LOCUS       DPUF01000001            3516 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_20923, whole
            genome shotgun sequence.
ACCESSION   DPUF01000001 DPUF01000000
VERSION     DPUF01000001.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_20923"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="DHV81_00005"
     CDS             complement(<1..533)
                     /locus_tag="DHV81_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCI92943.1"
                     /translation="MYADENVIKIKHAVLLEVAKAAFAGNLDEIRDDIPFTLIPGPTP
                     QFRCCIYKEREIIRQRVRLAEGKAPSANDDGNIIQVISSACEDCPISSYTVTENCQNC
                     LGKACINACKFGAIEAGRLRSHIDPQKCKECGRCAQACPYNAIAHLKRPCKFSCPVNA
                     ITYNEYGISVIDESKCI"
     gene            complement(774..2033)
                     /locus_tag="DHV81_00010"
     CDS             complement(774..2033)
                     /locus_tag="DHV81_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter permease"
                     /protein_id="HCI92944.1"
                     /translation="MLENIRLSFRGIWSHKMRSFLTMLGIIIGIAAIIAIVSTIQGTN
                     EQIEKNLIGSGSNNVKVTLQKDGYDMDLSYDTAPAGVTEVSAATLDSIKELDHVQNAA
                     LYRTRQASNAVYYLNTALSGGNITGVDDGYFDTASYVVMKGRGFSKNDYAKARRVALI
                     DESVESSLFSGESALGKTVEIQGYPFTVVGVVTEKDSYDLVINSMDDYYMYAYDDSQG
                     NVFVPSGTWPVIFGYDEPQNLLLKADSTDTMATVGKAAEEILNSNLNVTDGSDVTYKA
                     QDLQEQAKQIQQLSQSTNMMLIWIAGISLLVGGIGVMNIMLVSVTERTSEIGLKKAIG
                     AGKGAILGQFLTEAVVLTSLGGLVGVLVGVILSKVISMLNGTPTAISPAAAILAVLFS
                     MAIGIIFGMLPSYKAANLNPIDALRHE"
     gene            complement(2054..3516)
                     /locus_tag="DHV81_00015"
                     /pseudo
     CDS             complement(2054..3516)
                     /locus_tag="DHV81_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517397.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    3086..3131
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          823 a          889 c          680 g         1078 t
ORIGIN      
        1 ctgatacatt tgctctcatc aatcactgaa atgccgtact cattgtatgt aatggcattt
       61 acagggcatg agaacttaca tggtctcttt aaatgagcga tagcgttgta aggacatgcc
      121 tgtgcacagc gtccacactc cttacacttc tgtggatcga tatgtgagcg gagacgtcct
      181 gcctcaattg ctccgaactt acatgcattg atacaagcct tgccaagaca gttctgacag
      241 ttctcagtaa ctgtgtagct cgaaatcgga caatcctcac atgctgagct gattacctgg
      301 atgatattgc cgtcatcgtt tgcgcttggt gctttaccct ctgcaagtct gacacgttga
      361 cggatgattt ctctctcctt gtagatacag caacggaact gaggtgtcgg tcccggaatc
      421 agtgtgaatg ggatgtcatc tctgatctca tcgaggttac ctgcaaatgc agcctttgcg
      481 acctcaagca aaactgcatg tttgattttg attacgtttt cgtcagcgta catattatct
      541 ccttttcctt aaaataatgt atattttgtt acctaattaa ttacatactt agaagaatca
      601 ttataccatg tgacacttcc catatcacat gacctgattc ctctaaaaaa atgctcctag
      661 tatttatcta ctaggaacat tctacaaaat tttttaaact ttttctaccc ttgtattcat
      721 caaaatacaa agaattgtta tttttttcac aataacggca attttaataa aaattactca
      781 tggcgcagtg catcaatagg gttcaggttc gctgccttgt atgaaggaag cataccgaat
      841 atgattccga ttgccatgga aaaaagcacc gcaagtatag ctgctgccgg acttatcgct
      901 gtcggcgtgc cgtttagcat tgagatgacc ttagagagta tcactccgac cagcactccg
      961 accagacctc caaggcttgt gagcacaaca gcctctgtga ggaactgacc gaggattgct
     1021 ccctttcccg ctccgattgc ctttttaagt cctatttctg aggtacgttc tgtgactgac
     1081 acaagcatga tattcatgac accgatacca ccgactagta gtgatattcc ggctatccat
     1141 atgagcatca tattggttga ctggctgagc tgctgtatct gctttgcctg ctcctgtaaa
     1201 tcctgtgcct tgtaggtcac atcagagccg tctgtaacat ttaaattgct gttaagtatc
     1261 tcctcagcag cctttcccac tgtagccatg gtatccgtgc tgtctgcctt aagcaaaagg
     1321 ttctgcggct catcataacc aaatataacc ggccatgttc cgcttggcac aaatacattg
     1381 ccctggctgt catcataggc atacatatag taatcatcca ttgagtttat taccaggtca
     1441 tagctgtcct tttccgtgac tacaccgact actgtaaacg gatatccctg tatctctact
     1501 gtcttgccaa gcgcgctctc tcctgaaaac aggctgctct ccacgctctc atcgatgagt
     1561 gccaccctgc gggcctttgc atagtcattc tttgagaagc ctctgccctt catgactaca
     1621 tagcttgcag tatcgaaata tccgtcatcg acacctgtta tattgccgcc cgaaagcgcc
     1681 gtgttcagat aatagactgc attggaagcc tgtcttgtac ggtaaagagc tgcattttgc
     1741 acatggtcga gctcctttat gctgtcaagt gtggcagccg atacctcagt cactcccgcc
     1801 ggcgccgtat catatgatag atccatatca tagccatcct tctgcagtgt gacctttacg
     1861 ttattacttc ccgaacctat gaggttcttt tctatctgtt cgtttgtgcc ctgaattgtg
     1921 gaaacaatcg caataattgc tgcaattccg atgatgattc caagcatggt cagaaacgaa
     1981 cgcatcttat gtgaccagat tcccctaaag gacaatctaa tattttcaag catctttaat
     2041 cctccctgct aaactaatac ataccatctt ccgacacctt ggttttctca ccatccctga
     2101 ctccatacgg gaatgcgata tagtcatcat ttgtcagacc tgactttatc tccacatatg
     2161 agccccagag cgacttgcct gttttgacag actgcttttt gagctttccg ttgtcatcct
     2221 tgtatatata tgactgtccg ttctcggttc tgacatatga cttgtcgata tatagtccgc
     2281 tgtcagagcc ctccgatgat gtatctatgg tcaactcaag tgaatcgccc tttttgagat
     2341 tctgcggatt ttcaatgtat gcagtgtact catagaatga ggagtttgga ttgctgtctc
     2401 cataatagtt tacgctgtct gacggcacat tgcttatctc ggatatcgtg ccctcatagg
     2461 tatcacctgt catgtagttg actgctgata cgctctgtcc tacctttacc gtatccaaat
     2521 ccagctcgcc gattgttccg ctgatataca gtcctgatgc tcccgaaagc tgcaggaaag
     2581 gtgtaccgtc cttcggtgga ttggcaggat caccgactgt tgtcacaatt ccatccttct
     2641 ttgcatatac cacgccatcc tcaagctcgc tctgcagttc ctttaaatca gcctgtgcaa
     2701 ttctcttttg taaatcaaca tcggcaacac ttctggtctg ctcctttatg gcatccttaa
     2761 gctcatctgc tgtgtatgtc ttttcttctg ctgcggtatc agcttctgtc tgtgtggcct
     2821 gttctgtggc attgtctgct gcggcatctg acggctcatt tactgctgta tcagcagaag
     2881 catttcctgc tgtaattgaa cctgcaagga aaagctttat ctcatcgctg tcatcatagc
     2941 tgccaagctt atctcctctt acgataagct ccatatcagg tgcgttagca tctcctatta
     3001 caaaccgggc taccgcactc ttatcagcaa gtgtattcaa aagcgatccg gctacatatg
     3061 aagcctctgt gcatgtaaat acatannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nctctgtacc gcttccctta gcctctgaca aatccgatat cacatcatgc
     3181 tgttcatcag gcttttgctc aggtgcagcc ggctgtgtgt caggctgtgg ctcaggtgta
     3241 acaggagtct caggctgctt tgcaaccggc ttagtattct ttagcttatc aagcttctgc
     3301 ttttccgata caagctggtt ctcaagactc tgtatctcaa gctgcttcat ctcaaccgag
     3361 agtgaaagac tcgtcacatc atatgcaagg agcttatctc cggcctttac ctgctgatcc
     3421 tttgtgacat aaacctctgt caccttctgt ccatccttta aattgattgt ctgacatgca
     3481 tcgtcacaca ccattccctc actggtcatg gatgtg
//
LOCUS       DPUF01000002            8723 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_17556, whole
            genome shotgun sequence.
ACCESSION   DPUF01000002 DPUF01000000
VERSION     DPUF01000002.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_17556"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="DHV81_00020"
     CDS             complement(<1..677)
                     /locus_tag="DHV81_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine biosynthesis protein ArgJ"
                     /protein_id="HCI92945.1"
                     /translation="MSFTKVKKGVCAAKGFVANGLNCGLNSDKNKNDLALVYSETQCQ
                     AAAVYTTNKVKGAPIQVTKAHLEASGHTAKAVIANSKNANTCNADGVEKAQKMCELAA
                     AELSIDPNEVIVASTGVIGQVLPIEPIESHIKELAKGLSADGNDKAVNAIMTTDTVPK
                     QYAVKFMLGDCECTVGGMAKGSGMIHPNMATTLNFITSDVAISSELIQKALSEIVKVT
                     YNCLSIDG"
     gene            complement(767..2111)
                     /locus_tag="DHV81_00025"
                     /pseudo
     CDS             complement(767..2111)
                     /locus_tag="DHV81_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517035.1"
                     /note="catalyzes the formation of citrate from acetyl-CoA
                     and oxaloacetate; frameshifted; too many ambiguous
                     residues; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
     assembly_gap    1244..1272
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2160..3752)
                     /locus_tag="DHV81_00030"
     CDS             complement(2160..3752)
                     /locus_tag="DHV81_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase"
                     /protein_id="HCI92946.1"
                     /translation="MQFIVSKKEFIPPVIAPDIIETLGDDYEIWTYEDYERLPPLLVG
                     VYSYSAIPKCLAPLSTQGLVKSGVFRLQREFVPGLYGNGVYVVVIDTGVNYAQEAFMT
                     PEGKTKIAVLWDQNTDTVYSEDEINAAILSENPYESIPGDEDGHGTFVASVICGNDRP
                     QDDFAGVAPQAKLIVVKLKRAPQKLYDFYFIPDRKMVYSEVDVMRAVHFADEFAGQKG
                     APAVFCMALGCNNGSHTGAEDLSEYLDYITAKRGRAVVAAAGNEANSRHHYKGRITDT
                     VDDIEINVEQDMDGFYLECWALSPELFTLSVISPSGQSNPAGVPMRIDSGEYSFLLEG
                     TQVTIDYRQTGRQTRDLLIFIRFEKVVKGVWTIRVFPLSTIGGVFNMWLPMTGLLDAD
                     VSFIVSEPDTTLTMPSDAKLVITAGGYNSLNDAILLESGRGFDADGGIKPDLVAPAVD
                     ITGANLRGMYIALSGTSAAAAITAAVAALIMEWMIVRGNVLLANGVDIKNVMVRNCRR
                     KEKTDYPNKIFGYGFMNGFANF"
     gene            complement(3875..>4714)
                     /locus_tag="DHV81_00035"
     CDS             complement(3875..>4714)
                     /locus_tag="DHV81_00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI92947.1"
                     /translation="NEQIGKRAKTKADYEKAKASLDEYLSLYGYDSAAEYTQTLSQMK
                     KQLAEAGFAKENMDSALQKKTQFENDNDMDKLLAPNECPYTLDELNEMISKVEGSLDE
                     IRASLVQYGRQMDDLQEQLDMRDEKEQEYRNCKQIQAEEKHKYEILGLTSDYLTRARE
                     QFTARYMAPISNGFQKYFGILTENTDRDWQVDANISIRMKEQGQLRDVRTMSAGYKDL
                     IGICMRFALVDAMYPGEKPFLILDDPFVNLDDEKLARGRRLLAVLSQDYQVIYFTCHG
                     SRE"
     assembly_gap    4715..4829
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4830..6292)
                     /locus_tag="DHV81_00040"
     CDS             complement(<4830..6292)
                     /locus_tag="DHV81_00040"
                     /inference="COORDINATES: protein motif:HMM:PF13476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI92948.1"
                     /translation="MKLIACHINNFGKLSDLNINFNDGVNVINQPNGWGKSTLAAFLK
                     AMLYGFDTKKEPGAFERERKLYKPWQGGTYGGELDFEADGVEYRLVRTFGLTEKQDDF
                     HLYRLATMVECDDFSPLLGEELFDLDRSSFKRTIYIAQADCMFSSSDSISAKLGNMAE
                     NTNDINNFESATADIKDMMNKMSPNRITGSIKKRVNTLTALEQELKKYQAADEAAAQL
                     SEKIDEKTAQKKELTGIRAEYGRALQVASEDSRKQALRENYEQLCAVAKEKESIYNAA
                     MAVFGEKAPDEAELLEKTKQAGELESLKAVEDNLSFTPEEEQYLDTLSGMFKDGVPSD
                     DGLDEMQKKIANIQSVKNEYSHMELKLSQMTSLAKMADDEEEPETPEKTKLVPAGIVL
                     MVIGLLAAAVATVFSLNEKYNVKEIMFLVVGIAGVAMALCGVVMMVYGIRLNNKKQRA
                     YIRLMAEREENIKQKEIPIQELKEQLEQIQSGITSME"
     gene            complement(6296..7360)
                     /locus_tag="DHV81_00045"
     CDS             complement(6296..7360)
                     /locus_tag="DHV81_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCI92949.1"
                     /translation="MKIIHCADLHLDSKMTSNLSKEQAKERKMEILRTYSRMIEYAKN
                     NDVKAIIIAGDLFDTRNVSATARNLVKDSITSNPDIDFYYLKGNHDSDNFISKLDEIP
                     DNLKLFSNQWTSYSYGNITITGLEIDKSNQAAMYNSLVLDNDNYNVVTLHGQLGDEIS
                     TGDLKNKGIDYLALGHVHEYQSGQLDNRGMYCYSGCLEGRGFDECGQKGFVVLDIDDE
                     KLTAGFSFVPFAYRSLYTLYVDVTGAMTTQDVAVKMEKAISDSEYSSRSMVKFVLVGE
                     VDVDCEINTDFLKDMFEEYFYYEKVYDETRLLINYSEYEKDASLKGEFIRMVLGSDMT
                     EEQKSEVIRCGISALSGEEI"
     gene            complement(7482..>8723)
                     /locus_tag="DHV81_00050"
     CDS             complement(7482..>8723)
                     /locus_tag="DHV81_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCI92950.1"
                     /translation="NISMINLSDLTGGIPNSQKLKKKKPKEEKKVEPILDIHSIPAPH
                     RIKASLDDYVVGQEHAKKVMSVAVYNHYKRVMADNKHKAQEENTTAKQASNKYDGVEI
                     EKSNMLMIGPTGSGKTYLVKTLAKLLDVPLAITDATSLTEAGYIGDDIESVVSKLLAA
                     ADNDVERAEHGIIFIDEIDKLAKKRNANQRDVSGESVQQGMLKLLEGSEVEVPVGASS
                     KNAMVPMTTVDTRNILFICGGAFPGLEDIIKERLNKEASIGFKADLKDKYDGDENLLM
                     QATVDDIRKFGMIPEFIGRLPIMFSLEALTEDMLVKILKEPKNAIIKQYQKLLEMDEV
                     RLEFDDDALYAIAKMAKEKKVGARALRAIIEDFMLDIMYEIPKDDNIGTVTITKDYVE
                     KKGGPLIQMRGTPELADSQHV"
BASE COUNT         2136 a         2288 c         1553 g         2602 t
ORIGIN      
        1 ccgtcaatgc ttaagcagtt gtaggtaacc tttacaatct cgctcaaggc cttctggatg
       61 agctccgatg agatggcaac atctgatgtg ataaagttta atgtagtagc catgttcggg
      121 tgaatcatgc cgcttccctt tgccataccg cctactgtac actcacagtc tccaagcatg
      181 aacttcaccg catactgctt tggaacggta tctgtcgtca tgatagcgtt tacagccttg
      241 tcgttgccgt ctgccgaaag tccctttgca agctccttta tatggctctc aataggctca
      301 atcggaagca cctggccaat aactcctgta gatgccacga taacctcatt tgggtcaatc
      361 gaaagctctg ctgctgcaag ctcgcacatt ttctgtgcct tctccactcc atcagcattg
      421 caggtgttgg cgtttttgct gttggcaata acagcctttg ccgtatgtcc tgatgcctca
      481 aggtgtgcct ttgtcacctg aataggtgcg cccttcacct tgtttgttgt gtaaacagcc
      541 gctgcctgac actgcgtttc tgaataaact aaagccaggt catttttgtt tttatcactg
      601 tttaatccac agttcagtcc gtttgcaaca aagccctttg ctgcacagac tccctttttt
      661 acctttgtaa aactcatttg tataatacct cgattcttct aaatatgtct aatccccaac
      721 atatcgtagt attgatgtat gaatgatagc ctgtaacatc ctttcgttac tctatctcct
      781 tcatgacgct cttgtaagcc ggacggataa tcttgttcat gccgacgatt tcctcaagcc
      841 tgtgagcact ccatccaacg attcgcgcaa tggcaaaaag aggtgtgtaa agctccatag
      901 gaataccaag catctcataa acaaagccac tgtaaaaatc gacattagga gaaacaccct
      961 taaagatatg acgcttttca gcgataatct caggagccac cttctctatt gtattataga
     1021 gattcatctc cttatctcta ttctttgcca ccgaaagctt ctccacatag cctctgaata
     1081 tacgctccct cgggtcagaa agtgaataca cggcatgtcc cattccatag atgagcccct
     1141 tgtggtcaaa tacctcacca tccatcattt ttctgaggta ggctctcacc tcatcctcat
     1201 catcccagtc cttcacatgt gaacggatat cctgcatcat ctcnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nntgctttgg tcccttaagg gatgacatgg ctgccgcgat agcagagtat
     1321 gtatctgtac ctgccgatgt aaccacacgt gtggtaaatg tagagttgtt tccaccgccg
     1381 tgctccatat gaagaataag tgccatatca agcacccttg cttcaagctc tgtatattga
     1441 ttgtcagctc tgagcatcct aaggaagttt tcagctgtcg aaagctttgg atccggcctg
     1501 tgaatataca tgctctgatc attttcataa tgattatacg catgataacc gtaaactgca
     1561 agcatcggga atattgcaac aagtgataag cactgtctga tattgttgtc gatgcccgcc
     1621 tttgctacaa gaggatcata tgaagccagt gtcagtatac ttctcgtcat ggaattcatg
     1681 atatcctttg acggagcctt catgatgaca tccctcgtaa agttggtagg aagggttctg
     1741 cttcttccta aaacctctgt aaactcagcc aattcactct cagatggcag ctgcccgaaa
     1801 agcagcagat aagcaatttt ttcaaaacca aactcaccgg gccttaagtt atttgtaagt
     1861 gtctttacat tgtagcccct gtaccacaat tgtccgtcac atggcacgct gctgccatcc
     1921 tcgcccgttt taaatgaaac aatctccgaa atattcgtaa gacctgtcag aactcccttt
     1981 ccgtttacat ccctaagtcc tctgttgaca ccatattccc taaaaagact atctgaaatt
     2041 gtgtcatttt taatacagat atcctgtttt tttgcagcgt aatccgctaa taaattattg
     2101 ctttttgtca tccattcacc cttccttttt atcttttaga atatattata tccgttttct
     2161 tagaaattgg caaacccatt catgaatccg tagccaaaaa ttttatttgg gtagtcagtt
     2221 ttttcttttc tgcggcagtt tcttaccatg acatttttta tatccactcc gtttgcgagc
     2281 aaaacattgc ctctcactat catccactcc atgatgagag ccgccaccgc tgcagtgatt
     2341 gcagcagccg ccgatgtgcc acttagtgct atatacattc ctcttagatt tgcccctgtg
     2401 atatccacag ccggtgctac gagatccggc tttattccac cgtctgcgtc aaagcccctt
     2461 cccgactcta aaagtattgc atcatttagg gaattatagc ctcccgccgt tatcacaagc
     2521 ttagcatccg aaggcatggt cagcgtggta tcaggctccg agacaataaa gctcacatca
     2581 gcatcaagaa gtcctgtcat cgggagccac atattaaaaa cacctccaat agtactaagt
     2641 ggaaataccc tgatggtcca cactcccttc accacctttt caaagcgaat aaatataagc
     2701 aaatccctcg tctgtctgcc ggtctgcctg tagtcaatag tcacctgtgt tccttcaagc
     2761 agaaatgagt attcaccgct gtctattcgc attggtaccc ctgccggatt cgactgacca
     2821 ctcggtgata tgacagaaag cgtaaatagc tcaggtgaaa gtgcccagca ttccagataa
     2881 aagccatcca tatcctgctc aacatttatc tcaatatcat cgactgtatc tgttatgcgc
     2941 cctttataat gatgcctgct gttcgcctca ttgcccgctg ccgcaaccac agccctgcct
     3001 ctttttgccg tgatatagtc cagatactct gaaagatcct cagctcccgt gtggcttccg
     3061 ttattgcagc caagcgccat gcaaaagaca gccggtgctc ccttttgacc tgcaaactca
     3121 tctgcaaaat gcactgctct catcacatca acctccgagt acaccatttt acgatccgga
     3181 ataaagtaga aatcatacag cttttgaggt gcccttttca gcttcacgac aataagctta
     3241 gcctgtggtg ccacacctgc aaaatcatcc tgcggtctgt cattgccaca tatcacgctc
     3301 gccacaaacg taccatgccc atcctcatcg cccggtatgc tctcatatgg attttccgat
     3361 aaaatagcag catttatctc atcctcagaa taaaccgtat ccgtattctg atcccagagc
     3421 actgcaatct tcgtcttacc ctcaggtgtc ataaaagcct cctgcgcata attcacacct
     3481 gtgtcaataa ccaccacata cacaccattt ccgtatagtc ccggaacaaa ctccctttga
     3541 agcctgaaca caccgctctt tacaagtccc tgcgtggata aaggagcaag acactttgga
     3601 atagccgaat acgaataaac tcccaccaga agtggtggga gtctttcgta atcctcatat
     3661 gtccatatct cgtaatcatc gcccaatgtc tctatgatat caggagctat taccggtgga
     3721 atgaactcct tttttgatac tatgaactgc atattttacc tcatatgata taatatttag
     3781 atatgagggt caaaagatat tttgaccctc atataattat attctataaa taaaaatatg
     3841 ctaccataat ataaaacaaa gcttcgacca aaatttactc gcggcttcca tgacatgtaa
     3901 aataaatcac ctggtaatcc tgcgaaagca ccgcaagcag ccttctgcct cgagcaagct
     3961 tctcatcatc cagattcaca aacggatcgt caagtatgag gaacggcttc tcgcccggat
     4021 acatcgcatc cacaagcgca aaacgcatac atatgcctat caggtccttg taacctgccg
     4081 acatggttct cacatcgcga agctgcccct gctccttcat ccttattgaa atattggcat
     4141 ccacctgcca gtctctgtct gtattttctg tcaggatacc gaagtacttc tggaatccat
     4201 tggaaatagg tgccatgtat ctggcagtaa actgctccct tgccctggtc agatagtctg
     4261 atgtaagtcc aagtatctca tacttatgct tttcctcagc ctgtatctgc ttgcagttgc
     4321 gatactcctg ctccttttca tccctcatat caagctgctc ctgcagatca tccatctgtc
     4381 tgccgtactg cactaaggat gccctgattt catcgaggct gccttccacc tttgaaatca
     4441 tctcattgag ctcatcaagt gtatacggac actcatttgg tgcaagaagc ttgtccatat
     4501 cgttgtcatt ctcaaactgc gttttcttct gaagagcact gtccatattt tcctttgcaa
     4561 agccggcctc tgcgagctgc tttttcatct gtgaaagtgt ctgtgtatat tctgcagcac
     4621 tgtcatatcc atagagactt aaatactcat caagcgaagc ctttgccttc tcatagtcag
     4681 cctttgtctt ggcgcgcttt cctatctgct cgttnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gctccattga tgtaatgccg ctttgaatct
     4861 gctcaagctg ctcctttaat tcctgaatcg gtatctcctt ctgctttata ttttcctcac
     4921 gctcagccat aagcctgatg tatgctctct gctttttgtt gttcagacgg attccataga
     4981 ccatcatgac aacaccgcaa agcgccatag cgactccagc tatgcctacc acaagaaaca
     5041 tgatttcctt cacattgtac ttctcattca aactgaatac cgtggcaaca gctgctgcca
     5101 aaagaccgat aaccataagc acaatacctg caggtacaag cttagtcttc tcaggtgtct
     5161 ccggctcctc ctcatcatct gccatctttg caagtgaggt catctgcgag agcttgagct
     5221 ccatgtggct gtactcattc ttgacgctct ggatgtttgc tatcttcttt tgcatctcat
     5281 caagaccatc atcagatggc acaccatcct taaacatacc tgaaagtgta tctaaatact
     5341 gctcctcctc aggcgtaaag ctcagattat cttctacagc ctttaagctt tctagctctc
     5401 cggcctgctt tgtcttttcc aaaagctctg cctcatccgg tgctttttct ccaaagactg
     5461 ccattgctgc attgtaaatg ctctctttct cctttgcaac agcacaaagc tgctcgtagt
     5521 tttcacgaag tgcctgcttt ctgctgtcct cagatgccac ctgaagcgcc ctgccgtact
     5581 cggctctgat accggtaagc tccttcttct gggcagtttt ctcatctatt ttctctgaaa
     5641 gctgtgctgc cgcctcatcc gctgcctgat acttcttaag ctcctgttcc aaggctgtga
     5701 gtgtatttac acgctttttg atagagcctg tgatacggtt tggggacatc ttgttcatca
     5761 tgtccttaat gtccgctgtc gccgactcga agttgttgat gtcatttgta ttttccgcca
     5821 tattgccaag ctttgcgctg atgctgtctg atgacgaaaa catgcagtct gcctgcgcta
     5881 tgtatatcgt tctcttgaac gagctgcggt cgaggtcaaa aagctcctct ccaagcagtg
     5941 gtgaaaaatc atcacactca accatggttg caagcctgta caaatgaaaa tcatcctgct
     6001 tttctgtgag gccaaatgtc cttacaagtc tgtactccac tccatcggcc tcaaaatcaa
     6061 gctctccacc ataagtgccg ccctgccatg gcttatagag ctttctttcc ctctcaaagg
     6121 cgcccggctc ctttttcgta tcaaagccgt aaagcatagc cttcaaaaaa gccgcaagtg
     6181 tactctttcc ccagccgttt ggctggttta tgacattgac tccatcattg aaattgatat
     6241 taagatcgga cagctttccg aagttgttta tgtgacaagc tattaatttc aatttctaaa
     6301 tctcctcccc gctaagcgcc gatataccac acctgatgac ctcagacttc tgctcctcgg
     6361 tcatatctga gccaagcacc atccgtatga actcaccctt aagtgatgca tccttttcgt
     6421 attcactgta attgatgaga agtcttgtct catcatacac cttttcataa tagaaatatt
     6481 cctcgaacat atccttgagg aaatctgtgt ttatctcaca atccacgtcc acctcaccta
     6541 caagcacgaa ctttaccatg cttctcgatg aatattcact gtccgaaatg gccttttcca
     6601 tcttcactgc cacatcctgt gtggtcatgg ctcctgtcac atccacatac agcgtgtaga
     6661 ggctcctgta cgcaaacggt acaaagctaa agcctgccgt gagcttctca tcatctatat
     6721 caagcaccac aaagcccttt tgtccacact catcaaagcc tcttccctct aaacagccgc
     6781 tgtagcagta catgccacgg ttgtcaagct gtccgctctg atactcatgc acatgaccga
     6841 gcgcgagata atcaatgccc ttattcttaa ggtcgcctgt tgaaatctca tcaccgagct
     6901 gtccatggag agtgacaacg ttgtaattgt cattgtcgag cacaagagaa ttgtacatgg
     6961 cagcctggtt agacttgtct atctcaagac ctgttattgt gatatttcca tagctgtatg
     7021 aggtccactg attagaaaaa agcttaaggt tgtcagggat ttcatcgagc ttcgatatga
     7081 aattgtccga gtcgtgatta cccttaagat aatagaaatc gatatccgga tttgacgtga
     7141 tagaatcctt taccagattt ctggcagtcg ctgacacatt tctcgtgtca aataaatcgc
     7201 ctgctatgat aatcgccttt acatcgttat ttttggcgta ctctatcatc cttgaatatg
     7261 tacgcagtat ttccattttt ctctcttttg cctgctcctt tgacagattc gaggtcatct
     7321 tcgaatccag atgcaggtct gcacagtgga taattttcat ttttccaatt cctcttgata
     7381 tatgaacgtt tataaatcat tacagactac acatagattc attacgcata gccttgttgc
     7441 atcatttaca gccaattcac aatttcataa tcatttaact attaaacgtg ctgactgtct
     7501 gcaagctcag gtgtacctct catctgaatg agcgggccac ccttcttctc aacgtaatct
     7561 ttagtgattg taaccgtgcc gatattgtca tcctttggta tctcatacat gatatcgagc
     7621 atgaaatcct ctataatcgc acgaagagct ctggctccga ccttcttttc ctttgccatc
     7681 ttggcaatgg cataaagcgc atcatcgtcg aactcaagcc ttacctcatc catctcaagg
     7741 agcttctgat actgcttgat aatggcattc tttggctcct tcaatatctt tacaagcata
     7801 tcctcggtga gcgcctcaag tgagaacatg ataggaagtc gtccgataaa ctcaggaatc
     7861 attccgaatt tacggatatc atcaacagtt gcctgcataa gcaggttctc atccccatca
     7921 tacttgtcct tcaaatcagc cttgaaacct atagaagcct ccttgttcag gcgctccttt
     7981 atgatatcct caagtcccgg gaaagcacct ccgcatataa agagaatatt tctcgtgtca
     8041 actgtggtca tcggaaccat ggcattcttg ctgctggcac caaccggcac ctcaacctct
     8101 gagccctcta aaagcttaag cataccctgc tgcacggact ctccgctcac atcacgctgg
     8161 ttggcatttc tcttctttgc aagcttgtct atctcatcta taaaaatgat accgtgctca
     8221 gctctctcca cgtcattatc tgcagccgca agaagcttac ttaccacact ttcgatatcg
     8281 tcaccgatat atccggcctc agtaagtgat gtggcatctg ttatggcaag tggcacatct
     8341 aaaagctttg caagagtctt tacgagatat gtctttcctg aacctgtagg tccgatcatg
     8401 agcatatttg atttctcaat ctccacaccg tcatatttat ttgaagcctg ctttgcagtg
     8461 gtattctctt cctgcgcctt atgcttattg tcagccatga cacgcttgta gtggttatat
     8521 actgcaaccg acatgacctt ctttgcatgc tcctgaccta ccacataatc atcaagactc
     8581 gccttgatcc tatgcggtgc aggaattgag tgaatgtcaa gaatcggctc gacctttttc
     8641 tcttcctttg gttttttctt cttaagcttc tggctgtttg gtatgcctcc tgtcagatct
     8701 gacaaattta tcatgctgat att
//
LOCUS       DPUF01000003            3544 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_27405, whole
            genome shotgun sequence.
ACCESSION   DPUF01000003 DPUF01000000
VERSION     DPUF01000003.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_27405"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            1..479
                     /locus_tag="DHV81_00055"
                     /pseudo
     CDS             1..479
                     /locus_tag="DHV81_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019903.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
     assembly_gap    225..260
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            759..1811
                     /gene="recA"
                     /locus_tag="DHV81_00060"
     CDS             759..1811
                     /gene="recA"
                     /locus_tag="DHV81_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCI92951.1"
                     /translation="MAKEDKLKALDAAIAQIEKQYGKGSVMKLGDNSANMNVETVPTG
                     SLSLDIALGLGGLPKGRIVEVYGPESSGKTTVALHCVAEVQKRGGIAGFIDAEHALDP
                     VYARNIGVDIDNLYISQPDCGEQALEITETMVRSGAVDIVVVDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTAVISKSNCIVIFINQLREKVGVMFGNPETTTGGRALKF
                     YSSVRLDVRRTESLKQGGEIVGNHVRVKVVKNKIAPPFREAEFDIMFGQGISREGDVL
                     DLAVNAGIVNKSGAWYAYEGDKIGQGRENAKTYIHENPAFFDMLEAKVRDFYFTQPED
                     EEDEVQEDSKKPEAEE"
     gene            1831..2439
                     /locus_tag="DHV81_00065"
     CDS             1831..2439
                     /locus_tag="DHV81_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HCI92952.1"
                     /translation="MIEITGYENIGKGKYRTTFDNGTTCVLYRTEAVRYSIMEGAFIS
                     EADYEKLITEVVGKRAKKRALHLLEQMDRSERKLREKLTQGGYPDCCVDAAIDYVKSF
                     HYLDDYRFASTYVRYHQDSLSRRQLEQKLMAKGIGRDDINCAIDSEYTADEEKHIAKL
                     LEKKHYDPDNCDEKEFRRIYQFLMRRGFKSSQILSAMRSQLP"
     gene            2522..>3544
                     /locus_tag="DHV81_00070"
     CDS             2522..>3544
                     /locus_tag="DHV81_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569451.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCI92953.1"
                     /translation="MLPYIITAVVSIIITALIVWFAAALYQKKSANSKIGNAEEKARG
                     IIDEAVKTAEEKKRESMLEIKEESIKAKNDLDKEIKERRNEISRNERRIVQKEENVDK
                     KLEAIEKREAGFAVKEEELKKQKIEISKLNEQRLQELERISGLTSEQAKEYLLKTIEE
                     DVKLDTAKMIKEMENKAKEEANRKAKDYVVTAIQKCAADHVSETTISVVSLPNDEMKG
                     RIIGREGRNIRTLETMTGVDLIIDDTPEAVILSSFDPIRREVARIALEKLIVDGRIHP
                     ARIEEMVEKAQKEVDNMIREEGEAATLEVGIHGIHPELVRLLGKMKFRTSYGQNALKH
                     SIEVAQL"
BASE COUNT         1107 a          567 c          937 g          897 t
ORIGIN      
        1 tttgagtttg agggcatccc gacatttgca cctgagcatt ttgcgagggt atcacttgtt
       61 gattccatga agagaaagca gtttgtaaag ggtgtcacac agatagcgca ggagggtgct
      121 atccagatgt tccaggagta caagggcggc atggaagaga tcatagtcgg cgtggtcggt
      181 gtgcttcagt ttgacgtgct taaattcaga cttgaaaatg agtannnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn aatgtgcaga tcagacttga gaatctgcca tatgagcata
      301 tcagatggat tgagaacaag gatgaggtca atgtagatac aattaacgga acaagcgaca
      361 tgaagaaggt tgtcgatatg aagggcaatc cgcttcttct gttcatcaat gagtggagca
      421 tcggcatgac actcgataga aatgacggac ttgtgcttac tgagtttgga agaaattagg
      481 taaggatttc aaggttgaat ttggacttga cgttagtgag agattatgtg tataatgaag
      541 acagttgtga aggcaactgt ctttttaatt atgttttatt tgtatatttt tgagtatttt
      601 agctatagct gttttagagt atagtgtgag gcacaaacag cagcacgggc agctatttga
      661 ttctgtaatt attccaattt atttgttgac aattgaatag aagtgtatta ttataatagc
      721 agaacgaacg tttgttcgcg agaggatagg agattattat ggcaaaggaa gataaattaa
      781 aagcattaga cgctgccatc gcgcagattg agaaacaata cggaaagggc tctgtcatga
      841 agcttggtga caattcagcc aatatgaatg tagagacagt acctacaggc tcattaagcc
      901 ttgatattgc attaggactc ggaggccttc caaagggcag gattgttgag gtgtacggac
      961 cggagtcatc aggtaagaca acggttgcac tccactgtgt ggcagaggtg cagaagcgtg
     1021 gcggcatcgc cggatttata gatgcagagc atgcgcttga ccctgtatat gccagaaata
     1081 taggtgttga tattgacaat ctctatattt cacagcctga ctgtggtgag caggcacttg
     1141 agataacaga gacaatggta cgttccggcg ccgtggatat cgtggttgtc gattcagttg
     1201 cggcactcgt accaaaggcc gagattgacg gagatatggg ggattcccat gtgggtcttc
     1261 aggcaaggct tatgagccag gcgcttcgta agctcacggc agttatcagc aagtcaaatt
     1321 gtatcgttat attcatcaat cagcttcgtg agaaggtagg tgtgatgttt ggaaatcctg
     1381 agactaccac aggtggaagg gcattaaagt tctattcatc agtgcgtctt gatgtcagaa
     1441 gaactgagtc actcaagcag ggcggtgaga tagtcggcaa ccatgtccgc gtaaaggttg
     1501 ttaagaataa aatagctcct cctttcagag aggcagagtt tgatatcatg tttggacagg
     1561 gtatctcaag agaaggagat gtgcttgatc ttgcagtaaa tgcaggcatt gtcaataaat
     1621 ccggtgcatg gtacgcatac gagggagaca agataggaca gggtcgtgag aatgcaaaga
     1681 cttatatcca tgagaatccg gcattttttg atatgcttga ggcaaaggta agggattttt
     1741 actttacaca gcctgaggat gaggaggatg aagtacagga agactcaaaa aagcctgagg
     1801 cagaagaata ataagtgagc agggttatat atgatagaga ttaccggata tgagaatatt
     1861 ggtaaaggca aataccgcac gacctttgat aatggcacga catgtgtgct gtaccgtaca
     1921 gaggccgtgc ggtactctat tatggaggga gcctttatca gtgaagcgga ttacgaaaag
     1981 ctgataactg aggtggtagg aaagagagcc aaaaagagag cactgcatct gcttgaacag
     2041 atggaccgga gtgaaagaaa gcttagggaa aagctcacac agggcggata tccggattgc
     2101 tgtgtggatg cagcgataga ttatgtcaag agctttcatt atcttgatga ttacagattt
     2161 gcttccacat atgtcaggta tcatcaggac agcttaagcc gcaggcagct tgagcagaag
     2221 cttatggcaa agggcatagg cagagatgat ataaattgtg ccatagacag cgaatacact
     2281 gctgatgaag agaaacatat agcaaagctg cttgaaaaga aacactatga tcctgataac
     2341 tgtgatgaaa aggagtttcg caggatatac cagtttctta tgcgcagggg ctttaagagc
     2401 agccagattc tttctgcaat gaggtcacaa ttaccttgac aatttgcacc aaaaagtata
     2461 gaatttaatt gttgtaattt gtgaaataca atgaaaattt aataaaggag gtgctcctgt
     2521 aatgctaccg tatatcatta ctgctgtagt ttctattatt attactgcat tgattgtatg
     2581 gtttgcagcc gctttatatc agaagaaatc tgctaactcc aagataggaa atgctgagga
     2641 gaaggcaaga ggaatcattg atgaggctgt aaagactgcg gaggaaaaga agcgcgagtc
     2701 tatgcttgag atcaaggaag agtctattaa ggctaagaat gatctggata aggaaatcaa
     2761 agagcgtaga aacgagattt cccgtaatga gcgtcgtatc gttcagaagg aagaaaatgt
     2821 cgacaagaag ctcgaagcta tcgagaagcg tgaagctgga tttgcagtta aggaagagga
     2881 actcaagaag cagaagattg aaatatcaaa gcttaacgag cagcgattac aggaacttga
     2941 aagaatctct ggattaacct ccgagcaagc taaagaatat ctcttaaaga ccattgaaga
     3001 agatgtaaaa cttgatactg caaaaatgat taaggaaatg gaaaacaaag caaaggaaga
     3061 ggcaaaccgt aaagctaagg attacgtggt tacagctatt cagaaatgtg cagcagatca
     3121 tgtgtctgag actactattt cagtagtatc gcttccgaat gatgagatga agggacgtat
     3181 cataggaaga gagggacgta atatccgtac tctcgagact atgacaggtg tagatctgat
     3241 tatagatgac acacctgagg cggttatctt gtcaagcttt gatccaatta gaagagaggt
     3301 tgcaagaata gctcttgaga agcttattgt tgacggaaga atccatccgg cccgtataga
     3361 ggagatggtt gagaaggctc aaaaagaagt cgataatatg attcgtgaag agggtgaggc
     3421 tgccacactt gaggtgggca tacatggtat tcacccggag cttgtgcgtc ttcttggaaa
     3481 gatgaagttt agaacaagct acggtcagaa tgcgttaaag cattctatcg aggttgcaca
     3541 gctg
//
LOCUS       DPUF01000004            9536 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6563, whole
            genome shotgun sequence.
ACCESSION   DPUF01000004 DPUF01000000
VERSION     DPUF01000004.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6563"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="DHV81_00075"
     CDS             complement(<1..215)
                     /locus_tag="DHV81_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCI92954.1"
                     /translation="MRNKKVLFAVQAAMIAALYVVLTYITNLLGLASGTIQVRFSEAL
                     CILPVFTPAAIPGLFIGCLISNLITGG"
     gene            401..1447
                     /locus_tag="DHV81_00080"
                     /pseudo
     CDS             401..1447
                     /locus_tag="DHV81_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516705.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
     assembly_gap    846..907
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1527..2306
                     /locus_tag="DHV81_00085"
     CDS             1527..2306
                     /locus_tag="DHV81_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HCI92955.1"
                     /translation="MSVYKVDICGVTTSKLPVLKDDEKEELFEKIKKGDKAAREKYIR
                     GNLRLVLSVIRRFSNHNENMDDLFQIGCIGLMKSIDNFDPTLGVKFSTYAVPMIIGEV
                     RRYLRDNSAYRIPRSLRDTAYQAIKSKEKLSRKLVHDPSLAEISKDCGIPENDILYAL
                     DAIQTPLSLFDPVYTDGGDTLYVMDQISDKKNKEEAWVKNLVLDDALKLLDDRARQII
                     KLRFFEGKTQIEVAEEINISQAQVSRLEKNALKNMHGYLEA"
     gene            complement(2323..2863)
                     /locus_tag="DHV81_00090"
                     /pseudo
     CDS             complement(2323..2863)
                     /locus_tag="DHV81_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516703.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
     assembly_gap    2434..2471
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3054..4847
                     /locus_tag="DHV81_00095"
     CDS             3054..4847
                     /locus_tag="DHV81_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCI92956.1"
                     /translation="MKKLINLISEEVTKAFVSAGYDEKYGKVTLSNRPDLCEFQCNGA
                     MAAAKEYKCAPFMISDKVAALLESDEMFESVESVKPGFLNIKMDTAFLAKYMNDMKDD
                     EGRYGLEKAKKPLTIVVDYGGPNVAKPLHVGHLRSAVIGESVKRIAKFMGHNVIGDVH
                     LGDWGLQMGLIITELRERKPDLVYFDESYTGEYPKEAPFTISELEDIYPTASGKSKSD
                     ESFKEAALLATKELQGGRRGYQALLSHIMNVSVTDLKRNYENLNVHFELWKGESDAQP
                     YVPGMVEMMKEKGFAHMSEGALVVDVKEDTDTKEIPPCIILKSDGASLYSTTDLATLV
                     MRMKENNPDRVIYLADARQSMHFIQVFRCARKTGIVPDTTELVHIGFGTMNGKDGKPF
                     KTRDGGVMRLEYLLKEIDDEMLKKIMENQKEKENLNIDEAEAEQTAKTVALAAVKYGD
                     LSNQASKDYIFDIDRFTSFEGNTGPYILYTIVRIKSILSKYEAKGGDISALKDAIVPA
                     VNAGQKNLMLSLAKFNATIESAYEESAPHKICAYIYELANAFNGFYHDTKILSEENEE
                     LKKSYISLLVLTKEILEACIDMLGFSAPDRM"
     gene            5251..6150
                     /locus_tag="DHV81_00100"
     CDS             5251..6150
                     /locus_tag="DHV81_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HCI92957.1"
                     /translation="MDIIDYLPQKMRTEAEAYMPCGLEEIRVRAGKPVQYRFAGGNTT
                     GVNILQSDIEEMINYLSGYSIHAIAPQLAAGYFTVEGGHRVGIAGQMGCDGERVTAVS
                     EIASLNIRIAHQIKGIAMPLMPYIRDENSIYNTLVVSRPGEGKTTFLRDCIRILSDGC
                     DGKNALKVSVVDERSEIAACYNGVAQNDVGKSSDVLDNCPKSLGMRMLLRSMSPDVIA
                     VDELGGESDIEALNQLVSCGIRILGSVHGTNADDLRRKMLPRAIERFVFIDHDKKGVR
                     CYSIYDEAHRLLYSTVCGIGADK"
     gene            6182..6607
                     /locus_tag="DHV81_00105"
     CDS             6182..6607
                     /locus_tag="DHV81_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI92958.1"
                     /translation="MLYDFMLLLEKGAFYLAGERMKAPQFFSSIRGKSEVFNEGCKRM
                     GEALSNRSCKSGEEGWRSVWEDVLCGRISDEKIKKMIVEMGGAFFEKNAAAMEQRLKE
                     CALDIHKQIDFYRKEYVEKMKVICPVGILGGIMISIMLI"
     gene            6675..6869
                     /gene="spoIIIAC"
                     /locus_tag="DHV81_00110"
     CDS             6675..6869
                     /gene="spoIIIAC"
                     /locus_tag="DHV81_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HCI92959.1"
                     /translation="MEISIIFKIAALGIVITVLGQVLKHSGREEMTFLLSLAGLVIVL
                     FWIVPYISELFKTMQDMFSL"
     gene            6871..7248
                     /locus_tag="DHV81_00115"
     CDS             6871..7248
                     /locus_tag="DHV81_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HCI92960.1"
                     /translation="MLKIGIIGIAAVFFAVLLKRDRPELSMLLSVACGTIIFAFALER
                     LGTTIEFFKGLLSKLPVEPAFFVTMVKMLGIAYIAEFSSSICKDCGHSAIASQIELFA
                     KVAIVVMSIPVMSCLIELIGELF"
     gene            7245..>8224
                     /locus_tag="DHV81_00120"
     CDS             7245..>8224
                     /locus_tag="DHV81_00120"
                     /inference="COORDINATES: protein motif:HMM:PF09546.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI92961.1"
                     /translation="MICRKNIFIIIMLIVMFLPQDCVYASDTEITDTIEQQMLEKIDM
                     SDVEKYSKDYLPDRLSFSDIVDALLDADQKAGEKICEYIYELFFYEIAAVKPVIINVL
                     CYTILFMVFSRLIFWRQDYVYNVSFLFMYASVISMLMASFFVLSDVAKGCIDVLLTYL
                     TALVPAYATVLLASGKGFSASGFYVLAFVLIYVVEWLIKLVLIPGIHLFLVLEVLNHV
                     SSEKKLEKLAEFIESAVTKIMKASIKIVAGIGIVQAMIAPAKDKISESLILKSFQAIP
                     GVGRLGQGAGEIMLGCAMLIKNSVGMAAIIVLFFIVLTPLVKTLIFSLMY"
     assembly_gap    8225..8293
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8423..8674
                     /locus_tag="DHV81_00125"
     CDS             8423..8674
                     /locus_tag="DHV81_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HCI92962.1"
                     /translation="MKSYLYLALFFMVMTYLLPREQYRRYFRFMLGVFLAVIILKPLA
                     AGYEYDLRFAELTKKIEGISYEYDADATDLFKAFEAEEN"
     gene            8655..>8938
                     /locus_tag="DHV81_00130"
     CDS             8655..>8938
                     /locus_tag="DHV81_00130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HCI92963.1"
                     /translation="MKLKKTDYIVILLVGVLLLIVVLPVKEEKKTCSDSQKKAKAKSV
                     SESTYDDEEYEKLLEKKLESILERMDGVGEVSVMITLSDDGTRIVDKDTK"
     assembly_gap    8939..9120
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9222..>9536
                     /locus_tag="DHV81_00135"
     CDS             9222..>9536
                     /locus_tag="DHV81_00135"
                     /inference="COORDINATES: protein motif:HMM:PF12685.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AH"
                     /protein_id="HCI92964.1"
                     /translation="MKKGSHKNQIIITTLALLLAVVGYISYDNRDTFMNAKDVLSTEI
                     EAVNGEADTQVSDEVALETDSTEEILNAGETVLTSASTESEECVAQVKLGREQVRSKN
                     KEA"
BASE COUNT         2826 a         1508 c         2357 g         2494 t
ORIGIN      
        1 atgccgcctg tgataagatt ggaaatcaga catccgatga aaagacccgg aatggctgcc
       61 ggtgtgaaaa ccggaaggat gcaaagcgct tcagaaaagc gtacctgaat ggtgcctgat
      121 gcgagaccaa gcagattggt gatatacgtg agcacaacat agagtgctgc aatcatagct
      181 gcctgtactg caaataatac ttttttgttt ctcatattta gttctccttt agttttgttt
      241 tacgtcagga aggtttcgaa ctgacgcaat tatttcgcct tgctatgtta acaaaaaatg
      301 ctttgaatgt caagaagttc ataaagtcag tgcccattta gagaagttgt ggtataatga
      361 gtgcaagtga gaagataaat gacaagaggg aaacagtatt atgagagaaa taaaggttaa
      421 tgaactaaaa gagggaatga ctacggcagt agatgtgttt tcgccaaagg gacagcttat
      481 ccttaaaaga catcaggctg tatcggcatt tgatatagca aagtttggat tctacaatat
      541 agcttcggtt tatgttgagg gaagctcagc acaggaaaaa gaagaatgga ataaaaaata
      601 tgccatcatt aaggaaaaat accgtgattc catcgataac ctgcatgaat atatgaatga
      661 catactatac aggaatatca tcccggataa gaatacgctt atcagggact cggtagagat
      721 ttttgacagg tttgagactt catatgagct atttgacgca cttcaggtat taaagcagac
      781 agatgtatct acgatggcac attccatgaa tgtatcaatt attgcacgtc tcattggtgt
      841 atgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnata ggcaagttta agataccgga tgagatatta ttgaagccgg gaaagctcac
      961 aaaggaagag tttgaggtga taaagaagca cacggtatat ggctatgaga tacttaataa
     1021 tttcaagatt cttgaaagca cgaagcgtgc ggcactttta catcatgaga agtttgacgg
     1081 ctcaggatat ccgtttggat atacagcaga taaaatcgat gatatatcag ctattattac
     1141 gatagccgat gtctacgatg caatgacgtc aaagcgctgt taccgtgatg ggatgtgtcc
     1201 gtttgatgtg atagcagatt ttgagtatga tggcatatca aagtatcatc cgaagtatat
     1261 agggatgttt ttgaagaaga ttgcaagctc atatatcgga agcactgtgc ttttgacaaa
     1321 cggcaaaaag gccaaaataa tttttgtgac agagaagtat tcaaggccta cagttacatt
     1381 gcttgaagac aacagtgtgc tggtgcttaa ggatgaaaag gatataaaaa tagcggcagt
     1441 gctgtagaat atatacgcat attaattgaa taaaattact gtaaatggga atgctttaga
     1501 taaagaaatt tatccggagg attcccatgt ctgtatataa ggttgatata tgtggagtga
     1561 ccacatcaaa actgccggta cttaaggatg atgaaaaaga agaactgttt gagaaaataa
     1621 aaaagggaga taaggctgca agagaaaagt atattcgtgg aaatttaagg ctggtgcttt
     1681 cggtaatcag aaggttttca aaccataatg aaaatatgga tgatttattt cagataggat
     1741 gcatcggact tatgaaatca attgataatt ttgatccgac tttaggtgtt aagttttcca
     1801 cctatgctgt tccgatgatt atcggtgagg tgagacggta tctgcgtgac aacagtgcat
     1861 accgcattcc acgttccctc agggatacag cttatcaggc tattaaatca aaggaaaagc
     1921 tatcgagaaa gctggtgcat gatccgtctt tagcggaaat atcaaaggat tgcggcatcc
     1981 ctgaaaacga tatactatac gcacttgatg caatccagac accgctttcc ctctttgatc
     2041 ctgtatatac tgacggtggt gatacacttt atgtgatgga tcagatatcg gacaagaaaa
     2101 acaaagagga agcatgggta aaaaatctgg ttctcgacga tgcattaaag ctgcttgatg
     2161 acagagcgag acagataata aagctgcgat ttttcgaggg caagacacag atagaggtgg
     2221 cagaggagat aaatatctct caggcacagg tatcaaggct tgagaaaaat gcgcttaaaa
     2281 atatgcacgg atatctggaa gcataagcag ataattgaat atttagtctg cctgtgctaa
     2341 agaagttttt ttaagcacag ccgcaattat gactgcatat acacctccga taacagcggt
     2401 gataagctgt gtcactgaaa actgaagctg taannnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nggtagaatg ataagtgcaa tggctattcc catgaaaacg gcctttagta
     2521 tggcacctat tgcaattgat acaggaaaac cggcagcttt gccgcatttt ttgcgaagca
     2581 gtcccacaca tgccacgaga atgatatttc ctatcatgat acatggaaac atcgcaggga
     2641 tagccgccat aacaggactt cctgtgatga aaaatgatgt gacaggtgtg ataatgctta
     2701 ggataatgcc acatgccatt ccggcataga gtacggtaag tattaaagct gcattaatga
     2761 ttggacctgt gatgtatacg cttaagttct taaagaactg gctgacaagg cagattgcca
     2821 gcataattgc agtaacggtt atttgcttag tttttatctt cattgataag acctccttta
     2881 aattttcata tgaaatcata ctcctttggg ggtgcaaatg caaggttacg ttgacggtta
     2941 atatttactc agaaggcaaa aatgacggtt gatattgaaa gcaattcatt tgtgtgctaa
     3001 tataagtata ttcgctgttt gacgcaaaac agctatatat caggaggaat acaatgaaaa
     3061 aactgattaa tttgatatca gaggaagtga caaaggcatt cgtcagtgca ggatatgacg
     3121 agaagtacgg aaaggttaca ctttcaaaca gaccggacct ttgtgagttc cagtgtaacg
     3181 gagcaatggc agcggcaaaa gagtataagt gtgcaccatt tatgatctcg gacaaggtag
     3241 cggcgctcct tgagtcagat gagatgtttg agtcagtaga atcagtaaag ccgggatttc
     3301 ttaatatcaa aatggatacc gcttttctgg caaaatatat gaacgatatg aaggatgacg
     3361 agggcagata cggcttggag aaggccaaaa agccactcac tatagtagtg gattacggcg
     3421 gaccaaacgt cgcaaagcct cttcatgtgg gacaccttcg ctcagcagta atcggagaga
     3481 gcgttaagcg tatcgcaaag ttcatgggac acaatgtgat tggtgatgtg catctcggtg
     3541 actggggact tcagatggga cttatcatca cagagcttcg cgagagaaag cctgacctcg
     3601 tttattttga tgagagctat acaggagagt acccgaagga ggcaccgttt accatatcag
     3661 agcttgagga catctatccg acagccagcg gcaagtcaaa atcagatgag agcttcaagg
     3721 aagcagcact tcttgccaca aaggagcttc agggaggcag gagaggttat caggcactgc
     3781 tttcacacat catgaatgtc tctgtaacag atcttaagag aaattatgag aatctgaatg
     3841 ttcattttga gctgtggaag ggcgagtcgg atgcacagcc atatgtacct gggatggttg
     3901 aaatgatgaa ggaaaagggc tttgcacata tgagtgaggg cgctttggtt gtggatgtga
     3961 aggaggatac agatacaaag gagattcctc catgtatcat cctaaagtct gacggagcct
     4021 cactttacag cactacagat ctcgcaacac ttgttatgcg tatgaaggag aacaatcctg
     4081 accgtgtgat ttatctggca gacgcacgtc agtcaatgca ctttattcag gtattccgct
     4141 gcgcaagaaa gacaggcata gtacctgata ctacagagct tgtacatatc ggttttggaa
     4201 ccatgaacgg caaggacggc aagcctttca agacaagaga cggtggagta atgcgtcttg
     4261 agtatctcct taaggaaatt gatgatgaga tgctcaaaaa aatcatggag aatcagaagg
     4321 aaaaagagaa tttaaacata gatgaggcag aggctgaaca gactgcaaag acagtagcgc
     4381 ttgcagcagt caagtatggt gatttgtcta atcaggcgag caaggattac atctttgata
     4441 ttgacagatt cacatccttc gagggcaaca caggtccata tatcctttat acaatcgtgc
     4501 gtatcaagtc aatcctctca aagtatgagg caaagggtgg cgacatcagc gcattgaagg
     4561 atgcaatcgt gccggcagtc aatgcaggac agaagaatct catgctctct ctggcaaagt
     4621 tcaatgctac aattgagagt gcatatgagg agtctgcacc acacaagata tgtgcataca
     4681 tctacgagct tgccaacgca ttcaacggct tctaccatga cacaaagatt ctttctgagg
     4741 agaatgaaga gcttaagaag tcatatattt cgctgcttgt gctcacaaag gagatacttg
     4801 aggcttgtat cgatatgctt ggattctcag ctcctgacag gatgtaaaaa ataataattt
     4861 tcacgttcta aaacaattgg acatgtaata taaataggac atagatgatt tatcagaatc
     4921 gtctgtgtcc tttttttaat ttgaataact gtcggtggca ggtcgggatg ggctcacgcg
     4981 tgtggctgca gtgtcgcaga agcgtatagc ggagctattt agagtctgtt aaattatgct
     5041 ttgaagcaca gaaatgccga ggcatggacg ccgaggcaag gagatatcga gcccggacgg
     5101 cgagtatcga cggtttcgtc aggcatagaa gtacttattg cataatttat acagagtctt
     5161 gatagcgcag cgtagcatct gcgacactgc agccacacgc gtgcgccata tcccgacctg
     5221 ccaccgaagt ggcgataagg agtgaaaaat atggatataa ttgattatct gccacaaaag
     5281 atgaggactg aggcagaagc gtacatgccg tgtggtctgg aggaaatcag ggtgcgcgcg
     5341 ggaaagccgg tgcagtatag gtttgcggga ggcaatacaa caggagttaa tatcttacaa
     5401 agcgatatag aggagatgat aaattatctg agcggatatt caatccacgc gatagcaccg
     5461 cagcttgccg caggctattt tacagtggag ggaggtcatc gtgtgggaat tgcagggcag
     5521 atgggctgcg atggagaaag ggttacagca gtcagcgaga tagcatcctt aaatatcaga
     5581 attgcgcatc agataaaggg tattgcaatg ccactgatgc cttatataag agatgaaaac
     5641 agtatttata atacattggt ggtatcacgg ccgggagaag ggaaaacaac atttttaaga
     5701 gactgtatca ggatactttc agatggctgt gacggaaaaa atgctcttaa ggtgtcggtg
     5761 gtggatgagc gctcggaaat agcagcgtgc tacaacggag tggctcaaaa cgatgttgga
     5821 aaatccagtg atgtgctgga taattgtccg aagtcacttg gcatgaggat gcttttgcgt
     5881 tctatgtccc cggatgtgat tgctgtggat gagctggggg gcgaaagtga tatcgaggca
     5941 ctaaaccagc ttgtgagttg cggaataaga atacttggca gcgtacatgg caccaatgca
     6001 gatgatctgc gaagaaaaat gctgccacgt gcaatagaga ggtttgtgtt tatagaccat
     6061 gataaaaaag gggtgagatg ctatagcatt tatgatgaag ctcacaggct tttgtatagt
     6121 actgtttgcg gtattggggc tgataaatga ttacagcaaa agaaaaaggg aggatatagc
     6181 aatgctgtat gatttcatgc ttttacttga aaaaggggca ttttatctgg caggagaaag
     6241 aatgaaggca ccgcagtttt ttagcagcat aagaggaaaa agcgaggtgt ttaatgaggg
     6301 atgcaagaga atgggagagg cactttcaaa caggagctgt aaaagtggag aggagggctg
     6361 gaggtctgtg tgggaggatg tgctttgtgg caggatatct gatgaaaaaa taaagaaaat
     6421 gatagttgaa atgggaggtg cattctttga aaaaaatgcg gccgcgatgg agcagcggct
     6481 taaggaatgt gccttggaca tacataaaca gatagatttt tacaggaaag agtatgttga
     6541 aaaaatgaag gttatttgtc cggtgggtat tttgggaggg attatgatat caataatgct
     6601 tatataaaaa tgtttgtata aaaatgtggt aatccgatta aacatattgt tgtatgcgat
     6661 gaaaggactt gatgatggag atttcgatta tttttaagat agcggcactt ggaatagtga
     6721 ttacggtgct cggacaggtc ttaaaacaca gcggaaggga ggaaatgact ttcctgctta
     6781 gtctggccgg acttgtgatc gtattgttct ggattgtgcc atatataagt gagctgttta
     6841 agacaatgca ggatatgttt tctttgtaat atgttgaaaa ttgggataat aggaatagcg
     6901 gcggtgtttt ttgcggtgct tttaaagcgt gacagacctg agctgtcaat gcttctttca
     6961 gtggcctgcg gtactataat ttttgctttt gcattggagc ggctcggcac aacaattgag
     7021 ttttttaaag gacttctctc aaaactgcct gtggaaccgg ctttttttgt cactatggta
     7081 aagatgctcg gaattgcata cattgcggag ttttcatcct caatctgtaa ggactgcggt
     7141 catagcgcga ttgcatcgca gatagagctt tttgcgaagg tggcaattgt tgtgatgtca
     7201 attcccgtga tgtcgtgtct gatagagctg attggagaac tgttttgatt tgtaggaaaa
     7261 atatttttat aataatcatg ttgattgtga tgtttttgcc gcaggactgt gtgtatgcat
     7321 cggacactga aattacagat acaattgagc agcagatgct tgaaaaaata gatatgtcag
     7381 atgtggaaaa atattcaaag gattatctgc cggacagact atcgttttcg gatattgtgg
     7441 atgctctttt agacgcagat caaaaggcag gcgaaaaaat ctgtgagtat atttacgagc
     7501 tgttctttta cgagatagcg gcagtgaagc cggttattat aaatgtgctg tgctacacga
     7561 tactttttat ggtgttttca aggcttattt tctggcggca ggattatgta tacaatgtaa
     7621 gctttctgtt tatgtatgca tcggttatat caatgcttat ggcatctttt tttgtgttgt
     7681 ctgatgtggc aaagggctgt attgatgtgc ttttgactta tcttacagcg cttgttccgg
     7741 catatgcgac agtgcttctt gcgtcaggaa aaggattttc tgcatcaggc ttttacgtgc
     7801 ttgcatttgt actcatttat gtagtagagt ggcttataaa gctggtgctc ataccgggaa
     7861 ttcatttgtt tctggtgctt gaggtgctca atcacgtatc tagtgaaaag aagcttgaaa
     7921 agcttgcaga gtttattgaa tcagcagtta caaaaataat gaaggcatcc ataaagatag
     7981 tagcgggcat aggcatagtc caggccatga ttgctcccgc aaaggacaaa atatcggaaa
     8041 gccttatctt aaaaagcttt caggcgatac cgggtgtggg gcggcttggg cagggggcag
     8101 gtgaaattat gcttggctgt gcgatgctca ttaaaaacag tgttggtatg gcggcgatta
     8161 tagtgctttt tttcattgtg ctgaccccgc ttgtgaaaac actaatcttt tcgctgatgt
     8221 acagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnngagggca tagatgccgt tgcgagggca ggaaatctgt attacattgt
     8341 gataagagat gcatctatat tattttttat cgtgatagcg atagtgtgtg catccacaag
     8401 ctttatggga ggttgacaag gtatgaagag ctatctgtat ctggcacttt ttttcatggt
     8461 aatgacatat cttttgccga gagagcagta caggaggtat tttaggttta tgcttggggt
     8521 atttttggct gttattattc taaagcctct tgcggcaggg tatgaatatg acttgagatt
     8581 tgctgagctt acaaagaaaa tagaggggat ttcgtatgaa tatgacgcgg atgcgacaga
     8641 tttgtttaag gcttttgaag ctgaagaaaa ctgactacat agtgatttta cttgttggag
     8701 tgctgcttct tattgtagtg cttccggtaa aagaggaaaa aaagacctgt agtgattcgc
     8761 aaaagaaggc taaagccaag tcggtaagtg aaagcaccta tgatgatgaa gaatatgaga
     8821 agctgcttga aaaaaagctt gagagtattc ttgagaggat ggacggtgtg ggcgaggtgt
     8881 ctgtgatgat tacgctgtca gacgacggca caaggatagt ggataaggat accaaggann
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 ccacagataa acaatgatat ttctaatgcg gtttctgcat tatttgatgt gcccatgcat
     9181 aaaataaaaa tagcaaaaat gatatccagg gaggaataaa agtgaagaaa ggcagccata
     9241 aaaatcagat aattattacg acgcttgcac ttcttttggc agtggtcgga tacataagct
     9301 acgataacag ggataccttt atgaatgcaa aggatgtgct ttccaccgaa atcgaagctg
     9361 taaatggcga agcggataca caggtatcgg atgaggtggc acttgagaca gactcgacag
     9421 aggagatatt aaatgccggt gaaacggtac tgacaagtgc atccacagag agtgaggagt
     9481 gtgtggcaca ggtgaagctg ggacgtgagc aggtgcgttc aaagaacaag gaggct
//
LOCUS       DPUF01000005            3192 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23473, whole
            genome shotgun sequence.
ACCESSION   DPUF01000005 DPUF01000000
VERSION     DPUF01000005.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23473"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..>3192
                     /locus_tag="DHV81_00140"
     CDS             <1..>3192
                     /locus_tag="DHV81_00140"
                     /inference="COORDINATES: protein motif:HMM:PF05737.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding protein"
                     /protein_id="HCI92965.1"
                     /translation="VEFTVDVSSGKGTPDDIEITDEFNGNGMRVDTTGFTYSIKKNGQ
                     AVDSSYTATATGKNDPADAGKATLTAKLPKLSAGESCSITYRYYLTNAGDLGNNAAWQ
                     ATNFVTGKSTDQKSKETVIDTSSSYVKYYKNLIEKSGKYDKTNNRIIWTVTVNKSCED
                     IAGAVLTDDMFDKITGIDISPSDGYTINKDSSGKVTSVTFNPTDGNMNCNKYTVKYYT
                     DALRTFDDQKFNNKATLTKDDKSYDGSSSVNVPKTKQDITKKLVEAQKTDNGLYTLKW
                     QAKFTIPASGFPKEFGIADTMSSKSQKNCHYMTYTQMNDFVSQVKKEFDGYIGDITVK
                     YLDENNNSQKADFSKLSETADYRFTSVNISFNKDYPISNSERTVAFTYSTTADTTVSG
                     RTEYVNKFEALNSSASASYSHVTSEIKKTDGNGREGQTYLKTTESDGTLTWRVDVLMD
                     ADATEYAITDKLPKGVTLQSVRIMLAYPSNDGYTYSYFDGTWDTVSVGDWFTDGIKTS
                     TMLDESTNTVVTRVVNPDKNNTTIHGWFENSHIYVVYKCTIDDFENFEAGKSVDYKNI
                     ATASSNTNSDMGWVKQTQTMSKPGESGSDNPTEGDKVVSKNFKWDEDNHRLRYTVIIN
                     PDGKDYLPDSDTLKLTDVLQFQTMTFSPQALWYIDLMPATVQFRQAIKQDDGTYKAGD
                     TVDGCKWAYTTKDGTYEWNDTSYRTISADIPDGKAIMLTYTYQVNAKVLGTPDQYNPI
                     KMDVSNTARLEGVEKGEDRKDNQTVYKESKSSAGVIKSNTFTLYKVDKTNYGKQLEGA
                     EFELFGYTSDGSYKSLGKYTTDKNGRIDISMSTDNLEYNTQYYVVETKAPDGYILSTE
                     PHKEYFYFSADTSAHPVVAENSSLQGSDMAKVNTPYYYEDTAVSTTSISVDKKWTDSK
                     NNPLSKTDGKIYLQLHQVDSAGNDKKYGNPVEVTADSAGEWSYVFENLPLQSVDENGI
                     LTGTTYRYYVTEVGINQNNSMSGYDVSYIFKDINGTQITKTDANVAPGSANAIESGTV
                     EITNKLIEYQLPETGGSGNRWLYMLSGVVLIAIA"
BASE COUNT         1106 a          528 c          741 g          817 t
ORIGIN      
        1 gttgagttta ctgtggatgt gtcttccgga aaaggtacac ctgatgatat cgaaatcaca
       61 gatgaattta acggcaatgg tatgagggtt gatactaccg gatttaccta cagtataaag
      121 aaaaacggtc aggcagttga cagtagttat actgcaacag cgacaggaaa gaatgacccg
      181 gcagatgcgg gaaaggcaac acttactgca aagcttccaa aattaagtgc aggtgagagc
      241 tgttctatta catacaggta ttacctgacc aatgccggag atttgggaaa taatgctgca
      301 tggcaggcaa ctaatttcgt taccggtaaa tcaacagacc aaaaatcaaa agaaactgtc
      361 atagatacat caagcagcta tgtgaagtat tacaaaaatc ttatagaaaa atccggtaaa
      421 tatgataaga ctaataaccg gattatctgg acggttacag taaataaaag ctgtgaggat
      481 attgcgggag ctgtcctgac agatgatatg tttgataaaa ttacaggcat tgacatatca
      541 cctagtgatg gatatacaat taacaaggac agcagtggca aggtgacatc ggttacattt
      601 aatcctacag atggaaatat gaactgtaat aagtatactg tcaaatatta tacagacgca
      661 ttgcggacat ttgatgacca gaaatttaat aataaggcta cccttactaa ggatgataag
      721 tcatatgatg gctcttcaag tgtaaatgtt cctaaaacaa agcaggatat tacgaagaag
      781 cttgttgaag ctcaaaagac agataacggg ctgtatactt taaagtggca agcaaaattt
      841 acaattccgg catcgggatt tcctaaagag tttggcatag ccgatacaat gtcatccaaa
      901 tcccagaaga attgtcatta catgacttat acccagatga atgattttgt aagtcaggta
      961 aagaaagagt ttgatggata tatcggtgat attactgtta aatatctgga tgagaacaat
     1021 aattcgcaaa aggcagattt tagtaaatta tcagaaaccg ctgactacag atttacctcg
     1081 gtaaatatca gctttaataa ggattatccg ataagcaaca gtgaaaggac agtcgcattt
     1141 acatacagca caaccgccga cacgacagtg agtggaagaa ctgagtatgt gaataagttt
     1201 gaagctttaa attcaagtgc atctgcatct tacagccatg tgacatcaga gattaaaaag
     1261 actgatggaa acggccggga aggtcagaca tatttgaaga ctacggaaag cgatggaaca
     1321 ctcacatggc gcgttgatgt acttatggat gccgatgcaa cagagtatgc tatcactgat
     1381 aagcttccaa aaggtgttac actacagagt gtaaggataa tgcttgcata tccgagtaac
     1441 gatggctata catattctta ctttgacggt acatgggata ccgtatctgt cggtgattgg
     1501 ttcacagacg gaattaaaac aagtacaatg ttagatgagt ccactaatac cgtggtaaca
     1561 agggttgtaa atcctgataa gaacaataca acaatacatg gttggtttga gaactctcac
     1621 atatatgtag tatacaagtg tacgatagat gattttgaaa attttgaggc aggtaaatca
     1681 gtggattata agaatatagc cacagcatcc tcaaatacaa acagtgatat gggatgggtt
     1741 aaacagacac agaccatgtc aaagcctggc gaatcaggca gtgataatcc tactgagggc
     1801 gataaggttg tttccaagaa cttcaagtgg gatgaggata atcacaggct gcgttataca
     1861 gttataataa accctgatgg caaggactat ctgcctgata gtgatacatt aaagctaaca
     1921 gatgtgcttc aattccagac tatgacattc agcccacagg cattgtggta tatagatcta
     1981 atgccggcta ctgttcagtt caggcaggca ataaagcagg atgatggcac atacaaagca
     2041 ggcgatacgg tagatggctg taagtgggcg tatacaacta aagatggcac gtatgaatgg
     2101 aatgatacat catacaggac aatcagtgca gatatacctg atggcaaggc gataatgctt
     2161 acttatacat atcaggtgaa tgcaaaggtg ctaggtacgc cggatcaata caatcctata
     2221 aagatggatg tcagcaatac agcaaggctt gagggtgtcg aaaaaggaga agacagaaag
     2281 gataaccaga ctgtttacaa agagtcaaaa tcatctgccg gagtaattaa gagtaataca
     2341 tttaccttgt ataaggtgga taagactaat tatggaaagc agcttgaggg cgcagagttt
     2401 gagctgtttg gatatacatc ggatggttca tacaagtcgt taggcaagta taccactgat
     2461 aagaacggca gaattgatat cagtatgagt actgataatc tggagtataa tacgcagtat
     2521 tatgtagttg agacaaaggc tccggatggt tatatactct cgactgagcc gcataaggag
     2581 tatttctatt tcagtgcaga tacttcggca caccctgtcg tggctgaaaa cagctcatta
     2641 cagggaagcg atatggcaaa ggtaaataca ccgtactatt atgaggatac agctgtttcg
     2701 acaacaagca tttcggttga taaaaagtgg actgattcaa agaataatcc attaagtaag
     2761 actgatggaa agatttatct gcagctgcat caggtggatt cggctggtaa tgataagaag
     2821 tatggcaatc cggttgaggt gacagcagat tcggcaggtg aatggtcata tgtatttgaa
     2881 aatctgccat tgcagagtgt agatgaaaat ggtattctga ccggtaccac atatagatat
     2941 tatgtgacag aggttggaat aaatcagaac aacagtatgt caggctatga tgtttcatat
     3001 atatttaagg atataaatgg tactcaaatc acaaagactg atgcaaacgt tgcaccgggc
     3061 agcgccaatg caatagagtc cggtactgtt gagattacaa acaaactcat agagtatcag
     3121 cttccggaaa caggtggaag cggtaacaga tggttgtata tgttaagtgg agttgtgctt
     3181 atagcaatag ct
//
LOCUS       DPUF01000006           11382 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1439, whole
            genome shotgun sequence.
ACCESSION   DPUF01000006 DPUF01000000
VERSION     DPUF01000006.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1439"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="DHV81_00145"
     CDS             complement(<1..905)
                     /locus_tag="DHV81_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCI92966.1"
                     /translation="MAGPKAPKDPERKRPYFYIMKDKDIYGSVQEDGSIIHFIYESDG
                     RLINSAQIAGNIENKEELGLLETVEGFGRLVHSIGVSVETDNQNEQIEFVFQMYGKQD
                     LYGGGTNLKVKLTGDGMERKIYLSDYKWTPDDDIPGQIKFIFNTPDIMGKASVRLYLN
                     DGYEAPADIEETEVDMNSDEYCSMISHSLMNMGNVYRIRKAIEKTRAGKEVTLAYIGG
                     SITQGAGATPINTECYAYKSYQLFQRRFSAKNNVKFIKAGVGGTPSELGMIRFDRDVL
                     RDGQQPDIVVIEFAVNDEGDETKGD"
     gene            complement(1019..4051)
                     /locus_tag="DHV81_00150"
     CDS             complement(1019..4051)
                     /locus_tag="DHV81_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI92967.1"
                     /translation="MLATGVTQLIPAYQAQSLVFADEVQQNIKKTTYTADKLTVDWGN
                     AGYELTDGVWKVTFNNQYDQVKWRLPETVDMSTVKSVTFNVKDQKGSVSLKVYKNGDE
                     DADGANTKYELTGADEYSISPTGEGDMVAVGLMTTDNAGSGSAISLVSVTVETDTTPK
                     TPPEIEQNIEDWKKSVTSSDALGENAIAGTGIMLDEIKDNTLMDLVEKHFNAVTFGNE
                     LKPDALFNYQLEKSVKTKTVQFDGQDLEVPMVNDAGDSLDFSRADAMVDKILEWNAAH
                     PDRKIRIRGHVLVWHSQTPEWFFHENYDISKPYVDKETMNRRLEWYISSVFDHYFGEA
                     ANKKYDGLFYGWDVVNEAVIGNTYRTDTVSPAESLDEIRHGNNSSWWHVYKSNEFIIN
                     AFKYANKYAPSDVELYYNDFGETDNIKSEGIIKLISDVKSAQGTRLDAFGMQAHYSVD
                     SFSAAQFKTVAKKYAEAAGKVQLTELDFQASAAYKSGAASKESEYTKMAYCHKQLFDA
                     AKDLKKNGTNVAGITVWGVIEPNSWLHSQSNVGGGADGSKQCPLLFDGKYKAKPAYWA
                     YVDATKLEPLIQDIVVAEQKGDTMSGTEYSFSDDDTQAAFIPTWDKDGLNVLVSVKDA
                     TINDTDEVTVYVDETNSAGDVTPVKKTVKRSEAQAVDGGYRATIKVPMTDLKVAKTIG
                     MDVKVMNNDKAVSFNDLKEMQETSSKYYAKATLKPGIEKATKATVKIDGEADSEWDKA
                     VAIPLTINLGAKVTADAKVLWDDENLYVYATVKDPVLNKDGGEAYQQDSLEVFIDENN
                     AKTESYDDDDKQYRINYENEHSFNGKKCLEENVQSAAKVTGDGYVIEAAFKWTDIKPK
                     KGDRIGLEFQINDADASGARIGTLSWNDETGMGWSKSSVYGTIELAAEAKDEVSDDNS
                     KPGTDDPSTGNTEKPGTNNSSTGNTEKPGTGKPAINKPSADKPATGSTNKPLANKPAA
                     KKAAKTSDQSATAAFIVLFLAGVSMAGASRLRRSRKQS"
     gene            complement(4273..>4661)
                     /locus_tag="DHV81_00155"
     CDS             complement(4273..>4661)
                     /locus_tag="DHV81_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HCI92968.1"
                     /translation="EIFGRHDWYYSDAYRTIANIAMDHLWFDADPWQIKTAENLQKFY
                     CEDQKDNWDGVFLIDGTPLDEKALHPVAIIAVNAEASLAADGKYAKQCVEKFWNTPLR
                     TGDRRYYDNFLYMFAMLALSGNYRIY"
     assembly_gap    4662..4793
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4794..5428)
                     /locus_tag="DHV81_00160"
     CDS             complement(<4794..5428)
                     /locus_tag="DHV81_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HCI92969.1"
                     /translation="MAGAYKTGKYRNIFKEYGYPEEEIQKRVEDTFNTIFYGNDDERF
                     YHEAGDDMGYMEDTGNHDVRTEGMSYGMMVCVQLDKKAEFDRLWKWVRTYMYIEDGPA
                     KNYFAWSCQTNGKRNADGPAPDGEEYFAMALFFASKRWGDGDGIFCYSKEAQNILREC
                     VHKGEPGHSGEPMWEPSNKLIKFVTNMDFSDPSYHLPHFYELFAQRAYEEDR"
     gene            complement(5453..7384)
                     /locus_tag="DHV81_00165"
     CDS             complement(5453..7384)
                     /locus_tag="DHV81_00165"
                     /inference="COORDINATES: protein motif:HMM:PF00756.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCI92970.1"
                     /translation="MDDLKNGALYIGTIPSSMDNNRCSVALEDDGSVTFYIYAPNANK
                     VEVAGMGGYFSSERIQLKPDMQGGFSANIKDFHWAMHYYFWYVDDVCITNPHAAISYG
                     CFAAINTFEVPKEGEDFYFVRDVPHGTVSLCKYTSQVNGHIKESYVYTPPGYESGDGR
                     YPVLYLQHGVGKNETGWVWQGKMNFIMDNLIADKKCVPMIIVASSGYAFKDNEYPVFF
                     PGDFDSELVNSIIPYIEENFKVKKGRNNRAVAGLSLGSGQATDIAARHPELFSAVGVF
                     SGVAIHLMKKIIGSPYRFEAVFMSAGDEEKEILLGINEMVKEFSRQGKNSTPKVYEGY
                     HEWHVWRKSFKDFAQMLFTWDDAELDDIDKAVPVRSKNIDFTTPVQADESMVFFDPVY
                     RQIQFENDEDGKPAGKYPDVIHGIRVTEDNSIEVNLFAPDAKSVSVVLENGTEELLYR
                     SKKNDGYWEKTIGNPAEGFNYVTFMVNGTPVVNPAAPVGFGYNRAVNFAEVPERNFSW
                     HELKKTEHGQIHIHYSCDGDGQVSMNYVYTPAGYGEDNCDTGRVCVLECAADERNFCW
                     IHQGKIANIMDNLSGEGRIKGIMIIMADSTISDDIIGNITAIYGIRDSARKEWFKKGD
                     NESWTSCRHRFLNFMCGIQ"
     gene            complement(7482..>8999)
                     /locus_tag="DHV81_00170"
     CDS             complement(7482..>8999)
                     /locus_tag="DHV81_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCI92971.1"
                     /translation="AFFSVEGDNIDPDNDIKQLIAEQIGAECEETWLVGQDKDDAINS
                     LIATGEYPDFVLGETALYEADALIPIDEYWDDYPNIKEYLSDEQWEKLRQPDGHIYWI
                     PPFGISHGDDVEMLHNGEAFWIQTRVLKWAGYPKITTIEQYFDLIESYVQANPTMPDG
                     TENIPFTMLCDDWRYFCLENVPQFLDGFPNDGSCMVDTETKKVMDYNVTDTAKRYFGK
                     LNEEFHKGIMDPGAFNATYDQYLDKLSTGAVLGMVDQWWQFYYAIDPVFKKQNLAQLG
                     CDYVPLPVTIDDGIHNRWHTNRMAEIDYSSGVSITTSCKDIEGAMKFVSDLLESDIIR
                     ERFWGEEGKDYSVDENGLFYLTNEQAEKKSDSSYQTAHMCSYSYFPRVEGMLDDGINA
                     FSMEYQPEEFYKNQPADIQECFDAYGVENYVELLGKNDAPGSWYPMYSYSDSIPTTSE
                     CGKVKNNLEAVKKRWLPQVIMADDFDAAWDQYMEEYNACNPQIYFDYLQQKVNEN"
     assembly_gap    9000..9115
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9304..9921
                     /locus_tag="DHV81_00175"
     CDS             9304..9921
                     /locus_tag="DHV81_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 domain-containing protein"
                     /protein_id="HCI92972.1"
                     /translation="MIIHEITEECFKKYGKVIDSIDLTELVSTMQTVEIPADVVYEPS
                     ISALEKLKCATELQQKTYGELPIQIGWCIGNNHKLNAVEYHRCSEVNIAATDAILILG
                     RQQDISVENTYDTSLMEAFRIPSGTAVELYATTLHYAPCNASAGGFLVAVVLPKGTNE
                     ALEHPHTGGEDALLAAKNKWLIGHPEGGLPEGSYIGLTGENLEIK"
     gene            complement(9989..10552)
                     /locus_tag="DHV81_00180"
     CDS             complement(9989..10552)
                     /locus_tag="DHV81_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCI92973.1"
                     /translation="MNGDERRKKIINILSSSKSPVAGVALAKELDVSRQVIVQDIALL
                     RANGAAIFSTNRGYLIQADKQYSRVLKVVHEDDEVEEELSTIVDAGGHVKDVFVYHKV
                     YGVIRANMDIKSRRDVRNYLEEIRTGKSSLLKNVTSGYHYHTICAESEEVLDAIQEEL
                     KQKGFLAKLQDYEPVDFWGDNEDEAKA"
     gene            complement(10612..>11382)
                     /gene="nadC"
                     /locus_tag="DHV81_00185"
     CDS             complement(10612..>11382)
                     /gene="nadC"
                     /locus_tag="DHV81_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCI92974.1"
                     /translation="STNSVMPHPQAGEVDLICKQDGIICGLQVFERVFTLLDADTKVE
                     FYVKDGDRVENKQLIGKVYGDIRVLLCGERTALNYLQRMSGIATYTSQVAALLEGTGI
                     RLLDTRKTTPNNRIFEKYSVRVGGGNNHRYNLSDGVLLKDNHIGAAGGVKEAIAMAKA
                     YAPFVRKIEVEVESFDMVKDAVEAGADIIMLDNMSTELLKQCIDYIDGRAEIEVSGNV
                     TKENIARIKGLGVDYVSSGALTHSAPIMDLSLKNLHAV"
BASE COUNT         2830 a         2802 c         1861 g         3641 t
ORIGIN      
        1 cagtcaccct ttgtctcatc cccctcatca tttacagcaa attcaataac tactatatca
       61 ggctgctgtc cgtctcttag tacatccctg tcaaatctga tcattcccag ctccgatggt
      121 gtgccaccca cacctgcttt tatgaatttc acattattct ttgcagaaaa acgcctctgg
      181 aaaagctgat aggatttgta tgcgtagcac tctgtattta taggtgtcgc acctgctccc
      241 tgtgtgatag atccaccgat gtatgcaagt gttacctctt ttccggctct tgtcttttcg
      301 atggcttttc ttatgcggta tacatttccc atattcataa gcgaatggct tatcatgctg
      361 cagtattcat cagagttcat atccacctct gtttcctcaa tatctgccgg agcctcatag
      421 ccgtcattca gatacagtct gacactcgct tttcccatga tatccggtgt attaaatatg
      481 aactttatct gtcccggtat atcatcatcc ggtgtccact tataatctga caggtatatc
      541 tttctctcca ttccatcgcc tgtcagcttt accttaagat tagtaccgcc tccgtacagg
      601 tcctgctttc cgtacatctg aaacacaaat tcgatctgtt cgttttgatt atctgtttcc
      661 acagatacac ctatgctgtg tacaagcctg ccaaaacctt ccaccgtctc taacagcccc
      721 agttcctctt tattttctat atttccggct atttgtgcac tgtttatcag tcttccgtcg
      781 ctttcatata taaaatgtat aatgcttcca tcttcctgta ctgagccgta tatatcctta
      841 tccttcatga tgtagaaata cggtcttttt ctttcgggat cctttggtgc ttttggccct
      901 gccatctttc tgcctccttg ttatgtcgat tttatcagct ataatgacta tatgatacaa
      961 aaactcccgg tgctttgcac cgggagaaat tatagatatt tgtcctgtct tttatagttt
     1021 atgactgctt tctgcttctt cttaatcttg aagcccctgc catcgaaact cctgcaagaa
     1081 acaacacgat aaatgctgcc gttgctgatt gatctgatgt ctttgcagct ttttttgccg
     1141 ccggcttgtt tgctaaaggc ttatttgtgc tgcctgttgc cggtttatcc gctgacggtt
     1201 tatttatagc cggcttgcct gtacccggtt tttctgtatt acctgttgat gaattatttg
     1261 tacccggttt ttctgtatta cctgttgatg gatcatctgt acccggttta ctattatcat
     1321 cagacacttc atcctttgcc tctgctgcaa gctctattgt tccgtatacc gatgatttag
     1381 accagcccat gcctgtttca tcattccagc ttaaggttcc gattcttgct cctgaagcat
     1441 cagcatcatt tatctggaac tcaagaccaa tcctgtcacc cttttttggc tttatgtctg
     1501 tccacttgaa ggctgcctca atcacatatc catccccagt cacttttgcg gcagattgta
     1561 cattttcctc gagacatttc tttccattga aagaatgctc attctcataa ttgatcctgt
     1621 actgcttatc atcgtcatca tatgactctg tcttggcatt attctcatcg ataaatacct
     1681 cgagtgaatc ctgctgatat gcctctccac cgtctttatt aagcaccgga tctttaactg
     1741 ttgcatatac ataaaggttt tcgtcatccc acaacacctt ggcatctgct gttaccttag
     1801 ctccaaggtt tattgtaaga ggaatagcca cagccttatc ccattcgctg tcggcttctc
     1861 cgtcaatctt taccgttgct tttgttgctt tttcaatgcc cggcttgaga gtagcctttg
     1921 cataatattt gctgcttgtc tcctgcattt ctttaaggtc attgaagctt acagctttat
     1981 cgttattcat aacttttaca tccatgccga tagtctttgc aacttttaaa tcagtcatag
     2041 gtactttgat ggtcgcacgg tatccaccat caacagcctg tgcctcagaa cgcttaactg
     2101 tcttctttac cggtgtcaca tctcctgctg agtttgtctc atccacatat actgtaactt
     2161 catccgtatc atttattgtg gcatccttta cgcttacaag tacatttaat ccatccttat
     2221 cccatgttgg aataaaagct gcctgtgtat catcatccga aaaactgtat tctgttccgg
     2281 acattgtatc acctttctgc tcagctacaa cgatatcctg tattaacggc tcaagctttg
     2341 tagcatctac ataagcccag tatgcaggct ttgccttgta ttttccatca aaaagcagtg
     2401 ggcactgctt tgatccgtca gctccgccac caacatttga ctgtgaatga agccatgaat
     2461 ttggctcaat aactccccac acagtgattc ctgctacatt tgtaccgttt ttctttaagt
     2521 cctttgcagc atcaaaaagc tgtttgtgac agtatgccat ctttgtatac tcgctctctt
     2581 ttgatgctgc tcctgatttg taagctgcgc ttgcctggaa atccagctct gtaagctgaa
     2641 cctttcctgc tgcttcggca tatttttttg ctactgtctt aaactgtgct gctgagaagc
     2701 tgtccacact gtaatgtgcc tgcataccaa atgcatcaag tcgtgttccc tgtgctgatt
     2761 ttacatcact tatgagcttt atgattcctt ctgacttgat attgtctgtc tctccgaaat
     2821 cattgtagta aagttctaca tcactaggcg catatttatt cgcatactta aatgcattga
     2881 ttatgaactc attgctcttg tatacatgcc accagctcga attgttccca tgacgtatct
     2941 catcaaggct ttctgccggg cttaccgtat ctgtacggta tgtattgccg ataacggcct
     3001 catttactac atcccagcca tagaaaagtc cgtcgtactt tttatttgct gcctctccga
     3061 agtagtggtc gaatacactt gagatatacc actcaagtct gcggttcata gtctctttat
     3121 ctacatatgg cttactgata tcataattct catggaagaa ccattccgga gtctgcgaat
     3181 gccatacgag aacatgacct cttatgcgta tcttcctgtc aggatgtgcc gcattccact
     3241 ccaaaatctt atctaccatc gcatcagctc ttgagaaatc aaggctgtcc cctgcgtcat
     3301 ttaccatcgg tacttcaaga tcctgcccat caaactgaac tgtttttgtt ttaaccgact
     3361 tctcaagctg ataattaaaa agtgcatccg gttttaattc atttccaaat gtaactgcat
     3421 taaaatgctt ctccacaagg tccatgagcg tattgtcctt aatctcatct aacatgatac
     3481 ctgttcctgc gattgcattt tctccaagag catcactgct tgtgacagat tttttccagt
     3541 cctcaatatt ctgctctatc tcaggtggtg tctttggtgt tgtatcagtt tccactgtca
     3601 cactaacgag gctgatagct gatcccgaac cggcattatc agtagtcata agaccaactg
     3661 ctaccatatc accttcacct gtcgggctta tcgagtattc atctgcgcct gtaagttcat
     3721 acttcgtatt tgcgccatca gcatcttcat ctccattctt gtagaccttt agtgaaactg
     3781 atcctttctg atccttcaca ttaaaggtca cagattttac agttgacata tcaacagtct
     3841 ccggcagtct ccatttaacc tgatcgtact gattgttaaa tgtcactttc cacacaccat
     3901 ctgtcagctc atagccggca tttccccagt ctactgtcaa tttatcagct gtgtatgtcg
     3961 ttttctttat attctgctga acttcatcag cgaatactaa cgactgcgcc tgatacgcag
     4021 gtataagctg tgtgacacct gtggcaagca tagtactgca taccgcaata catgctcctt
     4081 gtttcaatag ttttttcctt ttcaacccca tgtcctcctt acatgtgtat ttgtaattcc
     4141 aaaaacactc taccataaga gaggttttaa aatatgggac aaattataga atataacaag
     4201 tcaaattata tgtgcgataa ataaattcaa ataatagtaa gtctgcggta ccagaaccat
     4261 tgtatataaa aatcaataaa tcctgtaatt tcctgaaagt gctaacattg caaacatgta
     4321 aaggaaattg tcatagtatc tcctgtcacc tgtcctaagc ggagtgttcc agaacttttc
     4381 aacacattgc tttgcatatt ttccatctgc tgcgagtgaa gcttctgcat taacagcaat
     4441 gatcgctacc ggatgaagtg ccttctcatc aagcggtgta ccatctataa gaaatacacc
     4501 atcccaattg tccttctgat cctcacaata aaacttctga aggttttctg ccgttttaat
     4561 ctgccacgga tctgcatcaa accacagatg atccatcgct atattggcga tcgtgcggta
     4621 tgcatcactg taataccagt catgtcttcc aaatatttcc tnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggtcct
     4801 cctcataagc cctttgggca aaaagttcat aaaaatgcgg cagatggtat gaaggatcac
     4861 tgaaatccat atttgtgaca aactttatca gcttgttgga aggctcccac attggctctc
     4921 cgctgtgtcc cggctcaccc ttgtgtacac actcccttaa gatattttgt gcctctttgc
     4981 tgtagcagaa tataccatcg ccatctcccc atcttttaga ggcgaaaaat aatgccattg
     5041 caaaatactc ttcaccatca ggtgccggtc catcggcatt tcgctttccg tttgtctgac
     5101 atgaccacgc aaaataattt tttgccggac catcttctat gtacatatat gtgcgcaccc
     5161 atttccacag tctgtcaaac tctgcttttt tgtccagctg tacacatacc atcatgccat
     5221 atgacattcc ctcggttctc acatcatgat tgccggtatc ctccatatat cccatatcat
     5281 caccggcttc atgataaaaa cgttcatcat cattcccata gaatatcgtg ttaaaagtat
     5341 cctctactct tttttgaatt tcttcttccg gatacccata ctctttgaat atatttctgt
     5401 atttgcctgt cttatatgct cctgccattt ttctctcctt tttgtcgctg ttttactgta
     5461 tcccgcacat gaaatttaaa aatctgtgtc tgcatgatgt ccaggattca ttatctcctt
     5521 ttttgaacca ttctttccgt gcactgtccc taattccgta tatggctgtt atgttcccga
     5581 tgatatcatc agatatcgtg ctgtctgcca ttattatcat aattcctttt atcctgcctt
     5641 cacccgacag attatccatg atatttgcta tctttccctg atgtatccag caaaaatttc
     5701 tttcgtccgc agcacactca agcacacata ctctgccggt atcacaatta tcctctccat
     5761 aaccggctgg tgtataaaca taattcatac tgacctgtcc gtctccatca cacgaataat
     5821 ggatatgtat ctgaccatgc tctgttttct taagctcatg ccagctaaag ttcctctcag
     5881 gtacctcagc aaagtttaca gccctgttat atccgaaccc tacaggtgcc gcagggttta
     5941 ctacaggtgt tccatttacc ataaatgtca catagttaaa tccctctgcc ggattaccta
     6001 ttgttttttc ccaataacca tcatttttct ttgatctgta aagaagctcc tccgtgccat
     6061 tttcaagaac tacgctcacg ctttttgcat caggtgcaaa aagatttact tcaatgctgt
     6121 tatcctccgt cacacggata ccatgtatga catccggata tttgccagcc ggttttccat
     6181 cctcgtcatt ttcgaactgt atctgcctgt acaccggatc aaaaaatacc atgctctcat
     6241 ccgcctgtac cggtgtggta aaatcaatat tctttgatct gaccgggaca gctttatcaa
     6301 tatcatcaag ttctgcgtca tcccatgtaa aaagcatctg tgcgaaatcc ttaaagctct
     6361 ttctccatac atgccattca tgatagccct catatacttt tggtgtactg tttttcccct
     6421 gtcgtgaaaa ttcctttacc atctcattga ttccaagcag tatctctttt tcttcatctc
     6481 ctgccgacat aaagacagct tcaaaccgat atgggctgcc gatgattttt ttcataagat
     6541 gtattgcaac acctgaaaat actccaactg ctgaaaaaag ctccgggtgt cttgccgcta
     6601 tatccgtagc ctgtcctgaa ccaagtgaaa gtcctgccac agcacggttg tttcttcctt
     6661 ttttgacctt aaaattttcc tctatataag ggattatgct atttacaagc tcactgtcaa
     6721 aatctcccgg aaaaaataca gggtattcgt tatccttaaa tgcatagcca ctgcttgcaa
     6781 ctatgatcat cggcacacat tttttgtctg ctatcagatt gtccatgatg aaattcatct
     6841 tcccctgcca tacccatccg gtctcattct tcccgacccc atgctgtaaa tacaatacag
     6901 gataccttcc atctccgctc tcatatccgg gtggagtata cacatagctc tcctttatgt
     6961 gaccgtttac ctgtgaggta tatttgcaca gacttaccgt tccatgcggc acatccctga
     7021 caaaatagaa atcctcgccc tcttttggca cttcaaatgt gtttattgca gcaaaacagc
     7081 catatgatat agccgcatga ggatttgtta tacagacatc atctacatac cagaaataat
     7141 aatgcattgc ccagtggaaa tctttaatgt ttgccgaaaa tccaccctgc atatcaggct
     7201 ttaactgtat gcgttctgag ctgaaatagc ctcccattcc ggctacttct actttattgg
     7261 catttggcgc atagatataa aaggtaacgc ttccatcatc ctcaagtgcg accgagcatc
     7321 tgttattgtc cattgatgat ggtatcgtgc ctatatacaa agcaccattt tttaaatcat
     7381 ccaaaataaa atccccccat tcgtttattg atatatacaa gtgtaaacga acaggggctt
     7441 gttattctat taataaattg ccaaacagta ttcaaatatt gctaattttc atttaccttt
     7501 tgctgtaaat aatcaaaata tatctgtgga ttgcaggcat tgtactcctc catatactga
     7561 tcccaggcag catcaaaatc atctgccatt atcacctgtg gcagccacct cttcttaact
     7621 gcctcaagat tattttttac ttttccacat tcggatgtcg taggaatcga atctgaatat
     7681 gagtacatcg gataccatga acctggagca tcgtttttac cgagcagttc cacataattt
     7741 tccactccgt atgcatcaaa acattcctga atatctgcag gctgattttt atagaattct
     7801 tccggctgat actccattga aaacgcattt ataccatcat caagcattcc ctccactctt
     7861 ggaaagtatg agtacgaaca catatgcgct gtctgatatg agctgtcact ttttttctct
     7921 gcctgctcat tggtaaggta aaacaaaccg ttttcatcga ctgaatagtc cttgccctcc
     7981 tctccccaga acctttctct tatgatatca gattccagaa gatcactgac aaacttcatc
     8041 gcaccttcga tatctttaca ggaggtggta attgacactc ctgaagaata atctatttct
     8101 gccatcctgt ttgtatgcca tctgttgtga ataccatcat caatcgtgac aggaagagga
     8161 acataatcac acccaagctg tgccagattc tgttttttga atacaggatc tattgcataa
     8221 taaaactgcc accactggtc gaccatccca agcacagctc ctgttgacag cttatccaga
     8281 tattgatcat aggtggcatt aaacgccccg ggatccatta ttcccttatg gaactcttcg
     8341 ttaagctttc cgaaatatct tttggctgtg tcggtcacat tgtaatccat cacttttttg
     8401 gtttccgtgt ctaccataca gcttccatcg tttggaaatc cgtccagaaa ctgcggcaca
     8461 ttttcaaggc aaaaataccg ccagtcatca caaagcatcg taaatggtat attttctgtt
     8521 ccatccggca ttgttggatt tgcctgtaca tagctttcta tcaggtcaaa gtactgctct
     8581 atcgttgtaa tctttggata tccagcccat tttaatactc ttgtctgaat ccagaaggct
     8641 tcaccattat gcagcatctc cacatcatcc ccatgtgata ttccaaacgg tggaatccaa
     8701 tatatgtgtc catccggctg tcttaacttt tcccattgtt catcactgag atattctttt
     8761 atgttcggat aatcgtccca gtattcatct attggaatga gtgcatctgc ctcatacagc
     8821 gcagtctcac ccaatacaaa atccggatat tccccggttg ctatgagaga atttattgca
     8881 tcgtccttat cctgtccaac aagccaggtt tcctcacatt cagcaccgat ctgctctgct
     8941 atgagctgct ttatatcatt atccggatct atattatctc cttctacaga aaaaaaagcn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntattt
     9121 tcttttgttc agtctctgct ataaaattgc ataatagtca ttatattcca taaaaaatat
     9181 ttttacaaat cctaaaataa atatatctaa taactagggt ataaaacaat aatttatcct
     9241 gctgacattt tctctgtcat acattataat ttttacaatt ttaaaaatca ggaggaccat
     9301 ataatgatca tacatgaaat aacagaagaa tgttttaaga aatatggaaa ggtgatcgac
     9361 tctatagatc ttacagagct ggtcagcaca atgcagacag tagaaattcc tgccgatgtg
     9421 gtttatgagc catccatttc tgcacttgaa aagctaaaat gcgctacaga attacaacag
     9481 aaaacatacg gggagcttcc tatacagata ggctggtgca taggcaacaa tcataagctc
     9541 aatgctgttg aatatcacag atgctctgag gtcaatattg ccgcaactga tgccatactg
     9601 atactcggcc gacagcagga tatctccgtg gaaaatacat atgatacctc tttgatggaa
     9661 gctttcagga ttccttccgg aacagcagtc gagctttatg ccacaacact gcactacgca
     9721 ccatgtaatg catctgctgg cggatttctg gtagccgttg tacttccaaa aggcacaaat
     9781 gaagccctgg aacatccaca caccggcgga gaagatgctc ttcttgcagc taaaaacaaa
     9841 tggcttatcg gacatcctga aggtggactt cctgagggat catacatagg gcttacaggt
     9901 gaaaatctgg aaattaaata aaaagcaaaa aacagcccgg gctttcaccc gggcaattat
     9961 tatattacgc tttaacttta tgataaaatt aagcctttgc ctcgtcctcg ttgtctcccc
    10021 agaaatccac cggctcatag tcctgcagct ttgcaaggaa gcccttctgc ttaagctcct
    10081 cctgtatggc atcaagcacc tcttcactct ccgcacatat tgtgtgatag tgataacctg
    10141 aagttacatt tttaagaagg ctggactttc ctgtgcggat ttcctccagg taatttctga
    10201 catcacgtct ggacttgatg tccatattag cgcggatcac tccgtagacc ttgtggtata
    10261 caaatacgtc cttcacatgg cctccggcat caactattgt tgaaagctcc tcctctactt
    10321 catcatcctc gtgaaccacc tttaatactc tcgaatactg cttatccgcc tgtatcagat
    10381 atcctctgtt tgtcgaaaaa atcgcggctc cgtttgctcg aagcagtgct atgtcctgca
    10441 caatcacctg acgactcaca tcaagctcct ttgcaagcgc aactcccgcc acaggcgact
    10501 ttgacgagga tagaatattt attatttttt ttcttctctc atcaccgttc attttcgttc
    10561 ctttctgtaa aacattactg tcacagcata gtgcgaccgg caatataaaa tctatactgc
    10621 atgtagattt ttaagtgata gatccataat aggcgctgaa tgtgtgagcg caccacttga
    10681 tacatagtct acaccaagac cctttattct tgcaatattt tctttggtaa catttcccga
    10741 aacctcgatt tcggctctgc cgtcaatgta gtcgatgcac tgctttaaaa gctctgtgga
    10801 catgttgtcg agcatgataa tgtcggcacc ggcttcaacc gcatccttca ccatatcaaa
    10861 gctctctacc tcaacctcaa tctttctgac aaacggagca taggcctttg ccatggcgat
    10921 tgcctctttt acgccgccag ctgctccgat gtggttatcc ttaagaagca ctccatccga
    10981 gagattgtat ctgtggttgt ttccaccacc gactctgacc gagtattttt caaaaattct
    11041 attgtttgga gttgtctttc tggtatccaa aagcctgata cctgtgccct ctaagagcgc
    11101 tgcaacctga ctggtataag tggcaattcc actcatacgc tgtaaataat ttagcgcagt
    11161 tctctcaccg cacaagagta ctctgatatc tccgtaaacc ttgccgataa gctgcttatt
    11221 ttctacccta tcgccatcct ttacataaaa ctctaccttt gtatctgcat caagcagtgt
    11281 gaaaactctc tcaaaaacct gcagtccaca gatgattccg tcctgtttgc agattaaatc
    11341 cacctctcct gcctgtggat gtggcatcac gctgtttgtg ga
//
LOCUS       DPUF01000007           10205 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8518, whole
            genome shotgun sequence.
ACCESSION   DPUF01000007 DPUF01000000
VERSION     DPUF01000007.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8518"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            296..2107
                     /locus_tag="DHV81_00190"
     CDS             296..2107
                     /locus_tag="DHV81_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI92975.1"
                     /translation="MQGEKFTLMQYNVNAILGFIEAGQFVIPEIQRPFVWKRAQVRDL
                     IDSLYNGYPTGYIITWKNPDVKTKDGTVANGKHVLIDGQQRITALMASIAGLEVLDED
                     FNRDRVKIAFNPLATDSDKRFAVQDASHLKDKKWIPDISEVFKNDFDPFEFVPKYCNN
                     NLGVKPNEVNNAIMKLRGIANRQIGVIELDHSLDIDEVTEIFIRINSKGTALSQSDFV
                     MSKMAADTVHGGNILRKVVDYFCHLAVKPDFYPQMIKDLEFEKTEFASKIKWLAKDNE
                     DIYNPDYNDMLRVAFMYSFNRAKLSDLVSLLSGRDFETREFKEEIVEDSYNKLCEGIE
                     VFINEHNFEQFVLAIKGAGFKSSKQLNSQMTLDFAYMLYLKLSKDSSIPHDQVKHHVQ
                     KWFVLSTLTGRYVGSPESVMSRDIRLINEKGFINFFYEIEASVLSDTFWDISLPQNLE
                     TTSTNSPAFNTFLAAQINLNCNSLFMHGTMISDLINISGDVHHIFPKAYLKNNGIDTK
                     GRYNQVANFTYLDTQVNKAISDDAPNIYFQSALEQCDKKNIAFGNIFERDALMKNLSE
                     NAIPESIVSMTVENYDDFLADRRKLMAKLMMRYYKGL"
     gene            2337..2594
                     /locus_tag="DHV81_00195"
     CDS             2337..2594
                     /locus_tag="DHV81_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCI92976.1"
                     /translation="MELAKVTSKGQITIPIEIRKKLGIKNGDKILFVEESGRVYMMNS
                     SMDALREAQRSFAGEAERLGLKDDDDVMEMIKSLRQESVVK"
     gene            2595..3002
                     /locus_tag="DHV81_00200"
     CDS             2595..3002
                     /locus_tag="DHV81_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HCI92977.1"
                     /translation="MRIMLDTNVLISALLFPGTKMDAMMNYIFTNHQLVLSSYVVDEL
                     KNVVRRKFPGKEMIINKLLMMMSFEYVYTPGSMESGLFNIRDIKDYPVLYTAIIEDVD
                     ILVTGDKDFSDIDVEKPKIMSPTQFMENFLEEW"
     gene            complement(3123..4181)
                     /locus_tag="DHV81_00205"
     CDS             complement(3123..4181)
                     /locus_tag="DHV81_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HCI92978.1"
                     /translation="MSITAKELAKQLNLSEAAISMALNNKPGVSTLTRKRVLEAAASA
                     GYDFSRISNTNEAPASNGTLYFVIYRKNGAVVPNSPVYTHTEHGVLVQDVPFFSQLSE
                     GIDLGCRHCHYYLNISYIYENDDIEALLSEWKRLGAKGILLLGTEMEEHDIKPFTRCG
                     LPVVLIDNYFEALNLDCVTINNLQGAYLATDYLIKQTHAQPGYLHSAYSITGFEERAD
                     GFYKAIRKNGMSTSRSVVHHLSPSVDGAYCDMKAVIKSGDELVRCYFADNDLIAAGAM
                     RALSEEGYRIPEDISVIGFDDMPMCTYITPPLSTVHVPKQYMGEIAVKRLAEIINSTS
                     ASHVKIEISTEIIKRKSC"
     gene            4380..5864
                     /locus_tag="DHV81_00210"
     CDS             4380..5864
                     /locus_tag="DHV81_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HCI92979.1"
                     /translation="MNNIPKMKIGIVAVSRDCFPESLSVNRRKALVDAYAAKYDAADI
                     YECPVCIVESEIHMVQALEDIKAAGCNALCVYLGNFGPEISETLLAKHFDGPKMFVAA
                     AEETQENLIQGRGDAYCGMLNASYNLALRNIGAYIPEYPVGDADDCADMIHEFLPIAR
                     AIYGLNNLKIISFGPRPLNFLACNAPIKQLYNVGVEIEENSELDLFEAFNKHAGDERI
                     PDVVKDMERELGEGNKKPEILEKLAQYELTLLDWVDEHKGYRKYVAIAGKCWPAFQTQ
                     FGFVPCYVNSRLTGRGIPVSCEVDIYGVLSEFIGTCVSEDAVTLLDINNSVPKDMYKE
                     SIEGRFDYKHTDTFMGFHCGNTCSSKVCNPQMKYQMIMARSLPIEVTNGTLEGDIVPG
                     DITFYRLQSTADCKIRAYVAQGEVLPVATRSFGSIGVFAIKEMGRFYRHVLIEKNYPH
                     HGAVAFGHYGKALFEVFKYIGVPVEDINYNQPASLPYKTENPFC"
     gene            5923..7407
                     /locus_tag="DHV81_00215"
                     /pseudo
     CDS             5923..7407
                     /locus_tag="DHV81_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517286.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     assembly_gap    6325..6426
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7623..8951
                     /locus_tag="DHV81_00220"
     CDS             7623..8951
                     /locus_tag="DHV81_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCI92980.1"
                     /translation="MDLFDYMREQNKETSGPLASRMRPTTLDEVVGQQHIVGKDKLLY
                     RAIKADKLSSIIFYGPPGTGKTTLAKVIAHTTSAEFMQINATSAGKKDMEEVVEQAKN
                     NQGMYQKKTILFIDEIHRFNKGQQDYLLPFVEDGTIILIGATTENPYFEVNGALLSRS
                     VIFELKKLSTDDIKVLLKRALTDTEKGMGAYNAQIDDDALDFLADVSNGDARAALTAI
                     ELGVLTTDRSGDGIIHITIDVASECIQKRVISYDKKGDNHYDTISAFIKSMRGSDPDA
                     AVYYLARMLYAGEDVKFIARRIMILASEDIGNADPMAMVVAASAAAEVERVGMPEAQI
                     ILSQAVTYMACAPKSNASYNAISAAMSCVKQTKTPAVPAHLQDAHYGAAEKLGHGVGY
                     KYAHDYPNHYVKQQYLPDGLTDRVFYKPTDIGYEAKIKAHMDELHRDCDT"
     gene            9036..>10205
                     /locus_tag="DHV81_00225"
     CDS             9036..>10205
                     /locus_tag="DHV81_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCI92981.1"
                     /translation="MKKYTEMTKDELMQEKTALEAEYEKIKNLGLSLDMSRGKPGADQ
                     LDLSLPMLDVLKSDSDMKSESGLDVRNYACLDGIPEAKALIAGMVGAKPEQAIVYGNS
                     SLNIMYDQVSRAFIFGLCGNTPWCKLDKVKFLCPVPGYDRHFAITEEFGVEMINIPMT
                     EDGPDMDMVEKYVNNDASVKGIWCVPKYSNPQGVVYSDETVERFAKLKPAADDFRIFW
                     DNAYTVHHLYDDKQAEIPNILELCEKEGNPDMVFEFCSTSKVTFPGAGLAGICTSEKN
                     REDIKKRLTIQTIGHDKINQLRHARFFKDVDGIKAHMAKHAALIRPKFELVENILTDE
                     IASRGIGTWVKPLGGYFFGFEALSGCAKEIVAKCKEAGVTMTGAGSPFPYKKDPDD"
BASE COUNT         3163 a         1701 c         2431 g         2808 t
ORIGIN      
        1 gatggtgtga gagcaggaga tactatcctc atagatgtga aggatggaca gcttagtgca
       61 tccgcagtgc agaaaaatgc ataaaaatga agatatattt gtaaaacagg cgcaggaatt
      121 ttctccgcgc ctgttttatt ttgcgctttg gcatggagga cagacctctg ataaaggaga
      181 aaaaaatcct tctctttttt aattgtgtta ttatttgaaa aaatatataa ttaatgtaag
      241 caactagaaa ataagtcgaa aattttaata atcaaatgtt tgaggtgata agattatgca
      301 aggagaaaag tttacattaa tgcaatataa tgttaatgca atattgggct ttattgaagc
      361 agggcaattt gttattcctg agattcagag accatttgta tggaaaagag ctcaggtaag
      421 ggatttgatt gattctttgt ataatggata ccctacaggg tatattatta catggaagaa
      481 tccggatgtt aagacaaagg atggaacagt agcaaatgga aagcatgtat taattgatgg
      541 acaacagaga attacagcat tgatggcatc aatagcaggt cttgaagtgt tagatgaaga
      601 ttttaatagg gatagggtta aaattgcatt taatccgctt gcaaccgatt cagataagag
      661 atttgcagta caagatgcat ctcacttaaa ggataaaaaa tggattccag atatttcaga
      721 agtatttaaa aatgattttg atccatttga gtttgtccct aagtattgta ataataattt
      781 gggtgtgaaa cccaatgaag tgaataatgc aattatgaag ctgagaggga tagcaaatag
      841 acagattgga gttatagagc ttgatcattc tcttgatatt gacgaagtaa cggaaatatt
      901 tattcgtatt aattctaagg gaactgcatt aagccagtct gattttgtta tgtccaaaat
      961 ggctgcagat acggttcatg gaggaaatat attgcgtaag gttgttgatt atttttgcca
     1021 tttagctgta aaaccggatt tttaccccca aatgattaaa gatttagaat ttgaaaaaac
     1081 agaatttgca tctaaaatta aatggctagc taaagataat gaggatattt ataatcctga
     1141 ttataatgat atgcttcgtg tcgctttcat gtatagtttt aatcgtgcaa agctctccga
     1201 tttagttagc ttgctttcag gtagagattt tgaaacacgt gagtttaagg aagaaattgt
     1261 agaagattca tataataaac tttgtgaagg tatcgaagta tttatcaatg aacataattt
     1321 tgaacaattt gtattggcta ttaaaggtgc tggatttaaa tctagtaagc agctcaattc
     1381 tcagatgaca cttgattttg catatatgtt atatcttaaa ctatcaaaag attcttcaat
     1441 accacatgat caagtaaagc atcatgtgca aaaatggttt gttctttcaa cacttacagg
     1501 aagatatgtt ggatcgcctg agtctgtaat gagtagggat attagattaa taaatgaaaa
     1561 aggttttatt aacttctttt atgagattga agcatcggtt ctttcagata cattttggga
     1621 tatatctttg ccacagaatc tagagacaac gtcaactaat agtccggcat ttaatacgtt
     1681 tcttgcagca cagattaatc ttaattgcaa ttcgttattt atgcatggaa caatgatttc
     1741 agatttaatt aatatatcag gagatgtaca tcatatattt cctaaggcat atttgaaaaa
     1801 taatggcatt gatacaaagg ggaggtataa tcaggttgca aattttacgt atcttgatac
     1861 tcaagtgaat aaagctataa gtgatgatgc cccgaatata tattttcaaa gtgcattgga
     1921 acagtgtgac aagaagaaca ttgcatttgg aaatatattt gaaagagatg ctcttatgaa
     1981 gaatcttagt gagaatgcaa ttccagaaag cattgtatca atgactgtag agaattatga
     2041 tgatttcttg gctgatagga gaaagctgat ggcaaaattg atgatgagat attacaaggg
     2101 actatagatt attctattgt tttagtaaat aaaacagtaa gggagaagaa gatgagattt
     2161 tatggatata aagcaactaa ttgggaaaac tacagactat gataaaaaga ttgcacttga
     2221 gagaaagaac aaccaagttt ttatcggtgg attgtggttg ataaacttat gaaaaatgga
     2281 tataattata ttgtaagaaa agtaaggaat ccattacttg ggaaggaggt ttttgtatgg
     2341 aattagcaaa ggtaacatca aaagggcaga taacaatacc tattgaaatt agaaaaaaac
     2401 ttggaattaa gaatggcgat aagatattgt ttgtggaaga gtctggaaga gtgtatatga
     2461 tgaattcatc tatggatgca cttcgtgaag cacagagatc atttgcagga gaggcagaaa
     2521 gactcggact taaagacgat gatgatgtaa tggaaatgat aaaatctctt aggcaggaaa
     2581 gtgtggtgaa gtagatgaga attatgcttg ataccaatgt attgatatcg gcattacttt
     2641 ttccggggac taagatggat gcaatgatga actatatatt tactaaccat cagcttgtgt
     2701 tgtcatctta cgttgtagat gagttaaaaa atgtagtcag gagaaagttt cccggaaaag
     2761 aaatgataat caacaaatta cttatgatga tgagctttga gtatgtttac acacctggta
     2821 gtatggaaag tggactattt aatatcagag atatcaaaga ttatccagta ctttatacgg
     2881 ctattattga ggatgttgat attcttgtga caggagataa agatttttca gatatagatg
     2941 tcgagaagcc gaaaattatg tctccaacac agtttatgga gaatttttta gaagaatggt
     3001 aaaaatgttc ggggattata aaaacactca aaatcagcta tacctgatat ggtatagctg
     3061 atttttttga aaattaatat gactccgttt gttttgtaga ctggcagaat gcagaagagt
     3121 ctttaacagc tctttctctt tataatttct gtacttattt caatttttac atggcttgca
     3181 gaggtagaat ttattatctc tgcaagcctt tttactgcaa tttcacccat gtactgctta
     3241 ggcacatgca cagtcgaaag aggtggtgtg atgtaggtac acattggcat atcatcaaag
     3301 cctattactg atatgtcttc agggatacga tagccttcct ctgaaagtgc acgcatagct
     3361 ccggcagcaa tcaggtcatt gtcggcaaag tagcatcgga caagctcatc accgcttttt
     3421 ataactgcct tcatatcaca gtaagcaccg tcaactgaag gggagaggtg atgtacgact
     3481 gagcgcgagg ttgacatacc gtttttgcgt attgctttat agaagccgtc ggctctttcc
     3541 tcaaagcctg ttatactgta tgcggaatgc agatatccgg gctgtgcatg tgtttgtttg
     3601 atcaggtagt ctgtggcaag ataggcaccc tgaaggttat ttatagtcac gcaatcgagg
     3661 tttaaggctt cgaaatagtt gtctattagc acaacaggca gaccgcacct tgtaaagggc
     3721 tttatgtcat gctcttccat ctcggttcca agcagcagga taccctttgc tccaagcctt
     3781 ttccactcag atagcaaagc ctcaatatcg tcattttcgt atatgtagct gatattcagg
     3841 tagtagtggc agtgcctgca gcccaaatca ataccctctg acagctgtga aaagaatgga
     3901 acatcctgta ccaaaacgcc gtgctctgta tgtgtgtaaa caggagaatt aggaactaca
     3961 gcgccattct ttctgtatat tacaaaatat aatgtgccat tggaggccgg agcttcattg
     4021 gtatttgata ttcttgaaaa atcatatccg gcagaggcag cagcttcgag cacacgtttt
     4081 ctggtgagtg tgctgacacc gggcttatta ttaagtgcca tcgatatagc tgcttcagaa
     4141 agattaagtt gtttggcaag ttcttttgct gtgatagaca tggcatagct ccttatgttt
     4201 tatttgcctt attataacat gacaatatag tattaacatt ataatttagt aaattaagtt
     4261 gaaaaataca ctaaatttac taaattatag gtatcaatta agctggaccg gatatatact
     4321 tagcacataa gaacaattaa tctgtccgat ggggcatatg agagaaggag atttacacta
     4381 tgaacaatat tccaaaaatg aaaatcggta tagttgcggt aagccgtgac tgttttccgg
     4441 aaagtctgtc agtaaacaga agaaaagcac ttgtcgatgc atacgctgca aagtatgatg
     4501 cagcagacat atacgagtgt cctgtatgta tcgtggagag cgaaatacat atggtacagg
     4561 cacttgagga cataaaggca gcaggctgca atgcactttg tgtttacctt ggaaactttg
     4621 gaccggagat ttcagagact cttcttgcaa agcactttga cggaccaaag atgtttgttg
     4681 cagcagcaga ggagacacag gagaacttaa ttcagggacg tggagatgca tactgtggta
     4741 tgttaaacgc aagctacaat ctggcactca gaaatatagg agcgtatatt ccggagtatc
     4801 ctgtgggcga tgcagatgac tgcgcagata tgatacatga attcctacca atagcaaggg
     4861 ctatatacgg acttaataat cttaagatta tttcattcgg accaagacca ctcaatttcc
     4921 ttgcatgcaa tgcgccaatc aagcagctct acaacgtggg tgttgagatt gaggagaatt
     4981 cagagcttga tttgtttgag gcatttaaca agcatgccgg tgatgagaga ataccggatg
     5041 tggtaaagga catggaaagg gagcttggcg agggcaacaa aaagcctgag attcttgaga
     5101 agcttgcaca gtatgagctc acacttttag actgggttga tgagcataag ggctacagaa
     5161 agtatgtggc aatcgcagga aaatgctggc ctgccttcca gacacagttt ggatttgtac
     5221 catgctatgt aaacagccgt cttacaggaa gaggtatccc ggtttcatgt gaggtggata
     5281 tctatggagt gctcagtgag ttcatcggaa cctgcgtcag cgaggatgcg gtaacacttc
     5341 tcgacatcaa caactcagtg ccgaaggata tgtacaagga gtcaatcgag ggcagatttg
     5401 actacaagca tacagataca tttatgggct tccactgtgg aaacacctgt tcaagcaagg
     5461 tttgcaaccc acagatgaag tatcagatga ttatggcaag aagccttcct atagaggtga
     5521 caaacggaac tctcgaggga gatatagtgc cgggagatat tacattttac cgtctccagt
     5581 ctacagcaga ctgtaagatt cgcgcttatg tggcacaggg tgaggtgctt cctgttgcaa
     5641 cacgctcatt cggcagtatc ggagtgtttg cgataaagga gatgggacgt ttctaccgtc
     5701 atgtgcttat tgagaagaac tatcctcatc acggagctgt agccttcggt cattacggaa
     5761 aagccctttt tgaggtattt aaatatattg gcgtgccagt tgaggatatt aactacaatc
     5821 agccggcatc actgccatac aagacagaaa atcctttctg ttagtgggtt ctgaatagtt
     5881 acgttatctg atataaacat attacgtgaa tggagaatag atatgctata tataggtgtg
     5941 gatttaggca caagtgcagt aaagcttctg ctcatggatg agagcggaaa ggtattaaat
     6001 atagtctcaa aggagtatcc aatcagtttt ccaaagccgg gctggtcaga gcagaatcca
     6061 tgtgactggt gggagcagac cgtggctggg cttatagagc tgactaaaga ttttgacagg
     6121 agcgctgttg cgggaatcag cttcggcggt cagatgcatg gacttgttgt attggatgaa
     6181 aacgacaatg taataagacc tgctatactt tggaatgacg gaagaacaca gaaggagaca
     6241 gactatttaa ataatgtaat aggaaaagaa aagctgtctg agctcaccgg aaatatagcg
     6301 tttgccggat ttacagcgcc aaagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnatga caggtgtatt tagtaccgat tattctgatg catcgggaat gcttcttctg
     6481 gatgtcaaaa ataagtgctg gtcaaaggag atgcttgaca tatgcgatgt aagagaggag
     6541 tggcttcctg ggctttatga gagcagtgaa aagacaggca ctctgaaagc tgatgtggca
     6601 tgtgagcttg gctttcctaa agactgcata gttgcagccg gggcaggaga taatgcggct
     6661 gcagctatag gcacaggtac agtcggagcc ggaaagtgca acctctctct tggaacaagc
     6721 ggtacacttt tcattacaag tgatgagttc agagtggatt cgcacaatgc gcttcattcg
     6781 tttgatcatg ctgacggcgg ctatcatctg atgggctgca tgctctcggc tgcaagctgc
     6841 aacaactggt ggatgaagga tatccttcat tccgatgact ttgtgaatga gcagaagcct
     6901 cttgaggagg atggcagact tggtgagaat catgtgtatt ttctgcctta tctgatggga
     6961 gagcgctctc cacacaatga tccgtctgca agggcagcgt ttatcgggat gagcatggat
     7021 actgacagag cagacatgct gcaggctgtg tttgagggtg tggcatatgg tcttcgagat
     7081 tcacttgagg tggcaaagag ccttggtgtg gcaccaaaaa gcacaacaat ctgtggtggc
     7141 ggtgcaaaga gtgtgctttg gagaaagata gttgcaaacg tcatgaatat gcaggttgac
     7201 acggtagagg tcgaggaagg accggcatac ggcggtgcaa tactggccgc agttgcaaac
     7261 ggctgctttg aaaatgtgga ggctgcggca gcggctattg tgagaaagaa ggatacaacc
     7321 atgccaagtc cacagcttgt cgaaaaatat gagaagggct atgcaaaatt taagcagttt
     7381 tatcctgcat taaagggcaa atattagtca tttcgtagaa aattaaattt tgtgttgtaa
     7441 agtggtgcaa attcatgtat cttattataa ggaaatgaat tttgcaccac ttttttatgc
     7501 ataattagca cttataaaac acgtaattgc cgattatatc aatgcaaaat gaattttttg
     7561 ctaaaagaaa ttgcaaaatg atataacagg tatatcaaat atgcatttca ggcggggatg
     7621 atatggattt atttgattac atgagagaac agaataagga gacaagcggt cccttagctt
     7681 caaggatgcg tccgacaaca cttgatgagg tggtcgggca gcagcacata gttgggaagg
     7741 acaagctttt gtatcgtgcg attaaagccg ataagctcag ctccatcatc ttttacgggc
     7801 cgccgggcac cggaaagact actttggcaa aggtcatagc ccacactact agtgctgagt
     7861 ttatgcagat taatgcaacc tctgcaggca agaaggacat ggaagaggtc gtggagcagg
     7921 cgaagaataa tcagggaatg tatcagaaga agacgatact ttttatcgac gagatacatc
     7981 gcttcaacaa ggggcagcag gattatctgc ttccgtttgt ggaggatggc accattattc
     8041 tgataggagc taccactgag aatccttact ttgaggtaaa cggtgctttg ctttcacgct
     8101 cagttatttt tgagcttaaa aagctgtcaa cggatgatat aaaggtactt cttaagagag
     8161 cactgactga cacagaaaag ggcatgggtg catacaatgc acagatagat gatgatgcac
     8221 ttgatttcct tgcagatgtg tcaaacggag atgcgagagc ggcgctcaca gcgattgagc
     8281 ttggtgtact gaccacggac aggtcaggcg atggcatcat tcatatcact atcgatgtgg
     8341 cgagcgaatg tatacaaaaa agagttatct cctatgataa gaagggggat aaccattacg
     8401 ataccatctc tgcttttata aagagcatgc gcggctcaga cccggatgcg gcggtctatt
     8461 atctggcgag gatgctttat gccggagaag atgtgaaatt tatcgcaaga cgaatcatga
     8521 tacttgcatc ggaggatata ggcaatgcag acccgatggc tatggttgta gcggcatcgg
     8581 cggcggcaga ggtcgagcgc gttggaatgc cggaggcaca gattattctg tcacaggctg
     8641 tcacatacat ggcatgtgcg ccaaagagca atgcatcata taatgcgata tcggcagcaa
     8701 tgagctgcgt gaagcagact aagacaccgg ctgtaccggc acatctgcag gatgcccatt
     8761 acggtgcggc agaaaagctc gggcacggag tcgggtacaa atacgcacac gactatccaa
     8821 atcactatgt gaagcagcag tatctgccgg atggccttac agacagagtg ttttacaagc
     8881 ctacggatat aggctatgag gcaaagataa aagcacacat ggatgagctt cacagagact
     8941 gtgatacatg aaagtgcata gatgaagctt gaataattgc aaatagttga taataacata
     9001 aatatttaga aaaatcagac caggaggatc tttaaatgaa aaagtataca gaaatgacaa
     9061 aggacgagtt aatgcaggaa aagacagcac ttgaggctga gtacgagaag attaagaatc
     9121 ttggcttaag ccttgacatg tcgcgtggaa agccgggagc agaccagctt gacctctcac
     9181 tccctatgct cgatgtcttg aaatcagaca gtgatatgaa aagtgaatca ggacttgatg
     9241 tgagaaacta tgcatgtctg gacggaattc ctgaggcaaa ggcccttatc gcaggcatgg
     9301 taggagcaaa gcctgagcag gctatagtat atggaaactc aagtctcaat atcatgtatg
     9361 atcaggtttc acgtgcattt atctttggtc tttgcggcaa tacaccatgg tgcaagcttg
     9421 acaaggtgaa gttcctctgt cctgtaccgg gctatgacag gcactttgca atcacagagg
     9481 agtttggagt tgagatgata aacattccta tgactgagga tggtcctgac atggatatgg
     9541 ttgagaaata tgtcaataac gatgcctctg taaagggaat ctggtgtgtt ccaaaatact
     9601 ctaacccaca gggtgttgta tactctgatg agacagttga gcgctttgca aagcttaagc
     9661 ctgcagcaga tgattttaga atcttctggg acaatgcata cacagttcat catctctatg
     9721 atgataagca ggcagagatt ccaaatatcc ttgagctctg cgagaaagag ggaaaccctg
     9781 atatggtatt tgagttctgc tcaacaagta aggtaacatt ccctggtgca ggcttggcag
     9841 gtatatgcac atctgagaaa aaccgtgagg atatcaagaa gcgccttact atacagacaa
     9901 tcggacacga caagattaat cagcttcgtc atgcgagatt ctttaaggat gtagatggta
     9961 tcaaggctca tatggcaaag catgcagctc ttatcagacc taaattcgag ctggttgaga
    10021 atatcctcac agatgaaatt gcttcaagag gcatcggaac atgggttaag ccgcttggtg
    10081 gatacttctt cggatttgag gctctttcag gctgtgcaaa ggaaatcgtt gcaaagtgca
    10141 aggaagctgg tgtcacaatg acaggtgcag gctctccttt cccttacaag aaggatccgg
    10201 atgac
//
LOCUS       DPUF01000008            6168 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_18771, whole
            genome shotgun sequence.
ACCESSION   DPUF01000008 DPUF01000000
VERSION     DPUF01000008.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_18771"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            115..4682
                     /locus_tag="DHV81_00230"
                     /pseudo
     CDS             115..4682
                     /locus_tag="DHV81_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743108.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
     assembly_gap    3303..3329
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4697..>6168
                     /locus_tag="DHV81_00235"
     CDS             4697..>6168
                     /locus_tag="DHV81_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HCI92982.1"
                     /translation="MGKPTGFMEYDRCEAASVEPKERIKNFNEFHVFLSKENQQKQAA
                     RCMDCGVPFCQSGMTIAGMTSGCPLHNLVPEWNDLVYTGNWEQAYNRLHKTNNFPEFT
                     SRVCPALCEKACTCGHWGSPVSVRDNEHGVIETAYANGYAAPKPPKVRTGKKVAIIGS
                     GPSGLAAADQLNQRGHEVTVYEREDRVGGLLMYGIPNMKLDKSIIDRKVNIMKEEGIH
                     FVTGCDVGKDIKPAELYKKYDRVILCCGAKNPRDIKADGRDAKGIYFAVDYLTQNTKS
                     LLNSNLTDGKFVSAKGKNVVIIGGGDTGNDCVGTAIRQGAKSVTQLEMMPCPPTERAA
                     NNPWPEWPKVLKTDYGQEEAIAVFGHDPRIYKTTVKEFHKDKNGNLKELTIVSLESKK
                     DEKTGRFMMVPVEGSEKKLPAELVLIAAGFLGTESYVAKAFGVELNARTNVSTQEGHY
                     ATNVKNVYVAGDNHRGQSLVVWAIREGREAAREVDESLMG"
BASE COUNT         1927 a         1190 c         1561 g         1463 t
ORIGIN      
        1 cgagaaaaaa actatttaca ttgagctttc atagttgtaa aatcataaac aggaaatgat
       61 tcacttctta ataagttatc cgttaacaaa tttacactag gaaaggaaga acctatgagt
      121 aaacaatgga atgaaagcac agccacagct aagggattat atgatcctcg ctacgagcat
      181 gataactgtg gaattggtgc agtagtaaat atcaaaggta tcaaaacaca tgagacagtc
      241 gagaatgctt taaagattgt tgagaactta aagcacagag ccggaaaaga cgctgatggt
      301 aagacgggag acggtgtcgg catcctgctt cagatttctc ataagttttt taagaaggtt
      361 acaaagccac ttggaataga tcttggagac gaaagagatt acggtgtggg aatgtttttc
      421 ttccctaatg atgagttcaa gactattcag gcaaagaaga tgcttgaggt tatcgtggag
      481 aaggaaggcc ttgaatttct cggatggaga gaagtgccta cagagcctga taagctttct
      541 aaaaaagcca gagattgtat gccatgcatc atgcagtgct ttgtaaaaag acctgaggat
      601 gttgagagag gacttgagtt tgacagaaag ctgtatgttg caagacgtgt tttcgagcag
      661 tcaaacgaca atacctatgt ggtttccttc tcaagcagaa caatcgtata taagggaatg
      721 ttccttgtag agcagctcag acagttcttc atggatttac aggatcctga ttatgagtca
      781 gcaatcgcca cagtacattc aagattctct acaaatacaa acccaagctg ggagagatct
      841 catccaaaca gatttatcgt acacaatggt gagattaata ctattcttgg aaactctgac
      901 aagatgtcag cacgtgagga gaacatggag tctcctaagc ttaaaaaaga gttccagaag
      961 gttctgccgg ttatcaatgc ggcaggatct gattcagcaa tgcttgataa tgcgcttgaa
     1021 ttcctggtaa tgagtggtat ggagcttcca cttgccgtta tgataatgat tcctgagcca
     1081 tgggcaaaca attcaatcat gacccagaag aagaaggatt tctaccagta ttacgctaca
     1141 atgatggagc catgggatgg tccggcttca atcgtattct cagatggtga tctggtggga
     1201 gcagtgcttg acagaaacgg acttcgtcca tcacgttact atgtgacaga tgatgattat
     1261 cttattcttt catctgaggt cggagtactt gagattgacc ctacaaagat tgtgaaaaag
     1321 gatcgtctcc gtccgggaaa gatgctcctt gtggatactg tcgcaggaaa aatcatagat
     1381 gatgatgagc tcaaggagag atatgcagac aagcagcctt atggtgagtg gattgacaga
     1441 tacatggtaa atcttaagga tttaaagatt cctaaccaga gagtacctga gtatacaaag
     1501 gaagagcgcc agagaatgca gagagccttc ggttacacat atgagtcact caaggattca
     1561 atccttccga tggctaaaaa tggtgttgag ggcacagctg caatgggtac tgatacaccg
     1621 cttgcagctt tatcaggtaa cagggagccg ttattcaact actttaagca gcgcttcgca
     1681 caggtaacaa acccgcctat cgattcaatc cgtgaggagg tagttacatc aaccacactc
     1741 tatatcggtg aggccggaaa cgtgcttgag gagaagccgg aaaactgccg tgtacttaag
     1801 attaacaacc caatccttac aaacaccgac cttatgaaga ttaagaattt aaaggccgat
     1861 ggctttaagg tagaggtttt acctattata tattacaaga acacaagtct tgagaaagca
     1921 gtagacagac tttatataga ggctgaccgt gcatacaggg acggagccaa cattattatc
     1981 ctctcagaca ggggtgttga tgagaaccac gttgcaattc cttcactgct tgcagttgct
     2041 gcattgcagc agtatcttgt aaagacaaaa aagagaacat cgctttcgct cattcttgag
     2101 tcaggagagc cgagagaggt tcatcatttt gctacactgc ttggctttgg tgcaagtgca
     2161 atcaaccctt atctggcaca ggatacagtt aagcagcttg ttgacgagca tatgcttgac
     2221 aaggattatt atgcagcaat cgatgattac aaccatgcaa tcatcacagg tatcgtaaaa
     2281 attgcagcaa agatgggtat ttcaaccatc cagtcatatc agggctcaaa gatttttgag
     2341 gcaatcggta ttgacaagag tgttattgat aagtatttca caaatacagt aagccgtatc
     2401 ggtggaatta cattacagga tatcgagaac gatgtgaatg agcttcactc tgcggcctac
     2461 gatccgctcg gacttgagac agatgttaca cttgacagca agggcagaca caagatgaga
     2521 agcggagctg atgaccatct gtacaatccg gctacaatcc atcttctcca gcagtcgaca
     2581 cagcgcggtg actacaatct gttcaagcag tacacagcac ttgtggacga ggaagagaaa
     2641 aatacaaata tccgtggact tatggatttc aactatccta agaagggtgt caagctcgag
     2701 gaggttgaga gcgttgactc aattgtgaca cgtttcaaga caggtgctat gtcatacgga
     2761 tctatttcca aggaagcaca tgagacactt gcaattgcca tgaaccatct gcacggaaaa
     2821 tcaaataccg gtgagggtgg agaggacaag gatcgtctca caatcggaaa agacggaaag
     2881 aaccgctgtt cagcaatcaa gcaggttgct tcaggacgtt ttggtgtcac aagcagatac
     2941 ctaacaagcg cacaggagat tcagattaag atggcacagg gtgctaagcc gggtgagggc
     3001 ggacacctac ctggaaagaa ggtttatcct tggattgcaa agacaagact ttcaacaccg
     3061 ggtgttgcac tcatctctcc accaccacac cacgatattt attctatcga ggatttggag
     3121 cagcttatat tcgacttaaa gaatgcaaac agagatgcaa gaatctcagt caagctcgtt
     3181 tctgaggccg gtgtaggaac tgttgcggca ggcgttgcaa aggccggagc acaggtagtg
     3241 cttatctcag gctacgacgg aggaacagga gccgcaccta gcagctcaat ccacaatgcg
     3301 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnc ttggacttgc agagacacac cagactcttc
     3361 ttatgaatgg tcttagaaat aaagtaagaa ttgagacaga cggtaagctc atgagtggcc
     3421 gtgatgtggc aatagcagcg tgtcttggag cagaggaatt cggctttgcg acagctccgc
     3481 ttgtaacaat gggctgcgta atgatgaggg tatgtaactt agatacatgt ccggcaggaa
     3541 ttgccacaca aaaccctgag cttcgtaagc gctttgcagg aaagcctgag tacgttgaga
     3601 acttcatgcg tttcatcgct gaagagcttc gcgagtatat ggcaaagctt ggcgtaaaga
     3661 cagtggatga gctcgttgga cgtggagatc tcttaaagaa gcgtgataac ctaagcgaga
     3721 gacagtcaaa gattgacctt gactgtatac ttaataatcc gtttgacggc aaaaagcaga
     3781 aggttatatt tgatcctaag caggtatatg actttgagct ttcaaagaca aaggatatga
     3841 ccgagatcat gacacagctt aagagtgcac ttgagagcgg acaaaagcgc gcgattgaga
     3901 ttgaggtaac aaatacaaac agatctctcg gaacaatatt tggttctgag attacaaaga
     3961 aatatgacga tacgctggat gaagacacat ttgttatcaa gtgtaacggt gcaggcggac
     4021 agagctttgg agcatttatc cctaagggac ttacacttga gcttgtcgga gattcaaatg
     4081 attacttcgg aaagggtctt tcaggtggaa agctcatcgt atatccacct gcaggagtca
     4141 agtttgagaa ggatgataat atcatcatcg gaaacgtggc actttacgga gctaccagcg
     4201 gtaaagcctt cataaacggt gttgccggtg agcgtttctg cgtaagaaat tccggtgcaa
     4261 cagcagtagt tgagggtgtc ggtgatcatg gctgtgagta catgacaggt ggccgcgtag
     4321 ttatccttgg aaagaccgga aaaaactttg cagcaggtat gagcggtgga atagcatatg
     4381 tgcttgatga ggacaatgac ctttacatga gaaccaacaa acagatggta tttactgagg
     4441 aggtctcaaa caagtatgat gtattagagc ttaaggaaat gataaaagag catgtaacac
     4501 ttacaaactc agaaaagggt aaggaaatcc ttgatcattt cagtgagtat cttcctaagt
     4561 tcaagaaggt tattccttac gattacaacc gtatgcagat ggctattgta cagatggaag
     4621 aaaagggtct taacgcagag caggcacaga ttgaggcatt ttatgccagc acaagaggct
     4681 aaggaggacg cagataatgg gaaaaccaac aggatttatg gaatatgaca gatgcgaagc
     4741 agcgtctgta gagccaaagg agcgtattaa gaactttaac gagttccatg tgttcctttc
     4801 aaaagaaaat caacagaaac aggctgcccg ttgtatggac tgcggagttc ctttctgcca
     4861 gtcgggaatg acaatagcag gcatgacatc aggctgtcct ctgcacaacc tggttccgga
     4921 gtggaacgac cttgtatata ccggcaactg ggagcaggca tataatcgtt tgcacaagac
     4981 caataatttc cctgagttta catcaagagt ctgcccggca ctgtgcgaga aggcatgtac
     5041 atgcggacac tggggcagcc cggtgtcagt cagggacaat gagcatggtg taatagagac
     5101 tgcatatgca aacggatatg cagctccaaa gccaccaaag gtcagaaccg gcaagaaggt
     5161 ggcaatcatt ggatcgggtc cttcaggact tgcggcagca gaccagctta accagagagg
     5221 ccatgaggtt acagtatatg agagagagga ccgcgtcggt ggcctgctca tgtatggtat
     5281 tccaaatatg aagcttgata agagcattat tgacagaaaa gttaatataa tgaaggaaga
     5341 gggcattcat tttgtcacag gctgtgatgt cggaaaggat ataaagccgg cagagcttta
     5401 caagaagtat gacagggtaa tcctttgctg tggtgccaaa aatccaagag acataaaggc
     5461 agacggaaga gatgcaaagg gtatttactt tgctgttgat taccttaccc agaatacaaa
     5521 gagcctttta aattcaaacc tgacagatgg caagtttgta tctgcaaagg gcaaaaatgt
     5581 agtcattatc ggtggcggtg acacaggaaa cgactgtgtc ggaactgcaa tccgtcaggg
     5641 cgccaaatca gtcacacagc ttgagatgat gccttgtccg cctacagagc gtgccgcgaa
     5701 caatccgtgg cctgagtggc caaaggtctt aaagacagat tacggccagg aggaagccat
     5761 tgcagtattc ggacatgatc cacgtatcta caagacaacc gtaaaagagt tccacaagga
     5821 caaaaacggc aacttaaagg agcttacaat tgtaagtctt gagagtaaaa aggacgagaa
     5881 gacaggcaga ttcatgatgg ttccggtaga gggatcagag aaaaagcttc cggcagagct
     5941 tgtgcttata gcagcaggct tccttggaac agaaagctat gtggcaaagg catttggtgt
     6001 agagctcaat gccagaacaa acgtaagtac acaggaggga cattacgcta caaacgtaaa
     6061 aaacgtatac gtggcaggtg ataaccaccg tggacagtcc cttgtagtat gggctatcag
     6121 agagggacgt gaggctgcaa gagaggttga tgagagtctc atgggata
//
LOCUS       DPUF01000009            3847 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_41316, whole
            genome shotgun sequence.
ACCESSION   DPUF01000009 DPUF01000000
VERSION     DPUF01000009.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_41316"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1868)
                     /locus_tag="DHV81_00240"
     CDS             complement(<1..1868)
                     /locus_tag="DHV81_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI92983.1"
                     /translation="MKSKTSFINKTMLQKNVKLYWPIWTLYTIVLLFNGPFSMWSRFK
                     SAYFIYGTNWHKYMLDIMSPAISMEADMIFIFVMALVTGMAMFSYLYNSRACNMIHAM
                     PVTRRQLFSTNVLTGLLFMWIPQIVKYIMSFVICISYGNTKVVHIGINLLATMGISFF
                     MYSLVCLCAMITGQLVSVAVMYALFNLLYGGAVIAIANVLTYVSYGLSYMEFVRKISA
                     TWFAPMLQLLNRVGFHPTMKNAGDDYYCIKYTFRGTNTIVVYVIAAAVIYFISYKIYK
                     HRDLENAGSFIAIPKLKPVFRWGLGSLGGLILSIVAASLLLGLRISIGVPAIMMLAVV
                     LGIIAFLLLEMIIRKNFKIFSKALFKEIIAFGAFVVVVFGGITVYGNVQENYIPKLAD
                     IDSACIAIDFDINLEGKDVEKILETQKILMAQKKDYFKKRYDDSGYITISYTLKNGEK
                     VNRVYHTTDDFNPHKQCKAIMAEENKPQNIINAIMQCDTTDITFIDGSAEQYDDKYVD
                     VLKESFNGKVAADIFEAVKKDVEAGVMQEYNLQRMLDGVDKDNSYMYNLMLNFTVPKG
                     NRIGKSWNVYDFTWYEELLDILGVTKEYSDFGDARSDGIETYSVNISFGENCTNLIA"
     gene            complement(1843..2748)
                     /locus_tag="DHV81_00245"
     CDS             complement(1843..2748)
                     /locus_tag="DHV81_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI92984.1"
                     /translation="MIQINNLVKKFDDFTALNGVNMNVEKGAVYGLVGPNGAGKSTLI
                     RHITGVMRQDAGEVFIDGEPVYENARIKGQMAYIPDDMFYFLQSDTINMMHYYKGMYP
                     GFDEKQFYRMQEFFPSIDVKRNIRKLSKGMQKQVAFWLALCCKPKLIVLDEPVDGLDP
                     VMRRQIWSIMLDDVAANNTTVVVSSHNLRELEDVCDHVGILNKGKIMIERSLTELQGN
                     ISKIQIACPYGMPKIPQDFKVLHMSNIGRVYTVIVRGEPEAVVKAITDGKDSPQVVDV
                     LPLTLEEIFIYEMGGEDYEVKDIIY"
     gene            complement(2732..3181)
                     /locus_tag="DHV81_00250"
     CDS             complement(2732..3181)
                     /locus_tag="DHV81_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI92985.1"
                     /translation="MQLNYRDSKPIYEQIKEGIRRMIVMNVFKQDEKLPSVRVLACKY
                     AINPNTIAKAYRELEEEGFIYTQSGKGTFVADANNARRSRAKHLKLLFDQVVTELFVL
                     DTPVEELMTRMDGAKAHLDIEDDASGFVIQQIRAEAAVTEGNDDTDK"
     gene            complement(3362..>3847)
                     /locus_tag="DHV81_00255"
     CDS             complement(3362..>3847)
                     /locus_tag="DHV81_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HCI92986.1"
                     /translation="PNVVLATFYADCVPLYFVDPVHRAIGLSHSGWRGTVNRMGKETL
                     IAMNREFGTEAHDVIAAIGPSICQDCYEISQDVAEHFVDEFATTDDPHASDILRYKGA
                     GKYQLDLWECNRRVLVEAGVQEANIAITNICTCCNPTLLFSHRASHGKRGNLGAFMFL
                     K"
BASE COUNT         1160 a          956 c          585 g         1146 t
ORIGIN      
        1 gctatcagat tagtacagtt ttcgccaaaa ctaatgttga ccgaatacgt ttcaatacca
       61 tcggaacggg catccccaaa atcagaatac tcctttgtca cacccagtat atccaacagc
      121 tcctcatacc atgtgaagtc atagacattc cagctttttc caatcctgtt gccctttggc
      181 actgtaaaat tcagcataag attgtacata tagctgttat ccttatccac tccgtcaagc
      241 attctctgca aattatattc ctgcatcaca ccggcctcaa catccttctt cacagcctca
      301 aaaatatccg ctgcaacttt tccgttaaag ctctccttca gcacatcaac atatttatca
      361 tcatactgct ctgccgagcc atcgataaag gtgatatctg tcgtatcaca ctgcattatg
      421 gcattaataa tattctgtgg tttattttcc tccgccataa tcgccttaca ctgcttatgt
      481 ggattaaaat catccgttgt atgatataca cggttgacct tttctccatt ttttaatgta
      541 tatgagattg taatataccc ggaatcatca tatctctttt taaaataatc ctttttctgc
      601 gccataagta tcttttgagt ctctaaaatc ttttcaacat cctttccctc cagattgata
      661 tcaaaatcta tggctataca ggcagaatca atatccgcaa gcttcggtat ataattttcc
      721 tgcacatttc cgtaaactgt tatgcctcca aatacaacta caacaaaagc accaaaagca
      781 atgatttcct taaatagtgc tttagaaaaa attttaaaat tcttccttat aatcatctct
      841 aaaagtaaga aggctatgat tccaagcacc actgccagca tcataattgc aggaacacca
      901 atgctgattc taagtccaag caaaagcgac gctgcaacaa tagataaaat caatcctccc
      961 aaactgccaa gcccccatct gaaaaccggc ttaagctttg gaatagctat aaaacttcct
     1021 gcattttcca aatctctatg cttataaatc ttataagaaa taaagtatat aactgcagcc
     1081 gcgatgacat agactacgat agtatttgtg cctctgaagg tatatttaat gcagtaataa
     1141 tcatcccctg cattcttcat ggtcggatga aagcctaccc gattgagaag ctgtagcatg
     1201 ggtgcaaacc acgtagcact tattttcctt acaaattcca tatatgaaag cccgtacgat
     1261 acatatgtga gcacattggc aatagcaata actgctcctc catacagcag attgaaaagc
     1321 gcatacatta ctgctacaga aacaagctgt cctgtaatca ttgcgcacag acacaccagg
     1381 ctgtacataa aaaaagatat tcccattgta gcaagcagat tgattccaat atgtaccacc
     1441 ttcgtattgc catatgatat acatataaca aacgacataa tgtacttaac tatctgcgga
     1501 atccacataa acagcaggcc tgtcaacaca ttagtcgaaa aaagctgacg tcttgtcaca
     1561 ggcatagcgt gaatcatatt acatgctctc gaattgtaca ggtaactgaa cattgccatg
     1621 cctgtaacca atgccataac aaaaataaaa atcatatctg cctccatgct gatagccggg
     1681 gacattatat caagcatata cttatgccag ttcgtgccat aaataaaata tgcactctta
     1741 aatctgctcc acatagaaaa agggccgtta aataatagca ctattgtata cagtgtccag
     1801 attggccagt aaagctttac attcttttgc agcattgttt tattaataaa tgatgtcttt
     1861 gacttcatag tcctctcctc ccatctcata gataaatatt tcctccagag taagaggcag
     1921 tacatccact acctgcggac tgtccttgcc atctgtaata gcctttacaa cagcctcagg
     1981 ctctccacgc acaataactg tgtatactct tcctatattt gacatatgaa gaactttaaa
     2041 atcctgcggt atcttaggca tgccatatgg acatgcaatc tgaatctttg aaatattacc
     2101 ctgcagctct gtaagagagc gctctatcat aatttttccc ttattgagaa tacctacatg
     2161 atcacacaca tcctccagct cgcgcaggtt atgagacgat acaaccactg tagtattatt
     2221 tgcagccaca tcatcaagca tgatagacca tatctgtctt ctcatgacag gatcaagccc
     2281 atcaacaggc tcatcgagta caataagctt tggtttacag cacagcgcaa gccagaaggc
     2341 aacctgcttc tgcatgccct tagagagctt tctgatattc cttttaacat ctatagatgg
     2401 aaaaaactcc tgcatcctgt aaaactgctt ctcatcaaag cccggataca ttcccttata
     2461 gtaatgcatc atattaatcg tatcagactg caaaaaataa aacatatcat ccggtatata
     2521 agccatctgc cctttaatcc ttgcattttc atataccggc tcaccatcaa taaacacttc
     2581 ccctgcatcc tgtctcatta cgcctgtaat atgcctgata agtgtagact ttccggctcc
     2641 gtttggacca acaagcccat agacagcacc cttttccacg ttcatattca cgccgttgag
     2701 tgctgtaaaa tcatcaaatt ttttcactaa attatttatc tgtatcatca ttaccctctg
     2761 tcaccgcagc ttctgcccgt atctgttgta ttacaaatcc ggatgcatca tcttcaatat
     2821 ctaaatgcgc cttcgcacca tccatcctgg tcatcaattc ctctaccggt gtatcaagca
     2881 caaataactc tgtcaccacc tggtcaaaaa gcagcttcaa atgttttgcc ctgctccgcc
     2941 ttgcattgtt agcatctgca acaaaggttc ctttaccact ttgagtgtaa ataaatcctt
     3001 cctcctcaag ctctctgtag gcctttgcaa tagtgttagg attgattgca tacttgcagg
     3061 caagcacccg gacagacgga agcttttcgt cctgtttgaa aacattcata acaatcattc
     3121 gtctgatgcc ttccttaatt tgttcataaa taggctttga atctcgataa tttaactgca
     3181 tatcgttctc cctggttcat atatggcagt accgctgtag cttttaatat taactcaaaa
     3241 aagtatcaaa ctgtctgacc gcatcagttc ccgcacagtg tactatccaa cgtgttacac
     3301 ctattttaac atatatagct ttaggcgttt cttattattt ccttaataga ttgtgcgaaa
     3361 attacttaag aaacataaaa gctccaagat ttcccctctt tccatgactt gctctatgcg
     3421 aaaataaaag agtgggatta caacaggtac atatattggt tatggcaata ttggcttcct
     3481 gcactcctgc ttccacaagt actctcctgt tacattccca aaggtctagc tgatatttgc
     3541 cagctccctt atagcgcaga atatcactcg catgaggatc gtccgttgtc gcaaactcat
     3601 ccacaaaatg ctctgcaaca tcctgactta tctcatagca gtcctgacat attgaaggtc
     3661 ctatcgcagc tataacatca tgtgcctcgg taccaaactc cctgttcata gcaataagtg
     3721 tctcttttcc catgcgattt acagtacctc tccagcccga atggctaagc cctattgccc
     3781 tatgtaccgg atcaacaaaa taaagcggta cacaatcagc ataaaatgtg gcaagcacaa
     3841 catttgg
//
LOCUS       DPUF01000010            8139 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_30155, whole
            genome shotgun sequence.
ACCESSION   DPUF01000010 DPUF01000000
VERSION     DPUF01000010.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_30155"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..>926
                     /locus_tag="DHV81_00260"
     CDS             <1..>926
                     /locus_tag="DHV81_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HCI92987.1"
                     /translation="VAPFKSQNMALNSYITDEGLEMGRAQVMQAEAAGIKPMVCMNPI
                     LLKPTNNTGSQVIVNGRVLKNMPAREYFAYKKTLIPDIKKAFKKLEEYADIIVIEGAG
                     SPAEINLKENDIVNMGLAHMVDAPVLLVGDIDRGGVFAQLLGTLMLLTPEEKARVCGL
                     IINKFRGDKTILDPGIQMLEERGGVPVTGVVPYMDVQLEDEDSLTERFDKKTDGLIDI
                     AVIRYPRISNFTDFNVFEQMSEVTVRYVSTVNELRHPDIVFLPGSKNTMGDLLWMRQN
                     GLEAAVKKLSCEIPVFGICGGYQMLGASIADP"
     assembly_gap    927..1094
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1095..1379
                     /locus_tag="DHV81_00265"
     CDS             <1095..1379
                     /locus_tag="DHV81_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HCI92988.1"
                     /translation="EQGACADRKNELNNVISDGRNVYGSYIHGIFDTAEAARVIVDYI
                     ADKKGIDVNDSAILSYKSFKEKQYDRLADTLREYLDMDAVYGMLREAKYD"
     gene            1372..2474
                     /locus_tag="DHV81_00270"
                     /pseudo
     CDS             1372..2474
                     /locus_tag="DHV81_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742766.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
     assembly_gap    1647..1719
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2475..3101
                     /locus_tag="DHV81_00275"
     CDS             2475..3101
                     /locus_tag="DHV81_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobinamide kinase"
                     /protein_id="HCI92989.1"
                     /translation="MITLITGGSGSGKSAYAEKYICHASNEKGYKEKYYIATMQVFDD
                     EGQRKIDRHRRLRAGKGFITIEQPRNIQNAVYKLQSESSLKTGRSALLECMSNLVANE
                     MFPPVDASGMQAAEAEKEALDDSENMKDYEIAQISRVSKKVLKEVSILSENVAELVIV
                     TNNVFEDGVSYDQSTMNYIKAMGIVNRGLAAMAERVVEVVAGIPVAVK"
     gene            3126..3890
                     /locus_tag="DHV81_00280"
     CDS             3126..3890
                     /locus_tag="DHV81_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCI92990.1"
                     /translation="MKLIRSCIVSFSMYSKIPMPQFKWNDDDMRYMLVFFPWIGAVIG
                     LLLMLWRYIYSHFGVADICYVCVGALIPIAVTGGFHIDGFMDTMDAFHSYKPREEKLT
                     ILKDSHIGAFAVIMLAAYGLLFMGAFSQIMNDKAFIVFCAGFFIARCLSGIAVVSFKS
                     AKSDGLLFMFADTAHRTIVRAALYIQLALCIAVLLIVSLPYAVAMIIAAALSFWYYYV
                     KTKKELGGITGDTAGYFVCICECAMAVALGGVSFII"
     gene            3979..4425
                     /locus_tag="DHV81_00285"
     CDS             3979..4425
                     /locus_tag="DHV81_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide kinase"
                     /protein_id="HCI92991.1"
                     /translation="MKLVIGGVAQGKLDYVLENMIEESEKYDVYDCFFLKDDACNDKA
                     SNMEWPWDFAVDDESILIIDKFHYFIRAVLEKNLPLQEYILKFMQFAEENKDTIVIAD
                     EIGNGIVPLDAFEREYREQTGRAEILLAKKADEVVRVICGIGQKIK"
     gene            4398..5072
                     /locus_tag="DHV81_00290"
     CDS             4398..5072
                     /locus_tag="DHV81_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCI92992.1"
                     /translation="MWNRSENKINCIFIRHGCTVSNREHRYLGRTDEPLDEAGVFELE
                     NALNNGVYKALEENSLKEQIIITSPMRRCKQTAEIILPVAQHHKIHTVKEFAEMDFGE
                     WELKSYKELSTDIRFRDSYQAWIDSGGTLAFPNGESRKEFCKRSICGFERALTQALEL
                     KRAMPDKEIQIVFFVHGGTIMAIMSHYCDMDYYDYQVKNGCGYSCEVAVEGNEIVFNE
                     IVPISL"
     gene            5095..6087
                     /gene="cobD"
                     /locus_tag="DHV81_00295"
     CDS             5095..6087
                     /gene="cobD"
                     /locus_tag="DHV81_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobD"
                     /protein_id="HCI92993.1"
                     /translation="MCYHIFAFIAGFVLDLLIGDPHFIPHPVRLIGSLISFLDKRLNC
                     DAKYNISEKKLNLIKYKRGMLLVFAVIFATFTMSVIIIVAAYSINLYAGVIAEAVMTW
                     QILATKCLRVESMRVYDALRTDGVDAGRRAVSMIVGRDTSVLDAAGVTRAAVETIAEN
                     TSDGVIAPMLYTAIGGPVLGFVYKAVNTMDSMIGYKNDKYMYFGRFAARLDDVVNFIP
                     ARISAYMMIVAAFIGGRQFDGQNAYRIFKRDRFNHASPNSAQTESVCAGALRVRLAGD
                     AVYFGKLVKKKYIGDRLREIEYEDIKRANRLMYITAFLCELLSVAVMSLVLILL"
     gene            6127..7188
                     /locus_tag="DHV81_00300"
     CDS             6127..7188
                     /locus_tag="DHV81_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thr-phospho decarboxylase"
                     /protein_id="HCI92994.1"
                     /translation="MKSIHGGDIYNNKVNMDFSVNINPLGIPHSVKEAMSDALSYADR
                     YPDMACSGLKKAISEYFTQQDCSIQSEDIIPGNGASELFMAIAHAIKPQKAVLLAPGF
                     FGYEHVLGAVGCDIRYCLLDEQSDFSPVARLDALMDMLTEDTDIFFIANPNNPTGYLA
                     DSTFMKQIIGHCKEKHIYVVADECFMGFCEKKYSVLSLLCDYDNLIVVRAFTKLFAIP
                     GVRLGYMLSKNEAVRQKVQRNLPEWNVSVLAQMAGEACIRESEYIKETALYVSRQRRL
                     LSDGLKKLGFKVYKSDADFILFYSKLSLYDILLNKGILIRDCSNYVGLSQGYFRVAVK
                     TFDENIRLLKVIGECIEKN"
     gene            7202..7816
                     /gene="cbiC"
                     /locus_tag="DHV81_00305"
     CDS             7202..7816
                     /gene="cbiC"
                     /locus_tag="DHV81_00305"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103381.1"
                     /note="catalyzes the interconversion of precorrin-8X and
                     cobyrinic acid; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HCI92995.1"
                     /translation="MEIEKRSFEIISHELSEMQITLPADQEMITKRVIHTSADFDYIQ
                     TLKYSKDAVAIAKRLISEGADIVTDTNMALSGINKKYLAKFGGQAHCFMADDEVALIA
                     KEKKTTRAAVSMEFASRIDKPVIFAIGNAPTALIELYDMIEKGIYKPAFVIGVPVGFV
                     NVEAAKELIMETGVPYIVNVGRKGGSNIAAAICNALIYSLVDRG"
     gene            7839..>8139
                     /locus_tag="DHV81_00310"
     CDS             7839..>8139
                     /locus_tag="DHV81_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A synthase"
                     /protein_id="HCI92996.1"
                     /translation="MRYGFTTGSCAAAASKAAAYMLLTGKAKNKITIQTPKGIVYTAD
                     ITDIKRSENEVSCAVIKDGGDDPDVTTGAYIYASVRILRSDTCALCKKKQDLAIIN"
BASE COUNT         2398 a         1280 c         2090 g         2130 t
ORIGIN      
        1 gtggcaccgt ttaaatcaca gaacatggcg ctcaactctt atataacaga cgaaggactt
       61 gagatgggac gggcccaggt gatgcaggct gaggcagcag gcattaagcc tatggtgtgc
      121 atgaatccta ttcttttaaa gcctacaaat aatacaggct cacaggtgat tgtaaacgga
      181 agggtgctta agaacatgcc ggcgagagag tattttgcat acaagaaaac tctcataccg
      241 gatataaaaa aggcatttaa aaagcttgag gagtatgctg atatcatagt gattgagggc
      301 gctggaagcc cggcggagat taatctgaag gaaaatgata ttgtgaacat ggggcttgcg
      361 catatggtgg atgcaccggt gctgctcgtg ggtgatatcg acagaggtgg tgtttttgct
      421 cagcttctcg gcacgcttat gcttttaact ccagaggaaa aggcgcgtgt ctgtgggctt
      481 ataatcaata aatttcgtgg agacaagacg atccttgacc cggggataca gatgcttgag
      541 gagagaggcg gggtgcctgt taccggagtg gtgccatata tggatgtaca gcttgaggat
      601 gaggacagcc tgacagagcg ttttgacaaa aagacagatg ggcttattga tatagcggtc
      661 atcagatatc cgagaatatc aaatttcacg gatttcaatg tctttgagca gatgtccgag
      721 gtaacagtgc ggtatgtgtc aacagtaaat gagctgcgcc atccggatat agtatttctg
      781 ccgggaagca agaatacgat gggagatctt ttgtggatga gacaaaacgg actcgaggcc
      841 gctgtaaaaa agctctcctg tgagatacct gtttttggaa tatgcggagg ctatcagatg
      901 cttggggctt caatagccga ccccgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnngagcag ggggcttgtg ctgacaggaa aaatgagtta aataatgtca
     1141 tatctgatgg caggaatgtg tatggctcgt atatccacgg catttttgat actgcagagg
     1201 ccgccagagt gatagtggat tatattgctg ataaaaaggg cattgatgtg aatgacagtg
     1261 ctattttaag ctataagagc tttaaggaaa aacagtatga caggcttgcg gatacactga
     1321 gagagtatct ggatatggat gcagtgtacg gcatgcttcg tgaggcaaaa tatgattgaa
     1381 aacaatatta tttctgacca ttttacagtt gaagaattaa aaaaattaaa aataacgcca
     1441 cctgatgtaa aaatgtacaa aaaaatccgt tcaaactggg attcagtggc aaagccgctc
     1501 gacggcatgg gtgattttga gcttttaacg gccaaaatcg gcgctgtttt gggagatgaa
     1561 aatatagata tttcaaaaaa ggcggtcata atgatgtgtg cggataatgg tgttgtcgct
     1621 gagggcatca gccagtccgg acaggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gactgcaggg gctgatacaa
     1741 ttcctgtgga tataggaata aacagtgacg agagcgttaa agggctgctt caaagaaagg
     1801 tgcgcatggg cactagaaac tttgcaaaag agcctgccat gacaagggct gagacacttg
     1861 aagcgattgc agcaggtata gaaatagtgc gtgggtgtaa ggcagacggc tgcagaatta
     1921 ttgcaaccgg tgagatgggt ataggaaata ccaccacaag cgcggcaatg gcagccgcca
     1981 tgctaaggtg cgatgtggcc acagtgacag gaagaggagc aggcctaaac gacagtgggc
     2041 ttgagaaaaa aatccgtgta attgagagcg caatagaaaa gtatgattta tataataagg
     2101 acacctttga gatacttatg acagtcggag gactcgatat cgcaggactt gtcggagtgt
     2161 gtataggcgg ggcattatac catattcctg ttgtgctcga cggagtgata agcatggttt
     2221 cagcgttggc agcaggaagg cttctttccg gagttactga ttttatcatt ccatcacatg
     2281 agggcaggga gcctgccgtg gcgcttttgt gtgaggcact ttccgtaaag ccggtcataa
     2341 gagcatccct tgcgcttgga gagggaacag gtgccgtgat gatgtttgcg cttttggata
     2401 tggcacttag cgtatacaaa ggacggacaa ccttcgacga tatctcggta tcacagtatg
     2461 agaggtacgt atagatgatt acacttataa caggcggcag cggcagcggc aaatcagcat
     2521 atgcagaaaa gtatatttgt cacgcgtcaa atgaaaaggg atacaaagaa aaatattata
     2581 ttgccaccat gcaggtattt gatgatgagg gacagcggaa gatagacagg cacagaagac
     2641 ttcgtgccgg aaaaggcttt attaccatcg agcagcctcg taatatccaa aatgcggttt
     2701 ataagctgca atcagaaagc agtttaaaaa ccgggcggtc agcactgctt gagtgtatgt
     2761 caaaccttgt ggcaaatgag atgtttccac ctgtagatgc ttccggtatg caggctgccg
     2821 aagctgaaaa agaggctttg gatgactctg aaaatatgaa ggattatgag attgctcaaa
     2881 tcagccgtgt gagtaaaaag gtgttaaaag aggtaagtat cttaagtgaa aatgttgcgg
     2941 agcttgtaat tgtgacaaac aatgtatttg aagacggagt cagctatgat caatcaacga
     3001 tgaattacat aaaggcaatg ggaattgtaa acagagggct tgctgcgatg gcagagcgtg
     3061 tcgttgaggt ggttgcaggc attccggttg cggttaagtg aagcagtgtg gaggttaagt
     3121 gtattatgaa gttgataagg tcgtgcatag tatctttttc catgtattca aagataccaa
     3181 tgccacaatt caaatggaat gatgatgaca tgaggtacat gctggtattc tttccgtgga
     3241 taggagctgt aatcggactg cttcttatgc tgtggagata tatatactct cattttggag
     3301 tggcagatat ctgttatgta tgcgtaggtg cactgattcc gatagcagtt acgggaggct
     3361 ttcacataga tggttttatg gatacaatgg atgcatttca ttcatacaaa cctcgggagg
     3421 aaaagcttac aatacttaag gattcacata taggagcgtt tgccgttata atgcttgcag
     3481 cctacggatt attattcatg ggagcgtttt ctcagataat gaatgataaa gcatttattg
     3541 ttttttgtgc aggctttttc atcgcaagat gcttaagcgg aatagctgtt gtgtcattta
     3601 aatcggcaaa aagcgatgga ctgttattta tgtttgcaga cactgcacac agaacgatcg
     3661 tgagggcagc actatacata cagcttgcac tgtgtatagc tgttttactc attgtgtcac
     3721 tgccttatgc ggtggccatg atcattgcgg cagcacttag cttttggtat tattatgtga
     3781 aaaccaaaaa ggagcttggc ggcatcacag gcgatacggc agggtatttt gtgtgtatat
     3841 gtgagtgcgc catggcagtg gctctgggtg gtgtctcttt tattatttag tatatttgtt
     3901 ttgaatatag gaatggtacg gataagaatt ttgataaaag ttgtactatg aaccttaaac
     3961 tttggaattg gagaaaatat gaagcttgtg ataggcggag tggcacaggg gaagcttgat
     4021 tatgtgcttg aaaatatgat agaagaatca gaaaaatatg atgtgtacga ctgttttttc
     4081 ctaaaggatg atgcctgcaa tgataaggca tcaaacatgg aatggccatg ggattttgcc
     4141 gtcgatgatg agagtatact cataatagat aaattccatt attttatcag ggctgtactt
     4201 gagaaaaatc ttccactgca ggaatatatt ttgaaattta tgcagtttgc agaggaaaat
     4261 aaggatacaa tagtcattgc tgatgagatt ggaaacggca tagtgcctct tgatgcgttt
     4321 gagagagagt acagggagca gacaggcaga gcagagatac tgcttgcaaa aaaagcagac
     4381 gaggtagttc gcgtaatatg tggaatcggt cagaaaataa aataaactgt atttttataa
     4441 gacacggctg cactgtctca aacagagagc acagatatct gggaaggaca gatgagccac
     4501 tcgatgaagc cggagttttt gaactagaaa atgcgctaaa taatggtgta tacaaggctt
     4561 tggaggaaaa tagcttaaag gagcagataa ttattaccag tccaatgagg cgctgtaaac
     4621 agacggcaga gattattttg ccggtcgcac agcaccataa aattcatacc gtaaaagagt
     4681 ttgcagagat ggatttcggt gaatgggagc taaaaagcta taaggaactt agcacagata
     4741 tccgtttcag ggactcatat caggcgtgga ttgacagtgg tggaacactt gcctttccaa
     4801 acggagagag cagaaaagag ttttgcaaaa ggagtatatg tggctttgag agggcactca
     4861 cacaggcatt agagcttaag agggctatgc ctgataaaga gatacaaata gtattttttg
     4921 tgcatggagg gactatcatg gcgataatga gccattactg tgatatggat tactatgatt
     4981 accaggtgaa aaacggctgt ggctactcgt gtgaggtggc ggttgagggc aatgagattg
     5041 tgtttaatga aatagtgccc atatcgctat gatttattta agaaggacta acatatgtgt
     5101 tatcatattt ttgcatttat agcaggcttt gtgcttgatt tattaatagg agatccgcat
     5161 tttataccgc atccggtcag gcttatcggc tcactcattt cttttctcga taagaggctt
     5221 aattgtgatg ctaaatataa tatctctgaa aaaaaattaa atctaataaa atataaaaga
     5281 ggcatgcttc ttgtttttgc tgtaattttt gcaactttta caatgtcggt aattattatt
     5341 gtggcagcat acagcataaa tctatatgcc ggggtaatag cagaggctgt catgacgtgg
     5401 cagattcttg ccacaaagtg tcttcgtgtg gagagcatga gggtgtacga tgcgctaagg
     5461 acagatggag ttgatgcagg aagaagggct gtttccatga ttgttggcag agatacatct
     5521 gtgcttgatg cggcaggtgt cacaagggcg gcagttgaga cgatagctga aaatacatct
     5581 gacggagtga ttgcacctat gctttacacg gcaatcggtg ggcctgtgct tggctttgta
     5641 tacaaggctg tgaatacgat ggattcgatg attggataca aaaatgataa atatatgtat
     5701 tttggcagat ttgctgcaag gcttgatgat gtggtcaatt tcataccggc aagaatcagt
     5761 gcatatatga tgattgtggc agcatttatt ggtggcaggc agtttgatgg gcaaaatgca
     5821 tatcgcattt ttaaaagaga tcgtttcaat cacgcaagcc caaactcagc ccagacagag
     5881 tccgtgtgtg ccggggcttt aagggtgcgg ctggcaggtg atgcagtata ttttggaaag
     5941 cttgtgaaaa aaaaatatat aggagacagg ctgcgagaga tagagtacga ggatataaag
     6001 cgtgcaaaca ggctgatgta tattactgcg tttttgtgtg agcttttatc tgtggcggtt
     6061 atgagtcttg ttttaatatt gctttgaata tggaagaatt gatgtaagaa ttgatggagg
     6121 acttgaatga aaagcatcca tggcggtgac atatacaaca ataaagtaaa tatggatttt
     6181 tcggtaaata tcaatccgct tggcataccg cattctgtta aagaggctat gagtgatgca
     6241 ctttcgtatg cagacagata tcccgacatg gcatgcagtg gccttaaaaa agcaatatca
     6301 gagtatttca cacagcagga ctgcagcata caaagcgagg atataattcc cggaaacgga
     6361 gcctcagagc tttttatggc gatagctcat gccataaagc cccaaaaagc agtgctttta
     6421 gctccgggat ttttcggcta tgagcacgta cttggggcag taggctgtga tatcagatat
     6481 tgtctgcttg atgaacagag tgatttttct cctgttgcac ggttagatgc tttaatggat
     6541 atgcttacag aagatacaga tatatttttt attgcaaatc caaacaatcc gacaggatat
     6601 cttgcagaca gcactttcat gaagcaaata atagggcatt gcaaagaaaa gcatatttat
     6661 gtggtggcag atgaatgctt catgggcttt tgtgaaaaaa aatattctgt tttgtcactt
     6721 ttatgtgact atgacaatct gattgttgta agagccttta ccaagctttt tgctattccg
     6781 ggagtaaggc ttggatatat gctcagtaaa aatgaggcag taagacaaaa ggttcaaaga
     6841 aatctgccgg agtggaacgt gtctgtcctg gcacagatgg caggagaggc atgcattagg
     6901 gaatcagagt atattaaaga aacggcattg tatgtgagca gacaaagaag gctgctgtca
     6961 gatggattaa aaaagcttgg ttttaaggta tataaaagtg atgctgattt catactgttt
     7021 tacagcaagc tttcattata tgatatactg cttaataagg gaatactgat acgggactgc
     7081 tccaactatg tggggctttc acaagggtat tttcgtgtgg cggtaaagac atttgatgaa
     7141 aatataagat tattgaaagt gataggtgag tgtattgaaa aaaattaaac agttagagcc
     7201 gatggagatt gaaaagagga gctttgagat aatttcacat gagctgtctg aaatgcagat
     7261 tacgctgcct gctgatcagg agatgataac aaaaagagtc atacacacga gtgctgattt
     7321 tgattatata cagacgctta aatactctaa ggacgccgtt gctatcgcaa aaaggctgat
     7381 atcagagggc gctgatatag tgaccgatac caacatggct ctttcaggca taaataaaaa
     7441 gtatctggca aaattcgggg gacaggcgca ctgctttatg gctgatgatg aggtcgctct
     7501 tattgcaaag gagaaaaaga caacgcgcgc ggcagtaagc atggagtttg catcaaggat
     7561 agataagcct gtgatttttg cgataggaaa tgcaccgaca gcgcttatag agctgtacga
     7621 tatgatagaa aaaggaatat ataagcctgc atttgtaatc ggtgtgccgg tgggctttgt
     7681 caatgtggag gcagccaagg agcttattat ggagaccgga gtgccataca tagtaaatgt
     7741 cggacgaaag ggtggcagca atattgcggc tgctatatgc aatgcgctga tttacagtct
     7801 ggtggataga ggctgatgca tgaggaaatt tagtgattat gagatacgga tttaccacag
     7861 gaagctgtgc tgcggcggca tcaaaggcgg cggcatatat gcttttaacc ggaaaggcta
     7921 aaaacaaaat aacgattcag acacctaagg gaatagttta cacggctgat atcaccgaca
     7981 taaaaagaag tgaaaatgag gtaagctgcg ccgtgataaa ggatggaggg gatgacccgg
     8041 atgtgaccac aggagcctat atatatgctt ctgtacggat tttgcgttcc gatacctgtg
     8101 ctttatgcaa aaaaaagcag gatttggcta tcataaata
//
LOCUS       DPUF01000011            8946 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_29177, whole
            genome shotgun sequence.
ACCESSION   DPUF01000011 DPUF01000000
VERSION     DPUF01000011.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_29177"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="DHV81_00315"
     CDS             <1..306
                     /locus_tag="DHV81_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HCI92997.1"
                     /translation="GINLTSLRVDGGASANNLLMQMQADISNAPVNRPVCVETTAMGA
                     AYLAGLAVGYWDSMDDIKRNWAIDRVFEPEIEADLREKKLKMWKKAVACAFNWAKDD"
     gene            346..1782
                     /locus_tag="DHV81_00320"
     CDS             346..1782
                     /locus_tag="DHV81_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HCI92998.1"
                     /translation="MYDVVIIGAGVSGSACARELSKYNLSICVVEKEEDVCCGTSKAN
                     SAIVHSGIDCKTGTLMAQMNIRGNEMMDELSKQLDFEFRRNGSLILCFSEDDMPRLKE
                     LYNQGIANGVPGLEILDSKKAHEMEPNLSDEVVAAIYCPTGGIVCPFGMNIAFAENAA
                     ANGVEFLFNTEVKEIQKEQAGYILITQNGEIHARCVVNAAGVYADVFHNMVSENKIHI
                     TPRRGEYELLDRAAGGIVDKTIFQLPGKYGKGVLVTPTVHGNILIGPTADDHDDKDGT
                     NTTRAGLAHVTTSGTRSVPSLPMRQVITSFAGNRAHEDGHEFIIEELKDAPFFVDCAG
                     IESPGLSSAPAIGERVAAIVERLFKPSKNADFIETRKGILNPKKLSEDEYKELIKEKP
                     EYGNIICRCEMITEGEIMDAVNRPLGAKSLDGVKRRTRAGMGRCQAGFCSPRTMEIIA
                     RERGISQLDITKSGGKSKIVVGMSKNRG"
     gene            1857..3152
                     /locus_tag="DHV81_00325"
     CDS             1857..3152
                     /locus_tag="DHV81_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCI92999.1"
                     /translation="MEFYDIVIVGGGPAGLAAAASAKKNKIDKILILERDCELGGILN
                     QCIHNGFGLHTFKEELTGPEYAGRFIKEVKELGIEYRLNTMVMDISTGDESENGSNDK
                     IVTAMNRTDGLFHIAAKTVILAMGCRERARGALNIPGYRPAGIFSAGTAQRLVNIEGY
                     MPGRKVVILGSGDIGLIMARRMTLEGAKVQVVAELMPYSGGLKRNIVQCLDDFNIPLK
                     LSHTVVDIKGRERVEGITLAQVDEHNKPIEGTEEFYECDTLLLSCGLIPENEISKTAG
                     VELNPVTSGPVVNESLETNVPGVFACGNVLHVHDLVDYVSEEATAAGRHAAEYVKNGG
                     DKSDDGKEISITATGGVRYTVPSTINTAQMDDTATVRFRVGAVYKNCYIAVYLDDKQL
                     QHRKHPVMAPGEMEQVVLQKKQLIETENPRTITIKIEEA"
     gene            3157..3516
                     /locus_tag="DHV81_00330"
     CDS             3157..3516
                     /locus_tag="DHV81_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HCI93000.1"
                     /translation="MEERNLTCIGCPLGCALKVTIDGDNVTVTGNTCKRGADYGANEV
                     THPTRIVTSTVCVKNGTIPMVSVKTAQDIPKEKIMDVMDCINRITVEAPVHIGDVLLS
                     DAAATGVDIVATKNVEA"
     gene            3534..4772
                     /locus_tag="DHV81_00335"
     CDS             3534..4772
                     /locus_tag="DHV81_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HCI93001.1"
                     /translation="MVKTGISLDSVKNNNKSQILKILQKNGAMSRKNIANQIGLTPAA
                     VTMLCNDMMEAHMILEKGEMKEQKRAGRKKVLVDINYDFKYIVSVNIEAVDTSITVTN
                     IRGNVLVETKVHTRTDIEPQLFLEELAKEIKLQLWEKELKLSDILGIGICVPGIVDRN
                     NGISIHAYGIWNNQVKIREILEKQIQCPVILENNVKAFAEAEMLYGVGKYGNNIVFIK
                     WGPGVGSAIVVDNKLYEGNQHNAAEIGHYIIEPDGLKCRCGRHGCLETRVSMFALCDR
                     IKEIYSKENTPVLYEETAGDKNLITRELLTSWVENEGNGYITRMDKTISEILVGAIER
                     MARVAVNVLTILAPDCTIVFGSMFENTSIYKLFIQYCTKYDENYTDKLISRSHLSDKM
                     AYIGGTALIASTYFFESKNL"
     gene            4850..5116
                     /locus_tag="DHV81_00340"
     CDS             4850..5116
                     /locus_tag="DHV81_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCI93002.1"
                     /translation="MSQSVNVNFKLDADVKKSMEQVCSELGLSMSAAFTVFAKKVGRE
                     KRIPFEVSVDPFYSDSNIRYLENIVRDIKDGKAHFAEHDLIEED"
     gene            5116..5376
                     /locus_tag="DHV81_00345"
     CDS             5116..5376
                     /locus_tag="DHV81_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCI93003.1"
                     /translation="MRLLWEDRAWSDYLYWQTQDKKTLKRINGLIKDIQRSTFEGIGK
                     PEPLKENLSGMWSRRIDDTNRIVYYEKDEIIYIVSCKGHYDD"
     gene            6198..>6571
                     /locus_tag="DHV81_00350"
     CDS             6198..>6571
                     /locus_tag="DHV81_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCI93004.1"
                     /translation="MSKELNKTYDPKDIEDRLYKKWEDNGYFHAEVDRSKKPFTIVMP
                     PPNITGQLHMGHALDNTMQDILIRYKRMQGYNALWQPGTDHASIATEVKVIQALKEQG
                     INKADLTREEFLEKCWDWRKEYG"
     assembly_gap    6572..6679
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6680..>7611
                     /locus_tag="DHV81_00355"
     CDS             <6680..>7611
                     /locus_tag="DHV81_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCI93005.1"
                     /translation="VQEVFTKLYKKGWIYKGSRIVNWCPVCKTSISDAEVEHEEQDGF
                     FWHINYPVVGEEGRFVEIATTRPETLFGDTAVAVNPDDERYQDIIGKTLKLPCTDREI
                     PVIADSYVDKEFGTGCVKITPAHDPNDFEVGKRHSLEEIVVINDDATMNEKAGKYAGM
                     DRYECRKALVEDLKEQGLLVKVVPHSHNVGVHDRCHTTVEPMIKQQWFVKMDEMIKPA
                     VEGVKNGEIKLLPSRMDKTYFNWTDNIRDWCISRQLWWGHRIPAWYCDDCGEMVVSIE
                     KPGKCPKCGKEHWTQDPDTLDTWFSSALWPFSTLG"
     assembly_gap    7612..7737
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7738..8841
                     /locus_tag="DHV81_00360"
     CDS             <7738..8841
                     /locus_tag="DHV81_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCI93006.1"
                     /translation="HGLVRDSQGRKMSKSLGNGIDPLEVIDKYGADALRLTLITGNAP
                     GNDMRFYWERVEASRNFANKVWNASRFIMMNLEGVELREPKLDELHAADKWILSRVNT
                     LAKDATENMDKFELGIAVQKVYDFIWDEFCDWYIEIAKVRTYKKDENPESANAALWTL
                     KTVLVNALKLLHPYMPFITEEIFCTLQSDEETIMLSKWPEYKEEWNFPAEEAAIEHCK
                     DLVKGIRNVRTQMDVPPSRKAKLFITSDDEAVRKVFEDNKEVYVNLAFTSEIAVQQGK
                     SGIGDDAVSVVIPDAVAYLPLEDLVDFEKEKERLNKEKDKLTKELARSRGMLSNEKFL
                     NNAKPEKVQEEKDKLAKYEQMMAQVEERLAQFK"
BASE COUNT         2812 a         1592 c         2257 g         2051 t
ORIGIN      
        1 ggcatcaatc tcacctcgct tagggtggac ggcggcgcca gtgccaacaa tctgctcatg
       61 cagatgcagg ctgatatcag caatgcaccg gtcaacaggc ctgtgtgcgt ggagactaca
      121 gccatgggtg cagcgtacct cgcaggactt gcagtgggct attgggacag catggatgat
      181 ataaagagaa actgggctat tgaccgtgtg tttgagccgg agatagaggc tgatttgaga
      241 gaaaagaagc ttaaaatgtg gaaaaaggct gtcgcctgcg catttaactg ggcgaaggat
      301 gactgaaagt aaaaatatat atggaaattg gtaggagaaa ataacatgta tgatgtagta
      361 attataggag cgggagtatc gggaagcgcc tgtgcaagag agctatcgaa atataacctg
      421 tccatctgcg tggtggaaaa ggaggaggac gtgtgctgtg gcacttcaaa ggcaaacagt
      481 gctattgtcc attccggaat tgactgtaag acaggtacac ttatggcaca gatgaatatc
      541 cgtggaaatg agatgatgga cgagctttcg aagcagcttg attttgagtt cagaagaaac
      601 ggctcgctga ttttgtgctt tagcgaggac gatatgccaa ggcttaagga gctttacaat
      661 cagggaatcg caaatggcgt gccgggactt gaaattcttg attcaaagaa agcacatgag
      721 atggagccga atctgtctga tgaggtggtg gcagctattt actgcccgac aggaggcatt
      781 gtatgtccgt ttggcatgaa tattgcattt gctgagaatg ctgcggcaaa cggtgtggag
      841 tttttattta acaccgaggt gaaggagata caaaaggagc aggcaggcta tattctcatt
      901 actcaaaatg gagaaattca tgcacgctgc gttgtaaatg cagccggtgt atatgctgat
      961 gtatttcaca acatggtgag tgaaaacaaa atccatatca caccaaggcg cggagagtac
     1021 gagctattag acagggcagc tggtggtatc gttgacaaga caatcttcca gcttccggga
     1081 aaatacggca agggcgtgct tgtaaccccg acagtacacg gaaatatcct aatcggacct
     1141 acagcagatg atcatgatga caaggatggt acaaatacaa caagagcggg cttagcgcat
     1201 gttacgacat ccggcacacg ttcggtgccg tcacttccta tgcgtcaggt catcacatcg
     1261 tttgcaggaa accgtgcaca tgaggacgga catgagttta tcatcgagga gcttaaagat
     1321 gcaccatttt ttgtggactg tgcaggtata gagtcaccgg gcttatcaag tgcaccggct
     1381 atcggtgaga gagtggcagc aatagttgag aggcttttta agccttctaa aaatgcggac
     1441 tttatcgaga caagaaaagg tattttaaat ccaaagaagc tttcagagga tgagtataaa
     1501 gagctgataa aagaaaagcc tgagtatggt aacataatct gccgctgtga gatgattacg
     1561 gagggcgaga taatggatgc ggtaaacaga ccgttaggcg caaagtcgct tgatggtgtg
     1621 aagagacgca ccagagccgg aatgggcagg tgccaggcag gcttctgttc tccgagaact
     1681 atggagatta tcgccaggga gagaggcatt tcgcaacttg acatcactaa aagcggtgga
     1741 aaatcaaaga tagttgttgg aatgagcaag aatcgcggat agtctgtctt acttcaaaga
     1801 gacaggtaac gaaagattga tattgggtta atatcagatt aacatagggg gacaatatgg
     1861 aattttacga tattgtgata gtaggaggcg gcccggcagg tcttgcggca gcagcttccg
     1921 ctaagaaaaa taaaatagat aaaatactta tactggaaag agactgcgag cttggcggca
     1981 tattaaacca gtgtatacac aatggctttg gcctgcacac ctttaaggag gaactcacag
     2041 gccctgaata tgccggcaga tttatcaaag aggtcaaaga gcttggcata gagtacagac
     2101 tgaatacgat ggtcatggat atctcaaccg gtgacgagtc cgaaaatggg tcaaacgata
     2161 aaatagtcac ggctatgaac cgtacagacg gacttttcca tatcgctgca aagacagtaa
     2221 tacttgcgat gggctgcaga gagagagcaa gaggtgccct aaacatacca ggctacagac
     2281 cggcaggaat tttctcggcg ggtacagctc agagactggt gaatatagag ggatacatgc
     2341 cgggacgaaa ggtagtaata cttggctcag gagatatcgg tcttatcatg gcaagaagaa
     2401 tgaccttgga gggtgccaag gtgcaggtgg ttgcagaact catgccgtat tcaggaggct
     2461 tgaagcgtaa tattgtgcag tgccttgatg attttaatat cccgcttaag cttagccaca
     2521 cagtggttga tataaagggc agggagcgtg tggagggcat cactctcgct caggttgacg
     2581 agcataacaa gcctattgag ggaacagagg agttttacga gtgcgataca cttttacttt
     2641 catgcggact cattccggaa aatgagattt caaaaacagc aggtgtagag ctcaatcctg
     2701 taacaagcgg acctgttgta aacgaaagcc ttgagaccaa tgtgccgggt gtatttgcct
     2761 gtggaaatgt gcttcatgtg cacgacctcg tggattatgt gtcagaggaa gcaacagcag
     2821 caggccgcca tgccgcagag tatgtgaaaa acggcggtga taagagtgat gatggcaaag
     2881 aaatatcgat aactgctaca ggcggagtca gatatactgt cccaagcact ataaatactg
     2941 cacagatgga tgatactgca acggttagat ttcgagttgg agctgtgtat aagaattgtt
     3001 atattgcggt atatctggat gataaacaat tacagcaccg caagcaccct gtaatggcac
     3061 cgggtgagat ggagcaggtg gtgctgcaga agaagcagct catcgaaacg gaaaatcccc
     3121 gcacaattac gataaagatt gaggaggcat agtatcatgg aagaaagaaa tttgacatgt
     3181 ataggctgcc ctctgggctg tgcccttaag gtgacaatag atggagataa tgtcacagta
     3241 accggaaata cctgtaagcg cggagcagac tacggagcaa atgaagtgac acatccgacg
     3301 cgaatcgtga caagcacagt ctgtgtgaaa aacggcacaa tacctatggt atccgtaaag
     3361 acggcacagg atattccaaa ggaaaagata atggatgtga tggactgtat caaccggatc
     3421 acggtagagg caccggtgca tataggtgat gtactgctaa gtgatgctgc tgcaaccggt
     3481 gtggatatag ttgcaacaaa gaatgttgaa gcataaataa ggagcatgaa ttaatggtta
     3541 aaaccggaat ttcccttgat agtgtaaaaa acaataataa atctcagata cttaagatac
     3601 tgcagaaaaa cggcgcaatg tcaaggaaga atattgccaa tcagatagga ctcacaccgg
     3661 cggctgtgac catgctgtgc aatgatatga tggaagctca catgatactt gaaaagggtg
     3721 agatgaagga gcagaagaga gcgggccgta agaaggtgct tgtggatatc aattatgatt
     3781 ttaagtatat agtatctgta aatattgagg ctgtagacac gagcattaca gttaccaata
     3841 tcaggggaaa tgtgcttgtt gaaactaagg tacatacccg gacggacatt gaaccacagc
     3901 tgtttttaga agaactggca aaggaaataa agcttcagct ttgggaaaag gaacttaagc
     3961 tgtcagatat tcttggaatc ggaatatgtg ttccgggaat agttgacagg aataatggca
     4021 tcagtataca cgcttatggc atatggaaca atcaagtaaa aataagagaa atacttgaaa
     4081 aacagattca gtgtccggta atacttgaaa acaacgtcaa agcatttgcg gaagccgaaa
     4141 tgctttatgg tgtcggaaaa tatggaaaca atattgtttt tattaagtgg ggcccgggtg
     4201 tcggctctgc gatagttgtg gacaataagc tatatgaggg caatcagcac aacgcagcag
     4261 aaataggaca ctacatcata gagcctgatg gacttaagtg cagatgtggc agacatggct
     4321 gtcttgaaac aagagtatcc atgtttgccc tgtgtgaccg cataaaggag atatattcca
     4381 aggaaaatac ccctgtgcta tacgaagaaa cagccggtga taaaaatctt ataacaagag
     4441 agctcttgac ttcgtgggta gaaaatgagg gcaacggcta cataacaagg atggataaga
     4501 caatttctga gatacttgtt ggagcaatag aacgaatggc acgtgtcgct gtaaatgtgt
     4561 tgactattct ggctccggat tgcacgatag tgtttggttc aatgtttgaa aatacaagta
     4621 tatataagct ttttatacag tactgcacaa aatatgatga aaactatact gacaagctta
     4681 tcagccgttc acacttgagc gataagatgg catatatagg cggaacagca cttatagcga
     4741 gcacttattt ctttgaaagt aaaaatttat aataaagaaa aaatgaagaa atcttattga
     4801 tattttcttt attgttgcat atactataag caagaaagga gtgttgtata tgtcacagtc
     4861 agttaatgtt aattttaaat tggatgcaga tgtaaaaaaa agcatggaac aggtgtgttc
     4921 ggaattaggt ctttctatga gtgcagcgtt caccgttttt gcaaaaaaag taggcagaga
     4981 aaagcgtatt ccttttgagg tatcggtaga tccgttttat tctgatagca atatccgtta
     5041 tttggaaaat atcgtgcgcg atattaagga tggcaaagct cacttcgcag agcatgattt
     5101 gatagaggag gactaatgag gttactatgg gaagataggg catggagcga ttatttatac
     5161 tggcagacgc aggataagaa aacactgaaa agaattaatg gacttatcaa ggatattcag
     5221 cgaagtacgt ttgagggtat aggtaaaccg gaaccattaa aggaaaatct tagtggtatg
     5281 tggagcagac gcattgatga cactaatcga attgtttatt atgaaaaaga tgagataatt
     5341 tatattgttt catgtaaagg tcattatgat gattgaaaaa tcatattgac aaatcaccca
     5401 atgcacttta atattgtaga aagaaacagg caaatattta aaggcataag aaagaggagt
     5461 acatgacgaa agctttcaga gagagcatcc ggtccgtaag atggtgatac attttgggca
     5521 ggctgaaaga tgcttaagac agaaatcatg gaaggtagct tttgagccga agaacccaga
     5581 aagttaaatc tatatataac gagatttgac ataagttctt ccgattcgtc cacgttacag
     5641 gacaagaggc attcgagcgt atatgtcata tattttagtg tatatgtcgc tctgatgtaa
     5701 acgaaggtgg tacagcgctt attcgccctt ccggtcgtag accggagggg cgttttttgt
     5761 agactgagat gtgcacatat agcgcaaatt tatttgctac tatgctattt tcgtcatggc
     5821 atgtactaaa actgtaagac cggcagattg cgcatgcaat ctaatcggtc gccagatgaa
     5881 aagaaaaaca gcaaaagctc acagcctgaa ttatcaggcc gcgcattatg tatggaatta
     5941 tgtgatttat ggagctgcct agagactgtt aaaattatgt acacagccca gcaacgcacg
     6001 cacatggacg tgcgggcgag gcgttagcgc catggacggc gctaaaagcc ggttgcgtca
     6061 tggtctaagc aatccctaat cataattttt acagtgtctt ggcagcgtaa tcatgaacat
     6121 aattccatac ataatgcgcg acctgacaca acaaagcccc caaagctttt gcgtcattca
     6181 tataaaagga ggaaaacatg agcaaagaac taaacaaaac ctacgatcca aaggacatcg
     6241 aggaccgtct ttacaaaaag tgggaagaca atggctactt ccatgctgag gtagaccgca
     6301 gcaaaaaacc attcacaatc gtgatgccac caccaaacat cacaggacag ctgcacatgg
     6361 gacatgccct tgacaacacc atgcaggata tccttatcag atacaagaga atgcagggct
     6421 acaatgcact ctggcagccg ggaacagacc acgcatccat cgcaacagag gtaaaggtca
     6481 tccaggcact taaagagcag ggaatcaaca aggcagacct tacaagagaa gagttcctcg
     6541 agaaatgctg ggactggaga aaagagtacg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnng tacaggaggt atttacaaag ctttataaga agggctggat
     6721 ttataagggc tcaagaatcg taaactggtg tccggtatgt aagacatcta tttcagatgc
     6781 agaggtagag catgaggagc aggacggctt cttctggcac atcaactatc ctgtagttgg
     6841 cgaggaaggc agattcgtag agatagcaac aacacgtcct gagacacttt tcggagatac
     6901 agccgtagct gtaaaccctg atgatgagcg ttatcaggat atcatcggaa agaccttaaa
     6961 gcttccatgc acagacagag agattcctgt aatcgcagat tcatacgtag ataaagagtt
     7021 tggaacaggc tgtgtaaaga ttacacctgc acacgaccct aacgatttcg aggtaggaaa
     7081 acgccacagc ttagaggaaa tcgttgtaat caatgatgat gccacaatga atgaaaaggc
     7141 cggaaaatat gccggaatgg acagatacga gtgtagaaag gcactggttg aggaccttaa
     7201 ggagcagggc cttttagtaa aggttgtacc acactcacac aatgtaggtg tgcatgaccg
     7261 ctgtcacaca acagttgagc caatgatcaa gcagcagtgg ttcgtcaaga tggacgagat
     7321 gatcaagcct gcagttgagg gagtaaagaa cggagaaatc aagctccttc cttcaagaat
     7381 ggacaagaca tacttcaact ggacagacaa tatccgcgac tggtgcatca gccgtcagct
     7441 ctggtgggga cacagaatac ctgcatggta ctgtgatgac tgtggagaga tggtagtttc
     7501 aatagaaaag cctggcaaat gccctaagtg tggcaaggag cactggacac aggatcctga
     7561 tacacttgat acatggttct catcagcgct ctggccattc tcaacactcg gnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat
     7741 ggactcgtaa gagattctca gggccgcaag atgagtaaat cactcggaaa cggaattgat
     7801 ccgcttgagg ttatcgacaa gtatggtgca gatgcacttc gtctgacact tatcacagga
     7861 aatgcaccgg gaaatgacat gcgtttctac tgggagagag ttgaggcttc aagaaacttc
     7921 gccaacaagg tatggaatgc atcaagattt atcatgatga accttgaagg tgtagagctt
     7981 agagagccaa agcttgatga acttcatgca gcagacaagt ggattctttc aagggtaaat
     8041 acacttgcaa aggatgccac agaaaacatg gacaagttcg agcttggaat tgcagtacaa
     8101 aaggtatacg acttcatctg ggacgagttc tgtgactggt atatcgagat tgcaaaggtt
     8161 cgtacctaca agaaggatga aaacccagag tcagcaaatg cagcactttg gacgcttaag
     8221 acagtacttg taaatgcact gaagctcctt catccatata tgccgtttat cacagaggaa
     8281 atcttctgca cacttcagtc agacgaggag actatcatgc tctctaaatg gcctgagtac
     8341 aaggaagagt ggaatttccc ggctgaggaa gcagcaatcg agcactgcaa ggacctcgta
     8401 aagggtatca gaaatgtccg tacacagatg gatgttccac cttcaagaaa ggcaaagcta
     8461 ttcatcacaa gtgatgatga ggcagtcaga aaggtattcg aggacaacaa ggaggtatat
     8521 gtaaatcttg catttaccag cgagattgca gtacagcagg gcaagtccgg tattggagat
     8581 gacgctgtat cagtagtgat acctgatgcg gttgcatacc tgccgcttga ggaccttgtt
     8641 gatttcgaga aggaaaagga aagactcaat aaagaaaagg ataagctcac aaaggagctt
     8701 gcgcgctcaa ggggcatgct ctcaaatgag aaattcttaa acaatgcaaa gcctgaaaag
     8761 gttcaggagg aaaaggataa gcttgcaaag tatgagcaga tgatggctca ggtagaggaa
     8821 cgtctggctc agtttaaata attaaataca gattatattt tgtgaacact gttttcctgg
     8881 ggcaaggatg caaaggaggc cgtccacaat gcggtggttc ttgaatagaa tagaacaaag
     8941 aaagaa
//
LOCUS       DPUF01000012            3299 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7071, whole
            genome shotgun sequence.
ACCESSION   DPUF01000012 DPUF01000000
VERSION     DPUF01000012.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7071"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(12..1181)
                     /locus_tag="DHV81_00365"
     CDS             complement(12..1181)
                     /locus_tag="DHV81_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCI93007.1"
                     /translation="MKIYVGIDIAKLNHFAAAISSDGEIIIEPFKFTNDADGFQLLVS
                     KLESFDKNSLIIGLESTAHYGDNLVRYLVTELYQVCVLNPIKTCQMRKNNVRKTKTDK
                     VDTYVIAKTLMMQDNLRFVSFFDLDMMDLKALGRFRQKTIKQRTRLKIQLTTYVDQVF
                     PEIQYFFKSGLHQHAVYALLKEAPSPKEIASMHMTHLANLLKVNSHGHFTKEQAKELR
                     VLAQKSVGANDSAISIQITQTIQQIELLDSQLEKIEAEMTDIMKFNDSVIMTIPGIGY
                     INGGMILGEIGDIHRFSNPNKLLAFAGLDPSVYQSGNFQAKTTRMSKRGSRVLRYALV
                     NAAWNVVRNNATFKAYYDAKRAEGRSHYNALGHCAGKLVRVIWKMLTGEVEFNLE"
     gene            1458..2996
                     /locus_tag="DHV81_00370"
     CDS             1458..2996
                     /locus_tag="DHV81_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCI93008.1"
                     /translation="MRISDIMRLGKSAIIFATIVVAFLAIIWLLGYKMIYKKILHGKK
                     QISIGRIGLVCVLAVYIVVVIYVTLLRGGIGFGGFEYRANFKPFSSYKEAWYNFSAQE
                     WRNLILNICMFVPFGFLLPICFGKIKRAWKIYLCGFGFALFIEVVQLITGRGVFETDD
                     IINNTIGAMIGYGLFSVARLIFVAVCSRKKVQDNTNEVSGVAHDENVCERKNISIRKC
                     LVAQLPLAFTIIAFAAVFIVYNSMEYGNLSIDNISNQNVDVSMADGVSLADEADPLDV
                     YTIHRATEDEARELADGYFSKYGVLIDDSKTDIYDDTIIFYSTSLDDEGSNLSIWCDY
                     EGPTVSFTDFSNIDDENSYADAGLSEEFVREKLENLGVVIPENAVFAPIEEYDAGNYR
                     FTNDGEILDDGLYYKGTIECCINSSGKIANFRDSMIKYTPYKKVDVISEKEAYDRLCA
                     GKFYFPDYDKDEQLSDLVVKSVKISYTPDSKGYYRPVYEFVANANQDTGKREISIMVD
                     AMEI"
     gene            3131..>3299
                     /locus_tag="DHV81_00375"
     CDS             3131..>3299
                     /locus_tag="DHV81_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI93009.1"
                     /translation="MLLNNNSNGNYTMRQLKLPLEIEKLIDISDPVYTFCEVMDHIDL
                     SRYFVEKGYKTG"
BASE COUNT         1038 a          540 c          736 g          985 t
ORIGIN      
        1 atacagacct cttattcgag gttgaattct acttcgccag tgagcatctt ccagatgact
       61 ctgacaagct tgccggcaca gtgcccaagt gcattgtagt gagaccggcc ttcagccctc
      121 ttggcatcat aataagcctt gaaggttgcg ttgtttctga caacgttcca agctgcattt
      181 acaagggcat atcgtaaaac acgagagcca cgtttggaca tccttgttgt cttagcctga
      241 aagttaccag actgataaac agaaggatcc aaaccggcaa aagcaagcag cttgttagga
      301 ttggagaaac ggtgaatatc acctatttca ccaagaatca ttccaccatt gatataaccg
      361 ataccaggaa tggtcatgat gacagaatcg ttgaatttca tgatatccgt catctcagct
      421 tcaatctttt ctaattggct atccagtaac tcgatttgtt gaatggtttg agttatctga
      481 atagatatag cgctgtcgtt agcaccgaca gacttctgtg cgagaactct taattctttg
      541 gcctgttctt tggtaaagtg tccgtgtgag ttcactttga gcagatttgc cagatgagtc
      601 atatgcatgg aagcaatctc ttttggagaa ggtgcttctt ttaataaagc atagacagcg
      661 tgttgatgca gaccggattt gaaaaagtat tgaatctccg gaaagacctg atcaacatag
      721 gttgtcagtt gaattttcaa tcgggtacgt tgctttatgg ttttctgacg gaaacgtccc
      781 aatgccttaa gatccatcat atcgagatcg aagaagctga cgaatctgag gttgtcctgc
      841 atcataagag ttttagcaat cacgtaagtg tcgaccttat ctgtcttagt tttgcgaacg
      901 ttattttttc gcatttgaca ggttttgatg gggttcaaca cacacacttg gtaaagctca
      961 gtaacaaggt atcgaacaag gttgtcaccg tagtgtgccg ttgactcaag accgatgatg
     1021 aggctgttct tatcgaatga ttcgagttta gagaccagca gttggaagcc atcagcgtca
     1081 tttgtgaatt tgaacggctc aatgattatt tcaccgtcgg aagaaatcgc agcggcgaaa
     1141 tgattaagtt tggcaatatc aatgcctacg taaattttca tgaggttgta ccgccctttc
     1201 ctaaagtctg ataccatgat gtccaccaac gattatcatc gtagacttga tagaaataag
     1261 tactctgagt gaaaaacact ccggcaacat ccagctgata aacaattcag ataaaggtag
     1321 tggcaataca ctccagtcga gtagtcaagc tacagaaaaa aatcaaaagt ccatagtatc
     1381 tgaattgtat tgaaccacga tattaaaaga aaggaaacgt gtgataatta acttcctaat
     1441 tatcatacaa gaatgtaatg agaatatcag atattatgag attaggaaag tctgccatta
     1501 tttttgccac tattgtagtg gcttttttgg caataatctg gcttttaggc tacaaaatga
     1561 tttataaaaa aatattgcat ggtaaaaaac agatttcaat tggcagaata ggactggtgt
     1621 gtgtacttgc agtatacatt gttgttgtca tttatgtgac attgctaaga ggaggtattg
     1681 gttttggggg atttgagtac cgggctaact ttaaaccatt ttcttcctac aaagaggcct
     1741 ggtacaattt tagtgcacag gaatggagaa atctgatact taatatatgt atgttcgtgc
     1801 cgtttggatt tttactacca atttgttttg gaaagattaa aagagcgtgg aaaatatatt
     1861 tgtgcgggtt tgggtttgca ctgtttatag aggttgtgca gcttatcacg ggacgaggtg
     1921 tatttgaaac tgacgatatc ataaataata cgataggtgc aatgatcgga tatggattat
     1981 tttcggttgc aagactgata tttgtggcag tctgttcaag gaagaaagtc caagataata
     2041 caaatgaagt cagtggagtg gcacatgatg aaaatgtttg tgaaagaaag aacatttcca
     2101 taagaaaatg tctggtggca cagcttccgc ttgcgtttac aattatagct tttgcagctg
     2161 tttttattgt atacaatagt atggaatatg gtaatctttc aatcgataat atttcaaatc
     2221 aaaatgtgga tgtctcgatg gcagatggtg tgagtctggc agatgaggca gatccgcttg
     2281 atgtatacac aattcacagg gctacagaag atgaggcgag ggagcttgcc gacgggtact
     2341 tttcaaagta tggtgtttta atagatgact caaaaacaga catatatgat gatactatta
     2401 ttttttacag taccagcctt gatgatgagg gcagtaacct gagtatctgg tgcgattacg
     2461 aaggaccaac agtaagtttc acggattttt caaatataga tgatgaaaat tcgtatgctg
     2521 acgctggact ttcagaggaa tttgtcagag aaaagctgga aaatctagga gtggttattc
     2581 ccgaaaacgc agtttttgca ccaatcgagg aatatgatgc aggaaactat aggtttacaa
     2641 acgatggaga aattcttgac gatggcttgt attataaggg aacgatagaa tgttgtatta
     2701 attcgtcagg taaaattgca aatttcagag actccatgat aaaatatacg ccttataaaa
     2761 aggtagatgt tatttcagaa aaagaggctt atgacaggct atgtgcagga aaattttatt
     2821 tcccggatta tgataaagat gaacaattat ctgatttagt tgtaaaatca gtgaagataa
     2881 gttacacacc ggattcgaag ggatattatc gtccggttta tgagtttgtg gcaaatgcaa
     2941 atcaggatac aggcaagaga gaaataagta taatggtaga tgctatggaa atttagggag
     3001 gaataactta caaaaattaa ctgacagctg cattaggtct ggtaaaaaac aaaaggggct
     3061 gtaaaaaaac tcccctgaca gaaaatcgcc cagaccgtga ggtctgagcg attttttggt
     3121 ataattaatt atgctactaa ataataatag caatggtaat tatactatgc gtcagctgaa
     3181 attaccatta gaaatcgaaa aattgatcga tatttccgat ccggtataca ctttttgtga
     3241 ggttatggat cacattgacc tatccagata ttttgtagag aaaggctata aaacaggtc
//
LOCUS       DPUF01000013            4593 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1606, whole
            genome shotgun sequence.
ACCESSION   DPUF01000013 DPUF01000000
VERSION     DPUF01000013.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1606"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(64..249)
                     /gene="rpmB"
                     /locus_tag="DHV81_00380"
     CDS             complement(64..249)
                     /gene="rpmB"
                     /locus_tag="DHV81_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCI93010.1"
                     /translation="MAKCAICEKGVHYGNNVSHSHRRSNHVWKSNIKKVSCKVNGVPQ
                     KLYVCTSCLRSGKVERA"
     gene            424..783
                     /locus_tag="DHV81_00385"
     CDS             424..783
                     /locus_tag="DHV81_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCI93011.1"
                     /translation="MKGQMDTQYGQILIDTDVIATYAGSVAVECFGIVGMAAVNMKDG
                     LVKLLKRDYLNHGINVKVDENNEITIDFHVIVAYGISIGTVSDNLIETVKYKVESFTG
                     MKIAKINIYVEGVRVID"
     gene            838..>1169
                     /locus_tag="DHV81_00390"
     CDS             838..>1169
                     /locus_tag="DHV81_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HCI93012.1"
                     /translation="MFLAGAKNLDSKKDWINELNVFPVPDGDTGTNMTMTIMSAAKEV
                     SSLTEPTMAELAKAISSGSLRGARGNSGVILSQLFRGFCKVIKEYDEIDVTILCEACQ
                     KAVETAYKA"
     assembly_gap    1170..1336
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1337..2494
                     /locus_tag="DHV81_00395"
     CDS             <1337..2494
                     /locus_tag="DHV81_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HCI93013.1"
                     /translation="QAGVVDSGGQGLVQVLKGAYDALIGKEIDYTIEGAPTGAAPAKI
                     SAETEAEIKFGYCTEFIIVLNAPMSDNEEHAYKAFLESIGDSIVVVADDEIVKTHVHT
                     NDPGLALQKALTFGSLSKIKIDNMREEHQEKLIKDSQKLAAQQKAEEEAYEAAQADEK
                     TNNMPAKEMGFVSVSIGEGMNEVFRGLGVDYLIEGGQTMNPSTEDMLNAIEHVNAKTV
                     FILPNNKNIIMAANQAVDLVEDKQIIVIPTKTIPQGVTALVNYIPDHSAEENKEQMMA
                     EIENVKTGQVTYAVRDTEIDGKTIKQNDFMGIGDKSILSVGTDLKATTLEMVDAMVDE
                     DSAIVSIYFGSDSDEDSANELAAAIEEKYPDVEVEVNDGGQPIYYYVISVE"
     gene            2542..>4593
                     /locus_tag="DHV81_00400"
     CDS             2542..>4593
                     /locus_tag="DHV81_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HCI93014.1"
                     /translation="MDIASPITAVKGIGEKTQKAFAKMGVYTVGDILLHFPRDYVKFP
                     EITDIDELNNVNVSSTYAIHAVIKKAPVVKNTARMQITLQDIGSPGHMIQLVWYRMPY
                     LKAQLVQGRHLIFYGHIKPKGGRYVMEQPVVYEVQKYEAIRDTLQPVYSVTSGVTNNL
                     IVKTIKETLSHDTLLSDYLPKDIRHRYGFCEYNYAMKQIHFPDDFDALVEARRRLVFD
                     EFFLFIMGMRYQNKKQQKDKNEFEFTDDAFIDGLIDKLPYELTGAQKKTIDEIKQDIK
                     SPYVMQRLIQGDVGSGKTIVAFLLMAWASKCGYQSAIMAPTEVLANQHYETFCSLVSQ
                     FGLDSPVVLLTGSMTAKQKREAYARLENEKNALIIGTHALIQEKADFSNLSLVITDEQ
                     HRFGVKQRESFSDKGRKPHILVMSATPIPRTLAIIIYGDMDISVINEVPAKRLPIKNC
                     VVGTAFRPKAYSFIAEQVRAGHQAYVICPLVEETENSEGENVTDYANVLKAALPKDIT
                     VDVLNGRMKSKAKDEVMQRFANNESQVLVSTTVVEVGVNVPNATVMMIENADRFGLAQ
                     LHQLRGRVGRGDAQSYCIFINSSNSKKSKKRLEILNKSNDGFYIASEDLKLRGPGDFF
                     GIRQSGELAFALADVYQDSDVLKEASEMVDEVLEADPALCAAENRILKKKMDEFAKEQ
                     LK"
BASE COUNT         1402 a          802 c         1104 g         1118 t
ORIGIN      
        1 gagcaaataa aaaggacatc caaacggatg tcctttaaaa ttcaaaagat tcaaaaataa
       61 agactaagct ctctcaactt tgccagatct taaacatgaa gtacatacat ataacttctg
      121 tggaactcca ttgaccttgc aagaaacctt cttaatgttt gacttccaaa catgatttga
      181 tcttctatga gaatggctga cattatttcc ataatgaaca cctttttcac aaattgcaca
      241 ttttgccatg atattgcacc tccttgcatt tcactaagct taccatactc ttaagctcac
      301 aacaaatgta ttctatcaga ttaaaataaa gaatgcaagg ataaaatata aaaatatttg
      361 ctttttgtga aaaaaaaggt ataataacat tagctacgtc acaatatgat ggaggtatat
      421 attatgaagg gacagatgga tacccaatat ggacaaattt taattgatac agatgttatt
      481 gcaacctatg caggctctgt agctgtagag tgctttggaa tagtaggcat ggcagcggtc
      541 aacatgaagg atggtcttgt aaagctttta aagagagatt acctgaacca tggaataaat
      601 gtaaaggtag atgagaacaa cgaaattacc attgattttc atgtaatagt agcgtatggt
      661 atttcgatcg gcactgtttc ggacaatctt atcgagacgg ttaagtacaa ggttgagtca
      721 tttacaggaa tgaaaattgc aaagatcaat atttacgttg agggcgtaag ggtaattgat
      781 taaggaggac tacaaaaagt ggagatcaca agcattaatt caaagctcct tgcaaggatg
      841 tttcttgcgg gagccaagaa tcttgattca aagaaagact ggataaatga gctgaatgta
      901 ttcccggtac cggatggtga taccggaaca aatatgacaa tgacaatcat gtctgcggcc
      961 aaggaggtca gctcgctcac agagcctacc atggcagagc ttgcaaaggc tatttcatca
     1021 ggctctttaa gaggcgccag aggaaactcg ggtgttatct tatcacagct tttcagaggc
     1081 ttctgcaagg ttataaagga atacgatgag atagatgtca ctattctttg tgaggcatgc
     1141 cagaaggcgg ttgagacagc ttacaaggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnncagg caggtgttgt ggattcgggc ggtcagggac ttgttcaggt
     1381 attaaaggga gcatatgatg ctcttatcgg taaggagatt gactatacga tagagggcgc
     1441 accaacagga gcagctccag caaagatttc ggctgagaca gaggcagaga ttaagtttgg
     1501 ttactgcaca gagtttatta ttgtactcaa tgcgccaatg tcagacaatg aggagcacgc
     1561 atacaaggca ttcctcgagt caatcggtga ctctatagtc gttgtagctg acgatgagat
     1621 cgtaaagact cacgtacata caaacgaccc tggacttgca ctgcagaagg cacttacctt
     1681 cggctcgctc agcaagataa agatcgacaa tatgcgtgag gagcatcagg aaaagctcat
     1741 caaggattca cagaagcttg cagcacagca gaaggctgag gaagaggctt atgaagctgc
     1801 tcaggctgat gaaaagacaa ataacatgcc tgcaaaggaa atgggctttg tatctgtttc
     1861 aatcggagag ggcatgaatg aggtgtttag aggccttgga gtggactatc tcatagaggg
     1921 tggtcagacc atgaacccta gtacagagga tatgctcaac gctatcgagc atgtaaatgc
     1981 aaagacagta tttatcctgc caaacaacaa gaacatcatc atggctgcaa accaggcagt
     2041 agaccttgtt gaggataaac agattattgt tatccctaca aagactatcc cacagggtgt
     2101 cacagcgctt gtaaattata tacctgacca cagtgccgag gagaataaag agcagatgat
     2161 ggctgagata gaaaatgtca agacgggaca ggtgacatat gcagtccgcg atactgagat
     2221 tgacggaaaa accatcaagc agaacgactt catgggaatc ggagacaaga gcatcctttc
     2281 tgttggaaca gacttaaagg ctaccacact tgagatggta gatgccatgg tagatgagga
     2341 ttcagcgatt gtcagcatct atttcggaag cgattctgat gaggactcag ctaatgagct
     2401 tgcggcagca atcgaagaga aatatcctga tgtagaggtt gaggtcaacg atggcggaca
     2461 gccaatctac tattatgtta tttctgtaga gtaaagcctt ttaaagaata taaatgatat
     2521 ttattatatt taggagcaaa tatggatata gcttcaccga ttaccgcggt taagggaatc
     2581 ggggaaaaaa cacaaaaagc atttgctaaa atgggagtat acaccgttgg tgatatactc
     2641 cttcattttc cccgggatta tgtaaagttt ccggaaataa cggatattga tgaactgaat
     2701 aatgtaaacg taagcagcac ctatgccatc catgctgtga ttaaaaaggc acctgtagtg
     2761 aaaaatacag cgagaatgca gattacattg caggatatcg gctcgcccgg gcatatgata
     2821 cagctggtat ggtaccgtat gccatacctt aaggcacagc ttgtgcaggg aagacatctt
     2881 atattttacg gacatattaa gccaaagggc ggcagatatg tcatggagca gcctgtggtg
     2941 tatgaggtgc aaaagtacga ggctatacgc gatacactgc agcctgtgta ctccgtgaca
     3001 agcggtgtga caaacaatct gatagtgaag acgataaagg agactctgtc acatgataca
     3061 ctgctgtcag attatctgcc taaggatata aggcacagat acggcttttg cgagtataat
     3121 tatgccatga agcagataca tttccctgat gactttgacg ctcttgtgga ggcaagaaga
     3181 cggcttgttt ttgatgagtt ttttctcttt atcatgggca tgcggtatca gaataaaaag
     3241 cagcagaagg acaaaaatga gtttgagttt acggatgatg cttttataga cggactgatt
     3301 gataagcttc cgtatgagct gacaggcgcg cagaagaaga caatagatga gataaagcag
     3361 gatattaaaa gcccttatgt gatgcagagg cttattcagg gcgatgtggg ctcaggaaag
     3421 acgattgttg cttttcttct tatggcgtgg gcgtcaaaat gtggctatca gtctgctatt
     3481 atggctccga cggaggtgct tgcaaaccag cattatgaga cgttttgctc actggtcagt
     3541 cagtttggac ttgatagtcc tgttgtgctt cttaccggct ccatgacggc aaagcagaaa
     3601 agagaagcct atgcgcgcct tgaaaacgag aaaaatgcgc ttattatagg cactcatgcg
     3661 ctcatacagg agaaagcgga tttttccaat ctgtcgcttg ttatcacaga tgagcagcac
     3721 agatttggag tgaagcagag ggagagcttc tcggataagg gaagaaagcc acatatactt
     3781 gtgatgagtg ccactccgat accgcgtacg ttagcaataa tcatatacgg tgatatggat
     3841 atctctgtca taaatgaggt gcctgcaaag aggcttccaa tcaagaactg tgtggttggc
     3901 acggcattca ggccaaaggc ctacagcttt attgctgagc aggtgagagc cggccatcag
     3961 gcgtatgtta tatgtcctct tgtggaggaa accgaaaact cggaagggga aaatgtcaca
     4021 gactatgcaa atgtgcttaa agcggcactg ccaaaggata tcactgtaga tgtgcttaac
     4081 ggcaggatga agagcaaggc aaaggatgag gtgatgcagc gctttgcaaa taatgagtcg
     4141 caggtgcttg tgtccacaac agtcgtggag gttggtgtaa atgtgcccaa tgcaaccgtc
     4201 atgatgatag aaaatgcaga caggtttgga cttgcacagc ttcatcagct aagaggccgt
     4261 gtaggcaggg gggatgcaca gtcgtattgc attttcatca attcgtcaaa ttcaaagaaa
     4321 tcaaaaaaac gtctggagat actaaataaa tcaaatgatg gcttttatat agcaagtgag
     4381 gatttaaagc tcagaggtcc cggggatttc tttggaatca ggcagagtgg agaacttgcg
     4441 tttgctcttg cggatgtgta tcaggatagc gatgtgctaa aggaagcatc tgagatggtg
     4501 gatgaggtgc ttgaagcaga cccggcgctt tgtgcagcgg aaaacagaat actgaaaaag
     4561 aaaatggatg aatttgcaaa agaacaatta aag
//
LOCUS       DPUF01000014            5169 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_427, whole
            genome shotgun sequence.
ACCESSION   DPUF01000014 DPUF01000000
VERSION     DPUF01000014.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_427"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="DHV81_00405"
     CDS             complement(<1..758)
                     /locus_tag="DHV81_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HCI93015.1"
                     /translation="MALFEAYERRIDQINAVLNSYGISSIEEAEKITKDAGLDVYDQV
                     KKIQPICFENACWAYTVGAAIAIKKGCRRAADAAAAIGEGLQAFCIPGSVADHRKVGL
                     GHGNLGKMLLEEETECFCFLAGHESFAAAEGAIGIAEKANKVRQKPLRVILNGLGKDA
                     AQIISRINGFTFVETQYDYKAAKLNIVSEHAYSDGLRATVKCYGADDVQEGVAIMHHE
                     NVGVSITGNSTNPTRFQHPVAGTYKKECIEQGKK"
     gene            complement(777..1478)
                     /locus_tag="DHV81_00410"
     CDS             complement(777..1478)
                     /locus_tag="DHV81_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93016.1"
                     /translation="MIYTKEVENMCPVAKGAKHEPAPIPEEGKWVHSKKIEDISGFTH
                     GVGWCAPQQGACKLSLNVKNGVIEEALVETIGCSGMTHSAAMAAEILQGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRTQSAFSDDGLAVGAGLEDLGKGLRSQVGTMYATKAK
                     GVRYLEMAEGYVTGIALDKNDEVIGYQFVSLGKMTDFIKKGDDANTAWEKASGSYGRV
                     TPEQGAVRIIDPRQE"
     gene            complement(1601..2275)
                     /locus_tag="DHV81_00415"
     CDS             complement(1601..2275)
                     /locus_tag="DHV81_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HCI93017.1"
                     /translation="MENAKIINLYNLDETIAKEYLSQFTYPWEALAGISDFIKELGPT
                     LDPEIYEKRGEDIWVAKSATVFDSAYLHGPLIICEDAEIRQCAFIRGSAIVGKGSVVG
                     NSTELKNVIIFNSVQVPHYNYVGDSILGYKSHMGAGSITSNVKSDKTLVVVKDVYDTK
                     EEIETGRKKFGAMLGDHVEVGCNSVLNPGTVIGRNSNIYPLSRVRGVIPANHIFKDKD
                     NVVLKK"
     gene            complement(2418..>5169)
                     /locus_tag="DHV81_00420"
     CDS             complement(2418..>5169)
                     /locus_tag="DHV81_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCI93018.1"
                     /translation="LKGFEPTADFVEHIMQKRLYANIADTQYTDDSKVTDHYAIIPTG
                     QLTELSGLTSLQRAVFELIVRRFLSIFYPPAEYENVKLVVDVDKESFFASSKVLKSEG
                     YLQVANPPKKKSFEESNARILGKNTETADSTDENSTANGSNDEIVVNPKVMLELVDKL
                     KAGDEIACQGYNIKFGKTSPPKRYTSGSMVLAMENAGQLIEDEELREQIKGSGIGTSA
                     TRAEIIQKLVRIGYLNLNKKTQILTPESLGEMVFEVVYMTVPALLNPKMTANWEKGLD
                     GITRGTVDFWEYRGKLESFIRKETEKMIEQNLRPEIADRISSFAGKNARGAAARRKIG
                     VKCPVCGGEIVTTPFGYGCANYKNDKSGCNFNIGQVAGLDIPEEQVKKLIEEGITDTI
                     RGFKSKSGKRFDACLKLNKDEQTNKVSATFDFENVKPQKVEGIVCPDCGGDIYVSPYG
                     YRCANYNRQTEDGCHFFVGRIADKQLSQVQFLELMANGRTETIKGFKSKSGKRFNACL
                     VLKKEPVTEKSEKMHTVVAFDFDNVEDEIIKDVVCPLCGGQIKKTSFGYGCVNYKGDE
                     PGSCRFSVGQIAGKTITAANLKQLLNDKKTETIRGFKSKSGKRFDACLVLKEDENKKL
                     SVQFDFDHVEPKTVKDVVCPKCGGKIQVAPFGFVCENNRRDDPESCRFMVGKVASVKI
                     KESVLKELLTKKKTEVISGFVSKTGMKFDAPLKLTEDGDIAFDFPEKPKPIETNVLCP
                     KCGRPLKKTQWKYECECGFNVWHTVAKVELSEEILTELFTTGKTKKKVTGFTSKAGNA
                     FDTCLKFENDQISFDFDNPGEEEVQSTAEVHNNAEVHNNADAQSNADAYNNADTQSNT
                     DAHNNAEVQSTADDAPDETTLNEMGIPEEFLAGFDMAADSVQADDVSEAALAAAHELF
                     GE"
BASE COUNT         1206 a         1319 c          969 g         1675 t
ORIGIN      
        1 tacttcttgc cctgctcgat acactctttc ttgtatgtac ctgctaccgg atgctggaaa
       61 cgagttgggt ttgtagagtt tcctgtgata gaaacaccaa cgttctcgtg atgcatgatt
      121 gcaacacctt cctgaacatc atctgcaccg taacacttaa ctgttgcacg gagtccatct
      181 gagtatgcat gctcagatac gatgttgagc ttagctgcct tgtagtcata ctgtgtctct
      241 acgaatgtga atccgttgat acgtgagatg atctgtgcag cgtcttttcc aagtccgtta
      301 aggataacac gcagtggttt ctgacgaacc ttgtttgcct tctcagcgat tccgatagca
      361 ccctcagcag ctgcgaatga ctcgtgacct gctaagaaac agaagcactc tgtctcctcc
      421 tcgaggagca tctttccaag gttaccatgt cctaaaccta ccttacggtg atcagcaact
      481 gatccaggga tacagaatgc ctgaagtccc tctccgattg ctgctgcagc gtctgctgct
      541 cttctacatc cttttttgat tgcgattgct gcaccaactg tgtatgccca gcaagcgttc
      601 tcgaaacaga ttggctggat tttctttacc tgatcgtaaa catcaagtcc agcgtccttt
      661 gtaatctttt cagcttcttc gattgagctg ataccatagc tgttaagtac agcattgatc
      721 tggtctattc ttctttcata tgcttcaaat aatgccattg tcgttctcct ttcctcttat
      781 tcctgtcttg gatcgatgat cctaacagca ccctgctctg gagttacacg accgtatgat
      841 cctgaagctt tctcccaagc tgtgttagcg tcatcaccct ttttgataaa atctgtcatt
      901 tttccaagag aaacgaactg gtatccaatt acttcatcgt tcttgtcaag agcgattcct
      961 gttacatagc cctcagccat ctcaaggtaa cgaactccct ttgcctttgt tgcgtacatt
     1021 gtaccaacct gtgagcgaag tccctttcct aaatcctcaa gtcctgctcc aacagcaagt
     1081 ccatcatcag agaatgcaga ctgtgtacga ccgtaaacga tctgtaagaa taactctctc
     1141 atagctgtat tgatggcatc acaaactaag tctgtgttta atgcctcaag aattgtctta
     1201 ccctgtaaga tttctgctgc catagctgca gagtgtgtca ttcctgagca tccgatagtc
     1261 tcaactaatg cctcttcaat tactccattc tttacattga gtgagagctt gcaggcaccc
     1321 tgctgtggtg cacaccagcc tacaccgtgt gtaaatcctg aaatgtcctc aattttctta
     1381 gaatgaaccc attttccttc ttctgggata ggagctggct catgttttgc gcccttagct
     1441 acaggacaca tgttttcaac ttccttagtg taaatcatct taagactcct ttcgttatgg
     1501 ttttaacata cccgatttat tctctcatat tttttgtaat aagtctagag tatcatcgac
     1561 aaaaaacaac aaaaatacaa tattttttca aacaacatta ctatttttta agaactacgt
     1621 tgtccttgtc cttgaaaatg tggtttgcag ggatgactcc gcgtactctt gaaagcggat
     1681 aaatgtttga atttcttcct attactgttc ccggattgag cactgagttg cagcccacct
     1741 ccacatggtc accgagcata gcgccgaatt tcttgcggcc ggtttcaatc tcctcttttg
     1801 tatcgtaaac atcctttacg actacaagag tcttgtcact ctttacattt gatgtaatag
     1861 agcctgcgcc catatgtgat ttgtagccaa gaattgaatc tcctacatag ttgtagtgtg
     1921 gcacctgtac tgaattgaag atgattacat ttttaagctc tgtggagtta cctactactg
     1981 agccctttcc tacgattgca ctgccacgga taaaagcaca ctgtctgatt tctgcatcct
     2041 cgcagatgat gagtggaccg tgcagatatg cgctgtcaaa tactgttgcg ctctttgcta
     2101 cccagatatc ctcgcctctt ttctcataga tttcagggtc aagtgttggt ccgagctcct
     2161 tgatgaaatc acttattccg gcaagtgcct cccatggata agtgaactgt gataaatatt
     2221 cctttgcgat cgtttcatcc agattataga gatttattat tttagcgttt tccattttct
     2281 atgtcctttc ttttatttat tatgtaaaaa ttattgacta tttttttaac tgtcatttta
     2341 atatttaaac ttaaatataa gcattaaaca taaaaattca tattaattat agatttaata
     2401 attaattata ggcagaacta ctcaccgaac agctcatgcg ccgccgcaag tgccgcctcc
     2461 gacacatcat ctgcctgtac actatcagct gccatatcaa atcctgccag aaattcctct
     2521 gggattccca tctcattcag ggttgtctca tccggcgcat catctgccgt gctctgtact
     2581 tcagcattgt tatgtgcatc agtattgctc tgtgtatcag cattgttata tgcatcagca
     2641 ttgctctgtg catcggcatt gttatgtact tcagcattgt tatgtacttc agccgtgctc
     2701 tgcacctctt cctcacccgg gttatcgaaa tcaaatgata tctggtcatt ttcaaactta
     2761 agacatgtat caaatgcatt tcccgccttg gaggtgaagc ctgtgacctt ctttttggtc
     2821 tttcctgtgg taaacagctc cgtgagaatt tcctctgaaa gctccacctt tgccacagta
     2881 tgccaaacat tgaagccgca ctcacactca tatttccact gtgtcttttt gagaggtctg
     2941 ccacactttg gacagagtac atttgtctcg attggcttag gcttttcggg gaaatcaaag
     3001 gctatatctc cgtcttctgt gagctttagc ggtgcgtcaa acttcatgcc tgtctttgag
     3061 acaaatcctg atataacttc tgtctttttc tttgtcagaa gctccttaag gactgattcc
     3121 tttatcttga cgcttgcgac cttgcccacc ataaatcggc agctctcagg atcatcacgt
     3181 ctgttgttct cgcatacaaa tccaaacggc gccacctgaa ttttgcctcc gcacttcgga
     3241 catactacat ccttcactgt ctttggctct acatggtcaa aatcaaactg gacagagagc
     3301 tttttgtttt catcctcctt aagcaccagg cacgcatcga agcgtttccc gcttttggat
     3361 ttaaagcctc gtatggtctc ggtcttttta tcattaagaa gctgctttaa gtttgcggct
     3421 gtgattgtct ttcccgcaat ctgtcccacc gaaaagcggc agcttcccgg ctcatcaccc
     3481 ttgtagttca cacagccata gccaaacgat gtctttttta tctgaccacc acaaagcgga
     3541 cagacgacat cctttatgat ttcgtcctcc acattgtcaa agtcaaatgc tacgacggtg
     3601 tgcattttct cacttttctc tgtgaccggc tccttcttta aaacgaggca tgcgttgaag
     3661 cgctttcccg attttgattt aaagcccttt atcgtctctg ttctgccgtt tgccatgagc
     3721 tctaaaaact gtacctgtga cagctgcttg tctgctatcc ttccaacgaa gaaatggcag
     3781 ccatcctctg tctgtctgtt atagtttgca catcggtagc cgtatggcga cacatatata
     3841 tcgcctccac agtccgggca tacaatgccc tcgacctttt gcggctttac attttcaaaa
     3901 tcaaaggtgg ctgatacctt gttcgtctgt tcatccttgt tgagctttaa gcatgcatca
     3961 aagcgtttgc cgctcttcga cttaaaacca cggattgtgt cggtgattcc ttcttcaatc
     4021 agctttttga cctgttcctc cggaatatca agtcctgcca cctgtccgat attaaaatta
     4081 catcctgatt tatcattttt gtaattggcg cagccatagc caaacggtgt ggtcactatc
     4141 tcaccgccgc agaccgggca cttgacacct atctttcgtc tggccgccgc acctctggca
     4201 tttttgcctg caaagctgct gatgcggtct gcaatctcag ggcgcagatt ctgctcaatc
     4261 atcttttctg tctctttgcg gataaagctc tcaagctttc ccctgtattc ccagaaatcc
     4321 accgtgcccc ttgtaattcc gtccagtccc ttttcccagt tggctgtcat ctttggatta
     4381 agaagtgccg gaactgtcat gtatacgacc tcaaacacca tctcgccaag gctttctggt
     4441 gtgagtatct gtgtcttttt gttcagattc agatatccta tacggacaag cttctggatt
     4501 atctccgctc tcgttgctga ggtgcctatt cctgagccct ttatctgctc acgaagctcc
     4561 tcgtcctcga taagctgtcc tgcattttcc atggcaagca ccatggagcc tgatgtataa
     4621 cgctttggcg gagatgtctt accaaacttg atattatagc cttggcatgc tatctcatcc
     4681 cccgccttta gcttatcgac aagctcaagc atcaccttgg gatttacgac tatctcatca
     4741 ttgctgccgt ttgctgtgct gttttcatct gtactatctg cagtttcagt gtttttacca
     4801 agtatacgtg cgtttgattc ctcaaacgac ttctttttcg gcggattcgc cacctgtaaa
     4861 tagccctcgc tctttagtac ctttgatgat gcaaaaaaac tttccttatc cacatctacg
     4921 acaagcttca cattctcata ctctgccgga ggataaaaaa tactaaggaa acgcctgaca
     4981 atcagctcaa atactgctct ttgaagcgat gtaagccccg aaagctctgt aagctgacct
     5041 gtcggaatga tagcatagtg gtctgttacc ttgctgtcat ccgtgtactg cgtatcagct
     5101 atgttggcat atagcctctt ttgcataata tgctccacaa agtctgctgt cggttcaaaa
     5161 cctttaagc
//
LOCUS       DPUF01000015            4295 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23759, whole
            genome shotgun sequence.
ACCESSION   DPUF01000015 DPUF01000000
VERSION     DPUF01000015.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23759"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(192..277)
                     /locus_tag="DHV81_00425"
     tRNA            complement(192..277)
                     /locus_tag="DHV81_00425"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(240..242),aa:Ser,seq:tga)
     gene            complement(381..1100)
                     /gene="ispD"
                     /locus_tag="DHV81_00430"
     CDS             complement(381..1100)
                     /gene="ispD"
                     /locus_tag="DHV81_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCI93019.1"
                     /translation="MDKKKYTAIILSAGTGSRMKSNMPKQYMELLGQPVIYYSVKAFE
                     DSPVDEIVIVCSADYIEYFRHSIIEKYGFKKVKAIVQGGKERYNSVYEGLKAANGTDY
                     VLIHDGARPLVDNEIIVRSMHTVEKEKACVAGMPVKDTIKVSDELGYSANTPDRKSLW
                     QIQTPQCFEYDLLAQSYDKLFSDMSYGKSVPAITDDAMIVEYGSDTKVKLIEGSYENI
                     KVTTPEDMGIAELFLKKRRKM"
     gene            complement(1177..2214)
                     /gene="tsaD"
                     /locus_tag="DHV81_00435"
     CDS             complement(1177..2214)
                     /gene="tsaD"
                     /locus_tag="DHV81_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCI93020.1"
                     /translation="MTENTETKKEIKILAIESSCDETAAAVVVNGRDLRSNVISSQIA
                     LHTLYGGVVPEIASRKHIEKINQVIEQALSDAHMTLDDIDAIGVTYGPGLVGALLVGV
                     AEAKAIAYARNIPLVGVHHIEGHISANYIENKDLEPPFLALVVSGGHTHLVRVVDYGK
                     YEILGRTRDDAAGEAFDKVARAIGLGYPGGPKIEKVSHEGDPHSIEFPRAKIVGGVYD
                     FSFSGLKSAVLNYLNGCRMKGLEINQADVAASFQQAVTDVLVGHAENAIDEFKMDKFA
                     IAGGVASNGVIRHAMEEMCARKGVKFYRPSPILCTDNAAMIGAAAYYDFIAGKRSGLD
                     LNAVPNLKLGE"
     gene            complement(2244..2825)
                     /locus_tag="DHV81_00440"
     CDS             complement(2244..2825)
                     /locus_tag="DHV81_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93021.1"
                     /translation="MKKTGIVILTIALIAAICGSFYVVNDKSKRANQKEKVLTEVQRI
                     TTKDLDKNYPQTPREVVKLYNRIVKCYYGMQYSDEELDALTDQALKLFDDELAANNPK
                     DTYKQSVTADAQSYKEQGITLAQTGVCDSNDVKYVTDNGSKIAYVKASYFMKEGSSYS
                     KTYQEYVLRQDKEGCWRILTFYKIAADSDTETE"
     gene            complement(2837..3748)
                     /locus_tag="DHV81_00445"
     CDS             complement(2837..3748)
                     /locus_tag="DHV81_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCI93022.1"
                     /translation="MLDVCLLGTAGMMPLPNRWLTALSLKYNGSNILIDCGEGTQIAM
                     KEAGINFKPIDILCITHFHADHISGLPGLLLTMGNAERTEPLTIIGPKGLTRVVTALR
                     TIAPELPFEIKCIELNEQDEYFEMNGYHIHAFRVKHAVLCYGYSVEIKRSGRFSVERA
                     KEKEIPMRLWNPLQKGNTVEFEGRVYTPDMVLGPARKGIKVTYCTDSRPLEHIVEAAE
                     GSDLFICEGMYAEKEKIVKAKQYKHMTFYEAADMAKRAGVKELWLTHFSPSLVRAEDY
                     MPQVRDIFANAYLGKDAKSVELMFDED"
     gene            complement(3742..4191)
                     /gene="rimI"
                     /locus_tag="DHV81_00450"
     CDS             complement(3742..4191)
                     /gene="rimI"
                     /locus_tag="DHV81_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-protein-alanine N-acetyltransferase"
                     /protein_id="HCI93023.1"
                     /translation="MIIRPMEPEDADSVAQIEKDSFTQPWSRQGFLDAMRLPENIMLV
                     AQEDGEILGYQCTYVSFDEGELTNIAVKKSARGKGVGAHLIRCLQEKAKESGVERIVL
                     EARVTNEAAISLYQKMGFVNLGIRKNLYEHPVEDGVIMSYTAEENLC"
     gene            complement(4188..>4295)
                     /locus_tag="DHV81_00455"
     CDS             complement(4188..>4295)
                     /locus_tag="DHV81_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCI93024.1"
                     /translation="YEQGRAENAADHKPEYLRLSQAERERQEKERDFRI"
BASE COUNT         1015 a         1140 c          748 g         1392 t
ORIGIN      
        1 ttgtttgctc tcttgcgaca gctcgattat tctatcaaat cgctgttcgt ttgtcaagca
       61 cttttttatt tttttgaact ttgttttaag ttctctgtct cgcgacagct tgttcattct
      121 atcaaaatat ttttcatttg tcaagaactt ttttgttttc ttttgcatat ctgttttaaa
      181 gcaaaagaaa gcgcaggggg tgggattcga acccacgcgc ccttgcggac aaacggtttt
      241 caagaccgcc tcgttatgac cacttcgata cccctgcatg tcacaattaa tacttaattg
      301 tttagttgtc tgcacttgtt cagcgcagga tttatattat catcaatgtt acaatatgtc
      361 aacactaatt tctaattttt ttacattttt ctgcgtttct ttaaaaaaag ttctgcaatc
      421 cccatatctt caggagttgt gacctttata ttttcatacg agccctctat gagctttact
      481 tttgtgtcac tcccatactc tactatcatt gcatcatccg taattgccgg cacacttttc
      541 ccatatgaca tgtcactaaa cagcttatca taggactgag ccagcaaatc atattcaaag
      601 cattgcggtg tctgaatctg ccacaggctt ttcctgtcag gagtatttgc tgaatatccc
      661 aattcatctg acacctttat tgtgtccttt acaggcattc ctgctacgca tgctttttct
      721 ttttcaaccg tatgcataga acggactatt atttcattat ccaccagagg gcgtgcgccg
      781 tcatggatca gcacatagtc cgtgccgttt gctgctttaa gcccctcata tacagagttg
      841 tagcgctctt tgcccccctg cactattgct ttcaccttct taaagccata tttttcaatt
      901 atcgaatgtc tgaaatactc aatataatct gcactgcaga ctatgactat ttcatccacc
      961 ggactatcct caaatgcttt tacactataa taaatcacag gctgccccaa aagctccata
     1021 tactgctttg gcatattact tttcatccgg cttcctgtac cggctgacag tatgattgct
     1081 gtgtatttct tcttatccat attcctttat tttcctatcc atgataaatc tgcacattat
     1141 gtttgcaccc cggtgtaacc ggaagcaatt acagaatcat tcaccaagct ttagattcgg
     1201 aaccgcattc agatcaagcc cagacctctt gcctgctata aaatcatagt atgctgccgc
     1261 acctatcata gcagcattgt ctgtacacag tatcggtgac ggacgataga atttaacacc
     1321 cttgcgcgcg cacatttcct ccatcgcatg acggataaca ccattagaag caacgcctcc
     1381 cgcaatggca aatttgtcca ttttaaactc atctatggca ttctcagcgt gtccgacaag
     1441 cacatctgta accgcctgtt ggaacgatgc tgccacgtct gcctggttta tttccagtcc
     1501 cttcatcctg cagccgttta aataattgag tactgctgat ttaagcccgc taaagctgaa
     1561 atcataaact ccgcccacaa tctttgctct cgggaactct atgctgtgcg gatctccctc
     1621 atgtgaaacc ttttctatct taggaccgcc cggatagcca agtcctattg ctctcgctac
     1681 cttgtcaaag gcctctcctg ccgcatcatc cctcgtacgt ccgaggatct catatttgcc
     1741 gtaatctacc actctcacca gatgtgtgtg tcctcctgat acgacgagtg ccagaaatgg
     1801 tggctctaag tctttatttt ctatataatt tgcgctgata tgtccctcta tatggtgcac
     1861 tccgacaaga ggaatgtttc ttgcatatgc tatggccttt gcttctgcca cgcctacaag
     1921 cagtgctcct acaagccccg gaccatatgt gactcctatt gcgtctatat cgtcgagtgt
     1981 catatgtgca tctgaaagtg cctgctctat gacctgatta attttctcaa tatgctttct
     2041 ggaagctatc tccggaacta cccctccata taaagtatga agagctatct gcgatgaaat
     2101 cacattactt cgcagatcac gtccatttac tacgactgct gccgcagtct cgtcgcatga
     2161 gctttcgatt gcaagtattt taatttcttt ttttgtttct gtattttctg tcattttatt
     2221 acaccaatct taattatatt actctattcc gtttccgtat cagaatctgc cgcaatctta
     2281 taaaatgtaa gaatcctcca acagccttcc ttatcctgcc tgagtacata ttcctgatat
     2341 gtcttagaat acgagctgcc ctccttcatg aagtaagacg ccttaacata tgcaatttta
     2401 ctaccattat cggtgacata ttttacatca ttgctgtcac acactccggt ctgagcaagt
     2461 gtaatgccct gttccttgta gctttgcgca tctgccgtga cagactgttt gtaggtatcc
     2521 tttggattgt ttgctgcaag ctcgtcatca aaaagcttaa gcgcctggtc ggtgagtgca
     2581 tcaagctctt catcgctata ctgcatacca tagtagcact tcacaatcct gttataaagc
     2641 ttcaccacct cacgaggtgt ctgcggatag ttcttgtcga gatccttagt tgttattctt
     2701 tgtacctcgg tcagtacctt ttccttttga ttagcccttt tagacttgtc gtttacgaca
     2761 taaaacgaac cacatatcgc agcaatcagc gctattgtta aaataactat acctgttttc
     2821 ttcatatcgt ccctccttag tcctcgtcaa acatgagctc cacactcttt gcatcttttc
     2881 caagatatgc gtttgcaaaa atatccctca cctgtggcat ataatcctct gcacggacaa
     2941 gtgacgggga aaaatgtgta agccacagct ccttcacgcc ggctctcttt gccatgtctg
     3001 ctgcctcgta aaaggtcata tgcttatact gctttgcttt tactatcttt tccttttcag
     3061 catacatgcc ctcgcagata aaaaggtctg atccctctgc tgcctccact atgtgctcaa
     3121 gtggccttga gtccgtacaa tatgtgacct ttatgccctt tcttgccggt ccaagcacca
     3181 tatccggagt atacactctg ccctcaaatt caacggtatt gcctttctga agaggattcc
     3241 acaggcgcat cggaatttct ttttcctttg ctctttccac ggaaaatctg ccactacgct
     3301 ttatctcaac cgaatagcca tagcacagca ccgcatgctt tacgcggaat gcatggatat
     3361 gatatccgtt catctcaaaa tattcatcct gctcattgag ctctatgcat tttatctcaa
     3421 aaggcagctc cggcgcaatc gtccgaagtg cagtgacaac ccttgtaagc cctttaggtc
     3481 caataatggt aagcggctcg gtgcgctcgg catttcccat tgtaagaaga agccccggca
     3541 atccgctgat atggtctgca tgaaagtggg tgatgcataa aatatctatc ggcttgaaat
     3601 tgattccggc ctccttcatc gcaatctgcg ttccctcacc gcagtcaata agtatattgc
     3661 tgccgttata ttttagcgaa agtgccgtaa gccatctgtt tggcaaaggc atcatgcctg
     3721 ctgttccaag taaacacacg tctaacataa attttcctcc gcagtatagg acatgatgac
     3781 accgtcctct accggatgct catatagatt ttttctgatt ccaaggttta caaaccccat
     3841 cttctgatac agtgatatcg cagcctcatt agtcaccctg gcttcgagca caatgcgctc
     3901 aacacctgac tcttttgcct tttcctgcaa gcatctgata agatgtgcac ccacaccttt
     3961 tcctcgtgct gattttttga ctgctatatt ggtcagctct ccctcatcaa atgacacata
     4021 cgtgcactga taccccagta tctcgccatc ctcctgtgcc acaagcatga tattctccgg
     4081 aagcctcatg gcatccaaaa atccctgtct gctccagggc tgtgtgaagc tgtctttttc
     4141 tatctgcgcc accgagtctg catcctccgg ctccatgggt cttattatca tattctaaaa
     4201 tctctctcct tttcctgtct ctctctctcc gcctgtgata atctgagata ctcaggctta
     4261 tggtctgctg cattttctgc cctgccctgc tcata
//
LOCUS       DPUF01000016            2665 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_32466, whole
            genome shotgun sequence.
ACCESSION   DPUF01000016 DPUF01000000
VERSION     DPUF01000016.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_32466"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="DHV81_00460"
     CDS             <1..1305
                     /locus_tag="DHV81_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCI93025.1"
                     /translation="TGIATIAGTLIFMLTIDVKISCIVVLITPLSLFVASFITKKTYS
                     MFQLQTSTRGEQTSLIDEIVGNEKVVQAFNHEDEALEQFDEINDRLQKCSLRAIFFSS
                     LTNPCTRFVNSLVYAGVGIFGAMSALTGGITVGQLSCFLSYANQYTKPFNEISGVITE
                     LQNALACAARIFELIEEKKEIPDASDAVTLGEADGRVDIEDVYFSYVPDKKLIEDFNL
                     HVKPGQRVAIVGPTGCGKTTIINLLMRFYDVNSGTIKVSGHDIRKITRESLRDNYGMV
                     LQETWLKKGTIRDNIIMGKPDATDEEIIAAAKASHAHSFIRRLPKGYDTEIGEDGGSL
                     SQGQKQLLCITRVMLCLPPMLILDEATSSIDTRTEIKIQKAFLTMMQGRTSFIVAHRL
                     STIREADIILVMKDGKIIEQGNHESLLAANGFYANLYNSQFA"
     gene            complement(1313..1822)
                     /locus_tag="DHV81_00465"
     CDS             complement(1313..1822)
                     /locus_tag="DHV81_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93026.1"
                     /translation="MWISSYFIIREDDRRTGNLPCAPVAFHQNTKYITNKFPIILSLI
                     LYLPTAYQTPAPHTAGAHTSSPHFSHSVRDVSRRSDCPAAEALFRKRIAKLPSYCRIM
                     HTIHKNELPRMGAQGESRHGRRVSTVLSGIEALKSIIRTVSWQHSVANERTPLLQGSH
                     SGGLRPVFP"
     gene            complement(1823..2665)
                     /locus_tag="DHV81_00470"
                     /pseudo
     CDS             complement(1823..2665)
                     /locus_tag="DHV81_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743144.1"
                     /note="too many ambiguous residues; incomplete; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    2291..2381
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          688 a          563 c          640 g          683 t
ORIGIN      
        1 acaggaattg caacaatagc aggtacgctt attttcatgc tcacaataga tgtgaagata
       61 tcatgcattg tagtgctcat cacacctttg tcactttttg tggcaagctt tatcactaaa
      121 aagacttatt ccatgttcca gctgcagact agcacaagag gcgagcagac atcgctcatc
      181 gatgagatag taggaaatga aaaggtcgta caggccttca accatgagga tgaggcgctt
      241 gaacagtttg acgagataaa tgacagatta cagaaatgct cattgagggc aatatttttc
      301 tcaagtctta caaatccgtg tacacgtttt gtcaacagtc ttgtgtatgc cggagtcgga
      361 atttttggcg ctatgagtgc actcaccggc ggaatcacgg taggacagct ttcatgcttt
      421 ttatcatatg ccaaccagta caccaagccg ttcaacgaga tatcgggtgt cataacagag
      481 cttcagaacg cacttgcctg tgccgctcgt attttcgagc tgattgagga gaaaaaggag
      541 ataccggatg cttcggatgc ggtaacgctt ggtgaagcag acggacgtgt ggacatagag
      601 gatgtttatt tctcatacgt gccggataaa aagcttatcg aggatttcaa tctccacgta
      661 aagcctggac agcgcgtggc tatcgtaggt ccgacaggat gtggaaagac gactattatc
      721 aatctgctta tgagatttta tgatgtaaac agtggaacaa ttaaggtgag tggacatgat
      781 atcagaaaaa tcacgcgaga gagtctacgt gacaactatg gcatggtgct tcaggagaca
      841 tggctcaaga agggcacaat ccgtgacaat atcataatgg gaaagccgga tgccacagat
      901 gaggaaatca ttgcagcagc aaaggcatcc catgcccaca gctttatcag acgtcttccg
      961 aagggctatg acactgaaat cggtgaggac ggcggcagcc tgtcacaggg acagaaacag
     1021 cttttatgca tcactagagt catgctatgc ttaccgccta tgctgatatt ggatgaggct
     1081 acatcatcga ttgatacacg tacagagatt aagattcaaa aagcgttcct taccatgatg
     1141 cagggaagaa ccagctttat agtggctcat cgtctgtcca ccatcaggga agcggatatt
     1201 atccttgtta tgaaagacgg aaagattata gagcagggca atcatgagag tctgcttgca
     1261 gccaatggat tttatgcaaa tctctacaat agccagtttg catagcatag ggctatggaa
     1321 atacggggcg taagccgccg gagtgactgc cctgcagcag aggcgttctc tcgttcgcta
     1381 cgctatgctg ccaagatact gtacgaatta tgctttttag ggcttctatg cccgacaaaa
     1441 ccgtcgaaac gcgccgtcca tggcgcgatt ctccttgagc tcccatccgt gggagctcat
     1501 ttttgtgtat cgtgtgcata attctacagt agctaggcag cttcgctatg cgcttacgaa
     1561 agagcgcctc tgctgcaggg cagtcactcc ggcggcttac gtcccttacg ctgtggctga
     1621 aatgggggga ggatgtatgc gctcccgcgg tatgtggggc tggtgtctgg tatgctgtgg
     1681 gaagatacaa tataagggaa agtataattg gaaatttatt tgtaatatac ttagtatttt
     1741 gatggaaggc gaccggcgca cagggcaaat tccccgtgcg ccggtcgtct tcacgtatta
     1801 tgaaatatga agaaatccac atttactgcg cagcatcagg agctacatac gagtatgcat
     1861 tggacatctt tgcagcattg tttgtcagag tgacagcctc tctgtagtag gcaacaacag
     1921 gagtgccctt atccacagcg ttgaacagct gctccgcaaa agcaggtggc acattgatac
     1981 atccgtgtga gcctgaggtt ttgtaaattg ttcctccgaa ggtatcacgc cagctggcat
     2041 catggatacc tatattgtag gcaaacggca tgaaatagct gacaggagaa gagtatccct
     2101 cgccgacaag agtagcattt ctctgcttgt aaacaatctt gtatacacca tctacggaac
     2161 cattctgacg ggcgaggtta cctgatacga aatcagactc catctggaga gcaccatcct
     2221 tgtaatacca catgtgctgc tttgtgtagt caatctctat gtatgtatta cctatgtcgt
     2281 cagcaccgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcatttttc ttgctgacta
     2401 cccatccgta gtctccacca cctatttcaa tagtgtcacc ggacgatgtc ttgaatgagc
     2461 gtttgcggcc gaaggtattg tacttggaag caagctgctg gacatattta gtgaccttgg
     2521 catcatctat gctcacgctg ccgtcatctg agatgcttag catatcaagg attttggctg
     2581 agtcaagctt ctcgtcggca ccttcaattt tgtaagtgac ggttgaagca gtgtatttgt
     2641 cgagggcttc tttttcattt ttgat
//
LOCUS       DPUF01000017            4412 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_37727, whole
            genome shotgun sequence.
ACCESSION   DPUF01000017 DPUF01000000
VERSION     DPUF01000017.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_37727"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            120..>1096
                     /locus_tag="DHV81_00475"
     CDS             120..>1096
                     /locus_tag="DHV81_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase"
                     /protein_id="HCI93027.1"
                     /translation="MSSNNQSLAMQRIAKLVDENSFMEIGSLVTARSTDFNLTAAKAP
                     SDGVIIGHGLIDGNLVFIYSQDATVLNGTIGEMHAKKIASVYDMAMKMGAPVIGFIDC
                     GGIRLQESVDALDGFGLIYAKEVAASGVIPQICGVFGNCGGGLSVVPALCDFAYIEES
                     KGRMFVNTPDAIEGNRVEKCDTAAASFQSENNGCVDGIGSEDDIIADIRALVSMLPLN
                     NEGDVYTDSCEDDLNRACNSMDDMKADPRFLLSELSDDHVFFETKKDFAKNMVTGFVK
                     LNGMTVGAVANCSTVYDAEGKKAEEFALSLTAKGCNKAAEFVSFCDAFS"
     assembly_gap    1097..1245
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1246..1569
                     /locus_tag="DHV81_00480"
     CDS             <1246..1569
                     /locus_tag="DHV81_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase"
                     /protein_id="HCI93028.1"
                     /translation="EAYGSAYVAMNSKSIGADMVYAYPDAKIGMMDSKIAAEIMYPGA
                     DAKEIDEKAADYEKLQSSVSSAAARGYVDLIIDPADTRKYLVGAFEMLYTKYVDAPEK
                     KHGTK"
     gene            1589..2410
                     /locus_tag="DHV81_00485"
     CDS             1589..2410
                     /locus_tag="DHV81_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium pump decarboxylase subunit gamma"
                     /protein_id="HCI93029.1"
                     /translation="MKKKITYILSLLMIVCMLGACGSDNAEKTYGGYTTDELHTEATD
                     SLGNIQNYIALVDSCGGYDNYEKSVKEVEEKTGMTEGEVVYNTLGQSGASVSMKNIDA
                     ELDAVSSWLDLVDKYGDVKDATNGKDFTVTKSGGTLTTDMTLTFGKQDVTYELVYDSV
                     TMEVTGISLTPVQTMGQKLAKAGQNTVISMSIVFCVLILISLIIYCFKFIALIGNKPK
                     KAEAKSEPEKKETAAAAPAADLTDDTELVAVISAAIAASEGTTTEGFVVRSINRR"
     gene            2429..2791
                     /locus_tag="DHV81_00490"
     CDS             2429..2791
                     /locus_tag="DHV81_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCI93030.1"
                     /translation="MKNYTITVNGNVYDVTVEETGSTPSTASTPKRAAAPAAAAPKAA
                     APAGAGSIKVEAGAAGKVFKLEAKVGDAVKKGDTLLILEIMKMETPVVAPQDGTVASI
                     DVAVGDSVESGALLATLN"
     gene            2811..3959
                     /locus_tag="DHV81_00495"
     CDS             2811..3959
                     /locus_tag="DHV81_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HCI93031.1"
                     /translation="MSYVGETLSNLLHQTAFFNLSVGNYVMIAVACVFLFLAIKKGFE
                     PLLLVPIAFGMLLVNIYPDIIVSPEHSSNGVGGLLYYFYTLDEWSILPSLIFLGVGAM
                     TDFGPLIANPISFLLGAAAQFGIFGAYFLAILLGFNDKAAAAVSIIGGADGPTSIFLA
                     GKLGQTGLMGPIAVAAYSYMALVPIIQPPIMKLFTTKKERAIKMQNLRPVSKLERILF
                     PVVVTIVVCTLLPTTAPLVGMLMLGNLFRESGVVRQLQETASNALMYIVVILLGTSVG
                     ASTSAESFLNMSTIKIVILGLIAFMVGTAAGVLFGKLLCFFTKGKINPLIGSAGVSAV
                     PMAARVSQKVGAEEDPTNFLLMHAMGPNVAGVIGTAVAAGCFMAIFGI"
     gene            3982..>4412
                     /locus_tag="DHV81_00500"
     CDS             3982..>4412
                     /locus_tag="DHV81_00500"
                     /EC_number="4.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882851.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HCI93032.1"
                     /translation="MSEVVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVG
                     YNAVECWGGATFDACLRFLKEDPWDRLRKLRDGFKNTKLQMLFRGQNILGYRPYADDV
                     VEYFVQKSIANGIDIIRIFDCLNDIRNLQTAVTACNKEKGHA"
BASE COUNT         1263 a          810 c          996 g         1194 t
ORIGIN      
        1 aattttttct aaacaaaaat taaagttaaa aatttaacaa attagttgaa aaaaaattag
       61 aaatagtcta ttatcatact agcgaaaaat tttatttttt tatagatgga ggatataaaa
      121 tgagtagtaa caatcaaagc ttggcaatgc agcgtattgc taagttagtc gacgagaaca
      181 gtttcatgga aattggttct cttgtgacag ctcgcagtac agattttaac cttactgcag
      241 caaaagctcc ttcagatggt gttattatcg gacacggtct gattgatgga aatcttgtat
      301 ttatctacag ccaggatgca acagttctta acggaacaat cggcgagatg catgcaaaga
      361 aaattgcttc tgtatatgat atggccatga aaatgggcgc acctgttatc ggatttatcg
      421 attgcggtgg tatcagactt caggagtcag ttgatgcatt agacggattt ggtcttatct
      481 acgccaagga agttgcagca agtggcgtta ttccacagat ttgtggtgta ttcggtaatt
      541 gtggtggcgg actttctgta gtaccggctc tttgtgattt cgcttatatc gaagagtcta
      601 agggcagaat gtttgttaat actcctgatg caatagaggg taatcgcgtt gagaaatgcg
      661 atacagcagc agcttcattc cagagtgaga acaacggatg tgtagacgga atcggttcag
      721 aggatgatat tatcgctgat atcagagctc ttgtcagcat gcttccactc aacaacgagg
      781 gagatgtata tacagactca tgtgaggatg atctcaacag agcatgtaac agcatggatg
      841 atatgaaggc tgatccaaga ttcctcttat cagagctttc tgatgatcat gtattctttg
      901 agacaaagaa agactttgcc aagaatatgg taacaggctt tgttaaatta aacggcatga
      961 cagtcggtgc agttgcaaac tgcagcacag tatatgatgc tgagggcaag aaggcagagg
     1021 agtttgcact ttctcttact gcaaagggct gcaacaaggc agctgagttt gtatcattct
     1081 gtgatgcttt ctcaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaggc ttacggaagc
     1261 gcatatgttg caatgaactc taagtcaatc ggagctgata tggtttatgc ataccctgat
     1321 gcaaagattg gtatgatgga ttcaaagatt gcagctgaga ttatgtatcc gggcgctgat
     1381 gcaaaggaaa tcgatgagaa ggcagctgat tacgagaagc ttcagtcttc tgtttcatca
     1441 gcagcagcca gaggatatgt agatctcatc attgatccgg ctgacacaag aaaatatctt
     1501 gtaggtgctt ttgagatgct ttacaccaaa tatgtagatg caccagagaa gaaacacgga
     1561 actaaataat agaaaggcgc attttgttat gaagaaaaaa ataacatata tccttagcct
     1621 tcttatgatt gtatgtatgc ttggagcatg tggctctgac aatgccgaaa agacatacgg
     1681 cggatacaca acagacgagc ttcatactga ggcaacagac agccttggca atatccagaa
     1741 ctacattgcg ctcgtagata gttgtggcgg ttatgacaac tatgagaaga gtgtcaagga
     1801 agttgaggag aagacaggca tgacagaggg tgaggttgtt tacaacacac tcggacagtc
     1861 gggagcaagc gtttcaatga aaaatatcga cgctgagctt gatgccgttt catcatggct
     1921 tgatctggtt gacaaatacg gagacgtaaa ggatgcaact aatggcaagg attttacagt
     1981 aaccaagtca ggtggtacac tcactaccga tatgacactt acatttggca agcaggatgt
     2041 aacatacgaa ttagtatatg attcggttac catggaggta acaggtatct cacttactcc
     2101 tgttcagaca atggggcaga agcttgctaa ggcaggacag aacacagtaa tttcaatgag
     2161 tattgtattc tgtgtactta ttcttatctc tcttatcatt tactgcttca agtttattgc
     2221 tcttatcggc aataagccaa agaaggcaga agcaaagagc gagcctgaaa agaaagagac
     2281 tgctgcagct gcacctgctg cagaccttac agatgataca gagcttgtag ctgttatctc
     2341 agcagctata gcagcaagcg agggtactac aacagagggc tttgtagtcc gttcaatcaa
     2401 tagaagataa tttaggagga catttgagat gaaaaactat acaattaccg ttaacggaaa
     2461 tgtttacgat gttacagtag aagagacagg aagcacacct agcacagcaa gcacacctaa
     2521 aagagctgca gcacctgctg ccgctgcacc aaaggctgct gcacctgcag gagcaggaag
     2581 catcaaggtt gaggccggag ctgccggaaa ggtatttaag ctcgaggcta aggttggcga
     2641 tgcagtaaag aagggtgata cacttcttat tcttgagatc atgaagatgg agacacctgt
     2701 tgttgcacca caggatggaa cagtagcaag cattgatgtt gctgttggtg attcagtaga
     2761 gtcaggtgca ttacttgcaa cattaaacta attccaggag gtaaactaat ttgagttacg
     2821 taggtgaaac actttcaaac ctcctacatc agactgcctt ttttaatctg tcagtcggaa
     2881 actatgttat gattgccgta gcatgtgtat tcctgttttt ggctattaaa aaaggatttg
     2941 agcctttact tttagtacct attgcttttg gtatgctcct tgttaatatc tatcctgata
     3001 ttattgtaag cccggagcac tcaagcaatg gagtaggtgg tttactttat tatttctata
     3061 cacttgatga gtggtcaatt cttccttcac tgattttcct cggtgtcgga gctatgacag
     3121 actttggtcc gcttatcgca aaccctatca gcttcttact cggagctgca gcacagtttg
     3181 gtattttcgg agcttatttc cttgcaatcc ttctcggatt taatgacaag gcagcagcag
     3241 ccgtatctat catcggtgga gctgacggac ctacatctat tttccttgcc ggaaaattag
     3301 gtcagacagg tctgatggga cctatcgccg tggcagcgta ctcgtacatg gcattagtac
     3361 ctatcatcca gccgccaatt atgaaattat ttactacaaa gaaagagcgt gcgatcaaga
     3421 tgcagaacct tcgtcctgta agcaagcttg agagaattct cttccctgta gtagttacta
     3481 tcgttgtttg tacactgctt ccaactacag caccactcgt tggtatgctc atgcttggaa
     3541 accttttccg tgagtctggt gtagtacgtc agcttcagga gacagcaagc aacgccctta
     3601 tgtacattgt agttatctta ctcggtacat cagtaggtgc ttctacaagc gcagagtcat
     3661 tccttaatat gagcacaatc aagatcgtta tccttggtct tattgcattc atggtgggaa
     3721 ctgctgccgg agtacttttt ggaaagcttt tatgcttctt tacaaaaggc aagatcaatc
     3781 cacttatcgg ttcagccggt gtatccgctg ttcctatggc tgcccgtgta tctcagaagg
     3841 tcggtgcaga agaggatcca acaaacttcc ttcttatgca tgctatggga cctaacgtag
     3901 ccggtgttat tggaactgcc gtagcggccg gatgcttcat ggctatattc ggtatttaga
     3961 tttaattaag gaggattaat catgtcagaa gtggtaaaga aaccattaaa aataacagaa
     4021 actgttcttc gtgatgcaca tcagtcactg attgcaacca gaatgacaac agagcagatg
     4081 cttccaatcg ttgacaaaat ggacaaggtt ggatacaacg cagtagagtg ctggggtggt
     4141 gcaacatttg atgcctgcct tagattctta aaggaagatc catgggatcg tctcagaaaa
     4201 ttaagagacg gcttcaaaaa tacaaagctc cagatgcttt tccgtggaca gaatatctta
     4261 ggatatcgtc catatgcaga tgatgttgtt gagtatttcg tacagaagtc aatcgcaaac
     4321 ggaatcgata tcatccgtat tttcgactgc ttaaatgata tcagaaactt acagacagca
     4381 gttacagcat gtaacaagga aaagggacat gc
//
LOCUS       DPUF01000018           19095 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10962, whole
            genome shotgun sequence.
ACCESSION   DPUF01000018 DPUF01000000
VERSION     DPUF01000018.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 19095)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19095)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19095
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10962"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            43..1014
                     /locus_tag="DHV81_00505"
     CDS             43..1014
                     /locus_tag="DHV81_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93033.1"
                     /translation="MTLYRVADKSQIIDALIEAAENGKEVIVLVELRARFDEANNIEM
                     SHRLEDAGCQILYGLGDYKVHSKLCLITKKKGDGYEYITQIGTGNYNEKTSRLYTDLS
                     LITANQEIGADASRVFMALQRGETVSDGDVKHLLVAPKCLQNRIVDMIDEQIAKKNAG
                     KPAYIGVKINSLTDKVLIDKLIDASQAGVKVELIVRGICCVKPQVEGATENITVISVV
                     GRYLEHSRIYRFGVGDDEKIYIASADFMTRNTVRRVEVATPVYDANAKDKIRHIFDTI
                     MTDDEKGKELRADGEYVDRKINSTPIDSQEQFFDEAYKNADKSADNQ"
     gene            1383..2276
                     /locus_tag="DHV81_00510"
     CDS             1383..2276
                     /locus_tag="DHV81_00510"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01380.20,HMM:PF01418.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCI93034.1"
                     /translation="MWKGKLMNEKENIDYKIRTMYSEMRKSEKKVADYVLKNELECGR
                     ISLEKLAKRSDVSQPTVVRFVKALGYDSFKEFRYSLVREISELESRRMKDYSLQNYSI
                     NPGDDLKYVPAKVIASTVGILENMLKSISQDEFKKAIRMIAKAKKIVICAVENSTAVA
                     NDLNTKLLYLGYDCRFDEDYYIQHIQVAAMTENDLVIGISYSGQSKDTVDAMKLAKKS
                     KAQTICITNFAESRISRWGDVVLCSSQKQWMYGDCIFSRSSQMVINDMIYMGLITFDY
                     NRCTENLDKNSEIIQNRGYDL"
     gene            2423..3193
                     /locus_tag="DHV81_00515"
     CDS             2423..3193
                     /locus_tag="DHV81_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93035.1"
                     /translation="MLELEHIKKAYDGVTILNDINLQINDGEIVSILGPSGCGKTTLL
                     NLILGLVNADGGKIIFNGNDITDTPMEKRGFNIVFQDYALFPNLNVIKNIRYGLENKP
                     GISSEKEINDLIELLGLKEHLNKRIEQLSGGQKQRVALARTLVMKPKILLLDEPLSAL
                     DGVIKESIKAKIREIAREYHLTTVIVTHDPEEALTLSNHVMIINKGSISQFGTPDEIV
                     NTPKNDFVEKFILQQLEIKRNNIYTLFESPELITGVGA"
     gene            3202..5853
                     /locus_tag="DHV81_00520"
     CDS             3202..5853
                     /locus_tag="DHV81_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCI93036.1"
                     /translation="MVKRKSKEIKIIIALITILFAAFLAYPAARLLVKSFVNDSGLTL
                     SMYKEVFAGKGFLKALLNSFKVAGASGIVTTFIAFCMAYTVHYTNSPAIVKRLIKIIA
                     ALPMLLPTLTYGFAIIYSFGKQGLLTRLFGRQLFNIYGFNGLLIGYVIYTLPVAFMLI
                     NNTLGYVDKKFTVVSRLMGDSAFKNFMMTVIRPLLGTFATAFIQCFFLCFTDFGIPAS
                     VGGKYEVIASVLYRQMLGSVPDFGKGSVVAIIMLVPSVVSIIILTYLERYNIRYNRVS
                     SVELKKSYVRDTVCSILSVLFCVCVVSIFAVIFIIPFVQDWPYKTSFSIVHFKTALLN
                     EGLSEVYKNSIIVAILTAVIGTAMAYAAALVTARSNAAAKSKKVIDYIAMVTNTIPGM
                     VLGLAFLFTFSGTFLQGTFAIIIICNIIHYFSTPYLMIKGSLEKMNASWETTAMLMGD
                     NWIKTIFRVVTPNAASTLIEVFGYYFVNAMVTISAVIFIAGARTMVLTTKIKELQYYN
                     KFNEVFVLSLLILATNLTAKLVIGLLSDVNKVKEFKEKIKMKKGILRRAACAGIVGVV
                     GLSTLFVGGCGSKKSEDSQVVIYSNADDEAVAAMKDALDSNGFKNQYIFQTFGTSELG
                     GKLLAEGKNIEADLVTMSTFYVESAQEQNKMFKALDFEHNTLEEYPSYCTPITAQEGT
                     IIVNTELMKENKLDNPTSLKDLAKPEYKGQIAVTDIASSSTAWLLIQGLVSEYGEDGA
                     KDVLKGIYENAGDHIEESGSGPLKLVRAGEAAVGFGLRQQAVADKESGLPIDYIDPKE
                     GNFTLTESAAVVDKGDKTNSKAMEMAKCIIEKGRAKLLESYPIPLYDGETVADSEKSG
                     NPKKFSEPLTVDLLKKHQALSEECKPQ"
     gene            5912..7000
                     /gene="phnW"
                     /locus_tag="DHV81_00525"
     CDS             5912..7000
                     /gene="phnW"
                     /locus_tag="DHV81_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCI93037.1"
                     /translation="MKNYKLLTPGPLTTTDTVKKEMLFDHCTWDDDYKAITQEIRSKL
                     LKLAHVSEDEYTAVLMQGSGTFGVEAVITGVIGEGEKLLICANGAYGERMEDIAKHAH
                     ISYTVYHEDYDKVPDAVKVGKILDSDPDITHVSMVHSETTSGILNDIESVSRVVKAHR
                     RTMIVDAMSSFGGVDIPVADWGIDFIISSANKCIQGVPGFSFIIANKEKLIASKGKAT
                     SLSLDIYDQWETMSKDGKWRFTSPTHVVLAFAQALRELEQEGGITARHKRYSDNNKLL
                     ISRMRELGFKTYIDEAHQGPIITTFLYPENRKFDFADMYEYIKERGYAIYPGKVTEAD
                     TFRIGNIGEIYEEDIIKVTDIIRDFLAE"
     gene            7034..7810
                     /locus_tag="DHV81_00530"
     CDS             7034..7810
                     /locus_tag="DHV81_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCI93038.1"
                     /translation="MKQENRFDAVIFDWAGTTVDYGCFAPVQAFIEVFKHFGIEPTME
                     EVRKPMGMLKRDHIKTMLSMDRISNEWQKKYGRIWKEEDVDALYALFEEKLMGILDGF
                     ADVKPYVLETVDELRKRGIMIGSTTGYTDKMMEIVVSKAKENGYEPDAWFSPDSTGNF
                     GRPYPYMIFKNMQRLGVMSVERVMKVGDTVSDIREGRNAGVYTVGIVEGSSELGLTKE
                     EYDKMGDIERIARVEAVKERFLKAGADEVVLNMRSLMAIM"
     gene            8110..8589
                     /gene="ybaK"
                     /locus_tag="DHV81_00535"
     CDS             8110..8589
                     /gene="ybaK"
                     /locus_tag="DHV81_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCI93039.1"
                     /translation="MGKKEAKTNAIRILETMKIPYEARTYECDDFVDAAQIADKLGLD
                     HAGMYKTITTVGKSGGYYVFVVPIDDEIDFKKAAKAAGEKSMEMLHLKDLTKVTGYIR
                     GGCTSIGMKKQYPTFIHEAAKEQDKITVSGGRLGLQITLSPDDLCRAAKAEYADIIK"
     assembly_gap    8644..8701
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8734..9833
                     /gene="trpS"
                     /locus_tag="DHV81_00540"
                     /pseudo
     CDS             8734..9833
                     /gene="trpS"
                     /locus_tag="DHV81_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741323.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
     assembly_gap    9088..9173
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9919..10880
                     /locus_tag="DHV81_00545"
                     /pseudo
     CDS             9919..10880
                     /locus_tag="DHV81_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515533.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
     assembly_gap    10038..10121
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10961..11848)
                     /locus_tag="DHV81_00550"
     CDS             complement(10961..11848)
                     /locus_tag="DHV81_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HCI93040.1"
                     /translation="MWLADRWKDYEVIDCSCGEKLERWSKYILLRPDPQVIWDTPKTN
                     KNWKKLNGHYHRSKKGGGEWEFFDLPQEWTINYSLPINKELTFHLKPFSFKHTGLFPE
                     QAANWDWFSKKIYDAKKSGRDVKVLNLFAYTGGATLAAAAAGAAVTHVDASKGMVTWA
                     KENAASSGLGDAPIRWLVDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEE
                     SVYPLVQLCAKLLTDKPLFFLINSYTTGLQPAVLTYMISTALKKFDGKVTASEIGLPV
                     SSNGLVLPCGASGRFESIC"
     gene            12265..12582
                     /locus_tag="DHV81_00555"
     CDS             12265..12582
                     /locus_tag="DHV81_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCI93041.1"
                     /translation="MASQVMRITLKAYDHELVDSSAKKIIETVKKNGSQVSGPVPLPT
                     KKEVVTILRATHKYKDSREQFEQRTHKRLIDIINPTQKTTESLSRIEMPAGVYIDIKM
                     KQK"
     gene            12761..13444
                     /locus_tag="DHV81_00560"
     CDS             12761..13444
                     /locus_tag="DHV81_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCI93042.1"
                     /translation="MKKAILATKIGMTQIFNADGVLVPVTVLEAGPCVVTQIKTVEND
                     GYSAVQVGFGDKKEKVVEKDANGKKSVAHRHGVNKAQMGHFKKAGVSGKRFVREFKFE
                     NADEYNLADEIKADIFAEGDKVDVTAISKGKGFQGAIKRLGQHRGPMAHGSKFHRHQG
                     SNGACSSPSKVFKGKGMPGHMGSVKVTTQNLEVVRVDADKNLLLIKGAVPGAKKALIT
                     VKETTKSGK"
     gene            13473..14093
                     /locus_tag="DHV81_00565"
     CDS             13473..14093
                     /locus_tag="DHV81_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCI93043.1"
                     /translation="MANVAVYNMEGKEVDKIELNDSIFGVEINEHLVHMAVLQQLANK
                     RQGTQKAKTRSEVRGGGRKPWRQKGTGHARQGSTRSPQWTGGGVVFAPTPRDYSFKLN
                     KKEKRAALKSALTSRVVENKFVVVDELKLDEIKTKKFVEVLKNLNVEKALVVLNDMDE
                     KVIASAANIPTVKTTQTNELNVFDVLKYDTVVVTKAAVATIEEVYA"
     gene            14093..14392
                     /locus_tag="DHV81_00570"
     CDS             14093..14392
                     /locus_tag="DHV81_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCI93044.1"
                     /translation="MANVQYYDVILKPVVTEKSMNAMAEKKYTFLVHPDANKTMIKEA
                     VERMFEGTKVAKVNTMNCEGKNKRRGMVTGKTAKTKKAIVQLTADSKDIEIFAGL"
     gene            14472..15317
                     /locus_tag="DHV81_00575"
     CDS             14472..15317
                     /locus_tag="DHV81_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCI93045.1"
                     /translation="MGIKTYNPYTPSRRNMTGSDFSEITKTTPEKSLVTSLKKNAGRN
                     NQGKITVRHQGGGNRRKYRLIDFKRNKKDGIPATVIGIEYDPNRTANIALICYADGEK
                     SYILAPAGLTDGMKVMSGAQAEVRVGNCLPLENIPVGTQIHNIELLPGKGGQLVRSAG
                     LSAQLMAKEGKYATLRLPSGEMRMVPIQCRATIGVIGNGDHNLVNIGKAGRKRHMGVR
                     PTVRGSVMNPNDHPHGGGEGKAPVGRPGPCTPWGKPALGLKTRKKNKQSNKMIVRRRD
                     GRAIK"
     gene            15334..15615
                     /locus_tag="DHV81_00580"
     CDS             15334..15615
                     /locus_tag="DHV81_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCI93046.1"
                     /translation="MARSLKKGPFADESLLKKVDAMNASGDKSVIKTWSRRSTIFPSF
                     VGHTIAVHDGRKHVPVYVTEDMVGHKLGEFVATRTYRGHGKDEKKSGVR"
     gene            15641..16036
                     /locus_tag="DHV81_00585"
     CDS             15641..16036
                     /locus_tag="DHV81_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCI93047.1"
                     /translation="MAKGHRSKIKRERNEVRDTRPSAKLSYARVSVQKACFVLDAIRG
                     KSVNEALAIVTYNPRYASSLVKKLLESAIANAENNYPEKNYKAENLYVAECFANKGPT
                     MKRIHPRAQGRAYRIEKRMSHITIVLDER"
     gene            16046..16702
                     /locus_tag="DHV81_00590"
     CDS             16046..16702
                     /locus_tag="DHV81_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCI93048.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSKWYAEGDFADYLVEDYNIRTFLKK
                     KLYAAGVSKIEIERASDRVKVIIYTAKPGVVIGKGGAEIEKIKAEVQKLTDKKLVVDI
                     KEVKRPDKDAQLVAENIALQLENRVSFRRAMKSCMGRAMKAGVKGIKTTCSGRLGGAD
                     MARTETYNDGTTPLQTIRADIDYGFAEADTTYGKVGVKVWIYKGEVLPTKANKEGGAK
                     "
     gene            16705..17142
                     /locus_tag="DHV81_00595"
     CDS             16705..17142
                     /locus_tag="DHV81_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCI93049.1"
                     /translation="MLMPKRVKHRKQFRGSMAGKATRGNKITNGEYGIVALEPCWIKA
                     NQIEAARVAMTRYIKRGGKVWIKIFPEKPVTHKPMGVRMGKGKGALEYWVAVVKPGRV
                     LFEISGVPEDVAKEALRLATHKLPCKCKVVSRADLEGGESNEN"
     gene            17132..17335
                     /locus_tag="DHV81_00600"
     CDS             17132..17335
                     /locus_tag="DHV81_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCI93050.1"
                     /translation="MKTSAYVKELQAQSVEQLQAQLVDAKKELFNLRFQNATNQLDNT
                     ARIKEVRKNIARIQTVITAKAAE"
     gene            17366..17623
                     /locus_tag="DHV81_00605"
     CDS             17366..17623
                     /locus_tag="DHV81_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCI93051.1"
                     /translation="MEERNLRKTRVGVVVSDKMDKTIVVAVRDNVRHPLYNKIVKKTY
                     KLKAHDEKNDAKIGDTVRVMETRPLSKDKRWRLVEIMERAK"
     gene            17642..18010
                     /locus_tag="DHV81_00610"
     CDS             17642..18010
                     /locus_tag="DHV81_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCI93052.1"
                     /translation="MVQQESRLKVADNTGAKELLVIRVMGGSTRRYANIGDVIVATVK
                     DATPGGVVKKGDVVKAVVVRSVKGARRKDGSYIKFDENAAVIIKDDKTPKGTRIFGPV
                     ARELREKQFMKIVSLAPEVL"
     gene            18022..18333
                     /locus_tag="DHV81_00615"
     CDS             18022..18333
                     /locus_tag="DHV81_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCI93053.1"
                     /translation="MATMKIKKGDLVKVIAGKDINNEGKVIAVNAKNNTLLVEGINMV
                     TKHTKPSMSNQQGGIVHQEAPIDASNVMLIHDGKPTRVGFKMDGDKKVRFAKSTGKVI
                     D"
     gene            18357..18896
                     /locus_tag="DHV81_00620"
     CDS             18357..18896
                     /locus_tag="DHV81_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCI93054.1"
                     /translation="MSRLREQYENEIKDAMIKKFGYKNAMEIPKLDKIVVNMGVGEAK
                     ENAKVLEAAVKDMEAITGQKAVTTKAKNAIANFKIREGMPIGCKVTLRGEKMYEFADR
                     LINLALPRVRDFRGVNPNAFDGRGNYALGIKEQIIFPEIEYDKVDKVRGMDIIFVTTA
                     KTDEEARELLAQFNMPFAK"
     gene            18911..>19095
                     /locus_tag="DHV81_00625"
     CDS             18911..>19095
                     /locus_tag="DHV81_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCI93055.1"
                     /translation="MAKTSMKIKQQRAPKFSTRAYTRCRICGRPHSVLKKYGICRVCF
                     RELAYKGQIPGVKKASW"
BASE COUNT         6319 a         3208 c         4534 g         4806 t
ORIGIN      
        1 cttaacgatg cagcagagga tcctgaagta gtttccatca agatgacact atacagagtg
       61 gcagacaaga gccagattat cgatgcactt atagaggctg cagaaaacgg caaggaggtt
      121 atcgtgcttg tcgagctgcg tgcgaggttt gatgaggcta acaacataga gatgtcacac
      181 cgccttgagg atgcaggctg tcagatactt tacggactcg gcgattacaa ggttcactca
      241 aagctctgcc ttatcacaaa gaaaaagggc gatggctatg agtacatcac tcagatcggt
      301 acaggaaact acaatgagaa gacatcgcgt ctttacacag atttgtccct catcactgca
      361 aatcaggaga ttggagcgga tgcatcaagg gtatttatgg cgcttcagag aggagagact
      421 gtttcggacg gcgatgtaaa gcatctgctt gttgcaccaa aatgcttaca gaacaggatt
      481 gttgacatga ttgatgagca gattgccaag aagaatgcag gaaaacctgc atatatcgga
      541 gtaaaaatta actctctcac tgacaaggtg cttatcgaca agcttatcga tgcatcacag
      601 gcaggagtga aggtagagct tattgtcaga ggaatctgct gcgtgaagcc acaggttgag
      661 ggcgctacag aaaatatcac ggtaataagc gttgtaggaa gatatcttga gcattcacgt
      721 atatacagat ttggtgtggg cgatgatgag aagatttaca ttgcatcagc agattttatg
      781 accagaaaca ccgtaagacg tgtggaggtt gcaacacctg tatacgatgc aaatgctaag
      841 gacaaaatcc gccatatctt tgatacgatc atgacagatg atgagaaggg caaggagctt
      901 cgcgctgatg gcgagtatgt ggacagaaag ataaacagca caccgataga ttcgcaggag
      961 cagttttttg atgaagcata caaaaatgcc gataagagtg cggacaatca gtaaaatgag
     1021 ataattgcat gaaggctgct acatacgtgt aaaagtgtat gtagcagcct ttattgttta
     1081 cgatattact agatgtcaga ccgactgtta taggaccgat attctatgat gttcgatgcc
     1141 gcacataaaa ctatgactgc aagtattttt ccggcgcttt aagcaggtaa aaaacctgga
     1201 caaaaagata gaatataata gactttttaa cacgatttac atatgtttta cacaataaaa
     1261 ttcgttttta tgtagaatat aaaataagaa atatgtagtt atactacaaa cattatttat
     1321 aaaatttgta gtataactac aagcctgaga atgtacaaga acagttaccg gaaatatata
     1381 atatgtggaa aggaaaatta atgaacgaaa aagaaaatat agattataaa atcaggacaa
     1441 tgtattcaga aatgcggaaa tcagagaaga aggtggcaga ctatgtactc aaaaatgagc
     1501 ttgaatgtgg aaggatttcc cttgaaaaac tggcaaagag gagtgatgtg agccagccta
     1561 cggtggtacg attcgtaaag gcacttggat atgacagctt taaggagttc cgctacagcc
     1621 ttgtcaggga aatatcagag cttgagagca gaagaatgaa ggattattca ctgcaaaact
     1681 acagtataaa tccgggggat gatttaaagt atgtgccggc gaaggtgatt gcatcgacag
     1741 tcggaatatt ggagaatatg ttaaagagca tatcgcagga tgagtttaaa aaggcaataa
     1801 ggatgatcgc aaaggcaaaa aagattgtga tatgtgctgt agaaaattca acagcagtgg
     1861 caaatgattt gaacacaaag ctcttatacc tggggtatga ttgtcggttt gatgaggact
     1921 attatataca gcatattcag gttgctgcaa tgacagagaa tgatctggtg ataggtatat
     1981 catattccgg tcagtcaaag gataccgtgg atgcgatgaa gctggcaaag aaatcaaaag
     2041 cgcagacaat ttgtattaca aattttgccg agtcgagaat atccagatgg ggtgatgtgg
     2101 tgctgtgcag ttcacagaag caatggatgt atggcgactg tattttctca agaagttctc
     2161 agatggttat aaacgatatg atatacatgg ggcttatcac attcgattat aaccggtgca
     2221 cggaaaacct ggacaagaac agtgaaataa tacaaaacag aggatatgac ctgtagtttt
     2281 tctacaacag gtcatatcct tttttgttac atgtgtttac gagttcgtta caaaagcctt
     2341 actgggattt gattgtattt aatgtgtggt aaatctatac tcaagtcagc ttgaaggaaa
     2401 gcaacagaaa aaggagataa atatgcttga gttagaacat atcaaaaagg catatgacgg
     2461 cgtaacaatc ttaaatgaca taaatttaca gataaatgat ggtgagattg tttccatact
     2521 cggaccgtca ggatgcggta agacaacatt attaaatctt atattgggat tggttaatgc
     2581 agatggaggt aaaattattt tcaacggaaa tgacatcaca gacactccga tggaaaagag
     2641 aggctttaat atagtatttc aggattacgc tcttttccca aacttaaatg ttattaaaaa
     2701 tatcaggtac ggcttggaaa ataaacccgg tatttcatct gaaaaggaaa tcaatgattt
     2761 gatagagctg cttgggttaa aggagcatct gaataagagg attgaacagc tttcaggagg
     2821 acagaagcag agagtggcac ttgcaagaac tcttgtaatg aagcctaaaa tattattgct
     2881 ggatgagcca cttagtgccc ttgatggtgt tataaaagaa tcaatcaaag caaaaatcag
     2941 ggagatagca agagagtatc atcttacaac agttattgtc acacatgatc cggaggaggc
     3001 acttacgctt tcaaatcatg tcatgataat aaataaggga agcatctcac agtttggaac
     3061 cccggacgaa atagtgaata caccgaaaaa tgattttgtt gagaaattta ttttgcagca
     3121 gcttgagatt aaacgaaaca atatctatac attatttgag tcacccgagc tgataacagg
     3181 tgttggagca tagaggtgtc aatggtgaaa agaaaaagta aagaaataaa gataatcatt
     3241 gcattgataa caatactttt tgcagcgttt ttagcctatc cggctgcaag acttctggtt
     3301 aaatcctttg tcaatgacag cggactgaca ttgagtatgt ataaggaagt atttgcagga
     3361 aaagggtttc ttaaagcatt attaaacagc tttaaggtag ccggcgcaag tggaattgtt
     3421 actacgttta ttgcattttg catggcttat acagttcatt atacaaactc accggccata
     3481 gttaaaaggc ttataaaaat aatcgcagca ctgccaatgc ttcttccgac actgacctat
     3541 ggatttgcaa tcatctattc attcggaaaa caaggacttt taacaagact ttttggcaga
     3601 cagcttttta acatatatgg attcaatggg cttttgattg gatatgtaat atacacactt
     3661 ccggttgcgt ttatgctcat aaacaataca ttgggatatg ttgacaaaaa attcactgta
     3721 gtgtcacgtc tgatgggcga tagtgcgttt aagaatttta tgatgacagt aatcagacca
     3781 ttgcttggca catttgcaac cgcatttata cagtgttttt tcctttgctt cacagatttt
     3841 ggtattccgg catctgttgg gggcaaatac gaggtcattg caagcgtact ctacaggcag
     3901 atgcttggaa gcgtgcctga ttttgggaag ggcagtgtgg tagccattat catgcttgtg
     3961 ccgtcagtag tgagcattat tattctgaca taccttgaaa ggtacaatat cagatataac
     4021 agggtgtcat cagtggagct taaaaagagc tatgtaaggg atacagtatg cagtatatta
     4081 agcgttttgt tctgcgtatg tgtggtatca atttttgcag taatatttat cattccgttt
     4141 gtacaggact ggccgtataa aacatcattt tcaatagttc attttaaaac agcattgctt
     4201 aatgaaggac tttcagaggt atacaaaaac tctattatag ttgcgatatt gactgctgtt
     4261 atcggaacgg ctatggcata tgcagctgca cttgtgacag cgcggagcaa tgcagcggct
     4321 aaatcaaaaa aggtgattga ttatatagcg atggttacaa acacaatacc gggaatggtt
     4381 ctgggactgg catttttgtt cacattttca ggaacatttt tgcagggaac atttgcaata
     4441 atcatcatat gtaacataat acattatttt tcaacaccat atcttatgat taagggctcg
     4501 cttgagaaga tgaatgcttc atgggagaca acggcgatgc tgatgggaga taactggatt
     4561 aagacaattt tcagggttgt tacaccaaat gcagcatcaa cgctaataga ggtgtttgga
     4621 tactactttg taaatgcaat ggttactata agcgcggtta tttttatcgc aggtgcaaga
     4681 accatggtgc ttacgacgaa gataaaagag cttcagtatt ataacaagtt caatgaagta
     4741 ttcgtgcttt cgttgcttat acttgctacc aatctgactg caaagcttgt gattggcctg
     4801 ctgtcagatg taaataaagt caaagaattt aaggagaaaa ttaaaatgaa aaaaggaata
     4861 ttaagaagag ctgcatgcgc aggaattgta ggtgtggtag ggctgtcaac attgtttgtc
     4921 ggtggttgtg gcagcaaaaa aagtgaagat tcgcaggtgg ttatatattc aaatgctgat
     4981 gatgaggcag tggctgcaat gaaggatgca cttgattcaa atggcttcaa gaaccagtat
     5041 attttccaaa ccttcggaac atcggagctt ggaggaaagc ttttggcaga gggcaaaaac
     5101 atagaggctg accttgttac catgagtaca ttttatgtgg agagtgcaca ggaacagaac
     5161 aagatgttta aggctcttga ttttgaacac aatacacttg aggaatatcc ttcgtattgc
     5221 acacctataa ctgcgcagga aggtacaatc atcgtaaata cagagctgat gaaggaaaac
     5281 aagcttgaca atccgacatc acttaaggat cttgcaaagc ctgagtataa gggacagatt
     5341 gcagtgacag atatcgcatc atcatctacg gcatggcttt taattcaagg gcttgtatca
     5401 gaatatggtg aggatggcgc aaaggatgta ttaaagggaa tatatgagaa tgcgggtgac
     5461 catatagagg aatccggttc gggaccactt aagcttgtca gagcaggtga ggctgctgtg
     5521 ggctttggcc tcagacagca ggcggtggca gataaggagt caggtcttcc gattgattat
     5581 atagatccga aagaaggcaa ctttactctc acagaatcag cagcagttgt agataagggt
     5641 gataagacaa attctaaggc gatggagatg gcaaaatgca tcattgaaaa gggcagggca
     5701 aagcttttag agagttatcc aattccgtta tatgatggtg agacagttgc agattcggaa
     5761 aaatcaggca acccaaagaa attctcagag ccgctcacag ttgatttgtt aaagaagcat
     5821 caggctcttt cggaggagtg caagccacag taggctgata aatgaaaata tcaaataaat
     5881 atattattac agaaggaaga ttgaattgat aatgaaaaac tataaattac ttacaccggg
     5941 accacttacc acaacagata cagtaaaaaa ggagatgctt tttgaccatt gcacatggga
     6001 tgatgattac aaagccatca cgcaggaaat tcgctcaaag cttttaaagc tggcacatgt
     6061 gtcagaggat gagtacacag cggtacttat gcagggaagc ggaacctttg gtgtggaggc
     6121 tgttataacc ggtgtaattg gcgaaggtga gaagctttta atctgtgcaa acggagccta
     6181 tggcgaaaga atggaggata ttgcaaagca tgcacatatt tcttataccg tatatcatga
     6241 ggattatgac aaagtacctg atgcagtaaa ggttggtaaa attttggaca gtgaccctga
     6301 tatcacacat gtctcaatgg ttcacagtga gacaacctca ggtattttaa atgatattga
     6361 gtcagtaagc cgtgtagtaa aggcgcatag gaggactatg attgtagatg caatgagcag
     6421 ctttggaggc gtggatatac ctgtagcaga ctggggcatt gattttatca ttagcagtgc
     6481 caacaagtgt atccagggtg ttccggggtt ttcgtttatc attgccaata aagagaagct
     6541 tatcgcctca aagggaaaag ccacaagtct ctcattagat atctacgacc aatgggagac
     6601 aatgagcaag gacggaaaat ggagatttac gtcacctaca catgttgtgc ttgcatttgc
     6661 ccaagcgctt cgagagctgg aacaagaggg aggaattaca gcaagacata agagatattc
     6721 ggacaacaat aagcttctta tcagtagaat gagagaactt ggatttaaaa catatattga
     6781 tgaggcacac cagggaccga ttatcacaac ctttttatat cctgaaaacc gtaaatttga
     6841 ttttgctgat atgtatgaat atattaagga aagaggatat gcaatctatc cgggcaaggt
     6901 gacagaagcg gacacattcc gtatcggaaa tatcggtgag atttatgagg aggatattat
     6961 taaggttaca gatattataa gagactttct ggctgaataa gcagaaaata tagttacaat
     7021 acagggagaa atcatgaaac aggaaaatag atttgatgca gtaatttttg attgggcggg
     7081 aacaacagtg gactacggtt gtttcgcgcc ggtacaggcc tttattgagg tgtttaagca
     7141 ctttggcata gagccgacta tggaagaggt tagaaaaccg atgggaatgt taaagcgaga
     7201 ccatataaag acaatgcttt caatggacag aatctcaaat gagtggcaga aaaagtatgg
     7261 acgcatttgg aaagaggagg atgtggacgc actctatgca ctttttgagg agaagcttat
     7321 gggtatcctg gatggctttg cagatgtaaa gccatatgtg cttgagacag ttgatgagct
     7381 tagaaaacgt ggtataatga ttggctcaac aacaggctac acagataaaa tgatggaaat
     7441 agtagtatca aaagcgaagg aaaacggata tgagcctgat gcctggtttt caccggattc
     7501 aacaggtaac tttggaaggc catatccata tatgattttt aaaaacatgc aaagactcgg
     7561 tgttatgtcg gtagagcgtg tgatgaaggt tggagataca gtttcagata tcagagaagg
     7621 aaggaatgct ggggtgtata cagtaggaat tgttgaagga agttcggagc tggggctgac
     7681 aaaagaagaa tatgataaga tgggggacat agagagaatt gcccgtgtcg aggcggtgaa
     7741 ggaaaggttt cttaaggccg gtgctgatga ggtagtgctt aatatgagaa gtcttatggc
     7801 tataatgtaa agtctgtatt ttgacacccg gtgaaagccg ggtgtttgtt atacgaaaaa
     7861 atagtaaaat tgccttattg ctaacagtaa tctcttagtg tgccggaacg gtaatggtaa
     7921 aagttggaac cagtgcaaat gaagctatag aaattgatag tggaaaaatt gtttacgata
     7981 tcagatttcc gttgtattat aataataaga aacacatctt tataataaat atcgaagaga
     8041 aaaaagtata aaagcatcga agcttggata tggaatatat cttaagctta ttggaggaaa
     8101 gatagtataa tgggaaaaaa agaagcaaag acaaatgcaa taagaatact ggaaacaatg
     8161 aagattccat atgaggcacg cacatacgag tgcgatgact ttgtggatgc agctcagata
     8221 gcagacaagc tgggtcttga ccacgcaggc atgtacaaga ctatcacgac agtcggcaaa
     8281 agcggcggat actatgtgtt tgtggtaccg atagatgacg aaattgattt caaaaaagcc
     8341 gcaaaggcag caggtgagaa gtccatggag atgctgcatc tcaaggattt gacaaaggtt
     8401 acaggctata taaggggcgg ctgtacatca atcggaatga aaaaacagta tcctacattt
     8461 atccatgagg cagctaagga gcaggacaaa atcacagtta gcggaggcag gcttgggctt
     8521 cagattacgc tgtcaccgga tgatttgtgc agggcggcaa aggcagagta tgcagatatt
     8581 ataaaataat taaaatgaga gttctttttc ctaggctttt acacgtcagt gtgttataat
     8641 aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 naataataga actaaagcag aggaataata gagatggaaa aaattatttt aacaggagac
     8761 agaccaacag gaagactcca tataggacat tacgtgggct cacttcgcag aagagttgag
     8821 cttcagaatt caggagagta tgacaaaata tttatcatga ttgccgatgc acaggcactc
     8881 acagacaatt ttgacaaccc tgagaaaatc agacagaata tcatagaggt agcacttgat
     8941 tacctctcag cgggactcga cccggccaag tctacactgt ttgtgcagtc acagataccg
     9001 gagcttacag agcttacatt tttctactca aatctcgtta cagtatcgag acttcagaga
     9061 aaccctacag taaagaatga gattaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttcaagg
     9181 ctacaactgt tccggttgga gaggaccagc tcccaatgat agagcagacc agggagattg
     9241 tcagaaagtt taattctatc tatgatgagg tgctcgttga gccggatgtg cttttacctg
     9301 agaatgaggc atgctgcaga cttccgggta cagacggtgg acagaagatg agcaaatcat
     9361 taggcaactg tatctatctg gcagatacgg aggcagatgt aaagaagaaa atcatgagca
     9421 tgtatacaga tccgacacat atccagattt cagatccggg acatgtggag ggcaatacag
     9481 tattcacata tcttgatgct ttctctaagc cgggacattt tgaggagtat ctgccggagt
     9541 atgctaacct tcaggagctt aaggatcact atacaagagg cggtcttggt gatgtgaaga
     9601 ttaagaaatt cctcaacaac atcatgcagg aggagctttc acctatccgc gcaagaagag
     9661 cagagtatga gaaggatatt ccggctgtat atgagatact aaggaagggc agcgagacag
     9721 caagagaggc tgcagcacag acaatggcag aggttaagcg cgccatgagg ataaattact
     9781 ttgaggatgc agagcttata aagagccaga gtcagaaata cgctgagaag taatattttt
     9841 tatttcaaag acaatacgtt agtgatgtca aaatattttg tggcattatg tgtgtcataa
     9901 aagccacggg gaggcaccat ggtcaagttt tacaggacag atgacaaatt aatacatgag
     9961 ctggatacgc tgcagggagg tacctggata cagatggtca acccttctgt agcagaggga
    10021 cagatggtag cggatgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgcaggat gggtatacgt
    10141 tgattcttgt ggatatacct tcgatagaga ctaggcatga caaggagtca tataccacaa
    10201 tcccgcttgg catcatactt acagatgatg agatagtgac agtctgtaca gaggatacac
    10261 cggtgcttca ggtattttta aataaccgtg taaaggaatt ttccaccaag aaaaagatgc
    10321 gctttgtata ccagattctt tacaggatat ctgtactgta tcagaatgat ctgcgcatca
    10381 tagacaagat gcgtactgag attgaggaga gagtcggaga ggatacagag gaggatgatt
    10441 tgatcgcgct tcacgagctg gaatccacac tcgtatactt tgccacatcg cttcgttcca
    10501 atggagttgt gttagagaga ctgaccagat acaaacgcct tgagcagtat ccggaggata
    10561 aggagcttct cggagacgtg attgtcgaaa ataagcaggc tattgagatg acgggaattt
    10621 acagggacat cataaatggt accagagagc ttatgagcac ggtcattgac aataggctta
    10681 acaatgtcat gaagtcgctc acaatcatca cacttatcat ggctatcccg acagttatct
    10741 caggaatcta tggtatgaat gtcagctcaa agtgggtacc gcttgcaacg acagcgcatg
    10801 gatttatgat tataataggt gttatggttg tcatatgtgt gctgatactg attttattga
    10861 aaaaaaagag aatattgtaa tatattgcgt attcattaaa aggccgcagt atttcataga
    10921 aaataatgaa atactgcggc tgattttatt ttagaactga ttagcagata ctttcaaagc
    10981 gtccgcttgc accacaaggc agcacgagtc cgtttgaaga aaccgggaga ccaatttcgc
    11041 ttgcggttac cttaccatca aactttttaa gtgcagtgct tatcatataa gtgagcactg
    11101 caggctgcaa gccagtcgta taggaattaa taaggaagaa aagcggcttg tcggtaagga
    11161 gctttgcaca aagctgcaca agcggatata cgctttcctc aattttccaa atttcaccct
    11221 taggacctct gccgtatgac ggcggatcca ttatgatggc gtcgtaatga ttgccacggc
    11281 ggatttcacg ctcaacaaac ttcacacagt catccacaag ccatctgata ggtgcgtcac
    11341 caagaccgct gcttgcagcg ttttccttag cccatgtgac catgcccttt gaagcatcca
    11401 catgtgtgac agcggcgcct gcggcagcgg cagcaagagt ggcacctccg gtgtatgcaa
    11461 agagatttaa tacctttaca tcacgaccac ttttttttgc atcataaatc ttctttgaaa
    11521 accagtccca attggcagcc tgctcaggaa acagaccggt gtgcttgaag ctgaatggtt
    11581 ttaagtgaaa tgtgagctct ttattaatag gaagagagta gtttatggtc cactcctgcg
    11641 gcagatcaaa aaactcccat tcaccgccgc ctttcttgct tctgtgatag tgaccgttaa
    11701 gctttttcca gttcttatta gtctttggag tgtcccaaat tacctgagga tcgggacgca
    11761 gcaaaatata tttagaccaa cgctcaagct tttcaccgca tgagcagtca ataacctcgt
    11821 aatctttcca tctatctgct aaccacatat gtttatattc ctttcttatc tgcgtgtaaa
    11881 aaataaacta cacgttgctt gcatttaatc gggtttcttc tataataata tacgtgcaaa
    11941 agtgtaattt ttcaatgaaa tattacccga aataaaaaaa taaatttttt ttgaaaaaag
    12001 tattgcaatt attgtaaacg tcatgtataa tatcatttgc tgtggcatga tagctatgaa
    12061 gtttgaagtt actgtccatg agacagagaa ttcggcggag cgaatgtcaa gttaggaaac
    12121 tgacggcaag tcactgtaca gaacagatat ggtctacaaa atgtagaaac aacgtgtgaa
    12181 agtccacagg acacacacgg agatgtgtac agtcaccgct tgtcgtactg aagttaaaag
    12241 tgcgaaaagg aggcgacttt ttttatggca agtcaagtaa tgagaattac tctcaaagcg
    12301 tatgatcacg agttagtaga ttcaagcgca aagaaaatca tcgagactgt aaagaagaat
    12361 ggatcacagg tgagcggacc ggtacctctt ccaacaaaga aggaagtagt tacaattctt
    12421 agagctactc acaagtacaa ggattctcgt gaacagttcg agcagagaac tcataagaga
    12481 cttatcgata tcatcaatcc aacacagaag acaacagagt ctttatcaag gattgaaatg
    12541 ccagctggtg tttacattga tatcaaaatg aagcagaaat aagcttcaac aattaattag
    12601 tatatccccg aatggtttat atagaagata cagtgcgaaa gcataacgat ctagtaggct
    12661 gctctaggat gaacggttcc gctacttgtt ccggctaatt cttcgcgggc tgggttgaca
    12721 ggcaagaggc gttccgctgt agaaaacagg aggtaaagaa atgaagaaag cgattttagc
    12781 aacaaaaatc ggtatgacac agatcttcaa tgcagatggt gtattagtac ctgtaactgt
    12841 attagaggct ggtccatgtg ttgtaacaca gatcaaaact gttgagaatg acggttacag
    12901 cgcagttcag gttggtttcg gcgacaagaa agagaaagtc gttgagaagg atgccaacgg
    12961 taaaaagagc gtagcacaca gacatggtgt taacaaggct cagatgggac acttcaaaaa
    13021 agcaggtgtt tcaggaaaga gattcgttcg cgagttcaaa ttcgaaaacg cagatgagta
    13081 caatttagca gatgagatca aggctgacat cttcgcagag ggtgacaagg ttgatgtaac
    13141 tgctatttca aaaggtaaag gtttccaggg tgctatcaag agattaggtc agcacagagg
    13201 accaatggct catggttcta aattccatcg tcatcagggt tctaacggtg cttgttcttc
    13261 accaagtaag gtattcaagg gaaaaggaat gcctggacac atgggtagcg taaaggttac
    13321 aactcagaat cttgaggttg taagagttga cgcagacaag aacttactgt taatcaaggg
    13381 cgcagttcca ggagctaaga aagctctcat tacagtaaaa gagactacaa aatctggtaa
    13441 atagtgcaaa gtaagaaagg aggaacttta cgatggctaa cgtagctgta tataacatgg
    13501 aaggcaaaga agtcgacaag atcgagttaa atgattccat tttcggcgta gaaattaatg
    13561 agcatttagt tcatatggct gttcttcagc agcttgcaaa taaacgtcag ggaactcaga
    13621 aagctaagac acgttctgaa gttcgcggtg gcggaagaaa accatggaga cagaagggaa
    13681 caggacatgc acgtcagggt tctacaagat ctccacagtg gacaggtggt ggagttgtat
    13741 ttgctcctac tcctagagat tactcattca agctcaacaa gaaagagaag agagcagctc
    13801 ttaagtctgc acttacatca agagttgttg agaacaagtt cgttgtagta gatgagctta
    13861 agcttgatga gattaagaca aagaaattcg tagaagtatt aaagaacctc aatgttgaga
    13921 aggcactcgt tgtattaaat gatatggacg agaaggttat cgcttctgca gcaaacatcc
    13981 caacagttaa gacaactcag acaaatgagc ttaacgtatt cgatgtatta aaatatgaca
    14041 cagtagtagt tacaaaggct gctgtagcaa caatcgagga ggtatatgca taatggcaaa
    14101 cgtacagtac tatgatgtaa tcctcaaacc agtagtaact gagaagagta tgaatgcaat
    14161 ggctgagaag aaatacacat tccttgttca cccggatgca aacaagacta tgatcaaaga
    14221 agcagtagag agaatgttcg aaggaacaaa ggttgccaaa gttaacacaa tgaactgcga
    14281 aggcaagaac aagcgtcgtg gaatggtaac aggaaagact gctaagacaa agaaggctat
    14341 cgtacagctt acagcagaca gcaaagatat cgagatcttc gcaggactgt agaccgaggt
    14401 cgaaagcaac ttcaaaatga tatgcgtcag aaaagacgcg ataatgaaaa catatcgaaa
    14461 ggagaaacaa aatgggaatt aagacatata acccatatac accttccaga agaaatatga
    14521 ctggttcaga tttctcagag attacaaaga caactcctga gaagtcactt gttacttctt
    14581 taaagaagaa cgctggtcgt aacaatcagg gtaaaatcac agttagacac cagggtggtg
    14641 gaaatagaag aaaatacaga cttatcgatt tcaagagaaa taagaaagac ggtattccag
    14701 caactgtaat tggtatcgag tacgatccaa acagaacagc aaacatcgca cttatctgct
    14761 atgcagatgg tgagaaatca tatattcttg ctccagcagg acttacagac ggaatgaagg
    14821 ttatgagcgg tgctcaggct gaggttagag ttggaaactg cttaccactt gagaatatcc
    14881 cagttggtac acagatccac aacatcgagc ttcttcctgg aaagggcgga cagcttgttc
    14941 gttcagcagg acttagcgca cagcttatgg ctaaagaagg caaatatgca acacttcgtc
    15001 ttccttcagg agagatgaga atggttccaa ttcagtgccg tgctacaatc ggtgtaattg
    15061 gaaacggaga tcacaacctc gtaaatattg gtaaagccgg acgtaagcgt cacatgggtg
    15121 ttcgtccaac agttcgtgga tctgttatga accctaacga ccatccacac ggtggtggag
    15181 aaggtaaggc acctgtagga cgtccaggac catgtactcc atggggcaaa cctgcacttg
    15241 gcttaaagac cagaaagaag aacaaacagt ctaacaagat gatcgttcgt agaagagacg
    15301 gtcgtgcaat caaataatta ggaggttgaa taaatggcac gttcattaaa aaaaggacca
    15361 tttgctgatg aaagcttact taaaaaagta gatgctatga acgcttctgg tgacaagtct
    15421 gtaatcaaga catggtcacg tcgttcaaca atcttccctt cattcgttgg acacacaatc
    15481 gctgttcatg atggaagaaa gcatgttcct gtatatgtta cagaggatat ggttggacat
    15541 aaacttggtg agttcgttgc aacaagaact tacagaggac atggaaaaga cgagaagaaa
    15601 tcaggcgtaa gataagattt attgaaagga ggtttcatcc atggcaaagg gacatagatc
    15661 caaaatcaag agagagagaa atgaagttag agatacaaga ccttcagcaa agttatctta
    15721 tgctagagtg tctgtacaga aagcttgttt cgttttagat gctattcgtg gaaagagtgt
    15781 taacgaagca cttgcaatcg taacatacaa tcctagatat gcttcaagct tagtaaagaa
    15841 attattagag tcagcaatcg ctaacgcaga aaataattat ccggaaaaga actataaggc
    15901 agagaacctt tacgttgcag agtgttttgc aaataaagga ccaacaatga agagaattca
    15961 tcctagagca cagggtagag cttacagaat cgagaagaga atgagccaca ttacaatcgt
    16021 gcttgatgaa agataggagg caatcatggg acagaaggtt aatcctcatg gcttaagagt
    16081 tggcgttatc aaggactggg attccaaatg gtatgcagag ggtgatttcg ctgattactt
    16141 agtagaagat tataatatca gaacattttt aaagaaaaaa ttatacgcag ctggtgtttc
    16201 aaagattgag atcgagagag cttcagaccg cgttaaggtt atcatttata cagctaagcc
    16261 gggtgttgta atcggtaagg gcggagctga gatcgagaag atcaaggctg aggttcagaa
    16321 gcttacagac aagaaattag ttgttgatat caaagaagta aagagaccgg acaaggatgc
    16381 acagcttgtt gctgagaaca tcgctttaca gcttgagaac cgtgtttcat tccgtagagc
    16441 aatgaagtct tgcatgggaa gagctatgaa ggccggcgtt aaaggaatta agacaacatg
    16501 ttcaggtcgt cttggcggag cagatatggc tcgtacagag acatacaatg atggaactac
    16561 tccactccag acaatcagag cagatatcga ttatggattc gctgaggcag atacaactta
    16621 cggcaaggtt ggcgttaagg tttggatcta caaaggcgag gtacttccta caaaagcaaa
    16681 caaggaagga ggagctaagt aattatgtta atgcctaaga gagtaaaaca tcgtaaacag
    16741 ttccgtggtt ccatggcagg aaaagctaca agaggaaaca agatcacaaa tggtgagtac
    16801 ggtattgtag cacttgagcc atgctggatc aaagcaaatc agattgaggc tgcccgtgta
    16861 gctatgactc gttacatcaa gcgtggtggt aaagtttgga taaagatttt cccagagaaa
    16921 cctgtaacac ataagcctat gggtgttcga atgggtaaag gaaagggtgc ccttgagtat
    16981 tgggtagctg tagtaaaacc aggtagagta ttatttgaaa tctccggagt accggaagac
    17041 gtagctaaag aagctttacg tcttgctaca cacaaattac cttgcaagtg taaagtagtt
    17101 tctcgtgcag atttagaagg cggtgagtca aatgaaaact agtgcatacg ttaaagagtt
    17161 acaggcacag agcgtagagc agttacaggc acagctcgtt gatgctaaaa aagaactttt
    17221 caatttaaga ttccagaatg caactaacca gttagataac acagcaagaa tcaaagaggt
    17281 tcgtaagaac atcgctagaa ttcagactgt tatcactgca aaggctgcag aatagtttat
    17341 cctatcagaa aggagatttt agaccgtgga agaaagaaat cttagaaaaa cacgtgttgg
    17401 tgttgttgta agcgataaga tggataagac aatcgttgta gctgtaagag ataacgtaag
    17461 acatcctctt tacaacaaga tcgttaagaa gacttataaa ttaaaagctc atgacgagaa
    17521 aaacgatgca aagatcggtg atacagtcag agttatggaa acaagacctt tatctaaaga
    17581 taagagatgg agacttgtag aaatcatgga aagagcaaaa taataaaagg aggctacaag
    17641 catggtacag caggaaagca gactcaaggt tgctgataac actggcgcta aagagttact
    17701 tgttatccgc gttatgggtg gatctaccag aagatatgcg aacatcggtg atgtaatcgt
    17761 tgctactgtt aaagatgcaa caccaggcgg tgttgttaaa aaaggcgatg tagttaaagc
    17821 cgtagtagtt cgttcagtaa aaggtgctcg tcgtaaagac ggatcttata taaagtttga
    17881 tgagaacgca gcagtaatca tcaaagatga caaaactcca aaaggaacac gtatatttgg
    17941 accagtagct cgtgagcttc gtgagaaaca gttcatgaag attgtttctc tggctccgga
    18001 agtattatag gaggaacgtc aatggcaact atgaaaatca aaaaaggcga ccttgttaag
    18061 gttatcgctg gaaaagatat caacaatgag ggcaaggtaa ttgccgtaaa cgcaaagaac
    18121 aatacacttc ttgttgaggg aatcaacatg gttactaagc atacaaaacc aagtatgtca
    18181 aatcagcagg gcggtatcgt tcatcaggag gctccaattg atgcttccaa cgtaatgctc
    18241 atccatgacg gaaagcctac tcgtgtagga ttcaagatgg atggagataa gaaagttcgc
    18301 tttgctaaat caacaggcaa ggttatcgac taattggaga gaggaggatt aagacattga
    18361 gtagacttag agaacagtac gaaaatgaga tcaaggacgc aatgatcaaa aagttcggat
    18421 acaaaaacgc aatggaaatt ccaaagcttg acaagatcgt agttaacatg ggtgttggtg
    18481 aagctaagga aaacgctaag gttcttgagg cagccgttaa ggatatggaa gccatcacag
    18541 gacagaaagc agttacaact aaagcaaaga atgctatagc taactttaaa atcagagaag
    18601 gaatgccaat cggatgtaag gttacattac gtggtgagaa gatgtacgag tttgcagatc
    18661 gtctcatcaa cttagcatta cctcgtgtac gtgacttcag aggtgttaac cctaacgcat
    18721 ttgatggccg tggaaactat gcccttggaa ttaaagagca gatcatcttc cctgagatcg
    18781 agtatgataa ggtagataag gttcgtggta tggacattat cttcgttact acagctaaga
    18841 cagacgaaga ggcacgtgag ttactggcac agttcaatat gccattcgct aaataatcgg
    18901 aggaattcta atggctaaga caagtatgaa aatcaaacag cagcgtgcac caaagttctc
    18961 tacaagagct tatacacgtt gtagaatttg tggacgtcca cattcagttt taaagaaata
    19021 cggaatctgc agagtatgct tccgtgagtt agcatacaag ggacagatcc caggcgttaa
    19081 aaaagctagc tggta
//
LOCUS       DPUF01000019            3998 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_15531, whole
            genome shotgun sequence.
