggattg tgttgtggca
     4621 tttaacgata aaagtccaga atatggtgaa ttttcagacc gctggcagat ggttccaaga
     4681 gtaaattcta ctagattaca gttcgtcttt ccagatcagg agacactgga gaggaaatta
     4741 acagatatat gttcaccgta tatgggaatg ccgtatatat ttggtggacg ttcacctaaa
     4801 ttcggtttcg attgttccgg attgatccag aatattattt tcaaacttga gaatgttctt
     4861 ttacctaaaa acagtttaga tcaaatcgta ctaggaaaaa aagccaatat aaaagtgttc
     4921 gataaaaacc aattcaaaat tggagacata gtttttatta ggatcagaaa gaagattcct
     4981 catagtggaa tagtgaccag tcgaggcatt ttacatacag aaggtttaaa tcaaaaaata
     5041 gttcttatcg acagttttga ggatattgct aatccggcta aatttgctca tcaatggcaa
     5101 agagattttg gtaaagcagt taggtttttc cagtttcaaa gtgactagaa cctaaatggt
     5161 aactggtttt tcaatattgt atttactaat tcactgttaa ggtcattcaa tttgtttatt
     5221 ctgtatccat ctgcaccact catccattcc tgaatgacaa aaagcttttc aaagcacaaa
     5281 ggtattacca ccaaaaatag cattatctac gccaaattta attttccaaa gtggtctgaa
     5341 aaaaggagaa attgacattt ataaacaaat tatacaggtt ttcatatcaa tgatatatta
     5401 cggattgttc catagtatat tccaacccca ccttaattca attgggattt gtctgaacaa
     5461 aaaatagtat aatgctacta aattatggag gtagtaatcc gaccggtaaa accagaagac
     5521 tataaagaaa taaagaaata tctatttgtc cagtctgaag aaaaagaaat taaaaaaagt
     5581 ctgacgactg acattgagga aatgggtaga aataagctac ttagaattgt ggcggaaacg
     5641 gataataagg taatcggtca gtgtgatttt aggcgattaa tttctcctat taaacaacat
     5701 attattgaaa tcaccggttt ggtggttaat gagaattacc aaggaagggg tataagttca
     5761 atgttgataa catatggtct aaattgggct aaagaacaag gaattgagat tgccactatt
     5821 tcagtacgta agggaacaaa ggctgaggct gtatatttgc atatgggatt taaggtatat
     5881 gggaaattac aagatgggat taaagaaccg tggggagata aactaagctt tgatgaaatt
     5941 tttttgttta agaatattta actcattaat catgagtgag gctcctcaga ccagcagata
     6001 ttcctactgt acctttagat aaagaagtag tgtctcgagt aaaaaacaga ttaatatttc
     6061 agctctgtca cagagatgtc atttattggg aataaagaaa gagagtcttt aataattttt
     6121 gaagtaatga ctgtcaggct acaggtcatt aagtcgatat ggagggcatt attttctggc
     6181 caagagtgga taattaaatg tgattgagat agaatccaaa attttgttaa accattattg
     6241 ggaaactgat gtttttcttc tttaaccaca tttaatttca gatttttaat caatgaattg
     6301 gcaatttggt cagttaattt ttcgttaatt ctcacttctt tagtgaaaat taatttgaca
     6361 acatagtgtt ggaaaaatgg aaaattagtc atttgtatat attttttctt gttttaaatt
     6421 aacggttttg gtactcggtg tccagtctaa aatgtacaac gagctttgcg agccgatact
     6481 ttccgctccg taatatttgt taaattgatt gagttttgtt ttaataatta aattgtgatc
     6541 aagtatcaat ttttgaattt caatttccct ctctctggct cgatcactat ttccatagca
     6601 aagataaatt cgacctaaaa agtttttctt gattaatttt atcgcttgat ttaaaaataa
     6661 attaattccc tctttggtat aaggaggatc ggtaaaaaca agatcgtatt taccaaagta
     6721 acttttatct ataagctgac gaagatcctg tttaaccact tcaatattta agttattttc
     6781 ttgagatatt tgtgttatta gattacttaa tcggtcgtca atttcaaaaa cggttatttt
     6841 tttacattga tgagttagag ctgccccgat cgaaaccaaa tcatcgtctc ctaaaaaagc
     6901 aatttcttta ccggctaagt ctccgtttct ggctaattta gcaactcttt taaccgttgt
     6961 ggctatggtg gcattgaatt gatctaaatt gcggtctggt tttggcctat ttttttgata
     7021 tttatttatt atttctttaa tggcattttg gttaattttg atttctaata acattctttt
     7081 tgcatagttg gcaatttttt tagtcaaatt ttttttggtt ttgatgttta cacttttggg
     7141 ttctagaata tctgaaaact catgaattaa cctataaaga tgtacctgtg gtaatcctaa
     7201 tttccttgtc aaatcttggc ttgaaagatt tgaagagtga gaaattaaat ataaaaaatt
     7261 taccactact atcctacgaa gttccaattt ttgactggtt atttctattt tttcagataa
     7321 ctgttctcga gacatatcgt attataaacc gtcccattag aataattatt tgaataagtc
     7381 atgatacata ttcgacagtg gtctggttca tatttctgaa atttcctggg aaaaagttaa
     7441 tgatgtcacg gacatgttta ttttttaaaa ataactaaaa cagaataact ttggcggagt
     7501 ttgtgggtct ggaacctttg tgtcactaca actttattta gttaaaaata gacaaacggg
     7561 agactataaa aaaggtattg aaaatataat actgcataag gccataaaca tattgcactc
     7621 tttgggatca ccgtagttat ggcttttaac catccaatcc acagtctgtg caaaggaatc
     7681 aggggatttg tcttggtctc tcgtcaaggg ttaagatacc gggatatgat tactaaaatt
     7741 gccctacaca aggctaagat acttactttt tgggataagc atggtttggt agctacttta
     7801 gatgctttta aaatcaaaag aaaaagaagt tggtctccgg aagtaatcga agaaattaaa
     7861 agacttcact taacccatta ccatacttat cccagaaccc caaagatgaa tgctcattgt
     7921 gaaagattta atcgaactgt tcaagacgaa tttgttgatt accacttaga tgaaatgatt
     7981 gaacctgacg tatttaatca aaaattaaac ctaatagaaa aagtggttta acaaatgggg
     8041 tacttgacaa acagacctag aaaatgcctc gattatgaga cccctatcga agcattcaaa
     8101 agagagttaa atgttatcaa ttacaagggt attcggattc acattagaat tcacctcggc
     8161 tgtatactct ggtatttcaa aactaattcc aacgtggctt tgcgctgcaa gattataaat
     8221 ttcgtctggt ttaatcttat tcaagattgt tctgatagaa ctgacatcgg tcaaattcgc
     8281 aaagtggagg gtaacctatc ctttctgaat ttctcgctga tatggtcaag atttgaaaat
     8341 tcttcaacgc tctttcttct tactagacca tgaactatat aatctttttc taaaagcaga
     8401 tccgttaaat atgatccgtc ctgtccagaa attccagtaa taagtgcagt tttcatatag
     8461 gtatttaaat ttaacgttac tattcatcta aaattcttat aatattttcg catataaatc
     8521 gtatctggct ctcatccaag gatggactag aaggcagatt aatacctgca ctagctaaat
     8581 actctgtgat tttgaaattt tcatgacaat caaaccgctt gtatctatgg ataggataaa
     8641 agaatggacg actatctatg ccaacttctt taagtttaaa cattaaatta tcccgagaaa
     8701 ttttgcctgt tgatgttact aatatcgaat acatccaaaa cacgctatca gcccaagact
     8761 cgtgaggaga aagctctatc ttttgaacaa taggttttaa atatttctcg taagttcctg
     8821 caatccactt cttctttgca attattttat caattttttc taattgagcc agacctattg
     8881 ctgcctggat gttggtcatt cgataattaa agccaagcca aggataataa tatcgtcttc
     8941 tttttgaggt accatgatcc ctcaaagttt tcattttttc tgcaaacttt ttattgtttg
     9001 taacaaccat tccaccttca cctgtagtga ttattttgtt tccaaaaaaa gaaaaacacc
     9061 ctgcgttccc caaagagcca actctattgt ttctatattt agctccatga gcttcagcca
     9121 catcttcgta tacagccaaa ttgtactttc gcgcaattga aagaatttgg tccattttag
     9181 ctggatgacc ataaagatga acagggatta tcgcctttgt atttttgtta atttttgact
     9241 caatttgctg gggatcaata ttccatgttt gtagttccga gtctacaaat actggtttcg
     9301 ctcccgtata cataacagca ttggctgtag caacaaaagt caacgaagga actattattt
     9361 catcgcctgg accaattcct gaagatgcta gacataaatg caacgcagta gtaccacttg
     9421 aggtagctac tccgtacttc aggccgcaat aactcgcata tttgctctca aactcaagta
     9481 tgttctcgcc tatagaagaa atccagccgg acttaactgt cttaataaca gcatccaatt
     9541 cttcttctcc aatgtctggt tgagcaaccg gaataaacat tcatcctgat aaataattat
     9601 gaccgatttt agcacaatta attagaattg gtataagtcc gatttggatt gacgtaggat
     9661 acctgacatt agctcattaa tagctttttg atgattctga tattgattag gaaatatcaa
     9721 atgactttca agttcttcgg ctttaaccca cttatgagtt cgaaattctt cttttgaaaa
     9781 aactaactta cctttatctc caataaatct taatattacc tgatcatatg actgtcccct
     9841 gtatttgcct ccgttaaccc tgagcgccgt gtctgttgga taatcatatt caattttaaa
     9901 actactgatt ccaataattt caaaatcaga ttttgtagta ccaatctctt cattcaattc
     9961 tcggaataga ttctgttcaa gcatttcacc ttcctctcgt cctccaccta aaaaattcca
    10021 ctcattttct ttatatccat ttttttgaac aagaagaaaa ttgttacctt tatcaaccac
    10081 aatggcatta acacctagac ggtaaggctt gttttgaata ttatcattca tatttatatg
    10141 taaatatcag gaagttttga tttctccttt agaaatctta ccatactcct atatttattt
    10201 cctgtaggat ctatcctgtt cattttttct tttgcttttg taaaaatact atcttgaaac
    10261 tgtctatcac ctccatgaca aacagctcca taactccaat gtcttgctga aattttcatc
    10321 ctttcctcat gaaagctttt ataatctctg aacttatcta caaaatataa tttattatta
    10381 gattctttta tagacttcga caccttaagc atctcagtag ttgattcaca taaagcaatc
    10441 caaatattat tgaaatttgg tgaaacaaaa tagttaagaa aataggaatc aaagcttggg
    10501 tcagtactta aatatgcaat aaataccaac gcagttatct gtgaggcggt aaatccatcc
    10561 ttcttaatca ttccacctaa gaatccagca agaggtgcta taaattttcc ggcatgagac
    10621 aaagcttctt ctttgagaat gtggttacta tataaaaaaa tgtgaggatt gataatcttt
    10681 ccagaaaaat aacttccata cagccgactt tctccatcat cttggagaat cttcatattt
    10741 attcctgtaa agtggatggg aatactttta aaagaatcaa caattttagt atctttttcg
    10801 aaataatcat taatggcgat tagatccaag tcgcttccta cttcgtttcg attgataaca
    10861 aaacttccga atgctagcca tagaccttgt ttgtaattat caaattttag tttttccgat
    10921 atatgtttaa tcgtttcttt tataaaagct aaattttctt gggatattac tttctccata
    10981 ttagtacccc atatctttaa gaaatatttt tattccttct ttagtgtatt cgtggcttaa
    11041 tgaggactcc agaatctttt taggtacggt tatcacatca actccggctg atattgaatt
    11101 ctggaaatct tttgttgttc taatagaaga agccataact ttaactgact tacctaaaga
    11161 atcgacagac atggcagtta aggtgtctag tagtaaattt ttaccagcaa ttcggtcttc
    11221 gtcttttaca ttattaaatt gcttaatatt atcaacatat ctacctacca gtggacatac
    11281 ataagtagtt ccgacaattg ccgccaaaaa tgcttgacag gggttgaaaa taagatgggt
    11341 attaaactta atgcctttct ttgaatattt ggatactatc tttaaattat caaagtcggc
    11401 aggaatttta atcaccactt tttctgaatc aaaccatttt tgattaattg tttccaataa
    11461 gcagttttct gtttcttttg gggttggtcc taaggcttca atatgtaatt caagatttgg
    11521 aaattcagta cgaaacgaag aaatgaaagc gttgtaatcg atgttttttc ttttaaagaa
    11581 agttggtgtt gtcgttacac ccgatactaa ccaggagaat tctctgattt cgtcaataat
    11641 aactgaatca agaaaaagtt tagttttcat aattttttat tgtgttaaga aggtaaacgc
    11701 cataacaaaa agcacaccat tgttgattgg gattatttgc aagatgtggt agcaaactga
    11761 gccaaatcgt gacgtgtata aataggataa gttctggatc ttccttaaat tcttgaaata
    11821 ttctcttttc atagtttctt aatctattat cgacgattga aaaataatgc gtattaatat
    11881 ctttagtgtc ccttttataa ctaaacttac ctacattaag agaatcaaag ttcctaataa
    11941 tgctatagta aatttttgca atatcatatc gaatatctcc aaagaactga gtattcccaa
    12001 aacaccctct tggatctatt aagtaaagtt ttcccttgtc atcaattaga gtattactca
    12061 tggtaagatc gccatgaatg aatgagtatc tcgtatcttt taatggtgtt tccaaatctt
    12121 tatataattt ttcaaaaagt tctatcggac tcgtatatct attcccgtta ataaccagat
    12181 tgttaacatt tatatttaac ttatcgatga aatccactaa agcatttatt ggcttggtgt
    12241 aatacatcat ataaatatcg tttgggtccg aaactatggt attttctgca tgaagtcttt
    12301 ttaccagaag tattaatcta ttaaaagcat ccggaaagtc atttttgtgt tctattgccc
    12361 actgatatag attagttccg tttatggctt ccatttcata atatttaaga tctcgaccat
    12421 ccgaacattt catgttgaca tcataactgt agatgaaagg aatgtcgttt ggaattctct
    12481 ttgatgcttc cttaaaccaa ttcgtttctt taaagaatct gtcttcaggt actcctttta
    12541 taactctatt atctttaaat agaatctgat tataaaatct tgaaacaatt ttttgatttt
    12601 tggatttgct attagtcatt ttcttgcttt gaacatttag cacaattgat atggtcaaac
    12661 aggatatttt tattttgatt gagatcgttc aactctggca catgaactcg attgtataca
    12721 tcccaatttc ctactttgac tacatcctgc ctcagcaacc tctttatttt ttttaattca
    12781 tcaatggaat tctcgccgta tattaaataa gtaaacacac agtttgtcaa atattcctcc
    12841 attagaatcc gaagttctcc gaatctatca ctttgagcta caacatctgt actaaagata
    12901 tggggataca atcggtgaac ttggtctctt gttagaacgg tgcatgaagt tgcgataaca
    12961 tcaagattaa aagatgataa atattcttct gttttatcct gaatcgatgt tctgcctaca
    13021 gcagaaaaac atcctggttt taacattaat atacctagct gacctttatc actttcaccg
    13081 ggcaacaata ctttcagttg tgagtcagaa agattatgag gccttatgcc gatatttttt
    13141 attagtcttt ctttaggtac ctctgcagtc ataatcaatt gaatgcttta attgttttct
    13201 taatcgtttt gacatcaaga tcatttttta taaaagatct caatttctta agcacttttt
    13261 tgtcatcatc gtctactaac agaatagctt tattgagttg catccaggtt gttgatttct
    13321 tctgtttact taaaccaaga actgaagtgt catctttgat cttaaatgag taagaaatta
    13381 tattgtggtt ggtttcatca aaaacttggt gacttggaat tacaacctga tactcgctga
    13441 tttctcttgt ttttttaatg gcagcctgta accaggattc gtttattctt ttcgtggttt
    13501 ttggaacaga tagtttcttt ctactatttt ctaataatat ctctacttcg ttcttagaca
    13561 atctataagc tatgcagtaa gaagcctgtt ttatatttag aaatgattta tacaagttca
    13621 aatgatcgtt agcttgtttt tgtaagttgt atttaaattt taatgaccat aaggcatttt
    13681 ttattaactt tttacaagta gttgagtcag taagtacttc ggtaattgca tcatttaatg
    13741 atttacagtc tccatatttg cataaaatac cgtcaaagtg atttgttatc aagccgttaa
    13801 taccaactac atccgatcca atgacagggc attcacaagc catggcttct aaaaaaacag
    13861 taccataacc ctcagcctct gaacacaata cagtaatatc agcaatattg taaagtattg
    13921 gcatgtcttc gtctgtaaac catccgtgtt caacaaattc tatatctcct tttaatttct
    13981 taaatgactg atatatcaat tcctcatcct ggttttgagg taaaccagta acaattatct
    14041 taatcttgct attcggaata gatagtttag atagcgcctc tgacacgaag taaagacctt
    14101 tccttttcct cagcatgcat ggaattagta ttacaaattc ttttttggat atgttgagac
    14161 gtttccgcat actatccaaa gttttctgat tgttgttctt tttgaaaata gttctatcaa
    14221 ttcccggata tatcagacga attgcattct cctttatccc tgactgtaaa aggctatctc
    14281 tataagctat tcccggagct acgaatgtga caagtttgct tctatttaca taatcaaatg
    14341 tagaccaagt actgttctta ataatgcttt cttctcgata cattggtgtt ttagtatgtg
    14401 taacaatgtg aggaattcca taatttaaat ttgcaatttg ccctattagt gattcctcaa
    14461 tatagactgt atgaagaatg tctggtgatt cgtctcttat ttgctgcatc aaaacatcaa
    14521 ctccctgtat aaatccgcct ttatcgtaat ataaatcttc aaatacttta aatcgtttta
    14581 tcgtcaaata attatttatt ttatcaactg aatcctcaga gccactagta attaagacaa
    14641 ttttgatttt aagttttata agttgagtaa tgaggttttt tgtataactg gcaccaccac
    14701 cagttattgg tggatactcc atatgaaaat aaagcacttt aggactaatt gttttcatac
    14761 gcttacctgt taaaccggag caaatctaca tgctcctgac cgtttgaatc agtaaactta
    14821 cccacgatct ggaatccgaa tttttgagtc aaatgtatag ccctcccatt gttggcaaag
    14881 ataccgccag acagcatgat tactctcaag ccaagactat ctgacgtatc gattacttgt
    14941 tgaagagcaa ctgcccccaa acccacgttt tggtaatcat cagcaataca taagccacag
    15001 cgacttacag tacctggaat aaggtcgaca ccatattgtg taaatctcac tctatcggcc
    15061 tcgggcaagt ctaaactaaa tttcattatg gcaattattt ttgacaaatc attctctatt
    15121 acaaaatgta acttcttagg attacttata gattcacaaa gtcttactgc tgtcttctcg
    15181 ccataatcgt ccaaaacata aaaaccctga gttacctcag ataaacttgt ataaaaagag
    15241 accaaacctg tcttatccat cacctctaga ggtcttataa aagcctttct tccatcacca
    15301 atcaatattt gacttttttt tataagacag tggttttcat tttcaacaac atcggtcttt
    15361 atttcttttt tagccacttt tctgacttaa attaagtatt gcgcgtattt tacccttatt
    15421 atgtcacttg tgcaatatga tgtgcaggtt gtatatattt gagactctgg gtatcttttg
    15481 agtaaataat taatgggagt catattgtca agtacccctt tgtcagacta ttttgtgaaa
    15541 ttgctcaatc caaacagtaa catggaaaat atgacgatgg tcaaaacgca aaaatgccat
    15601 ctcatccggt gcatttggat atttgtgtat accttcaaat tgggtagtaa ttttagccca
    15661 gatagttttt ttcatattaa ttagaattga ttagcttttt tcaataacac gaagttttcc
    15721 actacgtgct ttataattag caactatctc ttccggagtg gcagttattg gacgtttagt
    15781 tatcaaaatc cctatcggct taccgttctg tccgaaagtt cgcgctatcc tattgaaagt
    15841 atgtttaact atcgtatcct caaatgaatg aaaactaatg gcggccataa aaacgagatt
    15901 tatacttcac aataaacaac tgacgattaa aacttaaata tttgacatgc atttgccatt
    15961 ctgataacct aacgataact taccgagcgg taagtaaaat aaaataagcc aaaactatgc
    16021 aagcaaccac cataacatct gaaggggtag ttcaaaatac taaaaaatgt atcattgata
    16081 ctgcccgttg gtttttctcc gaatataact acttggccgt gtcaatgagt gacattgcta
    16141 aaaaattaaa tattaccaag gcagcacttt attatcactt caccggtaaa gcagagattt
    16201 accaaaaagt gcttgacgat gtttttaacg atttaagttt aacgattgcc gaggcttcga
    16261 cagaggtaac aattgataaa aaattacatc gtttaattaa gaattactta gactttggtt
    16321 ttaaggaaaa gaacctagtt aaagcttttg tatttaaact atcatcgtct gattcccaaa
    16381 taaccaaacg tattattcag ttaagagaac aagttattgg tttaattcaa ctagtaattg
    16441 aggaagtgtc aatcagtaaa aaactaacca aaaaaattga tagcaaacta ttaacctcat
    16501 tacttaccag catgatggac ggattacttt tggaatattc atttcttaac aaaaaaatgg
    16561 attcagtaaa aatatctgat caaattatta ccctattgtt ccaataatta caaaaattat
    16621 gcttagcatt ataatcccaa cgttcaatga agaaaaattt ctaccttatc ttttaaggtc
    16681 gcttaaaaaa cagactttta aagattttga agtaatagtg gccgacaata attcgatgga
    16741 tgccacccgt ttgatcgcct tgaaatcagg agcaaaagtg gttgagggtg gaatgcctgc
    16801 tagtggacgc aataatgggg ctaaatttgc ccaaggtgaa tggttgcttt ttttagacgc
    16861 tgatgtgata ttaccgccag attttctgga aaaagcgata aaagaaataa aagaacaaga
    16921 gtttgcggtg acatcctgtt tagtaaaacc tttaagtaat aggaaaattg ataaattact
    16981 gcatgatacg gtaaattggt atatgggagc cactcaaaaa tttttccctc atgcacctgg
    17041 attttgtatt tttgttagga aagaagtcca tcgaactata gggggtttta acgaaaaaat
    17101 aaaattagcg gaggatcatg actatgttct aagagccagt aaaatcgcta attttggctt
    17161 tctccaaaat gtccggatac cagtatcggt tcgaagatta gataaagatg gtcgtttaaa
    17221 tatttccgca aaatatttgg caactgaggc gcatttagtt ttattaggac cgatatattc
    17281 aaatattttt aattataagt ttggatatcc aagcaaaaaa taattattaa taatttaaag
    17341 gaaaaattat gtcgaaaaca aatcagccga aaaattcatg ggaggagata tttattaaat
    17401 atacagataa accgttggag ttattggttt ctaataatat tgttagaaaa aacctgatca
    17461 aattggcgga taaaaaaatt cgtcaaggtc ttttagaaga taaaaattat ccccgaaaag
    17521 ttcaggaaga caaatattac atgtcgagaa gttttattac agccatgaat aaagcctttg
    17581 agggggctaa aaatgcacca atggtacgca aagcattagt caattctctg attcaaaaca
    17641 tttttttgaa atcaaaaaca caggtgaaaa aatttcataa agaatttggg aggaacccac
    17701 cttctttttt aacgattagt ccgggaaaat tctgtaattt acaatgcacc ggttgttatg
    17761 ccaatagtaa ttcggcttca tcggaaaaac ttagttatga tgttctaaac agaatcgtaa
    17821 cggaaaaaac taacctctgg gggtcttact ttacggttat ctctggcggc gaacctttgc
    17881 tttatgaaag ccagggaaaa actattattg acttggccat agaacatcag gataattttt
    17941 ttctgatgta taccaacagt actttgattg ataaaaaaat ggccaaaaaa ctggctgaag
    18001 ttgggaatat tactccagca atttcagtgg aaggttttga gaaagaaact gatgccagac
    18061 gcggtaaagg tattcacagg aaaattttag aggcaatgaa aaatctaaga gaggctggag
    18121 tacctttcgg tatttccgtt actgcaacta aaaataatgc cgaactgata ttaagtgatg
    18181 aatttgtaga ttattatttt aataaacagg gagcaattta cggctggatc tttcagttaa
    18241 tgccgattgg gagagcagat tctttggatt tgttggtgac tccggaacaa agattaacta
    18301 tgtttaggaa aaaccatcag ttgattaaag aaaaaaagat ttttatggct gatttttgga
    18361 actgtggttc agttaccaat ggttgtattt cggctggtaa agaaggtgga tatttataca
    18421 tcgagtggaa tggtaatgtg acgccttgtg ttttcaatcc ctatgcagtg gctaacatta
    18481 atgaaattta taaaaatggt ggaaatttaa acgatgtttt gaaccgacct tttcttgaaa
    18541 aaattagaag atggcaggat gactacgctc taaaacaaaa accggaggat atagataatt
    18601 ggattctacc ctgtcccatt agggatcact atgaagaaat gaagaaattt attgatcaag
    18661 ataagcctca accgattgat gaagcggcca agcaagctct aaaggataaa aaatataaag
    18721 aaggaatgat taattatgat aaaaaattag ctaaaacctt agacccagtt tgggaaaaag
    18781 aatataaaaa ttcaaaatta ggttaattta ataatttagt gtagaaaatt taataaatga
    18841 tgaatattgt tgcttgtata gccttgggag tagtgatggg tttaactgca tcgattatta
    18901 tggatatggt taaaaccaat cctcgtcccg atacagtaac gaatgtggtt ttaggtgtag
    18961 ttggagctgt tggagggggt gttattacaa gctttgttgg atggtcaggt attacagagt
    19021 ttaaaatata tggtcttgtt actgccataa tcggcacaat tattactatt ttgatgaaat
    19081 tgtttgagag ttttatcatt cataaaatga aagaagcaaa caaacgggaa atgatgataa
    19141 gagttttagg caactttgaa agattaccga tttctttaca aaaagtccta gagaaggcca
    19201 tagatttaac aaaaaataac caaaaaatga tcgttaacgt ggctttaagc tatggaggta
    19261 gagatgagat catcagaagc ttccgcaaac taacagcttt agggactaaa gtcgaagaaa
    19321 tcaccgagga attaataagt caaaatctag acacagctgg tctacccgat cctgatttca
    19381 ttatcagaac atcgggcgaa cagcgcctat caaactttct tccctggcaa gcgacttact
    19441 cagaattgta tttttctaag gtttattggc cagactttga caaaactaaa ttggatatta
    19501 ccattttaga atttcaaaat cgtcaaagga ggttaggaaa ataaatgagt aaaaaaagca
    19561 ttttttataa atatccccag ctatatatct ggggtcttag ttggattcat ggaactaatt
    19621 ttgctaaacg ttatcaatat atggccagct ttgccaaaaa gggagactta gttttggaac
    19681 cagcctgtgg accggcaata ttggcaaatt ttttaccaaa agggttcctt tatagtggtt
    19741 ttgatactaa tgaggaattt cttagttatg ctctggaaaa aggcttaaat gttcgtttag
    19801 gtaatgttct cgaatcaaat tgttattgtc cagcggatgt gattattgtc tgcgatgtcc
    19861 ttcaccattt aaagccagac gagagaaaga ggtttattaa aaactgtttt tcggcaacca
    19921 aaagagtgtt tattatttgt gaacccggaa aaaaagagga aactggtagt ggtttgatat
    19981 attccttaaa aaaacgtctg gcggaatgga gtgaaaaaga cggtactggc gattttaaag
    20041 ctgaatattt ttcaactcgt cgtcaacttc tgaatcaaat tgaatatggt tttgaggtta
    20101 ttcccccatc aattaaaagg gaggtaaggg attttggaga agatatcatt acagtgtttt
    20161 ttaaagatac aaaatgaaaa aatttgtttc agctatagtt ccggtattta atgaagaaaa
    20221 aactgtggct ggtgtcattg aagttttgtt aaaaaatcct ttaattgatg aggtaatctg
    20281 tataaatgac ggatcgactg ataaaagttt ggaaattttg aaaaagtttg gtaatagtat
    20341 taaattaatt aattttgaga aaaacagggg caaaagttac gctttggttg agggtataaa
    20401 aaaagctaaa ggcgaagtaa ttacttttat tgatgctgat ttaactaatc tctccgataa
    20461 tcatctaaaa aaccttctag ggcctgtttt agaaggtaaa tttaaggttg ttttgggata
    20521 tccatcaaat ggatggatgc aaaatgttct ttcatattta actggcgaaa gagtgtatta
    20581 caaaaatgat ttagtccctt atcttggaga aatggctaaa accaagtttg gagtggaaat
    20641 ttttttaaac aatctttttt ctaaaaagaa aactaagaag attcccttga aaaatctgaa
    20701 aggtcttctt aaatacgaga agcgtaattc aataaatgct tttagagaat atgtggttga
    20761 gggtgtagaa attgctcagg aaataggtcg aagggaaggt ttactaccaa gagattatcg
    20821 aattattggc cagatggcta aggttactaa tcttaaggag ctaaaaaata aaattaaaga
    20881 gattgcaaat aaagatgtca gacggttttt agaaaaatat gttctaaatt atataaaagt
    20941 tgtccgaggt tggtggagaa atttttacta atgattttat gctcacaggc caaactagaa
    21001 aacgataatc ggggtataaa tgtaaaaaga ggcatgagag ccaaatgtga agcaggctgg
    21061 cgaccaaata ttgctccaat tgggtatctc aatgtcatga gctataaccg aatcagttat
    21121 gtagagatcg acaaggtacg ggctccgacg gttaagcaaa tgtttcatag ggtcggagaa
    21181 aaagggcaaa gtggaagaac cattaaaatt tggttggata aaatcggatt tgtaacaaga
    21241 agtggaaaac cgttggcatt aagtaaagtt tataaaaccc ttaaaaatcc ttattattac
    21301 ggcgaatttg aattcggagg cgtgacctat aaaggtaaac atgaaccatt aataactaaa
    21361 acattatttg ataaggtcca aaaacaacta tatgttccag agaaagaatg gcataaaaaa
    21421 ctatttcctt tccgcggatt atgtaagtgc ggtagttgtg gagaatggat aacggcagaa
    21481 gaaaaataca aacgatataa atacggaaga tttaagaaat atgtctacta ccactgcgga
    21541 agacatatcg attatgactg cgaagaacca tacatcagcg aagaagaatt aataaaccag
    21601 ttggtaagcc acatcgatga aatcaaactt gatgaaaaag ttttaaccaa tcaattaaaa
    21661 gatgaaattg aaagattcca ctcgctgaga ggcgaagtac ttcatcaaga attccttgct
    21721 ggaaatctaa accaatttga ttatcctcaa aaaatacccc tcgatcgaga caccgccaaa
    21781 gaatacttac tccacgttct aaaagccgga tcggcggaag atagaatcca aataatgtcg
    21841 gcaattaaga caaaatttat tctgcataat aaagaactaa ccattaaaaa ttaaacttta
    21901 tttgttttaa tcgaatattc aacagcgttt tttttaaagt tttctatata ctcatttgct
    21961 tccgtttctt tatcaaaatt aagaatcagc ggtaatttat acaatttttg tttaagatca
    22021 tcgattgata attctggata gtagttctta agaagataaa ccaaattaaa aattttttca
    22081 tctgaaaaac ccaaaagaga tatttctata gattttttat caggttctct atgaaaaata
    22141 taatcaaata ttttttggag taaataaaaa ggaaaaatta tcgggagaag tactactaaa
    22201 gcaaaaaaat atcctgtaat tgttgcaaaa ccgtataata acaacattgg aatacctatt
    22261 attcttgggt atttattgta aaacgtacct gctcgaccgc tgtagctttg aaatgaaaag
    22321 tcatcaaata aaaaatgtaa caatcgttta aaaatatttt ttagcattat tctgtgttct
    22381 aaaggtcaaa tactccctgt attttagcat tcaagggata tatattatca ccgtgttaca
    22441 ggctacttta ccgaaaaatg ttgtttttgg agaataaaca ccaacaggtt ggggaaatac
    22501 ttgactaaca ctggggtcgc atagaacttt gaccctttgg cggaaggggg gggagtcgaa
    22561 ccc
//
LOCUS       DHGB01000036           19343 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_70745, whole genome shotgun sequence.
ACCESSION   DHGB01000036 DHGB01000000
VERSION     DHGB01000036.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 19343)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19343)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19343
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_70745"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         5876 a         3027 c         4283 g         6154 t
ORIGIN      
        1 tctaaaatct aattagactt tttttggctt tgtaggcaac accttgggct tgtttttctt
       61 tgaatttatc aattcttccc ttaatgtcga caaatttatg ttgaccagtg aagaagggat
      121 gacatttact gcaaacttca acctcaattt tttcgagagt agaaccggta gtaaatttat
      181 tgccgcaggc acaagtgact tggcagtctg agtgatagat aggatgaatt ccttgtttca
      241 tagggtttat tttaacacaa aattaatgat ttcgttttgg gaaatagttt tttgctcacc
      301 ggtagacatg ttcttgatag agacggtgtt gttaatgagt tcattttcac cgataagagc
      361 aacgaaggga atatttttaa ggctggcgta tttaaactgc ttaccaattc tttcctcatc
      421 cggataaaaa acagtcggga tattgttttg acggagaagg gaagtgagct ctaaagattt
      481 atttagagtt ttttcgccaa aattagcaac cataattttt gtcttagttt tgggtaaatt
      541 tggcagtagg attaattcga taatgcaatc aacaatacga tcaagaccga gagtggtacc
      601 gacagcagga atatcgggcc cgcccagagt ttttattaaa gtatcgtaac ggccaccacc
      661 ggtgatggaa ccaatttttg gttcttcgac gtaggtttca aaaattgtgc cggtgtagta
      721 atcaagaccg cgaaccatgg tggggtcaaa ggtgtaagac cagtcgggga gattaaaaga
      781 tttaatggcg gtaaaaacag cggctaaatt ttcgtcaggt ttggctactt tgatatattt
      841 aaacagagaa tcaatttgaa gctgagaaaa gccttttttg ttgagttctt ttcttactcc
      901 atcttcgccg attttctgga atttatcgag actttgaaga gccgaaattt gattttcaga
      961 aatgccgctt tgagataaaa tattttttaa aacagtgcgg ctatttaggc ggatagaaaa
     1021 ctttttaaaa tctaattttt taagagccgt gtagatgacg gcgacgattt cggcatcggc
     1081 aatgggggat ttggtgccaa aagtgtcgat gtcgcactga agaatttccc gataacggcc
     1141 tttttgagta ttgtcagcgc gccagacatt ttgaagttgg tagcgtttga aggggagggg
     1201 aatttccgga tacatggcca aaacttttgc cgtaggtacg gttaaatcgt aacgaagacc
     1261 aacgcggcgg ccgcctttgt cgttgaacat gtaaactaat ttttcaccct cgtcaccata
     1321 tttacccata agggtagaag cgtattcaag agaaggggtt tccagaggct cgaagccgaa
     1381 agtttcaaaa gtttcgatga agagattttt gacataattg cgaacggcca tggtctcggg
     1441 caaaaaatcc cgaaaacctt ttaatgtttg aggtgcattg ttattcataa gtttatttta
     1501 acattgtaag aataattaaa ttttttgaat gatgtcgagg agaatatttg gtaatttttc
     1561 caattttttt atctccacat actcattgga agagtgtgct gtttgttgtg taccagcccc
     1621 aattgttaag cattttggct gattaaatat ttgccaaagc atttggatat ctatataacc
     1681 tgaagtgcta atgtttttaa aatccaaacc gagtttggtt aactttgatt ttggggttag
     1741 ccaagatcca aagtcaaatt ttaccttata actggataat tctcctctca attcggtaat
     1801 atattttttt aactgattaa taattaattg ggcactaatt tttgaagagg atggccgaat
     1861 gtcgagggta aattggcagt tgtcaggaac gacattagaa gagctgccac aaccttcgat
     1921 gaaggctaaa ttgacagaag tagtgccaag ttcaggatcg acagattgag aaagaaattc
     1981 tttcaatttt tcgattgatt tagtagtcac ctcgatggca ttaatgccat ttttgggagt
     2041 agccgcgtga caactaatac ccttaactgt ggctgaaatt tcaattaaac cacgacaacc
     2101 attggcaatt tctaattcag aaccatcgag agaaataatg gaccttggtt tgattttaga
     2161 agcatagtcg gctatgaatt ttttaatgcc ggcgaaatca tattcttcgt cgatatagaa
     2221 aagaaagcca atattttttg gtaaattttt gtcggtagca atgagcacca tggcagcgag
     2281 accacttttc atgtcagtag taccacggcc aaataactta ccgtccacaa taatcggatt
     2341 gactggatca gtttcccaat tattgtcgac gccaactgta tcaatgtggc ctataattag
     2401 ggttttaaca ttttggttaa aacctaccag gatgttaaaa cgaccatcag ctactatttc
     2461 tttttcaatc gaaagttttg aattattttt taaatattca aaaataaatt ctccaatttt
     2521 tacctcgtta gtttgctcat tgacccagga ggggatggag atcaattttt gagtcagttc
     2581 taaaattttg gtcatgtttt aaataaaaaa ttataaatag agataataaa aaaatccccg
     2641 atttacgggg atttatagat aaaatagatt gcttcgctta agctcgcaat gacattatgg
     2701 tcaagacaac accccgccgg ttggtgacca cggtatgtgc atgatgtata aaaataaatt
     2761 ttataaacat atttttgggg tatcctatgc ttaaaagctt tggaatacct ttgggtagca
     2821 gaagggactc ggaccctcga cctcatcctc cacagggacg cgctctaacc aactgagcta
     2881 ctgccaccaa aataaaaacc gccttctatt ggcggttttg gttaattgat acagcaatta
     2941 ccgccaaatt aattggtggt atggtgatgt ttggagatca cgtttgcttg tatcattggg
     3001 agtattgtag caactttatg agtaaagtca attaattgag agtgataatc ttgagggcgg
     3061 ggtaggtaga attatcattg tttgtatata tacacactat tttttgacct ttcttgatgt
     3121 cggtaaattt caggctttta ttgtctttgg tgactatttc agttttactg tcttgtttca
     3181 tttgtagctc acggctgtta ttgttgatgg gaataagaac aaagacggaa gtggtagtgg
     3241 aaatatcggc aattttacca atagcaatat tttttttatt ttttagttta gtgggatcga
     3301 cgatgagtat ttttttggca tagacaattt tatctttggc taaagttaaa actagaacat
     3361 tttgaccttc tttgaggtca gttattttaa ttttcatttg ttttagattg acaaaggtgg
     3421 catcttcact aattacaaat tcatattttt gaccgctgga atctagacta agattatttt
     3481 tttctaattt agtaatggtg ccaagatagg cctttttggg attgattttt tcttcgccac
     3541 caatttcctg aagtttttgt tgaacttttt gttgaattac ttcacgtaat ttttgaatat
     3601 cgctttcggg tgttggactt tgggcgctga cagaagaaac aaaaaacgag aagccggaaa
     3661 ctagaaataa tataaaaaat attttctttg tcattctaat ttggtggtgg aataggtaag
     3721 aaataattcg aggtggtttt cttgatcatt ctcgtcaatg gtggttaaat taatgtcgtt
     3781 gtagcccgct tctaaactaa tttcttggga aaaaagaccc tgaatatcag ttgtcaaatg
     3841 atagtttttg agaggagtag tgataattat gagactattt ggacttgatt gaccttttat
     3901 ggtgactttg ctgatggaaa taatgtcgtt gttttgagga taatttactt ttagctgggg
     3961 aagtgaagaa tactcagaag taggaaaggg gtttgaggaa ggggaattat tattttcggg
     4021 agcagtagtg ggaatgacga tattaggggt gtttttctgc tggtttttgt tttgtttaac
     4081 gtaaaaataa gttccggtta aaccaaaacc aattaggagg ccgaggacaa tcgctaaaga
     4141 taattcctta atcatagaat gatttaatgt tatcataagg ctatggaaat aaaatatttt
     4201 aatcaaggca agatctattt aaagggtaaa aaggaaaatg tttgggtaaa tcctgataaa
     4261 gacgatttac gatcaaaaaa ttgtgaagcg agaattgtaa tctatactga taaacagcga
     4321 aatatcataa aattaggtga agaggggggg cgagtggtaa ttgctggacc gggagaatat
     4381 gaaatcggag ggatagaaat taatggtatc aattcaatgt atgttttgac cattgatgga
     4441 attaaggtgg tactaatggg taaagaagaa ggagaaatta gcgaaaagaa aaaagaaaaa
     4501 ctggaagagg cagacgtact tatgattagt gtccaggcag ggagtgcgca aattgccaaa
     4561 aaatcggcgg ctaattatgt gattccaatc gaatacgaaa ataataaaga agaactcaag
     4621 gagtttttag atatttttga cagtgaaaac ttagaaagtg ttgatagcct cagggtagag
     4681 aaagaaaatt tacccgaagc catggaggtg gtcttattaa agacgaacta gtaaaaatac
     4741 caccctcttt tgaggaagca gaaatttgtg tattaggttc gattttaatt gacaatgatg
     4801 caattttatc cgtttctgag tttttaagac cagagcattt ttataatcat aattatgctc
     4861 tgatttataa gaccatgatg gatctttatg aagagagaaa tccgattgat attgtgacag
     4921 tggcgggaaa gttaaaagaa gaaaaactat taaaaaacat tggtggtaag gctttattat
     4981 caaagctggc gaatgaagtg cctacttcgg ctaatgttga aagctatggc cgaatgatta
     5041 agggactctc ggctaaaaga gaactaatct cagtggcggg gagaatttcc cagaaagctt
     5101 ttgatgaatc aatgccagtc gagcagttac tcgatatggc tgagcaggaa gtctatgcac
     5161 tatctcagcg gcatatgaag tctgtgcctt catcgctcaa agaagtgctg acggctagct
     5221 ttgaccgcct ggacgagctt cagaagcggg gaagtggtct aaggggtctc tcaaccggat
     5281 ttcgacaact tgattcaatg ctggccggaa tgcaggatag taatttactg attttagcgg
     5341 ctaggccagg tctgggaaaa actgcttttt gtttgaatat tgctcggcat gtagcggttg
     5401 aagaaaaact accgacctgt attttttccc tagaaatgag taaagaagag ctggtagacc
     5461 gcttactggt aagacagggt ctaattgatg cctggaaatt aaaaacggga caactttcag
     5521 atgatgattt tacatcctta tctgaagcta tgggaatttt ggctgaggcg ccactttata
     5581 ttgatgacac ccctggcctt tcggtgactg aacttagaac caaggcgaga cgattgcaat
     5641 ctgataaggg gataaaattt attattgttg attacttgca attaatgcga ggacaatcga
     5701 gagaaaatag ggtccaggaa gtatcggaaa tatctcaggg tttaaagaat ttggccagag
     5761 agttaaaaat cccgatttta gcggcggctc aactaaatcg acaaatggaa accaggggag
     5821 gtaaaccgag actatctgat ttgagagagt cgggaagtat tgaacaggat tcggacgtgg
     5881 ttatgttttt atcaagggaa gatgatgaaa taaaggaagt cgttacttgc agtattgaaa
     5941 aacaccggaa tggaccaaca ggacagtttg atttgtactt taatgggaag caagtcagct
     6001 tctttaatgt cgagaaaaag gggtagattt catgagataa tatgaataat ttagcaggcc
     6061 gacgtggcgg aactggtaga cgcgcacgac tcaaaatcgt gtgatggtaa catcgtgtgg
     6121 gttcgattcc caccctcggc actcagatga ttaatttaat tttagggatt gtagcaatag
     6181 ttggctatct ttacttgagc tggcggactt tacgcgataa ttatcgggaa gaagatattg
     6241 tggctttttc ctgggtggct ttgttgtttt ttttagtggg agggaggctg acttatggtt
     6301 ttgaacactg gggagactgg gccgggaggg ggattacttg gctcgaattt tggaaaattt
     6361 atgaatttaa tatttggggt gggagtactc tctggctgag ctttgcgctt ttgatctcaa
     6421 aagataaaga ctggaaagct tgggcttttt tagagaattc tttgttaagt ttttggtttt
     6481 tactgaccat gtttggggta attacccgag actggaaatt gttgctggct ttgagtggag
     6541 cctatggatt aagtttattg ttaaagaaga aatatcgttc tttgatttgg tataaaagcg
     6601 gaaaaaaagg ctttctcttt ttttggtttt tcttttgttt ttggttaata tttactttta
     6661 ttaccaagat ttggtggtta cttgcttttt gcttgctttt tgggatggga ttgtttattc
     6721 taggtgatga taagttttcc aagtagacta acgaggggga ttgaggcttt cttgcatcac
     6781 aaactcattg atttaaaaaa gaaagaaaaa tctttaaaag aaagtgatcc tttttttgat
     6841 cagcagagaa ctggcaataa ttcacttgag gaagatgttg atgagcaaat tggacgtttt
     6901 gagactgaag ttaaaattag ttttattaaa aagcaaattg ttcaggtcag aaaggcttta
     6961 actaatctaa aaattggaaa atatgggata tgtgaaaaat gtcataatat gattgatact
     7021 gatcgcctcg ccgtaaaacc ggataccacc ctgtgtgtaa agtgtgccaa agacaaagaa
     7081 agctaatgat catcaataca ggagctagtt tgggattaaa ttttccattt ttaaaatgga
     7141 ttcagctgtt gttattgata attttggggg cggtttggct gagagacaaa aaagcttggg
     7201 gctggctact aatgataatt gggggcggac taaatttaat tgaaagatgg cagctggggg
     7261 gcgtaagaga ctactggcct cttttcggaa cttcaatcta taataatatt aatgattatt
     7321 taattgcagc tggaatggtc cagctaatag taatatatta tggaaaaaaa ggcaaaaata
     7381 atatatcagg ataaagacat tttggttata aataaaaaaa gtggggtagt ctccacaaaa
     7441 gaggggagaa aggataagga aacacttgag gactggctga tccagaaatt tggaaaaaat
     7501 gatttgccga ggcagggagt cgttcatcga ctggataagg gcacttcagg gctgatggta
     7561 gtggttaaga acaaagaaac gagaaataag attttagaaa tgtttaagaa aagagaaatc
     7621 aaaaaaacct atctcgcctt ggttgagggt gacttgccga aagatggaga gataaatgtc
     7681 cccattaata gatcaaaata tgtctttggt aaatttgcgg tgagtgaaga tggaaaggtg
     7741 gcggaaacca gttttaagct cttaaaaaaa tattaccggg ataataaagt ttattcttta
     7801 attgaaatcg atttaaaaac ggggaaaact caccaaatta gagttcattt tagttatttg
     7861 aagtggccac tnnnagttta aacatcccga gacaggagct aatttaagct ttgagggaga
     7921 gctggcgagt gatttaaaag aattactcgc aaaattatga aaaaaattat attaatctta
     7981 ataattatcg caatttttgg gtatttattg aaaaaatcat ggcagaataa tgattttaag
     8041 ctgggaatta ttaccccaaa tcaaatcgct cttctttcaa tttcacccga aaggggcatg
     8101 ataaacctgc taagtgttga ttctgaagtg gaagtctggc tacccggagg aatggggtgg
     8161 tatccgagca ataaaatcct gaagatttat caagctgata agaatattaa attaataaaa
     8221 cagacttttt attataattt tggttttatg cccgaaaata ttgttgtttt agatgaggta
     8281 ggggattggc gaagctggga tttgataaag catctcggtt ttttaaactg gattcgttat
     8341 ctttttgagc aaggtagctg gctatataaa accgacacat taaccagatc aatggagatg
     8401 gaaaaagaaa aactagatga gctattgccg agagattttg ctgaaagtga actgctaggg
     8461 caagagctaa aagtgacagt aattaatgac agtggggaaa acaagcttgg ttcttttgta
     8521 gctgatagat taaactggat gggatttagg gtaatggagg tcagaagtga gcaggcaaaa
     8581 aaggaatgtg aaattatgtt tcggattgga agtgaaggga taattaaaaa atatgccgaa
     8641 atactggcta aagaattctc ttgttctttg attagtagtg gaacacttct ggaaaatgaa
     8701 atggttttat atttaggtca aaactatagc tcgatgataa aatacaatag ctatgtcggg
     8761 acattctaaa tgggcaaata ttaaaaatcg gaagggagct caggataaaa aaagaagtga
     8821 agcttttacc agagcttcta aaaatatcat gacggcgatt cgagaaagtg ggggaaattc
     8881 taatcctgag tcgaatatta gtcttcgaga ggcaatagac aaagcgaggg caattaatat
     8941 gcctaaagaa aatattgaaa gactgctcca aagatttgaa gaaagaaaaa ataacctagt
     9001 tagtggcgtt tttgaaggtt ttggcccttt cggggtgcca gttatgatag aggtagaaac
     9061 agataataaa aatagaatcc tgggtgaaat taaattaatt tttagagatt atgaagggtc
     9121 tctcggtgaa agtggcagtg tcgcttttat gtttaaaaga gtgggggaga ttgagcttga
     9181 gtctaaactt gatgaagagt cagagctaaa attgatcgat gagggggtaa aagacataaa
     9241 agataatctg gtgcaaactg aagtgaatga ctgtaaaaaa ataggagaga agataaaggc
     9301 aatggggggc cggattaaaa aaatagggct tgtttatcag tgcttaaatc cggtcaggct
     9361 gaatgacgag actaaactgg aaaaaatact cgatatgatt gaagaactgg aggataatga
     9421 tgaggtgatt aatgtttttg ctggttttga ctattatgtc caaaagaatt aaatatttta
     9481 tttcttcgct aattggagct ttggtttttt atttatatct aggtttaccg gtagaatcca
     9541 aatactatgg tttaattttg atgctggtaa tcgtggtttt ttgtttttgg tttggcctag
     9601 ggataatatt tgaaggtaat tttgaaaccc gaattatgcc agtattgctg ccgattttat
     9661 ggacaatggg ctttggctta ttttccgtat tattgccctt aaactggcta aatatattac
     9721 ttattactat tttttttgga gttattttat atattatctt tttggtggaa aatatttttt
     9781 tggtggcgat tggctatcga actgtcccgc tttatcgggc tgcttacaca gtcagtctaa
     9841 tattggttct attaatatct ttttttgttt ttgattcttt attttccttt aagtttcctt
     9901 tttggggaaa tatgctggga acaataatcc tgggcttatt aattttctcc tatcagtatt
     9961 gggcggtggc aattgaacta ccagatgatg gtaaggagaa gggtaaatgg ccttatattt
    10021 tagttcccac cttaatactg acggaattgg ctggtatatt gtctttttgg ccagtgggaa
    10081 tatttaaagg ctcaatttat ctggttgcgg ctatatatat aattactggc ttattacagg
    10141 cagaaattag agatagactt tttaggggag tggttttgag ttatagctat attggtgtgg
    10201 ctgtgatcct ggcgattatt ttagtaacta aatggggata agataaaaaa agtctattaa
    10261 atagaatata aatgagtggg cccgccttcg taaaatacta cggcgggtaa agaagtaggg
    10321 aagtgggttt tcacgatata tcctataatt ttcacaataa ttataggata ttataggacc
    10381 tactactgat atagtatgat atagtctgtg tggaaaacta tttagcaatc gacgatatca
    10441 cttctcaagg caagagtggg ctctaggaga gagataggga gagagaaata aaaagaaata
    10501 aaaatctaaa ttataaaatc taaagcatga gaactaatgg agaaagatgg ttactcccca
    10561 ccagaggggt agttgatttt aatatgtcgc acaatgtatc ttttacgact atacaaaagc
    10621 gggaagtgga ggggagaggc aaaaaataaa ttttcaatta tcagttataa attgcgaatt
    10681 attaaaatat ttcatatttt gaaccgtaaa ataaagtctt taattcctct cttacgctcc
    10741 tggctaccca aaatctgtct ttagcttaaa attccttctt ggctacttcc ctggcctgtt
    10801 ttaaagaggg tatttataaa ttatacctaa aataccgtta tttatatata tggcttcacc
    10861 agaagcccgt aaacgtacgt tttatatttt tggatgttag aataagatat gaatttagat
    10921 ttagcgacag gaatcagaag atttctcgaa catttggagg tagaaaagaa ctgctctaaa
    10981 ctgacaatca gaaattatca acattatctc gaagttctct tgaactacat tgagaaacag
    11041 aatcagaaaa ctcccgaggt gtctgacatt gaccaagaga ccattagaag ctttagattg
    11101 tatttatcaa gacaggctgg gattgacggt gaaatgaaaa ttgtaactca gggctattat
    11161 gtaattgccc tgagatcttt tttaaaatgg ctgattaaaa acgattatga ggtaatgcag
    11221 ccggaaaaaa ttgatgtgcc taaaaataaa gatcattctt taaagttttt aaacggtgaa
    11281 caaatagaaa gattgttaaa tcagccctta ctttcgacaa aagttggtat tcgagaccgg
    11341 gctattctgg agcttttgtt ttcaactggg cttcgggttt cagagcttac aaggcttaat
    11401 cgtgatcaaa ttaacctaaa tacccgtgaa tttggggtaa ttggcaaggg gggtaaggcg
    11461 agagtggtct atattagtaa aagagcggta atttacgttg ataagtattt aaaaacaaga
    11521 agcgacaagg ataggcccct ctttattcgc tacggaggga aaaaggaaat tattaatgaa
    11581 gaggaaaaaa tgaggctcag tccccgctcg attgaaagaa tagtcaagca ttatgtacgc
    11641 ctggccaaat taccggttga tgccacgcca cataccctca gacattcgat ggccacggac
    11701 ctgctacaga gtggcgctga tatcaggtca gtccaggaaa tgcttggtca taaaaatatt
    11761 gctacgactc aaatttatac ccatgttacc aatgcccggc ttcgggaaat tcatgataaa
    11821 tttcattcag gtaataaata aggatgtgta acaggtatgt cctatagttt cgatattttt
    11881 aagaaagatc ggggaaatct tattttgact actcccctag cctattgaaa ttgtgttttt
    11941 gcctatttta agactttatt aatttttcaa agctggttag caaggtagaa ttttgactgc
    12001 tattaaagct aaattttatt taggtatcct ataattatta tatagttata tcctatattt
    12061 tttggggtag ctccccttct ttctcatttt tctgtaaaac cgtcatggct gagaccttgt
    12121 cgtcattttt aagtctcatt aaaataaccc cacgggtgtt gcgagacagt gtcggaatat
    12181 ttttgagagg aagcttgatg gtcaccgctt ttttgctaac aaagagaacc tgttcatcgt
    12241 tttccgtgac gactaccgta gcagctaaat tgccagtttt gctcgtaaga ttggcaactt
    12301 ttacaccaaa accgccccgt ttttgaagag gatagagata aacgtcactc cgtttgccga
    12361 ctccattttc agtaatgact aagaggtggc ggaaaaattt tctcttcttt tcttctttaa
    12421 tggccagaac actttgagga agaacatcaa tgccaaccag ataatcattt tctttgagag
    12481 aaattcctcg aacgccacga gtatgtcgac ccatggggcg gcagtcaact tcattgaaac
    12541 gaatgcattt accggcgtga gtggccataa aaatttggtc attgccggag gtaagcttgg
    12601 cccagattaa ttcatctttg gggtcgagct tgatcgcggt gagtccagat tgacggatat
    12661 ttttgaattt ttcgatggca gtttttttga tggtaccttt tttggtggct aaaaggatgt
    12721 attttgccgg attattttta ctgatgggta aaacagcctg aacgatttca ccttgaatga
    12781 tattgattaa attaatgaca gcttggcctt ttgattgacg actactttca ttgatatccc
    12841 aaacttttat tttaaaacag cgacctttgt tggtaaaaat taaaagatcg tcgtgagtat
    12901 caacgaaaag aaggctatcg acctcatctt cacttttggt ggccatcccc ataacaccga
    12961 caccaccacg ctgttgggtc cggtaggttt ccatgggaac tcttttgata taaccgccac
    13021 gagttagagt gaccaaagtt gcttcattcg gaattaaatc ttcatcactg aactgctcaa
    13081 gccccttggc gtaaatttta gttaaacgag aatcggcgta tttttcggta atttctttgt
    13141 tttcagattt aatcatggct accatttttt caggaatggt aataatggcg gttaaattgg
    13201 tgatggtttc ttcaacttct ttatattctt cgtcgagttt ttgacgctcg agcccggaaa
    13261 gctttttgag ttgcatttct aaaatggctt gggcttgaag atcagtaaag ccgaatttat
    13321 ccatgagctt ggtcttggca tcttcatcgg gttttgtgcc tcggatagtt ttgattattt
    13381 cgtcaataac atccagagct tttttgagcc cttcaaggat atgggccctc atataagctg
    13441 atttgagatc aaaaatactg cgacgacgga tgacattttc ccggtgtcta aggaataaaa
    13501 gtaatatttg gcggagatta agagtctgag ggataccatt aaccaaggcc aataaattgg
    13561 cagaatacga tgattgaagg gcagtatatt taaatagttt atttaaaatg gctttgggtt
    13621 tgccgttctt ttttatttca ataacgaccc gaattccgtg acggtcagac tcatcccgaa
    13681 gatcagaaat accgataatt tttttgtcgg taaccaactg ggcgatcttt tgaatcatct
    13741 cggacttgtt gacctgatag ggaatctcgg taatgataat tctgattttg ccagatttag
    13801 tttcttcgat atttgtttta ccccggagag taaatttacc ccggccggta gtgtacattt
    13861 ctaaaatacc ggttttaccg taaatttcac cggcagtggg aaaatcgggg ccggtaatta
    13921 tcttggtcag gtcctcgatg gtagcggtag tttcaaaatt aaaagataat ttttgaagct
    13981 cacggagagg ttttagaact atttcctgag tactaagatt tttctctaaa agagggttaa
    14041 tttcaatttc attttcctgt ttttcaaggg tagcgtgatc taaaataaag tcgagtccac
    14101 tacagatttc ttttaaatta tggggtggga ttttggtggc cataccgaca gctataccgt
    14161 cggcaccatt taagagcaaa ttgggaattc gggtgggtag gacttcgggc tctttgaggg
    14221 tagcgtcgaa attatcgacc atggaaacag tgtctttttc aatatcagtg attaattccg
    14281 gagaaatttt actcatcttg acttcggtat aacgcatggc ggctggaggg tcgccatcaa
    14341 tagaaccgaa attaccctga ccattaacaa gggtgtatct tagggagaaa tcttgagcca
    14401 gacgtaccat ggagccataa acggccatgt cgccgtgagg atgatattta ccgagaactt
    14461 cgccgacaac tttggctgat ttggaatagt gtccaccggg agtcaaaccc atttggttca
    14521 tagcatagat aatgcggcga tgcactggtt tcatgccatc acggacatca ggcagagctc
    14581 gggcgacgat aactgacatg gcatagtcga gataggactt ttccatctcg gggacaatat
    14641 cgatatccaa aatcttgcca atattattaa caaaggcagg agtatcatta acaatagtgt
    14701 ttttttgttt atctatgggc ataaaattaa atatccaaat tagctaaatg ggcatgggta
    14761 acgataaact ttttgcgcgg tgggacttcg tcacccatta aaattctaaa agtttcgtcg
    14821 gctttttcgg cgtcggcaat cgagattctt ttgagaagtc ttgtctctgg attcatagta
    14881 gtttcccaga gctgctgagg attcatttct ccgagaccct tgtagcgctg ttggtcgtag
    14941 tttttggaag tatcgagagt ctttaaatag gcctcttttt cttcgtcggt atagacatat
    15001 ttgactgttt tcccttgctt tattttataa agtggtggca tggcgatata aacataaccg
    15061 tgttcaatta aaccgggcag atggcggaag ataaaagtca gtaaaagggt ggcaatgtgg
    15121 gcgccgtcaa catcagcatc agacattaaa attatccggt gataacgcaa tttctctata
    15181 ttaaaggtgt cgccaattcc catgccgagg gcaacaataa agtcttttaa tttatcggag
    15241 ctaacgactt tatcaagtct catcccttcg gtgtttaagg ctttacccca aagagggagg
    15301 atggcttgga atttacgatc ccgaccttgt ttggcgctac caccagctga atctccttcg
    15361 acaatataaa tttcacattc ttcaggtttt ttactttgac aatcggcaag tttaccggga
    15421 agacccatag aatcgaaggc acctttgcgc ataatggcat ctttagcggc cttggcagct
    15481 ttgcggattt ggatagtcaa aagggctttg ctgataatga ttttggcggt atcgggatgt
    15541 tcttcaaaat aagtatcgag ttgttctcta acaattttat tgacaatcgg ttggacttca
    15601 gaattaccaa gcttgccttt ggtttggcct tcaaactgaa gatctttact ccccatttta
    15661 atggaaagag cggcagtgag accttcacga acatcgtcac cagtaatggt gtcgtctttg
    15721 ttttttatca tttctttgcg ctcagcatag tcttttatgg ctttggttag ggcaatttta
    15781 aagcctgtta agtgagtgcc gccttcgtgg gtgttgatga tattgacata ggagggagtg
    15841 ttttcctgaa aagaatcgtt gtactgaatg gcgacttcga cattaacgcc atcactttcc
    15901 ccctgaatgt agatgggatc atgaatgacg tttttatttt tatttaattc tcgaagcagg
    15961 gataaaatac cgccttcaaa aaaataatgg tgttcgctat gattgcggtc atcataaaaa
    16021 tgaaagtaaa ttttgctggt taaataagct cgatctttga gtgatttaat aatggttttg
    16081 ttgtcaaact cggtagtttc tttaaagatt tctggatcag gataaaaagt agtagtggtg
    16141 ccggtaatgc cgccgggaag gatactttcg agtctctcaa ttttgctttt aggaatggta
    16201 gttaattttg ttttaacctt acctttggaa aattcgataa aatgagtttc cttttcccgg
    16261 cgaacttcaa cttgaaaaag agtcgaaaga gcattgacac aggcagcgcc caccccatga
    16321 agccctccag aaactttgta ggctgtgcct tcaaatttac caccggaatg gagggtggtc
    16381 atgatgactt cgatagcagg ttttttgtat ttgggcatga tatcaatagg aatgccccgg
    16441 ccatcatcgg cgacggtagc agaattgtcg ggatgaatga taatccaaat atttttggca
    16501 aagccagcta aagcctcgtc aatagaattg tcgatgattt cgttgagaca gtgatgaaga
    16561 ccagtggtat cagttgaacc aatatacatg gcgggacgtt ttctgaccgg ctcaagaccc
    16621 tcgagaacaa caatttgttt agaagtataa tctgtggttt ctggcatatt gaaaaataaa
    16681 aaaatccaaa gtctaaattc taaaatattt agaataatta gggataaatt aattgtagca
    16741 gataaaaata gcttgacaat aagcaaatat gatggaaaga taggccaaat aaaatgataa
    16801 aaccatatca aaataggtaa aattaagtgc taatttatag ccttaaataa ggtaaatttt
    16861 ttgtgttaaa atagcctaat gtcggttgaa ataataaaaa gtgctttatg ttcacctacg
    16921 ggcgatttaa tgccagatga aattggttac agtcctgagt tgtaccgaag acaattaatg
    16981 acagtaaatg cttcaagaaa taatgtttac gaatgttctc tttttcttaa aggagaatgt
    17041 aagaagagtg gaactgaatg tcttcccaat aaaggaaatt tctttagagt aatagtttta
    17101 ccttagtttt attttactga tagcattgaa ggcgatgact gcggtggcga cgaggacatt
    17161 tagagaaata ttgatgccaa acattggaat ttccacaatt tgatcacaga tttttaaaat
    17221 atctttagag ataccattaa tttcgctacc agcgacgata gctaaggggt atttatactt
    17281 tgctttttga tagaggatgc tcccctcacc ttgctcgcag gcaacgaggt gatagccttc
    17341 tttctttagt tttaaaaggc attcctttgt agaagatatt tgttcccaag ggacccattt
    17401 ccaggtgcca atagaggctc ggtggatttt gatgttgggg ggggtaacga cggaaccaca
    17461 gagatatatt ttctcgactc cgagggcatc agctaggcga aaaaaggaac cgatattgta
    17521 ggtatccaga atattgtcga ggactaaaac tagaggctga cgctttagca gtttttgttt
    17581 tttcagtgac tcccctactc tagattttcg cagctgatga gaatttagtt tcatattttt
    17641 ttgccgacaa atctaattaa atttttgatg ctgtctttat atgtttcacc attttcatct
    17701 aaaatttcaa tttggtatct atcccctatt agttgacgaa gatatttcac cggaaaaaac
    17761 ctctttttaa ttcctaccgg actgagaaaa tatccttctt ctattttttc tgattcggca
    17821 acttccggat cattgattgt attaactaaa atggccaata taccgttttt ttttaagatt
    17881 ctatgtattt cgttaaatat gttttgagtt gtgatgttat caaaataatg tagagataaa
    17941 tgagaataaa cgatatcaaa agaagagtcg tcaaatggta aaggttttgt taaatcaaca
    18001 tttttataat cgatttctaa attattagat ttgtttctgg aaatatctaa agcatcttgg
    18061 gtaaaatcag tgctggtaac ttgaaaatta ttttgggcaa agaagcgact gtcttggcct
    18121 tgaccggcgc caagatcgat tagctttcca ttttttggta aaagattcac tatttgttca
    18181 gcaaaaatag ttggtttggt tatccaatca taggtggaat aattttggtg ttgcttttgc
    18241 caatagtcat gagatttcat aaatatattt tacatcaaaa attacctaaa attgggaata
    18301 aaaatttttt aaatttggtt ggttgagttt gtattgggaa taacgggtct aattgtgttt
    18361 ttgtagagac gccatatatg gcgtctctac aaggaaagtt cggaaattga atactaaata
    18421 aatagttgtc gaaacaacca atggtgaaat aattattatt atatcaaaaa atccccctat
    18481 tacaggggga tttttaaaat ctaagattgc ttcggattat ctcgcaatga caggttgaga
    18541 ttagcatccg ccggcgggca cagcttgacc ggtcaggtca ggaatttcag ttttacactc
    18601 ggtgggactt ttttggaaag aggtacagag ggcttcttta attacatttg gagtccggta
    18661 acgatcttgg gtggccactt tactgccaat tttaagagaa cctttgccgt cttgagtgta
    18721 ggcagtttct ggggggaagg agacatcatt aatgagaact gtgggagaac ccgaaacatt
    18781 atatttggta gttagggcaa tttctttctc gataaggtct attccctctt tattaaaaca
    18841 ttcggtaatt ttggctgttt catagccagc acttttagcc tgttcttccc agcaattatc
    18901 aacattagtc gaggtacagt tcttgttgac attaccaaca aaattccacc attgagtttt
    18961 gtcagtagct aaattccagg cacaaatttc ccgaacatcc tgattagctt cttgacggcc
    19021 atggagagag gcgtacatgg ctcctttttc agattttata taggcacttt catccttaca
    19081 gtcggataaa cttttggcga tggctttttg acagtcagcg acggtgctaa agtatttatt
    19141 ctcgacataa gcggcacact gagtggcatc gccagaacgg cttttgcaat aggtgtcgag
    19201 attttcgatt ttatcaaaaa tataatgagg ggtgatatca gcactatctt taagtaagtc
    19261 aaagacggga cggagaacat cctccatttg attaccgtag ggacaaaaac tcatgacaaa
    19321 aaattttaat tcaggttttt ggc
//
LOCUS       DHGB01000037            4241 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_62501, whole genome shotgun sequence.
ACCESSION   DHGB01000037 DHGB01000000
VERSION     DHGB01000037.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_62501"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1265 a          720 c          919 g         1337 t
ORIGIN      
        1 caagtaaatg gtggtattta tttaagcgaa ggtttagact ataaattatg aaatttagac
       61 cagcattttc cctcggttct tttatattag aatttgtcca atcaatagta ttggcattat
      121 ctgtttttgt tttaatttat ttatttgtag ctcagccaaa ccaggttaaa gggtcttcaa
      181 tggtgcccaa ttttattgac ggggaatatt tactgacaga taaaatttct tatcagtttg
      241 gagctcctca aagaggtgac gtcgtcgttt ttaaagcgcc tcctaccgag ccctgcgctg
      301 aggatgagtg tgaatatatt aaaagaataa ttgggttgcc aggtgataag gtaatggttg
      361 aaaatggcga ggtttatttg aatggaaaat tactggagca atcatttatt cctgatgact
      421 atatgactga ttccggttct tattgccaag agggcgttga ggttgaggtc cctgatggca
      481 aatatttagt ttttggagat aaccgatctc attctcgtga tggccgagaa tttggtccga
      541 ttgataaatc tttgattgtc ggcaaagcct tttttaaata ctggccagtc agtgaaatgg
      601 gtctaattcc cactgtccgt ttttaggtct ttcttgcaaa tttctttata aagctctccc
      661 agttgttcaa agcgtttttc gactgggcct tgaaattgaa atacaacgcg ggtgtaaata
      721 ttagtttggg aaattttgac tcgagtggtt ttattatcaa aaactgattc taaatcacta
      781 ctaatattgt cgaggcgtga ttttaaaaat tggggaatgt tttccttatt gatgggaatt
      841 aacccttcgt caattttttg tctcatgcgg cgggctattt cttcagcccg gcggactgaa
      901 gctttttctt ttagaatcac cttgtacgct tcgatcatta aacgggtgtc gccaatgcca
      961 ctgagggctc tggcatgccc ctcagagatc agaccggaga gcagtccatc cttgatggca
     1021 tcgggaagac tcagaagctt taagacatta atcacgtaag gaacactttt actaactttt
     1081 ttggcaatgg cgtgattatc gagtccaaat tcttctttga gccttaaaaa ggctttagct
     1141 ctttcgatgg gatttaaatc tttacgctgg acattttcaa caatcgccat ttccagcatt
     1201 tgctggggag tggttttttt aataattgct gggacgtaag gtaatttaag gattctggct
     1261 gcccgccagc gtctctcgcc ggcaattatt tgatagccag ccggagtttg ggcgataatt
     1321 aagggctcta aaataccatg ttctcggata gattcaacta gttcagaaat tgagtctgag
     1381 ttgataatac ctcttggttg aagtggattt ggttcgagct gattggtagg aatttgggta
     1441 atatctttca tagctccagg ttactatgaa aaccctcgtg aaaaaagaga gatgaactat
     1501 atcaaaattt aggctttgat gactgaaaca acttttttac caaatttagt gaagaatttt
     1561 acgatacctg gtctaatagc ataaatagtg aaatctttac ctgttttagt cccggcaccg
     1621 ccatgaaatt tggagccaac ttgacggaca attatttcac cgactttgat cttttgatca
     1681 ccgaattttt taatcccccg cctttggccg ggacgatttg atttttgatt ggtttttcct
     1741 tgggatttgg tatgtgccat ataatttaat tatcaatttt caattaacaa ttatcaatta
     1801 aaatttaact tggtaatttt aatgtcggtg agttgggcgc gaaagccttt agtttttcgg
     1861 taacgggatt tggctttaaa ggtcgagact cggagttttt cgccttgata atctcgaaca
     1921 ttttcaaatt caactgaagc gtctttgacg gtaggattgc cgacttttac tttatcgcca
     1981 tcgacagtta agagaacctc aggagtagat ttgattgaag gatccaattt atcaactgta
     2041 attacttggt tttcttctac tttgtactgt gagccggaaa tggcaattac cgcatatttc
     2101 atacttttcg attatatcat aggataaatt tttaggcaga gtaaataatt cccagagaaa
     2161 aaagtaaaga catgattgaa gaaccaccaa ctgacataaa aggcagggga atgccggtga
     2221 ccgggagaag accaagattc atgccaatat tgataaaggc ttgagtccag atttcgatag
     2281 caatgccgag agtaaagata aacaaaggta aattattgtg agtattgtag gctttttcga
     2341 ttagggtttt gataataaaa aaataagcca aaattattag ggaaatggcg aaaaaaccaa
     2401 gctcttcagt cgtggccgca aaaataaaat cagtgtgttt ttcaggtaaa aataaaagct
     2461 gggcctgagt tccctggcga taacccttac caaataatcc tccagaacca atggcaattt
     2521 gagattgaat tagattataa ccctgaccaa gaggatcggc agtgggattt aaaaaattaa
     2581 caatcctttc tttctgatag tcccggagaa caaaacggta ggcagcggga gagagaaaaa
     2641 ctaaaataat acctaaaaat aggctaagtt tgagaatttt tggattataa aatatggctg
     2701 gtagcatcaa gactaaaata gtgatggcag acccgagatc tggctgaagc ataacaataa
     2761 aaacaggaat taataaaaat aagggctgac tcgtggtggc caaaaataac atcagaggag
     2821 ctttcatgat ttcgcttggt tgtagagtta aaaagccgat gttaatccag cgatgagagc
     2881 ctctggtcaa atttcctaaa attataggta gcatcaacat aaaacaagca aaaaagaaaa
     2941 gagaccagcg gtagggctga ctttgtttgg ggtttatttg gagaccgata aaaaataaaa
     3001 taattccaat aatccaggag aggatttgtt ttaataagag tgaaggagcc agagaactaa
     3061 tcatgagtag attaaacgaa caaagaacaa taagactaat gacaagagct agaggaaatt
     3121 tatttctcat gaaatttttt cgacacttag tttatcccct ttttcgattg aatcggccag
     3181 ggttttagct aatatctgtg attcataatt tttaggccag cctggagcag taattttgat
     3241 ccggtctttg atctcaaagc cctgattttt tcggagaact tggatatttc gaacaagatc
     3301 acgaaattca ccttcggcgg aaagctcagg ggtgatgttc gtatcgagga ttactttagt
     3361 gtttttttct tgactgatat taactttttt gacattaagc tcctgagaca taatttcaga
     3421 gagactgttt agatactcct gcgaaatcac cacgggacag caaatggtta tggttgagag
     3481 aggttgtcta attttgatac aggctttttg acgttcagag tggccctgtt ggcacagatt
     3541 tctcaccagc tccatatcac tttccagttt ttcattaata tattctattt ggctatgtgg
     3601 ccaatcctga agatgtattg acgagttttg gtgtaggttc tggtataaaa cctcacttag
     3661 gaagggggta attggggcca taagtcgaaa aatattatca aaaacataga aaagaagaga
     3721 aaaattgtct gttctttccc gactccgccg gatataccag gtagaaagat cactgacgag
     3781 tttttcgatg gcagcggtgc tcttggcggt attatatttt tcgagattag tggtgactat
     3841 ttcgatagtg tgatttaggc gggaaaacag ccatttatct ataggctctg atgattgctt
     3901 cgcttcactc gcaatgacgt ctggtgttgg ctgccaagat tctaaattgg catgctgggt
     3961 aaaaaatcga taagagttcc agagaatgag gagaaaatca cgtttgacct gatcggcaat
     4021 gttgtaacca aaattgacac tagtataggc tttttgttga caataaagcc agcgcatggg
     4081 gtcagcgccc attttttcga tagcatcatc aaagggaata ccgttttttt tggttttact
     4141 cattcgctct cctttttcgt cacgaacttc agcgtgagta acgacatttt tatagggcgg
     4201 ttcattttca aaaacaacac taaagaaaag catggaataa a
//
LOCUS       DHGB01000038            2542 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_37385, whole genome shotgun sequence.
ACCESSION   DHGB01000038 DHGB01000000
VERSION     DHGB01000038.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_37385"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT          951 a          393 c          498 g          696 t
ORIGIN      
        1 aaaattaaag gaaaaaatag ggctgaccag gcttcattaa aggcgcctct aatgaaaata
       61 ttgacagcgt gatatggcgc ataagtgtaa aaaagaccag ctagaaaacc acctaaggga
      121 ccggttagat cactcgccag tagatacatc gctaaggcag atagagtaat cataagaatg
      181 gcagataatt tgacagtagt gacgaaagaa gaacctagac ttctaaaaat ttgtccagtt
      241 aaataaggta ggggtggata aaaattaaat aannggatag ccatagccgt agcccaagtc
      301 aggcgtccag cgacaaggaa tttggccatc tttaaagcat ttttccattt ccatttgccg
      361 aatcatctgc atatcatcat gcatattcca ataaaggccg ggtttcaaaa gaaaactaaa
      421 agttggaata attaaaatta ttagtagtgt cggccaaaaa aagcgatgat gaaataattt
      481 ggctagcatg cttctatttt actatgaatc cgcgcttata agcgataaag aagtaaagca
      541 tcaccatcag gctttggtat acttttaacc aaatttaggg tagaaatatc ggcactacta
      601 agttttaggc gagaggcatt aaaaagcata taaacttcat caccataatt tttggcatca
      661 attaattttt cgggaatttt ttcaatgttt atgcccacac caaagatggt taactgaggt
      721 agctgattgg tgtaaatttg aagaccatca ggnnccccag cccgaagtcc agtcaataat
      781 gtaaccggtt tcagtcgggg gcagttttaa attgtatggc cgaaaactaa tttgataaat
      841 ccgccataaa ttggggataa ataaaattaa tagaagatat atatattttt tattaaagag
      901 tggattgaga aagtgataaa aaactagaat taggggtggc agagtaaata aaatatagcg
      961 ggcagtaaaa actttggcca tggcgcagtt ggcaattaaa ggcaaaagaa accaagcaaa
     1021 ataaataagg aaaatagaat caaatttaat tttttttctg aaaggaaaat ataaaaatgg
     1081 cgagagtaat atggggatag ataaaaaata tttataaatg tttaaggtgt ctttgagatg
     1141 gggaataaga ggatcaaggg gatgttttaa aacttcagtt aatggccaga tgtaatcttt
     1201 atttcttaaa gctatttgag caaaggcagg accgagacgg agaatattgt aaatacaaaa
     1261 agcgataaaa gctgaaagaa ggggaaaata taattttttt agattttttg gattgtaaat
     1321 aataaaagtc aaaatgctaa gaactataaa ataaatggcc ggagatttgg tcaaccaagc
     1381 aagacctaag ataacaccca aaatcatggc taaatcaaag cggggatatt tagccagttt
     1441 aagtgataag aaaagagaaa atagacccaa aaatgagagt aaattatcag ccgtggctag
     1501 gcgatcaaaa aagaaggtga aaggtaaaaa aagataaatc aacgaaggta aaaaagaatg
     1561 gctaattaaa aacaaagtaa ttaatatacc tagccctgaa aaaacagaaa tgagtcgacc
     1621 tgaaatgaga gggtcagaaa taaaacgaag agtaacggca ttgagccaca taaagagggg
     1681 ctgtttacca tcggtttggg gaataaaacg gagagtatcg acacttttaa taatttgggc
     1741 ccagcgaatg tagatagctt catccccgaa tacaggaaga gattctagat tccagatcct
     1801 agaaattaaa aagataagac atataatacc taaaataagg ctatttttga gatttatttt
     1861 cataaacacc tttaagattg ttggtaaaaa ctcgccaaac ttcctgggcc attctctttg
     1921 attcttttag atatttggca ctggcatcac ccttggtcac gctggtatct tcatcgtgcc
     1981 ataaaaccgg aacttctttt attttgtagt ccattttgtc ggcaataaaa agcatttcaa
     2041 catcataggc agaaactcgc cacccttgac cactttgaat gtttttaatc acattaagct
     2101 gaggaaaaat ttctttggct aaagctgtct tcatagattt aaaaccacac tgggtgtcat
     2161 caatagaact ttgtaataat attttacgaa ataaagaaaa tacttttgaa cctaaaagtc
     2221 gaataaaact aaagccactt cgatttcccc ctcgagaacc aataacaatg tcatagccct
     2281 tatcgtaaaa agaaagaagt ttttttattt ctgaaattgg agtggactga tccatatcgg
     2341 taaaaagaac aattggatat ttagcttgtt gaaggcctcc ccaaatagct ccgggtttgc
     2401 ctccttttgg taaatcaaga acccgaaatt taggatgttt tttgacaaaa tcttgtacta
     2461 tttttagact attgtcggtc gattgatcgt tggcaataat gacttcccat ttaaaatcaa
     2521 ctgtttttaa aaaattggca ac
//
LOCUS       DHGB01000039            5861 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_10571, whole genome shotgun sequence.
ACCESSION   DHGB01000039 DHGB01000000
VERSION     DHGB01000039.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_10571"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         2030 a          783 c         1419 g         1629 t
ORIGIN      
        1 agtcaaatgt cgcaaacgag tgaatttaag ggtggtaaaa ttgactaaag atggaccaga
       61 aaatttattt atacaatact ttaactagaa aaaaagaaga atttaaaccg attgtatcag
      121 ggaaagtggg aatttattct tgcggtccaa cggtatattg gaatcaacat gtgggaaata
      181 tgtatgcttt tactatttgg gatacactgg tgaagttttt gagatatgtt ggatatgaag
      241 taacttgggt ggttaatatt acggatgtgg gacataatac gtctgatgct gatgaagggg
      301 aagataagat agaaaagggg gcaaaaagag aaaatttatc agtatgggaa attgccaaaa
      361 aatatgaagt tcaatatgta gatagtttaa gattattaaa tattaaacct gatatttcac
      421 cgcgagcgac agaacatatt tcagagcaaa tagagatggc tcaaaaaatt gaaaaaaatg
      481 gatacacata caaaacaaaa acaggcttgg tttttgatac tgggaagttt aagaattacg
      541 ctaaatttgc gaatttaaaa ctaaatgaaa tgaatagtgg ggctagagtt gaagttgatg
      601 aagataaaaa aagtccttgg gatttcttgc tttgggtaac aaatcaaata aatcatataa
      661 tgaaatggcc atcaccttgg ggagagggtt ttcctggttg gcatttggaa tgtacggcca
      721 tgtcaacaaa atatttggga gagatttttg atattcatac gggagggatt gagcatattg
      781 gggtacatca tactaatgaa attgctcaag gatacggagc ttttggtcac caaactgcca
      841 actattggat gcataatgct tggctgactc ttaaaaaggg aaaaatgagt aagagtttgg
      901 gaaatggata tactgtgcaa caattacaag aaatgaatta tgatccactg gcctatagat
      961 atctggtatt aagttctcac tatagaaagg gtttagtttt ttctttagaa tcattggcta
     1021 ctgctcaaaa ggcacttgag aagttaagag aaataacagt cgttgacgga aagggaaaga
     1081 tagacaaaga gtggaagaaa aaatttattg aaaaaattag tgatgatctg gccatgcctg
     1141 aggctatcgc ggtagtttgg ggattagtta aatctaatat taaggcagaa gataaaaaag
     1201 caactttatt agattttgat aaagttctag gtttaaattt agataaaaag aatgttgagg
     1261 aagagattcc gcaggaaatt agggatttgg tgaaaaaaag ggtggaggcg aaattaaata
     1321 aggactgggc ggaagctgat agactgaggg aagagattaa aagagaagga tatttagtgg
     1381 aagatacaaa agatgattgt aaaattaaaa gaatcatgtt aagctaaact tcgatgaaag
     1441 acagtaaata caagtacgag gtgattgtgg tcctgaatcc caaagccgag tcaaaagaaa
     1501 aagagaaaac tattaaaatg gttgaagata aaatttcagg tttggatttt gaagttgaaa
     1561 agcgcgaaga tatggggagt aaaactctta attaccaaat aaaaggtcag gaaaaaggtg
     1621 atttttgggt tttttacatt gggggaaata aaccaataaa atccaaagaa tttaaactgt
     1681 ttttaaatcg ggaaacgaat attattcgtt atttaatatt aaaaatttaa agtagaagag
     1741 gaataaaaat gcctagtcga tgtctaaata aggtcatgtt aattggtaat ctaactcgtg
     1801 atccagagct gcgctacact ccggcgggaa tggcggtggt ctcttttggt ctagccacca
     1861 atcgggtttg gattacaaaa cagggtgaaa aaaaagaaga tgctcagttt caccgaattg
     1921 tagcgtggaa taaacttgcg gagctttgtt ctcagctcct agccaagggc agaagaattt
     1981 atgttgaagg gaggattcaa tatcgagaaa ttactgatgc cgctaacatg agaaaacaag
     2041 tttctgaaat tgtaattgag gatatgatta tcctcgacaa taaaggagct ccgatgcaat
     2101 caacttctca tactaaccct gacgaaacta atgttactga agaatccatg gatttaaata
     2161 atggagccat tgaagatatc gtaatccctg atgatattgg tggtggcgaa gaagtaccca
     2221 ttgaagaaac aaaatgaaaa aattacaaag aaaagatcga aatcgattaa ttagactaaa
     2281 gggtaggggc aaggattgct ttttttgtaa gagtaaaaaa gatcctaact ggcgtgattt
     2341 tgaaaaattg agagagtttt taactcctcg atgtcgaatt atctcagctc aaacaagtgg
     2401 agtctgtatg aaacatcaaa ggaagttgtc acgggcgata aaagaagcta gagagctagc
     2461 cttattgcct tacactgctt ccgaagagta agatagaagc atgataaaag gaaaactgtg
     2521 gcgacagaaa attgaaaagc tatctgattt gacttatgaa gccaaggata aagtgattaa
     2581 ggttgtcaag cttgacgaag ggatattaac aaaagcagaa gtaatcgagg ttaagggcat
     2641 aatctgcctg gaaattgatg attatctcga aagcgaaatt ccaatcaaaa aaataaacaa
     2701 agacgagtgg gataagttga ggtaaaataa aataatgcaa aaacagataa gaaatatatt
     2761 tttattttta gccttatttt cggtagtatc tcttgttagt tttaaggtgg ggaaaactgt
     2821 gggacttgaa gaggcaggca gcaatcaaaa aactgaagcc ttggatcttt cgctgatgtg
     2881 gaaggtaaaa gaaaaactag aggaggaatt tttagaaaaa gataagctta aagacgagga
     2941 aatgacttat ggagcaatct cgggaatggt ctcggctctg ggtgatccat atacagtttt
     3001 tttagctcca aaacaaaata aaagttcaaa tgctgatctt gccggtgaat ttgggggagt
     3061 aggaatccaa cttggataca aagaaaaaag cttagccgtg atggcgccac tagccaagac
     3121 cccagcagat agggcgggaa ttaaggctgg agatttaatt ttaaaaatta ttgataaaga
     3181 aaataatgtt gatagagata cagccgggat tactcttgag gaggcagttg aacttattag
     3241 gggaaaagtg gggactgagg tcactttgaa attgtatcgg gagggagaaa aagatacttt
     3301 tgaagtaaaa ttaataagag ataatattgt ggttcccagt atggaaacgg aatggataaa
     3361 aaaagagggg aaaagtgtaa tttgggtgaa attgtataaa tttagtgaac agatatacaa
     3421 tgactggcct aaggcagtgg aaaatattat aagtgaaaaa aagaagttag ggaacgatta
     3481 tgggggtata attcttgatt taagaaataa cccgggcggt tatttgcagg ccagtgtcct
     3541 ggtcgcctct gattttttaa aagatggagt agtggtaacc caaaagtcag cggacggaaa
     3601 agaagagaaa tatatggttg atactagtcg gggaaatcta ttgaatgata agctggttgt
     3661 tttaataaat gaaggctcgg cctcagcggc agaaatatta gccggggcaa ttaagggcta
     3721 tggtagaggg aaactggttg ggatgaaaag cttcggtaag gggacagttc aatcaccggt
     3781 tgattttgcc gatggatcgg gtttacatgt aactattgcc aaatggttat tgcctgatgg
     3841 aaaaaatatt catggtgaag gggttcttcc cgatgttgag gaaaagtgga atatcaacaa
     3901 taaggacaaa gaagataatc agcttgataa agctgtattg gaaattctga aataaagtta
     3961 tataatttta cgatgaaaag taacagtttt agaactttac taattggagt cagcataggg
     4021 attgttttga tggggtcatt tagtatggga agatggtcag tcaaaagaga tggaagtgga
     4081 ggtcaaattc aacaaaatca aacgattctg gatgaagaat cggtggtgac taaggtcgtt
     4141 gaagcggcca ctccgagcgt cgtcacagta tcgattagta aaacccaggt aataaattct
     4201 tttccttttg gttttgatat ttttggcgat ttttttaata ttccaaaaca aaatccagag
     4261 actcaactaa ttgagcagga tatcggaaca ggtttcatta tcagtcaaga cggactcata
     4321 gtaaccaata aacacgttgt gtttgatact gaggcaaaat ataaggtaat cattggtaaa
     4381 gaggaagaag ttgaggtaca aaatatttac cgtgatccgg tgaatgattt ggccattctt
     4441 aaaattaata aaaccggttt aactccggta aaactaggag attcagataa attaaaggta
     4501 ggtcaaaccg tgattgccat tggcacggct ctcggagaat ttcgttcgac agtgaccaag
     4561 ggggtaattt caggtctagg gagggggata gtcgcttcga gtggactact ggggtcggag
     4621 aagttggata atgttattca aactgatgcg gccataaatc cgggaaattc cggaggtccc
     4681 ttatttgatt cggctggtca ggtaattgga gtaaatgtgg cggtatcaca ggcggctcag
     4741 agtattggtt ttgcgctgcc gataaatatt gtcaaagagt caattgatat ttttaatagt
     4801 accggacaat tcgaaagacc ttatcttggc gtaacttatg ccatgattac taaggaatcg
     4861 gctcttttaa atgaagtgcc agcgggagtt tatgttcagg gaatagtggc tggaagtccg
     4921 gcccagaaag ctggaattaa ggtgggagat attatttttg agattgatgg aaaaaatatt
     4981 gttaaagaga atatagttct ggccactcat gttagtaaaa agaaaatagg ggagagtgta
     5041 aatttaaaga tttggagaga caaaaaagaa atggaagtta aggttgtttt ggagaagaag
     5101 gattagtcag aggttgatcg aggactgtta attcgagaat gagatcataa agatcgccat
     5161 tgaggcattt agttaaatta aaattttttt cgaggcggtg atccttgatt tggtttcggg
     5221 cgtagttata ggttcgaatt ttatctgccc gcatagctag cccgactttt tctctggtag
     5281 tgcccagttt ttcagttttt ttatcttctt cgagctgcca taattttgct ttaagtaaat
     5341 ccatagcaat agctcggttt tgctcttgag agcgttcctg gcgaacggaa aaggttaaac
     5401 cagtcggttt atggaggatt cgaacagcgg tagaaacttt gttgacgttt tgaccaccat
     5461 tgccaccggc ccgggaggcg gtcatttcaa tatcatccgg attgataaca atatcactag
     5521 tagttatttg aggataaacg gcgacggcag cagtggaggt ttggattcga ccatgttttt
     5581 cggtgatggg aatacgctga acccggtgag tgccggtttc ccatttaagc tgattaaaaa
     5641 cgtcagaacc acttattttt aaaatatcat tgtcgagata attgactttc cagccgattt
     5701 tttcacagta gcgaatatac atcctcatta gatcacccat ccacagagcg gattcttgac
     5761 cgcccggacc tggcctgaat tcgagaatag caatattggg attaatggtt ggcataatct
     5821 gaaaaaaatt ctaaattcta aaatctaatt agactttttt t
//
LOCUS       DHGB01000040           10779 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_65235, whole genome shotgun sequence.
ACCESSION   DHGB01000040 DHGB01000000
VERSION     DHGB01000040.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_65235"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    7782..7795
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3086 a         2617 c         1473 g         3587 t
ORIGIN      
        1 ttcggcgggg ctatttttgg ccactccaat aatttttact ctgtctgttt ccttaggtac
       61 ccctaaaatc gtgtacacta ccgcaaaaat aatcaccgcc aacaccaagt tcatcattac
      121 ccctccgaga actatcagca ttttctgcca gggatttttt gaaacgaaag aatctttttt
      181 tattgttgcc cccttttcat taatatcttc accaaaaagt ttacaaaaac ctcctattgg
      241 aagccagttc aaactccaca ctgtccccca cttctttttt ccaacaattc gtggaggcag
      301 tcccagccca aattcctcaa ctcttactcc ggtaatttta gccgccaaaa aatgaccgag
      361 ttcgtggact aaaacaagta tcgacaaagc taaaacgaaa attacaatat ccatagaatt
      421 taaaattcta ccatagaaac aatctcggct cccctgtcaa ggggagctgg ccgtaggcct
      481 gaggggtttt acgctctaat ctctccctct ttctttttgg ctatttcttc cagttttccc
      541 ataaatatat catttacctt ttgcagttct atttcatctc tcttgaattc atcttcagag
      601 acagtctttt catttttggc gtccattagt ttttttcgaa aatcagcccg ggcatcacga
      661 atcatccccc tggctccttc cagttttctt ccgagaagct ttatataatc ctctctttgc
      721 tcaacagtca gcattggtaa acttagtcga attaaggacc catccaccac tggcatcatc
      781 ccaatattgg ccgcttgaat tccattgctg atttcccgaa ctattgcctg atcccaggct
      841 tcaaaaacta aagatctaac atcaggagca gaaattgacc ccaattcctg aagtctcatt
      901 ttttgtccac cataaaccgt cactaccacg ttttcaatta aacctggagt tgcccgacca
      961 gttcgaatcg aagcaatatc atttatgaaa atatccacaa ttctccccat tcgggtattt
     1021 acctcagtaa aatttatcat aggtaattat aacaaaattt taagtgggtt tcggctagcg
     1081 aaacccctac aatatcatat gtaggggcgc cgctcgccgg cgccctaaac aaaaattatg
     1141 ttgcccattt tcttaaattt caaacctctc cattcttccc actttaatat tttccccaaa
     1201 tttcgcattt agagctgtta ccaaatcttt tatttttaaa gaaggatctt tgataaattc
     1261 ctgttcaagc agctccttaa catctttagg attcatcgcc gccacctgta aacatagatc
     1321 tttggctaca ctcaaaaaat cttcattttt ggccacaaaa tctgtttcac acaaaagctc
     1381 gactattact ccaattttcc ctgtactatg ggtataagta gctactattc cctgatgtgt
     1441 ttccctttcc gctctttttt ccgccttctc aaaacccctg gctttcaaaa tttccttggc
     1501 ccttttttca tctcctcccg cctcctccaa agccgcctta atttccataa tcccgagtcc
     1561 tgtttcaact cgaatttttt ttaccagttc tataattttt ttattttcca tttttaaatt
     1621 aattattaaa tcttcaatct tcagnnactc cagccgcctt aatcgctcga ccaatttcct
     1681 ccacaataat actaatactt ttaacattgt catcgttagc cgtaatggca aaatcaactt
     1741 tttccgggtt gctatcagta tcgacaatcg aaactacttt tatccctttt tcccgggatt
     1801 ctttgatcgc tgttttttca aaaccggcat caaccacaaa gataatgtcg aaaagtttat
     1861 caagcttcgt taaacctcca atcattcgtt ccaaacgaat tttttcttta ttaagttctg
     1921 ataaatcttt tttgcttgtt tcagtatatt ttccactttc aagaccactg ttaatatcat
     1981 tgagcttttt aatatttttc ttaatctcgc tccaatttga tactgttcca cctagccatc
     2041 tgtccgtaat gtaaggcact ccagcatcca aagccaccct tcgcactact tctcgagcct
     2101 gccttttagt tccgagcatc acaatttgtt ttccgtttct tttggcatta tagataaaat
     2161 tgcaagccct ttctagacct tcaaaagttt tattcagatc aaaaagttga atcccttctc
     2221 gagcttcata taaatactct ttaatttttg gattagtctt cgaaatttta tgacctaaat
     2281 gacatccggc catcaaaaga tctttaactt cgattgttaa agaatatttc ttatcttcct
     2341 ttggcacgac ggagctttta ttaaaagcgg agtcggctaa ttttggttca ctctgagctt
     2401 gtcgaagggc tttttctgac ttaatttttt cgttagcttc ttttaagcta cttttatcaa
     2461 ctgctttggc ttttttagcc ccaactacta gcgctttttt aaccaaactc tttcccgttc
     2521 tgtttactgt ttttgttttc ataatagtct ttattatctc atttgcccta aatttaaggg
     2581 aaaactattt ttaataaatt aaatttttaa tctagaaatt ataacatatc tattctactt
     2641 gacaataatg ttataaactt ataacattat ctagttagaa atatataaca gtgaaaaata
     2701 aaattaaaga ataccgatcc atccatgaca taacccaagc cgagcttgcg gaggcggttg
     2761 gtgttcgccg ggaaacaatt gtctatcttg agcagggaaa atatatgccc tcactaaaat
     2821 tagcccatga tgtcgctaaa aaattaaaga ccaagattga taaacttttt atcttcaaca
     2881 aacaaaaata aagctaagac tcatttaaag ccccttatat caattttttt aaaaataacg
     2941 tatactggtg agtcctttta gtattttgtc agtcaaaaat gaataagaaa ctctatgtcg
     3001 gtggcttgtc ttttcaaacc actgatgaca ctttaggcgc atctttcgcc caggctggga
     3061 ctgttgtctc tgctgttatt atcaaagata aattctccgg ccagtcaaaa ggctttggtt
     3121 tcgtggaaat gtccaccgaa gaagaagctc aagctgccat caccctttgg gatggtaaag
     3181 agcttgatgg tcgcactgtc aaagtcaacg aggcgagacc tatggaagat cgtcccccac
     3241 gacgcgataa ctattctcga aatcaatatt aattattaat ttccgaataa atttaaaccc
     3301 gccaaacagg cgggttttta ttttttactt catccagctc ctcccccccc cccgccacca
     3361 ccacctccac caccacctga aactccaccg ctacttcccg ctcccgatgc cccgtaactc
     3421 gaggatacag cactagtgac actatccatg gcttctccta ttgatgccaa actgcttcca
     3481 aaatcactcc ccacccctcc ccaataggct ccaattatag cctgatcctg agaatttaaa
     3541 ttaatcggca gttctgataa agcttttatt gagactccaa gcactgtccc ataaactaaa
     3601 tacttttccc aaataatgac cgaatctatc ggctctttaa ctgtttgccc atattcctcc
     3661 aaatgttttt taaatcccag ccactttgcc gcctcctccg cccccttttc cgttctcttt
     3721 tgaatcgaag attccattac aattcccaaa accaaaatca ccaaagccaa aaatatgttt
     3781 aaaataaccc caaagatagt aataatccca agcggtgaca acggcccaat taaaattaat
     3841 cccagcataa aaattataat cgacacccag tttatatatt tccccttcat tcccgccgct
     3901 tctttatcaa aatagttttc ggcgatattt tcggctaatg tcgtcttggg aaatacttct
     3961 gtaaaaaatc ttcgagctcg actctgattt atcctaaacc agctactaat ttcatccaga
     4021 gctatttttt cttcactccc ccgaatctca tcgagcagat ctattaccaa ttgttgaatc
     4081 gaagaggctt ccttttgatt atctttaatt ggctctaaat aatatttata tttcttaaaa
     4141 ataaaccctt ctttttgatt gcttcggcta attttataaa ttttatcctt aattaaggat
     4201 aaaatagtgg cggtcagcga ttttggcgtc aaagcctccc gggcacttat caactgctcc
     4261 acctgagccg ggtcaatttc acttggtggc tcccaccatc taccggagag tgatgcctct
     4321 ggcaactttt tatcctgact atatttttta aatagcttaa tttttcccac taactttgcc
     4381 acttgccatc ctgccagcag caaaaataaa atccccatcc aagcattttt aaataaatta
     4441 aacttaatct ttttttgggt ctcagatata aatttctctt cctccaccac aatctcagct
     4501 tttgttttcg ttcccagcgt ccccccatta aaagtatctt tgggtaaaat aattctcgct
     4561 tcaaaaaaag tttttatagg taattttgat acttccagct ttactttttc actggtcgga
     4621 attgaaactt tcccctcaat aggcccgtgc gcccaagctt gaatttggct tccatcaatt
     4681 ccctcaggca aaataaaatc aatttcaacc tgctcctgca ttatttccca atcaccgcct
     4741 attgcctgcc agtaaatctg cgccacgtcc cgctctaaag taaccgcatt attaactaaa
     4801 taacttaaaa taaaatcttt actctcactg ttagctttat aaaaccattt cacatagtag
     4861 cggctcccct catccttaac ataaaaagtc cttggtactt ttgccacatc atctggagca
     4921 atttgctcat aacacccggc agcttcacaa atttttatct cttttaatat atagggctcg
     4981 tttctctccc cttttttatt aatatattga taagcaaaag tgaagtcgcc gctaaaatta
     5041 aaagttcttc tttcttcaac cagcattgac ccgtcccgtc gcacctcggc tctaatattt
     5101 accggtgcta tggtatatga tttagcctga gctttattta ctcccaaaaa cattgccaaa
     5161 atcaacaaaa tcaaagacat aatttatttt acacccccct ccctattatt cttttttctt
     5221 tcgatttaga tacaaagaaa agcacagtaa gccattaaaa aagaaatagt cgattcggct
     5281 ccctgattca gatttacttt attaatatca ctaacactat caaaacaagc cccgtttttg
     5341 tcaaccatga ccactccctg agtattattc ccccagtacc agctccacca gcgtcccgct
     5401 tcttgtaaat aataaatatt atttgtcgct aaataagcct cttttaagca gcaaaccatg
     5461 taagcgacat caacactttg ctgatcatat ttattttttg ctcctccttt gtaataccac
     5521 cccttacagc caatcggcat tggtattttt ccttcttgac aagtctcgag taaaaaatca
     5581 gtcgcctctt tggccacttg aattgactcg ccatcctctc taatccttcc ccttcgccac
     5641 aaagcccatg gcaaaagagc gttagcatag gttaaaaaat cctcaaacca agaccagcct
     5701 ttttcttttt taaaagcatt agttaatttt ttcgtcaatt ttttttccag ttcagactcc
     5761 ctttcttcac taactaaacc gagcagtaaa taggccatcg cccgcggcga gagccaatca
     5821 tcaacctcct ctaagattaa atccgcaatt tttttttctg tttttgtctc catcagcacc
     5881 caaaccacca ttccgagggc atcttcacta cccttaatct ttttatccga aacaatttca
     5941 tcgttattgt cataataaaa ctgccaaata ccttctcgac tcaccgccct tcttataaaa
     6001 ttaagataaa tattagctag ttttttttct tcaaagcaat ttgccactaa gtaggctcta
     6061 gcctgatcat ccagagcata accaaacttt ggatccacct tgtcatatag gccatgttga
     6121 tatattcccc atttcgttgt catctgatgc aaacgtccca gccattcatt ttttatgttt
     6181 tttgacatat ttctttattt ctttttggcc atcagctcaa acatatctaa atgttgtaga
     6241 gctacatttg tccaggtcat aaagcggcca tattcataag cctttttttg tatttttttc
     6301 attttttctt tgtccttcca tatttttaaa accgacgttg caattgcttc tgaatcccta
     6361 aacggcacaa ttaccccctt ccctttggct aaattttcct tggcatattt atagggagtt
     6421 gaaatacata tctttccggc cccgatagcg taggctaaag cccccgaaga cgactgctcg
     6481 ggatcaagat agggagttac ataaaaatca agtgtcttaa gccacaaaat taaatcatcg
     6541 agtgataaat atttatcaat aaattttata ttattttcca ttcccagttc cttaaccttt
     6601 tcttttaaga aattacgata cttttctcct tcagactcaa tcactccagg atgagtctgg
     6661 ccaataatta ctcccattat ctcggggtag actttaataa ttttagctac tgcctcgata
     6721 gtgtattcca aacctttatt ctcagaaatt aaattgatac ttccaaggac tattttaccg
     6781 gtaagccctt tttgtttttt ataattctct gttgagctta aaggaatgtc aggggttcca
     6841 tggggaataa aagctatttt ctttttgtca acgctatagc tttttatcaa ttttttctga
     6901 ctatccttgg tcattaccgt aatcccacag actattttcc ctagttcttt taaaacttta
     6961 ccataacccg tctccgcttc tttaggaata gtgtggcaag tcattattaa aggctttttt
     7021 atcattttgg caaaatcaac ggcgaaagca ccaaaattac cgccaaatat tccaaactca
     7081 tgttcaatta aaaccacatc gcagccggaa ttatttatat aatttgccgc ctgtttataa
     7141 ctttctaaat tattccgctg aattttatat ttaacttccc gaggaaattc taaattctcc
     7201 tcgccctctt taactattgc cacaatctct gccagagcat aaggattaag catattaatg
     7261 gccgtagtta catctttagt gaaagtggca atgccacatt tttgcgggat ataagtcgat
     7321 acataaatta cccgcactgc ctttcttttt tttgttaaat ttaagatctt atccctcatt
     7381 tattaaaaat ttcagtaact ccgacaaaga catggttact aaccctattc ttaggtcagc
     7441 cgcaccgtaa tatatataaa gcatatcatc ttcgactacc gcccccgttg gaaagaccac
     7501 attatccacc attccttctt tttcccagct cttttttggt tcaaataagg gatatggtaa
     7561 tctttttaat acttttcggg gatctttttt gtcaagcagg gctatacttg cccggtaaat
     7621 tttattatta tttttgattt ctactgaatg ataaattagt agccagccca attcagtttc
     7681 aatcggaaca cagcccgccc ccacataagc actttcaaaa cctcccaccg gatctaatac
     7741 gatatgttta tcaagctcag ctaaatattt tttccagtaa tnnnnnnnnn nnnnncattg
     7801 ctatttgacc attaattctt tgaggaaaaa gcaccacatc tttttcccaa agtaatacat
     7861 tgtcgccatt aatacttcga taaacttttt caaaaaaatt atatcttgga ttaagtttcg
     7921 ttttaagtct aaaaatatct tcagcctcat catagctaat tttaggaatt attactcctt
     7981 cctttctaaa atgaaccaga tcggttgaag tggccatcgc cacggtcgca tttttaccat
     8041 cataagccgt atatagcaaa taatatttgt catccaaaaa agttatccga ggatcttcca
     8101 ctcctctttt ttcatattca aattcgggaa atataactgg tccaatactc tggaaaatta
     8161 cttttccttt ttttaagtga cagcaaccga tacttgacac attttcttta ttcactgccc
     8221 gataaaacat atatgtcacc ccatcaacta aaatacaacc tggattcaaa accccattag
     8281 cttcaaaatc tatcccccgt cttttctcca gcactatgcc cttataattt atttttatca
     8341 ttactatttt ccaaaatatc taatcaaatc atgtttcaaa ttatacaata ttatccctcc
     8401 ttttcccctt ctctgtttcc atgctagtat acatactatg actcaaacta cagacctctg
     8461 tcccaaatgc ggtgcccccc tatccgaaat aattaaaact ccctctggcc gtgagctcca
     8521 gcgttgctct aaaggatctt ggaatcctca gactcataaa aatgaaggct gtgattatgt
     8581 taaatggctc gaagttaaac cgattgaact cgatgaaaaa tgtcctaaat gcggttctca
     8641 tctgatcctt caagtcaccc gttttggcaa gaaaatgaaa aagtgttccg ctagctcctg
     8701 ggacaaagaa aagaaagttg ctattggctg tgattatatt gaatggatta acggaactag
     8761 cgaaaatcta gatgaaaaat gccctaaatg cggtgaaaac ttagttttat acactaccgc
     8821 cagcgggaag aggatgaaaa aatgctccac ttctggctgg gataaagaag ctaaaaaagc
     8881 taccggctgt gactatgttc tctggctcaa agctggcgaa aaagatagcc aaaatggaga
     8941 agaatttctt cccccagaag aaaactaatt atttttttac cagcactatt ccaaaaggag
     9001 tttgtcgtcc tggtccagct acatattttc cgtttaccca aaatgccgtc gagcccccac
     9061 tatccaaatt caaagcccct tctattccta aagtctttaa cactttagct acctctgcca
     9121 ctgtcgcatt tcgcaccacc ccgatataga ccagatttcc cttataagca ataaaagatc
     9181 ggctcccttt ttgagttctt ttagtatcat tgtcgtttcc atatctaatt tctccgccgg
     9241 ataacaacag tggttgactg gcaatcacac tgtcaactcc agtatcccta ccccaatctg
     9301 agcttttccc caccagtctg gcactatttc cactaaaaat caaagctggt acactactgt
     9361 aaacattatt tgaggaatta aaataatatt tatttttatt catgaggagt gtatcaaaag
     9421 aatttgtttt tccactacag ctcggatatt cagccggaca aaaatagggt ccattaatcc
     9481 cggcaaagcc accgcctctg ctgacaaaat cagctaaagt agacactggg caattattga
     9541 cacagtcact ctccgaggac gtctccacaa tcacttttgt tgaatcaaga ctggccgcca
     9601 aaatatcaat tacaaaggtc tctccatcca cctctactcc ttgtcttgaa taaccgctgt
     9661 caggagtttc gttagatttt tttacctcca aaactgccac tgccgattta gaagcaagag
     9721 tcgcccgaag tgaattaatt tctttttcaa gtattaataa attatcatta gcactggcat
     9781 aattaccttt tgataaaaat gctaaaattt gactaaattt gctatatatt ttttgatttt
     9841 tactaatatc aactcttaaa tcaattaaat cttcataact cttcaccgcc tcatcataag
     9901 ttttttgaat attttttatc tccccctcca aggccaaatt ttttttatac tggtcatcat
     9961 tttttaaaga ttcgatctct tgttttttaa gatttagctc tgaggaaata tcatttattt
    10021 gattaccaaa ttgaaattcc aaactgctcg tctctctttt atataaaacg taaatccaaa
    10081 tcgagattcc caaaaacagt cccgccacta gcatccaaag attaattttt tgtttaaaaa
    10141 acttcacctt ttaagtgtag cattatccca cagtctctat cgcctgcttc ttctcataaa
    10201 tttcttgtat tcctctccac ctcttgccaa gagcccctga tgtaaactca aagccttaaa
    10261 actgtcatct aattctctta agctacccgc gccataaatt aagcccttac cgccgccgtt
    10321 tggaaacata attaccccct gcccaccctt cggtactcga atcattgaag attttccata
    10381 gcgcctatcc ttgactagtg cattccagcc attggccaca aaaattgtct ccagcgttgt
    10441 caaggctccg gggatagtac tattacctcc agttacgaca tcattacgaa catttgattg
    10501 attatccatt tgaatcaaac ctaaataagg atctttcctt gaaccatagt taccgactcc
    10561 atcaacaact aaaaaattaa agcggttatg accaatatca aaggttactg tcgctcctcc
    10621 ttcgtacaaa ggtgtccctt taagtttact tttataatac aaataatctc cagttattag
    10681 tgctttgcct ttccatgacc ctatatcgag taagtcttct tcgtcacctt caggcatatc
    10741 ttcattctct tcatctagcc aatctttttc gtcaccttc
//
LOCUS       DHGB01000041            3126 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_121360, whole genome shotgun sequence.
ACCESSION   DHGB01000041 DHGB01000000
VERSION     DHGB01000041.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_121360"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT          931 a          605 c          564 g         1024 t
ORIGIN      
        1 ctaaagaacg tgattattgc tactcttttg agaatattcc aaaagtgatt attttggaga
       61 gatggccaat aagcacagca attctatctt atttaagaca gaaattcctt agcgtacgat
      121 ttttaccgaa ttctgcgatg accccttatt aaacaaaatg caattaaaaa gcaccggcag
      181 gggagggcgg cccgggggcg ctgcccccga atcccctgca aggtggctcc catgccccct
      241 ggattctgcg attttccctg gccttcttac ccatatcccg ctggagatcg tgccccctgg
      301 ggcatcctga gaatatttcc ccgaccccaa ccaggtccct tgttaaaaag gtctccactc
      361 gggccccagg tcccaggctc cggctcctca atcacccggc ggggcagggg aggcgcaggc
      421 tacacttaac cggcgcagtc cctggatgcc atcccggcct ccggccgcga ttatttttcc
      481 cctcgctccc tcaatgctgg ctcggtcgct tcaagggaaa ataccgcatt cgccggggat
      541 tccctgcgtc ggcccaagtg gctgcccgcg cttcttttta gatcgagtct gccgcagagg
      601 ggtgattgac tttttctttc attgcctact gtctttatca gcatttttga ttataattaa
      661 gttatggatc aaaaaagttt cgtaaaaata aaaaaaatca ctctgaaggc aatgtactca
      721 gatgatgatt tgatggaaac tcttgtattg aaaggaggaa atgcgattga tctaatatat
      781 ggggtttctt ccagaagctc attagattta gatttttcca tgcaatcaga ttttcaagat
      841 attgctgttt ctgaaagtaa gataaaacaa actttagata aaactttcaa tggagaagga
      901 tatcaaataa ttgattttaa gtttaaaaag aaacctcaaa ttagtgggga agataaaatg
      961 aaattttggg gaggttattc aatagatttt aaagttattg ataaacaaat atttaatgac
     1021 aatcagggtg attattcaaa attacgttta ctcgccagtg atattgattc gaaacacaaa
     1081 aaagtcttca caattgatat tagtaaatat gaatattgta caggcaagga atttagattt
     1141 gttgatggcc ttaaaatata tatgtattct cctgaaatga ttattttcga gaaaataaga
     1201 gctatttgtc agcaaacaaa agaatacctg aagataatgg aggctcaagt gccaacccct
     1261 cgagcccacg atttttttga tatttatact ttaatgaaga agcttaaaat taaaacgaat
     1321 tctttggatg taagtattct aaaaagtatt tttgaaataa aaaaagttcc ctttgaactt
     1381 ataaataaaa ttgaagattc cagagaattt cataggctcg gattcgatca ggttaaagat
     1441 actttgaagc caggagaaga attacataaa tttgattttt attttgatta tgttttagat
     1501 tttattcaaa agattactta aacccttttg gataaaaaag tttccattta ggagaatact
     1561 ctttttctat catgttataa gtcaaataaa aattatattt gatttcaaat ttgtttagca
     1621 gttcgatttg agattcttta tacccagcag tttcaagata aaatccaatc acttgatgat
     1681 agggataaat ataattaatt ttttctaaaa gcgcagttag tttatttatt gaaacgtttt
     1741 tttccttagc taatttatat gcatttaaaa cttggcttac ccctccagaa taaaaaggtc
     1801 gcactgaaat atcaattaaa gtcctttcaa tattcgttac tggaatcatt tcatttaatt
     1861 gaaaatcttc tactcccaat cgtccagtgt ttttcccatt gagcagatat attttatgat
     1921 cttcaaagat accgctattg ttagtcatcc tcggtttttg ggaaaatgcg cgatcaatat
     1981 ttttttgaat taattcaccg ctttgcatag attttgcagg ctgctcaaga tttatataaa
     2041 tacttttcgg gatttgttct gttaagttat gaaaaaacat agctgaataa tgagaaagat
     2101 aagcggagcg atttaaggag gctgcgaggt ttaatattga gtattctccc caagaatatc
     2161 taatatagtc ttttgaagga aattttatct caattttttt taaaggagtt ttatttaaca
     2221 tggcatttat gaattcgtaa tatgaccagg tactacccaa tcgccattca gctctattat
     2281 tacagagtat ttcagatagt tttgtgtcag aaaatatatt tatgcctaaa cgattgaagt
     2341 gatcaatgat ttcttgctta gctaaataga atttagttat atttaaaact ggcattttat
     2401 tacagcaaga tgtatatata catcttgctg ctccatataa aaacattata atatacttaa
     2461 gatcattttg caacagttat tttaaggttt tatcatgtat ggttgcatat ggtatatata
     2521 ccatatgcaa ccgattatat ggtttttcaa caaaatacca aaattcatgc agtccaaacc
     2581 tgggtaaaag ctttccagtt ttaaaaaccc tttcaaagac gtgatatcgt ctctgcagat
     2641 cccgatcgat cgtaagccgg gatataccta aanntgatgg cagccgccag cggccacttt
     2701 accattaaat cctctactcc tttcgggcct tcttcagatc gcgcctcaga atcgatcctg
     2761 gtgcgtcctg tatcccttgc tggccctgca ggaccattcc tggtcatttc tcagctgccg
     2821 gctcgcccga aacagcgccg gcatttccac cagggccttt tccctggttt ctgccttagc
     2881 ccgtttgaga tcgtcgattg tagggcgtcc tagcacgttt tttcctgctc catttttgtg
     2941 caatcagggt tcatcctttt cccatatgcc tttccgagtt ttcccgatcg atttttccac
     3001 tatttatctt aattacatca ttaagttttt attatacctg acggagccag ccaactcccc
     3061 cagattgaaa gacaatttta tggccaagat tactctggtc aacgacaagt tagggtatac
     3121 tctaat
//
LOCUS       DHGB01000042            3448 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_239220, whole genome shotgun sequence.
ACCESSION   DHGB01000042 DHGB01000000
VERSION     DHGB01000042.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_239220"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    798..843
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1151 a          517 c          680 g         1054 t
ORIGIN      
        1 aatgtattga caaatttcta cgttttggtg taaaatattt caatcatgag agagaatacg
       61 aactaaggac tacgtgtctt tgtgtgacgc ctctcatttt ttttgttttc ttttctttct
      121 ttttgtttga aattgcgttg gtctatattc taatttactt ttttctgatt gaataaaaac
      181 tttaaactct tgaaaaggtt ttaacaaaga tgactctgat ttttgatttg gaattacaac
      241 tttaaataat ttattattat tttttaaaaa ttcgagaaga catctataat caccatctcc
      301 agccacaatt actgctttgt caaagttctg atattgtata gcagcacagt taagaaccaa
      361 ttctgaatca acatttcctt tcaattttcc attattgtct tttacggttg gtttaaaaat
      421 taattcataa ccatatgatt ctaaatgctt atatagttcc ttatactcag gtgaaaaacc
      481 tataaaaata tatgcctttt gaactctgaa tttatcaaaa agatattttc gaaatttttt
      541 aaaatctaat ctccatccag aatatattaa aatttttcct ttatataaat tttctgaagt
      601 acctaggttt aaattttgac tatcaatgaa tgcaaatact ttttctgtat ccatatttct
      661 attctactgc cattatttga ttttggtaaa tttattacat tgtcagattt actacttttt
      721 ttagtgaata aaatagttta aagttttgta taaatgctta caaagatatg gagcgcggca
      781 aattatggtc ttgaggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnntagttaa ttcagaaatg gaattcccaa cggctaaaat tacggtaaat ttggccccgg
      901 cggatctgcc taaggaaggc gcctgttatg atttacccat tgctgtaggg atattagcat
      961 ctagtgagca aatcatttta cctaaggaaa aagcttattt ttttggagaa ttatccctgg
     1021 atggaagcct gcgccactct agaggagttt ttcttttagc aattttggcg aaggaaaaaa
     1081 gagtaaggaa aatttttgtg ccaagactat gtgccaacga agcggtagta gttgaaggaa
     1141 ttgaagttta cccagttgat aatttgagga gtttaatcaa acatttgagg ggggaaaaat
     1201 taattaaagc tttaaattat gttgaaagta aggatttaat tgatgaaacc ttggttcctt
     1261 ttgatttttc tgaaatactc ggtcaggaat cagccaaaag agccctggaa attgccgccg
     1321 cgggtggtca taatgtgttt ttaatcggtc ctccgggctc gggaaaaacc atgttgtcaa
     1381 aagctcttcc tggcattttg cctcccctat cggaagcaga aagtcttgag gtaacaaaga
     1441 tttattctat aaccggaaac atccctcccg gtggttcact aatcagaaat agacccttta
     1501 ggtcgcctca tcacaccact tcacaggtag gtctaattgg cggtggcagt aacccaaggc
     1561 ctggtgaaat ttctttatcc catcggggag tactgtttat tgatgagttt ccagaagtat
     1621 ccagaatgag tctggaagcg ctgagacaac ctttagaaga tggctatgtt tgtatatcta
     1681 gggcctcggg ctcagtttct tttccctgtc aatttatgct ggtagcagca gcaaatcctt
     1741 gcccgtgtgg ctacctggga gatgaaaaaa aagaatgtaa atgtaatatt caccaaatta
     1801 tgaattatca aaaaaaactt tctggaccaa ttcttgacag aattgatatt catttaaatg
     1861 tgccttcggt agatgtcaat aaactgatga ttgggagcaa ttcaaactcg gctgaaaagt
     1921 cagaagtgat tagagaaagg gtaattaagg ccagagaaat tcaaaaaaag agatttgaaa
     1981 ataccaaggg aatctatagc aattccgata tgaaaaataa gcatttaaaa gagtgggcaa
     2041 aactggaaga tagcgctaat ttactcttaa aacaggccgt aaataaattt tctctatcgg
     2101 ctcgaactta ttttagatta ataaaagtaa gccgtaccat cgccgattta gcggcctcgg
     2161 aaaacattaa agatattcat atcgcggagg cactacagta ccgagtgagg gtgggagagt
     2221 aaaaaagaaa aaatctaaaa ccagataaaa catatgaaaa cgaaagagga aaattatcat
     2281 aaaggaagat atggcgagaa tattgccaag gagtatttga ttaaaaaagg ctatgtgttt
     2341 atcgaagcaa atttcgaaaa taaaatcgga gaaattgatt tgataatgag cgacggagac
     2401 tggcttgttt ttgttgaggt aaaatataaa agtgatgacc gcctcggaat cccagaagaa
     2461 atgattagtc caaataaaat ttggcaagta aaaagggtag ctgaaagtta tttagtagaa
     2521 agaagaataa aatattttga aaaatttaga ctagacgccg tgtgtatctt aggtaaggag
     2581 attagacatt acgagaacat tggatgaata agtggaagaa gtggccaata aaaattatta
     2641 ggaaaataga ttttccgaaa caactagtag aaataaaaaa ttgtccggat aaattatttt
     2701 accgaggaag ctgggacaat aatattttta ctaaaagttt ggcagtggtc gggagtcggc
     2761 gaatgagccg atacggaaaa gaagtaatcg ccaagtttat gcctgagtta gtcactaata
     2821 aaatatctat tatttccggt tttatgtacg gggtagatac tgagtcacat ttaaaatgct
     2881 tggaattcgg aggaaagacc gtcgctgttt tgggtggcgg ccttgatatt ttaactccac
     2941 cagaaaacga taatttatat tgccaaattc tggaggcggg aggcctcgtt atttcagaat
     3001 ataagccaga ttttcagcca actttatgga gttttcctca aagaaacagg attgtagctg
     3061 gtctctccag tttgggtgtt ttagtcatcg aggcgggcat aaaatctgga tctctaatta
     3121 cagccagaat gggccgagaa caagggaaaa acgtttatgc tatccccggc caaatcaaca
     3181 atagcattgc tgaaggtacg aattacttaa tcaaaaatga tttagcgaaa atgactttat
     3241 cagctgagga aataatcggc agtcataaaa tacttcagga aaattttttt gagagtctta
     3301 atcctttgga aaatgaaata ataaaatttc ttaaaataga atctttgagt gtcgatgaat
     3361 taagcaaaaa attaggtaaa tcggtgatag aaaccgggat aaaaatcgga atgatgagcc
     3421 tccaaaatat tatttttgaa gagagtgg
//
LOCUS       DHGB01000043           18091 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_30195, whole genome shotgun sequence.
ACCESSION   DHGB01000043 DHGB01000000
VERSION     DHGB01000043.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 18091)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18091)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18091
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_30195"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    8354..8374
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14210..14244
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6502 a         2692 c         3703 g         5136 t
ORIGIN      
        1 aggcgaagaa acatgtggtt gacgatgtaa taacaaaaga atatattgac gagtagatat
       61 aaaaataaaa atatttttta aatgaaaaaa aatcccgaga attcattccc aataatttca
      121 attgaatctt ttggaggaac tgagggatta gtaactggat cttgccacag attaagcatt
      181 aagaactcgt tgatactggt tgattacgga ctattccaag gaagagacga aaaatcacac
      241 aatgggggaa taaacagaaa ttctgaaccg gtaaaagata tcgccaatgg agttaccgat
      301 gttttagaaa ctcatgttca tatcgatcat tccggaagat tgccattgat ttttaaaaaa
      361 ggttttaccc caaggatact agccactaaa gaaacagcaa tttttatgga acccatgctt
      421 tataactcgg caaaaattca ggaaaatgag aaatcttctc aaaaacttta tggaactaga
      481 gatgttgata aaaccttaag tcaccttaag ataataaaac cttttgaact aatcccaatt
      541 ggacagaaac atgacagggt aacggcagag tttttactaa acggtcatgt tatgggaagt
      601 agctcaattg tgattaggga acatggaggg aaaaaaaata tactatttac cggagatatg
      661 ggtaaaccaa atcaatcact ttgtggtggt tataccaatt ttgttgatca atatcccaat
      721 gaccctataa atgtaatggt agtagaatca acaagttttg ataaagatcc agtttccttt
      781 gaagaaaaaa gaaagggttt attggctgca attaatgaag tatgggaggg aaagggtaat
      841 ccggttatac ctgcattatc ttttcaccga acgccagaaa taatggaaat gcttcataat
      901 tgtcaggaga atggtgactt acctgaagac tgccaaataa tcattgatgc tcctttggca
      961 atggaattac ttgatacttt taaaaaggaa cttggatcga actgtttatc aaatggctat
     1021 ggagatgatc cagaatttta caaaacaatt gaaaatagca ttaatagatt taacctaaaa
     1081 aacgtaacaa ttatcaaaga acataaggag tcggtagcct ttagcaaaaa agctgcaaac
     1141 agcaaggaga ggctgattat aattgccagt ggaggtatgg gtggtcacgg gaggtcagta
     1201 aattatacta gaggtgaatt tggaaacaat cctcgaaatg ccgtcgttca aacatgtttt
     1261 caagtaccag gaacggaggg ggcgaaatta ttagaaaaag gaagggtgac taatggtaaa
     1321 aataaggggg cgagagtagt aaaaattgag ggatttacta gccatattag tggttcagat
     1381 caaacttttg ggtttttaga aaggtttaat ttgagcaatc ttgaaaaagt aataattact
     1441 cacggtaaag ataaggcaag aataaccatg gcaaatgatt ttaaacgaag ggggtatgga
     1501 gcggaaataa ttttatcaaa aatacatcaa attatagaaa tataaattgg gataatgtgt
     1561 taaacaggtt ataatagata ggtttttgtg taatttaatt cggggattgg cgcaattggc
     1621 tagcgcgcac gcatggggtg cgtgaggttg caggttcgaa tcctgtattc ccgaccagag
     1681 aaatattaac ctcgaaaaag taagaaaaat agaaaagagc gaagatatag gacagccatt
     1741 tggaatctgg gtaagggata aaagggcaga gaccagcgat cataaacatt ttgaagctgt
     1801 aaagaaataa gacgaccgtt atctccacta aaattatcgg ctggtatgga atccgaaaat
     1861 tcgattttaa attttggaag aaaatatttg acgaaaggaa aaattcggag aaaatcccaa
     1921 atggaagtac cactaacata agccataaca attttggttt tttcgggata ctttagattt
     1981 ttaataatat gcccaacacc cggcaaaaaa tcgtggccat tttcagaacc accagccgga
     2041 aaaatagcca ccagagaacc ttcgtcaatt ttttgagagg cccgagcaat gctttttaaa
     2101 tttttttgat gagcaatttc ttttgaatat tcgggaataa aatggatttt tttaaaaaaa
     2161 tgatatttcc aatcgttttt ggctttgtca ttaagacgat gagtgatgta gacagggata
     2221 agatatttgc caattgccgg caaaatagat gaaagtccgt gcatgattac caaaaagatt
     2281 ttggagcggg gaggaacggc agccaaaagc aacaagacat cagcttggga aggatgatta
     2341 caaattagaa gaacggggtc gtttttaaga ctttttttag ttgattttgg tatatttacc
     2401 gcaaagttac gtgatacttt ttctacaata tttttaaggg tgcttgaaag accgtgaact
     2461 tttaagttgt gattgatagg gcgaagaatt tttttgacac caaaagtggt atagagtgaa
     2521 cgaggcatga gtaccactat aacaaaaatg gagaaaattt tctaatttga ccccttgtat
     2581 attcgggttt tattcgggta tgatgtgaac atgagacaaa aaataaatgc accagtatca
     2641 gtaatgatgg attttaacca ccggcaaaat aaggtcatgc caacacaaat tcaatggaat
     2701 aatcgcgatt ttaaaataga aacagtcggt atgcattatc agttttttaa aggaaaaaag
     2761 ttatttcatg tcttttcagt aagctcgggg gggatgtttt ttaaactggt cctaaacacc
     2821 gaaaacttat tttggaaatt aagcgaagta gccgatgaat tcacctaaaa aaatgctggt
     2881 cgatatcaat tcctgttttg ccaccattga gcagcaggcc aatcccctac tgcagggtaa
     2941 agaggtagtg gtagccgctt ataaaagtga aaggggttgt attttagcgg cctcgagaga
     3001 ggccaaaaaa ctggggataa aaaccggctg gagaataaac gaagcccaaa aaatttgccc
     3061 taatttaatt gtcctaaccc cagatacaga aaaataccgc ttcattaata aaaaattaaa
     3121 aaaaatatta ttaaaatttt gtcctagagt acaaacaaaa tcaattgatg aatttttttt
     3181 agattttgaa ggaataaacg acgacttaat cgaaataggt ctaaaaataa aaaaagaaat
     3241 taataaaact ttaggagagt ggataacagt ctcgattgga attggaccaa atttattttt
     3301 ggctaaaata gcttcgaatt taaaaaaacc agatggcctc gttaaaatag acgaaagtaa
     3361 tttcttagaa atttataaaa aattaaaatt aactgatttg catggcataa accacaaaac
     3421 ggcttataaa ctcgagctaa atgggataaa aaatccggat gatttctatt ataaaagtca
     3481 acaggagctt cgaagtattt ttaaatctat ttgcggagac tactggtatt ttcgcctgag
     3541 agggatagaa gtggacgaca aggagaagcc agttaaaaaa agtttttcgg ctattttttc
     3601 tttaaaaaat ccggctaaaa ataaggctga tttggcagga atattttttc aattgtgcct
     3661 taaagtgggg aaaaggtgca ataaacagaa aatgggaatt cggggcgtgg cctggtgggc
     3721 cggaggagaa aatattaata ttagtggtaa gttacataaa aaagaagaga tgattaatgg
     3781 ctgggaaatc tttaaggaaa tttatcccaa gatacctgaa ttcagaggca agataaaaaa
     3841 agtagtggtg gtaatttatg atttgcaaaa aaaagtttgg cagaaaaatt tttttacaga
     3901 aaaagagaag tttagacaag gaagtgaaat cgccgaaaaa ataaataaca aatacgggat
     3961 ggggacagtt tttccgggaa cagttttggc aagcaaaaaa attcccgatt ttattggctt
     4021 tggcaataca gaataaagat gatattctat taattcaatg gtgaacgaag ttatttttga
     4081 tatcgaaaca aaaaaaattt ttgatgatat tgaaggagat aatccggcgg atctgggaat
     4141 ttcaattgtg tcaatttata aaagaaagct ggatgataat tataaggaac tagaagggaa
     4201 aatagagagt ttttttgaag cagattttgc caatatgtgg gctttatttt caaacgtgga
     4261 ccgaattatc ggttttaatt ccttacattt tgatgttcca gctctagctc cgctagcacc
     4321 ttatgatttt aaaaaactaa aacattttga cattatggac catgttaaaa attctctagg
     4381 atttagactt agtctcaacg ctattgccac tgagactctg ggccatacaa aaattgataa
     4441 tggtctaaat gcggtttatt actggcaaga acatagtaaa gagtctctgg caaaattaaa
     4501 aaaatattgt gaaatggatg tcattgtcac caaggaaatt tacgactacg gacttaaaaa
     4561 taagcaatta aaatacaaag ataaatggaa taccccgcgg gtaatagaag tagatttttc
     4621 ctatcctaag gtagaagtag tgccacaaat gggattattt taaagaattt tactttctat
     4681 ttctttttca gatttattca tcacatacac atcagcttta taatcatcga gaccgacata
     4741 tttggcctgg ccgcgaagaa tggcttcata agaacgacaa gcccggcaac cagacttgcc
     4801 accgggacaa tcaaatttat taagtgattt ttgaagcttt attctcttgg cgatttttaa
     4861 aattttttct tcggcttctt tgagatctgg taattttttg tgggtcaact catcactact
     4921 gtcgagatac caataggaag cagcggtaac ttccctattt tggcaatttt taactaaaag
     4981 atgatagatg ggtagctgaa gagaatcctc tccttcttcg tgacgagagg ttttaaaatc
     5041 gataatttca acactatcac tgtctggata atatttaagc caatcaattt tgccgcataa
     5101 aattatattg tctgtctccg acagccaata ataaggtaaa tccatattga ttttaatggc
     5161 cagttctccg agtgggccgg gattgttgat aacccgacgg atcatagcct cacctctttt
     5221 tttgtattca tattcagtgt ttttatcaaa aaaaccgcct ttttctccag aaaacttttg
     5281 ccaggagcgc tcaaatttat caagcagggg ctcttgaaaa cgaagatcag ttttaatttc
     5341 tgaaagagac tcaataactt cgtgaacagc ttgaccgaga gccaggggtg gcgacataag
     5401 cttgattttg tggccagttt taggatcttt ataaatattt tttaaaaaat aagcccgggg
     5461 acattcgaga aaagtagaaa tagaagaatg agagacccaa agagcagtgt atttatcgga
     5521 cataaaattc attatactat gcaatgcgat taaataaatt tttagcgggg gctggaattg
     5581 cctcgcgaag aggggcggat aagttaatag acctcggaaa ggtagaggta gatggaaaaa
     5641 cggcaaagct aggagataaa gtgcggggaa atgaaaaaat tctggttaat gatcaggaaa
     5701 ttaaatcagc tgaagataag gaatatatta tgctttataa accgatggga atagtgtcaa
     5761 cagccaaaga cgaaagaggc agacaaaaag tagttgacct tgttaaaagt aaaaatagac
     5821 tttatccggt tggaaggctt gatataaact cagagggttt gatattgtta acaaatgatg
     5881 gtgatttggc gctaaaatta actcacccta aatttcattt agaaaaagaa tatgaagtag
     5941 ttctcgacag agaaataaga aataataaaa ttaaacttgg tattaacaaa attgtcagaa
     6001 ttgaaaaaaa taaaataaac ataattatgt ttgaaggtaa aaaaagacaa attagagaaa
     6061 tgtgctggaa ggcgggactg agagtcaaga ttctaaaaag aattaggatt ggaaatttaa
     6121 aaatcggtga tttaaatcct gggcagtcga gacacttaac tgcccaggaa gtatctgaac
     6181 ttaaacaact ttaacctgta tttctcgggg tttggcagtt tcggccacct tggctgagac
     6241 ggcaatgaca ccgtctttaa ccttggcctc gatactttta ctatcaatgg gtcgtggcaa
     6301 ataggtggtg tattcaaaag aagagacttt attccactga tgaacaattc gtttaccctt
     6361 cttttcggtg tctttttctt cacttctagc tgagacacgg agaaccccat cttcgtaggt
     6421 aacttttatt ttgtctgaag gaataccagg tacagctgct tcgacaatta ctttatcacc
     6481 ctcttcatag acattaagac cgctggtcat tttcatttct ggccattcat cttcgtccca
     6541 aagtaggggc ttaaaatcaa ttaatggttt atagggatag tagtgcatag agcctccttt
     6601 aaaattaatt aatttataat tattagcagt ataaaacact ctctgctagc tgtcaagata
     6661 tcaaatgcta atgtggtata ttaatcaaat gaagaaaaag atttgtttgg tcttgggaag
     6721 cgggggttct aaaggactgg cgcatcttgg cgtaataaaa gcactggtag aagccaaatt
     6781 tgagatcact caaattgtag gaacttcggc cggagcaatg ataggtggtc tatatgctgc
     6841 caatccaaat attaaagaga tcgaagacat tgtttatagt ttgtcttatc gtaaattatc
     6901 aaaaattatt ttcgaaagac caggaaaaag tgcattaatt caggggaaaa attatcataa
     6961 ttttattgca aaaatttgtg ctgtaaaaat gatagaggat accaaaatac cttttaaagc
     7021 agtgacctgt gatttgatta gtggtaataa atttgttttt actaggggtc caatagccac
     7081 ggcaatttgt gcttccagtg cgattcctgc aattttttct ccagttgaat atgaagaaaa
     7141 attgctgatt gacggtggcg ctattgaccc cgtcccagtt gatgaagttg aatcgataga
     7201 aaatcaacca atagtagcag tggctttgta taaaaaaatg tttcccaaaa atttaactaa
     7261 attaaaaaat gcttctttcg ctcgaatagc ttttgattcg atgcaagtag caatatgtaa
     7321 tatgtcaaaa aaggctatca aaaaagcaga tctggctatt ttaccagaag tggaggatat
     7381 taatgtcctc gattttgtca aggccaaaaa atacgtagaa ataggttatg aggcaatgaa
     7441 ggcaaaaata gatcaattaa atgaattagt cagttgattt ttgccaagtt tcggatttta
     7501 attctcgaaa agaggtttct ttaattagct gataattttg agatagaagt ttttggcgat
     7561 aatcgaggcc gtgaatttca ctggcgtagg gaataacggt gatttcagac tcggtggaac
     7621 ttatactttt taaatcgtta attttgagat ctggtcgata atatttgatc ggatcagcga
     7681 ctgattcaat aatatagacc ggattaatga gggaggccga aagtgactgc caatcttctc
     7741 gatgaaaact tggattgagt aagtaaataa aagaaaaaaa cagaaaaatt aaagtataaa
     7801 aataattttt gtggagaatt aaacaaagaa aagggattaa atagagaaag cgaaaatatt
     7861 gaaacatggg agtaaaaatg gaaaagataa agccgatgaa aagacttaac caaaaaataa
     7921 aagcgaattt tcgctgatta aaagaacgaa taaaaagggt aagccaaagt gggatggcga
     7981 cgagaagggc aattaaataa taagcccatt taggataaaa agagattctt cctatcgtaa
     8041 atttaaccaa aattaataat aaatttttaa tattagccgg acccaaaacc aaagaccagt
     8101 tttcaactga gaccagtaat gtttttgaga tttgatattg ctgccaaaga aggggtgaaa
     8161 gaattagaaa cgagaaacca ggagctagag aatagataat aaagttttta taattctttt
     8221 tgaaaagcca ataaaaactg atagccgcaa agaagaagac agagccgtaa aaagtggaca
     8281 gagagagaaa gacaagtagg taatagatga gtagtttgtt agttttaaaa gctaaatagg
     8341 cgcaaaaaac taannnnnnn nnnnnnnnnn nnnnaaatag atggtaatta gagaaaaaat
     8401 aactgaagga aaacgaaggg atatttcgct gtatccaaaa atagaagtcc aggcttttag
     8461 ggttaaataa taaaatggcg gatgaaaatc agaaggagaa aatttagtga tgatatcact
     8521 ataggaataa tttttgacga catttgcgga tatcgcctca tcaagccata aagactgatt
     8581 aagagaaagg agacgtaaag ctaaagaaat gattaaaatt aataagaatt ttttagtcaa
     8641 tgaaatatta taaactagaa tataatagga aatatgacca atgaaccact agccgccatt
     8701 ctaagaccaa ggaaattaga cgaatttatt ggacaaaaac atttagtagg cgagggtaaa
     8761 cctttgcgcc tggccattga aaatcatcaa atattctcaa tgattttctg gggcccgcca
     8821 ggagtaggta aaacaacttt agccaaaatt attgccaggg aggcagatgc ggaactttat
     8881 gaaatgtcag ctgtttcggc aagtaaagat gatatcagaa agattgtttc atcaactaat
     8941 cccagattaa taaaaaagat tttattttta gacgaaattc accgttttaa taaggcgcaa
     9001 caggattttt tactccctta tgtggaaaca ggcgccttga ctttaattgg ggcgacaacc
     9061 gaaaacccaa gttttgaagt gattgctccc ctcttgtcca gatgtcgagt ttttgtccta
     9121 aaagaattat ctgaaaaaga aatggaagaa ataatcgaca gatgtctcaa aataataaaa
     9181 ataaaaatga cgaaatcggc caaggagtgg ctgatcaata tggctaatgg ggatgccaga
     9241 caggcaatta ccatgattga taatgcctca actctttatg gtgatttaaa aattgaatcc
     9301 ctaaaaaatg ctctccaaaa ttcctattta cgttttgata aaaaaggcga agaacactac
     9361 aacacaatct cggcattcat taaatcgatg agagcgagta atgttgatgc cgctctttat
     9421 tatctggcgc gaatggtagc ggccggagaa gatccaattt ttattgccag acgaatggta
     9481 gtttttgcct cggaagatat cggactcgcc tcttcgtcgg cactactggt agccaatgcg
     9541 gtttttcgag ccgtggaaac tattggttat ccggaatgcc aggaaaattt ggctcacgga
     9601 gttgcctatt tagctaaatg ccccaaggac aggtcagcct ataatgcctt tatgagtgct
     9661 ctaaaagatg tggaaactta cggtaatctg ccaattcctt taaatcttag aaacgccgag
     9721 acaaaattga tggaagaact tggttatggc aagggttacg acaaatatac taaagaatct
     9781 ttactaccag aaaaattaaa aggtaaaaaa tattttcgag agaagaagaa ataatggata
     9841 aattcgctga aaaatttaaa attaattcgg cacgtttaac aaattgggat tattcaacac
     9901 cgggaaatta ttttttaact gtttgtactt gtaatcacaa taaatttttt ggaaaaattg
     9961 ataataaaca aatggtgtta tcaaaaatgg gaataattgc taatcaattt ttgtctgaaa
    10021 tttctaaaca ttttgccaat gtgtgtttga caacgtcaat tgttatgcca aatcacgtgc
    10081 atcttcttgt ccatgtagag acgccatata tggcgtctca attacaaaaa aatttcaata
    10141 taaacacaaa tcatgaatca tcgattgtaa attttaaaat taataaaaaa ttgcccaatg
    10201 aaaattatag taaagagacg ccatatatgg cgtctctaca agataatcga aaaatcacgt
    10261 tgataaatta cagtcacaaa aaccatccga attattttcc acgattaagc gaaaaatcaa
    10321 aacaattaat tcctaaaatc attcaacaat ataaatctgc cgttaccaga caaataaatc
    10381 caaaaacggt attttttggt tggcaagctg gttatcattg tattattgtt gaaaatgaaa
    10441 ttgaattatt aaaaataaaa aattatatag ttaataattc taaaaattgg caaaaagata
    10501 aattttataa ttgaaatatg ttgacgaaaa ttaataaaat acaggcaaag agtgtactga
    10561 cgaagtcggg actgccagga agtgattatg tggtaaatcc ttataatggt tgtcagtttg
    10621 cctgcatgta ttgttacgcg gcgcagattg cgaggtggaa acaccctggt gaagaatggg
    10681 gaagtttttt agacgtaaaa ttaaacgccc ccgaattatt gaaagcagaa ttagaaaaat
    10741 tagaaaagaa atttaaatcg aaaaattttg gcacaatatt tttttcgtcg gtgaccgacc
    10801 catacacggg aatggaagcc aaatatcaac tgacgagaaa atgtttggaa gtattggccg
    10861 atttcggata tgaaggcgag ataagcgtcc aaacaaaatc gggattaatt actaaagata
    10921 tcaatgtttt taaaagatta aaaaaggttt tggttggttt taccgtcacc accctcgatg
    10981 acaaagtctc aagatttttg gaagtggaag caccaccagt atccgtcaga attgaggcca
    11041 taaaaaaatt aaatgaaaat ggaatttcta cttatgcctt tatcggcccc gtgcttccct
    11101 actttataaa aagtaaaaat aaaattaata aactattaga cgaactgcaa aaggcgggag
    11161 taaaagaagt gtggtttgaa cacattcatc taaactcggc agtaaaggct agattgttta
    11221 actatttaaa aagcgagaat ctaaaaattt tagaagagtt tgagagatca gataatgccg
    11281 agtatcgaga ggatttagaa aaagtaattt acgagtgtct tgaaggacga aaactaaaac
    11341 tcggtcttgg aaaaattctt tatcataaag atttatcaaa aaagaaaata taaattataa
    11401 tcaagcatga aaaatattga taaaaaattt ttaaaaattg ctgttaatca agctaaaaaa
    11461 tcggttaaat tgggtggatt tccagcgggg gcaatagtgg taagagatgg taagatatta
    11521 tctcggggaa taagtattgg gtttgctctt cacgatccta ctagtcatgc cgaaacatca
    11581 tcaataagaa aggcatgtaa aaaaattaaa agtactgatt tgggtcgggc caccttatat
    11641 gcttctcttg aaccatgttt aatgtgtttc caagttgcaa attggtcaga agtgtctcga
    11701 attgtatatg gatgtaaaaa gacagaaacg atggtgaaaa aaaattatta cgaaggaaat
    11761 aataatatta atagaataaa tgtaagaaat aatcgtcaaa ttgaaatgaa atatatccca
    11821 gattttgaaa aagaaatgca agatttaatt aaagaatgga aaaatagata aaatattttt
    11881 aattaacatg tattttgttt atattttaga atgctctgat gggactttgt atacaggaat
    11941 tactagtgat ttggaaagac ggattaaaga acataatagc tctaaactcg gagcaaaata
    12001 tactaagatt aggcgaccag tggaattagt ttattcaaaa aaatatacca acagatcaga
    12061 ggcactaata gaagaagcca gaataaaaaa aataaatcag cgagaaaaaa tagaattaat
    12121 taaaaagtcc ctaaaatctt aggggctttt attaggtttt aaaaaatatt agcgaataac
    12181 gatctgagag tttttctgga gttcgctaaa ccaggtattg acggccgtat tttttatttc
    12241 agtggtaagc tgagttttgg ccagctcctg aagctgctcc ttggtatagg tagttggtaa
    12301 ataggattta tttttggtca gataatcatc aatctgagcc tgggtaattt ccaaatttgg
    12361 attggctaat ttttccgcaa ttttttgaat tttaatttga ttttcgagat cagttctggt
    12421 catcccttcc gcacttaaag ccgcttctag tgtctggcct tgttcaacta ttttagcctc
    12481 aatactggca atttcagtgt cgatcagact tttctcaact tcaactccct gctttttggc
    12541 ttcttgaaaa atcaaggctt cgttggccat ctgttttaaa gtttgttttt gatctgtttt
    12601 aactaaattt tggagatagg taaatcgaga aataggcttg ccgttgacgg tcgctacaac
    12661 tccaaagcga taataaagaa aagctaaaac cgcaagagct aacacaacaa tgaaaggagt
    12721 aaagtttatt ttttttacag gttgagtaat aacgggagct tttttaactc ttttgctggt
    12781 tgtttttttg acagtagtta tttttttcat gtattgagta taacacctgg ggattttgaa
    12841 ataaaagagc ctgatttaag gctcgatgaa ggttaaggcg tagccttttt gattagcccg
    12901 gccagtgcga ccgatacgat gaatataatc atcataggtg gctggctggt cgaaattgat
    12961 gacatgggta atatcatgaa tatcgagacc ccgggcggcc acatcggtag ccactaaaat
    13021 attgagacta tcatttttaa aggatctaat cgtagttatc cgctgacttt gtctcttgtc
    13081 tccatggata gagccaactt taaaaccttt ttgttgtaaa gatagataaa gtttttcagt
    13141 atacatttta gtcgaggcaa aaataagaac ttttttaaat tccggttttt ttaacagatc
    13201 acaaagaact tcttcttttc gataacgatc aactcgtacg acattttgtt caacatggga
    13261 agaggtagtg gttgttttaa cagatacttt tactggattt attaaaaatg tatcaattaa
    13321 tgcttctatt tttttgtcaa cagtggccga aaaaaataag gtttgatgtt tttgagtaat
    13381 ttgggaaaca accgatttaa tatcagcgac aaatcccata tcaagcatgc ggtcaacttc
    13441 gtcaagaacc agagtgtcaa actgagcaaa attgataact cggcgacgac ctaaatccat
    13501 aactctcccg ggagtaccaa taatgacatt gggtccacga cgaatatcca taatttgttc
    13561 gcgcataaag gcaccgccaa tagcgagagc taaatagacc ttaagaccgg gagtaaaaga
    13621 gcgaaaatcg tcttttattt gctgagccaa ttcacgggtt ggcgctaaaa ttaaaattct
    13681 tttgcgagga tttttaatta tttcattaat aagaggtagg gcaaaggccg cggttttgcc
    13741 ggtaccggta ttggcgacgc caagaacatc acgaccagct atgatttcgg aaattaattg
    13801 atcctgaatt ggggttggct ttaggtagga attagccttg atgttggcta aaagctgagg
    13861 attaaaatta aaatcagtaa aggaatgaat tgggttagtt tcctctattt tggtaaattc
    13921 ttctgccttt ttaataaaaa gagccggatt aataatattg ccaaaacgac gacgaggagc
    13981 accacgagat gaaccagaaa aatgattaaa tttagaagaa cctggtcttt ggccaaaatg
    14041 agcaaagtgg cgcacggaac gagagttagt attttgttga tacataataa ttgatgagcc
    14101 ggggcccaca attattcaag cacaacgaaa gaggtgcagg agggcgttcg caaatcagag
    14161 agtagtgtaa cacagttaaa attaaaagca aatttgtagt agaatagaan nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnntcttca agattattcg gaatatcctt ctctggtacg
    14281 aggtggtcac aacacttatc aggttagttt ttctgtagat aaggtatatt ctgtccataa
    14341 agtagttgat gtctttattt ctttggtggc tggacactgg caagagcatg aaaaagagtt
    14401 tagaaaagat actttagttt ttgctgataa tgacggtaaa ataaatattc cttttaaaga
    14461 gatgtgtaat gaactggggg gaaacatcta tgcgaatacc gttagtctcg gacttttggc
    14521 cttcttattg aatttaaata aagaatttgc caaaaagaat attgagcagg ctttcggtaa
    14581 tgattcagct aatttgaagg cctttgaaaa tggatataac tgggcggaaa aaaactgtca
    14641 aagattgccg gtagacagag gtaaggattt tgagaaaaaa attattgtcg atggtaatga
    14701 agccactgga tggggacttt ataaagccgg ccttcagatg tttgtggctt accccatgac
    14761 tccagcttca ggtttacttc attttttagc ccaaaatatc gatgaattta aaattaaggt
    14821 aattcatccc gaagacgaaa ttgccgttgc ctcaattgcc tctggggcct caatggcggg
    14881 agctagaagc gcggtaggca cttcaggtgg cggttttgcg ctcatgacag aaacaatcag
    14941 ttttaacgct caggcgggtc taggagtggt ttatttcctc tctcaacgac ccggtccggc
    15001 aacgggaatg ccaacttgga cccaacannc tggccgcagg tgatcacgaa gaagcctttg
    15061 aactgggaaa attatcttta aatttagcca ataaatttga tattcccgtg attctacttt
    15121 cagataaaaa tttatcagaa tccagcgcta atactaaaga ttttaataaa gaaaaagaag
    15181 aaaaaatcga aagtcaaaaa gtaattcctg gaaagggaca aaaaataaaa ctatataaca
    15241 gttatgaaca tgacgatgag ggattttcgg tcgaaggggc cgaggaaagt aaaaaagcca
    15301 tggatttgag actgagtaag gtagaaaaaa tattagaggc tataccgaaa gtaaatttat
    15361 acgggagcga tagagctaaa aagttaattg tgtcttgggg ttcgactaag ggggcaattt
    15421 tggaagcact caaagaaatg gaaaataagg aagattacgc ctttttgcag attaaaggga
    15481 tgtggccact tgatccaaag ataagtcaaa ttataaatgc ttttaaggaa aaaattttgg
    15541 tggaaaataa tgcaaccgcc caactggaaa cactgttaaa atctcaaatg acaataggat
    15601 ttactaaaaa aatattaaaa tatgacggaa gaccattctt tccggaagaa ataaaggagg
    15661 ctctaaatga ctaattggcg atcggggatt gcccctactt ggtgtccagg ctgtcatgat
    15721 tacgttattt ttgccggact acaaaatact ttaacaaaat taaatctgga tcaggacagg
    15781 acagtgattg tctatgacat tggctgtatc ggcaatatgg ctgattttgt taaagcttgg
    15841 ggagttcatt cccttcacgg tcgatgcatc ccgacggctg tcggaataaa actcgccaac
    15901 cccgagttaa atgtcattgc tattggcggt gatgggggaa tttatgggga aggacttagt
    15961 catttaattg cggcagccag actaaatatt gatatcacag tgattgtggc taataaccat
    16021 ctttattccc taactaccgg tcaaagtagt ccgacaacac cgaagggaac aaaaactaaa
    16081 tcaaccccca agggagtacc cagcattgct ttaatgccag tggagctgat taaaacagta
    16141 aatacagaag caaatgccga acatattgac tgtaaagagc cattaaaagt taacgaggca
    16201 atggagaaag cgattaccca taaaggattt aatctgctcg atctagaaca gctctgtgtt
    16261 agttttggga aacagttaaa gtaaaattaa aaaaattcta aattctaaag tctaaaaata
    16321 tgaaaaagaa aaatcaagaa atgctcgttg tatcaaaatt ttttgaagaa gcaaacgatt
    16381 ttgaaaaacg ctataagtct ttaaaaaaaa caataaagag tatcaaaaaa tttaaagaag
    16441 tgcaatttaa aggaaaaatt gccgaggctt aaagaaaaat aaatagaaca agagccaaaa
    16501 ctaaagtgta gattccaaaa taccaaaact tattaccggt gataattttt tccaacagtt
    16561 ttaaggcaaa gagtccaata aaaaaggtaa aaacaaaagc aattaaatta ttagaggaaa
    16621 gattattgga ggtaaagata ttttttgttt ctaaaagaat tgccccgacg gtagcaggaa
    16681 taaacagact aaatgaaaaa ttaaaagcat tttgggatga aagacctagg agtaaggcag
    16741 caaaaatagt tcctccactt cgagaaatgc caggtaagat agctatcgcc tgaaatatac
    16801 caatgataaa ggccgataaa taggttaatt ttttatcctg ttttttaata aacttggttg
    16861 ccaaagtgta aatagaagta aagagaaaga ataatggcaa taattttgga aaagcggcga
    16921 gtgaggaaat ttgatcttta agaaaaaaac cgatgataat caccgggata gagccaacaa
    16981 taatatattt taaattagat aaaaaataag ataatttttt tctaaagaaa aatagaactg
    17041 aaataaaagt ggctagatgg agaaaaatat caagagttaa tgatggcgta aaaccaaaaa
    17101 ggtgctgaag taaatttaaa tggccatcag aagaaatggg taaaaattca gtaacccctt
    17161 gaacaaaggc aagaattaaa gattgaatta aagtcattaa ctattttaac gcaaaaactc
    17221 tgtggatgga tttggtatat aatggcagta taagttaaaa attactttac atatgcctga
    17281 taacagatct gaatttaccc caacgatgtc ttcttttggg ggctatgttg ctgatttact
    17341 ggggacatta agacaagttg aaactggtgt accttacgag gattcactta atccggatgg
    17401 aaatccattt cggggaatag attccccagc agaaatgata aacaagttcg cggaggtaca
    17461 taacggaatt aataacttgg aaattttagg aagcagtctt gacagttttt atggagttaa
    17521 agccggggag aaagtaccag aaaatttgcc agaaatttat accgaagcca ggagaagtat
    17581 tgacgagagg ataatagcag agagaacagg acaacaaaaa taatttaaca taaggatatt
    17641 aaaaaactcg cctggtgacg ggtttgagtt ttgaatgatt gcttcgtcga agtacctcct
    17701 cgcaatgacg agatgacaca atgacgagat tttaacgttt tggtgattgc ttttgtcgtc
    17761 tcgctttacc accggtaccg accatgcgac gttcacgaac acgagcgtca acagtgagca
    17821 aattggcctg agctaagaca ggagtcatgt caggattagt tttctttaga gcacgagaaa
    17881 tggctaaaat taaggcagcg agttgaccgg ttttaccgcc accaacaatt ttagcgttaa
    17941 agtagtattt attttgagtt tcagtaacaa caaagggtaa atctaaacga gaaaaagtta
    18001 aagggaaata tttgtttaga ggacgagtat taatagttga gtcccctttg cctttaaaca
    18061 atttaacagt agctacagcc gctttacgtc t
//
LOCUS       DHGB01000044            5526 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_43914, whole genome shotgun sequence.