ACCESSION   DPUF01000019 DPUF01000000
VERSION     DPUF01000019.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_15531"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            41..1066
                     /locus_tag="DHV81_00630"
     CDS             41..1066
                     /locus_tag="DHV81_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCI93056.1"
                     /translation="MSRKSKILSNAELSAFCQQISLIIAAGLPTYYGVSILCDEAPDK
                     QTRDLLEQIYKPMELGATLHSALKDTGYFPKYMINMIQLGEETGRLEEVLNSLSKYYD
                     REDEIASGIKSAVAYPLVLSAVMIAVIVVMIAKVLPVFSQIYAELGSELTGTAAVLMK
                     LSEYINRYLLVIIAVMLFAALVLFIFFKTRFGKKLLMKTALSMSIASSRFANCMFLAL
                     SSGLDTDRGLDLAQALVNNPVLDSKINDCRKNISNGETFSEALLHSNIFSKMYSSWIA
                     IGSKTGSMDEVMQHICTSCEDDTDARLSRFISVIEPAMVILLCIFIGFILVSFLLPLL
                     GIISSIG"
     gene            1083..1403
                     /locus_tag="DHV81_00635"
     CDS             1083..1403
                     /locus_tag="DHV81_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93057.1"
                     /translation="MLFFVQKKKSTIWKFIPIVILAACTVLFGAFSSKLSDDNTKRSK
                     STLERALTRSITQCYALEGTYPPDINYLTDHYGLTYNSDYYYIDYQYIGSNLRPDVTI
                     IERK"
     gene            1407..1889
                     /locus_tag="DHV81_00640"
     CDS             1407..1889
                     /locus_tag="DHV81_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HCI93058.1"
                     /translation="MFKLKSRSHQADSFFVMLLFFLFALTAFILVLIGVKQYKSTANA
                     MNYNYEVRTVTSYLREKVRQNDSDAAISVETINGTKALCLKNTINDIVYNTFIYYYGG
                     SLRELYVQDGSSYSLDSGQRIVEIGGLDMTETTDKMITVTITDTSGGTTDTYCSVNSD
                     "
     gene            1985..2452
                     /locus_tag="DHV81_00645"
     CDS             1985..2452
                     /locus_tag="DHV81_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93059.1"
                     /translation="MISTKLKQSKNRIFLLEITINLLLFSALLIVGLLFFIKTHKLTE
                     ETQVLHEAVNACSNAASAYEQEHGDLNAVSSLFDGSICADEKLFIYLDKSYHPCDKDD
                     AAYKVIVSRLGQDAYGVQKADIEFIAADSHCVYSITACSYTALSSDGRTVSTP"
     gene            2449..>2688
                     /locus_tag="DHV81_00650"
     CDS             2449..>2688
                     /locus_tag="DHV81_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93060.1"
                     /translation="MKKRREFSNFGFSTILLTFSMICIVTFSALAFVTANSDYKLSKR
                     VADNNSSYYRACEKVWDEISQIDAILASAYDGSPDK"
     assembly_gap    2689..2795
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2796..2996
                     /locus_tag="DHV81_00655"
     CDS             <2796..2996
                     /locus_tag="DHV81_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93061.1"
                     /translation="NESNGKVTGLTYTITCTVSDTQTLTVKLDINYPVHRQDAFYKIK
                     QWKLNTDTAFEENDTLNLIGGN"
     gene            2998..>3998
                     /locus_tag="DHV81_00660"
     CDS             2998..>3998
                     /locus_tag="DHV81_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HCI93062.1"
                     /translation="MDVKKILKEATDNGASDIFIVAGRPLTYKSKGVMECLNEIRMIP
                     KETFEFVTSIYALAERDISKFEETGDDDFSFAIPGVSRYRVSTFKQRGSYSAVIRVIS
                     FTLPKPSDLHIPDSVMELAETNNGMVLVTGPAGSGKSTTLACIIDQINHEKEEHIITL
                     EDPLEFLHRHDKCIVSQREINTDTESYLTALRAALRQSPDVILLGEMRDYETINTAVT
                     AAETGHLIFSSLHTIGAANTIDRIIDAFPASQQHQIAIQLASVLQAVVCQKLLPATNG
                     EMVPAFEIMVLTPAIRNLIREGKVHQIDGIIYTSAAENMIAMDTSIFNLYKAGVISK"
BASE COUNT         1163 a          886 c          796 g         1046 t
ORIGIN      
        1 agtgattaca ttaccaaata cgtctattag gaggacataa atgtctagaa aatccaagat
       61 tttaagcaat gcagaacttt ctgccttctg tcagcagatt tctcttatca tagcagccgg
      121 gcttcctaca tactatggtg tttccatatt atgtgatgaa gcacctgata agcagacacg
      181 tgatctactt gagcagatat ataagccaat ggagcttggt gcaacgcttc acagtgcact
      241 taaagataca ggatactttc caaaatatat gataaatatg atacagctgg gtgaagaaac
      301 cggccgtctt gaagaggtct taaactcact ctcaaagtac tatgaccgcg aagacgaaat
      361 agcttcaggc atcaaaagtg ccgttgccta tcctctcgtg ctttctgccg tgatgattgc
      421 cgttattgtc gtaatgattg ccaaggtttt acctgtattt tcacagatat atgcagagct
      481 tggaagtgag cttaccggca ctgctgccgt gctcatgaag ctcagtgaat acatcaatag
      541 atatttgctt gtcattatag ctgtcatgct ctttgccgca ctggttcttt ttatattttt
      601 taagacacgc ttcggaaaaa agctccttat gaaaactgct ctttccatgt caatcgcatc
      661 cagccgcttt gcaaactgca tgtttttagc actctcaagc gggctcgaca cagaccgtgg
      721 tcttgatctg gcacaggctc ttgtaaacaa tcctgtcctt gattctaaaa taaatgactg
      781 ccgtaaaaat atatctaacg gcgagacatt ttcggaggca ctgctgcact ccaatatttt
      841 ttcaaagatg tactcaagct ggattgcaat cggcagcaaa accgggtcta tggacgaggt
      901 catgcagcat atatgtacat cctgtgagga tgacacagat gcaaggctgt cacgattcat
      961 atcagtgatt gaacctgcca tggttatcct gctttgtata ttcattggct tcatactagt
     1021 atcattttta ctcccactgc ttggaattat atcaagcata ggatagctta cggaggcaca
     1081 gtatgctgtt ttttgttcag aaaaagaaat caaccatatg gaaattcata ccgattgtga
     1141 ttttggccgc atgtaccgtt ttgtttggtg ctttctcttc aaagctgtca gatgacaata
     1201 ccaaaagaag caagagcacg ctggaacgcg ccctcacacg aagtatcaca cagtgctatg
     1261 ctcttgaggg aacataccct ccggacataa attatctaac agaccactat ggtctcacat
     1321 acaacagcga ctattattac atagactatc aatacatcgg ctccaatctg cgtcccgatg
     1381 tgacaataat agagaggaaa taacccatgt tcaagctaaa atcaagatca catcaggctg
     1441 acagtttttt tgtgatgctg ctgttttttc tttttgcgct gactgctttt atcctcgtgc
     1501 tgattggcgt aaagcaatac aagtccactg ccaatgccat gaattacaac tacgaggtac
     1561 gcactgtcac atcttatctg cgcgaaaaag tcagacagaa tgattctgac gctgcaatca
     1621 gtgtagaaac aataaatggc acaaaggctc tatgtctgaa aaacacaata aatgacatcg
     1681 tgtataatac tttcatatac tattatggcg gttctctaag agagctctat gtacaggatg
     1741 gttcatcata cagtctggac agcggacaaa ggattgtcga aatcggggga cttgacatga
     1801 cagaaaccac cgacaaaatg attaccgtaa ctatcaccga tacatccggt ggcactaccg
     1861 atacctactg ctccgtcaat tccgactaac aatcgtccga caataaatgc agcaatatca
     1921 cgtacaccgc tccctgatat gccactcgat gccacacaaa cttacataca acaaggaata
     1981 aaccatgatt tcaacaaagc ttaaacaatc gaaaaacaga atattcctgc ttgagattac
     2041 cattaatctg ctgctttttt cagctctttt gattgtggga ctgcttttct tcataaagac
     2101 ccacaagctg acagaagaaa ctcaggtgct gcatgaggct gttaatgcat gcagcaatgc
     2161 agcaagcgca tacgaacagg agcatggcga tttaaacgcc gtatcatcgc tttttgacgg
     2221 ctccatctgt gcagatgaaa agctattcat ttatcttgac aaaagctatc atccatgtga
     2281 taaggatgat gccgcataca aggttattgt aagcaggctt gggcaggatg cctacggagt
     2341 acaaaaggct gacatagagt ttatagcagc agacagccac tgcgtataca gtattaccgc
     2401 gtgcagctat actgcacttt cctctgatgg aaggacggtg agcaccccat gaaaaaaaga
     2461 agagagtttt caaatttcgg tttttcgacc atcctgctta ccttttccat gatatgcatt
     2521 gtcacgttct cggcactcgc ctttgtcaca gcaaactctg actataagct gagcaaacgc
     2581 gttgctgata acaacagctc gtattaccgt gcatgtgaaa aggtatggga tgagatatca
     2641 cagattgatg ccattcttgc atccgcctac gatggcagtc ctgataaann nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaatga atcaaacggc aaggttaccg
     2821 gtctgaccta taccatcacc tgtactgtaa gtgatacgca gactctcact gtaaagcttg
     2881 atatcaacta tcctgtccac aggcaggatg ctttttataa aataaaacaa tggaagctca
     2941 ataccgacac agctttcgag gaaaatgata cactaaactt aattggagga aactaaaatg
     3001 gatgttaaaa agatattaaa ggaagcgact gacaacggag catcggatat tttcatagtg
     3061 gccggccgcc ctctcacata caaatcaaag ggagtgatgg agtgcctcaa tgaaatcaga
     3121 atgattccaa aagaaacctt tgaatttgtc acatcaatct atgctcttgc cgagagagat
     3181 atttcaaaat ttgaggaaac cggagatgat gacttttcct tcgctatacc gggagtatca
     3241 cgttacagag tgagcacttt caagcagcgt ggctcttact ctgctgtcat ccgtgttatc
     3301 tcattcacac tgccaaagcc aagcgattta catataccgg actctgttat ggagcttgcg
     3361 gaaacaaaca acggcatggt gctggttacc ggcccagccg gaagcggtaa atctaccaca
     3421 ctagcatgta ttattgatca gatcaaccat gagaaggaag agcatattat cactcttgag
     3481 gatccgcttg agtttttaca tcggcatgac aaatgtattg tgtcacagcg tgaaatcaac
     3541 acagatacag aaagctatct cactgcactt cgcgccgcac tccgccagag tcctgacgtc
     3601 atattgcttg gagaaatgcg agactatgag accatcaata cagctgtcac agcagctgaa
     3661 accggtcatc tcatattttc atcacttcac acaattggtg ccgcaaatac cattgaccga
     3721 ataatcgatg cgtttcctgc ttcccagcag caccagatag ccatccagct tgcatctgtg
     3781 ctgcaggctg tcgtctgcca gaagctttta cctgccacaa acggtgagat ggtgcctgct
     3841 tttgaaatca tggtactcac gcctgcaata aggaacttaa tccgtgaagg caaggtccat
     3901 cagattgacg gtatcatcta cacctctgca gctgagaata tgattgcaat ggatacaagc
     3961 atcttcaatc tgtacaaggc cggtgtaatc agcaagca
//
LOCUS       DPUF01000020            3007 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23247, whole
            genome shotgun sequence.
ACCESSION   DPUF01000020 DPUF01000000
VERSION     DPUF01000020.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23247"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="DHV81_00665"
     CDS             <1..435
                     /locus_tag="DHV81_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCI93063.1"
                     /translation="HQTVKKVTNDFEALGYNTAISQMMIFVNEVYKAGRCPREFAEGL
                     IKMLSCVCPHVGEEIWQRMGHDDTIAFEPWPVYDEAKTVEDTVEIAVQINGKTKGTLA
                     IGKQDPKDEVIAKAKDVIADKLTGNIVKEIYVPGRIVNIVMK"
     gene            complement(491..1153)
                     /locus_tag="DHV81_00670"
     CDS             complement(491..1153)
                     /locus_tag="DHV81_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93064.1"
                     /translation="MDFVLGHGASYMEADIRDMTDKEGFCRAHFKKMFDYGNSLGNAW
                     ILKTMYMRHIEEMDKEFKNFKPDSVQRSGLFKKANASSNSIVDWINKRESTCFICDSV
                     NKTFNAYMKTFFTMYEKDPELKEKLKNTKGFCLDHLKVVLEGADTYLKGDKRKEFYDI
                     VLPLMHDNMNRIYEDVAWFIEKYDYKNKDADWKNSKDAIQRGMQKLRGSDPSLPPHVL
                     KK"
     gene            complement(1283..2188)
                     /locus_tag="DHV81_00675"
     CDS             complement(1283..2188)
                     /locus_tag="DHV81_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI93065.1"
                     /translation="MNQNLSSYWIFYTVANAGNISKAAKELYISQPAISKSIQKLEDG
                     LECKLFSRSSRGVVLTDEGRLLYDHVSEAFEALNEGEEKLKRSIELGVGQLKIGVSST
                     LCKYMLLPYLKEFIHRNPHISISIECQSTNETLKLLDDDKIDIGLIGKPASLKTFNFY
                     YLDNIEDIFVTTKDYLRNLSARGVKKSEILQNSTLMLLDKGNMTRKYIDDYLQENQIH
                     VKDSIDISNMDLLIDFAKIGVGVACVIKSIVSKELADGTLIEIPLGIPIHKREVGFAY
                     KESSKLSTSLQTFIKFYESYTPEDF"
     gene            2415..>3007
                     /locus_tag="DHV81_00680"
     CDS             2415..>3007
                     /locus_tag="DHV81_00680"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596727.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCI93066.1"
                     /translation="MGMTMTQKILAAHAGLDSVSAGQLIEADLDLVLGNDITSPVAIH
                     EINKMKVEGVFNKDKIALVMDHFAPNKDIKSAEHCKCVREFACKNEITNYFDVGKMGI
                     EHALLPEQGLTVAGDVIIGADSHTCTYGALGAFSTGVGSTDMAAGMATGKAWFKVPSA
                     IKFNIVGKPAEWISGKDVILHIIGMIGVDGALYKSME"
BASE COUNT          841 a          564 c          687 g          915 t
ORIGIN      
        1 catcagacag taaagaaggt cacaaacgac ttcgaggcac ttggctacaa tacagctatc
       61 agccagatga tgatttttgt caatgaagta tacaaggcag gcagatgtcc tagagagttt
      121 gcagagggac ttatcaagat gctttcttgt gtatgtccac atgttggtga ggaaatctgg
      181 cagagaatgg gacatgacga tacaatcgca tttgagccat ggccggtata cgatgaagca
      241 aagactgttg aggatacagt agagattgct gtacagatca acggaaagac taagggcact
      301 cttgcaatcg gaaaacagga tcctaaggat gaggttatcg caaaagcaaa ggatgttatt
      361 gcagataagc ttacaggaaa tatcgtaaaa gagatttatg ttccgggacg tattgtaaac
      421 atcgtaatga aataaaaatt acccggtatt ttgcacagcc attagatggc cggcagaata
      481 ccgggtgatt ttattttttg agaacatgtg gtggaagaga tggatcactt ccgcgaagct
      541 tctgcatacc gcgctggatc gcatccttgg aatttttcca gtcggcatct ttatttttat
      601 aatcatattt ttcgatgaac catgcaacat cttcatatat gcggttcatg ttgtcgtgca
      661 ttagagggag cacgatatcg taaaattctt tgcgcttatc accttttaaa taagtgtcag
      721 ctccttccag aaccactttc aggtgatcga gacaaaatcc ttttgtgttc tttaattttt
      781 cttttagttc aggatccttt tcatacatgg tgaagaaggt cttcatgtat gcattaaagg
      841 tcttgtttac gctgtcacat ataaagcagg ttgattcacg tttgttaatc cagtccacga
      901 tggaattgga ggaggcgttt gcttttttga aaagaccgct cctttgcact gaatcaggct
      961 taaagttctt aaattctttg tccatctcct caatatggcg catgtacatg gtttttagta
     1021 tccaggcgtt tccgagtgaa ttgccatagt caaacatttt tttgaaatgt gcacggcaga
     1081 agccctcctt atcggtcata tcgcgtatgt cagcctccat gtaagaggca ccatgaccaa
     1141 gtacaaaatc catgacgtga tcctcggtgc gtttttctat atagcagaag gggcattcgc
     1201 cggcattgtc aaaggcgtct gatatgggga tagtgtgtat ttgttctttc aaggtaatac
     1261 ctccggggtc atattttgtt atttaaaaat cctctggtgt gtagctctca taaaacttga
     1321 taaaggtctg cagagaggtg gaaagctttg aagactcctt gtaagcaaag ccaacctcac
     1381 gcttgtggat tggtatgccg agaggaattt caatcagggt accgtcagca agctcctttg
     1441 aaactatgct ttttatgacg caggccacac ctacgcctat tttggcaaag tcgatgagca
     1501 ggtccatatt tgatatatct atagagtcct ttacgtgtat ctggttctcc tgcagataat
     1561 catcaatata tttacgtgtc atattgcctt tatcgagaag catgagagta gagttttgca
     1621 aaatctcgct ctttttgaca ccacgggcgc ttaagttgcg cagatagtcc tttgtagtga
     1681 caaatatatc ctcgatgttg tccagataat agaaattgaa agtcttaagg gatgctggtt
     1741 ttccaatcag acctatatct attttgtcat catcgaggag ctttaaggtc tcattggtgg
     1801 actgacattc gattgagatg cttatatgag gatttctgtg tatgaattcc ttgagatatg
     1861 gcaaaagcat atatttgcag agggttgagc tgacaccaat tttaagctgg ccgacaccga
     1921 gctctattga gcgcttcagc ttttcctcac cctcatttaa tgcctcaaag gcctcgctta
     1981 catgatcata cagaagcctg ccctcatcgg tcaagacgac acctctggag cttctggaaa
     2041 aaagcttgca ttccagtccg tcctcgagct tttgaattga cttgctgatg gcaggctggc
     2101 tgatatagag ctccttggct gcctttgata tattgccggc attggcgaca gtatagaaaa
     2161 tccagtaaga tgagagattc tggttcattt gtacctcctg tggtttgtac ttttaatggg
     2221 ctgtaatcgt tgcttgtcac aaaaatgtgt gaacatcaac aaatctgtgt ttattatacc
     2281 atgagattta gttatggtaa atatgtttat tatgtatttt tattatggta aatcatatgt
     2341 tatgatgtct taagtaaact tgattcagat acaatcataa tacaatattt ttatatgcta
     2401 ggaggaatat aagcatggga atgacaatga cacagaagat tcttgctgca cacgcaggcc
     2461 ttgactctgt tagtgcagga cagctcattg aggctgattt agatcttgtg cttggaaacg
     2521 acattacctc acctgtagcg attcatgaga tcaacaagat gaaggtagag ggagtgttta
     2581 ataaagataa aattgcactc gttatggatc actttgcacc aaacaaggac atcaagtcag
     2641 cagaacactg taaatgtgtt cgcgagtttg cctgcaaaaa cgagattaca aattactttg
     2701 atgtaggaaa aatgggcatc gagcatgcac ttttacctga gcagggactg actgtagcag
     2761 gtgatgtgat tataggagct gattctcata catgtacata cggagcttta ggagcattct
     2821 ctacaggtgt gggaagcact gatatggcag caggaatggc tacaggcaag gcctggttta
     2881 aggtaccttc tgctatcaag ttcaatatcg tcggaaaacc ggctgagtgg attagcggca
     2941 aggacgtcat ccttcatatc atcggtatga ttggtgtgga cggtgcactt tataagtcaa
     3001 tggagtt
//
LOCUS       DPUF01000021            3285 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_18479, whole
            genome shotgun sequence.
ACCESSION   DPUF01000021 DPUF01000000
VERSION     DPUF01000021.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_18479"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="DHV81_00685"
     CDS             complement(<1..306)
                     /locus_tag="DHV81_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCI93067.1"
                     /translation="MDNSNNNGLEYDDNSDMSDKKPHRKGFRNGFGVGIATGIVTTIV
                     LGLIVLFAYTMITGRSVHTVAKSADVLDDKTVQKIDELANYIDEYYYEDYDKDDLENG
                     "
     assembly_gap    456..665
                     /estimated_length=210
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<666..1300)
                     /locus_tag="DHV81_00690"
     CDS             complement(<666..1300)
                     /locus_tag="DHV81_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI93068.1"
                     /translation="MRINTFFYSIKQGFKNIFRNKMFSLASIATMAACIFMFGLFYIV
                     VTNFSSMVKTAEEGVAVTVFFNEGTTEDVIKADKAQIEKRAEVGKVDYQSAADAWSDY
                     QKEYFEGYDDAAASFGDDNPLSNSANLQVYLNDVSMQQTLVNYIKGLDGVRKVNQSQD
                     VANTLTDLNKLISYVSGGIILILLCVAIFLISNTVTTGIAVRREEIAIMKL"
     gene            complement(1290..1973)
                     /gene="ftsE"
                     /locus_tag="DHV81_00695"
     CDS             complement(1290..1973)
                     /gene="ftsE"
                     /locus_tag="DHV81_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCI93069.1"
                     /translation="MIKLEHVSKAYSAGIPALNDVNLEIDDGEFVFVCGDSGSGKSTL
                     IKLLLKELDPTDGTITVDGRNLTRIRHKQVPKYRRNVGVVFQDFRLLKDRNVYENVAF
                     ALKVIGASTAVIKKKVPQMLSMVGLAAKYKSKPRELSGGEQQRVAIARALVNEPSMLL
                     ADEPTGNLDNNNAWEIMKLLEEINEKGTTVIVVTHNLEIVKVMKKRVITIKKGLVVSD
                     SKDGDSDED"
     gene            complement(1984..3069)
                     /locus_tag="DHV81_00700"
     CDS             complement(1984..3069)
                     /locus_tag="DHV81_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HCI93070.1"
                     /translation="MISNQILQNTIDGLKGITRTDLCVIDVEGKILAATFPNAEAFIE
                     PAQAFVASPADSQVINGCQFFKVFDDHQLEYVLLAYGDSEDVYMIGKIASFQIQNLLV
                     AYKERFDKDNFIKNLLLDNLLLVDIYNRAKKLHIDIEVRRVVFIVETNREKDGNELEK
                     IRSLFGGKSKDFVTAVDEKNIIVVKELAENETYDDLRKTAEVILNLFRSEADGVHIAY
                     GTVINELKEVSRSYKEARMALDVGKIFFEERNIIAYSQLGIGRLIYQLPIPLCKMFIK
                     EIFDGKSPDDFDEEILSTINKFFENSLNVSETSRQLYIHRNTLVYRLDKLQKSTGLDL
                     RVFEDAITFKIALMVVKYMKYMESMDY"
BASE COUNT          866 a          748 c          491 g          970 t
ORIGIN      
        1 accgttttcc aaatcatcct tatcgtaatc ctcatagtag tactcgtcta tgtagttcgc
       61 aagctcatcg attttctgca cggtcttgtc gtcaagcaca tctgcgcttt ttgcaacggt
      121 atgcacgctc ctgcctgtaa tcattgtgta tgcaaagagc acaatcaatc caagcacaat
      181 ggtggtcact atacctgtag caatcccgac accgaaccca tttctaaatc cctttctatg
      241 aggcttttta tcgctcatat ctgaattatc atcgtattct aagccgttgt tgtttgaatt
      301 atccatttca gtcctgtcct ctctatatta tgatatgaac ttaaaacagc ctgtctccga
      361 tcggagccag gccgtcataa ttattattat actcttaaat gtttccgaat tgtgaatcgg
      421 cttcccaaaa agccaattcc aacgcccagt ataagnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnataag cttcatgata gctatttcct ctcgtctgac cgcaataccc gtagtaactg
      721 tgttgcttat aaggaaaatc gcaacacaca gaagaatcag aatgatacct cccgatacat
      781 agctgataag cttatttaaa tcggtcagtg tattggccac atcctgcgac tggtttacct
      841 ttctgacgcc atccagcccc ttgatatagt tgacaagagt ctgctgcata gagacatcat
      901 tcaaataaac ctgcagattt gctgaattac tgagagggtt atcatcacca aatgaagctg
      961 cagcatcatc gtagccctca aaatactcct tctggtaatc actccatgca tcggccgccg
     1021 actgataatc caccttgcca acctcagccc tcttttctat ctgagccttg tccgctttta
     1081 ttacatcctc tgtagtgccc tcattaaaga acactgtgac agcaacaccc tcctcagctg
     1141 tcttaaccat gcttgagaag ttcgtcacaa ctatataaaa cagaccaaac ataaaaatac
     1201 aggctgccat tgtcgctatg gatgccagtg aaaacatctt gtttctgaat atattcttaa
     1261 atccctgctt gattgaataa aaaaacgtat taatcctcat cactatcacc atccttgctg
     1321 tcagatacaa caagaccttt ctttatagtg ataacacgtt ttttcataac cttaacaatc
     1381 tcaagattgt gagttacaac gataacagtt gtgcctttct cgttgatttc ctcaagcagt
     1441 ttcataatct cccacgcatt gttattgtcc agattacccg tcggctcgtc agcaagcagc
     1501 atgctcggct cattgacaag agctcttgca atagctactc tctgctgctc tcctcccgaa
     1561 agctctctcg gctttgactt gtactttgca gcaagcccta ccatagaaag catctgtggc
     1621 acctttttct taataacagc tgtggatgca cctataacct tgagtgcaaa agctacattt
     1681 tcataaacat ttctatcctt taataatctg aagtcctgga aaacgactcc aacatttctt
     1741 ctgtatttag gaacctgctt gtggcgaatg cgcgtcagat tccttccgtc aacagtaatc
     1801 gttccgtctg taggatcaag ctcctttaaa agaagcttaa tcaaagtgga tttgccggat
     1861 ccgctatctc cgcatacgaa aacaaactct ccatcatcaa tctcgagatt gacatcattg
     1921 agagcaggta tacctgcaga atatgcctta ctgacatgtt caagtttaat cattactttc
     1981 tccttaatag tccattgatt ccatatattt catatatttg actaccataa gggcaatctt
     2041 aaatgtgatg gcatcctcga acactctaag gtcgagtcct gtacttttct gaagcttgtc
     2101 caatctatat acaagagtat ttctgtgaat gtagagctgt cttgaagtct cagatacatt
     2161 caggctattc tcaaagaact tatttatagt agataatatt tcttcatcaa aatcgtcagg
     2221 ggatttgcca tcgaatatct ctttgataaa catcttgcat agaggaatcg ggagctgata
     2281 tatcagacga cctattccaa gctgggaata tgctataata ttcctctcct cgaaaaagat
     2341 ttttccgaca tcgagagcca tcctggcctc cttatatgag cgcgacacct ctttcaactc
     2401 attgataaca gtaccatatg caatatgtac cccatccgct tcggatctga acagattcaa
     2461 aatcacctcg gctgtctttc tgagatcatc ataggtctcg ttttctgcca gttccttcac
     2521 tactattata ttcttctcgt caacagctgt aacaaaatcc tttgatttgc cgccgaaaag
     2581 acttctgatc ttctcaagct cgttgccgtc cttctccctg tttgtctcaa caataaatac
     2641 tacacgtcgc acttcaatat caatatgaag cttcttcgcg cgattgtata tatcaaccag
     2701 aaggagatta tccaaaagca gattctttat aaaattatcc ttgtcaaatc gctccttgta
     2761 cgcaaccaga agattctgaa tctggaagct tgcaatcttg cctatcatgt agacatcctc
     2821 gctgtcacca taagctaaaa gcacatattc cagctggtga tcatcaaaga ctttgaaaaa
     2881 ctggcatcca ttaatcacct gactgtctgc tggtgatgca acaaaggcct gtgccggctc
     2941 tataaacgcc tctgcgttcg gaaatgtagc tgcaagaatc ttgccctcaa catctatcac
     3001 acaaagatcg gttctcgtaa ttcctttaag gccatcaatt gtattttgaa gtatctgatt
     3061 ggaaatcata aatcgttctc cttctcccct ttttgtaact gtgcattgcc ctcacagtca
     3121 ctttataact aattgcataa atacttgcta ttattttaat acattcgcac aaaaaaataa
     3181 agaggtaaat gtacaatttt tacacatttc ctcttatttt ttcttctatt gttataagaa
     3241 ttaactatat ctaactgttt tatctatgtt cataggtgtt gataa
//
LOCUS       DPUF01000022            3096 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_65415, whole
            genome shotgun sequence.
ACCESSION   DPUF01000022 DPUF01000000
VERSION     DPUF01000022.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_65415"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="DHV81_00705"
     CDS             <1..489
                     /locus_tag="DHV81_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HCI93071.1"
                     /translation="GDHMSMYAVNASVPKTLVRHLVKYYADTCGSDLLERTLLDVLDT
                     PVSPELLPPENGKISQKTEDLVGPYELHDFFLYNMLRCGYAPAKVYRLARIAFEGKYD
                     DEFILKWLKNFYRRFFAQQFKRSCLPDGPKVGTVAVSPRGDLRMPSDACGRIWMDEVD
                     KL"
     gene            506..1465
                     /locus_tag="DHV81_00710"
     CDS             506..1465
                     /locus_tag="DHV81_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HCI93072.1"
                     /translation="MGKTHKKTHRRQGSIKRARHRDRRMKIGAIVLCAAIIITTFLAC
                     GMQLLVPVADAVVGADNKTQSRDVDVQKNNNSLTASKMDSEKKQNSSEADVNSAEQDS
                     EPDKETKLDEILHSAKYPGQLKELAKKNDEAIDFIYEYPKEHSIEHDIDLTAEASQDT
                     VPLLQQWDKRWGYEKYSGNYFAASGCGPTALSMVVLYLTHDAQASPLAVAEYAKEAGY
                     SVDGSGSAWNLISKGCRHYGVNAKTIKVDEDTFKERLDEGNLIVVNVGPGDFTDNGHF
                     MVITGYDDEGFTINDPNSIIKSNTHWQFERLGSQIRAAWRMFV"
     gene            complement(1609..>2601)
                     /locus_tag="DHV81_00715"
     CDS             complement(1609..>2601)
                     /locus_tag="DHV81_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCI93073.1"
                     /translation="PKDYHSELNLHDTQIAIKTVKDFFQGLLALRLNLSRVSAPLFVD
                     PLSGLNDNLNGVERPVTFDIKEQNGRTAEVVQSLAKWKRYALKKYGFSYGEGLYTDMN
                     AIRRDEITDNIHSIFVDQWDWEKVIKQEERTLDTLKETVNTVYKCLRKTEQYMAIQYD
                     YIDLILPKDITFITTSELEEMFPDNTPKEREYYFAKAKGAICVMQIGDKLANGEPHDG
                     RAPDYDDWALNADIIVYYPVLDIALELSSMGIRVDEKALKEQLDKAGCPERAKLPFQK
                     AILNKELPYTIGGGIGQSRICMFFLRKAHIGEVQSSLWPEETIKACEEHGIQLL"
     assembly_gap    2602..2693
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          854 a          599 c          767 g          784 t
ORIGIN      
        1 ggagaccata tgtccatgta tgctgtgaat gcatcggttc caaagactct ggtgcgccat
       61 ctggtaaaat actatgcaga tacctgcggc agcgatttgc tggaaaggac acttcttgat
      121 gtgcttgata cacctgtttc tcctgagctt ttaccacctg aaaacggtaa gatttcacag
      181 aagacagagg atcttgtggg cccttatgag ctgcatgact tcttccttta caatatgctg
      241 cgctgcggct atgctcctgc caaggtttac cgccttgcaa ggatagcatt tgaaggaaaa
      301 tatgatgatg agtttattct caaatggctt aagaacttct atcgcagatt ttttgcacag
      361 cagttcaagc gctcatgtct gccggatgga ccaaaggtag gaacagtggc ggtctcaccg
      421 agaggagacc taaggatgcc ttctgatgcg tgtggacgta tctggatgga tgaggttgat
      481 aagctgtaat agtaagagga gaacaatggg aaagacacat aagaaaactc atagaaggca
      541 gggaagcata aaacgtgcaa ggcaccgcga caggcgcatg aaaataggcg caatagttct
      601 ttgtgcagcg ataatcataa caacatttct ggcgtgtggc atgcagcttt tagttcccgt
      661 agctgacgcg gtggtaggtg ctgataataa gacgcaatca agagatgttg atgtgcagaa
      721 gaataataat tcacttaccg catctaaaat ggattcagaa aaaaagcaaa acagctcaga
      781 ggctgatgta aactctgcgg agcaagacag tgagcctgac aaggaaacga agcttgatga
      841 gatacttcac agtgcaaagt atccgggaca gctaaaggag cttgccaaga aaaatgatga
      901 ggcaattgac ttcatatatg agtatcctaa ggagcattcg atagagcatg atattgatct
      961 gactgcagag gcttcgcagg atacagtacc tcttttgcag cagtgggata agagatgggg
     1021 ctatgaaaag tacagtggca attattttgc ggcgagtggc tgcggtccga ctgcactttc
     1081 gatggttgta ttatatctga cacatgatgc acaggcatcc cctcttgcgg tggcagagta
     1141 tgcaaaggag gccggctaca gtgtggatgg ctccggaagt gcatggaatc ttataagcaa
     1201 gggctgcagg cactacgggg taaatgcaaa gactataaag gtggatgagg atacatttaa
     1261 agaaaggctt gatgagggca atctgatagt cgtaaatgtc ggtccgggag atttcacaga
     1321 caatggacat tttatggtga tcacaggcta tgatgatgag ggctttacta taaatgatcc
     1381 aaacagtatc ataaagagca atacccactg gcagtttgag aggctgggtt cgcagatcag
     1441 ggctgcatgg aggatgtttg tgtagataat agcagcaata catgcattta tgctgatgtt
     1501 tgtgatgcat atggaaaaat taaaaaataa gaaataaaaa acctgctgtg tcatgcttgt
     1561 ctgacacagc aggtttttaa taacaaacag taaagtatgg tatgcagatt atagcaattg
     1621 aataccatgc tcctcacatg ccttaattgt ctcctcaggc cacagtgagc tctgtacctc
     1681 accgatatga gcctttctga ggaagaacat acagattcgt gactgtccga ttccgcctcc
     1741 gatagtgtac ggaagctcct tgttgaggat agccttctgg aatggaagct tagcacgctc
     1801 aggacatccg gccttgtcaa gctgctcctt tagagctttt tcatctacac gtatacccat
     1861 tgatgaaagc tcaagtgcga tatcaagaac aggatagtat acgatgatat cagcgtttaa
     1921 agcccagtcg tcatagtcag gggcacgtcc gtcgtgaggc tctccgtttg caagcttgtc
     1981 accaatctgc attacacaga tggcaccctt tgcctttgca aaataatact cgcgctcctt
     2041 tggagtattg tccgggaaca tttcctcaag ctctgatgtg gtgataaagg tgatatcctt
     2101 tggcagaatc aaatctatgt agtcgtactg tatagccatg tactgctcgg tcttgcgcag
     2161 gcatttgtat acggtgttca ccgtttcctt taaggtgtcg agcgtgcgct cctcctgctt
     2221 gatgactttc tcccaatccc actgatccac gaagatggag tggatattgt cggtgatctc
     2281 atcgcgccgg atggcgttca tgtcggtgta aagaccttcc ccgtaagaaa agccgtattt
     2341 tttgagcgcg tagcgcttcc atttggcaag tgactgaaca acctccgcgg ttctgccgtt
     2401 ctgctccttg atgtcgaagg tgactgggcg ctccacaccg tttaagttgt cgttgagacc
     2461 ggacagcggg tctacaaaca gaggggcgga tacacgggac agattcaggc gcagtgcaag
     2521 cagtccctga aaaaagtcct tgaccgtctt gatcgcgatc tgtgtatcat gcaaattcaa
     2581 ttctgaatga taatcttttg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntaaccta
     2701 aataattata gcacagggca gttcattttc aagaaaagat acaggtattt gaaaaataat
     2761 acgtgtatac aatttgagac gaaagggcgc tcggaaatgg tataaacatg cgacttcagg
     2821 gcgggaatcc gtcgatagat gttgacagta atatgcaaaa atgagaaaat attggcaaga
     2881 ttattatgca attatcatag aatctggggt atcacaagag aaaaggagtt gaggcacatg
     2941 caaaaggaaa aaggaatcgt atcgattaag gttaagcttt taggtgtgat cgttccggtt
     3001 gtcattgtca gcattctggt gctcgtactg atcgcctacc gcacgtcggc aggattgatt
     3061 gagagctatt ccgagaatct gctggagtca tcggtg
//
LOCUS       DPUF01000023           14903 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8340, whole
            genome shotgun sequence.
ACCESSION   DPUF01000023 DPUF01000000
VERSION     DPUF01000023.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 14903)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14903)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14903
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8340"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="DHV81_00720"
     CDS             <1..444
                     /locus_tag="DHV81_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCI93074.1"
                     /translation="TLDGGRIGIAAQALGIAEGALERTIEYTKERKQFGRSIAQQQNT
                     QFKLADMATRIDAAKYLVYAAAMKKAEFAKNPKVSYSVDAAKAKLFAAETAMAVTTEC
                     VQLFGGYGYIREYDVERMMRDAKITEIYEGTSEVQRMVISGSLLR"
     gene            502..1302
                     /locus_tag="DHV81_00725"
                     /pseudo
     CDS             502..1302
                     /locus_tag="DHV81_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741638.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
     assembly_gap    1068..1152
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1319..2365
                     /locus_tag="DHV81_00730"
     CDS             1319..2365
                     /locus_tag="DHV81_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCI93075.1"
                     /translation="MGLEEYKGVYIYAQQVDNKLSDIAFELVGKAKELAADLNTEVTA
                     VLLGSNVKALATELGEYGADKVIVVDNPALETYRTEPYAQALVSVINEYKPEIMLVGA
                     TAIGRDLGPTVSARVKTGLTADCTKLEIGDFPINAMPGQEQKHNQLLMTRPAFGGNTI
                     ATIACPDNRPQMATVRPGVMQKLPKEAGRAAEVIEYNPALEENNRYVEIMDIVKAVGN
                     VENIMDAKVLVSGGRGVGSAENFEMLRDLASCFKGGMVSCSRAAVENGWLAQDYQVGQ
                     TGKTVRPQIYFAIGISGAIQHVAGMEESDLIIAINKDEDAPIFDVADYGLVGDLKKIV
                     PQLTAALKEANADA"
     gene            complement(2556..2774)
                     /locus_tag="DHV81_00735"
     CDS             complement(2556..2774)
                     /locus_tag="DHV81_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCI93076.1"
                     /translation="MDYIQRMRNLREDSDKTQQQIADYLGTSQTMYARYERGANELPI
                     HHLLALCKYYNVSADYILGLSNIKQISK"
     gene            2904..3194
                     /locus_tag="DHV81_00740"
     CDS             2904..3194
                     /locus_tag="DHV81_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCI93077.1"
                     /translation="MALIRCPECGKEISDSAQVCPNCGCKTELGNQNEIMKTSLLADI
                     GFTVLGIICAIFAFVAYYTVSTDAFVVLIILAAVLFIIGSVYGRLIRKNNKK"
     gene            3214..3702
                     /locus_tag="DHV81_00745"
     CDS             3214..3702
                     /locus_tag="DHV81_00745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93078.1"
                     /translation="MNKKIITIIFTLSTMLFLVGCSFTNNESNGSDKNYTYNDLDENQ
                     KEIIDNVYAELGDWGYTYEPSAIPASKIKFFYEDSKLIFAAFHDYGGGNGGGSYSVYE
                     IDENSGTVSGHSYDTLNENDVLNKRVLAVELLSGESFDVEASEDSQKDILANSYGKAV
                     NE"
     gene            4088..4663
                     /locus_tag="DHV81_00750"
     CDS             4088..4663
                     /locus_tag="DHV81_00750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93079.1"
                     /translation="MKKIKLYTILMVSVLLTACGTDKKIEKQANNIVDAVENNDISEL
                     DFIINGTDELAEDEELADFFETDTEQGNGLMSKIIKHDTIEVKKVGKDAITYKITAPD
                     LSNLFSDMKAGNVTEENIDSFMDDYISDAKMVTNEVDVSYTYENEKFSAEYDTEEFIN
                     ALTGNMVTAYQELIQDVQNEISEEDNNEESN"
     gene            4647..7568
                     /locus_tag="DHV81_00755"
     CDS             4647..7568
                     /locus_tag="DHV81_00755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93080.1"
                     /translation="MKRVISFFLCAIMLMSSAHTVLATEITDISYNTLSVEFSDNLGT
                     TENLKAMVTEDHFYVDAEQLGDRLGYQVEVGNEYVAIYNKSFSNTVPYGLTAFYYDST
                     KVRHMLFNKMVDYEAPFKIVKNSDGVWIPFEFSLLLLNSSDVVLDNQVHIEMPQKNII
                     DIYMDILKNNDRYLFDWKSDAGASDGSIFAMGTGAYFVQVFSGILDKDGASWLQLIDS
                     FNLKMDSYDRKYAESFARMFCTYSDEELTLNVEAMKEKMKPFNGDNWVTKSIKTVDDI
                     LDDNIGDLSEKTAALKKKIVVENGTSVAKFNKSYQELDKMCNTSDFFSDVTDPFFMAS
                     EKFKDATSFLDVFYKISEVVGYASEFKNQDEFAVKSLDTFIKNADSSCVMSKAMKDSM
                     EEYKKTLETDIVAYSAYNYLMNNMTDLFTDTIDISTTILDMDSKLYLLAWDIAKGAIP
                     LVKDSLSNTDCFLQSMYAGVVQSDTFCAYINRRNDVFGDAKNITPENLYELSQYCYAY
                     LKSCYITRDAAVGSLTEKTKKNNPTYASTQKMVNQEIAEYLIKLKNADKTNKYGCYGF
                     LPENNEQYLGEYDDSSLINCLGTSKDGEELYKDILDMYYYKIKNQNWTENDNVSILLS
                     GNYSDYKSLSSVGYALLDIDGNGIPELLIGDNDSQSDGVIIDLYTYVDDKVVYLDTTY
                     ERFGINLSKNGKIYRYGSGGAYNNFEEECKIDEKNKVLVPIESIVFDGYYNPNSPWYY
                     ATGNYYTDSYDYDLDKMKNISEEEAMNKRKEFENNIIGYSITLFSDYIPQSDNDNISS
                     NAGVYDEFLKNREYKNYIADSWTFGIPKQYSVMDIDGDGTDELLIYGSEDLWEDLAIF
                     SFNSNTQQISLIPAMNYDSDEPQQISVLSAYGKIKYSEKYHAIVYSQYQNYSNGIACD
                     SISYDVIKNGILDNDFIIGFGINSDTSEKEYYCGTENLTESEYNEYIAECSEISWMDL
                     P"
     gene            7816..8004
                     /locus_tag="DHV81_00760"
     CDS             7816..8004
                     /locus_tag="DHV81_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HCI93081.1"
                     /translation="MNNNMEYKGYLGSVEFSEEEAVFFGKIMNIRSLISYEGKNVMEL
                     SEQFREAVDEYSSICEQQ"
     gene            8064..8259
                     /locus_tag="DHV81_00765"
                     /pseudo
     CDS             8064..8259
                     /locus_tag="DHV81_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743948.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8381..9331
                     /locus_tag="DHV81_00770"
                     /pseudo
     CDS             8381..9331
                     /locus_tag="DHV81_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9797..11611
                     /gene="glmS"
                     /locus_tag="DHV81_00775"
                     /pseudo
     CDS             9797..11611
                     /gene="glmS"
                     /locus_tag="DHV81_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741712.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
     assembly_gap    9997..10081
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11711..11822
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11902..12720
                     /locus_tag="DHV81_00780"
     CDS             11902..12720
                     /locus_tag="DHV81_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCI93082.1"
                     /translation="MRNSKTNELVLTGLFTAIIIIMAFTPLGYIPLVVINATIIHIPV
                     ILGSLFCGPKKGAFLGFVFGFTSCLKATIVGGTLSSFVFSPVLAASLVGTSGIFKSLF
                     IAFVPRILVGVIPYFVYIGIKKVLASEKKNLWGTVLNVLMSVFLAFGVYAFLGKMSES
                     LSQAAALGIGIAVGAVVFIVVEAVFIRKSTQVIPFVYAGVAGAMTNTLLVMGSIFVLY
                     KDAYADAIGVAGNAVLGVITGVISFNGVIEAIVAAIIVYLVGLVLNTIKPIAAK"
     gene            12858..13823
                     /locus_tag="DHV81_00785"
     CDS             12858..13823
                     /locus_tag="DHV81_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCI93083.1"
                     /translation="MHEIDIMDIGGFKIGQAEDTKGLTGCTVFLFDKQSPAGVDIRGG
                     GPASRETPLLNPVADAKGIHALVLSGGSAFGLDAAGGVMKYLEERDIGFDVGVTKVPL
                     VVQSCIFDLVIGDKNVRPDGKMAYKACENASYDTFLQGNYGAGMGATVGKYMGRERSM
                     KSGIGAYAVQLGELKVGAVVTLNALGDIYDIDTDKKIAGALDENGLLFDDETAYFEAA
                     AKIQNMFTGNTTIGTIITNAKLDKTALGKVASMAHNGYGRAIRPVHTMADGDSIYAVS
                     VGSVPADINLVGPMSAYVMAKAIANAARSAKSVDGYKAFCDVNKK"
     gene            13862..>14298
                     /locus_tag="DHV81_00790"
     CDS             13862..>14298
                     /locus_tag="DHV81_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCI93084.1"
                     /translation="MLKGKTVVLGVTGSIAAYKIANLASMLVKQHADVNVIMTHNATN
                     FINPITFETLTGNKCLVDTFDRNFEFNVEHVALAKRADIFMVAPASANVIGKMANGIA
                     DDMLTTTILAAKCPKLVSPAMNTNMYENRIVQDNIKKLEHYGF"
     assembly_gap    14299..14409
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14410..>14903
                     /locus_tag="DHV81_00795"
     CDS             <14410..>14903
                     /locus_tag="DHV81_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCI93085.1"
                     /translation="LITAGATQEKIDPVRFITNHSTGKMGRAIAENCMLRGAHVTLVN
                     ASVSVEPPMFVDVVNVTSAADMADVVKSKAAEQDIIIMTAAVADFCPSHPADEKIKKN
                     DSSYESVIELERTEDILSYLGAHKAKGQLICGFSMETQNMLENSKAKLAKKNADMIVA
                     NNLKV"
BASE COUNT         4886 a         2293 c         3239 g         4092 t
ORIGIN      
        1 acacttgatg gtggacgtat cggaatcgct gctcaggcac ttggtattgc agagggcgca
       61 ttagagagaa ctatcgagta cacaaaggaa agaaagcagt tcggccgttc aatcgcacag
      121 cagcagaata cacagttcaa gcttgcagat atggctacac gtatcgatgc agctaagtat
      181 cttgtatacg cagcagctat gaagaaggca gagtttgcta agaaccctaa ggtttcttac
      241 tcagtagatg cagcaaaggc taaattattc gcagcagaga cagctatggc tgttacaaca
      301 gagtgcgttc agttattcgg tggatacgga tatatccgtg agtacgatgt agagcgtatg
      361 atgcgtgatg ctaagattac agagatctac gagggaactt cagaggttca gcgtatggtt
      421 atttcaggct cattactcag atagttaaga gagctatcat aactgtagag ctacttagaa
      481 taaataaagg agaacaaata catgaatatc gtagtatgta ttaaacaggt tcctgataca
      541 aagggtggag taaaattcaa tcctgacgga acacttgata gagcttcaat gcttgcaatc
      601 atgaatccag atgacaaggc tggtcttgag gcagcactta gaatcaaaga cgagacaggc
      661 gcaaaggtta cagtccttac aatgggactt ccaaaggctg atgcaatcct tcgtgaggca
      721 atggctatgg gcgctgatga tgcagtttta gtaacagaca gagtacttgg tggagctgat
      781 acatgggcta catctactac aattgcaggt gcattaagaa atctcgatta tgatttaatc
      841 atcacaggcc gtcaggctat cgatggagat acagcacagg ttggaccaca gatcgcagtt
      901 catcttggac ttcctgtaat ctcatacgca gaggatatca aggttgaggg agattctgta
      961 gtcgttaagc gtcagtacga ggacagatat cacgtagtta aggctaagat gccttgtctt
     1021 atcacagctc tttcagagtt aaatgagcct agatatatga caccgggnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nngattcaga tcttggactt aagggatcac ctacaaagat tgctaaggca
     1201 tctgacaagg tacctaaggg agctggagaa aaggttaacc ttgatccaac agagtcagta
     1261 aactatctca tggagaaatt caaagagaaa catatcatct agtatggagg aaccaaaaat
     1321 gggtttagaa gaatataagg gcgtatatat ctacgcacag caggtagaca ataagcttag
     1381 cgacatcgcc tttgagttag tcggtaaagc taaagagtta gcagcagatt taaatacaga
     1441 ggttactgca gtacttcttg gatctaacgt aaaggctctt gcaacagagc tcggtgagta
     1501 cggcgcagac aaggtaatcg ttgtagacaa tcctgcactt gagacataca gaacagagcc
     1561 atacgcacag gctcttgtat cagtaatcaa cgagtacaag ccggagatca tgttagttgg
     1621 tgcaacagct atcggacgtg acctcggacc aacagtttca gcaagagtta agacaggtct
     1681 tacagctgac tgtacaaagc ttgagattgg tgatttccca atcaatgcta tgcctggaca
     1741 ggagcagaag cacaaccagc ttctcatgac acgtcctgca ttcggtggaa acacaatcgc
     1801 aacaatcgct tgtcctgaca accgtccaca gatggcaaca gttcgtcctg gtgttatgca
     1861 gaagcttcct aaagaggctg gcagagcagc agaggttatc gagtacaatc ctgcactcga
     1921 ggagaacaac cgttatgttg agatcatgga tatcgttaag gcagttggca acgtagagaa
     1981 catcatggac gctaaggtac ttgtatcagg cggacgtgga gttggaagcg ctgagaactt
     2041 cgagatgctt cgtgaccttg cttcatgctt caagggtggt atggtatcat gctctcgtgc
     2101 agcagtagag aacggctggt tagcacagga ttatcaggta ggacagacag gaaagactgt
     2161 acgtccacag atttacttcg caatcggtat ctcaggagcc atccagcacg tagcaggtat
     2221 ggaggagtct gatctcatca tcgccatcaa caaggacgag gatgctccaa tcttcgatgt
     2281 agctgattac ggcttagtag gagatttaaa gaagatcgtt cctcagctta cagcagctct
     2341 taaagaggca aatgctgacg cataatcgca taataatgca ataaaattac attactaata
     2401 catttaaaca cagtactgtt tatcgcagtg ctgtgttttt tttgtgcata aatatagcgt
     2461 aatcttataa atataaagag ttttttgggg aaataagtaa ttttcttaat aaaaaacatc
     2521 cgtgtgtaaa tcgcaaagga tttgactcgg atgttttatt tggaaatttg tttgatatta
     2581 gaaagcccta atatatagtc agcggacaca ttatagtatt tacataaagc aagcaaatgg
     2641 tgaatcggta gttcatttgc tccgcgctca tatctggcat acattgtctg ggatgtaccc
     2701 agataatcgg ctatttgctg ttgggtttta tcgctatctt cccgtagatt gcgcattcgt
     2761 tgaatgtaat ccatattccc cctccgtaca tgtacatata acttaacata ataaagatgt
     2821 ttactattga atttagtaaa tatttgtact ataatttatg taggaaagat gtcacaggat
     2881 tataaagaaa ggtaggtatt aatatggctt taataagatg tccagaatgt ggaaaagaaa
     2941 taagtgattc ggcacaagtg tgtccaaatt gtggctgtaa gacagagttg ggaaatcaaa
     3001 atgaaattat gaaaacttct ttactcgcgg atataggttt tacagtttta ggaataatat
     3061 gtgctatatt tgcatttgta gcttactata cagttagtac ggatgcattt gtagtgttga
     3121 ttattttggc agctgtttta tttattattg gctcagtcta tggaagatta ataagaaaaa
     3181 ataataagaa ataatatctt tgagaggcgt aagatgaata agaagataat aactataata
     3241 tttactctaa gtactatgct ttttctagtt ggatgttctt ttacaaacaa tgaatcaaat
     3301 gggagtgata aaaattatac ctataatgat ttagacgaga atcaaaaaga aatcatagat
     3361 aatgtgtatg ctgaattagg tgattgggga tatacgtatg agccaagtgc tattccggcg
     3421 agtaagataa agttttttta tgaggattcg aaattgatat ttgcggcttt tcatgattat
     3481 gggggtggaa atggtggagg ctcttattca gtatatgaaa tagatgagaa ttctggaact
     3541 gtaagtggac attcatatga cacgctaaac gaaaatgatg tactaaataa acgagtttta
     3601 gctgtggaac ttttatcggg agaaagtttt gatgttgaag cttccgaaga ctcgcaaaaa
     3661 gatattttgg caaattcata tggtaaagct gtaaatgagt aataatatga ctataacaga
     3721 attggagaaa attaaaaatg ggctattgcg gtaattgtgg tgaaaaatag aaaatggagc
     3781 taatttttgc ggtaaatgtg gtgcggttgt tgagaaagaa caatcagaac atgtttatga
     3841 tgttatagca ccaaagaaca atagcgctag agtatggaaa attataatcc ctaaatatat
     3901 ttgatgatac cgttatatct ggtactgatg cattatatat agctagacaa aatggcgata
     3961 aatatgatat ttatagattt gcagcaaaaa aataaaataa ttagatatag ataaatatca
     4021 aaaaagtgat tcatgatagg tgtaatgttc tgtgaatcac ttttttacaa ataaaggaat
     4081 agataagatg aagaaaataa aactttacac aattttgatg gtttctgtat tgcttacagc
     4141 ctgtggcaca gataaaaaaa tcgaaaaaca ggcaaataac atagtggatg cagttgaaaa
     4201 taatgatata tcagaactgg attttatcat taatggaact gatgaacttg cagaggatga
     4261 agaacttgca gatttctttg agacagatac cgaacaagga aatggtctga tgtctaaaat
     4321 aataaagcac gatactattg aagtcaaaaa ggtaggtaag gatgctatta cctacaaaat
     4381 cacagctcca gacttatcga atttattttc tgatatgaaa gctggtaatg ttactgaaga
     4441 aaatatagat tcttttatgg atgactatat atccgatgcc aagatggtta ctaatgaagt
     4501 ggatgtttcg tatacatatg agaatgaaaa attttcggca gaatatgata cagaagaatt
     4561 tattaatgca ttgacaggaa atatggttac agcgtatcag gaactgattc aggatgtgca
     4621 gaatgagata agtgaggagg ataataatga agagagtaat tagttttttt ctttgtgcaa
     4681 ttatgctgat gtcatctgca catacggtat tggctacaga aataacagat atatcttata
     4741 atactctttc tgttgagttt tctgataacc ttggaacaac agaaaatctt aaggctatgg
     4801 tgacagaaga tcacttttat gtagatgcag aacagttggg agacagactt gggtatcagg
     4861 ttgaagttgg aaatgaatat gtagcaattt ataataaaag ttttagtaat acagttccat
     4921 atggtcttac tgctttttac tatgacagta caaaagtaag acatatgtta tttaataaaa
     4981 tggttgatta tgaagctcca tttaagattg ttaaaaattc ggacggtgtg tggattccat
     5041 ttgaatttag tcttttactg ttaaatagtt ctgatgttgt gctggataat caggtacaca
     5101 ttgaaatgcc acaaaagaat atcatagata tctatatgga tatattaaaa aataacgata
     5161 gatatttatt tgattggaaa agtgatgccg gggcttctga tggctcaata tttgccatgg
     5221 ggacaggggc gtattttgtg caggtattta gtggtattct ggataaagat ggagcatcat
     5281 ggttacaatt aatagatagc tttaatctta aaatggatag ctatgaccga aaatatgcag
     5341 aaagctttgc cagaatgttt tgtacatact cagatgaaga attaactctt aatgtagaag
     5401 caatgaaaga gaaaatgaaa ccttttaatg gggataactg ggttacaaaa tcaataaaaa
     5461 cagttgacga tattttagat gataatattg gagatttatc tgaaaaaaca gcagctttaa
     5521 agaaaaagat tgttgtagaa aatggcacat ctgttgcgaa atttaacaaa tcatatcagg
     5581 aacttgacaa aatgtgtaat acatcagatt ttttctctga tgtgacagat ccatttttta
     5641 tggcttcaga gaaatttaag gatgcaacca gttttttgga tgtgttttat aagatatcag
     5701 aagttgttgg atatgccagt gaatttaaaa atcaggatga atttgcagta aaatctttag
     5761 atacatttat caaaaatgct gattcaagct gtgttatgtc taaggctatg aaagatagca
     5821 tggaagaata caaaaagacg ctggaaacag atattgttgc atattctgcg tacaattatc
     5881 tgatgaataa catgacagac ttatttacag ataccataga tatatcaact acaatactgg
     5941 atatggattc taagctgtat ttgttagcat gggatattgc taaaggagca attcctcttg
     6001 tcaaagatag tctaagtaat acggactgtt ttttacagag tatgtatgcc ggagtcgtac
     6061 agtcagacac tttttgtgca tatataaaca gacgcaatga tgtgtttgga gatgctaaga
     6121 acataacacc ggaaaatttg tatgaacttt cacaatattg ttatgcatat cttaaaagct
     6181 gttatataac aagggatgca gcagtaggtt cattaactga aaaaacaaag aaaaataatc
     6241 ctacatatgc aagtactcag aagatggtaa atcaggaaat tgcagagtat ttgattaagc
     6301 taaaaaatgc ggataaaaca aataagtatg gatgctatgg attcctgccg gaaaacaatg
     6361 aacagtattt gggcgagtat gatgatagtt ctttgataaa ttgcctagga acatctaaag
     6421 atggggaaga attatacaaa gatattttag atatgtacta ttataagatt aaaaatcaga
     6481 attggacgga aaatgataac gtcagtattt tgttgagtgg gaattattcg gactataaat
     6541 ctttaagtag cgtagggtat gcacttttgg atatcgatgg gaatggaata ccggaattac
     6601 ttattggaga caatgactca caatcagatg gagtaatcat tgatttgtat acatacgtag
     6661 atgataaagt tgtctatctt gatacaacat atgaaagatt tggaattaac ttatctaaaa
     6721 acggaaagat atacagatat ggttctggtg gggcgtataa taattttgaa gaagagtgta
     6781 aaatagacga gaaaaataaa gtactagtac caattgagtc gattgttttt gatggatatt
     6841 ataatccaaa ttcgccatgg tattatgcta caggtaatta ttatacagat tcatatgatt
     6901 atgatttaga caaaatgaaa aacatatcag aagaagaggc tatgaataaa agaaaagaat
     6961 tcgaaaataa tattattggc tattcaatta cactcttttc agattatatt ccgcaatcag
     7021 ataatgataa tatttcatct aatgctggag tatatgacga gtttcttaaa aatagagaat
     7081 acaaaaatta tatagcggat tcttggacat ttggcattcc aaaacaatat tcagttatgg
     7141 atatagatgg agatggaaca gatgaattat taatatacgg aagtgaagat ttatgggagg
     7201 atttagctat tttttccttc aattcaaata cacaacaaat ttcattaata ccggcaatga
     7261 attatgattc agatgaacca caacaaatta gtgtgctatc tgcctatgga aaaattaaat
     7321 actctgaaaa atatcatgca attgtatata gccaatatca aaactatagt aatggtattg
     7381 cttgcgattc aatttcatat gatgttatta agaatggtat tttagataat gattttataa
     7441 taggttttgg aattaattct gacacaagtg aaaaggagta ttattgtggc acagagaact
     7501 taacagaatc agaatataat gaatatatag cagagtgctc ggaaatatct tggatggatt
     7561 taccataaaa gtatagaaca tagttactat taatgatttg catacttata ctaaagagta
     7621 aatattttca agttttgata gcgaagtatt atacaataga taagtattaa agaaaatatc
     7681 ccctacaatt gatctcatat attatgatgg caaaagtaga ggatattttt tttatacact
     7741 actaacattt ttgatgtacg aaaagaatac atataagcct ttgataagaa tgtcatagga
     7801 aatggagaat cgaacatgaa taacaatatg gaatataaag gatatttagg aagtgttgaa
     7861 ttttcagaag aggaagctgt tttcttcgga aaaataatga atatacgttc attgatatcg
     7921 tatgagggta aaaatgtaat ggaactttca gaacaatttc gtgaagctgt agatgagtat
     7981 tcatcaatat gtgagcaaca atgaaactct ttagtaaaag tgcagaaatg atatatttaa
     8041 atgtaaaata ataaaaatta aagatgacaa aacgattgcc agatgttaca atcatttcaa
     8101 agcattatat ttctatttat atggcaccac attacagatt tacataggtg catatcactt
     8161 tgaagagggc agaccacttc gcattcattc tacaaagaca tggaaaattt tcaatgcttt
     8221 atgttatcaa ttaatagcag gaaaatggat atgtcagatg caaatgacgc tgtaggagta
     8281 aatgtatttg tggggaagaa gcctttagat aatgatatag ctgcctttgt gattgatata
     8341 atccaattat ctgctcacat aactgaatag gaggagaaaa ttgggtacaa aagactcaaa
     8401 agcaaaagag tattttgcag acaatgagcg gtttgcagat ttttgcaact atgtattgta
     8461 tggtggcagg tgtgttatca aagctgaaaa tctcgaggag agagatacta cggaggtact
     8521 gacagcatta ggactcagtc ttaatatgat atcagtgcaa aagtggagag atatttttaa
     8581 aaatatccat gttaaatata cgggaaagac atatatcttt cttgtgggga tggaaaatca
     8641 atcagatatt cattatgcta tgcctgtcaa aaacatgatc tatgatgcac ttgcgtatgg
     8701 caaacaagta agagaagtag cgaagaaaca tcgtcgtgag catgataccg aaacaccaga
     8761 tgaatttctt tccggattta ccgcaaatga caggttgata ccggtaatta ccattactgt
     8821 ttagttatgt gtaaaagaat ggaatggacc tagaaaattg actgatatgc ttgaagacat
     8881 tgatgaagaa ttattatcat tcatttcgga ttacaagatt aatttactgg agcctaagag
     8941 cataatggat ttcacaaaat tcaggacaca gcttaaacag ctatttgagg tattgcagaa
     9001 tgcctcggat aagaatagat tacaggctgt tttgcaggaa gatgaacaat ttaagaatat
     9061 ggacagagaa actgtagagg caataaattt atttgcaggt atgaatatac aaaccgatgg
     9121 caaggaagag gtgattgata tgtgtaaagc atgggaagaa cagtgagaag aaggtattga
     9181 gcagggcatt gaacagggaa gaaaaaccga agtttttgac tcggtgcagt gtggagacta
     9241 tagtactgca agaggtgcac agaaattaaa tctgtatatt gatgaattta agaaacagat
     9301 gatggcagcg ggattttcaa taccgcaata aaacacggtc cggcaaatat gccggaccat
     9361 tttgttacat tgagtatcga taatatcaat aattgatccg gagaatagat tttgattata
     9421 atccgatata tagtcgcata ttatatattt accccaacat ttacgaaaat ttcagcactt
     9481 tttactgttg taggtacttt tggtttatga tagaataaca ccgtccgatg aaagtggagg
     9541 aattggattt cgaaaagtta aaaactttag cgccatgcac cggcaggtac gtaaaaatga
     9601 tgccggttaa cgaggtttca ggttatcgaa aattcggcgg atgcctggac gcatatatgg
     9661 atgcgcatct caagccaaat atgatggaga catcaaaaca aagcgagaga accataaaaa
     9721 tcaagtagct attcagtaca gaacagttac gtatttgtac atttaaaatt atatatcgaa
     9781 aaggagatct aacactatgt gtggaattat aggatttaca ggccgtttag aggcacagaa
     9841 gattttgacg gaaggactcg cagcgttaga gtatcgtgga tacgacagtg caggtattgc
     9901 ttatttcaag gatacaggca agatttctat tcgcaaaacc gttggaaagg ttaaggatct
     9961 tctcgctatc tgcgatgatg agaacaattc aacctgnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 natccacaat ggtatcattg agaattacca cgagatcgta aacaagtttg atcttgcaga
    10141 ctctcttata tctgagacag atacagaggt agcagcggct cttatcaaca agctctatga
    10201 cggagatcca aaggctacta ttaagaaggt agtagcagag cttcagggtt catttgcgtt
    10261 ctgtatcatg ttcaaggatc agccgggaaa gatttttgct gtcagaaatg taagcccgat
    10321 ggttgcaaca tattgcgatg acggagcatt tattgcatca gatcttacag cgtttatcaa
    10381 atattcaaag cgctatttta tacttccgga gtacactatc atgacaatga ctgctgatgg
    10441 aatcgaaatg gaggatttag agggtaagaa agttgagccg gattatcttg aggtaaactg
    10501 ggatgtaacg gcagctcaga aggatggata tccgcatttc atgatcaagg aaattcatga
    10561 gcagccgaca gctatcacaa ggactatcac tccaagaatc aaggattcac ttccttgctt
    10621 tgaggatgat aatattcctg attcgttctt cgaggatata agcgacataa cagttgtagc
    10681 ctgtggtaca gccatgtatg caggaatggt cggaaaagct cttttaaaga acaagtttgg
    10741 tattcctgtt tctgttgaga ttgcatcaga gttcagatat gagcagcctg ttctcacaga
    10801 caggtcaatg gttatttttg tgtcacagtc aggtgagaca atcgatacct tagaggcact
    10861 tcgtctggct aataaatata ctaaaaagac gctttctatc gtaaatgtaa agggttcaac
    10921 aatagcccgt gagagcaact atgtacttta tacacacgca ggtcctgaga ttgccgtggc
    10981 aagtaccaag gcatacacag ttcaggtggc aagcttttac ttgattggat gcaagattgg
    11041 tcttgtatcc ggccgtatga ccaaggaaga ggcaaagaca tttattgaga atctgcagga
    11101 ggttccaaat ctcattgagt cagttatcac acaggcacct gagattgaac agcttacgaa
    11161 gagaatgaca aatgctgcaa atgcgtttta cataggacga ggacttgatt atgcattaag
    11221 catggagggc gcactcaagc tcaaagagat ttcatatatt catgctgagg cttatgcagc
    11281 aggcgagtta aagcacggta caatcgcgct catcagcgag ggggttccgg ttatcgcagt
    11341 tgctacacag agccatgtat acagcaaggt catctcaaat atccgcgagg taaaggcgag
    11401 aggcgcatac gttatccttc ttagcaagga taaggatatt acagataagt caatctgtga
    11461 tgtgcatatc tcactcccac aggcaccgga tgagtttgtg attttcgagt cagttataat
    11521 ctgtcagctt atcgcttact acacatcaac aggaaagggc ttaaaccctg accagccacg
    11581 taaccttgca aaatcagtta cagtagaata aataatttcc ccggacattt atacaatgtc
    11641 cggggatttt tttcacgctt gtgtgataca atgcagcata tgtaagacat cgatgtatat
    11701 gtcagtgatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnttatgccg tgtgacagaa tcacagcata acagaattag gacgagaccc atatgggctt
    11881 ttaccaaaag gaggaaaaac aatgcgcaat tcaaaaacaa acgaacttgt actgacaggt
    11941 ttatttacag ccattatcat tatcatggca ttcacaccac tcggatatat tccgctcgta
    12001 gttatcaacg ctacgattat tcacattccg gtaatccttg gttcactttt ctgcggacct
    12061 aaaaagggag cattccttgg atttgtattc ggatttacca gttgtttaaa ggcaactata
    12121 gtcggaggaa ctctttcatc ctttgtattt tcgccggtgc ttgccgcatc cctggtcgga
    12181 acctcaggta tatttaagag cttattcata gcatttgttc caagaatttt agtaggtgtt
    12241 ataccttatt ttgtatatat aggcatcaaa aaggtgcttg cgtcagagaa gaaaaatctc
    12301 tggggcacag ttttaaatgt gctcatgtca gtattccttg cattcggagt atatgcattc
    12361 ctcggcaaga tgtcagagtc actttcacag gctgccgctt taggaatcgg catagcagtc
    12421 ggagctgtag tatttatagt agttgaggct gtatttatca gaaagagcac acaggttatt
    12481 ccatttgtat atgcaggtgt agccggtgcc atgaccaata cactgcttgt aatgggctca
    12541 atcttcgtgc tttacaagga tgcttatgca gatgcaatcg gtgtggcagg aaacgcggtg
    12601 ctgggcgtta tcaccggagt catcagcttt aacggagtaa ttgaggcaat agttgccgca
    12661 atcatcgtat accttgttgg attggtttta aatactatca aaccaattgc agcaaaataa
    12721 attcagatag atttaaaata aatgtgaaat tgtatgtacg gcggactgat aaggtccgcc
    12781 tgttttataa gcagttggac taggaacggt aaaaaagcat ttgagttaaa agaaaggcat
    12841 gatacaaggt gaaacatatg catgaaatag acataatgga tatcggcggc ttcaaaatag
    12901 gccaagccga ggacactaag ggcttgaccg gctgtacggt atttcttttt gataaacagt
    12961 caccggcggg agttgacata aggggaggcg gaccggcttc gcgggagaca ccacttttaa
    13021 atcctgtggc agatgcaaag ggtatccatg cgcttgtact gtcaggcggc tctgcctttg
    13081 gtctggatgc agcgggaggc gtgatgaagt atctcgagga gcgcgatata ggctttgatg
    13141 tgggagtcac aaaggtgcct cttgtggtgc agtcgtgtat ttttgacctg gtaataggtg
    13201 ataaaaatgt aaggccagat ggcaaaatgg catacaaggc ttgtgagaac gcttcttatg
    13261 ataccttttt acagggaaat tatggagccg gaatgggcgc aacagtcggc aaatatatgg
    13321 ggcgtgagcg cagcatgaag agcggaatag gagcctatgc agtacagctt ggagaactaa
    13381 aggttggagc agtggtgaca ctgaacgcac ttggtgatat atatgacata gatacagata
    13441 agaaaattgc cggagcactg gatgaaaatg ggctgctttt tgatgacgag acagcgtatt
    13501 ttgaggcagc ggcgaagatt cagaatatgt ttacaggaaa caccacaatt ggtacaataa
    13561 taacaaatgc aaagcttgac aagaccgcac taggcaaggt ggcatccatg gctcacaatg
    13621 gctatggcag agcaatcaga cccgtgcaca cgatggcgga cggagattca atctatgctg
    13681 tatcagtggg cagtgtgcct gccgatatca atctggtggg gccaatgagt gcctatgtca
    13741 tggcaaaggc cattgccaat gcagctagaa gtgcaaagtc tgtggatggc tacaaagcat
    13801 tttgcgatgt aaacaaaaaa tgactgcaga ataataatag ttttatacag aggtgaaaat
    13861 catgttaaaa ggaaaaacag tagttttggg agtgacagga agcatagcgg catacaagat
    13921 agcaaacctt gcgagtatgc ttgtaaaaca gcacgccgat gtcaacgtca taatgacaca
    13981 caatgcaaca aattttataa atccaatcac atttgaaact ctcacaggca acaagtgcct
    14041 cgtggacaca tttgacagaa actttgaatt caacgtggag catgtggcgc tcgcaaagag
    14101 agcagacatc tttatggtgg cacctgcatc agccaatgtg ataggcaaga tggcaaacgg
    14161 aatcgcagac gatatgctta ccaccacaat acttgcagcc aaatgtccta agcttgtttc
    14221 tcctgccatg aacaccaata tgtacgagaa caggattgtg caggacaata tcaaaaagct
    14281 cgaacactac ggctttgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnc tcatcacagc cggagcgacg caggaaaaaa tagacccggt acgttttatc
    14461 accaatcact caacaggcaa gatggggcgt gccatagcag aaaactgtat gctccgcggt
    14521 gcgcatgtta cactggtcaa cgcttcagta tcggttgagc cacctatgtt tgtagatgtg
    14581 gtaaatgtga catcggccgc agacatggca gacgtggtga aatcaaaggc tgctgaacag
    14641 gatattatca tcatgaccgc agcagtagcg gatttttgcc catcacatcc ggctgatgag
    14701 aaaataaaga aaaatgacag ctcatatgag tctgtaattg agcttgagcg cacagaggac
    14761 atcctttcat acctcggagc acacaaggct aagggacagc tcatatgcgg tttttctatg
    14821 gaaacccaga atatgcttga aaactcgaaa gcaaagcttg caaagaaaaa tgcagatatg
    14881 atagtggcaa acaacctaaa ggt
//
LOCUS       DPUF01000024           11533 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_5477, whole
            genome shotgun sequence.
ACCESSION   DPUF01000024 DPUF01000000
VERSION     DPUF01000024.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11533)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_5477"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="DHV81_00800"
     CDS             complement(<1..317)
                     /locus_tag="DHV81_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93086.1"
                     /translation="MIPICLILFILFIAVITFAIKRADSAQAKVTEEFWEKERKANST
                     LRGDTTDLCYITIPEKFFPLNNDKINDLRDKTLVNLTGMTNTDLKLKYGILNFKKLSE
                     YDD"
     gene            592..2247
                     /locus_tag="DHV81_00805"
     CDS             592..2247
                     /locus_tag="DHV81_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance protein"
                     /protein_id="HCI93087.1"
                     /translation="MKINKKALLLGTMAFVLVAASGMVSAYASGSSGTKKAKVQTITE
                     GVCIGSVDVGGMTKEEAKKAVDAYVDSIKDTSFSLKGANSSFDTTAQEMSVSADTDAA
                     ADEALSVCHEGSLIDRFVESEELKKGNVAVNMYLSVDKQKTAKMIYDKSDELNIKAVD
                     NSLQRNGDSFDFVPGQEGKEVDVVKSVYAINDFLQNSWDGSANEIELVTNTVQPRGSK
                     EELSKIKDNLGGFSTDFSSSAAGRATNVKNACSLINGSVIYPGEQFSVYEAISPITTD
                     NGYQIAGAYENGQVVDSVGGGVCQVATTLYNAVIRAELDIVQRYNHSMIVSYVKPSDD
                     AAIAGTYKDLKFKNNLDNPVYIEGYCSGGVITFNVYGVETRPANREISFRSETISEED
                     PVTQFKFDAGQPVGYFNTEQSAHKGVTARLWKTVTVDGTVQSDEVFNNSKYKSSPKIV
                     TVGTAGASAEVVAQLQAAAAANDEGSVKSIAASAAAAAQAQAAQTQTQDPNAAGQTQG
                     AQTQTTDPNAAGQAQGTQTQTTDQNAAGQAQGTQTQQPAGQTQ"
     gene            2346..3347
                     /locus_tag="DHV81_00810"
     CDS             2346..3347
                     /locus_tag="DHV81_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCI93088.1"
                     /translation="MKNGKISESVLKRSVLKQLHTTSDRILFKPAVGEDCAVISMQAG
                     GQTKLVTATQTFTLDVSDKHVRANCAVYDALNNIYAMGAGPVGIELALLVPTTENEAV
                     LRETVRAIDAVCEEAGIVVLGGHTQVSRAVKNIVVTVTAIGEAYEQALTPSSAAAPGM
                     DVIVTGYVGLEGTAILANEKRAELETRFSKAFIDKSAAYIDHISTAMEAASAIGAGVA
                     AIHDASQGGIFGALWDMAEASGIGLDIDLKKLPIRQDTIEISEFFDINPYKLLSGGSL
                     IIIAADGTRVVRELEKTGQNAVIVGATTDSNDRVLISGEERRFLETAQTDEIYKVFN"
     gene            3375..3857
                     /locus_tag="DHV81_00815"
     CDS             3375..3857
                     /locus_tag="DHV81_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HCI93089.1"
                     /translation="MREKILTFIEKNSRINLKELAIMLGVDEASVANEIAAMEQENVI
                     CGYHTLIDWDKAGLEKVTAMIEVRVTPQRGMGFDKIAERIYNYPEVKSVYLISGGFDL
                     MVTLEGKTLREVSQFVTDKLSTLDQVLSTKTNFILKKYKDHGTIMAAPVKSDERGIMS
                     "
     gene            3857..5035
                     /locus_tag="DHV81_00820"
     CDS             3857..5035
                     /locus_tag="DHV81_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515998.1"
                     /note="catalyzes the transamination of the aromatic amino
                     acid forming a ketoacid; first step in aromatic amino acid
                     degradation in lactococci; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCI93090.1"
                     /translation="MRDPLSSTIKTIQPSGIRKFFDIVSEMKDAISLGVGEPDFDTPW
                     HIRDEGIYSLEKGRTFYTSNAGLKELKVEIARYLDRRYGMTYDYNKEIMVTVGGSEAI
                     DIALRAMLDPGDEVIIPQPSYVSYVPCTVLAGGTPVIVELKEENDFRLTAEELEAVIT
                     PKTKILIMPFPNNPTGAIMEKADLEAIVQVVKEHDLFVISDEIYSELTYVDKHVSIAS
                     FPGMKERTVLINGFSKAYAMTGWRLGYACAPETILKQMLKIHQFAIMCAPTTSQYAAV
                     EAMKNGDEDVAQMREEYNGRRRYVLHRFKEMGLKCFEPFGAFYAFPSIKEFGMTSDEF
                     ATKLLNSKKIAVVPGTAFGECGEGFLRISYAYSLDDLKEALGRIEEFVTELRAGMDNK
                     "
     gene            5057..5560
                     /locus_tag="DHV81_00825"
     CDS             5057..5560
                     /locus_tag="DHV81_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HCI93091.1"
                     /translation="MAKLNIVLHEPEIPANTGNIGRTCVATGTRLHLIEPLGFSLSEK
                     ALKRAGMDYWKDLDVTTYVDWEDFLEKNPGAKIYYATTKGRHVYSDAHFEPDCYIMFG
                     KESAGIPEEILKEHPDECIRIPMIGETRSLNLSNSVAIVLYEALRQNNFDHMRLEGEL
                     HHLSWDD"
     gene            5650..6507
                     /locus_tag="DHV81_00830"
     CDS             5650..6507
                     /locus_tag="DHV81_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCI93092.1"
                     /translation="MGIIKTSKLVHEFIRRDEEGNVESITTALDNVNLDIKEGQFIAI
                     LGHNGSGKSTLAKHINALLAPSDGTIWVDGMDVSKEENVIPVRKSAGMVFQNPDNQII
                     ANVVEEDVGFGPENIGVPTDEIWQRVDYALRAVGMTKYRTHSPNKLSGGQKQRVAIAG
                     VVAMEPKCIVFDEPTAMLDPNGRKEVIATAHELNKKKGVTIILITHYMEEVVGADHVY
                     VMEKGKVVMDGTPREIFSRVDELKEHRLDVPQVTLVADELKKAGLDIPDGILTREELV
                     NALVALKNR"
     gene            6520..7365
                     /locus_tag="DHV81_00835"
     CDS             6520..7365
                     /locus_tag="DHV81_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCI93093.1"
                     /translation="MSIILDHINYCYSADSAYKVQALKDVSLEINDGEFIGIIGHTGS
                     GKSTLIQHMNGLLKATSGHIYYNGQDIYDNDYDLSKLRHKVGMVFQYPEHQLFETTNF
                     EDVCFGPKNQGLDRKTVELRAYEALQNVHFPEDLYYQPPFDLSGGQKRRVAIAGVLAM
                     HPDVLILDEPTAGLDPAGRDEILGLLLQMKKDLGITIILVSHSMEDVAEYVDRIIVMN
                     SGEVMFDGVPKEVFSHYKELEAVGLAAPQVTYLMHDLAEKGIPVDLSATTVAEARDSI
                     LKAFK"
     gene            7415..8248
                     /locus_tag="DHV81_00840"
     CDS             7415..8248
                     /locus_tag="DHV81_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCI93094.1"
                     /translation="MIRDITIGQYYPAQSPIHKLDPRVKLFATLIYIIALFSYKGIAG
                     VLFIVAALAAVIKISKVPFKFMVKGLKSIIILLLFTACFSVFFTQGEYTIWEYGILHI
                     SDTGLINAATMMIRLVLLIIGTSLMTLTTTPNQLTDGLEKSLGFLNKIHVPVHAIAMM
                     MSIALRFIPILIEETDKIMKAQMARGADFEHGNIIKRAKSMVPLLVPLFVSAFRRADD
                     LAMAMEARCYNGGEGRTKMKPLVYEKDDKIAYLFIFAFLAVVIVLRIFLPVNLGNLIF
                     R"
     gene            8284..9018
                     /locus_tag="DHV81_00845"
     CDS             8284..9018
                     /locus_tag="DHV81_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCI93095.1"
                     /translation="MRVKMIVAYDGTNYCGWQIQPNGITIEQVLNEHLSRLLKEDIKV
                     TGASRTDAGVHSLGNVCMFDTNTRMPAEKISYALNQKLPEDIVVVDSCEVSDDFHPRF
                     SKSRKTYEYRILNARFRNPTRRLDTYFYHYPLDTEKMSEAAKYLIGEHDFASFCSANS
                     QAQTTVRTIYDCTVSKAGDIITIRVTGNGFLYNMVRIIAGTLVKIGGSDMPADAMKDI
                     IAATDRAAAGPTAPAHGLTMIGLKYE"
     gene            9146..10381
                     /locus_tag="DHV81_00850"
     CDS             9146..10381
                     /locus_tag="DHV81_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93096.1"
                     /translation="MRFTKVKEKIIKNKPINSTDEGKGRHRKVIRIGAFALVFVVLFS
                     FVSTFFKMTDAVNIATIEGFYKEPKNTIDVMMIGASEVYADYSATEAWKNYGYTSYSL
                     GVSGVPGSLYKSMLREALTRQHPKVVVFEVNGFLQNDSYYDRSVKLHSWIDNIHNEEN
                     RLDTIQEIVPKDEQDNFRNPLKLYHSNWKDIDKCAHTFATRVGMYFAKTSCMKGFSSI
                     AKYSDCPSGKQKKLYFTDKSREYMTDLLEYCKAQGVEKVLFARFPHEKEIKNPQVLCD
                     VKELVESYGYDFASFEGDKEFIGINERDDFYNSEHLNINGSLKFTAYMGKYLSDNYRL
                     NAEHSPELQSAWGECARRADKVISACAKDTDLSLGNHYFEMSVYLPYNFSSSLATNKV
                     ADTNNDAKPAKELNTPVKK"
     gene            10475..11533
                     /locus_tag="DHV81_00855"
                     /pseudo
     CDS             10475..11533
                     /locus_tag="DHV81_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568462.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
     assembly_gap    10810..10892
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3446 a         2211 c         2985 g         2808 t
ORIGIN      
        1 ttatcatcat attcagacag ctttttaaaa ttgagaatgc catatttaag ctttaaatca
       61 gtgtttgtca ttccggtgag attgacaagg gttttatccc ttaaatcatt tattttatca
      121 ttatttaatg gaaaaaactt ttccggtatg gtaatataac acaggtcagt ggtatctccg
      181 cggagggttg agttggcctt gcgctccttt tcccagaact cctcggtgac ctttgcctgg
      241 gcagaatcag ccctctttat ggcaaaagta atcactgcaa taaataaaat gaataaaatt
      301 agacaaattg gaatcatatg ttggaaaatc ctttctcata tatgaaaata gtataactca
      361 tttttgaaca gaggtgaata gtatgtacga ttttaataaa aaaagaaatc agaaggtggt
      421 tgctatagtg ataatagtgg ttatagcagc gatgcttgtg acaacatttg tgtcaagtct
      481 tgtatagctt ttcttgaaat atggaaaaaa tatgataata tgatataaaa tgcagttcag
      541 ttaagcggat gacaggggcg cttttaacag cctcaggaaa aggaatcgga aatgaagatt
      601 aataaaaaag cattattact gggtacaatg gcatttgtgc ttgtggcagc atcaggcatg
      661 gtaagtgcat atgcaagcgg ttcgtcaggc acaaagaagg caaaggtaca gacaattaca
      721 gagggtgtgt gcattggcag tgtggatgta ggcggtatga caaaggaaga agcaaaaaaa
      781 gcagttgatg cctatgttga cagcataaag gatacatcct tttcattaaa gggtgcaaat
      841 agttcatttg atacaacagc acaggaaatg tctgtttcgg cggacactga tgcagcggcg
      901 gacgaggctc tttcagtgtg ccatgagggt agcctgattg acagatttgt ggagtcagag
      961 gagctcaaaa agggcaatgt tgccgtcaat atgtatctgt cggttgataa acaaaagacc
     1021 gcaaagatga tttatgataa gagcgacgag ctcaatataa aggcagttga caattcgctg
     1081 cagagaaacg gcgacagctt cgattttgtt ccgggacagg aaggcaaaga ggtcgatgtg
     1141 gtaaaatcag tctatgccat aaatgacttt ttgcagaata gctgggatgg ctcggcgaat
     1201 gagatagagc tggtcacaaa tacggttcag ccgagggggt caaaggaaga gctctcaaag
     1261 attaaggata atctgggagg cttttctacc gatttcagca gctcggcagc agggcgtgca
     1321 accaatgtaa agaacgcgtg cagcctgata aacggcagtg tcatatatcc gggtgagcag
     1381 ttctcggttt acgaggctat cagccctatc acaactgaca acggctatca gatagcaggc
     1441 gcatatgaga acggtcaggt agttgactcg gtaggtggag gtgtgtgtca ggttgcgacc
     1501 actctctaca atgcggttat aagggctgag cttgacatag tgcagcgcta caaccattcc
     1561 atgatagtaa gctatgtaaa gccatctgac gatgcggcaa tcgcaggaac ctacaaggat
     1621 cttaagttta aaaataatct ggacaatccg gtgtatatag agggctattg cagtggtgga
     1681 gtcatcacat tcaatgtgta cggagtggag acgagaccgg caaacaggga aatttccttc
     1741 aggagtgaga cgatttccga ggaggatccg gtcacgcagt ttaagtttga tgcaggtcag
     1801 ccggtcggtt atttcaacac cgagcagtct gcgcacaagg gagtgaccgc aaggctatgg
     1861 aagacggtca cagttgatgg aacagtgcag agtgatgagg tattcaacaa cagcaagtac
     1921 aagtcatcac cgaaaatcgt gacggttggt acggcaggag catcagcgga ggttgttgcg
     1981 cagcttcagg cggcagcagc ggctaatgat gagggctcgg ttaaatctat tgctgcgtcc
     2041 gcggcagcag cggctcaggc acaggcagca cagactcaga cacaggatcc gaatgcggca
     2101 gggcagacac agggtgcgca gacccagact acagatccga atgcagcagg gcaggcacag
     2161 ggcacccaga cgcagacgac agaccagaat gcagcagggc aggcacaggg cacccagaca
     2221 cagcagccgg caggacagac gcagtaaatg cccgcaggta gttgtttctg atgtagttat
     2281 ttgcaatcaa tgcaccgggc tgtaacagta tacaaaacat attattatta ggaacaggaa
     2341 aacacatgaa aaacggtaaa atatcagagt cggttttaaa gcgctctgtc ttaaaacaac
     2401 ttcataccac aagcgacaga atacttttta aaccggcagt aggtgaggac tgtgcggtga
     2461 tttccatgca ggcaggcggt caaactaagt tagttactgc tacccagacg ttcacacttg
     2521 atgtatcgga taagcatgtg cgtgctaact gtgcagttta tgatgcattg aacaatattt
     2581 atgccatggg ggcaggccct gttgggatag agctggcact gcttgtacct accacagaaa
     2641 acgaagccgt gcttcgcgag acggtgagag cgattgatgc tgtatgtgag gaagcaggca
     2701 tcgtggtgct tggtgggcat acacaggtga gccgtgcggt gaagaatatc gtggtgacag
     2761 ttacagcaat aggtgaggcc tatgagcagg cgcttacacc atcatcggca gcagcaccgg
     2821 gcatggacgt aatagtgaca ggctatgtgg ggcttgaggg caccgcaatt cttgcaaatg
     2881 agaaaagagc cgagcttgaa acgagatttt ccaaggcatt tatagataaa tcagcagcat
     2941 atatagacca tatttccacc gcaatggagg ctgcatccgc gattggcgcc ggtgtggcgg
     3001 caatccatga cgcttcgcag ggaggcatct ttggagcact ctgggatatg gcagaggctt
     3061 cgggaattgg tcttgacatc gatttaaaga agctccctat caggcaggat acgattgaaa
     3121 taagtgaatt ttttgacata aacccgtata agcttttgtc aggaggaagt ctgataatca
     3181 tagctgcaga tggtacccgc gtggtcagag agcttgagaa aacaggacaa aatgctgtta
     3241 tagtcggtgc cacaaccgat tcaaacgaca gagtgctgat ttccggcgag gagagacgat
     3301 ttttggagac tgcccagact gacgagatat acaaggtttt caactaatat atttgaatca
     3361 ggagattata tatcatgaga gaaaagatat taacatttat agagaaaaac agcaggataa
     3421 acctgaagga gcttgcaatc atgcttggtg tggatgaggc aagtgtggca aacgagattg
     3481 ctgctatgga gcaggagaat gtcatctgcg gatatcacac actgattgac tgggacaagg
     3541 caggccttga gaaggtcaca gccatgatag aggtcagagt gacaccacag cgcggcatgg
     3601 gcttcgacaa gattgccgag cgtatataca actatccaga ggtgaagtct gtatacctta
     3661 tctcaggcgg cttcgacctc atggtcacac tcgaaggtaa gactctgcgc gaggtatcac
     3721 agtttgtgac agataagctt tctacactgg atcaggtgct ttcgacaaag acaaacttta
     3781 ttctgaagaa atacaaggac catggaacta tcatggcggc acctgtcaaa tccgatgaaa
     3841 ggggaattat gtcataatga gagacccttt atccagtacc ataaagacta tccagccatc
     3901 gggcatccgt aagttttttg atatagtgag cgagatgaag gatgcgatat cgctcggagt
     3961 aggtgagcct gattttgaca caccgtggca tatcagggat gagggcatat attcccttga
     4021 aaagggacgc accttctaca cctcaaatgc cggactcaag gagttaaagg ttgagattgc
     4081 aagatatctc gacagaagat acggcatgac atatgattac aacaaagaga tcatggtaac
     4141 tgtcggcggc agcgaggcta tagatatcgc actgcgcgcc atgctcgatc ctggagatga
     4201 agtgattatc ccacagccaa gctatgtttc atatgtgcca tgtactgtgc tggcaggtgg
     4261 cacacctgtg attgtggagc tcaaagagga aaatgatttc aggctgacag ccgaggagct
     4321 tgaggctgtt atcactccaa agacaaagat acttatcatg ccttttccta ataaccctac
     4381 aggcgctatc atggaaaaag cagaccttga agcaatcgtt caggtggtta aggagcatga
     4441 tctgtttgtg ataagtgacg agatttattc agagcttaca tatgtggata agcatgtgtc
     4501 tatagcatcg tttccgggaa tgaaggagcg aacagtgctc ataaacggtt tttccaaggc
     4561 ttatgccatg acaggctgga ggcttggata tgcatgtgca cctgagacca ttttaaagca
     4621 gatgctaaag attcatcagt ttgcaatcat gtgtgcgccg accacgagcc agtatgcggc
     4681 tgtcgaggct atgaaaaacg gcgatgagga tgttgcacag atgagggagg aatacaacgg
     4741 ccgcagacgc tatgtgcttc accgctttaa ggagatgggg cttaagtgct ttgagccttt
     4801 cggggccttt tatgcgttcc caagcatcaa ggagtttggc atgacctccg atgagtttgc
     4861 cacaaagctt ctaaacagca agaagattgc cgtggttccg ggaactgcat tcggagagtg
     4921 cggtgagggc tttttacgta tatcatatgc atattcgctt gatgatttaa aggaggcgct
     4981 cggccgcatt gaggagtttg tcacagagct tcgcgccggg atggataata aatagtttaa
     5041 attgacagga gacaaaatgg caaagctgaa catagtgtta catgagccgg aaattccggc
     5101 aaatacagga aatatcggaa gaacctgcgt cgcgacaggc acgaggcttc atcttataga
     5161 gccgcttgga ttctcacttt cagagaaggc actaaagaga gccggaatgg attactggaa
     5221 ggacctcgac gttaccacgt atgtggactg ggaggatttc cttgagaaaa atccgggcgc
     5281 aaagatatat tatgcgacaa caaagggcag acatgtgtac tctgatgctc attttgagcc
     5341 cgactgctat attatgtttg gcaaggaaag cgcaggcatt ccggaggaga tactaaagga
     5401 gcatccggat gagtgcatca ggataccgat gataggtgag acaaggtcac ttaatctgtc
     5461 aaattcagta gctatcgtgc tctacgaggc attgagacag aataattttg atcatatgag
     5521 gcttgaggga gagcttcatc acctgagctg ggatgactaa atcaagcggc aaagcgcata
     5581 tttttggcag ctatttgggc tttaacagct atgataattg taaatacggt agatttttag
     5641 gaaaaagaaa tgggcataat taaaacaagc aaattagtac atgaatttat acgaagagac
     5701 gaggaaggca atgtagagtc aatcactaca gcactcgaca acgtaaacct cgacataaaa
     5761 gagggacagt ttattgcaat cctcggtcac aatggttcag gaaaatcaac tcttgcaaag
     5821 catatcaatg cacttcttgc gccgagcgac ggcaccatat gggtggatgg catggatgtg
     5881 tcaaaggaag agaatgtcat acctgtcagg aaaagtgcag gtatggtttt ccagaatccc
     5941 gacaatcaga ttatcgccaa tgtcgtggag gaggatgtgg gctttggacc tgagaatatc
     6001 ggtgtgccga cagatgaaat ctggcagcgc gtggactatg cactaagggc tgtcggtatg
     6061 acaaagtacc gcacacattc accgaacaag ctctccggag gacagaagca gcgtgtggca
     6121 atcgcgggag ttgtggcaat ggagccaaaa tgcatagtat ttgacgagcc tacagccatg
     6181 cttgacccaa acggccgcaa ggaggtcata gccacagccc atgagctcaa taagaaaaag
     6241 ggcgtgacca ttattctcat cacccattac atggaggagg tggtcggcgc agaccatgtc
     6301 tatgtcatgg aaaagggcaa ggtggtcatg gacggcacac cgcgtgagat tttttcaagg
     6361 gtggacgagc tgaaggagca caggcttgat gtgccgcagg tgacactggt agccgatgag
     6421 ctcaaaaagg cagggctcga tatccctgat ggaatcctga cgagagaaga acttgtaaat
     6481 gcgcttgttg cgctgaaaaa cagataggcg ggagaaaata tgtcaataat tctagatcac
     6541 ataaactatt gctacagtgc cgactcagcc tacaaggtgc aggcacttaa ggatgtaagc
     6601 cttgagataa atgatggaga gtttattgga ataatcggtc acaccggttc gggaaaatcc
     6661 acacttatcc agcatatgaa cggccttttg aaggcgacaa gcggtcatat atactacaat
     6721 ggacaggata tatatgacaa tgattatgat ttaagcaagc tgcgtcacaa ggtggggatg
     6781 gtgttccagt atccggagca tcagcttttt gagacaacca actttgagga tgtatgcttt
     6841 ggaccgaaaa atcaggggct cgatagaaaa acagtggagc taagagccta tgaggcactg
     6901 cagaatgtac attttccgga ggatctctac tatcagccgc catttgattt gtccggagga
     6961 caaaaaaggc gtgtagccat agcaggagta cttgcaatgc accctgacgt acttatactc
     7021 gatgagccga cagcgggact ggatcctgcg ggcagagatg agatactggg actgctttta
     7081 cagatgaaaa aggatctggg tatcacaatc atactcgtat cacacagcat ggaggatgtt
     7141 gcggagtatg tggatcgcat cattgttatg aattcaggag aggtgatgtt tgacggagtg
     7201 ccgaaggagg tattctcaca ctacaaggag ctcgaggcag tcggacttgc agcaccgcag
     7261 gtcacatatc tgatgcatga tctggcggaa aagggtatac cggttgacct ttcggcaacg
     7321 acagttgcgg aggcgaggga ctcaatacta aaagccttta aatagctcac caatgctgac
     7381 attttgatac aaattatgag gaaggaatac aaaaatgatt cgtgatatta ccataggcca
     7441 gtattatccg gctcagtcgc caatacataa actggaccca agagtaaagc ttttcgcaac
     7501 acttatatat attattgcgc tgttttccta caagggaata gccggtgtgc tgtttatagt
     7561 tgccgcgctt gccgcagtta taaagatatc aaaggtgccc tttaagttca tggtaaaggg
     7621 actcaaatcc attataatac tgctgctttt tacagcatgc ttcagcgtgt ttttcacaca
     7681 gggagagtac acaatctggg agtacggtat actgcacatt tctgacaccg gactgataaa
     7741 tgcagcaacc atgatgatac gacttgtgct gctcattata ggaacctcgc tcatgacact
     7801 caccaccaca ccaaaccagc tcacggacgg tcttgagaaa tcactgggct ttttaaacaa
     7861 aatacatgtt ccggtgcatg cgatagccat gatgatgtcg atagcgcttc gttttattcc
     7921 aatccttatt gaggagacgg acaaaataat gaaagcacag atggcaagag gagctgattt
     7981 tgagcatgga aatatcataa aaagagcaaa gagcatggtg cctcttttag tgccgctttt
     8041 cgtatcggca ttcaggcgtg cggatgacct tgcaatggcc atggaggcca gatgctacaa
     8101 tggtggagag ggcaggacaa agatgaagcc gctcgtatat gaaaaggacg ataaaatagc
     8161 atatcttttc atatttgcgt ttttagcggt tgtgatagtg cttagaatct ttctgccggt
     8221 gaatctgggc aatcttattt tcagatagtg aaatagatag tgcaggcaaa acaggagaga
     8281 catatgcgtg taaaaatgat tgtagcctac gatggcacaa attactgcgg ctggcagata
     8341 cagccaaacg gcataacaat tgagcaggta ttaaacgagc acctgagccg cctgttaaag
     8401 gaggatataa aggtgacagg agcaagccgc accgatgctg gagtgcattc acttggaaat
     8461 gtgtgtatgt ttgacaccaa tacccgcatg ccggcggaaa agatatcgta tgcactgaat
     8521 cagaagctgc cggaggatat agtggtggtc gattcctgtg aggtaagcga tgattttcat
     8581 ccacgcttct caaagagccg taaaacctat gaataccgca tattaaatgc gaggttcaga
     8641 aatcctacac gcaggcttga cacatatttc tatcattatc cgcttgacac agaaaagatg
     8701 agtgaggctg caaaatatct cataggagag catgattttg caagcttttg ctcggcaaac
     8761 tcgcaggcac agaccacagt ccgcacgata tatgactgta cggtgtcaaa ggccggtgac
     8821 atcatcacaa tacgtgtgac aggaaacggc tttttgtaca atatggtgcg cataatcgca
     8881 ggaacacttg tgaagatagg tggaagcgat atgccggcag atgcgatgaa ggacataata
     8941 gccgcaacag acagggcagc agcaggtcct acagccccgg cccatgggct tacaatgata
     9001 ggactcaagt acgagtaaca gctttagatt tttaatatta tattaagatt tttggaatat
     9061 cagtttttag cttcaataat aggctataat gtcgttacag ttaatacacg gtacggtaaa
     9121 aagaagcttg gagatgaaga cggttatgag atttacaaaa gtaaaagaga aaattataaa
     9181 aaacaaaccc ataaacagca cagacgaagg aaaaggcaga catcgtaagg taatccgtat
     9241 aggagcgttt gccctggtat ttgtcgtgct gttttcgttt gtctcgacat tttttaagat
     9301 gacggatgct gtcaatattg cgactataga gggcttttat aaggagccga agaacacaat
     9361 tgatgtgatg atgataggag ccagcgaggt atacgctgat tacagtgcaa ccgaggcctg
     9421 gaagaattat ggatacacat catactcgct tggggtatca ggagtgcccg gcagcctgta
     9481 caaatcaatg ctcagagaag ctctcaccag acagcatccg aaggttgtgg tttttgaagt
     9541 gaatggcttt ttacaaaatg acagctacta cgacagatcg gtaaagctgc atagctggat
     9601 agacaatatc cataatgagg aaaacaggct tgatacaata caagagattg tgccaaagga
     9661 tgagcaggac aactttagaa accctttaaa gctatatcac agcaattgga aggatattga
     9721 caaatgtgcg cacacctttg ccacaagagt tggaatgtat tttgcaaaaa catcatgcat
     9781 gaagggattt tcatctattg caaaatattc cgattgtcca tcaggcaagc agaaaaagct
     9841 ctattttacc gacaagagca gagagtatat gactgatctg cttgagtatt gtaaagcgca
     9901 gggggtggaa aaggtgcttt ttgcgaggtt ccctcatgag aaggagataa agaatccgca
     9961 ggtgctttgt gacgtgaagg agctggtgga gtcttatggc tatgactttg cgagctttga
    10021 aggcgataag gaatttatcg gaatcaatga gagggatgat ttctacaatt cagagcacct
    10081 gaatataaat ggaagcctca aattcactgc atatatggga aaatatctgt ctgataatta
    10141 caggcttaat gcagaacact caccggagct tcagagtgca tggggcgagt gcgcaaggcg
    10201 cgctgacaag gtgataagtg cctgcgcaaa ggatacggat ctgagtctgg gcaatcatta
    10261 ttttgagatg tccgtgtatc tgccttataa tttttcgtca agccttgcga caaataaggt
    10321 agcagatacg aacaacgatg caaagcctgc aaaggagtta aataccccgg taaaaaaata
    10381 gcatgatagt gtatggactt taaaataaat actgcctgtt aattaaagta tgtccggcag
    10441 tattacatta gaaaatagat ttggagtaaa taaaatggaa ctcatttcat taaaatatgc
    10501 gatatttgta atcgtgttgc ttgtactata ttattgcttt ccgaagaaat accgctggta
    10561 tgtgcttctg gcgggaagca tggcatacta tgtgattata tgtaagtggt atgtgctttt
    10621 tatcattttc acaatatgca ccacctatgg ctcaacaata tggattgaca agctgttaaa
    10681 gaagcagaat gctatcgtaa aaagccataa ggaggactgg gacaggcaga ccagaaagga
    10741 ctacaaggag aagggcagaa aaaagagagt ggcagtcatg ctttttgcgc tgctttgcaa
    10801 tttcggcatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttctacac cttccagtcc atgggttatg
    10921 tgatggatgt atacagggaa attgtcgagc ctgaaaagaa ttttcttaag gtagcgctgt
    10981 tcgtgtcatt tttcccacag atcatacagg gaccgattgc gatatatgat aagcttgcgg
    11041 gacagcttta cgagggacat tccctgagac ttgaaaatct gcaaaagggt gcgcttctgg
    11101 tgctctgggg agtcatgaaa aagcttgtga tagccgaccg cgcggtaaat atcatcaatt
    11161 ttgtgatgga taagccgatg gatttttcgg gcacttatgt attttttgcg gcagtagtgt
    11221 atgcgctgca gctatatgcg gatttctcgg gaggaatcga tatagtgcgc ggtattgcgg
    11281 agatgtttgg cataacaatg tcaacaaact tcaaccatcc gtatttttcg cgctcactca
    11341 cagagtactg gcaccgctgg catatgactc ttggagactg gtgcagaaac tatatcttct
    11401 acccgctttc aatctcaaag cgtttcctga actttggcaa atggctaaag ccgagatttg
    11461 gtgcgcatat cagtaaggtg ctgccgggct gcatcgcgag tgtcatcaca tttatcgtca
    11521 tcggagtatg gca
//
LOCUS       DPUF01000025            4346 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_31161, whole
            genome shotgun sequence.
ACCESSION   DPUF01000025 DPUF01000000
VERSION     DPUF01000025.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_31161"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>712)
                     /locus_tag="DHV81_00860"
     CDS             complement(<1..>712)
                     /locus_tag="DHV81_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI93097.1"
                     /translation="VMILSLLLTGCSGRKDDGTVTITNVSYDPTREFYEKYNKIFESY
                     YESEHGKKINVIQSHGGSGAQARSVVEGCNADVVTLALEHDIDLIQNTGLIEKGWIDE
                     FPKSSAPYTSTIVFLVRKGNPKHISDWDDLVKKNVDVITPDPKSSGGACWNFLAALSY
                     AKTNYDSQKEQKTFMRKLYENVTIMDSGARGSTTTFVENGQGDVLIAWENEALTTMDS
                     YPGKYEIVNPSVSILAQPS"
     assembly_gap    713..771
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(798..1733)
                     /gene="cysK"
                     /locus_tag="DHV81_00865"
     CDS             complement(798..1733)
                     /gene="cysK"
                     /locus_tag="DHV81_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCI93098.1"
                     /translation="MAKIARQLTELIGHTPLLELTNYEKALGLEAKIIAKLEYFNPLG
                     SVKDRVAAAMIEQGIKDGKINQDTIIIEPTSGNTGIGLAFVAASKGLHLILIMPDTMS
                     VERRKIVTALGAEIKLTPGREGMKGAIDEAQRLKEHYGNAFIPQQFENQANPDIHRAT
                     TAQEIWEDTDGKVDIFVSSVGTGGTCTGTGLGLRDHNPDVRIVAVEPKSSPVLSGGRP
                     GPHKIQGIGAGFIPKVMRMDIVDEVIPVENEAAFDKAREVAKRDGLLVGISSGAAIYA
                     ATELAKRPENKGKNIVVLLPDTGERYLSTPLFTVN"
     gene            complement(2011..3576)
                     /locus_tag="DHV81_00870"
     CDS             complement(2011..3576)
                     /locus_tag="DHV81_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCI93099.1"
                     /translation="MILAAEYVPSMYATVWALVPPVVAIVLALITKEVYSSLFVGIVI
                     GGLFYGNAFQPGFSAERSVLHIFEDGLVGVLSDSYNVGILIFLVVLGVMVSMMNKAGG
                     SAAFGEWASEHIKTRIGAQLSAIMLGVLIFIDDYFNCLTVGSVMRPVTDKHQVSRAKL
                     AYLIDATAAPVCIIAPISSWAAAVTGFVKGEDGFSIFIKAIPYNYYALFTIIAMVTLV
                     VLQVDFGPMAKHEANAKKGDLFTTGDRPYAEVKQDVIKGKGKVIDLVFPILVLIISCI
                     IGMIYTGGFFDGTGFVDAFAGSDASIGLMLGSFFALIITICFYSIRRVLSFTDCCNSI
                     PEGFKAMVPAILILTFAWTLKTMTESLGAKEFVAGLVGGVSGPLLGLFPAIIFLVGAF
                     LAFATGTSWGTFGILIPIVVNIFSGTNHTMMIISISACMAGAVCGDHCSPISDTTIMA
                     SAGAQCNHMNHVSTQLPYAVLVAGISCLTYIIAGFVRNPVVPFIIGMLILIGTFVGIK
                     YITRTDKANEQEE"
     gene            complement(3606..>4346)
                     /locus_tag="DHV81_00875"
     CDS             complement(3606..>4346)
                     /locus_tag="DHV81_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCI93100.1"
                     /translation="TPRVAEVYGGMLNAVGLQNPGIELFCKRDIPFLKQFDTRIVVNV
                     CGHSLDEYLDVVKRLADEPIDMMEINISCPNVKEGGIAFGTDPKGVETITSEIKKYAK
                     QPVIMKLSPNVTSIAEIARAAEAGGADAVSLINTITGMKIDINRRSFVLANKTGGMSG
                     PAVHPVAVRMVYETAHAVNIPIIGMGGITNASDAIEMILAGATAVSVGTANFVNPKTT
                     EQIVDGIAEYMDRYGVKEISELVGAVDR"
BASE COUNT         1070 a         1089 c          852 g         1276 t
ORIGIN      
        1 gagggctgtg ctaagatgct gacagacgga ttcactatct catacttgcc cggatatgaa
       61 tccatggtcg taagtgcctc gttttcccac gctataagca catctccctg accgttttcc
      121 acaaatgtgg ttgttgagcc tctcgcacct gagtccataa ttgtgacatt ttcatacagc
      181 tttctcataa acgttttctg ttccttttgg ctgtcatagt ttgtcttcgc atagcttagc
      241 gccgcaagga aattccagca ggctccgcca ctgctctttg gatccggtgt aatcacatcc
      301 acattctttt tcaccaggtc atcccagtcg cttatatgct ttggattgcc ctttcttaca
      361 agaaaaacga tggttgatgt gtacggtgca gagctttttg gaaattcatc aatccagccc
      421 ttttcaatta agccggtatt ttgtatcagg tctatgtcat gctcgagtgc cagtgtgaca
      481 acatctgcat tacagccctc cacaaccgaa cgcgcctgcg ctcccgagcc gccatgtgac
      541 tgaatcacat ttatcttttt gccgtgctcg ctctcatagt aagactcgaa tattttgtta
      601 tatttctcgt aaaactctcg tgtcggatca tacgacacat tggttatggt gactgtaccg
      661 tcatccttcc tgcctgagca gcctgtcaga agaagtgaca gaatcatcac tgnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcttatgc
      781 cgtcagttta tatgccatta gtttactgta aaaagcggtg tggacaggta tctttctcct
      841 gtatccggaa gcagcaccac gatattcttt cccttgttct caggtctttt cgcaagctct
      901 gttgccgcat atattgctgc tcctgaggat atgccaacga gaagtccgtc tctctttgca
      961 acctctcttg ccttgtcaaa agcggcctca ttctccaccg gaatcacctc atccactata
     1021 tccatgcgca tgacctttgg aataaagcct gcaccgattc cctgtatctt gtgaggtccc
     1081 ggtcttccgc ctgagagtac cggtgagctc tttggctcaa cagctactat ccttacatca
     1141 ggattatggt ctctcaatcc aagcccggta cctgtacagg tgccgcctgt tcccacagat
     1201 gatacaaaga tatctacctt tccatctgta tcctcccaga tttcctgagc ggttgtagct
     1261 ctgtggatat ccggattggc ctgattctca aactgctgtg gaataaaggc attgccataa
     1321 tgctctttaa gtctctgcgc ctcatcaatt gcacccttca tgccctcacg ccccggtgtg
     1381 agctttatct ctgcgccaag cgctgtgact atttttcttc tctcaacgct catggtatca
     1441 ggcattataa gtatcaggtg aagccccttt gaggctgcca caaatgcaag tccgattccg
     1501 gtattgccac ttgtcggctc tatgattatt gtgtcctgat ttatctttcc atcctttatg
     1561 ccctgctcta tcatagctgc cgccaccctg tcctttacac ttccaagtgg attgaaatac
     1621 tccagctttg ctatgatttt tgcttcaagc ccaagggctt tttcataatt ggtcagctcc
     1681 agaagcggag tgtgacctat cagctcagtg agttgccttg caatttttgc cattgatatt
     1741 acctcttttt tgttaatgtt tgttttgagt ttgttatgag tttgagctta gtttgtatgg
     1801 ctgggatttc ctaccaatca aatatgattt ctaagttatt gatatgttac cactaattga
     1861 tatttttgta aatgtaatta gggttattaa tatttgttag cgggctataa attttattga
     1921 tagcagatag aatttactat aaaaaaagag ttctatcatt cgcagacaca aggtctgtga
     1981 aaaatagaac tcttttgagt tgcgctttgg ctactcctcc tgttcatttg ctttatcggt
     2041 tctcgtgata tacttgattc cgacaaaggt tccaatcagg ataagcattc ctattataaa
     2101 cggtactacc ggatttctga caaagcctgc tatgatgtag gtcaggcatg atatgcctgc
     2161 cacaagcact gcgtacggaa gctgtgtgga tacatgattc atgtgattgc actgagctcc
     2221 tgccgatgcc atgatggtgg tatcggatat cggtgagcag tggtctccac atactgcacc
     2281 tgccatacac gctgagattg agataatcat catcgtatgg tttgtgcccg agaagatatt
     2341 tactactatc ggtatgagta taccgaaggt gccccatgag gttcctgttg caaaggcaag
     2401 gaaggctccc accaggaata tgattgccgg gaacagtcca agcagaggtc ccgatactcc
     2461 tccaactaag cctgcgacaa attccttggc gccaaggctc tctgtcatgg tctttagtgt
     2521 ccatgcaaag gtcagtataa gtattgcagg taccattgct ttaaagccct cagggattga
     2581 attacagcaa tccgtaaagc ttagtaccct tctgattgag taaaagcata tggtaattat
     2641 caacgcaaaa aagcttccaa gcataagacc aattgaagca tcgctgcctg caaacgcatc
     2701 aacgaagcct gttccgtcaa aaaagcctcc tgtgtatatc attccgatga tgcagcttat
     2761 tatgagcaca agaataggaa atacaaggtc tataaccttc cccttgccct ttataacatc
     2821 ctgctttacc tctgcataag gcctgtctcc tgtggtaaac aaatcaccct ttttagcatt
     2881 ggcttcatgc tttgccatcg gaccgaaatc cacctgcaaa acaacaagtg tcaccatagc
     2941 gataatggta aagagtgcat agtaattgta tggaatggcc tttatgaaga tagaaaagcc
     3001 atcctctccc tttacaaaac ctgtgaccgc ggctgcccat gatgatatag gtgcgataat
     3061 gcatacaggc gctgctgtgg catctatcag atatgcaagc tttgctcttg aaacctgatg
     3121 cttatcggtg acagggcgca tcacacttcc tactgtcaga cagttgaagt aatcatctat
     3181 aaatatgagc actccaagca ttatagccga aagctgggca ccaatccttg tctttatgtg
     3241 ctcacttgcc cattctccaa aggccgccga gcctcctgcc ttgttcatca tgctcaccat
     3301 gacaccgagc acgaccaaaa atatcagtat tcccacattg tacgaatccg atagtactcc
     3361 aacaagtccg tcctcgaata tgtgaagcac tgatctctca gccgaaaaac ccggctgaaa
     3421 tgcatttcca taaaacagtc cgccaatcac tattccaacg aataatgagc tgtagacctc
     3481 ttttgtgata agtgccagaa ctatagccac aacaggtgga acaagtgccc agactgtggc
     3541 atacatagaa ggcacatatt ctgctgccag aatcattatt ttatcctccg tctatccatt
     3601 atgctctatc tgtcaaccgc gccgacaagc tctgaaatct cctttactcc atacctgtcc
     3661 atatactctg cgatgccatc cactatctgc tctgtggtct ttggattcac aaagtttgct
     3721 gtgccgactg aaacagccgt agctccggca agtatcatct cgatggcatc agaagcattt
     3781 gtgataccgc ccattccgat aatcggaata ttgactgcat gtgctgtctc gtaaaccatg
     3841 cgcactgcaa ccggatgcac agccggaccg ctcatgccgc ctgtcttgtt tgctaacaca
     3901 aagcttcttc tgtttatatc aatcttcatg cctgtaattg tattgatgag cgaaaccgca
     3961 tcagctccac cggcctctgc cgcccttgct atctctgcaa tacttgtgac atttggagaa
     4021 agcttcatga taacaggctg ctttgcatat tttttaatct cgcttgtgat tgtctcaaca
     4081 ccctttggat ctgttccgaa agcaattccg ccctccttaa cattagggca tgagatattt
     4141 atttccatca tatcaatagg ctcatccgca agacgcttta ccacatcaag atactcgtca
     4201 agcgaatgac cgcagacatt taccacaatc cttgtatcaa actgctttaa aaaaggaata
     4261 tccctcttgc agaaaagctc gatgccggga ttttgaagtc cgactgcatt gagcatgccg
     4321 ccgtacacct ctgctactct cggtgt
//
LOCUS       DPUF01000026            8458 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_22133, whole
            genome shotgun sequence.
ACCESSION   DPUF01000026 DPUF01000000
VERSION     DPUF01000026.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_22133"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            98..859
                     /locus_tag="DHV81_00880"
     CDS             98..859
                     /locus_tag="DHV81_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCI93101.1"
                     /translation="MSAEKIGDIKEQLKSITDSQLTQFIEAYGSDERGGVIKLVDSAK
                     KRLDKYEKELIRTDGLKKYEREYASYAHICGIDEVGRGPLAGPVVACAVILPKDCDIL
                     YINDSKKLTAAKRDELYDVIMEKAVSVGIGMASHERIDEINILQATYEAMRQAIKKLD
                     PAPDLTLNDAVTIPGVDIKQVPIIKGDAKSISIGAASIVAKVTRDRMMVEYDRIYPGY
                     GFASNKGYGSAEHIKALKEIGPSPIHRASFIHNFI"
     gene            952..1857
                     /locus_tag="DHV81_00885"
     CDS             952..1857
                     /locus_tag="DHV81_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI93102.1"
                     /translation="MTLQQLRQVLTIAESNSMNEAAKKLFVSQPNLSATVREVEEELG
                     ITVFMRNNRGITVTAEGEEFLGYAKQVVEQYHLLENRYLNVESKKKFSVSMQHYSFAV
                     KAFVQLAKEVGMDEYEFAVHETKTKEVIDNVKNLKSEIGVLYLSDFNEKVLRKLFKEC
                     DIEFKELFTCNTYVYLWKKHPLAGKKVITLDDLQEYPCLTFEQGVNNSFYYAEEMMST
                     YEYKRVIKADDRATMLNLMVGLNGFTLCSGIISEDLNGDDYAAIPLKESEKMHIGYIK
                     HKGTKLSKLGEIYIDALKNYENKVL"
     gene            2139..2372
                     /locus_tag="DHV81_00890"
     CDS             2139..2372
                     /locus_tag="DHV81_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCI93103.1"
                     /translation="MYNKETVIRLNTLDDVQEFVGAASKCDFDIDLKYRQILIDAKSL
                     LGVIGIGINNDFKVCYMGDDQAFDNVVDKFTVA"
     gene            2428..>3206
                     /locus_tag="DHV81_00895"
     CDS             2428..>3206
                     /locus_tag="DHV81_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCI93104.1"
                     /translation="MFSVLKISGVRLKILPEHKTVDIIRNMQGEYMTEAGNNYSEKKT
                     QLHISVRNLVEFIFREGDIDNRSSRAMSADAMMEGTRIHRKIQGSMGKEYQAEVPLSL
                     VVEGDLYELTVEGRADGIFTEDGKCFVDEIKGMYRRVELFEKPVFVHRAQAMCYAYIF
                     ALQNNMETIGIQMTYCNLETEQTKYFREEFSFEEIKKWFDDLMEEYGKWATFQCEMKN
                     QRQASIKELDFPYEYRPGQKKLVSDVYRTIMRQKLLFMQAPT"
     assembly_gap    3207..3311
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3312..4907
                     /locus_tag="DHV81_00900"
     CDS             <3312..4907
                     /locus_tag="DHV81_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCI93105.1"
                     /translation="ITATVAKETFALLEKNGYRAKTIQITAKEKLCPCDEMECNPVTC
                     PYAKGHFDRVNDAVFDLLHRCEMIERDDILSQADRYTVCPFELCLDTASWCDNVICDY
                     NYVFDPNVYLKRFFQEGIKGDYIFLIDEAHNMVERSRQMYSAQIYKEDFLTVKRIMKE
                     HSRSIEKALEKCNKILLGMKRECENYTVYDTFGNMVFSFMRLMTLLDEFLQKANEFPG
                     KRDVMDFYFELRNFLNIYDLVDEHYVMYSELEADGRFMLKLFCVDPSLNIQKRLDKGK
                     SAVFFSATFLPVNYYKSLLSTKKDNYAIYADSTFDSKKRLLAMATDVSTRYTRRSRSE
                     YERIAGYINAVVTQKTGNYMVFFPSYKMMNDVADIYCEKYADETELMLQKNNMSEAER
                     EEFLDRFSEESDRTLVAFGIMGGIFGEGIDLKNDRLIGAIVVGTGLPQISNERTILKD
                     YYDAENGCGFDYAFRYPGINKVLQAAGRVIRTTEDTGVILLLDERFWQREYDLLYPRE
                     WSDRKPCNIANVGKLVADFWEQI"
     gene            complement(4878..5768)
                     /locus_tag="DHV81_00905"
     CDS             complement(4878..5768)
                     /locus_tag="DHV81_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI93106.1"
                     /translation="MDINYELYKVFYYVATSLSFSEASKKLFISQSAVSQSIKTLEKK
                     LGQTLFIRSTKKVQLTAAGALLLKHIEPAMNLIARGESQLLDSGTLGLGQLHIGASDT
                     ICRYLLVPYLKQFHQRFPNVPIKVTNATSIQCVELLEQRKVDLIVTNFPNSHLNHSYR
                     QKTICNFSDVFVANPNYFKLRKQEIAFSELNKYPIMMLDRNSTTSEFLHNLFLQHQLD
                     LIPEIELSSNDLLIDLAKIGLGIAFVPDYCINHENKELFVLKTKEKMPPRQIVVSVNT
                     TMPVSASTEEFLNLLPEVGN"
     gene            6021..>8458
                     /locus_tag="DHV81_00910"
     CDS             6021..>8458
                     /locus_tag="DHV81_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCI93107.1"
                     /translation="MSNVRRVYVEKKPDFAVKAKELKHEIKHYLGITTVEAVRVLIRY
                     DVENISEETYKKALYTVFSEPPVDDIYEETFDYGDAKVFTVEFLPGQFDQRADSAEQC
                     VKLLNENEEPIIRSAITYVIEGTITDEQFAAIKKHCINPVDSRAIGMEKPKTLVQEFD
                     DPADVIIFDGFKDMAEDKLKELYSSLNLAMTFKDFLHIQNYFKNEEKRDPSMTEIRVL
                     DTYWSDHCRHTTFSTELKNVKFDDGFYRTPIENTYKDYLNTFSNIYKDRNDKFVCLMD
                     LALLAMKRLKAEGKLDDQEESDEINACSIVVPVEVDGKTEEWLVNFKNETHNHPTEIE
                     PFGGAATCLGGAIRDPLSGRTYVYQAMRVTGAADPTVPVSQTIEGKLPQKKLVREAAH
                     GYSSYGNQIGLATGYVKEVYHPNYVAKRMEIGAVMGAAPRRAVQRLNSDPGDKIILLG
                     GRTGRDGIGGATGSSKAHNTKSTSVCGAEVQKGNAPTERKLQRLFRREEVSHIIKKCN
                     DFGAGGVSVAIGELADGLRIELDKVPKKYAGLDGTEIAISESQERMAVVVAPSDVEQF
                     LAYAKEENLEATEVAVVTEDPRLVLEWRGKEVVNISRAFLDTNGAHQETDVEVEMPCE
                     SDNYLDKISTPAVARMVEAGDMKGAWEAELEDLNVCSQKGLVEMFDGSIGAGSVYMPY
                     GGRYQLTETQSMVAKLPVLKGKCDTVTMMAYGFDPYLSSWSPYHGSIYAVLESISRIV
                     AAGGDYSKIRMTYQEYFRRMNEDPKRWSQPFAALLGAYDAQIGFGLPSIGGKDSMSGT
                     FNDIDVPPTLVSFAV"
BASE COUNT         2580 a         1442 c         2088 g         2237 t
ORIGIN      
        1 atagatgatt tcagaagcgg taaattaggc catatcactt tggagagacc ggagatatag
       61 aagaatagtt acgaagaata gacgatagga caaggatatg tcagcagaaa aaataggcga
      121 tattaaggaa caacttaaaa gcatcacaga ttcacagctt acacagttca ttgaggcata
      181 cggctcagat gaaaggggcg gagtgattaa gcttgtagat agtgcaaaaa agcgtctgga
      241 caagtatgag aaggagctta tccgcacaga cggtctcaag aaatatgagc gtgagtacgc
      301 atcatatgct catatttgcg gcatagatga ggtgggacgc ggaccgcttg caggtccggt
      361 tgtggcatgt gcagttattc tgccaaagga ctgtgatatt ttatatatca atgattcaaa
      421 gaagcttacg gctgcaaagc gtgatgagct ttacgatgtc ataatggaaa aggcggtttc
      481 tgtaggaata ggtatggcat cgcacgagcg gatagatgag ataaatatac tgcaggctac
      541 atatgaggcg atgagacagg cgattaaaaa gcttgatccg gcaccggatt tgacacttaa
      601 cgatgcagtc acaatcccgg gggtggatat aaagcaggtt ccgattataa agggagatgc
      661 caaaagcata tcaatagggg cagccagtat tgtggctaag gtcacaaggg atcggatgat
      721 ggttgagtat gatagaatat atcccggcta tggatttgca tcaaacaagg gctatggctc
      781 ggcagagcat atcaaggcac taaaggagat aggaccatca ccgatacaca gggcatcgtt
      841 tatacacaat tttatttaat atattacaat tgtgcggttt ttacgaagat gcctgggcgc
      901 tgaatagtta cacattctgc taaaaaccgt cagttcacaa taggagagaa aatgacactt
      961 caacaactca gacaggtact tacaattgca gaatccaatt ccatgaatga ggcggcaaag
     1021 aagctttttg tttcacagcc aaatctgtcc gccacagtac gagaggtgga ggaggagctt
     1081 ggaatcacgg tattcatgag aaataaccgt ggaattacag tcacagcgga gggcgaggag
     1141 tttttaggat acgccaaaca ggttgtggag cagtatcatc tgcttgagaa ccggtatctg
     1201 aatgtggagt ccaaaaagaa attctcggtt tccatgcagc actattcatt tgcagtaaag
     1261 gcctttgtac agcttgctaa agaggtgggc atggatgagt acgagtttgc tgtccacgag
     1321 acaaagacga aagaggtaat agacaacgta aagaacttaa agagtgagat tggagttctt
     1381 tatttaagtg atttcaatga aaaggtttta agaaagcttt ttaaggaatg tgacattgag
     1441 tttaaagagc tcttcacatg caatacatat gtgtatctct ggaaaaaaca tccgcttgcc
     1501 ggcaaaaaag tgattacact tgatgatttg caggaatatc catgtctgac ctttgaacag
     1561 ggcgtgaaca attcctttta ttatgctgag gagatgatga gcacgtatga gtacaagcgt
     1621 gtcataaagg ctgatgacag agccacaatg ctcaatctca tggtggggct caacggattt
     1681 acactgtgct caggtattat ttctgaggat ttaaacggtg atgactatgc ggcaattcca
     1741 cttaaggaaa gtgaaaaaat gcatataggc tatatcaagc acaagggcac gaagcttagc
     1801 aagctcggtg aaatatatat agatgcgtta aagaattacg agaataaagt tttataaatg
     1861 aaaaattgta aaaaatgaac ttgaattaat taaatatcat gccatttttg taagaatttc
     1921 acagttatat tgttttttnn nnnnaaaaaa catgcatgaa aatatagtga aaaatcggtt
     1981 acgcaaaagt tatttattaa aaaaaatgac atgaattgac ggaaaatgac ggattatgat
     2041 tttttttgac aaatgaatat cagatgttat aataggcgca acaattgatt ggcatgtgcg
     2101 aaagcactat gttttagatt ataaaggtgg tgttaactat gtacaataag gaaacagtaa
     2161 tcagacttaa cacattggat gatgttcagg aatttgttgg ggctgcttct aagtgcgatt
     2221 ttgacattga tctcaaatat agacagattc ttattgatgc gaaatcactt cttggagtga
     2281 ttggaattgg tataaacaat gactttaagg tctgctatat gggcgatgac caggcatttg
     2341 ataatgtggt tgataaattc actgtagcgt aatacaattg aataagatcc tttgggagag
     2401 tttcttcttg gagctctcct tctttttatg ttcagtgtat taaaaatatc tggagtcaga
     2461 ctgaaaatat tgccggaaca caaaaccgtt gacataatcc ggaatatgca gggagagtac
     2521 atgacagaag ctggaaataa ctatagtgaa aagaaaacac aattacatat ttcggtcaga
     2581 aaccttgtgg aatttatttt cagggagggt gacatagaca accggtcatc cagagccatg
     2641 agtgccgatg ccatgatgga gggaacacgc attcacagga aaatccaggg cagcatgggg
     2701 aaggagtatc aggcagaggt gccgctttca cttgttgttg aaggtgattt gtatgagctg
     2761 actgtcgagg gcagggcaga tggcatcttt acggaggatg gaaagtgttt tgttgatgag
     2821 attaagggaa tgtacagaag ggttgagctg tttgaaaagc cggtttttgt gcaccgtgcg
     2881 caggctatgt gctatgcata tatttttgct ctgcaaaata atatggaaac gataggcatt
     2941 cagatgacct actgcaatct ggaaacggag cagactaagt attttcgcga ggagttctca
     3001 tttgaagaga taaaaaaatg gtttgatgac ctgatggaag agtatggcaa atgggcaacg
     3061 tttcagtgtg aaatgaaaaa tcaaagacag gcttcaatca aagaactcga ttttccatat
     3121 gagtacagac cggggcagaa aaagcttgtt tcggatgtct acaggacaat tatgcgtcag
     3181 aagcttcttt ttatgcaggc tccgacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn natcaccgca acagtcgcaa aggaaacgtt tgcgctgctt gaaaaaaatg
     3361 gttacagggc aaaaactatt cagataacgg caaaggaaaa gctatgtccg tgtgatgaga
     3421 tggaatgcaa tccggtaacc tgtccgtatg caaaggggca ttttgacaga gtaaatgatg
     3481 cggtttttga cctgcttcac agatgtgaaa tgattgagag ggatgatatt ttatcacagg
     3541 cggataggta taccgtttgt ccgtttgagc tttgtcttga tacggctagc tggtgtgata
     3601 atgtcatctg cgattacaac tatgtgtttg acccgaatgt ttatcttaag agattttttc
     3661 aggaggggat aaagggagat tatatttttc tcattgatga ggctcacaat atggtcgaaa
     3721 gaagcaggca gatgtatagc gctcagattt ataaggagga tttccttact gtgaagcgca
     3781 ttatgaagga gcacagccgt tccattgaga aagcgcttga aaagtgcaat aagatactgc
     3841 ttgggatgaa gcgtgagtgt gagaattaca cggtatatga tacgtttggt aatatggtat
     3901 tttcctttat gaggcttatg acacttctgg atgagttttt gcagaaggct aatgagtttc
     3961 cggggaaaag ggatgttatg gatttttatt ttgagcttcg caatttcctg aacatttacg
     4021 atctggtgga tgagcactac gtgatgtatt ccgagcttga ggctgatggc aggttcatgc
     4081 taaagctttt ttgtgtggac ccatcgctta atatacaaaa gaggctggat aagggcaagt
     4141 cggctgtgtt tttttcggcg acatttttgc ctgtaaacta ttacaagagc ctgttaagca
     4201 caaaaaaaga caactatgcc atatatgctg acagtacgtt tgacagcaaa aagaggctgc
     4261 ttgcgatggc aaccgatgtg agcacgaggt acacaaggcg ctcaaggagt gagtatgagc
     4321 ggattgcagg atatattaat gctgttgtca cgcaaaagac tggaaactat atggttttct
     4381 ttccgtcata taagatgatg aatgatgtgg cagatattta ctgtgaaaaa tatgcagacg
     4441 aaacagagct tatgctgcag aaaaataata tgtcagaggc tgagagggag gaatttctgg
     4501 acaggtttag tgaggaatcg gacagaacac ttgtggcatt tggtatcatg ggaggcatat
     4561 tcggtgaggg cattgatttg aaaaacgaca gactgatagg tgctattgtt gtcggaacgg
     4621 gacttccgca gatcagcaat gagagaacta tattaaagga ttattacgat gccgaaaacg
     4681 gctgtggctt tgactatgct ttcaggtatc cgggcataaa taaagtcctc caggcggccg
     4741 gaagagtaat caggacgacg gaggacacag gtgtcatttt gctattagat gagagattct
     4801 ggcagagaga atatgatttg ctctatccac gtgagtggag tgacagaaag ccatgcaata
     4861 tagcaaatgt cggaaaacta gttgccgact tctgggagca gatttaaaaa ttcctcagtg
     4921 gatgctgaca cgggcattgt agtgtttacg gacacaacaa tctggcgtgg aggcatcttt
     4981 tcttttgtct tcagcacgaa aagctcctta ttctcatgat tgatgcagta gtcaggcacg
     5041 aatgcgatac ccagtccgat tttggccagg tctatgagaa gatcgttact gcttagctca
     5101 atttccggaa tcaggtcaag ctggtgctgt aaaaacagat tatgcaggaa ttcactggtg
     5161 gtgctgtttc ggtcgagcat catgatagga tatttattga gctcggaaaa agctatctcc
     5221 tgcttgcgaa gcttgaaata attgggattt gccacaaaaa catcggaaaa gttgcatatg
     5281 gttttctgtc tgtatgagtg attgaggtgc gagtttggaa aattcgtgac aatcaggtca
     5341 acctttcttt gctccaaaag ctccacacac tggatagatg tggcatttgt gaccttgata
     5401 ggcacattcg gaaacctctg atggaactgc tttaagtaag gaaccagcag ataacggcat
     5461 atggtatcgc ttgcgccgat gtgaagctgg ccaagtccaa gtgtgcctga gtctaaaagt
     5521 tggctttcac cgcgggcaat aagattcatg gcaggctcaa tgtgttttaa aagcagggca
     5581 ccggctgcgg tgagctgtac cttctttgtg cttctgatga aaagagtctg tcccagcttc
     5641 ttttcgagag tcttaataga ctggcttacc gcggactgtg aaataaacag cttcttgctg
     5701 gcttcagaga agcttagtga cgttgcaacg tagtaaaaga ccttgtaaag ctcataattt
     5761 atatccattt tgttatcctc cgtataaata tggttatttg catctgtttt tatcaatgag
     5821 gggcaaaata ccttttgacc cttatatcat tatataagaa aactataatt aacgcaactt
     5881 ataaaacaaa ttaaatatat taattatact tatcataact aaagtgttat actacaacca
     5941 tagttttaaa gtccgattgt gcaaaaagtc ggacggtaac gaataattgg ttacatactc
     6001 tcgaattgaa aggagaacat atgagtaacg tacgtcgtgt atatgtagag aaaaaacctg
     6061 actttgcggt aaaggcaaag gaattaaagc atgagattaa acattatctg ggaatcacaa
     6121 ctgtggaggc agtacgtgta ttaatcagat atgatgtgga aaatatatca gaggagacat
     6181 acaagaaagc cttgtataca gttttctcag agccacctgt tgatgatatc tatgaggaga
     6241 catttgacta tggtgatgca aaagtattta ctgtagagtt tttaccggga cagtttgacc
     6301 agagagcaga ttcagccgag cagtgtgtga agcttctcaa tgagaatgag gagccgatta
     6361 tcagaagtgc tatcacatat gtgattgagg gtactatcac agatgagcag tttgctgcta
     6421 ttaagaagca ttgcatcaac cctgttgatt cgcgtgctat cggcatggaa aagcctaaga
     6481 cacttgtgca ggagtttgat gatcctgctg atgtcatcat atttgacggc ttcaaggata
     6541 tggcagagga caagctgaag gagctttatt catcattgaa ccttgccatg acctttaagg
     6601 attttcttca tatccagaat tattttaaga atgaggaaaa gcgtgatcct tcaatgacag
     6661 aaatccgtgt gctcgataca tactggtcag atcactgccg tcatacaaca ttctctacag
     6721 agcttaagaa tgtcaagttt gacgatggct tctaccgcac accgattgaa aatacctaca
     6781 aggattatct caatacattt tcaaatatct acaaggacag aaatgacaaa tttgtctgcc
     6841 tgatggatct tgcacttctt gccatgaaga gattaaaggc agagggcaag cttgatgatc
     6901 aggaagagtc tgatgagatc aacgcatgct ctatcgtggt acctgttgag gtggatggaa
     6961 agacagaaga gtggcttgta aacttcaaga acgagacaca caaccatcct acagagattg
     7021 agccttttgg tggcgctgct acatgccttg gcggagcaat cagagatcct ctctcaggac
     7081 gtacatatgt atatcaggca atgcgtgtaa caggtgcagc agatcctaca gtaccggttt
     7141 cacagacaat tgaaggaaaa cttccacaga agaagcttgt cagagaggca gctcacggat
     7201 acagctcata tggaaaccag atcggacttg caacaggcta tgtcaaggag gtttaccatc
     7261 caaattatgt tgcaaagcgt atggagatcg gtgcagttat gggtgcggct ccaagacgtg
     7321 ctgtccagag acttaactca gatccgggag ataaaatcat tctcctcgga ggacgcacag
     7381 gacgtgacgg aatcggtggt gctacaggtt catcaaaggc acacaataca aagtctacat
     7441 cagtatgcgg agctgaggta caaaagggta atgcacctac agagagaaag cttcagagac
     7501 ttttcagaag agaagaggtc agccatatca tcaagaaatg taacgatttc ggtgccggcg
     7561 gagtttctgt tgctatcgga gagcttgctg acggacttcg catagagctt gacaaggtac
     7621 caaagaaata cgcaggtctt gacggaacag agattgctat ctcagagtca caggagcgta
     7681 tggcagttgt agttgcgcct tctgatgttg aacagtttct tgcatacgca aaggaggaaa
     7741 atctcgaggc aacagaggtt gcggtagtta cagaggatcc aagacttgtg cttgagtgga
     7801 gaggcaaaga ggtagtaaat atctctcgtg cattccttga tacaaacgga gctcatcagg
     7861 agacagatgt tgaggttgag atgccttgcg agagcgacaa ttatctggac aaaatcagca
     7921 cacctgctgt agccaggatg gttgaggcag gagatatgaa gggtgcctgg gaggcagagc
     7981 ttgaagacct taacgtatgt tcacagaagg gacttgttga gatgttcgac ggctctatcg
     8041 gagcaggcag tgtatatatg ccatatggtg gacgttacca gctcacagag acacagtcaa
     8101 tggttgcaaa gcttcctgta ttaaagggta aatgtgatac agtaacaatg atggcttacg
     8161 gcttcgaccc atatctttca tcatggagcc cataccacgg atctatatat gcagtgcttg
     8221 agtctatctc aagaatcgtt gcagcaggcg gagattacag caaaatccgt atgacatatc
     8281 aggagtactt ccgcagaatg aatgaggatc caaagcgctg gagccagccg tttgcagcac
     8341 tcctcggagc atatgatgct cagattggct tcggacttcc ttcaatcggt ggaaaggatt
     8401 ccatgtcagg taccttcaat gatattgatg taccgcctac actcgtatca tttgcagt
//
LOCUS       DPUF01000027            4959 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_28240, whole
            genome shotgun sequence.
ACCESSION   DPUF01000027 DPUF01000000
VERSION     DPUF01000027.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_28240"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="DHV81_00915"
     CDS             complement(<1..896)
                     /locus_tag="DHV81_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HCI93108.1"
                     /translation="MKCILYKKALPLILLSCLLLGACGNSKSDDISKEPVSSHSTEKG
                     TWDSAPKVLTPTADGTQTYTCDVATIDASNSSSGYIIVNYHGSNQKVKLQITGPDSVT
                     YTFDLHGGNEVFPLVASSGSYTVTVFENVEGTQYATVLSQVISVSITDEFGPYLYPNQ
                     YVNFTADSPAVKEGSSLAYYCSSDTEVVESVYNYIISNFKYDYDKAKNVKSGYLPDVD
                     KVFTENTGICFDYAAVMATMLRTQAIPTRLEVGYAGEEYHAWISTYIKDKGWINGIIE
                     FDGTSWNMLDPTFASTSDSPKK"
     gene            965..1027
                     /locus_tag="DHV81_00920"
                     /pseudo
     CDS             965..1027
                     /locus_tag="DHV81_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscL"
     gene            1098..1337
                     /gene="secG"
                     /locus_tag="DHV81_00925"
     CDS             1098..1337
                     /gene="secG"
                     /locus_tag="DHV81_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCI93109.1"
                     /translation="MATLKIVLSVLFIIVSLVITIVILFQEGKSAGLGTIAGVADTYW
                     GKNKGRSKEGRLVKATIVCVVLFFIISIALNMGIW"
     assembly_gap    1388..1460
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1503..3629
                     /gene="rnr"
                     /locus_tag="DHV81_00930"
     CDS             1503..3629
                     /gene="rnr"
                     /locus_tag="DHV81_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCI93110.1"
                     /translation="MSNIDKYETRKKMVYDFMCDDMYVPMKIKELCIVLGVKKEDRPQ
                     LEQILLDLQAEGRITLSKRGKYSKSEIKKTVGVFTAHQRGFGFVTVEGEPDDIFIPAE
                     YVNGAMHMDTVEITISPVTTGRRKEGKVVSVIERGMKQVVCTYEASDNFGFAVPDNTR
                     FGTDIFIPKERSKGAMSGHKVVVEITSYGKKGKKPEGKVVEIIGHIDDPGTDILSIVK
                     AYDLPVDFSEKIMHQVQNVAKDVTPADMAGRMDLRDWMMVTIDGEDAKDLDDAVSLYM
                     DGDNYVLGVHIADVSNYVQEHSALDVEALKRGTSVYLVDRVIPMLPRELSNGICSLNE
                     GCDRLALSCIMTINKKGEVIDHKIAETVIKTNRRMTYTNVKKILADKDAAVIEEYKEL
                     VPMFEKMAELAAILRKKRMKRGSIDFDFPETKVVLDEDGHPIDIKPYDRNVATKLIED
                     FMLIANETVAEDYFWQEIPFVYRTHDKPDSEKIAKLSTFINNFGYTLHIGADEVHPKE
                     LQKLLMKVDGTDEESLISRLTLRSMKQARYTTACTGHFGLAANYYCHFTSPIRRYPDL
                     QIHRIIKENIRGRMNDNRREHYESILDAVAKQASETERRAEEAERETVKLKKCEYMSN
                     HIGECFEGVISGVTEWGFFVELPNTVEGLVRVTDLTDDFYEFYEDTYELAGSATNKRY
                     KLGQKIKVVVDSTDKIMRTIDFKPAE"
     gene            3861..4328
                     /locus_tag="DHV81_00935"
     CDS             3861..4328
                     /locus_tag="DHV81_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCI93111.1"
                     /translation="MAKEPKKLIANNKKVYHDYFLLEKYEAGISLAGTEVKSLRMGKG
                     SIKESFIRIENGEIILYGMHITPYEKGNIFNKDPLRPKKLLLHKKEIEKLSSKMKEKG
                     FTIVPVEVYFKGSLVKVEIALAKGKKLYDKRADIAKKDQKREAQRDFKIRNLG"
     gene            4462..4946
                     /gene="ssrA"
                     /locus_tag="DHV81_00940"
     tmRNA           4462..4946
                     /gene="ssrA"
                     /locus_tag="DHV81_00940"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     assembly_gap    4598..4691
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1518 a          836 c         1164 g         1274 t
ORIGIN      
        1 aacttctttg gcgaatcact ggtagatgca aatgttggat ccagcatgtt ccatgaggta
       61 ccatcaaact cgatgatgcc gtttatccag cccttgtcct ttatataagt actgatccag
      121 gcatgatatt cttcaccggc atatcctacc tctaaacgtg tgggaatagc ctgagtcctt
      181 agcatagtgg ccatgactgc cgcatagtca aagcatatac cggtattttc ggtaaagacc
      241 ttgtccacat caggcagata gccgcttttg acatttttag ccttgtcata gtcgtatttg
      301 aagtttgaaa tgatataatt gtatacactt tccacgacct cagtatcgct gctgcagtag
      361 tacgccagtg agctgccttc cttcacagcc ggagaatcgg cagtgaaatt tacatactgg
      421 ttagggtaca gatacggtcc gaattcatcg gtgatggaga cagaaatcac ctgcgacagc
      481 acggtggcat attgtgtccc ctcaacattt tcaaacacgg ttacagtata cgaaccactt
      541 gaagcaacca gaggaaacac ttcatttcct ccatgcagat caaaggtgta cgtgacactg
      601 tcaggcccgg ttatctgaag cttgaccttt tgattagaac cgtgataatt cactatgata
      661 tagccgctgc tactgtttga tgcgtcgata gtagccacat cacaggtata ggtttgtgtg
      721 ccgtcggcag ttggagtgag cacctttggt gcagagtccc aggtgccttt ttcagtagaa
      781 tgactggaaa caggctcctt tgatatatcg tcacttttgg aattaccgca tgcaccaagc
      841 aaaaggcatg ataataatat gagtggaagt gcttttttat acagtatgca cttcataaaa
      901 atctcctaaa tataaagttg tttctttttt aattatatta taatgtattt tcttgtaaaa
      961 taaaatggaa aaatgtataa tattaaaaaa tggtttagat tatcaaaaga agagagtaaa
     1021 aagaaaactc ttgactgtgc aataaaaacg tagtatatta gttctgttat ttagaattga
     1081 aattaggagg tcttatcgtg gcaactttaa aaattgtttt atcggtatta tttataatcg
     1141 tcagtttagt aattactata gttattcttt tccaggaggg caagtcagca gggcttggca
     1201 ctatcgccgg agttgctgat acatattggg gcaagaacaa gggacgttca aaggaaggac
     1261 gcctggttaa agcgacaatt gtatgtgttg ttcttttctt tattatttca attgctttaa
     1321 atatgggaat atggtaaata cttagactgt cgagaaatcg gcagtctttt ttatcgcatc
     1381 aacatccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn ctgtgcattt gaaatcagat ggggcaacga aaaggagaga
     1501 taatgagtaa tatagataaa tatgaaacac gaaaaaagat ggtctatgac ttcatgtgtg
     1561 atgacatgta tgttccgatg aaaatcaagg aactttgcat agtactagga gtaaaaaaag
     1621 aggacagacc tcagcttgaa cagattcttt tggatttgca ggcagaggga aggataacac
     1681 tctcaaagcg cgggaaatat tcaaagtcgg aaatcaaaaa gacagtcggc gtattcacag
     1741 cgcatcaaag gggatttggc tttgtcactg tggagggaga gcctgatgat atttttatcc
     1801 cggcagagta tgtaaatggt gcgatgcaca tggatactgt tgagattaca atctcacctg
     1861 tcacaacagg acgccgcaag gagggaaagg ttgtttccgt catagagcga ggcatgaagc
     1921 aggtagtctg cacctatgag gcaagtgata attttggttt tgcagtgcct gacaatacca
     1981 gatttggcac agatattttc ataccaaagg agcgctcaaa gggtgctatg tcgggccata
     2041 aggtagtggt tgaaatcaca agctatggta aaaagggtaa aaagccggag ggcaaggttg
     2101 ttgaaataat cggacatata gatgacccgg gtacagatat tctttctata gtaaaggctt
     2161 atgatctgcc ggtggatttt tcggagaaaa tcatgcatca ggtgcagaat gtggcaaagg
     2221 atgtgacacc tgcggatatg gccggcagga tggatttacg cgactggatg atggtcacga
     2281 tagacggaga agatgcaaag gatcttgatg atgccgtgtc tttatacatg gatggcgaca
     2341 attatgtact tggagtacac atagcagatg tgtcgaatta tgtgcaggag cacagtgccc
     2401 ttgatgtgga ggcacttaag agaggaacat ctgtttatct tgtagacaga gtgattccga
     2461 tgcttccaag agagctttca aatggcatat gctccttgaa tgagggatgt gacaggctgg
     2521 ctctttcatg cattatgaca ataaataaaa agggtgaagt tatcgatcat aagattgcag
     2581 agacagtcat aaagacaaac cgcaggatga catacacaaa tgtaaaaaag attcttgcag
     2641 ataaggatgc cgctgtgatt gaagaatata aggagcttgt gcctatgttt gaaaaaatgg
     2701 cagagcttgc agcaattcta cgcaaaaaga gaatgaaacg gggttctata gactttgact
     2761 ttccggagac taaagtcgtg ctcgatgaag atggtcatcc gatagatata aagccgtatg
     2821 acagaaatgt ggcaacaaag ctcattgagg attttatgct tatagcaaat gaaactgtgg
     2881 ctgaggatta tttctggcag gaaatacctt ttgtatacag aacacatgac aagccagact
     2941 cagaaaaaat cgcaaagctt tccacattta tcaataattt tggctatacg cttcatattg
     3001 gagctgatga ggtacaccca aaggagcttc agaagctttt gatgaaggtg gatggcacag
     3061 atgaagaatc cctgataagc agactgacac tgcgctctat gaaacaggca cgatatacaa
     3121 cagcatgtac aggacatttt ggactggcgg caaattatta ttgtcacttt acatcaccaa
     3181 tccgcagata tcctgatttg cagatacacc gtatcatcaa ggaaaatatc cgcggcagga
     3241 tgaatgataa ccgcagggag cactatgagt ctatactcga tgctgttgca aagcaggcaa
     3301 gtgagaccga acgacgtgca gaagaggcag agcgtgaaac agtcaagctt aaaaaatgtg
     3361 aatacatgtc aaatcatatt ggtgaatgct ttgaaggagt tatatcaggt gtcacggagt
     3421 ggggcttttt cgtggagctt ccaaatacag ttgagggact tgtgcgcgtg acagacctga
     3481 cagatgattt ctatgagttt tacgaggata cctatgagct agccggttca gccaccaata
     3541 agagatacaa gcttggacaa aaaatcaagg ttgttgtgga cagtactgac aaaattatgc
     3601 gtacaattga ttttaaaccg gcggaataat atgacaataa tgacgaaatt ttttatgata
     3661 ttaaaaatac ttatttgatt atctgttatt tgtcggtcgc tgtgcgatag atttattgaa
     3721 aaagatgtca taatatatta tatagtgtaa ataaacagaa atgtgaacca gcggtttaca
     3781 ataatttgcg ttttatatat tggcaattgt gaaaaaatgt gttagtattg taaagcagtt
     3841 tgaggaaagg agataccgac atggctaagg aaccaaagaa attaatagcc aacaacaaaa
     3901 aggtctatca cgattacttt ttgcttgaga aatacgaagc cggtatttca cttgcaggaa
     3961 ctgaggtgaa gtcacttcga atgggaaagg gcagcataaa ggagtcattc atccgtattg
     4021 agaacggtga gattatcctg tatggcatgc atatcacacc atatgagaag ggaaatatct
     4081 tcaacaagga tccactcaga cctaaaaagc ttttgctcca caagaaggaa atcgagaagc
     4141 tttcatcaaa gatgaaggaa aagggtttca caattgtgcc ggtggaggta tactttaagg
     4201 ggagtctggt caaagtagag atagcacttg ccaaaggtaa gaagctttat gataagagag
     4261 cagacatagc caagaaggat caaaagcgag aggcacagag agatttcaaa atcagaaatc
     4321 ttggttagca aaaaatgaat atgggctagt aaaggtttcg acggggccca caaggtccgg
     4381 tggaccttgg ctttgggcgg accgaagcgg agcggagaac atggagcgta gctgcatgta
     4441 ccccgcaaaa aaattgaata tgggctagta aaggtttcga cggggtccac aaggtccggt
     4501 ggaccttggt tttgggcgga ccgaagcgga gcggagaaca tggagcgtag ctgcatgtac
     4561 cccgcaaaaa aaaatgaata tgggctagta aaggtttnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn ngctgaagat aatttagcat acgctgccta atggcagcag tctgacctag
     4741 agcactcaca ctttaggacc caggcttcga ctatgtgaga aacgacgttg gcaaagcttt
     4801 gagccatcgg gcgtatcatg aagctactga aaccacaagg ttgtttgtta tcgtgtggca
     4861 gagggaatgt taaataataa actacactcg tagaaggaca gggaattgta tttcggacac
     4921 gggttcgact cccgtctagt ccactaaaag gcggtttgt
//
LOCUS       DPUF01000028           14824 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7279, whole
            genome shotgun sequence.
ACCESSION   DPUF01000028 DPUF01000000
VERSION     DPUF01000028.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 14824)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14824)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14824
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7279"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="DHV81_00945"
     CDS             complement(<1..1547)
                     /locus_tag="DHV81_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93112.1"
                     /translation="MLYQIKDGTVSAGGQTILSHVDFYIKEKEKIAVVGKNGAGKTTL
                     LRLLAGELTPDRDDSRGSYGRSNDMVTGAATAGSDLDGTAKRTQRAKKKKPSGNPETG
                     ITMSRNITIDMLRQADKSNQDLTIEQILLESCPDKDTFSKERFDYEMEYDRLFTGFGF
                     EKSDKTRLFRSFSGGEQTKISLIKLLLKKPDLLLLDEPTNHLDMKTVEWLEDYLINYP
                     KAVVMVSHDRAFLDAVATGVYELENGALHRYAGNYTQYRQQKLKNLQIQRKAYERQQA
                     EIAHNNELIDKFKHKPKKAAFARSRKTMLARMKLIEKPVEDEAHIFTGNIEPQFPGGK
                     WVYEAKELKIGYDGRALLELSLRIRRGQKIAVIGDNGIGKSTFLKTVAGLIPPIKGTS
                     QLGSNLLVGYFDQQSALIDSEKTVRDHFHELFPALVEKELRKTLGMYLFGGANASKRI
                     SSLSGGEKSRLVLAELLTGRPNLMILDEPTNHMDIPAKETLESAFKAYTGTMLFVSHD
                     RYFIKQVA"
     gene            complement(1557..1781)
                     /locus_tag="DHV81_00950"
     CDS             complement(1557..1781)
                     /locus_tag="DHV81_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93113.1"
                     /translation="MNPASIMKMMKEKNTFIANHPKFVSFLQYAFGSGIPADSVIEIT
                     VTKPGQEPVTSNIKVQQSDLELLESLKDLK"
     gene            complement(2046..2891)
                     /locus_tag="DHV81_00955"
     CDS             complement(2046..2891)
                     /locus_tag="DHV81_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI93114.1"
                     /translation="MKPKHKINVRSVIAHIVLILLSFLCLFFFVILIINATRSHAQLQ
                     KGFSFIPGGHFLDNLKSVANDGSFPMFRGIVNSLIISGSCAVLCTYFSSLTAYGLYAY
                     DFKLKKAAFTFIMAILVMPTQVTAMGFLRLITNMGMYDSWTPLIIPSIASPAVFYFMY
                     SYLQSSLPLSLVEAARIDGSNEFRTFNHIVLPIMKPAIAVQAIFTFVGSWNNYFTPAL
                     IIQSKNKMTVPILIATLRGADYMNFDMGKIYMMITVAIVPIIVVYLLLSKYIIAGVTL
                     GGVKE"
     gene            complement(2891..3862)
                     /locus_tag="DHV81_00960"
     CDS             complement(2891..3862)
                     /locus_tag="DHV81_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI93115.1"
                     /translation="MKKKRKSVSYAKWGYIFILPFFITYLIFSLIPLVDTVRFSFFEY
                     YRSGIKQIGPNFVGWANYAELLKSDMITYGKNTLILWVIGFIPQIVVALVLANWFTDA
                     RLKIHKLQFFKVVIYLPNLIMASAFALLFFTLFSNSGPVNSILQSMGVISKPIDFMGS
                     VFGTRSLIGFMNFLMWFGNTTIMLMAAVMGISTEVFEAADLDGCNALQRFFYITLPSI
                     RPILAYTLITSIIGGLQMFDVPQILTNGQGNPDRTSMTLIMFLNNHLKSKNYGMAGTL
                     SVYLFIVSAILCFIVYRMTNDSDPDGSKAAAKRAAKEARRKKKEAAK"
     gene            complement(3932..5317)
                     /locus_tag="DHV81_00965"
     CDS             complement(3932..5317)
                     /locus_tag="DHV81_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI93116.1"
                     /translation="MKQKVISTMLLLSMGATLLAGCGTTAGNGTEGGASNAAEGKVIN
                     IYSWNDEFRTRVEAVYPEVKETSKDGTITYLKDGTEIHWVVNPNQDGVYQQKLDEALS
                     KQADAAADDKIDMFLSETDYVFKYTDKDADVAMPLKDLGIDCDKEFADQFDFTRKTAS
                     DSDGVQRGSTWQCCPGLLVYRRDIAKDVFGTDDPEAIGEKTKDWDTLKATAAELKAKG
                     YYTLSSYADTFRLYGNSIDNSWVQPGADEVVVDKKITNWVDDSKEWLDAGYVDAKVKG
                     QFNDDWNKAMGSQSKVFAFLFPAWGIDFTLAPNWDGAEGSWAVTTPPQEYNWGGSYIH
                     AATGTDNPKHVKDIILALTANKDNLLKISKDYSDFTNTKSGMKEAAKDDSFASDFLGG
                     QNPFAYFEPVAENIKIAPLSAYDQGCVELIQNSFSDYLQGKVDYDKAKKNFETAIKER
                     YPDIEKVTWPK"
     gene            5506..6246
                     /locus_tag="DHV81_00970"
     CDS             5506..6246
                     /locus_tag="DHV81_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI93117.1"
                     /translation="MALSKEWYDAEFVDSESESLHRAPSIEYSFYNAVKTGDMEYVIK
                     NCKEDAFIHLEGTGVLSRNTLQNIKYHFVVTTAMITRYCIDGGLEPEQAYRLSDFYIL
                     KMDSCSTVHQVADLHHEMAKDFTGKMVLQKKSSILSKPVTQCVDYIYSHIKERVTIAD
                     LAAYTGLSESYLSRVFKQNLGVSISDYIREKKIEKATHLLKYSDKPIIDIANYLSFSS
                     QSHFIQIFKSFTGLTPKKYRDRYYKSMW"
     gene            complement(6363..9065)
                     /locus_tag="DHV81_00975"
     CDS             complement(6363..9065)
                     /locus_tag="DHV81_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellobiose phosphorylase"
                     /protein_id="HCI93118.1"
                     /translation="MKNIDFIDKYGTFRIKNPENYSGLYLPLAGEKGLKSSITPTLGG
                     DSKTSQNTFLLEPVSIENLHNNKGTRNFWCRIVGKGFWSVCGSSAQQEADRFTGNQDE
                     SELEAGLMWQKLHRASKIYGLEADTTSFVTLDGSMEVMLVEITNKTDEAMEIVPIGAI
                     PIYGRSADNIRDHRHVTSLLHRIETTQYGIEVTPVLSFDERGHRKNDISYFVYGSSGN
                     GESPESFYPTVEQFIGEGGSFLIPEAVRTEKAGAKAGEKFQGKEAVGAIKFANRIIKP
                     LETVSYIMLAGLTEKENDINAITGRYRSVLEVKEELNTVKKHWIDKVNIDFETGNAKE
                     DNYLKWICFQPILRRIYGCSFLPHHDYGKGGRGWRDLWQDCLALLLMEPSDVRRMIVN
                     NYGGVRIDGTNATIIGNEPGEFIADRNNITRVWMDHAFWPFVTTKLYIDQTGDTDVLF
                     ERTTYFKDYQSMRGTSHDEAWNNTYGNKQRTIGSQIYFGTVLEHILIQNLCAFYDVGE
                     HNEMKLHGADWNDALDMAWDKGESVAFTCAYAGNLLDIAKCLRNVEKISGINRIEVLE
                     ELKLLLADDEILYNCPDKKQELLMSYAKACENCTSGGTVLVPIAAICENLEHKAEWMM
                     KNIRENEWISDGTDGGWFNGYYDNNGRPVERCESGDVRMMLTGQVFAIMSGTAKKEQI
                     KAICNSADKYLFDQKAGGYRLNTDFKEEKFDLGRMFGFAYGEKENGAVFSHMAVMYAN
                     ALYKQGFIKEGYKVLKTLLDTAMDFDRSRMYPGVPEYFDNDGRGLYSYLTGAASWYML
                     TMITSVYGVHGELGDLVIEPALMPQQYNEKGDAKVSLEFAGHGFDILVHNPDKLEPGD
                     AHVKRALCDDVKVENVTGNSVRITKHAIEMFSTDKCHTVEIYIE"
     gene            complement(9156..11567)
                     /locus_tag="DHV81_00980"
     CDS             complement(9156..11567)
                     /locus_tag="DHV81_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HCI93119.1"
                     /translation="MKYGYFDESKKEYVITRPDTPAPWVNYLGSPEYGAIVSNNAGGY
                     SFAKSGANGRLLRYVFNQFDEPGRYIYIRDNGSKDYWSASWQPVGKDLNEYKSECHHG
                     TAYTKMMADYSGIHSEVRYYVPLNKTYEVWNLSVTNNSDKARSLNITGYAEFTNNSNY
                     EQDQVNLQYSQYITKTVFVENRVRQMIHANLDRIENGKEIDNKDVVNRFIGLAGAPVD
                     SWCGDRGEFLGEYHRYGNPVGVESGKLNNHGNYNENSCGAITTVLELAPGETKTIAFL
                     VGMIDNETAGKIVASYTDTKAVCDKELEELIAYWHGQLSHFQINTPSDEFNTMINTWN
                     AYNCFMTFIWSRAASFTYCGLRNGYGYRDTVQDIQGVIHLAPEMAVEKIRFMLSAQVD
                     NGGGLPLVKFTHNPGHEDTPDDASYVQETGHPAYRADDALWLFPTVYKYVSETGNVAF
                     IDEVIPFANKDEGTVYEHLKRAIDFSMNHLGKHGMPAGLYADWNDCLRLGADGESTFV
                     ALQFYYAMTILKEFAAYKKDDEYIAYLDESQEKLGKIIQELCWNEDRFIRGFTGDGQV
                     IGKRDDPEANMWLNPQSWAVISGFASDEQADKALEMVYERLNTEYGAILMDPPYHAHA
                     FDGALAVIYNAGTKENAGIFSQSQGWIILAEALKGHGDRAFKYFIENAPAAQNDRAEI
                     RRLEPYCYGQFTEGKASPNFGRSHVHWLTGTASTVMVGCVEGILGMRPDFYGLHIAPS
                     IPKAWDGFEIEKDFRGCHLHIVVKNPDHVESGCKSLLVNGQAVEGDYIPKELLSEQTE
                     IELTM"
     gene            complement(11712..12292)
                     /locus_tag="DHV81_00985"
                     /pseudo
     CDS             complement(11712..12292)
                     /locus_tag="DHV81_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12445..13275)
                     /locus_tag="DHV81_00990"
     CDS             complement(12445..13275)
                     /locus_tag="DHV81_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI93120.1"
                     /translation="MQQYTHENVRSIPHLPIYCDRYEDYRQFSPRHWHDHIEIIYVVS
                     GSLQVVCGENEYTLQENEFFVINSNKIHGTQSYERVKVLLVQIPYVYLDSYIDDYGHI
                     RFRECYETEHGSRKYEQMKSLLKSIAHIYRKKGKGYELRLMAKVNEFLYILFTNYSYK
                     EMNAGKESRQIARLKDVLRYVAKNYQEPIALKEAADIASLNQDYFCRMFKKCMGVTFL
                     EYVNQVRINHIHEELLATEDSITDILEHNGFTNYKVFSRMFKAQFGMTPRELRKLQEN
                     "
     gene            13590..>14824
                     /locus_tag="DHV81_00995"
     CDS             13590..>14824
                     /locus_tag="DHV81_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HCI93121.1"
                     /translation="MNKWSRFKFTPNLPLGANGERVTGSKAHIELSKEAAKEGMVLLK
                     NENNVLPLAAGSKVALFGKGTFDYVKGGGGSGDVTVAYIRNLYEGLKLQKEKISIFEE
                     LCDYYRNDIKKQYAAGAVPGMTIEPDVPAELLSKAQAYTDTAIISICRYSGEGWDRKS
                     VIDPNNKALWDYEREMTEKSAELFKDGDFCLSVKEKEMIDTVKASFKNVIVILNVGGM
                     VDTSWFAYDNQIQSALLALQGGMEGGLAAAELLVGDGNPSGKTVDTFAKSLDDYPSTY
                     NFHESRDYVDYTDDIYVGYRYFETIPGAAEKVVYPFGYGLSYTTFDVETVSAGVVNSN
                     CTSEANKLYAKVRVTNTGKFSGKEVVQVYIAKPQGKLGKPAKELAAFEKTRELQPGES
                     QLMILTWEINDMASYDDLG"
BASE COUNT         4013 a         3465 c         2705 g         4641 t
ORIGIN      
        1 gccacctgct ttatgaaata cctgtcatgc gatacaaaga gcatggtgcc ggtatatgcc
       61 ttaaatgccg attctagtgt ctccttcgcc ggaatatcca tgtgattggt cggctcatcg
      121 agtatcatta agttcggacg gcctgtcaga agctctgcga gcacaagcct ggacttctca
      181 ccgcctgaga gcgagctgat acgctttgag gcatttgccc ctccaaacag atacatgcca
      241 agcgtctttc ttaattcctt ctccacaagt gccggaaaaa gctcgtgaaa atggtctcgc
      301 acagtctttt cgctgtcgat aagtgccgac tgctggtcaa aatagcccac cagcagattg
      361 ctgccaagct ggcttgtgcc ctttattggt ggaataagcc ctgccacagt ctttagaaat
      421 gttgattttc ctatgccgtt gtcaccgatt acggcaatct tctgaccacg ccttatacga
      481 agcgacagct caagaagtgc cctgccgtca tatccaattt ttaattcctt cgcctcgtaa
      541 acccatttac cgcccggaaa ctgtggctcg atattgcctg taaaaatatg agcttcgtct
      601 tcaaccggct tttctatcag cttcatgcgt gccagcatgg tctttctaga gcgtgcaaac
      661 gcagctttct taggcttatg cttgaatttg tcgataagct cattattgtg cgcaatctcc
      721 gcctgctggc gctcgtaggc tttgcgctgt atctgcagat ttttaagctt ctgctgcctg
      781 tactgagtgt agttgccggc atatctgtgc aatgctccgt tttcaagctc atatacacca
      841 gtcgcaactg catcaagaaa tgccctgtca tgggacacca taactacagc ctttggatag
      901 ttaatcagat aatcctccag ccactccacc gttttcatgt ccaaatgatt agtcggctca
      961 tccaagagca aaagatcagg cttctttaaa agcagcttta tcagagaaat cttcgtctgt
     1021 tctcctcctg aaaagctcct aaaaagcctc gtcttgtcac tcttttcaaa gccaaagcct
     1081 gtaaaaagcc ggtcgtactc catctcgtag tcaaacctct cttttgaaaa ggtatcttta
     1141 tccgggcagc tctcaagcag tatctgctct attgtcaaat cctgatttga tttatccgcc
     1201 tgtctaagca tatcaattgt gatatttctg gacatggtaa ttccggtttc cgggtttccc
     1261 gacggtttct tcttttttgc tctttgagtt cttttcgccg taccatctaa atcactgcct
     1321 gcagtggcag ctcccgtgac catatcattg cttctgccgt agctgcccct gctgtcatcc
     1381 ctgtccggtg tcagctctcc agcaagaagc cgaagcagcg tggtctttcc agcaccgttt
     1441 ttgccgacta cagctatttt ttctttttcc tttatataaa aatcaacatg ggacagaatc
     1501 gtctgacctc ctgccgaaac tgtcccatct ttaatctggt ataacatatt ttacctctat
     1561 tttaagtcct taagactctc caaaagctcg agatctgact gctgcacctt gatattggag
     1621 gtcacaggct cctgtccagg ctttgtaact gtgatttcaa taacagagtc tgccgggatg
     1681 ccgctgccga atgcatactg taaaaaggat acaaactttg ggtggtttgc tataaatgta
     1741 tttttttctt tcatcatttt cattatgctt gctgggttca tgtattttct ccaatcttat
     1801 ctatatgata tctctttcga cgcacatatc tttacgttcg ttatattata ccatgagtta
     1861 attctataat ctatcatttt agaattattt aatcaacaat catctgctga tatactgcat
     1921 atgtgccaca atatttccta aataaaaaat atgccatacc tcatatttca atatactaaa
     1981 aaacagatga cccacataac atatatgtaa gccatctgct ctgatgctaa aactaattaa
     2041 atcgtttact ccttcacgcc tccaagtgtg acaccggcaa taatatattt tgataagagg
     2101 agatatacaa caataatagg cacgatagct actgttatca tcatatagat ttttcccata
     2161 tcgaaattca tatagtccgc tcctcgaagt gtggcaatca aaattggtac tgtcatcttg
     2221 ttctttgact ggatgatcag ggccggtgtg aagtagttgt tccatgaacc tacaaatgtg
     2281 aaaattgcct gtactgcaat tgcaggtttc atgatcggca atactatatg gttgaatgtc
     2341 ctaaactcat tcgaaccatc aattcgtgca gcttctacaa gtgatagagg aagtgatgac
     2401 tgtagatagc tgtacatgaa atagaatacc gccggcgatg ctattgatgg aatgataagt
     2461 ggagtccacg aatcatacat tcccatattt gttattaaac gcaggaagcc catcgcagta
     2521 acctgtgtcg gcataaccag tatcgccatg ataaatgtaa atgcagcttt ctttaattta
     2581 aaatcataag cataaagtcc atatgctgta agcgatgaaa aatatgtaca taaaactgca
     2641 catgagcccg atataataag gctgtttact attcccctaa acatagggaa ggatccgtca
     2701 ttagctacag atttcagatt atccaggaaa tgcccgcccg ggataaatga gaagcccttc
     2761 tgcagctgtg catgcgacct tgtcgcattg attataagta ttacaaagaa gaacaggcat
     2821 aaaaatgata ataatatcaa aactatatgc gctataacac tgcggacatt aattttatgt
     2881 tttggtttca ttatttagcc gcctcctttt tctttcttct agcttctttt gcagctcgct
     2941 ttgctgctgc ctttgatccg tcaggatcgc tgtcatttgt cattctgtat acgatgaagc
     3001 acagaattgc gctgactata aacagatata ccgacaatgt tccggccata ccatagttct
     3061 tactctttaa gtggttgttc aggaacatga taagtgtcat tgacgttctg tccggatttc
     3121 cctgtccgtt tgtcagaatc tgaggtacat cgaacatctg gagtccaccg atgattgatg
     3181 tgatgagtgt atatgcaagt attggtcgta tactcggtaa agttatatag aagaatctct
     3241 gcaatgcatt acatccatcc agatctgccg cctcaaatac ttctgtactg atacccatga
     3301 ctgctgccat gagcataata gtcgtattac cgaaccacat aaggaagttc atgaagccga
     3361 tgagtgatct ggttccaaat acacttccca taaagtcgat aggcttgctg attactccca
     3421 tagactgcag gattgagttc acaggaccac tgttcgagaa cagtgtaaag aacaaaagtg
     3481 caaatgcaga tgccatgatc aggtttggca gataaattac taccttaaaa aactgtaatt
     3541 tgtgaatttt taatctggca tcagtgaacc agtttgctaa aacaagtgca accacaatct
     3601 gtggaataaa tccgataacc cagaggataa gagtattctt accatatgtg atcatatccg
     3661 atttaagaag ctcggcatag ttagcccatc ccacaaaatt cggtcctatt tgtttgattc
     3721 cggatctata gtattcaaaa aaactaaacc ttaccgtatc tactaacgga atcagagaaa
     3781 aaattaaata tgttatgaaa aacggaagaa taaagatata tccccatttt gcatagctta
     3841 cgctctttct tttcttcttc atgagccaaa ttcctttcta ccagagtcac ctctgcgaag
     3901 cccaaaggct ccgcatggtg ttttatttat cttatttcgg ccatgtaacc ttttcgatat
     3961 caggataacg ctctttaatt gctgtctcaa agtttttctt agccttatca taatcaacct
     4021 taccctgtaa gtaatcgctg aatgagttct ggataagctc tacacatccc tgatcatatg
     4081 ctgaaagagg tgcaatcttg atattctcag ctaccggttc aaagtaagca aatgggttct
     4141 gtccgccaag gaagtctgat gcgaatgaat catcttttgc tgcttccttc attcctgatt
     4201 ttgtatttgt gaaatcagag taatccttag agatcttgag taagttgtct ttatttgctg
     4261 ttaaagcaag gatgatatcc ttaacatgct tagggttatc tgttcctgtt gctgcgtgga
     4321 tgtaagatcc accccagtta tactcctgtg gtggtgttgt aacagcccat gatccttctg
     4381 ctccatccca gtttggtgca agtgtgaaat cgattcccca tgctgggaaa aggaatgcaa
     4441 atacctttga ctgagaaccc attgctttgt tccagtcatc attaaactgg cctttaactt
     4501 tagcatcaac atatcctgca tcaagccact cttttgaatc atctacccag tttgtaatct
     4561 tcttatccac tacaacctca tctgctccag gctgtaccca tgagttgtcg attgagtttc
     4621 catataaacg gaatgtatca gcgtaagaag ataatgtgta gtatcccttt gccttaagtt
     4681 ccgctgctgt agcctttaat gtatcccagt cctttgtctt ctcaccgatt gcttcaggat
     4741 catctgttcc gaatacatcc tttgcaatat cgcgacggta aactaaaagt ccaggacagc
     4801 actgccatgt tgaacctctc tgtactccgt ctgaatcaga agctgttttt cttgtgaaat
     4861 cgaactgatc agcaaactct ttgtcgcaat caattccaag atccttaaga ggcattgcta
     4921 cgtctgcatc tttatctgta tatttgaata cgtaatctgt ctctgataag aacatatcaa
     4981 tcttgtcatc tgctgctgca tccgcctgtt ttgaaagagc ttcatcaagc ttctgctggt
     5041 aaacaccatc ctggttagga tttactaccc aatggatttc tgtgccatcc ttgaggtatg
     5101 taattgtacc atctttagaa gtttctttaa cctccgggta aacagcctca actcttgttc
     5161 gaaactcgtc gttccatgaa tagatattga taacctttcc ctctgctgca ttagaagctc
     5221 caccttctgt tccgtttcct gctgttgttc cacatcctgc aaggagtgtt gcccccatag
     5281 acagtaacag cattgtgcta atcactttct gtttcatttt tcatttcctc catagaaact
     5341 gttttgatat cttcttatga attaatttga tatctcgttt acagttgtaa tattactcct
     5401 taaccgtttt cgtttatatt aaatcaattg taaaaatatc agattcatgt cataataata
     5461 gaaagatgac ataaatctaa tattttgttg caaaggagaa cctttatggc actttcaaaa
     5521 gaatggtatg acgcagaatt tgttgacagc gagagcgaat cactgcatcg tgctccaagt
     5581 attgaatata gtttttataa cgcagtcaaa accggggata tggaatatgt aataaaaaac
     5641 tgcaaagaag atgcatttat acaccttgaa ggaacaggtg tattgtccag aaatacttta
     5701 cagaatatca aatatcactt tgttgtcaca accgccatga tcacaagata ctgtattgat
     5761 ggaggactcg agcctgagca ggcataccgt ctgagtgact tctatatact taagatggac
     5821 agctgctcca ccgtacacca ggttgctgac ctgcatcacg agatggcaaa ggatttcaca
     5881 ggtaagatgg tgctacagaa gaaatcgtct atcctctcaa agcctgttac ccagtgtgtt
     5941 gactatatat acagccacat aaaagagcgt gtcacaatag ctgatctggc tgcatacaca
     6001 ggtctttccg agagctatct ttcaagggta ttcaagcaga atctcggtgt ttcgataagt
     6061 gattatataa gggaaaagaa aatagaaaaa gcgactcacc tgctcaaata ctcagataag
     6121 ccgattatag atatagccaa ctacctgtca ttttcatcac agagccactt tatccagata
     6181 ttcaagagtt ttacagggct tacaccaaag aaatatcgcg acaggtacta caaatccatg
     6241 tggtagtctg ataccaaaag ccttgtacta cgcacaaaaa attcccacgc aaacatgcgt
     6301 gggaatttta tttagctgat acccaatgat acgatatcaa acgctattaa ttttataaac
     6361 tatcactcta tgtatatctc aaccgtatga catttgtctg tagaaaacat ttcaatagca
     6421 tgctttgtaa tacgcacgct gtttcctgta acattttcca cttttacatc atcacagagc
     6481 gctcttttga cgtgcgcatc cccaggttcg agcttatcag gattgtgaac taaaatatca
     6541 aatccatgac ctgcaaattc taacgaaact tttgcatcac ctttttcgtt atactgctgt
     6601 ggcatgagtg caggctcaat aacaagatct ccaagctctc catggactcc gtatacagat
     6661 gttatcatag taagcatata ccagcttgcg gcacctgtaa ggtatgaata cagacctctt
     6721 ccatcattat caaaatactc cggtactccc ggatacatcc tgcttctgtc aaagtccatc
     6781 gccgtatcaa gaagtgtctt taataccttg taaccctctt ttataaagcc ctgtttatag
     6841 agcgcattgg catacattac agccatatgt gagaatacag caccattttc tttttcacca
     6901 tatgcaaatc caaacattct tccaagatca aacttttctt ccttaaaatc agtgtttagt
     6961 ctgtagccac cggctttttg atcaaacaga tatttgtctg cgctattaca gattgccttg
     7021 atctgttctt tttttgcggt tccactcatg atggcaaata cctgtcctgt aagcatcatg
     7081 cgtacatctc cactctcaca tcgttccacc ggtctgccgt tattgtcata atatccgttg
     7141 aaccagcctc catccgtgcc atctgatatc cattcgtttt cgcggatgtt tttcatcatc
     7201 cactcagcct tatgctccag gttttcgcaa atcgcagcta ttgggacaag aacagtgcct
     7261 ccgcttgtgc agttctcaca tgcctttgca tagctcatga gaagctcctg ctttttgtca
     7321 gggcagttgt aaagtatttc gtcatcagca agcagcagct taagctcctc taacacctca
     7381 atcctgttta ttccgcttat tttttccaca tttcttaaac actttgctat atcaagcaga
     7441 tttccggcat atgcacatgt aaatgcaaca ctttcgcctt tgtcccatgc catatcgagc
     7501 gcatcgttcc agtctgcacc gtgcagcttc atctcgttgt gctcccccac atcatagaaa
     7561 gcgcacagat tttggatcag aatatgctct aaaaccgttc caaaatatat ctgactgcct
     7621 atcgtccttt gtttatttcc atatgtatta ttccatgctt catcatggct tgtgcctctc
     7681 atggactggt aatccttaaa gtacgtcgta cgctcaaaaa gtacatctgt atcaccggtc
     7741 tggtcaatat aaagctttgt ggttacaaat ggccagaatg cgtgatccat ccatacacgt
     7801 gtgatgttat ttctgtcagc aataaactcg ccaggctcgt ttcctataat agtcgcattg
     7861 gttccatcaa ttctcacacc gccataattg tttacaatca tcctgcggac atccgaaggc
     7921 tccataagta aaagtgcaag acagtcctgc catagatctc tccagcctct tccacctttt
     7981 ccataatcat gatgtggaag gaatgaacag ccgtatattc ttcgtaagat aggttgaaaa
     8041 catatccatt taagataatt gtcctccttg gcatttcctg tctcaaaatc aatatttacc
     8101 ttgtctatcc agtgtttctt gacggtgttt agttcttctt ttacttcaag tacactgcgg
     8161 taccgtccgg taatggcatt tatgtcattt tctttttccg taagacccgc aagcatgata
     8221 tagcttaccg tctccagcgg ttttataata cggtttgcaa attttattgc tccgactgct
     8281 tcttttccct gaaatttttc tccggccttt gcacctgctt tttcagttct gactgcctca
     8341 ggaataagga aagaacctcc ctctcctata aactgctcaa cggtaggata gaaactctca
     8401 ggactttctc cgtttccgga tgatccatat acgaagtagc ttatatcatt tttccggtgt
     8461 ccacgctcat caaatgagag gacaggtgta acctcaatgc cgtattgtgt ggtttctatt
     8521 ctgtgcaaaa gtgatgtcac atgtctgtga tccctgatat tgtcagcact tctgccatag
     8581 attgggattg ccccgattgg gactatctcc atagcctcat ctgtcttgtt agtgatctcc
     8641 acaagcatga cttccatact tccatcaagt gtgacaaatg atgtcgtgtc ggcctctaac
     8701 ccatatatct ttgaagcacg gtgcagcttc tgccacatta aacctgcctc aagctcactt
     8761 tcatcctgat tacctgtaaa tctgtcggcc tcctgctgtg cagatgatcc gcatactgac
     8821 cagaagccct ttcccacaat ccgacaccag aaatttctgg tgcctttatt gttgtggaga
     8881 ttttctatac taacaggttc cagaagaaag gtattctggc ttgttttact gtctccgccg
     8941 agagttggtg taatgcttga tttaagccct ttttcccctg caagcggcag atacagtcca
     9001 ctgtaatttt cgggattttt gatcctgaag gttccgtatt tgtctataaa atctatattt
     9061 ttcatatttt caatcaatcc tctatacaat tacaaatcaa attttcacac accatataca
     9121 atcctatagc agtcattatc ccacctacgg aaaatttaca tggtaagctc aatctctgtc
     9181 tgctcgctaa gaagctcttt tggaatatag tctccctcaa ccgcctgtcc gtttacaaga
     9241 aggctcttgc atccgctctc aacatgatca ggatttttta caactatatg aagatggcat
     9301 cccctgaaat ccttctctat ctcaaatcca tcccaggctt ttggaataga cggagcaata
     9361 tgaagtccat agaaatcagg acgcatacca agtattccct ctacacatcc aaccatgact
     9421 gttgaagcag ttcctgtgag ccagtgtaca tgtgaacgtc caaagtttgg acttgctttt
     9481 ccttcagtaa actgtccata gcagtacggc tcaagtcttc ttatctccgc tctgtcattc
     9541 tgtgcggcag gagcattttc aataaaatac ttaaaagctc tgtctccatg tcctttaagt
     9601 gcctcagcaa gaattatcca gccctgtgac tgtgagaata ttcctgcatt ctctttagtt
     9661 cccgcattgt atatcactgc gagtgctccg tcgaatgcgt gagcatgata tggaggatcc
     9721 ataagaatgg cgccatactc ggtattgagt ctttcatata ccatttcaag agccttatca
     9781 gcctgctcat cacttgcaaa tccacttata acagcccagc tctgtgggtt gagccacata
     9841 tttgcctcag gatcatcacg ctttccgata acctgtccat cccctgtaaa tccacggata
     9901 aatctgtcct cgttccagca aagctcctga atgatctttc cgagtttttc ctggctctca
     9961 tcaagatatg cgatatattc atcatctttc ttataggctg caaactcttt taatatagtc
    10021 attgcatagt agaactgaag tgctacgaag gttgactctc catctgcacc aagcctcagg
    10081 cagtcattcc agtctgcgta aagtcctgca ggcattccat gttttccgag gtggttcatc
    10141 gaaaaatcaa ttgctctctt taaatgctcg taaaccgttc cctcatcctt gttggcgaat
    10201 ggaatgacct catctataaa tgctacattt cctgtctctg atacatattt gtatacagtt
    10261 gggaaaagcc ataatgcatc atcagctctg taagccggat gtcctgtctc ctgtacatag
    10321 gaagcgtcgt caggagtatc ctcatgtccc ggattgtgtg taaacttgac aagcggaagt
    10381 cctccaccgt tatcaacctg tgctgagagc atgaatctga tcttttcaac agccatctca
    10441 ggtgcaaggt gtataacacc ctgaatatcc tggactgtat cacggtatcc gtatccgttt
    10501 ctgagtccac agtatgtaaa tgatgcagcc cttgaccaga taaatgtcat gaaacagtta
    10561 tatgcgttcc atgtattgat cattgtgttg aactcatcac ttggtgtatt aatctggaaa
    10621 tgtgataact gtccatgcca gtatgcgata agctcctcaa gttctttgtc gcagacagct
    10681 tttgtatccg tgtaagaagc aacaatcttt cctgctgtct cattatctat cattccaact
    10741 aagaatgcta ttgtctttgt ctcgcccggt gccagctcca atactgttgt gattgctcca
    10801 cagctattct cgttgtagtt gccgtggttg ttaagctttc cgctctctac tccgacagga
    10861 ttgccatatc tgtgatattc tccgagaaat tctcccctgt caccgcacca gctgtcaaca
    10921 ggtgctcctg caagtccgat aaatctgttt accacatcct tgttgtcaat ttccttgcca
    10981 ttctcgatgc ggtcaagatt ggcatggatc atctgacgca ctctgttctc cacaaatact
    11041 gtctttgtaa tatactgtga atactgcaga ttcacctgat cctgctcata gttgctgttg
    11101 tttgtaaact cagcgtagcc tgtaatatta aggcttcttg ccttatcaga attatttgtc
    11161 acgctcaaat tccacacttc gtatgtcttg ttcagcggaa cgtaatatct tacttctgaa
    11221 tgaataccgc tgtaatcagc catcatcttt gtgtatgctg ttccatgatg acactcactc
    11281 ttgtattcat tcaaatcctt tcctacaggc tgccacgaag ctgaccagta atccttgctg
    11341 ccgttgtctc tgatataaat atatctgcca ggctcatcga actgattaaa cacatatctc
    11401 aaaagccttc cgtttgcacc cgactttgca aagctgtatc cacctgcatt attggaaaca
    11461 attgctccat actccggcga tccaagatag tttacccatg gtgccggtgt gtcaggtctt
    11521 gtgataacat attctttttt tgattcatca aaatatccgt atttcatatt acttactcca
    11581 ttctctactt aattatgtga tctacggtta taactgcctc caacgtatat tcagcagtaa
    11641 tccattttcg taatataata tacatttttg cagcagtgta ttatacaaaa ttatttctta
    11701 aaatatcaga ttcatgacat aactaaactt attttaccgg gacgtttaac atcttcatcg
    11761 tagctatcct tctggtatac acggacataa tcgatcacat atggattatt ctcaaagctt
    11821 gcctcatcgt ttggatttcc aacccagctt ccaccaacag caagatttaa gataatgtaa
    11881 atgcaggaag gtatccatgt ccctttggaa cctttgcacg gcattcaaat cgtccatata
    11941 tgaaggtctg cttattctgt gttgaaactc ttcctgacgt atatgaagct gcttcttttg
    12001 ttgagtcggt atcaccatca gtaacttttt tgaccggatt tattattaat tttccatctt
    12061 ttacctgaat gttttcagcc gagtctacat actcctgcag ctcattattt acccatcctt
    12121 tttcatgtgt ctccacattc cagtcagcac ggtttaatga atcaccatta aactcatcgc
    12181 tccacacttt tttgtagcca tcatagctta gtttgcctgt ctgctgcgcc gcatccaacg
    12241 ctccctctgc aaacgtctgt gacggcatgg ctgataatac tctttttttc ataaagtacc
    12301 cctttcattt atagttattt gctgtctcgc atcatgttac tgtccacacc tttcagcgca
    12361 acccgtaacc atagcattac tataacgatt aagtatcctt aaaatataca aaaaaaagca
    12421 caaaaacatc aaattcatga cattttaatt ttcctgtaat ttccgcaatt ccctcggtgt
    12481 catgccaaac tgcgccttaa acatccggct gaacacctta taattcgtaa atccattgtg
    12541 ctccagtatg tccgtgatgg aatcctcagt ggccagaagc tcctcatgta tgtgattgat
    12601 tcttacctga ttgacatact cgagaaatgt aactcccatg cattttttga acatacggca
    12661 gaaataatcc tgattgagtg aggctatgtc tgccgcctcc tttaatgcta ttggctcctg
    12721 atagtttttc gccacatatc taaggacatc cttaaggcgc gctatctgtc tggattcttt
    12781 tcccgcattc atctctttgt acgaataatt tgtaaacaaa atatataaga attcgttcac
    12841 ttttgccata agccgaagct catagccctt gccctttttt cggtatatat gcgctatcga
    12901 cttaagaaga gacttcatct gctcatattt tctgctgcca tgctcggtct cataacactc
    12961 ccggaacctt atatgtccgt aatcgtctat gtagctgtcc agatatacat acggtatctg
    13021 caccagaagc accttgactc tttcatagct ttgcgtgcca tgtatcttat ttgagtttat
    13081 gacaaaaaat tcattttctt gcaatgtata ttcattttca ccgcagacaa cctgcagtga
    13141 gccggaaact acatagatta tttctatatg gtcatgccag tgcctcggtg aaaactgcct
    13201 gtagtcctca tatctgtcgc agtagattgg aagatgtggt attgatctga cgttttcgtg
    13261 tgtgtattgc tgcataattt tctccctgac ttactaaatc aaacttcgta tttaacgcta
    13321 tgataccttt tgacccttta atcatgctat attagcctga gcatatccaa ttattccgaa
    13381 gaaaacaata ttcatagttt gaaattctac cttcaaaata tactcctctc gcgcaaaaag
    13441 tcaatatcga cctaaaataa tgtcagtatc acaattgtat atatcgcttt tataatttac
    13501 aatcatctta tacgaaaaga acaagctccc gacatattaa tgtatgggaa gctctttaaa
    13561 taataaaaat ataagaaagg taatctgcta tgaacaaatg gtcacgattc aaattcactc
    13621 ctaatcttcc acttggggca aatggtgaaa gagttacagg aagcaaggct cacatagagc
    13681 tttcaaagga agctgccaag gaaggcatgg tacttctcaa aaatgaaaac aacgtactcc
    13741 ctcttgcagc gggttctaag gtagccctgt ttggaaaggg tactttcgac tatgtaaagg
    13801 gcggtggagg aagcggtgat gtaaccgttg catatatccg caatctctac gaaggactta
    13861 agcttcaaaa agagaaaata agcatatttg aggagctgtg cgattactac cgcaacgaca
    13921 ttaaaaagca gtatgcagcc ggtgctgtac ccggcatgac tatagagccg gatgtaccgg
    13981 ctgagctttt aagcaaggca caggcatata ccgacactgc tatcatctca atctgccgct
    14041 actccggaga aggctgggac agaaagagtg ttattgaccc aaacaacaag gctctctggg
    14101 actacgaacg cgagatgacc gagaagtcgg cagagctttt taaggatggt gatttctgtc
    14161 tctccgtaaa agaaaaggag atgattgaca ctgtaaaagc cagtttcaaa aatgtaatcg
    14221 ttattttaaa tgtcggtgga atggttgata catcttggtt tgcttatgat aatcagattc
    14281 aatcagcact gcttgcctta cagggcggca tggaaggcgg gctcgctgcc gctgagcttc
    14341 tcgtaggaga tggcaatcct tccggaaaaa ctgtcgatac atttgccaaa tcactcgacg
    14401 actatccgtc tacttacaat ttccacgagt caagagacta tgtggactat accgatgata
    14461 tatatgtcgg ataccgctat ttcgagacaa ttccgggagc tgccgagaag gttgtatacc
    14521 catttggcta tggactctca tacaccacat ttgatgtaga gaccgtttct gccggcgttg
    14581 tgaattccaa ctgtacgtct gaggccaata agctttatgc aaaagtccgt gtgacaaata
    14641 caggtaaatt ctccggaaaa gaggttgttc aggtatacat cgctaaacca caaggaaagc
    14701 ttggcaagcc tgcaaaggag ctcgcagcct ttgaaaagac aagagaactg cagccgggtg
    14761 aatcacagct tatgatcctc acatgggaaa taaacgatat ggcatcatat gatgaccttg
    14821 gaaa
//
LOCUS       DPUF01000029            6721 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23694, whole
            genome shotgun sequence.
ACCESSION   DPUF01000029 DPUF01000000
VERSION     DPUF01000029.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23694"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..75
                     /locus_tag="DHV81_01000"
     CDS             <1..75
                     /locus_tag="DHV81_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI93122.1"
                     /translation="AAEVAGISNDELKGMLEALLEVDK"
     gene            100..1014
                     /locus_tag="DHV81_01005"
     CDS             100..1014
                     /locus_tag="DHV81_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93123.1"
                     /translation="MKMTTKNAIEAAKITKKLKNFTLDVENFAIPQGFATALIGENGA
                     GKTTLLNILAGIRLDYKGEITYFGQYSDKQKENSGDIKNRIGYTGPGKYYLPQWTVKD
                     IEEISKLMFDDFSAEDFHRYCEELAIFSHGSIDMKKANSSFSDGMKMKLMLAGVMARK
                     TDLLLLDEPASPLDPLMRDKLCQMIGEYIHEGNGKRSVFFSTHNISDMENITDYAIIM
                     ENGQIVEQGFVEDLKEKYILIKGDAADTEAAGKVLYSMTKNPYGFEGICLAENIDKLA
                     GLNVTKEIPTLYQISVAVMKNNTKIVMR"
     gene            1031..1723
                     /locus_tag="DHV81_01010"
     CDS             1031..1723
                     /locus_tag="DHV81_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93124.1"
                     /translation="MKNMSGSMNEKNVSQKHVALTYIRDYRVMTNLKYRLITNILIPV
                     LAILASMLIACSKTVWAWIGVIVIVYDFYMMCEVVQDYFCFGCMCKKNAFGMHFLKTG
                     FNGVRYFENAVLVDILIRPLRIGITILIASIPFIKFGVNIWSIFDIIMISSIVSVGSL
                     NIFRYMDMGMYIYLLALIVILPAIAGSIAVLIYGGGMWFIAPVLAVVLVGVIAATYYH
                     MMVRIRRSYVDV"
     gene            1766..2428
                     /locus_tag="DHV81_01015"
     CDS             1766..2428
                     /locus_tag="DHV81_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93125.1"
                     /translation="MLKDIKLGFKLLRYGYKLKLNVIMFVFFAVIGIASDIMSKGTSI
                     IGGVYFMMCGMFTFQLIMSMDVSELIQSTSLKKKLQIYIPVMSSTVINLVVFTFLVIE
                     RVILIQNSVADKKQLIFTLFTLDVELLTVYLYTSICYKYYVLGFIVFMVLFMGVFTVF
                     SGAAFVPVSNAVFKLGLPVVALLGYIAIFAGGALEILIGELLYKKPLSEFAFRGFFRD
                     AK"
     gene            2602..3126
                     /locus_tag="DHV81_01020"
     CDS             2602..3126
                     /locus_tag="DHV81_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCI93126.1"
                     /translation="MDSDSYSKQQLDDLFMDMIAYYDGDPKRIQHFTKVHSYARLIGI
                     GEELDDASLFILEAAAYTHDIGIRVAEEKYGRCDGKLQEQEGPIIAQKMLSQLGFENY
                     IVERICFLIGHHHTYDNIDGLDYQILVEADFLVNLYEDDAGNRAIDKAYKRIFKTETG
                     KKIFRLMFGYEEED"
     gene            3289..3933
                     /locus_tag="DHV81_01025"
     CDS             3289..3933
                     /locus_tag="DHV81_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93127.1"
                     /translation="MANKINYQKLMEDLIKENCVDNNCTPRLLLHSCCGPCSTYCIQT
                     LSEYFKVTVFYYNPNIYPPEEYHMRAQEQKRFIDEFPVKNPVHFVEGVYDTRRFYDMA
                     RGMEAVPEGGERCFKCYRLRLEESAQYAREHGFDFFTTTLSISPLKNAEKLNEIGKDL
                     ESQYGVRYLFSDFKKKEGYKKSTEISKQYDMYRQYYCGCVFSKEQRDREIAARG"
     gene            4084..5483
                     /gene="argH"
                     /locus_tag="DHV81_01030"
                     /pseudo
     CDS             4084..5483
                     /gene="argH"
                     /locus_tag="DHV81_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569478.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     assembly_gap    4455..4523
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5687..>6721
                     /gene="asd"
                     /locus_tag="DHV81_01035"
     CDS             5687..>6721
                     /gene="asd"
                     /locus_tag="DHV81_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCI93128.1"
                     /translation="MSEKLKVGILGGTGMVGQRFISLLENHPWFEVTTIAASPRSAGK
                     TYEDAVGDRWKMDTPMPEAVKKIIVKNVNEVEEVASQVDFVFSAVDMSKDEIKKIEED
                     YAKTETPVVSNNSAHRWTPDVPMVVPEINPQHMEVIKYQKERLGTKRGFVAVKPNCSI
                     QSYAPVLTAWKEFEPYEVVATTYQAISGAGKTFKDWPEMVGNIIPFIGGEEEKSEKEP
                     LRLWGYVDDEKKQIVPAESPVITCQCIRVPVLNGHTAAVFVKFKKNPTKEQLIEALEK
                     FSGVPQELGLPSAPKQFIRYCEEDNRPQVALDVDYEHGMGINVGRLREDTVYDWKVVG
                     LSHNTVRGAAG"
BASE COUNT         2102 a         1123 c         1620 g         1807 t
ORIGIN      
        1 gcggctgagg tggccggaat ctcgaacgac gagcttaagg gaatgcttga ggcattgctt
       61 gaggtggata aataaagata tgaaaatgaa taaggagaaa tgaagatgac aacaaagaat
      121 gcaattgagg cagcaaaaat cacaaagaaa cttaaaaatt ttacactgga tgtggagaat
      181 ttcgcaatac cacagggctt tgccacagcc ttaatcggag aaaatggagc aggaaagaca
      241 acacttttaa atatattagc aggaatcagg cttgactata aaggtgaaat cacttatttc
      301 ggacagtatt ctgataaaca gaaggaaaac tcgggagata ttaaaaaccg cataggctac
      361 acaggaccgg gcaaatatta tctgccacag tggacagtta aggatattga ggaaatcagc
      421 aagctgatgt ttgatgattt ttccgcagaa gattttcaca gatattgtga ggagcttgca
      481 atattttcac atggcagcat tgatatgaag aaagccaaca gctcattttc ggatggtatg
      541 aagatgaagc tcatgctggc aggagtcatg gcgcgaaaga cagaccttct tttgctggat
      601 gagccggcat cgccgcttga tccgctgatg agagataagc tgtgccagat gattggcgag
      661 tatatccatg agggcaacgg taagcgcagt gtatttttct ctacgcacaa tatctcagat
      721 atggaaaata tcaccgatta tgccattata atggaaaacg gacagattgt ggagcagggc
      781 tttgttgagg atttgaagga aaaatatatc ctgattaaag gagatgcagc agatacagag
      841 gctgccggta aggtgcttta ttcaatgacc aaaaacccgt atggcttcga gggcatatgc
      901 ttggcggaaa atatagataa gcttgcagga cttaatgtga caaaggagat tcctacactt
      961 taccagataa gcgttgctgt catgaaaaat aacacaaaga ttgtgatgcg ctagtgcgag
     1021 gtgaggtgtt atgaaaaata tgtcaggaag tatgaatgaa aaaaacgttt cacaaaaaca
     1081 tgtagctcta acatacatca gggattaccg tgttatgact aatctcaagt atcgtctgat
     1141 aacaaatata ctgattccgg ttttagcaat tctggcgtca atgctaattg cttgttcaaa
     1201 aacagtctgg gcatggatag gagttatagt aattgtttat gatttttata tgatgtgcga
     1261 ggtggtgcag gattatttct gcttcggatg tatgtgcaag aagaatgcat ttggcatgca
     1321 tttcctaaag acaggtttta acggagtaag atactttgag aatgcagttc ttgtggatat
     1381 acttataaga ccgctgcgta ttggaattac aatactcatt gcttcaatac catttattaa
     1441 atttggagtt aatatctggt cgatatttga cattattatg atatcttcaa tagtgtcggt
     1501 tggctcgtta aatatattca gatacatgga tatgggaatg tatatttatc tgctggcgct
     1561 tatagttatc ttaccggcaa ttgcaggtag tatagctgtt ttgatttatg gcgggggaat
     1621 gtggtttatc gcacctgtgt tagcagtggt gcttgtagga gtgatagcag cgacatatta
     1681 tcatatgatg gtgcgtatcc gcagatcata tgttgatgtg tagaggcatt ttaaattgga
     1741 agtatatttg aaaaggagaa atattatgct gaaagatatt aaattaggat ttaaattatt
     1801 gagatatggc tacaaactga agttgaatgt gatcatgttt gtattctttg cagtaatcgg
     1861 aattgcatcc gatataatga gcaagggaac ttccatcata ggtggtgtat actttatgat
     1921 gtgcgggatg ttcacatttc agctcatcat gtcgatggat gtgtcagagc ttatccagtc
     1981 cacatcactg aaaaagaagc tgcagatata cattccggtg atgtcaagca cggtaatcaa
     2041 tcttgttgtt ttcacatttt tggtaataga aagagtcatt ctgatacaga attccgtagc
     2101 agataagaaa cagcttatct tcacactttt tacgctcgat gttgagctgt tgacagtata
     2161 tctgtacaca agcatatgct ataagtatta tgtacttggc tttatcgtat tcatggtttt
     2221 gtttatgggc gtttttactg tgttttcagg agcagcgttt gtgccggttt ctaatgctgt
     2281 ttttaagctt ggactcccgg tggtggcatt gctaggctat attgctattt ttgcaggtgg
     2341 agcgcttgag atattgatag gagagctgct ttacaagaag cctttgtcag aattcgcatt
     2401 caggggattt ttccgtgatg caaagtagtg atgttactgt tcccacataa cagtgtgaca
     2461 aacaacatag tgatttcaaa aaataagaaa tatttgtgta aaaatattga aaaattgatc
     2521 gctttgtagt atgttatcat tgtcggtgca aaagccggca atgagactaa ttaacatatt
     2581 atcaggagaa aaatatatta aatggatagt gacagttata gtaaacaaca gttagacgac
     2641 ttgtttatgg atatgattgc ctactatgat ggcgatccaa agagaatcca gcatttcacc
     2701 aaggtgcaca gctatgcaag gcttatcggt ataggagagg agcttgatga tgcctcgctt
     2761 tttatacttg aggcagcggc ttacacacac gatatcggca tacgtgtagc ggaggagaag
     2821 tatggcaggt gcgatggaaa gcttcaggag caggagggac cgataatcgc ccagaagatg
     2881 ctctcacagc ttggctttga gaattacata gttgaaagaa tatgttttct cataggtcac
     2941 catcacacat atgacaatat agatggtctt gattatcaga tactcgttga ggctgatttc
     3001 ctcgtaaatc tgtatgagga tgatgcggga aaccgtgcaa tcgacaaggc atacaagcgc
     3061 atattcaaga ctgaaacagg aaaaaagatt tttagactaa tgttcggata tgaagaagag
     3121 gattagtatg agccgggccg ggaggagact tccggccctt gttcgtatta tgcggcttat
     3181 atcagaaata gtaattaatc agaatagtaa atatgcagaa tagttacatg aaataatatt
     3241 tggggatatg tcaaaaattt ttgaattttg agaaagaaat aatataatat ggcaaacaaa
     3301 ataaattatc aaaaattaat ggaagattta ataaaagaaa attgtgtgga caataactgc
     3361 actccacgtc ttttgcttca tagctgctgt gggccgtgca gcacatactg catacagacg
     3421 ctctcggagt attttaaggt gacggtattt tattacaatc caaacattta tccgccagag
     3481 gaatatcaca tgagagcaca ggaacagaag cgctttatag atgagtttcc ggtgaaaaat
     3541 ccggttcact ttgtggaggg tgtgtacgac accaggcgct tttacgatat ggcacgcggc
     3601 atggaggctg tgcctgaggg cggggagagg tgctttaagt gctacaggct gcgtcttgag
     3661 gagagtgcac agtatgcaag ggagcatggc tttgattttt ttactacgac actttccatc
     3721 agcccgttga agaatgccga aaagctcaat gagataggaa aagacttaga aagccaatat
     3781 ggagtgcgat atcttttctc tgattttaag aagaaagagg gctataaaaa gtccacggaa
     3841 atatcaaagc agtatgatat gtacaggcag tactactgcg gctgtgtatt ttccaaggaa
     3901 cagcgtgaca gagagattgc agcacgtggg tgaaaatgtg gttgcgtgac agcaaaaatt
     3961 attatataat ttagcacagt aaagcggctg tttagagcag acggcggtat cacatcttat
     4021 ttttatataa tacattgtga tacatgaaaa taaatcatca tttaataatc aaggaggaat
     4081 ctcatggcac agttatgggg aggacgtttc acaaaggaga cagatcagct cgtttacaat
     4141 ttcaatgcgt ccatttcatt tgatcagaag ttttataaag aggatataga gggcagcatc
     4201 gcccatgtca cgatgcttgg aaagcagggc atcatctcac aggcagagag caacgatata
     4261 gtggcttgcc taaagcagat acttaaggag gtagagtcag gggagcttga aatcagctca
     4321 aagtatgagg atatccacag tttcgttgag gccacactca tagacaggct tggtgacaca
     4381 ggaaagaagc ttcacacagg ccgaagcaga aacgaccagg tagctcttga catgcgtctt
     4441 ttcaccagaa agacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnaacacac agactatcat gccgggcttt acacatctgc
     4561 agaaggccca gccaatcact ctggctcacc atatgggcgc atattttgag atgtttaaga
     4621 gagacagatc aagactgtgc gatatatata agagaatgaa ctattgtccg cttggctcag
     4681 gagcactcgc aggcactaca tatccgcttg atagggacta tacagcacag cttctggatt
     4741 tttacggacc gacacttaac agtatggatg gtgtgtcaga cagagattat ctgattgagt
     4801 ttttatcggc tctttcaact atcatgatgc atctgtcacg tttttctgag gaaattatca
     4861 tatggaattc aaacgagtac cagtttgttg agattgacga tgcgtacagc acaggaagct
     4921 caatcatgcc acagaagaaa aatcctgata tagcagagct tgtcagagga aagacaggcc
     4981 gtgtatacgg tgcgctcatg tcgcttctca ccaccatgaa gggtattccg cttgcgtaca
     5041 acaaggatat gcaggaggat aaggagctgg catttgatgc attcgacact gcaaagggat
     5101 gtatcgcact ttttacagga atgattgata caatgaagtt taacaaggac gtgatgagaa
     5161 agagtgcaaa caatggcttt acaaatgcca cagatgcagc tgattatctc gttaaaaagg
     5221 gggtaccatt tagagatgca catggtattg taggaagaat agtgctttac tgcatcgaca
     5281 agggcattgc aatcgacgat atgagcatcg acgagctcaa ggctatctct ccggtatttg
     5341 agaaggatgt atttgatgcc atttcaatgg agacctgtgt cagcacaagg tgtacagttg
     5401 gagcaccatc aaagacatca atggacaagg taattgccgt ttacgacgag tatatgaagt
     5461 caaattggca ggatgaagtg tagtttttgt gcatatttta taaaaaacat ggagaaaaat
     5521 tattaattta atatagtttt ttccttgttt ttcattagag tttcaagtaa tatatttcct
     5581 ataaagacag ttgtacgtga cggacggaca aaatgaaaat agtcgatatt aaaaataaac
     5641 gtatgacaag gtcgaagaaa atctagaaga tagggagatt agagtaatga gtgaaaagct
     5701 taaagtaggt atcctcggtg gaacaggaat ggtaggacag agatttattt ctcttttaga
     5761 gaaccatcca tggtttgagg taacaacaat tgcagccagc ccaagaagtg caggcaagac
     5821 atacgaggac gccgttggtg atagatggaa aatggacaca ccaatgcctg aggcagttaa
     5881 gaaaatcatc gtaaagaacg tcaatgaggt agaagaggtt gcttcacagg ttgacttcgt
     5941 attctcagcc gttgatatgt caaaggacga gatcaagaag atcgaggagg actacgcaaa
     6001 gacagagact cctgtagttt caaacaatag tgcccacaga tggacacctg atgtgccaat
     6061 ggttgtacct gaaatcaacc cacagcacat ggaggttatc aaatatcaga aggagcgtct
     6121 cggtacaaag agaggcttcg tagcagtaaa gccaaactgc tcaatccagt catatgctcc
     6181 ggttcttaca gcatggaaag agtttgagcc atatgaggtt gttgctacaa catatcaggc
     6241 aatctcaggt gccggaaaga cattcaagga ctggcctgag atggttggaa acatcattcc
     6301 attcatcggt ggagaggaag agaaatctga gaaagagcca cttcgtctct ggggatatgt
     6361 ggatgatgag aagaaacaga ttgttcctgc agagtcaccg gttatcacat gccagtgcat
     6421 ccgtgttcct gtattaaacg gtcatacagc agctgtattt gtcaagttca aaaagaaccc
     6481 tacaaaggag cagctcatcg aggcactcga gaagttcagc ggagtaccac aggagcttgg
     6541 gcttccaagc gcaccaaagc agttcatcag atactgtgaa gaggataatc gtcctcaggt
     6601 tgcacttgat gtggattatg agcatggtat gggaatcaat gttggacgtc tcagagagga
     6661 tacagtctat gactggaagg tcgtcggact ctcacacaac acagtacgtg gtgcagcagg
     6721 g
//
LOCUS       DPUF01000030            4735 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_22139, whole
            genome shotgun sequence.
ACCESSION   DPUF01000030 DPUF01000000
VERSION     DPUF01000030.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_22139"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(43..942)
                     /locus_tag="DHV81_01040"
     CDS             complement(43..942)
                     /locus_tag="DHV81_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI93129.1"
                     /translation="MTLQQLKYVVTVAECGNITEAAKQLFVSQPTVTSAIHDLEKEMQ
                     IVIFIRTNKGVILSEDGERFLGHARQVLEQVDLIEGIYKDDVVSKPHFAVSCQHYSFA
                     VNAFVDVIREFDAYQYDFTIREEQTHEIIEDVTNLKSELGVIYLSSKNTEVLSKMLKR
                     NELEFTELFTATPHVFICDSHPLASKKSITMDDLQDYPYLTYEQGEYNSQYFSEEFVS
                     TLDSRKNIRVRDRATLFNLIIGLNGYTVCSGVISAELNGRNIISKPLDVEEYMRIGII
                     SRKGVVLSRYAREYIEALKAHCM"
     gene            complement(1023..1931)
                     /locus_tag="DHV81_01045"
     CDS             complement(1023..1931)
                     /locus_tag="DHV81_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCI93130.1"
                     /translation="MKSTAFDMAMMAYRQNDMEGAFRQFLPLAETGDADAMNMAANMF
                     ERGQGTYRDPGRAVFWWKKAVELGNVDALADYGQYLVTTFFDGKEQKLGVGYLVTATE
                     RGNNRAGESLVEFALKNNDAGVKVYRTAADYCDRAIDAATDSYLRTQYVQKRGMLKYK
                     LRQHRLGNNYVYLAAVFSAIGAVFLMIASVDLFLELHMEYCDMFKLFSYSKMIPWEAD
                     IAMFFGAMLLFTLGKVTNKLHVASFLQLLASVFSVAVVAMHFICVINDGRVWYDKLLW
                     YVVLVVIPLMLGKLLGEILRKIIGIQ"
     gene            complement(2019..3185)
                     /locus_tag="DHV81_01050"
     CDS             complement(2019..3185)
                     /locus_tag="DHV81_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCI93131.1"
                     /translation="MCRKREKIVKKICIAIISIALIIAFGISLNNVEKTELQSTDGRD
                     FEKATVTEIVSSDYSDSTSKDYDGSVGEDDSVRAGQQQIKVKIRTGEHKGEILDATSS
                     SSYLYGARCRIGTKVIVIISESDELTTVSVYNYDRGNQLYMIIAFFLIVLVLIGGFKG
                     FKSAVGLVFTFCCILFVFMPLIYRGVSPIFAAAFVVIITTVVVMYLIDGFTAKSICAI
                     VGTIIGVVLAAVFAFIFGKICHISGYNVDDIESLIYIGEMTDIKVGELMFAGILISAL
                     GAVMDVAMSVASTINEIHDKNPKLDTKELFKSGINVGKDMMGTMSNTLILAFAGGSIN
                     TFVYIYSYNYQYYQVLDMYSIGIEIIQGISATLGVILTVPIVSAISAVALKKNI"
     gene            complement(3161..4717)
                     /locus_tag="DHV81_01055"
                     /pseudo
     CDS             complement(3161..4717)
                     /locus_tag="DHV81_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase"
BASE COUNT         1281 a         1159 c          788 g         1507 t
ORIGIN      
        1 tttatgatta atcagattac tactttctcg gtgtagtgtg ggtcacatac agtgcgcctt
       61 caatgcctcg atatactccc ttgcatatcg cgagagtacc acaccctttc tgctgataat
      121 gcctatcctc atatactcct ccacatcaag tggctttgat ataatattcc ttccattcag
      181 ctcagcggaa atcactcctg agcacactgt gtagccattt aagccaatta tcagattaaa
      241 aagtgtagct ctgtctctta ctctgatatt ctttctgctg tcaagagtgc tgacaaactc
      301 ctccgaaaag tactgtgaat tgtactctcc ctgctcatac gtcaggtacg gatagtcctg
      361 cagatcatcc atcgtgatgc tcttttttga agcaagcggg tgactgtcac atatgaatac
      421 atgcggtgtg gctgtaaaaa gctctgtgaa ctcgagttca ttacgcttta gcatctttga
      481 aagcacctcc gtattcttgg agcttagata tatgactccc agctcactct ttaaattcgt
      541 gacatcctca ataatttcgt gagtctgctc ctccctgatg gtaaaatcat actgatatgc
      601 atcaaactct ctgattacat ccacaaatgc attcactgca aaggaatagt gctgacacga
      661 taccgcaaag tgtggcttac tcaccacatc atccttgtag atcccctcta tgagatctac
      721 ctgctcgagc acctgtctgg catgaccgag aaatctctca ccatcttctg atagtatcac
      781 tcccttgttt gtcctgatga aaataactat ctgcatctct ttttccaggt catgtatagc
      841 gctggtcaca gtgggctgcg agacaaaaag ctgctttgca gcctctgtta tattgccgca
      901 ttctgccact gtcaccacgt actttaattg ttgcagagtc atgttcattc tccttttgtt
      961 acatatcgaa atattatttt acatattaaa tgtcttacat cgcttaagtc atctcgaaac
     1021 tattactgta taccaataat ctttctcagt atctcaccca acagctttcc gagcataagc
     1081 ggaatgacaa caagcaccac ataccacagc agcttgtcat accacaccct gccatcattt
     1141 atcacacaga taaagtgcat ggcaacaact gccactgaaa atacagatgc aagaagctgc
     1201 aaaaagctcg ccacatgaag cttatttgtt accttgccaa gcgtaaataa aagcatcgca
     1261 ccaaagaaca tagctatatc agcctcccat ggtatcattt tactatacga aaacagctta
     1321 aacatatcgc aatactccat atgcagctca agaaacaaat ccactgatgc aatcatgaga
     1381 aatactgctc ctatcgctga aaataccgct gccagatata cataattgtt tccaagtctg
     1441 tgctgtctga gcttatattt aagcataccc cttttctgca catactgtgt gcgaagatat
     1501 gaatctgtcg cagcgtcaat agcccggtcg cagtaatctg ctgctgtcct gtatactttg
     1561 actccggcat cgttattttt aagggcaaac tccacaaggc tctctcctgc cctgttgttg
     1621 ccacgctccg tcgcagtaac cagataccct acaccaagct tctgctcctt gccatcaaaa
     1681 aaggtagtga ccagatactg accataatcg gcaagtgcat ccacatttcc aagctccact
     1741 gctttcttcc accaaaacac tgcgcgcccg ggatcccggt atgtgccctg gcctctttca
     1801 aacatatttg ctgccatatt catggcatct gcatcgccag tctctgccag tggcagaaat
     1861 tgacggaatg ctccctccat atcattttgt ctgtatgcca tcatagccat atcaaacgct
     1921 gtacttttca tattgttctc cttgacagcc ttagctgttt tcttcttatt ataatgtatt
     1981 taacatgatg atagcaatac gacttctttc ccaatttatt atatgttttt ctttagtgct
     2041 acagcagata ttgcagacac tataggcacc gtgagtatca ctccaagcgt tgcagaaatt
     2101 ccctgaatta tttcaattcc gattgaatac atatcaagca cctgataata ctgatagtta
     2161 tagctgtaaa tatatacaaa ggtattaatc gaaccacctg caaaagccag aatcagcgtg
     2221 ttggacatgg ttcccatcat gtcctttccg acatttatgc cggatttaaa cagttctttg
     2281 gtgtcaagct ttggattttt atcatgaatc tcattaatag tgcttgctac agacatggca
     2341 acatccatca cagcaccaag agctgatatc agtatccctg caaacatcag ctctcccacc
     2401 tttatatctg tcatctcacc aatataaata agactttcga tatcatccac attatagccg
     2461 gatatatggc atatttttcc aaatataaat gcaaaaacag cagccaacac cacacctatt
     2521 attgtgccga cgattgcaca gattgatttt gcagtaaatc catctatcag atacataaca
     2581 acgactgttg ttataattac cacaaatgcc gccgcaaaaa taggagacac tcctctgtag
     2641 ataagcggca taaatacaaa taaaatgcaa cagaatgtaa acacaagtcc tacagctgat
     2701 ttaaagccct tgaatcctcc aattaacact aaaactataa ggaaaaaggc aattatcata
     2761 tacagttgat ttcctctgtc ataattatag acacttaccg ttgtaagctc atccgactcg
     2821 ctgattatca ctattacctt tgttcctatt ctacatctgg caccatacag atacgatgaa
     2881 gaactggtgg catccagaat ttctccctta tgctcaccag ttcttatttt gacctttatc
     2941 tgctgctgcc cagccctgac actgtcatct tcacccacag aaccatcata atcctttgaa
     3001 gtgctatccg aataatcaga gctgacaatc tctgtcaccg tcgctttttc aaaatcacgg
     3061 ccatcagttg attgcagttc tgtcttttcc acattgttca gtgatattcc aaatgcaatt
     3121 atcagagcaa tacttattat tgcaatacag attttcttca ctattttctc tcttttccgg
     3181 caaataccac tgctgttcct gctgctccaa gaccaatcac acaaacaatt gtccaaagca
     3241 gtgcctgtga ctcatcaccg gtcttaacct tagaaactga tttattgttt gacttttctg
     3301 atgctttctc tgattcagat gttttttctg ttggctgctc tgatgctttc tctgattcag
     3361 atgtcttttc tgttggctgc tctgatgctt ttggtggttc ctgtgcagtc acctttacaa
     3421 gaccgtttct ggctgttgtc aggttctcaa gtatcttgtt gatctgtgcc tgctcagtct
     3481 cagggctgat acggtttgcg gcatccatcg catcctttaa agccttccag ctaccagttg
     3541 tgtagtcctt ctctacaagt ttattatctg taagctcttt atttatctgc gcaagcttag
     3601 catcaactgc tgatttgtct gctgatttta caatagtaac cgtattgtca attgcactgt
     3661 cattgtcagt acgataagtg ttaattgtca tggttgtctc tgtcacatcg atgagtgaat
     3721 atgtagggac atcctcctgc catctgcttg ctatgtatga ctgctgacgt gatacgaggt
     3781 cataatactt acttcctgat gaactgctgg ctgtaagata aagaattccc ttaggatcta
     3841 ccacatactc tcctgtctct gtaattctgt ctgcatccat cacagaagta agataagata
     3901 agtatgtctt ttctgattcg tctgttgtgt ctgctttgat atttcccggt gctacagtca
     3961 aagtaccctc acctgtatag tcaatatcct tatcaagcat ttcatcaaac tcatcatcag
     4021 tatatgttac agtcttttct atctgattgc ctgaaagaaa tttacttctt gaataagcat
     4081 ggtcatgtcc tgtaagcaca gcatctacac catatttctc aaatgtcggt gtaagcgcat
     4141 aacgtaaatt cacaatctcc ggctcattcg aatgctctgc cgaaccataa atatcctgat
     4201 gcaatgtaac taccttccac ttgcaatcag cattttctgc aactgtttct ttgataaact
     4261 gaatatgctc tgctgagttt gtgtcctgtg tattaagcat cataaaaagc acatcaccat
     4321 atgtgaaata atagtcacca ccaacttttt tattatctcc cagattgctg aggtttggaa
     4381 cattaaaatg gtactgatag ttggcattgt ctgcatcatg attaccgact gttgttgcta
     4441 ccggcaatga cgcaagtgta tccggtgaaa gatatcctgc atactctatc tcacttgttg
     4501 tagaactctt tcccggagct tttttctttg ttgtctgaat ctggtctcct gctgacacaa
     4561 taaaatcagt tcctgcactc tgtatctttt tcaatgtctg tgaccaattg tagctgtcat
     4621 ttgctaccgc attgctctgt gcattgtaaa acgcctctgt atcagctccc tttaattcat
     4681 tagatgaacc aatctgtgga tcacctacaa aagcaaattc tttaggttac aatcg
//
LOCUS       DPUF01000031            8140 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_34492, whole
            genome shotgun sequence.
ACCESSION   DPUF01000031 DPUF01000000
VERSION     DPUF01000031.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_34492"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="DHV81_01060"
     CDS             complement(<1..864)
                     /locus_tag="DHV81_01060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93132.1"
                     /translation="MFGNRAKISAEELAYMKEQLDNDRGFFENVNAKSKLIEADFDEL
                     EKSRQLEESSLKQLNDNANNVVEFSKDSMDAISALNDSFTECVKAAADNLTSLEGAAK
                     AIATQHEDTCALVENNKHFTAPAKSLSETSDRLEEQVDSCADIAAQMKEQNKQMSVLS
                     LNAAIEAGMLGEQGKLFVEAAESIREASVSYDSAIDAVEQELSEAKAEISALKEQVSH
                     LVGLLKDNNVATTKLMKQGVELNHVFSQCDEISVDMIEACRQQIVSIRNTQEEIIKFE
                     ERNKLQIEDAYA"
     gene            complement(907..2642)
                     /gene="recJ"
                     /locus_tag="DHV81_01065"
                     /pseudo
     CDS             complement(907..2642)
                     /gene="recJ"
                     /locus_tag="DHV81_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742171.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
     assembly_gap    1573..1665
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2623..3387)
                     /locus_tag="DHV81_01070"
     CDS             complement(2623..3387)
                     /locus_tag="DHV81_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCI93133.1"
                     /translation="MNTETQNYKIIVAYDGTRYKGWQVQKSTDDTIQGKLQHVLSTLA
                     GKPVEVIGSGRTDAGVHAVGQVASFHMPKHFSKDEIFIWLNEHLPADIAVTDISNVPD
                     RFHARYNAVSKTYVYTIHTGIVSDVFRRKYVYDYDKPLDTDRMKKAAAYLLGEHDFKA
                     FCGNRHMKKSTVRTIFSIDIEKTGADIVISYTGDGFLQNMIRIITGTLIEVGDGRKNP
                     DAVMEILASKDRAQAGYTAPACGLRLERVDYGKVVC"
     gene            complement(3499..4761)
                     /locus_tag="DHV81_01075"
     CDS             complement(3499..4761)
                     /locus_tag="DHV81_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HCI93134.1"
                     /translation="MKNLHILQQESIDEGRLQVRVRQKNMGTPVADARVSVSYSGDPQ
                     GKIEETDTNESGSIEAVEIATPPLEYSMSPSESQPYSEVTVTVSANGYRNITVSGVEV
                     MPDRLSIQDIELEVLDAPGNDVDNIVIPAHTLYGDYPAKIPEPEITPVAETGEIVLNR
                     VVIPEYVIVHDGAPSDSTAANYYVRYKDYIKNVASSEIYATWPDATIRANVLAIMSFT
                     LNRIYTEFYRGKGYNFNITSSTAYDHKFIYGRNIYDNISLIVNEMFENYLSRPNVKQP
                     ILTQYCDGQKVSCPSWMTQWGSKSLGDQGYSAIEILRYFYGSNMYINTAEAVSGIPAS
                     WPGYNIGIGSSGQNVYQIQKQLARIAKAYPAIPSIVPDGIYGPKTKAAVEKFQAVFGL
                     PVSGVVDYNTWYEISNIYVAVTRIAELA"
     gene            5294..5800
                     /locus_tag="DHV81_01080"
     CDS             5294..5800
                     /locus_tag="DHV81_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HCI93135.1"
                     /translation="MHTFQPYPIDMLDINPFTKLSKEWALVTAGDKEKSNTMTVSWGG
                     TGVLWGKNVVFIFIRESRYTKNFIDNGEFFSLSFLSEKYRDALKYCGAHSGRGEDKWS
                     KAGLSPATRHGIPYPDEANLVFLCRKMAAVPIEEASFTDKAIMHQWYGDHDMHTMYVG
                     EIIEVAAR"
     gene            complement(6164..6676)
                     /locus_tag="DHV81_01085"
     CDS             complement(6164..6676)
                     /locus_tag="DHV81_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCI93136.1"
                     /translation="MNNIEMKNRWIALYVENEVGVLAKVSGLFSAKSYNLQSLTVGTT
                     EDETISRMTICVTSDDITFEQIKKQLNRMVEVIKVIDLTDVPLHMKEILYVRVSKLTR
                     EEITDIFQTAQVFNIDVVDKNADSVILESKLTEHRNDDLIALLKSQYKHISVVRGGAV
                     AIEAMSTGCR"
     gene            complement(6689..>8140)
                     /gene="ilvB"
                     /locus_tag="DHV81_01090"
     CDS             complement(6689..>8140)
                     /gene="ilvB"
                     /locus_tag="DHV81_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCI93137.1"
                     /translation="DNVPLVCFTGQVPLPLIGNDAFQEVDIVGITRNITKYSVTVRDR
                     KDLGKIIKMAFHIARTGKPGPVLIDLPKDIQTASGPAEYPDKVEIRGYRVNDTVHVGQ
                     LKRAYKMLKSAKKPLILAGGGINVARANENLRRFAEAENVPVVTTIMGKGAIPTTHPL
                     YVGNCGMHGKYAANKAVSECDLLFSIGTRFNDRITGDLNEFAPKAKIVHIDVDTASIS
                     RNVVVDVPIVADANVALDKLNEWAEPKKTAEWQKQIKAWDEENPLGMRRDKGMTPQMI
                     MEHINKNYKDAIYVTDVGQHQMWATQYLELDSNSQLLTSGGLGTMGFGLPAAIGAKIA
                     NPDKDVVCISGDGGLQMNIQELATSVVQGAGVTICVLNNYYLGMVRQMQQLFYGKRYS
                     ATCLRRRKGCPDECKGPNEACPPYTPDFLKLVESYGGHGIRVDKEEDIDAAFAEAKKY
                     KDVPVIIEFMIATDEIVLPMVKSGNPMSEMILK"
BASE COUNT         2044 a         2032 c         1500 g         2471 t
ORIGIN      
        1 tgcgtatgca tcctctatct gcaacttatt gcgctcctca aacttgataa tctcctcctg
       61 tgtattgcgg attgacacaa tctgctgtct gcacgcttct atcatgtcaa ctgaaatctc
      121 atcacactgt gaaaacacat gattgagctc cacaccctgc ttcatgagct ttgtcgtggc
      181 aacattgttg tcctttagaa gccctacaag gtggctcacc tgctctttaa gtgcactaat
      241 ctcagctttt gcctctgaaa gctcctgttc aacagcatca attgcactgt catatgaaac
      301 agatgcctcc cttatgctct cagcagcctc tacgaacagc tttccctgct ctccaagcat
      361 gcccgcctct atagcagcat ttagtgagag aacactcatc tgcttattct gctccttcat
      421 ctgcgcagct atatccgcac agctatccac ttgctcctca agcctgtccg atgtctctga
      481 caagctctta gccggagctg taaaatgctt attgttttcg acaagcgcac atgtatcctc
      541 atgctgtgtg gcaattgcct ttgccgcacc ctcaaggctt gtaagattgt ccgcagccgc
      601 ctttacacac tctgtaaagc tgtcgtttaa ggctgatatg gcatccatgc tgtcctttga
      661 aaattccacc acattatttg cattatcatt tagctgctta agggatgact cctcaagctg
      721 tcttgatttc tccagctcat caaaatcagc ctctataagc tttgactttg catttacatt
      781 ctcgaaaaat cccctgtcat tatccaattg ttccttcata tacgctaatt cctcagcgga
      841 tatttttgct ctgttcccaa acactgatct attctccttt accggttacc tttttagaat
      901 ataatatcac ttataatact gtatttcaaa ctgcagtgac tcattttccc tgaatacatt
      961 tatctttggt gaatatacta cggacagcac tatattattg aaccttcctc tttgtgcagc
     1021 ttcaagcgcg tttgaaccaa acctttgcct gatatagtca aaaaagcttt ctatatcacc
     1081 aaaataaatt gcattgattg gcgtgccata tgaggatatc aaagaaagtc taagcacatt
     1141 gttattttta ccaactaccc acatgcggct cacacgtaga tttttgtctg caaaaacagg
     1201 ctttggattg tcctttccaa acggtgccag tactgaaaac tcagacacca gtctcattga
     1261 tacataatcc accggcatcg gcacatctat atgtatttta ggcacgaggt catcgtcagt
     1321 aagcgatgca tactcattta ttttctgacg gaatggctca acgttttctt gtggcaatga
     1381 aagtcctgca gccatcgggt gaccaccaaa cttcgtgaac agctcactgc atttgcacat
     1441 ttcctcatac atggagtact cctctatcga gcgccctgag ccctttactc cgtcctcact
     1501 ctttgtgagt ataaaggttg gcttattgta tctctccctg atgcggcccg ctatgattcc
     1561 tgctatgctc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctac aagctctgat
     1681 gcgatgcgca tggcatcctc ctgcctgtca cataaaaaca gctcaagtgc tcttttggct
     1741 gtatccagac ggcctgtcgc attgacacag ggtccaagaa caaatccaaa atgatatgca
     1801 tctacatttt cagcctctat attacaacat tcaagaaggg ctttcatgcc gatatttgtc
     1861 gtatgatgaa tactctttaa accggccttt acgagtatac ggttttcacc cgtcagcggc
     1921 ataacatcac cgactgttgc aaatgccaca ttctctataa aatccatggc ctctttctca
     1981 tctatgccga tttttctata tagtaatatg acaattttcc aggcaacagc tgctccgcaa
     2041 agctccttgt acggatagct gcactctatc tgatgcggat ttacaacggc atctgctttg
     2101 ctctccttgt aaatctttat accctttata tcttcaaaag gcactgcatg gtggtctgtt
     2161 acgagaactg tcataccaag ttcctttgca tgggcaattt cctcaatcgc cgcaattccg
     2221 ttgtcacatg tgaccacagt gtctatcccg tcctcatatg ccttgtcaat cagatgcaca
     2281 ttcaaaccat agccatcctt cactctgtcg ggaatcatat aatccacaca tgcaccggct
     2341 cttttaagtg ccttcacaag aatatatgta gacatcactc cgtctatgtc atagtcgccg
     2401 attatcctga ttttggcctt gtcagctatc ttttgggcaa gaatgtcaac cagcctctga
     2461 ccatccttta aaagagccgg gtctgcaatg tctgagattt tgccgtttaa aaattcattt
     2521 accgcgtcat ctcctaccac acctctgttt ctgattatcc gcgccgtcac ctgatctatt
     2581 ccaaactttg ctccgattgc cgcaaaatca gcctttttcg cactaacata ccactttgcc
     2641 ataatctacc ctttccagtc tcagcccaca cgccggtgct gtgtagcctg cctgtgccct
     2701 atcctttgaa gctaaaattt ccatcacagc atccggattt ttccttccat cgcccacctc
     2761 gataagcgtt cctgtgatga ttcttatcat attctgtaaa aatccatctc cggtgtagct
     2821 gataacaata tcagcgcctg ttttttctat atcaatagaa aaaatcgtcc tgaccgttga
     2881 ctttttcata tgtctattgc cacaaaaggc tttaaaatca tgctctccga gaagatacgc
     2941 tgctgctttt ttcatcctgt ctgtgtcaag cggcttatca tagtcgtata cgtattttct
     3001 cctgaacaca tcggacacaa taccggtgtg tattgtatac acatacgtct ttgacacagc
     3061 attgtacctg gcatgaaatc tgtcaggcac atttgaaata tctgtcaccg caatatctgc
     3121 cggcagatgc tcattcagcc atataaatat ctcatccttg gaaaaatgct tcggcatgtg
     3181 aaagcttgcc acctgcccca ccgcatgcac tccagcatcc gtgcgccctg agcctatgac
     3241 ctcaaccggc tttcctgcca gcgttgaaag cacatgctga agctttccct gtattgtatc
     3301 atctgtggat ttctgcacct gccagccttt gtagcgtgtg ccgtcgtatg ccacaattat
     3361 cttgtaattt tgggtttctg tattcataaa attgtctttc tttatctaaa aaaatagggt
     3421 tgtgaatagt tacttttatt ttatataata tcacatcatt ttttctcttg ccagcactgt
     3481 tttgctcctt taatccaatt aagcaagctc cgcaatacgt gtcacagcga catatatatt
     3541 tgatatctca taccatgtat tgtagtccac cacacctgat accggaagtc cgaatacagc
     3601 ctgaaatttt tccacggcag cctttgtctt tggtccgtat attccatccg gcacaataga
     3661 tggaatggcc ggatatgcct ttgcgatgcg ggcgagctgt ttttgtatct ggtatacatt
     3721 ctgtcctgac gagcctatgc ctatattgta gccgggccat gacgccggga ttcctgatac
     3781 agcctccgct gtattgatat acatgttgct gccgtaaaag tatctgagta tctcaattgc
     3841 actgtagccc tggtcaccga gacttttaga gccccactgt gtcatccagc tcggacagga
     3901 gaccttctgt ccgtcacagt actgcgtcag aatcggctgc ttcacattag gtctcgacag
     3961 atagttttca aacatttcat ttactatcag cgagatattg tcatatatat ttctgccata
     4021 gatgaatttg tggtcatatg ccgtagacga agtgatatta aaattatagc cctttccgcg
     4081 ataaaactcc gtgtatatcc tgtttaacgt aaatgacata attgcaagca catttgccct
     4141 tatagtggca tcaggccagg tcgcatatat ctcactggaa gctacatttt tgatgtaatc
     4201 cttatatctg acataataat ttgccgctgt ggaatccgat ggtgccccat catgcacaat
     4261 aacatattcc ggaatcacaa cacgattaag tacaatctca ccggtctcag caaccggcgt
     4321 aatctccggc tcaggtatct tggcaggata atcaccgtag agtgtatgtg ccggaataac
     4381 aatattgtcc acatcatttc ccggtgcatc aagtacttcc agctcaatat cctgtatgga
     4441 aagtctgtct ggcatcacct caacgcccga aacagtgata tttctgtatc cgttagctga
     4501 aacagttacc gtcacctccg aatacggctg tgactcactg ggcgacatag agtactcaag
     4561 tggcggagtg gcaatctcaa ctgcctctat gcttcccgat tcgttggtat cagtctcctc
     4621 tatctttccc tgcggatccc cggaatagct gactgacacc cttgcatccg caaccggcgt
     4681 ccccatgttt ttctgtcgta ctctcacctg aagccgcccc tcatcaattg actcctgttg
     4741 taaaatgtgc agatttttca tgaatattat taccttaata tctttttaca ataatatgca
     4801 caaagtgcag cgcatatgat ataatgagcg taggcaacga gcagtgccaa attgaaaata
     4861 tataaaaatg ccagcttgct gagcactttt tatatatttt taatttgata gggcgactgc
     4921 ccgtacagtc aggaaaatct cagatttttc tgacttgcac tgcgtacaat acacttacta
     4981 aggtggttgg gggacgtgct ctcatctgat ccgagtctta cggaaaggat gattattatg
     5041 gtttcttgga acgacttatt tactttcgtt atcatgctgg tagcgattct gacatacata
     5101 gatactcaca gaaaacataa gaaatagccg tcctgcctct ggtaaagtct gggaacggct
     5161 atctcttacg ataacttatt aacttaattt gccggatcag gtgaagtgca ctcacctacc
     5221 gactgcctta ctaagtgtat tatatgtcac ttagcagcat ttgtcaattt tattaatatt
     5281 aaggaggaaa attatgcata catttcagcc atatccaatc gacatgctcg atatcaatcc
     5341 attcaccaag ctcagtaagg agtgggctct cgtgacagcc ggcgacaagg aaaaatcaaa
     5401 caccatgacc gtttcatggg gtggaaccgg tgttctctgg ggcaaaaatg tcgtatttat
     5461 ttttatcagg gaatcaagat atactaagaa tttcatagac aatggtgagt ttttctcact
     5521 ttctttccta agcgagaaat accgcgatgc cttaaaatac tgtggtgctc actcaggacg
     5581 cggagaagac aaatggtcaa aggccggtct gtctcctgct acacgccatg gcataccata
     5641 tccggatgag gcaaatctcg tattcctctg tcgcaagatg gctgctgtgc ctattgaaga
     5701 ggcttcattt acagacaaag ccattatgca tcaatggtac ggcgatcacg atatgcacac
     5761 tatgtatgtt ggtgaaatta ttgaggttgc ggctcgctaa ttttatgaca atactacggg
     5821 acgagaagaa ccctaatatg tatttatgcg cttaaaacta cgtggctaag ataattgtaa
     5881 gaattatgct ttaaggtttt ctatgcttga cgaaaccggc ttttatgcgc cgtccgtggc
     5941 gcataacgcc ttgccctgcc gtccatggca gggcatttct gtgtttcatg gcataattct
     6001 aacaatatct aagtcacttc gtaatcgctt ataaatacat attagggttc ttctcgtccc
     6061 tttctctttg gcaatatata tagattagcc gactctttaa aatgtatcac ggcgtaaggg
     6121 gcaatatctg ccaacagata ttgcccctta ttaataccat aatctagcgg cagccggtac
     6181 tcatagcttc aatggccacg gcaccaccgc gcacaaccga gatatgcttg tactgtgact
     6241 tgagcagggc tataagatca tcattgcggt gctctgtgag cttgctctct aagatcacgc
     6301 tgtcggcatt cttgtccacc acgtcaatat taaaaacctg cgctgtctgg aagatatcag
     6361 tgatttcctc gcgggtgagc tttgataccc tgacatagag tatttccttc atatgcagag
     6421 gcacatcagt caggtcaata accttgatga cctcgaccat tctgttgagc tgctttttta
     6481 tctgctcaaa ggtaatatca tcgcttgtaa cgcaaatagt cattctggaa atagtctcat
     6541 cctctgtggt tccaaccgta agagactgta aattgtagga ttttgcagag aaaagtcctg
     6601 aaacctttgc taaaacaccg acctcattct ccacataaag tgcaatccat ctgtttttca
     6661 tttcaatatt attcatcttc tgcacctcct actttaaaat catttcactc ataggatttc
     6721 cgctcttgac cattggaaga acaatctcat ccgtagctat cataaactca ataattacag
     6781 gtacatcctt atatttcttt gcctctgcaa aggctgcatc gatatcctct tccttatcca
     6841 cgcggattcc gtgtccacca tagctttcaa caagcttgag aaaatccggt gtgtatggag
     6901 gacatgcctc atttggtccc ttgcactcat ccggacagcc ctttcttctt cgaaggcagg
     6961 ttgcagaata acgcttgcca taaaaaagct gctgcatctg gcgcaccatg ccaagatagt
     7021 agttgttgag cacgcatatg gtcactcctg ctccctgtac aactgaggtg gcaagctcct
     7081 gtatattcat ctgaagtcca ccgtcaccgc ttatgcatac aacatccttg tcaggatttg
     7141 caatctttgc acctattgct gccggaagac caaagcccat ggttccaaga cctcccgatg
     7201 taagaagctg gctgttgctg tcaagctcta agtactgtgt agcccacatc tgatgctgac
     7261 ctacatctgt aacgtagatt gcatccttat agttcttatt gatatgctcc ataatcatct
     7321 gaggtgtcat acccttgtct cttctcatgc caagaggatt ttcctcatcc catgccttaa
     7381 tctgcttctg ccactcagca gtctttttcg gctcagccca ctcgtttaac ttgtcgagtg
     7441 ccacattggc atctgcaacg ataggcacat caaccactac atttcgtgaa atagatgcag
     7501 tatccacatc aatatgtaca atctttgctt ttggagcgaa ctcatttaag tcaccggtta
     7561 ttctgtcatt aaatcgtgta cctattgaaa aaagtaaatc acactcactg actgccttgt
     7621 ttgccgcata tttgccgtgc ataccacagt ttcccacata aagcggatga gtggtaggta
     7681 tagctccctt acccattatt gtagtgacaa caggcacatt ctctgcctcc gcaaaacggc
     7741 gcaggttttc atttgccctg gcaacattta taccgcctcc tgcaaggata agcggttttt
     7801 tggcactctt taacatcttg taggcgcgct taagctgtcc gacatgtact gtatcattta
     7861 cacgatatcc tctgatttcc accttatccg gatactctgc cggaccggat gcggtctgta
     7921 tgtcctttgg caagtcaatc agtacagggc ccggctttcc ggttcttgca atatgaaatg
     7981 ccatctttat gatttttcca aggtcctttc tgtcacggac tgtaacagaa tactttgtga
     8041 tatttctggt gattccgact atatccactt cctgaaaggc atcatttccg ataagtggaa
     8101 gcggtacctg tcctgtaaag cacacaagtg gcacattgtc
//
LOCUS       DPUF01000032            8651 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6875, whole
            genome shotgun sequence.
ACCESSION   DPUF01000032 DPUF01000000
VERSION     DPUF01000032.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8651)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6875"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="DHV81_01095"
     CDS             <1..621
                     /locus_tag="DHV81_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HCI93138.1"
                     /translation="RDKNILICPEYHLIVTEGTETEPQYFEAIRTIINSQYRDKIQLD
                     IHGAGDNTLSLLDKAMNLVMNNPNGYKHVWIVYDTDDFPAKRINKTNELCINMSTEET
                     QYHAIWSNQCIELWFLLHFSFVQSDIHRSDYWPKLSEWLKNIGQGEYSKGRADMFQIL
                     RPFMDFAITNAERLDAMNEGKTPASSAPGTKVHELIKHLRPYLKIG"
     gene            645..1307
                     /locus_tag="DHV81_01100"
     CDS             645..1307
                     /locus_tag="DHV81_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCI93139.1"
                     /translation="MMQENERLEKQLDFIREIDKEKEIFRQTYLADASRKENDAEHAW
                     HMAIMTMLLSEYANEKIDVLKTVGMLLIHDIVEIDAGDTYAYDEAGKATQHEREQKAA
                     ERIYGLLPKEQGEPLLELWEEFEAQQTPEARFARTMDNIQPMLLNDASDGLSWREHSV
                     KLSQILGRNKRTALGSEKIWDYAFNNILKKHVESGNIIDDEGVFSAEAGTRAKASERN
                     GR"
     gene            1501..2100
                     /locus_tag="DHV81_01105"
     CDS             1501..2100
                     /locus_tag="DHV81_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HCI93140.1"
                     /translation="MNIVCLDLEGVLVPEIWIAFAKETGIPELEKTTRDEPDYDKLMN
                     YRINILKEHGLGLKEIQETIAKIDPMPGAKEFLDELRSITQVIIISDTFSQFAGPLMK
                     KLGWPTIFCNELEVAEDGEITGFRMRCEQSKLTTVKALQSIGYDTIASGDSHNDLGMI
                     QASKAGFLFRSTDAIKKDYPELPAYETYDELLAAIKAAL"
     gene            2225..2422
                     /locus_tag="DHV81_01110"
     CDS             2225..2422
                     /locus_tag="DHV81_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93141.1"
                     /translation="MYTVTHIYEADFGCEERADGAKAKCVVYLEDENGRQRHVEVEDE
                     WLTANGIEEGSSWKENITDEA"
     assembly_gap    2506..2585
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2618..2836
                     /locus_tag="DHV81_01115"
     CDS             2618..2836
                     /locus_tag="DHV81_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93142.1"
                     /translation="MKKTYKIDVDCANCANKMEDAAKNTAGVKDATVNFMTLKMSVEF
                     EEGQDAKAVMQEVLKNCKKVEDDCEIFL"
     gene            2918..4816
                     /gene="cadA"
                     /locus_tag="DHV81_01120"
     CDS             2918..4816
                     /gene="cadA"
                     /locus_tag="DHV81_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCI93143.1"
                     /translation="MELWRMNKKQKKVLARIIIAAVLMIILHFIPVKGIIRFLLYMVP
                     YLVIGYDILKKAFKGIKNKQVFDENFLMAVATVGAIAVALYENGDYTEAIAVMLFYQI
                     GELFQSYAVGKSRKNISELMDIRPDYANIEVDGKLTQIDPDEVAIGSVIVVQPGEKVP
                     IDGVIVEGSSTLNTSALTGESVPRDAKCGDEIISGCINMSGVLKIKTTKEFGESTVSK
                     ILDLVENSSSKKSKSENFISKFAKYYTPAVCYSALALAILPPIVRILFMSAAPQWGIW
                     IYRALTFLVISCPCALVISIPLSFFAGIGGASREGVLVKGSNYLETLSQTRYVVFDKT
                     GTMTEGVFEVAGVYDNTLDRKKVLEYAALAESSSSHPISRSLQKAYGKEIDRTRVTDV
                     EEISGHGITAKVNGVSVAAGNYKLMKKLGLSYSETDKVGTIVHIAIDGRYEGYILISD
                     KIKPTSAAAIKALKKAGIKGTIMLTGDSRTVADSVAAELGIDEVYSELLPGDKVAKVE
                     ELLARKGSKEKLAFVGDGINDAPVLSRADIGIAMGAMGSDAAIEAADIVLMDDDPLKI
                     AKAIKISRKCLRIVYENTYFAIGIKLICLVLGAVGIANMWLAIFADVGVMVIAVLNAI
                     RALFVHKL"
     assembly_gap    5293..5432
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5444..5788
                     /locus_tag="DHV81_01125"
     CDS             5444..5788
                     /locus_tag="DHV81_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI93144.1"
                     /translation="MECCDCNEIHEDLLKIVNDTMPEETELYDLAELFKVFGDSTRIR
                     ILFVLFEAEVCVCDLAKVLNMTQSAISHQLRILKANKLVNSRREGKSVFYSLADGHVR
                     TIIAQGREHIEE"
     gene            5807..6604
                     /locus_tag="DHV81_01130"
     CDS             5807..6604
                     /locus_tag="DHV81_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HCI93145.1"
                     /translation="MTELEEYYNKFNEEKRLNSRHGRVEFITSMKYIHDCLGSLMNEK
                     QLDLRSQIKILDVGAGTGRYSVPLAEEGYDVTALELVKHNLGRLRQKSDKVKAYQGNA
                     TKLKKFGNDEFDLTLVFGPMYHLKSAEEKLAALNEAKRVTKPGGYILVAYIMNEYSVI
                     TYAIKEKHIKEGIEGGMLDESFHCTEKANDLYSFVRLEDIEALNAQAALTREKIIAAD
                     GAANYIRPFLNALDEEEFDMFVQYHLSTCERADLMGASAHTVDILRV"
     gene            6623..>7811
                     /locus_tag="DHV81_01135"
     CDS             6623..>7811
                     /locus_tag="DHV81_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HCI93146.1"
                     /translation="MKNKGLKILLSVLLVLCIIMAGALIGKEYIGDNIKEAANQNGVD
                     VVKDTSNAGKAEDNAVDSTAEGKESTQSDAAKAEATEADKQQPQMSTIVITATGDCTL
                     GNNQEQSYDGSFNSYYDSKGQDYFFGGVRDIFEADDMTLINLECVLSDATERVEKRWN
                     LKGKPSYIGIMTGSSIEACSLGNNHTYDYGQAGLDDTRNVLDNAGIVYGFNDHTGIYE
                     TADGTRIGIVSVSLLSQNGDREAYIQNGIAQLREQGAAIVIACCHWGIEGDHYPNDYQ
                     QAAAHRIIDWGADLVVGNHPHVLQGMEVYNGKMICYSLGNFCFGGNKNPADKNTAIYQ
                     QAFTLINGELQPGIDAQIIPCTLSSTSAYNDFRPTVASGEKAQEICNLMNTYSQNCSN
                     IEID"
     assembly_gap    7812..7928
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7972..>8651
                     /locus_tag="DHV81_01140"
     CDS             7972..>8651
                     /locus_tag="DHV81_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HCI93147.1"
                     /translation="MYMWRERSKEEKHEDVVNTGLLPLDVVEGFKIRPGFFRMYGACV
                     ASNGVSFTINSHGATRCTLLLFKPQAPKPYARIPFPDSYRIGDTYSMLVYDIKPDEFE
                     YAFSFDGPYEPAKGLLFNEENVLLDPYSRAVTGQRKWGEKPEGGKDFEYRARVVKSSF
                     DWGNIKQLEQPFEDLVIYETHVRGYTKDKSSGVSAPGTFAGLKDKIPYLKDLGINAVE
                     LMPIFEFD"
BASE COUNT         2719 a         1572 c         2086 g         1937 t
ORIGIN      
        1 cgagataaga atatattgat atgtccagag taccatttga tagtaacaga aggaactgaa
       61 acagaaccac agtatttcga agcaatcagg acaatcataa acagtcagta tcgagataaa
      121 attcagcttg acattcatgg tgcaggagac aatacgctta gtcttttgga taaggcaatg
      181 aatctggtta tgaacaatcc gaatggatat aagcatgtct ggatagtcta tgatactgat
      241 gattttccgg caaaacgcat caataaaacg aatgaattat gcattaatat gtctacagag
      301 gaaacacaat atcatgctat ctggtcaaat cagtgtattg aactttggtt tttgcttcat
      361 ttcagttttg ttcagtcaga tattcataga agtgattact ggccaaaact atctgaatgg
      421 ttaaagaata ttggacaggg agaatattca aagggaaggg cagatatgtt ccaaattctg
      481 agacctttca tggattttgc aataacaaat gctgaacgct tggatgcaat gaatgaagga
      541 aaaactcctg caagttcagc tccaggcaca aaagtacatg aattgattaa acacttgaga
      601 ccatatttaa aaatcggata ggataggaag aaggaaccag taaaatgatg caggaaaacg
      661 aaagattaga aaaacagctt gatttcatta gagaaatcga caaggaaaag gagatattcc
      721 gccagactta tttagctgat gcaagccgca aggaaaatga tgcggagcat gcctggcata
      781 tggctatcat gaccatgctc ttgagcgagt atgccaatga gaagatagac gtgctaaaga
      841 cagtaggaat gcttttgata cacgacatag tagagattga cgcaggagac acatatgcct
      901 atgatgaggc aggcaaggcc acacagcatg agcgtgagca gaaggcggcg gagcgtatat
      961 acggactctt gccaaaggaa cagggcgagc cgcttttaga gctgtgggag gaatttgagg
     1021 cgcagcagac accggaagcc agatttgccc gcaccatgga caatatccag ccaatgctcc
     1081 taaacgatgc atcggacggg ctttcatggc gtgagcacag tgtaaagctg tcgcagatac
     1141 tcggcagaaa taagcgcaca gcacttggct cagagaaaat ctgggattat gcgtttaaca
     1201 atatactgaa aaagcatgtt gagagcggaa atatcataga tgatgagggc gtcttctctg
     1261 cggaagccgg tacgcgtgca aaagccagcg agcgtaatgg acggtaataa tatttactga
     1321 aaaatccttt gaaaatcaca cataattcta ttgtagacag tcgtcttgta tgatatactt
     1381 tactggatta aatcggtgaa ctgaatatca ggtaactatt caaggctgct taaatagtta
     1441 cctgacaagt atattgagct tcatcgaaat ccacagaata aaacacacgg aggaatcaaa
     1501 atgaatattg tatgcttaga ccttgaggga gtattggtac ctgaaatctg gattgcattt
     1561 gcaaaggaga caggcattcc tgagcttgaa aagacaacaa gagatgagcc tgattatgat
     1621 aagcttatga actatagaat aaatatttta aaggagcacg gactcggatt aaaagaaatc
     1681 caggagacaa tcgcaaagat agatccaatg cctggcgcaa aggagttttt ggatgagctt
     1741 cgttcaatca cacaggtaat catcatcagt gatacatttt cacagtttgc aggccctctc
     1801 atgaagaagc tcggctggcc gacaatcttt tgtaacgagc ttgaggttgc tgaagatggc
     1861 gagattacag gctttagaat gagatgtgag cagtcaaagc ttacaactgt aaaggcactg
     1921 cagtctatcg gatacgatac catcgcaagc ggtgacagcc acaacgattt aggcatgata
     1981 caggccagca aggcaggttt ccttttcaga agcacagatg ccatcaagaa ggattaccct
     2041 gagcttcctg catatgagac atatgatgag ctgcttgcag cgattaaagc ggcattataa
     2101 tcataaatat agttgaaaaa agcttcccac atgatatttt ggagtggata catccacctg
     2161 tacatgtggg aagtttctat atatttatgt catgcaaaat catatgaaag aaggtagagt
     2221 gcagatgtat acggtaacac acatatatga ggctgatttt ggctgtgagg aaagagcaga
     2281 cggcgcaaaa gcaaaatgcg tggtttatct cgaggacgaa aacggcagac agcgtcatgt
     2341 agaggttgag gatgaatggc ttacggcaaa tgggatagaa gagggaagca gctggaaaga
     2401 aaatatcaca gatgaagcct gaatcataaa ctatgagatt aattatgtga atagagtatg
     2461 tgaataaatt ttatgtttag atgcacattg tgtattgaca acagtnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnaaata attcatgaaa ataaggagaa caccactatg aagaagactt acaaaatcga
     2641 tgtagactgc gctaactgcg ccaacaagat ggaggatgcc gcaaagaata cagcaggtgt
     2701 aaaggatgct acagtcaatt tcatgacact caagatgagc gttgagtttg aagagggaca
     2761 ggatgcaaag gcggttatgc aggaggttct taagaactgt aaaaaggtag aggacgactg
     2821 cgagattttc ttataaattg ccgggcgcag gaatgacaaa cgatattatt tcaatgcctg
     2881 cgctatagta agggtgatta tcaagagatt ataatgaatg gagctatggc gtatgaataa
     2941 aaagcagaaa aaagtactgg cgcgtatcat aatcgcagcg gtactcatga ttatattaca
     3001 tttcataccg gttaagggaa ttatcaggtt cctgctttac atggtccctt atctggttat
     3061 aggatacgat atcctgaaaa aagcatttaa gggaatcaaa aacaagcagg tatttgatga
     3121 aaacttcctg atggcggttg ccactgtagg tgcgatagcg gttgcacttt atgagaatgg
     3181 tgactataca gaggctatag cagttatgct cttctaccag ataggagagc tgtttcagag
     3241 ctatgcagtc ggaaagagcc gtaagaatat cagcgagctc atggacatca gacccgacta
     3301 tgccaatatc gaggtggacg gaaagctcac ccagattgat cctgatgagg ttgctatcgg
     3361 ctcagtaatc gtggtacagc ccggagaaaa ggttcctatc gacggtgtga tagttgaggg
     3421 ttcatctaca ttaaacacca gcgcgcttac aggtgagagc gtgccgaggg atgcaaaatg
     3481 cggtgatgag ataatcagcg gatgcatcaa tatgtccggt gttttaaaga ttaaaaccac
     3541 caaagagttt ggcgaatcaa cggtttcaaa gatacttgat ctcgttgaaa attcaagctc
     3601 aaagaaatca aaatcagaaa acttcatatc aaaatttgcc aagtattata ctccggcagt
     3661 ctgctacagt gcacttgcac ttgcgatact gccgccgatt gtcagaatac ttttcatgtc
     3721 agccgcacca cagtggggca tatggatata cagggcactt accttccttg taataagctg
     3781 tccgtgcgcg ctggtcatca gtattccgct cagctttttc gcaggaatcg gaggcgccag
     3841 ccgcgagggt gttcttgtaa agggctcaaa ctatcttgag accttatcac agaccagata
     3901 cgttgtattt gacaagaccg gcaccatgac agagggcgtt ttcgaggtgg caggagtcta
     3961 cgacaatacg ctcgatagaa aaaaggtgct cgagtacgca gcacttgcag agagcagctc
     4021 gtcgcaccca atcagcagga gcttacagaa ggcgtatgga aaggaaatcg acagaacccg
     4081 cgtgacagac gttgaggaaa tcagcggcca cggaatcacc gcaaaggtaa atggtgtatc
     4141 tgtagcggca ggaaactaca agctcatgaa aaagctgggc ttaagctaca gtgaaactga
     4201 caaggtcggc accatagtcc atatcgcaat agacggcaga tatgagggct atatccttat
     4261 ttcagataag ataaagccaa cctcagcggc agcaataaag gcattgaaaa aagccggaat
     4321 aaaaggtaca ataatgctta caggcgattc gaggacagta gccgactcag tagcggcaga
     4381 gctgggaatt gatgaagtat acagtgagct tttaccgggt gataaggtgg caaaggtgga
     4441 ggaactgctt gcaagaaagg gcagcaaaga aaagctcgcc ttcgtcggag acggaatcaa
     4501 cgatgcaccg gtgctctcaa gagccgatat cggaattgca atgggcgcta tgggctcaga
     4561 tgcagcaatc gaagccgcag atatcgtcct catggatgat gacccgctaa agatagcaaa
     4621 agcaataaaa atatcgcgca aatgcctgcg aatagtatat gaaaacacat acttcgcaat
     4681 aggtatcaag ctgatctgcc tcgtgctcgg agcagtcgga atagccaata tgtggctcgc
     4741 aatatttgca gacgtaggag taatggtgat agcagtgcta aatgcaatcc gcgcattatt
     4801 cgtacacaag ctctaaaaag gtagagctca aagagcagat atcaaagcat gatgaaacta
     4861 ctatataaaa aataccagac agaatgcacc caagagatgc actctgtctt tcgcgtataa
     4921 taaaagaaat tactgttcac acgaaagtgt gacagtaaca aaagacaatt aaacggcaat
     4981 agctaataaa caaaagctaa gtaaattcct gcagtgccct tacacgcatc cggaccagcc
     5041 ccgtaactgc gaaggcactg agcagttacg ctaccgccaa tacgacgcgt caatacctta
     5101 aatgagccaa aaactaaggg tgcttgcaga aattgcatgt gacatcgcga atatggagat
     5161 atttagagtc tgttggaatt atgtcaaaac acagcaatgt ccgtgcatgg acgcacggac
     5221 aaggcgttag cgccacggac ggcgctaaaa gccggttgcg tcctatagcc aaaacctaaa
     5281 tcataattcc tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaaatgaa cagatagaat gctgcgactg
     5461 caacgaaatc cacgaagacc ttctgaaaat agtaaatgat acaatgcctg aggaaaccga
     5521 gctctacgac cttgcagagc tttttaaggt atttggcgac tccacccgga tcagaatcct
     5581 ctttgtgttg tttgaagcgg aggtatgcgt ctgcgacctt gcaaaagtgc tcaacatgac
     5641 acagtccgcc atatcacatc agcttaggat attaaaggca aacaagctcg taaacagcag
     5701 acgtgagggc aaatcagtgt tttattcact tgccgacgga catgtgcgca caatcattgc
     5761 gcagggacgt gagcatatcg aggaataata cagggaggaa tataacgtga cagaattaga
     5821 agagtactat aacaaattca acgaggaaaa gcgcctgaac agccgccacg gcagggtaga
     5881 gtttatcacc tccatgaagt atatacacga ttgtcttggc agcttgatga atgagaagca
     5941 gcttgatctt cgctcacaga tcaaaatact ggatgtcggt gcgggaacgg gcagatactc
     6001 agtgcctctt gccgaggagg gctatgatgt gacagcactc gagttagtta agcataacct
     6061 cggcaggctg aggcaaaaga gcgataaagt aaaggcatac cagggcaatg ccacaaagct
     6121 taaaaagttt ggaaacgatg aatttgacct cacactcgta ttcggaccta tgtatcatct
     6181 gaagtcggca gaggaaaagc ttgccgcatt gaatgaggca aaaagggtga caaaaccggg
     6241 tggatacata cttgtggcat acattatgaa tgagtacagc gtgataacct atgccataaa
     6301 ggagaagcat ataaaagagg gcatagaggg tggaatgctc gatgagagct tccattgtac
     6361 agaaaaagct aatgatttgt acagttttgt ccgattggaa gatatagagg cattaaatgc
     6421 acaggcagcc cttacacgcg aaaagataat agcagccgat ggcgcggcaa attatatcag
     6481 gccatttttg aatgctcttg acgaggaaga gtttgatatg tttgtacagt atcatctttc
     6541 aacatgtgag agagcagacc tgatgggagc aagtgcgcac acggttgata ttctaagggt
     6601 atgaggaata tgaggagaaa ttatgaaaaa caagggatta aaaattctat tatctgtgct
     6661 tttggtttta tgcatcatta tggcaggagc actcatagga aaagaatata ttggagataa
     6721 tataaaggaa gccgctaacc agaatggtgt ggatgtagtt aaagacacat cgaatgccgg
     6781 aaaagcagag gataacgcag ttgacagtac ggcagaaggc aaagagagta cacagtctga
     6841 tgcagccaaa gctgaggcaa cagaagcgga taaacagcag ccacagatgt caaccatagt
     6901 gattacggct acaggagact gtacactcgg caacaatcag gagcaaagct atgacggcag
     6961 ctttaattct tattatgact caaagggaca ggactacttt ttcggcggcg tgagagatat
     7021 ttttgaggct gacgatatga cactcatcaa tctggagtgt gtgctatcgg atgcgacgga
     7081 gcgcgtagaa aagcgctgga accttaaggg aaagcctagc tacattggaa taatgacggg
     7141 cagctccatc gaggcctgca gtcttggaaa caatcacaca tacgactatg gtcaggctgg
     7201 actggacgat acgagaaatg tgctcgacaa tgccgggata gtatatggct tcaatgatca
     7261 taccggtatt tatgagactg ctgatgggac aaggattgga atagtgtcgg tcagcctttt
     7321 gtcacaaaac ggtgaccgag aggcatatat ccaaaacggt atagcgcagc ttcgtgaaca
     7381 gggagcggcg atagttatag catgctgcca ctggggcata gaaggcgacc attaccctaa
     7441 tgactatcag caggcggcgg cacaccgtat cattgactgg ggagcagatc tggttgttgg
     7501 aaaccaccct catgtactgc aggggatgga ggtctataac ggcaaaatga tatgctacag
     7561 tcttggaaat ttctgcttcg gaggcaacaa aaatccggca gacaagaaca cagcgattta
     7621 tcagcaggca tttactctga taaatggtga attgcagccg ggcatagatg ctcagattat
     7681 tccatgcacc ttaagcagca cgagcgcata caatgatttc agaccgacag tggcttctgg
     7741 cgaaaaagct caggagatat gcaatttaat gaacacatat tcacaaaatt gtagtaatat
     7801 agagattgat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnngc atgtaccatc atacaaatat agtaagggag caaagagcaa tatgtatatg
     7981 tggagagagc gcagtaaaga agaaaagcat gaggatgtag taaatacagg cctgctgccg
     8041 cttgatgtgg tagagggatt taagatacgt ccgggctttt tcagaatgta cggagcgtgc
     8101 gtggcatcga acggagtgag ctttaccatc aattcccatg gagcaacaag gtgcactctt
     8161 ttattgttca agccacaggc accaaagccg tatgcgagga ttccgtttcc tgattcatat
     8221 aggataggag atacctactc catgcttgtt tatgatataa agccggatga atttgagtat
     8281 gcattttctt ttgacgggcc ttatgagccg gcaaagggac ttttgtttaa cgaggaaaat
     8341 gtccttttag acccatattc cagagcggtc acaggacagc gaaaatgggg agaaaaaccg
     8401 gagggtggca aggattttga gtacagagcc agagttgtaa aaagcagctt tgactggggc
     8461 aacataaaac agcttgagca gccctttgag gaccttgtga tttatgagac acatgtgagg
     8521 ggctatacaa aggataagtc atctggagtc agcgcaccgg gcacatttgc cggattgaag
     8581 gataagattc cgtatcttaa ggaccttgga ataaatgcag tggagctcat gccgattttt
     8641 gagtttgatg a
//
LOCUS       DPUF01000033            8449 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7283, whole
            genome shotgun sequence.
ACCESSION   DPUF01000033 DPUF01000000
VERSION     DPUF01000033.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7283"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="DHV81_01145"
     CDS             <1..165
                     /locus_tag="DHV81_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCI93148.1"
                     /translation="QGQTGYVSSDYVKSDMADSTTDSGTSGAAGSGAASQGTADGSTT
                     GAADNAAQSQ"
     gene            190..1023
                     /locus_tag="DHV81_01150"
     CDS             190..1023
                     /locus_tag="DHV81_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93149.1"
                     /translation="MTGIIWACAATFIAACVPILAKVGTKKTDPALAGSIAGIVLCAS
                     IYFYRKDNIMGSSLIALGQRSVIFILLAGLATGLFGICFFKSIHEGYTFQVTAIVKCS
                     YIITLAAGFFLFHNTPDTFDYIAIALMIVGTVILNLDGTGILFALLAAVCASAAHILV
                     SLGGIQLDKGFIAFWCVFIGTLVLIIFTIATGGMKKIRSMSFVDGICLILAGIAYPLA
                     YDFYGRAVSMSGSYAGYIFNLTIIVMMICSSVILKEKVSGKKFLGACIFIAALFVIAC
                     N"
     gene            1065..1406
                     /locus_tag="DHV81_01155"
     CDS             1065..1406
                     /locus_tag="DHV81_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93150.1"
                     /translation="MLPFYKTFPFMDYYKNEDAFAKDLGMIKDMYPHDTAMIQSAVEQ
                     RMDELEYEGSFIYDEVPDRQRLFDEADRIAGRFISEQSSRCFICTIAGILFNCELCRR
                     RCRYRRYQRFW"
     gene            1425..3953
                     /locus_tag="DHV81_01160"
     CDS             1425..3953
                     /locus_tag="DHV81_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93151.1"
                     /translation="MSKKVIKPIVLIVVFIAALITFCITTNKGNKDMTTKQADATLPV
                     MSFNLDKIKINTLHGYTTEMDPTKMRDCVIPISDDRKLSLSISTYGMAVDRISYKIRS
                     MDGKRLVADGEISSFSNKDNTIQADVSMPNVMDENTEYLLVFTITSGQDNVYYYSRIM
                     QTDGKAAAKVVEFAKKFHDETFIKDDKSFFTTYMETTTGDRNTLAHVDLTSTVSQITW
                     GSMAAAQYTNPVIALKEINDSYDVVTIDYVMSCVDGKGETEYYNVREYFRLRQTESRM
                     YVLNYERTANQIFNSENSFISDSGSVMLGIRSSEAEYRANEAGSVICFVQEGDLYSYD
                     INNGMIIKVFSFRDAEGIDERENWNHHDIKIVSVDEAGSIDFVVYGYMNRGTHEGEVG
                     TGVYHYDGLAHTIDEEAFIPSKTSYEVLKAEMGKMLYLNEKNEFYLMMDDSLYRINLG
                     SMSVKKVVEGLSTGSYCASESNRYFAWVDSANQYSSNTIKVMDLKSGKTFEVKKGDDQ
                     YLRPLGFIGEDFIYGQANAADVVSDAAGNTTFPMNGLIILDTSDQSELKTYTPSGGYV
                     EKISVDGYTVTIDLIAQNNGVYAEIGQDTIMNREADSKQKIALDTSQSDTKLTVSAIS
                     IAGGKKPDKLKQLTAQMTINSHDTTVDLKFDDNTVHFYVYAKGDVIFASDNISDAIKQ
                     ANDSMGVVIDSNQQYVWMRARKNAVNAFANIACNETDKDADSVVKSVSAMLTYNDVTV
                     SVSELIGAGSSAVDVLKNNLPDKEILDLQGVSSEDIIFYISQGNPVFAMTGNTSAVLV
                     TGYSSNGALYIYNPDNGATTSMSYEDADRMFYNGGLHFITYMTK"
     gene            4113..4367
                     /locus_tag="DHV81_01165"
     CDS             4113..4367
                     /locus_tag="DHV81_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCI93152.1"
                     /translation="MSKKSIVVTKVCEGHDNPIAELVQVACKFDSEITLESDNRKINA
                     KSIMGIMAFNPSDGMSVDIVTKGDDEQEALVAIENFLVCE"
     gene            4390..4905
                     /locus_tag="DHV81_01170"
     CDS             4390..4905
                     /locus_tag="DHV81_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93153.1"
                     /translation="MRKKNNSKTAKPAVKPVEVKKEETVAQPVSEPKADAKADVKAEV
                     EKAVEKTTEKVEKAVEKTTEKVAEKAAEVKADVKDTVKKTTAKAAKAAKTAKEAKTAK
                     KEPVKPEIIVQYQNSEVDTAAVEERVKAQFVAEGHKAGFIRKLNIYIKPEEYSAYYVI
                     NDKFSGRVDLF"
     gene            complement(5069..5139)
                     /locus_tag="DHV81_01175"
     tRNA            complement(5069..5139)
                     /locus_tag="DHV81_01175"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(5105..5107),aa:Gly,seq:tcc)
     gene            complement(5166..5238)
                     /locus_tag="DHV81_01180"
     tRNA            complement(5166..5238)
                     /locus_tag="DHV81_01180"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(5203..5205),aa:Phe,seq:gaa)
     gene            5482..6543
                     /locus_tag="DHV81_01185"
     CDS             5482..6543
                     /locus_tag="DHV81_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCI93154.1"
                     /translation="MLRELRIGDMVAKLPIIQGGMGVGVSLSRLAGAVAKEGGVGVIS
                     TAQIGFEEPEFEKDQAKANLIAIKKHIKKAKELACGHGMVGVNIMVALKHYKEHVLAA
                     VEAGADVIISGAGLPMELPELVDEKSHTRIAPIVSSKRAANLILKMWAHRYNRTADFI
                     VIEGPKAGGHLGFSNEQLADMEHMDYDSEIKEIISCAKTYEEKFKKHIPVIVAGGVFT
                     HEDVMHCIELGADGVQVASRFVATEECDASDAYKHAYLNANESDVQIIQSPVGMPGRA
                     VRNEFIRGLEKEKQHITKCYNCLEKCNPATVPYCITKALIAAVKGDMQNGLVFCGANV
                     GRINRMTTVHELMSELVGA"
     gene            complement(6518..>8449)
                     /locus_tag="DHV81_01190"
     CDS             complement(6518..>8449)
                     /locus_tag="DHV81_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCI93155.1"
                     /translation="INRTHQGYLIASLHAPVQRYVCFSKTNEKIVTDSRTYDLSSDQF
                     KGGCHTDGIQYIRDFTYDGTICFTYEAGDITIKKHIALAQGKNLAAVAYEVQNKGEAA
                     KLLITPLMNFREHSESSTVDSLKFEVQKQEQGFTLIPKAQDDHCIRIVFSGGELIERD
                     DKYICDLEAQTEVDNEVPGLDCAFCPYDVSVDIPAGESMHFSVMCDIVPLEACNGNAG
                     SALAKHLVSDNESAFEILHAQLDYTDELIKRSGFTDDFAVKLTVAANQFIAHRQSTGY
                     DTVLAGLPWFTDWGRDTMISYTGLTLCTGRFEKAHDILLTFAKYCKDGLIPNMFPDSG
                     LEPLYNTVDASLWYFYAVYKYLEYVSTPDAYEFIKKDIYPCLKDIISTYKKGTDFSIY
                     MDTDGLIHAGSGLDQVTWMDVRVGDWVATPRHGKPVEINALWYNALCTMDMLQAKFGD
                     NDDSYRQLASLVKTSFNKRFWNENTGCLYDVVDEDDDTIRPNQIYAVSLPFTMLDRER
                     EKAIVDTVMDKLYVGCGLRSLDPAHKDYHPIYIGSLSKRDHAYHQGTAWGFLLGGFIS
                     AYVKVNGRTRATALCADKLLDPVREHLLNNCIGSICEIFDGDAPHNGRGCYAQAWSVG
                     EVLRCYCEDVLPMLPQAHS"
BASE COUNT         2513 a         1528 c         2144 g         2264 t
ORIGIN      
        1 cagggacaga caggctatgt cagctctgac tacgtcaagt cagatatggc tgacagcact
       61 acagacagcg gcacatcagg tgcggcaggt agcggggctg ccagtcaggg tacagcagat
      121 ggcagtacaa caggagctgc agataatgct gcacagtcac agtaatagat acgtggtgag
      181 gtaatgtata tgacaggaat tatatgggca tgtgctgcaa cgtttattgc agcatgtgtg
      241 cctattttgg ccaaggttgg aactaaaaag acagacccgg cgctggcggg aagcattgca
      301 gggattgttt tatgtgcgtc tatatatttt tacaggaaag ataatataat gggaagcagc
      361 ctgattgcac tcggccaaag gagcgttata tttatacttc tggcagggct tgcaacaggg
      421 ctttttggca tctgtttttt taaatccata catgagggct acacatttca ggttacagct
      481 atagttaaat gcagctatat aatcacgctt gcagcgggat tttttctgtt tcataacact
      541 cctgacactt ttgactatat agcgattgca ctcatgattg ttggaacggt tattttaaat
      601 ctggatggaa caggtatttt gtttgcgctt cttgctgcag tttgtgcgtc ggcagcacat
      661 attctggtgt cacttggcgg gatacagctg gataagggct ttattgcgtt ctggtgtgta
      721 tttataggaa cgctggtgct tattatattt actattgcaa caggaggcat gaagaaaatc
      781 agatccatgt catttgttga tggtatatgc ctgattcttg caggcattgc atacccgtta
      841 gcatatgatt tttatggcag ggcagtttca atgtccggca gctatgcagg gtatattttt
      901 aatttgacga ttattgtgat gatgatatgc tccagcgtga tactgaagga gaaggtatcg
      961 ggaaagaaat tcctgggcgc atgcattttt attgcagcat tgtttgttat agcatgcaac
     1021 taatagctga atatagcata taatgattaa aaagaggttt tttgatgtta cctttttata
     1081 aaacatttcc gtttatggat tactataaaa atgaggatgc atttgcaaag gacctgggga
     1141 tgataaagga tatgtatccg catgatacgg cgatgataca gtctgcagta gagcagcgca
     1201 tggatgagct ggagtatgag ggcagcttta tctatgatga ggtgcctgac aggcagcgtc
     1261 ttttcgatga ggcagacagg attgccggca gatttatctc agagcaaagc agcaggtgtt
     1321 ttatctgtac aatagcgggc atacttttta actgtgaact ctgcagacgc agatgcagat
     1381 acagaaggta ccaaagattc tggtagatat aaggatatga ttttatgagt aagaaagtga
     1441 taaagccgat agtacttata gtagtattta ttgcagcatt aattacattt tgcataacta
     1501 caaacaaggg aaataaggat atgaccacga agcaggcgga tgcaacgctg ccggttatgt
     1561 catttaatct ggataaaata aagataaaca ccctccatgg atatactacc gagatggatc
     1621 cgacaaagat gcgcgattgc gttattccaa tttcggatga caggaagctc tcattatcca
     1681 tttcaacata tggcatggct gttgacagga tatcatacaa gatcagaagc atggatggca
     1741 agaggctggt ggccgatggc gagatatctt ctttttctaa taaggataat actattcagg
     1801 cggacgtaag catgccgaat gttatggatg aaaatactga gtatcttttg gtgtttacca
     1861 tcacaagcgg tcaggataat gtatattatt acagcaggat tatgcagacc gatggaaagg
     1921 cagctgcaaa ggttgtcgag tttgcaaaga agtttcatga tgagacgttt ataaaggatg
     1981 acaaatcctt ctttacaaca tatatggaga caacaaccgg tgacagaaat acgcttgcac
     2041 atgtggatct gacatctacg gtgagtcaga ttacctgggg ctcaatggcc gctgcacagt
     2101 atacgaatcc tgtcatagcc ctaaaggaaa taaacgattc ttatgatgtg gtcacaatag
     2161 actatgtgat gagctgtgtg gacggtaagg gtgagactga gtattacaat gtgagagaat
     2221 atttcaggct gcgccagacg gaaagccgta tgtacgtgct caactatgag agaacggcaa
     2281 atcagatttt taacagtgaa aacagcttta tatcggattc aggcagcgta atgcttggca
     2341 tcagaagcag tgaggcagag tacagggcca atgaagcagg cagtgtgata tgctttgtcc
     2401 aggagggaga tttatactcc tatgatatca acaatggaat gataatcaag gtattcagct
     2461 tcagggacgc tgagggcatt gatgagcgtg agaactggaa tcatcacgat ataaagattg
     2521 tcagtgtaga cgaagcgggc agtattgatt ttgtggtata cggatatatg aacagaggca
     2581 cccacgaggg agaggtggga accggtgtgt accattatga cggtctggca catactatag
     2641 atgaggaagc gtttattcct tctaagacat catatgaggt gctaaaggct gagatgggca
     2701 agatgcttta tctcaatgag aagaatgaat tctatctcat gatggatgac agtctctata
     2761 ggattaattt aggctcgatg tcagtaaaga aggtggtaga gggcttgtct acaggctctt
     2821 actgcgcatc ggagagtaac agatactttg catgggtgga ttctgcaaat cagtactcat
     2881 caaataccat aaaagtgatg gatttaaaga gtggcaagac ctttgaggtc aaaaagggcg
     2941 acgaccagta tctgcgtccg cttggcttca taggtgagga cttcatatat ggacaggcga
     3001 atgctgctga tgtggtctct gatgcagccg gaaacacaac atttccgatg aatggcctta
     3061 ttattcttga tacatctgac cagtcggagc ttaagacata taccccttcg ggcggctatg
     3121 tcgagaaaat ttctgtggat ggatatacgg taacgataga tctgattgca caaaacaatg
     3181 gtgtgtatgc agaaatcggt caggatacca ttatgaaccg ggaggctgat tcgaagcaga
     3241 agatagctct tgatacgagc cagtccgata caaagctgac ggtcagcgct attagtatag
     3301 caggtggtaa aaagccggat aagctgaagc agctgacagc acagatgact ataaattcgc
     3361 atgatactac tgttgattta aagtttgatg acaatacagt ccatttctat gtttatgcaa
     3421 agggggatgt gatttttgca tctgataaca tctcggatgc cataaaacag gcaaatgaca
     3481 gcatgggagt ggtaattgac tcaaaccagc agtatgtatg gatgagggca agaaaaaatg
     3541 cagtaaatgc atttgcgaat attgcatgta atgaaaccga taaggatgct gacagtgtcg
     3601 tgaagtcagt aagtgcaatg cttacctaca atgatgttac ggtaagtgtg tctgagctga
     3661 ttggagcagg cagcagtgct gttgatgtgc tcaaaaacaa cctgccggac aaggaaatcc
     3721 tggatttaca gggagtgtct tctgaggata ttatctttta tatcagccag ggcaatccgg
     3781 tatttgcgat gacgggaaat acgtcggcag tgcttgtgac aggatactct tcaaatggag
     3841 ctttatatat ttataatccg gataatggcg caacaacatc gatgagctat gaggatgctg
     3901 acagaatgtt ttataatggt ggcttgcatt ttatcacgta catgacaaaa taaacagaat
     3961 aatgttgtaa taatgccgat tttgtgacat taaatgtaag ctccttaggt aaagtttaac
     4021 agaaattttc ataaggattg aaaagcggtt acaattggga tatattaaat aagtatgaaa
     4081 caggtttaat actttttgtt taaggagaac gtatgagtaa gaagagtatt gtcgttacaa
     4141 aagtatgtga aggtcatgat aatccaatcg cagagcttgt tcaagtggca tgcaagttcg
     4201 atagtgagat tacattggag agtgacaatc gcaaaatcaa tgcaaaaagc atcatgggta
     4261 ttatggcatt taatccttca gatggaatgt ctgttgatat tgttacaaag ggtgatgatg
     4321 agcaggaagc tttagttgca attgaaaatt ttttagtatg tgaataatga gcatgaggag
     4381 gaaaatagta tgcgtaaaaa gaataactca aagacagcaa agccagcagt aaagccggtt
     4441 gaagtaaaaa aagaggaaac agtggcacag ccggtttcag agcctaaggc tgatgcaaaa
     4501 gccgatgtaa aggctgaggt agaaaaagcc gtagagaaga caaccgagaa ggttgagaag
     4561 gctgttgaaa agacaactga gaaggttgct gagaaggccg ctgaggttaa ggctgatgta
     4621 aaggacactg tcaagaagac aacagcaaag gcagcaaaag ctgcaaagac agctaaagag
     4681 gcaaaaactg caaagaagga gccggtaaag ccggagatta ttgttcagta ccagaacagc
     4741 gaggttgata cagcagctgt cgaggagcgt gttaaggctc agttcgttgc tgagggacac
     4801 aaggcaggct tcatcagaaa gcttaacatc tacatcaagc cggaggagta ttctgcatac
     4861 tatgttataa atgataagtt ttcaggtcgt gtagatttat tctagtcaga cattattagg
     4921 gaatacatat tttatctatc gcataataaa agccgataca tttcgtggag acatggtgta
     4981 tcggcttttg ttttgtgtat acgtatgtgt aaaagcagct gtatgacagt ttaaatgcat
     5041 tatgaaatac ttagtggaca aacaaaaaag cgggtgatgg gaatcgaacc cacgtatcca
     5101 gcttggaagg ctggtgttct accattgaac tacacccgca taggaatatt taatttgttg
     5161 tataatgccc agttccggaa tcgaaccaga gacacacgga ttttcagtcc gttgctctac
     5221 caactgagct aactgggctt gtctcacaac aaaaattatt ctacacgatt tgtctgttga
     5281 tgtcaatatt aaaatcgaga aaaattggat agttacaata aacaagaaat gtagtatcaa
     5341 aaactatata aaatagaaaa tgaaataatt ttaccttgac atgaatacta taaagggtta
     5401 ctatgtgtat atgtttgatt atcaaataat ttgaatatca aacagaaaaa tggaatttat
     5461 acatgttcgg aggtacatat tatgttgaga gaactgagaa tcggagatat ggttgcaaag
     5521 cttcctatca ttcagggagg catgggagtt ggagtgagtc ttagcagact ggccggagct
     5581 gttgctaagg agggtggtgt aggagtgatt tccaccgcgc agattggctt tgaggagccg
     5641 gagtttgaaa aggaccaggc aaaggctaat ctgattgcta tcaagaagca tattaagaaa
     5701 gcaaaggagc ttgcctgtgg acatggaatg gtaggtgtca atatcatggt ggctttaaag
     5761 cattacaagg agcatgttct ggctgcggtt gaagcaggtg ctgatgttat cataagcggg
     5821 gcaggacttc caatggaact gcctgagctg gtcgatgaaa agtcacatac aaggattgct
     5881 ccgattgttt cttcaaagag agcagcaaat ctgatactta aaatgtgggc acacagatac
     5941 aaccgcacag cggactttat tgttatcgag ggaccaaagg ccggaggaca tctgggattt
     6001 tccaatgaac agcttgcaga tatggagcat atggattatg acagcgagat taaggagatt
     6061 atcagctgtg caaagacata tgaggagaag tttaaaaagc acattcctgt aattgtagcg
     6121 ggcggagtgt ttacacatga ggatgtcatg cactgcatag agcttggagc cgacggtgtg
     6181 caggtggcga gccgttttgt ggcgacagag gagtgtgacg catctgatgc ctataagcat
     6241 gcttatctga acgcgaatga gtctgatgtg cagattatac aaagccctgt aggaatgccg
     6301 gggcgtgctg tcagaaatga gtttatcagg ggattggaaa aggaaaagca gcatatcaca
     6361 aagtgctaca attgtcttga gaaatgtaac cctgcaacag tgccatactg tattacgaag
     6421 gcactgattg cagcggttaa aggagatatg caaaacggtc tggtattctg cggtgccaat
     6481 gtgggccgca taaaccggat gactacggtc cacgagctta tgagtgagct tgtgggagca
     6541 taggcagtac atcctcacag tagcagcgaa gcacttcacc tacactccat gcctgcgcat
     6601 agcagccacg gccgttgtga ggggcatcgc cgtcaaatat ctcgcatatt gagccgatac
     6661 agttgttgag cagatgctcc cttacagggt caagcagctt gtcagcgcat aaagcggtcg
     6721 ccctcgtgcg gccgtttacc tttacatagg cactgataaa accaccgagc agaaagcccc
     6781 aggctgttcc ctgatgatag gcatggtcgc gctttgagag agagccaatg taaattggat
     6841 ggtaatcctt gtgtgcagga tcgagcgaac gcagtccaca gcccacgtaa agcttatcca
     6901 ttaccgtatc cacaattgct ttttcacgct ccctgtcaag catggtaaat ggcaggctta
     6961 cggcataaat ctggtttgga cggattgtat cgtcatcctc gtcaaccaca tcatacaggc
     7021 agcccgtgtt ttcattccag aagcgcttgt tgaaggaagt ctttacgaga gaagcaagct
     7081 gtctgtagct gtcatcgtta tcgccaaact tagcctgaag catatccatg gtgcacagtg
     7141 cgttgtacca aagtgcattt atttcgacag gcttgccatg gcgtggtgta gcaacccagt
     7201 cgccgacgcg cacatccatc catgtgacct ggtcgagtcc gcttccggca tgaatgagcc
     7261 cgtccgtgtc catatatatg gaaaaatcag tgcctttttt ataagtagat atgatatcct
     7321 ttaagcatgg ataaatatcc ttctttataa attcataggc atcgggagtg gacacatatt
     7381 caagatattt gtagacagca taaaaatacc acagtgatgc atctacagta ttgtagagag
     7441 gctcaagtcc gctgtccggg aacatattcg ggatgagccc gtccttacag tacttggcaa
     7501 aggtcagtag tatatcgtga gctttttcaa aacggccggt gcaaagtgtc aggccagtgt
     7561 atgaaatcat agtgtcacgg ccccagtctg taaaccaagg aagtccggcg agcacagtgt
     7621 catagcctgt tgactgtctg tgtgcgatga actggtttgc agccacagta agcttcacgg
     7681 caaagtcatc agtgaagccg cttcttttaa tcagctcatc ggtgtagtca agctgcgcat
     7741 gtagtatttc aaaagcactt tcgttatcag aaacaaggtg tttggctaag gctgaacctg
     7801 cgttgccgtt acaggcttca agtggtacga tatcgcacat tacagaaaaa tgcattgatt
     7861 caccggcagg aatatccacg ctcacgtcat atgggcaaaa tgcacagtcg aggcccggca
     7921 cttcattgtc cacctcggtc tgagcctcaa ggtcacagat atatttgtcg tctctttcaa
     7981 taagctctcc acctgaaaaa acaatgcgga tacagtggtc atcctgagct tttggaatga
     8041 gggtaaagcc ctgttcctgc ttttgaacct cgaattttaa tgaatcgacg gtggaacttt
     8101 cagagtgctc acggaagttc ataagaggcg tgatcaaaag cttagccgct tcgcctttgt
     8161 tttgaacctc atatgccacg gcagcgagat tttttccctg tgccagtgcg atgtgttttt
     8221 ttatggtgat atcaccggcc tcatatgtaa agcatatggt tccatcgtat gtgaaatctc
     8281 ttatatattg gatgccgtct gtgtggcatc cgcccttgaa ctggtcactt gagaggtcgt
     8341 aggtgcggct gtctgtgact attttttcgt ttgtctttga gaagcagaca tatctttgca
     8401 caggagcatg aaggcttgca atcagatagc cctgatgcgt acggtttat
//
LOCUS       DPUF01000034           11318 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_5887, whole
            genome shotgun sequence.
ACCESSION   DPUF01000034 DPUF01000000
VERSION     DPUF01000034.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 11318)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11318)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11318
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_5887"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="DHV81_01195"
     CDS             <1..258
                     /locus_tag="DHV81_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HCI93156.1"
                     /translation="DYQVLAIRDYKNDTRKEMTTGKVEPTGLPSSNVLYYELNDNAWC
                     CVRPSGTEPKIKFYFGVKGVSLEDSQAKLDALSAEVLGQFE"
     gene            264..464
                     /locus_tag="DHV81_01200"
     CDS             264..464
                     /locus_tag="DHV81_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit M"
                     /protein_id="HCI93157.1"
                     /translation="MMKIYICPDCGWIRMVSRRKEVECFKCGRPQMRLTNLEFGKYTN
                     MSEKERQDYADAWLYIHRKKEK"
     gene            466..1692
                     /locus_tag="DHV81_01205"
     CDS             466..1692
                     /locus_tag="DHV81_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI93158.1"
                     /translation="MAKKRRRKKRRSGRKKIILFVFEILLLLIVLLVVWAYNKTLGQV
                     KYEDALTASEAGINEDIDDDALANMHGYLNVALFGLDNRSNGSYNEGHSDCIMIASLN
                     YDTKEVQLVSVYRDTYLPIGNGKFAKANAAYANGGAKRAVAMLNSNLDLNITKYVCVD
                     WKALVDAIDDIGGLDLEITKAEMKEINYLIPEVDYTTGYNTPYLEGDGMQHLDGTQAT
                     CYARIRSTSGDDFLRASRQRIVLQAMLDKAKQSDLGSLTEMCKDIMSQISTNFTATEI
                     LQYASYVTKFQMKETTGFPFELTTMNLSSTGDTVIPIDLAQNVSKLHQFLFNETDYQP
                     TETVQSVSDKIAKKTGVTSKTSAFDLSQYNDTVGSDGTEGAKKKNKDKQDSLKNSQSE
                     SENNSGTKSSSDDSDE"
     gene            1896..3104
                     /locus_tag="DHV81_01210"
     CDS             1896..3104
                     /locus_tag="DHV81_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HCI93159.1"
                     /translation="MKTEIKEKITATNQKYLKKFLTEEAPAKLESQLEHMDWSYLDMI
                     HNKEQQRGTFSPLAAMELSEIEANKDKYLATGFDAVKNGKVGAILLAGGQGTRLGFDK
                     AKGMFNIGKTKELYIFEQLVANLMKVTNQTGTWVPLYVMTSEINDSMTREFFEEHDYF
                     GYNKDYVKFFVQEMVPAVDFDGNLLMKSEDSLAMSPNGNGGWFKSLINAGLDKDLKDK
                     GVEWLNVFAVDNVLQQIADPVFVGATIESGCVSGSKVVRKCDPYERVGAMCLENGKPS
                     IVEYYELTPEMAEAKNENGSLQYGFGVILNYLFRVDKLMAIAEKSLPLHVVEKKVPYI
                     DENGTEHKPETPNAYKFETLILDMVYMMDNSLPFEVDREMEFAPVKNATGTDSVETAR
                     ALLEKNGIEI"
     gene            3346..4956
                     /locus_tag="DHV81_01215"
     CDS             3346..4956
                     /locus_tag="DHV81_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-alkenal reductase"
                     /protein_id="HCI93160.1"
                     /translation="MDNNYGNMNDTNNTNNTNSTNNNTNNVNFTMSGNPYNPDESQKA
                     AQTNNTSAESYSQSYNPYSQASAAGQPNTNQSYANQSYANQPYSNQSYNSGTNNNAYG
                     GASAGSTDNSYSRMNMNGQAYQQTAKPKKQRTPRKPGAFGPFAAKTVAAALIFGLVGG
                     GVFTGVSYIGTRAISNSSTASAKLTTTTSGGTKQTSSGNAKDLTDVSSVVDEVMPSIV
                     AITNTGTVTYNSFFGKQSQQSQSCGSGIIVSEDDDYLYIATNNHVVADSEELTVQFDD
                     DSVVKAEIRGTDPDDDLAVVRVKKADLGKDTYSNIKIATIGDSESVAVGSPAIAIGNA
                     LGYGQSVTTGIVSALNRTVTTQDSQTGETVTNNKLIQTDAAINPGNSGGALLNENGEV
                     IGINSVKYSSTEVEGIGYAIPMSVAKPIIESLIQDGKYTNENQAYLGIKGGDVSSEMV
                     AYGFPQGVYVSSVSAGSGAANAGLQEGDIITAVDSTKISSMTELQSALKSYKAGDKVT
                     LTVARQSGRQYEESKVEVTLSSAKDIQQ"
     gene            5097..6866
                     /locus_tag="DHV81_01220"
     CDS             5097..6866
                     /locus_tag="DHV81_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase"
                     /protein_id="HCI93161.1"
                     /translation="MNKQEKDILNTLYHQSVNNQREISELSGHSLGVVNKSIKELMNE
                     GYINEKCAVTPKALKEFKEKAPKNAVILAAGYGMRMVPINTETPKGLLEVNGEVLIER
                     TIRQLHEVGIYEIYVVVGFMKERYEYLIDDFGVELVVNEEYTTKNNLYSVKKVLNHLS
                     NTYIIPCDIWCDKNPYHHHELYSWYMVSDLIDDDSTVRVNRKMELVTIPKAAGGNAMI
                     GISYLLDDDAQIVKGRIEKLCSNSANDGAFWETALYDKDRMFITARVVHSWDVVEINT
                     YEQLREMDSDSNHLKTDAIQVICDALSVSADDIVDITVLKKGMTNRSFLFSCKGKKYI
                     MRIPGEGTDQLINRRNEAMVYNTIDGRHICDDIAYINPDNGYKITAFLENARVCDPEN
                     NDDVCKCMKRLREFHDMKLKVDHEFDIFGQLEFYESLWDGSPSAYRHYRQTKENVLSL
                     RPYIEAHVNEKVLTHIDAVPDNFLFVKNEDGNEDIRLIDWEYAGMQDPHVDIAMFCIY
                     SMYDREHVDKLIDAYFTEGCSAETRIKIYCYIAVCGLLWSNWCEYKRNLGVDFGEYSL
                     RQYRYAKDYYKVVQDEMQKLEDN"
     gene            6869..7585
                     /locus_tag="DHV81_01225"
     CDS             6869..7585
                     /locus_tag="DHV81_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase"
                     /protein_id="HCI93162.1"
                     /translation="MHEVKRAIIMAAGLGNRMRPVTLTTPKPLVRVNGKRMIDTVIEG
                     LHDNGITEIYIVAGYLKEQFYELENEYPGVTIIENPYYESCNNISSLYVARDHIEDAI
                     ILDGDQIIYNPEILSKEFERSGYNSVWTDDETDEWLQTVEDGIVTSCSRSGGSHGWQL
                     YSISRWTAADGRKLKKHLEMEFENKKNTQIYWDDVAMFCYPDEYQLGIYEMQKGDIIE
                     IDNLQELAEIDSSYKKYMED"
     gene            7590..9107
                     /locus_tag="DHV81_01230"
     CDS             7590..9107
                     /locus_tag="DHV81_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine transporter"
                     /protein_id="HCI93163.1"
                     /translation="MKEKKIDWIITLVPLAIIVALCILFFALPEQSNAILSQVRFFFG
                     DTFGIYYLIIGLGIFLISIFIAGSKYGDIVLGEPDEKPKYSFFSWGCMMFTCGLAADI
                     LFYSFSEWIMYATNPHIKELGSIQEWAGVFPLFHWSFIPWAFYLVLAVAFGFMLHVRK
                     RNRQKYSEACRPVLGKHTDGFAGRIIDLLAVFALLAGTATTFSVATPLMATIIGDVFH
                     ISLSRTAINIIILIITCIVYTYSLLHGFKGISILAKVCIYFFFGLLAIVLLFGGETRY
                     ILETGLSSLGTMVQNFIGLSTYTDPLRKTNFPQDWTIYYWAYWMVWCVAAPFFIGNIS
                     RGRTIRQTILGGYGFGVGSTLISFIILGNYSMGKQMSGAADFIAQYNKSGDLYELIVT
                     ILKTLPCAPLIMIIVLVTMIAFYATSFDSIALTVSCYSYRKLGEDEQPHKLIQLMWCI
                     LLILLPIALLFAESSMSNLQSVSIVAAFPIGIVIVMITISFLKDAKKYMGELKNK"
     gene            9397..10331
                     /locus_tag="DHV81_01235"
                     /pseudo
     CDS             9397..10331
                     /locus_tag="DHV81_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743424.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     assembly_gap    9957..10040
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10519..>11318
                     /locus_tag="DHV81_01240"
     CDS             10519..>11318
                     /locus_tag="DHV81_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCI93164.1"
                     /translation="MSEKRLQKRDEISDEYKWKLEDMYETDELWEAECEKASQIAEKL
                     SGFKGHLADSAATLLDFFKKQDELSYYLERIVVYANERSHQDTAVSKYQAYVSKAETL
                     TVQASGALAFASPEILQIDDKRLESFYSESNELMHYKRAIDEIMRQKEHTLSAAEENI
                     LAQCGEMAAAPENIFSMFNNADIKFPYITDVEGNKIRITHGNFIDFLSSKDRSLRKQV
                     FRGVYDSYKKWSNTVSMMYISKLKNDTFYARVRKYDSARAMYLSDGDIP"
BASE COUNT         3584 a         1838 c         2650 g         3162 t
ORIGIN      
        1 gattatcagg tgcttgctat ccgtgattac aagaatgaca caagaaaaga aatgacaaca
       61 ggaaaggttg agccgacagg tcttccttct tctaatgtac tttactacga acttaacgat
      121 aacgcttggt gctgtgtacg cccttctggt acagagccta agattaaatt ctatttcgga
      181 gttaaaggag tgtctcttga agattcacag gctaagcttg atgctttgtc agctgaggtt
      241 ctcggacagt ttgaataaag actatgatga aaatatacat ttgcccggat tgtggctgga
      301 tacggatggt ttcaagaagg aaagaggttg aatgcttcaa gtgtggcagg ccgcagatgc
      361 gtctgacgaa tctggagttt ggaaagtata ccaatatgtc ggagaaggag cgtcaggact
      421 acgcagacgc gtggctttac attcatagga aaaaggaaaa ataacatggc aaagaagaga
      481 agaagaaaaa agagaagaag tggacgtaag aagattatac tttttgtatt tgaaatcctg
      541 ttgctgttaa ttgttctgtt agtggtttgg gcttataata agacactcgg acaggttaaa
      601 tatgaggatg cgctgacagc tagtgaggcc ggaatcaatg aggatattga tgatgatgct
      661 cttgccaata tgcatggata tctcaatgta gcgttgtttg gtctggacaa cagaagtaat
      721 ggttcataca atgaggggca ttccgattgt atcatgatcg ctagtctgaa ttatgatact
      781 aaggaggttc agcttgtttc tgtctacaga gatacgtatc tgcctatagg caatggaaag
      841 tttgcaaagg caaatgcagc atatgctaat ggtggggcaa agagagcagt agctatgctt
      901 aattcaaatt tggatttgaa tattacgaag tatgtttgtg ttgactggaa ggctctcgta
      961 gatgctatag atgatatcgg aggtcttgat cttgagatta ctaaggcgga gatgaaggaa
     1021 atcaattatc tgattcctga ggttgattat actacaggat ataatactcc ttatcttgag
     1081 ggggatggta tgcagcatct tgatggaact caggctacat gctatgcacg tatcagaagc
     1141 acctctggtg atgatttctt aagggcatct cgtcagagaa ttgtgcttca ggctatgctt
     1201 gacaaggcta agcagtcaga tttaggctcg ctcacagaga tgtgtaaaga tatcatgagt
     1261 cagataagta caaactttac agctaccgag atattacagt atgcttctta tgttacaaag
     1321 tttcagatga aggagacaac aggcttccca tttgagctga ctacaatgaa tctttcatct
     1381 actggggata cagttattcc aattgattta gcacagaatg tttccaaact tcatcagttt
     1441 ctgtttaatg agactgatta tcagccaact gagacagttc aaagtgtcag tgacaagatt
     1501 gctaaaaaaa caggtgttac gtcaaagact tctgcatttg atctctcaca gtacaatgat
     1561 actgttggtt ctgatggaac cgaaggggca aagaaaaaga ataaggataa gcaggattca
     1621 ttaaagaaca gtcagtcgga gtctgaaaat aattctggaa cgaaatcatc atcagatgat
     1681 tcagatgagt aattacctga tggtatttat ttagctgtta taagcaataa gttttataaa
     1741 acaatattta tatgaacaat atatccgggg ccaatcaata gattggtccc acataattta
     1801 atatgattaa taatgtaata acctataact aatgaatagt taatggaaaa tcaatgatta
     1861 aataacaact aaattacaag aacaagggag aatttatgaa aacagaaatt aaagaaaaaa
     1921 tcacagcaac caatcagaaa tacttaaaga aattcttaac agaggaagcc ccggctaagc
     1981 ttgagagcca gcttgagcac atggactggt cataccttga tatgattcac aacaaggagc
     2041 agcagagagg tacattttca ccgcttgctg ctatggagct ttcagagata gaggcaaata
     2101 aggacaaata ccttgcaaca ggttttgatg ctgtcaagaa tggcaaggtt ggtgcaattc
     2161 ttcttgccgg tggacagggt acacgtcttg gctttgacaa ggctaagggc atgttcaata
     2221 tcggaaagac aaaggaactc tacatctttg agcagcttgt cgcaaacctc atgaaggtta
     2281 caaatcagac cggtacatgg gtgcctctgt atgttatgac aagcgagata aatgatagca
     2341 tgacaagaga gttctttgaa gagcacgact attttggata caacaaggac tatgtgaagt
     2401 ttttcgtgca ggagatggtt ccggcagttg attttgatgg aaatctgctc atgaaatctg
     2461 aggattcact tgccatgtca ccaaacggaa acggtggatg gttcaagtca ctcatcaatg
     2521 ccggtcttga caaggatttg aaggacaagg gtgttgagtg gctcaatgta tttgctgtgg
     2581 acaatgtgct tcagcagatt gcagatcctg tatttgtagg tgctacaatc gagtccggct
     2641 gtgtcagcgg ttctaaggtg gtcagaaagt gcgatccata cgagagagtc ggcgctatgt
     2701 gtcttgagaa tggcaagcct tcgattgttg agtattacga gcttactcct gagatggcag
     2761 aggcaaagaa cgagaatggt tcattgcagt atggctttgg tgttatctta aactatttat
     2821 tccgcgtgga caagctcatg gctatcgcag agaagagcct tccacttcat gtggtagaga
     2881 agaaggtgcc ttacattgat gagaatggta cagagcataa gcctgagact ccgaatgcat
     2941 acaagttcga gacactcatc ctcgatatgg tatacatgat ggacaatagc ctgccatttg
     3001 aggtagacag agagatggag tttgcacctg taaagaatgc gacaggtaca gattcggttg
     3061 aaacagcgag agctcttctt gagaagaacg gaattgaaat ttaatatatg atttatggta
     3121 actgttcatt agcagggatg tattgatatt ctgcaatttc tatttatcgc agactcatag
     3181 gtgcatctgc cgatgagcag ttatttttat aatcgcatag tggcctgcaa tggggaacat
     3241 agactataaa tagttaactg atttatgaaa aaataaataa tcccgtcaca aattaaacac
     3301 agattgacag tatacttcta aatataaacg aaaggagaaa ttattatgga taacaattat
     3361 ggaaatatga atgacacaaa taatacaaat aatactaaca gtacaaataa caatacaaac
     3421 aatgtaaatt tcacaatgtc gggtaatcct tacaatccgg atgagtcaca gaaggctgca
     3481 cagactaata atacatcagc tgaaagctat tcacagagct acaatccata ttcgcaggct
     3541 tctgcagcag gacagccaaa tacaaaccag tcatatgcaa atcagtcata tgcaaaccag
     3601 ccttattcta atcagtcgta taattcaggc acaaataata atgcatatgg cggcgcatca
     3661 gccggttcga cagacaactc atatagccgg atgaatatga atggtcaggc ataccagcag
     3721 actgcaaagc ctaagaaaca gagaacacct agaaagcctg gtgcatttgg accatttgca
     3781 gcaaagacag tagcggcagc tcttatcttc ggactcgtcg gaggcggcgt tttcacagga
     3841 gtaagctata ttggcacgag agctatttca aattcttcta ctgcaagtgc aaagcttacc
     3901 actacaacat ccggtgggac taagcagaca tcctcaggga atgcaaagga tctgacagat
     3961 gtatcatcag ttgtcgatga ggtaatgcct agcatcgttg ctatcacaaa tacaggaaca
     4021 gtcacctaca acagcttttt cggaaagcag tcacagcagt ctcagagctg tggctcaggt
     4081 attattgtga gtgaggatga tgattatctc tacattgcta caaacaacca tgttgtggca
     4141 gattcagagg agcttacagt acagtttgat gatgattctg tggtaaaggc ggaaatcaga
     4201 ggaacggatc ctgacgatga tcttgcagtt gtacgtgtaa agaaagcaga ccttggaaaa
     4261 gatacttact caaatatcaa gattgctaca atcggagatt ctgaaagtgt agctgttgga
     4321 agtcctgcaa ttgctatcgg taatgcactt ggatacggac agtctgtcac aacaggtata
     4381 gtaagtgcgc ttaacagaac tgttactaca caggattcac agacaggtga gactgttacc
     4441 aacaacaagc ttatccagac agatgcagca ataaaccctg gaaacagtgg tggagcactc
     4501 ttgaatgaaa atggcgaggt tatcggtata aactctgtaa aatattcttc aactgaagtt
     4561 gagggtatcg gatatgcaat tcctatgtcg gttgcaaagc ctattattga gagccttatc
     4621 caggatggaa aatatactaa tgagaatcag gcataccttg gtatcaaggg tggcgatgtt
     4681 tcatctgaga tggtagccta tggcttccca cagggtgtgt atgtttcatc tgtttcagct
     4741 ggttcaggtg ccgcaaatgc cggcttacag gagggagata tcatcactgc tgttgacagc
     4801 acaaagattt cttctatgac agagctgcag tcagcgttaa agagctacaa ggccggagat
     4861 aaggttactc ttactgttgc ccgtcagagt ggcagacagt acgaagagag caaggttgaa
     4921 gtaaccttaa gctctgcaaa ggatattcag cagtaaataa ttcatttata tttatgagta
     4981 caaaaacgcg ccagtgtggc gcgttttttg ttatgaaaaa tatagttaag ggttgatttt
     5041 tttaataaaa aggtgtagta ttatgaatgt tcataaaaac ataaggagag gcgaacatga
     5101 acaaacaaga gaaagatata ctgaacacgt tatatcatca atcagttaac aatcaaagag
     5161 agatatcaga gttatccggt cattctctgg gagttgtaaa caaatcaata aaagaattga
     5221 tgaatgaagg ttatataaat gaaaaatgtg ctgttacgcc caaggcactt aaagaattca
     5281 aagaaaaggc tccgaagaat gcagtaattc tggcagctgg ttatggaatg cgaatggtgc
     5341 ctataaatac cgagactcca aaaggccttt tagaggtaaa tggggaagtt cttatagagc
     5401 ggactatcag acagcttcat gaagtgggaa tttatgagat atatgtagta gtcggcttca
     5461 tgaaggagag atatgagtat ctcatcgatg attttggtgt agagctcgtt gtcaatgaag
     5521 agtatacgac taaaaataat ctttattcag taaagaaggt tttgaatcat ttatcaaata
     5581 catatataat accgtgtgat atatggtgtg acaagaatcc gtaccatcac catgaattat
     5641 attcctggta tatggtcagt gatttgattg atgatgacag cacggtgcgt gttaacagaa
     5701 aaatggaact ggttaccata ccgaaggctg ccggcggcaa tgctatgata ggaattagct
     5761 atctgcttga cgatgatgca caaattgtca aaggcagaat tgaaaaactg tgtagtaatt
     5821 cggctaatga tggtgccttc tgggagactg cactgtatga taaggacaga atgtttatta
     5881 ctgccagggt agtgcattcg tgggatgtgg ttgaaattaa cacatatgag cagctcagag
     5941 agatggacag cgattcaaat catctgaaaa ctgatgcaat acaggttatc tgcgatgctt
     6001 tatctgtatc agcagatgat attgtagata ttacggtatt gaaaaagggc atgacaaacc
     6061 gctcgttcct gttctcatgt aaagggaaaa aatatatcat gagaattccg ggagagggaa
     6121 cagatcagct gataaacaga cgtaatgaag caatggttta taatactatt gatggcagac
     6181 atatatgtga tgatatcgct tatataaatc cggataatgg atataaaatc actgcgtttt
     6241 tagaaaatgc gagagtatgt gatccggaaa ataatgatga tgtttgtaaa tgcatgaagc
     6301 gtctcagaga atttcatgat atgaagctta aagtagatca tgaatttgat atattcggtc
     6361 agcttgagtt ttatgagagc ctgtgggatg gttcgccttc tgcatacaga cattacaggc
     6421 agactaagga aaatgtcctt tcactcagac cgtatataga agctcatgta aatgaaaagg
     6481 ttcttacgca tattgatgct gttccggata atttcctttt tgttaagaat gaagatggaa
     6541 atgaggacat cagacttatt gactgggaat atgcaggaat gcaggatccg cacgtagata
     6601 tagccatgtt ctgcatatat tccatgtatg acagggagca tgtggataag ttaattgatg
     6661 catatttcac agaagggtgt tcagctgaaa cccgaataaa gatttattgt tatattgcag
     6721 tatgcggact tttatggagt aactggtgtg agtataagag aaatctgggc gttgattttg
     6781 gtgaatattc acttagacag tacagatatg caaaggatta ttacaaagta gtacaggatg
     6841 aaatgcagaa attggaggat aattaagaat gcatgaagta aaacgcgcga ttattatggc
     6901 ggcaggactt ggtaatcgta tgagaccggt aacgcttact actccgaagc cattggtaag
     6961 ggtgaatgga aagcgtatga tagatacggt tattgaaggc ttacatgata atggaataac
     7021 ggagatttat attgttgccg gatatttaaa ggaacagttt tatgaattag agaatgaata
     7081 tccgggagtt actataattg aaaatcctta ttatgaaagc tgtaataata tttcatcact
     7141 atatgttgca agagatcata ttgaggatgc aattattctt gatggtgacc agattatata
     7201 taatcctgaa attctatcaa aagaattcga acgttcgggt tataacagtg tatggactga
     7261 tgatgaaaca gatgagtggc ttcagacagt agaagatgga attgtaacat cctgcagcag
     7321 gagtggcgga agtcatggat ggcagcttta tagcatctcc agatggactg cggcagatgg
     7381 gcgcaaactg aaaaaacatc ttgaaatgga atttgagaac aaaaaaaata ctcagatata
     7441 ctgggatgac gttgcaatgt tctgctatcc ggatgaatat cagcttggca tatatgagat
     7501 gcaaaaaggc gatattattg agattgataa tttgcaggag cttgctgaga tagatagcag
     7561 ctataagaaa tatatggagg attagtatta tgaaagagaa aaaaattgat tggattatca
     7621 cacttgtgcc attagcaata attgtggcat tgtgtattct attttttgca ttacctgagc
     7681 agtcaaatgc catactcagt caggtgagat ttttctttgg agatacgttt ggcatttatt
     7741 acctgattat aggtttagga atatttttga tatctatatt tatagctggt tcgaagtatg
     7801 gagatattgt tcttggagag ccggatgaaa aaccaaagta ctcattcttt tcatggggat
     7861 gtatgatgtt cacatgtggt ctggcagcag atattctttt ctactcattt tctgagtgga
     7921 ttatgtatgc aaccaatccc cacattaagg agcttggaag catacaggaa tgggccggtg
     7981 tatttcctct gtttcattgg agctttattc catgggcatt ttatcttgtt ttagctgtag
     8041 catttggttt tatgcttcat gtaaggaaaa gaaaccgtca gaaatattcg gaagcatgca
     8101 gacctgttct tggaaaacac acagatggtt ttgcaggacg tattatagac ctgttagctg
     8161 tgtttgcact tttagcagga acagcaacca catttagtgt tgcaacacct cttatggcaa
     8221 caattattgg agatgtgttc catatatcgc ttagcagaac agcaatcaat attattatcc
     8281 ttataattac atgtatagtt tatacatatt cattattaca tggatttaag ggaataagca
     8341 ttttggcaaa agtctgcatc tatttcttct ttggactgct tgctattgta ctgctgtttg
     8401 gcggtgaaac aagatatatt cttgagaccg gattgtcatc tcttggcact atggtgcaga
     8461 attttatagg attatctacg tataccgatc cattacgtaa aaccaacttc cctcaggact
     8521 ggaccatata ttactgggct tactggatgg tatggtgtgt agcagctcct ttctttattg
     8581 gaaatatatc aagaggcaga actatcagac agacaattct tggaggatat ggatttggtg
     8641 ttggctctac gctcatcagc tttatcatct taggcaacta ttcgatggga aaacagatgt
     8701 caggagcagc agattttatt gcgcagtaca acaagagtgg tgatttatat gagttgatag
     8761 ttactattct taagacattg ccatgtgcac ctttgataat gataatagtt ttagtgacaa
     8821 tgattgcttt ttatgctaca tcatttgatt ctattgcttt aactgtatca tgttacagct
     8881 acaggaaatt aggtgaggat gagcagccgc acaaactcat tcagctgatg tggtgcatac
     8941 tgcttatact tttaccaatt gctctgttat ttgctgagag ctcaatgagt aacctgcagt
     9001 ctgtaagtat agttgctgca tttccgatag gaatagttat tgtgatgata acaatcagtt
     9061 ttcttaaaga tgcaaaaaaa tatatgggtg aattaaagaa taaatagaaa taaaatagtt
     9121 caatatatgt gtaagaatgc ttcgctacat ggtagtagga gcattctttt ttcgcttacg
     9181 tcccttacgc tgtggcggaa atgttgttca aatcggttca aagtactaga caaattccct
     9241 aaaatattag ataatgtata taaataattt tacttttgca ccgcatagga attcttcaaa
     9301 attgatggtt tatatgtaga tttataatag tatacacatc ggtttatgat tatgagatat
     9361 aatacatatt gagcggatgc agcataggag gtcggcatga agaagaaaaa aatagttatt
     9421 gcactcggac atgatgctct tggaacaact cttccggagc agcacgaggc tacaaagcat
     9481 acagcaaagg cagttgcaga ttttatcagg gacgattatg aggttgttat cacacacagc
     9541 aacggaccgc aggtgggcat gattcacacc gctatgtctg agttttgcag gatttatcct
     9601 gagtatacag ctacacctac agcagtctgc tctgcaatga gccagggcta tataggatat
     9661 gatttacaga atgctatccg tacagagctt cttggcagag gaatttacaa gaccgtttct
     9721 acaatactta cacaggtggt tgttgaccca tatgatgagg ctttttatca tccgacaaag
     9781 atcatcggcc gtgttatgac agaggctgag gctgaggaag aagagaagaa gggcaaccat
     9841 gtgacacagg ttgagggagg ctacagaaga gttgtggcag ctccaaagcc tgttgatata
     9901 gtggaaaagg atgctattaa ggctcttgtt gatgcggatc aggttgttat cgcatgnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn ggaaagcttg cagatactct tgatgctgac agacttgtta
    10081 tacttacaag cgttgacaag gtctgcctga actatggcaa ggaggatgag aagccgcttg
    10141 ataccatgac tgtcgatgag gcaaagaagt accttgcagc aggcgagttt gatgaaggta
    10201 ccatggctcc aaagattgag gcggctatcg attttatcgg tgactctgcc atccgttcag
    10261 tgcttatcac aaagctcaat aaagagggca agacagtttc aggtggtatg ggcactttaa
    10321 ttacaaagta gagcttaatt gttataattc acatatatcc cacacgtcta tatgcatttt
    10381 gcagagcaga tgtgtgggta gttttataaa aacatcgtgc ggttttgcga atgtgcgtgg
    10441 gggttctgaa gagttgaaaa caaggcgact attcagaacc cccgagaaca agaaaagtaa
    10501 gatagaaggt aacacaaaat gagtgagaaa aggcttcaaa agagagatga aatctcagat
    10561 gagtacaaat ggaagcttga ggatatgtat gagacagatg agctgtggga ggctgagtgt
    10621 gaaaaagcgt cacagattgc agaaaagcta tctggattta agggacatct ggccgacagt
    10681 gcagcaacgc tgcttgattt ttttaagaag caggatgagc tgtcttatta tttggagcgt
    10741 atagtggttt atgcgaatga acgctcgcat caggacactg ccgtgtcgaa atatcaggca
    10801 tatgtgtcaa aggcagagac gctgactgtt caggcatcag gtgcgctggc atttgcgagt
    10861 ccggagattt tgcagataga tgataaaagg cttgagagct tttacagtga gagcaatgag
    10921 cttatgcact ataagcgtgc gattgatgag ataatgcgtc agaaagagca tactttatcg
    10981 gctgcagagg agaatatcct ggcgcagtgc ggtgagatgg ctgcagcacc ggagaatata
    11041 ttcagcatgt tcaacaacgc ggatatcaaa ttcccatata tcactgatgt cgagggaaat
    11101 aaaatacgta tcacgcacgg caatttcata gactttttaa gcagcaagga cagaagcctg
    11161 cgcaagcagg tgttccgagg agtgtatgat tcgtacaaga agtggagtaa tactgtttca
    11221 atgatgtaca tcagcaagct gaaaaatgac actttttatg caagggtaag aaaatatgac
    11281 agcgcaaggg ccatgtattt atccgatggc gatattcc
//
LOCUS       DPUF01000035           12750 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3222, whole
            genome shotgun sequence.
ACCESSION   DPUF01000035 DPUF01000000
VERSION     DPUF01000035.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12750)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3222"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="DHV81_01245"
     CDS             complement(<1..326)
                     /locus_tag="DHV81_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HCI93165.1"
                     /translation="MKYSKRYIAFTFILALIFVSNFYIYAKDSSTLGAFRGAQIDNTI
                     WSPLVAADVNGTTIRLMIENKEYTSEDEHVYMDENRNIMVPVSMLRDALNSSAHVYNQ
                     NELLVE"
     gene            complement(335..1486)
                     /locus_tag="DHV81_01250"
     CDS             complement(335..1486)
                     /locus_tag="DHV81_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCI93166.1"
                     /translation="MNRFELVVPCHFGLEAVVKREIYDLGYEITRVEDGRVSFEGDAE
                     AICRANIFLRGAERVLLQVGRFKAATFDELFENVKALPWENYIPKDGRFWVKKASSIK
                     SKLFSPSDIQSIVKKAMVERMKRSYHIDWFPEDGAQYPVRVFLYKDEVMVTLDTSGDS
                     LHKRGYRLATSKAPLTETLAASLIMLTPWHADRILVDPFCGSGTFPIEAAMMAANIAP
                     GMNREFTAESWTNIIPRKLWYEAVQEAEDMIDKDVQVDIQGYDIDLDVVAAARENAKR
                     AGVEHMIHFQQRAVADLHHPKKYGFIISNPPYGERLEEKSALPALYSQIGEAYKRLDA
                     WSMYLITSYEDTERYIGRKADKNRKIYNGMIKTYFYQFMGPKPPRRKKD"
     gene            complement(1570..1887)
                     /locus_tag="DHV81_01255"
     CDS             complement(1570..1887)
                     /locus_tag="DHV81_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCI93167.1"
                     /translation="MKFKIVYADELYKVQKEMNALLIDIRTREEYDRSHWKGALSMPM
                     DITDSYESELDVSQSVIFYCTHGGSSMKLARYLGNRGFDTATVIGGYNAMCGYQKKLQ
                     KYG"
     gene            complement(2201..4228)
                     /locus_tag="DHV81_01260"
     CDS             complement(2201..4228)
                     /locus_tag="DHV81_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCI93168.1"
                     /translation="MSNRFDDQNNIEMDIKTAFASWGKFVFKCFPQEGRLRSFLSEDS
                     LDDSEEKNVDIINNPFIDSMVDRDNLLKAVEQIAEGEKFATVVLHAKEGTDTYRISFK
                     SICFDDDGRTVEAIGLAEQLDERLNESEMVNALGSNFNSIYYVDVDNNKMYPYRMNPS
                     VHAMFGEVLESKPGYQEMMEQYVNAIVLEEDKLHMLLETSINNLRRQFLIKDTYQHDY
                     RIVRDGQVQYCRAKFVNVSGVGELHKMIAGFSDISPEKQRELERIAYVDAVTGGNNYE
                     SFKKKLRDRNVSGYLISMDIHSFKIVNSICGVAKGDEALRWISENIRGVVGYNDISGH
                     INADRFVIFFADDKINKVIRKIETINLQLIKVSESLKIPKIQPYFGVTKWEPGKKVEE
                     AYGEANFAKNRIKERKDVLYQIYSQADTERIVEEKQMEDNFYRDLNDNHFEIWYQPKY
                     APKTNKLVGAEGLVRWRRENNEIIPPSKFIPLFERNGMIKALDEYVFREVCTHQRRWL
                     DEGKKIVPISINLSRASLYFESVVKRYHDIARRIGIYTHLVPIEITESAAVDNDEIKS
                     IADKFHGNGFPLLVDDFGSGYSSLATLNMKCFDTLKIDKSLIDYIGDSNGERLLVHTI
                     ALAKELGICVTAEGVESQEQVEFLKNIDCDSIQGFYYSKPLPLAEYEMLLD"
     gene            complement(4362..5648)
                     /locus_tag="DHV81_01265"
     CDS             complement(4362..5648)
                     /locus_tag="DHV81_01265"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741778.1"
                     /note="catalyzes the formation of L-methionine and acetate
                     from O-acetyl-L-homoserine and methanethiol; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HCI93169.1"
                     /translation="MKEYSIGTKCVQAGYAAGNGEPRQIPIIQSTTFKYDTSEDMGKL
                     FDLEAEGYFYSRLQNPTCDTVARKIAAMEGGTAAMLTSSGQAANFYAMFNICECGDHI
                     VASSSIYGGTFNLIAVTMAKMGITATFVSPDATEEELNAAFRPNTKLMFGETIANPAL
                     TVLDIELFAKVAHEHGVPLIVDNTFPTPVNCRPIEWGADIVTHSTTKYMDGHGATLGG
                     AIVDSGKFDWMAHADKYPGLCTPDDSYHGITYAEKFGKEGAFITKCTAQLMRDFGSMQ
                     SPQNAFMLNLGLESLHVRMPKHVENGQAVAEFLENHPKVAYVNYSGLPSNKYYERAQK
                     YLPNGGCGVVSFGLKGGREAASAFMKALRLGAIETHVADARTCCLNPATSTHRQMNDE
                     QLKEAGVPAELIRISLGLEDKVDLIADISNALDAIK"
     gene            complement(5695..6735)
                     /locus_tag="DHV81_01270"
     CDS             complement(5695..6735)
                     /locus_tag="DHV81_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Archaeal ATPase"
                     /protein_id="HCI93170.1"
                     /translation="MQNPFTLSFGKKPLQYISRISQTNQIVETFRSENPVNQLFMITG
                     VRGSGKTVMMTNIVSEIKKDEEWLTVELNPTRDMLQSLAAKIYSMPQMNKIFVNAKLD
                     FSAFGLGVSIENAAPVTDIENVLELMLENIKKHNKRLLISVDEVTNCEFVKVFVSSFQ
                     IFLRQDYPIFLLMTGLFENIYDLQNDKALTFLYRAPKIMLEPLSFTAVRKHYMDIFEL
                     DQREADKMAALTKGYPFAFQVLGYLYWENRDDHTIEDILPEYDQCLDEYVYSKIWSEL
                     SELDKKILLEISVSGEEKVKEIREKLDMNSGLFSVYRDRLKRKGVIDTSEYGKITLTL
                     PRFEEFVKTRQM"
     gene            complement(6853..8359)
                     /gene="cobA"
                     /locus_tag="DHV81_01275"
                     /pseudo
     CDS             complement(6853..8359)
                     /gene="cobA"
                     /locus_tag="DHV81_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568992.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
     assembly_gap    7513..7526
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8421..9437)
                     /locus_tag="DHV81_01280"
     CDS             complement(8421..9437)
                     /locus_tag="DHV81_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCI93171.1"
                     /translation="MRYLIGTRGSKLALVQTDYVIDKLKRAYPDDDFKAVIIKTTGDL
                     EQKKALDKIGSKGIFVKEIEDELLSGKIHMAVHSMKDMPDEPAAGLTFAKAWKREDPR
                     DVLVLKNAASLDELPHGAVIGTGSKRRKYQLLKLRPDLKVVGIRGNIDTRLRKLYDGE
                     IIYEEIADNTVNNNEIANEKQNKYNEYRLDGIVLAAAGIKRIGRDSEITQYFSEDDMI
                     PAPAQGTLALELRADNAKLMAKLDALSDEKTNLCAGIEREFLKRIGGNCHLPVAAYCD
                     IVSEKDRGNDSNKNNRLKLLAMFGSEDGKKLARTEQVWDITSDRNNIVERAVHDIRYK
                     LEID"
     gene            complement(9522..10184)
                     /locus_tag="DHV81_01285"
     CDS             complement(9522..10184)
                     /locus_tag="DHV81_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 oxidase"
                     /protein_id="HCI93172.1"
                     /translation="MAYFPMFVDMTERECLIVGGGNVAYRKVIVMLDFGAKVTVVAED
                     ICDELRKLTIDDIASEDKTGSYTANKENNQTDSDAADRITFIKRRFERKDCDGMEMVI
                     AATDDNALNHEIAEYCKANGIMVNAVDQKADCSFIFPSYIKEKNLVAAFSSGGNSPVL
                     TQYLKGKEQEILTPFLGELNEYMGQIREKVIAQYDTQAERKRVFKEILCAAIDNGRIP
                     EI"
     assembly_gap    10222..10311
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10312..10883)
                     /locus_tag="DHV81_01290"
                     /pseudo
     CDS             complement(10312..10883)
                     /locus_tag="DHV81_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568995.1"
                     /note="too many ambiguous residues; incomplete; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     assembly_gap    10689..10722
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11016..11447)
                     /locus_tag="DHV81_01295"
     CDS             complement(11016..11447)
                     /locus_tag="DHV81_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93173.1"
                     /translation="MSIIRMKYPFLDEIAKYAKEHLPEEACGLIAGSEDENGRLITKV
                     YYLTNTDHAEDHFTMDPKEQLAAIKDMRANGLKPLGNWHSHPSSPSRPSDEDIKLAYD
                     PNASYMIMSLMAENPVINSFHIEGGQVTKEDLRIYSDEYYF"
     gene            complement(11472..12311)
                     /locus_tag="DHV81_01300"
     CDS             complement(11472..12311)
                     /locus_tag="DHV81_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCI93174.1"
                     /translation="MAMTDEQIERYSRHIILNEVGVKGQKKLLKGKVLIIGAGGLGAP
                     AAMYLAAAGVGTIGIVDADEVDLSNLQRQIIHGTADVGKAKVKSAKETMNAMNPDVQV
                     NTYRMFVDASNIRELIRDYDFIIDGTDNFPAKFLINDACVLEKKPFSHAGIIRFKGQL
                     MTYVPGEGPCYRCVFKNPPPKDAVPTCKQAGVIGAMGGVIGSLQAMEAIKYLIGVGEL
                     LTGYLLTYDALTMEFHKIKLPKDTHNCAVCGDHPTILEPIDYEQEVCEETAGRFDAPR
                     TQE"
     gene            complement(12311..12517)
                     /gene="thiS"
                     /locus_tag="DHV81_01305"
     CDS             complement(12311..12517)
                     /gene="thiS"
                     /locus_tag="DHV81_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiS"
                     /protein_id="HCI93175.1"
                     /translation="MTITVAGEKKEYKDGLTLPELIELENVDMPEYVTVSINEEFVAT
                     EDKPKTVLKDGDNVEFLYFMGGGC"
     gene            complement(12601..>12750)
                     /locus_tag="DHV81_01310"
     CDS             complement(12601..>12750)
                     /locus_tag="DHV81_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HCI93176.1"
                     /translation="NTNFDGELADEYEVTANPTLLFVKDAEVLDRKVGAQKPADLKAW
                     VEELL"
BASE COUNT         3213 a         3137 c         2141 g         4119 t
ORIGIN      
        1 ttttccacca gaagctcatt ttggttgtat acatgggcac tgctgtttag tgcatcgcga
       61 agcatagaca cgggaaccat tatattcctg ttctcatcca tatacacatg ctcatcctcg
      121 ctcgtatact ccttattttc tatcataagc ctgatggtgg tgccatttac atcagcagcc
      181 acaagcggac tccatatagt attatctatc tgtgctcctc tgaacgcgcc aagtgtagag
      241 ctgtcctttg catatatgta aaaattagac acaaagataa gtgccaagat gaatgtaaat
      301 gcaatatatc tctttgaata tttcatatac cctgctaatc ctttttcctt ctcggaggct
      361 taggccccat aaactgataa aaatatgtct ttatcatacc attgtagatt ttcctgtttt
      421 tgtcagcttt tctgccgata tagcgctctg tatcttcata gctcgtaatc agatacatcg
      481 accatgcatc aagcctctta taagcttctc caatctgtga atacagtgcc gggagcgctg
      541 atttctcctc gagtcgctca ccgtatggag gatttgagat aataaatcca tacttcttcg
      601 ggtgatgcag gtcagccaca gccctctgct ggaaatgaat catgtgctcc actccggctc
      661 tcttggcatt ttccctggct gcagccacca catcaaggtc aatgtcatag ccctggatgt
      721 ccacctgcac atccttatct atcatatcct ctgcctcctg cacagcctca taccacagct
      781 ttcttggtat gatattggtc cagctctcag ctgtaaactc cctgttcata ccgggtgcta
      841 tgtttgctgc catcatggct gcctctatcg gaaatgtacc actgccgcaa aacggatcca
      901 caagtattct gtctgcatgc catggtgtaa gcatgatgag cgatgctgca agtgtctcag
      961 tcagcggcgc cttgctcgtt gccagacggt atcctctctt gtgaagggaa tcacccgatg
     1021 tatcaagtgt caccatcacc tcatccttgt acagaaaaac ccttactgga tactgtgctc
     1081 cgtcctccgg gaaccagtca atgtgataag acctcttcat tctctcaacc atagcctttt
     1141 tcacgataga ctgtatatca gatggtgaaa aaagcttact ctttatggaa gaagcctttt
     1201 tcacccagaa tctgccgtcc ttgggaatat aattttccca tgggagagcc tttacatttt
     1261 caaaaagctc atcaaatgtg gcagccttaa agcgtcctac ctgaagtaat actctctctg
     1321 cgcctcttaa gaatatattc gctctgcata tagcctcagc atcgccctca aacgataccc
     1381 tgccgtcctc cacacgtgtt atctcatatc ccagatcata tatctccctc tttacgactg
     1441 cctcaaggcc aaaatgacat ggcacaacaa gctcaaatct attcatatat tgtgtaactg
     1501 cctttctata attgtacttt acattgttat ctaatattgt tgcataaatt tcaatttttt
     1561 taaatagttt cagccatatt tctgcaactt tttttgatat ccgcacatag cattgtatcc
     1621 acctatgact gtcgctgtat caaaaccgcg atttcccaga tatctggcca gcttcatgct
     1681 gcttccacca tgcgtgcagt aaaaaatcac actctgtgac acatcgagtt cactctcata
     1741 tgaatccgtg atatccatag gcatgctgag tgcgcctttc cagtgcgacc tgtcgtattc
     1801 ctctcttgtt cttatatcta taagcagtgc attcatctct ttttgcacct tataaagctc
     1861 atccgcataa actattttaa atttcataca tccatacgcc aaaatacgaa tttacattat
     1921 gatattcatt ttattatgag atgtgaactg cgataaatca ccaaaatcat tatagctggc
     1981 actttagtac tttaactctt taataaatta acccgtttta ttaagattat attattttgt
     2041 gtttcaaagt attgcttttt aattactgtt attttataat gtttccgaag agagaaatct
     2101 tttcaatgtt atacctctta ttaattattt atactcccct caaaagagcc ggtcacgtga
     2161 ccggctcttt tatactgctt atattttttt atatttatta tcaatccaaa agcatctcat
     2221 actcggctaa cggcagtggc tttgaataat aaaagccctg tatactgtcg cagtctatat
     2281 tcttcaaaaa ctctacctgc tcctgactct caacaccctc ggctgtcacg cagattccca
     2341 gctccttggc cagtgctatc gtgtgcacaa gcagcctctc accatttgaa tctcctatat
     2401 agtcaatcag actcttgtcg attttaagtg tatcaaagca cttcatattg agggtagcaa
     2461 gtgatgaata gcctgagcca aaatcatcca ccaaaagcgg aaatccattg ccatggaatt
     2521 tatccgcaat actcttgatt tcatcattat cgaccgctgc actctctgta atctctatag
     2581 gcacaagatg tgtgtatatg ccgattcttc ttgctatatc atgatatctt ttgaccacag
     2641 actcaaagta caggctcgct ctggacaggt tgattgagat aggaactatt tttttccctt
     2701 catcaagcca tcttctctga tgtgtacaca cctcgcggaa tacatactca tcaagcgcct
     2761 taatcatgcc gtttctttca aaaagcggta taaatttgga tggcggaata atctcattat
     2821 tttctcttct ccacctgaca agtccctctg caccgaccag cttgttggtc tttggagcat
     2881 acttgggctg ataccatatc tcaaaatgat tgtcatttaa atccctgtag aagttatcct
     2941 ccatctgctt ttcttcaaca atcctttctg tatcggcctg actgtatatc tgatagagca
     3001 cgtccttccg ctccttgatc cgattcttgg caaagttggc ctcgccgtat gcttcctcaa
     3061 cctttttgcc cggctcccat tttgtcacac caaagtatgg ctgaatcttt ggaatcttta
     3121 aggattctga aaccttaata agctgtaaat tgatggtctc tatctttctg ataaccttat
     3181 ttatcttgtc atccgcaaag aaaataacaa atctgtctgc atttatatga cctgatatgt
     3241 cattatatcc tacaacaccg cgtatatttt cagaaatcca tctaagcgcc tcatcgccct
     3301 ttgccacacc acagatggag ttcactatct tgaaggagtg aatatccatg gaaatcagat
     3361 atcctgatac gtttctgtca cgcagcttct ttttaaaact ctcgtaattg ttgcctccgg
     3421 tcactgcatc cacatacgct atacgctcaa gctctctttg cttctccggg cttatatccg
     3481 aaaagcccgc tatcatcttg tgcagctctc ccacgccaga cacattgaca aacttggctc
     3541 tgcaatactg tacctgtcca tccctcacga tgcggtaatc atgctgatag gtatccttta
     3601 tcaaaaactg cctgcgcaga ttattaattg atgtctctaa aagcatgtga agcttatcct
     3661 cctctaagac gatagcattt acatactgtt ccatcatctc ttgatagccc ggttttgact
     3721 caagcacttc tccaaacata gcatggacag aaggattcat cctgtatgga tacatcttat
     3781 tgttgtctac atctacatag tagatactgt tgaagttgct tcctaaagca ttcaccatct
     3841 cactctcgtt aagacgctca tcaagctgct cagcaagccc tattgcctca actgttctgc
     3901 catcatcatc aaagcaaatc gacttgaatg atatcctgta ggtatctgtt ccttccttgg
     3961 catgcagtac tacagtagca aatttttctc cctctgcaat ctgttcaaca gccttcagca
     4021 gattatctct gtcaaccatg ctgtctataa acggattgtt tataatatcc acatttttct
     4081 cttctgagtc atctaatgaa tcctctgata aaaaagacct caaacggcct tcctgcggaa
     4141 agcacttaaa aacaaacttt ccccaggatg cgaatgcggt ctttatatcc atctctatat
     4201 tgttttgatc atcaaatcta ttactcataa taattcccac aaaaataaaa taattattaa
     4261 aactctttca tacctatcac tactatatca ttagtaagtc atttcgtaaa gttttgtttt
     4321 tactaatttt gttctttttg tgtattttat gaatatatat tttattttat agcgtcgagt
     4381 gcgtttgaga tatctgcaat caaatccacc ttgtcctcaa gacctaagct tattctgata
     4441 agctctgccg gcactcctgc ttccttaagc tgctcatcat tcatctgacg atgcgtgctt
     4501 gttgccggat ttaagcagca ggttctcgca tctgccacat gtgtctcgat tgctccaagc
     4561 cttaatgcct tcataaatgc agacgctgcc tctctgccgc cctttaagcc aaatgatacg
     4621 actccgcagc ctccgtttgg aagatacttc tgtgctctct catagtattt gtttgaagga
     4681 agccctgagt agtttacata ggcaaccttt ggatgatttt ccaaaaattc agccactgcc
     4741 tgtccatttt ccacatgctt tggcatacgc acatgaagtg actcaaggcc aagattcagc
     4801 ataaatgcat tctgtggtga ctgcatagaa ccaaaatcgc gcataagctg tgctgtacat
     4861 ttggtaatga acgcaccttc cttgccaaat ttctctgcat atgtgatgcc atgatagctg
     4921 tcgtcaggtg tgcacagtcc cggatattta tctgcatgtg ccatccagtc gaatttgccg
     4981 ctgtctacga ttgctccgcc gagtgtggct ccgtgtccgt ccatgtactt tgtggtagag
     5041 tgtgtgacaa tatctgcgcc ccactcaatc ggtctgcagt ttacaggtgt cgggaaggta
     5101 ttgtccacta taagcggtac tccgtgctcg tgtgccacct ttgcaaaaag ctctatatca
     5161 agcaccgtaa gcgcaggatt ggcaatagtc tcaccaaaca taagctttgt gtttggtctg
     5221 aatgctgcat tcagctcctc ctctgtagca tcaggagata cgaatgtagc tgtaataccc
     5281 atcttcgcca tagttaccgc gatgaggttg aaggtgcctc catagatgga tgatgatgcc
     5341 acgatatggt ctccgcactc acatatgttg aacatggcat agaaattggc tgcctgtcct
     5401 gatgatgtga gcatggcagc agtgccaccc tccatagctg cgatttttct tgctaccgta
     5461 tcacaggttg gattctgcag tctgctgtag aaatagccct ctgcctcaag gtcaaaaagc
     5521 tttcccatgt cctcgcttgt gtcatatttg aatgtagttg actgaataat cggaatctgt
     5581 ctcggctctc cgtttccggc tgcatagcct gcctgtacac actttgtacc tatactgtat
     5641 tctttcattg tgcttctcct ttattatgtt aatgtttcag atggatatag tatattacat
     5701 ttggcgcgtt ttgacaaatt cttcaaatcg tggaagtgtg agagtaattt taccgtactc
     5761 agaggtatct ataaccccct ttcttttgag ccggtctcta tataccgaga ataatcctga
     5821 attcatatcc agtttttctc tgatttcttt taccttttct tcaccactta ctgatatttc
     5881 caacaatatt tttttatcaa gctctgacag ctccgaccag attttactgt acacatactc
     5941 atcaaggcac tgatcatatt ccggtaaaat atcttctatc gtgtgatcat ccctgttttc
     6001 ccagtaaaga tatcccaata cctggaatgc aaaaggatat ccctttgtaa gagctgccat
     6061 tttgtctgcc tctctttgat ccagttcaaa aatatccata tagtgtttcc tgactgctgt
     6121 aaagctaagt ggctcaagca ttatcttagg cgccctgtac aaaaatgtaa gtgccttgtc
     6181 gttctgcaga tcatatatat tttcaaacag ccccgtcata agcagaaaaa taggataatc
     6241 ctgtctcaga aatatctgaa atgaactgac aaatactttt acaaattcac agttcgtaac
     6301 ctcatcaact gaaatgagta accgcttatt atgtttcttt atattttcaa gcatcaattc
     6361 cagcacattt tctatatccg taaccggtgc tgcattttct atggaaacgc ccagtccaaa
     6421 cgctgaaaaa tccagttttg cattgacaaa tattttattc atctgaggca tactgtaaat
     6481 cttggctgca aggctctgaa gcatatctct cgtagggtta agctcaactg tcagccattc
     6541 ctcatccttc tttatctctg atactatatt ggtcatcata accgttttgc ccgatcccct
     6601 taccccggtt atcataaaaa gctggtttac aggattttct gacctaaatg tctctactat
     6661 ctgattggtc tgtgagattc ttgatatata ttgcagtggt tttttcccga atgataatgt
     6721 aaatggattt tgcattttgt tttcccctta tataacttta tataactttt tgtttcgtat
     6781 ataagtttat ataaccttat ataactttgc atttattata taactttata taacttttgt
     6841 caccataaat tcttaatctt taaaagcatc tttataagat attttatcta ccagcgcttc
     6901 ataactgcta tcatcagctt gtactatctc tctgacattg tgctgctcta atgctgctgt
     6961 tgttttttca cctattgagt atgctgtttc cacgtatgca ttctccggca gcatatgaaa
     7021 aaatctttcc acatttgatg cgcttgtaaa aacagcttca cttaccatgt ttccaatgct
     7081 ttcaggaata ctctcatatg cctgctcatt tacatatgca tcaatttttt ctaattgaca
     7141 tatttcttta agccctgcct caaggtcagg tgaagcctcc tttgccgaga aaatgcagac
     7201 tttgatttca tcattatcgc ccatatatga actcaattct cctgcaagtt cttttccggt
     7261 ctgttctgcc ggaataatat ctgcatttat gtgatgcttt gcaagctctt tttctgtagc
     7321 ttttcccact actgcaaacc ttgcatttgc tataagcctg atgtctgcac ctgccagctc
     7381 cagattatag aaaaatgatc ttacaccgtt tttgcttgta aaaactatcc agtcagccga
     7441 tcttacctta ttggcaaaat caggaattat tatcgggttt attatgcctg tcttaaccgg
     7501 tgtgacatct gcnnnnnnnn nnnnnntctg ctccaagctg ctgtaatttt gctatcaatt
     7561 cgtttgtttt gatatacggt actgtgattt ttcttccaaa cagcggtctt ttttcaaaga
     7621 aatcaagcct gtcattcagt gaaaccacat cccccacaac gattatagcc ggtgatgtca
     7681 gctttgcttc ctcgattttt tctccgatat ccgcaagcgt tcctatacat ttgcgctgcg
     7741 cagcaagtgt gccgttcgat atcactgctg ccggagtatc cgatcttcgt cctgctttca
     7801 taagccctgc tgcaatgctt tttacatgcg caagtcccat caaaaataca caggtgattg
     7861 tctcatctgt aaggagggaa tagtctatat ctgcctcctc atcctttctg ctgtgcgcag
     7921 tgatgacctg aaagcctttt gctatgcctc tgtgtgtgat aggtattcct gcagccgcaa
     7981 gcgctgcaac cgatgaactg acacccggta ccacttctac ctccacattt ctatctacta
     8041 ggtagagagc ttcctctccg cctcttccaa agacatacgg atctccgccc tttaaacgga
     8101 ctacaagctc atgatatttt gatttctcgt acaaaagctc gtttatggca tcctgcttca
     8161 tgacatgctt atgattttcc ttgcccacat aaataagctc acagcctgcc tttgccattg
     8221 aaagaaactc tggtgatgat agcctgtcat acaccagaca atccgcctgt tttatatatt
     8281 tttctccttt taaagtgagc aggcccgtat ctcccggtcc tgctccaact aatactactt
     8341 ttcctgtgtt ttctctcatg attttcctaa ttatttttct actgaattat tatgattaaa
     8401 aattatgact tgatattttt ttagtcgatt tctaatttat accgaatatc gtgcaccgca
     8461 cgctccacga tattgtttct gtctgatgta atatcccata cctgctctgt ccttgcaagc
     8521 ttttttccat cctctgagcc aaacatcgca agaagcttta gcctgttatt tttgttgctg
     8581 tcattaccgc ggtctttttc acttacaata tcacagtatg cagccacagg cagatgacag
     8641 tttccaccga ttcgctttaa aaattctctc tcaatgcctg cgcacagatt ggttttctca
     8701 tctgacaaag catcaagctt tgccatcagc ttagcattgt cagcacgtag ctcaagcgcc
     8761 agtgtgccct gtgccggagc cggaatcata tcatcctccg aaaagtactg tgtgatctca
     8821 gaatctcttc cgatacgctt tattcccgcc gctgcaagta cgattccatc cagcctgtat
     8881 tcgttatact tattctgttt ttcgttagct atctcattgt tatttacagt attatcggca
     8941 atctcttcat atataatttc accatcatat agcttacgaa gcctcgtatc gatatttccg
     9001 cgtatgccaa ccaccttaag atcaggacgc agctttaaaa gctgatactt tctgcgcttg
     9061 cttccggttc ctatcacggc accatgagga agctcatcca gtgaagcggc attttttagc
     9121 accaaaacat cccttggatc ttcacgcttc catgcctttg caaatgtaag ccctgctgcc
     9181 ggttcatcag gcatatcctt catggaatgg actgccatat gaatctttcc cgataaaagc
     9241 tcgtcctcta tctcctttac gaagataccc tttgagccaa tcttatcgag tgcctttttc
     9301 tgctctaaat cgcctgttgt ctttatgata acagccttaa aatcatcatc cggatatgct
     9361 ctttttaact tatctatcac gtaatcagtc tgaacaagag caagctttga gcctctcgtt
     9421 cctattaaat acctcatttt attctcctac ggatcatgcc attttccatt tatcatttat
     9481 aaatgaacaa caacttaaaa tctgtaatac catactttaa actatatttc cggaatcctt
     9541 ccattatcaa tagctgcaca caatatttct ttaaacacac gctttctctc ggcttgagtg
     9601 tcatattgcg ctattacttt ttcgcggatc tgtcccatgt attcgttcag ctcaccaaga
     9661 aacggagtaa gaatctcctg ctctttgcct ttcagatact gagtcagcac agggctgttt
     9721 cctccgctcg aaaatgccgc aacaagattc ttttccttta tgtatgacgg aaaaataaag
     9781 ctgcaatcag ccttctgatc gaccgcattt accatgatgc cattagcttt gcaatactct
     9841 gcaatctcat gatttaaagc attgtcatca gtggcagcga taaccatttc catgccatca
     9901 cagtcttttc gttcaaacct tctctttatg aatgtaattc tatctgctgc atccgagtcc
     9961 gtctgattgt tttctttatt tgccgtatat gagcccgttt tatcctcgct tgctatatca
    10021 tcaattgtaa gctttctcag ctcatcacat atatcctctg ccacaaccgt gaccttcgcg
    10081 ccaaaatcca gcattacaat caccttgcgg tatgccacat ttccaccgcc gacgatcaga
    10141 cactctcttt ccgtcatatc cacaaacatc ggaaaatacg ccatttttat ctccctgtaa
    10201 taccatatcc atactttatt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattccttca
    10321 aaaacaggac gaagcttagc agaaaacact gctgccgata cgcataatat aaagtccggt
    10381 gcaacagtta tcagacagta atctactatt acgatgcccc agctgacagg tgttgccgca
    10441 tagaaattct tcatgcagta aaagtatatt gctccaactg tataatatgc caccagtccg
    10501 accgttgccg gaagaatgag tgtaaacgca ttggtctttt taagcttttc tgtaatcgct
    10561 ccgattaaaa atgcggcaag cacaaagcct aagaggaatc caaagcccgg tcttaaaatg
    10621 tatgtaggac caccgcctgc cgcaaataca ggcactccca ccagtccgat gcacaaatat
    10681 actatcacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttgcccc gagcagaaat
    10741 cccgccatca gcacaaagaa ccactgtaat gtaaaatgca tggtgtacat aggaagcgga
    10801 atatcaatct tgataaatgc tccaactgct ataagtgccg caaaaagcgc acatgttgta
    10861 agttccttcg tttttaattt cattttcgnn cgctctttcg ttttattaaa tatacatgct
    10921 ataataacat ctttctctaa aaaagaaaag ccatcttcga tgaagctgac ttttctttgc
    10981 agctttcgct gcgactcaaa acattaacat atgatttaga agtaatattc atcagaatat
    11041 attcgtaaat cctctttcgt gacctgtcca ccctcgatat gaaacgagtt gattacagga
    11101 ttttctgcca taagcgacat tatcatgtag ctcgcatttg gatcatatgc aagctttata
    11161 tcctcatccg atggccttga agggctagac ggatgtgaat gccagttgcc aagcggctta
    11221 aggccgtttg ctctcatgtc ctttatggct gccagctgct cctttggatc cattgtgaaa
    11281 tggtcctcgg catggtctgt attcgtcagg taatatacct ttgtgatgag acgaccgttt
    11341 tcgtcctcac tgcctgctat cagaccgcac gcctcctccg gcagatgctc tttagcatat
    11401 tttgctattt catccagaaa cggatatttc attcttatga tactcatatc gttcctttcc
    11461 ttactgtctt tttattcctg tgtacgaggt gcatcaaatc tgcccgctgt ctcctcgcag
    11521 acctcctgct cgtagtcgat tggctcgaga atggtcggat ggtcgccaca gactgcacag
    11581 ttgtgtgtat cctttggaag cttaatcttg tggaactcca tggtgagtgc atcatatgta
    11641 agcagatatc ctgtgagcag ctctccaaca ccgattaaat acttgatagc ctccatagcc
    11701 tgaagcgaac cgattactcc gcccatagct ccgataacgc ctgcctgctt acatgtaggc
    11761 actgcatcct ttggtggcgg attcttgaat acacatctgt aacaaggtcc ctctcccggc
    11821 acatatgtca tgagctgacc cttgaaacgg atgattcctg catgtgaaaa cggctttttc
    11881 tcaagcacac atgcatcgtt gattaagaac tttgccggga aattgtcagt tccatcgatg
    11941 atgaaatcat aatccctgat aagctctctg atgtttgacg catctacaaa catacggtat
    12001 gtattgacct gaacatccgg gttcatggcg ttcattgtct cttttgctga ctttaccttg
    12061 gctttgccca catcggctgt tccgtggata atctgtctct gcaggttgga taaatctacc
    12121 tcatccgcat ccacaatacc gattgtgcca actcctgctg ccgcaagata catggctgcc
    12181 ggagctccaa gtccgccggc accgataata agcaccttgc cctttaataa tttcttctga
    12241 cctttaacgc ccacctcatt aaggataatg tggcgtgaat agcgttcgat ctgctcatca
    12301 gtcattgcca ttagcatcca cctcccatga agtaaaggaa ctctacattg tcgccatcct
    12361 taagcactgt ctttggctta tcctctgtgg caacaaattc ctcatttatt gaaactgtta
    12421 catactcagg catatctaca ttctcaagct cgataagctc cggtaatgta agtccatcct
    12481 tatattcttt cttctctcct gctactgtga tagtcatttt atttcctcct gtgtcacaga
    12541 ttgtggtatc tgtatgtgtt attatcctgt atcagactgc tttatataat aatctaaaga
    12601 ctataataat tcttcaaccc acgccttcaa atcggcaggc ttttgtgcgc cgacctttct
    12661 atctaagacc tctgcgtctt ttacgaaaag cagtgtcgga ttcgcagtca cctcatactc
    12721 atcagcaagc tctccgtcaa agtttgtgtt
//
LOCUS       DPUF01000036            5301 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3227, whole
            genome shotgun sequence.
ACCESSION   DPUF01000036 DPUF01000000
VERSION     DPUF01000036.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3227"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="DHV81_01315"
     CDS             complement(<1..795)
                     /locus_tag="DHV81_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI93177.1"
                     /translation="MEKSIRKKFWIFALPTMAAFTIGFIVPFVYGIFLSFCKFSTVTD
                     WKWVGFKNYTRIFVVNGSVDTTFIHSIWYTALFTVVSVLIINIVSFAIAMALTKGIRG
                     TNLFRTVFFLPNLIGGIVLSYVWLMIFNSILQNYSKTIVSSEWSAFWGLIAVVCWQQI
                     GYMMIIYIAGIQNIPGELIEAAKIDGANRWQLLKSVILPMLMPTITICTFLTLTNGFK
                     LFDQNLALTGGNPGKMSELLALNIYNTMYGTTGWQGVGQAKAVIFFI"
     gene            complement(883..2271)
                     /locus_tag="DHV81_01320"
     CDS             complement(883..2271)
                     /locus_tag="DHV81_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI93178.1"
                     /translation="MKKKLVTVLLASSMVATMLAGCGSGSGDGTEKGSSAKGGDGSVY
                     MLNFKPETDQAWQDLAEKYTDETGVQVTVVTAADGQYKTTLKSELAKKEAPTIFNIGS
                     TSDCAEYDKYIYDLKDSEIYKHLTDKSLALEYNGKIASVANCYECYGIIYNKAILEKY
                     CSNYSGAVIKSVDDIKDLDTLEKVATDINEHVDDINKACDLHLTEAFASAGLDSGSNW
                     RFTGHLAGLALYYEFKDAGCDLTAGQKEVTGKYMDNFKRVWDMYTNTSAADKATLDSG
                     SLNAESELGMEEAVFYQNGDWEYANFADDNENGYTVKQSDLSMMPIYFGVDDANEGLA
                     VGTENHWTVNAKADQKDIDATLEFLNWVITSDDGRDAIVNKMGLSAPFDTFTGDYESK
                     NAFANVASELAKEGKTSVAWSFNATPSVDDWRADFLAPLTEYTERNGSWDDVAKAFSE
                     KWAYYWDLQNEQ"
     gene            complement(2520..2804)
                     /locus_tag="DHV81_01325"
     CDS             complement(2520..2804)
                     /locus_tag="DHV81_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93179.1"
                     /translation="MDENYTYIVRCADGTLYTGWTNDLKKRIKAHNSGKGAKYTKTRR
                     PVELVYFEHFATKEEAMSREYHIKQLKHAQKQALIDEMNKNNKSIFTQNG"
     gene            complement(2797..3072)
                     /locus_tag="DHV81_01330"
     CDS             complement(2797..3072)
                     /locus_tag="DHV81_01330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93180.1"
                     /translation="MEFNEYNESVKNWTNGILDNYRKDAELTIRYCHELIDYGEKTAD
                     SKLLGFGYYHLAMTLYCLNDYDNIFDIVVRAIDHLEKAQSGRQSRYG"
     gene            complement(3082..3759)
                     /locus_tag="DHV81_01335"
     CDS             complement(3082..3759)
                     /locus_tag="DHV81_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HCI93181.1"
                     /translation="MKKQQIAAAACLVAGAIGFAAVYSTQKPGKDEIPTEISQTSSIV
                     EPTEKKTPQKTTEKVDSETKMGQEESAAGQQENQQSTEKQLHFVPENVTNWPVKGDVL
                     LPFSMDKTVYFPTLDQYQYNRGMVIRANEGDAVCSVTEGRIIDIYDSAETGCTVVQDL
                     GDGYTATYGQLANLTCSEGDVLEAGETLGTVGKVTRYYTVEGTNVYFAMEQDGKPVNP
                     MDYFGDI"
     gene            complement(3834..4700)
                     /locus_tag="DHV81_01340"
     CDS             complement(3834..4700)
                     /locus_tag="DHV81_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCI93182.1"
                     /translation="MAYLGNPKNTIEVLQKYNFNFQKKFGQNFLIDTGILEEIIDSAG
                     ITKDDMILEIGPGIGTMTQYLCESARQVIAVEIDTNLIPILKDTLSEYDNVKVINDDI
                     LKVDINALAEEYNNGKPIKVVANLPYYITTPIIMGLFESHVPIDSITIMVQKEVADRM
                     QAGPGKKDYGALSLAVQYYSHPEIVVNVPPSCFMPQPKVGSSVISLKRYEKPVVDVDD
                     EKLMFKIIRASFNQRRKTLANGLNNFGGMGLTKEQIQQSIEALGVPVNVRGEALSLAQ
                     FAQLSNIIGAAK"
     gene            complement(5128..>5301)
                     /locus_tag="DHV81_01345"
     CDS             complement(5128..>5301)
                     /locus_tag="DHV81_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase TatD"
                     /protein_id="HCI93183.1"
                     /translation="ETDCPYMAPEPHRGTRNDSSNIPYVISKIAEIKGTTPDEVERKT
                     RANAFELFTRVNN"
BASE COUNT         1282 a         1256 c         1015 g         1748 t
ORIGIN      
        1 tataaagaat ataacagcct ttgcctgacc tacgccctgc catcctgttg ttccatacat
       61 ggtgttgtag atatttaagg caagcagctc tgacatcttt cccggattac cgcctgtaag
      121 agctaagttc tggtcgaaca gcttgaagcc gtttgtcaat gtcaggaatg tacagattgt
      181 gattgtcggc ataagcattg gaagaataac gctctttaag agctgccatc tgttggctcc
      241 atcaatcttt gctgcctcaa taagctcacc cggtatattc tgaatacctg cgatgtagat
      301 aatcatcata tatccaatct gctgccagca tactactgcg ataagtcccc agaatgctga
      361 ccactctgag cttacgattg tctttgaata gttctgcagg atagagttaa aaatcatcag
      421 ccatacatat gaaagtacga ttccaccgat caggtttgga aggaagaata ctgtacggaa
      481 aagatttgtt cctctgattc cttttgtaag cgccattgct attgcaaatg atacgatatt
      541 gataatcagt accgaaacta ctgtaaatag tgctgtgtac cagattgaat gaataaaggt
      601 tgtatccaca ctgccgttta caacaaaaat ccttgtgtaa tttttaaatc ccacccattt
      661 ccagtctgta acagtggaga atttacaaaa tgaaaggaag ataccatata cgaacggtac
      721 aataaatcct attgtaaatg ctgccattgt cggtaaagcg aagatccaaa atttttttct
      781 aatcgacttt tccataacaa atataccttt cttaaaaaaa tatggggtga gtgactgcca
      841 ctcaccccac ttttactact ttttactaac ttttagagaa ttttactgct cgttctgaag
      901 atcccagtaa taagcccatt tctctgagaa tgcctttgca acgtcatccc atgaaccatt
      961 acgctctgtg tactctgtga gtggagcaag gaaatcagct ctccagtcat ctactgaagg
     1021 tgttgcgttg aatgaccatg ctactgatgt cttgccttcc tttgcaagtt ctgatgctac
     1081 attagcaaaa gcgttctttg actcatagtc gcctgtgaat gtatcgaatg gagctgaaag
     1141 acccatttta ttaacgattg catcacgtcc gtcatcactt gtgattaccc agttgaggaa
     1201 ttcaagtgtt gcatcaatat ccttctgatc tgccttagcg tttactgtcc agtggttctc
     1261 tgtaccaact gcaagtccct cgttcgcgtc atctacaccg aagtagatag gcatcattga
     1321 aaggtcagac tgcttaactg tgtatccgtt ctcgttgtca tctgcgaagt tagcatactc
     1381 ccagtcacca ttctggtaga atacagcctc ttccatacca agctctgact cagcatttaa
     1441 gcttcctgaa tcaagtgtag ccttgtctgc tgctgatgta tttgtgtaca tatcccaaac
     1501 tctcttgaag ttgtccatgt acttacctgt aacttccttc tgtcctgctg tgagatcaca
     1561 tcctgcatcc ttgaactcat agtagagtgc gagacctgcg agatgtcctg tgaatctcca
     1621 gttagatccg ctgtcaagtc ctgctgaagc aaatgcctct gtaagatgta agtcacaagc
     1681 tttgttgatg tcatctacat gctcgttgat atcagttgca accttctcta atgtatcaag
     1741 atccttgata tcatctactg acttgataac tgctccagag tagttgctgc agtatttctc
     1801 aagaattgcc ttgttgtaga tgattccgta gcactcatag cagttagcta ctgatgcaat
     1861 cttgccattg tactcaagtg caagtgactt atctgtaagg tgcttgtaaa tctcgctgtc
     1921 ctttaagtca tatatgtatt tgtcatactc tgcgcaatct gatgtgcttc cgatattgaa
     1981 gattgtagga gcctccttct tagcaagctc agacttaagt gttgtcttgt actgtccatc
     2041 agctgctgtt actactgtaa cctgtacgcc tgtctcatct gtgtacttct ctgcaagatc
     2101 ctgccatgcc tgatctgtct ccggcttgaa gtttaacatg taaactgagc catcgccgcc
     2161 ctttgcagat gagcctttct ctgttccgtc tccgcttcct gatccacatc cggcaagcat
     2221 tgttgctacc attgaagaag cgagtaatac tgttactaat ttctttttca ttttaaaccc
     2281 tcctaaaaga tttaatatta aaattgtctt tgcaaacatt attggttgtt ttcggtaatg
     2341 tttgcggtaa cgttttcaca agaattatta tagacagttt catgtagttt cgcaagagga
     2401 ttttgtcttt tctgtatata ttaataaaaa caccaggtat tttttggcaa tttttcacaa
     2461 agaattgcaa aaaaataagt attctcacac tggcgtttgg tcattttagt tattttcgac
     2521 tatccgtttt gtgtaaaaat acttttgtta tttttattca tttcatctat cagagcctgc
     2581 ttttgtgcgt gcttgagctg ttttatatga tactccctgc tcattgcctc ctctttcgtg
     2641 gcaaagtgct cgaaatagac cagctccaca ggtctgcgtg tctttgtata tttggcacct
     2701 ttcccggagt tatgcgcttt tatgcgcttt ttcaggtcat ttgtccaacc ggtgtaaagt
     2761 gtgccgtcag cacaccttac aatatatgta taattttcat ccatatctgc tctgtcgacc
     2821 actctgcgcc ttctccagat gatctatcgc cctgaccacg atatcaaaaa tgttatcgta
     2881 gtcattcagg caataaagtg tcatggcaag atgatagtat ccgaagccca ggagctttga
     2941 atctgctgtc ttttctccgt aatctatcaa ctcatggcaa tatctgattg tgagctcggc
     3001 atcctttctg taattgtcaa gaatgccatt tgtccagttt ttcacactct cattgtattc
     3061 gttaaactcc acttttcacc tctatatatc tccgaaatag tccataggat tgaccggttt
     3121 tccatcctgt tccatagcaa aatacacatt ggttccctca acagtatagt atctggtcac
     3181 ctttccaact gttccaagtg tttctcctgc ctcaagtaca tcgccctcac tgcaggttag
     3241 atttgcaagc tgtccgtaag tagccgtata gccgtctcct aaatcctgca ctactgtgca
     3301 gcctgtctct gcgctgtcat atatgtctat aatcctgccc tcggtaactg aacataccgc
     3361 atctccttcg tttgcacgta ttaccatgcc acgattatac tgatactggt cgagtgtcgg
     3421 gaaataaacc gtcttgtcca tggaaaacgg caaaagcaca tctcccttta caggccagtt
     3481 tgtcacattt tccgggacaa aatgcagttg tttttccgtg gactgctgat tttcctgctg
     3541 ccctgcagct gattcttcct gtcccatttt tgtctctgag tctacttttt ctgtagtttt
     3601 ctgtggtgtc tttttctctg taggctcaac tatagacgaa gtctgtgaaa tttctgttgg
     3661 tatctcgtct ttcccgggtt tctgtgttga atataccgct gcaaatccta tggctccagc
     3721 cacgagacat gccgctgctg caatttgttg ttttttcata ggacattacc tccatcatct
     3781 gaattgttct gatgatagta tgtcctgtct gcatggattt attcatgctt tttctatttt
     3841 gcggcaccaa ttatatttga taattgcgca aactgtgcaa ggcttagagc ctcaccacgc
     3901 acatttacag gaactccgag agcctctata ctctgctgta tctgctcctt ggtcagtccc
     3961 ataccaccga aattgttgag cccgttggca agagttttcc tgcgctggtt gaaagatgcc
     4021 ctgatgattt tgaacatcag cttttcatca tctacgtcaa ccaccggctt ttcgtatcgc
     4081 ttgagcgata taacagagct tcctaccttt ggctgtggca tgaaacagct tggcggcaca
     4141 tttaccacta tctcagggtg cgaataatac tgcactgcaa gtgacagagc tccgtaatcc
     4201 ttcttgccgg gacctgcctg catacggtct gcgacctcct tctgcaccat aattgtaatg
     4261 ctgtctatcg gcacatgact ctcgaacagc cccataataa taggtgtcgt tatgtaatat
     4321 ggcaggtttg caacaacctt tatcggctta ccattattat attcttctgc cagagcattt
     4381 atgtctacct ttaatatatc atcatttatg acctttacat tatcatattc actcagagta
     4441 tccttaagta taggtatcag atttgtatct atctcgactg ctattacctg gcgcgccgac
     4501 tcacacagat actgtgtcat cgtacctatt cccgggccta tctcaagtat catatcgtcc
     4561 tttgtgatgc cggccgagtc tatgatttcc tccaaaatgc ctgtatcaat aaggaagttc
     4621 tgtccgaatt tcttttgaaa attgaaattg tatttctgca gaacctcgat tgtgttctta
     4681 ggatttccca aatatgccat ggtatgcgct ccttgtgtcg tttcatctga tgcgacagta
     4741 caactgctgt cattatcttt tatattatca catgaaatgc ctttatttca attatatttg
     4801 taaattttcg cggttttgcc aaaacccggg atgaaagccg ggaaccgccg ccctgccatg
     4861 gggcacggta cactctcgcg tgagctacgc ttcgcagtta agattctgta cggattatgt
     4921 ctaaggattt tctatgcttg acgaaaccgg cttttatgcg ccgtccgtgg cgcataacgc
     4981 cttgcccagc cgtccatggc tgggcatttc tgcgtattta aacataatcc tacagtatct
     5041 aaacagcttc gctattcgct cacgcgggag tgtaccgtgc cccatggcag ggcggcggtt
     5101 cccgactttc ttccctcacg ttgtggctca attatttaca cgtgtgaaca gttcaaatgc
     5161 attggcacgg gttttgcgct ctacctcatc aggcgtagtg cctttgattt ccgctatctt
     5221 tgatatgaca tatggaatgt ttgacgaatc attcctcgtg cctcgatgtg gctccggggc
     5281 catgtatgga cagtccgtct c
//
LOCUS       DPUF01000037            3576 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23465, whole
            genome shotgun sequence.
ACCESSION   DPUF01000037 DPUF01000000
VERSION     DPUF01000037.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23465"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="DHV81_01350"
     CDS             <1..444
                     /locus_tag="DHV81_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93184.1"
                     /translation="QLLAMYIGYFLSSFLNGKYSVKDEALLGEIISIGILIGLGIRLI
                     GKAIRNERIEEHCQAQLDMKRVLRIAGITAFYTVLAGIAFGFLGTGLFTIMIMVALLT
                     VVCIVAGIYTGYHFGFEQKTKVYIVGAILFWAAGIDVIVRLVSAK"
     gene            741..1577
                     /locus_tag="DHV81_01355"
     CDS             741..1577
                     /locus_tag="DHV81_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HCI93185.1"
                     /translation="MGREENVLIFQDTERLSKTNGKLNADLKASASGQKLILETDALE
                     KPNLNIYDEPAKVIVSKKRTFEAAKAYRDNKVAVHNFASASNPGGGVVRGSTAQEECL
                     CRCSYLYFCLNSPDMWDGFYTPHKQARNPLHNDDIIYTPEVTVFKSDTNNPALLPENE
                     WYKVNVITCAAPNLRAMPSNKHNTGDGKKAAKISDKEMLEIHEKRLRRILDVAVSEGN
                     DTVILGAFGCGAFMNKANIVAYAAKNVIKDYMHAFKNIEFAIYCSPQDDTNYKTFRLI
                     EH"
     gene            1709..1921
                     /locus_tag="DHV81_01360"
     CDS             1709..1921
                     /locus_tag="DHV81_01360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93186.1"
                     /translation="MDEDLQAEYKGALDTGLFDIIIFVYESWFVNHRLILTSEPVDLR
                     RAIYRGWMMKPDEYENFYNMLLNHIR"
     gene            1937..2929
                     /locus_tag="DHV81_01365"
     CDS             1937..2929
                     /locus_tag="DHV81_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93187.1"
                     /translation="MVYLTGDTHNEFTRLSNKYFKKYDWEIGENDYIIVCGDLGLCWS
                     KDKTFEWNCKWFAEKPYTLLWVQGNHENYDMIDEYPIEKWHGGNVRHIVRDKVILLER
                     GQIFNIEGKTFFTFGGASSHDTHGGILDRTSCEFEFMVQRARSLYLPYRIIGESWWSQ
                     ELPSEEEMQEGLLNLQKTDYKVDYVITHCCATELQNKIMSYVDGNSKPDILTDYLQEL
                     ESKLEYKHWYFGHYHHDFNVDENHTLLYKKIINLDEQLPEYGRVPIIGMPKFKRNDMV
                     VFKFRDDEKCGMIQIVDAYGTFEQDDEPSYDICVEEENCLYKHIRETDIVRKAC"
     gene            3191..3460
                     /locus_tag="DHV81_01370"
     CDS             3191..3460
                     /locus_tag="DHV81_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI93188.1"
                     /translation="MNYPVLDLKATGERINQLRKDNNLRVIDVAEYMGFESTQAVYKW
                     QRGESLPTVDNLYALSRLLHTSVDDILIGEKERDDEPSLSFCLCA"
BASE COUNT         1221 a          507 c          858 g          990 t
ORIGIN      
        1 cagttgttag caatgtacat cgggtatttc ctgtcaagct tccttaatgg gaaatactcg
       61 gtgaaggatg aagcactttt aggagagata atttctatcg gaatactgat aggactgggg
      121 atcaggctta taggaaaggc tataagaaat gaaaggattg aggagcattg tcaggcacag
      181 cttgacatga aaagagtgtt aaggatagca ggaatcaccg cattttatac ggttcttgca
      241 ggaatagcat tcggctttct tggtaccgga cttttcacca ttatgataat ggtggcgctg
      301 ctcactgttg tctgtatagt ggcaggcatt tacactggat atcacttcgg ttttgagcag
      361 aagacaaagg tatatatcgt gggggcgata ctcttttggg cggctggaat tgatgtgata
      421 gtaaggcttg taagtgcaaa ataatgcata gggctctgtt cgtaaggaca gggctttttt
      481 gctgtccatt gaagtatgaa atactttgct tgaaataagg tgtatacttc tcatgaaaat
      541 agtttctcga agttggtgta ttatcattat gtcagcaaga actgacatga aaaaagtaaa
      601 ccacaggttt attgaaaaaa caggatgaag aaaataaaat taacagtaga cggaattgta
      661 aggtataatg gaaggagaga cagctatggc atagaaaatg ctggtaacac aggcattttt
      721 taaagaaatg gagaatcatt atgggaagag aagaaaatgt attaatattt caggatacag
      781 agagattaag caaaacaaat ggaaagctta atgctgactt aaaggcatct gcaagtggac
      841 agaaactaat attggaaaca gatgctttgg agaaaccgaa tctaaatata tatgatgagc
      901 ctgcaaaagt gattgtttca aagaagagaa cgtttgaggc tgcaaaggca tatagagata
      961 ataaggtagc tgtacacaat tttgcatctg cttcaaatcc gggcggaggt gttgtaagag
     1021 gttcaactgc ccaggaggag tgcctgtgca ggtgttcata tctttatttc tgtctgaatt
     1081 cacctgatat gtgggatgga ttttatacac ctcataagca ggcaagaaat ccattacata
     1141 acgacgatat catttataca ccagaggtaa ctgtatttaa aagcgataca aataacccgg
     1201 ctttattgcc agagaatgag tggtataagg taaatgtaat cacttgcgca gcaccgaatt
     1261 taagagccat gccaagcaat aaacataata caggcgatgg caaaaaggca gcaaaaatca
     1321 gcgataagga aatgcttgaa atacatgaga agagattaag acgcatactg gatgttgccg
     1381 tttctgaagg aaacgataca gtaatacttg gagcatttgg atgtggagct tttatgaata
     1441 aagcaaatat cgttgcatat gctgcaaaaa atgtgattaa agattatatg catgcattta
     1501 aaaatatcga gtttgcgata tactgcagcc cacaggacga tacaaactat aagacattca
     1561 gattgataga gcactaggca aaaatgaaat atgtttgaat gtcaatacat atgaacttat
     1621 aagaaataat gtagcatttc aggcagagga tatttaaatg aaggtagatt tgataatatt
     1681 tccgtccagt tatttttcaa agaattaggt ggacgaggat ttacaagctg aatataaagg
     1741 ggcactggat acagggctat ttgatataat catatttgta tatgagagct ggtttgttaa
     1801 tcataggctg atacttacaa gtgagccggt ggatttgaga agggcgatat acagagggtg
     1861 gatgatgaaa cctgatgagt atgaaaattt ttataatatg cttttaaatc atataagatg
     1921 aaagatagag gaaaatatgg tctatttaac aggtgataca cataatgagt ttacaagatt
     1981 atcaaataaa tattttaaaa agtatgattg ggaaatagga gagaacgatt atatcatagt
     2041 atgtggtgat ctgggtctgt gctggtcaaa ggataagaca tttgaatgga actgtaagtg
     2101 gtttgctgaa aaaccatata ctttactatg ggtacaggga aatcatgaga attatgatat
     2161 gatagatgaa tatccaattg aaaaatggca tggtggcaat gttcgtcata tcgtgagaga
     2221 caaggtgata ttacttgaga gaggccagat attcaatatt gagggaaaga catttttcac
     2281 attcggtgga gcttcatctc acgacacaca tggcggaata cttgatagaa catcttgtga
     2341 atttgaattt atggttcaaa gggcaagatc attgtattta ccgtatagga taattggaga
     2401 atcttggtgg agtcaggaat tgccttcaga agaggagatg caggaagggc ttttgaatct
     2461 gcaaaagact gattataaag tagattatgt gataactcat tgttgtgcaa ctgagcttca
     2521 aaataaaatt atgagctatg ttgatggaaa tagtaagccg gacatattga ctgattattt
     2581 gcaggagcta gaatctaagc ttgagtataa gcattggtac tttgggcatt atcaccatga
     2641 tttcaatgta gatgagaacc atacgttatt gtataaaaag attatcaatt tggatgagca
     2701 actgccggaa tatggcaggg ttccaattat cggaatgcca aaattcaaac gcaacgatat
     2761 ggtagttttt aaattcaggg acgatgaaaa gtgtggcatg attcaaatag tggacgcata
     2821 tggaaccttc gagcaggatg acgagccatc ttatgatata tgtgtcgagg aagaaaactg
     2881 tctatataag catataagag agacggatat tgtaaggaaa gcatgttaaa cagatactca
     2941 acagatattt atttggaata tgaagttcag ttaattcttt ctgaaatata ggcggttgaa
     3001 gatattctta aagaaaataa aaaatcttta caaataaaat gaattgatgt attatagcac
     3061 cacacaaaac gtagcaatcg ataataatag ccttacatcc attgaaaaac agcggtgtct
     3121 gatgcgtatg cagaggatgt tgtaattagt aagtgacgta ccgaacgaat ttacacaagg
     3181 aggcattcat atgaattatc cagtattaga tcttaaagct accggagaac gaatcaatca
     3241 gctccgcaag gacaataacc tcagggtgat tgatgttgcg gagtatatgg gcttcgaatc
     3301 aacgcaggcc gtttacaagt ggcagcgtgg tgagagcctt ccgacggtcg acaacctgta
     3361 tgccttgagc aggctgttgc acacttcggt ggatgacata ctgataggcg agaaagagag
     3421 ggacgatgag ccctctcttt ctttttgctt gtgcgcctag cggcgcacgt ttctaacggg
     3481 ttaaagtccc gaatccgccc ggtagtggga aggatatagc cgaaagcaag ggtgtccatc
     3541 gtgagatgga atctgaagga agctggatgt gaggaa
//
LOCUS       DPUF01000038            5313 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_9549, whole
            genome shotgun sequence.
ACCESSION   DPUF01000038 DPUF01000000
VERSION     DPUF01000038.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_9549"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(74..547)
                     /locus_tag="DHV81_01375"
     CDS             complement(74..547)
                     /locus_tag="DHV81_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCI93189.1"
                     /translation="MTQDEKYMKQAIKQAKKAYALDEVPIGCVIVAGDKIIARGYNRR
                     NTDKNPLAHAELSAIRKASKKTGDWRLEECTMYVTLEPCQMCAGAIVQARIPRVVIGA
                     MNPKAGCAGSIINLLQMQQFNHQVQTDIGICGDECSTMLSTFFKELRKKRKLQGE"
     gene            612..1157
                     /locus_tag="DHV81_01380"
     CDS             612..1157
                     /locus_tag="DHV81_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCI93190.1"
                     /translation="MFFEYETPRLLLKVLRADCAPQVLDFYNRDKELFEMYEIDRVPD
                     FYTVKHLQKVLRYEYNMAVKSQLFRFYVFQKENPCRIIGTVCIHNIAPAFYGSCEIGY
                     KFSSEFHHMGYATEAVGFCTKLAFNELRMHKVTAVVHPGNVPSMKLLNRLGFVQEGLL
                     HDYLILHGKWCNHYIYGLVNK"
     gene            complement(1225..2193)
                     /locus_tag="DHV81_01385"
     CDS             complement(1225..2193)
                     /locus_tag="DHV81_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HCI93191.1"
                     /translation="MKGKHKIVVKNNRLHYEFEIKRNITIIKGDSATGKTTLINMIRQ
                     FANLGNASGIEIECDAPCTVLEGNLWQMLLKNLSGNIIFIDEENQFIRQQEFAELVKA
                     SDNYFVIITRENLYNLPYSVEEIYGLYSSGKYQNTKQIYQEMYHIYPLNQDLSCKPEK
                     IIVEDTNSGYEYFKAISKEKNIVCESAGGKTKIFAMLEQLKTETESICVIADGAAIGP
                     EMDALYKMSVEKGNIKLYLPESFEWIILSSELLEDKEIKDIMDKPENYIESQEYFSWE
                     RFFTKLLVDKTAGTYLKYQKGKLNPTYLHEKNKNIILKNIKNSIDF"
     gene            complement(2202..2639)
                     /locus_tag="DHV81_01390"
     CDS             complement(2202..2639)
                     /locus_tag="DHV81_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4869 domain-containing protein"
                     /protein_id="HCI93192.1"
                     /translation="MLNIYLGDMENAIYHPPVYFDNTYEDEWITDELSVKMIKDIDKS
                     DVIGPHLVQSPILGPISTKELSGGVKTLMLMAFDDSGKIFNASACGDNCAKWILRIAG
                     KKNLTINLHHIMDFGKDFEAKILNTGEIVHSMAEFISVAGKYV"
     gene            complement(2715..3548)
                     /locus_tag="DHV81_01395"
     CDS             complement(2715..3548)
                     /locus_tag="DHV81_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCI93193.1"
                     /translation="MEKKMNSIYEKLTTCLASVREKTDFVPETAIVLGSGLGDYAEQI
                     KIETTIDYKDIKGFPTSTVPGHKGRFVFGYVDSVPVVIMQGRVHYYEGYPITDVVLPT
                     RLMGMMGAKKLILTNAAGGLNTDFNPGDFMLISDHIATAIPSPLIGANIDELGERFPD
                     MSEVYSRRMRKVIKEKADKLGIKLREGVYVQLTGPQYETPAEVRMCKILGGDAVGMST
                     ACEALAARHMGLEVCGISCITNLAAGLSDKKLNHKEVQETADRVAKQFTELITAVVRA
                     V"
     gene            complement(3592..4779)
                     /locus_tag="DHV81_01400"
     CDS             complement(3592..4779)
                     /locus_tag="DHV81_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCI93194.1"
                     /translation="MKKYNRIFVIVLDSLGIGAMPDSERFGDTDVDTFGHILERMQTL
                     DIPNLTRLGMLNLHCGGQMQPAAEPIGRFTRLAEASNGKDTMTGHWEMMGIKTEKPFK
                     TFTEHGFPPELIAELEKQCGKKVIGNKSASGTEIIEELGEEEIKNGSMIVYTSADSVL
                     QICGNEETFDLQNLYRCCEIARKITLKDEWRVGRVIARPYVGKKKGEFVRTSNRHDYA
                     LKPTGLTALNALKDSGLDVISVGKINDIFCGEGITEAFRSKSSVHGMEQTIDICEKDF
                     KGLCFVNLVDFDALWGHRRNVTGYGEEIEKFDKNLGILMEKLRDDDLLILTADHGNDP
                     TYKGTDHTREYVPFIAYSKSMKGGGSIDEEATFAVIGATITDNFGVKMPEGTIGHSIL
                     EEL"
     gene            complement(4855..>5313)
                     /locus_tag="DHV81_01405"
     CDS             complement(4855..>5313)
                     /locus_tag="DHV81_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCI93195.1"
                     /translation="GFMELFKYAKQLGLPFTIHAGECGNAQNIIDAVEAGAVRIGHGI
                     AMRGHGELERQLSAKGIGIELCPISNLQTKAVASADEYPIREFLDAGLKVTINTDNRT
                     VSNTTLSKELEFIEKTYGIREEELPLMMKNALDVAFADDAVKERIFRQLM"
BASE COUNT         1276 a         1295 c          992 g         1750 t
ORIGIN      
        1 cttcgggaga gtacgtgtga gtccggtacc cggggcttac gtgccttacg ttgtggtttt
       61 aaccacaatt tatttactcc ccttgaagct tgcgcttctt tcgcagctcc ttgaagaaag
      121 tagaaagcat agtgctgcat tcgtcgccgc agatgcctat atctgtctgc acctgatgat
      181 tgaactgctg catctgcagt agattgatga ttgagcctgc acagcctgct ttcggattca
      241 tggcacctat taccaccctg ggaatgcgtg cctgtactat tgctccggca cacatctgac
      301 agggctcaag ggtgacatac atggtgcatt cctcaagacg ccaatctccg gtctttttgc
      361 tggctttacg gatggcagaa agctcagcgt gagcgagtgg atttttgtcg gtatttctgc
      421 ggttgtaacc gcgtgcgatg attttgtcac cggctacaat tacgcagcct atcggcacct
      481 catcgagtgc gtatgctttt tttgcctgtt ttattgcctg tttcatatat ttttcatctt
      541 gtgtcatatt tgtctgctcc atgtgttata atctatttat catgatacac caaatgtgaa
      601 agaaggaaaa tatgtttttt gaatatgaaa ccccacgcct gcttcttaag gtattgcgcg
      661 ctgattgtgc cccacaggtt ttggattttt acaataggga taaagagctt ttcgaaatgt
      721 atgaaataga ccgtgtgccg gacttttata ctgtgaagca cctgcagaag gtgctcagat
      781 atgaatacaa tatggctgtc aaatcacagc ttttcagatt ttatgttttt cagaaagaaa
      841 atccgtgccg gattattggc actgtgtgta tacataatat cgctccggct ttttacggaa
      901 gctgtgaaat tggctataaa ttttccagtg agtttcacca catggggtat gccaccgagg
      961 cagtcggctt ctgcaccaag cttgccttta atgagcttcg catgcacaag gtgacagcgg
     1021 ttgtccaccc tgggaatgtg ccttcgatga agcttcttaa taggcttggc ttcgtgcagg
     1081 aggggctatt gcatgattat cttatactac atgggaagtg gtgtaatcac tatatttacg
     1141 ggcttgttaa taaatgagct catttataat ttaacataac aattggcgca ggaaacaatg
     1201 tctgcgccaa tttatcatac ttttttaaaa atctatacta ttttttatat ttttcaaaat
     1261 tatattttta ttcttttcat gcaaatatgt aggattaagc tttccttttt gatatttcag
     1321 gtatgttcct gctgttttat ccacaagcag ctttgtaaaa aatcgctccc aactgaaata
     1381 ttcctgactt tctatataat tctcaggctt gtccataata tccttaatct ctttgtcttc
     1441 aagcaactca gaactgagta taatccactc aaatgattcc ggcagatata actttatatt
     1501 gcctttttcc acagacattt tatataatgc atccatttcc gggccaattg ctgcaccgtc
     1561 agctattaca caaatactct ctgtttcagt ttttagttgt tctagcatag caaatatttt
     1621 tgtttttcca ccagcgcttt cacacactat atttttttct ttcgatattg ccttgaaata
     1681 ctcatatcca gaatttgtat cttcaacaat tattttttca ggtttacacg aaagatcctg
     1741 atttaaagga taaatatgat acatctcctg atatatttgc tttgtgttct gatactttcc
     1801 tgaagaatac agtccatata tttcttcaac actatatggc agattgtaca ggttttctct
     1861 tgttattatc acaaaataat tatctgacgc ctttacaagt tcagcaaact cttgctgtct
     1921 tataaactga ttttcttcat caataaaaat gatatttcct gataaatttt tgagcagcat
     1981 ctgccacaga tttccctcaa gaactgtgca cggggcatca cattcgattt caattccgct
     2041 tgcattaccc agatttgcaa attgtctgat catatttatg agtgttgttt tcccagtagc
     2101 actgtcccct tttattatcg tgatatttct ttttatttca aattcataat gaagtctatt
     2161 gtttttcaca acaattttat gcttaccctt cataccttct cctatacata tttacctgca
     2221 acactgatga attcagccat actgtgaact atttctcccg tattcagtat ctttgcctca
     2281 aaatctttac caaaatccat tatatgatgc agattaattg tcagattctt tttgcccgca
     2341 attctcaata tccactttgc gcagttatca ccacacgctg aagcgttaaa aatctttccg
     2401 ctatcatcaa aagccatcag cataagggtt ttcactccac cggacaattc ctttgtggat
     2461 atcggtccca gtatcgggct ttgtaccaga tgtggtccaa tgacgtctga tttatctata
     2521 tcttttatca ttttgacaga cagctcatcc gttatccatt catcttcata tgtgttatca
     2581 aaatacaccg gaggatggta aattgcattt tccatatccc ctaaatatat atttaacatt
     2641 gaaatcctcc atcccatctg ttaggattct aaagtatacg ctatgatacg atttattgtc
     2701 aagtttctaa gcatttacac tgctctcacc acagcagtga tcagttctgt aaactgcttt
     2761 gccacgcggt ctgcggtttc ctgcacttcc ttgtggttga gctttttgtc ggatagtcct
     2821 gctgcaaggt tggtgataca tgagattccg caaacctcaa gccccatatg acgcgctgca
     2881 agtgcctcac aggctgtgct cattccgacg gcatctcctc cgaggatttt acacatcctg
     2941 acctcggccg gggtctcata ctgcggacct gtgagctgta cgtagactcc ttctctcagc
     3001 tttatgccaa gcttatctgc cttttctttt atgactttcc tcattctgcg gctgtatacc
     3061 tcgctcatat ctggaaaacg ctcgcccagc tcatcaatat tggctccgat cagtggactt
     3121 ggtatggctg tcgcgatatg gtccgaaatc agcataaagt ctcccggatt aaaatcagta
     3181 ttgagaccac ctgccgcatt tgtgagtatc agcttttttg cgcccatcat tcccatgagg
     3241 cgtgtcggta gcacaacgtc tgttatcgga tacccctcat aataatggac tctgccctgc
     3301 atgatgacaa caggcacact atccacatat ccaaatacaa acctgccttt gtgtccggga
     3361 actgtggatg ttggaaagcc cttgatatcc ttgtaatcta tggttgtctc tatcttaatc
     3421 tgctctgcat agtctccaag tcccgagccc aaaactatgg ctgtctccgg tacaaaatct
     3481 gttttttcac gcacacttgc aaggcatgtg gtaagttttt catatatact gttcattttt
     3541 ttctccatat attttctgtt aaagtttaaa aaattcctga gatattaccc cttaaagctc
     3601 ttcaagaata gaatgtccga tggtaccctc cggcattttt acgccgaagt tgtcagtaat
     3661 tgtggctcca atcaccgcaa aggtggcttc ctcatcaatt gagccgccgc ccttcatgct
     3721 ctttgaatac gcgataaaag gcacatattc gcgtgtgtgg tccgttccct tgtaggtagg
     3781 atcgtttccg tggtctgcgg tcagtatgag cagatcatca tcccttagct tttccattaa
     3841 aatgcccaga tttttgtcga atttctcaat ttcctctccg tagcctgtca catttctgcg
     3901 atgtccccac agtgcatcga aatcgaccag attcacaaag cacagccctt taaaatcctt
     3961 ctcacaaata tcaatggtct gctccattcc atgaaccgag ctctttgagc ggaatgcctc
     4021 tgtaatgccc tctccgcaga aaatatcatt gattttgccg actgaaatga catccagtcc
     4081 actgtccttt aatgcattta atgcagtaag acctgtaggc ttaagcgcat agtcgtgcct
     4141 gtttgaggtt cgcacgaact cacccttttt ctttcccaca tatggccttg caatgacgcg
     4201 ccctactctc cactcgtcct tgagtgttat ttttctggct atctcacagc agcggtagag
     4261 attctgtaaa tcaaaggtct cctcattgcc gcaaatctga agcaccgaat ccgctgacgt
     4321 gtatacaatc atcgaaccgt tctttatctc ttcctctccg agctcctcga ttatctcagt
     4381 gccgctggcg ctcttgttgc ctatgacctt ttttccgcac tgcttctcaa gctcagcgat
     4441 gagctccggt ggaaatccgt gctctgtgaa ggtcttaaag ggcttttcgg tttttatgcc
     4501 catcatctcc cagtggcctg tcatcgtgtc cttgccgttg cttgcctctg ccagccgcgt
     4561 aaatctgccg attggctcag cagccggctg catctgaccg ccacagtgca gattgagcat
     4621 tccaagcctt gtaagatttg ggatgtcaag agtctgcatc ctctcaagta tatgtccaaa
     4681 agtgtctaca tctgtgtctc caaacctttc cgagtccggc attgcgccga ttcccagaga
     4741 atccaggact atcacaaaaa ttctgttata tttcttcatg gtgtcatacc tccattttcc
     4801 ggtccgtaaa tttgcacagc ggggagctgt taaatctcca taagtctcac ggcactacat
     4861 cagctgccta aaaatccgct ccttcacggc atcatctgca aatgccacat ccagtgcatt
     4921 tttcatcata agcggcagct cctcttcgcg tatgccatat gttttttcta tgaactcaag
     4981 ctccttagat aaagttgtgt tgctgacagt gcggttgtct gtgtttattg tgacctttaa
     5041 tcccgcatcc aaaaactccc tgatgggata ttcgtctgca gatgccaccg cctttgtctg
     5101 caggttgctt attggacaaa gctcgatgcc tatacccttt gccgaaagct gcctttcaag
     5161 ttctccgtgg cctctcatgg ctattccatg gcctatcctg accgcgccgg cctccactgc
     5221 atctatgata ttctgtgcat ttccacactc tcccgcatgg attgtaaaag gcagcccgag
     5281 ctgctttgca tatttgaaaa gctccatgaa gcc
//
LOCUS       DPUF01000039            4520 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6269, whole
            genome shotgun sequence.
ACCESSION   DPUF01000039 DPUF01000000
VERSION     DPUF01000039.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6269"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..>552
                     /locus_tag="DHV81_01410"
     CDS             <1..>552
                     /locus_tag="DHV81_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HCI93196.1"
                     /translation="NPEYIPKLTYEAYLDFYHKYYHPSNSYIYLYGDMDVVERLEWLD
                     KEYLSLYDYKKVNSEINKQPAFDEIKNVEAQYSITMDDSQENKTYLSYNRVVGDSLDE
                     MLYQAFDVLDYALVSSPGAPVKQALIDAGIGDDVYGSYDAGILQPVFSFVAKNANASQ
                     ADEFESIIENTLKEVVKTGINKEA"
     assembly_gap    553..728
                     /estimated_length=176
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <729..2351
                     /locus_tag="DHV81_01415"
     CDS             <729..2351
                     /locus_tag="DHV81_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HCI93197.1"
                     /translation="KLRKAVDTDYFEKLIQEYLLDNTHGSSVTVKPKRGLGNEREEVL
                     AKELSDYKASLSDEEIKKLIEDTEHLKKYQEEPSSDEDLRKLPMLTRADMKKNAMPFS
                     NIEDELLDVKVVRHDIESNGIDYISFLFDAGDFAQSELGYLGFFTNALGLVSTEKYSY
                     TDLANATNIYTGGISTGTASHPDIKDRNNFVFKLEVKLKVLEKNLDKALELMEQMLLS
                     SDFTDTKRLGELVAQIKARLQANLSSSGHLVAAMRSMSSFSRYALYQDELKGVAFYRS
                     ICRIEKELSESPKSVSDKLAAIAKKLFARNRMLISFTGNNEAYGNAKPSLEKVIAGFD
                     KMSVIGNQAEVHFNTAKEAFIDASQIQYVAKTGDFICEGYEYTGALRLLRIILSYDYL
                     WINVRVKGGAYGCMNTFLRSGESYFVSYRDPNLSDTLDVYDRIPEYIKSFSPDERDMT
                     KYIIGTFSALDTPMNPEAKGSRSLSAYLEGITYEQIQKERNEILNAQPEDIRRLADLV
                     EAVLKKDSICVIGNENMIKESAGLFENVEKLI"
     gene            2376..>3130
                     /locus_tag="DHV81_01420"
     CDS             2376..>3130
                     /locus_tag="DHV81_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCI93198.1"
                     /translation="MIQNFKSGFVTIIGRPNVGKSTLMNRLIGQKIAITSNKPQTTRN
                     RIQTVYTDMEKGQIVFLDTPGIHKAKNKLGEYMVNVAEKTLNEVDVVLWLVEPTNFIG
                     AGEQHIIEQLKKVNTPVILIINKVDTVEKEKVLEYIDTYRKVYDFAEIIPTSALRGQN
                     TDDVINSIFKYLPYGPQFYDEDTITDQPERAICAEIIREKALHALNDEVPHGIAVGID
                     RMKTRKTKSGSIIDMDATIVCERDSHKGIIIGK"
     assembly_gap    3131..3236
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3332..4060
                     /gene="recO"
                     /locus_tag="DHV81_01425"
     CDS             3332..4060
                     /gene="recO"
                     /locus_tag="DHV81_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCI93199.1"
                     /translation="MSQSVVLTGMVLSAMPVGDFDKRITILTKERGKITAFARGARRP
                     NSQLMAGSNPFSFGEFELFEGKSAYSLSKVTISNYFRELTQDLEAVYTGFYFLEFCGY
                     FCQENNDEKDMLKLLYQSLRALESPAYKNELVRAVFELKAITINGEGPSLFSCIHCHS
                     REGLNVFSARRGGVFCDSCGTQVQGRHILSDTLYTMQYIVSADITKLYRFAVSDDVLE
                     ELRSIMKEYLSIYVHHDFKSAAFL"
     gene            4222..>4520
                     /locus_tag="DHV81_01430"
     CDS             4222..>4520
                     /locus_tag="DHV81_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93200.1"
                     /translation="MNKKLSMLLPVIATCGLLAGCGTDYYTKDSTVFVAKNGSVVSTD
                     VEDFDTATYKQDDLQSYVDKSIDDYNKKNDGSVKLKKLTVEKKKASLTMSYASTD"
BASE COUNT         1352 a          734 c         1033 g         1119 t
ORIGIN      
        1 aatccggagt atattcctaa gcttacgtat gaagcatatc tggacttcta tcacaagtat
       61 tatcatccat ccaattcata catttatctg tatggtgata tggatgttgt cgagagactt
      121 gagtggctgg ataaggaata tctgagcctg tatgattata aaaaagttaa ttcagaaatt
      181 aataaacagc ctgcatttga tgaaataaag aatgttgagg cgcagtattc aattaccatg
      241 gatgattcac aggagaataa gacttatcta tcttataacc gtgtggtagg ggattcgctt
      301 gatgagatgc tctatcaggc atttgatgtg cttgactatg cacttgtcag ctcaccggga
      361 gcacctgtga agcaggcatt gatagatgcg ggaatcggtg atgatgtgta tggttcatat
      421 gatgcgggca ttctgcagcc ggtattctcg tttgtggcaa agaatgccaa tgcatctcag
      481 gcagatgagt ttgaaagtat tatagaaaac actcttaaag aagttgttaa aacaggaatt
      541 aataaagaag cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnaa gcttcgaaag gctgtagata cggattattt tgaaaagctt atacaggagt
      781 atctgcttga caatacccat ggttcatctg tcacagtaaa gccgaagcgt ggacttggta
      841 atgagaggga ggaggttttg gcaaaggagc tttccgatta caaggcatct ctttcagatg
      901 aagagattaa aaagctgatt gaggatacag agcatttaaa gaaataccag gaggagcctt
      961 cgtcggatga ggatctgcgc aagcttccaa tgcttacaag agcagatatg aaaaagaatg
     1021 ctatgccttt ttcaaacatt gaggatgaac ttttggatgt aaaggttgtg cgtcatgaca
     1081 tagagtcaaa cggcattgat tatatatcat ttttatttga tgcaggtgat tttgcccaga
     1141 gtgagcttgg ctatcttggc tttttcacaa acgcactcgg gctggtgagc accgaaaagt
     1201 acagctatac cgatttagcc aatgccacca acatatatac cggcggaatc agcacaggta
     1261 ctgcaagtca cccggatatt aaggatagaa ataatttcgt gtttaagtta gaggtgaagc
     1321 ttaaggtgtt ggagaaaaat cttgacaagg cacttgagct tatggagcag atgcttcttt
     1381 catcggattt tacagataca aagagactgg gtgagcttgt agcgcagatt aaggcaaggc
     1441 ttcaggctaa ccttagcagt tccggtcatc tggttgcagc tatgcgaagt atgtcgagct
     1501 tctcgcgcta cgcactttat caggacgagc taaagggagt tgctttttac cgctctatat
     1561 gccgcataga aaaggaactt tcagagtcac caaagagtgt gtcagataag cttgctgcaa
     1621 tagccaaaaa gctgtttgca agaaacagaa tgcttatcag ctttaccgga aataacgagg
     1681 catatggcaa tgcaaagcct tcgttggaaa aagttatagc tggatttgat aaaatgagtg
     1741 ttataggcaa tcaggcagag gttcatttta atacagcaaa ggaagcattt attgatgcat
     1801 cccagataca gtatgtggca aaaacaggtg atttcatctg cgagggctat gaatacaccg
     1861 gagcactgag actgctgcgt atcatcttaa gctatgatta tctctggata aatgtcaggg
     1921 taaagggtgg tgcttacggc tgcatgaata cattccttag aagcggagag agctactttg
     1981 tctcatatag agaccctaac ctttcagaca cacttgatgt ctatgacaga atacctgagt
     2041 atataaagag cttttcgcct gatgagaggg atatgaccaa gtatattatc ggtaccttca
     2101 gtgctcttga tacgcctatg aatccggaag caaagggaag cagatcactg tcggcatatc
     2161 tcgagggtat tacttacgag cagattcaaa aggagagaaa tgagatacta aatgcacagc
     2221 cagaggatat cagaaggctt gcagaccttg tagaggcagt gcttaagaag gacagtatat
     2281 gtgtcatcgg caatgaaaat atgattaaag agtcggcagg gctctttgag aatgttgaaa
     2341 agcttatata aatacagatt cacataggag gaaaaatgat acagaatttt aaatcaggtt
     2401 ttgttacaat aatcggcaga ccaaacgtgg gcaagtcaac actgatgaac aggcttatcg
     2461 gtcagaaaat tgccatcaca tcaaacaagc ctcagaccac aagaaaccgc atccagacag
     2521 tatacacgga tatggaaaag ggacagatag tgttccttga tactccgggc atacacaagg
     2581 caaaaaacaa gctgggcgag tacatggtca atgtggcgga aaagacattg aatgaggtgg
     2641 atgttgtgct gtggcttgtg gagccgacaa actttatcgg ggcaggagag cagcatatca
     2701 tagagcagct caaaaaggtc aatacacctg ttatattgat tatcaacaag gtagatacag
     2761 tggaaaagga aaaggtgctt gaatatatag acacctaccg caaggtatac gactttgcag
     2821 agattattcc tacgtcagcc ttaagagggc agaatacgga tgatgttatt aattctatat
     2881 ttaagtattt gccttatggc ccacagttct atgatgagga caccatcaca gatcagccgg
     2941 agcgtgcaat ctgtgctgag attatcaggg aaaaagcact ccatgctcta aatgacgaag
     3001 tgccgcatgg cattgcagta ggaattgacc gcatgaagac aagaaagaca aagagtggtt
     3061 ctattataga tatggatgcc acaattgtct gcgagcgaga ctcccataag ggaatcatta
     3121 taggaaagca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggt
     3241 gaaggtaaag aaggattgga gagacagcga cttcctcatc aagaactttg gatacaaaga
     3301 ggaagaatag tattttgaaa gtaagattaa gatgtcacag agtgtagtgt tgaccggtat
     3361 ggtgctttca gccatgccgg taggggattt tgacaaaaga ataacgattc tcacaaagga
     3421 acgagggaaa atcacagcct ttgcaagggg cgcaagacgt ccaaacagcc agcttatggc
     3481 aggaagcaat ccattttcgt tcggtgagtt tgagctgttt gagggtaaaa gcgcatattc
     3541 tctttcaaag gtcacaatca gcaactattt cagggagctg acacaggatt tggaagcagt
     3601 atacaccggg ttttactttc tggagttctg cggatatttc tgtcaggaga acaatgacga
     3661 gaaagatatg ctaaagctcc tttatcaatc actcagggct cttgaatcac cggcatataa
     3721 gaatgagctg gtcagggcag tttttgagct taaggctatt acgataaatg gcgagggtcc
     3781 atcgctgttt tcgtgtatac actgtcattc aagggaagga cttaatgtct tttcggcaag
     3841 acgcggaggg gtgttttgcg atagctgtgg cacgcaggtg cagggcaggc atatactaag
     3901 cgatacactc tataccatgc agtatattgt ttcagcagat atcacaaagc tatacaggtt
     3961 tgcagtgtca gacgatgtgc ttgaggagct gagaagcata atgaaggaat atctttcaat
     4021 ctatgtgcat catgatttta aatcagcagc ttttctgtga taaattaaag catgaaatta
     4081 aatcatgaaa taattaatag ataccatgta tatcgttgtt taagctataa gcattactgt
     4141 aattgctctt ttaaatatga tacttgaaat catatataca aaagggtata atggcataga
     4201 ttatatttta ggagggcagc tatgaataaa aagctatcaa tgctgttgcc tgttattgcg
     4261 acatgcggtt tgcttgccgg gtgcggtact gattattata ccaaggatag cacggtgttt
     4321 gttgcaaaaa acggcagtgt agtatctacg gatgtcgagg actttgatac agctacatac
     4381 aagcaggatg acttacaaag ctatgtggac aagtcaatag acgattacaa taagaaaaat
     4441 gacggttcgg tcaagctcaa aaagctgact gtcgaaaaaa agaaggcatc gcttaccatg
     4501 agctatgctt cgactgacga
//
LOCUS       DPUF01000040            3603 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_24012, whole
            genome shotgun sequence.
ACCESSION   DPUF01000040 DPUF01000000
VERSION     DPUF01000040.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_24012"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(56..128)
                     /locus_tag="DHV81_01435"
     tRNA            complement(56..128)
                     /locus_tag="DHV81_01435"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(93..95),aa:Lys,seq:ctt)
     gene            344..1573
                     /gene="pepT"
                     /locus_tag="DHV81_01440"
     CDS             344..1573
                     /gene="pepT"
                     /locus_tag="DHV81_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCI93201.1"
                     /translation="MKVEERLLKYVGYHTTSDEASDKIPSSDREFALARELESELKEL
                     GLSKVVLTDHCYVYGLLPATAGMEHKKAVGFIAHMDTAPDFSGENVKPQLLRHYDGSD
                     VLLRGSGEYLRVSDFPELKALSGRTLITTDGTTLLGADDKAGVAEIMTALEQIIAEGT
                     PHGDIWVGFTPDEEVGKGADLFDLDYFEADFAYTVDGDYEGEVAYENFNAASAEFTIK
                     GVNVHPGEAKDIMVNAALVGCEIAAALPANETPATTEGREGFYHLCDMSGDVASAHLS
                     YIVRDHDKALFAKRLDTLRSLEIEMNKKYGEGTVSLTITHSYENMLSIIMDNMYIIDL
                     AKAAIEENGLEPLSRPVRGGTDGARLSFMGLPCPNLGTGGYGFHGPFEHISVEGMETA
                     VNIIKSIVKHVLMLADA"
     gene            complement(1913..3505)
                     /locus_tag="DHV81_01445"
     CDS             complement(1913..3505)
                     /locus_tag="DHV81_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCI93202.1"
                     /translation="METIRFDELELNPKILRGIKDMGFEEATPIQAQGIPAVLSGRDV
                     IGQAQTGTGKTAAFGIPVLESVDASSHKTQVIILSPTRELAIQVADEIRKLAKYMHGV
                     KVLPVYGGQDISRQIKALKGGVQIIIGTPGRLMDHLRRKTIRPDHVKTIVLDEADEML
                     NMGFREDIETVLEYLPQEHQTVLFSATMPKPILEITRKYQHDAINIKIVKKELTVANI
                     DQYYYDVKRKDKIDVLTRLLDYYNPKLSLVFCNTKKMVDELAYELCGRGYSAEGLHGD
                     MKQVQRDRVMKNFRNGKTDILIATDVAARGIDVDDVEAVFNYDLPQDDEYYVHRIGRT
                     GRAGRCGKAFSFVKGKEVYKLKDIQRYCKTKIYAQPIPSSDDVAKIRAEKIMDQISTI
                     IDEENLTSVIDIIENQINESDYTAMDIAAAFLYQAMGASEISTAESKDDYDFHNTGAE
                     DGMVRLFINVGKKDKIKPGDILGAIAGESGMPGRLVGAIDMYDKYTFVEVPAEYGKEV
                     LNAMKNAKIKGRSVNMEPANSR"
BASE COUNT          929 a          841 c          769 g         1064 t
ORIGIN      
        1 aataaaaaag caccaaccct atggctcata cgtccataaa accggtacct ttgagtgcgc
       61 ctccaggggc tcgaaccctg cacaccctga ttaagagtca ggtgctctac caaatgagct
      121 agaagcgcgt ttattaattt gtttgcgttt tccttaacgc gaaatttatt atatagtata
      181 gtttatagaa atgcaacact ttttttaatt tttttaaatt tttttttaga ttttttgtaa
      241 aacagttgat tttacaccgt tttaagctat attattttct atatacagag actttttatt
      301 ccagatttcc aatccaaagt ctctcagaaa ggatttactg ataatgaaag tagaagaaag
      361 attattaaaa tatgtgggct atcacaccac atctgatgaa gcaagtgata aaataccaag
      421 cagtgacagg gaatttgcac tcgcaagaga gcttgagtca gagcttaagg agcttggctt
      481 atccaaggtt gtgctcactg accattgtta cgtgtatggt ctgctgcctg ccactgccgg
      541 catggagcac aaaaaagcgg tcggatttat tgcacatatg gatactgcac ctgatttttc
      601 cggggaaaat gtcaagccac agttgctgcg tcactatgac ggaagcgatg tgctgcttag
      661 aggcagtggc gaatacctga gagtaagcga ttttcctgag cttaaagccc tctccggacg
      721 cactctcatt accacagatg gcactaccct gcttggtgcc gatgacaagg ccggtgtggc
      781 tgagattatg accgccttag agcagattat cgcagagggc actcctcacg gcgatatctg
      841 ggttggattt acacccgatg aagaggtcgg aaaaggtgcg gatttgtttg atctggacta
      901 tttcgaggca gattttgcct acaccgtgga cggtgattac gagggcgagg tggcctatga
      961 aaactttaat gccgcaagcg ctgaatttac cataaagggc gtcaacgtgc atccgggcga
     1021 agcaaaggac attatggtca atgctgcttt ggttggctgc gagattgccg cagcgctgcc
     1081 tgcaaacgag actcctgcca caactgaggg cagagagggc ttttaccatc tgtgtgatat
     1141 gagcggtgat gtagcatcag cccatctgtc atatattgtg cgcgaccacg acaaggctct
     1201 ttttgctaaa agacttgata ctctccgctc tcttgagata gagatgaaca aaaagtatgg
     1261 tgagggcact gtttctttga ctatcaccca ctcctacgag aatatgcttt caatcataat
     1321 ggacaacatg tacattatcg acctggctaa ggctgctata gaggaaaacg ggctagagcc
     1381 gctatcacgc ccggttcgcg gcggtacaga cggcgcacgt ttatccttca tgggacttcc
     1441 ttgtccaaac ctcggtaccg gcggctacgg cttccacggc ccatttgagc acatatcagt
     1501 ggagggcatg gagactgcgg ttaatataat caaatcaatt gtgaagcatg tgctgatgct
     1561 tgctgacgcg tgattttgct gccggtcagt tactattcac aactaacagt gtgactagtc
     1621 acggccacgt tactgttcac aactaacagt gtaaccagta atccggcagc acttatcatt
     1681 tttgcagttg ttggtggaat atttgtaaat tatcttcgca gaaatcaaaa tgatatcggc
     1741 ggaggaatgg gaaaggatta catgaaagag cattggggaa tcgatgacga caagtaatca
     1801 aaaataccgg gcaacacatt ttgtgtcccg gtatttttat acaaccatcg taactattca
     1861 gaaccactga gaacaagaaa agctgatgcg ttatcagaaa agcctgactt tgctatcgtg
     1921 aatttgcagg ctccatgttc acagagcggc cctttatctt tgcattcttc atagcattga
     1981 gcacttcctt gccatactca gccggcacct caacaaatgt atacttgtcg tacatgtcaa
     2041 ttgcaccgac aagcctgcca ggcatgcctg actcgcctgc aatggcgcct aaaatgtcac
     2101 ccggctttat cttgtccttc tttcctacgt tgataaacag acgtaccatg ccatcctctg
     2161 ctcctgtatt gtggaaatca tagtcgtcct ttgattcagc tgtggatatc tctgaagcac
     2221 ccatagcctg gtataaaaat gccgccgcta tatccattgc cgtgtaatct gactcattta
     2281 tctgattctc tataatatca atgacacttg tgagattttc ctcatctata atggtggaaa
     2341 tctggtccat aatcttttct gccctgattt tggcaacatc atcagatgat ggaattggct
     2401 gtgcatagat ttttgtcttg cagtagcgct gtatgtcctt gagcttatat acctccttgc
     2461 cctttacaaa ggagaatgcc tttccacagc ggcctgctct tcctgtacgg ccgattctat
     2521 gtacataata ctcgtcatcc tgaggcagat cataattaaa aacagcctcc acatcatcca
     2581 catcaattcc gcgcgctgcg acatcagtgg caataagaat atctgtcttt ccgtttctga
     2641 agttcttcat gacacgatct ctctgcacct gcttcatatc gccatgaagt ccttctgcac
     2701 tgtagccacg tccgcaaagc tcatatgcca gctcgtcgac catcttcttg gtgttgcaga
     2761 atacaagtga gagctttgga ttatagtaat ccaacagtct tgtgagtaca tctatcttgt
     2821 ccttgcgctt cacatcatag taatactggt cgatatttgc gactgtcagc tcttttttca
     2881 caatcttgat attgatagca tcatgttgat attttcttgt aatttccaaa ataggctttg
     2941 gcattgttgc cgagaagagt actgtctgat gctcctgtgg caggtattcg agcactgtct
     3001 cgatatcctc cctaaagccc atgttgagca tctcgtctgc ctcgtcgagc actattgtct
     3061 ttacatggtc aggcctgatt gtctttctcc tgagatgatc cataagacgc cccggggttc
     3121 cgataataat ctgcacaccg cccttaagtg ccttaatctg gcggctgata tcctgtcctc
     3181 cgtataccgg aagtaccttc acaccatgca tatattttgc aagcttcctg atttcatctg
     3241 ccacctggat tgcaagctcc cttgtaggag agaggatgat aacctgtgtc ttgtgacttg
     3301 atgcatcaac gctctcaagc accggaatac caaaggctgc tgtctttcct gttccggtct
     3361 gcgcctgtcc gattacatcc ctgcccgaaa gcactgccgg gattccctga gcctgtattg
     3421 gagttgcctc ctcaaagccc atatccttga tgcctcgcaa aatcttaggg tttagttcta
     3481 attcgtcaaa tctgattgtt tccatatatt ttttatattt cctttcaaaa aaataaagtg
     3541 tgcatatttt tataacatgc acactttaac atatttgtga gataattaca aaatattatt
     3601 tct
//
LOCUS       DPUF01000041            2536 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7948, whole
            genome shotgun sequence.
ACCESSION   DPUF01000041 DPUF01000000
VERSION     DPUF01000041.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7948"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="DHV81_01450"
     CDS             complement(<1..1392)
                     /locus_tag="DHV81_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCI93203.1"
                     /translation="MKKTEKQNTGKLKIIPLGGLEQIGMNITAFEYEDSIIVVDCGLS
                     FPEDDMLGIDLVIPDITYLKENIDKVKGFFITHGHEDHIGAIPYVLKEINVPIYATKL
                     TIGIIDRKLEEHGMLKTVKRKVVKYGQHINLGCFRVEFIKTNHSIQDAAALAIYSPAG
                     IVVHTGDFKVDYTPVFGDAIDLARFGEIGKKGVLALMCDSTNAERPGFTQSEKSVGKV
                     LDGIFEDHRKNRIIIATFASNVDRVQQIINCAEKYGRKVAIEGRSMVNIISIASELGY
                     LSLPDNILIDVEQLRSYPDEQTVIITTGSQGESMAALSRMANGTHRKVSIKPNDTIVF
                     SSNPIPGNEKSVTGIINELMMKGADVIFQDVHVSGHACQEDIKLIYSLVNPKYAIPVH
                     GEYKHLVAQAKIAEQLGYDTDHIKILSSGDVLEINEDGAKVTGRVHVGNVMVDGLGGG
                     DVGNNVLRDRQRLA"
     gene            complement(1432..1695)
                     /locus_tag="DHV81_01455"
     CDS             complement(1432..1695)
                     /locus_tag="DHV81_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCI93204.1"
                     /translation="MQKIKFTDPQTNDVVEFAVEEETTLNGTRYLLVSEGEESGDCEA
                     YILKEVSTKDEEVLYQMVEDDVEFSAIAKVFSELTDDDTALEY"
     gene            complement(1701..2132)
                     /locus_tag="DHV81_01460"
     CDS             complement(1701..2132)
                     /locus_tag="DHV81_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCI93205.1"
                     /translation="MRILGLDFGSKTVGVAVSDELLITAQGVEIVRRKSPSKLRQTLA
                     RIEEIIAEYKVDRIVLGYPKNMNNTEGERCEKTKEFGDMLARRTGLEVIYWDERLTTV
                     AADRSMMETGIRRENRKEFVDEIAAIFILQGYLDYLSNSRS"
     gene            complement(2134..2400)
                     /locus_tag="DHV81_01465"
     CDS             complement(2134..2400)
                     /locus_tag="DHV81_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCI93206.1"
                     /translation="MEKNINNTQFFTVQKEPELQVSDVLEIVYKALKEKGYNPVNQIV
                     GYIMSGDPTYITSHNNARSLIMKVERDELVEEVLKTYIENSAWD"
BASE COUNT          656 a          643 c          439 g          798 t
ORIGIN      
        1 agccagacgc tgtctgtcac ggagcacgtt atttcctaca tctcctccac caagtccatc
       61 gaccatgaca ttgcccacat gcactctgcc tgtcaccttg gcaccgtcct cattgatttc
      121 aagcacatct cctgatgaaa ggatcttgat gtggtctgta tcatatccga gctgttcggc
      181 tattttggcc tgtgccacaa gatgcttgta ctctccatga acagggattg cgtactttgg
      241 attgacaaga gaataaataa gctttatatc ctcctggcac gcatgaccgg aaacatggac
      301 atcctggaat ataacgtctg cccccttcat catgagctca ttgatgatac ctgtaaccga
      361 cttctcattt cccggtatag ggtttgagct gaatacgatg gtatcgtttg gctttatgga
      421 tacctttctg tgggtaccgt ttgccatacg tgaaagagct gccattgact ctccctggct
      481 tcctgtcgtg atgatgacag tctgctcatc aggatagctt ctaagctgtt ctacatctat
      541 aagaatattg tcaggcagcg acagatagcc aagctctgag gctatcgaaa tgatatttac
      601 catgcttcgt ccctcgatgg ccacctttct gccatacttc tccgcacagt tgataatctg
      661 ctgcacacgg tctacatttg atgcaaatgt agcaatgata attctgtttt tcctgtgatc
      721 ctcaaagata ccgtccaata cctttccgac agacttctca gactgtgtga agcccggtct
      781 ttctgcattg gtactgtcgc acataagcgc gagcacgccc ttctttccaa tctcaccaaa
      841 ccttgcaagg tcgattgcat ctccaaatac aggtgtataa tccaccttga aatcgcctgt
      901 atgaacaact atacctgccg gtgaatagat agcaagcgct gccgcatcct ggatactgtg
      961 gttagtcttt ataaactcaa ccctgaagca gccaaggttg atatgctgtc cgtattttac
     1021 gacttttctc ttgacagtct tgagcatgcc atgctcttcg agctttctgt caataattcc
     1081 gatggtaagc tttgtagcgt atatcggtac atttatctcc ttaagcacat aaggaatagc
     1141 accgatatga tcctcatgtc catgagtgat aaagaagccc ttgaccttat cgatgttctc
     1201 cttaagatat gtgatatcag ggataaccaa atctattccc aacatatcgt cctcagggaa
     1261 tgataatccg caatctacta caataatact gtcctcatac tcaaaggcag taatattcat
     1321 tccaatctgc tctaatcctc caagtggaat gatcttaagc ttacctgtat tctgtttttc
     1381 tgttttcttc aaaatattac ctccaaattt atttcacgta caactgcaat cctaatattc
     1441 aagtgcagtg tcatcatccg taagctcaga aaatacctta gcaatagccg aaaactcaac
     1501 atcgtcttca accatctgat aaagcacctc ttcatccttt gtgcttactt cttttaaaat
     1561 atatgcttca cagtctccgc tttcttctcc ttcggaaact aaaagatatc gtgttccatt
     1621 aagtgttgtc tcttcttcaa ccgcaaactc gacaacatca ttagtctgcg ggtcagtaaa
     1681 tttaattttc tgcataaaaa ctaagaccga ctatttgaca gataatccaa atatccctgc
     1741 aaaataaaaa tcgctgctat ctcatcaaca aactccttgc gattctccct gcgtatgccg
     1801 gtttccatca tagaacggtc ggctgcgact gttgtaagac gctcatccca gtatatgacc
     1861 tcaagacctg tgcgccttgc aagcatatca ccgaactcct tcgtcttctc gcagcgttct
     1921 ccttccgtgt tattcatgtt tttaggatag ccgagcacaa tcctgtccac cttgtactct
     1981 gcaataatct cctcaatacg tgccaaagtc tgtctgagct tggatggcga cttcctgcgc
     2041 actatctcaa caccctgtgc tgtgattaag agctcgtcgc tcaccgctac tccgactgtc
     2101 tttgaaccaa aatcaagacc taatatcctc atattaatcc catgcgctgt tctcaatgta
     2161 tgtcttgaga acctcctcca caagctcgtc tctctcaacc ttcatgatga gacttctcgc
     2221 gttattgtgg cttgtgatat atgttggatc tccggacata atatatccaa caatctgatt
     2281 aacaggattg tagccctttt cctttaatgc cttgtacaca atctcaagta catcgcttac
     2341 ctgaagctca ggctcctttt gaactgtaaa aaattgtgtg ttgttaatat ttttttccat
     2401 acgtttcgcc cttcttgtaa tattatttta ttataaatgt aataaaatta caacctttta
     2461 ttattcgaag agtaatattt cgtctgtaag catacccgat atacggcctg aaactatctg
     2521 attttcaagg ttttcg
//
LOCUS       DPUF01000042           12161 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3468, whole
            genome shotgun sequence.
ACCESSION   DPUF01000042 DPUF01000000
VERSION     DPUF01000042.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 12161)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12161)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12161
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3468"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="DHV81_01470"
     CDS             complement(<1..692)
                     /locus_tag="DHV81_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCI93207.1"
                     /translation="MPETNNIENNQAMQFDAIQIGLASPEKIREWSHGEVKKPETINY
                     RTLKPEKDGLFCEKIFGPTKDWECHCGKYKKIRYKGVVCDRCGVEITKSSVRRERMGH
                     IELAAPVSHIWYFKGIPSRMGLILDLSPRVLERVLYFASYIVLDPGETKLAYKQILNE
                     SEYQEACDTYGRSAFRVGMGAESIHELLAAIDLEKDSAELKAELENATGQKRARIIKR
                     LEVVEAFRESGN"
     gene            complement(711..4673)
                     /locus_tag="DHV81_01475"
                     /pseudo
     CDS             complement(711..4673)
                     /locus_tag="DHV81_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515517.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
     assembly_gap    1158..1182
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4890..5077
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5078..5442)
                     /locus_tag="DHV81_01480"
     CDS             complement(<5078..5442)
                     /locus_tag="DHV81_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCI93208.1"
                     /translation="MAKLTTAEFIDAIKELSVLELNDLVKACEEEFGVSAAAGVVVAA
                     AGADGAAAEEKTEFDVELTEVGPNKVKVIKVVREITGLGLKEAKDAVDGAPKVIKEQL
                     DKASAEDAKAKLEAEGAKVT"
     gene            complement(5503..6054)
                     /locus_tag="DHV81_01485"
     CDS             complement(5503..6054)
                     /locus_tag="DHV81_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCI93209.1"
                     /translation="MAKVELKQPIVDEISGVINGAQSVVVVDYLGLTVAEDTQLRKQL
                     REAGVTYKVYKNTLVNRAIQGTEFEGLKDVLEGPSAFAVSAEDATAPARILAKFAKTA
                     PALEIKAGVVEGTFYDAEGMKAIAAVPSRDELLGKLLGSIQSPITNLARVLNQIAEQG
                     GAADAAVEAAPAEDAAEEAPAAE"
     gene            complement(6345..7040)
                     /locus_tag="DHV81_01490"
     CDS             complement(6345..7040)
                     /locus_tag="DHV81_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCI93210.1"
                     /translation="MKRGKKYQDAVKAFDKKEQYDVADAISIVKKNATAKFDETIELH
                     LRTGCDGRHAEQQIRGAVVLPHGTGKTVKVLVFAKGTKVDEAQAAGADFVGGEELIPK
                     IQNEGWLDFDVVVATPDMMGVVGRLGRVLGPKGLMPNPKAGTVTMDVTKAVNDIKAGK
                     IEYRLDKTNIIHVPVGKASFTDEQLNDNFQSLMGAINKAKPASLKGQYIKSATLTSTM
                     GPGVKLNVVKITQ"
     assembly_gap    7106..7200
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7201..7613)
                     /gene="rplK"
                     /locus_tag="DHV81_01495"
     CDS             complement(<7201..7613)
                     /gene="rplK"
                     /locus_tag="DHV81_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCI93211.1"
                     /translation="MAKKVEGYIKLQIPAGKATPAPPVGPALGQHGVNIVEFTKQFNA
                     KTADQGDLIIPVVITVYADRSFSFITKTPPAPVLIKKACKIQSGSGEPNKKKVAKITK
                     AQVQEIAELKMPDLNAASLESAMSMIAGTARSMGV"
     gene            complement(7628..8146)
                     /gene="nusG"
                     /locus_tag="DHV81_01500"
     CDS             complement(7628..8146)
                     /gene="nusG"
                     /locus_tag="DHV81_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HCI93212.1"
                     /translation="MTEANWYVVHTYSGYENKVKANIDKTIENRHLEDQILEVRVPLE
                     DVTEVKNGVRKQVSKKMFPGYVLIHMIMNDDTWYVVRNTRGVTGFVGPGSKPVPLTEA
                     EMRPLGIKTENVVVDFAEGDMIVVIGGVWKDTVGVITAMNYHKQTVTINVELFGRETP
                     VEVSFADVKKTN"
     gene            complement(8165..8389)
                     /locus_tag="DHV81_01505"
                     /pseudo
     CDS             complement(8165..8389)
                     /locus_tag="DHV81_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741284.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
     assembly_gap    8266..8354
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8417..8566)
                     /gene="rpmG"
                     /locus_tag="DHV81_01510"
     CDS             complement(8417..8566)
                     /gene="rpmG"
                     /locus_tag="DHV81_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCI93213.1"
                     /translation="MRTKITLACTECQRRNYNMTKDKKTHPDRMETKKYCRFCRTHTM
                     HKETK"
     gene            9009..10310
                     /locus_tag="DHV81_01515"
     CDS             9009..10310
                     /locus_tag="DHV81_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar capping protein"
                     /protein_id="HCI93214.1"
                     /translation="MAKIDSAYAYYASTYANKEVSRYDSHKKSDLRKIYNRIIKTNKE
                     SPLYKISNMSEAKKYAIDIKESSKAILNAVDSLSDKYAGSSDSFQKKVAESSNEDAVG
                     VRYVGDGSENKGADGFDIDVRQLAAPQINTGSYLDSKALSFIPGSYSFDINTNASSYE
                     FQFGVSTGETNGEVQDKLKRLINASGLGIEADIVHDDSGKAALQLTSMQTGLSETEDS
                     LFSVAPSANAESISMMKQLGIDKVSSPAHNSDFSLNGTAHSSLSNTFTINNTFELTLK
                     KPTTDTGAAAIGFKANSDAVADNVQTLVDAYNKMIDVADDYSVNDSADATRLLRDISS
                     ITKGSQSKLSYIGLMTDDDGKLTIDRDILAGALSPDRADDTFNTLTALKDAIGDKAKS
                     VTVNPMNYVQKVVVAYKNPGHNFNTPYISSIYSGMMLDSYA"
     gene            complement(10357..10803)
                     /locus_tag="DHV81_01520"
     CDS             complement(10357..10803)
                     /locus_tag="DHV81_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93215.1"
                     /translation="MSRNIENHDSVLLLQDCIAGCKMALESMDDIREHTDDKQLRELI
                     DKYYDEHTGLSVDCRDMLDKAGADIKEPGMFAKMAAKLQTAMKMAVNDDQSQAAKLLT
                     EGCNMGIETISGSLNEYRDADSKSVAIAERLRTLEEKMVGDLQPLL"
     gene            complement(10860..12095)
                     /locus_tag="DHV81_01525"
     CDS             complement(10860..12095)
                     /locus_tag="DHV81_01525"
                     /inference="COORDINATES: protein motif:HMM:PF14501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93216.1"
                     /translation="MHAYTQNGEEDTVLRDDLKRVKKKLSLTFAIAIIGTCVVFGLLY
                     YLGIDLLNNYIEGTSHLIYYNEKPYIDEFQQYVSDNDISTTDVEQYDEWMSEHDVSFY
                     SIQEEGKVIYNKSNVDKLISPAKNMYDYRFTGSYELPVQFSDIEAKVFIYTGFTIKYY
                     ITLMIFDVGFSLMMGIVFVYLRIRKIIAGYQIELEASQEQEKKARQEKDELMRNMAHD
                     LRTPLTGVMTYVDVMKLENEAYAENMKNLNFISEKVLDIRTQVDNLLDFSIAGSQRPV
                     ELDASMDVEYIFGDYLSDVCAQLMKSNYEVDAEGISFKQVKVAVNMAFLTRIFSNLTD
                     NIYKYADNKKPVVMKTAFTKKTFSVEIGNGVCDNKTLLKSAGIGLKSVDAMMQRMNGS
                     MSFKREHGKFWVKLEFPIV"
     gene            complement(12046..>12161)
                     /locus_tag="DHV81_01530"
     CDS             complement(12046..>12161)
                     /locus_tag="DHV81_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93217.1"
                     /translation="CYGYIIVHIKNLRNKLIKACGDACIHTEWGRGYRFER"
BASE COUNT         3148 a         2886 c         2149 g         3581 t
ORIGIN      
        1 ttgtttcctg actcacggaa agcctctaca acctcaagtc tcttgatgat acgtgcacgc
       61 ttctggcctg tagcattctc aagctcagcc ttaagctctg ccgaatcctt ctcgagatcg
      121 attgctgcta aaagctcatg gatagactca gcacccattc ctacacggaa tgctgagcgg
      181 ccataagtat cgcatgcctc ctggtactct gactcgttga gaatctgctt gtatgcaagc
      241 tttgtctcgc ccgggtcaag cacaatatat gaagcaaagt acagcactct ctcaagtaca
      301 cgtggagata agtcaaggat gagtcccata cgtgatggga tacccttgaa ataccagata
      361 tgagatacag gagcagcaag ctcgatgtgt cccatacgct ctctacgaac gcttgacttt
      421 gtgatttcta cgccacatcg gtcgcagaca acacccttat aacgtatctt cttgtattta
      481 ccacaatgac actcccagtc cttcgttggt ccgaaaatct tctcgcagaa aagaccgtct
      541 ttttcaggct ttaatgttct atagttaata gtctcaggct ttttaacctc gccatgtgac
      601 cactctctga tcttttcagg agatgctaat ccaatctgaa tcgcatcaaa ctgcattgcc
      661 tgattatttt ctatattatt tgtttctggc attttggcac tcccttctaa ttattcttcg
      721 tcatcaagaa cttcatcagc aaaaatatcg tcatccgctg tctcagggga ctcttcctct
      781 tctatgtcaa caagctcttc gttgtcatca aattctttct ttgagaaacc atgctctgcg
      841 aatgactcac tgtcctcgaa tgcatagtcc ttatcgtttc cgataattga gttgatatct
      901 gtattaccgt actcacttgt ctcgataagc tcaacctctt ctctgttctc atcaagtacc
      961 ttgacatcca gaccaagtgc ctgaagctct ttgagaagta ccttgaatga ctctggaata
     1021 ccaggctcag ggatattctc acccttgatg atagcctcgt aggtcttgac acggccaaca
     1081 acatcatcgg acttcattgt aaggatctcc tgaagtgtat atgcagcacc atatgcctca
     1141 agagcccaaa cctccatnnn nnnnnnnnnn nnnnnnnnnn nnccgaaacg ctgtccaccg
     1201 aactgtgcct taccaccgag tggctgctgt gttactaatg agtaaggacc tgttgaacgt
     1261 gcatggatct tatcgtcaac aagatgatgc agcttaaggt aatgcatgtg tccgatagtt
     1321 gtctttccat cgaagcactc acctgttcta ccatcacgaa gctgtacctt tccatccttt
     1381 gaaatcggaa cgcccttcca taagcttctg tgagctctgt ttgttgaaag gtattccata
     1441 acgtcctcac ggagtgtatc aacatattta tcatagaaga tttcagcatc tgcgctccag
     1501 tatggatgct cagcctgtaa atccttttca agctcttcct tatcgaacgg aagatttacg
     1561 taatcatttg caagctcaag tgtatcctcg atatctacct cgttagctcc gtcaaataca
     1621 ggagttgaga tattgaagcc aagtgccttt gcagcgagac tcaggtgaat ctcaaggacc
     1681 tgaccgatgt tcattcgtga aggaacaccg agtgggttaa gtacgatatc cagtggacgt
     1741 ccgtttggaa ggaatggcat atcctcaaca ggaagcacac gggaaacgac acccttgtta
     1801 ccgtgacgtc cggccatctt atcaccgaca gaaatctttc tcttctgtgc aatgtagata
     1861 cgtactgact gatttactcc cggagaaagc tcatcgccgt tctctcttgt aaataccttg
     1921 gcatcaacaa tgataccgta agcaccatga ggtaccttaa gtgatgtatc acgaacctct
     1981 cttgccttct caccgaagat ggcacgaaga agtctctcct ctgctgtaag ctctgtctca
     2041 ccctttggtg taacctttcc tacaaggata tcaccggcac gaacctctgc acctacacgg
     2101 atgatacctc tctcatcaag gtccttgagt gcgtcgtcgc cgacaccagg gacatcacgt
     2161 gtaatctcct caggtccgag ctttgtatca cgtgactcta cttcgtattc ctcgatatga
     2221 actgatgtat acacatcata ctctacgaga cgctctgaaa gaagaacggc atcctcgtag
     2281 ttgtatcctt cccatgtcat gaatccgata agcgggttct ttccaagagc aagctctccc
     2341 tgacttgttg aaggaccgtc tgcgataaca tcgcctgcct taaccttgtc acccttaaat
     2401 acgataggtc tctggttgta gcagtttgac tggttagaac gagagaactt tgtaaggata
     2461 tactctctct tgtttccgtc agtctctttt atgataatgc ggtttgactc tgaagataat
     2521 acctctccgt ctgcctttgc aactacgcag acacctgagt caactgcggc ctttgtctca
     2581 atacctgtac caactacagg agcctctgtg gtcagaagcg gaacggcctg acgctgcatg
     2641 tttgatccca tgagggcacg tgtagggtca tcgttctgca agaacggaat cagcgctgta
     2701 gctactgaga ataccatctt tggagataca tccatataat caaatgctga cttatcgtac
     2761 tcctgagttt cctctctgta acgacctgaa actgagttac gtatgaaatg tccttcggca
     2821 tcaagcttct cattagcctg tgctacgtgg tagttgtcct cctcgtcggc tgtcatgtat
     2881 acaacctctt ctgtgactac cgggttctta ggatcagtct tgtcaatctt acggtatgga
     2941 gcctctacga agccatactc attgattctc gcatagcatg caagtgagtt gataagaccg
     3001 atgtttggtc cctcaggagt ctctacagga cacatacggc cgtagtgagt gtaatgtaca
     3061 tctcgaacct cgaatccggc acggtctctt gaaagaccac caggtccaag tgctgataaa
     3121 cgtctcttgt gagtaagctc tccaagaggg ttgttctgat ccatgaactg tgaaagctgt
     3181 gaagaaccga agaattcttt aacagcggca gttacaggct ttatattgat aagtgactga
     3241 ggtgaaattc cctctaagtc ctgtgtagtc attctttcac gaacaacacg ctccagtctt
     3301 gagagaccga tacggtactg gttctgtaac agctctccaa ccgcacggat acgtctgttt
     3361 ccaagatgat cgatatcgtc atctgttcct gcgccccact ctaagtgcat attgtagttg
     3421 atagttgcga agatatcctc tttagtaata tgctttggaa taaggtcaga tatatctctc
     3481 tttatagcct cgattctgtc ctcaagagaa tcatactcct cgagaatctg agcgagtacc
     3541 ggatagtaaa caagctcagt aacaccgata gacttaggat cctcaagctc aggaatccag
     3601 cttctgatgt caaccatcat agaagaaaga atcttgacat ttcttgtctc tgtctgaatc
     3661 cataaagcag gaacggcagc gttctggatc tgtgtagcaa gcgcacggtc aacaacagta
     3721 cctgcctcag cgataacctc acctgtagaa ggatccacta cgtcctcagc taaaacctgt
     3781 ccactaatac ggttctttaa agcaagcttc ttattaaact tataacgtcc taccttggca
     3841 aggtcatatc ttctaggatc gaagaacatt gctgtgataa ggctctctgc actctcaact
     3901 gaaagcggct cgcccggacg aatcttctta tataactcta ataaaccagc ctcatagctt
     3961 ccctgtggct cacgctctgt gattgcaggg tccttgctga atgatgcctg gatctttggc
     4021 tcatctccga acatatcgag aatagcctgg tttgtaccta ctccaagtgc acgtaaaagt
     4081 actgttatag gtaccttacg tgttctatct actcttacat aaaaaacgtc gttagagtct
     4141 gtctcatact caagccatgc accacggttt ggaataacag tacaagaata cagggtcttt
     4201 ccgagcttat catgagcaat tccgtaataa ataccaggag aacgaactaa ctgactgaca
     4261 ataactcgct ctgcaccgtt gattacgaat gtaccggtct ctgtcattaa aggcagatca
     4321 cccatgaaaa tttcatgctc attaatctca tctgtctctt tgttgtgaag tctgacttta
     4381 acctttaaag gagctgcgta tgttgcatct ctttccttgc actcaggaat tgtatacttt
     4441 acatcatctt cgcataatgt aaaatctaca aaatcaagac taagctttcc gctgtaatct
     4501 gtgattggag agatatccgc aaaagcttcc ttgagacctt cgtctaagaa ccactgataa
     4561 gaatccttct ggacctcaat cagatttggc atctgcagaa cctctttttg tcttgagtaa
     4621 ctcatacgca tgctttttcc ggatgtgacc ggacgtattc tgtttttctc cattgacgtt
     4681 tcacctctcg tttttatttt aatctagcaa gtatctagat aatgattcaa aaaccacccc
     4741 agttactata tcttgtggtt tttctcttgt gcacatattt gcgaatgcat tttagtgtaa
     4801 tttacctatt gacaaatttg catcatttaa aaaattgcat aaaaatgcca ctgcaccaca
     4861 atagtgcaac gtatactata caacaaatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta accttagcac cctctgcctc
     5101 aagtttagcc ttagcatcct ctgctgaagc cttgtcaagc tgctctttga taacctttgg
     5161 tgcgccatca acagcgtcct tagcctcttt aagtccaaga cctgtgatct cacgaacaac
     5221 cttgataacc ttaaccttgt ttggtccaac ctctgtaagc tctacgtcga actcagtctt
     5281 ctcctcagct gctgctccgt cagctcctgc tgctgcaact acaacacctg ctgctgcaga
     5341 aacaccgaac tcttcttcac aagcttttac taaatcattt aactctaata cgcttaactc
     5401 tttgatagca tcaataaact ctgctgttgt taactttgcc attataattt cctccattta
     5461 aaaatttaat tatgataata aggttcaaat ataatatttg aattattctg ctgctggagc
     5521 ttcctctgca gcatcctctg ctggtgctgc ctctacggct gcatctgcag cgccaccctg
     5581 ctctgcgatc tgattgagta cacgagcaag atttgtgata ggtgactgaa tgcttccaag
     5641 taattttcca agaagctcat ctctgctagg tacagcggcg attgccttca taccctcagc
     5701 atcataaaat gttccctcaa ctacacctgc tttaatctct aaagccggag ctgttttagc
     5761 gaacttagct aagattctag ctggtgctgt tgcatcctct gctgatacag cgaatgcgct
     5821 tggtccttca aggacatcct ttaatccctc gaactctgtt ccctggattg cacggtttac
     5881 taaagtattc ttgtatacct tgtaagttac accggcttct cttaactgtt tacgtaactg
     5941 agtatcctct gctactgtga gtcctaagta atcaacaact actactgact gtgcaccatt
     6001 gataacaccg gaaatctcat ctacgattgg ctgtttaagt tctacctttg ccacgattca
     6061 tttacctcct tttagatttt cgcataaaag acatgaggta tttgatttat taatcaaaga
     6121 aaccctcctg cctgtcgaca gaagggtaca agtcaaaatt caagataaat ctcgaaaatt
     6181 ccctcggtag gttctacgtt tacgccttgc ggcacctact gtcttcggca atatgcattg
     6241 caattatatc aaactatatt acggatgtca attactttcc gcaaaacttt tgcttggcgt
     6301 ttgataggtc ttgtaaattg ttttactgta actgtttaac tatcttactg tgtaatctta
     6361 accacgttta acttaacgcc aggtcccatt gtagatgtaa gagttgcgct cttaatgtac
     6421 tgacccttta agctagcagg ttttgcttta ttaattgcac ccataagaga ctggaagttg
     6481 tcgtttaact gctcgtctgt gaaagatgct tttccaactg gcacatggat aatgtttgtc
     6541 ttgtccaatc tgtactcaat cttaccggct ttgatatcgt taacagcttt tgttacatcc
     6601 atagtaactg ttcctgcctt tgggtttggc attaagccct ttggtccaag gacacgtcct
     6661 aaacgaccaa caacacccat catatcaggt gtagcaacta caacgtcgaa gtctaaccat
     6721 ccctcgttct ggatctttgg aattaactcc tcgcctccaa cgaagtcagc tcctgctgcc
     6781 tgtgcctcgt caaccttagt tcccttagcg aaaactaaaa ccttaactgt ttttcctgtt
     6841 ccgtgtggta atacaaccgc accacggatc tgctgctctg cgtgacgtcc gtcgcagcct
     6901 gttctaagat gaagctcgat tgtctcgtca aatttagctg tagcattttt ctttacgata
     6961 gagatagcgt cagcaacatc gtactgctct ttcttatcaa atgccttaac agcgtcttga
     7021 tacttctttc ctcttttcat gaaaaaataa cctcctggtg gtaatatcgg gagagggccc
     7081 tcccacattt acgtttcttt gttatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 tcaacaccca ttgatcttgc tgtaccagcg atcatgctca ttgcagactc aagtgaagct
     7261 gcgtttaagt caggcatctt aagctcagcg atttcctgaa cctgcgcctt tgtgatctta
     7321 gcaaccttct tcttgtttgg ctcgcctgaa ccagactgaa tcttgcaagc tttcttgatt
     7381 aataccggag caggtggagt cttagtaatg aaactgaaac ttctgtctgc atatacagta
     7441 ataactacag ggataattaa gtcgccctga tcggctgtct ttgcattaaa ctgctttgtg
     7501 aactctacga tgtttacacc gtgctgtcca agtgcaggtc caactggagg agctggagtt
     7561 gcctttccag cagggatctg caatttaatg tatccttcta ctttctttgc catttggaat
     7621 tgcctcctta atttgttttc ttaacatctg cgaaacttac ttctaccggt gtctcgcgac
     7681 cgaacagctc tacattaatt gtaactgtct gcttgtgata attcatagct gtgataacac
     7741 cgactgtatc cttccagaca ccgccgataa cgactatcat gtcaccctca gcaaaatcaa
     7801 ctaccacgtt ttccgtctta atacccaaag gtctcatctc ggcttctgtg agaggaaccg
     7861 gtttgcttcc cggacctaca aatccagtta cgcctctggt atttcgtaca acgtaccatg
     7921 tatcatcatt cataatcata tgaataagaa catagcctgg aaacatcttc ttggaaacct
     7981 gtttacgaac accgttcttc acctctgtaa cgtcctccaa tggaacacgc acttcaagga
     8041 tctggtcctc aagatgtctg ttttcgattg ttttgtcaat attagctttg actttgtttt
     8101 cataacctga ataggtatga actacatacc agttcgcttc tgtcataatc actttgcctc
     8161 cgttttaaaa atgtaaacca ataaggaaat ctacgccctg ctggataccc cagtcaagaa
     8221 gcgcaataat aagaccggta actactgaaa ccgccagtac tgcaannnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnntttgga gccttttcga ctttttcatt ctgtcccata atgtactcct
     8401 tcactcagtt cctagactat tttgtttcct tgtgcattgt atgagttctg cagaatctac
     8461 aatacttctt ggtttccatt ctgtccggat gagttttttt gtcctttgtc atgttgtaat
     8521 tacgacgctg acattctgtg cacgctaatg taatctttgt acgcacgact tccacctccg
     8581 tttaaattct ttgttgtttg tacccttaaa aaaaggcata aaaaaaagac ctatttccaa
     8641 gcctgtatac tatatcacag ttgagccgta accgtcaata gctattttaa aatttattgc
     8701 aaattctttt gaacagtgcc gctgtctgta cctttttgta tgtccggcag caattaattt
     8761 ttataaatct tacagataca taactaaata tagtacattt atctgaattt ttcaagtatt
     8821 ttgttgttgt aaatcctcaa ttatgacgat ataatatatg tgtataagta ggatttgata
     8881 attaaaaatt ttacatagct tattatagct gcagttaagc attagtaatc gcagctatac
     8941 aatataatat ttaaaggatt accatttaac ggattaaacg attttcacgg atgaaaggag
     9001 gggtctttat ggcaaagata gacagtgcct acgcttacta tgccagcact tatgccaata
     9061 aagaggtatc gcgctatgat tcgcacaaga aaagcgacct gcgcaagata tacaacagaa
     9121 taataaagac aaataaagag tcccctctgt acaagatttc caatatgtcc gaggccaaga
     9181 aatatgctat cgatataaag gaaagctcta aggccatctt aaatgccgtg gattcgcttt
     9241 ctgataaata cgcaggctcc tctgactcct tccagaagaa ggttgcggag tcatctaatg
     9301 aggatgctgt aggcgtgcgc tacgtgggtg acggctcaga gaataaagga gcagacggct
     9361 ttgatataga tgtcagacag ctcgccgcac cacagatcaa taccggcagc tacttagaca
     9421 gcaaggccct gtcctttata ccgggcagct attcctttga cataaatacc aatgcatcat
     9481 cctatgagtt ccagttcgga gtgagcaccg gtgaaacaaa cggcgaggtg caggacaagc
     9541 tcaagaggct cataaacgca tccggtcttg gcatcgaggc agacatagtc catgatgact
     9601 caggcaaggc agcgcttcag cttacatcaa tgcagacagg tctcagcgag actgaggatt
     9661 ctctctttag tgtggctccg tcagctaacg cagagtccat aagcatgatg aagcagctgg
     9721 gtattgataa ggtatcatcc cctgcccaca actctgactt ttcgctgaat ggcaccgcgc
     9781 actcatcctt gtccaacacc tttaccatca ataatacctt tgagctcacg ctcaaaaagc
     9841 cgaccacaga tacaggtgcc gcagccatcg gttttaaggc caattccgat gcagttgccg
     9901 acaatgtgca gactctggtt gacgcataca ataagatgat agacgtggca gatgattatt
     9961 cagtgaacga ctctgcagat gccacaaggc tgcttcgtga tataagctcc atcacaaagg
    10021 gctcacagtc aaagctttcc tacatcggtc tcatgacaga cgatgacggc aagctcacta
    10081 ttgaccgcga tatcctcgcc ggtgcgctta gtccggacag agctgatgac accttcaaca
    10141 ctcttacagc actaaaggat gcaataggtg acaaagcaaa atctgtgaca gtcaacccta
    10201 tgaactatgt gcagaaggtt gttgtggcat ataagaatcc cggacacaat ttcaacacac
    10261 catatatctc gtcaatttat tcaggcatga tgcttgacag ctacgcataa acggctgttg
    10321 taccaaatca gtacaacagc cgttatttta tattttttat aatagtggct gcagatctcc
    10381 caccattttt tcctcaagcg tcctgagtct ctctgcaatt gccacactct ttgaatcagc
    10441 atcgcgatat tcattgagac tgccgctgat tgtctctatg cccatattac agccctccgt
    10501 cagtaacttt gctgcctggg actgatcatc attcactgcc atcttcattg cagtctgcag
    10561 cttcgcagcc atctttgcaa acatacccgg ttccttaata tctgcacctg ctttgtcaag
    10621 catatccctg caatccactg acagcccggt gtgctcatca tagtacttgt ctatcagctc
    10681 acgaagctgc ttgtcatctg tgtgttctct tatatcgtcc atgctctcaa gtgccatctt
    10741 acagcccgct atgcaatcct gaagcaagag caccgaatca tgattctcaa tatttctgct
    10801 catagaaaaa cctccatacc ttttttatca gtatggaggt tttcatgaaa aaatattcac
    10861 tacacaatcg gaaactccag tttaacccaa aacttcccat gttccctttt aaagctcatg
    10921 cttccattca tgcgctgcat catcgcatct acgctcttaa gccctatccc ggcactttta
    10981 agaagtgttt tattgtcaca tacgccattg ccaatttcaa cgctaaatgt ttttttcgta
    11041 aaagctgttt tcataacaac aggcttctta ttgtccgcat atttatatat attatccgtc
    11101 agattggaaa atattcttgt caaaaatgcc atattaacag caacctttac ctgcttaaag
    11161 cttattcctt ctgcatcaac ctcataattg cttttcataa gctgagcaca cacatctgag
    11221 aggtaatctc caaatatata ctcaacatcc attgatgcat ccaattcaac aggtctttgt
    11281 gaaccggcaa ttgaaaaatc cagcagattg tctacctgag tcctgatatc caataccttt
    11341 tcacttataa aattaagatt tttcatattc tcggcatatg cttcgttctc cagtttcatt
    11401 acatcaacat acgtcataac gccagtcaat ggcgtgcgca ggtcatgagc catattacgc
    11461 atcagttcat ccttttcctg tcgcgctttt ttctcctgtt cctgtgatgc ttcaagctct
    11521 atttgatagc ctgctataat ttttctgatg cgtagataca caaatacaat tcccatcata
    11581 agagaaaatc ccacatcaaa aatcatcaaa gtgatataat attttatcgt aaatcctgta
    11641 tatataaata ctttcgcctc aatatcggag aactgaacag gcagctcata gcttcctgta
    11701 aatcgataat catacatatt tttagccgga gagataagct tatctacatt tgatttattg
    11761 tatattactt tcccctcttc ctgaatacta taaaacgaaa catcatgttc cgacatccat
    11821 tcatcatatt gctcgacatc tgtagtactt atatcattat cagacacata ctgttgaaac
    11881 tcatctatat atggcttttc attatagtat attaaatgag atgtaccttc aatataattg
    11941 ttcaacaaat caattcccag atagtataat agtccaaaaa caacgcatgt gcctataatc
    12001 gcaattgcaa atgtcaaaga cagctttttc tttaccctct ttaaatcatc tctcaaaacg
    12061 gtatcctctt ccccattctg tgtgtatgca tgcatcacca cacgctttta taagcttatt
    12121 ccttagattt ttaatatgta ctataatata cccataacag t
//
LOCUS       DPUF01000043            4445 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7360, whole
            genome shotgun sequence.
ACCESSION   DPUF01000043 DPUF01000000
VERSION     DPUF01000043.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7360"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="DHV81_01535"
     CDS             complement(<1..2003)
                     /locus_tag="DHV81_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCI93218.1"
                     /translation="MSEPYNHKAIEKKWQEYWKEHETFKTDVWDFSKPKYYALDMFPY
                     PSGVGLHAGHPEGYTATDIMSRMKRMQGYNVLHPMGYDSFGLPAEQYAVNTGHHPNGF
                     TQKNIETFSKQLRELGFDYDWSKMISTSDPEFYKWTQWIFKKLYEAGYAKHIDMPVNW
                     CEELGTVLSNDEVIDGKSERGGYPVVRKNMKQWVIDQPAFAEKLLEGLDEIDWPESTK
                     EMQRNWIGKSTGVEVKFQIVGGGEFSIFTTCIETIYGITFMVLAPDGDIVKGLMDRVE
                     NKEEVQAYIDETVKKSDMDRTELNKTKSGCELKGLHCINPVNGKEVRMFIGDFVLASY
                     GTGAVMAVPTHDQRDFEYAQAHDIEMIQVIDGADVSEHAFEKHDYLGKGCKLINSEEF
                     TGMVVEDAKEAITAKLEKMGVAKRTVNYKFREWIFARQRYWGEPVPCVYKEDGSIYFL
                     PDDELPLVLPELEDYKGKNGKAPLENATEWKQYDRNGVKGTRETSTMPGSAGSSWYYM
                     RYIDPHNDKEFANQELLKHWMPVDLYVGGPEHAVGHLMYSRIWNRFLFDQGLSPVKEP
                     FKKLVHQGMILGSNGIKMGKRFPEFVVNPSDVVEEYGADTLRLYEMFMGPLEVSKPWN
                     DSNVQGAKRFITRVWNFFTEPENIIEEDDGKLTRIYHQTVKKVT"
     gene            complement(2179..3244)
                     /locus_tag="DHV81_01540"
                     /pseudo
     CDS             complement(2179..3244)
                     /locus_tag="DHV81_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850900.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     assembly_gap    2308..2355
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2583..2666
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3302..3802)
                     /locus_tag="DHV81_01545"
     CDS             complement(3302..3802)
                     /locus_tag="DHV81_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCI93219.1"
                     /translation="MRRQALSLLVENNPGVTSRISGLFSRRGFNIDSFSSGVTADPRY
                     TRITIVASGDEQVLEQIEKQLAKLEDVLDIKKLEPGLSVCRELIMIKIRALDTQRQAV
                     MNVTEIFHGKIVDVTHDSMIIELIGTQDKLDKFIDLLSGYEILELARTGITGLTRGSD
                     DVVMLD"
     gene            complement(4047..>4445)
                     /locus_tag="DHV81_01550"
     CDS             complement(4047..>4445)
                     /locus_tag="DHV81_01550"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297510.1"
                     /note="PEP carboxykinase; PEP carboxylase; PEPCK;
                     catalyzes the phosphorylation and decarboxylation of
                     oxaloacetate to form phosphoenolpyruvate using ATP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCI93220.1"
                     /translation="FGQAFLELHPTKYAAELVKKMEKSGAKAYLVNTGWNGTGKRITI
                     KDTRGIIDAILSGDILNAPTKKIPMFDFEVPTELPGVDSGILDPRDTYADASEWEAKA
                     KDLAERFNKNFVKYTTNEAGKALVAAGPQL"
BASE COUNT         1104 a         1082 c          789 g         1338 t
ORIGIN      
        1 gtgaccttct ttactgtctg atggtagatt cttgtgagct ttccgtcatc ctcttcgata
       61 atattctcag gctctgtaaa gaaattccat acacgtgtga tgaatctctt tgctccctgt
      121 acgttgctgt cgttccaagg ctttgaaacc tcaagtggtc ccatgaacat ctcgtagaga
      181 cgaagtgtgt cagcgccgta ctcctcgact acatcactag gatttactac gaactccggg
      241 aatctcttgc ccatcttgat accgtttgat ccaaggatca ttccctgatg tacgagcttc
      301 ttaaaaggct cctttacagg ggataagccc tgatcaaaga ggaatctgtt ccagattctt
      361 gaatacatga ggtgtcctac cgcatgctcc ggtccaccaa catagagatc tacaggcatc
      421 cagtgcttta agagctcctg atttgcaaat tccttatcat tgtgtggatc gatatatctc
      481 atataatacc atgaggatcc tgccgagcca ggcatggtgg atgtctcacg agtacccttt
      541 acgccgtttc tgtcatactg cttccactct gtagcgttct caagaggtgc cttgccattc
      601 ttgcccttgt aatcctcaag ctcaggaagg acaagtggaa gctcatcatc aggaaggaaa
      661 tagattgaac catcctcctt gtatacacat ggaacaggct ctccccagta tctctgacgt
      721 gcaaaaatcc actcacggaa cttgtagttt acagttctct ttgccacgcc catcttctca
      781 agcttggctg tgatagcttc cttagcatcc tcaactacca ttccggtaaa ctcctctgag
      841 ttgatgagct tgcagccctt tccaagataa tcatgcttct caaatgcatg ctctgataca
      901 tctgcaccat caattacctg aatcatctca atatcatgag cctgtgcata ctcgaaatct
      961 cgctgatcat gagtaggaac tgccataaca gcacctgtac catagcttgc cagtacgaaa
     1021 tcaccgatga acattcgcac ttccttgccg tttacagggt tgatacaatg aagtccctta
     1081 agctcacagc ctgactttgt cttgttaagc tcagttctgt ccatatcgct cttctttaca
     1141 gtctcatcta tgtaagcctg aacctcttct ttgttctcga ctctgtccat gagacccttt
     1201 actatatctc cgtccggagc aagcaccata aatgtgatac catatattgt ctcgatacat
     1261 gtagtaaaga tagagaactc tccaccgcct acaatctgga atttgacctc gacacctgta
     1321 ctcttgccaa tccagtttct ctgcatttcc ttggtagact caggccagtc tatctcatca
     1381 agcccctcaa ggagcttttc tgcaaatgcc ggctggtcaa taacccactg tttcatgttc
     1441 ttgcggacta caggatagcc gccacgctct gacttgccat ctataacctc atcgtttgag
     1501 agaactgttc caagctcctc gcaccagttt acaggcatat caatatgctt tgcatatccg
     1561 gcctcataaa gcttcttgaa aatccactgt gtccacttgt aaaactcagg gtcacttgta
     1621 gaaatcatct ttgaccagtc ataatcaaaa ccaagctctc ttaactgctt tgaaaaggtc
     1681 tctatattct tctgagtgaa accgtttgga tgatgacctg tgtttacagc atactgctct
     1741 gccggtaaac cgaatgaatc atatcccatc ggatgaagta cattgtagcc ctgcattctc
     1801 ttcatacgcg acatgatatc tgtagctgta tagccctccg gatgtccagc gtgaaggcct
     1861 actcctgaag ggtaagggaa catatcaagt gcatagtatt taggctttga aaaatcccaa
     1921 acatctgtct tgaatgtctc gtgttctttc cagtattcct gccatttttt ctctatggcc
     1981 ttgtgattgt atggttctga catacttgcc tccttatatc aaaaacgctc cgcctctcaa
     2041 agagacgaag gcgtattttt aatccgtctg attaacattc ataattttac acatcacact
     2101 atattttgtc aagttttgct atataaaaaa cccggtgcat cactgccccg ggtcttattg
     2161 tcatatatat ctgacagatt agttgttgat gagcttgtcg ctctcatcat tccagctgta
     2221 aagcttacgg atttcctcac caaccttctc tgaaggatgc tcagaagcaa gctttctcat
     2281 agccttgaag tgtacctgac gtcctgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnngcaaa tgtaccatcc tgaatgtcag aaagaatctt cttcatagct
     2401 ttctttgtct cagctgtaac aatcttaggt cctgttacat aatcaccata ctcagctgta
     2461 ttagagatag agtatctcat acctgcgaag cctgactggt agataaggtc tacgatgagc
     2521 ttcatctcat ggatacactc aaagtatgcg tttcttgggt cataaccagc ctcgcaaagt
     2581 gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnntctg tctctgtctc tgtacggaat gttgtctcaa
     2701 gaagtccggc tcttgctccg ccgattccaa gaccatatgc aagtgcaagg tcccatgcct
     2761 ttcctgtagc atcctgctct acagcgataa gacaaggtgt tccttttcct gcctggtact
     2821 ctgaacgtac tgtatgtccc ggagccttag gtgcgatcat agctacatct acatcctttg
     2881 gtggcttaat gcatccaaag tggatgttga agccgtgagc gaacataagc atgttgcccg
     2941 gctcaaggtt tggctcgatg tccttcttgt acatatctgc ctgtaactca tcatttataa
     3001 gaatcatgat gatatcagcc tgctttgcag cctctgcagc gacatatacc ttgaagccct
     3061 gctcttctgc tctcttccat gacttgctgc cctcataaag accgatgatg acattgcatc
     3121 ctgaatcctt taagttaagt gcatgtgcat gtccctggct gccgtaaccg ataatggcaa
     3181 ttgtcttgcc ctctaataat gaaaggttac aatcatcctg ataaaataat cttggttctg
     3241 ccattttgtt ttcctcctgt taatttaagt aaactattta tatgtaagga ttgtcctgtg
     3301 actaatccaa cataactaca tcatcggaac ctctggtaag acctgtgatt cccgttcttg
     3361 cgagctctaa tatctcatag ccgctaagta agtctatgaa cttatcaagc ttgtcctgtg
     3421 tacctatgag ctcaataatc atagagtcat gagtgacatc aactatcttg ccgtggaaga
     3481 tctcagtcac attcataact gcctgtcttt gtgtatcgag tgctcttatc tttatcataa
     3541 taagctccct gcacactgag agtcccggct caagcttctt gatatcaagt acatcctcaa
     3601 gcttcgccaa ctgcttctct atctgctcaa gcacctgctc atcaccgctt gcaacaattg
     3661 tgatacgtgt gtagcgcgga tctgctgtaa cccctgaaga gaaactatca atattaaatc
     3721 ccctgcgact gaacagtcct gaaatacgac tggtcactcc gggattgttc tcaactaaaa
     3781 gagataatgc ctgtcttctc atcgaaaaac cttcctcaca atttctatta tttctgttca
     3841 catgatgata atattactat aatctgtatt tgtcaagcaa tatcgttaat ataacttaaa
     3901 ttcatattat caataacaat atttatacat ccataacctc gataaatgcc tgttttataa
     3961 cgttttatac agtatcttca aaaaaagccg ggcgacatac aaccgcccgg ctttagaatt
     4021 atctaatata attctgctgt aatatattat aactgtggtc ctgctgcaac aagtgcttta
     4081 ccagcctcat ttgttgtgta cttaacaaag ttcttgttga atctctctgc aagatcttta
     4141 gcctttgcct cccactcaga agcatcagca tatgtatcac gaggatcaag gattcctgaa
     4201 tcaactcccg gaagctctgt aggaacctcg aaatcgaaca tagggatctt ctttgttgga
     4261 gcattaagga tatcaccaga aaggatagcg tcgatgattc ctcttgtatc cttgattgtg
     4321 attctctttc ctgttccgtt ccatcctgta tttacaaggt atgccttagc gcctgacttc
     4381 tccatcttct taacgagctc tgcagcgtat tttgtaggat gaagctcaag gaatgcctgt
     4441 ccgaa
//
LOCUS       DPUF01000044            6057 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8778, whole
            genome shotgun sequence.
ACCESSION   DPUF01000044 DPUF01000000
VERSION     DPUF01000044.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8778"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /gene="dnaK"
                     /locus_tag="DHV81_01555"
     CDS             complement(<1..203)
                     /gene="dnaK"
                     /locus_tag="DHV81_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602474.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCI93221.1"
                     /translation="MGKVIGIDLGTTNSCVAVLENNQPVVITNSEGQRTTPSVVAFTK
                     NGERLVGEAAKRQAAVNVDRTFFS"
     assembly_gap    310..354
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(478..2406)
                     /locus_tag="DHV81_01560"
     CDS             complement(478..2406)
                     /locus_tag="DHV81_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCI93222.1"
                     /translation="MTIRDLLAAESINLNGTPAGKTEALNQCIDLMAKSGKIADVEKY
                     RKGVFAREEEGTTGIGMGIAIPHCKSDAVTKAGLAAMVVKDGVDFESLDGTPAKIIFL
                     IAAPNTEDNVHLQVLSKLSVMLMDEQFTNSLINAGSVDEFLNIIDSAEKAKDEKEAAK
                     EAKAKEPVEVKKDDVFIVAVTACPTGIAHTYMAAEAIEKKAKELGYQVKVETRGSGGA
                     KNVLTDDEIAKAAGVIVACDTNVPTDRFDGKKVIECQVSDGINKAEELIKRIASGDAP
                     VFKASGKKEASHSSVGGKESVGHQIYKHLMNGVSHMLPFVVGGGILIAIAFLIDGFSV
                     DLNSLPADQRANFGTITQGAALFKGIGGTAFGFMLPILAGFIAMSIADRPGLAVGFVG
                     GSIAANGTSGFLGALVAGFVAGYIVNLLKKIFSKLPESLDGVKPVLLYPLLGIFLIGV
                     IMQFVVEPPIGALNTAINNGLNGLNGASAVVLGVLLGGMMAIDMGGPVNKAAYVFGTA
                     SIAAGNYNIMAAVMIGGMVPPLAIALATIFFKNKFTAEERKAGPTNFIMGLSFITEGA
                     IPFAASDPLHVLPACAVGSAVAGGLSMAFGCTLMAPHGGIFVVPTIGNPLMYLVALVI
                     GAFIACGLLGLLKKKVSE"
     gene            complement(2472..3389)
                     /gene="pfkB"
                     /locus_tag="DHV81_01565"
     CDS             complement(2472..3389)
                     /gene="pfkB"
                     /locus_tag="DHV81_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCI93223.1"
                     /translation="MIYTVTFNPSLDYIVDVTDFKTGVVNRTTGEKIFAGGKGINVSM
                     VLKNLGMSNTSLGFTAGFTGNEIKRLLAKKGVDTDFIEVEDGISRINVKLRSNEESEI
                     NGQGPVITEDNIKKLYEKLDCLKESDVLVLAGSIPDVMPSSMYMDIMKHLEGRGINIV
                     VDATRNLLTNVLPYKPFLIKPNNHELGEIFGVEITDKDDVIKYAKKLQEQGARNVLVS
                     MAGDGAVLVTEDGQEFKANAPKGKLKNSVGAGDSMVAGFVYGYLKSNDYKQAFIYGVC
                     TGSASAFSEELATKPEVEALIDKWESASN"
     gene            complement(3391..4134)
                     /locus_tag="DHV81_01570"
     CDS             complement(3391..4134)
                     /locus_tag="DHV81_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCI93224.1"
                     /translation="MLAEDRCKKILDILDSMGSVTVQQLMDELDTSESTIRRDLVAMD
                     KKGYLTKVHGGAIANNTNIHTQDEKVINRLKQNRSEKEQIARYAASLIVDNDFVYIDA
                     GTTTAVMIDYITAKNVVFVTNSLTNAKRISDRGYTVYILGGEFKSTTEAIVGDEAVVT
                     LDKYNFTKGFWGTNGITVKNGFTTPEIKEAMVKKKSMENSKEKYILADDSKFSQVSSI
                     KFADFEDAVIVTNALSNDNYKKYKNIKEV"
     gene            4716..5705
                     /locus_tag="DHV81_01575"
     CDS             4716..5705
                     /locus_tag="DHV81_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCI93225.1"
                     /translation="MKQLNWATLGCGVIANELAHALSKEGRTLYSVANRTHEKAVAFA
                     QKYGISKVYDNIDDVFYDPDVDIIYISTPHNTHINYLRKALSNGKHVLCEKSITLNIA
                     ELDEAVSLAKENNVILAEAMTIYHMPVYKKLNEIIASGQLGRLNLIQVNFGSYKDYNM
                     TNRFFSRKLAGGALLDIGVYSLSLIRWFFKETPTQVSSQVKYAPTGVDEQAGILLMND
                     AVEMATVTLSLHSKQPKRATLSFDKAYVEIFEYPRGMQATITYTEDGHKEVIDAGDTS
                     DALYYEVRDMEKAVSSASSDEIETLMHCQYTYDVMKIMSDIRAEWGMKYPEEE"
     gene            complement(5953..6023)
                     /locus_tag="DHV81_01580"
     tRNA            complement(5953..6023)
                     /locus_tag="DHV81_01580"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(5989..5991),aa:Cys,seq:gca)
BASE COUNT         1801 a         1339 c          995 g         1877 t
ORIGIN      
        1 gagaaaaatg tcctgtccac attgactgct gcctgcctct ttgcagcctc gccaaccagt
       61 cgctctccat tctttgtaaa agccactaca gacggagttg ttctctgacc ctcgctgtta
      121 gttatgacta ccggctgatt gttctccaat actgccacac agctgtttgt tgttcctaaa
      181 tctataccta taaccttacc catgattttc ctcactgttt tttatattta agtctcataa
      241 ataattgctt tttatgactt actatttcat cctaatacta ctttaagctt cgaaaattca
      301 ctcgtcaagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgttat
      361 ttcagagaat gtttatattt ttaatttttg tataacaaca taacaacaaa gacaccgccg
      421 aagcagtgcc tttgttgatt agtttataga ttggattaaa tgataattga ggattgctta
      481 ttcacttact tttttcttga gtaatccgag taatccacat gcaatgaagg ctccgattac
      541 taatgctaca agatacatta acggatttcc gattgtaggt actacgaaga ttcctccgtg
      601 aggtgccatg agtgtacatc caaatgccat tgaaagtcca cctgccactg ctgaaccaac
      661 tgcacatgcc ggaaggacat gaagcggatc agatgctgcg aatgggattg caccctctgt
      721 gataaatgat aatcccatga taaagttcgt aggtcctgcc tttctctcct ctgctgtgaa
      781 cttgttcttg aagaagattg ttgcaagagc gattgcgagt ggtggaacca taccaccaat
      841 cataactgct gccatgatat tgtagtttcc ggcagcgatt gaagctgtac caaatacata
      901 agcggctttg tttacagggc cacccatatc tattgccatc attcctccga gaagtactcc
      961 gagaactact gcgcttgcac cgttaagtcc attaagaccg ttgttgatag ctgtatttaa
     1021 agctccgatt ggaggctcaa ctacaaactg cattataacg cctatgagga atataccaag
     1081 cagcggataa agaagaaccg gcttcacacc atcaagactc tcaggaagct tagagaatat
     1141 cttctttaag agatttacaa tatatcctgc aacgaaacct gcaaccagtg ctccaaggaa
     1201 gcctgatgta ccgtttgctg cgattgaacc gcctacgaaa ccaactgcaa gacccggacg
     1261 gtcagcaata ctcatagcga taaagcctgc aaggatagga agcataaatc caaatgctgt
     1321 tccaccgatt cccttaaaca atgctgcgcc ctgtgtaata gtaccgaaat ttgctctctg
     1381 gtcagccggc agtgaattaa ggtcaacact gaatccatcg ataaggaatg cgattgcgat
     1441 aagaatacca ccaccaacta cgaatggaag catatgtgaa acaccattca tcagatgctt
     1501 gtaaatctga tgtccaacgc tctcctttcc accaactgat gaatgtgaag cctccttttt
     1561 acctgatgcc ttaaatacag gagcatcacc ggaagcaatt ctctttatga gctcttctgc
     1621 cttattgatt ccgtcagaaa cctgacactc aataaccttc tttccgtcaa agcggtctgt
     1681 aggtacattt gtatcacagg caactataac acctgcagcc tttgcgattt catcatctgt
     1741 gagcacattt ttggctccgc ctgagcctct tgtctcaacc tttacctgat atccaagctc
     1801 cttggctttt ttctcgatag cctcagcagc catataagta tgggcaattc ctgtaggaca
     1861 ggctgtaact gcaacgataa atacatcgtc cttcttcacc tcaacaggct ctttcgcctt
     1921 tgcctctttg gcagcctctt tctcatcctt tgctttttca gcactgtcga tgatatttaa
     1981 aaactcatca actgaaccgg cattgataag agaatttgta aactgctcgt ccatcagcat
     2041 aacagagagc ttgctaagca cctgaagatg cacattatcc tctgtgtttg gtgcagcgat
     2101 aaggaaaata atctttgccg gtgtaccatc gagtgattca aagtcaaccc catcctttac
     2161 aaccattgcc gcaagacctg ctttagtaac agcatcagac ttacaatgag gaattgctat
     2221 acccattcca attcctgttg tgccttcctc ctctcttgca aaaacaccct tgcgatactt
     2281 ctccacatcg gctattttgc cactttttgc catcaaatca atacactgat tcaatgcttc
     2341 ggtttttccg gcaggcgtac cattcaaatt gatactctca gctgccaata aatccctgat
     2401 tgtcatacat cattcctcct aatagaatgt attaatttaa ttatttatat caaccttttt
     2461 gataccgttg tttaattgga agcactctcc catttatcaa taagcgcttc aacctcaggc
     2521 tttgtagcaa gctcctcaga aaatgcactt gcacttcctg tacatactcc ataaataaat
     2581 gcctgcttat aatcatttga tttgagatat ccgtaaacaa atccggctac catcgaatca
     2641 cctgctccta cagagttttt gagctttcct ttaggtgcat ttgccttaaa ctcctgtcca
     2701 tcctcggtca caagcactgc tccgtcgcct gccattgaaa caagcacgtt tcttgcgccc
     2761 tgctcctgaa gcttctttgc atactttatc acatcgtcct tgtctgtaat ctcaaccccg
     2821 aatatctcac cgagctcatg attgtttggc ttaatgagga acggcttata tggaagcacg
     2881 tttgtgagca gatttcttgt cgcatcaacc acgatattta ttccgcggcc ctcaagatgc
     2941 ttcatgatat ccatatacat tgatgaaggc atcacatcag gaatacttcc tgcgagcaca
     3001 agcacatcag actcctttaa gcagtcaagc ttctcataaa gctttttaat gttatcctca
     3061 gtgattaccg gtccctgacc attaatctcg gattcctcat ttgagcgaag cttcacattg
     3121 atacgtgaaa taccatcctc cacctctata aaatcagtat caacaccttt ttttgctaga
     3181 agtcttttaa tttcatttcc tgtaaaaccc gctgtaaaac caagtgaagt attgctcatt
     3241 ccaagattct taagaaccat ggaaacattg atacccttac cacctgcaaa aatcttctct
     3301 ccagtcgttc tattgactac tcctgtctta aaatctgtta catcaacaat ataatctaat
     3361 gatggattaa aggtaactgt gtaaatcatg ttagacctcc ttaatatttt tgtatttctt
     3421 atagttgtca ttgcttagtg catttgtaac aattacagca tcctcaaaat cagcaaactt
     3481 gatacttgat acctgtgaaa acttactgtc atccgcaaga atgtatttct ccttgctatt
     3541 ctccatggat ttctttttaa ccatggcctc ctttatctcc ggagttgtaa atccattctt
     3601 cacagtaatt ccgttggttc cccagaagcc cttcgtaaaa ttgtatttat cgagcgtcac
     3661 aacggcctca tctccgacaa tagcctcagt agtagacttg aactcgccac ccagaatata
     3721 tactgtatat cccctatcgg aaattctctt ggcattagtc agagagtttg taacaaaaac
     3781 cacattcttt gcagtaatat aatcaatcat gactgctgtt gtagtaccgg catctatata
     3841 aacaaaatca ttatccacaa taagtgatgc cgcatatctg gcaatctgtt ccttttcgct
     3901 gcgattctgc ttaagcctgt tgataacctt ctcatcctga gtatggatat tcgtattatt
     3961 ggcaatcgca ccaccatgta ccttggtgag ataccccttc ttgtccattg caaccagatc
     4021 acgtctgatt gttgactcgg atgtatcaag ctcatccata agctgctgaa cagtcacact
     4081 tcccatcgaa tctaatatat cgagtatttt tttgcatcta tcttccgcca gcatctaatc
     4141 accccagttt atcaaattat tcagagactt attttataag ccatttattt aatgcttatt
     4201 ttcaaattac tgtattaaac atttcaacat tcatttaact tgtagtataa ttatattttc
     4261 ataatccgtc attgtcaatc ataatccgtc atttatattc acaaataatc acactaaaaa
     4321 ttgttgaaaa tatataaagt aagttttaaa attgtgcact atatgcaaat acaacattga
     4381 ttttagtgca gaatataaca tatatatcat taatacttgc ctaattacta attttagtat
     4441 cttttctatt actaatttat aatctaaaat atagttcaaa ttaactaatt ttaatttagt
     4501 cactatcaaa tttgactgtt tttgactgtt ttgaatctaa atgcattgta attcataatt
     4561 catcatgcta gaatatgttc attatggtac atattttatg attaaagggt caaaaggtat
     4621 catcaatgag tttatatttt gttatttcac accattttaa ataatttatg taccatcaca
     4681 gctacaatac tttaagttaa aaaggaggat ttcacatgaa acagcttaat tgggcaacac
     4741 tcggctgcgg tgtaatagct aatgagctcg cgcatgctct ctccaaggaa ggacgcactc
     4801 tttattcagt tgccaacaga actcatgaaa aggccgtagc ttttgcacaa aaatatggca
     4861 tttcaaaggt atatgacaac attgatgacg tcttttatga tcctgatgtg gatatcattt
     4921 acatctccac tccacacaat actcacatca attatctaag aaaagccctt tctaatggaa
     4981 agcatgtact ctgtgagaaa tctattactt taaatatcgc agagctcgat gaagccgtct
     5041 cactggccaa agaaaataac gttattcttg ctgaagccat gactatatat catatgcctg
     5101 tttataaaaa gctaaacgaa ataattgcaa gtggacaact cggcaggcta aatttaattc
     5161 aggtcaattt tggaagctac aaggattaca atatgacaaa ccgatttttc agtcgtaagc
     5221 ttgctggagg agcattgctt gatattggag tttactcact ttctcttatc cgctggttct
     5281 tcaaggaaac accaacacaa gtatcgtcac aggttaaata tgcaccaacc ggtgttgatg
     5341 aacaggcagg tattctgctt atgaatgatg cagtggaaat ggcaactgtc acattatctc
     5401 tacattccaa gcagccaaaa cgcgctacgc tctcttttga caaggcatat gtggaaatct
     5461 ttgagtatcc aagaggaatg caggcaacta ttacatatac agaggatggt cataaagaag
     5521 taattgatgc cggtgataca tcagatgcac tgtattacga agtacgtgat atggaaaaag
     5581 ctgtaagctc agcatcatca gatgaaatag aaaccctcat gcactgtcaa tatacatatg
     5641 atgtaatgaa aattatgtca gatataagag ctgaatgggg tatgaaatat cctgaagagg
     5701 aataataata ctactacaac tattcaaaaa aggataagag ttgttaaaaa agagaaatat
     5761 aattctgttt atcaggctta tatttctctt tattaattta gtgtaacatc ctgaaaaata
     5821 atttagaaaa ttaaaaaata atgaaatgtg tacaacatat attacgaata ttacataaat
     5881 tcacaatata ttttatttgc aaattatata aaagaaagac cacatcataa actgatgtag
     5941 tccttttgag agaggtgaca gccggatttg aaccggcgat gacggtgttg cagaccgggg
     6001 ccttaccact tggctatgtc accatatatt atactaaaaa actccccgag tagggct
//
LOCUS       DPUF01000045            6246 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12274, whole
            genome shotgun sequence.
ACCESSION   DPUF01000045 DPUF01000000
VERSION     DPUF01000045.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12274"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="DHV81_01585"
     CDS             complement(<1..1781)
                     /locus_tag="DHV81_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCI93226.1"
                     /translation="MENNKIKDKIDELVESLNRASKAYYNGADEIMPNYEWDALFDEL
                     TQLEKETGYIRHDSPTQNAGYEAEAGNREPHEYPALSLAKTKSIEELKKWAGDMPIWL
                     SWKLDGLTLVLTYDGGRLVKILTRGNGTVGSNITFLQDAISGFPKEIPYKGHMVVRGE
                     ATISYTDFKLLNDTIEDDDEKYANPRNLASGTLNLDDVEEVKRRHVVFYAFTLVHIDD
                     DIISWGDRMSYLSDMKFNVVKREATDAAGLEDAVKRWTRDVESGRMDVPVDGLVICYD
                     DTAYAAGGSVTGHHATRAGFAFKWQDEAVDTRLRYIEWSCAVSTISPVAVFEPVQIEG
                     TTVSRASLCNLTEIERLGVGKECTLSVIKANKIIPKCIAVKDAVGAVEIPKECPVCHH
                     PTRIFVSKNSGVKTLHCTNPDCTAKNVKKFSRFVSKSGMDIDGLSVQTMLKFINEGFI
                     KQFPDIYHLPEHFDKISSMEGFGEKSCMNMQAAIEKSRHVHPVNLIFALCIPLIGTDA
                     GKKIVNAIGFDGFADRMRNATDFVDIDGIGQEKSGSILEWYANPKNSAMFEALIKELD
                     IEKVDIKDMSEGSLNGKTFVITGDVHD"
     gene            complement(1805..2623)
                     /locus_tag="DHV81_01590"
     CDS             complement(1805..2623)
                     /locus_tag="DHV81_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCI93227.1"
                     /translation="MEENGVRINKFLSEAGVCSRREADRQIEAGKVGIDGVTAKTGDR
                     VLKGQTVTYNGQVVSKEEEMILIALNKPAGIVCTAEKREKNNVIDYLNYPKRIYPIGR
                     LDKDSEGLLLLTNNGEIVNKMMRSGNRHEKEYIVTVNKTISDSFIRGLAGGVPLVELG
                     VTTRKCHVKKLSSKQFKIILTQGYNRQIRRMCEYFGYRVVKLERVRIMNIELGDLPRG
                     KYRAVTPLEYKGLKRLIADSSNQPVMPDTNKNHSDKKYTDKKHTDKKYPDRRAK"
     gene            complement(2789..>3050)
                     /locus_tag="DHV81_01595"
     CDS             complement(2789..>3050)
                     /locus_tag="DHV81_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93228.1"
                     /translation="QTTDTNAANQQTAEQPQAPAEYQVVTKDVVKVRDGASTDAKELG
                     LLKEQVTLTAYGTEGDWTIVSYSAGTNGRAYIKTENLQTVGQ"
     assembly_gap    3051..3239
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3240..3979)
                     /locus_tag="DHV81_01600"
     CDS             complement(<3240..3979)
                     /locus_tag="DHV81_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93229.1"
                     /translation="MKDFFNKETLDNIKQFCAENKKYVGVAGVLVGLILILIVVVAGG
                     NNGRQSNTEAGSQTEISVKDFEFEKDYTNDAKDDISTLLADYYKAYVGDDLDTLGKLA
                     TPMSDNEKSYIGVVSQYYESVNDITPYTKNGLKKGTYFVSVKNSIKFTGVDTQAPTLD
                     FFYIETGKDGKLFINNVYSNFNRTYCENELDDDIMELISKYTASSEFADLHEEVQKAY
                     EQAVGSDANLKTMLETTLTGAIKQWYTSS"
     gene            complement(4019..4091)
                     /locus_tag="DHV81_01605"
     tRNA            complement(4019..4091)
                     /locus_tag="DHV81_01605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(4056..4058),aa:Arg,seq:ccg)
     gene            4326..5357
                     /locus_tag="DHV81_01610"
     CDS             4326..5357
                     /locus_tag="DHV81_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCI93230.1"
                     /translation="MSFTFLNQLPTPAQIKEEYPLSKELTELKAKRDAMISDVICGKD
                     DRFLVIIGPCSADNEDSVCDYVSRLTKIQEDVKDRVIIIPRVYTNKPRTTGEGYKGIA
                     SQPDPEKAPDMVEGLIAMRKMHIRAIAESGLTCADEMLYPENWGYVEDLLSYVAIGAR
                     SVEDQQHRLTVSGFDVASGMKNPTSGDFSVMLNSVYAAQHPHHFVYRGYEVETSGNPL
                     THVVLRGAVSKHGNTTTNYHYEDLIRLHEMYDKMDVVNPAAIIDTNHSNSGKQFKEQI
                     RIAKEVMHNRQLSSDIKSLVKGLMIESYIEEGSQKIGEHVYGKSITDPCLGWEDSKKL
                     IYDIAEMNS"
     gene            complement(5437..5976)
                     /locus_tag="DHV81_01615"
     CDS             complement(5437..5976)
                     /locus_tag="DHV81_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCI93231.1"
                     /translation="MIDEMKRQKRELVYKGSILDIYKDTMQFANGKTEEWDFVSHRKG
                     AAAVLPVLDDGRIIMVRQYRNALERETIEIPAGCRDFVGEDTRLCAERELKEETGYSS
                     DDISFLLSLRTTVAFCDEEVDVYLAKNLKKGEQHLDDAESIDVEIYTLKELCDMIYAG
                     KIQDSKTVSAVLAYSNMIK"
     gene            complement(6025..>6246)
                     /locus_tag="DHV81_01620"
     CDS             complement(6025..>6246)
                     /locus_tag="DHV81_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014120145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butanol dehydrogenase"
                     /protein_id="HCI93232.1"
                     /translation="DDLNIPHCIKNYGADSYPTEQGFVPEEVFLERLPEIAANAILDA
                     CTGSNPRQPSQEEMEKLLKCCYYDTEVDF"
BASE COUNT         1509 a         1483 c         1114 g         1951 t
ORIGIN      
        1 aaatcatgca catcgcctgt gatgacaaag gtcttgccat ttaagctgcc ctccgacata
       61 tcctttatat cgaccttttc tatgtcaagc tccttaatca gtgcttcaaa catcgcactg
      121 tttttgggat ttgcatacca ttcaagtatg gagcctgact tttcctggcc tataccgtct
      181 atatccacaa aatctgtggc atttctcatt ctgtccgcga agccatcaaa acctattgcg
      241 ttgacaatct tcttgcctgc gtcggtacct ataagaggta tacagagtgc aaatatcaga
      301 ttgaccgggt ggacatgtct gcttttttcg atggcagcct gcatattcat gcatgatttt
      361 tctccaaagc cttccatgct gcttatctta tcaaaatgct ctggcagatg gtatatgtca
      421 gggaactgtt ttataaatcc ctcatttata aattttagca ttgtctgtac cgaaagtcca
      481 tctatatcca ttccgctttt gctgacaaat cttgaaaact ttttgacatt cttggctgta
      541 cagtcagggt ttgtgcaatg cagcgtcttc acaccgctgt ttttactcac aaatatgcgt
      601 gtcggatgat gacacaccgg acattccttc ggtatctcaa ctgctccaac cgcatccttt
      661 acagctatgc atttcggaat aatcttgttg gcttttatca cggaaagtgt acactctttt
      721 cccactccaa gtctttctat ctcagtcaga ttgcaaagcg aagctctcga aaccgtcgta
      781 ccctctatct gcaccggctc aaaaacagcc accggcgata ttgtcgaaac cgcacacgac
      841 cattcaatat aacgaagcct cgtatctaca gcctcatcct gccacttgaa ggcaaaacct
      901 gctctcgttg catgatgtcc cgtgacactt ccgcctgcag cgtaggcagt gtcatcatag
      961 catatgacaa gtccgtccac aggcacatcc atcctgccac tctcgacatc ccttgtccag
     1021 cgttttacag catcctcgag gcctgccgca tctgttgcct cccttttgac aacattaaat
     1081 ttcatatcac taagatagct cattctatcc ccccatgata tgatatcatc atctatatgc
     1141 acgagtgtaa acgcataaaa cactacatgt ctgcgcttca cctcttccac atcatcgaga
     1201 tttaatgtac cggatgcaag gttccttgga ttggcatatt tctcatcatc gtcctcgatt
     1261 gtatcattta acagcttaaa atccgtgtag gaaattgtcg cttcccctct tacaaccata
     1321 tgtcccttgt atggtatctc ctttgggaag ccgcttatgg catcctgcaa aaaggtgatg
     1381 ttacttccta cagttccatt tcctctcgtc agtattttca caagcctgcc accgtcatat
     1441 gtgagcacga gtgtcagtcc gtcaagcttc catgaaagcc agattggcat atcacctgcc
     1501 catttcttaa gctcctcaat gctctttgtc ttggcaagtg aaagtgccgg atactcatgc
     1561 ggctcacgat ttccagcctc tgcctcatag cctgcattct gtgtcggact gtcatggcgg
     1621 atatatcctg tctctttttc aagctgtgta agctcatcaa acagtgcatc ccactcgtag
     1681 tttggcatta tttcatctgc accgttgtag taggcttttg acgcgcggtt aagactttca
     1741 acaagctcat ctatcttatc ttttattttg ttattctcca tgctgtaata atcccctgtc
     1801 tgctttactt cgctcttcta tccggatatt tcttatccgt atgtttctta tccgtatatt
     1861 tcttatcaga atgattctta ttcgtatcag gcataaccgg ctgattcgag gaatccgcaa
     1921 taagtctttt gagtcccttg tattcaagtg gtgtcactgc cctgtacttt cctcttggca
     1981 ggtcaccaag ctcaatattc atgattctca ctctctcaag cttaaccaca cggtagccaa
     2041 agtattcaca catccgtctt atctgtctgt tgtagccctg tgtcagaata atcttaaact
     2101 gctttgagga cagctttttc acatggcact ttctggttgt cacaccaagc tctacaagcg
     2161 gtacgcctcc tgcgagccct ctgatgaagc tgtcagatat tgttttgttt actgtgacaa
     2221 tatactcttt ttcgtgtctg ttgccggagc gcatcatctt atttactatc tcgccattgt
     2281 tggtcagaag taaaagtcct tctgaatcct tatccagcct gccaatggga taaatgcgct
     2341 ttgggtaatt aagataatct atcacgttgt ttttttccct tttttctgct gtgcagacta
     2401 tgccggccgg cttattgagg gcaatcagta tcatctcttc ttcctttgat actacctgtc
     2461 cattatatgt gacagtctgt ccctttagaa ctctgtcacc tgtctttgct gtcacaccgt
     2521 ctattcctac tttgccggct tctatctgcc tgtctgcctc acgccttgag cacacgcccg
     2581 cttcactgag gaatttattt attcttacac cattttcttc cataccatat cctcttttta
     2641 gattgcttta tccgatatac ataaaccaaa cagcagcctt ttctactctt tacagcagcc
     2701 atttgctatg tagattaaaa cacggagacc tttaagcctc cgtgttgctt cttatgattg
     2761 tatgcacatc tgttacttta tattgctttt actgtcctac tgtctggaga ttctctgtct
     2821 tgatataagc tctgccattt gttccggcgc tgtaggatac tatagtccag tctccctctg
     2881 tgccatatgc tgtaagtgtt acctgttcct ttaagagacc aagctccttt gcatctgtgc
     2941 ttgcaccgtc acgtaccttt acaacatcct ttgtcactac ctgatactca gccggtgcct
     3001 gtggctgctc ggctgtctgc tgattggctg catttgtatc tgtggtctgt nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3241 acgatgtgta ccactgcttt atagcacctg tgagtgttgt ctcaagcata gtctttaaat
     3301 tggcatcaga accgaccgcc tgctcatatg ccttttgtac ctcctcatga aggtctgcaa
     3361 actcagatga agcagtatac ttagaaataa gctccataat atcgtcatcg agttcattct
     3421 cgcagtatgt tctgttgaaa ttactgtata cattgtttat aaagagcttt ccgtccttac
     3481 ctgtctctat ataaaagaag tcaagtgtag gtgcctgtgt atcaactccc gtaaacttta
     3541 tgctgttttt aacagacaca aagtatgttc cctttttcaa accattcttg gtgtaaggtg
     3601 taatgtcatt gactgactca tagtactggc tgacaacacc aatatagctc ttctcgttgt
     3661 cgctcatagg tgtcgcaagc ttgccaagtg tatcaaggtc atcacccacg tatgccttgt
     3721 aataatcagc aagtaatgtg ctgatatcgt ctttggcgtc atttgtgtaa tccttttcaa
     3781 actcaaaatc cttcaccgaa atctctgtct gtgatccggc ttcagtattg gattgtctgc
     3841 cattattgcc tcctgcgaca actactataa gtataagtat gagtcctaca agtacacctg
     3901 ctactccaac gtatttttta ttttctgcgc aaaattgctt tatattatcc aatgtttcct
     3961 tgttaaaaaa gtccttcata tggttcctcc attaacccaa catatcaaaa tcaaagtatg
     4021 tgaccgacgc gaatcgaacg cgcactcgcc ggagtcggag tccggaattt tatccattaa
     4081 actacggtca caaaaaaagt taccaactat ttatatcaca tcttttagtt tttttcaatg
     4141 ttactttttt cttattataa ctttttatgc atactattat aattgataaa aatttgacaa
     4201 catatttcat ttatcttatt atttaactat tcggacatta ttatatattg gaaatttttt
     4261 ccgatatgcc attataatat accattttaa ttaataaaat cactatttat ataaggagca
     4321 gtattatgag tttcacattt ttaaaccagc ttccaactcc tgcacagatc aaggaagaat
     4381 accctctgtc aaaggagctt accgagctca aagccaagcg tgatgcaatg atttcagatg
     4441 tcatttgcgg aaaggatgac agatttcttg tcattatcgg accatgttca gccgataacg
     4501 aggattcagt ctgtgactat gtcagccgcc tgacaaagat tcaggaggat gtaaaggacc
     4561 gcgtaattat catcccacgt gtatatacaa ataaaccaag aacaaccggc gagggctaca
     4621 agggtatcgc ctcacagcct gaccctgaga aggctcctga tatggttgag ggtcttattg
     4681 ccatgagaaa aatgcacatc cgcgccatcg cagaaagcgg gctcacctgt gcagatgaga
     4741 tgctttatcc tgagaactgg ggctatgttg aggatctcct ctcatatgtc gcaattggtg
     4801 ctcgttctgt tgaggatcag cagcatcgtc tcaccgtcag cggcttcgat gtggcaagcg
     4861 gtatgaaaaa ccctacaagt ggtgattttt ctgtcatgct caattctgtt tatgcagcac
     4921 agcacccaca tcattttgtt tacagaggct atgaggttga gacatcagga aatcctctga
     4981 cacacgttgt tttaagaggc gcagtcagca agcatggcaa cactacaaca aactaccatt
     5041 acgaggattt aatccgtctg cacgaaatgt acgacaagat ggatgttgtc aatcctgctg
     5101 ctatcataga tacaaaccat tcaaactccg gaaagcagtt caaggagcag attcgtatcg
     5161 ccaaggaagt tatgcacaac agacagcttt cctctgatat caaatcactt gtcaagggtc
     5221 ttatgattga gagctatatt gaggagggca gccagaagat tggcgagcat gtctacggaa
     5281 aatccatcac cgatccttgt cttggatggg aggattcaaa gaagcttatc tacgatattg
     5341 cagaaatgaa ttcttaagca acaaactata tacatatttt tcacaaaata cgagccacag
     5401 aattttatat cctgtggctc gtattatttt gtctgtttat ttaatcatat tactataagc
     5461 aagaactgca ctgactgttt tggaatcctg tatttttccc gcataaatca tgtcgcacag
     5521 ctcctttagg gtgtatatct ctacatcgat tgactcggca tcatcgagat gctgctcgcc
     5581 ctttttcagg tttttggcaa gataaacatc tacttcttcg tcgcaaaagg ctactgttgt
     5641 gcgaagcgaa agtaaaaatg agatatcgtc agagctgtag cctgtctcct ccttaagctc
     5701 cctttctgca cacagccttg tgtcttctcc cacaaagtcc ctgcagccgg ccggaatctc
     5761 tattgtctct ctctcgaggg catttctgta ttggcgtacc atgattatcc tgccgtcatc
     5821 aagcaccgga agcactgccg cagcgccctt tctgtgtgag acaaaatccc actcctctgt
     5881 cttgccgttt gcaaactgca ttgtatcctt gtatatatcg agtattgagc ccttatatac
     5941 aagctctctt ttttgccgtt tcatttcatc aatcatttta ctgtttacct tctaaataat
     6001 taaagttcag agtctccatt ttgattagaa atcaacctct gtgtcataat agcagcactt
     6061 gagaagcttt tccatctcct cctggcttgg ctggcgcgga tttgagccgg tgcaggcatc
     6121 aaggatagca tttgccgcaa tctcaggaag tctctcaagg aatacctcct ccggtacaaa
     6181 gccctgctct gtcggataac tgtctgctcc gtaattcttg atgcagtgtg ggatattcag
     6241 atcatc
//
LOCUS       DPUF01000046            5202 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_2850, whole
            genome shotgun sequence.
ACCESSION   DPUF01000046 DPUF01000000
VERSION     DPUF01000046.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_2850"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            126..1451
                     /gene="rimO"
                     /locus_tag="DHV81_01625"
     CDS             126..1451
                     /gene="rimO"
                     /locus_tag="DHV81_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCI93233.1"
                     /translation="MKLLFISLGCDKNLVDSEYMIGMLANDGIEMTDDETQADIIIVN
                     SCCFIGDAKEESINTILEMAQYKETGKCKSLIVTGCLAQRYKDEIFKEIPEVDAILGT
                     NSYDTIVEAVHETLEKHRYSNLHTLEGLPSIKTKRIVTTGGHFAYLKIAEGCNKNCTY
                     CVIPSVRGRYRSVPMEDLIEQAKSLVEQGAKELILVAQETTLYGVDLYGEKSLHKLLD
                     ELNKISGLFWIRIMYCYPEEIYDELIESMIKDEKVCHYLDMPIQHCNDDILRRMGRKT
                     TKAELMERIRMLRERIPDITLRTTLICGFPGETQDNHEELMQFVNDMEFDRLGAFTYS
                     PEEGTKAAAMPDQIDEDIKADWQADVMELEEEVIFDKNETLKGKELYVFIEGKVADEN
                     AYIGRTYRDAPNVDGYIFINTDETLMTGDICKVMVTGAYEYDLIGELVK"
     gene            1448..1984
                     /gene="pgsA"
                     /locus_tag="DHV81_01630"
                     /pseudo
     CDS             1448..1984
                     /gene="pgsA"
                     /locus_tag="DHV81_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742518.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
     assembly_gap    1930..1948
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2005..2556
                     /gene="rsmD"
                     /locus_tag="DHV81_01635"
     CDS             2005..2556
                     /gene="rsmD"
                     /locus_tag="DHV81_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCI93234.1"
                     /translation="MRIIAGTARSLPLKTIEGKDTRPTTDKTKETLFNVMQFDVPGAY
                     FLDLFAGSGQIGLEALSRGAAYAVFVENSRKAASCIEDNIHFTKFDKVSRLMMTDAVT
                     AVRTLEGKYKFDIVFMDPPYNKELEKEVLITLSDSNILKDDTLIVVEASNDTDFDYLT
                     ELGYEIVKEKIYKTNKHVFIKRA"
     gene            2567..3052
                     /locus_tag="DHV81_01640"
     CDS             2567..3052
                     /locus_tag="DHV81_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HCI93235.1"
                     /translation="MKVGIYPGSFDPVTFGHLDIIERSAKIVDELVVGVLNNSAKNSL
                     FSLEERVSMIKEMTAHIPNVRVGCFEGLLVDYMKKINATIIIRGLRAVTDFEYELQIA
                     QANGVQNPEIETVFLTSSLNYSYLSSTIVKEFASYGGDISKFVPEQFIDRIYAKYNIT
                     R"
     gene            3066..3722
                     /locus_tag="DHV81_01645"
     CDS             3066..3722
                     /locus_tag="DHV81_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCI93236.1"
                     /translation="MSSKMEQIIEEIEDYIDGCKPSPISRNKILVNREQLEELLSELR
                     TKTPEEVKRYQKIISNKEAILADAQAKADQIIAQAQIQTNELVSEHQIMQQAYAQANE
                     VVQIATKQAQEILDSATNEANNLRMSAMAYTDSQLKSIEDILTGSIETSKARYDSLIS
                     SLQQFEDVVAANRAELFPPEENGDEAAGAESSDNEAASDTSDDDIEITNISDSDEDAK
                     "
     gene            complement(3702..4505)
                     /locus_tag="DHV81_01650"
     CDS             complement(3702..4505)
                     /locus_tag="DHV81_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93237.1"
                     /translation="MLWFNQIFPLLFIFTILSNLIISTNVLQNIPQKHIMLFTYIIGI
                     IFGFPIGAKLTADFCSKGYIDKNHREILSALANHFSLPFIITYALSEQLNICSHYSIY
                     LVSLYLPSAIGMIAMLSINKNRSLTQKIPAQGFKLNMQIVDAGIMKGFETLIKLCGYI
                     VLFSIVSRIPQTIAQKADCIMPLSADIIGAFFETTNGIGIVCGLCIPRTLSIVLCIAL
                     LSFGGVSCMVQTKSIFRDTGFRLYRYILLKAAMSILSCLLCIILFCILI"
     assembly_gap    4560..4607
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4667..>5202
                     /locus_tag="DHV81_01655"
     CDS             4667..>5202
                     /locus_tag="DHV81_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HCI93238.1"
                     /translation="MIRLDKFLSEMGLGTRSEVKKLIKTGQIAINGTVAAKPETKVDT
                     DADEVSVNGRIVKYQRYEYYLFNKPSGCVSATCDNVHDTVMDYITDAVHDDLFPVGRL
                     DIDTEGLLLITNDGALSHELLSPSKHVAKTYYARIDGEVTLDDIIRFKEGIDIGEERP
                     TKPAELVIKKSGSVSEIEL"
BASE COUNT         1618 a          889 c         1234 g         1394 t
ORIGIN      
        1 acgggagctg tcagaatcgt ctcagatgat gaggatttaa acgaaggcga agaggcttaa
       61 tctaggaggg gaaaggctgt tttgtctttc cctttaattg ttaatactta catttgagag
      121 gtactatgaa attattattt atttcgcttg gatgcgataa aaatcttgtg gattcagagt
      181 atatgatagg catgcttgca aatgacggca ttgagatgac cgatgatgag acgcaggctg
      241 atatcattat cgtaaacagc tgctgcttca taggtgatgc aaaggaggag agtatcaata
      301 caattctcga gatggctcag tataaggaga caggcaaatg caaatcactt attgtgacag
      361 gctgtctggc acaaagatac aaggatgaga tttttaagga aatcccagag gttgatgcca
      421 tacttggcac caattcatat gatactatag ttgaagctgt ccatgagaca cttgaaaaac
      481 acagatacag caatctgcac accttagagg ggcttcccag cataaagaca aagcgtattg
      541 tcaccacagg agggcatttc gcttacctta agatagcaga ggggtgcaat aaaaactgta
      601 cctactgtgt cattccatct gtcagaggcc gttacagaag tgtgccgatg gaggatttga
      661 ttgagcaggc gaagagcctt gtggagcagg gagcaaagga gcttatactt gttgcccagg
      721 agaccacact ttacggagtg gatttgtatg gtgagaagtc acttcataag cttttggatg
      781 agctcaacaa gataagcggg ctgttctgga taaggattat gtactgctat cccgaggaga
      841 tatacgatga gcttatagag tccatgataa aggatgagaa ggtatgccat tatctggata
      901 tgccaatcca gcactgcaat gacgatattt taaggcgcat gggtagaaaa acaacaaagg
      961 ctgagcttat ggagcgtatc agaatgctgc gtgagagaat acctgatatc acactaagga
     1021 ccacacttat atgtggcttc ccgggtgaga cacaggataa tcacgaggag cttatgcagt
     1081 ttgtaaatga tatggagttt gaccgtctgg gagcctttac gtattccccg gaggagggca
     1141 caaaggcagc tgcaatgcct gatcagatag atgaggatat aaaggctgac tggcaggcag
     1201 atgtcatgga gcttgaggaa gaggtcattt ttgataaaaa tgagacacta aagggtaaag
     1261 aattatatgt tttcatagag ggcaaggtgg ctgatgaaaa tgcttatatc ggccgtacat
     1321 acagagatgc accgaatgtt gacggatata tattcatcaa cactgatgag acacttatga
     1381 ccggcgatat atgcaaggtt atggtgaccg gtgcgtatga atatgatttg ataggagaac
     1441 ttgtaaaatg aatttaccaa acaaacttac aatcttaaga gtgatactta ttccgttttt
     1501 tgtatttttc cttatttcac cgttttttga cggatacgga aattatatag cacttgcgat
     1561 atttatcatt gcaagtctga ccgatatggc tgacggaaaa attgcaagaa aatacaatct
     1621 tgttacaaat ttcggcaagt ttatggatcc gcttgcagac aagctgcttg tatgctctgc
     1681 aatgatatgc cttgttgatt taaagcttat ccctgtatgg gttgtgctta ttatcattgc
     1741 aagagagttt atcataagcg gcttcagact cgttgcatca gacaatggaa ttgtcattgc
     1801 agcaagctac tggggaaagt ttaagacaac cttccagatg cttatggtta ttgttattat
     1861 ttttaatatc aatctgcagc ttggctggtt aaatatcctt ggaacaatcc tcatttatgt
     1921 ggcacttgtn nnnnnnnnnn nnnnnnnngc aaagaataag gacgtcttaa aggaccagaa
     1981 atagactata acagggagaa aagtatgaga ataatagcag gaacggcgag aagcctccct
     2041 ctaaagacta ttgagggaaa agatacaaga ccgactactg acaagacgaa ggagactctt
     2101 tttaatgtca tgcagtttga tgttcccgga gcatattttt tagatctgtt tgcgggaagc
     2161 ggacagatag gacttgaagc gttaagccgc ggtgcagcct atgctgtatt tgtggagaat
     2221 tcaagaaaag ccgcttcatg catcgaggac aatatccatt ttacaaagtt tgataaggtc
     2281 tcaaggctta tgatgacgga tgctgtcacg gcagtacgta cgcttgaggg caaatacaaa
     2341 tttgatatcg tgtttatgga tccgccatac aataaggagc ttgaaaagga agtgcttatt
     2401 acactttcgg acagcaatat attaaaggat gatacactta tcgtggttga ggcttcaaac
     2461 gatactgatt ttgattatct cacagagctt ggctatgaga tagtcaaaga aaaaatttat
     2521 aagacaaata agcatgtttt cataaagcgt gcataggaga tacaatatga aggtaggaat
     2581 ttatccggga agctttgatc cggtgacatt tggacacctg gatataatag agcgttcagc
     2641 caaaatagtt gacgagctgg ttgtcggagt actcaataat agtgcaaaaa actcgttgtt
     2701 ttctttggaa gaacgtgtta gtatgataaa ggaaatgaca gcacacatac caaacgtcag
     2761 ggtagggtgc ttcgaaggac ttttagttga ctatatgaag aaaatcaacg caacaatcat
     2821 aataagagga ttgcgtgcag tgacagattt tgagtatgag ctgcagattg cacaggctaa
     2881 cggagtgcag aatccggaaa tagagacggt ttttctcacc tcgagcctca attattcata
     2941 tttaagctct acaattgtca aggaatttgc atcatacgga ggagatatat caaagttcgt
     3001 acctgaacag ttcattgata gaatttatgc caaatacaat attaccaggt aaggagacag
     3061 tatttatgag cagtaaaatg gaacagatta ttgaagaaat cgaagactat attgatggct
     3121 gcaagccgtc acctatttca agaaacaaga ttcttgtcaa tcgtgaacag ttagaggagc
     3181 ttctttcaga gcttcgtaca aagacccctg aggaggtcaa gagatatcag aagattatca
     3241 gcaataagga ggctattctt gctgatgcac aggcaaaggc tgatcagata atcgcgcagg
     3301 ctcagattca gacaaatgag cttgtcagcg agcatcagat tatgcagcag gcatatgctc
     3361 aggcgaatga ggtagtgcag attgcaacaa agcaggcaca ggagatactt gacagtgcca
     3421 caaacgaagc aaataatctc agaatgagtg ccatggcata tacggacagc cagctcaaga
     3481 gcattgagga catcttaaca ggctctattg agacttcaaa ggccagatat gacagcctta
     3541 taagttcact gcagcagttt gaggatgttg ttgcagcaaa cagagcagag cttttcccac
     3601 cggaagagaa tggcgatgaa gctgcaggag cggagtcgtc tgacaatgag gcagcttcag
     3661 atacatctga tgatgacata gaaatcacca atatttccga ttcagatgag gatgcaaaat
     3721 aggataatac ataacaggca tgagagtatg ctcatggctg ccttaagcag tatgtatctg
     3781 taaagcctaa agcctgtatc ccggaatatt gatttggtct ggaccatgca tgatacaccg
     3841 ccaaatgaca gaagtgcaat gcacagcact attgacaggg tgcgtggaat gcatagaccg
     3901 cagactatgc cgataccgtt tgtggtttca aaaaaggcac ctattatgtc tgccgacagt
     3961 ggcatgatgc agtcagcctt ttgggcaatg gtctgtggga tacgtgagac tatagagaac
     4021 aatacaatat atccacagag ttttataagg gtttcaaacc ctttcattat tccggcatca
     4081 acgatttgca tgttaagctt aaatccttga gccgggattt tttgtgttaa gcttctgttt
     4141 ttgttaatcg aaagcatagc tatcattcct atagcagatg gcagatacag tgacacaaga
     4201 taaatgctgt agtggctgca tatgttaagc tgctcggaca gtgcataggt aatgataaag
     4261 ggaaggctga aatggtttgc cagtgcactc agtatttccc tgtgattttt atctatgtat
     4321 cctttcgaac aaaaatctgc tgtgagcttt gctcctatcg gaaatccaaa tattatgcct
     4381 attatgtatg taaaaagcat gatatgcttt tggggaatgt tttgcagcac atttgttgag
     4441 ataatcaggt tagataaaat cgtaaatata aacagaagcg gaaatatctg gttgaaccat
     4501 agcattatgc cagctttagc gcacatactt acctgtgccg gaaacaatat gtaataaatn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt aattttttaa
     4621 gtttgttttt actcatgtga cagtatatgt ggagagaaat atcaatatga ttcgattaga
     4681 taaattttta tcagagatgg gacttggcac acgctctgag gtaaaaaagc ttataaagac
     4741 cggacagata gccataaatg gcacggtggc cgcaaagcct gagacaaagg tagatactga
     4801 tgccgacgag gtatcagtta acggcagaat agttaagtat caaaggtatg agtattacct
     4861 gtttaataag ccctcaggct gtgtttctgc gacctgtgat aatgtgcatg atacagtcat
     4921 ggactatatc acggatgctg tgcatgatga tctgtttcct gtcggaaggc ttgatataga
     4981 cacagagggg cttttactta tcacaaatga tggagcactt tcgcacgagc tcttaagtcc
     5041 ttcaaagcac gtggcaaaga cttactacgc acgtattgat ggtgaggtga ctttggatga
     5101 tatcatcagg tttaaagagg gcattgatat aggcgaggaa agacctacaa agcctgcgga
     5161 gcttgttatt aaaaaaagtg gcagcgtatc cgagattgag ct
//
LOCUS       DPUF01000047            7154 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10015, whole
            genome shotgun sequence.
ACCESSION   DPUF01000047 DPUF01000000
VERSION     DPUF01000047.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7154)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10015"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="DHV81_01660"
     CDS             <1..258
                     /locus_tag="DHV81_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCI93239.1"
                     /translation="GNELFYYTFFNESSRHNYEIDFLIAKKNKVCPIEVKSSGYKTHK
                     SLDEFSIKFSNRILQKYLVYTKDFSKDKDILCLPAYMISFL"
     gene            315..1070
                     /gene="xth"
                     /locus_tag="DHV81_01665"
     CDS             315..1070
                     /gene="xth"
                     /locus_tag="DHV81_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCI93240.1"
                     /translation="MKFISWNVNGLRACVQKGFLDFFNSIDADFFCIQESKLQAGQID
                     LDLPGYHQYWNYAEKKGYSGTAIFAKNKALSVSYGIGIEEHDKEGRVITLEYDNFYLV
                     TCYTPNSQNELKRLPYRMQWEDDFREYLKTLDAKKPVVLCGDLNVAHNEIDLKNPKTN
                     RKNAGFSDEERAKMTELLGSGFTDTFRYFYPDAEGIYSWWSYRFKAREKNAGWRIDYF
                     ITSKRINDKLKKAAIHTDVLGSDHCPVEVDIEF"
     gene            complement(1089..3335)
                     /locus_tag="DHV81_01670"
     CDS             complement(1089..3335)
                     /locus_tag="DHV81_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCI93241.1"
                     /translation="MGNSYKNCKIQDNTAELIYENGSLFVTIFENNIVHVAQKHGIES
                     VAIEEDFIPKTATPDITCKDTSDVKGTAAEAGVSDAAVKAVISARDITVNVKDNEKLD
                     IYYKGKLVLSDYETARKKSEKNPYEDLAIAELEGHTVGKDEEKTDAVTIIKKLGKDDA
                     IYGLGDKPGCLNKRGYSYVNWNTDDPAPHVDSFKSLYKSIPFFIVLGDEYCYGIFADN
                     TYKTTFDFGYENTDYYFVEHEKGELDYYFMPGNDMAEVVGLYTSLTGTTPLYQRWIYG
                     SHQSRWGYYTQDEVLDIADKFRELDIPCDVIHMDIDYMNGYRVFTFDDKKFPDVKGLS
                     EKLADRGVKLISIIDPGVKKDEDYFMYKEGMEMDAFAHDTDGSVYENAVWPGTSVFPD
                     FTKQSVRSWWGDKTKILLEHGISGIWNDMNEPASFNGPLPDDVQFEYGAHEKVHNIYG
                     HFMAKATYEGLAKNDGGKRPFVLTRAAYAGSQKYCGGWTGDNHSIWAHIALSLEQVCN
                     LSVSGLAMCGSDIGGFGSDTTPELLVRFYEAAVFVPFFRNHSAMGTRRQEPWQFDETT
                     IDAVRKTVKLRYRFIPYIYDLAHECEKTGAPIVRPLVYEYHADKHVRNISDEYMLGSF
                     VLVAPVIAPGKEAREVYLPDGDWYDYYTGEKYSGGRYILADAPLDKVPVFIKAGAIIP
                     VADGEIRSTEDITEDKISILTYPGKGSFVHYQDDNETFAYRNGAYNAVEYTLDGDKLE
                     KKVLHKGL"
     gene            complement(3477..5870)
                     /locus_tag="DHV81_01675"
     CDS             complement(3477..5870)
                     /locus_tag="DHV81_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCI93242.1"
                     /translation="MQEIYRVKTSPVALSDNMIIGEKYRITLLTEALVRLEYSEDGVF
                     EDRATQTVLFRDFPKTGYHVVRNADGIEVHTSMLHLIYNEKEFSSHGLSIQIKGNLSA
                     YHSIWHYGEKVHDLQGTARTLDDVNGEVELGHGVVSRFGYSILDDSKSQILLDDGWIE
                     PRKKGVSDLYFFGYGHDYKQALNDFYRLCGKTPMLPRYALGNWWSRYYKYSEESYMEL
                     MDKFDEKNIPFTVAVIDMDWHQVDVDPKYGSGWTGYTWNKKLFPDPKRFLGKLHDRGM
                     HTTLNVHPADGVQGCEEMYVDMAKEMGVDYENEDPVVCDPASPKFMDAYFKYLHHPRE
                     AEGVDFWWIDWQQGSNCKVEGLDPLWIFNHFHYLDNKRDGKRPMTFSRYAGPGSHRYP
                     IGFSGDTHITWESLDFQPYFTTTATNIGYGWWSHDIGGHMLGYKDDELTARWTQYGIF
                     SPIMRLHSSCSEFNGKEPWRFKKETEEAMEAALRQRHCMIPYLYTMNYRSYAENMPLI
                     EPMYYEYPENAEAYEVKNQYFFGSQLMVAPVTTPRIKGLNVAKTKVWFPEGIWYDIYT
                     GMRYDGGRMLEVYRDLSSIPVFAKAGGILVCADADELDARSVIKNPDVLCVRVFPEAD
                     GEFELYEDDNESCGYVDGRCVTTAMKYSADKDMFVISPADGDTSLIPDNRTYKLVFER
                     RTEAAADKVKVSVDGKEVLAKNKYDKENRKLIVTVTASTASKISVAISKDTVALDNQI
                     EKRCFDFLNQAEIGFVLKDEIYGLITSGKKTTVILSELKAKELDTELYGVLTEILTA"
     gene            complement(5898..6719)
                     /locus_tag="DHV81_01680"
     CDS             complement(5898..6719)
                     /locus_tag="DHV81_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI93243.1"
                     /translation="MRKKVSSIVLNVFLLLLGIVTVYPFIWMLCSSFKQNKEIMALQQ
                     HLLPQEFTIDNYISMNSHFNFMRFFGNSLFITIVVTLLVVYTSTICGFVLSKYKFKGR
                     NILFGFVLATMMIPWCVTIIPKYTMIQKFGWMDSYKALIIPAMFSGFGIFMMKQHIET
                     IPDEIIEAARMDGANEFYIFHRIIFPMAKNGISSIAIFQFLWTWEDYLWPYLVINTKE
                     KQLLAVGLKMFNGQYSTDYGALFAATAISIIPVLLIYLFFQKQFIAGIAASAVKG"
     gene            complement(6732..>7154)
                     /locus_tag="DHV81_01685"
     CDS             complement(6732..>7154)
                     /locus_tag="DHV81_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI93244.1"
                     /translation="MNPKTALIAVIIMTIWKDFGYAVVMYMAGLYSLPADVMEAAQVD
                     GASGWQKFRYITLPLLKPMTLFVVITSIISYIQAYVQILIMTEGGPGTSTYLASYIIY
                     DEAFVKYNFGYASALSFVLFVITAVLTVLSFKVSGKEE"
BASE COUNT         1963 a         1736 c         1289 g         2166 t
ORIGIN      
        1 ggcaacgaat tattttacta tacttttttt aatgaaagtt cacgccataa ttacgaaata
       61 gattttctta ttgccaaaaa aaataaggtt tgccctattg aagtaaaatc cagtggctat
      121 aaaacccata agtcactgga tgaattttca ataaaatttt caaaccgtat tcttcaaaaa
      181 tatctcgtat atacaaagga tttctcaaaa gacaaagaca tactctgtct gcctgcttac
      241 atgatttcgt ttttgtaaga agaatacttt ttaaatatga ttttaataca cacacttata
      301 tggaggacta agacatgaaa tttatatcgt ggaatgtaaa cggactgcgc gcatgcgtcc
      361 agaaaggttt ccttgatttt tttaacagta tagatgcgga tttcttttgt atacaggagt
      421 ccaagctaca ggcgggacag attgaccttg acctgccggg ctatcaccag tactggaatt
      481 acgccgagaa aaaaggctat tccggcacag ccatttttgc caaaaacaag gcattatcag
      541 tgtcttacgg cataggcatt gaggagcatg ataaggaagg acgtgtcatc acactcgaat
      601 atgacaattt ctatctcgta acctgctata caccaaactc acagaatgag ctgaaaaggc
      661 tgccgtaccg tatgcagtgg gaggatgatt tcagagaata tcttaaaaca ctcgatgcca
      721 aaaagcctgt cgtactgtgt ggtgatctga acgtcgcaca caatgaaatc gacctgaaaa
      781 atccaaaaac aaaccgcaag aatgcaggtt tttcagatga ggaacgtgct aaaatgacgg
      841 agcttttagg ttccggcttt accgatacct tcagatattt ttatccagat gccgagggta
      901 tatattcctg gtggtcatac cgttttaagg ctcgcgagaa gaacgcaggc tggcgtatcg
      961 actactttat cacatcaaag cgcatcaatg ataagcttaa aaaggcagca atccataccg
     1021 atgtgctcgg ttcagaccac tgccctgttg aggttgatat tgaattttaa atttaaggtt
     1081 atgtattttt acaatccctt atgcaacact ttcttctcga gcttatcccc atccagtgta
     1141 tactcaacag cattgtacgc accgtttctg tatgcaaacg tctcattgtc atcctgataa
     1201 tgcacgaagc tgcccttgcc cggatatgtc agtatgctga tcttatcctc tgtaatgtcc
     1261 tctgtagagc gaatttctcc gtctgcaaca gggataatgg ctcctgcttt tatgaagaca
     1321 ggtactttat caagtggtgc atctgctaaa atatatctgc cgccactgta tttttcgcct
     1381 gtgtagtaat cataccagtc gccgtcaggc agataaacct ctcttgcctc cttgcccggt
     1441 gcgatcacag gtgcaaccag tacaaagctt ccgagcatat attcatcgga aatatttctc
     1501 acatgcttat ctgcatgata ctcgtatacg agcgggcgca cgataggtgc tcctgtcttc
     1561 tcacactcat gtgccagatc atagatatat gggataaatc tgtatctgag ctttactgtc
     1621 tttctgactg catctatcgt agtctcgtca aactgccaag gctcctgtct gcgtgttccc
     1681 atcgctgaat gatttctgaa aaacggcaca aatactgctg cctcataaaa tctgacaaga
     1741 agctccggtg tagtatcact tccgaaaccg cctatatcgc tgccacacat ggcaagtccg
     1801 ctgactgaca gattgcagac ctgctcgagc gaaagtgcta tatgcgccca gatgctgtga
     1861 ttgtctccgg tccagccacc gcagtatttc tggcttccgg catatgcagc ccttgtaagt
     1921 acaaacggac gttttccacc atcatttttc gcaagtccct catatgtagc ttttgccata
     1981 aaatgtccgt aaatattgtg aaccttttca tgtgctccat actcaaactg cacatcatcc
     2041 ggcagtggtc cgttgaagct tgcaggctca ttcatatcat tccatatgcc actgataccg
     2101 tgctcgagaa gtatcttggt cttgtctccc caccagcttc tgacgctttg ctttgtgaag
     2161 tccggaaata ccgatgttcc cggccagaca gcattttcat atacactgcc atctgtatca
     2221 tgtgcaaatg catccatctc catgccctct ttgtacatga aataatcctc atcctttttc
     2281 acacccgggt caatgatgga tatgagcttt acacccctgt cagcaagctt ctctgaaagc
     2341 ccctttacat ctgggaattt cttgtcatca aaggtgaata ccctgtagcc gttcatgtaa
     2401 tcgatatcca tatgtatcac atcgcatggg atatcaagtt cgcggaattt gtcagctata
     2461 tcaagtacct catcctgtgt gtaatagccc cagcggcttt gatggctgcc gtatatccat
     2521 ctctgataaa gcggtgtggt gcctgtcagt gatgtataaa gtcctaccac ctctgccata
     2581 tcatttcccg gcataaaata gtagtccagc tcgcccttct catgctcgac gaaatagtaa
     2641 tctgtgtttt cataaccgaa atcaaaggtt gtcttgtagg tattatccgc aaaaattcca
     2701 tagcaatatt catcgcccaa cacgataaaa aacggtatgg atttgtacag cgatttgaag
     2761 ctgtccacat gtggcgcagg atcatccgtg ttccagttta catatgaata tccacgcttg
     2821 ttgaggcatc ccggcttatc tcccagaccg taaatagcat catcctttcc aagctttttg
     2881 atgatggtaa cagcgtcagt tttttcttca tcttttccga cagtatgtcc ctcaagctct
     2941 gcgatggcaa gatcctcgta cggattcttc tcactctttt tgcgtgctgt ttcataatcc
     3001 gaaagcacaa gcttcccttt gtaataaata tcaagcttct cgttatcctt tacatttaca
     3061 gtaatatccc ttgcacttat gactgcttta actgctgcat cgcttacacc ggcttctgct
     3121 gcggtcccct tgacatcaga cgtatccttg caggttatgt ccggtgtggc ggtcttcgga
     3181 ataaagtcct cttctattgc aacactttcg atgccgtgtt tttgtgcaac atgcactatg
     3241 ttgttttcaa atatggtaac aaacagactg ccgttctcgt agatcagctc tgctgtgttg
     3301 tcctgtattt tacagttctt atatgaattt cccattgcat aatcctccat tgaattatat
     3361 agtttctcct gatgtttctt ataaaaatac ttccgtttac tataatagta tcatccattt
     3421 ttaattttta caacaaagaa agtcaccggt cacacctagt atgaccggtg actgcctcag
     3481 gctgtaagta tctctgtcaa tacaccatac agctctgtat caagctcctt tgccttgagc
     3541 tctgataaaa tcactgttgt tttctttccc gatgtaataa gaccgtagat ttcatccttt
     3601 agcacaaagc ctatctctgc ctgattcagg aaatcaaagc atcttttttc gatttgatta
     3661 tcaagcgcta ctgtgtcttt cgatattgca acgcttattt tgcttgctgt cgatgcagtt
     3721 acagtaacga tgagctttct gttttcttta tcatatttat tctttgccaa tacctcttta
     3781 ccgtccacag atacctttac cttatcggct gctgcctcgg ttcttctttc aaagacaagc
     3841 ttataggttc tgttgtcagg tattaatgat gtatctccat ctgccggaga aatgacaaac
     3901 atatctttat ccgcagaata tttcatagct gtggtcacac atctgccatc cacatatccg
     3961 caggattcat tgtcatcctc gtatagctca aactcaccat cagcctccgg gaataccctg
     4021 acacataata catcaggatt tttgataaca gatcttgcat caagctcatc agcatctgca
     4081 cacacaagta tgcctccagc cttcgcaaac acaggtatgc tgctcaaatc cctgtaaacc
     4141 tccagcattc ttccaccatc atagcgcatg cctgtataga tatcatacca gatgccctcc
     4201 gggaaccata ctttggtttt tgccacatta agtcccttaa tacgtggagt tgtaacaggt
     4261 gctaccataa gctggctgcc aaagaaatac tgattcttta cctcataagc ctctgcattt
     4321 tcaggatact cataatacat aggctcaatc agcggcatat tctcggcata gctgcgatag
     4381 ttcatggtat acagatacgg aatcatacaa tgcctctgtc tgagcgccgc ctccatcgct
     4441 tcctcagtct cttttttaaa tcgccacggc tccttgccgt taaattcgct gcatgagctg
     4501 tgcagacgca ttatcggaga gaatatacca tattgtgtcc atctggctgt cagctcatca
     4561 tccttgtagc caagcatatg tccaccaata tcatggctcc accatccata tccgatgttt
     4621 gtcgctgtcg ttgtaaaata cggttgaaaa tcaagcgact cccatgtaat atgtgtatct
     4681 cccgaaaatc caatcggata tctatggctt cccggacctg catagcgcga aaatgtcatt
     4741 ggacgcttac cgtctctttt attatcgaga taatgaaaat gattaaaaat ccatagcgga
     4801 tccaatcctt caaccttgca gttgctgccc tgctgccagt caatccacca gaaatccaca
     4861 ccttcagcct ctctcggatg atgaagatac ttaaaatatg catccataaa ctttgggctt
     4921 gccggatcac atacaaccgg gtcctcattt tcatagtcca cgcccatttc ctttgccata
     4981 tcaacataca tttcttcaca gccctgcaca ccatccgcag gatgtacgtt taaggtagta
     5041 tgcattcctc tgtcatggag ctttccaaga aagcgcttcg gatccgggaa aagctttttg
     5101 ttccaagtat atcctgtcca gccgcttccg tatttagggt ccacatcaac ctgatgccag
     5161 tccatatcta ttactgctac cgtgaatggg atattctttt catcgaattt gtccatcagc
     5221 tccatatagc tttcttctga gtatttataa tatctgctcc accagtttcc cagtgcatat
     5281 cgcggaagca tcggagtctt gccgcaaagc ctgtaaaaat cattgagcgc ctgtttataa
     5341 tcatgtccat atccaaagaa atacaaatca gacacacctt tttttcttgg ctcaatccat
     5401 ccatcgtcaa gtaaaatctg tgatttactg tcatcaagaa ttgaatatcc aaagcgtgaa
     5461 acaacaccgt gtccaagctc aacctctccg tttacgtcat caagtgtacg cgctgtaccc
     5521 tgcaaatcat gcaccttctc gccataatgc cagatgctgt gatatgcgct caggtttccc
     5581 ttaatctgta tactcagtcc gtgtgatgag aactccttct cattatatat caggtgcagc
     5641 attgatgtat gaacctcaat tccatcagcg ttacgcacca catgatagcc ggtctttgga
     5701 aaatccctga acaatactgt ctgtgttgcc ctatcctcga atacgccatc ttcactgtat
     5761 tccagacgga caagcgcctc tgtcagtaat gtaatcctgt atttttcacc tattatcata
     5821 ttgtccgata atgccaccgg acttgtcttt actctgtata tttcctgcat gaaatactcc
     5881 ctctcatata aattacgcta tcccttgaca gccgatgccg caattcctgc gataaactgc
     5941 ttctggaaga acagatatat cagtaaaacc ggtatgatag atattgccgt tgccgcaaaa
     6001 agtgcgccat aatctgttga atactgtccg ttaaacatct taagaccaac tgccaatagc
     6061 tgcttttcct tggtattaat tacaagataa ggccacagat agtcttccca tgtccacagg
     6121 aactgaaaaa tagctatact tgaaattccg ttttttgcca tcggaaatat aatcctgtga
     6181 aagatataaa actcattagc gccatccatt ctggcagctt caataatctc atcagggatg
     6241 gtctcaatgt gctgcttcat cataaatatt ccaaatccgc tgaacatagc cggaataata
     6301 agtgccttat agctgtccat ccatccgaat ttctgaatca tagtgtattt tggaataatt
     6361 gttacacacc atggaatcat catagtagcc aatacaaagc caaataggat atttctgccc
     6421 ttaaacttat atttgcttaa tacaaaacca cagattgtac tggtgtatac cacaagcagg
     6481 gttacaacaa tcgtgataaa caaactgttt ccaaaaaacc tcatgaaatt gaaatgcgaa
     6541 ttcatgctga tgtaattgtc tattgtaaac tcttgtggaa gcagatgttg ctgcaacgcc
     6601 attatttcct tattctgctt aaatgatgag catagcatcc atataaacgg ataaactgta
     6661 acaatgccca ataatagaag gaatacattt aaaactatac tgcttacttt ttttctcatg
     6721 atattctcct cctactcttc cttgcccgat actttgaaag aaagcacggt aagcactgcg
     6781 gtaatcacaa aaagcacaaa tgaaagtgct gatgcatatc caaagttgta tttgacaaag
     6841 gcctcatcgt aaataatgta agaagccaga tatgttgatg ttcccggtcc accctctgtc
     6901 atgatgagta tctgaacata tgcctggata tacgaaataa ttgatgtaat gactacaaaa
     6961 agtgtcatag gcttaagcaa cggcaatgta atgtatctga atttctgcca gccactcgct
     7021 ccgtcaacct gtgctgcctc catgacatct gccggaagtg agtacaggcc tgccatatac
     7081 atgacaaccg catatccaaa atccttccag atggtcatta taattactgc aatcagagca
     7141 gttttgggat tcat
//
LOCUS       DPUF01000048            7610 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_9382, whole
            genome shotgun sequence.
ACCESSION   DPUF01000048 DPUF01000000
VERSION     DPUF01000048.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_9382"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /gene="recN"
                     /locus_tag="DHV81_01690"
     CDS             complement(<1..1535)
                     /gene="recN"
                     /locus_tag="DHV81_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCI93245.1"
                     /translation="MLQNLHVKNLALIDECEVEFSDGLNILSGETGAGKSIIIGSINL
                     ALGEKVQKEMLRDNEKPAFVELIFSVEDPKIIEALRELDVEVEDGCVILSRKITQSRA
                     VGRVNGEAVSVSRMKEIAAYLIDIHGQHEHQSLLSKKKHLDILDEYAKQPLGDKKQQL
                     SVTYKAYRALKDEYEKSNIDNEERSRELSFLEYEVKEIEEASLVSGEDEELEAQFRKF
                     SNGKKIMEGVNAAYSATGGEMESASELIGRAVRELSQVSGYDEDVEALESQLSEIDSL
                     LSDFNHEISGYISQAEFDEETFYETQKRLDEINHLKSKYGNSIDDILIALNEKRERIS
                     VLNDYDSYLQKLEQQLAKKEKELAQISDEVSEIRQRSAVKLVSEIKSALNDLNFLDVQ
                     FDMQFDRLPDYTANGIDAPEFLISTNPGEPLKPLGKVASGGELSRIMLGIKTIMAEND
                     HIESLIFDEIDSGISGRTAQMVSEKMNELGRNHQIICITHLPQIAAMADAHFLIEKAV
                     ENKS"
     gene            complement(1617..2066)
                     /gene="argR"
                     /locus_tag="DHV81_01695"
     CDS             complement(1617..2066)
                     /gene="argR"
                     /locus_tag="DHV81_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCI93246.1"
                     /translation="MKTNRQSKIIEIIQKNEVETQDELSALLEKDGFRVTQATVSRDI
                     RELKLTKIPTAGGRQKYAVITDAPENLSKKYERVLREGFLSMDMAQNILVIKTVSGMA
                     SAVCAAIDAMKMREIVGSIAGDDTIMCAIRTVDDTYAVMKKIRRIVE"
     gene            complement(2125..2983)
                     /locus_tag="DHV81_01700"
                     /pseudo
     CDS             complement(2125..2983)
                     /locus_tag="DHV81_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516683.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
     assembly_gap    2452..2549
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2987..3793)
                     /locus_tag="DHV81_01705"
     CDS             complement(2987..3793)
                     /locus_tag="DHV81_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HCI93247.1"
                     /translation="MKERLDVLLVNRGLAPSREKAKTMIMEGNVFVNNNREDKAGSTF
                     PDDCNIEIHGKTLQYVSRGGLKLEKAMKHFDITMDGKVCMDIGASTGGFTDCMLQNGA
                     KKVYAVDVGYGQFAWKLRQDERVVCMEKTNIRYVTPNDIDDELDFASVDVSFISLTKV
                     LGPARALLKDGGEMVCLIKPQFEAGREKVGKKGVVRDKSVHEEVVNNIISFALSNGFS
                     VLDLEYSPIKGPEGNIEYLVHIKKTDDPIKEESVDIHSVVEAAHGELDRK"
     gene            complement(3812..5668)
                     /gene="dxs"
                     /locus_tag="DHV81_01710"
     CDS             complement(3812..5668)
                     /gene="dxs"
                     /locus_tag="DHV81_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCI93248.1"
                     /translation="MVLEKIQKENDIKKLTPEELELLKDEIRQFLIESISVTGGHLAS
                     NLGVVELTMALHLCFNLPKDKIVWDVGHQSYTHKILTGRKDGFSSLRQYGGMSGFPKA
                     DESDCDCFNTGHSSTSISAGLGLATARQVTGEDYHVVSVIGDGALTGGMAYEALNNAS
                     SVKGNFIIVLNDNNMSISENVGGISQYLSGFRTADAYRDLKNNVMNSLNHIPIYGERM
                     VKHIRNTKSSIKQLFIPGMFFEEMGIIYLGPVDGSDIKEMCHVFDEAKRVDGPVLVHV
                     LTKKGAGYGPAERYPSRFHGAEPFVIETGLPKNKRTKANYTDVFSTVMKKLGERNPKV
                     VAITAAMADGTGLRRFHRNFPDRFFDVGIAEAHATTFAAGLAKAGLIPVFAVYSSFLQ
                     RAFDQILHDVCIQNLHVIFAIDRAGLVGSDGETHQGIFDISYLSVIPNMTIMAPKNKW
                     ELSDMMKFAVAYDGPIALRYPRGAAYDGLKEIRQPIELAKSELIRKGSTVAIMALGSM
                     VKTAVDVVKLLEAEGISATLINARFAMPFDKKAIKELPSEHSLLVTMEENVQSGGFGE
                     HVTEYVKTNGIALEVLTVALPDCYVEHGNVEVLKKELHVDAESVAKRIIAAL"
     gene            complement(5670..6593)
                     /locus_tag="DHV81_01715"
     CDS             complement(5670..6593)
                     /locus_tag="DHV81_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCI93249.1"
                     /translation="MSLNGNLNKSQFMEELQQKVEHINDVLEKFLPAEEGQQRIIFEA
                     MNYSVRAGGKRLRPILMEETYHMFGGSSAVIEPFMAAIEMIHTYSLVHDDLPAMDNDE
                     YRRGKKTTHAVYGEAMGILAGDALLNLAYETAAKAFDMEVADTRVARAFAVLAKKAGV
                     YGMVGGQVVDVESEKSDDCSITREKLDFIYRLKTGALIESSMMIGAILAGASSDEVSR
                     VEQIAAKLGLAFQIQDDVLDVTSTLEVLGKPVGSDEKNNKATYVTFEGLDKAVSDVER
                     ISKEAEEQLDDLGYDDAFLKELFEYLIHREK"
     gene            complement(6593..6811)
                     /gene="xseB"
                     /locus_tag="DHV81_01720"
     CDS             complement(6593..6811)
                     /gene="xseB"
                     /locus_tag="DHV81_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCI93250.1"
                     /translation="MSKENEKTLTLEERFAHLEQIVARMEDADVSLDEAFELYKKGLD
                     EVKSANDMVQGMEKAMLVLNSDGQLEEF"
     gene            complement(6795..7610)
                     /locus_tag="DHV81_01725"
                     /pseudo
     CDS             complement(6795..7610)
                     /locus_tag="DHV81_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743047.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
     assembly_gap    7267..7338
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1763 a         2081 c         1343 g         2253 t
ORIGIN      
        1 gatttgttct cgactgcttt ttctataagg aaatgcgcat ctgccatagc tgctatctgc
       61 ggcagatgtg tgatacatat aatctgatgg ttgcggccaa gctcattcat cttctcagag
      121 accatctgag ctgtacgtcc actgattcca gaatcaatct catcgaaaat aagcgactca
      181 atgtggtcat tttctgccat gattgtcttg attccaagca tgatacgcga cagctctcct
      241 cccgatgcta ccttgccgag cggcttaagc ggctctccgg ggttagttga tatgagaaac
      301 tccggcgcat cgatgccgtt tgccgtatag tccggcaatc tgtcaaactg catatcaaac
      361 tgtacatcaa ggaaattcaa atcattaagt gctgatttaa tttctgacac aagctttaca
      421 gcactccttt gtctgatttc tgatacctca tccgatatct gtgccagctc cttttccttt
      481 ttcgcaagct gctgctctaa tttctgtaaa taagaatcat aatcattcag gacactgata
      541 cgctccctct tttcattgag tgctatcagt atatcatcaa ttgagttgcc gtatttgctc
      601 tttagatgat ttatctcatc cagacgcttt tgtgtctcat agaaggtctc ctcgtcaaac
      661 tcagcctgtg atatatatcc tgatatctcg tgattaaaat cagaaagcag actgtctatc
      721 tctgacagct gactctccag agcttccaca tcctcatcat aacctgatac ctgcgacagc
      781 tctcttaccg ccctgccaat aagctctgag gcactctcca tctcgcctcc tgtggcagaa
      841 tatgcagcat ttacaccctc catgattttc tttccgtttg aaaacttcct aaactgcgcc
      901 tcaagctcct catcctcacc agacaccagg gaagcttctt ctatctcctt cacctcgtac
      961 tcaaggaatg acagctcacg gcttctttct tcattgtcga tatttgactt ttcatactca
     1021 tccttaagcg ccctatatgc tttatatgtg actgaaagct gctgcttttt atctccaagc
     1081 ggctgctttg cgtactcatc aagtatatca aggtgttttt tctttgataa aagtgactgg
     1141 tgctcatgct gaccgtggat atctatgaga tatgcggcta tttccttcat tcgcgaaaca
     1201 gacacggctt caccgtttac cctgccgact gcgcgcgact gtgtgatttt acggcttaaa
     1261 atcacgcatc cgtcctcgac ctccacatca agctccctta aagcctctat aatctttggg
     1321 tcctcaactg aaaaaataag ctccacaaaa gcaggctttt cattgtctct tagcatctcc
     1381 ttttgcactt tttcaccaag cgccagattg atagagccta tgatgattga ttttccggct
     1441 ccggtctctc ccgacagtat atttagtccg tcggaaaact caacctcaca ctcatctatg
     1501 agtgccagat tctttacatg tagattttgt aacatatttt ctccttcctc tcccaggcat
     1561 gacgatatat gttattttct gttacagttc agctataatc tgatattcat gtaacattat
     1621 tccacaattc tgcggatttt tttcatcacc gcatatgtat catcaacagt ccggattgca
     1681 cacatgattg tgtcatctcc tgcaatcgaa ccgacgattt ctctcatctt catggcatct
     1741 attgcagcac agacagcgct tgccattccc gatacagtct ttataacgag gatattctgt
     1801 gccatatcca ttgacaaaaa gccctctctg agtactcttt cgtatttctt tgagagattc
     1861 tcaggtgcat ccgttatcac ggcgtacttc tgtctgccgc ctgcggtagg aatctttgtg
     1921 agcttaagct ccctgatatc cctggatact gtggcctgag tcacgcgaaa accatctttt
     1981 tcaagaagcg cagacagctc atcctgcgtc tctacctcat tcttttggat tatctcaatt
     2041 atcttggatt gtctgtttgt tttcatgtcc tgccacctca taaatattat ttgtaactat
     2101 tcattataac aatccttatc tgtgttatga ataatttccc atctttttgc ggagtatctc
     2161 caaaaagctt ctctggtgaa gcttgcaaat ctttgtatga ttggctgcct ttgaaataac
     2221 aaaacggtct cccacagcca gctctgccgt agtgtcgcca tcatagctga cacacgccat
     2281 ctcatcgttt tgctccctgc ggcttagaat ctctatctct atctcatcat cgcctgaaag
     2341 cacaatgctc tttgacgtaa gattgtgagc attgttcggt gtgagcagta tcatatcgcc
     2401 cttcggatcc acaatcggac ctcccgccga catactgtag cctgtagagc cnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cctttctatg gatgactata tcatttaatg
     2581 caactctcga gatttcactg cctcttttat gccctgtgag cataatacga tcctctgtca
     2641 tgtatttatc agccatgagc gaatcaatag cgtcaaatac cgtagcttcc tccacctcac
     2701 acagatagcc gagtgtgccg agattcactc ccataagcgg aatctcaagt gtctccaccc
     2761 ttgtggctgc acgaatcagt gtaccatctc cgccgagtac aagaatacac tgcgtatcct
     2821 gtggtatatc ctcaagctca aactcgttat cggatatagc ctcgacattg ctcataagcc
     2881 cctttgccgt gccgcccttt tgttctatgt atgccactat tttatttgta agtgcaaaat
     2941 tcctgtcctt ataggcattt gctatcacaa caaaatgttt catagtctat ttcctgtcca
     3001 gctctccatg cgcggcctcg accacactat gaatatccac tgattcctcc tttataggat
     3061 catctgtctt tttgatatgt acaaggtact ctatattgcc ctccggcccc ttgattggcg
     3121 aatactcaag gtctaacacg ctaaatccat ttgaaagtgc aaaggatatg atattgttca
     3181 ctacctcctc atgcacgctc ttgtcacgga ccacgccctt tttacctacc ttttccctgc
     3241 ctgcctcaaa ctgtggcttg ataagacaaa ccatctcgcc accgtccttt aacagtgctc
     3301 ttgcaggacc taacaccttg gtaagtgata tgaatgacac atcaaccgat gcaaaatcca
     3361 gctcatcatc tatgtcattg ggcgtcacat acctgatatt ggtcttttcc atgcacacca
     3421 cgcgctcgtc ctgacgcagc ttccatgcaa actggccata tccgacatcc acggcataca
     3481 cctttttagc accattttga agcatacagt ctgtaaaacc gcctgttgaa gcacctatat
     3541 ccatgcacac cttgccatcc atcgtgatat caaagtgctt catagccttt tcaagcttta
     3601 gccctccacg gctcacatac tgcagtgtct ttccatgaat ctcaatattg cagtcatcag
     3661 gaaatgtgct tcccgcctta tcctcacggt tgttatttac aaaaacattg ccctccataa
     3721 tcatggtctt tgctttttcc cgtgatggtg caaggcctct gtttacaagt aatacgtcta
     3781 atctttcttt catactgtat gttacctcta tctataatgc tgctattata cgcttagcca
     3841 ccgattcggc atccacatgc agctcctttt tgagcacctc gacattgcca tgctccacat
     3901 agcagtcagg aagtgcaact gtcaacacct caagagctat gccgttagtc ttgacatact
     3961 ccgtcacatg ctcaccaaaa ccgccgcttt gcacgttttc ctccatagtc acaagcaggc
     4021 tgtgttcaga cggcagttcc ttaattgctt tcttatcaaa aggcattgca aaacgcgcat
     4081 tgataagtgt agcagagatt ccctctgcct caagcagctt tactacatca acggcagtct
     4141 ttaccatact gccgagtgcc atgatagcca ccgtgcttcc ttttctgatt agctcactct
     4201 ttgcaagctc tatcggctgt ctgatttcct taaggccgtc ataggctgcc cctcttggat
     4261 atctgagtgc aatcggaccg tcatatgcca ctgcaaactt catcatatca gacagctccc
     4321 acttattctt cggagccatg attgtcatat tcgggatgac cgacagatat gatatgtcaa
     4381 atattccctg atgtgtctct ccgtcactgc ctacaagccc tgccctgtcg attgcaaaga
     4441 tgacatgcag attctgtatg cacacatcgt gaagtatctg gtcaaacgcc ctttgtaaaa
     4501 atgacgagta caccgcaaat accgggataa gtcctgcctt tgcaagccct gccgcaaagg
     4561 tcgtggcatg tgcctcggct attcccacat caaaaaatct atccgggaaa ttgcggtgga
     4621 atctgcgaag ccctgttcca tccgccatgg cagccgttat cgcaactacc tttgggtttc
     4681 tctcgcccag cttcttcatg actgttgaga aaacatctgt gtaattggcc ttcgtgcgct
     4741 tattctttgg aagccctgtc tctataacaa acggctctgc accgtgaaat ctagaaggat
     4801 aacgctcagc cgggccatag cctgctcctt ttttcgtgag cacatgcaca agcaccggac
     4861 catccacacg ctttgcctca tcaaacacat ggcacatttc ctttatatca ctgccatcca
     4921 caggtcccaa atagatgatg cccatctcct caaagaacat acccggaata aaaagctgct
     4981 ttatgctgct ctttgtattc ctgatatgct ttaccatacg ctctccgtag attggtatat
     5041 ggtttaggga attcataaca ttattcttta aatccctgta tgcatccgct gtgcgaaagc
     5101 ctgaaagata ctggctgatg cccccgacgt tctctgaaat cgacatattg ttgtcgttta
     5161 acactataat aaaattgcct ttaacgcttg aagcgttgtt gagcgcctca tatgccatgc
     5221 ctccggtcag cgcaccgtct cctattacag aaaccacgtg gtagtcttct cctgttacct
     5281 gtctggctgt cgcaagccca agtcccgctg agatggatgt ggagctgtgt cctgtattaa
     5341 agcagtcaca gtcgctctca tccgcctttg ggaagccact catgcctcca tactgtctga
     5401 gcgatgaaaa tccatccttt ctgccggtga gtatcttgtg tgtgtacgac tgatgcccta
     5461 catcccacac tatcttatcc ttcggcagat taaagcacag atgcagcgcc atggtaagct
     5521 ccacaactcc gaggtttgat gccagatgac ctcctgtcac cgatatgctc tctataagaa
     5581 actgcctgat ttcatccttt aacagctcaa gctcctcagg tgtcagtttt tttatatcat
     5641 tttccttttg tatcttttct aaaaccatct tatttctccc gatggatcaa atattcaaat
     5701 agctccttaa gaaatgcatc gtcgtatccc aaatcatcaa gctgctcctc tgcctccttg
     5761 gagatgcgct ccacatcaga aaccgcttta tccaatccct caaatgtgac atatgtcgct
     5821 ttattatttt tctcgtcact gcctaccggc tttccaagca cctcaagtgt acttgtgaca
     5881 tcaagcacat catcctgtat ctgaaacgca agccccagct ttgccgctat ctgctcaacg
     5941 cgtgatacct catcagacga cgcccctgca aggattgctc ctatcatcat cgagctctct
     6001 ataagtgcgc ctgtcttaag cctgtagata aagtcaagct tttcccttgt gatagaacag
     6061 tcatcggatt tctcgctttc cacatcaacc acctgtcctc cgaccatgcc atacacgcct
     6121 gctttttttg caagcaccgc aaatgccctt gccacacgcg tatcagccac ttccatgtca
     6181 aaagcctttg ctgcggtctc atatgccagg tttaaaagtg catcacctgc aagaatgccc
     6241 atagcctcac cgtacacagc atgtgtggtc ttctttccac gcctgtactc atcattgtcc
     6301 atggcaggca ggtcatcatg caccagcgag taggtgtgta tcatctcgat tgccgccata
     6361 aatggctcaa tcactgcact gcttccgcca aacatatggt aagtctcttc catgaggata
     6421 gggcgaagcc tctttccacc ggctctcaca ctatagttca tggcctcaaa aataatcctc
     6481 tgctgtccct cctcagcggg aagaaacttc tcaagcacat cgtttatatg ctctaccttt
     6541 tgctgcagct cttccatgaa ttgtgactta tttaaattcc catttaagct catcagaatt
     6601 cctccagttg tccgtcgctg tttagcacaa gcatggcctt ttccatgccc tgtaccatat
     6661 cattggcact ctttacctca tcaagcccct tcttgtagag ctcaaaggcc tcatccaggg
     6721 atacatccgc atcttccatc ctcgccacta tctgctctaa gtgtgcaaag cgctcctcga
     6781 gtgtcagtgt tttttcattt tctttgctca tcgttaataa cctccgaaac tacggccctt
     6841 gcatgaccgt ctatcagata tatatttatg tcatcgcctg cattgatatc ggatacactg
     6901 tgcagtgccc ccttcttttc cacctcgaca tacgcatagc cctgtgccag ctttttggcc
     6961 ggtgaatatg cgtccagtgt acctgaaagc agtgccagac gctgtctgca atccgaaagc
     7021 ctctgcttca taatcctttc aagtgcatcc tcgatatctg ccagacgcct tttattctca
     7081 tttagtttat tctgtggact aaggaaatta agtcttgttt taatatgtga cagcttttcc
     7141 ctgaaaaaat caatccggcc tgtcagattt ctgtccatcc tgcggcgcag attgtcaagg
     7201 cgctctaacg tctgtctgta gtcactgact gcaagctctg cagccgctga aggtgtgggc
     7261 gctctcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnngg cagtcaaata tggctctcgc cacaatctcc tcattaaagg
     7381 cccataaatc ctctatggag cctccgcctc tgccgactat tatcacatca agattcatcg
     7441 catccaatgt ctgtatgccc ttcactatgc tatcagccgc accctctccc tgcacaagtg
     7501 ccggataaag tatgacctgt acatatggat tcctgcgcga tgatacattc cttatatcct
     7561 gcacggccgc gcctgtcggt gctgtaacca caccaatacg gccggcatac
//
LOCUS       DPUF01000049           17758 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_969, whole
            genome shotgun sequence.
ACCESSION   DPUF01000049 DPUF01000000
VERSION     DPUF01000049.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 17758)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17758)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17758
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_969"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="DHV81_01730"
     CDS             <1..205
                     /locus_tag="DHV81_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93251.1"
                     /translation="AEADAAMIKKYVDAIKGEDRVPEKEYEARLAVCKACDRLNAGTC
                     NACGCYVELRALTGVSHCPHKKW"
     gene            complement(263..1198)
                     /locus_tag="DHV81_01735"
     CDS             complement(263..1198)
                     /locus_tag="DHV81_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI93252.1"
                     /translation="MIHITSLDDGLELFKALGSDIRIQILKILLENNQMSMNQIANEL
                     NISNGALTGHIKKLEECGLISASNDSSGHGNQKLCSLIQDRILVEIEKPIDLSNVYNT
                     SIKVGQFSSHNICPTCGLATSSFVIGELDDARYFDHPDHFNADIMWFTKGYVEYVIPN
                     LIPRNQKITQLSLSAEISSEAPGIDNDWPSDISFYINDTLVGTWTSPGDYGDVRGMFT
                     PEWWPQNWNQYGLLKLLVINHKGTFIDGLKISDITTTELKLDYTSTIRFRIAVEEDSA
                     HVGGLTIFGKSFGNYDQDIIVSINYEPLDTEKNTK"
     gene            1536..3098
                     /locus_tag="DHV81_01740"
     CDS             1536..3098
                     /locus_tag="DHV81_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCI93253.1"
                     /translation="MAEQDKCIRDKGENMAKIIVNNENKKSTIAPEIYGHFSEHLGRC
                     IYEGLFVGENSDIPNVNGMRIDVVDALKEMKIPVLRWPGGCFADEYHWMDGIGPKEKR
                     KKMINTHWGGVVEDNSFGTHEFFELCRQLGCKTYVNGNLGSGTVREMSEWVEYITFNG
                     VSPMADLRKENGHEDPWTIDYFGVGNENWGCGGNMRPEHYADEYRRYQTYVRNYAGNQ
                     PINKICCGPNVDDYEWTKKVMATCFDHCDPKLHGLMDGLSLHYYTLPETEDDWNIKGS
                     ATEFTEDIFYQTLKRGYFMEELINRHGAIMDEYDPDKNIGLIVDEWGIWSDVEPGTNP
                     GFLYQQNTMRDALVAGMTLNIFNKHSDRVKMACIAQLINVLQSVMLTDGDKMIKTPTY
                     YVFHMMRHHQGAALLDSSLVGGATVGSGKNELPKVFESVSEDKDGVITVTLTNNSLES
                     SEDVDIMLTNEGDKYSVSEARYIEGAMDAHNTFEAPEVVDEKDFTAYENTQTGVKVTL
                     PACSVVTLRLSK"
     gene            3266..5191
                     /locus_tag="DHV81_01745"
     CDS             3266..5191
                     /locus_tag="DHV81_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93254.1"
                     /translation="MDLKNIKIEDPFWGHMQELVTDVVIPFQEDVLNDRQPGVEKSHA
                     IENFKIAAGLSQGEFYGMVFQDSDVAKWLEGVAYSLIIKPDAALEKRADDIIDIIAAA
                     QQPDGYLNTYFTIKEPEHRWQNLLECHELYCAGHMMEAAVAYYEATGKDKLLKVMEGM
                     AGHIIDRFGPDKLPGIPGHQEVEIGLMRMYHATGNEAYKKQARYFLEERGKNPAFFAE
                     EAAKRDWNHFGMVPKDIKYNQSHATIYEQEEAVGHSVRAVYMYTAMADLAATEHDEKL
                     FAACERLWNNMTEKKMYITGGIGSTVEGEAFTKEYELPNDMAYAETCASIGLVFFAKQ
                     MLKMNKNGKYADAMERELYNGIISGMQLDGKRFFYVNPLEVNPGVSGEIFGYKHVIPE
                     RPGWYACACCPPNLVRMVTSLGRYAWDEDDDVIYSHLFIGQEARLKKADIKVVSEYPW
                     KGHVSYSITPKTGDEFAVAIHIPGYLKSFEVTLNGMRLKENSEAKADVFYSYRDGYIY
                     IKNKWHDNDVIEISFNMDIRVIYANTKVREDIGCAALQRGPVVYAFEGVDNDDDVQSL
                     MIDVSRLNEAQIETEAEGILKGAVLLDIPAVRLEGSDALYSEKPPRQKSVIARAIPYY
                     MWGNRGLNQMRVWMHTI"
     gene            5926..7227
                     /locus_tag="DHV81_01750"
     CDS             5926..7227
                     /locus_tag="DHV81_01750"
                     /inference="COORDINATES: protein motif:HMM:PF13416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93255.1"
                     /translation="MIKLKKIMALGLSALMVTSLVGCGGTTNATENKKSDSSKGTEIT
                     FWNSFTGADGDMLVKMVDKFNKENKDGIKVKMDISSDFDSQLSTAFAAGEGPTMILSS
                     SAYRFTYGDYLQDVSDVFDKTDLDKTDFIQSYLDYCSDDDKTYFVPFQVVGYYMYWNK
                     DLFKQAGLDPETPPANWDEWAKDAAKITDSSKNIYGSGISYDYAYQIAHIMQRFGGLA
                     VTDDNGKWKANFENNAGYEKFLNMYKDMIDKGDNPVEADTDSMVSAGQVGITVGGPWV
                     TAGLDTAGVDYGIGLIPQGDAGDMNSVEVIGFGITTAASDAEKEAAYKFIEWWNTQDK
                     DGSSPALEWSLKNGFPAYTYSVQNNDEYKANKKLSAMSAANPEAPTDFIVDSNFPGIN
                     AVLSDVIPEMINSVAFGNSSVEEALTKAQEATQKIVDKYNK"
     gene            7305..8189
                     /locus_tag="DHV81_01755"
     CDS             7305..8189
                     /locus_tag="DHV81_01755"
                     /inference="COORDINATES: protein motif:HMM:PF00528.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI93256.1"
                     /translation="MEKIKRYWNKPSRSAYLFIAPSIVLLILFSIIPLIMAFGLSFFD
                     VPITMNHATFIGWDNFVEAFHDPRFINSLKVTAIFTGIEVPVQVLGALIIAAFISKNN
                     KRNKFLRAVYFLPVICSATVIGIMWRMILHSNIGFITAVLQSMGLGKINFMNTPGLTI
                     FVISFISIWRSFGISAIIYVTAIQQVSPSLYEAAQMDGAGKILQFVNITVPSIRPTFW
                     YIMMTRFAGALQIFDIIYVTTNGGPNYTTESTVSYIYSRAFSSNSSMGYASAMSVVLF
                     IIIMFITVLMYKRMVKED"
     gene            8194..9039
                     /locus_tag="DHV81_01760"
     CDS             8194..9039
                     /locus_tag="DHV81_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI93257.1"
                     /translation="MKKKKTIGIGRFVFNIPVALILIIIAMMVLVPVVWVTLSAFKTE
                     KEIISWPPTFIPKTFTLENFVDVQGRIDIIRYIMNSVIYAGATTALAVIVNSMAGYAY
                     AFYDFKGKTASFLMTLATMMVPFQVIMVPLFLVVYKMGMYDTYWGLIIPRVAVAGSIF
                     MMRAAFTGIPKEMAEAARIDGLTEFGIFWKIMLPQVKPAVITLVILSVNGSWNDLLWP
                     MIVTSSTEMRTLANGLALFIGQNTIEYGAAFAGALVSLLPMFILYMFGQKYFVEGMSS
                     GGLKG"
     gene            9052..10563
                     /locus_tag="DHV81_01765"
     CDS             9052..10563
                     /locus_tag="DHV81_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCI93258.1"
                     /translation="MEITKIICNKNFQIDKVDSRLFGSFLEHMGRVIYSGIYEPDNIN
                     SDEDGFRKDVLEQVKQMNVTTIRYPGGNFVSAYHWEDGVGPKEKRPHKIELAWKSIET
                     NEFGTNEFMKWAKKANINPIFTVNLGTRGVEDAAHYLEYCNFPGGTEYSDLRKMHGVD
                     KPYGIKLWCLGNEMDGSWQVGHKSAEEYGKLAAETGKVMKIIDPEIELIVCGSSLSSM
                     ETYPEWDMEVLDKAYDIADYLALHQYYAGQEKGTPGFLAQSLDMEEYIHTIRSAAQVI
                     KQKKKSKKDMKFSVDEWGVWAVPSNTVNNELNEEPWQIAPAISEQIYTLEDALLFAEM
                     QMVILRNADAIKIACQSLLTNISACIMTEKGGNHWLQTIYYPFYYFANYAKGIVMKTM
                     SDGPAYSCKEFPKVSYIDSLSVWNKEENELVFFAVNRDEFSKQVISLQLSDFVSEKVI
                     ESISMTADDKKMTNQYNHEAVKPKTVNNAKINNQEVKIELEPLSFNMVRIKIK"
     gene            10682..11686
                     /locus_tag="DHV81_01770"
     CDS             10682..11686
                     /locus_tag="DHV81_01770"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00356.19,HMM:PF00532.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HCI93259.1"
                     /translation="MGDMYMAITVNQIAEKCGVSRTTVLRALNGGSVSKETKERILQV
                     AKDNNYRPNLLARSLNHGRTMSLGVVTINIENMYFVQSLNTINKEADKRGYFTNIVVC
                     DDSLEWEKKLIQGLAERQMEGILINPINKGESFEQFLLSLHVPVVCIGNQVSGKITTV
                     QVNEMVATMDAVKHIVSKDYEKIVFVCPPLEMKETQNTYTHEQRELGFRAAMGMYPDV
                     EMNVIGRENFLQDVQSVCKDGLNKKTAFLCSGDSYALMIMQWAAKEGYEIPKDFGLMG
                     FDDISVLQYISPKLTTVSTNVEGVAATAVVELIQQIESEDYSPKSIYLNHKVLDRDTL
                     "
     gene            11730..12695
                     /locus_tag="DHV81_01775"
     CDS             11730..12695
                     /locus_tag="DHV81_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCI93260.1"
                     /translation="MNKNNFVETSYNKPFIKQRADPYIYKHIDGSYYFTASVPEYDRI
                     VLRHSDTMIGLKDAEEVTIWRKHESGIMSVHIWAPELHYLDNKWFIYYAAGDIDDIWA
                     IRPYVLECEGQDPVNDSWKELGLVKRNDDFSFNDFSLDMTVFGNKGKRYCVWAEKVNI
                     GKKISNLYIAQMKSATELATPQVLLTSPDFEWERVGFWVNEGPAYLEHNGKIFLTYSA
                     SETGECYCMGMLSIDENKNLLNPHEWKKEYKPILNTDIDKQIYGPGHNSFVKDENGND
                     IMIYHARQYNEIIGDPLYDHNRHAYRMKIIWDIEGNPVFDYENNF"
     gene            12726..14819
                     /locus_tag="DHV81_01780"
     CDS             12726..14819
                     /locus_tag="DHV81_01780"
                     /inference="COORDINATES: protein motif:HMM:PF16990.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 31"
                     /protein_id="HCI93261.1"
                     /translation="MQKLFKRIISTLTAIVVICACVLPVQAKIDGNGALVYKSDNSST
                     FDPGGATYSRIICLKNSGSSNGTLLCTFDQLKKVTVMVNGNKVSKQVYPIYQSTDSGN
                     SWKLISNVYDKEYGLLRTSQPCLYELPKQVGDMPAGTILLAGNIFDDDPYKQSRIVIY
                     KSLDSGKTWSFLSEVDNGGPCTYDPSVTSTTTTVWEPFLNLSKDGRLVCYYSDERQKA
                     NGVLQAVSFKTSSDGKNWSSLSNVAAITNKKDRPGMITVSSLPNGKYIATYEVVNRPS
                     ISKNNAIVYCKFSDDGVTWDEGSLGTRVALSNGRGIGSSPYVKWVDAGGPNGMVIISA
                     KWATDSSDNINGGQNFYVNYNLGKGAWERLPMAVTYDASDTSGGYFSGFSQSFDVSPD
                     NGVLYQATNVENFGNKKTIKGVSYNLNDVRVGTLPLNGTIYEAEKAKLSNVKAESAVD
                     ASNGQEVRYINETDSIVDFENIKVSKSGNYTILVRYSNGETIEASHTVTVNGTTIGTI
                     NYPVTVNWDRYQWSKFTCYLKKGVNSIRFTKNVGFAEMDCIMIDNTDSDFVIENENSG
                     KYLEIKSALITENADAGQWGVTNNWCQKWTFEDAGNGYYRIKNMNSGLYLEIENQSKE
                     NGAKAVQCKYSNRASQLWKFVETGDGYYFIINKNSGKYLEVANNSTADGASVGQWEST
                     GYKCQKWTLRKEGIQ"
     gene            14977..16473
                     /locus_tag="DHV81_01785"
     CDS             14977..16473
                     /locus_tag="DHV81_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCI93262.1"
                     /translation="MLAVKNYKFWFCTGSQDLYGDECLANVAAHSKEIVAKLNESGKL
                     PFEVVWKPTLITNELIRKTFNEANTDDECAGVIVWCHTFSPAKSWILGLKELRKPLLH
                     LHTQYNEEIPYDSIDMDFMNENQAAHGDREWGHIVTRMGIERKVVVGHWSDPVVQEKI
                     ASWQRVAVGVVESSHIRVMRVADNMRNVAVTEGDKVEAQIKFGWEVDAYPVNEIAESV
                     AAVSQSDTNALVDEYYDKYDILLEGRDPEEFKKHVAVQAQIELGFERFLEEKNYQAIV
                     THFGDLGALKQLPGLAIQRLMEKGYGFGAEGDWKVAAMVRLMKIMTAGKKDAKGTSML
                     EDYTYNLMKGKEGILEAHMLEICPSIADGPISIKCQPLTMGDREDPARLVFTSKEGTG
                     IATSLVDLGDRFRLIINTVDCKKTEKPMPKLPVATNFWTPQPDLYTGAEAWILAGGAH
                     HTAFTYDLTAEQMGEWAAMMGIEAVFIDNDTTIRNFKKDLMLGNIFYK"
     gene            complement(16623..>17758)
                     /locus_tag="DHV81_01790"
     CDS             complement(16623..>17758)
                     /locus_tag="DHV81_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCI93263.1"
                     /translation="MRGLKDEPFMLMEQTPSQQNWQHYNSLKRPGQMRAQSYQTIAHG
                     ADTIQFFQLRRSKGGCEKFHGAVIAHVGTNDTRVFRETAQLGRELESFGTRTLGTRNK
                     SDVGIIFDWDNYWALEYTSGPTQDLKYVDQIHHYYEYFYNKNISVDMIPVDGDFSKYK
                     VIAAPVLYMVKEGMKEKLEQFVKNGGTLITTFMSGIVDQSDNVHLGGYPGPLREMAGV
                     WVEEIDALAPEQSNTVSFSDGKEYKCNLLCDLMHPEGAEVLATYESDFYAGMPAVTKN
                     SYGKGHVYYVATQLEAAGLARVLDEATNEVSVSGIIAEETSLEITCRESENTRFYFVM
                     NFTDEKQTLPASLAGMVDMITGEAAKEGDVMNKWDVKILQEAL"
BASE COUNT         5808 a         2668 c         4102 g         5180 t
ORIGIN      
        1 ggcagaggcc gacgcagcaa tgataaaaaa atatgttgat gcgataaagg gcgaggacag
       61 agtgccggaa aaagagtatg aggcaaggct tgcagtgtgc aaggcgtgtg acaggctaaa
      121 tgccggaacc tgtaatgcat gcggctgtta tgtggagctt cgggcgctta caggcgtttc
      181 gcactgccct cataaaaagt ggtagtacaa ataaaataat aacaaaatga ctgcggtttt
      241 gatttccgca gtcattttat tattatttag tgtttttttc agtgtcaaga ggttcataat
      301 tgatggatac aataatgtcc tgatcgtagt taccgaagct ttttccgaat attgtgagtc
      361 cgccgacatg ggcagaatcc tcctcaactg cgatacggaa gcgtatggtg cttgtgtagt
      421 caagctttag ctcagtggtt gtgatatctg atatcttgag accatctatg aaggtgcctt
      481 tatgatttat gactaaaagc ttgagtagac cgtattgatt ccagttctga ggccaccatt
      541 caggggtaaa cattccgcgt acatctccat agtcaccggg agaggtccag gtgccgacaa
      601 gagtgtcatt tatgtaaaag cttatatcac ttggccagtc gttgtcgatg cccggagcct
      661 cagaacttat ctcggctgag agtgaaagct gggtgatttt ctgattacgt ggaatgaggt
      721 ttggaattac atattccaca tagccttttg tgaaccacat aatgtctgca ttgaaatggt
      781 caggatggtc aaagtaacgc gcgtcatcga gctctcctat cacaaatgag gatgtagcca
      841 ggccacaggt tggacagata ttgtgagatg aaaactgccc tacttttatg gatgtgttgt
      901 atacgttact taagtctatt ggtttttcaa tttctactaa tatacgatcc tgaatcagtg
      961 aacacagctt ctggttgccg tgaccggatg aatcgtttga ggcggagata agtccacact
     1021 cctcaagctt ttttatatgt ccggttaaag ctccattgct gatgtttagc tcgtttgcaa
     1081 tctgattcat gctcatctgg ttattctcga gcagaatttt taaaatctgg atacgaatat
     1141 cagagccgag agctttaaac agttcgagac catcgtctaa tgatgtgata tgtatcatat
     1201 atagattctc ctaaaatatt ttataaaatt aacaacgagt tcttgtgtgt aaatcatgtt
     1261 ttaattatag gaataagctc ctatgttgtc aatatattat tgtatagttt taagctatat
     1321 ggaaataggt tttaaaatta tttaaatata ttgtgcaata tgtataactt acatgcgttt
     1381 caaaacgaaa ataaaataaa attttagata ttattaaaca atatgtaaaa tagtttaaaa
     1441 taatctaaaa taaatattga cattgcagct actctcatgt atattatgtg cataagcaaa
     1501 acacattaac aatcagtgca cttatgtgtg caacaatggc agagcaggat aaatgtataa
     1561 gagataaagg agaaaatatg gcaaaaatta ttgtaaataa tgagaataaa aagagtacaa
     1621 ttgctccgga gatttatgga catttttcag agcatctcgg aagatgtatc tacgagggat
     1681 tatttgtagg ggagaattca gatataccaa acgtaaacgg tatgcgtatt gatgttgtag
     1741 atgcattaaa ggaaatgaag atacctgtgc tccgctggcc gggcggctgc tttgcagatg
     1801 agtatcattg gatggatggt atcggtccta aggagaagag aaaaaagatg atcaacacac
     1861 actggggtgg tgtagttgag gacaacagct tcggaacaca tgagtttttt gagctttgca
     1921 gacagttagg ctgtaagaca tatgtgaacg gaaatttagg aagcggaaca gtgcgcgaga
     1981 tgagtgagtg ggtagagtac atcacattta acggagtatc acctatggct gatttgcgta
     2041 aggaaaacgg acacgaagac ccatggacta ttgactactt cggtgtggga aatgagaact
     2101 ggggatgtgg tggaaacatg cgtcctgagc attatgcaga tgaataccgc agataccaga
     2161 cttatgtcag aaattatgca ggcaatcagc caatcaataa aatctgctgt ggtccaaatg
     2221 tggatgacta tgagtggaca aagaaggtta tggctacatg ctttgaccat tgtgatccga
     2281 aactccatgg tctgatggat ggattatctc ttcattatta cacacttcct gagactgagg
     2341 atgactggaa tatcaaggga agtgcaacag agtttacaga ggatatcttt tatcagactc
     2401 ttaagagagg atatttcatg gaggagctca tcaatcgtca tggagccata atggatgagt
     2461 atgatcctga taagaatata ggtcttattg ttgatgagtg gggcatctgg tcagatgtgg
     2521 agcctggtac caatccggga ttcttatatc agcagaacac aatgcgtgat gcgcttgttg
     2581 caggtatgac attaaacatt ttcaacaagc attccgaccg tgtcaagatg gcatgtattg
     2641 cacagcttat aaatgtgctt cagtctgtaa tgctcacaga cggcgataag atgataaaga
     2701 cacctacata ttatgtattc catatgatga gacatcatca gggcgcagct cttttggaca
     2761 gctctcttgt aggaggagct actgttggtt ctggaaagaa tgagcttcct aaggtttttg
     2821 agtctgtatc agaggataag gatggagtga ttacagtcac acttacaaac aattctcttg
     2881 agtcgtcaga ggatgttgat attatgctta caaatgaggg tgacaaatac agtgtaagtg
     2941 aggccagata catcgaaggt gccatggatg cacacaatac ctttgaagca cctgaggtag
     3001 tggatgagaa ggatttcaca gcatatgaaa atacacagac cggtgtgaag gttactcttc
     3061 cggcatgcag tgttgttaca cttagattat cgaaataata ttaaaacagc tattcagaat
     3121 cacgtggaat aaaaagcagg ttttcctgtt cctcaagtgg ttctgaatag ttatgtatta
     3181 aaagaaaaat cgctttttat gatgcttgca aatattggct ggtcaatttt gctggttgaa
     3241 gtattttata tggagagagt aggatatgga tttaaagaat ataaaaatag aggatccgtt
     3301 ttggggacat atgcaggagc ttgtgacaga tgttgtcatt ccgtttcagg aagatgtgct
     3361 taatgacaga cagcccggag tggaaaaaag tcatgcaata gagaatttta agattgcagc
     3421 aggcctttca cagggcgagt tttacggtat ggtctttcag gacagtgacg tggcaaaatg
     3481 gcttgaggga gtagcatatt ctctgataat aaagcctgat gctgcgcttg aaaagagagc
     3541 tgatgacatt atagatatta tcgctgcagc acagcagccg gatggatatc tgaatacata
     3601 tttcacaata aaagagcctg agcacagatg gcagaatctg cttgagtgtc acgagctgta
     3661 ctgtgcaggt catatgatgg aggctgctgt cgcatattat gaggctaccg gcaaggataa
     3721 gctgcttaag gttatggaag gcatggcggg acacatcata gacagattcg ggcctgataa
     3781 gctgcccgga ataccggggc atcaggaggt tgaaataggt ctgatgcgca tgtatcatgc
     3841 aacaggaaat gaggcctaca aaaagcaggc gagatatttt cttgaggaga ggggaaaaaa
     3901 tcctgctttt tttgctgagg aagccgcaaa gcgtgattgg aatcactttg gaatggttcc
     3961 taaggatata aagtataacc agagccatgc taccatttat gaacaggaag aagcggtagg
     4021 acacagtgtg cgtgctgtct atatgtatac agccatggca gatctggcag caacggagca
     4081 cgatgaaaag ctttttgctg cctgtgagcg actgtggaat aacatgacag aaaagaagat
     4141 gtatataaca ggaggtatag gctcaacagt ggagggagag gcctttacga aggagtatga
     4201 gctgccgaat gatatggcat acgctgagac ctgtgcatcg ataggtcttg ttttctttgc
     4261 aaagcagatg cttaaaatga acaaaaacgg caaatatgca gatgccatgg agagggaatt
     4321 atacaacgga attataagtg gaatgcagct tgatggaaag agattttttt atgtgaatcc
     4381 gcttgaggta aatccaggag tatcaggtga aatatttggc tataagcatg tcattcctga
     4441 aagaccgggc tggtatgcat gtgcatgctg tcctccgaat cttgtgcgta tggttacatc
     4501 tctgggcaga tatgcgtggg atgaggatga tgatgtaatc tattcacatc ttttcatcgg
     4561 tcaggaggct cggcttaaaa aagcagatat aaaggttgtc agtgaatatc cgtggaaggg
     4621 acatgtttca tacagcatta cgccgaagac aggcgatgaa tttgccgttg caatacatat
     4681 tccgggatat ctgaagagct ttgaggtgac actcaatggc atgaggttaa aagagaatag
     4741 tgaggcaaaa gcagatgtgt tctattctta tagggatggc tatatttata taaaaaataa
     4801 atggcatgat aacgatgtta tagaaatcag cttcaatatg gatatccgtg taatatatgc
     4861 caatacaaag gtaagagagg acataggatg tgcagctttg caaagaggtc cggtagtgta
     4921 cgcttttgaa ggagtggaca atgatgatga tgtgcagagt ctgatgatag atgtgtcaag
     4981 gcttaatgaa gcacagattg aaacagaagc tgagggaata ttaaagggtg cggttctttt
     5041 agatattcct gcagtgagac ttgaaggctc agatgcgcta tattctgaga aaccacctag
     5101 acaaaagtct gtaatagcaa gggctatacc atattatatg tggggaaatc gaggacttaa
     5161 tcagatgagg gtatggatgc atactattta aataaaattg cagagtatgt tacagtagtt
     5221 tcacagttgg atacaaacgc actcgtagat gggtactatg acaaatacga cattctgctt
     5281 gagcgtagaa atttttagaa gttgaaaagc atgtagcggt acaggtacag attgggctcg
     5341 gatttgagaa atcccttgag gaaaagaatt atcaggtaat cgttacacct tttggcaatc
     5401 tttgagtatt aaagaaactt ctgggcacca cagcctgact tttatacagg tgcagaggca
     5461 tgaatgcttg caggaggagc acatcataca gtgttcacat atgaccttac agctgagcag
     5521 atggatgagt gagttaccat gatgggtata gaggctgtat ttgtcgacaa tgacacaaca
     5581 atcagaaaat tcaagaaggg acttatgctt gaaaatattt tctacaaata aagttaataa
     5641 ttactaaaaa aaggacagcc gagatgtggt tgtctttttt ttataactta atatgtacac
     5701 gagtacaaat ggatataaat gaaaaattat gaaaaataaa caaaaaaacc gctaaaataa
     5761 taacaaaaac aacaaaaatg taaatgcata ttgacaatac aagatggcgg agttatatta
     5821 tggatataag acaacgtaca cgtgtacaaa acaagcaaat agaggaagta tggagaagat
     5881 acctgacgag gtagattgct ccaggtattc cggaatggag actatatgat taagttaaag
     5941 aaaattatgg cattggggtt gtcagcgttg atggttacat cacttgttgg atgtggagga
     6001 acaaccaatg caacagaaaa caaaaagtca gactcatcaa aaggaacaga gattactttt
     6061 tggaatagct ttacaggagc agacggggat atgcttgtaa agatggttga caagtttaat
     6121 aaagaaaaca aggatggaat aaaagttaaa atggatattt catcggattt tgatagtcag
     6181 ctttctacag catttgctgc aggtgaggga ccgacaatga tactaagctc aagtgcctat
     6241 cgttttacat atggggatta tttacaagat gtaagtgatg ttttcgataa aacagatctt
     6301 gataagacgg attttatcca atcatatttg gattattgtt cagatgatga caaaacgtat
     6361 tttgtaccat ttcaggtagt gggttattat atgtactgga ataaagattt atttaagcag
     6421 gcagggcttg atcctgaaac acctcctgca aattgggatg aatgggcaaa ggatgctgct
     6481 aagataactg attctagtaa gaatatatat ggctcaggta tttcatacga ttatgcatat
     6541 cagattgcac atattatgca gaggtttggc ggactcgctg taacagatga caatggcaag
     6601 tggaaagcta attttgaaaa taatgcaggc tatgagaaat tccttaatat gtataaagat
     6661 atgatcgata agggggataa tccggtagag gctgatactg attcgatggt aagtgcgggt
     6721 caggtcggaa taacagttgg tggtccatgg gttacagcag gtctggatac agctggtgtt
     6781 gattatggta ttggtcttat tccacaaggc gatgcaggtg atatgaattc agttgaagta
     6841 atcggatttg gaattacaac agctgcaagt gatgctgaaa aggaagctgc ttacaagttt
     6901 attgagtggt ggaatacaca ggacaaagat ggaagtagtc cggcattaga gtggtcactt
     6961 aagaatggtt tcccagcata tacatattca gtgcagaata atgatgagta taaagctaat
     7021 aagaaactta gtgctatgtc agccgcaaat cctgaagcac caacagattt catagttgat
     7081 tcaaatttcc ctggaattaa tgcagtatta tcagatgtta ttccggaaat gattaattca
     7141 gttgcatttg gtaattcatc agttgaggag gcattaacaa aggcacagga agcaactcag
     7201 aaaattgttg ataagtataa caaatagcgg taattgtcaa attatagtac aagtaagggt
     7261 ggcttaaata gtcaccctta cgaatagaaa ggatggaata agtgatggaa aaaatcaaaa
     7321 ggtattggaa taagccatcc agaagtgcat atctgtttat agctccatca atagtattat
     7381 tgatattgtt cagtataatt cctttaatta tggcgtttgg attaagtttt tttgatgtac
     7441 cgattacaat gaatcatgca acatttatag gatgggataa ttttgttgaa gcatttcatg
     7501 atccgcgatt tataaacagt ttgaaggtga cagcaatttt tactggaata gaagttcctg
     7561 ttcaagtgtt gggtgctttg ataattgcgg cgtttataag taaaaataat aaaagaaata
     7621 agttcttacg tgcagtgtat ttcctcccag taatttgttc tgcaactgta attggcatta
     7681 tgtggagaat gattcttcat tcaaatattg gatttataac agctgtatta cagtccatgg
     7741 gattaggaaa aattaacttt atgaatactc cgggacttac aatttttgta atctcgttca
     7801 tatcaatatg gagaagtttt ggtatttcag caataattta tgttacagca attcagcaag
     7861 tatcaccatc gttgtatgaa gcagcacaaa tggatggtgc aggaaagata cttcaatttg
     7921 ttaatataac agttccttct ataaggccta cattttggta tattatgatg acgagatttg
     7981 ctggagcgct tcagattttt gatattatat atgtgacaac aaatggtgga ccaaattata
     8041 caacagaatc aacagttagt tatatatatt ccagagcatt ctcatcaaac tcaagtatgg
     8101 gatatgcatc ggcaatgtcg gtggtacttt tcattatcat catgttcatt accgttttga
     8161 tgtataaaag aatggtaaag gaggactgat gatatgaaaa agaaaaaaac aataggcatt
     8221 ggcaggtttg tttttaacat accagtcgct ttaattctaa taattatagc tatgatggtt
     8281 ttggtgccag ttgtttgggt gacattaagt gcatttaaaa cggagaaaga aattatatca
     8341 tggccaccta catttattcc gaaaacattt acattggaaa attttgttga tgtacaagga
     8401 agaatagata ttattcgtta catcatgaat tctgtaattt atgcaggtgc aacaacagcg
     8461 ttagcggtta ttgtgaatag tatggcggga tatgcatatg cattttatga ttttaagggc
     8521 aaaacagctt cgtttcttat gacacttgca acaatgatgg ttccatttca ggttattatg
     8581 gttccgttat tcttggttgt atataaaatg ggaatgtatg atacatattg gggtcttata
     8641 attccacgag tagctgttgc aggatcaatt tttatgatga gagctgcgtt tacaggaatt
     8701 ccaaaagaga tggcagaagc tgcaagaata gacggtttaa cagaatttgg tattttttgg
     8761 aagataatgc ttccacaggt gaagccagct gtaattacac ttgttattct aagcgtaaat
     8821 ggatcttgga atgacttgtt atggcctatg attgttacaa gtagtacaga aatgcgtact
     8881 cttgcaaatg gtttggcatt gtttatcgga cagaatacaa tagagtatgg agctgcattt
     8941 gcaggagctc tagtctcatt acttccaatg tttattctat atatgtttgg acaaaagtat
     9001 tttgttgaag gcatgagtag tggcggatta aaaggatagg agttgaataa tatggaaatt
     9061 acaaagatta tatgtaataa aaatttccaa attgataagg tggattctcg gttatttggc
     9121 tcttttctcg agcacatggg aagagttatt tattcaggaa tatatgagcc ggataatatt
     9181 aattctgatg aagacggatt tagaaaagat gtgttagaac aagtaaaaca aatgaatgta
     9241 actacaattc gttatccggg aggaaatttt gtttctgctt atcactggga ggatggagtt
     9301 ggacctaaag aaaaaagacc tcataagata gaattagcct ggaaatccat tgagacaaat
     9361 gaatttggaa caaatgaatt tatgaaatgg gcaaagaaag ctaatattaa tcctattttt
     9421 acagtgaatt taggaactcg tggagttgag gatgcagcac attatttgga atattgtaat
     9481 tttccgggtg gtacagagta tagcgattta cgtaaaatgc acggagtaga caaaccttat
     9541 ggtataaagc tttggtgtct tggaaatgag atggatggaa gctggcaggt gggacataag
     9601 tcagcggaag aatatggaaa acttgcggca gagacaggaa aagtgatgaa gataatagat
     9661 ccggaaattg aattaattgt ttgcggaagt tcattgtcaa gcatggaaac ttatccggaa
     9721 tgggatatgg aagtgcttga taaggcatat gatattgcag attatcttgc gttacatcag
     9781 tattatgcag gccaggaaaa gggaacaccg ggattccttg ctcaatcgct tgacatggaa
     9841 gaatatatcc atactattag atccgcagct caggtcataa aacagaaaaa gaagtcaaaa
     9901 aaagatatga aattcagtgt tgatgagtgg ggagtatggg ctgtacctag caatactgtt
     9961 aataatgaat taaatgaaga accatggcag atagcccctg caataagtga gcagatatat
    10021 actcttgaag atgctttgct ttttgcagag atgcagatgg ttattcttag gaatgcagat
    10081 gcaattaaaa ttgcatgcca gtctttactt actaacataa gtgcttgcat aatgactgaa
    10141 aagggaggaa atcactggtt gcagacaata tactatcctt tttattattt tgcaaactat
    10201 gcaaaaggta ttgtgatgaa aactatgagt gatggtcccg cttacagctg taaagagttt
    10261 ccaaaagtat catatataga tagtctgagt gtttggaata aagaagaaaa tgaattagtt
    10321 ttttttgcag ttaatcgtga tgaatttagt aaacaggtga tttctttaca attgtcagat
    10381 tttgtgtcag aaaaagtgat tgaaagcata agtatgacag cggatgataa aaagatgaca
    10441 aatcagtata atcatgaggc agtcaaacct aaaacagtca ataatgcaaa aattaataat
    10501 caggaggtga aaatagaatt agagccatta tcatttaata tggtcagaat aaaaataaaa
    10561 taatactata aattatggaa tgatatagag aaaagagaca actcattgta gaaaccatga
    10621 tattcatgat ataatcatat tagttttaag ttaatgtata tatgagttta taatattttg
    10681 tatgggggat atgtatatgg caattacagt aaatcagata gcagaaaagt gtggtgtttc
    10741 cagaactacg gtacttagag cattaaatgg cggaagtgta tcaaaggaaa caaaagaacg
    10801 catactgcag gtcgcaaagg ataataatta ccgaccaaat ttacttgcta gaagtcttaa
    10861 tcatggaaga actatgtcgc ttggtgttgt aactattaat attgaaaata tgtattttgt
    10921 acagtctttg aacacaatta ataaagaggc agataaaaga gggtatttta ctaatatagt
    10981 agtttgtgac gatagccttg aatgggagaa aaaacttatt caaggtcttg ccgagagaca
    11041 gatggaggga atattgataa acccaataaa taaaggcgag agctttgaac aatttctctt
    11101 atcattacat gttccagtgg tgtgtattgg caatcaggta tcaggaaaaa taacaactgt
    11161 tcaggtaaat gaaatggttg caaccatgga tgcggtaaaa catattgtat caaaagacta
    11221 tgaaaaaata gtatttgttt gtccaccttt agagatgaaa gagacacaaa atacttacac
    11281 acatgagcag agggaattag ggtttagagc tgctatggga atgtacccgg atgtagaaat
    11341 gaatgtgata ggcagagaaa attttttaca agatgttcag tctgtttgta aggatggttt
    11401 aaataaaaag acagctttct tatgttctgg agatagttat gctcttatga taatgcaatg
    11461 ggctgctaaa gaaggctatg agattccaaa agattttgga ttgatgggat tcgatgatat
    11521 aagtgtttta caatatattt ctccaaagct tacaacggta tctacgaatg ttgaaggagt
    11581 tgctgctact gcggtagttg aacttataca acagatagaa tctgaggatt attcaccgaa
    11641 gtctatatat ttaaatcaca aagttttgga tagagataca ttgtagtaac tgcattcata
    11701 tttcctgaat tttcaaggag attcaaaata tgaataagaa taattttgtt gaaacaagtt
    11761 ataacaaacc gtttattaaa caacgggcag atccatatat ttataaacat atagatggaa
    11821 gttattattt tacagcctcc gtaccagagt atgacagaat agtgttacgt catagtgata
    11881 caatgatcgg actgaaggat gcagaggaag taactatatg gagaaaacat gaaagcggaa
    11941 taatgagtgt acatatatgg gctccggaac ttcattattt ggataataaa tggtttattt
    12001 attatgcagc aggggacatt gatgatatat gggctataag accatatgtt ttggagtgtg
    12061 aagggcaaga tcctgttaat gattcgtgga aagaattagg tttagttaag aggaatgatg
    12121 atttttcttt taatgatttt tctttagata tgactgtgtt tggaaataaa ggtaagaggt
    12181 attgtgtgtg ggctgagaaa gtaaatattg gaaaaaagat atctaatcta tatattgcac
    12241 agatgaaatc agccacagaa ttagctacac cacaagtttt gttaacatca cctgattttg
    12301 aatgggagag agtaggattt tgggtgaatg aaggaccggc atatcttgag cataatggta
    12361 aaatttttct gacatattca gcgagtgaaa caggagaatg ttattgcatg ggtatgctaa
    12421 gtattgatga aaataaaaat ctattgaatc cacatgaatg gaagaaggaa tataagccaa
    12481 ttctaaatac tgatattgat aaacagatat atggtccagg acataattca tttgttaagg
    12541 atgaaaatgg taatgatata atgatttatc atgcaagaca gtataatgaa ataattggag
    12601 atcctcttta tgatcataat aggcatgctt atcgaatgaa gataatttgg gatatagaag
    12661 gtaatccggt ttttgattat gagaataatt tttagaacgg tattgttttt gggaggaaaa
    12721 tatatatgca aaaactattt aaaagaataa tttcaacatt aacagcaatt gttgttattt
    12781 gtgcgtgcgt tttgcctgta caggcaaaaa ttgatggaaa tggtgcactt gtttataaat
    12841 cggataatag ttctactttt gatccgggag gggctacata ttctagaatt atatgcttaa
    12901 aaaacagtgg aagttcaaat ggaactttat tatgtacatt tgatcagtta aaaaaagtta
    12961 cggtaatggt taatggtaac aaagtttcaa aacaagttta tccaatttat caaagcacag
    13021 acagtggaaa cagttggaaa ttaatttcta atgtatatga taaagaatat ggacttttac
    13081 gtacatcaca gccatgcctg tatgaacttc caaagcaagt cggtgatatg ccggcaggaa
    13141 ctattttgtt agcaggtaat atatttgatg atgatcctta taaacagtca agaattgtta
    13201 tttataagag tttggacagt ggaaagacat ggtctttttt aagtgaagtt gataatggag
    13261 gaccttgtac atatgatcct agtgttacat ctactactac tacagtatgg gaaccattcc
    13321 tgaatctgag caaggatgga aggttggttt gttattattc ggatgagaga cagaaagcaa
    13381 atggtgtcct tcaagcagtt tcgtttaaaa catcgtctga tggtaagaat tggagttcat
    13441 tatcgaatgt ggcagcaata acaaacaaaa aagacagacc aggtatgatt acagttagtt
    13501 cgctgccaaa tggtaaatat attgcaacat atgaagttgt taatagacca agtattagta
    13561 agaataatgc tatagtatac tgtaagtttt cggatgatgg ggttacatgg gacgaaggta
    13621 gcttaggaac aagagttgca cttagtaacg gacgaggtat tggttcatcc ccttatgtaa
    13681 aatgggtaga tgctggtgga cccaatggta tggttattat atctgcaaaa tgggcaactg
    13741 attcaagtga taatataaat ggtggacaaa atttttatgt gaattataat ctgggtaagg
    13801 gtgcatggga gagattacct atggctgtta cttatgacgc tagcgataca tcaggcggat
    13861 atttttcagg atttagtcag agctttgatg tgagtcctga taatggtgtt ttatatcaag
    13921 caacaaatgt tgaaaatttt ggtaataaga aaacaattaa aggtgtgagc tataatttaa
    13981 atgatgtaag agttggtaca cttccactta atggaacaat ttatgaagct gaaaaagcaa
    14041 agttatcaaa tgttaaggct gagagtgcag ttgatgcttc aaatggtcag gaggttcgtt
    14101 atattaacga aacagacagt attgttgatt ttgaaaatat aaaagtatca aaaagtggaa
    14161 attatactat tttagtaaga tattccaatg gcgagacaat tgaagcctct catactgtga
    14221 cagtcaacgg aacaacaatt ggtactataa attatccggt tacagtaaat tgggatagat
    14281 atcaatggtc aaagtttaca tgttatttaa aaaaaggtgt caattcaatc aggtttacaa
    14341 aaaatgtggg ttttgcagag atggattgta taatgattga taatactgat agtgattttg
    14401 ttattgaaaa tgaaaatagt ggaaagtatc ttgaaattaa aagcgcattg ataacggaaa
    14461 atgcggatgc aggacaatgg ggggttacaa ataattggtg tcagaaatgg acatttgaag
    14521 atgcaggaaa tggctattat agaattaaaa atatgaattc tggtttatat cttgaaatag
    14581 aaaatcagag taaagaaaat ggagcaaaag ctgtacaatg taaatatagt aatagggcat
    14641 cgcaattatg gaagtttgtt gaaactggtg atggttacta ttttataatt aataagaata
    14701 gtggaaaata tttggaggtt gcgaataatt ctacagctga tggagcttct gttggacaat
    14761 gggaatcgac tggatataag tgtcagaaat ggactttgag aaaagaaggt attcaataat
    14821 aacaaaatag ttaaaatact gctattaaaa accaaaaaat aaacataaaa gcttgttcta
    14881 atatataatg atttgagtta ttatatattt gcaatggaaa acatttataa aaacaagcaa
    14941 acgtaaataa aaacaacaaa ataaaggaga ggaactatgt tagcagtaaa aaattacaaa
    15001 ttctggttct gcaccggctc gcaggacctg tacggagatg agtgccttgc aaatgttgca
    15061 gcacattcaa aggagattgt agccaaatta aatgagtcag gaaagcttcc ttttgaggtg
    15121 gtttggaagc cgacactcat cacaaatgag ctcatcagaa agaccttcaa cgaggccaat
    15181 acagatgatg agtgcgccgg tgttatcgta tggtgccata cattttcacc tgcaaagtca
    15241 tggattttag gactcaagga gctcagaaag ccgttacttc atttacatac acagtacaat
    15301 gaggagattc catacgattc gattgatatg gacttcatga atgagaatca ggcagctcac
    15361 ggtgacagag agtggggaca cattgttaca cgtatgggta tcgagagaaa ggtcgttgtg
    15421 ggacactggt cagatcctgt ggtacaggaa aagattgcat catggcagcg tgtagcagtc
    15481 ggtgtggtag agagcagtca catccgtgta atgcgtgtag ctgacaatat gagaaatgtt
    15541 gctgttacag agggtgacaa ggtagaggct cagattaagt ttggctggga agtggatgct
    15601 taccctgtaa atgaaattgc agagtctgtt gcagcagttt cacagtcaga tacaaacgca
    15661 ctcgtagatg agtactatga caaatacgat atcctgcttg agggcagaga tcctgaggag
    15721 ttcaagaagc atgtagcggt acaggcacag attgagcttg gatttgagag attccttgag
    15781 gagaagaatt atcaggcaat cgtaacacac tttggtgatc ttggagcatt aaagcagctt
    15841 ccgggacttg caatccagcg tctcatggaa aagggctatg gctttggagc tgagggagac
    15901 tggaaggttg cagcaatggt tcgtctcatg aagatcatga cagcaggaaa gaaggatgcc
    15961 aagggtacat caatgctcga ggactacacc tacaatctca tgaagggcaa agagggaata
    16021 ctcgaggctc atatgcttga gatttgtcca tcaatcgcag acggtcctat aagcatcaag
    16081 tgccagccac ttacaatggg tgacagagag gatccggcaa gacttgtatt tacatcaaag
    16141 gagggcaccg gtatcgccac atcacttgtt gatttgggtg accgtttccg tctcattatc
    16201 aatacagttg actgcaagaa gacagagaag cctatgccaa agcttcctgt agcaacaaac
    16261 ttctggacac cacagcctga cctttacaca ggtgcagagg catggatact tgcaggagga
    16321 gcacatcata cagcgttcac ttatgacctt actgctgagc agatgggcga gtgggcagcc
    16381 atgatgggta ttgaggctgt atttatcgac aatgatacaa caatcagaaa cttcaagaag
    16441 gatcttatgc ttggaaatat tttttacaaa taaaatatag ctatgcttaa taattaaaat
    16501 cccgggttgt ctgtcacact aaggtgtgta cggacaaccc gggattttgc cggttgtgaa
    16561 tgatacaaaa atgcagcagc tttttgtgta gcttactata tgttttaatg cataaggccc
    16621 tcttatagtg cttcctgaag tatcttcaca tcccacttat tcatcacatc gccctcttta
    16681 gctgcttctc cggtgatcat gtcaaccatt cctgcaaggc ttgcagggag agtctgcttt
    16741 tcatcagtga aattcatcac aaagtaaaag cgtgtatttt cgctctcgcg gcatgtgatt
    16801 tcaagactcg tctcttcagc aatgatacct gatacagaca cttcatttgt ggcttcatca
    16861 agcacccttg caagtccggc tgcctcaagc tgtgtggcaa catagtatac atggcctttt
    16921 ccgtagctgt tttttgtgac agcaggcatt cctgcataga aatcgctttc atatgtggcg
    16981 agtacttctg caccttccgg gtgcatcagg tcgcacagca gattgcattt atattctttt
    17041 ccatccgaaa agcttacagt gtttgactgc tccggtgcga gcgcgtcaat ttcctcaacc
    17101 cagacacctg ccatctcgcg aagcggtccc ggatatccgc ccagatgcac gttgtcagac
    17161 tggtccacaa taccactcat aaatgttgtg atgagagtac cgccgttttt cacaaactgc
    17221 tcaagctttt ccttcatgcc ttcctttacc atgtacagaa caggtgcggc aatcacctta
    17281 tattttgaaa aatcaccgtc aaccggaatc atatcgactg agatattttt gttgtaaaaa
    17341 tactcataat agtggtgaat ctggtcaaca tatttaaggt cctgtgtagg tccgcttgtg
    17401 tactcaagtg cccagtaatt gtcccagtca aagatgattc ccacatctga tttattacgt
    17461 gtgccaagtg tccttgtgcc gaagctttca agctcccttc caagctgtgc ggtttctctg
    17521 aatactcttg tatcgtttgt tccgacatgt gctattacag caccgtggaa tttctcgcag
    17581 ccacccttgc ttcgtctgag ctggaagaac tggatagtgt ctgcaccgtg ggctatggtc
    17641 tgatagctct gtgcacgcat ctgtcccgga cgcttcaatg aattgtagtg ctgccagttc
    17701 tgctggcttg gagtctgctc catgagcatg aatggctcat ccttaagacc tcgcatca
//
LOCUS       DPUF01000050            3783 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1204, whole
            genome shotgun sequence.
ACCESSION   DPUF01000050 DPUF01000000
VERSION     DPUF01000050.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1204"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>146)
                     /locus_tag="DHV81_01795"
     CDS             complement(<1..>146)
                     /locus_tag="DHV81_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCI93264.1"
                     /translation="SRYSIVIAAAKRARQIIDGAEPLIARPKCNKPLSIAVEELYTGA
                     VRIVS"
     assembly_gap    147..241
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<242..828)
                     /locus_tag="DHV81_01800"
     CDS             complement(<242..828)
                     /locus_tag="DHV81_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCI93265.1"
                     /translation="MSNTGKLVVFSGFSGSGKGTIMKELMAKHGDDYALSVSATTRGP
                     RPGEEHGREYFFISEEEFEQMIKADGLLEYAKYVDHYYGTPKSYVNEQLSAGKNVILE
                     IEIQGALKIKKQFPDTVLMFVSAPSADELRDRLVGRGTETKEVCAQRLSRAYEESLGI
                     EKYDYLVVNDKLDDCVELVNDIIHSSDDTKKNQDYL"
     gene            complement(844..1863)
                     /locus_tag="DHV81_01805"
     CDS             complement(844..1863)
                     /locus_tag="DHV81_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCI93266.1"
                     /translation="MMFLLISYKYSKLLNIYNHEYLYYNIFDENLKELYNKLIYAYAK
                     GKIMIKSMTGFGRYEYADASRKITVEVKSVNHRYLDVNIKMPKKLNFFESAIRTLLKE
                     YIERGKVDIYITYEDFTENNLSLQYNKALAGEYLKYLNQMAEEFGLENDIRVSTLSRY
                     PEVFAMEEQPVDEDEMWSSLEKALRGAFEPFVESRVREGENLKKDLCEKLDNMVSYVD
                     FIEERSPQIIVEYRARLEEKLRELLADSQLDDSRIAQEVTIFADKICVDEETVRLKSH
                     ILSMKDSLNAGGSVGRKLDFLAQEMNREANTILSKSNDLKISDTGISLKTDIEKVREQ
                     IQNIE"
     gene            1862..3623
                     /locus_tag="DHV81_01810"
                     /pseudo
     CDS             1862..3623
                     /locus_tag="DHV81_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742513.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    3301..3341
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1089 a          885 c          672 g         1001 t
ORIGIN      
        1 gagacgattc tgacagctcc cgtatacagc tcctctacag caatagataa tggcttattg
       61 cacttagggc gtgcgataag tggttctgca ccatcaataa tctggcgtgc acgctttgct
      121 gcggcaataa cgatagaata acggctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nagataatcc tgattctttt ttgtgtcgtc agagctgtgg atgatatcat tcacaagctc
      301 tacacaatcg tcaagcttat catttaccac taaataatca tatttctcta tgcctaaaga
      361 ctcctcatat gctcttgaaa gtctctgtgc acagacctct tttgtctctg tgcctcttcc
      421 tacaagccgg tccctaagct catctgcact cggcgctgac acaaacataa gcaccgtatc
      481 aggaaactgc ttctttatct ttaaagctcc ctggatttct atctcaagaa tcacattctt
      541 tccggcgcta agctgctcat tgacatatga ctttggtgtg ccgtagtaat gatctacata
      601 cttggcatac tcaagcagtc catctgcctt tatcatctgt tcaaattctt cctctgagat
      661 aaagaaatac tctctgccat gctcctcacc cggccttgga cctctggtcg tggctgatac
      721 gctcagtgca taatcatcac catgctttgc cataagctcc ttcatgatag taccctttcc
      781 ggaacccgaa aagcctgaaa aaacaactaa ttttcctgtg ttactcatca caatctccta
      841 aaattactca atattttgaa tctgttccct gaccttttct atatcagttt taagactgat
      901 gcctgtgtca gaaatcttaa gatcattgga ctttgaaagg atggtattcg cctcacggtt
      961 catctcctgt gcgaggaaat caagctttct tcccacactg ccaccggcat ttaacgaatc
     1021 cttcatggaa aggatatggc ttttgagcct gactgtctcc tcatccacac agatcttgtc
     1081 cgcaaaaata gtgacctcct gggctattct tgaatcatca agctgactgt ctgcaagcag
     1141 ctctcttagc ttttcctcaa gacgcgcacg gtattcgaca ataatctgtg gcgaacgctc
     1201 ctcaatgaaa tcaacatatg ataccatatt atcgagcttt tcgcaaaggt cttttttaag
     1261 attttctccc tcgcgcacgc ggctttcaac aaatggctca aatgcaccgc gaagagcctt
     1321 ctcaagactg ctccacatct catcctcatc tacaggctgt tcctccatcg caaatacctc
     1381 cggatatctc gacagcgtgc tcactcttat atcattctcc agtccgaatt cctccgccat
     1441 ctgattcaga tacttaagat attctcccgc gagagcctta ttatactgca atgagagatt
     1501 attttctgta aaatcctcat atgtgatata aatatcaacc ttgccacgct ctatgtactc
     1561 cttaagcaga gtccttattg cactctcgaa gaaattgagc tttttaggca tcttgatatt
     1621 gacatccaga tatctgtgat ttacagattt tacctcaact gtaatcttac ggcttgcatc
     1681 tgcgtactcg tatctgccaa agccggtcat acttttaatc ataatttttc ctttcgcata
     1741 tgcatagatg agtttattat ataactcctt gagattttcg tcaaatatat tataatataa
     1801 atattcatga ttataaatat tcagcaactt agaatattta taggaaataa ggaggaacat
     1861 catggcttta gacggaatag tcatttcaaa tatagttgca gagctaaaca gcacgatact
     1921 aaactcaaaa atcagtaaaa tcgcagaacc tgaggcagat gagctgcttc ttaccctaaa
     1981 gggaccaaac ggctcgttca ggctttcgat gtctgcaagt gcatctcttc cattcatcta
     2041 cctgacaccc accaataagg taagccccct cactgcccct acattttgca tggttttaag
     2101 aaagcatata gcaaacggac gcatcacaaa aatttaccag ccgggcatgg agcgtatcat
     2161 aaactttgaa atcgagcact taaacgagat gggcgatcta tgccacaagg tgcttattat
     2221 cgagctgatg ggcaaatatt caaacatcat ctttaccgac tccgatggta cgataataga
     2281 cagtgccaag cgtattcccg catccgtaag ctctgtgcgt gaggtgcttc cgggcagagc
     2341 ctacactatc ccagcgacac aggaggataa atataatcct ctgactgtag attcgaagca
     2401 gtttgttgat attatatcag caaagccgct tactgtttca aaggctatat actcatcctt
     2461 ttccggcatc agcccccttg tggcaaatga gctggctcac agggcaggcc ttgacgcgga
     2521 ctcccctgta gctgcctact cacacgatga gcttttgcac ctgggaagca acttcacatg
     2581 gatgatggag gacataaaaa ataatcgttt tacgccgaat atcgtgcgtg acggcaatga
     2641 accaaaggaa ttttcttcaa tagagctgac tcagtatagc gatttaacgg tcacaaaata
     2701 tgaatccata tcagaggttt tagagcttta ctactctgag agaaacacct atacacgtat
     2761 tcgccagaag tcagccgacc tgagaaaaca cgtaaatacc ctcctggagc gaaaccagaa
     2821 gaaatattcc ctgcagatga agcagcttaa agactctgaa aaacgcgaaa agtacaaggt
     2881 atacggcgag cttatcaacg cattcggcta cggacttaca ccggacgaca agtttcttga
     2941 agctgcaaac tattatgatg acaacaaaat catcaaaatc cctatcgaca acacaaaaac
     3001 tcctgccgag aacgcgcaga aatactttga caaatacgga aagatgaaac gtactgccga
     3061 agcattgaac gagctgattc ttgaaacaaa aggccagata gaccatctcg aatcaattca
     3121 gaattcactg gatatcgctt taagcgctga cgacctcgtc cagataaagg atgagctgat
     3181 agaatatggt tttatcaaaa aaggaaaggg ttcaaagaaa cagaaggtaa agagcaaacc
     3241 cttccactat atctcatcag acggctttga tatgtacgtt ggcaaaaaca actaccagaa
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcaaccgga aacgactggt
     3361 ggttccatgc caagggcatg cccggctcac atgtcattgt aaaagctgaa aacaaagagc
     3421 tgccggacag cacctttgag gaggccggca agctcgcagg ctactactca aagggcaaaa
     3481 attccgacaa ggtagagata gactatctgc aaaagaaaaa tgtcaaaaag ccaaacggtg
     3541 ccgcagccgg ctttgtcgtg tattacacaa actattctct cacgattcac ccggatatat
     3601 ccgatatcag gcagattgag taagcagatt atacttattt cataacaatt tcataaccat
     3661 tccaaaaccc ttaaagtaaa aagctgatat acacatgcat agtcctgcat gcgatatcag
     3721 cttttatttt agccatttaa gctcatcaca gattcatcct ctttaattgt tcttttgcaa
     3781 att
//
LOCUS       DPUF01000051           10639 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3740, whole
            genome shotgun sequence.
ACCESSION   DPUF01000051 DPUF01000000
VERSION     DPUF01000051.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3740"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="DHV81_01815"
     CDS             complement(<1..1073)
                     /locus_tag="DHV81_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HCI93267.1"
                     /translation="MENNSLHKLGIDIGSTTVKIALLDADDNILFSDYERHFANIQET
                     LESLIRKAYDKTGDLSVCPMITGSGGLTLAKHLEVPFVQEVIAVSTALTHYAPQTDVA
                     IELGGEDAKIIYFEGGNVEQRMNGICAGGTGSFIDQMASLIQTDATGLNEYAKSYKAI
                     YPIAARCGVFAKTDIQPLINEGATREDLSASIFQAVVNQTISGLACGKPIKGHVAFLG
                     GPLHFLSELKSAFIRTLNLDDEHTIVPENSHLFAAIGSALNYKDNSVTTLSSLIDRLS
                     NGIKMEFEVARMEPLFKDQADYDEFTTRHGNNHVKTGDLSTYKGNCYLGIDAGSTTTK
                     IALVSEDGDLLYSFYSNNNGSPLA"
     assembly_gap    1337..1407
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1428..2270
                     /locus_tag="DHV81_01820"
     CDS             1428..2270
                     /locus_tag="DHV81_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93268.1"
                     /translation="MNWLNKLEQKYGSKAIPNITRIFIAANLIGSVLLLTKGDVVLYY
                     LQFSASAILHGQVWRLISWIFVPSGSISFLQLLFIFCLWTLGDSMESYLGSFRMNVYF
                     FGGFLLYDIVGMLAFAVTHVSINLTMYYILIAMYLMLGLLMPEAEVRLYFVLPIKMKW
                     MVLVYFVMFAYDVYTYMSFGWQIGLAYGSEIVLALINLLVFIYGCKHRLSFRQNMHQK
                     RRKAQFKAQFSEPRPGSGISRHKCYICGRTELSNPELTFRYCSKCTGNKEYCQDHLFT
                     HTHV"
     gene            2357..4074
                     /locus_tag="DHV81_01825"
                     /pseudo
     CDS             2357..4074
                     /locus_tag="DHV81_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516288.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; frameshifted; too many
                     ambiguous residues; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
     assembly_gap    2819..2856
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4118..4975
                     /locus_tag="DHV81_01830"
     CDS             4118..4975
                     /locus_tag="DHV81_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HCI93269.1"
                     /translation="MDLKSGIDKFGLNPEDINDLYDEDKAAATGDAPVAAAVQTEDET
                     DFVFAKNITCPVCDKSFQTLTVRTSRVRFLGSDDDFRPVYKGIDTIKYGVTSCPHCGY
                     SAMNGDFVHVSGTQIRLLKEQVAAKFKPRSKSVPLLYSYDDAIDRYKLALFSAIVKRA
                     PYSEKAYICLKMSWLYRGLIEQLTADGISAESEELASAQKSEKYYYEQALDGMTRAVA
                     TEHFPICGMNQDTVDLLLAQMNHKLGHLDVASKLVARVLISKSASRHIKDKALDLKNE
                     IIKKIRDVK"
     gene            complement(5071..>6276)
                     /locus_tag="DHV81_01835"
     CDS             complement(5071..>6276)
                     /locus_tag="DHV81_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCI93270.1"
                     /translation="AYSGGLDTTAIIPWLKETYDYDVICCCINCGQGEELDGLDERAK
                     LSGASKLYIEDICDEFSEDCIVPCVQAGAVYEHKYLLGTAMARPGIAKKLVEIARKEG
                     AVAICHGATGKGNDQIRFELGIKALAPDIKVIAPWRQDNWKMDSREAEIEYCKAHGID
                     LPFGTDSSYSRDRNLWHISHEGLELEDPSQEPNYDHLLVLSVTPEYAPDEGEYVTMTF
                     EKGVPTSVNGKKMKVADIIRELNRLGGKHGIGIIDIVENRVVGMKSRGVYETPGGTIL
                     MEAHDQLEELCLDRATMEVKKEMGNKLAQVVYEGKWFTPLREAIQAFVESTQEYVTGE
                     VKFKLYKGNIIKAGTTSPYSLYSESLASFTTGDLYDHHDADGFITLFGLPLKVRAMKL
                     LELENKKNN"
     assembly_gap    6277..6443
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6562..7620
                     /locus_tag="DHV81_01840"
     CDS             6562..7620
                     /locus_tag="DHV81_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCI93271.1"
                     /translation="MIKAGIIGATGYAGAELVRILMNHKEVEIAWYGSRSYVDENYAD
                     IYGNMFQIVDAKCMDDNMEELAESVDVIFTATPQGFLAGVLTEDILSKVKIVDLSADF
                     RIKDVATYEKWYKIEHKSPQFIDEAVYGLCEINRDKVTKETRLVANPGCYTTCSILTA
                     YPLVKEGLIDTDTLIVDAKSGTSGAGRGAKTPNLFCEVNESMKAYGVASHRHTPEIEE
                     QLGYAAGKEILINFTPHLVPMNRGILATEYATLKKKPDGTLPTYDEIKAVYDKYYANE
                     KFVRVLKKGVCPETKWVEGSNYVDVNFVIDERTGRIIMMGALDNLVKGAAGQAVQNMN
                     LLFGFDEAEGLNMVPMFP"
     gene            7704..8168
                     /locus_tag="DHV81_01845"
     CDS             7704..8168
                     /locus_tag="DHV81_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCI93272.1"
                     /translation="MSNENKYEIRVMTIDDYDEVYSLWQTIHGFGIRSIDDSREGVLR
                     FLKRNPTTSVVAVEDGKIVGAILCGHDGRRGCLYHVCVDEHYRKCGIGKSMVVACMRA
                     LQAEHINKVSIIAFKVNELGNHFWKEEGWTFREDLNYYDFTLNEENITRFNK"
     gene            8180..9082
                     /gene="argB"
                     /locus_tag="DHV81_01850"
     CDS             8180..9082
                     /gene="argB"
                     /locus_tag="DHV81_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCI93273.1"
                     /translation="MADKNMEQVMEKAQVLIEALPYIQRFNRKIIVVKYGGSAMVDEQ
                     LKKQVIQDVTLLKLVGFKPIIVHGGGKEISKWVGKVGMEPVFVNGLRKTDADTMEIAE
                     MVLNKVNKSLVTLVEELGVQAVGVSGKDGGLLKVKKKYSNGEDIGFVGEITDVNPKIL
                     YDLLEKDFLPIVCPIGLDDEYNTYNINADDAACAIARAVSAEKLAFLTDIEGVYKDPS
                     DKNTLISELTVSDAKKLIGDGFIGGGMLPKLNNCIDAIDNGVSRVHILDGRIAHCLLL
                     EIFTNRGIGTAILGDSEEKFNNEQ"
     gene            9072..10289
                     /locus_tag="DHV81_01855"
     CDS             9072..10289
                     /locus_tag="DHV81_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCI93274.1"
                     /translation="MSNNMDKQSYIDEAEKELLHTYNRFSLVLDHGEGVHLYDTDGNA
                     YLDFAAGIAVCALGYSNEHHKEALKAQVDKLLHTSNLYYNAPVIEAADKVLNASKMDR
                     IFFTNSGTEAIEGAIKAAKKYAFTRDGHAGHEIIAMNHSFHGRSIGALSVTGNAHYQE
                     PFEPLMPGVKFADFNDLESVKAQVTDKTCAIIMETIQGEGGIYPAKKEFIEGVRALCD
                     EKDILLILDEIQCGMGRSGEMFAWQNYDVKPDIMTCAKALGCGVPVGAFLMTQRVADK
                     SLAPGDHGTTYGGNPFVGAAVSAVFDEFKRLDIVSHVKEVAPYLEKRLDELVAKFDCL
                     TARRGMGLMQGVECSLPVGKVSAEALNQGLIVITAGSNVLRFVPPLVIEKSDVDEMIE
                     KLETTLVILSKSL"
     gene            10347..>10639
                     /locus_tag="DHV81_01860"
     CDS             10347..>10639
                     /locus_tag="DHV81_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCI93275.1"
                     /translation="MKKIILLSAFILTIAFGLCGCKKDDATVHFASETEQTAVVSSDV
                     NRQTADSKEKKASSDGEQEQKAKDTADDTDESTEWCVYICGQVANPGVYRVKPG"
BASE COUNT         3008 a         1926 c         2551 g         2878 t
ORIGIN      
        1 gcgagagggc ttccgttgtt gttgctgtag aatgagtata aaaggtctcc gtcctcgctt
       61 acgagagcga ttttagtggt ggttgagcct gcatctattc cgagatagca gttgcccttg
      121 tatgtagaaa ggtcaccggt ctttacgtga ttgttgccgt gacgcgttgt aaattcatcg
      181 taatcagcct gatccttaaa aagaggctcc atacgtgcca cctcaaattc catcttaatg
      241 ccgtttgaga ggcggtcgat taaggatgag agtgtggtga cagagttgtc tttatagttt
      301 aatgcagagc ctattgctgc aaacagatga gagttctcag gaacgatagt gtgctcgtca
      361 tcgagattta aggttctgat aaatgctgat ttaagttctg acaaaaagtg cagaggaccg
      421 ccaagaaaag caacatgtcc cttgattggc ttaccgcagg cgaggccact gatggtctgg
      481 tttaccacgg cctgaaagat tgaagcggat aaatcctcgc gtgtggctcc ctcattgatg
      541 agaggctgga tatctgtttt ggcaaatact ccacagcggg ctgcgatagg gtagatggcc
      601 ttgtagcttt ttgcgtattc atttagtcct gtagcgtctg tctggatgag agaagccatc
      661 tggtcgataa atgagcctgt accgccggca catatgccgt tcatacgctg ttccacgtta
      721 ccgccctcga agtatatgat tttggcatcc tcgccaccta gctctattgc cacatcggtc
      781 tgtggagcat agtgtgtgag tgcggtagat acagctataa cctcctgtac gaatggcacc
      841 tcaaggtgct ttgcaagagt gagtccgccg gagcctgtta tcataggaca aactgaaagg
      901 tcacctgtct tgtcgtaggc ctttctgata agggactcaa gtgtctcctg tatatttgca
      961 aaatgacgct cataatcgga aaagaggata ttgtcatcgg cgtcaagaag tgcaatcttg
     1021 acggttgttg agccgatatc aatgcccaac ttatgtaagc tgttattttc catagtggtt
     1081 cctccataat gtattactta tatgttttta tatatctttc ttcttattaa aatatataat
     1141 tgttctatta tcaaaacaga gattattata cgacagatat cgacaaatta caatgactat
     1201 aattataaag aatttgtgaa taattcgcag tctcgttgac aaagtatgat ttaactatca
     1261 taataaattt tgtaataacc gctgcccttt gcactatagt gtaaaaggca gcaataatat
     1321 gacaggagga tgcagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnaac catgtctgct aaaatgtatg aactggctta
     1441 acaaactaga acaaaaatat ggaagtaaag cgattccgaa tatcactaga atatttatcg
     1501 ctgcgaatct gataggcagt gtgctgcttt tgacaaaggg agatgttgtc ctttattatc
     1561 tgcagttttc agcttctgct attttgcacg gacaggtatg gcgtcttata agctggattt
     1621 ttgtgccgag cggcagcata tcatttttac agcttttatt tatattctgt ctgtggacgc
     1681 ttggagacag catggagtca tatcttggct cttttaggat gaatgtgtac tttttcggag
     1741 gctttttgct gtatgatata gtaggaatgc ttgcatttgc agtgacacat gtatccatca
     1801 atctgactat gtactatata cttatagcta tgtatctgat gcttggtctt ttgatgccgg
     1861 aggcagaggt gaggctttac tttgtgcttc ctataaagat gaagtggatg gtgcttgttt
     1921 acttcgtgat gtttgcatat gatgtgtata cctatatgag ctttggatgg cagattggat
     1981 tggcatacgg ctcagaaatc gtacttgcac ttataaatct gttggtattc atttatggct
     2041 gcaagcacag gctctcattc agacagaata tgcaccagaa gaggagaaag gcacagttta
     2101 aggctcagtt ttccgaacct cgcccgggaa gtggcatctc acgtcacaag tgctatatat
     2161 gcggcagaac ggaattgtca aatcctgaat taacattcag atactgctcg aagtgcaccg
     2221 gcaacaagga atactgtcag gatcatctgt ttacacatac gcacgtataa tatacatgta
     2281 ctactttttc taattcattg acaaagtaaa ctgatgaatt tataatggac acagtattat
     2341 tttcggagga acatttatgg agaacgaggc aacagtttcg aaaaatttta ttgaacagat
     2401 tattgagaag gacttggctg agggtcattg caagactgta aagaccagat tcccaccgga
     2461 gccaaacggt tatttgcata tcggccatgc gaagtctatt cttttaaact atggactggc
     2521 aaagaagtat gacggacagt tcaatctcag gtttgatgat acaaacccta ccaaggagcg
     2581 tttggagttt gtagagtcta tcgaggccga tgttaagtgg cttggtgctg actgggctga
     2641 caggaagttt tttgcatcca attacttcga caagatgtat gagtgtgcag taggactgat
     2701 aaagaaggga aaggcatatg tatctgatct gacacctgat gagataagag agtatagagg
     2761 tacactcaca gaggccggca aggaggatcc atactcaaag agaagtgttg aggaaaacnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttt ttgagcgcat gaaaaacggt
     2881 gagtttgagg atggcacaaa ggtgcttcgt gcgagaattg acatgagctc ttcaaacatc
     2941 aatatgagag atccgattct ttaccgtgtg gctcatatga cacatcacaa tacaggtgat
     3001 aaatggtgtg tatatcctat gtatgatttt gctcatccta tagaggatgc tattgagggc
     3061 atcacacatt ctatatgtac tcttgagttt gaagaccaca gaccgctgta tgactgggtg
     3121 gttattgaat gtggatttaa gaattctcct gaggagtcgc caaagcagat tgagtttgca
     3181 aagctctatc tgactaatgt tgtaacagga aagagatata tcaagcgtct tgtggaggac
     3241 ggcatcgttg atggctggga tgacccaagg cttgtttcta tagcagcgct cagacgcagg
     3301 ggcttcacac cggagtctat ccagaatttt gttgatctgt gtggtatatc aaaggctaac
     3361 agctctgttg actacgctat gcttgagtat tgtatccgag aggatttaaa ggctaagcgc
     3421 cctcgtatga tggctgtgct tgatccggtc aaggtgatta tcgacaatta ccctgaggga
     3481 caggttgagt atcttgaagt ggacaataac gtggagaatc ctgagcttgg aaagagaaag
     3541 gttcctttca ccagagagct ttatgtggag agagatgact tcatggagga gccaccgaag
     3601 aagtatttcc gtatgttccc tggcaatgag gtgcgtttca tgaacgctta ttttgtgaag
     3661 tgcaatagct gtgtgaagga cgaaaacggc aatattacag agattcactg tacatatgac
     3721 ccggcatcaa agggtggcaa cagtcctgat ggcagaaagg tcaagggaac tatccactgg
     3781 gtttcagccg agtatggaaa gcaggtaact gtacgtcttt acgagaatat cgtaaatgag
     3841 gagcttggag tgtacaatga ggacggaagc ttaaatctta atcctaattc actcacagtg
     3901 cttgataact gtgtagtgga gccggagctt atgagtgcca aggcttatga cagcttccag
     3961 tttgtcagaa atggcttctt ctgtgcggat tgcaaggact caaaggatgg acagcctgta
     4021 tttaacagaa tcgtctcatt aaagagctca ttcaagcttc cgacagctaa atagtttttg
     4081 actaaaaata aacagaagag aagaatagag gtaaaatatg gatttaaagt caggtattga
     4141 caaattcggt ttaaatcctg aagacataaa tgatctgtat gatgaggata aagcagctgc
     4201 caccggggat gctccggtgg cagctgctgt tcagacagag gatgaaacgg attttgtttt
     4261 cgcaaagaat ataacatgtc cggtgtgtga taagagcttt cagacactga cggtaaggac
     4321 ctcaagggtt cgctttcttg gttctgatga cgatttcaga ccggtataca agggaattga
     4381 tacaataaag tatggtgtca catcgtgtcc gcattgtggt tatagtgcga tgaatggcga
     4441 ctttgtacat gtatcgggta cacagataag gctgcttaag gagcaggttg ctgccaaatt
     4501 taagccccgc agcaagtctg tgcctttgtt atattcgtat gatgatgcga tagacagata
     4561 taagcttgca cttttttcgg caattgttaa aagagcaccg tacagcgaaa aggcatatat
     4621 ttgccttaag atgtcatggc tttatcgtgg cttaatcgaa cagcttacgg ctgatggtat
     4681 ctcagcggaa agtgaagagc ttgcaagtgc acaaaagtca gaaaaatatt attatgagca
     4741 ggctcttgat ggcatgacca gagcggttgc gacagagcat ttcccgatat gtggtatgaa
     4801 tcaggatact gttgatttac ttttagctca gatgaatcat aagcttgggc atctggatgt
     4861 agcttcaaag cttgttgcaa gggtgcttat atcaaaaagt gcttctagac acattaagga
     4921 taaggcgctt gatttaaaga atgaaattat taaaaaaata agagatgtga aataattgct
     4981 gttattttac atctctttta ttaattataa caattaattg taaaaacaaa ttacatgctg
     5041 ataattatat caggattaaa taattaagct ttaattattt ttcttgttct caagctcaag
     5101 aagcttcatt gcacgcacct tgagtggcag accaaacagt gtgatgaagc cgtctgcatc
     5161 atggtgatca tataaatctc ctgtagtaaa gcttgcaaga gactcactgt agagtgaata
     5221 tggtgaagta gtgccggcct tgatgatatt gcctttatat aatttgaatt taacttctcc
     5281 ggttacatat tcctgtgttg actctacgaa agcctggatt gcttcacgaa gtggtgtgaa
     5341 ccattttcct tcgtatacta cctgagcgag cttgttgccc atttctttct tgacctccat
     5401 tgtggcacgg tcaaggcaga gctcctcgag ctggtcatgt gcctccatga ggattgttcc
     5461 acccggagtc tcatacacac cacgtgattt catacctact acacggttct caacgatatc
     5521 gatgatgccg atgccgtgct tgccgccgag acggttgagc tctctgatga tgtctgcaac
     5581 cttcatcttt tttccgttga cagatgtagg aacacccttc tcaaatgtca ttgtaacata
     5641 ctcaccctcg tcaggagcat actcaggtgt gacgcttaat acgagcagat ggtcatagtt
     5701 tggctcctgt gaaggatcct caagctcaag tccctcgtgg ctgatgtgcc agaggttacg
     5761 gtcacggctg tagcttgaat ctgtgccgaa cggaaggtcg attccgtgtg ccttacagta
     5821 ctcgatctca gcctcacgtg aatccatttt ccagttgtcc tgacgccatg gagcgattac
     5881 cttgatatca ggagcaagtg ctttgatacc gagctcgaaa cggatctggt cgtttccttt
     5941 accagttgca ccgtggcaga tggctacagc gccttctttt cttgcaatct caacgagctt
     6001 ctttgcgatg cccggtcttg ccattgctgt tccaaggagg tatttgtgct cataaactgc
     6061 gccggcctgt acacaaggta cgatgcagtc ctcactgaac tcatcacaga tatcctctat
     6121 gtaaagcttt gaagctccgg ataatttagc tctctcatcg agtccgtcaa gctcctctcc
     6181 ctgtccgcag ttgatgcagc agcagattac gtcatagtca taagtctctt taagccatgg
     6241 aatgatagcg gtagtatcaa gtccgcctga ataagcnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnaattttg tataattttt ctctttacgg attgaaatta
     6481 ctgtactata ttattatgta tgatgcgtca gacccatttt atatggttat gcagaaatac
     6541 aatatgaaaa gaggaaatac tatgataaag gctggtatta ttggagccac cggatatgcc
     6601 ggagcagagc tcgtgagaat tttgatgaat cataaagagg tagagattgc atggtatggc
     6661 tcacgaagct atgtggatga gaattatgcc gatatctatg gaaatatgtt tcagatagtt
     6721 gatgcaaagt gcatggatga caatatggag gagcttgcag agtcggttga tgtgattttc
     6781 actgcgaccc cacagggctt tttggcaggc gtgctcactg aggatatatt aagtaaggta
     6841 aagattgtcg atctgtcagc ggacttccgt atcaaggatg tagccacata tgagaaatgg
     6901 tacaagattg agcacaagtc gccgcagttt attgatgagg ctgtatatgg tctgtgtgag
     6961 ataaacaggg acaaggtcac aaaggagaca aggcttgttg caaatcctgg ctgctatacc
     7021 acatgctcca tccttacagc ttatccgctt gtgaaggagg gacttattga cacagataca
     7081 cttatcgtcg atgcaaagtc aggcacatca ggcgcaggac gtggtgcaaa gacacctaat
     7141 ctgttttgtg aagtaaatga gagcatgaag gcatatggcg ttgcctcaca cagacatacc
     7201 cctgagatag aggaacagct tggatatgcg gcaggaaaag aaattcttat caactttact
     7261 ccgcatcttg ttccgatgaa cagaggaatc ctcgctacag agtatgcgac acttaagaaa
     7321 aaacctgatg gaacacttcc tacatatgat gagattaagg cagtttacga taagtattat
     7381 gcaaatgaga agtttgtcag agtgctcaaa aagggtgtct gccctgagac aaagtgggtt
     7441 gagggcagca actatgtgga tgtaaacttt gtgattgatg agaggaccgg cagaattatt
     7501 atgatgggtg ctcttgacaa tctggtaaag ggtgctgcag gacaggcggt tcagaatatg
     7561 aatctgttat ttggcttcga tgaggcagag ggactcaata tggttcctat gttcccgtaa
     7621 taattaataa taggaaagta attatcagaa agatgcatta taatgctgat agttatataa
     7681 aaatataagg atgatttaat aaaatgagta atgagaataa atatgaaata agggttatga
     7741 cgatagatga ttatgacgag gtgtacagcc tctggcagac catccacggc tttggaatcc
     7801 gttctattga cgactcaaga gagggagtcc ttagatttct taagagaaat ccgactacga
     7861 gtgttgtagc ggtagaagat ggaaagatag ttggagcgat actctgcgga cacgatggca
     7921 gaagaggctg cctgtatcat gtatgcgtgg atgagcatta caggaagtgc ggcatcggga
     7981 agagtatggt ggttgcctgc atgagagcac ttcaggcaga gcatatcaac aaggtgtcaa
     8041 tcatagcctt caaggtgaat gagctgggaa accacttctg gaaggaagag ggatggacat
     8101 tcagagaaga cttaaactac tatgatttca cacttaacga agaaaatata acaagattta
     8161 acaaataagg agcagtacaa tggcagataa gaacatggaa caggtaatgg aaaaggcaca
     8221 ggtgctcata gaggcacttc cgtatataca gagatttaac agaaagatta tcgttgtaaa
     8281 gtacggcgga tctgcaatgg ttgacgaaca gcttaaaaag caggtaatcc aggatgtcac
     8341 acttctcaag ctggtcggct tcaagcctat catcgtacac ggcggaggca aggaaatcag
     8401 caaatgggta ggaaaggtcg gcatggagcc tgtattcgta aacggactca gaaagactga
     8461 tgccgataca atggagattg ccgagatggt attgaataag gtaaacaaat ctctcgttac
     8521 actcgttgag gagcttggtg tgcaggctgt cggagtgagt ggcaaggacg gtggtctctt
     8581 aaaggtaaag aaaaagtatt caaacggaga ggatataggc tttgtcggtg aaatcactga
     8641 tgtcaatcca aaaattcttt atgacctgct tgagaaggat tttcttccaa ttgtgtgtcc
     8701 aatcggtctt gatgatgaat acaatacata caatatcaat gcagatgatg cagcatgcgc
     8761 tatcgccaga gcagtttcag cagaaaagct tgcattcctc acggatatcg agggtgtata
     8821 caaagaccct tcagataaga atacacttat ttcagagctt acagtctcag atgcaaagaa
     8881 gcttataggt gatggcttta tcggcggagg catgcttcct aagctcaaca actgcataga
     8941 tgctatcgat aacggagttt caagagttca tattcttgat ggacgtatcg cacactgctt
     9001 actgcttgag atattcacaa acagaggaat cggaacagct attcttggag attcggagga
     9061 gaaatttaac aatgagcaat aatatggata aacagtcata cattgatgag gcagaaaagg
     9121 agcttcttca cacctacaac agattttcac ttgtacttga tcacggcgag ggagtacatc
     9181 tttatgatac agatggcaat gcatatctgg acttcgctgc aggaatagcc gtatgtgcac
     9241 ttggatacag caatgagcac cacaaggagg cattaaaggc acaggttgat aagcttttac
     9301 atacatcaaa cctctattac aatgcacctg tcatcgaggc ggcagacaag gtgcttaatg
     9361 caagtaaaat ggaccgtatt ttctttacaa acagtggtac agaggctatt gagggagcta
     9421 tcaaggctgc aaagaaatat gcctttacaa gagacggcca tgcaggtcat gagataattg
     9481 ccatgaacca ttccttccac ggaagaagca tcggagcgct ttctgtcacc ggcaatgcac
     9541 attatcagga gccgtttgag ccacttatgc cgggcgttaa atttgcagat ttcaatgatt
     9601 tagagagcgt taaggctcag gtgacagaca aaacctgcgc cattattatg gaaactattc
     9661 agggtgaggg tggtatttac ccggcaaaga aggagtttat cgagggagtg agagctctct
     9721 gcgatgaaaa ggatattctt cttattcttg acgagataca gtgcggcatg ggcagaagtg
     9781 gcgaaatgtt cgcatggcag aactatgatg tgaagcctga tatcatgacc tgtgcgaagg
     9841 cactcggctg tggtgtgcct gttggtgcat ttcttatgac acagagggtg gcagacaaat
     9901 cccttgcacc gggagaccat ggcaccacat acggcggcaa tccgttcgta ggagccgcag
     9961 taagtgcagt gtttgatgag tttaagcgtc ttgatatagt aagccatgta aaggaggttg
    10021 caccttacct ggagaagagg cttgatgagc ttgttgcaaa attcgactgt ctcacagcca
    10081 gaaggggtat gggactcatg cagggcgtgg agtgcagcct tcctgtagga aaggtctcag
    10141 cagaggcttt aaatcaaggc cttatagtca ttacggcagg cagcaatgtg cttagatttg
    10201 tgcctcctct tgttatcgaa aagagtgatg ttgacgaaat gatagaaaaa cttgaaacaa
    10261 cacttgtaat actgtcaaaa agtctgtaaa atagagagga catcgatttc tcctttgaga
    10321 ttatgatatt aatcaaagga gaccatatga aaaaaataat attattaagt gcgtttattt
    10381 taacaatagc gtttggcctt tgcggctgta aaaaggatga tgccacagtt cattttgctt
    10441 ctgaaacgga gcagactgct gtggtttcaa gtgatgtaaa cagacagact gctgattcca
    10501 aggagaagaa agcttcatcc gatggagaac aggagcagaa ggcaaaagac acagcagatg
    10561 acactgatga atctactgag tggtgcgtct atatctgtgg tcaggttgcc aatcccggtg
    10621 tatacagagt aaagccggg
//
LOCUS       DPUF01000052            3256 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_44756, whole
            genome shotgun sequence.