ACCESSION   DHGB01000044 DHGB01000000
VERSION     DHGB01000044.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_43914"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    2239..2296
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1703 a         1094 c          879 g         1792 t
ORIGIN      
        1 aaattttatc caactctttc cgacttaccc gagattcctg acttagtcgt tattgccatc
       61 cccgccaaat tagttactaa tgttcttgaa gaatgcggtt ttttgggtat taaaaatgtt
      121 ttaatttttt cagccggttt taaagaagca ggtgaagaag gcgccgatct cgaaaaaaaa
      181 ttaattgaag tcgctcatca ttttcactta aatattctgg gtcctaattg tcttggtttt
      241 gcctctactg atccttctct aaacgtcact tttggccagg tagtgaccaa tcctggcaat
      301 attagattta tatctcaatc tggcgctcta gccgccagtc tttttgattg gtgtcaaagt
      361 accggcctag gttttaacaa tttaacgact atcggcaaca agtcaattgt caatgaaaat
      421 gatattttaa aatattggct cccagcactc acgctcccca atgatacatt aaagccagtt
      481 tctttatatc tcgagtccat tgctgatgga caggaattta ttaagataat taagcaaatt
      541 actcccttca atccagtctt tatgttaaaa cccggtaaat ctccgtcctc agtagccgcg
      601 atgcgttctc acaccggttc aattgcgggt gaggataaag ttttagcggc ggcactttca
      661 gaagcgggaa taattcgctg tcaggagctt ggggattttt ttgatttagc gcaggctttt
      721 tcctggacga gtgttcccct tggtccggga gttgccgtta tttccaacgc gggtggtcca
      781 gctgttttaa cagctgacac catttctagc ttaggtctgg aaatggccgt ccttagttct
      841 caaactcgag aaaaattaca agaatctctt ccccgaatgg cctcaattat caatcctgtc
      901 gatgtcctcg gtgatgctct ggcccagcgt tttggtgaag ccctcgaaat agtattacag
      961 gaaaaatcgg tagattccgc gttaattctg cttactcccc agttgatgac tcaggtcgaa
     1021 aaaacggctg agattattgg tagcttgtct aaaaaatatc ccaaaccggt tatttgttct
     1081 tttatcggtg gcagtagtac tgccactgga caaaaaattc tgtatcacaa ccagattccc
     1141 aactatcctt atcctgaaag agctgttaaa gttattgctt cgatgtggca gtggcaaaaa
     1201 tggcgacttt ccccgctttc aatttctatc cctaaatcaa atttggccca taaattaaaa
     1261 attaaagaaa taattaattc tgccctgcaa ataaaattac ctactcttga caatcaagcg
     1321 gcttcaatgc tgatggaggc gataaacatt cctgtgcccc caagcaaatt aatacaatct
     1381 tatgatgaag ctcttctttt ttcccgggag aattcttttc ctctggttct caaaatgtcc
     1441 gccccaggac tattacataa ggctgatatt ggtggggtaa ttacaggtct taattcggaa
     1501 actgagctaa aagccgcttt tgataaaatg acgagcagtg ttaccactct gtcaaaagat
     1561 ttttctcaaa aaattgaaat ccaaattcaa aaacaaatca gtggaggaat tgaagtaatt
     1621 ttagggataa aacgtgatcc aagttttggc ccagtttttc tttttggggc tggaggtaaa
     1681 tttgctgaaa tattcgccga ttctaatttg actcttttgc ctcttaattt agaaaaagca
     1741 aaaaaaatag tccagtcaag taaggtttac aaactcctct caggctttcg cgatgatcct
     1801 ccttacaagc tcgacaaact ttatcaaata atgctgactc tcgggcactt aatcactgat
     1861 tatccggaga ttcgtgaaat tgaaattaat ccggtcataa ttactcacga agacacctgg
     1921 gctcttgacc caaaagtact gcttaaatag cttttaacaa gctactaata gtttatttac
     1981 atgtccagcc gccatctact ggtagaacta ttccagtaac ataagcagaa tcatcggatc
     2041 ctaaatataa ggcagcctgt gcaacatctt caggttttcc cacgtatccc aggggtgtac
     2101 tgctaataat aaatttagaa aaattttcat cttccaaaaa agtcttagtc attgctgtag
     2161 ttataaaacc tggagcgata gcattcaccc taatacctag agccgctaaa tcaagtgccg
     2221 ctgatctagt caaattagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnntaac tccataaaaa actccggaca agttaactcc gatcactttt
     2341 gcccaatctt catcagtgca ccccatggct ccaccaggag cactgatacc ggcattattt
     2401 accataatat caagtcggcc atgtttttct acagtactat tcactaaact atttacttcc
     2461 atagcattcg caacatcaca tttcacaaaa cttgcttttt ctccataatc agcaattcct
     2521 tcaacctcat tgacatcaga aaaaactaca gttgcccctt cttgtaaaaa tattttggca
     2581 attgccaatc caattccact tgaagctccc gtaacaatag ctactttatt ttctagtttc
     2641 ataaaaattt aaacaattaa taaaaaaaat aatactttat tttttaggct tattaaattt
     2701 ttcatttaat aattttaatg tttttccttt atctatatcc catttgttta aataaaaaat
     2761 atagacaatt tccaaaattc ccatagtgtt tatcgtcaag agaattaaaa accataacaa
     2821 ttgcttctta ctagctgatt tccatagcgc cagccctttc cagactgtaa cccaaattaa
     2881 ataggggata ataaaatacg gcttgtttaa aagtgtttct attgaattca taatttatta
     2941 tactgcaaat agacaaatgt tactctataa ataatgctct attttctttt ttttatttta
     3001 tttttaattc tcagcctcct ctttcttttt ttcttttacc aaatatatac tctttttctt
     3061 cagcctgggg cagtttacta tcctacaact gacagtagtg tcaaggaaat gttgaaaata
     3121 tcccagctta cttcaaaaga tattctaatc gatcttggtt cgggggatgg tcgaattttg
     3181 attgcggccg cccgtcttgg agctcgagcc ataggctatg aaataaatcc ctttttggtt
     3241 tatagatccc ggcgattaat aaaacaatta aagttagaaa agcttgtttc agttaaatgg
     3301 aaaagttttt ggaaggcaga ttttaaccag gcgtcaatta ttaccgttta tctctttccc
     3361 catttaatga aacgactcca aaaaacatta gaaaaaaaac tcaagcatcc tgtgcgggtg
     3421 gtctgcaacg attatccctt tcccggtctc actcctgtta aaaaatttaa aaatatatat
     3481 ttatataatt tcaacagctc tattgcacta agaaaaaaat aaattgcggt taattcactg
     3541 cctgcccgtt tacccgcctt tggagggcct ctggagggga acgattttcg aactttttta
     3601 gttgtttgtt tttctataaa tttacaataa tattatattg ttaattgaca aaaaataaca
     3661 tcattgttta aatatctaaa tggaaacaaa caattcagaa gtgtcaagag aattaaggtt
     3721 ggaacatctt cgctctcttg aaatcgagcg attaagagcc gcccatccta aagattggcc
     3781 tgaaagcaag agacaggagc ttttaaattt acaaaaatct ctttttggtg aacccttcaa
     3841 tataactcaa gaacacaagg atgctcttag gcgtcagtat atcgctgaaa ctggagacaa
     3901 ccctcctctg acagataagg aacgagaatt aattcgagaa gctaattctg caaagtaaag
     3961 ttaactcact ggaacgattt tagaactaat tcactagatt ttgcggtttt cccttaatca
     4021 caaattcaat attttctacc caaattctca tatcttcaag aaatgcttct tttgtaaacc
     4081 aggctattgg tggtgttact aaaatattac ctccgaaatt atttaacttt aaattattga
     4141 tggtgtattt gtctgattca aaagccagtc ccgataattt tccacttttc accaattgtg
     4201 cagcataatc taaatcaaat aaatcatcac ctgtaatact gactataaaa gctccttgtt
     4261 ttagtaattc caatttatct ttattaataa tatttttagt ttcttcattc cttgccaaag
     4321 ctggaaaaat aaaatcagcc gtttttagaa cctcatcaat ttttttatat tgaaatcgat
     4381 catcacgaga attttttgac cagtaaacaa cattcattcc gagtgatttg gtcaattctg
     4441 cgattcttgt tccgattgaa cccaatccga ttattccggc agtttttcct tttatttcaa
     4501 aaccttgatg tttatcataa tctaatttcc aattattcat aataattaat ggaatttttt
     4561 taaccaaatt caacatcaaa gaaatagcat actcagccac agattcagtt gaatatttag
     4621 gaacattaat taaatctatt cccttttttc tcaagtattt cccatctatc caggaaaaac
     4681 tagttgtcgg gacacataca gctcttaaat ggggtattct attaattaga tcgtttggaa
     4741 aagaccattc ggtggtatca gggccaattg ccaaaatctt ttccgtttga tcaaagagtt
     4801 cggggttttg taggccttct tttgtttcaa gaaaaacaac atctcctaaa ctctctaata
     4861 aagaaatttg agacaaagaa aaatcttttt tagggttaag aaaaataatt ttcatatctg
     4921 gtttgatgat accttcaatt tgaaaaaagt tcaaatccat ttaagagtaa caagttaaat
     4981 agataaaatc tgaacctaat tttcagtgtt accccacatg gattcgaacc acaattccta
     5041 cgttcagagc gtagtgtcct accattagac gatggggtat agatagagca ttattaccgc
     5101 caatattata caatatagct atgaaaacta ttgaagaatt aatcaaagaa ttaaaattga
     5161 atcccgagca gtccgaggtc gtcaaaaact attttgagga tttggttgtt gagctacttg
     5221 aatctttaaa acaggacaat cttcaaaact ttgaggaaac catttcttct attcgcaaaa
     5281 gttaattttt cccaaaaaaa tatctggcct ctgccacgct tgtcgcttcg ccatgtcctg
     5341 ataaaataat cgtatctttg ggtaaattaa gtatttcatc tatgctttta tatatatctt
     5401 tggtcgaaga ataatggtga tttgtttgtc ctctcattcc ttgctcaaaa atagtatcgc
     5461 cagaaaacag taaattatct gccaggaaag ccaagccccc tggagtatgc cctggagatt
     5521 ttaaaa
//
LOCUS       DHGB01000045            5357 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_82124, whole genome shotgun sequence.
ACCESSION   DHGB01000045 DHGB01000000
VERSION     DHGB01000045.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_82124"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    3383..3526
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1782 a          778 c         1153 g         1494 t
ORIGIN      
        1 ttattggaat ggtaatagtg gccaggagag aaagattaat agatgagtag tttagtagaa
       61 aagaagtaaa agtaagaaat aataaaaata gataaagaaa taaggggagg agataataaa
      121 aggggtgaaa aaaaattaat gagagtagat atatagaaaa aaatgacggc agaagataac
      181 cgagacgaaa gctagttttg gggtaaatac gggcgaaatt accccgatga agtgcgtagc
      241 gggtaatttg ctgaaggtga ggaataatga cattacgacg atggtggtaa acaataattt
      301 tggggtaata aagaattttt ttattaagtt tatagactaa atttgaacat aaaatagtat
      361 cttcaccggg ccagtggtga gtgtcgaagc cttttacttt atcaaaatct gtttttttga
      421 caattaaatt gaccgaaggg aaatcatcaa caaacctttt tgatttaaca gagttacgat
      481 aggtaccggc tcccccactt cccaaataag atgaccagac gagacctgag gctttttgaa
      541 aaatattatc ggagggcgga gtcagacatg ggccacaaag agcggcgata tctttggtaa
      601 ataatttact ggcatgtttt aaccaatttt tactgggata tgaatcatca tcgagaaagg
      661 ccagatactg gcctttggct tttttggcac ctaaatttct tttaatagcc ggggagaccg
      721 gaccggatat tttatcggta atgacgagaa cttcgaatga tttaaatgat tgttttttta
      781 accgccggag agtttccttt aagtattcgg tttcacttct aacagggata ataaccgaaa
      841 ataagggggt gttcattgac cggtattaat tttcatttct aaatcggcgt cataaatcca
      901 ctttcttttg tttttatcat catagtaatg gagaattttt agtcgataaa aaacnntcaa
      961 aaacataatt taatttaatg ggcgcttcat aaattttatg aaaaccaaga tggttggcta
     1021 cggataaaat ttccagatca atggcataac ttttgataag aagacggggg agaacttttt
     1081 ccagtacttg ccgacgaaat atttttaatc cggcctgagt gtcgcggaca tcgataccga
     1141 ggagaaattt agaaattaaa tgggcgccaa aactaataat ttttctgtcc agaggataat
     1201 taaccttcga ggccggatgg cgttttgaac cgacaataat atcggcttca taccattcca
     1261 tgtgctctaa aaccatcgat aaaccgttgg ggtcaatttc catccccgaa tctataaaag
     1321 ccacgtaatc cccctttgac cgggccatgc catagcgaag agcgtagcct ttgccatggt
     1381 tttctttata gccgattacc tggagatttt ttaaatgaca ttttttagct tcagtataag
     1441 tattatccac atttattcca tcaacaacga gaattatttc ataagggtac ctaatttgtt
     1501 caagagtatc gttaatagaa tacacatctt ggcgaatggt cgaggcctgt ttataggcag
     1561 gaataataac tgagagaaaa tgatttttat ttttgagcat cgatttcgac taatttatag
     1621 acagccaaag tctgtatggc agtttttttc caggaaaaaa attgagctct aatttggccg
     1681 agttttgaat atttttgacg aagtttttta ctggtccaca tttttttaag tgagtcttca
     1741 attgatgatt gagaataagg atcaaaataa agaccattaa aatccattat ttccggtaaa
     1801 catgactcct gcgaatagca aaccggagtg ccacactgca tagcttcaat taaaggtagg
     1861 ccaaaacctt ccgagaatga aggctggcaa tatatggtag ctaaattata tagagccggt
     1921 aattcttcat cagggacaaa gccaagaaga tataatagat tgattcgctt cgctcgcaat
     1981 gacgcatcaa ttgactggag atggagaatg tcctgagtcc aggggtgagg ggggacctgt
     2041 ttgaccgcgg ctgaaccggc aatgaccaag ggatatttta atttaaggca agctttgact
     2101 aaagaaggaa tatttttgtt ccagttgata tcgccgacat ataggacgaa tttattgggc
     2161 agcttatatt tatttttgat tgtagataaa aaaattttat tattaatttt tttaaaattt
     2221 gatgaagcgg cttcataagt ggtgtaaatt ttatcggcgg ggtatttaag gtgatcgagg
     2281 atttgatatt tggcgctgtg tgaggggcaa atgatgtaag cagctttttg taagcggtgt
     2341 ttttgaatta accattttat ttcaccctta acgccaacgg gaaaatgttt tttaaagact
     2401 ctggggatta aatcgtgaca ggtaacaata tagggtttat tttgggaagg cagggttaat
     2461 ttaaagggat caaagtaggg atagtgaatt aaatcataat cttcaatctt caatttacaa
     2521 tcttcaatta aggtgatttg ataatgttta aagtcgggat ttatcttaat ttctgattgc
     2581 agagctttga ctaaattacg ggtgtagtag ccaacacctc gtttggaatt tccgtcattt
     2641 agtggcgaaa catcaatagc gattttaaat tgtttcatag taaagctcgt gaagttttat
     2701 ataggcaatg ggaaagcgaa gagaagaata ccaggcaaaa acaaaaccgg gaaagccgtc
     2761 aacataagcc cgatgacgaa aataggctag aaaaaaccaa taaattggtt taataataaa
     2821 ataattgaaa aaatttaagc aatttaattt aacccctgat ttttttaaat caccggcaat
     2881 taaggaagtg taacgatcat ttcggaggag atatcggcta aaggttgggt cagcatagtg
     2941 gagtaggtct gcatttagag ttgacacagg acccttaatt tcggcttgtt cgtggacgtc
     3001 ctggcagggt aagtgtccaa agctacggcg ataaagtcta atggtgggat cgggataaat
     3061 tccaccttta gtcaaaaatt taccgagaaa aaagtttttt cgcttaatcc agaagccatt
     3121 aaattttgta tctttaatcg tatttaaaat ttcgtctcgg agctctgggg tgactacctc
     3181 atcggcatca agctgaagaa tccagtcgct ggtacaggcg tcgatcgcca tttgtttgtt
     3241 aaggtgaaac atcggcttat tgtcggtaga gatgacgctg gcgtttttat attttttaat
     3301 aattttgacg gtatcatcgg tagatttacc atcaacaaca attatttcat ctacccaatc
     3361 tgtaacggcg tcaagacagc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgaa ttttgacggg
     3541 atcgtatttt gaataaaaaa gatagagaat ataaggctga tcgtatttat cagtcagata
     3601 gatattttga taattatttt tttgtgattc gataaagggt ataatttctg aaaatcccct
     3661 attccaggca aaagaataac gccggggagc atggatgtaa taggaatcca gataatagan
     3721 nagggcaaca agaatcgata aaggaatgac catagggaga gcccgaaggg cggagggggc
     3781 ctgaaaagta agtgaagagg cgaggggaga tattagaaga aaaataataa ctaagttctt
     3841 tagagctttg gagctttggg actttagaag agagtaaaga ccccaaaaaa gaaagggcaa
     3901 ttcgattaaa tacatttggc ccatatcggg attttttgac cgagggacct cgtctccatt
     3961 tataaacaga aaatttaaat taaagtgaga ggtatatttg tccatccatg ataagacata
     4021 aagaacgcgg cgattgtgca tcgcccggtt gattaacttg acattaccat gatggtttag
     4081 aagctcttca gctcgagaaa gtggaccggt atcggccatt agaccgactc cgcccaggcg
     4141 agttgtacca ccgctattca gaaatgacat aaatacggga atagtaaata ttagagccaa
     4201 aaaacaggga ataattagtt ttttagatga gtagattaat agtttgaagt tgaggagaaa
     4261 taaaactaaa acgattaagg gggaaataag ccgggcagaa tgatatatat agagcgaggc
     4321 aatgaaaggt aaaagaaata gatagcgata aatggatttt ggggaggcaa taaatttata
     4381 gaagaaataa attcccagaa gaataaaaaa tagggcggtg ctgctttccc aggcgccgcg
     4441 ggaaaattga agatgccagg gagagatgga taaaacaaaa gatgaaaata aagaaagtct
     4501 aaagtctaaa gtcaaaagta aaactaattt atataaaaca taaatggtta aaagggagaa
     4561 aattagattg gggaggcgga cagccagagg ggttaaacca agaactttga taaaagggat
     4621 ggccaaatag acgtaacctc cgggtttaaa gtcgccaaag gatttaaagt gaaggggcca
     4681 ggagagacca tgttcatctt ttcccgtttc tattagggaa taggcgttgt agccaagggc
     4741 ggcttcatcg ggatttaagg aggggagtct gtcaatttgc caaaaacgga gaattagggc
     4801 aaaaagtaat ataaatagta ttttagggat ttttgtaacc ataaaaggta gaaatattat
     4861 taattggtga cagtggagtt aataaattta gatcaatccc ctgccgaatc gcatcgtcgt
     4921 aggaaaagat agctagaatt ggtttacttt gaagctgtaa attggaaaaa ttgggggttt
     4981 caaaatttat attatcaatt ttggaaacaa ctcccgtatc aacgccactt aaagttaaat
     5041 tcgcctgaga ttgataattt cggggtggat actgggaata aaaaaggtaa taaatgtagg
     5101 cttgaccata aaaattagta aaataaatac ttctattttt gccattttta attacataat
     5161 ttaccgcttc cctatannag gcgatttttt gaccatatga tttagataaa gatcggtgta
     5221 ataaacaaat gaggctaaat atccaagaaa caataaccaa gaaacaaaca atctaccatg
     5281 tttaaatttt ctaaaccaat ttataaaaac acttaggcca agggcggaaa agtaacatag
     5341 gggaatggaa aatgaca
//
LOCUS       DHGB01000046            4055 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_70582, whole genome shotgun sequence.
ACCESSION   DHGB01000046 DHGB01000000
VERSION     DHGB01000046.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_70582"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1422 a          641 c          895 g         1097 t
ORIGIN      
        1 gaaaaggtaa gaccagaaat gagccaaaag gagtttgggg agatgctgcg ttttaatgag
       61 gagctgagag taaaaatagc taggctagcc tatttaccgg aactgatgga ggcggtcaat
      121 caaaaaaacc aagaagcaaa gctgatgaaa agccgagtag acaaccttgg cttaaaaatc
      181 agtgatatag cgacaaaact gggtttgtgg attcagggaa aaagagagcc gagactagat
      241 gataaaaatg ccaaaagact tttttcggtt atccccgatc tcgaatatgg tctaaaaaga
      301 agtcgggaag gggctaaatt tagcctgagt gaaagggaag aacaaataat agatcataag
      361 gacgtcaatg gagtgggagt gctgggagag ctgagggaaa tgatcgaaac tgaatttgaa
      421 tatagactag taactagtaa attagataaa aagattagta gtcaagcaga attgttgtct
      481 tatgtttata gtcccaaggc agaaattaga aaagcggcat atacggccct tcttgaaaaa
      541 caaaaaaaga atttagataa attttttgcg atttatcagg gaattgtcaa agactggggt
      601 tacgaagcta gactgcgagg gcacatttcc cctatttctg ttagaaattg ggctaatcac
      661 gttcccgaca aggcggttca aagtctgcta aaggtctgta gtaaaaacat cggtattttt
      721 caaagatatt ttaggtggaa agcaaagaag ctgggactta aaaaactgca gcgctttgat
      781 atttatgcgc cactgcaaag taaagataaa aaaatggatt ttgaagaagc aaaagaaacg
      841 gtactcaaag ccatgaggga gttttcacca ggatttgaaa gcaaggccaa aaaaattatt
      901 gaggaaaaac atattgatgt catgccgggg aaaaataaaa gaggcggagc tttttgcgcg
      961 accgtagccc cagagattac gccctatgtg cttcttaatt ttactggtaa atcccgtgat
     1021 gttagtaccc tagcccacga actgggacat ggaattcatt cactttatgc caggaatcac
     1081 tttccctcaa gccaacaagc aaatttaccc ctagcggaaa cagcctcaac cctctgtgaa
     1141 ctaattttat ttgaaaaaat gatggattca gaacaagaca aagagatcaa aaaagcttgg
     1201 ctctcagata aaatggctga tgcttatgcc accatttgcc gacaaaatta ttttattaag
     1261 tttgaaatta aagcccacga agaaatagaa aaaggggtta gggcggaaga tttatcaaaa
     1321 atatatctgg agactttaaa agaacagttt ggcagcagtg tccagattga tcccctgttt
     1381 aaatatgaat ggagctatat ttcccatatt tttgaaagcc ctttttactg ctatgcctat
     1441 aattttggcg aactggtatc ctacgccctc ttcagcaact ataaaaaaga accgaaaaaa
     1501 tggctaaaaa ttattgaaga gattttggaa agtggaggga gcgaggatcc acaaaaagtt
     1561 ttgaaaagag ccggggtgga gataaatgat gaaaaatttt ggcaaaaaag ttttgagacc
     1621 atcgaaaagt ggcaaaacga actggaaaaa ttgtgataga attgttacag acgtagtact
     1681 agttaagtag tactacctat aaaaatatga aagaagaaac aaagaaaaaa ctaatggaat
     1741 ttttttctaa atatccccta aaaaaataca aaaagggaca gatagtaaca gagagcaatg
     1801 aaagttttaa aggcgtttat tttgttaaaa acgggtatct gcgggtttat gacgtcagag
     1861 ccggagaaaa agaaagtgga atccaaattt ttaatccatg gtttttttta tcactaatta
     1921 gcgctaaaac agggcttaaa agccgtcatt ttatcgaaac aatgagcccg gttgaggcct
     1981 ggatggcacc agccgaagag tttgaaaaat ttctgagaga aaatccgaaa ctggaagacg
     2041 aattaaacaa gctgattatg gaaaaatttt tggatttgac tacttttgtg tcgcagctta
     2101 tttccggaaa tgcttattta aaagtagccg gattaattta ttcgatggcc agagaatatg
     2161 gcgtagctaa gggtaaacag gtgagcataa aaattaaaat aactcacaaa caaattgcga
     2221 ccttaaccgg actgaccaga gaaacggtaa ctctacaaat gctaaaattg gaaaagaatg
     2281 gcgtgattga taatgacaga agacaaattg tggtaatgga tataaaagag ttgaagaagc
     2341 tactgggaga cgagtaagtt attcacggca acagtcaagg attttgccga tgataccact
     2401 gaccttacca agaaaatctg atttttggta agagagagga gaatctttgc tttttttggg
     2461 ctcaatgaat tgagccccta cattttttac tagacctaaa tttatacccc agaataagtt
     2521 gtaaagatga taggattttt caaagaagct aagagcgctt tttttgtcac ccttggtctg
     2581 agccttggta ccgacttcaa aaagacgcat ggaggcggct agaaggtgtt tgctggtaca
     2641 ccagatctcc ccttccggat ttttgacgat aagttttaat aattctttgc gcatttcacg
     2701 ggtttcgttt aaaagatcaa aatatttggt tttgccagtt ttactggctg taaaaaaaag
     2761 atgctcttcg atactgatta aattcataat agcgatagat aagtcttcat cggaagaaag
     2821 atcaagtttg tttccggctt cggctttttt gattttgtcc atgaagtttt ggagatcaag
     2881 attgtttgtc atgggtgtat tttaacaaaa gcttataatt taaaaattag tttgaagatt
     2941 agggtagata aaagaaccaa agaaacagga aaaacaaccc tagcataggg aaatatcgtt
     3001 ttgccaccat tgcgtttttt taaaaataag tataagtggc taccaagagt catggcaatt
     3061 aaaccaataa tctggccaaa aacaatttta tcgagaccca aaagacgatt ggtggcggcg
     3121 ccgatttggt cggttaattg aaaatagaga agagtgagag caagcaatag aagagcaaaa
     3181 acagattcgt tttttaaaag ctttattttc atttttggag ccagccaaaa agcaatggct
     3241 gtatttaagg cggaaatcca aattgaaatt agaaaatcat caatgccaag ctttttggca
     3301 atgagcatac caccgccaac ggtgacaata cagacaggac actgagcata agctggaaaa
     3361 actagaaacg agaaactaga aatgagaaag agaaaaaaga aaagaaaata aagcataggg
     3421 ttattttaca agaaaagccc ccgagaaaca tcgggggcat tttttaagca caagtacagc
     3481 cggaaccgtc ttgacaacca cagttacaat cgggggtgca gtgacatttt ttgtctttat
     3541 ccattctaag attaataatt aattactaca agtgtagtag tatataggga ttgtgtcaag
     3601 aagttattta agctggtgtt ttttggcgaa ttttttgaag gcgaggagaa gcgagtcacc
     3661 gtaagattcg aagagccgag caaaaagatc ctctagggcc tcggcagcaa aggtggattt
     3721 gtcttgaaga gcttcatatt gataagtatt gcctattaat tcccttttga taagtttttt
     3781 gtcagccatt ctttttaaaa cagtcatgat ggtggtgtag gcgtgaccgt tgttgatctt
     3841 tgctaaaact tcggatggct ttaggggagt agagctcgac caaagtactt ccataatttt
     3901 ttgttcaagg ccaccaagca ttttacattt agcggtactg tgacagttgc aattcattag
     3961 gtttaaattg taacacttat ataattttaa gtgctacgac ttctttttgt cgaggcggtc
     4021 aagctttaga gccttgactc caaaatagac cacga
//
LOCUS       DHGB01000047           14660 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_31420, whole genome shotgun sequence.
ACCESSION   DHGB01000047 DHGB01000000
VERSION     DHGB01000047.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 14660)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14660)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14660
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_31420"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    5506..5535
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4769 a         2273 c         3756 g         3826 t
ORIGIN      
        1 tagttcgagt ctatcattgc ccacagtgaa atcgtgtata tatattttga agtcacaaaa
       61 gaatggtcgt ttttatgttg gcagtacaag taatttgaat agaagactag aagaacataa
      121 ttctggaaaa agtagatata catcagaaac taagccatgg aaagtggttt ttagtcaaga
      181 atttgaaaca ttatctaagg caaaaaaggt tgaatattgg ctaaaaagac aaaaagatgc
      241 agattttatt gaaaaagtaa tttttgaagg aaaattgaca aaaaagttta actagagcac
      301 tccggtgtcc accggagggg tcgatagttc gagtctatca ttgcccacaa aacaaccttt
      361 tttgtagtgt taaaattaaa tatggaagag aaaaaattta atccaattaa gtttttagga
      421 atgatggcgg ctttttttct gataattttt ggaagtttta ataatgctcg gagaacggtg
      481 aaaatatttg tggataaaat taaaaagaaa taaagtaaga ataagctgac tattttatta
      541 gactcttaat aaagttccct ctccagatag aaaaatcgtt aagtgtaact ttaccgttac
      601 aatcaaaatc agcaacccaa ttatttttgg ttattttccc cagacctcca ctgataaatt
      661 cacttctcca aatagaggaa tcattgagac tagtgtttcc tgaacaatca gcatcgcctt
      721 ttaatttagc ctcaggtttg ccagaacatt gaggacaaag agagagggga agagtgggag
      781 ataggatttt agtaagggtg ggggtggggg tagaagtgat aagtgtgttt tgataaagtg
      841 ttttgacttc agaggcagaa agagcttttt tagaaaaata aagtttgccg attgatccgg
      901 taaaatcctt tccaatgagg atgtccgaat aaggatcgat agtgaatcca ctgacaggag
      961 cagtagaggt ggcaacttgg ttgccgttta gataggaagt taacttgcta ccgtcgtaaa
     1021 cacacaagag atgattccat tgaccaaaag taaaatgaag agaggcggcg gtattgggag
     1081 agtaccaggg agttttgtcg gtagcaagga tacaatggcc ggtgccattt tgttttaaaa
     1141 caaactgaaa agaagtaccc ttttttaaag ggaagagatc tttttgagat acagaggggt
     1201 taaaccaacc tccaaaactg aattgctgac tgtgactggt gggagtatcg ccggcaataa
     1261 gataattttg gctgtgaggg aaagtataaa tttcagcttg atattgagaa ggagctttga
     1321 ctttttttag aaagtattga tattggctgt cataaggacc attattgctt tcggcgggaa
     1381 ggcgatagtc tggaacgtta ttttggtgac tgagtatttt gcggattgac ttaaggtaat
     1441 cgaaattgta tttgtgagtg ggagcaatcc aatgaccttc accgtattca ttccaggcat
     1501 cagcaatgat tactttttta ccaagatctg agttaatatt gggaataata ttcgatttaa
     1561 gataactgat taaattttca tattttggag gagaaatagt ccagagaggt tgattgacac
     1621 cattggccat gggattagcg ttggcaatga agggaaggac ctgattattt ttaagccaat
     1681 cgtaggattc tttcataccg gtaagagctt gatcttcgct gatggttttt tcgccactaa
     1741 ccgatttacc gttaacacta tagacaacag gccaatagga aaaagtgtgg tcataaccag
     1801 tatctttaaa aaattgagct cgttcatttt tacgagcagt aaaataacga tcttcggcta
     1861 gtagataagg gttgccaaga ccgctggcag ttattgaagt acgaataatt tggatagctt
     1921 cactatcata acggctaagg ttgggagggg tggcaggatc aaaataagtg ccaaagaaaa
     1981 aaattaccgg ccgattgttg attttgagat agttgggctg ttttaaataa ttgttgataa
     2041 tgaatggcaa aagatgattg cgaaggtcat ttttgtcgga aaaggtgagg gtttgtggtt
     2101 gatgattggt aagcatgatg gcgaatttca tttgatcttt aaaactagaa cggagaaagg
     2161 atttatggag agaactagtg taggctaatt tttcgagagt aataggttga cctaagacac
     2221 tatcgtcacc agtgcacgta gtggtggtgg cattgctaca acgataccag tcgaagatga
     2281 agaaattgat accgttttcc aaagcccact ttatttccca atccatggct tccggattgt
     2341 cgacgtcaaa atagccgagg aggggttttc gggtcgaatc aaagttttca atacctcgcc
     2401 aactggcggc ggaaatgatt gaatctgatg gtaatcctgg catataaaag gcgccaatat
     2461 aaggaacatt ggtatcggga gaggggagac tgggactggg aaccgcagct ttgatttgtc
     2521 caggaagggc aaaaaagaga gaaaggatca agagccacat aaattaattt tatctaacaa
     2581 aagataaatt agctattctt tttgagccat ttttcccgct ttaaatagag aaaaccaaga
     2641 atgagaaaag cgccgacgac agtgagaata ctttcaagat tacccatccc gttggcaaga
     2701 ccggtaaagg cttcgccaag gttgtagccg ataaagccaa gaattatggc ccgaatgatg
     2761 agagagagaa acataatggg aaaaaatttg gccggtttaa atctaaaacc accggaaaaa
     2821 gcggaaatta gggagactgg aaccacggga atgataacgg caattaaaat aaataactca
     2881 tcggcgatgg ttttttgaaa atcagcgcga actttttcaa tcaatttcca gctgatgcca
     2941 atgtatttcc cgaatttatc aaggaagggc cggccgccat aataggcaat gaagaaaact
     3001 agggcagtac caataacggc accaatagaa taaggaatag aaacgcgagt aataatttgc
     3061 cagaggatat taaaaaattt gtcctgcttt gaaactaaaa aaacaccacc agacatggta
     3121 ataattggtg atgggatggg aacaattatt tgctcgacga taccgccaat aaacatggaa
     3181 agagggccgt gggttttgac catggcgaga attgaattta aaatttcggt catggcttta
     3241 agtataacag gaaattgaag aggaaaaagt ctaaagccta aagagaaaag ggatggtaaa
     3301 atagattata tgggtataga tagtggacat aattttgaaa taatgagtcc tccaagagga
     3361 gtgagagaag aacagttctc aagaggatta acaatccacg tgtggaataa tctttttggt
     3421 aaaggtatgg tacgaggaaa ggtggaaaag atagtaagaa tttatggaaa accagacaag
     3481 gtactaatga cagctaatac aagagtacag tttgttaagc ctgaggatga aaatgggatg
     3541 ataacctgga aaagtcagga tcagctaggg gtggtaacat tagatgtaga taaggccgag
     3601 agaatggtga ctgaaaaaga tccggaaacg ggaaaaaaca gagcaattat ttttaaagag
     3661 gttgggaaac agatcaagtc aggaaatatt agacacgtga tgtggagatt ttccccattg
     3721 aattctaaat agtatttagg tacaattgga gcacttgatg ctaaagctca acttcggaat
     3781 ggaaacaata aataaaaaat ttatcgatga ttaggcggta agtgtccgaa ttgagtcccg
     3841 cgtcggcggg attttttttg gcagttaagg cactttttaa aaacatggcc tcgccgcctt
     3901 tgcaaaatgc tatggcgagc caaaggggcc catagctcag ttggttacag cgcttcattt
     3961 gcaatgaaga gatcgggagt tcgaatctcc ctgggtccac cttcgccann tacggtggat
     4021 aaatccacca actattaaaa atttttagaa ttagggaaag aatctgaatt gattgtttct
     4081 cttgactaag aatagtactt taacaatcta ttgaattgat attttaaatt tatcttggca
     4141 actaaactga gttgcttagg atattaaaga attttattaa agcaaatggt ggatgcctag
     4201 gtggctaaag ccgaagaagg acgtagaaga cggcgatacg cgtcggggag ttgtcaacaa
     4261 atgatgatcc gacgaattcc gaatggagaa atccctgact atgaaagtag tcggaggcta
     4321 gtttcgtaag aaattagccg ggagacccgg ggaagcgaaa catcctagta cccggaggaa
     4381 aagaaatcga aagagattcc gttagtagcg gtgagcgaac acggaagagc ctaaaccgtc
     4441 cttcgcattg tgcttcgcaa aatgcttcgg aacggcatgc ccgcaattaa gtgggcgtag
     4501 ccaatccata gcacctacga agtagagtgc ggaggatggg gttgcagggt ttagtataaa
     4561 gttttgaaaa aacagaagaa agttaaggaa tgaactgcca aagagggtga aagccccgta
     4621 tttgaaattt gagtaaaaac tgactgaata cctaagtacc gcgatccacg tgaaatgtcg
     4681 cgggaatctg gggcgaccaa gctccaaggc taaatacttt agcagaccga tagtgaacta
     4741 gtaccgtgag ggaaaggtga aaanntgatg tcgtgcctat tgaagaatga gcccgggagt
     4801 gtttctagtt atcaagtcta actccgatga atcggaggaa ggcgtagcga aagcgagtgc
     4861 gaatagcgcg aatagatgac tggagacgac ccgaagccac ttgagctaac cttggacagg
     4921 gtgaacgtcg atggaagtcg gcgggaggcc cgtacggatc agagctgcaa atctgtccga
     4981 tgatctgagg ttagaggtaa tatgccaatc gaaagtggga atagctggct ctctccgaaa
     5041 tatatatagg tatagcgtct agtgtttcct ggtgggggta gagctactgg atgagtgttt
     5101 tgtgcgcaag cacaggcatt taaccaaact ccgaataccg tcaggctaag ctaggcagtc
     5161 agtccatccg ggctaagctg gattgggcaa aaggggaaga ccccagactc tcagttaagg
     5221 tctctaaatc tatgttaagt gggaaaggca gtgggttgcc taagacagac aggaggttgg
     5281 ctcagaagca gccanngaaa gcgtaatagc tcactgttcg actccccgta aggggggtcg
     5341 gcaacctgcg ccgaaaatgt aaggaggcta aaacatagta ccgaagctgg agatttgtgc
     5401 cgatttatcg gcacgaatgg taggagagcg ttcttggtgc ggcgaaggtt gaccgaaagg
     5461 tttgctggag cgccaagaag tgagaatccg ggcatgagta acgaannnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnggccg aaaggcgtag gcgatggata accggttaat attccggtac
     5581 tttggtaaat tcgttatcag ttggggcgtg acggtgagtg gaagatttag agcagaagtg
     5641 aaatgctgct tttaagtgtc tagctagaag gggttggcaa atccgccttt tcgttataaa
     5701 gtgaagcacg aataagagtc cgccgtaagg tggacaattg aattgaatcc atttatccaa
     5761 gaaaagcgtc gcaatgagaa tttactgaat tcgtaccaaa accgacactg gtggatgagt
     5821 cgagaagact taggagattg aaagacagat gattaaggaa cttggcaaat taactgggcg
     5881 taagttttac aagatgccct gcccgcccta acaagcgggc tacaacaaaa gaatgcatgg
     5941 cgactgttta ccacaaacac aggtctctgc aaacccgtaa ggggaagtat aggggctgaa
     6001 acctgaccgg tgccagaagg ttaagaagat gggtttttcc gcaaggatct gcctgtttta
     6061 taagccctgg tgaacgtcag cagtaactct aactgttcta aggtagcgaa gtcctttgtc
     6121 gtgtaagtta cgacacgtat gaaaggttca acgactgtgc aactgtctta atcatctatt
     6181 cagtgaaatt gaaagagcgg tgaagatgcc gtttaattac atcaggacaa aaagacccca
     6241 tgaagcttta ctggatcttt acattgataa gaatctaaat attgtcgagc gtaggaggga
     6301 ggccctaggg ccgccaatgg aacacctccc ttgtttattt ttgaatctaa cctattccgc
     6361 tgaatcgcgg aagggaacag tgtatggttg ctagtttgcc tggggcaggc acctgcaaaa
     6421 aagtaacgcg ggtatacaaa ggtcaacttg atccggtagg aaatcggatc ttaagtacaa
     6481 aagcaaaagt tggcttgact gtgagactta caagtcgaat agcactagtt tactagagct
     6541 actccgatgc tagacgatcg gagcagggtc gaaagacggt tttagtgatc ctcgtgacct
     6601 atttggtata ggcacgggct tactggataa aagctactct ggggataaca ggctggtcgc
     6661 atccgatagt tcatattgac gatgcggttc ggcacctcga tgtcgtcttg gcgcatcctg
     6721 gccctgaaga aggggccaag ggttggtctg ttcgcccatt aaagcgctgc gttagatggg
     6781 ttcagagcgt cgcgagacag ctcggtctct atccgatgta atcgttcgtt tcttgaggga
     6841 attcgtctac agtacgagag gacctaggcg agggaaccgc tggtgtgcca gttgtgccgt
     6901 caggcgcatt gctgggtagc tacgttctta ttagataact gctgaaagca tctcaagcag
     6961 gaaactgacc ccgagataag gaaacgtagt cccgtaaggg atgagagatc gccgggagac
     7021 taccggttaa tactctgcgt gtggaagatc cgtaaggatt taagctaagc agtggaaaga
     7081 tcgaagaaat tctttaatat caacagcttt tagtctcgat gtacttcggg attagaagct
     7141 gaataggcaa ctcaatttag aggccaagaa aatagaaaaa tagaaaaagt ctaaagacta
     7201 aattctaaaa aaactattaa aagatttaaa ataaataaaa cttacttttt gagtctcggt
     7261 cgctggagcg aggtggctcc accttttacc attccgaaca gagaagtgaa acgcctcagc
     7321 gctgacaata gtcttcccgc aagggatctt gaagataggt cgcggccggg actgagaaag
     7381 taaattaaat tcaatagatt gcaatattta aaaatagaaa aagtctaaag tctaaattct
     7441 aaaaaaaaga ctgaagaaat attattaatt tatttagggt agacaatccc gtttatcccc
     7501 ggtaaatcgg ggattttttt taacaaaatg aaaaaggaaa agaaagtttc taaaaagaaa
     7561 ataacagacc ccatgagcat ggaagaattg ctaaaactag aaggagtggc ggttgccggt
     7621 ttaaagaagg gacaggaggt aaaaggaaaa attaccgcga taaaaaataa atcaattttt
     7681 attgatattg gtggaaaaac tgatgcggtg gtaatgggta aagaatttga atcagtcaaa
     7741 gattatgtag ctgatctgaa agtcggtgat gaaattgagg tccaggtaaa gcaaccggaa
     7801 aacgataggg gtcagatttt agtctcgatt aggggcgcgg cttcaggtta cggctggaac
     7861 tattttaccg aaaaagaaaa aagtggaggc gaagtgacag tttttggtaa agaactaaat
     7921 cgaggtggaa ttgtggtgat tgcccccttt ggtttttttg gttttatccc cggatcccaa
     7981 attgggagta aatatgacgt tgatcctgag aaactgctcg gtaaaaaaat taaagccaag
     8041 gttctggaag tggatcagtc taaaaataga ctggtatttt ctgagagact ggtgtctgaa
     8101 ccgaataagg tcggtgaaga gtcggtggcg attgagaatt taaaactcga cgatgttttt
     8161 gaagcggaag tagtcagggt cgaacctttt gggatttttg tcaaagttga tgatagtcat
     8221 gaaggaaagg cgatgaagct agagggtcta gttcatattt ccgaaatttc ctgggaaaaa
     8281 gttaacgatg tcacgggcat gtttaagcct aaagataaaa taaaagttaa actggttaac
     8341 aaatctgagg gcagactgca attttctttg aaaagacttc aggaagatcc ttggacaaaa
     8401 attgaggaga aatatcctaa ggataaagag tttgatgggg aaattgtgaa aattgccaat
     8461 tttggggctc tcgtcagact cgaggctgga attgaaggtc tgattcatat ttctaaacta
     8521 gctggagtgg ttctcaaaga tggagaaaag gttcaggttt atatcgagtc gattgatgta
     8581 gctaagagaa aaatatccct agggcttgtc aatactgata agagtaaggt gatatataaa
     8641 tagaaaaagc ctaaaattta aaataaaacc cgctttgaat gaggcgggtt tttagtgaat
     8701 aaataaaatt aattttgtgg tgttacttgc aaagatcaaa agagaggttc atccgatagg
     8761 atgatttctt ttggctcttt atcagatgca agttattagg atggttttgg ccgtcccttg
     8821 ggcgaaaaag atgtccaagg gacggcgtgg gggttatggg gtaaacagaa agcggatttt
     8881 gcaggtgggc tcgtttgtgg tttcgcgaaa gcgaagttcg ttgaaactgt caggaaccca
     8941 ggtgaaagta gtcactggtg tattggtttc ttcaagaaaa atttcttcag aagaaagaaa
     9001 cactagttcg tcgacccgaa gatctccgaa tgtcaaaacg gtgcctgcgg gggaggtcaa
     9061 ctgatagatt gttgaaaaaa caaccacatg attgacgatc cccttgacga gaattcttgg
     9121 gcaggtgagc tgggcggtgg ggtctggctt gtcggtgggc tgaatgggct ggttggtggg
     9181 ctgcggggtg actggggtga gctggacagg ggtagcggtt ggttgcggca gagtaggaca
     9241 agtcccttca ggtacttttt cccatggcca atgaaggctg tggcttggaa tccatttgca
     9301 tttgcccgtg acccggtgct gagtccatac acaggtaatg ttggaattac ttccctgtgg
     9361 aggacattct tcttcgggtc tgcctgtggc ctcaacattc tgtttagggg cgaaaacacc
     9421 catggttact ataaatagta gaccaattac aacaagaatt tctaattttt ttttcatttt
     9481 ttctcctatt ccaaaaaatt ttttccttga taatcaaggc tcaaaactga aggtaaaaaa
     9541 ttacctgcaa gtttaagcct tgaaaccagg attaaggaga acggtttacc atcctatttg
     9601 ttaaagaaca atcaagagct gatttaaatt aaaaacacaa ttgaacgctg agttcaatgc
     9661 ggttctaatt gtcgtgtcct atactatagg acattagaaa ttgcaaatta tttgttattt
     9721 tttatattta attttttagt tttaaatttt tgttataaat gcatcaatga tcatagctaa
     9781 aagcgtttta gagagagacg agctagggag tttgatataa tagtaatatg gaaaagaaga
     9841 aaatagttcc caaaaaagaa attgaagtca aagaagaaag cacttatttg agatatgatg
     9901 ttaaaacttt gattacggtg tttttactgg taaccgttta tccggtggga gtagtgatga
     9961 tgttcgtgtg gatgagatgg agtaagtggg ttaagttttt gatagttttg ccggtgattt
    10021 tggcggtgat aattccactt tttattttac tggcggtggg gacagcggtg gtgcgaggag
    10081 gaggggaatt tatcaattcg gatgaattta gggagataag gcggggaagg ataaatcgat
    10141 gtcctaggat gcaaaaagag atttatcgac aacaaattcc aacagtggtt gaaataaaac
    10201 cattagatag gtaaaaaaga taatggtctc atgagaggcg tttaaaacga agaaaaggaa
    10261 taaaggctgg taaaattagc ttatgaaaaa ggtttctgag agtatttttg tttgtgcgaa
    10321 ttgcgggaat gaattttcaa aatggagcgg gcagtgtagc gcctgtcatg agtggaatag
    10381 cttaaaagaa ataaaaagtc taaaatctaa aaatctaaat tctaaaaaaa gaagagggca
    10441 agagacggaa ataaaaaatt taggaaaaat ccaatctacc cccaacccct cctttgacaa
    10501 ggaggggaga taccagaata tattttcaac ggaaattaag gaatttgata gagtattggg
    10561 taaaggaatt gtgcggggac aggtgatcct ttttgcgggt gaacccggga ttggtaaaag
    10621 tactttacta acccaattga ttggtaaaat aggaggactt tatgtagcag cagaggagac
    10681 ggcagaacaa atagccctaa gggtggagcg tttaggtttg gataagaata aatttgatat
    10741 tttagaaaca aatgtagtcg aggaaattga agagataatt gaaagaacaa ataatgtgtt
    10801 caaaatagtg gtggttgata gtatccagat ggtgtcctgc gctgaaagct ctggaacacc
    10861 tggatctctg gcccaaataa gagaatcaac ttttcggcta gtagaaatgg cgaagaaaag
    10921 tggggtagcg atttttattg tgggacacgt gactaaagag ggagatattg ctggaccaaa
    10981 aatgctggag catatggttg atacggtgtt gtattttgag ggagagaaag acggagagct
    11041 gagaattctc cgagtaaata aaaatcgctt tgggccgacg gatgaagtgg gggtgtttaa
    11101 aatggaggaa aaaggtcttc gagaaatatc ttcagaagag gtgagtctaa ttgatagtaa
    11161 aatgcctcaa atcgggtcaa ctataagtgt ggtgatggag gggagtcggc cactactcgt
    11221 cgaaatacaa gccctggtta cagaatcttt ttcccagata ccaaaaagga tattttcggg
    11281 gattgattat aaccggggac agctgctggt agcagtggcg caaaaagtgc tttcgatacc
    11341 actttataag gaagatgtgt ttgtggcggt gtcgggagga ataaagattt ctgatcctgg
    11401 agtagatttg gcggtaattg gagcgatata ttcgagttat aaagataaaa taataatggg
    11461 agagggcgac cataaagggt cgcccgtact aattggcgag gtgtcgctgc tcggaaaggt
    11521 gagaaaggta agaatggcag agagaagaga aagggagata aagagtcttg gtatggaaat
    11581 gatgaagata gagaacattg gagaattgaa gaaagtctaa tctacaatct tcaatcttca
    11641 atctacaatc ttcaatctaa agagtaattg tagtttgata attgaagatt aattaagttt
    11701 tccacatagt gttgataagg ggggagttgg gctgaaaatg aagctaaaag tattaataaa
    11761 gataactata agataggcgt ttttcgtgaa gtaatttctt atagggtata gacaagaaaa
    11821 aaagatggtg ataacatctg tcaatgtcta ggaagtggtg gaagaagtta tcaaagaaag
    11881 ataagtgggc ggagaatcag atcaaggcga tggcacgact cggagggaag ttggtactgt
    11941 aaaattaaaa atttgagaat atggaaatac accgataaat cggtgtcttg tctttaggta
    12001 aaatttggcc ctagcggttc aaatttcacc taaagaaaag aaaaaagaaa caactttatc
    12061 gatattctca aagcaggtgg aacacgaata ttgttgtttc ttcaatttct aaaataaagg
    12121 aaaagagccc aattgtcaaa ttggcagatt tgaattgata ttagtattga gagcggtgga
    12181 gggggcaggt ccgccgcttt caatagtgat attaatttga cagtaaggtg agaattttat
    12241 acaataaagc attcccagtg tagaagtgtg ttgatgttta tatatttttc ccaataatgc
    12301 ctagaaaatc agtaaaaacc caagaggtcg tggcggtgcc cgccgacgca aacagctctg
    12361 gtgagataaa atctgccgaa gttaaaacag agacaaacaa tcgtgtggtg attgttaatc
    12421 ccaaaagaga ccaggcgatt atgtcgccaa agatcgacaa cagcctggtg aataaaaaaa
    12481 tagaagtaga aaataatcca atgagactcg aaataatttc gggaattctc gagataatgc
    12541 ccgatggtca gggctttgtc agaccaaaat ttaatcccgg acataaagat gcagtgatcg
    12601 gctcgatgca aatcagacgt tatagcctcc gagtgggcga tttaattacc ggggtagcca
    12661 aggcgccgaa acaaaatgaa aaatactgga gtctggtccg aatcgataaa atcaatgggc
    12721 tggacctttt agcggctcaa aacagagccg aatttaaaga tcttgttcct atttatccgg
    12781 taaagcaggt aaaacttgag tttgacccaa aaatgttttc aacgagaatt atagatattt
    12841 tttgtccgat tggttttggt caaaggggga tgattgtatc gccgccaaaa gcagggaaaa
    12901 cgaccctgtt aaaagaaatt gcggcgggag tagcagctaa ccacccagac gtccacttga
    12961 tggcggtgct ggttggagag aggccggaag aagtaactga catgcaaagg tttgtcaaag
    13021 gagaagtgat tgcctcgcat tttgaccaaa aacctgaaga acaaaccaga gcagcggaaa
    13081 tcgctatcga aagagccaaa agactggtag aaatgggcaa agatgtcttt atggtttttg
    13141 attcgattac cagactagcc cgggcatata atctagccat tcccacctca ggtaggactc
    13201 tgtcgggggg gtttgatcca gcggccttgt atccgtcgaa aaaattcttt ggcgcggcca
    13261 gaaaaattga aaatgggggt tcgctgacga ttattgggac ggccctggtt gatactggtt
    13321 caaaaatgga tgaactaatt tttgaagagt ttaagggaac aggaaatatg gaactgcgcc
    13381 tcgagagaaa actagccgaa agaaggatat ttccggcttt tgatattttg aaatcaggaa
    13441 caagaaagga agaattgctg ctaccggtag aaaccttgtc gagagtcatg gcgatgagaa
    13501 gaatgtttga taatcttgag gaaaaggatc aaatgacgga actgattatt gaccagatga
    13561 ataaaactga aaccaatgaa gattttttga agaaagtagg gaagagataa gccaatgaaa
    13621 gaagaaattc aactgataaa acaatttttg gaactgcaag gacgcttttg gacggagata
    13681 ggtaatttat ttcttggctg ggtggtcaag attaaagatg gtaccgccaa gctaatgtat
    13741 aaacaaagag ggcgtttttc ccaaagtttt gtcagtatca gtctggctgt tttgtctttt
    13801 ggaggaatta ttttttcggg caatctcgaa gatttaatca gaaatagaga taatagtgaa
    13861 aatagaggaa gtggctacct gattatggcg gcggataatg aagagggagg agtagaaacc
    13921 ttgatttcta atttgcccaa aggggagctg gtggagtatc gagtggttga gggagataca
    13981 atttctagta ttgcccaaaa atatggagtt agtattgata cgattctgtg ggaaaataat
    14041 ttgaagtcgg tcgaagcaat taaaccgaac cagattataa aaattttgcc ggtaactggg
    14101 gtaaggcata cggtaaaacg gggagaaaca atttatagta ttgcaaaata ttatggagtt
    14161 gatgcccaaa atattattga ttaccctttt aattctttta gtaatgatga aacctttgcc
    14221 ctggtcgccg gacaagagct gattgtaccg gagggaatta aaccaaaaga aataattacc
    14281 gggacaaaat atatagctag gacagtggcg ccaattcccg gagtggtggg agagggtaat
    14341 tttatgtggc caacatcagg agtaattact caaagatatt cttggtatca tccggcggtg
    14401 gatattgcta atgccagtaa tccgccagtg gtagcggcgg cgacaggatc ggtagtgcag
    14461 gctggctgga atgggggcgg ctatggatat tatgtggttg tagatcatgg taacggattc
    14521 caaactcttt atgctcatct tttaaataat tcaattgtag taaaggcggg agacaaggtg
    14581 tctcaggggc aaaaattggg aacgatggga agcacgggca gatcgacagg aactcatttg
    14641 cattttgaaa taaaaagtgg
//
LOCUS       DHGB01000048           17265 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_92777, whole genome shotgun sequence.