ACCESSION   DPUF01000052 DPUF01000000
VERSION     DPUF01000052.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_44756"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="DHV81_01865"
     CDS             complement(<1..1169)
                     /locus_tag="DHV81_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HCI93276.1"
                     /translation="MNIENKKILHTDILIIGGGTAGCYAALTIREKSDYSIIIAEKAN
                     IKRSGCLAAGVNAINAYIVKGRKPEDYVDYAKKDADGIVREDLLMTMSEGLNRVTDKM
                     EKLGLVILKDENGEYVARGNRNIKINGENMKPLLAEAVSKLTDCTVINRINITDYIVE
                     DNTIKGAYGFSIEDATAYEIRAKKVLCATGGAAGLYRPNNPGFSRHKMWYPPFNTGAG
                     YAMGIRSGAEMTTFEMRFIALRCKDTIAPTGTIAQGVGAKQVNSLGEVYETKYGLTTS
                     ERVYGTVMENLEGRGPCYLRTEGISPQQDESLRKAYLNMAPSQTLKWVEAGKNPSEQN
                     VEIEGTEPYIVGGHTASGYWVNTERETTIHGLYAAGDVAGGCPQKYVTGAMVEGE"
     gene            complement(1222..2283)
                     /locus_tag="DHV81_01870"
     CDS             complement(1222..2283)
                     /locus_tag="DHV81_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HCI93277.1"
                     /translation="MYVEMKNIYKKYGDFLASDNVSFGVEKGKLVALLGPSGSGKTTL
                     LRMIAGLETPNSGDIYIDGKRVNDIPASKRGIGFVFQNYALFRYMTIYDNVAFGLELQ
                     KMPKKLIKERVTKLLEMTGLSGLEKRYPNQLSGGQRQRVAFARALAPNPQVLLLDEPF
                     AAIDAKVRGELRTWLKELVEKLGITSIFVTHDQDEAVEVADEIIITNHGTIEQMGTPL
                     EIYKSPDTPFVAQFIGHTTVVENYDRLKGFDRIENADKAVIRPEFIKISKSGNLDRYQ
                     SAAEEGIVTDVVFRGNRMDVTVNINGIEVVGERSLEKDLIAVGEKVHVLIYRLYIFDE
                     EKTYLMENSEMQEQDVFYI"
     gene            complement(2356..3226)
                     /locus_tag="DHV81_01875"
                     /pseudo
     CDS             complement(2356..3226)
                     /locus_tag="DHV81_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568202.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease"
     assembly_gap    2578..2647
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          744 a          857 c          598 g          987 t
ORIGIN      
        1 atttcaccct ctaccatagc tcctgtaacg tatttctgtg gacatcctcc tgccacatct
       61 ccagcagcat aaagtccgtg gattgtagtc tcacgctcgg tatttaccca gtagccgctc
      121 gctgtgtgcc cacctacgat atacggctcg gtgccctcta tctccacatt ctgctcactt
      181 ggattcttac ctgcctccac ccacttgagt gtctggcttg gtgccatgtt gagatatgcc
      241 tttcttaatg actcatcctg ctgtggtgag atgccctctg tccttaagta gcaagggcct
      301 cttccctcaa gattttccat gaccgttccg tatacacgct ctgatgtggt gagaccatat
      361 tttgtctcgt aaacctctcc aagagaattg acctgctttg ccccgactcc ctgtgcaatt
      421 gttcctgtcg gtgcgattgt gtccttgcat ctgagtgcga taaatctcat ctcaaaggtg
      481 gtcatctcgg ctccgcttct tatgcccatc gcatatccgg cgcctgtatt aaacggtgga
      541 taccacatct tgtgccttga aaagccggga ttgtttggtc tgtataaacc ggccgctccg
      601 cctgtagcac acagcacctt tttagccctg atttcatagg ctgtagcatc ctcgattgaa
      661 aatccgtatg caccctttat ggtgttgtcc tcaactatat agtccgtgat attgattctg
      721 tttatcactg tacagtccgt aagctttgaa acagcttctg ccaaaagcgg cttcatgttc
      781 tcgccgttta tcttgatatt tcggtttcct cttgctacat actcaccgtt ttcatccttt
      841 aatatgacaa gtccaagctt ttccatcttg tccgtcacac ggtttaagcc ctctgacata
      901 gtcatgagta agtcttccct tactatgccg tcagcatcct tttttgcgta gtctacatag
      961 tcctcaggct ttctgccctt tactatgtat gcatttatcg cattgacgcc tgctgcaagg
     1021 catccgctac gctttatgtt tgccttctct gcaattataa tcgaataatc cgatttctca
     1081 cggatggtga gtgctgcata acagcccgcc gtgccgcccc ctattatgag gatgtccgtg
     1141 tgtaggattt ttttattttc tatattcatt tatctccctc tgcttcctct ttcaatcaca
     1201 agctttagac tattcttgta tctaaatgta gaatacatcc tgctcctgca tctcgctgtt
     1261 ttccatcagg tatgttttct cttcatcaaa tatatacagc ctgtaaatca gcacatgtac
     1321 tttctcgccg actgctatca ggtccttttc aagtgagcgc tcaccgacta cctcgatgcc
     1381 gtttatgttg accgtgacat ccatcctgtt tcctctgaaa accacatctg taacgatgcc
     1441 ctcctctgcc gctgactgat agcggtccag gttgccactc tttgatattt tgataaactc
     1501 cggtctgatt actgccttgt cggcattttc tatcctatca aagcccttaa gcctgtcata
     1561 gttttcaaca actgtggtgt gtcctataaa ctgcgccaca aaaggtgtat caggtgattt
     1621 gtatatctca agaggtgtac ccatctgctc tatcgtgccg tggtttgtga taatgatttc
     1681 atctgccacc tcaacagcct catcctggtc atgtgtgacg aaaatgcttg tgatcccaag
     1741 cttttcgacc agctccttaa gccatgtacg aagctcacct cgcacctttg catctatcgc
     1801 ggcaaatggc tcatccagta agagcacctg tggattcggc gccagcgccc ttgcaaatgc
     1861 cactctctga cgctgtcctc cggaaagctg attcggatat ctcttttcaa gccccgataa
     1921 gcctgtcatc tcaagaagct ttgtaacacg ctcctttatg agctttttcg gcatcttctg
     1981 aagctccagc ccaaatgcca cattgtcgta tattgtcatg tatcgaaaca gcgcatagtt
     2041 ctgaaacaca aagcctatgc ctctcttcga ggccggaata tcattcactc gctttccatc
     2101 tatatatatg tcacctgaat ttggtgtctc aagccctgct atcatacgaa gaagtgttgt
     2161 cttgccactg ccggacggac caagcagcgc cacaagcttt cccttttcca ctccaaagct
     2221 cacattatcg gatgccagga agtctccgta ttttttgtat atatttttca tctcaacgta
     2281 catatacgtt atctccttcc tgcttttccc atgcagcaga ttttattagt gtaaatcata
     2341 ttttccaagt atatctcagt gtaatttctt aaatttatgc tcaactatat ttcttgcaaa
     2401 aagaatgatt aatgcaataa ttaacagtat cgacgataca gcaaatgccg atgttatcga
     2461 gctgtctgtg cccgacatat acaaggcatc aatctcaagc ggcagtgtaa atgtctcgcc
     2521 tcttgtcttc gagagcgcat ttaccgcacc gaactcgccg agggctctcg ccgtacannn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnaaa tatcgtaaag ccggatgctc ccatcagcgc tgcggcttct tcctcatcct
     2701 ttccctctgc attgagcacc ggtatgattt cccttgacac atacggaaac gtgacaaata
     2761 tcgtagcaag cacaactccc ggtattgcaa aggtgattct cacattcgtg ccgaatatct
     2821 cattgaacca tctgataacc ggataaaacc acccaagccg tccgaaggtc ataagatacg
     2881 caagtcctac gatgaccgga gaaatagaaa acggtatatc aataagtgtt gcaagcacct
     2941 gttttccttt aaacgaaaac ctggtcaaca gccacgctgt ccatattcca aagaaggtat
     3001 tgataactac cgcaaaaact gttgctatca aagtcacctt gagtgctgac agcacgtagc
     3061 ttgtggagat agctttcaga tagaatccaa gcccctcctt taaggcactt gccatcacag
     3121 aaaaaagcgg aactacaagc atgacaaata tgaagagcac cgtgacagtg acgagtatta
     3181 tctttgtgat cttttttctt ctttcaagct ttacctgttc ggtcatcgca ttgtgctcct
     3241 ttcctgtttg tgtgca
//
LOCUS       DPUF01000053           19169 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1047, whole
            genome shotgun sequence.
ACCESSION   DPUF01000053 DPUF01000000
VERSION     DPUF01000053.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 19169)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19169)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19169
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1047"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /gene="fusA"
                     /locus_tag="DHV81_01880"
     CDS             <1..1530
                     /gene="fusA"
                     /locus_tag="DHV81_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCI93278.1"
                     /translation="YIYNDEKGDDITVTDDLGDMADQAAEYRTELIEKICELDDDLMM
                     QYLEGEEPSVEDMKKVLRKATCECTAVPVCCGSAYRNKGVQKLLDAIVEYMPAPTDIP
                     SIKGTDLEGNEIERHSSDDEPFSALAFKIMADPFVGKLAFFRVYSGKCKAGSYVLNAT
                     KDKKERIGRILQMHANKRQELDEVYSGDIAAAVGFKFTTTGDTICDEQHPVILESMEF
                     PEPVIELAIEPKTKAGQGKLGEALAKLAEEDPTFRAHTDQETGQTIIAGMGELHLEII
                     VDRLLREFKVEANVGAPQVAYKETFTKAVDQEYKYAKQSGGRGQYGHCKVRFEPMDAN
                     GEELFKFDSEVVGGAIPKEYIPSIGEGIEEATKAGSLGGFPVVGIHATVYDGSYHEVD
                     SSEMAFHIAGSMCFKEAMAKAAPVLLEPIMKVEVTMPEEYMGDVIGDVNSRRGRIEGM
                     DDLGGGKIVHAYVPLAEMFGYSTDLRSKTQGRGNYSMFFDRYEPVPKNVQDKVLANHA
                     K"
     gene            1741..2952
                     /gene="tuf"
                     /locus_tag="DHV81_01885"
                     /pseudo
     CDS             1741..2952
                     /gene="tuf"
                     /locus_tag="DHV81_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850603.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     assembly_gap    1986..2016
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2727..2880
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3211..3648)
                     /locus_tag="DHV81_01890"
     CDS             complement(3211..3648)
                     /locus_tag="DHV81_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCI93279.1"
                     /translation="MNVRKIAKATGISATTLYSIIQKDSNIRFDFALRLANELQIDVN
                     EICSASPFSDTIKEEEIYPTLPDGLNGVLDASRVKTYLKNSMYPLMYLFGKNSMPDVD
                     NLLTSFYQLDDEARQEVVETIRLKLQYHRDPKRAEQIKQIKGW"
     gene            4001..5128
                     /locus_tag="DHV81_01895"
                     /pseudo
     CDS             4001..5128
                     /locus_tag="DHV81_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515522.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
     assembly_gap    4494..4606
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5146..6405
                     /locus_tag="DHV81_01900"
     CDS             5146..6405
                     /locus_tag="DHV81_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI93280.1"
                     /translation="MNKHELYHNHNYVYLLTSNIINRCGDSLDTILFTWLVYLLTNSA
                     GWSAIIFGINQATSVIIQPFIGPLVENMNKKKVLVLSDIARVLLVLYILYVYTQNMLS
                     PLILVFMTISISLIEAFHMPAGVAVIQHVLPNEHYDKGVSVNVSCTKIAVLVGTGLSG
                     IVISSFGIGISLAIDALIFLISALLIVKIEFSVQEEKKTNRIIGREYLISLRDGFSYV
                     FDHKKLLKLCFLCILINSAVVPFDALLAPIAREMFSGDAKIVSLLSVSVTIGTILGSL
                     TFAKMKEEKKNNTLVTFCGIVLGVYYVFIAFVSKYIANPITQKLLLLIGSIIIGAALG
                     LMITYVQVKFVKEVTKEYIGRVSAIRFSLTYACAPVVSFVISIIYKVTVVDTSSLFVG
                     FGIVIIILFLLANKITFSPLKKGDKSY"
     gene            6825..7346
                     /locus_tag="DHV81_01905"
     CDS             6825..7346
                     /locus_tag="DHV81_01905"
                     /inference="COORDINATES: protein motif:HMM:PF13302.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCI93281.1"
                     /translation="MMKIETERLYIRHFVNEDASICFEGWGTDEHLGDFIMGYPMNMD
                     QMNSFVNAMVKNTNAWVIVEKESGACIGYISFDIPYQELRIGEIGYVIGEKWHGKGYG
                     TEAVKKMISEYLSKDRLYLIEAKCNIDNKPSLNLLEKVGFIKDGVLRKRRIDKNTGEY
                     RDLVVYSITTNDK"
     gene            7380..7904
                     /locus_tag="DHV81_01910"
     CDS             7380..7904
                     /locus_tag="DHV81_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93282.1"
                     /translation="MKNEFLEALGSNNANNNTDLSLYSRFVGNWSFTMTTYDEEGKIE
                     DTKEGEWLFSYVMDGYGIQDVFICPKRGEWTEEDTLYGDYGTTIRVPMIDNSKKWNIV
                     YVSKWSVDRLIAEELENDIIQTGINKDANDTTIWQWNFKNIEKDTFSWESIYSKDCGK
                     TWKYATKVEAKREM"
     gene            8161..8463
                     /locus_tag="DHV81_01915"
     CDS             8161..8463
                     /locus_tag="DHV81_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HCI93283.1"
                     /translation="MVKHIILWTLKDEYTQAQKKEIRAGIKEGLEGLKGQIPGMIDIS
                     VRTDYLPTSTVDVMLDTTFEDADALAAYAKNPKHVAVADTRVRPFTANRACMDFEV"
     gene            8626..9948
                     /locus_tag="DHV81_01920"
     CDS             8626..9948
                     /locus_tag="DHV81_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCI93284.1"
                     /translation="MGEEAKSKIMEMPLQQRLKYGYKKVLDMLLVSGGISIVAVLLVY
                     FGAKANIFGAESGTVATFFLVIGLANVIIVGIVATMIAKKISDQVIDSVLEPLQQIEV
                     VAGELVNGNLHSNLEYHSDDEVGKLAHDLRKSIRTLGTYIDDIDQTMRQFADGNFNFK
                     PQVEWKGDFVGIKESIVAFEESMSDTVSGIQRAANEVSNGAEQVAASSSDLAQGATDQ
                     ASVVEELTATLASVADQVAENAEHAKEISQKVDGLGTEIMDSNGQMHEMVTSMNEINE
                     ASQEIGKIIATINEIASQTNLLALNASIEAARAGEAGKGFAVVADQVTVLAEQSANAA
                     KESAALIESSVKAVEKGMVIAGKTASQLEEVAGNSKTITDEVNGIAETLETQTDAIKQ
                     INNGVEQINDVVQTNSASSQECAAASQEMSSEAESLNELIRHLRVAEQ"
     gene            10105..11604
                     /locus_tag="DHV81_01925"
     CDS             10105..11604
                     /locus_tag="DHV81_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93285.1"
                     /translation="MSMTLKDRIPKEFYSLFRTKNMDAYMQIVVALYEENNEVYASMG
                     LTREECQIIIADTISKTGIVWQTDYNEDESDKDSGSMDNHDDYNPDSEIDVIYDQATF
                     AYTLTPSAILGRLINWGWIRSDFDEKLNTYVIAFPQYSQMYAELFKKLLVDDDSRERE
                     SILAVYSALFTYFSDPEKNNDILKNALYTSKNLGQLLSNMQDGMRAYFDELSRMKDFI
                     GIQKVLIKEINNSDSRRYAILTTTDSFYRYKEAVKELISKILNQNDDRRAELEEILSQ
                     TTPGTFERKRYEYSVEYCDKASELVYKVEHEFDLIERKYNKLIEQKTIFAKRALARIH
                     YILQEGADDEDNIVKLINLIDKSDKTDEILGALRNRMKFTRQFYNVTDDSFPGRREKG
                     RSEFVPFVIDDASAGEDDMADFVPKPLYTKKELQSFRDKNMRDGMFVTDEQTVESMED
                     LEKLLFLWQEETNERMGEDTVSLDGEITKNDGVSYSRLVISKSDSKREA"
     gene            11620..>12230
                     /locus_tag="DHV81_01930"
     CDS             11620..>12230
                     /locus_tag="DHV81_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93286.1"
                     /translation="MFEYMENVSPNEAENIRKTIQDLLRQTCILQMKCDPVTLIQRDN
                     PRYQVCLRNREFISDYLAVLDCELVHDQQEHLFRITGDGVMLEKMTLLTARIVIIMKM
                     IYRDKIMGEGLNATTTNLAEIREYGRNTNLITRKLTNQEWSDALLLMKTHQMIELPGA
                     IANLEDNTPIYIYGTVNIFCSAMDINELVRMYSDEVELIKYDN"
     assembly_gap    12231..12324
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12360..15689
                     /locus_tag="DHV81_01935"
                     /pseudo
     CDS             12360..15689
                     /locus_tag="DHV81_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741315.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    15521..15608
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15692..16156
                     /locus_tag="DHV81_01940"
     CDS             15692..16156
                     /locus_tag="DHV81_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCI93287.1"
                     /translation="MAYYIAEGAVVKGQVTMADGASVWYNATVRGDSEPIEIGRNSNI
                     QDNAVVHVDLSHSVRIGDNVTIGHSAIVHGCTIGDNTLIGMGAIVLNGARIGKNCIIG
                     AGALVTQGTDIPDGSLAFGSPAKVIRALTADEIEENHRNAMHYVEIARESLI"
     gene            16264..17677
                     /locus_tag="DHV81_01945"
                     /pseudo
     CDS             16264..17677
                     /locus_tag="DHV81_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741317.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     assembly_gap    17472..17548
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17708..18451
                     /locus_tag="DHV81_01950"
     CDS             17708..18451
                     /locus_tag="DHV81_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCI93288.1"
                     /translation="MINTEGTKVWAHRGASGYAPENTLEAFKKAEKMKADGVELDVQL
                     TKDGELVVIHDETVDRVSGEKGFVKDYTLKELKRLNVSKHMPSYDKKTRIPTLDEVFD
                     LLKNTEMTINIELKNGIVQYKNLEKKVLALVEEYDMLDRIWCSSFNHESIVRMKKLCP
                     DMRCGLLFSDIIVNPAEYASELHVDALHPATYHMQDENYIDKAHGRGLRAHVWTVNEK
                     HEMKALVKAEADAIITNYPDIAVEIVKNA"
     gene            18766..18975
                     /locus_tag="DHV81_01955"
     CDS             18766..18975
                     /locus_tag="DHV81_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93289.1"
                     /translation="MQLAAKETLRCADIVEEMFVNLHECIDKKDKNIENELKSARTLQ
                     KHYVLCGIARIGILSDDINGEVNIA"
     gene            18968..>19169
                     /locus_tag="DHV81_01960"
     CDS             18968..>19169
                     /locus_tag="DHV81_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93290.1"
                     /translation="MHRERVLKALAQLLVGVENKLHLADRRRRREDKLIERARLLEIQ
                     RAQNKTNLKDADANGKISYRIGA"
BASE COUNT         6109 a         2864 c         4536 g         5103 t
ORIGIN      
        1 tatatctaca atgatgagaa gggtgacgat atcacagtta ctgatgatct tggagatatg
       61 gctgatcagg ctgcagagta ccgtacagag cttattgaga aaatctgtga gttagatgac
      121 gatctcatga tgcagtatct tgagggtgag gagccatctg tagaggatat gaagaaggtt
      181 cttcgtaaag caacatgtga gtgtacagca gtacctgtat gttgtggttc tgcataccgt
      241 aacaagggtg tacagaagtt acttgacgct attgttgagt acatgccagc tcctacagat
      301 atcccttcaa tcaagggaac agatcttgag ggaaatgaga ttgagagaca ttcatcagat
      361 gatgagccat tctcagcact tgcattcaag atcatggcag atccattcgt aggaaagctt
      421 gcattcttcc gtgtttactc aggaaaatgt aaggcaggtt cttatgtatt aaatgcaaca
      481 aaggataaga aagagcgtat cggacgtatt cttcagatgc atgcaaataa gagacaggaa
      541 ttggatgagg tttactcagg tgatatcgct gctgctgttg gatttaaatt cacaacaaca
      601 ggtgatacaa tctgtgatga gcagcatccg gttatccttg agtctatgga gttcccagag
      661 ccagttatcg agctcgctat cgagccaaag acaaaagctg gacagggtaa gttaggtgag
      721 gctcttgcaa agcttgcaga ggaggatcct acattccgtg ctcatactga tcaggaaaca
      781 ggtcagacaa tcatcgctgg tatgggtgag cttcaccttg agatcatcgt tgaccgtctt
      841 ctccgtgagt tcaaggttga ggcaaacgtt ggtgcacctc aggttgctta caaggagaca
      901 tttacaaagg ctgttgatca ggaatacaaa tatgctaagc agtcaggtgg acgtggacag
      961 tacggacact gtaaggttcg cttcgagcca atggatgcca atggagaaga acttttcaag
     1021 ttcgattcag aggttgtcgg tggtgctatt ccaaaggagt acatcccatc aatcggcgaa
     1081 ggtatcgagg aggctacaaa ggccggctct cttggtggat tcccagtagt tggtatccat
     1141 gcaacagtat acgatggatc ttaccatgag gtcgattcat cagagatggc attccacatt
     1201 gccggatcta tgtgtttcaa agaggctatg gcaaaggccg cacctgtatt acttgagcct
     1261 atcatgaagg tagaggttac aatgcctgag gagtacatgg gagacgttat cggtgatgtt
     1321 aactctcgtc gtggacgtat cgagggtatg gacgatctcg gcggtggaaa gattgtacat
     1381 gcatatgtac cacttgcaga gatgttcggt tactctacag accttcgttc taagacacag
     1441 ggacgtggaa actactcaat gttcttcgac agatatgagc cggttccaaa gaatgtacag
     1501 gacaaggttc ttgctaacca cgcaaaataa ttgcaaatta gcaaacaatt tatagtacaa
     1561 atgatgaaat atatactata tatggtataa aaatcaaaaa taaaggcatt tccccttgaa
     1621 aaagggggat ttgctataaa atttacttga aaaaactttg attatttcgt ataatcaaag
     1681 cagtcggtag attataaaaa aagcccatgg ggcataaaag tttaaggagg acgtttcaaa
     1741 atggcaaagg ctaaatttga aagaacaaaa ccacattgta atattggtac catcggtcac
     1801 gtagatcatg gtaaaacaac tttaacagca gctattacag cagtattagc agctcgtgtc
     1861 gctggtaaca cagctacaga tttcgctaac attgataagg ctccagagga gagagagcgt
     1921 ggaatcacaa tttctacagc tcacgttgag tatgagacag aaaagagaca ttacgcacac
     1981 gttgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctg actacgtaaa gaacatgatc
     2041 actggtgcag ctcagatgga tggtgctatc ctcgttgttg ctgctactga tggtgttatg
     2101 gctcagacaa aggagcacat cctcctttct cgtcaggtag gtgttcctta catcatcgta
     2161 ttccttaaca agtgtgatat ggttgacgac ccagagctta tcgagctcgt agagatggaa
     2221 gttacagagc agcttgagga gtacggattc aatgattgcc caatcatcca gggatcagct
     2281 cttaaggctc ttgaggatcc aatgggacct tggggagaca agatcatgga gcttatggat
     2341 actgttgatt cttatatccc agatccacag cgtgatacag ataagccatt ccttatgcca
     2401 gtagaggacg tattcacaat cacaggtcgt ggtactgttg caactggtag agtagagcgt
     2461 ggtacacttc acctcaacga cgagttagag atccttggtg ttaaagagga cgttcagaag
     2521 actgttgtta ctggaatcga gatgttccgt aagcagttag acgaggctca ggctggtgat
     2581 aacatcggag cacttctccg tggtattaac cgtgaccaga tcgttcgtgg acaggttctt
     2641 gctaagcctg gtacagtaac atgtcaccat aagtttacag ctcaggttta cgttcttaca
     2701 aaggacgagg gtggacgtca tactccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 ggtcttacat tcgctatccg tgagggtgga cgtacagtag gatctggacg tgttgctaca
     2941 gttatcgagt aatacaatct taatatggat ttgttatcgt aagataacgt tgtgtctaaa
     3001 cgtaagatac ccgtagaact tcggttctac gggtttttct gtatcttaaa gagagaatga
     3061 tttgaaaaat tataagtaga gaaatagcga aatatttatc agcaagataa aatacagata
     3121 aatttaaaat tgaaaaataa gaccagtggt gccaaaacgg tgcccaaaaa gaaactcctg
     3181 cgaatgtttg ccattcacag gagtgatttt ttaccaacct ttaatctgct tgatctgttc
     3241 agcacgtttc ggatctcgat gatattgcag tttcagacgg attgtttcta caacttcctg
     3301 tctggcttca tcatcaagct gatagaatga tgtaagtaga ttatctacat caggcatact
     3361 atttttgcca aaaagataca tcaacggata catagaattt tttaagtaag tctttacacg
     3421 acttgcgtct aagactccgt tcaaaccgtc tggaagtgta ggataaatct cttcttcctt
     3481 aattgtgtca gaaaatggac tggcagaaca tatctcattc acatcaattt gcaattcgtt
     3541 tgcaagtctt aaagcaaaat caaatcgaat attggaatct ttttgaatga ttgaatataa
     3601 ggttgtagca ctgattccag ttgcttttgc aattttacgg acatttataa gtggttaaga
     3661 gatctgatgt atgcgtagaa aatggaaaat atgttacaaa taaaacgaga atttgaactc
     3721 gaaatttggg aaaaataatg aaaataactt gacatttgaa aataagagat gtattcttaa
     3781 gataaattca ataaacggga ggtggataaa aatgtttaat cttgaagaat tgagcttgga
     3841 gtttgtagag atttctgaat taaaaacctc taagaactta tttggaccag ttatgtacgg
     3901 aacaggtgtg agaatgtagt ttgtagaaaa gacttgctct atgtaaaaag aaatatagag
     3961 taggtctttt ttatttaaaa aggattatag gagagaatat atgaataaat taactacagc
     4021 tgtatgggaa ataacaatgg gttgtaatat gcgttgtaaa cattgcggtt caagttgtgc
     4081 agaagcgcta cccgatgagt tgaatacatc agaagctctt gaggtgtgtg accaattaaa
     4141 agatttggga ttaaaagtta ttacattatc tggaggggaa cctacgacac gtagtgactg
     4201 gcatattatt gcgaaaagat tagtagataa tggcataata acaagtatta ttacaaatgg
     4261 ttggttgatt gatgaaaact ttattcataa tgcgataaca tcaggaatta gaagtgtctg
     4321 tttaagtatt gatggtcttg aaaaaactca cgattttata cggagatcgg gatcattcaa
     4381 taaaagtatt aaagcattga aagaattaag aaaaaataat atttcgacat cagtaataac
     4441 ttcaataaat aaagaaaata tatgtgaatt ggaagaacta tatcaattat taannnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttaa ttgttttggc
     4621 agattgcata ggttattata gcaataaaga tataaaagta aatgagaatt ttcttaatga
     4681 taattggtct tggacaggtt gtggagcagg aaagcatgta attggtattc tgcataatgg
     4741 agatattgtt gcttgtactt ctattagaga taaaacatta gtagcgggaa atattcgtga
     4801 aaaatcttta aagtcgattt gggaaagtcc ggactctttt aagtggaata gaaattttga
     4861 tagtagtaag ttaaaaggat tttgcagaga atgtcgttat acagaacgat gcttaggagg
     4921 atgtagtaat agtcgttatt gcattaacgg ctcatttgaa agtgaaaata gatactgtgc
     4981 atataatgtt gaaatgaaaa aatggcaaaa atatttggaa tcacttaatg atatatcaga
     5041 aattgataat gttatagaaa aagcagcggc tttaaaacat caggatcttt ataagatagc
     5101 ttgcgaaatt aaacaatcta aattatgatt agagaggtaa caaatatgaa taaacatgag
     5161 ttatatcata accacaatta tgtttatctg ctgacttcta atattattaa ccgctgtggt
     5221 gattcattag atacaatact ttttacatgg ttggtatatt tacttacaaa tagtgcaggg
     5281 tggtctgcga ttatttttgg tattaatcaa gctacttctg taattattca accatttata
     5341 ggtccattag tcgagaatat gaataaaaag aaagtgttag tactttcaga tatagctcga
     5401 gttctgttag ttttgtatat attatatgta tatacacaaa acatgctttc accattaata
     5461 ttggttttta tgacaatatc catttcatta atagaagcat ttcatatgcc agcaggggta
     5521 gcagttattc agcatgtact tcctaatgaa cattatgata aaggtgtaag tgtcaatgtg
     5581 tcttgtacca aaatagcagt attagttgga actggcttgt caggcatagt gattagttca
     5641 tttggaatag gtatttcttt agcgatagat gctttgatat ttttaatatc agcgttgctt
     5701 atagtaaaaa tagaattttc tgttcaagaa gaaaagaaga caaaccgaat aattggcaga
     5761 gaatatttaa tttcgcttag agatggattt agttatgtat ttgaccataa gaagctatta
     5821 aaactatgtt ttttatgtat tctaattaat tcagcagtgg ttccatttga tgcactgctc
     5881 gccccgattg caagagaaat gtttagtgga gatgccaaaa ttgtttcatt actaagtgta
     5941 agcgtaacta ttggtactat tttgggatca ttgacttttg caaaaatgaa agaggagaaa
     6001 aaaaataata cacttgttac attttgtgga attgttttag gtgtatacta tgtttttatt
     6061 gcatttgttt caaaatatat tgcaaatcct ataactcaaa aattactgtt gctaatcggt
     6121 tcgataataa ttggtgcagc attgggatta atgattactt atgtacaagt gaaatttgta
     6181 aaagaagtta ctaaagaata tataggacgt gtttctgcaa ttcgattttc acttacatac
     6241 gcatgcgcgc cagtggtatc ttttgtgatt agtattatat acaaagttac tgttgttgat
     6301 acaagttcac tttttgtggg atttggtatt gtaataataa ttctttttct tttggcaaat
     6361 aagattacgt tttctccttt gaagaaaggc gataaaagtt attagaaatt atttatatga
     6421 aaaactggct gctttttggc tataaattaa agctgataga gaagagtata aacagcagaa
     6481 gagaagcttt ttgactgagg cttccggtgc caaaacggtg ccaagaaatc aggcaaataa
     6541 aaataaatag tacaaatcag tcgtttttca ctgtattttg gagagaaaac cataaaaaat
     6601 atgaggtaaa atgatattaa agtatcgtga gggtggacgt acagtagaat ctggacgtgt
     6661 tgctacagtt atcgagtaat tacttgttag tagtgtaaat atctagtaaa ttcaagggtt
     6721 tccgagattt ctcggaaacc cttttttaca gtttaaaagt atgttatagt aggattaatt
     6781 gatgtagtgg tgccaagtca taatgtagat ggaaaaggat ttttatgatg aaaatagaaa
     6841 cagagcgatt atacatccgc cattttgtga atgaagatgc ttcaatatgc ttcgagggat
     6901 gggggacaga tgagcatttg ggtgatttta taatgggata tccaatgaat atggatcaga
     6961 tgaattcttt tgtaaatgct atggtgaaaa atacgaatgc atgggtaatt gttgaaaaag
     7021 aaagtggtgc atgtatagga tatatatctt ttgatatacc atatcaagaa ttaaggattg
     7081 gtgaaattgg ttatgttatt ggtgaaaagt ggcatggtaa aggttatggc actgaagctg
     7141 taaaaaagat gatttcagaa tacttgtcaa aagatagact gtatctcatt gaggcaaaat
     7201 gtaatataga taataaacct tcgcttaatc tgcttgaaaa ggtaggtttt attaaggatg
     7261 gggttttaag aaaaagaaga attgataaaa acacagggga atatagggat ttggttgttt
     7321 attcaattac tacaaatgat aaataagcaa catgtttatc gggaggagag tgtacgctaa
     7381 tgaaaaatga atttttagaa gcattaggaa gcaataatgc gaataataac acagatttat
     7441 cattgtatag tagatttgtt ggaaattggt cgtttacaat gactacatat gatgaagagg
     7501 ggaagattga agatacaaaa gaaggtgagt ggcttttttc atatgtcatg gatgggtatg
     7561 gaattcaaga tgtcttcatt tgtcccaaaa gaggagaatg gacagaggaa gatactttat
     7621 acggagatta tggaacaaca atacgagttc caatgataga taattcaaaa aagtggaata
     7681 tagtgtacgt ttccaaatgg agtgttgata ggttaatcgc cgaagaattg gagaatgata
     7741 ttatacaaac agggattaat aaagatgcga atgatacaac tatatggcaa tggaatttca
     7801 aaaatattga gaaggataca tttagttggg aaagtatcta ttcaaaagat tgcggaaaga
     7861 cgtggaaata tgcaacaaag gtagaagcta aaagagaaat gtaaattaaa tgatttgaat
     7921 aataaatgta ttctaaactg tacctaatat gttttctgcc acaaaaggtg caaaatgggg
     7981 taggaaatga agtgatgggg tagaatatgt aattgacata gcggtctgaa tggcatatta
     8041 ttcatacaaa ggtctccatt caccttatgg ttttgaacta tagaagagat gaacattaaa
     8101 gaaattatta tgttatagtt tggactggtc gaaagacctg aaaaactcag gagaaacaca
     8161 atggttaaac acattatttt atggacactc aaggatgagt acacgcaggc acagaagaag
     8221 gaaatacgtg ccggcataaa ggaaggctta gagggactta aagggcagat tccgggaatg
     8281 atcgacattt cagtacgcac agattatctt ccgacatcta cagtagatgt gatgcttgat
     8341 actacatttg aggatgctga tgcacttgca gcgtatgcaa agaatcctaa gcatgttgca
     8401 gtggcggata ccagggtgag accatttact gcaaataggg catgtatgga tttcgaggtg
     8461 taataagcgg gaaattagtg tatttgacta taaatcattt ccgaaattag gcgatatgtc
     8521 aaaaatgacg aatattgtca aattatcatt gaaatagtac tatggtaatg atataataat
     8581 cttactgtat agaacgaaac ctaaatgaga aagggagaat tacatatggg tgaggaagct
     8641 aaaagcaaaa taatggaaat gccacttcag cagagattga aatatggcta taagaaagta
     8701 ttggatatgc tgcttgtatc gggaggtata tcaattgttg cagttttatt ggtttatttt
     8761 ggtgcaaagg ctaatatctt tggtgccgag tcgggaacag ttgctacatt tttcctggtt
     8821 ataggactgg ccaatgtaat aatagttgga attgtagcta caatgattgc gaaaaaaatc
     8881 agtgatcagg ttattgattc tgtgcttgag ccgttacagc agattgaggt agtggcagga
     8941 gagcttgtta atggaaatct tcacagcaat ctggagtatc attcagatga tgaggttggc
     9001 aaacttgcac atgaccttag aaagtctatc aggacacttg gcacatatat tgatgatatc
     9061 gaccagacta tgagacagtt tgcagatggc aatttcaact ttaagccaca ggtggagtgg
     9121 aaaggcgatt ttgtaggaat taaagaatca atcgttgctt tcgaggagag catgtcagat
     9181 acagtcagcg gtatccagag agcggcaaat gaggtttcaa atggtgctga gcaggtagct
     9241 gccagctcaa gtgatctggc gcagggtgca acagaccagg cctctgttgt tgaggagctt
     9301 acagccacac ttgcttctgt agcggatcag gtagctgaga atgccgagca tgcaaaggaa
     9361 atcagtcaga aggttgatgg actcggaact gagattatgg atagcaacgg acagatgcat
     9421 gagatggtta catctatgaa tgaaatcaat gaggcatctc aggagattgg taaaatcatc
     9481 gcaacaatca atgagattgc atcacagaca aaccttcttg cactcaatgc ttctatcgag
     9541 gcagccagag caggagaggc aggaaaaggc tttgcagttg tagcagatca ggttactgta
     9601 cttgctgagc agagtgcaaa tgctgctaag gagtcagcag ctcttattga gtcttcagtc
     9661 aaggctgttg aaaaaggaat ggttattgca ggcaagacag catcacagct tgaggaggtt
     9721 gcaggcaatt ccaagactat cacagatgag gttaatggaa tcgcagagac acttgagaca
     9781 cagacagatg ctatcaagca gattaataat ggcgtagagc agattaacga tgtagttcag
     9841 actaactcag cttcttcaca ggagtgtgca gctgcaagtc aggagatgag cagtgaggca
     9901 gagagcttaa atgagcttat acgtcatctg agagttgctg agcagtaagc atattatatt
     9961 gaatagaatt attgatggtc acaatcaaga gtgatttgac ggtaaccgga tgttattgtt
    10021 aagactgaga tgtgaagcag caatattaat atccccacgt ttatttccat gtatatggcg
    10081 aaaaacgtgg ggatatgtta ttatatgagt atgacattga aagatagaat accaaaagaa
    10141 ttttatagct tattccgcac gaaaaatatg gatgcatata tgcagatagt tgttgcgtta
    10201 tatgaggaga ataacgaggt ctatgcatca atgggactga cacgtgagga gtgtcagata
    10261 atcatagcgg ataccatatc aaaaacaggc attgtatggc agactgatta taatgaagat
    10321 gagagtgata aagatagtgg cagcatggat aatcatgatg attataatcc tgattccgaa
    10381 attgatgtta tatatgacca ggccactttc gcatacaccc tgacaccatc agctatattg
    10441 ggcaggctta taaactgggg ctggatcagg agtgattttg atgaaaagct caacacatat
    10501 gtgattgcat ttcctcagta cagccagatg tatgctgagc tgtttaaaaa gctgcttgtg
    10561 gatgatgaca gcagggagcg cgagagcatt ctggctgttt acagtgcgct ttttacatat
    10621 ttttcagatc cggaaaaaaa taatgatatt ctgaaaaatg cgttgtatac gtcaaaaaat
    10681 cttggtcagc ttttatcaaa tatgcaggat ggtatgaggg catattttga tgagctgtca
    10741 cgtatgaagg attttatagg tatacagaag gtactgatta aggaaattaa taacagtgat
    10801 agcagacgtt atgccatcct tacaaccacg gatagctttt atcgttacaa ggaggctgta
    10861 aaggagctta taagcaaaat tcttaatcag aacgatgaca gaagggctga gcttgaggaa
    10921 attctttcac agactacacc cggtacgttt gagagaaaac gctatgagta cagcgtggag
    10981 tactgtgata aggcaagtga gcttgtttat aaggtggagc atgagtttga cctgattgag
    11041 agaaagtaca acaagcttat tgagcagaag acgatttttg caaagagggc gcttgcaagg
    11101 atacactata tcctgcagga aggcgcagat gacgaggata atatagtaaa gctcattaac
    11161 ctgatagata agagtgataa aacagatgag atacttgggg ctcttcgaaa cagaatgaag
    11221 ttcaccagac agttttacaa tgtaacggac gacagttttc cgggaagaag agagaaggga
    11281 agaagtgagt ttgttccgtt tgttattgac gatgcttcgg caggcgaaga tgatatggca
    11341 gattttgtgc caaagccatt gtataccaaa aaggagcttc aaagcttcag agataaaaat
    11401 atgcgtgatg gcatgtttgt gacagatgaa cagactgtag aatccatgga ggatctggaa
    11461 aagcttctgt ttttatggca ggaggagacc aatgagcgca tgggtgagga tacggtgagt
    11521 ctggatggag agataacaaa gaatgatggt gtaagctact caaggcttgt aatatccaaa
    11581 tcggactcta aacgagaagc atagaaaaga gagcaatgta tgtttgaata tatggagaat
    11641 gtttcgccaa atgaggcaga aaatattaga aaaactattc aggatctgtt gaggcagacc
    11701 tgtattttgc aaatgaagtg tgaccccgtt acattgattc aaagagacaa tccaaggtat
    11761 caggtgtgtc ttagaaacag agagtttatc tctgattatc tggctgtgct tgactgtgag
    11821 ctggttcatg accagcagga gcatcttttc aggataacgg gtgatggtgt catgctcgag
    11881 aagatgactc ttcttactgc aaggattgtc attataatga agatgatata cagggataaa
    11941 atcatgggcg aggggcttaa tgcgacaaca acgaatctgg cggagattcg tgagtatggc
    12001 agaaatacca atctgattac cagaaagctc actaatcagg aatggtcaga cgctcttttg
    12061 cttatgaaaa cacatcagat gattgaactg ccgggggcta ttgcaaacct ggaggacaac
    12121 acaccaatct atatttatgg cacagtaaat atattctgct cagccatgga tataaatgag
    12181 cttgtgcgca tgtacagtga tgaggtggag ctgattaagt atgataacga nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnaccaat tgagtcagtc taagaagata tttaccagaa
    12361 tgtgtctaaa caactggggc ggaataaatc acaaggtttt gcaatttaat gagtatgtca
    12421 atttgttcag tggtaagagt ggctcgggaa aatcaaccgt catggatgcg attcaggtaa
    12481 tcctctacgg cagcttcagc cccacattct tgaacaaggc tgcagatgat gcaaaaaaca
    12541 ggagaagtgt tctgagctat ttgaggggtg agcagaagga tggttccgca aatcgtggcg
    12601 actgtgattt ttgttcggtc atagcgcttg agatagagga tacaggcagt catacgttta
    12661 catgtatagg aatagccttt gaggtacgaa aaaacgattc tgagattaag agatttgtgt
    12721 acttcagtca ttcgggcagg atgccggagc cttcatatgt gacagcggat ggcttttgct
    12781 attcaaataa tgatattaaa aagcttgtaa atactcgctc acagtcagct gacagacgcg
    12841 ccagagggga tgtaaaccgt atttacgcat cgaaggaggc atatcttggc acattatatg
    12901 atgttatttt aggatatatt gacccgaatc gttttatcac aatggaaaaa agtgctattg
    12961 cactcagaat gacaaatgga acaggccagt ttatacgtga ttatatgttt ccaaagagtg
    13021 actcgggcac gatagagact atcagtgagc agctcgatgc atacagacaa atcagggaaa
    13081 aaatagagga tatgcgcaag cgtattgagc ttctcaagga aataaaggag tcagggcagg
    13141 aactggtaaa tatccaaagc gatattgtca tggcagagac aatgatcagg tgcatagata
    13201 tcgaggatat gcgtgcaaag attcaggcag ctgaggatga taaaaagcgt atcgaagaag
    13261 aacaggacag gctttcagaa aaagcagagg atatagccgg gcagcaggca gatgtgcagc
    13321 aggagtatat ccaggtatgt gcagatttga aatcgagcga cttaggaagc aaaaaggatc
    13381 agcttaggga gcttgagaag agaagtctca tgcttaccga caacagccgt cagtggatta
    13441 aaatcacaaa cggactaaag gcctgggatg aggatgaggt catcacagat tatgtgagca
    13501 atgcagtttt aaacctcatt gaagagattg tggatggcga tgtcacagag gcaaaatgtc
    13561 aggagcttca tctaaagctt gagtctataa agaaggatat cgaggatgag ctcgaggaca
    13621 atacttcgca gaaaaatgag ataacaaaag agcttaaaga gaaaaaacgt ctggtcgatg
    13681 atatgaagaa caaccgcaag tcgtacagtg agagcctgcg ttcggcaaga gctgcgctgc
    13741 agagggactt aagtgataga tttggcgagc ctgtgaaggt acaggtattt gcggatatgt
    13801 ttgatgtaca ggacgaggaa tggaagaatg ccatagaagg acgtatgggc aggctcaagt
    13861 tctcacttat cacagagccg cgttatgcgc atactgctgc tgttattttc agagacatga
    13921 agcagtttga ggatgtcgac ctcatcaatt ccaaggctat catggacagt gaaccaaagg
    13981 ccatggatgg ttcgctctat gaggcggtgg atacaaagga aaagtatgta gatgtctgcc
    14041 tgaaaagata tctgggacat attataaagt gccggagtgt tgacgagctt gagcaggtac
    14101 gagatggagt tacaccggac tgctactcat acagcaactt tattttcaga catctgaaga
    14161 agcgcgatta tactacaaat gcctgcatcg gaggcagggt atccaaggca agacttgctg
    14221 agtatgagac agatgtggag aggcttgaga aggaccggat acagcttatg ggtattattt
    14281 caagtctcaa ggcacagaga ggttttgagt gcttaaaggg ggagccggag tattttgtgg
    14341 gcctttctga ggcgtcagat gagcttaaag cagttaattc gcagaaaaat gagctcgaag
    14401 agattatcag gacaatggag aatgggcagt acaaggagct aaaggagaaa aagaggcttc
    14461 ttgagaaaaa catcgatgca cttaagagtg ctttttcact gaatcagcag cagataaatg
    14521 aaaagataag ccagcatgag aaaatcatag gtgcaaacga acagcgaaga gcacagctgg
    14581 atgatatgtt acagggctat gttgccagag aggagcttga gaagcaggta caggatatgc
    14641 tgcagcatag ctcaggagca tcggtcaaga gcaaacagcg tgagaagctt attgagctgc
    14701 aaggcgcaga cggaaatggt ggcaggcagc agtcgtgtgt ggagacgttg cagactgccc
    14761 gctacaggta tataaaggag tttccgtcag gtcagttcag cggagccgag aagtcaaata
    14821 aggcatatga ggagctgctg gagaagtacg agaaggatta tgagccggca tatgagagtg
    14881 agtttgagga gcagtgcaag gtgatttata aaagtctcag agagaatgtc atcggtacaa
    14941 tccacggtga tatcaaggct gcaaagcgcc atgcctatga gataaacagg cttcttaggg
    15001 agactaattt ctcagacagt acctatcaga ttaagataga gccggcaaag aacgaaaacg
    15061 gtcagttcta tgatatgctt atggcggaag aactggacag taaaaatccg gataatggcg
    15121 gaatagctgg acagataagc tttggtgagg atgattttta taagaaatat gagcagaaaa
    15181 ttaaactttt gacagacaag ttcatgccgc caagggatga ggatgagcat ctgcgaatgc
    15241 agaagcgcaa ggaaatggag cagtatgcgg attaccgcaa ttatctgtca ttcagcatgt
    15301 atgagcaggt tacggacgaa aacggccgtg tcatcaggga aaactttgta gatgacatgg
    15361 caggacgtga ttcgggcggc gagggacaga accctaagta tgttgcgctt cttgcgggct
    15421 ttgcgatgct ttatatgcag cagagcaatc gtgattcaaa gattaagctg gtgcttttgg
    15481 atgaggcatt ttccaagatg gatcaggagc gaagtgcggt nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnta tggcttcagg cggcataata atcagatttc tatgatgcat atagacaagg
    15661 gcgattatct gaaattgatg gagggataga tatggcatat tatatagcag aaggtgccgt
    15721 tgttaagggt caggtgacaa tggctgacgg agcaagcgta tggtataatg caactgtaag
    15781 aggcgattca gaaccgatag aaataggcag aaattcgaat atacaggaca atgcagtggt
    15841 acatgtggac ttaagccaca gtgtcagaat cggtgataat gtgacaatag gacacagcgc
    15901 aattgttcac gggtgtacta taggtgataa tacactcatc ggcatgggtg ccatcgtgtt
    15961 aaatggagca aggattggta aaaactgcat aataggtgcg ggcgctcttg tcacccaggg
    16021 caccgatatt cctgacggaa gcctcgcatt tggttctccg gcaaaggtga tcagggcact
    16081 gaccgctgat gagatagagg agaatcatcg caatgccatg cattatgtgg agattgcgag
    16141 ggagtcgctg atttaatatt ggacggatta tttacataaa ctttgcaaat gggtggtagc
    16201 ggtttttaca taaaaaaaat aaaatcgtaa tctgggacat acaaaacagg gaaggtataa
    16261 tttatgaaat caaaagaaga caatacacaa aagaaaacca aaaagctctc atcaaaggag
    16321 ctctcatggg tattatatga tgtgggaaat tccgcataca cactgcttgc gtgtgcgctc
    16381 ataccgatat ggtacaaaag cctttccgtt ggagatgggg cagggcagat ttcatctgac
    16441 agggcgactg catattatgc ggtggcaata gctgtgatga cagttgtatc agcacttatc
    16501 gggcctgtct gtggtgcgat agcagatcat atgcgcatca aaaaggctat tttttccacg
    16561 acagtagttg taggagtgtc ggcatgtata cttaacggtt ttacactcac atgggtgctg
    16621 tttcttgtta tctatgtgct tacaaagata ttttacaatg catcgctggt gttttatgac
    16681 tcgatgcttg tcgatgtgac gacaaaggat agaatggacg aggtgtcttc ctacggctat
    16741 gcctggggat atctgggctc gtgtgtgccg tttcttgttt cacttgcagc gtacatctgt
    16801 ggaccggata tgcttggata catatcaaac cgcctatcga tgataatagg ctttgcggtt
    16861 acaggaattt ggtggtttgt tgttacaatt ccattgttta agagctataa acaggtgaat
    16921 tatgtatcgt atgcagcaga caaagatatt cataagaatt ttgaaaatga tgtatttata
    16981 agagacaata tcaaggagaa aaacaaaaca aacaaaaatc cgggagtcct aagacttata
    17041 gctgacgcat ttgcacagat ttttggaaca atcaaaaaaa tagctacaaa agataaaaaa
    17101 gtaggactgt ttttggttgc ttttttcctt tacatagatg gtgtaggtac tataatagat
    17161 aattgtataa acataggaac tgatttaaag ctcgattcag tgggacaggt agtgttttta
    17221 ctttttacac agatagtggc atgcatcggt tcacttgttt ttggaagact gtcacagaca
    17281 tacaaaacta caacgctttt gtatgtctgc attgcgggat attttgcagt ttgtctatat
    17341 gcacttacac tgcatgatct gattggcttt ggaatcatgg catttggtgt gggatgcttc
    17401 cagggctcat tgcaggcgct gtcacgctca tatttttcaa agattattcc tcctgagaac
    17461 tcgggagagt annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gtcagtaaag cttgcaggcg gcacgataaa
    17581 tgtggcagtt gcaactatgg caatattctt tgcagtggga tttgtatttt taaggcttgc
    17641 agacagatat gatgcaccga ggcatgaaaa taactaaaat aaaaagtaac gaaagaaggc
    17701 ttaaagcatg ataaacacag agggaacaaa ggtctgggca cacagaggag catccggata
    17761 tgcaccggag aacacacttg aggcattcaa gaaggcagaa aagatgaagg ctgacggcgt
    17821 ggagcttgat gtgcagctca caaaggatgg ggagcttgtg gtcatacacg atgagaccgt
    17881 ggacagagtg agtggagaga aaggctttgt aaaggactat acactcaagg agttaaagcg
    17941 cctcaatgtg tcaaagcaca tgccgtccta tgacaagaag actcgcattc caacactcga
    18001 tgaggtgttt gacctgctca aaaacacaga gatgaccata aatatcgagc tcaaaaacgg
    18061 tatcgtacag tacaaaaatc tggagaaaaa ggtactggcg ctcgtggaag agtatgatat
    18121 gcttgaccga atctggtgct catcattcaa tcacgagtcg attgtgcgca tgaaaaagct
    18181 ttgccctgat atgaggtgcg gtctgctttt ttctgacatt atagtaaatc cggcagagta
    18241 tgcaagcgag cttcatgtgg atgcgcttca tccggcaacc taccatatgc aggatgaaaa
    18301 ctacattgac aaggcgcacg gaagaggact tagagctcat gtatggactg taaatgaaaa
    18361 gcatgagatg aaggcacttg tcaaggcaga agcagatgca attatcacaa attatccgga
    18421 tattgctgtt gaaatagtta aaaatgccta aaagatatat aaaatgcaca caaaattaat
    18481 ttatgtaaag ctgagtaaag aaatgtaaag tttttaccat gattttacat aactgcgttt
    18541 tggactttac attagcttga tactattaac ttgttgaata gttattgatt aattttaata
    18601 atttatcatg ctaatggaga gattaattat gttgaaaaag aaattactaa agaaagcggt
    18661 cgtcatagga atgatggcaa cgatgataac aggctgttca caagaggtac aaactaccaa
    18721 tgtggacggc cttaattcat ataaaaatgc gtcaaagagg tggtaatgca gcttgctgca
    18781 aaggaaactc tcagatgtgc tgatattgtt gaagaaatgt ttgtaaattt gcatgaatgt
    18841 attgacaaaa aagacaaaaa tatcgaaaat gaacttaaga gtgcacggac attgcagaag
    18901 cattatgtgc tgtgcggtat agcgagaatt ggaatattat cagatgatat caacggagag
    18961 gtaaatattg catagggaaa gagttttgaa ggcacttgca cagctgcttg tcggggtgga
    19021 aaataagctt catctggcag acagaaggag acgccgtgag gataaactga tagagcgggc
    19081 aaggctgctt gaaatacagc gggcacaaaa taaaacgaac ttaaaagacg cggatgcgaa
    19141 tggaaaaata tcatatagaa taggagcat
//
LOCUS       DPUF01000054            6458 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_5257, whole
            genome shotgun sequence.
ACCESSION   DPUF01000054 DPUF01000000
VERSION     DPUF01000054.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_5257"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            236..502
                     /locus_tag="DHV81_01965"
     CDS             236..502
                     /locus_tag="DHV81_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93291.1"
                     /translation="MKYLGRIYYEQKGLINLDDIVDIFIEVGNIHNYLENAALRILLF
                     GNPELNFYQCRLLVLKYGESLSQDDLHGRMVFMRMIQLLEANTV"
     gene            673..1728
                     /locus_tag="DHV81_01970"
     CDS             673..1728
                     /locus_tag="DHV81_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCI93292.1"
                     /translation="MNIVVLAGGLSTERDVSFKTGSMVAKALKENGHKVILLDVFMGY
                     SDREEDLTGIFDRADEISVQVSDIPTEAPDLAKVKASRKDQSPCFFGPNVIKMCQMAD
                     IVFMALHGENGENGKIQAAFDLFGVKYTGSDYLSSAIAMNKETSKQFFLANGIPTPKG
                     ISMTRATRQDDITKLDLTLPCVVKPCCGGSSIGVTIVRDAAEFKAALDDAFKWENELV
                     IEEFVQGREFSVGVIEGKALPIIEIAPKEGFYDYKNKYKAGSTVETCPAELPEQVTKD
                     MQHYAEEVARVIGLDTYSRSDFLLNDKNEMFCLEANTLPGMTPTSLLPQEAAVIGMSF
                     NQLCEHLIDISLKKYEV"
     assembly_gap    1786..1886
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1887..3146
                     /locus_tag="DHV81_01975"
     CDS             <1887..3146
                     /locus_tag="DHV81_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HCI93293.1"
                     /translation="GYLFIPIKGERVDGHKFIPTVFEQGAAIVLSEHELTDPAGPYVL
                     VESTTDAMKKLAAFYRKSLDIKVVGITGSVGKTSTKEMISSVLEQKYSVHKTAGNFNN
                     EIGLPLTIFGIRESHQVAVLEMGISDFGEMHRLSTMSCPDVMVITNIGYCHLEFLGDR
                     DGVLKAKTECFEHMMPDAVAVLNADDDKLATVQTVNGKPAIYYGKESASDVHKSVYTT
                     NIENLGFDGMKAHFVTPEGEFDAQIHIPGEHNVYNAMAAAAVGLQLGLTIAEIKAGIE
                     KAETIAGRTNFIKTHGMTVIDDCYNANPVSMKSSIEVLSHAKGRTIAVLGDMGELGSD
                     EKKLHHEVGEAVGENHIHTLFAAGELAKEYAAGAAEKSSDVDIHYFEDRETMTQELLS
                     YVKEGDTILVKASHFMEFPKVVEALSE"
     gene            3252..>3412
                     /locus_tag="DHV81_01980"
     CDS             3252..>3412
                     /locus_tag="DHV81_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCI93294.1"
                     /translation="MAQNPIVTITMENGDVIKAELYPEIAPNTVNNFISLINHNFYDG
                     VCFHRVIKG"
     assembly_gap    3413..3566
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3567..3775
                     /locus_tag="DHV81_01985"
     CDS             <3567..3775
                     /locus_tag="DHV81_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCI93295.1"
                     /translation="SQFFIMHQDAPHLDGEYAAFGKVIEGMDVVNKIAEVRTDYMDKP
                     LEAQVMKTVTVETFGVDYPEPETV"
     gene            3775..4734
                     /locus_tag="DHV81_01990"
     CDS             3775..4734
                     /locus_tag="DHV81_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCI93296.1"
                     /translation="MIIIIDEASAKLASFYYHDEILKLQWKRAANMSFASDELHDEFQ
                     EKLQEKLPDELREKLYVALPAGESINDFQKTTIQEYADNTAKANMGNIRIPKAPADYI
                     WIVSDRQQKQIADSLGIASIGEPQCGTRYAVESLAELDIEYLERVRRRYNHIPWDIGE
                     TDRCLIRELSLSDLPELYELYDKPGMTDFVEPLYDYETELEYQKAYIENMYGFYEYGM
                     WLVFSRETGKLIGRAGLEHDELGYMIAPELWNQGYATEVCRFIIDYARENTDFEELYC
                     RIDEKNTASVRLAKKLGFTDSGHVDDDINASIYRKNIKNNEKH"
     gene            4794..>5590
                     /locus_tag="DHV81_01995"
     CDS             4794..>5590
                     /locus_tag="DHV81_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCI93297.1"
                     /translation="MKQYAYGVDIGGTTVKIGFFETTGKLVDTWEIPTRTENSGELIL
                     PDIAASIKENNEKHGIEMGDIEGIGMGVPGPIKDDGTVLKCVNLGWGVFNVAQSLSVL
                     CGGIKVKAGNDANVAALGEMWQGGGKGHQDVVMITLGTGVGGGIIREGRIIAGVNGAG
                     GEIGHMPMVDDESECCGCGKKGCLEQYASANGLVRVAERYIAAHRDVATKLDLNAGFT
                     AKDVCDAAKAGDAAGIAAVEQSMKLLGKAMAAVACVMDPEVFVVGGGL"
     assembly_gap    5591..5697
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5833..>6458
                     /locus_tag="DHV81_02000"
     CDS             5833..>6458
                     /locus_tag="DHV81_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCI93298.1"
                     /translation="MADYSTEELEKIADKFRSDTSGIRGTKDFTSPEELYDELKKEIK
                     KYHPSDDTSLVDKAYRVAYDAHKGQARKSGEPYIIHPLCVAIILAELELDKETIAAGL
                     LHDVLEDTIMTMDEMRAEFGDDVAHLVDGVTKLKHLHLTDSTKDPKDKNADRLEMQAE
                     NLRKMFLAMAKDIRVILIKLADRLHNMRTLKYQSKEAQQRIARETQDI"
BASE COUNT         1926 a         1200 c         1592 g         1378 t
ORIGIN      
        1 aaattaaacc caacaccatg tcatattttg tcgaaaactg ctaatcacca atcaatattg
       61 aagcttctaa tagaaatctt caaaatacaa gcgcgtaacg cgcctagaat attgatatat
      121 tatatggaaa aatgaatcca ccaacaaaaa caaagaggga aatttcgcaa agacgacaaa
      181 atttcaaaag aaagacaaaa aaagaagttc tacgaaaaat aaagtcgatg gagggatgaa
      241 atatttggga agaatttatt atgaacagaa aggacttatt aacctcgatg atatcgtaga
      301 tatatttatt gaggtgggaa atatacacaa ttacctggaa aatgcagcct tgcggatttt
      361 gctttttggc aatcctgagc tgaattttta tcagtgccga ttattagtat tgaagtacgg
      421 agaatcactt tcacaggatg atttgcacgg gcggatggta tttatgagaa tgatacagct
      481 gctggaagcc aacacagttt aaaataaaat gagaatttct tttttgaccg ggcaaccggt
      541 ctttcttttt tgcacaaatg gtgttataat tttgaagaaa ttatgttgat tactcatagc
      601 ttaaaaaaga agcactgcat tgtgtttaat taaccaaagc atgataattc aatcaaagaa
      661 aagaggacaa aaatgaacat cgtagtactt gccggagggc tcagcacaga gagagatgtt
      721 tccttcaaga caggaagcat ggtagctaag gcgctcaaag agaacggcca caaagttata
      781 ttattggatg tatttatggg atatagtgac agggaggagg acctgacagg tatatttgac
      841 cgcgcggacg agataagcgt gcaggtcagt gatattccga cagaggcacc tgacctcgca
      901 aaggtaaagg cttccagaaa ggaccagtca ccttgcttct tcggaccaaa tgtcatcaaa
      961 atgtgtcaga tggcggacat tgttttcatg gcactccacg gagaaaacgg agagaacgga
     1021 aagatacagg cagcatttga tcttttcggt gtcaaatata caggcagcga ttatttaagc
     1081 tcagccatcg ccatgaacaa ggagacatca aagcagttct tccttgcaaa cggtatccct
     1141 actccaaagg gtatttccat gacaagagcg accagacagg acgatatcac aaagcttgac
     1201 ctgacacttc catgcgtggt aaagccatgc tgcggcggtt caagcatcgg tgtcaccatc
     1261 gttagggatg cggcagagtt caaggcggca cttgacgatg ccttcaagtg ggaaaatgag
     1321 ctggttatcg aggaatttgt acagggcagg gagttctctg tcggcgttat cgagggcaag
     1381 gctcttccta ttatagagat tgcaccgaag gagggcttct acgattataa gaataaatac
     1441 aaggcgggca gtacagtaga aacatgtccg gccgagcttc ctgagcaggt gacaaaggat
     1501 atgcagcact atgcagagga ggttgcgcgt gttatcgggc ttgacacata ttcacgctca
     1561 gactttttgc tcaatgacaa aaatgagatg ttctgcctcg aggcaaacac actgccgggt
     1621 atgactccga ccagtcttct gccacaggag gcagcggtta tcggtatgag ctttaatcag
     1681 ctctgtgagc atctgattga catatctttg aaaaaatatg aggtgtagca tgtcggataa
     1741 gtacatgaag aatcttacat ttgcaaatat cgcaaaggcc tgcgannnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnggct atctttttat cccgataaag ggagaaaggg
     1921 tggacggaca caaattcatt ccgactgtgt ttgagcaggg ggcggcaatt gtgctctctg
     1981 agcatgagct gacggatccg gcaggcccat atgtgcttgt tgaatcaacc accgatgcca
     2041 tgaaaaagct tgcggccttt tacagaaaaa gccttgatat caaggtggtg ggcatcaccg
     2101 gaagtgtcgg caagaccagc accaaggaga tgatatcatc agtccttgag cagaaataca
     2161 gcgtccacaa gaccgcaggc aatttcaaca acgagattgg cctgccactc accattttcg
     2221 gaatcaggga aagccatcag gtagcggtgc ttgagatggg catatctgat tttggcgaga
     2281 tgcacagact ttctactatg tcttgcccgg atgttatggt catcaccaat ataggctact
     2341 gccatctgga gtttttgggt gacagggatg gtgtgcttaa agcaaagaca gagtgctttg
     2401 agcatatgat gccggatgcg gtcgcagtgc tgaatgcaga tgacgacaag ctggccacag
     2461 tgcagacggt aaatggaaag cctgccatat actatggcaa ggaatcagct tcggacgttc
     2521 ataaatcagt atataccaca aatattgaaa acctgggctt tgacggcatg aaggctcatt
     2581 ttgtcactcc ggagggtgag tttgatgcac aaatccatat cccgggtgag cacaatgtat
     2641 acaatgccat ggcggcagcg gctgtcggat tgcagcttgg cctcacaata gcagagataa
     2701 aggcaggaat cgagaaagca gagacaatcg caggtcgtac caatttcatc aaaacacacg
     2761 gcatgacagt gatagatgac tgctacaatg caaatcctgt atccatgaaa tcctctatcg
     2821 aggtgctttc acatgcaaag ggcaggacaa tagcagtgct tggagacatg ggagagcttg
     2881 gaagcgatga gaaaaagctg caccatgagg taggcgaagc agttggagag aatcatatcc
     2941 atacactttt tgccgcagga gagctggcaa aggaatatgc agccggagcc gcagagaaat
     3001 cgtccgatgt agacatccac tatttcgagg atagggagac tatgacacag gagcttcttt
     3061 catatgtgaa ggagggcgac accatactgg tgaaggcatc acacttcatg gagtttccga
     3121 aggtagtcga ggctctgagt gagtaaaatt aggtaaaatc aatcgggtag aaattaattc
     3181 gatatttaat aattgcaagg aatggatatt ctccaagcct cataatatta taaaagaaag
     3241 agagaaaaat aatggcacag aatccaatcg taacaatcac aatggaaaac ggcgatgtaa
     3301 tcaaggcgga gctttatccg gaaatcgcac caaacacagt aaacaacttt atcagcttaa
     3361 tcaatcacaa cttctatgac ggagtatgct tccaccgtgt aatcaagggc ttnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnngcag ccagttcttc atcatgcatc aggatgcacc
     3601 acatctcgac ggcgagtatg cggcattcgg caaggtaatc gagggcatgg atgtggtaaa
     3661 caagatcgca gaggtgcgca cagactacat ggacaaacca cttgaggctc aggtcatgaa
     3721 gacagttaca gttgagacct tcggagtgga ctatccggag ccggagacag tttaatgata
     3781 ataattattg atgaggcaag cgcaaaactt gcctcttttt actatcatga cgagatatta
     3841 aagctgcagt ggaagcgcgc agccaatatg tcatttgcgt cagacgagtt gcatgatgaa
     3901 tttcaggaaa aactgcaaga aaaactacct gatgagctgc gggaaaaact gtatgttgcg
     3961 ctaccggctg gtgaatcaat taatgacttc caaaaaacaa cgatacaaga atatgcagat
     4021 aacaccgcga aagctaatat gggaaatata agaataccga aagctccagc agactatatc
     4081 tggatagtat ccgacaggca gcaaaagcaa atagccgact cactcggcat agcatcaata
     4141 ggagaaccgc agtgcggcac gcgctatgca gtggaaagcc ttgcggagct tgatattgag
     4201 tatctggagc gggtgcgcag gcggtataat cacatcccgt gggacatcgg cgagacagac
     4261 aggtgtctga tacgtgagct gtccttgtca gaccttcctg agctctatga gctctatgac
     4321 aagcccggca tgacagactt tgtggagcca ctttacgact atgagacaga gcttgaatac
     4381 cagaaagcgt atatagagaa catgtatgga ttttacgagt atggcatgtg gcttgtattt
     4441 tccagggaaa cagggaagct gataggcagg gcagggcttg agcacgatga gctgggctat
     4501 atgatagcgc cggaattatg gaaccaaggc tatgccacgg aggtgtgccg cttcatcata
     4561 gactacgcgc gcgaaaacac agactttgaa gaactttact gccggataga cgagaaaaat
     4621 acggcatcgg tgcgtcttgc caaaaagctt ggatttacag atagcgggca tgtggatgat
     4681 gatataaacg cttctatata tagaaaaaat ataaaaaaca atgaaaaaca ttgaaaaata
     4741 tgcaattagg caataaaata taaacatagt ttaacgattt ggaggacgaa aaaatgaaac
     4801 aatatgcata cggagtagat ataggtggaa caactgtaaa gataggcttt tttgagacaa
     4861 caggaaagct tgtcgataca tgggagattc ctacacgcac agagaattcc ggtgagctca
     4921 tcctgcctga tatcgcagca tccatcaagg agaataatga gaagcacggc atcgagatgg
     4981 gtgacattga gggtataggc atgggagtgc cgggacctat aaaggatgat ggaacagtat
     5041 tgaagtgtgt caacctcggc tggggtgtgt tcaatgtggc acagtcactc tcagtactgt
     5101 gcggtggcat caaggtaaag gccggaaacg atgccaatgt agctgcactc ggtgagatgt
     5161 ggcagggtgg cggaaagggc catcaggatg tagtcatgat cacactcggc acaggcgtag
     5221 gcggaggaat catccgcgag ggcagaatta tagccggagt aaacggagca ggcggagaga
     5281 taggacatat gcctatggta gatgatgagt cagagtgctg tggctgcggc aagaagggat
     5341 gcttagagca gtatgcatca gcaaacggtc tcgtaagagt ggcagagaga tacattgcag
     5401 cacacaggga tgtggcaaca aagcttgatt taaatgcagg cttcacagcc aaggatgtat
     5461 gcgatgcggc aaaggcagga gatgcagcgg gcattgcggc agtagagcag tctatgaagc
     5521 ttctcggcaa ggcaatggca gcagtagcat gcgttatgga ccctgaggta tttgttgtag
     5581 gcggaggcct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag
     5701 cttgccacac tcggcaactc agccggcatg tatggcggag taaagatggt tatcggctaa
     5761 cattggattg caatttttaa agaaataaaa tataatggct actaaagata cactacaatc
     5821 gaggtgagac tattggcaga ttactcaaca gaagaattag aaaaaatagc ggacaaattt
     5881 cgctcagata ccagcggtat aaggggaaca aaggatttta cgagcccaga ggagctttac
     5941 gatgagctta aaaaggaaat caaaaagtat catccgtcgg atgacacaag ccttgtggac
     6001 aaagcctaca gggtagctta tgatgcccat aaggggcagg cgcgtaagtc aggagagcct
     6061 tatatcatac atccgctgtg tgttgccata atactcgccg agctcgagct ggataaggaa
     6121 accattgcag caggactgct ccatgatgtg ctggaggata ccatcatgac tatggatgag
     6181 atgcgcgcgg agtttggaga cgatgtcgca catctggtgg atggtgtcac gaagttaaag
     6241 cacctgcatc tgacagacag caccaaggat ccgaaggaca agaacgcgga caggcttgag
     6301 atgcaggctg agaatctgcg caagatgttt ctcgccatgg caaaggatat cagagtcatc
     6361 ctcataaagc tggcagacag actgcacaat atgcgtacac tcaagtacca gtccaaggag
     6421 gcacagcagc gtatcgccag agagacacag gatatcta
//
LOCUS       DPUF01000055            2746 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16703, whole
            genome shotgun sequence.
ACCESSION   DPUF01000055 DPUF01000000
VERSION     DPUF01000055.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16703"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            104..1909
                     /locus_tag="DHV81_02005"
     CDS             104..1909
                     /locus_tag="DHV81_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCI93299.1"
                     /translation="MVLAVEIIVCCLIFGIYRVIRIKRDPAYKISNMPEKLQKKVMHM
                     RGYRNRNIRIMTDWEKFVKKLPTLIFWTIALVILTSIAGATSFSTGFVFALLIWMAVL
                     LFLELVVYCGWYAHTPKVWIKGTEDMAKKTYTNYAHYIGLIPQRALMGIVVAIIVGLV
                     IDMIPRLDNNNYSPKYTEIEDTLKAACDNYRIPGMAVEVVDAEGVLFSGTYGECKSLD
                     TPFITGSLSKSFTAACIMKLYEGGHLNIDSPVNPYLDAAEVFKNPKDATRITIRQLLN
                     HTSGLGVYQHVGNAKIVGKNGEYTYANVNYDILGLIVEKVSGVSYSDYLTATFFTPLG
                     MTHSSAAYAKAKKDGLITGHNNYFGFSVESDVKYPLSDSWSTVPAGYIASSANDMGKY
                     LQMYLRGGYGILSDKSLSTMFRATVPMDESGDTGYGMGWVRSDKYVETVYNHTGLTEN
                     YIADMYLLPESGVGVVFLANTNDYMVTNNLMNKVTSKVVMTLMGYATDELDPKDYVDA
                     HLFYDLVFAAFILVALMEIIKSHKWRTDNSGNLIANIFLHLFLPVAIVIAPIVGGIPY
                     WVIKDYVPDLFIVSCLSVVLLAIGGILKLKNRRNS"
     gene            complement(1938..2582)
                     /locus_tag="DHV81_02010"
     CDS             complement(1938..2582)
                     /locus_tag="DHV81_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93300.1"
                     /translation="MEQKTPFKELTFSQKIQYIWDYYKIPIFLVIIGIIAVTTFIHGR
                     LAQKNTVLSVLCVNVENSTPEDSGADIFNDFLTDNGYDLSKDEIALNKNICVDVDKSG
                     LDYQNMAVLTAYFASNEYDICFMDDKTFDYYAKNVCFEDISKYLDKAVLEKYKDRLAY
                     VTIDGKEYPCGIRLSADDNEFVKASGLYSSCTVGVCCDSSHDAMVKKLCGYILQ"
BASE COUNT          824 a          493 c          626 g          803 t
ORIGIN      
        1 tattcatatt attttgtgtt tacatagcac aaaaaatgtg ctaaattttt atgtaaagta
       61 cagtctcaaa tgactgtaaa taagacataa ggtggttgat agaatggtac tggcagttga
      121 gataatagtc tgctgcttaa tatttggcat atacagagta atacggataa agcgggatcc
      181 ggcatataag ataagcaata tgcccgagaa gctgcagaaa aaggtcatgc atatgcgcgg
      241 ctacagaaat cggaatatcc gtatcatgac agactgggag aagtttgtaa agaagcttcc
      301 gacacttata ttctggacga tagcgcttgt catactgaca tcaatagcag gggcaacaag
      361 cttttcaacg ggctttgtgt ttgcactttt gatatggatg gcagtcctat tgtttcttga
      421 actggtagta tactgtggct ggtatgcaca tacaccgaag gtgtggataa agggtaccga
      481 ggatatggcg aaaaagacat ataccaatta tgcacattat atcgggctga taccacagag
      541 agctttgatg ggaatagtgg ttgctattat agtgggtctt gtgatagata tgattccaag
      601 gcttgacaac aacaattatt cgccgaaata tacagagata gaggatacct taaaggcagc
      661 ctgtgacaat tacaggattc cgggcatggc tgttgaggtg gtagatgcag agggcgtgct
      721 tttctccggg acttatgggg agtgcaagag ccttgacaca ccatttatca ccggttcact
      781 cagcaagtcg tttactgcgg catgcatcat gaagttgtat gagggaggac atctgaatat
      841 tgacagcccg gtaaatcctt atctggatgc agctgaggta ttcaaaaatc caaaggatgc
      901 gacaaggatt acaataagac agctgctcaa tcataccagt ggacttggcg tgtaccagca
      961 tgtgggaaat gcaaagattg ttggcaaaaa tggagaatat acctatgcga atgtcaatta
     1021 tgacatactt ggtcttatag tggaaaaagt atcaggtgtt agctacagcg attatctgac
     1081 agcgacattt tttacaccac ttggcatgac tcacagcagc gcagcctatg caaaagcgaa
     1141 gaaggatggt ctgattacag gccacaacaa ttatttcggt ttttcggtgg aaagcgatgt
     1201 gaagtatccg ctcagcgatt cgtggtccac tgtaccggcg ggctatattg catccagcgc
     1261 aaatgatatg ggaaaatatc tgcagatgta tctgcgtggc ggctatggaa tactctctga
     1321 caagagcctt agcacaatgt ttcgggcaac agttcctatg gatgagtcgg gtgatacagg
     1381 ctatggtatg ggctgggtac gttcggataa atatgtcgag acagtgtaca atcacacagg
     1441 tctcactgag aactatatcg cagacatgta tctgcttccg gagagcggtg taggtgttgt
     1501 atttcttgca aataccaatg actatatggt cacaaacaat ctgatgaaca aggtaacctc
     1561 aaaggttgtg atgacgctta tgggatatgc aacagacgag cttgacccaa aggattatgt
     1621 cgatgcgcac ctgttctatg accttgtttt tgcagcattt atattggttg cgctgatgga
     1681 gattatcaag tcgcacaaat ggagaacaga caatagcgga aacctgatag cgaatatttt
     1741 tctgcatctg tttttgccgg tggccatagt tattgcgcct attgtcggag gcataccata
     1801 ctgggtaata aaggattatg ttcctgattt gtttattgtt tcatgcctga gtgtagtact
     1861 tcttgcaata ggtggaatcc tgaagcttaa aaacaggaga aattcataag ataaaagcag
     1921 aagaaataga aatgcggcta ctgtaatatg tagccgcata attttttgac catagcatcg
     1981 tgggaggagt cacagcacac tccgacagta catgaagagt ataagccgga ggctttgaca
     2041 aattcattgt catctgctga aagccttata ccacaaggat attccttacc atctattgtc
     2101 acatatgcaa gcctgtcctt atatttttca aggacggctt tatccagata tttgcttata
     2161 tcttcaaagc agacattttt ggcgtaataa tcaaaggtct tatcatccat aaagcatata
     2221 tcgtattcgt tagaagcaaa gtatgctgtg aggactgcca tgttctgata gtctaggccg
     2281 cttttgtcta catcgacaca gatatttttg tttaaggcaa tttcatcctt tgacaaatca
     2341 tagccgttat ctgtgagaaa gtcattgaaa atatcggcgc cgctgtcctc tggggtgctg
     2401 ttttctacat ttacgcataa tacgctcaaa acagtatttt tctgcgcaag ccgaccatga
     2461 ataaaggttg tgactgctat gattccgata atgacaagaa atatcggtat tttgtaatag
     2521 tcccatatat attgtatttt ctgactgaat gtcagctcct taaaaggtgt cttttgttcc
     2581 atgattttat ctccctctga tatgatgtgc tgtagtttaa aaatattata aggtaattta
     2641 tagaaattta ccaataaaca ttctattaac tgcctttttc tttttagatt attatgctat
     2701 aatgagccaa aacacagata atacaaaagg agaataatat gaaagc
//
LOCUS       DPUF01000056            3867 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_35291, whole
            genome shotgun sequence.
ACCESSION   DPUF01000056 DPUF01000000
VERSION     DPUF01000056.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_35291"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="DHV81_02015"
     CDS             complement(<1..749)
                     /locus_tag="DHV81_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93301.1"
                     /translation="MQAYKDLVKALPGLKENMPRAFYMLAELFDYGSFDICRSDDKYI
                     IPYIMNDAVECYLTVENAVLKGDYQSEEEIISASLVLGSEKGYGLILHQQDNVITLWF
                     DNLHVHEACFKYHEIGHFWVKGQEQWRMLVYMVGTIADKYMYMGKEYCNETECFIQSL
                     IYFAPFRRWTPVPGDLMEYHFPARVEGIDIMEELCREVSDIDYLKLIARYRANPCEKT
                     EKLLSRHLADAKRVPLYQYIYKLVIKASKDYP"
     gene            complement(830..912)
                     /locus_tag="DHV81_02020"
     tRNA            complement(830..912)
                     /locus_tag="DHV81_02020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(876..878),aa:Leu,seq:tag)
     gene            complement(1062..2009)
                     /locus_tag="DHV81_02025"
     CDS             complement(1062..2009)
                     /locus_tag="DHV81_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI93302.1"
                     /translation="MIWKILTVILAVSVAVLIAVLVKIRAQLRDINEQLDFLCEKDTN
                     MLLLTDTNMADIGRLKERINRFLEQWHRQREAAAKKEQMISDTYTNLSHDIRTPLTSL
                     DGYFQLLRDETDKSAQEHYIDIIQERITSLKDMLEELFMFTKLKNDSFKLELSNCCVS
                     RLLKQTVFSYYEEWKKQGIEPSLDICEDTIFITANVQALRRVFQNVIKNALVHGQKSI
                     CIQMKQQDSCNCRASDDERTSKNRIVHILVSNDVKNPDDIDVDKVFERFYKADEARSI
                     SSSGLGLSIAKELVERMGGSIEARLEGKRFVVEIQFECE"
     gene            complement(2034..2936)
                     /locus_tag="DHV81_02030"
     CDS             complement(2034..2936)
                     /locus_tag="DHV81_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI93303.1"
                     /translation="MFNAVRMDIYRLIHTKSAYVILVIAMALAIAMSGMTALVRSMAT
                     ESIVETTDDVTMVSESDSEIADEYDTASAESSNGPTATVGLEMDESDMSDEVPTIADM
                     VESDIAGLDLALLLAIFTVLFSTADLNSGYIKSVGGQVKCRGVLLFSKMIALSVFTLV
                     AMALDVIVQCIATPLFLHGAEFGDAADLFKMLGSQYVVTLLFVYFVMAMAIIIKNNVI
                     SMIIAVCMCTGIFTLIFSGINILIEKIGAKNFDINDYLIVNQISELDLSASVKTVGGA
                     FAVAAVWAVVSLIAVYGVFKRRDI"
     gene            complement(2929..3852)
                     /locus_tag="DHV81_02035"
     CDS             complement(2929..3852)
                     /locus_tag="DHV81_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin ABC transporter ATP-binding protein"
                     /protein_id="HCI93304.1"
                     /translation="MIVSEILLQTKALTKQYGHQKAVDNVDIHIKKGAIYGFIGRNGA
                     GKTTCMRMIGGLAKPTSGEISMFGYAGKDLSKVRSRVGCLIEAPGVYPNMTAKENIEM
                     KCRLFGISKKGYAEGILDKVGLLNVGKKKTKNFSLGMKQRLGIGMALVGEPDLLVLDE
                     PINGLDPQGIAEVRDTIQNLCAQQDMTVFISSHILEELSKLATDYGIINNGALLQEIT
                     REELLSKCSEKITLTVDNPNKAVPVLDKMGFVHYMIVDKNHIDVYERLNYSAALNTAL
                     VTAGVSVAQLAVTGMELETYFLSITGGATNV"
BASE COUNT         1042 a          999 c          664 g         1162 t
ORIGIN      
        1 ggataatcct ttgatgcctt tatcacaagc ttataaatat actgataaag cggtactctt
       61 tttgcatcgg ctaaatgtct gctcaaaagc ttttccgttt tctcacatgg atttgccctg
      121 tagcgtgcaa tgagcttaag ataatcaatg tccgaaacct ccctgcaaag ctcctccata
      181 atgtctattc cctctacacg ggccggaaaa tgatactcca tcaaatcgcc aggtaccggt
      241 gtccatctgc gaaacggtgc aaagtaaata aggctctgta taaagcattc tgtctcgttg
      301 cagtattcct ttcccatgta catatactta tcagcgattg ttcctaccat atatacaagc
      361 atgcgccact gctcctgtcc ctttacccag aaatgtccta tttcatggta tttaaagcat
      421 gcctcatgca catgcaaatt atcaaaccag agtgtgatca cattgtcctg ttggtgcaga
      481 atgagtccgt agcctttttc acttcctaaa accaacgatg ccgaaatgat ttcttcctca
      541 ctctgataat cgccctttaa cactgcattt tccacagtca gatagcattc caccgcgtca
      601 ttcatgatat atgggattat atatttgtca tcacttctgc atatatcaaa tgaaccgtaa
      661 tcaaaaagct ctgcgagcat gtaaaatgcg cgtggcatat tctcttttaa gccaggaagt
      721 gcttttacta aatccttgta agcctgcatt gtggtatcct ttcgtatagg aaaaatgata
      781 aaaaaataag aatttcagaa gtaataaaaa cctctgaaac ccttgtaaat agtacgggca
      841 acaggacttg aacctgcacg gtctcccact agaacctaaa tctagcgcgt ctgccaattc
      901 cgccatgccc gcatatatat gccgcgtaca gtagcgcatc tgcctacaat tgaacgttag
      961 cagttcaatt gtcaagaatt ccgccatgcc cgcaaacact ttgacagtat atcattttgt
     1021 ttcatgtgtg tcaatattat ttatcacatt acaaaagtga actattcaca ttcaaactgt
     1081 atttccacaa caaaccgctt cccctcaagc cttgcttcaa tactcccgcc cattctctcc
     1141 acaagctcct ttgcaatcga aagaccaagc ccgctcgatg atatgctcct agcctcatcg
     1201 gctttgtaaa aacgctcaaa caccttgtcc acgtctatat catcaggatt ttttacatca
     1261 tttgaaacca aaatatggac aatcctattt tttgaagtac gctcgtcatc tgaagccctg
     1321 caattgcacg aatcctgttg cttcatctgt atgcagatgc tcttctgccc atgcaccagc
     1381 gcatttttta ttacattctg aaacacccgc ctgagcgcct gcacatttgc agtgataaat
     1441 atcgtatcct cacatatatc aagtgacggc tcaatgccct gctttttcca ctcctcataa
     1501 tacgaaaaca ctgtctgctt taaaagtctg ctgacacagc aattagacag ctctagcttg
     1561 aagctgtcat ttttaagctt tgtgaacatg aaaagctcct cgagcatatc cttgaggctt
     1621 gtgatgcgct cctggatgat atctatataa tgctcctgcg cgctcttgtc agtctcatcc
     1681 ctaagcagct gaaaatagcc gtccagcgag gtaagcggag tgcggatatc gtgcgacagg
     1741 tttgtgtacg tgtcagatat catctgctcc tttttcgctg ctgcctcacg ctgcctgtgc
     1801 cattgctcta aaaatctgtt tatacgctcc ttaagccttc cgatatcagc catgttggta
     1861 tcggttaaaa ggagcatatt tgtatccttt tcgcataaaa aatcaagctg ttcattaatg
     1921 tcacgaagct gtgcccttat ttttacgagc acagctatta agacagctac cgaaactgca
     1981 agtattacag tcagtatttt ccaaatcatg ttttattcct cattcaaaac acattatata
     2041 tccctgcgtt taaacacacc gtacactgca atcagtgaca ccactgccca cacagcggct
     2101 accgcaaatg caccacctac ggtctttaca gatgcactca gatcaagctc agaaatctga
     2161 ttaacaataa ggtaatcatt tatatcaaag ttctttgctc cgattttttc aataagtata
     2221 ttgattccac taaaaatcag tgtaaagata cctgtacaca tgcacacagc gataatcatg
     2281 ctgattacat tgttttttat aatgattgcc attgccatga caaaatatac aaataaaagt
     2341 gtgaccacat actggctgcc aagcatctta aataagtctg ctgcatctcc gaattctgct
     2401 ccatgtaaaa acagtggagt cgcaatacac tgtacaatca catcaagtgc catagccaca
     2461 agtgtaaata ccgaaagcgc tatcatcttt gaaaacagca gcactccacg gcacttcacc
     2521 tgtccgccta cgctttttat atagccgctg tttaggtctg ctgttgagaa gagcaccgta
     2581 aatattgcaa gtaaaagtgc caggtcaaga cctgctatat cgctttcaac catatcggct
     2641 atggtcggca cctcatctga catgtcggat tcatccattt caagcccgac tgttgccgtt
     2701 ggtccatttg acgattccgc tgatgctgta tcgtattcat ctgctatctc actgtctgac
     2761 tcacttacca tagtgacatc atcagttgtt tccacgatgc tctctgtcgc catgcttctg
     2821 acaagagctg tcataccgct catagctatt gcgagtgcca tagctattac aagtataaca
     2881 taagctgact ttgtatgtat cagtctgtaa atatccattc ttaccgcatt aaacatttgt
     2941 cgcacctcct gtgatactaa ggaaatatgt ctcaagctcc attcctgtga ccgccagctg
     3001 tgccaccgaa actcctgctg tgaccagcgc tgtatttaag gcggcgctat aattcaggcg
     3061 ctcatagaca tctatatgat ttttgtccac tatcatgtag tgcacaaagc ccatcttatc
     3121 gagcacaggc actgctttat ttggattgtc cactgtcagt gtaatcttct cactgcactt
     3181 ggaaagcagc tcctccctgg taatttcctg aagcagggct ccattattta taatgccgta
     3241 atctgtggca agctttgaaa gctcctccaa tatatggctt gaaataaaca cagtcatatc
     3301 ctgctgtgca cacagattct gaatcgtatc acgcacctct gcaattccct gtggatcaag
     3361 tccgttgata ggctcatcaa gcactaaaag gtcaggctct ccaaccagcg ccatgccgat
     3421 tccaagcctc tgcttcatac caagtgaaaa attctttgtc tttttctttc ccacatttaa
     3481 cagacctacc ttatcaagta tgccctctgc atagcccttt ttactgatgc cgaagagacg
     3541 gcacttcatc tcgatatttt ccttcgccgt catgtttgga tatacacccg gtgcctcaat
     3601 caggcagccc actcttgagc gtacctttga caaatccttt ccagcgtatc caaacatgga
     3661 tatttctccg cttgtcggct ttgccagacc gccgatcatt ctcatacagg tagtcttgcc
     3721 ggcaccgttt ctgccgataa atccatagat tgcacccttt tttatgtgga tatccacatt
     3781 gtcaactgcc ttctggtgcc catactgctt ggtcagtgcc tttgtctgca ataatatttc
     3841 actcacaatc aaatctcctt ttctttt
//
LOCUS       DPUF01000057            2902 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_49446, whole
            genome shotgun sequence.
ACCESSION   DPUF01000057 DPUF01000000
VERSION     DPUF01000057.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_49446"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(172..2250)
                     /locus_tag="DHV81_02040"
     CDS             complement(172..2250)
                     /locus_tag="DHV81_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCI93305.1"
                     /translation="MGYDEKIFKAKANIKARRLWLVFAILLTANYGTDTANGAYSVSN
                     YIIFVILCWLPFVCGDILLKRKGKDNDHYRLAFVIGYGIFYVFLLCTTTSPIAFTYIL
                     PIISLIVIFKDEKFMIYCAVANMLSLIASIAFHIFVLGQNTAIDHKNFQLQIACLLLC
                     YIGYIMSVRHLTESDAALTESIKSDLNRVVTTVEQVKTASNTIMDGITVVRELASENK
                     HGSDVVVERMNELTGNNEQLQSHTASSQEMTTDISSQVENVAAMINDMVSLTAESGKH
                     AKVSSEDLEGLAQTAKTMSELSTEVENILTTFRDEFEMVKNETGTIDNISNQTNLLAL
                     NASIEAARAGEAGKGFAVVAEQIRTLSTETRNSSGQISEALSRLDEISGKMTSSIEET
                     LRLIQLTLEKVMQTGENVEKITKDSNKLGSHIHEIDAAMQEVEASNQQLVDNMEKVSE
                     IVETMTSCIDASDAISRKMLSKYDESATNINNIETVIQSLMHELGVGGFMGLDDIRPG
                     MKAKVILTDVQTGNEFHCEVKAVDENGLKLVSGGLSVDSSRPCNLCVTVGNVMYCWKD
                     LTITDGLMITVNTQPEILNRRKYPRMDLSNNCTIKLKGTDTTFKGTLDNISANGFAFL
                     TKDPYFVDHKGTKVTISIEDFALADHSVLEGYVIRCSNNEGTYIVGCQMPEDNYYIQT
                     YVNEQLRAHS"
     gene            2446..>2902
                     /locus_tag="DHV81_02045"
     CDS             2446..>2902
                     /locus_tag="DHV81_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H dehydrogenase"
                     /protein_id="HCI93306.1"
                     /translation="MNILLINGSPKGKRSNSLRLANSFIEGFKEGYKSKNEAVSIDEM
                     HVASMNVGACKGCFACWQKTPGVCCINDDMQTVIGKMLEADIVVWSFPLYYFSVPGIL
                     KNVIDRQLPMSLPFMSTKDDGYGSGSHDCRYDMEGKRHVLISTCGFYSAE"
BASE COUNT          842 a          579 c          613 g          868 t
ORIGIN      
        1 ggtatcataa tattcagcat tcacagctac aatgtatata tgagagtcca gagaggaaaa
       61 acatcatgca gatagaagat aaagataata gatagaagat aataggatat agaagattat
      121 caatcaaaaa agctatgaga gcccggttaa aggctttcat agcttttatt tttatgagtg
      181 agcacgaagc tgttcattga catatgtctg gatataataa ttatcctcag gcatctggca
      241 gccgacgata tatgttccct cattatttga gcagcggatt acatatccct ccaatactga
      301 gtggtcagca agcgcaaagt cctctatcga gatagtaacc ttcgttcctt tatgatccac
      361 gaaataagga tcctttgtca aaaatgcaaa gccgtttgca cttatattgt caagtgtacc
      421 cttgaatgtg gtgtcggttc ctttaagctt gatagtgcaa ttgtttgata aatccatgcg
      481 aggatatttc ctacggttca agatttcagg ctgcgtattg actgttatca taaggccatc
      541 tgtgattgtg agatccttcc agcagtacat tacattgcca actgtaacac acagattgca
      601 aggtctggat gagtctacag ataagccgcc tgatacaagc ttaagaccgt tttcgtcaac
      661 agcctttact tcgcaatgga attcgttgcc ggtctgtaca tctgtcagga tcactttggc
      721 cttcataccc gggcggatat catcaagtcc cataaatcca ccaactccga gctcgtgcat
      781 gagagattgg ataacagtct cgatattatt gatatttgtt gcgctttcat catatttact
      841 gagcatcttg cggcttatcg cgtcagatgc atcgatacat gaggtcatgg tttctacgat
      901 ttcagatacc ttttccatat tgtcgacaag ctgctggtta gatgcttcaa cctcctgcat
      961 tgcggcatcg atttcatgga tatggcttcc gagcttattg gaatcctttg tgatcttctc
     1021 tacattttca ccggtctgca ttaccttttc aagagtgagc tggatgaggc ggagcgtttc
     1081 ttcaatggat gaggtcatct ttccggaaat ctcgtcaagc cgtgagagag cctcactgat
     1141 ctgaccggat gaatttctcg tctcagtgct taaagtacgg atctgttcag caacaacagc
     1201 aaagccctta cctgcttcac ctgcacgtgc agcctcgata gaagcgttaa gagcgaggag
     1261 gtttgtctgg tttgaaatat tatcaattgt tccggtttca tttttaacca tctcaaattc
     1321 atctctgaat gtagtcagga tgttttcaac ctctgtagaa agctccgaca tagtttttgc
     1381 tgtctgggca agaccttcaa ggtcctcaga gctgaccttg gcatgcttgc ctgattcggc
     1441 tgtcagggaa accatatcgt ttatcatggc tgcaacattt tcaacctgtg agcttatatc
     1501 ggtagtcatt tcctgggaag aagcagtgtg ggactgaagc tgctcattat ttccggtaag
     1561 ctcattcatt ctttcaacga caacatctga gccgtgcttg ttttcagaag caagctcacg
     1621 gaccactgtt ataccgtcca ttatagtgtt gctggcagtt ttaacctgct caactgtcgt
     1681 gacaacgcga ttcaaatcag actttatact ttcggtgagt gcagcatcgg actcagtcag
     1741 gtggcgcacc gacatgatat agcctatgta acaaagcaaa agacatgcta tctgaagctg
     1801 aaagttttta tgatctatag cagtgttctg accaagcaca aatatgtgaa atgcaatact
     1861 tgcaataagg cttagcatat ttgcaacagc gcagtaaatc atgaattttt catccttaaa
     1921 tattacaata aggctgatta tcggaagaat gtaggtgaat gcaatcggtg aggttgtcgt
     1981 acacaaaaga aatacgtaaa aaataccata tccgatgaca aatgcaagcc tgtaatgatc
     2041 attatccttc cccttccttt ttagaagtat gtcaccacac acgaaaggca gccagcataa
     2101 aataacaaaa atgatataat tactgacaga ataagcgcca tttgctgtgt cggtaccata
     2161 atttgcggtc agcaatatag caaaaacaag ccataatctg cgggccttga tattcgcctt
     2221 tgctttaaaa attttttcat cgtaacccat atgaattctc ctgatttgaa ataatataac
     2281 tctattttac tatcatagta ctataattac aaggcatgct taagaatttg aatgtatttt
     2341 gaatgtataa ttatggtatt gtttctaagt ctaaacttta attgtttttt gcctgtcaat
     2401 ccgatatact aaaattaact atatacttac aggagggcat aaagcatgaa catacttctg
     2461 attaacggaa gtccaaaagg caaacgaagc aattcgttaa ggcttgcaaa cagctttata
     2521 gaaggcttca aagaaggata taagagcaaa aatgaagctg tatccattga tgaaatgcat
     2581 gtagcctcaa tgaatgtagg agcgtgtaag ggttgctttg catgctggca gaagacaccg
     2641 ggggtgtgct gcataaatga cgatatgcag actgttattg ggaaaatgct tgaagcggat
     2701 attgttgtgt ggtcgtttcc tctgtactat tttagtgtgc cgggaatact aaagaacgtg
     2761 attgacaggc agcttcccat gagcctgccg tttatgagta caaaggatga cggatacgga
     2821 agcggcagcc atgattgcag atatgacatg gaaggcaaaa ggcatgtgct aatctctacc
     2881 tgtggctttt attctgcaga gg
//
LOCUS       DPUF01000058           11259 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4148, whole
            genome shotgun sequence.
ACCESSION   DPUF01000058 DPUF01000000
VERSION     DPUF01000058.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4148"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="DHV81_02050"
     CDS             <1..150
                     /locus_tag="DHV81_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93307.1"
                     /translation="IDLDAIDPNQMTDREKGYLNAYHARVYELVSPFLNDEEAQWLKK
                     YTRAI"
     gene            220..2931
                     /locus_tag="DHV81_02055"
     CDS             220..2931
                     /locus_tag="DHV81_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCI93308.1"
                     /translation="MSEKLVLIDGHSILNRAYHGLPDLTNSEGLHTNAVYGFLNIMFK
                     ILDEEKPDYLTVAFDVHAPTFRHRMFDAYKGTRKPMDEELRQQVPMIKEMLTAMGIKI
                     VEKEGYEADDILGTLSVRAEKAGMDVAIISGDRDLLQLATDHVMVRIPKTKKTGTEIE
                     NYNTADVIEKYGVTPKEFIDVKALQGDTSDNIPGVPGIGEKTAGALIAKYHCIEAVHE
                     DAENVKPPRASKNIVEYWEQALMSKELATIILDAPVDYEFSDAKLSGGVESLYTEEAF
                     LLCKRYEFKNMLSRFNVEAPKNNAEEHFVVVRDLKTAERVFEKAKDKEVAFAVVPGES
                     LENSESDGQLSLFSEPVSNDYLAVSICFSEEDIYFICTGDEISSSFIDEKLNTLEVKS
                     WISPDLKTNLHRFKSKEIKADDRGRYFDMMVAAYLINPLVGEYPYDAVAKDYLGLMLS
                     SKKDYLGKLDFTRMMKEDEKKAVDCACYEVYTAWKSKPVLLQKLKEMDMLTLYKDIEL
                     PLVFVLYDMENEGIRADGIKLKEYGDKLAVSITELEKKIYEAAGEEFNINSPKQLGVI
                     LFEKLGLPNEKKTKTGYSTAADVLEKLAPEYPIVADILEYRQLTKLKSTYADGLSGYI
                     ASDGKIHTTLNQTITATGRLSSTEPNLQNIPIRIELGKLIRKVFLPEDGDLFVDSDYS
                     QIELRVLAALSGDERMIEAFKNGQDIHRSTASLVFDTPFDEVTDLQRRNAKAVNFGIV
                     YGISAFGLSNDLGISRKEAQGYIDSYFVKYPKIKEFLDQTVLDAKKNGYTKTMFGRIR
                     PIPELNSSNFMQRQFGERVAMNSPIQGTAADIIKIAMIRVHDRLLDEGLKSRLILQVH
                     DELLIETKEVEKDKVIKLLEKEMRGAVDMKVSMEVGTECGYDWYDAH"
     gene            2909..3550
                     /locus_tag="DHV81_02060"
     CDS             2909..3550
                     /locus_tag="DHV81_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCI93309.1"
                     /translation="MTGMMRIDDRKGNSMIFIGITGGVGAGKSAVLSYLRELDGVRVM
                     LSDEIAHELMEPGSDCYNRLKELFAEEPIWLEDGHFDRPALAGVIFSNDKKRVLLNEV
                     VHPAVKEYVLNAVKEAKKEGLFMLVLEAALLIEEGYGEICDELWYIYASEEVRRKRLK
                     SSRGYSDEKIDSIFASQLKEAEYRRHCKEVIDNDGDIENTIASINKALSKYKE"
     gene            3556..5730
                     /locus_tag="DHV81_02065"
     CDS             3556..5730
                     /locus_tag="DHV81_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCI93310.1"
                     /translation="MNNVFGLDIGTRNVVGTVGYQTDDKEFVVTAQYVREHETRAMLD
                     GQIHDIGRVAKTIKEVKDELEKQTGQPLEEVCIAAAGRVLKTVTTHVEYEYAQESVVT
                     GEDVHTLDLLGIEKAQEALKEVNDTSYKFYCVGYSTVKFFLNDEVFISLEGHKANKIG
                     EDIIVTFLPEDVVDGLYAAVGQAGLSVANMTLEPIAAINVAIPENYRMLNIALVDVGA
                     GTSDISITRDGSIIAYGMIPHAGDELTEVIVQHFLVDFNMAESIKLQSTTSDTVTYKD
                     IMSIEHTIPAQDVWDVAAPVVDNIAQEVSTKIRELNGDKTVSACFVVGGGGKIHGFTE
                     KLAEDLDLPEERVALRGEEVLGDVTFEQEDIKKDPLLVTPIGICLNYYDQRNNFIMVR
                     FNGERIKLYDNNRLTIVDAALQAGFPNDELFPKRGTPINFTVNGVARLVRGEAGEGAV
                     VTMNGKPASINTPLEPNSEIIIEPSTAGEAAVYKISQLDEYNHSVITFVINGRKVSCP
                     RFVQVNGELEPEDYSIRENDVIETRNYYTVRQIAQFMDLVIDTDQMIFVNNEEADLDT
                     LVYENFSVEWKTDEYGVARIDNNNYNDTQESDNTVARTSEQMMNQVLDELHDDFAKEA
                     EASAVPENELSENELPKNDIQEEIHEEDSSENTITVIVNGEPVELSGKDTYIFVDIFT
                     HISFDLQAGKGRAIATVINGRDAQFSEELHEGDQIELYWKEN"
     gene            5732..6535
                     /locus_tag="DHV81_02070"
     CDS             5732..6535
                     /locus_tag="DHV81_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCI93311.1"
                     /translation="MLELCSIASGSSGNCICVGTDDCHVLIDAGISGKKIENGLNEFD
                     MTATDMQGILVTHEHIDHIAGLGVMARRYHIPIYATKATIGAIKCTKSVGNIDDELFH
                     PVEAGVDFTIGDIEFEPLHISHDAADPVAYRFKNENHAMAVVTDLGTYDDALVSKLQN
                     LDGLLLEANHDINMLQTGAYPYPLKRRIMGDSGHLSNERSGQLLCELLHDKFGSVILG
                     HLSKENNYEKLAYEAVRLEVTMGDNPYKASDFPMYVAKRDRASDRIAVE"
     gene            6558..6842
                     /locus_tag="DHV81_02075"
     CDS             6558..6842
                     /locus_tag="DHV81_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCI93312.1"
                     /translation="MDNTKNKVIITVVGKDTVGIIARVCTYLSEGSVNVLDISQTIVS
                     GYFNMMMIVDMANATASFAEISDQLDALGEEIGVTIKCQKEEIFEMMHRI"
     gene            6886..8250
                     /locus_tag="DHV81_02080"
     CDS             6886..8250
                     /locus_tag="DHV81_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HCI93313.1"
                     /translation="MINIGEVIETNDMVEKENLDVRTITLGISLLDCIDSDLAKVNEN
                     IYNKITTLAKNLVSTGKEIENNYCIPIVNKRISVTPIALVGGAACKSSEDFVTIAKTL
                     DRAAKEVGVNFIGGYSALVSKGMTKADELLIKSIPQAMLETDLVCSSVNVGSTKTGIN
                     MDAVKLIGEMIKETAEVTKHLPVSGCAKFVVFCNAPDDNPFMAGAFHGVTEADAIINV
                     GVSGPGVVKTALEKVRGENFEVLCETIKKTAFKVTRVGQLVAKEASKKLGVPFGIIDL
                     SLAPTPAIGDSVADILCEIGLERAGAPGTTAALALLNDQVKKGGIMASSYVGGLSGAF
                     IPVSEDQGMIDAVNDGALTLEKLEAMTCVCSVGLDMIAIPGDTKATTISGIIADEMAI
                     GMINQKTTACRLLPAIGKDVGDVVEIGGLLGSAPVMPVNRFSCDAFVNRGGRIPAPIH
                     SFKN"
     gene            8551..9264
                     /locus_tag="DHV81_02085"
     CDS             8551..9264
                     /locus_tag="DHV81_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine monophosphate cyclohydrolase"
                     /protein_id="HCI93314.1"
                     /translation="MERVSLAKELSSTTYPGRGIVIGRTKDGKKAVTAYFIMGRSENS
                     RNRVFVEDGEGIRTQAFDPSKLEDPSLIIYAPVRVLGNKTIVTNGDQTDTIYELMDKQ
                     QTFEQALRTREFEPDAPNYTPRISGIMHIDNGEFNYAMSILKSNNGNPDACNRYTYAY
                     SAPVAGEGHFIHTYMGDGNPLPSFEGEPTWVDIDGDIDEFTDMVWENLNEDNKVSLFV
                     RYIDIATGEYESRIVNKNK"
     gene            9324..10502
                     /locus_tag="DHV81_02090"
     CDS             9324..10502
                     /locus_tag="DHV81_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HCI93315.1"
                     /translation="MQEYELKYGCNPNQKPARIFMKEGELPIKVLNGRAGYINFLDAF
                     NGWQLVRELKKATGLPAATSFKHVSPAGAAVGQPLSDVEKKIYWVDDMDIEFTPLANA
                     YIRARGADRMSSFGDFISLSDVCDKSTALVIKREVSDGVIAPGYTDEALEILKAKKKG
                     NYCVIEIDPSYEPAPIERKDVFGITFEQGRNELHIDDDFFSNIVTENKELTEQAKIDL
                     AISMITLKYTQSNSVCYVKGGQAIGIGAGQQSRIHCTRLAGSKADNWWLRQSPQVLGL
                     QFVDGIRRADRDNTIDLYIGEDYMDVLAEGEWQKFFKVKPDVFTAEEKRAWLDKNTDV
                     ALGSDAFFPFGDNIERAHKSGVKYVAQPGGSVRDDHVIDTCNKYGMVMSFTGIRLFHH
                     "
     gene            10622..>11259
                     /locus_tag="DHV81_02095"
     CDS             10622..>11259
                     /locus_tag="DHV81_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCI93316.1"
                     /translation="MSLQFITGNSGNGKTKYLFNRIVKEAKANPRDNYLVIVPEQFTM
                     QTQRQLVDLSENKAIMNIDVLSFKRLAYRVFDELGITNLTVLEETGKNLVLRKLAAQE
                     ADKLGVIGRNLNRIGYISEVKSLLSELVQYNISPEELGRYIASGRLSDTLSDKLKDVL
                     VLYEAFEKFMQDKYITAEEILNVLSNVAEESAILRNSVIAFDEFTGFTPIQN"
BASE COUNT         3576 a         2039 c         2885 g         2759 t
ORIGIN      
        1 atagatctcg atgcgattga tccaaatcag atgacagaca gggaaaaagg atacctgaat
       61 gcttatcatg cgagagttta tgagcttgtt tcaccatttt tgaatgatga ggaggctcag
      121 tggcttaaga aatatacaag agctatttaa cattactgcc tgcactgcag tgtgaccggc
      181 agcgattaca acaatattaa gtaataagga aagtataaaa tgagtgaaaa gcttgtactt
      241 atagacggac acagtatttt aaacagggca tatcacggac ttcctgatct gaccaattca
      301 gaggggcttc acacaaatgc agtctatgga tttttgaata tcatgttcaa gattcttgac
      361 gaggaaaagc cagactatct gacggtggca tttgatgtgc atgcgccgac gtttcgccac
      421 agaatgtttg atgcgtacaa gggcacgaga aagcctatgg acgaggagct caggcagcag
      481 gtgcctatga taaaggaaat gcttaccgct atgggcatta aaatcgtgga aaaggaaggc
      541 tatgaggccg acgatatact tggaacctta tctgtcaggg cagaaaaggc cggtatggat
      601 gtcgccatca tttccggaga cagagacctt ttgcagcttg cgacagacca tgtcatggta
      661 aggattccaa agaccaaaaa gacaggaacc gagatagaaa actacaatac agcagatgtt
      721 atcgaaaagt acggcgtcac tccaaaggag tttatcgatg taaaggctct gcagggagat
      781 acgtctgata atattcccgg ggtacccggt atcggcgaaa agacagcagg cgcacttata
      841 gcaaaatatc actgtataga ggcagtacat gaggacgctg agaatgtaaa gccgccaagg
      901 gcttcaaaga atatagtgga gtattgggag caggcgctta tgagcaagga gcttgcgaca
      961 atcatacttg atgcgccggt tgactatgaa ttttctgatg caaagcttag tggcggagta
     1021 gagtcactct acacggagga agccttttta ctgtgcaaaa gatatgaatt caaaaacatg
     1081 ctgtcacggt ttaatgtgga ggctcctaaa aacaatgccg aggagcattt tgtggtagta
     1141 agggacttaa agactgcaga gagagtattt gagaaggcaa aggacaagga ggtcgctttt
     1201 gcggtagtac ctggagagtc tttggaaaat agcgaaagtg atggacagct aagtctgttt
     1261 tcggagccgg tttctaacga ttatcttgct gtttcgattt gtttttcaga ggaggacatt
     1321 tattttatct gtacaggtga tgagatttca agcagcttca tcgatgaaaa actaaatacc
     1381 ctggaagtaa agtcatggat atcaccggat ttaaagacca atctgcacag atttaagagc
     1441 aaagagataa aggcagatga cagaggcaga tattttgaca tgatggtggc tgcatacctt
     1501 atcaatcctc ttgtcgggga gtatccctat gatgcggttg caaaggacta tctggggctt
     1561 atgctctcat ctaaaaagga ttatctggga aagcttgatt tcacccggat gatgaaggag
     1621 gatgagaaaa aggcagtgga ctgtgcctgc tacgaggtgt atactgcctg gaaatcaaag
     1681 ccggttcttt tgcagaagct aaaggaaatg gatatgctca cgctttataa agatatcgag
     1741 ctcccgctcg ttttcgtgct gtatgatatg gaaaacgagg gtataagggc tgatggaata
     1801 aagcttaagg agtatggaga caagcttgct gtttcgataa ctgagcttga aaagaaaata
     1861 tatgaggcag caggtgaaga gtttaatata aattcgccaa agcagcttgg ggtcattctc
     1921 tttgaaaagc ttggactgcc aaatgaaaag aagacaaaga ccggttattc gactgcggca
     1981 gatgtgcttg aaaagctggc gccggagtat ccgattgttg cagatattct ggaatacaga
     2041 cagctcacaa agctaaagag cacatatgct gacggacttt cgggatatat tgcatccgat
     2101 ggcaaaatcc atactacctt aaatcagaca atcactgcga caggccgtct ttcaagcaca
     2161 gagcctaatc tgcagaatat cccgataagg attgagctgg gcaagcttat cagaaaggta
     2221 tttctgcctg aggatggaga tttgtttgtt gattcagact actcgcagat cgagcttcgg
     2281 gtgctggcag cgctttcagg ggatgaaagg atgatagagg cctttaagaa tggtcaggat
     2341 atccacagaa gtacagcatc ccttgtgttt gacacaccgt ttgatgaggt cacagacctt
     2401 caaagacgaa atgccaaggc ggtaaatttt ggcatcgttt atggtatcag cgcgtttggt
     2461 ctgtctaacg acctggggat ttcaaggaaa gaggcacagg gctatataga cagctatttt
     2521 gtcaaatatc caaagattaa ggagtttttg gatcagacgg ttttggatgc caagaaaaat
     2581 ggctatacaa agaccatgtt tgggcgtatc agaccgattc ccgagctgaa ctcgagcaat
     2641 ttcatgcaaa gacagtttgg agagagagtt gcgatgaact cacctattca gggtacagct
     2701 gccgatatca taaagattgc catgatacgt gtgcatgaca ggcttcttga cgagggctta
     2761 aagtcaagac ttatccttca ggtgcatgat gagcttttaa ttgagacaaa ggaagttgag
     2821 aaggataaag tgattaagct tctggagaaa gagatgcgtg gtgcggtgga catgaaggta
     2881 tccatggagg tgggcaccga gtgtggctat gactggtatg atgcgcattg atgacagaaa
     2941 aggaaacagt atgattttta taggaataac aggtggtgta ggtgccggaa aatcggcagt
     3001 gcttagctat cttagggaac ttgatggcgt aagggtcatg ctctccgatg agatagcaca
     3061 tgagctgatg gagccgggaa gcgactgcta caacagatta aaggaattgt ttgcagagga
     3121 accgatatgg cttgaggacg ggcattttga cagaccggca cttgccggag tgattttttc
     3181 gaatgataaa aaaagagtgc ttctaaatga agtagtgcat cctgcagtaa aggaatatgt
     3241 gcttaatgca gttaaagagg caaaaaaaga aggtcttttt atgcttgtgc ttgaagcagc
     3301 actccttata gaggagggat atggtgaaat ctgcgacgag ctgtggtata tttatgcaag
     3361 tgaagaagtc agaagaaaaa gacttaagtc atcacgcgga tattcagatg agaagataga
     3421 tagtatcttt gcaagccagc ttaaagaagc tgagtacaga aggcactgca aggaggttat
     3481 cgataatgat ggagatatcg aaaacacgat agcttcaata aataaagcgc ttagcaaata
     3541 caaggagtag tggaaatgaa taatgtattc ggtttggata ttggtacaag aaatgttgta
     3601 ggaactgtcg gctatcagac tgatgacaag gaatttgtgg ttacagcaca gtatgtcagg
     3661 gagcatgaga ccagagcgat gcttgacggt cagatacatg atataggcag ggtggcaaaa
     3721 accattaagg aagtaaagga cgagcttgaa aaacagacag gacagccact tgaagaggtg
     3781 tgtatagcag ccgcaggacg agtattaaag actgtcacga cgcatgtgga gtatgagtat
     3841 gcgcaggagt ctgtggtgac cggcgaggat gtccatacgc ttgacctgct tggcatagaa
     3901 aaagcacagg aggcactcaa ggaggttaac gatacaagct ataagtttta ctgtgtggga
     3961 tattctacag taaaattctt tttgaatgat gaggtgttta tcagccttga ggggcataag
     4021 gcaaacaaaa taggcgagga tatcatagtt acatttctgc cggaggatgt ggtagacgga
     4081 ctgtatgcgg cagtgggaca ggccgggctt agtgtagcca atatgacatt ggagcctatc
     4141 gccgctatca atgtggcaat tccggagaac tatcgtatgc ttaatatcgc acttgtcgat
     4201 gtgggagccg gaacgagtga tatctcaatc acaagagacg gaagtatcat agcctatggc
     4261 atgataccgc atgcaggaga tgagctgaca gaagttatcg tgcagcattt cctcgtggat
     4321 ttcaatatgg cggagagcat caagcttcag tctacgacaa gtgatacggt tacatacaag
     4381 gatatcatga gcatcgagca cacaattcct gcacaggatg tctgggatgt ggcagcacct
     4441 gttgttgata atattgcaca agaggtatcg actaaaatca gagagctaaa cggtgacaaa
     4501 acagtgagtg cctgctttgt agtcggaggc ggaggcaaga tacacggctt cacggaaaag
     4561 cttgcggaag acctcgacct gccggaggaa agagtagctc taagaggtga ggaagtgctt
     4621 ggagatgtga cctttgagca ggaggatatt aagaaggatc cgcttcttgt cactccaatt
     4681 ggtatatgcc tgaattatta tgaccagaga aataatttta tcatggtcag attcaacggt
     4741 gagagaataa agctctatga caataacagg cttacaatag tcgatgcggc gcttcaggcg
     4801 ggatttccaa acgatgagct gtttccaaag cgtggcaccc cgataaactt tacggtaaat
     4861 ggtgtggcaa gacttgtaag aggcgaagca ggagagggcg cagtggtgac catgaacgga
     4921 aagcctgcaa gcatcaatac accacttgag ccaaacagtg agataatcat agagccgtcc
     4981 acagcaggag aggcagcagt gtataagata tcgcagcttg atgagtacaa tcattctgtc
     5041 attacatttg tcataaatgg cagaaaggta tcatgtccta ggtttgtcca ggtaaacgga
     5101 gagcttgagc cggaggatta cagcatcagg gaaaatgatg tgattgagac aagaaattac
     5161 tatacagtgc gccagattgc gcagtttatg gacctcgtta ttgacacaga ccagatgata
     5221 tttgtaaaca atgaagaggc tgatctcgac actctcgtat atgaaaattt ctctgttgag
     5281 tggaaaacag atgaatatgg tgttgcaaga atcgacaata acaattataa tgacacacaa
     5341 gagtctgaca acacagtagc cagaacctca gagcagatga tgaatcaggt gctcgatgag
     5401 ctgcatgatg attttgctaa agaagctgaa gcatctgctg tgccggaaaa cgaactgtcg
     5461 gaaaatgaat tgccaaaaaa tgatatacag gaagaaatac acgaagaaga ctcatcagaa
     5521 aatacaatta cagtaattgt aaacggtgag cctgtagagt tgtccggcaa ggatacatat
     5581 atatttgtcg atatatttac ccatatctca tttgatttac aggcaggaaa gggcagggca
     5641 atagccactg taataaatgg cagagatgct cagttttccg aggagcttca tgagggagac
     5701 caaatagaat tatactggaa agagaattaa catgttagaa ttatgcagca ttgccagcgg
     5761 cagcagtgga aactgtatat gtgtgggtac ggatgattgt catgtgctca tagacgcggg
     5821 catcagcggt aagaaaatag aaaacggcct gaatgaattt gatatgacgg ccacagatat
     5881 gcaggggatt ctcgtgacac atgagcatat agaccatatc gcagggcttg gtgtcatggc
     5941 aagacgctat catattccga tttatgcaac aaaggccaca atcggggcca taaaatgtac
     6001 caaatccgtt ggaaatattg atgatgagct gtttcatcct gttgaggcag gcgtcgattt
     6061 tacaataggc gatatagagt ttgagccgct tcacatatca cacgatgcgg ctgatcctgt
     6121 cgcgtacaga tttaaaaacg aaaatcatgc gatggcggtc gtgacagacc ttggcacgta
     6181 tgatgatgca ctggtttcaa agcttcagaa ccttgatggg ctgctgcttg aagccaatca
     6241 tgatataaat atgctccaga caggggcata tccgtatccg ctcaagcgca gaatcatggg
     6301 tgacagcgga cacttgtcga atgagagaag cgggcagttg ttatgcgagc ttcttcatga
     6361 taaatttggc tcagtaatac tgggacacct tagtaaagaa aacaactacg aaaagctggc
     6421 gtatgaagct gtcaggcttg aggtcacaat gggagacaat ccatataagg caagtgattt
     6481 ccctatgtat gtggctaaga gagacagggc atcagaccgc atagccgtag aataagagaa
     6541 caggagaata agttgttatg gataacacaa agaacaaagt aatcatcaca gttgtcggaa
     6601 aagacacagt aggaatcatc gcaagggtat gtacatatct gtcagagggc agtgtaaacg
     6661 tgcttgatat ctcacagact atcgtttcag gttatttcaa catgatgatg attgttgata
     6721 tggcaaatgc tacagcttca tttgcagaga tttctgacca gcttgatgca cttggagaag
     6781 aaattggtgt aacaataaag tgtcagaagg aagaaatctt tgagatgatg catcgaatct
     6841 agtttagcat aaaaatgtca tacacagtta aaggagaaca gcaaaatgat aaatattggt
     6901 gaagtaattg agaccaatga catggtggag aaggaaaatc ttgacgtgcg tacaatcaca
     6961 ctcggtatca gcctgctcga ctgtatagac tcagaccttg caaaggtaaa tgagaatatc
     7021 tacaacaaga ttacaacact tgcaaagaat cttgtatcaa caggaaaaga gatagagaac
     7081 aactactgta tccctatcgt aaacaaacgt atttcagtta cacctatcgc ccttgtcggc
     7141 ggtgcggcct gcaagagctc agaggatttt gttaccatag caaagacact tgacagagct
     7201 gccaaagagg taggggtcaa cttcatcggt ggatactcag cacttgtgtc aaagggcatg
     7261 acaaaggcgg atgagctttt aatcaaatcc atccctcagg caatgcttga gacagacctg
     7321 gtatgcagct ctgtaaatgt tggctctaca aaaaccggaa tcaacatgga tgcagtaaag
     7381 ctgattggcg agatgataaa ggagacagca gaggtgacaa agcaccttcc tgtatcaggc
     7441 tgtgccaagt ttgtagtatt ctgcaacgct cctgatgaca atccgtttat ggcaggagca
     7501 ttccatggtg tgacagaggc agatgccatc atcaatgtag gtgtcagcgg accgggagtt
     7561 gtaaagactg ctctcgaaaa ggtaagaggc gagaactttg aggtgctctg tgagacaatc
     7621 aagaagacag ccttcaaggt tacacgagta ggacagcttg tggcaaagga ggcttcaaag
     7681 aagcttggag tgccatttgg aatcatagac ctctcacttg cacctacacc ggctatcgga
     7741 gactctgtag cagatattct ctgcgagata ggcttagaga gagccggagc tccgggaaca
     7801 acagcagcac ttgcactctt aaacgatcag gtgaaaaagg gcggcatcat ggcatcctca
     7861 tatgtaggcg gactctcagg tgcttttatc ccggtatcag aggatcaggg aatgatagat
     7921 gctgtaaatg atggtgcact cactcttgaa aagcttgagg ctatgacctg tgtatgctca
     7981 gtaggacttg acatgattgc aatcccgggc gatacaaagg ccaccacaat ttcaggaatt
     8041 atcgcagacg agatggctat cggtatgata aaccaaaaga caacagcctg cagactgctt
     8101 ccggcaatag gaaaggatgt aggtgatgtg gtagaaatcg gaggactcct tggaagtgca
     8161 ccggttatgc cggtaaacag attctcttgt gatgcatttg taaaccgtgg aggaagaatc
     8221 ccggcaccta ttcacagctt taaaaattag taaataatgg cattgtttac aagcatgtac
     8281 ttataagaat aacttataag tacatgctta ttttaaagtg gtacttgaca atgacaaaaa
     8341 tacatgctta aataaagaca gttatatgac tgacggaagt ggataaccac atggagtata
     8401 acagttgatt gccgaccgtc tgggcgcaca tttatataag tttcctgcag agctttatat
     8461 ttttatgtgt gttcagacgg tctttttatt ccggcaggcc cgagtaattt ggggatgcag
     8521 accacattat cagaaaacat ggaggatgac atggaaagag tatcattagc aaaggaatta
     8581 tcaagcacaa cataccctgg cagaggtatc gtaatcggcc gcacaaagga tggtaagaag
     8641 gctgtaacag catactttat catgggcaga agcgaaaaca gccgcaacag agtatttgta
     8701 gaggatggcg agggcatcag aacacaggca tttgaccctt caaagcttga ggatccaagt
     8761 cttatcattt acgcacctgt aagagtgctc ggcaacaaga caattgtcac aaacggtgac
     8821 cagacagata ctatctatga gctgatggac aagcagcaga catttgagca ggccttaagg
     8881 acaagagagt ttgagcctga cgctcctaac tatacaccta gaatttcagg aatcatgcat
     8941 attgacaatg gtgagttcaa ctatgctatg tcaatcttaa agagcaacaa cggcaatcct
     9001 gatgcatgca acagatatac atatgcatac tcagcaccgg ttgcaggaga gggacatttc
     9061 atccatacat atatgggtga tggcaatccg ctgccaagct ttgagggtga gcctacatgg
     9121 gtagacatcg acggagatat cgatgagttt accgatatgg tatgggagaa cctaaacgaa
     9181 gacaataagg tatcactttt tgtcagatat attgatatcg caacaggcga gtatgagagc
     9241 agaatcgtaa acaaaaacaa ataaaacact atataaacgg tcaaatatac aagataagca
     9301 gataaaaaca ggagaacaga aaaatgcagg aatacgaatt aaaatacgga tgtaatccaa
     9361 atcagaaacc agccagaata tttatgaagg agggagagct tcctataaaa gtgcttaacg
     9421 gcagagccgg atatatcaac ttccttgatg cctttaacgg atggcagctt gtaagagagc
     9481 ttaagaaggc aacaggtctt ccggccgcaa catccttcaa gcatgtttca cctgcaggtg
     9541 cagcagtagg acagccactt tcagatgtgg agaaaaagat ttactgggtt gatgatatgg
     9601 atattgagtt tacaccactt gcaaatgcat acatcagagc aagaggagct gacagaatgt
     9661 catcatttgg tgattttatt tcactttcag atgtatgtga caagagcact gcacttgtaa
     9721 tcaagagaga ggtatcagac ggtgttattg ctccgggcta cacagacgag gctcttgaga
     9781 ttttaaaggc taagaaaaaa ggcaattact gtgttatcga gattgacccg tcatatgagc
     9841 cggcacctat cgagagaaag gatgtatttg gcatcacatt tgagcagggc agaaacgagc
     9901 ttcatattga tgatgatttc ttctcaaata ttgtgacaga gaacaaggag ctcacagagc
     9961 aggctaagat cgatctggca atatctatga ttacattaaa gtatacacag tccaactctg
    10021 tatgctatgt aaagggtggt caggcaatcg gtatcggagc aggccagcag tctcgtatac
    10081 actgtaccag acttgcaggc tcaaaggcag acaactggtg gcttcgtcag tctccacagg
    10141 tgcttggact tcagttcgtg gatggtatca gacgtgcaga ccgcgacaat actattgacc
    10201 tttatatcgg tgaggattat atggatgtgc ttgcagaggg tgagtggcag aagttcttca
    10261 aggtaaagcc ggacgtattt acagcagagg agaagagagc ttggcttgac aagaatacag
    10321 atgtagcact tggctcggat gcattcttcc catttggtga caacattgag cgtgctcaca
    10381 agagtggcgt gaagtatgta gcccagccgg gtggatctgt gagagatgac catgttattg
    10441 atacatgtaa taagtacggt atggtaatga gctttacagg aatcagactt ttccatcact
    10501 aatcacaagg catagcggtt attaagctga atttaagcac aataagtaat tcaataatag
    10561 aggacttcat gatataatta cagtatacat tttacacagt agttaatcat ggagtctttt
    10621 tatgtcatta cagtttatca caggtaattc aggaaacgga aaaacgaaat atctctttaa
    10681 caggatagtg aaagaggcaa aggcaaaccc aagggacaat tatcttgtaa tagtgcctga
    10741 gcagtttaca atgcagacgc agagacagct tgtagatttg tcggagaata aagctatcat
    10801 gaatatagat gtgctaagct ttaagcgctt agcctacaga gtgtttgatg agcttggaat
    10861 cacaaatctc acggtgcttg aggagacagg taagaacctt gtactcagaa agcttgcggc
    10921 acaggaggcg gataagcttg gcgtaatagg cagaaactta aaccgcatcg gctatataag
    10981 tgaggtaaag tcactgcttt cggagcttgt gcagtacaat atatcacctg aggagctcgg
    11041 aagatatatt gcctcaggca ggctatcgga tacactttca gataagctca aggatgtgct
    11101 cgtgctctac gaagcatttg agaagtttat gcaggacaaa tatatcactg cagaggaaat
    11161 acttaatgtg cttagcaatg tggcggagga gtctgccatt cttagaaatt cagtcattgc
    11221 atttgatgaa tttaccggat ttacacctat acagaacca
//
LOCUS       DPUF01000059           10626 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_5315, whole
            genome shotgun sequence.
ACCESSION   DPUF01000059 DPUF01000000
VERSION     DPUF01000059.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_5315"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="DHV81_02100"
     CDS             <1..364
                     /locus_tag="DHV81_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93317.1"
                     /translation="KHGEDTYEWCRPVMQVKEYAMKAKEKGTKLYVLTTSGTKIETAA
                     KRRFLDRYYAGMFDDIYAVEHDDDKVRFILKKAAELGIEPSDCELVEDTYRILLQTVT
                     EGIKSTHITTILTGGCER"
     gene            476..751
                     /gene="flgM"
                     /locus_tag="DHV81_02105"
     CDS             476..751
                     /gene="flgM"
                     /locus_tag="DHV81_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HCI93318.1"
                     /translation="MRIDAYNQIAQLYGVSRTTKTAKTQSASRMSDQVSISQAGRDYQ
                     IAKKAVSEASDIREDKVALLKNMVDSGQYQVSGDDFASKLLERYNSL"
     gene            808..1308
                     /locus_tag="DHV81_02110"
     CDS             808..1308
                     /locus_tag="DHV81_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HCI93319.1"
                     /translation="MEELINVLTEEEKVYRTLAANGEKKRQIIIDADIPALEAITDLD
                     QQAGDELLIMSNKQVSLLTDIANVLGKSDEKMTVTRLIGYLGTQPDIQAKLTAARDSL
                     IEAAAQMKEINDLNSQLLAQAIELTEFDITLFKSMKQAPETANYDRNAYNTGDILGSS
                     GFDAKQ"
     gene            1332..3041
                     /gene="flgK"
                     /locus_tag="DHV81_02115"
     CDS             1332..3041
                     /gene="flgK"
                     /locus_tag="DHV81_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCI93320.1"
                     /translation="MANGFGSFYVGSSGLKNSQNALNTTANNMANVNTTGYVRQQVRF
                     SDKHYITRNNPTAGTNIQQSGLGVSVSDVAHVRDIFLDKSYRQENGRKGFYSTLYSTT
                     SYVEDIMQEMNGAEFKESISELWQAFQEYGKDPTNSTNQNLIIQKSELFLTRCNTVYS
                     DLQKYQSNINLQIKDQVDRINEIGDKIYALNLEIQKTESGGVETAMTARDARDSLIDE
                     LAGYAKIEAEEDSTGYVRIKLEGQQFVQDNKCNHIGLTEEKGTGFYTPYWPHITTQES
                     YMPVFSDVALPSALAERVGCTQTTNIATSLNNDIGSLKALLVSRGSEYGTYDFMSEEN
                     YSRVEDNVVMETQAEISYLARNIMMAMNDIFCPNTTYTAADGTTYTVLDTANCSVGAD
                     GSLPPHELFAREGYDRYTQMEIDGKQFYVYNDETKANNSSWYKLGNVSVNPKLAAQVT
                     LMPSYKQDGAANYGLADQITKAWSAENLYINPKDTSKCNFEGYYDKIISHLGTNGSIY
                     KASSDTMTSTAAAIDNQRNQIMGVSSDEELTNMIKYQSAYNASSRFITVISQMTELIV
                     QLI"
     gene            3083..4936
                     /gene="flgK"
                     /locus_tag="DHV81_02120"
     CDS             3083..4936
                     /gene="flgK"
                     /locus_tag="DHV81_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCI93321.1"
                     /translation="MASTFFGLHIGSSALSSFQVATNTTANNIANVKTTGYTRQEATL
                     QAKDAINVTARYGSVGTGVTVTSIKQQRDLYYDNKYWENNSCYGRYEQKLYYMEQIQN
                     YFKDNGSSVKGFSSVFSQMFSDLDTLRSKPSDKTVRNQFISSAQSLCTYFNQMSDNLS
                     KLQDDCNEEIRNNVDKINSISEKISLLNKEINQIETGTGVEASSLRDERANLIDSLSK
                     IVNVSYNETEVQNTNGDNLGGTNFSLYINGEKVVEGKDYRKLICESSKTKNNQTDNDD
                     MYKIYWEDTKMEFSATAGTAGGSLKALFEVRDGDNLENFKGKVTKADSYSLTVENISI
                     DNIKSLNLPDKDGKITVNNISYSYDSWEAQVDAQGNIKSVTFNLSKDKAIADPEKTVA
                     EGYLLNAGSAINARGIPYYMTQLNEFVRNFSEMFNQIESKGQNLNGDTPPTFFEAITN
                     TAKVYDFSESEAYSKLPDGQTATINSSSNTYYRMTAANFSVNKDVMNDVSLFATATDY
                     VKTDSCDIVDELKKLQSEKTVYRGDKAESFLETIISNVSVDTEKAETYNKLYSNLEQT
                     IANQRTSVSGVDEDEEALNLVKFQYSYNMASKIISVMNQMLDKLINDTGVA"
     gene            4953..6485
                     /locus_tag="DHV81_02125"
     CDS             4953..6485
                     /locus_tag="DHV81_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgL"
                     /protein_id="HCI93322.1"
                     /translation="MRITNNMIMGNTKTNINSTKVLVDKYNTQMTTQKKISKASEDPV
                     IAIRSLRLSTSLSHLDQYKDNNIPDASSWMDVTETALSNMKSLLTDIRTQCVNGSTDT
                     LTADDRNTILQQLTALSEQVYTEGNADYAGRTVFTGYRTSSKLTFQKDTKSTYQITQE
                     FSAADLSEKRYYTNGVTVPNSINAATPDCTTDVTEHSFQRIRLGYGNTTDDVKLSIDG
                     KELTPGTDYTVYKSVSDFDPSKLSADDTAYIQETGEFVFGKNVAASIKDNDKKLSVTY
                     VKTGFDSSDARPEYYYNCKDITNAVTLDAGGNVPHDAAGDIIYSDPSKVVDFKFSSQE
                     IKYTVANSTDITVNTQAKDVMDTGIKRDVDELIDVVQNAVNAHDKVSQIKKMMQQQQY
                     SDKDSQAKLKTYLEAAEQEADYADNNLQKTYSQYITRFDDHLNKVNLALTNSGSTKSR
                     LTLIKNRVEEQQTTIEELKSTNEDRDISDIIIDFYAMYNAYQSSLTAASKANSQTLLD
                     YL"
     gene            6555..7019
                     /locus_tag="DHV81_02130"
     CDS             6555..7019
                     /locus_tag="DHV81_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HCI93323.1"
                     /translation="MRAKTRIFGEIDIPQDKIITMEKGMIGFSELKNYTLIYNSEKEN
                     DKKSIMWLQSMDDGDIAFPVMTPDIIMPDYKPTVNEELLAPLGDLNEENMYVIVTVNV
                     PSDITKIACNLKAPIVVNTDSNKAAQLIVEDDYKVHFPIYDIIKGKKERAGE"
     gene            7021..7239
                     /gene="csrA"
                     /locus_tag="DHV81_02135"
     CDS             7021..7239
                     /gene="csrA"
                     /locus_tag="DHV81_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HCI93324.1"
                     /translation="MLALTRKKGESLVINNDIEITILEIRGDQIKLGVSAPKEVPIYR
                     KEVYTQIQQENRKSADAQNAQALKELFG"
     gene            7416..>7546
                     /locus_tag="DHV81_02140"
     CDS             7416..>7546
                     /locus_tag="DHV81_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI93325.1"
                     /translation="MEKVRYMISDAAAAVDVETHVLRYWEDELGLDVPRNELGHRYYT
                     "
     assembly_gap    7547..7718
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7719..8180
                     /locus_tag="DHV81_02145"
     CDS             <7719..8180
                     /locus_tag="DHV81_02145"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93326.1"
                     /translation="VQPVNYTNITNQTVKQEMSGYNPAYHDELPGYNHTYRSRTADER
                     MDEFRELMSNIVGRAIALNNEELSQQISTEVQERILKEMNYLMREQDIAQEERYKKLD
                     AAIRGNLRRKGLFARKRNADMPADTKISAADTKGKKGRDKTHTHELNPVRL"
     gene            complement(8327..8497)
                     /locus_tag="DHV81_02150"
     CDS             complement(8327..8497)
                     /locus_tag="DHV81_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCI93327.1"
                     /translation="MAYVISDSCVSCGTCAGECPVGAISAGDSQYVIDADSCLDCGTC
                     AGVCPTGAISQG"
     gene            8859..10367
                     /locus_tag="DHV81_02155"
     CDS             8859..10367
                     /locus_tag="DHV81_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCI93328.1"
                     /translation="METKIIKIDQDNLDHKLMQEAGDLIAAGELVAFPTETVYGLGGD
                     ALDPEASKKIYSAKGRPSDNPLIVHISDFSDLERIAKTVPEDARKLSDAFWPGPLTMI
                     VEKGDAVPYATTGGMDTVAVRMPNHPIALDLIRRSGCLIAAPSANTSGRPSPTEAAHV
                     AEDLSGKIAMIIDGGPVGIGIESTIIDLTEDTPMVLRPGYITPQMLSKVLGKEVVIDP
                     GIIAADDTRKPKAPGMKYKHYAPKADMVIVDGTRKHVIAKINELVASHRDDGKKIAVI
                     ATEETKQFYDADVVLSMGSRADEDSIAHELYRILRDCDELDVDVIFSESFSTPRIGQA
                     IMNRMLKAAGHQVIDTHVKYDKIIFVAQTGTCREQMAKGIMNDFVLKVPMEIEARGLV
                     VQFPEPVNQKAEAVLISNGISTEGMVSTQLEESDITESTMVFTMESSQRERIIESFAD
                     IDPEQVFVLSQYVGDELEILDPYGGTLQSYGLCYESLRATLKKLVKLLNANT"
     gene            10415..>10626
                     /gene="upp"
                     /locus_tag="DHV81_02160"
     CDS             10415..>10626
                     /gene="upp"
                     /locus_tag="DHV81_02160"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741040.1"
                     /note="Catalyzes the formation of uracil and
                     5-phospho-alpha-D-ribosy 1-diphosphate from UMP and
                     diphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCI93329.1"
                     /translation="MGKVVVMDHPLIQHKIGIMRRTDTGSKDFRTLVSEVAMLECYEA
                     TRDLELTDVEIETPICKATVKELKGK"
BASE COUNT         3450 a         2087 c         2568 g         2349 t
ORIGIN      
        1 taagcatggt gaggatacat atgagtggtg ccgtcctgtg atgcaggtaa aggagtatgc
       61 catgaaggca aaggaaaaag gcacgaagct gtatgtgctt accacatcag gcactaaaat
      121 cgagacagct gcaaagcgca gatttttaga cagatactat gctggtatgt ttgatgacat
      181 ctatgcggtg gagcatgatg atgacaaggt caggttcatt cttaaaaaag cagcagagct
      241 tgggattgaa ccgtcggact gtgagcttgt ggaggataca taccgcatac ttttgcagac
      301 agtgacagag gggattaaat ctactcacat cacaacgatt ctcacaggcg gatgcgaacg
      361 gtaaccaaaa attttccacg gtctataaag aaataaaaaa aacatccgat aaatatgtca
      421 ggaagcaaaa tttacatatt tcaaggatga gatataatag aaaggagatc ctgttatgcg
      481 tatagatgca tataatcaga tagcccagtt gtatggcgta tcaagaacaa caaagacagc
      541 aaagacacaa agcgcgtcga ggatgtctga tcaggtatca atttcgcagg ccggtagaga
      601 ttaccagatt gcgaaaaaag cagtatcaga ggcttcggac atcagggaag ataaagtcgc
      661 tttgcttaag aacatggtag actccggaca gtatcaggtc agtggagatg atttcgccag
      721 caagctgtta gagaggtaca actctctata atatgataat catatcatat atcaaaggta
      781 aaagaggatt ttatagtggc tagtttagtg gaagagctta taaatgtgct cacagaggaa
      841 gaaaaagtat acagaacact cgctgcaaat ggggagaaga aacgtcagat aatcatagat
      901 gccgatatcc ctgcgcttga agcaataact gatttggatc agcaggcagg cgatgagctt
      961 cttattatga gcaataagca ggtttcactt ctcacagata ttgccaatgt actgggcaaa
     1021 tcagatgaga agatgacagt cacaaggctg attggatatc tcggtacaca gccggatatt
     1081 caggcgaagc ttacggcagc gcgcgacagt ctgattgaag cagctgcaca gatgaaagaa
     1141 atcaacgatt taaactcaca attacttgct caggcaatag agcttacaga gtttgacatc
     1201 accctgttca agagcatgaa acaggcgccg gagaccgcca attatgacag aaatgcatat
     1261 aacacaggcg atatcttagg cagcagtggt tttgatgcaa agcaataatc attaaccggg
     1321 aggacaggaa catggcaaat ggattcggaa gtttttacgt aggtagttcc ggacttaaaa
     1381 actcgcagaa tgctcttaat acaacagcaa acaacatggc caatgtcaat acgacaggct
     1441 atgtgcgcca gcaggtaaga ttttcagaca aacattatat caccagaaat aaccctacag
     1501 cgggcaccaa catccagcag agcggactgg gtgtttccgt atcggatgtg gcacatgtca
     1561 gggatatatt cttagataaa tcgtataggc aggagaatgg tcgaaagggc ttttactcga
     1621 cactttacag caccacatca tatgtggagg atatcatgca ggagatgaac ggtgctgagt
     1681 ttaaggagag tatatcagag ctatggcagg cttttcagga gtatggcaag gatccgacga
     1741 attcgacgaa tcagaacctt atcatccaaa agagcgagct ttttctgacc agatgcaata
     1801 cggtttattc tgatcttcag aaataccagt caaatatcaa cttacaaatt aaagaccagg
     1861 ttgacagaat caacgaaatc ggtgacaaga tttatgcgct caaccttgag attcaaaaga
     1921 cagagtcagg cggtgtagag actgccatga cagcgcgcga tgccagggac agtctgattg
     1981 atgagctcgc aggttacgct aagatagagg ctgaggagga ttccacaggc tacgtgcgta
     2041 tcaagctcga gggacagcag tttgtacagg ataacaagtg caaccatata ggcctcaccg
     2101 aggaaaaggg tacaggcttt tatacacctt actggcctca tattactaca caggagagct
     2161 atatgccggt gtttagcgac gttgcgctgc cgtcagcgct ggctgaaaga gtcggctgca
     2221 cgcagaccac caacatagca accagcctga ataatgatat aggaagcctg aaggcactcc
     2281 ttgtatcaag aggctcagag tatggcacct atgatttcat gagcgaggag aattacagta
     2341 gggtggagga caatgtggtc atggaaacac aggccgagat atcatatctg gcgcgaaaca
     2401 tcatgatggc catgaacgat atattttgtc cgaacaccac atacacagcg gcagacggta
     2461 ccacatacac ggttttggat acagcaaact gctcggtagg agctgatggc agcctgccac
     2521 cgcatgagct ctttgcaaga gaaggatatg accgttacac gcagatggaa atagatggca
     2581 aacagtttta tgtatacaat gatgagacaa aggctaacaa ctcatcatgg tacaagcttg
     2641 gaaatgtttc agtaaatcca aagcttgcgg cacaggtgac actgatgccg tcatacaagc
     2701 aggacggagc cgcaaactat ggccttgcag accagattac aaaggcgtgg tcagcagaga
     2761 atctatacat caacccaaag gatacgtcga agtgcaattt tgagggatat tatgacaaga
     2821 taatctcgca tctcggaaca aacggcagca tatataaggc gtcatcagac actatgacat
     2881 cgacagcagc tgccatagat aaccagagaa accagataat gggtgtgtcg agtgacgagg
     2941 agctgacaaa catgatcaag tatcagtcgg cctacaatgc atcaagccgt tttatcacag
     3001 taatcagtca gatgactgag ctcatagtac agctcatata gttttaggat acgacaagag
     3061 agtatgaaca ggaggagtcg atatggcttc aacatttttt ggattacata tagggtcaag
     3121 cgctctgagc tcatttcagg tagcgaccaa tacaacagcc aacaacatag ccaatgtcaa
     3181 gacgaccgga tacaccaggc aggaggctac cctgcaggca aaggatgcga taaatgtcac
     3241 agcccgctac ggaagcgttg gcacaggtgt tacagtcacc tcaatcaaac agcagcgtga
     3301 cctgtactac gacaataaat actgggaaaa caattcctgc tatggacgct atgagcagaa
     3361 gctgtattac atggaacaga ttcagaacta ctttaaggat aacggttcat cggtaaaggg
     3421 attttcaagt gttttttcac agatgttttc agaccttgat acactgcgca gcaagccgtc
     3481 cgacaagaca gtgagaaatc agtttatcag cagcgcgcag agtctttgta catatttcaa
     3541 ccagatgagt gacaatctct caaagctgca ggatgactgc aacgaagaaa tcagaaacaa
     3601 cgtagacaaa atcaattcaa tcagtgagaa aatttcactg ttaaataagg agattaacca
     3661 gatagagaca ggaaccggtg tcgaggccag ctcacttagg gatgagaggg cgaatctcat
     3721 cgacagcctg tccaagattg ttaatgtatc ttacaacgag acagaggtgc aaaacaccaa
     3781 tggagacaac ctgggcggca caaatttctc gctctatata aatggagaga aggttgtgga
     3841 gggcaaggat taccgaaagc tcatctgtga gtcgtcaaag acgaaaaaca atcagacaga
     3901 caacgatgat atgtacaaga tatactggga agatactaag atggagtttt ccgcgacagc
     3961 tggaaccgca gggggaagcc tgaaagctct ctttgaggtg agagacggtg ataatctgga
     4021 gaatttcaag ggtaaggtca caaaggctga ttcgtattcg ctcacagtgg agaatatatc
     4081 aatagacaat ataaaatcac tcaacctgcc ggacaaggac ggcaaaatca ctgtaaataa
     4141 tatcagctac agctatgaca gctgggaagc acaggtggat gcacagggca atatcaagtc
     4201 tgtaacattt aatctgagca aggacaaggc catagcagac cctgaaaaga ctgtggcaga
     4261 gggatatctt ttaaatgcgg gctctgcaat aaatgcgagg ggaatccctt actatatgac
     4321 acagctcaat gagtttgtca gaaatttttc agagatgttc aatcagatag agagcaaagg
     4381 acagaatctg aatggggata caccacctac attttttgaa gcaatcacaa acacagccaa
     4441 ggtatatgat ttctcggaat cagaggctta cagcaagctg cctgatggac agactgccac
     4501 aataaacagc agctccaaca cgtattacag gatgacagcg gccaatttct ccgtaaacaa
     4561 ggatgtgatg aacgatgtat cgctttttgc gactgccaca gactatgtca agacagattc
     4621 atgtgatata gttgatgagt taaagaagct gcagtcggag aaaacagtat acagaggcga
     4681 taaagcggaa tcattccttg agacaatcat ctccaatgtg tcagtggata cggaaaaggc
     4741 agagacctac aacaagctct atagcaatct ggagcagact attgcaaacc aaagaacatc
     4801 ggtatcaggt gtggatgagg atgaggaggc attaaacctc gtgaagttcc agtattcata
     4861 caatatggca tctaaaatca tatcggttat gaaccagatg cttgataagc tcataaacga
     4921 tacgggcgtg gcatagaagc gggaggcata atatgagaat cacaaacaat atgataatgg
     4981 gaaataccaa gacaaatatc aattcaacaa aggttttggt ggacaaatac aatacccaga
     5041 tgactactca aaagaagata tccaaggcat cagaggatcc ggttatcgca atcagatcgc
     5101 ttcgcttgtc gaccagcctg tcgcatcttg accagtacaa ggacaacaat atcccggatg
     5161 cttcttcgtg gatggatgtc acagagacag cccttagcaa catgaaatca ctcctcacag
     5221 atatccgtac acagtgtgta aacggctcta cagatacgct cacagctgat gacagaaaca
     5281 ctatattaca gcagcttaca gcgctgtcag agcaggtgta tacagagggc aatgcagact
     5341 acgcaggaag aacagtattt acgggctacc gcacatcatc aaagctcaca ttccagaagg
     5401 acacaaagag cacatatcag ataacacagg agttttctgc cgctgatttg tcagagaagc
     5461 ggtattatac aaatggcgtg actgttccaa atagtataaa tgcggcaaca cctgactgta
     5521 ccacagatgt cacagagcac agcttccaga gaatccgtct gggctacggt aatacgacag
     5581 acgatgtgaa gctttctatc gatggaaaag agctgacacc gggcacagat tatactgtat
     5641 acaagagtgt gtctgatttt gacccatcaa agctctctgc tgatgatacg gcatatatac
     5701 aggaaaccgg tgaatttgtt tttggtaaaa atgtggctgc ttcaattaag gataatgaca
     5761 agaagctgtc tgtcacatat gtaaagacag gctttgacag cagtgatgcg agacctgagt
     5821 attattacaa ctgcaaggat attacaaacg ctgtgacact cgatgcaggt ggcaatgtac
     5881 ctcatgatgc agcaggagat atcatatact ccgatccatc aaaggtagtt gattttaagt
     5941 tttcaagtca ggaaatcaaa tacacagtgg caaacagcac cgatatcact gtcaatacac
     6001 aggcgaagga cgtcatggat acaggcatca agcgcgatgt ggatgagttg attgatgtag
     6061 tgcagaatgc tgtcaatgct cacgacaagg tctcacagat aaagaagatg atgcagcaac
     6121 agcagtactc tgacaaggat tcacaggcaa agcttaagac atatttagag gctgcagagc
     6181 aggaggctga ctatgccgac aacaatttgc aaaagaccta cagccagtac atcacacgct
     6241 ttgatgatca tttgaataag gtaaatctgg cactcaccaa ttcgggaagc acaaagagcc
     6301 gcctcactct catcaagaac agagttgagg agcagcagac gaccatagag gagcttaaat
     6361 cgaccaatga ggatagggat atttctgata taatcattga tttttacgct atgtacaatg
     6421 catatcagtc cagtctgaca gcggcatcaa aggcaaactc acagacattg cttgattatc
     6481 tgtaaggatt ttaaaaacgg tacacagcta ccgggctata taacgctcgc ggagcgggcg
     6541 aaaaggagga agccatgagg gcaaaaacga gaatatttgg ggaaattgac atcccacagg
     6601 acaaaatcat cacaatggag aaggggatga taggcttttc ggagctgaaa aactacactc
     6661 tgatatacaa ttcggagaag gagaatgata agaagtctat catgtggctt cagtcgatgg
     6721 atgatggaga cattgcattt cctgtcatga caccggatat catcatgccg gactacaagc
     6781 cgacggtcaa cgaggagctt cttgcgcctc ttggagatct gaatgaggag aatatgtatg
     6841 tgatcgtcac tgtaaatgtg cctagcgaca tcacaaagat tgcctgcaat ttaaaggctc
     6901 ctattgttgt caataccgac agcaacaaag cagcacagct tatagttgag gatgactaca
     6961 aggtgcactt cccgatttat gacattataa aagggaaaaa agaaagggcg ggtgaatagt
     7021 atgctggctc ttacaagaaa aaagggtgaa tctctggtga ttaataatga catcgagatt
     7081 actattctgg aaatcagagg cgaccagatt aagctcggtg taagtgcacc gaaggaggtg
     7141 ccgatttacc gcaaggaggt ttatacgcag atacagcagg agaacaggaa atctgcggat
     7201 gcacagaatg cacaggctct caaggagctg tttggatagc ttaagacaga gagttatatg
     7261 tcgaaagttg cgaccgaaac caattgaatg agagttctat caaggctata attgaaccag
     7321 tacagcgata taactttttt cgcagtgctg gttcttttcg atgtgccaaa agcgtatagc
     7381 gaataaaata taatttcaga caggaggact tcaatttgga gaaagtacgt tacatgattt
     7441 ccgatgcggc agctgccgtg gatgtggaga ctcatgtgtt gcgttactgg gaggatgagc
     7501 tgggacttga tgtgcctaga aatgaacttg gtcacagata ctatacnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gcagcctgta aattacacga
     7741 atatcacaaa tcagacggtg aaacaggaga tgtcagggta taatccggca tatcacgatg
     7801 aactgccggg atacaatcat acatatcgaa gccgcacggc agatgagcgt atggatgagt
     7861 ttcgcgagct gatgagcaat atcgtgggga gagccatagc actgaacaat gaggagctga
     7921 gtcagcagat atccactgag gtgcaggagc ggatattaaa ggaaatgaac tatctgatgc
     7981 gcgaacagga tattgcgcag gaggagagat ataaaaagct ggacgctgcc atacgaggaa
     8041 atctgcgcag aaaggggctt tttgccagga aaaggaacgc ggacatgcct gcagatacaa
     8101 aaataagcgc tgcagacacc aagggaaaga aggggcgcga caagacgcat acgcatgagc
     8161 ttaatccggt gagactatag agcatgcatg acaacgctta cactataaaa ataattactg
     8221 caaaaaacgc ttacagcata aaaatgatta caatacaaag acgattacaa cacaaaaaaa
     8281 gacgacccat aacaggtcgt ctttatttct tacagtatgc taaaaattag ccctggctga
     8341 ttgctccagt tgggcaaaca ccagcacatg taccacaatc gagacaagaa tctgcatcga
     8401 ttacgtactg tgaatcacca gcagagatag ctcctactgg gcactcacca gcacatgtac
     8461 cacagctaac gcatgaatca gaaattacgt atgccataat gaaatcctcc ttataaaata
     8521 atgatcatta ttcgccgtta ttggcttatc tgcattgtag tacaaaacac agaataatgc
     8581 aatgccaaat aaaattgtac ttacttttac acaatcttat ataaaaatta ccaaatattg
     8641 agtaaaactt gaaatgctat tgttactatt gtataataaa tagaaaacaa ttcagaacct
     8701 ccgggaacaa aagctgatac gtcatgttta catgtaagca gcagattttc ctgctctcga
     8761 ggtggctctg aagtgttaca ataaaaacac atgattattt agaaaaactg gtgtttgcac
     8821 atggtatatt actgtagtat gtgcggtgag gatataaaat ggaaacaaaa attataaaaa
     8881 tagatcagga caatttagac cataagctca tgcaggaggc aggtgatttg attgcagccg
     8941 gtgagcttgt agcgtttccg acagagacag tgtatgggct tggcggtgat gctttagatc
     9001 cggaggcatc aaagaagata tacagtgcaa aggggcgtcc gtcggacaat ccgcttatcg
     9061 tacatatatc agatttttcg gatttggagc gcatcgcaaa gacagtgccg gaggatgcaa
     9121 ggaagctttc ggatgctttc tggccgggac ctcttaccat gatagtggag aagggcgatg
     9181 cagtgcctta tgccacaaca ggcggcatgg acacagtggc tgtgcgtatg ccgaatcatc
     9241 ctatagcact agatctcata agaagaagcg gctgccttat cgcggcgcca agcgccaaca
     9301 catccggcag accgagccct acagaggctg cacatgtggc tgaggatcta agcggcaaaa
     9361 tagccatgat aatagacgga ggaccggttg gcataggaat agagtccacc atcattgacc
     9421 tgacagagga tacacctatg gtactccgcc cgggatacat cacacctcag atgctttcaa
     9481 aggtattagg caaagaggtc gtcatagatc cgggaataat agcggcagat gatactagaa
     9541 agcccaaggc tcccggcatg aagtacaagc actatgcgcc aaaggctgac atggtcatcg
     9601 tggacggtac cagaaagcat gtgattgcaa agataaatga gcttgtggct tcgcacaggg
     9661 atgatggcaa aaagatagca gtcattgcga cagaggagac aaagcagttt tacgatgcag
     9721 atgttgtcct gtcgatggga agcagggcgg atgaggattc gatagcccat gaactttacc
     9781 gtatattaag ggattgtgat gagctcgatg tggatgtgat tttctcggag agcttctcca
     9841 caccgcgtat aggacaggcc atcatgaacc gtatgctaaa ggcggcaggc catcaggtga
     9901 tagacacaca tgtgaagtat gacaaaatca tttttgtcgc acagaccggc acatgcaggg
     9961 agcagatggc aaagggcatc atgaacgact ttgtactcaa ggtgccgatg gagattgagg
    10021 cgagaggact tgttgtccag tttccggagc cggtcaatca gaaggcagag gcagtgctca
    10081 taagcaatgg catttccact gagggcatgg tgtcaacgca gcttgaggaa tcggatatca
    10141 cggagagtac tatggtattt accatggagt catcacagcg cgagcgcatt atagaaagct
    10201 ttgccgatat cgacccggag caggtctttg tgctttcaca gtatgtgggc gatgagcttg
    10261 agatacttga cccttacggt ggcacgcttc aaagctacgg actgtgctat gaaagcctca
    10321 gggctacgct taagaagctg gtaaagctct taaatgcaaa tacataaaag acaaacaaat
    10381 ataacacaat ataagacaaa ggagaacaaa tattatgggt aaggtagtag ttatggatca
    10441 tccgcttatt cagcacaaga ttggaattat gagaagaacc gacacaggtt caaaggattt
    10501 cagaacactc gtcagcgagg ttgcgatgct tgagtgctac gaggcaacaa gagatctgga
    10561 gcttacagat gtggagattg agactcctat ctgtaaggcc actgtaaagg aattaaaggg
    10621 caaaaa
//
LOCUS       DPUF01000060            3679 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_2604, whole
            genome shotgun sequence.
ACCESSION   DPUF01000060 DPUF01000000
VERSION     DPUF01000060.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_2604"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            492..1751
                     /locus_tag="DHV81_02165"
     CDS             492..1751
                     /locus_tag="DHV81_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCI93330.1"
                     /translation="MIAMLLAGGQGSRLGVLTANVAKPAVSFGGKYRIIDFPLSNCIN
                     SGIDTVGVLTQYQPLRLNSHIGIGIPWDLDRNNGGVAILPPYEKSGNSEWYTGTANAI
                     YQNMRYIDSYNPDYVLILSGDHIYKMDYEVMLDFHKENNADVTIATMPVPIEEASRFG
                     IVIADDDKRINAFEEKPVHPRSNLASMGIYIFSWPVLKEALLALKDQENCDFGKHVIP
                     YCFENDRRMFAYEFNGYWKDVGTLGSYWEANMELVDLIPEFNLYEEYWKIYTKNDAIE
                     PLYIAKGGHVERSIMGEGCECYGHVEHSVIGANVKIGRGAVIRDSIIMCDTEIGSNVT
                     IDKSIIAENCKVGDNVTLGFGQEKPNVLNESIYSFGLVTIGEDSVIPDGVKIGKNTAI
                     SGVTYEEDYKDGVLEGGEVIIKAGDGK"
     gene            1748..2866
                     /gene="glgD"
                     /locus_tag="DHV81_02170"
     CDS             1748..2866
                     /gene="glgD"
                     /locus_tag="DHV81_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HCI93331.1"
                     /translation="MKAIGIILAGGNNNRMKELTAKRAVPALPVAGSFRSIDFALSSM
                     SNSHIQTVAVLTQYNARSLNEHLSSSKWWNFGRKQGGLFLFTPTVTADNSDWYRGTAD
                     AMYQNIDFLKRRHEPYVIISSGDCVYKLDFNRVLDFHIEKKSDITVVCKDMMYNDDVS
                     RFGVVRMDDESRITEFDEKPIEALSNTISTGIYVIRRRQLIELLEAAAEENRFDFVND
                     ILVRYKNVKKIYGYKINSYWNNIADIDAYFKTNMDFLRPDVRSYFFREEPTICSKVDD
                     LPPAKFNPGSDISNSLVSSGCIINGSVEDSVLFKQVFVGKNTTVKNCVILNDVYIGDN
                     VHLENCIVESRGTINPNTCYCGEDGVMIVKEKNVRYVI"
     gene            2890..3153
                     /locus_tag="DHV81_02175"
     CDS             2890..3153
                     /locus_tag="DHV81_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCI93332.1"
                     /translation="MQITDIRIRKVEKEGKMKAVVSITIDDEFVVHDIKVIEGEKGLF
                     IAMPSRKANDGEYRDIAHPINSATRENIQNMILEKYKTEIENV"
     gene            3333..3577
                     /locus_tag="DHV81_02180"
                     /pseudo
     CDS             3333..3577
                     /locus_tag="DHV81_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1208 a          656 c          892 g          923 t
ORIGIN      
        1 ctgagaacaa aaagccgatg cgtcatgatt gcatgtaagc aacagatttt cttgttctga
       61 atagttactt tatattaatt atacaactaa atatagtatt tgcaaaggaa aaataaggtg
      121 aaagcaggca tgactttcaa atgataagaa ccatagcagg ttaaacagat atacgctaaa
      181 tgacacttgt gcaaaatgtc gaaaataaag aaaaatatta tatgattttg tgaaaaatgt
      241 tgaagtgata atgcggctgt ggtataattt tttttataaa aataattcat ccggaccata
      301 tgaatttgat gggtttaaag tatatagcgg taactgcaat ttatgcagca gcaactataa
      361 tttatatgga aacaattgtg atttattatg gaagcattac agatacggtt attacagaaa
      421 caagtggtgg ggaccgggtg gcacaacaaa aattaggaca gacgaggagt gataacgtga
      481 ttaagaaaga aatgatagcc atgcttttag caggcggtca aggcagcaga cttggtgttc
      541 tgacagcgaa cgtagcaaaa ccggcagttt cgtttggtgg aaagtacaga atcatagatt
      601 ttccgcttag caactgcatc aattcaggca ttgatacagt gggtgtactt acacagtatc
      661 agccactcag acttaacagc cacatcggaa taggtattcc gtgggatctc gacaggaata
      721 atggaggagt ggcaatactg cctccttatg agaagagcgg caacagcgag tggtacacag
      781 gtacagccaa tgcaatctac cagaatatga ggtatataga cagttacaac ccggactatg
      841 tgttgatact gtcaggagac catatttaca agatggacta tgaggtgatg cttgatttcc
      901 acaaggaaaa caatgccgat gtgaccatag caaccatgcc ggtgcctatc gaggaggcca
      961 gccgatttgg tatcgtcata gccgatgatg ataagcgcat caatgcattt gaggaaaagc
     1021 cggttcatcc gcgcagtaat ctcgcatcaa tgggcattta catattctca tggccggtgc
     1081 taaaggaagc attacttgca ctaaaggatc aggaaaactg tgattttggc aagcatgtca
     1141 taccatactg ctttgaaaac gacagacgta tgtttgcata cgagttcaac ggatactgga
     1201 aggacgtagg tacactgggc tcatactggg aggccaatat ggagcttgtg gacctcattc
     1261 cggagtttaa tctgtacgag gagtattgga agatttacac aaagaatgat gcaatcgagc
     1321 cactttatat agccaaagga ggtcatgtgg agcgaagcat catgggtgag ggctgtgaat
     1381 gctatggtca tgtggagcat tctgttatag gtgccaacgt caagattgga agaggcgcag
     1441 taatacgtga ttccatcatc atgtgtgaca cggagatagg ctccaatgtc acgatagaca
     1501 agtccatcat tgccgaaaat tgcaaggtgg gcgacaatgt gacacttggc ttcggacagg
     1561 agaagccgaa cgtattgaat gagagcatat actcatttgg gctggtcact ataggagaag
     1621 attctgtgat accggacggg gtaaagatag gcaaaaatac agcaatctcc ggtgttacat
     1681 acgaagagga ttacaaagac ggtgtgcttg agggcggaga agttattatc aaggcaggtg
     1741 acggaaaatg aaggcaatag gaataatttt ggccggcggc aacaataata ggatgaagga
     1801 actgacagca aaacgagcag taccggccct gccggtagca ggcagcttca gaagcataga
     1861 ttttgcgctc agtagcatgt caaactcaca tatacagact gtcgcagtac tgacccagta
     1921 caatgcacgc tcactgaatg agcatttaag ctcatcaaaa tggtggaatt ttggcagaaa
     1981 gcaggggggc ttattcctct ttacacctac agtcacagcg gacaacagtg actggtatag
     2041 gggcacggca gatgcgatgt atcagaacat tgattttctc aagagacgcc atgagccata
     2101 tgtcatcatc agcagcggtg actgtgtata caagctggat ttcaacagag tgcttgattt
     2161 tcacatcgag aaaaaatcag acattacagt ggtatgcaag gacatgatgt acaacgacga
     2221 tgtcagccgt tttggtgtgg tacgcatgga tgatgagtca agaatcacag agtttgatga
     2281 aaagccaatc gaggccttat ccaacaccat ttccacaggt atctatgtca tcaggagacg
     2341 acagctcata gagcttttgg aggcggcagc tgaggaaaac agattcgatt tcgtaaatga
     2401 tatcctcgtg cgctacaaaa atgtaaagaa aatatatggc tacaagataa attcatactg
     2461 gaacaatatc gccgatattg atgcatactt caagaccaat atggatttct taagacctga
     2521 cgtcagaagc tatttcttca gggaggagcc gaccatctgc tcaaaggtgg acgacctgcc
     2581 accggccaag tttaatccgg gctcagacat ttcaaacagc ctcgtatcaa gtggctgtat
     2641 cataaacgga agcgtggagg attcggtgct tttcaagcag gtatttgtgg gcaaaaacac
     2701 cacagtgaag aactgcgtga tactcaatga cgtctacata ggcgacaatg tgcacctcga
     2761 aaattgcatc gtagagagcc gtggaaccat caatccaaac acctgctatt gtggtgagga
     2821 tggagtcatg atagtaaaag aaaaaaatgt aagatatgta atttaaaaac agaccagggg
     2881 gacaaaaata tgcagattac agacatcaga attcgtaagg tagaaaaaga gggcaagatg
     2941 aaggcggttg tgtcaatcac aatcgatgat gaattcgtgg tacatgacat caaggtaatc
     3001 gaaggagaaa agggactttt catcgccatg ccaagccgca aggcaaacga tggcgagtac
     3061 agggatatag cccatccgat taactctgcc acacgcgaga atatccagaa tatgattctt
     3121 gaaaagtata agacagagat tgagaatgta taaaacagtc aaatgttgta gcaaatgtaa
     3181 ataataattt tggtatgtgt atatcagacg aaatcgcgca agtgcatcga aggaaattgg
     3241 cttgcgcggt ttttgtgtaa taagggtttg attagagaat aaaaagtaag catatattca
     3301 ttttagaccc tcgtctttta gagagaaatt tgatgacaaa ctgtcctctc tgtgctacaa
     3361 tcaactcaaa gcattatatt tctgtaaata cgacaccaca gcatatttta tcggtgcgta
     3421 tcactttgaa gagggcagac cacttcgcat ttttcgacaa agacatggaa attttcaatg
     3481 ctttatgtta ccagttaata gcaggaaaat tgaaatgtca gatgtgaaaa cattgcgaga
     3541 aaagcggggt gtggggattt tgccggcttt tatataagtg attattgtac acattatata
     3601 cgtggaatgc ataagtatac agtattttta ttcgacttca gattttcctg ctcacacaag
     3661 aagaggaggt taatttggg
//
LOCUS       DPUF01000061            5360 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6902, whole
            genome shotgun sequence.
ACCESSION   DPUF01000061 DPUF01000000
VERSION     DPUF01000061.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6902"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(106..684)
                     /locus_tag="DHV81_02185"
     CDS             complement(106..684)
                     /locus_tag="DHV81_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase XerD"
                     /protein_id="HCI93333.1"
                     /translation="MNKSQPIKNKEKLDRFKNYYIEEEYNPRNYMFISLGLNTALRVS
                     DLLKFTWNDVYNFDNGCFRTHVKLTEQKTTKQSVIFLNSRIINSLSWYKSKALIKFLP
                     DIFLFSNDNNQHISRSTAYRIVHNAAVSCEIEGVISPHSLRKTFGYYAWKQGTSPVLL
                     MDIYQHSSFEITKRYLGIEQDERDSVFRDVVI"
     gene            1060..2304
                     /locus_tag="DHV81_02190"
     CDS             1060..2304
                     /locus_tag="DHV81_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HCI93334.1"
                     /translation="MVVQHNMQAANANRMLNVTTSAQSKSTEKLSSGYKINRAADDAA
                     GLTISEKMRKQIKGLDRASTNAQDGVSAVQTAEGALTEVHSMLQRMNELAVQSANGTN
                     SATDRKAIQDEVDQLTTEIDRVSETTKFNETYLLKGDSKTSDKANFMKSDYNFAANTL
                     YLEGNKTALNVDAAGKAALKKALNEGKKVYTQPYSDADNKQTADNIATKGKKYAYVTK
                     LYDADGNVVSADEIKNKKKADGTAITGNLYTSDAGKTGNDNGTNMAITIDDAAAFTQE
                     YEVNGKLSFNLHVGADSDSNNKISVDIDSMDSKGIGVKNLKVDTEDDATAAIDRISEA
                     ISKVSSQRSSLGAVQNRLEHTINNLDNVSENTTSAESRIRDTDMAKEMVNYSKNNILA
                     QAGQSMLAQANQSNQGVLSLLQ"
     gene            complement(2380..2958)
                     /locus_tag="DHV81_02195"
     CDS             complement(2380..2958)
                     /locus_tag="DHV81_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93335.1"
                     /translation="MNESNKVSRAIYVVPVIYVLGMYMMPVLFWMVCTMQESTDSFDA
                     GWIMVMPIVLGLINLAVVLILGKRISRVQLLICTRIIKYALIPFYVVGGFCIIVALLL
                     MLTPVVIMIFVGPTVAIVLSVIGWLGLLGAAPFSIAYIVRACSEGVQGKVLSVFAGIF
                     QFFFTLDVIFVIVLAVKDRSFVKAKKGYGLIQ"
     gene            complement(3009..4730)
                     /locus_tag="DHV81_02200"
     CDS             complement(3009..4730)
                     /locus_tag="DHV81_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93336.1"
                     /translation="MANTLKLPVGIDDFRKLRESHFYYVDKTRLIEQLLLNWSEVTLF
                     TRPRRFGKTLNMSMLKSFFDIGTDKALFDGLYISGNKELCDEYMGKYPVIFLSLKGVE
                     GSSFDDARYMITELIGKEAERFRFLECSEALSENDKDRYRAFISQCDGKYTMDAQLLV
                     SSLQSLSQLLCTHYGKKTIILIDEYDVPLDKAFQNGYYKEMVSLIRGLFGQALKTNEF
                     LQFAVLTGCLRISKESIFTGLNNFKVMSITDSRFDEQFGFTDSEVKKLLSDYGMDSHF
                     DEVKEWYDGYHFGKADVYCPWDVINHVDHLRDDGDAKPQTYWINSSGNSLVRRLINRA
                     DSSTKDEIERLIAGEAIEKVIRLDLTYDEIDNTIDNIWSVLFTTGYLTKIGEVKLPDS
                     ESYAYRLVIPNKEVREVFVLQIQEWFKAVVANDNDTMKLLSKAILDKDETILARQLNI
                     VMGRMISILDTKAPDDMKENFYHGLLLGLLRGSNPDWLIKSNRESGDGFSDILIKPEN
                     PDLGIVIEVKYAKEFKGLDAACDAAMAQIKQKRYDETLRDEGRCDILAYGIAFCRKRC
                     KVAGEKL"
     gene            complement(4815..5189)
                     /locus_tag="DHV81_02205"
     CDS             complement(4815..5189)
                     /locus_tag="DHV81_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93337.1"
                     /translation="MKKKLITIVIGAMLCAGLFAGCGQSDTKQDKAAGTESAKQAETK
                     DEGDKGSQEEKELTGTIDDIKDFMFVVTDDKDTPYSFTFDEKPDGLEGVASGDKVTVK
                     YTGTISEVDPFEGKIISVEKAE"
BASE COUNT         1729 a         1183 c          891 g         1557 t
ORIGIN      
        1 taaaatccat tttatccggc atgttatgaa attttcacta tgagcatgtg cctaggttca
       61 catgtccatg tatggtgaaa cacaatggat aatgtgtttc atcaactaga tgacaacatc
      121 cctaaacaca gaatcgcgtt catcctgctc gattcccagg tatcgttttg tgatttcaaa
      181 tgacgagtgc tgatatatgt ccatcaaaag aaccggtgat gtgccttgct tccatgcata
      241 gtagccaaag gttttgcgaa gggagtgtgg actgattaca ccctcaattt cacaggaaac
      301 agctgcatta tgcacaattc ggtatgctgt tgaacgggat atatgctgat tattgtcgtt
      361 tgagaacaaa aatatgtcag gtaaaaattt aattagcgcc tttgatttgt accagctcag
      421 agaattgatt atccttgaat tcagaaaaat aacagattgc ttggttgtct tttgctcggt
      481 caatttaaca tgagtacgaa aacagccatt atcaaaattg tagacatcat tccatgtgaa
      541 tttcagcaaa tccgacaccc gaagcgcggt attgagccct aatgatataa acatatagtt
      601 tctgggatta tattcctctt caatatagta atttttaaat ctatcaagct tctctttgtt
      661 tttaatgggt tgtgatttat tcattgcaat ctcctttgtg taaaatacta tggtttttgt
      721 tccatattac gacaaaaatg aattgcaatt tggatagatt aggcaatatt atgaatcaaa
      781 aacaaaaact aaatgctcga aaacactggg tttttacttg gttttaaaag aaaccagaaa
      841 taaattttaa aaaatagtac aaaatttcta aaaaggggtt aagcatttta aatctgtgcc
      901 gaaatatatg atgtaaacgg aatgaaacac attatccatt gtgtttcacc atatatgggc
      961 ttgtgaacct aggcacgtgc tcatagtgaa aatttcataa catgccggat aaaatggatt
     1021 ttatccggaa caaaataaca atacatggag gtacatatta tggtagtaca acacaatatg
     1081 caggcagcaa atgccaacag aatgctcaac gtaacaacaa gcgcacagtc aaagagcaca
     1141 gagaagcttt catcaggtta caagattaac cgtgcagcag atgatgcagc aggacttaca
     1201 atatctgaga agatgagaaa gcagattaaa ggacttgaca gagcttcaac aaatgctcag
     1261 gacggtgttt cagcagtaca gacagcagag ggtgctctta cagaggttca ctcaatgctt
     1321 cagcgtatga acgagcttgc agttcagtct gctaatggta caaattcagc aacagataga
     1381 aaagcaattc aggatgaagt tgaccagctt acaacagaaa tagatcgtgt ttctgagaca
     1441 acaaaattta atgaaacata tcttcttaag ggagatagca aaacatcaga taaggccaat
     1501 tttatgaaat ctgattataa ctttgccgct aatacattat atcttgaagg taataagact
     1561 gcattaaatg tagatgccgc tggtaaagct gcattaaaaa aagcactgaa tgaaggcaaa
     1621 aaggtataca cacagccata ctctgacgct gataataaac agacagctga taatatagca
     1681 acaaaaggta aaaaatacgc atatgtaaca aagttatatg atgccgatgg taatgttgta
     1741 tcagcagatg aaattaaaaa taagaaaaaa gctgatggaa cagctataac gggtaattta
     1801 tatacaagtg atgcaggaaa aactggaaac gataacggta cgaatatggc aattactata
     1861 gatgatgcag ctgctttcac acaagaatat gaagtaaatg gaaaattgtc attcaatctt
     1921 catgtagggg ctgattcgga ttcaaacaat aagatttctg tagatattga ttctatggac
     1981 tcaaaaggaa ttggtgttaa aaatctcaag gttgatacag aagatgatgc aacagcagca
     2041 attgaccgta tttcagaggc tatctcaaaa gtatcttcac agcgttcatc actcggtgca
     2101 gttcagaaca gacttgagca tactatcaac aaccttgata atgtaagcga gaacactaca
     2161 tctgctgagt ctcgtatccg tgatacagat atggctaaag agatggttaa ctacagcaag
     2221 aacaacattc ttgctcaggc aggtcagtct atgcttgcac aggctaatca gtctaatcag
     2281 ggtgttcttt cactcttaca gtaatcatct acaattacat gttgtacaat ataaaagagc
     2341 tcgtaaagag ctcttttata ttgctttata acgcttcaat tattgaatca atccatatcc
     2401 ctttttcgct ttcacaaagc ttctatcctt caccgccagt acgatcacaa aaatcacatc
     2461 cagagtaaaa aagaactgga atatgcctgc aaatacagac agcacctttc cttgtactcc
     2521 ctcactgcat gcccttacaa tataggcaat tgaaaaaggt gctgctccta acagcccaag
     2581 ccagccaata actgatagca caatcgcaac ggtcggacct acaaatatca tgataacaac
     2641 cggtgttagc attaagagaa gtgctactat gatgcaaaac ccacctacta cataaaacgg
     2701 tattaacgca tattttataa tccttgtgca aatgagaagc tgtactctgc ttattctttt
     2761 cccaaggata agcaccacag ccagattaat cagtcccaaa acaatcggca taaccattat
     2821 ccagcctgca tcaaagctgt ctgttgattc ctgcatagtg cataccatcc aaaataaaac
     2881 cggcatcatg tacattccaa gcacatatat cacaggaact acgtaaatag ctcgtgatac
     2941 tttatttgat tcattcatta ataaatatcc tcccttttat aaacatccac attctaatct
     3001 tttagctcct acagcttctc tcccgctacc ttacatctct tcctgcaaaa agcaatgccg
     3061 taagctaaaa tatcgcacct gccctcatct cggagagtct catcatatct cttctgcttt
     3121 atctgagcca tcgcagcatc acaagctgcg tcaagcccct taaactcctt agcatacttt
     3181 acctcgataa caatgcctaa atcaggattc tcaggcttta taagtatgtc actaaagcca
     3241 tctcccgact cacgattgga cttgatgagc cagtccggat tgcttcctct aagcaatcca
     3301 agaagcaggc catgataaaa gttttccttc atatcatcag gtgcctttgt atcgagtatg
     3361 cttatcattc gccccatcac gatattgagc tgccttgcaa gaatcgtctc gtctttatct
     3421 aaaatcgcct ttgataaaag tttcatagta tcattgtcat tcgcgacaac agccttgaac
     3481 cattcctgaa tctgaagtac aaatacctcc cgcacttctt tgtttggaat gacgagcctg
     3541 taagcgtagc tttcgctatc cggaagcttt acttccccga ttttggtaag gtagcctgta
     3601 gtaaaaagca cactccatat attgtcaatt gtattatcaa tttcatcgta tgtcaggtcc
     3661 agacgaatga ctttctcaat agcctcacct gcaatcaagc gctcaatctc atcctttgtc
     3721 gatgaatcag ccctgttgat aagacgcctc acaagactgt ttccgctact gtttatccaa
     3781 tatgtctgag gctttgcatc accgtcatca cgcagatgat ccacatgatt aatcacatcc
     3841 cacggacagt agacatcagc ctttccgaag tgatatccgt cataccactc ctttacctcg
     3901 tcaaaatgcg aatccatacc ataatcagac aaaagcttct ttacctcact atcggtaaat
     3961 ccgaactgct catcaaaacg cgaatctgta atcgacatta ccttgaaatt gttaagtcct
     4021 gtaaatatgc tttcctttga tattcgaagg caccctgtca gcaccgcaaa ctgcaaaaat
     4081 tcattagtct ttagtgcctg cccgaaaaga cctctgataa gcgaaaccat ttccttataa
     4141 tagccattct ggaaggcctt atccagtgga acatcatact cgtctataag gataatagtc
     4201 ttctttccat agtgtgtaca caataactgt gacagactct gaagactcga tacaagaagc
     4261 tgcgcgtcca tcgtatattt tccatcgcac tgcgaaatga aagccctata cctatcctta
     4321 tcgttttcag aaagtgcctc actacactcc aaaaacctga atctttctgc ttccttccca
     4381 ataagctcag taatcatgta tcttgcatca tcaaaggaac tcccttcaac acccttcaat
     4441 gacaggaaaa taaccggata ctttcccata tactcatcac acagttcttt attacctgaa
     4501 atatataagc catcaaataa agctttatct gtgccaatat caaaaaagct cttaagcatg
     4561 ctcatattaa gagtttttcc aaagcggcgc ggacgtgtaa aaagtgtcac ttcactccag
     4621 tttaataaaa gctgctcaat aagtcttgtt ttatccacat aataaaaatg cgattcccgc
     4681 aattttctaa aatcatcaat tccaaccggt aacttcaaag tatttgccat aaaagcctcc
     4741 acaaaaataa tccactatac ttagctatag tatagcggat taacattcaa aatacaatct
     4801 aagattatgg cagcttattc tgccttctca acagatatta tctttccctc aaacggatct
     4861 acctctgaaa tagtacctgt atacttaaca gtcaccttat ctccacttgc aacaccctca
     4921 agtccgtcag gcttctcatc gaatgtaaag ctgtacggtg tatccttatc atcagtcact
     4981 acaaacatga agtcctttat gtcatcaatc gttcctgtaa gctccttctc ttcctgtgag
     5041 cccttatcac cttcatcctt ggtctcagcc tgctttgcac tttcggtgcc tgctgcctta
     5101 tcctgctttg tatcgctctg tccgcagcct gcaaacaagc cggcgcaaag catagctcct
     5161 attacaattg taatcagttt tttcttcatt atgatatacc tctttcttta tttataagca
     5221 ctgctatgag tacgttttct aaagagtact cttctatcca cctaaagtaa atagaataca
     5281 taaaatatta aaaaaatctt acgaattact gcttttttga gcctcttgcg agccttgcag
     5341 gataccattt ttcaaaccat
//
LOCUS       DPUF01000062            2586 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13048, whole
            genome shotgun sequence.
ACCESSION   DPUF01000062 DPUF01000000
VERSION     DPUF01000062.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13048"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="DHV81_02210"
     CDS             complement(<1..413)
                     /locus_tag="DHV81_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCI93338.1"
                     /translation="MGKKEAKTNAIRILETMKIPYEARTYECDDFVDAAQIADKLGLD
                     HAGMYKTITTVGKSGGYYVFVVPIDDEIDFKKAAKAAGEKSIEMLHLKDLTKVTGYIR
                     GGCTSIGMKKQYPTFIHEAAKEQDKITVSGGRLGL"
     gene            complement(476..>1747)
                     /gene="ppk1"
                     /locus_tag="DHV81_02215"
     CDS             complement(476..>1747)
                     /gene="ppk1"
                     /locus_tag="DHV81_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCI93339.1"
                     /translation="RKAIDELSSFLEIGKKHFISVKTPLDMSFVFQLQHYLRDKQELF
                     FEKRTPRDTPELSLKESILGQVEKKDVLLSYPFESMKPFIKMLNDAAEDPDVVSIKMT
                     LYRVADKSQIIDALIEAAENGKEVIVLVELRARFDEANNIEMSHRLEDAGCQILYGLG
                     DYKVHSKLCLITKKKGDGFEYITQIGTGNYNEKTSRLYTDLSLITANQEIGADASRVF
                     MALQRGETVSDGDVKHLLVAPKCLQNKIVDMIDEQIANNNAGKPAYIGVKVNSLTDKV
                     LIDKLIDASQAGVKVELIVRGICCVKPQVEGATENITVISVVGRYLEHSRIYRFGVGD
                     DEKIYIASADFMTRNTVRRVEVATPVYDAHAKDKIRHIFDTIMTDDEKGKELCADGEY
                     VDRKINSTPIDSQEQFFEEAYKNADKSADNQ"
     assembly_gap    1748..1953
                     /estimated_length=206
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1954..2555)
                     /locus_tag="DHV81_02220"
     CDS             complement(<1954..2555)
                     /locus_tag="DHV81_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCI93340.1"
                     /translation="MKKLEEIYTNRELSWLQFNERVLNEAGNPRVPLAERLTFASIYQ
                     TNLDEFFMVRVGSLMMQMNSKEKIFENKTKMSSEEQVSAILDRVCELEKKKARIYEQL
                     MGELEPKGVRIINFNKLSKDEGDLLEAYFDAHIAPFLSPMIIGKQQPFPFLANKQLYA
                     VVLLTTQKGKKKTGIVPCSNSVFKRLIEIPTRPGTFMLSE"
BASE COUNT          555 a          608 c          441 g          776 t
ORIGIN      
        1 tgaagcccaa gcctgcctcc gctgaccgtg attttgtcct gctccttagc tgcctcatgg
       61 ataaatgtag gatactgttt tttcattccg attgatgtac agccgcccct tatatagcct
      121 gtaacctttg tcaaatcctt tagatgcagc atttctatgg acttctcacc tgctgccttt
      181 gcggcttttt tgaaatcaat ttcgtcatct atcggtacta caaacacata gtatccgccg
      241 cttttgccga ctgtcgtgat agtcttgtac atgcctgcgt ggtcaagacc cagcttgtct
      301 gctatctgag ctgcatccac aaagtcatcg cactcgtatg tgcgtgcctc atatggaatc
      361 ttcattgttt ccagtattct tattgcattt gtctttgctt ctttttttcc cattatacta
      421 tctttcctcc aataagctta agatatattc catatcttta tgcaattacc tcattttact
      481 gattgtccgc actcttatcg gcatttttgt atgcttcctc aaagaactgc tcctgcgaat
      541 ctatcggtgt gctgtttatc tttctgtcca catactcacc atcagcgcaa agctccttgc
      601 ccttctcgtc atctgtcatg attgtatcga agatatggcg gatcttgtcc ttagcatgtg
      661 catcgtatac cggtgttgca acctccacac gtcttacggt atttctggtc ataaaatctg
      721 ctgacgcaat gtaaatcttc tcatcatcgc ccacaccaaa tctgtatata cgtgaatgct
      781 caagatatct tcctacaaca cttattacag tgatattttc cgtggcaccc tcaacctgcg
      841 gcttcacaca gcagattcct ctgacaataa gctctacctt cacgcctgcc tgtgatgcat
      901 cgataagctt gtctataagc accttatcag tgagagagtt aaccttaact ccgatgtatg
      961 ccggttttcc tgcattatta ttggcaatct gctcatcaat catgtcaaca atcttattct
     1021 gcaggcattt tggggcaaca agcagatgtt ttacatctcc gtccgaaaca gtctcacctc
     1081 tctgaagcgc cataaacacc cttgatgcat ctgctccaat ctcctgattt gcagtgatga
     1141 gggataaatc tgtataaagg cgcgaggtct tctcgttgta gtttcctgta ccaatctgag
     1201 tgatgtactc aaagccatcg ccctttttct ttgtgatgag gcagagcttt gagtggacct
     1261 tgtaatcacc gagtccgtaa agtatctgac agcctgcatc ctcaaggcgg tgtgacatct
     1321 ctatgttgtt agcctcatca aacctcgcac gcagctcgac aagcacgata acttccttgc
     1381 cgttttctgc agcctctata agtgcatcga taatctggct cttatctgcc actctgtata
     1441 gtgtcatctt gatggaaacc acatctggat cctctgctgc atcgttaagc atcttgataa
     1501 acggcttcat gctctcaaac ggatatgaaa gaagcacatc ctttttctca acctgaccga
     1561 gtatactctc cttaagcgaa agctcaggtg tgtcacgcgg tgtacgcttc tcaaagaaaa
     1621 gctcctgctt gtctcgcaga tagtgctgaa gctggaatac gaatgacata tcaagcggtg
     1681 tttttacact gataaaatgc tttttgccaa tctcaaggaa gctcgagagt tcgtcaatcg
     1741 ccttgcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcctctg acagcataaa tgtgcccggt
     1981 cttgtaggta tctcaatcag acgcttgaat acgctgtttg agcatggcac gatacctgtc
     2041 tttttctttc ccttctgcgt ggttaaaagc actaccgcat agagctgttt attggcaagg
     2101 aatggaaacg gctgctgctt tcctataatc attggtgaga ggaaaggtgc aatgtgtgca
     2161 tcaaaatatg cctccagtaa atcaccctca tcctttgaca gcttattgaa attgattatg
     2221 cgcacgccct ttggctcaag ctcacccatg agctgctcgt aaatccttgc ttttttcttc
     2281 tcaagctcgc atactctgtc aaggattgcc gatacctgtt cctcactcga catctttgtc
     2341 ttgttctcga aaatcttttc ctttgaattc atctgcatca tgagtgatcc tactcttacc
     2401 atgaaaaact catccagatt tgtctggtag attgatgcaa aggtcagtct ctctgcaagc
     2461 ggtactcttg gattgcccgc ctcatttaat actcgctcat tgaactgtaa ccatgacagc
     2521 tccctgtttg tgtaaatttc ttccagtttt ttcatctgca tatatgctcc tattctatat
     2581 gatatt
//
LOCUS       DPUF01000063            5385 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12543, whole
            genome shotgun sequence.
ACCESSION   DPUF01000063 DPUF01000000
VERSION     DPUF01000063.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12543"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="DHV81_02225"
     CDS             complement(<1..920)
                     /locus_tag="DHV81_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCI93341.1"
                     /translation="MKKKKTLSTQLTFVISFIVLLIVGVFWMANNTLLEKYYVYNKEK
                     EMLAVYKMVDLAAKNDKLSDDTFAVAFEKKCVGANIDAVVFNKYGKVIIEGTTDNQEI
                     FQLLLEASMSSYSADYNNLNMTRQKDKRLGTEYIIINNRLIDGNFVYMKTPLESIKES
                     VSISNRFFAIGTVAAIVVGVFMAYIVARNMTRPIRNMTELSTRMAKLDFDAKYIDTEN
                     SAKELYVLGEHMNELSQTLEQTITRLKVANNELQRDIKKKEEIDEMRKEFLSNVSHEL
                     KTPLALISGYAEGLKEVVNDDDESRNFYCDV"
     gene            complement(965..>1414)
                     /locus_tag="DHV81_02230"
     CDS             complement(965..>1414)
                     /locus_tag="DHV81_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93342.1"
                     /translation="IIMLTAKGDERDELNGFDIGADEYISKPFSPKILVARVNALLRR
                     ANKLGKEAVISEAGGIVMDKTAHTVAIDGRNIELSVKEFELLDYFMSNQGIALTRERI
                     LDSVWSYDYFGDARTIDTHVKKLRSKMGAKGDYIKTVWGVGYKFEIQ"
     assembly_gap    1415..1542
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1543..1640)
                     /locus_tag="DHV81_02235"
     CDS             complement(<1543..1640)
                     /locus_tag="DHV81_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93343.1"
                     /translation="MDKIKILVVDDESRMRKLVHDFLAREGYEVLEA"
     gene            complement(1628..2167)
                     /locus_tag="DHV81_02240"
     CDS             complement(1628..2167)
                     /locus_tag="DHV81_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93344.1"
                     /translation="MYVVDGFEFQTKEEARQAKKESDGIRYIKDKTDMSDPDTVYKLY
                     LRLNKPGYFFTPVGLSFLVELQEYLHTIPYIKNEDIRPIYIPEDKKAVVEVKKQTEGY
                     KKKFHVALFFTIVLSAAIAVMFGITYVSGHSPYVVDYENELIDKYENWQKELEKREKA
                     VEEREKALDERQDATEWTK"
     gene            complement(2182..4155)
                     /locus_tag="DHV81_02245"
     CDS             complement(2182..4155)
                     /locus_tag="DHV81_02245"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742313.1"
                     /note="class 3; catalyzes the formation of fructose
                     6-phosphate from fructose-1,6-bisphosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 3 fructose-bisphosphatase"
                     /protein_id="HCI93345.1"
                     /translation="MNDIAIDKEHLHYLEQLSEMYPTIGKASSEIINLQAILNLPKGT
                     EHFVSDVHGEYEAFSHVLKNGSGAVRKKIDDVFGLAMDKADKAALATLIYYPREKMEL
                     IKQDEPDMDSWYKTTLYKLIEVCKTVSSKYTRSKVRKALPEEYAYVIEELITEKPEVL
                     NKEAYYESIINTTVELGTAEEFIVVLSELIQRLAVDHLHVLGDIYDRGAGPHLIMDRL
                     CRYHSLDIQWGNHDIIWMGAAAGNPACIATVVRNSIRYGNLDVVEEGYGINMLPLATF
                     ALKAYKDDPCTRFVFKVAPSGADNMESDLIKKMHKAIAIIRFKLEGQLIKKWPEYGMK
                     ERLLLEHIDYEQGTIELEGRTYKLLDTSFPTIDPADPYRLTEGEEEVIQRLRSSFIHC
                     EKLKNHVGLLLKRGSMYKVYNGNLLFHGCIPMEEDGSFAKVNIYGKEYSGKALFDILE
                     TYVRKAFFSDDRLERQKGSDIMWYIWTAPYSPLYGRNKMATFERYFIDDDDMKIEKKN
                     FYYDYINKPECAQRILEEFGLHDKRDHIINGHVPVHRLRGESPVKCDGRVIVIDGGFS
                     KTYRRRTGIAGYTLIYNSYGLTLTAHEPFESPETAVRDERDIVSRREAVEVLDKRILV
                     GDTDAGIKMKEKIADLKHLIAAYRSGEIAERDD"
     gene            complement(4162..4407)
                     /locus_tag="DHV81_02250"
     CDS             complement(4162..4407)
                     /locus_tag="DHV81_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93346.1"
                     /translation="MALTDYPVVSDKYYKKVYENIATDPQTGESILVQLTLQGVLDKC
                     EGTNFEEPIRKCIMKCVYTGCKLEKEINKVMNQYYEV"
     gene            4778..>5385
                     /locus_tag="DHV81_02255"
     CDS             4778..>5385
                     /locus_tag="DHV81_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCI93347.1"
                     /translation="MNRLEIVNNISTNIERERIKLGMSQAQFAKALDMSLSTYKRIAN
                     GESSRIDVYTTYLIYKLTGRFSCELTGFNDDVINLVKRIKKLSKSQRILIDSIIDTEL
                     ALSAYKDESCHTEDLISVLIPTGNMTDGMILDSYNVEKLDVSNYRKAFGDALHIGIRI
                     TSNHLHPVYNKGDILLISRNPIRDGDTGIFINAQNKRAYIRKL"
BASE COUNT         1405 a         1374 c          851 g         1627 t
ORIGIN      
        1 acatcacagt agaagttacg gctctcatca tcgtcattta cgacctcttt gagcccctca
       61 gcatagcctg agatcagagc aagcggagtt tttaactcat gtgacacatt tgacaaaaac
      121 tcctttctca tctcatcaat ctcttctttt ttctttatat cacgctgtaa ttcattgttt
      181 gccaccttta gtcttgtgat ggtctgctca agtgtctgcg acagctcatt catatgctca
      241 ccaagcacat aaagctcctt tgcactgttt tccgtatcaa tgtacttcgc atcaaaatca
      301 agcttcgcca tccttgtcga cagctcagtc atatttctta tcggcctggt catgttgcga
      361 gcaacaatat atgccataaa cactcccaca acaatagcag ccactgtgcc tattgcaaaa
      421 aagcggttcg atatggacac gctctcctta atgctctcaa gcggtgtctt catgtataca
      481 aagttaccgt caatcagtct gttatttatg atgatatact ctgtccccag ccttttatcc
      541 ttctgccttg tcatattcag gttgttgtag tctgccgaat acgacgacat gcttgcctcc
      601 aaaagcagct ggaatatttc ctgattgtca gtggttccct caatgatgac cttaccgtat
      661 ttgttgaaca caaccgcatc tatattggcg ccaacgcatt tcttttcaaa tgctacagca
      721 aacgtatcat ctgaaagttt atcatttttt gcagccaggt ccaccatttt gtataccgca
      781 agcatctctt tttccttgtt atatacataa tacttttcca gcagtgtgtt attagccatc
      841 cagaacacgc ctactatcag aagtacaatg aatgaaatca caaacgtaag ctgcgtggat
      901 agcgtttttt tctttttcat ggtgacacct cttatcccga atccattagt cctaagctta
      961 acacttactg tatctcgaac ttatatccca caccccagac agtctttata tagtcgcctt
     1021 tagcgcccat cttgcttctt aatttcttca catgtgtatc aatagtcctc gcatcgccaa
     1081 aataatcata gctccacaca ctgtcaagta tgcgctccct ggtaagagca atcccctgat
     1141 tgctcataaa gtaatccaaa agctcaaatt cctttaccga aagttcaata ttcctgccat
     1201 caatagctac agtgtgcgcc gtcttgtcca tgacaatacc accggcctcg cttattactg
     1261 cctccttgcc cagtttattc gccctgcgaa ggagagcatt taccctggcc accagtatct
     1321 ttggagaaaa tggctttgaa atgtactcat cagcaccaat gtcaaagccg tttaattcat
     1381 cacgctcatc gcccttagct gtaagcataa taatnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcctcaag tacctcgtac
     1561 ccttcccttg caaggaaatc atgcacgagc tttctcatcc tgctctcgtc atctaccacc
     1621 agtatcttta ttttgtccac tctgttgcat cctgcctttc atccagtgct ttttccctct
     1681 cttctactgc cttttccctt ttttcaagct ccttctgcca gttttcatat ttgtctatca
     1741 gctcattctc gtagtccacc acatacggac tgtgtccact cacatatgtg atgccaaaca
     1801 taacagctat ggcagccgat aatacaatag taaaaaacaa tgccacatgg aactttttct
     1861 tatagccttc cgtctgtttt tttacctcga caacagcttt tttatcctca ggaatataaa
     1921 tcggacggat atcctcattc ttgatatagg gaatcgtatg cagatattcc tgcaattcaa
     1981 caagaaacga tagccctaca ggagtaaaaa aataccccgg cttgttaagc cttaaataca
     2041 gcttgtatac cgtatccggg tcagacatat cagtcttatc ctttatatat cgtattccgt
     2101 cactttcttt tttagcctgc cttgcctctt cctttgtctg aaactcaaac ccatcaacta
     2161 catacatcat attcactctt tctaatcatc gcgctctgca atctcacccg aacgatatgc
     2221 cgcaatgaga tgctttagat cagctatctt ttccttcatc tttattccgg catccgtatc
     2281 tccgacaagt attctcttat caagtacctc tacagcctca cgccttgata cgatatccct
     2341 ctcatccctt actgccgtct ccggtgattc aaacggctca tgtgctgtca atgtcagccc
     2401 atatgaattg taaatcaggg tatatcccgc gatgcctgtc cgcctgcggt acgtctttga
     2461 aaaaccacca tctatgacta tcactctgcc gtcgcacttc acagggcttt cacctcttaa
     2521 tctgtgcaca ggaacatgtc cgtttatgat atggtctctt ttatcatgca aaccaaactc
     2581 ctcgagtata cgctgcgcac actcaggctt gtttatatag tcataataaa agtttttctt
     2641 ttctattttc atgtcatcgt catctataaa atatctctca aacgtagcca tcttatttct
     2701 gccataaagc ggagaatatg gtgctgtcca tatgtaccac atgatatcgc tgcccttttg
     2761 tctttccagc ctgtcgtccg agaaaaaagc ctttctgacg taagtctcca gtatatcaaa
     2821 caaagccttg ccactatact ctttaccgta gatatttacc tttgcgaagc ttccatcctc
     2881 ctccatcggg atgcatccgt ggaaaagcag attgccatta tataccttgt acatgctgcc
     2941 tcttttaagc agcagcccaa cgtggttttt aagcttctcg caatgaataa acgagcttct
     3001 gagcctctgt atgacttcct cttccccctc tgtcagcctg taaggatcag ccgggtcaat
     3061 tgtcggaaag cttgtatcaa gaagcttata agttctgccc tcaagctcta tagtgccctg
     3121 ttcataatca atatgttcca aaagcaatcg ttccttcatg ccatactcag gccacttctt
     3181 aatgagctgt ccctccagct tgaaacgtat gatggcaatg gccttgtgca tcttctttat
     3241 cagatcactc tccatattat ctgcgccact tggagccacc ttaaacacaa atctggtgca
     3301 tgggtcatcc ttgtaagctt tcagcgcaaa cgtagcaagc ggaagcatat ttatgccata
     3361 gccctcctcg accacgtcca gattaccata tctgatactg tttctgacaa cagttgcaat
     3421 acatgcagga tttcctgcgg ctgcccccat ccagataata tcgtgattgc cccactgtat
     3481 atccagcgag tgatatctgc acagcctgtc cattatcaga tgtggccccg ctcccctgtc
     3541 atatatatca ccaagcacat gcagatggtc aacagcaagt ctctgaataa gctcggaaag
     3601 caccactata aactcctccg cagtgcccag ctccactgtt gtattgatga ttgactcgta
     3661 gtaagcctcc ttgtttaaca cctccggctt ttctgtgatg agctcctcta taacatatgc
     3721 atactcctcc ggcaaagctt ttctcacctt ggagcgcgta tactttgatg aaacggtctt
     3781 gcacacctct atcagcttat aaagagtcgt cttataccac gaatccatat ccggctcgtc
     3841 ctgcttaata agctccatct tttcccttgg gtagtagatg agtgttgcaa gcgcagcctt
     3901 gtcagccttg tccatcgcca gaccaaatac atcatctatc ttctttctta cagccccaga
     3961 accatttttc agcacatggg aaaaggcttc atactcacca tgcacatcag atacaaagtg
     4021 ctctgtgccc tttggcaggt ttaaaatagc ctgcagattg ataatctcag atgatgcctt
     4081 gcctattgtc ggatacatct ccgacaactg ttccaaataa tgtaagtgtt ctttatcaat
     4141 tgctatatca ttcatttata tctagacctc ataatactga ttcatcacct tatttatttc
     4201 cttttcaagc ttacaacccg tgtatacaca cttcataatg cactttctga ttggctcctc
     4261 aaaattagta ccctcgcact tatccaaaac tccctgcaat gtaagctgta caagtatgct
     4321 ctcacctgtc tgcggatctg tggctatgtt ttcgtacacc ttcttgtagt acttatcact
     4381 cactaccggg tagtcagtaa gcgccatttc ccgccacttt ctaaaactta gcgacatata
     4441 ttcacctgct cttttgtata taataaaaca ttgtaccaat tagcggtgct ctaatcaatg
     4501 cctcagacac atttatttca aaaaagtttt ttgatattta ttataaataa agctgcattt
     4561 gtttaaataa aaaaagtgta agctgcattc agcttacact aattaagttt caggacacct
     4621 ccctttttat ataacaaaca ttctaatttg acagtaattg acgtttctta tatatttatt
     4681 tatgtttcaa caataaccat tatagtgtat tgcgcatgtc aatacttgac caaatttgga
     4741 gtttattttt ttaaaaagaa gggagattag ctcataaatg aaccgacttg aaatagtaaa
     4801 taacatatcg acaaatattg agcgcgaacg cataaagctt ggtatgtccc aggcacagtt
     4861 tgcaaaagca cttgatatgt cactctccac ctacaagcgg atagcaaacg gagaatcctc
     4921 tcgtatagac gtctacacca cttacctgat atacaagctg acaggacggt tttcctgcga
     4981 actaacaggc ttcaatgatg atgtaataaa ccttgtaaag cgcatcaaaa agctttccaa
     5041 aagccagcgc atacttatag actcgattat agacactgag cttgcgctat ctgcctacaa
     5101 ggatgaaagc tgtcatacgg aggatctgat ttccgtcctc atcccgacag gcaatatgac
     5161 cgacggcatg attttggact cctacaatgt cgaaaagctt gatgtttcaa attacagaaa
     5221 agccttcggt gatgcccttc acatcggaat ccgcatcaca agcaatcatc tacatcctgt
     5281 atataataaa ggcgatatac ttctaatcag tcgaaaccca atccgcgacg gcgataccgg
     5341 tatctttatc aacgcacaga acaagcgtgc atacatcaga aaact
//
LOCUS       DPUF01000064            6490 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_32957, whole
            genome shotgun sequence.
ACCESSION   DPUF01000064 DPUF01000000
VERSION     DPUF01000064.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_32957"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            1..1286
                     /locus_tag="DHV81_02260"
                     /pseudo
     CDS             1..1286
                     /locus_tag="DHV81_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742100.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase"
     assembly_gap    154..221
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1250..2737
                     /locus_tag="DHV81_02265"
     CDS             1250..2737
                     /locus_tag="DHV81_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCI93348.1"
                     /translation="MGDAGGAGGNNMNIKQAKNEVKNTVKAYLSKDAHGEYVIPQIRQ
                     RPMLLIGPPGVGKTQIMEQIARECKIGLVAYTITHHTRQSAVGLPFIKEREYDGKSYS
                     VTEYTMSEIIASVYERMEATGLKEGILFIDEINCVSETLAPTMLQFLQCKTFGNQAVP
                     KGWVIVAAGNPPEYNKSVRDFDMVTLDRVRYISIEADYQVWKEYARDVHIHDALLSYL
                     ELHPNNFYRVETDVDGMNFVTARGWEDLSSLLKVYEAGELAVTEDVIGEFIHHPDIAE
                     DVYAYLEIYRKYNEDYGISDILSGNVKKSVYKRVFDADFDERITVVNLLLSGLTVVFS
                     DVARERKMVQLWYEFLKEYRKSQRSPEEQHALYNSAVEQFSKNMEILKESSLILPKEY
                     YIRQDVLRHIKGDFDTVMDDFTEESEKLSTMEDAAGEKLNHAFDFVEDVFSDGQEMLV
                     FVTELTITPEISSFLAENECEKFDIYNEKLMVGSNRTRLLKELER"
     gene            2779..3648
                     /locus_tag="DHV81_02270"
     CDS             2779..3648
                     /locus_tag="DHV81_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct"
                     /protein_id="HCI93349.1"
                     /translation="MIYIFSAFYAEAKNIIDHYGLKKEKSPEMVRFDVFANESIRLVI
                     TGVGEINAAAAVSNIGGAYGISPDDEILNVGCGAGFSSDICLGSIFLGNKLTEQMTGR
                     TFYPDMLMKANFRECEIVTVARVLNEGCDSVVYDMEAAAVYQAAAFFVGPHRMHFIKL
                     VSDAGERIDQSKITELFALQEDKICGYIDILLSVGGNKTSIDDKTKGENMADGNATDD
                     TKSAWNMDRLICDMRCSKVMGDQLAQLIKYCRLSGIDYKAVLDEYYTNGLLPCESKRE
                     GKKCLFELKQRLL"
     gene            3663..4664
                     /locus_tag="DHV81_02275"
     CDS             3663..4664
                     /locus_tag="DHV81_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair photolyase"
                     /protein_id="HCI93350.1"
                     /translation="MCQYNKMFSHVYVEKGVAEHPRTKQILEKLNNTTVIEIDHYKDV
                     FCRKKQSISAQSNAKALILAENTSGCIYEGAPVCQSFGNENFYYCSCMMNCIFDCEYC
                     YLKGMYPSGNLVVFVNLEDIFAELEALLAQKSIYLCVSYDADLVAIESLTGFVREWIA
                     FTKKHENLTIEIRTKSGRCDLLSNYEACDRVIIAYTVSPQRIAAEYEHFASAPMERIQ
                     AAKCAMDSGFPVRLCFDPMIYCKDWRGEYSRMVGDVFSQIDGSKLWDVSIGSFRISQD
                     YLKKMRKDMPCSAVVNFPYDNVNGYYQYPENIRSDMEEFMIQAVSEYVDKDRIFMWK"
     gene            4720..5409
                     /locus_tag="DHV81_02280"
     CDS             4720..5409
                     /locus_tag="DHV81_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCI93351.1"
                     /translation="MNKAIVTGASSGIGKAICRQLAANGWLVYGIGRSFDQSDDIAGI
                     ERIVCDITDTAKLIKTIKEINKNHDISLLINNAGVGFYALHEELNPVKISQMVRTNLE
                     APMIITNLLLRDLKKNKGTIINISSITAEKTNPHGCAYGATKAGLTSFSHSLFEEARK
                     YGVRVVNLEPDMTDTNLYRNADFTVDENEAARIQPNEVADAVLYVLGQREGLVMTDIT
                     IRPQLHRIARK"
     gene            complement(5422..6360)
                     /locus_tag="DHV81_02285"
     CDS             complement(5422..6360)
                     /locus_tag="DHV81_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HCI93352.1"
                     /translation="MKFFLAPMQGLTGYTVRNAFHHNFDYIDKYYTPFIPAAKRMNAK
                     IKRDIDPVNNDGITLIPQAMSIVADEVIDLHRQLVPYGYDEINVNLGCPSGTVVSKKR
                     GSGILAYPDMLDSFLDELYTKADFPVSIKTRVGFNDDELWPKLIEIYSKYPISELTVH
                     PRVQKDFYKNTPRMDDFALAMQKINPDKTTLCYNGDITDTNSFNALTDCFPDIDEIMI
                     GRGLFANPALIAELKGISMDKAELRERLKNWHDEILSGYLSIFSGEKDAIFRMKEIWA
                     YMHGLFSDSEKLWKKIKKCQTLNDYKSIVRMAFDAF"
BASE COUNT         1961 a         1117 c         1630 g         1714 t
ORIGIN      
        1 aagctggcgc tctcggctct ttcaccaaag atgaacgagc agctttactc catggcaact
       61 gacggcacat atatttatta caatccaatc aggcttattg atatatttaa gaaaaatggc
      121 atgtatctga acagggctta tctgcacagt gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagagggat gaattcctgt
      241 ggaatacagc atgtgatata atggttgagt acacgattga tacaatggaa aagaaaagca
      301 caaagcgtat cctaagctat ataagaaaaa ccacgtatga gaggcttaaa aaagagcatg
      361 tcgtgattgc tcccggagcc ttgtacgcat ggctatacaa gcagtgtgca gaaataaagg
      421 ctgaagaaat cactgatata gatgatattg taaacacaaa tgataaaaat gagcttgcac
      481 ttctggcacg ggaattttac acagacgacc acgctttgtg gccggaggaa aaaaacgaca
      541 atgctattcc actaagttca tctgatgcca aaaagaagtg gcagaaaata tccagacaga
      601 cacgcatgga gaaaagccac tcaaaggaca gcaaatccga gggcgaacag gtaatgatga
      661 gggagctgtc cgcgaaaaga agcagacgaa gctacaagga gtttctgcgc aggtttgcag
      721 tgcttcgtga ggaggtacac gcagattatg attcctttga tatggggtat tatgcgtatg
      781 gactttcgct ttacggcaat atgccgctca ttgagcctct tgaaacgagg gagacataca
      841 agataaggga ttttgtcata gtacttgata cgagctattc tgtgagcggg gagctggtgg
      901 agcacttttt acaggagacc tttacaattc tcacggagtc ggacagcttt tttgtcagga
      961 ataaaatacg catcatacag tgtgatgaca gcgtaaagac ggacgaggaa attacagatg
     1021 aaaggcagat taagccgctt ttaaacaagt ttacgctcgt aggaggcgga ggcacagatt
     1081 tcagaccggc attttcgtat gtgagagacc ttctggataa gggagagctt aagaacatgt
     1141 gcggacttat atattttact gatggcaagg gaattttccc ggcaaagtgt ccatcgtaca
     1201 agtgcgcatt tgtgtcggtt ggtgcatatg agggtaatga ggtgccgcca tgggcgatgc
     1261 aggtggagct ggaggaaaca atatgaatat aaaacaggca aaaaacgaag tgaaaaatac
     1321 agtgaaagca tacctgtcaa aggatgcaca tggagagtac gtgataccgc agatcaggca
     1381 gagaccgatg ctcttaatcg gaccacccgg cgtaggcaag acacagatta tggagcagat
     1441 tgcaagagag tgcaaaatag gtcttgtggc atataccatc acgcatcaca cgcgccagtc
     1501 agcggtagga ctgccgttta taaaggagcg tgagtatgac ggaaagagct attccgtcac
     1561 ggaatacacg atgagtgaga ttatagccag cgtctatgaa aggatggaag caacagggct
     1621 gaaggaaggc atacttttta ttgatgagat aaactgcgtg tccgagacac ttgcgcctac
     1681 gatgcttcag tttttgcagt gcaagacatt tggtaatcag gctgttccca agggctgggt
     1741 gattgttgcg gcaggaaatc cgcctgagta caataaatca gtgcgcgatt ttgatatggt
     1801 tacgctcgac cgtgtcagat atatatcgat agaagcagac tatcaggtgt ggaaggaata
     1861 tgccagagat gtacatatcc atgatgcact tttaagctat cttgagcttc acccaaacaa
     1921 tttttacagg gtggaaactg acgtggacgg tatgaatttc gtcacggcaa gaggctggga
     1981 ggatttaagc agcctgctta aggtgtacga ggcgggagaa cttgcagtca cggaggatgt
     2041 aataggtgag tttatccacc atcctgatat agcagaggat gtgtacgcat atttggaaat
     2101 ctacagaaaa tacaatgagg attacggtat aagcgatata ttgtcaggaa acgtgaaaaa
     2161 gtcagtatat aaacgtgtgt ttgatgctga tttcgatgag agaatcactg tggtgaatct
     2221 gcttttaagc ggactgacag ttgttttttc tgatgtggcc agagagcgga aaatggtgca
     2281 gctatggtat gagtttctga aggaatatag aaagtcacaa agaagccctg aagagcagca
     2341 tgcgctgtac aacagtgctg tggaacagtt ttcaaagaat atggagatat tgaaggagag
     2401 ctctcttatt ttgccaaagg agtattacat cagacaggat gtattaaggc atataaaagg
     2461 ggactttgat acggtgatgg atgattttac cgaagaatca gagaagcttt caactatgga
     2521 ggatgcggca ggagagaagc taaaccacgc atttgatttt gtggaggatg tattctcgga
     2581 cggccaggag atgcttgtct ttgttacaga gctgaccatc acaccggaaa tcagcagctt
     2641 tttagctgag aatgagtgtg aaaaatttga tatctacaat gaaaagctca tggttggaag
     2701 caataggaca aggcttttaa aggagctgga gagataaatt ctgttgatgt atgcagggaa
     2761 gatttgaagg aattatctat gatttatata tttagcgctt tttatgctga ggcaaaaaat
     2821 ataatagacc actatggctt aaaaaaggaa aaaagccctg aaatggttag atttgatgtg
     2881 tttgcaaatg agagtatcag gcttgtgata acaggcgtag gagaaatcaa tgcggcggca
     2941 gcggtttcaa atatcggtgg tgcgtatggg atttcaccgg atgatgagat tttgaatgtt
     3001 gggtgtggcg ctggttttag cagtgatatc tgtttgggca gtatatttct gggaaacaag
     3061 ctcactgagc agatgacagg gcgtacattt taccccgata tgctgatgaa agcaaatttc
     3121 agggagtgtg aaatagtaac ggtcgcaagg gtgcttaatg aagggtgtga ttcggttgta
     3181 tatgatatgg aggcagccgc cgtttatcag gcagcagcgt ttttcgtggg accgcatcgt
     3241 atgcatttta taaagcttgt ttcagatgcc ggtgagagaa ttgaccagtc gaaaatcaca
     3301 gagctttttg ctttgcagga agacaaaata tgcggctata ttgatatact tttaagcgta
     3361 ggtggtaata aaacttcaat agatgataag actaaaggcg aaaatatggc agacggcaat
     3421 gcgacagatg atactaaatc cgcttggaat atggataggc taatttgcga tatgcgctgt
     3481 tccaaggtaa tgggggatca gctggcacag cttataaagt attgcagact aagtggtata
     3541 gattataaag ctgttctgga tgagtactat acaaatggct tgctgccgtg tgagagcaag
     3601 cgtgaaggaa agaaatgtct gtttgaattg aaacagagat tgttgtaaga aggaaatgtc
     3661 aaatgtgcca atacaataaa atgttttcac atgtatatgt ggaaaaaggc gtggccgagc
     3721 atccacgtac aaagcagatt ttagaaaagc tgaataatac aacagtcata gaaattgacc
     3781 actacaagga tgttttctgc cgcaaaaagc agagtatttc tgctcaaagc aatgccaagg
     3841 cgctcattct ggcagagaat acaagcggct gcatatatga aggtgcaccg gtgtgtcaga
     3901 gctttggaaa tgagaatttt tactattgct catgcatgat gaactgtata tttgactgcg
     3961 aatactgcta tttaaagggc atgtatccgt ctggtaatct tgtggtgttt gtaaatttgg
     4021 aggatatttt tgcggagctt gaagcacttc tggcgcaaaa aagcatatat ctttgcgtat
     4081 catatgatgc agacctcgtg gccatagagt cgcttaccgg ctttgtgagg gaatggattg
     4141 cttttacaaa gaagcatgag aatcttacaa ttgaaatccg caccaagagt ggcaggtgtg
     4201 atttgttgag caattacgaa gcctgtgaca gggtaattat tgcctataca gtttcgcctc
     4261 aaaggattgc agcagagtat gagcattttg cgtccgcacc tatggagcgg attcaggcag
     4321 caaaatgtgc tatggatagt ggatttccag tgcgactgtg ctttgatccg atgatatact
     4381 gcaaggactg gagaggtgaa tacagcagga tggttggtga cgtattttca cagatagacg
     4441 gttctaagct gtgggatgtg agtatcggaa gcttccgcat atctcaggat tatctgaaga
     4501 aaatgcgaaa ggacatgcct tgtagtgctg tggtgaattt tccatacgac aatgtgaatg
     4561 ggtattacca gtatccggaa aatatacgaa gtgatatgga agagtttatg atacaggctg
     4621 tatccgaata tgtggataag gacaggatat ttatgtggaa gtgatattat tgcaatacat
     4681 tttcaatata tatatcacat agtataaagg agcaatcaga tgaacaaagc aattgtaacc
     4741 ggggcgtctt ccggcatcgg aaaagctata tgcaggcagc ttgcagcgaa tggctggttg
     4801 gtttacggaa taggaagaag cttcgatcaa agtgatgata ttgcagggat agagcgtata
     4861 gtatgcgata ttaccgatac tgcaaagctc atcaagacaa taaaagaaat taataaaaat
     4921 catgacatat cacttctcat aaacaatgca ggcgtcggct tttacgctct tcatgaggaa
     4981 cttaatcctg taaaaatatc acagatggtg aggacaaatc ttgaggcacc gatgattatc
     5041 acaaatctgc tgcttcgcga tttaaagaaa aacaaaggaa caataataaa tatctcatca
     5101 atcactgctg aaaagacgaa tccgcacggc tgtgcctatg gtgccacaaa ggcagggctt
     5161 acgagctttt cccacagcct gtttgaggag gcgagaaagt acggtgtgcg tgtggtaaat
     5221 ttagagcctg atatgaccga taccaatctg tacaggaatg ctgattttac agtggatgaa
     5281 aatgaagctg caagaataca gccaaatgag gttgcagacg cagtgcttta tgttttgggg
     5341 caaagagaag gccttgtgat gacagatatc accataaggc cacagcttca cagaattgca
     5401 agaaagtaaa gtgtttatat tttaaaaagc atcaaatgcc attcgaacga ttgacttata
     5461 gtcattaagc gtctggcatt ttttaatttt cttccagagc ttttcagagt cagaaaacag
     5521 cccatgcata tatgcccaga tttccttcat gcggaaaatg gcatcctttt caccggaaaa
     5581 gatggacaga taaccggata aaatctcatc atgccagttc tttaagcgct cgcgaagctc
     5641 ggctttatcc atgcttatac ccttaagctc tgcgataaga gcaggatttg caaacagtcc
     5701 gcggcctatc atgatttcat caatatcagg gaagcagtcg gttagggcat taaaactatt
     5761 agtatcagtg atatctccgt tgtagcagag tgttgtttta tcaggattta ttttctgcat
     5821 ggcgagggcg aagtcatcca tgcgcggcgt atttttataa aaatccttct ggactctcgg
     5881 atgcacggta agctcactta tcggatattt actgtatatt tcgataagct tgggccatag
     5941 ctcatcatcg ttaaagccca cgcgggtttt tattgaaaca ggaaaatcag cttttgtata
     6001 cagctcatcg aggaagctat ccagcatatc aggatatgcg agtatgccgg agcctcgttt
     6061 cttggatacg acggtgcctg acggacagcc gagatttaca tttatctcat catatccgta
     6121 aggaacaagc tgcctgtgca ggtcgatgac ctcgtctgcc actatagaca tggcctgcgg
     6181 tattagagtg attccgtcgt tgtttaccgg gtctatgtct ctttttatct tggcattcat
     6241 gcgctttgcg gcagggatga atggtgtgta gtatttgtct atgtagtcaa aattgtggtg
     6301 gaatgcgttt cttactgtat atccggtcag cccttgcatc ggtgcgagaa aaaatttcat
     6361 gtgattgtga atctcctcat ttatgattaa tcagattact acatgctacg cattcgcgta
     6421 atctgactct accttttgat cctttaatca taatatggga aaaagcctga aatgtaaacc
     6481 cccataacgt
//
LOCUS       DPUF01000065            5942 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_11777, whole
            genome shotgun sequence.
ACCESSION   DPUF01000065 DPUF01000000
VERSION     DPUF01000065.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_11777"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            90..518
                     /locus_tag="DHV81_02290"
     CDS             90..518
                     /locus_tag="DHV81_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HCI93353.1"
                     /translation="MQTDETIDRYFYITGWICIAVFAALAAVIGVCGTQILTKVPPCP
                     LHAYTGYYCPGCGGTRATFALLHGHLIRSFVLHPFVPYTAVLGGWFMLSQTLQRISRG
                     RLHIGMHFRPLYMWLALAIIVVNCIVKNMLILFCGIHLLK"
     gene            complement(489..917)
                     /locus_tag="DHV81_02295"
     CDS             complement(489..917)
                     /locus_tag="DHV81_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93354.1"
                     /translation="MDPQKFDYNQDYNYNFGSNYGPGFNPNNNFYNKRSRNMEVAALV
                     LSIIAIASCSCIYVSIICGSLAMIFALLSKGGATSMSSMAMTSFWIAFAAVVITLIIY
                     IGSFAAMLHEYGSIEGILKEYQNLTGIDYNELLKQMNTAK"
     gene            1004..3364
                     /locus_tag="DHV81_02300"
     CDS             1004..3364
                     /locus_tag="DHV81_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCI93355.1"
                     /translation="MDIIAAIAEELQIKKGQAEAAVKLIDEGNTIPFIARYRKEATGS
                     LNDEVLRNLFDRLTYLRNLEDKKQTVLASIEEQGKLTDELKKAILEAQTQVAVDDLYR
                     PYRPKRRTRATIAKEKGLEPLAQTILAQESSVDIMAEAAKYVDAEKDVADEAAALAGA
                     KDIIAENVSDNAGYRTKIRELTMQKGRLISTAKDPKAESVYEMYYEFDEALSKVAGHR
                     TLAINRGEKEKVLTVKIEAPTEDIIKYLKKQVITNDGAPTAAVLTEVVEDAYDRLIAP
                     AIEREIRNNLTEEAEDGAIKVFGKNLEQLLMQPPIAGQVVLGWDPAFRTGCKISVVDP
                     TGKVLDTTVIYPTAPQNKVEEAKAVIKKLIDKHHVTLISLGNGTASRESEQVIVELLK
                     EIPVKVQYIIVNEAGASVYSASKLATEEFPNFDVGQRSATSMARRLQDPLAELVKIDP
                     KSIGVGQYQHDMNQKKLSEALGGVVEDCVNKVGVDLNTASVSLLEYISGISKTIAKNI
                     VDYREENGKFTSRSQLLNVAKLGPKAFEQCAGFMRISDGKNPLDATGVHPESYDATKA
                     VLEKLGYTMEDVKNRNVVGISKKVSDYKALADEAGVGEITLRDIVKELEKPARDPRED
                     MPKPILRSDVLEMKDLTPGMVLKGTVRNVIDFGCFVDIGVHQDGLVHISEICDRYIKH
                     PLEAVSVGDIVDVQVMSVDLKKQRIQLTMKIGQGTDKAGKSEHSGSSKDSAANKADRS
                     NRNNGGRNSRNDRNSTGSKNNNNRNKKPHYIKGKKNNFFDNIILDN"
     regulatory      3419..3533
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3664..4272
                     /locus_tag="DHV81_02305"
     CDS             3664..4272
                     /locus_tag="DHV81_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCI93356.1"
                     /translation="MDVTTNNTKSKVNIRLLVGTGMLSGLAFVLQYLEFPIPIMPGFI
                     KFDFSDLPALIGAFAYGPLAGVIVEFIKNLLHCTVTQSFTVGELSNFILGAVFTATAG
                     LIYKKNKSKKGAIIGAVVGSVVMGIVSFPSNLFIVYPAYTKFMPMEAIIGAYSELLPS
                     VGKLWQALLIFNVPFTIVKGLISTLITVLIYKRLSPVLKGRG"
     gene            4392..4889
                     /locus_tag="DHV81_02310"
     CDS             4392..4889
                     /locus_tag="DHV81_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93357.1"
                     /translation="MKLEFDVKVTEKDLYSFNIEQAYKGSQGVISLLFAVLLAAAAVL
                     SAQKGNTEYVVLYIIVGILVLLYVPFSLKGRVKMIMKTNEVFSKPLHFEIDEKNIKVS
                     QGDEMAQLPWEQVYRFVANDKRILIFSNRKNAYILSKEQLGDSYEPLKELAGSTLEKY
                     RLKIK"
     gene            4940..5389
                     /locus_tag="DHV81_02315"
     CDS             4940..5389
                     /locus_tag="DHV81_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCI93358.1"
                     /translation="MSSEGNITVIESFSADDTHALGVTLGQQAKPGDVCTLVGDLGVG
                     KTVLTQGIAEGLGITEPINSPTFTIVQVYEEGRLPFYHFDVYRIGDIEEMDEIGYEDY
                     FYGDGLTMIEWANLIEEILPKKRKEITIEKDLEKGFDYRKITIKEVL"
     gene            5389..>5637
                     /gene="tsaB"
                     /locus_tag="DHV81_02320"
     CDS             5389..>5637
                     /gene="tsaB"
                     /locus_tag="DHV81_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCI93359.1"
                     /translation="MKILALDSSGLVASVAVVSDDNLIGEYTINYKKTHSQTLLPMLD
                     EVAKMTELDLKTIDFIAVSAGPGSFTGLRIGSATAKGLA"
     assembly_gap    5638..5811
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5812..>5942
                     /locus_tag="DHV81_02325"
     CDS             <5812..>5942
                     /locus_tag="DHV81_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCI93360.1"
                     /translation="VFRDYINEHVKVDYDFAPAMCNKQRASAVACLGQVLYEQGRAE"
BASE COUNT         1840 a         1094 c         1431 g         1403 t
ORIGIN      
        1 ttaaattaat gctttgacaa ttgtcagata tatagcatat acttgtattc atatggttac
       61 acaaaatcac aggaaggacc acacatatca tgcagacaga tgaaacaatc gacaggtatt
      121 tctatataac gggctggata tgcatagctg tatttgcggc tcttgcggca gtgattggag
      181 tatgcggtac gcagatactc acaaaggtgc ctccatgtcc gctgcacgca tatacaggct
      241 actattgtcc ggggtgcggt ggcacgagag ccacatttgc gctgctgcat ggacatctga
      301 taaggtcgtt tgtactccat ccgtttgtgc cgtatacagc cgttttgggc ggctggttta
      361 tgctgtcaca gacactgcag cggataagcc gcggcaggct gcacatcgga atgcacttca
      421 ggccattata tatgtggctt gcacttgcca ttattgtggt aaactgtata gtcaaaaata
      481 tgcttatatt attttgcggt attcatctgc ttaagtagct cattgtagtc gataccggtc
      541 aggttctggt attcctttaa tataccctct atgcttccat attcgtgaag catggcggca
      601 aacgaaccga tatagataat aagagtgata acaacagcgg caaacgcaat ccagaatgag
      661 gtcatagcca tgctgctcat tgaggtggca ccgccttttg acagtaacgc aaaaatcatg
      721 gcaagcgagc cgcatatgat agacacataa atacagctgc acgacgcgat ggcaattatt
      781 gaaagcacaa gtgctgcaac ctccatattg cgtgagcgtt tattgtagaa attgttgttg
      841 ggattaaagc ccggcccata attactgccg aaattatagt tataatcctg attataatca
      901 aatttttgtg gatccataat aaaattcctt tctataaagt tgattttatt gtatcacttc
      961 atttataata cgaaaagaat aagtttttta aagaggtaaa aaaatggata ttattgctgc
     1021 aattgcagaa gaactgcaga ttaaaaaagg acaggccgag gccgctgtca agctgataga
     1081 tgagggaaac accattcctt ttatcgccag ataccgaaag gaggcaaccg gctcattaaa
     1141 tgatgaggtg ctcagaaatc tcttcgacag actgacatat ctacgtaatc ttgaggacaa
     1201 gaagcagaca gtgcttgcaa gcattgagga gcagggcaag cttacggatg agcttaagaa
     1261 ggcaatcctt gaggcacaga cacaggtggc ggttgacgat ttgtacagac cttaccgtcc
     1321 gaagagaaga acccgtgcca caatcgcaaa ggaaaagggc ttagagccac tggcacagac
     1381 cattttagcg caggaatcgt ctgttgatat catggcagaa gcagctaaat atgtggatgc
     1441 agaaaaagac gtggcagatg aagcggctgc acttgccgga gcaaaggata tcattgcaga
     1501 gaacgtatcc gacaatgccg gttacagaac aaaaatcaga gagcttacca tgcaaaaggg
     1561 gcgtcttata tcgacagcca aggatccaaa ggcagagtct gtctatgaga tgtactatga
     1621 atttgatgag gctctttcca aggtggcagg tcacagaaca ctcgcaatta accgtggtga
     1681 gaaggaaaaa gtcctcacag taaagataga ggcgccgaca gaggatatca taaaatatct
     1741 taaaaagcag gttatcacaa atgatggagc gcctacagca gccgtactta cagaggtagt
     1801 ggaggatgcg tacgacaggc ttattgcccc tgccatagag cgtgaaatca gaaacaatct
     1861 gacagaggaa gcagaggatg gagccataaa ggtattcgga aagaacttgg agcagcttct
     1921 catgcagcct ccaattgcag gacaggttgt gcttggatgg gatccggcat tccgtacagg
     1981 ctgcaagatt tctgtcgtag accctacagg aaaggtgctc gataccacag taatctatcc
     2041 gacagcacca cagaacaagg tcgaggaggc aaaggccgta atcaaaaagc tcatagacaa
     2101 gcatcatgtc actcttatct cactcggaaa cggaacagca tcgcgtgagt cagagcaggt
     2161 gattgtcgag ctgttaaagg aaattccggt aaaggtgcag tacatcatag taaatgaagc
     2221 aggtgcgtca gtatattcag ccagcaagct tgccacggag gagtttccga attttgatgt
     2281 gggacaaaga agtgccacat ccatggcgcg tagacttcag gatccgctcg ctgagcttgt
     2341 aaagattgat ccaaagtcaa tcggagtcgg acagtatcag cacgatatga accagaaaaa
     2401 gctctcagag gcactcggag gagttgtaga ggactgcgta aataaggttg gtgtagattt
     2461 aaatacagca tcagtttcac tgcttgagta catatcaggc atctcaaaga ccattgccaa
     2521 aaacatcgtg gactacagag aggaaaacgg taaattcaca tcacgttcac agcttcttaa
     2581 tgtagcaaag cttgggccga aggcatttga acagtgtgca ggcttcatgc gtatctcaga
     2641 cggaaagaac cctcttgatg ccacaggcgt gcatcctgag tcatatgatg ccacaaaggc
     2701 agtgcttgaa aagctcggct acacaatgga ggatgtaaag aacagaaatg tagttggaat
     2761 ctcaaagaag gtatctgatt acaaggcgct cgcagacgag gcgggagttg gtgaaatcac
     2821 actgcgtgac atcgtaaaag agcttgaaaa gccggcaaga gacccgcgtg aggatatgcc
     2881 aaagccaatt ctaagaagcg atgtccttga gatgaaggac ctcacaccgg gtatggtatt
     2941 aaagggaaca gtcagaaatg taatagattt cggatgcttc gtggatatag gagtacatca
     3001 ggacggactt gtgcatatct cagagatatg tgaccgctat atcaagcacc cgcttgaggc
     3061 tgtcagtgta ggagacatag tagatgtgca ggttatgagc gttgacctca aaaagcagcg
     3121 catccagctt accatgaaaa tcggacaggg cacagacaag gccggtaagt cagaacacag
     3181 cggcagcagc aaagacagtg ctgcaaacaa ggctgataga agtaatagaa ataacggtgg
     3241 caggaatagc agaaacgata gaaatagtac aggcagcaag aataataaca acagaaacaa
     3301 aaagccacac tacataaagg gcaagaaaaa taatttcttt gacaatatta ttcttgataa
     3361 ttaaaaacac cttgacaaaa cacttcaaag tgcatatcat aggtttgtaa aataaatagg
     3421 aattcttcag ggcagggtgc gattccctac cggtggtaca gcccacgagc cgcaaggcat
     3481 gattcggtgt gattccgaag ccgacagtat agtctggatg agagaagatt attaggtgtc
     3541 attacactgt gatattaagt cccggaagat tttcttccgg gattttttaa agccgctatt
     3601 cagattttag atagctgcac tcacaatatc ctcaggattc cttaagcatc aaaggagtgt
     3661 aaaatggacg taacaacaaa caacacaaaa tcaaaagtaa acatcagact tttagtcggc
     3721 acaggaatgt taagcggact tgcatttgta cttcagtacc tcgagtttcc aattccaatc
     3781 atgccgggct ttattaagtt tgatttctca gatctgcctg cacttatcgg cgcatttgca
     3841 tatggaccgt tagcaggagt gattgtagag tttatcaaga acctgcttca ttgtacagta
     3901 acacagagct ttacggtagg agagctttca aactttattc tcggagcagt atttacagca
     3961 acagcaggac ttatctacaa gaagaataag tcaaaaaagg gtgcaataat cggcgctgta
     4021 gtcggttcag tggtaatggg aatcgtcagc ttcccgtcga atctgtttat cgtgtatcct
     4081 gcatacacaa agttcatgcc aatggaagca atcattggtg cctacagtga gcttctccca
     4141 tcagtaggca agctgtggca ggcactttta atattcaatg ttccgtttac catcgtaaag
     4201 ggacttatat caacacttat cacagtgctt atatacaaga gactgtcacc ggttttaaag
     4261 ggcagaggat agagaaaata cctgatacaa tatatattag tcacaaacat aaagagcgca
     4321 gtcaattgcg ctcttttctt tttttaaaag gaatgttata ataaatccaa atatttagtt
     4381 gggagcagta tatgaagctt gaatttgatg taaaagtaac agaaaaggat ttatattcat
     4441 ttaatataga gcaggcgtac aagggcagtc agggtgtcat atcattgctg tttgctgtgc
     4501 tcttggctgc ggcagcagtg ttaagcgcac aaaaaggcaa tacggagtat gtggtgcttt
     4561 atattatagt tggtattctg gtgctgctat atgtgccgtt ttcactcaag ggcagggtaa
     4621 agatgattat gaagacaaat gaagttttct caaagccact gcattttgag atagatgaaa
     4681 agaacataaa agtaagccag ggagatgaga tggcgcagct tccgtgggag caggtgtaca
     4741 gattcgtggc aaatgataag aggatactta tattcagcaa caggaagaat gcatacattc
     4801 tctcaaagga gcagctgggc gacagctatg agccgttaaa ggagctggca ggcagtacac
     4861 tggagaaata cagactgaaa atcaaataaa caaaatagat ataagataaa ataaatagat
     4921 aagggtataa gctggtaata tgagtagtga aggaaatata acagtaatag aaagcttttc
     4981 ggcagatgat acgcatgcac ttggggttac gcttggacag caggcaaagc cgggggatgt
     5041 gtgtacactt gtgggtgatt taggagtggg taaaacagtg ctcacgcagg gcatagcaga
     5101 ggggcttggc atcacagagc cgataaacag ccctacattt accatagtgc aggtgtacga
     5161 ggagggcagg ctgccgtttt atcatttcga tgtatacagg attggtgaca ttgaggagat
     5221 ggatgaaata ggctatgagg attatttcta cggagatggg cttacgatga tagagtgggc
     5281 aaatctcatt gaggagatac tcccaaagaa gagaaaagag attactattg aaaaagacct
     5341 ggaaaaaggc tttgactata gaaaaataac aataaaagag gttttataat gaagatactt
     5401 gcgcttgaca gctctgggct tgtggccagt gtggctgtcg tatcggacga taatctgata
     5461 ggagaataca cgataaatta taaaaagaca cattcgcaga cacttcttcc gatgcttgat
     5521 gaggtggcta agatgacaga gcttgacctt aagacaatag attttattgc agtctcagca
     5581 ggacctggat cgttcacagg gcttcgtata ggctctgcga cggcgaaggg gcttgcannn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgttcagg
     5821 gattatatca atgagcacgt aaaggtggac tatgactttg cacctgccat gtgcaataag
     5881 caaagagcat cggctgtagc atgtcttgga caggtgctct atgagcaggg cagggcagaa
     5941 aa
//
LOCUS       DPUF01000066            2808 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7907, whole
            genome shotgun sequence.
ACCESSION   DPUF01000066 DPUF01000000
VERSION     DPUF01000066.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7907"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(73..1064)
                     /locus_tag="DHV81_02330"
                     /pseudo
     CDS             complement(73..1064)
                     /locus_tag="DHV81_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567886.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    154..234
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1090..>1652)
                     /locus_tag="DHV81_02335"
     CDS             complement(1090..>1652)
                     /locus_tag="DHV81_02335"
                     /inference="COORDINATES: protein motif:HMM:PF01478.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93361.1"
                     /translation="RCYNSSHERRGDENTVIVKLISHLMLYILLTVAAIGDFKNYRIS
                     NRLILTGLGCAFLFRLVGGKVASIIWFLPDIVFPVVILYFLYLSGILGAGDIKLFSVV
                     CAFTNLRFTALCMAAAFVAAAGYGLISMVLKKELFVRMRDGFLYLNGILCGRFMRYRG
                     QGKAVSFAVYLLLGTAMADIWFYGMR"
     assembly_gap    1653..1783
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1880..2092
                     /locus_tag="DHV81_02340"
     CDS             1880..2092
                     /locus_tag="DHV81_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 domain-containing protein"
                     /protein_id="HCI93362.1"
                     /translation="MKLFKETPVNDGYKTLQDDIKKTTDELQIVYTNLENVVEPDLID
                     YYIYQAKAVSMRYKFLLNCAKRLNEV"
     gene            complement(2113..2586)
                     /locus_tag="DHV81_02345"
     CDS             complement(2113..2586)
                     /locus_tag="DHV81_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93363.1"
                     /translation="MKNELKQELTRRLCECNNGGLVLVMYDIYFGFSEDVKAALASND
                     RAALKKAVADVQAALDELIGALDFKYPLAGQLYPLYSYCKRLFSTVLYSLDVSVVNEA
                     DSIMKKLRISFETAAADDKSAPLMDNAQKVYAGMTYGRGRLTESFINDDNRGFLA"
     gene            complement(2593..>2808)
                     /locus_tag="DHV81_02350"
     CDS             complement(2593..>2808)
                     /locus_tag="DHV81_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HCI93364.1"
                     /translation="IGAFQNRLESAESSLSETSENMTGAYSNIMDTDMATEMTEYAAQ
                     NILDQAGISVLSQANDLPQQVLSLLSR"
BASE COUNT          750 a          657 c          424 g          765 t
ORIGIN      
        1 atttcctcat cactcagctc tctggacaaa tccatttcag aaagtataat atctcttatt
       61 ctctgtccaa aatcatccag cacacgaaat accccgagtc atgtttctta tgacatctcc
      121 aaactcattc cacttccaca gctctacaag cttnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcctct
      241 gtaaactcgt actctctcca ctggctgatt ccgccttttt tctgtaccag gtagatatca
      301 ctgcatctgt caagaatatc aaaaaagccc ttaaatcttg ttcccatatt gagaactatg
      361 cagtcatagt gcatttcctt gccaagcatc tgaagcagat tgttgcatat taccgtattt
      421 atttcacaca gacactctgt attttcaatc ggatatacat aatccagctt atgctttctt
      481 ccgatacagc tgtttatcct gttggtcgag tatcgtccgg attcggccat caccaatagc
      541 tcgtccagat tatgctccac actctgctca agcatccgcg ataatccact attctcctga
      601 aaatccataa gcagtacctt ttcattctca ctcaaaatag aggacaaagt catcgcaaaa
      661 ggcatctgat actgattatc agcaagtgaa tacacgctta taagcctcgt ctgacctatc
      721 aaagagccct cactcttaag gtctcttacc tcaccgccca catgctttaa aatctcttcc
      781 actatgcggt ttacttcctg atatttttcc acaaagtata taccctcctc actctcaata
      841 ggtgtttcct caacaatctg tatcagtggg atatacttgt tattccactg gtcaagctca
      901 cttgaaagca tgatatctat ctcgccctct aagtgttcca gctgctcttt catggtatac
      961 atatgtatct ccagctgatc cttataatga ttcatgaaat agcatgtcag tctgtcgcca
     1021 tattctgaat cctcaatata aattcctagt ctgatttttt tcatctatgg tactttaacc
     1081 ctccttccat cacctcatgc cgtaaaacca tatatctgcc atggctgttc ccaaaagcaa
     1141 gtataccgca aaactaacag ctttcccctg tccgcggtac cgcataaatc tgccacacag
     1201 tataccgttt aagtataaaa atccatccct catacgcaca aagagctcct ttttaagcac
     1261 catacttatg agaccatatc ccgctgccgc cacaaaagcg gctgccatgc acaaggcggt
     1321 aaatcttaaa tttgtaaatg cacatactac tgaaaataac ttgatatccc cggctcctaa
     1381 aatgcctgaa agatataaga aatataaaat gacaaccgga aaaactatat ctggaagaaa
     1441 ccagatgatg ctcgctacct tgccccccac taagcgaaat agaaacgcac aaccaagtcc
     1501 cgtcagaatc agccggttac tgattctata atttttgaaa tccccaattg ctgctactgt
     1561 aagcagtata tacagcatga ggtgtgatat taatttcaca atcactgtat tttcatcacc
     1621 tctcctttcg tgagacgaat tataacaacg cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttttca catgtcaaaa
     1801 gcacttttta caaaatctgt gaatagaaga aactcttttg cttatacttg tattaagact
     1861 tattttgttg gaggaattca tgaaattatt taaagaaacg cctgtaaatg acggttacaa
     1921 gaccttacag gatgacatca agaaaacaac agatgaactt caaattgtat acactaacct
     1981 ggaaaatgtg gttgagcccg atttaattga ctactacatc tatcaggcca aggctgtttc
     2041 catgcggtat aaatttctat taaactgtgc caagcgactt aatgaggttt aatgaactaa
     2101 aaaagaggcc tcctacgcaa ggaagcccct gttatcatcg tttataaagc tctctgtgag
     2161 cctgcccctt ccataggtca tgcccgcata gaccttctgc gcattgtcca taagcggagc
     2221 cgatttgtca tcggcagctg cggtctcaaa gctaattctt agctttttca taatgctgtc
     2281 agcctcattc accactgaca catcaagtga gtaaagcact gtggaaaaaa gcctcttgca
     2341 gtatgagtac agtggataaa gctgtcctgc gagaggatac ttaaagtcaa gcgcacctat
     2401 cagctcatca agcgctgcct gtacatccgc cactgccttt ttcaaagctg ccctgtcatt
     2461 tgaagccagg gcagctttta catcttctga aaatccaaaa tatatatcat acataacaag
     2521 cacaagccca ccattgttgc actcacaaag ccttctggtg agctcctgct tcaattcatt
     2581 tttcattgct tattacctcg acaatagtga aagtacctgc tgtggcaggt catttgcctg
     2641 agacagcact gatattccgg cctggtcgag aatattctgt gctgcgtatt ccgtcatctc
     2701 ggttgccatg tcggtatcca tgatattgga atatgcaccg gtcatgttct cagaagtctc
     2761 agaaagactg ctctcagcag attccagacg gttttggaag gctcctat
//
LOCUS       DPUF01000067            8026 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_37836, whole
            genome shotgun sequence.
ACCESSION   DPUF01000067 DPUF01000000
VERSION     DPUF01000067.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_37836"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /gene="gltA"
                     /locus_tag="DHV81_02355"
     CDS             complement(<1..1385)
                     /gene="gltA"
                     /locus_tag="DHV81_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase (NADPH), homotetrameric"
                     /protein_id="HCI93365.1"
                     /translation="MEVDVLKRVPVREQDPKVRATNFEEVCYGYNKEEAMAEASRCLN
                     CKNAQCMKGCPVSINIPAFVEQVKNGDFTKAYEIISESSALPAVCGRVCPQESQCEGK
                     CIRGFKGDPVSIGKLERFVADTARENGIKPKTAAEKNGKKVAVIGSGPAGLTCAGDLA
                     KLGYDVTIFEALHAAGGVLSYGIPEFRLPKDKVVAAEVDNVKALGVKIETNVVIGKSV
                     KIDELMEEEGFEAVFIGSGAGLPRFMGIPGEQANGVFSANEFLTRNNLMKAFKEGYET
                     PISKGKKVVVVGGGNVAMDAARTALRLGAETHIVYRRSEAELPARKEEVHHAKEEGVI
                     FDLLENPTEILVDENGWVKGVKLIKMELGEPDASGRRSPIEIAGSEYEMECDTVIMSL
                     GTSPNPLISSTTIGLDTNKRKCIVATEDTGATSKEGVFAGGDAVTGAATVILAMGAGK
                     AAAKGIDEFLSA"
     gene            complement(1385..2275)
                     /locus_tag="DHV81_02360"
     CDS             complement(1385..2275)
                     /locus_tag="DHV81_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HCI93366.1"
                     /translation="MYPIVRKEKLADNIILMDIKAPRVAKECLPGQFIIAKTDEVGER
                     IPLTICDYDREKETVTIVVQTIGAGTERMMALNEGDSLEDFVGPLGCPSELCQEDNLE
                     ETKKKHIVFIAGGLGTAPVYPQVKWLKEHGVDADVIMGFRNKDILFFEDEMKAVAKNL
                     YVCTDDGSYGFHGNGSQQLQALVDAGNTYDCCVAIGPMIMMKFTCLLTKKLEIPTIVS
                     MNPIMVDGTGMCGACRLIVDGKVKFACVDGPEFDGHLVDFDQAMKRQQQYKTEEGRAK
                     LAYEEGATHHGGCGNCGGDK"
     gene            complement(2516..3460)
                     /locus_tag="DHV81_02365"
     CDS             complement(2516..3460)
                     /locus_tag="DHV81_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCI93367.1"
                     /translation="MAEEIPVYLFTGFMDSGKTSLIKDTIFENDFANGSKGLIIMCED
                     GDVEYDMDKLKAANIQVAEIDSEDEFTAAKLNELNMQYLPEQVFIEYNGTWGIDKIIE
                     AELPKGWVIVQSLATVDSTTFDLYMNNMRAMMQEQVFASDVVIFNRTDDDTDRGHLRR
                     TIKNINRKAQIVYERKDGTIDERPEELPFDINQDVIELSDADYAIWYMDAMENYKKYD
                     GKVVKFLALVYNPDKLRKGVMVPGRFAMTCCIEDVTFIGFKCKYEDESSIPHKSWITI
                     TARVHVEFAREYKGKGPVLYPVSIEPAEKPQDELVYFS"
     gene            complement(3506..4642)
                     /locus_tag="DHV81_02370"
     CDS             complement(3506..4642)
                     /locus_tag="DHV81_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HCI93368.1"
                     /translation="MTKIDIISGFLGAGKTTFIKKMIDEAFKGEQIVLIENEFGEVGI
                     DGGFLKDAGIQITEMNSGCICCSLVGDFGKNLNEVITKYHPDRILIEPSGVGKLSDVM
                     KSVIDIEKEQPVKLNALVTVVNALKASKQMKAFGEFFNNQIEYATTVILSRSQNATPE
                     QLEFCVKQIQALNPKASVITTDWDAIKGEQILKTMEGQDNLEMKVLAEARHAQDEAEH
                     HHHHDHDEHEHHHDHDEHEHHHDHDEHEHHHDHDEHEHHHDHDEHEHHHEHGEGCTCG
                     CHDHDHEHGHHHADDVFTSWGKETPHKFERAKLEEILKQFVEDDKILRSKGMVEGTDG
                     KWIYFDMVPGEYEIRDGEPDYTGRIVVIGTDIHEHELEELFGLA"
     gene            complement(4779..4955)
                     /locus_tag="DHV81_02375"
     CDS             complement(4779..4955)
                     /locus_tag="DHV81_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCI93369.1"
                     /translation="MSSVIVKENETLDSALRRFKRNCAKAGIQQEIRKREHYEKPSVK
                     RKKKSEAARKRKYN"
     assembly_gap    5082..5152
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5168..7057)
                     /locus_tag="DHV81_02380"
     CDS             complement(5168..7057)
                     /locus_tag="DHV81_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCI93370.1"
                     /translation="MKLDSKKSKAAVKNSAVKQKKVKAKAANPAKAPKVKKTLNTKKA
                     QKVNNKPKVKGKKKLSTKLILIPVFIVGFVSVISSGLSLKNLSKVNDAASQIVDTSMV
                     GTEMLNDIEMETVNIHTLALSHIISTDLSSMIDIVSEVRNHEEKLKQLLDDYNPYVTL
                     ETKRNYRIICENYTSLVKECGNVMAYSAAGKNEEAYKTANGSIAKYTNNIEKAISSMR
                     EHVNSVTQEERKSLESTYRASVVTCTFTIAVSIIALLFVVFAVVTMVIKPLLRTQKEI
                     NGIIVNIDNKKGDLTQRVTPINNAEVDAVGKGINVFMTKLQAIFKAVVTNSARMERVV
                     DDVRQSVQTSNSSVSDLSALTEELSATMQDISENASVINTNTDNVAKEVELIAEKTDE
                     LTGYTRDMKAHAQSMESVARTNMESTDRKVSEILEVLQKAIEDSNSVKQVNSLTNDIL
                     NIASQTNLLALNASIEAARAGEAGRGFAVVASEISQLAAASQEAANNIQRINAVVTNS
                     VNNLSDNANGLVSYINDSILPEFERFVESGVEYNDKASFIEGTMTDFKEKTDSLKQSM
                     LEISSSINTISHAINEGVNGVVSAADSTQLIVEDMDNISHKMDENYEIADSLKKETSI
                     FIKLD"
     gene            complement(7071..>8026)
                     /locus_tag="DHV81_02385"
     CDS             complement(7071..>8026)
                     /locus_tag="DHV81_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI93371.1"
                     /translation="IADDQITSMVAGGALYEIEDVDAVKKADDEGAVEAATIDGKLYG
                     YPLTADNGYFMYYNKNYFSDSDVATLDGMLDIAGANGKYLTMDWSSGWYLYSFFGNTG
                     LDFGVNDDGVTNHCNWNSIITDIKGVDIAQAMLDIAAKPGFKNCVQDDFIAGVQDGSI
                     IAGVSGCWNAAKIKEIWGDDYGAVKLPTYTCAGQQIQMSSFKGYKYMAVNAYTKYPEW
                     ANKLAQWFTNEDNQKLRFEMKNVGPANKAAAADDAVKKVPAIMAVMDQAQYGKLQRVG
                     NSYWDAATEFGEKMAAGNPDNTDLQEIMDNLVAGITQSVAE"
BASE COUNT         2067 a         2002 c         1394 g         2492 t
ORIGIN      
        1 gcagataaga actcgtcaat accttttgct gctgcttttc ctgcacccat ggcaagaata
       61 accgttgcag cacctgttac ggcatctcca cctgcgaata caccctcttt tgaggttgca
      121 cctgtatcct cagtagctac gatacatttt ctcttgtttg tatcaagacc tattgttgta
      181 gaagaaataa gtggatttgg agatgtacca agtgacatga tcactgtatc gcactccatc
      241 tcatactcag agccggcaat ctcaataggg cttctacgtc ctgatgcatc aggttctcca
      301 agctccatct tgataagctt aacaccctta acccagccat tctcatcaac aagaatctct
      361 gtagggttct caaggagatc aaagataact ccctcttcct tggcatgatg tacttcctct
      421 ttacgtgcag gaagctctgc ctcactacgt ctgtatacaa tgtgtgtctc tgcaccgaga
      481 cgaagagctg ttctggctgc gtccatggct acgtttccac cacctacaac tacaaccttt
      541 ttgccttttg agataggagt ctcataaccc tctttaaaag ccttcatgag gttatttctt
      601 gtaaggaact cattagcaga aaatacaccg ttggcctgct ctccagggat tcccataaat
      661 cttggcagtc ctgctcccga accgataaat acagcctcaa agccttcttc ctccataagt
      721 tcgtcaatct taactgactt tccgataact acatttgtct caattttaac accaagtgcc
      781 ttgacattat ctacttctgc cgcaacaacc ttgtcctttg gaagacgaaa ctccggtatt
      841 ccgtagctta atactccgcc ggcagcgtga agtgcctcaa aaatagttac atcatagcca
      901 agctttgcaa ggtctccggc acatgtaagt cctgcaggac ctgaaccgat aacagcaacc
      961 tttttgccgt ttttctctgc ggctgtcttt ggcttgatgc cattctctct ggctgtatca
     1021 gcaacaaatc tctcgagctt tccgattgag acaggatcgc ccttgaagcc tctgatacac
     1081 ttgccctcac actggctctc ctgtgggcat acacgtccac atacagcagg aagtgctgat
     1141 gactctgaaa taatctcata agcctttgtg aaatcaccat ttttaacctg ctcaacaaat
     1201 gcaggaatat taatagaaac agggcagccc ttcatacact gtgcattttt acagtttaag
     1261 catctgcttg cctctgccat tgcctcttct ttattgtagc cgtagcaaac ctcttcaaag
     1321 tttgtggcac gaacctttgg atcctgctct ctgacaggta ctctctttaa tacatcgact
     1381 tccattattt gtcacctccg cagtttccgc agccaccgtg atgtgttgcg ccctcttcat
     1441 aggcaagctt agctctgcct tcctcagtct tatattgctg ctgtctcttc atagcctgat
     1501 caaaatcaac aagatgtccg tcgaactcag gtccgtcaac acatgcgaac ttaacctttc
     1561 cgtccacgat cagacggcat gctccacaca tacctgttcc atcaaccatg attgggttca
     1621 ttgaaacaat tgtaggaatc tcaagcttct tcgtgagcag acatgtgaat ttcatcataa
     1681 tcataggtcc gatagctaca cagcagtcat atgtattgcc ggcatcaaca agtgcctgaa
     1741 gctgctggct gccgttgccg tggaagccgt atgagccatc atctgtacag acatagagat
     1801 tctttgcaac agctttcatc tcatcctcaa agaaaaggat atccttattt ctaaagccca
     1861 tgatgacatc agcatccaca ccatgctctt taagccactt aacctgtgga tatacaggag
     1921 ctgtaccaag acctcctgcg ataaatacga tgtgcttttt ctttgtctcc tcgaggttat
     1981 cctcctgaca aagctctgac ggacagccga gtggtccgac gaaatcctct aatgaatccc
     2041 cctcattaag agccatcatt ctctctgttc ctgcaccgat agtctgtact acgatagtaa
     2101 cagtctcttt ttccctgtca taatcacaga tggtaagtgg gattctctct cctacctcat
     2161 ctgtctttgc gatgataaac tgaccaggca gacactcttt tgcaactcgt ggtgccttta
     2221 tatccataag gataatattg tcagccaatt tttcttttcg aactattgga tacatagtct
     2281 tcctcctagt taaatcttat cccattacat atgtaatctg atactcattt atttcatact
     2341 tttttaattt taaatgaaat gatataattt ttcaatatgt ttctttggat attccataaa
     2401 aatatgttat atgcttatga aattgtgtta tcttttgaca ttcgtccggt actttatatc
     2461 aaaaacagga accaattcat gcaaaaaatg aaccggttcc tgtatttatc agcttttatg
     2521 agaaatatac aagctcatcc tgcggcttct cggcaggctc gatggaaacc ggataaagca
     2581 ccggtccctt tcccttgtac tctcttgcaa actccacatg cacccttgcg gtgatggtaa
     2641 tccacgattt gtgaggaata ctactctcat cctcatactt gcacttaaag cctataaagg
     2701 tcacgtcctc tatacagcag gtcatcgcaa acctgcccgg taccataaca ccctttctaa
     2761 gcttatcagg attgtataca agagcaagaa atttgacaac cttgccgtcg tacttcttgt
     2821 aattctccat ggcatccata taccagatgg cataatctgc atctgaaagc tcaatcacat
     2881 cctgatttat atcaaacggc agttcctccg gacgctcatc aatagtaccg tccttgcgtt
     2941 cgtagactat ctgggctttg cggttgatat tctttatggt gcgccttagg tgtcctctgt
     3001 ctgtatcatc gtccgtcctg ttaaatatga caacatcact cgcaaatacc tgttcctgca
     3061 tcatcgcgcg catattgttc atataaaggt caaatgtagt ggaatcaact gtggcaagcg
     3121 actgcacgat aacccagccc tttggcagct cagcctctat tattttatca atgccccatg
     3181 tgccgttgta ctcgataaac acctgttcag gcaaatactg catgttgagc tcattcagct
     3241 ttgcagctgt gaactcatcc tccgagtcga tctctgcaac ctggatattg gcagccttta
     3301 gcttatccat atcatactcc acatcaccat cctcgcacat aatgataagc ccctttgagc
     3361 cattagcaaa atcattctcg aaaatggtat ccttaatcag tgaagtcttg ccactgtcca
     3421 tgaatccggt aaataaatat actggaatct cttcagccat tttgttagtt cctttctgat
     3481 tattgtgtaa ctatattaaa tacatttaag caagtccgaa aagctcttca agctcatgct
     3541 cgtggatatc tgttccgata acaactatac gtcctgtata gtcaggctcg ccatcacgaa
     3601 tctcgtactc gcccggaacc atatcaaagt aaatccactt gccgtctgtg ccctctacca
     3661 tgccctttga acgaagaatc ttgtcatctt ccacaaactg cttaaggatt tcttcgagct
     3721 tagcgcgctc aaacttgtga ggtgtctcct tgccccagct tgtaaataca tcatcagcat
     3781 gatgatgtcc gtgctcatga tcatggtcat gacagccgca agtgcagccc tcgccatgct
     3841 cgtgatggtg ctcatgttcg tcgtggtcat ggtgatgttc atgctcatca tggtcgtggt
     3901 gatgttcatg ctcatcatgg tcatgatgat gctcgtgctc gtcatggtcg tggtgatgct
     3961 catgctcatc atggtcatgg tgatgatgat gctctgcctc atcctgtgca tgacgcgcct
     4021 cagcaagcac cttcatctcg agattatcct gaccttccat agtcttaaga atctgctctc
     4081 ctttaatagc atcccaatca gtagtgataa ctgaagcctt tggatttaaa gcctgaatct
     4141 gctttacaca aaactcaagc tgctcaggtg tagcattctg gctgcggctt aagatcactg
     4201 ttgtggcata ctctatctga ttgttgaaaa actcaccgaa agccttcatc tgcttgcttg
     4261 ccttaagtgc atttaccact gtaacaagtg cattaagctt gaccggctgt tctttttcga
     4321 tatctataac agatttcatg acatctgata atttacccac gccggaaggc tcgattaaga
     4381 tacggtcagg atgatactta gtaataacct catttaagtt cttaccaaaa tcaccaacca
     4441 gtgaacagca gatacagcct gagttcatct cagtaatctg aatgccggca tcctttaaaa
     4501 agccaccgtc tataccaacc tcaccgaact cattctctat gagaactatc tgctcaccct
     4561 taaaagcctc atctatcatt ttcttgataa atgtagtctt tccagctcca aggaaacctg
     4621 aaataatgtc aatttttgtc attgtaattc actccttttc ttttcactaa gtcctatttt
     4681 actagcaata atacaaatat gcaaattaaa tttttattat taaattatgt tgacacataa
     4741 caaaagaact accgcacgcg gtagtcctct ctaatacatt aattgtattt tctttttcta
     4801 gctgcttcag atttcttctt acgctttacg cttggtttct cgtaatgctc acgcttacgg
     4861 atctcctgct ggatgcctgc ttttgcacag tttctcttga atctgcgtaa tgcgctatct
     4921 aaagtctcgt tttctttaac tattacacta gacataacct aaaacctaac ctccctccag
     4981 ttgtgtattg tgcatcaact gttaggtatc tatacttaat tgcactaaca tgtattttat
     5041 caaaatatcc ggatttgtca agataaatcc ggatattttt tnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncactccaa
     5161 aatgactcta atcaagcttg ataaagattg atgtttcctt tttcagtgaa tctgcaatct
     5221 catagttttc atccatcttg tgtgagatat tgtccatatc ctcaacaata agctgtgtac
     5281 tgtcagccgc tgaaacaact ccgttcacac cctcatttat agcatgtgaa atggtgttga
     5341 ttgaagaaga aatctcaagc atggactgct taagtgaatc agtcttctcc ttgaaatcag
     5401 tcatggtacc ctcaatgaat gaagccttgt cattgtactc cacaccggac tcaacaaatc
     5461 tctcaaactc cggaagaatc gaatcattaa tatagctgac aagaccattg gcgttgtcag
     5521 aaagattgtt aaccgaattg gtaactaccg cattgatacg ctggatattg ttggctgcct
     5581 cctggctggc tgcagcaagc tggctgatct cactggctac aaccgcaaaa cctctgcctg
     5641 cctctcctgc ccttgctgcc tcaatagaag catttaatgc cagcagattg gtctgacttg
     5701 caatgtttaa aatatcattg gtaagtgaat tgacctgctt tacgctgttg ctatcctcaa
     5761 tagccttctg caatacctcg agaatctccg aaaccttcct gtctgttgac tccatgtttg
     5821 tacgtgcaac agactccatt gactgtgcat gggccttcat atccctggtg tatccggtca
     5881 gttcatctgt cttttcagca ataagctcaa cctcttttgc cacattatct gtgttggtat
     5941 tgataacaga tgcattttca gagatatcct gcatggtagc agacagctcc tctgttaaag
     6001 ccgaaagatc ggacacactg ctgttgcttg tctgaacact ctgacgcaca tcatcaacaa
     6061 ccctctccat cctggccgag ttggtgacaa cagccttgaa tatagcctgc agctttgtca
     6121 taaagacatt gatgcccttg cctacggcgt caacctcagc attgttgata ggagtgaccc
     6181 tctgtgtcaa atcgcccttc ttgttatcaa tattgactat gatgccgttg atttccttct
     6241 gtgtacgaag cagaggcttg ataaccatgg tgacaacagc aaaaacaaca aacagtaatg
     6301 caattatact tactgcaata gtaaatgtac atgtgacaac agatgcgcga taagtacttt
     6361 cgaggctctt tctctcctcc tgtgtaacag aattgacatg ctcacgcatt gacgaaatag
     6421 ctttttcaat gttattagta tattttgcaa tagagccatt tgccgtttta tacgcttcct
     6481 cgtttttacc ggcggcagaa taggccatta cattgccaca ctccttgacc aggcttgtat
     6541 agttttcaca gataattcta tagtttctct tggtctccaa ggttacataa ggattataat
     6601 catcaaggag ctgctttagc ttttcctcgt gatttctcac ttcacttaca atatcaatca
     6661 ttgatgatag atctgtcgat ataatatgag acagtgccag tgtatgaata ttcacggtct
     6721 ccatctcaat atcattaagc atctccgtgc caaccattga ggtgtccaca atctgagaag
     6781 ccgcatcatt tacctttgat aaatttttaa gagataaacc acttgaaata acacttacaa
     6841 agcctacaat aaaaacagga ataagtatca attttgtact taactttttc tttcccttga
     6901 cctttggttt gttattaacc ttttgagctt tttttgtgtt gagagttttt ttaacttttg
     6961 gtgcttttgc aggatttgct gcttttgctt tcaccttttt ttgttttact gcgctatttt
     7021 taacagctgc tttacttttt tttgaatcta atttcatgtt atatacccct ctactcagct
     7081 acagattgtg ttatccccgc aactagatta tccattatct cctgaagatc tgtattatca
     7141 ggattaccgg ccgccatttt ttcgccaaac tccgtcgcag catcccagta agaatttcca
     7201 actctttgga gctttccgta ctgtgcctga tccataacag ccataatagc aggaaccttt
     7261 ttgaccgcat catctgcggc tgccgcttta ttggcagggc ctacattctt catctcaaag
     7321 cgtagcttct ggttatcctc gtttgtaaac cactgggcaa gcttatttgc ccactcagga
     7381 tacttggtgt atgcatttac agccatatat ttatagcctt taaacgatga catctgaatc
     7441 tgctgtccgg cacatgtgta tgtaggcagc tttacagcac cataatcatc gccccatatt
     7501 tccttgattt ttgctgcatt ccagcatcca ctgacaccgg caataatgct tccgtcctgt
     7561 actcctgcaa taaaatcgtc ctgtacacag ttcttaaagc ccggcttagc cgcaatatca
     7621 agcatagcct gtgctatatc aacacccttg atgtccgtga tgatagaatt ccagttgcag
     7681 tggtttgtca caccatcatc attcacgcca aaatcaagcc ctgtatttcc aaaaaatgaa
     7741 tacagatacc agcctgagct ccagtccata gtcaagtact taccattggc ccctgcaatg
     7801 tcaagcatgc catcaagcgt agccacatcg ctgtctgaga aataattctt attgtaatac
     7861 ataaaatatc cattgtcagc ggtcagcgga tatccatata atttgccatc gatagtggca
     7921 gcctcaaccg caccttcatc gtcagccttt ttaacggcat ccacatcctc tatctcataa
     7981 agagcgcctc ccgctaccat agaagttatc tggtcatccg ctatag
//
LOCUS       DPUF01000068            7963 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3182, whole
            genome shotgun sequence.
ACCESSION   DPUF01000068 DPUF01000000
VERSION     DPUF01000068.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3182"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            36..296
                     /locus_tag="DHV81_02390"
     CDS             36..296
                     /locus_tag="DHV81_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93372.1"
                     /translation="MKMQKVTDSFELPKDIVLGLPIVTAYGYNELTIENHKGILSFSD
                     KAVTVRAADSTIKISGSRLEIKEFTKDLIIIAGHLKGVIYEY"
     gene            286..813
                     /locus_tag="DHV81_02395"
     CDS             286..813
                     /locus_tag="DHV81_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HCI93373.1"
                     /translation="MSIKAYATVRLTGCNIRRFINLCTANHIKIWNLKYVSPKEYEAC
                     CSTEDIFLMKPHLKKTHTRLLVVKRQGYFAIRAFLYKIRIITAAITGVFCIHALICTR
                     IWHIVPEGNRFTYDESVISFMESENVTIGSHKRADYSLLEKKLEEKYPDITWCSIYIE
                     KNTMKIDISEGINYR"
     gene            878..1890
                     /locus_tag="DHV81_02400"
                     /pseudo
     CDS             878..1890
                     /locus_tag="DHV81_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516392.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
     assembly_gap    1534..1602
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1887..3047
                     /locus_tag="DHV81_02405"
     CDS             1887..3047
                     /locus_tag="DHV81_02405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93374.1"
                     /translation="MRRVVSLISIFISILALSFVLCLLGDVYPDEWICMGFLDIIFYM
                     LLLFELEYERNTLQLSNNSRTDYLRFTFAFIICSIVCIISGFMPLYSRPVMIFPILLC
                     LIGNEFLAFISGTYFCILLSITVSGDCFELVCELLLVITGAILAKMLKEDKLQICIYL
                     IIISMSIVTPGIFYYMSTKEFSVSVIIAGAVSGMIVSLIGIICARVFKPLSADETNDR
                     LIAIIEEDFPAVKQLKKNNFSEYNHGNFVSTIAIKAAKAAGLDTALCAAGGFYYRIGQ
                     WQKHKSVMEGVEQALAMHFPEKLTNILYEYYGKLRHPQTPESALIHMVDALIVRLDHI
                     KNDVADSEWNHEILIIQTLNELSSSGMYDESGLSMNHFLKIRDYLTKEELLK"
     gene            3044..3541
                     /locus_tag="DHV81_02410"
     CDS             3044..3541
                     /locus_tag="DHV81_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCI93375.1"
                     /translation="MSFFIEKEVECNFNFDYEKVAEAVVSASLEHESFPYEAEVNLTL
                     TDNEGIHAINKEYRQIDRPTDVLSFPMLSYETPGDFSFLSDENEDDFNPDTGEVMLGD
                     IIISVDKVKEQALEYGHSEKREFAFLITHSMLHLFGYDHMEADEAAVMEEHQRKILDA
                     LGITR"
     gene            3581..5245
                     /locus_tag="DHV81_02415"
     CDS             3581..5245
                     /locus_tag="DHV81_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCI93376.1"
                     /translation="MAKKNNGASNALVQGSILAVASIISRIIGLIYRIPMQRILGDIG
                     IGYYSTAFEIYNVMLIISSYSLPLAVSKMVSAQMAKKRRTATYQILKCALLFATISGT
                     TVALILFFGGNFFATTLLNTPYSIFALRVLAPGLLVVAVLGVIRGFFQGLGTMMPSAV
                     SQIIEQIVNAIVSVWAAYVLYRTGTRIGAVLGDKEHYAAAYGAAGGTLGTVLGSVAAL
                     LFVVFIFAIYMKVFKRQMKRERNVRVSSFGYTMKILVITVIPVLLSTTIYNISGIIDQ
                     GIFKQVALLQGYTQNDIDVWWGVYTGKYKLLINVPISIASAMAASFVPVLTGAYHRDD
                     MEAVRGQINLSTRFIMVVAFPCAVGLAVFGLPIFNILFSSTRATNAEASLMMYVGAVA
                     VVFYSLSTLSNGLLQGIDRLKVPVINAAISIVAHVIVLILLMLIFRLNIHAVVLANTF
                     FALLMCFMNSMALKKYSGFKQEIKKTFIIPAISSLIMGVISYILYFILYKACHIEIIA
                     FILAAIIAVISYAVALLLLKGLTEDELRHFPKGTLIIKVAKKCHLL"
     gene            5295..5669
                     /locus_tag="DHV81_02420"
     CDS             5295..5669
                     /locus_tag="DHV81_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93377.1"
                     /translation="MKSKISICFLTIVVILLALVMIEKINTQKAADKAAITAQRNEND
                     KEEKNEKESLLTSNEAKNAKYCIKNDSGMLSVYNATASEKYFDTGVYFEELPDEAKNK
                     VTNGLYFFNETDLYDFLESYSS"
     gene            5700..6974
                     /locus_tag="DHV81_02425"
     CDS             5700..6974
                     /locus_tag="DHV81_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HCI93378.1"
                     /translation="MYDVIIIGAGASGLMAAAAAASKGARVALLEHKDDIGKKILATG
                     NGRCNFTNTDMSVNKFHGSKALIKNGLSQFNYSDTIRFFKELGIPAYDNAGYIYPNSR
                     QATSVVAAFRMELMRLHVDVKTGISITEIKTAGITAEDTNSAANPATNKKTDDRTGYC
                     IQTDKGSFKSKRLIIACGLTASPKLGSDGSLFRQIEALGHHIQKPLPALCGFSCDGLN
                     FKKITGVRCDATVASVIDGQMTEQNTGELQLADYGISGIPVFQISSLMSRALDKGQRV
                     EVIIDFLPAFSDDELNGYIKDRSITTTDNRSLNEMLNGLLNNKLLLELIHKSGVSPDK
                     KGRLLTDDDCKSLTRSIKHTAVSVKKPRGAEFAQVCAGGIYTKEIDVRTLESKIHPGL
                     YFCGELLDVDGICGGYNLQWAWTSGYIAGEMQ"
     gene            6975..>7963
                     /locus_tag="DHV81_02430"
     CDS             6975..>7963
                     /locus_tag="DHV81_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCI93379.1"
                     /translation="MIKINQIKLPVTHSDEALKKKIIKMLKLNAKTGFTYRILKKSLD
                     ARKKPELFYTYSVAVELDDTKNSEESIVKRAASSSVLIYKEKEYMIPACGHIPLDRRP
                     VIAGAGPAGLFCAYILAEAGFRPIVIERGSRVEKRTCDVQKFWESGILNPKSNVQFGE
                     GGAGTFSDGKLNTSVKDPSGRNRLVLETFVRFGADPSILYDNKPHIGTDVLSEVIVNM
                     REFLVDKGATFVFDTCVNNLDIVSQKLLSVYTDSDSNSNSEIKTDVCVLALGHSARDT
                     FDMLYNKGFDMECKSFAVGFRVEHPQHMIDESQYGIQNKIILPPSPYKVTSNFP"
BASE COUNT         2459 a         1466 c         1802 g         2167 t
ORIGIN      
        1 ggcataattc agcctaaata aacatatgtt aatatatgaa gatgcaaaaa gtaactgatt
       61 cgttcgagct tccaaaggac atagtgctag ggcttccaat tgtaacagca tatggttata
      121 atgagttgac tatagaaaac cataagggta ttctgtcatt ttcggacaag gccgtaactg
      181 taagggcagc agacagtacc ataaaaatat ccggcagccg ccttgaaatc aaggaattta
      241 caaaggattt aataataata gccggtcatt taaagggtgt tatatatgag tattaaagct
      301 tatgcaaccg taaggctaac aggctgcaat atcaggcgct tcataaatct gtgcacggca
      361 aaccatataa aaatatggaa ccttaaatat gtaagtccaa aggaatatga ggcctgctgc
      421 agtacagaag atatttttct aatgaagccg cacctgaaaa agacgcatac aaggcttctc
      481 gtggtaaaaa gacagggata ctttgcaata agagcatttc tatataaaat acgtattatt
      541 acggctgcaa ttaccggcgt tttttgtata catgcactca tctgcacaag aatctggcac
      601 attgtgcctg aaggaaaccg tttcacgtat gatgagtcgg tcatctcctt tatggagagt
      661 gaaaatgtaa caataggcag ccacaagaga gcagattaca gcctccttga aaagaagctt
      721 gaggaaaaat atcccgatat cacgtggtgc agtatataca tagaaaaaaa taccatgaaa
      781 attgatatat cagagggaat aaattatagg taaattatga caaattttat ttttatgtta
      841 taatgaaaaa tattttgatt gaaaggcaca tgagctaatg agtttaaatg aagtatctat
      901 aaaaataccg ggtgaatata ttccaaatgt ctttggacag tttgatgcat tcgccaagaa
      961 aatagagcgc acactgcacg taacgcttgt cttaagagat gattccttaa agattatcgg
     1021 agcgcaggga aatattgatg gggcaaaaga ggtgttcgag cagctcattg cgctggctga
     1081 gagaggcaac gtcattacag agcagaacgt aaactatgca cttgcacttt tagaggagca
     1141 tagaggcagt gagattgttg agattgacaa ggatatcatt tgtcacacaa taaatggcaa
     1201 gcctgtaaag ccaaaaacca taggccagaa ggaatatgtc gatgcaatca ggaaaaagat
     1261 gattgtgttt ggcatgggtc ctgccggaac aggaaagacc tatcttgcca tggcaatggc
     1321 tataacagct tttaaaaatg agcaggtggg ccgtattatt ctgacacgtc ctgcgattga
     1381 agcaggcgag aagcttggct ttctgccggg agatctgcag agcaagattg acccatattt
     1441 gagaccgttg tacgatgctc tttatcagat aatgggagct gagagctttc agcgcaacat
     1501 ggaaaagggc cttattgagg tggctcctct tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagaatac aactcctgca
     1621 cagatgaaga tgtttcttac acgaatcggc tttggctcaa aggttgtcgt gacaggtgat
     1681 gcgacacaaa aggatcttgc accgggagca aaatcagggc ttgatgtggc acttagggtg
     1741 cttaaaaaaa tagatgatat aggcatatgc aatctgacaa gcaaggatgt cgtgcgtcat
     1801 ccgcttgtac aaaaaattgt tcaggcttat gatgattatg aaaataagca gactgcaaag
     1861 actgtaagaa agcagaggaa gagcaattga gaagggttgt atcactaata tcaattttta
     1921 tatcaatctt agctctttca tttgtgctgt gtctgcttgg tgatgtatat ccggatgagt
     1981 ggatatgcat gggtttttta gatatcatat tttatatgct gcttttattt gagcttgagt
     2041 acgagcggaa cacactgcag ctgtcgaaca actcaagaac cgattatctt aggtttacgt
     2101 ttgcgtttat catttgctct atagtgtgta tcataagcgg ttttatgcca ctgtacagca
     2161 ggccggtgat gatttttcct atattgcttt gtctgattgg caatgagttt ctggcattta
     2221 taagcggtac ttatttttgt atattgcttt caatcactgt atccggcgac tgctttgagc
     2281 tcgtatgtga gctgcttctt gtaatcacag gtgctatcct tgcaaagatg ctaaaggagg
     2341 acaagcttca gatatgtata tatctgatca taatcagtat gagcattgtg acaccgggca
     2401 tattctatta tatgtcaaca aaggagtttt ctgtttccgt tattattgcc ggagctgtaa
     2461 gcggcatgat tgtttcgctt atcggcatca tttgtgccag ggtatttaag ccacttagcg
     2521 ctgatgaaac aaatgacagg cttatagcaa taatagagga ggattttcct gctgtaaagc
     2581 agcttaaaaa gaacaatttc tctgaataca atcatggcaa tttcgtatcg acgattgcca
     2641 taaaggctgc aaaggctgca ggactggaca ctgcgctatg cgcagcagga ggcttttatt
     2701 accgcatagg gcagtggcag aagcataaaa gcgtcatgga aggagtagaa caggcattag
     2761 ccatgcattt tcctgaaaag ctgaccaata tactctatga gtactatggc aaactaagac
     2821 acccacagac ccctgaatcc gcactcatcc atatggtcga tgctttaata gtaaggcttg
     2881 atcatatcaa aaacgatgtg gcagacagtg aatggaatca cgaaatactt attatccaga
     2941 cattaaatga gctgtcaagc tcaggaatgt atgatgaatc agggctttcc atgaatcact
     3001 ttttaaaaat acgagactat cttacaaagg aggagctgct taaatgagtt tttttatcga
     3061 aaaagaagta gaatgcaatt ttaattttga ttatgaaaag gtcgcagaag ctgttgtttc
     3121 tgcttcactt gagcacgaga gctttccgta tgaggcggag gttaatttga cactgacaga
     3181 caatgaaggg atacatgcca tcaataagga gtacagacag atagacaggc ctacagatgt
     3241 actgtctttt ccgatgcttt catatgaaac acccggagat ttttctttcc tttcagatga
     3301 gaatgaggat gatttcaatc ctgatacagg agaggtcatg ctaggcgaca ttatcatatc
     3361 cgtcgacaaa gtaaaggagc aggccttaga atacggacac agcgaaaaaa gagagtttgc
     3421 ctttctgatt acacacagca tgcttcatct gttcggatat gaccacatgg aggctgatga
     3481 ggctgccgta atggaagagc accagagaaa aatacttgac gctttaggta taacacgcta
     3541 atatttaagc tggttatcaa ttgaaacaga ggatttataa atggctaaaa aaaataacgg
     3601 ggcaagcaat gccttagtac agggttctat acttgccgta gcatctatca taagcagaat
     3661 aataggactc atatatcgca taccgatgca gcgtatattg ggcgatatcg gaatcggcta
     3721 ttattccaca gcctttgaaa tatataatgt catgctgatt atatcatcat acagcttacc
     3781 acttgccgta tcaaagatgg tttcagccca gatggcaaaa aagaggcgga ctgcgacgta
     3841 tcagatactg aagtgtgctc tgctttttgc aaccatcagt ggtacaacgg tggcactcat
     3901 actgtttttt ggtggcaatt tttttgcaac aacgcttctt aacacaccat acagtatttt
     3961 tgctttaaga gtactggctc cggggctttt ggtagttgca gtgctcggcg tcatcagagg
     4021 ctttttccag ggactgggta ctatgatgcc gagtgccgtc tcacagataa tagagcagat
     4081 agtaaatgcc atagtcagtg tatgggctgc atatgtgcta taccgtacag gtacaaggat
     4141 tggcgcagtg cttggtgaca aggagcacta tgccgctgca tatggtgcag ccggtggtac
     4201 acttggaaca gtgcttggct ctgtagcggc tcttttattt gtagtattta tctttgccat
     4261 ctatatgaag gtgttcaaga ggcagatgaa aagagaacga aatgtcaggg tgagctcatt
     4321 tggctacaca atgaagattc ttgtaattac ggttattcct gtgcttttaa gtactactat
     4381 ctacaatatc agtggtatta tcgaccaggg tatattcaag caggttgcac tgcttcaggg
     4441 atacactcag aatgatatag acgtatggtg gggtgtatat accggaaagt acaagctttt
     4501 aatcaatgtg cctatatcca tagcatcagc tatggcagca tcctttgtgc cggtgcttac
     4561 aggcgcatat cacagggacg atatggaagc agtgagagga cagattaatc tttccacacg
     4621 ctttatcatg gttgtcgcat tcccatgtgc agtagggctt gccgtgtttg gacttcctat
     4681 atttaatata ctgttttcaa gcacacgtgc aacaaatgct gaggcatcac ttatgatgta
     4741 tgtcggtgcg gttgcggtag tattttactc tctttccact ctgtccaacg gactgctgca
     4801 gggcattgac aggctcaaag ttccggtaat caacgctgcg atatcgattg tagcgcatgt
     4861 catagtactc atactgctta tgctcatatt caggctcaat atacatgcag ttgtgcttgc
     4921 caatacgttt tttgcattgc tcatgtgctt tatgaacagc atggctttaa agaagtacag
     4981 cggctttaag caggaaataa aaaagacctt tatcataccg gccatcagct cgctcataat
     5041 gggagttatt tcgtatatat tatactttat actttacaag gcctgtcata ttgagattat
     5101 cgccttcatt cttgctgcaa tcattgcggt tatctcatat gcagtggcgc tgcttttact
     5161 caagggactg actgaggatg agcttagaca ttttccaaag ggcacactta tcataaaagt
     5221 ggcaaaaaaa tgtcatttat tataaaattg aataaataac aaaaatttac agatactgat
     5281 tttgaggtga taatatgaaa agcaaaatca gtatctgttt tttaactatt gttgtaattc
     5341 ttcttgcact tgttatgata gaaaaaatca atacgcaaaa agcagcagac aaggctgcga
     5401 taactgcaca aagaaatgaa aatgataaag aagaaaaaaa tgaaaaggaa agccttctga
     5461 catcaaatga ggctaagaat gcaaaatact gcatcaaaaa tgactccgga atgctgtctg
     5521 tatacaacgc cactgcaagt gaaaaatact ttgacacagg cgtgtatttt gaagaactgc
     5581 ccgatgaagc aaaaaataag gttacaaacg gactctactt ttttaacgaa actgatttgt
     5641 acgattttct tgaaagctat tcaagttaat gttaaaataa atcagttagg ggaaagaata
     5701 tgtacgatgt aattataata ggtgccggtg catccgggct gatggcggca gcagctgctg
     5761 catcaaaggg agcacgtgtg gcacttttag agcacaagga tgatatagga aagaaaatac
     5821 ttgcaacagg aaacggcaga tgtaatttca caaacactga tatgtccgta aacaaattcc
     5881 acggcagtaa agcactgatt aaaaacgggt tgtcgcaatt taattattca gacacaatac
     5941 gtttttttaa agaacttgga atacctgcat atgacaatgc aggctacatt tatccaaatt
     6001 caagacaggc tacatctgtt gtggcagcct ttcgcatgga gcttatgagg cttcatgtgg
     6061 atgtgaaaac aggcattagt attacagaaa tcaagactgc agggattacg gctgaggaca
     6121 caaattctgc tgctaatccg gcaactaata agaagacaga tgaccggacc ggttactgca
     6181 tccaaacaga caagggcagc tttaagtcca aaaggcttat tatagcgtgt ggccttacgg
     6241 cttccccaaa gcttggaagt gacggaagcc tgtttcgaca gatagaagct ctcggacacc
     6301 atatccaaaa gccattacca gcgctttgcg gctttagctg tgatggccta aatttcaaaa
     6361 aaatcacagg tgtcaggtgc gatgccactg tcgcttccgt gatagacggt caaatgacag
     6421 agcaaaatac aggggagctg cagttagctg actatggcat ctcaggcatc ccggtttttc
     6481 agatttcaag tcttatgtca agagccctcg ataaaggtca aagggtcgag gtgataattg
     6541 actttttgcc ggcttttagt gatgatgagc ttaatggata cataaaggac agaagcatta
     6601 caacgacaga caaccgctcg ttaaatgaga tgcttaacgg acttttaaac aataagcttc
     6661 ttcttgagct gatacacaaa tcaggtgtat ctcctgacaa aaagggaagg ctgttgacag
     6721 atgatgattg taaaagtctc acccgctcaa taaagcatac agccgtttcg gtaaaaaagc
     6781 caagaggagc cgagtttgca caggtgtgtg caggcggcat atatacaaag gaaatagatg
     6841 tacgtacatt ggagtccaaa atccatccgg ggctttactt ttgcggcgag ctgcttgatg
     6901 tggacggcat atgcggtggc tacaatctgc agtgggcatg gacaagtgga tatatagcag
     6961 gagaaatgca gtaaatgatt aaaattaacc agataaagct ccccgtcacg cacagtgatg
     7021 aggcattaaa aaagaaaata ataaaaatgc ttaagctcaa tgccaagaca ggctttacat
     7081 accgcatatt aaagaaatca cttgacgcgc gtaaaaagcc tgagctgttc tatacatatt
     7141 cagttgctgt agagctagat gatacaaaaa atagcgaaga atcaattgtc aaaagagcag
     7201 ccagctcatc tgtgctcata tacaaagaaa aagagtacat gatacctgca tgcggtcata
     7261 taccgcttga ccggcgacct gttattgcag gggcaggtcc tgcagggctg ttttgtgcat
     7321 atatattggc agaggcaggt tttcgcccga tagttatcga gcgtggcagc cgtgttgaga
     7381 aaagaacctg cgatgtacaa aaattctggg agagcggcat acttaatccc aaaagcaatg
     7441 tacagtttgg tgagggcggt gccggaacct tttcagatgg caagctgaat acatccgtaa
     7501 aggatccttc gggcagaaat cgtcttgtgc ttgagacctt tgtgcgcttt ggggcagatc
     7561 cgtccatact ttatgacaac aagccacata ttggtactga tgtgctttca gaagtgattg
     7621 tcaacatgcg tgagtttttg gttgacaaag gggctacatt tgtatttgac acctgtgtaa
     7681 acaatcttga catagtttcc caaaagctat taagtgttta cacggattct gacagtaaca
     7741 gtaatagtga gataaagaca gatgtatgcg ttctggcact cggacacagc gccagagata
     7801 catttgatat gctttacaac aaaggctttg atatggaatg caagagcttt gcagtcggct
     7861 ttcgcgtgga gcatccgcag cacatgatag atgaatcaca gtacggtatt cagaataaaa
     7921 tcatacttcc tccaagtcct tacaaggtca catccaattt tcc
//
LOCUS       DPUF01000069            9316 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23381, whole
            genome shotgun sequence.
ACCESSION   DPUF01000069 DPUF01000000
VERSION     DPUF01000069.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23381"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="DHV81_02435"
     CDS             complement(<1..770)
                     /locus_tag="DHV81_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCI93380.1"
                     /translation="MRDRFGREIDYMRISVIDSCNLNCYYCNPQDNNKKCQAINILSV
                     KKVLCIVRAATRLGITHFRLTGGEPLLHPQIDEMVSQIKKIPGVRSVSLTTNAVLLAQ
                     HAKQLKEAGIDSINVSLDTIDASEYEHITKKPQLDKVEQGIDAAIECGIRMKINVVLT
                     PQTDVVALTRYASKKGTDIRFIEMMPVGEGHTNGVEPYKKVIGTLSELYGEPCHVNTG
                     KTKEINSGYNKYKEERKNPDNGPAEYYIFPELDIRVGL"
     gene            complement(772..1635)
                     /locus_tag="DHV81_02440"
                     /pseudo
     CDS             complement(772..1635)
                     /locus_tag="DHV81_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743920.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
     assembly_gap    1405..1481
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1636..1754
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1755..1964)
                     /locus_tag="DHV81_02445"
     CDS             complement(<1755..1964)
                     /locus_tag="DHV81_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HCI93381.1"
                     /translation="MKLMKTTEAVGQVLCHDITQIIPGVKKDAVFRKGHIITKEDIPV
                     LLSVGKDTIYIWENDETMMHENEAAE"
     gene            complement(2034..2522)
                     /locus_tag="DHV81_02450"
     CDS             complement(2034..2522)
                     /locus_tag="DHV81_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HCI93382.1"
                     /translation="MYTVGIITISDKGAAGQREDKSGPKIKELLPKDKYDVVSYKIIP
                     DERKQIADELVRLCDDVKCNLVLTTGGTGFSPRDITPEATMDVAERNAPGIAEALRAY
                     SMSLTPKAMLGRGASVIRKNTLIVNLPGSVKAVSESMDFLMGNIEHGLDILLQYDSEC
                     GR"
     gene            complement(2608..3150)
                     /gene="moaC"
                     /locus_tag="DHV81_02455"
     CDS             complement(2608..3150)
                     /gene="moaC"
                     /locus_tag="DHV81_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCI93383.1"
                     /translation="MRINRLTQENELDINNQKQNKGKLTHFNEAGEAHMVDVHAKDDT
                     YRVAKACGTIKVNGAVYRAVSTGTAKKGDVLAVARIAGIMGAKNNSMLIPLCHAIAMT
                     KCDVEFELDEKNSSITAICTTACVGKTGVEMEAMTGVSVALLTIYDMCKALDRGMEIG
                     QIHLLEKSGGKSGHYVTDHN"
     gene            complement(3203..4165)
                     /locus_tag="DHV81_02460"
     CDS             complement(3203..4165)
                     /locus_tag="DHV81_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCI93384.1"
                     /translation="MSMNQSNNQIIKKNLLIICRGAGDLATGIIHRLHRAGHRVIALE
                     TDYPAAIRRQVSFCEAVYDGSAAVEGVTARLVPALADAETDTETYSGINDTPAAHIAS
                     EKWDSSAIEAVLEAGEVPLLIDPKGESIALLKPDVVVDAIIAKKNLGTTINMAPLVIG
                     VGPGFTAGQDVHLVIESMRGHNLARIITDGMARPNTGVPGNIAGFTSERVIHAPAAGY
                     IHDVRKIGDIVQKGDEIARIYPDKESYDNALSEYVPVNATITGIIRGLIREGYYFREG
                     FKIADIDPRESELTNCFTISDKARSIAGSVLEAVSAFEHGVKIY"
     gene            complement(4212..4829)
                     /gene="yqeC"
                     /locus_tag="DHV81_02465"
     CDS             complement(4212..4829)
                     /gene="yqeC"
                     /locus_tag="DHV81_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCI93385.1"
                     /translation="MEKVISIVGAGGKTTLVHKLAREYHRSGKGVLVTTTTHMYVEAD
                     TDLSCDFFALRDKIIKDGYCMAGQKISEQKISEQSKSKMCGLPYDLLDKLIKDMPQAL
                     DYVIIEADGAKHHSLKYPAADEPVIYPGTTDVIIVLGTWEKGRSCKDVVFRYELMQEE
                     LGTAADAVVDDSIIDTLRQVYVRKLRDSGFEGRVSCVFANERGWF"
     gene            complement(4906..6408)
                     /locus_tag="DHV81_02470"
                     /pseudo
     CDS             complement(4906..6408)
                     /locus_tag="DHV81_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515888.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
     assembly_gap    5959..6046
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6628..>6888)
                     /locus_tag="DHV81_02475"
     CDS             complement(6628..>6888)
                     /locus_tag="DHV81_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HCI93386.1"
                     /translation="ECPVYLLFEEPEKYVAKDGAESFEELYHRTGEFIEQVLRPQLEA
                     GKNVLVVGHGAMNCSIVNQFRGTPIKDFWKDMQGNCELLRVE"
     assembly_gap    6889..7039
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7040..7191)
                     /locus_tag="DHV81_02480"
     CDS             complement(<7040..7191)
                     /locus_tag="DHV81_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HCI93387.1"
                     /translation="MFYILRHGRTDWNEEHRLQGEVDIPLNETGRQMAYDAAEKYKDI
                     DFDICY"
     gene            complement(7194..8621)
                     /locus_tag="DHV81_02485"
     CDS             complement(7194..8621)
                     /locus_tag="DHV81_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCI93388.1"
                     /translation="MWNTINIFLKAVDDFVWGVPLMVLIMAGGIMLTVRLGLLQMRKL
                     HLALKWMVKNEEGGDGEITSFGALCTALSATIGTGNIVGVATAVGAGGPGALFWMVLA
                     AFFGMATKYSEGLLAVKYRVIGKDGHSLGGPFYYIEQGMGAKWKWLAKIFAFFGVCVG
                     LFGIGTFSQVNGISSAVNNFFDPKNQHTVKVLPFLGEYSWSVVIASLVLAFCVAAVLI
                     GGVKRIASVSQIIVPFMAVIYIVFVLVLVVCNITAVPAAFATIVKAAFSPKAITGGAV
                     GSFLVAMQKGVARGIFSNEAGLGSAPIAAAAAQTKEPVRQGLVSMTGTFIDTIVICTL
                     TGLSIVLTGAWQVDGLEGVQVTTYAFQNGLPLPKELSAFVLMLCLVFFAFTTILGWDY
                     YSERCLEYLSGGRMKYVKVYRWIYILAVFIGPYMTVSAVWTIADIFNGLMALPNMIAL
                     FALSGVVVKETRHFFERHRNGEIED"
     regulatory      complement(8827..8973)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(9161..>9316)
                     /locus_tag="DHV81_02490"
     CDS             complement(9161..>9316)
                     /locus_tag="DHV81_02490"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743930.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCI93389.1"
                     /translation="EEYNSMEVMFYNAMNEAKSVMKLKKGDHIVLTGGLINGKTGNTN
                     VIKVETV"
BASE COUNT         2294 a         2344 c         1656 g         2587 t
ORIGIN      
        1 atcagcccta ctctgatatc aagctccgga aaaatgtaat actctgccgg gccgttatcc
       61 ggattcttac gttcctcttt atatttatta tatcccgaat taatttcttt tgtttttcca
      121 gtatttacat ggcaaggttc tccatacagt tctgaaagtg tacctataac cttcttatac
      181 ggctcgactc cgttggtatg cccttcacct accggcatca tctctataaa acggatatct
      241 gtgccttttt ttgaagcata ccgtgtgaga gccaccacat ctgtctgcgg tgtgagcacc
      301 acattgattt tcattcttat tccacactct atggcggcat cgattccctg ctccacttta
      361 tcaagctgcg gtttttttgt gatatgttca tattctgacg catctatcgt gtccaggctc
      421 acatttatac tgtctatgcc ggcttcctta agctgctttg catgctgcgc aagcagtact
      481 gcgtttgtgg tgagcgatac actcctgaca ccgggtatct ttttgatttg tgaaaccatc
      541 tcatcaatct gcggatgcag aagcggctca ccgcctgtca gacgaaaatg cgtgattcca
      601 agccttgtgg ctgcgcggac tatgcaaagc acctttttta cgctaagtat atttattgcc
      661 tgacactttt tattattgtc ctgtgggttg caataataac agttcagatt acagctatct
      721 atcaccgata tgcgcatgta gtcaatctct ctgccaaatc tatcgcgcat attaaaagcc
      781 cttcccgaaa ccgcagttag gaaacgtgca gacatcacaa ttcaggcaga gtccgccttc
      841 tccaagtccg gcaagctcat ctgccaaaat catatcacac gccataagcc tcggcaatac
      901 aatatcaaat atcgtgcgct tgttgtacat ggcgcagcca ggcagtccac atatcggcac
      961 cagcctgtca cctgcatcat agtacgacag caaaaacatg gctcccggca gcaccggtga
     1021 accgtacgac acgatccgcg cgcctgtatt ttttatggca agcggtgttt tatcatcagg
     1081 atccacgctc attcctcctg tgcagaatac aatctccgct cctgcctcta ttgcacgtaa
     1141 gcatccgtct gttatttttt ggtcatcatc atcaaacacc tcatgaaaca tcatgtgtgc
     1201 accaaactct gctatttttt tctcgatgac aggggtaaac gcatcctgta tcctgccgtg
     1261 gaacacttca tttccggttg tgattatcgc tgcttttctg accacaaacg gctttatatc
     1321 aagaatcggt ccgtcatcgc atatgtgcga agccgcctct agcttatcct tttttatgac
     1381 aagcggtatg attcttgttc cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgtgcctc gtggctatca
     1501 tcatttcacc aaaggagttg acctttttca gcttttcgct gtccacctta agcagtccgt
     1561 cacagtcagc aatcacctct atcttgcctt cctttacagg ggatggatgc attttacttg
     1621 cggtgccacc aaaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnctctgc tgcctcattt tcgtgcatca tggtctcatc attttcccat
     1801 atatatatgg tgtcttttcc gacgctaagc agcaccggta tatcctcttt tgttataata
     1861 tgccccttgc gaaaaaccgc atcctttttc acaccgggga taatctgtgt gatatcgtgg
     1921 cagagcacct gtccgactgc ctccgttgtc ttcataagct tcatataatc ccacactcca
     1981 aatattattt ttgtaaatag ttactgtcct aaaccatacc ccgtgcattt ctgtcaccgg
     2041 ccgcactcac tgtcatactg cagtaaaata tcaagcccat gctctatatt tcccatcaga
     2101 aaatccatgc tctccgacac tgccttcaca ctgccgggca gattgactat cagtgtgttt
     2161 ttcctgataa ctgaagctcc tctgccaagc atggctttag gtgtaagact catgctgtac
     2221 gctctcagtg cctcagcgat acccggtgca ttacgctctg caacatccat tgtagcttcc
     2281 ggtgtgatat ctctcggtga aaaaccggta cctccggttg taagcaccag attgcatttc
     2341 acatcatcac acagcctgac aagctcgtct gcaatctgtt tcctctcatc cggtattatt
     2401 ttatatgaaa ccacatcata cttatctttg ggaagcagtt cctttatttt cggaccgctt
     2461 ttatcctctc tttggcccgc tgcgccttta tcgcttatcg ttataatccc tacagtatac
     2521 attacatcac cctgttatcc ttgattaatt gtgtattttt aacttctcag cattttaaat
     2581 gagctgtaat cgttgccggt cacattgtta gttgtgatca gtaacgtaat gtccgctctt
     2641 gccaccggat ttttccagca gatgtatctg cccgatttcc atgccacggt ccagtgcctt
     2701 acacatatca tatatggtaa gaagcgccac actgacccct gtcatggcct ccatctctac
     2761 accggttttt cccacacagg ctgtcgtgca gattgccgtg atactgctgt ttttttcatc
     2821 cagctcaaac tcaacatcgc actttgtcat ggcaatagca tgacagagcg gaatgagcat
     2881 ggaattgttc tttgcgccca tgattcccgc aatcctcgct actgccagca catcgccctt
     2941 tttagctgtg cccgtagata ccgccctgta tacggcgcca tttaccttta tcgtgccaca
     3001 ggctttcgcc acgcggtatg tgtcatcctt tgcatgcaca tccaccatgt gagcttcccc
     3061 tgcctcatta aaatgtgtga gctttccttt attttgtttc tgattattga tatctaattc
     3121 gttttcctgc gtcaatctat ttatcctcac tgtaactatt cagaaccact aaaacacctt
     3181 aatagttacc ctcatttatt atttaataaa tctttacgcc gtgctcaaat gccgataccg
     3241 cctcaagcac actgcctgct atacttcttg ccttgtcaga gattgtaaag cagtttgtta
     3301 gctcactctc gcgcgggtcg atatccgcta tcttgaagcc ttccctgaaa taatatccct
     3361 cacggataag acctcgtatg atacctgtaa ttgtagcatt taccggaaca tattcagaca
     3421 atgcattgtc gtagctttct ttatccggat atatccgcgc tatctcatct cccttttgca
     3481 cgatgtctcc gatttttctg acatcatgaa tatatcctgc cgccggagcg tgaatcacac
     3541 gctcacttgt gaagccggcg atatttcccg gaacacctgt gttgggccgt gccatgccat
     3601 cggtaatgat gcgcgccaga ttgtgcccac gcatggactc aatcaccaga tgcacgtctt
     3661 gccctgccgt aaagcccggg cctactccta tcacaagcgg ggccatattg attgtggtac
     3721 ccagattttt ctttgctatt atggcatcta ccacaacatc cggctttaaa agtgcaattg
     3781 actcaccctt tgggtctata agaagcggga cctctccggc ttcaagcaca gcttcgatgg
     3841 ctgaactatc ccacttttct gatgcaatat gagctgccgg tgtgtcattt atccctgaat
     3901 atgtctctgt atcagtctct gcatcagcca aggcaggaac aagtctggct gtcaccccct
     3961 ctacagctgc gctcccatca tacactgcct cgcaaaagga cacctggcgc cgtatggcag
     4021 ccggatagtc tgtctcaagc gctatcaccc tatgccctgc ccggtgcagt ctgtggatga
     4081 tgccggtcgc aagatctcct gcaccgcggc atatgattaa caaatttttc tttattatct
     4141 gattattgct ctgattcatg ctcatctgat taattaccct gttaccttat tatttccata
     4201 tttgcaacta ttcagaacca cccacgctca ttcgcaaaaa cgcacgatac tcgtccttca
     4261 aaaccgctat cgcgcagctt tctgacatag acctgccgca gcgtatctat aatcgaatca
     4321 tcaaccactg catccgctgc cgttccaagc tcctcttgca taagctcata tcgaaataca
     4381 acgtccttac atgacctgcc cttttcccat gtgccaagca ctatgataac atctgtggta
     4441 cccggatata tgacaggctc atccgctgcc ggatatttga gggaatggtg ttttgcccca
     4501 tctgcttcga ttataacata atctaacgcc tgtggcatgt ctttaatcag tttatcaagc
     4561 aaatcatacg gcagtccgca catctttgac tttgactgct ctgatatttt ctgttcagat
     4621 attttctgcc ccgccataca atatccatct tttattattt tatcccttaa cgcaaaaaaa
     4681 tcgcaggata ggtcagtgtc agcttcgaca tacatgtgcg ttgttgttgt gacaagcaca
     4741 ccttttccac tcctgtgata ttcccttgca agcttatgaa cgagtgtggt cttgccacct
     4801 gcgcccacta ttgaaatcac tttttccata tatttccata ctttcccaca aacaataaag
     4861 ccgccggtgt acttaatgca ccggacggct tcaaaatcct aagcgttagt catccacacg
     4921 cttcacatcg tctttctcgt ccatatccca catatatggc gcgttctttg acttcttcgg
     4981 caaaataata ttgagaagaa tagcgcaaag tgagcaaggc acgatacctg aaccgccaaa
     5041 tataagctgt acatagtatg gcagatgtga tgtagcaccg gttgtagcgc caagtccgta
     5101 gcctaaaccg attgctactg agataattgt aatctcacgg ctgccgattg gctcctttgt
     5161 gatgagctca attcccgaaa tcaggataga ggcaaacatc ataacagcag ctccgccgag
     5221 cactgactga ggcatgattg acatgagagc tccaatcttt gggcagaagc cgcaaataac
     5281 gaggaatata gctcccattg aaatagcaaa tctattgaca acctttgtca ttgaaacaag
     5341 acctacattc tgtgagaatg aggtatttgg cagtactccg aaaagcgctg caaatgatga
     5401 accaagacca tcacagataa caccgcctga aagctctgaa tctgtagcct ctctgtccat
     5461 tccaccctcc acacaggctg agatatcgcc aactgtctcg attgcagtaa cgataaacat
     5521 gatgccgatt gggatgattg cattggcatg gaaggaaatt cccacatctg acagctttcc
     5581 aaagcctata aagcttggaa ctgctatcca tgccgcattt ttaacctcat caaagtttac
     5641 aacccatgag tatgtgtatt taacaccgtc agcggtcaca cctgtgtgtg acatggtaag
     5701 tgtcataatg attgctacta catatcctgc caggatacca aaaaggatgg cagatgatga
     5761 aaaaatactg cgcgatggtg caaaatgctt aagaagcacg attactataa gtacaaccat
     5821 tccaataaaa aggtttggca gtgagccgta atcatttttg cctgttccac cacagaagta
     5881 gttgattccc actccaagga gtgaaagtcc gattgctata acaactgagc ctgtaacgat
     5941 tgccgggaaa aacttgcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccca taatagctcc
     6061 atatgctata actccgccgc cacacgctgc aactgtatta tttaatactc caagaaagcc
     6121 cgaagaggtt cccataacga taggtacctt tccgccgaca ggtccgatgg aaaacatctg
     6181 tactaatgtt acaatacctg cgataatcat tgcattctgt aagagagcag ttctaagctc
     6241 cgcaaagcca gcatcagctg tgagcccgca tgctcccata acaatgataa gaggtgtaag
     6301 gtttccgatg aacatagcca taacatgctg cagtccaagc ggaatagcct gagacagagg
     6361 tattctgccc tcaaactggt aaggtgaaga gttcgttgtc tttttcattg gttcctccaa
     6421 ataattatac tcttaattta ataaaaaatt tacaaaatta ttttacgagc ataaggctat
     6481 gtggtcaatt tagtattact tataatataa tgtccattta cttataaata aatgctattg
     6541 aaattagttt ctgacaacaa cagggcacac cacaaccggt atgccctatc tattacaaac
     6601 gatatagcta ttcagaataa tctaaattta ttcaacccta agcagctcgc agtttccctg
     6661 catatccttc cagaaatcct ttataggtgt gccgcgaaac tgattgacta tgctgcagtt
     6721 cattgcaccg tgtcccacga caagcacatt tttgcctgcc tcaagctgcg gtctcagcac
     6781 ctgctctata aactcgcctg ttctgtgata tagctcctca aagctctctg cgccgtcctt
     6841 tgccacatat ttttccggct cctcaaagag caggtagaca gggcattcnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnc agtagcatat gtcaaagtct atatccttat atttttctgc
     7081 ggcatcgtaa gccatctgcc tgccggtttc gttgagtggg atatccacct ccccctgcag
     7141 tctgtgctcc tcattccaat ctgttcttcc atgtcgcaaa atataaaaca taattaatcc
     7201 tcgatttctc catttctatg tctctcgaag aaatgccttg tctccttaac aacaacacca
     7261 ctaagagcaa acagcgctat catatttggc agagccatga gaccgttgaa gatatcagcg
     7321 atagtccaca ccgcacttac agtcatataa ggtccgatga aaactgcaag tatatatatc
     7381 cagcgataaa cctttacata tttcatcctt ccgccactca aatactctaa gcaccgctca
     7441 ctataataat cccaaccgag tatagttgta aaggcaaaaa atacaagaca cagcataagt
     7501 acaaatgctg atagctcttt cggtaaagga agaccattct ggaatgcgta tgtagtaacc
     7561 tgcacaccct cgagtccgtc aacctgccat gcgcctgtga gcacgatgga aagtcctgta
     7621 agtgtacata taactattgt atcaatgaag gttcctgtca tgctgacaag gccctgacgt
     7681 acaggctcct ttgtctgggc tgctgccgct gcaatcgggg cactaccaag tcctgcctcg
     7741 tttgaaaaga ttcctctggc aacacccttt tgcattgcaa caagaaagct tcccacggca
     7801 ccgcctgtta tggccttcgg tgaaaacgct gcctttacta ttgtagcaaa cgctgccgga
     7861 accgctgtga tattgcatac cacaagcacg agcacaaata caatatatat aacagccatg
     7921 aaaggaacta ttatctggct gacacttgca atacgcttta ctccgccgat aagcactgct
     7981 gccacgcaga atgccagtac aagcgaagca atgacaactg accatgaata ctcaccaagg
     8041 aacggtaaaa ccttcactgt atgctggttt ttagggtcaa aaaagttgtt tacagcactt
     8101 gatattccgt ttacctggct gaatgttcct attccgaaaa gtccaacgca tacaccgaaa
     8161 aatgcaaata tctttgcaag ccatttccat tttgcaccca ttccctgctc tatgtagtag
     8221 aatggtccgc cgagactatg tccgtccttg ccgatgacac gatattttac agccaaaagt
     8281 ccctctgaat actttgttgc catgccaaag aaggctgcaa gcaccatcca gaaaagtgct
     8341 cccggtcctc ctgcaccgac tgcagttgcc acaccgacta tgttgccggt tccgattgtt
     8401 gcgctaagcg cggtacagag tgcgccaaag ctcgtaatct cgccatcgcc gccctcttca
     8461 tttttgacca tccactttag tgccagatga agctttctca tctgaagaag ccccagacga
     8521 actgtgagca ttatacctcc tgccatgatg agcaccatga gcggcactcc ccagacgaaa
     8581 tcgtccactg ctttaagaaa aatgttgatt gtgttccaca ttgaaatact cctgcctctc
     8641 ttttttatct aaacacaaag tctttgtgtt ttgagcaaat actattaata taaataattt
     8701 catccggaaa caattgaaaa agcaggctta gcagcactta cgcgaattga aataaaaaac
     8761 cccatatacg ctgcatatac agtctgatac atgggattaa gaagattatc ttaagaatgt
     8821 ctgtccaata tcttctccgt cctgttgcct gagagattcg tacgggaagc agcctgtgcc
     8881 atgatatatt catagtcact gcactcctac tgcttcctat tcgtacgttg caccttcggc
     8941 ccccgccaag cgggtgtctc cagagagtcg atccatttac agttgcaccg cctgctgcgg
     9001 cacctgagag ttttactcct tcggtaggct ttgataaggt aaaatcgctg ccgttttcct
     9061 gcaaacttca tctcgaaatt gtttatttta aatttattgt ttttattaat cgttaagaat
     9121 tatatgacat atatacgatt taatcaatag cacaaatgta ctaaactgtc tcaaccttaa
     9181 tcacattggt gttgcctgtc ttaccgttga tgagacctcc tgtgagcact atatgatcac
     9241 cctttttgag cttcatgaca ctctttgcct cgttcatcgc attgtagaac ataacctcca
     9301 ttgagttgta ttcctc
//
LOCUS       DPUF01000070           12560 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10306, whole
            genome shotgun sequence.