ACCESSION   DHGB01000048 DHGB01000000
VERSION     DHGB01000048.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17265)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_92777"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         4754 a         3568 c         2664 g         6277 t
ORIGIN      
        1 tatcctgtcg ctaaatattt ttttacccat ttattgtttt tcaaaaattt ataatagata
       61 ttattctttt cccaaaccgg tttcatcaaa attaaatcgg tggcattttt taagtttctt
      121 ttgtttttgg ggatgctgag attattttca tcgagccaca aattaaaaca aaattcatca
      181 gcattttctt tttccccaaa ttttcgatga ggaattttat gccaccaaat aaaaaatctt
      241 agatataatc gccaccacca tagttcatta tttttggtca ctattaaaag gtcaatatct
      301 tcatttttaa aggcaaattc cgcggccacc gatccagtca ctcctaccat taaaatacct
      361 tccatttttc ccaaatattt ttgagtaaac aattctgctt tctttaattt attttgccag
      421 tcattttggg ttatcttttt atttcctttc cccattatct cagtcaatcg atagctcttt
      481 tgtgaaatta atctcaaaaa caactgctgg tcattaagac tttgtccgta tttttttgca
      541 taatttcgtg ttttattaat tgctgttttt agctcgttca ttaacgtatt ttaccctctt
      601 attgttataa tacctaagtc tcaaatgaat aaaaatacta gaagatatcg cttttatgaa
      661 atgatccccg gcatttttgc ttggtttgtg attctttttc ctatctgggg atcattctta
      721 ttgccaaaag tcgtcgctta ctttgtcctg gcttttttag tttactggct ttacaagtca
      781 tttaaatccg ctattttagc cgtttttggc tattttaaaa ttcaaaaagc caaaaataca
      841 aattggcttg aaaaatttaa acaggatttt cgggcttcct ggctacacta tcaagatatt
      901 aatcacgttg tcattatttc ctcctacaaa gagcccctcg agattattga aatggctttt
      961 tctagtnnca agaagcccga gccggggaag aaaataatca aaaaacaatt aattattttc
     1021 aaaaaaaata taaatcagtt tttggatcat taacttttac tgaacatccc agcaatttac
     1081 ccggcgaaat tgccggtaaa cacaccaatg aagcctgggc cgctaaatat ttcaaaaagc
     1141 attatcttga taatcctaat tgtagattga agattgaaaa ttgtacctta acttcttgtg
     1201 atgttgatac tatttttaac cccaaatatt tttctgccct gacctataat tttgcctcaa
     1261 atccaaaccg ttattttcgc ttttggcaat cacccatttt ttggcaccac aatattaata
     1321 gtgtccccgc ctttattaga atcaccggaa ctatgggtaa tgccattcac attgccaata
     1381 ttcaggagcc cgatggcatt ttctttaact attcctgcta ttcttcttcc tacaaattaa
     1441 ttgattcggt tggctattgg gatcccgata tgattcccga agattggcac atttttttac
     1501 agaccttttt tgccactggt ggaaaaacct tagtcaatcc tatttttttg ccaactattg
     1561 ttgatgctcc agacggaaaa aattattttt cggctctaaa aaatcgctac aatcagtgtc
     1621 ttcgtcatgc ctggggggca attgatattc cttatgctat tgagcagtct cgtcttcacc
     1681 ctgagattcc ctggctaacg agggctctcc gcctctataa actagttcaa acccatttta
     1741 tctggtccag caactggttt attttgactc ttgggacttc tttgcccgtt gcccttaacc
     1801 cttatttttt caatactact cttggttaca accttcctag aattgctaat cttattttga
     1861 ctatttgtct tattccgatg tttattctca ttgttctcga ttggaaactt cgcccagcct
     1921 atcaaaaaaa gggtttttgg aacattctta aaaatattct tcagtggcca atgatgccta
     1981 tcgccactct ggccatgtca gtcttgcccg gacttcattc ccatacccga cttctatttg
     2041 gtcgccgtct tgaatataag acgactgtca agacctctag tgtaaaataa agataatatg
     2101 ttttacgcca ttctattttt tcttctccgt ttaccttcac tctttgaacc ctattggtat
     2161 ggcgacgagg gaatatattt agccatagga caggctcttc gccacggtct attgttattt
     2221 cagcaaatac atgacaataa acccccaact ctttattatc tggccgcctt gacacatacg
     2281 gtttttggtt ttcgcttact cttgttttta gtcatgattc ctactattta tttcttttat
     2341 cgattagcta aaaatttttt atccccgaga cttgcccagt attcaactct ccttttttta
     2401 gttcttactt ccatccctct ttttgagggt aatatcgcca atgctgaagt tttcatgctc
     2461 cttcccacca ttcttgccgt ttactttctt tataaatatt cctcagtttc tagcttgccc
     2521 cccaaagctt tagcgtcagc gggtcttctg cttggtctgg cttttaccat caaagttccc
     2581 gtagcgattg aatttgcttt tctttgtctt tggcttttaa ttttttacag atcaaaactt
     2641 aaaaatcttt ttattttttc tttttctttt ttagttccga ttactcttta tttaatctat
     2701 tttgctctta agggtgctct ccagcctttt ctttttgccg cgttacttca aaattttggc
     2761 tatctctctt cctggtcaac cgggaatcaa caagcctctg ttgcctccag tggcttaatt
     2821 aatcggggga tagttctttt aatactttgg cttatttcat ttcttctttt tcatttcaaa
     2881 aaaatttcta aaaacatttt ttttctttca tcttggtttt ttgccgctct ttttggggct
     2941 ttactttccg gccggcctta tcctcattat ttaattcagg ttttacctcc tctttgtctt
     3001 ctcctatttt tctttttgaa atcaaaatta tgggttcttt ttgttttttc tctctttact
     3061 gtttctcttc ttcgctataa attttatttt tatcctgtat taccatatta tcttaacttt
     3121 tatagttatg ctcttggcaa aaaaaacact gataattacc ggcagtattt tggttatgac
     3181 atgtctcaaa attatctcct ggctagtaaa ataaaatccc tgacgagtcc gagtgacaaa
     3241 atttacgttt ggggagatca gtcttatctc tttcccctaa gtgatcggct tccagccacc
     3301 aagtatctcg tttcttatca tgttgtcgat tttagagctc acgacctgac catttcccag
     3361 cttaaattaa ctactcccaa attaataatt tattatcctc agccttcacg cccctttccg
     3421 cagcttgatg attttatcgc taattattat gttcttattg accagattgg ttccgcttac
     3481 ctttttaaat ttcgagacta aatcatgaat gttttttctt ctttttttgg taatttaggt
     3541 aagttttggc agggtagtct gaactcccgt tttttccgtt atagtctatt tttgttccta
     3601 gcccaaatag ttataattac ttggttttat aatcaattac ctcctgaaat tccaatttat
     3661 ttttcccgcc cttggggcca atcctggtta tcctcttcag cttcaatttt tattcttccc
     3721 ttattctcct taattacttt acttataaat tattttctgg ctctttattt ttacaccaaa
     3781 aaaattctcc tctctcaact tttagtgatt tttggattta ttatttctct cttttctatc
     3841 atttcacttt tacagattat aagcctggca atctaatgaa ttttctttat ttactggctt
     3901 tacctaccgc ttttttaatt tcttatttta ttaccccttt ggtaattaaa tttttccatg
     3961 ctcatcattg gatcgaaaat ccaaaaatca aacaactaaa aagtggtaat gccacggcta
     4021 cgaggcctgt tccgagaggt ggtggcctac caatctttat cagtgttttt ataacttctt
     4081 taatcttttt gcccttagat cagcatttaa ttggtatttt aatggcctca ttttttgcct
     4141 taataattgg ccttttcgac gatttaaaag acataagtcc tctttttcgt ctctttacta
     4201 attttatttc tgccatgatt gtggtggcgg ctggcattgg cattgcctat gtttcaaatc
     4261 cctttggtgg tgtttttgat ttatctcacc cccaaatctt tttttccttt atgggaggga
     4321 ctcattcaat ctggcttcta gctgatctat tagccatctt ttggattgtc tggtgtatga
     4381 attttgttgg ctggtcctcc ggggtagatg gtcagcttcc tggctttatt tctatttctg
     4441 ctatttttat tggtattctt ggtctaaaat ttcaaaatga tgtgactcag tggccagtaa
     4501 ttattctggc tttcaccgtt gccggtgctt atctgggttt tttacctttc aatttttatc
     4561 ctcaaaaaat catgcctggt tatagtggca aatctctagc tgggctgtta ctggccattc
     4621 tttctatttt atcgggagcc aagcttgcca cagttatttt tttacttggt ttgcccatgc
     4681 ttgacgctat ctttgttatt gtttaccgtc tttggcatca tcactctcct tttaagtctg
     4741 atagtaatca tcttcaccac cagctattaa agctcggctg ggggagacgg cggatttctt
     4801 ttttctattg gtttctcagt ctggcattag gttttttgtc cctgatttta aacagtcagc
     4861 aaaaatttta tgtttttatt ggtcttgcga ttatcttttt tggttttact cttaaatttt
     4921 ttcggcgtat ttaaccgaga tccacccgct tttatcatca aatttaattt gataccaatc
     4981 gttattttga cttagtagtt cgtagctttc tcctggtttg acccggccta tctctgctcc
     5041 actaccactc gcattacttc tcactcttag ccatccagtt tctgtttctt ttattttaac
     5101 catagggacc atttgtttaa gtggagttgg acttgagact tcacttattt ttgttttttc
     5161 ctctttttct agcttcgcgt caattactat ttggtagttt ttaaccattt taacgattaa
     5221 attaacactt ttaaaaccgg gataactcag tgaaattttt ttatcaccct ctcccaaatt
     5281 ttcaatttta tttggactga ttgcttttaa ttctccatcg acaaaaatca ttaccccacc
     5341 aggaattgaa ttgataagta aaccatcact ttcgccgctt ttttccattg ataaaatata
     5401 accactacta ttatcttcac taagcttcca attaattacc gtcgtcacat tattttccag
     5461 tcttatctct ctttcccagc tattgccctc atcttctagt ttaattgtaa tttttcctgg
     5521 tactagagaa ttatttttat aaggcgtcat tccgctttct ttcccgttaa gatatacttt
     5581 agctatgggc gagctcacaa tttccactcc agatttttca accccacaag cacccagtaa
     5641 taaagcactg aataatacaa taactaaact gatatttatt ttttttctaa tcattttctc
     5701 tctcctatta tattttattt ttgctcctaa aactcttata aatcccattg tcccgccgga
     5761 gcctaatagc gaaggcggat ctacccctac tcctacgtca attaccacca ttactctcgg
     5821 tctagtcggt gatctaggtc ttggccgtca tattacttca accgctcgtc agaaaaacga
     5881 tttttcttgg tcttttaaac agatatctcc ttggttatta tcaaatgatt ttaacctggc
     5941 aaatttagaa agtcccatta ttgctccttg ccctgaaggc aaaacaggga ctttcacttt
     6001 ttgtggcgac gaaaagttct taccttatct taagtcaaat aaatttgttt taaatttagc
     6061 caataatcac attttaaatt atggcgaaga tggcttatct caaacccata actttttatc
     6121 agaaaataat attatttatt tcccggaatt tgcaactaaa aaaattaatg gcattagctt
     6181 tggttttttg ggttacgatt ttattaccta tcctgtcatt gcgagcgaag cgcggcaatc
     6241 ccaaataatt gctgatgttg agagatataa tccggaggtt gactggctca ttgtttctat
     6301 ccactggggt aacgaatatt taccctcggc tgagagctgg cggataaatt tagctagaga
     6361 gttggttgat gctggtgccg atattattca tggtcatcat ccccatgtct ggcaagatta
     6421 tgaaatttat aaagataaac ccatctttta ttcttttggt aattttatct ttgatcagtc
     6481 atggtcctac ggaacttctc attcccagat tgcccggctt attctttcca aaacaaaaat
     6541 taaaaatctc gagctttttc ctattgaaat caagttcaac tcccagcccg ttttaatgcc
     6601 ttaaatacat tttggctggt attcaccctt attatttagt tcgagaattc cttcccaggt
     6661 gaaaaatttt tccgatagtg gcctaattct taaactttta caattattta tcagtcccag
     6721 ctccttttgg ctatcgacaa ctaaaatggc caccagtatt atcaaaaaaa cgataattcc
     6781 cgaccaccag tacctatttt ttttatccat tttttaccgc tttaattgct aaaaacagag
     6841 ggaactgggc cctcgctcta tcttctatta tagccattgg tccctcagat ttctttggtg
     6901 aaatccactc ctccaaattt tttattaaaa atccattatc ggaaagcatc tccacatatg
     6961 ccgacaaggg atagtgataa ctataagaaa tttgattgtc ttttttatca aaagggctgg
     7021 attctatggg aatttccagt ggtgataaat agctctccat tatccgaaat tgcttattat
     7081 cttttacttc ccagtcgcta tgtttgggga ttctaaaagc tggatgatta ataacgatca
     7141 atagttctcc atttttgttt aataattttg aaaaattcct aattactcca aaaggttttt
     7201 tcacattttg gagagctaaa ataatcattc ccttattaaa actaccgtaa atttttaatt
     7261 ctttagttat atcttcaacc agataagtgt ggttaatatt tttgtctagt tttttggctt
     7321 cctcaattaa ttctgtcgat aaatcaattc ccagatattt atttttaatt tgtcttccca
     7381 ttataccttg gccacagcca agatccagta ttttttcccc agcttttatc tcgagtagtc
     7441 gcagtgaatt tggaatgatt acttcctgat gataataact accttcaacc ccgacgcttt
     7501 ttttgtacca ctgcgccacc ttgtccttcc aggcagtttt atgaatttct tttttccacc
     7561 tcatcccagt taattatttt ccaaaaatta tcaacaaatg tttttctgtc actgagataa
     7621 tctagataat aagcatgttc ccagacatct aatactagta ttggtttcat tccatttaaa
     7681 cccaataaat tgtgtttttc caattgtcca atcgttaaat tattctcttc atctttccac
     7741 agtaccgccc agcccgatcc ttccacgctc accgccgcct ggctaaattc tttttgaaaa
     7801 ttttcccagt tgccaaaatt tgaatttatg gctgccaaaa tattatcagc tggtttatta
     7861 ttttcatctg cctttcttaa attagcaaaa aacaattcgt gtaatttggc gccgttatag
     7921 ttaaaactaa aatctctcat tacttctcgg tttttttctg gtttttctaa agccatatta
     7981 gctccgttga cgtaagccac ataatgttta tcgtggtgta gctccattat ttttctcgaa
     8041 attaccggct caagttcttc acaggcatag gggaggggga ttatgtttag tgttttcata
     8101 gttaactgat tatataatag taatctattt ttcattaggt gataaaatta catatgtcgg
     8161 aaataaaaat tatttcagct tctttgaatc aaattaaaga gcttcagaac ctaaatgatg
     8221 aagtttttat tgacaatagc cgctatgatg aagatctaaa aattgactgg gctcaatcag
     8281 acgagggtgg aaaaaaatat tttactgaat tattaaaaag tagtaactct atttgtttaa
     8341 ttgctaaaga taatgataaa aatatcggtt atttggttgc ctgtcctaaa gaaattcctt
     8401 atcgtttaag taaatacatt gaaatagaaa atatgggtgt aattccagaa tatcgctgtc
     8461 ggggcattgg tcaaaaatta attgatgagt gtttaaaaat cgccaaagaa agaggttttc
     8521 aaaaagtata tgtaaatgct tattttaaga atatcggtgc catcaatttt tataaaaaaa
     8581 acggatttac agaaatcgat ctcagtttag aaaaaagtat taaataatac ggctaccaaa
     8641 aaattcacca tcctccacag taatgatatg cactgggatt ttttagcccc agacgtcatg
     8701 gtcaagtatc ttcgttctca ccagaatcta atttctaaag ttgagggtcg tcttatttat
     8761 cgctaagttt tttctttttt ggccagatca aaataacaat aattaaaagc aataatccaa
     8821 ctgtaatttg ccaaaaccag ttacttaaat cctttgtatt ttcggcttcc tcggttgcct
     8881 tttttaccac ttcttcagct ttcgtcggct ctttacttgg ttctattgtg ggactaattt
     8941 cggttttttt agtcggctcc aatgttgctg ttactacctg agtaaccact acttgttttg
     9001 gtgtcggtgt cgcggtcgaa accagttgaa acttaatatc ttccgggtca atgtcttttt
     9061 taaaaatcaa ggttggtatt ggtgtcggtg tcgctgttgg cattatctta aattctctct
     9121 caataggaag aaaatctaga gccattattc ttgaaacatt aaaaataaaa aaaattccca
     9181 aacacaaaac tgctttgatg tatttcattg catacatttt acctcttttg tcaaatataa
     9241 atcaaaaacg cggtataatt ggctgtgatt ttaatcaaaa ccccatccca aattgatggc
     9301 attagagctt cttcaaaact tgccactgcc actctaaaat acactgcctc ttttattaaa
     9361 agcggtattt ctactgaatt tttaaatcaa aaagctcacg attatataat cgctcataaa
     9421 gccattcccg cgcctctcaa ttatggtggt tttcccaaaa gtatttgcac ctcaattaat
     9481 aatgttgttt gtcacggtat tccttcaaaa aaggaaattc taaaagaagg ggatattgtt
     9541 aatatcgatg ttaccaccat tttaaatggt tattatggtg acacttcagc cacctatcca
     9601 gtgggaaaaa tttcttctga ggcaaaattg ctaatcgaga ggactaaaaa atcactcgac
     9661 cttgccatca aatcggttaa agccggccag tatcttaatg agagtgttgg tagggtcatt
     9721 gagaattatc ttaccccttt tggttattct atcgtccgtg atctgggtgg tcacggtgtc
     9781 ggtcttaagt ttcatgaaga cccttttgtt tttcatcaca atactccgga gaatcatatt
     9841 ttgctaaaag cgggcatgat ttttactatt gaaccaatgg ttaatgcttc tcccaattgg
     9901 catatttatc aggacaaaaa tgatggctgg accatccaga cggaagatgg ttctctttcg
     9961 gcccagtttg aacatactat cctggttact gattccggag cggaggtact aactaaatta
    10021 tgattcttct cgatggcaaa tctctctcgg aaaaaattct ctccaatcta aaattgaaga
    10081 ttgaagattg tagactgaag atcaatttag acattgtttt agtcggggat aacccttctt
    10141 ctttaaaata catttccctc aaacaaaaaa gagccaatga ggtcggtatt ggtggtcaac
    10201 tatatcatct tcctgatact atttctcaaa aacaaatttt cgaactattc aataaattaa
    10261 ataaagacta tgataccacc gcttatttca ttcaattacc agtccccaat attcgtcaaa
    10321 tttcttcact tttgaataat ataagtattc acaaagatgc tgatggttta aaccccaatt
    10381 cgggggttac tccagcggtg gtcaggggaa ttatttctct tttgaaacac tatcaaattt
    10441 cttttgataa ccaaaatatt gtcattgtta atgactcaaa tttaattggc cagcctctta
    10501 aaaaatattt ttctgatttt acttctcaaa tttttttact caatgatcaa acaacaaatc
    10561 ttacttcttt tacaaaaaaa gctgatattc taatctccgc caccagtgta aaaaatttaa
    10621 ttacagcgga tatggtcaaa gatagtgtca tcgcaattga tgtcggtggg ggagatattg
    10681 attttgataa cgtttctaaa aaatctacct atatcacccc tacttttggt ggtgttggcc
    10741 ccatgaccgt cgcctctctt ctccaaaaca cttacgatct tgctacaaaa taataatggt
    10801 atatcattgt cctgttcctc ttctatagac tgccgcccag ggaacatagc tactggtaaa
    10861 agttttttta tgaattattt ctccgtttct ggttacggtc caatcaaacg ccgtttttaa
    10921 tccgggtact cggctttcat ccttaactac tttacccatg ggcagggttg gatcgtcaat
    10981 ccacacatct ggtggtgctg gtgccgcccc ccactgttta taattatcaa ttactgtttt
    11041 tctgccatct gaagtcccgt aaatctcata agttaatttt ttatttttac catcataata
    11101 actttgaatt agaaggtgat gtcccgtatc gttgacaaag gctaaatctg gtttagggat
    11161 aaagactgtc gcatcaaaac ctggcttgga gtcttcttca taataggaaa cccggtaagc
    11221 atgaggctga cgggcagtaa tgtcaactcc ggcatcaagc atggctctaa ataaagtcgt
    11281 tgaaacttga caaattccac caccaacatc cagttctgtt tttccctgtc tgataatata
    11341 tgccttttta tagccattat cgagacttac ctctccgaga gatttaataa aagaaaaagt
    11401 ttctcccggt gccaccagta cccggttcac aattgaagct cctttttcca cgttccagtt
    11461 tctgatttcg ctactatgtt taaaactgga agtccctctt cccagcagtt ctttaatccc
    11521 taggctattg acatcagaat tatttatttt tggttgaacc ttaactagtg gcagtccgct
    11581 gattatattt ttttcacttg aatttctcca gtttaaaaaa tcacgacata tctccgtttt
    11641 cattttttcc gtatctatcg aatatccctc tttggcaggg agaaaatcaa ttacctttcc
    11701 attctcaaat ttaaaaaccg catcttgtgg ctctttgttt aagctttggc ttaaattatc
    11761 aacaaactca cttacttttt gagattgaca gccaccccca aaactaatcc agttgacgag
    11821 gaccttgtcg tcaatttcgg tacttacttc tccatcttta aaaatcactg attttccgac
    11881 atagtttttt gccatttcta gcgcctcttc cttatctttg tcacttggta tttctccaat
    11941 tatttcgact ggtatttgaa cctcactgtc tatttcatat tttttcaact tgctgatgat
    12001 aatctcttta agaagctctt ggttggtact tagtccaagc tctccttcga ttatctcaat
    12061 ctttttatcc aaaactactt cagccggaat aaaaggttta tcaatctgag atgcaatttc
    12121 cactaccatt ttgtttagtt tttcctggtc aaaatcaact tccagttcaa aatctttact
    12181 ctgaaaaaag gctttaatat atttgccaat tccttttttt aggcggcgaa aaagcaaatc
    12241 agatgccatc tgatcggtgt caatttttgc cgaaatagaa gccaaattaa ccgtaaagat
    12301 tctttctccc tctctaaaat ttaaatttcc ctccatggga aaatcagaat tcaacttatt
    12361 gattatttcc ttttttccca aaagcgaata gttttttccc aaaaccgtcg tttctttgcc
    12421 tgttttgcca aaaccaaacc caagtggtgc cagaaaacta gctagaatca aaaataaaat
    12481 actaataatg attaaaattt tatttttcat ctgttatcca tcgctttgtt agctaaagca
    12541 tctgctcggg aattggattc tcgttttatt tctttataaa taattttttt attcttagat
    12601 aaattttgcg caattaacca tagttttttt aaattttctg ccttcacttt atattttcct
    12661 tgcatttgtc gtaccagtaa ctgtgaatca gaattaattt ctatctctga ttcaattttg
    12721 ttttcttcga tccaagttaa cgccccaatt aaacccaaat attctgcctc attgtttgtt
    12781 tttactccca aaaattttgc ttcttcaaaa agagtttttt tattttcatc ttcaatataa
    12841 attccgtagc cagctattcc cggattaccc cgcgcgcccc catcagtaaa cacctttatt
    12901 cccatgtaag tatcatagca aaaaatacat tattcccaaa cctatgagca ccgaaatcat
    12961 ttgccataaa ctctgtttaa ttttattttc atgtcgatga agctcaggga tgagatcaga
    13021 ggcggctaga tagataaagc caccagcggt tatggccatt agctctcctc gtattttacc
    13081 gatatcactg ataagccaga taactaagac tccaagaaca ctcagaaagg cagagcccag
    13141 gttcattaaa gctgcttttt tagctgaaaa tcctccatga atcaatattg caaaatcacc
    13201 tatctcctgg ggaatttcat gcataaatac tgcgagggaa gtgcttattc caattggaat
    13261 actaatcata aagctactcc caattattag tccgtcgatt aaattatgaa ttgaatcagc
    13321 cagaagactg atccacacca gctccttgtt ttgattacaa tcgatatcgt gacagtgcct
    13381 ccattttaat attttttcca gagaaaaaaa gataagcatc ccggtgataa tcattagtcc
    13441 tgttttttca gagttaatta tcgccgttga ctcgggaatt aaatgtaaaa aagcatctcc
    13501 gaggagagct ccagtggcta ttcccactag ggtcaacagt tctcccttca atctattttt
    13561 tttaatttta ataaacagta ttccgagaag agaaaccaga cttattaatc cagaagcaag
    13621 caaagccaat aatattggat tcatgggcac cattctactt catttttttc ctttctgcca
    13681 gttataatag tattgtgcaa ccagaagatt ttaaatcacc tctcccacca cggcggatta
    13741 ctgttgtcga tgagccggtc gaggcctctc ctccaccagc ctccgtcaat ccccaggtaa
    13801 cgccagaaat tgttcccacc cgtggcagtc ttactcctcc taaaaaatca tttataccct
    13861 atattatagc tatttttgct attgccttac tcgctttctt gattttcaaa tttatcctcc
    13921 cttatttcaa taaagatagt tctaaaccag tgacgattac ttactgggga ctctgggaag
    13981 attccagcgt ccttcaaggg ataatttctg attttgagag taaaaatccc aatatcaaaa
    14041 tcaactataa aaaaaatgat aaaaccgatt atcgaactcg tctggctgga cgactgctca
    14101 aagatccaga gagcgaggag gttcctgata tttttagaat tcattcttct tggctcccca
    14161 tgtttaaaga tgatatttca ccagtcccgc ccgccactct taatgctata aattttgaag
    14221 aagattttta tgatgtttat aaaaaagacc taaaaatagg taattcatat tatgctatcc
    14281 ctttaatgta cgatggtctt gctctttttt acaacaagga tttaattcaa aagggagggg
    14341 ttgagttacc caaagactgg tggagtttaa aaactgccgc ttcaaaattg actgttagag
    14401 atcaaaatgg caatattgaa attgccgggg tggctttggg tctcaccgac aatgttgatc
    14461 actggagtga tattcttggt cttttattaa aacaaaatgg ggccaatatt ttaaaattag
    14521 ataaaagcgc tgaagataac aagcaaatcc aagatgttat ttcttattat ataaattttt
    14581 acagcgagga aaatcgtccc tgggatgaaa cccagcccag ctctactgaa ctttttgcta
    14641 gtggtaaact cgcgttttat attgctccct cttggcggat ttttaatctt gatgaaataa
    14701 acccaaatct gccttttgaa attacttcta ttcctcaatt gccaatacaa caaacaggca
    14761 atttaaccga tattcactgg gctacctatt gggttgaggc ggtaaattca aaaagtaaac
    14821 atcaggccga agcctggaaa tttttagaat ttttggcctc caaagaatct cttcaaaaaa
    14881 tgtataccgc ggcttcagac acccgtcgtt ttggagaaat ttatcctcga aaaagtttgg
    14941 gagagagctt gaaaaatgaa ccaaaactaa aaccttttat tgacagtgct aattccagtt
    15001 atgccgaatc cggttattta gcctctcgca cttttgacgg cggtggcata aatagtgatt
    15061 ttattaaata ttttggtgat gccattaatg ccatcgttaa aaataacagt gacccagagg
    15121 cggtcatgac cacgttaaac aatggtctta atcaaataat tcaaaaatat aaactcaatg
    15181 atattgctca actttaaatt gtatcctgaa acctttggtg atgaggccat aaatttatct
    15241 gccattgcca agcaggttat ggaaaatact ggggtaaaaa taattcctgt cctatcggct
    15301 cttgatgccg tcagagtcaa agaaaaacta aatattgatg tttatcttca gcatgttgat
    15361 gtctactcgc agggtcccca cactggatat gtttctgccc tagccgctaa atcattaggg
    15421 ataaaaggcg ctttgataaa tcattctgag catcgtttaa aattggggac tcttaaacaa
    15481 attttaaaat cttggccagc cgattttgat tcagttttat gtctccaaag ctaccatcag
    15541 cttttacgct gggctttaaa actaagggtt aaatattatg cctatgaacc aaaagaattt
    15601 attggtaatc ccaatctttc cgttgcctct gctcggcccg aacaaataag caattttgtt
    15661 aaaaccttaa gtccaaaacc tattttagtt ggtgctggaa ttcattccag ggaagatgtc
    15721 tcaactagtc taaagctagg agcttcagga attttagttt cttcttcagt tatcaaatca
    15781 gctgatccca aaaaagaatt aatggccctc gcttctgttt ttagtgtata attaacaagt
    15841 ttatggcatc aatcgataat cttttagttt ctctcttcgt tgacgaagtt gtcggcggtt
    15901 ttttagttac cgttcctcct ggtcacgttg gttgtatcta tagtattttt cgtggggttt
    15961 taaaaaatgt ttggtacccg gggatgcatt ttaagatccc aatcattcat cgggtaaagt
    16021 tatttaacgc ccagcttatc gaatacacta tttccaaaga catttatctt aaagacaata
    16081 aagagattat gggtgacgaa ctaattcacg ccgttaccgc ggacaataaa tttgtcgccg
    16141 ttgaagctac cgttttaatc aaattggata ctgatagatt gccggaaata tggcaaaata
    16201 tcggtgaaaa ttttgtttct aaggttgttc gtcccgttac tcgtagtcgt atccgcaaag
    16261 tccttactaa tcacactcta gtcaagtcat tatcgactga ccgtagttta atcgaagacg
    16321 aaataaagca agagcttcga gattctctaa cccagcatgg tctctacgtt gaaaattttt
    16381 atttatcttc tctcaccccc attgatgctc cctttaatat agaagagacg actgaattac
    16441 cggttcttat ttctggactc cagccagagt caagccccag cccaaattca cccccgactt
    16501 cttcaaggcc ttcagtgccc cagtcatagt cgccccgctc gtaatgacat cgtcaaccaa
    16561 aataattttt ttaggaatta ttttattgtc taccacctga aaaatatcag ccatattttt
    16621 ccacttttcc tctctatttt taattttagc ctggcttttg atctttattt ttctcctgat
    16681 taaattattt ttccatccta attttaattc tttcgatagc ttttccgcca gtatctcact
    16741 ttggttaaaa cctcgccatc tttgtctctg agggtggaga ggaatgggaa ctaggatata
    16801 ttttttttct tgccagtatt taacgatatt ggggtaatct atttttaatt ttctcgccat
    16861 cttcttagcc atttcctcaa tggcgtcact aacaaatcca tattttattt tttcaataat
    16921 atcttttatt aatccatcat atttaaagat tgaaataatt ccctcaaatc catctttttt
    16981 tattaattcc ccgtttttta ttttagtctc acataaagtg cagaggtact cctctccctg
    17041 accacagcca aaacactttc tcggaaataa ccaattgatt attaaagaca tcttttaatt
    17101 gtaagataca ttgtagattt aataaaaggg acagatgcct caaccagtct tttagatttt
    17161 catctgggct attcttagtc tctttggggt aaatctacac tcggagtcgc ctcaattttt
    17221 agtggtcttg gttggggact cttcattgac gagatgtcaa attat
//
LOCUS       DHGB01000049            9158 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_29482, whole genome shotgun sequence.
ACCESSION   DHGB01000049 DHGB01000000
VERSION     DHGB01000049.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_29482"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    234..329
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1449..1477
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2795 a         2017 c         1528 g         2691 t
ORIGIN      
        1 gagatgctta cttcaaagcc tttatctaaa aaaatatcaa taaaagaagc aaaaacaggc
       61 gatttaattc ggcaaaattc cggccatcat ctcttgttta ttgtcgaaaa aatagacaat
      121 cagataatct acgttgattc ctcacttaag ggcaggggag ttcgttatgg gcagttcgat
      181 atcacggaca aaacctttaa atatgacgga atttttagat taaatcgata aatnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgcaatcagg acaaataact ttattattag
      361 tatctggcat ataatttagc taaaatataa tcaattcgat tattacacat ctattaatct
      421 aatcaactat taatctatac atctaataat gacaccttta atcaaaatcg acaatctcta
      481 tttcaaacgt gaagatctaa atccgaccgg ctcagccaaa gatcgggcca ttccttttca
      541 aattcaaaac ctaataaaaa ataactacaa ttccgccgtt atttcttcgt caggcaacgc
      601 ggccatttcc gcctcatttt attgtcagca ggcaagtatt cccctgacta tttttgtctc
      661 ccccaaaatt aatcctcata aattagccct tattaagggt catattatcc aatcgatcca
      721 accaatcagc gatgcgatta aattttccaa agcgaatcat gcctattttt tacgccaatc
      781 aactgacccc agtgcccaaa gaggctacgg tgaaattgcc aaagaattac ttgagcagtt
      841 accccaaatt agcagtctgt ttatacctgt tggcagcggt accaccttcc ttggaatcag
      901 taaaatcctt ccccataatg ttaaaatctt cgcagttcaa ccggccagca atcccaccat
      961 ctgtagtatt tttaatccag tttatacgcc agaaaatgag accattaccg aagctcttag
     1021 tgtcaaatac ctgcctctca aaaaagaaat tattaaaagt atcaaaagcc atggcggcag
     1081 tggtctgatt gtcaccaatg aggaggcaat taattcactc gaatttatca atcagtttaa
     1141 tgtctcgcca gaatctgctc tcactctcgc cggctatctc caagccaaaa ataattttaa
     1201 aataggagaa tttccagtta ttttattaac cgggacaaaa agatgaccag caatttagaa
     1261 aatcttttaa ataattcagg ttttaatttc ttctttttgg ttgtcgataa atttttagat
     1321 atcaatttac cccagttaaa aaattttaag tccctctccc aaaataattt aaaaatcaaa
     1381 aatagtggtt tccttttatc tcaaaaaaac atccaggctc aaattaaaaa gagtcaaaat
     1441 ccggtcatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt ctcgaggata aaattaagtt
     1501 ttaccagctt tgccaaaaaa acaatctacc ccaaattccc gcttttatcg ctccatttac
     1561 tcaaaataat ttttcaaaag cccaaaagct ctttggtcaa aaaatcgtta cccaaactca
     1621 ttttggttgg gctggcaaca gtacccatct ggccttaaat tactctgaca ttaatcacca
     1681 aattgccgac caaaccatga ccaaattttc cccttttaaa aatggatatt cactcctaaa
     1741 caattgctgt ctcacccgcc atggcctcct ccaaagtccg cccgccctcc agtatactgg
     1801 tctcaaatct ctcaccagaa atccctttgc taccgtcggc cgacaatggc cctctcatgc
     1861 tcctactgaa attattaata aaattaatca aataacagct gaattttcta aaaaaattct
     1921 cgagccctta aattatcaag gtttttttgg cctcgatttt ttaatttcgg ataatgaaat
     1981 ttatctctta gaatgtaatc ctcgcctcac tgcctccttt gctttttata cccagatgga
     2041 gttaaccaat aatttaaccc ctctgtttta ttttcatctg gccgaattta tagatttaaa
     2101 ctatcaaata gacctaagca cagagcaaaa aagattttat aatcaaaatt taattggctc
     2161 agaaatcact cttagaaacg ggcgggggac aactattaaa aaattaactg attttaaaat
     2221 tttttcaaaa aatattaatc ctccgattat tgatccccgt attatcagaa aattaaaaac
     2281 atgagagtct cccccctgct cacctacgcc tctactcttt ccttggttgg tcttctcttt
     2341 atttcctctt cttctctctt tgaagctgaa aataccattg gtgatcccta tttttttcta
     2401 aaaaagcaac ttctttggct cctgattgga tttgtggctt tatttattac ctcaaaaatt
     2461 gatctgacca ccgtcgtcaa taattctttt cgtttttatc tattatcgat aattttactg
     2521 attttggtcc taattcccgg aattggtttt aagtcgctcg gagctaggcg tcagattgat
     2581 ctgggcttta tcaattttca accctctgaa ctttttaaac tggcgtcaat catttttttt
     2641 ccttatttat tttccttaac cgaaaaaaga aacttgggaa atttagctct ctacctggga
     2701 attcccttta ttttaattat attggaacca aatttaagta ccgccgccat gattgccgct
     2761 attgtcatca ccatttatta tttatctgga gctgaaatta tcagtctttt ttttgccgga
     2821 tttacggttg taattttaag cttagcctta attgctattt cgccttatcg tctcaaccgg
     2881 gtcaaaaccc ttctcaatca gcagcaaaat gaagacctca gttatcacag cgagcaaatg
     2941 attctttctc tcgcctccgg ctcctggtcc ggaaagggtt ttgccaactc agaccacaaa
     3001 tataaatttc tccccaaaat tagcaccgat tccatcctgg ctataattgg tgaagaaata
     3061 ggttttgttg gtttaactct aattacatat ctttttttac tcctaatctc ccgcctaatt
     3121 cgtatttccc aggttgcctt agacccttgg gaaaagctgg ttgtcggcgg gattgcctgc
     3181 tggattgcct atcaaggact aattaatttt gccgctattg tcgccataat tcccctaacc
     3241 gggattccct ttccctttat tgcctatggc ggttcttctc ttatcagcct ctttgctgcc
     3301 ttaggcctgg ctataaacat cgaaaataaa tacgcaaaat tgttatactt taaaaatgac
     3361 cgaaacgaga aagataatcc tcaccggtac ccatctcact cccgccattg aatttatcaa
     3421 ccaattaaaa acagacccag aaattgattg ggaaatttat tatcttggcc gtcttttcaa
     3481 ttccagtatc tcaagagagc cttcaattga atccactatt attcccaaaa tagggataaa
     3541 attttacggt atcaactgcg gtaaattcga ccgtcgctgg ctaccaaaca ccatcaaagg
     3601 cctccctcaa atatatttgg gttttaagca ggctcatcgc cttatcaaaa aaataaaacc
     3661 ccacctggtt gtctcttttg gcggttatgt ttccgttccc gtcatcatcg ccgcagctac
     3721 tcaaaagatc gtgtccttaa ctcatgagca aactcaaacc ctcagtctgg ccaccaaaat
     3781 aaatgccctc ttttgtcgct atgtggccct ttcttttcct ctcaaaacta attcagacaa
     3841 atatctagtt accggcaacc tcctgagacg agaaatattt aattctcatt caaaaaaatt
     3901 tgaaagagaa aaatttaatt taaaaaaatt tcctttatta ttttttactg ccggcaatca
     3961 aggttcacac catctcaatc ttattttaaa aaatttgcta ccagatctat ccctaaaata
     4021 caccataatt catcaatgtg gtcaaaaaga ttatcccttt tttaaaaaac ttgcctcgaa
     4081 ataccccaat tatctcgcca ttaactatat cgatgggagc gatattggct gggtttttca
     4141 tcactgccgc gttattatca gccgtgctgg ggccaatacc attcaggaga tagaggcttt
     4201 aaaattattt tcgattatta ttcctctacc aattagtcag caggacgagc agttaaaaaa
     4261 tgctctttgg ctccaaaaaa aatctcctca atctactatc attataaaag atgccgatat
     4321 taccgaagaa aaattaacca aggctattaa aattttagtt tcaaaaaaaa gaaaatcgac
     4381 tatcaaatca gcttctccta atcttaggct attaaaactg atcaaaaggc tctaaaatta
     4441 atctatgcaa cattttaaaa taattaattg tcttgacatt ggcacctcaa aaataacaac
     4501 tatcaccgga caatttcccg aaaacgaaag caagataaat attgtcggcg tcgcctctat
     4561 tcccgctgtt ggttttaaaa aaggccaaat tataaactta gagcaagcct caaaaaccat
     4621 cactgattgt atcgaatcga ccgaaagaat ggctggtttt caaatcaacc atgcctatat
     4681 ctgtctgact gccccccacc ttgaatcgat aaattccaaa ggggtaacgg ctgtctcagg
     4741 gacaaatggt gaaattacca ccgctgatat tgagcgggct gttgaagctg ccaaagctat
     4801 ctccctgccg gcgaacaagg aaataatcca tgtcattccc cgtctcttta cagttgatgg
     4861 tcaagagggg gtcattgatc ccgtgggaat gagtgggatc cgcctcgaag tcgaaactca
     4921 tttaatttta gcctcaactc cttctctaaa aaatctctat aaatgtcttg aagatattgg
     4981 cataaaagtc gacggcctcg tctattcagg tcttagcgcc gctaaagctt gtttgaccga
     5041 aacagaaaca gagctcggtg ttgccctcat cgatattggt ggaagtaaca ctactttgac
     5101 tatttttcag gaatcatcgc caattttttc cggcgttatc cccatcggtg ccaataatat
     5161 tacgaacgac ctcgccattg gcctccgcct cccacttcaa gattcagaaa aaataaaaat
     5221 taaactcaaa gcctatgccg ataaaaaagg ttttgaagat gaaattgaat taactcatct
     5281 ggggattatc ggagaagata agaaaaaaat ttctttttcg acaacagtta atggcattat
     5341 caaagcccgg cttgaagaaa tttttggatt tatttacgat caaatagaag ataatgattt
     5401 taaaaattct atcccagcag gtattgtcct cacgggtggg ggagcccaaa ctatattagc
     5461 caaagaagtc tgtagtgata ttattccctt acctgtcaga atcgctgagc caccgcgtct
     5521 cggaggcctg gttgatgaca tcactaatcc agctttctcg agtaccatcg gtactatttt
     5581 atattttcaa aataataaaa gccccgtcaa atcaaaaggg gccaataaaa aaacaaaaat
     5641 ttcttttgaa ggaatttttg gtcgactaaa aagttttatt gaaccacttt taccataaat
     5701 atgggactta tcaaaacgct tactggtcaa tttgccaaaa tcaaggtagt gggcattggt
     5761 ggtgggggca ataatgccgt taattcaatg atttctgaag aagaaataaa gggagttgat
     5821 tttgtcgcca ttaatactga ttctcaggca ttattaaact ctctcgctcc cataaaaatc
     5881 caaatcggag aaaaaattac caaaggcctc ggtgctggag gcgatccttc cattggtcaa
     5941 gaggctgcgc aggaatcaaa agagcgtatc aaagaagtcc tcgaagggac cgatatgctt
     6001 tttatcactg gaggtatggg tggaggtacc tgtactgggg ctgctcccat cattgctgaa
     6061 attgccaaaa aagaactcgg aatcttaact atcgcggtcg ttaccaaacc ttttttattt
     6121 gaaggtaccc ggcgcatgag caaggccgac gagggactct cgcgtttacg ccagcaggta
     6181 gatactctaa ttgttattcc caatcaacga gtcatggaag tcgtaaattc gcaggcaact
     6241 cttcttgaag cctttaaaat tgctgattct gtcctaacca ggggcaccaa agccatagct
     6301 gatttaatta cagtccctgg acttataaat ttagattttg ccgatattaa atcaattatg
     6361 aataacgccg gctcagctct gatgggagtc ggtgaggccg agggagccaa taaagctcaa
     6421 gatgctgttg aggccgctat agattcaccc ctagtcgagg ttaatattga tggtgcccgg
     6481 ggagttctta ttaacatcac aggtggacct gatctaacca tgtcagaaat tgagcagtcc
     6541 gccaaaacta tcaccgccca tgttgctcct gatgccaatg tcatttttgg agcttcaata
     6601 gatcccgatc ttaccgataa aataaaagta accgtcattg ctaccggctt tgattcgagc
     6661 aaatcttcaa cctactcctc ttacaaaaaa cctttatcac tccaaaagcc ccttctcgaa
     6721 gttgatccag atactcaaaa agaaattgac cccaaacttc aaaatctcct ggctgaccaa
     6781 gaagtgccag aaggtatcga catcgaagat gaattcgaca ttcccgcctt tttgagaaaa
     6841 aataattaag gttatagtat aatttcctat attatggcct actttaaaga gctaccaaac
     6901 actcctaatt ttcctctcat tgaaaaagaa atattaactt ggtggcagga aaacgatatt
     6961 ttaaataaat atctccaaaa aaataattcc tctgaaaaag ttttttcttt tattgatggt
     7021 ccaattaccg ccaataatcc catgggagtt caccatgccc gggggagaac cctcaaagat
     7081 gtttttcaaa gatttaagag cgctcagggg tttgcccaga ggtttcaaaa cggttttgat
     7141 tgccaaggac tttgggtcga ggtcgaagtg gaaaaagaag ctggctttaa ttccaaaaaa
     7201 gacattgtaa attttggggt tgaaaatttt accaaagcct gcctggctcg tgtcgataag
     7261 ttttctaaaa tccaaaccga gcaatcaaaa cgtctgggga tgtttatgga ctgggacaat
     7321 tcttattaca ccaattccca aaccaataat ttatatatct ggcatttttt gaaaattatt
     7381 aatgaaaaag gctggctcat taaaaagaaa agtgccacca cctggtgtcc tcgctgtgaa
     7441 accggtcttt ctcaacacga acaaagcgat gcctatcaaa tggacactga tacctcagtc
     7501 tatctaaagt ttaagttgaa agataggcaa aatgaatata tactcgcctg gaccactact
     7561 ccgtggacac tctcagccaa cattcttctc gctatcaatc ctgattatca gtacgttaaa
     7621 gccaaagtag acggtcaaat tttatatctt gccaagatag cggctgacag actggccata
     7681 actgattacg aaaacattga ggcttcttct ctcctgaatt tagaatatga ttctctttac
     7741 aatatacccg cccaaaaaaa tatcaaacat tatgtcaccg cctggaacaa ggtaaatccc
     7801 gaagagggaa ctggtgttgt tcacctcgct cccggctgcg gccaagagga ttttgagctt
     7861 ggcagccaac tcaatagcga tatgctcgct cctcttgacc cgagtggtca ctttttggag
     7921 ggatacggta atttaaccgg actttacgcc cataaagtag ataacctcgt tattgaatat
     7981 ttaaaaagta aaaacctact ttataaaacc gaagctatta cccaccgtta ccctcactgc
     8041 tggcgctgca aaactaaatg tctttttcgc cttgaagatt cgtggtttat caaatcagat
     8101 gaaattagac ctctcttaaa agaagccgcc caaaaagttt cctggcatcc aaaatatacc
     8161 ggtcgccgaa tgcaaaactg gctcgattcc atggatgatt ggatgattaa tcgtaaacgt
     8221 ttttatggac tcgctctccc tttctatgaa tgtagctgcg gagaactgac agtcattggc
     8281 tccaaaaaag aattaatcga aaaagccatt catcccgaac tggtcgacaa actaccctct
     8341 tttcatcgtc cctggattga tgaaatcgaa ataaaatgcc ctaaatgcgg acaaagtgtc
     8401 aagcgtattc cggatgtcgg cgatgtttgg cttgatgccg gagttgttcc tttttccact
     8461 cttaaatatc ttgaagataa aacttactgg caaaaatggt ttccggcaca gatggttctg
     8521 gaaatgaccg aacaaattcg cctctggttt tattccatgc tttnncttta gtgtcgtttt
     8581 agaaggagaa gccccttata aaaccgttgt tactcatgcc gaagttcggg acgaaaaagg
     8641 ggagcgaatg agtaaaacca aaaaaaatgg tattcctttt aacgatgcta tcgaaaaaat
     8701 gggcgctgac cccatgcgct ggctttattg tcaacaaaaa gcctatacca gtgtcaattt
     8761 tggttacaac atcgccgatc aggtcaaacg tgattttctc ctcattctct ggaactctta
     8821 tcgatttttt acccagcatg ccaatttaga atcttggcag ccaacaccag acgtcattgc
     8881 gagtgaagcg aagcaatcat cagagcctat agataaatgg ctgttttccc gcctaaatca
     8941 cactatcgaa atagtcacca ctaatctcga aaaatataat accgccaaga gcaccgctgc
     9001 catcgaaaaa ctcgtcagtg atctttctac ctggtatatc cggcggagtc gggaaagaac
     9061 agataatttt tctcttcttt tctatgtttt tgataatatt tttcgactta tggccccaat
     9121 tacccccttc ctcagtgagg ttttatacca gaacctac
//
LOCUS       DHGB01000050            5732 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_123479, whole genome shotgun sequence.
ACCESSION   DHGB01000050 DHGB01000000
VERSION     DHGB01000050.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_123479"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1681 a         1012 c         1098 g         1941 t
ORIGIN      
        1 aaatcaacta aaatccatga tcgaacaggg ttacttcctt ttaaaatagt ggtttttgat
       61 ttcatttttt agtctccttt ttttggctag gggctgcttt atttttagca ggaatagttt
      121 ttgcttttgt aatttttttg ttatcttttt taatttcttt tttgactttt tctttaataa
      181 cggggagact gtttacttct ttgataaaac ttagtctaac aattaaagca ttgtctcctt
      241 gacggtattt gacgtttttt aattgagtaa agttttgagt ctggccagga aaggtagtgg
      301 aaaaagcggt tacgattctg ttgacgacat ttcgatcttg aaaaaaggta aaaatatagc
      361 gacgagaatt aagatcagct ttaacggcag agagggcaat tttttcagcc aaaggagcga
      421 tagattttgc cttggtgaca gtggttttaa tataaccatg agtgaataaa gagcgaagct
      481 gagacctgta aagatttttt ctttgattgg tagttcgacc taaatggtaa cctgaaattc
      541 gatgtctcat ctttatttat tccaggaaaa tcccttttcc tcaagccaag catcaataat
      601 ttttaatgat ttttcaccaa cgtttttggc tttactaaca tcggctcggc ctgctttaac
      661 taaatcatta atagttgaat aatcagcttt tttgagagcg ttagtaacgc gaagaggtaa
      721 ttcaatttcc tcaactgaaa tctcagccat ttgattgatc ggcatggaat ctagggtgat
      781 gacttcatct tcaaattctc tggtatcaac aatctgatta agagcgttaa ttaaatcttt
      841 ggcggccatt tttaaagcgg ttaaaggctc aattgaagca tcagtccaaa tttccatagt
      901 tagtttgtcg taattacttt ttttaccaag acgggtaggt tcgacagtat aattggcctt
      961 taaaactgga gtaaaggtag catcgagaat gacttcaccg atgatatcag ttttttgttc
     1021 acgggcgaga gtataaccaa ccccacgctc aacagtcatt tctattttaa ggtgagcatt
     1081 ggtatcagtt aaagtagcga taacctgatc aggattagaa actttacaga cagcactatc
     1141 agaaatatca ccagccgtaa cgaccccctt acctttttta tctaagatta aaactgtagg
     1201 ttcttccttg gcataggcaa atttaatttt tttaagattt agagaaatct caacaagatc
     1261 ttcttttact cctggtaagg tggtaaataa atgggtagct ccatcaattt taatgtgagt
     1321 gatggccgcc ccaggaatag tgattaaaag aattcgacga agactattgc cgagagtatg
     1381 gccaagacct ccaacgagtg gggtcagttc aaacttgccg taggtggaag tttgtttaat
     1441 agttttaata tgaaagtgac tggtgtcgat catagttttc tcctttgatt atcttgaata
     1501 atattcaata attaaattta catcgatccc gttatgattt tgatcgatga tgggaagtgc
     1561 aataattttg gcactggatt tggatttgtc taaatcaatc cactccggag ccttaaagtc
     1621 cttgtcggcg tatttaaaag tatcgccatt tttttcaagg gttttgggtt ttaaggtaat
     1681 taaatcgcca actttaacca aataagaaga aatgtttaaa accttaccat tgactaaaac
     1741 ttctttgtgg ctaatcaact gcttcgcgtg gcttctggat aaacaaagtc cagaaagata
     1801 aacaacatta tcgagtcttc tctcgacaag gattaataaa ttttctccga ctaaaccttt
     1861 taatttttta gcagcagtgt aataattttt gaactgagtt tcagcaacac cgtaaaaacg
     1921 tttagctttt tgtttggccc gaagctgaat cccgtaatcg gtgggcttcc ggcttctttt
     1981 ggtaccgtgc atgcctggtt taactgcccc tttcttttct aggggacatt tgggggaaaa
     2041 gcaacgagcg ccttttaaat aaagttttac gccttcgcta cgacagagtc gacatttagt
     2101 gtctagtgtg cttctcatgg tattttttta aactcggcgg gccttgggag gtctggggcc
     2161 gttgtgagga actggggtaa catcggcaat aagatcgact tcaaaatcgc gcttggcgcg
     2221 aagaaagcga agagcggcat ctcgaccggt acctggtcct tttaagtaaa ctgctaattt
     2281 tttgattcca atgggtctga cagcttcaag agctttttcg atagcagtgg tggcggcata
     2341 aggagtagat ttacgtgaac ccttaaaacc acactgcccg gcacttgacc aggcaagagt
     2401 atttccctta atatcggtaa tagtgacaat tgtattgttg aaagttgact gaacgtaaag
     2461 tcgaccttca gccaaactct tgaccatttt tttggtcttg gtaagagatt tggttttttt
     2521 tatgctttcg ttagtggtag tttttgcttt gcttgttgcc atatgattaa aattttattt
     2581 tttggcgacc tcggcagctt tttgctgggt ttcaattttt gcccaagctt ccttggtaag
     2641 agaaccaact gttttctttc tccccttacg ggtacgagaa ttggatttgg ttctttgacc
     2701 acggacaggg agagagacaa tgtgccggat tccacgataa catttaatcg cttttaaacg
     2761 atcaatattt tcacgaattt ctctttttaa atcgccttct gtttgaaaat cttcgagagc
     2821 tttcatcaaa gaaccaatat catcacggct taaagttttt attctggtat ttaaatcaat
     2881 accggctttt tgggctagtt taataacatt tttaggacca ataccataaa atcgagttag
     2941 agccacctcg gctctctcat tatcagggat ttcaactccg gaaattctca taaaaattaa
     3001 ccctgttttt gggtgtgtct cttggtagaa caaataactc gctttacacc ccgtctgatg
     3061 acgattttac agtttttgca gcggggtttt atgcttgatt ttactttcat aaactttggc
     3121 tttggtattg taacctaaac tgccgataat atcaaagagt aaatttaata acgaggactc
     3181 aaccgcggta ggttatccga cccttgttgc catcgggtga taaaacaacc gaaacagtgt
     3241 cacccgccag acaacgaatg tggtgcattt tcagtttgcc ggctaaataa cagacgataa
     3301 tacggtcgtc ctcgagcttg acccgaaata actgatcggg taaaacttca gtaacagtac
     3361 ccttgacttg tggttctttc atttagattt aattaataat atttattttc tgagtgattc
     3421 gacaatttgt tttaatttgt cgaaagtaaa agataattct gcttctgttg tatatttact
     3481 taaactaaag cggatagtgt ccattttagc agaagtaccg cgaattgctc tagcaacatg
     3541 actcggttca agagataaag aattacacgc agatgaaacg gaagctgaaa taccatatga
     3601 atctagcatc aaaacaagac ttggagcatt tattccttca aagcttatgt tgatgttgtt
     3661 gcataatcgt aaagtaggat gaccgttcag ggaagtgttt ggaatttcgg ataaaatttg
     3721 cttgatgaaa ttatccctaa gattagtcat attaagaata tcagatttag tatgtagatt
     3781 gacagccgta gcaaaaccca taattccagg aatattctcg gttcctgaac gaaaaccttt
     3841 ttcttgacca ccaccatgta gaagtggggt aagattaatg ttttctttta cataaagggc
     3901 ggcgacgcct tttggaccat gaattttatg agcattgatg gtaaccatat caacataatc
     3961 ctcttctatg tttaagggga atttagtaaa actttgcgag gcgtcggtat gaaataaaac
     4021 tttattttcg cgacagattt tgccaatttc agcaataggt tcaatagtac ctatttcatt
     4081 attagcctgc attactgaaa ccaaaacagt ctctggcctt aataaatttt ttacatcttg
     4141 gggattaata agaccctgtt tgtctacagg aagataagtg acatctacat ttattcctag
     4201 ctgagcaagc cacttggaag cattcaaaac acaatcatgt tcgatttcac tgataattag
     4261 atgtcttttt tgttcaagcg ggagtgaaag aactattcct ttgatagcta aattattagc
     4321 ttccgtaccg caaccggtaa aaataatatc accagtcttg gcacctaatt tagcttttag
     4381 tatatttttt gcctcaatta tggcattttt tgacttttgg ccgagtgtat gaagggaacc
     4441 ggcgttaccg tataattctt ccgaatattt tttaacaact tcaaaaacct ctggtgcgac
     4501 tttagtggta gcggcgttgt cgagatatat ctgattattc attaatgggt ggattaacga
     4561 tttgttttat tttagcaatc aattctggtt ttttatctgg agtaaaatga ccctcaacac
     4621 aacccaagta tttaccttta ttaaaaatca tcagacaagg taactgggaa attttatatt
     4681 ctgaaaggag agcatcatcg acaatattaa ttttgtgata attgtaattg ttattttcaa
     4741 aaagttcttt gataggaatt gaattaatca ggcattctgc agtttcgttt ttaaataatt
     4801 caataaaggt taagacattt gattcttcgg cgcaggtttt aaattgtgat tgatcataca
     4861 aaaaagcttt gccgacggga gcagctccgt aacgatcaac aaataaatca gcaatctttc
     4921 gaggatcatt atcaatttca agtttgattt tttcatattc tgattgatcg tgagcgatat
     4981 gaatagcacc aaccggacaa ttttcttcgc aaagaccaca actaatgcat ttttcattat
     5041 caattgttat tgttttttta tcgtcatccc aaaatattgc ttttgtagga caagctgcaa
     5101 taccaccaca ttctttagcg ttatcacaga tttttctatt gattaatatt gacattatat
     5161 tgctaattta ttaattttat tacaaatatc ggtaaaaata ggttcgatgg ggcgattacc
     5221 gtcgatatca acaaaaagac ctttggattc aaagtattcc cgaatgggag tgataccatc
     5281 atcataaaat tgctgacgtg aagtgatagc ctccggagta ttgtcagttc tattcgagtc
     5341 ttgaaattcc tgacctaatt tattccgtct agcggtgatt tcctcaaatg tgacactgag
     5401 atgaattaat aaagaaaagg gtaaatcttt tttctttaaa taatattcca tatatttgcc
     5461 ttggtttaac actcttggga aaccatcaat aataaaattt tgattgttaa tggaatttaa
     5521 tttggcgact aaaatatcaa aaattaaatc atcgggaact aattttccat tatcaagata
     5581 tgatttggct tcgtcgccaa aaccattacc agcggcaatt tcatcacgga gaagctgacc
     5641 catggataag tgagtcaaat gatatttttc cgcgataagt ttagcttgag tggatttgcc
     5701 gcagccgctc gggccgatga taaaaagatt ta
//
LOCUS       DHGB01000051           17663 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_62364, whole genome shotgun sequence.
ACCESSION   DHGB01000051 DHGB01000000
VERSION     DHGB01000051.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 17663)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17663)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17663
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_62364"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    7756..7782
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8827..8850
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6160 a         2404 c         4267 g         4781 t
ORIGIN      
        1 aaggcaaaag cagaatttgt taaaaacata aaccaaaatc taccccctac ccctcctttg
       61 actaaaggag gggagaatag aattaatgaa aataaactta atgaggcgaa gcccgctgcc
      121 gttgataact ccagcgagac gaagatgact tacgataagt tgtttggaaa cgatagtacg
      181 acttaaggta taatttttaa atgagccaat tgccaggaaa tattacggaa ttaattgcgg
      241 cacttgaagg gctaggtaaa agaaatttaa atgaagagta tgaacttttt gaaacctgca
      301 gaggagtatt tgaaaagcta agaagactga gtgattttga cccgagtgat ttaaagaaaa
      361 cgatcaaggt gttaaaggat ttgcctttgg aaataaagga attagaaatc tataaactcg
      421 atattgagac cgagaaatta gaaagcaagg ctcttaataa acaggactta aaagaattgc
      481 tcgaaaacta tgaaaaagct acaaatgaaa acgaaagaaa aagaatagaa aaagaaattt
      541 acgcaaggac tggtcaaaaa aatgttgaac agtttattaa aacccaaaaa gaaattgcaa
      601 acaaaaatgc cgaaaaactc agtaaacttg atgaagaaat aaaggaaggg gtagtagaaa
      661 atttaacaaa aattaaacta gaagagaagg aagaattaat cgatattgtt gaagaagcaa
      721 gtttggagga gaaatttgat gaaaaaaaat taaaaagaga aataaaaaaa agaataaaga
      781 atccggaaag ggttgaggaa tttattcaaa aagtcgaaga aattagagct gaaataaaaa
      841 ttaaagaaag ggcggaagaa atcgctaaaa ctacttatga aaaattagct gaagaaaagt
      901 tgccaattaa tggcgatatt aaagaaaaaa ttaagaatga aatattaatt gcctggaaag
      961 aaggagatag aataaaaata ccgaaggaac ttgaagaaat aagtgaagct aaagtaatta
     1021 ttaaagaaac agaagaggcc gctgatattt ttaaaaataa aaatattgaa gcaattgtaa
     1081 attaccgaag cctagaactc ggaaagaaaa caagtcaaga acttagaaaa aatggagttc
     1141 aggacgagtc tttaattaga gaatatgtct cggtagtaaa ggagctgact aacaatcctg
     1201 acaacgccag gatcgaggaa aacaggagtg atatctataa ttttgttcaa agcgagaatc
     1261 caaataaggg cccaggagag atggaaaggt caattgatga ggccagattt atagcaaata
     1321 atgttgtaac ctcaccaaaa aagtttaata aactgattca aaaatataat ctactgagag
     1381 aaaaaattgg cagcgataag ctaccaaaac taaaagaggt cagagtcctg gaaaaaatga
     1441 cgactctttt tgtaaacaac ccaaaaatgc tgaaattgtt aaatggaact caaaaagtag
     1501 caaatattta caataaagtt gttacttttc ctagcagaat gcttggaaag ttgactgttg
     1561 gagtcgtaga aaaaatagaa ggaaaattag caaaaatagg agcacaggca gcgacagaat
     1621 ttgttaaaaa ttcagccgcc ataatcgcca aagaggggac ggcaaaaggg ctgggaataa
     1681 ttttaaaatc cattttggga aaagggacag ttatagcagg agaaggagcg gcaagtggtg
     1741 gagcagttgc tggtttagta actgcctttc aggcactgcc ggtagttgga caaataatag
     1801 ccgtagtggt agttgccgca gcagccgtat tggcagtcgt aaaaccgata atcgatggag
     1861 caaaaaaaat tctagaaaaa gtcttgggaa caaatcttaa taaaattaaa aaatttgtgg
     1921 ccgaagattt gggactgggg aaatttattg gaggggtgac tcaattcctc tttgatttgt
     1981 ctgttttttt tgttggaatt ccaaaactat ttggattaat taaatttagt gcgactttga
     2041 cgccaatatt tattgctttt tttgcgggaa cttttttata caacatgcta atgcaccaac
     2101 aaatatcttc aattgttcca cctgcggatt tgagtggttg tattttaaaa agaggcagcg
     2161 gtccgggagg attacctgaa ggagagatta actgcgatat aaacgcacca gaaaatgaag
     2221 taccgggttt aattagtaaa gaaaaatatt ttgatgttgt taaccgatgg agttcgggta
     2281 aaaagtatct tgatgagtgt tataacgacg tagtcaacag atctctttgt gccgggatta
     2341 atcctcttta ttctctgtgg gtttggatgc acgaatcggg tgcaagcaat tatagtcttg
     2401 ggaggattca ggattttgga attaatgata gttcaattga aaataatttt gatgctcaaa
     2461 ttaaggtttt tttaaaccta gatccggcca gtgcgtgcga tttaaatgat ccgaaattaa
     2521 gaggccccga tggatactgg ctcgcttggg cttctagata tttgacagga caatgtgatc
     2581 ctgatgtagg acaggcacaa actggaaaca cgggcagaaa atatctagag gatatgaaaa
     2641 ataaaacctg gccatggatt gcaagcgtgc ctctaccaaa agatatctat gtagaaaagg
     2701 gtggacaaaa ttgcgaacaa gaaggagcac ctttttcttt gactggaccg accaaagaaa
     2761 taataggaga cgatggacaa acctatattt gtagcacaag cggaggtggt ggaccgtcaa
     2821 caatgaatcc tggtggagcg cctgtggctg gcactagaac acaatgtcct tttgattctt
     2881 attctcatgg tggagtttgg gcaatggact ggagagctga ttttggcaca ccaattttct
     2941 caactttttc gggtattgcc agactcagtg agggaaatgg ctatggtttt tatatcgata
     3001 ttcacagtaa ttataatggt gatgattttt ttattcgata tggtcatatg ccaccggggg
     3061 gatatcgggt cggtgacgga gaagcggtgg cagctggtca gcaaatagga attgttgata
     3121 atactgggtt ttcaagtggg aatcatttac actatgaagt agtgggagcg aatatagact
     3181 ggggcaatgc tggcccttat tttggaatta gtcaggaaga gtttaatagt ctttgccgat
     3241 agagttaaaa taaaatatgt ttataaaaaa taaatggata atagtaggaa tacttgtaat
     3301 aggattattt ttttggttag tgtggccaaa aaataacagt gaacctacaa ataaaccaga
     3361 agtaccggta gtagtcccaa ctttggagat agataatagt cagactattt ttccagaaac
     3421 attaaaaaaa gtcgaaactg tagtttggag tccagaaata aaacaagatt atcccagaga
     3481 actaaaaaaa ataattgtta aaagaaaaac ggttgatgaa aaaagagaag aagaaataat
     3541 aaaatatttt ggaattaaca agcaaaatgg ttatatatta aaaaagcaaa actatattca
     3601 gtatacaaat atgcccacca atttggataa agtaccggtg actacgaatt gggaaattgg
     3661 tgagattaaa aataaactaa ggaaaattgc aagtgattta aataaagaaa taggtctaga
     3721 agttgagtgg ataactacca gttatagaaa atataaccag ccatatatgg ttgaaacaac
     3781 tcaaaatgaa gcccaatttt tggaaataag tggtgattat ctagttgagg gaataagatt
     3841 gactactttt catggtgaag cgataaaagc ttattttgat ggccaaggaa acttgattaa
     3901 aataatgatt tatttaaagc cagagattaa taaaactgat aattactggc aaataatgac
     3961 actagaagag gcgatgaata gtccaataaa taattaccga gctggagtaa atgaccttta
     4021 tgatgaaatt aataaagtaa atttaaccca agcagaattg gtacaaattt atgataatga
     4081 aaaggaaagt attgctcctt attttttact ccagggaaat actttttcag caaaagaaaa
     4141 taaaccggtt aatattcccg tattattaaa ggcagataaa tgaattttga agatatatat
     4201 aaataccgat tgataattat cttgataata attgcgacgg caatgggagt agtaaagttt
     4261 aaatacagaa atgttaaatg ggaacaaaag gaaatgattt tgattgtcac tccaagtcca
     4321 accatttccc ctcaaatcaa tgttgagtat ccgttatggg aaaagttacc atacaaagga
     4381 acaggctacg ttgtggagcg ttacactgag ccaaacaccc ttttaataaa gatagacgga
     4441 gaggtaaacg aggaaataat tagccaagaa atatataact ggatgggaga aaataaagtg
     4501 gcgacagaaa gccataaact gatttttgaa gaaaattaaa gaggaaaaag tctaaattct
     4561 aaagtctaaa aaataagaat taatcttgaa tgcgaacggg ccagcctaca ctaaagcttc
     4621 ggcttggtaa acatgatgat gtgactaatc ttggatacgt acaggcatga tgatgtggat
     4681 gagggcggag tcagactgat cacggaaaag accaggagaa agagcttcgt ttaattcaat
     4741 aataatttct tcacctttac aaatagttaa aaaatcggaa ataaatttgt agttaaaagc
     4801 tatttctaag ggctcacctt caattcgagc atcaaccgaa actttgtttt gaccaatttg
     4861 aggagcatta gccgaaagtt caatcgagga agcttttaga cttaatttaa caacattggc
     4921 tgattgacgg gcaaaaacag agctttgttt aatggcctga gctagttcat cacgatttaa
     4981 gtaaactttt gtactggcat tttccgggat gatccgatga taatcgggat aatcaccctc
     5041 tatcagccga gagaccaatt caagatcctc taaaacaaag acaacctgat ttgagtcttt
     5101 agtcagtcca attttaattt ttggggcgtt tttagctaat tttacgattt cgagtaaact
     5161 tttggcggga attaagaggg tttgaatttc agaggaaaga ttgagggggc tcgtaagctt
     5221 tatatttttt aaagacaatc taaaaccgtc ggtcgccact aaattcaact cagtagaaga
     5281 aaaagtacaa agaatcgcag ttaagatggg acgggaatcg tcggtggcgg cggcaaaagc
     5341 tatttgatta atcgcctggg ttaaaatagg taaatcgatt tcaaaggcag ttttaggatc
     5401 aagggaggga atctgaggaa agtcagccga aggcagacag gcaaagtttg attcagtttt
     5461 gtttgaagat agggttaaaa tattgttatt tttgagactg agatcgagac ggccggcggg
     5521 tagataagaa acgaattcag taatttcctt ggcgggaaga cataaagaac cttcctcttc
     5581 tattttggcg ccaagccagt aattaatgcc aagctctaag ttggtggctg ataattttaa
     5641 acgacctttg tcagtcgaaa aaagaatgtg atttaagata ggaagttgaa ttttggaagc
     5701 aacaaaacga gaggtgtgac taagagcgag agaaatattt tcttgtaaaa gggaaagctt
     5761 cattgaccta tatattaaca gattggagat ttagaaaaca gtagtattgt taatagagcc
     5821 aggggattag tggaatagtc aaatggatta gtagtaaaac gggaaatact taaataattt
     5881 gatagtgaat atgtaaaaag tggcggaaag atatgattta taaagatatt ttagcttata
     5941 gtaagcgagt ggggtttggg aaagtttcgg tgggaaaaag ggagaaaaaa gtgaataagt
     6001 cggtggataa cttgcttaaa aatgtggata agtttagagg gaatttttta tttggttaat
     6061 aagttctttt aagggtggag aagtttgaag gtcggcggca actttgtccc gggcatgcat
     6121 aatactggtg tgatcacgac caccaagcag ctcccctact tctttgagag gtagtttgat
     6181 ttcggttaaa agaagataag cggtgatatg acgagcagta actagttctt ttttgcgaga
     6241 tgagccacat aaatcccccg ttttaaggtt gaaatatttg gcgcagaccg aaagaagatg
     6301 gcgggcattg agccttggag aggaggaaga aaaagcggaa ctaagagaac cattttggga
     6361 agagaaatgt ttttgaacta aatcaatatc gacaagcttg tggttttggg ctaaagaaat
     6421 aacagccagc tcttgaattt taccctcaat atcgcggata tttgattgaa tattgtcggc
     6481 gagaaactga actgcttcga aggtcagatc aataccctta ttttgagcct tttgttgaat
     6541 aatggcgctg cgcatttcta aatctggagg ttgaatatca accgtgagtc cacccataaa
     6601 acgggaaacc agacggtctt cgagctcttt aatatcgccg ggtttacggt cagaagtgag
     6661 aataacctgc tttcccgcta aatgaagagc gttaaaggta tgaaaaaact cttcctgaat
     6721 atattcttta ccggctaaaa actgaatatc gtcaaccaaa atacaatcta actggcgata
     6781 tttatttttg aactgattaa tggctttggt tttgagagca ttaatgagtt cgttaccaaa
     6841 agtttcggcg gggaaatatt taatttttag atcgggattg tttttgagaa gctcgtggcc
     6901 aatggcttgc atcaaatggg ttttgccgac cccgactcca ccccagagaa aaaagggatt
     6961 gtaggaaaga ccagggtttt taattactcc ttgggcggca gcataggcga aattattgct
     7021 attgccgacg acaagatttt cgaaagtata gtttggatgg agaccggcct gagtaaaatg
     7081 agtggcggag cttttaggtt gataggaaaa aagaggagaa gaagctagac cattactgtc
     7141 ttcattttta ataatttcct gaacgataaa tttaaggcga ttatcttttt tggtttggtt
     7201 gtcaaaggcg cttttaaaaa gttcataata gcgtttttgg tttaaatcac aaagataggc
     7261 attagaacag gcaataatag cctcagaatt ttgtagagaa atcagctgag ttttggcaac
     7321 gaaggtttgg tagacagcag gagcgagaac aacccggagc tcctcctgag tatttttcca
     7381 aagttggtca aggtccatat aatataaaaa aattctaaat tctaaattct aaaaaaataa
     7441 aaaataaaaa ataactaaga tcaggcagaa aaatggtctc gaggcaagag atcctttagg
     7501 gttatccaca ttataataaa ggagttttcc acagttgaaa agaggtcaaa ttgaagaaaa
     7561 taaaaaatct aaattctaaa acctaaaata agagaaaaga tgataaaatt accgcatgaa
     7621 acaaaagggg acatttcaac caaaaaagaa aaaaagagcc aaggttcatg gttttttaaa
     7681 aagaatggaa acagccaaag gacgaaagat aattaaaaag agaagagaaa aaggcagaat
     7741 aaagttgacg gtatannnnn nnnnnnnnnn nnnnnnnnnn nngagataaa aaacaaggga
     7801 gagatgaagg cggaggaatt attttcgata gtttggataa accctccttc gcctacggct
     7861 acggaagggc agaggaagtt tggagtgata gtatccaaaa agatttcgaa gaaagcggta
     7921 gagagaaatc aaattagacg gagattaatg gaggcagtga gactaaatat ggatattttc
     7981 ccagaaggat tcaggggaat ttttttagtg aaaaaaaata tattagataa aaaagaagga
     8041 gaaatagaag cgtgtttaaa aaaattattt taaaactttt agtgttttat aaaaaatact
     8101 tatctcgagg agaaaattgt cgttttgtgc cgacttgtag ccaatatacc tatcaggcgg
     8161 tagaaaaata cggagtggta aaaggtctgt ggctgggagt aaaaagaatt agtaaatgtc
     8221 atccatgggg cggtggcggt gtagatttga tagaatagaa gtcatgaata tttttattgc
     8281 gccttttgtt aatgctctgt ttgggtttta ttatttattt ggaaaccttg gctggtcgat
     8341 tattgcggtc actattatta ttagactggt attgatgcca ctggtttggc caagtttaaa
     8401 gtcggccaaa aaaatgcagg aactacagcc aaaattaaga aaattgcagg aaaaatacgg
     8461 aaaggataaa gagggattgg ctaaagcgca gatggaattt tacaaacaag agggggtgaa
     8521 tcccctgtcc gggtgtttac caaatatttt acagattgta gttctgatga ttttcttctc
     8581 agcttttaat atggtttccg gctttgctga gggaaaaatt acccgagaaa cggtcaacca
     8641 gcaactcatt caacagttta aggccagcga agagtttaaa tttagagaca catttttggg
     8701 aactaattta tcgatgactc cagctaaggc tctgggtggc ggaattagtc cggctttaat
     8761 tttgccttta tttttactgc taggatccgg tttacttcaa tattggagtg ctaaaaaaat
     8821 gatgccnnnn nnnnnnnnnn nnnnnnnnnn gaggatgata tgatggcagc gatgagaacc
     8881 cagtcgacct atatgatgcc actgatgaca attattatcg gttttaattt ttctattgga
     8941 attcttcttt actggtttgt taacagtgcg gtaatggcgg gtcagcaaat gttgatggcc
     9001 agaattgaca ggaaatagtg ataaaagttg tggtaaaatg gccactatgg ataacaaaaa
     9061 aagaaacgaa attatattaa atttgacctg tgaattgctg tcaaaaatca acttcgaggt
     9121 cgacaatgct tttgttgaag acgtggttgg agaagagggc caggtactcg taaatgtaac
     9181 tgtcaacaaa ccagggggat taataggttt tagaggacgc aatatggccg ctttgcagct
     9241 aattttgtct ttaatggtaa aaaaagaact gggtgaatgg gtgagagtcc tgctcgatat
     9301 taataattat cgaggagaac aaaaagaaag actggaaaat atggctaaag agctggcaga
     9361 taaagtccta aaaaccggag aagaggttca tttatcaaac atgagctctt atgaaaggag
     9421 aatctgtcat gtggtgttga ctgaaattga gggaatcacc agtgattcgg agggtgaggg
     9481 agaagagaga catattgtga taaggaaaaa acaagaaaag tctaaaatct aaagcctaaa
     9541 gtctaaagtc taaagagaac aaaatgtgat aggatagggc gtggagagaa ttaaaactag
     9601 tttgggtgat gtcttattat taatttggtt gattttaatt ccaacgcaat tgggaagaca
     9661 tttttggcta ggagaaagta gtgtggtggg gataagaatt gattatttgt ccataatttt
     9721 ttatctaact gatttggtat ggcttttatg ggtgataaag aaatcttcaa tcttcaatct
     9781 acagtcttca aacaaaaaga ataagtttaa aaaactaaat ttattaaatt ttaataattt
     9841 gattttgatt atgtttgtgg gggtaaacat agttttggca acggcaaggt gggtagcgct
     9901 ttaccgctgg ctccgaattg gacagtggtg gctgacggta aaattgctga gtaaaaataa
     9961 aaaaaaattt accaattatt taacaaaaat agtgccttgg tggattattg tagaagcctt
    10021 tttaggttta gctcagataa taaaaggtgg aagtctcaat ggaattttgt ggtggcttgg
    10081 ggagagaagg tttacttatg gaggcattgg aattgctcag tttagagttt ttgatcaggc
    10141 ctggataaga gcttacggga ctttttctca cccaaactct ctggctgggt ttttgctgtt
    10201 ggcgtggtgg tggtggaaga aacaattttc aatcttaaat cttcaatctt cagctgagaa
    10261 gatggggtac tggattgtaa attggagtgc ggtgctggga attgccttgt cgggaagtag
    10321 ggtggtgtgg gtggtgacgg cgggaatgct aatagcagaa gaaattctaa aaactaaagc
    10381 caaaagtcta aaaattatta cgggaaagat actactctct gttggagtta tttgtttaat
    10441 tttgggggtg gttagtatta actatcgaat cagtgacttt gtgggaggct gggatataaa
    10501 tagccttgaa aaacgagagg cactggcagt gtcggcgcta aaaatgattc gaaagtcccc
    10561 tctttttgga attggggcgg gaaattttgt ggttaatttg ccaaaatttc cgacagggaa
    10621 cttttactgg atgcagccgg tacacaatat tcttctactt gctttaagtg aaataggaat
    10681 agtgggaata attatattaa tcttcaatct acaatctacg attattaaaa taatgaaata
    10741 taaactttgg tggttgtggg cgattgtggg agtaacaggg ctttttgatc attactggct
    10801 gacgctgcca caaaattcat ggcttttggc gattatattg ggattgattt aaacctttcg
    10861 gccattctgg ccacctccct tgaagaaggg aggcaaatat aagataaaat aagtgatgtt
    10921 taaaagactg gcggtgaata ctggggtgca aattttgggt aaagcaatta tggttttggt
    10981 gtctctaata acaactggaa ttttgacaag aagactggga attgaggttt atgggcaata
    11041 tatttttatt acctcgatct tagtattttt tgatagtctc gccgattttg gaacttcgat
    11101 aatgggagtt agggaggcct caagaaagga aggtgaagag caagttaagg tttggaccaa
    11161 tactttgatt tcccgactga taatggcggg attaagttta gctttgggaa taatttttat
    11221 ttttttctgg aatgatttta gagaaattcg aatagaggca attctggcct gggtgatgat
    11281 tctgtttacc tcccttgctg gcagtttcgg tgttgtctgg caaacccgaa ttagaatgga
    11341 aataaaggtt ctcatcgaag tgatgtttcc gacaatattt ttgctctgtc tttggattta
    11401 tgggggaaat attagcttaa ggtggatttt tggaacttat ttatttgcca gaattgttac
    11461 cctgacctgg ggctggtggc taggcagggg aacaattaaa tttaaattag tagataagaa
    11521 gctgattagt aaaatattga aaataagctg gccaatgggg gtttatttgc taattttttc
    11581 tgcctatgat cgggccgttg atagcttaat gattcaaaga tacttagggg ccagagaagt
    11641 ggcctggtac ggcctcgcct acaaaatcta tggagtttta attcaaccgg cttatttttt
    11701 ggtaaacgga attttcccaa tattatcaaa aaaacaaaaa cagtctactt caaataatat
    11761 ttttaaaact tcggcgatta ttttattttt atcttcagta ttggtaataa taggagtctg
    11821 gctaatggca ccaatgatgg tatcaatact ggccggaagt gaatttggag cttcagtggg
    11881 agtgttgaga attttaattg tcgctctact ttttgcctat atgggacatt tagtgggatt
    11941 taccctgatt tcaaaagaag gacagaaaga aatgctgggt gttggtttaa tcattttaat
    12001 ttttaattta attggtaatt tggtggttat cccccgtcat ggaataatgg gcgcggccat
    12061 ggtaacggtg ttgacggaga tgttatcgtt aggactaatg accttaagac ttaaaagacg
    12121 ctggtaatta cgatttattg acctattccc ctacttaaga tactgacgga ttttttgatg
    12181 tctaaaatta aaatatatac attttttttg cataaatatt ttttaagaca ctctcacgtg
    12241 atggattacc tcaccggctc gtgcttaaaa aatacttata gcaaaaaata taaatacttt
    12301 ttgatctgta tcaatactca gttaattagt attcagttat gacgattttt cacgttttgt
    12361 agagatggtc tgacaagtta tgacagattt atcatagaaa taaccgcata ataaccgaaa
    12421 tataaccgca agggtaaaga tgctttagaa tagagacatg gaggcgttaa tctcggcggc
    12481 aattattttt ggaatagata actttgtggc ggtaaaactg gtctctaaac tgcgggagca
    12541 ggggataaaa ataattgatg gagaaattga ggaaattcct aaaaatgcgg catatgtttt
    12601 tgattttgaa ggcagagaga gggagtggga gagattggaa aaaaatcaaa agttagtttt
    12661 ggtgtcagac aatcaaaaac aggcagagga atggaaaaat aaacttgacg gtagtttcct
    12721 aaactggaga atagtttttg gggaaaatgt ttatggggag ggaatgagtg aagaagggtt
    12781 tttggggcag gcatttttaa ccgcggccag aaataaaaac ttgattttac cgagtcttga
    12841 tcaggtttac cgggttctgg cggtggagga ccttgtcgag gcaatactcc ggtcctgttt
    12901 tttttctggt actagtggaa aaattttctg tattggggga cgcgaaacga actcgaagat
    12961 agtggctgag gtgctcttag atgaggcgaa gatgacgaga actaaggtaa ttcaggattc
    13021 gtcaaaaaaa gaagagtcgc cggaaaagga aaaaatgatt agagaaagcc aacaaatact
    13081 gagatggcag gcggaaacga cttttaaaca aggagccaaa gaggcggtgc aatattttgt
    13141 agcgaaggtt gatgctgata accgaaaaaa ggcggcagtt aaaagcaacg attttaagcc
    13201 aaaaattgaa gaagaaaaga gagaaaggag atttgaggtg gagatagaat ccgacttcgt
    13261 cccatcagag aaggactacg tcgagacaaa gaaggaggaa attgagatag agattgagga
    13321 agaagttgaa gaaaaagtga aacccgccgt cgtcgaagac tttggcgaga caaaggaaga
    13381 aattgtgttt gaaaaatttg catcaagtcc gataccagag agaaaagtgg aagaagaaaa
    13441 agaggaaaaa cccgacttcg tcccgattaa gcgggacttc gtcgaggcga aggaaaaaaa
    13501 gaagaaagat aactggtgga ggtgggggct tggcgcggtt ttattgatat ttttggtggt
    13561 acttatcata aatataataa aagtggcgtc aattccaaaa aatattttga ggatttcaaa
    13621 cttgattgaa gaaaacaatt atgttgaagc tgaaaagaaa attaaaattc tggcctcaaa
    13681 caacgagaaa aatttagcta tttttgaaac aggaaaagta ggcagtctgc tacggattga
    13741 aagcgaagta attgaacttt taaagttaaa tctggaactc gtcaaaagca gcgagaaaat
    13801 tagtcaagat atttttgggg agacagaggt agaaatggaa gaagagcttt tcgaagtggc
    13861 aaaaaagaca gaaatggtga tttctaaaat ggggattttg caagggagac tgtcggggca
    13921 gtggggctgg ctcccgagta gatttagaag cgatctaaat aaaattaaag taaagctgga
    13981 aagccaaaga aaaacagcgg aaaagatgaa tgaaatactc cggatactac cggaaatgat
    14041 tggacttgat ggaaaaagga gagaatacct ggtgctcctg caaaacgaaa acgaaattag
    14101 gccatcaggg ggctttatcg gttcttttgc gattttggcc tttgagggag gtcgttttat
    14161 aaattttcag gtcaatgata tttacgaagc agacggacaa ctaagagggc acgtggaacc
    14221 accagaggaa attaaaaaat atttgggaga ggcgggctgg tatatgagag atgctaattg
    14281 gcaagccagt tttcctctgg tttccagaga tgtccaatgg tttcttgata aggaaacagg
    14341 gaggaaggtt gacggagtca tcggattaaa tttggcttcg gttaaggcgg tacttggagt
    14401 aatgggggaa atattcgtgc ccgattttaa ggaaaaagtg aatcaaaata atttatatga
    14461 gcaagccgag ttttattcag ataataaatt ttttgcgggc tcgggacaaa aggcgagttt
    14521 tctcggagga gttagtaagc aactaatgga agaaattaaa atggccaagg gaaatgtggg
    14581 acaaaaacta ctgacgacaa tagttgattt acttgatcga aacgagatac aaattagttt
    14641 taacaatagt gaagcggcca aggtgatagc ggaggcaggc tgggacgggt caatttacga
    14701 gggaaaatgc ccttcgacaa gctcagagca ggttggttgt tttgcggact atttatatct
    14761 agtagaggca aatatgggtg taaataaggc aaattacttt ttgtatcgaa atgttgaaag
    14821 agaggtagaa ataggagaaa agataattaa aaataaatta aaaataattt atgaaaacac
    14881 ttctaaaagt accaactggc cagctggaga ttataaaaat tatttaagag tttacatacc
    14941 gaccgaaagc cgagtagaag agattaattg gagtgagagt ggcagtggcg aaaaaaatat
    15001 tgtcagtggg gagaacctaa aaataaattt gaacggtagc aaaagagaag tgggttttct
    15061 ggtgactgtg cctattggta agaaaattga agttgaagtt aaatatagtg ctgaaattga
    15121 tttaagcaaa gtgacaactt tttcttattt aaattatgtt caaaaacaga gtggttatgg
    15181 agagacaaat atggtgagtt tgatttcagt gcctactaat tggcagataa atgcggctga
    15241 gccggtggcg agtgtcgtcg gaggaaaact actttttaat caaaagctgg agaaggatat
    15301 taaaatggga gtagagatag agagatgaaa aattttataa gaaaaactga aaattttgag
    15361 tgtgaaaatt gcggggaaaa ggtgatgggg gatgggtata cggatcactg tccgaggtgt
    15421 ctttttggga aacacgtcga tgaagaaatt cccggagatc gggcgagtga ttgtgggggt
    15481 ctaatggaac cgattaaaag tgtttataaa tcgggatatt ttaaaatatt ttataaatgt
    15541 acaaagtgtc gacatgagtt ttgggtaaaa gccgccgacg ccaaggctat ggcaggccaa
    15601 gggaggggtc aaggggatga cagagaaagg ttgatagaac ttttggggaa ataaaaaaaa
    15661 ctaaagtcta aaatctaaat aatataaatg gtggtgatgt tataattgat atctatgtgg
    15721 ggaagaataa aaaagatact taagaaaaaa gataacaaac cgacttcgcc gaaagctacg
    15781 tcgagtcaag ggataccaaa agagataatg gataaactac caccgggagt aaaaataaaa
    15841 agaatcgaga taggtccggc acaaataatg aagaccttaa tttctctatt gatgttttac
    15901 ataattggct cggctctaat cacgggtctc gtcggagata aactggcaaa agtaccccta
    15961 tcaagggtta ttgaatcgat aaaaaagaat gaggttaaag aaataacggt tatggacaat
    16021 gaaattttgg tgacactaaa agacccgact tcgccagagg ctacgacagg gcaggcgaaa
    16081 atactggtag cctccaaaga aagtaatgtt tcaatgtcgg agataatgca aagagaggga
    16141 gtagatatgt cgtcggtaag ctttaatgtt gaaaacagaa gtggctggaa agccattggg
    16201 gaaatactgg ggacactact cggaattgga attccgattt tgtttatttt gtggtttttt
    16261 aatcgacaaa gcggggctca gggtggagga atgtttggtt ttggaaaatc aaccgcaaaa
    16321 ctatttatca agggaaaaca aaatattact tttaaagatg tggccggagt ggaagaggcg
    16381 aaaagagatc tggaagaagt ggtcgacttt ttaaaacatc cagaaaagta tcaaaaaatg
    16441 ggagccaggg ctccaagagg agttttgctg gtaggaccgg caggagttgg taaaacactg
    16501 ctggctaagg cggtagcggg agaggccaat gtaccttttt atagtatggc tgggtcggaa
    16561 tttatggaaa tgctggtggg agttggagcg agccgggtac gggatctttt tgggacggcc
    16621 aaaaaagcgg gtaaagccat tatttttatt gatgagattg atgcgattgg acgaatgaga
    16681 ggaggaatgg atggaggcca cggagaaaga gaacagacgc taaatcaaat actagtcgaa
    16741 atggatggat ttgaaactaa tacggcggtg gtagtattgg cggcgactaa tcgcggcgat
    16801 ttactagatc cggcactact gcgaccaggc cgttttgaca ggagagtggt cctcgaaatg
    16861 cccgacttaa aggacagaga agagataatt aaaattcatg ctaaagggaa accttttgaa
    16921 aaaaaggtga actggacaac tgtggccagg agaacggtgg gtttttcggg agctgatctt
    16981 gaaaacatgc ttaatgaggc ggctataaaa gccgccagag aaaataaaaa agaggttgaa
    17041 atggtggata ttgaagaagc ggccctaaaa gttaaactag gatcagaaaa acgccgagat
    17101 caaacagaag aggataagtt aatgacagct tatcatgaag cgggacacgc gattgttaat
    17161 tttgctgagg acctagaccc ggtacaccga atttcgattg tttcacgggg aatggcactc
    17221 ggatttaccc taattccgcc taaaatggat agagttcacg aaactaaatc gagactgata
    17281 aaacaaatgg cgatggccat gggaggcagg gcggccgaag cgctggtttt taacgatatt
    17341 accaccggag ccagcagtga tattaatcac gtgactaata ttgccagaaa tatggtggtc
    17401 gagtggggaa tgtcggaact cggacctata aattggggac cacaaattga taccgatgat
    17461 tttgggaaag cttggatgga gccgagtaaa atttctgatg agatgcaggg aagggtagat
    17521 ggggaaatta aaaaactagt caatacggcc ttaaataggg ccgaagaact acttaagaaa
    17581 aatagaaaga aattagatga actggctaaa cttttagtgg aaaaggaaag tttggatgac
    17641 aaagaattcg aagagtttat gaa
//
LOCUS       DHGB01000052           24378 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_87068, whole genome shotgun sequence.
ACCESSION   DHGB01000052 DHGB01000000
VERSION     DHGB01000052.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 24378)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24378)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24378
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_87068"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         6893 a         5147 c         4062 g         8270 t
ORIGIN      
        1 ttaccttggg tgatctagtt atggtctcgg gttttattac tggtttttat tatcagtttt
       61 ttaatctttt tttccggatt cgtgatattg ccaaaaatgt tactgatctt gataaatatt
      121 tttctattct tgaaaatgaa accgaggtta aagatccctc ttcaccaaaa actttatctc
      181 gccccactgg ttctcttcag ttcaaaaata tcgcttttgc ctatcctggc aataaggaga
      241 gggtagtcga taaaattaat ctcaaaatta aatcaggcga gcaagtcgct cttgttggtc
      301 gttccggtgc cggcaaaacc actttagtta aattactttt acgtttttat gatcctactt
      361 ctggtgtcat caaatttgac ggagtcgata tcacccactt taccaaaagc tatttgcgta
      421 gcctcatggc cgtcgttccc caggagccaa ttatgtttaa caacacgatt aaatttaacc
      481 taagttatgg caaagaaaaa gccaagatga gcgaaattag aaaagccgcc gccgatgcca
      541 atattttaac ttttattgaa aatctacccc aaaaatggca aaccgaagtc ggtgagcggg
      601 ggataaaact tagtggtggg caaaaacaac gcctggctat cgccagggca cttttaacta
      661 atcccaaaat tttgattttt gatgaagcta ccagtaattt agattccgag tctgaacgtc
      721 aaattcaaaa agctctcaaa attgcctctc aaagccgcac cgtgattatc atcgctcatc
      781 gtttttctac catcagaaat gccgataaga ttgtggtttt aagtaatggt atcattgctg
      841 aaattggtaa acaccgtgag ctgattgcca aaaatggtct ttacaaaatg ctctggaccc
      901 ttcagtcaaa gggcaaatta attactgata aagaaagttt attcgattct aatataagat
      961 aagggattta aatgttaaaa taatcccatg gataagagtt attcctccgc cactgaagcc
     1021 gatatttatc gttcttggga aaaatccggg gcctttaccc ccaaaatcga taagagcaaa
     1081 accccttttt ccattgtttt acctcttccc aatgctagcg accccatgca catgggccat
     1141 gctcttttta ctattcagga cattttagtt cgttaccatc gcatgctggg tgaccctact
     1201 ctttggcttc ccggcgccga tcatgccggt attgaaactc aatttgtttt tgaaaaaaaa
     1261 ttgcaaaaag aaggcaaatc tcgttttgat tatgatcgtc aaactttata taaaatgatt
     1321 tgggattttg tcgaagaaaa tcgaatctta aaccaatttc aaatgaaaaa gctgggcttt
     1381 tccatggatt ggacccgtta tcactacagt ctcgaaccta aaattgttga caatgtcctc
     1441 ctcactttca aaaagcttta cgacgacggt ctcatttatc gtgacgaaaa aattgtcaat
     1501 tattgcactc actgtggtac tgctttttcc aatttagaag tggttcataa aactgtttat
     1561 tcccaccttt ggtatataaa atatggtctt ctcaccgtcg ccactacccg tcccgaaacc
     1621 atgctgggtg ataccgctat cgccgttaat cccaaagata agcgttataa aaatctgatt
     1681 ggtaaaaaaa ttctcttgcc cttagttaat cgggaaatac ccataatagg ggaagaaagc
     1741 attgatatca aatttggtac cggggctgtc aaagtgactc cttctcattc tcccgaggat
     1801 tatgatatcg ccaaaaaaca caatttggaa tttatccgta tttttgatta tgacggtaaa
     1861 tccaatctca atgtccccga aaaatataga ggttatttcc catctcaggt ccgtcaaatg
     1921 gtgattgatg atctcactgc cgtcggtcta atcgaaaaaa ttgaagatta ttcccacgaa
     1981 gtcggtcact gttatcgttg tggttgtcct cttgaaccca ttaccgctcc tcagtggtat
     2041 cttagaattg ctccattagc taaacctgct attgaagccg cccaaaacgg tgatgtcaaa
     2101 ttttttccct cccgtttcaa aaaagcttac cttacctgga tggaatccat tcgcgattgg
     2161 aatatttccc gtcaaattgt ttggggtccc cgtattcccg cctggtactg cttagattgt
     2221 catcccgaca ttaaactaaa ctttttagat aaaaataaaa atattgttac cggcttttat
     2281 aaagatctaa aagacaaata ttcttttgag gaaataaaaa atggtctgca atccctgata
     2341 gcaccggttg acgcaaaata tattgtagat tgtagttcga agattgaaaa ttgtcccaac
     2401 tgtcattcca cttcactttt acaggaaacg gatacctttg acacctggtt tttgagcggt
     2461 caatggcctt taaatactct cggttttaat cccaaagatc caagcaaatc ctctcccgac
     2521 tttgcctatt tttatcccac ctcggttatg gatactctct gggatattct ctttttctgg
     2581 gtggcgcgga tgattatgtt tggcctttat ttgaccaaag aagtgccttt taaaaccgtc
     2641 catttacatt cccgggttgt tgatgccaaa ggtcaaaaaa tgtccaaatc aaaaggcaat
     2701 gttattgatc ccttagtcat gaccgaaaaa tatggcaccg acgccctacg tatgtcactc
     2761 gtttacggta ttgctccggc ttctgatttt gtggtttccg aagataaaat tagggctcag
     2821 cgtaattttg tcaataaaat ttggaacgcc tcccgcttcg tcttaatggt agtcgacaag
     2881 tttcccaaag taggggcttc gcttgccgga gcccaaatta caaaccccga tgatatctcc
     2941 attctcacca atctcaataa aatcatcacc tctactactt ccgatattaa tcgctatcat
     3001 tttggtcaag cttcagaaaa tctttatcaa tttttctggc atgatttttg cgatatctat
     3061 attgaaaaag ccaaagatag gcttgacccg tcgaagccga caggcgaagt cggtgaggaa
     3121 gatgtcgtcc cggttctgct cgctgtttta attacctctc tcaaacttct ccatcctttt
     3181 attccctttg tcaccgaatc tgtttaccaa atcctccgcg aaaaattgaa cttagacgaa
     3241 gctcaactaa tcactgcttc ttggccagaa gttctttcat tttaactaaa tttttggcca
     3301 tttccggttt ctcaaaatat gttctggctg gttccagttt gttaattagc cactccgtta
     3361 gggcattttt taaatcaagt gggaataatt ctcccgaggc ataatcagtc actatatcgg
     3421 tgtatctgcg ataagtttta ttcccaccaa atttttctgg tctttcaatt gtaaattctc
     3481 ccgtctcttc accccaaaaa accagggttt ttacccagtt taggagagga ttaaattcaa
     3541 ctattttagg cggcccataa gctttcaaga ttttattttt tatttcctct ggcgtgtcat
     3601 gaataaaaat agccgaattt ggtttcgatt tgctcatttt tagcgattct tcatctttat
     3661 tatctgtatc tgggcttgtt aatcccgcaa ttaaattttg atgaattgct actggtgttt
     3721 gaaaatttcc gggaattttt ttaatagcgt ctctggcaat cacatgagcc tttctttgat
     3781 ccatgccagc gtgcgctaaa ttaatattca tcgtcgctat gtccgccacc tgcagtggtg
     3841 gataaaacaa agtcgccata tcaacctcat tacctagctt tttgcccatt atggaaatac
     3901 ttctcagatt tcttgacagt gtcacttgtt ttccaatctc cataaaattt tgccaatgaa
     3961 tcggattttt ttcatacaaa tcttttccac tgataaattc cacctggctt tcatcaaccc
     4021 caaaacattt taacgaggca atcaaagcct gcttaaaata attgtccgaa gcccagcgga
     4081 tatcttccca ttttccgccc agtttattat ttaaaaagct atgaaaatca gctaaaaata
     4141 ttttgcaatt aatcccggct tttaaaaaat cggcaatttt tcccatagac attaatcccg
     4201 ttcccaaatg caccaatccc gaaatttcaa agccaatgta gtgattaatc ggctttccgc
     4261 tctcaattaa tttttttaaa tcatcttccg tgagcacctc ttggcagttg cgggtgatta
     4321 aattcatctt ttcttctgta gtcatgcttc tattttctca tatttttaca tataattact
     4381 aatttttaat tgatgattga agaatgaaga ttgaagattg ataattaact tatgtcgaca
     4441 atttatcttt ttcgtcacgg tcagtcggaa tttaatttaa gtaaaacctt taccggttgg
     4501 attgatgcca aactgactcc actgggaatt gagcaggcca aagccctggg agagatgtta
     4561 aaagataaaa ccattgattt agcctatagc tccagtcttt ccagagctat cgacactctt
     4621 aaagaagttt taatttacca tcccgaatgc cgcgaaacca ttatcgatga ccgtatcatc
     4681 gaaagatctt atggtgatct cagtggccac actcacgctg aaactattgc tcaatatggc
     4741 caagaacaat ttgataaatg gcaccggggt tggaccgaca aagctaacaa tggtgaatct
     4801 tttgccgatg ttgaacttcg tgtccaatca tttattgatg atcttaaagc caaatatagt
     4861 ggtcaaaatt tgggtcttgc catcagtgct cacggtaatt ctatccgcct tttccgaaaa
     4921 attatagaaa atgctaccat tggagaaaca gtttcttgga ctattccata cgatcagtac
     4981 tttgaataca gaatttaatc ttcaatcttc aaacttcaat cttcatttta tttaaaaata
     5041 cccttttgtc ttaaagtcga ttgaattttg cctataattc tcacaatttc atcacaacta
     5101 ttaatcaacg attcagactc attcttctca agtaaattac tgtcttttaa cagtctaatc
     5161 cagtagttag tttctcttgc ttctttataa gcaattgata attttgaata gaaatctttt
     5221 tctgattgtc caccaattga ttcttctacg tttgctccaa ttgaagtacc acttctaagt
     5281 agttgtttag ataatacata ttcttttttg tttatagata aatttctata agttttaatt
     5341 atttccaaag ccaaaatata acttttgtct cttaagatat tattcgccac ataatcaatg
     5401 taacatatct aacaaataat tgttaattgt ggattgaaga ttgtagattg aagattaccc
     5461 caactcttct tcgattctca tcagtcggtt atatttacac actctttcgc ctctagttgg
     5521 gccaactttg atatattcac tgcccacggc cacggccagg tccaccataa aagtatcatt
     5581 tgattcgcca ccgccccggt gagagggcac tgtccacata ctattttcct tagcctcaat
     5641 acagcaatca atggtttcac tcaccgtccc tgcctgattc agtttaatca aaattgcatt
     5701 tgcggctttc atttcaatcg ctttttgcca tctttttagg tttgtcactg tcaaatcatc
     5761 agcaatatta ggcatatttc cgactttact ggttagttca acccagtttt cccagtcatc
     5821 ttcagccagc atatcttcaa agctgacaat cggatatttt tgtagccagg gcaaaaaata
     5881 ctcaatcaat tcttttccgc ttaacttttt attttctatt gttagattgt aggttgaaga
     5941 ttgaagatta aagaattctg acgctgctgc atcaatcgaa actccagcat caattcccgg
     6001 gatatagccg gcatttttaa tcgcctccac tagatattcg atgggttttt catttgaagt
     6061 taatccgttt ggagcaaaag ccccctcatt tccgacatta gtcgatttat tctctttttt
     6121 aagaattttc tttaactctt catagatttc catttccatt ctcacgtttt cggcggcact
     6181 tctgtttccc atggctgaga ctaaatattc ttgtaaatca gtactttcag ctgcgtgctt
     6241 tcctccttca atcattacca ccattggttt gggtaaagta tttaatggaa tcaatccgta
     6301 tattttttgc aggtatttat aaagaggcaa ctcctcctct gccgcctggg ccctggccac
     6361 cgccatactg actccgagga ttgcatttcc ccctaaatta gacttatttt ctgtcccatc
     6421 taggtcaatc atcaatttat ctagaattct ttgttctccg ccttccagtc ccaaaagttt
     6481 tggtccaatt atttcattaa tattttttac ggccttaagc attccttttc cgccatatct
     6541 cttttcatct ccatctaaaa gcacggtggc ttcatgactc ccggcgctag ctccataact
     6601 aaccgcagct tcaccgacgg ctccagattc taatttcacg gtaacttcga gtgttggtgc
     6661 cccgcctgag gccaatattt ctctggcttt aatttgtttt attttcgaca tataaaccca
     6721 ttataccctt aatccatttt ttataattaa tatttttgat cattataaaa taatctttta
     6781 atttcatttt cttttttaaa aataaatcgc agtaactcgg cccatagatt tttattcctt
     6841 tatatatagg ccaggggaca gaagcaaagt cattaatact ccaaaaattt tgataatttt
     6901 ttttcaaaaa atttatcatt ttattgtccc gatattcacc cggtgtgtat tccaaaaata
     6961 tcgtcggctg gtctcttttt attatttttt ttgttccact tatcacctcc ggctcccatc
     7021 cttgagtatc gaccttaatc aaactaatat attgttcatt tatgagcaaa ttatccagag
     7081 tctccgtcat aattctagtg tctactttct cgtttctcgt ctctgatatc cgactatttc
     7141 ccaaattttc cccatccctt actaaatatt ttttctccct tttatcactc gctgccttgt
     7201 tgattagcac cacatttttt aaattatttt ccttgacatt ttttttcaaa atatcaaaac
     7261 attctttgtc tggctcaatc gcataaactt ttttacactt cttcgccatt aggatggtat
     7321 agtagccaat gttggctccc acatccacaa cgacactatt tatattaagc tggccaagca
     7381 ttaatgctgt ctcatatctt tcatattttt ggagagcaat tctctgacca atatatttat
     7441 catcctccct ataccacaaa tccaaattgt caatttttgt cttttttaat ctcatatttt
     7501 ttgtttttag attttagact tttttttctc tctccattat ctcattaccc ccttgacctg
     7561 tggccactaa tggatataat attagcagtc tattgccccg actgataatt ataaattaat
     7621 tttattatgc ctaaatcaaa tatcaaacca gtagccggtt atcttttagt cgaaccctta
     7681 aagcaggaaa aaaccactgc ttcgggcatt gttttacccg aatctcacga ggaaaaacct
     7741 caatcaggtc gggtcattgc cgttggtggt cctatttttg acgacggtcg cgaaatatta
     7801 tgtcccgtta aagttggcga tgttattgtt tataaagaat ggggtggtaa agaatacaaa
     7861 gacggtgata ccaatttact tatcctcaaa tttgaggatg tcatggcaac aatcaattaa
     7921 attatttatt aaaattatca attttcaatc tacaatcttg aagtctgaag attgaagatt
     7981 tagaaattaa aaaaatatgg ctaaacaact aaaattcggg caagatgccc gccaatcatt
     8041 acttagaggt gttaatacct tagccaatgc tgtggctacc actctcggtc ctaaaggtcg
     8101 aaatgtggct atcgaaaaaa agtggggtgg tccctcagtc attcatgacg gtgtttctgt
     8161 cgctaaagaa attgacctca aagatcccta tgaaaatatg ggagcccagc ttattaaaga
     8221 agccgcttcc aaaaccaatg acaccgccgg tgacggcaca actacggcta ctgttttagc
     8281 ccaagctatc gttaataaag gtcttcaaaa tgtcgctgcc ggtgccaatc ccatgattgt
     8341 tcgccgtggt cttgaaaaag gcctcgccgt cgttatcaaa gagctaaatt cgatgaaaaa
     8401 agacatcaaa attgatgatc tggcttctat cgaaaaagtc gctacaattt cctctgccag
     8461 cgaagaaatt ggtcacgcta ttgccaccgc cgttatcaaa gtcggtcgca acggcctgat
     8521 taccgccgaa gagggcaagg gtctagacct cgaaaccaaa gaaactactg gtatggaatt
     8581 tgacaatggt tttctttctc cctattttgc caccaatact gaaaaaatgg aggccgtcat
     8641 tgagcatcct tacatcgtta tcactgataa aaaaatctcc tccattcagg atattttgcc
     8701 ctttttggaa aaattagtta aattaaccaa aaactttgtc attatttctg aggatattga
     8761 tggtgaagct ctggccactt tagtcgtcaa caaacttcgc ggtaccttca atgttcttgc
     8821 ggttaaagct cctggttttg gtgatcgtcg caaagctatg ctcgaagata ttgccatcct
     8881 aaccggtgga aaagttatcg ccgaagatct cgggacaaaa tttgaaaata ttgaccctga
     8941 ggagtattgt ggccgggctg aatcggtcac tgccgataaa gataaaactc aaataatcag
     9001 tggagccggt tccaaggctg aaattgattc gagagtggct caaattaaag tccagcttga
     9061 tgcctctact tctgattacg acaaagattc cttccgtcag cgcattgcga agttgattgg
     9121 cggggccatc gtcattcagg ttggcggagc caccgaagtt gagatgaaag aaagacttga
     9181 acgagttaaa gacgctattg atgccaccaa ggcggctgtt gaagagggta ttctccccgg
     9241 tggcggagtg tctcttctca aagcttcttt cgccctagac aaggtaaaaa cagattcatc
     9301 cgaggagcaa gttggcattg atattttaaa atttgctctc gagcaaccca ttcgaaaact
     9361 agccattaac tgtggtgaag accctggttt tgttttcaac cgaattaagg attccctcga
     9421 gtctgatgtt aaatcagatc ttggttatca cgcagttacc ggagagttta tttctatgac
     9481 caaagccggc attctcgatc cggtcaaggt caccaaatca gctctcacca acgccgtttc
     9541 agttgggact atgattttga cgactgatgt tctagtgtcc gatattcccg agaaaaaacc
     9601 cgcttctcct gacatgagtg gcatgggcgg gatgggtggt atggacggca tgatgtaaaa
     9661 tcaacctatg aaaaaatttg tctatttttt tggtcacaaa caaaccgaag gtaatgcctc
     9721 ggagaaatat attttaggcg gaaaaggagc caatctggcc gaaatgagta agattggtat
     9781 tccagtcccc ccaggattta ccatttccac agaggtttgc aaatattttt acgataataa
     9841 taaaaaatat cccgagactc taaaaaaaga agtcgaggca aatttaaaaa aactagaaac
     9901 cgccaccggc aaaaaattcg gtgatagcaa aaatccacta ctggtttccg ttcgttctgg
     9961 tgccgcctct tccatgccag ggatgatgga tactattctc aatcttggtt taaatgaaga
    10021 tgtagttaaa agtattgcca gttctaccgc taatcctcgt tttgcctggg attcctatcg
    10081 tcgttttatc caaatgtacg gcaatgttgt cgaaaatatc gaacatgaaa aatttgaaca
    10141 tcttattgac agtatcaaag acaaaaaggg aattagagaa gacactgata tgggtgctac
    10201 tgactgggaa gaagttgttc gggcttataa aaaattattt aaaattgaaa aaaaccggga
    10261 atttcctgat gatgcccagg ttcagctttg gggtgccatt ggtgctgtca ttggttcttg
    10321 gaacaatgcc cgggcgatta cttatcgtcg tttaaatcat attgacgaga ccaaggtttt
    10381 tggcaccgct attaacgttc agtccatggt ctttggtaat cttggggctg attctggtac
    10441 tggagttgcc ttcactcgaa atccggccac tggtgaaaaa aaatcctatg gcgaatatct
    10501 gatgaatgct cagggtgaag atgtcgtggc tggtattcgg actccaaaac atcttgactc
    10561 cctaagggaa gacaacgata aagtttatga tcagcttatt gctatttttt ccaaacttga
    10621 aaaacattat cgggatatgc aggatgttga atttaccatt gaaaacggaa aactttatat
    10681 tctccagaca cgtagcggca aaagaactgc ccctgcctct atcaaagttg ctattgattt
    10741 agtttctgaa aaattaatta ccaaaaaaga ggctctgctt cgtattgaac cagaaaaatt
    10801 agatcagctt cttcatccaa ctctcgatcc taaggctaaa tctaaatata agcttattgc
    10861 tactggctta cctgcttcgc caggtgccgg agttggtgag gtcgttttta atgccgagac
    10921 tgccgttgaa tggaaagaag ccggcaaaaa agtgatttta gtcagagctg aaacttctcc
    10981 tgaggacatt tctggcatga atgctgcgca gggtatttta accgcccgtg gtggcatgac
    11041 ttctcacgct gctgtcgttg cccgtggcat gggcaaatgc tgtgtttctg gctgtgaatc
    11101 cattactgtt tccgagattg aaaaaaaatt ccgtatcggt gacctaattg tttccgaagg
    11161 tgatttaatc actcttgatg gttcaaccgg agaggttttt ttaggagagg tacctgccgt
    11221 taatccggag ctttctggct attttggcac tattatggaa tggtcggata aatacaaaaa
    11281 gctaggtgtt cggactaacg ctgacacccc gagggatgcc caggttgccc gtgattttgg
    11341 tgccactggt atcggtcttt gtcgtactga acacatgttt tttcaggccg atcgcatttt
    11401 tgctgtccgt caaatgattt tagcggacac tgccgagcaa agaaaagtag ctctggccaa
    11461 aattttcccc atgcaaaaaa atgattttct cgaactcttt aaaatcatga aaggttttcc
    11521 ggtcaaaatt cgtttactcg atccacccct tcatgagttt ttacccaagg aaaaagctga
    11581 tatcgaggcc ctggctatgg aaatgggtat caccgtagaa aaattacagg ataaaattga
    11641 ttctcttcat gagcaaaatc ctatgatggg tcatcgtggt tgtcgtttag gtataagctt
    11701 tcctgaaatt ttcgaaatgc aggtcagggc tattgcagag gctgcctgcg aagttattaa
    11761 aaaaaataac gtcattgcga gcgaagcgaa gcaatctctt attcccgaaa ttatgattcc
    11821 cttaatcggt gaatttaatg agttcaaatg gctcaaagac agattagttc cggtaatcga
    11881 agacaccatt aaaaaatatg gcattaaaat gacttatgaa atcggaacta tgattgaaat
    11941 ccctcgagct gccctcaccg ctgatgccat tgctaccgaa gctgactttt tttcctttgg
    12001 cactaatgac ttaactcaaa tgacctacgg tttttctcgg gatgatgctg gtaaattttt
    12061 aaaagattat actgataaaa aaattcttgc tgaagatcct ttcaaaactc ttgacacagt
    12121 cggagtaggg aggttgatga aaatggccgt tgatcttggc cgaaacacca aaaagaatct
    12181 ccacattggt atttgcggtg aacagggtgg tgatccggca actatcaaat actgccacca
    12241 aataggttta aattacgtat cttgcagtcc ctaccgggtg cccattgccc gcctcgctgc
    12301 cgcccaggcc gcgattgagg cagagaaata gtaaaagaaa aattaaaagc tcccgatttc
    12361 tcgggagctt tttttgtata tatattaaat atatttttta tttattatct aaatcagttg
    12421 actctatgac ttcactagta gggtaccaag cagtacagtt ttcactttct aattgaatta
    12481 tcggttgtat attaggttcg ttatagcata catttctatc agtagatttg tcataataac
    12541 aagaactttt tattgatggt aaagtcaaag tacttttatc cattacttta tagactgtcc
    12601 cagattccat tacacaagta tctttaggaa tttgtatttg attgtaatca attacacctt
    12661 ccatgctatt tgtaactact ccggtatctc ctatttcagg atttattgtt tcattatcac
    12721 agccagctaa tagtaagcca ccaccaatag caagaccagc taaaaaagct cttgaagata
    12781 tatttttttc actcatattc gtatattaat taataataac tatacaaaat tattatccca
    12841 taatattagt tatttgtcaa gtagtataaa tataggatac tttatgttaa gatatccgag
    12901 tggaacgagt tattttatct aataaaagct actttaattt tagtttgcgt tttgaagaag
    12961 gtgatttacc aaaaattcta ttttgccaaa attgcccctt ttctcaaaat gcatgttcta
    13021 ttcttgactc tagtgtttcc aatcgaattg ttggcaaaat taatctaaac caccctcaag
    13081 tgttagagct atctagaaaa aatcttaaac ctacctgtgg tgatataagt gatttattga
    13141 aataaaacat taaagtctgg cggcagagct aaaaatagtt taaaatcacg cataaccgat
    13201 tcaatgaccc ggtagtcaag cggttagaca gcggtctgca aaaccgttca ggcgggttca
    13261 actcccgccc gggtctcaaa acaatttggn nagctttagc gtaggctggc cgcccgcgac
    13321 tcaaaaataa ttcttaagtt tttttatttc ctcttcgtag actttttgtg cccatttaaa
    13381 ttcccagtcg ctatgatctt gtcctggtag caccagataa ttttctcctt ttgactgtac
    13441 aatctcttta aactcatttc tcgaagcctc gcttatatat ttatcacttt ctccaaaaac
    13501 caaatgaatt ccaccttgat aattcattaa acattccttc acttttgatt cttttacttc
    13561 atctaaaaat tttcctttaa attgttgacc tttttgttcg taaattttct cagggtcaac
    13621 atcattccga ccttcaaaat cgcccggtgc ccataaaagt aaacatttaa ttttatcttt
    13681 aagttcagct gcacttgctg ttacaatggc actagccccg ccgagactat gccccaataa
    13741 cgcaattttg tagcctaatt gaaaatattt taaggcaaaa gatttgcttg tttctaccca
    13801 ttctttaaaa gaagactcaa gataattacc ctctgaattg gcactatttg cttgatcaaa
    13861 tcttaaaacc gaataaccct ctgctactag caaccgacta aaatcgacaa aagttctggc
    13921 tggtccgata ttcgaacccc gaaaaccatg gttcataatt accatttttt tctgattatt
    13981 ttccggttcc gacaaaacac aaaatatttt tttattattt atagtttcga aaaatacttt
    14041 ttcttcataa ttcataccct cattctactc tatttctgat agggaaaatc tgctcactga
    14101 cctccttatt atcatcgttt ttattttcaa aagagacgtt taaaaattta ttatttaaag
    14161 tgcctcggcc acagcgagga caaagatccc gtggggggaa tattttttcg ctgcaatgcg
    14221 gacactcctc accaaccatg cggtatcttt gtttttgtag tcgccagtat cttggtactt
    14281 ccatttattt ttctcgcaaa acaataattg tccagtatcc aagataaaga agtattaatc
    14341 cttgcgccca aattgctatt tgaaaaccct taccgcttat cattattaaa ctaccgtaac
    14401 gaatcaaatc ataaacaact ctcgcaataa tatataaaaa aagaggagac aaagccgtta
    14461 agctaatcaa aaatatttgt gatttatccc ggctttcttt taaactattt aaggttaaat
    14521 atggttctct catcagttcg taccagtttc tcccgagaaa atatccctgc ctaattccga
    14581 ttaggctgcc gagcttcagc aaattcttta ctcttttctt tatcatagct ccaaagactc
    14641 tggaatttct ttctggctct aatcagcctt gattcaactg ccttgacgct aattttcata
    14701 agtttagcaa tctgactact tttccagttg tctatatact tgagtcgtaa gattttttca
    14761 taatcttctc taagttcagt catggttttt ttaatttctt ctttgagttc attttttagg
    14821 acgtctcttt ctggggttat ggcatcctcg gcgatttctt caatgattgg attaaccgaa
    14881 aataagaccg tttttagtct tttctttcgg taataatcag taatcttatg tttcgcaatt
    14941 ccacaaataa aagaaaactc actacttttt ccattaaaag tgggtaaagc cttataagct
    15001 gccatcaaaa tatcttgagt taattcttca actacatctt catcgccgac tttttttcta
    15061 ataaaagcca ttaggctctt ttgatattta tttaccaaat cttccatttg tatcgatact
    15121 ttaccatatt ttaggttgga ttttattttt tagtttttag actttaggtt ttttctcttt
    15181 ttgctttata cttaattata tgttcttgcc caaaaactct gctcatcctc tctatccttt
    15241 gtcccgttct cttcgccttg atattttaga gatgattaac cacgccggat ttggccacat
    15301 tggcggttct ttatcttcaa ttgatatctt aatttctctc tataatagtg atttatttaa
    15361 ctttaacaaa gaccatttta ttctctccgc cggtcacatt tgtcccgctc tttatgccgt
    15421 attggctcaa aaaaatctca ttccccgctc ggagcttctt acttatggcc tttttgcttc
    15481 tcggcttcag ggtcatttaa atcttgagac tcctggtgtt gagtatgcct ctggggctct
    15541 tggtcagggt ctttcttttg cctcgggttt agctcttggc gataaaactc atcacactat
    15601 ttgtttgacc actgacggtg agcacaatga aggtcaaatt tgggaagcca tcgcctttgc
    15661 tagtaaatat catcaaggca atcttatcaa tattgttgac tcgaacggct atcagctggg
    15721 tggctctacc gccgagatga tgcccttaac cgagcttgcc tctaaatatc ttcactttga
    15781 ctggactgtc atcaccgtta acggtcataa tttttatgaa cttcaaaaag ccttaaaaac
    15841 tgccaaatca tcatcgattt atcccgcctg tattatcgcc aagaccactt ttgccaaagg
    15901 tgtctctttt atggaaaata aatttgaatt tcatgacgtt aaaaaattac ctgaagaaat
    15961 ttatttaaat gctaaaaaag aactggaggc caaataatat gaagactgta gattgtagat
    16021 ttaagatttc ttctatccgt caagcctttg ccgacactct ttttgaactt gctgttaaaa
    16081 accccaatat ttatgtggtt gatattggtc ttcgtccctc gctttatctt gatagattcg
    16141 ctgctcgttt taagagtcgt tttatcgaat gtgggatcgc cgaatcaaac gctgccgcgg
    16201 ttgccgctgg tctggccgcc tccagtaaaa ctgtattctt aacttctttt gcttgttttt
    16261 ctcctgccct aaactgggca gtaattaaac agtctatttg ctacaaccac caaaatgtca
    16321 aaattgtcgg ttcacatgct ggtctcatga gtggtgacct tggagccact catcagatgc
    16381 tcgaagacat cgctctcatg cgctctctac ccaccatgga agtttttgct cctctagatg
    16441 ccgtcgagat gaaaaaaatg ctcccagttc ttgccagtag tcctcaccca gcctatcttc
    16501 gactagttcg cccccctacc actgaagttt atccaccaaa attatccttt actatcggta
    16561 aatctcaatt aattcaatcc ggtcgtaata ttactgttct tggttatggt cccatcttaa
    16621 ctcagctatt ttctgatgat tttaaaaaat attcattgga catcattaac tgttcctcga
    16681 ttaaacctct agactcagac actattctaa aatcaattaa aaaaactggt cgttgtcttg
    16741 tcattgaaga tcatcaaaaa aatggtggtc ttggtgatgc catcgcaaat ttaattttat
    16801 cttcggggat aaaatctaaa tttattcacc tggctgtcga cgattctttt ggtcaatcag
    16861 ctgtaaacta ttttgattta tatcaccact acggcctttc tcccttagct attattgaat
    16921 cgttaaaaaa gttattatct aaatcatgat tcctttaaaa aatttactct cagactatca
    16981 acaaaaacat caagccctgc ccgcctttaa catcgattct tttgagattt atcaggcagt
    17041 agaaatggcc gtttcagagc taaaccttcc ctgtcttgtt cagctttctc cctccgaaga
    17101 taaatttatt ggggctgaaa ggcttttgat gctcgttaaa aaagctaatt ctgaaggtct
    17161 cccgatatat ttaaatatcg atcatggtta cgacgttcgc cgtcttgaac actgtttaaa
    17221 aattggtttt gatatggttc attttgatgg ttcaaaaatg gactttgaca ccaatctcgc
    17281 taccaccaaa tattttgtta gccaaatgaa agaaaagtac cccggtgccc taattgaggt
    17341 tgaatttaat catatcaatc tagtgggtgc gaccccctca tccgattcct ttaccagtcc
    17401 ctcggaggcc aagcgtttta tggccgaaac tggggctgat cttttcgcct tatctattgg
    17461 taatttacac ggtgtaaata atgatcttcc cgaacaccta aatatcagcc ttctctctga
    17521 aattaaacaa actttgggaa ataccttctt gactcttcat ggtggctccg gagtccctct
    17581 tgaccaaatc caaagtgcta ttagtttagg ggtagtcaaa attaatatca ataccagcct
    17641 acgtctaaaa tttattgact cacttcgtca tcagcttact cttgggcatg gtgaaaaatt
    17701 atatgaatac cttactcctg tcattaacga tgttaaagag atagtgaagc aaaatctgat
    17761 aagtttttct cattgacaaa acattgtcaa aacaatataa taacaatgta tgagtcaaac
    17821 cattaatctc caaattccca tcaaaaccga tctcagggat cgggctctag caggtgctaa
    17881 atcacttggt tttacgaatc ttcaggattc tatacgtatc tttttacaac agctggctct
    17941 gaataatttt gatatttctt ttcaatctaa accagttgtt ttatctgtca aaaatgatcg
    18001 ccgttattcc aagatgattg acgatcttaa tcgtggacga gaaaaaacaa ttgtatttaa
    18061 taatactaaa gatatgttta aatatcttga tgatccaaat agaacttcat cgtaacttta
    18121 ttaaatcata cgataaacgt attaagtcaa attcaaaatt actttctcaa gtaaaaaaaa
    18181 ggattgaatt atttcaagtc aagcccgagg attatcttct aaaagatcat ccattaattg
    18241 gccagaaaaa aaatctgaga gccttttctg tgagcggtga tattaggata atttattatt
    18301 atttatcagc aaataaagta atcttttttg atattggttc acataatcaa gtatattgat
    18361 ttacaattaa attatgtttg acatcatctc cattggtgct gccaccactg atatttttat
    18421 taaatcagcc gagcttcttg aaaataagcg ttttttatca ttaaaaagat ccagtaaaaa
    18481 cgaaatttca gaatccctaa tctgtagtgg tgggggagcc accaattctg ccaccacttt
    18541 ttctcgcctc ggattaaaaa catctctaat ttcattaatc ggagatgatc ctttatcgga
    18601 gtacattcag actgatctca tcaatgaaaa aattaatcgc cagtttctaa ttgtcaacca
    18661 aaaagaccgc agcgattttt cccttatttt agtttcttca aatggcactc gttctatttt
    18721 aacgagtcga ggttcgaccc ggcttgaaag taaaaatata ccctgggata gactgaaaac
    18781 caaatggctt tatcttacct cccttgaagg caatctgccc ttacttgagg aattaattgg
    18841 tttttccaaa gagaataata tcaaaatttc ctttaatcct ggtcttcggg aactaaagca
    18901 gcgtcgtcag cttatccctc ttttgaaaca cctcgacttc ctcttactta accgtccgga
    18961 agctgaaatt ttaactaata ccagtcataa gaactcatct ttttatgaca aattaaaatt
    19021 atttnnagtg agaccctatt ttctccaatt attaataccc acgccgtcga cgaaactgga
    19081 gccggtgatg cctttggttc tgcatttgtg gccggcttga tgcaaaatta ttctctggaa
    19141 caatgccttt cctgggggat tcataattcc gcctcggttg tttccgccct tggnnatgtc
    19201 tctaaaatca aataaaaaac ttaaacaatt tccttcagaa gaagggaagc agcttgacct
    19261 tgtcgaaact atcaatgacg ttgataaatt aagaaaaaaa cggaccacca ttattctttt
    19321 tttactgctc acagttggtc ttagtttttc tttttggttt tatcgggagt ttcaatcttt
    19381 tgatataaaa aaatttaaaa tgcccagcct ttctttttcg ctttcaaaat tttcacctga
    19441 aatacctgaa aattggtcag tttttgttag gtctatcggg gttaccgatt tttcctattc
    19501 ttcaaaattt aattcttccc ctgattttag ttatcttact actcctcacg agccttccta
    19561 cgccaaaaaa tatttacctg agggggtaaa agttattgaa aaaataaaag acaattctga
    19621 gtcttttgaa atattatcta aaatttctac ccccaaaaat caatttgaaa tatatgccaa
    19681 aattcccgga aaaattaatt cagattctcc tgaaattgag gttttttcaa atctcgttac
    19741 gatcttttat tggcatttac ttaaatagac aagactgggg ttggagtcgg tgaaatacat
    19801 tttattttct tttcattacc atcatgaaac cattcatttt taccacaagt ttcaacttta
    19861 tataaacctt ttggcgtttc ccagttttca ttttttttct cgtcaattaa aaatctcatt
    19921 gttcgattcc aaattggagt cgctccagaa attccggagg ctacccagct catcggcgag
    19981 ttgtcattat tgccaaccca gaccgctacc agatatgttg gcgtccagcc aatacaccag
    20041 ttatctttca gattattggt agttccagtt ttcaccgcca cggttttacc ttttatttgt
    20101 aggagagaat tagtcccgaa gataggcgct ctggcctgat tatcactcag ggcactatta
    20161 atcaaaaaag tatcgatttc cgaagcaatt ttttccgctt ttgtttcttt tttataaacc
    20221 atctctccaa gataattgtc aacttgcaaa attggattta cctcgacttt atttcccaaa
    20281 ttcgcaaaga cactataagc tcctgccatc tcgattattt ttacttctcc tgccccgagg
    20341 gccaaagata gaccaaatcg agatctgtcg gtccaggtgt taattcccat atcctgagcc
    20401 aaatcaatca tattatcaac cccgttttca ttaagcagtt ttactgaggg aacattaaga
    20461 gaggaggcta gggctgttct tagggttact gttcccaaat attttccagt atagttttga
    20521 ggggtataat ctttttgacc agctatttta tatcttactg gtttatcttc aatcgtatcc
    20581 cagaggtttt ttcctttttt taatgctagt aaataattga tcggttttat ggacgagcct
    20641 ggctgtcgaa gggcctgggt aaccacatca aattttccgt caatatcagt tgcaaaatag
    20701 tctttcgagc caactaaggc aagtatttca cctgttttta cgtctaaaat gacactagct
    20761 ccattgctaa tttttaatcg actcgctttg tccacttctt ctttgacaat tttttccgct
    20821 tccttttgaa tatcataatc taaactagtt attactcgca ggccctgtct acccacttcc
    20881 gtaaaaccca atttttctaa atagtttttg acataaaaaa caaaatgagg cgccaaaata
    20941 tttctttttt ccccaataat ttttatttcc atctttttga ccgtctcagc tttttcaaaa
    21001 tcgagcattt tagcattcac catttcattt aaaacatgtt cttgtcttga ttttgccagt
    21061 ttaaatccct cttcattaaa aggggagtat gaaccagggg cggctggcaa tccagccaaa
    21121 aaagtggcct ctgccaaatt taaatcttcg acttttttcc caaagtagcg accagccgct
    21181 tctgccactc catatatgtt tcccccgtag ggaacttgat taaaatatct ttccaaaatt
    21241 tcatttttac tcaacttatt ttcaagcatt attgccagta tggcttcttt tatctttctt
    21301 tcccagcttt tttctccatt aagaaataca ttttttacca gttgttgact gatagttgat
    21361 cctcctctca acttttcttc gccggtattt ttaaagttaa aaatcattgc tcttataatt
    21421 cccctgatgc tgagaccatg atgctcgtaa aaatctttat cttctattgc cagcgtcgcc
    21481 gaaattaaat ctgggggaat ttgagacaaa tttacccagc ttcgatcttc atcttcatga
    21541 aattggtata aaagtagtcc ttgtcggtca taaatctttg tcgacaattt tggtggatta
    21601 tatatttcat taatattcgg taaatcgcta aaaattcttt ggtaaatcca caatagtaaa
    21661 ccagttatga gcaaggtcag caaaaataaa gccggatatt tccaatttaa aaatactttt
    21721 tttttattaa ttctgattaa tttatttttt ttgaggagaa tattttttct ttttttcctt
    21781 ttaataaaaa tatcttttgg cagttttatt ttcgacaggc ttttaattat agttaaaaat
    21841 ggtttaccaa tataaataaa aaaattaatt attctcttca tctttcggac aatctaaaca
    21901 aacccattct cctgtcgcct cttgaacact gagtctattt tgccactccg cattggggtt
    21961 attttcaccc tcttttacaa ttttaccggt tgttttatca atcaagatgt tgttttgtaa
    22021 agggattctt aattttggga caaattcctt tttaaaaagt tcgttgtgtg aatcgcaacc
    22081 tccctcgggt ggcaattgtc cggttaaatt acagacattt attgtaatta ccccactagg
    22141 tcgcgtaaaa ttttttacgg ctttattttc cagcacctta ctcattattc gattccaaat
    22201 tggggcagcg ccagtagttc ccgagacgag ataacccatt tttgaattat cattatttcc
    22261 gacccagact gccaccacat attctggggt aaatcctatc gtccagttgt ctctcatctc
    22321 attggttgtt cccgtcttta ctgctacttc agggtggccc ttaatattta acattgagcc
    22381 cctcccaaaa accatacttc gcgccccatc atcggataaa atatcagcca caataaaagc
    22441 cgtttctgga gataagactc tatcgcccgg aaaataatta tattcttcta aaatttcccc
    22501 atttttatcc accaccttca aaattgaaac taaattttgc cttattccca tgtttgaaaa
    22561 aacaccaaaa gccacggcca tatcagtcat atagacctca ccgcctccca gtgttagtga
    22621 tagtccataa tcactagaat ttttaaaagt actaattccc atagctgagg ccgatgctac
    22681 aaatgtttga accgtgttta agctcagcat ttttaccgcg gggatattta aagaattacc
    22741 gagtgaattt cttaaagttt gaagtccaaa ataccgataa ccgtagtttg ttggacaata
    22801 agctttttga ttctcaactt taaaacaagt ttcttcgtca ttaaaaactg atgccgctgt
    22861 cacctttccg ctttctattc ccagggcata attcagtggc tttattgatg aacctggctg
    22921 tcgaagtgcc gttgtcacat tatatttgcc atcaatttca ctatcaaaat aatcttttga
    22981 tccaaccatg gccaatattt gaccactttt gctctcggtt attagagccg ctccattgct
    23041 caccttttgt tttttaagtt tttcaatttc ctttgccacc tcttcctcgg cgtatttttg
    23101 taaatctggg tcaagagtgg tccagacctt taagcctccc tcagttacct ttttaattcc
    23161 atatttctct attaattgtt ctctgacata aaaaacaaaa tgaggagcat taatctcatt
    23221 tctgttaagc ttataattca attcttcttt tattgccttc tcatattctt ctttactaat
    23281 atatttcagt tcttccattc tttttagtac tagttcttgt ctgtttttgg ctgcgtctgg
    23341 atgagcaaaa ggggagtatt ttgttggcga tccgggtagg ccggcaatta aagctgcttc
    23401 tgccaggctc aagtctttga catttttatc aaaaattccc cttgcggctg actctactcc
    23461 ccacataatt cctccgtacg gagtttgatt aaaatacatc tccaaaattt catccttact
    23521 atacataatt tccgtaatta ttgtcagcag cgcctcttta atttttcttt cccaggttct
    23581 ttccggactg agtaaggcgt tttttactag ctgttgggtt atggtcgaac ccccctgaag
    23641 tcttttttga aagatcgttc gatataaacc tctaattatt cccttgatat caaaaccagc
    23701 gtgtttataa aaattattat cttcaattgc tagagttgcc ttaatcattt tgtccggaat
    23761 atcggcaagg gttacactgg ttctattttt atccgcataa atctcataaa gcatttttcc
    23821 gtttctatcc attatctgtg acgattcggc atactctcca gatcctaact ttttggggtt
    23881 gggtatatct ttgaaaagca agtaaagtaa tataccagca gccactatta ccagtgacca
    23941 gatcagcctt tttctcttaa ttttgttttt tttcaaactc atttatttct tgcaaaagcc
    24001 ttaaatattt ttgctcatcg attttatcat ttttacccac ttcttcaatc atctttctgt
    24061 atacttctgc catctttttt atttgattat ttatctcatt tttatagata ggcttatttt
    24121 caaatttatt aatcagattt ttcgcctctg ttaatttttc tatagcttta tggtagctgt
    24181 ctagggctaa actatatttt tctttttcca tcaggtttct ggcttcagag acttttttat
    24241 ctgccaatat taaagttatt tgaattatct tttcttcatc aaaactaaat ttttgccaaa
    24301 accagtcccg aatttttttt atccagtaat aaccgctatt tggtaacact cggctttggg
    24361 gtagtttata tatttcta
//
LOCUS       DHGB01000053           11447 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_93734, whole genome shotgun sequence.