ACCESSION   DPUF01000070 DPUF01000000
VERSION     DPUF01000070.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 12560)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12560)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12560
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10306"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="DHV81_02495"
     CDS             complement(<1..410)
                     /locus_tag="DHV81_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCI93390.1"
                     /translation="MAIIFNPNKKIFTLQTAHTTYQMQVDRLGYLLHLYYGAKSTCDM
                     DYVLTYADRGFSGNPYAAGMNRTYSLDTLPQEYPTLGTGDFRNIALDIKNEQGTESVE
                     LLYKSYEIRDGKYALKGLPAVWASDDEAQTLEIVL"
     gene            complement(450..1286)
                     /locus_tag="DHV81_02500"
     CDS             complement(450..1286)
                     /locus_tag="DHV81_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI93391.1"
                     /translation="MKKQKTNWVLTIVLILGTLTVFFPLYMAVIIAFKKPSEMTNDVA
                     GALSFPSKWSFSNFTEAMKVTDFWHSLGNSLLITIVTIVLAILIHSIAGYVIGRGMAR
                     KKSFRFIYLYIVSGMFVPFSILMMPLVKQTAQMGLGNRAGVIILYLVFYMPMNVLLYS
                     GYLKNIPMALEEAADIDGASTWTTYWRVVFPMMKPMHATVAILTALGTWNDVMTPLVI
                     MSGTGQNTLPLAQLNFQTQFGTNYNLAFASYILALIPILIFYIICQKQILNGVANGAI
                     KG"
     gene            complement(1286..2161)
                     /locus_tag="DHV81_02505"
     CDS             complement(1286..2161)
                     /locus_tag="DHV81_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI93392.1"
                     /translation="MKKKMSNRDKTFLAVVIPVVVLFFAFNTLPMIKGVIYSFTNYKG
                     YGSYDYVGLRNYTDLFTDARVGKSYLFTFKYAIAGTVLVNVLSLVMAVALNSNIRGKS
                     ALRGVYFVPNILGGLVIGYIFSFIFTYILPVVGNTFNIGFLQNSILASEKYAWIGVLI
                     VGVWQAVAMNTIIYISGLQTVPEDVYEASMLDGAGKWKQFWKVTFPLVMPFVTINLVL
                     STKNFLMVFDQIMSLTKGGPAQSTESISYLIYRNGMDGGQFGFQSANAVIFFVVIVAI
                     SLFQMGVMNKKEEQL"
     gene            complement(2158..3459)
                     /locus_tag="DHV81_02510"
     CDS             complement(2158..3459)
                     /locus_tag="DHV81_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCI93393.1"
                     /translation="MAKRKICQAMAVVLALGMCTTGLTGCGNEKRADGKTEIEVVSYK
                     PEAVKAFEKIEKQFNETHDDIHLTISSPNEAMTILKTRFIREDYPDIIAIGGDINYSN
                     FLDADLFEDISDLDAVDTVKKAYLDMDKELEFIPKDGTYALPYAANAAGVLYNKDMFA
                     ENGWKVPTTWSEFTALCNEIKESGTLPLYLGFKDTWTCLAPWNALAVGLCDSDTCNQV
                     NMGNTTFEEAYSPVADKIRTLLDYAEDNPYAYSYNDACTAFARGESAMYTIGSYAIPQ
                     IKSVNPDMNIGSFTFPANDNEADNVLNSGIDLQFSVMKACKNKEAAYEVLEYLYSDET
                     IQTYLDDQGGIACKDGDFAIPDTLEDMQEYIKDNRMSDYQDHHYPSEMSVDAMIQTYL
                     LDTGDNAKEKFLKKFDSDWKRYNRDLIREVQDYQKEQEDAK"
     gene            3648..4490
                     /locus_tag="DHV81_02515"
     CDS             3648..4490
                     /locus_tag="DHV81_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI93394.1"
                     /translation="MDKLSFNIFPNVNFIDLCMYQFGHEQCEPGHSFGPATRNHYLFH
                     YVLSGTGTLMADNAKGITQTYNIKSGQGFLIFPGQINTYIADDKLPWEYIWIEFDGLR
                     VKEALTTTELTKNNPVYHTHSKDIREKLVEEMLYITRHPSESPYHLIGHMYLFLDYLM
                     QSAKSSKLVPGGKMSDFYIKEAMNYIEQNFQNDISIEDIARVCGINRSYLGKIFKNSV
                     GHSPQEFLMNYRMIKATELLKLTSLSIADVGSAVGYENQLHFSRAFKNIYGVAPREWR
                     NEHK"
     gene            complement(4493..>5096)
                     /locus_tag="DHV81_02520"
     CDS             complement(4493..>5096)
                     /locus_tag="DHV81_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI93395.1"
                     /translation="AKQSGQMQGELIADYIKNNPGVDKNGDGRIQYVILEGEMGHQDA
                     IVRTESVTESMKNNGLQIEKLSCQIANWNRAQAQNRMTQLIGQYKNSIELVIANNDAM
                     ALGAIDAYEKLGVTESNVPAFFGVDGTDDGLEAVQQSKLAATVYNDKEGQARAMAQLA
                     YLAATGGSMKNIKFEDKKYVYLPYEKVTPDNVNEFVKDEQ"
     assembly_gap    5097..5194
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5195..5547)
                     /locus_tag="DHV81_02525"
     CDS             complement(<5195..5547)
                     /locus_tag="DHV81_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI93396.1"
                     /translation="MRSRIRNTMMILCFALILSFVSCSNTRVDEKKQIYIGVTCYDQK
                     DTFIGELIETFKKECASLDTDKYDISMTIMDAAGSQRAQDDQVQEMIEDGCNVLCINL
                     ADRTDLSHIINAAME"
     gene            complement(5562..6617)
                     /locus_tag="DHV81_02530"
     CDS             complement(5562..6617)
                     /locus_tag="DHV81_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCI93397.1"
                     /translation="MKQVQLPCCMGGVRVTVKNKWTNMSKNKRNFIITELLLGVLLVI
                     GIGFIAYNKLNEKPERIAVVMADVDESHSAAFKYGMKMAASEYGVDVVMISKENLNNM
                     SDEIDVIKQEINKKADAVVFKPTAEKMTEEKSLNTLIRINKKTPIMVVGDQAGSESFK
                     SLNTVEFDQYSMGAKLAQKLLADNNGTLSGKKIGIYCENTESDACKKRIDGIKDTLAE
                     SGCVVMWIVSGVSDTGEKINLQSQRKVDIVLAIDDASVVDAAKEASDNNLQGALVYGI
                     GNSTEAIYYLDSGWTEALITPDEFAAGYKSVVGLVDMLRGSRKYSVEEKPVSYQLLTR
                     KNLFDEENKKLLYAISQ"
     gene            complement(6614..8407)
                     /locus_tag="DHV81_02535"
     CDS             complement(6614..8407)
                     /locus_tag="DHV81_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCI93398.1"
                     /translation="MRRIRYKSGNIQSIIMLSFSLLSLAIMLVTVVVMYIKFADASQD
                     SIIESNHKVMDQTIDSVESYLVNMRQVSDSAYYNVIKENDILKQSDSIHDGMSLLYES
                     NKEYLRSIALYNQYGSLIAAEPVVSQKEDPNVTKQDWFIEAMERMENIHFSTPHVQNL
                     FDDGSMRYHLVISSSRAVELTSGSESQMGVMLVDMDYSSVSRMLERINTSGKGQYYYL
                     CDAKGNIIYHPHQTQFDGDVPENSEVAAKSQNSIYDDYLNGVHRKIMVDTISYTGWKL
                     VCVMPYSIFTNKMADVKQFVFVIMIIMAMMFVWINRVIAIRISKPIMKLDDSVKRYEN
                     GNEADIYVGGSSEIRHLGYSIRNSYKQNNELMKKIVWEQNERRKSELDVLQSQINPHF
                     LYNTLDSITWMIEGGKNEEASFMITQLAKLFRISLSKGHTIIPVRDELLHAKSYMNIQ
                     KVRYKNKFEVSFDVDEEIMDYCAVKLVLQPILENALNYGIRELDDFGEIVVGGQKVGE
                     DIIITVSDNGMGIPENEIPLLLTNTDRVHKKGSGVGLVNVNNRIKILFGERYGLHIES
                     ELDEGTVVTIKIPAIVYSEENRLQFEEHHIA"
     gene            complement(8388..9992)
                     /locus_tag="DHV81_02540"
     CDS             complement(8388..9992)
                     /locus_tag="DHV81_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93399.1"
                     /translation="MYTLLLVDDEEDVIEVIERKVSWEQIGFHVVGHAGNGFKALEMM
                     EDMQPDVVMTDIRMPYMDGLELCSNIRQRFPATKLLLFTGFDDFEYAKEAVHLEIEEY
                     ILKPLNLAEITEVFKKLKTKLDDELNEKKNTDILKQYYAASLPVLQSNFYTTLIEGRI
                     PENELGRYMRDYKIVLEGPYYCCIIIHTSASQMPQGMDIRLLAVSVERQAQADLKERW
                     NGRIFNYLGDTVMIAQLVQQEDISELTDECDRFCKYVNHVMGAKVTVGIGQVCENVQE
                     LVSSYQSAREAVSYRVLYGSNRAINMTEVEPQRRISKDGDEGNELSYLFKMICIGKIE
                     DVGQAVEAYMQHNFMSQQSLENYHVAVMELISELYHFMSNNELNAQEISGSVGRLYNE
                     LSNFEPVVLKQWLLDFSSRLHDDMADARYNSKKSLIDSAKDYVHRNYRSVDLGLDDTC
                     KELGVSNSYFSSLFKKETGSSFVEYLTDYRMDKAARMLVETDDKSYVIAQNVGYADPN
                     YFSYVFKRKYGVSPSRYRTEYEKNKV"
     gene            complement(10281..11930)
                     /locus_tag="DHV81_02545"
     CDS             complement(10281..11930)
                     /locus_tag="DHV81_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside ABC transporter permease"
                     /protein_id="HCI93400.1"
                     /translation="MANSKILTAEQERTLRQPIDEYVGGIQKEIDALRKDGTTKVVEC
                     QSAIAGIKRDKTLSKGEKESEIAACEKELAKAKAVEAKNKDEISKLIAKAESYLKENF
                     DSKYYNAVKASCEAEKAEALAAHNERMAELDKKHKAALAKTSDRTEIKEENYVHKNRI
                     SNEKLELEKEYQRIKDRKHEAYSYKYHLIDMLRLSKFTFMETRAQKWENYKYTFNRRN
                     FLLQNGLYIAIILIFIALCIITPIKKGTPLLTYNNILNILQQASPRMFLALGVAGLIL
                     LTGTDLSVGRMVGMGMTTATIIMHQGINTGSVFGHIFDFTGVPTGARVVIALLACIVL
                     CTFFTSIAGFFTAKFKMHPFISTMANMLVIFGIVTYATKGVSFGAIEPVIPNMIIPKL
                     NGFPTIILWAIAAIAIVWFIWNKTTFGKNLYAVGGNPEAAAVSGISVFAVTLGAFIMA
                     GILYGFGSWLECARMVGSGSAAYGQGWDMDAIAACVVGGVSFTGGIGKISGVVTGVCI
                     FTALTYSLTILGIDTNLQFVFSGIIILVAVTLDCLKYVQKK"
     gene            complement(11949..>12560)
                     /locus_tag="DHV81_02550"
     CDS             complement(11949..>12560)
                     /locus_tag="DHV81_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCI93401.1"
                     /translation="TELLETIFGIRTRAAGRVYFKNQLMNFTCAKEAMDYGFALITEE
                     RKANGLFLKGNLTFNTTIANLGSYKKGPALSDSKMTKATSDEIKIMHTKCMGPEDMIS
                     ALSGGNQQKVIFGKWLERGPQIFMMDEPTRGIDVGAKYEIYELIINMAKQGKTVIVVS
                     SEMPEILGITNRIGVMSNGRLSGIVNTKETNQEELLRLSAKYL"
BASE COUNT         3309 a         3064 c         2195 g         3894 t
ORIGIN      
        1 agaactattt caagagtctg cgcctcgtca tcagatgccc acactgccgg aagtcccttt
       61 agagcgtatt ttccatctct gatttcataa ctcttataaa gaagctcaac actctcagtg
      121 ccctgttcgt ttttgatatc aagcgcaata tttctgaaat cgcctgtgcc aagtgtcgga
      181 tactcctgtg gcagcgtatc aagcgaatat gtacggttca tgcctgctgc gtaaggattg
      241 cctgaaaagc ctctgtcagc atatgtcaga acatagtcca tatcacatgt gctttttgct
      301 ccgtagtaca gatgcagcag atagccaagt ctgtccacct gcatctgata tgtggtgtga
      361 gctgtctgta aagtaaaaat tttcttgttt ggattaaata taattgccat aaaatcactc
      421 catatcattg ttaaaatagt gtttaaaaat tatcccttga ttgcaccgtt tgcaacaccg
      481 tttaatatct gcttctggca gattatatag aatataagta tcggtatcaa agccagaata
      541 tatgatgcaa atgccagatt gtagttggtt ccaaactgtg tctggaaatt cagctgtgcc
      601 agcggcagtg tattctgtcc tgttcctgac atgatgacaa gcggtgtcat gacatcattc
      661 caggtaccga gtgctgtcag gatggctact gtcgcatgca taggcttcat cataggaaat
      721 acaactctcc agtatgttgt ccatgtgctt gcgccatcga tgtctgcagc ctcctcaagt
      781 gccataggga tatttttcaa gtatccacta tataataata cattcattgg catgtaaaat
      841 acaagatata aaatgataac tcctgccctg tttccaagtc ccatctgcgc agtctgcttt
      901 acaagaggca tcatgagaat tgagaatggc acaaacatac cgcttacaat gtaaagatat
      961 atgaaacgga agctcttttt ccttgccatg cctcgtccga taacatatcc tgcaatcgaa
     1021 tggataagga ttgcgagaac aatggtcaca atagtgataa gtaagctgtt tccgagagaa
     1081 tgccagaaat cagtcacctt catagcctct gtgaagtttg aaaagctcca ctttgaaggg
     1141 aatgaaagag ctcctgcgac atcatttgtc atctcactcg gtttcttgaa ggcaatgata
     1201 acagccatat acagtgggaa aaatacggtt aaggttccca atattaaaac gatggtaagt
     1261 acccagtttg ttttttgttt tttcattata actgctcctc cttcttattc ataacgccca
     1321 tctggaatag tgaaatagcc acaattacaa caaagaaaat tactgcgttt gcactctgga
     1381 agccaaactg tcctccatcc ataccatttc tgtaaatcag gtatgagatt gactcagtgc
     1441 tctgcgccgg gccgcccttt gtcaatgaca taatctggtc aaataccatg aggaagttct
     1501 ttgtgctgag caccagattg attgtaacaa atggcataac gagtggaaat gtaaccttcc
     1561 agaactgctt ccatttgcct gctccgtcga gcatgctggc ttcgtaaaca tcctcaggca
     1621 ctgtctgcag tccggatata taaatgatgg tattcatggc aaccgcctgc cataccccga
     1681 caatcagcac accaatccat gcatattttt cacttgccag aatactgttt tgtaggaaac
     1741 cgatgttgaa ggtatttcct actaccggca gaatatatgt aaaaataaag ctgaaaatgt
     1801 aaccaataac cagtccgcca aggatattcg gtacgaaata aacacctctt aatgcactct
     1861 tgccgcggat attgctgttt aacgcaactg ccattacaag gcttaataca tttacaagta
     1921 ctgttcctgc aatcgcatat ttaaaggtaa acagatagct ctttccgact ctggcgtcgg
     1981 taaacaggtc tgtatagttt ctaagtccga cataatcata tgagccatag cccttgtagt
     2041 ttgtaaagct gtaaatcaca ccctttatca tcggcagtgt gttgaatgcg aaaaataaaa
     2101 ccacaacagg tatcacaact gccaagaatg tcttgtccct atttgacatt ttctttttca
     2161 ttttgcatcc tcctgttctt tctggtaatc ctgtacttca cgaatcagat ctctgttgta
     2221 acgcttccag tctgaatcaa atttctttaa gaacttctcc tttgcattgt cgcctgtgtc
     2281 aagcagatat gtctgaatca tggcgtccac gctcatctca cttggataat gatgatcctg
     2341 ataatctgac atacggttgt cctttatata ctcctgcata tcctccagtg tatccggtat
     2401 tgcgaaatct ccatccttgc aggcaattcc gccctggtca tccagatatg tctgaattgt
     2461 ctcatcgctg tacagatatt caagcacctc gtatgctgct tccttatttt tgcaggcctt
     2521 cataactgaa aactgtaaat ctattcctga atttaacaca ttgtcagcct cattatcatt
     2581 tgccgggaaa gtaaatgaac cgatattcat atcaggatta accgacttaa tctgaggaat
     2641 agcatagctt cctatcgtgt acatggcaga ttcacctctg gcaaaggctg tacatgcgtc
     2701 attgtagctg taagcatatg gattgtcctc tgcataatca agcaatgtgc gtatcttgtc
     2761 ggcaaccgga gaatatgcct cctcaaatgt cgtatttccc atatttacct gattacaggt
     2821 gtctgaatca caaaggccta ctgcaagggc attccacggt gccagacagg tccatgtatc
     2881 cttaaagccc agatacaaag gaagtgttcc gctttccttt atctcattgc acagtgctgt
     2941 aaactcactc caggttgtag gcaccttcca gccattctcc gcaaacatat ccttgttgta
     3001 aagcactcct gctgcatttg cggcgtaagg aagtgcatag gtgccatcct ttggaatgaa
     3061 ctcaagctcc ttgtccatat ccagatatgc cttttttaca gtgtccactg catccagatc
     3121 tgagatatcc tcaaacaaat ctgcatccag gaaatttgaa taattgatat ctccacctat
     3181 tgcaatgatg tccggataat cctcacggat aaagcgtgtc tttaaaatag tcatagcctc
     3241 attcggagat gagatagtca gatgaatatc atcgtgagtc tcattaaatt gcttctcaat
     3301 cttttcgaaa gccttaacag cctccggctt ataagaaacc acctcaatct cagtttttcc
     3361 gtccgcccgc ttttcatttc cacatccggt cagtcctgta gtgcacattc cgagtgccag
     3421 cacaaccgcc atagcctgac aaatctttct ttttgccata ttttttcact ccttttctgt
     3481 tttatttgct ctttttcctg ataaactgat tataacatac cattatgcat acaataaata
     3541 cccttatttt tgctttttta tatcaatatg ctataaaata tgctataatt gcatataatg
     3601 tcgcataagg gtatatttat agtattaatc aataaggagt gactaacatg gacaagcttt
     3661 ccttcaatat ttttccaaat gtaaatttca tagatttgtg tatgtaccag ttcggccacg
     3721 agcagtgcga accgggacac tcatttggtc ctgccacaag aaaccactat ctattccact
     3781 atgtactgtc cggcaccggc actctgatgg ccgataatgc aaagggcatt acacaaacct
     3841 acaacataaa aagcgggcag ggctttctga tttttcccgg ccagataaat acctatatcg
     3901 cagatgacaa acttccctgg gaatacatat ggattgagtt tgacggtctg cgcgtcaagg
     3961 aggctctcac cacaacagag cttaccaaaa ataatcctgt atatcacaca cactcaaagg
     4021 atatccgcga aaagcttgtt gaggaaatgc tctacatcac aaggcacccc tcagaatcac
     4081 catatcacct gattgggcat atgtaccttt ttctggatta tctgatgcag tcagccaaat
     4141 catcaaaact cgttccaggt gggaaaatga gtgattttta tataaaagaa gcgatgaatt
     4201 atatcgagca gaattttcag aatgatatat caatcgaaga tattgccaga gtctgcggca
     4261 taaaccgaag ctatctgggc aaaattttca aaaattccgt tggacattca cctcaggaat
     4321 ttctcatgaa ttatcgtatg ataaaggcca cagagctttt aaagctgacc tccttatcca
     4381 tcgcagatgt tggttctgcc gttggatacg aaaatcagct tcatttctca cgcgccttta
     4441 aaaatatcta cggagtcgct ccgagggagt ggaggaacga gcataaataa gctcactgtt
     4501 catccttcac aaattcattt acattgtcag gtgtcacctt ctcatacggc agataaacat
     4561 actttttatc ctcaaacttt atgtttttca tgctgccccc ggtggctgcc agataagcaa
     4621 gctgagccat agcccttgcc tgtccctcct tgtcattgta gactgttgcc gcaagcttcg
     4681 actgctgcac tgcttcaagc ccatcgtctg tgccgtccac tccaaaaaat gcaggtacat
     4741 tcgactccgt cacaccaagc ttttcgtatg cgtctatcgc tccgagtgcc attgcatcgt
     4801 tgtttgcaat aaccagctct atgctgtttt tatactgacc tataagctgt gtcatacggt
     4861 tctgcgcctg agcacgattc cagtttgcta tctgacagct cagcttttct atctgaaggc
     4921 cgttattctt catgctctct gtgacactct ctgtcctgac gatggcatcc tgatgtccca
     4981 tctctccctc taggatgacg tactgtatcc tgccatcacc gtttttatcc acacccggat
     5041 tattctttat ataatcggca atgagttccc cctgcatctg acctgactgc tttgccnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttctcc attgcagcat ttataatatg
     5221 cgacaaatct gttctgtctg ccagattaat acacagcaca ttgcagccgt cctctatcat
     5281 ctcctgcacc tggtcatcct gtgcacgctg tgagcctgca gcatccatga ttgtcatgct
     5341 gatatcgtat ttgtctgtat caagacttgc gcactccttt ttgaaagttt ctatcagctc
     5401 cccgataaat gtatccttct ggtcataaca ggtcacacct atatatatct gttttttctc
     5461 gtcaactctt gtgttgctgc acgagacaaa tgacaatatc agtgcaaaac acagtatcat
     5521 catggtattt cttattctgc ttctcattta ttgttccccc actactggct gattgcatac
     5581 aaaagcttct tgttttcttc atcaaacaga ttttttctgg taagaagctg gtaagatacc
     5641 ggcttttcct ctactgaata ttttctactg ccacgcagca tatccaccag tcctacaaca
     5701 cttttgtatc cggcagcaaa ttcatcaggt gtgataagag cctctgtcca gcccgaatcg
     5761 agataatata tggcttctgt tgagtttcct attccatata ccagtgcacc ctgcagatta
     5821 ttatcagagg cttcctttgc cgcatcaacc acacttgcat catctattgc aagaactatg
     5881 tcaacctttc tctgtgactg cagatttatt ttctccccgg tatccgaaac acctgataca
     5941 atccacatca caacacagcc gctctccgcg agagtatcct ttatgccatc aatgcgtttt
     6001 ttacaggcat cggattcagt gttttcacaa tagattccta tttttttacc tgaaagtgtt
     6061 ccgttgttgt cagcaagaag cttttgtgcc agtttggcac ccatgctgta ctggtcaaac
     6121 tccactgtat ttaacgactt aaagctctcg cttcccgcct ggtctccgac taccattatc
     6181 ggtgtctttt tatttatccg gataagtgta ttaaggcttt tttcttctgt cattttttct
     6241 gctgtcggct tgaaaacgac cgcatcagct tttttgttta tctcctgctt tatgacatca
     6301 atttcatctg acatgttgtt cagattttcc ttacttatca taactacatc cacaccatac
     6361 tcagaggccg ccattttcat gccatattta aacgcagcac tgtggctttc atccacatca
     6421 gccatcacaa ctgcaatacg ctccggtttt tcattgagct tattgtatgc gataaaacct
     6481 attcctatca caagtagtac tcccagcagc agctctgtta tgatgaagtt tctcttattc
     6541 ttgctcatat ttgtccattt atttttcact gtcactctca cacctcccat gcagcaaggc
     6601 aattgtactt gcttcatgct atatgatgct cctcaaattg aagtctgttt tcctctgaat
     6661 agacaattgc cgggattttg attgtcacaa ctgttccctc atcaagctca ctttctatat
     6721 gcaggccata gcgctctcca aacagtatct ttatcctgtt atttacattg acaagcccca
     6781 ctcccgagcc cttcttgtga accctgtccg tatttgtcag caaaagtgga atttcattct
     6841 ccggaatgcc cattccgttg tctgataccg ttatgatgat atcctctccc actttttggc
     6901 ctccgactac tatctctccg aaatcgtcca gctctctgat tccgtaattt agtgcgtttt
     6961 ccaaaatcgg ctgaagcacc agcttcaccg cacagtagtc cattatttcc tcgtctacat
     7021 caaagctcac ctcaaatttg ttcttatagc gcaccttctg aatattcatg tagctttttg
     7081 cgtgcaaaag ctcatctcgt acaggtatta tggtatgtcc cttcgacaga ctgatacgga
     7141 aaagttttgc aagctgcgta atcataaacg aggcctcctc gtttttgcct ccttcaatca
     7201 tccatgtgat ggagtcaaga gtattataca aaaagtgcgg attaatctgg ctttgaagca
     7261 catcaagctc gctcttccgt ctctcgttct gctcccatac gatctttttc atgagctcat
     7321 tgttctgctt atatgaatta cgtatagagt atcccagatg ccgtatctct gaggaaccac
     7381 ctacataaat atctgcttca ttgccattct catacctttt cacagaatca tccagcttca
     7441 ttatcggctt tgaaatacgt atagctatca ctctgtttat ccacacaaac atcatagcca
     7501 taataatcat tataacaaaa acaaactgct tcacatccgc catcttattg gtaaagatgg
     7561 agtatggcat cacacataca agcttccagc ctgtgtagct gattgtatct accataatct
     7621 tacgatgcac tccattcaga taatcatcat atatactgtt ttgcgacttt gccgcaacct
     7681 cgctgttttc cggaacatct ccatcaaact gtgtctgatg cggatggtaa ataatattgc
     7741 cctttgcatc acacagataa taatactgtc cttttcccga tgtatttatc ctctcaagca
     7801 tccttgatac actcgaataa tccatgtcca caagcatcac acccatctgt gattctgaac
     7861 cggaggtcag ctccacggca cggcttgatg atatgaccag atgatatctc atggaaccat
     7921 cgtcaaacag attctgtaca tgaggtgttg aaaaatgtat attttccatg cgctccatag
     7981 cttctatgaa ccagtcctgt tttgtcacat tcgggtcctc cttctgggac acaacaggct
     8041 ctgcagctat aaggcttcca tactgattat acaaggcaat actgcgcaga tattccttgt
     8101 ttgactcata gagcaggctc atgccatcgt gtatgctgtc agactgcttt aaaatatcat
     8161 tctcctttat tacattataa tacgccgaat ccgaaacctg cctcatattg accagatagc
     8221 tttccacact gtctattgtc tggtccatca ccttatgatt gctctctatg atactgtcct
     8281 gcgatgcatc ggcaaatttt atatacatca ccacaactgt gacaagcatt attgcaagcg
     8341 acagcagtga gaatgagagc attattatgg attgtatatt gcctgattta taccttattc
     8401 ttctcatact ctgtcctgta cctggatggg gaaaccccat actttctctt aaaaacataa
     8461 ctgaaatagt tcggatccgc atagcctaca ttctgggcta tgacatagct cttgtcgtct
     8521 gtctccacaa gcattctggc agccttgtcc attctgtaat cagtcagata ctccacaaac
     8581 gagctgccgg tttccttctt gaacagactt gaaaaatacg agttagatac tcctaactcc
     8641 ttacatgtat cgtcaagtcc aaggtccaca cttctgtaat tcctgtgcac ataatccttt
     8701 gcactgtcta tgagtgactt cttggagttg tatctggcat cagccatatc atcgtgcagc
     8761 ctgcttgaaa aatccaaaag ccactgcttg agcaccaccg gctcaaaatt ggagagctca
     8821 ttataaagcc tgcctacact tcccgatatt tcctgcgcat ttagctcatt attgctcatg
     8881 aagtgatata gctcactgat aagctccatt actgccacat ggtagttctc aagtgactgc
     8941 tgcgacataa aattgtgctg catatacgct tcaacagcct gtcccacatc ctctatcttg
     9001 ccgatacaaa tcattttaaa taaatatgac agctcatttc cctcatcgcc atcctttgat
     9061 atacgtcgct gtggctcaac ctctgtcatg tttattgccc tgttgctgcc ataaagcacc
     9121 ctgtaggata ctgcctccct cgcactctga taagagctca ccagctcctg tacattttcg
     9181 cacacctgtc cgattccgac cgtcaccttt gcccccatta catgatttac atatttgcag
     9241 aatctgtcgc actcatctgt cagctcagag atatcctcct gctgcacaag ctgggctatc
     9301 atgaccgtat caccgagata gttaaaaatc ctgccattcc agcgctcctt taaatcagcc
     9361 tgtgcctgtc gctctactga cactgcaagc agtctgatat ccatgccctg cggcatctga
     9421 cttgcggagg tatgtatgat aatacagcag taatatggtc cctcaagcac aatcttatag
     9481 tcacgcatgt acctgcccag ctcattttcc ggtatgcgtc cctcgataag tgtcgtgtaa
     9541 aaatttgact gcaaaacagg cagcgatgct gcataatact gcttcaatat gtctgtgttt
     9601 ttcttctcat tcagctcatc atccagcttt gtcttaagct ttttgaatac ctccgtgatt
     9661 tcagccagat tgagtggctt taaaatatac tcctctatct cgagatgcac agcctccttt
     9721 gcatactcaa aatcgtcgaa gcctgtgaaa agcaagagct tcgtcgcagg aaacctctgc
     9781 ctgatgttac tgcacagctc aagtccgtcc atgtaaggca tacgtatgtc agtcatcaca
     9841 acatcaggct gcatatcctc catcatctca agtgccttga agccattgcc cgcatgtcct
     9901 accacatgaa aaccaatctg ctcccatgat acctttctct cgattacctc tattacatcc
     9961 tcttcgtcat caaccagtaa taatgtatac atgttatgcc ttctccctgc tcataattat
    10021 tgtactgcat gcaccataaa gctcgtgtgc agcataaatt tatctcccgg ataacgtaat
    10081 ttattgggta actatccaga atagttactc tgtttaaact atattataac atacctgata
    10141 tattgttttg tagctttaca taaacaaaag tgtaattcta aaaagtataa ctgttcacac
    10201 cacaaactaa aagcagagat gccgccctga catctctgct aatcattttg gaagtatgaa
    10261 aaagaaattt tcttgcgata ttatttcttc tgaacgtatt tcagacagtc aagtgtaact
    10321 gctacaagaa tgataattcc tgagaatacg aactgaaggt ttgtatcaat accaaggatt
    10381 gtaagagagt atgtgagtgc tgtgaagata catacaccag ttacaacacc tgatatctta
    10441 ccgataccac ctgtgaatga tacaccacca actacacagg ctgcgattgc gtccatgtcc
    10501 cagccctgtc cataagctgc tgaacctgaa ccaaccattc ttgcacactc aagccatgaa
    10561 ccaaatccat agaggatacc tgccataatg aatgcgccga gagtaactgc aaatactgag
    10621 atacctgaaa ctgctgctgc ctccggatta ccaccgactg catagagatt tttaccaaat
    10681 gttgttttgt tccagataaa ccatacaatt gcgattgctg ctattgccca gaggatgatg
    10741 gttgggaaac cattcaactt tggaataatc atgttaggga tgaccggctc aattgcaccg
    10801 aatgaaacac cctttgtagc atatgttacg ataccgaaga taacaagcat gtttgccatg
    10861 gttgaaataa acggatgcat cttaaacttc gctgtaaaaa atcctgcaat acttgtgaag
    10921 aatgtacaaa gcacgataca tgcaagtaat gcgataacca ctcttgcacc tgtaggtact
    10981 cctgtaaagt caaatatatg accgaataca gagcctgtat tgataccctg atgcatgatg
    11041 attgtggctg ttgtcatacc cataccaacc attcgtccaa cagaaagatc tgttcctgtt
    11101 aagaggataa gtcctgcaac accaagtgct aagaacattc tcggtgaagc ctgctgaaga
    11161 atattcagta tgttgttgta tgtcagaaga ggtgtaccct tctttatagg tgttatgata
    11221 cacaatgcaa tgaatataag tataatcgca atatagagac cattctgtaa caggaagttt
    11281 cttctattga atgtatactt gtaattctcc catttttgtg cccttgtctc cataaatgtg
    11341 aactttgaaa gacgtaacat atcaatcaga tgatacttgt agctgtatgc ctcatgcttt
    11401 ctgtccttga ttctctgata ctctttctca agttcaagct tctcattgga aatacgattc
    11461 ttgtgtacgt aattttcttc ctttatctca gttctgtctg aggtctttgc cagagcagcc
    11521 ttatgctttt tatcaagctc tgccattctc tcattatgag cagcaagtgc ctctgccttt
    11581 tctgcctcac agcttgcttt aacagcattg taatactttg agtcaaagtt ttcctttaag
    11641 tagctttcag ccttggcgat gagcttagaa atctcatcct tgttctttgc ctcaacagcc
    11701 tttgcctttg caagctcttt ctcacatgct gctatttccg actctttctc accctttgaa
    11761 agagtcttat ctctcttgat gcctgctatc gctgactgac actctacaac ctttgtagtt
    11821 ccgtcttttc tgagtgcatc aatttctttt tgtattccac caacatattc gtcgataggc
    11881 tggcgcagag tgcgctcctg ctcagccgtt aaaatcttac tattagccat atctgtcttc
    11941 tccctttatt ataggtactt tgcactgagt ctcagcagtt cttcctgatt ggtttccttt
    12001 gtattgacaa ttcctgagag ccgtccgttt gacatgactc cgatacgatt tgttatacca
    12061 aggatctcag gcatctcaga tgaaactaca atgactgtct taccctgctt tgccatattg
    12121 attatcagct cgtaaatctc atatttggca cctacatcaa tacctcttgt aggctcatcc
    12181 atcatgaata tctgcggtcc tctttcaagc catttaccaa agataacctt ctgctggttt
    12241 cctcctgaga gagctgaaat catatcctcc ggtcccatac actttgtgtg cataatcttt
    12301 atctcatcag aggtggcctt tgtcatctta ctgtctgaaa gtgccggtcc cttcttatag
    12361 gaaccgagat ttgcgattgt cgtgttaaag gtgaggtttc ccttaaggaa aagtccattt
    12421 gcttttcttt cctctgtgat aagtgcgaag ccataatcca tagcctcctt ggcacaggtg
    12481 aaattcataa gctgattctt aaaatatact cgtcctgctg ctcttgtcct gattccaaaa
    12541 atcgtctcaa gaagctctgt
//
LOCUS       DPUF01000071           19464 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4716, whole
            genome shotgun sequence.
ACCESSION   DPUF01000071 DPUF01000000
VERSION     DPUF01000071.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 19464)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19464)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19464
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4716"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /locus_tag="DHV81_02555"
     CDS             complement(<1..1919)
                     /locus_tag="DHV81_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease"
                     /protein_id="HCI93402.1"
                     /translation="MKTKKFLSLVLSAAMILGVAVPVAAEPVSAGQGVEAEQQPIVEN
                     SITSGYILSDLDYNTPVYEPDEAVPYSDDWGYSADETIESKYPANGVSDIKAKYPSVR
                     NQNPLGTCWTFSSIGLAEFDLINDGAFDKNIDLSELHLAYYAYNSLLDPLGGTDGDYA
                     KYYMNNTSVQYGYLNRGGNYLMAARRMGQWCGPVSESDVPYSKVASNGYTASTIDTFL
                     NTGLSDEYAYSKDKAHLENTYMINIKENASDVKKAIKKYGAVGIMYSHNDNGYHYINN
                     SYNDKTNNRAGHAVMVVGWDDNYSKDNFRDGVKPEKDGAWLIRNSWGDGTGSYYNQSY
                     FWMSYETFSLSDTAWVFDFSANDGYDNNYQVDGGLNVAHQSGYRKLANVFTTQAKQGV
                     SSEDLKAVSLSITSKTNVNYTIEIYTDLKDKTKPTSGTKQETATTTGQTTYAGVYTIP
                     LKAGVNLKPGTSYSVVVTTDTPAIDIEAAMTGDVNYNNEMVWENHVSSDNTASYYFYG
                     TGLTYSRNWNYQNVYGNFCIKAFTANNVEKDSEKLVGRSLTLKDNIDMNYYMELPESI
                     KSNSNAYMEFTVNNSQPYKVSVNDAIPVEKNGKVIYKFACPLNAAQMSDTVKAKMVVD
                     GNSGNEYTYSVKEYAT"
     gene            complement(1994..2536)
                     /locus_tag="DHV81_02560"
     CDS             complement(1994..2536)
                     /locus_tag="DHV81_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93403.1"
                     /translation="MKFNFNKKMVSTIVILIFGVAITAAALSAKNIALENKTSSEIAT
                     EAVSATEMILDTENTETGNVDKNSSDSSKDKATVVDDKDKSGDIKNSDKSSTTTKETG
                     KSLKDTKSSSENTDNNDSRDSYETDIMKNDESENTSDDVNKTGDKIDKKSEINNSGMN
                     NNNEPKSDNGVIELPIIPID"
     gene            complement(2533..3066)
                     /locus_tag="DHV81_02565"
     CDS             complement(2533..3066)
                     /locus_tag="DHV81_02565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93404.1"
                     /translation="MKLEEIQLAKKHAYIKENYEKWNFLEAFSASDDYKTEPIDFYFE
                     FKKSSTCSLAELVKKEHRYICNDGYIKEHSIDDLSECIVVHNRKQALKIFANINRIFD
                     DYLSKAGLEGIEYYRQYFEICVRRDGMPQVFLPYSWTSKEYLIKEEPLLLLSDLQKFI
                     NQVVLIDYIIIILGYRK"
     gene            complement(3115..5178)
                     /locus_tag="DHV81_02570"
     CDS             complement(3115..5178)
                     /locus_tag="DHV81_02570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93405.1"
                     /translation="MKINKTKPIALGIAFTLALTPIHTITYADTNLDSASINVQSAQT
                     EYKSGFVFTKSSYAYRKGTDLTVCVSKSNLSTACDSYKLFYISDGSEKAIDRNSYTIS
                     VLGDAVYFTLSQNWLNQYSPRDIDITAIAYNGTAEVSKANSKLVSEDGFDIDFAAGTV
                     CVNQTENIRTNGQGELTQDTRSQAGGEKYIYVVDSVISQDESIIKISNAGNEWKYTGM
                     KPGKTKLTVRYHYYYGKEKRTGEKTAEYEVVPATYTLDEERGSTGMLINRGASYTCNL
                     RTKQITYDKSGKAVSKYVDNVAYEWYISEKEENVAVNIEQTAQNKLKITPANASESGA
                     FCIGIRALDASGNVYFEEESIWYLVTDGDHGDSSETANPGNTSSGSQNTNTGSEKVSV
                     SKSQSISLLENHNIGLNIYWKMTGSTYDIGDCMVKYTYEGDSGTPHYVALNRSPVGDG
                     ESYCSRIDISAVEMTEKVNLQLLSNSGKVLDTFSTSVEDYAKSLLASNTKEYAAYKPV
                     VKAMLNYGAASQQYFGFMTYALANRSLTNADKTIQQIPQATLKQYAANSSIRQFSMSG
                     IHYYGSSLVVGDDVKLRHYFKLDSGRDISNYSFATYVGGDMIGYATPCRSKDKDMYYI
                     EVTCTNRNFFSGMRTIVSNGNTETILDYQPMNYIAKAYGSSKLTSELKTLLDAMYWYE
                     YQKIK"
     gene            complement(5672..6484)
                     /locus_tag="DHV81_02575"
     CDS             complement(5672..6484)
                     /locus_tag="DHV81_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93406.1"
                     /translation="MAKENVNELVDKLTLFDDDLMSRVFDNNIEATELMLRIILGRNI
                     NVISVIGQDEIKSHEVGGRNITLDVHAIDVNGDEIDIEVQGNSAGAHIRRARYHSSMV
                     DSKMLDEGQEFRQLKDSYIIFIYKHDKFRKGLPVYHIDRYIRETEELFNDGSHIIYVN
                     GNYKGNDEIGQLMNDFRQTDSDKIHYAALAKGVKHFKDTEKGREQMCEAVEKYAKEYA
                     KEYAKEYAVKEKMISVKNLMENMKLTVDQALNALGIQGDERKTIINQLQNRN"
     gene            complement(6679..6867)
                     /locus_tag="DHV81_02580"
     CDS             complement(6679..6867)
                     /locus_tag="DHV81_02580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93407.1"
                     /translation="MDISPYELLSGAEHIGTRSRDNQTYVFAKDTELGMVVETYQQLD
                     YEQQKRLWGYMDALKMNN"
     gene            complement(7231..7305)
                     /locus_tag="DHV81_02585"
                     /pseudo
     CDS             complement(7231..7305)
                     /locus_tag="DHV81_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7628..8920)
                     /locus_tag="DHV81_02590"
     CDS             complement(7628..8920)
                     /locus_tag="DHV81_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCI93408.1"
                     /translation="MKLDSITLQNFKGIDNLEIKLDNKTTVIFGVNGVGKSTILQAID
                     LLYADIIAKLMGTVRSKTARFNEDFISYGKSAAGIKADFDFGDGESIYYERTIDRAKG
                     EKRNTSALKKLTDKFQSLYIQMGYDDGNGNWIEESDNKSMPIFVNYGVNRIVLDVPVR
                     APKEQFVKLDAFDKAIESTIDFRNLFKWFRNQEDIENQIKVRDDSKYEDKSLTAVKKA
                     MLAMLEGFEDIRIERRPLAMKVNKNGKSLKIDQLSDGEKCTIALFGDLARRMALANPG
                     KDVNPLEGSGVVLIDELDLHMHTSWQRKVLNVLRDTFPNIQFIITTHSPQILGEMDDS
                     VNLLYLYNEGNEILFKSYESFVGWDANVILEEVMNTSSVNQDIKRMIDEMYKCIEDKK
                     YDEAEKIVDILDKKTNGYADGISKARILISRGKRREAN"
     gene            9078..9998
                     /locus_tag="DHV81_02595"
     CDS             9078..9998
                     /locus_tag="DHV81_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase XerC"
                     /protein_id="HCI93409.1"
                     /translation="MENLQTLTENYLEYCRTQKCLDEKTLKAYRIDLRQFSEEPYLVK
                     ITEITPNNLENYIARLHQQYKPKTVKRKIASIKAFFHYLEYKELLAINPFTKTQVHFR
                     EPTILPKIIPLHTVEKFLTIIYTQRDNAKTNYQRRNALRDAAVSELLFATGMRISELC
                     SLKNEDINLHDGSILIYGKGDKERRIHIGNDSVSKIIEEYKAEFQVEIKSCNHFFANQ
                     NGKPLSDQAVRRMINKYCSLADIDLHITPHMFRHTFATSLLEADVDIRYIQEMLGHSS
                     INVTEIYTHVTTAKQRDILISKHPRKDFHI"
     gene            10113..12434
                     /locus_tag="DHV81_02600"
     CDS             10113..12434
                     /locus_tag="DHV81_02600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93410.1"
                     /translation="MKKCKLYLQISMVVISVFVGVTFSVYTFYYLYNHNFDMAGIYAT
                     LIGIIMPLIISICSYIITKIRKGTLIERYLNDEHFVDRDIEYIKLSNLIKNESDRIIY
                     IKGKFGMGKTSFMKMSCDRINYTDKNRWKSYAAFYYNNNYTKTITQALSNKFCGNPSA
                     SVTDISNKLNDSTLQRNSILFIDNIYEMNLIECTEFAKAFINCSRNNQVVIAVDSNDA
                     VFHICPGQFSENEIELLAHSYNIEINPSERHAISELSSGYPVYARYSVEAYTKGLNII
                     DYNNLEYYIEKLLNSLSDLEKSALSLIICLCQILQDGVERKTICGIDNAVTRPVIKRL
                     ITYSLINMYKEKIYIDKLISLKCMDFLAPYSNVSYNKIYHYYKGMTNTSYIALVAAFK
                     SDFQYDHNLIGNILHKQYADNNFYLLIAIGELEFDGQINPHLRENRECWIYVRYYYLK
                     ALLELGLYDRAREVVDSYDYQIRGGINLLSINSDIEFEYQYLLVDLDHLTNYLKDAVT
                     FSYALLEKASTKAQIVKCRYLYAHCLRHLGEDLEQAYTVFDDLINDADNNDDKIRIRS
                     IYSAASIRMFQCNINYPYKKAFDDIEQIICNNTANEIWKPYVARHKAIYEYKIRKNFR
                     LAEKILLETIDLLEVTPLRIKYDIYFELGEIYRLMDTELNNYEKSQKYYLEAVQFAER
                     VHDFNLYSNSQLGIMLLSIKYEKEIDNDILRTIISETYSLGLNINYNYAIYVQHLIRN
                     EDFSKELVTYWRKMHFSDLKFFYSLNTDFFIRF"
     gene            complement(12384..12521)
                     /locus_tag="DHV81_02605"
                     /pseudo
     CDS             complement(12384..12521)
                     /locus_tag="DHV81_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            complement(12544..13647)
                     /locus_tag="DHV81_02610"
     CDS             complement(12544..13647)
                     /locus_tag="DHV81_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HCI93411.1"
                     /translation="MFGQKRIPSREGGVEIVVEELCTRMVAQGHNVTCYNRGGHHVSG
                     SEYDSKRLKEYKGIKLKTVPTIEKKGLAAVSSSFFAALCCAFGQYDIVHIHAEGPAFF
                     AWLPKMFGKKVIVTIHGLDWQREKWKSGFGSKFIHQGEKNAVKFADEIIVLSKGVQDY
                     FEKTYGRKTVFIPNGVSHHIEREPQIIKNKFGLDKDKYILFLGRLVPEKGIKYLIEAF
                     KQVDTDKKLVIAGGSSDTSEFENEMKELAKEDKRILFTGFVQGQELEELYSNAYVYVL
                     PSDLEGMPLSLLEAMSYGNCCLVSDIPECVDVVEDKALIFQKSDVNSLYTKLVDICCN
                     SSKVHSFKRTAEEFVCNKYSWNKIVKDTCCLYR"
     gene            complement(13698..15146)
                     /locus_tag="DHV81_02615"
     CDS             complement(13698..15146)
                     /locus_tag="DHV81_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCI93412.1"
                     /translation="MYRKKGRGWTKHIDFMLIDIACIVIAFFISYVIRFGFNNPYMDK
                     DYRILGVAFLLIDFFVEIMADSFKNVLKRGYLDELISTCKHAMLVFLATALFLFTTQM
                     ADIYSRLSFYIMFPIYVTISYVARIALKSFLKKKGFGASKKSLLVIAPDAMLRDTLRT
                     VEKSCIGYTRIVAASLDTDMKGKTICSIPIVANHDGIVDYACDEWVDEVLIPPCSEDE
                     YPEKMADIFLEMGIAVHTGIAKNGTAQGGYKQIEKIGDYTVVTSSVNYANTSALLVKR
                     GMDIVGGLVGCLFTLIIMIFVGPMIYIASPGPIFFSQERIGRNGRKFKMYKFRSMYMD
                     AEERKKELAAQNKVGDGMMFKMDFDPRIIGNKVLPNGKKKTGIGQFIRKTSLDEFPQF
                     FNILVGDMSLVGTRPPTLDEWEKYEPHHRARMSFRPGLTGLWQVSGRSNITDFEEVVK
                     LDTQYIGEWSVKGDISILFKTVMGVLKSDGAV"
     gene            complement(15439..17034)
                     /gene="hemZ"
                     /locus_tag="DHV81_02620"
     CDS             complement(15439..17034)
                     /gene="hemZ"
                     /locus_tag="DHV81_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HCI93413.1"
                     /translation="MIVVVFNKPDFEYDVHSLLKEFFPQEDVQMYYSCPPDEVEGKNL
                     ACTHHEMTDDGVKEFADASQVFKIDYVGDEIAVEWTVNRTGGNNSMTDEMQHADKSDI
                     ETAGKSICTKISVTGADRKETKNHLKLALYSMIEKGTGKSLPWGTLSGIRPTKIAMKC
                     IEDGMSDKETYDYLKETYLASDEKIDLSIGIAKREKALLDKVDYDNGYSLYIGIPFCP
                     STCAYCSFTSYPLGVWKNRVDDYLDALEKEIDYTAQKFYHRKLNSIYIGGGTPTTLSP
                     AQLDRLIRKIKCSFDLKDCLEFTVEAGRPDSITREKLMALKKNGISRISVNPQTMKQQ
                     TLDIIGRHHTVDDTIQSFKLARELGFDNINMDLIMGLPEETLDDVRHTMELVKELAPD
                     NVTIHSLAVKRAARLTIFKDRYSDMQMINTQEHMDLCAAYCKQMGLEPYYLYRQKGMA
                     GNMENVGYAAKGKAGVYNILIMEEKQTIVACGAGASTKRVWPIPNPDGTHRIDRCENV
                     KDVGQYIARIDEMIERKQRLFEE"
     gene            complement(17031..17660)
                     /locus_tag="DHV81_02625"
     CDS             complement(17031..17660)
                     /locus_tag="DHV81_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCI93414.1"
                     /translation="MVNMKIDHFVVGPVQTNCYFVTNIKTKEMIIIDPGACANQLAKK
                     VDESGAKPVAILLTHGHFDHATDAKPLADRYGIKIYIHEADKATLDDPQLNVSYMMGQ
                     SKTFYADVFLKDKQELDIAGFHIVVLHTPGHTPGGCCYYFPYEDVIFTGDSLFCGSIG
                     RTDFKGGSMSDLVRTVKEKIMTLPEHTEVYPGHNDTTTIENERMYNPYL"
     gene            complement(17716..>19464)
                     /locus_tag="DHV81_02630"
     CDS             complement(17716..>19464)
                     /locus_tag="DHV81_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCI93415.1"
                     /translation="ARETQDIYCPIAQRLGISKIKIELEDLCMKYLYPDAYYDLVEKV
                     ALRKAERDTYIQGLVNDVKKYVTDAGIKAEIYGRAKHFFSIYKKMVNQDKTIDQIYDL
                     FAIRILVDTIPDCYAVLGIIHEKYKPIPGRFKDYIAMPKQNMYQSLHTTLIGPSGQPF
                     EIQIRTYEMHRTAEYGIAAHWKYKEANNGNATTTTVTEEEKLSWLRQILEWQQDMSDN
                     KEFMTLLKSDLNLFSDNVFAFTPSGDVKNLPKGSTPIDFAYSIHSAVGNKMVGAKVNG
                     KLVPIDYVIKNGDQIEIITSQNSKGPSRDWLKVVKSTQAKNKINQWFRSELKEENIQH
                     GKDLIAAYAKAKGINFAEINKPEYQEKIRRKYGFHDWNSCLATVGHGGLKESQIVNRM
                     YDEYKKDHVVPVTDADVLGRIAEKQQAAAGTQPEHKSKSGIVVHGLYDVAVHFSKCCN
                     PVPGDEIVGFVTRGRGISIHRTDCVNIINLSKIEKDRLIEAEWQDTALMDNGAEYQAD
                     LKIFCHDRPGLLVDITKIFTEKEINISGIQSKTSKQGIATIEVFFNIKGRDQIKVLVE
                     KLRQIESVIDVERTTG"
BASE COUNT         5363 a         4320 c         3168 g         6613 t
ORIGIN      
        1 gtggcatatt cttttacact gtaagtgtat tcatttccac tattaccatc caccaccatt
       61 ttagctttaa ccgtatctga catctgtgca gcatttagtg gacatgcaaa cttatatata
      121 acctttccgt ttttctcaac agggattgca tcgtttacgc ttaccttgta tggctggcta
      181 ttattcaccg taaactccat atatgcgttg ctgtttgatt tgatactttc aggaagctcc
      241 atatagtagt tcatgtcaat attatctttt aaagtaaggc tcctgccaac aagcttctca
      301 gaatcttttt ctacattatt ggcggtaaat gctttaatac agaaattacc atacacattc
      361 tgataattcc aattgcggga atatgtcaac cctgtgccat agaaataata gcttgccgta
      421 ttatcacttg acacatgatt ctcccacacc atttcattat tataatttac atcaccggtc
      481 atcgctgcct caatgtcaat ggcaggtgta tcagtagtca ctacaaccga atagcttgtc
      541 ccaggcttca gattaacccc tgctttaagt ggaatggtat atactccggc ataggttgtc
      601 tgccctgtag tggtagcagt ctcctgcttt gttccgctgg ttggctttgt cttatctttt
      661 aaatctgtat atatctctat tgtataatta acatttgttt tagatgtaat agacagggaa
      721 actgctttta agtcctcaga agatactccc tgttttgcct gagtagtaaa aacattggcc
      781 aattttctgt aaccgctttg atgggctaca tttaatcctc catcaacctg ataattgtta
      841 tcatatccat cattagcaga aaaatcaaac acccaagccg tatcggataa agaaaacgtt
      901 tcatatgaca tccagaaata actctgatta taatatgaac cggttccgtc tccccaacta
      961 ttacgaataa gccatgcacc atctttttca ggtttaacac catctctgaa attatcttta
     1021 gaatagttat catcccatcc aacaaccatt accgcatgac ctgctctgtt atttgtttta
     1081 tcattataac tgttatttat gtaatgatat ccgttatcat tatgactata cataattccc
     1141 actgcaccat atttttttat ggcttttttc acatctgatg cattttcctt gatattaatc
     1201 atgtatgtat tttccagatg tgccttatcc tttgaatatg catattcatc agataaacct
     1261 gtattaagaa aagtatcaat agtgcttgct gtatatccgt tgcttgccac cttactatat
     1321 ggtacatcac tctcgcttac cggaccacac cactgtccca ttctgcgtgc cgccatcaaa
     1381 tagttgccac cacgattgag ataaccatac tgaacacttg tattattcat atagtacttt
     1441 gcatagtccc catctgttcc accaagcgga tctaacagcg aattatatgc ataatatgcc
     1501 agatgcagtt cactaagatc tatattttta tcaaacgcac catcattaat cagatcaaac
     1561 tctgcaaggc ctattgacga aaatgtccag catgtgccaa gaggattttg attacgaaca
     1621 gacggatatt tcgctttgat atcgctgact ccattagccg gatacttgct ctctatagtt
     1681 tcatctgcag aatatcccca atcatcagaa tacggcaccg cctcatccgg ttcatacacc
     1741 ggtgtattat aatccaaatc actcagaata tatccacttg taatgctatt ttctacaatt
     1801 ggctgttgtt ctgcttcaac gccttgtcct gctgacaccg gttctgctgc caccggaaca
     1861 gccacaccta atatcatagc cgcacttaac accaatgata aaaacttttt tgtcttcatt
     1921 cgactttctc cattgtatta actcctctta cgtttcttct tcaaatgtag gtaaaaccac
     1981 aactgacgtc acattagtca atcggtataa ttggaagctc tatcactccg ttatcagatt
     2041 tcggttcatt attattattc attcctgaat tgtttatttc agatttttta tcaatcttat
     2101 cacctgtttt atttacatcg tctgaagtgt tttctgattc atcatttttc attatatcag
     2161 tctcatatga atccctggaa tcattattat cggtgttttc tgatgagctc tttgtatctt
     2221 tcaagctttt gcccgtttct tttgtagttg ttgaactttt atcagaattc tttatatcac
     2281 cagatttgtc cttatcatcg actactgttg ctttgtcttt tgaggaatcg gatgaattct
     2341 tatccacatt accagtctct gtattttctg tatccaatat catctctgta gctgatactg
     2401 cctctgtggc tatttctgag cttgttttat tttcaagcgc tatattcttt gcagacaatg
     2461 ctgcagctgt aattgctacg ccaaaaatca atatcactat tgtgctaacc atttttttgt
     2521 taaaattaaa tttcattttc tatatcctag tataattatg atgtagtcaa tcaagactac
     2581 ttggttaata aatttctgta aatcagataa caaaagaagg ggttcttcct ttatcagata
     2641 ctctttactt gtccaactat atggcaaaaa tacttgtggc attccatctc ttcttacaca
     2701 aatttcaaaa tactgccgat aatactcaat tccttcaagc ccagccttgc tcaaataatc
     2761 atcaaatatt ctattgatat ttgcaaatat tttcaaagcc tgcttacggt tgtgcactac
     2821 aatgcactcg cttaaatcat caattgaatg ctctttaata tacccatcat tgcatatata
     2881 tctatgttcc ttttttacca attcagccaa gctgcaagta gatgactttt taaattcgaa
     2941 atagaaatct ataggttctg tcttataatc gtcagacgct gaaaaagcct ctaaaaaatt
     3001 ccacttttca taattttctt ttatatatgc atgcttctta gcaagctgaa tttcctctaa
     3061 cttcaactct caaagtcagg aattatactc tagtactata ttttatgcaa caatttattt
     3121 aattttttga tattcatacc aatacattgc atcaagcaac gtcttcaatt cagaggttag
     3181 ctttgaactg ccatatgctt ttgcaatgta gttcatcggc tgatagtcca atatagtttc
     3241 tgtatttcca ttcgatacta tcgtcctcat gccagaaaag aaatttctgt ttgtacaagt
     3301 tacttctata taatacatgt ctttatcttt cgaacgacat ggtgtagcat agcctatcat
     3361 atctccgccc acatacgttg caaacgaata atttgatata tctctgccgg aatcaagctt
     3421 aaaatagtgt ctgagcttta catcatctcc caccacaagg ctcgatccgt aatagtgtat
     3481 tccactcatt gaaaattggc ggatacttga attggcagca tactgcttca gcgttgcctg
     3541 tggaatttgt tgtatcgtct tatcggcatt cgtaaggctt ctgttggcta atgcatatgt
     3601 cataaatcca aaatactgct gtgatgcagc gccataattc aacatcgctt ttaccaccgg
     3661 cttatatgct gcatattctt ttgtattgct tgccaataag ctcttggcat aatcctctac
     3721 tgatgttgaa aatgtatcca gcacttttcc actattagag agtaactgta aatttacttt
     3781 ttcggtcatc tcaactgcgc ttatatcaat tcttgagcaa tatgattcgc catcaccaac
     3841 cggactccgg ttcaatgcca catagtgtgg tgttccgctg tcaccttcat aggtgtactt
     3901 gaccatgcag tctcctatgt catacgtaga gccggtcatt ttccagtaga tatttagacc
     3961 gatattatga ttctccaata aggatatact ctgactcttc gatacactta ccttttcgct
     4021 tcctgtgttt gtattttgac ttccggatga ggtatttccc ggatttgcag tttctgatga
     4081 atctccatga tctccatctg ttaccagata ccatattgat tcttcttcaa aataaacatt
     4141 accggaagca tcaagtgctc ttattcctat acaaaaagct cctgattccg aggcatttgc
     4201 cggtgttatt ttaagcttat tctgtgcagt ttgttctatg tttaccgcaa cattttcttc
     4261 tttttcactt atataccatt cgtatgcaac attatctaca tatttactta cagccttgcc
     4321 actcttatca taggttatct gctttgttct aagattgcat gtatatgagg cgccgcggtt
     4381 tataagcata cctgtgctgc cacgttcttc atcgagtgta tatgtggcag gaaccacttc
     4441 atactcagca gttttctcac cggtacgttt ttccttgcca taatagtagt gatagcgtac
     4501 tgtgagtttt gtctttccag gcttcattcc ggtatatttc cactcattac cggcattgga
     4561 tatttttatg atgctttcat cctgagaaat tactgagtca actacatata tgtatttttc
     4621 cccgccagcc tgcgaccgcg tatcctgtgt cagttcgcct tgtccgttag ttctgatatt
     4681 ttctgtctga ttgacacaca ctgttccggc tgcaaagtct atatcaaagc catcctcaga
     4741 aacaagctta gaatttgcct tagatacctc agcagttcca ttgtaagcaa tcgctgtaat
     4801 atcaatatcc cgaggactgt actgattcag ccagttttgg gataaagtaa aatataccgc
     4861 atccccaagg acactgattg tatatgagtt ccggtcaatt gctttctcac ttccatccga
     4921 tatataaaac agcttatatg aatcacaagc cgtagaaaga ttgctctttg atacacatac
     4981 tgtcaaatca gtgccttttc tataagcgta gctgctctta gtgaatacaa atcctgattt
     5041 atattctgtc tgtgcagact gaacatttat tgatgcactg tctaaattag tgtctgcata
     5101 tgtaattgtg tgaatcggtg ttagggcgag agtgaatgct attcctaacg caattggttt
     5161 tgtcttatta attttcatgc tcaacttttc ctaacataat aatttctcta ccccaataat
     5221 tatataacat atacctgttc tattgcaatc ttacacgcca ctgcattagt gtatcgtatc
     5281 atcaatagcc acctcaatac ctgcatcttc gatttgatgc gtcgtataat tacaaagctg
     5341 tgcatcattc ttaattttga ccacattggt gcctgacccc actgtggtct ttcatctata
     5401 ctttgataaa gcttaataag gacagtataa tggcctatgg ctgaacaatc aagaggtcag
     5461 gtgatggaca agataagaaa tcataaacag taaacattag aggcaactag cctaaacgga
     5521 tgctctactg tttaacatat aatagaagcc ccagtagttc cagctactgg ggcttctatt
     5581 tgggtgtgcc atgacactct ttagcatttc aatctagaac atcttatatt tatgttctaa
     5641 aaatttttta atatatctct acttaaatat cctaatttcg gttttgtaat tgatttatta
     5701 tggtttttct ttcatcccct tgaattccca aagcatttag tgcctgatcc actgtaagtt
     5761 tcatattctc cataagattt ttcacagaaa tcatcttttc ctttaccgca tattcctttg
     5821 cgtattcttt tgcatattcc tttgcatatt tttcaactgc ttcacacatt tgctcacgcc
     5881 ccttttcagt atccttaaaa tgctttacac cttttgctaa tgcagcataa tgaatcttat
     5941 ctgaatctgt ctgacgaaaa tcattcatta gctgtccaat ttcatcatta cctttatagt
     6001 taccatttac atatataata tgggaaccat cattaaataa ttcttctgtt tcccttatat
     6061 atcggtctat gtgatatact ggtaaaccct ttctaaattt atcatgttta tatataaata
     6121 tgatatacga atccttaagt tgcctgaact cctgtccttc gtctaacatt ttagaatcta
     6181 ccatactgct atgatatctt gctcgtctga tatgtgcccc tgccgaattc ccttgtactt
     6241 caatatcaat ttcatcaccg ttcacatcaa tcgcatgaac atccaatgtt atatttcttc
     6301 cacctacttc atgacttttt atctcatctt gtccaataac actaattaca ttaatgttcc
     6361 ttcccaatat gattctaagc ataagctctg ttgcttcgat attattgtcg aaaactctac
     6421 tcattaaatc atcatcaaat agagtaagtt tatccaccag ttcattaaca ttttccttag
     6481 ccattatatc ctctctttca aattcatatt accacgtatc ggtatttcaa tcaaataaaa
     6541 aaatcataaa gaatgaatta ttctttttgc atctggacaa ccatacccag tactcttcat
     6601 ttgaatatat aatcttcacc acctcttgta cgaatttttt cgtttttacg tatcatgtgg
     6661 tattaaatta caatttttct agttgttcat cttcaaagca tccatatagc cccaaagtct
     6721 cttttgctgc tcataatcca actgctggta tgtctcaact accattccaa gttcagtatc
     6781 ttttgcaaaa acatatgtct gattatccct gcttctggtt ccaatatgct ctgctcctga
     6841 caataactca tatggcgaaa tatccattcg tcctaattca atgtatttac tttttcattc
     6901 gaacaaaacc tcgaacacct agcttaatgg cattgccacc accaccatat taggcggggg
     6961 agtgcacgga aaaaagcgta ggcatagcga gctacgctta ggatttttac catatccact
     7021 gattacgttc ctcatttgcc tcatcagatg atttacaaat ctcgcgccat ttttccacac
     7081 ccttaccgca atttactgtc ttccaatcat tcctgccctc ctttaaagta acaacatttc
     7141 tctgtagaac ttcgttatcc tataaaacca ctttcgcata tgctgtttta tcagccctat
     7201 ttttattatg ataaattgca cctgtcaacg gttgttctct aataagaact cagctaaata
     7261 agaaccatcc tgtcctgtaa ttcctgttat aagtactttt ttcatttgaa atctccttaa
     7321 aatagataaa aatacgtctg tttaaaatac gataatattt taaacagacg tatctaaaaa
     7381 tgtaatcgac aatatttgta taaattagca caatattggt ttttatcact accactttaa
     7441 aatcatcaaa gcatctcctc caaacaatca acaattgctt tgcaataagg ctcatattca
     7501 ccattattct tatttgaata atattctata aaactacgaa tctcatcatc cgtatagtcc
     7561 tcatcatcaa acacctccga tgcctgaata gcctgcttgt tcaaaccatt gcggcgcact
     7621 gcttttatta atttgcttca cgtcttttac cccttgatat tagtatcctt gccttagaaa
     7681 taccatcagc ataaccattt gttttcttat ccaatatatc cactattttc tctgcttcat
     7741 catacttctt atcttcaata cacttgtaca tctcatcaat cattctcttg atgtcctgat
     7801 taactgaaga cgtattcatt acttcctcta aaataacatt tgcatcccat cccacaaagg
     7861 attcatagct cttaaataga atctcattac cttcattata cagatacaat aaattgacag
     7921 aatcatccat ctcacccaaa atctgcggag aatgtgtagt aataataaac tggatattgg
     7981 ggaatgtgtc tcttaaaaca ttcaatacct ttcgttgcca cgatgtatgc atatgcaaat
     8041 ccaattcatc aatgagcaca acacctgagc cttccaaagg attcacatct ttacccggat
     8101 ttgccaatgc cattcttctt gccaaatccc caaatagagc tatcgtgcat ttttctccat
     8161 ccgataactg atctatcttt aaagacttgc cgtttttatt gaccttcatt gccaaaggtc
     8221 ttctctctat ccgaatgtcc tcaaatccct caagcatagc taacattgct tttttaacag
     8281 ctgtaaggct tttatcttca tattttgaat catctctaac ctttatctga ttctctatat
     8341 cttcctgatt tctgaaccat ttaaataaat ttctaaaatc tatcgtactt tctatagctt
     8401 tgtcaaatgc atccagtttt acaaactgct ctttaggagc cctgaccggc acatcaagca
     8461 caattctatt tactccataa ttaacaaaaa tgggcatact cttattatcc gattcctcta
     8521 tccaattgcc attgccatca tcataaccca tttgtatata taacgattga aacttatctg
     8581 taagtttttt aagagcactt gtatttcttt tttcaccttt agccctgtca attgtgcgtt
     8641 cataatatat gctttcgcca tccccaaaat cgaaatctgc ctttatgccg gccgcagact
     8701 ttccatagct aataaaatct tcattgaatc tggctgtctt acttcttacc gttcccatca
     8761 gcttcgcaat gatatcagca tataataaat ctatagcctg aaggattgtt gacttgccaa
     8821 ctccattcac accaaaaata accgttgtct tattatcaag ctttatttcc agattgtcta
     8881 ttcccttaaa attttgtaat gttatcgaat caagcttcat atccctacct cacaataact
     8941 cttcagattg cagtaaaaac cgctatacca tgcttatagt atagcggatt tgtatttaaa
     9001 aaacaattta tattttacca ctttttccga tataatttta tcaatttatt ttaaaacatc
     9061 aaacaaagga gaacatcatg gaaaacttac aaacacttac tgaaaactat ttggaatact
     9121 gcaggacaca aaaatgtctg gatgaaaaaa ctttaaaagc atatcggatt gacttgcgac
     9181 aattctctga ggaaccatat cttgtaaaaa taacggaaat caccccaaac aatctggaga
     9241 attatattgc caggctccac caacaatata aacccaaaac cgtaaaaaga aaaatagcct
     9301 caattaaagc attcttccat tatctggaat acaaagaact actcgcaata aatcccttca
     9361 ccaaaacaca ggtgcacttt cgtgaaccta ccatattacc caaaattatc ccactacata
     9421 cagtagaaaa atttctgact atcatttata cacagcggga caacgctaaa acaaattatc
     9481 agaggaggaa tgcattgcgc gatgcagccg tatccgaact gttatttgct accggcatga
     9541 gaatttctga gctttgctca ttaaaaaacg aagatataaa tttacatgat gggagcatcc
     9601 tcatatatgg taaaggcgat aaagagcgac gtattcatat tggcaatgat tctgtttcta
     9661 aaataattga agaatataag gctgaattcc aagttgaaat taaaagctgc aatcattttt
     9721 ttgctaacca aaatggcaaa cctctatctg accaagctgt tcgcaggatg ataaataaat
     9781 attgctctct cgccgatata gatttacata tcactcctca catgttcagg catacattcg
     9841 ctaccagctt attagaggcc gatgtcgata tccggtatat tcaggaaatg ctaggacata
     9901 gctccatcaa tgtcactgag atatacacac atgtaaccac tgctaaacag cgggatattc
     9961 taatatcaaa acatcctagg aaggattttc atatatagac ttctttacaa tccatcgttt
    10021 ttgatggatt tttttatttt taagggataa tttttagtta tccgttgatt atctactaat
    10081 cgttttatga ataatatcaa agaggacttt gtatgaaaaa atgcaaatta tatttacaaa
    10141 taagcatggt tgtcatatca gtatttgttg gtgtaacatt ttctgtatat acattttatt
    10201 atttatataa tcataacttc gatatggcag gtatttatgc cacactaatt ggtataatca
    10261 tgccattaat tatctcaatt tgctcctata taatcaccaa aatacgaaaa ggcacattaa
    10321 tagagcgata cttaaatgat gagcattttg tggatagaga tattgaatat ataaaattgt
    10381 ccaatttaat aaaaaatgag tccgatagga taatatatat caaaggcaaa tttggtatgg
    10441 gaaaaacgtc gttcatgaaa atgtcttgtg atcgaattaa ttacactgat aagaatagat
    10501 ggaaatcata tgctgcattt tattataaca acaattatac caaaacaatt actcaagctc
    10561 tttcaaataa gttctgtgga aatccaagtg catctgtaac ggatataagt aataagctga
    10621 atgattctac tttgcaaaga aatagcatat tatttatcga taatatttat gaaatgaatt
    10681 tgatagaatg tacagaattt gcaaaagcat tcataaactg tagcagaaat aatcaagtag
    10741 tgattgcagt cgatagtaac gatgcagttt ttcatatatg tccgggacaa tttagtgaaa
    10801 atgaaattga gttacttgca catagctata acatcgagat taatccatct gaacgacatg
    10861 caatttccga actatcgagc gggtatccgg tgtatgctcg ttacagcgtt gaagcatata
    10921 ctaaagggct taatattata gattataata atcttgagta ttatattgaa aaattgctca
    10981 attcacttag cgaccttgaa aaaagcgccc tatcattgat aatttgcctt tgccagatat
    11041 tgcaagacgg agttgaaaga aaaactatat gcggaatcga taatgccgta acacgaccag
    11101 tcattaaacg attgattacg tattcattaa ttaatatgta caaagaaaaa atatatatag
    11161 ataaattgat ttcgcttaaa tgtatggatt ttttagcacc atacagcaat gtaagttata
    11221 acaaaatcta tcactattat aagggtatga ctaatacttc ttacattgca ttggtcgcag
    11281 ccttcaaatc agatttccaa tatgatcata atttgatagg caacattctg cacaaacagt
    11341 atgcggacaa taacttttat ttgctaatag ctattggtga gttggaattt gatgggcaaa
    11401 taaacccaca tttacgtgaa aatagagagt gttggatata tgttaggtat tattatttga
    11461 aggcacttct tgaactggga ttatatgata gagcaagaga agtcgttgat agttatgatt
    11521 atcaaattcg aggtggaatc aacctattaa gcataaatag tgatatagaa tttgaatatc
    11581 agtatttatt agttgatttg gatcatctca caaattattt aaaagatgca gttacctttt
    11641 catatgcttt actagaaaaa gcttcgacga aagcacaaat agtgaaatgt cgatacctat
    11701 atgcccattg cttgagacat cttggggaag atttagaaca ggcgtatacc gtttttgatg
    11761 atttgataaa tgatgccgac aataatgatg ataaaattag gatacgttct atttactctg
    11821 ctgcatctat caggatgttt caatgcaata ttaactatcc atataaaaaa gcgtttgatg
    11881 acatagaaca aattatttgc aataatacag cgaatgaaat ctggaaaccg tatgttgcta
    11941 ggcacaaagc aatttacgaa tataagatac gaaaaaactt taggctggca gaaaaaattt
    12001 tactagaaac aatagattta ctagaagtaa cgcctttgcg tatcaaatat gatatatatt
    12061 ttgaattagg tgaaatctac agacttatgg atacagaatt aaataactat gaaaaaagtc
    12121 aaaaatatta tttggaggca gtgcaatttg cggaacgagt acacgatttt aatttatata
    12181 gtaattcaca attaggaatc atgcttttaa gtataaagta tgaaaaagaa attgataatg
    12241 acatactgag aacaattatt tcagaaacat acagtctagg gttaaacatt aactacaatt
    12301 atgccatata tgtacaacac cttatacgca atgaagattt ttcaaaagag cttgtaactt
    12361 actggagaaa aatgcatttc tcagatttga agttttttta tagtctcaat actgatttct
    12421 tcatccggtt ttgaaggtgc tttataccta ttgcacatgc tacagcgagc attacatcta
    12481 taagttacta ttactgttcc gtttaatttc ttttctgaca ttttatttcc aaccttttta
    12541 tttttatctg tataagcagc aagtatcttt tacaatttta ttccagctat atttattaca
    12601 tacaaattcc tctgctgtac gtttaaatga atgcaccttt gaagagttac aacatatatc
    12661 aacgagcttt gtatataagc tatttacatc tgatttttga aatataagtg ctttatcttc
    12721 aaccacatca acacattccg gtatatccga taccaggcaa caatttccgt agctcattgc
    12781 ctccaacaaa cttaaaggca ttccctctaa gtcagatggg agtacataca catacgcgtt
    12841 gctatacaac tcttctaact cttgaccttg aacaaatcca gtaaataaga ttcgtttatc
    12901 ttctttagca agttctttca tttcattttc aaattcactt gtatccgaac tgccaccagc
    12961 aataaccaat tttttatctg tatcaacctg tttaaatgct tctattaaat acttgattcc
    13021 tttttccgga actaatcgtc caaggaacaa aatatattta tctttatcta atccaaattt
    13081 attttttata atctgtggtt ctctttctat atgatgactt acaccatttg gaataaatac
    13141 agtttttcta ccatatgttt tttcaaaata atcctgaaca cctttactaa gtacaataat
    13201 ttcatccgca aacttcacag cattcttttc accctgatga ataaattttg aaccaaatcc
    13261 acttttccat ttttcacgtt gccaatccaa cccatgtata gtgacaatca cttttttgcc
    13321 aaacattttt ggcagccaag caaaaaatgc cggtccctct gcatgaatat gaacaatgtc
    13381 atactgacca aaagcacagc ataaagctgc aaaaaatgat gacgaaacag ccgctagacc
    13441 tttcttttct attgttggaa ctgtcttaag ctttattcct ttatattctt taagccgttt
    13501 tgaatcatac tctgaaccgc ttacatgatg cccacctctg ttgtagcaag tcacattatg
    13561 cccttgtgca accatccttg tgcaaagttc ctcaacaaca atctcaactc caccttctct
    13621 tgacggaatc cttttttgcc caaacatagc aatattaagt ttttctttat gcttactctt
    13681 tcgtctattc acgtctctta aactgctcca tcactcttta atacacccat aactgtctta
    13741 aagagaatac ttatatcccc cttaacactc cactccccaa tatactgtgt atccagcttc
    13801 accacttcct caaagtccgt gatattgctc cttccactca cctgccacag tccggtcaat
    13861 cccggcctga atgacattct ggctctatgg tgtggctcat acttctccca ctcatcgagt
    13921 gttggtggcc tggtgcctac caggctcata tctcccacta gtatattgaa gaattgtgga
    13981 aattcatcca ggcttgtttt cctgatgaac tgcccaattc cggttttctt ttttccgttt
    14041 ggcagcacct tattgcctat tattctcggg tcaaagtcca tcttgaacat cattccgtcg
    14101 cccactttat tctgcgccgc aagctccttc tttcgctcct cggcatccat atacatgctg
    14161 cggaatttgt acatcttgaa cttcctgccg tttctgccga ttctctcctg tgagaagaat
    14221 atcggccccg gcgatgctat gtatatcata ggacccacaa atatcattat gataagtgtg
    14281 aacaggcagc ccaccagtcc tccaactata tccatgcccc tctttaccag aagtgcactg
    14341 gtattggcat agttcacgct tgatgtgact actgtgtagt cgcctatctt ttctatctgc
    14401 ttataaccgc cctgggctgt gccgtttttg gctattcctg tatgtactgc tattcccatc
    14461 tccaggaata tatctgccat cttctccgga tattcatcct cgctgcacgg cggtatgagc
    14521 acctcatcca cccactcatc acacgcatag tccactatgc catcgtggtt tgcaactatc
    14581 ggtatgctgc atatggtctt gcccttcata tctgtatcca gcgaagctgc tactatcctt
    14641 gtatagccta tacagctttt ctccactgtg cgcagtgtat cacgaagcat ggcatccggt
    14701 gctatgacaa gcagtgattt cttagatgct ccgaagccct ttttcttaag gaagctcttg
    14761 agtgcaattc ttgctacata tgatattgtc acatagattg gaaacatgat ataaaatgat
    14821 aatcgcgagt atatatcagc catctgtgtg gtgaacagaa atagtgctgt cgccagaaat
    14881 acaagcattg cgtgcttgca ggtcgatata agctcatcca gatatcctct ctttaggaca
    14941 ttcttgaatg agtctgccat tatctccaca aagaaatcta tcagaagaaa tgctaccccc
    15001 agtatacggt agtccttgtc catgtatggg ttattaaagc cgaagcgtat cacatatgaa
    15061 ataaaaaatg ctataacaat gcatgcgata tctataagca taaagtctat atgctttgtc
    15121 catcctctgc ctttctttct gtacattctt tccaaattct ccttatggta tcttttattt
    15181 tcctttatac ctgttctagg ggaggtacct ttgtatcccc cccccgaatt attttagcct
    15241 attttgtttt acgtatgttt tattcggtta tttgtatctt tttctgtatc taatcagggt
    15301 tttcaataat atattttcgc tcatttttta tactttgtca attaatctca tggtttcttt
    15361 acaattttcg acgtattcac taatcgctat gatattacag caccaataat tatacattat
    15421 tatatgtcat gctgcttatt actcctcaaa caacctctgc ttcctctcta tcatctcatc
    15481 aatccttgca atatactgcc ctacatcctt gacattctcg catctgtcga tgcggtgtgt
    15541 gccgtccgga ttcggtatag gccagacgcg cttagtgctt gctccggctc cgcacgccac
    15601 gatggtctgc ttttcctcca tgatcagtat gttgtatacg ccggccttgc cctttgccgc
    15661 atagcccacg ttctccatat tgcctgccat gcccttctgc ctgtagaggt agtatggctc
    15721 aagccccatc tgcttacagt aggctgcgca gagatccata tgctcctgag tgttgatcat
    15781 ctgcatatcg ctgtagcggt ccttgaagat ggtgagtctt gcagctcgct ttactgcgag
    15841 tgagtggatg gtgacgttgt caggcgccag ttccttcacc agctccattg tgtgccttac
    15901 atcatcgagt gtctcctcgg gaagtcccat gatgaggtcc atgtttatgt tgtcaaagcc
    15961 cagctcacgc gcgagcttga agctttgtat cgtatcgtct actgtgtggt gtcttcctat
    16021 tatatcaagt gtctgctgct tcatggtctg tggatttact gatatacggc tgatgccgtt
    16081 tttctttagt gccataagct tttctctcgt gatggagtct ggtctgcctg cctccacggt
    16141 aaattccagg cagtccttta aatcaaagct gcatttgatt tttctgataa gcctgtcaag
    16201 ctgcgcaggg cttagagtgg tcggtgtgcc gcctcctatg tagatggagt ttagcttcct
    16261 gtggtagaat ttctgtgctg tgtagtctat ctccttctcg agtgcgtcta ggtaatcgtc
    16321 cacgcggttt ttccacacac cgagcgggta tgaggtaaac gagcagtatg cacaggtact
    16381 cgggcagaag ggtatgccta tgtacaggct gtagccgttg tcatagtcca ccttatcgag
    16441 cagtgccttc tcacgctttg ctatgcctat tgagaggtcg attttctcat cacttgcgag
    16501 ataggtttcc ttcagatagt cgtaggtttc cttatcgctc atgccgtcct ctatacattt
    16561 catggctatt ttggtcggtc tgatacctga gagtgtgccc cacggaaggc tctttcctgt
    16621 acccttttct atcatggaat acagggcgag cttgaggtgg tttttggtct ccttgcggtc
    16681 tgcgccggtc acagatattt ttgtgcatat actttttccg gctgtttcaa tgtcactttt
    16741 gtcagcatgc tgcatctcat ctgtcatgga attatttccg ccggtcctat ttactgtcca
    16801 ctccactgca atttcatcgc ccacatagtc tatcttaaat acctgtgagg catccgcaaa
    16861 ttcctttacg ccatcatctg tcatctcgtg gtgtgtgcat gccaggtttt ttccctccac
    16921 ctcatcaggt gggcagctat agtacatctg cacatcctcc tgagggaaga actccttgag
    16981 caatgagtgc acatcatatt caaaatccgg tttattaaat actactacta tcataagtaa
    17041 gggttataca tcctctcgtt ttctatggtt gttgtgtcgt tgtggcccgg gtatacctct
    17101 gtatgctcag gcagtgtcat gatcttttcc ttcactgtgc gcacgaggtc tgacatgctg
    17161 ccgcccttga aatccgtacg tccgattgag ccgcaaaaca gtgagtctcc tgtaaagatc
    17221 acgtcctcat acgggaaata atagcagcag ccgccaggtg tatgccccgg tgtgtgaagc
    17281 actactatgt gaaagcctgc gatgtcaagc tcctgcttat ccttcaaaaa tacatctgca
    17341 taaaatgttt ttgactgccc catcatgtag ctgacattaa gctgcgggtc atcgagtgtg
    17401 gctttgtctg cctcatggat atatatcttg atgccgtagc ggtctgcgag cggctttgca
    17461 tcagtggcat ggtcaaagtg tccgtgtgtg agcagtattg ctactggctt tgcgcctgat
    17521 tcatccactt ttttggcgag ctgattagca cacgcgccag ggtctattat tatcatttcc
    17581 tttgtcttta tattggtgac aaaatagcag ttggtctgca ccggtcccac tacgaagtgg
    17641 tctatcttca tatttaccat atgagtgtac tccttgtttg ttatatgtta tgcttcacgc
    17701 aagaaccacc gggatttagc cggtggtcct ctctacgtct attacacttt ctatctgtct
    17761 gagcttctcc acgagaacct ttatctggtc cctgcctttg atattaaaaa atacctcgat
    17821 tgtagcgatg ccctgcttgc tggtcttgga ctggatacca ctgatattga tttccttctc
    17881 ggtaaatatc tttgtgatat ctacgagcag tcccggcctg tcatgacaga atatctttag
    17941 gtctgcctga tactcggcac cgttgtccat gagggctgta tcctgccact ctgcttctat
    18001 gagcctgtcc ttttctatct tggacagatt gatgatattc acacagtctg tcctgtggat
    18061 ggagatgcct cggcctcttg tgacaaaacc cacgatttca tctcccggaa caggattgca
    18121 gcacttcgag aagtgcacag ccacgtcata gagtccgtgt accacaatac cgctctttga
    18181 cttgtgctcg ggctgtgtgc ctgctgccgc ctgctgcttt tctgcaattc tgccaagcac
    18241 atctgcatct gtcaccggca ccacatgatc cttcttgtac tcgtcgtaca tcctgttgac
    18301 tatctggctc tccttgagtc cgccgtgacc gacggttgca aggcatgagt tccagtcgtg
    18361 gaagccgtat ttgcggcgta ttttctcctg atactcaggc ttatttatct ctgcaaagtt
    18421 gatacccttt gccttggcat aggctgctat cagatccttg ccgtgctgta tattctcctc
    18481 cttgagctca cttctgaacc actggttgat cttgttcttg gcctgtgtgc tctttacgac
    18541 cttgagccag tcacggcttg gtcccttgga gttctgtgag gtgattatct ctatctggtc
    18601 accgtttttg atgacatagt ctatcggcac tagcttaccg ttcaccttgg ctccaaccat
    18661 cttgttgccg accgcagagt gtatgctgta ggcaaagtct ataggtgtgg agccctttgg
    18721 caggttcttt acatcgccgg acggtgtgaa tgcaaataca ttgtcggaga ataaatttaa
    18781 atcactcttt aataaagtca taaactcctt attatccgac atgtcctgct gccactcgag
    18841 tatctggcgc agccagctga gcttctcctc ctctgtgaca gttgtggtag tggcattgcc
    18901 gttgtttgct tccttgtact tccagtgcgc tgcgataccg tactcagctg tacgatgcat
    18961 ctcataggta cgtatctgta tctcaaacgg ctgtccgctc gggcctatga gtgtcgtgtg
    19021 aagcgactgg tacatgttct gcttcggcat ggcaatatag tccttgaaac ggcccggtat
    19081 aggcttatac ttctcatgta tgataccaag cactgcatag cagtccggta tggtgtccac
    19141 cagaatcctg atggcaaaaa ggtcatatat ctggtctatc gtcttgtcct gattgaccat
    19201 cttcttatat atactgaaga aatgctttgc cctgccgtat atctcagcct tgatgccggc
    19261 atctgtaaca tacttcttga catcgtttac caggccctgg atgtaggtgt cgcgctctgc
    19321 ctttctgagg gctaccttct ctaccagatc gtagtaggca tccggataga ggtacttcat
    19381 acaaaggtcc tcgagctcta tctttatctt gctgatacca agtcgctgcg cgataggaca
    19441 atagatatcc tgtgtctctc tggc
//
LOCUS       DPUF01000072           13339 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_2423, whole
            genome shotgun sequence.
ACCESSION   DPUF01000072 DPUF01000000
VERSION     DPUF01000072.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13339)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_2423"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            32..1342
                     /locus_tag="DHV81_02635"
     CDS             32..1342
                     /locus_tag="DHV81_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI93416.1"
                     /translation="MVNGPLARKILIYSIPLMFSNLLQVFFNMTDVAVVGKFAGARAL
                     GSVGSTTIIITLTTGILLGMGGGVNAVTALHMGAEDYQRVHKTVHTSVILCFIAGLLL
                     LVAGLAFSKPLLEVMNTKPELIDGATAYLMIYLCGSPALALYNFGNGVLSAVGDTKRP
                     LIYLSISGIINIVLNLFFVIVCRLGVIGVAIASIIAQYISAALIIRFLMKTYGSYGLR
                     MKDIGIDRDAAAAVLRIGVPAAIQYSLFAIANICIQTSINSFSHVVVEGNSAATNADS
                     LIYDMMAAFYTACTSFIAQNLGARKKERVLRTFFITLAYSFLMGLVLGVALFIFQRPF
                     LLLFTSDDAVIHYGQIRIGVMAFVYCVSAFMDNATAAARGIGKSVAPTIIVVMGSVVF
                     RIIWLLTVFAYFKTLQSLYLIYVISWTLTAIAGNIYFAYHYRRI"
     gene            1481..1677
                     /locus_tag="DHV81_02640"
                     /pseudo
     CDS             1481..1677
                     /locus_tag="DHV81_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1793..2740
                     /locus_tag="DHV81_02645"
     CDS             1793..2740
                     /locus_tag="DHV81_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93417.1"
                     /translation="MSGETKEVFGSNYGQNDVIINGLIDKMTLFDDNLMSLVFGQNIE
                     TTELLLRVIMERDIKVIDVRGQDELKNPVIGGRCITLDVHAIDVDGRHIDIEVQINAE
                     GSHVKRARYHSSMMDARMLEEGQEFKEIKDSYVIFIYDHDKFRKGLPFYHIQRRVDET
                     GEAFGDGSHIIYVNGRYEGNDDIGRMMRDFHQCRPELIKSETLSKAVAYYKEKEGRGA
                     MSEAVRKYAMEYAKEYAKEYAKEYGEEQRREGMKAGIKTGIETGIETGIQTGRRTEIF
                     LSVQDGDYSVNRGAEKLGMSVDEFEKSMSEAGYRVPELV"
     gene            2779..3171
                     /locus_tag="DHV81_02650"
     CDS             2779..3171
                     /locus_tag="DHV81_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93418.1"
                     /translation="MENSNEKEDMRALIMVFVIWLLLWCVDNRQYFFEYLPHHSDYEI
                     VAAYKAFNGDMRDNIIRVYVPDRDNAIGYIKADYTDKEEKPIYVGYSKERSPHVVRKT
                     PALSKGMILAGGIIPAAIVQYFFRKKHK"
     gene            complement(3177..3800)
                     /locus_tag="DHV81_02655"
     CDS             complement(3177..3800)
                     /locus_tag="DHV81_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI93419.1"
                     /translation="MPHSTRPNSEKYEKILEALRTLLETKKISQISVSEIAQTAGIGK
                     GSIYYYFSSKEAIFDALMAKSYEEPLATAKELAKRTDISPFVRMAMIFQACRNSSAAF
                     LKIQSDAGNGVQENAFLHNKYINYLITELKPVTGEIIRQGAAEGLIVCQQPDALAEIV
                     LLVLVVKLDNTLIPSTKEETEQTISELISLLEKGTDNPEGSLNFLRV"
     gene            4032..4364
                     /locus_tag="DHV81_02660"
     CDS             4032..4364
                     /locus_tag="DHV81_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP-2 sterol transfer family protein"
                     /protein_id="HCI93420.1"
                     /translation="MTYADMFSKVKGLFMESDVSDISEHLAFQFNITGEAEGIFYAEV
                     KDGVLAVEPYEYFDRDAIFICSAETLFKLAEGRIDPILAFTTGKLKVEGNIDKALRLK
                     QIIDSKKA"
     gene            4368..5321
                     /locus_tag="DHV81_02665"
     CDS             4368..5321
                     /locus_tag="DHV81_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCI93421.1"
                     /translation="MRMKWGIIAGVLGGIAAAEAGGSAYFYRRTMMRYNAKKERTMKM
                     SGVDWESYYSFMKPHGEWMRAQTHEDVWIKSDDGLRLHATYFPGIDGGNPDKAVICFH
                     GYTSEAMSDYSSISNYYLKKGYSMLLVDARAHGQSEGKFIGFGCKDRYDALKWIDWMI
                     KKAGNGIRIVLMGNSMGGATVLMASGLNLPEQVKGIVSDCAFTSPKAVFTHVLHSMYH
                     LPAFPMIQIADFVNRKMAGYGLDECNAAKEVQKAKLPILFIHGDKDTFVPCSMCDELY
                     ASCASQKTKLIVKGAGHCESYYKNTKAFEDALDKFLEGVMR"
     gene            5321..6736
                     /locus_tag="DHV81_02670"
     CDS             5321..6736
                     /locus_tag="DHV81_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide synthetase"
                     /protein_id="HCI93422.1"
                     /translation="MKTRKGHNVYPITVAQKFHLYYAKYCPNMAVLNIGTSLTIGTEL
                     DWNVLRDSINYAYARNEAMRIRFTRDKDGECYQYIADVDEDFKERTVDFKDFTDVTME
                     EAENEMQGWTQVPFEFEDSPMTKIVMIKMPDGFNGVYFLGHHMVVDAQSLIAFLKDII
                     EIYCNAMYEGVPFPKDMCSYIEQLKKDLAYEAGSKAQLRDREFFEKLIRQSEPIYNGI
                     DGTAKLDAARELMHDNKLRSAFNASDDVTSALDIFHLEAEPTKRLMDFCEKYHISLAC
                     LLLMGIRTFFQKENGFDDVSVNNAIARRATLKEKKSGGTRIHSFPFRTCFSKDVRFID
                     AVYTIRDKQNELFRHANYNPTEYFALRSKTYPQPKAGLTYEPMSLTYQPMTLKEKGLN
                     DLGDIKYKTKWYPNGMTTQAMYLTVMHRPEDNGLDFSFEHQVKAVSRKQLEYMYYYLC
                     KIMFKGAENPELTIGEIIKLV"
     gene            6773..7003
                     /locus_tag="DHV81_02675"
     CDS             6773..7003
                     /locus_tag="DHV81_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCI93423.1"
                     /translation="MFDELVEIICNYVDVQPADVHEESRFMEDLGFTSFDFMSMLGEI
                     EDTFDVEIEQTKAAEIRTVQEAVDYLETLKDA"
     gene            7025..8539
                     /locus_tag="DHV81_02680"
     CDS             7025..8539
                     /locus_tag="DHV81_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HCI93424.1"
                     /translation="MLFHTIPDILSYANEAYGADDAIRWKKSKNEIESRTYSELKNDT
                     DSFANAIEKLGKKGQHIAVIGPSSYEWIVSYLAITESGSVAVPIDASLPAADICELLD
                     RASVRMLIFDEARSDVAEAAAKSCHDINVYVSMNSTEHCPQVLSFKGLIDDNRGSYEP
                     AVAEDALCTIMFTSGTTGKSKGVMLTQNNLAENATCLDMKIGPHTVILSVLPIHHAYC
                     LSMDILKGISLGSVICINDSIMRMAKNIQLFTPDMILMVPLMIETFARKLEEVRAAGL
                     PAEPVRKKMFGERLHTICSGGAYLNPDYVDLFAEFGITILQGYGMTECSPVISTNLSW
                     DIRKNSVGKLMPNCEAKTVDGELFVRGTSVMQGYYKMPKETEETLSDGWLHTGDLGYV
                     DEDGYIYLTGRRKNLIITKNGENVSPEELENALSVNHLIKEIIVRESEGVIEAEIFPD
                     REYAQNTGIADIRPALQALIDEYNVNVPAYKRIYSLKVRESEFEKTASRKIKRS"
     gene            8877..9888
                     /locus_tag="DHV81_02685"
                     /pseudo
     CDS             8877..9888
                     /locus_tag="DHV81_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568930.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     assembly_gap    9435..9474
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9914..10195
                     /locus_tag="DHV81_02690"
     CDS             9914..10195
                     /locus_tag="DHV81_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCI93425.1"
                     /translation="MDEVYKQIVNIAKKYNTKKVVLFGSRARGTNHPKSDIDLAIYGC
                     EDFGDLSDCLNEELWSLLKLDIINMDDKHISPVLVTEIERDGRVLYEKI"
     gene            10182..10592
                     /locus_tag="DHV81_02695"
     CDS             10182..10592
                     /locus_tag="DHV81_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCI93426.1"
                     /translation="MKKFDQYVSHLRILSRAFDEDLTNDFIVSGIIDKYYIQFELGWK
                     VLKELLRYEGANQAATGSPREIIKTAYAYYDFIDESVWLGMLRDRNDTTHIYNEEAAR
                     QLVNKVLDSYIHEFQVLEMKIKERYSDEVLSSIN"
     gene            complement(10649..11569)
                     /locus_tag="DHV81_02700"
     CDS             complement(10649..11569)
                     /locus_tag="DHV81_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HCI93427.1"
                     /translation="MMTTRELQDEIIRLKKENDICILAHAYQSHEILEVADFTGDSYG
                     LSKQAATAPQKNVLMCGVRFMAETCKILSPDKHVFLSNSAAGCPMAEQLDVELLQSLK
                     AENPDYTVVAYINTTSELKTICDVCVTSSSAVKIVKKIDNNKILFIPDCNLGAWVEEQ
                     VPEKTFKFVHGGCPTHLRMSIADVERAKAAHPAAKLLVHPECLAEVSKAADYIGSTTG
                     IIDYAKNSTDREFIIGTENSILQHLQYECPEKSFYPLSKDCVCHNMKLTTLMDVYNCV
                     KGAGGEEIILPDDIRTKAKGCIDTMLTLGE"
     gene            11850..13190
                     /locus_tag="DHV81_02705"
     CDS             11850..13190
                     /locus_tag="DHV81_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCI93428.1"
                     /translation="MNTDVLIVGSGCSGLYCALKLPRDIRITLITKSDLESNDSFLAQ
                     GGMCMLKEQSDFDSFFEDTLKAGHYENDKESVGIMINSSPDVVKDLVSYGADFARNDD
                     GSLAYTREGAHSHNRIIFHEDVTGKEITSTLLAQVKKLSNVTLMEYTTMVDIIERDNK
                     CYGAIIRKQDGMLEKVTAAYTVMACGGVGGLYRFSTNFRHLTGDSLAIAKKHGIELKN
                     PDYVQIHPTTFYTKKHEDRSFLISESVRGEGAKLLDKNMNRFVDELLPRDVLTGKIRE
                     QMKKDGTDFVWEDLRTIPRDELVSHFPNIIEHCKEMGYDVFKEPIPVVPAQHYFMGGV
                     KVNHHSRTSMECLYAVGETACNGVHGRNRLASNSLLESLVFAKRAAGQMAELGFVNDE
                     IAAKKAADSIDLADYSDEKAVEREYRKLAMEAIEGRTSVDAEETVESGIAYIQA"
BASE COUNT         3983 a         2381 c         3359 g         3576 t
ORIGIN      
        1 gtcaagagca acacagacca aagaactgga tatggtgaac ggaccactcg caaggaagat
       61 actgatttac tccataccgc tcatgttctc aaacctgctt caggtatttt tcaatatgac
      121 ggatgtggca gtggtgggaa aatttgcagg tgcaagagca ctcggctcgg tcggttccac
      181 gacaatcatc atcacactca caacaggcat attgcttggc atgggaggcg gtgtcaatgc
      241 tgtcacggca ctgcacatgg gagcagagga ctatcagagg gtgcataaga ccgtccacac
      301 atcggtcatt ctgtgcttta tagcgggact tttactgtta gtggcaggcc tggctttttc
      361 aaagccactg cttgaagtga tgaatacaaa gcctgagctt atagacggtg ctacagcata
      421 tctgatgata tatctgtgcg gttcgccggc gcttgcgctt tataatttcg gcaacggtgt
      481 gctgagcgca gtgggagata caaagagacc tctcatatac ctgagtatct caggaattat
      541 caatatagtg ctcaacctgt tctttgtaat agtatgcaga ctgggagtga taggagtagc
      601 catcgcaagc atcatagcac agtacatctc agcggcactc attatcaggt ttctcatgaa
      661 gacatacggc agctacggcc tgcgcatgaa ggatataggg attgacaggg atgcggccgc
      721 agcagtgctt cgtatcggag tgccggctgc gatacagtac tcgctctttg ccatagccaa
      781 tatatgtatc cagacatcca tcaactcctt cagccacgtg gtcgtggagg gcaattcagc
      841 cgccaccaat gcggattcgc tcatctatga catgatggca gcattttaca ctgcatgtac
      901 cagcttcata gcgcagaatc tgggcgcgag gaaaaaggaa agggtgctta ggacattttt
      961 catcaccctg gcatactcgt ttttgatggg actggtgctt ggagtggcat tgtttatatt
     1021 ccaaagaccg ttccttttgc tctttacgag tgatgatgcc gtgatacact atggacagat
     1081 cagaatcggt gtgatggcat tcgtctactg tgtatcagcc ttcatggaca atgccacagc
     1141 cgccgccagg ggcatcggaa aaagtgtagc acctaccatc atagtggtta tgggctctgt
     1201 cgtattcagg attatatggc tgctcacggt gtttgcgtat tttaagacac tgcagtcctt
     1261 atatctcata tacgtcattt cgtggacgct caccgcaatc gccggcaata tatactttgc
     1321 gtaccactat aggagaattt agcggacagc tacaataaaa ctgaatagta agcgataaaa
     1381 aacagtcaga catatgtgag agatgtgccc ggctgttttt gcggtttcgc ggctgtctct
     1441 cttttataat aggataatag gaatttgcct gatattttta ttgccctttt gcggttttaa
     1501 tgctacaatc aagccaaagc attatatttc tgttgagcga caccacagta cacattttta
     1561 tcggtgcgta tcactttgaa gagggtagaa ccacttagta tttttcgaca aagacatgga
     1621 aattttcaat gctttaagtt acctatcaat agcagggaaa atggatatgt cgattgagaa
     1681 acattatgtg aatgaagctg actggggttg ttgatgcttt atattgacaa gaaattttgt
     1741 atcatgcgat attctattct tcctgcttac acaactaaac aggaggagaa taatgtcggg
     1801 agagacaaaa gaggtttttg gcagtaatta tggccagaac gatgtaatca tcaacggatt
     1861 gattgacaaa atgacgctgt ttgatgacaa tctgatgagt cttgtattcg ggcagaatat
     1921 cgagacaacg gagttactgc ttcgcgtaat aatggagcgt gatattaagg ttatagacgt
     1981 ccgggggcag gatgaattaa aaaatccagt gattggtggg agatgtatta ccttggatgt
     2041 gcatgcaata gatgtcgatg gcagacatat tgatatcgag gtgcagatta atgctgaggg
     2101 atcgcatgtc aaaagagcca gatatcacag cagtatgatg gatgcgagga tgctggagga
     2161 aggacaggag tttaaggaga ttaaggattc atacgtgatt tttatttatg atcacgataa
     2221 gttcagaaaa ggactgccat tttatcacat tcaaaggcgt gttgatgaaa cgggtgaagc
     2281 ctttggagat ggttcgcata ttatctatgt aaatggaagg tatgaaggta atgatgatat
     2341 aggtcggatg atgagagatt ttcatcagtg caggccggag ctgataaaaa gtgagacatt
     2401 gtcaaaagcg gtggcttatt acaaagaaaa ggaaggacgt ggtgctatga gtgaagctgt
     2461 aagaaagtac gcaatggaat atgcaaaaga atatgcaaaa gagtatgcaa aagagtatgg
     2521 tgaggaacaa agaagagaag gtatgaaggc tggaattaag actggcattg agactggaat
     2581 tgagactggc attcagaccg gacgtcgtac agaaatattc ttatcggttc aggatgggga
     2641 ctattcggtg aacaggggag ctgagaagct tggaatgtca gtggatgagt ttgagaaatc
     2701 aatgagtgag gcaggctaca gagtgccgga gttagtgtaa cacgtatata tacaattaaa
     2761 atcgaggaat gtttaattat ggaaaattca aacgaaaaag aagacatgag agcacttatt
     2821 atggtatttg taatatggct tttgttatgg tgtgttgata atagacagta tttttttgag
     2881 tatctgccgc atcactcaga ttatgagata gtggcagcat ataaagcttt taatggtgat
     2941 atgcgtgata atataatcag ggtatatgta ccggataggg ataatgctat cggatatata
     3001 aaggcagact acacagacaa agaggaaaag cctatatatg taggatacag taaggaaagg
     3061 tcacctcacg ttgtacgtaa aacaccggcg ctttcaaaag gaatgatttt agcgggagga
     3121 atcattccgg cagcaatagt ccaatatttt tttagaaaaa agcataaata gtgtccttag
     3181 acacgcagga aatttagaga cccttcagga ttgtcagtgc ccttctcaag cagcgagatg
     3241 agctctgaga tagtctgttc ggtctcttct tttgtggacg gaatcagtgt attgtcaagc
     3301 ttcacgacga gcacgagaag cacaatctct gcaagcgcat caggctgttg gcacacgata
     3361 agtccctcag ctgctccctg ccttatgatt tcaccggtca ccggctttag ctcggtaatc
     3421 aggtaattga tgtatttgtt gtgcaaaaat gcattctcct gtacaccatt tccggcatcg
     3481 gactggattt ttaaaaaggc ggcagaggag ttgcggcatg cctgaaatat catcgccatg
     3541 cgcacaaacg gagatatatc ggtgcgcttt gcaagctcct ttgcggtggc aagcggctct
     3601 tcgtagcttt ttgccataag tgcatcaaag atagcctcct ttgatgaaaa ataataatat
     3661 atgctaccct tgcctatccc ggctgtctgg gcgatttcgc tgacagatat ctgggatatt
     3721 ttttttgttt ctaaaagggt tcttagcgct tcaagtatct tctcgtattt ttccgaattt
     3781 ggtcttgtgg aatgtggcat agtggctcct cttttattag ttacgttcat tttaatatat
     3841 attatcacaa tccatatgat tcttccatga aattttaccg gggtaataga taatgatatg
     3901 atacgtttgg atagtttatg aaaaaataac agaatagttt gaaaacgaaa acatttatat
     3961 tgaccgccgg tcgaaaaagt gatattgtaa tgaagtaaca agttgaccgg tggtcgaaaa
     4021 ggagaattga tatgacttat gcagatatgt tttcaaaggt aaagggactg tttatggagt
     4081 ctgatgtgag cgatataagc gagcaccttg cttttcagtt caatataaca ggagaggcag
     4141 agggaatttt ttatgccgag gtaaaggatg gtgtgttggc agtggagccg tatgagtatt
     4201 ttgacaggga tgcgattttt atatgcagcg cagagactct ttttaagctg gcagaaggaa
     4261 ggattgatcc gattcttgca tttacaacag gcaagctcaa ggtggaggga aatattgata
     4321 aggcacttcg cctgaagcag attatagaca gtaagaaggc gtaggtgatg aggatgaagt
     4381 ggggaataat tgcaggtgtt ttaggtggta ttgctgcagc agaggcaggc ggcagtgctt
     4441 atttctacag gaggaccatg atgcgctaca atgccaagaa ggagcgcacg atgaagatgt
     4501 cgggggttga ctgggagagc tattacagct ttatgaagcc gcacggagag tggatgagag
     4561 cacagactca tgaggatgtg tggattaagt ctgatgatgg attaagactg catgccacat
     4621 attttcctgg gatagatgga ggtaatccgg ataaagccgt catatgcttt cacggctaca
     4681 caagtgaagc tatgtctgat tacagcagca tatccaatta ctatttaaag aagggctata
     4741 gcatgctgct tgtagatgcg agggcacatg gacaaagcga gggtaaattc ataggcttcg
     4801 gctgcaagga ccgctatgat gcgcttaagt ggattgactg gatgattaag aaggcaggca
     4861 atggcataag gattgttttg atgggaaatt ccatgggagg tgccactgtg cttatggcaa
     4921 gtggacttaa tctgccggag caggtgaagg gaattgtgtc agactgtgca tttacctcgc
     4981 ctaaggcggt atttacacat gtgcttcaca gcatgtacca tctgccggct tttccgatga
     5041 ttcagattgc agattttgtg aacagaaaaa tggcagggta tgggcttgat gaatgcaatg
     5101 ctgcaaaaga ggtgcaaaag gcgaagctgc ctattttgtt tatccatggt gataaggata
     5161 catttgtgcc atgtagcatg tgtgatgagc tttatgcaag ctgcgcgtca caaaagacaa
     5221 agctgatagt aaagggtgca ggacactgcg agagctatta caagaatacg aaagctttcg
     5281 aggacgcact tgataaattt ttggaaggag ttatgagatg atgaagacga gaaagggaca
     5341 caacgtatat ccgattacgg ttgcacaaaa atttcatttg tactatgcaa agtactgtcc
     5401 gaatatggca gtactcaata taggtacaag cctgacaatc ggcacggagc ttgactggaa
     5461 cgtgctaagg gattcaatca actacgcgta tgccagaaac gaggccatgc gtatccgctt
     5521 tacaagggat aaggatggag agtgctatca gtatattgcg gatgtggatg aggattttaa
     5581 agagagaact gtcgatttca aggattttac ggatgtaacc atggaggagg cagagaatga
     5641 gatgcaaggc tggacacagg tgccgtttga gtttgaggac tctcctatga ccaaaatcgt
     5701 gatgataaag atgccggatg gattcaatgg agtttacttc ttaggtcatc acatggtggt
     5761 tgatgcgcag tcacttattg catttttaaa ggatattatt gagatatact gtaatgccat
     5821 gtatgaggga gtaccttttc caaaggatat gtgctcatat atcgagcagc ttaaaaagga
     5881 tctggcatat gaagccggaa gtaaggcaca gcttagggac agagagttct ttgagaagct
     5941 tatcagacag tcggagccaa tctacaacgg aatcgacggc acagcaaagc ttgatgcggc
     6001 aagagaactg atgcatgata acaagctaag gagtgcattc aatgcatcgg atgatgtcac
     6061 ctcagcgctt gatatctttc atctggaggc tgagccgaca aagcgtctta tggatttttg
     6121 cgagaaatat catatttcgc ttgcttgtct gcttctcatg ggaatcagga cgtttttcca
     6181 gaaggaaaac ggcttcgatg atgtctccgt aaacaatgcc atcgccagac gtgcgacact
     6241 taaggagaag aagtcaggcg gcacacgtat tcattcgttt ccgtttagga cgtgcttttc
     6301 aaaggatgta cgctttattg atgccgtgta taccatcagg gataagcaaa acgagctgtt
     6361 ccgccatgca aactacaacc cgacagagta ctttgcactg cgctcaaaga cctatccgca
     6421 gccaaaggca gggcttacat atgagccgat gtcgcttaca tatcagccta tgaccttaaa
     6481 ggaaaagggc ttaaacgacc tgggagatat aaaatacaag actaagtggt acccgaacgg
     6541 catgactaca caggctatgt atctaacggt aatgcaccgc ccggaggaca acggactgga
     6601 tttcagcttc gagcatcagg tcaaggcagt ctcaagaaag cagcttgagt atatgtacta
     6661 ttacctgtgc aaaatcatgt tcaagggagc agaaaaccct gagcttacaa taggcgaaat
     6721 cattaaatta gtgtagtgga ttaggaaacg tattaggtaa aaggagatta ttatgtttga
     6781 tgaattagtg gaaattatct gtaattatgt tgatgttcag ccggcagatg tgcacgaaga
     6841 gtcacgtttt atggaggatc tgggcttcac gtcatttgac ttcatgagca tgctcgggga
     6901 gatagaggat acctttgatg tggagattga gcagacaaag gctgcagaaa tccgcacagt
     6961 acaggaagca gtcgattatc ttgagacatt aaaagacgca taatgggtga agataggagg
     7021 aaatatgtta tttcacacaa ttccggatat tctttcatat gcaaatgaag cttacggcgc
     7081 ggatgacgcg ataagatgga agaaatccaa aaatgagata gagtcaagaa cctacagtga
     7141 gcttaagaat gatacagaca gctttgccaa tgccatcgaa aagcttggca aaaaagggca
     7201 gcatatcgct gtaataggcc cttcatcata tgagtggata gtatcatatc tggcaatcac
     7261 agaaagcgga agtgttgcgg ttccaatcga tgcatcgctt ccggcagcgg atatatgtga
     7321 gcttttggac agagccagtg tgaggatgct tatatttgat gaggcacgtt cggatgtggc
     7381 tgaggccgca gctaaaagct gtcatgatat aaatgtgtat gtgtctatga acagtacaga
     7441 gcattgtcca caggtgcttt cgtttaaagg gcttatagat gacaacaggg gaagctacga
     7501 gccggcagtg gcagaggatg cgctttgcac tatcatgttt acgtccggaa ccacaggaaa
     7561 gagcaaggga gtcatgctga cgcagaacaa cctggctgaa aatgccacat gccttgacat
     7621 gaaaattgga cctcacacgg taattttgtc tgtgcttccg atacaccatg catactgtct
     7681 gagcatggat attctgaaag gaatttcact gggttcggta atctgcatca atgattccat
     7741 catgcgcatg gcaaaaaata tacagctttt tactcccgac atgattctta tggtgcctct
     7801 tatgattgag acatttgcca gaaagctcga ggaggtaagg gcagcaggac ttcctgcaga
     7861 gccggtccgt aagaaaatgt ttggagagag gcttcataca atctgcagtg gcggtgcgta
     7921 cctaaatcct gattatgttg atttatttgc agagtttggt atcaccatat tgcagggcta
     7981 tggcatgacg gagtgttctc cggtcataag cacaaatcta agctgggata tccgtaaaaa
     8041 ctcggtaggc aagctgatgc caaactgtga ggcaaaaaca gttgacggtg agctttttgt
     8101 ccgcggtaca agtgtgatgc agggctatta taagatgcca aaggagactg aggagacact
     8161 gtcggacggc tggcttcaca cgggtgactt aggctatgtg gacgaggatg gatatattta
     8221 tctgaccggc agaagaaaaa atctgattat caccaaaaac ggtgagaatg tgtcaccgga
     8281 ggagcttgaa aacgctctga gcgtaaatca tctcataaag gaaatcatag tgcgtgagtc
     8341 tgaaggagtg attgaagctg agattttccc ggacagagag tatgctcaaa atacaggcat
     8401 cgccgatata aggccagctc tgcaggcgct tatcgatgag tacaatgtaa atgtacctgc
     8461 gtacaagcgt atctatagcc ttaaagtaag ggagagtgag tttgaaaaga cggcttcacg
     8521 taagataaag agaagctgat atataaagag gaagccgatg catgtggctt gcatcggcta
     8581 tatatgaaaa agattagtat gagtattgcg taattaatat gttttgttgc ttttattttg
     8641 tatctttcgt ttatgatgtt agtatacaaa tcaattgtga aaaccgtgtg ttttgaattt
     8701 cacttttctg taaacaaaat atgaacaaac tgtgcagtcc taaggtacca taaaaaatcc
     8761 ttagacaata tcatgatttt gtgtataatt atagtatcgg tatttgttat tgcatatagg
     8821 atataatttt atgcataatc aaacagcgat attatttttt ttacaagggg aaaaagatga
     8881 aattattaag ttttgcaatc ccatgttaca attcaaagga ttacatggag cattgtatag
     8941 agtctatcct gccgggtgga gatgatgtgg agataattat tgttgatgac ggttcaaagg
     9001 atgagacagc agcgatagct gacagatatg cagccgaata tcctgacata gtaaaggctg
     9061 tacaccagga gaacggtgga catggagagg ctgtaaacac aggtctcaaa aatgccacag
     9121 gcaaatattt caaggtcgtt gacagcgatg actgggtgga tttggattca tacaagaaga
     9181 tactggacaa gctaagggag tttgagcagg agaatacaca gattgatatg cttcttgcca
     9241 actatgtcta cgagaaagag ggagcaaagc gtaaaaaggt tatgcgtcag accggctttc
     9301 cgcgcaatga gatatttacc tggagtgata taaagcatat ttataagggg cattacatac
     9361 tgatgcattc ggtaatatac aggacagagc ttttgagaag ctgtggactc aagctgccaa
     9421 agcatacctt ttatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgatg
     9481 tactatctgg atgtggactt ctacagatat tttatcggtc gcgatgacca gtcggtcaat
     9541 gagcaggtga tgatacgccg tattgaccag cagattcgtg taaataagat tatgtttgac
     9601 gatgtcaaat tgcatgagat aacaaatgag atgtgcagga agtatatgta cagctatctg
     9661 gagattatta cgactatttc tactattctg gcaattatct caggcaccga tgagaacatg
     9721 gctaagaagg atgaactgtg ggcatacatg aaggagcatg atgaagaaac ctacaagaag
     9781 ctacgccatg gcgtcatggg acagttaatg aacctgccgg gcaagggagg cagaaaggta
     9841 gctatcggtg cgtataagct gtcacagaag gttgttggat tcaattagac ggatgtgaaa
     9901 taaggagtat tatatggatg aagtatataa gcaaattgtt aatatagcaa aaaaatataa
     9961 tacaaaaaaa gtagtgctgt ttggttcacg cgccagaggc acgaatcatc ctaaaagtga
    10021 tattgatctg gccatttatg gatgtgagga ttttggtgat ttatcggatt gtcttaatga
    10081 ggagttgtgg tctttactaa agctggacat aataaatatg gatgataagc atatttcacc
    10141 ggtactggtg acagaaatag aaagggatgg gagggtattg tatgaaaaaa tttgaccagt
    10201 atgtcagcca cttgaggata ttgtcaaggg catttgatga agacttgaca aatgatttta
    10261 ttgtgagcgg tataattgat aaatattata tacaatttga gctgggctgg aaggtcttaa
    10321 aggaactgtt acgatatgag ggcgctaatc aggctgcaac aggttctcca agagaaatta
    10381 taaaaactgc atatgcgtat tatgatttta ttgatgagag tgtctggctt ggaatgttaa
    10441 gagacaggaa tgatacaact catatataca atgaggaagc tgcacgtcag cttgtgaata
    10501 aggtgcttga cagttatatt catgaatttc aggtgctgga aatgaaaatt aaagaaagat
    10561 attcagatga ggtattatca tcaattaatt aaagtaaaga ccacagagca aaacagagac
    10621 tctgtggtct tttaatattg caattaaatt actctccaag tgtgagcatg gtatctatgc
    10681 agcccttggc ctttgttctt atatcgtcag gcagtatgat ttcttctccg cctgctccct
    10741 taacacaatt gtatacatcc atcagggtag tgagcttcat attgtggcat acgcagtctt
    10801 ttgagagcgg atagaagctc ttttcagggc actcatactg taagtgctgc agtatgctgt
    10861 tttcagttcc gatgataaat tccctgtctg tgctgttttt agcatagtca atgatgccgg
    10921 tggtgcttcc gatatagtct gcagcctttg aaacctctgc aaggcactcc ggatgtacga
    10981 gaagctttgc agcaggatga gccgcttttg ctctctcaac atctgctatt gacatgcgca
    11041 ggtgagtagg acagccacca tgtacaaatt tgaaggtctt ttccggaacc tgttcctcaa
    11101 cccatgcgcc cagattgcag tcagggatga aaagaatctt gttattgtca atttttttaa
    11161 caatttttac agcggatgaa gatgtgacgc acacatcgca tatggtttta agctctgatg
    11221 tggtgtttat gtaggcaacc actgtatagt cagggttctc ggcctttaac gattgtaaga
    11281 gctctacatc gagctgctcc gccataggac agcctgctgc gctgtttgac aaaaatacat
    11341 gcttgtcagg agataaaatc ttgcaggtct ctgccatgaa gcggacaccg cacatgagca
    11401 cattcttctg tggtgctgtg gcagcctgct tgctgagtcc atatgagtcg cctgtgaagt
    11461 cggcaacctc aagtatctcg tgactctgat aagcgtgagc taatatacag atatcatttt
    11521 ccttctttaa tcggataatc tcatcctgta attctcttgt tgtcatcata atatgatata
    11581 cctcttgaaa ttcctagtgt gattgaagca cagttatatc aatgtaagcc ccttttggat
    11641 tttacataaa gaaccggcca aaataaataa gttacttgtc attatactta gctgtaaacc
    11701 agctgtcaac acaaaagttt tacaggtgtc ttgtcagttg taaattttag tgttataata
    11761 cgcccattac agtaaatcgc atccaggggg atgttgctaa agaagcggct acatcggtgg
    11821 atgcaggtaa ataaaagagg taacatatta tgaatacaga tgtacttatt gtgggaagcg
    11881 gctgctcagg tctttattgt gcgcttaagc ttccaagaga tataagaatt acactgataa
    11941 caaagtcaga tctggagagc aatgattcat ttctggcgca gggcggcatg tgcatgctta
    12001 aagagcagtc tgatttcgac agcttttttg aggatacctt aaaggccggc cactacgaga
    12061 atgacaagga gtcagttggg ataatgataa actcatcacc tgatgtggtt aaggatcttg
    12121 tttcatacgg agcagatttt gcgagaaatg atgacggctc acttgcatat acgagagagg
    12181 gagctcattc acacaacaga attattttcc atgaagacgt gaccggaaaa gagataacaa
    12241 gcacattgct tgcacaggtt aagaagcttt caaatgtgac actcatggag tatacaacga
    12301 tggttgatat catcgaaagg gataacaaat gttacggcgc catcatcaga aaacaggatg
    12361 gcatgctgga aaaggtgaca gcggcataca ctgtcatggc ctgtggcgga gttggaggac
    12421 tgtacagatt ttcaacaaat ttcagacatc tgacaggtga ctcgcttgcc attgcaaaaa
    12481 agcatggcat agagcttaaa aacccggact atgtgcagat tcacccaacg accttttaca
    12541 cgaaaaagca tgaggacaga agctttctca tttcagagtc cgtaagaggg gagggtgcta
    12601 agcttttaga caagaatatg aacaggtttg tcgatgagct cttaccaaga gacgtgctga
    12661 ccggcaaaat ccgtgagcag atgaaaaagg acggcacaga ctttgtctgg gaggatttgc
    12721 gcaccattcc aagggatgag cttgtatcac attttccaaa tatcattgaa cattgcaagg
    12781 agatgggata tgatgtcttc aaggagccta ttcctgttgt tccggcgcag cactatttta
    12841 tgggcggcgt caaggtgaat caccacagcc gcacatccat ggaatgtctc tatgcggttg
    12901 gagagaccgc atgcaacgga gtgcacggaa gaaaccgtct tgcaagcaat tcactgcttg
    12961 agagcctggt attcgcaaag cgtgcagcag gacagatggc ggagcttggc tttgtaaatg
    13021 atgagatagc agccaaaaag gccgcagata gcatcgacct cgcagactac agcgatgaaa
    13081 aagctgtaga gagagaatac aggaagctcg ccatggaggc tatagaggga cgcaccagcg
    13141 tagatgcaga agaaactgtc gaatcaggca ttgcctatat tcaggcataa aagtaggttt
    13201 cgcactcgta aatctgcgaa gccggagagt acacagtgtg ttccgaagaa aaatatgaac
    13261 tgccacgggg ttccaacagg tcaaatgaca gtttagactt gcaaactgcc ataacctatc
    13321 cgtaaataat cccgtggct
//
LOCUS       DPUF01000073            3585 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16205, whole
            genome shotgun sequence.
ACCESSION   DPUF01000073 DPUF01000000
VERSION     DPUF01000073.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16205"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="DHV81_02710"
     CDS             <1..1614
                     /locus_tag="DHV81_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HCI93429.1"
                     /translation="QLKEMIAMNASDYFPVDLSQYKLVHQIIEKIDRPEEKKLRLSVI
                     AVPNDLILSYGALAADCRLQLVALDYSGNAIKQLMRREIPGDVKVTVKVDEVSTTLTV
                     MDGSMVKLQRNVNHGLADAIEAIQDSELFGEYLSFTDAVDVARRKTCLAIRPDGTVPD
                     EPAGGGMDPMGHEIDSERFKKLRLNVSYSLSNLISSLGRVIDYYQSRNSDTPIERIFL
                     IGLGADFSGLSKLLTNELNVKVVPLQQFDGIHVAKSINLAEAKLAEYFNCVGCSLSPL
                     DILDSKKNKGIGATIMAGTAAGAMPSGGAQAGMQGKPPVGPDGKPLPVPAGEEAEKPV
                     SFAVQLLIFGLCVVVAVGITAYSIFSNLVLTSDNMTLKARVSDLSYAQNIYDVYNQTK
                     KDYDWTKLLESASSNKNDEITGFVDELEQKLPSEAKLVNMSVTTDGVSMTIKTGSKDI
                     AADVIAQLRTFESIMVSNVSTITEEVDETGASKVQFTVDCTYVKADDSAASESADGTG
                     SEPADTSGDTSSQAQTDSNSSSDTDSTNQ"
     gene            1626..2432
                     /locus_tag="DHV81_02715"
     CDS             1626..2432
                     /locus_tag="DHV81_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilO"
                     /protein_id="HCI93430.1"
                     /translation="MSTRDKKLLIILAGVAILALTYFLIFIPQSNKRDSLKSENMNLK
                     SQYNQLSALAAKADDYTEKTKDMSDAMQQVYDNFPSYLQIENGIMDAVAIEKSTNTNI
                     TELTVGSPTAIDVNNPTGEAGTDSSSGSSSDSSTTDSSNADSSQSSQTADGNSSQSQT
                     GDGSAADGSTSTGSAKSTVTGYQLYDVSTVMAFKTEYHGLKAMLGEVIGSSQKKTIST
                     LNVSFDDSTGKLTGEMLVDSYFLYGLDKPYEAPYIPAVPHGTNNLFGTTN"
     gene            2552..>3585
                     /locus_tag="DHV81_02720"
     CDS             2552..>3585
                     /locus_tag="DHV81_02720"
                     /inference="COORDINATES: protein motif:HMM:PF07963.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93431.1"
                     /translation="MRKLKNDDRGVTLVEIIVSIAILAIIVLPFLNAFVTATKTNVKA
                     KNEMNATHLATNIMEGIEKNSMKTLAYQFNYPSEGFDVADGFNISDGSSACELLKKSG
                     KFDNVKRLEDISAEIVNKDDVITSCIHKTDASAKISDTSLWNFRESDAHKYYFYMSGV
                     QSGTKKYNALVTVDAKSDAESDNKVKKYNMDEVADMSAMDANFDCMSADKYSATNIIY
                     AFNNMPGVGGITQQDIKRTITIDIEKYGASSNKATKVTVSYSYSINKNGVRKTFPDPN
                     SALKDDYTMVIYDNSSDTVNHNLRNVYLFYNPWYTSTGTLYNTCNDVIIINNKDKLDC
                     TVNIVKQKTIS"
BASE COUNT         1090 a          668 c          885 g          942 t
ORIGIN      
        1 cagcttaagg aaatgattgc aatgaatgca tcggattatt ttccggtgga tttgagccag
       61 tacaagctgg tacatcagat tatagagaag attgacagac cggaagaaaa gaaattacgt
      121 ctttcggtta ttgctgtgcc taatgatctt attttgtcgt atggtgctct tgcagctgat
      181 tgcaggctgc aacttgtagc acttgactat agtggaaatg ccatcaagca gctcatgaga
      241 agagagattc ccggagatgt gaaggtgact gtcaaggtgg atgaggtttc taccacactg
      301 actgtaatgg atggaagcat ggtaaagctg cagcgcaatg tcaaccatgg acttgctgat
      361 gctattgagg cgattcagga tagtgagctt tttggagagt atctgtcatt taccgatgca
      421 gttgatgtgg cacgcagaaa gacctgcctt gcaataaggc ctgatggcac agtacctgat
      481 gagccggcgg gcggcggcat ggatcctatg gggcatgaga ttgattctga gcgctttaag
      541 aagcttcgcc tgaatgtatc atattcactt tccaatctga taagcagtct cggacgagtt
      601 atagactatt atcagtcgag aaactcagat actcctatcg agagaatttt cctcattggc
      661 ttaggtgctg atttcagcgg actctcaaag cttttgacca atgagctgaa tgtcaaggtg
      721 gttccgcttc agcagtttga cggtatacat gtggcaaaga gcataaacct cgcagaggca
      781 aagctggcag agtatttcaa ctgtgtcgga tgttcactga gcccgcttga tatacttgac
      841 agtaagaaaa acaagggaat cggtgctacg attatggcag gaacagctgc aggtgccatg
      901 ccatccggcg gcgcacaggc agggatgcag ggaaaaccac ctgttggtcc tgatggaaag
      961 cctcttcccg taccggcagg ggaggaagcc gaaaagcctg tttcttttgc agtgcagctt
     1021 ctcatttttg gcttgtgtgt ggtagttgca gttggtatca cggcatacag cattttttca
     1081 aatcttgttt taacttcaga caatatgacg cttaaagctc gtgtttctga tttgtcatat
     1141 gcacagaata tatatgatgt gtataaccag accaagaagg actatgactg gaccaagctt
     1201 cttgagagcg cctcatccaa caagaatgat gagataacgg gcttcgtgga tgagctggag
     1261 cagaagcttc cgtcagaggc aaagcttgtg aatatgtctg ttacgactga tggtgtttcg
     1321 atgaccatca agacaggcag caaggatata gcggctgatg taattgcaca gcttcgtaca
     1381 tttgagagta tcatggtatc caatgtgtca accatcacgg aggaggttga tgagaccggt
     1441 gcttcaaagg ttcagttcac agtagattgc acatatgtaa aggctgatga cagtgctgct
     1501 tctgaatcgg cagatggcac aggcagtgag ccggctgata catcagggga cacgtcctca
     1561 caggcacaga cggatagcaa ttcatcatca gatacagaca gtacaaatca gtagggggtg
     1621 ttaggatgtc aacgagagat aaaaagttac ttattatatt agcgggtgtt gccattttag
     1681 cactgacata ttttttgatt tttattccac agtccaataa gagggattca ctcaagagcg
     1741 aaaatatgaa tctgaaatca cagtacaatc agctttcagc acttgcggca aaggcggatg
     1801 actacacaga gaagacaaag gatatgtctg atgccatgca gcaggtatat gacaatttcc
     1861 cttcatattt acagatagaa aacggcatca tggatgcggt tgctatcgag aaatctacca
     1921 atacaaatat tacagagctt acagtcggct caccgacagc tattgatgtg aataatccga
     1981 ccggtgaagc cggtacagat tcttcgtcag gaagcagttc agacagcagt accacggata
     2041 gcagtaatgc agacagttcg cagagctctc agaccgctga tggcaacagc tctcagtcac
     2101 agacaggcga cggcagtgca gctgatggca gtacatcaac aggctctgca aagtctacgg
     2161 ttacaggtta tcagctttat gatgtcagca cagttatggc attcaagaca gagtatcatg
     2221 ggcttaaggc tatgcttgga gaggtgattg gttcatcaca gaagaaaact atcagcacgc
     2281 tcaacgtgag ctttgatgat tcgacaggca agctgacagg tgagatgctt gtggattcat
     2341 atttcctgta tggacttgat aagccatatg aggcaccata catacctgca gtaccacatg
     2401 gtacaaataa tctgtttggt acaacaaact aactgattat tataatatta ggttattaga
     2461 ttagcgggtt attgagaccg taagtgataa atatcaaatg ttagattatt gagttatgag
     2521 ataacaaaat acaagagggg aggaagcaga tttgagaaag ctaaaaaacg atgatcgagg
     2581 agtaacactt gtggagatta ttgtgtcgat tgctattctc gcaataatcg tgcttccttt
     2641 tttgaatgca tttgtcacag ctacaaagac caatgtaaag gctaaaaacg agatgaatgc
     2701 tacacatctg gcgacaaaca ttatggaggg tattgaaaag aactccatga agacactggc
     2761 atatcagttt aactatccgt cggagggctt tgatgtggcg gacggtttta atatatcaga
     2821 tggaagcagt gcatgtgagc ttcttaagaa gtcgggtaag tttgataatg taaagaggct
     2881 tgaggatatc agtgctgaga ttgtaaacaa ggacgatgtc atcactagct gtatccacaa
     2941 gactgatgca tcagccaaaa taagcgatac atcgctttgg aatttcaggg agtcggatgc
     3001 gcacaagtat tatttctata tgtctggtgt gcagtcaggc acaaagaaat acaatgctct
     3061 tgtgacagtg gatgcaaaat cggacgcaga gtcggacaat aaggttaaaa agtacaacat
     3121 ggacgaggta gcggatatgt cggccatgga tgcaaatttt gactgcatga gtgcagacaa
     3181 gtattctgct acaaatatta tatatgcatt taataatatg cctggtgtag gcggcatcac
     3241 tcagcaggat atcaaaagaa ctatcaccat agatatagaa aagtatggag cttcgagtaa
     3301 taaggctacc aaggttacgg tgtcgtattc atattctatc aataagaatg gagtaagaaa
     3361 gacttttccg gatccgaata gtgcactgaa ggatgattat accatggtga tttatgataa
     3421 ttcttcagat actgtgaacc ataatctgag gaatgtctat ctgttttaca atccatggta
     3481 cacatctaca ggaacacttt acaatacctg caacgatgtc attataatca ataataagga
     3541 caagctggac tgcacggtaa acattgtaaa gcaaaagact atatc
//
LOCUS       DPUF01000074            4263 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_35854, whole
            genome shotgun sequence.
ACCESSION   DPUF01000074 DPUF01000000
VERSION     DPUF01000074.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_35854"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            703..1536
                     /locus_tag="DHV81_02725"
     CDS             703..1536
                     /locus_tag="DHV81_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI93432.1"
                     /translation="MKKLLFFDIDGTLVDFGNSTMSPSTADALINAKQNGHMIFLCTG
                     RSYNQIYPSLKAFDFDGVVAAAGGYVTVGDKVIAHHVYGQNLLQKVLDTVGDNDTGLI
                     FQTKDKSITNHKWADKFISAFSKQFDMHVIQDNPTFKDIVIDDELSSFSNRYADVEST
                     IYCNCNYHIDDLRKLLGDEFVVTLSSFKEPEPYSGEITLRGVNKATGIRDVVEFLHMS
                     QADTIGFGDGQNDFDMLRYCDVGVAMGNSSDEVKAVADIVTDDIKEDGLKNAMVHLGL
                     V"
     gene            complement(1556..2011)
                     /locus_tag="DHV81_02730"
     CDS             complement(1556..2011)
                     /locus_tag="DHV81_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93433.1"
                     /translation="MKFRTENEFTHFKFSDVHVSDIMMSFGTFKICLDNVIIKADNSK
                     NRDIRDMRTNGLILKLSDANIISFIREGFKTYDADGNLKSTTADEEIDEADYIDTFNN
                     FLDGYAYLIEKENENYTFVFDGTDERSYTLIVSASKDECEWDRFMNIEA"
     gene            complement(2079..>4263)
                     /locus_tag="DHV81_02735"
     CDS             complement(2079..>4263)
                     /locus_tag="DHV81_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HCI93434.1"
                     /translation="EALEKFCWDYEEKCIPRDEVYMEKTGNLLVGKVLIDTYLHNNPL
                     LSMQSKILMLNEIIYSKYENEVLGKEVKFPAKERRVLDKKYKTYFGKDDWKGSVYDFY
                     RDFLLSQKEKEYDIDIPKDSFDVYDLAALAYIYKRIKETDPVREASHVVIDEAQDFGM
                     MAYCCLHYCLRNCTYTIMGDTSQNIHFEYGLNDWEDLKKLILTGTYDAFGLLRKSYRN
                     TVEISEFATEILRHGDFAIYPVEPIIRHGNAVRIEEYANVRSLISASFDTIKGWQSEG
                     YETIAVVCRDEAEALKVSAELKKHIEIADDDIETAQFGAGVMVLPVAYTKGLEFDAVL
                     LFDPSERKYPADDSHVKLLYVAATRALHELAVFHRGRLTPLIADPAPSNRHQKEFSAE
                     PLTKAKEYEKQQLTEKEIEEQKRVDGRRDMDEREYFGPSRIALKPEQLTNKAENEKLD
                     LSAFVKKDRENQTQCTATDMANKIKIKEVSKAAKKSSLPLNPSPYAYGSIPDNDILHV
                     KGHSKGKFAVKWLKKGKSHVEIATADGTLYVIPITPEIVRVIFVKGIGVKPHKTYWKQ
                     KADTAFKWVAKESKSLIEIQTEKLILRIEKKNGAIQYFDADRKLLVSENATEPRLLNN
                     GECYTFFDWDKSEKLKSKGILATDLTDLTNKARYISFGGRQQRLPLVVSNKGYGIAAA
                     SSRTALFCNIKMYGQYIFVDGDTQSDYYFIGAGSVGHTLELYGTL"
BASE COUNT         1130 a          968 c          802 g         1363 t
ORIGIN      
        1 gcacccttta ctttgatgtt tttgccctcg tagtcgcctg caagaatttc tgcgataccg
       61 aggtcttcct tttgaactgc tcctgttaag aattccatat ttttgtgctc ctttgatttt
      121 ttaagcggga cggaaaaagc cccggagcat atcaaattaa tgaaatgctc cggggcgaaa
      181 attctaaaaa tattccgcgg taccacccgc tttgagaaaa aggactgttc ctgtttctcc
      241 actcttatcc gtaacgtgga tgaatcgtat ctgcctactt ttgatatata aaaagcttaa
      301 ttcacatacg ataaacctat ctatgcaata aaccatatca atcgttcaac agatctgctc
      361 cggtagtgtt cctgcaatca gctcttccaa acagcgctct cagtcggtga cgctgtattc
      421 ctgtcggtgt ctggtgatcc aagtctcctt caacgcattt attgtttttt gttgtttaac
      481 gttataacac aataaactta ttatttcaac ccatcatagt caaaacttaa gaaaataaag
      541 tataggaata tattatagtt tttcttaatt gtggttataa cagagccttc ttttgcaaca
      601 attattaaat aaatttaggc tattccctat tgcctttatt gaattcgtat tgtagagtat
      661 atctggtata tgtaattaat aataatgagg caggtaacac ttatgaaaaa actattattt
      721 tttgatatag acggcactct cgttgatttc ggcaatagca ccatgagccc atcaaccgct
      781 gatgctctaa taaacgcaaa acaaaacggt cacatgattt ttctttgcac aggacgtagt
      841 tacaatcaga tttatccatc attaaaagcc tttgattttg acggcgtggt ggctgctgcc
      901 ggcggctatg tcactgtggg tgataaggta attgcacatc atgtctatgg gcagaatctg
      961 ttacagaagg ttcttgacac tgttggtgac aacgacacag gacttatctt ccagacaaag
     1021 gataaaagca tcaccaacca caagtgggcc gacaaattca tatcagcatt ctcaaagcag
     1081 tttgacatgc atgtgataca ggacaacccg acatttaagg atatcgtgat tgatgatgaa
     1141 ttatcaagct tttctaatcg atacgctgat gttgaaagca ccatctactg caattgcaac
     1201 taccacattg atgatttaag aaagctcctc ggcgatgaat ttgtagtaac cctgagcagc
     1261 tttaaagagc ctgaacctta cagcggtgag attacattgc gcggtgtcaa caaggcaacc
     1321 ggcatacgtg atgtggtaga gtttcttcac atgagtcaag ctgatacaat aggctttggc
     1381 gatggccaga atgattttga tatgctgcgc tactgtgatg tcggtgttgc catgggcaat
     1441 tccagcgatg aagtcaaggc tgttgccgat atagtgacag acgatatcaa agaagatggg
     1501 cttaaaaatg ccatggtgca tcttggactt gtctaaaaat gcaccatggc atactctatg
     1561 cctcaatatt catgaatctg tcccactcgc actcgtcctt tgatgccgat acaatcaagg
     1621 tgtacgagcg ctcatctgtt ccatcgaata cgaaggtata attttcattc tccttctcga
     1681 ttaaatatgc gtatccgtca aggaaattgt tgaaggtgtc gatataatct gcctcatcta
     1741 tctcctcgtc agccgttgtg ctctttaggt tgccatcggc atcatatgtc ttaaagccct
     1801 cgcgtatgaa tgaaataata tttgcatctg aaagctttaa tataaggccg tttgtcctca
     1861 tatcacggat atctctgttt ttagaattat ctgcctttat gattacgttg tcaaggcata
     1921 ttttgaaggt gccaaagctc atcatgatat cgctcacatg cacatccgaa aatttgaaat
     1981 gtgtaaactc gttttctgtc ctgaatttca tctgttatcc tccgtatata ctcatgccga
     2041 tattttatca gataattttg ctttgggcaa cttgcatttt ataatgtgcc gtacagctca
     2101 agtgtatggc ctacactgcc tgcacctata aaataatagt cgctttgagt gtcaccgtct
     2161 acaaagatat actgtccgta catctttata ttgcaaaaga gtgctgtgcg gcttgatgca
     2221 gcagcaattc catatccctt gtttgacacg acaagtggca gcctctgctg tctgcctccg
     2281 aacgaaatat atctggcttt gtttgtcaaa tcagtaaggt ctgtcgcaag tattcccttg
     2341 gatttgagct tttccgattt atcccagtca aaaaatgtgt agcactcgcc attatttaaa
     2401 agccttggct ctgtggcatt ttcgcttaca aggagttttc tgtcggcatc aaaatactgt
     2461 atggctccgt ttttcttttc tatccttagt atcagcttct ccgtttgaat ttcaataagc
     2521 gattttgatt cctttgccac ccatttgaag gctgtatcgg ccttttgctt ccaataggtt
     2581 ttgtgcggct tcactccaat tccttttaca aagattacgc gtactatctc cggtgttatt
     2641 ggtataacat aaagtgtgcc gtccgctgtg gcgatttcaa catgcgattt accttttttg
     2701 agccatttta ccgcaaactt gccctttgaa tgtcccttta catgaagtat atcgttgtct
     2761 ggaattgagc cgtatgcgta tggggaggga ttgagaggga ggctgctctt ttttgcagcc
     2821 ttacttactt ccttaatctt aattttattt gccatatctg tagcagtaca ttgtgtttga
     2881 ttttccctgt ctttttttac aaatgcagat aaatcaagct tctcattttc agccttattt
     2941 gtcaactgct caggctttaa tgcaatgcgc gacggaccaa aatactctct ttcatccata
     3001 tcgcgtctgc catcaacacg cttttgctct tctatctctt tttctgtcag ttgctgcttt
     3061 tcatattcct ttgccttagt aagcggctct gcagaaaact ccttctgatg cctgttggaa
     3121 ggagcagggt ctgcaataag cggtgtcagc ctgccccggt ggaatacagc aagctcatga
     3181 agtgcacggg tagccgcaac gtacaaaagc ttcacatgac tatcatcagc cggatatttt
     3241 ctctctgatg ggtcaaacag caatacagca tcaaactcaa gtccctttgt atacgcaacc
     3301 ggaagtacca tgactcctgc accaaactgt gcagtttcaa tatcatcatc tgctatttct
     3361 atatgttttt taagctcagc agacaccttg agagcctctg cctcatcacg gcacacgaca
     3421 gctatcgtct catagccttc actctgccag ccttttatcg tgtcaaacga tgccgaaatc
     3481 agtgagcgca cattggcata ctcctctatg cgcactgcgt ttccatgcct aataataggc
     3541 tccaccggat aaattgcaaa atctccatgg cgcagtatct ctgttgcaaa ttctgaaatc
     3601 tcaacagtgt ttctgtagct ctttcgaagc agaccgaatg catcatatgt gcctgtgaga
     3661 atcagctttt tcaagtcctc ccagtcattt aagccatact caaaatgtat attctgcgag
     3721 gtatcgccca tgatagtgta tgtgcagttt ctgaggcagt aatgcagaca gcagtatgcc
     3781 atcatgccaa aatcctgtgc ctcatcaata accacatgac tcgcctctcg aaccggatct
     3841 gtctccttga tgcgcttata aatataagca agcgctgcca gatcatacac atcaaatgag
     3901 tcctttggaa tatctatatc atattccttc tccttctgac taagcagaaa atccctgtaa
     3961 aaatcataca cagagccttt ccagtcatcc ttaccgaaat aggttttgta ttttttatca
     4021 agcaccctgc gctcctttgc agggaacttc acttccttac cgagaacctc attttcatac
     4081 ttcgagtaaa ttatctcatt taacatgagg attttactct gcatggaaag aagcggatta
     4141 ttatgcagat aagtgtctat cagcaccttt cccacaagca gattgcctgt tttttccatg
     4201 tagacctcat ctcttggaat acacttttcc tcgtaatccc aacagaattt ttcaagagct
     4261 tca
//
LOCUS       DPUF01000075            9243 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_11277, whole
            genome shotgun sequence.
ACCESSION   DPUF01000075 DPUF01000000
VERSION     DPUF01000075.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_11277"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="DHV81_02740"
     CDS             <1..159
                     /locus_tag="DHV81_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93435.1"
                     /translation="KINDYYKNKSAWRKSALLNIAHVGKFSSDRTIQEYVDDIWHLDR
                     ITVNMAGV"
     gene            176..949
                     /locus_tag="DHV81_02745"
     CDS             176..949
                     /locus_tag="DHV81_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HCI93436.1"
                     /translation="MAKTGISGSTLKLIAIFTMLIDHTAATVILQMINNGIGGQTLID
                     IYWVMRSIGRMAFPVFCFLLVEGFKYTHSREKYAARLFIFALISEIPFDLAINNTVLE
                     FKSNNVFFTLLLGLLAIMVLDWLKSVDKIEKSSSAVKWFFITLIRCIVMVSVVLVIMI
                     IAEFVFCCDYGAAGVGCIVMMYLLSLNREIAFAVAVILLGLFSGTIEFFALFMLIPLR
                     YYNGKRGISLKYVFYAFYPVHLFVLYLICRLTMAVTGNT"
     gene            995..2152
                     /locus_tag="DHV81_02750"
     CDS             995..2152
                     /locus_tag="DHV81_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endo-beta-N-acetylglucosaminidase"
                     /protein_id="HCI93437.1"
                     /translation="MKKLLKTILALVFAACCFMFAAKCLKAGGFTGAIADFPTAIHRA
                     VKGQMQTTTDYLNGEQTEYDESVFTGEYHYYYEKLSDDDKLIYRQIYAMMQKRMDGIS
                     LNTTDTDNVSRIQQFVLLDNPQFFYIEKSQYIVRNSIIFSPVYNMSESDIDNAISTIS
                     GYVSACMAGIDEGASDYDKAVHFYEYVIEHAEYEENSPHNQDMYSAVLGRTVCQGYAM
                     MFKYLCDRAGISSLIVTGTTSDANHAWNMVYLDGAWCAVDCTYGDGDYLGNGISYSWF
                     GVPLDVVKLTRTLDNEDMLPQEATVEDDYYYRNGLYFTSYDLKTLQGMTTSSNYITFK
                     FSDRETYDTACHSLFEKGDYKYLIPTARGGTITYMQEPNSLAVFIHIELGC"
     gene            2181..5681
                     /locus_tag="DHV81_02755"
     CDS             2181..5681
                     /locus_tag="DHV81_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCI93438.1"
                     /translation="MSFCHLHTHTEYSLLDGSNKIKEYVKRVKELGMNSAAITDHGNM
                     YGVVEFYKTAKANDINPVIGCEVYVAPNSRFDRETSHGDDRYYHLILLAENNTGYANL
                     MKIVSIGFTEGYYYRPRVDFETLERYHEGLICLSACLAGEIPRYIVRGFYDEAKEIAR
                     KYQECFGKDSFFLELQDHGIDDQKLVNQQLLRMSKELGIGLVCTNDVHYTYESDAEAH
                     DVLLCIQTGKKVSDEDRMRYDGGQFFVKSEEQMRALFPYATEAIENTQKIADRCNVTL
                     EFGNYKIPKYEVPEGYDSAEAFLTELCEKGFREKYIGCGEYSDEELKKIHADMDYELG
                     IIKTMGFIEYILIVWDYINWCRTHDCWVGPGRGSAAGSRVCYCTGITDIDPVKYNLLF
                     ERFLNPERVSMPDIDVDFEYAERYRAIEYVQEKYGHDSVTQIVTFGTMAARGVIKSVG
                     KALDFPYAETDRLAKMVPQELNITIDKALQMNPEFKGIYESDARVHEMIDMAKRLEGL
                     PNHTSVHAAGVVIYPGVASDYVPLGRANDGSPTAEYNMVQLEELGLLKMDFLGLRTLT
                     VLKDSVKNIKASRGIDVDIDHIDFNDKGTLDFIGTGKTEGVFQLESAGMQSFMKELSP
                     QSFEDIVAGISLYRPGPMDFIPNYIKGKNNPNEISYVTPELESILEPTYGCIVYQEQV
                     MQIVQKLAGYTMGQADNIRRAMSKKKQYVIDAERQSFVYGDKERGIKGCVANGIDEKA
                     ANSIYDSMVDFAKYAFNKSHAAAYAKISVQTAWLKNYYPVEFMAALMTSVIDNSAKAA
                     QYLYSCRDMGIKVLPPDINCGQGEFTAEGDHVRYGMYAIKSLGRPVIDQIIEERESGG
                     RFRTLSDFIERTCQKELINKRAIENLIKAGACDSLDGTRKQMAMVYASIVDRVQHDKK
                     STMAGQMSLFDLVSDEDRQSFELKYPNVGEYDAEEALAYEKEVLGIYLSGHPLEKYAA
                     ALKKNITANAIDFELEEESGAVKIKDNAHVVIGGMIATKTIKFTRYNQAMAFITIEDL
                     TGTVEVIIFPRDYERYQRYLQDDAKIFVIGHATVEDEQNGKIICERIIPFDELPKQLW
                     LKFDTMDDYKKNESRLLETIRESDGADEVIIYIADTKQMKKLGRNYSVNANNELMEQL
                     LEFLDKENAKIVEKNIEKLC"
     gene            5745..6716
                     /gene="pfkA"
                     /locus_tag="DHV81_02760"
     CDS             5745..6716
                     /gene="pfkA"
                     /locus_tag="DHV81_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCI93439.1"
                     /translation="MAKEINTIGVLTSGGDAPGMNAAIRAVVRQALSKGKKVKGIKRG
                     YAGLLNEEIVDMDSKSVSDTISRGGTILQTARCKEFVTAEGQQKGAEICKKHGIDAIV
                     VIGGDGSFQGAQKLAALGINTIGLPGTIDLDIACTDYTIGFDTACNTAMEAIDKVRDT
                     STSHERCSIIEVMGRGAGYIALWCGLANGAEEVLLPEKYDNNLEALIERVAHARELGK
                     QHYIIINAEGVGHSQEMAKEIEAATGIETRATILGHMQRGGSPSCKDRVYATIMGAMA
                     VDLLCEGKSNRVIAHKDGNFVDFDIDEALAMKKSVDEYMYDISKSLV"
     gene            6889..7680
                     /locus_tag="DHV81_02765"
     CDS             6889..7680
                     /locus_tag="DHV81_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCI93440.1"
                     /translation="MITSVSNKSIKNVQALLSKVKERKKQGVFVVEGPKMSFEAPYDW
                     VSAVYMSESFFNDDRFKEEKKRFLDKTEIVSDSVFKSMSDTQTPQGVLAVVKMPHYDI
                     DELFKGDKTELLVLESIQDPGNLGTMVRTGEGAGVSGIIMNRTTVDIFNPKTVRSTMG
                     SLYRVPFYITDDLPETIEYAKSKGVYLYAAHLKGKCSYDRPDYTGACGFMIGNEGNGL
                     SDEIADMADTYIRIPMEGAVESLNAAISATLLMYECNRQRRLLGE"
     gene            7682..7945
                     /locus_tag="DHV81_02770"
     CDS             7682..7945
                     /locus_tag="DHV81_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93441.1"
                     /translation="MRIWFKIYTDTHLMRDITIENDSDDTRTHKIFKSLEDACLAFDL
                     GKPIWLDSNVSDFKRHGKTRFTKDNFVEDIDFDFLEMHIIEED"
     gene            8410..9084
                     /locus_tag="DHV81_02775"
     CDS             8410..9084
                     /locus_tag="DHV81_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCI93442.1"
                     /translation="MEQEFAEGIDSWETMMFLYNSALKEVNTKLEILNDEFVHIHNYN
                     PIEYIKSRIKTPESIVKKLKKGGYESTIENMVTYVKDIAGIRIVCSFTSDIYRLAEMI
                     GRQNDLTVISVKDYMKHPKASGYKSYHMIVSVPIFLSNKVVDTKVEIQIRTIAMDFWA
                     SLEHKIYYKFEGNAPEYISRDLRECAGIVGQLDAKMLSLNEAILEAKEKQEQEREEKI
                     REDEHH"
     gene            9071..>9243
                     /locus_tag="DHV81_02780"
     CDS             9071..>9243
                     /locus_tag="DHV81_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93443.1"
                     /translation="MNIINVENITKSYTGRKLFSKASFYVQENEKIGIIGINGTGKST
                     LLKIVAGDEEPDEG"
BASE COUNT         2785 a         1693 c         2385 g         2380 t
ORIGIN      
        1 aagattaatg attattacaa gaataagagc gcatggagaa agagtgcact gctcaatatc
       61 gcacatgtag gaaagttctc ttcagacaga acaatccagg aatatgtgga cgatatctgg
      121 catcttgaca ggattaccgt aaatatggca ggagtatagg attttggaaa aagttatcgc
      181 aaagacaggt atcagtggca gcacgcttaa gctcatagcc atttttacca tgctcataga
      241 ccatactgcg gcaacggtta ttcttcagat gataaataac gggataggtg gacaaacgct
      301 tattgatatc tactgggtca tgcgttctat agggcgcatg gcctttcctg ttttctgctt
      361 cctgcttgtg gagggcttca aatatacaca cagcagggaa aaatatgctg ctaggctgtt
      421 tatatttgca cttatctctg agataccgtt tgatcttgca ataaacaata cagtgctcga
      481 atttaagagc aacaatgtgt tttttacact gcttttaggc ttgcttgcca tcatggtgct
      541 tgactggcta aagtcggtcg ataaaataga aaagtcatcc tctgcagtta agtggttttt
      601 tataacactg atcaggtgta tagtaatggt atcagtggta cttgttatta tgattattgc
      661 agagtttgtg ttttgctgcg attatggagc agcgggggtg ggctgtattg tcatgatgta
      721 cctgctttct ttaaaccgtg agattgcatt tgcggtggca gtcatactgc ttggactttt
      781 ctcaggtacc atagagtttt ttgcactgtt tatgcttata cctcttcgct attacaatgg
      841 aaaaagaggt atttctctta aatatgtgtt ttacgccttt tatccggtgc atctttttgt
      901 actgtacctg atatgcaggc ttactatggc tgttaccggt aacacctaac agtgtgaccg
      961 gcaacgatta cagttcattt aaatggggga atagatgaaa aagcttttaa aaacaatact
     1021 ggcacttgtg tttgccgcct gttgctttat gttcgctgca aaatgcttaa aggcaggagg
     1081 gtttacaggt gctatagctg attttcctac agctatacac agggcagtaa aggggcagat
     1141 gcagaccaca acagactatt taaacggcga acagacagag tacgatgagt cagtatttac
     1201 aggggaatat cactactact acgagaagct gtcagatgat gacaagctta tttacaggca
     1261 gatttatgcg atgatgcaaa agcgcatgga tggaatttcg ttaaatacta cagacactga
     1321 caacgttagc cgtatacagc agtttgtgct gctggacaat ccacagtttt tctacataga
     1381 aaagagtcag tatattgtga gaaacagcat cattttttca cctgtataca atatgtctga
     1441 gtctgatatc gacaacgcaa taagtacgat ttcaggctat gtgtcagcct gtatggcagg
     1501 tatcgatgaa ggtgcatcgg attatgacaa ggcagtgcat ttctatgaat atgtgataga
     1561 gcatgccgag tatgaggaga attctcctca caaccaggat atgtactcag cagtgcttgg
     1621 aaggacggtc tgtcagggct atgcgatgat gtttaagtat ctgtgtgacc gggccggtat
     1681 cagctcactt attgtgacag gcaccacatc tgatgcaaac cacgcctgga atatggtata
     1741 tctggacggt gcgtggtgtg cggtggactg tacctacggt gatggtgatt atctgggaaa
     1801 tggcatatca tacagctggt tcggagtgcc gcttgatgtg gtaaagctta cacgtacatt
     1861 ggacaatgag gatatgctgc cgcaggaagc gactgtggag gatgattatt attacaggaa
     1921 tgggctgtat tttacatcct acgaccttaa aacattacag ggcatgacaa cgtcatctaa
     1981 ctatatcacg ttcaagtttt cggacagaga aacctatgat actgcatgtc acagtctttt
     2041 tgagaagggt gactacaaat atctcatacc gacggcaaga ggcggcacta tcacatatat
     2101 gcaggagcct aattcgcttg cggtgtttat acacatagag cttgggtgct gatacaaaaa
     2161 taaaagaaag aggaatccaa atgtcatttt gtcatcttca cacccatacc gagtacagtc
     2221 tgctcgatgg gtcaaacaag ataaaagaat atgtaaagcg cgttaaggag cttggcatga
     2281 actcggccgc aatcacagac catggtaata tgtacggtgt ggtggagttt tacaagacgg
     2341 caaaggcaaa tgatatcaat ccggtcatag gctgcgaggt atatgtggca ccaaattcac
     2401 gctttgacag ggagacaagt cacggtgatg acaggtatta tcatctgatt ctgcttgctg
     2461 aaaataatac aggctatgcc aatctcatga agatagtgtc aataggcttc acagaaggct
     2521 attattacag gccgagagtg gattttgaga cacttgagag gtaccacgag gggcttatat
     2581 gcctgtctgc ctgtctggca ggagaaattc cgagatatat cgtcagaggc ttttatgacg
     2641 aagcgaagga gattgcaagg aagtatcagg aatgctttgg aaaagacagc tttttccttg
     2701 agctgcagga tcatggcatt gatgaccaga agcttgtgaa tcagcagctt cttaggatga
     2761 gtaaggagct tggaataggg ttagtatgta ctaatgacgt gcactatacc tacgaatccg
     2821 atgcggaggc gcatgatgtg cttttgtgca tccagaccgg taaaaaggtg tcggacgagg
     2881 ataggatgcg ttatgatggc gggcagtttt tcgtgaagag cgaggagcag atgagagcgc
     2941 ttttcccata tgccacagag gccattgaga atacacagaa aattgccgac agatgcaatg
     3001 taacgcttga gtttggcaat tacaagattc caaagtatga ggtgccagag ggctatgata
     3061 gtgccgaagc cttccttaca gagctttgcg agaagggctt tagggagaag tatataggct
     3121 gtggcgagta tagtgatgag gagcttaaaa agatacatgc agatatggac tatgagcttg
     3181 gtatcataaa gaccatgggc tttatagagt atatccttat cgtgtgggat tatatcaact
     3241 ggtgccgcac gcacgactgc tgggtaggcc cgggacgagg ctcagcggcg ggaagcaggg
     3301 tgtgctattg taccggaatc acggatattg acccggttaa atacaatctg ctctttgaaa
     3361 gatttcttaa ccctgagcga gtttccatgc ctgatataga tgtggatttc gagtatgcgg
     3421 agagatatcg cgccatagag tatgttcagg agaagtacgg acatgattcg gtaacgcaga
     3481 tagtcacctt tggtacgatg gcggcaagag gtgtcataaa atcagtcgga aaagcgctgg
     3541 atttcccata tgcagagact gacaggcttg caaagatggt gcctcaggag ctgaatatca
     3601 caattgacaa ggcacttcag atgaaccctg aattcaaggg catttacgag agtgatgcaa
     3661 gagtgcatga aatgatagat atggcaaaaa ggctcgaggg actgccaaac catacttcag
     3721 tgcatgccgc aggagtggta atttatccgg gtgtggcgag cgattatgtg cctcttggaa
     3781 gggcaaatga cggaagccct accgcagagt acaatatggt acagctcgag gagcttggcc
     3841 ttttaaagat ggattttcta gggcttcgta cacttacggt gctaaaggat tccgtgaaga
     3901 atatcaaggc atcaaggggc attgatgtgg atatcgacca tattgatttc aacgataagg
     3961 gcacacttga ctttatcgga acaggaaaga cggaaggggt attccagctc gagtctgccg
     4021 gtatgcaaag cttcatgaag gagctcagtc cacagagctt tgaggacatc gtggcaggta
     4081 tttcgctcta ccgtccgggt cctatggatt tcatcccaaa ctatataaag ggcaagaaca
     4141 atccaaatga gataagctat gtgacaccgg agcttgagag tattttagag ccgacctacg
     4201 gttgtatagt ataccaggag caggttatgc agatagtgca gaagctcgcg ggctatacaa
     4261 tgggacaggc ggacaacatc cgccgtgcca tgagcaagaa gaagcagtat gtcatcgacg
     4321 cagagcgcca gagctttgtg tacggagata aggagcgtgg cataaagggc tgtgtggcaa
     4381 acggcataga tgaaaaagcc gcaaacagca tatacgattc gatggttgat ttcgctaagt
     4441 atgcgttcaa caaatcgcat gctgccgcat atgcgaaaat cagtgttcag actgcatggc
     4501 tcaaaaacta ctatccggtc gaatttatgg cagcgctcat gacatctgtt atagacaatt
     4561 cagccaaggc ggcgcagtat ctttacagct gtcgtgatat gggtattaag gtgcttccac
     4621 cggatattaa ctgtggacag ggcgagttta ccgcagaggg cgaccatgtc agatatggta
     4681 tgtatgccat caaatcactc ggcaggccgg tcatagacca gataatcgag gagagagaaa
     4741 gtggcggcag attccgcaca ctctctgatt ttattgagag aacctgccag aaggagctta
     4801 tcaacaagcg tgcgattgag aatctgataa aggctggtgc ctgtgattca ctcgacggca
     4861 cgagaaagca gatggcaatg gtgtatgcat cgattgtgga cagggtgcag catgacaaaa
     4921 agtcaactat ggcagggcag atgagtctgt ttgatctggt ctcagacgag gacaggcagt
     4981 cgtttgagct taagtaccca aatgttggtg aatatgatgc agaggaggct cttgcatatg
     5041 aaaaagaggt gcttggcatc tatttaagcg gacatcctct ggaaaaatat gcagctgctc
     5101 taaagaaaaa catcacagca aatgcgatag attttgagct cgaagaggag agcggtgccg
     5161 ttaagataaa ggacaatgcg cacgtggtaa tcggaggcat gatagcaaca aagactatta
     5221 agtttaccag gtacaatcag gctatggctt ttatcacgat agaggatctg acaggaacag
     5281 tcgaggttat tatttttcca agagattacg agcgttatca gagatattta caggatgatg
     5341 ctaaaatatt tgtcatcgga catgcgacgg tcgaggatga gcagaacggc aagataatat
     5401 gcgagagaat cataccgttt gatgagcttc cgaaacaact ctggcttaag tttgatacga
     5461 tggatgacta taagaaaaat gagagcaggc ttcttgagac aatccgtgaa tcagacggag
     5521 cagatgaggt cataatttac atcgcagata caaagcagat gaagaaactt ggcagaaatt
     5581 attccgtaaa tgcaaataat gaactcatgg agcagctttt ggaattttta gacaaagaaa
     5641 atgcgaaaat tgttgaaaag aatattgaaa aattatgcta aaacaagtac aatagattgg
     5701 gattagaata aatgataatt ttttaacata tggaggttat accaatggca aaagagatta
     5761 acacaatagg tgtacttaca agtggtggtg acgcaccggg aatgaacgca gcaattcgtg
     5821 ccgttgttcg tcaggctctc tcaaagggca agaaggttaa aggaatcaag agaggatacg
     5881 caggtctgtt aaatgaagag atcgtggata tggattcaaa gagcgtttca gacacaatct
     5941 cacgcggtgg tacaatctta cagacagcca gatgtaagga gttcgttaca gcagagggac
     6001 agcagaaggg tgctgagatc tgcaagaagc atggtatcga tgctatcgta gttatcggtg
     6061 gtgacggatc cttccagggt gcgcagaagc ttgctgcact tggcatcaat acaatcggtc
     6121 ttcctggcac aatcgacctt gatatcgcat gtactgatta cacaatcggt tttgatacag
     6181 cctgcaacac agctatggag gctatcgaca aggtacgtga tacatctaca tcacacgaga
     6241 gatgctcaat catcgaggtt atgggacgtg gagccggata cattgcatta tggtgcggtc
     6301 ttgcaaacgg cgctgaggaa gtattacttc ctgagaagta tgacaacaac cttgaggcac
     6361 tcatcgagag agtagctcac gcaagagagc tcggtaagca gcattacatc atcatcaatg
     6421 ctgagggtgt aggacattca caggagatgg ctaaggaaat cgaggctgca acaggcatcg
     6481 agacacgtgc aactatcctt ggacatatgc aaagaggtgg aagcccatca tgcaaggatc
     6541 gtgtatacgc tacaatcatg ggcgctatgg cagttgattt actctgcgag ggcaagtcaa
     6601 acagagttat tgcccacaag gatggcaatt tcgtagattt cgatatcgac gaggcacttg
     6661 ctatgaagaa gagtgtagac gagtacatgt acgatatctc taaatctctg gtttagcatc
     6721 taatatagtt acgataatat gaatgccctt gtatccggca cattaatgca ggtaattgcg
     6781 tgcatttgaa gtgccggtgc aggggcattt tgaatgtcag tagaattata tgattaaagg
     6841 gtcaaaaggt atcattataa tatacattgg atatagaaag agaagaaaat gattacaagt
     6901 gtatcaaaca aaagcataaa aaatgtgcag gctcttttaa gcaaggtaaa ggagcgtaaa
     6961 aaacagggcg tattcgtggt tgagggtcca aagatgagct ttgaggcgcc atatgactgg
     7021 gtaagtgctg tatatatgtc tgagtcattt tttaatgatg acaggtttaa ggaagagaag
     7081 aagcgtttcc ttgacaaaac agaaatagtg agtgactcgg tgtttaagag catgtctgac
     7141 acgcagactc cgcagggcgt gcttgctgta gtaaagatgc cgcactatga tatagatgaa
     7201 ctgtttaagg gggataagac agagcttctt gtgcttgaga gcatacagga tccgggaaac
     7261 ttaggcacta tggtccgtac cggtgagggc gcaggtgtaa gcggcattat aatgaatcgc
     7321 acaacagtgg acatttttaa tccaaagact gtgcgctcaa ccatgggctc actctaccgt
     7381 gtgccgtttt acatcacgga tgaccttccg gagactatag agtatgcaaa gtcaaaggga
     7441 gtttatctct acgcagctca tttaaagggt aagtgctctt atgacagacc ggactacacc
     7501 ggagcctgtg gctttatgat aggaaatgag ggcaatggat tatcagatga gatagctgac
     7561 atggcggaca catatatccg catccctatg gagggagctg tggagtcgct taatgccgca
     7621 atatcagcca ctttgctcat gtatgagtgc aaccgtcaga gaagactttt gggagaataa
     7681 tatgcgtatt tggtttaaga tttatactga cacgcatctg atgcgtgata ttacaataga
     7741 aaatgatagt gacgacacac gtacacacaa gatttttaag tctcttgagg atgcctgcct
     7801 tgcatttgac ctgggaaagc ccatctggct tgacagcaac gtaagtgatt tcaagaggca
     7861 tggaaagaca cgctttacga aggacaattt tgtggaggat atcgactttg attttctcga
     7921 gatgcacatc atcgaggagg attgatacct cattatggat atgatgagtt attattgata
     7981 taaggacggt ccgcacactg caggcacaga gcggcacaca agtactctct cgttcgctac
     8041 gctacgccat taagaatctg taggaattat gccttttgtt ttctatgctt gacgaaaccg
     8101 gcttttatgc gccgtccatg gcgcataacg ccttgccccg gcgtccatgc cggtgcattt
     8161 ctggttgtga agcataattc aacagattct aaatagcttc gctatgcgct tacgagagag
     8221 tacttgtgtg ccgctctgtg cctgcagcgt gcggacctgt gcacataaaa ataagcaaca
     8281 tataaaataa caagaataaa aaatatataa ataataggta taatttgcca attatgcgaa
     8341 taaatatgct ataatcgatt aaaagagtct gataaatata caggcgaata gatcacaggg
     8401 aggtttttaa tggaacaaga gtttgcggag ggcattgata gctgggagac tatgatgttt
     8461 ctatacaatt ctgcactgaa agaggtcaat acgaagctcg agatactaaa tgacgagttc
     8521 gttcacatac ataattataa tccgatagag tacataaagt cgcgtatcaa gactcctgaa
     8581 agcatagtta agaagctcaa aaagggcggc tacgagagca ctatagagaa tatggtcaca
     8641 tatgtaaagg acatagcggg catcagaatc gtgtgctcgt tcacatctga tatatacagg
     8701 cttgctgaga tgattggcag gcagaatgat ttgaccgtca tatcggtaaa ggactatatg
     8761 aagcatccga aggcgagcgg ctacaagagc taccacatga ttgtttcggt gcctattttt
     8821 ctttcaaaca aggtggttga cacaaaggtt gagatacaga ttcgtacaat agctatggat
     8881 ttctgggcaa gccttgagca caagatatat tacaagttcg agggcaatgc tccggagtat
     8941 atcagccgcg atctgcgcga gtgtgcaggt attgtaggac agcttgatgc aaagatgctt
     9001 tcgctcaatg aggcaatcct tgaggccaag gagaagcagg agcaggagag ggaggagaaa
     9061 atacgagaag atgaacatca ttaatgttga aaatataaca aagtcatata cgggaaggaa
     9121 gcttttttct aaagcttcct tttatgtgca ggaaaacgaa aaaatcggta tcataggtat
     9181 aaatggtaca ggaaagtcta cacttttaaa gattgtggct ggtgatgaag agccggatga
     9241 ggg
//
LOCUS       DPUF01000076            2928 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_30183, whole
            genome shotgun sequence.
ACCESSION   DPUF01000076 DPUF01000000
VERSION     DPUF01000076.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_30183"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            9..>546
                     /locus_tag="DHV81_02785"
     CDS             9..>546
                     /locus_tag="DHV81_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCI93444.1"
                     /translation="MSDSTCDLSQEVLERYDIDIIPLHIVKGDEELEDGPQIDIHALY
                     EWADKNKTTPKTSAPSIETAMNVMRPYIDEGREIICFSISSEMSTSINVIRMAAEELD
                     AEDKVTVIDSRNLSTGIGLLVVEAAVMAADGKSREEIKAGIDELIPKVRASFVVDTLV
                     YLYRGGRCNAVSALIGGAL"
     assembly_gap    547..665
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            962..1405
                     /locus_tag="DHV81_02790"
     CDS             962..1405
                     /locus_tag="DHV81_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCI93445.1"
                     /translation="MLMPSIFGENLFNDDWMDFSFPDVDKALYGKHAKNVMKTDVKET
                     DNSYEVDIDLPGFKKDEIEAKLENGYLTISAAKGLDKEEKEEKDGKYIRKERYSGAMS
                     RSFYVGDELKQEDIKAKYEDGILKLSVPKKEQKKVETTKHIAIEG"
     gene            1610..>2928
                     /locus_tag="DHV81_02795"
     CDS             1610..>2928
                     /locus_tag="DHV81_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI93446.1"
                     /translation="MSKDEYLITDAPLKALTVFAMPMILGSFFQQVYNMADSIIVGQF
                     VGSSALAAVGACAALTNVFICIALGAGVGAGVLVSRYFGARDYGKMKTIVSTSLISFL
                     VLSVLLGVFGFFFSHSMMSLLQTPADILDEAVLYLRVYFVGFPFLFMYNILSTMFTSI
                     GESRIPLGLLIFSSVLNIFMDLWMVAGLDLGVFGAALATLIAQGISAVFSLLIFLSRM
                     RRYKSRFEWFDRHELRSMLRIAVPSVLQQSTVSIGMMIVQAVVNPFGTQALAGYSATM
                     RVENVFSLIFVSIGNAVSPFVSQNLGAKKTERIKKGYHAALVLDLCFAVLAFIVIETL
                     HTQISSLFLGKDGTALAYQVSGDYMRWLGFFFIFMGIKMATDGVLRGLGIMRPFLAAN
                     MVNLAIRLSVALICAPRFGIAFVWLAVPAGWLANFLISYVALRKSWPA"
BASE COUNT          805 a          484 c          697 g          823 t
ORIGIN      
        1 taaaaattat atcagacagt acatgtgatc tgtcacagga agttttagaa agatacgata
       61 tagatattat tccgcttcat attgtaaaag gagatgaaga actggaggac ggaccgcaga
      121 tagacattca cgcattgtat gagtgggctg ataaaaataa gactacacca aagacttcag
      181 ctccatctat agaaacagca atgaatgtaa tgcggccata tattgatgaa ggcagggaga
      241 taatatgctt ttccatatca agtgagatgt cgacatcaat caacgtgatc agaatggcgg
      301 cagaagaact tgatgcagaa gataaagtta cggtgataga ttcaagaaat ctgtccacag
      361 gaataggcct gcttgttgtt gaggcggcag ttatggcagc agatgggaaa tcccgtgagg
      421 agataaaagc cggaattgat gaattgatcc cgaaggtaag agcaagcttt gttgtagata
      481 cacttgtgta cctttacaga ggtggcagat gcaatgcggt ttctgcgctt atcggaggag
      541 cattggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnataga gacaagagca ggtggtgtga tttcatcaca ttgtgggcct ggcacactcg
      721 gagtgttatt tattgcaaaa taatggagtt tcatatagta agtgctttga agctgctcgg
      781 aaaatataaa cattttataa attattagca ctcactattg acgagtgcta ataataggtg
      841 tataacatag tcaagaacaa aggaaaggaa caaaaagcaa aggctttgag ttcttgaaac
      901 atcccggttc caaagaaaca ggttgacaca gattttatag attaagaagg agagatgact
      961 tatgttgatg cctagtattt ttggagaaaa cttatttaac gatgattgga tggatttttc
     1021 atttcctgat gttgacaagg cgctttatgg taaacacgca aagaacgtta tgaagactga
     1081 tgtcaaggaa acagacaata gctacgaagt agatatagat cttccgggat tcaagaagga
     1141 tgagattgag gctaagctgg agaatggtta cttaactatc agcgcagcta aaggacttga
     1201 caaggaagag aaggaagaaa aggatggcaa gtatatccgc aaggagcgct attccggtgc
     1261 tatgagcagg tctttctatg taggtgatga gctgaagcag gaagatatca aggcaaagta
     1321 tgaggacggt attcttaaac tttcagttcc gaagaaggag caaaagaaag ttgagaccac
     1381 aaagcatatt gctattgaag gataaactta atttaaatca tgttatgaga agtcgcacag
     1441 gcatatggcc tgggcggctt tttaaattaa aatacaaagg agcagtatga tgtttgcatt
     1501 agagggaaag cataagaaat ggaaaaatgt cgataaagaa tgctatattg taatttgtct
     1561 ttcaatcgga tatactatag cttaattata gcggaatgag ggaaaaataa tgtcaaaaga
     1621 tgaatactta attacagatg ccccgttaaa ggcgttgaca gtttttgcaa tgcccatgat
     1681 tttgggcagc ttttttcaac aagtatataa tatggctgat tcaatcattg ttggtcagtt
     1741 tgtaggctcc tctgcacttg cggcagttgg agcctgtgct gcgcttacga atgtgttcat
     1801 ctgtatagca ctgggagccg gtgtaggagc cggtgtactt gtgagccgct atttcggcgc
     1861 cagggattac ggcaaaatga aaacaatagt atcaacatca ctgattagct ttctggtttt
     1921 aagcgtactt ctgggtgtct ttggcttttt cttttcacat tcaatgatga gcttgctaca
     1981 gacacctgca gatatactgg atgaggcagt gctgtatctt agggtttatt ttgtgggatt
     2041 tccgtttctg tttatgtaca acatactttc aacgatgttt acatcgattg gcgagtcaag
     2101 gattccgttg gggcttttga tattttcctc tgtgttaaat atatttatgg acctttggat
     2161 ggtggcaggc cttgacctgg gtgtgttcgg tgctgccctt gcaactctaa ttgctcaggg
     2221 aatctctgca gtgttttcac ttttaatttt ccttagccgt atgcgccggt ataaaagtcg
     2281 ctttgaatgg tttgacagac atgagcttcg ttccatgctt cggattgctg tgccttcggt
     2341 tctccagcag tctacggtgt caattggcat gatgatagta caggcagttg tgaatccgtt
     2401 cggtacacag gcgcttgcag gatattcggc aacgatgagg gtcgagaatg tattttcact
     2461 gatatttgta tccattggaa atgcagtttc tccgtttgtt tcccagaatc tcggagcaaa
     2521 gaagacagaa cgtatcaaaa aaggctatca cgcagcgctg gtgttagacc tgtgttttgc
     2581 agtgcttgct tttatagtaa ttgaaacact gcatacacag atttcctcat tattcctggg
     2641 caaagacgga acggccctgg catatcaggt atcaggggat tatatgagat ggcttggctt
     2701 ctttttcatt tttatgggta taaagatggc caccgatggt gtgcttcgtg gacttggcat
     2761 catgcgtccg tttctcgctg caaatatggt aaacctggca atccgcctct cggttgcctt
     2821 aatctgcgcg ccgcgtttcg gtattgcatt tgtctggctt gctgtaccgg ccggctggct
     2881 tgccaatttc ctgatttcct atgtggctct cagaaaatcc tggccggc
//
LOCUS       DPUF01000077           13489 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4953, whole
            genome shotgun sequence.
ACCESSION   DPUF01000077 DPUF01000000
VERSION     DPUF01000077.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13489)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4953"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="DHV81_02800"
     CDS             complement(<1..635)
                     /locus_tag="DHV81_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI93447.1"
                     /translation="MIETAIIYIKRNKIKSLIIILIIATIILGEIIGLLLSNASEKSE
                     RDAYLYNGAALVIEDDNGGFTKSDYDEIRKVKYVTGLGAWRELIADPVDTENVKEHKG
                     IDPENKDVRNRADKMVIVANMDVQQYSLFSWEKNVSLIEGEFPTYENKGIIVEQRYAK
                     KNNLKVGDEVKYTIGDQPEVTSLQICGIYKVDSDFEIYDSNEEGTSVYIHSP"
     gene            complement(651..1061)
                     /locus_tag="DHV81_02805"
     CDS             complement(651..1061)
                     /locus_tag="DHV81_02805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93448.1"
                     /translation="MEKNRKQIIICAAIVACVCVISVLITYNILQQKNHLTVELYYGT
                     FDFSEYQNVKSTSKLAIIHDSDEKQGEYEMEIENTDKVETGIWKWKDDGYITLYQDDK
                     AVANLVYMNGKYLFLDADVEIQKLKKISETAIVK"
     gene            complement(1400..2029)
                     /locus_tag="DHV81_02810"
     CDS             complement(1400..2029)
                     /locus_tag="DHV81_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93449.1"
                     /translation="MNIELDNVSKNFKNQTVLENVNLKLESGKIYGLVGRNGSGKSVL
                     LKIMTGLMEPSDGIIKYGGIPLKKGQYAKDVGIVLDCIGFMPEYTAKENLLLLAKINK
                     KTGEERINEILKQVGLEPDSKKVYRKFSLGMKQKLAIAQAFMEEPKVLLLDEPMNALD
                     EQSVQDMRIMFREYVNKKPAIMVITSHHKEDIEILCDQVYSIKNGYVNL"
     gene            complement(2026..3414)
                     /locus_tag="DHV81_02815"
     CDS             complement(2026..3414)
                     /locus_tag="DHV81_02815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93450.1"
                     /translation="MKSRWFQLLITIFIIFSYMQFEYVRYCMECDAGYQEYSYANAVI
                     NIVGDRYLILLVSGILIVWSIAGLLAVTRNNFYLKLRIGSEHRANVIEVVYAFKYAVI
                     IAVIQWVVINMIAVVNNFYNVKSQMLHVTNVYQLFGNLLLFYWLLGCITILFNKLINT
                     GWAANICSIVCLLINVVISNRISFKDSFVGRKSWIAHLMVTDVKTYSFYIVYWFVSIA
                     FVIMVIFYRAVILNCFKRLIAICVKSYYGLTFMAAVVIWSIYGFVSKQYMSGITDYFA
                     GFKKLDIFLMLYLFYQMPIWILLYYFLTKNFSFYYIQFALRKGNTISWMKHLAIIFLV
                     CTYLYYGVGMMVIAFISGTVINVNLIMEMSNMILQTIALLLLVFLCWLHEAEDKHIGF
                     IIIIIYHIVAGTLFSWLPLTQGLYISHMTSQPECMVFQMAECLVIGTGVAVILKNKQE
                     YIILRKWKAENI"
     gene            complement(3404..4171)
                     /locus_tag="DHV81_02820"
     CDS             complement(3404..4171)
                     /locus_tag="DHV81_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93451.1"
                     /translation="MKTEEENMLYIKKYSGFIISIVIGLLLYEEANYAYTYRTATAYS
                     DHMIYRLILSMGSGSSGWNGILFPILAALPMGISYVREYRSGYLKLRLQKMSRKRYVA
                     VTLIQNSILGGLGLLIPYVVYCLHLYFQYGTKAYLNIKEGETVIMFHPELAASNTIAY
                     VCLLGTFVLLAGVTFATFAIGISAWIKNTFLTLILPFVLCIAMAILIPDNRFDLLLIF
                     TPNGYAWASVPIILIMSGILILTGIILFAVGVKNNEK"
     gene            complement(4291..5481)
                     /locus_tag="DHV81_02825"
     CDS             complement(4291..5481)
                     /locus_tag="DHV81_02825"
                     /inference="COORDINATES: protein motif:HMM:PF00512.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93452.1"
                     /translation="MKSDFKAVKAVQIIFGAIILSCCIFGGIYGIGVNGLTIFFNNSR
                     YIYDHESPYVQEFQQYVSQNNIATSDISECDEWMDYHCVLFCIIEKDGEAVYSKLNVD
                     KFIVSAKNMYDSRKIAKYQLPVNFADGEANVYIYAGYTEKYYLGVIILGIVISILSGA
                     YVIYRCVNNIIKDYKVNIGKAHEQERKARDEKDQLMRNMAHDLRTPLTGLMTYIDILK
                     LQNKSNESINKNLDILTSKVNELRDLSNLLLDFSIASSDENIHLDEPSFVEYAIGDYL
                     SEMHTIISQNGFCVNIDGIYWEKVKVAVNGSLLSRIFSNLTNNICKYADIDEQVVMET
                     VYSKNIFEICISNTVCKEKSLLESTGLGLKNVELLMRRMHGRAIYETKEYCFYVKLRF
                     PITN"
     gene            complement(5471..6166)
                     /locus_tag="DHV81_02830"
     CDS             complement(5471..6166)
                     /locus_tag="DHV81_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93453.1"
                     /translation="MYKILIVEDDETIRNGIKTLLELQGYYVDIAVNGVDGLKKFDET
                     YSLVIMDIIMPLLSGIDACKKLREESSVPILFLTAKSSEIDIMQGFEVGGDDYLVKPF
                     SNMELISRVKALLRRRNVYDREIIDDNAGNEYIDRKDIRIYENKNRVEVNGIGVKFTR
                     KEYEIIKLLAKHPDRIYTLEVIYSNIWEELYTYNSGNTVMVHIKNIRNKFYEICNRAV
                     IETIWGKGYKFEK"
     gene            complement(6214..7632)
                     /locus_tag="DHV81_02835"
     CDS             complement(6214..7632)
                     /locus_tag="DHV81_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCI93454.1"
                     /translation="MFIGREKELATLNGLYNSDKFEFAVIYGRRRVGKTALISHFIQD
                     KKAIYFMGVESNEKQNLENFSQAIMEYASGIMPGTSFVSFQAAIEYVFKLCENERLVL
                     VIDEYPYVAKASKSMASTLQMLIDKNKDNSKMMLILCGSSMSYMEDNVLAYKAPLYGR
                     RTAQMKILPFDFEDSCKYFNGFSAEDMALIYGIVGGTPQYLLQMNDSMSVEDNIKNVF
                     LNPASAIFEEPENLLKQEVREPAVYNAIITAVATGSSRMSEIATKIGETTSVCSQYMK
                     NLINLGLIRKETPYGEKESRKSIYAIADNMFRFWYRFIPDNNSIISRGATELAYKRIA
                     PNLSDYMGRIFEEICMQYLWKLLLDGEAPVEFSNLGRWWGTDPQERIQTEIDIMGDQD
                     KSTALFGECKWINENVDVKVLEKLKKRSRLFRYENVHLFLFAKKGFTQGCIEEAGKMG
                     NVSLVTYDQIYEYLNDRGITAF"
     gene            complement(7888..9610)
                     /gene="ptsP"
                     /locus_tag="DHV81_02840"
                     /pseudo
     CDS             complement(7888..9610)
                     /gene="ptsP"
                     /locus_tag="DHV81_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741270.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     assembly_gap    8215..8294
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9641..9904)
                     /locus_tag="DHV81_02845"
     CDS             complement(9641..9904)
                     /locus_tag="DHV81_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCI93455.1"
                     /translation="MVSKTMVVKAAEGLHMRPAGVIAREAGKFDSDVTIVFGDKRINA
                     KSLVNIIAACIKKDAEITFECEGADEEAALAKMEELEADNFGD"
     gene            complement(10111..12132)
                     /locus_tag="DHV81_02850"
     CDS             complement(10111..12132)
                     /locus_tag="DHV81_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCI93456.1"
                     /translation="MLSEKGLLIIEKLAEHNNELVTSKALAASTGMSERSVKTYLKEV
                     ADFCEQNSMTLDRKPGKGMKPCFSDAQIGKILDVAGRKSAAVSQKKRQNYISYILLSG
                     WDTYTYALFSEELNVSKNVIMDDINELDAELLLFGIKVHRTAGYGIYATGSELDIRKA
                     MRHFCRYPISDKQVIKTDDHRLSRRAAEVIANNFRSVNLSMAVDMIHHVERRFDIIFT
                     DYTFQMLAEYIAIALFRVDVEKELKPDELDLSNRMRDDANDGDAGTETEQQVQKNGFV
                     MTEHENMAKEAAGFLDRHHGISLSQPEIMYLAMLFSCAEGQNRVVMSCEEALSIEDEM
                     IVYLSNLLAANLIENELLRESMRSFLPGSIARTHFGIEIDNPFLSDITQSYASLFTVC
                     FTVSRYYEKYTGAMPSEDEIAFIALQVGGALHRNPMTVRAVLIGAAGYATGSIIAGKI
                     ENRVPDVRIVSILSSDRIEHIDEYDCDLILSTIDTQADIHKDMRFLYVSPLISAQDEK
                     NIRNKCFELMTGQSAEVSEFSQMLSEEFIIFEKKAKNRKDVLKRACQLLINKGIVQSE
                     FARDVLEREKVEATAVGCNIAIPHGKPEHVNRCQILVIRLDKPIEWGERMADMIFLLA
                     INFDSVNTTKAFFHDFTKLLNENGATDRLREAASSHELCAAIRKELGWN"
     gene            complement(12151..12855)
                     /locus_tag="DHV81_02855"
     CDS             complement(12151..12855)
                     /locus_tag="DHV81_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCI93457.1"
                     /translation="MISKTEFEKTHIAKGEEENRKKAVVFDLDGTLLYTLEDLKNATN
                     YALKQNGMPERTLDEVRRFVGNGVKLLMERAVPDGADNPKFEKTFSDFKEYYEAHCND
                     NTAPYDGIMELLKELKLNGVKLAIVSNKLDPAVKELNQLYFKEYMTSAVGEMEEEGIR
                     KKPAPDMVQKALKELGVTQDEAIYVGDSDVDIATAKNSGLECVSVTWGFRDVEFLKEH
                     GATNLIDEPVELLNYV"
BASE COUNT         3695 a         3165 c         1995 g         4554 t
ORIGIN      
        1 ggactatgta tgtataccga ggtcccctct tcatttgagt catatatttc gaagtcactg
       61 tccactttat aaattccgca tatttgtaaa gacgttacct ccggttggtc tcctatagta
      121 tatttaactt catcccctac cttaaggtta ttttttttcg catatcgctg ttctacaata
      181 attcctttat tttcatatgt tggaaattcc ccttcaataa gagatacatt tttttcccat
      241 gaaaacaggc tatattgctg cacatccata tttgcaacaa tcaccatttt gtcagcccga
      301 ttgcgcacat ctttattctc cggatctatt cctttatgtt cctttacatt ctctgtatcc
      361 accggatctg caatcagctc tctccacgcc cccagtcctg ttacatattt tacttttctg
      421 atttcgtcat aatcactctt tgtaaagcct ccgttatcat cctcaattac taatgcggct
      481 ccattatata aatatgcatc tctctcagat ttttctgagg cattgcttaa tagcagacca
      541 ataatttcac ccaggattat tgtcgcgata atcagtatga taatcaaaga ttttatttta
      601 tttcttttta tatatataat agctgtttct atcatatccc atctcctcaa ttattttact
      661 attgccgttt cagatatctt tttaagtttt tggatttcaa catcggcatc caagaaaaga
      721 tatttaccat tcatatacac tagatttgca actgctttat catcctgata caaggtaata
      781 taaccatcat ctttccattt ccagattccc gtttcaacct tatcagtatt ctctatctcc
      841 atttcatatt caccctgttt ttcatcgcta tcatgtataa tggctaattt actggtactc
      901 ttaacatttt gatactctga aaaatcaaaa gttccataat agagttccac tgtcagatga
      961 tttttttgtt gtaatatgtt atatgtaatt aatacactga ttacacagac acaagcgaca
     1021 atcgctgcgc agataataat ctgttttcta tttttttcca ctacatactt cccccttctt
     1081 tattgttcaa tcaatgccgg ctttattcta accgtttgat acttcttgct ataaacgata
     1141 tataatacca cttttagtga ataaaatcct catttatgtg aagttttcaa ggtacaaatt
     1201 tatggttata aggtatcttt aacagaatac cttttgaccc tcaaatcata tatataatag
     1261 ctgtttctat catatcccat cttctcaata ctacctgaca ttaaaaaaag ccatagacat
     1321 ctatggcttt acaatactat atacatataa agaattagct gaaaaatcat ttaagaatta
     1381 taaataattc taaagaaacc taaagattca catacccatt ttttatacta taaacctggt
     1441 cacacagaat ttctatgtct tccttatggt gacttgttat aaccataata gcaggttttt
     1501 tattcacata ttccctaaac attattctca tatcctgaac agactgttca tccagtgcgt
     1561 tcattggctc atccaagagc aataccttag gctcttccat aaaagcctga gctattgcca
     1621 gcttctgctt catacctaaa gagaacttac gataaacctt tttggaatcc ggctcaaggc
     1681 ccacctgttt gagaatttca ttgattcttt cttctcctgt tttcttatta atttttgcca
     1741 gtaaaagtaa attttccttt gcagtatact ccggcatgaa gcctatgcag tccagcacta
     1801 ttccaacatc ctttgcatac tgtccttttt tcagagggat gcctccgtat tttattattc
     1861 catccgacgg ctccattaac ccagtcatta tcttcaataa aacacttttt ccactaccat
     1921 ttcttccaac gagaccatat attttcccgg attccaattt taaatttaca ttttccaaca
     1981 cggtctgatt tttaaaattc tttgatacat tatccagctc tatattcata tattttctgc
     2041 cttccactta cgtaaaatta tatactcttg tttatttttt aatattacag caacgcccgt
     2101 tcctataaca agacactctg ccatttgaaa taccatacac tcaggctgag atgtcatatg
     2161 cgaaatatac agcccctgtg taagcggcag ccacgaaaat aatgttcctg ccacaatatg
     2221 gtatataata attattataa atccaatatg tttatcttcg gcttcatgta accagcatag
     2281 aaataccagc agtaacaagg ctattgtctg taaaatcata ttactcattt ccattatcaa
     2341 atttacattt attaccgttc ccgaaataaa tgctattacc atcattccca ctccgtaata
     2401 cagataagta cataccagaa atattattgc cagatgcttc atccagctaa ttgtatttcc
     2461 ttttctcaag gcaaactgaa tatagtaaaa cgaaaagttc tttgtcaaaa aataatacaa
     2521 taaaatccag attggcattt gataaaacaa atatagcatt aaaaaaatat caagcttttt
     2581 aaatccggca aaatagtctg taatgcctga catatattgt tttgaaacaa atccgtatat
     2641 gctccatata acaactgcag ccataaacgt aagaccataa tatgacttaa cacatattgc
     2701 aatcagtctt ttaaaacagt ttagtataac ggccctataa aatataacca ttattacaaa
     2761 cgcaatactt acaaaccaat aaacaatata aaaactatat gttttcacat ctgtaaccat
     2821 caaatgtgca atccatgatt ttcttccaac gaaagaatct ttaaagctta tcctattact
     2881 gataacaaca tttataagca ggcacacaat actgcaaata ttagctgccc atcctgtatt
     2941 tatcaatttg ttaaataaaa tggtaataca accaagcaac cagtaaaata acaataagtt
     3001 tccaaatagc tgatacacgt ttgtcacatg aagcatctgt gacttaacat tataaaaatt
     3061 atttacaaca gcaatcatat taattaccac ccactggata actgcaatga taaccgcgta
     3121 tttgaatgca tacactactt caatgacatt tgccctatgt tcacttccaa tccgcaattt
     3181 aagataaaaa ttattccgtg taaccgccaa taatccggca atgctccata caattaaaat
     3241 cccgctgaca agcaatatta aatatctgtc tccaacgata tttataactg cattggcata
     3301 tgaatactcc tgatatccgg catcacattc catacaataa cgaacatatt caaattgcat
     3361 atagctaaaa ataatgaata tagttattaa taactgaaac catctacttt tcattatttt
     3421 ttactcctac cgcaaacaaa atgatgcctg ttaaaatcaa tatacctgac ataattaata
     3481 ttatcggaac agatgcccat gcatatccat taggcgtaaa tattaaaagc agatcaaatc
     3541 tgttatccgg tatcaatatt gccatggcta tgcaaagaac aaatggcagt atcagcgtca
     3601 gaaaagtgtt tttaatccat gccgatatgc cgattgcaaa tgtagcaaat gtaactcctg
     3661 ccaataaaac aaacgtacca agtaaacaaa catatgctat agtattcgag gctgccagtt
     3721 caggatggaa cataataact gtctctccct cttttatatt caagtaagcc ttagttccat
     3781 actgaaaata taaatgcaga caatatacaa catacggaat aagcaaaccc aagccgccaa
     3841 gtattgagtt ttgtattaat gttacagcaa catacctttt tcttgacatt ttttgcaggc
     3901 gcagcttcaa atatccgctt ctatattctc tgacatatga tattcccata ggaagtgctg
     3961 ccagtatcgg aaagaggatt ccattccagc cactgcttcc ggaccccatg ctaagtatca
     4021 atctgtatat catatgatca gaataagcag ttgcagttct atatgtatat gcgtaatttg
     4081 cttcttcata aaggagcagt cctataacaa tggaaataat aaaccccgaa tactttttta
     4141 tatatagcat attttcctct tccgttttca tttatttatt aatcgttcct atagatatct
     4201 acaaaaaaat cgtatcacaa tattttaaag aaaaagaaag gaacttctga aattttctaa
     4261 aaatttctaa agatttctga agtttgtagt tcagttcgta atcggaaatc gcaattttac
     4321 ataaaagcag tattcttttg tttcgtaaat tgctctgcca tgcatacgtc tcatcaacag
     4381 ctcaacattt tttagtccta atcctgtact ctccaataag cttttttcct tacacaccgt
     4441 gttgcttatg catatttcaa atatattttt tgaatatact gtttccatga ccacttgctc
     4501 atcaatatca gcatacttgc aaatattatt agtaaggtta ctaaatattc ttgatagtaa
     4561 acttccattt acagcaactt taaccttttc ccaataaatg ccatcaatat taacacaaaa
     4621 accattctgg cttataatgg tatgcatttc agaaagataa tctcctattg catattctac
     4681 gaaagaaggc tcgtccaaat gtatattctc gtctgaactt gcaatagaaa aatccagtaa
     4741 cagatttgaa agatctctta attcattaac tttagatgtc agaatatcca aatttttgtt
     4801 aatgctctca tttgatttgt tttgcaattt taagatatca atgtatgtca tcaatcccgt
     4861 taatggtgtt cttaaatcgt gagccatatt tctcatcaat tgatcttttt catcccgtgc
     4921 tttccgttcc tgctcatgtg ccttaccaat atttacttta taatctttta tgatattatt
     4981 aacacatcta tagattacat aagctccgct taagattgat attacaatgc ctaaaattat
     5041 gactcctaaa taatactttt cagtatatcc ggcatatata tatacattag cctctccatc
     5101 agcaaaattt accggtagtt gatatttagc aatttttcta ctatcataca tgtttttcgc
     5161 tgatacaata aatttatcta cattcaattt actatataca gcttctccat ctttttctat
     5221 aatgcaaaac aaaacacaat gataatccat ccattcatca cattccgata tatctgatgt
     5281 ggctatatta ttctgagaca catactgttg aaattcctga acatatggag attcatggtc
     5341 ataaatatat cttgaattat taaaaaaaat agtaagacca tttaccccta tgccatatat
     5401 ccccccaaat atacagcacg ataatattat tgcaccaaat ataatttgaa ctgcctttac
     5461 tgctttaaaa tcacttttca aatttgtatc cctttcccca tatagtttct atgacagccc
     5521 ggttgcatat ttcatagaat ttattcctta tattttttat gtgtaccata actgtatttc
     5581 ccgaattata tgtatacagt tcttcccaaa tatttgaata aatcacctca agtgtgtata
     5641 ttctatcagg atgttttgca agcaatttga ttatttcata ctcttttcgt gtaaatttaa
     5701 cacctatgcc attcacttct actctattct tattttcgta tatccttata tcttttctgt
     5761 ctatatattc atttcctgca ttgtcatcaa ttatttctct atcatataca tttctacgcc
     5821 ttaataatgc cttgacacgc gatataagct ccatattgga aaacggcttc acaagataat
     5881 catcaccgcc cacttcaaag ccttgcatta tatctatttc tgatgatttt gcagtaagaa
     5941 ataaaattgg gacacttgat tcttccctca attttttgca ggcatctata cctgataaca
     6001 acggcattat tatatccata ataaccaggc tatatgtttc atcgaatttc tttaaaccgt
     6061 ccacaccatt tacagcaata tcgacataat atccctgtaa ttctaataat gtctttattc
     6121 cattacgaat tgtttcatca tcctcgacta ttaatatctt gtacatctta tatcctcaac
     6181 tttgattaaa ttcagtaaaa aatccatata tacttaaaaa gcagttatcc ctctatcatt
     6241 taaatattca tatatctgat catatgtcac cagcgataca tttcccattt ttccggcttc
     6301 ctcaatgcac ccttgggtaa atcctttttt tgcgaataaa aataaatgga cattctcata
     6361 tctaaataat ctgcttctct ttttcagctt ttcgagcact tttacatcca cattttcatt
     6421 tatccactta cattctccaa acaatgcagt gcttttatcc tgatcaccca ttatatcaat
     6481 ttctgtctgt attctctctt gtggatccgt tccccaccat cgtccaagat tgctaaattc
     6541 cacaggtgct tccccatcaa gcagcaattt ccacagatac tgcatacata tttcttcaaa
     6601 tatcctgccc atataatctg acagatttgg agcaattctt ttatacgcaa gttctgttgc
     6661 acctctgcta atgatagaat tattatcagg tataaaccta taccagaatc taaacatatt
     6721 atcagctatt gcatatattg attttctgga ctccttttcg ccatatggag tttctttccg
     6781 tattaagcca agattaatca gatttttcat atactgtgag catacacttg tcgtttcgcc
     6841 tatcttggtt gcgatttccg acatacggga gctgcctgtt gctactgctg ttattattgc
     6901 attatataca gcaggttcac ggacttcctg ctttaacaga ttttcaggtt cctcgaagat
     6961 tgctgatgcg gggttgagga acacattttt tatattatcc tctacactca tgctgtcatt
     7021 catctgtaaa agatattgcg gagtaccacc aacaatacca tatatcagag ccatatcctc
     7081 tgctgaaaat ccgttgaaat atttgcagct atcctcaaaa tcaaacggaa gtattttcat
     7141 ctgggcagtt cttctgccat acaaaggtgc cttatacgcc agcacattat cttccatgta
     7201 agacatagat gagccacata atataagcat cattttagaa ttatctttat ttttatcaat
     7261 aagcatctgc aaggttgaag ccatgctttt cgaagctttt gccacatatg gatactcgtc
     7321 tatgacaagc accagtcttt cattttcaca taatttaaat acatattcta ttgcagcctg
     7381 aaatgacacg aatgaagtcc ccggcattat tcctgaagca tattccatga ttgcctgact
     7441 aaagttctcc agattctgct tttcgttgct ctctactccc ataaaatata ttgctttttt
     7501 atcctgtata aaatggctta tgagagctgt cttccccact cttcgtctgc cgtagattac
     7561 tgcgaattca aatttatccg aattatataa tccgtttaaa gttgcaagct ctttttctct
     7621 tcctataaac atagctcttc tccctccatt tctatacaca atattttatt acttatatat
     7681 gatttagtca atcataattt agttaatcgt aaataaccaa atcgtaaatg actaaatatt
     7741 catctttcat ttcgcgtaca aaaaaagacg gtcgtcccgg tccccaccgg ttcagccgtc
     7801 tttataataa atttcaatgt tatactttta tattttactc ttatttatac ttttttacta
     7861 acttttatac ttgtttggat ttagcaccta ctgctgtgca agcactgcct gtacctctgc
     7921 ctctgtagcg catgccatta ccttgtctgc caatgccttg cattctgcca tgctgctgtc
     7981 tgcgatggtc tttctggtct tgagcacact tgtggcactc actgagaact catcaagtcc
     8041 gaatgcgagt aacaacggaa tgagctttgg atctgctgct gcctctccac acatgcctac
     8101 catgattccc tctttcttac cgcactcgat gatacgcttg attgcgcgaa gcactgccgg
     8161 attgaaagct gaataaaggt atgctacctt tgagtttcca cggtctactg ccatnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnacatct gccatcatgc aggctgccgg tgtctccatc atgataccga
     8341 tttcgaggtt cttgttgtat gcaatgccct ctgcatcaag ctctttcatg atgtcagcta
     8401 ccattgcctt tacactgcga agctcatcta cgcctgtcac aagcggaacc attatcttta
     8461 tcctgccgaa tgcactggct ctcacaagtg cacgaagctg tgtattgtag atatctgttc
     8521 tctggagaca gaatctgata gctctgtaac ctaagaatgg gttgtcctct ttctcaaggc
     8581 ccagatatgg gatttccttg tcgcctccga tatcaagtgt acggatgatg acaggcttgc
     8641 ccttcattgt ctctgctact gatttgtatg cctcaaactg ctcttcctct gtaggaattg
     8701 tatctctgtc catgaaaagg aactctgtac ggaaaagtcc tattccctct ccgtcacact
     8761 ctacagcctt ctttgcgtcc tcaggctttc cgatattgca tacaagctcc acctttgtgc
     8821 cgtctgctga tactgtcggc acacccgcaa atttctttaa gagctctttc tcttttttgt
     8881 actcctcaag cagcccagcg aatttctgct ctgtctcagc gtcagggttt acgattgcct
     8941 ctccgcttgt tccgttgagt accactctgt caccattttt gacagatgta cagattccgt
     9001 caatactgag aactgccggg atttccatag ctcttgcaag tattgctgag tgggaggttc
     9061 tgccgcccac ctctgtgagt attcctgcga tattctctgc cacgatacct gctgtcatgg
     9121 atggtgtcag gtcatgtgcc acgagcactg tgcctgccgg agctgctgag atatcatagt
     9181 cctcgattcc aagcagaatc ttaaggattc tggctttgat atccctgaaa tctgttgctc
     9241 tctgctgcaa aagctcatca ggtatctgtg caaatatctc tgctgtctgc tcaagcacct
     9301 ggtcgagcgc cttctctgcc gtgattttct cacttactat gagcgctgta atctgctcct
     9361 ctatggtcgg atcctgaagc atctggatat gtccgcgaag gatatccgca tcctttgctc
     9421 cgactgtctt ttccattctg tctgccatgg cgtttgtatc atttacaaat ttctcaatgg
     9481 ctccctggag acgtgcgatt tcagcgtctg tatctgtaat tttcttattc tcaatcacta
     9541 tctcatgctc ttcgataatg accacatttc cgattccgat tccttctgag ccggcgattc
     9601 ctttgtacat atcacacctc cgattgactt tgctggttga ttaatctccg aagttgtctg
     9661 cctcgagctc ttccatcttt gcaagtgctg cttcctcgtc agctccctca cactcgaatg
     9721 taatctctgc atccttcttg atgcatgctg cgatgatgtt tacaaggctc tttgcgttga
     9781 ttctcttgtc gccgaatacg attgttacat ctgaatcaaa cttgcctgcc tctcttgcga
     9841 taactcctgc aggtctcatg tgaagtccct ctgctgcttt tactaccatt gtcttgctta
     9901 ccatagtttt gtcctcctga atttaaaatt attggtttgt ttatttcgct cacacatatg
     9961 attaactgta tcgctctctg ctattcatca gcctgtcata tgtccgttgc tttctatggt
    10021 tttattgtac tgtgtggtat gagttttttc aatattcccc aaaatgcaaa attgcaccat
    10081 ctctggtgca attttgtatt aatgtgatat tcagttccac cccagttctt tccggatagc
    10141 tgcacaaagc tcatgagatg aagcagcctc cctgagcctg tccgtcgctc cattttcatt
    10201 cagaagcttt gtgaagtcat ggaaaaatgc ctttgtcgtg ttgacactgt caaagtttat
    10261 cgcaagcaga aatatcatgt ctgccattct ctcgccccac tctatcggct tgtcgagacg
    10321 tataacaagt atctggcagc ggtttacatg ctcaggcttt ccgtgcggta tggctatatt
    10381 gcagcccaca gcggttgcct ccaccttctc acgctccagt acatccctcg caaactcgct
    10441 ctgcactatt cctttgttta tgagaagctg gcacgcgcgc tttaaaacat ccttgcggtt
    10501 tttcgccttt ttctcaaata tgataaattc ctccgacagc atctgtgaaa actcgctgac
    10561 ctccgcgctc tggccggtca ttagctcaaa gcacttgttt ctgatatttt tctcatcctg
    10621 cgcactgatt agtggagaaa catacaaaaa tctcatatcc ttgtggatat ctgcctgtgt
    10681 gtctatggtc gagagtatga ggtcgcagtc atactcgtca atatgctcta tgcggtctga
    10741 cgacaaaatg cttactatcc ttacatccgg caccctgttt tctattttac ccgctattat
    10801 actgccggtc gcatagcctg ctgcaccgat taacactgcg cgcactgtca taggatttct
    10861 gtgcagggct ccgcccacct gcaaggcaat aaaagctatc tcatcctcgc tcggcatggc
    10921 gcctgtgtac ttttcgtagt atctcgatac tgtaaagcat accgtaaaga gactggcata
    10981 cgattgtgtg atatccgaaa gaaacggatt gtctatctct atgccgaaat gagtcctcgc
    11041 aatggagccc ggtaaaaagc tacgcatgct ctcacgtaga agctcatttt caatcagatt
    11101 ggctgcgaga agatttgaca gatacactat catctcatcc tctatgctaa gtgcctcctc
    11161 acaggacatg acaacacggt tttgtccctc ggcacaggaa aaaagcattg ccagatacat
    11221 gatttcaggc tgtgagaggc tgatgccgtg atgtctgtcc agaaagcctg ctgcctcctt
    11281 tgccatgttc tcatgctctg tcattacgaa tccatttttc tgtacctgct gctctgtttc
    11341 ggtgcccgca tccccatcat ttgcatcatc acgcattcgg tttgacaaat ctaactcatc
    11401 cggcttcagc tccttctcca catccaccct gaaaagtgca atggctatgt actctgccag
    11461 catctgaaat gtgtaatccg taaagatgat atcaaagcgt ctctccacat gatgtatcat
    11521 atcaactgcc atcgacagat ttacgcttct gaaattattg gctatcacct cggccgcgcg
    11581 tcttgaaagc ctgtggtcat cggtcttgat aacctgctta tcactgattg gatatctgca
    11641 gaaatgcctc atggcttttc tgatatcaag ctcactgcct gtcgcatata ttccgtagcc
    11701 cgccgttctg tgcaccttaa tgccaaagag cagaagctct gcgtcaagct catttatatc
    11761 atccatgatg acatttttgc tgacatttaa ctcctctgaa aaaagcgcat acgtatatgt
    11821 atcccagccg cttagcagaa tatacgatat gtagttctgt cgcttcttct gcgatactgc
    11881 tgccgatttc cgccctgcca catccagaat ctttcctatc tgcgcatcgc taaagcacgg
    11941 cttcatgcct ttgcccggct ttctgtcgag cgtcatagag ttttgctcac aaaaatcagc
    12001 cacctccttc agatatgttt tcacacttcg ctctgacata cctgtggacg cagcaagagc
    12061 ctttgatgtg acaagctcat tgttgtgctc tgccagcttt tcaattatca atagtccctt
    12121 ttctgaaagc ataaatcaaa gttctcctga ttatacgtaa tttaacaatt cgaccggttc
    12181 atctatcaga tttgttgcac catgctcctt caaaaactct acatccctga agccccatgt
    12241 gacagatacg cactccagac ctgagttttt cgctgtggcg atatccacat cggaatcccc
    12301 cacatagatt gcctcatcct gtgtcactcc aagctccttt agtgccttct gcaccatatc
    12361 aggtgccggc tttttgcgga taccctcttc ctccatctca ccgactgccg atgtcatata
    12421 ctccttgaaa taaagctgat tgagctcctt taccgccggg tcaagcttgt ttgaaacaat
    12481 agcaagcttt acgccgttta atttaagctc ctttaaaagc tccatgattc catcataagg
    12541 tgctgtattg tcattgcaat gagcctcata atattccttg aaatccgaaa atgtcttttc
    12601 aaactttgga ttgtctgctc cgtccggcac tgcgcgctcc atcagaagct ttacaccgtt
    12661 tcccacaaat ctgcgcacct cgtcgagtgt acgctccggc atgccatttt gtttcaatgc
    12721 atagtttgtt gcatttttta aatcctcaag tgtatagaga agtgttccat caaggtcgaa
    12781 aacgaccgct tttttacgat tttcctcctc accctttgct atatgtgtct tttcaaattc
    12841 tgtcttgctt atcatatatg cctcattcct atcatcattt ggcagaacca taatatctcc
    12901 aggtctgcct gatatataac tgctgtcatc cttattctta aatagcttta ccttgtgctt
    12961 tagcacaaat gcccttgaaa aatccgcctc aacaggtatg cacactctgg catgtgccat
    13021 tccatccact atagtctccg tatccttatt cttgaacttc gggacataat atgtctgttt
    13081 cacagtaaac cgccagtcct tgtatatcct gaagcttttg ttcagcctgt ccagatttaa
    13141 aatccgtaca ctgccgtctt ctcctattat gaccaccgta tcttcaccaa ccggtgtgga
    13201 gatatccccc ttagctgtcc tcactcccat gcagcagccc acaggcagta tatcctttgc
    13261 aatgatatat cccatcggta tcgggcgttt tttataatcc acttttttga agccactcgt
    13321 atcaaaatct tctccgtaaa ttatctttgt atcgtttaaa taatcaaaaa tcctctcttc
    13381 caaaaaagat ttgattgcag aatccgaagg acgcttgtgt gtatcatcgc tgctgtactg
    13441 aaatcttggt gtcacgtctt tttctttgca gtagacatta tagctgtct
//
LOCUS       DPUF01000078            3678 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8474, whole
            genome shotgun sequence.
ACCESSION   DPUF01000078 DPUF01000000
VERSION     DPUF01000078.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8474"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2333)
                     /locus_tag="DHV81_02860"
     CDS             complement(<1..2333)
                     /locus_tag="DHV81_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCI93458.1"
                     /translation="MRDNTFSKKVFIKTVSDNLKNKYRKTIDDATQQELYQAVSEAVK
                     DVIMDEWIATQRVMDKDDPKVVYYMSMEFLMGRALGNNLINLSAYKEVKEALNEIGVD
                     INAIEDQEPDPALGNGGLGRLAACFMDSLATLGYPAYGCGIRYRYGMFKQQISDGFQI
                     EVPDNWLKDGYPFELRRPEYCYEVKFGGYVQESTDENGELHFEQKDYQSVLAVPYDMP
                     IVGYDNNVVNSLMIWDAEPKNGFSLESFDQGDYDKAVEQENLARNLVEVLYPNDNHVK
                     GKELRLKQQYFFVSASIQRALARFKKHHSDLKDLPNKVVFQMNDTHPTVAVAELMRIL
                     VDEEHLSWDDAWDITTRCVAYTNHTIMAEALEKWPIEIFQRLLPRVYQIVDEINRRFV
                     MQINERYPGNEDKVRKMAILYDGQVKMANMAIVAGYSVNGVAKLHTEILKNQELHDFY
                     EMFPEKFNNKTNGITQRRFLAHANPLLADWATKKVGAGWITDLTMLSKLKAYADSPVA
                     QQEFAEIKRANKVRLAKYIKEHNGIDVNPDSIFDVQVKRLHEYKRQLMNILHVMYLYN
                     KIKENPSMDFYPRTFIFGAKAAAGYRNAKKTIKLINSVADVINNDASIGGKLKVVFIE
                     DYKVSNAEIIFAAADVSEQISTASKEASGTGNMKFMLNGALTIGTMDGANVEMFDEVG
                     ADNMFIFGMSSDEVISHENRHDYNPVDVYNSDAELRKVVNQLVDGTYSPNDHELFREL
                     YNSLLNPQQGQVADRYFILADFRSYANAQQKINDYYK"
     gene            complement(2392..>3678)
                     /locus_tag="DHV81_02865"
     CDS             complement(2392..>3678)
                     /locus_tag="DHV81_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCI93459.1"
                     /translation="DAIRWYFYTASAPWIPKKFSGKLVLEGQRKFMGTLWNTYAFFVL
                     YANIDNFDATKYSLEYDKLAVMDKWILSKLNSAVAGVDECLSNYKIPEAAKYLQEFVD
                     DLSNWYVRRSRERFWAKGMEQDKINAYMTLYTALVTISKAAAPMIPFMTEDIYRNLVC
                     SIDKSAPESIHLCDYPVVDEKLIDKKLEADMDEVLKVVVLGRACRNEANIKNRQPIGK
                     MFVKAPEKLDEFYVDIIADELNVKEVEFTDDVSHYTTYKFKPQLRTVGPKYGKYLGQI
                     KAVLAELDGHKAMEELNSTGSLKLDSISDEVVLSKEDLLIEMTQMEGYVTQSDNNITV
                     VLDTNLTPELIEEGFVREIISKIQTMRKDAGFEVMDHIAVTYEADEKLSNVFEKFGDE
                     IKTEVLAVSIKSGELKGYEKDWKINGEAVKLAVEKQ"
BASE COUNT          923 a          903 c          711 g         1141 t
ORIGIN      
        1 ttcttgtaat aatcattaat cttctgctgt gcatttgcat atgagcggaa gtctgcgagg
       61 ataaagtagc ggtcagccac ctgtccctgc tgtggattga gcagtgagtt gtaaagctcc
      121 ctgaaaagct catggtcatt tggcgaatat gtgccgtcta cgagctggtt tactaccttt
      181 ctgagctctg catcgctatt atatacatca acaggattgt agtcatgtct gttctcatgt
      241 gagatgacct catcagagct cattccaaag ataaacatgt tgtcagctcc tacctcatcg
      301 aacatctcga cattggcacc atccatagtt ccgatagtga gcgctccatt gagcatgaac
      361 ttcatgtttc ctgttccgga tgcctccttg ctcgctgtgg aaatctgctc gctgacatct
      421 gctgccgcaa aaataatctc ggcattggat accttgtaat cctcaataaa tacgaccttg
      481 agcttgccgc cgatagaagc atcgttattt atgacatcag ccacactgtt gatgagcttg
      541 attgttttct ttgcatttct gtagccagct gctgcctttg ctccaaaaat aaatgttcta
      601 ggatagaaat ccatagacgg attttcctta atcttattgt acagatacat cacatgaagg
      661 atattcatga gctgtctctt gtactcgtga agtctcttaa cctgcacatc aaagatagaa
      721 tccggattta catcaatacc attgtgctcc ttgatatact ttgctaaacg caccttgttt
      781 gctctcttaa tctcagcaaa ctcctgctgt gcaacagggc tgtccgcata agccttgagc
      841 tttgaaagca ttgtaagatc tgtaatccag ccggctccta ccttttttgt tgcccagtct
      901 gcaaggagtg gatttgcatg tgctaagaat ctacgctgtg tgattccgtt tgtcttgtta
      961 ttgaacttct cagggaacat ctcatagaaa tcatgaagct cctgattctt aagtatctct
     1021 gtgtgaagct ttgcaacacc gtttacagaa tagcctgcaa cgatagccat atttgccatc
     1081 ttgacctgtc cgtcatagag gatagccatc tttcttacct tgtcctcatt gccaggatat
     1141 ctctcattaa tctgcatcac aaatcttctg tttatctcgt caacaatctg gtatacacga
     1201 ggaagcagtc tctggaatat ctcaattggc cacttctcaa gcgcctcagc catgattgta
     1261 tggttagtgt aggctacaca tcttgtagtg atatcccatg catcatccca tgacagatgt
     1321 tcctcgtcca caaggattct cataagctct gcgactgcaa ctgtagggtg agtatcgttc
     1381 atctggaaaa cgaccttatt cggaagatct tttaaatcgc tgtgatgctt tttaaacctt
     1441 gcaagtgcac gctggatgct tgctgataca aagaaatact gctgctttaa gcgaagctcc
     1501 ttgcccttta catggttatc gtttggatag agcacctcaa caaggtttct tgcaagattt
     1561 tcctgctcca ctgccttgtc gtagtcaccc tggtcaaagg actcgagcga gaagccgttt
     1621 tttggctctg catcccatat catgagagaa ttgaccacat tgttgtcata accgactata
     1681 ggcatatcat aaggcactgc aagcaccgac tgatagtcct tctgctcgaa atgaagctct
     1741 ccgttttcat ctgtgctctc ctgcacatat cctccaaact tgacctcata gcagtactcc
     1801 ggacgacgaa gctcgaatgg atatccgtcc ttgagccagt tgtcaggcac ctcaatctgg
     1861 aagccgtctg aaatctgctg cttgaacata ccatatctgt atctgattcc acagccgtat
     1921 gcaggatatc caagtgtggc aagtgaatcc atgaaacacg ctgccagtct tccgagtcca
     1981 ccgtttccaa gtgccggatc aggctcctgg tcctcaatag cattgatatc cacaccaatc
     2041 tcattgagag cctccttgac ctccttgtat gctgagaggt ttataaggtt gttgccgaga
     2101 gctcttccca tcaaaaactc catggacata tagtaaacaa cctttggatc gtccttgtcc
     2161 atcactctct gtgtggcaat ccactcatcc atgatgacat cctttacggc ctctgataca
     2221 gcctgataaa gttcctgctg tgtggcatca tctatagtct tacgatattt attttttaaa
     2281 ttgtctgata cagtcttaat aaacaccttt ttgctaaacg tattatctct catcatattc
     2341 ctccttatgg cactaaaagg gagcaaatta atgctccctc tcgtgcagag attattgttt
     2401 ttcaactgca agcttaacag cctcaccatt aatcttccaa tccttttcat agcccttaag
     2461 ttcgcctgat ttaattgata cggcaagaac ctcggtcttg atttcatcac caaacttttc
     2521 aaatacattt gaaagcttct catctgcctc gtatgtcaca gcgatgtggt ccataacctc
     2581 aaagcctgcg tccttacgca ttgtctggat cttactgatg atttctctta cgaaaccttc
     2641 ctcgataagc tcaggagtga ggttggtgtc aagaacaacg gttatattgt tgtcagactg
     2701 tgtcacatag ccctccatct gagtcatctc gataagtaaa tcctctttag ataatacaac
     2761 ttcatcgcta atgctgtcaa gcttgagaga gccggtgctg ttaagctctt ccatggcctt
     2821 atgaccatca agctcagcta aaactgcctt aatctggcca aggtatttac catacttagg
     2881 acccactgtt ctaagctgtg gcttgaattt gtaggtggtg tagtgtgata catcatcagt
     2941 gaactcaacc tcttttacgt tgagctcgtc tgcgatgata tccacataaa attcatccag
     3001 tttttccgga gcctttacaa acatttttcc gattggctga cggtttttga tatttgcctc
     3061 gttacgacat gcacgtccga gtactacaac ctttagtacc tcatccatat cagcttcgag
     3121 ctttttgtca atcagcttct catcaactac aggatagtcg cacagatgaa ttgactccgg
     3181 tgcgctcttg tcgatagagc agacaagatt tctgtagata tcctctgtca tgaaaggaat
     3241 cataggagct gctgcctttg atatggtgac aagtgctgtg taaagtgtca tgtatgcgtt
     3301 tatcttgtcc tgctccattc cctttgccca gaaacgctca cggctgcggc gtacatacca
     3361 gttactcaaa tcatctacga actcctgaag atactttgca gcctccggaa tcttgtagtt
     3421 tgacaggcac tcatccactc ctgccactgc tgagttgagc tttgaaagaa tccacttatc
     3481 cattactgca agcttatcat actccaggct gtattttgta gcgtcaaaat tatcgatatt
     3541 tgcataaagc acgaagaatg cgtatgtgtt ccagagtgtt cccataaact tacgctgtcc
     3601 ctcaagcacg agctttcctg aaaacttctt tggaatccat ggagctgatg ctgtgtagaa
     3661 ataccatctg atggcatc
//
LOCUS       DPUF01000079            4051 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6464, whole
            genome shotgun sequence.
ACCESSION   DPUF01000079 DPUF01000000
VERSION     DPUF01000079.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6464"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="DHV81_02870"
     CDS             complement(<1..128)
                     /locus_tag="DHV81_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCI93460.1"
                     /translation="MEREQDFDNEIEIDLKDLFLEIISYWQWIILVTIATGAVAFA"
     gene            complement(153..854)
                     /locus_tag="DHV81_02875"
     CDS             complement(153..854)
                     /locus_tag="DHV81_02875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCI93461.1"
                     /translation="MIDIHCHLLYGVDDGSKSLEESVEMLKIAKKQGITGIILTPHLR
                     HGMFKHPLEKIERHYKKLMPYANKLGIELKLGTEYHVATDMIDAFHGGLCHTLADTQY
                     ILTEYSHSSEYSFVYKMTREAIFAGYIPVIAHVERYECLSDISRIEDLQELGALIQVN
                     ADSVLGIDGRHFKRYTKKLLKAGLVDVIASDSHGTKERVCNMKKCYEYVAKKFGDDYA
                     QEIMQTTPENIINGR"
     gene            complement(1017..1197)
                     /locus_tag="DHV81_02880"
                     /pseudo
     CDS             complement(1017..1197)
                     /locus_tag="DHV81_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743883.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    1095..1138
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1584..2048)
                     /locus_tag="DHV81_02885"
     CDS             complement(1584..2048)
                     /locus_tag="DHV81_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCI93462.1"
                     /translation="MNVIEGKMTQDGIKIGIVVARFNEFITSKLLGGAKDGLVRHDVP
                     EENIDVAWVPGAFEIPLIAKKMAKSGKYDAVICLGAVIRGATSHYDYVCNEVSKGIAS
                     VSLESEIPVMFGVVTTENIEQAIERAGTKAGNKGYDCALGAIEMINLVRQIG"
     gene            complement(2171..3367)
                     /locus_tag="DHV81_02890"
     CDS             complement(2171..3367)
                     /locus_tag="DHV81_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HCI93463.1"
                     /translation="MKFNTVEEALDELRAGKIILVTDDEDRENEGDFICAAEFATTEN
                     INFMAMHGKGLICMPMSQEYVKKLRFPQMVQENTDNHETAFTVSIDHVSTTTGISAAE
                     RSITAMKCVSDDAKPEDFRRPGHMFPLLAKKNGVLERNGHTEATVDLCRLAGLKECGL
                     CCEIMREDGTMMRTTELMELAKKWDIKFITIAALQEYRKIHEKLVDCVTEVKLPTKYG
                     DFKAYGFVNRLNGEHHVALVKGEIADGRDVLCRVHSECLTGDVFGSQRCDCGQQLEAA
                     MRQIDEEGRGILLYMRQEGRGIGLINKLKAYELQEQGMDTLEANLALGFAGDEREYYI
                     GAQILRELGVHSLRLLTNNPDKVYQLSEFGMEISERVPIQMSATAHDLFYLKTKQNRM
                     GHILSY"
     gene            complement(3479..>4051)
                     /gene="ribE"
                     /locus_tag="DHV81_02895"
     CDS             complement(3479..>4051)
                     /gene="ribE"
                     /locus_tag="DHV81_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCI93464.1"
                     /translation="VTRLMPHAFTADVMHETINRSSLANAMRGAHVNLERAMAADGRF
                     GGHIVSGHVDGTGRIVEVKKDDNAIWYTIQAAPQLMRYIVEKGSVTIDGISLTVAKVA
                     EDNFSISAIPHTVSQTVLKDRKVGAVVNLETDIIGKYVEKLLFKGQSDGITEEMTGTQ
                     TIKENVPHKSGENKKQPEGITRDFLAKYGF"
BASE COUNT          856 a         1001 c          791 g         1359 t
ORIGIN      
        1 atcgcaaatg ccactgcacc tgtcgctatc gtaacaagaa ttatccactg ccaatatgaa
       61 attatctcaa gaaacagatc ctttaagtct atctctatct cgttgtcaaa atcctgttct
      121 ctctccatta cttatccccg tatttaaact tattatcttc cgtttattat attttccggt
      181 gtggtctgca tgatttcctg tgcatagtca tcaccgaact tctttgccac gtactcatag
      241 cattttttca tgttgcagac gcgctccttt gtgccatggc tgtcgcttgc tattacatca
      301 acaagccctg ccttcaaaag cttctttgta tatctcttga agtgtctgcc atctatgccg
      361 agcactgagt ctgcattgac ctgtatgagc gctccaagct cctgcaaatc ctctatacgg
      421 cttatatcac tgagacattc atatctttcc acatgtgcta tgaccggtat atatcctgca
      481 aatattgctt cgcgcgtcat cttgtataca aacgagtatt cgctggaatg tgaatactct
      541 gtgagtatat actgtgtatc tgccagtgta tggcacagcc caccatgaaa ggcatctatc
      601 atatcggttg ccacatgata ctctgtgccg agctttaatt ctattccaag cttatttgca
      661 tatggcatca gctttttata gtgtcgctct atcttttcaa gcggatgctt aaacattcca
      721 tggcgcaggt gcggtgtgag gattatgcct gtgatgccct gctttttggc tatcttgagc
      781 atctctaccg attcctcgag cgactttgag ccgtcgtcca ctccgtataa taaatggcaa
      841 tgtatatcta tcaaaattca tggtcctccg ttttgttttc ctgaactatc attatactat
      901 atacagattt ttcgtgcaat tctttagata ttaaattttt cttctttttt gttgggaggg
      961 cgtggcgtaa gcgccgggca tccgtgcagc gcacacgcac tcccgcgcct gctacgctac
     1021 gctgctaaga gactgtacga attatgcctt ggggtggttc tatgcttgac gaaaccggct
     1081 tttatgcgcc gtccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     1141 gcgcataacg ccttgcccgg ccgtccatgg ccgggcattt ctgtttttct tgggcataat
     1201 tctacagtct ctatgcagct tcgctattcg caggcgtggg agtgcgtgtg cgctgccgga
     1261 tgcccggcgc ttacgccact tacgttgtga cagtatttta cgattttaga tttgcggttt
     1321 tttagttatt ggttggttgc aagcttgaca acacgctgtc aagcttgcgt ttgaaagtta
     1381 ttttttggtg gattactacg atatctcatg atgacaatta acatatgtgt cagttttcag
     1441 atatatcaca tataataagt attcgaggtc caaatatcaa agatatttga acctcagagg
     1501 attatgctat aatactataa tgccatagca cagtagcact atagcgccat aatgttataa
     1561 tgccattatg ctatataatt atattatccg atttgtctga cgagatttat catttcgatc
     1621 gcaccgagtg cacagtcata tcctttgttt ccggcttttg ttccagctct ttctattgcc
     1681 tgctcgatgt tttcagtggt gactactcca aacattaccg ggatttctga ttcgagtgat
     1741 actgatgcga ttccctttga tacctcgttg cacacataat catagtggct tgttgcgcct
     1801 ctgattactg ctccgaggca gatgactgca tcgtattttc cactttttgc catcttcttt
     1861 gcgatgagtg gtatctcgaa tgctcccggc acccatgcta cgtctatgtt ttcctccggc
     1921 acatcgtgtc ttacgagtcc gtcctttgct cctcctaaaa gctttgatgt gataaattca
     1981 ttgaatcttg ctactacgat tcctatcttg attccatcct gtgtcatttt tccttcgatt
     2041 acgttcattt tttgttctcc ttatgttttt tatttttact cagatgagtt tatctgcaat
     2101 tattaactat ttagaatttg ttactattca gaatccccgc acacattgca aaaacgctcg
     2161 atgcggtgta ctaatatgag agtatatgtc ccatgcggtt ctgcttggtt ttcagataaa
     2221 acaggtcatg cgctgtggcg ctcatctgga ttggcacacg ctcgctgatt tccatgccaa
     2281 actctgagag ctggtatacc ttgtcagggt tatttgtgag aaggcgcagg ctgtgtacgc
     2341 caagctctct taatatctgg gctccgatat agtactcgcg ctcatctccg gcaaagccga
     2401 gtgccagatt tgcttcgagt gtgtccattc cctgctcctg aagctcatat gccttcagct
     2461 tatttatcag gccgatgcct ctgccctcct gacgcatata gagcagtatt cctctgccct
     2521 cctcgtctat ctgtctcatg gctgcctcaa gctgctggcc acagtcacag cgctgtgagc
     2581 cgaatacatc tcctgtgaga cactcagagt gtactcggca gagcacatct cttccatctg
     2641 cgatttcgcc ctttacaagt gccacatgat gctctccgtt taatctgttg acgaagccat
     2701 atgccttgaa gtcgccgtat ttggtcggca gctttacctc tgtgacacag tcaacgagct
     2761 tttcatgtat ctttctgtat tcctgaagtg ccgctattgt gatgaacttt atgtcccatt
     2821 tttttgcaag ctccatgagc tctgttgtgc gcatcatggt accgtcctca cgcattatct
     2881 cacagcacag cccacattcc tttagtcctg caagcctgca taaatcgact gttgcctctg
     2941 tgtgtccgtt tctctccagt acgccgtttt tctttgcaag aagcggaaac atgtggcccg
     3001 gacgtctgaa atcctcaggc tttgcgtcat cagagacaca tttcattgct gtgattgagc
     3061 gctctgctgc ggaaatgcct gtggttgttg agacatggtc tattgagact gtaaatgctg
     3121 tctcatgatt gtctgtattt tcctgcacca tctgcggaaa acgaagcttc tttacatatt
     3181 cctgcgacat cggcatgcat atcagaccct tgccgtgcat cgccataaag ttgatgtttt
     3241 ctgttgtggc aaattctgcc gcacagatga aatcgccctc attctctctg tcctcatcat
     3301 ctgtgacaag tattattttt cctgcacgaa gctcatcaag tgcttcttct actgtgttga
     3361 atttcatatt ttatgtcctc gtctttctat tgtgatttta tagctattgc gttacatgat
     3421 ttttaaccct tcatccccac gcacatttgc aaacccgcac gatacccatg cttttatgtt
     3481 agaagccgta ttttgccagg aaatctcttg ttattccttc cggttgtttt ttattttcgc
     3541 cggatttatg tggtacattt tccttaattg tctgtgttcc tgtcatttct tctgttattc
     3601 catccgactg ccctttaaac aacagcttct caacatattt tcctatgata tctgtttcca
     3661 gatttacgac agcgcccact tttctgtcct ttagaactgt ctggcttacc gtgtgcggta
     3721 tcgctgatat cgagaaatta tcctctgcca cctttgcaac tgtgaggctt attccatcga
     3781 ttgtgactga gcccttctcg actatatagc gcatgagctg tggtgcggcc tgtattgtat
     3841 accagatggc attgtcatcc tttttgacct ccacaatcct gcctgttccg tcaacatgtc
     3901 cagacactat gtgcccgcca aatctgccat ctgcggccat cgcacgctcc aaattgacat
     3961 gtgctcctct catcgcattt gcaagcgatg aacggtttat cgtctcgtgc atgacatccg
     4021 ctgtaaacgc atgtggcatg agccttgtga c
//
LOCUS       DPUF01000080            5872 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1666, whole
            genome shotgun sequence.
ACCESSION   DPUF01000080 DPUF01000000
VERSION     DPUF01000080.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1666"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="DHV81_02900"
     CDS             <1..471
                     /locus_tag="DHV81_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HCI93465.1"
                     /translation="IEVFDIKVESIDLPEVTMTVHCSKGTYIRTLCNDIGERLGCHGC
                     MKSLLRVRVAGFDLEHALTLSQIQSKVDEGRFDMVMPVDGVFENLPAVHTASDADKLV
                     RNGNKIPAVLTDYLKHSADSDIRYRVYNHEGIFVGVYSYLDETGEFKPVKIFME"
     gene            489..1418
                     /locus_tag="DHV81_02905"
     CDS             489..1418
                     /locus_tag="DHV81_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCI93466.1"
                     /translation="MEYISKTLDFHVKEPSVISFGKFDGLHRGHEFLMEKQIEQSNLH
                     GYKRIIFTFDIPPKSEVLGVESKVIITNDEKEYVFEKSGIDYLIECPFVPQVMTMEAD
                     AFVRWIVKSLNVKCIIVGDDFRFGHNRAGDHKLLSAMAGELGYDLIVVDKIKDGDRDI
                     SSTYIREEIAAGHIKKANELLGYPFFIKGRVVHGRALGRTIGIPTVNLSVPHDKLLPP
                     KGVYMSRVLVRDNWYLGVTNIGCKPTIEGKNPIGAETYIIDFGQDVYGQDIMVELHKF
                     VRPEMKFDSIEALKSQMSADINYTINYAGSHTF"
     gene            1562..2755
                     /locus_tag="DHV81_02910"
     CDS             1562..2755
                     /locus_tag="DHV81_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCI93467.1"
                     /translation="MKNKLKLGQAAGVVAFAIIIGAVSMSSSSTVDARTSLKENRTVN
                     AGVALGLTEAADATELLTAGATSALTYYGAELTVAQNDSNVVTASAQGTPQTDGASQE
                     SEQANEAAQTPTAAQTCGYTNLGMSVISSGNLNIRQEASTDSEVIGILTNHNACELLE
                     DAGEWYKVTSGKVTGYVNKQYLVTGDEAESIAEQEIKTVATVNTETLNVRAEKSTDAA
                     VLSQVGNSEAFTVNSVADGWVEISVDDSVGYISQDYVTLAQALPTAKTIEQVKYGDGV
                     SDVRASVVSYALQFVGNRYVWGGTSLEKGVDCSGFTMRILGKYGISLPHSSRAQPSYG
                     TKISASEAKPGDLFFYGGGRSISHVAIYIGNGQIVHASNKRDGIKVSNAYYRNPICVA
                     RYLPD"
     gene            2898..3164
                     /locus_tag="DHV81_02915"
     CDS             2898..3164
                     /locus_tag="DHV81_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCI93468.1"
                     /translation="MIAKEKKQAIIAEYGRTPNDTGSPEVQIAILSARIAELTEHFKQ
                     NPKDHHSRRGMLKMIGQRRGLLAYLKEIDIERYRSLIERLGLRK"
     assembly_gap    3451..3619
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3620..5440
                     /locus_tag="DHV81_02920"
     CDS             <3620..5440
                     /locus_tag="DHV81_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCI93469.1"
                     /translation="RVIDRPMRPLFPKDYRNDVTLNNLVMSVDPECRPEIVAMIGTAL
                     AVHISDIPFDGPCAMTQMGLVDGEFIVNPSQKQWDEGDLQLTVASTKEKVIMIEAGAN
                     EIPEDQMIEAIYKCHDINQTVIAFMDQIRDEIGKPKHEYESCAIPEQMFEDIKKIVTP
                     EQMEEAVFTDEKQKREENIRAITEQLEEAFADNEEYLAVLGEAIYQYQKKTVRKMILK
                     DHKRPDGRAINQIRPLAAEVDLIPRVHGSAMFTRGQTQICDVVTLAPLSEIQKIDGLD
                     ANITTKRYMHHYNFPAYSVGETKVSRGPGRREIGHGALAEKALVPVLPSIEEFPYTIR
                     AVSETFESNGSTSMASTCASCMSLMAAGVPIKAMVAGISCGLVTGDTDDDFLVLTDIQ
                     GLEDFFGDMDFKVTGTHKGITAIQMDIKIHGLTRPIVEEAIARTREARLYIMDEVMSK
                     AIAEPRKEVNEWAPKIEQITIDPSKIGDVVGQKGKTINEIIDRTGVKIDITDDGAVSV
                     CGTDKEMIAKAIDMIKIITTDFEQGQILEGTVVSIKEFGAFIEFAPGKEGMVHISKIA
                     KERINRVEDVLTLGDKVKVVCLGKDKMGRISFSMKDVKED"
     gene            5559..>5872
                     /locus_tag="DHV81_02925"
     CDS             5559..>5872
                     /locus_tag="DHV81_02925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HCI93470.1"
                     /translation="MSYCENESYTKLKNKIIMKNAKYSINANLVYKNGYSGKNVNIAV
                     LDTGVFKHKQLDGCIKHFMDFVGGKETCYDDNGHGTHVCGILSAADIGMAPGAGLYVF
                     KVL"
BASE COUNT         1800 a          987 c         1440 g         1476 t
ORIGIN      
        1 atcgaggtgt ttgatatcaa ggttgaaagc attgatttgc ctgaggttac gatgacggtg
       61 cactgttcaa agggcacata tatcaggacg ctgtgcaatg acatcggaga gaggctgggc
      121 tgccatggct gcatgaagtc actgcttaga gtaagggtag caggctttga cttagagcat
      181 gcacttacac tttcacagat tcagtcaaag gtggatgaag gccgctttga tatggtgatg
      241 cctgttgacg gagtattcga aaatctgccg gcagtgcata ctgcttcaga tgctgataag
      301 cttgtcagaa atggaaataa aattccggca gtgcttacgg attatttaaa acacagtgct
      361 gactctgata taagatacag agtatacaat catgagggca tatttgtcgg ggtgtattcg
      421 tatcttgatg agaccggtga gtttaagccg gttaagattt ttatggaata gaatagtata
      481 cagatattat ggaatatata agcaaaactc tggattttca tgtcaaggag ccgtccgtca
      541 tctcgtttgg aaagtttgac gggcttcacc gaggacatga atttctgatg gaaaagcaaa
      601 ttgaacagag caatctgcat ggctacaagc gcatcatatt tacgtttgac attcctccca
      661 aatcggaggt acttggcgtt gagtcaaagg ttataatcac aaatgatgaa aaagagtatg
      721 tgtttgaaaa atcaggtatt gactatctga tagagtgtcc gtttgtgcca caggtgatga
      781 cgatggaggc ggatgcgttt gtcagatgga tagttaaaag tcttaatgtc aagtgtatca
      841 ttgtcggaga tgatttccgt ttcgggcaca acagggcagg agaccacaag ctgctttctg
      901 cgatggcagg agagcttgga tacgatctta ttgtggtgga caagataaag gatggagaca
      961 gggatataag cagtacctat atccgcgagg agattgcggc aggacatatt aagaaggcga
     1021 atgaactgct gggatatccc tttttcataa aaggaagagt agtgcatggc agagcactag
     1081 gcaggactat tggcattccg acggtcaacc tttcagtgcc gcacgataag ctgcttccac
     1141 caaagggagt gtatatgtca agagttcttg tcagggacaa ctggtacctt ggtgtaacca
     1201 atataggatg caagcctact atagagggca aaaatccaat cggtgcagag acatatatca
     1261 tagattttgg gcaggatgtt tacggacagg atatcatggt agagcttcat aagttcgtaa
     1321 gacctgagat gaagtttgat tcaatagaag cgttaaagag ccagatgagt gcagatatca
     1381 attatacaat caattatgca ggcagtcaca cattctagtg tggctgtttt tttgcaaaat
     1441 tacccaaaat aacgaaaaaa tgacaaatat tacggaaata ttacggagaa atattgacat
     1501 tttaacaatt tgtgatatac tcgtaaacat gggattatgg atagcatgaa ctggaggcta
     1561 tatgaaaaat aaattaaaat taggacaggc agcaggcgtt gtagcgtttg ctattattat
     1621 aggtgctgta tctatgagtt caagcagcac agtagatgcc agaacatcat taaaggaaaa
     1681 cagaacagtc aatgctgggg tggctcttgg tctgacagag gcggcagatg ctacggagct
     1741 tctcacagca ggagcgacat ctgctctcac atactacgga gctgagctta ctgttgcgca
     1801 aaatgacagc aatgttgtaa cagcatctgc acagggcaca ccgcagacag atggtgcctc
     1861 acaagagtct gagcaggcaa atgaggcagc gcagacaccg acagccgcac agacctgtgg
     1921 ttatacaaat cttggtatgt cggttataag cagcggaaac ttaaatatca gacaggaagc
     1981 ttcgacagac agcgaggtaa taggaatact taccaatcac aatgcctgtg agcttcttga
     2041 ggatgccgga gaatggtaca aggtcacatc aggcaaggtc acaggatatg taaacaagca
     2101 gtatcttgta acaggagatg aggcagagtc tattgcagaa caggagatta agacggttgc
     2161 cacagtaaat acggaaacac tgaatgtgcg tgcagagaag tcaacagatg ctgcggtgct
     2221 ttcgcaggtt ggaaattcag aggcattcac ggttaattct gtcgctgacg gctgggttga
     2281 gataagtgtg gatgattcgg taggatatat atcacaggat tatgtgacac tggcacaggc
     2341 acttcctaca gcaaagacaa tagagcaggt gaagtatggt gacggtgtat cagatgtaag
     2401 ggcatctgtt gtatcgtatg cgttacagtt tgtcggaaat agatatgtgt ggggtggtac
     2461 aagccttgag aagggagttg actgctcagg cttcacaatg agaattcttg gaaagtatgg
     2521 aatctcatta ccgcattcat caagagcaca gccttcatac ggcacaaaaa tcagtgcatc
     2581 tgaagcaaag ccgggagatt tattcttcta cggaggtgga aggagtatct cacatgtcgc
     2641 aatttatatc ggaaacggac agattgtaca tgccagcaac aagcgtgatg gaatcaaggt
     2701 ttcaaatgca tattacagaa atccgatttg tgtcgcaaga tatttgccgg attaataaaa
     2761 aaacagttta caacaaatgc ttactatgct atactgcata agtgtggttt tgcccatgtt
     2821 cggtagttgg acactccgac ctattgctga gcatcataca gttatcaggg cgcattataa
     2881 tataaggagg atcaacaatg atcgcaaagg aaaagaaaca ggctattatc gcagagtacg
     2941 gtagaacacc aaatgacaca ggttcaccag aggttcagat tgcaattctt tcagcaagaa
     3001 tcgcagagtt aactgagcat ttcaaacaga acccaaagga tcatcactca agaagaggaa
     3061 tgttaaagat gatcggtcag agacgtggat tacttgcata ccttaaggag attgatatcg
     3121 agagatatcg ttcacttatc gagcgtttag gactcagaaa atagtatttt ctttagagcg
     3181 gagtaattat tattccgctc tattattgtt tttattattg ttttttgaaa agcagaagta
     3241 agccgagacc actaaagagt gcatgagcgt acatgtattg tttagtagtt tcggaagagg
     3301 aatacacttc tgcaaaagaa ttttaaggag aattaatatg tacaaaagtt attcaatgga
     3361 acttgccggc aggactctta ctgtagatat cggaagagta gcagctcagg caaacggagc
     3421 tgtttttatc aagtatggtg atacaacagt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnc gtgtaattga ccgtcctatg agacctcttt tcccaaagga
     3661 ttacagaaat gatgttacat taaacaacct tgttatgtca gtggatcctg agtgccgtcc
     3721 tgagatagtt gcaatgattg gtacagctct tgctgttcac atttcagata ttccgtttga
     3781 cggaccatgt gccatgacac agatgggact tgttgatgga gagtttatcg taaacccttc
     3841 acagaagcag tgggatgagg gcgatttaca gctcacagtt gcttctacaa aggaaaaggt
     3901 catcatgatt gaagcaggag ccaatgagat tcctgaggat cagatgattg aggcaatcta
     3961 caagtgccac gatatcaacc agacagttat cgcattcatg gatcagatcc gtgatgagat
     4021 tggcaagcca aagcatgagt atgagagttg tgctattcct gagcagatgt tcgaggatat
     4081 caagaaaatt gttactcctg agcagatgga ggaggcagta ttcacagatg agaagcagaa
     4141 gcgtgaggaa aatataagag ctatcacaga gcagttagag gaagccttcg ctgacaatga
     4201 ggagtacctt gcagtacttg gtgaggctat ttaccagtat cagaagaaga cagtcagaaa
     4261 gatgatttta aaggatcata agcgtccgga cggccgtgca atcaaccaga tcagaccact
     4321 cgcagcagag gttgatttga ttcctagagt tcacggctct gcaatgttta cccgtggaca
     4381 gacacagatt tgtgatgttg taacacttgc tcctttatca gagattcaga aaatcgacgg
     4441 acttgatgca aatatcacaa caaagagata tatgcatcac tataacttcc ctgcatactc
     4501 tgtaggagag acaaaggtat cacgtggacc gggacgtcgt gagattggac acggagcact
     4561 tgctgagaag gcacttgttc ctgtacttcc aagcattgag gagttcccat atactatccg
     4621 tgccgtatct gagacatttg agtcaaacgg ctctacatca atggcttcaa catgtgcatc
     4681 atgtatgtca cttatggctg caggtgttcc gattaaggct atggttgccg gaatctcatg
     4741 cggacttgta acaggcgata cagatgatga tttccttgta cttacagata tccagggtct
     4801 tgaggatttc ttcggagata tggatttcaa ggtaacaggt acacataagg gtatcacagc
     4861 tatccagatg gatatcaaga ttcacggact tacaagacct atcgttgagg aggcaatcgc
     4921 aagaacacgt gaggcacgtc tctacatcat ggatgaggtc atgagcaagg caatcgctga
     4981 gcctagaaaa gaagtaaatg agtgggctcc aaagattgag cagattacaa tcgatccatc
     5041 aaagattggt gatgttgtcg gacagaaggg taagacaatc aacgagatta tcgacagaac
     5101 cggtgtcaag atcgatatca cagatgatgg tgctgtttca gtttgcggaa cagataaaga
     5161 gatgattgca aaggcaatcg acatgattaa gattatcaca actgattttg agcagggaca
     5221 gatccttgag ggaacagttg taagcatcaa ggagttcggt gctttcatcg aatttgctcc
     5281 tggcaaagag ggaatggttc acatttctaa gattgcaaaa gagagaatca acagagtaga
     5341 ggatgtactc acacttggag acaaggtgaa ggttgtttgc cttggcaagg acaagatggg
     5401 aaggatcagc ttctctatga aggatgtaaa agaggattaa ttaatatacg ttaaagtata
     5461 acaataatat ccaagtaaag ggctgcacgc aggtgcagtc cttttaatat atgaaattat
     5521 agtaaggtga tatgatgtaa ggcaaaacag gtgcgtctgt gtcatattgt gaaaacgaat
     5581 catatactaa attaaaaaat aaaataataa tgaaaaatgc gaaatatagt ataaatgcaa
     5641 atctggtata taaaaatgga tatagtggta aaaatgtcaa tatagcagtg cttgatacgg
     5701 gtgtgtttaa acacaaacag cttgatgggt gcataaagca ctttatggat tttgtgggtg
     5761 gaaaggagac atgttatgac gataacgggc atggtacaca tgtgtgcggt atattatcgg
     5821 ctgcagatat aggcatggct ccgggggcag gactatatgt gtttaaggtg ct
//
LOCUS       DPUF01000081            5682 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_38611, whole
            genome shotgun sequence.
ACCESSION   DPUF01000081 DPUF01000000
VERSION     DPUF01000081.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_38611"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(90..701)
                     /locus_tag="DHV81_02930"
     CDS             complement(90..701)
                     /locus_tag="DHV81_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93471.1"
                     /translation="MIMKQFLRKTMKNVAAYVELFLSAVLVFVIVVLIIKLIGQCFFD
                     IWHDKIKLEYYMEYIMSLAIGVEFVKMLCTHKPGTIIEVLLFATARQMIVEHLNVYET
                     LVGIGAIAALFAIRKFLFCSFDEADHMICRGSQTVARANFISGTNIPEEKTKLLRDVI
                     SEKLQDEGKNVSIGACVYYQDCALRVDNVKQGVVTRVEVIKNL"
     assembly_gap    752..808
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(977..1603)
                     /locus_tag="DHV81_02935"
     CDS             complement(977..1603)
                     /locus_tag="DHV81_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCI93472.1"
                     /translation="MVERINGKTILTTPISAEDLKGIKIGDIVYLNGSMTTCRDVAHR
                     RLVEEGRELPVDVRNNAIFHAGPIIRPLENDKFEMVSVGPTTSMRMEKFEYEFVKETG
                     VRVIIGKGGMKENTERACKEFGAIHCVFPAGNAVVAATEVEEIVRAEWRDLGMPETLW
                     NCRVKEFGPLIVSIDTEGNNWFEQQKVEFNKKKDAATEEICKQVGFIK"
     gene            complement(1603..2502)
                     /locus_tag="DHV81_02940"
     CDS             complement(1603..2502)
                     /locus_tag="DHV81_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCI93473.1"
                     /translation="MSNEKQVEQLTTYMANFIAHIAKKLPDDVIAKLTELRDKEDSPL
                     SKTIYDTMFRNQELAVKLNRPSCQDTGVLQFWVKCGTKFPLIDELEGLLKEAVVKATF
                     EAPLRHNSVETFDEYNTGKNVGKGTPTVFWDIVPNSDKCEIYTYMAGGGCTLPGKAMV
                     LMPGEGYEGVTKFVMDVMTTYGLNACPPLLVGVGVATSVETAALLSKKALMRPIGSHN
                     ENERAAKMEKLLEDGINAIGLGPQGMGGKYSVMGVNIENTARHPSTIGVAVNVGCWSH
                     RRGHIVFDKDLNYTITTHSGVEL"
     gene            complement(2575..3840)
                     /locus_tag="DHV81_02945"
     CDS             complement(2575..3840)
                     /locus_tag="DHV81_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCI93474.1"
                     /translation="MSPATITLLFLLFAVVMFVWEKIPLGVTSMIVCVGLVIIGVLDW
                     KTAFSGFIDSNVILFVAMFIVGGALFETGMANKIGGVVTHFAKTERQLIIAIMVIVGL
                     MSGVLSNTGTAAVLIPVVIGIAAKSGYSRSRLLMPLVFAAAMGGNLSLIGAPGNLIAQ
                     SAMGEIGLSFGFFEYAKVGLPILIAGILYFAFIGYKFLPEKQGSTESSYDEQKDFSNV
                     PKWKQMMSLIILILTLLGMIFEDKIGIKLCVIGCVGALALIITGVISEKNALKSIDLK
                     TIFLFGGTLSLAAALQETGAGEEIATAVIGILGKNPSPYVLTFVVFMLCCVLTNFMSN
                     TATTALLVPICVSIATEIGADPSAVLMACVIGGSCAYATPIGMPANTMVITAGGYTFK
                     DYAKAGIPLIVVATIVSMVLLPIFYPFFP"
     gene            complement(4021..4665)
                     /locus_tag="DHV81_02950"
     CDS             complement(4021..4665)
                     /locus_tag="DHV81_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI93475.1"
                     /translation="MIGLKPIKIPSAKERVASELRKAILSKQLKEGENLTLESVAQQL
                     NVSITPVREAFQILAHDGLIKLRPNKGAIVLGITETYIREHYQIRGILEGACAGFAAS
                     PDIDISKIEQSYLDAREMTASGDYSRYADLNREFHSEIWAASGNKKMENMIAELWNGL
                     SMGSMVTEEEYAGISIKEHEAIYEAIKAHDVQLAQRRMYEHIMRSRDDMLTYYV"
     gene            complement(4748..5398)
                     /locus_tag="DHV81_02955"
     CDS             complement(4748..5398)
                     /locus_tag="DHV81_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI93476.1"
                     /translation="MRNKRVCDYIAEAIQNEILSGRMSSGVPLRQEELADSLGYSRIP
                     IREALQILESQGLAKRLATRHVVVADFSDEVITEIYELLSDVEIKLLKGMLQSGICLW
                     DKAVSDNSEQPNCVYEPSAINEKLYAELQFHETIISNSHNEYFTHILENGLWCYIRFA
                     VEETAAGRENARRLELLSKIAEAYSDKDIKIVRELLGEYFGSLCEAVIAEREESSR"
     gene            complement(5461..>5682)
                     /locus_tag="DHV81_02960"
     CDS             complement(5461..>5682)
                     /locus_tag="DHV81_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI93477.1"
                     /translation="TGIGIKAEDLPRVFEKGYTGYNGHADKKATGIGLYLCRQMADKL
                     GHTIHMESEIGKGTKVWIGFDLDYADTRD"
BASE COUNT         1484 a         1387 c          962 g         1792 t
ORIGIN      
        1 tatcttacat ttttgtaaga aatgacaaag aaacttaatc tggagtagca aaaagaggat
       61 tagcacagat gtgctgatcc tccttttatc tacagatttt taattacctc aactcttgtg
      121 acaacgccct gcttcacatt atcaactctg agcgcacaat cctggtaata cacacaggct
      181 ccgattgaca catttttacc ctcatcctgc aatttttcgc ttatcacatc tctaagcagc
      241 tttgtctttt cctccggtat atttgttccg gaaataaaat tcgccctcgc taccgtctgg
      301 ctgccacggc atatcatgtg gtctgcctca tcaaagctgc agaaaaggaa ttttctgatg
      361 gcaaaaagcg ccgctatcgc acctattccg acaagtgtct catatacatt gagatgctcc
      421 acgatcatct gtctggctgt cgcaaacaaa agcacctcta tgatcgtgcc cggcttatga
      481 gtacagagca ttttcacaaa ctccacgccg attgcaagcg acatgatata ttccatgtaa
      541 tactccagtt taattttatc atgccatata tcaaaaaaac actgccctat aagctttata
      601 ataagcacca ctatcacaaa caccagtact gccgacagaa aaagttccac gtaggctgct
      661 acatttttca ttgtttttct caggaattgc ttcattatca tttattcctc tcttttttag
      721 ttaccggcaa taatcttttt tattgcagta annnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta atttcatata tctaaaagct cccacataat
      841 agtcaaatta tgtgagagcc tttgcgcgaa aattatattt ttttatgttt tctcccgcat
      901 tctgcgggta tatattttgc cagaatactt tcatggcaat tgggttctaa ataaatttga
      961 taccaggctc cggcaactat ttgatgaatc ctacctgctt gcagatttcc tctgttgctg
     1021 catctttttt cttgttgaac tcaactttct gctgctcgaa ccagttgtta ccctctgtat
     1081 ctatagatac gataagtggg ccaaactctt tcacacggca gttccagagt gtctcaggca
     1141 ttccgagatc tctccactct gcgcggacga tttcctcaac ctctgttgcg gctactactg
     1201 catttcctgc cgggaatacg cagtgaatgg caccgaactc tttacatgca cgctctgtat
     1261 tctctttcat accacctttt ccgatgataa cacgtacgcc ggtctctttt acgaactcgt
     1321 actcgaattt ctccatacgc atacttgttg tcggtccaac agataccatc tcaaatttgt
     1381 cattctcgag tggacggatg atcggtcctg catggaaaat agcattgttt cttacgtcta
     1441 ccggaagttc tcttccctcc tcaacgagac gtcggtgtgc tacgtcacgg catgttgtca
     1501 tgcttccatt taagtataca atatctccta tttttattcc tttaagatcc tctgctgaga
     1561 ttggtgttgt taaaattgtt tttccattaa ttctctctac cattataatt ccacccccga
     1621 atgtgttgtg attgtatagt taaggtcctt atcaaaaaca atgtgtccgc gtctgtggct
     1681 ccagcagcct acgtttacgg ctacaccgat tgtagatgga tgacgggctg tgttctcgat
     1741 atttacaccc attactgagt attttccgcc cattccctga ggtccaaggc cgatagcatt
     1801 gataccatcc tcgagaagtt tttccatctt tgcggctctc tcgttctcat tgtgagatcc
     1861 gattggacgc atgagtgctt tctttgataa aagtgctgct gtctcaacag atgttgctac
     1921 acctacacct accaggagag gtgggcatgc atttaaacca taagtagtca taacatccat
     1981 tacgaatttt gtaactcctt catagccctc tccaggcata agtaccatag cttttcccgg
     2041 aagtgtacat ccacctcctg ccatgtatgt gtagatctcg catttgtcgc tgtttggcac
     2101 gatatcccag aatactgtag gtgttccttt tccaacgttc ttgcctgtgt tgtactcatc
     2161 gaaagtctca acactgttgt gacgaagagg agcctcaaat gtagctttta caactgcctc
     2221 ttttaaaagt ccctcaagct catcaataag tgggaatttt gttccacatt taacccagaa
     2281 ctgaagtact cctgtatcct ggcagcttgg gcggttaagt tttaccgcaa gctcctgatt
     2341 tctaaacatt gtatcataga ttgtttttga aagaggactg tcctctttgt cacggagttc
     2401 tgtcagtttt gcaatgacat catccggtaa tttttttgct atatgagcga taaaatttgc
     2461 catataggtg gtaagctgtt ctacctgttt ctcgtttgac atagtttttc ctcctaatat
     2521 aaatcttgta aataattaaa tattaattta ttgtttaccc actcccatcc gttcttatgg
     2581 gaagaacgga tagaaaatcg gcagtaagac catacttaca atggtcgcca ctactataag
     2641 cggaataccg gcctttgcat aatccttgaa ggtgtatcct ccggccgtaa tgaccattgt
     2701 gttggcaggc ataccgatag gtgtggcgta tgcacatgaa ccaccgataa cacatgccat
     2761 aagtactgca ctcgggtcag ctccaatctc tgtggcaatt gatacgcaga ttggaaccaa
     2821 aagtgctgtg gtggcggtat ttgacataaa gttcgtaagt acacagcaaa gcatgaacac
     2881 tacgaatgtg agaacgtatg gtgatggatt tttgccaagt ataccgataa ccgctgttgc
     2941 gatttcttcg cctgccccgg tctcctgaag cgccgctgca agtgaaagag ttccaccaaa
     3001 taagaaaatg gttttcaaat ctattgattt aagtgcgttt ttctcactaa taacgcctgt
     3061 aatgataagg gcaagtgctc cgacacagcc gatgacacaa agcttgatgc caatcttgtc
     3121 ctcgaaaatc attcccaaaa gtgtaaggat taagataatc aatgacatca tctgtttcca
     3181 tttagggaca tttgaaaaat ccttctgctc atcatatgag ctttccgtac tgccctgctt
     3241 ttcaggcagg aatttgtatc ctataaatgc aaagtacaga atacctgcta taagtatcgg
     3301 aagtcctacc tttgcatact cgaaaaatcc aaagctaagt ccaatctctc ccatagctga
     3361 ttgtgctatg agatttcccg gtgcaccgat gagagataaa tttccaccca tggctgctgc
     3421 aaaaacaagt ggcataagaa gtctcgatct cgagtatcct gattttgctg caattccgat
     3481 aactaccgga atgagaactg ctgcagttcc tgtgtttgaa agtactccgc tcatcagtcc
     3541 cacaatcacc atgattgcta tgataagctg cctctctgtt ttggcgaagt gtgtgaccac
     3601 tcctccaatt ttgtttgcca taccggtttc aaacaatgct cctccgacga taaacatcgc
     3661 tacgaaaaga attacattgc tgtcaataaa tcctgaaaag gctgttttcc aatccagtac
     3721 tcctatgata acaagaccga cacatactat catggatgta accccaagcg gaatcttttc
     3781 ccacacgaac atcacaacgg caaacaataa gaataaaagt gttattgttg ctggactcat
     3841 atcattttcc tcctccgttt aatatttgat atttgatatt gtatccaatt tctaagttta
     3901 agatataaca aaaaaccgaa atcgtcaata cctttttgac aatttcggca atttatatat
     3961 attccaccta ttcaatttgt gcatctatta caagtaatgt gttaaattag caacttacat
     4021 ctacacataa tatgtcagca tatcatcacg actacgcatg atatgctcat acatcctccg
     4081 ctgtgcaagc tgcacatcat gagccttgat tgcttcgtat atagcctcat gctcctttat
     4141 agaaatgccc gcatattcct cctctgtgac catgctgccc attgaaagtc cgttccatag
     4201 ttctgcgatc atattttcca tctttttgtt gccagatgct gcccagattt cactatgaaa
     4261 ctcacggttt aaatccgcat atctgctata atctccggaa gcggtcatct cccttgcatc
     4321 caggtatgac tgctctatct tgctgatatc gatatcaggt gaagcagcaa aacctgcaca
     4381 tgcgccctcg agtatgcctc taatctgata gtgctccctt atgtacgtct ctgtaattcc
     4441 aagtactatt gctcccttat tcggtcttag cttgataagt ccgtcatgcg ctaatatctg
     4501 aaaagcctca cgcacgggag ttatagatac attaagctgc tgcgctacac tttccagtgt
     4561 aagattttct ccctctttta gctgttttga caaaattgcc ttgcgcagct ccgatgctac
     4621 acgctccttc gctgacggaa tctttatcgg tttaagtcct atcataatta ctttttcctc
     4681 ttgttatctt ttaattttat atgcttccga atcatctgtc attattcagt ataaaataag
     4741 tcaccaatta cctgctgctt tcttccctct ctgctataac agcctcacaa agacttccaa
     4801 aatattctcc caaaagttct ctgacaatct ttatatcctt atccgaatat gcctctgcta
     4861 ttttcgataa cagctcaagt cttcgcgcat tttccctgcc tgctgccgtc tcttcaaccg
     4921 caaacctgat atagcaccaa agaccatttt caagaatatg tgtaaaatac tcgttatgac
     4981 tattggatat aattgtctca tgaaactgca gctctgcata caatttttca tttatagcag
     5041 atggctcata tacgcaattg ggctgctcac tgttatcact gaccgcttta tcccacaaac
     5101 atataccaga ctgcagcatg ccctttaaca gttttatctc cacatctgaa agcagctcat
     5161 agatttccgt tatcacctcg tccgaaaaat cagccaccac cacatgcctt gttgccagtc
     5221 gctttgccaa tccctgcgac tcaagtatct gcaatgcctc cctgattggt attctcgaat
     5281 aaccgagact atccgcaagc tcttcctgtc gcaatggcac tcctgatgac atacgtcctg
     5341 ataaaatttc attctgaata gcttccgcta tataatcaca tactctttta tttctcataa
     5401 tatattatta tatctgattt tggatacaat ttccagtcaa acttattaca ccgatttatc
     5461 ctaatccctc gtatctgcat aatccagatc aaaccctatc cacaccttag ttccttttcc
     5521 aatctctgac tccatgtgga ttgtatgccc cagcttgtct gccatctgcc tgcacagata
     5581 aagcccaatt cccgtagcct tcttatcggc atgaccattg tagcctgtgt agcccttttc
     5641 aaaaacacgt ggcagatctt cggctttgat tcctattccg gt
//
LOCUS       DPUF01000082            5891 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_32098, whole
            genome shotgun sequence.
ACCESSION   DPUF01000082 DPUF01000000
VERSION     DPUF01000082.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_32098"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="DHV81_02965"
     CDS             complement(<1..613)
                     /locus_tag="DHV81_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCI93478.1"
                     /translation="MNILDQLAEYSRLRVAEDKKKISLEEMKNIAIQTKNEKLCDFAF
                     EKALKKDDLSFICECKKASPSKGLIEPDFRYLEIAREYEKAGADCISVLTESKWFLGS
                     DEYLKEIAKTVSIPCIRKDFTVDEYQIYQAKTLGAAAVLLICSILSEEQLGEYIKICD
                     SLGISALVEIHNEKEAGMAVRAGARIIGVNNRNLKDFTVDTANS"
     gene            complement(640..1656)
                     /gene="trpD"
                     /locus_tag="DHV81_02970"
     CDS             complement(640..1656)
                     /gene="trpD"
                     /locus_tag="DHV81_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCI93479.1"
                     /translation="MIKEAIVKIVNKEDLTYDEAYAVMNEIMGGETTSTQNAAFLAAL
                     STKSAKAETTDEIAGCAAAMREHAVQVKTGMDVFEIVGTGGDNAQSFNISTTSALVAA
                     AGGMKVAKHGNRAASSLCGTADCLEALGVNIDQSPEKCVELLNKVGMCFFFAQKYHTS
                     MKYVGPIRKELGFRTVFNILGPLTNPGSPAMQLLGVYDEYLVEPLAQVLVSLGVKRGM
                     VVYGMDKLDEISMSAPTKICEIKDGWFRTTVISPEDFGFERCTKEDLKGGTPEKNAKI
                     VRDILGGQKGHKRNAVLMNAGASLYIGGKADSMKEGIELAAEIIDSGKALETLDKLIE
                     VSNS"
     gene            complement(1777..2343)
                     /locus_tag="DHV81_02975"
     CDS             complement(1777..2343)
                     /locus_tag="DHV81_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCI93480.1"
                     /translation="MILLIDNYDSFSYNLYQLIGEIEPDIRVIRNDEMTVEEIRTLKP
                     DRIILSPGPGRPEDAGVIIAGAKELGKEIPILGVCLGHQAICAAYGATVTYAKELMHG
                     KQSDVSFDTESLLFKGCPKKAKVARYHSLAADADTMPDCLKITATTDDGEIMAVEHRE
                     YPIYGVQFHPESIMTPDGKQMLSNFIKA"
     gene            complement(2340..3806)
                     /gene="trpE"
                     /locus_tag="DHV81_02980"
     CDS             complement(2340..3806)
                     /gene="trpE"
                     /locus_tag="DHV81_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCI93481.1"
                     /translation="MKISPTLTEVKEIAKTGKYDILPVSCEILSDFITPIEAMRILQN
                     VSTHCYLLESAQANEAWGRYTFLGYDPKLEINCIDGHMKLGKLSVETENPSEYIRQIL
                     SEYKSPKFDYLPSFTGGLVGYFSYDYLGYKEKSIRGKARNTEDFKDVDLMLFDKVIAF
                     DNLCQKIILIANMSLDAPETGYNKAIVELKQLRELLMHGEKKQNMGGKLLGEVTPLFE
                     KEQYCEMVEKAKHHIKEGDIFQIVLSNRLSVPFEGSLFDTYRVLRTINPSPYMFYFSG
                     TDVEVAGASPETLVKLENGVLHTFPLAGTRPRGATAEEDKKLEEGLLKDEKELAEHNM
                     LVDLGRNDLGKVSKFGSVQVEKLHSIERYSHVMHIGSTVRGEIRDEYDALDAIEAVLP
                     AGTLSGAPKIRACQLIGELEDNKRGIYGGAIGYIDFTGNMDTCIAIRIAYKKNGKVFV
                     RSGAGIVADSDPEKEFQECINKAKASLKALEVSQEVKE"
     gene            complement(3803..3984)
                     /locus_tag="DHV81_02985"
                     /pseudo
     CDS             complement(3803..3984)
                     /locus_tag="DHV81_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516020.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4432..4905)
                     /locus_tag="DHV81_02990"
     CDS             complement(4432..4905)
                     /locus_tag="DHV81_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCI93482.1"
                     /translation="MNIRRAQEKDIPRLIELLEQVLQIHADIRPDIFIPGTTKYTNEE
                     LAEMIKDDTKPIYVAADDDDVCMGYAFCQIRQQPFSNNMVPFTSLFIDDLCVDAKTRG
                     AHIGEQLFEYVKNEAKQLGCYEVTLNVWSGNTSAEKFYEKMGLKTKERQMEYILM"
     gene            complement(4954..5877)
                     /locus_tag="DHV81_02995"
                     /pseudo
     CDS             complement(4954..5877)
                     /locus_tag="DHV81_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743739.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    5616..5700
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1393 a         1471 c         1048 g         1894 t
ORIGIN      
        1 tgctgttagc tgtatcaact gtaaaatcct tcagattgcg gttgttgaca cctattattc
       61 tggcaccggc tcttaccgcc atccctgcct ctttttcatt gtgtatctcc acaagggcgc
      121 ttatgccaag gctgtcgcag atttttatat attcaccaag ctgttcctct gacagtatgg
      181 agcagattaa aagcaccgca gcggcaccga gtgtctttgc ctgatatatc tgatattcat
      241 caaccgtaaa atcttttcga atgcacggaa tcgaaaccgt ttttgcaatc tccttaagat
      301 actcatccga gcctaaaaac cactttgact ctgtcagtac cgagatgcaa tccgcacctg
      361 ccttctcgta ttcccttgca atttcaagat atctgaaatc cggctctata agcccctttg
      421 acggcgaagc cttcttgcac tcgcatatga agctcaagtc atcctttttg agtgcttttt
      481 caaatgcaaa atcacaaagc ttttcgtttt tcgtctgaat cgcgatattt ttcatttcct
      541 caaggcttat ttttttcttg tcctctgcca cgcggagcct tgaatattct gctagctggt
      601 ctaaaatatt catcgctttt cctttcctgt tttgtgtgcc tagctgttgc ttacctcgat
      661 gagcttatcg agtgtttcaa gcgcttttcc tgaatcaatg atttctgctg ctagctcgat
      721 accctctttc atgctgtcgg cctttcctcc tatataaagt gatgcgcctg cgttcatgag
      781 tactgcattt ctcttatggc ccttctgtcc gcctaagata tcacgtacaa ttttcgcatt
      841 tttctcaggt gtaccgcctt ttaaatcctc ctttgtacag cgctcaaagc cgaaatcctc
      901 cggtgagatt actgtggttc tgaaccatcc gtcctttatc tcgcatatct tggtcggtgc
      961 gctcatggaa atctcatcga gcttatccat gccatatact accattccac gctttactcc
     1021 gaggcttaca agcacctgtg caagcggctc tacaagatac tcatcataca ctccgagaag
     1081 ctgcatagcc ggtgaacccg gatttgtgag cggtcctaaa atattgaata ctgttctaaa
     1141 accaagctct tttctgatag gtccgacata tttcattgat gtgtgatatt tctgtgcaaa
     1201 gaagaagcac atgccgactt tatttaaaag ctctacacat ttctcagggc tctggtctat
     1261 gttgacgccg agtgcctcaa ggcagtctgc tgtgccgcat aatgaagagg ctgccctgtt
     1321 tccgtgcttt gccaccttca tgccgcctgc tgccgcaaca agtgcagagg ttgttgaaat
     1381 gttgaagctt tgtgcattgt ctccgcctgt tcctacgatt tcgaacacat ccatacctgt
     1441 tttcacctgt accgcatgct ctctcatggc tgctgcacag cctgcgattt cgtctgttgt
     1501 ctcagccttt gcgctctttg ttgaaagagc tgcaagaaaa gctgcattct gggttgatgt
     1561 tgtctcaccg cccatgatct cattcatcac tgcatatgcc tcgtcatatg tgaggtcttc
     1621 tttgtttacg atttttacta tggcttcttt tatcatgatt atgtcctcct gtattccgat
     1681 gatgtttcgt ttataatttt gtttatgatt ttgttctgct atttgttttc aattcaacaa
     1741 ctaatatata aattcattaa acgctgcaat tttcaattaa gctttgataa aattactaag
     1801 catctgcttt ccgtccggcg tcatgattga ctccgggtga aactgcacgc cgtatatcgg
     1861 atattccctg tgctcaacag ccatgatctc accatcatct gtagtggctg taatctttag
     1921 gcaatcaggc atcgtgtccg catctgcagc aagtgaatgg taacgtgcta cctttgcttt
     1981 tttcgggcaa cccttgaata aaaggctttc tgtgtcaaac gatacatccg attgctttcc
     2041 gtgcatgagc tcctttgcat acgtaactgt cgcaccgtat gctgcgcata tcgcctgatg
     2101 tccaaggcac acgccaagga ttgggatttc ctttccaagc tcctttgccc cagctattat
     2161 cacccccgca tcctcaggcc ttcccggtcc cggtgataat atgatgcggt caggctttaa
     2221 agtcctaatc tcttcaactg tcatctcatc atttctgatt actctgatat caggctctat
     2281 ctcgccgatt agctgataca gattgtaaga aaagctgtcg tagttatcaa tcagtaaaat
     2341 cattctttca cctcctgtga gacttccagt gccttaagag aagcctttgc cttgtttatg
     2401 cactcctgaa attccttctc aggatctgaa tccgcaacta ttcccgctcc gcttctcacg
     2461 aaaaccttgc cgtttttctt gtaggcaata cggattgcaa tacatgtatc catgttccct
     2521 gtaaaatcta tatatccgat cgcccctccg taaatgcctc gcttgttgtc ctcaagctct
     2581 ccgatgagct ggcacgctct gatttttggc gctcctgaaa gtgttcctgc cggaagcaca
     2641 gcctcaatgg catcaagcgc atcatattca tccctgatct cgcctcttac tgtcgaaccg
     2701 atatgcatta catgtgaata tctctctatg gaatgaagct tttctacttg tacacttccg
     2761 aatttgctta cctttccgag gtcatttctt ccaaggtcca cgagcatatt gtgctctgcg
     2821 agctcttttt cgtccttaag cagcccttcc tcaagctttt tgtcctcctc tgctgtggca
     2881 cctctcggcc tcgtgcctgc cagcgggaat gtgtgaagca ctccattttc aagcttcaca
     2941 agtgtctccg gagatgctcc cgcaacctcc acatcggttc ctgaaaaata aaacatgtac
     3001 ggcgaaggat ttatcgttcg aagcacgcgg taggtatcaa aaaggcttcc ctcaaacggc
     3061 acactcaatc tgttggataa aactatctgg aaaatatccc cctctttgat atggtgcttt
     3121 gccttctcga ccatctcaca atactgctcc ttttcaaaaa gcggtgtgac ctcacccagc
     3181 agcttgccac ccatattctg ttttttctct ccgtgcatca gaagctccct aagctgcttt
     3241 agctcaacaa tcgctttgtt gtagcctgtt tccggagcat caagtgacat atttgcgatt
     3301 agtattatct tttggcacaa attgtcaaat gctattacct tatcaaaaag catcagatca
     3361 acatccttga aatcctctgt gttccttgct tttcctctga tgcttttctc cttgtagccc
     3421 agataatcat acgaaaaata acctacaagg ccacctgtaa acgaaggcag ataatcaaat
     3481 ttcggactct tgtactctga aagaatctgt ctgatgtact ctgaaggatt ttccgtctcc
     3541 accgaaagct ttccaagctt catgtgacca tctatgcagt taatctcaag ctttggatca
     3601 tatcctaaaa atgtatatct tccccaggct tcattggctt gtgctgattc aagcagataa
     3661 caatgtgttg aaacattctg aagaatcctc atagcctcta taggtgtgat aaaatctgat
     3721 aatatctcac agcttacagg gagaatgtca tacttcccgg tttttgctat ttctttaact
     3781 tcggtcagtg taggtgaaat tttcatatgc ttgcctccaa attttgtgtg gtagtgaata
     3841 aatgtccaat gttcaaccgt gaacaaatgc ccaacataca agctcgaatt cgccttttca
     3901 aggcttctcc gcagcttacg ctgctacaat tctcgcttta tgttgtgcag tccaatgttc
     3961 gttcgcaagc ttgcatgcga gcatgcagac ttgcaaactc cattgtccct ctttgttcac
     4021 taaaaaacgc ccttgacaga attttagttc tgtcaaggac gaataatctt aactatccgc
     4081 ggtgccacct tgaattcacg ataaagctcg tgcgcttggc aggataccaa catatcccgg
     4141 acaaatgacg tatgccctca cgtcgcagaa tactctggat atctggatga atgataaatc
     4201 catttgactg cgccctcagc ggtccatttg acaacttgtt tcacacctga ttctcagcac
     4261 cacaggctct ctgtaaaggc atcattgccg ttatctccgc ttcaacggtt tacaacttat
     4321 taaattgtcg aaacagttta aactgttttc acttctttaa cgatttaata ctttatctcg
     4381 ctaaatttgt ttaaagttta tcactccttt ttgtgtttgt caacatacat tttacattaa
     4441 aatatactcc atctggcgtt cctttgtctt aagccccatc ttctcataaa atttctcagc
     4501 agatgtgttt ccactccata cattgagggt gacctcatag cagccgagct gctttgcctc
     4561 attcttgaca tattcaaaaa gctgctctcc gatatgcgct cctctggtct ttgcatctac
     4621 acacagatca tctatgaaaa gtgatgtaaa cggcaccatg ttgttggaaa acggctgctg
     4681 tcttatctga cagaatgcat atcccatgca tacatcgtca tcatctgccg caacatatat
     4741 cggctttgta tcgtccttta tcatttctgc aagctcttca tttgtatatt ttgtcgttcc
     4801 cggaataaaa atatccggtc tgatatcggc atggatctga agcacctgct ctaacaattc
     4861 tataagtctt ggtatgtctt tttcctgtgc tcttctaata ttcatttttg agaatcatcc
     4921 ttcctgttgt cgttaatgta tagtttttat atttcaaaat aaatccagca tattatcgag
     4981 ataaatgtgc tctctgacat gtatctgata ctgtggcttc gtcatatagt agagcagaaa
     5041 ttcaagtatc atggcacttc caagccctct gccctctatt ttaaaattgc taaagcccat
     5101 cggcagataa gtgttcttta tatcgtctac tcctataaat cctggatttt ccatcgcctt
     5161 cgaaaagaga tatccctgcc ccgcatcagg cgcagtgcaa tagtgctccg agcaatcctc
     5221 gcccagattt ttgcggctaa ccgtctcgta gcatttcttc ctgtccgtgc agccaaacca
     5281 gcagcactca ttgcacaaaa attctacttt ttccttttga tgctgtgata gtgctttcag
     5341 attgtcaaat gatttgttca acctgaaatc cggtactaca tagcggaatt cctctctgtt
     5401 tagctcattt ttaaattcac tgaaatccgt cagaaccttt gtggttgaag acacaaaata
     5461 aaagtcaggg tagttttctt ttatgtaatc aagcagaata tctgagtaga tgattattcc
     5521 gtttccaaag cccctgcttc gctcattatc tctgcctata tcaaatatcc tgcacagttc
     5581 gttacatttt ttgtcaggaa gatgctcctt agtaannnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 gtgctcacca aaaccgacgc ggcctccgcc ccatatgcag tctgccggtg caccgtatat
     5761 tgagcctatg tcgcaccagt catagaaata ttcccggtgc ttgcggtaca gtgggagaaa
     5821 ttctttgtat aattcataaa attcaaacaa tcccggtaga tgataatacg ctttcatttt
     5881 gactccttat t
//
LOCUS       DPUF01000083            8747 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_28786, whole
            genome shotgun sequence.
ACCESSION   DPUF01000083 DPUF01000000
VERSION     DPUF01000083.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_28786"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="DHV81_03000"
     CDS             <1..663
                     /locus_tag="DHV81_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCI93483.1"
                     /translation="EFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGSQLKRLG
                     EYYAKAIHANYGDDFDVLFGPAYKGIPLAVVTAIAYHELYGKEVRYCSDRKEAKDHGA
                     DKGGFLGSKLKDGDRVVMIEDVTTSGKSMEETVPKVREAADVTIVGLIVSLNRMEVGQ
                     GGKVSALDEIHEKYGFEGKAIVTMAEVTEYLYNREHDGRVVIDDTIKAAIDEYYKQYG
                     CK"
     gene            675..1004
                     /locus_tag="DHV81_03005"
     CDS             675..1004
                     /locus_tag="DHV81_03005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93484.1"
                     /translation="MLGRRKRNRYKFTEKKQSKRGIAALVVAILIEIVYIVFLNAAFR
                     GDGNLSMYYGSAGILMLGISLADFGFAIRSLFDEESFMTFPRLAVFFALIAVLSWGGT
                     YAAGFII"
     gene            1079..>1461
                     /locus_tag="DHV81_03010"
     CDS             1079..>1461
                     /locus_tag="DHV81_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCI93485.1"
                     /translation="MSYKEIYELSNELYAERLELVEERIEQIIREPAIEPAFADYFRS
                     AAKCINTIKNHSADKEFNDLFYSQFDKENYEKSYANPAYAVKVLGDEYGQLLSAVYAK
                     IAGSITHIYQGDIKYLCIYAELIVEL"
     assembly_gap    1462..1590
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1591..3089
                     /locus_tag="DHV81_03015"
     CDS             <1591..3089
                     /locus_tag="DHV81_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCI93486.1"
                     /translation="AYDYYTELVNEADLSNDDYLYSYGLYVGENERAGRAHLASFSDE
                     EIQAMADTYTEGYRIGFITCNKDISKKSVVQVIYPLGFERMIRAALKNFEKMGMKPAM
                     RPFSTSVNKQFDYDHKEDMALWLDKAYVEYRLECMHNALERMKDVACKCGGPAVIEIF
                     GEEPFAPVSKKEAAHFNDEQQKLVVHMTSVRSQYMNSYIHSEDRSFTIIAYPCAAIGP
                     DYKEIFTETVKINTLDYALYRDMQQKIIDVLDTADRVHIVGTNGNRTDLYVKIHELKE
                     PSKETAFENCVADVNIPVGEVFTSPVLEGTNGKLHVSQVYLNELNFLNLEIDFKDGMI
                     DKYTCTNFEDEEENKKYISDNVLFHHDTLPMGEFAIGTNTTAYRMARVYDIAAKMPIL
                     IAEKTGPHFAVGDTCYTYDEDNMTYNPDGKAIIARDNSVSIRRKEDISKAYFNCHTDI
                     TIPYDELGAITVIRHDGSTCDIIRDGRFVLEGVEELNKPLDTLDAESK"
     gene            3115..3627
                     /gene="purE"
                     /locus_tag="DHV81_03020"
     CDS             3115..3627
                     /gene="purE"
                     /locus_tag="DHV81_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCI93487.1"
                     /translation="MAKVGIVMGSDSDLKVMSKAADMLEELGIEYELTIISAHREPDE
                     LIEWTRGAENRDVKVIIAGAGMAAALPGMCAALFALPVIGVPLSGKNLDGMDAVFSIM
                     QMPPGVPVATVAIDGAKNAAILAAKILAAGDDAILEKIKKYTADMKAQVVAKDAKLKQ
                     VGYKQYLAEK"
     gene            3657..5111
                     /locus_tag="DHV81_03025"
     CDS             3657..5111
                     /locus_tag="DHV81_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI93488.1"
                     /translation="MGILQKADRCMDEAAALFGENKLFLAENKAQETAGLYKSCGAYE
                     QMAKTVNFMGVIYASIGDLSMSIDCYLEAMDVAVEQGSTEIIMLVNNNIGSLYMELGL
                     YEKAIRYFNEALELCKPPLHGERDSYYQELLMLHLNLCISYTGINEFEKAEKHLSDAI
                     LFNEIAGSDKNRFLIDMSQAHMLWKMGNEDEVRDHVEELVEGAINNINSDNYVLEILS
                     LCNLFMNMGEFDAWKKVIVEYERFATDTQNLFFQKTCVKMWMKYESAMGDTEAYNKLC
                     VYYANLHSMQVKEQIKRLGDTIDLKLQLQETEYERRKAVRLNYTDMLTGMGNKFKMRN
                     DFEKLVSKNQDSDGAGITFGVVDIDFFKSFNRNSGIVTGDAVLKELSSIINESIKDKG
                     MGYHFYGDEFYIILQETDEDIIKNIAEHIRLELAKKQILHESSKVDEYLTVSQAYVTA
                     PDVKVPDEFSKLFKTVDEVLNDVKEKGRNAYKIV"
     gene            complement(5278..6066)
                     /locus_tag="DHV81_03030"
     CDS             complement(5278..6066)
                     /locus_tag="DHV81_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCI93489.1"
                     /translation="MLQLERQQKILDYLKENRKATTNELSELLGVSTTTIRTDLNQMD
                     KEKLLTKTHGGAIYKDKTHDMTDLTSRAYLFHDRALENKEAKEEIANEAIKLVSDHMC
                     IFLDASSTAYTLGMKLSGFTELTVITNGINLALELKDIPGVTVILTGGIVTSASASIE
                     GLLGADLLKKIHTDIAFVSARGFSVEDGLTDFSIYEADLKHMCIKASAKTVALVDHTK
                     FDTTSISSYASLDDINMVITDSGISPETADIYEKAGTDIVISGI"
     gene            6330..6983
                     /gene="fsa"
                     /locus_tag="DHV81_03035"
     CDS             6330..6983
                     /gene="fsa"
                     /locus_tag="DHV81_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCI93490.1"
                     /translation="MKFFVDTANVEDIKKANDMGVICGVTTNPSLIAKEGRDFNEVIK
                     EITSIVDGPISGEVKATTVDAEGMIKEGREIAAIHPNMVVKIPMTVEGLKAVKVLSSE
                     GIKTNVTLIFSANQAILAANAGATYVSPFLGRLDDISQPGIELIRQISEIFDIYGYDT
                     EIIAASIRNPIHVTDCALAGADIATIPYKVIEQMTKHPLTDQGIEKFQADYRAVFGD"
     gene            7049..7888
                     /locus_tag="DHV81_03040"
     CDS             7049..7888
                     /locus_tag="DHV81_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCI93491.1"
                     /translation="MNKLELQITANEVRKGIVTAVHSAKAGHPGGSLSAADIFTYLYF
                     EEMNIDPKNPKKQDRDRFVLSKGHTAPGLYSALAQRGYFPVEDLKTLRHLGSYLQGHP
                     DMKHIPGVDMSSGSLGQGISAAVGMALSAKLTNDDYRVYTLLGDGEIQEGQVWEAAMF
                     AGFRRLDNLVVIVDNNGLQIDGPVDQVCSPYPIGEKFKAFNFHVIDLKDGNDMDQIAD
                     AFKEARNTKGMPTAIIAHTVKGKGVSFMENQVGWHGKAPNDEEYAVAMEDLRKEGEAL
                     CQK"
     gene            7876..>8747
                     /locus_tag="DHV81_03045"
     CDS             7876..>8747
                     /locus_tag="DHV81_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCI93492.1"
                     /translation="MSEVKKIATRVSYGNALVELAKEHDDVYVLDADLAAATQTAIFK
                     KEFPDRHIDCGIAECNMMGIAAGLAATGKVPFASSFAMFAAGRAFEQVRNSIGYPHLN
                     VKIGATHAGISVGEDGATHQCNEDIALMRAIPGMVVINPSDDIEARAAVKAAYEHEGP
                     VYMRFGRLATPIINDNAEYKFEIGKGVTLREGTDVAIIATGLCVAESLAAAEKLAADG
                     VNAKVINIHTIKPLDEELVVAAAKECGRVVTVEEHSVIGGLGAAVCETLSRKAPTPVK
                     TIGIQDCFGESGPAV"
BASE COUNT         2618 a         1545 c         2158 g         2297 t
ORIGIN      
        1 gaatttatag actttatggt ggagagtgac gtgctgaaat ttggtgattt cacattaaag
       61 agcggccgta aatcaccatt tttcatgaat gcgggagcat atgtgacagg ctcacagctt
      121 aagagactcg gagagtacta cgcaaaggct atccatgcta actacggaga tgactttgat
      181 gtattattcg gacctgcata caagggaatt ccgcttgcag tggtaacagc tatcgcatac
      241 catgaactct atggcaaaga ggtgcgctac tgctcagaca gaaaagaggc aaaggatcac
      301 ggtgcagaca agggaggctt ccttggaagc aagcttaagg atggcgacag agtagtcatg
      361 atagaggatg tcaccacatc cggcaaatca atggaggaga cagttccaaa ggtaagagag
      421 gctgctgatg tcacaatcgt aggccttata gtgagcctta acagaatgga agtcggacag
      481 ggcggcaagg tgagtgcgct tgacgaaatc catgagaagt acggctttga gggcaaggca
      541 atcgtcacaa tggcagaggt cacagagtat ctctacaaca gagagcatga tggcagagta
      601 gttattgatg acacaatcaa agcagctata gatgagtact ataagcagta cggctgcaaa
      661 taggagaata taaattgctt ggcagaagaa aacgtaacag gtataagttt acggaaaaga
      721 agcaatctaa gagggggata gctgcacttg ttgtggctat cctcatagag attgtatata
      781 tagttttttt gaatgcggca ttcagaggag atggcaatct gtctatgtac tatggcagtg
      841 ccggtatact gatgcttggc atatctctgg cagatttcgg gtttgccata agaagcctct
      901 ttgatgagga atcattcatg acatttccac gattggcagt gttttttgca cttatagcgg
      961 tgctcagctg gggtgggacg tatgctgccg gctttattat ttagcacata ttatatcaat
     1021 agtgctataa aactgtcatg aatatttgaa ttataaaata gaaagattat agggatatat
     1081 gagttataaa gagatttatg aactctccaa tgagctttac gcagagcgat tagagcttgt
     1141 ggaggagcgg atagaacaga ttatcagaga gcctgcgata gagccggctt ttgcagatta
     1201 tttcagaagt gcggcaaagt gcatcaacac tattaaaaac catagcgcag ataaagaatt
     1261 taacgattta ttctattcac agtttgataa agaaaattac gagaaaagct atgccaaccc
     1321 ggcatatgcc gtaaaggtgc tcggagatga gtatggtcag cttttatcgg ctgtatatgc
     1381 aaaaattgcc ggaagcatta cacatatcta tcagggggat ataaagtatc tctgcattta
     1441 tgcagagctt attgtagagc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggcttatga ttactataca gagcttgtaa
     1621 atgaggcaga tttgtcaaat gatgattatc tgtattccta cggactgtat gtcggagaga
     1681 atgagcgcgc cggcagagcg catctggctt cattttcaga tgaggagata caggccatgg
     1741 cagataccta tacggaaggg taccgcattg gctttattac ctgcaacaag gatatctcaa
     1801 agaagtcagt agttcaggtg atttatccgc ttggttttga gcgtatgata agagcagcat
     1861 taaagaattt tgagaaaatg ggtatgaagc ctgccatgag accattttcc acatcggtca
     1921 acaagcagtt tgactatgac cacaaggagg atatggcact gtggcttgac aaggcatatg
     1981 tggagtaccg tcttgagtgc atgcacaatg cacttgagag aatgaaggat gtagcatgta
     2041 aatgtggagg accggctgtc atagagatat ttggagagga gccgtttgct ccggtttcaa
     2101 agaaggaggc tgcgcacttt aatgatgagc agcagaagct tgtagtccat atgacaagcg
     2161 tgcgctcaca gtatatgaac agctatatcc acagcgagga cagaagcttt acgattattg
     2221 cctatccgtg tgctgcaatc ggacctgatt ataaggaaat ttttacggag actgtaaaga
     2281 ttaatactct tgattatgca ctctacagag atatgcagca gaaaattata gatgtgctcg
     2341 atacagcaga cagggtgcat atcgtcggaa caaacggcaa caggacagat ttgtatgtca
     2401 agattcatga gcttaaggag ccttcaaagg agacggcttt tgaaaactgt gtggctgatg
     2461 taaatatccc tgtcggagag gtgtttactt caccggtgtt agagggcaca aacggcaagc
     2521 tgcatgtcag tcaggtatat ctcaatgagc ttaatttcct taatctggaa atcgacttta
     2581 aggatggcat gattgataaa tatacatgca ccaattttga ggatgaggaa gaaaacaaaa
     2641 agtatatatc agacaatgtg cttttccatc atgacacact gcctatgggt gagtttgcca
     2701 taggcaccaa tacgacagcc taccgtatgg cgagagtata cgatatcgca gcaaagatgc
     2761 ctattctcat agcagagaag acaggaccgc actttgcagt gggtgatacc tgctacacat
     2821 acgatgagga caatatgaca tacaaccctg acggaaaagc aatcattgcc agggacaatt
     2881 cagtttccat ccgcagaaaa gaggatatat caaaggcata tttcaactgc cacacggata
     2941 tcacaatacc atatgatgag cttggagcca tcacagttat tcgacacgat ggctcaacat
     3001 gtgatataat aagagatggc agatttgtgc ttgagggcgt agaagagttg aacaaaccgc
     3061 tcgatacgct tgatgcagaa agcaaataat tgttagatgt ataaggagga taaaatggca
     3121 aaggttggaa tagttatggg cagcgactca gatttaaagg tgatgagtaa ggcggcagat
     3181 atgcttgaag agctcggcat agagtatgag ctcaccatca tctcggcaca ccgtgagccg
     3241 gatgagctta ttgagtggac aagaggcgct gagaatagag atgtaaaggt aataatagca
     3301 ggagcgggaa tggcagcagc acttccggga atgtgtgcag cgctttttgc acttcctgtt
     3361 atcggagtac cgctttcagg aaagaatctg gatggaatgg atgcagtgtt ctcaatcatg
     3421 cagatgccac ctggagtgcc tgttgccaca gttgcaattg acggtgcgaa aaatgcagca
     3481 atccttgcag caaagatact cgcagcaggt gatgatgcaa tccttgagaa aatcaaaaag
     3541 tatacagcag atatgaaggc tcaggttgta gcaaaggatg cgaaactgaa acaggttgga
     3601 tataagcagt atcttgctga gaaatagatt gtattgtcat ctataggagg gtttttatgg
     3661 gtatattgca aaaggcagac agatgtatgg atgaggcagc agccttattt ggcgagaata
     3721 agcttttttt ggcagagaat aaggcacagg agacagccgg tctttataag agctgtggcg
     3781 catatgagca gatggcaaag actgtcaatt ttatgggagt catatatgca tctattggag
     3841 acttaagcat gtcaattgac tgctacctag aggctatgga tgtagctgtg gagcagggct
     3901 caacagagat aatcatgctt gtaaacaata atataggctc actctatatg gagcttggat
     3961 tgtatgaaaa ggctatcaga tatttcaacg aagcactgga gctgtgcaag ccaccgctgc
     4021 atggagagag ggatagctat tatcaggagc ttctcatgct tcatctgaat ctgtgcatat
     4081 catacacggg aattaatgag tttgaaaagg cagaaaagca tctttctgac gcgatactct
     4141 ttaatgagat agctggcagt gataaaaaca gatttctgat agatatgtca caggcacata
     4201 tgctatggaa gatgggaaat gaggatgagg tcagagatca tgtggaagag ctggttgagg
     4261 gtgcgataaa caatatcaat tctgacaatt atgtacttga gatactaagc ctttgcaatc
     4321 tatttatgaa tatgggagag ttcgatgcat ggaaaaaggt tattgtggag tatgaacgct
     4381 ttgccactga tacgcaaaat ctgtttttcc agaagacctg tgtcaagatg tggatgaaat
     4441 atgagagcgc catgggtgat acagaggcgt acaacaagct gtgcgtatat tacgcaaatc
     4501 tccactctat gcaggtgaag gagcagataa agcgtcttgg tgatacaatc gaccttaagc
     4561 ttcagcttca ggagactgag tatgagcgcc gtaaggcagt cagactcaac tacacagata
     4621 tgcttacagg catgggcaat aagttcaaga tgcgaaacga ttttgaaaag cttgtgagca
     4681 aaaatcagga ttcagatggt gcaggaatca cgtttggagt agtggatata gatttcttca
     4741 agtcatttaa cagaaacagt ggcatagtca ccggtgatgc agtgctaaaa gaattgtcat
     4801 ccataataaa tgagagcatc aaagataagg gaatggggta tcatttctat ggcgatgaat
     4861 tctacataat cctgcaggaa acggatgagg atatcattaa aaatatagcc gagcatataa
     4921 gacttgagct tgccaaaaaa cagatacttc atgagagctc taaggtggat gagtatctga
     4981 ctgtatcaca ggcatatgtt accgccccgg atgtaaaggt gccggatgag ttttcaaagc
     5041 tgttcaagac ggtggatgaa gtgctcaatg atgtgaagga aaagggtaga aatgcatata
     5101 agatagtctg atgctttaag cgtataagac aactataaaa tgacacgcag cgtaaaacgt
     5161 tgcgtgtcat ttttaatgga tctatatata cagttatagt tatatttgta cttatattta
     5221 tagttatagt tatatttgta cttatacttt tacttttata tggctatatt gtctcattta
     5281 tattccggat ataacaatat cagtgccggc cttctcatat atatctgctg tctcaggtga
     5341 gataccggag tcggtgatta ccatattgat gtcatcaagc gatgcatagc tagatatgga
     5401 tgtggtatca aacttggtgt ggtctacaag agccacagtt ttagcggagg ccttgataca
     5461 catgtgcttt aaatcagcct cgtatatact gaaatcagtc agtccgtctt ctacagagaa
     5521 gcctcttgct gacacgaacg cgatatctgt gtgtatcttt ttgagtaaat cagcgccaag
     5581 cagtccttct atggaagcgg atgcactggt tacgataccg cctgtcagga tgactgtgac
     5641 acccgggata tcctttagct ctaaagccag attgatgcca ttggtgatga cggtcagttc
     5701 tgtaaagcct gaaagcttca tgccgagagt gtaggctgtg gagctggcat caaggaatat
     5761 acacatatgg tcacttacga gctttatcgc ctcgtttgca atctcctctt tagcttcctt
     5821 gttttccagg gcgcggtcat ggaacaggta ggccctcgag gttaaatcag tcatgtcatg
     5881 ggttttatcc ttgtagatag caccgccgtg agtctttgtc agaagctttt ccttatccat
     5941 ctggtttaaa tctgttctta ttgtcgtggt cgatacacca agcagttcac tgagctcatt
     6001 ggtggtggcc ttgcggtttt cttttaaata atcgagaatc ttttgttgtc tttctaattg
     6061 cagcatagat tttcctcttt cttttgattt ctgttgtatt tttacgtaca tatttttatc
     6121 acaaagcatt gcataaatat ataataactc aaataagtaa ataaagccag tattttcaat
     6181 tatttatgtt cgcttttgtt tggcgatatt taattatatt tatttttact tttatttgct
     6241 tttgctattg cattttttct tttaaagtgt tatatttata tcaacaaata aaagcaaaca
     6301 caaatcatat aaggcggagg tattattaaa tgaagttttt cgtagataca gcaaatgtcg
     6361 aggacattaa gaaagcaaac gatatgggag tcatctgtgg agtcacaaca aacccttcac
     6421 tcatcgcaaa agagggcaga gacttcaatg aggtaattaa agaaatcaca tctattgttg
     6481 atggaccgat aagcggagag gtaaaagcca caacagttga tgcagagggc atgataaaag
     6541 aaggacgtga aatcgcagct atccatccaa acatggtagt taagattcct atgacagttg
     6601 agggacttaa ggctgtcaag gtgctttcat ctgagggcat caagacaaac gttactctta
     6661 ttttttcagc aaatcaggca attcttgctg caaatgcagg tgctacatat gtttcacctt
     6721 tccttggcag acttgatgat atcagccagc cgggcattga actgatcaga cagatttcag
     6781 agatttttga tatctatgga tatgacacag agattattgc agcttcaatc agaaacccga
     6841 ttcatgtcac agactgtgca cttgcaggag ctgatatcgc aacaattcct tacaaggtta
     6901 tcgagcagat gacaaagcat ccacttacag atcagggaat cgagaagttc caggctgatt
     6961 acagagctgt atttggggat tagatattat aaaccaaaga caggtataaa tattttattt
     7021 catgtcatag atttaacagg agaacaaaat gaacaaatta gagcttcaga ttacagcaaa
     7081 tgaagtcagg aagggtatcg tcacggctgt gcattctgca aaggcggggc atccgggcgg
     7141 ctcactttct gcagcagata tatttacata tctgtatttt gaggagatga acattgatcc
     7201 aaagaatcct aaaaagcagg atagagaccg ttttgtgctt tcaaagggac acacggctcc
     7261 gggactttac tctgcacttg cgcagagagg ctattttccg gttgaggatt taaagacact
     7321 cagacatctg ggatcatatc tgcagggaca tcctgacatg aagcatatcc cgggtgtgga
     7381 tatgtcaagc ggttcgctcg gacaaggcat ttctgcggca gtgggcatgg cactttctgc
     7441 aaagcttaca aatgatgatt acagagtata tacacttctc ggcgatggcg agattcagga
     7501 gggacaggtc tgggaggctg ctatgtttgc gggcttcaga aggcttgata accttgttgt
     7561 tatagttgat aacaacggac ttcagattga tggaccggtg gatcaggtgt gctcaccata
     7621 tcctattggc gagaaattca aggcattcaa tttccatgtg attgatctga aggatggaaa
     7681 tgacatggat cagattgctg acgcatttaa agaggcaaga aatacaaagg gtatgccgac
     7741 ggcaattata gcacataccg taaagggcaa aggtgtttca tttatggaaa atcaggtcgg
     7801 ctggcatggc aaggctccaa atgatgagga gtatgcagtt gccatggagg atctgagaaa
     7861 ggaaggtgaa gcattatgtc agaagtaaag aaaatcgcca cacgtgtcag ctacggaaat
     7921 gcacttgtag agcttgcaaa ggagcacgat gacgtatatg ttcttgatgc tgaccttgcg
     7981 gctgccacac agacagccat cttcaaaaag gaatttccag atcgtcacat agactgtgga
     8041 atcgcagagt gcaacatgat gggcattgct gcaggacttg cggctacggg aaaggtgcca
     8101 tttgccagtt catttgccat gtttgcggca ggtcgtgcgt ttgagcaggt gagaaactct
     8161 attggatatc ctcatctcaa tgtgaaaatc ggtgcgacac acgcaggtat ttcggtagga
     8221 gaggatggag ccacccacca gtgcaacgag gatattgcac tcatgagagc aattccaggc
     8281 atggttgtaa taaacccaag cgatgatata gaggcaagag ccgccgtgaa ggcagcctat
     8341 gagcatgagg gccctgtata catgaggttt ggccgtcttg caacaccgat aataaatgac
     8401 aatgcagagt ataaatttga aatcggaaag ggtgttacac tccgtgaggg cacggatgta
     8461 gctatcatag ctaccggtct gtgcgtggca gagtcacttg cagcggcaga aaagcttgca
     8521 gcagatggcg taaatgcaaa ggtcataaat atacacacta tcaagcctct tgatgaggag
     8581 cttgttgtag ctgcagcaaa ggaatgcggc agggtggtta ccgtggagga gcactctgta
     8641 ataggaggac ttggagccgc ggtatgtgag acactttcgc gtaaggctcc aacaccggta
     8701 aagacaatag gcatacagga ctgctttggt gagtcaggtc cggctgt
//
LOCUS       DPUF01000084            7700 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1192, whole
            genome shotgun sequence.
ACCESSION   DPUF01000084 DPUF01000000
VERSION     DPUF01000084.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1192"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="DHV81_03050"
     CDS             complement(<1..1307)
                     /locus_tag="DHV81_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008750103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCI93493.1"
                     /translation="MSIDQSKIRNFCIIAHIDHGKSTLADRIIEKTGTLTSREMQAQV
                     LDNMDLERERGITIKSQAVRIIYTAKDGEEYIFNLIDTPGHVDFNYEVSRSLAACDGA
                     VLVVDAAQGVEAQTLANVYLALDHDLDVFPVINKVDLPSARPDEVAQEIEDVIGIEAM
                     DAPRISAKTGLNIEDVLEQIVKKIPAPTGDKDAPLKALIFDATYDSYKGVIIFCRLRE
                     GSVKVGDTIKMMATGASDVVTEVGYFGAGQFIPCDELSAGMVGYIAASIKNVRDTRVG
                     DTVTLVDRPCDEALPGYKKVNPMVYCGLYPADSAKYPDLRDALEKLQVNDASLQFEPE
                     TSLALGFGFRCGFLGLLHLEIIQERLEREFDLDLVTTAPSVIYKIHKTSGEVIDLTNP
                     SNMPDPSEIEYMEEPYVSAEIMVTSDYVGAIMKLCQERRGVYI"
     gene            complement(1371..2468)
                     /locus_tag="DHV81_03055"
     CDS             complement(1371..2468)
                     /locus_tag="DHV81_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HCI93494.1"
                     /translation="MGDDIVKKKMIVLIAFIYIASMLFCGDGVGKKFVEEKFPHLTYS
                     DKECDDSKKKELRIVEDEDTAGGILSYEELAAMENENASVRNNMLPDTDQLAFVDEAE
                     KNGELAELEEQQNVKMPQVDMSFDNLVKNYYQVDKTTYIGADELCEEALLGRDMSIEK
                     SGNGPDILIYHTHSQEGYSDSLDTSETETVVGVGDRLESLLREKYGYNVLHHKGQYDV
                     NDRDHAYSNSLPEITEIIEKNPSIKVVIDLHRDGVAETTRLAATIDGKPMAQIMFFNG
                     LCRTAARGPINSLPNEYLGDNLAFSFQLQLMANEYYPGLARRIYLKGYRYNMHLCPKS
                     LLVEVGAQTNTKEEAYNAMEPLADILDKVLK"
     gene            complement(2540..3451)
                     /locus_tag="DHV81_03060"
     CDS             complement(2540..3451)
                     /locus_tag="DHV81_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HCI93495.1"
                     /translation="MSADKWCARTDLALETQESLKKANTDMRGVNFSEKKLDNGIIVS
                     VVTIDSENAVRATGRPKGKYVTIEAAMLSEGDEECCQAVTRELSRELKSFVKAVCDKR
                     IYAALVVGLGNRNVTPDALGPRCVDSLFITRHIVKEYGRYAFSNENVNSVCGLVPGVM
                     AQTGMECLEIIKGVVSEVKPDFVVTIDALAARSTKRLGCTIQLTDTGIVPGSGVGNHR
                     DGINHDNLGVPVIAIGIPTVIDAVTIVSDAVNASRENTAKLMSPKLNGMFVTPKDIDE
                     TVKRLAGLLSEGINMAFSNDEYDDYSE"
     gene            3617..3895
                     /locus_tag="DHV81_03065"
     CDS             3617..3895
                     /locus_tag="DHV81_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCI93496.1"
                     /translation="MEVYELANIKSAKKRILVTETKAARNKAIKSGVKTAIKKVEAAV
                     AANDKAAAEAALKDATSVIASAGSKGIFHKNNAARKVSRLTKLVNGLA"
     assembly_gap    3941..3990
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3991..4975)
                     /locus_tag="DHV81_03070"
     CDS             complement(<3991..4975)
                     /locus_tag="DHV81_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCI93497.1"
                     /translation="MKTIDNDIKAGDFKKVYLLYGQERYLIKQYRDKLIKAMVSEGDS
                     MNFSSFEGDGINQKEIIDLAETLPFFADKRVILIEDAGIFSKAGDELGEYLKDSPEST
                     HFIFVEESVDKRSKLYKAAAKCGNPVEFKEQTDETLARWIGMRIRKDGKGMSQAAYNS
                     FIEKTGTDMENIDKELEKLLCYCMDKDVIELSDVEAVTTEQITNKVFEMVDAIASHKQ
                     KRALELYYDLLALKEPAMRIMYLISRQFNILMNVKAMTNKGFGNKDIASKAGCPEWAV
                     RKYQAQCRAYSLDDLKMAVRDGVAYEEDVKTGRMNDQMAVELFIVQYSADMT"
     gene            complement(4956..7319)
                     /locus_tag="DHV81_03075"
     CDS             complement(4956..7319)
                     /locus_tag="DHV81_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCI93498.1"
                     /translation="MFLFALVYTAGTVSGMYGRGFGFLTFAILLVILIAECVASKGSF
                     SGVRRIVVLLILISSAVFATAQFRYASVDENRDSYMQYMTDEKSVIVWGKIYKTEYKY
                     FSYRYYMTDCVVQPGYGKAFGRKISCGDVVVYCDSKSAHLGDYMKACGKIGLFKSATN
                     EGEFDRARFYRSQGIDFSVNADSIKTSAGKHAWYYHRLEKLRDTLSASLLEVTDETTA
                     GVLSSMLLGDKSYLDDEIKDLYQVSGISHILAISGLHISIVGMAFYKLLRKRRVSYTA
                     AFVCSAALILSYAVMTGNAVSTRRAVGMFILTMLAAALGRCTDMLNSLGIMVIYLLWD
                     NPMVLEYAGFVFSVGAILAIGIVTPVISAPKGGKFWASVSIQLPMLPVVAMNYYELPL
                     FAVFVNLIVIPALPVVFISGLLASAAGCFGVMPGKLLVFPAFAVLKLYEVLCGLVMKL
                     PGASVITGAPDGYRIFLFYAVMSGFLVAFSLRKRAIECGSKEKGQILYSVQRVVCTAV
                     LLAILLVKPKTAAQLDILDVGQGDGIFYRFESGICIFIDGGSSDRKQLGENVIMPFLK
                     YNGIQSISYWFVSHADSDHISGLAEVIDSGYTIEHIVVAEAAAKEEATEELLFKAKEA
                     GIDICLMSKGDSIEINDASGSRSTANEEADGIMCLYPGPYDTALDRNDMCLVLKLTDG
                     RFSGIFGGDISSEVEKKLVNEYGDELSVDFYKANHHGSRYSSSADWIEALSPRWAAVS
                     CAAENRYGHPADEAMDRLMDAKCRILYTMQSGQIKYKESAIYENNRQ"
     gene            complement(7362..>7700)
                     /locus_tag="DHV81_03080"
     CDS             complement(7362..>7700)
                     /locus_tag="DHV81_03080"
                     /inference="COORDINATES: protein motif:HMM:PF10646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation and spore germination"
                     /protein_id="HCI93499.1"
                     /translation="VVEQLIEGPKGSKLKATLPGTTKLINVSVADRICYLNFDSSFRN
                     TIDNKLTEDVVLYSIVNSLTSLPTVDKVQISLDGENDGMLLYNYKLSDMYEFNKDIVD
                     SDDSTEKTEK"
BASE COUNT         2098 a         2040 c         1354 g         2158 t
ORIGIN      
        1 ctgatataga ctcctcgtct ctcctggcac agcttcataa tcgcaccgac atagtcactt
       61 gtgaccataa tctctgcaga tacgtatggc tcctccatat actcaatctc tgacggatcc
      121 ggcatatttg aaggattggt cagatctatc acctcaccgc ttgtcttgtg aatcttgtaa
      181 ataacactag gtgcagtggt aacgagatcc agatcaaact ctctctccag tctctcctga
      241 ataatttcca gatgtaaaag accaaggaat ccgcatctga agccaaagcc aagcgccagt
      301 gacgtctcag gctcaaactg taatgaagcg tcgttgacct gcagcttttc aagcgcatcc
      361 cttaaatcag gatactttgc actgtctgca ggataaagac cacagtaaac catagggttg
      421 accttcttat agcccggaag cgcctcatcg cacggtctgt caacgagtgt aactgtatca
      481 cccacgcgtg tatccctgac atttttgata ctggccgcaa tgtatccaac cataccggcc
      541 gaaagctcat cgcatggtat aaactggcct gcgccaaaat accccacctc tgtaaccaca
      601 tcagatgcac ctgtagccat catctttatt gtgtctccta cctttacaga gccctctctc
      661 agtcggcaga aaataatgac tcccttataa gaatcatatg ttgcatcaaa tataagcgcc
      721 ttaagcggtg catctttatc gcctgttggt gccggaatct tttttacaat ctgctccagc
      781 acatcctcta tattgagtcc tgtctttgcg gaaatacgcg gtgcatccat agcctcaata
      841 ccgataacat cctcaatctc ctgtgccacc tcatccgggc gcgcactcgg cagatccacc
      901 ttatttataa caggaaatac atccaggtca tgatccagtg caaggtaaac atttgcaagt
      961 gtctgtgcct caacgccctg ggccgcatcc actacaagta cagctccgtc acatgccgca
     1021 agactccttg atacctcata gttaaaatcc acgtgtcccg gtgtatctat cagattaaaa
     1081 atatactctt cgccatcctt tgcggtatat ataatacgga ctgcctgaga cttgattgtg
     1141 ataccacgct ctctctctaa atccatattg tcgagcacct gagcctgcat ctccctgctg
     1201 gtaagtgtgc ctgttttttc tataattctg tccgcgagtg tagatttacc gtgatcaata
     1261 tgggcaataa tacaaaaatt gcggattttg ctctgatcta ttgacatatg tcctcctaat
     1321 actttcaata actcatcaaa gtttatcaga tttgtgaaat aaagtctata ttattttaac
     1381 actttatcaa gtatatctgc aagtggttcc attgcattgt atgcctcctc cttggtattt
     1441 gtctgcgctc ccacctccac gagcagcgat ttcggacaca gatgcatatt atatctgtag
     1501 ccctttaagt atatgcgcct tgcaagcccc ggataatact catttgccat gagctgcagc
     1561 tgaaatgaaa atgccagatt gtcccccaga tactcattgg gcagactatt aatcggaccc
     1621 cttgcagcag tgcggcacag cccgttaaaa aacataatct gtgccatagg ctttccatct
     1681 attgtggcag caagccttgt agtctcagcc actccatccc tgtgcaggtc aataaccacc
     1741 tttatagacg gatttttctc aataatctct gtaatctcag gaagtgaatt ggaatacgca
     1801 tggtcgcggt cattcacatc atactgcccc ttgtgatgca gcacattgta gccgtatttt
     1861 tccctcaaaa gtgattcaag cctgtcaccc actccaacta cggtctctgt ctctgatgta
     1921 tcaagggaat cactgtaccc ctcctgcgaa tgtgtatggt atatgagtat atcaggaccg
     1981 ttgcctgact tttctataga catatcgcgc ccaagcaaag cctcctcaca cagctcatcg
     2041 gctcctatat atgtagtctt gtccacctga taatagtttt ttacaagatt gtcaaagctc
     2101 atatctacct gtggcatttt cacgttctgc tgctcctcaa gttctgccag ctcaccgttt
     2161 ttttcagctt catcaacaaa tgccagctgg tcggtatccg gcagcatatt gttcctgaca
     2221 cttgcatttt cattttccat cgcagcaagc tcctcatatg agagaatacc tcctgctgta
     2281 tcctcatcct ccacaatacg cagctccttc ttttttgaat catcacactc cttgtcagaa
     2341 tatgtaaggt gcggaaattt ttcttccacg aacttctttc ccacaccatc accgcaaaac
     2401 agcatagacg caatatatat aaatgctata agcacaatca tttttttctt cacaatatca
     2461 tctcccatct aaagcatcag gcagcccctg ctacataaag cttatgcggc tggcgctaat
     2521 gatattcgaa gcaataaaat tattcactat agtcatcata ttcatcgttt gaaaaagcca
     2581 tatttatgcc ctcagacaaa agccctgcca gtctcttaac cgtctcgtca atatccttcg
     2641 gcgtcacaaa catgccgttt aacttaggtg acatgagctt tgcggtgttc tcccggctgg
     2701 catttaccgc atctgaaaca atggtcaccg catcaatgac tgtcggaata ccaattgcga
     2761 taacaggcac tccgagattg tcatggttaa taccgtccct gtggtttcct accccgcttc
     2821 cgggcactat gcctgtatcc gtcagctgta ttgtacagcc cagcctcttt gtactgcgtg
     2881 ccgcaagtgc atcaattgtc acaacaaaat caggtttcac ctcgctcacc acacccttta
     2941 ttatctcaag gcactccatg cctgtctgag ccatcacgcc aggcacgagg ccgcacacac
     3001 tgtttacatt ctcgttgcta aacgcatacc tgccatattc ctttactata tggcgggtga
     3061 taaacaagct gtcaacgcat cttggcccaa gcgcatcagg agttacattt ctgtttccaa
     3121 gcccgacaac aagtgccgca tatatacgct tgtcacagac agcctttaca aagctcttaa
     3181 gctctcttga aagctccctt gtcacagcct gacagcactc ctcatctccc tcggacagca
     3241 tagcagcctc aattgtcaca tattttcctt taggacggcc tgtagccctg actgcatttt
     3301 ctgagtcaat tgtcactaca ctgactatga ttccattatc cagctttttt tcagagaaat
     3361 tcacacctct catatctgtg tttgcctttt taaggctctc ctgcgtctca agcgccagat
     3421 cagttcttgc acaccattta tcagccgaca aatcaataca cctcccctgt atgatattcc
     3481 aaaatcacat atatgttaat tttttgcaat ttttttcaaa atatgtattg acagagcaga
     3541 aaggttgaca tattatattg tagtatgttt gtgccaaagc gtccgtgtct gttttggcat
     3601 acattagatt aaatatttgg aggtgtacga gttggctaac attaaatcag caaaaaagag
     3661 aattttagta acagaaacta aagctgcaag aaataaagca atcaaatcag gagttaagac
     3721 agcaatcaag aaggttgagg ctgctgtagc tgcaaatgat aaggctgctg ctgaggctgc
     3781 attaaaggat gcaacttctg ttatcgcaag cgctggttca aagggtatct tccacaagaa
     3841 caacgcagct agaaaagtat ctcgtttaac aaagcttgtt aacggattag cttaattact
     3901 atatagcaat aaataataaa cccatcggtg ccgtcatacc nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggtcatatc tgcagagtac tgcactataa
     4021 aaagctccac agccatctgg tcattcatcc tgccggtctt cacatcctcc tcatatgcca
     4081 caccgtctct cacagccatc tttaaatcat ccagcgaata tgccctgcac tgcgcctgat
     4141 atttgcgcac cgcccactcc ggacatccgg cctttgatgc tatatccttg ttgccaaagc
     4201 ccttatttgt catggctttc acattcatca gtatattgaa ctggcgcgat ataagataca
     4261 taatacgcat ggccggttct ttaagtgcca gcagatcgta atacagctca agcgcacgct
     4321 tttgtttgtg tgatgcaatc gcatcaacca tctcaaacac cttgtttgtg atctgctctg
     4381 tcgttacagc ctcaacatcc gaaagctcaa tcacgtcctt gtccatgcag tagcacagca
     4441 gcttctcaag ctccttgtcg atattttcca tatctgtgcc ggttttttct ataaagctgt
     4501 tgtaggcagc ctggctcatg ccctttccat cttttctaat cctcattcct atccatctgg
     4561 caagtgtctc atccgtctgt tccttgaact ccacaggatt accgcacttg gctgccgcct
     4621 tataaagctt tgagcgctta tcaacgctct cctcaacaaa tataaagtgc gtgctctcag
     4681 gtgaatcctt taaatactct ccaagctcat cgcctgcctt tgaaaaaatg cctgcatcct
     4741 ctataagaat cactctttta tccgcaaaaa acggaagtgt ctccgccagg tcaattatct
     4801 ccttctggtt gattccatcg ccctcaaacg atgaaaagtt catgctgtcg ccctcactga
     4861 ccatagcctt tatcagcttg tccctgtact gttttatgag gtatctctcc tgtccataca
     4921 gaagataaac ctttttgaaa tctcctgctt ttatatcatt gtctattgtt ttcatatata
     4981 gcactctctt tatacttaat ctgaccgctt tgcattgtgt ataaaatacg gcactttgca
     5041 tccataagcc tatccatggc ttcatcagcc ggatgtccat agcggttttc tgccgcacat
     5101 gatactgcag cccaccttgg gcttaaagcc tcaatccagt ctgcagagct tgaatacctg
     5161 gaaccatgat gatttgcctt gtaaaaatcc acagaaagct catccccata ctcattaaca
     5221 agcttctttt ctacctcaga tgatatatct ccgccgaaaa taccactaaa cctgccatcc
     5281 gtgagcttca gcacaagaca catatcgttt ctgtctaaag cagtatcata aggccccgga
     5341 tacaggcaca tgataccatc agcttcttca tttgcagtac tgcgcgaacc tgatgcatca
     5401 tttatttcaa tcgaatcccc ctttgacata aggcatatat caattcctgc ttccttagct
     5461 ttaaaaagca gctcctccgt agcttcctct ttagccgctg cttctgccac aactatatgc
     5521 tctatagtat acccactgtc tatcacctca gccaatccgc ttatatgatc actgtctgca
     5581 tgtgaaacaa accagtatga tatactctgt atgccgttat acttaagaaa cggcataatc
     5641 acattctcgc caagctgttt tcggtcagag ctacctccat caataaagat gcatatgcct
     5701 gactcaaagc ggtaaaatat accatctccc tgccccacat caagtatatc aagctgcgca
     5761 gcagtttttg gcttcacaag caaaattgca agcaggactg ctgtacacac cactctttgg
     5821 acactgtaaa ggatttggcc tttttctttt gaaccacact ctatggcacg ttttctaagc
     5881 gaaaatgcca ccaaaaagcc actcataaca gcataaaaaa ggaaaatcct gtatccatca
     5941 ggagctcctg tgataaccga tgcccccgga agcttcataa caagaccgca aagcacctca
     6001 tagagcttta acaccgcaaa tgcaggaaac accagcagtt tgccgggcat cacgccaaag
     6061 cagcctgcag cagacgcaag cagacccgaa ataaatacga caggcaaagc cggtatcaca
     6121 atgagattta caaacactgc aaaaagaggc agctcataat agttcatagc cacaaccgga
     6181 agcatcggaa gctgtatgga tactgatgcc cagaattttc cgcccttagg agcactaatc
     6241 acaggcgtca caatacctat agcaagtatt gcccctaccg agaacacaaa tccggcatat
     6301 tccaaaacca taggattgtc ccacagcagg taaatgacca tgatgccgag agaattgagc
     6361 atatctgtgc atctgcctaa agctgccgca agcattgtaa gtatgaacat ccccacggca
     6421 cgccttgtgg acacggcatt tcctgtcata acagcatagc ttagtataag tgccgcagaa
     6481 cagacaaatg ccgctgtata gctcaccctt ctttttctga gaagcttgta aaaggccatg
     6541 ccaactatag aaatatgaag tccggaaata gccagtatat gtgaaatacc actgacctgg
     6601 tataaatcct ttatctcatc atccagatac gacttgtctc caagcagcat ggacgaaagc
     6661 actcctgccg ttgtctcatc agtcacctca agaagtgaag ctgacagcgt atcacgaagt
     6721 ttttcaagtc tgtggtaata ccatgcatgt ttaccggcgc ttgtcttaat actgtctgca
     6781 ttcaccgaga aatcaattcc ctgcgaacgg taaaagcgcg ctctgtcaaa ctctccctca
     6841 ttggttgcag acttaaacaa gcctatcttg ccgcaagctt tcatataatc gcccagatgt
     6901 gcgcttttgc tgtcacagta taccacgaca tcaccacagg atatttttct gccaaaagcc
     6961 ttgccgtacc caggctgtac tacgcagtcc gtcatataat acctgtatga gaaatacttg
     7021 tattcagttt tataaatctt cccccagacg atgacagatt tttcatctgt catatactgc
     7081 atatagctgt cacggttttc atccactgaa gcgtatctga attgtgctgt tgcaaaaaca
     7141 gctgacgata tcaggataag caaaacaact atgcgcctga caccggaaaa actgccctta
     7201 gacgccacac actcagctat taaaatcaca agcagtatgg caaaggtcaa aaaaccaaag
     7261 cccctgccat acatacctga aacagttcct gcagtataga ccaacgcaaa taaaaacatc
     7321 ggtcttttac taatactctt ctcctcctaa tccaatctat tctatttttc agttttttcc
     7381 gtggaatcat cagaatccac tatatcctta ttaaactcat acatgtcgct tagcttgtaa
     7441 ttataaagca gcatgccgtc attctcaccg tcaagcgaga tctgtacctt gtccacagta
     7501 ggaagtgagg tgagtgaatt aactatcgaa tacagcacca catcctctgt cagcttattg
     7561 tcaatagtat ttctgaatga agagtcaaag ttcagatagc atattctatc tgccacagac
     7621 acattgataa gctttgttgt gcccggaaga gttgccttga gcttagagcc cttcggtccc
     7681 tcaataagct gctcaacaac
//
LOCUS       DPUF01000085            2535 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_27433, whole
            genome shotgun sequence.