ACCESSION   DHGB01000053 DHGB01000000
VERSION     DHGB01000053.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11447)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_93734"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    1576..1686
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3361 a         2080 c         1800 g         4095 t
ORIGIN      
        1 tcaaaatcga agttagggct ccggtcgcct cggggtgcaa tctttcttct cggcgcagat
       61 gttcttgaat attgaccggt aataaatctt tattactata aattggcgct tcaaccgaca
      121 tgtgaagcta aaataccact ttaaacaggt taagtcaacc tatttatgaa aaattacaaa
      181 aaaattaaga ttcgctcggg aaggcaactt gatcgttaag tggttttcct ttgttgtatt
      241 cacgactggc aaatgccagt gctaaagtgg aaatgatttg ttctttggag actgttatgg
      301 taaaaagcat ccccgcatct aagggggtaa aatctatttt ctccggatga ttatgaatat
      361 atgggtcttt ttcgttcttt ttaaaaaatc ctactttaac agatccatct tcattttcta
      421 tatagccaag agccacaact tctatatatt tgtaatctga agtactatag cctttagttt
      481 cttcattgta gggttttgta cccgacatta attttctttc caaaactaaa aaagtttcat
      541 cagaacttgg gctgagaata taaggactgg tatatttttc aggatcagct tcatttttca
      601 aatcaactat cggcaataag gttttaagaa acttttttgg attaccatca ataaaatctc
      661 tgtccctttg aagccccctt gctctttttt cgatctttat taatttattt tctatattag
      721 atttgtatat ttctttcgac atgattaaag tatattctaa aaatattaaa aaatcaatat
      781 tttacccata aacaggcaaa gaaagaaaat agtttattat atttttcaat caaatcccga
      841 tactttgtac cggttgtata cctttgagac tctgggtatc ttttgagtaa ataatctggt
      901 tgatgagaat ttttgtagaa atcccttatt tacccaccaa tgaagggttt tagttcctcg
      961 ctaaaagtcg actcatctcg gtagccaata aaatccttga ccagttctcc cttgatgaac
     1021 aatttcatgt tgggaatact ttgaatttgg taatccatgg ctagctgttg gttttctggc
     1081 aggtcagtat caaccttggc tacttttaat ttaccctcaa atttagtggc cagttcggtc
     1141 aaaattgggg ccatcatttg acaggggcca caccagggtg cccaaaaatc gacgagcact
     1201 ggcagctcag atttaataac ttcattcaca aaattttcct gaccgttgac attaattgga
     1261 ctggacatat tttttaattt taattaataa ttttttatct tcatttttaa ctgattttag
     1321 acagctttca aattgctcct caaccaccgt atccatgaca ctgccaatag ctgccttggt
     1381 ggccttaagc tggatcaaaa ttttaagaca atcctgattt ctatccacca tttttttaat
     1441 cccttcaact tgtccggcaa tattatttag cctttgttga attgatttca taatataatc
     1501 atatcctaca ccccccaggg gtgtcaataa ttaatttatt aagtcaaaag tctctgatca
     1561 ggtcaaagtt attgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnncctt gacccatgcc atatttgctt ctaaaagcat taattaagct attatcacct
     1741 ttttgtgaag ctacttgagc actggcgaat tttttaaaat tagattcatt gacgaatctg
     1801 gtagcacctt ttccggccat attagcggcc caggctttat aatcaccttt ttggaaagcg
     1861 gtaacattaa tattgtgtct ctcctcggtg taattaggac ctttgacggt cgcatcacca
     1921 cgataagcga gaacaggatt ggcagattta acagcgacta gcccaagggc taaagcggct
     1981 aaacctaaaa cgattttggt attcatttta tttttttaaa ttataaaaag atgaacttga
     2041 ctagtatgtg gtccatctag tcttagtaca aggcaagttg actttttctt tcaatttgag
     2101 aggcggccag accagggttt gatatcatta gcgacaaaaa tgtcaccctg aagcaatccg
     2161 gtgattttga tttcccgacc aagactgata tcaatggagg gacgaatgat ggctttttga
     2221 atatcaatag tccactgatt cccgctaaag tcagttaatt caaaagaatt aggagaaata
     2281 atttgggtaa tagtcccaga gagacggccg gcactagaat tagtccaaac ctgagttttc
     2341 attggtgcca cccgacgata gagaggtaaa tcctttgcca gcagagaatc aaatttttga
     2401 ccaagattaa aaagagaaag tagggagcca agggctaggg acagaaaaag ggcaaagaga
     2461 ataatttttg gtaattccaa acggtaagct tttttagttt tggaaaaaat aaaataggca
     2521 aaggcaataa aaagaaggag agaaagaagc caaaaatagg ggaaataata gagatcccag
     2581 tcattgcttt tgaacatgta aattagagtc gaaaaggcca ggccaccgat aaagatagaa
     2641 agaagatata gaagccagag taaatagttt ttaaccaaaa attcccacct gggaatggga
     2701 cgaagatggc gtttttgaat attttggaga acctgatcac tcagagattt tttatttttt
     2761 ttcatatatt tttttaaatt gtattttggc gcgagagatg agagtagcga cggtacccat
     2821 gggcttttgt aaaatgtcgc taatttcctg ataatccttg tcttcaaaaa attttagaac
     2881 caaaacatct ttgtactccg gctttaattg attcaaaatc ttttttattt cttggccctg
     2941 atggagggcg atggtttctt tttcaatatc ggtatcagct accagccaat tgacaaaatt
     3001 ttcattatct tcaggcaggg aaacctgaaa ggaggcccga ctttttttaa aaaaattaat
     3061 agcttcgttg tggacgatgc gatacagcca ggtactgaat tttaaatcac aatcacagtc
     3121 gtttagatgc tcgtaagctt tgataaagga attctccaca atatcatcaa catcatcagg
     3181 tgagaggaag agaaaacgat ggacgtaggc agagagtttg tcttcatagc gtttcatcag
     3241 acaataaaaa tattgactgt cctcaaggga gagggcgacg agctgcttat catctaattt
     3301 ttggcattcc cggtctatca tggtttatat acagtatata tacaattgga gtgggggttt
     3361 tctttcaaaa aagattgatt ttaattgtgg caaattcgcc ataattaaaa tcaatcaaat
     3421 tatcctttta atttttttgt tattctagat tccaaataat tttttccagt gattactttt
     3481 ttcccagttt tactctcaat ttcctgacga gcatggccgg cgacactacc accttctctg
     3541 gccactacct tattttggcc aaaagtttcc ggcttttttg tttttgatat ttcagtggta
     3601 gctgtttcag cgagcatgtt tagcaccagt tccatgttgg tcatattgtc acgcaaattt
     3661 tcttttttaa gattttttaa acttttatat tctttggtcg tcatccctgt ccaggccttg
     3721 gttatttcat tggtcaaaat agcaaattcg aaaccatcat tgacacctct attttcccat
     3781 tcatcggtta gatcttttct aatttcaata cttttaagac ggagatttat ccactgccga
     3841 ctgtaacctt tttgatagta ggtttttagg gctcgattaa tcgcaatttc tggatcagct
     3901 gtttcctcga gtctttcata gccgactctg gccagccaca atttaaatgg ttcggcgttt
     3961 ggtgagggaa ttgactgtat caaacgtaat aatgtttctg tatccgccat gtcagtgtct
     4021 cgttttttcc catcttcagc cactatcttc aaccgtacga ttttttcgta cacttcactc
     4081 ccctcatttt tcagactatt ttttaaatct gaccaatatc ttttaggaat cgagctgttg
     4141 gttagtgttt taataacatc gattatcgaa aaataccaaa gttccttatc tgcgtcccat
     4201 aggcggcgga tttgattatt tccaaaaatg gcgactgttt ttttgtttag tttcatacta
     4261 tacaaataat aataaccccc cgacttcgct aaagctacga cgggcaagga agttaactag
     4321 ttatattttt tggcctcttc ccagtattta caatatttac caaggatgag ataatgattt
     4381 taagaaaacc ggtaagtgtg accaacgtcc gctagccggt ttttatagtt tttaaggcat
     4441 ctcaaccagg ggatttattg ctttataaag ttttgcttta tctttttttg tagctctccc
     4501 tacaaataat ttattttcaa aactattaat taatactttt tggattggat ttaattgttc
     4561 aattgttttt attttatttt ttagttcttc tttaattttt gggcagatac catatagatc
     4621 agatatttgt tcttctgaat cattgtttag ttttgtgacg aagctaattg aagttaagtg
     4681 ttttttagaa tcttgagggg aaatgcataa gttactaatc ccattgacta aataatgaaa
     4741 aaaattttga taaataataa tatcctgttc agaattgctg gcttcactaa caacttgacc
     4801 tttaagattt ttagcaacca aaaattttaa taaatttgaa aataatagac gatatgatct
     4861 gtttaaaacc gttattttgg cccaaccaag tctaaaggct ttttctttgt taacgatggt
     4921 gctcaatata aaaaaattgt gttttttaag aaatttattc aaatctatcg aaaaattttt
     4981 aattttttta tctgttttta aaaatcttcg aatttcagtc ccatgaataa catacttttt
     5041 tgaaccaaaa tgtttaagtt ttaaatcttc aaaatcaatt tttaattttt cccgattatt
     5101 ttcgccaatt actaaaccaa tgagagtaaa atatggttga gtttgatacg attttacatc
     5161 ggaggttcct gattcatcaa taaaaatata atgtttttta tcaacactag aaatcatgac
     5221 ttagtctaac actatttgtt ttaaatatta cattaatcaa acttttttgc ctcttcccga
     5281 tatcgacaat attcacactc agtattcgcc tcgggtatcg tgtcctcatt taaacatttc
     5341 cagatctcat ttatcgttcc ctccacccaa tcaggattgc cggtgtatgg gattaattta
     5401 atatcaaatt ccaatttacc atcaaaagat tcctgcgccg ttcgcccatt gcagtacaca
     5461 aaataacccg tctcggccac tttaaaatca ttttttctaa ataaccactg ataaatttcc
     5521 atttgtcgtt tatagccaat ttgccaatcg gcatcgagat ttacttcact atttttggcc
     5581 gtcgctttat aatcgacgat aattagttca cccctcggat taacccacag atcgtcaact
     5641 ccaccggtga taattaaatc agtgccggga aaagggtagg tgattccccg acgtagtgaa
     5701 tcccgccatt catccatttt tggatgcgaa aaggggatgg cgtcaatgcc ataggctttc
     5761 attaaggggt ggggagtgtg cccggcccgg tgaatatcaa attcgttttt gagtagccga
     5821 tcaatttccg aattaatcgt aaacggatac cccggcggtc tttttacctt taatttcacg
     5881 tccaaataaa aacatttcgg acaatttagg tataaatcaa ttttgcttcg tgataatcgc
     5941 atagatttat tcatattaat tattataata ttttttttat tatgccttct tttaccattc
     6001 ttctgtaaag aacatcccct gaaaccttga aaagagttat taaatcaaaa attgttgatt
     6061 ggagtagctc tacatcttca ttattcggta tttttccaag tcttttgatt aattctgcgt
     6121 ttagtgattt agtggggacc aatattaaac cagcaaaagt ttgtgcctga atttccatat
     6181 aacggtggtc tttatcgtta attttcgaga agaagtcgtg atatttatta aaatcggtcg
     6241 gaccattttt ttggtaccaa ttttgatgaa gaattttgtg accgatttcg tgagctatag
     6301 taaatcgtgt tctctcggga tattttatgt aacaatgttc gtcaatcgtt atttgagaaa
     6361 aatctgaggt gataaaaccg tcaattccga ttaggttttt tatccctgga atcacacata
     6421 aaaagatttt tagttttagt tcagcaattt cctcaatagc caccggtaac tgattattag
     6481 aattatattg atttaagaat ttgtcagccg cacgccgtac ctccaccatt gaaagagtca
     6541 ctccatccat gttaatttgt tcccttttcc ccgtcgttta gtaaagctat caattcttta
     6601 actttctctt tgtttaattt ttcaccgcgc attgttctat aaaatgcagg gagaactgac
     6661 atcatctgtt gattatttct gatatcaaca gggattgttc ctttggctag atgagctaag
     6721 tcaaaaaaag taacccattc cagagaacct tcgattattt gaagcgcttt tgcatatcct
     6781 tttaaaatac tttcatcgat actcggagtt aatatatttc tttccagtcg actgatattt
     6841 ccaggatcat atccaaatct ttcacagaat gatcttaaag taaatcctag ggatacccta
     6901 cgatttctaa aaaaaatgcc aaagttttga ttggtttgat tttgtctatt gttcataagt
     6961 gttgtacaat tattacaaca cttgatctta ttagtgtcaa gcacttaatt attaaacaga
     7021 aatcccggcg gtcttttaac ctttaatttc acgtccaaat aaaaatactc cagataattt
     7081 aagaataaat caattttcga tctcgacaac ttcatcttaa tgagttttta aaaatttttc
     7141 gtgtacttgt ttagccaaaa atacggcata gtcaatatcc tcttctcttt ctaccataaa
     7201 tgaactttgg tgtacgctat agtgcttata actgtcttta acatatttta cttttttctc
     7261 cggatctttt aagtcaaaag gttgtactct catcaaaatc atatctaacc ccgacttgtg
     7321 aacatgtatc gcaataacat ttaatctttc tagtttaaac ccaataaaaa attctttgga
     7381 tcctcaatta agttactatc cagagaaagt agtttaattc ttaaatcttc ataaagttcc
     7441 ttactgttat cccatccttt tttaaaatgt tcttcgacgg tatatgtttt tacctggctt
     7501 ataacctcgg aggcagaatt gtttttcaca accttggtaa tagattctgc ttttttacta
     7561 gattaaattt gtcggacatc caacagtcca tcttcatatc gattaaattc ccataattcg
     7621 atcggaaaat ctctgaaatt tgtcgctccg tgctggtact gggtaaatga tttagcaacg
     7681 aaaataacgc gcacttgaga ccagtcaaag tcagtaacgg tagctttttt acctgttttt
     7741 gtaatataag ccaagacaaa atttgcctta ttattcagca ttaatgacag ataatgtaat
     7801 ccctgatcca cgacactaaa attactattc tttttgtatt ctagaatcac aaaagaatta
     7861 gtttccggat cataagctag agaatcaaga aaataacctt caatttgaaa ttcgccatca
     7921 attaattgaa gcccaaacag ttcatctaaa ttcgcttcaa tcagatcgtg catttctttt
     7981 tccttagaaa attctttctc ctttatagat gaaatctttt gattttggat ttggtagata
     8041 gtcatctttt atttaaattt taactttcaa tgctttgtat tttttggatt ttcataattt
     8101 tttattctat tatttgccag atcgatataa tttttttctt tttcaattcc tatccagttt
     8161 ctaccaagag ctttcgcaac aacggcagtt gttccactac ccataaacgg atcaaaaact
     8221 aaatcgcctt tattgctcgc tgttagtaaa atttgttgga taagttttaa tggcttttgt
     8281 gttgggtgtg cttttatgcc gtttttatct ttcaaccttt ctttcccttt gcaaagtccg
     8341 aatacccaat cagtatcttt catttgaata cctccattta ttttctttaa aaattcataa
     8401 ttgaaagtat attttttgca atccttattt ttcaccgacc aaattattgt ttcgtgatta
     8461 tttgtaaaac gacgaccatt caaattcggc aatgcgtcgg tttttaccca tatcacatca
     8521 ttcaaaaacc aaaatcctaa attttgcatt attgtcccaa ctcgaaaaat attgtgatac
     8581 attccaatga cccaaaatgt acctgtcggt tttaatattc tttgacactc ttttatccaa
     8641 ttttgagtaa aattgtcata atcgtcatag ccttcaaatt gatcccagtc actatcaaca
     8701 ggtataacct ctgaaccatc agctcttttt aatctttttc cttttggaag ttgtaagtag
     8761 taagggggat cagcaaaaat taaatcaact gaattgctag gaaatttctt catttcctct
     8821 atgcaatcac cgtgtaaaat tgtgttaata aatttttgca tacgacatca aaaattataa
     8881 atgacaaggt gttcggcatt tctgtcgttt ctacccttaa cattatagag ataagttttt
     8941 tcaaaactat ctactttaat accttttttg tttttatata tatcatatat aaaaggagtc
     9001 tttttgatta ttaatataaa atgagctttg tttgtataaa gacattttgc caatctttct
     9061 tgatcttttt tatcaaaact ctttttttca taatcgttga aatccgtatc atacggtggg
     9121 tctaaaaaga taaaatcatt tttgttcaaa tcttttcgag ttaaaatttt ttcaaaatca
     9181 gtgttcaaaa tttctgcatt tttaaatgct ttctccacct catcactgaa tatataattc
     9241 accttacttc taaagtcttt attgttatat gcaattcctc cataaggaat attgaaatca
     9301 cctttagcgt taaatctaaa catcgaggca taacaaaatt ctctaataaa atagtaatta
     9361 gcaatttttt tagctaaaga agttttatat tttgaaccat ttttattcat cacatctcta
     9421 aagtgcatat aaaatccact tctaaaagca gtttcgatat ttttatgtaa atctccgtca
     9481 ggtaatgccc ctcgttcctt ttcaaccttt tttgtcctgc ttattttaga aattaaattc
     9541 tttattattt cttgaagcaa attattcgga tcgagggcaa atttttctaa gaacaagcca
     9601 ttaaactgat cttctttggc ctttaatttt tcagttatta tttctcttac ttctttctca
     9661 gctattttgt tattcttatg gtcatcatag agcttaacga tttcattttc gaatatgttt
     9721 atatactttg ggattttttc ccaattatca acataatcat acaaacaact cttaaattca
     9781 gttttatttt tttcgccttt aacaaaacga taaaattcta ttaactcttc gcaaatgtca
     9841 ttgattatcg ctttttgagg ttgaagttta aaaaatattg caccaccacc aaaaaatggc
     9901 tctatataac gggtgtgttt tggtatgaga ttttttattt gctcatattc tttagatttt
     9961 ccgcctggcc atttaatgag tggttgcata tttatcctaa ataagtcatt ttaattgaat
    10021 ctaatgagct tttaataagt ccagcctttt ctttgtaaaa ttcaagaaca atatcgtatc
    10081 cctcacctga tttacagaca aaatcccaga aatcttttcc tatcaaaagt tcatcatcgg
    10141 aaaaatattg cctaactctt tcctgtttcc agggtttgcc ttcaccaaat cgattatagg
    10201 cggttgcaaa aaatacctta atattcgttt ctcttggtaa ggtatttgat aaaatcgtga
    10261 attgctctaa caatgcctct ttttctgatc gagctttttt attatccaaa tcacctccaa
    10321 ttttcaattc aattaaataa ttatcacccg tatctttatc aatcaagtaa taatcactat
    10381 catgtctttt tgtaacgagc gattgttctt caggtttgac tcgcaaaaat tgataatcgt
    10441 ccacagaagg tggtgttatt tctcttcgtt tatatttctc taataattca gccatatcct
    10501 gagtttgttt taaacttaaa ggcccttcaa cattttgtct aacttcataa gataatttag
    10561 cgatgtttat ggccattttt tctagcatat ttcccaaaga gctatcaaga gaacgtacta
    10621 atgcagtgta atattgaatt tctggtccaa gaacagcgat aaaaacattg tgaattttca
    10681 tattcagtgt accccctgga tcttctaatt ctccttcatg tcttccttga aatccgacag
    10741 caaacgattc aacggatgca taaacgattg atcttatggc ttctttttta tctaatgtat
    10801 tttgtgtcat aaagtttttc taaattattt tgataaatta tcaacccaat tatttaataa
    10861 atctattggt tttattctat ccaaaatttt tatttgcatt tatatttcat tataccgccc
    10921 tcgggaggga aaactgggag ttgaagagaa attcttatag aaattattgt taatacacct
    10981 tgtcgtagca gttggcgctt ttggtgaagc cggctaagag ggcatcggat tcggaacaaa
    11041 accactggtc accttgatcc agctcgaggg agacattgct gtaattgcca cagccgggga
    11101 agaaataggt ttttttgcct tcacgggcat tgcccttgat attacatttt ggattttggg
    11161 gattggtgac ttgaaggcac tgatcactaa aaacgccgac tttttcggtt ttgacttgtt
    11221 gaaaagcagc ttttagattt tcggactggg aggaggcagt ggaggtgaag cgggcccagc
    11281 cggagagaat catttcttta tttaccaact gatcgtcaac ataaactagg gctaaaaagc
    11341 ggccaaagtg atcgtttaga tctccttcta gcctgacttt tttaccatca atgagactct
    11401 tgagtttgtc ggtggcctcc acgctcccac aaagaccgac ttcgggg
//
LOCUS       DHGB01000054           10236 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_44244, whole genome shotgun sequence.
ACCESSION   DHGB01000054 DHGB01000000
VERSION     DHGB01000054.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10236)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_44244"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         2744 a         2504 c         1482 g         3506 t
ORIGIN      
        1 tgtgggcaat gatagactcg aactatcgac ccccgtctta tcaggacggt gctctaacca
       61 actgagctaa ttgcccttat tttccaatta gtatatcaca ggaattttag cttaaaatca
      121 gacaattgaa aactttcttc tgtgattttc tctccatccc agacctctat cgccggtgcc
      181 ttatcggccg aaatcgttgt ttctcctttt ttcactcctt ttatttttaa aatacccaaa
      241 actacctcct cgttaaccag catctccttc tctccctcac atctcccttt tattaatatt
      301 tttcccaaat tttcgtcaat tgtagcctct actccactat taaaaatctc tttgttgacc
      361 tccacactta tgatttttat ttcacccggt ttataattta aattgagcga aaaactgact
      421 atctttcctt tttcatgctt tgccttaatt tttatttcct tttcagtctc tactaaaata
      481 tcatccggca cctcaaataa tatctgtttc tcagttaaag ttaaaattcc tccattttta
      541 ggcacaaacc tattgtttac ccagtaatat accgccattg ttaagagcaa tagtccaaga
      601 gccagtagta cttgtttcat ggttctattt tagcagtaaa ctcagttttt atggactatc
      661 atttcaacaa tttttttcgc cacatttatc cccgttgcct tttcaaaacc ctgaaactga
      721 cattgtctgt ttacctctaa aatataatta tcataatctt tttctatata tttttcagaa
      781 tttagatttt tggatttagg atttttcata atatctaccc ccccatagtc aagaccacac
      841 acttttgcca ctttttccgc caaatctctc tcttcttcgc tcaagttcca cctttccacc
      901 tcacccccca gtgaaaaatt actcctaaat tcctcgccca ccgccgatct tttcatcccc
      961 ccaattgctt ttccatccac aatgaccact cttatatccc attttgtcgg taaatatttt
     1021 tgccaaagaa acattcccag gtttttttca ttaattttat taacaaaatc ttccatttct
     1081 tttttatttt caaactttct tactgatttt ccttgataac ccctttcatg tttggcaata
     1141 actggatact cccaggcttc caataaaatt tgctcccgag tataaaaaat ttttgtcggc
     1201 actatcggaa tattattttc taaaaatacc ccatgctgag atattttccc cattctcgtc
     1261 catcccaaat atcccccagc atttacacaa aaaattcctc tttctcgcaa gattcttatt
     1321 gccaaatttc tcgcctctgt atattttcca ctttttgtcc ccgccaccct aaaccataat
     1381 cctaaagtat ttcccaccat caattcctct cctcttacca ttactctaga ttccacatct
     1441 ttaaggtcaa aacttaattc ccgatacagt gcccgattaa cctcaattcc cttcttcctc
     1501 gcctccgcca ccaacctatc cacttcaaac tggctttgtc tcggcgacat cgtaaaaact
     1561 acaatttttc tgttcataat tttatttata aaaatatcga aaaatttgta attatctttt
     1621 tttgttgaca ttgaacgtgg taacacattc acaggaaaca aaaaaatggt aataaaaatt
     1681 tttcatcaat tattttttaa gcgaaactaa atatttaaca atctccatcg gtacatcaat
     1741 tccagttgcc ttcataaaac ccttaaattg cgggccctta tttacttccc aaattaccgg
     1801 ctttttaaaa tcattatccc taaaggccac atctactccc gccaccgcca gtccgcaaac
     1861 ccttgccgct tttaccgcta gctttttaat ttcctccggc aaatttgcca cctctactgt
     1921 ccctccggca ctataattat ttctaaattc atttttagct gttctcgctc ttttcatcac
     1981 tcctaggact ttttttccca aaactaaaat tctaaaatct ccctcatttt caatgtattc
     2041 ctggagcata tatcttcttc catcctccgt ctcacttttt cttaagtctc tgaccagctt
     2101 ttcgagctcc tctaaattgt ttgccttaaa caccctcgtt cccctatccc caccgctccc
     2161 ttttatgatc accggccatt tgtatgggcg accttcatgg tcgcccttca tcacttcata
     2221 caaataccac aaagaaccat aaatactttt cggtacctca attcccgcct cttttaattt
     2281 cagcatttgc caagccttac acgcatagct cggctttccg tatctcacca aagggtctac
     2341 cacaattggc ccatgccctt ccgaagcttt gggcgaagga gggtttatac tattaataat
     2401 aagatctacc tcctcccagt gttttcccgt cgtcctaaaa aacaacacat catagtcatt
     2461 cgcgtctttt cctcttagag aaatttttcc cgtttccgtc tcaaaacaaa cctggttata
     2521 tgacaccaaa tctaaattta cccccatttt tctggccgct tctcgaacca aaaccaaatg
     2581 tttactaatt ttccctccaa aaaatactgc aattttagtc atatttttta atttttaata
     2641 aatttatttt tcccctcttt cttcaaagag gggttagggg tgatttggtt ttgtttagcc
     2701 gaaattaaaa atccttctaa atcttttctc ccgagtaaaa attttgttct gagtttttcc
     2761 ctatcagcta cattgaccgc cgtcatcacc ttttttccct tcagccaaaa acttacccca
     2821 attaccggtc tattcttatg tttacccaga cttgatttat aatgtcgaaa atataatatt
     2881 ttttccgagg acagtagtcc caatttttcg gccaaatttc tatcaattga gcttctaaat
     2941 gctccggtat ctacctttgc tcgcacctct atcactcgat gttttccctg tttaatgatt
     3001 gaactttttt gtaattccgg cttaaaaccc ttaggaattt tgagcttaat aatttctaga
     3061 ggttgaattg ttttatcctt ttctttttcc gataccttat ctgaaaattt ctctccaaat
     3121 aaatacttgg ccagagctat cccgtgttca tcgcttcgga ctgtaatatc atcaattttt
     3181 tcgattctac ttttaagtcc cgccttatta caaagctgaa tcgataggcc agatctggca
     3241 ttaacttcga gtatttttgg tccatcttct ttatcaatca ccacatctac tcccacaaag
     3301 ccgagacccg gaatcgctcc ctgacatttt acagccgtct cgagtacctc tttccaaaaa
     3361 ggaattttta ttccatttac cttaatcatc ttttttttgc caaaatcata tatttttcta
     3421 acttcaactc cctttccctc aattccaaaa gtggtaattc cactcgccaa gtcaattccg
     3481 aggccatatg ccccctgttg caaattagct tttcccccac tcttttcagt cggaatccgg
     3541 agcatggcca tcactggcac ccgattgtaa acaattaccc taatatcagg tgtccctgat
     3601 tttgttaatg ccaaaaactg cggatgaatt ttaattcttt cttccacgat taccccatcc
     3661 gccgttccgt gaaggctata tttccccgcc agtatttcga gacaatgcct cttaatctct
     3721 tctagactaa tcactctccc attcatcatt aaataattta tgctttcggc tttagaattt
     3781 agaatttttt tcctgactat tagtatcccc tctcctccat atcccgacac cggtttaatc
     3841 acaaaatttc cttctagctt gctccactca aaattttcca tttcttctcc atttttaaat
     3901 ctagctatca gtttagggac actaattccc tcttttccga gtcttctctt tgttttgtat
     3961 ttattgtccg caatcgccct tccctcagct gaattttttc ttaaatacac cttatttctc
     4021 tcatttttag ttaaaaactg tcgatattgt tttaaattta ttccgaacat atttatattt
     4081 tttctaattt ttaggcttta gaattttccg ccagaggcgg atcagcctct ggctgaagaa
     4141 ttttttattt taaacttttt tctttttttc taatcgctcc tttaaatctt tttatctcag
     4201 tcaatcttat tcccccatat cttccaacta aaacgttaat taccaccacg attaaaatta
     4261 ctatttctgc tctttttagg accattcctt caattccctc tacccccatg atcattgccc
     4321 ctaccactgc cagcactagt gtccccgccg tcaagctcac tgcctctttt ttgctcttta
     4381 tcagctgagt tctggcaaat tcttccgtca gtaagatcat aaagaggatt ggaaaaatag
     4441 aaatcttact aacatcaact attttaataa aagaagtcag gaccatcact ccaaaggtgc
     4501 ccagactcac aaataaaagc gtgatcgatc tagccggcca aaaatgtaac tttagttttt
     4561 tgaccaaaag attactcagt aaagtaatta ccagtattac caaaaaaagt ataagtccac
     4621 tccctactcc tgttgccaat agagcgaccg ccatcattgt tggcataaaa attccataac
     4681 ccgaaaaccc aatcacatag tgaatcaccg aaacaagagt cgccactaag ggtaaaagca
     4741 atagcagggc cactgtattt tccggcactc ctttactcac ggcctctgaa attaaatagc
     4801 taattccatt ccaaaactta aaaaccacca tccctcccgt ctccactcct tcctgagcgt
     4861 ttaccgctcc cgcatttata aacagccacg ccaaaaataa aagggtaatt tttctcatat
     4921 tttaggttaa ttaataatta attgaagatt gaagattgaa gattgaagat tgtagattgt
     4981 agattgtaga ttgtagattg tagattgata attgtagatt gaagattgaa gattgatttc
     5041 gtggcgtatt ataacaccat tttatcccta tatattgtat aatcttctta ttaatttatg
     5101 atgaacatat tactggccga agtcaatatt aaagacactc ctttaggtac tggtaacaat
     5161 cttggtaatg tctatacaac tcccgctagt ttaattactg ttatagtcag aaatagccta
     5221 actgtcgctg gaattattct cctcgttctt cttattgctg gcggttttat gatgattgcc
     5281 agcgccggct ccggtgatca aaaaaaagct gcaacctcca aaaccatgat taccgacgct
     5341 gctattggct tcttggtaat ttttctcagt tactttataa ttcaaattgt agaagttatt
     5401 acaggagtag atattctata aactatggca cttcctttta taggtccaat taataatccc
     5461 accaattatg gcaccgatac tgatagaggt cagggtctat tcactttctt aagcaatctc
     5521 ttcaagtttg ctgccgttgc cgctggtatt tatatgatta ttcagattat ccttgccggt
     5581 tttcaataca tcagtgcctc cggtgacgtc aaaaaaactg aagctgcctg ggctcaaatc
     5641 tggcaaagtc ttctcggttt agttatcatt tccgctgcat tcattatcgc cggcctggtc
     5701 ggacgcctta ccggaattaa cattttaact cctactatct atggtcccca ataaactctt
     5761 ggccgtcgaa tacgacctaa ttggtcctgg caaggttccc acctcaaatg ttcaggccac
     5821 caccatgctc gaaaaatata ttagcaactt tattggaatt ctcaccatcg tggccgttat
     5881 tttcttcgta attcagatta tttttggcgg ttatgcctat ctttccgcta acggtgaccc
     5941 aagtaaaatt gaaaaagcca ataaacacct caccggcaat ctcctgggac tactcatcgt
     6001 tattgttgcc gtcggtcttg gtggcctcat tgccaaactt ctcggtatcg ataatcctct
     6061 cgacattcag ttaatgttta ccaacctggg tttatagaaa aatatatgat tactaatcct
     6121 attctcaata atagccagct tcaggcttcc gatcctgctg cctacaccaa tagtgtcatc
     6181 cagaccattt tttctttttt tttaatcatt gccatcgttt actttatatt caacataatt
     6241 atggccggca tcagattaat cggcagcaac agtgactcaa aaaagatcga aacatccaaa
     6301 actcaaatta cctatgcctt cattggcctc tttattgtct tctgtatttt tgccatcctt
     6361 aagcttgtcg gtaccatcgt tggtattagc agtctcgaaa atttacaaat tacctggccc
     6421 accctttaaa ataatctata ttcctgttaa actttattaa tttattaaaa attcaaaaaa
     6481 atatgatttc ctcaatcaaa aacaaacttc aatatgccct cttagccatt cccgcctttg
     6541 ccgccactga aaatatcgac cttaccccaa aagataattt tgcaggcctg gctgatttaa
     6601 gttttggcgg tatcgtttca ggtgccatct ccttaattct tttagtagtc gctctcgtct
     6661 tcttttttgt ccttatcatg ggcggtttaa gatgggttat gtctggcggc gaccaaaaaa
     6721 atgtcgaggc tgctcgcaat caaattacta atgcccttat cggtctcgct attgtttttg
     6781 ccaccttcgc cattatgaaa ttaattgaaa ctatttttgg tatcaccatc ctcagcaatc
     6841 tgactatcaa gcctttttaa gataattcga attgaatcca aaacccccca aggtaacatt
     6901 ggggggtttt taagttataa ttaactcatg aaatctattg tcactttttt cctttgtctc
     6961 gtcatagctt tttgcgtcgg cggatctatt ctcccttctc cggtcaatgc ccaaacaacc
     7021 aaagattggg ataagcagca gcctggtcgt tgtgtaaaaa cagttaatat caatggccaa
     7081 agcttcgatg acatcgccac tatccagggt tttgaatgtc ttttctacaa cgtccttcag
     7141 attatcgtta ctattgccgg cctcgccttt ctttttatgt ttattagtgg tggctacaag
     7201 tatctcttta gtggcagtga atcaaaagcg gttgccgctg cctcttcggc tataaccagt
     7261 gcttttatcg gtcttatcgg cgttattgct tcatggctta tcttaaaatt aattcaagat
     7321 tttactggta tcaacgttac tatctttaaa attcctggca atccgtgaaa aaaaatattt
     7381 tattatttca cgtttcccgt ctctcgtttc tcgtttcatt catgtttttg tttgccattc
     7441 cttctcacgc cttcgatctt ctcaattttc tttttccccc cgttcaaata gaggaaagcg
     7501 agcgccgagc tcctgatccg gcattagctc cagaagttcc aagcgacaat acttattcca
     7561 atactcttga aaatgaaact attacctgtg gtggaaatgc tagcgctggc gacagctggt
     7621 caaaggaaga tcataaaacc gaagttactt taatcgactc agaaggtaac ccttatgctg
     7681 aaactcaata tttagattac aatgctgaaa atctcgacgg agaggttgat ttaaaaaatc
     7741 tctccaacga atattatcgt aattttcaat ctttttttgc ccgcggtagc ataaaatgta
     7801 ccgaagaaaa ggccaaaaac agttttcttt ctctagaaat gaatggctct agcgcttccc
     7861 tccgttcttt acccacaaat caaatatatt attatcgcag tctattttta actgaagccg
     7921 ctcaatcttt aaacaatagc caacagttcg acaccgttac ccaggattat caaatcgcct
     7981 gggactgtcg gggtacctgt gtcgaaatgt ctcaggataa atcaaaatta gattctgcct
     8041 gccgccctat ttatctgacc gaaattattt ttggtcttca aaatgaagcc ctctactact
     8101 caagtcccac tgccaatccg gaattttttc ctgcagagct cctcacccct gttttaagcc
     8161 actacagtgg tagttgtggc aattatggct gttattccga caggtcagag ggaaaagcct
     8221 tcatccccct aaccaaagaa gattatcttc ttttttacaa acaactcaat tatgttccca
     8281 agggtaatgt taatagtcgt gtcaccgtta ccaactataa tggttacgat accggtagcg
     8341 aaacccccat taatccggtt gatgaagttt ataatcgaac cctgcctaat gccgccgccg
     8401 cctattctcc ccactccacc tctctcagtt ttgtcaatcc caaccaacag aaacctcttg
     8461 aaaacagtga catgtgtgaa aatagtaccg ttagtccctt aacctcccgc gattcacccc
     8521 agcccaatac cattactacc ttcgttaaag gattaatcaa acacgttctt gccggattcg
     8581 atttcagcaa aagtgtccct attccagtta gcaccaccta cgacaaaaag gtggttgaca
     8641 atgtcaaaat tagcgagcag gccttttcca acatgattcc ctcaaagtct cttgaggaga
     8701 aaaaattgct cgaagtgaaa ttttccagca aaactcccgc caatgacgag ccaaacgtcg
     8761 ttgatcctgg ttttcgttcc gatcttctct atcaagaaat gcgttccctg cttcgcccct
     8821 catcttggta atattttgct acaataaatt atggccaata attttctaaa gaaaatttta
     8881 ttattaatct ttttctcttg ccttgtaatt tttccccatc aagtttccgc ccaagaagct
     8941 cccactgctt caaacaatct cttttctcaa aaacaaaaag ctattcaaaa taacaccggg
     9001 cttgaatcct ggacaaacga ggctatgagt agcaacatgg tttctctctt tgttggtatc
     9061 aatggtaccg tccccgagga ggtactcaat gacgacgctg attttattaa tcattcttac
     9121 atccccggag gggctatagg tcaactcaac gaggctattg ttgccctcca cgagcctccc
     9181 gcctcaggaa tcgaatatat tgcccaggta cgggataatt ttcttggcaa accggcttat
     9241 gcccaaaacg gttctttcca aaaactccag tttatccttc ccctttggcg catctttaga
     9301 aacgtagttt atcttctttc tacaattttg tttattgccg ttggtatgat gatcatgctt
     9361 cgagtaaaag ttagtcctca ggctgtcatt actgttcaaa gcgctattcc tcgaattatt
     9421 actgttttaa tcttagtcac cttttcctat gccattgccg gactagtttt agatttatgc
     9481 aaccttttcc aagccattgc tacctcttta cttttttccg cccaaaacaa gggacttacc
     9541 gagaatctgt ttgaagatac ctactggtac caacatggtg tctcggattg gtgggaatca
     9601 ataaaaaatc ttctaggtgt tgacaagcca aattttacct actcaagtct tgtcaatctt
     9661 gattccgcca ccacaagaga tttagcaaaa agaatctccc caaacgtaac tactggtcat
     9721 tatttaggct tattcattgg aaaagttacg ggtgttggtg gtcttttggg tggtcttggt
     9781 ggtgtcttac taccattatt aatttccatt attaagtttt tttggttgtt taagttgttt
     9841 tttggtttat taaaatgcta tgccaacctg cttataaaaa ttatttttgc ccccctcgag
     9901 attgccatgg gggctcttcc aaactctaaa atgaattttt cttcttggat taccgacttt
     9961 gctgccaatg ctctggtttt cccgattgtt gccttatttt taattgtaat caatgttatt
    10021 attgacgtct ttgttgctgg aacaacaaat caagcttttt ggatgcctag cataatcgat
    10081 ggcgctagac tctttcatta caaagaccaa gcttacaccg ctattgctac cggccttggt
    10141 cttattggcc tctctctttt aagcaaatta cccgaaatga ttcctgttat gattaaagaa
    10201 atgaaaggtc ccgattttgg taaggctata ggtgaa
//
LOCUS       DHGB01000055           13565 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_56499, whole genome shotgun sequence.
ACCESSION   DHGB01000055 DHGB01000000
VERSION     DHGB01000055.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 13565)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13565)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13565
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_56499"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    1042..1092
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6409..6514
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4008 a         2672 c         2283 g         4445 t
ORIGIN      
        1 atttgaaagc tcaattaaaa attattcaac cgaaaatcgt tattactttg ggtcgttttg
       61 cgatgaacta tttctttccc caagaatcaa tcagccgggt tcatggacaa attagaaata
      121 ttacttggca ggatttacct ttaactatta ttcctgtcta ccatccttcg gctggcctca
      181 gaaacggtgc catgcttaaa tctctacaaa acgatttcct tagtattggc aaattactaa
      241 aagataacta gccagtttaa ctatttatct tttttactat ttatctatta aactactaaa
      301 ttaaaatatg attaatcaaa aaacaaatca aaaaccggct tttaatccag ctgttcaaaa
      361 ggatttctct cattcactta gtcaagatgc tctacgcatc tcttctcttg gaggttttgg
      421 tgatgtcact caaaacatgt tcgtttacga atttgctcct cggggtgatt tttccaaatc
      481 ccaaattatt atcgttgatt gtggggttgg ctttcctgaa gaagatgcct ttggggttga
      541 tcttcagatt cctgatacca cttatcttct tgataaaaaa gaccgtatct tgggtatttt
      601 tatcacccat ggtcatgaag atcatatcgg tgccgtccgt tacattttac cgattttggg
      661 aaaaaatatt cctatttatg cgcccaaact agcggccgcc tttattgaag cccgtctggc
      721 cgaaaatcaa attcgagcct cggttaatgt ttatcagccg ggtgatacct ttaccaaagg
      781 tctctttact attgaaccaa ttcgagtgac tcactctatt cccgatactt atcattttgc
      841 catcaatact ccacttggta ttttttatca tggttctgat tttaaatttg atgtctttcc
      901 tttagataat aaaccagcag aaattaaaaa aatcgccaac gttggcaatc gcaaagtagt
      961 ggctttaatg tctgattctc tcggttccga tcatgaaggt tattcaccct cagagcggga
     1021 tattgccgaa aactttaagc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnggttattc tgtggataaa gccatcaata ttgctaaaga actgggttat
     1141 attaagcttg acgattctga tcttattact ccggaaaggg caaaaaactt tcccgacaat
     1201 aaactattat tcttagtggc tggttttgcc ggtcaaccag actcagctct ttccaaaatg
     1261 gttatgggta aacaccgtat aaaaataaaa tcaggcgaca aagtaatttt ttctgctcct
     1321 gattatattc ccggtactac cggcaatatc tacgaaatga ttgatatcct ttccaaaatg
     1381 ggcgccgatg tggcctatgg tgataaagaa gaactccacg tttctggtca tggttatcaa
     1441 aaagaacatg cgcttttaat ttctttaacg aaccctcgct atttactacc aatcggtgga
     1501 aattatcgac atgtcaaaag ttatttttcc atggccaaga ccatgggttt taacgatgat
     1561 cagtttatta cgcctgatta cgattctgcg gtcacttttt ataccagtgg taaagtcgat
     1621 actaattttc acattccgat gcacaaagtt ttaatcgatg gtcttggcat tggtgatgtc
     1681 ggcgccaccg ttcttcgtga tcgaaaaact ctcgccgaag atggcatgtt tagtgttgtt
     1741 ttaatggtta ataaagaagc cggcacgatg tttaaagaac cgataattct ttctcgtggt
     1801 tttgttttta tgaaagaaaa tactgactta atcgattatc taaaagaaga agttttaaga
     1861 aaatttaatg aagttactag taaaccggcc aattttgatt atattcggac ggaaattcaa
     1921 gcttatctcg aaactattat caaagaaaaa accggccgtg agcctatggt tttaccattg
     1981 atcatcgaga tctgaaaata tgcctcgtat cctaaattaa ttatttaaaa ataataatct
     2041 gttaaaatct gaataagctc gtctttgtct caggatttat cccaggcatg gcgagcattt
     2101 ttgttgttaa aataacttat gaaatactca caattatttg gcaaaactag caagaccgtt
     2161 gcttccgatg ctgatagtat taacgcaaaa ttattaactc aggccgcttt tattcaaaaa
     2221 caaatttccg gtgtttacaa ttatttacca ttaggtttga gggttttaac aaagattcaa
     2281 aatataatcc gtgaagaaat gaataaaatt gatggtcagg aaattttgat gccggccatg
     2341 actcaagagg aaagttggat tactactggt cgcaatacta tggaggttct ttttcatctc
     2401 aaaggtcagg gtgatactaa tttagtttta aatcccactc atgaagaagt ggttactccc
     2461 ttagttcaaa aatatgtatt ttcttatcga gatttacctt tggctgttta tcaaattcaa
     2521 acaaagttcc gaaacgaggc tcgggccaaa tctggtcttc tccgtggtcg ggaattcaat
     2581 atgaaggatc tttattcttt tcatatcgac gaaactgatt taaaccgtta ttacgagatt
     2641 gttaaacaag cctattttaa aatatatgaa cgaattggta ttggtcaaga aacggtatta
     2701 accttcgctt ccggtggaaa tttttcccaa ttttctcatg aatttcaaac tttaaacgaa
     2761 ggtggcgaag atactattta tttatgtccc atttgtcgtg tcgctgtcaa taaagaaata
     2821 attgacaccc aaaaaacttg tcctgaatgt ggcagtgata aattggaagc tgtcaaagcc
     2881 accgaagttg gtaatatttt taaattaaga actaaattct cagaagcttt taagttttat
     2941 tattctgatt ctcagggaca gaaaaaaatt gtggaaatgg gttgttatgg tatgggtccc
     3001 tctcgggtta tggggactgt ggttgaacta cgtcacgatg acgtcggtat tatttggcca
     3061 aaaaacattg ccccttatca gattcactta attggtcttg acggtctcgg tgaagattta
     3121 tatcaggaat tactgtcaaa aaatgttgat gttatttttg atgatcgtga aattagcgcc
     3181 ggagcaaaat ttgctgatgc tgatttaatt ggtattccta ttcgtcttgt tatcagtcaa
     3241 agaagtcagg aaaaaggcgg catagaattt aaattaaggt cagaaaaaga atctgaaatt
     3301 attgaagcaa aagacttggt ctctaagatt attaaattct ataattaatt tattgatttt
     3361 tagagtttag actttttcta tttttactct atactgtatt tatgcctcgt ggacgcaaga
     3421 aaaaccgaat tctcccaact ctaaaactta aaccagatac cgttaaatca atcttcttta
     3481 ttttctttct aatattagcc atattatcgg tattttcctt tcttcagcag gggactattg
     3541 ctactgaggt taatttaatc ttaactaaat attttggctg gggagcagtc atggttcctt
     3601 ttttatttct tttaatctct tttctcttta ccaaagtgaa atctgctctt cgtgaagcca
     3661 atgtcttttt tggttttact attatgtttt ttgctcttct tggccttttt aagcaaggct
     3721 ttctcggtgc ctttttttgg gagcaatttt tggctctatt ttctgaagtt ctcaccttta
     3781 ttattttctt ttttgcccta attgtcggct ttgtagttct ttttgatacc aatgtcgctc
     3841 agatttttaa attctttgct catctttttc aaatgattaa aaagtattct attggtcaaa
     3901 gtaataaact aaaagataaa aaagcgaagc agttagccca aaacccaatt taccaatcag
     3961 aaatgaactc ttcagccaat tccgaattaa atttaagccg ggataaagat atctcaaaat
     4021 caagtgccaa ggccgacttt ggtgttgtca acattcctcc ggttatgaaa aattcttctc
     4081 ctcttacctc ttctggcaat cagacctggg tttatccttc tctttcaatt tttgatgaca
     4141 ctcccggagc caaggccgac aggggagatg ttcgtaaaaa tgcccagatt atcgaaaaaa
     4201 ctctcgattc tttcggaatt acggctcggg tagctgaggt taacaatagc ccttctgtga
     4261 ctcagtacgc tctcgaagtt tctcttggta ccaagcttgc caaaatttta tctctcagta
     4321 atgatctggc tatggccctg gctgctcctg gtggtcaaat tcgagttgaa gcacccattc
     4381 ccggtcgctc tctcgttggt atcgaaatgc caaatcgttc tctagaggtg gttcccatta
     4441 gaactgtctt ggagtctgag gccatgaaaa atgccaaaag taaaatttcc gttcccctcg
     4501 gtctcgatgt ggctggtcag gctagaattg ctgatatttc taaaatgccc cacgttttaa
     4561 ttgccggtca aactgggtcg ggaaaatcaa tctgtattaa ttcctggatt tcaaccttac
     4621 ttttcagggc ttctcctgat gaagttcgtc taattatggt cgatccaaag cgggttgaac
     4681 tttcacagta caatggtatt cctcatctac ttaccccagt tatcgttgaa ccagaaaaag
     4741 ttgtttctgc tttaaaatgg gcggttaatg aaatggagca tcgttataaa ctgtttactg
     4801 aagttggagc caaaaacatt gaaggttaca acaatatgtc cggttttcaa gccatcccct
     4861 atattttaat tataattgac gagctagccg aaatcatgct tttttctccc gccgaagttg
     4921 aagacaacgt ttgtcgcatt gctcaaatgg ctcgggccgt cggtatccat ttggtcttgg
     4981 ccacgcagcg gccctcagtt aatatcatta ctggtcttat caaagccaat atccccaccc
     5041 gcattgcttt tgctgtagct tcaatgaccg attcccgggt tgttttagat actcccggtg
     5101 ccgaaaagct tctcggtcgc ggcgatatgc tctatattcc ccccgattta gccaagcccg
     5161 ttagaattca gggatgcttt atttctgaca aagaggttaa tcgcttaatt gattttctaa
     5221 aaactcagcg cagtggtgat tacaatgatg agattacaaa acaaccagtt agcagcccca
     5281 attcaatcag taaaaatatt atggtcagtg acggtcagga acgagatgct gtttttgacg
     5341 atgctgtcaa gctgatacaa gacactggta aggcctcagc ctctttcatg cagcgtcatc
     5401 ttaaactcgg ttatgcccgc gccgcccggg ttctcgacga gcttcaagca gctggtattg
     5461 ttggtccagt taatggtgct aaaccccgag aaattttaat tccccataaa agttcttccg
     5521 ataatagttt ggaaggataa tcgatatgaa acgtgcttca gctattctcg agtcagctcg
     5581 ccttgatcgg gaaattgatt ttgaagaaat atgcaaaaaa actaaaattc ctctccgtta
     5641 tcttatcgcc tttgaaaacg aaaacactaa agaatttccg gaggagccct actgttcttt
     5701 gatggtcaag gattatgctc agtttttagg tttaaatgga gacgaacttc tctccctttt
     5761 tcgtcgcgat tacaaccggc acttgccaac aaactcaacc cctcgttcct tattttcctt
     5821 aaccccccaa tttacctttt ctttttttat tgttttactt tcactttctt ttgttgttta
     5881 tttagtttca gagtatttaa aattcaatcg tccaccccct cttgaagttg tctggcccga
     5941 gaatttctcc gagtcttcac tcaaaatatc cggaaaaacc gatccccagt cgaccgttaa
     6001 aataaatgat actttggttt tagttgatat tgaaggtaat tttaccaaag agattcaaat
     6061 ttctactccc gaggctaaaa ttgtcattga agctgaatct cccgctggca aggtcaccat
     6121 tgatgaaaaa gttctaaaat agtctatact attcctatgg atatcaatca aattgtcatc
     6181 attatctctc ttttatctat ttctactgtt gttgttatcg tcggtattta tcttatcaag
     6241 ctgataaaag atttaagggt aaccgttaga gaaactaatc aaatcatttc ttctgtttct
     6301 cgtcctgttt cttctttttc agaattttta atgggtttca aaaatggctt ttcccttttt
     6361 aattcctttt ttaataaaga taaaaaagaa aaaaaagata atagttaann nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcaagc ccaaatcaga aaaaataatt
     6541 gaatctataa tcactcctga tatccccaga aaaaaaatta tcaaaaataa aaagaaagat
     6601 gttgttattc cccaaaacct cctaatcgcc gacacgaccc caaaaccaac tttttccaaa
     6661 ccaaggaaaa tgtttaaaaa ataacgttac cttcttgcta taattcctct atgaatgcca
     6721 ggcagttaaa agacatcttt gtaaactttt ttgtttccaa aggtcacgtt gaaattcctt
     6781 cagcttcctt aattccagag aatgactcga ctactctttt tatctccgct ggcatgcatc
     6841 ccttagtacc ttatcttttg ggtcaaccgc acgcactggg taaacgtctt actaatattc
     6901 aaaaatgcgt tagaacaggg gatatcgatg tagtcggtga caattgtcat cacacctttt
     6961 tcgaaatgct gggtaattgg tcactcggcg attacttcaa aaaagagatg attccctggt
     7021 cttatgaatt attgacgaag cacttacatt atgatcccaa acaaatccat attacctgtt
     7081 ttgccggtga tgacgatgct ccaaaggatg aagaatcagc cgaactttgg aaatcagtag
     7141 gaatttctga agataggatt tattttttac ccaaaaaaga caattggtgg ggtccagccg
     7201 gtaatactgg tccttgtggt cctgacagtg aaatgtttat tgatacccat ccagaactag
     7261 aaccgattaa gctaacgagt ggcgacaatc acggccgaat tgtagaaatt ggaaatgatg
     7321 tttttatgca gtacaacaaa accattgatg gaaaatatga gttactttct caaaaaaatg
     7381 tcgataccgg ttatggtgtt gaaagaaatc tcgctatata tcttggttac gatgatgatt
     7441 atttaactga aatttggcag ccgattatta acaaaattga agaaatttca gaaaaaaaat
     7501 attctgaaaa tttagtccct atgcgtatta ttgctgatca tatcagagct gccgttttta
     7561 tcattgcgga cggagttgag ccgagcaata aagaagctgg ttatgttctt catcgcctca
     7621 tccgtcgcgc cattcgtcag ggaaaaattt taggtctaga aaacaatttc actagtatca
     7681 tagcgaaagc tgttttagat aatcaaaata attatgccgg tgtttatcca gaattgaatc
     7741 tcaataaaaa taaaattata gaaactttag atgccgaaga aaccaaattt cgtcgcactc
     7801 tcaataaggg tcttaatgaa attatcaaat taattgactc ccaaaaaagt gtttctggca
     7861 agcaggcttt tgatttatat cagtcatttg gtttccccgt tgaaatgatc gaggaggaat
     7921 taaaaaagaa cggtttatcg ttagatttag ccgaatttaa tcgacttaag ggcgagcatc
     7981 aaaaacaatc tcagactctt tcagctggta agtttaaatc tggtcttgct gatcagagcg
     8041 aaataattac caaatatcac actgccaccc accttttgca tgcggctctc cgcaaagtgc
     8101 ttggaaatca tgtcaagcaa tcaggttcaa atattacttc cgaaaggctc cgcttcgatt
     8161 tcactcatcc cgaaaaagtg acggacactc agttaataga agtcgccaca attgttaaca
     8221 agcaaataac tgcttcactt ccggtcactg ttgaatcgat gaaatttatt gaagctcaaa
     8281 aaattggagc cctagccttt tttggtgcta aatatccgga aattgttact gtttatacta
     8341 tcggtacttc cggccgttac ttttccaaag aagtctgcac tggccctcat gttgaaaata
     8401 ccggtatttt aggtaaattt gaaatcatca aagaagaatc agccggctcc ggtaaacgcc
     8461 ggatttatgc tatccttaaa taatggagct ctcctattat tggctcattt ctattactac
     8521 tgaatctgtt accgttggtc taatcaagcg ggataatcca gatatcctag tagctctcgg
     8581 tccggaaaca gaatggctga gcggtaaccc agactctttt cttcactcta tcgatatttc
     8641 tttatctgag gccgctgaaa aagctgggct tgagccagac caagagcctg aatctagtgc
     8701 ttttattttg cctccaagtt ggattggtaa tgatggaaaa atttttcctg aaaatttaaa
     8761 acttttagaa aatctatgtc attctcttga gcttaaacct cttggtttta ttagtagcga
     8821 tgaggctttt attgaatcag tcaataaaag cgattctttc cctcccagct ttatccttgt
     8881 tcatatcggt aaaaaagaat tttccctctc cttggtttat cttggtgaag tcaaaaagag
     8941 attaagtcaa aagctaatta atgattttac cccagaggat ctcgaaaatg ccctacttac
     9001 ccttcaatta gaatcagctc tcccacccag aattttgatt tttggcaact taggtccctc
     9061 aattatcgat gatttaaaaa attattcctg ggtgggtaaa aaaaatattg aaactttcct
     9121 tcatttaccc gacgtaactg cctttgaggc gcaggaatta tttagtcttt atagtcaaac
     9181 tgtttcttcc caaattagaa atgatgccgt tgaaaccaag cctgaagctt caattgttcc
     9241 accagctcca cccgccgaat caaaaaaccc aatcgaagaa atcattccac cagttattga
     9301 ggtcgaagct atttctccgg aaattgagct ttctccaaaa gagctcggtt tttctgagac
     9361 cattgaatcg ggtgagccta aggtcaaaac taaattttct ctacccgctt tttcttttcc
     9421 ctctctaaag cttccccatc taaattttaa tccctggcta cttttccctt tagctctatc
     9481 accactttta attttaattc ccttctattt aataaaagcg gaaattacca ttaattttag
     9541 tcctatcgag ataaataaag tttttgattt aactttagat tcaaatgcca cgtcaatgtc
     9601 tacttctctt ctccccgccg acaaaaagat aatcgattta aatttttccg aatctatttc
     9661 caccactggt caaaaagaaa cgggggataa ggccaagggt gaaattacca tctttaataa
     9721 agccgacaag atccaaaata tttccaaggg gactatcatg acggacaatt caaataaaaa
     9781 atatgaacta ttaacctcag ttcaggttcc agcctcctct tttaatttag gttcggggac
     9841 tatcaccatg ggacaaacta aagccaatgc tattgccagt gatattggtt ctgagtttaa
     9901 tttaaataag gatactctcc taaattttaa agatgatgct aatcttctcg ccaaggttaa
     9961 cgagtctttt actggcggct ctcgtcgcca aatccgggtc gtgagtgctg acgataaaaa
    10021 taatttaacc aaaactgtcg aagccgccat tgagtctcaa atttctcaaa aagtcaacgg
    10081 taataattct tatcaggatc ttctaagaga cactttattt atcgataaaa aacaactaat
    10141 ttttagtcgg gaaatcggtg aggaagcaga aactctagaa gttaaggcaa acttagctat
    10201 ttctttcttg cagattaatc ctcaaaaaaa agatgatatc atcaaaactc ttcttgccag
    10261 tgaccaaaat tttctccagt ctgttgcttc tccgtctgat ttttcttttt ctttttccgg
    10321 ggtcaaaaca acttctcaaa aatcaactgg acagcttacc ctaatcggta aaaccttacc
    10381 taaaattgac tctgatcgtc tccggactct ccttgcgggt aaagattttg ctaaagccca
    10441 aaaaatttta gattcccagc cttatgtcta tggtcgggaa attaaaataa ttcctcaagt
    10501 attttccttt ttgaaaagac tgcccccttc agccgacaaa attaccatta tctctaaatt
    10561 ctaagatgag ctacctttac gtaaaaagtc ctccaagaat tagcaaagct aaaaaatctc
    10621 tttctcctca tcataaaaaa ataattgctc ttttttttct tttttcggga atatttttgt
    10681 ttctctctgc tattttacct attcttcagt ttcaactcag ttattctact aagttcaatc
    10741 aaattttaaa tcctctttct ccccagtttt ataatcattc ggaatcagtt ttgggtgacc
    10801 taaataccga ttatacccag ctgtcaaatt ggtttgaaag tgaccctgac caaagtattt
    10861 cttctgcttt gccaaattat tctacgtctt cctattacat ttccattccc aagcttaaaa
    10921 taaaagaggc tgaagtcatt tttggaagta ctgatttaaa gaaaagttta attcattacc
    10981 ccaaaaccgc ccttcctggt cagtacggct cacccgttat tttcggccat tcggttttac
    11041 ctcaattttt taatccggag tcttatctga ctattttttc cactctttac agattaaagc
    11101 agggtgatga gatttttgtg aattacgatc aaattcagta taaatattta gttgaagaaa
    11161 tgtttgaagt tcaacccacc gatctttcgg tcttggagca gcgatttgat gatcgctact
    11221 taactttaat tacctgctcg cctccaggca cttatctgcg tcgtttaatt attaaggctc
    11281 gcctcactga taacacatga actgcctggc aattgacttt ggtaccaaaa gaattggtct
    11341 agcttattca ctaaacaaca ttatttttac cttacctata gttaaaaatg ataacaaaat
    11401 ttttgaaaaa attaatcaaa ttattatcga aaataagata aataaagttt atgtcggtct
    11461 ctcagaggga aaaataagtg aaatgactaa aaaatttatc aaaaaacttt caggcgtgat
    11521 aaaattacct atcgaaacag ttgaagaatc tgtttctaca attgaagcta ataatctttt
    11581 tcttgaaaac aaaaaaaagt taaaaaatta tcaacaagaa attgattcca tggccgccgc
    11641 cattatttta aatcgggtta ttaattctta gtcagcctat ggctgaaaaa aatatgttta
    11701 aaaatctaat ttctccaatc caggcttggt tattatccca aggtcgttgt gttggttgcg
    11761 gcgaagaact ttccaaaggt caaagaaaag tggttcgggg acagaccacg gttacttgtc
    11821 gttgtgggcg tatctttatc cataatcacc agactgacac ctatcgccgg gctcttttca
    11881 atgaaattta aaaggggatt aatcgccatt tttattatta tttttgccct agtattttat
    11941 ttttatcttt ctctcttgcc tccatcctct gattcatcta aaaaagtatt tgttatcaat
    12001 gagggtgact ccctttccac tattgctcta cgtttagaaa agaatcaatt tattaaaaac
    12061 cgttatacct ttattattta tgcctattta cttagcttaa atcaaaaaat tcaagcaggg
    12121 actttttatc tgacttcatc tcaaaaaatt cccgatttaa ttaaaaatat cagtcgtggg
    12181 ggcagcactg attattggtt aaaaattatt cccgggacca gaattgaaca gttttctccc
    12241 agttccgagt ttacccttat cagccgagat ctcgagggta aattgttccc tgacagttat
    12301 ctgattccgc aggactatac cccagaaaat attttatctg taatcgaccg aaattatcag
    12361 caaaaattat cacaagctcg gcttggcgct accactaaac attccgataa tgatgtctta
    12421 attattgctt cacttttaga acgtgaagcc aaagctttag ctgataaaaa aattattgcg
    12481 ggtattattg ataatcgtct cagtcgccag atgcctcttc aaatagatgc cactattcag
    12541 tatgctcggg attctctcgg tgacctcagt gattattgga aacctattag caaagcggat
    12601 attaatataa tttctcccta caatacctat aaaaataaag gtttaccccc cacgcctatt
    12661 tgtaatcccg gcctcgattc cctaatcgcc gcttatcatc cagacaggac tgattacctc
    12721 tattatattc acgattcaaa cggccaaata cactacgcct cgaccctgaa tgatcacaac
    12781 gacaacgtgg ctaaatactt aagataaaac cttattaaga atttctaatt tatttttatc
    12841 tggccttctt tttaattttt ctatatgaag caattcaatg ggatcttggc agagttttgg
    12901 tatatttaaa atacatcttc cgttatataa aaaattgcat tctttcgaat attgactaca
    12961 aataacacaa ttttcaccta ttaaagcagt taaaattagt ttattttcag tcatggcgaa
    13021 ttgtatcact aattatagta ttttagacat catttattct tctttcttat ttacgaggtc
    13081 atttaaaata ttccagcgtc tttttttctc actcatcggt atttcatctt tatataattt
    13141 tgccgaaaca gttccccggc ggggggagta tttattaaga taggcaatat caaaaccaac
    13201 ttttttacag acatcaactg tattttgaaa ggccacctca tcttcgcctg ggaaaccaat
    13261 aatgatatcg gttgaaaact taactccggg aactttgctt tttatttttt ccactaaatc
    13321 caaatattcc tgtttcgtat atcctcggtt catatttttt agtacttcat catcacctga
    13381 ttgaaatggc aggtgtaaaa gcctgtcaat gttggaatat ttagcaatta cttctatgag
    13441 ttcgtcggaa aaatcccagg ggttactact gataaaagac actttcttta aattcttttt
    13501 tgctacctct tctagtagct tcgggaaact tggttgataa gaatttacat tttgccctat
    13561 gagta
//
LOCUS       DHGB01000056            7403 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_39875, whole genome shotgun sequence.
ACCESSION   DHGB01000056 DHGB01000000
VERSION     DHGB01000056.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_39875"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         2190 a         1228 c         1576 g         2409 t
ORIGIN      
        1 gattttttaa tggttgattc ggctaaaagt tgtactttgc gatttagtga aagctggata
       61 ttttcgccat caacttcatt tttttgccaa tttttacgag tcaagaggtt ttcccccact
      121 ccatcaacaa tacttctaat aaaaccgatg cggcccgata ttttgcgttg ataatatctt
      181 tcgaggttaa cgataatatt tttggctaga tcattttctg gataaaaacg ggattgcttt
      241 agggtaaaat caagcccagg tatggaaaca agacttggta tttcttcacg gctaaactgg
      301 cttttaaact ccacccattt ttgagccgga ttattataaa tttccaatat cctggcattt
      361 tcagtggcaa aggtaggttt tattttctcg atttcggcga gtacctcgga gagttcttgt
      421 ttaaagttgg gtttataaaa agatagggta tagtaagtgt aatcgagact aaggggaaaa
      481 gaatctgagg cgaggagatg tccctgctgg ggaataattt tttcaagacg ataaagctga
      541 gaagtagctt tatcacgtag ctcctgacct tttattattt gccagtaaaa taatctggcc
      601 agaataataa caaaacaaag atagatagta atcaaaatcg ttttagaacg attcgattcg
      661 gttgacggcg tttttttggg catagtttat ttctaatttt tcgttttcat atttaagatc
      721 agtatatttt ttactcagct cggtataaat ttgattttcg gcgactattt ggctggaata
      781 gtagagagaa aaggcaatcc ggaagacaaa aagtgctagg gcaatagcga ctaaaatacg
      841 atttaacatc aattaagatt ataaagccta aggatggcag agcgagaaag tggatttttt
      901 ttaacttcac tatgagtggg taaaaatttc ttggagagat aattattttt ttgaagatat
      961 tgtttgacga ggcgatcttc accggagtga aaagatatta tggcgacttt accatcggat
     1021 ttaacaattt tgggaagagc agtcagcagt ctattgatat tttcaaattc gtgattgaca
     1081 gcaattcgga gagctaagaa tattttagta gccggctggt gatagcttga ataatgtttt
     1141 ttttgataat atgaactgat tatttgactt agctccgctc catttttaat aggttttttt
     1201 tgtctggctt taataatttc gaaaattaag ggcttggcta gtttttcctg agtatatttg
     1261 gtaaaaattt gataaagctg gtctttatcc caggtgttga taatctcttc ggcagtcaac
     1321 gcttgtgttt tgggatttaa acgcatgtca agcgaagagg tatctttaaa tgagaatccc
     1381 ctgctttggc ctgactgttg atttaggcta agaccgagat cagctaaaat tccatccagt
     1441 ggttgattaa tttctttttt ccagatagta aagatacggt taaaattgcc aagaagtgga
     1501 tggaaatttt gagagagacc gaggtcatta attctgtcag cggcgatttt tagattaaga
     1561 ggatcagcat caagacccca gacaatcgct ccttttttta atatttcaag gctatgaccg
     1621 ccgtttccca aagtaacatc aaaatatact tggcctggct tagggtcaaa aaaatttata
     1681 atttccgggg caagaacggg ctggtgatag ttcattaaag ggcgccgcta ctggtaacaa
     1741 ggtagccgcc gtcaagataa tttttgtgag cgatataaaa atgagtgccg cttggcttgt
     1801 ttttaggagt gtaggtataa ttttcttcag tccaaactag actgccaccg gcttttttta
     1861 aatcagccgt actttgaaga ataaagggac tttcaaacat ggacatttgt ccgtttttta
     1921 aagaaaaaac aagacttaat ttaccacccc taacgccagt atcatatttg tatttgcagc
     1981 cgttggtaca actttcagtg cccaggagga ttttacgttc tgatttaccg gtggataatt
     2041 cctcagcctc aattatgcca ctggcacctt tttcaatttc gagattatca tcagtggcta
     2101 gataggtaat ttcatactca attttgctga tagtatcaga tattttgttt atttttaaat
     2161 atagctcatg gccatcggcg cggggactaa gggtaatttc gggtcgaaac tgaacttcca
     2221 tttctaccag ttttggagcc ggggtgggag aaggggcggg ggcagttttt ttaccacagc
     2281 cagacaataa aaataacgaa aggacgacga gaattaaata ttttttcata atgggtctag
     2341 tttaacttat ttgagagcca ggagtcaatt tcctcaattt taattctgat ttgggacatg
     2401 gtgtctcggt cgcggatact gagagttcca tcttcgagac tttgataatc aataacggcg
     2461 caccagggag tgccgatttc atcttgacgg cgatattttt tgccaatatt actactgtca
     2521 tcaaaatcga gagagtattt tttagataag tcagtgtaaa gattttcagc taaacttacg
     2581 agctctggtt tattggcggc gagtgggaaa atggccattt tataggcaga aagctttggg
     2641 tttagtttta aaaagattcg ggtttcacca ttgtcgagtt tttgctcctg ataggcgtcg
     2701 aggagaagga agaaaagaga gcggtcaact ccccccgaag cctcaacaat atgggggata
     2761 aatttcttgc cggtagttcg atcgagataa cttaaatcct gatgactata tttggaatgc
     2821 tgggtaagat cgtaatcccc tcgccaatga atcccctcca tttcagacca gccccaaggg
     2881 gcctcaaatt caatatcaaa tgcttttttg gcgtaaaagg ccagttcatt tttctggtgc
     2941 tggcgaaaac ggagagattt tttttgatta aaaagccctt gataccaggc cattcgagtt
     3001 tctttccagt attcgtaata ctgattcata tctttttcct gacaaaaaaa ctgaatgtcc
     3061 cattgttcaa attcgcgggt ccgatagaga aaatttttag gagtaatttc attgcggaag
     3121 gctttgccga tttgcgcgat gccaaaagga attttgaccc gggaggaatc aacaatattt
     3181 ttaaaatcca gataaatatt ttgacaggct tctccccgaa gataagcggg gattttttca
     3241 ccttcaataa ccccgatttc ggttttaaaa agaatattaa attttttggg attagtaaac
     3301 tctcccccac attcaggaca tttagtgaca ttatcgatta aatcgggtcg gaatctttgg
     3361 tgacaggatt tgcattcaat gagttcatcg ccaaaattct tgacatgacc ggaggcttcc
     3421 cacactttag gatgggtgat gatggaaccg tcaatgagga cgacgtcgct tctctcctga
     3481 acattatttt tgagccagag tgatttccag ttatttttta aaagagagcc aatgggaccg
     3541 tagtcataaa aaccctgaat ccctccataa atttctgaat tttgaaaaac aattccccgt
     3601 cgtttacaga gggagacaat tttggcaact aattcatttt gttcgcttaa cataagttat
     3661 tataaccgat taatgaaaga gtttatggct gtaaaggggt ttttcaatta ttgattcgca
     3721 gaattttttg attaagcctt gggtagtttt ataattctct ttttcaaaag aggtggatat
     3781 ttctggtggc aggccgtagc caagctcatt taaaaaattt atttcctctt ttataaaatg
     3841 ggaataatgg ttttgactta tcgatctgac tccattgacg acgatttggt aaagttcttt
     3901 ttgtttttga tcaaaaggaa gcagtttatt gacgatttct aaaaaataaa atagtaaatt
     3961 aatttgactt aaggaagagg tatctttgag gaaggccgtg actgattcag tttcggaaag
     4021 ccaataaaaa gaatttttat cgtaaagact gcttttgata atagtgccga tttccaggtg
     4081 accaaggcgc cgacttttta tagattggac ccctttagca agacagttta ttttgccgaa
     4141 atttgaggtt aataaggtga tgattaaatc accttcccga cgacgctgtt tgttgataac
     4201 cagagcttca gttgaaataa atttgggcat tagagtgaaa atttcagagt tttatcagat
     4261 tcccaggaaa atttttcagg agctccccca ttaacagaga tttcatattc aatcgctttg
     4321 gttcccggtt gtttttgaag gtaaagggtg taatttttac tcgcctttat gggagtggaa
     4381 tattgaagag aaactttgga gctggagttg gcatagagag ggtatttgtt gccataaaag
     4441 ccttcaaaga cttgcttgcc taattcttcg tatttgaggg cctctatttc tgaaccggtc
     4501 atttttatta gctgactacc aagggggaca tagaatctaa ataaattccg gtaggtagcc
     4561 gcatttaaac ataaatcccc tttttccaaa ttacagtttg aggctttggt cggattggtg
     4621 taagtgatag taattttatg ttcgatattg ccatctttaa cactaaggtc gtgagtaatt
     4681 ttttgagtaa taaaaatatt gcttttggca gaggcaaaat tagcgtcatt aagatggaaa
     4741 taatcagtgt tattatctgt ctgaatgata ttaccggcaa tattggcttt aatggccgcc
     4801 tgttgaatct cgggagaagt gaaataaaaa agaatgtgtt ttcgattaag attattaaaa
     4861 aaagcctggg ctaagggccc aattttgttt ttttctgagc ccatggcatt agccagtagt
     4921 gattgcatga gagggccaag aaagcctttt cggtcatttt caatgtaatt tcggggacga
     4981 ccagcaatcc actcgagatc ataaataatt ttagggcaac cattacagcg gggatcagga
     5041 tcgctggtaa aggtacccac ccgggtatct agttttccca atattttaac gatatcgaca
     5101 agaacctggg tgtcgagggc gataaccgcg tcaaattggg tttttttacc catggctttg
     5161 taatagctat ccagaaaaat ttgagcatca gtgggaaaat ccggagataa attgctatca
     5221 cgaagattta aataagggac attaatatgg taggacttaa taattcgggg agcgggagtc
     5281 gtactggaaa gcttgtcgtc gagatcgtat atattactgg aagctccggg aataattttg
     5341 ccattatcga ctttcagggt ggtataagcg gtccagaagc caccactggg acggagctct
     5401 ccatcatttt gaaaaatgac taggtaattt ctagctttgt ctttgccaag aagccaagag
     5461 acctgaccaa ggatgggttt gctgttgcca acatagtttt taatttctcc aatcatttcc
     5521 cggactttat tgaaatcagt atggagggaa atacctctga aattctccgg atagcgattg
     5581 atatcgattt gattgacgag ggtgtcaatt tcgttgactt ggctttcaat taaatcaaag
     5641 tgaggcaaga tactttcgat tgattgggtc aaaaaggcaa ttcggtcttc ggtggtttca
     5701 gcactactgg tagctgcccc tttcaaacct agaaaatcct gataaggctc gatagctttg
     5761 ataactattt ccccggtttc cagggcatca ccgccaattt taattaggtt ttgaccatca
     5821 tgataataat tactggcgac ggggataact tttagataag caagtttttt aaatttatta
     5881 tcaatggtgt ttagttcttc ccgcagggtt gaaacaattt ctttactttt ggataaatct
     5941 ttatcggaaa cagcgactga aagcgactcc gctaccgttt ttgttttttc aatttgagcc
     6001 agcaaacttt tgcccggtaa atagagaaat aaaaataaaa gtaagataac cgctaaaaaa
     6061 gcgctgagaa tgattaaaac agtccggttt attttaaatg attttttagg agtgagagtt
     6121 tttgtttttg gaggagtctc ttcttttttg attagcttgg ttggaatttc cataaataaa
     6181 gtttatcatg aaattaccag ggggaaatca tggctagagg agttttgaaa ataatgagaa
     6241 gctcttctgc caaggaacga ttgatgatat atttggcgtc taatctggcc cgtttttcaa
     6301 aagataattc gtttcttccg gagacttgcc aagggccagt gataccaggc ttggcactgc
     6361 gaataatctt aatatatttt ctggtatttg gatatttgtt ggcatattcc tttagttctt
     6421 catctcgata ggccctgggg ccaactaagc tcatctctcc ctttaggaca tttaaaaact
     6481 ggggcatttc atcaatggag gttgaccgga taaatttgcc aattaaggta actcggggat
     6541 cttctttagt ggaaagtttc caatcgttgg ctttatattt tttccaaagc ttcggataat
     6601 tatcttttaa aaatttatca ttatcaccaa tacgcatgga gcgaaattta tacatgtaaa
     6661 agagacagtt tttgccgact ctgagctgtt taaaaattat cggtccaggt gaagtaattt
     6721 taatgagaat ggcaatgata attaaaattg gtgagaaaat tataagtaaa aacagagaag
     6781 aaaaaatatc gatacctctt tttatcggtt caaataattt tacccgaagc gaataaaaag
     6841 gtaaggattt tctagtcatt tttgccggta aagaaatgtt ttaaattgag attgccaacg
     6901 attttggtga cgagatctcg gacatgaaaa ctgccatcac tggcaagatt gcaaattaat
     6961 agggcatcag gcgtgtcgac aatagcgagg tggttaacat cgacgaggcc aatcatcttt
     7021 tgagtttcag aaaccaggag acaatttttg gaatttactt cgacaaattt tatattttta
     7081 gggagaaggg caaaattatt cccacttttg ggcaattctt tataaattga tttccattca
     7141 ccaatatcat tccaattaaa ggaggcgggt attaaaacta atttttttga tttttcggaa
     7201 atagcgacgt caatcgcgag agagggagaa agagagtaaa ttttttgaat aatcttagga
     7261 cggtctaagt tgtcgagaag cttattataa agagggagat agtttttggc ataaagttta
     7321 aattctgcca gcaaagtatc gattttataa gtataaatac cactattcca aaaacagtta
     7381 ttctttttaa ttaatttttg ggc
//
LOCUS       DHGB01000057            4191 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_43177, whole genome shotgun sequence.
ACCESSION   DHGB01000057 DHGB01000000
VERSION     DHGB01000057.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_43177"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1685 a          592 c          777 g         1137 t
ORIGIN      
        1 catagatacg gagtacccga cagccaaagg agtggaaagt gccggcaaaa agatttttgg
       61 ctttatcatt atctaaaagt ttattcattc gctccttcat ttccccggca gctttattgg
      121 taaaagtaag taatagaact tctgaaggag aggcaagttt ttgctcaaca aaataagcgg
      181 cgcggtgggt caaaacccga gttttgccac taccggcgcc ggctaaaatt aatagtggag
      241 tcccctggta agttacagct tctttttgaa attggttaag actcccgaga atgttagaca
      301 taaaacaaaa aaattctaaa gactagagtt taaaaaatta aataaaaaaa ataatgctta
      361 tcaattatca attacgagtt attaattaac aacgagcaaa aattaatata atttaaggta
      421 gttgatagat tctggtttga cctgattcaa aagctagttt agctccaagt tctgcaaatt
      481 tttctgaatc aaggtcggga tatttttcgt attctttagc cccaacgata atgtatttca
      541 cctgatattt atccaaaatt gatttgactt cttcgggact gggagtatta aaattgcttt
      601 cattaatagt atttggaata tcgactggaa taatcaattt ctgatatggg tatattagtg
      661 atggattttc taaattattt aactgtgcta tttttttcca caaaaattca ttatttaatt
      721 ttaatttaga aattttccat aaactatcgc ctggattgac tatgtattct tctggagatt
      781 gggtattatt ttttatttca gaatttttag aaacaaaatt atagatttta tcaacctcag
      841 cctgacgggc ggcgggggcg tcgtagccac ctcgccagag ccattcatga accacccagc
      901 cctcaatggt gggtaaaccg gtagccatag aaaattgatt atatgtcgta tagctatctc
      961 caacggcctc tactatatgg ggctggccgg aaacattgag atttaaccaa ttgatagcag
     1021 caagattatc tggataggaa atttctaaaa attttaaacc ccaaagacct ttatattctt
     1081 ttaaaccata atatgatttg gtcgcaaaat aggaatagct gaggtgaaaa ataaaaacta
     1141 aacaaaaaag aaaacgatat ataaaaatat ggcgaagccg aataaagata taaccagcaa
     1201 tcagggaata catgataaag gcttggtaaa ctaatttaaa catggtgttg gcccgacgat
     1261 aatcaaaaac ataaatgtct ttgatataaa aaatttcagg aattattact agggcagtag
     1321 cagtaatgaa aatagctaaa acaaaataat cggtaggcaa aagattgctt cgtcgctttg
     1381 ctcctcgcaa tgacgaaata ataaaataaa aaacaaaagg tagagagatt agccaaaaac
     1441 cgccatataa aacaaaaagc tgataaaagg gtgagcgagc atcagaaagt tgtaaacctt
     1501 cgaccatggg agtgaaatta agagaaaaag ggagagtaaa taagtaccaa aaagcgatag
     1561 tcaaaagacc ggtaaaaatg gtcggccaaa attttttagt aactaaaaaa gaagcgatgg
     1621 caaaaagtaa accatagacg gcaaaatccc aggcattggt catataggcg attgagagca
     1681 ggagaccggt gggagcggct agtaaataag attttttatt atttgaaagt gaggttaggg
     1741 aaataaaaag aaaagccata aagaaaatta ccagagggat atcattcata tgaccgtgaa
     1801 gatcagccac cacaaaacta tacatgggaa attcgtgaat agttttgtcg gtggtatcag
     1861 ggtcaaagcc aatgaaacga gtagcatcgg ggtaccaata agacgcagat gcctctttta
     1921 tattttgttg attttcaatc atatttttgc caattttata gacaaagtga aggttgccac
     1981 caaaagtcag gaggaaggcg gaaagaagac caccaacaaa atacaaccta aatatgggtt
     2041 tttttagagt gctgtaaatt aaatttgagg tcaaggaaaa gccagaaaca aaggtcagag
     2101 cgcagacagt agcaatagat aagttgtatg tgatggctga ggaaatacca gaaaatttgg
     2161 ttaaaaaggc aaaaactaaa tgaccaaaat aataataatt tattgactgg cctgcatacc
     2221 acatatcggc tggaggaaaa gactgactac gaagaactga attgacaaaa ccccagtcca
     2281 taaatttttc aagaccctca atatcaggag caaagccacg gacataggac caaagcgtta
     2341 agataacaaa aaataaaatt tcttcgaaaa gaaaaattcg ccaatatttt ttaataagac
     2401 ccttaaaata gactttattc ttaataatta aataaaaatt aaaagcagca aaaacagctg
     2461 taacaaaata aatacagggc aaagtaaaag tagtaatctt aattatcccc aaaatcaaaa
     2521 gcaaataact taagacaaaa agagaacaag ttttggcgaa gatgtaacct ttatcccaaa
     2581 aatttttaaa aagtaaaaaa gttaaaggta aagagaaaga ggaaagaaaa aagaggctta
     2641 gccaccaagt aaaaatgtat ttaaaatcag aaagcatttt ttagattaat taatagaata
     2701 atagattaat agattattag ttgagaaaaa aaatagtatt aaaaattatt tttatataag
     2761 aatcaattgg agtatctatt tttaaagaag aaattaacaa ccagattaat ataccaataa
     2821 ttattccacc aataatatcg acagggtaat gaaaaccgac cagaacccta aaccacaata
     2881 aaggaataac aattaaaaaa aataaccaca aaggaaatat aggaaaagag ataaaaaaca
     2941 aaagaaaaat agtggctttg atggtgtgac cggaggggaa agaaccagtg caataccact
     3001 ttttaactaa accggcaggg atagaatcgt gacgaaaaaa cattggccga caccaaaaat
     3061 tttttttctt aacaaatctt tcaactattt cggcaagtaa tgtggccaaa aataataacc
     3121 aaaaaaatga aaaattatgt ttgattaaaa gaaaagaaag atagaaaaaa aataaccaaa
     3181 tgacatagtt taaaaaaact aaaataatcc gaccaaattt aaatgaagat aaaagacgta
     3241 aataatcagt ctttggctgc caaaaaagaa gagctggatg accagtagac ataatgagat
     3301 aattgtatca attaaatgaa gagaatttta attggagtta cctattatca tccttatatt
     3361 tccggagtga gtgtttatgc caaaatttta gcagagaagc ttatgaagac aaacagagta
     3421 gagataatta gcgcgaagta caaaaaagga ttaagaagtg aggaattagt aaataaagta
     3481 aaaatcaaca gagtttctgg ttgccaaatt ggaaaaggat tcatgatgtg gcaataccca
     3541 ttagtaagtt ttagaaaagt tagaagtgtc gatattgtta actgtcattt gccatcaata
     3601 gaatcttttt ggctcgcact atgggcaaaa atgttaaaga aagaattgat agttacctat
     3661 cattgccgtt ttgatagtgg aaataaattg ttaaatttta tgattgaatt aattcactta
     3721 cctgccttga tttgggcaga aaaaattgtg gttaattctc ttgattatgc aaagggaaat
     3781 aatttgctaa ttaattatca agataaactg gttgaaattt tccctccgat taaaattgta
     3841 aagagtcatg aaaaaataaa aattaaaggc aattttaagg tgggatattt gggaagaatc
     3901 tcgaaagaaa aaaaccttga gtgcttaatt agatcatttg caaaattgcc aaaagaatat
     3961 gtattagttt tggcgggacc agagaaaatc gtaggggaaa taaaatacca aaaattaata
     4021 aataatttaa taaagaaaaa taaaaatatt ataagaatgg gtgaaattga taatccggta
     4081 aaattattta atagcattga ttgtcttgtc ttgccgagca ataatccctt ggaatctttt
     4141 ggaatggtgg cggcagaagc aataaaaagt ggttgtccgg taattacctc t
//
LOCUS       DHGB01000058            5445 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_58659, whole genome shotgun sequence.
ACCESSION   DHGB01000058 DHGB01000000
VERSION     DHGB01000058.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_58659"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1546 a         1117 c          889 g         1893 t
ORIGIN      
        1 tgtcagtttt tacggtcaat tcaagatata tttttctccc cgacattagc tccagttctt
       61 tcctggcatt atagccaatt tcttttattt tttgcccatt ctttccaata atcatttttt
      121 tgtaacggtc ggcactggta taaatagtcg cggtaatcac aattaacttt ttcttatctt
      181 caattttatc gattcccaca aaaatagtgt agggaacttc ttctctcaaa aataaatagg
      241 ctttctcccg aataatttcc tctatatatt ccttagagct catacttatt attggcttgt
      301 tttcttgctg gtaaatttca atttctttta ttagttcttt atcatttttt tccggaagtt
      361 cgtcaaaaat catcttgatt agccctttaa catttttttc ttttagagcc gagacagaga
      421 tacttatatc aaattcgtct tcaaggtaat tatagtcagc caaatgatct ttactcccca
      481 cagcggcatc aattttgtta taaaccaaaa tttttttcgc tttgactttt cgcagtagcc
      541 caatcacttt attttcttcc tcgttttttg gccgagaaat atcgacgaga cacatgatta
      601 tttgagcttt gtttaatgtt ttgggaacct cctgattaac ttttttaccc attaaatcag
      661 ttacttttcc caatactcct ggagtatcgg taaataaaat tttacctctt tcatcttcat
      721 aaattgccaa aatatttttt ctggtggttt ggggtagatg ggaagttatc gccacctttt
      781 gctgcatgat cgcattgaga agggtcgatt ttcccgtgtt tggcctaccg acaatcacca
      841 cccggccgta ttttttcttt ataatttcgt tcacgtctat attttaccct aaaacactga
      901 attattcttt aataaaaata tcaatatttt tccactttac acttagcagt aaaaatgata
      961 gactgctaat tatgccagat ttaaagccga tcatcaccaa aaaagtttta cccctcattc
     1021 ctcttcggaa tgtggtttta tttccttcgg tcgaaacctc attatttttt ggtcgaaagg
     1081 aatccagtaa ttcactactt gaagcttatg ataagtatga ccgattagtt ttaattactg
     1141 ctcaaaaaaa tgccaatatt gaaaagccga ccatcaatga tatttataat gtcggtgtta
     1201 tcgccaaaat tgaacatatt ttacaaactg acggctctct gcacgccatt gtcaagggac
     1261 tttctcggat tagtatcgtt aactttgttc agactgagcc ctttattctg gctcagtatc
     1321 aggaattacc tgttatcaac gagccggttg ccgagattca aaaagctgct gaggctattt
     1381 tatctcaatt aaaaaaggcc ttttctctcg gccgtcaatt tgatctccct gccatgatgc
     1441 agctgagtac tggggtttcg tcctccgatc tggccgatca agtctccttt tccatcaata
     1501 ctaaattacc cgaaaaacaa caattgctcg aaaccctttt agtctcaaag cgtctcgaac
     1561 acgctaccga gctattaatt caagagatga aagtggctga tttggagaga agtattgaag
     1621 aaaaaactca ggaaaaattt aactcagcta tcaaaagaaa tattcttgaa gagcgtaaac
     1681 atcaaattga acaggaactc aaaaaaatga gtaaggggag tggtggtgaa actgatgagc
     1741 tggaaaagaa actcaaaaaa gccaaaatgc ccagagatat caagaaaaaa gttctcaaag
     1801 aatacaatcg tctcatagaa atgggatcta tggctccgga ggcctcttat attagaactt
     1861 atctggaaaa tgttattgag ctgccctggg gtaaatatga cgaaattgat atttctttaa
     1921 agaaagcgga ggccatctta aatcgtgatc attatggcct taaagatatc aaggaaagaa
     1981 ttctcgaata tttagctgtt attaaattaa aaaatgaaca gcatgataaa gataccaaaa
     2041 ccaccaatat tctttgtttt attggccctc ccggtgttgg aaaaacctct ctcgggaaat
     2101 ccatcgcctc agcccttggc cgcaaatttg ttagggcctc tctcggtggg attcgtgatg
     2161 aagccgaaat tcgcggtcat cgccgaactt acgtcggggc catgccgggt cgtattgtca
     2221 gtggtattaa agatgctaaa tcaatgaatc ccgtttttat gcttgacgag gttgataaaa
     2281 ttggctctga ttatcgtggc gacccttcgg ctgctctttt ggaagcctta gatccggagc
     2341 aaaacaataa ttttaccgac cactatttgg attttcccct tgatctttcg aaagtatttt
     2401 ttattttgac cggtaacgat tattccacta ttccttatgc tctccgggac cgtttagaaa
     2461 ttatttcttt tcctggttat actcaggatg aaaaatttca tattgctgcc aaatatctag
     2521 ttagtaaaga attaaaatca aacggtctta aagaatctca ggtcactaaa tttgatgatt
     2581 cgacactcaa atatctcgtt gataaatata cccgagaagc cggagttaga aatctggaaa
     2641 gagttattgc cgctatttac cgtaaatcag ccaaaataat agtttcaggt aaatcaaaaa
     2701 aagtaaatat caatgataaa aagcttctcc aaaagttttt aggtcctgaa aaatttactt
     2761 cccagcttaa gggtaaaaaa gacgaagttg gtgtttctac cggtctggcc tggacctcgg
     2821 ttggcggcga tatcttattt atcgaagtca atattatgcc aggaaaagga cagttaattt
     2881 tgaccggaaa actaggctca gtcatgaaag aatcttgtca ggcggctctt tcctttgttc
     2941 gctctcaatc aaaaaagtgg ggtataaaac aggattttca taaaattgat attcacatcc
     3001 atgtccccga gggagctact cccaaggacg gtccgagcgc tggcagtgcc attaccactg
     3061 ccttagtttc agcgttaacg ggtaaggcta tttcccacga tattggcatg actggtgaaa
     3121 ttactcttcg cggtaatatt ttagaaattg gtggactaaa agaaaaatca ctggcggcgc
     3181 atcgagcggg actaaaaact gtttttatcc ctgcggataa taaaaaatct ttagttgata
     3241 ttcccaagga agtcaaaaaa gatattaaat ttatccccgt ctcccactat tccgaaatct
     3301 acaaaaagat tttttgataa cgtcattgcg agcgatacca cgtcattgcg agcgtagcga
     3361 agcaatcttt acagtatttg actatacatg tctttccagt ctggattaaa tttgtttatt
     3421 aaatctagtt tcttctttct tgaatcagcc ttaatttgtt tttctctttg aattgccact
     3481 gttatatctt caaaaaattc ataataaact aattttgtaa catcatattt ttttgtaaaa
     3541 cattcattta gcttttccct atgttcccaa actcttcgaa ttaaattgtt agtcactcct
     3601 gtatataaga cccgattatt gtagttagct aaaatataaa cggacgaata ttgtctcacc
     3661 cattaagatt gccacgtcgc tacgctcctc gcaatgacgt atttttacat tttttctacc
     3721 gttttgattc ctaaaagatc aagcccgatc ttaagaattt ttgctgttct ttcggttaaa
     3781 aacaatctct gttcttcttc attttcgcca ataattctaa atttggcata aaattcattg
     3841 tattttctcg ccaatcctat taaatattct gctaccactg ccggagaaaa tctttccgcg
     3901 gcctctataa ttttttcttc aaatttataa aactctcgca gtagtgccat ttcctcatcc
     3961 tcagaataat taaattctgt atttttaatt gaagattgaa gattgaaaat tgaagattta
     4021 ttcaaaacgc tcaggcatcg agcataagta tattggaggt atggcccgga atttccctcc
     4081 atactcatca cttttttcca gtcaaagata atatcttttt tcggatcttg cttcaaatca
     4141 ttaaatttta tggcgccaat agctactgct tttatttttc cttcatcatt attgggagca
     4201 atttttctgg cttctgacat cgctctttcg attacctccg acaagtggat tgtctgacct
     4261 tttcttgttg aaaatttacc ctcggtccat ctaatcatgc cgtgggcaac attataaaat
     4321 tgcggtgtcc atccgagcat ttttgaagtt tcgaatattt gccggcaatg tagttcttgg
     4381 tcggccccaa tttcataaat aatcaaatct ggattccaag tctccattct atatttaact
     4441 gtggccatat ctcttaaaaa ataagtagta gttccgttgg atttaataac catggctggt
     4501 ggcattctct taaattccac tatttttgct ccttcacttt cttttaatat tcccttatct
     4561 tccattaatt taatcactgc cggcattttg tccaaataga atgattcacc gtaggcataa
     4621 tcaattttta ctccgagaag ctcataaatt cggtcaaatt cgttcagtga aatatcaata
     4681 catttttgcc aaatttctgt ggcttcttta tctttttctt ctaattttga aaaccactct
     4741 ctggcctcgt cctccaatgt agtctctttt tctgcttctt cgtgaaattt tacgtaaaga
     4801 ttttctaagt cttcaatgct catttcctca actgatttac tgccccactt ttttatggct
     4861 acaatcagtt ttccaaattg ggttccccag tcgccgaggt ggttatcgcc aagacatttc
     4921 catcccaaga actgataaat attataaata gcttgaccaa tattggtcga tcgcaggtgt
     4981 ccgattccaa aagtcttggc aatgttggga gcactgtaat cgataaccat tgttttcccc
     5041 tttcctacat tttcaatttc cttttgccat tccccgcctc caattttttc ggcttcatta
     5101 attagtttat ttttatttat aaaaatattg gtaaatcccg cgatcttttc aattttttca
     5161 attaaatcct tattctctct gattttaact tctcctctga tggcaaaatc tccaaatttt
     5221 tcgactgtcg gatgatcaat tttttcttca aagttatctc ccagtagttt ttttagctca
     5281 tccttaatca tgcctaattt tagcagattt ttaaaatact ttagtcccag ctggcaaatc
     5341 tttttccaga attaaaatac ttagttgctc cttttggctc gccgtagcgt cggcggaggc
     5401 ggcaaatatc attgcctcag aatcttcccc cataattttt ttggg
//
LOCUS       DHGB01000059            3025 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_179381, whole genome shotgun sequence.
ACCESSION   DHGB01000059 DHGB01000000
VERSION     DHGB01000059.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_179381"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT          849 a          627 c          557 g          992 t
ORIGIN      
        1 cccaggcttt aacgattact actcttggtt ctctagccga gcagcaatat cttcaaagta
       61 gtaatggtga aatttctaaa cgttatatcc attattattc cgccgctcct ttttctaccg
      121 gccaaactgg tcgttttggt cgtcctggac gacgcgaagt tggtcacggc gctctcgccg
      181 aaaaagcctt agttccggtt attccttcaa ttgaagaatt tccttacacc attaccttaa
      241 cctccgaagt gatgtcccaa aatggttctt cttccatggc ttctacctgt ggttccactc
      301 tctccctcat ggatgctggt gtccccatta aagataaagt tgccggcatt tctatcggta
      361 tgatttctga tgataagggt aaatatgtat tattgactga tattgctggc atcgaagacc
      421 actgtggtga catggatttc aaaatcaccg gtactcggac tggtattacc gccattcagc
      481 ttgatattaa gaggcttggc ctctctcttc aaatgattac tgatactttt gttgcttcta
      541 ccaaggcccg ccttcaaatt ttggacaaaa ttgatacagt tttgccagcc cctcgggccc
      601 agctgtctca atatgctccc aaaattaaga tggttaccct ccccgaagat aaaattggtg
      661 aagtcattgg ctctggaggt aaaactatca aagctttaat ggaaaaatat ggagtcgaaa
      721 ttgatattga tgatgatggt cgtgcttcaa ttagctcagt agataaggat aaaatcgaag
      781 cctgtgccta tgaaattgaa tcaatgatta aagaagttca agttggtgaa gaatatgacg
      841 gtattgtcac tcgagttgag gcttatggcg ccttcgttga atttcttcct ggccgagaag
      901 ccttactcca cgtttcagaa ttaagtgggg gattcatttc gaatcctgct tcgatcatta
      961 aaattggcga taaacttaaa gtaaaaattt ctggttttaa cgataatcac cagattaaat
     1021 taagctcacc tgagtttaag gccgctcatc caggtaatgc caattccatg ccggagcgtc
     1081 agggtcctgc agcttcaggt cgtcctcctt tccgcaattt cggtagccca agtggtcaaa
     1141 accgcgggag aagataactc aaaattttta atctaacccc tccggtccgg cgttcaccgg
     1201 accacctccc cttgacaggc gaggcgagaa caagccatta atattttttg cctcactcca
     1261 tttagatcga ataatacttc ttgattggta aatctaatag tttgtattcc tatttgggat
     1321 aaaaacttat ctcgtaaaat atcgttttcc ctctttttaa tgtgtgatcc tccatctatt
     1381 tcgattgcta aatttatttt agaacaataa aaatcagcaa taaacctatt tatcggtttt
     1441 tgtctgctaa acttatatcc tgtctttttg tatctcaaaa tttgttgcca aataatttct
     1501 tctgcctccg ttggattatg tctatttttc ttagccatct gagttaaatc agaaaaataa
     1561 ttaatatttc cacgtaccgg gtttaaagca tcaatttcta ccttcttttt cataaaataa
     1621 gtatttgttt ttcagcctcg cctgtcaagg ggaggtcctc gatttaataa tcgaggggag
     1681 gggttagatc aaaaataatg tatacttttt atgtgaattc gacatttaca gctgctgatg
     1741 ctattgccaa attaaaagcc aaggttggtg gcgttaatct gcctcccggt ttagaggaaa
     1801 aaattaatga atatttttct cagcttgagg taaattctcg ctctgaagat actttttggt
     1861 cttattatca gtcaatttct aaatatttag attgggtcat ttctttgcct tggaataccc
     1921 gatctcagga tattctcgat atccgatatg ccaaagaaaa acttgatgaa aatcattacg
     1981 gtctgagtga agttaaggag cgaattttag aatacatttc cgttttagct ctccaaaaaa
     2041 atcgttctcc tgatattgtg cctcgggctc ctattttgct ttttgttggt ttagttggta
     2101 ctggcaaaac aactatggcc aaatctattg ctgatgttat gggaagaaaa ttaattcgta
     2161 ttcctttcgg aggtctgggt gaccctcttt atcttcgtgg ccaatctcgg cttcatcccg
     2221 aggccgaacc gggtgctatt attcgaggtc tggctcaatg tcaaactaaa aatccggtga
     2281 ttcttctcga tgaaattgac cgggtggccg atgattccct caacactatc atgggtattt
     2341 tagttgaact tctagatcct gagcaaaatg cccgctttac cgatcattat cttgattatc
     2401 cctttgatct ttccgaggct ttttttgtgg ccacttgcaa caacacttct cgtattgcca
     2461 ccgctgtgat ggaccgtctt gaaattattc aaatgccttc ttataccgat gaagaaaaaa
     2521 ttgccatagg tagggattat ctcctgcctc aagctttaaa agaaaatggc ttaaccggtg
     2581 aagaactgaa aattgaccaa aatatttggt taaacatcgt tcgccctctt ggttttgatg
     2641 ccggtgttcg gactctcaaa agaaatatcg aaggactttg tcgcaaaagt gccgccttaa
     2701 ttttatcggg aacaaagcaa ataattattg acgaaactaa tattaaaaaa tttatccctc
     2761 aatggtaaaa aatatgaaac aaaaaaaagt cagttatagt aaaatttttc tttttccact
     2821 ttctttagcc attatcttct tctcctctat catcattatc ttttccccag tcggtaaaaa
     2881 aaatattatc aaaatcactc caacaatcac tccgaccccc gcaattttaa aataatttaa
     2941 cttcatcaaa taatgccttt aactaaagat caacaacttg agtatattgc ccatttggtg
     3001 ggggaatgta aaaaatgcga tttat
//
LOCUS       DHGB01000060            4466 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_30894, whole genome shotgun sequence.
ACCESSION   DHGB01000060 DHGB01000000
VERSION     DHGB01000060.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_30894"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1167 a         1017 c          694 g         1578 t
ORIGIN      
        1 accctaatca aagctctcta ttttaatttt gctatcatag ctcatgcgta tcgctctagt
       61 tcaggattat ctcaaggaat atggtggcgc cgaagcggtt tttgagactt taactgacat
      121 tttccctgag gctgatattt ttacttctct ttattgccct aaattttttg gtccccaccg
      181 acagcgttta gaaaaaaaat ggagaaatag gattcaccaa tcatttttca aacatattcc
      241 ctttgcttat aaaataattt cccccnnatt atcacctccg ctaccggtgc ttatttcccc
      301 aatgctctta ggaaaaaatc agccaagcta atttgttatt gtcacacccc cccccgctac
      361 ctctatggtc ttcctacggc ccgtgattta aaaaaatatc ctatttttaa ctttttagct
      421 caaattgcca atcattttct tcgtctttta gactttaaat atgcccagaa tgtcgatctt
      481 tttgttgcca attcgcagac caccgccgcc cgcatccaaa aattctacca tcgggactct
      541 gtcgtcatca accctccaat cgacttaccc gatgaaaaag tgaagcgtga attttttccg
      601 cgcgcaatca tccgaggtaa tccgaagggc gtgagcacgg taaaaaattt gcgcgaacaa
      661 tatttcttaa cgggtggtcg cttggctcgg gccaaacgct acgatattgc cattgaggct
      721 tgtaatcaat taaaagctcc tctcaaagtt tttggtcgtg actttgctgg ttttgaatca
      781 gaattgcgaa agatctcagg ccctactgtt gaatttttgg gtgaagttac ccaggaggaa
      841 aaggctaaat tgttttcaga ggcctcagct tatctttttt gttccgataa cgaagatttt
      901 ggcatggttt ccgtcgaggc ccaatcttat ggctgccctg tcgttggtta caagagcggg
      961 ggaattatgg agacagtaat agacggtaaa accggaattt tatttgataa tctaactccc
     1021 gaatcttgtg ctcaggctat tagtaaagta ggagctttgc ttgccaaagc ccaaactaaa
     1081 aacgcttgta tagctaacgc ctcccgattt tcccgcgaga attttgttaa aaaaattaaa
     1141 tccctcgttt ctactactca tctactcaac taattatctg ttctttaacc atctctccgc
     1201 atcaattgcc gctctggctc cggttcccgc ggcaattatc gcttgtcggt gtctttcatc
     1261 catacagtca ccagccgcaa aaattccctc cacagaagtc attgagggca gttcttcatt
     1321 gctccccacc ttaatatagc cactttcttt aagttcgatt aaatctttga taaattcagt
     1381 ttttggaatt ctaccaatag cgataaataa tccatctata ggtaatattt tttcctcact
     1441 cgttttaaca tttttaattt taatattctg aagactggaa attagaggtt gaagattggc
     1501 attaatttcc accactacac tctcccatac catttcgatt tttttattct ccagtgttct
     1561 tttagcttca attggagtgg ctcttagttc atcccggcga tgaattagat agaccttact
     1621 acaaatcttt gacaaaaaat tggcttcttc acaagctgca tttccgcctc ctactactgc
     1681 caccaccttg tctctaaaca tcatcccgtc gcaggtcgca cagcctgaaa ttccatgtcc
     1741 gattagttct ttttctcctg gtagttctag ccatttagcc gccgctccag tcgcaataat
     1801 tactgcttta gtttttaaaa tcttgccgga acttaattct atttgaaaat tggttaatgt
     1861 ttcttgatta ttgtttggaa tacttggatt ttggttgttg attattttca aaacggtatc
     1921 gttttgaatt tccacacccc agttttttac ctgttctagg gtatccatca tcagtttaat
     1981 tcctccaact cctttgggaa aaccaggcca gttttcgacc atcgttgttg ttgtcagttg
     2041 acctccgggc atatttcccg caattaccat tgtttttagc tctgctcggg cattataaat
     2101 tgcggctgtc agccctccgg gacccgaacc aattataatc gtatcgtata tttgattatt
     2161 ttctgtcata cctcatttta ccctaattta ttttttgttt taggctttag actttttcta
     2221 ctcattgata attgataatt gaaaattgat aattgtttaa tgtctcgcga aactcgcaaa
     2281 gtttttgcct ttgttttaaa aattatttcc gatcgcaccg gtctcttttt ttgggccttc
     2341 gttcgttttc tttccgccat ccttcctctc attactatct atctctattc tcttgtcatc
     2401 aaacaattag aatcaaaact ttcattgtcc acaattatca tcactcttgt actgaccttt
     2461 attgtccgtc ttttagacaa ttattttcgt cttctttcaa ttactcgact agagtacatt
     2521 attagcaaca tcggttttga tattcataat ttttttctgg ttgatttaaa atcagaatca
     2581 aaggaagaac gtgccgccac cattcaagct atccgtaatt tttccgatgc ctccagcgtt
     2641 actctaaatc tcattaaaca gcctggaatt gacagtcttg tttcctttct ttttattccg
     2701 gttattttat ttatgattga ttttccttct tttattatta ccttagctta tatcttaatc
     2761 tattttttca ttgacactta taccactcag cgctatagcc agctcaaaga tattcaaaac
     2821 tcaaaaaccg aagcttacta cgccaagctt caagagagta atgattatga tcttgagcaa
     2881 aaatcttata cccgtcattt taatcgtctt tgtaaatggg gatttaccga atggtctttt
     2941 cttcaaaatt cttctgtttt tttctattgt ctaaatttat tatatttaat ttattccgta
     3001 tatactggtg aaaaaagtat ctctggctta gtcttagtta tgggttatgt gactcaaact
     3061 caaacttttt taaattctct ttccaatatc aaagattctt tcactgacat gaacgtcggt
     3121 ctcgagcatt tagccaaaaa cgaagccatt tccaccattg acctcgacga cttaatttaa
     3181 aattgagtta aaatatatcc tgtgcccgaa ctacccgaag ttgaagttgt caaattgcat
     3241 ctcgagcaaa aacttcttca taaaactatt accaaaattg aaattttaac cccaaaatca
     3301 tttgtcgggg atcctaagct tgtcgaaggc caaaaaatag taaaattttc ccgccttggt
     3361 aaacaacttt ctatctattt atctaatcat ctaattctcc tcgtccatct caaaatgacc
     3421 ggtcaattaa tttacgatgg ttttatgctt ggtcatccaa cccccgctat taatccaaaa
     3481 actactcgcc ttatttttca ttttagtaac acgtcattgc gagaaggtac tccgacgaag
     3541 caatctctcc tttatttcaa cgatcaacgt aaatttggct ggatccgggt ctttagtcct
     3601 aaagaactaa agctctatca atctgaccta ggtcttgatc tttttgatcc cgacttttct
     3661 cccaaatatc tctattctca gcttcaaaaa tcccgtcgtc cgattaaaat aattctttta
     3721 gatcagcatt attttgccgg cattggcaat atttatgcct gtgatgcgct ctttttggcc
     3781 ggtattcatc cgggaacgcc ttcaaacaaa atctcccgct ctaaagccct aaagctccac
     3841 caatctttac tctccatcat gcggcaatca gttttggccg gtggctctac cgctcgtgat
     3901 aataaatatc ttcgtcccga tggtaccaaa ggccaaaatc aatttttctt tcgggtttat
     3961 cagcgaaagg gcgagccttg tcttaactgt ggcaccaaaa tcaaatatct aaaaatcggt
     4021 agccgcggca ctttnnnnnn nnattttttt taattatttc accaatccca tcggcatata
     4081 ccaactaaag gcttttttac aaaccggaca ggtcatcggt ggttgttttc caaagtggat
     4141 atagccacag ttgacacatt tccactccag ttctgtctca ctatcataaa gtgttttttc
     4201 atccatcttt cgggcgataa tgatatatct ttcctcgtgt aatttttcca cctcttttag
     4261 ttttccaaag agtctaatag cttctgtttc attttcttca atggctgttt tttcaaaatc
     4321 tggatacatg gttgtccatt catatttttc tccctcagct gccattttca aattttctgg
     4381 agtacttttg gaaaaaggac caatttcaag atttcccaca atttttattt tatcggtgat
     4441 aagttcaaac tcttcctcgg cgtgaa
//
LOCUS       DHGB01000061            6610 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_63430, whole genome shotgun sequence.
ACCESSION   DHGB01000061 DHGB01000000
VERSION     DHGB01000061.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_63430"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
     assembly_gap    5906..5918
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2036 a         1195 c         1034 g         2330 t
ORIGIN      
        1 ttaaaatgtt gctttttcta aatattttca acttcttaga tacaacatat taactacata
       61 taaaatacat ataaacaaat tgcaagttat cattaaattc gtggtagaac caataaaatt
      121 aaaatcgata cccaacacta aatttaacgc catgtagcag aaaatgttac tgatactcta
      181 ccggattaaa ggggattaaa gctgtgtatc ctaattctgg ttcaatattt taaaaaaaat
      241 gtgtcagcta tttttaaatt taatacaatg cccaaggcat acccaattcc cgactgattg
      301 tcgatgccag tttcattttt attgcttatc tctgtatcaa ttaaaaaacc agttctcagg
      361 taaaaccgct ttagaaagtc aattttaact tttagtggaa ttcttagcat tctggtttcg
      421 atattttcaa ctgtggaggt cggtagtccg gttggtgggg gattataatc attaagagtt
      481 ctttgaaaga tgattcctga atttaaccag atgtttgtgg aaacttttct attatagttg
      541 atacctaaac aaaaaccttt accgttttcc cacgaccccc aaccatccat cttaacaaat
      601 tgtataacac tattttccaa ataacctgca ataatatcaa tttgattttc ttttgtgtca
      661 gcttgcgaaa atgacaggta gctgacaaaa cacagggata gggctatttt taaatctttt
      721 catagtaaat ttattttaag gtttaactta ctgactaatg ttcccactta attctcatgg
      781 attatgatgt taatttccaa acacatacaa tcctcgtgat ctttgtgaat ccctttgaga
      841 cctttgtgtt aaaaatctca atcgttgaat ataaccacaa agaacacgaa gtaagcacaa
      901 agtttcacaa agtatttaat aagaattgga gttatcaata gtttgataat gaattacatg
      961 taatttcaat ccatttgtaa atgtttgatt tatagaataa tatttatacg tgcgaaacat
     1021 tagtaaagtt ccatttctct tttttttgag aattcccaaa aaacaaatcg tctaattcgt
     1081 ctgtttaaat ttatacaagt tattactttt catattttat ttttttaacc gcaaannagg
     1141 tcgcagagtt ttttctttgc gcactttgcg taaaaacttg gcgttctttg cggtttttta
     1201 tcatcagttt atatttttat tatttcaatt ttttacacaa aaggtaatat tgaatcgtta
     1261 taatcatgcc cgagtgcgtc taaaatccgt catggatgtt tcaaattgca ttccttgccc
     1321 tgtgaaataa ttttgccttt gggtaattcc tgttcactgt gaaatatcag cattcgttgg
     1381 attttacctt aaaaaatggt agtatttcac agggtgaaca ttgagacatt gtccctaaca
     1441 ttcctctacc ttcctatacc ctcctctttt ttatattaac ttccagttat ttttcttagg
     1501 gctatattac ccgataatga gggactatca agcactggaa acaacgtttt cgtcatcggc
     1561 gttactaccg aaggacaagt cgtaagcccc ttacgtgtat gctgtgacca gtagttttca
     1621 atttactact gccttttttc ttttacttgt atggtagcat ataaaattgt ccacaaaatt
     1681 atgaacaatg aaataatcaa aaagctgtta aagtaacttg tataggtaag tagaatcccg
     1741 ataattattg ggcccaaagc ctgtcctgta tcagcaatgg taccaaacac gccaaggctg
     1801 gctccaagtc gtttttctga tgccacttcg gctacatagg caacagttga ggtagttaca
     1861 attgcctcag caaaaccaaa aaatactgat attattgtca gcagataaaa ctcagaagtt
     1921 aatgtgaacc ctacaaatga aataccacaa agaaacatac ccagtgaaat aagcttccac
     1981 cgttccattt tatctgagag ataacccata acaggtttta acacaactga agtacccgtt
     2041 acaaccgacc ataagattcc agcctgaagc actgtaagat ggtattctgt cactgctaat
     2101 attggtaaaa acgccattgc tgcacccatg gtcatatttt gaatcgattc cattgaggaa
     2161 gtcattaata ttttaagatt cctgcctgtg tctcttaatc catcaatgat tttgctaaaa
     2221 gaatctttta ttcctctatg ttcggatgac ggttctactt taggaagcac aaagaaggct
     2281 actattaacg caagtactcc taaaatcccg caaaaaaggt aagcgtaacg aaaatcgctt
     2341 atagtgtaat cctgtccctt tgccagatag tataatagcc agccaccaat taggctgcct
     2401 atagcattag tcccaatttt tatcaatgag aaaattgaca ggtatttccc cttttcttta
     2461 ccagcaatat tggcaacaac cgcatttgct accggcccgt atattgaggt agccattccg
     2521 tgaataattc ttattacaat aagcatccag gccgcagtta caacgatata gctaaagggc
     2581 ataacggcaa aaatgagtgc cccaaaaaga agcattttcc ttctgccaat gtgatcggat
     2641 aaggctcctg aaggggcttt caggaaaatg cctgttacag tggatgcccc cactattata
     2701 ccgattagcg atggagatgc tcccaaaaac aatgcaaaaa gtggcaacaa tggcgttctt
     2761 gcaatgttat agctcaacct tgcagaaaaa tctactaatg agatattaac gagtgaacgc
     2821 catttgctca tgattacttc gcttggttct ctatctgttt tctgaaataa gcatataatg
     2881 catcgtaaag atagaactct ttttccagag tttcatagtc atctttgcac aacaacgtat
     2941 agcccctacc aagtacatca agtgcaacag caagtggtga aggattttcg gtatgtgtat
     3001 ctgcttctcg gacaattagt tggatttttt ccaacgcagg atcctgaatg ttatacttct
     3061 ccataaaagc atcaaacgag catttattac catgatgccc gaattctacc cctttcatat
     3121 cgaaggggat gccctctttt atggttgatg ggtctgtatc gctggggaca aagatgaact
     3181 cagcctcacg gtcaatgaat ctcctgataa gccaggggca tgcaactctg tccacatgaa
     3241 ccctttctct ggttacccac ttcatgatgt acctccttaa gattgttttt catattacag
     3301 ggtttcgctt aactgttggg gggttgggct gctggggggt taaaaaccag cggcggtatc
     3361 ctacgttaaa aacatactaa catactaaaa actttggtat ctgcatcatc aactccagaa
     3421 gatttaacca catttggggc tgtgttttat atttttgtca tcaaaaattc taatgctttc
     3481 ttgttgtata tgaaagtgat attttttggc aaaatgccag tttccttata tttctcttca
     3541 gtttgtttct taataaagtt aggcgttgca aattctggat tattcctaat atcatattct
     3601 ttcattaaaa caaaaagttt gttctttttt acgtcaggtt tatttttttt aatttctaag
     3661 tataattcat taaaactgta tgggtattta tcaataggtc tttgatactt taaagcaatc
     3721 aagtaatctt cattttgttc gctgctcaaa gacgataaaa tatccgcttt gtttgggttc
     3781 ttaataattg aaatgttaat atcaattggt attgcaaagt ttataatttg ctcattttct
     3841 ggaattctat taaatttatc aataacattt tgttcccaat ttttcaaaac ctttgcaaca
     3901 tccttagtta tcttcttaga taataaggct ttatctattg aaattgaatt tgggtctata
     3961 ctacttatta aaaacatcat tgaacccact aaatgctcat ctatttttct atcaaaccat
     4021 tcatttaaca gtttactata attaaaaata cctgcttgga atagtcgaac tgcaacggtt
     4081 ggaattattg gaataattag atgcattgct tgatttctta attcttcgat tgctaaaaga
     4141 ttttgtttaa ttgggccatt ttcatttgtg aaaatgatat ctagggcttt agatagagaa
     4201 attgatttat aatctgcgta taaaacattc gcaatatttt cagtgtcttt caaaactttt
     4261 gattttaata aaagttccca cgagttaata aataaaaata agaaagattc aagtcgatat
     4321 attagagttg gattgtttat tgtttgaagt gcttgaagca ttgcttcatg acttttatgc
     4381 aataatcgcc aaattaattg atctgtttca ggtagatttt ttggaggaac gcctgttttt
     4441 tctgctattt tatatagaat ttctttaaaa gcatcggctg ttactgagct acctgcagag
     4501 aaccattttt catttatttc tttgatttca agttgttcac agatttcacg aactaaatcg
     4561 tgtttctttc cttttgcttt atacttcatt ctgtatgtct tttagatttg cgtcgaacgt
     4621 taccatactt ggtgttcgtg cgggatttca gtgcacaaaa atgtcaacca agaattaaaa
     4681 cccaattgga aaactgaact tgaatttacc accgtaatcc cgtatgacgc caaacgtgtg
     4741 ttatacgttc gttttttcat atttttctgt ttttatgtaa gtataactga tagatacaat
     4801 catctttact ttttgtgtaa gtcctgccgt taaaactatg taggtaaaaa tattttcctg
     4861 ttgatttgcc ttgcattatg taaccatttt gtccatttga aaatgtaatt aatattctta
     4921 tgtatgaacc caaaattatg tcgtatttgt cctcgggctt gccttcagca tagtccattt
     4981 ttgcaaacgt atcaaaaact ttgtttgtcc caccatttac cgaatacttt ctattcaaat
     5041 attcgttgag cttttcaatt gttttaaagg aagtacctat tttggtttcg ttgagaaaaa
     5101 aagcataagt gtcagtgtcg tgtaagttta gaaccaaaac ggattcatca aaaaaggagg
     5161 ttctaaatag caatgactct tctgaatttt caattaggat tgtctgatta cctaaataat
     5221 cccatttgct ttttttgacc tggccgtttt gagaaatcaa taaatcttta ttgtctctaa
     5281 atatgtaagt agttttagtt tctgtcgaac cgtcaaatgc aacccaatga tgattttgta
     5341 tcagcgtcaa ctcgttaagt tgtcggctga aacgttgcag tttcgggatt atgtctgaca
     5401 tgtaatactt cattgtttca cactatgtct ttaaaatgcc ctataactcg tttatatatc
     5461 cggaaaaacc gtataaatac atcaaaaaca gatggataca tatgattttt acccatttgt
     5521 atatcttaac tcaaagttat agaaaaagtt aataatcgtc aaaggttttt tttgattctt
     5581 taaaattttt tttctgatac atgggtgtat atggcttaat cccctatttt ccgggcattt
     5641 tttgcctatg tttttatacc ccgtataagc cgtagtcacc tgactatgct tgcttaaaat
     5701 gcggtttcca gaacacaaac gactaccatc aaataacaaa ctttgactta cagcaagata
     5761 acaataagtt gtcctttaac aactacgtag ttaacctatg ctcaaatcca aacataaaaa
     5821 acaatgctgg gcgagagatt acgaaaaacc taatggaaga atctgacaga aaagcacata
     5881 aaacctgcaa tacgtcaagg aacacnnnnn nnnnnnnngc ctatggcggg actgggcgct
     5941 tcgcgccgct cggagtctta tttattagca gctggcattt tttttaatcg catcatcgtt
     6001 taacatttca gttatccaac ttgtgaattc aggataataa atagcatcaa atttcaacaa
     6061 ttcccaagca tcaataaatg cttttttcga aaatgcaaat tcaatccctt cttcccaatt
     6121 tctccaaact ttttcagaaa tatatccttc tctgtataag tcatattctt cactgcataa
     6181 gtcaaaatac gctctcatat atcttaaagt tttattctta atttcatcag gtatattttg
     6241 ataattaaaa tcttttcgat taatgttctc tggaaaattt aagattattt cttgatatct
     6301 ttttgtgtaa tcagcaaaga agtttagttt taattgctca ttatatattt taagttgttt
     6361 tctattatta actatcgtaa taattaatcc tataatcgcc aagaatatac caattgattg
     6421 aatccaatct gctatttcca tatattaatt tttattagaa caatgaaatt gtgttgtctt
     6481 aatgcttgcc gctaactcac ttataaccat actaaaagta attttataca tacaattttg
     6541 ggggtataac agtacctttg gtaaggttta cttattccaa agatatggtt ctactgttaa
     6601 tttaatgggt
//
LOCUS       DHGB01000062           15226 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_27781, whole genome shotgun sequence.
ACCESSION   DHGB01000062 DHGB01000000
VERSION     DHGB01000062.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 15226)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15226)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15226
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_27781"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         4882 a         3105 c         2210 g         5027 t
ORIGIN      
        1 atcaccttat ttatggtgtt atctttgatt tttatgttat caaatatatt ttcagaaaca
       61 cccgaataat cacctcccca agtcattaaa cacaaatcac catttagata aatattttca
      121 tcatcatcat tttcatcaat aaggatagat tcatcaaaat agacttttat atttttttca
      181 ttcaaaacaa ccttaatttt atggtcaata ttttgagtaa tatttacact acggggaact
      241 aacaagctgg gatatgttcc taataatcta aatccaccag aaaaaatttt atacaattta
      301 acattattat tatcctgtgg ccaaaatggg tcattataac gataagttac aagataataa
      361 ttttttttat ccactgaaga tcgaaaaata tattgctgat ctactccaga aacatttttt
      421 acatcagctt caaaagtata attatttaat actgcattag tatttgcaca aagatatgcg
      481 aacccttttc ttcccacgta actgtgcaac ttaccttgat tagacaccca agtggtcgta
      541 ataccaatat ttccaacaac ttttgtccac tgatttaaat cattatttga aaaatcatca
      601 aaaaataaat cagtagcaaa aatctttgtg gtaaacacaa gtgataaaat tataaaacct
      661 ataaaaatat ttaaacaaaa aagttttgta tttttcatta cataaatata aaacaatgat
      721 agctaatgtc aataagtaaa aaatattaca aaataaccgt actgataata aactataatt
      781 ctaataaaca aacgataaaa ttgttaaaat cacttaaatt aatatctaac tatataaatg
      841 aaatattaat aattgataat tgctcaaacg aagatctcaa aataagtcaa tttaataaaa
      901 ttagtttaat taaaaataaa caaaataaag gattttcaaa agctgtaaat caaggaatta
      961 agattgctaa gaatgatatt attttacttt taaacccgga tacatacctt gaaaacgact
     1021 caataattag ttcgctaaaa ctattaattt caaataaaaa aattgctgca ataggcggga
     1081 aaataaaaaa tataaataat aattacgtcc ccagtgcaaa ctcaaaagca gattttttta
     1141 ctgctatttt tgaatttact aacctgaaaa aaatatttcc aaacaatagt ttttctaata
     1201 aattttggat taaaacaaca caaaaaaaac caatcgaagt tgaatcagtg tgcggagctt
     1261 atattatttt tagaaaaaaa attaacaata aattaaattt atttaatgaa aaatatttca
     1321 tgtatctaga agatattgat tttggaaaca aaattaattc tcttggatat aaggtgattt
     1381 atgatccaaa ttcagaaatt acacatatcg gaggatcaag taataacagt aaatacaaaa
     1441 ctatattaaa atattggtat aaatcaagaa aaatatattt taaagataat ttatcacctt
     1501 tagaattttt ctttatcaat acattatttt caatcgaaga attactgtta aaaatttatc
     1561 acttttttac ccacactcca aatgcctaag atatccctcc ttcttctcac caaaaacgaa
     1621 caggataatt taaagaagtg ggggaattgg attcatgagc tgagctgtat caatgaaata
     1681 gttgtcatcg acgatgaatc aaccgacgac acaattaaaa ttttaaaatc tctttctact
     1741 gataaattat cagtcaatat ttttaaaaga aaactgaatg ataatttttc ctctcagcga
     1801 aattttggcc taagtaaatg tcacaacgac tgggtccttt ttctcgacgc cgatgaaatc
     1861 ccaactccca aaaccataac ctatttaaat aaagtatcac ctaaaaatgg atacaattac
     1921 tcatttaaaa gagacataat ttatttaaat caccctattt cttacggcca atgccttcgc
     1981 gataaaccca taaaattatt taacaaaaac gagggtaaat ttgttaatcc ggttcacgaa
     2041 atttggcaaa gtagcgccac cactatttat agtcagcaaa taattcacca ctattctatt
     2101 aaaaacctca actctttttt agaaaaaatt aatttatatt ccgccattcg agccaaagaa
     2161 ctttttgatc aaaaagacac cgcaaatctc tggcaaatta ttttttaccc caaatttaaa
     2221 tttattgatc tctattttct taaactaggt tttttagatg gagtggccgg tctcatttta
     2281 tctctcagcc tatcatttaa tagctttctt gtccgctcta aactatggca tttgtcacaa
     2341 aaatagccct ttatttatgt ctcgtttttt tcttttttgg ccagcttttt cgtctaaata
     2401 ttttaaacat ttcctttcct ctttttagcc tagcgatatt actcctagcc ttttttaatc
     2461 tcctctctca aaaaaatatt ttaaaaaaac taaatcatcc cgtctcatat tttattattt
     2521 tttctttatt aactctaatt attaattggt ttaagttttc ttttaattta aactctttgt
     2581 tttattggct tcgactagtt tctcttctgt ccttttttat ttttcctcta aaaactgatc
     2641 aaaaaataaa tagatttttt caattagttc tctgggcaag cttaattttt ggttttattc
     2701 aatatttatt ttggccagat tttaccgttt tttccgcctt aaattgggat cctcatctct
     2761 atcgtttagt tggctccttt tttgatccca cctttatcgg tcttattttt ttactattta
     2821 ttcttaaaat atatttccac tattcatcta atcatctatt aatctttttg ccctatctcg
     2881 gtcttgccct gacctacagt cgctcttcac ttctcgcctt atttatcgta ttttctttta
     2941 tttcattaaa aactaaaaaa ctactcctct tcttatctac ttctctactc atcattctga
     3001 ctatctttct cctcccccgc atgcccggag agggaaccaa gctagagcga accagctcga
     3061 ttaaagctaa aatagaaaat tataaagaag gcataaaact cttttccacc tctcccctca
     3121 tcggtctcgg atacaacaat ttaccgataa ttagacgagt aaacagcagc tctcatgcct
     3181 cttccggcta tgacggctca cttttaacca ttgccatcac taccggaatc attggccttt
     3241 tccttttttc caaaatcttt atttcatctc tttatgcctc ttccctttat tggcagtcgc
     3301 ttctcctggc cgtctttatc cattcacttt ttgctaattc tctactctac ccttggattc
     3361 ttttttatct ggcttttgag tttaaatatc gtaagtaatc tcaattgttt ttttcgtttc
     3421 gtttccagcc gggcttctca cgactatttc catattattt ttgccagcat tgagttgaac
     3481 tttcaattta aacccccctt catcgtcgac cactgccagt ttcccattaa tcatcacact
     3541 aactcctttg tcactttttc ccacaatatc aaaatcagca ttttctaccg ttaatttttc
     3601 ctcaactgga ttactcatca ccaggcttgg tggctgatca tcgtaaatta ttgtcactgc
     3661 ttttgacaac tcgctctgac cctctttttc ttttgaggca atggcagtaa aggtatttcc
     3721 acccttatct agggtaatat cggtaaaaga aaagccacct ttttcgtcta ctagttcttt
     3781 accaagagac aaatcatctt tgagtagctc aacctccaca tttggctcgg ccactccata
     3841 aatcgaaaaa tgggcactat tggtcgcctc aaaattaacc accagtcgag gaggcagagg
     3901 aggaaataat ttttcctcac tgtcccgaga gttactcttt gaattaccga gtagcactga
     3961 caatttaatc agtaagggca aaccaaaaac taaaaccgct acaaaaatta aaaccgtcac
     4021 cccacccaaa acgactgttt tcttagtaat ctcttcatct tgttttcgat ctaatcttga
     4081 cctatttttt attttaaaaa attccgacat atattatata ttttaccttt tctcagagcc
     4141 ccttgccaga attgaactga catctgtggt ttacgatacc agagctctac cgttgagcta
     4201 aaggggcact gggagcggct agagggaatc ggaccctcga ctaatccttg ggaaggattc
     4261 attttaccac taaaccatag ccgcactaag ctaaatttta ccacggagtc ggagagtcat
     4321 gccaatttcc agcttatctg ggctatttaa ttttgcttta ttgtcctgcc aaatttttgt
     4381 ccactgataa ccatcaccat attctctaac cgcaattttc caaagagaat ctcctctgac
     4441 taccttataa tctcggtctt ctttttctat atttttagtt acttcttttt ctatttgggg
     4501 taatttcaaa atttgtcctt tctcaattag atccggattc tttaagttgt ttaacttcgc
     4561 aatttcaacc catttaaacc cgtctccgta ctctctttcg gctattttcc aaagagaatc
     4621 atttactact actttaatct catctgcggc taaatttcct tctttaggct gagacagctc
     4681 ttcaattttt aaatcatttt tcaccccggg aatatcaacg ctaccttttc ttttttcaat
     4741 aaaattaacc ataaaaccgc ccactgctac aacgactgcg aggcccagaa acatgcttac
     4801 cacatcatct tttgatttaa agaaagattt ttttgctttt ttagtcatat aagcaaacat
     4861 acctaatttt taacctatag tcaaccagaa atggctattt atctccgtat aataatttca
     4921 ttttccaaac tcgtgatatt ggtaggcatt aacctattta attcttttac aaaattattt
     4981 actgtttcca aattaagctt atatattctt tccttaccgt taactctata atctaccagt
     5041 tttgattttc tcaaaatcga caaatgattt gaaaccgtag cttggctcaa atcaagtttt
     5101 tttacgattt gactgacgtt taattcacca gttctaagcc aaaatattat ttttcttcga
     5161 tttacctctg ctaaagcttt ataaattctg tccacactta gattatatcg acaaacatca
     5221 atatgtcaat ataattaaat agttgaaatt ctagtctcaa agcctcaaaa tatctcacta
     5281 tgaataatta tgataataga caggaaaaaa ccttggtaat tgttaaaccc gacggagttc
     5341 agcgcagtct catcggtgaa gttatcaagc gttacgaaca atgtggtctc aaactagtgg
     5401 ctttaaaaat ggttattcca agtcgagaat tggctctaaa acactattct gtcgatcccg
     5461 aatggccatt aaaaacaggt actaaaagta tcgaagctta caaagctaaa ggcttaattc
     5521 ctcctactga agaccccatc gaatttgcag aaaaagtccg aaaaaatcta ttagatttta
     5581 ttaccagtgg tcccattgtt gctatggtct ggcaggggat gaatgctatt ggtattgtta
     5641 gaaaaattac aggctctaca gaacctttag cttcaaaccc tggtaccatc cgtggtgatt
     5701 acactataga ttcttacagt gcggctgatg ctgattctcg ggctgttaga aatattattc
     5761 acgcttccag ctctgttgaa gaagcaaata aagaaatcaa actctggttc agtgaaaaag
     5821 aaattttaaa ctatcgtcta gtagctgaag aaattcttta tgacgcaaat ttagacggaa
     5881 ttttagagta aagttaagaa taaaaatcct taaaaataga aattctactt gcggacccga
     5941 tgggatttga acccacgatc ttccgcgtga caggcggatg tgctagaccg ctgcaccacg
     6001 ggtcctctag cctcagtact atagcaaaac ttagctcttt tatctattat ttatttaaat
     6061 tactttataa atagctttag tcatcaaatt catttttaaa atagcaccaa aagttataat
     6121 taaatatgtc aagagaattt aataattatc aggtctcgga atatctctcc aaaattgcca
     6181 ttgcttacga aataaaaggc aaaaatcgtt ttcggattat ggcctatcaa aatgctgccg
     6241 aaaccatttt aaactatccc gaaaagcttt atgatctctg gcaaaaagat ccgagaaatc
     6301 tcgacctcat tccccacatc ggtccctcaa ttcttaaaaa aattgactat ttatttaaaa
     6361 ctggcaaaag ttacccctca ctcaaaaaga tttttgtcga cattcacccg gcagttttta
     6421 cctttgaaaa aattaacggt atcggccctc taatcgctca taaattaacc caaactctta
     6481 aattttcccg aaatcctctc cgggctcttg atcagctcgt aacttattgt caaaaaggga
     6541 aaattagaaa tataccttct tttggggaaa aatcagaaga atcaatctta aataacactc
     6601 tctcttttct cggtcgccaa aaaagaatgc cctacaaaaa agccaaagag ttggctggta
     6661 aaatcatttc ttatcttcag tctcgttttc ctgaaactaa ttttgtcacc ctcggctcac
     6721 ttcgtcgcca aagcgacagt gttggcgata ttgatattgc cgcggcttca aacgattcgc
     6781 aaaatatcat tgactatttt ctcacttatc ccgatatggt cgaagttatc gctcggggaa
     6841 aaaataaagc cagtcttcgc ttgctaaacg atatccatat agaccttatg gttaaacccg
     6901 tttcttcctt tggtgctctc ctccagcatt ttactggttc acgtcagcac aacatccttc
     6961 tccgccgtca tgccttaaaa cttggttatt ccctctctga gtatggcatc aaagagataa
     7021 aaagcggtat cttgcatgaa tttagtgatg aaaagaagtt ttatcatttt ttaaatctaa
     7081 catatatccc cccacgggaa agggttgggg gaggggaact tgaaaaatac aaagtgttat
     7141 aatttaatca tatgataatc gccatcgtca tcctcatcct tcttgttctt tatttagtcg
     7201 gctttttcaa cggtctccgt accaccgagg ttagaatcaa cgccgccatt caagaaattg
     7261 gcaatcagct caagcgccaa agtcaattaa ttcccaatct aatcgaatca gtcaaaggct
     7321 atatgaaaca cgaaaaaaat atttttgaag atctcaccgc tgcccgtaaa atgattgaca
     7381 aggccatcaa cagccacgat cctggcgcca ttgacaaggc tcagaccatg ttgaccaaaa
     7441 ctatgggctc acttcaagtt atcgccgagt ctaatcctca aataatggct tcaagtctta
     7501 ttaacaatat gatgaacgag cttcgcgata ctgctgacaa aattatgtac gcccggcgga
     7561 cctttatcga cctttcagct gatttcaacg tcaaaatttc aaccattccc ggcatttggc
     7621 tggcacctct gatgggtttt aagaaaaaaa ctggcttaga aacacctgaa tctaaagata
     7681 ttaccgcagt ttctgagtcc gaaaccaaaa acccagannt taaattttag actttagaat
     7741 ttttttttat ttttatgtct tctttctatc aggaaattca aaataatcgt caacgctcag
     7801 gtctaatcgt taccttattc attatcttta ttgtcggtac aggttatcta cttagctatt
     7861 tatttgacag tcaatatttt ttacccattg caatcgtata ttctttggcc acctctttgg
     7921 gtggttattt tttaggagat aaaatagtct tatccttaaa taaggctata ccagctacca
     7981 gggaaaaata ttttaatttt tataccgtaa ccgaaaatct atcttctgtg gcaaagattc
     8041 ctgttcccaa gatctatgtt atcgaatctg atgctatgaa tgccttcgcc actggacgag
     8101 acccaaaaca cgctgtcatt tgcgccacca ccggcctttt aaacaaactt aaccgaactg
     8161 aactcgaggg ggtaattggt catgaacttt cacatataaa aaattacgac attttgctca
     8221 tgactctcgt ttccgtctta atcggaactt tatcaatttt aaccaatctt tcgagccgaa
     8281 aaatgctttg gagaggcaat gatagagata ataataatcg tgacaattcg ggaattctaa
     8341 tgataattgg ttttgtcttg attatttttg ctccaattat tgctcagtta attcaattag
     8401 ccatttcacg gcgaagagaa tattttgccg atgcctcaag cgtttcttta acccgtcagc
     8461 catctggact aatcagcgct ttagaaaaaa taaactccga tactaccccc tttaaacaag
     8521 cttcgactgc caccgcctca ctttacatca gtaacccttt taagggcaac aaactcgctt
     8581 ccatgttttc cacccaccca cccatcaaag accgcatagc cgccctcgag gccatgatgt
     8641 aattaccgca ttttgttaag atattttaga tttgatttac ttcatctcaa ttgataattg
     8701 ataattgata attgataatt agactatgtc tttcgttaac ctccatcttc acaccgagta
     8761 ttcgcttttg gatggacttt caaaaatcaa aaagctcgtt gctttaatta aagaacaagg
     8821 gggaaccgcc tgcgcgatta ctgatcatgg taatttatac ggtgcgattg aattttataa
     8881 agcctgtctt aaggcagaaa ttaaaccaat cattggttcc gaggtttatt tttgccttga
     8941 atccagatgt gaaaaaactt cccaaaatcg caaaaactat catctaattt tactcgccaa
     9001 aaataatcag ggttacaaaa atttaatgaa actaatcagc gttggttacc tcgagggttt
     9061 ttattataaa ccacgaattg attgggagac cttggaaaaa tatcaccaag gtttaatctg
     9121 tctctcagct tgtacggaag gacaggtagc tcaaaatatt ttaaatgaca attacgaaaa
     9181 ggctaaagat actgctaaaa aatttcaagg cctttttggc gaagattatt atttagaaat
     9241 tcagcgtcac cccggtctta aggatcaaga caaagccaac gaaggtctca tcaaaattag
     9301 ccgagaatta ggcattcctc ttgtagctac caatgattgt cattacctca aaccagatga
     9361 tgcctttgct caggacgtac tcgtcatgat caacacccaa accaatatca acactccaaa
     9421 tcgccttagt atggcctcaa cccctgattt ttacgtcaaa agtccgcagg aaatgacaga
     9481 gcaattcttg gattatcccg aaagtattaa aaatactcaa aaaatagccg ataaatgcaa
     9541 tgttgatatt gaaattggta aatggtattt tcccaaattt aaactccctg agggtaaaaa
     9601 cgccgaagaa actttgactg aaatggtcta ttctagcgcc aaagagcact ataaagagat
     9661 tacctcagaa ataaaagaac gtctcgacta tgaactaaat gttatctgct ccaagggcta
     9721 cgccgcttat tttttaatca tgcgcgattt tattgattgg tgcgaaaaaa atgacacccc
     9781 cactaacacc aggggtagtg ccggaggctc tctagtctct ttcgtccttg ggattgtcac
     9841 tgttgacccc ctgatttata accttccctt tgaacgtttt ctaaatatag atcggccctc
     9901 acctcccgat attgatcttg atattgccga tgaccagcgc caacaaatgc ttttccacat
     9961 tattgaacaa tatggaactg attcttttgc ccaaatctgt acctttggcc gaatgatggc
    10021 cagaggggct gtccgcgaca ccgcccgagt ccttggatac gaatacgctg ttggcgataa
    10081 aatcagtaaa ttaattccta tgggctcaca aggttttccc atgagtatcg ataaagcgct
    10141 agatacaacc ttggatttaa aacaacttta caacaccgat tctgattcca aaaaaattct
    10201 cgacacagcc aaacaaattg aaggctgtgc ccgacatatc tctgttcatg cctgtgctat
    10261 cgtcatttca ccaaccaaaa ttaatgattt ttctccaact caaaccgaaa ctggaggcga
    10321 aaaaccgatt actcagtacg aaatgcacgc ctgcgaagat gtcggcttaa ttaaattcga
    10381 tattttagga atcagaaatc tttctttttt acgtcaagcc attattaatg ttcgcaaaac
    10441 cagggatctt gaaatcaatc tccgaaaaat tcctcttgat gatcaaaaaa cttttactat
    10501 gctctctgcc ggtagaacta tgggcacatt tcagctcagc ggggagggaa tgaccaagtg
    10561 gcttaaagaa ctaaaaccca atcgggttga agatttaatg gccatggtcg ctctttatcg
    10621 ccccggcccc atggctatca ttccgcagta tattgaaaga aaaaaacatc cagaaaaaat
    10681 ttcctatttt gaccctaaaa tggagaaatt tttaaaaagc tcttatggcc tgctcgtcta
    10741 ccaggatgac tgtctttata ccgccatcga actggccggt tattcctgga cagaggtcga
    10801 taaatttaga aaagctatcg gtaaaaagat tcctgaagaa atggcggctc aaaaaattaa
    10861 atttattgat ggttgcgtta aaaatggcta ttcaaaaaac aaagctgagg aagtatttaa
    10921 attaattgaa ccctttactt cctatggttt taacaaagct cacgccgcct cttatggtat
    10981 gttggcctat cgaaccgcct atatgaaggc caattaccca gttgaataca tgtgtgccct
    11041 cttgactgcc gaggccaacg atatcgaaaa ggttagtgct ggcatcgaag aatgccgctc
    11101 catggggatt attattctcc ctcccgatat caattattct caaaaaggtt ttgaatttga
    11161 aaaaaatccc gattctttag atcatctggc cattcgggtt ggcctctctg ccatcaaaaa
    11221 cgtcggcgat attgctatcg atttgattgt cgaagaaaga aatcaaaatg gaatttataa
    11281 aaattttcat gatttttgtc ttcgaagtaa tgcccaaaaa gtcaacaaaa aagtccttga
    11341 aagcctaata aaagtcggtg cccttgatag ctttggtgaa agaaatgcta ttttagcctc
    11401 ggtcgatgaa attagaaatc agtgcagtaa acttaacaat aaaaaagaca ctggtcagtt
    11461 tggtcttttt gacaactcag acacccccac tgttattcct caagatattt tcatgaaagt
    11521 agagcccatg cctcaaagtg aaaaattatc tttagaaaaa aatttacttg gagtttatgt
    11581 cactgaaaat cctatcagta aaattttggc ctcctttaat tcagttaatt tattaaaaat
    11641 aagtgatctt caagggaaaa ataataatag ccaggttaaa tttgtcgcca taattcataa
    11701 atgcaaaaat attagaacta aaaagaataa tgcccaaatg gcttttctca ccctcgggga
    11761 cgatagtggc cagatagaag ccgttatttt tcctcaggct tatgaaacat acaaaaatat
    11821 tattgaagaa aataaagcct tttatttcga aggcaaaatt tcactccgag atgacactaa
    11881 atcagttctt atcgatttac tctccacctc tgcacctaaa actaagtcta cttacgattt
    11941 tgtcattaaa gtaccacccg gtacctccca atctcaactc atggatcttg atcgtcttct
    12001 taaaaaaaat caaaatggcc atcgtggttt aattattctc ccaaacggca aagaattacc
    12061 tctatcctat ggagtcaatt acaatgacaa tcttaagacg caaattgatg aaatattttt
    12121 cccatccttt tcttagatat tgattgccta cgtaaattaa tataaaatac caaagtttat
    12181 agattatttt tttatggcca ttaagtttac tcatcaagac acagaattcc atgtcgggga
    12241 tactgtcaaa gttaattata aaattaagga aaaagacaaa gagagactcc aagcctttga
    12301 cggcatcatt atgtcaatta gtggtacgac tgaaaataaa aactttttag ttgctaaaaa
    12361 cgccagcgat ggtgttaggg ttgaacgtat ctttccaatt aattctcctt ggattgcctc
    12421 aatcaaaaaa atgcgtagtc caaaacgtct tctccgtcga gccaaacttt attttttgcg
    12481 taatccccac accagaggta tttaaacaaa acttttcttt tcttcatttt cttttcttat
    12541 attgaaaatt gaaaattgat aattaaaaat ttattaaggg tccttagctc agtggtagag
    12601 cagttgcctt ttaagcaatt ggtcgcgagt ccgatcctcg cagggcccac ttaatgcatc
    12661 tctacggctt aattatcggc gttagcatta tcatcgggat taaccacttt agtcgaaatc
    12721 aaaaagttat ttccaaaaca aaagaaaatt tttttattta ttctctttta tttttttcga
    12781 ttattggtgc cagactttat catgttatcg actactggaa ttattattcc caaaatacca
    12841 atcaaattta tgccacctgg aatgggggac ttggtattta cggtgccctc attggtggaa
    12901 tactttatat ttttttcttt tctctctttt cccgaatctc gtttctaaaa atcttagatt
    12961 caattactcc catcctccct ctatgtcagg ctatcggcag aatcggtaat ttttttaatc
    13021 atgaaatccc cgtctggtgg ctcgaagcat ctttaaattt aattttattt tttattatta
    13081 agtctgaagc tctaaagaac tatagttcta ccgcattata tttaattggt tacggctcga
    13141 tcagattttt tattgaattt tttcgaaatg atacctggca agttagctct tttaaaatag
    13201 ctcaagttat ttctctatta tttatcttta ttggcttaat tattttaaaa tctactagaa
    13261 agtccaataa tttgctaaaa taagcagcca cggctccatc gtctagtggc ctaggactcc
    13321 tggctttcat ccaggcaatc gtgggttcga ctcccactgg agtcacaatt cgtttttaat
    13381 cctccacttc catcatcaga ccaaaatgat cagaaagatg gatgtttttt cttttcagcc
    13441 ggtaaagatc ctttagaatc actttatggc taatattctt caaatctttt gaaagtaaaa
    13501 tatagtcaat ccgagaatct tttctaattt tataaaagtt ttttctttgg gtattttctt
    13561 tggaaactgt aacgatctct ttccctcgta aagggtcata taaatttgtc ttttcctcaa
    13621 actgttggta catactatcg gtaaaagaaa tattaaaatc tcccgccaca attacttttt
    13681 ctttttctaa attcaaatta tttaatagct ctgaaaactg ctttaataaa actctttttt
    13741 ctttagaaga aacattttta taaagactta ctaggtgagt atttattaca gtcactattt
    13801 ttccctcaat ctccaatcta attttttgat agcctttttt tagaaaccga tcagttaatt
    13861 gaaaagaaat ggaggtacct tggctattaa atttgatata ttcactactc acaatcggat
    13921 acttagaaac aaagaaaaga ccacctctat ttaatttttt tctcggatta aaaaaagaat
    13981 aatagcctaa tctttcaaaa ctttttgaaa tccttttaac cctttttaaa aaaattagtt
    14041 cttgaaagca aattacatca ccgtctaatt taattatttc cgatacaaga tgattaattc
    14101 tatctgttct cttgggtgac aagggagaat cgaaacaatt tatggtaatt attttaattt
    14161 tcatttattt tcagattttt attatatacc aatttgcttg atatactttc attgtgattt
    14221 atatttttca tgggaaccac acctctcaaa gctataatga attttccgaa cttttgaata
    14281 attataaact acacgaaaag ttccaccaaa atagcaaaaa tctagaaatt gattctctaa
    14341 atcgttttat taacacccct tctctttttt cagaaacaaa agtggttata atcgaaaatc
    14401 tcttttctct cttaaaaccc caacttgagg ctgtctccaa attaatcaat tctcgtccaa
    14461 atttagatta tttattttgg caggataaaa aaattgaaac tgttaaactt aaattatttc
    14521 ccaaatcaac ggtcaaaatt tttgtcttac cggaagtatt atttagtact ctcgattcaa
    14581 ttcgacctca aaataaaaac gaatttgtta aaaaatatca aaatttagtc tctattttac
    14641 cctttgaatt aatcctgttt tggtttaaaa atcatcttcg acgtcaacta accacttatt
    14701 caaaattttc caccacctca ttaaaagaaa cctatttaaa tttaattaaa cttgattatc
    14761 gttcaaaaac tggcaaacta ctagaattca aagaaaactg tttggaaaga ataatccttt
    14821 ctcttatcga ctccgggccc taatttctgc tattattttt tatatgactg cccaaatcat
    14881 tgacaaaccc tggggtcagg aaataatctt gagcactccc gatcttcctt acactgccaa
    14941 aattttagaa ataaaggctg gcaaacgctt atctctccaa taccacgatc aaaaactcga
    15001 gactctttgc cttttctcgg gacaggccaa tattatttgg ggccaagatc aatcaactct
    15061 aacaaccgaa aaaatgatta aaaatcaagg ctacaccatt acccctggta ccattcaccg
    15121 ccttgaagct attagcgact gtcgattttt cgaagcttca accccagaaa ctggcactac
    15181 ctaccgcctt gaggacgata ctgagcgtga aaatgaaacc gaaacc
//
LOCUS       DHGB01000063            6504 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_32510, whole genome shotgun sequence.