ACCESSION   DPUF01000085 DPUF01000000
VERSION     DPUF01000085.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_27433"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="DHV81_03085"
     CDS             <1..238
                     /locus_tag="DHV81_03085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93500.1"
                     /translation="SATRHLISSRRLLSHFHRRFELYLKQATEYIAADFTSRIKDKGV
                     TISFVRPSFEQYQHIIKMYIRKARGTKWHILLLQ"
     gene            214..849
                     /locus_tag="DHV81_03090"
     CDS             214..849
                     /locus_tag="DHV81_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HCI93501.1"
                     /translation="MAHLVITIGCEYGAKGNQIGKKVAKDLGIKFYDRDTVDEIIKEV
                     GIPKDIMEKVEEGITIAGKGAEGDVRGSFSKYADLTERAIHVQKTIIRKLSDRESCVI
                     IGRSADYILKEHKPILRIFIYSPDEVRIENVMKSHNLSEDDAKLFITEKDKRYHKRHM
                     ALTGSNRGDRHNRDMLIDSSLLGVDGTAELIESLARKVVENESKTEAGGDK"
     gene            849..2372
                     /locus_tag="DHV81_03095"
     CDS             849..2372
                     /locus_tag="DHV81_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI93502.1"
                     /translation="MEQKKSEFNKVLNAWDVLVIAFGAMIGWGWVVSTGGWIEKGGVL
                     GAAIGFVIGGIMIFFVGMTYAELTAAMPQCGGEHVFSYKAMGSTGSFICTWMIILGYV
                     SVACFEACAFPTIITYLWPGFLKGYLYTVAGFDIYASWLIVAIVVAFLIMMINIIGAK
                     TAAILQTVLTCIIGGAGILLIIASVINGTVDNLDGQMFASSSAGLNVKAIIGVAALSP
                     FYFIGFDVIPQAAEEINVPAKKIGSILILSVVLAVVFYALVIVAVGLVMGPQAIVDSE
                     QATGLVTADAMAAAFNTKIMAKVIIVGGMCGIVTSWNSFMLGGSRAMYSMAESYMIPK
                     FFAKLHPKHKTPINALILIGSLTMLAPFAGRKMLVWISDAGNFGCCVAYCMVALSFII
                     LRKKKPDMPRPYKVPAYRFFGTMAVIMSGFMVAMYCIPGSGGTLIAQEWGIVLAWCAL
                     GVVFFVFCKKKYKESFGTLVELISDEDAATLMPEADEVELDKVIDAAIDRVLAKKAS"
     gene            2396..>2535
                     /locus_tag="DHV81_03100"
     CDS             2396..>2535
                     /locus_tag="DHV81_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent alcohol dehydrogenase"
                     /protein_id="HCI93503.1"
                     /translation="METFNFSVPQDITVGKGSLSKLPEIAKKLGGKHALIISGPHLEK
                     MGL"
BASE COUNT          733 a          470 c          662 g          670 t
ORIGIN      
        1 gagtgcgaca aggcatttga tatcatcaag gagactgttg agtcactttc atagaaggtt
       61 tgaattatat ttaaagcagg caacagaata tattgcggca gattttacga gccggataaa
      121 ggacaagggc gtcacgatta gcttcgtgcg cccttctttt gaacaatatc aacatattat
      181 aaagatgtat ataagaaaag cgaggggaac gaaatggcac atcttgttat tacaatagga
      241 tgcgaatatg gcgctaaagg aaaccagatt ggcaaaaagg ttgctaaaga cctgggaatc
      301 aaattttatg acagggatac ggtagatgaa atcataaaag aggtcggtat tccaaaggat
      361 atcatggaaa aggttgagga aggtatcacc atcgccggaa aaggtgcaga gggtgatgtc
      421 agaggctctt tctccaaata tgcagacctc acagagcgtg caattcatgt ccagaaaact
      481 ataatcagaa aattatcaga ccgtgagtcg tgtgtgatta tcggtcgttc agcagactat
      541 atactaaagg aacataaacc gattttacgt attttcatat attcaccgga tgaagtgagg
      601 attgaaaacg taatgaagag ccacaatttg tcggaggatg atgcaaagct ttttatcact
      661 gaaaaggata agcgttatca taagcgtcac atggcgctga caggaagcaa ccgaggcgac
      721 agacacaaca gggatatgct tatagacagc agcctgttag gagtggatgg cacagcggag
      781 cttattgagt ctttggcaag aaaagtggta gagaatgaaa gtaagacaga agctggaggt
      841 gacaagtaat ggaacagaaa aaatcagaat ttaacaaagt cctgaatgcc tgggacgtac
      901 ttgtaattgc atttggagcc atgattggct ggggctgggt tgtctcaacc ggaggctgga
      961 ttgaaaaggg cggagtgctc ggtgccgcaa tcggcttcgt aatcggcggt atcatgatat
     1021 ttttcgtggg aatgacctac gcggagctga cagcagccat gcctcagtgc ggtggcgagc
     1081 atgtattcag ctacaaggca atgggttcga caggttcatt catatgtacg tggatgatta
     1141 tccttggata tgtcagtgtg gcatgctttg aggcatgcgc tttcccaaca atcatcacat
     1201 atctgtggcc gggctttttg aagggctatc tgtatacggt agcaggcttt gatatctatg
     1261 cttcttggct tattgtggcg atagttgtgg catttttaat tatgatgata aacattatcg
     1321 gcgcaaagac agcagcgatt ttacagactg tgctcacatg tattattggt ggcgccggaa
     1381 ttttgttgat aattgcatcg gtaatcaatg gaacagttga taacctggac ggacagatgt
     1441 ttgcaagcag ctctgcaggt ctgaatgtaa aggcaattat cggagtggca gctctgtcac
     1501 cattttattt tatcggattt gacgtaatac ctcaggcggc tgaggagatc aatgttccgg
     1561 caaagaaaat cggctcaata cttatcttat cagtagttct ggcagttgtg ttctatgcac
     1621 ttgtcattgt tgcggtaggt cttgtcatgg gcccacaggc aattgttgat tcagagcagg
     1681 caacaggact tgtcacagca gatgcaatgg ctgccgcatt caacacaaag ataatggcaa
     1741 aggttataat agtcggcgga atgtgcggta tcgtaacctc atggaactca tttatgctcg
     1801 gtggctcgcg tgccatgtac tcaatggcag agtcatatat gattccaaag ttttttgcaa
     1861 agcttcatcc aaagcacaag acacctatca atgcactgat tctcatcgga tctctcacaa
     1921 tgcttgcacc ttttgcaggc agaaagatgc tcgtatggat cagtgatgcc ggtaactttg
     1981 gctgctgtgt ggcatattgc atggttgctc tttcattcat tattcttcgc aagaagaagc
     2041 cggatatgcc aagaccttac aaggttcccg catacaggtt ctttggaacg atggcggtca
     2101 tcatgtcagg cttcatggtt gcaatgtact gcataccggg aagcggcgga acacttatcg
     2161 cccaggagtg gggcatcgtg cttgcatggt gtgcacttgg cgtggtattc ttcgtcttct
     2221 gtaaaaagaa atacaaggag tcatttggta cacttgttga gctcatctca gatgaggatg
     2281 ctgcaacact tatgccggag gcagatgagg ttgagctgga taaggttatc gatgctgcaa
     2341 tagacagggt acttgcaaag aaagcaagct aggaaacaga aaggacagat tatatatgga
     2401 aacatttaat ttttcggtgc cacaggacat cacagtggga aagggcagtc tttcaaagct
     2461 gccggaaata gccaaaaagc ttggaggtaa gcatgcgctc atcatttcag gacctcatct
     2521 tgagaagatg ggact
//
LOCUS       DPUF01000086            2664 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_19647, whole
            genome shotgun sequence.
ACCESSION   DPUF01000086 DPUF01000000
VERSION     DPUF01000086.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_19647"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="DHV81_03105"
     CDS             complement(<1..790)
                     /locus_tag="DHV81_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCI93504.1"
                     /translation="MKKSYGVNELRRMYLEFFESKGHLAMKSFSLVPHNDNSLLLINA
                     GMAPLKPYFTGQEVPPRHRVTTCQKCVRTGDIENVGKTARHLTFFEMLGNFSFGDYFK
                     HEAIAWSWEFLTEVLGLEKDRLYPSIYGEDDEAFDIWTKEVGVPGDRITRFYRDPKTG
                     ECDNFWEHGAGPCGPCSEIYYDRGEKYGCGKPDCKVGCDCDRFMEVWNNVFTQFNGDG
                     HGGYTELEHKNIDTGMGLERLAVVMQDVDSVFDIDTMKAIRDKVC"
     gene            complement(988..1746)
                     /locus_tag="DHV81_03110"
     CDS             complement(988..1746)
                     /locus_tag="DHV81_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCI93505.1"
                     /translation="MQIIFIHHSCFLVELDDKVLIFDYFDGDKVEGMHFTGKLPSYEP
                     DTKIYMFASHSHKDHYDMDILRLADKYSNIHYIFSKDIRISPHFLSKHGIDPAVRDRV
                     TFVSPDNTYHVDDLDIMTLRSTDAGVAFYVTSNGVSLFHAGDLNDWEWDGAGDLINGR
                     VRRAFRHEIKKLSEKPINVAFFPMDPKLMEYQFKGFDYFLQNTSAEFVFPMHMWQDYS
                     GITEYKKRLSNKDMADRVIEIERENQTFAFGENY"
     gene            complement(1867..>2664)
                     /locus_tag="DHV81_03115"
     CDS             complement(1867..>2664)
                     /locus_tag="DHV81_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HCI93506.1"
                     /translation="MEKLLYEFAVTRVDYDIPKWVQLLPYDNYVKQAVITYAKTFLAR
                     ASKLKDVALMSTDMPESDGDILKVVSLAGMGLSDGVVKVKIEIAEKYYYENISTLCGV
                     TVSGEKELISLILSLTEEKNEYEKIKDAFSSVQQKGYGVVMPQLSDIRMEEPVLIAHG
                     NKFGVKMKAISPSIHMIRANIETEIAPIVGSREQAEDLIDYIKNGENSDSGVWKTNIF
                     GKSVGELMEDGIRSKIMQMDDECQIKLQETMQKIVNDSNGGMICIII"
BASE COUNT          746 a          689 c          431 g          798 t
ORIGIN      
        1 cgcagacctt gtcacggata gccttcattg tatcaatatc gaataccgaa tcaacatcct
       61 gcattacaac agcaagacgc tcaagtccca tacctgtatc aatgttcttg tgctcaagct
      121 cagtgtatcc accgtgtccg tcaccgttaa actgtgtgaa tacattgttc cacacctcca
      181 tgaaacggtc gcagtcacag cctaccttac aatcaggctt tccacagccg tacttctcac
      241 cgcggtcgta gtaaatctca gagcaaggac cgcaaggacc tgcgccatgc tcccagaagt
      301 tgtcacactc accggtcttt ggatctctgt agaaacgtgt aatacgatct cccggcactc
      361 ctacttcctt agtccagata tcaaatgcct catcatcctc accgtagatg gaaggataaa
      421 gcctgtcctt ttcaagaccg agcacctctg tcaaaaactc ccatgaccat gcgattgctt
      481 catgcttgaa ataatcgcca aacgagaagt ttccgagcat ctcaaaaaat gtaagatgac
      541 gagctgtctt acctacattc tcgatatcac ctgttctgac acacttctgg catgtagtca
      601 cacggtgtct tggcggaacc tcctgtcctg taaaatatgg tttaagcggt gccatacctg
      661 cattaataag aagcagactg ttgtcattgt gaggcaccag tgaaaaactc ttcattgcga
      721 gatgtccctt gctctcaaag aactcaaggt acatacgacg taattcgtta acgccataac
      781 tcttcttcat tacttgaatc tcctattctt ttgtctttgc gtccttcatt ttacgaagct
      841 ttacccctat taaagcccca caaaaaccta tggccgccaa aacaatcata ataataacat
      901 attgtgctac gcacatgaaa aagttcccca taattaccct cctaaattta ttgaactaat
      961 ttattataaa tgaatttgtg aaataattca atagttctct ccaaaagcga acgtctgatt
     1021 ttcacgttca atctcaatca ccctgtcagc catgtcctta ttgctcagcc tctttttgta
     1081 ttcagtaata ccgctgtagt cctgccacat gtgcataggg aaaacaaact ctgccgaggt
     1141 attttgtaaa aaataatcaa aacccttgaa ctgatactcc ataagctttg gatccatcgg
     1201 gaaaaaagcc acgtttattg gtttttccga aagctttttt atctcatgcc tgaaggctct
     1261 gcgcactctg ccgtttatca ggtctcctgc gccatcccac tcccagtcgt ttaaatcacc
     1321 ggcatgaaac aggctcactc cgttagaagt gacgtaaaac gccacaccgg catcggtgga
     1381 tctaagtgtc attatatcca gatcatccac atgatatgta ttgtcaggtg aaacaaatgt
     1441 caccctgtcc ctgacagcgg gatcaatgcc atgctttgac aaaaaatgcg gactgatacg
     1501 gatatccttt gaaaaaatat aatgtatatt cgaatatttg tcagccagac gcaaaatatc
     1561 catatcataa tgatccttgt ggctgtgact tgcaaacata tagatttttg tatccggctc
     1621 ataagatggc agctttccgg taaaatgcat accctccacc ttgtctccat caaaataatc
     1681 aaatataagc accttgtcat ccagttccac aagaaaacaa ctgtgatgta taaaaattat
     1741 ctgcatgtaa cctactctct ttcgctttta aatcagtctt tatgtatcat tatgctatct
     1801 ttatgctata tagtcaactt atatttcgta taataagggt caaaatacct tttgaccctc
     1861 atatcattat ataattatac aaatcatgcc gccatttgag tcattcacaa tcttttgcat
     1921 ggtttcctga agctttatct ggcactcatc atccatctgc attattttgc ttcttattcc
     1981 gtcctccata agctcaccga cagactttcc aaaaatatta gtcttccaga ctccactgtc
     2041 actgttttca ccatttttga tgtaatctat cagatcctct gcctgttccc tgcttccgac
     2101 tatcggcgct atctcggtct cgatattagc tcttatcata tgtattgacg gtgaaatagc
     2161 cttcattttt acgccaaact tgtttccatg ggctataagc accggctcct ccattctgat
     2221 atcagacagc tgtggcatca caacaccata acccttctgc tgcaccgatg aaaaagcatc
     2281 cttaatcttt tcgtactcat tcttttcctc agtgagtgaa agtatcaggg aaatcagctc
     2341 cttttccccg gaaacagtca caccgcacag cgtggaaata ttctcgtagt agtacttctc
     2401 tgcaatctca attttgacct taaccacacc atcagacagc cccatcccgg caagagatac
     2461 cacttttaat atatccccat ccgattcagg catatcagtg ctcatcagtg cgacatcctt
     2521 cagctttgaa gcccttgcaa gaaaagtctt tgcatatgta attacggcct gctttacata
     2581 attgtcataa ggcagaagct gcacccattt cggtatatca tagtccactc tcgtgactgc
     2641 aaattcatat aagagctttt ccat
//
LOCUS       DPUF01000087            5057 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7194, whole
            genome shotgun sequence.
ACCESSION   DPUF01000087 DPUF01000000
VERSION     DPUF01000087.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7194"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /locus_tag="DHV81_03120"
     CDS             complement(<1..1947)
                     /locus_tag="DHV81_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004849897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monosaccharide ABC transporter substrate-binding
                     protein, CUT2 family"
                     /protein_id="HCI93507.1"
                     /translation="MAKKVTIKDIADMAGVSIATVSHVINRTRYVSPDLVEKIENIII
                     ETGYHKKIEEKERRLKVGRQSEIVAVIPNVESTIYRYMVGYLKKYVSIQGYQFYIAIT
                     DNDIKEEAQVLEGLIQNKKTAGIIHVPVSDVAADYKKLIESGIPFVCMERNILGDGID
                     SVEFRDREAIFKGTDYLLQCGHKNILFLRESLKSTTRDERTRGFLLALEEHGINTNDA
                     NISDINIESGADNCILEIQKAMRRYMPTAIMAGGNRITLYLMKTMRDRGIECPEEISV
                     VGFGDEGWSELTYPPLTILKRDVEGLSRRAVNMLFEKINTGHVIEQDYYADVELIIRK
                     STRMLDNGPFGEEAATPESIVITKEEKSRLRAGNFRVAISFHYTGTSWAELHEKGIRD
                     ELEQYGIDIISVTDAHFDASLQNAQLEGIRLQKPDAVIAIPADDKETKEQFRELAKVS
                     KLVFLSNVPENMEKNSYVCCVSVNEIENGINVGRMMGQYCKGKHVEAGFIIHGAVFYG
                     TRARDEAAEKIISEQYKNIDIKAIRGFGEIENAYQVCKDMITENPQIKVLYVSWDRPA
                     LLAIKALKEMHREDIAIFTTDLDYKIAQYMESGIVKGLSTQRPYEQGRTAAHVVAKSL
                     LSNDVPKYVGVQPYVVDAKQLGRAW"
     gene            complement(2207..3391)
                     /locus_tag="DHV81_03125"
     CDS             complement(2207..3391)
                     /locus_tag="DHV81_03125"
                     /inference="COORDINATES: protein motif:HMM:PF00990.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93508.1"
                     /translation="MVITIIVIEEILMANGLTVLMMCYLLSCRRKNRESLHTEDKVYD
                     GIAKVNILGAVAETISFLVDGKSFIGCRQLNYISNSLCFIGTVSMGLLWCLYVNLRIY
                     RNFKKISEKMAVMMIPWMIEVVMVLGNLIKPGIMFKVSADNVYQRTGGALAGYITLVI
                     YLAYSLYMVYHSRKQGVNLSYFPVFYFVCICFAGVLIQLVFYGVTSSWLITAVTLIFT
                     QMQSYAENIYMDELSGLYNRRYLKAVLAKRKNTDANSLYGIMMDVNDFKYINDSFGHS
                     MGDKAISTMGNILFKSIPDAGIAIRYAGDEFIVLLTDADKACVLATINEINKNISRFN
                     DSKAEPFELSVSMGYAEFGKDDNAEVFLRNMDDKMYEEKRRYHGAAPSIPELNEVYDF
                     GR"
     assembly_gap    3471..3577
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3670..4131
                     /locus_tag="DHV81_03130"
     CDS             3670..4131
                     /locus_tag="DHV81_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HCI93509.1"
                     /translation="MKITTLCYIEHDGQYLMLHRIKKENDINEGKWIGVGGHAENGES
                     PEECLLREVKEETGLTLTSYRFRALITFISDKQEPALMCLFTADEFSGKLITCNEGDL
                     KWVDKTIVPTLPTWEGDAIFLKLLLENEENFFTLKLVYEGESLVDQKLEIY"
     gene            complement(4134..4517)
                     /locus_tag="DHV81_03135"
     CDS             complement(4134..4517)
                     /locus_tag="DHV81_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCI93510.1"
                     /translation="MKVLNIEDDTFKHNDICKALSGCGIKDVEWSNNLADGWKRIKNS
                     MDSNNPYDLIITDMYYPNEPGGREEESGDVLIDRMIKNKITIPVILCSSVNLKYPEIY
                     GCVYYSRERNWEAGMQKLVNSLVAG"
     gene            complement(4492..4779)
                     /locus_tag="DHV81_03140"
     CDS             complement(4492..4779)
                     /locus_tag="DHV81_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93511.1"
                     /translation="MELYDILLRRGYPEPFCDEITKNLNTDWTAQRMIGYLSHYKKLP
                     MEEIADEMLAILNDRNRIMRKHELEETNARWNEYLNRKSQEDSHESTEYRR"
BASE COUNT         1408 a         1177 c          681 g         1684 t
ORIGIN      
        1 ccatgccctt cctagctgct tagcatccac aacatacggc tgcactccaa catatttcgg
       61 cacatcatta cttaataaag atttcgctac cacatgcgca gctgttcttc cctgctcgta
      121 aggtctttga gtacttaatc cctttacaat accactttcc atatattgtg caattttata
      181 atctaaatca gttgtaaata tagcgatgtc ttctctatgc atctctttca acgccttaat
      241 tgccaacagc gccggtctat cccagctgac atacaagact ttaatttgtg gattctcagt
      301 aatcatatct ttacaaacct gatatgcatt ttcaatttct ccaaaacctc ttattgcctt
      361 aatatctata tttttgtatt gttctgaaat aattttttcc gcagcctcat ccctggcacg
      421 cgtaccataa aagaccgctc catgtatgat aaatccggct tcaacgtgtt ttcctttaca
      481 gtactgcccc atcatcctgc cgacattaat tccattttca atctcattaa cagacacaca
      541 gcaaacataa ctattttttt ccatattctc agggacatta ctcaggaata caagctttga
      601 cacctttgcc aattcacgga attgttcttt tgtttcttta tcatcagccg gaattgcaat
      661 tacagcatcc ggtttctgta aacgaatgcc ttcaagctgt gcattttgca aagatgcatc
      721 aaaatgtgca tctgttactg atataatatc aattccgtat tgttccaact catcgcggat
      781 tcccttttcg tgtaattccg cccatgatgt tcctgtataa tgaaacgata ttgccacacg
      841 gaaatttccg gcacgcaaac ggctcttttc ctcttttgtt atgactattg attccggtgt
      901 agctgcttct tctccaaaag gtccattatc caacattctt gtggattttc ttattatcaa
      961 ctctacatcg gcataataat cctgttcaat cacatgtcct gtatttatct tttcaaaaag
     1021 catatttact gctcgtctgc tcagcccttc aacatctctc tttaaaatcg tcaatggtgg
     1081 atacgttaac tccgaccatc cctcatcacc aaatccaaca accgatatct cttcaggaca
     1141 ttcaattcct ctatccctca tagttttcat aagatataaa gtaattctat ttcctcctgc
     1201 cataattgca gttggcatat atcgtctcat tgccttttgt atttccaaaa tacagttatc
     1261 agctccggat tcgatattaa tgtctgaaat atttgcatca tttgtattga ttccatgttc
     1321 ttctaatgca aggagaaagc ctcgtgttct ttcatctctc gtcgtactct tcaagctttc
     1381 tcttaaaaac aaaatattct tatgaccaca ttgtaacaga taatcagttc ccttgaaaat
     1441 ggcttctcta tccctaaatt caacagagtc tataccatct cccaaaatat ttctttccat
     1501 gcagacaaat ggaattccag attcaatcag ctttttataa tctgccgcaa catcactgac
     1561 tggtacatgt ataatccctg ctgttttttt attctgtatc agtccttcca gtacctgagc
     1621 ctcttcttta atatcattgt ccgtaattgc aatataaaac tgataaccct gaatgctaac
     1681 atattttttt aaatatccca ccatataacg ataaatggtg ctttcaacat ttggaataac
     1741 tgcaactatt tccgactgcc ttccaacctt gagtcgtctt tctttttctt ctattttttt
     1801 atgatatccg gtttctataa taatattttc tattttttca acaaggtccg gactcacata
     1861 tcttgttctg tttataacat gcgaaacggt tgcgattgaa acccctgcca tatcagctat
     1921 atcttttata gtaacctttt ttgccataat gatattccct cttcttttaa aatttatatc
     1981 gtataattgt tatatacatt cccttatatt cccatagttc tattgcattt gataatgtcc
     2041 tgactttact ataagcatca ttttacacca tatctactgt atttagtagt ccaacttcat
     2101 acagattttt atatttttta taaattcatt gcttataata cagaaaaaga ccttgagttc
     2161 attataaact caaggtcttt cgtgctcccc cataatacca tagtcattat cggccaaaat
     2221 catatacctc attcaattca ggtatagatg gtgctgctcc atgatatctg cgtttttctt
     2281 catacatttt atcatccata tttctcagaa atacttctgc attatcatct ttgccaaatt
     2341 ccgcatatcc catagaaaca ctcagctcaa atggctctgc ctttgagtca ttaaaccttg
     2401 aaatgttttt attaatctca tttattgtag ccaatacaca tgctttatct gcatctgtca
     2461 gcaatacaat gaactcatct cccgcatacc ggattgcaat tcccgcatca ggtattgatt
     2521 taaaaagtat atttcccatt gtgctgatag ccttgtcacc catactatgt ccaaagctgt
     2581 cattgatgta cttgaaatca tttacatcca tcataattcc atacaaagag tttgcgtctg
     2641 tattttttct ttttgcaagc acagccttaa gatacctgcg attgtacaat cccgaaagct
     2701 cgtccatata tatattttcc gcatacgact gcatctgtgt aaatatcaac gtaacagcag
     2761 taataagcca tgatgatgta acaccataaa acacaagctg tatgagtact ccggcaaaac
     2821 aaatacaaac aaaataaaac actggaaaat agctcagatt aactccctgc tttcgcgaat
     2881 gatacaccat atacaagctg taagccaaat atattacaag tgtaatatag cctgccagtg
     2941 cacctcctgt cctctgatat acattatccg ctgatacctt aaacataatc cccggtttga
     3001 ttagattgcc taacaccata acaacctcta tcatccacgg aatcatcata actgccattt
     3061 tttcagatat cttcttaaaa ttccgataaa tacgaagatt cacatataaa caccagagca
     3121 gccccatact gaccgtacca ataaaacaca ggctgttaga aatataattg agctgtctgc
     3181 atccaataaa gctctttcca tctacaagaa aacttattgt ctcagccaca gctcccaata
     3241 tattcacttt tgcaattcca tcatatactt tatcttccgt atgaagactc tctctgttct
     3301 ttcgcctgca tgacaaaaga taacacatca tcagaactgt caatccattt gccattagaa
     3361 tttcttctat aactattatc gtaatcacca tccaaacatc tgtgaaattc aatcagctta
     3421 ctgttttatg ctttctgcca tatattccgc agtattgtaa gcctaacctc nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt ctttttatca ccaatatcca
     3601 atcttgatgt ttcaataaaa tgcggtttta tgataggatt atgttataat catctgacgg
     3661 aggtacatca tgaaaataac tactctatgc tacatagaac acgatggaca atatctcatg
     3721 cttcatcgta ttaagaaaga aaatgatata aatgaaggaa aatggattgg tgttggcggt
     3781 cacgccgaga atggtgaatc acccgaagaa tgcttgctcc gcgaagtcaa agaggaaacc
     3841 gggctcacac tcacatcata ccgtttccgc gcactcataa cttttataag tgataaacag
     3901 gagcctgcat tgatgtgtct tttcaccgct gatgaatttt ccggcaagct tataacatgc
     3961 aacgaaggtg acctcaaatg ggtggataaa actatcgtgc ccacacttcc tacctgggaa
     4021 ggcgatgcta tcttcttaaa gctcctgctt gaaaatgaag aaaatttctt cacacttaag
     4081 cttgtatatg aaggtgaatc actggttgat cagaagcttg agatttatta gtattatccg
     4141 gcaacaagac tatttacaag cttctgcata cctgcctccc agtttctttc cctgctataa
     4201 taaacacaac catatatttc cgggtatttg agattcacac tggaacagag aattacaggt
     4261 attgtgattt tgttcttaat cattctgtca atcagaacat ctcctgattc ttcttctctg
     4321 cctcccggct cgttcggata atacatatca gtaattatca gatcatacgg attattacta
     4381 tccattgagt ttttaatccg tttccaccca tccgcgagat tattggacca ttcgacatct
     4441 ttaatcccac atcctgaaag ggctttgcaa atatcattat gtttaaatgt atcatcttcg
     4501 atattcagta ctttcatgtg aatcctcctg actttttctg tttaagtatt cattccatct
     4561 tgcatttgtc tcctcaagct catgctttcg cattatgcgg ttgcggtcgt tcagtatcgc
     4621 aagcatttca tcagcgattt cttccatggg aagcttttta tagtgtgaca gatatcctat
     4681 catcctttga gctgtccagt cggtattaag attttttgtt atttcatcgc aaaacggctc
     4741 cggatacccg cgcctaagca atatatcata tagctccatg ctctttgctg tcctgggttt
     4801 catatagtgc ctctccaatt ttctcaatca atgtcacatt cgcgacagct actttacatc
     4861 tgcgtctaca aatatctgca ttcttatatc caattaaaat cgccacctgt cacagcaagc
     4921 gataacagct tatccatctt ctctacctct tcaacgcctc taaccatctg aataagcttc
     4981 gcagattcta caacttttgg catctcctca tcaggtccta tctccatatc tgcctcttct
     5041 ccacagtatg gacatac
//
LOCUS       DPUF01000088            7949 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7981, whole
            genome shotgun sequence.
ACCESSION   DPUF01000088 DPUF01000000
VERSION     DPUF01000088.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7981"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="DHV81_03145"
     CDS             complement(<1..647)
                     /locus_tag="DHV81_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCI93512.1"
                     /translation="MAKEKKLVEAITSMEEDFTQWYTDVVKKAELMDYSSVKGCMIFK
                     PNGYAIWENIQKNLDAMFKETGVENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTQGG
                     LETLQERLAVRPTSETLFCELFSKTVQSHRDLPKVYNQWCSVVRWEKTTRPFLRSSEF
                     LWQEGHTVHATAEEAEARTVQMLNIYADFCEKYLAIPMVKGRKTDKEKFAGAEAT"
     gene            complement(742..1887)
                     /locus_tag="DHV81_03150"
     CDS             complement(742..1887)
                     /locus_tag="DHV81_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCI93513.1"
                     /translation="MKVVVAIDSFKGSMTSMQAGMACKEGILAAIPKADVVVRPLADG
                     GEGTVTALVEGMNGTYEHVDVTGPMGQKVHATYGVLEDDTAVMEMAEASGITLVEGKP
                     DPWKATSLGVGEMILDAVHKGCRNFIIGIGGSATTEGGIGMLMALGYEFYDEDDNILP
                     PVFGSLARVRKIKADKVPSELKQCHFKIACDVTNPLCTEQGCVYIFGKQKGVQEHEKA
                     QMDKMMRQYADCVEEYAGKRFSETPGAGAAGGLGFAFLFGLENVELKSGIDIVLNAIQ
                     LDKELKNADIVVTGEGRLDGQSAMGKVPVGVAKAGRKNGCKVIALAGSVTDDAVACNK
                     EGIDAFFPIIRGATTLNEAMDIKNAQKNMKNTAEQVFRLIDISSLME"
     gene            complement(1917..2894)
                     /locus_tag="DHV81_03155"
     CDS             complement(1917..2894)
                     /locus_tag="DHV81_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HCI93514.1"
                     /translation="MKGKHKVIVSTKRLKYEFELRRNLTIIQGDSATGKTTLVDMIRD
                     FVNNPTGTPVEVICDKKCHVVEGSLWKEQLSGISDCIVFIDEGNEFITTVDFADKIQK
                     TDNYYVIVTREALPALPYSVEEIYGIRTSGRYGTLKQSYHEFYRIYGTDTYEDKVSPE
                     IIITEDSNSGYQFFEHVCIENGLKCESMNGKSNVFHYLNKHKGEKILVIADGAAFGSE
                     IDRVLQVIHGRKNVALYLPESFEWMIMDADILKNNTVRSILSNPSEYVESKLYFSWER
                     FFTAILIEQTKDTYLAYAKRKLNPAYLSGAIKESILRKMNIIDLNKKDE"
     gene            complement(2910..3347)
                     /locus_tag="DHV81_03160"
     CDS             complement(2910..3347)
                     /locus_tag="DHV81_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4869 domain-containing protein"
                     /protein_id="HCI93515.1"
                     /translation="MLNIFYGDMKEAVYNTAAYFKYDYEDNWITDPFVKKMIKDVDQS
                     IVLDSGVIDSPVLGKIPPTGLSGGVKTLILVKFEKNKIFNASTCGDNCAKWLLKIAET
                     EDRTINLRHLMKFEVEPFDIRILNTNEIVHTMEELVSIAGEFV"
     gene            3652..4353
                     /locus_tag="DHV81_03165"
     CDS             3652..4353
                     /locus_tag="DHV81_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HCI93516.1"
                     /translation="MKNYKRGLTAGIPIALGYLSVSFTFGIMAVSYGLSWWQAVLISM
                     TTVTSAGQFAGIGIMMHPGQYFQMLISQITINIRYSFMSISVGQKADSRFSGISRWIL
                     GFMMTDEIFAVASQEDSVSRSFFAGLSTLPYIGWAVGTLIGAILGSILPDRLMSALSL
                     AIYGMFVAIVVPEMKKSRAVVFVVLLALVLSCMFYYVPFLSRISSGITITVVAIVAAV
                     IGSLLFPVADDTDKE"
     gene            4398..4706
                     /locus_tag="DHV81_03170"
     CDS             4398..4706
                     /locus_tag="DHV81_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCI93517.1"
                     /translation="MKNSVFFIYLLILAGSTYLIRVIPFIAIKEKINNSFVRSFLYYI
                     PYAVLTAMTIPAVFYATNWWVGAAAGLIAAVIFALKGKGLTTVAVASCVAVFIVELFH
                     "
     gene            complement(4776..6182)
                     /locus_tag="DHV81_03175"
     CDS             complement(4776..6182)
                     /locus_tag="DHV81_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCI93518.1"
                     /translation="MVSVAVIVVALLLEAGILKYVNDANAYKLSKVLLDRVFTVLDKN
                     DQSEEELIESLKDDYIVRAKAVSYIVDAKPQVENDVEELQKIAKLMSVDEIHLFDETG
                     CITSGSVPKYFGYSFDSGTQMSYFKPMLTDKSLTMCQDVTPNTSEGKAMMYAITWNEA
                     GTMMVQVGLKPQRLLDELKQNEISTVVSDMPVYKGMEIYVADADTGLVKGATDCDKIG
                     KNFHDLGLPTDIKESDKPTVRQISVNDLGCRCVIRRGDKYIVAVTIDKSFYMTNSVVA
                     LLVVGIYLILASCCIMYMFSKIMKEKYEKEKLLYISNTDALTGCLNRHAYETDINKLD
                     LKKEWIYISLDLNSLKHINDTYGHDVGDEMICAAAACMMASFGEFGKVYRIGGDEFVV
                     IVTQKPDELDTMTKHFDSSVAEWRGKIVNSMTISYGCVRSLEEDWELVHDIEKEADRR
                     MYASKARYYSDSGIDRRK"
     gene            complement(6257..6445)
                     /locus_tag="DHV81_03180"
     CDS             complement(6257..6445)
                     /locus_tag="DHV81_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93519.1"
                     /translation="MKHRNAQPWTAGQGVMRHGRRIKAGFVKHRKTVSIISYRILAAQ
                     RSLRGVCALPRQAVTCGP"
     gene            complement(6559..6960)
                     /locus_tag="DHV81_03185"
     CDS             complement(6559..6960)
                     /locus_tag="DHV81_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HCI93520.1"
                     /translation="MYEMKPEYYIGIDMIDEEHKQLFKYADEAYELLHDEFTPDKYDK
                     MDIILEKLRDYTVKHFADEEQYMESIHYKKMFTQKVQHMEFVQKLDEFIEHHSDEEID
                     QDEQIMGILKYLTDWLINHILYVDCQIPKPQ"
     gene            complement(7156..7836)
                     /locus_tag="DHV81_03190"
     CDS             complement(7156..7836)
                     /locus_tag="DHV81_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCI93521.1"
                     /translation="MQNSKEKTLSQRIAERIIKYILEENLQPGDKLPNETVMCERLNV
                     GRSSLREAMRALASRNVITIRQGSGSYVSATPGMIDDPFGLTFVEDKQKMIKDLMEIR
                     FLIEPSIAAMAAIRADETDIKNILTACENTEKLLLANKNHAEKDIAFHTAIALSSKNI
                     VVPKLVPIINSSIPLIRESAKFTMRDETIEIHREIADAIAAHDAVRAHDAMYLHLIYN
                     RKHIKSIN"
BASE COUNT         2016 a         1876 c         1373 g         2684 t
ORIGIN      
        1 tatgtagcct ctgctccggc gaacttctcc ttgtctgtct ttcttccctt taccataggg
       61 attgcaaggt acttctcaca gaaatccgca tagatgttta acatctgtac tgtacgtgcc
      121 tctgcctcct cggctgttgc atgaacagta tgtccctcct gccataagaa ctctgatgag
      181 cggaggaatg gacgtgttgt cttctcccaa cgcactactg agcaccactg gttatatacc
      241 tttggaaggt ctctgtgtga ctgtactgtt ttgctgaaaa gctcgcagaa aagtgtctct
      301 gatgtaggtc ttactgcaag tctctcctga agtgtctcaa gtccgccctg tgtgacccat
      361 gcaacctcag gtgcaaagcc ctcaacatga tccttttctt tctggagcag gctctccgga
      421 atgaacattg gcatgtatac attctctaca cctgtctctt taaacattgc atcgagattt
      481 ttctgtatat tctcccagat ggcataacca ttgggcttaa agatcataca tccctttaca
      541 gatgaataat ccatcaactc tgctttcttc acaacgtctg tataccattg tgtgaaatct
      601 tcctccatgg aagtgatggc ctctactaat tttttctcct tagccattgc ttttctcctt
      661 ttttatttaa atattctatg atatacgggg cttttatatc tcatccggcc cctgcaccac
      721 gcataatttt actaacatat attattccat aagcgagctg atgtcaataa gcctgaaaac
      781 ttgttctgca gtgtttttca tatttttttg agcatttttg atatccatag cctcattaag
      841 cgttgttgcc cctcttataa tagggaaaaa cgcatctatg ccttctttat tacatgccac
      901 tgcatcgtca gtgacactcc ctgctagtgc gattacttta cagccgtttt ttctgcctgc
      961 ttttgccact ccgacaggca cctttcccat ggcgctctgt ccatcaagcc tgccctctcc
     1021 ggtcactacg atatcggcat ttttcagttc cttgtcaagc tgtattgcgt ttagcactat
     1081 atctatgccg gatttaagct ccacgttctc aagtccaaaa agaaacgcaa agccaagacc
     1141 tcccgcagcg cctgctcccg gtgtctcgct aaatcttttt cccgcatact cctcaacaca
     1201 gtctgcatac tgtctcatca ttttatccat ctgtgccttt tcatgctcct gtacgccctt
     1261 ttgctttcca aaaatgtata cgcagccctg ctctgtgcac agtggattag taacatcaca
     1321 tgctattttg aaatgacact gcttcaattc tgaagggacc ttatcagcct tgatctttct
     1381 tacccttgca agcgagccaa ataccggtgg aagaatattg tcatcctcat cataaaactc
     1441 atagccgagc gccatcagca ttcctatgcc gccctcagtg gttgcgcttc cgcctattcc
     1501 gattatgaaa ttccgacagc ccttgtgcac ggcatccaat atcatctctc ccacacccaa
     1561 tgaggtcgct ttccatggat caggtttacc ttcaacaagt gtgattcctg atgcttctgc
     1621 catttccata acagccgtat catcctcaag tactccatat gtcgcgtgca ccttttgtcc
     1681 catcggtcct gttacatcaa catgttcata tgtgccattc atgccctcta ccaaagcggt
     1741 tacagttccc tcgccaccat ctgcaagtgg tctgaccacc acatctgctt tcggtattgc
     1801 cgccaatatg ccttctttac aggccatccc ggcctgcatg cttgtcatgc ttcctttaaa
     1861 tgaatcaatt gctactacaa ccttcattta accattcctc tattttttct taatatttat
     1921 tcatctttct tattcagatc aattatgttc atttttctta aaatcgactc tttaattgct
     1981 ccacttaaat atgccggatt taatttacgt tttgcatatg ccaaataggt gtcttttgtt
     2041 tgttcaatca gaatagctgt aaaaaacctt tcccaactga aataaagctt gctctctaca
     2101 tactctgatg gattactcaa tatgcttctt actgtattat tctttaaaat atcggcatcc
     2161 ataatcatcc attcaaatga ttcaggcaaa tataaggcca cattttttct gccatggata
     2221 acctgcaaca ccctgtcaat ctcggaacca aatgccgccc catctgcaat gacaagtatt
     2281 ttttcacctt tatgtttgtt cagataatga aagacatttg attttccatt catagattca
     2341 catttcaaac cattttctat acatacatgt tcaaaaaatt gatatcctga attgctgtct
     2401 tccgtaataa taatttccgg actcacttta tcttcatatg tatctgttcc atatatccga
     2461 tagaattcat gataactttg tttcaatgtc ccatatcttc cagatgtacg aattccatag
     2521 atttcttcta cgctatatgg caatgctggt aaagcttccc tagtaacaat tacataataa
     2581 ttatctgttt tttgaatttt atcagcaaaa tctactgtcg ttatgaattc atttccctca
     2641 tcaataaata caatacagtc tgatatgcct gacaactgct ctttccataa acttccttca
     2701 accacatgac attttttatc acatatcacc tctacaggtg tgccggttgg attattcacg
     2761 aaatcacgaa tcatatctac aagtgtagtt tttccagttg cactgtctcc ctggataatc
     2821 gtcagatttc tccgaagttc aaattcatac tttaaacgtt ttgttgatac aataacttta
     2881 tgttttcctt tcatcgtatc ctcctgccat caaacaaatt cacctgcaat tgataccagt
     2941 tcttccatgg tatgtacaat ttcattcgtg ttaaggattc ggatgtcaaa cggttcaact
     3001 tcaaatttca tcaaatgacg taagtttatt gttctgtctt ctgtttctgc aatttttaat
     3061 aaccattttg cacaattatc tccacaggtc gatgcattga aaattttatt tttttcaaac
     3121 ttcaccaaaa tcaatgtttt aacaccacct gataaccccg taggcggtat ttttcctaac
     3181 actgggctgt cgattactcc actgtccaaa acaatcgact gatccacatc ctttatcatt
     3241 ttcttcacaa atggatctgt aatccagtta tcttcataat catatttaaa atacgctgcc
     3301 gtattatata ctgcttcttt catatctcca taaaatatat tcagcataca aaatctactc
     3361 ctcctgttag attagccttg aatgaatttc acctcactac aatacaaatg tattttccaa
     3421 aacctaggta agtgcttacc atatgaacct ccacgaaatc catcaatccg ctgtacttag
     3481 ctacagtata gcggattgat gggcaaatta caattattat taccggtctt tatgcagtta
     3541 catgatgcgc aactgcaaga ttgtaaagtg gtacaaacaa tttgaaagtg cattttcatg
     3601 aaatttgtgc tagaataaca tgttgttgta atatgctaag gagaatttag aatgaaaaac
     3661 tataaacgag gactgacagc gggaatcccg attgcgctgg gatatctgtc agtttcattt
     3721 acttttggaa ttatggcggt gtcatatgga ctatcctggt ggcaggctgt gctcatttcg
     3781 atgaccactg tcacatctgc cggacagttt gcaggcatcg gaattatgat gcatccggga
     3841 cagtatttcc agatgcttat ttcgcagatt actattaata taagatattc ctttatgtcg
     3901 atttcggttg gtcagaaggc tgattccaga tttagcggaa tttcaagatg gatactcggc
     3961 tttatgatga ctgatgaaat ttttgccgtg gcaagccagg aggattctgt gagcagaagc
     4021 ttttttgcag gactttcaac tcttccatac atcggctggg ctgtcggtac gctgataggt
     4081 gctatactgg gaagcattct gccggatagg cttatgagtg cgctgagtct tgctatttat
     4141 ggcatgtttg tggctatcgt ggttcctgag atgaagaaat cgcgtgcggt cgtgtttgtt
     4201 gtgctgcttg cactcgtgct tagctgcatg ttttactacg taccgttttt gtcaaggatt
     4261 tcaagcggta tcaccatcac tgttgtagcc attgtggctg cagttatcgg ttcgctgctg
     4321 tttccggttg cagatgatac tgataaggaa tgatttatta aatatttgat aataagcgta
     4381 taaggagatt ttacataatg aaaaacagtg tattttttat atatttactc atactcgcgg
     4441 gctccacata tctgatccgc gttattcctt ttatcgcaat aaaagaaaag atcaacaaca
     4501 gcttcgtgcg atcttttcta tactatattc cgtatgcagt tttaaccgcc atgactattc
     4561 cggcagtgtt ttatgctact aactggtggg ttggtgcagc agcaggactt atcgcggcag
     4621 tgatatttgc attaaagggc aaggggctta caaccgtcgc cgtggcttcc tgtgtggcgg
     4681 tatttattgt agagctgttc cactaattat ttattttccc acttaaaaga ttccatcaat
     4741 accatatccg gaatgatgga atctttattt ttaatttact ttctcctgtc tatgccactg
     4801 tcactgtaat agcgtgcctt gcttgcatac atcctcctgt ctgcttcctt ctcaatatcg
     4861 tgtacaagct cccagtcttc ctccagactt cttacacatc cgtatgagat ggtcatggaa
     4921 ttgacgattt ttcctctcca ttcggctaca ctgctgtcga aatgctttgt catagtatca
     4981 agctcatccg gcttttgagt gactatcaca acaaactcat ctccgcctat tctgtatacc
     5041 ttgccaaact caccaaagct tgccatcatg caggctgcgg ctgcgcaaat catctcatct
     5101 cccacatcat gtccatatgt atcgtttatg tgctttaagc tgtttaaatc aagtgatata
     5161 tatatccact cttttttcaa atcaagctta ttgatatctg tctcataggc atgtctgttc
     5221 aggcaccctg taagtgcatc agtattcgat atatacagca gcttttcttt ctcatatttt
     5281 tccttcataa tcttcgaaaa catatacata atacagcacg atgctagtat aagatatatg
     5341 ccaactacaa gcagtgctac tacgctgttc gtcatataaa aggatttgtc aatggtgaca
     5401 gccactatat atttgtcacc tctcctgatt acacatctgc agcctaaatc atttacgcta
     5461 atctgcctta ctgttggctt atcgctttcc ttaatatctg ttggaaggcc taaatcgtgg
     5521 aagttttttc ctattttgtc acaatctgtg gcccccttaa ccagtcctgt atctgcatct
     5581 gccacatata tttccatgcc cttgtacaca ggcatgtcag aaaccactgt agatatttca
     5641 ttctgcttca attcatctaa gagcctttgc ggcttcaaac ccacctgtac catcattgta
     5701 ccagcctcat tccaggtaat ggcatacatc attgcctttc cctctgatgt attcggtgtg
     5761 acatcctgac acatcgtaag gctcttatcg gtgagcattg gtttaaaata tgacatctga
     5821 gtccccgaat caaagctgta gccaaagtat tttggcacgc tgccgctggt tatacaacct
     5881 gtttcatcaa acagatgtat ctcatctacc gacatcagct ttgctatctt ttgcaattcc
     5941 tccacatcat tttctacctg tggctttgca tctacgatat atgacactgc ctttgccctg
     6001 actatataat cgtcctttaa cgattctata agctcctcct cactctggtc atttttatcg
     6061 agcacagtga aaacacgatc cagaagcacc tttgatagct tatatgcatt tgcatcattt
     6121 acatatttca aaattcccgc ctcgagaagc agtgctacta cgattactgc aactgagacc
     6181 attgcaaatc ttcgcttatt tttcatatcc aaattatacc aaatatatgc tgattacgct
     6241 atatttttct ataaaattac ggaccgcatg tcaccgcctg ccgtggcagc gcacatactc
     6301 cccgtaagct acgctgcgcg gctaagattc tgtacgaaat tatgcttaca gtctttctat
     6361 gcttgacgaa accggctttt atgcgccgtc catggcgcat aacgccttgc ccagccgtcc
     6421 atggctgggc atttctgtgt ttcacggcat aatttctaca gtatctaagc cgcttcgcta
     6481 ttcgcttacg gggagtatgt gcgctaccac ggcagacggc gacctgcggt acttactctt
     6541 tgctaaaact caggtacttt attgtggctt cggtatctga cagtcaacat acaggatatg
     6601 gtttatcagc cagtctgtga gatacttgag gattcccatg atctgctcat cctgatctat
     6661 ctcctcatca ctatgatgct caatgaactc atccagcttt tgtacaaatt ccatatgctg
     6721 aaccttctgt gtaaacatct tcttgtaatg tattgattcc atgtactgct cctcgtcagc
     6781 aaagtgcttt actgtgtagt cacggagctt ctccaagatg atatccatct tatcatattt
     6841 atccggtgta aactcatcat gcagcagttc atatgcctca tctgcatatt taaataactg
     6901 cttgtgctct tcatcaatca tatctattcc gatataatat tctggtttca tttcatacat
     6961 aatttcatcc ttctttctta tgtagtaata tataaacttt atgttttatc attagcaaca
     7021 ctttaacatc ccaaccaaaa tgacttatga tgtataacgt aaacaggttg ctgagcgact
     7081 atttcaagcg ccagaaccac aaaatatggt actgccggtc acatcgcagt gtggccggtg
     7141 ataaccggac agtcattaat ttatggattt tatatgcttc cggttgtaga tcagatgcag
     7201 atacatcgca tcgtgggcac ggacggcatc gtgggctgca attgcatctg caatttcacg
     7261 atgaatttcg atggtttcat cacgcatggt gaattttgca gactcacgga tcagaggaat
     7321 tgaactgttg atgataggaa ccagctttgg aacaactata tttttgctgc ttaaggcaat
     7381 agcagtatga aatgctatat ccttttcagc gtgattttta tttgcaagaa ggagcttttc
     7441 ggtattttca catgcagtaa gtatgttttt gatatctgtc tcatctgcac ggatagctgc
     7501 catggcagct atggatggtt cgattagaaa tctgatttcc atcagatctt taatcatctt
     7561 ttgtttatct tcaacaaagg tcaggccgaa tggatcatca atcatcccgg gagtggcgga
     7621 aacatatgaa ccggagcctt ggcgtatggt gatcacatta cgtgatgcga gtgctcgcat
     7681 agcttcacgc agagagcttc ttccaacatt cagccgttca cacattacgg tttcgttcgg
     7741 aagcttgtca cccggctgca gattttcctc cagtatgtat ttgataattc tctctgcaat
     7801 cctttgggaa agagtttttt ctttggaatt ttgcatgtga tttgcctcct gttgcccaat
     7861 accttgatgt ttagtttttc tgttcgttta aatattcttt caaatatttc attggcattc
     7921 tttttttctg taactcccgg aaaatattc
//
LOCUS       DPUF01000089            2573 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_18794, whole
            genome shotgun sequence.
ACCESSION   DPUF01000089 DPUF01000000
VERSION     DPUF01000089.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_18794"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="DHV81_03195"
     CDS             <1..200
                     /locus_tag="DHV81_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI93522.1"
                     /translation="KYTGNTPVQFLTALRMTNAQVLLETTSYSINEIAGIVGYNNPLY
                     FSRLFHKQKGCSPSEYRARNT"
     gene            complement(245..1192)
                     /locus_tag="DHV81_03200"
     CDS             complement(245..1192)
                     /locus_tag="DHV81_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93523.1"
                     /translation="MAYTDKLRVVYGDYTLGVHGEGFDYIFSYAQGGLESIVKNGYEW
                     LYRCPKPTFWRALTDNDRGSKFHIKSGSWLSADMFIDCKGIQVIMDGKEQNPYAPDNN
                     SYGGDVYADEIIVKYTYETITTPATTVLVSYSVDASGKIRVDVHYNGVQGLPEFPVFG
                     MRFIMPTLADKYLYKGLSGETYPDRKAGAQEGVYEIGDLSLTQYLVPQECGMRMDTEW
                     LEITRHTALDNSRTDSSSQTLRIEKNDEKFAFSCLPYTASEIENALHHEELPPARRTV
                     LCIYGAVRGVGGIDSWGTDVEEDYRISAETDKDYSFTIC"
     gene            complement(1176..2573)
                     /locus_tag="DHV81_03205"
                     /pseudo
     CDS             complement(1176..2573)
                     /locus_tag="DHV81_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341795.1"
                     /note="too many ambiguous residues; incomplete; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
     assembly_gap    1607..1637
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          741 a          605 c          391 g          805 t
ORIGIN      
        1 ggaaatatac cggtaataca ccagtacaat ttctcacagc actaaggatg acaaacgcac
       61 aagtgctgct tgaaacgaca tcctactcta taaacgagat tgcaggcatt gtcggataca
      121 ataatccact atacttcagt agattgttcc ataaacaaaa aggatgttct ccttctgaat
      181 acagagccag aaatacataa taaaaagaca aaagcaatat tatacttatc tttttaatga
      241 atgactaaca gattgtaaaa gaataatcct tatccgtctc cgcactgata cgataatcct
      301 cttccacatc tgttccccag ctgtcgatac ctccaacacc tcttactgcg ccatatatgc
      361 ataaaacggt tcttcttgcc ggtggcagtt cttcatggtg aagagcgttt tcaatctcag
      421 atgcggtata aggcagacaa gaaaatgcaa atttttcatc attcttttca atgcgcagtg
      481 tctgtgacga gctatctgtc ctgctgttat caagagccgt atgtcttgta atttctagcc
      541 actctgtatc cattctcata ccacattcct gcggaacaag atattgtgta agactcagat
      601 cacctatctc atagacgcct tcttgtgcac ctgcttttct gtcaggatat gtttccccgg
      661 agagtccctt atataaatat ttgtcagcca atgttggcat aataaatctc attccaaata
      721 caggaaattc agggagcccc tgtactccat tataatgtac atccacgcga atctttccgg
      781 acgcatcaac gctatagctc acaagaacag ttgtcgccgg agttgttatg gtctcgtatg
      841 tatattttac aataatttca tcagcataaa catcaccgcc atagctgtta ttatcaggtg
      901 catatgggtt ttgctccttg ccatccatga tcacctgtat tcccttacag tcaataaaca
      961 tatctgccga aagccagctt ccgcttttaa tatggaactt gcttcccctg tcattatctg
     1021 tcaacgctct ccagaaagta ggctttgggc agcgatacag ccactcatat ccatttttaa
     1081 cgatagattc cagtccaccc tgtgcataag agaaaatata atcaaagcct tcaccatgta
     1141 cacctaatgt atagtcacca tagaccactc ttaatttatc tgtataagcc atagtaatac
     1201 ctcacttcct gcattgccgg tttttctttc ctgtcagcaa atacaattcc atttgctgag
     1261 aattcatagt ctgccggttt atcatcaaaa tctccgccat agcgtaaaac atcttttcct
     1321 gtaacggaat cttttaccat aattgcctgg tcaataaaat cccatataaa tccgccatga
     1381 tacatatcgt atttgtcaat cagcttcata taagagccaa gtccacccat tgaatttccc
     1441 atatcatgca tgaattcgca taaaatatac ggttttttcg gactgttatt caagtattcc
     1501 tctacatctt ccggttttgc atacattctg gtctcaaaat cagaaatagt atcctcatat
     1561 gccctattat aataagagct ttcataatga actaatcttc catcctnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnctt ttcggcaaaa taaacgttca ttgcctcaat gtcgtctcct
     1681 gcataggatt catttcctaa tgaccagaaa aggatggatg tatggttttt aaaagtttcg
     1741 aaattgtttt ttgcacgctc aagcactgca tctctccact gtggaataga tccaggtaca
     1801 ttgcatgacg gttcgattgc tccaagtttt tggaatgagc catggctctc cagatttgtt
     1861 tctgccatca cataaatacc tgcatcgtcg cacatatagt accatggtat ctggtcagga
     1921 tagtgacagg tacgcacaga attaatattg tttcttaaca tacatgatat atctgccttc
     1981 atgtcttcta taccaataca tcttccggtt cttgcattcc actcatgacg gtttactccc
     2041 gtgataataa gacgttttcc attcaaatag actactttat ctataatctc tattcttctg
     2101 aaaccgatat cataaggaat tacctcggcc agtgaaccat cctctgcttt aagctcaata
     2161 taagcatggt ataaatatgg attatgatta tcccacagcc tgacactttc caagtcaaca
     2221 caaatcgtgc cttccaaatg gtcgttttct ttatttaact gtaatgactt ctcaactaat
     2281 atattttctt ctctgtcttt taaaataaag ccagcagtta cattctgact gtcagtagca
     2341 gataccttca tgctgacaga tacgcttccg ctttcattat cttgatttaa cacaggtttg
     2401 aaccatacat cctccagatg aacatccgga attgccttca aagtaacatt tctgaaaatt
     2461 ccaaaaaatc tgaaaaagtc ctgatcctct aaaaatgccg ccgtgctcat cttatgtacc
     2521 tgcactgcca gaacatttcc cttttcctta acatatggtg tcagatcaaa ctc
//
LOCUS       DPUF01000090            5594 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13174, whole
            genome shotgun sequence.
ACCESSION   DPUF01000090 DPUF01000000
VERSION     DPUF01000090.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13174"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="DHV81_03210"
     CDS             <1..502
                     /locus_tag="DHV81_03210"
                     /inference="COORDINATES: protein motif:HMM:PF09515.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93524.1"
                     /translation="IRQMLLVEIMKKHLLHHQKIHQVEPETVVQSEAQVELETVVQPE
                     TQVEPETVVQPEVQVELEAVVQPEVQVEPEAAVQPEAQVELPVVPPEAKQPSRFFFAV
                     LSGVVFFAAYASVTIGNKTIDALIYSVTYNGSYLAVEEIITIIVISIPPVKKALDYVK
                     QMANSR"
     gene            723..2030
                     /locus_tag="DHV81_03215"
     CDS             723..2030
                     /locus_tag="DHV81_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCI93525.1"
                     /translation="MEYISRIVDDVIDRKTEAFNAVSITGPKGCGKTRTARERCKTVI
                     EFQDEDKREGYLAVAETAPKLFLKNPKPILFDEWQDAPKIWGTIRKACDDNPESVGEF
                     YLTGSSSKKINTPHTGTGRITEVTMYPMTLFETGESNGSISLFKLLEDEAYDIDGLTT
                     DIKLEDLFFAVCRGGWPRCMALSKDSAKLEIAKDYYRQIYQKDISAIDGVNRNPEWAR
                     TLLWSYARNMATTAKRKNIFSDVKATQNVTDVTLSSYIDALELLYVVKDIDAWTPHLR
                     SKTAIRAAKKHIFVDPSIGLAALGVNPDYFINDLDLFGHVFENMVLRDLLVFAEKHNA
                     RVMHYTDDMGVEADAVYQLEDGRYALIEIKTGVNKIPEAEKSLLRFKDIILKYNEKAL
                     EKEKHPRDVYKEPSALIVICANATMAYTTESGVRVVPVGCLRD"
     gene            2524..2757
                     /gene="acpP"
                     /locus_tag="DHV81_03220"
     CDS             2524..2757
                     /gene="acpP"
                     /locus_tag="DHV81_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCI93526.1"
                     /translation="MLEKIKEIVADQLGIDEDDIKLESNFKEDLEADSLDLFELVMAL
                     EEEYGVEIPSEDLEKIATVNDIIEYLKNKGVEA"
     gene            2763..>3073
                     /locus_tag="DHV81_03225"
     CDS             2763..>3073
                     /locus_tag="DHV81_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HCI93527.1"
                     /translation="METRITKLLGIKYPVIQGGMAWVATHELASAVSNAGGLGIIGAG
                     GAPASWVKEQIVAAKKETDKPFGVNLMLMNPEADEIAKIIAEEKVKVVTTGAGNPAKY
                     M"
     assembly_gap    3074..3177
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3178..3687
                     /locus_tag="DHV81_03230"
     CDS             <3178..3687
                     /locus_tag="DHV81_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HCI93528.1"
                     /translation="VIAEGMESGGHIGAQTTMTLVPQVVDAVSIPVIAAGGIADNRGF
                     NAAFALGAEAVQMGTRFVTAKESIVHENYKQKVIKAKDIDSEVTGMSTGHPIRVLRNK
                     MTRQYLKLEKEGAPFEELEKMTLGALRKAVVDGDTVYGSVMAGQSAGLVKKEETCKEI
                     IEDVVGLNK"
     gene            3781..4707
                     /gene="fabD"
                     /locus_tag="DHV81_03235"
     CDS             3781..4707
                     /gene="fabD"
                     /locus_tag="DHV81_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[acyl-carrier-protein] S-malonyltransferase"
                     /protein_id="HCI93529.1"
                     /translation="MGKRVFMFPGQGSQYIGMGKEFYDTMPNAKAVIDLASEQTGLDI
                     PALCFEENDKLNITEYTQICMLAVEAAIYQAIIDKGITPDVTAGLSLGEYGALIASGA
                     MTMADAFAVVRKRGIYMQEAVPTGGAMTAVLGLDPQVIEDICKKTADETNSVVSVANY
                     NCPGQIVITGEKAAVDAAAAACSEAGAKRCVPLKVSGPFHSAMLKGAGEKLADALESV
                     EIHDIKVPYITNVTADYVKSPADVKDYLTKQVSSSVRWQQTIERLIADGADEFVEIGP
                     GRSLSGFMRKINRDVKVVNIDKLEDFEKYVNR"
     gene            4694..5434
                     /gene="fabG"
                     /locus_tag="DHV81_03240"
     CDS             4694..5434
                     /gene="fabG"
                     /locus_tag="DHV81_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCI93530.1"
                     /translation="MLTGKNALVTGASRGIGRQIALTLAAAHAFVIVNYNGSKEKADA
                     VVAEIKAAGGDAVSYQCNVSDFDECQNMITALIKEYKHIDILVNNAGITRDGLIMKMS
                     EADYDAVLDTNLKGAFNTIRHMSRYFLKQKSGRIINISSVSGILGNAGQANYSASKAG
                     VIGLTKAVARELASRGITANAVAPGFVETEMTDVLADAAKENLLSQIPLGRPGNTKDI
                     ANAVLFLASDAAGYITGQVLSVDGGMAI"
     gene            5431..>5594
                     /locus_tag="DHV81_03245"
     CDS             5431..>5594
                     /locus_tag="DHV81_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HCI93531.1"
                     /translation="MSRRVVVTGLGAVTPIGNNVDDFWTSVKAGKIGFDHITKFDTTD
                     YKCHIAAELK"
BASE COUNT         1822 a         1020 c         1411 g         1237 t
ORIGIN      
        1 aataagacag atgctgctgg tggaaatcat gaaaaaacac ctactacacc atcagaaaat
       61 ccatcaggtg gagccggaga cagtggttca gtcggaggca caggtggagc tggagacagt
      121 ggttcagccg gagacacagg tggagccgga gacagtggtt caaccggagg tgcaggtgga
      181 gctggaggca gtggttcaac cggaggtgca ggtggagccg gaggcagcgg ttcaaccgga
      241 ggcacaggtg gagctaccgg tggtaccacc ggaggcgaag cagcccagta gatttttctt
      301 tgcagtgctg tcgggagttg tgttctttgc agcatacgca tctgtcacga taggaaacaa
      361 gacgatagat gcgctcatat attcagtgac ctacaacggc tcataccttg cagtagagga
      421 aattatcaca atcatagtga tatcaatccc acctgttaag aaggcgctcg actatgtgaa
      481 gcagatggcg aacagccggt agataatcta tatttaaata attaaaatat gtaaaaaaga
      541 gcagaatagg tagaaatact tgtcctgctc tttttagtat tcgaaaaaag taaaataaaa
      601 cactcgaaaa atataaaata aaacattaga aaaatataaa acaaaacact cgaaaaacat
      661 aaaataacct tgtataatct gtgaattaaa tggtataata accatgaagg gagcagatat
      721 ttatggaata tataagcaga attgttgatg atgtgataga tagaaaaaca gaggcattta
      781 atgcagtgag catcacagga ccaaaaggat gtggaaagac aagaacagca agagaacgat
      841 gtaaaactgt tattgaattt caggatgaag acaagagaga ggggtatctt gcagtagcag
      901 aaactgcacc taagctcttt ttaaagaatc ctaagccaat actgtttgac gagtggcagg
      961 atgccccaaa gatatgggga acaataagaa aagcgtgcga tgacaatccg gagagtgtag
     1021 gagagtttta tctgacaggc tcatcaagta agaagattaa tacaccccat acaggtacag
     1081 gacgaataac agaggttacg atgtacccta tgactttgtt tgagacagga gaatcaaacg
     1141 gaagcatatc gttgtttaaa ttattagaag atgaagcata tgatattgat ggacttacca
     1201 cagatataaa gcttgaggat ttgttttttg cggtctgtag aggtggatgg ccgagatgca
     1261 tggcactttc aaaggacagt gcaaagcttg aaatagctaa ggattattat aggcagattt
     1321 atcagaagga catatcagcg attgatggag taaacagaaa tccggaatgg gcaaggacac
     1381 tgctttggtc gtatgccaga aatatggcta ctacagcaaa acgcaagaat atattttcag
     1441 atgtaaaagc cacacagaat gttacagatg taacgctttc ttcatatata gatgctctgg
     1501 agctattgta tgttgtaaag gatatagatg cctggacacc gcatttgcgt tcgaaaacag
     1561 ctatcagagc tgccaaaaag catatatttg ttgatccctc tataggattg gctgcgcttg
     1621 gtgttaatcc ggactatttt atcaacgatt tggatctatt tgggcatgta tttgaaaata
     1681 tggtgcttag agatttactt gtatttgctg aaaagcacaa tgccagagtg atgcattata
     1741 cagatgatat gggcgtagaa gcagatgcag tctatcagtt ggaggacggc cgttatgcgc
     1801 ttattgaaat aaaaaccgga gtgaacaaga ttccggaagc ggaaaaatcc ttgctgcgat
     1861 tcaaggatat tatcttaaaa tataatgaga aagcattgga aaaagaaaaa catccacgtg
     1921 atgtatataa agaaccatcg gcactgattg tgatttgcgc aaatgcaacc atggcatata
     1981 ccacggagtc aggagttcgg gttgtgccgg ttgggtgttt gagagattaa tatagagcag
     2041 actgtaaaat tgagctgacc actcataatt acagtctgtt ttttattgta tattattgtc
     2101 acagtgtaag ggagcaggcg ggcagaaaat tgcccatcca atgagtttgt acgaagctgc
     2161 ttagaggctg ttggaattgt aataggtaca cagtggcgca cgtgcatgga agcacgggcg
     2221 aggcgttacc gcgccaagga cggcgcgtaa aagccggctg cgtcaggcat agaaaacctg
     2281 acttacaatt cttacagcct cttggctgct ggtaggagac gttttggatg ggtaattttc
     2341 tgcccgcctg ctcccgtacc ataaaaaatg aaaaataaaa atactttgac ttgacaacta
     2401 ttttaaatgt gatatacttt cactgtcaaa tgatttgatg atgaaaatat ttgacatgca
     2461 aactagtgca aatataccag cacacaaaac cgaaaataaa cacagaaaaa ggagaacaga
     2521 aatatgttgg agaaaatcaa ggaaatcgta gcagatcagt taggaattga cgaggatgat
     2581 atcaaattag agtcaaactt caaggaggat cttgaggcag attcattaga tcttttcgag
     2641 cttgtaatgg cactcgagga ggagtacgga gtagaaatcc cttcagagga tttagagaaa
     2701 atcgcaacag taaacgacat cattgagtat cttaagaaca aaggtgtaga ggcttaaaag
     2761 acatggagac aagaataaca aagcttcttg gaatcaaata tccggtaatt cagggtggca
     2821 tggcatgggt agcaacccat gagcttgcca gcgctgtgtc caatgcaggc ggacttggca
     2881 tcatcggagc aggcggagcc ccggcttcat gggtaaagga gcagatagtc gctgcaaaga
     2941 aggagacaga taagccgttc ggagtcaacc tcatgcttat gaatccggag gccgatgaga
     3001 tagcaaaaat catcgccgag gaaaaggtaa aggtagtcac caccggcgca ggaaaccctg
     3061 caaagtacat ggannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta
     3181 atcgccgagg gaatggagtc aggcggacat atcggagcac agactaccat gacacttgtg
     3241 ccacaggttg ttgatgcagt gagcatccct gttatcgcag caggcggaat tgcagacaac
     3301 agaggcttca atgcagcatt tgcactcggt gcggaggcag tccagatggg tacacgcttt
     3361 gtcaccgcaa aagaatcgat agtacatgaa aactataagc agaaggtcat taaggccaag
     3421 gatatagatt ccgaggttac aggaatgagc acaggccatc cgatcagagt gctcagaaac
     3481 aagatgacca gacaatatct caagctggaa aaggaaggag ctccatttga agagctcgaa
     3541 aaaatgacac ttggcgcact cagaaaagcc gttgtagacg gagataccgt atatggcagc
     3601 gtcatggcag gtcagagcgc aggcctcgta aagaaagagg aaacctgcaa agaaatcatt
     3661 gaggatgtag tgggactgaa caaatagaca gtattcactt ttacatgcaa atttaaggat
     3721 accgggccgc ctgcaaagag tggcttacgg tatcctgtca gaaataagga gaaaccgaaa
     3781 atgggtaaga gagtatttat gtttcccgga cagggctcac agtatatagg aatgggtaag
     3841 gagttttacg atacaatgcc aaatgcaaag gcagtaatcg accttgccag tgaacagaca
     3901 ggcttggata ttccggcatt atgctttgag gaaaatgaca agctgaatat tacagagtac
     3961 acacagatat gtatgcttgc agttgaggca gctatatatc aggcaatcat tgacaaaggt
     4021 atcacacctg atgtcacagc aggcttaagc cttggggaat atggtgctct catcgcatca
     4081 ggagccatga ctatggcaga tgcattcgca gttgtcagaa aaagaggaat ctacatgcag
     4141 gaggcagttc ctacaggtgg agccatgaca gcggttttag gacttgatcc gcaggtcatt
     4201 gaggatatct gcaaaaagac agcagatgag acaaattcag tggtatcggt agccaactat
     4261 aactgcccgg gacagattgt tatcacagga gaaaaggcag cagtagatgc cgccgcagca
     4321 gcatgcagcg aggcaggcgc caagagatgc gtaccgctca aggtcagcgg acctttccat
     4381 tcagctatgc ttaagggcgc aggcgaaaag ctcgcagatg cacttgagag tgtagaaatc
     4441 cacgatatca aggtgccata tatcacaaat gtcacagcag actatgtgaa gtcaccggca
     4501 gatgtcaagg attatctgac aaagcaggtt tcatcgtcag tacgctggca gcagaccatc
     4561 gagcgcctta tcgcagacgg tgcagatgag ttcgtggaaa tcggaccggg acgatcactc
     4621 agcggattta tgagaaagat aaatagagac gtaaaagtcg taaacataga caaactggag
     4681 gattttgaaa agtatgttaa caggtaaaaa tgccctggtt acaggcgcaa gccgcggaat
     4741 tggaagacag atagctctta cacttgccgc agcacacgcc tttgtaatag taaattataa
     4801 tggttcaaag gaaaaggcag atgccgttgt tgcagagata aaggcagcag gcggtgatgc
     4861 agtttcttat cagtgcaatg tatctgattt tgacgagtgc cagaatatga taacagcgct
     4921 catcaaggag tacaagcaca tagatatcct cgtaaacaac gcaggaatca caagagacgg
     4981 tctcatcatg aagatgagcg aggcagacta tgacgcagtg ctcgacacaa accttaaggg
     5041 tgcgttcaac acaatccgtc atatgtcacg ctatttcctg aagcagaaat caggaagaat
     5101 tatcaatatt tcatcagttt ccggaatact cggaaatgca ggacaggcca actacagcgc
     5161 cagcaaggca ggagttatag gactcacaaa ggctgtagcc agagagcttg ccagccgggg
     5221 aatcacagca aacgcagtgg caccgggctt tgtagagaca gagatgacag atgtgcttgc
     5281 cgatgcggca aaggagaatc ttttatcaca gattccgctt ggcagaccgg gaaacacaaa
     5341 ggatatcgca aacgcagtat tatttttagc atcagatgca gcaggctaca tcacaggcca
     5401 ggtgctgtct gttgacggag gaatggcaat atgagtagaa gagtagttgt aacaggactt
     5461 ggagcagtaa caccaatcgg aaataatgtg gacgatttct ggacatcggt aaaggccgga
     5521 aaaatcggat ttgaccacat cacaaaattt gacacaacag attataaatg tcacattgca
     5581 gcagagctta agga
//
LOCUS       DPUF01000091            6481 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8472, whole
            genome shotgun sequence.
ACCESSION   DPUF01000091 DPUF01000000
VERSION     DPUF01000091.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8472"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="DHV81_03250"
     CDS             <1..693
                     /locus_tag="DHV81_03250"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516589.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCI93532.1"
                     /translation="ALYKSMEFVGDGLKYLSMDDRFTIANMAIEAGGKNGIFPVDDLT
                     REYMKEHSKRPFTEYEADSDAEYDEEYTIDLSILKSTVSFPHLPDNTRTIDEVGDVKI
                     DQVVIGSCTNGRMDDLRIAAKILEGKKVADGIRVIVIPATQKIYLQAMEEGLLKTFIE
                     AGAIVSTPTCGPCLGGYMGVLAEGERCVSTTNRNFVGRMGHVDSEVYLASPAVAAASA
                     ITGKISGPKDVM"
     assembly_gap    706..891
                     /estimated_length=186
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <892..1191
                     /gene="leuD"
                     /locus_tag="DHV81_03255"
     CDS             <892..1191
                     /gene="leuD"
                     /locus_tag="DHV81_03255"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516588.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCI93533.1"
                     /translation="CGSSREHAPIAIKAAGVSCVIAETFARIFYRNAINIGLPIIECP
                     EAAKAIKAGDEVTVDFDSGIITDETTGEKFKGQAFPPFMQKIIDCEGLVNYINQK"
     gene            complement(1281..2081)
                     /locus_tag="DHV81_03260"
     CDS             complement(1281..2081)
                     /locus_tag="DHV81_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93534.1"
                     /translation="MLQGVYTAVRKDGTIYYRSNITYKSKHISLGSFDSELSASRAYA
                     TASTILLKETNSIEDSYFHTHALTFDKIVTLINFRDNGMYIKTPIYLRKNYFSYYLDI
                     SHELKFDIDDLFYYSEHRILKRQGHLYVNDYGMQVTLLSRYGIQPHAVCGRDYVFVNN
                     DSTDMRYENIKLLNPYHGVEIIKTAGLDKYKARIHINGNFVIGTYNTIEKAAIAYNKA
                     VDMAHAHGIEKNYPENYIESISGKEYADIYTSVTVSNKYLQYLQQFDL"
     gene            2255..3753
                     /locus_tag="DHV81_03265"
                     /pseudo
     CDS             2255..3753
                     /locus_tag="DHV81_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569505.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
     assembly_gap    2561..2637
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3916..4794)
                     /locus_tag="DHV81_03270"
     CDS             complement(3916..4794)
                     /locus_tag="DHV81_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HCI93535.1"
                     /translation="MYTQFFGNFLLSKGYITNEQLFDALKEKAQKHAKLGTLAIHSGL
                     MTAAEVDSVIVEQTHQDKKFGELTIEMGYLTDEQVKDLLNIQSPDFLLLGQILLDKGI
                     IDNTTLEKSIHDYRSENAISDLDMVLEDKDSINHLIGHFFANTGIDPSAIDIMYLELL
                     FNSFIRFVGDDYTPLSAEICDSFSADCMVRQDIEGSYAISTYIGMSQTTAINFASRYV
                     NESFSVYDEYVQASLDDFLNLNNGLFLVNCSNDQALELTLTAPEHFDGQTRSFNKACK
                     LKLLYPFGAIHFIIEF"
     gene            complement(4796..5173)
                     /locus_tag="DHV81_03275"
     CDS             complement(4796..5173)
                     /locus_tag="DHV81_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCI93536.1"
                     /translation="MQLDDLKILICDDSILARKQMKDIINSIGSPTIIEAANGQLAID
                     MYKEHKPNLTFIDIVMPVKDGNAAISEIMEYDSSATIFVASSVGTQSELKKALAAGAC
                     DFIQKPIDKDQVISLIKKHIGGN"
     gene            5378..5719
                     /locus_tag="DHV81_03280"
     CDS             5378..5719
                     /locus_tag="DHV81_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93537.1"
                     /translation="MNYFIIIDAVTLILGIYIIFVSLRMKKSGKIESTFVAEDEMKKI
                     KDTAGYIAYIYPKSLVFGIVIFVISIVAIVSDCLKKIPYWSYVEMIIFVAVIIWFSNM
                     LRNAREKFVKF"
     gene            5772..5955
                     /gene="ssrS"
                     /locus_tag="DHV81_03285"
     ncRNA           5772..5955
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="DHV81_03285"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6167..>6481
                     /locus_tag="DHV81_03290"
     CDS             6167..>6481
                     /locus_tag="DHV81_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein SpoIID"
                     /protein_id="HCI93538.1"
                     /translation="MVWIMLAITVVCVVIVFAIVMAQKEKGALVKQARAVTKDMVYEN
                     AYIVSNDDGRLIFICDGDLYRAKGTMEENFTGVCDIEISGSKVKKIQIKPDDISGVML
                     SYG"
BASE COUNT         1909 a         1089 c         1393 g         1827 t
ORIGIN      
        1 gcactttata agtcaatgga gtttgtcgga gatggactta aatatctttc tatggacgac
       61 agatttacta tcgccaatat ggcaatcgag gccggcggca agaatggtat cttccctgta
      121 gatgatctca caagagagta catgaaggag cattcaaagc gtccgttcac agagtatgag
      181 gctgatagtg atgcagagta tgatgaggag tatacaatcg accttagcat acttaagtct
      241 acagtttcat tcccacatct gcctgacaat acacgtacca tcgatgaggt gggtgatgta
      301 aagattgacc aggtagtaat cggttcatgt accaacggcc gtatggatga tctgcgtatt
      361 gcagcaaaga ttcttgaggg taaaaaggtt gcggatggta tcagggttat cgtgattcct
      421 gccacacaaa agatttatct tcaggctatg gaagagggcc ttcttaagac atttatcgag
      481 gcaggtgcca ttgtgagcac acctacctgc ggaccatgtc ttggcggata catgggagtg
      541 ctcgctgagg gcgagagatg cgtatctacc acaaaccgta actttgtcgg acgtatgggt
      601 catgtggatt ctgaggttta tctggcaagc cctgctgttg cagctgcaag tgccattaca
      661 ggtaagattt caggaccaaa ggatgttatg taaagattgg aggaannnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgtggttca
      901 tcaagagagc atgcaccgat tgccatcaag gctgccggcg taagctgtgt tatcgcagag
      961 acatttgcaa gaattttcta cagaaatgca atcaatatag gtcttcctat tattgagtgc
     1021 cctgaggcag caaaggcaat aaaagccggt gatgaggtga cagtagactt tgattcaggt
     1081 atcatcacag atgagacaac aggagagaag tttaagggac aggccttccc accgttcatg
     1141 cagaagatta ttgactgcga gggacttgta aactatataa atcagaagta attataaaaa
     1201 ttcccgaggt tatataaccg cgggagtttt tagtacaata ttaaaaataa tggtaatatg
     1261 caaactgtgg gatatgcgtt ctataaatca aattgctgca gatactgcaa atatttattg
     1321 gaaactgtca cagaagtgta aatatccgcg tattccttgc ctgatatact ttcgatatag
     1381 ttttcgggat agtttttttc tatgccatgt gcatgagcca tatcgacagc tttattgtat
     1441 gcaatagcgg ccttttcgat tgtgttgtag gttcctatga caaaattacc gtttatgtgt
     1501 atgcgagctt tatatttgtc gagccctgca gttttgataa tctctacacc atggtatgga
     1561 tttaacagct tgatgttttc gtagcgcatg tcggttgaat cgttgtttac aaatacatag
     1621 tcccgtccgc aaacagcatg aggctgtatg ccatagcggc taagcagggt gacctgcatg
     1681 ccgtagtcgt tgacgtacag atgtccctgg cgttttaata tgcgatgctc agagtagtaa
     1741 aacaaatcgt ctatatcgaa tttcagctca tgtgatatat ccagatagta gctgaaatag
     1801 tttttgcgca gatatatggg cgtctttatg tacattccgt tatcacggaa attaataaga
     1861 gttacaattt tatcaaatgt aagtgcatgg gtgtgaaagt aagagtcctc tatgctgttt
     1921 gtttctttta ataaaattgt cgatgcagtg gcataggcgc ggcttgcaga aagctcactg
     1981 tcaaagcttc cgagactgat gtgtttgctt ttgtaggtga tgttggacct gtagtaaatg
     2041 gtgccgtctt ttctgacagc ggtgtaaact ccctggagca tagatataat ttgtgttcct
     2101 ttcacatata tttttaatca tatatatatt attgtagcat ttttgttatt gatttgatat
     2161 atgaaattta ttatactata aaggataata attgtctgca tatagatgca gcaatatgga
     2221 tttagataag ataaaaagag aaatgaggta aaaaatggat ttattatatt caagtacaag
     2281 aaacagtgaa aagaaaataa cagcttcaca ggctatcctt aagggcattg ccgatgacgg
     2341 aggattattt gtacctgaga gcattccggc acttgatgtt tctgttgagg agctttctaa
     2401 gatgtcatac cagcaggtag catatgaggt tatgaagctt ttccttacgg atttcacaga
     2461 ggaagagctt aaaaattgta tcaacagggc atacgacagc aagtttgata ctactgagat
     2521 tgcaccgctt aagtttgctg acggggctta ctaccttgag nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca
     2641 aagaaaaaca atgttaaaaa cgagattgtc atcctgacag ccacatcagg agacacagga
     2701 aaggcagcta tggcaggttt tgctgatgtt gaggggacac agattatcgt tttctatcca
     2761 aagaatggtg tcagcccgat tcaggagaag cagatgctta cacaaaaggg tgccaatact
     2821 catgtagtgg gtattcacgg caactttgac caggcacagt cagcagtaaa ggctatgttc
     2881 aatgataagg ctcttgcaga gacgatggat gccgcaggct atcagttctc atccgccaac
     2941 tcaatcaata taggtcgcct tgtaccacag attgtgtact atgtatatgc ttactcaaag
     3001 ctttttgcac agggagctgt cgcaaaggat gagaagataa atatcgtagt tcctacggga
     3061 aactttggaa atatacttgc agcgttttat gcaaagaaca tgggacttcc cgttgcaaag
     3121 cttatctgcg cttcaaacga gaacaaggtg ctttacgatt tcttcacaac aggagagtat
     3181 gataaaaaca gagagtttat cctcacaaat tcaccatcca tggatattct catctcttca
     3241 aaccttgaga gactcattta ccgtatcgca ggaaatgatg caaataagaa tgctcagctt
     3301 atggcttcgc ttgctaagga tggcaaatac actgtaacac ctgagatgaa ggaaaagctt
     3361 tctgacttct atggaaatta cacaagtgag aaggaaaccg ctgaggttat caaaaagctt
     3421 tacgaggata caggatacat tattgatact catacagcag ttgcagcagg tgtatatagc
     3481 aagtataagg aggctacagg tgatacagcc acaaagactg ttattgcatc tacagcaagt
     3541 ccattcaaat tcacaagaag tgtgatggat gcaattgatc ctaagtacga ttcaatgaca
     3601 gactttgagc ttgtagatga gctatcaaag cttggcaacg ttaaggtacc aaatgccatc
     3661 gaggaaatca gggatgcaaa gattctccac aacacagtct gtgatgtgga tcagatggaa
     3721 aacactgtta agaaaatgct cggtgtgcag taataatata atgttacctt ttgcccattt
     3781 aatcatataa tttgtttgat atataattat atatagcgca acataaaaga acatctgctg
     3841 attgcagatg ttctttttgt gctctaatta tagagtaaat aattatcaga ttactgaaac
     3901 tgccttgcag agatattaga attcaataat aaagtgaatg gctccgaaag ggtataacag
     3961 cttgagcttg caggctttat tgaagctgcg tgtctgtcca tcaaaatgct caggtgcagt
     4021 gagagtaagc tcaagtgcct gatcatttga acagttgacc agaaacagtc cattgttcag
     4081 gttcaggaaa tcatcgagag atgcctgtac atactcgtcg tatactgaaa atgattcatt
     4141 tacatagcgt gatgcaaaat tgatggctgt tgtctgactc atgcctatgt atgtagatat
     4201 ggcatagctg ccctcgatgt cctgacgtac catgcagtcc gcactaaagc tgtcacatat
     4261 ctcggcagag agcggagtgt agtcatcacc gacaaatcgg ataaagctgt taaacaaaag
     4321 ctcaagatac ataatgtcaa tggcagaagg atctatgcct gtattggcaa aaaaatgacc
     4381 tatcagatga tttatactgt ccttatcctc gagtaccatg tcaagatcgg aaatagcatt
     4441 ctcactgcgg taatcatgta tactcttttc gagagtggta ttgtcaatta tgcctttgtc
     4501 taaaagaatc tgtcctaaaa gaagaaagtc agggctttgg atgtttaaga gatcctttac
     4561 ctgctcgtct gtcagatagc ccatttctat tgtaagctca ccaaacttct tgtcctgatg
     4621 ggtctgctcg actattactg aatcaacctc ggcagctgtc ataagccctg agtggatggc
     4681 aagagtaccg agctttgcgt gcttttgtgc tttttctttg agtgcgtcaa aaagctgctc
     4741 attagtgata tacccctttg ataggagaaa attgccaaaa aattgagtat acatattaat
     4801 ttcctcctat gtgcttctta atcagactta tgacctgatc cttgtcgatt ggtttctgaa
     4861 tgaagtcaca tgctccggca gcaagagcct ttttaagctc ggactgtgtg ccgacagatg
     4921 atgcaacgaa gatggtagca ctgctgtcat actccataat ttctgagata gcggcatttc
     4981 cgtcctttac cggcattaca atgtctataa atgtgagatt tggcttatgc tccttgtaca
     5041 tatcaatggc gagctgtccg tttgccgcct caatgatagt tggtgaacca atggaattga
     5101 ttatgtcctt catctgtttt cttgcgagaa tcgaatcatc gcatataagt atttttaaat
     5161 cgtcaagctg cattttaaaa actccttccc cttgaaaata taaatatatt ctactacatt
     5221 aataataaaa attcaagaaa tagttgggac ttatttacca tttatttttt ccttacatga
     5281 ggttatacta attttggtga ttttacatat tttttgtata tataagatcc gtgataacta
     5341 tttaggaata ataaataaaa atacaggaga aagaagcatg aactatttta ttataataga
     5401 tgcggtcacg ctgatactag gaatttacat aatatttgtg tcactacgaa tgaagaaaag
     5461 cggcaaaatt gaaagtacct ttgtggctga ggatgagatg aaaaaaataa aggatacggc
     5521 cggatatata gcttatattt atccaaagtc acttgtattc ggcattgtga tttttgttat
     5581 cagtatagtt gcgatagttt cggattgcct caaaaagata ccgtactggt catatgtgga
     5641 gatgattata ttcgtcgcag taattatctg gttttcaaat atgttacgaa atgccagaga
     5701 aaaatttgtt aaattctaac atgaattttg cattgcaaca tgagacataa taaagtataa
     5761 tgaatttaca aagatacctg agatgttcga caaccctgtg tatttagaac ctacatttac
     5821 tttaaacggg attcctatat gagctgttgg atgtcaggcc gtcattatgc agcggaaggc
     5881 agaaggcaga ctgcggttac ccacctagca agagcgctag gagtctaaat tacaggaccg
     5941 gcattttggg ttcttacaaa agaataataa tgtacaaaag aactccggat gcacaaaaga
     6001 tttatgagat acaaaagatt tttaaagcac aaaggattct taagtacaga tgataaaggg
     6061 agctgttttt gcagctccca ttttttgagc agtaaccact atttgggtgt taaggagaac
     6121 aagaaatttg gcaaatagaa gacgaagacg gcaaaagccg aatcagatgg tctggataat
     6181 gcttgcgata accgtggtat gcgtggttat tgtttttgca attgttatgg cgcaaaagga
     6241 aaagggggca cttgtaaaac aggcgagggc cgtgacgaag gatatggtgt atgagaatgc
     6301 atatattgtg tcaaatgacg atggaaggct gattttcata tgtgatggag atctatacag
     6361 ggcaaagggt accatggaag agaactttac aggtgtctgt gatattgaaa tcagtggctc
     6421 taaggtcaaa aagatacaga taaagccgga tgatatatca ggtgtgatgc tgtcgtacgg
     6481 a
//
LOCUS       DPUF01000092            5113 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_14776, whole
            genome shotgun sequence.
ACCESSION   DPUF01000092 DPUF01000000
VERSION     DPUF01000092.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_14776"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="DHV81_03295"
     CDS             <1..708
                     /locus_tag="DHV81_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCI93539.1"
                     /translation="DIIYEDNDIIVCYKPAGIATQTRRIGQQDMESFIRNYRAAKNEP
                     PYVGIVHRLDQPVEGVMVFAKNQKAAASLSRQIKEHTTEKYYRAASRGQGVSGADKEE
                     ANKEDNHDLAWHTLTDYISFDKLTNTSKITSEKDKLAKKAVLQYRIISNECNKTEFDI
                     KLLTGRHHQIRLQLANIGYPLIGDTKYGKPASNNSGTGSKSGRTGGGVREQLALCSYK
                     LVFDHPATGERMTFELP"
     gene            complement(1132..1629)
                     /locus_tag="DHV81_03300"
                     /pseudo
     CDS             complement(1132..1629)
                     /locus_tag="DHV81_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858365.1"
                     /note="frameshifted; incomplete; missing start; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    1630..1644
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1724..2104
                     /locus_tag="DHV81_03305"
     CDS             1724..2104
                     /locus_tag="DHV81_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93540.1"
                     /translation="MDKKKRLLILIFVLILSGIVVIFIHKNGSMKGDSIMEENAISGD
                     DNTHWFHSNGIITSIDNSQKKICVDISQKNNFFDGTNITLNCNKSSLDITYLEAGQEI
                     TFYFFENNVLDTEVAIEKLNIVTP"
     gene            2401..2676
                     /locus_tag="DHV81_03310"
     CDS             2401..2676
                     /locus_tag="DHV81_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006095115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI93541.1"
                     /translation="MNEILNQEKSIYIQISEMIENDILRDVLLEDERVPSTNELAKLY
                     AINPSTAAKGINILVDAGILYKKRGIGMFVSDGAKEVIRTKRKARFC"
     gene            2841..4124
                     /locus_tag="DHV81_03315"
     CDS             2841..4124
                     /locus_tag="DHV81_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCI93542.1"
                     /translation="MKKKFVAIMMVAAMAASMAACGSDGGSSGTQKGGSSTSTSDVAN
                     KDKPLVWFNRQPSNSSTGELDTTALNYNKDTYYVGFDANQGAELQGEMVKEYIEKNID
                     TIDRNGDGVIGYVLAIGDIGHNDSIARTRGVRKALGTGVDKSGEIDSAPAGTNSDGKA
                     AEVQDGKITVNGKDYVVRELASQEMKNSAGATWDAATAGNAIGTWSSSFGESIDVVVS
                     NNDGMGMSMFNAWSKDNKVPTFGYDANSDAVAAIAEGYGGTISQHADVQAYLTLRVLR
                     NALDGVDIDTGIGTEDDAGNVLSDDVYVYKDDERSYYALNVAVTADNYKDFTDSTVVW
                     APVSTQLDSAKHPTKKVWLNIYNASDNFLSSTYQPLLQKYDDLLNLDVEYIGGDGQTE
                     SNITNRLGNPSQYDAFAINMVKTDNAASYTALLNQ"
     gene            4219..>4445
                     /locus_tag="DHV81_03320"
     CDS             4219..>4445
                     /locus_tag="DHV81_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCI93543.1"
                     /translation="MGDKNNENILSIRGMSKSFGRNRVLDHINLDVKKGTVMGLMGEN
                     GAGKSTMMKCLFGTYQKDEGTIYLDGKEVSFS"
     assembly_gap    4446..4628
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4629..>5113
                     /locus_tag="DHV81_03325"
     CDS             <4629..>5113
                     /locus_tag="DHV81_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCI93544.1"
                     /translation="NMSVSQRQMCEIAKAISYNSKVIVLDEPTSSLTVQEVEKLFQMM
                     RMLRDQGISLIYISHKMDEIFEICDEISVLRDGNLVMTKSTKETNMNELIAAMVGRVL
                     ENRFPPVDNKPKDVILSIQHLSTKYEPHLQDITFDVHEGEIFGLYGLVGAGRTELLET
                     I"
BASE COUNT         1666 a          846 c         1121 g         1282 t
ORIGIN      
        1 gatatcattt acgaagacaa cgacattatc gtctgctaca agccggcggg aattgctaca
       61 cagaccaggc gtatagggca gcaggacatg gagagcttca taagaaatta cagggcggct
      121 aaaaatgagc cgccctatgt cggtatagtg cacagactcg accagccggt agagggcgtg
      181 atggtatttg ccaagaatca gaaagccgcg gcatccttga gcaggcagat aaaggagcat
      241 acaaccgaga aatattatcg tgcggcaagc agaggtcagg gagtgtcagg agcagataag
      301 gaagaagcta ataaagaaga taatcatgat ctggcatggc atactctcac agattatatc
      361 tccttcgaca agcttaccaa tacatctaaa atcacaagcg aaaaagacaa gctggcaaaa
      421 aaagcagtgc ttcagtacag gataatatca aatgaatgca ataaaacaga atttgacata
      481 aagcttctca ccggaaggca ccatcagata cgtttgcagc ttgcaaatat tggctatcca
      541 ctgataggag ataccaagta tgggaagcct gcctcaaata actcaggaac gggaagcaaa
      601 tccggacgca ccggtggcgg tgtgagggag cagcttgcac tttgctccta caagcttgtt
      661 tttgaccatc ctgcgacagg ggagaggatg acatttgaat taccatagtg ctgttgggaa
      721 taagatgaat taaagtgtat ggtctttttt atatgaagaa aaatacagtt gacaatatta
      781 tttatattat ctattataag ttcataggtt agttacttga gtggttaacc atgcaaccat
      841 accgcaaata tgagcgaaat tagctgtgta gcagcgatgc taaacagagt gcagataaag
      901 tacaaatatc gtgcggtttt gcgaatgtgt gtggtggttc tggatagtta cgataataaa
      961 aagtacaaca agtaggtaat tgcggaacaa gtgcgcactc ctgattaaaa agcggtaaga
     1021 attctgcaac gtaggattct taaagatgaa aagtaggtgc ttctaaattt agacatgact
     1081 aaaagatttc ccagataaac tgcgaagaaa agattatatg catattaata atcaggcaat
     1141 caagggcttc aaactccgaa tgtatttgta ctgatgaatc ttatcagcga caattacagt
     1201 aacaagctgg gtgattccgg caagaagtaa atcagcatga agcgttttct tattctgggt
     1261 cttacgattc gcaatgttgc aactatcttt gaaatggttg attgaatttt caacatttac
     1321 acggatcttg taagtatttt cccattcttt cgagctacgt tctatgccag gataggcacg
     1381 gagaggaata aaagcattac gaaatccaat gtcttcgaag atcgtaaatc caacaagaag
     1441 tcctgtttaa aacgtgtaaa tttggaacca tctggaacag catcaaagtc gcagaaatca
     1501 cgaagttcct gagagtactc taggaacgca atcaggagcg tatctgtcgg gattgaaaaa
     1561 aatacgctgg agtagaagtg ccttgagcat cggataaagc tggtgctttc gaggtattcc
     1621 ggttgaagcn nnnnnnnnnn nnnntttgat ggttggaatg aatatgtacg atatttaaat
     1681 ttagattttg gaaactggac tgatttgcga ggtacaactt ttaatggata aaaaaaagcg
     1741 actacttatt ctaatatttg tattaatatt aagcggaatt gttgtgatat tcatacataa
     1801 aaatggttcc atgaaaggag attctattat ggaagaaaat gctatttccg gagatgataa
     1861 tactcattgg ttccattcaa acggtatcat cacatccata gataactctc aaaaaaaaat
     1921 ctgtgttgat atcagtcaaa aaaataattt ttttgatggt accaatatta cattaaattg
     1981 caacaaatca tctctcgaca ttacatattt agaggctggt caagaaatta ctttctattt
     2041 ctttgagaat aatgtacttg acactgaagt tgcaatagag aaactaaata tcgttacacc
     2101 ataaccttta gggattagat agtgttcatc taatcccttt tattatgcta tgacataata
     2161 ttggcacatc tctgtgattg aagtgtaaat ttacacatgc tacaataagt attgcttata
     2221 gttttcttta ttttcagcac ctataaatgg tgcttttttg tacccgtaat tcattcaatc
     2281 actgacaact ggttctaagt attttatacg tacagcatac ttaaccatat accaaacgta
     2341 aactgtacat acagccatac gtataacgtt ttctatttgt atagaaaaga ggcggagcat
     2401 gtgaatgaaa ttctgaatca ggagaaatca atctatattc agataagcga aatgatagaa
     2461 aacgacatac tgcgggatgt gcttttggag gatgagcgcg tgccgagcac aaacgagctt
     2521 gcaaagctgt atgcgataaa tccgtctaca gcggcaaagg gcataaatat acttgtggat
     2581 gcaggaattt tgtataagaa aagagggatt ggaatgtttg tgagtgacgg agcaaaggaa
     2641 gtaataagga caaagcggaa agcccgtttt tgttgaagct gaacaatatg acgtagcgaa
     2701 aaaataaaaa ctagaaaatt ttcgtctaca tgacgaaaaa cagtaaaatt tttaatgttt
     2761 atcgaaaaat attctatagt gattcccgaa atatttttgt atactagcct taccaacaaa
     2821 acaaaggggg acaaaaatat atgaagaaaa aatttgttgc aatcatgatg gtagctgcta
     2881 tggcagcgtc aatggccgct tgtggatcag atgggggttc ttctggtaca cagaaagggg
     2941 gaagcagcac atcgacatct gatgttgcaa acaaggacaa gccacttgtg tggttcaacc
     3001 gtcagccatc aaacagctca acaggagagc ttgacacaac agcactcaac tacaacaagg
     3061 atacctatta tgtaggtttc gatgctaatc agggagctga acttcaggga gaaatggtaa
     3121 aagagtatat cgagaagaac attgatacga tcgacagaaa tggggatggc gttatcggat
     3181 atgtacttgc aatcggtgat atcggacaca acgattcaat tgcaagaaca agaggtgttc
     3241 gtaaagctct tggaacaggc gtagacaaga gcggcgagat tgacagtgct ccggcaggaa
     3301 caaactcaga cggaaaagca gctgaggttc aggacggcaa gattactgta aacggcaaag
     3361 actatgtagt aagagagctt gcttcacagg agatgaagaa ctcagcagga gctacatggg
     3421 atgcagctac agcaggaaat gcaatcggaa catggtcatc atcatttggc gagtcaatcg
     3481 acgtagttgt ttctaacaac gatggaatgg gaatgtcaat gttcaatgca tggtcaaagg
     3541 acaacaaggt tccaacattc ggatacgatg ccaacagtga tgcagtagca gctatcgcag
     3601 agggctatgg cggaacaatc agccagcatg ctgacgttca ggcttacctc acacttcgtg
     3661 tacttagaaa cgcactcgat ggtgtagata ttgatacagg tatcggcaca gaggatgatg
     3721 caggaaacgt attaagcgat gatgtatatg tttacaagga tgatgagaga tcttactacg
     3781 cattaaacgt agcagttaca gcagataact acaaggattt cacagattca acagttgtat
     3841 gggcacctgt ttcaacacag cttgattcag caaagcaccc tacaaagaag gtatggctga
     3901 atatctacaa tgcttcagat aacttcttaa gctctacata tcagccactc ttacagaagt
     3961 atgatgatct cttaaatctt gatgttgagt acatcggtgg tgatggacag acagagtcta
     4021 atatcacaaa cagacttgga aatccaagcc agtacgatgc attcgcaatc aacatggtta
     4081 agacagataa tgcagcttct tatacagctc ttttgaacca gtaaaagtat ttaaagcact
     4141 tagttgggga gggaaaaatc tctccccaaa ataaagtgta tacagacgac agcaaatata
     4201 gagttaggag caggaagtat gggcgataag aataatgaaa acatcttatc gattcgtggc
     4261 atgagcaagt catttggacg aaacagggtt cttgaccaca tcaatttaga tgtgaaaaaa
     4321 ggtactgtaa tgggtctcat gggggagaac ggagccggta agtcgacaat gatgaaatgt
     4381 ctttttggta cataccagaa ggacgaggga actatatatc tggacggaaa agaggtaagc
     4441 ttttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnaa tatgtcagta tcacagcgac agatgtgtga gattgcaaag gcaatatcat
     4681 acaactcaaa ggttatagta ttggatgagc ctacatcatc acttacggta caggaggttg
     4741 aaaagctgtt ccagatgatg aggatgcttc gtgaccaggg tatttcactt atctatattt
     4801 ctcacaagat ggatgagatt tttgagatat gtgatgagat ttcagttctt agagatggaa
     4861 atctggtcat gacaaagagc acaaaagaaa caaatatgaa tgaactgatt gcagcgatgg
     4921 ttggacgagt actggagaac agattccctc cggtagacaa caaaccaaag gatgtaatct
     4981 tgtcaataca gcatctttcg acaaagtacg agccacatct gcaggacatc acatttgatg
     5041 tgcatgaggg ggagatattt ggactatacg gacttgtcgg agcaggtcgt acagagcttc
     5101 ttgagacgat ttt
//
LOCUS       DPUF01000093            7688 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_22490, whole
            genome shotgun sequence.
ACCESSION   DPUF01000093 DPUF01000000
VERSION     DPUF01000093.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_22490"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="DHV81_03330"
     CDS             complement(<1..476)
                     /locus_tag="DHV81_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743114.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCI93545.1"
                     /translation="MAKKQTYDEKSIAILEGLEAVRKRPGMYIGSVTTKGLNHLIYEI
                     VDNSVDEHLAGACDTIRVTLEKNGSCTVEDNGRGIPVGMHAKGVSAARVVFSTLHAGG
                     KFDNSVYKTSGGLHGVGSSVVNALSTYMDVEISRDGYIHHDRYEKGVPVVELENGLL"
     gene            complement(515..1612)
                     /locus_tag="DHV81_03335"
     CDS             complement(515..1612)
                     /locus_tag="DHV81_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HCI93546.1"
                     /translation="MITNQKLKKSLEEIKNITSFDAILYSAKGKHLTGTVEEPEHSDI
                     FVKEFIVSEEDVREKNDAIAFAVEIDSELEYVLVMFCPYTSENLVIGRMAVCQLRTLV
                     LGESERYDRNNFIQNILLGNMLGSDIINRAKKLHIENRKRVVYVIDTGKNSNEIAVEL
                     VKNLADIRSGDFVVAMDEHNVVLVKDVEDIDSPKLQEKLSSIAGSLVDNLLAEAMIKV
                     RVGYGNPTDVLPKIAESYQEAKMALEVGRLFYVEKEIMAYDRLGIGRLIYQLPMSLCE
                     MFIREVFGDEVPQILDDEEAMSTISKFFENNLNISETARQLYVHRNTLVYRLERIEKS
                     IGLDIRTFDDAMIFKIATMVLAHIKDSRQGR"
     gene            complement(1815..2935)
                     /locus_tag="DHV81_03340"
                     /pseudo
     CDS             complement(1815..2935)
                     /locus_tag="DHV81_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527933.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     assembly_gap    2673..2687
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3151..3789)
                     /locus_tag="DHV81_03345"
     CDS             complement(3151..3789)
                     /locus_tag="DHV81_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCI93547.1"
                     /translation="MENRIVKFKSKESAGLNLHAIPGHFATSHSHINYYVDVTSIKTR
                     VSEAKEAAKALYTKVPHHSYIDTIVCMDGTEMIGAFLSQEIERNGLMSVNKHETIYVV
                     SPEINNGQMLFRDNNKGAIDGKHVVLLLATTTTGETIRRAMECINYYGGMVTCIASIF
                     STIDEVNGVKIDKLFDDDDVVNYQTYPVTECPFCKKGLPIEAMVNGFGYSKL"
     gene            complement(3843..4280)
                     /locus_tag="DHV81_03350"
     CDS             complement(3843..4280)
                     /locus_tag="DHV81_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCI93548.1"
                     /translation="MKINIKKLTTTAKIPTRGSEYAAGYDLYADITKELVIEPHETAK
                     IGTGLSIEIPDGYFGAVFARSGLAAKQALRPANCVGVCDSDYRGEYIVALHNDGTQAR
                     TINPGDRIAQLVVMPYLAVEFNEVSELSDTKRGAGGFGSTGAN"
     gene            complement(4352..5362)
                     /locus_tag="DHV81_03355"
     CDS             complement(4352..5362)
                     /locus_tag="DHV81_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCI93549.1"
                     /translation="MSRVGIIGAGSWGTALSLVLANNGHSVEIWSIVEKEIDMLKEKH
                     EHIDKLPGVKLPDSITFTTDIEETIKNNDILVLAVPSVFTRSTAKAMAPFVKDGQIIV
                     CVAKGIEENTLMTISDVVESEIPCADVAVMCGPSHAEEVGQLLPTTVVAGARTQKTAE
                     IVQDLFMNEVFRVYTSPDVLGMELGGSLKNVIALAAGMADGLGYGDNTKAALITRGIA
                     EISVLAIEMGAKAETLFGLTGIGDLIVTCESRHSRNRKAGMLIGQGYTMDEATKEVKM
                     VVEGIYSAKAALALAEKYDVRMPIIEEVNRVLFEDKPAKEAVSELMLRDRKIEHSSLE
                     WK"
     gene            complement(5445..6098)
                     /gene="plsY"
                     /locus_tag="DHV81_03360"
     CDS             complement(5445..6098)
                     /gene="plsY"
                     /locus_tag="DHV81_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-phosphate glycerol 3-phosphate
                     acyltransferase"
                     /protein_id="HCI93550.1"
                     /translation="MSKMILARILALVVGYFFGTFQTGYFYGKAHGIDVREHGSGNSG
                     TTNTLRTLGWKAGAVTFFGDLFKAITAVLIFYFIYRNTYPQAVKCIELYAGFGAVLGH
                     NFPFFLSFKGGKGIACTSGVILAVCPIAAPICLVLFILAVAITRYVSLGSILVVISFL
                     IQAIVFNHMGLLGVSGNYAVEFDILAACFTAMAIWRHRANIKRLLSGTENKFGQKAK"
     gene            complement(6091..7313)
                     /locus_tag="DHV81_03365"
                     /pseudo
     CDS             complement(6091..7313)
                     /locus_tag="DHV81_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080090.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
     assembly_gap    6862..6951
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7314..7430
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2077 a         1844 c         1202 g         2343 t
ORIGIN      
        1 agcaggccat tttcaagctc cacgacagga acaccctttt catatctgtc atggtggata
       61 tagccatctc tgcttatctc cacatccata tatgtggata atgcatttac aaccgatgaa
      121 ccaactccgt gcagacctcc actggtctta tatacagaat tgtcgaattt acctcctgcg
      181 tgcagtgtcg agaatacaac acgcgccgca gatacaccct tagcatgcat acctacaggt
      241 attccacgtc cgttatcctc caccgtacac gaaccgtttt tctcaagagt tacccttatg
      301 gtatcacagg cacctgccag atgctcatct acagagttgt ccacaatctc atatatcaga
      361 tggtttagtc ccttggttgt gacacttcct atatacatac ccggtctttt tctgactgcc
      421 tcaagtcctt ctaaaattgc tatgctcttc tcatcgtatg tttgcttctt ggccatactt
      481 gtctcctact tctttaaatt gcgtaataat tatattatct gccctgcctg ctatccttga
      541 tatgcgctaa caccatcgtt gctattttaa atatcatagc atcatcaaag gttctgatat
      601 caagacctat cgacttctca atacgctcta gcctgtacac aagcgtattt ctatgcacat
      661 aaagctgtct tgcagtctct gaaatgttca gattgttctc gaagaacttg gagattgtag
      721 acattgcctc ctcgtcatca agtatctgtg gcacctcatc accaaaaacc tcacggataa
      781 acatctcaca cagactcatc ggaagctgat aaataagtct tcctatgccc agcctgtcat
      841 atgccataat ttccttttcc acgtaaaaca gcctgccgac ctcaagcgcc atttttgcct
      901 cctgataaga ctctgcaatc tttggaagaa catccgtcgg attgccatag cccacccgta
      961 ccttaatcat agcctctgca agcagattgt caaccagact tcctgcaata gatgaaagct
     1021 tttcctgaag ctttggtgaa tctatatcct caacatcttt taccagcact acattatgct
     1081 catccatcgc caccacaaaa tcaccgcttc tgatatctgc cagattcttt acaagctcaa
     1141 ctgctatctc attgctgttc tttccggtat ctataacata tactactcgt tttctgtttt
     1201 ctatatgtaa tttcttggca cggtttatta tatcagaacc aagcatattg ccaagaagaa
     1261 tattctgaat aaaattattt ctgtcatatc gctctgactc accgagcaca agtgtacgaa
     1321 gctgacaaac tgccatcctg cctattacaa gattctcact cgtatacggg cagaacatta
     1381 cgagcacata ctcaagctct gaatcaattt caaccgcaaa tgcgattgca tcgttttttt
     1441 ccctaacatc ctcttcggat actataaact ccttcacaaa aatgtcgcta tgttccggct
     1501 cttccaccgt acctgtcaga tgctttccct tggctgagta taaaatcgca tcaaacgaag
     1561 taatattttt tatttcctca agtgattttt tcagtttctg attcgtaatc atattcttat
     1621 cctctcatta ctgttctgac gcaggcaagt aaaaagcctc catgaggtaa actctgtaca
     1681 cctgctgtag tttaacacca taacttaact attcagaacc atacattcgc gtcagaacct
     1741 aacatttgcg aaagaaaacc gggtgtaaat tatacacccg gtctgtcaat ttacaactat
     1801 ttaattttaa cgaactagtt ggtaattgtc aattcagttt ctttatcaaa tacatgaatc
     1861 ttgtttggat caaatgcaag ctcaatgtga tcaccgtaac gagccggtgt atctgaatca
     1921 actcttgctg acatatttgc gcctgcaact gtgaagtata ataatacctc tgcaccaagt
     1981 aactcatatc ctgtaacatc tgaatcaatc ttgcatgtag caaatttatt aagatgctct
     2041 gctgaatcgt agatatcctc aggtctgata ccgaatacta cgttctttcc tgcataaccg
     2101 ccatcgataa gcttcttagc ctttgcctct ggaagctcaa cctggtattt atcgaattta
     2161 aggaatgcct ttgctccctc aacaacacac tgagcatcta caaagttcat ctgtggtgat
     2221 ccgatgaatc ctgcaacgaa caggttgttt ggctgattgt aaagcttctg tgggctgtct
     2281 acctgcatga taacaccatc tttaagaaca acgattcttg ttccaagtgt catagcctct
     2341 gtctgatcat gtgtaacgta gatgattgtt gcacctaatc tgttatgtaa tgaagaaatc
     2401 tcagatctca tctgtacacg aagctttgca tcaaggtttg ataaaggctc atccataagg
     2461 aataccttag gattacgaac gattgcacga cccatggcaa ctcgctgtct ctgtccacct
     2521 gaaagagcct ttggcttacg gtctaataat ttctcaaggt caaggattct tgctgcctcc
     2581 tcaacttttg ccttaatctc atcctttgga acttttctaa gcttaagtcc aaatgccatg
     2641 ttgtcaaata ctgtcatatg tgggtaaaga gcnnnnnnnn nnnnnnntgg aataccattg
     2701 cgatatcacg atcctttggc tctacgtcat tcataacctt tccgtcgatg ataagctctc
     2761 ctgaagaaat ctcctcaaga cctgcgatca tacgaagtgt tgttgatttt ccacatcctg
     2821 aaggtcctac gaagatgatg aactctttat cttctacttc aaggttgaaa tccttaactg
     2881 cctcaaatcc gtttggataa actttgcaaa tgttctttaa tgataaactt gccattttaa
     2941 aaatacctcc taagtattat gtacatatat gtaactgttc agtgccatga ctgtctgtta
     3001 ccttgtttct ttgtatcttt ataatacaaa acatatcgtc aattggctat agtcaatccg
     3061 ccaaataatt taacgtgttg ttgtcattat gacgaaacac agtctgatat cgaaacaatt
     3121 acaaggcaat tactgcaata aacgtactaa ttacagctta gaatatccaa agccattgac
     3181 catagcctca atcggcagcc ccttcttgca gaatggacac tccgtaacag gatatgtctg
     3241 gtagtttacc acatcatcgt catcaaacag cttatcaatc tttactccat tgacctcatc
     3301 tatggttgag aatatggacg caatacatgt gaccataccg ccataatagt tgatacactc
     3361 catcgctctt ctgattgtct cacctgttgt ggtagttgcc agaagaagta caacatgctt
     3421 gccatctata gcgcccttat tgttatccct gaaaagcatc tgtccgttgt taatctcagg
     3481 cgatacaacg taaattgtct cgtgcttatt cacagacata agtccgtttc tctcaatctc
     3541 ctggcttaag aatgcaccaa tcatttctgt tccatccata catacgattg tatctatata
     3601 tgaatgatgc ggtaccttgg tgtaaagagc ctttgcagct tcctttgcct cggacacacg
     3661 ggtcttgatg cttgtcacat ctacataata attaatatgc gaatgacttg tagcgaaatg
     3721 tcccggtata gcatgtaagt ttaagcctgc actctccttt gatttaaatt ttacaatacg
     3781 attttccatg ataagtcccc ctttttaagt aaagattagt tttaattgta actcatttgt
     3841 gattaattag ctcctgtaga accaaatcca cctgcaccac gcttcgtatc agaaagctct
     3901 gacacctcat taaactcaac agccagatat ggcatgacca caagctgcgc tatacgatct
     3961 ccgggattaa tagtgcgtgc ctgcgttcca tcattgtgaa gagctacaat atactctccc
     4021 ctgtaatcac tgtcacacac acccacgcag tttgccgggc gaagtgcctg ctttgcagct
     4081 aaaccacttc tggcaaaaac tgccccaaaa tatccgtcag gaatctctat cgaaagcccc
     4141 gtgcctatct ttgctgtctc atgtggctca atgacaagct ccttagtaat gtcggcatat
     4201 aaatcatagc ctgctgcata ttcacttccc cttgtaggga ttttggctgt ggttgtcaat
     4261 ttttttatat ttattttcat tttataattt cccctgatgt aattttatat atgaccgtct
     4321 atagccattt attacccaac ctgttaacta attatttcca ctccagactt gagtgctcaa
     4381 tcttcctgtc acgaagcata agctctgaga cagcctcctt tgccggctta tcctcaaaaa
     4441 gcactctgtt tacttcctcg ataatcggca tccttacatc atatttttct gcaagggcaa
     4501 gtgcagcctt tgctgaatag atgccctcta caaccatctt gacttctttt gttgcttcat
     4561 ccattgtgta tccctggcct ataagcattc cggcctttct attacgactg tgacgactct
     4621 cacatgtaac aataaggtct ccgatacctg tcaatccaaa aagggtttct gcctttgcac
     4681 ccatctcaat cgcaagcacg gatatctctg caattcctct tgtgataagt gctgcctttg
     4741 tattatctcc atagccaagt ccgtctgcca tacctgctgc aagtgctatg acattcttga
     4801 gggagcctcc aagctccatt ccaagcacat caggactggt atacacacga aatacctcat
     4861 tcataaaaag atcctgtact atctcagcgg tcttctgtgt ccttgctcct gcaacaacag
     4921 tcgtaggaag cagctgtccg acctcctctg catgtgaagg tccgcacatc acagccacat
     4981 cagcgcaagg tatctcactc tctaccacat cagatatagt catcagagta ttttcttcaa
     5041 ttccctttgc cacacagaca attatctgcc catccttcac aaaaggagcc atcgcttttg
     5101 ctgtgcttct ggtaaaaaca gacggtaccg caagcacaag tatatcgtta tttttgattg
     5161 tttcctcaat atctgttgta aaggtaatgc tgtccggaag ctttactcca ggaagcttat
     5221 caatatgctc atgcttttct ttaagcatat caatctcttt ctccacgatt gaccatattt
     5281 caacagaatg tccgttattt gcaagcacca gggataatgc tgttccccag cttcccgcac
     5341 ctataatacc aactctactc attttttatt cctcatacta tatataatga tatgagggtc
     5401 aaaaggtttt ttgcccctca tttattgtaa tccaaaaata ttatttactt tgccttctgg
     5461 ccaaatttat tttcagttcc gcttaatagt cttttgatat ttgctctgtg acgccagata
     5521 gccatcgccg taaaacaagc tgcaagaata tcaaactcaa ccgcataatt gccgctcact
     5581 ccaagcagcc ccatatggtt aaacacaatt gcctgaatca gaaatgatat aacaaccagt
     5641 atagaaccaa gtgagacata tcttgttatg gctactgcaa gaataaacaa taccagacat
     5701 atcggagccg ctatcggaca tacagcaagt attacacctg atgtacatgc tattcccttt
     5761 cctcccttaa aactaaggaa gaacggaaaa ttgtgtccaa gcaccgctcc aaagcctgca
     5821 taaagctcta tgcatttaac agcttgcggg tatgtatttc tatatatgaa ataaaatatc
     5881 aaaactgctg tgattgcctt gaacaaatca ccaaagaagg taactgcacc ggccttccat
     5941 ccaagtgtac gaagcgtatt ggtggtgcct gaattgccgc ttccatgctc cctcacatct
     6001 attccatgtg ccttaccata aaaatatccg gtctgaaagg ttccaaagaa ataccctacg
     6061 accaatgcta aaatcctagc cagtatcatt ttactcattt tcctttcgtt ctctcgtaat
     6121 aaacttaagt gcagttcccc tgaaaccaaa tgtatccctg attctgttct cgaggtatct
     6181 ggtatatgag aaatgcataa gattcttgtc atttacaaag attacaaagg taggcggctt
     6241 aacagaaacc tgtgtcacat agtatatctt tagtctcttg cccttgtctg taggtggctg
     6301 ctgcatagca actgcctccg taactatctc atttaacact cctgttgcaa cacgcattga
     6361 gttattttct ataacaacat caatgagctc aaatatcttg ttgagtctct gacctgactt
     6421 tgccgaaata aacagtatct ctgcatatgg aataaagctt aatatctgtc tgattttctc
     6481 tgtgtgctgc tttacagtat tattatcctt ttcaatggca tcccatttat tgacagcaat
     6541 gataattccc ttgcctcttt cgtgggcaat gcccgcaatt ttagcatcct gctctgtaac
     6601 accctcagta gcatctatca ctactatgac aacatcagct ctttccactg cagaaactgc
     6661 acgtataata ctgtatcgct ctatatcctc ttttatcttg ttcttgcgcc tgagaccggc
     6721 tgtatcgata aatatatatt ccctgccatc atactttata gctgtatcga tagcatctct
     6781 tgtggtgcct gctatatcag acactatcac cctgtcctcg cgggcaagct tgttgataag
     6841 tgatgactta cctacattag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaatgcttc
     6961 acaacctcat cgagcatatc tccgagtccc agcatcgaag ctgctgagat aggatgcgga
     7021 tcacctattc ctaagttata aaactcatac acatccgtca tgtacttttc aaagctatcc
     7081 accttattga caaccaatac aaccggcttg tgtgaacggc gaagcatatc tgccaccttt
     7141 gaatctgaat caacaagccc ctgcttcaca tctgtgataa aaataataac atctgctgtg
     7201 tcgatggcaa tctgtgcctg ctctctcatc tgcgacagta tcacatctcc tgattccggc
     7261 tcaataccac ctgtatcgat catggtaaaa ttgtaatcaa gccatgatac atcnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atggccacta
     7441 ctggtctgct catatactct cctgttctaa attcatatta gcctatagaa taattgtaac
     7501 tatttagaat aattataaaa tgatatcttt taaccattta atcataaaat aataaacaaa
     7561 acatctataa cagagtaatt atatcacaat accatttaca gagacaacta ttcatatgtc
     7621 aaaagtggtt cttttgatga aattaatcca ttttctttaa gaacagctat cagattagta
     7681 cagttttc
//
LOCUS       DPUF01000094            3553 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_31804, whole
            genome shotgun sequence.
ACCESSION   DPUF01000094 DPUF01000000
VERSION     DPUF01000094.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_31804"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="DHV81_03370"
     CDS             complement(<1..500)
                     /locus_tag="DHV81_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCI93551.1"
                     /translation="MTIGIIVVNIIVFVLMSISGSTLDAQYMAAHGAMYPEYIKDGQY
                     WRLFTSMFMHFGLMHILNNMVVLGAVGQIVEKAMGHVKLLITFLVSGMCGSVLSYIVM
                     LYNNDFAVSAGASGAIFGLVGALVWIVIANRGFYEGISRQQAVFMVILMIYYGVSTQG
                     VDNWAHG"
     gene            complement(613..1806)
                     /locus_tag="DHV81_03375"
     CDS             complement(613..1806)
                     /locus_tag="DHV81_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HCI93552.1"
                     /translation="MNSNEIIYERKMTGSFMKLHCDEVKQLDEKILLKNNIPGFLKME
                     KCYVNNEGQYWYDISGVQSLEMRCQYNDIKLEFLEKLVVSICNKMELLENHLVGTDCL
                     ALDPQLIHIKGVNQDEEIYFTAYPAQMKTMCTTFQHLMEYMLTKLDHSDAEAIHIAYG
                     IYEKTINGNYSIADIRNAIIEERQKKVAEKPVEEMVAPISQPQVRVEEPKEQIKPTLY
                     DKLRVYLLEQLDIRLPERKKPESVTEKHKKPDTDRKWFKKEKRKENREKVDDTMLIHP
                     SPEAAPEPVPRQIHPTVCLSDYREHPQGMLLYEGMENRSNIIIDKDCTRIGQGDEADA
                     VIGKDTISHFHAVINRENQEFYLEDLNSTNGTFVNNEVLAYKQKKQLKSNDIIRFADV
                     KYRFV"
     gene            complement(1796..2032)
                     /locus_tag="DHV81_03380"
     CDS             complement(1796..2032)
                     /locus_tag="DHV81_03380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93553.1"
                     /translation="MTGNKLNIDLSYGLKGSYTVEGAIIIPIFIVMLAIAMKMGLVLY
                     NEMKADSSYEYATDMWLVDDFYNYQVLKEVVNEF"
     gene            complement(2029..2436)
                     /locus_tag="DHV81_03385"
     CDS             complement(2029..2436)
                     /locus_tag="DHV81_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCI93554.1"
                     /translation="MIQLIILLGIISIIDFSTKKIPVVVLVVMGIVGIASSFYAKQSI
                     ISVAVALIPGIVLMAAAFLTGEQIGYGDAAVVTLMGLFVTADIVCSTMVMGLTIAGTI
                     SVILIASGRAGRKKQIAFTPFLLLGYGLTEVFM"
     gene            complement(2433..3281)
                     /locus_tag="DHV81_03390"
     CDS             complement(2433..3281)
                     /locus_tag="DHV81_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93555.1"
                     /translation="MKVSFVNKVKFHIKKLYRKAKLPGDDSSQKMPSCTCLKASYTLE
                     AAVIFPLVAGFFVFILFFFRVVQVESQVKAALYYSSRMSALASSANDSSVVSVATAEV
                     LFRSQISDSKHIDTYVSGGRYGVSLLGSSMGGDDVSLKAKYKVKLPVSFFTVDGIWIE
                     DYSNSRKWTGKNPGEKTDPYVFYTDYGSVYHLSEQCNYLDLSIKSIKWSQVGASRNKD
                     GRKYHACYCAADKKTEGSTVFITDYGTNYHGKLGCSKLKRTVHKVHKSDVDGKNLCSK
                     CKGEGT"
     gene            complement(3315..>3553)
                     /locus_tag="DHV81_03395"
     CDS             complement(3315..>3553)
                     /locus_tag="DHV81_03395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93556.1"
                     /translation="LFTKSMKNISYRSMDVQEATVRMTSGHESFRMDNAICELSADVS
                     YKYHGTFLCFVNLLDGADNEYTIKNKAKYSYYGR"
BASE COUNT          953 a          902 c          567 g         1131 t
ORIGIN      
        1 ccatgagccc aattgtccac tccctgcgtt gaaactccat agtatatcat cagtataacc
       61 ataaatacag cctgctgtct gctgatgccc tcataaaagc ctctgtttgc tataactatc
      121 cacacaagtg ctcccaccag accaaatatc gccccggagg cgcctgccga taccgcaaag
      181 tcattattat aaagcataac gatgtacgat aatacacttc cacacatgcc tgacacaaga
      241 aatgtgatca gaagcttcac atgccccatg gctttctcga ctatctgacc gacagcacct
      301 aaaaccacca tattgttaag tatatgcatc aatccaaaat gcataaacat cgaagtgaac
      361 agcctccagt actggccatc ctttatatac tcagggtaca tggctccgtg ggcagccata
      421 tactgtgcat caagtgtgct gcctgatata ctcatcagca caaaaactat tatatttacc
      481 actattatgc caattgtcat atatggttta tcataaaatc tttttatatt ctgcaaatct
      541 gtggggtctc cttaattttc ctgttaaata cttttttctt attataaatt ttaaggagct
      601 tatttgcaac aattatacaa accggtactt aacatcggca aatctgatga tatcatttga
      661 cttgagctgc ttcttctgct tgtacgcaag cacctcatta ttcacaaagg ttccatttgt
      721 ggagtttaaa tcctccagat aaaattcctg gttttcccta tttataactg catgaaaatg
      781 acttattgta tccttgccta ttactgcatc ggcctcatcg ccctgaccaa tacgcgtaca
      841 atctttgtct attattatat tactgcggtt ttccatgccc tcatacaaaa gcatgccctg
      901 tgggtgctcc ctgtaatcgc tcagacatac tgtggggtgt atctgacgcg gtacaggctc
      961 cggtgcagcc tctgggctag ggtgtataag cattgtgtca tctacctttt cacgattttc
     1021 ttttcgtttt tcttttttaa accattttct gtctgtgtcc ggttttttat gcttttctgt
     1081 cacggattcc ggcttctttc gctccggcag cctgatatcc agctgctcca gcagatacac
     1141 acgaagctta tcgtacagtg taggctttat ctgctccttc ggctcctcta ccctgacctg
     1201 tggctgtgaa atcggcgcaa ccatctcctc caccggcttt tctgccacct ttttctgcct
     1261 ctcctcgatt atggcatttc gaatatcagc gatactgtaa ttgccattta tggttttctc
     1321 gtatatgcca tatgctatat gtatcgcttc agcatcactg tggtcaagtt tcgttagcat
     1381 atactccatt agatgttgga atgtagtaca catagtcttc atctgtgccg ggtatgctgt
     1441 gaaatatatt tcctcatcct gatttacccc ttttatgtgt attagctgag gatcgagtgc
     1501 caggcagtcc gtgcctacaa gatgattctc caaaagctcc atcttgttac agatactcac
     1561 cacaagcttt tcaagaaact caagctttat atcattatac tggcagcgca tttccaaaga
     1621 ctgaactccg gatatatcat accagtactg cccttcatta ttcacataac acttctccat
     1681 cttcagaaag cccggaatat tgtttttcaa caatatcttt tcatctaact gcttcacctc
     1741 gtcacaatgc agcttcataa agctgcctgt catctttctt tcatatatta tttcattaga
     1801 attcatttac aacctctttc aaaacctgat aattatagaa atcatccacc agccacatat
     1861 cagtagcata ctcataagag ctgtctgctt tcatttcgtt atacagcaca agtcccatct
     1921 tcatcgctat cgcaagcata acaataaata taggtataat aattgctccc tctactgtat
     1981 aactgccttt tagtccataa gataaatcta tatttaattt atttccagtc acataaatac
     2041 ctccgtcaat ccatatccca gcagtagaaa tggtgtaaat gcaatctgct tctttcttcc
     2101 agccctgcct gatgcaatta aaataactga gattgttccc gctattgtca gtcccatcac
     2161 catagtgctg cacactatgt cagcagtcac aaacaatccc ataagcgtca caacagcagc
     2221 atctccatag cctatctgct ctcctgtaag aaacgcagct gccataagca ctataccggg
     2281 aatcagggca acagccactg atataattga ctgcttcgca taaaaagagc ttgcaatacc
     2341 tacaatcccc attactacca atacaactac cggtatcttt ttggttgaaa agtcaattat
     2401 actgattatt ccaagtaaaa tgataagctg tatcatgttc cttctccttt acatttactg
     2461 cacagatttt tgccatctac atccgactta tgcaccttgt gtactgtccg cttaagctta
     2521 ctgcatccaa gctttccgtg atagttcgtg ccataatctg ttataaatac cgttgaacct
     2581 tctgtttttt tatccgcggc acagtagcat gcgtggtatt tcctaccatc tttgtttctg
     2641 cttgccccca cctgtgacca tttgatgctc tttatactta aatccaggta gttgcactgc
     2701 tcggacaggt gataaacact gccatagtca gtgtaaaata catatgggtc cgtcttttcg
     2761 ccgggatttt ttcctgtcca ttttctgctg ttgctataat cctctatcca tattccgtcc
     2821 acagtaaaaa agctcaccgg aagcttaacc ttatactttg ccttaagcga gacatcatca
     2881 ccacccatac tcgagccaag cagtgacact ccataccttc caccgctcac gtatgtatca
     2941 atgtgcttcg aatcagatat ttggctcctg aatagtacct ctgcagtggc cactgataca
     3001 actgacgaat cgtttgcact gctcgctaat gcactcatcc tggatgaata atacagagca
     3061 gccttcacct gcgactccac ctgaaccacc ctgaagaaaa aaagtataaa tacaaaaaag
     3121 cctgctacaa gcggaaatat taccgcggcc tcaagtgtat atgacgcttt caggcaggtg
     3181 catgagggca tcttttgaga actatcatcg cctgggagtt tggcctttct ataaagtttt
     3241 tttatgtgga actttacttt attaacaaaa gacaccttca tacacctgcc tttcttattt
     3301 tccttcttat ttagtcagcg tccataatag gaatactttg ccttattttt aatggtatac
     3361 tcgttatccg ccccatctaa cagattcaca aagcataaaa atgtgccatg atatttgtag
     3421 cttacatctg cactaagctc acaaattgca ttgtccattc taaagctttc atgaccactg
     3481 gtcatacgta ctgtagcctc ctgcacatcc atcgagcgat acgatatatt tttcatgctc
     3541 tttgtaaaaa gca
//
LOCUS       DPUF01000095            3216 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_18665, whole
            genome shotgun sequence.
ACCESSION   DPUF01000095 DPUF01000000
VERSION     DPUF01000095.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_18665"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="DHV81_03400"
     CDS             complement(<1..581)
                     /locus_tag="DHV81_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI93557.1"
                     /translation="MNLSQLQYFKVLAQEEHYTRAAQMLSITQPSLSHAISQLEQELG
                     TRLFEKKGRNVVLTRYGKMFLPYVEESLKVLNEGVQRIQEVNGSRHGIIHLAYIYTMG
                     SEFAPKLVRKFLDAYPDYDIEFHFTVGTTGEIIDGLKDDKYDIVFSSYKDGEQDIDFK
                     KIANQKLVLAVPMDHPLSIKDSVDLRDTIEYPQ"
     gene            1170..3170
                     /locus_tag="DHV81_03405"
     CDS             1170..3170
                     /locus_tag="DHV81_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCI93558.1"
                     /translation="MKNKYPHIFEPITVRRMTVKNRIVMTPMGTNYGEQNGEMSFLHI
                     NYYEQRAKGGTGLIIVENASVDSPQGSNGTTQLRIDLDNYIPRLFKLCESVHKHGACI
                     AIQLNHAGASAMSSRIGMQPVSASDVPSKAGGEIPRPLEKDEIMHIVKKYGEAAKRAQ
                     ICGFDAVEIHAGHSYLISQFLSPITNKRTDEFGGSAENRARFAKLVIEEVRKQVGPFF
                     PIFVRISADELMEGGNTLEDTLEYLKYFEKEVDVFDVSCGLNGSIQYQIDANYLPDGW
                     RSFMAKAVKEKYNKPCITVGNIRDPQVAEDILAGGDADFIGMGRGLIADPEWVNKVEF
                     GNVCDIRKCISCNIGCAGHRIGLNQPIRCTVNPAVNSGEDYMKTKVNKPCNVVVIGGG
                     TAGLEAACTAAEVGCTTFLIEKKAELGGLASVISKIPDKKRLADFPNYMIHRASKLHN
                     LFVFKNTSATVDMVKALNPDIIVNATGSVPTLPPITGLHDLVDKDGTNVATVLKMIER
                     INEYPEDMKGQKIAIIGGGAVGLDVMEFFTERGAEVTMVEMLPMIGNGLDPVTKCDTN
                     AKMAKYGVKQMTNTALQEVKNDRFIVKNPEGEIEEIPFDYGFICLGMRANTPVLSEID
                     EAFSNTNVEIVNIGDSKRARRIIEGTEEGRNILNVLARHDYL"
BASE COUNT          960 a          722 c          707 g          827 t
ORIGIN      
        1 atctgcggat actctattgt gtcccgcaaa tccacactgt cctttatgga aagtgggtga
       61 tccattggca ctgcaagcac aagcttctga ttcgcgatct tcttaaaatc tatatcctgt
      121 tccccatcct tataagaaga aaaaactatg tcatatttgt catcctttag tccatctata
      181 atctctccgg ttgttccaac cgtaaaatga aattctatat cataatccgg gtatgcatcg
      241 agaaatttac gcaccagctt tggcgcgaac tcacttccca tcgtatatat atatgccaga
      301 tgtatgattc catgcctgct gccattgacc tcctgaatgc gctgcacacc ctcattaagc
      361 acctttaatg attcctcaac atacggcaga aacatcttcc catatctggt cagcacaaca
      421 tttcttccct ttttttcaaa caaacgcgtg ccaagctcct gctctagctg tgagattgca
      481 tggcttaaag acggctgcgt aatcgacagc atctgtgccg ccctcgtata atgttcttcc
      541 tgcgccagta ccttgaagta ctgcaattga ctcagattca tagtacaccc cctgtgtatt
      601 tatatattta ttcgccggag gttcgcttgc cctgcgctgc acaccggtgg tatatgctcg
      661 atttatgacg tgtcgtgtgg gagttatatg ccgcctcaag caaaagtggc gcggaaaata
      721 ctcgttcaag caacacagcc tttagtatac cagtaaagta aaaagcgcgt ttgatttttc
      781 gcgcctaata aactctgttt gaggcggcat ttactcccac tagactaagg aattatgaga
      841 gcatatacca ccggtgtgca gcgcatggca agcgaacctc cggcaaatgc tcacaacaaa
      901 gtctagaaat aatatacacc atttgataga caaaaactat agatttcgta taaaagatgc
      961 attggacatt tatacatttt tgctgtacct tgaatgtaac aagagtgcat tgcacttttt
     1021 gattgttaaa aaaatcacat actttgtata tcgcagcctt tgataacaaa tattgacaat
     1081 cagtttaatg aaacaaaact ataatgtaat gagagcaatt tacactaccg cgtgcccacc
     1141 cggttagcag attaaggaga aaaaatacta tgaaaaacaa atatccacat atttttgagc
     1201 caattacagt acgcagaatg acagtaaaga accgtatcgt catgactcca atgggaacta
     1261 actacggtga gcagaacgga gaaatgagct ttctccacat caactactat gagcagagag
     1321 caaagggcgg cactggtctc atcatcgttg agaatgcaag cgttgactct ccgcagggtt
     1381 caaacggaac aacacagctt cgtattgatc tggacaatta catcccaaga cttttcaagc
     1441 tctgcgagag tgttcacaag catggtgcat gtatcgcaat acagttaaat cacgccggtg
     1501 cttctgcaat gagctcacgt atcggaatgc agcctgtatc agcatcagac gtaccttcaa
     1561 aggcaggcgg agaaatccca aggcctctcg agaaggatga aatcatgcat atcgtaaaga
     1621 aatacggtga ggcagccaag cgtgcccaga tctgcggctt cgacgctgtt gaaatccacg
     1681 caggacactc ttaccttatc agtcagttcc tttcacctat cacaaacaag agaactgatg
     1741 agttcggcgg ctctgcagag aaccgtgcaa gatttgcaaa gctggttatc gaggaggttc
     1801 gtaagcaggt tggacctttc ttccctatct ttgtccgtat cagtgcagac gagctcatgg
     1861 agggtggaaa cacacttgag gatacacttg agtacttaaa gtattttgag aaggaagtag
     1921 atgtattcga cgtatcatgt ggtctcaacg gctctatcca gtaccagatt gatgcaaact
     1981 acttacctga cggctggcgt tcattcatgg caaaggctgt aaaagagaaa tacaataagc
     2041 catgtatcac agtaggaaat atccgtgacc cacaggtagc cgaggatatc cttgcaggag
     2101 gcgatgctga tttcatcggt atgggccgtg gacttatcgc tgaccctgag tgggttaata
     2161 aggtagagtt cggaaatgtg tgcgatatca gaaaatgtat ctcatgtaac attggatgcg
     2221 ccggacacag aatcggtctc aaccagccaa tccgctgtac agtaaaccct gccgtaaaca
     2281 gcggtgagga ttacatgaag acaaaggtaa ataagccttg caacgtggtt gtaatcggtg
     2341 gtggtactgc cggtcttgag gcagcctgca cagcagcaga ggttggatgt acaaccttcc
     2401 tcattgagaa gaaggcagag cttggcggac ttgcatcagt tatctccaag attcctgaca
     2461 agaagcgtct tgcagacttc cctaactaca tgattcaccg tgcaagcaag cttcacaacc
     2521 tgtttgtctt caaaaataca tcagcaacag ttgatatggt aaaggcctta aaccctgata
     2581 tcatagtaaa cgcaacaggc tctgtaccta ctctcccacc tatcacaggt cttcacgatc
     2641 tcgttgacaa ggatggcaca aatgttgcaa cagtccttaa gatgattgag cgcatcaatg
     2701 agtatcctga ggatatgaag ggacagaaaa tcgccatcat cggcggtggt gccgtaggtc
     2761 ttgacgtgat ggaattcttc acagaaagag gcgcagaggt aacaatggtt gagatgcttc
     2821 caatgattgg aaacggactt gaccctgtca caaagtgtga cacaaacgca aaaatggcaa
     2881 aatacggtgt aaaacagatg accaacactg ctctgcagga ggttaagaat gaccgtttca
     2941 tcgtaaagaa tccagagggt gagattgagg aaatcccatt tgattacgga ttcatctgcc
     3001 ttggtatgag agccaacaca cctgttcttt cagagattga tgaggctttc agtaatacca
     3061 atgttgaaat cgtaaatatc ggagacagca agcgcgcccg cagaatcatt gagggtacag
     3121 aggaaggtag aaatatctta aacgtgcttg cccgtcacga ttatttataa attctccttt
     3181 aaaataataa acctataaat cgagtaggag gcggtg
//
LOCUS       DPUF01000096           14207 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6915, whole
            genome shotgun sequence.
ACCESSION   DPUF01000096 DPUF01000000
VERSION     DPUF01000096.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 14207)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14207)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14207
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6915"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            250..1677
                     /locus_tag="DHV81_03410"
                     /pseudo
     CDS             250..1677
                     /locus_tag="DHV81_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741239.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     assembly_gap    1002..1113
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1830..2777
                     /locus_tag="DHV81_03415"
                     /pseudo
     CDS             1830..2777
                     /locus_tag="DHV81_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517723.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
     assembly_gap    2258..2384
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2987..3268
                     /locus_tag="DHV81_03420"
     CDS             2987..3268
                     /locus_tag="DHV81_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI93559.1"
                     /translation="MISLYQKTWTEINNDIAQKIVRLRKRKKITQKQLAARSGVSLGS
                     LKRFEQSGEISLQSLTKIAIALDVENELEDLFNNVPFASIEEVINEQTK"
     gene            3252..3835
                     /locus_tag="DHV81_03425"
                     /pseudo
     CDS             3252..3835
                     /locus_tag="DHV81_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005971903.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4090..5001
                     /locus_tag="DHV81_03430"
     CDS             4090..5001
                     /locus_tag="DHV81_03430"
                     /inference="COORDINATES: protein motif:HMM:PF01381.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93560.1"
                     /translation="MESNTLGRILRENREKQKLSKQKVCRGLCTVMALSRYESDERIP
                     DKFLMDALLERLGFQPSKYEFVASDQEFEYSMKRAQIEKLLYEKRDVQACREALQEYE
                     KQVQEKERLHRQYILLKKGLILGREEKYSDAINVFEQALKCTECSETNVANQTDILLT
                     NTETELLYLIGKFLYIDGKKKDACTYFRMLKNQMEQRPAGKEKWKEYYPCILYRLAQC
                     EFEAYNYGASYELILQAEKVLIENYEYDCLYEVLELKEIICARLGIAKKTNENLQLAL
                     KLMAMGENGKFTKEGLNLWENTASQQL"
     gene            4971..5885
                     /locus_tag="DHV81_03435"
     CDS             4971..5885
                     /locus_tag="DHV81_03435"
                     /inference="COORDINATES: protein motif:HMM:PF01381.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93561.1"
                     /translation="MGEYSKSAIIKYTRKSLGITQEELSESVCDPVTLSRYESGKIDP
                     SDSKFLRLMEKMGEKGSTFIFQLETSLLEVEDESEKLNKAIEQHDWEEAERIKNNLIQ
                     NNNFNLDYFENRQYLQWVETIIQYEQGMINERQVIEKLEEAWSYTCGNINTEEFNMNR
                     IYRETEIIILHEIAIFYKLLGKLDKACVFYENLIKYFERADMVNDTKPIYLIYIGYSN
                     ILGVMGEHEKSMEICFKAIKRGLAENKMNYLYNFYYNIGCSLQIKGNDEEKRLAKLYI
                     WTAYQLCQAYPENKRNLEIIKRRYEQFD"
     gene            6248..7648
                     /locus_tag="DHV81_03440"
     CDS             6248..7648
                     /locus_tag="DHV81_03440"
                     /inference="COORDINATES: protein
                     motif:HMM:PF04055.19,HMM:TIGR04085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93562.1"
                     /translation="MQKDKLVLEDYETFSVRGKNRIVIFPNVPFWIAVTETGFKALEE
                     MRKNGDKVKVAQKLYGKSEKEQVEEINKFFLPLEESNVLHSTLKGNVVTNRKVLKPNK
                     ITFLQTMRCNLRCRHCCVADMPNQNLESMSLDKAKKALDRCLLIMDEKEKGLSFLGGE
                     PLCGEKFPELVEYARSLGFKVGLSTNGTLVDEAFARMAKKNNINVQISLDGTDRESHE
                     FVRGKGTWDSVISAIRLLNKYCVDIQTNLVYHRGNINKLEEYFDFAINNNIKKVRLIS
                     LMNMGRAVGQMERVPLDEFVDIMYKLIKKRPDIIDVLDETSFMGLVMGARFSQKMVAC
                     GAGVITLTIAPDGGIYPCLNLYDGQFEFCNIFDEDFKEKYEKSDIRKEFQTLNISQIN
                     EDCAKCNIKFLCGGRCRGETFQVTNDVKAKYPYCSEWKKAMEKIFWILVEYPELGENK
                     FSQINKNTSEYLNLWH"
     gene            7898..9082
                     /locus_tag="DHV81_03445"
     CDS             7898..9082
                     /locus_tag="DHV81_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI93563.1"
                     /translation="MSIYKNLTMLSTAKFLSGFAGYVYDVGIVIYLYNMTESVAVIGG
                     FFVSQFLPAFVMLMLGEMIDRYNKKRLMIISGFIKAVICLVLLNNRSVWSIYLTTFLM
                     NLILEFEHSTSSALMVDVFSKEEIFKMASVMNVVDSASLIMGPICASFLAAKFDIGIN
                     LVINILLYIISAVIYGFIQMNCMQDIKCTEEKDIKIYRKKGIWRTYIKIFQKERILST
                     VIFWNIFMLCIGIALPLEITMIEDTLKMSSAWYGIGNMVEGLGMMVASAFILGKIKKL
                     KPENIISLGLFTAALSYLMIGISPNIWMYFIGAGLVGMTSTFCPLGFKTEIQVESSPE
                     IVGRTFTTARFTILISRTVGSLIVGGLLNIFSIRIVYYGLTGILMVSAIIFGVRLRDS
                     AT"
     gene            complement(9309..9908)
                     /locus_tag="DHV81_03450"
     CDS             complement(9309..9908)
                     /locus_tag="DHV81_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HCI93564.1"
                     /translation="MSDIIYTYKNEVYANITNKCDCACTFCIRSQKDAIGDATTLWHK
                     VDPTYDEIISAIDAFDFTGYEEFVFCGYGEPTCALEMLEKTAKYVKEKYKLPIRVNTN
                     GLGNLYHGRDIVPELAEYIDSVSISLNAANAEEYNKVTRPSFDNAYEAMLDFAEECNR
                     MIKHTQLSIVDVLPEEDIKTCQQIADERGIHLKIRHFSN"
     gene            complement(10043..10891)
                     /locus_tag="DHV81_03455"
     CDS             complement(10043..10891)
                     /locus_tag="DHV81_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HCI93565.1"
                     /translation="MKKAAVFNDLSGFGKCSLTAAIPVLSAQGVQCCPMASAVLTNQT
                     GYEYHKCTDLTAMIKDYIDNWQKNNAHFDGIYSGFMTGSKQIELFMDFLDVFYEENTM
                     LLVDPVMGDDGRTYGIYSAALLDGMKALSRKADVITPNLTEACLLADYDIEKVYSDTR
                     KDVLLPLATEISEKLRCLSGKNQDVIITGIKCRDDKSPFIYNLVTTANGTTTHRSHFF
                     DVSFSGTGDLFASVICGSRLNGFSTEEAAERAGKFLYDSIADTMNDDTAPNDGVNFEK
                     FLRELM"
     gene            11069..11359
                     /locus_tag="DHV81_03460"
     CDS             11069..11359
                     /locus_tag="DHV81_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD-like/TRCF domain protein"
                     /protein_id="HCI93566.1"
                     /translation="MFQKKQIIYSETLGVCVVDNIVSLAASKREKAVPYYVLKPVFED
                     KVSYIPVEHHRVVLRDMFTREEALKLKETEQYKNDKHLRQAVDYVLDKVAIK"
     gene            11386..11670
                     /locus_tag="DHV81_03465"
     CDS             11386..11670
                     /locus_tag="DHV81_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93567.1"
                     /translation="MGQQHAIHKFVLGTKDFDDKQSEFMYDKGWYSITDIVGEEKNII
                     YKSRNAQEAYLKWNIYIGRKKERLTPEERKRQREERYEKKREQNREHHRI"
     gene            11680..12330
                     /locus_tag="DHV81_03470"
     CDS             11680..12330
                     /locus_tag="DHV81_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCI93568.1"
                     /translation="MGIFNIDNKFFRALNKLVDMVILSFCWIISCIPVFTIGAASTAL
                     YDTSRRVIHHDEGYVWRGYWHAFKVNFKQATKAWLVQLIILIVLLGDIYITWSGLKTG
                     NQWGTFSIVFIIMALIAAAWAIYTSAYISRFEQVTKITLKNTILILIANLPWTLLVIV
                     ILVVSLILAWIIIPLIFILPVGAALTYEFIFERIFRKLMNEEDRLREEEKDKEYRD"
     gene            12375..13478
                     /locus_tag="DHV81_03475"
                     /pseudo
     CDS             12375..13478
                     /locus_tag="DHV81_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080942.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
     assembly_gap    13137..13262
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13513..>14207
                     /locus_tag="DHV81_03480"
     CDS             13513..>14207
                     /locus_tag="DHV81_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCI93569.1"
                     /translation="MSYQALYRKFRPQEFEDVKGQEHIVTTLKNQIKADRIGHAYLFC
                     GTRGTGKTTVAKIFAKAVNCEHPVDGSPCGECPSCRAITEGSSMNVIEIDAASNNGVD
                     NIRQIREEVTYRPTEGRYKVYIIDEVHMLSAGAFNALLKTLEEPPSYVIFILATTEAH
                     KIPITILSRCQRYDFHRISIDTIASRLSDLMEQEQVEVEERAIRYVAKAGDGSMRDAL
                     SLLDQCIAFHLGE"
BASE COUNT         4669 a         2211 c         3095 g         3867 t
ORIGIN      
        1 cgtaactatt ctaacaatat gattttagac catttggaat catgagctta atgtgttatt
       61 acaaataaat attaaaatga aacaacgtat actcttctta atctgttatt tttaacgaga
      121 ttaagaagag tatatttgca ttttgacttg ttttttgcca caaataccgt tatactttac
      181 ttaaagtgta taaaaaacag agattaagaa aagtataacg atataatata ttttgggtgt
      241 aggtgcagga tgcttgtagg tagagaaaaa gaattacatt tattgcatga tatacaaaat
      301 gatgatagct cacattttgt ggcagtatac gggcgaatga gagagggaaa gacctttctg
      361 atacgtgagg cctttggcta tagatttaca tttcagcatg ccggtctgtc agagggagga
      421 atgaaggaac agatatatgc gtttacgtca tctctcaaag atgcagggta tgatatgaaa
      481 aagcagccca aaaactggct tgaggcattt gaagcattaa aggatgtcat aagattgtcg
      541 agtgaaaaga agaaaataat atttattgat gagctgtcgt ggatggacac acaaaagagt
      601 gatcttatgg ttgctttgga gaatttctgg aatggatttg cttctgcaag gaaagatata
      661 gtccttatag tgtgcgcgtc agccacatca tggatgctat caaaggtcgt acacaacaaa
      721 ggaggattgt ataacagatt gacagagcag atacatctgc gcacattctg cctcagggag
      781 tgtgaagcgt atgtaaaagc tgccgggctg gcattaaaca gaaatcagat attgcagtat
      841 tatatgattt ttggcggagt accttactat tggggatttt tgaaaaaggg attaagcctt
      901 tcgcagaata ttgatagtat attgtttgaa aaggatgcgc ctctgcgcga tgaatttaag
      961 tatttatatg cttctgtatt caaaaaaccg gagaattatg tnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaagagc ttgaaagctg tggatttatt
     1141 cgcaagtata atgcatttgg gatgaaaaag aaaaatgcga tataccagtt gatggattgc
     1201 tttacattgt tttatttcaa atttttgaaa tcagaaccga cagatgagca tttttggacg
     1261 aaccagatta atacgcccgc agtgaatacg tggctggggc tggcatttga aagagtgtgt
     1321 atggagcatg tagatcagat taagtttaaa ctgggtattt ctggcgtgct gacagaggta
     1381 aattcgtggt actgcaaatc agatccggat aatggcattt tcggctcaca gattgatatg
     1441 ctgattgcca gaaaagatca ggtgattaat ttgtgcgaga tgaaatattc gcagtcagaa
     1501 tataccataa cggagaaagt ggacaggagc attaggaata aaataagtga tctgagagtg
     1561 gtttccggta caaagtatgc gatataccca acacttatta caacctatgg tgtggtggaa
     1621 aactcgtatt cgcaggagct gcagttggtt gttacgatgg atgatctgtt tgcctgagat
     1681 aaaaaattaa aaaccccata aattcctgtt gacagatagg gattcatggg gtatatttat
     1741 atcaacaatt aattcataca aatacagtat gaatatagga gattaaaaca aagcattaac
     1801 attacaatga catcaaggag gacacaaaca tgtcaaacat ttatcaggga acattaggac
     1861 ttattggaaa tacaccgctc gtagagctta cacacgtaga gaaggcatac ggacttgagg
     1921 caagacttct tgcaaagctt gagtacttca acccggcagg atcagtaaag gacagaatcg
     1981 caaaagagat gatcgagcag gcagagagag atggaaagct caagccgggc tcaacaatta
     2041 tcgagccaac atcaggaaat acaggaatcg gacttgccgc aatcgcagca gcaaagggat
     2101 ataagattat catcgttctt cctgagacaa tgtccatcga gagaagaaac atcatcaagg
     2161 catatggcgc agagctcgtg ctttcagacg gtacaaaggg tatgaaggga gctatcgcca
     2221 aggcagagga gcttcatgag cagattcctg acagcttnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggaaca ggcggaacaa
     2401 tcacaggaac aggtgagtac ttaaagagca agaaccctga tgtaaagatt atcgcagtgg
     2461 agcctgagac atcagcagtg ctttcaacag gaaaagccgg agcacacaag attcagggaa
     2521 tcggagcagg cttcgtacca aacacattaa atacagaggt atatgatgag attatccctg
     2581 tatcaaatga ggcatcattt gagtatggca aggttatagc aaaagaggag ggcgtacttg
     2641 taggtatttc ttcaggagca gctctctatg cagcaatcga ggtggccaag agacctgagt
     2701 tcaagggcaa gacagtagta gcacttcttc ctgacagcgg agacagatac tactcaactg
     2761 atctttttaa ggactaataa agtatattat gatagcgaat aagacacccc tgcggtgtct
     2821 tttttgcata taatttaatt gattctttaa aataccataa actttttcct ttgttttttg
     2881 attctcagct tattatttac atttttgggt ttggagttat gaatatttag ggataatata
     2941 ttagttatat cttgataaaa atgccaaaaa gggataatat attagcatga ttagcttata
     3001 tcaaaaaaca tggacagaaa taaataacga cattgcacaa aagatagtac gtttgcgcaa
     3061 aagaaagaaa atcactcaga agcaattagc tgcaaggtca ggtgtaagtc ttggttcact
     3121 taagcgattt gagcagagcg gtgagatatc cctgcaatct ttgaccaaaa ttgctatagc
     3181 tcttgatgtg gagaacgaac ttgaagactt gtttaataat gtgccttttg cgtctatcga
     3241 ggaggtaatt aatgaacaga ctaaataagc tggaagtctt ttatcatgaa agactcgtcg
     3301 gaacgattgc tttgtaccaa aatcgtctgg ctgcgtttga gtatgacagt aactggctgg
     3361 caaatggatt ttccataagc cctttttcac tgcctttgga gaaaaaagtt tttataccaa
     3421 agatagatcc gtttgagggc ttttagaaac atctcacaga ataccaaatc tcgattatga
     3481 tattctgatg cagcttacac tccagcttac caaatctatg gaagaatgtg aaaaattgta
     3541 tagacttatg tgctttaatg tgtatgcgca taacagggat gaccattcga agaattttac
     3601 ttatctgtat gatgaagatg aatgtagctg gaaattgtca ccggcgtatg acctcaccta
     3661 cagtaattca atcggtggag agcatgctac tacggtaaat ggaaatgctg taaatccgga
     3721 actggatgat attctcgcgg ttgcccaaaa aattggatta aatatgacaa tggcaaggaa
     3781 aaccgcactg aatataagag attgtgttag tgaaatgctt ggggagtatc tatagtgcag
     3841 tgatactatg ttatcccccg aatgtttttt gattaatata ctttttatct aaagcttgca
     3901 cataccatgt aataacaata taatgaaaac cataatatat tatgaaaaaa ggttatgaaa
     3961 aaaagggaaa agatgaagca gtacaaaatt atttaaaaga gcagtaacat agccctttag
     4021 agggttaatc aaactaccag ttgaactggt tgttggtgac ttagaacatt cagaacacta
     4081 tggagcaaaa tggaaagtaa tacactaggc agaatacttc gtgaaaacag ggaaaaacaa
     4141 aagctttcaa agcagaaagt atgccgtgga ctatgtacag taatggcatt gtcaaggtat
     4201 gaatcagatg aacgaatacc ggataaattt ttgatggatg cacttttgga acggcttggg
     4261 tttcagccgt ctaaatatga atttgttgca tctgatcagg aatttgaata tagtatgaag
     4321 cgtgcgcaaa ttgaaaaatt attatatgaa aaaagagatg ttcaggcttg ccgggaagcc
     4381 ttgcaggagt atgaaaaaca ggttcaggaa aaggaacgtc tgcacagaca atatattctt
     4441 cttaaaaagg gacttattct cggacgagaa gaaaaatata gtgatgcaat caatgttttt
     4501 gagcaggctt tgaaatgtac ggaatgcagt gaaactaatg tagcaaatca gacggatatt
     4561 ttattgacta atacagagac agagttgctt tatttgattg ggaaatttct atatatagac
     4621 ggaaagaaga aggatgcctg tacctatttt cgcatgttaa aaaatcaaat ggagcaaaga
     4681 ccagcaggaa aagaaaaatg gaaagaatat tatccttgta tactatatcg cttggcacaa
     4741 tgtgaatttg aagcttataa ttatggcgca agttatgagc ttatcctgca ggctgaaaaa
     4801 gtattgattg aaaattatga atacgattgt ttatatgagg ttttagaatt aaaagaaatc
     4861 atttgtgcaa gacttggaat agctaagaaa acaaatgaaa atttacaact ggcattaaaa
     4921 cttatggcaa tgggagagaa cggaaaattt acaaaagagg gattaaattt atgggagaat
     4981 acagcaagtc agcaattata aaatatacta gaaagtcgct tggaataact caagaggaac
     5041 tttctgagtc ggtatgtgat cctgtaactc tttccagata tgaaagcggg aaaattgatc
     5101 cttcagatag taaatttttg cgccttatgg aaaagatggg ggaaaagggc agtacattca
     5161 tttttcagtt ggaaacaagt cttttagaag tcgaagatga gagtgaaaag ctgaataaag
     5221 cgattgaaca gcatgattgg gaagaagcag agagaataaa aaacaacctt attcaaaata
     5281 ataatttcaa tttggattat tttgaaaacc gacagtatct gcaatgggtg gaaacaatta
     5341 tccaatatga gcagggaatg attaatgaaa gacaggttat tgaaaaactt gaggaggctt
     5401 ggtcatacac atgtggaaat attaatacag aagaatttaa tatgaacaga atttatcgtg
     5461 aaacagagat tattatttta catgaaatcg ctatttttta taagctttta ggaaagctgg
     5521 ataaggcctg cgtcttttat gaaaacttga taaaatattt tgagcgtgct gatatggtaa
     5581 atgatactaa accgatatat ttgatttata taggctattc gaatattctt ggggtaatgg
     5641 gagaacatga gaaaagtatg gaaatttgct ttaaagcaat aaaaagaggg ctggcagaaa
     5701 ataagatgaa ttatctttac aatttctatt ataatattgg atgcagcctg caaataaaag
     5761 gaaatgatga agaaaagcgt ctggcaaagc tgtatatctg gactgcatat cagctttgtc
     5821 aggcttatcc tgaaaataaa agaaaccttg aaataataaa aagaagatat gaacaatttg
     5881 attaataagg attttctaaa tcatataaga gttcaatatc attttcgcat tcatctgatg
     5941 tattattaat atatgatgat gaatttggat ttaaaggttt tgttgttgaa atgatgtaac
     6001 ataaaagtat gccaattaat aaatgttttt ttaatttaga aaatttcata atggttgtcc
     6061 tttcataaaa aaataatgtt ataagtattg ctaaaattat tataaaagac cataacgtat
     6121 tcggttaatc ataattaaac ggatttgcta tggtcttttt gttttagtaa gagtattatt
     6181 tcaaatgaaa gtaatcgttg attataaaaa tataaaataa tagtattcgg aaggaaacat
     6241 ttatgtaatg cagaaagata agttggtact tgaagattac gaaacatttt cagttagagg
     6301 aaaaaataga atagttattt ttccaaatgt tccattttgg attgcggtca cagaaactgg
     6361 atttaaagca ttagaggaaa tgagaaaaaa cggtgataaa gttaaagtgg cacagaaact
     6421 ttatggaaaa tcggaaaaag aacaagtaga ggaaattaac aaattctttt taccactgga
     6481 agaaagtaat gttttacatt cgacattaaa ggggaatgta gtaacaaata gaaaagtatt
     6541 aaaaccaaat aagattacat ttttacaaac aatgagatgc aatttaagat gcaggcattg
     6601 ttgtgtagct gatatgccca atcaaaatct tgaaagcatg tctttggata aagctaagaa
     6661 agcattggac agatgtcttt taatcatgga tgaaaaagaa aagggattat cttttttggg
     6721 tggagaacca ttgtgtggag aaaaatttcc agagttagtg gaatatgcac gatcattagg
     6781 attcaaagtt ggcttatcca caaatggtac tttggtagac gaggcatttg caaggatggc
     6841 aaagaaaaat aatataaatg tgcaaatatc tttggatggc acagatagag agagtcatga
     6901 gtttgtgaga ggcaaaggaa catgggatag tgtaatatcg gcaataagat tattaaataa
     6961 atattgtgta gatatacaaa caaatttagt gtatcacaga ggaaatatta ataaattaga
     7021 agagtacttt gattttgcaa taaacaacaa tattaaaaaa gtaagattga tttcacttat
     7081 gaatatgggg agagctgtgg ggcaaatgga gagggtgcca ttagacgaat ttgtggatat
     7141 aatgtacaag ctaataaaaa aaagacctga tattatagat gtcttggatg aaacttcatt
     7201 tatgggattg gttatgggag cacgtttttc acaaaaaatg gttgcctgtg gagctggagt
     7261 aataactttg acgatagctc cagatggagg catatatcca tgcctaaatt tatacgatgg
     7321 acagtttgag ttttgtaaca tttttgacga agattttaaa gaaaagtatg aaaaatcaga
     7381 tataagaaaa gaatttcaaa cattaaatat ttctcagata aatgaagact gtgcgaaatg
     7441 taatataaag tttctgtgtg gtggacgttg tagaggagag acttttcaag tgacgaatga
     7501 tgtgaaggca aaatatcctt attgcagtga gtggaaaaaa gcaatggaaa aaatattttg
     7561 gattttagta gagtacccag agcttggaga aaataaattt agccaaataa ataaaaatac
     7621 aagtgaatat ttaaatctat ggcattaatt atgtgttgtt actcattaga tggcactatt
     7681 cctgtaattt accaggaaga taaccataaa tatagtttaa ttgagaggag gaatgaaaag
     7741 atgatgttgg caagtgcaca gaccggtcaa aaatcagaaa gctatacgga agtatatggc
     7801 ttaggtaact tcaaagagta ttagttaaat caaagggctg tcaaatggca gcccatttga
     7861 tgtgtgagga atcttatata tgacaaataa agaaaatatg agtatttata aaaatcttac
     7921 aatgttatca actgcaaaat ttctgtcagg ttttgcagga tatgtttacg atgtaggaat
     7981 tgtaatttat ttatacaaca tgacagagtc cgtagctgta atcggaggat tttttgtatc
     8041 acaatttctc ccagcatttg taatgttgat gctgggtgag atgattgacc ggtataacaa
     8101 aaagagattg atgataattt ctggttttat aaaggctgta atttgtttag tattgttaaa
     8161 taatagaagt gtttggagta tttatcttac tacttttttg atgaatttaa ttttagaatt
     8221 tgagcacagt acgtcaagtg cgctaatggt tgatgtgttt tctaaggaag aaatatttaa
     8281 gatggcttcg gtaatgaatg ttgttgattc agcatcacta attatgggac caatttgtgc
     8341 atcatttctg gctgcaaaat tcgatattgg aataaatcta gtaatcaata tattattgta
     8401 tattatatca gcagtaattt atggtttcat acaaatgaat tgtatgcagg acataaagtg
     8461 tacagaggaa aaggatataa agatatatag gaaaaaagga atatggagaa cctatattaa
     8521 gatatttcaa aaagagagaa tattgtctac tgtaattttt tggaatattt ttatgctatg
     8581 cataggaatt gcattaccac ttgaaattac gatgatagaa gataccctta aaatgtcatc
     8641 agcttggtat ggaattggaa atatggttga aggtttagga atgatggtgg cttcagcatt
     8701 tattcttgga aaaataaaga aattaaagcc cgaaaacata atttcgttag gactttttac
     8761 agcagcatta agttatttaa tgataggaat ttcaccaaat atttggatgt attttattgg
     8821 agcaggactt gttggtatga catcaacatt ctgcccgctg ggatttaaga ccgaaataca
     8881 ggtggaaagc agcccggaga tagtaggaag gactttcacg acagcaagat ttacaattct
     8941 tatttcaaga acagtaggct ctcttattgt tggaggactt ttgaatatat ttagtattcg
     9001 aatagtgtac tatggactta cgggaatatt gatggtatct gcaattattt ttggggtaag
     9061 attgagagat agtgcaacat gactaatttc tttacaatgg aagctaagca ggcaaggcta
     9121 tgatagcatc tgatgacgag tgggtcgatg aaactgaatg ggataatatg tttatgaaga
     9181 tgaaaaaaga aagggagaag gatagtgaat aaaggagaag ttcttaactg acaatgaagc
     9241 aatataaata cccattttcc attataacta tattctataa tggaaaatgg gtatttttta
     9301 ctctattcct aattcgaaaa atgacgtatc ttcagatgaa tacccctctc atcagcaatc
     9361 tgctgacagg tcttaatatc ctcctcaggg agtacatcca cgatggaaag ctgtgtatgc
     9421 ttgatcatgc ggttgcattc ctcagcaaaa tccagcatag cctcataagc attgtcaaag
     9481 gatggtcttg tgaccttatt gtattcctcg gcatttgcag cattcaagct tatggaaacc
     9541 gaatcaatat actcagcaag ctccggtacg atatcccttc cgtgatagag attgcctagt
     9601 ccgttagtgt ttacgcggat aggaagctta tacttttcct tcacatattt agcggttttt
     9661 tccagcattt caagagcaca tgttggctca ccatatccac agaaaacaaa ttcctcatat
     9721 cctgtaaaat caaaagcatc aattgcggag attatttcgt cgtatgtcgg atcaactttg
     9781 tgccagagcg ttgttgcatc gcctatagcg tccttctgcg agcgtataca gaatgtacaa
     9841 gcgcagtcac atttattcgt gatatttgca taaacctcgt tcttgtatgt atagattata
     9901 tcagacatta tttagtctcc tccaatgtga aaaatcttct tgtccaaccg ggttatcctt
     9961 tgtgttgtgt taagcctgat ttaagagctc aattgataat ccgataaatt ttctgcaaag
    10021 ggtgataaga attacataaa catcacatca gctcccgcaa aaacttctcg aaattcactc
    10081 cgtcattcgg tgcggtgtca tcattcatgg tatccgcgat gctgtcatac aggaattttc
    10141 cggcgcgctc tgcggcctcc tctgtggaaa atccgttcag cctgcttccg catataacag
    10201 atgcaaacag atcgccggtg ccggaaaagc tcacgtcgaa aaagtgtgat ctgtgggttg
    10261 tagtaccatt tgcggtggtc acgagattgt agataaaggg tgacttatca tccctgcatt
    10321 tgataccggt gatgattaca tcctgatttt tgccggacag gcatcgcagc ttctcgctta
    10381 tctctgtcgc aagaggcagg agcacatcct ttctcgtgtc actgtaaacc ttttcaatat
    10441 catagtctgc caaaaggcat gcctcggtca gatttggtgt gatgacatcg gccttcctgg
    10501 aaagagcctt catgccatcc aaaagagcgg cagagtatat gccgtaggtt ctgccgtcat
    10561 cgcccatcac agggtccacg agcagcatcg tgttttcctc ataaaataca tccaaaaaat
    10621 ccataaaaag ctcaatctgc tttgagcctg tcatgaaacc gctgtaaatg ccgtcaaaat
    10681 gagcattatt tttctgccaa ttgtcgatgt agtccttaat catggctgtc aggtctgtgc
    10741 atttatgata ttcatatcct gtctggtttg tcagcactgc tgatgccatg ggacagcact
    10801 gtacgccctg cgcggacagt accggaattg cggcggtcag agagcatttt ccaaagccgg
    10861 acaaatcgtt aaaaacggct gctttcttca tatatcctcc aaattttttt gcttgatttt
    10921 ctcatacaaa atataggata agtatacatg cgcctgacca catttctatg accagaaaga
    10981 taaaaaaata aatgccagat aaaatgtttt tgaaaaagaa cgctgtctgt gatattatag
    11041 atgtgtatgt aaatacggag gtaaaagcat gtttcaaaag aaacagatta tatatagtga
    11101 aacccttggc gtatgcgtgg ttgacaacat agtatcgctt gcggcatcta agagagaaaa
    11161 ggcggtgcca tactatgtgc tcaagcctgt gtttgaggac aaggtgtcat atataccggt
    11221 agagcatcac cgggtggtac tgcgtgatat gttcaccaga gaagaagctc taaagcttaa
    11281 ggaaactgag cagtataaga atgataagca ccttaggcag gcagttgact atgtactgga
    11341 caaggtggct ataaaataaa attacaacaa ggaggacagg attacatggg acagcagcat
    11401 gcaatacaca aatttgtgct gggaaccaag gacttcgatg ataagcagtc agagttcatg
    11461 tacgataaag gctggtacag catcacagat atagtaggtg aggagaaaaa tatcatctac
    11521 aagtcaagaa atgcccagga ggcatatctt aagtggaata tctatatcgg caggaaaaag
    11581 gaacgcctca cacctgagga gaggaagagg cagcgtgaag aacgctatga gaaaaagaga
    11641 gaacaaaaca gagagcacca caggatatag gagggcatta tgggcatttt caacatagac
    11701 aacaaatttt tcagagcatt aaacaaactg gtagatatgg ttattttgag cttttgctgg
    11761 atcatatcat gcatacctgt ttttacaata ggagcggcat ccacagcact ttacgataca
    11821 tcgcgccgtg tcatccatca cgatgagggc tatgtgtgga gaggctactg gcatgcgttt
    11881 aaggtaaatt tcaagcaggc caccaaggca tggctggtac agcttataat acttatcgtg
    11941 cttttgggag atatttatat tacatggtca ggtcttaaga caggcaatca gtggggaacc
    12001 ttctccatcg tgttcatcat aatggcactt attgccgcag cctgggccat atacacatca
    12061 gcgtatattt cacgatttga gcaggtcaca aagataaccc ttaagaatac aatactcatt
    12121 cttatcgcca acctgccgtg gacattattg gtcatagtga tacttgtggt atcacttata
    12181 cttgcatgga ttattattcc actcattttc atacttcctg tcggcgcagc actcacctat
    12241 gagtttattt ttgagcgtat tttcagaaag ctcatgaatg aggaggatag gctccgcgag
    12301 gaggagaagg ataaggagta cagggattag cataataaaa gacaatcata ataatgcata
    12361 aggaggaagc agcaatgaag aaaattggaa tgcttaccag cggaggagac tgccaggcac
    12421 ttaacgcagc catgagaggc gtggtaaagg cgctggcaca caatgtcgac gagcttgaga
    12481 tttacggctt tgagaacggg tacaagggac tcatatatga gaactacaga atactcacat
    12541 caaaggattt ttcgggcatc ctgacaaagg gcggcacaat actcggctca tcaagacagc
    12601 cttttaagct tatgagagtg ccggatgaga acggactgga caaggtcgca gccatgaaaa
    12661 acacatacaa gaagcttggt cttgactgtc tcgttatcct gggcggaaac ggcacacaaa
    12721 agacagccaa tcttctcagg gaagagggac ttaatatcat tcatctgcca aagacaatcg
    12781 acaatgatat ctacggcaca gatgtgacct ttggcttcca gagcgcgatc aatatagcta
    12841 cagatgcaat cgactgcatc cacaccacag cagcatccca caaccgtgta tttatcgttg
    12901 aggttatggg ccacaaggta ggctggctca cactgtacgc aggtgttgcg ggcggtgcag
    12961 acatcattct gcttcctgag ataccatacg atattgacaa ggtcgtagat gccatccaca
    13021 agagaacaaa ggagggcaag ggctttacca ttcttgcagt agcagaggga gccatctcca
    13081 aggaggatgc agccctttcc aaaaaggaat acaagaagaa gcttgcaaag agcaaannnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnagcccatg cccttacgac agagtgcttt gtacacgtct tggctcagca gcagcaaagg
    13321 ccatcatgga tgaggattac ggctgcatga tagccatggt aaacggacag acaaagaggg
    13381 ttccgcttgc cgacgtggca ggaaagctta agactgtaga tccaaagagc cagatgatta
    13441 aggaagcaaa gcgcaccggt atctgcttcg gagattaagc agcaatatta gatataagga
    13501 aggtgaaaag gcgtgtctta tcaggcccta taccgtaagt tccgtccaca ggagttcgag
    13561 gacgtaaagg gacaggaaca cattgtaaca acactgaaaa atcagataaa agctgacaga
    13621 atagggcatg cctatttatt ttgcggtacg agaggtacgg gaaagaccac tgtggccaag
    13681 atatttgcaa aggcagtcaa ctgtgagcat cctgtagacg gaagcccgtg tggcgaatgc
    13741 ccgtcgtgca gggcaatcac tgagggctca tccatgaatg tcatagagat agatgcagcg
    13801 tcaaacaacg gcgtggacaa tatcagacag attcgtgagg aggtcacata ccgcccgaca
    13861 gagggcaggt acaaggtcta tatcatagac gaggttcaca tgctttcggc aggagccttc
    13921 aatgcacttt taaaaacact tgaggagccg ccgagctacg ttatattcat actggcgacc
    13981 accgaggcac acaagatacc gattactatc ctgtccagat gtcagcgcta tgatttccac
    14041 aggatttcca tcgatacgat tgcatccaga ctcagcgatt tgatggagca ggagcaggtg
    14101 gaggtcgagg agcgcgccat aaggtacgtg gccaaggcag gagacggctc catgcgagat
    14161 gcgttaagtc ttcttgacca gtgcattgct ttccatctgg gtgagaa
//
LOCUS       DPUF01000097            3478 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_56966, whole
            genome shotgun sequence.
ACCESSION   DPUF01000097 DPUF01000000
VERSION     DPUF01000097.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_56966"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="DHV81_03485"
     CDS             complement(<1..212)
                     /locus_tag="DHV81_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCI93570.1"
                     /translation="MANRNIYSVAQVNSYIKNMFAQDFMLRQVSIKGEVSNCKYHTSG
                     HIYFTIKDAGAAMNAIMFAGSRAAGLS"
     gene            complement(245..640)
                     /locus_tag="DHV81_03490"
     CDS             complement(245..640)
                     /locus_tag="DHV81_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N utilization substance protein B"
                     /protein_id="HCI93571.1"
                     /translation="MTRSKIRENVFKMLFRVEFHDKSELAEQMELLNDELTNPTDEEK
                     QYIDEKCSAIIEHMAELDALIDEKSTGWKTNRMAKVDLAIIRLAVYEIKFEDDIPTKV
                     SINEAVELAKKYGADESGAFVNGVLAKFA"
     gene            complement(737..1111)
                     /locus_tag="DHV81_03495"
     CDS             complement(737..1111)
                     /locus_tag="DHV81_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCI93572.1"
                     /translation="MAENRNTINIKSDESGEIKVADEVVAIIAGLAATEVEGVSSMAG
                     NITNEIVSRLGMKNLSKGIQIEIADNEVVVDVALNIAYGYSIPEVSTKVQEKVKSAIE
                     TMTGLSVATINVRIASVDMGEN"
     gene            complement(1134..2282)
                     /gene="glgD"
                     /locus_tag="DHV81_03500"
     CDS             complement(1134..2282)
                     /gene="glgD"
                     /locus_tag="DHV81_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HCI93573.1"
                     /translation="MLNMNNEEALAVIFANSYDALIPEMVSERLMASIPFAGRYRLCD
                     FLISSMVHSGIDNISLIVKKNYHSLMDHLGSGQEFDLARKNGGINIVPPYAQKQIKVY
                     EGRVEAIESLKGYLRKCTQKYVIMADANYVFNFDFRELIEAHKNTGADITAMYRKQEV
                     PKVFLRPNGNNDEMYYTFDIDDGRIKKIYINDRDMGKVNFGMNIYIMEKEKLIRIVDD
                     AFVHGYSYFTRDLMATNTDSLNIQAYEYTGYASQITDMKSYFEENMKLLDEDNRAALF
                     KSGNSIYTKIRDDNPTRYINGSKAKNVMVADGCVIEGTVENSILSRGVKIGKNAKVKN
                     CILLQDTVIEDGANLEYVITDKNVRVSRNRSLTGNDSFQVYVAKGQTV"
     gene            complement(2276..>3478)
                     /locus_tag="DHV81_03505"
     CDS             complement(2276..>3478)
                     /locus_tag="DHV81_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCI93574.1"
                     /translation="ELVAMLLAGGQGSRLYALTQKLAKPAVPFGGKYRIIDFPLSNCV
                     NSGIDTVGILTQYQPFVLNEYIGNGQPWDLDRLYGGVHVLPPYQKASGSDWYKGTANA
                     IYQNIAFIERYDPEYVIILSGDQICKQDYSDFLKFHKEKNAEFSVAVMEVPWEDASRF
                     GLMVADDDDKITEFQEKPKNPKSNLASMGIYIFNWDILKKYLIEDENDPDSENDFGNN
                     IIPNLLRDDRRMYAYHFNGYWKDVGTIPALWEANMEVLDPEHSGINLFDENWKIYSRN
                     SGHTGHFIGNNAEVTDSMITDGCVVKGTVKHSILFGGVIVEEGAVVEDAVVMGDTVIK
                     RGAKVTHCIVAEGVTVGCDAVIGEKPAEDVTGDVATIAPGVTIGDRAVIGPKAMVYND
                     VEEGQEQC"
BASE COUNT          909 a          894 c          576 g         1099 t
ORIGIN      
        1 gaaagtcctg ccgccctgct gcccgcaaac atgatagcgt tcatggctgc tcccgcgtcc
       61 tttattgtga aataaatatg accgctcgtg tgatacttgc agttgctcac ctctcccttt
      121 atgctcacct gtctgagcat aaaatcctga gcgaacatat ttttaatata gctatttact
      181 tgcgctaccg aatatatatt tctatttgcc atgttcaatg ctctatatcg ctgttcctct
      241 taagctatgc aaactttgca agcactccgt ttacaaatgc acctgactca tctgcaccat
      301 acttctttgc aagctcaaca gcctcattta ttgaaacctt tgtaggaata tcgtcctcaa
      361 actttatctc ataaactgcc aatctgataa ttgccagatc aacctttgcc atacgattgg
      421 tcttccagcc tgtagacttc tcatcgatga gtgcatcaag ctctgccata tgctcaataa
      481 tcgcactgca tttttcatca atatactgtt tttcctcgtc tgtaggattt gtaagctcat
      541 catttaaaag ctccatctgc tctgcaagct cagacttgtc atgaaactca accctgaaaa
      601 gcatcttaaa aacgttttct cttatcttgc ttcttgtcat ttaaattcct ctcgtgaata
      661 gtattttata taaattaagg atgtctgtac gacatcctta attatactac atttgtgatc
      721 atttaaaaag aactatttaa ttctctccca tatcaacact tgctatacga acattgatag
      781 tagccacaga aagccctgtc attgtctcaa ttgcagactt aaccttctcc tgtacctttg
      841 tagaaacctc agggatgctg tatccataag caatattgag tgctacatca accactacct
      901 cattgtctgc tatctcaatc tgaattccct ttgagagatt cttcataccg agcctgctga
      961 caatctcgtt tgtgatattg cctgccatgg agcttactcc ctcaacctca gtagcagcaa
     1021 gacctgctat gatagctaca acctcatctg ctaccttgat ctcgccagac tcatctgatt
     1081 tgatatttat tgtgtttcta ttctctgcca tgtccatgtt cctccttaac cggctatacg
     1141 gtctgtccct ttgccacgta aacctggaat gagtcatttc ccgtaagtga tctatttctt
     1201 gatacgcgaa cattcttgtc tgtgatcacg tactccagat tggcgccatc ctcaattacc
     1261 gtatcctgta gcaggatgca gttcttgacc tttgcattct ttcctatctt gacgcctctt
     1321 gataggatgc tgttttccac ggtaccctcg atgacacagc catcagcaac catgacattc
     1381 ttggcttttg agccatttat gtatcttgta gggttatcat ccctgatctt tgtatagata
     1441 gaattgccac tcttaaacaa tgctgccctg ttatcctcat caaggagctt catattctcc
     1501 tcgaaatagc tcttcatatc tgtaatctgt gaagcatagc ctgtgtactc atatgcctgg
     1561 atattgagtg aatcagtgtt tgtagccatc agatcccttg taaaataaga ataaccatgt
     1621 acaaaagcgt catccactat tctaatgagc ttctccttct ccatgatata gatattcatg
     1681 ccaaagttta ccttgcccat atccctgtca ttgatatata tctttttgat acgtccatca
     1741 tctatatcaa atgtatagta catctcatca ttgttgccgt ttggtcttaa gaataccttc
     1801 ggtacctcct gcttgcggta cattgcagtg atatcagccc ctgtgttctt atgtgcctca
     1861 atcagctccc tgaaatcaaa attgaataca tagttggcat cagccatgat aacatacttc
     1921 tgagtgcact ttctcagata gcccttaagg ctttcgattg cctcaacacg gccctcatac
     1981 accttaatct gcttctgtgc ataaggcgga actatattga taccaccatt ttttcttgca
     2041 agatcaaact cctgacctga gcccaggtga tccatcagag agtgatagtt cttcttaact
     2101 atgagagata tattgtcgat tccgctatgt accatgcttg aaatcaggaa atcgcagagt
     2161 ctgtaacgac ctgcaaacgg aatggaagcc ataagtctct cggataccat ctcaggaatg
     2221 agggcatcgt agctgtttgc aaaaataacg gccaaagcct cttcgttgtt catgtttagc
     2281 attgttcctg gccctcctca acatcattat ataccattgc ctttggtcct ataaccgctc
     2341 tgtcgccaat tgtaactccc ggtgcaattg tagcaacatc acctgtcacg tcctccgccg
     2401 gcttttctcc aataactgca tcacagccta ctgtaacccc ctctgcaact atacagtgtg
     2461 tgaccttagc gcctcttttt atcacagtat cacccatgac aactgcatcc tctaccacag
     2521 cgccctcttc tacgatgaca ccgccaaaaa gaattgaatg ctttactgtg cccttcacta
     2581 cacagccatc ggtaatcata gagtctgtaa cctctgcgtt gttgccaatg aaatgtcctg
     2641 tatggccact gttacggctg tatatcttcc agttctcatc aaacaggttg ataccgctgt
     2701 gctcaggatc aagcacctcc atgtttgcct cccagagtgc aggtattgtt cctacatcct
     2761 tccagtagcc gttaaagtga taagcataca tcctacggtc atccctcaaa aggtttggaa
     2821 tgatattgtt accgaaatca ttctctgaat ccggatcatt ttcatcctcg ataagatact
     2881 tcttaagtat atcccaattg aagatataaa tacccatgga tgcaagatta gacttaggat
     2941 tctttggttt ctcctgaaac tctgtgatct tgtcgtcatc atctgctacc atgagaccga
     3001 aacgtgatgc atcctcccat ggaacctcca taaccgctac agaaaactca gcatttttct
     3061 ccttatggaa cttaaggaaa tcactgtaat cctgcttaca aatctgatct cctgaaagta
     3121 tgataacata ctcaggatca tatctctcga taaatgcaat attctgataa atcgcattgg
     3181 ctgttccctt gtaccagtct gagcctgaag ccttctgata cggtggcagc acatgcactc
     3241 ctccatataa tctatcaaga tcccatggct gtccatttcc tatgtactca ttcagcacaa
     3301 acggctggta ctgtgtcaat ataccaactg tatctatacc tgaattaaca caattagaaa
     3361 gcggaaaatc aataattctg tatttgcctc caaacggaac tgccggcttt gcaagtttct
     3421 gtgtcagtgc ataaagtctg gagccctgac caccagcaag aagcatcgca actagctc
//
LOCUS       DPUF01000098            3858 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8673, whole
            genome shotgun sequence.
ACCESSION   DPUF01000098 DPUF01000000
VERSION     DPUF01000098.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8673"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="DHV81_03510"
     CDS             complement(<1..392)
                     /locus_tag="DHV81_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HCI93575.1"
                     /translation="MADNSFNNTVESLFKGMDSFITTKTVVGDAIHIGDTIILPLVDV
                     SFGVAAGAFSAEKKNNGAGGMGGKIQPSAVLVIQNGQTKLVNVKNQDGMTKILDMVPD
                     IVNRFSKGKNDDLNLDDVKVDEAEDDKQ"
     gene            complement(405..1313)
                     /locus_tag="DHV81_03515"
     CDS             complement(405..1313)
                     /locus_tag="DHV81_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93576.1"
                     /translation="MIFTILKVIGIILLVVLALILTALLCVLFVPVKYRAVGSFDNTD
                     IRAKAHVSWLFHLFALHIEYAQETDGYIRLAFVKKKLFDNYEALDDDDMDEAAKTETE
                     DKVSENTGDEPQIPADESAHVADEQTENECTAQTHNEQRTVTDENQHIKQQTKLTHQK
                     TYKKRNKKSHKKQFKDKSEKIKNCIYKLKREYSDERNKAAFLHLKKELFIILKRICPR
                     RLKLTMVYSTGSPDTTGISLGVLACFPVGYTNRWRITPDFESEKPYAKGSFDIKGHVI
                     VISILAATLRILFDKNCRRLYNRISR"
     gene            complement(1318..1638)
                     /locus_tag="DHV81_03520"
     CDS             complement(1318..1638)
                     /locus_tag="DHV81_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93577.1"
                     /translation="MKWYEDLFVGESVTGKIKKIKWKIEHNAGMLHTYIITFPSNEEN
                     LLDIIPTRELLQKGYPKKNLHIIAVAGNYDEALLLACDIVNETYENTGKTDVKSYLKS
                     RRRK"
     gene            complement(1684..2136)
                     /locus_tag="DHV81_03525"
     CDS             complement(1684..2136)
                     /locus_tag="DHV81_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCI93578.1"
                     /translation="MIEATSCGGVVIFRGKILLLYKNYRNRYEGWVLPKGTVESDEEY
                     KDTAIREVKEETGASATIIQYVGKSHYSFTVPHDTVEKDVHWYLMMGESYYSKPQREE
                     YFMDSGFYKYHEAYHLLKFANEKQILEKAYNEYLDLKKSNLWGTHKYY"
     gene            complement(2251..3759)
                     /locus_tag="DHV81_03530"
     CDS             complement(2251..3759)
                     /locus_tag="DHV81_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase"
                     /protein_id="HCI93579.1"
                     /translation="MRQKTDKSRQLKSRGRREVNREGENRQQAAKTARRHRNREYAVV
                     TYFFLIVFVGLIGYFLYFQFVKSDEFINSPYNSLQDLFSKNVVRGEIQTKDGHVIART
                     KVGSDASETREYPDDRMFAHVAGFAVNGKAGLEKQENFSLLRSHEFFLDQIVNDISGK
                     KNTGDNVITTLDYEAQAAAYNALDDYEGAVIAIEPKTGKIAVMVSKPDYDPNTIASDW
                     ESVNGEGSTSLYNRATQGQYAPGSVFKIFTALEYYNENPSTYNDYSFDCDGSITKDGF
                     TIHCAGNKSHGQEDLTKSFAKSCNSSFSNIGLLVDNNKLNTLCDDMLFNKNLPIAFDS
                     KKSKFALDNNASSALTMQTTIGQGNTLVSPLHMCMIAGAICNDGNLMRPYLVDHTENA
                     SGALVEQNKPASYKQIMSKDQAAFLQNLMAAVVSDGIGAKLSDQSYEAFGKTGTAQVS
                     DSTDQTNAWFVGYGKKDGYNDLAIAVIVEDSGAGSKYAVPVAKKVFDKYFNE"
BASE COUNT          960 a          948 c          659 g         1291 t
ORIGIN      
        1 tcctgcttgt cgtcctctgc ctcatctacc tttacatcgt cgagattaag gtcatcattt
       61 ttccccttcg agaagcggtt tacaatgtcc ggcaccatat caagtatctt ggtcataccg
      121 tcctgattct ttacattgac aagctttgtc tgtccattct ggataacgag cactgcactt
      181 ggctgaatct ttccgcccat accgccggct ccgttatttt tcttttctgc cgaaaacgct
      241 cctgctgcca ctccaaacga cacatccaca agcggaagta tgattgtatc ccctatatga
      301 atcgcatcac caactacagt ctttgtagtg ataaagctgt ccatcccctt gaaaagtgac
      361 tctactgtgt tattgaaact attatctgcc ataatatctt cctcctatct gcttatccta
      421 ttatataatc tgcggcagtt tttatcaaat aaaattctaa gtgttgccgc taaaatactt
      481 attactatca catgcccctt tatatcaaag ctgccctttg catacggctt ttcactctcg
      541 aaatcaggtg taatccgcca tctgtttgta tagccgacag gaaaacatgc taaaacacct
      601 aatgaaatgc ctgttgtatc cggcgagccg gttgaataaa ccattgtaag cttaagcctc
      661 cttgggcata ttcttttaag aatgataaaa agctcttttt tcagatgtaa aaaagcggct
      721 ttatttctct catcactgta ttctcttttt agcttataga tacagttttt tattttttct
      781 gacttatctt taaattgctt tttatgtgac tttttgtttc gctttttata cgttttttga
      841 tgagtgagct tagtttgttg ttttatatgc tgattctcat cagttactgt cctttgctcg
      901 ttatgagtct gtgctgtgca ttcattctct gtctgttcat ctgcgacatg tgctgactca
      961 tctgccggta tttgaggctc atctccggtg ttttcactga ctttatcttc tgtttcagtt
     1021 ttagctgcct catccatatc gtcatcatcc aaagcctcat aattatcaaa cagcttcttc
     1081 ttcacaaaag caagccttat atatccgtca gtctcctgtg catactctat atgcagtgca
     1141 aacaaatgaa aaagccacga cacatgtgct tttgctctta tgtctgtatt gtcaaagctt
     1201 ccaacagccc tgtacttaac cggcacaaac agtacgcaaa gcagcgctgt aagtataaga
     1261 gcaagcacta caaggagtat gatgcctatg acctttaaaa tcgtaaatat catctatcta
     1321 tttcctccgt ctgctcttta aatagctctt cacatctgtt ttacctgtat tttcgtatgt
     1381 ctcattaaca atatcacagg ccagcaaaag tgcctcatca taattacccg ctactgcgat
     1441 tatgtgcaga ttcttcttcg gatatccctt ttgcagcaat tctcttgtcg gaatgatatc
     1501 caaaagattt tcctcatttg acggaaacgt gatgatgtat gtgtgaagca tgccggcatt
     1561 atgctctatc ttccacttga ttttctttat ctttccggta acagactctc ctacaaataa
     1621 atcctcatac catttcattc caaaacctca aaaagcagtt ttttaaactg ctttttaata
     1681 atactaataa tacttgtgtg tcccccataa gttgcttttc tttaaatcca ggtactcatt
     1741 gtaagccttc tcaagtatct gcttctcatt tgcaaacttg agcagatgat atgcctcgtg
     1801 atacttgtaa aaccctgaat ccatgaaata ctcttctctt tgcggtttac tataataact
     1861 ttcgcccatc ataagatacc agtgtacatc cttttccact gtatcatgtg gcacggtaaa
     1921 agaatagtgg ctcttaccca cgtattggat tatcgttgca ctggcaccag tctcctcttt
     1981 cacttcacgg attgcagtgt ccttgtactc ttcatcagat tccacagttc ccttgggcag
     2041 tacccacccc tcgtacctgt tcctgtagtt cttgtataag agtaatattt tgccacgaaa
     2101 gattaccaca ccgccacagc tcgttgcttc tatcattgcc attcctccaa gtgtggttta
     2161 aacgctatct gtcagttcaa gcgaaatatg aacgataacg tctgtcatta cacttaagta
     2221 taacctactt ctaaaaaaag tgcaacaagc ctattcattg aaatatttat caaatacctt
     2281 cttggctacc ggcaccgcat atttgcttcc ggcacccgaa tcctccacaa ttactgcgat
     2341 tgccagatca ttgtagccat ccttttttcc atagcccaca aaccaggcat tggtctggtc
     2401 tgtagaatct gatacctggg cagttccggt ctttccaaag gcctcatagc tctgatcgct
     2461 tagctttgcg cctattccat cagataccac tgctgccata agattctgaa gaaaagctgc
     2521 ctgatccttt gacataatct gcttgtatga agccggttta ttctgctcca caagcgcccc
     2581 gctcgcattt tccgtatggt ctaccagata tggcctcatc agatttccat cattgcagat
     2641 tgcaccggca atcatgcaca tgtgaagcgg tgaaacaagc gtgtttccct gacctatcgt
     2701 ggtctgcatg gtcagagctg acgaagcatt gttatccagt gcaaacttac tcttttttga
     2761 atcaaaggct ataggcagat tcttgttgaa aagcatatcg tcacacaagg tattaagctt
     2821 attgttatcc accaaaagac ctatattgga aaaggacgag ttgcatgact ttgcaaacga
     2881 ctttgttaag tcctcctgtc cgtgtgattt attgccggcg cagtggattg taaagccgtc
     2941 ctttgtaata ctgccgtcac aatcaaagct gtagtcgttg tatgtagacg gattttcgtt
     3001 ataatactca agtgctgtaa aaatcttaaa cactgagcct ggagcatact gtccctgtgt
     3061 tgccctgttg taaagagaag tgctgccctc accgtttacg ctctcccagt cacttgcgat
     3121 ggtatttggg tcataatcag gctttgacac cataactgca atctttcctg tcttcggctc
     3181 aattgcaatg acagcacctt catagtcgtc aagtgcatta tatgcagccg cctgtgcttc
     3241 gtaatccagt gtggttatta cattatcacc ggtatttttc ttaccgctta tatcatttac
     3301 tatctggtca aggaaaaact catgtgagcg aagcaatgag aaattttcct gcttttcaag
     3361 cccggcctta ccgtttacgg caaagcctgc cacatgcgca aacatcctgt cgtcagggta
     3421 ttcccttgtc tcagacgcat ctgagcctac ctttgtccgt gctatgacat gtccatcctt
     3481 agtctgtatc tcgcccctca ctacattctt agagaaaaga tcctgcagcg aattgtatgg
     3541 actgttgatg aattcgtcgc tttttacaaa ctggaaatat aagaaataac ctatcaatcc
     3601 aacaaaaact ataaggaaaa aatatgtaac aaccgcatac tctctatttc tgtgtcttcg
     3661 tgctgttttt gccgcctgct gccggttttc tccttctctg ttgacttccc tgcgcccccg
     3721 gctctttagc tgtctgcttt tgtctgtttt ttgtctcatg gtcctttcgt ctcttgtcat
     3781 ctatatttct ccttttttca ggggctgcct gcataatata catgccctgt atcattccaa
     3841 aaataatcat cgtagata
//
LOCUS       DPUF01000099            6115 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4665, whole
            genome shotgun sequence.
ACCESSION   DPUF01000099 DPUF01000000
VERSION     DPUF01000099.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4665"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="DHV81_03535"
     CDS             complement(<1..320)
                     /locus_tag="DHV81_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheY-P-specific phosphatase CheC"
                     /protein_id="HCI93580.1"
                     /translation="MSEFSLDNVNSMYMDVLKEIGNIGAGNATTAIAGMLGTTMKMEV
                     PRVLLMDATKLGDAICPEEETIVGIFLEVTNDISGSMMFLMKLPAAHFLVNKLMGRPQ
                     DYNEP"
     gene            complement(391..861)
                     /locus_tag="DHV81_03540"
     CDS             complement(391..861)
                     /locus_tag="DHV81_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCI93581.1"
                     /translation="MPENEFDIQEKETKQYISVKIGEEKFGIDIGYVDNIVRMQKITR
                     VPKAQKYFKGIINLRGDIVPVMSARIKMGLDDDVYTSSTRIIILKLEEKGEIGLIVDE
                     VCEVVTLVVDEIDKPNIDASSTGKELFINGIGKNGDELISLFEIGSIVSDKDAN"
     gene            complement(876..2996)
                     /locus_tag="DHV81_03545"
     CDS             complement(876..2996)
                     /locus_tag="DHV81_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCI93582.1"
                     /translation="MDVSQYLDIFIDESSEHIQTLSDCIMTLEQEPENKDTINEIFRA
                     AHSLKGMAGTMGFKRMQHLTHDMENVFQEVRSDKIKVDSSMIDLLFKCLDAIDSYVEN
                     IKSTSDEGTDDNEVIIKELNDFIAKANGQTVSKQAEPQEKAAEAETKDSSDASDSALD
                     TIELTDNEKKLIDEAISAGLKIYGMTVTVASDCLLKAARAFLVFKAVEELGQILVYRP
                     SSQDIEDEKFDLSFSFYVASGEGFDKLEHAASNVSEIDKVEGKEITEFHLEGEAEQTA
                     QASEETKKADKEEKEEKTPAAKTESSKPKTANNSQKQAVSHKKATTSRTVRVDIEKLD
                     MLMNQVSELIIAKNSLVAMSSSDGENNNNQSFHEQIEYLERITTNLHESVMKVRMVPI
                     ESVTQKYPRMIRDLSRTLNKKMNLVITGEDTELDRTVVDQIGDPLQHLLRNSADHGLE
                     SNEVRLERGKPEVGTIFLNAYQEGNNVVIKVGDDGNGIDTEAVKNKAIERGIVTAEQA
                     ENLSQKDIINFLFMPSFSMAKQITDISGRGVGLDVVKSGIEQLGGDVSVSTELGKGTT
                     FTVRLPLTLAIIQALMVEIRDEIYAIALGSISNIEDIPVKDIKYVQAKEVIHLRGSVI
                     PIIRLDKMLDIEPKEQEPDHLTVVIVQKGDQQAGLVVDNLIGQQEIVIKSLGKYINGN
                     KLISGATILGDGDVALILDVNTLM"
     gene            complement(2998..4059)
                     /locus_tag="DHV81_03550"
     CDS             complement(2998..4059)
                     /locus_tag="DHV81_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HCI93583.1"
                     /translation="MKSNILIVDDSALLRRVECDIINSDNKLQATDTCKDGLEALEKL
                     RTQKYDAVILDINMPRMNGLEVLSHLQKESIKTPVVVVSTLTIEGADEAIKSLELGAF
                     DFVTKPNNIIEAKGNQFKETLLRVVYAAVRITPAHSASRNLTVVEKKAGAIVNKHKAA
                     GSNKIVAIASSTGGPKALQSVIPFLPAELLAPVVLVQHMPAGFTKSMAERLNELSQVQ
                     VKEASNGEVLKNGVVYIAPGGTHIAIKKSGASHVISFNDMPPISGLKPCANIMFDSLT
                     SSNYDEVVCVVLTGMGADGTNGILSLGKSKPIYVIAQDEKSSVVYGMPRAIYEAGVVD
                     EVVTLSDVAKAITKNVGVK"
     gene            complement(4064..4798)
                     /locus_tag="DHV81_03555"
     CDS             complement(4064..4798)
                     /locus_tag="DHV81_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCI93584.1"
                     /translation="MEYTSLIFPGDKIDIFASGLSDNSTSYKSSFSDKLTDSMWEITM
                     PVDSGRVVLFQLGTQFDFIIYTQNKTILKSSAIVRRRYRKENMYFLAIELINNLEKIQ
                     RRQFFRLPCTIDMYFYEVRYDDKAESEPEFCKKMYKNHAINSKNMNTVKSVILDISGG
                     GIRFSLSTPLEEGTYFMAQFSLALEECTQQFNIVCKVINCTQSLDYADRYFARSKFIF
                     DRMTEREKIVRFVFEEERRIRRREME"
     gene            complement(4819..5697)
                     /locus_tag="DHV81_03560"
     CDS             complement(4819..5697)
                     /locus_tag="DHV81_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HCI93585.1"
                     /translation="MDQAQQLRNVIKQRNQNYIEPARVITITSGKGGVGKSNTSVNLA
                     VWLSRLGKRVIIFDADFGLANVEVMFGVIPKYTLADVIYENQTIKSIISNGPLGIDFI
                     SAGSSVVGLNNLNHKQIHFIVSAINELNSMYDFIIIDTGAGVSEQVMEFVAASNEIVL
                     VTTPEPTSITDSYSLLKALYKRPDFDPSKVCIRVISNRAASKEDGSIVFNKINSVVMQ
                     FLNGSLEYLGYVPSDAMVEKAVRQQKILSIYDPTSKAARSFEEIAKRLLDNESAALDE
                     KRTISHVFSNFFNRKQ"
     gene            complement(5697..>6115)
                     /locus_tag="DHV81_03565"
     CDS             complement(5697..>6115)
                     /locus_tag="DHV81_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HCI93586.1"
                     /translation="SVIYTATELNSAVAEYEQADVIFVDTAGFSHKNETQRNDMRALL
                     AGLSPEYEKSVYLVLSATTKYRDLISIIDSYKDIDDYKLIFTKLDETSSYGNLLNIKL
                     YSDAGISYVTNGQNVPDDIEVFDTQKIVKQLLGGRQ"
BASE COUNT         1656 a         1396 c         1064 g         1999 t
ORIGIN      
        1 ggctcgttat agtcctgtgg tctgcccatc agcttgttta ccagaaagtg tgctgccgga
       61 agcttcatga gaaacatcat actgccggag atatcgttgg taacctcaag aaatataccc
      121 actattgtct cttcctccgg acatatggca tctccaagct ttgtcgcatc cattaacaga
      181 actcttggaa cttccatttt catggtagtg ccaagcatgc cggcaatcgc tgtcgtggca
      241 ttgccggcac ctatatttcc gatttcctta aggacatcca tatacatcga attgacatta
      301 tctaaagaaa attcactcat tgtattcacc cttttatttg ttaaaactta tatagtgagg
      361 cttttatacc tcactataca ttgctgcaaa tcagttagcg tccttatctg agactattga
      421 gcctatttca aacagagaaa tcagttcatc tccgttttta cctataccgt ttatgaacag
      481 ctccttgcct gtagatgaag catctatatt aggcttatct atttcatcaa ccacaagtgt
      541 gacaacttca caaacttcat cgacaataag acctatctca cccttttctt caagctttag
      601 gataattatt ctggttgatg atgtatatac atcatcgtcc agtcccattt ttatgcgggc
      661 actcattacc ggaacgatat caccacgcag atttatgata cccttaaagt acttctgcgc
      721 ctttggcact cttgtaatct tctgcattct gacgatatta tcaacatagc caatatcaat
      781 tccgaatttt tcttcgccta ttttgactga aatatattgc tttgtttctt tttcctgtat
      841 atcaaattca ttttctggca tgtacttccc ctccattaca taagtgtatt tacatcaagt
      901 atcaaagcta catcaccatc tccaagtatt gtcgctccgc tgataagctt gttgccgttt
      961 atgtattttc ctaaagactt gattacaatc tcctgctgtc caatgagatt gtcaaccaca
     1021 agacctgcct gctgatctcc cttttggaca attactacag taagatgatc cggctcctgc
     1081 tcctttggct caatgtccag catcttatcg aggcggatga ttggaataac gcttccacgc
     1141 aagtgaataa cttccttagc ctgtacatat ttgatatcct tcacaggaat atcctctata
     1201 tttgagatag agccgagcgc aatggcatat atttcatcac ggatctctac catgagagcc
     1261 tgaatgattg caagagtaag aggaagacgt acagtaaatg tagtaccctt accaagctcg
     1321 gttgaaaccg atacatcacc acccagctgt tcaatgccgg atttaacaac atcaagacca
     1381 actcctcgtc cggagatatc ggtaatctgt tttgccattg aaaagcttgg cataaaaagg
     1441 aagttgatga tatccttctg agatagattt tcagcctgtt cagctgtgac tatgccacgc
     1501 tcgatagcct tgttctttac ggcttcggta tcgattccgt ttccatcatc tccgaccttt
     1561 atgacaacat tgttgccctc ctggtatgca tttaagaaaa tagtacctac ctctggcttt
     1621 ccacgctcaa gtctgacctc attgctttca agtccatgat cagcactgtt tctaagcaga
     1681 tgctgcagag gatctccgat ctgatccaca actgtacggt ccagctcggt atcttcaccg
     1741 gttatgacaa gattcatctt cttattcaga gtacgagaca gatctcttat cattctagga
     1801 tatttctgtg taacactttc aataggaacc attcgtacct tcatgacaga ctcatggaga
     1861 ttggtcgtaa ttctttccag atattcaatc tgttcatgga atgactggtt attattgttt
     1921 tctccatcag atgagctcat ggctacaaga gagttctttg caataataag ctcagacacc
     1981 tgattcataa gcatatcaag cttttcgata tctactctga ctgttcggct tgtagtagct
     2041 tttttatgag aaacagcctg cttttgagag ttatttgcag tttttggctt tgaagattct
     2101 gtctttgcag caggagtctt ttcctctttt tcttccttat ctgccttttt tgtctcttca
     2161 gaagcctgtg ctgtctgctc tgcttcaccc tccaggtgaa actcggtgat ttcttttccc
     2221 tcaaccttat caatctcaga tacgttggaa gctgcatgct ccaatttatc aaaaccctca
     2281 cctgatgcaa cataaaagct gaatgacaaa tcaaacttct catcctcaat atcctgtgag
     2341 cttggtctgt aaaccagaat ctgtcccagc tcctcaactg ccttgaaaac aaggaaagct
     2401 cttgctgctt tgagcagaca gtcgcttgct acggtaactg tcatgccgta tattttaagg
     2461 cctgctgaaa ttgcctcgtc aattaatttt ttctcgttgt ctgtcagttc aattgtatca
     2521 agtgcactgt cagatgcatc tgatgaatcc tttgtttcag cttcagcagc cttttcttga
     2581 ggctcagcct gtttgcttac agtctgtccg tttgcttttg caataaaatc gttgagttcc
     2641 tttatgataa cctcattatc atcagtaccc tcatcagatg tggatttaat attttcaaca
     2701 tagctgtcta tagcgtcaag gcacttaaag agcagatcaa tcattgagct gtcaacttta
     2761 attttatcgc ttctgacctc ctgaaataca ttttccatat catgtgtaag gtgctgcatg
     2821 cgcttaaagc ccattgtacc tgccataccc tttaaggagt gggcagcacg gaaaatctca
     2881 tttatagtgt ctttgttttc cggctcctgc tcaagagtca tgatacagtc agacaatgtc
     2941 tgaatatgct ccgaactttc atcaataaaa atatctagat actggctaac atccatttta
     3001 ttttaccccc acattttttg tgattgcttt ggcaacatct gatagtgtaa cgacttcatc
     3061 aaccacacct gcctcataga tggctcttgg catgccatac acaacacttg atttctcgtc
     3121 ctgtgcaatg acatagatag gctttgattt gccaagtgaa agaatgccat tcgtgccgtc
     3181 tgcccccata cctgtgagta ccacacatac aacctcatca taattagagg atgtcagtga
     3241 atcaaacatg atattggcac aaggcttgag tccgctgata ggtggcatat cattaaatga
     3301 tataacatgt gaagcgcctg atttctttat agctatatgt gtaccgcccg gagctatata
     3361 cactactccg tttttaagca cctcaccgtt tgaagcctcc ttcacctgaa cctgacttag
     3421 ctcattaagg cgctcagcca tgctctttgt aaagccggca ggcatatgct gtactaaaac
     3481 aaccggtgcc aaaagttcag ccggaagaaa cggtattaca ctttgcagtg cctttggacc
     3541 acctgtagat gatgcaattg ctactatttt attgctgcct gctgccttgt gtttgtttac
     3601 aatagcaccg gctttcttct caacaactgt gagattgcgt gaagcactat gagcaggtgt
     3661 gattctaacg gctgcataca caaccctgag cagagtttcc ttaaactggt ttccctttgc
     3721 ctctatgata ttgtttggtt ttgtgacgaa atcaaaggca ccaagctcta aagatttgat
     3781 agcttcatct gctccctcta tggttagagt gcttaccacc acaacaggtg tttttatgct
     3841 ctccttctgc agatgtgaaa gcacctcaag accattcatc ctcggcatat ttatatcaag
     3901 aataacagca tcgtactttt gtgtacggag cttttcaagt gcttccagtc catccttaca
     3961 tgtatcagtt gcctgtaatt tattgtctga attgattata tcacactcaa cccttctcag
     4021 tagtgcagaa tcatcaacta ttaaaatgtt agatttcata ttattactcc atttctcttc
     4081 gtcttatgcg tctttcttct tcaaacacaa atctgactat tttctcccgt tctgtcatgc
     4141 ggtcgaaaat aaactttgaa cgtgcaaaat atctgtctgc atagtccaaa gactgagtac
     4201 agtttattac cttacaaact atattgaatt gttgtgtaca ctcctcaagt gctaaagaaa
     4261 actgagccat aaaatatgta ccttcttcaa gaggtgtgct tagagaaaat ctgatgcctc
     4321 ctccgcttat atcaaggata actgatttga cagtattcat atttttgctg tttattgcat
     4381 gattcttata catctttttg caaaactcag gctcactctc agctttatca tcgtatctga
     4441 cttcataaaa gtacatgtct atagtacatg gaagtctgaa aaactgacgt ctttgtatct
     4501 tttccagatt atttataagc tctatagcaa gaaaatacat attttcctta cgataacgcc
     4561 gtctgactat agcagagctt tttagtatag ttttgttttg tgtgtatatt ataaagtcaa
     4621 attgcgtgcc aagctgaaat aaaaccactc tgccactgtc aacaggcata gttatttccc
     4681 acatgctgtc tgttagcttg tcagaaaagc tgctcttata tgatgtgctg ttatcggata
     4741 aaccggatgc aaagatatca attttgtcac ccggaaaaat caaacttgta tattccatat
     4801 gtcttcctct taaattaact attgctttct gttaaaaaaa tttgaaaaaa catgtgagat
     4861 tgttcttttc tcatccaaag cagcagattc attatccaaa agtctttttg caatttcttc
     4921 aaatgaccgt gcagcctttg aggtcggatc ataaattgaa agtatcttct gctgcctgac
     4981 agccttttca accattgcat cagacggcac atatcccaga tactcaagac ttccgtttaa
     5041 gaactgcata acaacggaat taatcttgtt gaacactata ctgccatcct cttttgacgc
     5101 agccctgttg gatataacgc gtatgcatac ttttgacgga tcgaaatcag gtctcttata
     5161 cagtgcttta agcaatgaat atgaatcggt gattgaggta ggctccgggg ttgtaaccaa
     5221 cacaatttcg ttgctggccg caacaaattc catgacctgt tcagacactc ctgcaccggt
     5281 atcaattatg ataaaatcat acatactgtt caactcattt attgcagaga caatgaaatg
     5341 aatctgtttg tggtttagat tattaagccc tacaacactt gagccggctg aaataaaatc
     5401 aatgcccaaa ggtccgtttg agatgatgct ctttatagtc tggttttcat atattacatc
     5461 agcaagagta tacttaggaa taacaccaaa catcacttcg acattggcaa gaccgaaatc
     5521 agcatcaaaa ataattactc tctttccgag tctgctgagc cagactgcca gattgacaga
     5581 ggtattggat ttgcctacac cacccttgcc gcttgtaatg gtaataacac gggccggttc
     5641 tatataattt tgattacgct gcttaataac atttctaagc tgctgtgcct ggtccattat
     5701 tgtctgcctc cgagtaattg tttgacaatc ttttgtgtat caaatacctc tatatcatcc
     5761 ggaacgtttt gtccattcgt gacatatgat ataccggcat cagagtatag ctttatattt
     5821 aacagattgc catatgatga ggtttcatca agctttgtaa atatcagctt gtaatcgtca
     5881 atatccttat aggaatcaat tatagatata agatccctgt acttggttgt agcacttaag
     5941 acgagatata ccgacttctc atattcaggt gatagacctg caagaagtgc tctcatatca
     6001 ttccgctgtg tctcgttctt gtgtgaaaag cccgctgtat caacaaaaat cacatctgcc
     6061 tgctcatatt cagccaccgc actatttaac tctgtggcag tataaatgac agaca
//
LOCUS       DPUF01000100            6358 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4499, whole
            genome shotgun sequence.
ACCESSION   DPUF01000100 DPUF01000000
VERSION     DPUF01000100.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4499"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /gene="lon"
                     /locus_tag="DHV81_03570"
     CDS             <1..1883
                     /gene="lon"
                     /locus_tag="DHV81_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCI93587.1"
                     /translation="GKISKELIRQIDQYEDLEKLIDYVTNNLPVSYELKQQVLEAEDI
                     NDRYQVIVSLLLSQVEVISIKNELQKKVKVRVDKHQKEYVLREQLGVIREELGENADS
                     EADEYEKKLSELDAPDYVKEKTKKEIKRFRNMSSSSSESTVERGYIETVLELPWNRMS
                     VDNKDLDHAAQVLDDDHYGLKDVKERILEFLAVRNLTSKGESPIICLVGPPGTGKTSI
                     ARSIASALEKKYVRISLGGVRDEAEIRGHRKTYIGAMPGRIVNGLRQAGVSNPLMLLD
                     EIDKVSSDYKGDTSAALLEVLDSEQNCRFRDHYIEMPVDLSEVLFIATANEVSGIPKP
                     LLDRMELIEVSSYTENEKFHIAKEHLVEKQKSKNGIKKEQLTITDSALKDIIRLYTRE
                     AGVRSLERTIGKLCRKAAREIFKDSEAAVKVTKTNLKTYLGNPKYSPEKKNDRAEVGI
                     VRGLAWTSVGGVTLEVEVNVLPGKGELVLTGKLGDVMKESAQAALSYVRSISEGYGID
                     AEFYTKHDIHIHIPEGAVPKDGPSAGITMATAMLSAITDRAVRADVAMTGEITLRGRV
                     LPIGGLKEKLLAAKVIGIKTVCIPKDNEKDLEEISKEITDGMEIVPVERFSQVEKIAF
                     VK"
     gene            1900..2682
                     /locus_tag="DHV81_03575"
     CDS             1900..2682
                     /locus_tag="DHV81_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCI93588.1"
                     /translation="MVIKSVNLETVCGITSTIPDNEYNEVAFAGKSNVGKSSLINALM
                     NRKSLARISSQPGKTQTINFYNVNDAMYLVDLPGYGYANANIEIKAKWGQMIENYLHT
                     SKKLQAVFLLIDIRHEPSDNDIMMYDWMVNQGFAPIIIATKSDKLKKSQIAAHIKTIK
                     DVLHVKPGTVVIPFSSMSKEGRDEIWNLIDSLVFDEETLLAMKQEEEAKREAKSAVKR
                     AAASQPHKKERWKKTGKPVAKKTKERRDKAKSKKSVNNHKKK"
     gene            2714..>3297
                     /locus_tag="DHV81_03580"
     CDS             2714..>3297
                     /locus_tag="DHV81_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCI93589.1"
                     /translation="MKNKCKFVGIMFLGILIVCTLAVMAYEHTAGMKGAAGGASNASG
                     NTKNTDEADDMLTVVTSFYPMYIATLNIVDGVEGVRLENLSEPQTGCLHDFQLTPEDM
                     KLLSTADVFVINGGGIESFMSDVAKAYPKLDVVEACEDVALLSEDDADSDNDHDHEAD
                     AESDSDHDHDHDADAESDSDHDHDHEADAESDSA"
     assembly_gap    3298..3335
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3336..3851
                     /locus_tag="DHV81_03585"
     CDS             <3336..3851
                     /locus_tag="DHV81_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCI93590.1"
                     /translation="GDENAHAWMSVPRYRTMVQTIASRLAEKDAKHADEYYANAKAYD
                     AKLAVLEEKINSIKSLTNGQNIIIFHEAYAYVADDFSMNACYLLDLDEERSVSAGEIK
                     QVIGAIKDDGVSVILAEELYGKSMGDTVSRETDVHVIYIDPLNRGEYDKDSYLYGMEH
                     NIELIKEAFTK"
     gene            3865..4632
                     /locus_tag="DHV81_03590"
     CDS             3865..4632
                     /locus_tag="DHV81_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCI93591.1"
                     /translation="MRKIIQPCGFHCIKINNLGVSFGEQTVLEDVNMHIHCGSFNVII
                     GQNGAGKSTLIKAILGEIPHTGTIEFKDTKDGHMAKLKIGYVPQSVNIEKNTPVSVYD
                     LIASYQYNYPVFLPKSKKIEAKIRETLEVFEAEELIDKQVCNLSGGQLQRVLLSMAIM
                     DEPNLLLLDEPVSGIDQNGMELFYKTMDYLKTHYDLAIILISHDLDYVAKYADHVVLL
                     DKTVAKQGTVKEVFESKEFERIFYTGEESWVREEEHK"
     gene            4629..5441
                     /locus_tag="DHV81_03595"
     CDS             4629..5441
                     /locus_tag="DHV81_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCI93592.1"
                     /translation="MNITSWLQYDFMRNALIAVLIITPLFGIMGTMIVNKKMAYFSDA
                     LGHSALTGIAVGVVCGMADTSLAMVIFAIVFALLLNKIGSLNTASTDTIISVFSSCSV
                     AIGLAILSKGGNFSKYSSILVGDVLSITKTEIVYLIVIFVVTIVFWVLCFNKLNAICI
                     HRSVAKSKGIHVRLLDNLFAVLVALIVMLSIKWIGILIINALLILPAASSRNISENMR
                     EYHFFSVIFSVFSGIVGLAVSYYTNVATGPMIVIIASVIYFSTFIFGRKLKK"
     gene            5587..6258
                     /locus_tag="DHV81_03600"
     CDS             5587..6258
                     /locus_tag="DHV81_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93593.1"
                     /translation="MLDAISSVVSAVAAQSVTAKDEAQTKNTNTASKKQDAKTDKSDA
                     KTAGFSEEAAVYEKSDSKDDSKDTAKSTVNQKMSKADRDALVQALKDDANARQQQLIE
                     IVRKSMTGQASTWDKSQGLKSLFENLTVDADTIAQAKKDIAEDGYWGVEKTSDRILDF
                     AKALSGGDKDKADELLNAFKKGFSQATGAWGDKLPSICQDTYDAVVKKFDDWKNGTED
                     TQVQG"
BASE COUNT         2015 a         1189 c         1650 g         1466 t
ORIGIN      
        1 tcggcaaaat cagcaaggag cttatcagac agatagatca gtatgaggat cttgaaaagc
       61 tcattgatta tgtaaccaac aatctgccgg tgtcatatga gcttaagcag caggtgcttg
      121 aggcagagga tatcaatgac agatatcagg ttatagtatc attacttctc tcccaggtgg
      181 aggttatttc cataaagaat gagcttcaga aaaaagtaaa ggttcgtgtg gacaagcatc
      241 agaaggagta tgtgcttcgt gaacagcttg gtgtgatacg tgaggagctg ggcgagaatg
      301 ctgactcgga ggctgatgaa tatgagaaaa agctatctga gttagatgcg cctgactacg
      361 taaaggaaaa gacgaaaaaa gagataaagc gctttagaaa tatgagcagc agctcatcag
      421 agagcactgt tgagagaggc tatatagaga ctgtacttga gcttccgtgg aataggatgt
      481 ctgtggacaa caaggatctt gaccatgcag cacaggtgct cgatgatgat cactacggat
      541 taaaggatgt taaggagaga atacttgagt ttctggcagt cagaaatctg acaagcaagg
      601 gagagagtcc tataatttgt cttgtaggtc ctccgggaac aggaaagaca agcatagccc
      661 gttcaattgc gtctgcactg gagaagaaat atgtgcgtat ttctcttggt ggtgtgcgcg
      721 atgaggcaga aatccgtgga cacagaaaga catatatcgg tgctatgccg ggccgtatag
      781 taaatggctt aagacaggca ggcgtttcaa atcctctcat gctgttggat gagatagata
      841 aggtgtcaag tgactacaag ggtgacacat cagccgctct tctcgaggtg cttgacagtg
      901 agcagaactg cagattcagg gatcattaca ttgagatgcc tgtggatttg tcggaggtgc
      961 tttttattgc aacggcaaat gaggtttcag gcattccaaa gccgcttctt gacaggatgg
     1021 agcttattga ggtgtcaagc tacactgaaa atgagaaatt tcacatagca aaggaacacc
     1081 tggttgagaa gcaaaagagc aaaaacggaa ttaaaaagga acagcttacg ataacagact
     1141 cggccctaaa ggatattatc aggctgtaca ccagagaggc cggagtcaga agccttgaga
     1201 ggactatcgg caagctgtgc cgcaaggctg ccagggaaat ctttaaggac agcgaagctg
     1261 ctgtaaaggt tacaaagacc aatcttaaga catatctggg caatccgaag tacagtccgg
     1321 agaagaaaaa tgatcgcgca gaggttggaa tcgttagagg cctggcatgg accagcgtgg
     1381 gcggagtcac actcgaggtt gaggtgaatg tgctaccggg caagggtgag ctcgtgctca
     1441 ccggtaagct tggtgatgtg atgaaggagt cggcacaggc ggcacttagc tatgtgcgct
     1501 caataagcga aggatatggc attgatgcag agttttacac gaagcatgat atccatatac
     1561 atatcccgga gggcgcagtg ccaaaggacg gaccatctgc gggtatcact atggcgacag
     1621 ccatgctctc ggctattaca gacagagctg tcagggcaga tgtagccatg acgggcgaga
     1681 taacactacg cggcagggtg cttccgatag gaggactcaa ggaaaagctg cttgcagcca
     1741 aagtaatcgg aataaaaaca gtatgtattc caaaggataa tgaaaaggac ctggaggaga
     1801 tttcaaaaga aataacagac ggcatggaaa tcgtgccggt ggagagattt tcacaggtgg
     1861 agaagatagc atttgtaaaa taagaatgag gaagagatta tggtaattaa aagtgttaat
     1921 ttagagacag tttgcggaat tacaagcaca atccctgaca atgagtataa tgaggtggca
     1981 tttgccggca agtcaaatgt aggcaagtcg tcactgatca acgcactcat gaacagaaag
     2041 tcacttgcca ggatctcttc acagcccggc aagacacaga ccatcaattt ttataacgtc
     2101 aatgacgcca tgtatctggt cgatctgccg ggctatggct atgccaatgc caatatcgag
     2161 ataaaagcta aatggggaca gatgatagaa aactatcttc acacctcaaa aaagcttcag
     2221 gcagtgtttc ttttaataga tataaggcat gagccatcgg acaatgatat catgatgtat
     2281 gactggatgg taaaccaggg ctttgcaccg attatcattg caaccaaatc tgataaattg
     2341 aaaaaaagcc agattgccgc acacataaag actatcaagg atgtacttca tgtgaagccg
     2401 ggcacggtag ttataccgtt ttcgtccatg tcaaaggaag gcagggacga aatctggaat
     2461 ctcatagatt cactcgtgtt tgacgaggag acgcttcttg ccatgaaaca ggaggaagaa
     2521 gcaaagcgcg aggcaaaatc agctgtaaaa agagccgctg catcccagcc acacaaaaag
     2581 gagcgctgga agaagaccgg aaagccggtt gccaaaaaga ctaaagagcg cagagataag
     2641 gccaagagca aaaaaagcgt taataatcat aaaaagaaat aaagttatag aaagaaaggc
     2701 gaatatttaa tatatgaaga ataaatgcaa atttgtaggc attatgtttt tgggtatatt
     2761 gatagtgtgt acgcttgcgg taatggcata tgagcacaca gcaggtatga aaggcgctgc
     2821 cggtggagca tcaaatgctt cgggcaatac aaaaaatact gacgaagcag acgatatgct
     2881 tactgtcgtg acatccttct atcctatgta cattgccact ttaaacatag tagacggagt
     2941 ggaaggcgtt agacttgaaa atctaagcga gccacagacc ggttgtctgc atgatttcca
     3001 gctcacaccg gaggatatga agcttttatc cacagccgat gtttttgtaa taaacggagg
     3061 cggtatagag tcgtttatgt cagatgtagc aaaggcgtat ccgaagcttg atgtggttga
     3121 ggcctgtgag gatgtggcac ttctttctga ggatgatgca gacagtgaca acgaccatga
     3181 tcacgaagca gatgcagaat cagacagtga tcacgaccat gatcacgatg cagatgcaga
     3241 atcggacagt gaccacgacc atgaccacga agcagacgca gaatcagata gcgcgcannn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggtga tgagaatgcc catgcatgga
     3361 tgagcgtgcc gcgttaccgc accatggttc agacaatcgc atcccggctt gccgaaaagg
     3421 atgcaaagca tgctgatgaa tattatgcaa atgcaaaagc atatgatgca aagcttgcag
     3481 tgcttgaaga aaagataaat agcataaaga gtcttaccaa tggacagaat atcatcatat
     3541 tccatgaggc ctatgcatat gtagctgatg atttttccat gaatgcatgc tatctgttag
     3601 accttgacga ggagcgctca gtcagcgcag gtgagataaa gcaggtaata ggtgccataa
     3661 aggatgacgg agtgtcggta atactcgctg aggagctcta cggaaagagc atgggagata
     3721 ccgtatcgcg tgagacagat gtgcatgtga tttatatcga ccctctcaac aggggagagt
     3781 acgacaagga cagctatctt tatggtatgg agcacaatat agagcttatc aaagaggcat
     3841 ttactaaata aaattggaga aaatatgcgg aaaataatac agccatgtgg ctttcattgc
     3901 ataaaaataa ataaccttgg agtgagcttt ggagagcaga cagtgcttga ggatgtaaat
     3961 atgcacatac attgcggctc tttcaatgtc ataataggcc agaacggtgc cggaaaatct
     4021 acactcataa aggcgatact cggcgagata cctcacaccg gtacgattga gttcaaggat
     4081 actaaggacg gacatatggc taagcttaaa atcggctatg taccgcagtc tgtcaatatc
     4141 gagaaaaaca caccggtcag cgtgtatgac ctaatagcaa gctatcagta caattatcct
     4201 gtttttctgc caaagagcaa aaagatagag gcaaagataa gggagacact cgaggtattt
     4261 gaggctgagg agctcatcga caagcaggtg tgcaacctct cgggaggaca gctgcagaga
     4321 gtgcttcttt ccatggcaat catggacgag ccgaacctgc tactgttgga tgagccggtg
     4381 tccggaatag accaaaacgg catggagctt ttctataaga ccatggatta tttaaagact
     4441 cactatgacc ttgcaatcat actgatatcg cacgatttgg actatgtggc aaagtacgca
     4501 gaccatgttg tgctgcttga taagaccgtt gcaaagcagg gaactgtgaa ggaagtcttc
     4561 gagagcaagg aatttgagag aattttttac acaggagagg aatcctgggt gagagaggag
     4621 gaacacaaat gaatataacc tcctggcttc aatatgattt tatgagaaat gcccttatcg
     4681 ctgtgctcat catcacaccg ctttttggca taatgggaac catgattgta aacaaaaaga
     4741 tggcatactt ttcagatgca ctcggacatt cagcacttac aggaatcgct gtcggagtgg
     4801 tatgcggcat ggcagacacc tcgcttgcca tggtgatatt tgcgatagtg tttgcacttc
     4861 ttttaaataa gataggcagt ctgaacactg catcgacaga taccattata tcggtatttt
     4921 catcgtgctc tgtggcgata ggtcttgcga tactgtcaaa gggcggcaat ttcagcaaat
     4981 actcaagtat actggtgggc gatgtgctaa gcatcacaaa gactgagata gtttatctaa
     5041 ttgtgatttt tgtggtcact atagtgttct gggtgctttg cttcaataag ctcaacgcca
     5101 tatgtataca cagaagcgtg gcaaaaagca agggtatcca tgtgcgtctt ctggataatc
     5161 tgtttgcggt gcttgtagca ctcatagtta tgctatccat caagtggata ggcatactta
     5221 tcataaatgc tctgcttata ttaccggcgg cttcatcaag gaatatttcg gaaaatatgc
     5281 gtgagtatca tttcttttca gtgatatttt cagtattttc cggtatagtc ggcctcgcag
     5341 tgtcatacta caccaatgtg gcaacaggtc cgatgatagt gattattgca tcggtaatat
     5401 atttttcgac atttatattt ggcagaaagc tgaaaaaata acggctaaag ctataagcaa
     5461 cagacgcaaa acagccgata cacaatataa gagaaagact gtttagctat atcagacgct
     5521 atacaggttg ataatagtat agcgggaaag gattcccgca cgcacattac cagggaggga
     5581 tgagaaatgt tagatgctat ttcaagtgta gtttcagcag tagcggcgca gagtgtcaca
     5641 gcaaaggacg aggcgcagac aaagaatacc aataccgctt caaagaagca ggatgcaaag
     5701 acggataagt cggatgcaaa gactgcaggc ttcagcgagg aggcagctgt atatgagaaa
     5761 tcagacagca aggatgattc aaaggatact gcaaagagca cagtaaacca gaagatgagc
     5821 aaggcagaca gagatgcact ggtacaggca cttaaggatg atgccaacgc cagacagcag
     5881 cagcttatcg agatcgtcag aaagtccatg acaggacagg cttcaacctg ggataagtca
     5941 cagggcttaa agagtctgtt tgagaatctg actgtagatg cagacacaat agcgcaggcc
     6001 aaaaaggaca tcgcagagga tggatactgg ggagttgaga agacctcaga ccgtattctt
     6061 gactttgcaa aggcactgtc gggaggcgac aaggacaagg ctgatgagct tttgaatgca
     6121 ttcaagaagg gcttttcaca ggcaacagga gcctggggag acaagctgcc atcaatttgc
     6181 caggatactt atgatgccgt agtcaagaag tttgatgact ggaagaatgg cacagaggac
     6241 acacaggtac agggctagaa gccggatgtg tatgtaaaag tgtcgtgatg gaacgaatat
     6301 tatataagaa atctgattta tgaattatga aaagggggct gcacgcagcg tgcagtca
//
LOCUS       DPUF01000101            7344 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4660, whole
            genome shotgun sequence.
ACCESSION   DPUF01000101 DPUF01000000
VERSION     DPUF01000101.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4660"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            27..137
                     /locus_tag="DHV81_03605"
                     /pseudo
     CDS             27..137
                     /locus_tag="DHV81_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(218..1216)
                     /locus_tag="DHV81_03610"
     CDS             complement(218..1216)
                     /locus_tag="DHV81_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCI93594.1"
                     /translation="MRKIACLSDDDRRELFRNTADKMGLNDAIVEKDFWVCFTLDYLF
                     HRCPWKDSITFKGGTSLSKAFNLISRFSEDIDLILDWRVLGYGILEPWEKRSNTKQDA
                     FNKEANNRAEIFLAEQFCPTIKKELSLELGCDTNIYIDENDKQTVIFAYPNLFTNPST
                     LKVIRLEIGALAAWTPAKLASIEPYTAVYYPKIFEQKNTEILTVSPERTFWEKATILH
                     HEANRPEHLDMPQRYSRHYYDLYRMAQTPVKDVAFSQIDLLKTVVDFKMKFYPRAWAK
                     YPEATPGTLKLIPPEYRFPALNSDYEAMKEMLYGDIPSFNTIMESVRQLEKEINSL"
     gene            complement(1213..1827)
                     /locus_tag="DHV81_03615"
     CDS             complement(1213..1827)
                     /locus_tag="DHV81_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009214062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93595.1"
                     /translation="MSKNYMQEIKSRILASEDGSVFSTSDFADIADTNTVRSALYRLV
                     KDNILRRIINGIYEKPKYSKLLDEYVAADPAAVAQALARSYHWTIAPCGNTALNLLGL
                     STQVAAVWSYISDGPYKTYEWNSTKLEFKHRTNKEITGLSHMTSLVIQALKTLGKKNV
                     TPEVIQTLSTKLSLSEKQTCLKEATESTDWVYDTIRQICGGNTL"
     assembly_gap    2096..2194
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2195..2380
                     /locus_tag="DHV81_03620"
     CDS             <2195..2380
                     /locus_tag="DHV81_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93596.1"
                     /translation="ENIDRMLMERHLNKKARRSSHKNHIKGPKKRTEEDDERFAQSFE
                     KMILMAKSKEELRNTER"
     gene            2458..>4031
                     /locus_tag="DHV81_03625"
     CDS             2458..>4031
                     /locus_tag="DHV81_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93597.1"
                     /translation="MVSTLKLPVGIDSFEKIRRNNFYYIDKTKLIEQLVETGGEVTLF
                     TRPRRFGKTLNMSMLKSFFEVGTDESLFDGLYISGNKELCDKYMGKYPVIFLSLKSAE
                     GRSFDDARYMITELIGREAEKFKFLEHSEALSENDKDRYRAIISLCDGKYTMDEQLLV
                     SSLQSLSQLLFIHYGQKAVILIDEYDVPLDKAFQNGYYKEMVSLIRGLFGKALKTNEF
                     LQFAVLTGCLRVSKESIFTGLNNFEINSIVDIAHDEQFGFTDDEVRKLLTDYDRAERY
                     PDVKEWYDGYHFGNTDIYCPWDVINFAKKLVFDTSARPSAFWINSSGNDMVKRFVDKA
                     DQTTRDEIEKLVAGGFVEKQLRLDLTYDEIDNTIDNLWSVLFTTGYLTTAGEVRLPDS
                     ESYAYKLVIPNKEVREVFVLQIQEWFKAVVAKDDDTMKLLSRAILDKDEKQIARQLNI
                     VMSRMISILDTKASDDMKENFYHGLLLGLLRGSNPGWLIKSNRESGDGFSDILIKPED
                     PDAGIVIEVKYAKEMK"
     assembly_gap    4032..4136
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4200..4823
                     /locus_tag="DHV81_03630"
     CDS             4200..4823
                     /locus_tag="DHV81_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCI93598.1"
                     /translation="MIKNIVFDIGNVLVRFQPDEAMREIGIEENKIAALAHATYENPV
                     WVELDRGVIPENEIIDEMVKVAPQYEEDIRRFFKEGKAFVVKAFDYAADWIKELKSRG
                     YKVYLLSNYPKDYFELHTHSELSFVSLVDGKVISAMVGMIKPDAGIYQCLFDKYNLNP
                     KECVFIDDRPENIEGGRKLGMDGIVFTDYETGKKKLEQMLMAKSKED"
     assembly_gap    4954..5044
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5045..5326
                     /locus_tag="DHV81_03635"
     CDS             <5045..5326
                     /locus_tag="DHV81_03635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCI93599.1"
                     /translation="HQSVLKFASVNLTDNKNVFLICTYGGRPVFKSIEQVIAYKHDNI
                     VGRFSCKGFDTFGPFKLIGGVSKGHPDEKDIAAAVEFYNGLTEQPVFLK"
     gene            5395..6462
                     /locus_tag="DHV81_03640"
     CDS             5395..6462
                     /locus_tag="DHV81_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004057825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HCI93600.1"
                     /translation="MDKLCQNSIELIKYARNITAKQVNNVQIMTYYSLGRWIVEVQQQ
                     GKHRAEYGKKIIINLSKKLTEEFGKGFSESTLEKARKFYLTYKDRISETLFTEFAIKK
                     SETVFTIFEEEHPFVVTWSHYLQLMRIENEDERSFYEIEAAREKWGVRDLSRQYNSSL
                     YERLALSMDKDKVQRLSQEGNVITTSKDVLKDPYVLEFVGLEDKYNYSETDLENRLLD
                     NLQQFLLELGKGFTFVARQKRFSFEEDHFRVDLVFYNRLLKCFVLFDLKIGKLKHQDL
                     GQMQMYVNYYDRYEKTEEENPTIGVLLCNEKNDAMVELTLPEDSNIYASQYKLYLPDK
                     KLLQDKLKEWLQEEGGAEDEI"
     gene            complement(6642..7220)
                     /locus_tag="DHV81_03645"
     CDS             complement(6642..7220)
                     /locus_tag="DHV81_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCI93601.1"
                     /translation="MGTTQPIRNKDELAAFRMYYKDIHPNRRNYCLIVMGLNTALRIS
                     DLLKLKWDNVYNFEHHVFRSHFLINEQKTGKNNYITLNYNATDALRAYFNERHPTEHE
                     FIFTKATCRRQPLNRVQAYRIVRTAAEETVQDEHISCHSLRKTFGYHAWKNGTPPALL
                     MDVYNHSSYKVTQHYLGIEQDERDEIYLNLEL"
BASE COUNT         2217 a         1156 c         1599 g         2077 t
ORIGIN      
        1 gtggaccatt gtaatggggt ggtaatatga agctaaagaa catattaata gtagtggaca
       61 atatcgaaga gtcaatccac ttctataacg aactgtttgg tcttaatgtg atttcgagac
      121 aggtgctaag taatgactgc ttaacaacac gctaaaattt atgcgttgtt gataagctgt
      181 cattaaatag aatgaccagt gccgtttcaa ataattttta aagtgaattg atttctttct
      241 ctaattggcg aactgattcc ataatagtat tgaagcttgg aatatcgcca tataacattt
      301 ctttcatggc ttcataatca ctgttcaggg caggaaagcg gtattccggt ggaatcagct
      361 ttaaggtgcc gggagttgct tctggatact tagcccatgc tcttggataa aatttcattt
      421 taaaatcaac gaccgttttc agaagatcta tttgagaaaa agcaacatct tttactgggg
      481 tctgagccat tctatagaga tcatagtaat gtctggaata tcgttgtggc atatccagat
      541 gctccggtct gttggcttcg tgatgaagaa ttgttgcctt ttcccaaaag gtacgttctg
      601 gtgaaacagt aagaatttca gtatttttct gctcaaagat ttttgggtag tatacagctg
      661 tataaggttc aatgcttgca agcttagcag gggtccaggc tgctaatgct ccgatttcca
      721 ggcgtattac ctttagggtg gaaggatttg tgaacagatt tggataagca aaaataacag
      781 tctgtttgtc attctcatct atgtaaatat tagtgtcaca ccccaattct aatgacagtt
      841 ctttttttat ggtaggacag aactgttctg caagaaaaat ctcagctcgg ttattagctt
      901 ctttattaaa tgcatcttgt tttgtattgg agcgtttttc ccagggctcc agtattccat
      961 aacccagtac gcgccagtca aggatcaaat caatgtcttc tgaaaaacgg ctgatcagat
     1021 taaatgcttt tgaaaggctt gttccaccct taaaagtgat ggagtctttc catggacagc
     1081 ggtgaaataa ataatccagg gtaaagcata cccagaagtc tttttctaca atagcatcat
     1141 ttaagcccat tttatcagcg gtatttcgga aaagctctct tctgtcatca tctgacagac
     1201 atgcaatttt tctcatagtg tattacctcc gcatatttgt cgtattgtat cataaaccca
     1261 gtcggtggat tccgttgctt ctttcagaca ggtctgtttt tcggataaag agagttttgt
     1321 tgaaagagtc tggataactt ccggggttac attttttttc cctaatgttt tcaatgcctg
     1381 aattaccaga cttgtcatat gggacagacc ggtaatttct ttattagttc ggtgtttgaa
     1441 ttcgagcttt gtagaattcc attcataggt tttataaggt ccgtcactga tataggacca
     1501 gaccgccgct acctgagtgg aaagtccaag taaattaagg gcagtgttcc cgcatggagc
     1561 tatggtccaa tgataactgc gggctaatgc ctgtgcaacg gcagcaggat cagcagctac
     1621 atattcatcg agtaatttac tgtattttgg tttttcataa attccattta taattctgcg
     1681 aagaatatta tccttaacaa ggcgatataa tgctgaacga accgtgttgg tatcagcgat
     1741 atccgcaaaa tcagaagtgg aaaaaactga gccatcttcg gaagccagga ttcgactttt
     1801 aatttcctgc atatagtttt ttgacatata aaaacctcct ttgcaacgaa tattatatat
     1861 tattcgttgc atctttgcaa ttacatatgg ttcattattt atatatttgc aagaaataaa
     1921 aaattattta gttgactgaa tacctttgtt accgaaatac attacataaa ctttcaagat
     1981 cgtgttgcag taaattcaat aaatcagtat tatatcttta tgcatacaaa aatcctttaa
     2041 tggagcatta tgagtgtgat ggccagataa acatgttcag gtatctggat aaatannnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaaaat atcgaccgga tgcttatgga
     2221 gagacacctt aacaagaaag ccagacgaag ctcacataag aatcatatca aaggaccgaa
     2281 aaagcgcaca gaggaagatg atgagaggtt tgcacagtcg tttgagaaaa tgatactgat
     2341 ggcaaaatca aaggaagaac taaggaacac agaacggtaa taataattgt attttgcccc
     2401 tcaatccgct atactataac taagtatagc ggattgatac gttttgtgga ggttcatatg
     2461 gtgagtactt taaaactgcc ggtaggaatt gatagttttg aaaaaatcag aaggaacaac
     2521 ttttattata ttgacaagac aaagctcata gagcaattgg ttgaaaccgg tggagaggtc
     2581 acccttttta cacgcccgag gcgttttgga aagactctca atatgagcat gctaaaaagt
     2641 ttttttgagg ttggcacgga tgagtcattg tttgatggct tatatatttc aggcaacaaa
     2701 gagctttgtg acaaatatat gggaaagtac ccggttattt ttctgtcact caagagtgct
     2761 gaaggacgtt catttgatga tgcaagatat atgattactg agctcattgg gagggaagca
     2821 gaaaaattta agtttctgga gcatagtgag gctctttcag aaaacgataa ggacaggtat
     2881 agggctatca tttcgctgtg tgatggtaaa tatacgatgg atgagcagct tcttgtatca
     2941 agtctgcaga gtctgtcgca gttattgttt atacactatg gtcagaaggc tgttatcctc
     3001 atagacgagt acgacgtccc tctggataag gcattccaga atggttacta taaagagatg
     3061 gtttcactta tcagaggact ctttggaaag gcactcaaga ctaatgagtt tctgcagttt
     3121 gccgtactca caggctgtct tcgtgtatcc aaggagagta tttttacagg tctcaacaat
     3181 tttgaaatca attccattgt agatatcgcc catgatgaac agtttggttt tactgatgat
     3241 gaggtcagga agctgcttac ggattatgat agggctgagc gttaccctga tgtaaaggag
     3301 tggtacgatg gctatcattt cggtaatacc gacatttatt gtccgtggga tgtaatcaat
     3361 tttgccaaga agctggtttt tgacacatca gccaggccga gtgcattctg gattaacagc
     3421 agtggcaatg acatggtgaa gcgctttgta gacaaggcag accagaccac gagagatgag
     3481 atagaaaagc tcgtggcagg tggatttgtg gagaaacagc ttcgactgga tctgacttat
     3541 gatgagattg acaatactat agacaatctg tggagcgtac tttttacaac aggatacctg
     3601 actacagctg gtgaggtcag gctgcctgac agtgagagct atgcctacaa gctggtgatt
     3661 ccaaataaag aggtgcgtga agtgtttgtt ctccagattc aggagtggtt caaggctgtt
     3721 gttgcaaaag atgatgatac catgaagctt ttatccagag ctatcctcga taaagatgag
     3781 aagcagattg caagacagct taacatcgtg atgagccgca tgataagtat acttgatacc
     3841 aaggcttctg atgatatgaa agagaacttc tatcatggtc tgctcctcgg cctgcttcgc
     3901 ggaagcaatc cgggctggct tattaagtca aatcgtgagt cgggagacgg tttcagtgat
     3961 attttaatca agccggagga tccggatgca ggcatcgtta ttgaagtgaa atatgcaaaa
     4021 gagatgaaaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatat
     4141 tttagcatat ggcattgctt tttgcaggaa gagatgcagg gttgtaggag aaaaaataaa
     4201 tgattaaaaa catagtattt gacataggaa acgtgcttgt caggttccag ccggacgagg
     4261 caatgcgtga gattggcatt gaagaaaata aaatagcagc tttagcccat gcaacatatg
     4321 aaaaccctgt ctgggttgag ctggaccgtg gtgtaattcc tgagaacgag ataattgatg
     4381 agatggttaa ggtggctcca cagtatgagg aggatatcag gagatttttt aaagagggaa
     4441 aggcttttgt cgtaaaggca ttcgactatg cagcagactg gattaaggag ttaaagagca
     4501 gaggctataa agtgtatttg ttatcgaatt atccaaagga ctacttcgag cttcatacgc
     4561 acagtgaact tagttttgtg tcacttgttg acggcaaagt catttcagct atggttggga
     4621 tgataaagcc tgatgccggt atctatcagt gtctgtttga taaatacaat ctgaatccga
     4681 aagaatgtgt gttcatagat gacagaccgg agaatattga aggtggcaga aagcttggta
     4741 tggatggaat tgtgtttaca gactatgaga ccggaaagaa aaagctggag cagatgctga
     4801 tggcaaaatc aaaggaagac taataaagga ttggagcatt acagatgaag acagcaatag
     4861 tatatgcatc agtgcatcat ggaaacacaa aaaagataat tgacgagata gcaaagacaa
     4921 acgatgtcga gctgatagat gcgacacaga cggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnncatcag tcggtgctta agtttgcatc agtcaatctc acagacaata aaaatgtatt
     5101 tcttatctgt acatatggcg gcagacctgt ctttaagtca attgagcagg tgatagcata
     5161 caaacatgac aacattgttg gaaggttttc atgcaaggga tttgatacgt tcggaccgtt
     5221 caaactgatt ggcggtgtat caaaggggca tccggatgag aaggatattg cggcggctgt
     5281 agagttttac aacggactaa cagaacagcc agtgtttctg aaataggaaa actttgaaat
     5341 aggaggattg aatgtgtgaa gaatgaaatc aatgaatctg tatatgatat caatatggat
     5401 aagttatgtc agaattcaat tgaattaatt aaatatgcac gaaacattac tgcaaagcag
     5461 gtaaataatg tccagataat gacctattat tctcttggca ggtggattgt agaagtacag
     5521 cagcagggaa aacacagggc tgaatacggt aaaaaaatca taataaatct ttctaaaaaa
     5581 ttaacagaag aatttggaaa aggcttttca gaatctacgc tggaaaaggc aagaaagttt
     5641 tatttaacat ataaggatcg aatttcggaa acactgttta cggaatttgc aatcaagaaa
     5701 tcggaaacgg tgtttacgat ttttgaagag gagcatccgt ttgtggtaac atggtctcat
     5761 tatctacaat tgatgagaat agaaaatgag gatgaacgct cattttatga gatagaagct
     5821 gccagagaga agtggggagt cagagatttg tcacgtcagt ataattcttc tctttatgag
     5881 agacttgcgt taagcatgga taaggacaaa gtgcaacgct tatcacagga aggaaatgtg
     5941 attacaacct caaaggatgt gctgaaggat ccatatgttt tggaatttgt aggtcttgaa
     6001 gataaatata attattcaga aactgattta gaaaacaggc tgttagataa tttgcagcag
     6061 tttcttttgg aattgggaaa aggatttact tttgtggcaa gacagaaaag attttcattc
     6121 gaagaagatc atttcagagt ggatcttgtt ttttataaca gactgttgaa atgctttgta
     6181 ttatttgatt taaaaattgg caaattaaag caccaggatc tggggcaaat gcagatgtat
     6241 gtcaattact atgatagata cgaaaaaaca gaggaagaaa atcctaccat tggtgtattg
     6301 ttgtgcaatg aaaaaaatga tgcaatggtt gaactgacat tgccggagga ttctaatatt
     6361 tatgcatcac agtataaact gtatctgccg gataagaaac tattacagga taaattgaaa
     6421 gaatggttac aggaagaagg tggtgctgaa gatgaaattt aattttaaaa tacaacagta
     6481 ccaggcagat gaaagatatg tcacagattg agttgaaatt cgattcagaa cagaatgcat
     6541 ttggtttaac agaggatttc gatgatttgt atatccccaa aaaagccagc cggttgcaaa
     6601 cggctggctt ttagctatat tagatttcaa tacgcataaa actatagctc aagattcaga
     6661 taaatctcat cacgttcatc ctgctcaata cccagataat gctgtgtgac tttataggac
     6721 gagtgattgt agacgtccat cagcagagca ggtggtgtgc cgtttttcca ggcgtgatat
     6781 ccaaatgtct ttctcagaga gtgacagctg atatgttcat cctgaacagt ctcctcggca
     6841 gcagtcctta caattcggta tgcctgaact ctgtttagtg gctgcctccg gcaggtggct
     6901 tttgtaaaaa taaactcatg ttcagtcgga tgccgttcat tgaaatatgc ccggagagca
     6961 tcggtggcat tgtagtttag agtgatatag ttattcttgc cggtcttttg ctcgttgata
     7021 agaaaatgag agcgaaatac atggtgctcg aaattgtata cgttatccca cttcaattta
     7081 agcaggtcac tgattcttag tgcagtgttt aatcccataa caattaaaca gtaattgcgg
     7141 cggtttggat ggatatcttt atagtacatc ctgaaagccg ccagttcatc cttatttcgg
     7201 attggctgtg tagttcccat tgaatactcc tttgcggtag aaaatattat caggcacatg
     7261 ccgcatgaca gactcttccc tggaatatcc aaaatccaca acaaaataat agtaattata
     7321 aaaagcaaaa aatgcaacat ttta
//
LOCUS       DPUF01000102            7848 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7344, whole
            genome shotgun sequence.
ACCESSION   DPUF01000102 DPUF01000000
VERSION     DPUF01000102.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7344"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="DHV81_03650"
     CDS             complement(<1..393)
                     /locus_tag="DHV81_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCI93602.1"
                     /translation="MAEKFEEIAVVVDQSSLGNGIYDLTLKTDKIAKAAKAGQFVSVY
                     SNDKSKLLPRPISLCGINRDDDTIRLVYRVTGEGTGTEEFSRLVRGDKVRILGPLGNG
                     FTVQPGKKAFLIGGGIGVPPMLQLAKDIN"
     gene            complement(446..1378)
                     /gene="pyrF"
                     /locus_tag="DHV81_03655"
     CDS             complement(446..1378)
                     /gene="pyrF"
                     /locus_tag="DHV81_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCI93603.1"
                     /translation="MINKLVDKIKKTNAPIVVGLDPMLSYVPEHITKKAFDELGETPA
                     GAAEAIWQYNKAIVDATYDLIPAVKPQIAMYEQFGVEGLKAFQKTVDYCHEKDLVVIG
                     DVKRGDIGSTSAAYATGHLGKVQIGSKSYSTFDEDFATVNPYLGTDGIKPFVDVCKEE
                     KKGIFILVKTSNPSSGEFQDRMIDGRPLYEWVGEKVAEWGADCMGDSYSYVGAVVGAT
                     YPEQGKILRKVMPKSFILVPGYGAQGGKGADLVHFFNEDGLGAIVNSSRGIICAYKQD
                     KYKDMGITVENFADASRKAVEDMIEDISGALANR"
     gene            complement(1602..2591)
                     /locus_tag="DHV81_03660"
     CDS             complement(1602..2591)
                     /locus_tag="DHV81_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCI93604.1"
                     /translation="MYTADEKRYDTMRYNRCGRSGLKLPVVSLGLWHNFGDFSSYDNM
                     KQMCFTAFDHGITHFDLANNYGPEYGSAEKHFGLILKEHLSAYRDELIISSKAGYDMW
                     KGPYGNWGSRKYLIASLDQSLKRMGLDYVDIFYHHRMDPETPLEETMGALDQIVRSGK
                     ALYVGLSNYDGETMKRACEILTDLKCPFIINQNSYNIFNRTIEQNGLKQAARECQKGI
                     ISFSPLAQGMLTDRYLNGIPEDSRIKTDGRFLNQEKLAPHIEQIKKLNDIATKRGETL
                     AQMALKWVVKDDDVTSVLVGASKPQQILENLKVMEGTGFSEEELRMIDEVVGV"
     gene            complement(2727..3122)
                     /locus_tag="DHV81_03665"
     CDS             complement(2727..3122)
                     /locus_tag="DHV81_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCI93605.1"
                     /translation="MNYTMLKGKIHRAVVKQADLNYVGSITVDTKLLEAAGILEYEMV
                     QIVDVENGNRFETYTIAGEPGSGMICLNGAAARQVAVGDHVILMCYAQMTPEEAKEHH
                     PKVVFVDDENKIARVTNYEKHGKLTEDFI"
     gene            complement(3159..3998)
                     /locus_tag="DHV81_03670"
     CDS             complement(3159..3998)
                     /locus_tag="DHV81_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCI93606.1"
                     /translation="MLIYGQIKQVREAVKEWKREGKSVGFVPTMGYLHEGHKSLIDAA
                     RRENDKVVVSIFVNPMQFGPTEDLDSYPRDLNRDAKLCEDAGVDIIFHPEADEMYADG
                     FCSYVDMNGLTTELCGKTRPIHFRGVQTVVLKLFHIVTPDRAYFGQKDAQQLAVIKRM
                     VKDLNVDTEIIGCPIIREEDGLAKSSRNTYLSADERQAALVLSRSLKIGKQLVDAGEK
                     SAKAVKDAITAEINKEPLAKIDYVDVVDFDTITPVDEIKGTTLVAIAVYIGKTRLIDN
                     FIA"
     gene            complement(4044..4899)
                     /gene="panB"
                     /locus_tag="DHV81_03675"
                     /pseudo
     CDS             complement(4044..4899)
                     /gene="panB"
                     /locus_tag="DHV81_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517323.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
     assembly_gap    4266..4351
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4956..5807)
                     /locus_tag="DHV81_03680"
     CDS             complement(4956..5807)
                     /locus_tag="DHV81_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HCI93607.1"
                     /translation="MKIGIIGAGRVGCSIGKYLRTKDIELAGYYDVDSAAAKEAAEFT
                     RTESFDSLKQLADQSQIIFITTPDSFIIPVWEQLKELSLRNQIICHCSGALSSDSFSG
                     RESMSVSCCSIHPMLPFSNRFSSYEQLNKAFFTVEGQDAAVEAISGLFCSIGNEVCRI
                     DGAKKAKYHAAASILSNQVIAVLDMGYTLLEDCGFTRAEAVRATGQLVMRNIENVLEA
                     GCDGALTGPIERNDIETVKKHIACMQNDDSDDVSLYKMLGTKLANIAEKKNPDKDYTQ
                     MMELLRG"
     gene            complement(5862..6060)
                     /locus_tag="DHV81_03685"
                     /pseudo
     CDS             complement(5862..6060)
                     /locus_tag="DHV81_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743327.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6153..6683)
                     /gene="thiW"
                     /locus_tag="DHV81_03690"
     CDS             complement(6153..6683)
                     /gene="thiW"
                     /locus_tag="DHV81_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCI93608.1"
                     /translation="MESKKNIKKSTISIRKLAMAGVLVAVAVAGSLISFPVAGSKCAP
                     IQHMVNICAAVFLGPVWGVGVAFCASVIRNLTGLGSLMAFPGSMVGALCCGLMYRFSK
                     KIIPTCVAEALGTGILGGLAAYPLAKYVMLIQPEGMFIYIVPFLISTVAGSIVAAIVM
                     LILKKNGALAAFKGNL"
     gene            complement(6799..>7848)
                     /locus_tag="DHV81_03695"
     CDS             complement(6799..>7848)
                     /locus_tag="DHV81_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533846.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiC"
                     /protein_id="HCI93609.1"
                     /translation="MSAVKLGAEAIMDLSSHGNTQPFRQKLTSECPAMIGTVPVYDSV
                     IHYQRDLATLTAQDFVDVVRLHAEDGVDFVTLHCGITRKTIDQIKKHKRKMNIVSRGG
                     SLVFAWMCMTGEENPFYEHYDEILEICEEHDVTISLGDACRPGCLADATDVCQIEELV
                     RLGELTKRAWDHNVQVMVEGPGHVPLDQVAANMKVQQSICMGAPFYVLGPLVTDIAPG
                     YDHITAAIGGAVAAMNGAAFLCYVTPAEHLALPNVEDTKQGIIASKIAAHAADIAKGI
                     PGARDIDDKMADARRVLDWDAQFACALDPETAKAIRDDRLPEDDHSEACSMCGKFCAV
                     RSMNKALAGEYIDIL"
BASE COUNT         1829 a         2057 c         1451 g         2425 t
ORIGIN      
        1 atttatatcc ttggcaagct gcagcatagg cggcactccg attcctccgc cgataagaaa
       61 tgccttcttt cccggctgta ctgtaaagcc gtttccaaga ggtcctaaaa tccttacctt
      121 atcgcccctg acaagccttg aaaattcctc tgttcccgtg ccttcacctg tcacacggta
      181 cacaagacga attgtgtcat catctctgtt tatgccgcaa aggcttatcg gacgcggaag
      241 cagcttactc ttgtcgtttg agtaaaccga tacaaactgt cccgcctttg ctgcctttgc
      301 aattttgtct gtcttaagtg tgaggtcgta gataccattg ccgagtgagc tctggtcaac
      361 tacaactgca atctcctcaa atttctctgc cattttatct ccctggtttc gtttgttaat
      421 atatttaagc tttctgaata acttattaac gatttgcaag tgctcctgag atatcctcaa
      481 tcatatcctc aacagccttg cgtgatgcat ctgcaaaatt ctctactgtg atgcccatat
      541 ccttgtactt atcctgcttg tatgcgcaga tgattccacg tgatgaattg acaattgcac
      601 caagtccgtc ctcattgaag aaatgaacaa ggtctgcgcc cttgcctccc tgtgcgccgt
      661 agcccggtac gaggatgaag ctctttggca ttaccttacg aagaatctta ccctgctcag
      721 ggtatgttgc accgacaacc gcacctacat agctgtatga atcacccatg cagtcagctc
      781 cccactctgc aaccttctcg cctacccact catataatgg gcgtccgtca atcatacggt
      841 cctggaactc tccacttgat ggattagatg tctttacaag aatgaaaata cccttcttct
      901 cctccttgca tacatctacg aatggcttga taccgtctgt tccaaggtat ggattgacag
      961 tggcaaaatc ctcatcaaag gtagaatagc tcttgctgcc gatctgtacc tttccgagat
     1021 gtcccgtagc gtatgcagcc gatgttgagc cgatatctcc acgctttaca tctccgataa
     1081 ccacgagatc cttctcgtga cagtaatcca ctgtcttctg gaatgcctta aggccctcca
     1141 ctccaaactg ctcatacatg gcaatctgtg gctttaccgc aggaatcagg tcatatgtgg
     1201 catccacaat agccttgttg tactgccaga tagcctccgc tgcaccggcc ggtgtctctc
     1261 caagctcatc aaatgccttc tttgtgatgt gctcaggcac atatgaaagc atcgggtcta
     1321 gacctactac gataggtgcg tttgttttct tgattttgtc tactaatttg ttgatcattc
     1381 tttcgtcctc tttatgcgta ttttattata cagacagata ttgtgattat taaatatctg
     1441 catacagtta taaatatttt atagcataaa ctaaattctt gcaacctaaa atataatagc
     1501 agggcgtata gcattaattt ttgctatact ccctgctatt atgcttattg taccttttca
     1561 gattcataca catttacaaa aagcaagtca cgtgcgcaat tctacactcc cacaacctca
     1621 tcaatcatcc taagctcttc ctcagagaag ccagtgccct ccataacctt taagttttca
     1681 agaatctgct gtggctttga ggcgccgaca agcacacttg tcacgtcatc atcctttacc
     1741 acccacttga gtgccatctg tgcaagtgtc tcaccacgct ttgtggctat atcgtttagc
     1801 ttcttaatct gctcgatgtg tggagcgagc ttttcctgat tcaaaaagcg tccgtctgtc
     1861 tttatacggc tatcctccgg aatgccgttt aggtaacggt ccgtgagcat gccctgtgcg
     1921 agcggactga atgagatgat gcccttttgg cactctcttg ctgcctgctt caagccgttt
     1981 tgctctattg tacggttgaa gatattgtat gaattctgat tgatgataaa cggacatttc
     2041 aaatcagtaa gaatctcgca ggctctcttc atggtctcgc catcataatt tgacagtccg
     2101 acatagagtg cttttccgct tcttactatc tggtcgagtg ctcccattgt ctcctcaagc
     2161 ggtgtctcag gatccattct gtgatgatag aagatatcca cgtagtcgag tcccattctc
     2221 ttaagactct gatcaaggct tgcaatcaga tacttgcggc tgccccagtt gccgtaaggt
     2281 cccttccaca tatcatagcc tgcctttgag ctgataatga gctcgtcgcg gtatgccgac
     2341 agatgctcct taaggataag tccgaagtgc ttctctgcgc tgccgtattc agggccatag
     2401 ttgtttgcca ggtcaaagtg ggtgatgccg tggtcaaagg ctgtgaagca catctgcttc
     2461 atgttgtcat acgatgaaaa atcaccgaaa ttgtgccata agccaagcga tacaacagga
     2521 agcttcagtc cgctcctgcc gcatctgtta tatctcattg tgtcatatct cttttcgtct
     2581 gctgtgtaca tatttacctc cctcatgaat acataacatt taaactgttt tacccgataa
     2641 gttattatac tacagcagac ttgatttttc tattttttac taacgaaact tttctatatt
     2701 cctaatgctg tgttactgtt cagaaatcaa ataaaatctt ccgttaattt tccgtgcttc
     2761 tcataatttg tcacacgcgc tatcttgttt tcatcatcca caaacactac cttcggatga
     2821 tgctcctttg cttcctccgg tgtcatctgt gcatagcaca tgagtatcac atggtcaccg
     2881 actgcaacct gtctggctgc tgcgccgttt aagcatatca tgcctgatcc cggctctcct
     2941 gcgatggtgt aggtctcgaa tctgtttccg ttttccacat cgacaatctg gaccatctca
     3001 tactctaata tgcccgctgc ctcaagcagc tttgtatcta cagtgatgct tcccacgtag
     3061 tttaagtcag cctgcttgac cacagctctg tgtattttgc ctttaagcat tgtataattc
     3121 attttttgta cctcttttca ataatcgttt ctgtccgtct acgcgataaa attatcaatc
     3181 agccttgtct ttccgatata tacagcgatt gccacgagtg ttgttccctt aatctcgtca
     3241 accggtgtga ttgtgtcaaa atcaaccaca tcaacataat caattttcgc aagcggctcc
     3301 ttgtttatct ctgctgtgat tgcatccttt accgcttttg cactcttctc gcctgcgtct
     3361 acgagctgct ttccgatttt taagcttctg ctaagtacaa gcgctgcctg tctttcatct
     3421 gcgctcagat atgtgtttct tgagctcttt gccagtccat cctcctcacg gatgattggg
     3481 cagccgatga tttcggtgtc aacattcaaa tcctttacca tacgctttat gactgcaagc
     3541 tgctgtgcat ccttttgtcc aaagtatgcg cggtcaggtg tcacaatgtg aaacagcttc
     3601 aggacaaccg tctgcacacc tctgaaatga atcggtcttg tttttccaca aagctctgtg
     3661 gtgaggccat tcatatccac atatgagcag aagccgtctg catacatctc atcagcctcc
     3721 ggatggaaaa taatgtcaac tcccgcatcc tcacaaagct ttgcatccct gtttaaatca
     3781 cgtggatagc tgtctaaatc ctctgtaggg ccaaactgca ttggatttac aaaaatgctc
     3841 actacaacct tgtcgttttc acgtcttgcc gcatcgatta agctcttgtg tccctcgtgg
     3901 aggtagccca ttgtaggtac aaagcctaca cttttgcctt ctctcttcca ttcctttact
     3961 gcttctctaa cctgtttgat ttgtccgtat attaacattt tatgtctcct tattcttcta
     4021 tgcttcgtaa ctattcttcg atattaatac agcttgtcaa ttattgtctc atccatctta
     4081 aaggtatgct cctcacccgg gaagcttccc tcctcaacag cctttttgta agctgtgaag
     4141 gcctcattca tctcttttcc gacctgtgca aactgcttta caaatttcgg cttcatgttt
     4201 gcatacatgg caagcatatc ctgatatacg agcacctgtc cgtcgcagtc tgctcccgct
     4261 ccgatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncactcgagc accactgcaa atgctcctgc
     4381 ctcctcgact gcgcgggcct cgtcaaggag cttctgtgct gcctcttcac ccttgccctg
     4441 caccttaaag ccgccaaatg cgtttactga ctgtggtgta agccctaaat gtgcacatac
     4501 cggaattgac gcatccacga tagcctttat ctgtggacat acctccctgc caccctcaag
     4561 ctttacagcc tgcgcatggc cttccttcac gagtcgtcct gcattgacca ctgcatcata
     4621 aaccgatgtc tgatatgaca taaaaggcat atctgtaata agcagtgtgt cctttattcc
     4681 tcttgaaaca gccgcgctgt gatgaatcat atcctccata gtcactgaaa gtgtatcctc
     4741 atagccaagc accaccatgc cgagagaatc tcccactaaa atcgcattga ttccggcctc
     4801 gtctatgagc tttgctgtag aataatcata tgctgtgagc atcgtgagct tctcgccctt
     4861 ttcctttgcc tgtttaaaag ttattactgt atttttcatt gtccaatccc tctgtctttc
     4921 tgcaacaatc cgttttttta atattcacgg tattgtcagc ctcttagcaa ttccatcatc
     4981 tgagtataat ccttatccgg attcttcttt tcagcaatat ttgccagttt tgtaccaagc
     5041 attttgtaca agctgacatc gtcactatcg tcattttgca tacatgcgat atgttttttc
     5101 actgtttcaa tatcgtttct ctcgattggc ccggtcagtg cgccgtcaca gcctgcttca
     5161 agtacatttt cgatattgcg catcacaagc tgacctgtgg ctcttaccgc ctcagccctt
     5221 gtaaagccgc agtcctccaa aagagtgtaa cccatgtcaa gaacagcaat cacctgattg
     5281 ctcagaatgc tcgccgctgc atggtatttt gcctttttag caccatcgat gcgacatacc
     5341 tcatttccaa tagaacaaaa cagcccggat attgcttcaa ccgcagcatc ctgcccttcc
     5401 actgtgaaaa atgctttatt taattgctca tatgatgaaa atctgttact gaacggtaac
     5461 attggatgaa ttgaacagca tgatacactc atgctctccc ttccggaaaa tgaatcggat
     5521 gataatgcgc cactgcaatg acatataatc tgattcctta gagacagctc ttttaattgt
     5581 tcccaaaccg gtataataaa tgaatccggt gtggtgataa atataatctg tgattgatcc
     5641 gccagttgct ttagcgaatc aaagctctct gtccttgtaa actccgcagc ctccttagct
     5701 gccgcagaat ccacgtcata atagcctgca agctctatgt cctttgtcct taggtattta
     5761 cctatagaac agcccactct gcctgcgccg attatgccta ttttcatgct accaccttct
     5821 ttccaagccg gtcagaccat ttccttatat cgcagctgat atcatctccg ccatatgccg
     5881 tatggcagcg gtcgatttat cctgtgcgcg cctgtcactt tgctgtctgc ttcatattct
     5941 gatgcagcaa tgtgcttttg gctttcttgt gtctgccgga tatgctctgt cataaggtga
     6001 caacacatcc tccatgatac agtttccttc gaataccgtt cgaagaaaac tataccacat
     6061 tgactattat ttaacaatcc caaattgatt aacttttagt aacaattaag gcttttataa
     6121 tataacgttt ctgtattagt cttaaactat ctctacagat tccccttaaa tgctgccagt
     6181 gcgccatttt tcttaagaat cagcatgaca attgctgcaa ctatgcttcc ggccactgta
     6241 gagataagga atggcactat gtatataaac atgccttccg gctgaatgag catcacatat
     6301 tttgcaagag gatacgctgc aagacctccg agtatgcctg taccaagcgc ctctgcgaca
     6361 catgtcggta tgattttttt gctgaatctg tacatcagtc cacagcacag tgcacctacc
     6421 atgcttcccg ggaatgccat gaggcttcca agccctgtca ggtttctgat gacactcgca
     6481 caaaatgcca caccgacacc ccatactggt cctaaaaata ctgctgcgca tatatttacc
     6541 atgtgctgta tgggtgcaca tttgctgccg gcaacaggaa agcttattaa gcttcctgct
     6601 acggcaactg ccacaagcac tcctgccatg gcaagctttc tgattgaaat agttgatttt
     6661 ttaatgttct ttttactttc cattacttac tgcctcacct taaagtatat cgtaactgtt
     6721 cagtaccttc actgttgctc ctacataata tacgttttct tacgaaatat gcggtaccgc
     6781 attttctgta ctgaatagtt atagtatatc aatatactct cctgcaagtg ccttgttcat
     6841 gcttcgcact gcacagaact taccgcacat actgcatgcc tcgctgtggt catcctccgg
     6901 aagacggtca tccctgatag ccttagctgt ctcaggatca agtgcacacg caaactgtgc
     6961 atcccagtcg agcacacgtc tggcatctgc catcttatca tctatatctc tggctcccgg
     7021 tattcccttt gcgatgtcgg ctgcatgagc tgcaatcttt gaggcgatga tgccctgctt
     7081 tgtatcctcc acatttggaa gagcaagatg ctccgcaggt gtcacgtagc acaggaaagc
     7141 tgctccgttc atcgctgcca ctgctccacc gattgctgct gtgatgtggt catagcccgg
     7201 tgcaatatct gttacgagag gtccaagtac atagaatggt gctcccatac agattgactg
     7261 ctgtaccttc atgtttgctg ctacctgatc aagcggcaca tgtcccggtc cctctaccat
     7321 gacctgtacg ttgtgatccc atgcacgctt tgtgagctct ccaagtctta caagctcctc
     7381 tatctggcat acatctgttg cgtctgctaa acagcccggt ctgcaggcat ctccgagtga
     7441 gattgtcaca tcatgctcct cacagatttc aagtatctca tcatagtgct cgtagaatgg
     7501 attttcctct cctgtcatgc acatccatgc aaagacaagg cttcctccac ggcttacaat
     7561 attcatctta cgcttatgct ttttaatctg gtctatggtt tttcttgtga ttccacagtg
     7621 aagtgtaacg aagtccactc catcctcagc atggagtctg actacatcga caaaatcctg
     7681 tgctgtaagt gtcgcaagat cacgctgata atggattaca ctgtcataaa caggcactgt
     7741 tcctatcatt gcaggacatt cgcttgtgag cttctgtctg aatggctgtg tgttgccatg
     7801 gcttgaaaga tccatgatag cctccgctcc aagctttaca gcactcat
//
LOCUS       DPUF01000103            3106 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10414, whole
            genome shotgun sequence.
ACCESSION   DPUF01000103 DPUF01000000
VERSION     DPUF01000103.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10414"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..>347
                     /locus_tag="DHV81_03700"
     CDS             <1..>347
                     /locus_tag="DHV81_03700"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744095.1"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HCI93610.1"
                     /translation="KHDEYDLIINDSTDPLGHTEGLFTKEFYGSCYKALKDDGIMVYQ
                     HGSPFYDEDELECRKMHRKVYKSFPISRVYQAHIPTCPSGYWLFGFASKKYHPLYDLD
                     AKRWDALGLKTWY"
     assembly_gap    348..432
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            484..1779
                     /locus_tag="DHV81_03705"
     CDS             484..1779
                     /locus_tag="DHV81_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase"
                     /protein_id="HCI93611.1"
                     /translation="MSRLLIIGCGGVAQVAIRKCCQNSEVFTEIMIASRTKSKCEDLK
                     AKLEGKTSTKIETAKVDADNVEELKELINAYKPDAVLNVALPYQDLTIMDACLACGVD
                     YIDTANYECEDTEDPKWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTAILGSG
                     FDPGVTSVYSAYALKHYFDEIHTIDILDCNGGDHGYPFATNFNPEINLREVSANGSYW
                     TDGHWVETKPMEWRAQYNFDQVGEKDMYLLHHEEIESLAKNIPGIKRIRFFMTFGQSY
                     LTHMKCLEDVGMLRTDPIMVDGKEIVPIQVLKELLPDPASLGPRTVGKTNIGCIFTGV
                     KDGKEKSIYIYNVCDHQECYKEVESQAISYTTGVPAMIGAMMVVKGLWKKPGVFNLEE
                     MDPDPYMEALNKFGLPWQVVENPVPVDCYKTEDESVHMD"
     gene            1793..2944
                     /gene="nspC"
                     /locus_tag="DHV81_03710"
     CDS             1793..2944
                     /gene="nspC"
                     /locus_tag="DHV81_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HCI93612.1"
                     /translation="MKINELRTPCYVIDEGKLTENLLILNGVMQRTGARILLAQKAFS
                     AFYEYPLIGRYLSGTTASGLFEARLAHEEMGKENHVFCPAFLPQEMDELVEICDHIVF
                     NSVSQYLLHREKIERADKKISVGLRINPECSTQDGHEIYDPCAPGSRLGITREALDRA
                     IENGLDLSRIEGFHFHTLCEQDSDALETTLAALEEKFGDLLYGLKWLNMGGGHHITRA
                     DYDIERLEKCIMHMRDKYDLEIYLEPGEAVALDAGYLETTVLDIVENNGIKILILDTS
                     AACHMPDVLEMPYRPPLMDSGMPGEKKYTYRLSSCTCLAGDVIGDYSFDKEIKTGDRL
                     RFCDMAIYSMVKNNTFNGMPLPDIAVMDENKDCKVIRRFKYEDFKCRLS"
     gene            2916..>3106
                     /locus_tag="DHV81_03715"
     CDS             2916..>3106
                     /locus_tag="DHV81_03715"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515821.1"
                     /note="catalyzes the formation of carbamoylputrescine from
                     agmatine in the arginine decarboxylase pathway of
                     putrescine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HCI93613.1"
                     /translation="MKILNAGCPRADGFYMPAEYAPHKGTIIIWPKRPGSWIYGAKRA
                     REAFAEVICAIAESEQAYVL"
BASE COUNT          898 a          563 c          813 g          747 t
ORIGIN      
        1 aagcatgatg aatatgatct gataatcaac gattccacag acccgctcgg acatacggag
       61 ggacttttca caaaagagtt ttatggaagc tgctataagg cactaaagga tgatggaatc
      121 atggtttacc agcacggcag ccctttttat gatgaggatg agcttgagtg ccggaaaatg
      181 cacagaaagg tgtataaatc atttcctata agcagggtat atcaggcaca tattccaacc
      241 tgtccgtcag gctactggct gtttgggttt gcatcgaaga aatatcatcc gctttatgat
      301 ctggatgcaa aaagatggga tgcactgggg cttaaaacct ggtattannn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnaaacaaat tagaaaaact atatcataac caaagcgaat caggaggaca
      481 aaaatgagca gattacttat tatcggatgt ggtggagttg cgcaggtggc aatcagaaaa
      541 tgctgccaga acagcgaggt atttaccgag attatgattg caagcagaac caagtcaaaa
      601 tgtgaggatt taaaggcaaa gcttgaggga aagacaagca caaaaattga gactgcaaag
      661 gttgatgccg acaatgtgga ggaattaaaa gagctgatta atgcatacaa gccggatgcg
      721 gttttaaatg ttgcgcttcc gtatcaggat cttacaatta tggatgcatg tcttgcgtgt
      781 ggtgttgact acatcgatac tgccaactat gagtgcgagg atactgagga tccaaagtgg
      841 agagcaatct acgaaaagcg ctgcaaggag cttggattta cggcatattt tgattattca
      901 tggcagtggg catatcagga gaaattcaag gaggcagggc ttacagctat tcttggaagc
      961 ggatttgacc cgggtgtgac ctctgtttat tctgcatatg cgcttaagca ttattttgat
     1021 gaaatacata ctatagacat tctcgactgc aatggcggtg accatggata tccgtttgcg
     1081 acaaacttta atccggagat aaatctgcgt gaggtttctg cgaacggctc atactggaca
     1141 gatggtcact gggtagagac aaagcctatg gagtggagag cgcagtacaa ttttgaccag
     1201 gtcggagaaa aggatatgta tctgcttcac cacgaggaga ttgagtcact ggccaaaaac
     1261 attccgggaa taaagagaat cagattcttc atgacctttg gacagagcta cctcacgcac
     1321 atgaagtgtc ttgaggatgt aggcatgtta aggacagacc cgatcatggt ggacggaaag
     1381 gaaatagtgc caatacaggt gcttaaggag ctgcttcctg accctgcatc cttgggacct
     1441 cgtacagtcg gaaagacaaa tatcggatgt atttttacag gtgtcaagga cggaaaggaa
     1501 aagtcaatct acatctataa tgtatgtgac catcaggagt gctacaagga ggttgaatcg
     1561 caggcaattt cctataccac aggtgtgccg gcaatgattg gagccatgat ggtggtaaag
     1621 ggactttgga agaagccggg agtgttcaac ctcgaggaga tggatccgga tccatacatg
     1681 gaggctctta ataagttcgg actgccttgg caggtagtgg agaatccggt tcctgtggat
     1741 tgctataaga ctgaggatga gtcagtacat atggattagt ccggaggccg gtatgaagat
     1801 aaatgaatta agaactccct gttatgtgat agacgaggga aaattaactg aaaatctttt
     1861 gatattaaac ggtgtcatgc aaaggacagg tgcaaggatt cttctggcac agaaggcatt
     1921 cagcgcattt tatgaatatc ctcttatcgg cagatatcta agcgggacaa ctgcaagcgg
     1981 gctttttgag gcccgtcttg cacatgaaga aatgggaaag gaaaaccatg tattctgtcc
     2041 cgcttttttg ccgcaggaga tggatgagct ggtagaaata tgtgaccata ttgtatttaa
     2101 ttccgtatcg cagtatctgc ttcacaggga gaaaatcgaa agggctgata aaaaaataag
     2161 tgtcgggctc agaataaacc ctgagtgctc tacacaggat gggcatgaga tatatgaccc
     2221 atgtgcgccg ggctcaaggc ttgggataac gagagaggca ctcgataggg ctattgaaaa
     2281 tgggcttgat ttatcacgta tagagggctt tcattttcac acgctctgtg aacaggattc
     2341 ggatgcgctc gaaacgactc ttgcagcgct cgaggagaaa ttcggagatt tgctgtatgg
     2401 acttaagtgg ctcaatatgg gaggaggaca ccacatcaca agggcagact atgacattga
     2461 gaggcttgaa aaatgcatta tgcatatgcg tgataaatac gacctggaga tctatctgga
     2521 gccgggagag gcagttgcgc ttgatgcagg ctatctggag actacagtgc ttgatatagt
     2581 tgaaaacaat ggcataaaga tactcattct ggatacctcc gcagcctgcc atatgccgga
     2641 tgtgcttgag atgccgtacc gtccaccgct tatggacagt ggcatgccgg gtgagaagaa
     2701 atatacatac agactgtcct catgtacctg tctggcaggt gacgtgatag gggattattc
     2761 atttgacaag gagataaaaa ccggtgacag actgcgcttt tgcgatatgg caatctactc
     2821 gatggtcaaa aataatacct ttaacggcat gccgcttccg gacattgccg taatggatga
     2881 aaataaggat tgtaaggtta tcaggagatt caaatatgaa gattttaaat gcaggctgtc
     2941 ctagggcaga cggcttctat atgccggcgg agtatgctcc ccacaagggc accatcatca
     3001 tctggccgaa gcgcccgggc tcatggattt acggtgccaa gcgggcaaga gaggcttttg
     3061 cggaggtgat atgtgcgata gcagagtctg agcaggcata tgtgct
//
LOCUS       DPUF01000104            7705 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_14224, whole
            genome shotgun sequence.
ACCESSION   DPUF01000104 DPUF01000000
VERSION     DPUF01000104.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_14224"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="DHV81_03720"
     CDS             complement(<1..554)
                     /locus_tag="DHV81_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch synthase"
                     /protein_id="HCI93614.1"
                     /translation="MKKILFVASECVPFIKTGGLADVCGALPKEFDKNYWDVRVVIPD
                     YSCIPEQYRNNFEYVTHFYMSSGPYVQDKYVGVLKYEQDGITYYFIDNQEFFTGFSPY
                     TSDTKFEIEKYTFFDKAVLSMLPLIDFKPDIIHCHDWQTGLLPVYLKNEFAANPFFWG
                     IKTIITIHNLKFQGIWDREWVQGVS"
     gene            complement(595..1323)
                     /locus_tag="DHV81_03725"
     CDS             complement(595..1323)
                     /locus_tag="DHV81_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI93615.1"
                     /translation="MSGHSKFANIKHKKEKNDAAKGKIFTMIGRELAVAVKEGGPDPA
                     NNFKLAQVVAKAKANNMPNDTIERGIKKAAGDGNSVNYETATYEGYGPSGTAIIVKCL
                     TDNKNRTAANVRNAFTKGQGSIGTQGCVSYMFDEKGQIIIDKEECDMDADDLMMQALD
                     AGAEDFADEDDSYEITTAPADFDAVRTALEEAGITMASAEVTMIPQTYVTLTDEADIT
                     NIGRILDLLDDDDDVQEVYHNWEE"
     gene            complement(1373..2284)
                     /locus_tag="DHV81_03730"
     CDS             complement(1373..2284)
                     /locus_tag="DHV81_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCI93616.1"
                     /translation="MLYAIIILSILVVILLLCIGVLLYGMKNNKKTLDGILGNDDVTE
                     EEIISMVREGHEQGTILASEAELIHNVFEFDDKEVKDIMTHRKNIVSLDGSMSFIDAI
                     EFIIDTGKSRFPVYENDVDSIIGVLHIKDAFTFFEKNEVYRSSIKDIDGLIRPVDFIP
                     ETVNINDLFKKMQSKKSHLAMVVDEYGQISGLIAMEDILEELVGNIEDEHDEEENYIR
                     KNDDETFIMDGMTEFSDVKEALSLPVDDDAYETLNGFIISLSDKIPEEGDKTVISAYG
                     YRFSVMSVEDKVIKQVMIKKLAPEEKK"
     gene            complement(2299..2646)
                     /locus_tag="DHV81_03735"
     CDS             complement(2299..2646)
                     /locus_tag="DHV81_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCI93617.1"
                     /translation="MIIQRGDIYYADLRPVVGSEQGGIRPVLIIQNDVGNRHSPTVIC
                     AAITSQMHKAKLPTHVELACKEYALAKDSVVLLEQLRTIDKKRLKDKVCHLDEDTLLK
                     IDRALEISLELIT"
     gene            complement(2725..3855)
                     /gene="alr"
                     /locus_tag="DHV81_03740"
     CDS             complement(2725..3855)
                     /gene="alr"
                     /locus_tag="DHV81_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCI93618.1"
                     /translation="MAINRIRADIDLDAILYNMESMHNKLKPGTMMAAVVKADGYGHG
                     AEAVSEVLEPLPYLWGYAVATSNEAMQLIAAGRKKPILILGLSFPEQFEEIVDNDVRP
                     AVCTYETAKELSRIAVAKNKTCKIHIKIDTGMCRIGFQVTEESADIIAQIAKLPNIMI
                     EGIFTHFARADELSKEPAFEQFDLFKRMIALVEARGVEIPIKHCSNSAGIVEIPECNM
                     DMVRAGITLYGLWPSEEVDKSKISLKPVMSLHSRVAYVKELEPGRHISYGGTFTVEHH
                     MRIATVPVGYGDGYSRGLSNKGWVLIKGKKAPICGRVCMDQFMVDVTDIPDVKIGDAV
                     TLLGKDGDETITMEQLGELSGRFNYEFACLITPRVPRIYHKN"
     gene            complement(3845..5347)
                     /locus_tag="DHV81_03745"
     CDS             complement(3845..5347)
                     /locus_tag="DHV81_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCI93619.1"
                     /translation="MEYIVTGNEMKLLDDTTSQVFLVPSVVLMEQAAAGVVRELVACF
                     DKSKEFAVICGRGNNAGDGIAIARLLNQAGYRCMVYYAFGTDEAGSELFELQKNIYAR
                     YGFKVVSDIECVCKSDVIIDALFGTGLKRAIEGSLADVISYVNKTDAYRVAVDVPSGV
                     DSDKGHVMGCTFKADFTYTFSYKKTGLLLWPGCDYCGLVKVVPIGIDDHSFCGNLPSV
                     RALDESDLKKLDNRPAHSNKGTFGKLLVIAGSRNMAGAAVLSAKAAYKSGAGLVKIIT
                     PECNRSIIQCALPEALLCTDIASAKALETELEWADAVVIGPGLSKSDNAKMLVETVLK
                     TAEIPLVIDADALNIISDNMTLLNEHKMPVIVTPHLGEMSRLMKKSIANIQEDIIGVA
                     GEFASLYNVTCVLKDFRTIIAAADGEKFINLSGNCGMATAGSGDVLSGIVGGLLVQWR
                     DTKKAAAYGAFIHGLAGDMVFDNTGSYGMIASDIIDGLDKVWIKVEKNGN"
     gene            complement(5350..5991)
                     /locus_tag="DHV81_03750"
     CDS             complement(5350..5991)
                     /locus_tag="DHV81_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93620.1"
                     /translation="MNDKKDFEAALKGKKVPLLVLDQKWHRLFAIHGKTDEISATEKE
                     LDELLKQQGKYNNELKNLKKLKSKIMSNIVANMGDDGDENRDKDKQLIDEINGKADNI
                     EGELIEIQKNIKAVNDRLMLLSMDYFSEKIEKNKLESKEIDDWIANIRVELKKNVIRK
                     QNRDINNREIYSYLHDIFGAEVLDLFDIEYDDPMVFNANNANTDNANNENKGN"
     gene            complement(6004..6642)
                     /locus_tag="DHV81_03755"
     CDS             complement(6004..6642)
                     /locus_tag="DHV81_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCI93621.1"
                     /translation="MSKEISQAVIRRLPRYYRYLGELLDEGVERISSNDLSHRMKVTA
                     SQIRQDLNNFGGFGQQGYGYNVQFLYEEIGKIMGLNTEHRIIIIGAGNLGQALANYVK
                     FEKLGFVITALFDVNPELSGKSVRGIPILMLSELDEYCRTHRVDIAALTMPKSKADSI
                     ASKLVDLGIRAIWNFAHVDLEIPNKDVVVESVHLSDSLMQLSYNIVKNSKNK"
     gene            complement(6814..>7461)
                     /locus_tag="DHV81_03760"
     CDS             complement(6814..>7461)
                     /locus_tag="DHV81_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCI93622.1"
                     /translation="ESFAHSCFKYALDTKQDLWFATKDTISKKYDHTFKDIFQEIFDA
                     QYKDAFEKAGITYFYTLIDDAVARVMKSEGGFIWACKNYDGDVMSDMISSAFGSLAMM
                     TSVLVSPHGYYEYEAAHGTVQRHYYKHLKGEETSTNSVATIFAWTGALRKRGELDKIP
                     ALSEFADKLEAACIKTIESGKMTKDLALITTIENPTVLNSEGFIKAIREELEKTI"
     assembly_gap    7462..7568
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7569..>7705)
                     /locus_tag="DHV81_03765"
     CDS             complement(<7569..>7705)
                     /locus_tag="DHV81_03765"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569515.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCI93623.1"
                     /translation="IEPNVKTWVKPITIARHAYGDVYKATEMKVAGPGKAELVFTAED
                     GT"
BASE COUNT         1994 a         1910 c         1288 g         2406 t
ORIGIN      
        1 gaaacaccct gtacccactc cctgtcccat attccctgga acttaagatt gtggatagtg
       61 ataattgtct tgatacccca gaagaatgga tttgcggcaa actcattctt taagtataca
      121 ggcagaagac ctgtctgcca gtcatgacag tgtataatat ccggcttaaa atcaataagc
      181 gggagcattg aaagtacagc cttgtcaaag aatgtatact tctcaatctc aaacttagtg
      241 tcagaagtat atggagaaaa tcctgtaaaa aactcctgat tgtcaataaa gtaatatgta
      301 atgccgtcct gctcatattt gagcactccg acatacttgt cctgcacata tggacctgag
      361 ctcatgtaaa aatgggtaac atactcaaaa ttattgcgat actgctccgg tatacagcta
      421 taatccggta ttacaaccct tacatcccaa tagttcttgt caaactcttt aggaagtgca
      481 ccgcacacat ctgcaagacc tcctgttttt ataaatggta cacactctga agctacaaat
      541 agaatctttt tcatattctc ctcccgctaa aacccatgat atataatatt tagtttactc
      601 ttcccaattg tggtatactt cctggacgtc gtcatcatca tcaagaaggt ctaagatgcg
      661 gccgatattc gtgatatcag cttcatctgt cagtgtgaca tatgtctgtg gaatcattgt
      721 cacctctgct gaagccattg tgatacctgc ctcctcaaga gcagtcctga ctgcatcaaa
      781 atcagcaggt gcagttgtga tctcgtagct gtcatcctca tcggcaaaat cctcagcacc
      841 tgcatcgagt gcctgcatca taaggtcatc tgcatccata tcgcactctt ccttgtcgat
      901 gatgatctga cccttctcgt caaacatgta tgatacacag ccctgtgtac cgatgcttcc
      961 ctgtcccttt gtaaaggcat tgcggacatt tgccgctgta cggttcttat tgtctgtaag
     1021 acactttacg ataatagctg tgcctgatgg tccatagccc tcatatgtag ctgtctcata
     1081 gtttacactg ttgccgtctc ctgccgcctt tttgatacct ctttcgattg tatcgtttgg
     1141 catattgttg gcctttgcct tggcaactac ctgtgcaagc ttgaagttat tggcaggatc
     1201 cggtcctccc tctttaactg caactgcgag ctctctaccg atcattgtaa agattttgcc
     1261 ctttgcagca tcatttttct ctttcttatg cttgatattc gcaaatttag aatgtcctga
     1321 cataatttgt atcctctttt ctacttttaa aaataatcca taccttataa tatcattttt
     1381 tctcttccgg cgcaagtttc ttaatcatta cctgcttaat caccttatcc tcgacgctca
     1441 taacagaaaa tctgtagcca tatgcgctaa tcacagtctt atccccctcc tccggaatct
     1501 tatccgaaag cgatatgata aaaccgttaa gcgtctcgta ggcatcatca tcaacaggca
     1561 gtgacagcgc ctccttcaca tcagaaaact cagtcatgcc gtccattata aatgtctcgt
     1621 catcattctt tctgatgtag ttttcttcct catcgtgctc atcctcgata tttccgacaa
     1681 gctcctcgag tatatcctcc atcgcgataa gtcccgatat ctgtccgtac tcatccacaa
     1741 ccatcgcaag atgactcttt ttagactgca tctttttaaa cagatcattg atgtttaccg
     1801 tctcgggtat gaagtccacc ggcctgataa gcccgtctat gtccttgata ctgctgcggt
     1861 aaacctcatt tttttcaaaa aatgtaaaag catccttgat atgtaagact ccgatgatac
     1921 tgtcaacatc attctcatag accggaaagc gagatttgcc ggtatctatg ataaattcga
     1981 ttgcatcaat aaaggacata ctgccgtcaa ggcttacgat atttttgcgg tgagtcatga
     2041 tatcttttac ttccttatca tcaaactcaa acacattgtg tatcagctct gcctcagatg
     2101 caaggattgt tccctgctca tgcccctccc tgaccatgga aataatttct tcctctgtga
     2161 catcatcatt tccaagtatt ccatcgagag tctttttgtt gtttttcatt ccataaagca
     2221 atactccaat acacaaaaga agtatcacta caagtattga taaaattata attgcatata
     2281 acataatgtt ttattatcct atgtaataag ttctaagctg atttcaagcg ccctgtctat
     2341 tttaagcaat gtgtcctcat caagatggca caccttatcc ttaagccttt ttttgtcgat
     2401 tgtgcgaagc tgttctaaaa gcacaaccga atcctttgca agcgcatact ccttgcaggc
     2461 aagctccaca tgtgtgggca gctttgcttt atgcatctgc gaggttattg ctgcgcagat
     2521 taccgtcggg ctgtgtctgt ttccgacatc attttgtatt ataaggacag gtcttatccc
     2581 gccctgctct gagcctacaa caggccttaa atcagcgtaa taaatatctc ctctttgaat
     2641 aatcatacac gaatacactc catattatgg tatacaaaaa gtatctccag atttttcgag
     2701 tgtattctta aaagttacac ttttttagtt tttgtggtag attcttggaa cacgcggtgt
     2761 gataagacat gcaaactcat agttgaatct gcctgaaagc tcacctaact gctccatagt
     2821 gattgtctca tccccatctt taccgagcag agtaactgca tcgcctattt tcacgtcagg
     2881 tatgtctgtc acatcaacca taaactgatc catacagact ctgccgcata taggtgcttt
     2941 ttttcctttt atgagaaccc agcccttgtt tgaaagtcct cttgaatatc catcaccata
     3001 tcccaccggg acagttgcta tcctcatatg atgctctacc gtaaatgtgc cgccgtagct
     3061 tatatgcctg cccggctcaa gctcctttac ataagcaacc ctgctgtgca gtgacatgac
     3121 aggctttagg gagattttac tcttgtctac ctcctctgac ggccacagac catagagtgt
     3181 gatgcctgct cttaccatat ccatattgca ctcagggatt tctactattc ccgcactgtt
     3241 tgagcagtgc ttgattggaa tctcgactcc tcttgcctca acaagagcta tcattctctt
     3301 aaaaagatca aactgctcaa aggcaggctc ctttgacagc tcatctgccc ttgcaaagtg
     3361 agtgaatatt ccctctatca taatattcgg cagcttagca atctgtgcaa taatatctgc
     3421 actttcctct gtcacctgaa agcctatacg gcacatgccg gtatctatct tgatatgtat
     3481 cttacaggtt ttattcttag cgacagcgat acgcgatagc tccttagctg tctcatatgt
     3541 acataccgcc gggcgcacat cattgtccac tatctcctca aactgctccg ggaagctgag
     3601 tcctaaaata agtatcggct ttttcctgcc tgccgcaata agctgcattg cctcgttgct
     3661 tgtagcaacg gcatagcccc acagatacgg aagcggctcc agtacctctg aaacagcctc
     3721 agcgccatga ccgtaaccat cagcctttac gactgctgcc atcattgtcc ccggctttag
     3781 cttattatgc atcgactcca tattatataa aattgcatcc agatctatat ctgctctgat
     3841 tctgttaatt gccatttttt tccaccttaa tccaaacttt atctaatcca tcaataatat
     3901 cacttgctat cataccataa gagccggtat tgtcaaatac catatcaccg gcaagcccat
     3961 gaataaatgc accgtatgcc gcggcctttt tcgtgtcacg ccactgtacc agaagacctc
     4021 ccacgatacc gcttagcaca tctccgcttc cggctgttgc cataccgcag ttgccggaca
     4081 gattgataaa cttttcgcca tcagccgctg cgataatcgt acgaaaatcc ttaagtacac
     4141 aagtcacatt gtacaagctt gcaaactctc ctgccacacc gatgatatcc tcctgtatat
     4201 tagcaatgct ctttttcata agtctggaca tctcgcccag gtgcggagtc acgatgacag
     4261 gcattttatg ctcattaagt aaagtcatgt tgtctgatat gatattaagc gcatccgcat
     4321 caatcacaag aggtatctct gctgttttta gtactgtttc aactaacatt ttggcattat
     4381 cagactttga aagacctgga cctatcacca ctgcatctgc ccactcaagc tctgtctcaa
     4441 gcgctttagc tgacgcgata tcagtacaaa gcagtgcttc cggcaaagca cattggatta
     4501 ttgagcggtt gcactccggc gttataattt tcacaagccc tgctccgctt ttgtatgcag
     4561 cctttgccga aagcaccgcg gcaccggcca tattgcggct tcctgctata actaacagtt
     4621 ttccgaaagt tcccttgttg gagtgtgccg gtctgttatc aagcttcttt aaatcagact
     4681 catcaagagc ccttactgat ggcagattac cacaaaagct gtggtcatct atgcctatag
     4741 gcaccacctt aaccagaccg caataatcac agcccggcca caggagaagg cccgtcttct
     4801 tataagaaaa agtatatgtg aaatctgctt taaaagtgca tcccatcaca tgtcctttat
     4861 cagaatccac tcccgacggc acgtcgaccg ccaccctgta agcatctgtc ttgttaacgt
     4921 atgaaatcac atctgctaaa gagccttcta tggctcgctt aagccctgtg ccaaacagtg
     4981 catctattat tacgtcactt ttacacacac actcaatatc agagactacc ttaaagccat
     5041 accttgcgta tatatttttc tgcagctcaa agagctcgct tcctgcctca tctgtgccaa
     5101 aagcatagta caccatgcat ctgtatccgg cctgatttaa aagtcttgct atcgcgatac
     5161 cgtctcctgc attattgcct ctgccacata tcacggcaaa ctcctttgat ttatcaaaac
     5221 atgcaacaag ctcccggaca acaccggccg cagcctgctc catcagcacc accgaaggaa
     5281 ccaaaaagac ctgtgatgtt gtatcgtcca gaagcttcat ttcattacct gtaacaatat
     5341 attccaatat caattcccct tattctcatt gtttgcatta tcagtatttg cattattggc
     5401 attgaaaacc ataggatcgt catattcaat atcaaaaaga tctaaaacct cagcgccaaa
     5461 aatatcatga agatacgaat aaatctccct gttgttaata tctctgttct gttttctgat
     5521 tacatttttc ttaagttcaa ctcttatatt tgcaatccag tcgtcaattt cttttgattc
     5581 cagtttattt ttctcgatct tttcactgaa ataatccatc gataaaagca tcagcctgtc
     5641 atttacagct tttatattct tctggatttc aataagctct ccctcaatgt tgtcagcttt
     5701 cccatttatc tcatctataa gctgcttatc cttgtcacga ttttcatcgc cgtcatctcc
     5761 catgttggca acaatattag acataatttt cgacttaagc ttttttaaat tcttaagctc
     5821 attgttgtac tttccctgct gctttaacag ctcatccagt tctttttccg tggctgatat
     5881 ttcgtcagtc ttaccatgaa tggcaaaaag tctgtgccac ttctggtcta aaaccagtaa
     5941 aggcaccttt tttcctttta atgcagcctc aaaatctttt ttatcattca taagactcac
     6001 cttctattta tttttggagt ttttaacaat attatagctg agctgcataa ggctgtcaga
     6061 cagatgaaca ctctccacaa ccacatcttt atttggaatt tcaagatcca catgtgcaaa
     6121 attccatata gcccttattc ctaaatccac aagctttgac gcaatagagt cagcctttga
     6181 ctttggcatg gtgagcgccg caatatccac cctgtgtgtt ctgcaatact catccagctc
     6241 acttagcata aggattggaa tacctcgcac actctttcct gaaagctcag gatttacgtc
     6301 aaaaagcgct gttatgacaa agccaagctt ttcaaacttt acataattgg cgagtgcctg
     6361 gcccagattg cctgcaccga tgataattat tctatgctct gtgttaagtc ccattatctt
     6421 tccgatttcc tcgtagagaa actgaacatt gtatccgtat ccctgctgtc caaagccacc
     6481 gaaattattg agatcctgtc tgatttgtga agctgtcact ttcattctgt gactcagatc
     6541 gtttgatgaa atacgctcca ctccctcatc caaaagctct ccgagatatc tatagtatcg
     6601 tggcagtctt ctgataactg cctgtgatat ttctttactc aagtcttatt cctccaatca
     6661 tcaggatatc atttctcttt taaagacaaa aacccaagat taattataat tttttgaagg
     6721 tcatctgtca atgtgttaag aatttaacat tcaaaaaatt cataattatc cccgggttat
     6781 atcttattct aaggctaaaa accaaatttc agattaaatt gtcttctcaa gttcctctct
     6841 gattgccttg atgaagcctt cgctgtttaa aacagtagga ttttcaattg ttgtgatgag
     6901 tgcgagatcc tttgtcatct ttccgctctc aatagtcttt atgcaggctg cctcaagctt
     6961 gtctgcaaac tcagaaagtg ccggaatctt gtcaagctct cctctttttc tgagtgctcc
     7021 tgtccatgca aaaatagtag ctacagagtt tgttgatgtc tcctcaccct ttaagtgctt
     7081 gtaatagtgt ctctgtactg ttccgtgtgc tgcctcatac tcatagtatc cgtgagggga
     7141 tacaagcact gatgtcatca tggcaagtga gccgaatgca gatgagatca tgtcactcat
     7201 tacatctccg tcgtagttct tgcatgccca gataaatcca ccctcagatt tcattactct
     7261 ggcaactgca tcatcaataa gtgtgtagaa atatgtaatt ccagccttct caaaagcatc
     7321 cttatactgt gcatcaaaga tttcctggaa gatatccttg aaggtgtggt catatttctt
     7381 tgagatggta tcctttgtag caaaccataa atcctgcttt gtatcaagtg catacttaaa
     7441 gcagctatgt gcaaagctct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnngt tccgtcttct gctgtaaata caagctcagc ctttccggga ccggctacct
     7621 tcatctctgt tgccttgtat acatcaccat atgcatgacg agcgattgta attggtttaa
     7681 cccatgtctt tacgtttggc tctat
//
LOCUS       DPUF01000105            6522 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4726, whole
            genome shotgun sequence.
ACCESSION   DPUF01000105 DPUF01000000
VERSION     DPUF01000105.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4726"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            180..>535
                     /locus_tag="DHV81_03770"
     CDS             180..>535
                     /locus_tag="DHV81_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin transporter"
                     /protein_id="HCI93624.1"
                     /translation="MRNEKVQKLTLTALMAALSYVIFTFLQIKVTLPGGSATSFHLGN
                     AVVVLAALLLGGVYGGIGGAIGMTIGDLLDPIYITGAPKTFFLKLMIGVITGLVAHKL
                     GKITYSTDKKHVFKWTV"
     assembly_gap    536..652
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <653..823
                     /locus_tag="DHV81_03775"
     CDS             <653..823
                     /locus_tag="DHV81_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin transporter"
                     /protein_id="HCI93625.1"
                     /translation="DMLILGKPMAQLKIAWSVTTTGVNAVISVIAATIVYVALRPVLI
                     KANLFFKIGKEK"
     gene            1198..1764
                     /locus_tag="DHV81_03780"
     CDS             1198..1764
                     /locus_tag="DHV81_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="HCI93626.1"
                     /translation="MFILEGKLTFINIEQSYLKKLHDACSEVYYKPSGYENKPYIGIL
                     INKNDRKYVIPLSSAKEKHKTWKNVDKECYLVYEMAKKSSMAQGDIWVAAEEDDVKHI
                     LSVMDIKKMIPIVDGVYSRVNINPDDTDTDDVKKYKDLLNKEYSFCLKVIDEVIGRAN
                     KLYDKQMATGKVVKFCCDFKALEEVADK"
     gene            complement(1841..2503)
                     /locus_tag="DHV81_03785"
     CDS             complement(1841..2503)
                     /locus_tag="DHV81_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCI93627.1"
                     /translation="MNTQNIYNNNNKDININSNTKVKICGLTSPAEARYLNENHVDFA
                     GMVLFFPKSKRNISIEQAKEIMAALDASIKRVAVVVSPSIEQVRQIEAAGFDYVQIHG
                     EIPETEAEAAKAIPILKAFNVSDMDSYEKYHNDSRIAGYVFDAIEPGSGKTFDWKLVD
                     NIPRDEKLLLLAGGLNPDNVRMAIEAVHPDGVDVSSGVENDDKVGKNPEKIHDFVAAV
                     KS"
     gene            2978..4037
                     /locus_tag="DHV81_03790"
                     /pseudo
     CDS             2978..4037
                     /locus_tag="DHV81_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741189.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M42"
     assembly_gap    3244..3287
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4097..4477
                     /locus_tag="DHV81_03795"
     CDS             4097..4477
                     /locus_tag="DHV81_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCI93628.1"
                     /translation="MNLKKVHHIAIIGSNYEKSKHFYVDLLGFSIIRENYRPERDDYK
                     IDLQLDEIELELFIIKNCPKRPSYPEAYGLRHLAFAVDSVDDTVRELNKRGIITEPIR
                     LDTYTGKRMTFFHDPDNLPLEIHE"
     gene            4512..4931
                     /locus_tag="DHV81_03800"
     CDS             4512..4931
                     /locus_tag="DHV81_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCI93629.1"
                     /translation="MEYRKFDNIIIARIDKGEEILEQLKAIAITENIKLASINALGAI
                     NDFTVGVYKTDEKKYYSNSFKGYYEITSLTGTINTMDGEYYAHLHLSAGNEKGEVFGG
                     HLNRAVVSATCELVITVIDGKVDRVYDEETGLNVFNF"
     gene            4979..5467
                     /locus_tag="DHV81_03805"
                     /pseudo
     CDS             4979..5467
                     /locus_tag="DHV81_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            5595..6239
                     /locus_tag="DHV81_03810"
     CDS             5595..6239
                     /locus_tag="DHV81_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCI93630.1"
                     /translation="MKKRITLIVFSVLIIVALYVLYCFNYIPHKKYTNADFNIEAYKS
                     NIDKDNDGIDDQTDILNNANNYIKTNPKYKSKYYNTGYPDDEYGVCTDVVAFALKDAG
                     YDLMVLVNEDIKNNKELYDIDAVDKNIDFRRVKNLKVYFDNNAISLTTDINEIEEWQG
                     GDIVVFKKHIGIISDKRNRKGICFVIHHANPYQIYYEEDILEHRDDIIGHYRIS"
     gene            6296..6508
                     /locus_tag="DHV81_03815"
     CDS             6296..6508
                     /locus_tag="DHV81_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93631.1"
                     /translation="MWWSIGGGIIIFALGMFIFLKPGLIWKLTEEWKSYRADEPSEFY
                     LKTTKIGGILFALFGIIMIMLPVILE"
BASE COUNT         2118 a         1027 c         1421 g         1795 t
ORIGIN      
        1 tcgaagcaga gccagcaggt gataatagaa atgctgaaaa aacactatag cggaattaaa
       61 ccataggtgt aaatggactt tagataaact ggatatataa atttatataa aattggatat
      121 atacttgatg ccacataaga gtattatata gtttaattgt aatatttagg aggcatttta
      181 tgagaaacga aaaagttcaa aaactcacac ttacagcact tatggcagct ttatcatatg
      241 tcatattcac atttttacag attaaggtca cattaccggg tggcagcgcc acatcctttc
      301 accttggcaa cgcagtcgta gtacttgcag ctttgctctt aggcggagta tatggaggaa
      361 tcggtggagc aatcggaatg acaatcggtg atttacttga cccgatatat atcaccggag
      421 caccaaagac atttttcctc aagcttatga ttggtgttat caccggtctt gtagcacaca
      481 agcttggaaa gattacatat tcaaccgaca agaagcatgt atttaaatgg acagtnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatatgct
      661 tatcctcgga aagccgatgg cacagctcaa aatcgcatgg agcgtgacca caacaggagt
      721 caatgcagtg atatcggtga ttgctgcaac aatcgtgtat gtggcactga gaccggtgct
      781 catcaaggca aatctgtttt tcaagattgg caaagaaaaa taatagttac ataatattta
      841 gcattaaaac ctgattatga tgagacgaga tgaaaataca aatatattcg acagagtagc
      901 tgaaataagt ttataccatc ggtttcttcg ttacaagctg acgtgtgaat actaatggac
      961 aaaatataat caatatataa tcaatatata atactgcagt tgacatagct tgttaattgt
     1021 catattataa ttatagagag gtctccatta accttatggt cttgaatcgt agaagagatg
     1081 aacattaaag aaggtctcca ttaaccttcg gtaatcaatc cgtaaagaga tgagcattaa
     1141 cgagtgggaa gcttaggctt cccacatttt taatctaaaa tataaaggag cagtattatg
     1201 tttatactag agggaaagtt gacatttatc aacattgaac aaagttacct caaaaagctg
     1261 catgatgcat gctcagaggt gtattacaag ccaagcggtt atgagaataa accatacata
     1321 gggattttga taaacaaaaa tgacagaaaa tatgtaattc cattatcctc agcgaaagag
     1381 aagcataaga catggaaaaa tgtcgataaa gaatgctatt tggtttatga aatggcaaag
     1441 aagtccagta tggcgcaagg agatatttgg gtggcagcag aagaggatga tgtaaagcat
     1501 attctttctg ttatggatat taagaaaatg attccgattg tggatggtgt ttacagtagg
     1561 gtaaatatta atccagatga cacagatacc gatgatgtca agaaatataa ggatttgcta
     1621 aataaagaat attctttttg cttgaaggta attgatgaag ttatcggaag ggctaacaag
     1681 ttgtacgaca aacagatggc aaccggaaag gtggttaagt tctgttgtga ttttaaggca
     1741 ttagaagaag tggcagataa atagtggcgg atggctcgca tgagccatcc gtttacaact
     1801 atatagaggg aggtatttag tgtcgtgcag ttgtcatatt ttatgattta actgccgcaa
     1861 caaaatcatg tatcttttcc ggattcttcc ccactttgtc atcattttcc acacctgatg
     1921 atacatcgac tccgtcagga tgcacggctt caattgccat gcgcacattg tctggattta
     1981 agccacctgc gagcagcagc agcttttcat cgcgggggat attgtcaacc agcttccagt
     2041 caaaggtttt tccactgccg ggttcgatgg catcaaacac atagcctgcg atgcgtgaat
     2101 cgttgtgata cttttcatag ctgtccatat cagagacatt gaaggcttta agaatcggga
     2161 tagcttttgc tgcctccgcc tctgtctccg gaatttcccc atgtatctgg acatagtcaa
     2221 agcctgcagc ctcaatctgt ctgacctgct ctatggatgg cgaaactaca acggcgactc
     2281 ttttgatgga agcatcaagg gctgccataa tctcctttgc ctgttcgatg gatatatttc
     2341 tcttactctt tggaaaaaat aacaccatac ccgcaaaatc aacgtgattt tcgttgagat
     2401 accgtgcttc ggcaggtgaa gtcagaccgc agatttttac tttggtgttg gaatttatat
     2461 ttatatcttt attgttatta ttatatatat tctgtgtatt catatcctaa gaatagcatt
     2521 gttgcaggtt gatcgcaagt ggatatatgt taaattaatg agcattaatt gcaagtggat
     2581 ttacgttaaa ttattgagta tagttcgcaa gccgcgggta ccctggagca cacactatcc
     2641 cgcgaaagcg aatagcggag ctgcttagag actgtagaat tgtgctatgt aaagcagaaa
     2701 tgccgaggca tggacgccga ggcaaggaga aatcgagcca tggatggcga gtttcgacgg
     2761 tttcgtcgag ttaagaaaac cttgggcaca attcgtacag cctcttagct gcgtagcgta
     2821 gcgaacgtgg gatagtgtgt gcaccatggt acccgcggtt tgcgaacagg tgtactacta
     2881 caaacaacta caaacaacgg ttaatacgga ttgcatgcgc aaaaatgaga tgttataatg
     2941 cagttaataa acagtaataa agcaggagat aacaattatg tcaacagaag agattaaaga
     3001 atatatcgga acacagctca aagcactcac atcaatagca agccctacag gtttcacaaa
     3061 aaatgccaca gactatctga tgaagcagct tgaggctatg ggatatgcgc cacagctttc
     3121 aaataaagga aatgtcagcg tggaaatcgg tggagaggga gcaactttgg tattggcagc
     3181 gcatgtggac acacttggag ccatggttcg ctcaataaag gataacggac gtttgcgccc
     3241 gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt catcagtgga
     3301 gcacagcaga tggagaaaac tgcatggttt ttacgcgtga tggcagaatg tacacaggcg
     3361 tggtgctaaa tacagagcca tcggcacatg tggcagacga aaaggttgag acaaaggaag
     3421 agaatatgga aatcctgctc gatgaaaatg taaatgacaa gcagggcgta gcggcacttg
     3481 gcatccagac aggggatatt atcgccatgg atccacgtac agtcatcacg gagagcggct
     3541 atatcaagag ccgtttcctt gatgataagc tgtcagccgc catcttactc ggtgtggctc
     3601 atgccgtaaa ggaggagggc tggaagctaa accgcaaggt cacactgctc tttacggttt
     3661 atgaggaggt aggccatggc ggaagctttg taccggctga tactgaggag atgatttccg
     3721 tagacatggg ctgcgttgga gccgacctcg gctgtacgga gcatatggtt tcaatctgtg
     3781 cgaaggactc aggcggacct tacaactacg agcttgttac agagctttca aatctggcaa
     3841 agagcgaggg gcttgactat gcaatcgatg tttatccgca ctacggctct gatgttgagg
     3901 caacactgca cagcggatat gatatccgtc atgggcttat cggtgcagga gtttatgcat
     3961 cgcataacta tgagcgctca catatggatg gcgtggagaa tacctataag cttttgaaaa
     4021 aatatattac aaaatagtat cattgtgata cattatttca tgtattcaaa caaagtagga
     4081 gtgaggtaaa tacaccatga atttaaaaaa ggttcatcat attgccatta ttgggagcaa
     4141 ctatgagaaa tcaaagcact tttatgtgga tctgttaggt ttttctatta ttcgagaaaa
     4201 ttacagaccg gaacgtgatg attataaaat tgatcttcaa ctcgatgaga tagaattaga
     4261 actttttatc attaaaaatt gtccgaagcg tccaagttat ccagaggctt acggtctcag
     4321 gcatcttgcc tttgctgtag actcggtcga tgatactgta agagaattga ataaaagggg
     4381 catcataact gagccgatta gactcgatac ttacacaggc aaaagaatga cattctttca
     4441 tgatccggac aatctaccct tggaaattca cgaataaaga ataacaattc gaaagttacg
     4501 gaggaaacat aatggaatac agaaaatttg ataatataat aatagcgaga attgacaaag
     4561 gggaggagat tttggagcaa ctgaaagcaa ttgccataac tgagaatata aagcttgcca
     4621 gtattaatgc tcttggagct atcaatgatt tcacagtcgg agtatacaag acagatgaga
     4681 agaaatatta ctcaaacagt tttaaaggct attatgaaat aacatcgctg acaggtacaa
     4741 tcaatacaat ggatggagaa tattatgcac atcttcattt gagtgccggt aatgaaaaag
     4801 gtgaagtgtt tggaggacat ctgaacagag ctgttgtaag tgcaacttgc gagctcgtta
     4861 ttacggtaat tgatggaaaa gttgacagag tttatgatga agaaacagga cttaatgtat
     4921 tcaatttctg aacacttttt ggtttttcat aatagtatct ataactgttg ttatttttat
     4981 ttgtggtcga tattattttg cacacaataa aagctataaa aatgaggcaa ttgattatgc
     5041 aaaatgggag aactttgcaa aagtaattaa aagagcaatg atcgcatgtg aaaacagcgg
     5101 gcacagtatt ccagacgatt ttcctgaagt caggaaaatc gttgaagctg gagtcaccac
     5161 gaaccccaaa aaagattatg aactgtcaag atatgcatgt tatctgatcg ttcagaatgg
     5221 agatccaaga aaagaggtca ttgcattagg acagacttat tttgcaatcc agacatacag
     5281 gcaggaggtt gctgaccatt ttaatgaact ggacgaagat aacagaagac ttgttgtcag
     5341 gggagacatc aaacagtgga atcaaatgtt ggcagagaca gcacataatg caggagttat
     5401 tacgaatgag gaatttgcga tttttcaaaa tgccggatat atgggactat atggtggatt
     5461 agatgttgaa ggtattttgg cagcagttta tcagagtgtg tttggtggag atgtatatct
     5521 gtcattagag gaaaaagtag ctaatttatt atttaatgaa attggtaact cgcaagttgt
     5581 ttaatggaga atgtatgaag aagagaataa cgcttattgt tttttcggtg ctaataattg
     5641 ttgctttata tgtgttgtat tgttttaatt atattccaca taaaaaatat acaaatgcag
     5701 atttcaatat agaagcttac aagagcaata ttgataaaga caatgatggt atagatgacc
     5761 aaacagatat attaaataat gcaaataatt acataaaaac taatccaaaa tataaaagca
     5821 aatattacaa cacaggttat ccagatgatg aatatggggt ttgtactgat gtagtagctt
     5881 ttgctttaaa agatgcagga tatgatttga tggtattagt aaatgaggat ataaaaaaca
     5941 ataaagaatt atatgatata gatgccgttg ataaaaatat agattttaga agagttaaaa
     6001 acttaaaagt atatttcgat aataatgcaa taagtcttac tacagatata aatgaaatag
     6061 aagaatggca aggtggagat attgttgtat ttaaaaaaca tatcggcatt atatctgaca
     6121 agagaaatcg aaaaggtata tgttttgtta ttcatcatgc aaacccatat caaatatatt
     6181 atgaagagga tatactggaa catcgtgatg atatcatagg acattataga atcagttaaa
     6241 caactcccag tttttgaaac tgagaaatcg gaatttgcta aaggagatgg taaatatgtg
     6301 gtggtctatt ggtggtggaa tcataatctt tgcgttaggt atgtttatct tcttaaagcc
     6361 gggcttaata tggaaattaa cagaagaatg gaaatcttat cgtgcagatg aaccatctga
     6421 gttttacttg aaaacaacaa aaataggtgg tattctattt gccctatttg gaattattat
     6481 gattatgctt ccggttattt tggagtaata aattcaagtt tg
//
LOCUS       DPUF01000106            3271 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_19345, whole
            genome shotgun sequence.