ACCESSION   DHGB01000063 DHGB01000000
VERSION     DHGB01000063.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_32510"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         2050 a         1313 c          949 g         2190 t
ORIGIN      
        1 cctatgatgc tacttctatt gaggaagatt cctggaaaaa acctgaaatt agacaaaact
       61 taaatggact caaaattggt ttacccctct cttattttcc ctctcaaatc caacccgaag
      121 taaaaaaatc aattttagaa gcagctgaaa ctttaaaaaa acttggggcg caaattttgg
      181 aattaccaga tgttttagat cccaaatact cgattgccgt ttatactatt ctccagcgct
      241 ctgaggtttc cagtaattta gctcgttttg acgggattcg ttatggtcac gaccgttctt
      301 attttgggac cgaagccaaa cgtcggataa tgcttggcac ctattctttg tctgctggtt
      361 attacgacgc ctattattta aaagctcaaa aagttcggac tctaatttgt caggatttcg
      421 ataaacaatt tcaaaaagtt aatgcgatta tcggtccagt ttctcctact accgctctaa
      481 aacttggtgc tgccaaaggt caggccatgt ttggtgaact tcaagacatg ctggtggaac
      541 cttcaagtat tgccggttta cctggtattt caattccttg cggttttgac aaaactgctt
      601 tacccatcgg ttttcaaatt atagctcctc aaaaacgcga agatcttgtc tatcaaattg
      661 ccaaaatttt cgaagacaat actgattatc atacaaagag accaaaatta tgaaaatcac
      721 tcccattatc ggacttgaag tccatgttga attaaaaacc aaatccaaaa tgttttgtag
      781 ctgtcgtgcc gatcattttc atgtcaagcc caacacccac acttgtcctg tctgtcttgg
      841 tctccccgga gctctacctg ttcccaacaa aagggcctgt gagtggtgtc ttaaattagg
      901 tctagcttta aattgctcta tcaatgagca gtcttttttt gaacgtaaaa attattttta
      961 tccggacctg gccaaaggct atcaaattac tcaacttcaa aaaccttttg cgattaacgg
     1021 ctatttaaat attgagggtc acaaaattag aatcaaccgg gcccatatgg aagaagatac
     1081 gggtaaatcc attcatattg acggctcgac tctgctagat tttaatcgct ctggtgttcc
     1141 tctggtcgaa attgtttccg agccagatat tcagtcggcg gagcaggcca aaaagtattt
     1201 gaataaacta caacaaatta tccgctacat cgggatttct gacgcagata ttgaaaaagg
     1261 ctctatgcgt tgtgaaccga cggttaatgt ttgtctcgat gacaaatttt atacaccttt
     1321 ggtggaaatt aaaaatgtcg cttcacttac cggcgttatg aacgccataa actacgaaat
     1381 taaacgtcaa atagatgctt atcaatctga tggtattacc aaaaattctt cgaataaaac
     1441 taccagaggc tgggacgccg ataaaaatca aaccttttta cagcgggaaa aagaggggag
     1501 tgccgactat cgttattttc ccgagccgga tattccacct attattttca ataaagagca
     1561 aatagaagaa attcgtcaaa gctttcccgt tttacccgat gaaaagattg ctcagtacaa
     1621 gttgctcgga ctttctgatt acgatgcctt tattctcgct gatactaagg aatccgccga
     1681 aattttcgac aaaataattt ctatagagtc ttctcgcgaa tttgccaaat tttctgccaa
     1741 tttattaacc ggtccgttaa aaaccattaa aattgatatt aataaaatta atccagacta
     1801 ttttaaaaaa ttatttgacc ataaattaga tttctcttct actgtcatca agcagttaat
     1861 tttggattcc tatcaaaccg gtcttgatcc cttggagatt gccaaagaaa aaaacctttt
     1921 tcaagtttcc gacactaata ccttagagca gtttgctaaa gaggtgattg aggccaatcc
     1981 caaagctgtc gaagattatc accaaaatcc cctggccatc ggctttttag ttggccaatt
     2041 aatgaaagtt tctaaaggta gtgccaaccc tcaaacaggc aaagaaattt tggaaaaatt
     2101 attaaaataa atttggtaaa ttaaatattt taaattttct ggtgagtagt tataaaagcg
     2161 aatatcaaaa actcaattct gaacaaaaag aagctgtcnn gctctaagaa taggtaaaat
     2221 tagagaggaa acccaagtca atcccagtaa tattttatgt ttgaccttta ctgaaaacgc
     2281 cgctctaaat atgcgtcagc gtctcttgga gctgatcggg gctgatgcct acggtgttcg
     2341 tatcgccact tttcacgctt tttgtaactc tatcatcaaa gattaccctc agtattttct
     2401 taaatctgct cttgagtcca aagccattga tgatattacc aaaattgaag taattcgtag
     2461 tttaatcgat aagttgcctc agagttcacc atttttcaat ttaaattatc cttattttta
     2521 tcagaaagaa ataatttcct caatttcttc tttaaaaaaa gaaaacattt ctcccctccg
     2581 ttttgaggag ctgataaaag acgccgccaa ttttgtcctc ttagcctctc ccttgtctga
     2641 aaaacttaaa gagcttcgtg cctcccccaa aagcgccaag accataattt ctctcataaa
     2701 aactctcagc tcccagctcc atccattacc cctttatcag gttcgaatta attactttct
     2761 tcagctttat gaatcagaga cccttactct ctccgaaatt aaacaaaaaa ccagagattt
     2821 cattgataaa agttcagatt ctttaataaa aatcagagat cttttggtaa tttataaata
     2881 ttatcaagaa gaattgacaa ggcgcggtct ctacgattac gaagacatga ttctgtgggt
     2941 cattgtggct cttcaaaccc atcctgaact tttattaact tatcaggaaa attatcagta
     3001 tctacttgtt gatgaatttc aagataccaa ttcctcccaa atggaaattc ttaatcttct
     3061 ggttaaaaat cagaataacc ccaatatttt tgtggtcgga gatgatgatc aatcgattta
     3121 tcgttttcag ggagccaatg tcgaaaatct ttattctttt tatcaaaaat ataaaaagat
     3181 ccttaaagta attgttttaa aaaataacta tcggagtcat caattaattt tagactcttc
     3241 aacggagctt attaatcata atcaaattcg aattagtcaa tatattggca acattgataa
     3301 gtccttaatt gccactaaag attatgatcc ggatccgatc aatctttttg tcggggacag
     3361 caatgaggag gaaaattatt gggtagttaa aaaaataaaa actttacttg agtctgggga
     3421 aaaagccaat gagattgcca ttctttaccg taacaacgcc gatattaatg atcttctctc
     3481 ctttctttct caaaacgaag tattatatct ccgttctgat ggcgtaaata ttttggaaac
     3541 tttggaaata gagcagctct taactctact tagatactta gccagtcctt cagacaatac
     3601 tcttctggcc catcttttat catttaattt tctccgcttg ccttcaagcg atctttatcg
     3661 attattcatt ttaattacta aagaaaaatt aattttatct gaggtaattt ttgacaaaca
     3721 gcttttaaaa gaaaataaat tttctctaaa atttataaag cgaattaatc gttttaccga
     3781 tgctttagtc aagactcaaa aagatgccaa taatcttgtt cccgatgaat tatttaatct
     3841 tattatccga cgctttaatt atctaaatta ccttttaaaa acccaaaata tctctctttt
     3901 aaaacaatta aatactcttt attcccattt aaaacaaagt ttatcggttc aaaaaatctc
     3961 tctctttgat tgggtcaata agttgtcttt actcagtgaa aataatttgg ctttaaattc
     4021 cccacctctt cagtcagatt ttgaaaattc aattcattta atgactgttc ataaagccaa
     4081 gggtttggag tttaatcatg tttttatcat caaagtttta tctgaaaaat gggataaatc
     4141 gtcctcacgt tctcttatta aattacctct tggagttctc ctttatgata ttgaagctga
     4201 tattttagag gaagatcgtc gtctttttta tgttgctctt acccgagcta aaaaacaaat
     4261 ttatctctct tattctcgtt ttaacgactc caggaaggaa caactcgcct cccgttttat
     4321 ctctgaaatt aaccaaaaat ttattgaaaa aaagtcctca gacccagcca tagaaaaaaa
     4381 caccttgctt actttgtatc atccttctct ccccaaaatt aaatcaactg ttttatcttc
     4441 ttatttgaaa aactatttat cgacaagtta ccgattcaac attacccatc tcaattctta
     4501 tcttaaatgt cccctctgtt ttttctttaa aacaatttta catttaccct atcccaaagc
     4561 caaagccatg tcctttggta ctgccgttca tggcgccctc gcttatttaa ctcaggttta
     4621 caaaaaagaa aattttctca taaatcagga aaaatttctc tctattttta atcaaaattt
     4681 aaagcaggaa aatttatcac cgactgattt cgaacaacta gaaattcatg gtcgggaaat
     4741 attaactgct tattatcagc actataaaga gtctttcaat ggcaactgtc taactgagca
     4801 tgattttaaa ttcaaaaatg ttcgtcttgg taatattccc ttaactggaa aaattgataa
     4861 aatcgaattt ttgtccggaa atcaggttaa tgtcgttgat tataaaaccg gtaaacctga
     4921 ttcaaaatct cagcagctta gcccagaggg agattatttt cgccaacttg tcttttataa
     4981 attactctgc tcggaggcca aaggctttcc ctacgaagtt ttttctggca ccattgattt
     5041 tatcgaaaaa aatagtaaag atcagtttgt ccgcaaaaac tatctactaa caagtgataa
     5101 tgttaataaa ctaaaatctc aaattactga agtttttgac aaaattgtta accttgactt
     5161 tacccctaat cccaaatgcg acgactccga ccacctccat attttatttg ataaatattt
     5221 tcactaaacc tgtttttcta ttttctctgt tacaacccta ccgccctgcc attgacctcg
     5281 atacaaattc gtttttccca tgatttcata aaacatcata tgaattatcc ttgcaccaat
     5341 ttcaattttt accgggcaat taccactgtt aaataaagtg aagcttattt ctccactata
     5401 tcctggggct acatttccac ccgaaagaat tactccaccc cgatataaag tgcttcttaa
     5461 ccaaaaattt gctgttaaat tttctggtaa attaattttt tctataatcg ttaccaatac
     5521 ttgttgtccc gattttaatt caaaaattcg gctttctgtt tcgtcatatt ccattaataa
     5581 tttagcttct actgattttc ggtcagtcac ccctaaaaat gtttccccgc ttatttccca
     5641 aacttttccc agtcttaggt cgaggccaca tccctctgga ttggttaatt ctctttctga
     5701 taacccctca actaaatttt tttctttaac taatttcaac aactcatcaa ctcctaaaat
     5761 catatataaa tcatagcaca aacaatttta acccctccag cctacggcca tttccccttg
     5821 acaggcgagg cgagaacaac tttctaatat tttttgtctt accttgttaa ggtcaaataa
     5881 tatttcttga ttggtgaatc taattgtttg tattccaatt tgagataaaa atttatctct
     5941 caatatatcg ttttctttct ccttaatgtg tgatcatcca tcaatttcta taaccgaatt
     6001 taattttaaa cagtaaaaat ctaaaataaa tctatttatc ggcttctgtc tactaaattt
     6061 aaatcctatt tttcatttct taatattttt tgctaaatga tatttttcgc ttccgttgga
     6121 ctatgtctat ttttcttagc catttgaatc aaaccgattg tgtaattaat atttccatga
     6181 attggattta cagcctcaat ttcgattttc ttcttcacaa aataagtatt tattttttag
     6241 cctcgcctgt caaggggagg tgtcctgaca taggtcggga cggaggggtt agattaaggt
     6301 aaaataatct atgacccttg tttatatcct tctcgcttta atcctaattg ccgtccttta
     6361 ttttatttat ctccaaagga tgaataaacc gagtgaaatt gatttatcta aagctatcga
     6421 cactctggtc aaacaatcaa acgataagtt aaacgacaaa aatctccaaa ttcaaaccga
     6481 tctttccaac aaaaaagatg ctat
//
LOCUS       DHGB01000064           15499 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_26352, whole genome shotgun sequence.
ACCESSION   DHGB01000064 DHGB01000000
VERSION     DHGB01000064.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 15499)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15499)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15499
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_26352"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         4700 a         2836 c         3185 g         4778 t
ORIGIN      
        1 gttagtttat cgtaattata catttgaact acattttcaa ttttacgatt taattcaaga
       61 ataaccgaga ctacgattaa aactgaagtt ccaccaatac ttagtgaagt tatccccgtc
      121 actttttgaa tgacagaagg aatgacggca atcatcgcta gaaagaaggc tccaataaaa
      181 acaacccgat aaagtaaata ttcgagtctt ttttcagtgg caactccggg acgaattccg
      241 gggataaaac cgcctgattt acgaagctct tcagcgacat cttttggttt gaaaacaaca
      301 gtggagtaga aaaaagtaaa gacaattacc atcacaaaat aggtaactaa atacaaaata
      361 ctggtaccgc taaaagctaa tgataaatta cgggcaaaag tggcgatggc ttggttgcct
      421 acccgactaa gagcttggcc taaaagagag ggtaaagaaa cgagggaaac ggcaaaaata
      481 atgggcataa ctccagccac gtttatttta ataggaagat aggttgtctg ggctcttttg
      541 gcataagaaa ctggaattct taaaacggct tcttcaatgg tgacaacagc aaaaataatg
      601 gccacagcca gagccaggaa taagattaaa ttaaatagag tttggggatt ggaaaaatca
      661 gccaaactta aagattgaat ggctgatact ggtaaacggg aaataatccc ggcaaaaatc
      721 ataactgaaa taccattgcc aatcccaaat tcattaatta attctcccaa aaagatcata
      781 atcatggtcc cagtgaccat ggaggcaatt aaagccacaa taactaaaat tgactgctgg
      841 ccaataatat tttgagattt taagatgccg tacataccaa gagattgaaa gatagaaaga
      901 gggatggtaa taatccgggt gtactgattt aatttggcct gaccatactc cccttctttt
      961 tggagctctt tgagctcggg gatgacaaaa cctaaaagtt ggagcatgat cgaagcattg
     1021 atgtatggat ttaatcccag ggcagcaatg gaaaaattag cgagagtacc gccagaaaaa
     1081 atatcaagaa gtgagagaag ctgattttga gcaaaaaatg attttagtaa agctagattt
     1141 accccaggaa caggaatatg agcaatgagg cgaaaaataa ttaagatacc gagagtaaat
     1201 aagatttttt ttcggagtag aggagctttt aagccctctt tataaagaga caatgatttt
     1261 gtcagataat tcatgactct atcttaccac ctgaagctaa tacttttgaa gacgctttct
     1321 ccgaaagctc gaggccttta aaagttaatt ttttatcaag atcaccagta gataagattt
     1381 taactttttt ggttaaatct cttttggtag tgcgaataat tttttcaaaa atagtttttt
     1441 tatcaacgac atcaccagct ttaaaccagg aattaatttg agttaaagaa acaatgtagg
     1501 tatcgtttag aggcagtaat cgatgttttc cacgtaggaa aggtaatctt tttagccagc
     1561 cttttttaat tttggtgccg tcaaaagtta acttgccatg acctctgaca ttatccccct
     1621 tggcgccgcg accagtagta tggccaccaa caccggaacc gatacctcgg ccgacacgtt
     1681 ttgactgctt ggtagtagtc ttttttaaag aagataaaat atccatatta ttttttaaga
     1741 acctctaatg ctttaatagt cgcccagaca ttgattgatt tatttcttga accaataatt
     1801 ttactgacca cgtctttgat tccagcaagt tcaacaacgg aacggatggg accaccagcg
     1861 attaaaccgc ttccctcctt acctggtcga agtaggataa ccgcgccttt aaactttaat
     1921 ttaatagaag caggaatagt gccgttaaca ataggtactt cgatgagtga ttttcgaccc
     1981 cgattgatcg ctttttgaat ggcatcagct acacttttag cccggccatg acccactcca
     2041 actcggtttt tcttgtcgcc ggtaacgact agggcggtaa aagaaatttg attaccgcct
     2101 ttagtttttt tagaaacacg tttaatttca acgactttat cggtaaattc actgactatt
     2161 ttttcttctc tgtcttggta catagttttt taaaatttta gacctccgtc tctggcaccc
     2221 tcggccagcg cttttacccg gccgtgataa cgatagagac cacgatcaaa tctaataaaa
     2281 gtaatttttt tagattgagc taatttagca atggcagaac caacggcctt tgactgctct
     2341 gatttagtcc ctttggtttt tacggttttg gtggaggcgg aaactaaggt tttgccgtgt
     2401 ttatcgtcaa ttatttgagc ccaaatatga caattactgc gaaaaaccga aagacgagga
     2461 atagttatat tttgggataa tttaccacgg actcgatgaa ggcgtctggt tttactttga
     2521 ttaagggtgg tcatattttt ttaagctttg gcagttttac cagcttttaa ctttataaat
     2581 tcatttagat aacgaattcc cttgcctttg taaggttctg ggggacggat ttttcgaata
     2641 ttgctggcaa tttgaccaac aacttcgcga ctacagccgg aaacagtaat tttagtttct
     2701 tcgttaacgg taatagtgat accttcggga gcaaaaataa aaacaggatg aatataacca
     2761 gcgagaacgg ttaatttatc gccggagact gaggctttgt atccggtacc acgaatttct
     2821 aattcctttt tccagggagt aacaactcct ttaacgatat tatttaaatg agcccggaga
     2881 gaaccgtggt tcgaacgggt ttgtttgtct tcggcgagac gcgaaacttt taattgatta
     2941 ttttcaacac taacttgaat ttttggtgga atagaatgag tcattgtccc catgggacca
     3001 gtaatcgtaa tgacagaggc ttcacttttg acagtgacgt tttcgggaat agtaataatt
     3061 tgtttgccaa tgcgtgacat agatatttac caaatttcgg caacgagctc tccgccgagt
     3121 tttttggtaa cggcttcttt ttgagagagc aaaccactgg aagtggaaat aataattaac
     3181 gaatttggag tttttcccca aggaattgaa gaagaagtac agtagagtcg gcgagatggc
     3241 cgagaataaa tttctaaatc ctgaattcgg ggctggctaa tcgagataat tctctctttt
     3301 gaatcaacag taaaatcggc aataaatccg tgttttttaa gtttttcaag aatggaaaca
     3361 gaatactttg ataaaggagc ggaaatactt tttcgaccag cccttgaacc gtttttgatt
     3421 ctgattaata agtcacagga gagagaattt atcataggtt taccagctgg ctcttttgac
     3481 cccaggaagg gcgccagtgt ttgctaattt tctaaaacat aaacgacaaa gtccaaaaag
     3541 acgaatatat gatcggggcc ggccacaaag ctggcaacga ttatgatgtc gaacagaata
     3601 ttttaatttt tttagttctc taactttttt tgcagtggta gccatattta tttttcaaag
     3661 ggaaatccca gggtggataa taacattttt gactcttcga ttgaagaagc agaaatacat
     3721 aaagtcactt caaccgatcg aattttatta atgacgtcta gatctatttc ggaaaaatag
     3781 gtttgatcac gaatggtaag tgaataattt ccctgcttat caaaagaatc taagggtaag
     3841 cccttaaaat cgcgaagacg gggcaaaact aagttgaaaa gcttattgat aaaatcgtac
     3901 attctggcgg agcgaagagt cactttgtaa gcgagaggtt ctccctgacg aagcttaaag
     3961 gcagcaactg attttttaga acgggttaag cgaggttttt gtccagtaat agccattaat
     4021 tctttttcag ccgcggctaa aagctctttg ctgtctcgac catcagaaac acgatagtta
     4081 atgacgactt tggttaattt aggtaaagca ttaggatttt ttatccctag tgtctgactt
     4141 aatttttgat ttatttcttt gagataaggt gacatcatat ggtttatttt ttactgggca
     4201 tgataataga tttacactta cgacaaattc ttgatttgtc accgttttta tcaatttgat
     4261 aagcaactcg aacttttttg ccacagctgg gacaaattag cataacttta ctaactttca
     4321 gagctctttc tttttcgata atacctccct tctgaccttg agtcggtttt aaatgttttt
     4381 tgaatagatt aagtcccttg acaattattt cttgtttttt agtaaaaacc cgaagaacct
     4441 caccactacg acccttgtct tttccaatca aaatttcgac tttatcacct ttttgaattt
     4501 tcatattaat aaacctcctt agccatagaa gcaattttgg tataaccagc atcttttatt
     4561 tcgcgggcaa ttgggccaaa gatgcgtgta cctcgaggat ttttttgaga atcaataatg
     4621 acagcagcat tgtcggaaaa acgaatataa gaaccatcgg ggcgacctaa ttcttttttg
     4681 gtgcggacga tgacggcttt aactttttct ttctttttaa caaggccatt gggcatggct
     4741 tctttaacaa cagcaagaac tgtgtcccca attccagctt gtttgtgttt ggaaccaaca
     4801 taaatatgca taatttcgag agatttagct ccgcaattat cagctggttt gagtcgggtt
     4861 cttagttgaa gcatattact cggcggtgcc tgatttaata atttctaagg taatgaatct
     4921 tttgagttta ctaaggggtc ttgtctcgcg aactttaaca gtatcaccag ttttggcttt
     4981 gagattattt tcagcgtata tttttttgtt tttcttgacg atttttttat ataaaggatg
     5041 acgaaaggtg taggacaaag agaccacaat agtgttggcc attttgtcgg agacaacaat
     5101 gccggtaaaa atttttccag ttttaggatt aattttgtct gtcattttga ttaattaatg
     5161 tcgaaattag ggctatttct gactgaattt ttttaaaaac agaagtatct ttttggttgc
     5221 ctagatactg cttactcttg gcctccatca atttttttcg aagatcagcc aaattagtaa
     5281 tcaattctgc ttttgttttt tgcttgtagg caattttatc agtttttttc ataaaatctt
     5341 tagtaaatta ttttctttta acgactttag taataactgg taatttagct gcagccaagt
     5401 ttagagcctc atgagcctcg gcttcagcaa taccggtaac ttcaaataat atacggccgg
     5461 gacgaatcac acagacataa cctttgacat cacctttacc ggcaccaagg ggggcgccag
     5521 ctcctttttg agtaaagggt ttgtcgggga aaatacgaat ccaaatacga ccatttcttt
     5581 tggtagcatg agcaatagcc cggcgggcgg cttcaatttg attgctggtt atccagccgc
     5641 aggtcatggc ttttagacca tattcaccaa aggcaactaa attaccttga gaattgcctc
     5701 tcattcggcc tcggaactgc tttcggtatt tggtgcgtga tggttgtaac ataattagat
     5761 tattagatta agagattaat agtaaatagt aaaatttaag ttaagcataa ataaacttta
     5821 actccgatat atccagatcg ggtgagacaa ggaacctcgg cataatcgat tctggattta
     5881 atggtactta ggggaacttg accttgatgg aatttttctc ggcggctaat ttcggcacca
     5941 ttgatacgac cggagagaat aactttgata cccttgacac cagcggccat tgatttttca
     6001 attgcagaga gaacgacccg gcgataaggc atgcgcttgg cgagttgaaa agaaattttt
     6061 tcagccacta attttgccga taaatcagta tttttaaatt cttcaacttg aatgtcaatg
     6121 ttggttttgg ctttttgttt accaattatt ttctgaactt caagcttggt cgcctcaagc
     6181 tctttgccac cacgaccaat aactaaaccg gggcgggaaa caattaaaat taattttatt
     6241 tttttagctg agcgttcaat tcgaatagca acgataccgg ctgaagatag tttcttttgc
     6301 aagacctggc gaattttata atcttcgaga agaagctctt tatatttagt tttgctggca
     6361 taccaagaag actgccactc aggagaggtg gaggtggttt ttagtctgaa agcgattgga
     6421 ttaacttttt gacccatata tttatttttt gacttgacct ttaacgatta tggttaagta
     6481 gctatgtcgc ttgataatga cccctctggc aaaatgagag ccatgagatt tatccaggcg
     6541 tttagtcttg ggaccttcgt tgacggtaat tgtatcaaat ttaagagttt cggggattaa
     6601 atcataatta tttttagcca ctgaaatggc agttttaaga gccgaagcca aaacccgagc
     6661 cgccttggta ttggttaaag acaatcttgt taaggcaact gaaggtgata tttttttgat
     6721 ggaagtagtc aaaaggcgaa gttttcgagg agaaatttta atatttttat cggtatattt
     6781 cgatagttta aattctggag tcccgccgat gcgggatttc gcttcgctta actcttttac
     6841 tggagttttg gcaactgcaa cttttttggg agtagttttt ttgatatttt ctttagccat
     6901 attttaagtt ttctcaataa cccgtttaac cacccgacca tggcctttaa atgttctggt
     6961 taaagcaaat tcaccgaaac gatgaccgac catgtcttca gtgacataga cttcaataaa
     7021 tttatgaccg ttgtgaacag aataatggtg accgacaaaa tcgggagaga tctgactgcc
     7081 gcgagcatat gttttaatag gattggcatc ttgactagcc gctgtttttt tgactaattt
     7141 agcatcaatg tagggacctt tttttgatga tctagccata gattactttt ttcttctttt
     7201 aataattaat ttgtttgagg cttttttatg ttttctggtt tttttaccca tagctggttt
     7261 accccaggga gttttgggat tcatgccgat actggaacga ccttcaccgc ccccgtgtgg
     7321 atgagagtga ggattttgag ccacacccct aactgctggt cgaatcccac gatggcgaga
     7381 atcaccggct ttgactaatt taatgtttga atgatcgaca ttaccaattt gaccaatggt
     7441 agcacaggca tcaccaggaa aaagacgaag ctcaccagag ggtaatttaa tagtaacttt
     7501 gttgacatcg attgattgaa taaaggccgc agaaccggca gatttaatta tttgggcgcc
     7561 tcgaccggct tgaaattcca aacagttgat aggggtgcca acaggaatat tttttagagg
     7621 taaacgatta ccttctttga tgggagcttt gggggaagaa acaacagaat cccctacttt
     7681 tagattggcc gtagctaaaa tataggattt agaaccgtct tcgtagctaa gataggcaat
     7741 attggcactg cgattggggt cgtattcaat ggcaatgact ttggctttga catcaatctt
     7801 atttcttttc cagtcaacag gacgaaggaa acgtttatgc tcgccaccgc gatggcggac
     7861 ggaaacgtga ccttgagagt cacgaccgct gcgttttttc ttgatagtta ataatctatt
     7921 tttcattttt tagggttaaa agatcaattt tttgatcttt tttaatagta ataacggctt
     7981 ttttacgatc gggatgaaca ataggactgc gggttttcca gttagttttt actttgcctt
     8041 taatattaaa gagattaact tttagggggt ttattccaaa aagtgattta aattcggtgg
     8101 caacctgatt tttattggct tttcgatcaa cgagaaagga gtacttccca attgactggg
     8161 cagcgatggc tttttcggtg actaaaggcc ttttaataat catattttta tttgtttaat
     8221 tgttttaagg cggtggccgt aaaaattaaa taattttgtc tcgataaatc ataactattg
     8281 agagatttaa ctgaaattaa tttgatgtcg ggaatgtttc caaaagcgcg ctggacgcta
     8341 tcaaaatgac gactggttat gataccaatt ttacggctat ttgccaatga ttcgatttgt
     8401 ttttcaagtt tgccaataaa ggctgaggca gattttgttt tggggtcaat cgtcgataat
     8461 ttgtcaatga caataattga tttatctttt gcaaatttag ataagaccga ttttagggct
     8521 gattgacgta gtttttgaga gagacgcatc ctaggttttt gattaccgcg aggaccatga
     8581 gcggaaccgc caccgacaaa ttgaggagcc ttggcggtac tatgtcgggc ttgaccagta
     8641 cctttttggg aatacatttt cttagtggta ccagcaattt caccccgatg tttggcgatt
     8701 gcatgggcgc cgcgctgatt gtgaagataa attttaattg cctgggcaat taaatcgtca
     8761 ttaattttag cttcaaaaaa agaggcagga agggtaatct cttttatttt ttctgcttga
     8821 agattgtaaa gtgtcgcctt catatgttta ttttttagaa ataacaaccc aagaattgat
     8881 atggcctgga acggcaccgg tgacgtaaag taaattttgg tctttatcta ccttaaagat
     8941 ttttaaattg gagactgtaa cctttgtatt accataatga ccaggcattt ttttaccctt
     9001 aataactttt ccgggagttt gggcgccgat agaaccagga gccctaactc ggtctgattg
     9061 accaccggta acgggctgac gatgaaaacc ccagcgttta atcacaccgg caaacccacg
     9121 acctttgctt cgactagaag cagaaacctg gtcaccggga ttaaaaacag tatcaatagt
     9181 gatttctgtc tttagttcgg gaacagtttc gccagtagat ttaaattcga ttaatttatt
     9241 tttgtgagtt ttgagctgaa gagcctcgta accatctttg tctttagttc gaagccggtt
     9301 gacagtgaga ggtttggctt ccaaaacggt gacaccaatt cttttgccat cctcggtaaa
     9361 aagactagtc atgtattttt tttgagtgag aaatccggaa atcatagcgg tattacatct
     9421 tgacttcaac ttcaactcca gagggtaggt cgaggtgctg aagactatcg acagtccgaa
     9481 gattagtgct gaagatatcg ataagacgct tatgagtcaa aacttcgtag tggtcacgag
     9541 cgtttttgtc ggtatgagga ctagttaaaa ctgtaatgag cttacgggca gtaggtagag
     9601 ggattggacc ttgaacagag gcaccggccg taatcgcagc ttccacaatc ttgaccgccg
     9661 cctcatcaat gaggcggtgg tcaaaagatt ttaatctgat acgaatccga gtacctttca
     9721 tctttattta ataatttcgg tgatagcacc agcaccaacg gttagaccgc cttcgcgaat
     9781 agcgaaacgg aaaccggatt ccatggcaac aggttttatt aatttaatgg taacattggc
     9841 attatcgcct ggcataacca tttcaacacc atcggggaga ataacctcac cggtaacatc
     9901 agtggttcta ataaagacct ggggtcgata accggcaaaa attggagtgt gacgaccacc
     9961 ttcttctttt ttaagtaaca aaacttcagc tttgaactca gtgtgaggag tgatggtgcc
    10021 gattttagca agaacctggc cacgttcgat atcatctttt tcaatgcctc gaagaagtaa
    10081 acctacattg tcgccagctt gaccttgatc aagttgcttg tggaacattt cgactccagt
    10141 aacgacgact tttttggtgt ctcgaagacc aacgatttcg acttcgtcat tgattttgac
    10201 gagaccacgt tcgattctac cagtagcgac agtaccgcga cctttgatag agaaaacgtc
    10261 ttcaattggc attaaaaaag gcttgtcaag ttcacgaaca ggctcgggaa tataagtatc
    10321 aagagcttcc attaattttt taatagattc aatgccttca gcatcaccgt ttaaggcttt
    10381 aagggctgaa ccacgaatga tcggaatttc actgccagga taaccatatt tggttaaaag
    10441 atccctaatt tccatttcaa ccagatctaa aaattcagca tcagtgacaa gatcgacctt
    10501 gtttagaaag acgacgaggg caggaacgtt tacctgcttc gcgagcaaaa cgtgttcacg
    10561 ggtttgaggc atgggaccgt cgggagcgga aacgacaaga atagcgccgt ccatttgagc
    10621 ggcaccggta atcatgtttt tgacataatc agcatggcct ggacagtcga tgtgagcata
    10681 atgtcttttt tcggtctcat attcaacgtg agaaatagca atagtaacaa ttttgttggc
    10741 atcacgaaca gtaccgccct tggcgatatc ggcgtatgat tttgcattac cctgaatttt
    10801 tgtaatagcc gctgttaagg tagtttttcc atggtctaca tgaccaatag taccgacatt
    10861 aacgtgcggt ttgtttcttt gatagacgtc tgccatatgt ttattttttg taaaaaatcc
    10921 gctttccgcg gaaaattaat aacaatataa ttgtagcgaa aaacgcttta gaattgtatc
    10981 agtaagacct cagattcgca agatggaagt gaagccaaaa agacagtatt aaagcttttt
    11041 cgaataattc atccgatgat agcataaaaa caagtcaatt aaaaccctgc caggtggcgg
    11101 gggtaaataa ttttttattt taatagagat tattcttttt tatcagtagt tttgatggtc
    11161 agtttttcct gaatatcgcg gggaacggga tcataatgac tgggctccat ataaaatgaa
    11221 ccacgacctt gggttaagga acgaatcacg gtggcatagc cacgaacagc ctcgaggggt
    11281 acagtagcag cgacggtggt aaaaccaaaa gcttcagtgg taccgccaac ttgaccgcgc
    11341 cgggaagaaa gatcaccgat aacagtgccc agaaattctg agggggtagc cacttcaaat
    11401 ttcataatag gttcaagaat cagaggatta gcctggataa aagcgtcttt aatggcatag
    11461 gatccagcta atttaaaagc ggcctcagac gaatcgacgt cgtgatatga accatcataa
    11521 acagaaactt tgatatcagt acaaggataa ccagcaatga tccccttggt taaagtttcg
    11581 cgaacacctt tttcaatagc cggaataaaa tttgcgggaa tgactccacc cttaatagca
    11641 ttaacaaact ccactccctc gcctctgttt ttaggctcga tgcgaatttt acagtgaccg
    11701 tattgaccat gacctccgga ctgatgaatg tatttaccct caccttcggc attagcagaa
    11761 atagtctccc gataggcaac ttggggggct ccagttttaa cattgactcc aaattctctt
    11821 ttaagacgat caacaataat ttcaaggtat agttctccca tgccggcaat aatggtttga
    11881 ccggtttcat gattatatga gactctaaaa gttggatctt cagaggataa acgattaagt
    11941 gccgctccca ttttttcctg atcatcggca gtttccggct caatactcaa agagataaca
    12001 ggttcggaaa attgaattgg ggagagggaa atagggtgaa gtttatcaca taaagtatca
    12061 ccagtagctg attcttttaa gccaatacag gcaataattt ctccagcata accttcttct
    12121 atcccctcgc gtttgtcagc gtgcattaaa agtaaacgac cgattctttc gcttttttgt
    12181 ttagttgagt tataaatctc ttgaccagtt ttgactgtgc cggaataaat ccgaacatag
    12241 gacaaagtcc caacgtgagg atccgattga accttaaaaa gaagaccgga aaagggctct
    12301 tgattttccg actttcgact gattacttca tttgtgacaa catcaaaacc ctgagtgggg
    12361 ggaatatctg atggtgaagg tagataatca ataatggcgt ctaggagagg gtgaataccc
    12421 ttatttctcc aagaagcacc gcaataaact gggaataatt ttcggttgat agtggcgtga
    12481 cgaagtaccg cttttaattc ggcgacagat atttcctgat caccgagaaa tttttccatt
    12541 aaagcttcat cctctccggc aattttttca accatagccg cccgagcagc agtagccgtt
    12601 tccaaatatt ctggagtaac atctttgacc gtaaattcca tgccttcttc gtctttgtca
    12661 tagacaatca ttttcatttc cataaggtca atcaccccca taaaatcatg ttcctcgcca
    12721 atgggataaa ccacgggtac aatgggagcg tgaagttttt gtttgatgga ctgaagtgtg
    12781 gcctcaaatg aagctccaat tttatccatt ttattgacat aagctagacg aggaacctcg
    12841 tatttatcgg cttgacgcca gacggtttca ctttgagcct gaacaccggc attaccatca
    12901 aagatcataa tggccccgtc aagaacacgg agagatctct ccacctcagc ggtaaagtcg
    12961 acgtgcccgg gagtatcaat aatattgatg cgataatttt tccaaaaagt agtgacacag
    13021 gcagattgaa tggtaatccc ccgctcccgc tcctgaatca tggaatcggt agtcgtggtc
    13081 ccctcatcga cagaaccaat tttgtggata tttccggtgt aaaaaagcaa acgctcagta
    13141 gtagtggttt tgccaccatc aatgtgagca ataatgccga tatttctgac tttatccatc
    13201 ggaacaattc tattttttgt gacttgaata gcttctgcca taatttaatt ttataaattg
    13261 attttaaaca tagtatcata gcataaatga agtcaaaaat attagtggta ggtggaggaa
    13321 ttactggact tgtagccgct tatcggctgt tgcaaaaaaa atatcaagta acgatatttg
    13381 aaaaaagtgg agatttgggc ggacttttag gcagttttaa aatagaggga actaatttag
    13441 aaaaagccta tcatcatatt tttaaaggcg acaccgaaat aatttcttta attaaagaac
    13501 tcggactcga aaacaaatta aagtggtatg agagtaaaac tgctatatat tacgacagaa
    13561 ctatttatcc atttgctgga gctatggatt tactaaaatt taaaccacta aatttagttg
    13621 ataaattgcg acttggcttt gtcaaaatat atttagaaaa agaaaataac tggcataaat
    13681 ttgaaaatgt tttggcttat gagtggatga agaagtggtg cggagaaaga gcttatcgaa
    13741 tagtctggga accgctactt aagggtaaat ttagcgatag atataaggat atttcgatgg
    13801 cttggatgtg ggcgagaatc cataccagag gaaattcgag cgagaaggga aaagaacttc
    13861 tcggatatat ggaaggcgga tttcaactga ttattgatga actagagaag agaataatag
    13921 agttgggagg aaagatagaa acgagaaacg agatttttga ttttagaaaa ctagaggaag
    13981 aatatgacaa gataattagt tcggcaccaa tagaaaatgt agagtattta ggggcaataa
    14041 cggtagtttt ttcttcagag caaaatttaa gtaaatatta ttggcataat atcaacgatt
    14101 ccaaatctcc ttttctggct tttattcaac atactaattt agtggggaca gaaaattata
    14161 gtagaaaaaa tatttattat ctggggacat atttgcccca aaatcataaa tattttacgg
    14221 ataaagacaa cttaattgaa aaagattttt ttgattattt aaagaaaata tttccggatt
    14281 ttagagaaaa tgaaatcgaa gaaaaatttg tttttaaatt caaatatgca caacacattg
    14341 taaccaccaa ctatcaatct tcaatctaca atcttcaatc aaataaaaaa attatccatt
    14401 ctaattttgc catgatttat ccggaagatc ggggggttaa ctttgcagtg agggaaggag
    14461 agaaagctgc cgctttagtt cctgaagacc agccgtaata tttcgaggaa aaaaaccaga
    14521 ttcaaaagaa aatttttgat tagataaact taaatttttg gcataaggac gggcatttgg
    14581 agtttgtaaa taatcttcaa gagatgagct tttgaccaag ttggcattta agtcaaaaac
    14641 ttgagcaata tttttggccg tgtcgtaggg ggattgagag gtgggtccaa ccagatgata
    14701 aatacccagt ggttttttat ccataaaaaa gcgtaaacca agaccaatgt catcaataaa
    14761 ggtgggagtt gtcttctggt cggtaaacat agtcaccggt ttattatttt gtaacttatt
    14821 taatattttt ctaacaaaat caaccttagt attcgataag gctcgataag gaaaggcaat
    14881 ccgaacaata gaagcgggaa gaccagaatc tataattaat ttttcggcga gatatttagt
    14941 ttctccgtac caatcaaggg gatggggagt atcttcttcg gtatatacag tttttttagt
    15001 accatcaaaa acaaaatcag tggaaatatg aattaaatgt ttacgatact ctttacacaa
    15061 atcgacaatg ttttgagtac cgtcgacatt taactgataa caaagaccgt ttttatcccc
    15121 tttttgattc caagcggcgt tggtatcggt aaaagcagca aaatgaataa tagtgctcgc
    15181 cccgggatta tttttgaaaa actgcttcag ctgtttttta tcaagaatat taacgccact
    15241 ctcaagggaa gcatcaacaa aatttaagtc cggatttaac tcaacgactc tggaaccaac
    15301 caggccggat aaaccagtac ctaaaatttg ttctttcata ataaatcctt tggggaaata
    15361 tctcttaaat ttggtagtat tttatcttta ccacttagta caatttcact acggctcagg
    15421 ggtaaattaa tgagaagatc gggatcaaaa acattaatcc ctctttcaga ttggggagaa
    15481 taaatcccat cagtaacaa
//
LOCUS       DHGB01000065            8884 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_41568, whole genome shotgun sequence.
ACCESSION   DHGB01000065 DHGB01000000
VERSION     DHGB01000065.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_41568"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         3016 a         1478 c         1796 g         2592 t
ORIGIN      
        1 cggaaaaatc gacagaaaga cgaaaacccc aaaagataat agaaaagatg gaggctaaaa
       61 tcaaaagaga agaaataaac aaaaatagtg gcctaaattt cataatattc atattttttt
      121 cctggctaaa acccttaata aattgcgggt gacaaagact ccggtaaaca ttcccaaaac
      181 tattccgaga agtaaagtca ccgcaaaacc gcgaaccatg cccgagctgt tgagaaaagg
      241 ccaattaaaa ggattaaata aaatcagggc agtaatgata gtacaagtat tggcatcttt
      301 aatggaattc caggcccggc cgaaactaat ttccagagcg ttgttccatg atttaccggc
      361 ccgaagttct tctttcatcc tttcaaaaat taaaatattg ctatccacgg ccatgccgac
      421 agataaaata aaaccgacaa tacctggaaa agtcaaggta attgggataa gacgataaag
      481 agtcaaagta atgagtccat aaataataag accaaaatcg gcaattaagc ctaaaaatcc
      541 atagttacca atcataaaga aagaaacgag gacgaggcca actagcccag ctttaatccc
      601 tttgttaata gaatcctgac caagagaggc acctacctga gactgctgga ttaattttat
      661 cggtaagctg agggcgccgg cattaagttg ggcggaaaga aatttagctg atttaatgtc
      721 aaagttgccg ttaattaccg ccttaccatc gggaatgata acccctacct ggggtgcagt
      781 aataatctca tcatcgagaa gaatggcaag tcttttattt aaataatcag tagtagcttt
      841 ttcaaaaagc ttggcgcctt cagaattaaa ttccaaagag acttcgatag caccagtatt
      901 gggattgggt tgaggcaaag cttttaaaag atgagagcca ttgagaccgg tatcaactag
      961 aatatcgttt aaagtcgcag tggcagtagc ctcgggaggg acatcaataa tacccttaaa
     1021 atttaactga gcagtttggc caattagttt tatagcttcg ttaatatcgg taactccggg
     1081 aagatcaact gtcaaacgat gattttcgcc ctgacttgaa agctgaaccg tggcctcgga
     1141 aactccaaat aaattaaccc gacgttcaat gttagttttt agcgactcga gcgcctcggg
     1201 taaattatct ttactggtct ttgaagtatc aatttcaaat aaaagcgagg ctccaccggc
     1261 cagatcgagg ccataacgaa gatctaaatt tggtttaaac tgaaatgaac cgagattaaa
     1321 agaaaaatta ggacgataaa aagtacggcc gagtagagta aattttgggg gcaagttgat
     1381 gatcagacac aaaaaggtag cggcaataat cgaccaaaag atagtaagag tctggcgatt
     1441 atttttcagc ataaccaaga gagagttgac ggggatgacc gctaaaagta taagtaattg
     1501 aagttttgga agaagaaatt ttatactgag ccaaatcccc ttctttaata tcaaaagatt
     1561 taatgatttt attggggata gtcaaagcca gagaatttcc ggttttaatg acttttcgat
     1621 ttttatccat ttagttttcg agaagagcag ggttaccaat tataacaaag ggtgaagaaa
     1681 ggaagaaatc aacaataaag ggatctcttt tgctttgacg aagcttgagt tccggataat
     1741 ccataaccat ataattaatt tcaacatcgc gttttttttc ttcagcagta atagcatttt
     1801 caacagtccg aatcggtaaa tcaccgacaa taattaaatc gatagagtcg gggcgggctt
     1861 tttgccataa aagaaaatga taggaaacta aaagaagcct cgctttattg agcttttccg
     1921 ataaatcaga atttcgataa gcaagtaaga ggagtgaatt aaatagatga tgatggggat
     1981 tgggagtgta ataaaggcga ttgccccgac tttcggaaag taaaatattg acctttttga
     2041 gactggcaag ctcacgacga actgaattaa tttcttcacc agaaagacga accagctgac
     2101 gaacataaaa aagttctctg ggattttcaa aaaagataca gattaattta cttctagttt
     2161 tggaagttaa aatggtgtta agagaggttt caaccttcat agctaatttt attgcccaga
     2221 ggcaatattt ctatccagat ttgaccggga acaaaattaa tttctttacc actcgtttta
     2281 tctttaaata tagttcgggc ttgacgatca gccttgctcc aggtgatttc ggttttttga
     2341 ccattgctaa aataaagacc agtaccagag ccaacaacag cgtaaagatt gtgggcatgt
     2401 tcatcgatag aacgtgattc tttgacaaac tgaataatta agtttttagt actaagctgc
     2461 tgttgattat taaaatcaac gaccgcttca ccgccattga ttctcagata acgatttgag
     2521 atggcatcat atttccaggt gacggcgtag gccttgtccc cccagaaatc aaaactgatg
     2581 gtattaaccg tagtaccgac agatttatct tcggcattaa aaagccaggg gcggaatttt
     2641 ttgtcccagg aactattatt tatttcggta agattcgtta aaccacgact agcagctaca
     2701 ttccacaact cagtggtgga acagtacatg gtgtgttcag tgtccctggt ttcgccggta
     2761 cgatccggtt cacggcgaca ggctttataa gagagggaaa attgggataa atcactccag
     2821 gtacctttgt tgttccagcc atatttagaa atttgttcaa tagcctgggc cttaacactg
     2881 gtcgtacatg gaccatcggg ggtagcggct gaacagttgg ctccgccaac atggacgtaa
     2941 agtggataat cggcgtattc agaggctaag tcaagaaaat aggtccgggc ggagcggaca
     3001 ggaccaacat catatttttg agttgaagtt tgagttgcat tacaataaaa aactcccata
     3061 aacctggtaa ttcctccttc agcaatagct tcgtaaacta catctgcatt catcagacct
     3121 gattgaggtc gagaatcaag gtgattttca atcatcacgg ttaagggacg acgagtggac
     3181 catttttctt tagcttcaac agaaaatttt tctccgttga taggacaaat ttctgttttg
     3241 ggaccttcaa aaaccaggta accggtttgg gcctgagcca tgggtgtagg ggtaagggaa
     3301 gtagttttag ggctactttt gaccggagcg agtgaagcaa ataacatata ggaagcgaag
     3361 gtgccaacta cgaaaacgag agagtaaaga cctatcttta aaatttttga attcataaaa
     3421 aattaactat taatagcttt taatatagcc gatttttctt cttttgacaa agttatatta
     3481 tcaccctgac cgtttttaat gggcttggca taaatccgag taaaagaacg gtggtgaaga
     3541 gaagtaagta aaatgccgga attttccttg tttaatagca ctaaaataaa actttgatcg
     3601 ccaccgacat catcaaaagg gttgaaacga actagtttta ttttttcgat acgaccagcc
     3661 gtctctgaaa tttgcttatt ttctggtgat aagtgacgaa atttggttaa gattaaataa
     3721 agaagataga taaaaccgag aaaattaata atggtaagag tcaataaagt attttgagac
     3781 atgatttaag ataattttaa caaaataggc catgttatac tgaagttgaa tgaaagaagc
     3841 gaaaatggaa aaaaatgaat atagccagta tttacgtaaa gatttattta aaagcttttg
     3901 gcttagtttg gtgactattg caatcctggt agctttgtat tatgtttgga agtgagttag
     3961 ctttaattat tacaaggagg tgaacaatta atggcagtta ttaaatttta ttctgtaaag
     4021 tcaagaaagt ccgtagaagt agacgtttct aaagtcaaga aagttatgct taaaaacggc
     4081 agaccagcag ctgaagcggt tgatcctgaa actggaacaa aaatgttcaa aatcctatcc
     4141 gcagctgatg cagctttgtt ataagtagtt ttttggtaga atatattact tcaaagaggt
     4201 tgaagggctt aatcgagacc gcctgcgcct aacggctacg gcgagtcgag gtaggaaagt
     4261 ccggaccaca aataacacgg aacctaaata attagggtaa ggcgtaagct ttaggctctg
     4321 ctacagagac gaataggtat cctacgccgt aaggctacgg aatactgaag tgaaacgagt
     4381 aaccttccgg tggaaatctc aaatcgaagc gttaatatct ggttattccg ccataggcgg
     4441 acaagtgaga gcttcaggta gagagaatag atagatggcc cttaaacaga aaccggctta
     4501 acaatctctt tgaagtctag tttttatctg tatttagggc tcggtaaatg gtctcgacgg
     4561 taatatagag agggagagca ataattgcac caacaacgcc gccaagcctg gcaccagaag
     4621 caattaaaag gatggtaatt aagggattaa tcccgattgc ctctttcatt attttgggga
     4681 caataaaatt gttttcgagc tgctgaacga ttattcccca gatgacggca aggacaccag
     4741 taatcggcga gatagtcaaa ccaaaaatac cagctaaaat agaagcaatg gtcggtccaa
     4801 tattgggaac aagctctaaa atagcggcaa taatagccag aggcagggca aaatttactc
     4861 cgatgatgac atagccaaaa taagagagaa tgccaataat caacattagc attagctggg
     4921 cactaaccca catactgaga cgtttttcaa gctgattaaa aacattaatt gcttttaatg
     4981 atttttgacc aaaaatattt ttaagatatt tgtcaaaatt tttccgttcg agaagtaggt
     5041 aaaaagtaat gacaaaaaag ataaaaatag aaagaatgtt ggagaaaatg gcaacaacaa
     5101 gagaagtaat attctcggga agattttcga gtattttaaa ctgagaggaa aaatcaacaa
     5161 aatttagacc taaaatatca atgtttttta aagccatcgg taaattttta attaaagttg
     5221 tcgactgctc aacaagatcg ggaatcacac cgacgacgga aaaactcaga atggcaataa
     5281 taaaaagata gacaataaaa atagctagag ccctgggcat ttttgtcttt tctagccagt
     5341 ttacgggggg gtgaagacct tccataaaga gaaaacaaag aaaaacagtt agtattaggg
     5401 cgcgaagctg ccaaagaaca agaagagaaa ttaaaaaaag aacagtgaaa ataattgtac
     5461 ggtgagtaat ttcaactttt tgagctttca taacagattg taccatttat taactttagt
     5521 taaaatttgt gataaatatt ttggtttcga aatatcaata aaaataatat ttttttgctt
     5581 tttaaaccag gtaagctggc gacgggcgta agcatgctcg tcaaaannaa atatggacga
     5641 aattctttat aggctaaagt attaaaaccg ggatccgaaa aagaatattt tttaagtaat
     5701 ttttttattt catttaaaag acctaattta aggcgttttt ggacacgaga gtctatacga
     5761 ggatagagaa aagaggaaga agcagtgaga gaaaggtgga gaatgtcata atcttcaatc
     5821 ttcaaactac aatcttcttt tttggcgaat gataatttaa tttcaatttt tctgattaat
     5881 cgatgggggt tgtgtttttc ggaattattt aaattatcaa aattcttttt atctaaaaaa
     5941 cgataaattt tttgaagagt cgcagtagat aatttattta aaaaatagcg taaaaattta
     6001 tttggtttta tggaataagt tgatttgttt gggttgacta tggcgtcaat atattgaccg
     6061 gtgccgccga caataattgg caatttattt tttttatgaa tttgcgagat tttttgaaaa
     6121 gctaattttt gatactgggc cacggaaaaa ctttgatcgg gggtaattaa atcaattaag
     6181 tgaattttaa tattttttgg ttgatctttg ccagtgccaa tgtttaaacc ccgataaatt
     6241 tgacgagaat cggcggaaat taactcgcca tgatattttt tggccagttt aatggctaaa
     6301 ccggttttac cagtagccgt gggaccagaa attactaata atttacaatc ttcaatcttc
     6361 aattttcaat tttgaatatt aaacattaaa tctaaactcg acaatgtcgt cggggtgcat
     6421 gatataggtt ttaccttcgt ggcgcagaag accttttgtt ttcgctcctt cccaaccgtt
     6481 attatcaaca aaatcattgt aagaaacaat ttcagccata ataaaaccat gttggaaatc
     6541 agtatgaatg acaccagctg cctgtggtgc cgtggctccc tgatttatag tccaggctct
     6601 gacctctttt atgccggcag tataaaaaga ctggagaccc agagtctcat aagctttttg
     6661 gataacccgt tctaaaccgg atttttccat acctagttct tttaaataat tttttttatc
     6721 ttcgtcagat aactcagata attcaaattc agttttggca caaactggaa gaacagaata
     6781 atcttcaatc ttcaaactac aatcttcaac atgctgataa gtattttcat caacattagc
     6841 gacgataaag tagggtttgg cggtgagaag aaaaaattcc tttaacaaaa ttttttcatc
     6901 gtcatccaaa tcaaggtgaa tagctaattt accctgttct aggcctgatt ttaatttttt
     6961 agctaaagca tattttttag tttttaattc gggggcattg tttttattgc cgtctaaata
     7021 tttatcaatg ctttccaaat ctttgataat taattcggcg caaataactt cgaaatcgcc
     7081 ctgaggatct acactcccct ctttgacgac actagggtca gaaaagtgac gcacgacatg
     7141 acagatggca tcacagtctc ggatattagt taaaaattta ttgcccagac cttccccagt
     7201 ggaagccccc ttaaccagac cggcaatatc cacaaattcg acaatagcgg ggataatttt
     7261 ggtggtttta acaatatcgg ccaagacctg taatttatcg tcggggactt caaccacacc
     7321 aacatttggg tcaatggtgc aaaaaggata attgctggcg tcagcaattt gacgaccaag
     7381 aagggcgtta aacaaagttg atttacccac attaggtagc ccgacaatgg cgatgcttaa
     7441 tttcatactt atggattata ccgtgaatag aaagaattgt tatttggtgt ttgtggacat
     7501 ttagatcaaa atcagagtca tttttttgat gtaaaatctg tttatgaatt atccaaaaca
     7561 tctatcggaa aaaattagag aagaagtggc ggtaagttat ttaaaacacg agttaaagaa
     7621 aatttttccc agcattgata aaaaagaaat tgaaaattta atgaataata aggaactttg
     7681 taagccacgt gacttttgga atggtttaca cgggatatct atgggtttca aattaaaaaa
     7741 tattttatat ttagtaactg cggaaaatat tatatgggaa aagagaacga tgaaagtaaa
     7801 ggaattagat tttggagtgg ggatgaagat tttaagttta gacgaaatca ttaaaattag
     7861 aggtaatgat tttgaaagag aaagtgatcc aataattgcg attgaaaaaa ttgagaatga
     7921 taaaaaggta attaaagtcc acgatggaaa tggacgccta gcaagatata ttgccgaaaa
     7981 taaagaagaa attgaagtat acttgggaaa aatggaagga aagtttcctc aaaatttctg
     8041 gttgccgacc agtttattaa tagataattt atattttgtc tatgaggcga ttaaaaatga
     8101 agacgaaaaa ttatttaatg atcaaataac ggtgattgaa gatatgttaa aaaattcaga
     8161 aagcggaaaa atagaattta gggaaagggc gttaagtaaa aaagaacctt attattcgaa
     8221 aattctagaa atgattaagg ggctttagtt tccagacttc gttttgaaac aataaaaaaa
     8281 gcccccacct aagtgagggc ttttgattgc ttcgttaaca ctcgcaatga cgcaggtatt
     8341 atttgaccat tttcttgact ttggtgccag gaatatagcg aggcatgcgg cgagccataa
     8401 ttttcttgga ttcttttgaa ccaatggctt taatggtttt agccttgaac attttggttt
     8461 tgaaagttcc aaaaccggaa agtttgacga cttcgccaga ggcaagagcc ttcataattt
     8521 catttaaaaa ggtttcaaca gaaaccttgg cagctttctt actgagttta gccttttgag
     8581 ctacgacttc gattaggtct tttttggtca ttttgttttg aatttataat ccagttatca
     8641 ttattagcta ctttccaata aatttcaagt attactcacc atttttaacc gcagatttat
     8701 tgattttgct accaacaata gtcgaagatg tcctaagttc tcggataact gtaactttta
     8761 tctgaccagc ccacttagcc tcttcctcta gtttatcggc aatattttgg ctaagtacgg
     8821 tggcttcttc atcgttaaca gccgaaggct cgacaataac catgacttca cgaccggctt
     8881 gata
//
LOCUS       DHGB01000066            3049 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_214248, whole genome shotgun sequence.
ACCESSION   DHGB01000066 DHGB01000000
VERSION     DHGB01000066.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_214248"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT         1135 a          458 c          643 g          813 t
ORIGIN      
        1 gtttgccatt atttttagaa taattattgg gagaagtaat taaaagtgaa tttgggggta
       61 aagttaaatc agcaatttcc caatcattga caaaaataaa tttatcaaaa gcgttgaccc
      121 agtaccaatc agcatgaaaa tcccaattta aattgggatc gttttgatat ttctcaggat
      181 cccaaggcca ataaaataag ataaattcat gggcttcgcc gtattttttg gttatataaa
      241 ttttttcgta gtttgaataa ttttctttta gtttggctac catttcctga tagccgtatt
      301 gccagttttg agaatagttt tgattgtatt ttaaatattt tgaccagtaa actaaaaatt
      361 ccagaagaaa tatccaaatt actaaataga aatatttatt tttcttgatt aagcttgccc
      421 catagcttat ggctaaaaaa tagaatggca aggcgatggt ggctcttatt gaaggaaagt
      481 cgccgacagt tagggcggcg ggaagcagag taatcagcag gggtataaaa aattgtcgaa
      541 gtctaaatag gccaaaataa aaaaggggta aaagaatcac aaaaagtaac ccggtcccgg
      601 gaggattaaa ttgataattt tgtgatcccg taaaaaataa ggggaaggga ttaaataaat
      661 ttagataatt gcccagcagc tgattagaaa aataaagaac tttattattt aaagcccgat
      721 ttaatagagc cggaccttca aagaggcgcc tcttttcatt gattaaatta atagaattgg
      781 gattgatgat acctacccaa cgatttctag cccgcgactc gggtgaaaat aaatttagaa
      841 gattgggaat ggccagaaat aaaaaaatga tgagggcgta aagatgagtt ctatttttta
      901 gctgacgcca ataataaata atgaaataga taataagcag ggggacggca atacgggtat
      961 tgtgatatga gtaaagtgaa attccaaaaa atagagccga aagataggcg ttatttggct
     1021 ttaatagtga tttataaaaa taatatagtc cggcaataaa aaagaactga gctaaatttg
     1081 actgaaagac gccgcgtgaa ggaaagaggg cccagggtga aagagcggct aaaaacatat
     1141 taaagagagc caatttttca tttttaaaaa ttaattttgc caaaaaataa ttaaagacga
     1201 caaaaagaaa gccaaatata gctgcgggta ggcgaatgga aaaagcattt agcccgaaaa
     1261 gagcgattga aggcagggtg aaatataaat tggcggcggt gggataatca ccgtaggctc
     1321 gaaagttgaa aataggccac gattgacccc actgatcttt tccggttttt agaagtgaat
     1381 aggcattgta accatgagaa atttcgtccc aatttaggcc cgaaggcaga gaagctaaat
     1441 tccaaaagcg aaaaaatcca gctaataaca gaattataat aaatattatt ttatgaacgt
     1501 ttttcacgga ttataagtat tataaagctc cccagtgcca gtagggaaaa aagattagca
     1561 aaagtacgaa tggtggtatt tttgaaggcc agttcataac tgtgcccccc ctgatctagc
     1621 tcaagttgaa ttaagccaag atttgggtct attttataat ctgatttttt cccatcaatg
     1681 actaaattcc aatttggaaa ataaactacg gggagattaa caattgcttt aggagttgaa
     1741 actgagaaat cggcctctga gaaatgacta tttttatagt attttataaa gtcaacttca
     1801 ccctcgagga cagtaggagt atggggggca tagtcttgag gaaaattttg accgtatttt
     1861 ggccaatagt ccataagacc ggcggcactt tggcgaatta attcactgcc acttaatttg
     1921 ccttgatcag ttaaatttgg ataccagata tcttctctga aatacgaaac gctggtgata
     1981 gtaataatta ccattaatgc ggtaaaaatt ggcaatttaa atttattttt tattaaatag
     2041 gccagtcccc cgcttataaa agaaaaacag aagatggtga gtcccagaaa tcgccaggga
     2101 aattggaaat aggtcataaa gggaaataac cgccaaataa atgtggattt gttgtgggtt
     2161 aaaaataagc ctaaaagtcc aattataaat aataaaataa ttaaaaggcg gtgttttgaa
     2221 tttttactca ataaataata taaaaaaaca agggtgggaa ctaaatattg aaataagccg
     2281 atgttaaacg aaagaccatc gataggaccc cacatggtgc cagagtaacc ccaaaaattt
     2341 gaaatgaata actgttttaa ggtggcaaaa tgaataatat aactgaaaaa tccctgagtc
     2401 atggtgttta ccgttgttaa atttttttca aaaaaagccg ggaggagaaa aaaactacat
     2461 atagctgatg accaaagtaa agttcgaaaa aaatacttaa atttcttaat caatatagaa
     2521 aatatggcca atagaggtat ggagtaaatt aaggttaaat tgtggctgag catcaaggag
     2581 ctaagaaata ataccgataa tttagttttg cgccgagaga tactccaaaa taccagaggc
     2641 aggagggcca gggcccaggt ttcgccgaga gcacctcgaa caaaggtatt gacagcctgg
     2701 tagggggcta aagaataaag aatgccggct aaaagtcccc caagtggacc aaagaaagca
     2761 gatgaaaaca gatacatgcc aaaagttctt aaaaaggctg ggattataaa aacaacttta
     2821 attgagttaa taaaatttaa accgagtaga tgaaaggatt cggccacagc atagggcaga
     2881 ggtgaataaa aattatagag tggatagccg tagcccaatc ccccattggc gatatatcga
     2941 caagggattt ggccatcttt taaacactgg tcaaactgtt gtaaacgaaa tatttgaata
     3001 tcatcctgca tggagggata accggggcga aaaaggcgcc aggaagaaa
//
LOCUS       DHGB01000067            3288 bp    DNA     linear   ENV 28-SEP-2017
DEFINITION  MAG TPA_asm: Candidatus Shapirobacteria bacterium UBA4809
            UBA4809_contig_103843, whole genome shotgun sequence.
ACCESSION   DHGB01000067 DHGB01000000
VERSION     DHGB01000067.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06453303
            Sequence Read Archive: SRR5324124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Shapirobacteria bacterium UBA4809 (hydrocarbon
            metagenome)
  ORGANISM  Candidatus Shapirobacteria bacterium UBA4809
            Bacteria; Candidatus Shapironibacteriota.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2017) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 22.24x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Shapirobacteria bacterium UBA4809"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA4809_contig_103843"
                     /isolate="UBA4809"
                     /isolation_source="Suncor tailing pond sample"
                     /db_xref="taxon:1953385"
                     /environmental_sample
                     /geo_loc_name="Canada: North Alberta"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX559915 metagenome;
                     metagenomic"
BASE COUNT          983 a          491 c          805 g         1007 t
ORIGIN      
        1 atggtgattt tacttttgta ttcaatgcct ttaaaaatca ttttattggc ggctcttttt
       61 tcaggtttgg taaaaacatc attttcccat tcgtagccat tgatataaag agactcttta
      121 aaggggatcg aaaattcttc aagatagtag ctttgcatgg tatcgcgaaa gatggttttg
      181 gcgtactctg gggggtaatt aaggcgaatt aaaattggac tggtgtaggc tgagcggtaa
      241 aaattgatga cctcggtgcg gctaagatta gtataaaagg cggtcacatt tttcatttga
      301 accgtatctc ctggttcatc agacttaacg ctatttggta agtcttttaa gactggggtg
      361 ggaattagaa gataagcgag aaaaaaggca ttaactaaaa caactaagat ggggaatatt
      421 tttttaaaca ttattgaaaa agcatgggcc ggcctaattt atattgagga tctttgagct
      481 ggcgatcgga tatttttttg gcgggaattc caccccaaac ttcgaaatcc gggacatttt
      541 tggtaacgac ggccccggcn nggaccaata aaaacaaaat ctttaatggt gacgggggca
      601 aaggaattgc cgaaatctgg ggaatttata tcgtgctcat ccgtataaat taagacctcc
      661 gaggcaatat cgacatgaga gccaatggtt agtccgcccc ggccatcaag aaaggtgcca
      721 tagccaataa tagaattttc cccaatacta atgccactcg gtttaaaaaa attagccccg
      781 aggtgaattg tgctgctaaa gggcatttta attccggaaa ttaaataaaa gatttttcga
      841 ataaaatgaa tggggataaa accaacaact ctcaaaatta agagccaaaa ttcagaaaaa
      901 atggtcagga ggcggttgaa aattttgggg atgaggtttt ggggggtaat tttttggccg
      961 acatattgat tcataaatga attataccct gtcgattata aaagttgatg aaagagtaaa
     1021 gacaaggatt cggctgactt tttccaagta aattttgctg attgaagttg acctttgtta
     1081 attaattttt gccgaagatc ggggtctaat attttttgaa aagcggcgat aagactcccg
     1141 gtgtcagtgg gatcgatata aagagcgctg tcttttaaga tttctttgag ggggggaatt
     1201 gaactggcga tgaccggggt acccacaatt tgactctcaa tggcgggaat accaaaacct
     1261 tcgtaagtac tggggataac taaggctata gctccatggt aaagagccca tttctcctct
     1321 tcgctgatga aatcggtaaa aactacttca tgggttaggc ctagtttttg gagaatgatt
     1381 ttaatttcat caaataacca gcccttttta ccggcaatta ctaattggaa aggaagagct
     1441 ttagagcttt ggagctttag aaaatgagaa aaggctttga tgaggaaggg ataattttta
     1501 ttgggcttta aagtgccgag ggccaaaaaa taaggagaat ttattttgaa tttggctttt
     1561 actttttgaa gagtgagttt agatattttg ggaggagcag aaacgccatt gtaaacgatt
     1621 gaaatttttt ccggaggaat ttgataggtt ttgatgagtt cgtttttggt aaactgggaa
     1681 actgccacaa tatgttttgc cctatctatt gatgatttag tccagtttat cagttgatat
     1741 aaatcttttt tggtaaactc tttttgaaaa tttaaatagc cgatatcgtg gatggtggga
     1801 atggtgggga ctgaggaaaa agagggtgaa tagtgaccgg gtgagtataa aagttttatt
     1861 tttggtgaag aaaataaaag gtggagaggt agggcaaact tggtccataa aaattttggc
     1921 ccaaaaactt gataatgcca gtaattattt tcagaaggta gatcacttag aggcttatct
     1981 tttagataga tataataatg atcgtctttg ttttgtttat aaagttccca taaaatatgg
     2041 taggcatatt ggccgactcc gactcggatt tttgtattgg cttcattgcc atcgattccg
     2101 attagcatta ttttagttta ctatagatct ctaaagtctt gatagcagtc aggtcccagg
     2161 aaaatttggc cgcggccgcc ctggcattct ctgataattg ttttcggaga gaggctgagg
     2221 agagaagttt atttatgcct tgaaaaatac tgtcaatcga gcgggggttg acgagaagtc
     2281 cggatttacc cacaatttcg ggcattgagg agatattgct agtgaggact gggacaccaa
     2341 ctttcattga tttgatgacc gggaggccaa agccttcgta aagagagggg taagcgagaa
     2401 agagagcgga agcgtgaagg gcgaccatgt ctttttcggg aatatagccg aggattttaa
     2461 tgcacggttt tttgaggtga aggacgtctt tgccccagcc atatttgccg gtaatagcca
     2521 gctcgaccgg agaatgaggg tgtttttggt gatagagatt aaaggcctgg ataaggcggg
     2581 ttaaattttt gcgcggttct ctggtacctt gggataaaat aaatttttta agaccgtatt
     2641 gtttttcaat gctggctttt ttgcgggttt gatactcggg actttgtttt aaaaagtggt
     2701 cgtatttatc ttcggcggct tcatagacaa ctgagcattt ttgggggtta attctgggaa
     2761 atatttttaa taaatctttt ttggtatttt cggaaacaca aataaaagac tgacactttt
     2821 tttgagccca aaacattttt tgacgatgag tcgagacgat ttggggatga agccagctgg
     2881 gattgataaa aggcgccagg tcatggacag tagtgacggc tttggcttta aatgtcggcg
     2941 gctgagtcca gtcagaacaa tgaaaaatat cgcagttacc gataaataat tctatgggaa
     3001 aaatatgtaa tttgttccaa agaaaggcta aaagtgattg aggcagatgt aaataataaa
     3061 tcttaacgtt ttggtgtttt tttaaagcgg aaatgtcttt ggggagaggt aaacgcatgg
     3121 aagaaaagaa gagtttatag gtattttgat ggtcgttttt taggagatgg cgaaccagat
     3181 taagggtata gttgctcacg ccggtgccgt aggcagtgga ggaaacgtca atgccaataa
     3241 tcataatatg tctaattgta tcagatacta agcttaagat attcccct
//