ACCESSION   DPUF01000106 DPUF01000000
VERSION     DPUF01000106.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_19345"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="DHV81_03820"
     CDS             complement(<1..281)
                     /locus_tag="DHV81_03820"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569515.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCI93632.1"
                     /translation="MSKITMTTPLVEMDGDEMTRILWQMIKDELLIPFIDLKTEYYDL
                     GLEERNRTDDQVTVESAKATMKYGVAVKCATITPNAARMTEYNLKEMWK"
     gene            complement(295..954)
                     /locus_tag="DHV81_03825"
     CDS             complement(295..954)
                     /locus_tag="DHV81_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI93633.1"
                     /translation="MSHDNYSLSARVYNHIRDGILAGTFAKGDELKEKNIGDELGVSR
                     TPVREALRQLELEGLVSIIPNKGAFVEGVSTDDIRDIYEIRALLEGLCARWAATRISD
                     ELMAAMEENIYLTEFHEKKGNAKKLLELDNRFHELLYEAGGSKELMRVLKDYHEYVGR
                     VRKVTLGETKRAKNSTHEHKQICEAIKNHDALLAEKCAREHINNSKKNMEHLGWENLI
                     K"
     gene            complement(971..2353)
                     /locus_tag="DHV81_03830"
     CDS             complement(971..2353)
                     /locus_tag="DHV81_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCI93634.1"
                     /translation="MREVLMNEKTNLLQLEEHFYQLVDVDEPNTFRNLFPYSEVPKIA
                     FNDRIVPHNMPEDIWITDTTFRDGQQSRAPYTTEQIVTIYDYLHKLGGPKGIIRQSEF
                     FLYSKKDRDAVYKCLERGYKFPEVTSWIRASKKDFELVKDIGLKETGILVSCSDYHIF
                     YKMKMTRREVMNMYLSVIRECLETGISPRCHLEDITRSDIYGFVIPFCVELMKLQEEY
                     KIPIKVRACDTMGYGVNFPGAVIPRSVPGIIYGLNTHAGVPSSQIEWHGHNDFYKAVN
                     NSTTAWLYGASGVNCSLFGIGERTGNTPLEAMVFEYAQLKGTLDGMDTTVITELSEYF
                     EKELGYVQPSRTPFVGRNFNVTRAGIHADGLLKNEEIYNIFDTGKFLNRPPLVAVSNT
                     SGLAGIALWINTYYRLPDDRKVDKNSKLVTMIKKWVDEQYDEGRVTTITDNELVVQIT
                     DCCKKLNIVL"
     gene            2502..2753
                     /locus_tag="DHV81_03835"
     CDS             2502..2753
                     /locus_tag="DHV81_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorion class high-cysteine HCB protein 13"
                     /protein_id="HCI93635.1"
                     /translation="MSDLAATNCSCSNTDNGCGCSSILWIILLLCCCCGNGNGGSFFG
                     GNDCGSGNNSCIWAIILLFCCGGFGCGSSNNGCGCGCGC"
     gene            3024..>3271
                     /locus_tag="DHV81_03840"
     CDS             3024..>3271
                     /locus_tag="DHV81_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93636.1"
                     /translation="MDENSLFTPFDEITQTRELQMLKTIIPYMPHDSQKQMTLMVHYI
                     SLMNSMRMIDCAPALSVAETESMSDRRLSMLNALKKYC"
BASE COUNT          812 a          784 c          607 g         1068 t
ORIGIN      
        1 ctcttccaca tctcctttaa attgtactct gtcattctgg ctgcgtttgg tgtgattgtc
       61 gcacatttta ctgcgacacc gtatttcatg gttgcctttg ctgattcaac ggtcacctga
      121 tcatctgtac gatttcgctc ctctaagccc agatcataat actctgtctt taaatcgata
      181 aaaggtatta aaagctcatc ctttatcatc tgccagagga ttctggtcat ctcgtctccg
      241 tccatctcta caaggggtgt tgtcatcgta atcttgctca ttcttaaatc tcctttactt
      301 aataaggttc tcccaaccaa ggtgttccat atttttcttt gaattgttta tatgctctct
      361 ggcgcatttt tctgccagca gtgcgtcatg attcttaatt gcctcgcata tctgtttatg
      421 ctcgtgagtg gagtttttgg ctctttttgt ctcgccgagt gtcacctttc tcactctgcc
      481 gacatactca tgatagtcct ttaataccct cataagttcc ttgcttccgc cggcctcata
      541 cagcagttca tgaaacctat tgtccagctc aagaagcttc tttgcattgc cctttttctc
      601 atgaaactcc gtgagataaa tattctcctc catggccgcc atgagctcgt ccgatatcct
      661 cgttgcagcc catctggcac aaagtccctc aagcagagcc cttatctcgt atatatccct
      721 gatatcgtcc gtggaaacac cctccacgaa cgctccctta ttggggatga tagacacaag
      781 tccctcaagc tcaagctgac gcagtgcctc cctcacggga gtcctgctca ctccaagctc
      841 atcaccgata ttcttttcct taagctcatc gccctttgca aatgttcctg caagtatacc
      901 atccctgata tgattgtata ctctggcact caggctgtaa ttatcatgtg acatgtaata
      961 atccacctca ctataatact atattaagct ttttacagca atcagtaatt tgaactacaa
     1021 gctcattatc tgtgatggta gtcacacggc cctcgtcgta ctgctcatct acccatttct
     1081 ttatcatggt cacaagcttt gaattcttat ctacttttct gtcatccggc aggcggtagt
     1141 aagtgtttat ccacagcgct attcctgcaa gtcctgatgt attggacaca gctacaagag
     1201 gtggtctgtt taagaattta cctgtatcga atatgttgta aatctcctca tttttgagaa
     1261 gtccgtcagc atggattccg gctctcgtca cattgaagtt tctgccgaca aaaggtgtac
     1321 ggctcggctg cacatatcca agctccttct caaagtactc tgacaactct gtgataaccg
     1381 ttgtatccat tccatcgagt gtgcctttaa gctgtgcata ctcaaatacc atagcctcaa
     1441 gtggtgtgtt tccggttctc tctccgatac caaacagtga acagttgaca ccgcttgctc
     1501 cgtagagcca tgccgtagtc gaattgttta ctgccttgta aaagtcattg tgtccatgcc
     1561 actcaatctg tgatgaaggc acacctgcat gtgtgttaag tccgtatatg atccccggca
     1621 cgcttcgagg tataactgct cccgggaagt ttactccata tcccattgta tcacaggcac
     1681 gcaccttaat cggaatcttg tactcctcct gcagcttcat gagctcaaca cagaacggaa
     1741 taacaaaacc atatatatca gaacgtgtga tatcctccag atggcaccta gggcttattc
     1801 ctgtctcaag gcactccctg atgactgaca gatacatatt cattacctcg cgtctggtca
     1861 tcttcatctt atagaaaata tgataatccg aacagcttac cagtataccg gtttccttga
     1921 gtcctatatc ctttacaagc tcaaagtcct ttttgctggc acgaatccac gatgtaacct
     1981 ccgggaactt atatcctctc tcgagacact tgtaaacagc gtccctgtcc tttttgctgt
     2041 agaggaaaaa ctcgctctgt ctgattattc ccttagggcc accaagctta tgtaaataat
     2101 cataaatggt aacaatctgc tccgtcgtgt acggtgctct cgactgctgg ccgtctctga
     2161 atgtagtatc tgtaatccaa atgtcctccg gcatattgtg aggcactatt ctgtcattga
     2221 atgctatctt aggaacttct gaataaggaa agagatttct aaaagtattc ggctcatcta
     2281 catccacgag ctggtaaaaa tgctcttcaa gctgcagtaa atttgttttt tcgttcatca
     2341 aaacttctct catcttcaca tctcctgtgc ttagttttat cttttgaata attgtataca
     2401 attatacttt ttgtgtcaat aaaaaaattt aaatcttagt gtaacacttt gttatgtcta
     2461 tcataaccta gagtgtaaat aaattttatt ggaggtcact tatgagtgat ttagcagcaa
     2521 caaactgttc atgcagcaat acagataacg gatgcggatg ttcttcgatt ttatggatta
     2581 tcctgttact gtgctgctgt tgtggaaatg gtaacggagg ctctttcttc ggaggaaacg
     2641 actgtggaag cggcaataac agctgtatct gggctatcat cttacttttc tgctgtggtg
     2701 gctttggctg tggaagcagc aataacggat gcggatgtgg ctgcggctgc tgatttacat
     2761 ttttactgcg ggtcttttcg gcccgcagct ttttacgatt tttcagtatt ctttttttgc
     2821 ctgcgctgct ctttttcttt gagcttttca aggactttac attcgccctt caccctgtgc
     2881 tttttgatgc attcttcatc tatctcgtat acgtcgtgac catctatgat aagcatatat
     2941 ctttttttaa taatatatgc tgtaaacatg cataatgtga atcaggtgac atattatata
     3001 gaaatatatt ttaaaggatt tttatggacg agaacagcct gtttacacca ttcgatgaaa
     3061 taacccagac aagggagctt cagatgctta aaaccataat cccatatatg ccgcatgact
     3121 cacaaaagca gatgactctt atggttcact acatatcact tatgaattca atgagaatga
     3181 tagattgtgc accggcactg tctgttgcgg agactgaaag tatgtcagac agaaggcttt
     3241 ccatgctcaa tgccttaaag aaatactgca g
//
LOCUS       DPUF01000107           27751 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8383, whole
            genome shotgun sequence.
ACCESSION   DPUF01000107 DPUF01000000
VERSION     DPUF01000107.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 27751)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 27751)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27751
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8383"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="DHV81_03845"
     CDS             complement(<1..236)
                     /locus_tag="DHV81_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCI93637.1"
                     /translation="MNVLVINCGSSSLKYQLINSDSEEVLAKGLCERIGIDGRLVYQK
                     EGLDKEITEAPMPTHKEAIQMVLDALVNPKTGAV"
     assembly_gap    328..487
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            488..1657
                     /locus_tag="DHV81_03850"
                     /pseudo
     CDS             488..1657
                     /locus_tag="DHV81_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516138.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    1405..1495
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1659..2771)
                     /gene="aroB"
                     /locus_tag="DHV81_03855"
     CDS             complement(1659..2771)
                     /gene="aroB"
                     /locus_tag="DHV81_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCI93638.1"
                     /translation="MSIFNVELKKVVDDTYDIEIGYNLSDTLVSDLENGLAGKIKKFA
                     VITDTNVRDHYALPICEKIKHAGYSVDLFVFPAGEKSKSRETKAKIEDAMLKKGYRRD
                     CCIIAVGGGVVTDLSGFIAGTYGRGVPFINYATTLLAAADASVGGKTAIDTPLATNTI
                     GLFNQPQKVYIDIAAWKTLPQRQMASGMAETIKHACLGSEDMFEFIEENLDDIYSFKK
                     FACEYIAENNCRIKYNVVMKDEREAGLRETLNLGHTVGRAIETVSDYKLLHGEALSIG
                     MAAQALMSARLGYMSEEQAERVIKLYERAGLPTRIPDYIDREELVKKLYTDKKVRDGK
                     LRFVLQKGIGATVEFAPGVYAKPIEESLAREIIMEL"
     assembly_gap    2772..2858
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2859..3463)
                     /locus_tag="DHV81_03860"
     CDS             complement(<2859..3463)
                     /locus_tag="DHV81_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H"
                     /protein_id="HCI93639.1"
                     /translation="MASKYYAVKKGKVPGIYFNWNDCKAMVDGYPGAVYKSFKTIEEA
                     EKFINGEKIISGEKTITGMKSSGENSTYAFVDGSFNKATHTYGYGGFLVTDNEKYVLQ
                     GADNDAEMATMRNVAGEIKGAEAAVKKAIELGVKELVIYYDYKGIEMWATGDWNRNKA
                     GTIAYHEYIMSVKDKIKLTFVKVKGHSGVEGNEEADKLAKQA"
     gene            complement(3476..4216)
                     /locus_tag="DHV81_03865"
     CDS             complement(3476..4216)
                     /locus_tag="DHV81_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCI93640.1"
                     /translation="MKDNNVILEVKDLHKSFGEQEVLKGISTEVNRGDVIAIIGPSGC
                     GKSTFLRSLNLLETPTSGQILFEGTDLTDKSTDINVTRQKIGMVFQQFNLFANMTIKA
                     NIMLAPVKLGVMTKDEASKKADELLTRIGLLDKADAYPAQLSGGQKQRVAIIRSLAMN
                     PDVILFDEPTSALDPEMVGEVLSVMKELAEAGMTMVVVTHEMGFAREVANRVMFINEG
                     TIAEEGTPEEVFGAPKSERLQEFLSRVL"
     gene            complement(4206..5742)
                     /locus_tag="DHV81_03870"
                     /pseudo
     CDS             complement(4206..5742)
                     /locus_tag="DHV81_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516141.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     assembly_gap    4494..4591
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5843..6920)
                     /locus_tag="DHV81_03875"
                     /pseudo
     CDS             complement(5843..6920)
                     /locus_tag="DHV81_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568691.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
     assembly_gap    6638..6725
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6913..7764)
                     /gene="prmC"
                     /locus_tag="DHV81_03880"
     CDS             complement(6913..7764)
                     /gene="prmC"
                     /locus_tag="DHV81_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCI93641.1"
                     /translation="MTYREAILLGESILQKAKIVDAKNDAWLLLAMACRINHTYYYVH
                     MDEEMSQEQIREYQALLSKRAERIPLQYIVGEQEFMGLKFRVNSNVLIPRQDTETLVE
                     EALKVIEPGMRVLDMCTGSGCIIISILKNTTNVDGAACDISKQALNVAKENARLNGVF
                     VDFERSDLFEHVDEMYDVIVSNPPYIRSDEIPHLMPEVSVFEPHEALDGSEDGLLFYR
                     RIIKDCRANLKPQGRLLFEIGCDQGRQVSEMMQFAGFSDVHVIKDLAGNDRVVSGVFD
                     SKEEKHV"
     gene            complement(7785..8753)
                     /locus_tag="DHV81_03885"
     CDS             complement(7785..8753)
                     /locus_tag="DHV81_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HCI93642.1"
                     /translation="MKYSGIGGQAVMEGVMMQNKDTYAVAVRKPDHEIDVKVSKRKNS
                     KTLDAIRKIPILRGVVSFVDSLYLGMSTLMYSASFFEDEDDEGTLASKKKEEELTAEE
                     KKKRAAKEKKQENAMLGGTVVLSIVIALALFFALPYFLSGFFKKVISSQMLIAFIEGV
                     IRLTIFLGYIAIISLTPDIKRTFMYHGSEHKCINCIEHGLPLTVENVRKSSKHHKRCG
                     TSFLLIVMLINILFFMFIRVDSKPLQLLLRLVLIPVIAGVSYEFIRLAGRYDNPVVNM
                     LSVPGLLMQRMTTKEPDDEMIEVGIASVEAVFDWKKWQEENNVCAH"
     gene            complement(8824..9024)
                     /locus_tag="DHV81_03890"
     CDS             complement(8824..9024)
                     /locus_tag="DHV81_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCI93643.1"
                     /translation="MREGIHPDYYQATVTCNCGNTFVTGSTKPEIHVEICSKCHPFYT
                     GQQKANQARGRIEQFNKKYGMK"
     gene            complement(9195..10535)
                     /locus_tag="DHV81_03895"
     CDS             complement(9195..10535)
                     /locus_tag="DHV81_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCI93644.1"
                     /translation="MEQMREKYESLSAFVLKDLAKARGIKGVSSLKKGQIIERMLEED
                     AKEAEEAEKNGTAEERANSFKDDYAALDSGEEAEGILEVMPEGFGFIRCDNYMPGDHD
                     IYVAPAQIRRFNLKTGDIVKGNMKVKSEREKFQALLYLTTVNGYTPDVAQKRTSFEDL
                     VPIFPNERLRLERPGASVAMRVVDLISPIGKGQRGMIVSQPKAGKTTLLKEVAKSVTV
                     NNPEMHLIILLIDERPEEVTDIKEAIEGDNVEVIYSTFDELPEHHKRVSEMVIERAKR
                     LVEHGNDVMILLDSITRLARAYNVTVPPSGRTLSGGLDPVALHMPKRFFGAARNMRNG
                     GSLTILATALVDTGSKMDDVVFEEFKGTGNMELVLDRKLSEKRIFPAIDIVKSGTRRE
                     DLLLDEDEQAAVDNMRKAFNGMRSDEAVENVLNMFAHTKSNKEFVNLVKKNRIL"
     assembly_gap    10650..10737
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10830..11978
                     /locus_tag="DHV81_03900"
     CDS             10830..11978
                     /locus_tag="DHV81_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HCI93645.1"
                     /translation="MDNKEKALKCHEEWNGKLETIAKSKVNSREDLAIAYTPGVAEPC
                     KVIAEDKEAAYKYTWKANTVAVVSDGSAVLGLGNIGPYAAMPVMEGKCVLFKEFGNVN
                     AVPICLDTQDTEEIIAAVKNIAPGFGGINLEDISAPRCFEIEERLKEMLDIPVFHDDQ
                     HGTAIVVLAGIINSLRLTGKKKEDCKVVVNGAGSAGVAITKLLLTYGFTHVTMCDRLG
                     IIGKDYPNLNWMQQKMTEVTNLENAKGTLADALKGADIFVGVSAPNIVTAEMVSSMNK
                     DAILFAMANPVPEIMPDVAKAAGARVVGTGRSDFPNQINNVVAFPGIFKGALEGRAAQ
                     ITEEMKLAAAEALAGLVSDEELNDDFIMPEAFDPRVAAVVSEAVKSHI"
     gene            11980..12888
                     /locus_tag="DHV81_03905"
     CDS             11980..12888
                     /locus_tag="DHV81_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCI93646.1"
                     /translation="MRYYSLNRYCRDTFGEKLYKLSLDGGMTCPNRDGTLSSLGCLYC
                     SAGGSGDFAADRSLSIKEQLSAAKEKIASKSSCEHFIAYFQAFTNTYAPVDYLRRIFY
                     EAIEDPSVAVLSIGTRTDCFSTEIYDLLSELNEIKPVWVELGLQTIHRATLDAMNTHT
                     CVEDFIIATDELRRRGIKVIAHLILGLPHETRGMMLESVDFVAKSGIFGVKLQLLHVL
                     KGTPLADMYIKQPFELFTLDDYCDFVVDCIERLPKDMVIHRMTGDGPRSLLVAPLWST
                     DKKRVLNTINKRFEVRDTYQGRLNLF"
     gene            12900..13415
                     /locus_tag="DHV81_03910"
     CDS             12900..13415
                     /locus_tag="DHV81_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 domain-containing protein"
                     /protein_id="HCI93647.1"
                     /translation="MAEPFTIYKLTILYMLDKAGFPLSNTQISNFFLEKEYTDYFRVQ
                     EVIGDLVNADLINFESTHSNTQYTLTAAGKETLGFFKDKITDGIESDVKSFFTDNKLE
                     FRQENSILADYYRTTDRKYAVRCQVRSESTTIIDLTLSVGTKEQAEAICSNWKNQNED
                     VYAYLMDMLLR"
     gene            complement(13417..13986)
                     /locus_tag="DHV81_03915"
     CDS             complement(13417..13986)
                     /locus_tag="DHV81_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCI93648.1"
                     /translation="MGKQNWKPGNMLNPVPAVMVSVTDKAGNNNIITVAWAGTVCTNP
                     PMLSISVRPERFSYNMIKETGEFVVNLTTEELLRACDYCGVKSGRDVDKFKELHLTPL
                     AMQHVSAVGISQSPVNIECRVRKIEELGSHTMFIADVVGVTVDDKYMDGTGKFHINDT
                     GLVMYSHGEYFGFGDKLGSFGYSVRKTKK"
     gene            complement(14033..14917)
                     /locus_tag="DHV81_03920"
     CDS             complement(14033..14917)
                     /locus_tag="DHV81_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCI93649.1"
                     /translation="MSLFKGAGVALITPFNEDNSVNYEMLRTLVRRQIDGGTDAIIVC
                     GTTGEPATMTEEEKLSVIKCVVDETAGQIPVIAGTGANCTQNVIDFSQKVEKLGVDGL
                     LVVTPYYNKATQNGLYAHYAAIAGAVGLPIIMYNVPSRTGCNILPQTAARLGRDFENI
                     VGIKEASGNISQVAKLKKLAGDDLDIYSGNDDQVIPILSLGGIGVISVLSNVMPAAVH
                     DMVTKYLNGNIKSALDIQLKYLDLIEALFCEVNPIPVKAAMKLLGYDVGGLRLPLTEL
                     EDANRARLKESMENLKEN"
     gene            complement(14986..16458)
                     /locus_tag="DHV81_03925"
     CDS             complement(14986..16458)
                     /locus_tag="DHV81_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCI93650.1"
                     /translation="MKLEKLLERMDYKVLAGSTDIEISTLVYDSRKVEKASVFVCISG
                     AVSDAHDFIPDVVKKGAAAVVVEKDVTPIEGVTYIKVDDTRLALACMSAAYFDYPAEK
                     IKTIGITGTKGKTTTTYMVKSILESAGIKTGLIGTIESICGDKRIPSANTTPESYRVQ
                     ELFKEMVDEGLDAVVMEVSSQALMLHRVSGFTFDIGVFTNLEPDHIGEHEHKDFADYM
                     HCKSLLFRQCRLGIFNGDDEHLEGIMKGHTCQVETYGYKSTNNLVAENVELKKEHGAL
                     GVKYHVSGLLDFDVEVNVPGKFSVYNSLTAIAICHHFNVDKKAIGEALHHVSVKGRIE
                     IVPVTKRYTLMIDYAHNAMALESLLTTLKEYEPGRLVCLFGCGGNRAKSRRYEMGEVS
                     SRLADLTVVTSDNPRDEEPMDIINDILQGVHKADGEYVTIPDRKEAIRYCMENAKDGD
                     IIVLAGKGHEDYQEIKGVKHHMDERELIADIIKENPDIRL"
     gene            complement(16576..17433)
                     /locus_tag="DHV81_03930"
     CDS             complement(16576..17433)
                     /locus_tag="DHV81_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCI93651.1"
                     /translation="MKFTKMHGCGNDYVYVNLFEEKLDDPARVSIYVSDRHFGIGSDG
                     LITIGPSDKADFRMRIYNADGSEAEMCGNGIRCVAKYVYDHKLTDKTEISVETGAGIK
                     YLTLYVEENKVSQVRVDMGEPILTPGDIPVVKADGSAYGDDYRVIDEPISAGNREWHM
                     TCVSMGNPHAVVFVDDVAGFELEKYGPLFENHKMFPKRTNTEFVEILSRNEAKMRVWE
                     RGSAETWACGTGTCATVMACILNKKTDNKVLVHLRGGDLTIEYIPETNHVFMTGPATE
                     VFSGEIDIM"
     gene            17584..18420
                     /locus_tag="DHV81_03935"
     CDS             17584..18420
                     /locus_tag="DHV81_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI93652.1"
                     /translation="MDSNYTKAGYLTEPFKIFHLRDEHKLNIDFHYHDFHKILIHLHG
                     NVSYCIEGRSYELKEHDIVLVNAGEVHKPILNDDSIYERIIIYVSQQLIDDYVSKGYD
                     LACCFKQAYANQSHVLRLAATKGSRLADTIKELDSTLRETTEYANELYQKLLFLEFLI
                     QLNRITLHGGIEYINTFSSNKKIVEILDFLNKNLTNRISIDELANKFYMSRYHLMHTF
                     KEETGCTIGSYITTKRLLLARDMIRDGSSVSAACDACGFGSYSSFIRAYRKQFHSTPT
                     NT"
     assembly_gap    18441..18624
                     /estimated_length=184
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18625..>20072)
                     /locus_tag="DHV81_03940"
     CDS             complement(<18625..>20072)
                     /locus_tag="DHV81_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HCI93653.1"
                     /translation="DSICAAIAYANLKSHTDDTMEYIPMRAGSVSAETAFVLDYFKAD
                     TPALLKDVGTQIKDIHIRRTEGVSSNLSMKKAWEMMKILNVVTLPVVNKKDKLEGLIV
                     TGDIAKSYMDVYDNSILAKARTQYRNIIETLDGKIVAGNEHGYFVKGKVIVGAGTPDT
                     IKGNVAEDDLVIISDREESQLICIEANCSCIIVTSGFDISKDVINAANAKDVVVISTP
                     YDTFTTSRLINQSMPIKSFMTRENLIHFDLDDYVDDVRDTVSKIRHRDFPILDENQNY
                     VGMFSRRNLMNARKKQVILVDHNETSQAVANIDEAEILEIIDHHRIGSLETIGPVYFR
                     NQPLGCTGTIIYQMYMEKGVEVTPQMAGLMCAAILSDTLVFRSPTCTQVDKSTALILA
                     EIAQIDVEEFAQKMFEAGSNFANKTEEEILNQDFKIFHSGDYTFGVSQISALSRTELD
                     KVQARVVPLLDKMQVEKQLDMLFVMLTDILNES"
     assembly_gap    20073..20141
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20216..21178)
                     /locus_tag="DHV81_03945"
     CDS             complement(20216..21178)
                     /locus_tag="DHV81_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93654.1"
                     /translation="MSGIFDRRAKRFISTEQAERAVPSRTVMASRVARDDVNHSERVQ
                     KKEDNIQMDKDDFLLTQIDEFREKAKRLQEMLNTKESKAEELSTIVEEREAKAEELQQ
                     ILDERQEKADGITAQVAKQIDVLIESVHEKMAEIEQSMNAGMDSLGRQVSSDMDNLGR
                     SVNANMDNLGSSMSGQIGNLGQSLGTEISGISQNIGQSLDFGDTLEQTRRITEEGTAA
                     VTSAVNEANAHMLQSLTELNDQLVSLKAELSDKVHTENVKCFRNIQDLFKVMGDKVDT
                     VSELEKQVGTTKTFAVISTVLAIINTFGFVAIALYILGFFGSIF"
     gene            complement(21349..>21825)
                     /locus_tag="DHV81_03950"
     CDS             complement(21349..>21825)
                     /locus_tag="DHV81_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCI93655.1"
                     /translation="GKETSKKSLSEIAMSYGYVYVAQIALGANPAQAVKAITEAEAYP
                     GPSLIIGYAPCELHGIAKGGMNHCQDEMKKAVKAGYWNLFSFNPALKAEGKNPFTLTS
                     KAGDGSYQEFLNNEARYTRLIKPFPERAEKLFAKSEETAKARYTHLEKLVKLYGED"
     assembly_gap    21826..21950
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21951..24945)
                     /gene="nifJ"
                     /locus_tag="DHV81_03955"
                     /pseudo
     CDS             complement(21951..24945)
                     /gene="nifJ"
                     /locus_tag="DHV81_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443805.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     assembly_gap    23342..23422
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25230..26870
                     /locus_tag="DHV81_03960"
     CDS             25230..26870
                     /locus_tag="DHV81_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93656.1"
                     /translation="MISANNITLRIGKKALFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGQLEPTSGDIAITPGQRLSFLQQDHFKYDEFTVLDTVIMGNKRLYDIMKEKEAI
                     YMKEDFSDEDGIRASELEAEFADMDGWNAESDAATLLNGLGVPTDDHYTLMADLPGAV
                     KVKVLLAQALFGNPDILLLDEPTNHLDLDAISWLEEFLINFENTVIVVSHDRYFLNKV
                     CTNIADMDYGKIQLYAGNYDFWYESSQLIVRQMKEANKKKEEKIKELQEFIQRFSANA
                     SKSKQATSRKRALEKIQLDEIRPSSRKYPYIDFRPEREIGNDVLFVEGISKTIDGVKV
                     LDNISFTVNHDDKIAFVGGNELAKTTMFKILAGELEPDEGSYKWGVTTSQSYFPKDNT
                     TIFDTDELIVDWLTQYSEIKDATYVRGFLGRMLFAGEDGVKKMRVLSGGEKVRVLLSR
                     MMIMGSNVLMFDEPTDHLDMESITALNNGMIKFPGVILFACRDHQVVQTTANRIIEFL
                     PNGSMIDKITTYDEYLESDEMARKRQVYSMSEDDENDN"
     gene            complement(26962..27681)
                     /locus_tag="DHV81_03965"
     CDS             complement(26962..27681)
                     /locus_tag="DHV81_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HCI93657.1"
                     /translation="MRGIGMWDSSSISTLFSGFSSSRSSNAASSFIANMDMTTYSGIR
                     SGSYFKLLKSYYGNNLNSKAQSLVSSSVSTSKDSAKTLASIESESEDMVKSAQALYKN
                     SRKDDTEATYKKVSAFVSDYNSLINAADDSETKQISRNLESMKSLTDINSKSLAKVGI
                     TVDSKSGKLSVDEDTFKKADSTKVDALFKGNGSYAYAVASKASMLEYAAKNEAEKPNT
                     YGANGRYTQAYNSGYNYNMFL"
BASE COUNT         6948 a         6490 c         5148 g         8094 t
ORIGIN      
        1 tttactgcgc ctgtctttgg atttacaagt gcatcgagta ccatctggat ggcctcttta
       61 tgtgttggca taggtgcctc tgtgatttcc ttatcaagac cctctttctg atatacgagt
      121 ctgccatcga ttccgattct ctcgcagagt ccttttgcaa gtacctcctc tgaatctgaa
      181 ttgatgagct ggtatttcag tgatgaacta ccgcaattaa ttactaatac attcattgtt
      241 ttgtctcctt tggtttaaaa ttacaatact attgttagta taaacttatt tttcaaatgc
      301 gacaagggtg tttttgtggt attatggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnntag taatgagcgg tgatttcgtg caaagaggtg agcctgccat cattgacaaa
      541 tacgagcgca cacgcatggc gcttttgagc ggtgccgacc tggtgcttga gcttccatcg
      601 gtctttgcca ctgcttctgc cgagtttttt gccggtggcg gagtcagtgt actcaaaaac
      661 acaggtgtgg tggacatgct gtgctatggt gtggagtctg tggaccatga acttacaaag
      721 cttgtggctg gcgtgcttaa gaatccgcct tctgagtatt ccacctctct tgccaggctg
      781 attcagggcg ggatgagctt tcctgcggcc aggagcagag cgctatgtga atattttcga
      841 gatacctatg acagtgcttc cgagaaaata gatgctttca tagcttcacc caataatatt
      901 ctggccattg agtatgagaa ggctttgatg gactgtgata ttacaggatt tcctattcag
      961 cgcgtcgggg aaggatatca cagcacggat tcgacaagtg agttttcttc cgctacagct
     1021 gtgcgcgggg tgatttcgac cttaattgat atagacaaac acaacactat tactaatata
     1081 cagcttgata attcatggat ttccacaaag ttcagtcagc taatgccatc agactgcgcc
     1141 gatattcttg tcaattgtat tttaggcggt catattgtgt ttccggatga tatttctgaa
     1201 atgctttact atcgcctgct gacccggaag gataaaggct ttgcgcagta tgcagactgt
     1261 acaaaggagc tttctgcaaa gatagttaaa aatatttata aatatgaaag ttttacgcag
     1321 ttttgtaact tactgaagag taaaaatctg acttatacgc gtatttcaag ggttctcacg
     1381 catattttac ttggtattga gaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncttat
     1501 gcatctcctc aaaaaaagag catctgcgcc tattatttca aaagtcgctg atgctccgta
     1561 cgagcttatt tccaaggata tttttgccgc tgatttgtat ggcaggctgt gtcattcaca
     1621 gcaaaatgag tttacacatg gggttgtaat tatttaatct acagctccat tataatttct
     1681 cgtgccagac tttcctctat aggctttgca tacactcccg gtgcaaactc aacagtggct
     1741 cctatgcctt tttgtagcac gaagcgcagc ttgccgtctc tcaccttttt atcggtgtag
     1801 agtttcttta caagctcttc cctgtctata tagtcaggta tacgtgtcgg aagaccggct
     1861 ctctcataga gctttatcac gcgctctgcc tgctcctctg acatatagcc aagccgtgct
     1921 gacataagtg cctgtgctgc cattccaatc gacagtgcct ctccatgcaa cagcttgtag
     1981 tcgctgactg tctctattgc ccggcctact gtgtggccca gatttaaggt ttcgcgaagt
     2041 cccgcctctc gctcatcctt catgaccaca ttgtacttga tacggcagtt attttctgca
     2101 atatactcac atgcaaattt cttgaatgaa tatatatcat ccagattttc ctctataaat
     2161 tcaaacatat cctcgctgcc taagcatgcg tgcttaatgg tctctgccat accgcttgcc
     2221 atctggcgct gtggcagtgt cttccaggct gctatgtcta tatatacctt ttggggctga
     2281 ttgaacagac ctatggtgtt ggtagcaaga ggtgtatcaa ttgctgtttt tccacctacc
     2341 gatgcatctg ccgccgctag aagggtggtt gcatagttga taaacggcac ccctctgcca
     2401 taggttcctg caataaagcc tgataaatcg gttaccacac cgccaccgac tgcaatgata
     2461 cagcagtctc ttctgtaccc ctttttaagc atggcatcct ctatctttgc ctttgtctca
     2521 cgcgacttgc tcttttcccc cgccgggaaa acaaacaaat ccactgagta tcccgcatgc
     2581 tttatcttct cacagattgg aagagcataa tgatcacgca catttgtatc tgtaatcact
     2641 gcaaatttct ttatcttgcc cgcaagacca ttttccaggt cagatacaag ggtgtctgag
     2701 aggttgtagc ctatctcaat atcatatgtg tcatctacta cttttttgag ttctacattg
     2761 aatatgctca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ctgcttggcg agcttatctg
     2881 cctcttcgtt gccctctact cctgagtgtc cttttacctt cacaaaggta agctttattt
     2941 tatccttaac tgacatgatg tattcgtgat aagctatagt gcctgccttg tttcgattcc
     3001 agtcccctgt ggcccacatc tctatgccct tgtagtcata gtatatgacc agttccttaa
     3061 ctccaagctc tatagccttt tttacggcag cctccgcgcc ctttatctct ccggctacgt
     3121 tgcgcattgt ggccatctcg gcatcattat ccgccccctg cagcacgtat ttttcattgt
     3181 cagtcacaag aaagcctcca tagccatagg tgtgtgttgc tttgttgaag gagccgtcta
     3241 caaacgcata tgtactattt tcgccggatg atttcatacc tgtaatagtt ttctctccgg
     3301 aaataatttt ctctccgtta ataaattttt ctgcctcttc tattgtctta aagcttttat
     3361 acactgctcc cggatatccg tcaaccattg ctttgcaatc attccagttg aaatatatcc
     3421 cgggaacctt tccctttttt actgcatagt attttgatgc cattctttat ttctcctata
     3481 acactcttga caaaaattcc tgtaatctct cactctttgg tgcgccaaat acttcctcag
     3541 gtgtgccctc ctcagctatg gtaccctcat tgatgaacat cactctgttg gccacttctc
     3601 tggcaaagcc catctcatgt gtcacgacta ccattgtcat gcctgcctct gcaagctcct
     3661 tcattactga aagcacctca cctaccatct cagggtcaag tgccgatgta ggctcatcaa
     3721 acagtatgac atcaggattc atagcaagtg aacggataat tgccactctc tgcttctgtc
     3781 ctccggaaag ctgcgccgga tatgcatctg ccttatcgag gagacctatt cttgtgagaa
     3841 gctcatctgc cttctttgat gcctcatcct tcgtcatcac accaagcttc accggtgcga
     3901 gcatgatatt tgcttttatt gtcatgttgg caaacagatt gaactgctgg aaaaccatgc
     3961 cgattttctg tctggtcaca ttgatgtctg tcgacttgtc tgtcaggtct gttccttcaa
     4021 aaaggatttg tcctgatgta ggtgtctcga gcagattgag ggagcgtaaa aatgtagact
     4081 ttccacagcc tgacggtccg atgatggcaa ttacatcacc tctgttcacc tctgtgctta
     4141 tgccctttaa tacctcctgc tctccaaaac tcttgtgcag gtcttttacc tctagtatca
     4201 cgttattatc tttcattgct tctcagtctc ctctcaagtt tgcttactcc gtggcttaaa
     4261 agcatcacta taaccagata aataagggct acagcaataa gcggtataaa tgcctcgtat
     4321 gtgttgcttc gtatgatatc tccacccttt gtcagatcca tcataccaat ataaccactg
     4381 attgatgtct ccttcaggag gctgataaac tcgttaagca gcgccggaag tatatttttg
     4441 acagcctgcg gtatgataac aagcttcatg gtctgtctgt aattgagtcc taannnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagttgagg ccgaaagcaa tgactgctac
     4621 aagtatcttg ttggtcacag atgcaagcac tacatagtat ataataagaa gctgtatcat
     4681 gacaggtgtt cccctgatga tggtcaggta tgccttgcaa attccgttta atatggcaag
     4741 accgccgttt ctgtcatggc tggttctgac tatcgcaatt aaaaatccca gcacaatacc
     4801 aagcattacg gcaaatattg tgataactat tgtattgcca agacccttta cgatatacat
     4861 gtaacgattg tccttgacaa agttgtcata gagcttttcc tttataccga ttgaagaacc
     4921 cgactggctg tcttttacga taatctcctg ctttgtggtg atatatgtat cggtaaaatc
     4981 aacatttttc tgtctgtcct ctgtcactgt aaagcctgct gccccgacat ccaccttgcc
     5041 tgatgataca gcggcaatga ttgcgtcaaa ctgcatatcc tctacctgca tatccatgcc
     5101 aagctcatcg gctatggcct gcatgatatc catatcaatt cctgtgacct tgttgtcctc
     5161 atagtactca tatggaggga actctgcatt ggttcctacc ttaagtgttc cgtttgacct
     5221 atcaatatcc ttcttcacgt agggagtctt tcccacctga tcctcagagc cgatatagtt
     5281 gtcgataatg ctctgaactg ttccatcctg ctgcagcttt tcaagcgcag tgtttacctt
     5341 gtcgcgaagc tctgtattgc cctttttcag gcagaatgca tagtcctcgt tggtaaactc
     5401 ctcgtccagt atcttaagtc ctttgttttc ttttacaaag gcaagtgccg gctgctcatc
     5461 aataactacg cagtcaatct tgccgacctt taatgcctgg actgcatcag cacccttgtt
     5521 gtatctctct accttggtgt ctgaaccgtc gttctcataa tcagatacat agatatctcc
     5581 tgtggtaccg agctggacac ctatttttgc gccctccaga tcgtccacac aagcgacctt
     5641 tttgctgtcg cttttgccgc atgcgcataa gccaacaatg aatgtggcca ttaatgctgc
     5701 cataagcaaa atcataatcg gttttttctt aatggatttc atattattcc tcatttctgt
     5761 ataaatattt attcacaatt atatcatatt agtttataaa ataaaagagc ctgattttct
     5821 cagactcttt tataccaaat atttatgcat catgctccat gttgcttaac ttcttcgcct
     5881 ggtcggctgc gatacaggca tcaattattt cctggatatc tccgttaaga atcttgtcaa
     5941 gcttatagag tgtcagattg attctgtgat ctgtcacacg tccctgtggg aagttgtagg
     6001 tacgaatctt ctcagcacgg tctcctgtac cgatctggct tcgtctctcc tctgcctctg
     6061 catcctgctt cttctgaagc tccatgtcat agagctttgt acgaagaagt gcaaaagcct
     6121 tttccttgtt ctgtatctgt gacttctcag tctggctgta gatgacaata cctgttggat
     6181 aatgtgtcag tcggactgct gaatctgttg tgttgacaca ctgtccaccg tttccggaag
     6241 cacgcattac gtcaattctg atatccttgt catcaatctt gacatccaca tcctctgcct
     6301 caggcattac agcaactgag catgtagagg tctggatacg tccctgcgac tctgtctcag
     6361 gcactcgctg cacacggtgt actcccgact catactttag gatagagtat gcacctgagc
     6421 cctttaccat gaactcgaca gacttcatac cgccgattcc tgtctcatcg gcatcaagtg
     6481 tctccacctt ccagcctctg ttctctgcgt agtgcacata catacggtag atttccgctg
     6541 caaaaagtgc tgcttcctct ccgcctgcac cggcacggat ttcgacgatt acgttcttgt
     6601 catcgttagg gtcctttggc agaagaagta ttttaagnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnncaaaa tagccagtga atcctcgata ttctgcttgc actctttata tttcttatat
     6781 gtctcaacta taggagcaag gtcactctgc tccttcataa gctttttaaa tctgtttgca
     6841 tcatttgcca catccggctc gctgagcaga ttcatcagct cttcataatg gtgtataaga
     6901 tcttctaact tatcaaacat gtttttcctc cttgctgtca aacactcccg atactaccct
     6961 gtcatttccc gccaggtcct ttatcacatg tacatcgcta aatccggcaa actgcatcat
     7021 ctctgacacc tgtctgccct ggtcacagcc tatctcaaaa agcagcctgc cctgtggctt
     7081 tagatttgcc ctgcaatcct ttatgatgcg cctgtaaaat aaaagtccat cctcagagcc
     7141 gtcaagagcc tcatgcggct caaataccga cacctccggc atcagatgcg gtatctcgtc
     7201 gcttcttata tatggtggat tggatactat cacatcatac atctcatcga catgctcaaa
     7261 cagatcgctt ctctcaaaat caacaaacac gccgttaagc cttgcgtttt cctttgccac
     7321 attgagcgcc tgctttgaga tatcacaggc tgcaccgtcc acattagtcg tgttctttaa
     7381 gatgcttata attatgcagc cgcttcctgt acacatatca agcactctca tacccggctc
     7441 tatcaccttg agtgcctcct ctacaagagt ctctgtatcc tgacgcggta tgagcacatt
     7501 gctgttcaca cggaatttga gtcccataaa ctcctgctct ccgactatgt actggagcgg
     7561 aatacgctca gcgcgcttac tcagcaatgc ctgatattcc cttatctgct cctgcgacat
     7621 ttcctcatcc atatgcacat agtaatatgt atgatttatc ctgcatgcca tggctaaaag
     7681 cagccatgca tcgttttttg catcaactat ctttgctttt tgtaatatgc tttccccaag
     7741 taaaattgct tctctatatg tcatatatac cttctatccg gcggtcaatg tgcacacaca
     7801 ttgttttcct cctgccattt cttccagtca aacacagcct caaccgatgc gatgccaacc
     7861 tctatcattt catcatccgg ctcctttgtg gtcattcgct gcattaaaag cccagggaca
     7921 ctaagcatgt tgacaaccgg attgtcatat ctgcccgcaa gtctgataaa ctcataagat
     7981 acacctgcga ttacaggaat aagcacaagt cgtaacaata actgcagcgg ctttgaatcc
     8041 acacggataa acatgaaaaa caatatgttt atgagcatta ctataagcag aaagcttgta
     8101 ccgcagcgct tgtgatgctt tgagcttttt ctcacatttt ccactgtaag aggcagtccg
     8161 tgctctatgc agttgatgca tttatgctca gagccgtgat acataaatgt gcgcttaata
     8221 tcaggggtca gcgatataat tgctatatac cccagaaata ttgtcagtct gataacaccc
     8281 tctatgaacg ctatgagcat ctgtgagctt atcacctttt taaaaaaccc tgaaaggaaa
     8341 tacggcaatg caaaaaacag cgcgagtgct atgacaattg acagtaccac tgtacctcca
     8401 agcatggcat tttcctgttt cttttctttg gcagcccttt ttttcttttc ttccgctgtg
     8461 agttcttctt cctttttctt tgaagccaag gtaccttcat catcctcgtc ctcgaaaaat
     8521 gacgctgaat acatcagtgt gctcatgccc agataaagcg aatcgacaaa gctcaccaca
     8581 cctcttaaaa tagggatctt tctaattgca tcaagcgtct ttgaattctt acgctttgac
     8641 accttgacat ctatttcatg gtcaggcttt ctcacggcta ctgcgtatgt gtccttgttc
     8701 tgcatcatta cgccctccat gacagcctga ccgccaatac ctgaatactt cataaaacct
     8761 ctctatattc caaaaaaggt tgaggtatat tgcaacctca acctttcaag taactatatg
     8821 attctatttc ataccatatt tcttattgaa ctgctcaata cgtccacgag cctggttagc
     8881 tttctgctga cctgtgtaga atggatggca ctttgagcaa atctctacgt ggatctccgg
     8941 ctttgtagat cctgtaacga atgtgttacc acagttacat gtaacagttg cctgataata
     9001 atctggatgg attccttctc tcatgatttt cacctctttt atattcttat cgtatttcta
     9061 tagcttcact ctttataccc gtatgcccga tccgcatatg atacttgctg caagagcgac
     9121 acgttgcgtc cgtttacatc ccgataaaca gcttagttat tgtagcacat gaatctgtca
     9181 attgcaagac atttttataa aattctgttt ttctttacaa gattaacaaa ttccttgttg
     9241 ctctttgtgt gtgcaaacat gttgagtacg ttctcaacag cctcatcaga gcgcataccg
     9301 ttgaaggctt tacgcatatt atcgactgct gcctgctcat cctcatcaag gagcagatcc
     9361 tcacgccttg ttcctgattt tactatatct atagcaggga agattctctt ctcggaaagc
     9421 tttctgtcga gcacaagctc catgtttcct gttcccttga actcctcaaa tactacatca
     9481 tccatcttgc ttccagtatc aacaagagct gttgcaagta ttgtcaggct gccgccgttt
     9541 ctcatattac gtgctgctcc gaaaaatctc tttggcatat gaagcgctac aggatcaagt
     9601 cctcctgaaa gtgtacgacc tgatggtgga actgtcacat tgtaagctct tgcaagcctt
     9661 gttatgctgt caaggagtat catgacatca ttaccatgct ctacaaggcg ctttgctctc
     9721 tctattacca tctctgacac tctcttgtga tgctcaggaa gttcatcaaa tgtagagtag
     9781 attacctcga cattgtcccc ctctatagcc tccttgatat ctgtaacctc ctccggtctc
     9841 tcatcaataa ggagaataat aagatgcatc tcaggattgt ttacagtgac agactttgca
     9901 acctctttaa gtaatgtggt ctttccggcc ttaggctgtg aaacgatcat gcctcgctgt
     9961 ccctttccta ttggagagat gagatctaca accctcatcg caacacttgc tccgggtctc
    10021 tcgagacgca gtctctcatt cgggaatata ggcacaagat cctcaaagct tgtacgcttc
    10081 tgtgctacat ccggtgtata tccgttgaca gtggtcagat ataaaagagc ctggaatttc
    10141 tcacgctcag acttcacctt catattaccc tttacgatat cacctgtctt taaattgaag
    10201 cggcgtatct gcgcaggtgc cacatagata tcatgatctc ccggcatata attatcacag
    10261 cggataaatc caaagccctc cggcattacc tcaagtatgc cttctgcctc ttctccactg
    10321 tcgagtgctg cataatcatc tttaaaagag ttggcgcgct cctcggctgt accgtttttc
    10381 tcagcctcct cagcctcttt tgcatcctcc tcgagcattc tttcaataat ctgtcccttt
    10441 ttcagtgatg aaacgccctt aattcctctt gccttcgcca gatccttaag cacaaatgcc
    10501 gacaaacttt catatttttc tctcatctgt tccatttcgt cttctcctta aatttgataa
    10561 cagtatttat tcaattcgtc taaacaatat tatttaacaa atatatctgt gtagctattg
    10621 ggaaataacg cctgaattga cttttgaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt
    10741 ttatataatc ggttatctgc atagttactt gatttaaatc acctgcaatg ttatgattat
    10801 tcaaaagaat ttttagaggg agaattacca tggataataa agaaaaagca ctgaaatgcc
    10861 acgaagagtg gaacggaaaa ctcgaaacca tcgccaaatc aaaggttaat tcgagagagg
    10921 accttgcaat tgcatacaca cccggtgttg ctgagccatg taaggtcata gccgaggata
    10981 aggaggctgc atacaaatac acctggaagg caaataccgt tgccgtagta tcagacggaa
    11041 gtgctgtgct tggtcttgga aacatcggtc cttacgctgc catgcctgtc atggagggta
    11101 aatgcgtact tttcaaggag tttggaaacg taaacgctgt tcctatctgt ctcgacactc
    11161 aggacactga ggagattatt gctgcagtca aaaatatcgc accgggcttt ggcggaataa
    11221 acttagagga tatttctgct ccgcgctgct ttgagattga ggagagactt aaagagatgc
    11281 tcgatattcc tgtattccac gatgaccagc acggaactgc aatcgttgta ctcgccggta
    11341 tcatcaactc tctgcgcctg accggcaaaa agaaagagga ctgcaaggtt gttgtaaacg
    11401 gtgcaggctc agcaggtgtt gccatcacta aacttcttct gacatacggc tttacacatg
    11461 tgaccatgtg tgaccgtcta ggcatcatag gaaaggacta tccaaacctc aactggatgc
    11521 agcagaaaat gacagaggtt acaaaccttg aaaatgcaaa gggtactctc gcagatgctt
    11581 taaagggtgc tgacatattc gtaggtgttt ccgctccaaa tatcgtcaca gccgagatgg
    11641 tttcttctat gaataaggac gctatccttt ttgccatggc aaatcctgtt cctgagatta
    11701 tgcctgacgt ggcaaaggct gccggtgcca gagtggtcgg aaccggaaga agtgattttc
    11761 ctaaccagat aaacaacgtg gttgctttcc ccggaatctt caagggtgcc ttagaaggac
    11821 gcgctgcaca gatcactgag gaaatgaagc ttgctgccgc agaagcactg gccggtcttg
    11881 tttctgacga agaattaaat gatgatttta ttatgcctga ggcatttgac ccgcgtgttg
    11941 ccgctgttgt cagtgaagct gtaaagtcac atatttaata tgagatacta ttcactcaac
    12001 agatactgcc gcgatacatt cggtgaaaag ctctacaagc tctctttaga tggaggcatg
    12061 acctgtccaa accgtgatgg cacactctca tcgcttggat gcctgtactg cagcgccggt
    12121 ggctccggtg attttgcggc ggaccgcagc ctttccataa aggaacagct gtctgccgct
    12181 aaggaaaaga ttgcttcaaa gagcagctgc gagcacttta tcgcatattt tcaggctttt
    12241 accaacacat atgcgccggt agattatctg cggcgcatat tttatgaggc tatagaagac
    12301 ccatctgtcg ctgtgctgtc catcggcaca agaacagatt gtttttctac cgaaatatat
    12361 gatctgctct cagaattaaa cgaaataaag cccgtctggg ttgaactggg ccttcagacc
    12421 attcaccggg ctactcttga tgcaatgaat acacatacat gtgttgagga tttcatcatt
    12481 gcaacggatg agcttagacg ccgtggcata aaggttattg cacatctcat actcgggctt
    12541 cctcatgaga cacggggaat gatgcttgaa tcagttgatt ttgttgccaa aagcggtatt
    12601 ttcggagtta agcttcagct gctgcatgtg ctaaagggca caccactggc tgatatgtat
    12661 attaaacagc catttgagct ttttacgctg gatgattact gcgattttgt ggtagactgt
    12721 atagaaagac ttccaaagga tatggttatc caccgcatga ccggtgatgg accacgttct
    12781 ttacttgttg cacctctttg gagcacagat aaaaaacggg ttctaaacac aatcaataaa
    12841 agatttgaag tgcgagacac atatcaggga cgattaaact tattttaaag gagaaaatca
    12901 tggctgagcc atttaccata tataagctga caattttata catgctcgac aaagcgggct
    12961 ttccgctgtc aaacacacag atttccaatt tctttttgga aaaagaatac accgactact
    13021 tccgtgtaca ggaggtcatc ggtgatttag taaatgctga tttaattaat tttgagtcta
    13081 ctcattccaa tacacagtac actctgactg ctgccggaaa agagactctc ggtttcttca
    13141 aagacaaaat taccgacggt attgagtccg atgtaaagag cttctttact gacaacaagc
    13201 tcgagttcag gcaggaaaac tccattcttg ccgactatta caggacaact gaccgcaaat
    13261 atgcagtacg ttgtcaggtg cgctcagaga gcaccaccat catcgacctg actctctcag
    13321 tcggcacaaa ggagcaggcc gaggccatct gcagcaactg gaagaatcag aacgaggatg
    13381 tgtacgcata cctcatggat atgctgcttc gatagattat tttttagttt ttcttacaga
    13441 gtagccgaag cttccgagct tatccccaaa gccaaaatac tctccgtgcg agtacatcac
    13501 gaggccggta tcattgatat gaaatttccc ggtcccgtcc atgtatttat catccactgt
    13561 cacgccgacg acgtctgcta taaacattgt atggctgccg agctcctcga tttttcttac
    13621 cctgcactca atatttaccg ggctttgtga tattcccact gctgacacat gctgcatcgc
    13681 aagcggtgtc agatgcagtt ctttaaattt gtccacatcc ctgccgcttt tcactccgca
    13741 gtagtcgcag gctctaagca gctcctcggt ggtcagatta accacaaact ctcccgtttc
    13801 tttaatcatg ttataagaaa agcgctcagg ccttacagat attgaaagca taggcggatt
    13861 ggtgcacacc gtccccgccc atgccactgt tattatattg ttatttccgg ccttgtcagt
    13921 gacactcacc atcacagccg gcaccgggtt tagcatattg cccggcttcc agttttgttt
    13981 tcccatatgt taatttatcc tttaattttt ctttaatatc cttttatatc cactaatttt
    14041 cctttaggtt ttccatgctt tctttaagcc ttgcacggtt tgcatcctca agctccgtga
    14101 gcggcagacg aagtcctccc acatcatatc ccaaaagctt catagctgcc tttacaggaa
    14161 tcggattcac ctcacaaaac agtgcctcta ttaaatccag gtatttaagc tggatatcaa
    14221 gtgctgattt aatatttcca ttcagatatt tcgttaccat atcgtgcact gcagccggca
    14281 ttacattaga cagcactgat atcacaccga ttccaccgag tgagagtatt gggatgacct
    14341 ggtcatcatt tcccgaataa atatcgaggt catctcctgc cagttttttt agctttgcaa
    14401 cctgcgatat attgccactt gcttccttga ttcctactat attttcaaaa tccctgccaa
    14461 gccttgccgc agtctgtgga agtatattgc agcctgtgcg cgacggtacg ttgtacataa
    14521 tgattggaag cccaaccgcc cctgctatcg ctgcatagtg cgcataaaga ccattctgtg
    14581 tggctttatt gtagtaaggt gtgacaacaa gcagaccgtc tacaccaagc ttttcaacct
    14641 tctgtgagaa atctatcaca ttttgtgtgc agttggcacc ggttcccgct attacaggaa
    14701 tctgcccggc tgtctcgtct accacacatt ttattaccga aagcttttct tcctctgtca
    14761 ttgtggcagg ctcacctgtc gtgccacaca cgataattgc atcagtgcca ccgtctatct
    14821 gccttctcac cagcgtccta agcatttcat agtttacact gttatcctca ttgaaaggag
    14881 taattaacgc tactccggcg cccttaaaca gagacatata tacctcccaa atacactatt
    14941 ttactgaact agtttatact aaactattta acctatacta aaatattaaa gcctgatgtc
    15001 aggattttct tttattatat cagcaattaa ttctctctca tccatgtgat gcttcacacc
    15061 ctttatctcc tgataatcct catgtccctt tcctgcaagc actatgatat caccatcctt
    15121 ggcattctcc atgcagtatc tgatagcctc cttacggtca ggaattgtca catactctcc
    15181 atctgccttg tgtacaccct gtaaaatatc attgattata tccataggct cctcatctct
    15241 tggattatcg gatgtgacaa cagtaaggtc tgcaagtctc gatgacacct ctcccatctc
    15301 ataacggcgt gactttgccc tgtttccgcc acaaccaaac aggcatacaa gtctgcccgg
    15361 ctcatactcc ttaagagtgg tgagaaggct ttcaagtgcc atcgcattgt gtgcataatc
    15421 aatcataagg gtatatctct ttgtaacagg aacaatctct attctgccct ttacactcac
    15481 atgatgaaga gcctccccga ttgccttttt atccacattg aaatgatggc agatagcaat
    15541 ggctgttagt gaattgtata ctgagaattt gcccggcaca ttgacctcca catcaaaatc
    15601 caaaagccct gatacatggt atttaacgcc gagtgctcca tgctcctttt taagctcaac
    15661 attttctgct accaggttgt ttgtggactt gtagccgtaa gtctccacct ggcatgtatg
    15721 tcccttcata ataccctcta agtgctcatc atctccgtta aagataccaa gcctgcactg
    15781 tctgaataaa aggctcttgc aatgcatgta gtctgcaaaa tccttgtgct catgctctcc
    15841 gatgtggtca ggctcaagat ttgtaaatac accaatatca aaggtaaagc ccgatactct
    15901 gtgaagcatg agagcctgtg atgacacttc cataacaact gcatcaaggc cttcatccac
    15961 catctcctta aacagctcct gaactctgta tgactcaggt gtggtatttg ctgacggaat
    16021 tctcttgtca ccacatatag actctattgt accgataagt cctgtcttta tgccggctga
    16081 ctcaagaatt gactttacca tatatgtggt agtggtctta ccctttgtac ctgtaatacc
    16141 gatagtcttt attttctcgg ccggataatc aaaataagct gctgacatac acgcaagtgc
    16201 aagccttgta tcatctacct taatatatgt caccccttcg ataggagtca catctttttc
    16261 gacaaccact gccgctgcac ctttctttac cacatcaggg atgaaatcat gtgcatctga
    16321 cacagctccc gatatgcaca caaataccga tgccttctcc accttgcgcg agtcatacac
    16381 gagtgtggaa atctctatat ctgtgcttcc tgctaatact ttataatcca ttctttcaag
    16441 taatttttca agcttcattt tattttctcc catattttat tatatttttg atatatattt
    16501 tctgttaatt ttgtttctta aaaatttgta actattcaac cacagacatt cgccaaaccg
    16561 cacaatactt gtgctttaca tgatatcaat ttctcctgag aaaacctcag tagcaggtcc
    16621 cgtcataaac acatgattgg tctcaggtat atactcgata gtcagatctc cgcctcttag
    16681 atgcacaagc accttgttgt cagtcttttt gttgagtata catgccatga cggtcgcaca
    16741 ggttcctgta ccgcaggccc atgtctcagc gctgcccctc tcccatactc tcatttttgc
    16801 ctcattcctt gaaagtatct ctacaaactc cgtgttggtg cgctttggaa acatcttatg
    16861 attctcaaaa agcggaccgt atttctcaag ctcaaaacct gccacatcat cgacaaacac
    16921 taccgcatga ggatttccca ttgaaacaca ggtcatgtgc cactctctat tgcctgctga
    16981 aatcggttca tctattaccc tgtagtcatc accataagca cttccgtctg ctttcacaac
    17041 cggaatgtcc ccaggcgtca gaataggctc acccatatcc acgcgcacct gtgatacctt
    17101 attttcctca acatatagtg taaggtactt gatgcctgct cctgtctcaa ccgatatttc
    17161 agtcttgtca gtaagtttgt ggtcatacac gtatttcgcc acgcagcgta tgccgtttcc
    17221 gcacatctcg gcttccgaac cgtctgcatt atatatcctc attctaaaat ctgccttatc
    17281 agacggacct attgtgatta gtccgtctga gcctattcca aaatgcctgt cactgacata
    17341 tattgacacc cttgccgggt catccaactt ctcctcaaaa agatttacat atacataatc
    17401 atttccacag ccgtgcattt ttgtaaattt catagtgatc acctcgttat tggttttata
    17461 ttggtaacta tattatacag cgcactttct tgtttttcta tagcattttg tgcgttttat
    17521 gttgcgtttt gtgctgttat agattatgct tatagtaaca tcttattatc aaaggagcat
    17581 atcatggatt caaattatac aaaagcaggc tacctgacgg agccttttaa aatatttcat
    17641 ctaagagatg aacacaagct aaatatagat ttccattacc atgatttcca caaaattctg
    17701 attcatctgc acggcaatgt atcctactgc atagagggcc gcagctatga gctcaaagaa
    17761 catgatatcg tgcttgtcaa tgccggcgag gttcataagc caatattaaa tgacgattca
    17821 atttatgaaa gaatcattat atatgtgtca cagcagctta ttgacgatta tgtgagcaaa
    17881 ggctatgatc tggcatgctg tttcaagcag gcctatgcta accagtcaca tgtactaaga
    17941 ctcgctgcga ccaagggaag tcgtctggct gatacaatca aggaactaga ctctacactt
    18001 agagaaacca ctgaatacgc aaatgagctg taccagaagc tcctgttttt agagtttctg
    18061 atacagctca atcgtatcac cctgcatggc ggtatcgagt atattaacac attttcctca
    18121 aacaaaaaaa ttgtagagat tttggatttc ttaaataaga atttaacaaa ccgtataagt
    18181 attgatgaac tggcaaataa attctatatg agcaggtatc acctgatgca caccttcaaa
    18241 gaagaaaccg gatgcaccat aggttcatat atcacaacaa agcgtctgct gctcgcccgc
    18301 gatatgattc gcgacggaag cagtgtgtct gccgcctgcg acgcctgtgg attcggaagc
    18361 tattcctcat ttatccgtgc ataccgcaag caattccatt ctacccctac aaatacataa
    18421 aagcttcacg atatatcaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnngactca ttaagtatat ccgtgagcat aacaaagagc
    18661 atatcaagct gtttttccac ctgcatcttg tcaagcagag gcaccactct tgcctgtacc
    18721 ttatcgagct ctgttctcga aagagcagaa atctgtgata ctccaaatgt ataatcccct
    18781 gaatgaaaga ttttaaaatc ctgatttaat atttcctctt ctgtcttgtt agcaaagtta
    18841 ctgcctgcct cgaacatttt ctgggcaaac tcttctacat ctatctgcgc aatctccgcc
    18901 aggataagtg ctgtagattt gtcaacctgg gtacatgtag gcgaacggaa cactagtgtg
    18961 tcagagagta ttgcagcgca cataagcccc gccatctgag gagtaacctc cacacctttt
    19021 tccatataca tctgatagat gatggttccg gtacatccaa gcggctgatt tctgaaatac
    19081 acaggcccta ttgtctcaag agaaccaatt cgatgatgat caattatctc aagaatctca
    19141 gcctcatcaa tgttggccac tgcctgtgag gtctcattat gatccacaag tatcacctgt
    19201 tttttccttg cattcattaa atttctgcgt gaaaacatac ccacataatt ttggttttcg
    19261 tcaagtattg ggaaatccct gtgtcggatt tttgaaacag tatcacgcac atcatccaca
    19321 taatcatcca aatcaaaatg aataagattt tcccttgtca taaatgactt tatcggcata
    19381 ctctggttta tcagacgcga tgtggtaaac gtgtcatatg gtgtacttat cactaccacg
    19441 tccttagcgt ttgcagcatt tatgacatcc ttcgatatat cgaagccgct tgtcacaatg
    19501 atacagctgc agtttgcctc aatacatata agctgtgact cttcacggtc tgatatgatg
    19561 accagatcat cctcagccac atttcccttt atggtatcag gtgtacctgc tccaactata
    19621 acctttcctt tcacaaaata gccatgctca ttgcctgcaa ctatcttgcc atccagcgtc
    19681 tctataatat ttctgtactg ggtcctcgcc tttgccagga tagaattgtc atacacatcc
    19741 atatatgact ttgctatatc acctgtcaca ataagccctt caagcttgtc cttcttattg
    19801 acaaccggaa gagtgactac gttgagtatc ttcatcatct cccatgcctt tttcatggat
    19861 aaattactac tgacaccctc agtccttctg atatgtatat cctttatctg tgtgccaaca
    19921 tcctttaaaa gggcaggcgt atctgcctta aaataatcca gcacaaatgc tgtctctgca
    19981 ctgacgctgc ctgctctcat tggtatgtat tccattgtat catctgtatg tgatttaagg
    20041 tttgcatacg ctattgctgc acagatagaa tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgccttat aaaatgtcaa
    20161 aatatggtgc aactccctaa ccgggaactg caccacccta ttacaataat atttattaaa
    20221 aaatcgaacc aaagaacccc aatatataca atgcgattgc aacaaaacca aatgtattga
    20281 taatagccag cacagtagag atgactgcaa aagtctttgt tgttcctacc tgcttctcaa
    20341 gctctgacac tgtgtccacc ttgtctccca ttaccttaaa gagatcctga atattcctaa
    20401 agcacttaac attctctgta tgtaccttgt cagacagctc cgcctttaac gaaacaagct
    20461 gatcattaag ctctgtaagc gactgaagca tatgggcatt cgcctcattt accgctgatg
    20521 taactgctgc tgtgccctcc tctgtgatac gccttgtctg ctctaatgtg tcaccaaaat
    20581 caagactctg acctatattc tggctgatgc ctgatatctc agtgccaagt gactgtccga
    20641 gatttccaat ctgtccggac atcgaactac caagattgtc catattggca tttaccgagc
    20701 gtccaagatt atccatatct gagcttacct gacggcctaa gctgtccatg cctgcattca
    20761 tagattgctc tatctcagcc atcttttcat gaacactttc tataagcaca tctatctgct
    20821 tagcaacctg tgcagtgatt ccatcggctt tttcctgtct ctcgtcaagt atctgctgca
    20881 gctcctctgc cttagcttcc ctctcctcaa ctatggttga aagctcctct gcttttgatt
    20941 ctttcgtatt gagcatctcc tgaagacgtt ttgccttttc acgaaactca tcaatttgtg
    21001 ttaacaaaaa atcatcttta tccatttgta tattatcctc ttttttctgc actctttcac
    21061 tatgattaac atcgtctctt gctacacggg atgccattac tgttcttgaa ggtactgccc
    21121 gctccgcttg ttctgtagat ataaatcttt ttgcacgtct atcaaatatg cctgacattc
    21181 gtattcctcc gatggttcaa ttaaatacca ttaacatata tattgtacca ttacatattg
    21241 tatttgtaaa taaaaaaacc acgaaatata gtgtaatcac aggagaaaaa gcaaaaatcc
    21301 cacagggcaa atgccctgtg ggataaaata tataccgtga ctaatgaatt agtcctctcc
    21361 gtataattta acgagtttct caaggtgtgt ataacgtgcc ttagctgtct cctcagactt
    21421 agcaaagagt ttctctgctc tctctgggaa tggcttgata agacgagtat aacgtgcctc
    21481 gttgttaagg aactcctggt aagatccgtc gccggccttt gatgtaagtg tgaatggatt
    21541 ctttccttca gccttgagtg cagggttgaa tgagaagagg ttccagtatc cagccttaac
    21601 agccttcttc atctcatcct ggcagtggtt cataccaccc ttagcgatac cgtgaagctc
    21661 gcaaggtgcg tatccgatga tgagtgatgg tcccggataa gcctcagcct ctgtgatagc
    21721 cttaacagcc tgtgctgggt ttgctccaag agcgatctgt gcaacgtata catatccgta
    21781 gctcatagcg atctctgaaa gtgacttctt gcttgtctct ttaccnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccagaagcaa
    21961 gtacatggtc taatccaccg aaaccgatat catatgccca tccgtcacca ccgaagatcc
    22021 atacagattt ctttccgagg taatccttct tagcaagtac ttctttagat ttctcacatc
    22081 cggctgctgc tgccttctct aactctgcaa taagagctgt tgttggctca acatttgcct
    22141 ttgtgtcatc ctttgtctca aggaacttat ctgctgctgc cttaaactca gctgtagcct
    22201 tgtctgatgc agcgatctct ttaacaagct cgatagcctg gtcacggaga tatctctgtc
    22261 caacttccat acctaaacca tgctcagcat tatcctcgaa gagtgagtta gaccaagctg
    22321 gtcccttctt agagttgata tttactgtgt atggtgatgt agctgccggg ttaccccaga
    22381 tagaagaaca tcctgttgcg ttagagatgt acatctgctc accgaagagc tgtgtaataa
    22441 gtctagcata agatgtctca gcacatcctg cacatgagcc tgagaactca aggagtggct
    22501 ggttgaactg tgatccctta ggtgttgtat caaccattcc ggcatctgtc ttcttagaaa
    22561 cgttagctac taagtaatca aatactggct gctctgctgc ctgagactcc tgtggctgca
    22621 ttgagatagc tgctgtagga caaactgtga tacactcacc acatcccata cagtcaagtg
    22681 gagatacgct cattgtatac ttgtaagcgc ctgcctttgg cttcatatca gcaaccttca
    22741 tgttgtcagg cgctgccttt acttcgtcat ctgtaagtaa gaatggacga attgtagcgt
    22801 gagaacatac aaatgcacag ttgttacact ggatacactt ctctggatcc cactgtggaa
    22861 ctgtaacggc tgttcctctc ttctcgtatg cagaagcacc tgttacatac tgtccgtctg
    22921 ccttgtcagc gaatgctgaa accggaaggc tgtctccatc catcttagcg attggatcaa
    22981 gaagctcgtt aacaagctta acaacctctg gacgtcctgt gcgctctgcc ttaggagcat
    23041 ctgcctctgg attcttccaa gactcaggaa cctctacctt atgtacagcg tcaacaccgg
    23101 catcgatagc cttgtagttc atctctacta ctgcatcacc cttctttgag tatgacttct
    23161 tagcagcagc cttcatgtac tctacagcct tatcgatagg cattacgtca gcaagcttga
    23221 agaatgcaga ctggagaatt gtgttagttc tcttacccat tccgatctcg atagccttgt
    23281 cgatagcgtt gattgtgtaa agctgaatgt tgttgtcagc gatatatttc ttagcgtcag
    23341 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23401 nnnnnnnnnn nnnnnnnnnn nnggcttaac atcctgaacc atcttcatgc cctggataac
    23461 atatgcaggg ttgtggcatg ctacgaagtc tgcctggttg atgtagtatg ggcttctgat
    23521 tgggttgtca ccaaatctca agtgagaaat tgtaacacca ccagtcttct ttgaatcata
    23581 ctggaagtat gcctggatgt atttgtctgt gtggtcaccg atgatctttg tagagttctt
    23641 gttagcacct acagtaccat ctccgccgag accccagaac ttgcactctt ttgtaccagg
    23701 tgcagatgtg ataggagctg gcttaacctc tggaagtgaa agatttgtaa catcatccac
    23761 gattccaatt gtaaatctgt gcttaggagc atccttctca agctctgtat atacagcaaa
    23821 tactgaagaa ggtggtgtat ccttagatcc taatccgtaa cgtccgccta ctaatacaac
    23881 atcgttcatt cctgcttcac gaagtgttgt agcaacgtct aagtataatg gctcaccaag
    23941 tgatcctggc tcttttgtac ggtctaatac agcgatcttc tttgctgtct ttggaatagc
    24001 attaagaaga gcctctgaag accatggacg gtaaagacgt accttaataa gtccgacttt
    24061 ctctcccttt gctgttaagt aatcgattac ttcctcagct acatcattga tagaacccat
    24121 agcgatgatt acacgatctg cgtcaggtgc tccgtagtag ttgaataatt tgtaatctgt
    24181 accaagctta gcattaacct tgtccatgta cttctcaaca actgcaggaa gctcatcata
    24241 gaacttgtta catgcctctc tatgctggaa gaagatatct ccgttctcgt gtgatccacg
    24301 agctgctgga tgctctgggt taagagcgtg tgctctgaat ttggcaaccg catccattgg
    24361 acacatctct ttgagatctt cataatccca cttctcgatc ttctgaatct catgagaagt
    24421 acggaatcca tcgaagaagt tgatgaatgg aacctgtccc tcaagtgctg cacagtgtgc
    24481 aaccggtgaa agatccataa cctcctgtgg atttgtctca caaagcattg cgaaaccagt
    24541 ctgacgacaa gcatatacat cactgtgatc accgaagata ttcagtgcat gtgaagatac
    24601 tgtacgagct gatacgtcaa atacgcaagg aagattctca cctgcgattt tgtacatatt
    24661 agggatcata agtaaaagtc cctgtgaagc tgtaaatgtt gtagtaagtg caccagctgc
    24721 aagagatccg tgaactgtac cagcggcacc tgcctcagac tccatctcaa ctacctttac
    24781 agtgcttccg aaaatgttct tctgtcctgc tgctgaccac tgatcaactg tatcagccat
    24841 tgggctagaa ggtgtgatag gatagattcc ggcaacctct gtgtaggcat atgctacatg
    24901 agcggcagcg gtatttccat ccatcgactg ttttgctcta gacatgatat ttcctcctgt
    24961 aaaataaatg ttcaattata gacatgaatc agaatgtctc tacataatag tttagtttgt
    25021 gcgctgtaaa aaatcaagtt tttttagcaa tacattgtac aaaaacccta aaatatttat
    25081 taacaaggta aaatttatgt acaaaagggc acaatggcta taaatcgtct gtaaaacact
    25141 tgcaaaatac tggttttacg ttaaaataga aacgatgtgt tacagaaacg aacaggttat
    25201 taaataggaa aggattgaat aaagacaaaa tgattagtgc aaacaatatt acattaagaa
    25261 ttggtaaaaa agctttattt gaggatgtca acataaaatt cacagaggga aactgctacg
    25321 gacttattgg tgcaaacggt gccggcaagt ctacattttt aaagattctc tcaggacagc
    25381 ttgagcctac aagcggtgat atcgcaatca ctccgggaca gagactgtca ttcttacagc
    25441 aggatcactt caagtacgat gagtttacag tacttgatac ggttatcatg ggtaataagc
    25501 gtctctatga tatcatgaag gagaaggaag ccatctatat gaaggaggat ttctcagacg
    25561 aggacggaat ccgtgccagc gagcttgagg ctgagtttgc cgatatggac ggctggaatg
    25621 ctgagtcgga tgcagctact ctcttaaacg gtctcggtgt acctactgat gatcactaca
    25681 ctctgatggc agatctgccg ggtgcagtaa aggtcaaggt gcttctcgct caggcactgt
    25741 ttggaaatcc tgacatactg cttctcgatg agcctaccaa ccacttagac ctcgatgcca
    25801 tctcatggct tgaggaattc ctcatcaatt tcgagaacac ggtcatcgtt gtatcccatg
    25861 acagatactt cctcaacaag gtctgcacga acattgccga catggattac ggtaagatcc
    25921 agctctacgc aggaaactat gatttctggt atgaatcaag ccagcttatt gtccgtcaga
    25981 tgaaggaagc aaacaaaaag aaagaggaaa aaatcaagga gctgcaggaa tttatccagc
    26041 gtttctctgc caatgcctct aaatcaaagc aggctacatc aagaaagcgt gccctcgaaa
    26101 aaatccagct tgatgaaata cgtccatcaa gccgtaaata tccatacatt gatttccgtc
    26161 ctgagcgtga aatcggaaac gatgtactct ttgtcgaggg catctcaaaa accatcgacg
    26221 gcgtaaaggt acttgacaat atctcattca ctgtcaacca cgatgacaaa attgcatttg
    26281 tcggtggaaa cgagcttgca aaaacaacta tgtttaaaat ccttgccgga gagcttgagc
    26341 ctgacgaggg ctcatataaa tggggtgtca caaccagcca gtcatacttt ccaaaggaca
    26401 ataccacaat ctttgatact gatgagctca tcgttgactg gctcacacag tactcagaaa
    26461 tcaaggacgc cacatatgtg cgaggtttcc ttggccgtat gctctttgcc ggtgaggatg
    26521 gagtaaagaa aatgcgtgta ctctccggag gagaaaaggt tcgtgtactg ctctcccgca
    26581 tgatgatcat gggaagcaat gtgctcatgt tcgatgagcc tacagaccac ctcgacatgg
    26641 agtcaataac agcattaaac aacggaatga taaaatttcc gggagtaatc ctctttgcct
    26701 gccgtgacca ccaggtagtc cagacaaccg ccaaccgaat catagaattc ctgccaaacg
    26761 gttcaatgat cgacaagatc acaacctatg acgaatatct tgagagcgat gagatggcac
    26821 gtaagcgtca ggtctactca atgagcgagg acgatgaaaa cgacaactaa acgctagtta
    26881 attaaaaaga agcatcccaa accgggatgc ttcttttatt aagccgtaaa gtgaggtggg
    26941 ggattcccag cctgtctttg actataaaaa catattataa ttatatccac tgttataggc
    27001 ctgtgtatac cttccattgg cgccatacgt gttaggcttt tctgcctcat tctttgcggc
    27061 atactcaagc attgaagcct tgcttgccac tgcgtaagca taagagccat ttcccttaaa
    27121 aagtgcatca accttggtcg aatcagcctt tttaaaggta tcctcgtcga cagacagctt
    27181 gccggacttg gaatcaactg tgattcccac cttcgcaagc gactttgaat tgatatctgt
    27241 aagtgacttc atagactcaa gatttctcga aatctgcttt gtctcggaat catccgcagc
    27301 attaatgagg ctgttgtaat cagacacaaa agctgaaacc ttcttgtacg tagcctctgt
    27361 atcgtccttg cggctgtttt tgtaaagagc ctgcgccgac tttaccatgt cctctgactc
    27421 cgactcgatg gacgcaagtg tcttcgctga atccttagat gtagaaacag atgatgagac
    27481 aagagactgg gcctttgaat taaggttatt tccataatat gacttgagaa gcttaaaata
    27541 gctgccactt ctaatgccgc tatatgtagt catatccata ttggcaataa aagacgatgc
    27601 tgcattgctg cttctactgc tgctaaagcc ggaaaacagt gtggatatgg atgacgaatc
    27661 ccacattcct attcctctca tagtatcact cctttgataa tcatgtaatc cgttaccttc
    27721 tatatatttt taatatagac gcttttccaa t
//
LOCUS       DPUF01000108           19405 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_11243, whole
            genome shotgun sequence.
ACCESSION   DPUF01000108 DPUF01000000
VERSION     DPUF01000108.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 19405)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19405)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19405
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_11243"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="DHV81_03970"
     CDS             <1..579
                     /locus_tag="DHV81_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93658.1"
                     /translation="MNILLAYYNLIDDWKDDKSLTKKTYAEMLKKDFEKAKKGYPIQA
                     KAIEDYIARLAECEKSNDTNIDAVAGLTGEMLGILFAWKQDEWQKDLKEFGCYMGKFI
                     YIMDAYEDIGDDVKKKSYNPLIQLMHCCNNDQEKFDKSCRLMMTSMLSEAAKIFERLP
                     ILLHADIIRNVLYSGVWSKYEYIQMKKRKKHK"
     gene            576..1298
                     /locus_tag="DHV81_03975"
     CDS             576..1298
                     /locus_tag="DHV81_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCI93659.1"
                     /translation="MIDPYQVLGVSRSASDDEIKKAYRSLSRKYHPDANINNPNKDQA
                     EEKFKQVQQAYDQIMKEKQSGAAGAGGYGGAGSSSSYGGSSYGGAYGSGPGAGDPFGA
                     GGPFGGFYGRYTYNNAGSSAQDDNPKVRAAGNFISNGYYNEALKVLEEVPFAERRGRW
                     YYYSAIANQKLGNTATAIEHIQRAVSLEPNNMQYRQFMQIIENGGSWYSDMGDIYGRP
                     SGRGSECCSLLATFLCWQMFCC"
     gene            complement(1516..1977)
                     /locus_tag="DHV81_03980"
     CDS             complement(1516..1977)
                     /locus_tag="DHV81_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93660.1"
                     /translation="MSVNESQIKNTTAGAIDDDDAVIFKERKRLLFLGLPWTFTKYTI
                     TPSFITVESGLLSTTEDDCYMYKVADVKLTTSLMERIFKLGTIVCYTGDVTNPELKLV
                     HVKHSREIKSYLLKQSEAARLKRRTLNTVDIGAHGADMADLDHDGIPDSME"
     gene            2178..3098
                     /locus_tag="DHV81_03985"
     CDS             2178..3098
                     /locus_tag="DHV81_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCI93661.1"
                     /translation="MRHLMSPLDFSVEELDKLLDLANDIEAHPEKYAHKCDGKKLATC
                     FYEPSTRTRLSFEAAMLNLGGSVLGFHSADSSSASKGESVSDTIRVISCYADICAMRH
                     PKEGAPLVASEKSRIPVINAGDGGHQHPTQTLADLLTIRSIKGRLNNITIGFCGDLKF
                     GRTVHSLINALIRYDNVRIVLISPEELKVPDYVRDDVLRANNIEFKEVEKLEDAMGEL
                     DVLYMTRVQKERFFNEEDYVRLKDFYILTKEKMELAKDDMIVLHPLPRVNEISVDVDD
                     DPRACYFRQAQYAVYVRMALILTLLGLAED"
     gene            3110..3541
                     /locus_tag="DHV81_03990"
     CDS             3110..3541
                     /locus_tag="DHV81_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCI93662.1"
                     /translation="MLNISGIHEGFVLDHIKAGMSLQIYRDLKLDKLDCTVAIIKNAK
                     SSKMGKKDIIKVECPIESLDLDILGFIDHNITVNVIKGDKIVDKMELKLPHQVKNVIK
                     CKNPRCITSIEQELDQIFVLSDEKNEIYRCKYCEEKYEGVK"
     gene            3550..5811
                     /gene="priA"
                     /locus_tag="DHV81_03995"
     CDS             3550..5811
                     /gene="priA"
                     /locus_tag="DHV81_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCI93663.1"
                     /translation="MYANVIVDISHEKLDKTFQYSIPDEILCDIRPGVCVDIPFGSRT
                     ITGYVIEVTDKPEYDPARTKPLIGIKADSVAVEAKLIALAAWIRRNYGSTMNQALKTV
                     IPVKKQAKEQVSRSIALKIDKVKARAQLALCEAKKQKAKARLFRALIDADDGARLEYS
                     FVTGKLSVSAATIRALEQSGFIEVITQSGYRNPVEHMSVDTYDKVLNAAQQSVVDAIE
                     GDIAAGLRNTYLIKGVTGSGKTEVYMELIAHCIQSGRQAIVLIPEIALTYQTVMRFFA
                     RFGNRVSIINSRLSNGERYDQFERAKNGDIDIMIGPRSALFTPFSNLGLIIIDEEHEN
                     SYKSESVPRYHAREVAIEYARMSDAIVVLGSATPSVDSYYKAKTGVYRLLELDKRVDD
                     RPLPKCEVVDLRQELREGNRSILSTRLQELMEERLLNGQQTMLFLNRRGKSAFMSCRA
                     CGFVVKCPHCDVSLSEHSGGVMVCHYCGYRQPVPKVCPSCGSKYISGFKAGTQKIEAM
                     VAKRFPQARILRMDYDTTRAKDAYEKILQAFSNHEADILIGTQMIVKGHDFSNVTLVG
                     VLAADMSLHVNDFHAAERTFALLTQAAGRAGRGKLPGNVVIQTYDPDHYAIRTAKEQD
                     YEAFYDKEIEYRRLMNYPPVWNMLLVHVTSPDESECGSMAQQVYDIASQMISHTDENQ
                     SPDDRHLIQLIGPADATIAKVNDIYRKVIYMKTSDYAALISIKDGIEQAVKADTAMKH
                     ANISFDFNPMSGF"
     gene            5833..6309
                     /gene="def"
                     /locus_tag="DHV81_04000"
     CDS             5833..6309
                     /gene="def"
                     /locus_tag="DHV81_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCI93664.1"
                     /translation="MALRTIRVEGDPVLGKVCREVTEVTPKIVTLIDDMLETMYEANG
                     VGLAAPQVGILKRIVVIDVGEGPIVMINPEIIESDGEQTGDEGCLSVPGKAGQVTRPN
                     YVKARFMGEDMNEYEIEGEELLARCICHELDHLDGHLYVEKVEGALHDVTYEDTQE"
     assembly_gap    6408..6479
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6480..7288
                     /locus_tag="DHV81_04005"
     CDS             <6480..7288
                     /locus_tag="DHV81_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCI93665.1"
                     /translation="MQYTPVKECALSHGIEVFQPVKIRETANIEYLRKFNADIIIVVA
                     FGQILSKSILDMPRYGCINVHASLLPKYRGAAPIQWAVINGDEFTGVTTMRMDEGVDT
                     GDMIAKSTVRLAPDETGGSLFDKLSAEGARLCVETMKMIEDGTAEYTPQNSEEATHTS
                     MISKELGFIDWTKPAVEIERLIRGLNPWPSAYTHLNGKTFKVWSAKVIDGSDDYEPGC
                     IYHIGKNDMYVQTGKGALSLVEVQLQGKKRMDTGSFLRGCHVEEGSFFTL"
     gene            7754..8466
                     /locus_tag="DHV81_04010"
                     /pseudo
     CDS             7754..8466
                     /locus_tag="DHV81_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743172.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     assembly_gap    8376..8412
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8572..9981
                     /locus_tag="DHV81_04015"
     CDS             8572..9981
                     /locus_tag="DHV81_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase"
                     /protein_id="HCI93666.1"
                     /translation="MINNHSKNNHNNKNSQINKNNIPGRPAKSNVAAHNEHSVDTREL
                     ALEMLIEISERGAFSHIVLRSVLDKYQYLSKQDRAFMTRLVDGTIEYMLQLDYIIDSF
                     SKTQIRKMKPFIRNLLRMSVYQIKYMDAVPDSAVCNEAVKLAKRHKFAQLSGFVNGVL
                     RNIARNIDSVQFDTLSIRYSMPQWIVDRFVSAYGEKKAEEIFKAFHNKSTISIRTNLT
                     RCTPDELRAMLEAEGVTVTAVDELNYAFVISGFDYLNGLQSFRDGLFYVQDISSMLVA
                     QTAAPKKGDYVIDVCAAPGGKSTHIAELLQGSGHVLARDLTDTKVDMIEENIERHGLN
                     NMSAEVWDATVPDADSAGKADILICDLPCSGLGVLGRKKDIRYKMTPESVDELAALQR
                     QILDTVHTYVKTNGVLVYSTCTIDEVENEDNVRWFIEKHPEFELDKSFAEGTGMKQIL
                     PGEHGSDGFFIARFIKSKL"
     assembly_gap    10021..10085
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10109..11134
                     /locus_tag="DHV81_04020"
     CDS             10109..11134
                     /locus_tag="DHV81_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCI93667.1"
                     /translation="MNMDELTEFIISLGEKKFRAKQIYEWIHVKHVESFDEMTNISKK
                     FIQVLKDNAILISLKKEEVQVSKLDGTRKYLFALDDGNVIESVLMKYKHGNSVCISSQ
                     VGCRMGCRFCASTLDGLVRGLRPSEMIDQIYQIGKDIGERISNVVVMGTGEPLDNYDN
                     LLRFIELLTDENGINISQRNLTVSTCGLVPRMRQLADEKLSITLALSLHASNQEKRKA
                     LMPVANSYDIHDVVDACKYYFAQTGRRVTFEYSLVGGVNDTAEDAAELSALVHGMNCH
                     INLIPVNPIKERDYVQSNKGVIEAFKNRLEKNGINVTIRREMGRDIDGACGQLRKKHI
                     DKERGIN"
     gene            11135..11865
                     /locus_tag="DHV81_04025"
                     /pseudo
     CDS             11135..11865
                     /locus_tag="DHV81_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743169.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
     assembly_gap    11518..11613
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11882..13954
                     /gene="pknB"
                     /locus_tag="DHV81_04030"
                     /pseudo
     CDS             11882..13954
                     /gene="pknB"
                     /locus_tag="DHV81_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
     gene            14570..15478
                     /gene="rsgA"
                     /locus_tag="DHV81_04035"
     CDS             14570..15478
                     /gene="rsgA"
                     /locus_tag="DHV81_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCI93668.1"
                     /translation="MTGKIIKGIAGFYYVYVEETKEAKETATGGTLYECKAKGTFRKQ
                     KIKPLVGDIVDIAVLDDEKHIGNVERILPRKNELIRPAVSNIDMALVIFASAKPDPNF
                     NLLDRFLCMMEYQHVPVTICFNKKDLITPKEQQELKSIYEPAGYRVLFTSTKTGEGID
                     EIKHILEGRTTTVAGPSGVGKSSIINCLQDDVQMETGHISEKIERGKHTTRHSEIIPI
                     KDGTYIMDTPGFSSMDVPGFEKEDLWTCYPEFVEYEPYCRFQGCSHINEPDCGVKEAL
                     ADGKISQVRYDNYKLLYEELKNRQKY"
     gene            15426..16157
                     /locus_tag="DHV81_04040"
     CDS             15426..16157
                     /locus_tag="DHV81_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCI93669.1"
                     /translation="MIIISFYMRSLKIDKSINLKHNQTEENMNCLSPSILSADYSILG
                     EQLKQLDEAGAQYVHIDVMDGSFVPSISIGLPVIKTIRKCTERMFDVHLMIDEPVRYI
                     DDFATAGADIITVHAEACKHLDRTIEAIKEKGILAGVALNPATPLSAIEYVLPKVDMV
                     LIMTVNPGFGGQKLIPYTVDKVRDLKALLEKTANKADIEVDGGVNLENVETLLAAGAN
                     IIVAGSAVFSGDIEENVKGFLNIMM"
     gene            16234..16878
                     /locus_tag="DHV81_04045"
     CDS             16234..16878
                     /locus_tag="DHV81_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCI93670.1"
                     /translation="MRYLIVCGGKIDKEFGLNEIKTDGIDAIIAADSGMDFLYENGVT
                     PDIIVGDFDSATTKALEFFERKGQTEIHRLNPIKDDTDTEYAIRLAISEGARSIVMLG
                     ATGSRIDHVLGNISLLGIGLESKTDISIIDTNNRIRMADKPVTIEKSAQYGKFVSLIA
                     VTDDNEVSLRGFKYPVTDYSFDRFTSLGISNEIIDDHAVIDIHRGKFIIIESKD"
     gene            16985..>19405
                     /locus_tag="DHV81_04050"
     CDS             16985..>19405
                     /locus_tag="DHV81_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCI93671.1"
                     /translation="MIEKYYSGVIEQIYNRIGKETRNIVMANYSNDFSIENLEVIRRY
                     QSNEDNVFFAYSEFSYNTLVGAYEPFLDIICNMHRRFIGGSFDDFQRECGVYYLHRQV
                     LNSYYETGECFREETVLLNEVAYEQRRMTKAIVDMLKRLSEVRPLMIVINRFQMASKS
                     SIETIKYLIDNPCTNIGIVLGVNVQVKGTDSTVEVWDRIVESLEDRSAIYYIGSAGQQ
                     KNHIRDTSDDELYITTNFEQSIQEASNIMEFLDYEQAKRGCRIIEHKIKFEDAWIDEK
                     SLRRFYFIYARTSVLLGEMSKAIELTNEYKALIPESDSEHYFSIYYFMKGTCYMYQGK
                     LEKAEGCAKKAYDYALLADDELLEFKSELLSVMIKMSGWYNIFFCVQDIPVSDEFIEK
                     IIKHGYRNHLAHIYIYAYDNSRDVVKQSFYDESLLKHFTKGLELAKKIGNEQLVYDAY
                     QKTIMLASTNGLNEIAFLYVIRTYEFMKEHGNIYVARVLTSIGYNLSAMGKNELVDDY
                     YNTAINMLYYLKMPEDIAEVYYNKSLNYIMQGNYKEAVQALLIAMKTIIKLHLNSLRV
                     CNTSKVYALLALASIFSGDRFSCERYLLSCKQFLNYVIYRVIDSTRTEAVHDYSRCDD
                     EMFLYSFASAMLLWHDDKKEKAFLRFEDAERYLVNAEGNEFFAYSIYRKSRIKLFEQL
                     GKNELIEHERILLDAHNKRMHEIAEAAPLEMLKNINLESLSEGFINERQIDLLVKQQG
                     LAKDYQSSKRQIEFISIWQTIVDVNDQKKETMIENAMSNFVNHFSLDCALIIDLHAKE
                     PQVLYNDTG"
BASE COUNT         5828 a         3558 c         4926 g         4823 t
ORIGIN      
        1 atgaacatac tgcttgcata ctacaatctg atagatgact ggaaggacga taaaagcctg
       61 acgaagaaga catacgcaga gatgctgaaa aaggactttg agaaggcaaa aaaaggctat
      121 ccgatacagg ctaaggcgat agaagactac attgcaaggc ttgcagagtg cgagaagagc
      181 aatgatacca atattgatgc agttgcgggg ctgacaggag agatgctcgg catacttttt
      241 gcgtggaagc aggatgagtg gcagaaggat ctgaaggaat ttggctgcta tatgggcaaa
      301 tttatttata taatggatgc gtatgaggat attggggatg atgtgaaaaa gaagtcatat
      361 aatccactta tacagctcat gcattgctgc aataatgatc aggaaaaatt tgacaaatcc
      421 tgtcggctta tgatgacatc tatgctttca gaggcagcaa aaatatttga gaggttgcca
      481 attctcttac atgcggatat aatcagaaat gttctatact ccggtgtgtg gagcaagtat
      541 gagtatattc agatgaagaa aaggaagaaa cataaatgat agatccatat caggtgctcg
      601 gtgtcagccg cagtgcaagt gacgatgaaa taaaaaaggc ctacaggagc ttaagcagaa
      661 aatatcatcc tgatgccaat atcaataatc ctaacaagga tcaggctgag gagaagttta
      721 agcaggtgca gcaggcatat gaccagataa tgaaggaaaa gcagtccggt gctgccggag
      781 ccggtggata cggtggtgcg ggaagcagca gctcatacgg cggcagctcc tatggtggag
      841 catatggtag tggaccggga gcaggtgatc catttggagc aggtggaccg tttggtggtt
      901 tctatggccg ctatacctac aataatgcag gcagcagtgc gcaggatgac aatccaaagg
      961 tgcgtgcagc aggcaatttc atcagcaacg gctactacaa tgaggcgctg aaggtgcttg
     1021 aggaggtacc ttttgctgag agacgcggaa ggtggtacta ctacagtgct atcgcaaacc
     1081 aaaagctcgg caacacagcg acagccatag aacacataca gcgagcagtg tcgcttgagc
     1141 caaacaacat gcagtaccgc cagtttatgc agataattga aaatggtggc tcgtggtatt
     1201 cggatatggg agacatatac ggacgcccta gtggcagggg tagcgagtgc tgcagcctgc
     1261 tagccacatt cctttgctgg cagatgttct gctgctagat gtcaagcttt tctgatgtta
     1321 cagatggaaa ttcatgcaag ttattgggct ggttatttgc agaacgatgg actgttatat
     1381 taatctgtga attattctga atagttacct gaaaataaaa tgcggtcatg cgaccgcatt
     1441 ttttatttat ggacataagt aactattcag atccacacat atatgagcaa aatcagctgc
     1501 gcagcaacgg ctgtattatt ccatagaatc cggaatacca tcatgatcca aatctgccat
     1561 gtcggctccg tgcgcaccga tatccactgt gtttaaggtg cgtcttttta gtcttgcggc
     1621 ctcagactgc ttgagcagat agctctttat ctcgcgtgag tgctttacgt gaacgagctt
     1681 aagctcaggg tttgtgacgt cgcctgtgta gcagactatg gtgccgagct taaaaatgcg
     1741 ctccatcagt gatgtggtga gctttacatc agctacctta tacatgtagc agtcatcctc
     1801 tgtggtgctc aagagtccgg actccacagt gataaaggat ggtgtgatgg tgtactttgt
     1861 gaaggtccat ggcagaccga gaaacagcag tctctttctc tccttaaaga ttacggcatc
     1921 gtcatcatcg attgcgccgg ctgtggtgtt tttgatttgt gattcattta cagacatatt
     1981 attctccttt ttgatgttga aattgtcgca attgtatgaa aacttcaata caattgctaa
     2041 gcgttcagaa cagttatatt ctgtcatata ttctacaaca aaaaagtgta aaagccaact
     2101 tgaatattgc gctaagttat tatatgatag aaaaaatgta ataaaaattg ttacgcgaaa
     2161 aagaatggag gcaaattatg cgtcacttaa tgagtccgtt ggatttttct gtagaagagt
     2221 tggacaagct tctggatctt gcaaatgata tagaggctca tcctgagaaa tatgcacaca
     2281 agtgcgatgg aaagaagctt gcaacctgtt tttatgagcc aagcacgaga accaggctta
     2341 gctttgaggc tgccatgctt aatttgggag gctcggtgtt aggtttccac agtgctgatt
     2401 cgtcatctgc gtcaaagggc gagagcgttt cagatactat cagggttatt tcctgctatg
     2461 cggatatctg tgccatgcgt catcctaagg agggtgcacc gcttgtggct tcagagaaat
     2521 cgcgtatccc tgtaatcaat gcaggcgatg gaggtcatca gcatcctacc cagacacttg
     2581 ctgatctcct tacaatccgt tccatcaagg gcagattgaa taatatcaca atcggctttt
     2641 gcggagattt aaagtttgga cgtacagtgc attcactgat caatgcactt atcagatatg
     2701 acaatgtgag gattgtgctt atctcgcctg aggagcttaa ggtgcctgac tatgtgcgcg
     2761 atgatgtgct tcgtgccaac aatatcgagt tcaaggaggt agaaaagctc gaggatgcca
     2821 tgggagagct tgatgtgctc tacatgacac gtgtccagaa ggagcgtttc ttcaatgagg
     2881 aggactatgt gcgtctgaag gatttctaca ttctgacaaa ggagaagatg gagcttgcaa
     2941 aggatgatat gattgtactt catccgctcc cacgtgtgaa tgagatttct gttgatgtgg
     3001 atgatgaccc gagagcatgc tatttcaggc aggcacagta tgcggtttac gtgcgtatgg
     3061 cactgattct gacactgctt ggactggctg aggactaaag gaggataata tgctgaatat
     3121 tagtggaata catgagggct ttgtgctcga tcatatcaag gcaggtatga gtcttcagat
     3181 ttacagggat ttaaagcttg acaagctcga ctgcacagtt gcaatcataa agaatgcaaa
     3241 gagcagcaag atgggcaaga aggatatcat caaggtagag tgtccgatag agtcgttaga
     3301 cctcgatatc cttggattta tcgaccacaa tatcactgta aatgtgataa agggtgacaa
     3361 gattgtggac aagatggagc ttaagctgcc tcatcaggtg aaaaatgtca tcaagtgcaa
     3421 aaacccaaga tgcatcactt caattgagca ggagcttgac cagattttcg tgctttccga
     3481 tgagaaaaac gaaatctacc gctgcaagta ctgcgaggag aaatacgaag gagttaaata
     3541 gtatcagtta tgtatgccaa cgtgattgtg gatatatccc acgaaaaact ggataagaca
     3601 tttcagtaca gcatcccgga tgagatttta tgcgacatcc gtccgggtgt gtgcgtggat
     3661 attccatttg gcagccgcac tatcacaggc tatgtgattg aggtcacgga taagcctgag
     3721 tacgacccgg caaggacaaa gcctcttatc gggataaagg cagactctgt ggctgtggag
     3781 gcaaagctca tcgcacttgc ggcatggata cggcgcaatt acggctctac catgaatcag
     3841 gcacttaaga ctgttatacc tgtaaagaag caggctaagg agcaggtgag ccgcagtatt
     3901 gcactgaaaa tagacaaggt aaaggcaagg gcgcagcttg cgctttgcga ggcaaaaaaa
     3961 caaaaggcaa aggccaggct ttttcgtgcg ctgattgatg ctgatgatgg ggcgcgcctt
     4021 gagtattcct ttgtgacagg caagctgtcg gtgtcggctg ccactataag agcgcttgag
     4081 cagagtggct ttatcgaggt catcacacag agcggctaca gaaatcctgt ggagcatatg
     4141 agtgtggaca cctatgataa ggtactgaat gctgcgcagc agagtgtggt tgatgcgata
     4201 gaaggcgaca ttgcagctgg ccttagaaat acttatctga ttaagggtgt caccggaagc
     4261 ggcaagacag aggtgtacat ggagcttatc gcacactgta tacagtccgg gcgtcaggcg
     4321 attgtgctga ttcccgagat tgccctcact taccagacag tgatgcgttt ttttgccaga
     4381 tttgggaaca gggtttcgat aatcaattca agactttcaa atggtgagcg ttatgaccag
     4441 tttgagagag ctaagaatgg tgatattgac atcatgattg gcccgcggtc ggcgctgttc
     4501 acaccttttt caaatttagg acttatcata atagatgaag agcacgagaa ttcatacaag
     4561 agcgagtcgg tgccgcgcta tcatgccagg gaggtggcga tagagtacgc gcgcatgtcc
     4621 gatgccattg tggtacttgg ctctgcaaca ccgtcggtgg atagctatta caaggcaaag
     4681 acaggtgttt acaggctttt ggagctggat aagcgtgtgg atgacagacc tcttccaaag
     4741 tgtgaggttg tcgatttgag gcaggagctt cgtgagggaa accgctcgat tctttccacc
     4801 aggcttcagg agctcatgga ggagaggctt ctaaatggcc agcagaccat gcttttttta
     4861 aatagaaggg gcaaatcggc atttatgtcg tgcagagcgt gcggttttgt ggtaaaatgt
     4921 cctcattgcg atgtatcact cagtgaacat agcggcggag tgatggtctg tcactactgc
     4981 ggctacagac agccggtgcc taaggtgtgt ccgagctgtg gctcgaagta cattagcggc
     5041 tttaaggccg gcacacagaa gatagaagcg atggtggcaa agcgttttcc acaggcgcgt
     5101 atactgcgca tggattatga caccacgagg gcgaaggatg cgtatgagaa gattttgcag
     5161 gcattttcca atcatgaggc cgatatactt atcggtacgc agatgattgt aaaggggcat
     5221 gatttttcaa atgtcacgct ggttggtgtg ctggcggcgg atatgtcact gcatgtgaat
     5281 gattttcatg cggcggagcg cacctttgct ctgctgaccc aggcggcagg gcgcgccggc
     5341 agaggaaagc tgcccggcaa tgtggttatc cagacctacg accctgacca ctatgccatc
     5401 cggactgcca aagagcagga ttatgaggct ttttacgata aggagataga gtacaggagg
     5461 cttatgaact atccacctgt atggaatatg cttttagtcc atgtcacatc acctgatgag
     5521 agtgagtgcg gcagcatggc acagcaagtg tacgatatag cgtcgcagat gatatcgcat
     5581 acagatgaaa atcagagccc ggatgatagg catctgattc agctcatcgg tcctgcggat
     5641 gcgaccatcg ccaaggtaaa tgacatatac cgcaaggtga tttacatgaa gacatctgac
     5701 tatgcggcgc ttatttccat aaaggatggc atagagcagg ctgtgaaagc tgatacggca
     5761 atgaaacatg ctaatatctc atttgatttc aatccgatga gtggattttg atagatatag
     5821 aaggaggcaa ttatggcact tagaactatt agagtggagg gagaccctgt acttggcaag
     5881 gtctgcagag aggttacgga ggtgactcca aagatagtaa cacttattga tgatatgctt
     5941 gagactatgt atgaggctaa tggtgtagga cttgccgcac cacaggttgg catattaaag
     6001 aggatcgttg ttatcgatgt gggcgagggt ccaatcgtga tgattaatcc tgaaattatc
     6061 gagtctgacg gagagcagac cggcgatgag ggctgtcttt cagtaccggg aaaggccgga
     6121 caggtgaccc gcccgaatta tgtaaaggct cgtttcatgg gcgaggatat gaatgagtat
     6181 gagatagagg gagaagagct tttggccaga tgtatctgcc acgagcttga ccatctcgac
     6241 ggacatctct atgtggagaa ggttgagggc gcacttcacg atgtgacata tgaggataca
     6301 caggagtaat cattaggatg acatttgtta tctgagcttg gaaaatatct gggaggcagt
     6361 aagatgagag taattttcat gggaacaccg gacttttcgg tgggaacnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     6481 aatgcagtat acaccggtca aggagtgtgc gctttcgcac ggtattgagg tattccagcc
     6541 ggttaaaata agagagactg ctaatataga gtatctcaga aagtttaatg cagatataat
     6601 aattgtagtt gcttttggac agattctgtc aaagagtatt cttgatatgc ctcgatacgg
     6661 ctgtatcaat gtgcatgctt cacttttgcc aaagtacaga ggtgcggctc ctatccagtg
     6721 ggctgtgatt aacggtgacg agtttacagg tgtcactacc atgaggatgg atgagggtgt
     6781 cgataccggc gatatgattg caaagagtac agtcagactt gcacctgatg agaccggcgg
     6841 cagcctgttt gataagctct cagcagaggg agcaaggctg tgtgtggaga ccatgaagat
     6901 gatagaggat ggcactgcag agtatactcc tcaaaacagc gaggaggcta cacatacatc
     6961 aatgatcagt aaggagcttg gctttatcga ctggacaaag ccggctgttg agattgagag
     7021 gcttataagg gggcttaatc catggccgag cgcatacacc cacttaaacg gtaagacatt
     7081 taaggtgtgg agtgcaaagg tgattgacgg cagcgatgat tatgagccgg gctgtattta
     7141 tcatatcgga aaaaatgata tgtatgtaca gacaggaaaa ggagcattat ctttagtaga
     7201 ggtacagctc cagggcaaga agcgcatgga tacaggttca tttttgagag gctgtcatgt
     7261 ggaagagggc tcatttttca cgttatagtt attcattata agtaactaaa aacataatta
     7321 aatgtgagtc aatgttctca aagatactca aaatccagag tattcgtttg tgctaacgtc
     7381 aaagcagtcg gaaacgttga gaagtagcca gtgcaagttg aaacagtctt gctaattata
     7441 ggatagaacc ttgatggtag cgtttagaca atggtagaag cctacgaaaa ccaagtagcg
     7501 aaacataaaa tattaagaaa ggaaaagcta tttagatgaa aacctatatc taatcaattt
     7561 aaagaattta taggtatgta ccgatggctt agtcgggaat ttacgactgt ggagtgtaca
     7621 agaacttgtg agtagcgtat tgtttataat cgccaaagca tacacattga agcagtaact
     7681 acaaatcgtg agattgtagc gaatcatcta gcatatacac atttgtatat gtttttagta
     7741 gcagagatga tacatgggat attattactg ggatccgaca tatatactcg ttgtgatagg
     7801 tgcagtgata tgtatgattg catcggcaag agttaaaggt acatttaata aatactcaca
     7861 gctaagaagc atgtcaggca tgaatggtgc gcaggtggct cagagagtgc ttcaggcggc
     7921 aggcatatat gatgtgcagg tgcgtcatgt gtctggcagt ctgacggacc actatgatcc
     7981 gcgcaccaaa acagtgaatt tatctgatcc tgtgtacaat gccacatcgg tcgcagcact
     8041 tggcgtagca gcccacgagt gtggacacgc tatacagcat gcaaaaagct atgcacctct
     8101 ttcaatcagg agtgcgctcg tacctatagc caattttggc tccatgcttg catggccggt
     8161 tatacttatc ggcttattat ttaacacacg ctcgtcaggg cttattattg atataggcat
     8221 attgctgttt tcggcagcag tgcttttcca gctagtgaca ttgccggtag agtttgatgc
     8281 ttcaagacgt gcgcttgtca tgcttcgtac acagggcata ctttcagatg acgagcttaa
     8341 gtacacaaga aaggtgctca agtcagcggc acttannnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnttacagct ccttcgtata atactcatca caaacggacg cagacgggac
     8461 gactgatatt gttttagctg ctgtttgtct gtgattttac agggtggcag ttcaaattgg
     8521 atatattagt tagaaaatta aatgtatatg taactattcg gaaacatcat catgataaat
     8581 aatcatagta aaaataatca taataataaa aatagccaaa taaacaaaaa taatataccg
     8641 ggcagaccgg caaaatcaaa cgtggcagcg cataatgagc attctgtaga tacgcgtgag
     8701 cttgcccttg agatgcttat tgagattagt gagcgtggag cattttcaca tatcgtgctt
     8761 cgcagtgtgc ttgacaagta tcagtatctg tcaaagcagg acagagcctt tatgacgagg
     8821 cttgttgacg gcactatcga gtatatgctg cagcttgact acatcataga ctctttttca
     8881 aagacacaga taaggaagat gaagcccttc atcagaaacc ttcttcgcat gagcgtctac
     8941 cagataaagt acatggacgc ggtgccggat tcagctgtat gcaatgaggc ggtgaagctt
     9001 gcaaagaggc ataagtttgc acagctttcg ggctttgtaa acggtgttct cagaaatatt
     9061 gcaagaaata tagattcagt acagtttgat accttaagca tcagatactc catgccacag
     9121 tggatagtgg acagatttgt atcagcatac ggagagaaga aggcagagga gattttcaag
     9181 gcctttcaca ataagagcac aataagcatc aggacaaacc tgaccaggtg tactccggat
     9241 gagcttcgtg caatgcttga ggcagagggt gtgacggtca cagcggttga tgaacttaat
     9301 tatgcgtttg ttatatctgg atttgattat ttaaatggct tgcagagctt tagagatggg
     9361 cttttctatg tgcaggatat cagctccatg cttgtcgccc agacagctgc tccaaagaag
     9421 ggcgactatg tgatagatgt atgcgcagca ccgggtggca agagcaccca tatagctgag
     9481 cttttacagg gaagtggcca tgtgctcgcg cgtgacctga ccgataccaa ggtcgatatg
     9541 attgaggaaa atatagaaag gcatggcctt aacaatatgt cggcagaggt gtgggatgcc
     9601 acagtgcctg atgctgattc tgcgggaaag gcagatatac tcatctgtga tctgccgtgc
     9661 tcagggcttg gagtgctcgg cagaaagaag gacatccgct acaagatgac acctgagtct
     9721 gtagatgagc ttgccgctct tcaaagacag atacttgata ccgtgcatac atacgtgaag
     9781 acaaacggag tgctcgtgta cagtacatgt acgatagatg aggttgagaa tgaggacaat
     9841 gtgaggtggt ttattgaaaa gcatccggag tttgagctgg ataagagctt tgcggaaggc
     9901 actggaatga aacagatact gcccggtgag catggaagtg acggattctt tattgcaaga
     9961 ttcataaaaa gcaaattatg atattaaaat aaatcatgaa ttaataaata taaattggtt
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnaacaa ctaactgaca tcaaatcaat gaatatggac gagctcacag agtttatcat
    10141 atcgctcgga gagaagaaat tccgtgcgaa gcagatatat gagtggattc atgtgaagca
    10201 tgtggaaagc tttgatgaga tgaccaatat ttcaaaaaag ttcatacagg tattaaagga
    10261 caatgcaata ctcatctcgc taaaaaaaga ggaggtgcag gtgtcaaagc tcgatggcac
    10321 gagaaaatat ctctttgcac tcgacgatgg caatgtcata gaaagtgtgc ttatgaagta
    10381 caagcatgga aactctgtat gcatatcttc gcaggttggc tgcaggatgg ggtgcagatt
    10441 ctgtgcatcc actcttgatg gtctcgtcag ggggcttagg ccgtctgaga tgattgacca
    10501 gatttatcag ataggcaaag acataggaga gcgcatctca aatgtagttg tcatggggac
    10561 aggtgagccg cttgacaatt acgacaatct gctgcgcttt atagagcttc tcactgatga
    10621 aaacggtatc aatatcagcc agcgcaatct gacagtttcc acatgtggtc tcgtaccgcg
    10681 catgagacag cttgcagatg aaaagctttc tatcacgctg gcgctttcac tgcatgcctc
    10741 aaaccaggaa aagagaaaag ctctcatgcc ggttgcaaac agctatgata tacatgatgt
    10801 ggtcgatgca tgcaaatatt attttgcaca gaccggccgc agagtcacct ttgagtatag
    10861 cctggtaggc ggagtgaacg atacagccga ggatgcagca gagctttcgg cactcgtaca
    10921 tggcatgaac tgccatataa atctgattcc ggtaaacccg ataaaggagc gcgattacgt
    10981 gcagtccaac aagggtgtta tagaggcttt taaaaatagg cttgaaaaaa acggaattaa
    11041 tgttactatt agaagggaaa tgggcagaga tatagacggt gcctgtggac agcttcgcaa
    11101 gaagcatata gataaagaga gaggaataaa ctagatgaag acgttttcca ttacggatgc
    11161 aggtgctatt cgtgagatga atcaggattt ttatttctcg tctgacactg cagtaggaaa
    11221 tcttccgaat cttttcatcg tagcagatgg aatgggagga cacaaagccg gtgactatgc
    11281 ttccagatac actatagagc gtgtggttgc atcggtatcg agaaatgccg gggatgaacc
    11341 ggtatccatc atcaaagagg ctatcaacaa ggcaaatgag cttttggtgg ctgagtctcg
    11401 tgaggatgaa gctaagcgtg gaatgggtac caccttagtc ataggaacta ttataggaaa
    11461 caagcttttt gtggccaata taggtgacag caggctttat attgtaggtg gcacagannn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagaata tcatcacgag agctgtcggg
    11641 gcagcagatc atgtggaggc agatttcttt gaggtcgagc tatgtgagga cgacaggatt
    11701 cttctgtgta cagacggact taccaatatg gtgagagatg aagaaatatg cgatactatt
    11761 aggcaaaatg ataatatcga gacagcagcg tcacagcttg tcgccatggc caatgcaaac
    11821 ggtggccgcg ataatatcac tgtaatgatt attaaaccat tttaagacga ggtaataaca
    11881 gatgttagag ataggaagtt ttttatcgga tcgatatgag atcctaagca aggttggcgc
    11941 aggcggcatg tccgatgtat acaaggcaaa agaccatata ctgagccgtt tcgtagctat
    12001 aaaggtttta aagcaggagt tttcggagga tagttcattt gtgaccaagt tccgtgctga
    12061 ggcacagtca gcagcaggac ttgagcatcc gaatatcgta aatatctatg atgtaggcag
    12121 cgagaatggt ctgtactaca ttgtcatgga gtatgtcgag ggcatcacac ttaagactta
    12181 tatcgagaaa aagggccagc tgtcattcaa ggagtctgca agtattgcaa tccaggtggc
    12241 aaggggcata gaggccgctc acaataaaaa tatcatccac cgtgatatca agccgcagaa
    12301 tatcattatt tctacagatg gcaaggtaaa ggtgacagac tttggaattg caaaggctac
    12361 atcttcaaat acaataagct cagatgtgat gggctcagta cactatgcat ctccggagca
    12421 ggcaagaaac ggcttcgtcg atggcagaag tgatatttac tcactgggaa tcgtaatgtt
    12481 tgagatggtt acaggccgtg tgccgtttga cggtgatacc acagtggcag ttgcactgca
    12541 gcacttacag gaggagattg caaggccaag catctacgca ccggatctgc caatcagctt
    12601 tgagaaaatc atcttaaagt gtacacaaaa gacaccggac cgcagatacc agactattga
    12661 ggagcttctc acagacataa ggcgctctct tgcgcatcct gatgaggatt tcgtcacaat
    12721 agcaccactc gttgacggtg gcaagaccaa ggttataagc ccggaggagc ttgacaagat
    12781 aaaagagggc agaggtgtcg cagaggactt aaacgatgat gataccgatg cagataatga
    12841 tgatgagtat gctgacgatg aagacgatga tgatgaatat gatgagtcac tcttagacga
    12901 cgatgatgac gaagaagacg atgacgatga tgatgacggc aagcttttaa atccaaagat
    12961 ggataaagca atcacaatca tgggtatagt aacagccgta atcattgtaa tagttatcat
    13021 atatcttgca ctttcagtgg caggagtgtt taagtttggc ggcaaaaaga actcagagtc
    13081 acagcagaca gagtcacaga cacagaccga gtctgaatca gagtcagaga cacagactga
    13141 gacagagggc cagatgattg acatcagagg catgtcagtg gaggatgctc agaaggcagt
    13201 agatcgctta aagcttgacc tcacagtatt tgcatttgag acaaagcagt cggataaaaa
    13261 ggacggcaca atccttgagc aggatgtcaa ggccggagat acagtaaaac gtggctcaca
    13321 gattaatgta gttatcgcag gaaaaggtga tagcacatct gagatggtca aggtaccaag
    13381 tgtaatcgga aagaccaaga gctcggcaaa gagtacactc gaatcagcag gctttagtgt
    13441 caccttcgag tatggagatt acaatgacag tgtagcagca gatgtagtaa cagcacagtc
    13501 accatcagca aagaaacagg ccgcaaaggg cagcacagta acagtcaccc tctcaccggg
    13561 ccagaagcca atcaccgtgc caaacgtagt aggcgcctca cagtcacatg cagaaagcgc
    13621 tctcgcagga gcagggctca agtacaccta cgccgactcc cagtacagcg acacagtacc
    13681 cgccggcagc gtaatcagcc agaccaaatc cggcgaaacc gtagccgcag gcactacaat
    13741 cacactgaca ctcagtaagg gtaagcagga gatatcaact aacgtaagta agacagttaa
    13801 gcttgatatc ggagaagtta atattacagg tggttcatat tcacttgtgg gttctgacgg
    13861 aaaaacttat gcaagcggaa gtgatgtaac atcagcttca gttacagtaa gtggaacaat
    13921 gaactgtaaa acaggtacag taacggttac atggcagtat acagagcctg tcaaggatga
    13981 gaatggaaat gtaacaggtg aaaagccagg agaaaaaacg atttctgtac aagtaccata
    14041 atttgtatat tgtaaaataa aatcataatt tgatgaaaac gtggatggca atacaaggaa
    14101 agcgggagta ttcccagtac aaggaaaacg agccgtagcg cagctacgtc aagttttccg
    14161 taccgccatc atatttgtaa atacacacga tacaaatata gcgcataaag aagcaaccca
    14221 atacacgctt catagtaagc caccggatag cacccacgaa gtggtgcaat ccgtacccca
    14281 ctaccacacc aacccgcgga cgaaaacctg aactcgcgga gaaggactgt agatagtggg
    14341 gcaggcatgt gttccgagga aaaaatatga actgccccgg ggtgccataa acctaccgag
    14401 gaaagaacat ggcagagcaa accatagctt gtctgtagaa gagcccgggg tttgcttcat
    14461 attttttaag aggaatatat gcatgccacg ccacacacac aacctcaaag cgcggcatcc
    14521 ccaccgaaag cgtgactcct gcaaatacaa aagcaataca aggaaaacca tgacaggaaa
    14581 aattattaaa ggaattgccg gcttctacta tgtctacgta gaagaaacca aagaagcaaa
    14641 agaaactgcc acaggcggca ctctctacga gtgcaaagca aaaggcacct tcagaaaaca
    14701 aaagataaag ccgctggttg gcgatatcgt cgacatagca gtcctcgatg atgaaaagca
    14761 cataggcaat gtcgaacgta tactgccacg gaaaaacgaa cttatcaggc cggctgtgtc
    14821 caatatagac atggccctgg ttatttttgc atccgcaaag ccggatccca atttcaacct
    14881 gcttgatagg tttttgtgta tgatggagta ccagcatgtc ccggttacca tatgcttcaa
    14941 caaaaaggac ttaatcacac ctaaagagca gcaggagctt aaaagcatat acgagccggc
    15001 tggctataga gtgcttttta ccagcacgaa aacaggtgag ggcatagatg agataaagca
    15061 cattttagag ggaaggacga ccacggttgc aggtccctcg ggagttggca agtcatctat
    15121 cattaactgt ctgcaggatg atgtgcagat ggagaccgga catataagtg agaaaataga
    15181 gcgcggaaag catacgacca gacattctga gattatccct ataaaggatg gcacgtatat
    15241 catggataca ccgggcttta gctcgatgga tgtgcctggc tttgaaaagg aagatctgtg
    15301 gacatgttat ccggagtttg tagagtatga gccatattgc aggtttcagg gctgcagtca
    15361 tataaatgag ccggactgcg gtgtgaagga agctcttgca gatgggaaaa tatcgcaggt
    15421 gcggtatgat aattataagc ttttatatga ggagcttaaa aatagacaaa agtattaatt
    15481 taaagcataa tcaaacggag gaaaacatga actgtttatc accatcaatt ttatcagcgg
    15541 attattcaat actcggagag cagcttaagc agcttgatga ggcaggagca cagtatgtgc
    15601 atatagatgt tatggacggc agcttcgttc caagcatatc aataggactg ccggtcataa
    15661 agacaatcag aaagtgtaca gagcgtatgt ttgatgtgca tctgatgata gatgagccgg
    15721 tcagatatat agatgatttt gcgacagcag gagcagatat catcacagtc catgcggagg
    15781 catgtaagca tcttgacagg acaatcgagg caataaagga aaagggcata ctggcaggtg
    15841 tagcattaaa tccggctact ccgctctcag ccatagagta tgtgcttcca aaggtggaca
    15901 tggtgcttat catgactgta aatccgggct ttggaggaca aaagcttatt ccgtacactg
    15961 tagacaaggt gcgcgactta aaggcgcttc ttgagaaaac cgcaaataag gctgatatcg
    16021 aggtggacgg tggtgttaat cttgagaacg tggagacact gcttgcggca ggagccaata
    16081 tcattgttgc gggaagtgcg gtgtttagcg gagatataga agaaaatgta aagggttttc
    16141 tgaatatcat gatgtaacag atgcaggtta aaaccaaaat gcttccgggg ctgtagcaaa
    16201 tgtggatatg ttatttataa gaaggtggat aaaatgaggt atttgattgt atgtggtgga
    16261 aaaatagata aggaattcgg cttaaatgag ataaagactg atggaataga tgccataatt
    16321 gcggctgaca gcggaatgga tttcctgtac gaaaacggtg tcacgcctga tataattgta
    16381 ggtgatttcg attcagccac aacaaaggcc ttggagtttt ttgagagaaa gggacagacc
    16441 gagatacaca ggctcaatcc cataaaggat gatactgata cagagtatgc cataagactt
    16501 gccataagcg agggtgcacg aagcatagtg atgcttggag caacaggaag cagaatagac
    16561 catgtgctcg gcaatatttc actcttggga ataggattgg aaagtaaaac tgatatcagc
    16621 attatcgaca caaacaaccg tataaggatg gcggataagc ccgtaacaat tgaaaaatca
    16681 gcccagtatg gcaagtttgt ctcgcttata gcagtgacag acgacaatga ggtgagcctt
    16741 agaggcttta agtatcctgt tacggattat tcctttgaca gatttacatc gcttggcata
    16801 tccaatgaaa taattgatga ccatgcagtg attgatattc atagaggaaa atttatcatt
    16861 attgagtcaa aagattgact ttttaggaat tttgggctat tcttacttta aggattattt
    16921 cggaaattta actacatgga aagatttatc ctgtgtcaga tttagaacgt gatggggtta
    16981 tcagatgata gaaaagtatt atagcggggt aattgaacag atttacaatc gcataggaaa
    17041 agagactcga aatatcgtaa tggcgaatta tagcaatgat ttttcgattg aaaatcttga
    17101 ggttataaga cgttatcaaa gcaatgagga taacgtcttt tttgcataca gtgagttctc
    17161 ttacaacact ttagtcggag cttatgagcc atttttagac atcatatgca atatgcacag
    17221 gcgctttata ggcggaagct ttgatgattt ccaaagggag tgcggtgtgt actacctgca
    17281 cagacaggtg ttaaacagct attatgagac cggtgagtgt ttcagagagg agacagtgct
    17341 gttaaatgag gtagcatacg agcagagacg catgactaag gccatagttg acatgcttaa
    17401 gaggctttca gaagtgaggc cattgatgat tgtgataaac agattccaga tggcgtcaaa
    17461 gagctctatt gagaccatca agtatctgat tgacaatccg tgcaccaata tcggtattgt
    17521 gttaggtgtt aatgtccagg tcaagggtac tgacagtact gttgaggtgt gggatcgtat
    17581 agtcgagtct cttgaggaca ggagtgccat atactatata ggcagtgccg gacaacagaa
    17641 gaatcatata agagacacaa gtgatgacga gctgtatatt actacaaact ttgagcagag
    17701 tatccaggaa gcatcaaata ttatggagtt tctggattat gagcaggcaa agagaggctg
    17761 tcgtatcata gagcacaaga ttaagtttga ggatgcatgg attgatgaaa agagtctgag
    17821 acgtttttat tttatctatg caaggacatc tgttttactt ggagagatgt ccaaagcgat
    17881 tgagcttacg aatgagtaca aggcactgat accggaaagt gacagtgagc attatttttc
    17941 tatatattat tttatgaagg gcacctgcta tatgtatcag ggcaagctcg agaaggctga
    18001 gggctgtgca aagaaagcct atgactatgc acttttggcg gatgatgaat tgctcgaatt
    18061 caagtccgag cttttgtccg ttatgataaa gatgtcgggc tggtacaata tatttttctg
    18121 tgtgcaggat atacctgtca gtgatgaatt tatagaaaag attataaagc acggatacag
    18181 aaatcatctt gcgcatatat atatttacgc atatgacaat agccgggatg tggtgaaaca
    18241 gtcattttat gatgaatcat tgctaaagca ttttacaaag ggacttgagc ttgcaaagaa
    18301 gataggaaat gagcagcttg tgtatgatgc atatcagaag actatcatgc ttgccagcac
    18361 aaacggtctc aatgagatag catttttata tgttatccgt acatacgagt tcatgaagga
    18421 acacggcaat atatatgttg cgagagtact tacgtctata ggctacaatc tgagtgccat
    18481 gggtaaaaat gagcttgttg acgattatta taatactgca ataaatatgt tatattatct
    18541 gaagatgccg gaggatatag ctgaggtgta ttataacaag tctcttaact atattatgca
    18601 gggcaattat aaggaggctg tccaggcact tttaattgcc atgaagacta ttataaagct
    18661 tcatctaaac agcttaagag tgtgtaacac gagtaaggta tatgcgcttc ttgcacttgc
    18721 atctatattt tcaggtgaca gattcagctg tgagcgctat ctgctcagct gcaagcagtt
    18781 tctcaattac gtcatataca gggtaattga cagcacaaga acagaggctg ttcatgacta
    18841 ttccagatgc gatgatgaga tgtttctgta ctcatttgca tcggctatgc tcttgtggca
    18901 cgatgataaa aaggaaaaag cctttttacg gtttgaggat gcagagagat acctcgttaa
    18961 tgcggagggc aatgagtttt ttgcatattc gatatacagg aaaagcagga ttaagctgtt
    19021 tgaacagctc ggcaaaaatg agcttataga gcatgagaga atacttttag atgcacacaa
    19081 taaaagaatg catgaaatag cagaagcagc tccgcttgaa atgttaaaaa atatcaactt
    19141 agagtcactg agtgaaggct ttatcaatga aagacagatt gatctgcttg taaagcagca
    19201 gggacttgct aaggactatc agagctcaaa gcgccagatt gagtttattt ccatatggca
    19261 gactattgtc gatgtaaatg atcagaagaa ggagactatg attgaaaatg ccatgagcaa
    19321 tttcgtaaat catttcagtc ttgactgtgc gctgattata gatctgcacg cgaaggagcc
    19381 gcaggtgctt tataatgata cgggc
//
LOCUS       DPUF01000109            7415 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12, whole
            genome shotgun sequence.
ACCESSION   DPUF01000109 DPUF01000000
VERSION     DPUF01000109.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(53..1609)
                     /locus_tag="DHV81_04055"
                     /pseudo
     CDS             complement(53..1609)
                     /locus_tag="DHV81_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517088.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
     assembly_gap    1046..1172
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1659..2828)
                     /locus_tag="DHV81_04060"
     CDS             complement(1659..2828)
                     /locus_tag="DHV81_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCI93672.1"
                     /translation="MIQQNVLDKFKELFGADGDIRVYFAPGRVNLIGEHTDYNGGHVF
                     PCALTIGTYMAARKRDDRKLRFYSMNFDNLGVVESSLDEFTPDPDGLWINYPMGVMWA
                     FEGRGMKLESGLDIVLFGNIPNGSGLSSSASLEVVTGYMLKDIYGFDVTNQDIALIGQ
                     YSENNYNGCNCGIMDQFASAMGKKDNAIFLDCNTLDFEYAPIVLDGAKIVVTNSMVKH
                     SLVTSAYNDRRNESAQALKDLQTVCDIKTLGDLTDEEFEAHKDAIQDEVARKRGKHAV
                     YENQRTIKAVKALKENDIETFGKLMNASHVSLRDDYETSCPEVDVLVDEAWKIPGVIG
                     SRITGGGFGGCTVSIVKDEAIDQFKANLTKAYEEKVGKTPEFYVVSIGDGPSRLA"
     gene            complement(2998..4107)
                     /locus_tag="DHV81_04065"
     CDS             complement(2998..4107)
                     /locus_tag="DHV81_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCI93673.1"
                     /translation="MSKFIKGMDLSTLLELERCGAKYYEDGKEKDILDIMKEHDVDTI
                     RLRLWNDPKSEEGEPYGAGNNDLAETIAIGKKVTDAGFGVLLNFHYSDFWADPGKQIK
                     PKAWKNFGADELEQAVYDFTLENLTKIIEAGVNVTMIQVGNELSNGLLWPEGKVPNYD
                     NIAKFVNAGIRACRKVNADIPIMIHLDNGGNNELYVRWFTNFIERGEEFEYIGLSYYP
                     FWHGSLDQLEFNMNDIAKRFNKDLIIAEVSMGFTMDSYQEYEKLADSERKGYATKPEL
                     VEKIDYPMTIEGQADFTKDFLNRVANVVDDHGKGFFWWEPAWIPVPGSGWATPASLKY
                     MNDPGPCGNEWANQALFDYDGNVLPAFDVIKCFKK"
     gene            complement(4230..7313)
                     /locus_tag="DHV81_04070"
     CDS             complement(4230..7313)
                     /locus_tag="DHV81_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCI93674.1"
                     /translation="MSNFDSNIIKNPEIFEQNRLAAHSDHVCYKNELEKIKGKSSLRY
                     DMNGLWKFAYAKNQSLAPCGFEAADYDCKGWDEIRVPAHIQMEGYDVPIYTNTTYPWE
                     ADESIKPGEVPEIFNPVASYVKYFTIPENMKNKRVCISFQGVESGFALWLNGHYVGYS
                     EDTFDPSDFELTDYIVEGENKLAVRVWKWTSSSWCEDQDFYRFSGIFRDVFLYAVPCA
                     HVEDLSVVPTLNDTFDEGTLSVSIKADGDGIASVKLYELGDLSVEKYDRAKLLLEEFD
                     IELRNKEICEGSCNVKNPLLWSAEKPNLYEVKIIVKDTHGNETEFISQLAGFRRFEMV
                     DGLMKLNGKRIVFKGVNRHEFSSITGRVPNRDEVIKDIVTMKKNNINAIRTSHYPDDS
                     MLYKLCDIYGIYMIAENNLESHGTWEAYNKGYVDLDFVVPKDKPQWREMMLDRANSCY
                     QRDKNHPAILIWSCGNESFGGKTIYEMSQLFRQLDKHRLVHYEGVFNDRSYNDTSDME
                     SQMYTPAAGIEKFLAEHPEKPFICCEYTHAMGNSCGAMHKYTELSDREPRYQGGFIWD
                     YIDQSIYKKDRYGKWFLTYGGDFGDRPTDGDFSGNGICYGGEREASPKMQEVKFNYQN
                     ISVDFDSDYIFTVTNKNLFVNTSVFDAFAILLADGEEVYRTKLQISVPPMDRASYEVP
                     VTLKNSMIDVEKEYCIVVSFVLKENTIWEKAGYEIAFGQHMIKKPVSEYSCDKSVELV
                     VGNGNILVRGENFKALFSRMNLGMVSYVYGGVEMLPNTIPLPNFWRTPTNNDSGNMMP
                     QRYAQWKIASMYVTTRQDQRFADTSPRVEKNDNNIAITYTYFMPTTPQSSCEVTYRVF
                     GDGTIETTLSYDPVKELGDMPEFGMMFKLDADYDTVKWYGLGPQETYEDRQHGGKYGV
                     YENKVADNVAEYLVPQESGNKCRVRYAKVMDKKGRGMLFFGDELSFSALPYTPHELEN
                     AAHHFELPPVHYTVVRVAKKQMGVGGDDSWGAHTHPEYLLDASEKMEFTFCFRGI"
BASE COUNT         1920 a         1834 c         1278 g         2256 t
ORIGIN      
        1 tatagtatgg ttcgccggtg ctttccgcat tataaaaaga taattgaaaa tcttatgcat
       61 ttaccgcatc tataaatttt tggaacgctg cacgtccctc atcagtgcac ttgtacactc
      121 ctgcacactc aagcacttct ttaaatacaa gtccaatctc ctcattgatg atatcatcaa
      181 tatttgactc atcaatctta tcatacttag acataaactt aagagcccag tcagcatgtg
      241 atgcaagagt ctccgtcgaa tgcagatcct ttcctgtaag gattgcatcc ctaagctctg
      301 ccatctcacc ttttaatctg gccggaagca ctgcaagtcc cattacctcg ataagtccga
      361 tattctcctt ctttatgtga tggaggtggg catgcggatg atatacgcca agcggatgct
      421 cctctgttgt gatgttgttt ctgagcacaa ggtcaagctc atagtctccg tcacgacgtc
      481 ttgctatagg tgtgatagtg ttgtgtggct ctccatctgt ctctgcaaaa ataaatgctt
      541 cttcatctgt atagcctctc catgcaagaa gaatcttatc tgcaagatca ataagcttat
      601 ccctgtctgc cgacttgatt ctgataacag acataggcca ttttacgata cctgcttcaa
      661 catcctcgta gcccttgaat gtgatttttt tctctatcgg agcctttgcc atagcgaagg
      721 tatagtgtcc gccctggaaa tggtcgtgac ttaagataga gcctccaacg attggcagat
      781 ctgcatttga gcctacaaaa taatgtggaa actgtgttac aaaatcaagc agctttccaa
      841 atgtggcacg ctcgattttc atcggtgtat gctttgaatt gaacacgata cagtgctcat
      901 tgtagtatac atatggtgag tactggaaaa accactggct gttgtttatt gtaacaggga
      961 taattctgtg gttttctctt gcaggatggt ttacgcggcc tgcatagccc tcattttcct
     1021 tacaaagctg gcactttgga tatgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgctgtct tctgatataa ttgctgtcac
     1201 agcttaattt gtaaaaataa tcggttgccg caagtgaaga ctcattgtcg tataaatcct
     1261 taaagcgagc tctgatcacg cttggtgcat ttacaaggca gcccatcagc tttgtatcaa
     1321 aaagatcctt gtatacaata ctgttttctt tcatgatgcc attgtcatat gcgtatgtca
     1381 tcatatcctc gagtataccc tcaagcttct cctctgcctc ttcctgtgtc catgcaagct
     1441 gagcctctac cttttcaaat gtctcatcct caatatcgtc cactccgagt acctcgagaa
     1501 gtctgtttac tgtgtatacc tcatcagcag ggtcaacaag tcctgttgta agaccatatt
     1561 ttactaattc taatattctc tcctgaatca tgtcatgctc cttaatcatt gtcgggaaaa
     1621 acctgtttat ccgttactgt tatttcatat tgctaatttt atgccagacg gctaggtccg
     1681 tctccgattg aaacaacata aaactcaggt gtctttccaa ccttctcttc ataggccttt
     1741 gtcaggtttg ccttaaactg atcaatagcc tcgtccttta ctatgctgac tgtgcagcct
     1801 ccaaagccgc cgcctgtgat tcgtgatccg ataacacccg gaatcttcca tgcctcatca
     1861 acaagtacgt caacctcagg acatgatgtc tcataatcat cacgaagtga tacatgtgaa
     1921 gcattcataa gctttccaaa tgtctcgata tcattttcct tcagtgcctt aacagccttg
     1981 atggtacgct ggttctcata aaccgcatgc ttgcctctct tacgtgcaac ctcatcctga
     2041 atcgcatcct tatgagcctc gaactcttca tctgtcaggt caccgagagt cttgatgtca
     2101 catactgtct gtaaatcctt aagagcctgt gcactctcat ttcttctgtc attgtaagca
     2161 cttgttacaa ggctgtgctt aaccatgctg tttgtaacca cgatctttgc accgtccaaa
     2221 acgattggtg catactcaaa atcaagagta ttgcagtcaa ggaaaattgc attgtccttt
     2281 ttgcccattg cagatgcaaa ctgatccatg ataccgcagt tacagccgtt gtagttgttc
     2341 tctgaatact gaccgataag tgcaatatcc tgatttgtca cgtcaaagcc gtaaatatcc
     2401 tttaacatat atcctgtaac cacctcaaga gatgctgatg aagaaagtcc tgagccgttt
     2461 ggaatatttc cgaataaaac aatatcaaga ccgctttcaa gcttcattcc gcgtccctcg
     2521 aaggcccaca taacgcccat tggatagttt atccaaagtc cgtccggatc aggtgtaaac
     2581 tcatcaagtg atgactcaac cacacccaga ttgtcaaaat tcatagagta gaaacgaagc
     2641 ttcctgtcat cgcgctttct tgctgccata tatgtgccga tggtcagtgc acaagggaac
     2701 acatgtcctc cattgtagtc agtgtgctca ccgatcagat ttacacgtcc cggagcaaaa
     2761 tatactctga tatccccatc tgctccaaaa agctccttga atttgtctaa tacgttctgc
     2821 tgtatcattt tttcttctcc tttgctgatt gtatttgcgt taaattgata gttaaaatat
     2881 ttcgtttttt ctttataatt ctattataac tgcacatttt tacattgcaa tacatccgac
     2941 tgaaaatgca acataacaaa tcaaatcatg caacaaaatt acatatattc tttgtaatta
     3001 ttttttgaaa cacttaataa catcaaatgc cggcaacaca tttccatcat agtcaaagag
     3061 tgcctggttt gcccactcat ttccgcatgg tcccggatca ttcatgtact taagtgaagc
     3121 aggtgtagcc cagcctgagc ccggtacagg aatccacgca ggctcccacc agaaaaatcc
     3181 ttttccgtgg tcatccacca catttgccac tctgtttaag aaatcctttg taaagtcagc
     3241 ctgtccctca atagtcatag gataatctat cttctcaaca agctccggtt ttgtcgcata
     3301 acccttgcgc tcagaatcag caagcttctc gtactcctgg tagctgtcca ttgtaaagcc
     3361 catggatacc tcagcgatga taaggtcctt attgaaacgc tttgcaatgt cattcatgtt
     3421 gaactcaagc tggtcaagtg agccgtgcca gaacggatag taagacaatc cgatatactc
     3481 aaactcctct cctctttcga tgaagtttgt aaaccatctg acataaagct cgttgtttcc
     3541 accattatcg aggtgaatca taatcggaat atcagcattt accttgcggc aggcacgtat
     3601 tccggcattc acaaattttg ctatattatc gtagttaggt acctttccct caggccacaa
     3661 aagtccgttt gagagctcat tacctacctg aatcattgtg acatttacac ccgcctcaat
     3721 aatctttgtc agattttcaa gtgtaaaatc atatacagcc tgctccagct cgtccgcgcc
     3781 gaaattcttc caagcctttg gcttaatctg ctttcccggg tctgcccaga aatcactgta
     3841 gtggaaattc aaaagcactc caaagcctgc atctgtaacc ttttttccga tagcgatagt
     3901 ctcagcaagg tcattgttac ccgcaccgta aggctcaccc tcctctgatt ttgggtcgtt
     3961 ccacagacga agacggattg tatcaacatc atgctccttc atgatgtcaa gaatatcctt
     4021 ctcctttcca tcctcgtagt attttgctcc gcagcgttcc agctcgagca aggttgataa
     4081 atccataccc ttaataaatt ttgacatttt aattctcctt aattcttata tcatttatcc
     4141 ttatatcatt tatttacacc atgagagcag tcccagtaat agtccctgct ctcaaaatgc
     4201 gtaataatat tccttacaac cttaaaatgc tagattccac ggaaacagaa tgtgaattcc
     4261 attttctctg aggcatcaag cagatactcc ggatgagtat gtgcgcccca gctgtcgtct
     4321 ccgcctacac ccatctgctt ctttgccaca cggacaacag tatagtgtac aggtggaagc
     4381 tcaaagtgat gtgcagcatt ttccagctca tgtggtgtat atggcagtgc cgagaacgaa
     4441 agctcatcac caaagaacag catgcctctg cccttcttat ccattacctt tgcatatctt
     4501 actctgcact tgttgccgct ctcctgcgga acgaggtact ctgcgacatt gtctgccact
     4561 ttattctcgt atacaccata ctttccgcca tgctgtctgt cctcgtatgt ctcctgtggt
     4621 ccgagtccgt accatttcac tgtatcataa tcggcatcca gcttgaacat catgccaaac
     4681 tccggcatat cgccaagttc ctttacaggg tcatatgaca gagttgtctc tattgttcca
     4741 tcaccaaaca ctctgtatgt cacctcgcat gaactctgcg gtgtggtagg catgaaataa
     4801 gtgtatgtga tggcgatatt gttatcattt ttttctaccc ttggtgatgt atccgcaaag
     4861 cgctgatcct gtctggtggt cacatacatg ctggcaatct tccactgtgc atagcgctgt
     4921 ggcatcatat ttccggaatc attgttggta ggtgttctcc agaaattcgg cagtggaata
     4981 gtatttggaa gcatctcgac gccaccatat acatatgaaa ccattcccag attcattctt
     5041 gagaaaagtg ccttgaagtt ctcacctctt acaaggatat ttccgtttcc aacaacaagc
     5101 tcaactgatt tgtcgcatga atactcactt acaggcttct ttatcatatg ctgtccgaat
     5161 gcaatctcat atcctgcctt ttcccagatg gtgttttcct tcaatacaaa tgatacgaca
     5221 atacagtact ctttttccac atctatcatg gaatttttaa gagtcaccgg cacctcatag
     5281 cttgccctat ccatcggtgg aacagatatc tgaagctttg tcctgtaaac ctcctcaccg
     5341 tctgcaagaa gaatggcaaa agcatcaaaa accgatgtat ttacaaagag atttttgttt
     5401 gtgactgtaa agatgtagtc gctgtcaaaa tcaaccgata tattctgata attaaacttc
     5461 acctcctgca tctttggaga agcctctctc tctccgccat aacagatgcc gtttccgctg
     5521 aaatctccat ctgtaggcct gtcgccgaaa tctccgccat atgtgaggaa ccacttgccg
     5581 tatctgtcct ttttgtaaat tgactggtca atataatccc agataaatcc accctgatat
     5641 ctaggctctc ggtcagaaag ctctgtgtat ttatgcatag caccgcagga atttcccatt
     5701 gcatgtgtat attcgcagca gataaacggt ttctcaggat gctctgccag gaatttttca
     5761 atgcctgcag ccggagtata catctggctt tccatgtcac ttgtgtcatt gtagcttcta
     5821 tcgttaaaca ctccctcata atgcaccaga cgatgcttat caagctgtct gaaaagctgt
     5881 gacatctcat atatggtctt gccgccaaac gactcattgc cgcatgacca gataaggatt
     5941 gccggatgat ttttatccct ctgatagcat gagttggcac ggtccagcat catttctctc
     6001 cactgtggct tgtcctttgg cacaacaaag tcaaggtcaa catagccctt attatatgcc
     6061 tcccatgtgc catgactctc aagattgttc tcagctatca tatagattcc ataaatatcg
     6121 caaagcttat agagcatgga atcatcagga taatggcttg ttcttatcgc attgatattg
     6181 ttctttttca ttgtgacaat atcctttatc acctcatcac ggtttggcac acggcctgtt
     6241 attgagctga actcatggcg gtttacaccc ttaaatacaa tacgcttacc gtttagcttc
     6301 atgagtccgt ctaccatctc aaatcttctg aagcccgcca gctggctgat aaactcggtc
     6361 tcatttccat gcgtatcttt aactataatt ttaacctcat acagattcgg cttttcagcg
     6421 ctccataaca gaggattttt cacattacat gagccctcgc aaatttcctt attcctaagc
     6481 tctatatcaa actcttccaa taaaagtttt gccctgtcat atttttccac agacaaatcg
     6541 cccagctcat aaagctttac acttgcaata ccatcaccat ctgcctttat tgacacacta
     6601 agtgtgccct cgtcaaaggt atcattaagt gtaggaacaa ctgacagatc ctccacatgt
     6661 gcacaaggaa cagcatacaa aaatacatct ctgaaaatgc ctgaaaatct gtaaaaatcc
     6721 tgatcctcac accagctgct tgatgtccat ttccataccc tgaccgcaag cttgttctca
     6781 ccctcgacaa tataatcagt cagctcgaaa tcagacggat caaatgtgtc ctcactgtag
     6841 ccgacatagt gtccattcag ccagagtgca aaaccgctct ccactccctg gaacgaaata
     6901 cacaccctct tgttcttcat attctcagga attgtgaaat acttcacata gcttgcaacc
     6961 ggattaaaaa tctcaggtac ctcgcccggc tttatagact catcagcctc ccacggatat
     7021 gtggtattcg tatagattgg cacgtcatag ccttccatct ggatatgtgc cggaaccctg
     7081 atttcatccc agcctttgca atcatagtca gctgcctcaa agccacaagg agccagggac
     7141 tgattctttg catatgcaaa cttccataag ccgttcatat catatctcag gcttgatttc
     7201 cctttaattt tctccagttc atttttatag cacacatgat ccgaatgagc cgcaagtctg
     7261 ttctgctcaa atatttcagg atttttaatg atatttgaat caaaattact catattttta
     7321 cactcctgtc tactgtattt attatttgat acatgtctgt tttaaacaca aaaccctctt
     7381 ataagacgaa agcatgtatt ttctaccatc ccttt
//
LOCUS       DPUF01000110            5683 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7435, whole
            genome shotgun sequence.
ACCESSION   DPUF01000110 DPUF01000000
VERSION     DPUF01000110.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7435"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="DHV81_04075"
     CDS             complement(<1..122)
                     /locus_tag="DHV81_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HCI93675.1"
                     /translation="MKNIVNNYSSIEQLTNQYLNKQKSTDKKINGISFEEILKQ"
     gene            complement(131..844)
                     /locus_tag="DHV81_04080"
     CDS             complement(131..844)
                     /locus_tag="DHV81_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HCI93676.1"
                     /translation="MAITANVTNGKLDKTNTTPTQNRKTSSAKASGSNLGYDDFLKLL
                     SAEMQYQDPLEPTSNTDYVAQMATFSQLEATLSMKESMASSNDQTTKSAALSLVGKEV
                     IVTDKDSASGYYSGKVDYVTYKDGKIQLSINEKMYDYSSLYSVSTDEYYDAIVNSSTF
                     SSLIAKLPKIEDLTIDSKGSIEEARKLYDGLSDYGKQFINASDYSKLQAYEDKLKELI
                     AADKNNQADSKENDTNQTA"
     gene            complement(868..2309)
                     /locus_tag="DHV81_04085"
                     /pseudo
     CDS             complement(868..2309)
                     /locus_tag="DHV81_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742644.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
     assembly_gap    1686..1726
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2350..3219)
                     /locus_tag="DHV81_04090"
     CDS             complement(2350..3219)
                     /locus_tag="DHV81_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93677.1"
                     /translation="MAELNESESTKDTKKTKKEKKMEEKAAKKAAKKAKKNHEADKPD
                     AENDDSDSVSSKIVMVLVTILIIAIWLGIFAVFVKADVGGFGSTVLAPALKDVPVLSK
                     ILPDSGNITASESTETAGYTNLDDAVSRIKELEKQLDDANSKNQADAQTINDLNAQIQ
                     DLSTYKEEQAAFEEEKEKYYEEVVFSDKAPDIKEYKKFYEQVDPANAEILYKQVCEQL
                     EQDDEIKDYAQTYSKMKPKEAAAIFDSMTDNLSLVAKILNAMDAQSRGNILGKMDSAT
                     AAKVTKIMNPSQN"
     gene            complement(3232..3669)
                     /gene="fliJ"
                     /locus_tag="DHV81_04095"
     CDS             complement(3232..3669)
                     /gene="fliJ"
                     /locus_tag="DHV81_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HCI93678.1"
                     /translation="MGKFKYSMQNILDIKIKLETQAKNEFSEANRAYLIEKEKLEELF
                     KKRAVYEAALKEQLSGSLNISEINICKKNINSLKSAIRTQLMNVKKAEDMLEVKRVAL
                     NELMKERKTHEKLRDKKFEDFQKEEKDNESKEIDQLVSFTYNS"
     gene            complement(3671..4984)
                     /gene="fliI"
                     /locus_tag="DHV81_04100"
     CDS             complement(3671..4984)
                     /gene="fliI"
                     /locus_tag="DHV81_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HCI93679.1"
                     /translation="MLEVAQKYLQLKSGEYYKCFGKVDKVVGLTIESIGPKAKLNDLC
                     MIFLDKERRDYVMSEVVGFKDSHIILMPFDNVEGVGVGCIVENTGHPLTVAVGDELLG
                     HTVDGIGRVTDCDEKDLKLDSEYPIEAPPPDPMSREIISDVLPLGVKAVDGLITVGKG
                     QRIGIFAGSGVGKSTLLGMFARNTKADINVIALIGERGREVREFIERDLGPEGMARSV
                     VVVATSDKPALIRNKAAKTATSIAEYFRDKGEDVLLMMDSLTRFSMAQREIGLASGEP
                     PVTRGYPPSVYSEMPKLLERAGRADKGSITGLYTVLVDGDDFNEPITDTARSILDGHI
                     MLDRKLGHKNHYPAIDVLQSISRVMSAIATKEHKNLAGRLKNVLATYTEAEDLINIGA
                     YKNGSNPDIDYAIQKIGAVNSFLCQGTDEKFSFEEELELLEKVFE"
     gene            complement(5021..5443)
                     /locus_tag="DHV81_04105"
     CDS             complement(5021..5443)
                     /locus_tag="DHV81_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis/type III secretory
                     pathway protein"
                     /protein_id="HCI93680.1"
                     /translation="MSLRQELSDKESSLKSKYDAYSKELESDLIDAIIQVFNKVFKIQ
                     FDDKKDILFHLVENTMSNIEVGKEFRIHVAYSNYKFMMSHLDEIQERIGNDIDIEVVN
                     DANLDTSGCFIETSFGVFDCGIDMELNNLVKDIRSLCS"
BASE COUNT         1392 a         1323 c          993 g         1934 t
ORIGIN      
        1 tgctgcttta atatttcctc aaaggatata ccatttatct ttttgtctgt agatttttgc
       61 ttatttagat attgatttgt cagctgctct atggatgagt aattattaac gatattcttc
      121 ataatccacc tcacgcagtc tgattcgtat cgttttcttt actgtcagcc tgattatttt
      181 tatctgccgc aataagctcc ttaagcttat cctcataagc ctgaagcttg ctgtaatcac
      241 tggcatttat aaactgcttg ccataatccg aaagtccgtc gtacagcttt cttgcttcct
      301 ctatgctgcc ctttgaatct atcgtcagat cctctatctt tggaagcttt gctataagac
      361 ttgaaaatgt acttgagttt acaattgcat cgtagtactc atccgttgat acagaataga
      421 gactcgagta gtcgtacatt ttttcattga tagacagctg aatcttgcca tccttatatg
      481 tgacatagtc taccttgcca ctgtagtatc cggaagcact atccttatca gtaacaataa
      541 cctccttgcc tacaagtgaa agtgctgcac tctttgtagt ctggtcattt gatgatgcca
      601 tggattcttt catagaaaga gttgcctcca gctgtgagaa tgtagccatc tgtgccacat
      661 agtcggtgtt gcttgttggt tcaagcggat cctggtactg catctctgca cttaaaagct
      721 ttaagaaatc atcatagcca agatttgagc cacttgcttt tgcactgctt gtttttctgt
      781 tttgtgtcgg agtggtattt gtcttgtcta gctttccgtt tgtaacattt gctgtaattg
      841 ccatttgtcc tcctttttca tgacacctta tgctgtgaaa tctactgaat ttccgttatc
      901 tctcataatc tgtgtcacaa gtctttcttc agcgcgatcc tgcatcatat cgctgtctat
      961 gccgctgtca gagctgtttg aaccattata ggaatttgtg ctctcctgct gtctgctatc
     1021 gtttgcagca ccctgctcga gattcctctc aaactcatgt gatgcaacgg tgatttccac
     1081 ctcatttacc ctgatgcctg aattgtttaa ggcttccttg agattgacca tctgtgtctg
     1141 taaggcttct tttacagcct cattggaaac tgcaattctt gcagatatct cgctgttctt
     1201 ctgagatacg ttgatataca ttcttccaag gctttcaggt gtaagctgaa ggttaattgc
     1261 cgattcttca gtggtgtttg atatgctcat actgtcgaca atctgtctga tgatatcggt
     1321 cgtatcaaca cgtaatcggc tttgtgactg tgacatttca aatgaagtat cagtattcag
     1381 ctgttcgctg acagatgcat gaagtactga attgtctgag tgtgcgtcat catcatgatg
     1441 caacacgcgc tcacctgaat catcaaagct actgtcaacc tgagactttg actgagtctt
     1501 ttctgtattt tcatctgatg taataccatt gcctgtttcg cctgaagaag tgccttcgtc
     1561 cgagttttgg taagaactgt cctgtgtacc gccatcatat accttaacag cttcatcagc
     1621 agtgtcgttt atattctgac ccgaagctgc tgtatcgtca tagctgctgt caaccgtttt
     1681 ttccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaga aactatatct
     1741 gcatctagta tttcaccatc atctgaagca gtaagctgtg ataataaatc aagctgtgca
     1801 tcattttcag atgcaacctg agacacatca ttaaacagtg cagtaaagtc ggcatttaaa
     1861 acaagtgcct gtggatcaga tgcatttccg gtgacctcac aatataatgc cgacagattt
     1921 gcagggttga gtaaatccat agctgtcagt ccaagaagct gcattgcatt attaagttca
     1981 tcctcagata tatcgaggtc atcagttact ttttcaacta tctgcgaaga tatatcatcg
     2041 gtgatttttt taacttcgct ttctgtaggg ctgttttttg atgcagtttt gatgcttatt
     2101 tccttagttt tgtttttatt taaagtataa gagtttcttc gctttaaaaa tgtagtgctg
     2161 ttatcctgca cagctgtccg gccgccaaga tagcttttaa agtcaatacc ggattgtgca
     2221 gatgtttgcg ttacagcctt ctgccctgtc gaaacagtcc gtccacctga tacatttggt
     2281 acaacaggtg atattgtatt gtctgccata ttctcctcct ttcttttatt attgttacca
     2341 gatatgctat cagttctgtg aaggattcat aatcttagta acctttgccg ccgtagccga
     2401 atccatttta ccaaggatat tacctctcga ctgggcatcc atggcattta atatcttggc
     2461 aacaagtgac agattgtcgg tcatgctgtc aaaaatagct gcggcctcct taggcttcat
     2521 tttggaatat gtctgggcat aatccttaat ttcatcatcc tgttcaagct gttcacacac
     2581 ctgtttatat aagatttctg cattggcagg atctacctgc tcgtagaatt tcttatattc
     2641 ttttatatcg ggtgctttat ctgaaaaaac aacctcctca tagtattttt ctttttcctc
     2701 ctcaaaggct gcctgctcct ccttataggt gctcaaatcc tgtatctggg catttaaatc
     2761 attgattgtc tgggcatctg cctgattctt gctattggca tcatccaatt gcttttcgag
     2821 ctccttaatt ctcgaaaccg catcgtccaa atttgtgtag cctgcggtct ctgtagactc
     2881 agatgcagtg atattgccgg aatcaggaag tattttactt agtacaggca catcctttag
     2941 tgccggcgca agcacagtcg agccaaagcc acccacatct gcttttacaa aaactgcaaa
     3001 tattccaagc catattgcaa taataagtat agtaacaagc accatgacta tcttgcttga
     3061 gacagaatca ctgtcatcat tttctgcatc gggcttatcg gcttcatgat tcttctttgc
     3121 tttcttagcc gcctttttag cggctttttc ttccattttc ttttcttttt ttgttttctt
     3181 agtatccttg gttgattctg attcgtttag ttctgccata tttcctccgg actacgaatt
     3241 ataggtaaag ctcacaagct ggtctatctc tttggattcg ttgtcttttt cctctttttg
     3301 aaaatcctca aatttcttat cgcgcagctt ctcatgcgtc ttacgctctt tcataagctc
     3361 attcaatgcc actcttttaa cctccagcat atcctccgct ttctttacat tcataagctg
     3421 tgtgcggata gccgatttga gtgaatttat gttttttttg caaatattaa tttcagatat
     3481 atttaagctg cctgaaagct gttccttaag agcagcttca tagactgcac gctttttaaa
     3541 caactcttcg agcttttctt tttcaatcag ataagcacgg tttgcttccg aaaactcgtt
     3601 ctttgcctgt gtttcaagct ttatttttat atcaagtata ttttgcatgc tgtatttgaa
     3661 ttttcccata ctactcgaac accttctcta aaagctcaag ttcctcttca aagctgaatt
     3721 tctcatctgt tccctgacaa aggaatgagt taacagcacc aattttttga atggcatagt
     3781 cgatatccgg gtttgagccg tttttgtatg caccgatatt gataagatcc tcagcctccg
     3841 tgtatgtggc tagcacgttt tttaatctgc cggcgagatt tttgtgctcc ttagttgcta
     3901 tagcactcat aactctcgaa atactctgca gtacatcaat agccggataa tgatttttat
     3961 gtcccagctt tctatcgagc atgatgtggc catccaaaat agaacgcgcc gtatctgtaa
     4021 taggctcatt aaaatcatct ccatcgacaa gcacagtgta cagccctgtg atagagcctt
     4081 tatcagctct tcctgctctc tctaaaagct ttggcatctc agaatacaca cttggtggat
     4141 atcctctcgt cacagggggc tctccggatg ccagtcctat ctctctttga gccatggaaa
     4201 aacgtgtcag tgaatccatc atcaaaagca cgtcttcacc cttgtcacga aaatattcag
     4261 cgatgcttgt agctgtcttt gcagccttgt ttcttataag agccggcttg tcagaggttg
     4321 caactactac aacagagcgt gccataccct cagggccaag atccctttct atgaactctc
     4381 taacctccct gcctcgctct ccaatcaggg caattacatt gatatctgcc tttgtgttgc
     4441 gggcaaacat accaagaaga gtacttttac cgactcctga gcctgcaaaa ataccgatac
     4501 gctgtccttt gcctactgtg atgaggccat ctactgcctt cactcccaga ggaagcacat
     4561 cactgataat ttctctggac atcggatcag gtggcggtgc ttctatcgga tattcactgt
     4621 caagcttaag gtctttttca tcacagtcag ttacacggcc tattccgtca actgtatggc
     4681 ctaaaagctc atcaccgaca gctaccgtga gaggatgtcc tgtattttca actatacagc
     4741 cgacaccgac tccctccaca ttgtcaaacg gcatcagaat aatatgtgaa tctttaaatc
     4801 ctaccacctc agacatgaca taatccctgc gttccttgtc aagaaaaatc atgcaaagat
     4861 cattgagctt agccttggga ccaattgatt ctatcgtaag accgacaacc ttgtccacct
     4921 tgccaaagca tttatagtac tcaccgcttt ttaattgtaa atacttttgt gctacctcta
     4981 acaattaaaa tccctttctt ccaacactca tacagctata tcagctgcaa agtgagcgta
     5041 tatccttcac cagattgttg agctccatat caattccaca gtcaaacact ccaaagcttg
     5101 tctcaatgaa gcagccgctc gtgtcaagat ttgcgtcatt tacaacctcg atatcaatat
     5161 cattgcctat gcgttcctga atctcatcca aatgcgacat cataaactta tagtttgaat
     5221 aagctacatg tatacgaaat tctttgccca cctctatatt tgacatggta ttttcaacca
     5281 gatgaaacaa gatatccttt ttatcatcga actgtatttt aaagaccttg ttaaaaacct
     5341 gaattattgc atctatcaga tctgattcaa gctcctttga ataagcatca tattttgatt
     5401 tcaaagagct ttctttatct gaaagctcct gtcttaaaga tatttctttt tcgtcaaact
     5461 ctctctgaca ctcagccaga ccatcctcat agccctttga cttgttatca gcatatagaa
     5521 tctgtgcacg ttgtttagcc tcatctaaaa gtcttgaggc ctctgccttt gctttgtcca
     5581 atatgctatc agcctcaagc tttgcttcct ctaagagcct ttccttctca gctgaaacat
     5641 ctatttctgt gactacagca gcatttatgc catcattgaa gcc
//
LOCUS       DPUF01000111           12528 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3202, whole
            genome shotgun sequence.
ACCESSION   DPUF01000111 DPUF01000000
VERSION     DPUF01000111.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12528)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12528
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3202"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>413)
                     /locus_tag="DHV81_04110"
     CDS             complement(<1..>413)
                     /locus_tag="DHV81_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCI93681.1"
                     /translation="TIAMAFDECPSSVADRQYVTNSVNRTARWLERCKKEMARLNSLP
                     DTINKEQLLFGINQGAIYDDIRIDHAKRISELELDGYAVGGLAVGESHEEMYHILDET
                     VPHLPVNKPTYLMGVGTPANILEGVERGIDFFDCV"
     assembly_gap    414..560
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<561..850)
                     /locus_tag="DHV81_04115"
     CDS             complement(<561..850)
                     /locus_tag="DHV81_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCI93682.1"
                     /translation="MAKYELLKQEGRAKRGTFHTVHGDIQTPVFMNVGTVGAIKGAVS
                     TDDLKEIKCQVELSNTYHLHVRTGDKIIKELGGLHKFMVWDRPILTDSGGFQV"
     gene            complement(877..2652)
                     /locus_tag="DHV81_04120"
     CDS             complement(877..2652)
                     /locus_tag="DHV81_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HCI93683.1"
                     /translation="MEFNGVFHQAYDNYCYPLNEDELIINIKTGYDVKEVNIILGDPF
                     AAGILGGGETWNGDKQPIIFKKKLKHQIWWTTTVKPPFKRLKYYFELITEDERWFYFE
                     DGFVSAEQMALEGRSRQCFVFPWMNPCDIPVTPKWVNDTIWYQIFPDRFCNGDHSIDP
                     DYVVPWRNRGKVKNEECFGGDLAGIIQKLDYLKELGINGIYLTPINESPSNHKYDTTD
                     YAKIDPRFGDEDTFKRLVLEAHKRDMRIMLDGVFNHCGYYFAPWQDVLAKGADSEYYD
                     WFMINEWPLDFKHGAAKKGQFYTFGFFDNMPKLNTNNPAVRKYFIDICANWVENYHID
                     GLRLDVANEVSHRFCKELRARLKEINPDIYILGEIWHNALPWLRGDEFDAVMNYPLGE
                     SIKDFWIDKSQTNQDFEYTINRCYTNYMQQTNDVLFNLLDSHDTKRLRSDTKNLDQYF
                     AQLAVLFAMPGSPCIYYGTEIAMPGSYDPDCRRCMPWDDIEAGRFKERIEMVSKLIHL
                     RASEPLLKGRNFHFPDDFADNPRVIQFRKMGWVDTYVEVIVNCSDEDIEVPKDGEVLF
                     ERHCIDTTLLTNGILIRKIVGNGDK"
     assembly_gap    2922..2952
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3209..4312
                     /locus_tag="DHV81_04125"
     CDS             3209..4312
                     /locus_tag="DHV81_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCI93684.1"
                     /translation="MAGSSYGNIFKVTTWGESHGKGLGVVVDGVPAGLELCEEDIQIF
                     LNRRKPGQSKFTTPRKEDDAVEILSGVFEGKTTGTPVSMVVWNKNQKSKDYSEIASYY
                     RPGHADFCFDEKYGFRDYRGGGRSSGRETIGRVAAGAIAVKMLSGLGIKFLTYTRSIG
                     PVTINEVNFDAHEIYNNSLYMPDADAALKASEYLEECLKSLNSSGGVCECVITGLPVG
                     LGDPVFEKIDANLAKAIMSIGAVKGFEIGDGFDAAKATGLTNNDAFIMRDGAVSKETN
                     HSGGTLGGMTDGSPLIFRAAIKPTPSIAATQHTVNKSGEDIEISIKGRHDPIIVPRAV
                     VVVEAMAAITIADALLMNMGARMDRITDFYKKL"
     gene            complement(4422..6527)
                     /locus_tag="DHV81_04130"
     CDS             complement(4422..6527)
                     /locus_tag="DHV81_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI93685.1"
                     /translation="MDIVKNEICKLLTKRTVLILLFLLVLNPVLGLYTMNTVNDDGYT
                     DKDYSALYGEISNYSREEVLPEIEQRQMTAETYGRISLCSRVYKEVGACLSYDEYLDS
                     VNEKADEISIMNKFSGNGGFAEKNAAKTSRVYSKLKGTVPEVIDASGLLNITDNELTD
                     YVAVIMLFIIALNLVFYEKSENQLALLRTTARGRRQLMASKSFVMIMAVILITLLLYG
                     INAVISMCFYNPINLKSPLQSVYLYYGSPFKLSIGQFLACYFPVKIISFILLGLFFML
                     ICAALDNIIFVFVASAVTVVIEAICYTTISGTSFLAFLKYINIMYGVRTGRLFSDYVN
                     INLFGYPLNTGVLYGLFWLVCIAVCIFAVTNYLNSVHEKKLLLLPGFACGKNTGCHTS
                     LFLHECYKALVPGKVLLILIVAAIFVVWWNPAEKLSYDSVDEVYYKEYMDKYYGPLTA
                     KTNELLDEERAIYDRLSDNIAYDMAQGKSESYINIKYKDELSRQEAFNKLTAHVDYLK
                     TLNEGWLFFEKGYDILTDRTDFKNRDTAQAFVYVILLIAIACGICGADYANHEIRLLR
                     TTRRGRKQHMGIKCILGFLGTVTAFALVYIVRLCNVLSAYGTQGLNAPAASMEHLWRV
                     SQNISVGQYILIIMLMRFIGGLLVSLFVFAMFKYLRSGMSVIISCVLFIVLPLALVAF
                     GVTNAQYMLLNPLLIGNIF"
     gene            complement(6530..7393)
                     /locus_tag="DHV81_04135"
     CDS             complement(6530..7393)
                     /locus_tag="DHV81_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93686.1"
                     /translation="MTLELEGLTKVYKDKTALDDVSFSFTPGIYGLLGPNGAGKSTMM
                     NLISDNLTPSKGRVLLDGRGIDELGSEYRKLLGYMPQQQNIYPELSLRRFLYFMASLK
                     GLKKSEAWEDIDHYVRMVKLDDVLGKKLGAFSGGMKQRALIAQALIGNPGILILDEPT
                     AGLDPKERIRIRNLISEVAKDKIVIIATHVVTDVEFIAKEIVMLSNGKIIRSGSPSKL
                     LQELDGKVWNIFLSDNEIDEVNTGYKVSNISRDADGVIARIIADSYDGRWQKEAVRPN
                     LEDLYLYLNGD"
     gene            complement(7390..8889)
                     /locus_tag="DHV81_04140"
     CDS             complement(7390..8889)
                     /locus_tag="DHV81_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCI93687.1"
                     /translation="MMKNKKKGVLIAVVVIALIVAAVAAVCINKKAGDSGKLKEGQTE
                     TTVKKSDTKVITFAVPDICRIDENNLKQFNAALMSDGYNYELNIKYLEYDEYSQKLES
                     AFAAGETDVAFLGLGDENGNNPVYDLISSDLVLNLDDVLSKDQGKTLYDAFPKNLWEM
                     AKCDGHIYSIPSALADDNGVYAAFNRDYISDDVINSWDGSIDGIYQILKASEWDNSKA
                     PGFQYLINEYVFGDMIGCEIRNGLCFDYDTMSVENPLESQKFTEYLKGLDKMKKDGYL
                     KDDETGEITYLNNIGLNNEEILQNVKSGNFVVALCSGEIDENFQKDNIAVKTVEHYLT
                     SRVNASIGIAKNTKDVDAVVEFLGLLYGNGKYADILLYGQEGTDYKVVDGVACNTDGT
                     DLEDDYLSKLCLDLFINVYPVTGERYMENRKDEFFALYDNAGVSPFIGFEPDTENLNN
                     ISNDLSDFMNGLNGKSVEESIEGAKEKLTADGMDSYLDSVRNQWEEYKQ"
     gene            complement(8876..10105)
                     /locus_tag="DHV81_04145"
     CDS             complement(8876..10105)
                     /locus_tag="DHV81_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93688.1"
                     /translation="MKKKRILIASIIFILIICIVAAVFIIKNRNRSTSNTMYTYSNVD
                     PEYNYSEDNVFYFDDSGILHLIDTASGKDIVYCDKPNCTHEGYSRTNQNPSCPAAFYG
                     LSKAGAVIYNDHLYFIGNMSDEDMTIQYLYVMDSNGENRKKTAKLENVQNVRAVLYRD
                     NYVIGAYSNRVELNDEGQIINDDKPEAGIFVIDLDNYKVYMSDKITSEQANIMGIYYE
                     DGAVYYSTVRFGDDVTELMIEEGATADAESFAYDNMLNGIYQYDIADEKTTCLTEIDH
                     INNLRLLDGDGYYSSKDGYFVFDTESRQTRQLPIDADGKTQYGPLKKSGDFLYYALSE
                     ENSDEVTYYRLKDDKSEELMKLSTEKAFGIENICGQSVYVNYTDDEGEFSLGVISLDN
                     LNKGNFNPRKLRCYNEE"
     gene            complement(10147..10539)
                     /locus_tag="DHV81_04150"
     CDS             complement(10147..10539)
                     /locus_tag="DHV81_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93689.1"
                     /translation="MNKITKANFKKLVLALALTLVMTLGMSISVFAATGAINGYTTRA
                     SSTIRQQKASASTSYDYNGSVSVSSTYSYVDVNTLATGTYTKNNAHYSHCSVEFSAPS
                     NCHSVKIVSSHKVSAFGQIWSTKTSATC"
     gene            complement(10593..10988)
                     /locus_tag="DHV81_04155"
     CDS             complement(10593..10988)
                     /locus_tag="DHV81_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93690.1"
                     /translation="MNKITKANFKKLVLVLTLTLVMTLGMSISVFAATGAINGYAITG
                     SSHITRTTASASTTYEKRTGSISVDSTYSYVNTYTLATGSSTKSKGYYTSVEINFSAP
                     YNCHSVRIRSSHKVSAYGQTWSSNSTAVY"
     gene            complement(11020..11181)
                     /locus_tag="DHV81_04160"
     CDS             complement(11020..11181)
                     /locus_tag="DHV81_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCI93691.1"
                     /translation="MNSLKRLTLELVKKVVIFEIKRDNETHSFCACILYQPKRPEKGR
                     FQVTNNRNI"
     gene            complement(11174..11764)
                     /locus_tag="DHV81_04165"
     CDS             complement(11174..11764)
                     /locus_tag="DHV81_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HCI93692.1"
                     /translation="MVKNIVNAIVEQMEKENLLNIEMKEYFTYSIAMIIEKWITIISV
                     LFVGGIFKQTVQMMLFLFFFLLLRKRTGGYHASSFLHCYIETIIISIVVIHMCLISAR
                     YMMVIYLLVICSILVISFIGVVNHPNLALDYVEVNESKKAARCILAIESIIIVLLIVL
                     DVCRLCVCYMSASIILCALLLGLAKIIKQEVKFENE"
     gene            complement(11876..>12528)
                     /locus_tag="DHV81_04170"
     CDS             complement(11876..>12528)
                     /locus_tag="DHV81_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCI93693.1"
                     /translation="AKLHEKEVLGLQFKNQIDMYRSISENYEIQKKKSHEFKNHILCI
                     ESLLKAHEYDEASKYVRNISNTFIGEKNAINTNHVIINAILNTKYQEAISKNIVFVFK
                     VNDLSKIVIKDEDLVVILANLLNNAIEACEKCEEKKTIKLKFVIEENLIVLSVKNTCS
                     NLIIYSGNEIKTSKKDEPEMHGIGIKNIIQIVEKYNGEYVIKNENNEFSFSIIIPL"
BASE COUNT         3558 a         2815 c         2012 g         3965 t
ORIGIN      
        1 tatacacagt caaagaagtc gattcctctc tctacaccct caagtatatt ggcaggagtt
       61 cctactccca tcaggtatgt aggtttatta acaggcagat gaggcactgt ttcatcaaga
      121 atatggtaca tctcctcatg gctctctcct accgccagtc cgccgacagc atagccgtcc
      181 agctcaagct cagaaatgcg ctttgcatgg tcaattctta tatcatcata aatagctccc
      241 tgattgatgc cgaataaaag ctgctccttg ttgatagtat ccggtaagct gtttaagcgt
      301 gccatctcct ttttgcacct ctcaagccag cgcgctgtcc tgtttacaga gtttgtcaca
      361 tactgtctgt ctgcaacact agatgggcac tcgtcaaatg ccatagctat cgtnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn acctggaatc cgccggaatc tgtaaggatt ggtctgtccc
      601 acaccatgaa tttgtgcagt ccgccaagct ctttaatgat tttgtctccg gttcttacat
      661 gcaggtgata tgtatttgac agctcaacct gacactttat ctcctttaaa tcgtccgtag
      721 agacagcgcc ctttatcgca cctactgttc ctacattcat aaatacaggt gtctgtatat
      781 ctccatgcac agtatggaat gtacctctct tggcacgtcc ctcttgtttt agtaactcat
      841 atttagccat attttcgttt aattctcctg tttcttttac ttatcgccat tgcctacgat
      901 ttttctgatt aatattccgt tagtcagcag tgtggtatct atgcaatgcc tctcaaaaag
      961 tacctcgccg tcctttggca cctcaatatc ctcatcagag cagttgacta taacctccac
     1021 atatgtatcc acccagccca tctttctgaa ctggattacc ctcggattat cagcaaaatc
     1081 atcagggaaa tggaagtttc tgccctttaa aagcggctca ctcgccctta aatgaatgag
     1141 ctttgatacc atctcaatgc gctccttaaa cctgcctgcc tcaatatcat cccacggcat
     1201 acatcgtctg cagtcagggt catagctgcc cggcatggca atctcagtgc cgtaatatat
     1261 acatgggctg cccggcatgg caaaaagcac tgcaagctgt gcaaagtact ggtcgagatt
     1321 ctttgtatca gagcgaaggc gctttgtatc atgtgaatca agcagattga aaagcacatc
     1381 attggtctgc tgcatgtagt tggtataaca tctgtttata gtatactcaa aatcctgatt
     1441 cgtctggctc ttatctatcc agaaatcctt gatgctctct ccaagcggat agttcataac
     1501 cgcatcgaac tcatctccac gcagccacgg aagagcatta tgccatattt ctcccaaaat
     1561 gtaaatatcc ggattgattt ccttaagcct tgccctaagc tccttgcaga atcggtgtga
     1621 cacctcattt gccacatcaa ggcgcagtcc gtctatatga taattttcta cccagttggc
     1681 gcagatatcg ataaagtatt ttcgcaccgc cggattattt gtattgagct ttggcatatt
     1741 gtcaaaaaat ccaaacgtat aaaactgacc ctttttagcc gctccatgct taaagtcaag
     1801 aggccattca ttgatcataa accagtcata atattctgaa tccgctccct ttgctaaaac
     1861 gtcctgccac ggtgcaaaat aatagccaca gtggttgaac actccgtcaa gcattattct
     1921 catatcacgc ttatgggctt ctaacacaag cctcttaaag gtatcttcat ctccaaagcg
     1981 cgggtcaatc tttgcataat ccgttgtatc atacttatga ttgctcggtg actcgtttat
     2041 cggtgtcagg tagattccgt ttattccaag ctcctttaag taatcaagct tttgaatgat
     2101 tcctgcgagg tctccgccga agcactcctc atttttgacc tttcccctgt ttctccacgg
     2161 aacaacataa tcaggatcta tcgagtggtc tccattgcag aatctatccg gaaatatctg
     2221 ataccatata gtgtcattta cccacttcgg tgtgaccgga atatcacacg gattcatcca
     2281 cgggaataca aagcactggc ggcttctgcc ctcaagtgcc atctgctctg cactcacgaa
     2341 gccatcctca aagtaaaacc atctctcatc ctctgtgatg agctcaaaat aatatttaag
     2401 tctcttgaat ggtggcttta ccgtggtggt ccaccatatc tggtgcttta atttcttttt
     2461 aaatattatc ggctgcttat cgccattcca ggtctcacca ccgcctaaga taccggctgc
     2521 gaatgggtca ccgagtatga tatttacttc cttcacatca taaccggtct ttatatttat
     2581 gattaattca tcctcattta aaggatagca gtagttgtcg tatgcctggt gaaatacccc
     2641 gttaaattcc atgttttctc cctcttttgc tgtcttattg tagttgttta tcattgtggc
     2701 gctaaggacg gcacgtaagt cgcggtcacc gtgagcgtcc acgcgctgcc ctgttcgcta
     2761 cgctgcgctg ttaagattct gtacgaatta tgcttataga ttttctccgg ctgacgaaac
     2821 cgtcgctact cgccctccat ggctcgatag ctccttgccc cggcgtccct gccggtgcat
     2881 ttctgtgtat cgaagcataa ttctacagac tctaaacagc tnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nngcaagcat gcatcgatga taccttttga ccctttaatc acataattat
     3001 atacaaacat agttcgaaag ttatctaaat aaaataatat catagttttc ttgactattc
     3061 aacttgctat taattataat gtatttatta tcgtaactat tcgagtcaca ttgaaaacca
     3121 aaaatgctgt ttacatgcaa gcatgatgta tcattttttg ttctcagagg tttaaatagt
     3181 tacccattat tataataagg agaccatcat ggcaggttca tcatacggaa atatttttaa
     3241 ggtaacaaca tggggagaat cacacggaaa gggcttaggg gtcgttgtgg acggcgttcc
     3301 tgcggggctt gagctgtgtg aggaggatat tcagatattc ttaaacagaa gaaagccggg
     3361 acagtctaag ttcactactc cacgcaagga ggatgatgcg gttgagattc tctccggtgt
     3421 gtttgaggga aagactacag gtacacctgt ttctatggtt gtgtggaata aaaaccagaa
     3481 atcaaaggat tacagtgaga ttgcttcata ctatcgtccg gggcatgctg acttttgctt
     3541 tgatgagaaa tacggcttca gagactacag aggcggaggt cgttcttcgg gacgcgagac
     3601 aataggccgt gttgcagcgg gtgctatcgc tgtaaagatg ctctcgggcc ttggcataaa
     3661 atttcttaca tacaccagat caataggtcc tgttacaatt aatgaggtta attttgatgc
     3721 ccatgaaatt tacaacaatt cactttatat gcctgatgcc gatgcagcgc taaaagcttc
     3781 tgaatatctg gaggaatgcc ttaaatcact caattcctca ggcggtgtgt gtgaatgtgt
     3841 catcacaggt cttcctgtcg ggctcggtga tcctgtattc gagaaaattg atgccaatct
     3901 ggcaaaagct attatgtcta tcggagctgt aaagggcttc gaaatcggcg atggctttga
     3961 tgcagcaaag gcaaccggac ttacaaacaa tgatgccttt attatgaggg atggcgctgt
     4021 ttcaaaagag acaaatcact ccggtggtac tctcggcggc atgacagacg gctcaccgct
     4081 tattttcaga gctgccataa agcctactcc ttcaatcgca gctacacagc acactgtaaa
     4141 taaatccggt gaagatatag agatttccat caagggacgt catgacccga ttatcgtgcc
     4201 acgtgctgtt gttgttgttg aagccatggc tgcaattact attgcggatg cactgcttat
     4261 gaatatgggg gcacgcatgg accgcatcac agatttttat aaaaaattat aactttgtcc
     4321 tatagtaaaa agcatctatc cggttttttc cggacagatg ctttttatct atttcattgg
     4381 aacaatctgt aatgtttttc ctagtgaatt caaaatatat tttagaatat atttccaata
     4441 agcagcggat ttaacaacat atactgcgca ttcgtcaccc caaaagccac aagcgccagt
     4501 ggcagcacaa taaaaaggac gcatgatata atcacagaca tgccgcttct taagtattta
     4561 aacattgcaa aaacaaacag gctgaccagt aatcccccta taaagcgcat cagcattatg
     4621 ataagtatat actgccctac cgatatattc tgcgaaacac gccacagatg ctccatactt
     4681 gccgccggag cattaagccc ctgcgtgcca tatgccgaaa gcacattgca cagccgcaca
     4741 atatatacaa gtgcaaatgc tgtgacagtg cccaaaaagc caagtatgca tttgattccc
     4801 atatgctgct tcctgccacg gcgggtagtc cttaaaagcc ggatttcatg gtttgcataa
     4861 tccgctccgc atataccgca ggctattgct attaggagaa taacatacac aaaggcctgt
     4921 gctgtatcac ggtttttgaa gtctgttctg tcggtaagaa tatcatagcc cttttcaaaa
     4981 aacagccagc cctcatttaa cgtcttaaga taatccacat gtgctgtaag cttgttgaat
     5041 gcctcctgtc tgctcagttc atccttgtac tttatattga tatagctttc actcttcccc
     5101 tgtgccatat cgtaagcaat gttatctgac agtcggtcgt atattgctct ttcttcatcc
     5161 agaagctcat tggttttggc agttaaaggt ccataatatt tgtccatata ttccttgtaa
     5221 tacacctcat caaccgagtc ataggaaagc ttctctgccg gattccacca taccacaaaa
     5281 atagctgcta caatcaatat aagtaacact tttcccggaa caagtgcctt atagcattcg
     5341 tgcaaaaata aagatgtatg gcagcctgta tttttaccgc aggcaaagcc gggcagtaaa
     5401 agcagcttct tttcatgcac cgaattgaga taatttgtaa ccgcaaaaat acatacagct
     5461 atacacacaa gccagaacaa gccatacaat accccggtat taagcggata tccaaacaga
     5521 ttgatattga cataatcaga aaaaagtctg cctgtcctga ctccgtacat gatatttatg
     5581 tacttaagga aggctaggaa gctggttcct gatatcgttg tgtagcagat agcctcaata
     5641 accacggtca ctgcagaagc aacgaacaca aatataatat tgtcaagcgc tgcgcatatc
     5701 agcataaaaa acaagccaag caatataaag cttattattt tcacaggaaa atagcatgcc
     5761 agaaattgtc ctatactaag cttaaacgga ctgccatagt acagataaac actttgaagc
     5821 ggacttttca gatttatcgg attgtaaaag cacatgctta tgactgcatt gattccatac
     5881 aatagcaagg tgataagaat gaccgccata atcatcacaa atgactttga cgccataagc
     5941 tgccttctgc cccttgcagt tgttctaagc aatgcaagct ggttctcgct tttttcataa
     6001 aacacaagat tgagcgcaat gataaaaagc ataatcacag ccacatagtc ggtaagctca
     6061 ttgtctgtaa tattcaaaag tcccgatgca tcaataacct caggcactgt acctttaagc
     6121 ttactgtaga ctctgctcgt tttagcagca tttttctctg cgaagcctcc attgccggaa
     6181 aacttattca taatcgaaat ctcgtcagcc ttttcattta cagagtcgag atactcatca
     6241 taagacagac atgctcctac ttccttgtat accctgctgc acagactaat cctgccgtaa
     6301 gtttctgccg tcatctgcct ttgttcaatt tccggcaaaa cctcctcacg gctgtaattg
     6361 cttatttcgc catacagcgc gctgtagtcc ttatctgtat acccatcatc atttacggta
     6421 ttcatggtat acagtccaag aacaggatta agaacaagca aaaacaaaag aataagcact
     6481 gttcttttag tcaatagttt acatatttcg ttttttacta tatccatttt cagtccccat
     6541 taagatacaa atacaagtcc tcaagatttg gccgcacagc ctccttctgc catctgccat
     6601 catacgaatc ggcaataatg cgggctatga caccgtctgc atcccttgaa atattgctca
     6661 ccttatatcc ggtgttcacc tcatctatct cattatccga taaaaaaata ttccatacct
     6721 ttccatccag ctcctgaagc agctttgacg gagaaccact ccttattatt ttaccgttgc
     6781 ttaacataac tatctctttg gcgataaact caacatccgt aaccacatga gtcgcaataa
     6841 taacaatctt gtcctttgca acctctgata tcagattgcg gattctgatt ctctcctttg
     6901 ggtcaagccc tgccgttggc tcatccagta taagtatgcc gggattgcct atgagtgcct
     6961 gtgctatcag tgctctctgc ttcattccgc ccgaaaaagc gccaagcttt tttccaagga
     7021 catcatcaag ctttaccatg cgcacatagt gatctatatc ttcccaggct tcactctttt
     7081 ttaatccttt cagggatgcc ataaagtaaa gaaaacgcct aagactaagc tccggataaa
     7141 tattctgctg ctgtggcata tatccgagaa gcttcctgta ttcacttcca agctcgtcta
     7201 tgccccttcc gtcaagcagc acacggcctt ttgatggagt cagattgtca gaaatgagat
     7261 tcatcatagt tgatttaccg gctccattag gtcccaaaag cccatatatg ccgggtgtaa
     7321 atgaaaaaga cacatcatcc agcgcagttt tatccttata aacttttgta agcccctcca
     7381 attcaagtgt cattgtttat attcctccca ctggtttctt acactgtcaa gatagctgtc
     7441 cattccatct gctgtaagct tttcctttgc accttctata ctctcctcaa ccgacttacc
     7501 atttaagcca ttcatgaaat ccgaaagatc atttgagata ttgttcaaat tctccgtatc
     7561 aggctcaaaa ccgataaacg gagatactcc ggcattgtca taaagggcaa aaaactcatc
     7621 ttttcgattt tccatgtatc tttcacctgt taccggatat acatttataa acagatccag
     7681 acagagctta cttaaatagt catcctccag atcagtacca tccgtattgc atgcaacacc
     7741 gtctacgacc ttgtaatccg tgccttcctg tccatacagg agaatatctg cgtactttcc
     7801 gtttccatat aacagaccta aaaactctac cacagcatcg acatccttag tgtttttcgc
     7861 tataccaatg gatgcgttta cccttgatgt cagataatgc tctactgttt ttaccgctat
     7921 attatctttt tggaaattct cgtctatctc tccggaacac aaagctacaa caaaattgcc
     7981 ggacttaaca ttttgtaata tttcctcatt atttaatccg atattattaa ggtatgttat
     8041 ctcaccggtc tcatcgtcct tcaggtatcc gtcttttttc atcttatcga gccccttaag
     8101 atattccgta aacttttgcg attccagtgg attttcgact gacatcgtat cataatcgaa
     8161 gcaaagtccg tttcttatct cacagccgat catatctcca aatacatatt cgtttatgag
     8221 atattgaaat cccggagctt tgctgttatc ccactctgaa gccttgagta tctgataaat
     8281 tccatctatg cttccatccc acgaatttat gacatcatct gagatataat ccctgttaaa
     8341 tgcagcataa actccattat catctgcaag tgccgatggt atggaataaa tatgtccgtc
     8401 acattttgcc atctcccaaa gattctttgg gaaagcatca taaagtgtct ttccctgatc
     8461 tttcgacaaa acatcatcga gatttaagac aagatccgaa cttataaggt catataccgg
     8521 attatttcca ttctcatcgc ctaaacctaa aaatgcaaca tctgtttccc cggcagcaaa
     8581 ggcactttca agcttctggc tgtattcatc atactccaga tattttatat tcagctcata
     8641 gttgtagcca tcactcataa gtgcagcatt aaactgctta aggttattct catcaatcct
     8701 gcaaatatcg ggtacagcaa atgttatgac ctttgtatcc gactttttga ctgttgtctc
     8761 ggtctgtccc tctttaagtt ttcctgaatc gcctgctttt ttattaatac ataccgctgc
     8821 tactgccgcc acgatcaggg caatcactac aactgctatt aaaacaccct ttttcttatt
     8881 cttcattata gcacctcaat tttctcggat taaaattgcc tttattcaga ttatcaagac
     8941 ttataactcc aagtgaaaat tcgccttcat catcagtata gttgacatat accgactgac
     9001 cgcatatgtt ttcaatacca aatgcctttt cagtagacag cttcatcagt tcttcactct
     9061 tatcatcctt caagcggtaa tatgtcactt catcactgtt ttcttcagaa agggcataat
     9121 atagaaaatc tccgctcttt tttaatggtc catattgtgt ttttccatcc gcatctatcg
     9181 gaagctgtct tgtctgtctg ctttcggtgt caaacacaaa atatccatcc tttgacgaat
     9241 aataaccatc gccatctaac agcctaagat tgttaatatg gtctatttcc gtcagacatg
     9301 ttgtcttttc atctgctata tcatattgat atattccatt aagcatatta tcgtaggcaa
     9361 aagactctgc atcagctgtc gccccctcct ctatcatcag ctctgtaaca tcatctccaa
     9421 accgcacagt cgaatagtac actgcaccgt cttcataata tatgcccata atatttgcct
     9481 gttcgcttgt tatcttatca ctcatataca ccttgtaatt atcaagatct ataacaaaaa
     9541 taccggcttc aggcttatca tcatttataa tctgtccttc atcattaagc tccaccctgt
     9601 tgctgtaagc tcctataaca taattatccc tgtacaaaac agccctaaca ttctgaacat
     9661 tctctaattt agctgttttt tttcgattct caccattaga atccatcaca tacagatact
     9721 gtattgtcat gtcttcatct gacatatttc ctataaaata gagatgatca ttgtaaatta
     9781 ccgctccagc ttttgacaat ccataaaaag ctgcagggca tgaaggattc tgatttgtgc
     9841 gtgagtatcc ttcatgtgtg cagtttggct tgtcacaata gacaatatcc ttaccggatg
     9901 ctgtatcaat aagatgcaat attcccgagt catcaaaata gaatacatta tcctcagagt
     9961 agttatattc cggatctaca ttggaatatg tgtacattgt attggatgta ctgcggtttc
    10021 tattctttat tataaaaacc gcagccacaa tacagatgat aagtatgaag atgattgatg
    10081 cgattaatat gcgttttttc ttcatactac cttttccccc ccccaaaaaa aaaaaatata
    10141 tattaattaa catgttgctg atgtctttgt actccatatt tgaccaaaag ctgaaacttt
    10201 atgtgagctt actatcttaa cactatgaca attacttgga gctgaaaact caactgaaca
    10261 atgactataa tgtgcattat tctttgtata tgtccctgta gccagagtat taacatctac
    10321 ataactgtaa gtagatgata cactaacaga tccattataa tcatatgatg tagaggcact
    10381 cgccttttgt tgacgaatag ttgatgaagc tctagttgta tatccattga tagcacctgt
    10441 agccgcaaac actgatatac tcatgcctaa agtcattact aacgttaatg ctaatgctaa
    10501 aactaatttt ttaaaattcg cttttgtgat tttgttcata ttttttcctc atccttttct
    10561 tttaataata ttactaaatt atactgataa aatcaatata ccgctgttga atttgaagac
    10621 catgtctgac catatgcaga aactttatgt gaacttctta ttctaacact atggcaatta
    10681 tatggtgctg aaaaattaat ttcaactgac gtataatagc ctttactttt agtactacta
    10741 cctgtcgcaa gcgtatatgt gttaacataa ctataggttg agtctaccga aatagaacct
    10801 gtcctttttt catatgttgt tgatgcgctt gctgttgtac gagttatatg agatgagcca
    10861 gtaatagcgt atccattaat ggcacctgta gctgcaaata ctgatatact catacctaga
    10921 gtcattacta atgttaaagt gagtactaaa accaatttct taaaattcgc ttttgtgatt
    10981 ttgttcatat tttttctcct tcttctttga tataattaat tatatgtttc tattatttgt
    11041 tacctgaaat cttccttttt caggtctttt aggttgatat aaaatacaag cgcaaaatga
    11101 atgggtttca ttatctcttt ttatctcgaa aataactact tttttgacaa gttctaatgt
    11161 tagtcttttc aaactattca ttttcaaatt tcacctcctg tttaataatt tttgccaacc
    11221 ccaaaagtaa tgcgcataat ataatagaag cagacatata acaaacacat aacctacata
    11281 catcaagcac aataagcaat actattataa tactttctat tgctaaaata catctagcag
    11341 ccttctttga ctcgttaact tcaacataat ccaatgccaa attaggatga tttactaccc
    11401 ctataaaact tattactaat atagagcata taaccagcaa ataaataacc atcatgtaac
    11461 gagctgatat taaacacata tgaataacaa ctatactaat aattattgtt tctatataac
    11521 aatgcaaaaa tgaacttgca tgatatccac cagtcctttt ccttaataat agaaaaaaga
    11581 ataaaaataa catcatttga acagtctgtt taaaaatccc accaacaaat agtactgata
    11641 taatagtaat ccatttttct ataatcatag ctatactata tgtgaaatat tctttcatct
    11701 ctatattcaa taaattttct ttttccattt gttccactat agcattaact atatttttca
    11761 ccatatcttt ttctcccctt ttgtataatt ttatctaaca taatagataa aatcaacaat
    11821 ccatgcacaa aacgaacatt ttttgcagaa aatggacata aactatggtc acatattata
    11881 atgggataat aatagaaaaa ctaaattcat tgttctcatt tttaataaca tattcgccat
    11941 tgtatttttc tacaatttga ataatatttt ttataccaat gccatgcatt tccggttcat
    12001 cttttttact tgtttttatt tcattaccac tgtaaataat tagattactg catgtatttt
    12061 tcaccgaaag aactataaga ttttcctcta tcacaaactt tagttttata gttttctttt
    12121 cctcacattt ttcacatgct tcaatagcat tatttagcag atttgctaaa attactacca
    12181 gatcctcatc ctttattaca atttttgata aatcatttac cttaaacaca aaaacaatat
    12241 tcttactgat agcctcctga tactttgtat taagaatcgc atttattatc acatgatttg
    12301 tattgattgc atttttttct ccaataaaag tattgctgat atttcttaca tatttacttg
    12361 cctcgtcata ttcatgagct ttcaataaag attctataca caatatatga tttttaaatt
    12421 catgtgactt tttcttctga atttcataat tttccgaaat agaacggtac atatcaattt
    12481 ggtttttaaa ctgtagtccc aatacttcct tttcatgcaa tttagctt
//
LOCUS       DPUF01000112            3061 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7672, whole
            genome shotgun sequence.
ACCESSION   DPUF01000112 DPUF01000000
VERSION     DPUF01000112.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7672"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            1..1424
                     /locus_tag="DHV81_04175"
                     /pseudo
     CDS             1..1424
                     /locus_tag="DHV81_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742378.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; frameshifted; too many ambiguous
                     residues; incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
     assembly_gap    861..954
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1263..1361
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1647..2548
                     /locus_tag="DHV81_04180"
                     /pseudo
     CDS             1647..2548
                     /locus_tag="DHV81_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568670.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
     assembly_gap    1766..1858
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2688..>3061
                     /locus_tag="DHV81_04185"
     CDS             2688..>3061
                     /locus_tag="DHV81_04185"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772093.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCI93694.1"
                     /translation="MDEHNHVFDELHEECGVFGMYDFDGGNVASTIYYGLFALQHRGQ
                     ESCGIAVSDTHGPKGKVTTHKGMGLVNEVFTPDILEPMKGDIGVGHVRYSTAGSSTRE
                     NAQPLVLNYVKGTLAMAHNGNL"
BASE COUNT          883 a          500 c          648 g          744 t
ORIGIN      
        1 cttcctgacg gacagatggg aaactctgag gtaggacata tgaatatcgg cgcaggccgt
       61 atcatatatc aggatcttac aagaatcaca aaggcaattg ctgatggtga tttcttcaag
      121 aacaaggtat taatttctgc aatcgaaaac tgcaagaaga atgactccga tctgcatctt
      181 tggggacttc tttcagatgg tggagttcac agccatatcg agcatcttta cggacttctt
      241 gagctttgca agaaagagaa ttttgacaga gtatatgtac acgcattcct tgatggtcgt
      301 gatacaccgc ctgcatcagg taaagactat atcgagcagc ttctcgccaa gatgaaggaa
      361 atcggtgtag gaaagattgc atctctttca ggacgttact atgcaatgga cagagataac
      421 aactgggatc gtgtacagaa ggcttatgat tcactcacta agggtgaagg cgtaaaagct
      481 acatgccctg taaaggctat ggaggagtca tacgcaaatg acgtaacaga tgagttcgtg
      541 cttcctacag ttatcacaaa cgaggatggc acaccggtat ctgttgtaaa ggacaatgat
      601 tctgttatct tcttcaactt ccgtccggac cgtgctcgtg agatgacaag agcattttgc
      661 gatgataagt ttacaggctt tgagcgccca ttccttaaga caacatttgt atgcttcaag
      721 gattatgacg agtcaattcc taacaagctt atcgcttttg agaaggaaga aatcaagaat
      781 acatttggtg agtatcttgc agcaaatggc aagaaacagc ttcgtctcgc agagacagag
      841 aagtatgcac acgtaacctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngttgca
      961 acatatgact taaagcctga gatgagcgct cctgaggttg gaatggatct tgttgaggct
     1021 atcaagtcag ataagtatga tgttattatc atcaactttg ctaaccctga catggttgga
     1081 catacaggtg tgattccggc tgctgtaaag gctgttgaga gagttgattc actcgtaggt
     1141 gatgcagttc aggcaatcaa ggatgtggat ggagtattat tcatctgtgc tgaccacgga
     1201 aacgcagaga agatgattga ttatgagaca ggaaaacctc atacagctca tacaaccaat
     1261 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttgagatt atgggacttc
     1381 cacagccaaa agagatgaca ggaaagtcac ttatcgttaa ataaaatttg tattatatag
     1441 gctccggcgc agacaaaggg tttgaaccgg agcttttgtt ttgatatatt aaagatataa
     1501 taataaatat aagaattgct tataaatagt acaaaattat ttaaaaaaac ttataatata
     1561 cgttgacttt acaaattgag ctggtatata atttaagggt agcatttgat ttgtaaaata
     1621 aattacacat aagatggagg acaatcatgc aggaatttaa accattcaaa gaaggaaaag
     1681 taagagaagt atatgataac ggggacagcc ttattatggt cgcaacagat cgtatttccg
     1741 cttttgacca tattttaaag aataannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     1861 tcctaaccat atgatttcag tagacgcaaa ggatatgccg gagttcttcg gtcaggacag
     1921 gttcaacgga aacagcatgc tctgcaagaa gcttgagatg cttccaatcg agtgtatagt
     1981 tcgtggatat atcacaggaa gcggctgggc aagctaccag gagaacggta cagtctgcgg
     2041 aatcagactt ccggagggac ttgttgagtc tgataagctc cctgagccta tttatacacc
     2101 atctaccaag gctgatcttg gagatcatga tgaaaatatc tcattcgaaa aatcagtaga
     2161 agtattagag aagatttatc ctgagaaggg cagagagtac gcagagaaga ttcgcgatta
     2221 tacaattgct ctctacaaga aatgtgcaga gtatgctctt acaaagggca tcatcatcgc
     2281 agataccaag tttgagtttg gtttaaatga gcagggtgag gtagtgcttg ccgatgagat
     2341 gctcacacct gacagctcac gcttctggcc acttgatgga tataagcctg gacagggaca
     2401 gccttcattt gacaagcagt atgtaagaga ctggctcaag gcaaatccgg acaatgacct
     2461 tcttttacct gatgaggttg ttgtgaagac tgtagagaaa tataaagagg catttgagct
     2521 tcttacagga agcaaattca gccgctaata tattatttat tccgcgttac tatttcgcaa
     2581 ttataaacgg agatattatg aaccaggaca ataacataaa tcagtatata aatactagta
     2641 tagatacaaa aagtcatacc ggtacaaaat taaaaaggtg ttctgatatt gacgagcata
     2701 accatgtatt tgatgagctt catgaggagt gtggtgtgtt cggcatgtat gattttgatg
     2761 gaggaaatgt cgcatccact atctattatg gcctttttgc gcttcagcac agaggacagg
     2821 aaagctgtgg tattgcagtg agtgatacac acggaccaaa gggaaaggtg accacacaca
     2881 agggtatggg ccttgtaaat gaggtattta ctcctgatat tttagagcct atgaagggtg
     2941 atatcggagt gggacatgtg cgctattcaa cagcaggctc atcaacacgt gagaatgcac
     3001 agcctctcgt attaaattat gtcaagggca cacttgccat ggcacacaac ggaaacctca
     3061 t
//
LOCUS       DPUF01000113           13075 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10230, whole
            genome shotgun sequence.
ACCESSION   DPUF01000113 DPUF01000000
VERSION     DPUF01000113.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13075)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10230"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="DHV81_04190"
     CDS             <1..182
                     /locus_tag="DHV81_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93695.1"
                     /translation="PCTLNGFTGARNGQIIPEWSLSGEYVKPKKGAELHKVVNGKDTV
                     VAIFDGKHFVEVKGK"
     gene            186..569
                     /locus_tag="DHV81_04195"
     CDS             186..569
                     /locus_tag="DHV81_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93696.1"
                     /translation="MIKNGEFCVYNGNEYELNEDNDGNLIIITTNREIIDATFVDEYN
                     SGVYSKIISPNEIQEAYECRTYGLINGYKVSIRKELKGTFFVGTGDRKIADILGLNRT
                     DKYLYEKEIAKDKIEVVQEKKKIEV"
     gene            633..1028
                     /locus_tag="DHV81_04200"
     CDS             633..1028
                     /locus_tag="DHV81_04200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93697.1"
                     /translation="MKTLGKIAIYQGTEYEFQKKTDGSYALYSDDIKSLEAGFRRISE
                     KEEKFMKIITLDEVEGVFEKETEVIYGGDTFIGSVIQGSKIMLYTRNVSLGQKYNMTM
                     RDKDEFYKYVKLKDVDCIVQKWTPCEKNV"
     gene            1624..1698
                     /locus_tag="DHV81_04205"
     tRNA            1624..1698
                     /locus_tag="DHV81_04205"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:1657..1659,aa:Glu,seq:ctc)
     gene            complement(1776..2924)
                     /locus_tag="DHV81_04210"
     CDS             complement(1776..2924)
                     /locus_tag="DHV81_04210"
                     /inference="COORDINATES: protein motif:HMM:PF12698.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI93698.1"
                     /translation="MQVFKSYFKILNRHMGQMIMYLSIFIGIMVVLTATRTNKAEGDY
                     EAAKPKFAVFDYDKSSESKALTEYLKKTCIKKNIKNDKKETMQDELFARNVNCVLIIK
                     EGFSLDVVTIPGTQAATTFESAINSFTKTYNTYIASGYSEADALAAVNTSLSQKASVS
                     LLNGKSVTSHSGIFYCFNYLGWVLLVMMIAGIAPVLQVFSKKEIKERTSCSSYKFANY
                     NRELLLGVILTGLAVCAVIFVSATIMFKGSTFSFSGFLFTLNMLCYMLVSLAFAFFIS
                     KITSNSEILNMFANTVSLGMAFLCGIFVPEEFLNDGIIKAAHFLPAYWYNQAVKNIDF
                     HPVKELGFIFMCMGIQLLFAAAIIVVTLRITKAPYGIKKHAVAAAAHK"
     gene            complement(2911..4101)
                     /locus_tag="DHV81_04215"
     CDS             complement(2911..4101)
                     /locus_tag="DHV81_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI93699.1"
                     /translation="MNGLKLFFHNSKYTMLSMFRLKVTLFWTLAFPLLLATFMYMAFG
                     NLFEKDEMFKTINVAVVESENDDTLMSVLESLDVNHTDSASKSDDKADSSNSFSDLIN
                     MKLMDSSAAKKALNDKKVTGIIYTEDASLVVDENSYNATILESILTQYKQQKDVFANI
                     AKTNPAALEAAIAGLSDTTSEYKEISLSSGNQDEYTNYFYAVFAMSCLFASFSAVTAT
                     NRLLANTSPLGMRKSLAALSKNILIFSEYISLLIIHFVVELIALVYLTLLGVDFGNKY
                     PAILLTLFFGCMIGLSIGVIIGAIPKLTEGSKIGISVGIGMVLSVLADLCANGIKDMI
                     EHKLPIANRLNPACLISDCFYSLNVYDSYDRFFRNISIMAVESVILLIIAFALLRREK
                     YASI"
     gene            complement(4101..5048)
                     /locus_tag="DHV81_04220"
     CDS             complement(4101..5048)
                     /locus_tag="DHV81_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93700.1"
                     /translation="MDTTKNAIEVTNLVKRYKELVALDHFNLTVKTGEIFGLLGPNGS
                     GKTTTINCILSLLTYDKGDIKVFGQEMYSNSYDIKREIGVVMQNVAVYDELTVYENID
                     YFCGLYVSDKKLREKYVKEAMDFVDIADYSKFLPKKLSGGLLRRLNIACGIAHKPKLI
                     FFDEPTVAVDPQSRNKILEGIKRLNRDGATIIYTSHYMEEVEQLCDRILIMDKGKALA
                     LGTKDELKRMIKNTETINVEIADLSETQLDSLKQLHNAYQVSFENGQLRIYFSGGRHN
                     IIHVLDYLEQNNLSFGQVYTQLPTLNDVFLEITGKELRD"
     gene            5504..6400
                     /locus_tag="DHV81_04225"
     CDS             5504..6400
                     /locus_tag="DHV81_04225"
                     /inference="COORDINATES: protein motif:HMM:PF07730.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI93701.1"
                     /translation="MLPYAAMVFRKRKYTASRMAAALVVIAVSVDCILKIKHIAPGIF
                     YILEVVLLMAFAVMLSYFTELLLGYQMKLHSMRDASMEHDMLMEQMNHQLIEKQNAQI
                     YNATLKERNRIAREIHDNVGHMITRSILQVGAIGVINTDEKLKAPISDLKSTLDTAMD
                     SMRKSVHDLYDESVDLRQALAKLKPTDSAFAFSLEYDCEDDVPRDVKYAFIAIAKEAV
                     NNAVKHSNGDEIRIIVREHPAFYQLEIMDNGTSADERRLSGETGDGIGIKNIKERVAA
                     IGGTMRIKADDGLMIFVTLMKK"
     gene            6445..7101
                     /locus_tag="DHV81_04230"
     CDS             6445..7101
                     /locus_tag="DHV81_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93702.1"
                     /translation="MKIVIIDDDNLVSLSLNTILSAQDDIEVSAVGDSGQQAVKLYEE
                     HMPDVMLMDIRMQGMTGLEAAEKILGKYPDAKILLLTTFSDDEYIVKAISLGAKGYIL
                     KQAFESIAPALRAVNNGQTVFGTQIMDRIPHLFGADGKASSGTGQARFIDAMKSYDIS
                     GKELSIIELVAEGLNNKEIADELYLSEGTVRNYISVILEKLQLRDRTQLACFYYKSMV
                     "
     gene            7157..7699
                     /locus_tag="DHV81_04235"
     CDS             7157..7699
                     /locus_tag="DHV81_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93703.1"
                     /translation="MINQAQDDFFNELYASTNQKVLAYIIVKCGKTEDVADIFQETYT
                     EVVKIIQKHGISYFKQPDALVMQIAKRKIFRHYKLKEKLRGIEKMNDYNILSADENDI
                     AVQQTSFDDIAISKETVQSVFTYLSAKDELTKKIFYLYYYMDKNISEIALLFSVKEST
                     IKNRLYRTIRELRQNLDKED"
     gene            7706..8239
                     /locus_tag="DHV81_04240"
     CDS             7706..8239
                     /locus_tag="DHV81_04240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93704.1"
                     /translation="MKKNDIFRQAAQHEMPDIEQVRKECIEELHTTHSSKRYPAYFAA
                     IAVVTVVVLCMCITPVRSFAADMIEHIQALIDVNNDSARDLVYRKYSGPTVYLEKDET
                     VSIYSYTGYEDVYILPAEAKEAEKLGAISRDVKVSYKVPYSGEYYILDGENKENITDQ
                     VIVEHEGGAEDNLKISQ"
     gene            8368..8679
                     /locus_tag="DHV81_04245"
     CDS             8368..8679
                     /locus_tag="DHV81_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsoR family transcriptional regulator"
                     /protein_id="HCI93705.1"
                     /translation="MTEKETKCCCCNRKKERSEKEYKDLINRLSRIEGQIRGIKRMLD
                     EDCYCPDIITQVAAANAALNSFNKVLLANHIKTCVADDIRAGKDETIDELLNTLQKLM
                     K"
     gene            8704..9081
                     /locus_tag="DHV81_04250"
     CDS             8704..9081
                     /locus_tag="DHV81_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCI93706.1"
                     /translation="MSTIITILIIAIIAALVIVGLKETIKHSKGEGACCGGGAMKEDE
                     EATVQLTGEIVTRADVHIDGMHCMNCKNSVTRSLQKMSGVSADVDLKHGVAHVEATRE
                     LSDEEITFAIERLDFKVTGIERF"
     gene            9137..>9771
                     /locus_tag="DHV81_04255"
     CDS             9137..>9771
                     /locus_tag="DHV81_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu2+-exporting ATPase"
                     /protein_id="HCI93707.1"
                     /translation="MKQYTVTGMSCAACSARVEKAVSKVDGVTSCSVSLLTNSMGVEG
                     SATDAQIVEAVEQAGYGASPKGTATESENDKANNSLEQLKAAQDALVDRETPKLVNRL
                     IASLIFLVVLMYFSMGHMMWGWPLPEFFNGNHVAMGLLQLLLTVAVMVINQKFFISGF
                     KGLIHGAPNMDTLVALGSAASFGYSVYALFAMTAAQVNGDMDAVMSYMHEF"
     assembly_gap    9772..9971
                     /estimated_length=200
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9972..11795
                     /locus_tag="DHV81_04260"
                     /pseudo
     CDS             9972..11795
                     /locus_tag="DHV81_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569091.1"
                     /note="too many ambiguous residues; incomplete; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu2+-exporting ATPase"
     assembly_gap    10421..10439
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11475..11553
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11817..12995
                     /locus_tag="DHV81_04265"
     CDS             11817..12995
                     /locus_tag="DHV81_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 5"
                     /protein_id="HCI93708.1"
                     /translation="MKKYIALVLAAVLVLGIFTGCGNKGNKETESQTDEKATESVNSA
                     ENNTEDSTQSSTENSTENQNVADTEQLTTENGQESSVLACPSVNGKLHVEGSKLVDQN
                     NNEVQLRGVSTHGLAWYPQYVTNDCFATLKSFGANVVRLAMYTYESGGYCTDGDRQQL
                     ETLVQNGVQYALNNDMYVIIDWHVLNEGNPNRYSDVAKTFFAKMAQQYASYNNVIYEI
                     CNEPCKGATWGDVKFYASEVIPSIRSYDKDAVILIGTPNWSQDVDEAVKDPVTGYDNI
                     MYTLHFYAATHKEDLQNKLKSAADAGLPIFVSEFGICSADGNGQVDIDSANSWISLLD
                     SYGISYVCWNLSNKDEKSALLTPACDTTSGFTYEDLSDEGKWLYGVLTSHVTPETPGI
                     "
BASE COUNT         4009 a         2252 c         3171 g         3345 t
ORIGIN      
        1 ctccttgtac attaaatgga tttacaggtg caaggaatgg gcaaattata cccgagtggt
       61 ctttaagtgg tgaatatgta aaacctaaga aaggtgcgga attacataaa gttgttaatg
      121 gaaaagatac tgttgttgca atatttgatg gtaaacattt tgtagaagtg aaaggaaaat
      181 agaaaatgat aaaaaatggt gagttttgtg tatataatgg aaatgagtac gaattaaatg
      241 aggataatga tggaaactta ataataatta caacaaatcg cgaaataata gatgcaacgt
      301 ttgttgatga atataatagc ggagtatata gtaaaataat ttcgccaaat gaaattcaag
      361 aagcatatga gtgtagaact tatggtttga ttaatggtta taaggttagt attaggaaag
      421 agttgaaagg tacttttttt gtaggcacag gtgatagaaa aattgctgat atacttggat
      481 tgaatagaac agataagtac ttgtatgaga aagaaattgc caaggataaa atagaagtag
      541 ttcaagaaaa aaagaaaata gaagtatgat tgaatcaaaa taatgaatac tgcacgggaa
      601 aacttgaaaa atggtgaata gaaggtaata aaatgaaaac attaggaaaa atagcaatat
      661 atcaaggaac ggaatatgaa tttcaaaaaa agactgatgg gagctatgca ttatactcgg
      721 atgacataaa atcattagaa gcaggcttta gaagaatatc tgaaaaagaa gaaaaattca
      781 tgaaaattat tacattagat gaagtagaag gtgtctttga aaaagaaaca gaggtaattt
      841 atggcggtga tacatttatt ggttctgtta ttcaagggag taaaattatg ctttatacac
      901 gcaatgtgtc gttgggacaa aaatacaata tgacaatgag agataaagat gagttctata
      961 agtatgtaaa attaaaagat gtagattgta ttgttcagaa gtggacgcca tgtgagaaaa
     1021 atgtatgata aaaaatggga aattttgtaa atatcatggg agtgaattta aagtaaatag
     1081 ggatagtgat ggaaatgaga cttccaattt atgatataga agagaaaagg aatatgtagc
     1141 atttggaaaa tacagttgtt aggctactaa atgaaagagg gagaatgagt atcgatttaa
     1201 aaaaaaggtg aaaaatgact aaaacaagca aaaaatgtct aaaaatcaga ttgaaaacta
     1261 attgctcaat gtgcagtttt cacaaagttg ttcccgtgtg accgttgtgt gaccgaaaga
     1321 ttgtataatg ttatctagag gttaagtatg aatagttaaa attaaataaa tggaaggaga
     1381 aatatttgga gtaacaataa attgaggtat caataaacaa gatagatgta ttaaagcttt
     1441 tatgataagc aggtcggaaa gagtaatagt aattatcatt aagcttttac ataagtgaaa
     1501 atgaattaat aaaacaagaa cattaaaggg ataatgttca aggtaaagag atcggaaaaa
     1561 atttaaaaaa aaaatttaaa aaagtacttg cattattcgt ttgaccatga tattataata
     1621 cacggttcgt tggtcaagcg gttaagacgc cgccctctca cggcggaaac acgggttcga
     1681 ttcccgtacg gactgctaag tatttaaagc cggtacattc atggaagcat gatgtaccgg
     1741 ctttttcgtt gtatatattc atatatgagg cagagctatt tatgagcagc tgcagctact
     1801 gcatgttttt tgatgccgta aggcgctttt gtgatgcgaa gggtaacgac aattattgcg
     1861 gcggcaaaca gcagctgtat tcccatgcac atgaaaatga agccaagctc cttgaccgga
     1921 tgaaagtcaa tattttttac agcctggttg taccagtagg caggcaggaa gtgggcggct
     1981 tttatgatgc catcatttag aaattcctcc ggcacaaata taccgcacag gaatgccatg
     2041 ccgagtgaca ctgtgttcgc aaacatgttc agtatctcac tgtttgaggt gattttactt
     2101 atgaaaaagg caaatgcaag tgaaacgagc atgtagcaga gcatgttgag tgtgaacagg
     2161 aaacctgaaa acgagaatgt gctgccttta aacatgattg tagctgagac aaaaattaca
     2221 gcgcacactg caagtccggt taaaatcaca cccaaaagca gctcgcggtt gtagtttgca
     2281 aatttatagg atgagcagga tgttctctcc ttgatttctt ttttggagaa aacctgaagg
     2341 acaggagcta tacctgcaat catcatgaca aggagcaccc atcccagata attgaagcag
     2401 tagaatatcc ctgagtgcga ggtgacactt ttgccgttga gtagcgagac agaagccttt
     2461 tgacttagcg aggtgtttac tgcagctaga gcgtcagctt cagagtagcc ggaagcgatg
     2521 taggtattgt atgtttttgt aaagctgttt atggcagact caaaggtggt cgctgcctgt
     2581 gtgcctggta tggtaacgac atcgagacta aagccttctt ttatgatgag aacacagttt
     2641 acgtttctgg caaaaagctc atcctgcata gtttcctttt tgtcattttt aatatttttt
     2701 ttgatgcatg tctttttaag atactctgtc agagctttgc tttctgatga tttgtcgtag
     2761 tcaaataccg caaatttggg cttggcagcc tcataatcac cttctgcttt gtttgtgcgt
     2821 gtcgcagtga gaacgaccat gatacctatg aatatggaca ggtacataat catctggccc
     2881 atatgtctgt tgagtatttt gaaatatgat ttaaatactt gcatattttt ccctccgaag
     2941 cagtgcaaag gcaatgatta aaagaatcac tgattcaact gccattattg atatatttct
     3001 gaaaaatctg tcgtagctgt catatacgtt gagcgagtaa aagcagtcgg atatgagaca
     3061 ggcagggttg aggcggttag caatcggaag cttgtgctca atcatatcct ttatcccgtt
     3121 ggcgcataaa tccgcaagca cggaaagcac catgccgatt ccgacagaga taccgatttt
     3181 actgccctct gtaagcttag gaatggcacc tattatcact ccgattgaaa gacctatcat
     3241 gcagccgaaa aacagtgtaa ggaggattgc cggatatttg tttccgaagt ccacaccgag
     3301 tagtgtcagg tagactaatg caattagctc gacaacaaaa tggatgatta agagtgagat
     3361 atactctgaa aaaatcagta tgtttttgct tagagctgca aggctttttc gcatgccgag
     3421 aggacttgtg tttgcaagca gacggtttgt ggctgtcaca gctgaaaatg aggcgaacag
     3481 gcagctcatc gcaaatacag cgtaaaagta attggtgtat tcatcctgat ttcctgatga
     3541 aagagaaatc tctttatact ctgatgtggt gtcagatagt ccggcaattg cagcttcaag
     3601 tgcagccgga ttagtctttg ctatgtttgc aaatacgtcc ttttgctgtt tgtactgtgt
     3661 caggatggat tcaagtattg tggcattgta tgagttttca tccactacaa gtgaagcatc
     3721 ctctgtgtaa ataatgccgg tcactttttt gtcatttaga gcttttttgg ctgctgagct
     3781 atccattagc ttcatgttta tgaggtctga aaatgaattt gaactgtcgg ccttgtcgtc
     3841 ggatttactg gctgaatcag tatggtttac atcgagagac tcaagcacgc tcatcagagt
     3901 gtcatcattt tcagattcaa ccactgctac atttattgtc ttaaacattt catccttttc
     3961 aaacagattt ccgaatgcca tatacataaa ggtggcgagc agcagtggaa atgcaagtgt
     4021 ccagaacagc gtgactttca ggcggaacat gcttagcatg gtgtattttg agttgtgaaa
     4081 aaataatttt aatccattca ttaatccctt agctcctttc cggtaatctc taagaatacg
     4141 tcgttaagag tcggaagctg cgtgtacacc tgaccgaagg acagattatt ctgctctaga
     4201 taatcaagca catggattat attgtggcgt ccgccactga aataaatccg aagctgtccg
     4261 ttttcaaagc tgacctgata tgcattgtgc agctgcttta atgagtcaag ctgtgtttct
     4321 gacaggtctg caatttctac gtttattgtc tctgtatttt tgatcatgcg ctttagctca
     4381 tcctttgtgc cgagcgcgag agccttgccc ttgtccatga tgagtattcg gtcacagagc
     4441 tgctcaacct cctccatgta gtgtgaggtg tagatgattg tcgcaccatc acggtttagt
     4501 cttttgatgc cctccagaat cttgtttctg ctttgagggt cgacagccac ggtcggttca
     4561 tcgaagaaga tgagctttgg cttgtgtgct atgccacacg cgatgttgag acgtctcaaa
     4621 agtccgcctg agagcttctt tggcagaaat ttgctgtaat ccgctatgtc tacgaaatcc
     4681 attgcttcct ttacatattt ttcgcggagc tttttgtcac tcacgtaaag accgcagaaa
     4741 tagtcgatat tttcatatac agtcagctcg tcgtacacgg ccacattctg catgaccacg
     4801 ccgatttcgc gtttgatatc gtagctgttt gagtacattt cctgcccgaa tacctttata
     4861 tctcccttgt cgtatgtgag cagggagagt atgcagttta tagttgtggt ctttccggag
     4921 ccgtttggtc cgagcaatcc gaaaatctcg ccggttttaa cggtcagatt gaaatggtcg
     4981 agtgccacaa gctccttgta gcgttttaca aggttggtta cttctatcgc atttttggtt
     5041 gtatccattg aattgcctcc ataatgttgt atttttggtt gcaagcctga tatttggctc
     5101 aataacagat tactatatgg cgtgccggaa agtaagtgtc aaatgtcaca ttcgtattta
     5161 tgtttggatg aggcatggtt gtgtaaaaaa tgacatttgt catagtgacg tttgatggaa
     5221 tttgttgtaa gcttatttta cggaaatgga ggcttttgat gtataagata tgtagtcagt
     5281 ttttaatagt tctatcatgc gtgatgctta tggtggtatc ccttcaggtc gattacgtca
     5341 tgatagcgtg catacttaca ggaataatcg tgtgcacatt tatagacatg caggatgtgc
     5401 cgcagatgag tcctgctaac aagagcaaaa cggcagaccg ggctttggat ttgattctgg
     5461 cggtttatat tctgtccgga gtatttttgc accatcattt tttatgctgc cgtatgcagc
     5521 gatggtattc agaaagagaa aatacacggc atcgaggatg gctgcagcct tagtggtgat
     5581 agctgtatcg gtggactgta ttttaaagat aaaacatata gcacccggga ttttttatat
     5641 cctggaggtt gtacttctca tggcttttgc ggtaatgctt tcgtatttca cagagctgct
     5701 tttggggtat cagatgaagc tgcacagcat gagagatgcg tccatggagc atgatatgct
     5761 tatggagcag atgaatcacc agcttatcga gaagcagaat gcgcagatat acaatgccac
     5821 gctaaaggag agaaaccgta tcgcaagaga gatacacgac aacgtggggc acatgattac
     5881 gcgctctatt ttgcaggtgg gggcaatcgg tgtgataaat accgatgaaa agcttaaagc
     5941 gcctatctca gatctgaaga gtacactcga tacggcaatg gacagcatga gaaaaagcgt
     6001 tcatgatttg tacgatgaat cggtcgattt gcgtcaggcg cttgcgaagc ttaagcctac
     6061 agacagtgca tttgcatttt cactcgaata cgactgtgag gatgatgtgc caagagatgt
     6121 caagtatgct tttattgcaa ttgcaaagga agcggtaaac aacgcagtta agcacagtaa
     6181 cggcgatgaa atcaggataa tagtcagaga gcatcccgcc ttttaccagc ttgagataat
     6241 ggacaatggc acatcggctg acgaaagaag actttcggga gaaacaggag atggaatagg
     6301 cataaaaaat ataaaagagc gtgttgcagc gataggagga accatgcgta taaaggcaga
     6361 cgatggtctc atgatatttg tgacgctcat gaagaagtga ggatatacat aaaaatcaag
     6421 gcttgaaaaa gaaaggaaca ggacatgaaa atagtaataa tagatgatga taatctcgta
     6481 tcactttcgc tgaatacaat actgagcgca caggatgaca tagaggtgtc tgcggttgga
     6541 gacagcgggc agcaggcggt aaagctgtac gaggagcata tgcccgatgt gatgctcatg
     6601 gatataagga tgcagggcat gacaggtctt gaagcagcag aaaaaatact cggaaaatat
     6661 ccggatgcca aaatacttct tctgaccaca ttttcggatg atgaatacat agtaaaagca
     6721 atctcgctgg gggcgaaagg ctatatacta aaacaggcct ttgagagtat tgccccggca
     6781 cttagagctg taaacaacgg acagacagtg tttggaacac agataatgga caggatacca
     6841 cacctgttcg gagctgatgg taaagcttca tcaggcaccg gtcaggcccg gttcattgac
     6901 gcaatgaaga gctatgatat aagcggcaag gagctttcaa tcatagagct ggttgcagaa
     6961 ggactaaaca acaaggaaat agcggatgag ctgtatctga gtgagggaac cgtacgaaac
     7021 tatatcagtg tcatacttga aaagctgcag ctgagggaca ggacacagct tgcatgcttt
     7081 tattataaaa gtatggtata acatgaaact ttttctatgg tgtgggacac atatatagtg
     7141 aagggaggga tgcagattga ttaatcaggc acaggatgac ttttttaatg agctatatgc
     7201 ctcaaccaat cagaaggttt tagcgtacat cattgtaaaa tgtggaaaaa ctgaagatgt
     7261 agcagatata tttcaggaaa cctatacaga ggttgttaaa ataatacaaa aacatggaat
     7321 atcatatttt aagcagccgg atgcacttgt aatgcaaata gcaaagagaa aaatttttcg
     7381 tcattataaa ttaaaagaaa agcttcgtgg cattgagaaa atgaacgatt ataatatttt
     7441 gtcggcagat gaaaatgata tagctgtaca acagacttca tttgacgata tagcaatatc
     7501 aaaagaaaca gtacaaagtg tgtttacata tctttctgcg aaggatgaac ttactaaaaa
     7561 aatattttat ctatattatt atatggataa aaatatttca gaaatagcac tccttttttc
     7621 tgtaaaggaa tcaaccataa aaaacagatt atatcgtacc ataagggagc ttaggcaaaa
     7681 tctggataag gaggattgat gcagaatgaa aaaaaatgat atttttagac aggcagcgca
     7741 gcatgaaatg cctgatattg agcaggtaag aaaagaatgt atcgaggaat tacataccac
     7801 tcacagctca aaacgatatc ctgcatattt tgctgcgata gcggttgtga ctgtagttgt
     7861 cttgtgtatg tgtatcacgc cggtgaggtc atttgcagcc gatatgattg aacatattca
     7921 ggcattaatt gatgttaata atgattcggc acgtgatctg gtgtatagaa aatatagcgg
     7981 tccgactgtt tatttggaaa aagatgaaac ggtaagcata tactcatata ccggttatga
     8041 ggatgtatat attttgccgg cggaggcaaa agaagctgaa aaactgggag ctataagcag
     8101 agacgttaag gtgagctata aagtgccgta tagtggggag tattatattc ttgatggaga
     8161 aaataaggag aatatcacag accaggttat tgtagaacat gagggaggag cagaagataa
     8221 tttgaaaatt tctcaataag gaaatctgcc cttgcaatat accccactga ggtatataat
     8281 gctcttgaag acagatacct gtaaggggta tggttagata tgtgaaacaa atgcataatg
     8341 cggaataaag aacaaatgga ggtacacatg acagaaaaag aaaccaaatg ctgttgctgc
     8401 aaccgcaaaa aagagcggtc agagaaggaa tataaggatc tcatcaacag acttagccgt
     8461 atagaggggc agatccgtgg aatcaagcgt atgctggatg aggattgcta ctgccctgac
     8521 atcatcacac aggtggcggc cgcaaatgct gcgctcaaca gcttcaataa agtccttttg
     8581 gccaatcata taaagacgtg tgtggcagat gacataagag ccggcaagga tgagacaata
     8641 gatgagcttt tgaacacact gcagaagctt atgaagtagt attataagaa ggcgggtata
     8701 aaaatgagta caataatcac aatattaata atagcaataa ttgccgcact ggtgattgtg
     8761 ggcttaaagg agactataaa gcattcaaag ggcgagggcg cctgctgtgg cggtggagcc
     8821 atgaaggagg acgaggaagc cacagtacag cttacaggcg agattgtcac gagagcagat
     8881 gtgcacatag acggaatgca ctgcatgaac tgcaagaact cagtcacacg ctcacttcag
     8941 aagatgagcg gagtatcggc agatgtggat ttaaagcatg gcgtggcaca tgttgaggca
     9001 accagagagc taagcgatga ggaaatcaca tttgccatcg aaagactgga tttcaaggtg
     9061 acaggaattg agagatttta gaaaaggctt ttgaatagtt agattcaaat agtagaaaga
     9121 aggaaataca gacagtatga agcaatatac agttacagga atgagctgtg cggcatgctc
     9181 tgccagagtg gaaaaggctg tctctaaggt tgatggtgtg acgagctgct ctgtcagcct
     9241 tttgaccaac tcgatgggag tcgagggaag cgcgacagat gcgcagatag tggaggcagt
     9301 agagcaggcg ggctatggag cgagtcccaa aggcacagca acagaatcag aaaatgacaa
     9361 agcgaataac agcttagagc agctaaaggc agcgcaggat gcgcttgtag acagggagac
     9421 accgaagctt gtaaacaggc ttatagcatc gctcatattc ttagttgttc tcatgtattt
     9481 ttccatggga catatgatgt ggggctggcc gttgcctgag ttcttcaatg gaaaccatgt
     9541 ggctatgggg cttttgcagc ttctgcttac agtggcggta atggtcataa accagaagtt
     9601 ttttatcagt ggctttaagg gcttgattca cggcgctccc aacatggata cgcttgttgc
     9661 gcttggctct gcggcatcgt ttggatacag cgtgtatgca ctttttgcca tgacagctgc
     9721 gcaggtgaat ggcgatatgg atgcggtcat gtcatatatg catgagtttt annnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn ngtggacggt gaaataatag atggaacaac cgctgtcaac gaatcggcac
    10021 tcacaggaga gagcatccct gtggataagc agccgaaaga tgcagtaagc gctgccacag
    10081 tgaaccagtc aggctttata aaatgccgcg caaccagagt aggcgaagac accacactct
    10141 cacagattat acagatggtt tctgatgccg cagcgacaaa ggcacctatt gcaaaaattg
    10201 ctgacagggt atcgggagta tttgtgccgg cggttattac aattgcaatc attacaataa
    10261 tagcatggct tatagccggt gagaccgtgg gctttgcgct tgccagaggc atttcggtgc
    10321 tcgtgataag ctgtccgtgt gcacttggac tggcaactcc tgttgcgata atggtaggaa
    10381 acggcaaggg cgcaaagagc ggtatactct tcaagacagc nnnnnnnnnn nnnnnnnnng
    10441 caagccttga ggcaacaggc cgcacacaga ttgtggctct tgataagaca ggcaccataa
    10501 caagcggtga gccgaaggtg acggatatcg tgccggatga gacatttttt gaggaaaccg
    10561 gcaagcatgc cggtgagtgt cagcgcaagg tagacgattt aaacccgtac tcatcaacag
    10621 ataaagcact gtggtcaagg aagcttcttg caatagccgc atcagtcgag gccaagagcg
    10681 agcatccgct tgcaaaagcc attatggagc gcgctaaaac ggatgaaatc gctgttgcag
    10741 aggtgacgga ttttagcgct gttgtaggaa atggtctgac tgcaatactt gcaggcaaaa
    10801 tgataaaagc aggaaatctt gcttttgtca gcaaatttgt aaaagtgtca gatgatatgc
    10861 gcacgaaagc tgtcgaattt tcaaaggaag gaaaaactcc tctgttcttt gccgcggatg
    10921 acaggctttg cggtatcata gccgttgcag atacaataaa ggaggacagt ccggaagctg
    10981 tcagacagct taaaaatatg ggtatcagag ttgttatgct caccggtgac aacgagcaga
    11041 ctgccaatgc cattggaaag caggcgggag tggacgaagt catcgcaggt gtgcttccgg
    11101 atggtaagga ggctgtcata aggaagctaa aaaaacaggg cagggtagcc atggttggtg
    11161 acggaatcaa tgatgcaccg gcgctcacac gtgcggatat gggaattgca atcggcgcgg
    11221 gaagtgatgt ggcaatagat gcagcggatg ttgtcctgat gaagagccgc ctgatagatg
    11281 taccggcagc agtgcggtta agccgtgcta cattgacaaa tatacacgaa aatctgttct
    11341 gggcattttt ctataatgta ataggtattc cacttgcggc cggtttgtgg tatcctttat
    11401 taggatggaa gctcaatccg atgtttggag cggcggcgat gagcctgtcg agcttctgcg
    11461 ttgttaccaa tgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaaataa accaaatgaa agtgaggaaa
    11581 aatcaatgac aaagacaatg aatatcgaag gaatgatgtg cggccactgc gaggcaagag
    11641 taaagaaggc actcgaggcg cttgatgcag ttagtgaggc agcagtaagc catgagagcg
    11701 gaactgcagt agtgacatta agctctgaca tttccgatga gaaattaaag gagactgtag
    11761 aggctgagga ctacaaggtt acatcaattc agtaatcagt cagacagtat aacaatatga
    11821 agaaatatat agcattggta ttggctgcag tgctggtgct tgggattttt acaggatgtg
    11881 gtaacaaggg caacaaagag acagaatcac agacagatga gaaagccact gagtcggtga
    11941 attctgcaga aaataatact gaggacagta cgcaaagcag tacagaaaac agtacagaaa
    12001 atcagaatgt ggctgacaca gagcagctca ccacagaaaa cggacaggag agctcagttc
    12061 ttgcttgtcc gtcagtaaac ggtaagctgc atgtggaggg cagcaagctc gttgatcaaa
    12121 ataacaacga ggtgcagctt cgcggagtca gcacacacgg tcttgcgtgg tatccacagt
    12181 atgtgaccaa tgattgcttt gccacgctga aaagctttgg agcaaacgtg gtgaggctcg
    12241 ccatgtacac ctacgaaagc ggtggctact gcacagatgg tgacaggcag cagcttgaga
    12301 cacttgtgca aaacggcgtg cagtacgcac taaacaacga catgtatgtc atcatagact
    12361 ggcatgtgct aaacgagggc aatccaaacc gctacagtga tgtggcaaag acattttttg
    12421 ccaaaatggc acagcagtat gcctcataca acaacgtgat ttacgagatt tgcaatgagc
    12481 cgtgcaaagg tgcgacatgg ggagatgtga agttttacgc cagcgaggtc attccgtcaa
    12541 tcagaagcta cgacaaggat gcagtcatac tgataggcac acctaactgg agccaggatg
    12601 tggatgaggc agtcaaggac cctgtcacag gctatgataa tatcatgtat acactgcatt
    12661 tctatgccgc aacccacaag gaggacttgc aaaacaagct aaaatcggcg gcagacgccg
    12721 gactgccgat atttgtatca gagtttggca tctgtagcgc tgacggaaac ggtcaggtgg
    12781 acatagattc cgcaaacagc tggatcagcc tgctcgacag ctacggcatc agctatgtgt
    12841 gctggaatct gtccaataag gacgaaaaat cagcgcttct aacacctgcc tgcgatacga
    12901 caagcggctt tacctatgag gatctaagcg atgagggcaa gtggctgtac ggtgtgctca
    12961 caagtcatgt gacaccggaa acacctggca tataaaaaca ttcggaggta atatacactc
    13021 agccggcatg cacatgaggc agatgcagat atttctctct ggtggcaagt ccgcc
//
LOCUS       DPUF01000114            6676 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10237, whole
            genome shotgun sequence.
ACCESSION   DPUF01000114 DPUF01000000
VERSION     DPUF01000114.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10237"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="DHV81_04270"
     CDS             <1..318
                     /locus_tag="DHV81_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCI93709.1"
                     /translation="YLEKMIKTAVVISDESKDDEVGMFNTVDVYFEDDDETETYKIVT
                     TVRGNSLKNLISKESPLGAAIFGHKVGDRCEVKVNDDYSYFVKIMKIQNTVDDGSDDL
                     RKF"
     gene            412..1629
                     /locus_tag="DHV81_04275"
     CDS             412..1629
                     /locus_tag="DHV81_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI93710.1"
                     /translation="MIKLKTFGSFTVGNGLTSVQEGAWRSEKLEKLFVYLAMNRNRGV
                     CIEDISEAIWQVEEETDNPVGALKNLAYRLRKALRDASFGEECIVSVRGGLYKWNDDV
                     EVTIDVEEFDRYINEAEMAFCRSKETAIESYEKAIALYHGDFLPLRTDTHWFMTLNAF
                     YHSRYVNTVKALAKLYIDTGCYEKLEQLCIRAIVYERSDEQIYSYLIMARMRTKKVQM
                     AFDTYETAKAIMDKDLGVRKTVMLNKVYEELLSVTKGVSSYNIDEVKEDINEESMEGV
                     FMCGYPVFKEIYHLEVRKSARSTVPESLVLITVAPMFNTLADKEHSRMKDSMHTLERA
                     LRKSLRVGDVAAKYSDSQYIVLLSKCSCDTASDVMKRIIDRFNHTCDTHSNMTIHFDI
                     EPVSCYSSFVSDK"
     gene            1655..2134
                     /locus_tag="DHV81_04280"
     CDS             1655..2134
                     /locus_tag="DHV81_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCI93711.1"
                     /translation="MRRLINIIFGVILLILVLIIAVIFAPKLFSIEPMVVVSGSMEPS
                     YMKGSLLYVKEDTGGIQTGDAITFYRNGELVTHRVVEINTDEKTYTTKGDANQVNDVQ
                     PVAWSDVVGVPVFDVPVLGYPASFLGTSQGKFVFIVLLIIFTGITVLTDKKMEKTNG"
     gene            2127..2900
                     /gene="srtB"
                     /locus_tag="DHV81_04285"
     CDS             2127..2900
                     /gene="srtB"
                     /locus_tag="DHV81_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB family sortase"
                     /protein_id="HCI93712.1"
                     /translation="MVKKVIRFIVLIIGLSLVAYSGYHLFKIYSDYNTSDKTYEKLQD
                     EYAVDDSKKDDDSTKGSEAQSPWYDDIDIDFAGLRSENPDVVGWIYFENEDISYPVMY
                     SGDNSYYLRKTFKREHATAGSIFLEGSNKTDFSDCHTIIYGHNMKNLSMFGKLKYYNR
                     DENYYDSHQYFQILVDGKKYRYQIFSYETVSDDSDEYTVGFEPDETFGKFVQRMAASS
                     MKDTGIVPTKDDKVVTLSTCSTSGETYRFVVHGVRVDEH"
     gene            2922..3374
                     /locus_tag="DHV81_04290"
     CDS             2922..3374
                     /locus_tag="DHV81_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93713.1"
                     /translation="MKKEDELLKELTDMVKETKKGQIKWKLSCQTTEYNDEAVKPTVT
                     EDGITWTVDECYVSYECTYKGSDFVMITYEMIHTAGDKRQTTNLVFLPPLGIRYFDIS
                     TLLPYSVPASNILTYEIHTLWLLLLDMYKSDNTSVELDASSRELMIEE"
     gene            3388..4164
                     /locus_tag="DHV81_04295"
     CDS             3388..4164
                     /locus_tag="DHV81_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCI93714.1"
                     /translation="MYAVITGASSGIGEQFAKRLAKEGYDLILVARRRERLTALSDKL
                     KATHKELECDIFTADLMQLDECQRLSDYLEEKDIEIFINNAGYGDCGLFEETDIAKEM
                     GMIDVNVRAVHFLTKKLLRQMRVKDRGFILNVASSAGLIPAGPYMAAYYASKAYVASL
                     SRAVACELRQSHSHVYVGCLCPGPVDTEFNDVANVRFALKGISAEYCANYAIDRMMKH
                     KTVIVPTLRMKLATTCGRFLPQSLYIKIVSHQQKKKWHID"
     gene            4256..6079
                     /gene="pulA"
                     /locus_tag="DHV81_04300"
     CDS             4256..6079
                     /gene="pulA"
                     /locus_tag="DHV81_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HCI93715.1"
                     /translation="MKLGAIYSKEKTTFTLFSPVADSVFVIFYDKGNDSAEKGRLEMM
                     RTEGELRSIFSATAEGNLDGVYYTYEVHTSDGTFSSHDPYARACGVNGHRSMVIDLSK
                     TDPDGFADEDRPKLADPMSMVITEVSVADVSAGASAHSRYPGKFKAFTEDKTLSYFKD
                     MGVTHLQLMPSYDFASIDESDSLKEQYNWGYDPYNYNVPEGSYSTDPFNGAVRVREYK
                     EMVHSIHEAGLGVIMDVVYNHTFNVHDSCFYKTAGNYFYRMLDAAKYSDASACGNEIA
                     SEKPMVRKYIIDSLCYWASEYHIDGFRFDLMGVLDIETLNEARKALLKINPDIIMYGE
                     GWTGGESTLPESERGMKINAPRTPGIGMFSDDIRDAVKGHVFYMDELGFVNGAADRGE
                     ELKQAVTCAKWAKSPMQSINYLSCHDNYTLWDRFIISNSHESEEMRIRMNKLAAAILF
                     TAQGIPFFLHGEEFARTKISEADGTPVENSYCSPLSVNAIDYDRAEQFSDLKAYYKGL
                     IALRRAHKSFYLDTVDEIKKSVHFMDDMPDGVVAYTIDNDTEKVFVAYNAGKNKITIK
                     LADALWEVLADEASAGDKALYTIKDQAIIPGVSCLVAVCKC"
     gene            complement(6076..>6676)
                     /locus_tag="DHV81_04305"
     CDS             complement(6076..>6676)
                     /locus_tag="DHV81_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HCI93716.1"
                     /translation="LNSVKISVLAILCALVSILFCVALHSSEALYKKYLPNKYTRIFI
                     GGCFVIIATMLVGNQTYNGTGMSVIQSSIDGSVRPEAFLLKIIFTALTLGAGFKGGEI
                     VPSFFIGATFGCLFGNLTGFEPSLCAAVGMISLFCGVTNCPITSLLISFELFGYDGMP
                     YFLLAIPFSYMLSGYFGLYRSQKIVYSKYKTSYINKSTH"
BASE COUNT         1947 a         1152 c         1717 g         1860 t
ORIGIN      
        1 taccttgaga agatgattaa gactgccgtt gtcatatccg atgagtcaaa ggatgatgag
       61 gtgggtatgt tcaatacggt tgatgtgtat tttgaggatg atgatgagac tgagacatac
      121 aagattgtca caacagtaag gggcaattcc ttaaagaatc tgataagcaa ggagtcaccg
      181 cttggtgcag ctatattcgg gcacaaggtg ggtgacaggt gtgaggtaaa ggtaaatgat
      241 gactactcat actttgttaa aataatgaaa atacaaaata ctgtcgacga cggcagtgat
      301 gatttgcgta aattttaatc tgggtttaat ttttgagtct tatagggatt ccaaaaagat
      361 taatatgttt atgactatat aatgtttaat gtcagaaatg gggagattta tatgattaaa
      421 ttaaaaactt ttggcagctt tacggtaggt aatggcttaa catctgtcca ggaaggggca
      481 tggcggtctg agaagcttga aaagctgttt gtatatctgg cgatgaacag aaacaggggt
      541 gtctgtatag aggatatttc ggaggctata tggcaggttg aggaggagac tgataatccg
      601 gtaggagccc ttaagaatct ggcatacagg ctcagaaagg ctctaagaga tgcctccttt
      661 ggcgaggagt gcatagtctc tgttagagga ggcttgtaca aatggaatga tgatgtggaa
      721 gtaaccattg atgtcgaaga gtttgacaga tatataaatg aggctgagat ggcattttgc
      781 aggagcaagg agaccgctat tgaaagctat gagaaggcca tagcccttta ccatggagat
      841 ttcctgcctc ttaggacaga tacacactgg tttatgacgc tcaatgcatt ttaccattcc
      901 agatatgtaa atacagtaaa ggctctggca aagctgtata ttgatacagg ctgctatgag
      961 aagcttgagc agctgtgcat cagggcgatt gtatatgagc gctcggatga gcagatatac
     1021 tcatatctta ttatggcacg catgcgtacc aaaaaggtac agatggcttt tgatacttat
     1081 gagactgcca aggctattat ggacaaggat cttggcgtta gaaagactgt catgctcaac
     1141 aaggtgtatg aggagctttt atctgtcaca aagggtgttt caagctacaa tatagatgag
     1201 gtgaaggagg atatcaacga ggagagcatg gagggcgttt tcatgtgcgg atatcctgta
     1261 ttcaaggaga tttatcatct ggaggtcagg aagagtgcgc gctccactgt tccggagagt
     1321 cttgtgttga ttactgtggc accgatgttt aacactttag ctgataagga gcatagccgg
     1381 atgaaggatt ccatgcatac acttgaaaga gcacttagaa agagcctgcg tgtgggtgat
     1441 gtggcagcaa agtacagtga ttcacagtac attgtgcttt tgtcgaagtg ctcatgcgat
     1501 acagcctccg atgtcatgaa gcgtattatc gataggttca atcatacgtg tgacacacat
     1561 agtaatatga caatacactt tgacattgag ccggtaagct gctacagcag ttttgtgagt
     1621 gacaaatgat attttctgat atagaggtgt tttcatgagg agacttatta atattatttt
     1681 tggtgtaatt ttattaattc ttgttttaat tattgctgta atatttgctc ctaagctgtt
     1741 tagtatcgag cctatggtag ttgtaagcgg aagcatggag ccttcttaca tgaaggggag
     1801 cctattgtat gtaaaggagg ataccggtgg gatacagaca ggtgatgcca ttacctttta
     1861 cagaaatggc gagcttgtga ctcacagggt agttgaaata aatacagatg aaaagaccta
     1921 cactacaaag ggagatgcca atcaggtgaa tgatgtacag ccggttgcat ggtctgatgt
     1981 ggtcggagtg cctgtgtttg atgtcccggt gctggggtat cctgcttctt ttttaggaac
     2041 ctcgcaaggt aaatttgtat tcattgtatt gttgattatt tttaccggta ttacagtact
     2101 gactgataaa aaaatggaga aaacaaatgg ttaagaaagt aattagattt atagttctga
     2161 taattggtct gtctcttgtg gcttattcgg gttatcatct gtttaagatt tattctgact
     2221 ataatacctc ggataagaca tatgagaagc ttcaggatga gtatgcagtg gacgattcaa
     2281 agaaggatga tgacagcacg aaaggcagtg aagcacagtc gccgtggtat gatgatatag
     2341 atattgattt cgccggtcta aggagcgaaa accctgatgt ggttggctgg atttattttg
     2401 agaatgagga tatcagctac cctgtcatgt attcggggga taatagctat tacctcagaa
     2461 agacctttaa gagagagcat gccactgcag gctccatctt ccttgagggt tcaaacaaga
     2521 ctgatttcag cgactgccac acaatcattt atggacacaa tatgaaaaac ctgtcgatgt
     2581 ttggaaagct taagtactat aaccgtgatg agaattacta tgacagccat caatatttcc
     2641 agatacttgt cgatggcaaa aagtataggt accaaatttt ctcatatgaa acggtaagtg
     2701 atgacagtga tgagtatact gtgggctttg agcctgacga gacatttggt aaattcgtac
     2761 agaggatggc tgcctcatca atgaaggata caggcatagt cccgacaaag gatgacaagg
     2821 ttgtgacgct ttcaacctgt tccacaagcg gtgagacata caggtttgtg gtacatggtg
     2881 tgagggttga tgagcattga taatgaaaac aggtgtataa tatgaaaaaa gaagatgaat
     2941 tacttaaaga gcttaccgat atggtaaagg aaacaaagaa ggggcagatt aagtggaagc
     3001 tttcctgtca gactacagag tacaatgatg aggccgtaaa gccgacagtt acagaggatg
     3061 gcatcacctg gactgttgat gagtgctacg tgtcatatga gtgtacctat aagggcagtg
     3121 attttgtgat gattacatat gagatgattc atactgccgg tgataaaaga cagacaacca
     3181 accttgtttt tttaccgccg cttggtatca gatactttga tatcagcaca ctgcttccat
     3241 actcggtgcc tgcatccaat attctcacct acgagattca cacgctgtgg ctgttgctgc
     3301 ttgatatgta taagagcgac aatacaagcg tagagcttga tgcgagctcc agggagctta
     3361 tgattgaaga atagaggggt atgatatatg tatgcagtga ttacaggagc aagctcagga
     3421 ataggtgagc agtttgcaaa gagacttgcg aaggaggggt atgacctgat tcttgttgcc
     3481 agaaggcggg agagacttac agctctttct gataagctta aagccacaca taaggagctt
     3541 gagtgtgata tttttaccgc ggatctgatg cagcttgatg agtgtcagag actttcggac
     3601 tatctcgagg aaaaagacat agaaatattt ataaacaacg caggctacgg tgattgcggc
     3661 ctgtttgagg agactgatat agctaaggaa atgggcatga tagatgtcaa cgtgcgtgct
     3721 gtacacttcc tgacgaaaaa gcttttaaga cagatgaggg taaaggacag aggttttatc
     3781 ttaaatgtgg caagctctgc gggtcttatt cctgctggtc cgtacatggc tgcctattat
     3841 gcctctaagg catatgttgc gagcctttca agggctgttg catgtgagct tcgacagagc
     3901 cacagccatg tgtatgtggg gtgtctgtgt ccgggaccgg ttgatacaga gttcaatgat
     3961 gtggcgaatg tgcgatttgc gctgaagggg atttccgctg aatactgcgc caattatgcc
     4021 atagaccgga tgatgaagca caaaacggtc atagtgccta ctctcaggat gaagcttgct
     4081 acaacctgtg gcagatttct gccacaatct ttgtatataa agattgtttc acatcagcaa
     4141 aagaaaaaat ggcacattga ctaaaaagtt tttgaaaatt ggttgaattg ccctatttac
     4201 agttattgta aattttatta taatgtatac gaaaataaac gaaaaggata gtgctatgaa
     4261 attaggagcg atttattcaa aggaaaagac aacatttaca ctgttttcgc ctgttgcgga
     4321 cagtgttttt gttatttttt atgataaggg aaatgattcg gccgagaaag gcaggcttga
     4381 gatgatgcgc acagagggag agcttcgcag tattttttct gctacggcag aggggaatct
     4441 ggatggtgtt tattatacat atgaggttca cacctcagat ggcacattca gctcgcatga
     4501 tccgtatgca agagcctgcg gtgtaaatgg gcacaggtct atggttattg acctgtcaaa
     4561 gactgatcct gatggatttg cagatgagga caggccaaag cttgcagacc ccatgagcat
     4621 ggttatcact gaggtgtctg tcgcggatgt gtctgccggt gcatctgctc attccaggta
     4681 tccgggtaag tttaaggcat ttactgagga taagactctt tcgtacttca aggatatggg
     4741 agtgacacat ctgcagctta tgccgtcgta tgattttgcc agcattgacg agagtgattc
     4801 cttaaaggag cagtataact ggggctatga tccgtacaat tacaatgtgc cggagggctc
     4861 ttactcgaca gatccgttta atggagccgt gagagtgaga gagtataagg agatggtaca
     4921 ctcgattcat gaggcgggtc ttggtgtaat catggatgtg gtttacaatc ataccttcaa
     4981 tgttcatgac agctgctttt acaagactgc gggcaattat ttctaccgca tgctggatgc
     5041 tgcaaagtac tctgatgcat cagcctgtgg caatgaaata gcctcagaga agcctatggt
     5101 cagaaaatat atcattgatt cgctgtgcta ctgggcgagt gagtatcaca ttgatggctt
     5161 caggtttgat ctgatgggag tacttgatat cgagacattg aacgaagcca gaaaagctct
     5221 cttaaaaata aatcctgata taataatgta cggagagggc tggacaggtg gagagagcac
     5281 tctgccggaa tcagagcgtg gcatgaaaat aaatgctcca aggacaccgg gcatcggcat
     5341 gttttcggat gatatacgtg atgcggtaaa gggacatgtg ttctatatgg atgagctggg
     5401 ctttgtcaat ggagctgcag acagaggtga agagctaaag caggctgtta cgtgcgctaa
     5461 gtgggccaaa tcgcctatgc agtcgattaa ctatctgtca tgtcacgaca attatactct
     5521 ctgggacagg tttattatat ccaattcaca tgagagtgaa gagatgcgta tccgtatgaa
     5581 taagcttgca gcagccatcc tttttacagc gcaggggatt ccgtttttcc tgcatggaga
     5641 ggagtttgcg agaacaaaga ttagtgaggc tgatggtact cctgtagaga acagctattg
     5701 cagtcctctc tctgtcaatg caatagatta tgacagggct gagcagtttt cggaccttaa
     5761 agcctactat aaggggctta tagcactaag aagggctcac aagtcatttt atcttgacac
     5821 agtagatgag attaagaaat ccgtacattt catggatgat atgcctgatg gcgttgtagc
     5881 atatactatt gataacgata cagagaaggt gttcgtagct tacaatgccg gcaaaaataa
     5941 aatcacaatt aaacttgcag acgctttgtg ggaggttctc gcagatgaag cttctgcggg
     6001 agataaagct ctttacacaa taaaagacca ggcaataata cctggggtat catgcctggt
     6061 tgcagtttgc aaatgttagt gagttgattt atttatatag cttgtcttgt attttgaata
     6121 tacgattttc tggctgcgat acaggccaaa gtatcctgac agcatatagc tgaatggaat
     6181 tgccaaaaga aagtacggca tgccgtcgta gccaaacagc tcaaagctga tgagcagcga
     6241 tgtgatcgga cagttggtga caccacagaa cagtgaaatc atgccgacag ctgcacacag
     6301 cgatggctca aagcctgtca gattgccgaa caggcatcca aaggttgcac ctatgaaaaa
     6361 ggatggtaca atctcgccac ccttaaagcc tgcgccaaga gtcagtgcgg tgaagatgat
     6421 ttttagcaga aatgcttcag gtctgacaga accgtctata ctgctttgga tgacgctcat
     6481 tccggtgccg ttgtaggtct ggtttcctac aagcatagtt gctatgatga caaagcagcc
     6541 accaatgaat attcttgtat atttgtttgg cagatatttt ttgtacagcg cctcacttga
     6601 gtgaagggcc acacaaaaga gtatgcttac gagtgcgcac agtattgcaa gcacagagat
     6661 ttttactgag tttaaa
//
LOCUS       DPUF01000115            8159 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6192, whole
            genome shotgun sequence.
ACCESSION   DPUF01000115 DPUF01000000
VERSION     DPUF01000115.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6192"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     assembly_gap    153..296
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <297..509
                     /locus_tag="DHV81_04310"
     CDS             <297..509
                     /locus_tag="DHV81_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93717.1"
                     /translation="STPRYSQAFKSVGQAVSFDNIQPGDVVVYPGHVAIYAGGGVIVE
                     AQSTKAGITANRSVQCHTILAIRRLV"
     gene            963..1403
                     /locus_tag="DHV81_04315"
     CDS             963..1403
                     /locus_tag="DHV81_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCI93718.1"
                     /translation="MQMEQLQKDMIAAMKARDKVRKDAISSLVSAAKKAAIDEGCRDN
                     IPEDLVNKVILKEMKSVKEQIDTCPATRTDLLDEYNTRYAIFEEYAPAMMSAEEVEKE
                     LTEQFSDVIATGNKGQIMKAVMPHFKGKADGKVINQVVAKLCAK"
     gene            1391..2053
                     /locus_tag="DHV81_04320"
     CDS             1391..2053
                     /locus_tag="DHV81_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93719.1"
                     /translation="MRKIRKHITTIILAVLAVIAGVYAYNYHDMKQNIVYNEHLEDVA
                     VNVNGKELTLRDMAFYVAYEEMNVEKQALVYDSDNPNKYWNIHTNGEFVRVAARKAAM
                     SMAIHDEIFYEMAKKESITLTDDEKAALKNSEKDFWYDLSDIDGAKKLGVEKKDIYSS
                     MEKSAIARKYQEIYAGLDNADITDYDFSGGRYKKLLEKNNYKIKEKVWKRVDMGNVTL
                     DH"
     gene            2066..2941
                     /locus_tag="DHV81_04325"
     CDS             2066..2941
                     /locus_tag="DHV81_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HCI93720.1"
                     /translation="MSTKIGRNDPCWCGSGKKYKTCHAAFDMKLEKLAEAHHKIPSHS
                     IIKTPDQIAGIKESAKINVAVLDYIGEHIHEGMNTAEIDKIVYDMTTSMGGIPAPLNY
                     EGYPYSVCTSVNEQVCHGFPSKDVILKDGDIINVDCSTILNGYFSDSSRMYCIGNVSP
                     EKKKLVEVTKECVELGLKEVKPWGFLGDMGQAVHDHAFANGYTVVREIGGHGVGLEFH
                     EDPWVGYNTKRGTDMVMAPGMIFTIEPMVNMGKADIYVDDKNNWEVYTDDGLPSAQWE
                     IMVLVTEDGHEVLSW"
     gene            complement(3372..4700)
                     /locus_tag="DHV81_04330"
     CDS             complement(3372..4700)
                     /locus_tag="DHV81_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93721.1"
                     /translation="MNKKEVLELKRRLKKDQATFSRLVGCYVDCNHEKVCKFGGKFLT
                     LEEDEYYKYLEISNKVLSGTLGNNLLNLQFPLEEEEVGGRQQILMALRDSELNDETLL
                     DTYYDLVIDTYDEAGNYLILLYLDSYDVMTRTKDNINVDESEEVYKYMLCAICPVTLS
                     KPGLGYLEKEQRIGARIRDWVVGAPETGFLFPAFNDRSTDIHSTLFYTKNTKEPHSEF
                     MTNGLGCGVERTATEQKMAFHSIVRNVLGAEDESTDDKLLDIQQNLSEMADDYAETHD
                     DDDDPFILDSEAMNKVLSDCHVSEEKISRIEKSVNEAFGNKPPIAANVIDSKALAANE
                     IRVEKLALESQVGDLTLELNEKNAQLEEKDSVIQEKNNQIEERTSQLLEKQEEIDNYT
                     AQIKTYDVVLHVKPEKASQIHAQVINGEKCLVIPMRENENATINGVNTSL"
     gene            complement(4713..4922)
                     /locus_tag="DHV81_04335"
     CDS             complement(4713..4922)
                     /locus_tag="DHV81_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCI93722.1"
                     /translation="MRDEYDFSNAKRNPYAKKLKKQITINIDENTIDYFKVQAENSGI
                     PYQTLINLYLSDCVTQKRELQLSWK"
     gene            5126..7918
                     /locus_tag="DHV81_04340"
     CDS             5126..7918
                     /locus_tag="DHV81_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor G"
                     /protein_id="HCI93723.1"
                     /translation="MEQKMTNNHITIGILAHVDAGKTTLSEAMLYSTGQIRSLGRVDH
                     QDAYLDNDAQERERGITIFSKQARLDISRGTNAADTARTADVARTADTARTWHVVMLD
                     TPGHVDFSAEMERTLQVLDYAILVISATDGVQGHTRTLWRLLKHYNVPTFIFVNKMDM
                     QGTNAEKVQAELKSELSDGCVDFSSQDLFEELAMCSEPLLDEFMESGELTDAHIAQAV
                     AQREVFPCFYGSALKLDRVDTLIQGLSRFMCERNYPDEFSARVYKIGRDDRGSRLTFL
                     KVTGGCLRVRDKIEYKAHGGDEAKGHLIEKIDQIRKYSGEKYEIADVAEAGEIVAVTG
                     LSSTFPGQGLGSELQSNMPILEPVLNYRMILPDDVNPAAFMEKLKQLEEEDPQLYIVW
                     VEEFKEIHVQLMGQVQMEVLVRLIKDRFGVVVTFGPGSIVYKETIARAVEGVGHFEPL
                     RHYAEVHLLLSPAERGSGITVTSTCSEDVLDKNWQRLIATHVEEKEHRGVLTGSALTD
                     VKVTILTGRAHVKHTEGGDFRQATYRAIRQGLKSTESVLLEPYYSFILQVPMEYVGRA
                     MTDLEQRFARAESPQFATTAAREMATITGKAPVATMQDYVSLVHAYTKGLGHLTLELW
                     GYDECHNPAEVIAQMHYDSEEDFRNPTGSVFCAHGSGYVVPWDEVPEHMHLPYVYHGD
                     ESEEALAASARTQNAFSEEDAQALAGNRRRTSFEKAVSGMSSVELDAQLADVYAREFG
                     MGKNDIAEDQRRKWSGKKKNEYEGLSGKPRTVKHDKHGNPIYPKKSPGEEYLIVDGYN
                     IIFAWEDLKELSRINIDSARDALKDVLSDYQGYKGCHLLLVFDAYKVKGNAGKRETFH
                     NIEVVYTKQDETADAFIEKTVFGIRDRYKVTVATSDGLEQQTVMSLGALRMSARELKE
                     HIEMTKRNGMEAFISG"
     gene            7981..>8159
                     /locus_tag="DHV81_04345"
     CDS             7981..>8159
                     /locus_tag="DHV81_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCI93724.1"
                     /translation="MVVNESMYQLGSVRSAIRELFEYGKKRAAIVGKENVYDFSIGNP
                     SIPAPQIVNDTIKELV"
BASE COUNT         2332 a         1642 c         2078 g         1961 t
ORIGIN      
        1 cagcagctgc agccgcagcc gctgccagag cgaacagctc atcaagcagt ccaagctatg
       61 atggtggtgg agccggtaag ggcggaagta ttgccagtga ctatgcagca ggcggtggaa
      121 agaacccggg tgcttcaaca ggagtttcag gannnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagca
      301 caccgagata ttcacaggca tttaagtctg tgggacaggc cgtatccttc gataatatac
      361 agccgggaga cgtagtggta tatccgggac atgtagctat atatgcaggt ggtggtgtta
      421 tagtggaggc acagtccaca aaggcaggaa tcacagcaaa cagaagcgta cagtgtcata
      481 caatacttgc tatacgacgt cttgtatagg atggttagga gccgataagt atatttatta
      541 tacttaccgg ctcttttttn nccagccgca aggggctctc aatgcgctaa cgctatgctg
      601 tcaagaggct gtacgaatta tgcctgtatg gttttctatg ctcgacgaaa ccggctttta
      661 tgcgccgtcc atggcgcata acaccttgcc ctgccgtcca tggcaggaca tttctgggtt
      721 ccatgtgcat aattctacag actctaaaca gctccgctag tcgcttattg agagcccctt
      781 gcggctggtg gcatgtccgc gtcttacgcc acgacccaat gttagtaacc gtcaaaaact
      841 ccttgcaata aacgatagca attttcgcat ataaggtgta ttatagaagc agacggcacc
      901 aattcgcaca aaacgaacta gctagcctgt aaataaagta agaaaagcag gaggaacaag
      961 ccatgcagat ggaacaatta caaaaagaca tgattgcagc aatgaaagca agagacaagg
     1021 taagaaagga tgctatatcc tcacttgtat cggcagccaa aaaggcggca atagatgagg
     1081 gctgcagaga caatataccg gaggatcttg tcaacaaggt catccttaag gaaatgaaat
     1141 ccgtgaagga gcagattgac acatgccctg ccacaagaac agatctgctt gatgagtaca
     1201 ataccagata tgcaatcttt gaggagtacg ctcctgcaat gatgtcggca gaagaggtag
     1261 aaaaggagct cacagagcag ttttcggatg taatcgcaac aggaaacaaa ggacagataa
     1321 tgaaggcagt catgccgcat ttcaagggca aggctgacgg aaaggtcata aatcaggtgg
     1381 ttgcaaagct ttgcgcaaaa taagaaaaca catcacaaca attatacttg cagtgctggc
     1441 tgtcatagcc ggcgtgtatg catataatta tcatgatatg aagcagaata ttgtatacaa
     1501 tgagcatctg gaagacgtgg ctgttaacgt gaatgggaaa gagctgacat tgcgcgatat
     1561 ggcattttat gtggcatatg aggagatgaa tgtcgagaag caggctcttg tgtatgattc
     1621 cgataatcca aacaagtatt ggaatatcca cacaaacggt gaatttgtcc gtgttgcagc
     1681 gcgcaaagca gctatgtcca tggccattca cgatgagatt ttttatgaga tggccaaaaa
     1741 agagagcatt acacttactg acgacgagaa agcagctttg aaaaattccg agaaggactt
     1801 ctggtatgat ttgtctgata tagatggtgc aaagaagctt ggagtcgaaa aaaaggatat
     1861 ttattcgtcc atggaaaagt ctgcaatagc ccggaaatat caggaaatat atgcagggct
     1921 tgacaatgca gatataacgg attatgattt ctcgggaggc agatataaga agctgctgga
     1981 gaaaaataac tataaaataa aagaaaaagt gtggaaaaga gtcgatatgg gaaacgttac
     2041 tcttgatcac tagaaagaag attttatgtc aacaaaaatt ggaagaaatg acccttgctg
     2101 gtgtggcagc ggaaagaaat ataagacctg tcatgcggca tttgatatga agcttgaaaa
     2161 gcttgctgag gcacatcata agataccgag ccattcaatc atcaagacac ctgaccagat
     2221 agcgggaatc aaggagagcg ccaagataaa tgtcgcagtg ctcgactaca taggagaaca
     2281 tatccacgag ggtatgaaca cagcagagat tgataaaata gtctatgata tgacaaccag
     2341 catgggcggc atccctgcac cgcttaatta tgagggttat ccttacagcg tatgtacatc
     2401 ggtaaatgag caggtttgtc acggatttcc ttcaaaggac gtcatcttaa aggatggtga
     2461 catcataaat gtagactgtt ctacaatttt aaacggatat ttttcggatt catcaagaat
     2521 gtactgtatc ggaaatgtaa gccctgagaa gaaaaagctc gttgaagtca caaaggagtg
     2581 tgtggagctt ggactcaaag aggtaaagcc gtggggcttc ctcggagata tgggacaggc
     2641 ggtgcacgac catgcatttg caaacggtta cacagtagtg cgtgaaatcg gtggacacgg
     2701 agtgggactt gaatttcacg aggatccatg ggtaggctac aacactaagc gcggcaccga
     2761 catggtcatg gcacccggca tgatatttac catcgagcca atggtcaaca tgggcaaggc
     2821 cgatatctac gtagatgaca agaacaattg ggaagtatac accgacgacg gacttccatc
     2881 agcccagtgg gaaatcatgg tgctcgtcac agaggatgga catgaggttc tgagctggta
     2941 aacaggtaat tattataagg atattatttc ataggtgata acattataaa gggcgtgtga
     3001 aaaacgaaag tattttcaca cgccctttta tataataaaa taagataagc aacttgatgg
     3061 ttaaagcctg tccgggcgca aaaacgccct ctcggaagcg ttagcttcgg gagagcattt
     3121 ctgcatccac ggacatggct tataaactca tagtagattg cttcgcataa gtaccctaaa
     3181 tttaccggag agtatgcggt gtgttccgaa gaaaaatata aactgccacg gcgttccata
     3241 aaatgtggat acgacaattc atagcaaact atcaataaac tatccgcaaa tgctgccgtg
     3301 gctgatttta tatttttctg aggaatatac tgcatgctag gccggtatcc acagcctaga
     3361 tatgcgaagc cctataaact tgtattcacc ccatttatgg tcgcattctc attctccctc
     3421 ataggaatga cgagacactt ctccccattg atgacctgcg catgaatctg tgaagccttc
     3481 tccggcttta catgaagcac cacatcatag gtcttaattt gggcagtgta attatcaatc
     3541 tcttcctgct tctcaagaag ctgtgaggtg cgctcctcaa tctgattatt tttctcctgg
     3601 ataacagaat ccttctcctc aagctgcgca tttttctcat tgagctcgag agttaaatcg
     3661 ccgacctggc tctcaagagc aagtttttcc acacggattt cattagctgc aagagccttt
     3721 gaatcaatga cattggctgc gataggcggc ttgttaccga aggcctcatt gacggatttt
     3781 tcaatgcgtg aaatcttttc ctcagagaca tggcagtcac taagcacctt gttcatagcc
     3841 tcgctgtcga gaatgaacgg atcgtcgtca tcatcgtggg tctcagcgta gtcatcagcc
     3901 atctcggaca gattctgctg gatatcaagc agtttgtcat ccgtgctctc gtcctcggca
     3961 cctagcacgt ttctgacgat tgaatgaaaa gccatcttct gctcagtagc tgtgcgctcc
     4021 acaccgcagc cgagaccgtt tgtcataaac tcagagtgcg gctccttggt atttttcgta
     4081 tagaaaagag tggagtgtat gtcagtgctc ctatcattga aagcagggaa taaaaagcct
     4141 gtctcaggtg caccgaccac ccagtccctt atgcgggctc cgatacgctg ctccttttcc
     4201 agatagccaa ggcccggctt tgaaagtgtg acagggcaga tggcgcagag catgtatttg
     4261 tacacctcct cggactcatc aacgttgatg ttgtcctttg tacgtgtcat gacatcgtag
     4321 ctgtcgagat acaaaagaat caagtagttc ccggcctcat cgtaggtgtc gatgaccaga
     4381 tcatagtatg tatcgagcag agtctcatca ttaagctcgc tgtcgcgaag cgccatgaga
     4441 atctgctggc ggcctccgac ttcttcctcc tcaagcggaa actgcagatt gagcaggttg
     4501 tttccgagag tgcctgaaag caccttgttt gaaatctcga gatatttgta gtattcatcc
     4561 tcctccagag tgagaaattt gccaccgaat ttgcagacct tttcatggtt gcagtccacg
     4621 tagcagccga ccagacgact gaaggttgcc tggtcttttt ttaatcttcg ttttagttcc
     4681 aatacttctt ttttgttcat attatcctcg cattatttcc atgaaagctg gagttctctt
     4741 ttttgtgtga cacaatctga tagatataaa ttaataagtg tctggtaagg aattcccgaa
     4801 ttttcagctt gcactttaaa ataatctatt gtattttcat caatattaat tgtaatttgt
     4861 ttttttagtt tttttgcgta aggattgcgt tttgcgttgg aaaagtcgta ttcatctctc
     4921 atgattgatc ctcctaaaaa tcaaaacact tctataatta tattctccat aattacatta
     4981 taagtacgaa ataattattt gtcaattaag aaaaaagatg acagaccaac cacaaaatgc
     5041 tataatttag gaaatcaacg gattatgata taaaggctga cacagagaca tccacaggtg
     5101 tctctgttct tggattgagg cataaatgga gcagaaaatg acaaacaatc atattacaat
     5161 aggcattctc gcacatgtag atgcgggaaa gacaaccctg tctgaggcca tgctttacag
     5221 caccgggcag attcgctcac tcgggcgcgt ggaccatcag gacgcgtatc tggacaacga
     5281 tgcgcaggag cgtgagcgtg gaataaccat tttttcaaag caggcaaggc ttgatatttc
     5341 gcgtggcaca aatgcggcag atacagcacg tacagcagat gtagcacgta cggcagatac
     5401 agctcgtaca tggcatgtag tcatgcttga tacaccgggg catgtggatt tttcggcaga
     5461 gatggagcgt acccttcagg tgctcgacta tgcaatactt gtaatcagcg caactgatgg
     5521 agtgcagggg cacacgcgca cactgtggag acttttaaag cactataacg tgccgacatt
     5581 tatttttgta aacaagatgg atatgcaggg cacgaatgcg gagaaagtgc aggcagagct
     5641 aaagagcgag ctgagtgatg gctgcgtgga tttttcttca caggatttat ttgaggagct
     5701 tgccatgtgc agcgagcctc ttttagatga atttatggaa tcgggagagc ttacagatgc
     5761 gcatattgcg caggcggtgg ctcaaaggga ggtgttcccc tgcttttatg gctcggcact
     5821 taagcttgac agagtggata cgctgattca ggggctttcg aggtttatgt gtgaaagaaa
     5881 ttaccctgac gaattttctg cccgcgtata caagataggc agggatgaca gaggcagcag
     5941 gctgacattt ttaaaggtta ccggaggctg tcttagggtc agggacaaaa tagagtataa
     6001 ggctcatggc ggtgacgagg cgaaaggtca tctgattgag aaaatcgacc agataagaaa
     6061 gtattccggt gaaaaatatg agatagctga tgtggcagag gcgggtgaga tagttgctgt
     6121 cacaggtctt agctcgacat ttccggggca ggggctgggt agtgagctgc agtccaatat
     6181 gccaatactt gagccggtac taaactatag gatgattctg ccggacgatg taaatccggc
     6241 ggcatttatg gaaaagctaa agcagcttga ggaggaagac ccacagcttt acatagtgtg
     6301 ggtggaggag tttaaggaaa tccatgtgca gctcatggga caggtgcaga tggaggtgct
     6361 tgtgcgtctc ataaaggaca ggtttggtgt tgttgtcact ttcggaccgg gcagcattgt
     6421 atataaggaa acgatagcga gggctgtcga gggagtagga cactttgagc cgttgcgcca
     6481 ctatgcagag gtgcatctgc ttttgtcgcc ggcggagcgt ggcagcggaa tcacagttac
     6541 atccacttgc agtgaggatg tgcttgacaa aaactggcag cgcctcatag ccacacacgt
     6601 tgaggaaaag gagcacaggg gtgtgctcac aggctcagcg cttaccgatg tgaaggtcac
     6661 tattcttaca ggaagagcac atgtaaagca cactgaggga ggcgatttcc gccaggctac
     6721 atacagagca atccgccagg gacttaaatc cacagagagc gtgctgctgg agccatacta
     6781 cagctttatc ctacaggtgc cgatggagta tgtcggccgt gcaatgacag atctggagca
     6841 gagatttgca agagccgaga gcccgcagtt tgccacaacc gctgcgaggg agatggcaac
     6901 aattacagga aaggcaccgg ttgcgacaat gcaggattat gtgagtctgg tgcatgcgta
     6961 tacaaagggg ctcgggcacc tgacactgga gctgtggggt tatgatgagt gtcacaatcc
     7021 tgcggaggtg attgcgcaga tgcactatga cagcgaggag gatttccgca atccgacagg
     7081 ctcggtattt tgtgcgcacg gctcaggcta cgtggtgccg tgggatgagg tcccggagca
     7141 tatgcacctg ccgtatgtct accacggaga tgagtctgag gaggcacttg cagcgagtgc
     7201 ccgcacgcag aatgcatttt cagaggagga tgcgcaggcg cttgccggaa accgccgcag
     7261 gacgagcttt gagaaagctg tttccggtat gtcatcagtt gagcttgatg cgcagcttgc
     7321 agatgtttat gccagggagt ttggcatggg aaaaaacgat atcgcagagg atcagaggcg
     7381 caaatggtca ggtaaaaaga aaaatgagta tgaaggtcta agtggtaaac cgcgcaccgt
     7441 aaagcatgat aagcacggca atccgattta tccgaagaaa tccccggggg aagagtacct
     7501 gatagttgac ggatataata taatatttgc atgggaggat ttaaaggagc tttcacgcat
     7561 aaacatcgat tcggcgagag atgcgctgaa ggatgtttta tcggattacc agggctataa
     7621 gggctgtcat ctgctgctgg tgtttgatgc gtacaaggtg aagggcaatg ccggaaagag
     7681 agagaccttc cacaacatag aggttgtgta tacaaagcag gacgagacag ccgatgcatt
     7741 tatcgaaaag actgtgtttg gcatacgtga caggtacaag gtaacagtgg caacctcgga
     7801 cggactggag cagcagacgg tcatgagcct gggtgcactg cgaatgtcag cccgcgagct
     7861 taaggagcat attgagatga cgaagaggaa cgggatggag gcattcatat caggataaac
     7921 cctcatatca tattataata gtaaacatgc aagatcatca gagacaagga gacaaggaaa
     7981 atggtagtaa acgaaagcat gtaccagctc ggaagcgtgc gctcagcaat cagggagctt
     8041 ttcgagtatg gtaaaaaaag agcagcaatt gtaggtaagg aaaatgtata tgatttctca
     8101 ataggaaacc cgagcatacc ggctccgcag atagtaaacg acacaataaa ggagcttgt
//
LOCUS       DPUF01000116            5566 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_18076, whole
            genome shotgun sequence.
ACCESSION   DPUF01000116 DPUF01000000
VERSION     DPUF01000116.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_18076"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="DHV81_04350"
     CDS             <1..396
                     /locus_tag="DHV81_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93725.1"
                     /translation="ALKGAIFAAKIYEKLGFKVVPDGTESRHDIIQAVEFNNRDAMIA
                     FCEGIQAAAPVDSYVTPEPWAMPGYDSDVIMAAGAFVQGSSIELSADGPVKPPYAVYF
                     QGGLTWYHAKLGILMSLQKLYERNLVKLD"
     gene            467..1156
                     /locus_tag="DHV81_04355"
     CDS             467..1156
                     /locus_tag="DHV81_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HCI93726.1"
                     /translation="MHNNKTIKNLPASERPYEKFLSLGASGLSDADLLAIIIKTGTRD
                     KTVIDIAQDILSGRHGNLLNLYEMSYEELLSIPGIGQIKAIQLKAIAELSLRIAKTKK
                     VQSIRMNNPQTIADYYMEQMRHLTNEVVICAYFDVKSRFLGDKFISKGSLSSSVVDIG
                     SIIRTALDKNASKLVLLHNHPSGDCTPSRNDIAVTDRLIEGSGIFSIELCDHIIIGDN
                     EYYSFYENKLI"
     gene            1180..2184
                     /locus_tag="DHV81_04360"
     CDS             1180..2184
                     /locus_tag="DHV81_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HCI93727.1"
                     /translation="MSNNVYGIDLGTNNFKVYSKATGKTMLEKNTIAVIDKNQIYAYG
                     DSAYAMYEKAPETINVIFPVVEGVIADYNLLQTMIFDFLEHKTKARIKNAEFIIAVPT
                     DITDVEKRAFYELFYKSKSKPKKVMLCEKPIADAVGLGIDVNEPTGVMIVDMGADTTE
                     TSVISLGGLVLSDLLHFGGNRLDESIISFVRKEFNLVIGQKTAKLLKETIGSALPGRE
                     DSMVIVGRDVVSGLPIEMEIGSKVVYEAMKSNLESICTSIKMILEKTPPELAKDIIHS
                     GIYITGGGSQLAGLDQLFEQITGIKVICSECPEESAVRGLVQIVSDSKYKRLPFSIKK
                     "
     gene            2199..3149
                     /gene="mreC"
                     /locus_tag="DHV81_04365"
     CDS             2199..3149
                     /gene="mreC"
                     /locus_tag="DHV81_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCI93728.1"
                     /translation="MSKNFRNRKRKHNKKLPARYTLLIMTAVCFITMLLSLTLNISGG
                     PLKTAAGYVFIPMQKGINSFGIHISDKVTEIRTLKNVKAENKKLKAEVEELTTQLTTT
                     KLEQYELDNYRQLLDLDAKYPSYPKVAASVIAKDSGNWFSTFTIDKGKKDGVDVGMNV
                     LAGSGLVGIVTDAGDNFAKVRCIIDDASKVSGMVSTTEDNLTVSGNIKTMNEDKVITF
                     TELKDDDNKVKEGDPVVTSYVSDRYQQGILIGYVTKIENNSNNLTKSGTITPVVDFEH
                     IENVMVITQLKQTGDTKTADDTESTKTAGDSQTENSTENK"
     gene            3158..3679
                     /gene="mreD"
                     /locus_tag="DHV81_04370"
     CDS             3158..3679
                     /gene="mreD"
                     /locus_tag="DHV81_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCI93729.1"
                     /translation="MRRRIVLFIIITLCFLLQTTLFKTLSFANISPNLLIIAVSSFGF
                     MRGKKEGLWVGFFCGLLIDIFCGNYIGLYALIYMYIGFINGLFQKRFYPDDIKLPMLL
                     IGGSDLFYNLVVYFFMFLLRGRFSFLYYLKSIIIPEFVYTMVISILLYLVFLKLNQKL
                     EAHEKRRAKKFDL"
     gene            3669..>5566
                     /locus_tag="DHV81_04375"
     CDS             3669..>5566
                     /locus_tag="DHV81_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase"
                     /protein_id="HCI93730.1"
                     /translation="MIYDIKDFLKKFFSSRLFVLAAVIIFMFALLAERVFTLQIIKGA
                     DYQKNFIMLIKKPLSIEASRGNIYDVNGELLAYNQLAYSVVISDNGSYSSTKEHNRLL
                     NKELNEIINVIKNNGGSIYNDFPVVLNDDGTYSFTFTSETSKKRFLSDVFGKKYDKLE
                     YNKALGFDEANASAQNIIDFLSSDQNECFDISSKYDTQATYDIVVMRYAIKQNRFTKY
                     KTTTIAKDVNDSIVAYVNEHSDTLTGISVEEDTIRKYNYPEYISSLIGYTGKISTDEY
                     NELSKDDDSYTQNDMVGKSGLEQYYESYLRGKNGEKQVYVNNVGKINEVISQENPVSG
                     NDVYLSIDIKLQEATYKLLEQEIAGIVYSKIKSGEIPISDVYFALINNNVVDLTHFNA
                     SDASATEQAIYNSFSGQLQDALSTIDSELEGGTAGTASMSEQTLDYFTCVMSVLNDDG
                     LLLSKQIDTSDSTYASWKAGTLSPRDYLKYCISKQWIDITKLDVNQKYADSSEIYTAL
                     CSYIRDAMTDNKDFAKIIYKYMVNSGAVTGQQLCLLLFDQGVLDYDDATVNNIANGSI
                     SPYAFLMDKINNIEITPAQLALDPCTGSCVITDAKTGQLKALVSYPGYDNNKLANTVD
                     ADYYQSLR"
BASE COUNT         1798 a         1089 c         1205 g         1474 t
ORIGIN      
        1 gcattaaagg gagcaatctt tgctgcaaaa atctatgaaa agcttggttt taaggtagtc
       61 cctgacggta ctgaaagccg ccacgatatc atacaggctg tagagtttaa taaccgcgat
      121 gctatgatag ccttctgcga gggcattcag gcagcagcac ctgtagacag ctatgtgacc
      181 cctgagccat gggctatgcc gggctatgac agtgatgtca tcatggcagc aggcgcattt
      241 gtgcagggct catccattga gctttctgcc gatggccctg tgaagccacc atatgcggtt
      301 tatttccagg gaggactcac atggtaccat gcaaagcttg gaattttgat gtctttacaa
      361 aaactttatg agagaaatct tgttaagctg gattaggtgt gctacaatta actgaaaact
      421 ttgcttaaat ccgttgtcaa tcgcctggga gaagcgaaag ggattaatgc ataataacaa
      481 aacaattaaa aatctgccgg cttcagagcg accatatgag aaatttttat cgcttggagc
      541 atcaggcctg tcagacgctg atttacttgc catcattata aagaccggca caagggacaa
      601 gactgtgatt gatattgcgc aggacatact ttccggcagg catggcaatc tgctcaatct
      661 ctatgagatg tcctatgagg agcttcttag tatccccggg atagggcaga taaaagctat
      721 acagcttaag gcgatagcag agctgtcgct tcgcatagca aagaccaaaa aggttcaaag
      781 catcagaatg aataatcctc agacaatagc tgactactat atggagcaga tgaggcatct
      841 tacaaatgaa gtcgtcattt gtgcctattt tgatgtaaaa agcagattct taggagacaa
      901 gttcatatcc aagggttcac taagctcatc tgtagtggac ataggctcca tcatcagaac
      961 cgccctggac aaaaatgcat caaagcttgt tttattgcac aatcatccaa gcggagattg
     1021 tactcccagc agaaatgata ttgctgtcac agacagatta attgagggct cagggatttt
     1081 ttccatagag ctttgtgacc atatcataat cggagataac gaatattata gcttctatga
     1141 aaataaactt atataaacag aagacgaggt agaattatta tgagcaacaa cgtttacgga
     1201 attgatctgg gaacaaacaa tttcaaggta tactcaaagg ctacaggcaa gacaatgctt
     1261 gagaaaaaca caatagccgt aatagacaaa aatcagatat atgcatatgg agacagcgca
     1321 tatgccatgt atgaaaaggc gcctgagaca atcaacgtca tatttcctgt tgtagaggga
     1381 gttattgctg actataatct gcttcagact atgatttttg actttttgga gcacaagaca
     1441 aaggcacgta tcaaaaatgc cgagttcatc atcgctgtgc cgacagatat cacagatgta
     1501 gagaaaagag cattctacga gctcttctat aagagcaaat caaagcctaa aaaggtcatg
     1561 ctctgcgaga agcctatcgc tgatgctgta ggtcttggaa tagatgtaaa cgagccaacc
     1621 ggtgtcatga tagtagatat gggcgctgac acaacagaga catccgttat ttcactcggt
     1681 ggcctggtgc ttagtgacct gcttcatttc ggtggcaaca gacttgacga gtcaattatc
     1741 tcctttgtgc gaaaggagtt caatcttgta atcggacaaa agactgcaaa gctgttgaag
     1801 gagactatag gctctgcact tccgggaaga gaggacagta tggtcatagt gggacgtgat
     1861 gtggtaagcg gcctgcctat tgagatggaa attggttcaa aggtcgttta cgaggctatg
     1921 aagtcaaacc ttgagtctat atgcacatca ataaaaatga ttcttgagaa gactccgcct
     1981 gagcttgcaa aggatatcat ccattcaggc atttatatca caggaggcgg ctcacagctt
     2041 gcaggactcg accagctctt tgaacagatt acaggtataa aggtcatctg ctcagaatgt
     2101 cctgaggaga gcgcagtacg cgggctcgta caaatagttt ctgattcaaa atataagaga
     2161 cttccgttca gcatcaaaaa ataataaccg aggttaacat gagtaagaat tttcgcaatc
     2221 gaaagagaaa gcacaataaa aagctgcctg caagatacac actgttgatt atgacagcag
     2281 tctgctttat taccatgctg ttaagcctta ccttaaatat ctccggtggt cctttaaaga
     2341 ctgctgccgg atatgtattc atcccaatgc agaagggtat aaacagcttt ggaatccaca
     2401 tttcagataa ggttacagaa atacgcacat taaaaaatgt caaggctgag aataaaaagc
     2461 tcaaggccga ggtggaagag cttacaacac agctcacaac aaccaaactt gaacagtatg
     2521 agcttgacaa ttacagacag ctcctggatt tggatgccaa atatccatca tatcccaagg
     2581 tcgctgccag tgtcatagca aaggacagcg gaaactggtt ttctaccttt actatcgaca
     2641 aaggaaaaaa ggatggagtg gacgtaggca tgaatgtgct tgcgggaagc ggacttgtag
     2701 gtattgttac cgatgctggt gataattttg ccaaggtacg ctgtatcata gatgatgcca
     2761 gcaaggtcag tggcatggtt tccaccacgg aggacaacct caccgtcagt ggcaatataa
     2821 aaaccatgaa cgaggacaag gtaattacct ttacagagtt aaaggatgac gacaacaagg
     2881 tgaaagaggg cgatcctgtt gttacctcat acgtgtcaga ccgttaccag cagggcatac
     2941 tgattggcta tgtgacaaaa atcgaaaata attccaacaa tctgacaaaa tccggcacta
     3001 tcacaccggt tgtagatttt gagcatatcg aaaatgtcat ggtcataaca cagcttaagc
     3061 agaccggcga cactaagaca gctgacgaca ccgaaagtac aaaaacagcc ggtgacagcc
     3121 agactgaaaa ttcaactgaa aataagtaag gagcagcatg agacgtagaa ttgttttatt
     3181 tataataata actttatgct ttttattgca gaccacgctg tttaagactc tttcctttgc
     3241 caatatctca ccaaacctgt tgattatagc cgtatcatcc tttggcttta tgcgtggcaa
     3301 aaaggaagga ctgtgggtag gcttcttttg cggcctgctt attgacatat tttgcggaaa
     3361 ttatatagga ctgtatgctt taatatatat gtatatcggt tttatcaatg gattattcca
     3421 aaaaagattc tatcctgacg atataaagct tcctatgctt ctgataggag gcagcgattt
     3481 gttttacaat ctggtagtat actttttcat gtttttactg cgtggcagat ttagcttttt
     3541 gtactatctg aagtcaatta tcatacctga atttgtatac acaatggtta tcagtatact
     3601 tttgtatctg gtatttctta agttaaacca gaagctggaa gcacacgaga aaaggagagc
     3661 aaagaaattt gatttatgat ataaaagatt ttctgaagaa atttttcagc tcaagacttt
     3721 ttgtattagc agcagtaatt attttcatgt ttgccctgct tgccgaaaga gtctttaccc
     3781 ttcagattat taaaggtgca gattatcaga aaaacttcat aatgctgatt aaaaagccgc
     3841 tgtccataga ggcatcaaga ggcaatatat acgatgtaaa tggggagctg cttgcttaca
     3901 atcagcttgc atactctgta gtcatctcag acaacggcag ctattcatct acgaaggagc
     3961 acaatcgtct tttaaacaaa gagcttaatg aaattatcaa tgtgataaaa aacaacggag
     4021 gctccatata caatgatttt ccggttgttt taaacgatga tggtacatac agctttacat
     4081 tcacatctga aacctcaaaa aagagatttc tgtcagatgt gttcggaaag aaatatgata
     4141 agctcgaata caataaagca cttggctttg acgaggcaaa tgccagcgcc cagaatatta
     4201 ttgattttct aagctctgac cagaatgaat gctttgatat aagcagcaag tatgatacac
     4261 aggccactta tgatattgta gtgatgagat atgccataaa acagaaccga ttcactaaat
     4321 acaaaaccac tactattgca aaggatgtaa atgattcgat agtagcatat gtcaatgagc
     4381 attcagatac tcttaccggc atatcagttg aagaggatac catccgcaag tacaactatc
     4441 ctgaatatat ttcatcgctc ataggataca caggcaagat atctaccgac gagtataatg
     4501 agctatcaaa ggatgatgac tcatatacac aaaatgatat ggtaggtaag tcaggcttag
     4561 agcagtacta tgagagctat ctgcgaggca aaaacggtga aaaacaggtc tatgtaaaca
     4621 atgtcggtaa gataaacgag gtaataagtc aggagaatcc tgtttccgga aatgatgtct
     4681 acttaagtat tgacatcaag ctgcaggagg ccacatacaa gctgttagag caggagatag
     4741 ccggaattgt ttactccaaa ataaaatccg gtgaaatacc tataagtgat gtttactttg
     4801 cactgatcaa caacaatgta gttgacctga cacatttcaa tgcatcagat gcatcagcaa
     4861 ctgagcaggc tatatacaat tccttcagcg gtcagctgca ggatgcacta tcaactatag
     4921 acagtgagct tgaaggtggt acagcaggca ctgcttccat gtcggagcag acacttgatt
     4981 attttacatg tgttatgtca gtgctaaatg atgacggact gcttctgagt aaacagatag
     5041 atacctctga ttccacatat gcctcatgga aggccggcac gctctcgccg cgtgattatc
     5101 tcaaatactg catctcaaag cagtggatag acatcacaaa gcttgatgta aatcagaaat
     5161 atgctgattc aagtgaaatt tacacagcac tgtgttcata tatcagggat gccatgactg
     5221 acaataaaga ctttgccaaa atcatttaca aatacatggt aaattcaggg gcagtcacag
     5281 gacagcagct gtgccttctt ctttttgacc agggtgtact cgattatgac gatgcaactg
     5341 taaataatat cgccaacggc tccatcagtc catatgcatt tcttatggac aaaataaaca
     5401 atatagagat aacacctgca cagctggctc ttgacccgtg tacaggctcc tgtgtcataa
     5461 ctgatgcaaa aacaggacag ctaaaggctt tagtcagcta tccgggctat gacaacaaca
     5521 agctggcaaa tactgtagat gccgattact accagtctct cagaga
//
LOCUS       DPUF01000117            3528 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12341, whole
            genome shotgun sequence.
ACCESSION   DPUF01000117 DPUF01000000
VERSION     DPUF01000117.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12341"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(367..>1488)
                     /locus_tag="DHV81_04380"
     CDS             complement(367..>1488)
                     /locus_tag="DHV81_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCI93731.1"
                     /translation="LGGGIVRGSLVLVGGDPGIGKSTLLLQVCRELSQKKVPLLYVSG
                     EESLHQIKMRADRLGDFTDSLELLCETSLDIAKDVIQRKKPEVVVIDSIQTMYNEEIA
                     SAPGSVSQVRETTGVLMQLAKTLGISVFIVGHVTKDGAVAGPRTLEHMVDTVLYFEGD
                     RYESYRILRGVKNRFGSTNEIGVFEMKAEGLVEVENPSEYMLSGKPEGASGSIVTCSI
                     EGSRPILLEIQALVCHSFFNNPRRTANGTDYNKVNLLMAVLEKRANLSLSDCDAYVNI
                     AGGIRMNEPAIDLGIVLAIASSKKDIIVREDTICFGEVGLSGEVRGVTMSEQRVAEAK
                     KLGFKKCIVPKVCLAGVSHISGIEIVGVANIKEAIEAVR"
     assembly_gap    1489..1625
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1817..2230)
                     /locus_tag="DHV81_04385"
     CDS             complement(1817..2230)
                     /locus_tag="DHV81_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HCI93732.1"
                     /translation="MAVISELIRTEENGKLSFGDYTLAAKAKLDNFEKDGDIYKVKTF
                     KEITKLERNGLFVYESVPGTAVMDFEETADTVTFTVEGPEDAQITLGLEEETEYEIDI
                     AGAVAGTMKTNLGGKLSLSVELEGVDSVDIKVSRK"
     gene            complement(2278..>3528)
                     /locus_tag="DHV81_04390"
     CDS             complement(2278..>3528)
                     /locus_tag="DHV81_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpC"
                     /protein_id="HCI93733.1"
                     /translation="DLMDEAAARIRLKNLTSSDELLALKKEITDKQDRVEDALSKGDI
                     AAAGALMSEKEVLENKQQKLIRKNHRTGAKNQPVVGENEIADVVSGWTKIPVNKLTES
                     EAGRLAKLEQILHKRVIGQQEAVSAVAKAVRRGRVGLKDPKKPIGSFLFLGPTGVGKT
                     EVSKALAEAVFGKEDAMIRVDMSEYMEKHSVSKMIGSPPGYVGHEEGGQLSEKVRRNP
                     FSVILFDEIEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTIIIMTSNAGASSIIEP
                     KRLGFGAGDDEKQDHERMKNSVMEEVRRIFKPEFLNRIDETIVFRALNKDDMKHIVTL
                     LVKELKKRCKEQLDIELTVRDSAKSFIVDKAYDRKYGARPLKRKLQEEIEDRMSEDII
                     TGKIKRGNKVIISTKNKQISLTVE"
BASE COUNT          855 a          897 c          556 g         1083 t
ORIGIN      
        1 cagcttgatt attttatcaa atcgctgttc gtttgtcaag cactttttta tttttttgaa
       61 ctctgtttta agttctctgt cttgcgacag cttgtttatt ctatcaaaat gtttttcatt
      121 tgtcaagaac ttttttcata acttatttaa tcagcttatt tcaaagcaat atttataaag
      181 tcatgtctgt aaattgtaac tcacaagctc tacaactcaa tgtgtcccgc gagtcagctt
      241 tgatataata tcatcccggc acacatatgt caacggattt actgcaatta attaaagtag
      301 taaaactatc ttaaaaaaca caaattttct aaattgtctg tttattatat acaattctca
      361 caattactat ctgacagcct ctattgcttc cttgatattt gccacaccga caatctcaat
      421 tccactaata tgtgacactc ccgcaagaca cactttggga acaatacact ttttaaagcc
      481 aagcttcttt gcctcagcaa cacgctgctc ggacatggta actccgcgca cctctccgct
      541 taagcccacc tctccaaagc atatggtatc ctcacgcact ataatatcct ttttgcttga
      601 tgcgattgca agaactatcc caaggtctat cgccggttcg ttcatcctta taccgcctgc
      661 tatattcaca taagcgtcac agtctgacag cgacaggttg gctctttttt caaggaccgc
      721 catgagcagg tttactttat tataatctgt accatttgct gtacgccttg gattattaaa
      781 aaagctgtga cagacaagag cctgtatctc gagaagtatc ggtcggcttc cctctatgga
      841 gcatgtgaca attgaccctg atgctccctc tggcttaccg ctaagcatat actccgacgg
      901 attttctacc tcaaccagtc cctccgcctt catttcaaat acacctatct cattggtcga
      961 accaaatctg ttcttaacgc ctctaagtat cctgtacgac tcatatctgt caccctcaaa
     1021 ataaagcaca gtgtcaacca tatgctccag tgtccttggt cctgcaaccg caccatcctt
     1081 tgtcacatgt cctactataa aaacagaaat acccagagtc tttgcaagct gcataagcac
     1141 tccggttgtc tccctgacct gtgagacact gcccggtgcc gacgctatct cctcattgta
     1201 catagtctgg atggaatcta ttacaacaac ctccggtttt tttcgttgaa tcacgtcctt
     1261 tgctatatca agacttgtct cacataaaag ctccagcgaa tccgtaaaat cgccaagtcg
     1321 gtcggcacgc atctttatct gatgcaggga ttcctcacct gacacataaa gtaatggcac
     1381 ctttttttgt gaaagttccc tgcatacctg aagtagaagc gtggattttc ctatacccgg
     1441 atctcctccc acaagcacaa gtgaccccct cacaattcct ccaccaagnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnncttat cgacttttta tctacggttt cctctacaaa tgtattccat tccttacagg
     1681 ccgggcactg tcccatccat tttgccgatt cataaccaca ttcctggcag aaaaatacag
     1741 aggtctttcc ttttgccata ttgttctcct ttataaaata caaaaagccc ggctttcacc
     1801 gggctaaaaa tatgctctat tttcttgata ctttgatatc gactgaatca acaccctcaa
     1861 gctctacgct taagctgagc ttgccgccga gatttgtctt catagtacct gcaacagctc
     1921 cagctatatc aatttcgtac tctgtctcct cttcaaggcc aagagtaatc tgagcatcct
     1981 caggtccctc cacagtaaat gtcactgtat cagcagtctc ctcgaaatcc ataaccgctg
     2041 ttcccggaac agactcgtat acaaagagtc cgtttctttc gagctttgta atctccttaa
     2101 aggtctttac cttgtagata tctccatcct tctcaaaatt gtcaagcttt gcctttgcag
     2161 ccaatgtata atctccgaag ctgagcttgc cgttttcctc agtacggatt aattcactta
     2221 ttacagccat ttttatcctc ccgtagctat attgatatat taattataaa tccaattcta
     2281 ctcaacagtc aatgaaattt gtttgttttt cgtagaaatt attaccttat tgccgcgctt
     2341 aattttgccg gtaataatat cctctgacat acggtcttca atctcctcct gaagttttcg
     2401 tttaagaggt cttgcgccat attttctgtc ataggctttg tccacgataa aggattttgc
     2461 actgtcgcgc actgtgagct ctatatcaag ctgttcttta cagcgtttct taagttcctt
     2521 tacaagaagt gtcactatat gcttcatgtc atccttgttg agagctctaa atacaatagt
     2581 ctcgtcgatt cggttcaaaa actcaggttt aaatatacgg cggacctcct ccatgacact
     2641 gttcttcata cgctcgtgat cctgcttttc atcatcacca gcgccaaagc caaggcgctt
     2701 tggttctata atagaagaag ctcctgcatt tgatgtcatg atgattattg tattcttgaa
     2761 atcaaccgtg cgcccctgtg aatcagtgat tctaccatca tcaagcacct gtaaaagtat
     2821 attgaacaca tcaggatgtg ctttttctat ctcatcaaac agtataacag aaaaaggatt
     2881 gcgtctgacc ttctctgaga gctgtccgcc ctcctcatgg cccacatatc ccggtggtga
     2941 accaatcatc tttgaaacac tatgcttttc catgtactct gacatgtcaa cacgtatcat
     3001 ggcatcttcc ttgccaaaga cagcctcagc aagcgccttt gacacctccg tcttacctac
     3061 tcctgtcggt cccaaaaaca ggaatgagcc aatcggcttc ttaggatcct ttaaaccaac
     3121 gcggcctcgt ctgactgcct ttgcgacagc agaaacagcc tcctgttgtc ctattacacg
     3181 cttgtgaagt atctgttcaa gctttgcaag tcgtcctgcc tctgactctg taagcttatt
     3241 tacaggtatt ttggtccatc ccgatactac atccgctatc tcattttcgc cgactacagg
     3301 ctggtttttg gctcctgtgc ggtgattctt cctgataagc ttctgctgtt tattttcaag
     3361 cacttccttt tctgacataa gcgcgccggc agctgcgata tctcccttcg acaaagcatc
     3421 ttccacccgg tcctgcttat ctgttatctc ttttttcaga gcaagcagct cgtcagatga
     3481 tgtcaaattc ttaagcctta tcctggctgc cgcttcatcc atcaggtc
//
LOCUS       DPUF01000118           11603 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13591, whole
            genome shotgun sequence.
ACCESSION   DPUF01000118 DPUF01000000
VERSION     DPUF01000118.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11603)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11603
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13591"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="DHV81_04395"
     CDS             <1..1069
                     /locus_tag="DHV81_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCI93734.1"
                     /translation="VAVGISKPLDELKQRLRTFAQGDLEAPFPAVDSQDEIADMVGVA
                     KNMAADLKTIISDSDKLLGKMAEGDYTVSSDMEDKYTGDFIGLLMAMRQMKTQMNDVM
                     SHINEISSLVTAGSNNLAQAAQEIAEGTMDQSAAIEELQATFADITGGVEKTSEKLND
                     TYRIAQEYAEEADHSHTEMQGMVDVIGRINDTSKQIENIISEIEDIASQTNLLSLNAA
                     IEAARAGEAGKGFAVVAGQIRSLSEQSAKAAVDTRQLIESAIAVSNEGNEAAERVSTS
                     IEKVINGMKEVADSSQKLSEIAEEQAKAMEQAEAGINQISDVVQSNSANAEETSATSE
                     ELSAQAETMNELISKFILEKK"
     gene            1336..2217
                     /locus_tag="DHV81_04400"
     CDS             1336..2217
                     /locus_tag="DHV81_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCI93735.1"
                     /translation="MGSNNDISLKALAKINLGLDVVRRREDGYHEVRMIMQTIHLYDR
                     LDIKRTKEPGIQIQTNLSFLPVNENNLIYKAAKLLMDEFSITDGVSVKLDKRIPVAAG
                     MAGGSTDAAAMLIGVNRLFSLGLTKRQLMERGVQIGADVPYCIMRGTALAEGIGEELS
                     PLPPMVKCPVLIAKPSISVSTKFVYQNLKLDDTTIHPDIDRLIDDIKAKNLHDIAAHM
                     GNVLETVTIPNYPVIDEIKKHMLSNGAVGAMMSGSGPTVFGLFDDEDTAKKAYKAMRS
                     SHLARQVYLTSVYNNRK"
     gene            2305..2991
                     /locus_tag="DHV81_04405"
     CDS             2305..2991
                     /locus_tag="DHV81_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI93736.1"
                     /translation="MTDNLTLNMDAYLPLRDVVFNTLREAILKGELKPGERLMELQLA
                     SKLGVSRTPIREAIRMLEQEGLAVTMPRKGAEVAKMTLKDMEDVLEVREALDELAAKI
                     ACKKISDEQLANLKTIKDEFKRSMDSGDVKKIAEEDVKFHDAIYEATNNAKLVSMMNN
                     IREQMYRYRVEYLKDPKNYPMLVKEHDAIYRALEARNMELVTTEMHTHVANQAVAVKA
                     VIQKQDEEKK"
     gene            3217..>3473
                     /locus_tag="DHV81_04410"
     CDS             3217..>3473
                     /locus_tag="DHV81_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HCI93737.1"
                     /translation="MTTNIDENIKSFRQIYSDCSDIKMQEMYLGRDASIKCFVAYIEV
                     TCAGSGINNSAFGRFTSYLEGIDRDQVKEVLDKNQAALSEFA"
     assembly_gap    3474..3666
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3667..4710
                     /locus_tag="DHV81_04415"
     CDS             <3667..4710
                     /locus_tag="DHV81_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HCI93738.1"
                     /translation="IKINTALIRRRLRSTRLKCKEVKKGLRGHSNVDILYVRDLVKPG
                     LVEEVEKNLDSYVIDHVGDSGVLEQFAEAKWYSPFPQLQTTKRPDVAVNALLEGRVVV
                     LCDNSPIAIILPTTMNNFLKTADDYYNRTIAASFARLIRYVAAFMSFTLPGLYLAVTN
                     FHTQILPTPLILAFYEARLGCPFPQLIEVLMMELSFELLREAGIRLPGAMGNTIGIVG
                     GLIIGQAAVDANLVSPIVVILVAFTALCSFAIPSEEFAFSFRILKFAVIMMSAWLGYF
                     GFLISLMVILLHLAKLKSCGYPYMMPFVGSELTGGEDEKDSIIRFPLRRLWRRPVFAR
                     EKECRKLKGNKDD"
     gene            4703..6439
                     /locus_tag="DHV81_04420"
     CDS             4703..6439
                     /locus_tag="DHV81_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HCI93739.1"
                     /translation="MTKNMSTSYTFAQNESISPRQLYRLYVFNLLGVGTLVLPNNLAK
                     LGKYGFISIALGVFMAWLFMWIVSEVREQKKSIYDRSFGKTTHAKMLIYDLIIVIYEL
                     SQAAFLAWIFVKLIRDSLIPDESFTVVLLVIMAVCAYALSGGVECRARVYEVVFFFVL
                     IPLAAMLLFALSDVRLDYLMIKDRVGVDFGIADIFAGAYYVFAASISVFNILFVRERT
                     ASQIRWSVSKAILTYAGILFLLYAVLLGSFGKYSLSEIEFPAVVLMSDVQIKGSFFKR
                     ADALMLSVWFFTLFSVLIMSLYYAVLRCENFAENTREIIFTFNKNRHSCSNKQFGSDR
                     QKSHDKEKSTMRSWCIIFVIAVTVTLAYILESGDEIVKKYLGFLMCIAIPVIVVLTIG
                     LMLTGCSAVELEERCFPTLAAVDVVAVDTDEITDKEVTGKDVTDYKEYVSHRDDLANE
                     SSGYIEFYYNMDKSYEPEYADDIKTAVDSFEERLSQKADTNHLKVILIGKTLRNDKAA
                     YASFMEYCKTSKKFPRNTYVCIADDINDIFDNMGDYYEQKMNKENHEDGEPIITLGTL
                     LDDYTNETHGAW"
     gene            6491..7126
                     /gene="spoIIR"
                     /locus_tag="DHV81_04425"
     CDS             6491..7126
                     /gene="spoIIR"
                     /locus_tag="DHV81_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HCI93740.1"
                     /translation="MRKKIIITTIAIISLTAAIAAKNHTPVANTNSIACTADMQKSIA
                     GKILRFHVLANSDSEADQNVKKQVRDAVGAYIEPYLLECENIEETRATVNDHMDEIIA
                     VSKETLAANGFSYGASAELTHTDFPEKTYGDYTFPEGNYEALEITLGDGAGHNWWCVL
                     YPNMCFRGSVYEVVEDEAKENLKEVLTPDEYESIFDSGKYEIKLKILDIFR"
     gene            7234..7677
                     /locus_tag="DHV81_04430"
     CDS             7234..7677
                     /locus_tag="DHV81_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCI93741.1"
                     /translation="MTKDVVVSLKGLQLAPDQQSDAVEVIAPGEYYIRNNKHYVLFEE
                     IMEGETQTLKNMLKFNDNYLEFTRKGPMSVHMIFEKGKKNLTYYYTPFGSIQIGIDAT
                     SIEVKETEDEIRAEVKYALDVNYEFVGDCHINIAVKKKGAAVKIA"
     gene            complement(7808..8341)
                     /locus_tag="DHV81_04435"
     CDS             complement(7808..8341)
                     /locus_tag="DHV81_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93742.1"
                     /translation="MNPALIVLIVIAVVLVVAFIVLTILGKKAQKRKEEQDEQIAAAS
                     QTVNMLIIDKKRMKLKDAGLPQQIIEQTPKLMRRSKLPVVKAKVGPRVMSLIADEQIF
                     DEIPLKKEVKASVSGIYITAVRGIRGPLEKPKKKKGFRARMAENYSKANEQLKAEEAA
                     KAERKAAKEAAKKAKQK"
     gene            8494..>11603
                     /locus_tag="DHV81_04440"
     CDS             8494..>11603
                     /locus_tag="DHV81_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HCI93743.1"
                     /translation="MKKIALLADGWRRYVTYSWVEGIMEGSKELGLDVCLYFYNTNGT
                     WSQDSKFNKGEYALNDLPDLNSFDGVVFDCTNTTNLDEIQYMVRKLQSVNVPVVSIGY
                     KVDGFYYVGNDNKRLFRKVMDHMYYAHGCKSFVFAGGPPYHYENRMRFEAFKEALSDY
                     GIPLTADMYMFGDFDYQTGVRYMSDWHESKKPLPDVFLCANDNIAAGLCATAEVLGYK
                     VPQDFKVTGFDNLDKAAYFNPQITTVDNNRGNIGRNALEIFKALWNGTGDASDKYLDS
                     EFIPAESCGCPNTGRVDYRNYIKNIIKGSVAREQEEDAVMILQKELEECNEYYDLFER
                     YSDYIQSMKCDGVYVVGVSDLAAARNNAHFRKHGYDIDDEVVLYADDKDNGKLEFKSV
                     NDLMQYMQSVDKNTCYMYCSLHFRDEIVGYVILRNPEFLYDHPEQFDIQSALLKRLEN
                     LFKQKVLENTNNELKNLYNHDALTGLYNRVACNEMVIPMFAELEAQNVGCTIVFLDVD
                     DFKDINDTYGHQYGDELLKTVARVLDEQKPEGSMVYRFGGDEFIVFIPGDRHDTAEKY
                     IKRVYDIMEQHSLFISHGIIYTRPGSGKSFDDYLVSADTKMYQLKQQRKQKKDSNFLK
                     GVDISSVPMMVDNNIQIMDREGHVCRVFDFLKLNNVNSVRLRIWNEPDKVPESKGYCS
                     LTYVLEMAHIIKKYKMHFLLDFHYSDYWADPGQQNKPDAWKNLSFDELKEAVHKYTYD
                     VLYRLKIMDCAPDMVQIGNEIRSGMLFPDGAVPQYRQLAALVNEGIRAVRELLPETKV
                     MIHLDQGGRYNCIMPWFDSMFNAGMLPIDAIGLSFYSFWHGTFMDLKDTMSRLIKLYN
                     LPVYIVETAHPWRHCKGDHIPKELMETAGLDAGSAEQKKSLEIIMQIAAEVSKDTGKT
                     GVYYWEPVGVPGKGMGTWFENMGMFDEHGRALPGWDAIRDFDPKNPPIKELDKYIESL
                     YEYEETPEVEDFMKLLMIHGNLISNPEFKDGFNNWQIETSLEEGQYTLGKDGVFISSD
                     ANFDYS"
BASE COUNT         3529 a         1974 c         2873 g         3034 t
ORIGIN      
        1 agttgcagtt ggtatttcta aaccactcga tgagttaaaa cagagattac gtacatttgc
       61 acagggtgat cttgaggctc cattcccggc agttgattca caagatgaaa ttgctgatat
      121 ggtaggggtt gcgaaaaaca tggcagcaga ccttaagact atcatatctg actctgacaa
      181 gcttcttgga aagatggcag aaggagatta tacagtttca tcagacatgg aagataaata
      241 taccggagat tttattggac ttctcatggc aatgcgtcag atgaagacac agatgaatga
      301 tgttatgtca catatcaatg agatatcatc acttgttaca gcaggttcta acaaccttgc
      361 tcaggcagca caggaaatag cagaaggtac tatggatcag tcagcagcca ttgaggagct
      421 tcaggctaca tttgcagata tcacaggcgg tgttgagaaa acatctgaga agctcaatga
      481 tacatacagg attgcacagg agtatgctga ggaagcagat cacagccata cagagatgca
      541 gggcatggtt gatgttatcg gacgcatcaa tgatacatct aagcagattg agaatatcat
      601 ttctgagatt gaggatattg ccagtcagac aaaccttctt tcactcaatg cagctatcga
      661 ggcagcgcgt gcaggtgagg caggtaaagg attcgcagtt gttgcgggac aaatcagaag
      721 tctttcagag cagagtgcca aggctgcggt tgacacacgt cagcttatag agagtgccat
      781 tgcggtatct aacgagggta atgaagctgc agagcgcgta agtacttcta ttgaaaaggt
      841 tatcaatggt atgaaggaag ttgcagattc ttctcaaaaa ctcagcgaga ttgcagagga
      901 gcaggcaaag gctatggagc aggcagaggc cggtattaat cagatatcag atgttgtaca
      961 gtccaactca gcaaatgcag aggaaacatc agcaacaagt gaggagcttt cagcacaggc
     1021 ggagacaatg aacgagctga ttagcaaatt tatattggaa aagaaataga aaaattgtac
     1081 atacaagttt agagaaactt tttaaaaaaa gccccacaag cattattgat atgctgtgag
     1141 gctttttgtt gtggtgcgtt agagggttac tttttaaatc tagtaaacat gtactatgtg
     1201 aacatagttt tttgtgcgat acgtggaaaa tctgaaaagt aatgaaaatg aacaattaaa
     1261 tgagaatatt taaatttgct ctttttggca gggtgtgtta taatccttgt atacaaaaaa
     1321 cagaggtaat acaatatggg tagtaataac gatatttcat taaaagcatt agccaagatt
     1381 aatctggggc tcgatgttgt gaggcgcaga gaggacggat atcatgaggt ccgtatgatt
     1441 atgcagacca ttcatctgta tgacaggctt gatatcaaaa ggacaaagga gcctggcata
     1501 cagatacaga ccaatctgtc ttttctccct gtaaacgaga acaatctcat atacaaggct
     1561 gcaaagcttc tgatggatga gttttctata actgacggtg tatcagtgaa gctggacaag
     1621 cgtattcctg ttgcagcagg gatggcgggg ggcagtactg atgcggcagc tatgcttata
     1681 ggtgtaaaca ggcttttttc actgggactg acaaagagac agcttatgga aagaggtgtg
     1741 cagatagggg cggatgtacc atactgtatt atgagaggca cggcgctggc cgagggcata
     1801 ggagaggaac ttagtcctct tcctccaatg gtaaagtgtc cagtgcttat tgcaaagccg
     1861 tccatctcgg tatccacaaa atttgtgtac cagaatctga agcttgatga tacgactatt
     1921 catccggata tagaccgcct gatagatgat ataaaggcta agaatctgca tgatatcgca
     1981 gcgcatatgg gaaatgtctt agagactgtg actataccaa attatccggt gatagatgag
     2041 ataaagaagc atatgctttc aaatggcgcg gtaggagcca tgatgagcgg tagtggtccg
     2101 acggtttttg gtctgtttga cgatgaagat actgcaaaga aagcgtacaa agccatgaga
     2161 agctcacatc ttgccagaca ggtgtatctg acatctgtat acaataatcg caaataacgt
     2221 gatatccgga tagcacagcg ggtgctttat agttactttt ttcttatatg tgagaatgat
     2281 aatacaagag ataggagaag tttgatgact gataatttaa ctttaaatat ggatgcatat
     2341 ctgccactgc gtgatgtggt gtttaacaca cttcgtgagg caattttaaa gggtgagctc
     2401 aagccgggag agagacttat ggagttgcag cttgcgtcaa agcttggagt cagccgtaca
     2461 cctatacgtg aagcaatcag aatgcttgag caggaggggc ttgcagtcac aatgccgaga
     2521 aagggcgcag aggttgccaa gatgactctc aaggatatgg aggatgtgct tgaggtgaga
     2581 gaggctcttg atgagcttgc agccaaaata gcctgtaaga aaatcagtga tgagcagctt
     2641 gcgaatctaa aaaccataaa ggacgagttc aagaggagca tggactcagg agatgtgaaa
     2701 aagatagctg aggaggatgt gaagtttcac gatgctatct atgaggcaac aaacaatgcg
     2761 aagcttgtat ctatgatgaa caatatccgt gagcagatgt accgctacag agtggagtat
     2821 ttgaaagacc caaagaatta tccgatgctt gtgaaggagc atgatgccat ctacagggcg
     2881 cttgaggcaa gaaatatgga gcttgtgact acagaaatgc atacgcatgt ggccaatcag
     2941 gcggttgctg tgaaggctgt gatacaaaag caggatgaag agaagaaata aaacagaaat
     3001 ataacaagat tatagcagac aaagataata aaatacaaaa taatataaca aaaccagggg
     3061 ctgccggttg cactgacgtg tgaacggcgg ccttttttag tggcttagat aatagcaaaa
     3121 aataagtaat cagggatatt tgcgggcttg tatggtcata ctatatcagg gattttaaat
     3181 cttacgggat ttattgggta tataggagag ttaaagatga ccacaaacat agatgagaac
     3241 ataaaaagct tcaggcagat atattctgac tgctcagata tcaagatgca ggagatgtat
     3301 ctggggcgtg atgcttccat aaagtgtttt gttgcatata ttgaggtgac atgtgcaggc
     3361 tccggtatca ataacagtgc gtttggacga tttacatcat accttgaagg gatagaccgg
     3421 gatcaggtta aggaagtact tgacaaaaat caggcagctc tatccgaatt tgcnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnataa agataaatac tgcacttatc aggcgcaggt taaggagcac tcgcttaaag
     3721 tgtaaagagg tgaaaaaggg gcttcgcgga cacagcaatg tggacatatt gtatgtgaga
     3781 gacctcgtaa aaccggggct tgtagaggaa gtggaaaaga atctggacag ctatgtgata
     3841 gaccatgtgg gtgactcggg agttcttgaa cagtttgctg aggcaaaatg gtattcaccg
     3901 ttcccacagc ttcagaccac aaagcggccg gacgtggcgg taaatgcgct cctggagggg
     3961 cgggtggtgg tgctgtgcga taattcaccg atagcaataa tactgccaac caccatgaac
     4021 aattttttaa aaaccgcaga tgattactat aacagaacga tagcggcaag ctttgccaga
     4081 ttgataaggt acgtggcagc ctttatgtca tttacattgc cggggcttta tctggcggtc
     4141 acaaattttc atacacagat actgccgaca ccactcatac tggcgtttta tgaagcaaga
     4201 ctcggctgcc catttccaca gctcatagag gtgctcatga tggagctgtc ctttgaactg
     4261 cttcgcgaag ccggcataag acttcccgga gccatgggaa atacgatagg catagtcgga
     4321 ggcctcataa tcggacaggc agcagtggat gcaaatttag tgagccctat agtggtcatc
     4381 ctggttgcat tcaccgcact ctgctcgttt gccataccga gcgaggagtt tgcattttca
     4441 tttaggatac tcaaattcgc tgtaataatg atgtcggcat ggcttggata ttttggattt
     4501 ttaataagtc tgatggtgat acttttgcat ctcgcaaagc tcaaaagctg tggttaccca
     4561 tacatgatgc catttgtggg cagcgagctg acaggcggcg aggatgaaaa ggacagtatc
     4621 atacgatttc cgcttcgcag actatggagg cggcctgtat ttgcaaggga aaaggagtgc
     4681 agaaagctga aaggaaataa ggatgactaa aaatatgagc acaagctaca catttgcaca
     4741 aaacgaaagc atatcgcccc gccagctata cagactatac gtctttaatc tgcttggagt
     4801 cggcacactg gtgcttccaa acaatctggc gaaactggga aagtatggtt ttatctcaat
     4861 cgcacttggt gttttcatgg catggctatt tatgtggata gtaagcgagg tacgagaaca
     4921 gaagaaaagt atatatgaca gaagctttgg taaaacaaca catgcaaaaa tgttaattta
     4981 tgatttaatt attgtgatat acgaactgtc acaggccgca tttcttgcat ggatatttgt
     5041 aaagctgata cgtgacagcc ttataccgga tgagtcattt acagttgtac ttcttgtgat
     5101 aatggcagtg tgcgcttatg cgctaagtgg cggagtggag tgccgtgcca gggtatacga
     5161 ggtagtattt ttctttgtgc tgataccgct tgctgctatg cttttgtttg cgctatcgga
     5221 tgtgagatta gactatctga tgattaagga cagagtcggt gtggactttg gcatagcgga
     5281 tatatttgcg ggcgcatact atgtgtttgc agcatccatt tccgtgttca atatactgtt
     5341 tgtaagggag cgcactgcct cacagatacg atggagtgtg agtaaagcaa tactcacata
     5401 tgcaggcatt ctttttttat tgtatgcagt attgcttggg agcttcggaa aatattcatt
     5461 gtcagaaatc gagtttccgg ctgtcgtact gatgagtgat gtgcagataa agggaagctt
     5521 ctttaagagg gcagatgcac tgatgctgtc ggtgtggttt ttcacgctgt tttcagtgct
     5581 cattatgagc ctttattatg cggttttaag atgtgagaat tttgctgaaa atacgagaga
     5641 aattatattt acatttaata aaaaccggca ttcctgtagc aataagcaat ttggctctga
     5701 cagacagaaa agtcatgaca aggaaaagag cactatgaga agctggtgca tcatttttgt
     5761 catagcagtc acagtgacct tagcatatat tcttgaaagt ggagatgaaa tcgtgaaaaa
     5821 atatcttgga tttttaatgt gcatagcgat accggtcata gtagtgctga cgataggtct
     5881 catgcttacc ggctgtagtg cagtggagct tgaggagcgt tgctttccga cacttgctgc
     5941 ggtggatgtt gtagctgtag atactgacga aataaccgat aaagaagtaa caggtaaaga
     6001 tgtaactgat tacaaagaat atgttagcca cagagatgat ttggcaaatg aaagtagcgg
     6061 atatatagag ttttattata acatggacaa atcctatgag cccgaatatg cagatgatat
     6121 caaaacagcc gtggacagct tcgaggagcg gctgtcacaa aaagccgata ccaatcatct
     6181 gaaggtgata ttaataggaa aaacgcttag aaatgataaa gcagcttatg caagcttcat
     6241 ggaatactgc aagacatcca aaaaatttcc gagaaacaca tatgtgtgca tagcagatga
     6301 tataaacgat atctttgata acatgggtga ttattatgag cagaaaatga ataaggagaa
     6361 tcatgaggat ggggaaccga taattacact tggcacctta ttggatgact atacgaatga
     6421 aactcatgga gcatggtaaa aatttcaaaa aggatgaata atcttttcaa ataaggcaat
     6481 acttgtttac atgagaaaga aaataataat aacaaccata gcaataatat ctcttacggc
     6541 agcgatagct gcaaaaaatc atacacctgt cgcaaacacc aatagtatag cgtgtacagc
     6601 cgacatgcaa aaatccatcg ccggcaaaat cctgcgtttc cacgtgctgg caaacagcga
     6661 cagcgaagca gaccagaatg tgaaaaagca ggtgcgcgat gcagtagggg cttacataga
     6721 gccatatctg ctggagtgcg aaaatatcga agaaaccaga gccaccgtaa atgaccatat
     6781 ggacgaaata atagcggtat caaaggagac gcttgcagca aacggttttt catatggcgc
     6841 atcggcagag cttacacaca ccgatttccc ggaaaagacg tatggggact atacatttcc
     6901 tgagggaaat tacgaggcgc ttgaaataac actgggcgat ggagccggtc ataactggtg
     6961 gtgcgtgctg tatccaaaca tgtgctttag gggcagtgtg tacgaggtgg tggaggacga
     7021 agcaaaggaa aatcttaagg aggtactcac tccggacgag tatgagagca tatttgacag
     7081 tggaaaatat gaaataaaac taaaaatcct tgatattttc agataaaaca gttagtatat
     7141 tcatgctgaa aaaatatatt aaaagaaaat atgtgatgag aaattattaa agcgtgaatt
     7201 aatttagata tgcaagagga taaggagaaa gaaatgacaa aggatgtagt agtatcatta
     7261 aagggcctgc agctcgcacc ggaccagcag tcggatgcgg tagaggtgat tgccccggga
     7321 gagtattata taagaaataa caaacactat gtgctttttg aggagataat ggagggtgag
     7381 acccagacac tcaaaaatat gcttaaattc aatgacaatt atcttgagtt cacccgcaag
     7441 ggacctatga gtgtacatat gatatttgag aagggaaaga aaaacctgac atattattat
     7501 acaccgtttg gaagtatcca gataggtatt gatgccacaa gcatagaggt aaaggagact
     7561 gaggacgaga taagggcaga ggtaaagtat gcacttgatg tcaactatga atttgtaggc
     7621 gactgtcaca tcaatatagc tgtgaagaaa aaaggcgcag cagtcaaaat tgcataaaag
     7681 tctgccaagc ctatggggta aaaaatatat aaagcatata tggataaagc atataaaaaa
     7741 cagagcatgt gaggatgaaa gccatcccac atgctctgtt tatgtacatg aaaattaaat
     7801 catgatatta tttctgctta gcctttttgg ctgcctcttt agcagctttt ctttcagcct
     7861 ttgcagcctc ttcagccttg agctgttcgt tggccttgct gtagttttct gccattcttg
     7921 cacggaagcc ttttttcttc ttaggcttct caagaggtcc tctgataccg cggacagctg
     7981 tgatgtagat accgctgaca gaagccttaa cttctttctt taaaggaatc tcatcaaaaa
     8041 tctgctcgtc tgcgataaga gacatgactc ttggtccgac cttggccttg actacaggca
     8101 gctttgagcg gcgcataagc tttggtgtct gctcaataat ctgctgtggg agaccggcat
     8161 cctttagctt catacgcttt ttgtcaatga ttagcatatt gactgtctgt gatgcagcag
     8221 ctatctgttc gtcctgctct tcctttcttt tttgtgcttt tttaccgagg attgttaata
     8281 ctataaatgc taccacaagc actactgcga taacaataag tacgattaat gctggattca
     8341 aaacgtatcc ctcctaaaaa tttgcttatt taaactaatt ctatcaaata tgctaaaaaa
     8401 aatcaatctt tattgcaaga ataggtaaaa tcgtagtata atagaactat attttggaaa
     8461 aggtctttag aagacgcaaa ggggtattta ttaatgaaga aaattgctct tctggctgat
     8521 ggctggagaa gatacgtaac atattcgtgg gttgagggta tcatggaagg ctctaaagag
     8581 ctgggactgg atgtgtgcct gtatttttac aacaccaacg gaacctggag tcaggattca
     8641 aagtttaaca aaggcgagta tgcgctgaac gatttgcctg atttaaacag cttcgacgga
     8701 gtggtatttg actgcaccaa tacgaccaac ctggatgaga tacagtatat ggtcagaaag
     8761 cttcagtcgg taaatgtgcc ggtagtgagt atcgggtata aggttgatgg gttttactat
     8821 gtaggcaacg ataataagag gctgtttcgc aaggtaatgg atcatatgta ctatgcgcat
     8881 ggctgcaaaa gctttgtgtt tgcaggtggt ccgccgtatc attatgagaa cagaatgcgt
     8941 tttgaggcct tcaaggaggc tttaagtgat tatggcatac cacttacagc tgatatgtac
     9001 atgtttggtg attttgatta tcagacaggc gtcaggtata tgagtgattg gcacgagagc
     9061 aaaaagccgt taccggatgt ctttttatgt gcaaatgaca atatagcagc aggactgtgt
     9121 gcgacagcag aggttcttgg atacaaggta ccacaggatt ttaaggtcac aggctttgac
     9181 aatctggata aggctgcgta ttttaaccca cagattacaa cagtggataa caacaggggc
     9241 aatataggca gaaatgctct tgagatattt aaggcgcttt ggaatggcac gggcgatgct
     9301 tctgataaat accttgattc tgagtttatc ccggctgaga gctgcggatg ccctaatacc
     9361 ggcagagtgg attatcgtaa ttacataaag aatattatca aaggctccgt tgcaagagag
     9421 caggaggagg atgcagtaat gatcctccag aaagagctcg aggagtgcaa tgaatactat
     9481 gatttgtttg agagatactc tgactatata cagagcatga agtgtgacgg agtgtatgtg
     9541 gtcggtgttt ctgatctggc agctgcaagg aacaatgcac actttaggaa gcatggctat
     9601 gatattgacg acgaggttgt actctatgcg gatgacaagg acaatggaaa gcttgagttt
     9661 aagagtgtaa atgaccttat gcagtacatg cagagtgtag ataaaaacac ctgctatatg
     9721 tattgttcgc tgcatttcag ggacgagata gtgggctatg taatactcag gaatcctgag
     9781 tttttgtacg accatcctga gcagtttgat atacagtctg ccctgctcaa gaggcttgag
     9841 aatcttttta agcaaaaggt acttgagaat acaaataatg agcttaagaa tctctacaac
     9901 catgatgcgc tcacaggcct atacaatcgt gtggcatgca atgagatggt cattcctatg
     9961 tttgctgagc ttgaagctca aaatgtcggc tgcaccatag tatttctcga tgtggatgat
    10021 ttcaaggaca taaatgatac atacggacac cagtacggtg acgagctttt aaagactgtg
    10081 gcgagggtgc ttgatgagca aaagcctgag ggaagtatgg tatatagatt cggtggagat
    10141 gaatttatcg tgtttatccc tggggacaga catgacactg ctgagaagta tataaagcgc
    10201 gtgtatgata tcatggagca gcacagcctg tttatcagtc acggcatcat atatacgagg
    10261 ccgggcagtg gtaagagctt tgacgattat ctagtaagtg ctgacacaaa gatgtatcag
    10321 cttaagcagc agcgcaagca gaaaaaggat agcaatttct taaagggagt ggatatttca
    10381 tccgttccta tgatggttga caataatata caaataatgg acagggaagg tcatgtgtgc
    10441 agggtatttg attttttaaa gctgaataat gtaaattcag tccgtctgcg catatggaat
    10501 gagcctgata aggtgccgga gtctaagggg tattgcagtc ttacatatgt tctcgagatg
    10561 gctcatatca ttaaaaaata caaaatgcat tttctgcttg attttcatta ttcagattac
    10621 tgggcagatc cgggacagca gaataagcct gatgcgtgga agaatttaag ctttgatgag
    10681 ctgaaggagg cagtgcacaa gtacacatat gatgtgttat acagattaaa aatcatggat
    10741 tgtgcgccgg atatggttca gattggcaat gaaataagga gtggtatgct gtttccggat
    10801 ggtgcagtgc cacagtacag gcagctggct gcgcttgtaa atgagggaat cagagctgtc
    10861 agggagctgc tcccggagac aaaggtcatg attcatctgg atcagggagg cagatataac
    10921 tgtattatgc cttggtttga ttctatgttt aatgcgggca tgctgccgat tgacgcgatt
    10981 ggcctttcct tttattcgtt ctggcatggt acattcatgg atttgaagga tactatgagc
    11041 aggctcataa aattatataa tctgccggtt tatattgtgg agacagctca tccgtggagg
    11101 cattgcaagg gagatcatat cccgaaggaa ctcatggaga ctgcaggcct tgatgcaggc
    11161 agcgcagagc agaagaagtc tcttgaaata atcatgcaga tagcagcaga ggtgtcaaag
    11221 gataccggaa agacaggtgt atactactgg gagcctgtcg gtgtgcctgg taaaggcatg
    11281 ggaacctggt ttgagaatat gggtatgttt gacgagcatg gcagggcttt gccgggctgg
    11341 gatgcaatca gggactttga tccgaaaaat ccgccgatta aagagcttga caagtatatt
    11401 gaatcacttt atgagtatga agaaacaccg gaggtggagg atttcatgaa gcttcttatg
    11461 attcatggaa atctaatctc aaatcctgaa tttaaggatg gatttaataa ttggcagatt
    11521 gagacaagcc tcgaagaagg acagtataca ctgggtaagg atggagtgtt tatctcttca
    11581 gatgccaatt ttgactattc gat
//
LOCUS       DPUF01000119            3333 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10238, whole
            genome shotgun sequence.
ACCESSION   DPUF01000119 DPUF01000000
VERSION     DPUF01000119.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10238"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="DHV81_04445"
     CDS             complement(<1..694)
                     /locus_tag="DHV81_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HCI93744.1"
                     /translation="MNETTRQLIVHKVKHNTKRLITSIKWVIFSIVVGIVAGSCGTAF
                     YFALSVVTDFRMKYPWLIYFLPIGGLAIVGLYHLCKDDNDTGTNLVISAIHSGDKVPF
                     IMAPLIFVSTLITHLFGGSAGREGAALQMGGSIGSSIGRVFRFDEKDKHVMIMCGMSA
                     AFSALFGTPMAAAIFSMEMISVGVMYYIALVPCVISSLIAHGIASYFNVTNEFFAIEN
                     IPDFTILNSVKIS"
     gene            895..2469
                     /gene="malQ"
                     /locus_tag="DHV81_04450"
     CDS             895..2469
                     /gene="malQ"
                     /locus_tag="DHV81_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCI93745.1"
                     /translation="MRTSGVLMPISSLPSQYGIGTMGKEARRFVDFLEKGGQTYWQIL
                     PICPTSYGDSPYQSFSSFAGNPYFIDLELLCKDKLLTKKECESYKWGKKPQYIDYGIM
                     YVNRYALLRKAYERFSKKTPADYEAFCSKEAEWLDEYTLFMALKDANGGVAWSEWDDA
                     LKLRKPEAMEEAKEKYADDIAFYKMLQYLFFKQWTALKAYANEKGIRIIGDVPIYVAM
                     DSADVWANPTQFYLDKDLNPIEVAGCPPDAFSADGQLWGNPLFRWDVMKKDSYSWWTK
                     RISAMAKLYDIVRIDHFRGFDSFYAIPAKDDTAKNGVWKDGPGMDLFNVLEKKLGKLP
                     IIVEDLGFLTPSVKKLLKDSGFPGMKVIQFAFDSREDSDYLPHNYPQHCVVYTGTHDN
                     DTVMGWMKTAPKDCVRFAKDYLNLTKEEGYNWGMMRAAWSSVADMAIVPMQDLLGLDS
                     KARINIPSTTGGNWQWRATPEQIDNKLAKKLHKCMQMYARLREEPEEASDKSDAKETA
                     DASTGKTAGCEKKAAK"
     gene            2609..>3333
                     /locus_tag="DHV81_04455"
     CDS             2609..>3333
                     /locus_tag="DHV81_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HCI93746.1"
                     /translation="MGSNMDSKEFDLYKDIRCRTNGEIYIGVVGPVRTGKSTFIKHFM
                     ELCVIPKMDNEYAKKRAVDELPQSGKGKTIMTTEPKFIPQDAVTISLDDGSAVKVRLV
                     DCVGFTVGDAVGYLEEDGERMVKTPWFDEDIPFEEAAVVGTKKVIEEHSTVAVLVTTD
                     GSIGDIKRQSYEAAERETVMQLEASGKPFVIVVNTTKPFAAETRLLCESLCRDYKAAA
                     IPIDCEKLCMGQITDIMEKLLYE"
BASE COUNT         1018 a          628 c          796 g          891 t
ORIGIN      
        1 cagagatttt tactgagttt aaaatagtaa agtccggtat gttttcaata gcaaaaaatt
       61 catttgtaac attgaagtat gatgcgatac cgtgagcgat gagggagctt atcacacatg
      121 gaacgagtgc tatataatac atgacaccga cgcttatcat ctccatggag aaaatagcgg
      181 ctgccatcgg agtgccaaac agtgcactga atgcagcgct catgccacac ataatcatga
      241 catgcttgtc cttttcatca aatctgaata ccctgcctat agagcttccg atgctgcctc
      301 ccatctgcag tgcagcaccc tcacggccgg cagagccacc gaacaggtga gtgataagtg
      361 tggatacaaa tataagcggg gccattataa agggcacctt gtcgcctgaa tggatagctg
      421 atattaccag attggttccg gtgtcgttat cgtctttaca cagatgatat agtcctacaa
      481 tggcaagtcc accgattggc agaaagtaaa tgagccaagg gtatttcatt ctgaaatcgg
      541 ttacgaccga cagggcaaaa taaaatgcgg tgccgcagct tcctgcgact atgccgacaa
      601 cgatagaaaa gattacccat ttaatggatg ttataaggcg tttcgtatta tgctttacct
      661 tatgcacaat caattgcctg gttgtttcat tcattatgtt acacctcttt taaacttttt
      721 attaatttga tattaacatt aactaattta atttacaagt tgcactttat gatgttataa
      781 taactaaata aaacgaaaat atcatatgat ttcatatgta tattatatat tattttaaaa
      841 tacaggctca cgcagccaaa caatgagtat acaacaaaag ggaggaaaaa taacatgaga
      901 actagcggtg tattaatgcc aatctcatca ctgccatcac agtatggtat tggaactatg
      961 ggaaaggagg cgagacgttt tgtcgatttc cttgagaagg gaggccagac atactggcag
     1021 attcttccaa tctgtcctac cagctatgga gattctccat accagtcatt ttcaagcttt
     1081 gcaggcaatc cttattttat cgatctggag cttttatgca aggacaagct tctgacaaag
     1141 aaggagtgcg agtcatataa gtggggcaaa aagccacagt atatagacta tggcatcatg
     1201 tatgtgaaca gatatgctct tttaagaaag gcatatgagc gtttttcaaa gaaaacaccg
     1261 gctgactatg aagcattctg cagtaaagag gcagaatggc ttgatgagta cacactcttt
     1321 atggcattaa aggatgccaa tggcggagtg gcatggagcg agtgggatga tgctctcaag
     1381 ttgagaaagc ctgaggctat ggaagaagca aaggagaagt atgctgatga tattgccttc
     1441 tacaagatgc tccagtatct cttcttcaag cagtggactg ctttaaaggc atatgctaat
     1501 gaaaagggca taagaattat cggagatgta cctatttatg ttgccatgga ttcagcagat
     1561 gtatgggcta atcctacaca gttctatctc gataaagact taaatccaat cgaggtggca
     1621 ggatgtcctc ctgatgcctt ctcagctgac ggacagctct ggggcaatcc tcttttcaga
     1681 tgggatgtta tgaagaagga cagctacagc tggtggacaa agcgtatcag tgctatggca
     1741 aagctttatg atatcgtgcg aatcgatcat ttcagaggtt ttgattcatt ctatgctatt
     1801 cctgcaaagg atgatacagc aaagaacggt gtatggaaag atggacctgg catggattta
     1861 ttcaacgtgc ttgagaagaa gcttggcaag cttcctatca tcgtggagga cctcggcttc
     1921 ctcacacctt ccgtaaagaa gcttttaaag gattcaggct tccctggcat gaaggttatc
     1981 cagtttgcct tcgattcaag agaagatagc gattatctgc cacataacta tccgcagcac
     2041 tgtgttgtat atacaggtac ccatgacaat gacacagtta tgggctggat gaagacagct
     2101 ccaaaggatt gcgtaaggtt tgcaaaggat tacttaaacc tcacaaagga ggagggctac
     2161 aactggggca tgatgcgtgc ggcatggtca tcagttgccg atatggctat tgttcctatg
     2221 caggatctgc ttggacttga ttcaaaggcc cgtatcaaca ttccttcaac taccggtggc
     2281 aactggcagt ggagagctac tcctgagcag atagacaata agcttgcaaa gaagctccac
     2341 aagtgtatgc agatgtatgc acgtctgaga gaggagcctg aggaggcttc tgacaaatca
     2401 gatgctaaag aaacagctga tgcttctaca ggcaagacag caggatgtga gaaaaaggct
     2461 gctaagtagt ttggtttaaa atactatata ttattacaaa agaccacagg ttatctgttt
     2521 tgaagcctgt ggccttttta tgtgtaataa ttcaaagagc aggtgaatac actttgatta
     2581 tagaataaag tgtatggagg aagcttttat gggcagtaat atggacagta aagagtttga
     2641 tttatataaa gatatcagat gccgcacaaa cggcgagatt tacataggtg tcgtcggtcc
     2701 tgtgcgcaca ggcaagtcca catttataaa gcatttcatg gagctttgtg tcatcccgaa
     2761 gatggacaat gagtatgcta aaaagcgtgc agtcgatgaa ctgccgcaat caggcaaagg
     2821 aaagactatt atgacgactg aaccgaaatt tattccacag gatgcggtta ctatcagtct
     2881 ggatgatggc agcgctgtga aggtacgcct tgtggactgt gtgggcttta cagttgggga
     2941 tgctgtcggt tatctcgagg aggacggcga gcgtatggtc aaaacaccat ggttcgacga
     3001 ggatatacct tttgaggagg ctgcagttgt aggtacaaaa aaggtaatag aggagcattc
     3061 gacagtggct gtacttgtaa ccactgacgg cagcataggc gatataaaaa gacagagcta
     3121 tgaggcagca gaaagagaaa ccgtcatgca acttgaggct tcaggaaagc catttgtaat
     3181 tgtggtaaat actacaaagc cttttgcagc agaaacgaga cttttgtgcg agtcactttg
     3241 cagagattat aaagcggctg ccattccaat agactgtgaa aagctttgca tgggacagat
     3301 tacagatatt atggaaaagc tcttatatga att
//
LOCUS       DPUF01000120            2890 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6696, whole
            genome shotgun sequence.
ACCESSION   DPUF01000120 DPUF01000000
VERSION     DPUF01000120.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6696"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="DHV81_04460"
     CDS             <1..795
                     /locus_tag="DHV81_04460"
                     /inference="COORDINATES: protein motif:HMM:PF06564.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93747.1"
                     /translation="NNKGGVGKTATVQSLASGIVRLNHNLRVLVIDLDPQCNLSSLFG
                     VRDNEYDNIYNAMCKQSGVPVYKCKNGVYAVPGSAQMENIEQHLPGGPSLREQMKSYT
                     VLLGCLQDNDCHDMTGEGLKNVFDDFDYIFIDCPPALSKNTYNALVAASKILIPVQME
                     ALSVKGVSEVLSVMDEVKEFHMNDNLELLGLLPVMVDERTKITKQLSKLLGEKHGDLI
                     LPCRIRRSVKFLEAQAHGQSIFEYAPYSSTGIDYEIAIKRMFNIKI"
     gene            865..1164
                     /locus_tag="DHV81_04465"
     CDS             865..1164
                     /locus_tag="DHV81_04465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93748.1"
                     /translation="MAVAKRNLEEIGESLENMGMNDRPSLLNTEMAKSEEDKNISKGG
                     RKRKSYWTAEERRTRTSVATYLNKEELLLLKMKCLSDDIPQENLLYNILMDYLKK"
     gene            1536..>2017
                     /locus_tag="DHV81_04470"
     CDS             1536..>2017
                     /locus_tag="DHV81_04470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93749.1"
                     /translation="MSEPKGNKNLVWINTPFSLTKLDKQYTLLQQNILMVASTHLQKY
                     VEEYFTEKRVLGDARSDYLFEKGIEHAVMEIPPIKIDIKDFQVSTEFHNYKNLRDTLK
                     NDILNLSVRVKTDSKTEKIQHVFSNIEIPTTQKGYTKSDGEKVERIKGEVILEIDPKL
                     T"
     assembly_gap    2018..2151
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2178..2837
                     /locus_tag="DHV81_04475"
     CDS             2178..2837
                     /locus_tag="DHV81_04475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93750.1"
                     /translation="MKQYLGLVELDEHGNILLDDKGEPVMNKYPKFSQFRKQVLDVVR
                     EDLNRMASRSETDIVFDEFSEEDFIYRNGKHKGDPEYVIFHIKRTDVGINHIGDKDAD
                     IARRLNEKINRNRGKKTSDDATQQGDLFAHVYQNPDKKIEVDTTQGFEQWNQFISMVQ
                     DTSQQALLGRCKFIGIKNDRFCIAASDDDFAALKTSGLERLAQEFFDCVGSFMPVFYR
                     G"
BASE COUNT          962 a          444 c          621 g          729 t
ORIGIN      
        1 aacaacaagg gtggagtagg caagacggct actgtgcagt ctctggcttc tggcatagtc
       61 cggttgaatc acaatctcag ggttctggta atagacttag acccacaatg caacctctct
      121 tcgcttttcg gagtgagaga taacgaatat gataacatat acaacgccat gtgcaaacag
      181 agtggtgtgc cagtctacaa atgtaaaaat ggagtatatg cagttccagg atctgctcag
      241 atggaaaata tagaacaaca ccttccagga ggcccttcac tgagagaaca gatgaagagc
      301 tacacggtat tactgggatg tttgcaagac aacgattgcc atgacatgac aggagaagga
      361 ctgaaaaacg tatttgatga tttcgactac attttcatcg actgtcctcc agcactttcc
      421 aagaacactt ataatgcttt ggttgcagcg agcaagattt tgattcctgt acagatggaa
      481 gcattatctg tcaagggagt aagtgaagta ctcagcgtaa tggatgaagt gaaggagttc
      541 cacatgaacg ataatttgga attacttgga ctcctgccag ttatggtaga tgaacgaacc
      601 aagattacca agcagttgag caaactttta ggcgaaaaac atggcgactt aattctgcct
      661 tgccgtatcc gacgctctgt aaagttcctg gaagctcagg cacacggaca aagcatattc
      721 gaatatgcac cttactcaag tacaggtatc gattacgaaa ttgctatcaa gcgcatgttc
      781 aacatcaaaa tctaatcttg gtagggtagg ttgaaacatg taataataaa acaaaagaag
      841 ataaacttat aaaatataag aattatggct gtagcaaaaa gaaatttaga agaaattggt
      901 gagtctcttg aaaatatggg tatgaacgac cgaccaagtt tattaaacac agaaatggca
      961 aaaagcgaag aggataaaaa tatatctaaa ggtggtcgca agagaaaaag ttactggact
     1021 gcagaggaaa gacgcactag aacgagtgtg gctacatatc ttaacaagga agaactcctt
     1081 ctcctgaaga tgaaatgcct ctcagatgat atcccacagg aaaatctgct ctacaatatc
     1141 ctgatggatt acttgaaaaa gtaagcaaac aaaaagtagt tattaaaagg gtgaaaacca
     1201 caaaactgtt tacctaaaaa agcgctaagc ttaaaaataa ggtataagtt cctacaaaaa
     1261 ccacattttt gtttaccaaa agaatgataa ttatagatta ttctttataa taaattatat
     1321 atctcttatt tattacagaa agcataatag cttgattaat aatgagttat gtttgaaatg
     1381 gtaaatagaa aagtggtttt aggtaaatag gaatatggtt tatggtaaat agcttggtgg
     1441 tttcgggtaa atatttatgt ggttttaagt aaataaaaaa gtggttttag gtaaacaact
     1501 ttgtggttta tggtaaacag aaatgtggtt tttatatgag tgaaccaaaa ggaaacaaga
     1561 atctggtgtg gattaacaca cctttttcgc tgacaaaatt ggataagcaa tatacattat
     1621 tgcaacaaaa catattgatg gttgcaagta cccatctgca gaagtatgtt gaagagtatt
     1681 ttaccgagaa gagagtgctt ggagatgcac gctccgacta tcttttcgag aaaggaatag
     1741 agcatgctgt tatggagatt ccgcccatca aaatagacat caaggatttt caggtatcaa
     1801 cggagtttca taattataag aatctgcgtg atacgctgaa gaacgatatc ctcaacctgt
     1861 cggtgcgtgt aaagacggat agcaagacag agaagataca gcacgtattt tcaaacatcg
     1921 agattccgac aacgcagaaa ggctatacaa agagcgatgg tgagaaagta gaacgcatca
     1981 agggtgaagt aatacttgag atagatccaa aactcacnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggatgtta
     2161 aggttgagtt tttgccgatg aaacagtatt tgggtctggt agaacttgat gaacatggca
     2221 atattctgct tgacgacaag ggggagcctg tgatgaataa atatcctaaa ttctcgcaat
     2281 tcagaaaaca ggtactcgat gtggtaaggg aagatctcaa cagaatggct agtcgcagtg
     2341 agacagatat cgtatttgat gaattttctg aagaagactt tatttatcgg aacggaaagc
     2401 ataagggaga tcctgaatat gttatcttcc atatcaagcg gacggatgta ggtatcaatc
     2461 atatcggaga taaagatgca gatattgctc gtcgtctgaa tgaaaaaata aaccgtaata
     2521 ggggaaagaa aacatctgat gatgccacgc agcagggaga tctttttgcg catgtatatc
     2581 agaatcctga caagaagata gaagtagaca cgactcaagg ttttgagcaa tggaatcagt
     2641 ttatttcgat ggtacaggat accagtcagc aagccctgtt gggcagatgc aaattcatag
     2701 gaatcaagaa tgacagattc tgtattgctg ctagtgatga tgattttgct gcattgaaaa
     2761 catcgggtct tgaaaggctg gcacaagaat tttttgactg tgtcggttca ttcatgcctg
     2821 tattctatag aggctaagca gaacaagaaa gcagaagagg aagtagggga gtaccgtact
     2881 tcctcttttt
//
LOCUS       DPUF01000121            3290 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6516, whole
            genome shotgun sequence.
ACCESSION   DPUF01000121 DPUF01000000
VERSION     DPUF01000121.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6516"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(168..386)
                     /locus_tag="DHV81_04480"
     CDS             complement(168..386)
                     /locus_tag="DHV81_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93751.1"
                     /translation="MFDEEMITKYRYIILLLGTAVSFVFGFAVAFGLAKLCGVMAVKA
                     VLNIAFTAGGITGMLGFVCICLAELRRI"
     gene            784..981
                     /gene="thiS"
                     /locus_tag="DHV81_04485"
     CDS             784..981
                     /gene="thiS"
                     /locus_tag="DHV81_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiS"
                     /protein_id="HCI93752.1"
                     /translation="MVKINGKEAADVCGMTVSQYLSANNYNPKHIVVELNEEILPKTE
                     YDSTAIADGDVVEILSFMGGG"
     gene            1032..1820
                     /locus_tag="DHV81_04490"
     CDS             1032..1820
                     /locus_tag="DHV81_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCI93753.1"
                     /translation="MSKNIDLANDKLVLGGHEFTSRFILGSGKFSLDLVKACIEKADA
                     QIITLALRRANDGGLANILDYIPDNVTLLPNTSGARNADEAVRIARLSRELGCGDFVK
                     IEIMRDSKYLLPDNFETIKATEILAKEGFVVMPYMYPDLNVARDLANAGAACIMPLGA
                     PIGSNKGICTKEFIQILIDEIDLPIIVDAGIGRPSQACEAMEMGAAAVMANTAVATAG
                     DVEIMAEAFKKAIEAGRMAYLSGLGRTLTKGASASSPLTGYLRN"
     gene            1859..3124
                     /locus_tag="DHV81_04495"
     CDS             1859..3124
                     /locus_tag="DHV81_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCI93754.1"
                     /translation="MTNDTNITNDKDINANIAHDDHFNDSIVNEVILEDMKKNRIDHM
                     KYLPDMEVLDSDIMDRVVAEMDDYDYDKYTAADVRAALSHEYRTLEDFKALLSPAAQP
                     FIEEIAQAAQLETRKHFGNSVCMFTPLYISNYCENYCIYCGFNCHNKINRAKLNASEI
                     EKEMAAIAKTGLQEILILTGESKKMSDVEYIGEACKIARKYFKVIGLEVYPMNSDEYA
                     YLHECGADYVTVFQETYNSDKYETLHLAGHKRIFPYRVNAQERALKGGMRGVGFAALL
                     GLDDFRKDALATGYHAYLLQKKYPRAEIAFSCPRLCPIINNDRINPKDVHETQLLQVV
                     CAYRLFMPFASITISTREVARVRDNLVNIAATKISAGVSTGIGSHVDDIEDKGDDQFE
                     ISDGRSVDEVFNALLDNGLQPVMSDYIYV"
     gene            3133..>3290
                     /locus_tag="DHV81_04500"
     CDS             3133..>3290
                     /locus_tag="DHV81_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCI93755.1"
                     /translation="MNKEKLLDENSKHALQSDNIIAITNRHLCHRPFMEQLERVCKLH
                     PHAIVLRE"
BASE COUNT          978 a          741 c          707 g          864 t
ORIGIN      
        1 gtgtgcgaat atgcactttt tatgtgaaat cagtaactat tcagatccat actcatcctg
       61 cgcatgaatg tccatcttcc tatcggaggc attcacatat ttctttgttc ttttttattt
      121 cgtcgtatgt caaaataatt tattccgttt tctttcttac tgtgtaacta tatgcgtcta
      181 agctcagcca gacagatgca cacaaatcca agcattcctg tgataccgcc tgccgtgaaa
      241 gcgatattga gcactgcctt tacagccatc actccacaca gcttagcaag tccaaatgcc
      301 accgcaaagc caaacacaaa cgatactgct gtgccaagaa gtaatattat gtatctgtat
      361 tttgttatca tttcttcgtc aaacattatt ttcctccatc aaatcggcta tcagaaatta
      421 ttcaatatca tttttattac ttttttaaca tatcattcta atgaaaatat actgtctgcg
      481 tcatgtaaaa tttgttggaa attgttgtaa tgcagttact acatatcagt aacgtaatgc
      541 ggttttatta aaaatcagtt gactttgccg gtgcaaggta ctatactcat gtttgtaaaa
      601 ttattggtat ctccctttta attttacata tttacatagt ttaataacgg atttttgaga
      661 gtgaacagag ttcatgaagg ggtacaccac cgcgggtgta ttgctttgtg aactcttttt
      721 taatgtattg cttcgggata atccccgctt tttccacatc tcaatccgga aaggagacgc
      781 acaatggtaa aaatcaacgg taaagaagct gcagatgtat gcggcatgac tgtttcgcag
      841 tatctttcag ctaacaatta caaccctaag cacatcgttg tagagctgaa tgaagagatt
      901 cttccaaaga ctgagtatga cagcactgct atcgccgacg gcgatgtggt agagatttta
      961 agctttatgg gcgggggctg attaaaaaca aacacttttt acaccatcaa taaaaatata
     1021 aggagttcat catgagtaaa aatatagatt tagcaaacga caaattagtt ttaggaggtc
     1081 acgagtttac ctcacgtttc atccttggat cgggaaagtt ttcactcgac ctggtaaagg
     1141 cctgcattga gaaggctgat gcacagatta tcacactggc tctgcgccgt gcaaacgatg
     1201 gaggactggc aaacatcctc gactatattc cggacaatgt cacactgctt ccaaacacat
     1261 caggcgccag aaatgccgat gaggctgtaa gaatcgcacg tttgtcccgc gagctgggct
     1321 gtggagattt cgtaaaaatt gaaatcatgc gcgacagtaa atatctgctt ccggacaact
     1381 ttgagacaat aaaggctaca gagattctcg caaaggaggg ctttgtcgta atgccttaca
     1441 tgtatcctga cctcaacgta gcaagagacc ttgcaaacgc cggcgctgcc tgcatcatgc
     1501 ctctcggtgc gcctatcggc tcaaacaagg gtatctgcac aaaggaattt atccagattc
     1561 tgattgacga aattgacctg cctatcatcg tggatgcagg cataggccgt ccttcacagg
     1621 catgcgaggc tatggaaatg ggtgctgccg cagtcatggc aaacaccgct gttgcaacag
     1681 ccggtgatgt tgagataatg gcagaggcat ttaaaaaggc tatcgaggcc ggaagaatgg
     1741 cttatctgtc aggactcgga cgcactctga ccaaaggagc cagcgcatca tctcctctga
     1801 caggatatct gcgcaactaa tcgattatat aataattagc ttttgaaggg aacacataat
     1861 gactaacgat acaaatataa caaatgacaa agatatcaat gcaaatatcg cccacgatga
     1921 ccacttcaac gacagcattg taaacgaagt aatcctcgag gacatgaaga aaaacagaat
     1981 agatcatatg aaatatctgc ccgatatgga ggtgcttgac tccgatatca tggacagggt
     2041 agttgccgaa atggatgact acgactacga caaatacacc gcagccgatg tacgcgcggc
     2101 gctctcccac gaataccgca cattggagga tttcaaggct ctgctctcgc ctgctgcaca
     2161 gccatttatt gaggaaatcg cacaggctgc acagcttgag accagaaagc acttcggcaa
     2221 cagcgtgtgc atgtttacac cgctttacat atcaaactac tgcgaaaact actgtatcta
     2281 ctgtggtttc aactgtcaca ataaaatcaa ccgcgcaaag ctgaatgcct cagaaataga
     2341 aaaggaaatg gctgcaatcg ccaaaaccgg tcttcaggaa atcctgattc tcaccggtga
     2401 gagcaaaaaa atgtcagacg tcgaatacat tggagaggcc tgcaaaattg cccgcaaata
     2461 tttcaaggtt ataggtcttg aggtttatcc tatgaactct gacgaatacg catacctgca
     2521 cgagtgcggt gctgactatg tgaccgtttt ccaggagacc tacaactctg acaaatacga
     2581 gactcttcac cttgccggac acaagagaat tttcccttac cgtgtaaatg cacaggagcg
     2641 tgctcttaag ggcggtatga gaggtgtcgg ctttgctgca cttttaggtc tcgatgattt
     2701 caggaaggat gcgcttgcca ccggatatca cgcatacctg ctccagaaaa aatatccaag
     2761 agccgagatt gcattctcat gccctcgtct gtgccctatc ataaacaatg accgtatcaa
     2821 tccaaaggat gtccacgaga cacagctcct tcaggtggta tgcgcttaca ggctcttcat
     2881 gccgtttgca agcatcacca tatctaccag agaggtagcg cgtgtacgtg acaatctcgt
     2941 aaatatcgcc gccaccaaga tttcagccgg tgtcagcacc ggaatcggaa gccatgtgga
     3001 cgatatagag gacaagggcg atgaccagtt tgaaatctct gatggcagaa gcgtggatga
     3061 ggtatttaat gcactgctcg acaatggttt acagccggtg atgagcgact atatttacgt
     3121 gtaggtgcga atatgaataa agaaaaactc ttagatgaaa actcaaaaca tgctttacag
     3181 agtgataata taatcgctat cacaaacaga cacctgtgcc accgcccatt catggaacag
     3241 ctcgagcgcg tctgtaagct tcatcctcat gcaattgtgc tcagggaaaa
//
LOCUS       DPUF01000122            3185 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_20716, whole
            genome shotgun sequence.
ACCESSION   DPUF01000122 DPUF01000000
VERSION     DPUF01000122.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_20716"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            217..1248
                     /locus_tag="DHV81_04505"
     CDS             217..1248
                     /locus_tag="DHV81_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCI93756.1"
                     /translation="MSFTFLNQLPTSAQIKEEYPLSKELTELKAKRDAMISDVICGKD
                     DRFLVIIGPCSADNEDSVCDYVSRLTKIQEDVKDRVIIIPRVYTNKPRTTGEGYKGIA
                     SQPDPEKAPDMVEGLIAMRKMHIRAIAESGLTCADEMLYPENWGYVEDLLSYVAIGAR
                     SVEDQQHRLTVSGFDVASGMKNPTSGDFSVMLNSVYAAQHPHHFVYRGYEVETSGNPL
                     THVVLRGAVSKHGNTTTNYHYEDLIRLHEMYDKMDVVNQAAIIDTNHSNSGKKFKEQI
                     RIAKEVMHNRQLSSDIKSLVKGLMIESYIEEGSQKIGEHVYGKSITDPCLGWEDSKKL
                     IYDIAEMNS"
     gene            complement(1331..1870)
                     /locus_tag="DHV81_04510"
     CDS             complement(1331..1870)
                     /locus_tag="DHV81_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCI93757.1"
                     /translation="MIDEMKRQKRELVYKGSILDIYKDTMQFANGKTEEWDFVSHRKG
                     AAAVLPVLDDGRIIMVRQYRNALERETIEIPAGCRDFVGEDTRLCAERELKEETGYSS
                     DDISFLLSLRTTVAFCDEEVDVYLAKNLKKGEQHLDDAESIDVEIYTLKELCDMIYAG
                     KIQDSKTVSAVLAYSNMIK"
     gene            complement(1920..3137)
                     /locus_tag="DHV81_04515"
     CDS             complement(1920..3137)
                     /locus_tag="DHV81_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014120145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butanol dehydrogenase"
                     /protein_id="HCI93758.1"
                     /translation="MQRFTLPRDLYHGKGALEALKSFEGKKAIICVGGGSMKRFGFLD
                     KAEAYLKEAGMEVKLFEGIEPDPSVETVMKGAAAMAEFEPDWIVAIGGGSPIDAAKAM
                     WIKYEYPDITFEDMCKVFGIPKLRKKAHFCAISSTSGTATEVTAFSIITDYEKGIKYP
                     IADFEITPDVAIVDPELAETMPQKLVAHTGMDAMTHAIEAYVSTANSDFTDPLALHAI
                     EMIQHDLIDSYNGDMAKRDAMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFADYGAHI
                     IHGAANAMYLPKVIAFNAKDEAAKERYGVIADFMKLGGETLDEKVELLIKYLRGMNDD
                     LNIPHCIKNYGADSYPTEQGFVPEEVFLERLPEIAANAILDACTGSNPRQPSQEEMEK
                     LLKCCYYDTEVDF"
BASE COUNT          861 a          749 c          613 g          962 t
ORIGIN      
        1 ctttttttca atgttacttt tttattattg tagtctttta gtgttttatt ataacttatt
       61 atgcatacta ttataattga taaaaatttg acaatatatt tcatttatct tattatttaa
      121 ctgtttgggc attatatatt ggaaattttt tccgatatgc cataaaaata taccatttta
      181 attaatacaa ttactattta tataaggagc agtattatga gtttcacatt tttaaaccag
      241 cttccaactt ctgcacagat taaggaggaa tatcctctgt caaaggagct taccgagctt
      301 aaagcgaagc gcgatgcaat gatttcagat gttatctgcg gaaaagatga tagatttctc
      361 gtcattatcg gaccttgttc agccgacaac gaggattcag tctgtgacta tgtcagccgt
      421 ctgacaaaga ttcaggagga tgtaaaggac cgcgtaatca tcatccctcg tgtatataca
      481 aacaagccaa gaacaaccgg cgagggctac aagggaattg cctcacagcc tgaccctgag
      541 aaggctcctg acatggttga gggtcttatc gccatgagaa aaatgcacat ccgtgccatc
      601 gcagagagtg gactcacctg tgcagatgag atgctttacc ctgagaactg gggctatgtt
      661 gaggatcttc tctcatatgt ggcaatcggt gctcgttctg ttgaggatca acagcatcgt
      721 ctcaccgtca gcggcttcga tgtggccagc ggcatgaaaa accctacaag cggtgatttt
      781 tccgtcatgc tcaattctgt ttacgcagca cagcacccac atcattttgt ttacagaggc
      841 tatgaggttg agacatcagg gaatcctctg actcacgttg ttttaagagg tgctgtcagc
      901 aagcatggca acactacaac aaactaccat tacgaggact taatccgtct gcacgaaatg
      961 tacgacaaga tggacgttgt caaccaggcg gctatcatcg ataccaacca ttccaactcc
     1021 ggaaagaagt tcaaggagca gattcgtatc gccaaggaag ttatgcacaa cagacagctt
     1081 tcctctgata tcaaatcact tgtcaagggt cttatgattg agagctatat tgaggagggc
     1141 agccagaaga ttggtgagca tgtctacgga aaatccatca ccgatccatg tctaggatgg
     1201 gaggattcaa agaagcttat ctacgacatt gcagaaatga attcttaaac cacaaattat
     1261 acacacattt tttacaaagt acaagccaca ggagtatata tcctgtggct tgcattattt
     1321 tgtcgattta ttatttaatc atattactat aagcaagaac tgcactgact gttttggaat
     1381 cctggatttt tccggcataa atcatgtcgc acagctcctt tagagtgtat atttctacat
     1441 cgattgactc ggcatcatcc agatgctgct cgcccttttt caggttttta gcaagataga
     1501 catctacttc ttcgtcgcaa aaggcaactg ttgtgcgaag tgaaagtaaa aacgagatat
     1561 catcagagct gtagcccgtc tcctccttaa gctccctttc tgcacacagt ctggtatctt
     1621 ctcccacaaa gtctctgcag ccggccggaa tctctattgt ctccctctcg agagcatttc
     1681 tatactgacg caccatgatt atcctgccgt catcaagcac cggaagcact gccgcagcgc
     1741 cctttctgtg tgaaacaaaa tcccattcct cagtcttacc gtttgcaaac tgcattgtat
     1801 ccttgtatat atcgagtatt gagcccttat atacaagctc tcttttttgc cgtttcattt
     1861 catcaatcat ttttcctgtt taccttctaa ataatataaa ttcagagtta ccgttttgat
     1921 tagaaatcca cctctgtgtc ataatagcag catttgagaa gcttctccat ctcctcctgg
     1981 cttggctggc gaggatttga gcctgtacaa gcatcaagga tagcatttgc agcaatctca
     2041 ggaagtctct caaggaatac ctcctccggt acaaagccct gctctgttgg atagctgtct
     2101 gctccgtaat tcttgatgca gtgtgggata ttcagatcat cattcatgcc acgtaagtat
     2161 ttgataagaa gctcaacctt ctcatcaagt gtctctccac ccagtttcat gaaatctgct
     2221 attacgccat agcgttcctt cgctgcctca tcctttgcat taaaagcaat aacctttggc
     2281 agatacatgg cattggctgc tccgtggatg atatgtgcac cataatcagc aaaagctgcg
     2341 cctgtcttgt gtgccatgga atgaactatt ccaagtagtg cgttggagaa tgccattcct
     2401 gccagacact gcgcattgtg catcgcatct ctctttgcca tatcaccatt gtatgaatca
     2461 atcaggtcat gctgtatcat ctctattgca tggagtgcaa gcggatctgt aaagtcactg
     2521 ttggctgttg atacatatgc ctcgatggca tgtgtcatgg catccattcc tgtgtgtgca
     2581 acaagcttct gtggcattgt ctctgcaagc tcagggtcta cgattgcaac atcaggtgta
     2641 atctcaaagt ctgcgattgg atacttgata cccttctcat aatctgtgat gattgagaac
     2701 gcagtaacct cagttgctgt acctgatgtg gatgaaatag cacagaaatg tgccttcttt
     2761 ctaagctttg gaataccaaa taccttgcac atatcctcga atgtgatatc cggatactcg
     2821 tacttaatcc acatagcctt agctgcgtct ataggtgagc caccaccaat tgctacaatc
     2881 cagtccggtt caaactcagc catggcagcc gcacctttca tgactgtctc aactgacgga
     2941 tccggctcaa ttccctcaaa aagctttacc tccatacctg cttccttcaa ataagcctcg
     3001 gctttgtcta aaaagccaaa tctcttcatt gagcctccac ccacacagat aatagccttc
     3061 tttccctcaa agctctttaa tgcctcaagc gcaccctttc catgatataa atctcttggt
     3121 aatgtaaatc tctgcattgt aaaaccctcc atatgatatt ttttatatag tttctccctt
     3181 gtaat
//
LOCUS       DPUF01000123            9776 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_894, whole
            genome shotgun sequence.
ACCESSION   DPUF01000123 DPUF01000000
VERSION     DPUF01000123.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_894"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1931)
                     /locus_tag="DHV81_04520"
     CDS             complement(<1..1931)
                     /locus_tag="DHV81_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecQ"
                     /protein_id="HCI93759.1"
                     /translation="MNINQTLKQYFGYDSLRTGQEELINGILAGHDVLGIMPTGAGKS
                     LCYQLPALMLKGITLVISPLISLMSDQVKALNQAGVHAAYINSSLTENQIRMALSYAS
                     QGRYKIIYVAPERLNTPRFLDFACNADISMLTVDEAHCISQWGQDFRPSYLEIAGFLT
                     RLPRRPIVSAFTATATERVKNDIVASLGLNNPVTMVTGFDRPNLFFRVVTRKGGSQKD
                     NSIINYVKKHEDESGIIYCATKKNVDKLYTLLNEQGISAGRYHAGLSNDERKQNQEDF
                     TYDRIRVMVATNAFGMGIDKSNVRYVLHYNMPQSLEYYYQEAGRAGRDGEEAECVLFF
                     SKQDIMINKFLLQNKASAGDVASDIQKTANDQRKLQQMINYCETDKCLREFILSYFGD
                     TTPCICNKCSNCVVVEDEEEETYVETGKKRKNAAQLAGLNELGAALFEKLRSVRTELA
                     AEKSVPPYIICSDKTLKDMCAKLPRDKEQLADVYGMGEQKIQNYGEAFVTAVNSFVAD
                     NPNPSGSTTGERPQTVLSDEEAAETGSTRKKKIPFYIGPQRLDEVELTDKCRLTELTN
                     KINELCPADKEHKKLAASFINELLIAEGYLEEVTEGENKIKRVTEKGRSVGIDEEERK
                     AKFGGSYYAITHSKQSQQV"
     gene            complement(1928..4066)
                     /locus_tag="DHV81_04525"
     CDS             complement(1928..4066)
                     /locus_tag="DHV81_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HCI93760.1"
                     /translation="MKQKLSVAPTLKNYDKKNLPKDILAGIIIMAVSIPISMGYAQIS
                     GLPAVYGLYGSVFPIILFALFSTSPQFIFGVDAAPAALVGAAVLGMGIEAGSKEAMTV
                     VPVFTFFVALWLLAFYFMNAGKLVNYISAPVMGGFITGICTTIILMQAPKLMGSAAGT
                     GELFELSEHIWEALHHINAAALVMGIVALAILVVSKRLVPKFPMAVVLMVTGALFTYF
                     GPVKEWGVPTLSAVEPGMPRWYIPDFEAVFSVQKASEVIVLSLSVAVVIMAETLLAEN
                     NFAQKNGYRIDDNTELLAFSIGNMAAAFTGCCPINGSVSRTAMSEQYEGKTQLTGLVA
                     GVSMIAVLLFCTGFIGYLPVPVLTAIVISALMGATEFHLAKRLWKVSRTEFFIFVGAF
                     FGVLILGTINGVLIGIILSFAEMIIRSAKPATCFLGVQPGHSHFRDIRESTNIHEIDG
                     VIIYRFSSGLFFANAKVLVRDIEDHLKHDTKAVIIDAGAIGSIDITGADSIESLYRSL
                     KQKGVKLYITEHIAELNEQLRKLGLGYLIEQGCVRRTIHIALKDMGINRPYPLEGGVD
                     NEERSASRKRADNRVQEFVWAFGAESEEQIEKQIKLQIEQLKKTKDIEEIMHGRWAHM
                     DEFDQDEWLEHLEEHLKEIVNISGKDVHTLAADIEMHRREVHERIAREHPELAERFAQ
                     RRHLLDKHLKERRPEVYRIIVQLREDNKHK"
     gene            complement(4067..5764)
                     /locus_tag="DHV81_04530"
     CDS             complement(4067..5764)
                     /locus_tag="DHV81_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93761.1"
                     /translation="MVKTIGIGKQDFASVIEDNCFFVDKTSFIKEWWDSKDDVTLITR
                     PRRFGKTLNMSMLNCFFSNQYKDRSDLFDSLSIWEDEDYRKIQGTYPVIFMSFASVKA
                     DNLGDAKIQIKAQIEAVYKRHRYLLEGDTLTADEIADFEGTNTRMGDAESGLKLNILC
                     EYFHRYWGKKTIIILDEYDTPIQEAYLHGYWNEFTAYIRSLFNATFKTNPYMERAIMT
                     GITRVSKESIFSDLNNLNVITTTSSSYATSFGFTENEVFDAMEEYGLSGEKDTVKKWY
                     DGFKFGNHSDIYNPWSITNFLKEKQISPYWASTSSNGLVNRLIRTASAEIKSMMETLL
                     EGDSVEVSIDEQLVFEQLDNDENAIWSLLLASGYLKVDSLEKRGLTLEPWYHLSITNL
                     ETVSMFSNMFRGWFNASVSNYNGFIRALLRGNVKEMNIYMNEVALSTFSSFDSGKHPS
                     GKSEPERFYHGFVLGLLVELRDKYEVQSNRESGYGRYDVMIIPRDKNSQAVIIEFKVI
                     DSQEETSLDMTADSALKQIEEKNYDAKLLERGYKREAIQHYGFAFEGKKVLIKKAQI"
     gene            complement(5919..6866)
                     /locus_tag="DHV81_04535"
     CDS             complement(5919..6866)
                     /locus_tag="DHV81_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCI93762.1"
                     /translation="MDVVALGELLIDFTENGTSAQDNKLFEANPGGAPCNVLAMLQKL
                     GHQTAFIGKVGQDAFGRLLVDAVKEQGIDTTGVRYDDNVHTTLAFVQTAADGDRDFSF
                     YRNPGADMMLTADEVDLSLVRNAKIFHFGSLSMTDKICENATKHAIAAAKEAGVLISF
                     DPNLRKPLWKSMDDAKEKISWGLSQCDILKISDDEIEFMTGEKDIKTGVKKLIDEYHI
                     PFICATMGKNGSMAFFDGHIVEAAPFLRDDTVETTGAGDTFCACLLHDVLEHGINDRK
                     DDDVKKMLTFANAAASLITTRKGALRVMPEKGEVEAVIK"
     gene            complement(6989..8452)
                     /locus_tag="DHV81_04540"
     CDS             complement(6989..8452)
                     /locus_tag="DHV81_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCI93763.1"
                     /translation="MSELLEKARSYEAKKIAATDKESKPLFHISAPAGWINDPNGFSV
                     YNGKIHLFYQYYPYLREWGPMHWGHSTTSDMIKWEQLPCVMAPDEEYDKKGCFSGSAI
                     EADGKHVLVYTGVTSIKLPDGSEQERQNQCIAFGDGLNYVKHENNPVVTGDMLPENCS
                     RIDFRDPKIWKEDDTYYLIVGSKDLNNVGQVVLCSSKNLTDWQFETILAANSTGKIGT
                     MWECPDFFGLEDKHVLICSPQSMKADKYEFHNGHNSVYFLGDYDKENHVFIKEEPHTL
                     DYGMDFYAPQTTLLPDGRRVMIAWMKSWDACVVPDSQDWQGMMTLPRELEIKDGRIWQ
                     KPVKEIENYRANKCHYTDKKIDCYTTFDGIKGRTLDMTVELSGEEYNNFTVEMACDDE
                     YGTVFTYNRVAGVLEIDRTCCGVTKDVVCVRKIKIVDTDRKLKLRFIMDRQSIELFIN
                     DGQQVATTAICTPLQADGICFNCDGSAVVDIEKYDII"
     gene            complement(8508..>9776)
                     /locus_tag="DHV81_04545"
     CDS             complement(8508..>9776)
                     /locus_tag="DHV81_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI93764.1"
                     /translation="KYMPNLQAVFEKYPEYRTMCTAPDGHIYSFPWIEQLGAGKEAIQ
                     AIGDIPYINKKWLDYLGLEVPTTTDELEQVLIAFRDHADELEKEFDIEGGVIPMSFII
                     NNGDQDPAILMNGFGEGYGDTGDHFAVTDEGKVIYTPTQEGYKEGIEWLHKLVTEDLI
                     DPEAFTQEWSTYVAKGKNHRYGLCFTWDIANIDNNTDYVMLPALTGPDGVRNITRQNN
                     SETSGFDRGRCVLTSSCRDTALAAAWIDQMYAPIQSPQNNWGTYGEKDSFNIFEMSTN
                     ADGGQMLKHMDLGDQSPVEVREAQSVNGPLAVLNEYYDVYVTEPADAKWRLDNMHEAY
                     LKDMNSKYVYPNVFMSIDDTNKVSQYDTDIKKYAEQKKADWILNGGIDKEWDSYLKKL
                     DKYGLQDYLKIKQKYFDSYQKSLESTSEGK"
BASE COUNT         2453 a         2527 c         1808 g         2988 t
ORIGIN      
        1 atcacctgct ggctctgctt cgagtgggtg atagcgtaat agcttccacc aaattttgcc
       61 ttgcgctctt cctcatctat tcctacgctt cgtccctttt cggtgacacg ctttatcttg
      121 ttctcgccct ctgtaacttc ctccagatat ccctctgcaa taagaagctc gtttataaac
      181 gaggctgcaa gctttttgtg ctccttatcc gcagggcaca gctcatttat tttgttcgta
      241 agctctgtaa gcctgcattt gtcggtgagc tctacctcat caagtctctg tggcccgatg
      301 taaaacggaa tcttcttttt tctcgtgctg ccggtttcag cagcctcctc atcgcttaaa
      361 acagtctgtg gtctttcgcc ggtcgtgctg cctgatggat ttgggttatc tgcaacaaat
      421 gaatttaccg ccgtcacaaa cgcttcacca taattttgta tcttctgctc acccattccg
      481 tacacatcgg caagctgttc cttatcccgt ggcagctttg cgcacatatc ctttaaggtc
      541 ttgtcggagc agatgatata cggaggcacc gacttttctg cagcaagctc tgtacgcacg
      601 ctgcgcagct tttcaaacag cgcagcacca agctcgttaa gccctgcaag ctgtgctgca
      661 tttttgcgct ttttgccggt ttcaacgtat gtctcctcct cttcatcctc cacaaccaca
      721 cagttgctgc atttgttgca gatgcatggc gttgtgtccc cgaaatatga aagtataaac
      781 tctctcaggc atttgtccgt ctcacagtaa tttatcatct gctgcaactt tctttggtca
      841 ttggctgtct tttgaatgtc agatgccaca tcgcctgctg aagccttatt ctgcagaagg
      901 aacttgttta tcatgatatc ctgctttgag aaaaacagca cacattccgc ttcctcaccg
      961 tctcgccccg ctcttccggc ctcctgatag taatactcga ggctctgtgg catgttgtaa
     1021 tgtagcacat atctcacatt tgacttgtcg attcccattc caaatgcgtt tgtggcaacc
     1081 atcacacgga ttctgtcgta tgtaaaatcc tcctgattct gcttacgttc atcatttgaa
     1141 agccctgcgt ggtaacggcc cgccgaaata ccctgctcat ttaaaagagt atacagctta
     1201 tccacattct ttttcgttgc gcagtatatt attccacttt cgtcctcatg ctttttcaca
     1261 taattgatta tactgttgtc cttctggctg cctccctttc ttgtaacaac cctgaaaaac
     1321 aggttaggtc tgtcaaaccc agtcaccata gtaaccggat tgttaagtcc aagcgaagcc
     1381 actatatcat ttttcacgcg ctccgtggcg gtcgctgtaa acgcactgac tatcggtcgt
     1441 cttggaagtc tcgttaaaaa tccggcaatt tccagatagc tcggtctaaa atcctgtccc
     1501 cactgcgata tacagtgtgc ctcatcaact gtcagcatcg atatgtccgc gttgcaggca
     1561 aaatccagaa aacgcggcgt gttgaggcgc tccggagcga catagattat cttgtatcgc
     1621 ccctgtgagg catacgaaag tgccatcctt atctggtttt cggtcagtga gctgttgata
     1681 taggcggcat gcactcctgc ctggtttaaa gccttgacct gatctgacat aagcgagata
     1741 agtggcgaaa tgacaagcgt aattccttta agcataagcg ccggaagctg gtagcacagc
     1801 gattttcctg cgcctgtcgg catgataccg agcacatcgt gccctgcaag tatgccgttt
     1861 atgagctcct cctgccctgt tctaagactg tcgtagccaa agtattgttt gagtgtctgg
     1921 tttatgttca tttatgcttg ttgtcctccc ttagctgcac gataatcctg tagacctcag
     1981 gccttctctc cttcagatgc ttatcaagca ggtgtctcct ctgtgcaaac cgctctgcaa
     2041 gctccggatg ctctcttgct atacgctcat gcacctcgcg acggtgcatc tcgatatctg
     2101 ccgcaagcgt gtgcacatcc tttcctgata tgttgacgat ttctttcaga tgctcctcca
     2161 gatgctccag ccactcatcc tggtcaaact catccatatg cgcccagcgg ccatgcatga
     2221 tttcctctat atcctttgtc tttttgagct gttctatctg aagctttatc tgcttttcaa
     2281 tctgctcctc actctcggcg ccaaacgccc agacaaactc ctgcactctg ttgtcagcac
     2341 gctttcttga ggcactgcgc tcctcgttat ctacaccacc ctcaagcgga taaggtctgt
     2401 ttattcccat atcctttagc gcaatatgta ttgtccttct gacacagccc tgctcaatta
     2461 aataacctaa gcccagcttc ctgagttgct cgttgagctc tgcaatgtgc tctgttatgt
     2521 atagctttac acctttttgt ttcagtgagc ggtaaagtga ctctatgctg tcagctcctg
     2581 tgatatcaat acttccgata gctccggcat cgattatgac agcttttgta tcatgcttta
     2641 ggtggtcctc aatatcacgc acaagtacct ttgcatttgc aaaaaacagg ccgctcgaaa
     2701 atctgtaaat aatcacaccg tcaatctcgt ggatgtttgt gctctccctg atatcgcgga
     2761 aatgactgtg tcccggctgc acaccgagaa agcaggtggc cggctttgcg cttctgatta
     2821 tcatctcggc aaaagacaaa atgattccta tcaaaactcc gtttatcgta cccagaataa
     2881 gtactccgaa aaaagcacca acaaatatga aaaattctgt cctgctgacc ttccacagtc
     2941 tctttgccag atgaaactcc gtggcaccca tgagcgctga tatgacaatc gccgtgagca
     3001 ccggaaccgg cagatatcct ataaagcctg tgcaaaaaag aagcactgct atcatggaaa
     3061 cgcctgcaac aagccctgtg agctgtgtct taccctcgta ctgctcgctc atcgcagttc
     3121 gtgacactga accgtttatc ggacagcagc ccgtaaaagc cgcagccata tttcctatgg
     3181 aaaatgccaa aagctccgtg ttatcatcta ttctgtatcc gtttttctgt gcgaaattgt
     3241 tttctgccag aagtgtctct gccatgatga caacagcaac cgaaagactt aaaactatta
     3301 cctctgaggc cttttgaact gaaaaaactg cctcgaaatc cggtatatac caccgcggca
     3361 tgccgggctc aactgctgaa agagtcggca ctccccactc ctttaccggt ccaaaatatg
     3421 taaaaagcgc gcctgttacc atgagcacaa ccgccatagg aaactttgga acaagcctct
     3481 tactcacaac caaaatggca agcgctacaa tccccatgac aagtgcggct gcatttatgt
     3541 ggtgtaaggc ctcccatata tgctcagaca gttcaaaaag ctctccggtt cctgccgcag
     3601 agcccatgag ctttggcgcc tgcatcagta tgattgtagt acatatgcct gtgataaatc
     3661 cgcccataac cggcgcagat atgtagttca ccagctttcc ggcattcatg aagtaaaatg
     3721 ccaaaagcca cagtgccaca aaaaatgtaa acaccggtac aaccgtcatg gcctctttgc
     3781 ttcctgcctc tattcccatg ccaagcactg ctgctccgac aagcgccgcc ggagcagcat
     3841 ccacaccgaa tataaactgc ggtgatgtgg aaaacagagc aaacaatatg attgggaata
     3901 ccgagccata caagccatat accgccggta gcccggatat ctgcgcatag cccatcgata
     3961 taggaatcga aaccgccatg atgatgattc ccgcaagaat gtcctttggg agatttttct
     4021 tgtcgtagtt tttgagtgtt ggtgcaactg ataatttttg tttcatttag atttgtgcct
     4081 tctttatcaa tactttcttt ccttcaaatg cgaaaccata atgttgaatt gcttctcttt
     4141 tatatcctcg ttccaaaagc tttgcatcat aatttttctc ttcaatctgc ttcaaagcac
     4201 tatccgcagt catatccagt gatgtctcct cttgcgaatc aataacctta aattctatta
     4261 ttacagcctg cgaattttta tcacgtggaa tgatcatcac atcatatctg ccatagccac
     4321 tttcacggtt tgactgcact tcatatttat ctctaagctc gaccagcagg cctaagacaa
     4381 aaccatgata aaatctctcc ggttcagatt ttcccgaagg atgctttccg ctgtcaaagc
     4441 tgctgaaggt agataaagcg acctcattca tgtatatgtt catttccttt acattgcctc
     4501 taagcaatgc ccttatgaat ccattataat tcgaaacaga ggcattaaac catcctctga
     4561 acatatttga aaacatactt acggtttcaa gatttgtaat gctcagatga taccacggtt
     4621 caagcgtaag acctcttttt tctaaggaat caacctttaa ataaccgctg gcaagcaaaa
     4681 gactccaaat ggcattttca tcgttatcga gctgttcaaa caccagttgt tcgtcaatgc
     4741 taacctccac agaatcgcct tccagaagtg tttccatcat agattttatt tctgcagatg
     4801 ctgttctgat caaacgattt accagaccgt ttgagctggt agatgcccag tatggactta
     4861 tctgtttttc ctttagaaaa ttcgttatag accatggatt ataaatatca ctgtgatttc
     4921 caaatttaaa accgtcatac cattttttta cagtatcttt ttcaccggac agaccatact
     4981 cttccattgc atcaaaaact tcattctctg taaatccaaa gctcgttgca taactgcttg
     5041 aggtcgtggt tattacattc agattattga ggtctgaaaa aattgattcc tttgatacac
     5101 gcgtgattcc tgtcataata gcacgctcca tatacggatt tgtcttgaac gttgcattaa
     5161 acaggcttct tatatatgca gtaaactcat tccaatatcc atgaagatat gcctcctgta
     5221 taggagtatc atactcatca aggataatta tcgtcttctt tccccagtaa cgatgaaaat
     5281 actcacacaa aatatttaat tttaatccac tctcagcatc acccatcctg gtatttgttc
     5341 cttcaaaatc agctatttcg tctgctgtca gagtatcccc ctcaagcaga tatctgtgcc
     5401 ttttgtaaac agcctctatc tgtgctttta tctggatttt tgcatctccc agattgtcgg
     5461 ccttaaccga agcaaagctc ataaaaatca caggataagt accctgaatt tttcgatagt
     5521 cctcatcctc ccatatagac aatgaatcaa acaaatcgct cctgtcctta tactgatttg
     5581 aaaaaaagca gttaagcatg ctcatattaa gtgtttttcc aaaacgcctc ggacgcgtga
     5641 tgagagtcac atcatccttt gaatcccacc attcctttat aaagctcgtt ttgtccacaa
     5701 aaaagcaatt atcttctata acagaagcaa aatcctgttt cccaatacca atcgttttta
     5761 ccatatctga ctccctgtat agtctcaaat cattgtttta tatatgatat tatagccaat
     5821 acaattatgc actgcaagaa acaaatatac tcttcttaat cacgttttta aacatagatt
     5881 aagaagagta taatctcttt tgtcgtatta aattataact acttaattac agcctcaacc
     5941 tcacccttct caggcatcac gcgaagtgcg ccctttctcg tggtaataag cgaagctgct
     6001 gcatttgcga atgtgagcat ctttttcaca tcgtcatcct tccggtcatt gataccatgc
     6061 tccagcacat cgtgaagcag gcacgcacag aaggtatctc ctgcacctgt agtctcgaca
     6121 gtgtcatctc gcaggaacgg agctgcctcc acgatatgtc cgtcaaaaaa tgccatactt
     6181 ccgtttttgc ccatggtggc acagataaac ggaatgtgat actcatctat gagcttcttc
     6241 acacctgtct ttatgtcctt ttcgcctgtc atgaattcaa tctcatcatc agatattttt
     6301 aagatgtcac actgactcag gccccagctt atcttctctt ttgcatcgtc catgctcttc
     6361 cagagcggtt tgcgcagatt agggtcaaag gaaatcagta cgcctgcctc ttttgcggca
     6421 gctattgcat gctttgtcgc attctcacag atcttatctg tcattgaaag acttccgaag
     6481 tggaaaattt ttgcgtttct cacaagggat aaatccacct catccgctgt cagcatcata
     6541 tctgctcccg gatttctgta aaatgagaaa tctctgtctc cgtctgctgc ggtctggaca
     6601 aatgcaagag tggtatggac attatcatca tagcgcacgc ctgttgtgtc gatgccctgc
     6661 tcctttacgg catcaacaag cagccttccg aacgcatcct gtcccacttt tccgatgaaa
     6721 gcagtctgat gaccgagctt ttgaagcatg gcaagcacgt tacatggagc tccacccgga
     6781 ttagcctcaa aaagcttatt gtcctgcgca cttgtaccgt tttctgtaaa atctataagt
     6841 aattcaccta atgcaactac atccatattg tacctctttc tttcctgtca aaatatcctg
     6901 tatattattc tacaaataac cggaccaata atttgtccgg ttattatttg tatgctctac
     6961 tgatatgctt tcatattata aaattatatc atattatatc atatttttca atatccacaa
     7021 cagcacttcc gtcacaatta aagcagattc cgtctgcctg aagaggtgta cagatagctg
     7081 tggttgcaac ctgctgtccg tcattgataa agagctctat agactgtctg tccatgatga
     7141 aacgaagctt tagctttctg tctgtatcta caattttaat ttttctgaca cagacaacat
     7201 cctttgtgac gccacagcag gtacgatcaa tctcgagcac gcctgcgact ctgttgtagg
     7261 taaacactgt accatactca tcatcgcatg ccatttcaac agtgaagtta ttgtactcct
     7321 cgcctgaaag ctccactgtc atgtcgagag tgcggccctt tatgccatca aaggtcgtgt
     7381 agcagtctat ttttttatct gtataatgac acttgtttgc acgataattt tctatttcct
     7441 taaccggctt ctgccagatg cggccgtcct ttatttcaag ctcacgaggc agtgtcatca
     7501 tgccctgcca atcctgcgaa tcaggaacga cacatgcatc ccatgacttc atccacgcta
     7561 tcatcacgcg gcgtccgtcc ggaagcagtg tagtctgcgg tgcataaaag tccatgccat
     7621 agtcaagtgt gtgcggctct tccttgataa atacatgatt ttctttatca taatccccca
     7681 gaaaatatac agagttgtgt ccgttatgaa actcatattt gtctgccttc atactctgcg
     7741 gtgaacagat gagcacatgc ttatcctcca gcccaaagaa gtccgggcac tcccacatgg
     7801 tacctatttt gccggtgctg tttgccgcaa ggattgtctc aaactgccaa tcggtcaggt
     7861 tttttgaaga gcagagcact acctgtccca cattgtttaa gtccttgctt ccaactataa
     7921 gataatatgt gtcatcctct ttccatattt tagggtcacg gaaatcgatt ctgctgcagt
     7981 tttcaggaag catatcgcct gtcacaacag gattattctc atgcttcaca taattaagtc
     8041 cgtctccaaa tgcgatgcac tggttctgtc tctcctgctc actgccgtca ggaagtttaa
     8101 tacttgtgac acctgtgtat acaagcacat gcttaccatc tgcctcaatg gcacttcctg
     8161 agaaacatcc ttttttatca tattcctcgt caggtgccat cacacacgga agctgctccc
     8221 atttaatcat atcgcttgtc gtactatgcc cccaatgcat aggtccccac tcacgcagat
     8281 atggatagta ctgataaaac agatgaattt ttccgttata gacagaaaat ccattcggat
     8341 cattaatcca tcctgccggt gcactgatat gaaacaaagg tttactctct ttatctgtag
     8401 cagctatttt ttttgcctcg tagcttcttg ctttttccaa taattcgctc attcaaatcc
     8461 tccaattctg tttgctttgc cggtaataca gaattatgaa cagtaaccta tttgccctct
     8521 gacgtactct caagggattt ctgatagctg tcaaaatatt tctgctttat cttcagataa
     8581 tcctgtaaac catatttatc aagtttctta aggtatgaat cccattcctt atcaatgccg
     8641 ccgtttaata tccagtcggc ctttttctgc tcagcatatt tcttgatgtc tgtgtcgtac
     8701 tgtgacacct tgttggtgtc atctatgctc atgaacacgt ttggatagac atacttactg
     8761 ttcatgtcct tcagataagc ctcatgcatg ttgtcgagac gccacttggc atctgcaggc
     8821 tctgtcacat aaacatcata gtactcattt aaaacagcaa gcggtccgtt tacagactgc
     8881 gcctcacgca cctcaaccgg tgactgatcg cccagatcca tatgctttaa catctgtccg
     8941 ccgtcagcgt ttgtactcat ctcaaagatg ttaaacgaat ctttctcacc gtatgttccc
     9001 cagttattct gtggagactg gattggagca tacatctggt caatccacgc ggctgccaaa
     9061 gccgtatccc tgcagcttga tgtcagtacg catcgtcctc tgtcaaatcc actggtctca
     9121 ctgttgttct gtctggttat gtttctaact ccatccggtc ctgtgagtgc aggaagcatg
     9181 acataatctg tgttgttatc gatatttgcg atgtcccatg taaagcagag cccgtatctg
     9241 tggtttttac cctttgccac gtaggttgac cactcctgtg taaatgcctc agggtcaatc
     9301 aaatcctctg tgacaagctt gtgaagccac tcgataccct ccttgtagcc ctcctgtgtc
     9361 ggagtataga tgacttttcc ctcatctgtt actgcaaagt ggtcacctgt atcaccataa
     9421 ccctcaccaa agccgttcat gaggatggct ggatcctggt caccgttgtt tataataaat
     9481 gacataggaa tgacaccgcc ctcaatgtca aactctttct caagctcgtc tgcatgatct
     9541 ctgaatgcaa taagcacctg ctcgagctcg tctgtagtag tcggcacctc aagtcccaga
     9601 taatcaagcc atttcttgtt gatataaggt atatctccga ttgcctgaat tgcctctttt
     9661 cctgcaccaa gctgctctat ccaaggaaat gaatagatat gtccgtccgg tgcagtacac
     9721 atggttctgt actctggata tttttcaaaa actgcctgaa gattaggcat atattt
//
LOCUS       DPUF01000124            2508 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_20190, whole
            genome shotgun sequence.
ACCESSION   DPUF01000124 DPUF01000000
VERSION     DPUF01000124.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_20190"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="DHV81_04550"
     CDS             complement(<1..221)
                     /locus_tag="DHV81_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HCI93765.1"
                     /translation="MWCVDIYYKTISESGKQMINYVCKYAPVELFKGFGQECAVLEEM
                     PENFDKSDKIAHANLCGFGKSVIQAVLEG"
     gene            complement(243..1514)
                     /locus_tag="DHV81_04555"
     CDS             complement(243..1514)
                     /locus_tag="DHV81_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HCI93766.1"
                     /translation="MSIVDKFGEKVGEIAENDPVKARKLLLAGYRLQEKRLALFPDRK
                     LPMSGQYVAKIVMNNIIQALAKPENAAMVSIFVPGELLTAAGLTPYSVEALSCFMAGT
                     KCEQTFLKQTEKEGFPETMCSYHRVFLGAALNGIVPKPKCMVYTNLACDGNMMTFPYL
                     KERYEIPSFYIDVPYEKNYDSVMYVAKQLRELKNFLQDVTGRKITEESVKKAVDKSKI
                     ASNNYYRQLHLRKGHDIASTLTNELYALFMCHLLAGTDESVRYTQLLRDDVRRAPAGD
                     GFHVLWMHTMPFLQDAVKDVFNYSDTIHISASDFIADGFRSMESDDPYEALAEKMVYC
                     IYNGSVNQRIEEAKELADTVGADGAVLFAHWGCKNTIGASSLIKRSLEREGLPTMVLD
                     GDGCNPANASDGQISTRLQAFVEMLAEQERN"
     gene            complement(1613..2143)
                     /locus_tag="DHV81_04560"
     CDS             complement(1613..2143)
                     /locus_tag="DHV81_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93767.1"
                     /translation="MWYRKQEIIKMDKQIEIAGDDNKNNIGVLYGIGVGPGDPELMTL
                     KAINTIKACDIIAIPAVSKDECYVYGIVPSFCAAAARLGISLGEMMDEIHIIPASYDV
                     RDTVGYGGTCVYMKSGKKLAELIEVLRTGDAIRKKKMTVYGVTNCGMESERVYRGLDE
                     LTEAKGYLTIVIVKYS"
     gene            complement(2173..>2508)
                     /locus_tag="DHV81_04565"
     CDS             complement(2173..>2508)
                     /locus_tag="DHV81_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93768.1"
                     /translation="AETARLTNEWHEPDEDKKRELDEKMNELMMLYRKRNFNSDSLYD
                     ENVINTMTENYFEVTYFVPDEHKEGGSYEHVTGYLKKLDSIGHLMIVVDDNGRETVID
                     TRRIYDIDL"
BASE COUNT          660 a          659 c          387 g          802 t
ORIGIN      
        1 ttgccctcga gcacagcctg tatgactgat ttgccgaatc cacagagatt cgcatgagct
       61 atcttatctg atttatcgaa attctccggc atctcctcaa gcacagcaca ttcctgccca
      121 aagcccttaa acaactctac cggagcatat ttacaaacat aatttatcat ctgttttcca
      181 ctttctgaaa ttgttttata gtaaatatcc acacaccaca cacaataacc ggtgtattat
      241 ttctaattac gttcctgctc tgcaagcatc tccacaaatg cctgaagcct tgtggaaatc
      301 tgtccatcgc tggcatttgc cgggttgcag ccgtctccgt ctaaaaccat agttggaagc
      361 ccttccctct cgagtgagcg ctttataagg cttgatgcac cgattgtatt cttacagccc
      421 cagtgcgcaa aaagtacagc accgtccgct ccaactgtgt cggcaagctc ctttgcctcc
      481 tctattctct ggttcacgct gccattataa atgcagtaaa ccattttttc ggcaagtgcc
      541 tcgtatgggt catccgactc catgcttctg aaaccatccg ctataaaatc gcttgcactg
      601 atatggattg tatcactgta attgaacaca tccttcactg catcctgcaa aaacggcatg
      661 gtgtgcatcc aaagtacatg aaagccatct cctgccggtg cacgtctcac atcatctctc
      721 agaagctgtg tatatctgac tgattcatca gttcctgcaa gcagatgaca catgaaaagc
      781 gcatacagct catttgtgag agttgacgca atatcatgtc cttttctcag gtgaagctgc
      841 ctgtaataat tatttgatgc aattttgctc ttatctacag cctttttcac agactcctct
      901 gtgatttttc ttccggtgac atcctgtaaa aaattcttaa gctccctaag ttgctttgct
      961 acatacataa cagagtcata attcttctca tatggaacat ctatataaaa gcttggtatt
     1021 tcatatctct cctttagata cggaaatgtc atcatattgc catcgcatgc caggtttgta
     1081 tacaccatgc actttggctt tggcactata ccatttaatg ctgcgcccag aaaaaccctg
     1141 tgatacgagc acatagtttc cggaaagcct tccttctcag tctgcttgag aaaggtctgc
     1201 tcacatttag ttcctgccat aaaacacgat aatgcctcaa ccgaatacgg tgtgagacct
     1261 gccgccgtca aaagctcacc cggtacaaaa atactgacca tggcggcatt ttcaggcttt
     1321 gcaagtgcct gtatgatatt attcatgaca atctttgcca catactgccc tgacattgga
     1381 agctttctgt ccggaaataa tgctaatctt ttttcctgta atctatatcc tgcaagtaaa
     1441 agcttccttg ccttgacagg atcgttttct gctatctcgc ctactttttc tccaaattta
     1501 tctactatac tcatgtgata tttcagctct tttctatatt caaatatata tatataaata
     1561 ttcggttcac tatactatta tagcaattgt gcacaaaaag taaactccca ttttagctat
     1621 atttcactat aactattgtc agatatcctt ttgcttcggt aagctcatcc agccccctgt
     1681 acacacgctc agactccatg ccacagtttg tcacaccata cactgtcatt ttcttttttc
     1741 tgatagcatc tcctgtgcga agcacctcta taagctctgc aagcttcttt ccggatttca
     1801 tgtatacaca ggttccaccg tagcccacag tatccctcac atcatacgaa gccggtatga
     1861 tatgtatctc atccatcatc tcaccaagcg aaatgccaag ccgcgctgca gctgcacaaa
     1921 acgacggcac tatgccgtac acatagcatt catcttttga aactgcaggg attgcaatta
     1981 tatcacatgc ttttattgta ttaatagcct ttaaagtcat aagctcggga tctcccggac
     2041 ctacaccgat gccatataac acaccaatat tatttttatt gtcatccccg gctatttcta
     2101 tctgcttgtc catctttata atttcctgct ttctatacca catttacaag taatgcattc
     2161 ctaacaaaat catcacaaat cgatatcata tattcttctt gtatcaatta ccgtctctct
     2221 accattatca tccacaacta tcatcaggtg ccctatgcta tcaagctttt taagatatcc
     2281 tgtcacatgc tcatagctgc caccctcctt gtgctcatcc ggcacaaaat atgtcacctc
     2341 aaaataattc tctgtcattg tgtttatgac attttcgtca tataaactgt ctgaattgaa
     2401 attacgcttt ctataaagca tcatgagctc attcattttc tcatcaagct ctcttttttt
     2461 gtcctcatca ggctcatgcc actcatttgt cagcctcgct gtctctgc
//
LOCUS       DPUF01000125            2914 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_24070, whole
            genome shotgun sequence.
ACCESSION   DPUF01000125 DPUF01000000
VERSION     DPUF01000125.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_24070"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2438)
                     /locus_tag="DHV81_04570"
     CDS             complement(<1..2438)
                     /locus_tag="DHV81_04570"
                     /inference="COORDINATES: protein motif:HMM:PF01841.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93769.1"
                     /translation="MNKRLISFVLTFIMLITMVPSTIVEASEVTTMESVKVSSNQSES
                     NQSEVTKPEKNTIYAVNPLYEDVISIDDLKKKLDSSNDEQLFGASTGQYFSDYDSAVS
                     YLRKQMVSRETEINLNFPESWFTSHKDGLYWDLLYDAMKCDDSSTGQEGDALLYGYAG
                     CNVSYSTAGYIKYTMAYHSNAEQEAKLTAAVASAMTTLQLNGLSEAKKITKIHDYICN
                     HVDYEYNSKEEQIYTAYGALCTGKAVCQGYAVLFYRLCKEAGLSVRIISGTGNGGPHA
                     WNIVRIGSKYYNVDCTWDGQDAVTYNEFLLKSEADFRDHTRESWPVAGSHCLDYTSAE
                     FNAQYPMTEKSWDESTTYAHSEETTSGAVTLKAEWNDPVLGQPTIFHVSATGGSGNYK
                     FRMDAPSYSSPNQGAFESVADPSRGEWMNYTSECASNDYTFTMTATGTYNFRFYVMDK
                     ATNVYYLRLSFNIGVSDSKYPSVDSIVRSAVAECNSKTDGSEYAKALWLHDWLLDQLE
                     YDKTLKWSSAESALTRGLGTCQSYESAYAKLLTAAGIENSETRDTYDGHTWNAMKLDG
                     QWYQTDCTWDDSSDNWYSFDQRHLYFGLTDELMAIAHPGHSKIYTTDTYATRSTSLAD
                     NYFVRTGDAAKWAKAYSDRIQKNLDAGKTEFEITADNASYPPSISGIQNGITAYAINQ
                     MTWTTDKAAVTLNATGSAKSFTFTAEYTSESPAVSLYGRSITLKDNIDVNYYLEISDS
                     VFESDAYLEFKIGDQTYKLNVCDAAEVNENGKTLYKFSCPVNAAQMSDTIETRIVIDN
                     KTEEEYSYSVKEYAT"
BASE COUNT          752 a          688 c          518 g          956 t
ORIGIN      
        1 gtggcatatt cttttacact gtatgaatac tcttcctcgg tcttgttatc aatcactatt
       61 cttgtctcga tagtatcaga catctgtgcc gcattcaccg gacatgagaa cttgtataag
      121 gttttaccgt tctcatttac ctcagcagca tcacacacat taagcttata tgtctgatcg
      181 ccgattttga attccagata agcatcactc tcaaacactg agtctgatat ttccaagtaa
      241 tagtttacat ctatattatc cttaagggtt atacttctgc catacaagct gactgcagga
      301 ctctctgaag tgtattcggc tgtaaatgta aagctctttg cacttcctgt tgcatttaat
      361 gttaccgctg ctttgtctgt agtccatgtc atttgattta tcgcatacgc tgtaatacca
      421 ttttgaatac ctgaaatact tggtggataa gaagcattgt ccgctgttat ttcaaactct
      481 gtctttcctg catccagatt tttctgaatc ctgtctgagt atgcttttgc ccactttgcg
      541 gcatctcctg tccttacaaa ataattgtcg gcaaggcttg tcgaacgcgt tgcatacgtg
      601 tctgtggtat agattttgct atgccccgga tgtgctatgg ccatcagctc atccgtcagc
      661 ccaaaataca aatgtctctg gtcaaagcta taccagttat cgctgctgtc atcccaggtg
      721 cagtccgtct gataccactg tccgtccagc ttcatggcat tccaggtatg tccatcatag
      781 gtatcccttg tctcgctgtt ttctattcct gctgccgtaa gtagctttgc atatgcactc
      841 tcatacgact gacaggtgcc aagacccctg gtcagcgcac tctcagccga agaccatttc
      901 agggttttat catactcaag ctggtcaagc agccagtcat gcagccaaag ggcttttgca
      961 tactctgagc catccgtctt gctgttgcac tcagctactg cagaccggac aatcgagtca
     1021 acgctcggat atttactatc agatacgcct atattgaagc tcaaacgcag ataatataca
     1081 tttgttgctt tatccataac ataaaaccta aagttatagg taccggtagc agtcatggta
     1141 aatgtataat cgtttgaagc acactctgat gtataattca tccactcacc gcggcttgga
     1201 tccgctactg attcgaatgc cccttgattt gggctcgaat agctcggtgc atccattctg
     1261 aatttatagt ttcctgatcc gcctgttgca gacacatgga atatggtcgg ctgtcccagt
     1321 acaggatcat tccattctgc ttttagtgtc acagcacctg acgtggtctc ctcgctatgc
     1381 gcatatgtag tcgactcatc ccaggatttt tcagtcatag gatactgcgc gttaaactca
     1441 gcagacgtat aatccagaca atgactgcct gccaccggcc acgattcacg ggtatgatct
     1501 ctaaaatcag cttctgattt caacaggaac tcattatatg tgaccgcatc ctgtccatcc
     1561 caggtacaat ccacattata gtatttgctg ccgattcgca ctatattcca cgcatgtggt
     1621 cctccatttc cggtaccact gataattctg accgacagcc ccgcttcctt gcacaatctg
     1681 tagaaaagca cggcatatcc ctgacagaca gcttttccgg tgcaaagagc gccataagct
     1741 gtatatatct gttcttcttt tgaattgtat tcataatcga catgattgca gatataatca
     1801 tgaatctttg taatcttttt ggcttcgctc agaccattaa gctgcagtgt tgtcattgcc
     1861 gaagccacag ccgctgtcag ctttgcttcc tgctccgcat tagaatgata tgccatggta
     1921 tattttatat atcctgcagt actgtatgaa acattacagc ctgcatatcc atacaaaagt
     1981 gcatccccct cctgtccggt agagctatcg tcgcacttca ttgcatcata taataaatcc
     2041 caatatagcc cgtctttgtg tgaagtaaac caggattccg gaaaattcaa gttaatttcc
     2101 gtttctctgc taaccatctg ctttctgaga tatgaaacgg cactgtcata atccgaaaaa
     2161 tactgtcctg tggatgcccc aaagagctgc tcatcatttg atgagtcaag ttttttcttt
     2221 aaatcatcaa tgctgataac atcctcatac agtggattta ccgcatagat agtatttttc
     2281 tccggtttcg taacctctga ttgattgctc tcagattggt tactcgaaac tttgacgctt
     2341 tccattgtcg ttacttcact tgcctctaca atagttgatg gaaccattgt aatcaacata
     2401 atgaatgtta aaacaaagct aattagtctt ttgttcatac gttgttcctc ccgacttctg
     2461 ccattataaa acagcatcgc attgtttcag tttattgata acttatagtt ccatattact
     2521 attttattat gcatgttgca ataatttaat taacaattat tgggtttcct tagcctgtgt
     2581 atcatttctt tctatttcat tgattatttc atgtgatcat attatagatc tacttatcga
     2641 acatgatgca caacaaacga taagcttgtt aagccgataa gcacaaaaga aaccaccgca
     2701 ggaatttagc ttagttaaag cgttaaattc tgcggtggtt ttaattatgc agtcttttta
     2761 atttctatat ccataatacc aataccaata tagcgcgttc accagatttt ttaattcagt
     2821 agatgaattt tcgctattaa taaccttctg tgaatagctg aacggaccat agttcagctc
     2881 taaaatacag cccttattct tttcggtgac tttg
//
LOCUS       DPUF01000126            6396 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_30741, whole
            genome shotgun sequence.
ACCESSION   DPUF01000126 DPUF01000000
VERSION     DPUF01000126.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_30741"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="DHV81_04575"
     CDS             <1..796
                     /locus_tag="DHV81_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCI93770.1"
                     /translation="YSSFTKYATDPLVNITVMLLIIIGGIGFSIWNEVRTKKLKLSRY
                     SLHAKIVISTTLILVFGGALFMYIFEKNNTIAGMDTPGAIFASLFGSVTARTAGFNTV
                     DTAALTQESKLLTIVLMFIGGSPGSTAGGIKTTTMAVMIIYIVSYLRGSNGCNVFGRK
                     ISSEVIKKAGMVLIINLVLGLTAVIAILATSNMKMDDVLFEVYSAISTVGMTTGITRD
                     LNTVGRIIIIIMMYCGRIGSMTFVLSFVHRPDKANIELPEEKVIIG"
     gene            814..1464
                     /locus_tag="DHV81_04580"
     CDS             814..1464
                     /locus_tag="DHV81_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCI93771.1"
                     /translation="MKSILIIGMGKFGHHLCADLIKNGNDIMVMDKDEAKVRDLIYDV
                     TKVQIGDCTDPQVVKALGINNFDLIFVCIGTNFQSSLEITSLVKEMGGKYVISKANRD
                     VHAKFLLRNGADEVIYPDRDMAERVAKKYSANHVLDYVALNDDYSIYEINPPEGWVGK
                     SIKENNVRQKYNVNIVTVKEAGKNRVLPLPDYVFKADDAVMVLGLNADVDKLVRKL"
     gene            1528..2262
                     /locus_tag="DHV81_04585"
     CDS             1528..2262
                     /locus_tag="DHV81_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCI93772.1"
                     /translation="MGNNDSSVETFLQWIKASDNIVFFGGAGVSTESGIPDFRSVDGL
                     YNQKYDYPPETILSHTFYRKHPQEFYRFYRDKMLCLDAEPNITHYKLAELEKAGKLKG
                     VVTQNIDGLHQKAGSKNVMELHGSVLRNYCERCLQFVSAEEILHSTDVPKCPKCGGPV
                     KPDVVLYEEGLNQKTLEDAIYYISHADVLIVGGTSLAVYPAAGLIDYYNGNKLVLINK
                     STTPMDARADLLIQQGLGSVFEQIEV"
     gene            2320..2508
                     /locus_tag="DHV81_04590"
     CDS             2320..2508
                     /locus_tag="DHV81_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCI93773.1"
                     /translation="MDKYAVGDIVKLKKPHPCGSSEWEIMRVGADFRLKCMGCGHQVM
                     VPRKLVEKNDRGLTKKQA"
     gene            3012..3302
                     /gene="rpsF"
                     /locus_tag="DHV81_04595"
     CDS             3012..3302
                     /gene="rpsF"
                     /locus_tag="DHV81_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCI93774.1"
                     /translation="MNKYELALVVSAKIEDDARTAVVEKAKEYITRAGGTVTEVEEAG
                     KKRLAYEIQHMTEAFYYFIQFDAEGTVPAEIEQDVRIMDNVLRFLCVRKDEA"
     gene            3327..3770
                     /locus_tag="DHV81_04600"
     CDS             3327..3770
                     /locus_tag="DHV81_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCI93775.1"
                     /translation="MNKVILMGRLTRDAEIRYAQGDNSLAIARFSLAVDRRYSKNAEE
                     QSTDFINCVAFGKIAEFFERFGRKGTKFVVEGRIQTGSYTNKDGQKVYTTDVVVENAE
                     FAESKSAASGNAGGFAPADRPAPSQAAGDGFMNIPDGIDEELPFN"
     gene            3810..4067
                     /gene="rpsR"
                     /locus_tag="DHV81_04605"
     CDS             3810..4067
                     /gene="rpsR"
                     /locus_tag="DHV81_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCI93776.1"
                     /translation="MAYNKSADGSSMKRRPMRRRKKVCVFCGKDNVIDYKDTNKLKRY
                     ISERGKILPRRITGNCAKHQRALTVAIKRARHLALMPYVCE"
     gene            4222..4665
                     /locus_tag="DHV81_04610"
     CDS             4222..4665
                     /locus_tag="DHV81_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCI93777.1"
                     /translation="MDTYIVFWLAATVVFVLVELMTVGLTSIWFAAGSFVAFIIALLG
                     GNVVVQGIAFILVSVVLLALTKPWAGKYINSRTVKTNVDSLVGERAVLTEDADSMKQT
                     GKAVVNGQEWTVRPQDETQVIRKGELIEVVKISGVKLIVKRVKEA"
     gene            4672..5607
                     /locus_tag="DHV81_04615"
     CDS             4672..5607
                     /locus_tag="DHV81_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCI93778.1"
                     /translation="MEGLIALAVLLVIVLIVLVNCIKIVPQAHAMVIERLGGYLTTWS
                     VGLHLKVPFIDRIAKRVILKEQVVDFPPQPVITKDNVTMQIDTVVYFQITDPKLYAYG
                     VENPIMAIENLTATTLRNIIGDLELDETLTSRETINTKMRATLDVATDPWGIKVNRVE
                     LKNIIPPKAIQDAMEKQMKAERERREAILRAEGEKKSTILVAEGNKESVILDAEAEKQ
                     AAILRAEAKKEATIQEAAGQAEAILKIQQANADGLRMLKEANPDNAVLQIKSLEAFAK
                     AADGKATKIIIPSEIQGIAGLTKSIAEIAADKTEK"
     gene            5938..>6396
                     /locus_tag="DHV81_04620"
     CDS             5938..>6396
                     /locus_tag="DHV81_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCI93779.1"
                     /translation="MKKKIKFKGRLKSYMCWPLYLTFVWICADGIALRYDRRLGYGMV
                     GFTVFYFALVLFLYVRSKSVVANELISFATQYSSVQKRLLNEFEVPYALLEYSGKILW
                     VNDKFAELMGVDKKYHKSITTLFPTVTRELLTKSDAPTDFKIVNEEEDSIV"
BASE COUNT         2061 a         1251 c         1625 g         1459 t
ORIGIN      
        1 gtactcatcg tttacaaaat atgccacaga cccgttggta aatatcactg ttatgctgct
       61 tatcatcatt ggcggtatcg gtttttccat atggaatgag gtcagaacca aaaagcttaa
      121 gcttagcaga tattcgctcc acgctaagat agtaatatca accacactga tacttgtctt
      181 tggcggcgca ctgtttatgt atatttttga aaaaaataat accatagcag gcatggatac
      241 accgggtgcc atatttgcat cgttattcgg ctcggttacg gcgagaacgg caggcttcaa
      301 tacagtagac acagcagcac tgacacagga gagcaagctt ttgactatag tgctcatgtt
      361 catcggagga agcccgggtt cgacagccgg cggaatcaaa accacaacca tggcggtcat
      421 gataatctat attgtttcgt atctgagagg aagcaacggc tgcaatgtgt ttggcaggaa
      481 aataagcagt gaggtaatca aaaaggccgg aatggtgctt atcatcaatc tggtgcttgg
      541 acttacagcg gtgatagcta tacttgccac atccaatatg aagatggacg atgtactgtt
      601 tgaggtatac tctgcaatca gtacggtcgg catgacaaca ggcatcacaa gagatttaaa
      661 tacagtagga cgcataatta tcataattat gatgtattgt ggaagaatcg gaagcatgac
      721 ctttgtgctt tcgtttgtgc atagaccgga taaagccaat atagagcttc cggaggaaaa
      781 agttatcatt ggataaggat aaggagaaac gaaatgaaat caattttaat tattggaatg
      841 ggtaaattcg gacaccatct gtgtgcggat ttgataaaaa acggaaatga cattatggtg
      901 atggacaagg acgaggccaa ggtcagggat ttaatctatg acgtgaccaa ggtgcagata
      961 ggtgactgta ccgacccaca ggtagtaaaa gcactcggaa tcaacaattt cgatttgatt
     1021 tttgtatgca tcggcaccaa tttccagagc tcgcttgaga taacaagtct cgtgaaggag
     1081 atgggcggaa aatacgtcat cagcaaggca aacagggatg tgcatgccaa gttcctgctt
     1141 cgaaacggtg cggatgaggt tatctatcct gaccgcgaca tggcagagag agtggcaaag
     1201 aaatacagcg ctaaccatgt gcttgattat gtggcgttaa acgatgatta ctcaatctac
     1261 gagataaatc cacctgaagg ctgggtagga aagagcatca aggaaaataa tgtgcgccag
     1321 aaatacaatg taaatatcgt tacagtaaag gaagccggaa agaatcgcgt acttccgctg
     1381 ccggactacg tgttcaaggc agacgatgca gtgatggtgc ttggactgaa tgcagatgtg
     1441 gacaagctgg tgcgaaagct ataaaagtgt ggctcaaaaa ataataggca gcaatttaaa
     1501 gaagaaaaga agaaaggatg gatacatatg ggtaacaatg acagcagtgt agaaacattt
     1561 ttgcagtgga taaaggcttc ggacaatatt gtatttttcg gcggagcagg tgtatcaacc
     1621 gagagtggca tacctgattt cagaagtgtg gacggacttt ataatcagaa gtatgattat
     1681 ccgcccgaga ccatcctgag ccatacattt tacaggaagc atccgcagga gttttaccga
     1741 ttctaccgtg acaagatgct ctgccttgat gcagagccga acatcacgca ttacaagctg
     1801 gcagagcttg aaaaagccgg aaagcttaag ggtgttgtga cgcagaacat agatggactg
     1861 catcagaagg ccggcagcaa aaatgtaatg gagctgcatg gcagtgtgct gagaaactac
     1921 tgtgagaggt gcttgcagtt tgtaagcgca gaggaaatcc tccactcaac agacgtgcca
     1981 aaatgcccaa agtgcggcgg cccggtaaag ccggatgtcg tattgtacga ggagggctta
     2041 aatcagaaaa cgcttgagga tgcaatctac tacatcagtc acgccgatgt gctcatagtg
     2101 ggcggtacat cacttgcggt atatccggcg gcaggcctca tagactatta caatggaaat
     2161 aagctcgtgc ttatcaacaa gtccaccaca ccgatggacg caagagcaga ccttctgata
     2221 cagcagggac tgggctctgt atttgagcag atagaggtgt aaacagagga taatacacag
     2281 catattaaat ggcaggcata ggtagaagag gaatatagaa tggataaata cgcagtagga
     2341 gatatcgtaa aactgaaaaa accgcatccg tgcggcagca gcgagtggga aatcatgcgt
     2401 gtcggcgcag acttccgcct gaaatgcatg ggctgcggac accaggttat ggtgccaagg
     2461 aagctggtcg agaaaaatga ccgcggtctc accaagaagc aggcttagtt atatagaaac
     2521 aaagtagaat agtaaaaaca gaggtgcagt ttactgcacc tcgtcttttt atataaacta
     2581 aattcagcca cagagtaagg gacgtgcgtc gcgggcaggg ggagggcaca ggcatacttc
     2641 cgcaagagcg catagcgaag ctgcttagag gctgtagaat tgtgctacga agcacagaaa
     2701 tgccccggca tggaagccgg ggcaaggagc tatcgagcca tggatggcga gtagcgacgg
     2761 tttcgtcagg catagaaaac caaaatcaca attcgtacag ccacttagca gcgaagcgta
     2821 gctcacgcgg aagtatgcct gtgccctccc cctgtccgcg gcgcacgttc cgacctaata
     2881 aaaaaataaa gaaaaatgca aaaacccctt gaaaagtacc acgattcatg ttattatcta
     2941 gactcgtgat ataaaattcc ttgctcataa tcagatttat gagccagaga ccaaaaggag
     3001 gtaacattcg catgaacaaa tatgaattag cactcgtcgt tagtgcaaag atcgaggatg
     3061 atgcgagaac agcagtagtc gagaaggcaa aagagtacat cacacgtgcc ggcggtactg
     3121 ttacagaagt tgaggaggct ggtaagaaga gattagctta cgagattcag cacatgactg
     3181 aggcctttta ttacttcatc cagttcgatg ctgagggaac agtcccagca gaaatcgagc
     3241 aggacgttcg tatcatggac aatgttcttc gtttcttatg cgttagaaaa gacgaggcat
     3301 agtataagaa aataggagat tgtattatga acaaagtaat acttatgggt agattaacca
     3361 gagacgcaga aatccgctat gcccaggggg ataactcact tgcaatagca agattttcac
     3421 ttgcagtaga cagacgctac agcaagaatg cagaggagca gtcaacggat ttcatcaact
     3481 gcgtagcttt tggaaagata gcagaatttt ttgagagatt tggccgtaaa ggaacaaaat
     3541 ttgttgttga aggccgaatc cagaccggca gctatacaaa caaggacgga cagaaggtat
     3601 acaccactga cgtcgtggtt gagaatgcag agtttgcaga gtctaaatca gccgcatcag
     3661 gaaatgcagg cggatttgca ccggcagaca gacctgcacc tagccaggct gctggggatg
     3721 gcttcatgaa cattccggac ggaatcgacg aggagcttcc attcaattag tagtgtccac
     3781 gaggacaaat attcaaggag gtaacaaaaa tggcttacaa taaatcagct gatggttcat
     3841 caatgaagag aagaccaatg cgtagaagaa aaaaagtctg cgtattctgt ggaaaagaca
     3901 atgtcattga ttacaaagat acaaacaaat taaagagata catctctgag agaggaaaaa
     3961 tccttccacg ccgtatcaca ggaaactgtg caaagcacca gagagctctt acagtagcaa
     4021 tcaagagagc acgtcatctt gctcttatgc catacgtttg cgaataagat aaatgtatga
     4081 taaatcccga ctaccggatg gtggccggga ttttttaggt taaaaatgtt ttatgtttaa
     4141 aaaattaata tttgttgcat aatcaaatta catagtataa aatgaatata aaacaaatac
     4201 taataaaaag ggagggttca aatggataca tacattgttt tctggttggc agccaccgta
     4261 gtttttgttt tagttgagct tatgacagtc gggctcacat ccatatggtt tgcggcaggc
     4321 tcatttgtag cgtttataat cgcactgctt ggcggcaatg tcgtggttca gggaattgca
     4381 tttatactcg tttcagtcgt gcttttagct ctcacaaagc catgggccgg gaagtacatc
     4441 aattccagga cagtgaagac aaacgtggac agtctggtcg gagaaagagc tgtgctcacc
     4501 gaggatgctg acagcatgaa gcagacagga aaagccgttg taaacggtca ggagtggaca
     4561 gtcagaccgc aggacgagac ccaggtcata agaaaaggcg agcttatcga ggtagtaaag
     4621 ataagcggag taaaactaat tgtaaaaagg gtaaaggagg cttaaccgga aatggaagga
     4681 ttaatagcac ttgcagtgtt actcgtaatt gtacttatag tactggtaaa ctgcatcaaa
     4741 atcgtgccac aggcacatgc aatggtaatt gagcgtttgg gaggatatct cactacatgg
     4801 agcgtaggac ttcacctcaa ggtgccattt atcgacagaa tagcaaagag agtcatctta
     4861 aaggagcagg tggtagattt cccaccacag cctgtaatca caaaggataa tgtaacaatg
     4921 cagatagaca ctgtagtata tttccagatt acagatccaa agctctacgc atacggagta
     4981 gagaatccaa tcatggcaat tgagaacctc acagccacaa cacttcgtaa catcatcgga
     5041 gacttagagc ttgatgagac actcaccagc cgtgagacaa tcaacaccaa gatgcgcgca
     5101 acactcgatg tggcaacaga tccatggggc atcaaggtaa accgtgtcga gctcaagaac
     5161 atcatacctc caaaggccat ccaggatgcc atggagaagc agatgaaggc agagcgtgag
     5221 agacgagagg ccattcttcg tgcagagggt gagaaaaaat caacaatcct tgttgcagag
     5281 ggaaataaag agtcagtcat cctcgatgcc gaggctgaaa agcaggcagc catccttcgc
     5341 gcagaggcaa agaaggaggc aacaatccag gaggcagcag gtcaggcaga ggctatcctc
     5401 aagatacagc aggccaatgc agacggactt cgcatgttga aggaggcaaa tccggataac
     5461 gcagtgctcc agataaagag cttagaggca tttgccaagg cagcagacgg caaggctaca
     5521 aagataatca tcccttcaga gattcagggg attgcaggtc ttacaaagtc aattgcagag
     5581 atagctgcgg ataagactga gaagtaatta tatttaatca aaatgaatac cggaagatga
     5641 gagcacacaa aaagcatcgc atgtatcata tcaatatcag atatatgcgg tgctttttag
     5701 attgcacaga attgtgctta gattacacag gattgccctg aactcctgta attccaaact
     5761 gataaatata gtactaggaa taatcctcac aaagtggtat actctctagt aggtatatca
     5821 atcgatgcgg gctgcacaaa ggtgtggccg gtaactatta atttgtagta cattctcgaa
     5881 accgcaaccg ttgcaccggc agcatcagcg caacgcagcg gggataagga gagtaaaatg
     5941 aagaaaaaaa taaaattcaa gggcaggctg aaaagctaca tgtgctggcc gctatatctg
     6001 acgtttgtgt ggatatgtgc tgacggaata gcactgcgct atgacagaag gcttggctat
     6061 ggcatggtag gctttacagt gttttacttt gcgctcgtgc tgtttttgta tgtcagaagc
     6121 aagtcagtcg ttgcaaatga gctgataagc tttgcaacac agtattcatc ggtgcagaag
     6181 aggctgctta atgagtttga ggtgccgtat gccctgcttg agtactccgg caagatactc
     6241 tgggtaaatg acaaattcgc agagctaatg ggtgtggata aaaaatatca caaatccatc
     6301 acgacacttt ttccgacagt gaccagagag cttctcacca agagtgatgc gccgacagac
     6361 ttcaagatag taaatgagga ggaggacagc atagtc
//
LOCUS       DPUF01000127            3496 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6927, whole
            genome shotgun sequence.
ACCESSION   DPUF01000127 DPUF01000000
VERSION     DPUF01000127.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6927"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            1..2238
                     /gene="pflB"
                     /locus_tag="DHV81_04625"
                     /pseudo
     CDS             1..2238
                     /gene="pflB"
                     /locus_tag="DHV81_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515554.1"
                     /note="too many ambiguous residues; incomplete; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     assembly_gap    1515..1626
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2500..3281
                     /gene="pflA"
                     /locus_tag="DHV81_04630"
                     /pseudo
     CDS             2500..3281
                     /gene="pflA"
                     /locus_tag="DHV81_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569165.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
     assembly_gap    2727..2765
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1119 a          688 c          786 g          752 t
ORIGIN      
        1 ctttggaaag aggaaatcaa cgtccgtcag tttattcagg acaactacac tccatacgac
       61 ggagacgaga gctttttagc tgatccgaca gatgctacaa acaagctgtg ggatgcgctc
      121 cagaaattac agaaagagga gcgtgccaag ggcggagttc ttgacatgga gacagaggtt
      181 gtaacaggaa tcacagctta cggccctgga tatatagatg agtcattaaa ggacctcgag
      241 cagattgtag gtcttcagac agataagccg ttaaagagag cattcatgcc ttacggtgga
      301 atcaagatgg cggtacaggc tgctgagaca tatggttaca atgtaagcga caagctcaag
      361 gagattttca caaaatatca taagacacac aacacagcag tattcgatgc atacacacct
      421 gagatgatga aggttcgtca tgcaaagatc cttacaggtc ttcctgacac atacggccgc
      481 ggacgtatcg tgggcgatta ccgtcgtgta gcattatacg gacttgatta cctcatcgag
      541 gagaaaaaga aggataaggc aaactgtggc tgtggacaga tgacagatga tgttatccgt
      601 ttaagagagg aaatcgcaga gcagattaaa tgtcttgagg atatgaagaa gcttgcagag
      661 atttacggat acgatatctc aagacctgca acaaacgcaa aagaggcagt tcagtggtta
      721 tacttcggat accttgcagc aatcaagaca cagaatggcg cagcaatgtc agtaggacgt
      781 gtatctacat tccttgacat ttacatcaag agagatatgg acaagggcat cctcacagag
      841 caggaagcac aggagctcat cgaccatttt acaatgaagc ttcgtatggt taaatttgca
      901 cgtatccctt catacaacca gttattctca ggtgatcctg tatgggcaac acttgacgtg
      961 gcaggaacag gtgtggacgg acgttcaatg gttacaaaga cagacttccg tttcctccat
     1021 acattagaga acatgggacc tgcaccggag ccaaacctca cagtattcta ctcatcaaag
     1081 cttcctcaga ccttcaagga ttatgcagcc cgcatctcaa ttgagacaag ctcaatccag
     1141 tatgagaacg atgacgcaat gaagcctgta tggggcgatg attatgcaat ctgctgctgt
     1201 gtatctgcaa cacagacagg taaagagatg cagttcttcg gagcacgtgc aaaccttgca
     1261 aaatgtctcc tttacgctat caacggtggt gttgatgaaa aatcaggtga gcaggtagga
     1321 ccaaactacg cacctatcac agcagagtac cttgactatg acgaggtaat ggcgaaatac
     1381 gacaagatga tggactggtt agtagacatc tatgtaaata cattaaacct tatccagtac
     1441 atgcatgaca aatactacta tgaggcagca gagcttgcac tcatggatac agacttaaag
     1501 agaacatttg caacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnngcaa aggatttcga aatcgaggga gagttcccta aatacggaaa cgatgatgac
     1681 cgcgcggacg aaatcggtgt atggcttctt aagacattca tggacaagtt aaagaagcat
     1741 cacacatacc gtgattcaga gcctacaaca tcaatcctta caattacatc aaacgtagta
     1801 tacggaaaag ctacaggagc aatgcctgac ggacgtaaag ccggagagcc actctcacct
     1861 ggagcaaacc catcatacgg cgcagagcag aacggacttc tcgcatcctt aaactcactc
     1921 acaaagcttc catatgagtg ggcacttgat ggaatctcaa atacacagac catcaacccg
     1981 ggagcacttg gaaacaacga gggtgagaga attgacaacc ttgtaaacgt aatggacgga
     2041 tacttcgatc agggagcaca tcacctcaat gtaaatgtat tcggtaaaga gaagctcatc
     2101 gatgcaatgg agcatcctga gaaggaggag tacgcaaact tcacaatccg agtatcagga
     2161 tacgcagtta agttcatcga cctcacaaga gagcagcagc tcgatgtaat ctcacgtaca
     2221 tgccacgaga gaatgtaata taaaaaaata tccaacattc cagaatgtgc aggccaagtg
     2281 cctgcatatt ctgattttct aaataacacc acagcgtaag gaattttcgg aaggtgtgta
     2341 aaagccggtc gcatcaagca tagaaaactt aaatcataat tcatacagcc tcttagcccg
     2401 aagaacctga gcattgaaaa gtcccggtcg gcgggttgcc gaaaattccg taccataaaa
     2461 ataaaagtag cagcaacaac aacctaaagg aggtaaaaca tgggagaaac actaggaaga
     2521 gtacactcgg tagaaagctt tggctcagcc gacggcccgg gagtgagata tatagtattt
     2581 ttgaagggct gcaatatgcg ctgtaaatac tgccacaacc cggatacatg ggccaaatgc
     2641 ggcgaaaatg acggtgcaaa gctgatgaca ccgcaggagg tgctaaagac cgccatgcgc
     2701 tataaagcgt actggaagca aacaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnacagt aagcggaggt gaagcacttt tgcagataga ctttgtcaca gagctattta
     2821 agcttgcaaa ggaaaaaggt gtaaacacat gccttgacac atcaggaaat ccgtttaccg
     2881 tggaagaacc gtttttcggc aaatttaatg agctgatgaa atatacagat ctcttcatgc
     2941 tggacatcaa gcatatagat gacgaggagc acaaaaagct tacgggacag acaaataaga
     3001 atatccttga tatggcacaa tatctctcaa aaaacggtaa aaaaatgtgg atacgtcatg
     3061 tgcttgtgcc tggaatcacc acggatgaga gatatttaaa gcagcttaga gagtttatcg
     3121 acacacttaa aacagtggat agggttgagg tgctgccgta ccacacactt ggggtgttca
     3181 agtggaagga gcttggtata ccataccagc ttgagggggt ggagcctccg acagaggaac
     3241 agatagaatg tgcaaaaaga atattaaatt gtcagaatta gtttttaaat accattgcta
     3301 acaactcatt aagttgataa aatataataa gtaactatta ttttacaccg atgctgcctt
     3361 cgtttgtatg tgaggtgaca cttggctata tgcttgcgta tcgcaactgt aaatgtgctg
     3421 aacagttaaa atttaaaata acaatttgcg ggagttacaa catgggaaaa ccagatatca
     3481 tttacgaaga caacga
//
LOCUS       DPUF01000128            4771 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_893, whole
            genome shotgun sequence.
ACCESSION   DPUF01000128 DPUF01000000
VERSION     DPUF01000128.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_893"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="DHV81_04635"
     CDS             complement(<1..582)
                     /locus_tag="DHV81_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCI93780.1"
                     /translation="MKYISTLREGERINEIFLCKSKQTAMTKSGKPYDNVILQDKTGT
                     LDAKIWDVGSVGIEEFDAMDYIAITGDITSFQGNLQCSIKRARKVNEGEYDPADYLPV
                     SDKDVEVMYKELMGFIGSVKNQYLSQLLHSFFDNKEFERRFKFHSAAKSVHHGFVGGL
                     LEHTLGVTKNCDYFAKMYPVLNRDLLLTAAIFHD"
     gene            complement(601..1869)
                     /locus_tag="DHV81_04640"
     CDS             complement(601..1869)
                     /locus_tag="DHV81_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCI93781.1"
                     /translation="MEQFIREKIKDKPLNKKRLAKKAGTAALCGVAFAVAASIVFAIF
                     LPVISRQSKKASDGKNNNDVQTATQQSGIDDSSDAYSENGTQSTEPGTSSESSSQTYQ
                     PTLADYQAVQSQLYRVGALATKFVVGVTGVTDATDIFNNSYETEGQGVGVILKDNGKQ
                     LIILTEKDVVDKADKLSVTFVNDMMADAALVKYDSNTGIAIISVDKSLLDDATTAAIA
                     VAELGNSNIVSRGASVIALEANYAILTGLITSTTNELSAQDNNYSVLTTDIASNKLQS
                     GILINTDGQVIGLSLQDFNPAEENNTLTAVSISDLSPVIEKLESGADVPYIGITCTTV
                     TEKIANRYNIPKGVYIKQVTMDSPAFVSGLQSGDVIVAVNNTEVSNVSAYNTQLMKQK
                     PEDTCNLKVKRKGSNGYTEITCQVKIGVMN"
     gene            2421..4771
                     /locus_tag="DHV81_04645"
                     /pseudo
     CDS             2421..4771
                     /locus_tag="DHV81_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568377.1"
                     /note="too many ambiguous residues; incomplete; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     assembly_gap    4394..4421
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1292 a         1109 c         1055 g         1287 t
ORIGIN      
        1 atcatggaaa atagctgctg taagaagtag gtcacggttt aaaaccggat acatttttgc
       61 aaaataatca cagttcttcg tcacaccgag tgtatgctcc aaaagacctc ctacaaagcc
      121 gtggtgcacg ctctttgcgg ctgaatggaa cttgaaacga cgctcaaact ccttattgtc
      181 aaaaaagcta tgtaatagct gtgacaggta ctgattcttt acagagccga taaagcccat
      241 gagttcctta tacatgacct cgacatcctt atcgctgacc ggcagataat ccgcagggtc
      301 atattctccc tcattcacct ttctcgcacg ctttatggaa cactgcaaat tgccctgaaa
      361 gcttgtgata tcaccggtta tggctatgta atccattgca tcaaattcct caatacctac
      421 tgagcccaca tcccatatct tggcatccag cgtgcctgtc ttatcctgca ggataacatt
      481 gtcataaggc tttccgcttt tggtcatggc tgtctgcttg ctcttgcata aaaaaatctc
      541 attgattctc tctccctcgc ggagagttga aatatacttc atatatcctc ctaatttcgg
      601 ctaattcatc actccgattt tcacctgaca ggtaatctcc gtgtagccgt tgcttccttt
      661 tctctttact ttcagattgc aggtatcctc aggcttttgc ttcataagct gtgtgttgta
      721 cgcggacaca ttcgacacct ctgtattgtt taccgcaact atcacatctc cgctttgaag
      781 ccccgagaca aatgccggtg aatccatggt aacctgcttt atataaacac cctttgggat
      841 gttatatctg tttgctattt tttctgtaac tgtagtacag gtgatgccta tgtacggcac
      901 atctgcgccg ctctcgagct tttcaatgac aggtgacagg tcgcttatgc tgacagccgt
      961 gagcgtattg ttttcctccg ccggattgaa atcctgcagc gaaagcccta tgacctgacc
     1021 atctgtatta atcagtatac cgctctgcaa cttgttgctt gcgatatctg tggtgagcac
     1081 cgagtaattg ttgtcctgcg ccgacagctc attggttgtt gatgttataa gacctgtgag
     1141 aatggcatag tttgcttcaa gtgcaatgac agatgctcct cttgaaacaa tatttgaatt
     1201 accaagctcc gcaaccgcta tagccgctgt tgtggcatcg tccagaagtg acttatccac
     1261 ggaaatgatt gctatacctg tattgctgtc gtacttgacc agtgcagcat cagccatcat
     1321 gtcgtttaca aaggtcacac taagcttatc cgccttatcc actacatctt tctctgtcag
     1381 aatgattagc tgctttccat tgtccttcag tatcactccc acaccctgac cctcggtctc
     1441 ataggaattg ttgaaaatat cagtggcatc tgtcaccccc gtgactccta ctacaaattt
     1501 cgttgccaaa gctcccactc tgtatagctg gctctgcact gcctgataat ccgccagtgt
     1561 aggctggtat gtctgcgagg atgactccga agaagtgccg ggctctgtac tttgtgtacc
     1621 attttcacta tatgcatcac tgctgtcatc tatgcctgac tgctgtgtgg ctgtctgcac
     1681 atcattatta tttttcccat ctgaagcttt ttttgattgt ctgctgatta ccggcagaaa
     1741 tatagcaaaa acaatgcttg cagccactgc aaatgccact ccgcagagtg ctgctgtgcc
     1801 tgcctttttt gcaagtcttt ttttattcag cggtttatcc tttatctttt ctctgataaa
     1861 ttgctccata ttgttaccct gattagctgt ctttagttac tatcttacat tatattctga
     1921 attatattgc gttgcaacca tattttttct ttgagtgcgg gctttgagta cgggcttgcg
     1981 gcatgtaccc gccggactgc tgcactgctc ccaacggtgc gaatagcgca gtggcttaga
     2041 tactgtagga ttatgcttat aacacagaaa tgccacggca tggatgccgg ggcaaggagc
     2101 tatcgagcca tggaaggcga gtagcgacgg tttcgtcatg tatagaaaaa gtaactgcat
     2161 aatccataca gaatcttagt cacgcagcgt agcaccgttg tgagcagtgc agcagtccgg
     2221 cgggtacatg tcgcaagccc ttacttttta gctcatttaa caaactggtt tgttatgtat
     2281 taaactttat attatcatct tgtaattatc atttaataca tatttattat ggtttttttg
     2341 aagattttca gctataatat gataagctat acagtaaata tgaatttaat attcaactac
     2401 cgaaatataa ggatatagaa atgaataaaa aagtttacaa aacattggaa tataacaaaa
     2461 tcctcaccat gctaagcagc tacgcagcat gtgatgagac aaagaaaagg tgtctttcac
     2521 ttgagcctat caccgattta tacgaaatca gacatctgca gaccaccact gccgatgcac
     2581 taagccgcct gtataaggac agcggagtgt ccttcgttgg catacacaat gtacatgctt
     2641 ctctaaagag gcttgatatc ggtggtgcac tgaatacgac cgagcttctt cgcatctgca
     2701 gtcttttgga ggttgcaaag cgcgtgaagg cttatggcag aagcgccatg gataatgaga
     2761 agcaggattc tctctccggt ctgtttgcag ggattgaacc ggtttctgcg ctctgcgatg
     2821 agattaagag atgcatttta tctgaggaag agattgccga tgatgcgagt cctgagcttt
     2881 ttaagataag aaagagcata cgcggcatga acgacagaat ccatgcgcag ctcaccaagc
     2941 tcatgaacaa cagcacaaca aggacatatc tgcaggatgc ggttgtgacc acgcgcgacg
     3001 gcagatattg tctgcctgta aaggctgagg caaagggcaa tgtacctggc atgatgcacg
     3061 accagtcatc taccggctct acccttttta tcgagccgat ggctgtcgtc aatttgaata
     3121 atgagctcaa ggagcttttc ataaaggagc aggaggagat tgaaaaaatc ctcgcagcgc
     3181 tcagcgacaa agttgccatg aatgcggctg ctttggagca ggactatgag attctttcag
     3241 agcttgactt catttttgca aaggcaaatc tggctaaatc atataacggt gtggcacctg
     3301 agttcaacac cgaggggcat atcaatatca gaaagggccg tcatccgctt ttggatgcta
     3361 aaaaggtggt ccctatagat gtacggctcg gcgaggaata taagcagctt atcatcaccg
     3421 gaccaaacac cggcggtaaa acagtttcac taaagactgt cggacttttg actctcatgg
     3481 gtcaggcagg tcttcacatt cctgcggctg acaggtcaaa gctcgccatc tttgaggacg
     3541 tctttgcgga tataggcgat gagcagagta ttgagcaaag tctcagtacc ttctcgtctc
     3601 acatgaccaa tatcgtaaaa attcttgaaa aagctgacga cagaagcctt tgcctgttcg
     3661 atgagctctg ctcgggaacc gacccgacag agggtgcggc acttgccatc tcaattttaa
     3721 acagactgca ccggtatggc gccatcacca tggccaccac acactacagt gagctgaagg
     3781 tatatgcgct ttccacagac ggtgtggaaa atgcctgctg tgagttcaac gtggagactt
     3841 taagccctac ctaccgcctg ttaatcggta ttcctggaaa gagtaacgca tttgcgatct
     3901 catcaaagct tggtctggac gaaaacatca tcgaggatgc aaaaagcagg attaatgata
     3961 acgaccttga ttttgaggat ctgattgcaa gcctcgagtc acagcgacaa accatcgaaa
     4021 aggaacagct tgaaatcaac agctacaagg ccgagattga aaagctcaag aaacagctcg
     4081 aagataaaaa cgagcgcata gacaagagca aagacaagat tttacgcgag gcaaatgagg
     4141 aagcctacaa gatactgcag gatgcaaagg aacttgccga caaaaccatc cgcaatttca
     4201 acaaatacgg acaggggcag gctcctatga gccagatgga aaaggagcgc tctgcccttc
     4261 gtgacaagat gaatgacaag gaaaagaagc tgtcagacat caagaaaaac actgccaagg
     4321 caaatcacaa ggcgcctaag aagctgcgca tcggtgattc tgtgcttgtg ctctccctca
     4381 atctgaaggg aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nggaactgtc cacactctgc
     4441 caaacgccaa gggcgatttg tatgtgcaga tgggtatact gcgctcgctg gttaatatta
     4501 atgacctcgt gctcttaaac gacgaagtct ctcctgccaa gaagtacggc ggcagcggaa
     4561 gcaagataaa gatgagcaaa tcgctgtcag tttcatcaga aatcaacctg ataggaaaga
     4621 ctacggatga ggcgcttgct ctcttggata aatacttaga cgacgcatat atcgctcatc
     4681 tttcatcagt gcgcattgtc cacggaaaag gcaccggtgc actgcgaaag gctgtgcatg
     4741 gacttttgaa gaggacaaaa actattgcgg a
//
LOCUS       DPUF01000129            3649 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_11933, whole
            genome shotgun sequence.
ACCESSION   DPUF01000129 DPUF01000000
VERSION     DPUF01000129.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_11933"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="murA"
                     /locus_tag="DHV81_04650"
     CDS             complement(<1..1067)
                     /gene="murA"
                     /locus_tag="DHV81_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCI93782.1"
                     /translation="MEQYVIKGGNPLYGEVEIGGAKNAALAILAAAIMTDETVTIDNL
                     PNVRDINVLLQAIEEIGAHVERVDIHKVKINGSFIRGVNVDNEFIRRIRASYYLIGAL
                     LGKYKHAEVALPGGCDIGSRPIDLHMKGFRSMGADIDIAHGLVIARAKELKGTHIYMD
                     KVSVGATINIMMAAAMADGKTVIENAAKEPHVVDVANFLNSMGANIRGAGTDVIRIVG
                     VEKLHATEYSVIPDQIEAGTFMFAVAATGGNVLVKDVIPKHLEATTAKLLEVGCQVEE
                     FDDSVRVISDGHLKHTQVTTLPYPGFPTDMQPQMAVLLGIAEGTSTVTESIFENRFKY
                     VDELTRMGADIKVESNIAIISG"
     gene            complement(1255..1761)
                     /locus_tag="DHV81_04655"
     CDS             complement(1255..1761)
                     /locus_tag="DHV81_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCI93783.1"
                     /translation="MEQKTNIVLIGMPGVGKSTIGVILAKVLGYSFLDADLLIQEQEG
                     KLLREIIEEKGTDGFIEVENRVNASIRADRAIIATGGSVVYGKEAMEHLKEIGRVVYL
                     KVSYAILEKRLADIKGRGVVLKKGQTLETLFEERSKLYEQYADIEVSEEGLDVEQTVE
                     KLVEALEL"
     gene            complement(1745..2997)
                     /locus_tag="DHV81_04660"
                     /pseudo
     CDS             complement(1745..2997)
                     /locus_tag="DHV81_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515548.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
     assembly_gap    2163..2218
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3161..>3649)
                     /locus_tag="DHV81_04665"
     CDS             complement(3161..>3649)
                     /locus_tag="DHV81_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCI93784.1"
                     /translation="EDISDKFDTTVDIVTDAYIPADYIANEFQKLDIYKRIAGIDTEE
                     EKDEMLEELIDRFGEPPKSVLSLLRVARLKALAHAVYITEIKQTGSLIKLTMFERARI
                     NPEKIPELIAQYKSSLKFNMAENPYFTFDLKNGSVAKKRDVLDVVEELISQMRKELIT
                     AE"
BASE COUNT          836 a          930 c          634 g         1193 t
ORIGIN      
        1 ccgctgatga ttgctatatt gctctctacc tttatatcag ctcccattct ggtgagctcg
       61 tcaacatact tgaaacgatt ctcaaatatg ctctctgtca ctgtactggt gccctcagcg
      121 atacccaaaa gcactgccat ctgtggctgc atatctgtcg ggaagcccgg atatggaagt
      181 gttgtgacct gtgtatgctt taaatgtccg tctgatatta ctctgactga gtcatcaaac
      241 tcctctacct ggcagcctac ctccaacagc tttgctgtgg tggcctcaag atgctttgga
      301 atgacatcct ttaccagcac attgcctcct gttgccgcaa ctgcaaacat gaatgtaccg
      361 gcctcaatct gatccggaat gacagaatac tctgtcgcat gtaatttctc cacacctaca
      421 attctgatga catcggttcc tgcgcccctg atatttgctc ccatgctgtt taagaagttt
      481 gccacatcaa cgacatgagg ctccttagcc gcattctcga taacagtctt gccatctgcc
      541 atggctgcag ccatcataat attgattgtg gcacctacag aaaccttatc catatatatg
      601 tgtgtgccct taagctcctt tgccctggcg atgacaaggc catgagctat gtctatgtct
      661 gctcccatgg agcggaagcc cttcatatga aggtcaatcg gcctgcttcc gatatcacag
      721 cctcccggaa gcgcaacctc tgcatgcttg tattttccaa gtaatgcacc aatcaaataa
      781 tatgatgccc tgattcgtct gatgaattca ttatcgacat ttacgcccct gataaatgaa
      841 ccgtttatct ttaccttgtg tatatccacg cgctccacat gtgcgccgat ttcttcgatt
      901 gcctgaagta agacattaat atcccttaca tttggcaaat tatctattgt tacagtctcg
      961 tctgtcatta tggcagcggc caaaatagca agtgcagcat ttttagctcc accaatttca
     1021 acttcaccgt ataaaggatt tccacctttg attacgtact gttccatatt ccacctctat
     1081 gaattcagga gtatacttcc tcctaatata ctattctcaa tttacactat tgttatacat
     1141 ccaactacaa atttcattta tgacgacgta tttaagccgc catttgtgcc ggtgctgctt
     1201 ttaataaatg acgcagcagc tactattatc taatcatatc acgataaata agcactataa
     1261 ttcaagtgcc tccaccagct tctctactgt ctgctccaca tcaagcccct cctcggaaac
     1321 ctcgatgtct gcatactgct catacaattt tgagcgctct tcaaaaaggg tctctaaagt
     1381 ctgccctttc tttaatacta cacctctacc ctttatatct gcaagtcgtt tttccaaaat
     1441 agcataagaa accttgagat acacaactct gcctatctcc ttaagatgct ccatggcctc
     1501 ttttccgtat accaccgaac cgcctgtagc tataatggcc ctatcagccc tgatagaggc
     1561 attgacacgg ttctctacct ctataaaacc atccgtgccc ttttcctcaa ttatctcacg
     1621 cagaagcttt ccctcctgct cctgtatcag taaatccgca tccaggaagc tgtatcccag
     1681 cacctttgca agtattacgc ctatggtact ctttccaact cccggcatgc ctataagtac
     1741 gatattagtt ttctgttcca tctactgcct cagtattctc tgtatctgta ctattggtgc
     1801 tttctgactg ctgtgtgctc tctgtagcgc ttgtgtctgc tgcctttttg tccttcttgg
     1861 tagtagtaaa ataattgtga atcttgatct tgtcaagctg cttctgcttt acagaccatt
     1921 tttcatcgtc ctgccatcct gaaaggatgc cattgtaata tttgtcctct ttattgccct
     1981 ttacggtttt tctgtttgac tctgttgctt cctcatcagt ctttttgtca agccttacga
     2041 tatagtaagt actgtctgta gtgatgaggt ctgaggtctg tccctcctcg agggaattga
     2101 gtgcatctac aacgctctga tcaagattgt ttgtagtgta tacactgtcc tttgtcttgc
     2161 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     2221 ctctgtgtca tctgatttct tatctgaatc atctgtcttc tccgcatccg gatcaacgta
     2281 tgtggcgtat gtgcctgttg tggctgtagt gcctgctgcc ttggctgcat cctctaagga
     2341 tgatccgcct gcaagcgcct ctaccactgc atctgcctgt gcctttatct gtgctgactg
     2401 ctcatctgta tatgtctgat actggtaatt agagtcatat gcacccttgt aatcaagctt
     2461 cacaatggtg tatgcagcca tattggcttc ctcatccgtc acgcttgaat cagcattttt
     2521 gatgattgca gcatacatct tactctttat gaggcgaagc tctaaatatt cctcgacaat
     2581 ctcctctgta gcactcatct cgtctatcac atcatcggaa tttgccttca taaacttctc
     2641 tgcggccttt ttgatttcct ttttctcatc atcagaaacc tctacatcgt aatctgactg
     2701 atgcgccttt aatgtgtaaa gctcatgagc ctcctcgata gcatttttct tccatgtttc
     2761 aagcacagta ttattgcctg atactgtctt gtcccagtag ccctcgccca tgtactgtat
     2821 atacatgtca tcataaccgg cctcctggta ccttatcata aaattaacaa gcccaagctt
     2881 gatatcctct ttatctaatg tcgcaactgt tgcggtttta tttattgaac atcctgtaaa
     2941 agctgtaact gagagtgttg cagctaataa catagctatt acttttttcg ctttcattgt
     3001 atctatcctc tttaaaattg gtatagtatc gctcgtatat tatagagctt ttcgggcctg
     3061 ctggcaagca ttttctaaac tttgtaaatt atcgtcaatt tcgcgtaaag catgtggtgt
     3121 ttcctatgct atatgctaca tgattccaaa attcgggtaa ctattcagcc gtaatcagtt
     3181 ccttacgcat ctgcgaaatc agttcctcca ccacatcaag cacatcccgc ttctttgcca
     3241 cgctgccatt ttttaaatca aatgtgaaat atgggttttc tgccatattg aatttgagtg
     3301 aggatttata ctgggcaata agctccggta tcttctccgg atttatcctt gcccgctcaa
     3361 acatggtaag ctttatcagt gagccggtct gctttatctc agtgatatat acggcatgtg
     3421 ccagcgcctt aagccttgcc acgcgaagca ggctgagcac tgattttggt ggttctccaa
     3481 acctgtctat gagctcctcg agcatctcat ccttctcttc ctctgtatct atgcctgcaa
     3541 tacgcttgta tatatccaac ttctggaatt cgtttgcgat ataatctgcc ggaatataag
     3601 catcggttac gatatccacg gtcgtgtcaa atttatcact gatatcctc
//
LOCUS       DPUF01000130           17139 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_5232, whole
            genome shotgun sequence.
ACCESSION   DPUF01000130 DPUF01000000
VERSION     DPUF01000130.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 17139)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17139)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17139
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_5232"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="DHV81_04670"
     CDS             <1..201
                     /locus_tag="DHV81_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCI93785.1"
                     /translation="VNVGKAKVYVDGEYHFTADPHINNWQHCNAAIIFNNKTSENHDV
                     RIEIAEEDRDKQFTILGFGYVL"
     gene            221..>1787
                     /locus_tag="DHV81_04675"
     CDS             221..>1787
                     /locus_tag="DHV81_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HCI93786.1"
                     /translation="MVTAKNPILSGFYPDPSICRVGEDYYLVTSSFVYAPGVPIFHSR
                     DLAHWEQIGNILDRPSQLCVENEEISRGIFAPTIRYHEGTFYMITTNVSHGGNFIVTA
                     QDPAGPWSDPYYLGEEAVGIDPSLFFDDDGRCYYCGTRPNPEGVRYNGDWEIWIQELD
                     LGTMKLKGRSMAIWKGAVKGCIWPEGPHIYKINGYYYLMHAEGGTGPEHSITIARSKE
                     LFKWFEGCPRNPIFTHRNLGMDYPVIYAGHGDLVDDINGNWYVVMLASRPCKKHSSMG
                     RETFIAKTIWENEWPVIAPGIGHLEDTVDIPLEECRFIDEISENDFITFCEAKPDKRL
                     VGIGKRNESFYSLKENPGVLRLYTNKEQIADLGTSAFLGLRQKGYEFSVKTAVRFIPQ
                     SDNETAGLVLFQNNENHLRAEITMEAGRLVFVVTTHIKGTDKKIAVADIMEYSITEKN
                     PLWNICMICKEQEASIWIGTAEKSVKVAEKISLLPYTTEEAGGFVGCTVGMYASSNGS
                     NSDNYAEFSYIMLT"
     assembly_gap    1788..1934
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1935..2641)
                     /locus_tag="DHV81_04680"
     CDS             complement(<1935..2641)
                     /locus_tag="DHV81_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI93787.1"
                     /translation="MIENLNGIHETVKYKENSNVKLFINVEVEDYPIHWHTPMEIIMP
                     LEGSYTVQIGDDLVHLNESDIIFIASGVKHRLIAPDTGKRLILQIEWSAVSKIKELNS
                     ILTLIAPAITLTPETSGDIHSNIRKLLLDIADEYQTDSFLSEAVIYSKMLEVLVLIGR
                     HHTASSCHFNVGRHKQQEYLERFLSICEYIDTHCTEDITLDEIAKIAGFSKYHFTRLF
                     KQFTNNTFYKYLNQKRI"
     gene            2800..4944
                     /locus_tag="DHV81_04685"
     CDS             2800..4944
                     /locus_tag="DHV81_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCI93788.1"
                     /translation="MSKKTMEYAKKLVSQMTIDEKISQMLYESPAIERLGIPEYNWWN
                     EALHGVARAGVATVFPQAIGLAATFDTDLIEKIGDVVSTEGRGKFNEFSKKGDHGIYK
                     GLTFWAPNVNIFRDPRWGRGHETYGEDPYLTGKLGCAYIRGLQGDDPDHLKSAACAKH
                     FAVHSGPEAIRHEFDAKASKHDMYDTYLYAFKRCVKDAKVEAVMGAYNRVNGEPACGS
                     RTLLKDILRDEFGFEGHVVSDCWAILDFHEHHHVTDTVEESAAMAVNNGCDLNCGSAF
                     LHLKDAYDKGLVSDEAITAAVERLMEVRIRLGMMKDYPSPYEDISYEVVECKEHVELS
                     VEAARRSLVLLKNKDNFLPLDRKNVKTIAVIGPNANSRDALIGNYYGTSSRYITPLEG
                     LQQYLGEDTRVLYAEGCHLYKDKVQGLAEEKDRFKEALIMAEQSDVVVMCLGLDATIE
                     GEEGDAGNEYASGDKLGLMLPGLQEELLEAVAAVGKPVILVLSAGSAIDLSWAEEHVD
                     AIIDSWYPGARGGKAVAEAIFGEYSPSGKLPVTFYQGTENLPEFTDYSMAHRTYRYTN
                     ENVLYPFGYGLHYGETNYDGLSVDKAESDVNEPVEVFVNVTNDSRYTVNEIVQLYIRH
                     VDAAEYEPGYQLKGIEVVKLEPHETKKVKLTLSPRDFAVIEEDGSCVAVPGIYEISAG
                     GQQPDDRSTKLTGKRTERIEIARCGEKTGVDY"
     gene            5040..6806
                     /locus_tag="DHV81_04690"
     CDS             5040..6806
                     /locus_tag="DHV81_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein"
                     /protein_id="HCI93789.1"
                     /translation="MRKSIWNAIERKLDDYSLRKKFYIFYVLCVMLPLIVTDAVVLSI
                     VNNAEKESSRHEMSSIASAVGYNLSSMVNNAGEAAKSIYTSKRIDDFISKEYTSSAEY
                     VSEYQSFFQDTLFENTLGMSNIVFFLYADNPTIVNGGKVNDMSAVRNTDSYRLLKQKG
                     SGGLFFVYEKGGAGLSDERRMIYMQNLDFYSSDIEKVLKVEFNYGSMMRTLKNMNYDN
                     EVLICHGDDIVLSNGAYSGVNKPFKTLEEIGKISDSVYRQQISLYGCDLDIYVFKTRS
                     NIWSMLMHNAPIIVFLMLINVFFPLCLVSIFNHSFTSRILKLSKVFKSVDSEKLVSMT
                     GRESKDEIGSMIKNYNRMVSRTNDLIQTVYKNKLKEQEMLVSQKNAELLALHSQINPH
                     FLFNALESIRMHSLLKRENETADMVEKLAIMQRQYVEWGNDAVTVAKECEFVKAYLAL
                     QKYRFGERLNYNIDIEADCMECQVPKLTLVTFVENACVHGIESKAAPGWIFVRIFKKA
                     GFMHIEVEDTGSGIDPDALEKMKSNMKNADIDMLKTGGRVGVINACLRIKMVTESKAV
                     FDVDSEEGCGTTVMIKIPETYL"
     gene            6817..8412
                     /locus_tag="DHV81_04695"
     CDS             6817..8412
                     /locus_tag="DHV81_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HCI93790.1"
                     /translation="MLKVLLVDDEPFIIQGLKVIIDWENEGFEIAGMVSNGKEALQFL
                     ENENVDLVIADIRMPEMTGLELLETLRKDKKSDVYFVILSGYADFSYAQQAMQNDCTD
                     YILKPVDKEMLLKVLNKVLVLKNEKNKIDEDNKKMKQAYLARHLISLIRGKYDEVNLN
                     YVKENMRCEGGARYVEIQVEQLPAGDEILDSDMRSLQRKMYESCVAFLGADSAHCVFD
                     VSVNEKVYDIGFIFSDYMEEEAAKNERKYLEDLRRYICDNTQKNIVMLVGRKVSDISK
                     IARSYGNACMLRSFQGFRIVKSIYYYEDEVKISADGIVLCKDSLDELLRTVEQNNHVE
                     IRNAVATFYDDMSSMGMNGESMNLNINYLLFQFIHLASEQDDNVNQQEILRLISESSF
                     KTGIERGSRAHMTRFCCEYGDYLSQLRKNVSRGVIGMVEKEVKDNYATNITLKSLSEK
                     YYVNSAYLGQLFRKKYGQSFKDYLNNYRMERAAELLLRTDKKIIQIAEEVGYHDMDYF
                     VNRFIQVKGCTPARFRRQMQSGE"
     gene            8552..10216
                     /locus_tag="DHV81_04700"
     CDS             8552..10216
                     /locus_tag="DHV81_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCI93791.1"
                     /translation="MRRKKIVSMLLVATMAATLFAGCGNDSGKKGSSGGAKEFDAFFA
                     VPGSEINDDNEIQKIIEEKTGVRVKETWLTGQTADEAIGTMIAGGEYPDFIEGASGQK
                     QLYEAGALVPLDDYIEKYPNIKNLFTELEWEKLRQDDGHIYWIPQFSCIKGDEKVCTH
                     NDEAFWIQARVLKWANYPQIKTLDQYFDLIEKYNEANPTMADGTENIPYTILCEDWRY
                     FCLENAPQFLDGYPNDGSCMVDPDTKKVLDYNTSDTAVKYFKKLNEEYNKGIVDPESF
                     TQTYDEYISKLSTGRVLGMIDQWWDFAYTAGDAIKQAGLAEQGCDYIPVPVTIDGRDN
                     QWHNAGGAFNEATGLAVTTACDDVDAAMKFVNDLLDQDIHNLRFWGVKGTDYEVDENG
                     EFYRTADERKQASDTAYKASHLCSYSYFPQYNGTSDDGINANKPDGQAREFYDGLNSD
                     VQEAFDAYGVKTYVEMLGTNDAPGDWYPMWSYSNNFNTSTPGGVAWTKIGEVKHEQLP
                     QVVMAKNFDKAWDTYMDAYNACNPQDFLDELQTELDKRLEQAAKFK"
     gene            10290..11246
                     /locus_tag="DHV81_04705"
     CDS             10290..11246
                     /locus_tag="DHV81_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lplB"
                     /protein_id="HCI93792.1"
                     /translation="MSLTRENKKEKVRKPRTKITWKEVKRQKVLLFWAAIMVAYGVVF
                     YYLPLAGWAMAFQDYKPRLGIFHSALVGLDKFKMLFSDMTFIRVIRNTLAMGVINLVV
                     TFVTAIAFAILLNEIASKGGKKVVQTISYLPHFLSWIIVTGILHDMLSGTGIVNEFLV
                     NMHIIDQPINFFAHTGYFWPIVAFANVWKETGWNAIIYLAAITSIDPSLYEAASIDGA
                     GRWAKIKYVTLPGIKPTIIILLLMNVGNVLNAGFEIQYLLGNGLIQKVSQTIDIYVLK
                     WGISQGDYAIGTAAGIFKSVVSIILIFIANKIAKHNGEEQLF"
     gene            11261..12169
                     /locus_tag="DHV81_04710"
     CDS             11261..12169
                     /locus_tag="DHV81_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI93793.1"
                     /translation="MADITAGSVKSKKKKKISIFPIVNCIIMVLFLIITLYPVLNTLA
                     ISLNDGTDALRGGIYLLPRKFTWKNYITVLQKDNLITGAYITVARTIIGTVLALVANA
                     ILAFIVSRKRFLFKRGLSLFWVITMYVNGGLIPTFLLYKGLGLTNSFWVYVVPGMVSA
                     FNMLVIRTYMNGIPDSLEESAQLDGAGYTTIFLKIYSPLCKPVYATVALFVAVGQWNS
                     WFDAMLYNRMNTKLTTLQYELMKLLSAVTNQGGSAETLKNAVGAVTPTSVRAAATIIT
                     MLPIICIYPFLQKYFVTGLTLGGVKE"
     gene            12367..13866
                     /locus_tag="DHV81_04715"
     CDS             12367..13866
                     /locus_tag="DHV81_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCI93794.1"
                     /translation="MNSQQYNRANKRVFLAVIIVFAYIAFTLFAAVGTKDADITRISI
                     QLVAAIAVIVIAVIAFITKRDSRTGALILVSAMTAGYFIIALINSTIGTWTYALPLVI
                     AAMIYLDIKMMMVMNAVIIISSVIRLVMQLGIGGTVLQNDVIAVFVLVLVGYASDSIT
                     ILLTHFFDENMEEIKESAMAQVDSNKKMVMVAENISKHFDEAVTMLNDLDESVAVSHS
                     SIQEIADSTESTAEAIQKQAAMCVDIQGNTDNAESGIKAMIDASRQTDETVKQGADVV
                     EELKEQAHNVAEASNVTVSVIQSLTAKVEEVKSFVESIINISNQTNLLALNASIEAAR
                     AGEAGKGFAVVADEIRQLSEQTKDASANITEIINKLNEDTRRANESIENSVSSVEKQN
                     ELIDDTRDKFNAMGEAVELLMKDINVAEESIKKILDSTTVISDNITHLSATGEEVAAS
                     STEGLRMADTTVESMAKCKKVLENIYMLADDLKNSVEESEEELSQKFDF"
     gene            14025..14903
                     /locus_tag="DHV81_04720"
     CDS             14025..14903
                     /locus_tag="DHV81_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93795.1"
                     /translation="MDDNNYMNNDNRSSGTPDNTGVFGQSSETANGSDTYSQPQDTYN
                     QPTDTYNQSVSQQESGNAYGQSSDQTGSTDGYVNNPGSAYGYANQGTYNQNPNAQGAY
                     NQNPNAQGTYNQNTYAQGSFNQSAYGQTQYGQQPNGQGGYNQTAYGQTAYNQPQYGAN
                     PYNQPPKNSNGFGIASLVLGIASIVLFCTCINYVTALLAIIFGIIHIIKGNNKVFGIV
                     GIATAAISVIVSIIFWVVAGSNASTDFEKEFMKGFDEGYEYYNDDDSYEDYDYDYDSF
                     DYDDFDYQNFEYDGIY"
     gene            15309..>15583
                     /locus_tag="DHV81_04725"
     CDS             15309..>15583
                     /locus_tag="DHV81_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucyl-tRNA synthetase"
                     /protein_id="HCI93796.1"
                     /translation="MYNKVDTNMNFVEREKKTEQFWRENNIFRKSMENRKEGETYTFY
                     DGPPTANGKPHIGHVETRTIKDMIPRFQTMKGKYVPRKAGWDTHGLPV"
     assembly_gap    15584..15730
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15731..>17139
                     /locus_tag="DHV81_04730"
     CDS             <15731..>17139
                     /locus_tag="DHV81_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCI93797.1"
                     /translation="FWADMDNPYVTYDDNFIESEWWALKEIWNKGLLYKGFKIVPYCP
                     RCGTPLSAQEVAQGYKTVKEKSAVVRFKVVGEDAYFLAWTTTPWTLPSNVALCVNPDD
                     TYVKVKAVDGYTYYMAEALADKVLSQLLSKEDAEAGKKAYEVLETYKGKDLEYKEYEP
                     LYDCAKQVADKQGKKGFFVTCDTYVTMSDGTGIVHIAPAFGEDDANVGRNYDLPFVQF
                     VNDKGELTAETPFAGMWVKDADPEVLKDLSGRKQLFDAPKFEHEYPHCWRCDKPLIYY
                     ARESWYIKETAVKDDLIRNNNTVNWIPESIGSGRFGNWLENIQDWAISRNRYWGTPLN
                     IWECECGHRECIGSRAELAEKAGDPKAAEVELHRPYIDAVTIKCPECGKDMHRVPEVL
                     DCWFDSGAMPFAQHHYPFENKEVFEKQFPAKFISEAVDQTRGWFHSLMAESTLLFNKA
                     PYENVIVLGHVQDENGQKMS"
BASE COUNT         5443 a         2876 c         4124 g         4402 t
ORIGIN      
        1 gtaaacgtgg gaaaggcgaa ggtatatgtg gacggagaat atcattttac ggctgatcca
       61 catatcaata actggcagca ctgcaatgcg gcaataatat ttaacaataa gaccagtgaa
      121 aatcatgatg taagaattga aattgcagag gaagacagag acaagcagtt tacgatactg
      181 ggctttggat atgtattata ggaaatgaga ggtaagagac atggtaacag caaaaaatcc
      241 aatactttca ggtttttatc ctgatccatc aatatgcaga gtcggggaag actattatct
      301 ggtgacatca agctttgtgt atgcaccggg tgttcccata tttcatagcc gggatctggc
      361 tcattgggag cagattggca atatacttga cagaccatcg cagctatgcg tggaaaatga
      421 agaaatatcg agaggtattt ttgcaccgac cataagatat catgaaggaa ctttttatat
      481 gataacgacc aacgtatcgc atggcggcaa ttttatagtg acagcgcagg atccggcagg
      541 accgtggtct gatccgtatt atttgggaga agaagcagtc ggaatagacc caagcctttt
      601 ctttgatgat gacggcagat gttattactg tggcacacgc ccaaaccctg aaggagtacg
      661 ttacaatgga gactgggaga tatggataca ggagcttgat cttggaacca tgaagcttaa
      721 gggcaggagc atggccatat ggaaaggtgc tgtaaagggc tgcatatggc cggaaggacc
      781 acatatctac aagataaacg gatattatta tctgatgcat gcagagggcg gcactggacc
      841 ggagcacagt ataacgatag caagaagcaa ggagcttttt aaatggtttg aaggatgtcc
      901 gagaaatccg atatttacac acagaaatct gggaatggat tatccggtca tctatgcagg
      961 acatggtgat ctggtggatg atataaacgg aaactggtat gtggtcatgc ttgcgtcaag
     1021 accatgcaaa aagcacagca gcatgggcag ggagaccttt attgcaaaaa caatatggga
     1081 aaacgaatgg ccggtaatag cacccgggat aggacatctt gaggatacgg tagatatacc
     1141 gcttgaagag tgcagattta tagatgagat atcagaaaat gattttataa ctttctgcga
     1201 agccaagcct gataagaggc tggttggaat aggaaaacgc aacgagtctt tttactccct
     1261 taaggaaaat ccgggagtac tgagactgta cacaaacaaa gagcagatcg ctgatcttgg
     1321 cacaagcgca tttcttggac taagacagaa gggctatgag ttttcggtaa agacagcagt
     1381 gcgttttatc ccacagtcgg ataatgagac ggcaggactt gtacttttcc agaacaatga
     1441 gaatcatctt cgcgctgaga taacaatgga agccggaagg ctggtatttg tggtgactac
     1501 acatattaag ggaactgaca aaaagatagc agtggcagat attatggagt acagcattac
     1561 agaaaaaaat ccactgtgga atatatgtat gatctgtaag gaacaggaag catctatatg
     1621 gataggtacg gccgaaaaga gcgtaaaagt cgcagaaaaa ataagtctgc tgccatatac
     1681 cacagaagaa gcaggtggct ttgtgggctg tacggtcggt atgtatgcat cctcaaatgg
     1741 cagcaatagt gataactatg cagaattttc atacataatg ctcacccnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnntctatt cttttctgat ttaagtattt gtaaaaggta ttattggtaa
     1981 actgcttaaa tagccgggta aagtgatatt tactgaatcc ggctattttt gcgatttcat
     2041 caagtgttat gtcctcagtg caatgtgtat cgatatactc acagatagac agaaaacgct
     2101 ccagatattc ctgctgcttg tgtctgccga cattaaaatg acaactgctg gcagtatgat
     2161 gtcttccaat aaggacaagt acttcaagca ttttggagta gatcacggct tctgatagaa
     2221 atgagtcggt ctggtattca tctgctatat caagcagcag tttacggata ttgctgtgga
     2281 tatcaccgga agtttccggg gttagcgtta tagcaggtgc tataagggta agtatggaat
     2341 ttaattcctt tatttttgag acagcagacc actcaatctg caggataagc ctttttccgg
     2401 tgtcaggtgc gataagcctg tgttttacac cggaagctat aaagataata tcactctcat
     2461 taagatgtac caaatcatcc ccaatctgca ctgtatagct gccttcaagc ggcatgataa
     2521 tctccatggg agtatgccag tgaatgggat aatcttctac ttcaacattt ataaaaagct
     2581 ttacattgct gttttcttta tattttactg tctcatgtat tccattcaga ttttcgatca
     2641 tatatacttg tctccatacg gctgatattt atgcgcattt gcgtgaatat agttattata
     2701 gcacaaatag caatttttga acattgtggg gcaacaatta gaaagaaaat actgcatgaa
     2761 tctggtaaaa tcattatata aattaaagga gaagtcacta tgagtaaaaa aactatggaa
     2821 tatgcaaaaa aacttgtatc gcagatgaca atagatgaga agataagtca gatgctgtat
     2881 gagtcaccgg ctatagaaag actggggata ccggaataca actggtggaa tgaggctctt
     2941 catggagtag caagagccgg tgttgctaca gtatttcctc aggcgatagg tcttgcggca
     3001 acttttgata ctgacctgat agaaaagata ggagatgttg tatcaacaga gggtcgtgga
     3061 aagttcaatg aattttcaaa aaagggtgat cacggaatat acaagggact taccttctgg
     3121 gcacctaatg taaatatatt cagagatcca agatggggca ggggacatga gacatacggg
     3181 gaagatccgt acctgacagg taagcttggc tgcgcatata taaggggatt gcagggagat
     3241 gatccggatc acttaaaatc ggcagcctgt gcaaagcatt ttgcggtaca cagcggtccg
     3301 gaagccataa gacatgagtt tgatgcgaaa gcttcaaagc atgatatgta tgatacatat
     3361 ttgtatgcct tcaagcgatg tgtaaaggat gcaaaggttg aggctgtcat gggggcttac
     3421 aacagggtaa atggagaacc tgcctgcgga agcaggacac tgcttaagga tatactgcgt
     3481 gacgagtttg gatttgaagg tcatgtggtg tcagactgct gggcaatact tgatttccac
     3541 gaacaccatc atgtgacgga tacagttgag gaatcggctg ctatggctgt aaacaacggc
     3601 tgtgacttaa actgtggaag tgcattttta catcttaagg atgcttatga caagggactg
     3661 gtttcggacg aggcgatcac ggcagcagta gaaaggctga tggaggtgcg tatccgtctg
     3721 ggcatgatga aggattatcc atcgccatat gaggacatat cgtatgaggt tgtagaatgt
     3781 aaggaacatg tggagctttc tgtggaggct gccagaagaa gtcttgtact tttaaagaat
     3841 aaggacaatt tcctgcctct cgacagaaaa aatgttaaaa ctattgcggt tatcggacca
     3901 aatgccaact ccagggatgc gcttatagga aattattatg gtacctcatc aagatatatc
     3961 actccacttg agggattgca gcaatatctt ggagaggata caagagtgct ttatgcagag
     4021 ggctgccatc tgtacaagga caaagtacag ggacttgcag aggaaaagga cagatttaag
     4081 gaagcactca tcatggcaga acagtcagat gtggtcgtaa tgtgtcttgg acttgatgca
     4141 actatagaag gtgaagaggg tgatgccgga aatgaatatg caagcggtga caagctgggg
     4201 cttatgcttc cgggacttca ggaggaactt ttagaggctg ttgcagcagt aggaaagccg
     4261 gtaatacttg tgctgtcagc aggaagtgcg atagatcttt cctgggcaga agagcatgtg
     4321 gatgccatta tagacagctg gtatccggga gccagaggcg gaaaggctgt tgcagaagct
     4381 atattcggag agtatagtcc gagtggaaaa ctgccggtta cattttatca gggcaccgaa
     4441 aatctcccgg agtttacgga ttattcaatg gctcacagga cataccgcta tacaaatgag
     4501 aatgtgttat atccatttgg atatggactg cattatggtg agacaaacta tgatggactg
     4561 tcagtggata aagcagaaag tgatgtaaac gaacctgtcg aggtgtttgt aaatgtgaca
     4621 aatgacagca gatatactgt aaatgagata gttcagctct atatcaggca cgttgatgct
     4681 gcagagtatg agccgggata tcagctaaag gggattgagg ttgtaaagct tgagccacat
     4741 gagactaaga aagtaaagct gactctttcg ccaagggact ttgctgtgat agaagaggat
     4801 ggaagctgtg ttgctgtgcc gggaatttat gagatatccg caggaggaca gcagcctgat
     4861 gacagaagca caaagctcac aggcaagagg acagagcgca ttgagatcgc aagatgtgga
     4921 gagaaaacag gggtagatta ttaataataa atatgttatt ttataattaa tgacaagtaa
     4981 aacagacttt aggagcgggc atatgtcggc tcctttaagt gatttcagag gaactgttta
     5041 tgagaaaatc aatatggaat gccattgaga gaaagcttga tgattattca ctcagaaaaa
     5101 aattttacat attttatgta ctgtgtgtca tgctgccgct catagtgact gatgcagtgg
     5161 tgctttccat cgtaaataat gctgaaaaag aaagcagcag acatgaaatg tcgagcatag
     5221 caagtgctgt gggatataat ctgagcagca tggtcaataa cgcaggagag gcagctaaga
     5281 gtatctatac cagcaagcgc atagatgatt ttatatcaaa ggaatacaca agctctgctg
     5341 agtatgtatc agagtatcag agcttctttc aggatacact ttttgaaaac acacttggaa
     5401 tgagcaatat agtgtttttc ctgtatgctg acaatcctac aatagtaaat ggcggaaaag
     5461 taaatgatat gtctgctgta agaaatacgg attcttacag acttctgaag cagaagggaa
     5521 gcggaggact cttttttgta tatgaaaaag gcggagccgg attgtcagat gaaagacgta
     5581 tgatttatat gcaaaatctg gatttttact catctgatat cgaaaaggta ctcaaggtag
     5641 agtttaatta tggcagcatg atgcgtactc ttaagaatat gaattatgat aatgaagtgc
     5701 ttatctgcca tggagatgac atagtgctgt caaatggggc ctacagtgga gtcaacaaac
     5761 catttaagac attggaggag attgggaaaa taagcgactc cgtatacagg cagcagatct
     5821 ctttgtatgg ctgtgatctt gatatatatg tgtttaagac acgcagtaat atatggagca
     5881 tgcttatgca caatgcaccg atcattgttt ttctgatgct tataaatgta ttctttccac
     5941 tttgtctggt tagtatcttt aaccattcct ttacgagcag gatattaaag ctttcaaagg
     6001 tatttaagtc agttgacagc gaaaagcttg tatctatgac tggcagggag agcaaagatg
     6061 agatcggcag catgataaaa aattacaaca ggatggtaag tcgcacaaat gacctgattc
     6121 agactgtata taagaataag ctaaaagagc aggagatgct tgtaagtcag aaaaatgcag
     6181 agcttcttgc actgcacagc cagataaatc cgcactttct gtttaatgcg cttgagagca
     6241 tacgaatgca cagcctgctg aaaagggaaa atgagacggc tgatatggtt gaaaaactcg
     6301 caatcatgca aagacagtat gtggagtggg gaaatgatgc tgtaaccgtt gcaaaggaat
     6361 gtgaatttgt aaaagcctat ctggcattgc agaaataccg gtttggcgag cggctgaact
     6421 ataacataga tatagaagca gactgcatgg aatgtcaggt gccaaaactt acgcttgtaa
     6481 cctttgttga aaatgcctgt gtgcatggca tagaaagtaa agctgctccg ggctggatat
     6541 ttgtgcgtat attcaaaaaa gccgggttca tgcatataga ggttgaggat acaggcagcg
     6601 gaattgatcc ggatgctctt gaaaaaatga aaagtaacat gaaaaatgca gatatcgata
     6661 tgttaaagac cggtggcaga gtgggcgtga taaatgcctg cctgcgtatc aaaatggtaa
     6721 cggaatctaa agcagtattt gatgtggaca gtgaggaagg ctgcggtacg actgtgatga
     6781 taaaaatacc ggaaacatat ttataggaga tttaaaatgc ttaaagtatt attggtggat
     6841 gatgagcctt ttataataca ggggcttaag gtgatcattg attgggaaaa tgaaggattt
     6901 gagatagcag gaatggtctc aaatggaaag gaagccctgc agtttcttga aaatgagaat
     6961 gtggatctgg tcatagccga tataaggatg ccggagatga caggacttga gctgcttgag
     7021 actttgagaa aagataaaaa atcagatgtg tattttgtga tattaagcgg ttatgcagat
     7081 tttagctatg cgcagcaggc tatgcagaat gactgtacgg attacatact aaagccggtg
     7141 gacaaggaga tgctgcttaa ggtactaaac aaggttcttg tgctcaaaaa cgagaaaaac
     7201 aagatcgacg aggataacaa aaagatgaaa caggcatacc ttgcaagaca cctgatatct
     7261 ctgatacggg gcaaatatga tgaagtaaac ctgaactatg taaaagaaaa tatgaggtgc
     7321 gagggtggag caaggtatgt tgagatacag gtggagcagc ttccggcagg tgatgagata
     7381 ctcgatagtg atatgagaag cttacagcgc aagatgtacg aaagctgtgt tgcttttctg
     7441 ggggctgatt cggcacattg tgtatttgat gtatcggtaa atgaaaaagt ttatgacata
     7501 ggctttatat tctcggatta tatggaagag gaagccgcaa aaaacgagag aaaatatctg
     7561 gaggatctga gaaggtatat atgtgataat acacaaaaga atatagttat gctggtcgga
     7621 agaaaagtat cagatatatc aaagatagcc agatcatacg gcaatgcatg tatgctcagg
     7681 tcgtttcagg gctttcgcat agtaaagagc atatattact atgaggatga agtaaagata
     7741 tcggcagatg gaatagtgct ttgtaaagat agtctggatg agctgcttag gacagtggaa
     7801 cagaacaacc atgttgagat aagaaatgca gttgccacat tttatgatga tatgtccagc
     7861 atgggtatga atggggaaag catgaactta aatattaatt atctgttgtt tcagttcata
     7921 cacctggcaa gtgagcagga tgataatgta aatcagcagg agattttgcg actcataagt
     7981 gagagcagct ttaagaccgg tattgagagg ggaagccgtg cacatatgac cagattttgc
     8041 tgtgagtatg gtgattatct gtcgcagctt agaaaaaatg tgtcaagagg tgtgatcggc
     8101 atggtagaaa aggaagtaaa agacaattat gccacgaaca tcacgcttaa gagcctgagt
     8161 gagaagtatt atgtaaacag tgcatactta gggcagcttt tcagaaaaaa atacggacag
     8221 tcctttaagg attatctgaa caattacagg atggaacggg cggcagagct tctgcttagg
     8281 actgataaga agataataca gatagcagag gaagtcggat atcatgatat ggattatttt
     8341 gtgaacaggt ttatacaggt aaaaggctgc acaccggcgc gtttcagacg gcagatgcaa
     8401 agtggagaat aatatatatt actgcaaaac agggtaaatt atagtttttt cgagaaacta
     8461 tagtttaccc tgtttttgac tatagttact ccgattgttt gaaaacttaa aaagtataaa
     8521 attaagttat caaaagggag gacattacaa tatgagaaga aaaaaaattg taagcatgct
     8581 gcttgtggct actatggcag caacactgtt tgccggatgt ggtaatgata gtggcaaaaa
     8641 aggtagctct ggaggtgcga aagagtttga tgcattcttt gctgtaccgg gatctgaaat
     8701 caatgatgat aatgaaatcc agaagatcat cgaggaaaag acaggtgtaa gggtaaagga
     8761 aacctggctt accggacaga cagcagatga ggcaatcgga acaatgatcg caggtggcga
     8821 gtatcctgat ttcattgaag gtgcaagtgg acagaagcag ttatatgaag caggtgcact
     8881 tgttccattg gatgactaca tcgagaaata tccaaacatc aaaaatcttt tcacagaact
     8941 tgagtgggaa aaacttcgtc aggatgacgg acatatctac tggataccac agtttagctg
     9001 cataaaggga gacgagaaag tctgcacaca caatgacgag gcattctgga tccaggcaag
     9061 agtacttaaa tgggctaact atccgcagat caagacactg gatcagtatt ttgacctcat
     9121 cgagaaatat aatgaagcaa atccaacaat ggctgatgga acagaaaata ttccatatac
     9181 aatcctttgt gaagattgga gatatttctg tcttgagaat gcaccacagt tccttgacgg
     9241 atatccaaac gatggatcat gtatggtaga tcctgataca aagaaagtac ttgactataa
     9301 tacatctgat acagcagtta aatatttcaa gaagctcaat gaagagtata ataagggaat
     9361 tgtagatcct gagtcattca cacagacata tgatgaatat atctctaagc tttctacagg
     9421 acgtgtactt ggaatgattg accagtggtg ggattttgca tacacagcag gcgatgcaat
     9481 taaacaggca ggtcttgctg agcagggatg cgattacatt ccggtacctg taacaatcga
     9541 cggccgtgac aatcagtggc acaatgcagg cggtgctttc aacgaggcaa caggtcttgc
     9601 tgttacaaca gcctgtgatg atgtagatgc ggccatgaag tttgtaaatg atcttcttga
     9661 tcaggatatc cacaatctca gattctgggg tgtcaagggt actgactatg aagtagatga
     9721 gaatggtgag ttctacagaa cagcagatga gagaaagcag gcttctgata cagcatacaa
     9781 ggcttctcat ttatgctcat actcatactt cccacagtac aatggaacaa gtgatgatgg
     9841 aatcaatgcc aacaaacctg atggacaggc aagagagttc tatgatggtc ttaacagtga
     9901 tgtacaagaa gctttcgatg cctatggtgt taagacatat gttgaaatgc ttggaacaaa
     9961 cgatgcacct ggagattggt atccgatgtg gtcatactct aacaacttta acacaagcac
    10021 tcctggtgga gtagcctgga caaagattgg cgaagttaag catgagcagc ttccacaggt
    10081 tgttatggca aaaaactttg acaaagcatg ggatacatac atggatgcat acaatgcatg
    10141 caatccacag gatttcctgg atgaattaca gactgagctt gataaaagat tagagcaggc
    10201 tgctaagttc aaataaagct taaatgaaat ttaaggcagg cagatgtaat agcgttttat
    10261 ctgcctgctg ccttttaagg agtggtgtca tgtcattaac gagagaaaat aaaaaagaaa
    10321 aggtcagaaa acctagaaca aaaattacat ggaaagaagt caaacgccag aaagtgctgc
    10381 tgttctgggc agcaataatg gtggcatatg gagtcgtatt ctattatctg ccgctcgctg
    10441 gctgggcaat ggcatttcag gattacaaac caagacttgg tatatttcat tctgcattgg
    10501 ttggattgga caaattcaag atgctgttct cggatatgac atttatccgt gttataagaa
    10561 atacactcgc aatgggcgtt atcaatctgg ttgtaacttt tgtcacagcc atagcatttg
    10621 caatcttact taatgagatt gcatcaaagg gtggaaagaa agtagtacag actatatcat
    10681 atcttccaca tttcctttca tggatcattg taacaggtat tttacacgat atgctttcag
    10741 gaacaggtat tgtaaatgag ttccttgtaa atatgcatat tatagaccaa ccgataaact
    10801 ttttcgcaca tacgggatat ttctggccga tagtggcatt tgccaatgta tggaaagaaa
    10861 caggatggaa tgcaatcata tatcttgcag ccattacatc aatagatcct tcactttacg
    10921 aagcagcatc tattgatgga gcgggacgct gggcaaagat caagtacgta acactgccgg
    10981 gaatcaaacc aactatcata atccttcttc ttatgaatgt aggtaatgtt ttaaatgcag
    11041 gatttgagat acagtatctc ttaggaaacg gtctgataca gaaagtatca cagactattg
    11101 atatttatgt acttaaatgg ggcataagcc agggagatta tgcaataggt actgcagcag
    11161 gtatattcaa gagtgtggta agtataattc ttatatttat tgcaaataaa attgccaaac
    11221 ataatggtga agagcagttg ttttaaggag gtcttgtacg atggcagata taacagcagg
    11281 ctctgtaaag agcaaaaaga aaaagaagat aagtatattt ccaattgtaa attgcatcat
    11341 tatggtattg tttttgatca taacgcttta tccggtgctt aatacacttg cgatatcgct
    11401 taatgatggt acagatgcat taagaggtgg aatttacctc ttaccaagaa agtttacctg
    11461 gaagaattat ataacggttt tacaaaaaga taatcttata acaggagcat acattactgt
    11521 tgccagaaca ataattggaa cagtcttagc actggtggca aatgcaatcc ttgcttttat
    11581 agtcagcaga aaaagatttt tgttcaaaag aggtttatcc ctgttttggg ttatcacaat
    11641 gtatgtcaat ggtggattaa tccctacctt cctcctgtac aagggacttg ggcttaccaa
    11701 tagtttttgg gtttatgtag tccctggaat ggtcagtgcg tttaacatgc tcgttatcag
    11761 gacctatatg aatggaattc ctgacagctt agaggaatca gcgcagcttg acggtgcagg
    11821 atataccacc atttttctga agatttattc acctctctgc aaaccggtat atgcgacagt
    11881 agctttattc gtagcagtag gacagtggaa ctcatggttt gatgcaatgc tttataaccg
    11941 tatgaataca aagcttacta cattacagta tgagcttatg aagcttcttt cagctgtaac
    12001 aaatcaggga ggatcagcag aaacattgaa aaatgctgta ggtgcagtta cacctacctc
    12061 agttcgtgcc gcagcaacaa ttataacaat gcttccgatc atatgtattt acccgttttt
    12121 acagaaatac tttgttacag gacttacact tggtggagtt aaagaataaa tattttttta
    12181 caggatgccc ggcaggataa ctgccgggtg ttttggtgta aatgtttatt tgaaaggaga
    12241 attttcttca aatcaggagc aaaatgagaa aaatggtacg ttggaactaa atagtagtgg
    12301 attattataa aaaactgttg tataataaaa ctactttaaa aactcaaaca aaagaagggg
    12361 gattttatga atagtcaaca gtataacaga gccaacaaac gtgtattcct ggcggtaatc
    12421 atagtatttg catatatagc atttacgtta tttgcagcag ttgggacaaa agatgctgat
    12481 attaccagga taagtataca gcttgttgcg gcaatagcag taatagtaat agctgtaatt
    12541 gcatttatta caaaaagaga cagcagaaca ggtgcgctta tacttgtaag cgccatgaca
    12601 gcaggatatt ttattatcgc actgatcaat tcaacaatcg gtacatggac atatgccctg
    12661 ccgcttgtta ttgcagcgat gatatatctg gatataaaga tgatgatggt gatgaacgca
    12721 gtaataatca tatccagcgt catcaggctt gtaatgcagc ttggaatagg gggtacagtt
    12781 cttcagaatg atgtgatagc agtttttgta cttgtacttg tgggatatgc gtcagacagc
    12841 atcacgattc ttcttaccca tttcttcgat gaaaatatgg aagaaatcaa agaatctgct
    12901 atggcacagg tggacagcaa taagaagatg gtaatggttg cggagaatat ttcaaagcac
    12961 tttgacgagg ctgtgaccat gttaaatgat ctcgatgagt ctgttgcggt gagccatagt
    13021 tcaatccagg agatagcaga cagcacagag agcactgcgg aggctatcca gaagcaggca
    13081 gctatgtgtg ttgatattca gggaaataca gacaatgcag aaagtggaat caaggctatg
    13141 atagatgcct cacgccagac tgatgagacc gtaaagcagg gagctgatgt tgtggaagaa
    13201 ctcaaggagc aggcacacaa cgtggcagag gcaagcaatg ttacagtgtc agttattcag
    13261 agtcttacag ctaaggtaga ggaagtaaag agctttgttg agtcgattat caatatttct
    13321 aaccagacaa atctgcttgc tctcaatgct tcaattgagg cggccagggc aggtgaagca
    13381 ggaaagggct ttgcggtagt agcagatgaa atcagacagc tctcagagca gacaaaggat
    13441 gcatcagcca atattacaga gattataaac aagctcaatg aggacacaag gcgcgccaac
    13501 gagagtatcg agaactcagt atcatcggta gagaagcaga atgagcttat cgacgataca
    13561 agagacaagt ttaatgccat gggagaggcc gttgagctgt tgatgaagga tatcaatgtg
    13621 gctgaggaga gtatcaaaaa gattcttgat tcaacaacag tcatatcaga caatatcaca
    13681 cacctttcag ctaccggaga agaggtcgca gcttcttcaa cagagggact tcgcatggct
    13741 gataccacag tcgagagcat ggcaaagtgc aagaaggtac tggaaaatat ctatatgctt
    13801 gcggatgacc tcaaaaactc tgttgaggag agtgaggaag agcttagtca gaaatttgat
    13861 ttctaagagc agtaatatgt tttttacggc aggagcgcat ttgctcctgc catttttaca
    13921 tattttttat gataaaaata tttacattat tgtaatatat gataaaatat ttctaacacg
    13981 ataacacaat ggctacataa acaataggat atatggagga agttatggac gataacaatt
    14041 atatgaacaa tgacaaccgg tcgtcaggca caccggacaa tacaggtgta ttcggtcagt
    14101 cttcagaaac agcaaatggc tcagacacat acagccagcc gcaggacaca tacaatcagc
    14161 cgacagacac atataatcag tctgtatccc agcaggaaag tgggaatgca tatggacagt
    14221 catcagacca gacaggcagc acagacggat atgtcaataa tccgggcagt gcatatggtt
    14281 atgcaaatca gggtacatac aatcagaatc caaatgccca gggtgcatac aatcagaatc
    14341 caaatgccca gggtacatac aaccagaaca catatgctca gggctcattt aatcagtcag
    14401 cttacggaca gacgcagtat ggccagcagc caaacggaca gggtggctac aatcagaccg
    14461 catacggaca gacagcatac aatcagccac agtatggagc taatccatac aaccagccgc
    14521 caaagaacag caatggcttt ggtatagctt ctctggtact tggaatcgca tctattgtac
    14581 tgttttgtac atgcatcaat tatgtgacag ctctgcttgc tatcattttt ggtattatac
    14641 atatcataaa gggcaacaac aaggtgtttg gtatagttgg tatagcaaca gcagctatct
    14701 cagttatagt cagcatcata ttctgggttg tggcaggctc aaatgcaagc acagattttg
    14761 aaaaggagtt catgaagggc tttgatgagg gctatgagta ttataatgat gacgattcct
    14821 atgaggatta cgattatgat tatgattctt ttgattatga cgattttgat tatcagaatt
    14881 ttgagtatga tggcatttat tagagttaat tattatcaaa ttattacaga atcaattaca
    14941 tcttgacaac agtggctttt cagttatata cttaaacaca gttagtaaaa ggcagtgacg
    15001 aagacagtag agatggcagg aaagtcaaag agagccggga caggtgagag ccggtatgag
    15061 tagagccgtt ttgaaaatca cttctgagcc gcaggctaaa atcgccatac agcgttgcga
    15121 gagtaggttc tgacgtattc cctacgttac aggggcgggt atgcaagggt atttccctta
    15181 agtacattgt aataggtggt atagacgtat caggcacgcg ttcctaatta caggaatgtg
    15241 tgcctttttt tgtgaatatt caataaaccg ggatgtttac gaacaatata gaataaggag
    15301 agatagtcat gtacaacaaa gttgacacca atatgaactt cgttgaacga gagaagaaga
    15361 cagagcagtt ctggagagag aacaatatct tcagaaaatc tatggaaaac agaaaagaag
    15421 gagagacata cacattctat gatggacctc ctacagcaaa cggaaagcct cacatcggac
    15481 atgttgagac acgtaccata aaggatatga ttccaagatt ccagaccatg aagggcaaat
    15541 atgtgccacg taaagcaggc tgggatacac acggacttcc tgtnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn ttctgggctg atatggataa tccatacgtc acatacgatg ataatttcat
    15781 cgagtcagag tggtgggctc tcaaggaaat ctggaacaag ggacttcttt acaagggctt
    15841 caagattgtt ccttactgtc ctcgttgtgg aacaccgctt tctgcacagg aggtggcaca
    15901 gggctacaag acagtcaaag aaaaatcagc agtagtacgt tttaaggtag taggagagga
    15961 tgcatatttc ctcgcatgga ccacaactcc atggacactt ccaagcaacg tggcactctg
    16021 tgtgaaccct gatgacacat atgtaaaggt aaaggctgtt gacggctaca catattatat
    16081 ggcagaggca cttgcagaca aggtgctctc gcagcttctt tcaaaagagg acgcagaagc
    16141 aggcaagaag gcatatgagg tgcttgagac atacaaagga aaggatcttg agtacaagga
    16201 gtatgagccg ctttatgact gcgctaagca ggttgctgat aagcagggca agaagggctt
    16261 tttcgtgaca tgtgacacat atgttactat gtcagacggt acaggaattg ttcatatcgc
    16321 tcctgcattt ggtgaggatg atgccaatgt cggaagaaat tatgaccttc catttgtaca
    16381 gtttgtaaac gacaagggag agcttacagc cgaaactccg tttgccggta tgtgggtaaa
    16441 ggatgcagat cctgaggtat taaaggactt aagcggcaga aagcagcttt ttgatgctcc
    16501 gaaatttgag catgagtatc cacactgctg gagatgtgac aagcctctta tctactatgc
    16561 aagagagtca tggtacatca aggagacagc agtaaaggat gaccttatca gaaacaacaa
    16621 tacagtaaac tggattcctg agtctatcgg ttcaggacgt ttcggaaact ggcttgagaa
    16681 tatacaggat tgggctattt cacgtaaccg ttactggggc acaccactca atatctggga
    16741 gtgtgaatgc ggacacagag agtgtatcgg aagccgtgcg gagctcgctg aaaaagcagg
    16801 agatccaaag gcagcagagg tagagcttca cagaccatac atcgacgctg tgacaatcaa
    16861 gtgtcctgag tgtggcaagg atatgcaccg tgtacctgag gtgcttgact gctggtttga
    16921 ttcaggagcc atgccatttg cacagcacca ctatccattc gagaacaagg aagtatttga
    16981 gaagcagttc ccggccaagt ttatctcaga ggcagttgac cagacacgtg gatggttcca
    17041 ttcactcatg gctgagtcta cactcctttt caacaaggcc ccttatgaga atgtcatcgt
    17101 gcttggacac gtacaggatg agaacggaca gaagatgtc
//
LOCUS       DPUF01000131            5198 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_32146, whole
            genome shotgun sequence.
ACCESSION   DPUF01000131 DPUF01000000
VERSION     DPUF01000131.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_32146"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            136..>671
                     /locus_tag="DHV81_04735"
     CDS             136..>671
                     /locus_tag="DHV81_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93798.1"
                     /translation="MAVKQKILIVDDDNNIAELISLYLTKECYDTKIVNDGEQALIAF
                     EHYKPNMLLLDLMLPGIDGYQVCREIRAKSDVPIIMLSAKGEVFDKVLGLELGADDYI
                     LKPFDSKELVARVKAVLRRFQPAKPEAYASVDIKCVEYPGLIVNLSNYSVTYDGQQID
                     MPPKELELLYFLASSPNQV"
     assembly_gap    672..781
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <782..856
                     /locus_tag="DHV81_04740"
     CDS             <782..856
                     /locus_tag="DHV81_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93799.1"
                     /translation="EKIKDKPTWRIATVWGIGYKFEVK"
     gene            859..2298
                     /locus_tag="DHV81_04745"
     CDS             859..2298
                     /locus_tag="DHV81_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCI93800.1"
                     /translation="MKHTIYTKLLISYLIYGVIAFFIICTFTQHLTTDYIEKQEASNL
                     YREASIIAGDYAAEYFGSSMSLSDFQNHMKIVADYMDAEVWVVSPDGELLMDSDDPSI
                     GIDIQNDSSKPVVINGFDVTDFGSDNYMIGDFYGSFKHKMLSVFSPVNVSYQNIGYIV
                     IHKTMSHITAGVNGFMNISFYTVALIFAVAFILLVMLSRSIYQPITRITKTAKKYAKG
                     DFTAQIDVHSNDEIGYLANTLNYMATELDTLEEDQRKFVSNVSHDFRSPLTSIKGYVE
                     AMLDGTIPVEMQDKYLNIILFETERLNKLTKSLIDLNQFGHHGIHLDIADFDINTMIR
                     TTILTFEGKCSEKGLSFDLLLTGKELFVRGDMVKIQQILYNLIDNAVKFSNNDSSIKI
                     ETSIRNEKVFISVKDHGIGIPKDSLSKIWERFYKSDLSRGKDKRGTGLGLSIVKEIVQ
                     AHGENINVISTEGVGTEFIFTLPLSKKEG"
     gene            complement(2746..>3432)
                     /locus_tag="DHV81_04750"
     CDS             complement(2746..>3432)
                     /locus_tag="DHV81_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93801.1"
                     /translation="IKYTGEATLPLSEDYAGNVTFLFVDGNKRSTEVLDEFKGVTSVF
                     KNVASGILANKPTFDNGSIKLGTDVDVAVMGNDYFDFNSVQLVVASSDTELKTYDIRQ
                     LLAENGTDSADSADTDSITSALSRKDDAKKDGSKNDTENDTSDASKEDDGKLHSLEGT
                     VKIEDLIPVTAASNIKIYVKAVSAQGYTFTYDLFDGIVNQDITDFETTADISGGDYTV
                     TDPAGSKYEP"
     assembly_gap    3433..3623
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3779..5198)
                     /locus_tag="DHV81_04755"
                     /pseudo
     CDS             complement(3779..5198)
                     /locus_tag="DHV81_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516075.1"
                     /note="frameshifted; incomplete; missing start; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
BASE COUNT         1300 a         1127 c         1064 g         1404 t
ORIGIN      
        1 aagaggacaa aaactattgc ggaataccat ctcggtgagt ttggtgaggg cgatgccgga
       61 gtaaccatcg caacctttaa ataacgacaa tgtactttta cggaatataa ataattttta
      121 agaaggagat tagaaatggc tgttaaacag aaaatactga tagtagacga cgacaacaat
      181 atagcagagc ttatatctct atatctgacc aaggaatgct atgacaccaa aatcgtaaat
      241 gacggtgagc aggctcttat cgcgtttgag cactacaagc caaacatgct tcttttggac
      301 ctcatgcttc cgggcataga cggctatcag gtatgccgtg agatacgtgc aaagtccgat
      361 gtgccgatta tcatgctctc cgcaaagggc gaggtatttg acaaggtttt aggcctggag
      421 cttggcgccg acgactatat actcaagccc tttgactcaa aggagcttgt ggcaagagtc
      481 aaggccgtgc tcagacgctt ccagcctgca aagccggagg cctatgcttc cgtagatatc
      541 aagtgtgtgg agtacccggg gcttattgta aatctgtcca actactctgt cacttatgac
      601 ggacagcaga ttgatatgcc gccaaaggag cttgagcttt tatattttct tgcctcatct
      661 ccaaatcagg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 ngagaaaatc aaggacaagc caacctggcg catcgcaact gtctggggca tcggatacaa
      841 gtttgaggta aaataataat gaagcacacc atctacacta agctgttaat cagctatctg
      901 atttacggtg tcatcgcttt tttcattata tgcaccttta cacagcatct caccacagac
      961 tatatcgaaa agcaggaggc ttccaatctc tacagggaag catccatcat tgcaggtgat
     1021 tacgccgccg aatactttgg ctcatccatg tcgctaagcg atttccagaa tcacatgaaa
     1081 atcgtagccg actatatgga tgccgaggta tgggtggtca gccctgacgg tgagctgctc
     1141 atggactcag atgatccgtc aataggcatt gatatccaaa atgacagttc taagcctgta
     1201 gtgataaatg gctttgatgt caccgatttt ggcagcgaca actatatgat tggagatttt
     1261 tacggctcct tcaagcataa gatgctgtcc gtattttccc cggtaaatgt ctcgtaccag
     1321 aatatcggct acatagtcat tcacaagaca atgagccata tcacggcagg tgtaaacggt
     1381 tttatgaata tctcctttta cactgtagcg ctcatttttg cagtggcatt tattctgctt
     1441 gtcatgctga gccgctctat ttaccagcca atcacacgaa tcacaaagac tgcaaagaaa
     1501 tatgcaaagg gtgatttcac cgcccagatt gatgtacatt ccaatgacga aatcggctat
     1561 ctggcaaaca cactcaacta catggcaacg gagctagata cgcttgagga ggatcagcgc
     1621 aagtttgttt ccaacgtgtc acacgacttc cgctcccctc ttacctcgat aaagggctac
     1681 gtggaggcta tgctggacgg aaccatacct gttgagatgc aggataaata tttaaatatc
     1741 atactttttg agaccgagag gcttaataag ctcaccaaga gtcttatcga tctgaaccag
     1801 ttcggtcatc acggcataca cctggatatt gctgattttg acatcaatac aatgatccgc
     1861 acgacaatcc ttaccttcga gggtaaatgc agtgaaaagg gcttaagctt tgacctgctg
     1921 ctcaccggaa aagagctttt cgtgcgcggt gatatggtca aaatccagca gattctttac
     1981 aacctgattg acaacgctgt gaagttttca aataatgatt cgagtatcaa aattgagacg
     2041 agtatcagaa atgaaaaagt gtttatatca gtaaaggatc acggtatcgg tataccaaag
     2101 gacagccttt caaagatctg ggagcgcttc tacaagagtg atttatcccg cggaaaggat
     2161 aagcgcggta cgggacttgg cctgtctata gttaaagaaa tcgtgcaggc gcatggtgag
     2221 aatataaatg tgataagtac tgagggtgtg ggaactgagt ttatatttac gctgccactc
     2281 agcaaaaaag aagggtgagg cgcagctcat catatgatag gtgtggtggg cgcctcgctt
     2341 tttagtttgt gaggctgccg gcatagcaca cagtatattc ctcagaaaaa tgtgaagtca
     2401 gccgcggttg tttgtccggg caggttacga ttttatttat gttttctttc ttctttaatt
     2461 tatggaactc cgcggcagtt cacatttttc ttcggaacac actgtatgct ctccggctaa
     2521 ttcaggttta cgagcgaggc gctttgctac cttgcattct ggcagtgccg gacttatttc
     2581 actggtgtga aattttgtcc ggcacttttt tcttcttaaa atttaaagct catttcactt
     2641 atgcgaaatg agcttattta atagttatat attgtttata aatattctga gccgccttga
     2701 aaacacaaga aaaactgatt ttaaataatt acgcttatat catatttacg gttcatattt
     2761 gctgccggcc gggtcggtta ctgtgtagtc gccgccggat atgtcggctg tagtttcaaa
     2821 gtctgtgatg tcctgattta cgataccatc gaacaggtca tatgtgaagg tgtagccctg
     2881 tgccgatact gctttgacat atattttgat attgcttgcg gctgtgacag ggattaagtc
     2941 ttcgattttg actgtgcctt cgaggctatg gagcttaccg tcgtcctctt ttgaggcatc
     3001 agaggtatca ttttccgtgt cattctttga gccatctttt ttggcatcat ccttccggct
     3061 taaggcggat gtgatggagt ctgtgtctgc agagtctgca ctgtctgtgc cgttttctgc
     3121 caacagctgt cggatatcgt atgtctttag ctctgtatct gagcttgcaa ctacgagctg
     3181 cacactgtta aagtcaaagt aatcatttcc catgaccgct acatctacat ctgtgccaag
     3241 ctttatgctg ccattgtcaa aggttggttt attggcaagt atgccggatg ctacattttt
     3301 gaacactgat gtcacacctt taaactcatc gagcacctct gtgcttctct tatttccgtc
     3361 aacaaacaga aatgtcacat tgcctgcata gtcctcagac agtggaaggg tggcctcacc
     3421 cgtatacttg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnngaaagta tcaccgatgt gttgtctgag gtgtatgaca
     3661 tctgtgtctc gagctttttt atcctgttat taagcaatac tgcccccgca ataagtgcgg
     3721 cgagcagtat tatggtaatc gcattgaaaa tatttttttt caaattcata ttatagtgtt
     3781 attcctcatc ttcctctgca gctacagcta ttccaagctc ctcaagctgc tttggatcta
     3841 cagttccggg tgcctctgac atgaggtccg aagcgtcctt taccttaggg aatgcaataa
     3901 cctccttgat attgtctgta tgcagcatat gcatgcagat acggtcaaca ccgaaggcaa
     3961 gtcctgcgtg tggcggtaca ccatatgaga atgcatcaag caggaatcca aactgctcat
     4021 gtgcacgctc ctttgtgaag ccaagcacct caaacatctt ctcctgnnat acgcactgag
     4081 cctccaccga gctctgtacc attgagtacg atatcatatg cctttgcacg aactgcgccc
     4141 ggatctgagt ctatcttgtc ccaatcctcc tccataggca tagtgaatgg atgatgcatt
     4201 gccataaatc tgttttcctc atcagaccac tcaaggagtg ggaactctgt tacccagagg
     4261 aaattgtact tgttctcatc aatcaggcca agctcagctc cgagcatgag acgaagcgct
     4321 ccaagtacat tccatacgat tttattcttg tctgctgcga aaaggagtaa atctcccggc
     4381 tttccgttca tctttgcaac aagtgcatca agctcagctt ctgtcataaa cttggcaaat
     4441 gatgacttgt atgtgccatc ctcattgatg cacagataag caagtccctt tgctccacag
     4501 ccctttgcgt gctctacaag cttgtcaatc ttcttacgag gcatctttgc ctgtccctct
     4561 acattgatac cacggacaga gccaccgttt tcaagagctc ctgtgaatac tccaaagcca
     4621 cagtccttta ccacttccga aacatcgcaa agctccatac caaagcgtgt atcaggcttg
     4681 tcagaaccaa atctgtccat agcctcctgc caaggcatac gcttaaacgg aatctcaaca
     4741 tccacattta tgacctcttt aaaaaggtgc tttaacagtc tctcatttac ctcgattaca
     4801 tcatcgatat ctacgaatga aagctccata tctgcctgtg taaactcagg ctgtctgtct
     4861 gcacgcagat cctcgtcacg gaagcatcgt gcaatctgga aatatctgtc atatcctgaa
     4921 gccatcagaa gctgcttaaa gagctgtggt gactgaggaa gcgcataaaa atgtccgtgg
     4981 tgtatacggc ttggcacgag ataatctctg gcaccctctg gtgtagactt gcaaaggatt
     5041 ggtgtctcta tctccaaaaa tccctcgttt gccataaagt cacgcatcac tcgaagcaca
     5101 ttgcttctga gcatgagatt tctctggata tcagggcgtc gaagatccag ataacgatat
     5161 ttaagtctga tgtcttcgct ggtctttgaa ttctcctc
//
LOCUS       DPUF01000132            2959 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_2595, whole
            genome shotgun sequence.
ACCESSION   DPUF01000132 DPUF01000000
VERSION     DPUF01000132.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_2595"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(1..1791)
                     /locus_tag="DHV81_04760"
                     /pseudo
     CDS             complement(1..1791)
                     /locus_tag="DHV81_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516732.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     assembly_gap    1497..1588
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1862..>2959)
                     /locus_tag="DHV81_04765"
     CDS             complement(1862..>2959)
                     /locus_tag="DHV81_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743114.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCI93802.1"
                     /translation="NNIYNSEGGAHITGFKTAFTGIINSYARELGILKDKDANFNGAD
                     VRNGMTAVVSVKHPDPRFEGQTKTKLDNQDANRATAKVTNDEVPLYFDKNLEVLKTVI
                     SCAEKAAKIRKAEEKAKTNLLTKQKFSFDSNGKLANCESRDASKCEIFIVEGDSAGGS
                     AKMARNRMFQAIMPIRGKILNVEKASIDKVLANAEIKTMINAFGCGFSEGYGNDFDIT
                     KLRYDKIIIMADADVDGAHISTLLLTLFFRFMPELILEGHVYVAMPPLYKAIPSKGEE
                     KYLYDDAELDKYRKTHTGKFTLQRYKGLGEMDADQLWDTTLNPATRMLKRIEIEDGRL
                     ANDVTSLLMGSDVPPRKEFIYEHAHDAELDV"
BASE COUNT          685 a          741 c          515 g          926 t
ORIGIN      
        1 aatttgccgt aaggcaggtc taataccttg acaagatgca tgtttgcctt gtttgtaaaa
       61 atgcacagct tatctatatt tttgaccttc aatatcagct tgttctctgc gtccgcagtc
      121 tctttgtttc tctcataggt tgaagtatct actgtcttca catagccgaa gcgatccata
      181 agcagcacta catccgatac ctcaagctct tttttgacca caacagcttc ctcgagattg
      241 tcaagctccg tgcgtctatc ccttgcatac tccttgcgga acgctttaag ctccttcata
      301 atgacctttg ccatggacga gcgatgctct aaaatatcgc tgtactctgc aatctttgcc
      361 ctggtctcct cgtgctcctt aatcagagcc tcaatttcga gtccaatcag cttgtacagg
      421 cgcatttcaa ggatagcagc tgcctgacgc tcagtaaagc aaagctgggc tgccatggct
      481 tctgaagcct ttgacttgaa tttaatcccc tctgtctctc cgtttatgag acatgccttt
      541 gccattttct ggtcacggct tcctcttaat atttcaataa tcagatctat cacgttacag
      601 gcctttatga gcccctgctg tatttcttct ttctcctgct cctttgcaag aagtgtctca
      661 tactttcttt ttgcaatctc atactggaaa ttgacatgat gccttatgat gggtacaaga
      721 ccaagcgtct caggtcttcc atttgcgaca gccagcatat ttacaccgaa agtgtcctcc
      781 agctttgtct ttttatacaa aaggttctta agtgcctcaa catcagcacc ttttttaagc
      841 tctaagacaa ttcttattcc ctctttcgat gactggttgg ttatatccac aatatccgtt
      901 gtcgcttttg actcgacaag tgaatagaca tcatttaaga attttcctat attggcacca
      961 atcattgtgt atggaatttc cgtaataaca agcttgtcct ttcctgcctt gcccttctct
     1021 atttcgattc gtccacggat ttttatcttt ccctgtccgg tctcatatat tgcggcaaga
     1081 tcatcctgat ttgcaacaat acctcctgtc ggaaaatcag gtccctttat atactgcatc
     1141 atctcggctg tggtgatatc aggatttttc atataagcaa tcacaccgtc caccacctcg
     1201 ccaaaattgt gtggcggaat gcttgtcgcc atgccgactg ctataccctc agctccattt
     1261 atcaaaaggt tcggcacctt gaccggaagc acctctggct ctttttctgt ctcgtcgtag
     1321 tttggaatga aatcaaccac atttttgtca agatcagcaa gatatgcttc ctgtgtgatt
     1381 ttttgaagtc ttgcctcagt ataacgcatg gcagcggctc cgtctccctc gattgagcca
     1441 aaattaccgt gtccatcgat aagcggcatg ccctttttga agtcctgtgc caaaacnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta tggcttgtcg tacctgatgc ccagctcatg
     1621 catatcataa agtgtacgcc tttgtacagg cttaagccca tcgcgcacat ccggcagggc
     1681 tcttgctata atgacactca tcgcatagtc aatatatgat ttctgcatca gctccgaata
     1741 ttcggtctga attatattgt tgtctacatt gttatctata tttctatcca tgtctttctc
     1801 cgattataaa tactgcttaa ctattcagaa ccactgagaa caaaaagcag aagcgtcatg
     1861 cttacacatc cagctccgcg tcatgcgcat gctcgtatat gaactctttt ctcggtggca
     1921 catcactacc cataagcagt gatgtcacat catttgccag gcgcccatcc tctatttcaa
     1981 tacgctttag catccgtgtt gcgggattta gtgtggtatc ccagagctga tccgcatcca
     2041 tctctccaag acccttatat ctttgcaacg taaacttgcc tgtatgtgtc tttctgtact
     2101 tgtcaagctc cgcatcgtca tacaaatatt tttcctcgcc ctttgacgga atagccttgt
     2161 aaagcggagg catggcgaca tatacatgtc cctctaaaat aagctcaggc ataaacctga
     2221 agaacagagt caaaagcagt gtggagatat gagctccgtc cacatcggca tcggccataa
     2281 tgatgatttt gtcatagcga agctttgtta tatcaaaatc atttccatat ccttccgaaa
     2341 atccgcagcc aaacgcattt atcattgtct ttatttcagc gtttgccagt accttatcaa
     2401 tacttgcctt ttcaacattc aaaatcttgc ctcgaatcgg catgattgcc tgaaacatac
     2461 ggtttcttgc catctttgcc gagcctccgg ccgaatctcc ctcgactatg aaaatctcgc
     2521 acttagaagc atccctgctc tcacagtttg caagctttcc gtttgaatca aaggaaaact
     2581 tctgctttgt gagcagattg gtctttgcct tctcctcagc ttttctaatc tttgcggcct
     2641 tctcagcaca tgatattaca gtctttaaga cctcaagatt cttgtcaaaa taaagcggta
     2701 cctcgtcatt cgtcaccttg gcagtagcac ggtttgcatc ctgattgtca agctttgtct
     2761 ttgtctgacc ctcgaatcta gggtcaggat gctttactga cactacagca gtcatgccgt
     2821 ttctgacatc tgctccgttg aaattcgcat ccttgtcctt taatatacca agctctctgg
     2881 catatgaatt tataatacct gtaaaggctg tcttaaagcc tgtgatatgc gctccaccct
     2941 cagaattgta tatattgtt
//
LOCUS       DPUF01000133            3221 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_5036, whole
            genome shotgun sequence.
ACCESSION   DPUF01000133 DPUF01000000
VERSION     DPUF01000133.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_5036"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="DHV81_04770"
     CDS             <1..530
                     /locus_tag="DHV81_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HCI93803.1"
                     /translation="VKIKLNGKLITDSVDQDMVLLDFCRKHNCFSVKRGCESGNCGLC
                     TVLLDGKPVLSCSTPIGRADGRAVDTLEGLEEEAKQFTGFFAAQGADQCGFCNPGYIV
                     NIVALLRENPDPTDEEILEYLSGNLCRCTGYQSQLRSIRNFLNSKKEGAPLNLEAGVY
                     GEDIGKEWQDEHKSN"
     gene            511..>2822
                     /locus_tag="DHV81_04775"
     CDS             511..>2822
                     /locus_tag="DHV81_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HCI93804.1"
                     /translation="MSTNQTNENSFNRARRDYHAVGKCIPKKDSSQLLLGKPVFTDDI
                     TPRDALVIKLLRSPYANAIVEDVKTDIALKVPGMVAVYTWEDVPKKRFSIAGQTFPEP
                     SPYDRLILDRHVRSVGDAVAIVVGESEKAVDKALNMIKVKYTVLEPVLDFRKSLDNKV
                     LVHPEDDWSSSIDTGDVKRNLIHHEEDEHGNVEKILSECDEIIEHTYRIKAAQQAYME
                     TCRAYCEIDRYGRLHCISSTQIVFHLRRILANALDIPKSMVRAEKPRIGGGFGAKQTA
                     VCEVYPAFVTWRTKRPSKIIYSRKECQTIASPRHEMEVTVRLGAMKDGHIRAIDLYTL
                     SNGGAYGEHSTTTVGLSGHKSLPLYTGGCEATRFSCDVVYTNVQAAGAYRGYGATQGI
                     FALESTVNELAEKLHIDPVELRLKNIVREGMFMPAYFGETANACALDRCIMHCADNFH
                     WAEKYPVRDMGNGKVRAAGMALAMQGSCISNVDVGSCTLKLSDCGTYNMMIGAADMGT
                     GCDTILAQMAAEVLDCEPDDITVFGADTDASPYDSGSYASSTTYITGMATQNAALELR
                     ENIKKIGAQLLGCAASEVDFDGKEVYIINPDGADNGAVSVSLSDIATKSQVNNTIPVD
                     VTATYSSPVSPPPYMVGMVEIELDKETGAVKILDYQAVVDCGIPVNPNLARVQTEGGI
                     GQGIGMALYENVTYNAGGKIAENDFMQYKIPTRQDVGHINVEFETSYEPSGPFGVKSI
                     GEIVINTPAPALAHAIYRATGVWHRTVPFTP"
     assembly_gap    2823..2893
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2902..>3221
                     /locus_tag="DHV81_04780"
     CDS             2902..>3221
                     /locus_tag="DHV81_04780"
                     /inference="COORDINATES: protein motif:HMM:PF12804.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HCI93805.1"
                     /translation="MIHIIYMAAGNSRRFGSNKLFYELDGKPMYRHLLERLIEIKDRY
                     NKLKNAESNNPVIDITVVTRYREILDYCSSIPDCHAVLSPDSEKGISYTIKAGIMAVQ
                     EQKKT"
BASE COUNT          920 a          656 c          839 g          735 t
ORIGIN      
        1 atgttaaaat aaaattaaat ggaaagttaa taaccgactc ggtagaccag gatatggtcc
       61 ttttagactt ttgccgtaag cacaattgct tttcggtgaa gagaggctgt gagtcaggca
      121 actgcgggct ttgtacagtg cttctggatg gaaagcctgt gctgtcatgc agcacgccga
      181 taggcagggc agacggcaga gcggtggaca ccctcgaggg acttgaggag gaagctaagc
      241 agtttacagg attttttgct gcacagggag cggatcagtg tggcttctgt aatccgggtt
      301 atattgtaaa tattgtggcg cttctgagag aaaatcctga cccgactgac gaggaaattc
      361 ttgaatatct ttcaggaaat ctgtgccgtt gtacaggata tcagagtcag ctgcgctcaa
      421 tcagaaattt cttaaacagt aagaaggagg gcgcacctct taatcttgag gcaggcgtct
      481 atggagagga tatcggaaag gagtggcagg atgagcacaa atcaaactaa cgaaaacagc
      541 ttcaaccgcg ccagaaggga ttaccacgcg gttggaaaat gtatacctaa aaaggattcg
      601 agccagcttt tactcggaaa gccggtattt accgatgata tcacaccgcg tgatgcgctt
      661 gtcataaagc ttcttagaag cccgtatgca aatgcaattg tcgaggatgt aaagacagat
      721 atcgctctta aggtgccggg catggtcgca gtatacacct gggaggatgt tccgaaaaag
      781 cgtttttcta tcgcaggcca gacttttccg gagccgtcac catatgacag attaatcctt
      841 gatcgccatg tgagaagcgt aggtgacgcc gtcgctatag tggtcggaga atctgaaaag
      901 gcggtggaca aggctcttaa catgataaaa gtaaagtaca cggtgcttga gccggtgctt
      961 gacttcagaa agtcgctgga taacaaggtg ctcgtgcatc ctgaggatga ctggtcatcc
     1021 agcatagata caggcgatgt aaagcgcaat ctcattcacc acgaggagga tgagcatgga
     1081 aacgtggaga aaattctgtc agagtgtgat gagataattg agcacacata tcgcataaag
     1141 gcagcgcagc aggcatatat ggagacctgc agggcatact gtgagataga cagatatggc
     1201 agacttcact gtatcagctc gacacagata gttttccacc tgcgccgaat actggcaaat
     1261 gcactcgata ttccaaagtc tatggtgcgc gctgaaaagc cgcgtatcgg aggaggcttt
     1321 ggtgccaagc agacagcggt ctgtgaggtg tatccggcat ttgttacctg gaggacaaag
     1381 cgtccgagca agataatcta cagcagaaaa gagtgtcaga ctatcgcgtc accgcgtcat
     1441 gagatggaag tcaccgtgcg tctcggagcg atgaaagatg gtcatataag ggcgatagat
     1501 ctttatacgc tgtcaaatgg tggagcatac ggagagcata gtactacaac agtaggactt
     1561 tccggtcata aatcgcttcc gctttataca ggaggctgcg aggcaaccag attttcatgt
     1621 gatgttgtgt atacaaacgt gcaggcggca ggagcatata gaggctatgg agccacacag
     1681 ggtatatttg cacttgaatc aacggtcaat gagctcgcag aaaagctgca tatagacccg
     1741 gttgaacttc gtttgaaaaa tattgtacgc gagggcatgt ttatgccggc atattttggc
     1801 gagacagcca atgcatgtgc actcgacaga tgcataatgc actgtgcgga caatttccat
     1861 tgggcagaaa agtatcctgt ccgcgacatg ggaaatggca aggttcgtgc ggcaggcatg
     1921 gcacttgcaa tgcagggctc ctgcatatcc aatgtagatg tcggctcgtg tacattaaag
     1981 ctttcagact gtggcaccta taatatgatg ataggtgcgg cagacatggg aacaggctgt
     2041 gatacaatac ttgcgcagat ggcggctgag gtacttgact gcgagccgga tgatataaca
     2101 gtgtttggag cggatacaga tgcttctcct tatgactcgg gttcatatgc ctcatcaacc
     2161 acctacatca caggaatggc cacacagaat gcagcacttg agcttcgtga aaatataaag
     2221 aaaatcggtg cacagctgct gggctgtgct gcatcagagg tggatttcga tggtaaagag
     2281 gtgtatatca taaatccgga cggtgcagac aatggggcag tttccgtaag tctttccgat
     2341 atagcaacaa aatcacaggt caacaacacg atacctgtag atgtcaccgc cacatattca
     2401 tcacctgtct caccgccacc atacatggta ggcatggtcg agatagagct tgataaggag
     2461 acaggagcag ttaaaatact tgattatcag gctgtcgtgg actgtggaat cccggtcaat
     2521 ccaaaccttg cgcgtgtaca gacagagggt ggcataggtc agggaatcgg catggcactt
     2581 tatgagaatg tcacctacaa tgccggtggt aaaatcgcag aaaatgattt catgcagtac
     2641 aagataccga cacgacagga tgtgggacat atcaacgtcg aatttgagac aagctatgag
     2701 ccttcaggcc catttggagt caagtcaatc ggcgagatag ttatcaatac accggctcct
     2761 gcgctggcac atgccattta tcgcgcaaca ggcgtatggc acaggacagt tccgtttacc
     2821 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnagagtga aatgattcat atcatataca tggcggctgg aaacagccgc
     2941 cgttttggaa gtaacaagct gttttatgag ctggatggaa aacctatgta caggcatctg
     3001 cttgaacgtt tgatagaaat taaagataga tataataaat taaaaaatgc agaatcgaat
     3061 aacccggtca tagatataac cgtcgttaca cgttatcggg aaatactgga ctactgctca
     3121 tctatccccg attgccatgc agtcttaagt ccggacagcg aaaaaggcat atcctacacc
     3181 ataaaagcag gaattatggc ggtgcaggag cagaaaaaga c
//
LOCUS       DPUF01000134            4708 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_32805, whole
            genome shotgun sequence.
ACCESSION   DPUF01000134 DPUF01000000
VERSION     DPUF01000134.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_32805"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="DHV81_04785"
     CDS             <1..189
                     /locus_tag="DHV81_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin HicA"
                     /protein_id="HCI93806.1"
                     /translation="RKVLESYGYVMNAPKGGSSHYTFRKAGCQPITIPKHEPIKKVYV
                     EMVKQIVESEAVNDENAE"
     gene            173..538
                     /locus_tag="DHV81_04790"
     CDS             173..538
                     /locus_tag="DHV81_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HCI93807.1"
                     /translation="MKTLNDYFTMNYRMEIVEDKDEGGFVVSFPELPGCITCGETVES
                     AVANALDAKKAWLEAAMEDGIEIHEPDSLEDYSGQFKLRIPRSLHRSLAEHSKKEGIS
                     MNQYCVYLLSRNDAVYSKL"
     gene            complement(697..1542)
                     /locus_tag="DHV81_04795"
     CDS             complement(697..1542)
                     /locus_tag="DHV81_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCI93808.1"
                     /translation="MLKQIKNFLIMTLSITIMAIGVYYFKFPNNFTFGGVTGAAVVFA
                     KITPLSASMFSTIVNMLLLVVGFFFLGKDFAIKTTYATILLSVELLVLEKLDPLTHPL
                     SNEPMLELIFAIALPAIASALLFYVGASSGGTDVIAMIVKKYAHVENIGMALFLTDLV
                     MIIIACFVFDIKTALYSFVGLVVKSFMIDAIIENIMLRKSIMLICDDKDVICDYITNT
                     LEKSATYVEARGAYTGERNYIVFSTLTKKQAIELRSFIHQNKLHAFMSVMSTSEVFGK
                     GFSSL"
     gene            1844..>2754
                     /locus_tag="DHV81_04800"
     CDS             1844..>2754
                     /locus_tag="DHV81_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCI93809.1"
                     /translation="MDYKNSGVDIEAGYKSVELMKKHVKETMRPEVLGGLGGFSGAFS
                     LASIKDMEDPVLLSGTDGCGTKVKLAFIMDKHDTIGIDAVAMCVNDVACAGGEPLFFL
                     DYIACGKNYPEKIATIVSGVAEGCKQSGCALVGGETAEHPGLMPEDEYDLAGFAVGVV
                     DRKDIITGEGLKDGDVLIGMASTGVHSNGFSLVRKIFKMDKETLNTYHEELGTTLGEA
                     LLAPTRIYVKALKAVKDAGVTVKACSHITGGGFYENIPRMLIDGKRAVVEKNSYPVPP
                     IFKMMAREGNVEEQMMYNTYNMGLGMIV"
     assembly_gap    2755..2872
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2914..3540
                     /locus_tag="DHV81_04805"
     CDS             2914..3540
                     /locus_tag="DHV81_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCI93810.1"
                     /translation="MKIAVCVSGGGTNLQAIIDAIDNGTITNTQIEVVISNNADAYAL
                     ERAKKAGIKAVCISPKSYESRAAFNEDFLKQLNSYHVDLVVLAGFLVVIPPEMIKQYR
                     NRIINIHPSLIPSFCGTGYYGLKVHEGVLARGVKVTGATCHFVDEGTDTGPIILQKAV
                     EVKQGDTPEVLQRRVMEQAEWKIMPHAIDLIANGRVSVEDGYVIIDEK"
     gene            3636..>4708
                     /locus_tag="DHV81_04810"
     CDS             3636..>4708
                     /locus_tag="DHV81_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCI93811.1"
                     /translation="MKVLVVGSGGREHAICRAVAKSPRVDKIYCAPGNAGIAALAECV
                     PIGAMEFDKLVSFAKDNAIDFTIIGMDDPLVGGIVDEFEKAGLKCFGPKKNAAILEGS
                     KAFSKDLMKKYNIPTAAYENFTDAKEALKYLETAKFPIVLKADGLALGKGVLICNTLE
                     EAKEGVRTIMEDKKFGTAGNTMVVEEFMTGREVSVLSFVDGKTIKIMSSAQDHKRAGD
                     GDTGLNTGGMGNFSPSPFYTNEVDDFCKKYIYQATVDAMAKEGRPFTGVIFFGLMLTE
                     DGPKVLEYNARFGDPEAQVVLPRMKNDIIDVMEACVDGTLDTIDLQFEDNACVCVVLA
                     SDGYPVKYEKGFKITGFEKFDGK"
BASE COUNT         1424 a          785 c         1154 g         1227 t
ORIGIN      
        1 cgtaaggtat tggaaagcta tggatatgta atgaatgccc caaagggcgg aagcagtcat
       61 tacacgttca gaaaagccgg atgtcaaccg attacgatac cgaagcatga acctatcaaa
      121 aaagtgtatg tagagatggt aaagcagatt gtggaaagtg aggcggtgaa tgatgaaaac
      181 gctgaatgat tattttacaa tgaattatcg tatggaaatt gtagaggata aggatgaggg
      241 tggttttgtg gtttcatttc cggagctccc gggatgtatt acctgcggag aaacagtaga
      301 atccgctgtc gcaaacgctt tagatgccaa gaaagcatgg ttagaagctg ctatggaaga
      361 tggtattgaa atccatgagc cggacagctt agaggattat tctggacagt ttaaacttag
      421 aattccacga agtcttcatc gttcacttgc agaacattct aagaaggaag gtatcagcat
      481 gaatcagtat tgtgtatatc ttctttccag aaacgatgca gtttattcaa aattatagac
      541 taaatgaaat agggggtgtt ctgttatgtt ttttcttata gcgaaggata atgaatgggc
      601 tggattgctt agtagtctgg atatgttttc tgatgatttt atggaagaag gacggataca
      661 gcctgaagta caggtaaggg aagatttcta atgaatttac agagaagaaa atcccttacc
      721 aaatacttca ctggtgctca tcacagacat aaaagcatgc agcttattct gatgtatgaa
      781 gcttctaagc tcaatggcct gcttctttgt gagagtgctg aaaacgatat agtttctctc
      841 accggtgtaa gcacctcgtg cctccacata tgtggcactt ttctccagcg tgttagtaat
      901 atagtcgcat atcacatcct tgtcatcgca gataagcatt atggattttc tgagcatgat
      961 attttcgatg attgcatcaa tcataaatga tttcacaaca agtccgacaa atgaatacag
     1021 agcagtcttt atgtcaaata caaagcaggc gatgataatc atgacgaggt cggtgagaaa
     1081 taaagccatg ccaatatttt ctacatgtgc atattttttt acaatcatgg cgatgacatc
     1141 ggttccgcca cttgaagccc ccacatagaa aagcagtgca gatgcgattg caggaagagc
     1201 gatggcaaaa atcagctcaa gcattggctc gtttgagaga ggatgagtga gcgggtcaag
     1261 cttttcaagc accagaagct caacagacaa aagtattgtg gcataggtgg tcttaatagc
     1321 aaagtctttg ccaaggaaga aaaagcccac tacaagcaga agcatgttca caatagtaga
     1381 aaacatactc gctgagagag gtgttatctt tgcaaaaaca actgcggcac cggttacgcc
     1441 accgaaggtg aagttgtttg ggaacttgaa atagtataca ccgattgcca tgatggtgat
     1501 actgagtgtc attatcagaa aattttttat ctgtttaagc atttgttgtt ctcctttatg
     1561 atttgacagc ttgtcaggta tttattgcag tgctgtgtat catagctttc ttttgtatta
     1621 ttaatcgttc aaatctaaat atttatttgc atggatagtg taggacgatg gatgtctgat
     1681 gtcaatataa tccgtgaaat aaattataag tattatttct ataaaagata tattatatta
     1741 gtaaaactaa taagaataat tacaatatat aattggaaaa tttagcacta tgaaggtata
     1801 ctataggtat tgaacgaatt gcagattatt aggaggaaca ataatggatt acaagaattc
     1861 aggtgttgat atcgaggcag gttacaagtc agtagagctt atgaaaaagc atgtaaagga
     1921 gactatgaga ccggaggtgt taggtggtct cggaggcttc tcaggagcgt tctcacttgc
     1981 ttccatcaag gatatggagg atccggtgct tttatccgga acagatggct gcggaactaa
     2041 ggtaaagctt gctttcatca tggacaagca tgatacaatc ggaatcgatg cagttgccat
     2101 gtgtgtaaat gatgtggcat gtgcaggcgg tgagccatta tttttccttg attatatagc
     2161 atgtggaaag aactatccgg agaagattgc tacaatcgta agcggtgtgg cagagggctg
     2221 taagcagtca ggctgtgcac tcgttggtgg tgagacagca gagcatccgg gtcttatgcc
     2281 tgaggatgag tatgaccttg caggctttgc agttggtgtg gttgacagaa aggacatcat
     2341 cacaggcgag ggattaaagg atggtgatgt acttatagga atggcatcaa caggcgtaca
     2401 ctcaaatgga ttttcacttg tcagaaagat tttcaagatg gacaaggaga cattaaatac
     2461 atatcatgag gagcttggca ctacacttgg agaggcactt cttgccccta caagaatcta
     2521 tgtaaaggct cttaaggctg taaaggacgc aggagtcaca gtaaaggcct gcagtcatat
     2581 tacaggcggc ggcttctatg agaatatccc acgtatgctt atcgatggca agcgtgccgt
     2641 agttgagaaa aacagctacc ctgttccacc aatctttaaa atgatggcta gagagggaaa
     2701 tgttgaggag cagatgatgt acaataccta caacatggga cttggaatga ttgtnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntatattgt
     2881 taataattgt atacatcgtt gcgaggaaaa agaatgaaaa ttgcagtatg tgtatcgggt
     2941 ggcggcacca atcttcaggc catcatagat gcaatagata acggaacaat tacaaacaca
     3001 cagatagagg ttgttatcag caacaacgca gatgcatatg cacttgagag agcaaaaaaa
     3061 gcaggaataa aggcagtatg catctcgcca aagagctatg aaagcagagc tgccttcaat
     3121 gaagatttct taaagcagct taactcatat catgtggatt tagtcgtact ggcaggtttt
     3181 ctggtagtga ttccacctga gatgataaag cagtacagaa acagaatcat aaatatccat
     3241 ccatcgctca taccatcttt ctgcggcaca ggctactatg gtcttaaggt gcatgaagga
     3301 gtgcttgcac gcggtgtgaa ggtgacaggt gccacctgcc actttgtgga tgaaggcaca
     3361 gacacaggcc ctattatttt acagaaggca gtcgaggtaa agcagggcga cacacctgag
     3421 gtgcttcagc gcagggttat ggagcaggct gagtggaaaa tcatgccaca cgctatcgac
     3481 ctgatagcaa atggcagggt gtcagtagaa gacggatatg ttattataga tgagaagtaa
     3541 tgacagtgct gtaagtaatt agtatgtaaa cacaggtaga ttatgattac cggacaatga
     3601 aaagcttatg tatagcttag gtataggagg caaaaatgaa ggtattagta gtaggaagcg
     3661 gcggaagaga gcatgcaata tgcagggctg ttgcaaagag tccacgcgtt gacaagatat
     3721 actgtgcacc gggcaatgca ggtatagcag cacttgcaga gtgtgtgcca atcggtgcga
     3781 tggaatttga taagctcgta tcatttgcaa aggataatgc cattgatttt acaatcatcg
     3841 gcatggatga tccgcttgta ggtggtatcg ttgacgagtt tgaaaaggca gggctcaagt
     3901 gcttcggacc aaagaagaat gcagctattc ttgagggctc aaaggcattt tcaaaggacc
     3961 tcatgaaaaa atataatatc cctactgcgg catatgagaa ttttactgat gccaaagagg
     4021 ctcttaaata tttagagaca gcaaagttcc ctatcgtgtt aaaggcagac ggacttgctc
     4081 tcggaaaggg tgtgcttatc tgcaatactc ttgaggaggc aaaagagggt gtcagaacca
     4141 tcatggagga taagaagttc ggaacagccg gaaacaccat ggtagttgag gagttcatga
     4201 caggccgtga ggtttcagtg ctttcatttg tagacggaaa gactatcaag attatgtcat
     4261 ctgcgcagga tcacaagcgt gcaggcgatg gagatacagg tcttaataca ggcggcatgg
     4321 gcaatttctc acctagtccg ttctatacca atgaggtcga tgatttctgc aagaagtata
     4381 tttatcaggc aactgtagat gctatggcta aagagggcag accttttaca ggagttatct
     4441 tcttcggact catgctcaca gaggatggac caaaggtgct tgagtacaat gcaagattcg
     4501 gagatcctga ggcacaggtt gtgcttcctc gtatgaagaa tgatatcatt gatgtaatgg
     4561 aggcctgtgt agacggaact cttgacacaa tagatcttca gttcgaggac aatgcatgcg
     4621 tatgcgtggt tctggcatca gacggatatc ctgtcaagta tgagaagggc tttaagataa
     4681 ctggctttga gaagtttgac ggcaaaga
//
LOCUS       DPUF01000135            5116 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12748, whole
            genome shotgun sequence.
ACCESSION   DPUF01000135 DPUF01000000
VERSION     DPUF01000135.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12748"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(24..518)
                     /locus_tag="DHV81_04815"
     CDS             complement(24..518)
                     /locus_tag="DHV81_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCI93812.1"
                     /translation="MTADETLNELLVCLFKDLTEIEGRCLITDEFSDISINDMHIIEA
                     IGVDEPKRSGTVAKLMSITLGTLTKAIDGLTRKGYVNRIRSEEDKRVVKLSLTGKGKS
                     AYYHHEQFHRHMIEHIKDGLSDDEMKVLITSLAKLSDYFKVVYSDDEENQYQNWDNIN
                     EDNK"
     gene            complement(531..1499)
                     /locus_tag="DHV81_04820"
     CDS             complement(531..1499)
                     /locus_tag="DHV81_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HCI93813.1"
                     /translation="MKIVGTGSCLPQRVVSNDDLAAIMDTSDEWISSRTGIRNRHIAT
                     TETTTSLACDAVKSALQDAGIDGSEIDLIIAASVSTDKIVPSLACQIQAEIGAGSAVA
                     FDINAACSGFLFGLATADAYFKTGRFHKTVVVGAEVLSKIMDWNDRSTCVLFGDGAGA
                     AVVTDEGDAFKGFVQGSDGKGGEALDGDNRPVVNPFTDNGKQSALGGYVTMDGPAVYK
                     FAVKTVPKAINELLDEIGWSVDDVDVYVLHQANIRIIESVAKRLKQPMDKFPTNLQHC
                     GNISAASVPILLDKVRKDGMIKHEEKIILSGFGAGLTWGACAIEWQ"
     gene            complement(1518..3307)
                     /locus_tag="DHV81_04825"
                     /pseudo
     CDS             complement(1518..3307)
                     /locus_tag="DHV81_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515909.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
     assembly_gap    2655..2732
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3325..3753)
                     /gene="fabZ"
                     /locus_tag="DHV81_04830"
     CDS             complement(3325..3753)
                     /gene="fabZ"
                     /locus_tag="DHV81_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HCI93814.1"
                     /translation="MSIMNTQQIMDILPHRSPFLLIDTVEELEPGVKCVAKKNVTMNE
                     PHFMGHFPGNPVMPGVLIIEALAQTGAVAILCQDEWKGKTAYFAGINNAKFKQKVVPG
                     DTLELTTEIIKVKGPIGVGKAVARVNGKVACMAELTFAIG"
     gene            complement(4025..>5116)
                     /gene="fabF"
                     /locus_tag="DHV81_04835"
     CDS             complement(4025..>5116)
                     /gene="fabF"
                     /locus_tag="DHV81_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HCI93815.1"
                     /translation="HIAAELKDFNPQDFMDRKAAKRMEPFSQYAVAAAKQAIDDSGLD
                     IEKEDPYMVGCAIGSGIGSLQAMERETQKLYEKGPNRVNPLLVPLMICNMAAGNVSIQ
                     FGLKGKSINDVTACATGTNTIGEAYRSIQYGEADVMVAGGTEGSVCPIGIAGFTALTA
                     LSTVDDPAKCSLPFDKNRSGFVMGEGAGVVILEELEHAKARGAKIYAEVVGYGCSSDA
                     YHITSPQEDGAGAARAMTNAMSDAGVTPADVKYINAHGTGTHHNDLFETRAIKLAFGD
                     EAANLKINSTKSMIGHLLGAAGAVEFITCVKEIQDGFIHKTVGYETPDEEIDLNYCKD
                     SYEEPVEYALSNSLGFGGHNASILLKAYK"
BASE COUNT         1217 a         1363 c          959 g         1499 t
ORIGIN      
        1 gttttatata aaaaaaattc attttattta ttatcttcat ttatattatc ccagttttgg
       61 tactgattct cctcatcatc ggaatatacc accttgaaat aatccgagag ttttgcaagt
      121 gaagttataa gaactttcat ttcgtcatcc gaaagaccgt cctttatatg ctctatcata
      181 tgacggtgaa actgctcatg atgatagtat gcactctttc ccttgccagt gagtgagagc
      241 tttaccacac gcttatcctc ctcactgcgg atacggttca cgtatccctt gcgggtgaga
      301 ccatctattg cctttgtcag tgtgcccaga gtgatagaca taagcttggc tacggtgccg
      361 gagcgctttg gctcatcgac tcctattgcc tctattatat gcatatcgtt gatagaaata
      421 tcagaaaatt cgtctgttat cagacatctg ccctctatct ctgtcaaatc cttgaacaga
      481 cacaccaata attcatttaa agtttcatct gccgtcataa atacctcttt ctattgccac
      541 tctatggcgc aggctcccca tgtaagtcct gctccaaagc ctgataaaat tattttttcc
      601 tcatgcttta tcataccatc tttgcggact ttatcaagca aaatcggcac acttgccgca
      661 gaaatatttc cacaatgctg taaattggtc gggaatttgt ccataggctg cttaagcctc
      721 ttggcaaccg actcaataat acgtatattg gcctgatgca acacataaac atccacatca
      781 tctacgctcc agccgatttc atcaagcagc tcatttatag cctttggcac tgtctttacc
      841 gcaaacttgt atacagcagg accgtccata gtgacatagc caccgagtgc agactgcttt
      901 ccattgtcgg taaacggatt gactacagga cggttgtctc cgtcaagcgc ctcaccgcct
      961 tttccgtccg agccctgtac aaaaccctta aaagcatcgc cctcgtctgt cacaacggct
     1021 gctcctgctc cgtctccgaa aagtacacag gtgcttctgt cattccagtc cattattttt
     1081 gaaagaacct ctgctcccac tacgactgtc ttgtgaaacc tgccggtctt aaaatatgca
     1141 tccgctgtgg caagcccgaa taaaaagcct gaacatgcgg catttatgtc aaatgccact
     1201 gcactgcctg cacctatctc agcctgaatc tgacatgcga gcgatggaac gattttgtct
     1261 gtggatacgc ttgccgctat tatcaagtct atctcacttc cgtcaatccc cgcatcctgc
     1321 agcgcacttt ttactgcatc acaggcaagt gaggtggttg tctcagtcgt tgcaatgtgt
     1381 ctgtttctta ttcctgtacg cgagctgatc cactcgtcag atgtgtccat tatggctgcc
     1441 aggtcatcgt ttgagaccac tctctgtggc aggcagcttc ctgttccaac tatcttcata
     1501 gaatttcctt tccgggacta gaacttgcgg aaacgcttgt atctttcctc caagagctgg
     1561 tcctttgtca atttgctttc tttttccaag aatttcttca tgttgccctt catgtagttt
     1621 ccgattgatg aaagggcatc ctcgtcggct ccgccaaact caggtattat atcatccacg
     1681 acatcaagct cctttaagtc gtgtgctgta atcttcatta cctctgcggc ttcctttgca
     1741 cgctttccgt ccttccagag gatagatgca aagccttctg gcgaaaggat tgagtatgtg
     1801 gcattttcca tcatccacac ttcatttcct acagaaagag ctaaagctcc gccgcttccg
     1861 ccttctccta tcatgagaca aaggattggc accttaatgc cactcatctc ataaaggttt
     1921 cttgcgattg cctcaccctg tccgttttcc tctgcttcca ttcccggata agctcctgat
     1981 gtattgacaa aggttatgat cggacggttg aatttctctg cctgtttcat gaggcgcagt
     2041 gccttgcggt agccctcagg tgatggcata ccatagtttc tctttgcaca atcctcaaga
     2101 tcctttccct tgtggacacc gataactgtg acaggctggc cgtctaagta tgcaattccg
     2161 cctactatag ccgggtcatc gcggaaatat ctgtctccgt gcagctccat aaactcatca
     2221 aagatatagt ccatatagtc aaccgatgca agtctcttaa tctgtcttgc agcgctgacc
     2281 ttatcccata ccttaaatgg cttagccttt gcctcgcgct ccttcataag ctctgtcggc
     2341 tcgtaattgt cgtctgagtg aagcggatca aaattggcat agccagtggt aggtctgtga
     2401 gcacgtaaaa tcttgtaaag agtcttcttt aaatcctcac gctcaactat catatccaca
     2461 aagccgtgct caagctggaa ctctgccctc tggaagccct caggcagctt ctgtccgatg
     2521 gtctgctcga taacacgagg acccgcaaag ccgataagtg cctttggctc tgcaagaatg
     2581 atgtctccaa gcattgcaaa gctggctgtc acaccacctg ttgtaggatc tgtgagtaca
     2641 ggcacatata aaagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaatgat accctcctgc attctggcac
     2761 cgcctgaaca gcagaataat ataaccggaa gtctttcctc tgtggcacgc tcaaatgcgc
     2821 ttgtgatttt ctcaccaacc acatgaccca tgcttcccat cataaatctt gaatcaatta
     2881 caccaagcac tgtctcctcg ccgtaaatct ttgtcttacc gatgacaatg ccctcagata
     2941 gtcctgtttt ctcctgagtc tttgcaattt tctcctcata ctcagggaaa ttgagaggat
     3001 ttcctgttgt aagctctgta aaccatggct caaatgcttc cttgtcggaa accattctga
     3061 ttctgttttt agcacgcaca cggaaataat aattacactc atagcagacg tatttgtgct
     3121 gttttacaac tgatttgagc acagtcttgc cacacttggg acaaacgatt gtctcaggga
     3181 catcctcttt tgcttcctgt actgcaccgg catttttgcc atctgcggct gtttttgcgc
     3241 tctcagcgtt ctttgctaaa gtctcttcag cctctatatt tttatctttc ttcttgaata
     3301 acttcatatt gtctccttat gcaattaacc tattgcaaat gtaagctccg ccatacaggc
     3361 aaccttgccg tttaccctgg caacagcctt gcccacaccg ataggaccct taacctttat
     3421 aatctcagtg gtcagctcca gcgtatctcc cggaaccacc ttctgcttaa acttggcatt
     3481 attgatgcct gcaaaatatg cagtcttgcc cttccactca tcctgacaaa gaatggcaac
     3541 tgcaccggtc tgcgccaaag cctcaataat aagcactccc ggcatcaccg gatttcccgg
     3601 aaaatgtccc ataaaatgcg gctcattcat agtcacattc ttcttagcca cacactttac
     3661 gcccggctcc agttcctcaa ccgtatcaat gagaagaaac ggactgcggt gcggcagaat
     3721 atccataatc tgctgcgtat tcataattga catatacatc tatcctttct caattcgctc
     3781 taaactatct acatgctccg cgctccgcaa cgtggagccg ggaagcacac agtgtgttct
     3841 gaataaaaaa tatgaactgt cacggcgttc catctgctcc aatagcatga agcactaaca
     3901 ccatatcaca gcctgtccgt gcaaaatgcc gtgactgact tcatattttt tatgaggaat
     3961 atactgtgtg ctccaccgga cctcacagcc taaatgcgcg aagccctaaa aaagcagaag
     4021 ctatttattt ataagccttg agcagaatac ttgcattatg tccaccgaat cccagtgaat
     4081 tgctaagtgc atactcaaca ggctcctcat agctgtcctt gcagtaattg aggtcaatct
     4141 cctcatcagg agtctcatag ccaacagtct tatgtataaa tccatcctgg atttccttca
     4201 cacatgttat aaactcaaca gctcccgctg ctccgagcag atgtcctatc atagactttg
     4261 ttgaattgat tttaagattt gcagcctcat caccgaacgc aagcttgatt gctcttgtct
     4321 caaagagatc attgtgatgt gtgcctgttc cgtgagcatt gatgtatttt acatctgccg
     4381 gtgtgacacc tgcatcgctc atggcattgg tcatggctct tgctgcacct gcgccatcct
     4441 cctgcggaga tgtgatatgg tatgcatccg atgagcagcc gtatccgact acctctgcat
     4501 aaatctttgc acctcttgcc tttgcatgct caagctcttc aaggataaca actcctgcgc
     4561 cctcacccat tacaaagccg cttctgttct tgtcaaacgg aagtgagcac tttgccggat
     4621 catctactgt agaaagcgct gtaagtgctg taaatcctgc gattccgata ggacatacac
     4681 tgccctctgt accaccggct accatcacgt cagcctcacc gtactggatg cttctgtatg
     4741 cctcaccgat tgtgtttgtt cctgtagcgc aggctgttac atcattgata ctctttccct
     4801 tgagtccgaa ctggatggaa acatttcctg ctgccatgtt acagatcata agcggaacaa
     4861 gcagaggatt gactctgttt ggtccctttt cataaagctt ttgtgtctcg cgctccatag
     4921 cctgaagact tccgatacct gaaccgatgg cacatccaac catgtatgga tcttcctttt
     4981 cgatatcaag tccgctatca tcaattgcct gctttgctgc agccacagcg tactgtgaga
     5041 atggctccat tctctttgca gcctttctgt ccataaaatc ctgtgggtta aagtccttaa
     5101 gctctgctgc aatgtg
//
LOCUS       DPUF01000136            7460 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7336, whole
            genome shotgun sequence.
ACCESSION   DPUF01000136 DPUF01000000
VERSION     DPUF01000136.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7336"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="DHV81_04840"
     CDS             complement(<1..314)
                     /locus_tag="DHV81_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCI93816.1"
                     /translation="MNTASKTRHVFILFLADIVWGIAFVAQSKGGDAVGAFSFNGIRF
                     LIGALCLLPVIKILDKKELTVNRPESKKQKKELWIAGSCCGIALFFASNLQQLGISMG
                     AES"
     assembly_gap    337..450
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(517..2430)
                     /locus_tag="DHV81_04845"
     CDS             complement(517..2430)
                     /locus_tag="DHV81_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta propeller domain-containing protein"
                     /protein_id="HCI93817.1"
                     /translation="MTKKDLFDGLNYIDDQIIEEAQPTGESAGYSDEIYVKNHETHHG
                     GKEDKNRKKHKKFARTAKALATVAAVLVVAVLGTEVVRLNNRIDELETKTANVISQNN
                     AQNNVYAGIKSGAKLGDYEAASDYGKLYDVAKKAEASIRNTASGDLLESADATGITSD
                     ASSANKQEAAAKTEYSTTNVMTEGVDESDVVKTDGKYIYMVEDGQISITDISNGKPGE
                     ETLFRPDFEVPSDTVEELYISDGKMLIISNHAGDKDETICYSYDIADPTKPVLIGKAR
                     QDGFYNTSRKIGDTVYVFSDTYIKTSDMNKNVALQEDNLEKWVPQVNGKVISYDCFYI
                     GEDTYSGTVISSFDVDKPDKTIDAKCIMNNSGEVYVSGNAMYLYHSDWSASRELTKIS
                     KISFEDGVMKTGETTSVNGYLNDKFAINEQGGYLYVLPTSNTGSQPVNSLHVLDKDMN
                     EVGVINEIARGESIYAARFVGKYVYFITYRQTDPLFVADISNPTAPKLLGELEVSGFS
                     EYLHMWDDTHVLGIGYGDSQQSKIKLTMFDVSDPTKPVEVNQKLIDSSESWSNEFVYN
                     YKAILADPEKNLIGFTANDYYLFSYDSENGFSLLEQQALTYKNTEGYRGIYKDNDFYV
                     AGNGEIKHFKLEE"
     gene            complement(2427..2981)
                     /locus_tag="DHV81_04850"
     CDS             complement(2427..2981)
                     /locus_tag="DHV81_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCI93818.1"
                     /translation="MYTQEQILDMYFERNQQAVSATSQMYGAKLTRFADTMIAHEDAL
                     ECVNDTYIAAWNKIPPERPMRFLSWLYRVCRNIVCDRIDWNNASKRNSNMNCMLDELE
                     ECLADSNAKYEGELSDIGRCLSDFLNGLETEKRVLFVRRYWYGMTIDELADMSGRSKS
                     SVKTTLFRIRAELKKYLDEEGVYL"
     gene            complement(3210..3566)
                     /locus_tag="DHV81_04855"
     CDS             complement(3210..3566)
                     /locus_tag="DHV81_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93819.1"
                     /translation="MKVWKLVSGILSIILFVFVSFQSCAAGVSNALEANGEASGSAGI
                     IVAILMLAGGIVSIATRKSLGKGGNIALIVLFGLAAIIGFAGYGSFSDLAIWAGWCLI
                     NAVLAVVALVMAKKNN"
     gene            complement(3645..4403)
                     /locus_tag="DHV81_04860"
                     /pseudo
     CDS             complement(3645..4403)
                     /locus_tag="DHV81_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743789.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    3735..3820
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4404..4556
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4688..5149)
                     /locus_tag="DHV81_04865"
     CDS             complement(4688..5149)
                     /locus_tag="DHV81_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93820.1"
                     /translation="MKQKNTKELDDILGKTHISDFDKFCVEQKGSMDPEQNAFSEYIK
                     DLLKEKKITQQMVFLKADIPEKYGYKLLSGEKHTRQRDIILRICYAAELTLEQTQRAL
                     RKYEMPQLYAKIPRDAFLMLIFKDRPGGIIEVNELLSKNGMEMLRTSGVQE"
     gene            5227..6381
                     /locus_tag="DHV81_04870"
     CDS             5227..6381
                     /locus_tag="DHV81_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HCI93821.1"
                     /translation="MSDYKIISALNEEHKVYLVQSALSGNIYVQKILDVYNIRVYEYL
                     YRNPVAGIPRLINYYEDGNQLIVIEEYISGTSLQEKIDNSDLSVSDIRSYMIMLCNIL
                     EALHSMTPPIIHRDIKPSNIIITSYNYAMLLDFNAAKQFSGQNESDTVLIGTPGYAAP
                     EQYGFGSSSPKTDIYSLGIVLREMLGSITPVPNIAPILHRLNLIADRCTQMTPAARYQ
                     SVAELKNAVSQSYYPAQHSTSAAGNSSQYAHHNHDNTNNTANADTAKRAIHDSASRFI
                     PPGFRTRTPWKMLLSSVAYLFIIWLCLSLELENTYGAKLWLERIFCLGMFIFAIFCGF
                     NYLNVQNILPLCKSKNRFLHYVGIILLDVAVIFALFVIMFIIESVAFPIK"
     assembly_gap    6537..6589
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6726..>7460)
                     /locus_tag="DHV81_04875"
     CDS             complement(6726..>7460)
                     /locus_tag="DHV81_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent alcohol dehydrogenase"
                     /protein_id="HCI93822.1"
                     /translation="GTEMDPFAVISDMSKNEKWGTGSPYMVPTMSILNPEYTFSVSKK
                     QTAAGTADMMSHTMENYFSMENADCQKFMAEGLLRTMIKNGPAALAQPDNYEARANLM
                     WAGTHAINGVVGDGCSPAWCVHPMEHELSAFYDITHGEGLAILTPAWMEHILNDDTLP
                     MFVEFAKNVWGLSGDDDYALAHAGIDALKKFFFETMGIPANLRAVGITDDRNFEVMAK
                     KACEGSKGSFVPLSKDDIVEIYRAAF"
BASE COUNT         1871 a         1741 c         1211 g         2231 t
ORIGIN      
        1 gattctgcgc ccatgctgat tccaagctgc tgcaggttgc tcgcaaagaa aagtgctatt
       61 ccacagcagc ttccggctat ccaaagctct ttcttttgct ttttgctttc aggtctgttt
      121 accgtcagct ctttcttatc cagtattttt atgaccggca gaaggcagag cgcaccaata
      181 aggaagcgga tgccgttgaa tgaaaatgct ccaactgcat cccctccctt tgactgcgct
      241 acaaaggcta ttccccagac tatatctgcc aaaaacaaaa taaagacatg tcttgtcttt
      301 gaggctgtat tcattataat agttcctccg tgttatnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaaacaccc tctttgggta ttcgccgcct
      481 gtctttatat cttattgcgt aacactatta tttacgctat tcttccagct taaaatgctt
      541 tatctctccg tttcctgcca catagaaatc attatcctta taaatgcctc tgtagccttc
      601 tgtattttta taggtcagtg cctgctgctc caaaagacta aagccgtttt cactgtcata
      661 cgaaaacaga tagtagtcat tcgcagtaaa tcctatcaga tttttctccg ggtcagcaag
      721 aatagccttg tagttgtata caaactcatt actccagctc tcagaactgt cgataagctt
      781 ctggttcacc tcaaccggct ttgtcggatc actcacatca aacatagtaa gctttatctt
      841 actctgctgc gaatcaccat agcctattcc aagtacgtgc gtatcatccc acatatgcag
      901 atattcagaa aatccactca cctcaagctc tcccaaaagc tttggtgctg tcggattaga
      961 tatgtctgct acaaaaagag gatccgtctg cctgtatgtg atgaaatata cgtattttcc
     1021 aacaaagcgg gctgcgtata tactctcccc gcgtgcaatc tcgtttatca caccgacctc
     1081 attcatatcc ttatccagca catgaagcga attgacaggc tgactgccgg tatttgaggt
     1141 tggaagcaca tacagatacc ctccctgctc atttattgca aatttatcgt taagataacc
     1201 attcactgaa gtcgtctccc cggtcttcat cactccatcc tcaaacgaaa ttttgctgat
     1261 tttggtaagc tcacggcttg cactccaatc agaatgatac agatacatag cattgccaga
     1321 aacgtacacc tcgccgctat tattcataat acattttgca tctattgtct tatcaggttt
     1381 atccacatca aagctcgaaa taactgtgcc tgagtatgta tcctctccta tatagaagca
     1441 gtcatagctt atcactttgc catttacctg aggcacccat ttctccagat tatcctcctg
     1501 cagcgccaca tttttattca tatcggaggt ctttatatat gtgtccgaaa aaacatatac
     1561 cgtatcgccg attttgcgcg atgtattgta aaatccatcc tgacgtgctt tgcctataag
     1621 caccggtttc gtcgggtcag ctatatcata gctatagcat atagtctcat ccttatcgcc
     1681 cgcatggttt gatatgataa gcatttttcc gtcactgata taaagctcct ctacagtatc
     1741 tgacggtacc tcaaaatcag gacgaaacag cgtctcttcc cccggctttc catttgaaat
     1801 atccgtgatg gatatctgac catcctcgac catgtaaatg tactttccgt ccgtctttac
     1861 cacatcactc tcatcaacgc cctctgtcat cacgttggtg gttgaatact ctgttttggc
     1921 tgccgcctcc tgcttatttg cagacgatgc atcagacgta attcctgttg catccgcact
     1981 ctccagcaag tctccagatg cagtattcct tatggaagcc tcagcttttt ttgccacatc
     2041 atacaatttc ccgtaatcag aggccgcttc ataatcccca agctttgcac cgctctttat
     2101 ccctgcatag acattattct gtgcattatt ctgtgaaatc acatttgcag tttttgtttc
     2161 cagctcatct atacggttat taagccgcac aacctccgtg cctaagactg caacaacaag
     2221 gactgccgcc actgtggcaa gcgcttttgc tgtccgtgca aattttttgt gctttttcct
     2281 gtttttgtct tcttttccgc cgtgatgggt ttcatgattt tttacataaa tttcatcact
     2341 gtacccggcc gattctccgg tcggctgcgc ttcttcaatt atttggtcat cgatgtaatt
     2401 taaaccatca aataaatcct ttttcgtcat agatatacgc cctcctcatc cagatatttt
     2461 ttaagctctg cccttatccg aaacagtgtg gtctttacgc tgcttttgct cctgccgctc
     2521 atatctgcca gctcatcaat cgtcatgcca taccagtagc gtctcacgaa aagcactctc
     2581 ttttccgtct ccagtccgtt cagaaaatcc gataaacacc ttccaatgtc cgacagctcg
     2641 ccctcgtatt ttgcattgct gtctgcaagg cactcctcaa gctcatcaag catacagttc
     2701 atgttggaat ttctctttga agcattattc cagtcaatcc tgtcgcagac gatattgcgg
     2761 cataccctgt aaagccagga aaggaaacgc atagggcgct ccggcggtat cttgttccat
     2821 gctgctatgt atgtgtcatt tacgcattcc agcgcatcct catgtgctat catcgtgtct
     2881 gcaaaccggg taagcttcgc tccgtacatc tgcgaggtcg cactgacagc ctgctggttg
     2941 cgctcaaaat acatatcaag tatctgttcc tgtgtgtaca ttttccacaa acctcccttc
     3001 tatatattat gtaggatttt gtttggtggg ggttacattt catcattaat tctttaaaat
     3061 ttcccttatg taaactttaa gcctagcctc taggaaataa agcttatagt gctgaataat
     3121 tgaattgtat catatttagt cttagtttaa atatcaaaaa aggaagattc tccttagaat
     3181 cttcctcata tgaattcaaa tagtaatatt taattgttct tctttgccat aaccagtgca
     3241 accaccgcaa gaactgcatt tatcagacac cagcctgccc atatagcaag atccgaaaaa
     3301 cttccataac ccgcaaatcc gataattgct gccaatccaa atagtactat gagtgcgata
     3361 ttcccaccct tgcctaatga ttttctcgta gcgattgaaa caattccacc tgcaagcata
     3421 agaattgcca caattattcc tgcacttccc gatgcctcac cattggcctc caatgcatta
     3481 gatactcctg cagcgcatga ctgaaatgat acaaaaacaa ataaaattat tgataaaata
     3541 cctgaaacta atttccaaac cttcatgatg aatttctcct atctattaaa tattgcgtag
     3601 ctctactgtt taataatcat actttcatat gatgtttaaa ttatttactg tgccgtaaat
     3661 gttatcgcat ctgaatccgc tattgtatcg tagctctcag cattgtatat atggaattta
     3721 agctccacct gatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn actgtggttg aaaacagcgg
     3841 tgtcatcata tatccattga tagacatatt gtccacgttt attccgacat tctgacctga
     3901 ggtgttttcg atgtaaagta aaattgctgt tccccagaag gaattttcat caactgtttt
     3961 tccaacaatc cttacaccat ttgcattgta aagctcctgt ccctcatcgt ttggtgtcgt
     4021 atccatgttt gcaaattcag aggtctcaag ctttgaatat acattcttga aaagattatc
     4081 ccaattggaa tcgtatgcgt ggaaatacat ctcaatttgt cctactgtat ctattccggc
     4141 agctttaagc tctgttgatg ataaatacat aacctcattc gacttctttc ctgccgcaac
     4201 atccgcgctg aacagatctg ttatcatata atcgttcacg ataagtgcat cacagcctat
     4261 tgtatagtcc ttggttgaat tattttcaac cataagctta attccatctc cccatatgct
     4321 gtccgtcaca tattcagtcg cagttatttt gatgccatcc tggtctacaa gcacctgctc
     4381 attgatgctc tcatctgctg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctca
     4561 gtacaagagc cagtaaatta gttctttttt tcatggttaa gtcctcctct gtaaattgta
     4621 ggactattat accatcacag tatgaatacc gttctccccg cagcggtggt aaaattatat
     4681 tctaaaccta ctcctgcaca ccacttgtcc tgagcatttc cataccattt ttgctgagta
     4741 attcattcac ctctatgatt ccacccggac gatccttaaa aataagcata agaaaagcat
     4801 ctctcgggat ttttgcatac agctgtggca tctcgtattt tctcaaggct ctctgtgtct
     4861 gctctaatgt aagctctgca gcataacata tcctcaggat aatatccctc tgcctggtat
     4921 gtttttcgcc tgacagaagc ttgtagccgt atttttccgg aatatctgcc ttcaaaaaca
     4981 ccatctgctg tgttattttc ttttccttaa gcaaatcctt tatgtactct gaaaatgcat
     5041 tttgctccgg atccatgctg cctttctgct caacacaaaa tttatcaaaa tcagatatat
     5101 gagtttttcc aaggatatca tccaattctt ttgtattttt ttgcttcata aatttctcct
     5161 atctgttata cttgtacttg taactattcc tacctcatta gcgatacctg aaaggataca
     5221 tgcaccatga gcgattacaa aataatctca gccttaaatg aagagcacaa ggtttatctg
     5281 gttcaatctg cgctttccgg caatatttat gtccagaaaa tccttgatgt atacaatatc
     5341 cgcgtgtacg aataccttta tcgcaatcct gtggccggta ttccgcgcct tatcaattac
     5401 tatgaagatg gcaaccagct tatcgtaatc gaagaatata taagcggaac ctccctgcag
     5461 gaaaaaatag acaactctga ccttagtgtt tccgatatca gaagctacat gattatgctc
     5521 tgcaatatac ttgaagctct gcactcgatg actccgccta taatccatcg cgatataaag
     5581 ccctcaaata tcataatcac cagttataac tatgccatgc ttttagattt caatgccgca
     5641 aaacaatttt caggacaaaa cgaatctgat accgtgctga ttggcacccc cggatatgca
     5701 gctcccgagc agtatggctt tggctcttca tctccgaaaa cagacatata ttctctcggc
     5761 atagtactgc gcgaaatgct tggcagcatc actccggttc ccaacattgc tccaattctg
     5821 caccggctta acctgattgc agataggtgt actcagatga caccagccgc aagataccag
     5881 tctgtggcag agttaaaaaa tgccgtatcg cagtcatatt atcctgcgca gcacagcact
     5941 tctgctgccg gtaattcaag tcaatatgca caccataacc atgataatac aaacaataca
     6001 gccaatgccg atactgctaa acgagctatc cacgacagtg cttcccgttt catcccaccc
     6061 ggatttcgca cgagaactcc atggaaaatg ctgctatcct cagtcgcata tctgttcata
     6121 atatggcttt gcctgtcatt ggaattagaa aatacttatg gtgcaaagct gtggcttgaa
     6181 agaatcttct gtcttggtat gttcatattt gcgattttct gtggctttaa ctatctcaat
     6241 gtacagaata tactgccact ttgcaagagc aaaaaccgct ttctgcacta tgtcggcata
     6301 atactgctag atgtggcagt gatttttgct ctatttgtga ttatgttcat tatagagtct
     6361 gttgcattcc ctataaagtg attattttat aactattcag tacagaaata tgcggtaccg
     6421 catatttcgt aagaaaacgt atattatgca ggagcaagcg cccatcagcg cagctgattc
     6481 taatgcgctt gcggctcgca actgtgaagg tactgaacag ttacgatttt ttatatnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tagcacataa
     6601 aaataagcca tgttctcacc gctgtggtgg taacatggct tattaaagat aaatatattt
     6661 tgttctcata catcaaagag aatttcagtc aaattattaa tcaaggtatt tgcaggaacg
     6721 attgattaga atgccgctct gtaaatctcc acaatatcgt ccttgctaag cggtacaaac
     6781 gatccctttg aaccctcgca ggccttcttt gccatcacct caaaattcct gtcgtctgtg
     6841 attcccacag ctcttagatt tgcaggaatg cccattgtct caaagaagaa tttcttcagt
     6901 gcatcaatgc ctgcgtgtgc aagtgcatag tcatcatcac cggaaagtcc ccagacattt
     6961 tttgcaaact ccacaaacat cggaagtgta tcgtcattta gtatgtgctc catccacgcc
     7021 ggtgtgagta ttgcaagtcc ctctccgtga gtaatatcat agaaagcgct cagctcatgt
     7081 tccataggat gtacgcacca tgccggtgaa catccatcgc ccacaacacc attgattgca
     7141 tgtgtgcctg cccacatcag gttggctctg gcctcgtagt tgtcaggctg tgcgagggca
     7201 gccggtccat ttttaatcat tgtacgtaac aatccctctg ccataaattt ctggcagtca
     7261 gcattctcca tagagaaata attctccatt gtatggctca tcatatctgc ggttcctgct
     7321 gcagtctgtt tttttgatac tgaaaaagta tattcaggat ttaatattga catggttggc
     7381 accatatatg gtgatcctgt tccccacttt tcatttttag acatatcaga gataaccgca
     7441 aaaggatcca tctctgttcc
//
LOCUS       DPUF01000137            2974 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_46743, whole
            genome shotgun sequence.
ACCESSION   DPUF01000137 DPUF01000000
VERSION     DPUF01000137.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_46743"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(51..2582)
                     /locus_tag="DHV81_04880"
     CDS             complement(51..2582)
                     /locus_tag="DHV81_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93823.1"
                     /translation="MNFVKKHPLLTAFLIPFFICIIICIGNGVYPFGDYCLLHMDLYH
                     QYLPFFNELWEKLRNGASLMYTWNIGLGSDFVSVFAYYLASPLNWLVVLFPKSHIIEF
                     IELLIIFKISLSGATFFYFLKEHFVLLGKDNRYHNSTFVPAFVFSTAYALSGFVAAYS
                     WDVMWMDVVAVAPLAIVGLEKLVRDKKPVFYYVSLALCILSNYYLSIMLCIFLVFWFA
                     WLFFTQNGGKMAAIVRFAVYSLLAGGTAMVLIIPELIILSYSGSSGIEFPKQIEWYFN
                     LLSEVGRMCTTASVYEGAENWPNLYAGAFSVMLLILFVLNKRINWKKKIAPVLFVLFF
                     MASFANKQLDFIWHGLHFPDSLPGRQSYLYAFLVLTIGYATVRKWRGIRLWHIGVAVV
                     FASALMAVSTMFADEDVTEYYAVIISILFVALYGIMTVLLKLAARKNRELLMVITCGI
                     AIVELAVNMAVTGLGCTGRSSYNANVDDMQKALELAKEDAADNDVPFYRVEDTGRLTK
                     NDGTRYGYASGTQFSSLMNINVSHFYQALYMEGGKNFYCYNGATPVTSAMLSVRYMVT
                     KSIQPQNELTTLVGKCGNHYLYRNNYTLPLGFMMDEGVIDAWKPSSSSKIYSINSLGR
                     LLGAADDTLTLTECTQDENPGTTTLTFDHDGYYYAAYDSCSTDSLTFSHGEYETTYSK
                     TTHRYLFDLGYVKAGDTVSVTNTDADAVRFNVYELSIAAVESAYNTLNEQTLSVDDFS
                     DTHISGHIDVKQAGQLVLSIPSEKGWTMKVDGKDAEYEDFSDTFVSIHLNEGEHEIEL
                     YYETPGLKAGAAISAGCVGVFLLLLLLRQIVKRRSRLKFKANSDR"
     gene            complement(2582..>2974)
                     /locus_tag="DHV81_04885"
     CDS             complement(2582..>2974)
                     /locus_tag="DHV81_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCI93824.1"
                     /translation="SSVLAANGMDYNNLSNEELKLRYQVPYVIWANYDIDEAAGKDTS
                     VNYLAANVLKAAGVPTNDYQSFLLKLQEEYPIISAVRTDKTADKTLDKASNKSDKATG
                     SKNKQADSDMLNDYKLLQYYRLFDWEDK"
BASE COUNT          875 a          747 c          541 g          811 t
ORIGIN      
        1 ttctgtgttt cgcataaatg aactgtatgc gttacaggtc attgtaagca ttatctgtca
       61 ctgttcgctt taaactttaa acggctgcgt ctcttaacta tttgacgcaa caataaaagc
      121 agtagaaaga cgcccacaca gcctgcgctt atagctgcac ctgccttaag ccccggtgtc
      181 tcataataaa gctctatctc atgctctccc tcattcagat gaatgctcac aaaagtatct
      241 gaaaaatcct catactctgc atccttccca tccaccttca tagtccatcc cttttcactc
      301 ggaatagaaa gcacaagctg tcctgcctgc ttcacatcaa tgtgtccgct tatgtgtgta
      361 tctgaaaaat catccaccga aagtgtctgc tcattgagtg tattgtaagc actctcaact
      421 gcagcaatgg aaagctcgta tacattaaat cgtacagcat cggcatcagt gtttgtgacg
      481 gaaaccgtgt cccccgcctt cacatatccc aggtcaaaca gatacctgtg agtggtcttc
      541 gagtatgtgg tctcatactc tccgtggctg aatgtcagac tgtcagtgct gcacgaatcg
      601 tatgccgcat aataatatcc gtcatggtca aaggtgagag tcgtagttcc cggattttcg
      661 tcctgggtgc actctgtcag ggtcagcgta tcatctgctg cgccaaggag cctgccaagg
      721 gaattaatcg aatagatttt cgatgacgat gatggcttcc aagcatcaat cactccctca
      781 tccatcataa aaccaagtgg aagcgtgtag ttgttccgat acagatagtg atttccgcat
      841 tttcccacaa gtgtagtcag ctcattctgt ggctgtattg attttgtaac catatatctg
      901 accgaaagca tggctgacgt gacaggtgtg gccccgttat aacagtaaaa attcttgcct
      961 ccctccatat aaagagcctg ataaaaatgg ctcacattta tattcatcag ggaagaaaac
     1021 tgtgttccag aagcatagcc gtatcgtgtg ccatcatttt tagttagtcg gcctgtatcc
     1081 tcaaccctgt aaaatggtac atcgttgtca gctgcatcct cttttgccag ctccagtgcc
     1141 ttttgcatat cgtccacatt ggcattgtag ctgcttctgc cggtacatcc aagtcctgtc
     1201 acagccatat tgacagccag ctccacaatc gcaataccgc atgttatgac catcaaaagc
     1261 tccctgtttt tccttgctgc aagctttaaa agtactgtca taatgccgta cagcgcaaca
     1321 aacaatatgc ttatgataac tgcataatac tctgtcacat cctcatcggc aaacatcgtg
     1381 cttactgcca taagcgccga tgcaaatacc actgccacac caatatgcca cagtcttatg
     1441 ccacgccact ttcttactgt agcatagcct atggtcaata caagaaatgc atacagatac
     1501 gactgtctgc caggcagtga atctggaaaa tgcagtccgt gccatataaa gtcaagctgt
     1561 ttattggcaa agcttgccat gaaaaacaac acaaacaata cgggagctat ttttttcttc
     1621 caattaatcc ttttattgag cacaaacagt atcaaaagca tcacactaaa ggctccggca
     1681 tacagattcg gccagttctc tgcaccctca tagacgcttg ctgtcgtgca catcctgccg
     1741 acctctgata agagattaaa ataccactct atctgctttg gaaactcgat gcccgacgaa
     1801 ccgctgtagc ttaaaatgat aagctccggg atgattagca ccatcgcagt gcctccagcc
     1861 aaaagcgagt aaacggcaaa tctcacaatt gcagccattt taccgccatt ttgcgtaaag
     1921 aaaagccatg caaaccagaa taccaaaaat atgcaaagca tgatgctcag ataatagttt
     1981 gacaatatac aaagtgccag tgatacatag taaaacaccg gctttttgtc ccgcacaagc
     2041 ttctcaagtc ctactatcgc aagcggtgca accgccacaa catccatcca catgacatcc
     2101 cagctatacg cagctacaaa gccggaaagt gcatatgccg tagaaaaaac aaatgccggc
     2161 acaaatgtac tattatgata cctgttatcc tttccaagaa gcacaaaatg ctccttcaaa
     2221 aaatagaaaa atgtcgctcc gctaagcgaa atcttaaaaa tgataagcag ctcaataaac
     2281 tcaataatat ggctctttgg aaaaagcacc acaagccagt ttaacggact cgccagataa
     2341 taggcaaata ccgatacaaa atcactgcca agcccaatat tccatgtata catgaggctc
     2401 gcaccatttc ttagcttctc ccacagctca ttaaagaaag gcagatactg gtgatacaaa
     2461 tccatatgca aaaggcagta atcgccaaat ggatatacac catttcctat acagatgatt
     2521 atacatatga aaaacggaat taaaaacgct gtcaaaagtg gatgcttttt aacaaaattc
     2581 atcacttatc ctcccagtca aacagtctgt aatattgcaa aagcttataa tcgtttagca
     2641 tatccgaatc agcctgctta tttttgctgc cggttgcctt gtctgattta tttgatgctt
     2701 tatctaatgt cttatctgca gttttatctg tgcgtaccgc tgatataatt ggatactcct
     2761 cctgcagctt tagcagaaag ctctgatagt catttgtcgg cacacctgcc gctttaagta
     2821 cgtttgcagc cagataatta acgctcgtgt cttttcctgc cgcctcatca atatcataat
     2881 ttgcccagat aacatacggc acctgatagc gcagcttaag ctcttcattt gataaattat
     2941 tatagtccat gccgtttgct gcaagaacag agct
//
LOCUS       DPUF01000138            5902 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_5050, whole
            genome shotgun sequence.
ACCESSION   DPUF01000138 DPUF01000000
VERSION     DPUF01000138.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_5050"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="DHV81_04890"
     CDS             complement(<1..881)
                     /locus_tag="DHV81_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI93825.1"
                     /translation="MKKKLVSALLCATMATSLLAGCGSGNASDTGSADKKGDAKTEVT
                     NDGKILNIYCWNEEFQSRITDHYPDYKKVDATHGKIGDVDVVWNITPNENNAYQNNLD
                     ETLLKQADASADDKIDLFLVEADYAPKYVDSDYTMPIKDLGITDSDISKQYKYTQDVV
                     TDSDGNLKGLSWQGCPGVLFYNRAAAKEVLGTDDPDEVQKSVADWDTFNATAEKMKAA
                     GYKMTSTANDTYRVYSNNVSKKWVSDDKKIQIDDNIMKWVDDSKKLVDAGETGTADLW
                     SDDWKKGFYPEGKVFSY"
     assembly_gap    1054..1167
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1168..1979)
                     /locus_tag="DHV81_04895"
     CDS             complement(<1168..1979)
                     /locus_tag="DHV81_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HCI93826.1"
                     /translation="MVSLKDIAQRCGVSTATVSKALHDQKDIGAETKNRIKKIAKEMG
                     YLPNAAARVLKTNESNNIGVLYFDEAAMGLTHEYFAGVLNGIKAQAEMLGYDITFINT
                     SPVGKKLSYLEHCKYRNFDGVVIVCARFQDNEVQELMSSDIPVVTIDYVHYSCSAVSS
                     NNVKCMGELLDYIISMGHKKIAYIHGQDFSSVTRDRLAAYYRALEKHGIDIPEEYVRE
                     ARYLETEDVAKQTEYLLDLPDKPTCIIYPDDTAAIGGINVIRSRGLRIPEDIS"
     gene            2363..4509
                     /locus_tag="DHV81_04900"
                     /pseudo
     CDS             2363..4509
                     /locus_tag="DHV81_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568904.1"
                     /note="too many ambiguous residues; incomplete; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     assembly_gap    3259..3361
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4510..4653
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4654..4866
                     /locus_tag="DHV81_04905"
     CDS             <4654..4866
                     /locus_tag="DHV81_04905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93827.1"
                     /translation="IIDPCIPDTMDGFTAKRKFRGNTYHITVRNPAHVQKGVTKLIVD
                     GATIDGNMIPAINGTGHDVTVEVTMG"
     gene            complement(5005..5345)
                     /locus_tag="DHV81_04910"
                     /pseudo
     CDS             complement(5005..5345)
                     /locus_tag="DHV81_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516917.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3837 domain-containing protein"
     assembly_gap    5062..5129
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5465..5902)
                     /locus_tag="DHV81_04915"
     CDS             complement(5465..5902)
                     /locus_tag="DHV81_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HCI93828.1"
                     /translation="MAFLAGAGVSAGVFAFVLVIGVVPRILRCSRIENIILIENVMIC
                     GVVAGNISSVLDTAYNLGFANMPKVPVSNICGNIIIAIYGLCVGIFVGCIAVALAEIL
                     HTFPIIYKRMKLRAGLGIIVISMAIGKALGALYYFLTGFVATS"
BASE COUNT         1452 a         1285 c         1078 g         1658 t
ORIGIN      
        1 aagtatgaga atacttttcc ttccggatag aagcccttct tccagtcatc tgaccaaaga
       61 tcagctgttc ctgtctcgcc tgcgtctaca agcttcttag aatcatcaac ccacttcatg
      121 atgttgtcat caatctggat tttcttgtca tcgcttaccc atttcttaga aacgttgtta
      181 gagtatacac ggtatgtatc gtttgctgta gatgtcatct tgtagcctgc tgctttcatc
      241 ttctcagctg tagcattgaa ggtatcccag tcagctactg acttctgaac ctcatccgga
      301 tcatctgttc caagtacctc ttttgctgct gctctgttat agaagagaac acccgggcag
      361 ccctgccatg aaagaccctt taagttgcca tctgagtcag taacgacatc ctgtgtgtac
      421 ttgtactgct tagagatatc gctgtctgtg attcctaaat ccttgattgg cattgtgtaa
      481 tcagaatcta catatttagg tgcgtagtca gcctctacca ggaagagatc aatcttgtca
      541 tctgctgatg catcagcctg cttcaaaagt gtctcatcaa ggttgttctg gtatgcattg
      601 ttctcattag gtgtaatgtt ccatactaca tcaacatctc cgattttgcc atgtgttgca
      661 tccaccttct tgtaatcagg atagtggtct gtgatacggc tctggaattc ctcattccag
      721 caatagatat tgagaatctt gccatcgttt gtcacttcag tttttgcatc gcctttcttg
      781 tcagcgcttc ctgtgtcaga tgcatttccg ctgccacagc ctgccagtaa tgatgttgcc
      841 attgttgcgc aaagtaatgc gcttacgagt ttctttttca tagaaaaatc ctccttctcg
      901 cttcaggttt aaacctgtta gtaaatgtta gtaaaaaagt agtaaaatat attaaattct
      961 gcctacggat tccccctcca gcaaaattcc ttcaacaaca gctctctcca caagtgctgt
     1021 tttaggcttt ttaatgatgc ttatcaacct gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnngat atatcctccg gaatcctaag ccctctgctt
     1201 cttataacat tgatgcctcc gattgccgcc gtgtcatccg gatagatgat acatgtaggt
     1261 ttgtcaggta aatcaagtaa atattctgtc tgctttgcaa catcctctgt ttccagatac
     1321 cttgcttccc tgacatactc ctcaggaata tctattccat gcttctcaag tgccctgtaa
     1381 tatgctgcca gcctgtcacg tgtaacactc gaaaaatcct gtccatgtat gtaagcgatt
     1441 ttcttatgtc ccatgcttat tatgtaatca agcagctcgc ccatacactt tacattgttg
     1501 gaacttactg ccgaacagct atagtgtacg tagtcaattg tgaccaccgg tatgtcgctg
     1561 ctcatcagtt cctgcacctc attgtcctga aaccttgcac atacgattac gactccatcg
     1621 aaatttctgt acttacagtg ctccaggtat gataattttt ttccgaccgg acttgtattg
     1681 ataaatgtaa tatcatatcc aagcatctca gcctgtgcct tgattccgtt tagcactccg
     1741 gcaaaatact catgcgtaag ccccatcgcc gcctcatcga aatacagtac tccgatgttg
     1801 ttcgactcgt ttgtcttaag aactctggcc gcagcattag gaagatatcc catttcctta
     1861 gctatcttct ttattctgtt tttggtctca gcgccaatat ccttctggtc atgtaatgcc
     1921 ttgcttactg tcgctgtcga aacaccacat ctttgtgcta tgtctttcag tgataccata
     1981 tcgtttcctc atttcgaatt ggtttcttaa tcgttttcgt atcttcacta taccacaatt
     2041 cattattatt gcaaccccta atttacattt tcaacatttt taacactttt tattatcttt
     2101 tattatgcat tttgtatagt gttatgttat gtatttaaaa cgattacgcc tttgatctta
     2161 attattatac aattataatt acttaagtga tattatctca tcagcatttt taactataat
     2221 atgcagtttt ctttgtatat atgtcataaa agcattattt tttattgttt tatgcttgat
     2281 ttttagatat tcgcattata tacttctata tataaacagc ttaaacgttt gcgtatattt
     2341 aataaaaaag gagattttat atatgaaatt tggacacttc gatgatcaaa acaaagagta
     2401 cgtcatcacc tctccaagga ctccgttacc atggatcaac tacctcggat gtgagaattt
     2461 cttttctctg gtatcaaata cctgcggtgg ctacagcttc tataaggatg caaagcttct
     2521 gcgtctcacc agataccgct acaacaacgt gccatacgac agcaacggac actactacta
     2581 tataaaggat ggcgacacaa tctggaatcc gggctggatg ccttctaaga ccgagcttga
     2641 ctcatacgaa tgccgtcatg gcatgggcta ttctgttttc acaggagtaa aaaacggctt
     2701 aatggctcag cttaccgact ttgttcctat gggctctacc tgcgagatca ataagctcac
     2761 attaaaaaac acatctgata agaaaaaaga cttttcggtt ttctcatatg tagagttctg
     2821 tctgtggaat gccatggatg acatgaccaa cttccagcgt aacttctcta ccggagaggt
     2881 tgagattcac gccggtggct ctcagctctt ccacaagaca gaatacagag aacgccgcaa
     2941 tcactatgct gtatatgctg taaatgcctc tgttgacgga tttgacacag acagggattc
     3001 attcttaggt gcctacggcg aaaacagcgc gccttccgtt gtagtaaatg atcagtctaa
     3061 gaactctgtt gcaagcggct gggctcctgt cggctctcat cacctgaagg tagctcttga
     3121 gccgggcgaa gaaaaaacat atatatttgt acttggatat gtagaaaacc ctgtgaacga
     3181 aaaatgggtt ggacgcgctg aggatggaat cataaaccgc gctcctgccg atgcactcct
     3241 tgcaagattt gacaccacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 ngaggaaaag cttgaccgca tggtaaatgt atggcatcag tatcagtgca tggtgacatt
     3421 taacatgagc cgctctgctt cttattttga atcaggcatc ggacgaggca tgggctttag
     3481 ggattcctgt caggatctgc ttggcttcgt acatctgatt cctgacagag caagagaaag
     3541 aatactcgac attgcagcca cacagtttga ggacggctct gcttaccacc agtaccagcc
     3601 tctcaccaaa aagggcaatg ccgacatagg ctcaggcttc aacgatgacc ctctgtggct
     3661 tatcgctgct gccgctgctt acatcaagga gacaggcgac tactctattc ttgacgagtc
     3721 aacaccatat gacagcgatc cttcaaaggc aactgacttt atggagcatc tgcgccgctc
     3781 cttcaactat acaatcaatc atctcggacc tcatggtctg ccacagattg gacgtgctga
     3841 ctggaacgat tgtctgaact taaactgctt ctccgaggag cctggtgagt ccttccagac
     3901 ctttggacca tctgaaggac ctaatgccga atctgttttt atcgcaggca tgtttgtaaa
     3961 atatggaaaa gactatgtaa aaatctgcag acacaaggga ctttgcgacg aggctgatac
     4021 agccaaaaag gctattgaac agatggaaaa gaccgtcatg gatgccggct gggatggcga
     4081 gtggtatctt cgtgcttacg accactacaa gcacaagatt ggttccaagg aatgtgagga
     4141 tggaaaaatc tttattgagc cacagggctt ttgtgttatc gctgaaatcg gaaaggatga
     4201 agggttctgc cttaaggcaa tgcagtctgt tgaaaaatat cttgatacta aatatggcat
     4261 cgtgctcttg cagccgcctt accacagata tcatgtggag cttggtgaaa tctcttctta
     4321 tccaccggga tacaaggaga atgccggcat cttctgccac aacaatcctt ggatttctat
     4381 agctgagact gttatcggcc gtggaaacag agcatggcag gtatacaccc ggacctgtcc
     4441 tgcatacatt gaggatatca gcgaaattca ccgcaccgag ccttatgtat acagccagat
     4501 gattgccggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcatag atccttgtat tcctgacact
     4681 atggacggct ttacagctaa gcgtaagttc cgtggcaata cttaccacat tacggtcaga
     4741 aatcctgccc acgtacaaaa gggtgtgaca aagctcatcg tggatggcgc tactattgat
     4801 ggcaatatga ttccggctat taacggaacc ggacacgatg ttacagtcga ggttacaatg
     4861 ggttagagat accagacagg cctctatact gcttcaaata tgacccggaa tacgaaaatg
     4921 ccgccgcaga gcaagctcta cggcggcatt tagactgctg gtgatgttct tcccatgtct
     4981 ttatttcttc tctaaaggat gctgttatgt aattgagacc tgccacatat gctcctcggt
     5041 ctctcccatg tgaaacagct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agatacttct cctgctcatc tgccggcgat
     5161 accttatcaa tgtgtttttg caattcctca agaagctgct ttggctccat gtcggcatcg
     5221 actgcgattc caaagcactt cttcgcaaga cctgcggtat aatacagctc gtagttactt
     5281 acaagcactg atttttggcg taagcatttg agtatgatgc tttgtctggc tattgatgta
     5341 atcatattat ttcctttccg agttagctgg aatacctcta agtccaagtt atttccatta
     5401 taccctattt gctttttgtt tcaagagtat aacatgatat agctaaagat aacttaagag
     5461 agtcctatga agtggcaaca aatcccgtca ggaaataata aagtgctccc agtgcctttc
     5521 caatagccat agaaatcact ataatcccaa gaccggcgcg aagcttcatg cgcttatata
     5581 taatagggaa tgtgtgcagt atttctgcga gtgcaactgc aatacagccg acaaatatgc
     5641 cgacacacaa tccatatatt gcaataatta tattcccaca gatattgctt acaggtactt
     5701 tgggcatatt tgcaaagccc agattatatg ccgtatcaag cactgatgat atattacctg
     5761 ccaccacacc acatatcatg acattttcta taagtattat attttctatc ctgctgcacc
     5821 gaagtatgcg cggcaccact cctatcacga gcacgaatgc aaatacaccg gctgacactc
     5881 ctgcccctgc taaaaacgct at
//
LOCUS       DPUF01000139            7064 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_21138, whole
            genome shotgun sequence.
ACCESSION   DPUF01000139 DPUF01000000
VERSION     DPUF01000139.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_21138"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2173)
                     /locus_tag="DHV81_04920"
     CDS             complement(<1..2173)
                     /locus_tag="DHV81_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5057 domain-containing protein"
                     /protein_id="HCI93829.1"
                     /translation="MSKLRKKIVVPISLTLTGCLVVAAFVLNDSLTKVDANVAFNGIS
                     DIVSSHGKDKPFNIVEVVPDKKMASIGYLIDGQEPDDWFGTLSKMSDKEGDGVQKRAD
                     YMSGLKTKLAPITTEKKDNTKPLYYEPYEESYVSLDDDWSELALVDMDRINKGTTGYK
                     MTEQAEGDYKFNTDYQLAVNEDGLPTGHYRQNVDHYVFMQGEDETEDGSESVDTGKRG
                     YYSVAFTAVKLPDGMTNTQYLVETDNSQKADGNTDSGTDGNIDSSTDNTTDTGTGDDT
                     VEKHVVYSIKSAYAIVSAEGMQNIAKYDPKAYIYRTDKSDTTSPYEFVARADRMASLP
                     DGSKPDYEKYTYFTVEMEYVPADKITDDETYYEVDTEVPIEFNYDETGEYGAVLDSQN
                     PYEKIDDGTPDNQPAGKTGADDISDDASIADSATENNDGNTDRVILTDTDGYFDIVKD
                     SQTYTYVGKGKGDYIMVADKNGSLDYPVNTTHIYYKGGFKNNNWFRNGVFNQEGKKGD
                     DDKTANMTFNVKTVTPKELEQIDVSTIDLLYLSGSKSVLSNDLVNDAATYKTDNDISW
                     DKVKQIVQRVHQSGIMMPVIVDNGIVWTASSADYSSNIKKLAGLLSCNNFSSLNFTKD
                     SADNFINWNNDIKYYKVDSKTHTKGFVIGNEYVIPRNYNPSTDVPFVLRDDFASAFIA
                     NEDETQFVEAAKNENFDEIAEYINSENTSRKKENDTLGKDEY"
     gene            complement(2209..3684)
                     /locus_tag="DHV81_04925"
     CDS             complement(2209..3684)
                     /locus_tag="DHV81_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCI93830.1"
                     /translation="MKLLRRMNKSNNRKNSGFTLVELVIVMAIMGILGLAVAGFIGTS
                     TKQYKYASKDVDLQYEAQLTMNQIGDLIIDAQKGVKYEPATVAAPVEVASAAPEAGYV
                     ATASAEEASSEEASSEETSSDSKLIIYNKDCTYNIIYRPSEHKIYLRKDTLDPATGAV
                     KADGQGKESLMAENVTGFTPDLSEAESKNSVGLVVDFKAGDKKYTSKQNFTMRNKVPT
                     GADVEYVEPAEPKGIRIYYKGKDVTNGTVYYEMKKNQNKLEFTDKILGGKYDSKNVTW
                     DHSGTKNGGNFNANGDVYNVELMDNETTDQFVITVTSKDDTSLTAKVTVNVAHPINIL
                     HCDADKHFNLGQTHQLFIDEWELKNSSLNGKYQVKDFVWHMEVESVSSTGATEHKGDI
                     SFRMGDDGKLIYGPEGNGEPDGIFKFSSNEIAGETGAQGKPDDPKYKNYLNYYILGYN
                     KEMHITLGNDFLTGEKLNISVWLGLEDMPEFKSNTITFYTY"
     gene            complement(3769..4278)
                     /locus_tag="DHV81_04930"
     CDS             complement(3769..4278)
                     /locus_tag="DHV81_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCI93831.1"
                     /translation="MSVTEKKNNGGFSLVELIVVIAISVVLIGAATISIRSVMGVEVK
                     QCARNIESIINKTRVTTMGKDSAVLEIYKDASDGAYYYKTTINGTVSAPSKIGKSRID
                     VYYSMKDDYSDKTQLNGSDSIVLQFDRSSGAQKPDANNKYCSRIWIQKNSTEKVITIY
                     KETGKVVCE"
     gene            complement(4366..6687)
                     /locus_tag="DHV81_04935"
     CDS             complement(4366..6687)
                     /locus_tag="DHV81_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93832.1"
                     /translation="MKRNDNRGASFVMVVVAMAIVAVLAVTVLWIALMNLQMKVTDEK
                     NTDNFYSAEGVLDQICTGLQGDISKAYSAGYTKVMENYSDSSINEAGRQSIFAQEYLK
                     SLKGSLESDNTGMHYKTEKLKDYVDSKLTDENSKPHAVVKAVNADENGNGLLKVYNSR
                     AVINGIRVEYTDEKGFKSIIETDISLGVPSMSFTASGGVPELFTYSLVGNEGLEITSG
                     LNKVNLSGNLYAGCKNAADTGTSTTSVIIPNNATLDVKDTSYMIAEGDIEVGDFAGKD
                     TGTIKKNMAGVHNNSTLSVDSQCQLWTSNINVNGATVNLDGMTYVADDMTLKGTGSNV
                     TLGKNNKNNNAKYVGFGNGGANKENPVAGDSSAIIINGRKASLDMSNIRELMLAGTAY
                     INTQAIVNSSSSVGTQNSNVAMGESISVKGDQIAYLVPPECIGTSGDGADAKSLYNKN
                     PLSFKEYKDITATGQDQTKYTEINANVVSTKTGKALSAYLPEKKTIDNCIKKVFVPST
                     DNSDDGLVYYYVNLPTNKAAEYYSDYFGADSDKLNRYTKFYTDSIQVNEAASIYTAGN
                     YSIYDGSNLRLLKGIADGVDMDAQSDALQRTYTALNSKLLTDYADLPTNAASVSLFTN
                     IVNNTNLLKIVPNSGNKHTFETEYQGKKYQAIVINGNYTYDANDKSHNVDIIVATGNV
                     TLEADFTGTIVAKGRIIVKKDQCEIDNGTQEVFKALLLEKVSDADDALHLYDVFMDGA
                     NYLGNVSSFSNKKEADTKIDYATLITYQNWTKE"
     gene            complement(6690..>7064)
                     /locus_tag="DHV81_04940"
     CDS             complement(6690..>7064)
                     /locus_tag="DHV81_04940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93833.1"
                     /translation="IVKQKTISDQSELSTKESTYKAYVKVSEPGNRTGHAYTHIATNL
                     NVNMGAPDNPLQPDQAIYGFNNNVIQNDVKAIVDIKNLTKSNASERLYDVKVAVYESK
                     ASLDDIFKDKDPVVTMTGSMGY"
BASE COUNT         1778 a         1695 c         1189 g         2402 t
ORIGIN      
        1 catactcgtc ctttccaaga gtatcatttt cttttttcct gcttgtattc tctgagttga
       61 tatactctgc tatctcgtca aaattttcat ttttagctgc ttcgacaaat tgagtttcat
      121 cttcatttgc aataaatgca gaagcaaaat catctcttaa aacaaatggc acatccgtac
      181 taggattata gtttcgtgga atgacatatt cgttgccaat tacaaaacct tttgtatggg
      241 ttttactgtc aactttataa tactttatgt cattattcca attaataaaa ttatcagctg
      301 agtccttagt aaaattcagc gagctgaaat tgttacagct caaaagacct gcaagctttt
      361 taatattact tgagtagtct gcacttgaag cagtccacac aataccatta tccactataa
      421 caggcatcat tataccactc tggtgcacac gctggactat ctgctttacc ttgtcccatg
      481 aaatgtcatt atcagtttta tatgttgcag catcgtttac taaatcattt gaaaggacgg
      541 actttgagcc ggaaagatag agtaaatcaa ttgttgacac atctatctgc tcaagctcct
      601 taggtgtgac agtttttaca ttgaaggtca tattggcagt cttatcatca tcgcctttct
      661 tgccctcctg gttaaataca ccgtttctga accagttatt gttcttgaaa ccacccttat
      721 aatatatgtg tgtggtattt acaggataat caagcgagcc gtttttatca gccaccatta
      781 tatagtcacc ttttccctta cccacatatg tatatgtctg gctgtcctta actatatcaa
      841 aatatccgtc tgtatcggtg aggattaccc tgtctgtgtt accatcatta ttctctgtag
      901 cagaatcagc tattgaagca tcatctgaaa tatcatctgc gccggtctta cctgccggct
      961 gattgtccgg tgtgccatca tctatcttct cgtatggatt ctgtgaatca agcactgctc
     1021 catactcgcc ggtctcatcg taattaaact caataggtac ctcagtgtcc acctcgtagt
     1081 aggtctcgtc atcagtgatt ttatctgcag gcacatactc catctcaacc gtaaagtatg
     1141 tatacttctc ataatccggc ttactgccat caggcagact tgccatccgg tcagctcttg
     1201 caacaaattc atatggggat gtagtatccg atttgtcagt cctgtaaata tatgcctttg
     1261 ggtcatattt tgctatgttt tgcatgccct cagcagaaac aatggcatat gcgctcttta
     1321 tagaataaac cacatgcttc tcaacggtat cgtcacctgt gcctgtatct gtcgtattat
     1381 ctgtgctgct atcgatgtta ccatctgtac cgctgtctgt atttccatca gctttctgtg
     1441 aattatcagt ctctaccaga tactgggtat ttgtcatgcc atccggcaat ttgactgcag
     1501 taaacgcaac actgtagtaa cctctttttc ctgtatccac agattcagag ccatcttctg
     1561 tctcatcctc gccctgcatg aaaacataat ggtcaacatt ctgtctgtaa tgacctgtag
     1621 gcagtccatc ttcatttaca gcaagctggt aatcggtatt gaacttataa tctccctccg
     1681 cctgctctgt catcttataa ccggtggtac ccttgtttat tctgtccata tctacaaggg
     1741 caagctcact ccagtcatcg tctaaactga catagctctc ctcatacggc tcatagtaaa
     1801 gaggctttgt gttatccttt ttctcggtag taataggtgc aagctttgtt ttcagacctg
     1861 acatatagtc agcccttttt tgtacaccgt caccctcttt gtcagacatc tttgacagtg
     1921 ttccaaacca atcgtcaggc tcctgaccgt caatcagata gccaatggat gccattttct
     1981 tgtcaggcac cacctccact atattgaagg gcttgtcctt gccatgtgag ctgactatat
     2041 cagatattcc attgaatgct acatttgcat cgacctttgt taggctgtca ttcagcacaa
     2101 aagctgccac aacgagacat cctgtaagtg tgagtgatat tggaaccact attttttttc
     2161 ttaatttgct cattgcgatt gctcctttgt aaaaaactac tctgtttctt aatatgtata
     2221 aaacgtaatt gtatttgact taaactccgg catatcctca agtccaagcc agacgctgat
     2281 atttaatttc tctccggtaa gaaaatcatt tccgagagtg atatgcattt ctttattata
     2341 acctaaaata taataattaa gatagttctt atatttagga tcatctggct taccttgtgc
     2401 tcctgtttct ccggcaatct catttgaact aaacttaaat attccatcag gctcaccatt
     2461 gccctccggt ccataaatca gcttgccatc atcgcccatt ctaaaggata tatctccctt
     2521 gtgctcggtt gcacctgttg agctgacact ttctacctcc atatgccata caaaatcctt
     2581 aacctgatat ttaccattta atgaagaatt ttttaactcc cattcatcaa taaacaattg
     2641 atgcgtctgt cctaaattga aatgcttatc tgcatcacaa tgcagaatat tgattggatg
     2701 tgctacgttt acagttacct ttgccgtcaa tgacgtatcg tctttggatg ttactgtgat
     2761 aacaaattga tctgtagttt cattatccat aagttctaca ttatatacat caccattagc
     2821 attaaagttt ccaccatttt ttgttccgga atgatcccac gttacatttt ttgaatcata
     2881 ctttccaccc agaatcttat cagtaaactc taatttattc tgatttttct tcatttcata
     2941 gtataccgtt ccgttggtta catccttgcc tttataatat atccttattc ctttaggctc
     3001 tgccggctcc acatactcca catctgctcc tgtcggtacc ttatttcgca tagtgaagtt
     3061 ctgctttgat gtatacttct tgtcccctgc tttaaagtcc acgacaagtc cgacagagtt
     3121 cttgctttct gcctcgctta aatcaggtgt aaaacctgtg acattttctg ccataagcga
     3181 ttccttgccc tgaccatctg ccttcaccgc acctgttgca ggatccagtg tatcctttcg
     3241 cagatagatt ttgtgctctg atggtctgta tatgatattg tatgtacaat ccttgttgta
     3301 gataatcagc ttgctgtcgg atgatgtttc ctcagatgat gcttcttcgg atgacgcttc
     3361 ttccgctgat gcagttgcaa cataaccggc ctcaggggct gctgatgcca cctccaccgg
     3421 cgctgccact gtagcaggct catacttgac acccttttgc gcatcaatta tcaggtctcc
     3481 tatctgattc atcgtaagct gtgcctcata ctgtaaatct acatcctttg aagcgtactt
     3541 atactgcttt gtgctcgtac cgataaagcc tgcaacagcc agccccagta ttcccattat
     3601 cgccatgaca atgacaagct caacaagtgt aaagccactg tttttcctat tatttgattt
     3661 gttcatgcgc cttaaaagtt tcatggttca tcttcctctc cgatttcatc atacaacctt
     3721 ataacagcct tgcgtcaaaa taaaatattc atcattaaac tatgtaaatt actcacatac
     3781 caccttgccg gtctccttgt atatcgtgat aaccttctcg gtgctgtttt tttgaatcca
     3841 gatacggctg cagtacttgt tattggcatc aggcttttgt gcacctgagc ttctgtcaaa
     3901 ctgaagcact atactgtctg agccgttcaa ttgtgtctta tcactataat catccttcat
     3961 ggaataatat acatcaatcc tgctctttcc gattttgctt ggagcagata ctgtaccatt
     4021 tatggtcgtc ttataatagt aggcaccatc tgatgcatcc ttgtatatct caagaactgc
     4081 gctgtccttt cccatagttg tgacccttgt tttgttaatt atcgattcaa tatttctggc
     4141 acactgcttc acctcaacac ccatgaccga gcgaatggat atggttgccg cacctatgag
     4201 tacgacagat atcgctatca ctactataag ctctaccaga gagaaaccgc cattattctt
     4261 tttttctgtt acagacatat tggattcctc ccttatatat atcgtcaaaa tttgtcaaat
     4321 ataaatattt cgcagctgtt ttgcgcctta aaagccgctt gttttctact ccttggtcca
     4381 gttctggtat gtaatgagcg tagcataatc tatttttgtg tccgcctctt tcttatttga
     4441 aaaagaagaa acattaccca gatagttggc accgtccatg aacacatcat acaggtgcag
     4501 tgcatcgtct gcatcactta ccttttcaag aagcaatgcc ttaaatacct cctgagtacc
     4561 attatcaatc tcacactggt ctttttttac aattatcctg ccctttgcaa ctatggtacc
     4621 tgtaaaatca gcctccagtg tcacatttcc tgtagcaaca attatgtcca cattgtgtga
     4681 tttgtcattt gcatcatatg tgtagttacc gtttatgaca attgcctggt atttttttcc
     4741 ctgatactca gtctcaaatg tatgtttatt accggaattt ggcactattt tcagcaaatt
     4801 ggtattgtta actatatttg taaaaagcga aacacttgct gcatttgtcg gtaaatccgc
     4861 ataatccgtc aaaagctttg agtttagtgc ggtatacgta cgctgtaagg catctgactg
     4921 tgcatccatg tccacaccat cagctatgcc cttcaaaagt cttaaattgc tgccatcgta
     4981 gatgctgtaa ttgccggcag tatagatgct tgcagcctca tttacctgta tgctgtcagt
     5041 atagaactta gtgtatctat tgagcttgtc actgtctgca ccaaaatagt cactgtaata
     5101 ctcagcagcc ttgttcgttg gcagattgac ataatagtat acaagtccat catcactatt
     5161 atcagtagaa ggaacaaaga ccttctttat acagttgtcg attgtctttt tctctggcag
     5221 atatgccgag agagccttgc ctgtctttgt gctgaccaca ttggcattta tttcagtata
     5281 ctttgtctga tcctgtcctg ttgccgtaat atccttgtac tccttaaagg agagtggatt
     5341 cttgttgtaa agcgatttgg catccgcacc atctccactt gtgcctatgc actcaggcgg
     5401 cacaaggtat gctatctggt caccctttac agagattgac tcacccatgg ccacattgct
     5461 gttttgtgta cccacactac tacttgagtt tacaatagcc tgggtattga tatatgcggt
     5521 tccagccaac ataagctccc tgatatttga catatcaagc gatgccttcc tgccgtttat
     5581 gataatagca gagctatcgc ctgcgacagg attttccttg ttagcgcctc cattgccaaa
     5641 gccgacgtac ttcgcattgt tattcttatt attctttcca agtgtgacat ttgagcctgt
     5701 accctttaag gtcatgtcat ctgccacata tgtcatgcca tctaaattga ctgttgcacc
     5761 atttacattt atattgcttg tccacagctg acactgtgaa tcaacagaaa gtgtactgtt
     5821 attgtgtaca cccgccatgt tcttttttat tgtaccagta tcttttccgg caaagtctcc
     5881 cacctctata tcgccctctg caatcatata gctggtatcc tttacatcaa gtgtagcatt
     5941 gtttggaata ataacactcg tagtggaagt tcctgtatct gccgcatttt tacagccggc
     6001 atagagatta ccggataaat tcactttatt aagtcctgat gtaatttcca atccttcatt
     6061 tcctacaaga gaatatgtaa aaagctccgg aacacctccc gatgctgtaa agctcatgga
     6121 tggaactccc agagaaatat ccgtctctat tattgattta aatccctttt catcggtata
     6181 ctctaccctg atgccattaa tgactgcgcg gctgttgtat accttgagca gtccatttcc
     6241 attttcatcg gcatttaccg ccttgaccac agcgtgtggc tttgagtttt cgtctgtgag
     6301 cttactatcc acatagtctt taagcttttc agttttatag tgcataccgg tattgtcaga
     6361 ctcaagactt ccctttaagc tctttaaata ctcctgcgca aatatactct gtctgccggc
     6421 ctcatttatg ctgctgtcag agtaattctc cataaccttt gtatatcctg ctgagtaagc
     6481 cttggaaata tctccctgca gaccggtaca tatctgatcc agcacaccct ctgccgagta
     6541 aaagttgtct gtattttttt cgtccgtcac cttcatctgg aggttcataa gcgctatcca
     6601 cagcacagtc acagctaaaa cagccactat agccattgca actacaacca tcacaaagga
     6661 agcaccacgg ttatcattac gcttcataat taatacccca tgcttccggt catcgtaaca
     6721 accgggtctt tatccttaaa tatatcatcc agtgaagcct tgctctcgta tacagccacc
     6781 ttcacatcat acagcctttc agatgcattg ctttttgtca gattctttat atcgacaatt
     6841 gcctttacat cattctgaat tacattgtta ttaaagccat atatagcttg atcgggctgt
     6901 agtggattat ccggtgctcc catatttaca ttcagattgg tggctatatg agtataagca
     6961 tgacctgtac gatttcccgg ctcacttacc ttcacatatg ccttatatgt actctccttc
     7021 gtgctcaact ctgactgatc cgatatagtc ttttgcttta caat
//
LOCUS       DPUF01000140            5214 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10177, whole
            genome shotgun sequence.
ACCESSION   DPUF01000140 DPUF01000000
VERSION     DPUF01000140.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10177"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="DHV81_04945"
     CDS             <1..1302
                     /locus_tag="DHV81_04945"
                     /inference="COORDINATES: protein motif:HMM:PF13533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93834.1"
                     /translation="QALVVGKDSIYKEGDTRYVYVKNDEGGREKRIITTGESDENYTQ
                     VTEGLKEGELVYYNSTDQVPTKYSKYTVTRSDFDIANYGVRYSRSDAGTITETCDYEG
                     QIVSVNVKQDDTVEKGMLLYVVDTGEGKAAITEAKNAIDSENESYAQKIADYDKQIKD
                     LSEGGSSAEENNRQDEKPDTAYDEQQLKLNLQLLNLQKKLACADHTYQLSQLQSAYDS
                     ISAGNDGKGNISVYAKNDGKVSKVSVKAGDNVEEGSDILQITGNSTDVLLVQVKDSNS
                     YKVYKDNIADVGERVSLELDGTTIYGSCIGFAGYGSVAQKGYINQDDKGVHITGNTDS
                     GYGNAAFYIKLDNGIPDDLTRASAVKFSYVSFKKVVVVPTSIIHKQVNPMDKDDISYY
                     VWKIDGDSVVKQPVILSDYTSGSNTLVLYGIDEGDEICLAS"
     gene            1287..4098
                     /locus_tag="DHV81_04950"
                     /pseudo
     CDS             1287..4098
                     /locus_tag="DHV81_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516832.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     assembly_gap    3566..3616
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4101..4158
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4176..4916
                     /locus_tag="DHV81_04955"
     CDS             4176..4916
                     /locus_tag="DHV81_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HCI93835.1"
                     /translation="MENAIIAQNIKRYFKAGDGTTVHALDGVSMEIKKGTLAILKGRS
                     GSGKTTLLNILSALDMPTEGSVQFLDKNLEQMTEKDREMLRRYEMGFVFQSIALIPTM
                     TAYENVEFALRLAKNKRQIQKNLTDMDNDIGDAKNDVINENTHDRVSELLERVGLSKR
                     MDHMPDQLSGGEQQRVAIARAVVHRPKVVFADEPTGALDTASGFEVMRLFRELIDDED
                     ITIVMTTHDPNLMELGDEVFELSDGIVI"
     gene            4937..>5214
                     /locus_tag="DHV81_04960"
     CDS             4937..>5214
                     /locus_tag="DHV81_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93836.1"
                     /translation="MINCDGLVKIYETDDKKVMALEGLDLTVETGEMLAVIGKSGSGK
                     STLLNMIGGLETPTAGVLTIDGKDISTYSEAEMVRYRRDKVGFVWQKSA"
BASE COUNT         1597 a          892 c         1326 g         1290 t
ORIGIN      
        1 caggcacttg tcgtgggaaa agattccatc tataaggaag gcgataccag atatgtctat
       61 gtgaaaaatg atgagggcgg ccgcgagaaa cgcatcataa caacaggtga gtctgatgaa
      121 aactatacac aggtcacaga gggcttaaaa gagggggagc tggtctacta taattctaca
      181 gatcaggttc cgacaaagta tagcaaatat acggtaacaa ggtctgattt tgatatagca
      241 aactacggtg tgcgctacag cagatctgac gcagggacga taaccgaaac ctgtgattat
      301 gaagggcaga tcgtatcggt caatgtgaag caggacgata cggtagaaaa ggggatgctg
      361 ctttacgtgg tggataccgg agagggcaaa gctgccataa cagaggcgaa aaatgcgatc
      421 gactcggaga atgaatccta tgcgcagaag attgctgatt acgataaaca gataaaagat
      481 ttatctgaag gtggtagtag cgcagaagaa aataaccggc aggatgaaaa gccggataca
      541 gcatatgatg agcagcagtt gaaactgaat ttacaattat tgaatctgca gaaaaaactt
      601 gcctgtgcag accacaccta tcagctttcc cagctacagt cagcatatga cagcataagc
      661 gcaggaaatg acggcaaagg aaatatttca gtatatgcca aaaatgatgg aaaagtttca
      721 aaggtgagtg tcaaggcagg agataatgtg gaggaaggct cagatatcct gcagataacc
      781 ggaaattcca cggacgtact gctggttcag gtaaaggata gcaatagcta taaagtatat
      841 aaggacaata tagcagatgt gggagagcgc gtatcacttg agctggacgg tacaacgatc
      901 tacggaagct gtatcggttt tgccggttac ggtagtgttg ctcaaaaggg atatataaat
      961 caggatgata aaggtgtgca tattaccgga aacacagatt cgggatatgg aaatgcagca
     1021 ttttatataa aacttgacaa tggaataccg gatgatctga ccagggcatc tgctgtaaag
     1081 ttttcttatg tttcttttaa aaaggtggta gtggtgccga cttcgatcat acacaagcag
     1141 gtcaatccga tggacaaaga tgatatatcc tactatgtgt ggaaaattga tggggacagc
     1201 gtggtaaagc agccggtaat attgagcgat tatacatcag gcagcaatac acttgtgctt
     1261 tacggtattg atgagggcga tgaaatatgt ttggcttcgt aattaataaa ttaaaaaaca
     1321 gaaaatggtt aaatttgtgt ctgcttatgg gagtggcttt gtttatagca ctttttgtgt
     1381 gcaaccctat gtttgaaaag ggcgcgggca atcagatact cgacagactt tttacggatc
     1441 atgccacaca gcagaatgag tttcctgcac agatgagccg cgagggaaca tatgcggtgg
     1501 cagattatcc tgattcgcag tcagtgcttg ataagctgtc aggatacgaa aagaagtgga
     1561 ctgaatatgt ggatataaac aaggtttcct cacagcagct tatcaaactc tcaggaggac
     1621 gcgcagatac agagtttgga ggacttaacc attattttac aataggatat ctgccggggc
     1681 tttcggagta cgcggatgtt gtaaaatctc agactgacac agccacattc gaatgcagta
     1741 taagcgaaaa gacaatggat cactacggac ttgcagcagg tgaaaaagtc tatttacatg
     1801 tacctgatgg cagtgggaaa aaggaaattt ctttcgtcat atcacagatc tgccgtgaaa
     1861 aagatataaa cgatccatat tgggccaaga cgctttcgga tatgggcgat gtgatatttg
     1921 tttcgcagga tgtttttgac gaaatgatgc agtattacag cgaggacaat atcagctaca
     1981 gtgattttct catgctggat tacaggcaga tcaacacgga aaatgcctca ctttatgacg
     2041 gttatatgga gcagtttaag gatgctgaca agctctacaa tgacaacatc cgtgatacgc
     2101 tggagcgctt ttttacacag cagaagacgg taaagctgat gctttgggcg ttggagctgc
     2161 cgtgtcttgt gcttttgatt ttatttataa gaatgatatc tgcacagata ctttcgctcg
     2221 aggaaaatga catacttgtt ttaagaagcc gcggtgcgac aaaattacag gtatttattt
     2281 tgtacttaca gcagtcggga ataatagcga tcgcgggctg tgtactcgga atagtactcg
     2341 gatatataat gtgccgggct gccgcatcta cagacggatt tttaagattt acggcaaagg
     2401 atataagcac ttacaaattt gtatggcaga tgcttgtcta tgcggtggca gcggcagtta
     2461 tcatggtgct gtttatcaca gttccggtgt ggaaaaaatc aaaggatgcc atatcagagc
     2521 gcagcagtag gggacgaata tcaaagaaaa agcctctttg ggagaaatgc tttatagatg
     2581 ttatactttt ggctctgtca gcatatctgc tttacaatta caacaagcag aagagtacgc
     2641 ttgcgatatc tgttcttgag aacaggagca tagatcctgt catgatactg gacaattcgc
     2701 tttttatatt tgcagcggca cttttgtgcg taagacttac cggacttatt attctgcttg
     2761 tggacaggct ctttaaaaag catttttctc cggcaatgta tgcatcattt ttacagctaa
     2821 aacgaaccag atacaatcag ggatttctgg cagtatttct tatcatgaca gtggctggcg
     2881 gtatttttga cgcgaatatg gcgcgcacca tgaacagcaa tatggaaaaa cggatcgtct
     2941 acaatgtggg ttgtgatgcg atatacaacg aagatttcag actcaggata cagaagaata
     3001 aagatggctc tgtaagctgg atgtatgacg agcctgattt cggtaaatac gagtcgctca
     3061 aaaatgaggg catctgtgac ggggtcacgc gtgtgcttga ggacgagcgg gtagacattt
     3121 ccgcaggcag tggttcggtt tcggatgcat atcttatggc gataaacaca aaagaattcg
     3181 gtgagactgc gaaattgcag agtgaccaaa atgatgatat taatgactct cactggttca
     3241 attacctgaa tgagctggca aaagagccgg acggagtcat catttcgtca aatctggcaa
     3301 aagatctggg acttaaagtt ggtgacagct taaattactc aaggtatgta ccggaggctg
     3361 tcgatgaaga tcagatctac gcatccgaga atgtaaaggt atgtgccata gtaaaaggtt
     3421 ttccgggcta cgagcgttac acctatgaga cagatgcaga caaaaatgtg accgagcagg
     3481 agaaatacct gatcgttgca aattatgcta cggttgtgga aaagttcggc atgacaccgt
     3541 acagtgtgtg gatgaacctg tcaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnntaac atccgcgcag cagcttatag acgagaataa aaactcagcc
     3661 acagtgcaga tcacaaacgg tatgtttaca ttaagtttta tactttcact gatagtgtgc
     3721 tcagtaggat ttttgctgta ttgggttatg acactaaaac agagggagct gcagtttggt
     3781 atataccgcg caatgggaat gagaatgaga gaggtaaaag caatgctttt aaacgagcag
     3841 atatttttat catttctgcc gcttcttgcg ggagcaggca taggtataac tgcaacagcg
     3901 atgtttgtaa ggctcatatc gatcatatat ctgccgcaga aacacaatat cggagtaaat
     3961 gtatatattt atccatctga tatgctggag cttacaggag tacttttcgt ggctgtagcc
     4021 gtatgctatg tggttatcag cagactgctt aagagtatga agatagcaca ggcactaagg
     4081 cttggagaag atagttagca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnaa atgaggtcgt gacatatgga aaatgcgata attgctcaaa
     4201 atataaaaag atatttcaag gcgggagatg gtacaacagt ccacgcactt gacggagtat
     4261 ccatggagat aaaaaaagga acacttgcca tcttaaaagg gcgttccggc tccggaaaga
     4321 ccacacttct taatatcctt agtgcgcttg atatgccgac agagggaagt gtgcagtttc
     4381 tggataaaaa tctcgagcag atgaccgaga aagaccggga aatgctaagg cggtatgaga
     4441 tgggatttgt attccagtct atagcactaa ttcccacgat gacggcatat gagaatgtgg
     4501 aatttgccct tagacttgca aaaaataaac gtcagataca aaagaatttg actgatatgg
     4561 ataatgacat tggagatgca aaaaacgatg tgataaatga gaatacgcat gacagagtat
     4621 cagagctgct ggagcgtgtc ggactctcaa agcgcatgga tcatatgccg gatcagttat
     4681 cgggaggtga gcagcagaga gttgcgatag cgcgcgctgt ggttcatcgc ccgaaggtgg
     4741 tctttgcgga tgagccgaca ggagcactcg ataccgcttc tggatttgaa gtcatgcgat
     4801 tgttccgtga gttgatagac gatgaggaca tcacaatagt tatgaccacg catgatccga
     4861 acctgatgga gctgggggat gaagtgtttg agcttagtga cggcatagta atttaaatta
     4921 aaagggggaa aagtagatga taaactgcga tggactggta aaaatatatg agacagacga
     4981 taaaaaggtg atggcactgg aggggcttga cctgacggtg gaaacagggg agatgcttgc
     5041 ggtaatagga aaatccggca gcggaaaatc caccctttta aatatgatag gaggactgga
     5101 aacaccaacg gctggagtgc tcactattga cggaaaagat atttccacat attcagaagc
     5161 tgaaatggtg agataccgca gggacaaggt gggctttgtg tggcagaaaa gtgc
//
LOCUS       DPUF01000141            4405 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_33923, whole
            genome shotgun sequence.
ACCESSION   DPUF01000141 DPUF01000000
VERSION     DPUF01000141.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_33923"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="DHV81_04965"
     CDS             complement(<1..1916)
                     /locus_tag="DHV81_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCI93837.1"
                     /translation="MDVKWTSEQKKVIDLRDRDILVSAAAGSGKTAVLVERIVNRICV
                     DNPPVDIDRMLVVTFTKAAAAEMRERVSRAIDSLKEQKPDDENLQRQSTLVHNALITT
                     IDSFCLFVVQNNFAQLNLDPDFRIGDQAELKLMLKDALAQVFEDNYAREDNEAFINLI
                     DTYSKGRNDSAVRQMVEDIYYKAGSSSWPRKWMNSLLRLYDIKSAKQLEDSEIIKEIV
                     DYSRVLLEEAVQELTMAKDLASATPGLEKYALTLSEDIALFDGMADVTGYVGMQEFLN
                     KISFGRIAVIRKFDGDEKKKERVKSMRDAAKKKIDGIKQKYFGMSIELMYEQLERQRP
                     FVKELIRLSLEFYDAMEAVKTRKRVFDFSDIEHFALRILVDEQTLEPTETAREFSKHF
                     EEIMIDEYQDSNQVQEDILTAISREHQGVGNMFMVGDVKQSIYRFRMARPELFMEKYN
                     TYTSDDSAHQRIDLHKNFRSRNEVLDFTNDIFYKIMAADLGNVQYDDDAALYPGASYP
                     EETMRPELLLVDYKDEDLSEIIEDEDGDKVQIEALLIANRIRSLMENGMVTDKKTGQL
                     RAVQYRDIVILSRSVATWGNTVAAVLKDCDIPAHVESNTGYFSSYEIQVILSMLRILD
                     NPLQDIPMAAVLASP"
     gene            complement(1931..>4405)
                     /locus_tag="DHV81_04970"
     CDS             complement(1931..>4405)
                     /locus_tag="DHV81_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCI93838.1"
                     /translation="NENLRYRDIAIVTGDVNIYAGYVREIFDKYNIPYFIDATQEILF
                     HPFIEFIRSIPDIAAQNFSADAVFRFLRCGFSELLDSEIDVLENYVLACGVRGKSAWD
                     KKWVRLPKHKAGYDLELLNQSREYIMELLKPVYQVFSDKKSTVKDYVLAIYKLIVLLD
                     IPDKLAKKEQALLDAGDQTASKEYGQIYKIVMQLFEKYVAVLGDECMDAEEFTQILDA
                     GLDAADVAVIPPGYDTVTIGDIERTRLTNIKVMFFAGVNDGIVPKAAGRGGIISQYER
                     EALKELDIELAPGAREQAFIQRFYLYLNMTKPSRELYISYTRLDSEKKAAQPSYLIGI
                     LKGMFPELVVTETEDVEQLLDISSRQSALDYLLSNEVDDRWCEIAAAVMLYDASVSDA
                     GECNEVDDKEFTQKNSVERLINARFEHYSKDPISRNVARSIYGRHMEGSITRFEQFAR
                     CAYAHFLNYGLRLTEREESGFTSLDMGNIYHEALERYSKKLDRGSTDWFSVTDAKRDE
                     LAMEAINEVIDEYAGFGIFDTAESQHSISHMKAVFKQTVWALTTQIRRGSFVPERFEF
                     SFYESLDMANDVTVDIKGRVDRTDTYTDEGRLFVKVLDYKSGNTVFDLVKLYYGTQLQ
                     LAVYMDAVMEQKKEALKQVSVEPGGMLYYHIDDPVIDLKDVTPGTELSEEEINQMILK
                     NLKPDGLINSDEKAYRGMDRDFEKSSDVIPVTLKKDQTPAAGSKVANAEEFDVITRFA
                     REKLREIGREIYDGNVAVNPIKNKKIDSCAYCAFQAICRYDSRIPGFSERSFNGDNKE
                     DILDKMRTQIAAAEHKACYGDNNIKP"
BASE COUNT         1070 a         1210 c          764 g         1361 t
ORIGIN      
        1 ggcgaagcaa gcactgctgc catcggaata tcctgcagcg gattatcgag tatgcgaagc
       61 atggacagaa tcacctgtat ctcatacgat gaaaagtagc ctgtattcga ctctacatgc
      121 gccggaatat cacagtcctt taatactgct gccactgtat tgccccatgt tgcaacactt
      181 ctcgaaagta tcactatatc cctgtactgc acggctctta gctgccctgt ctttttatca
      241 gtcaccatgc cgttttccat cagactcctg atacggtttg caatcaaaag cgcctctatc
      301 tgcaccttat ctccatcctc atcctctata atttcagaca gatcctcatc cttgtagtca
      361 acaagcaaaa gctcaggacg catcgtctcc tccggatagc ttgcaccggg atacagtgcc
      421 gcatcgtcat catactgcac attgcccaaa tctgctgcca tgattttgta gaatatgtcg
      481 ttagtaaagt ctaacacctc attcctgctt ctgaaatttt tatgcaggtc tattctctgg
      541 tgtgcactgt catctgatgt atatgtgttg tatttttcca taaacagctc aggacgcgcc
      601 attcgaaacc tatatatact ctgctttaca tcgcccacca taaacatgtt gccgactccc
      661 tggtgctctc ttgaaatagc ggtaagtata tcctcctgca cctggtttga gtcctgatac
      721 tcgtcaatca tgatttcctc aaaatgtttt gaaaactcac gcgctgtctc tgtgggctcc
      781 agtgtctgtt catccaccag tatgcgaagc gcaaagtgct ctatatcaga aaaatcaaat
      841 actctttttc ttgtttttac agcctccatc gcatcataaa actcgagtga gagccttatc
      901 aactccttaa caaatggtct ttgacgctcc agctgctcat acataagctc aattgacata
      961 ccaaagtatt tctgctttat tccgtctatc ttcttcttgg cagcatcgcg catgcttttc
     1021 acgcgctcct ttttcttctc gtctccatca aatttcctga tgacagcaat tctgccaaag
     1081 gatattttgt ttaagaactc ctgcatgccg acatagcctg tcacatcagc cattccatca
     1141 aacaatgcta tatcctctga aagtgtaagc gcatactttt caagccctgg tgtagctgat
     1201 gccaggtctt tcgccattgt aagctcctga acagcctcct ccaaaagcac cctgctgtaa
     1261 tccactatct ccttaatgat ttctgagtcc tcaagctgtt tagctgactt tatatcatag
     1321 agtcttaaga ggctgttcat ccactttctc ggccatgatg agctgcccgc cttatagtag
     1381 atatcctcca ccatctgcct gacggcagag tcatttctcc ccttgctgta ggtatctata
     1441 aggttgataa atgcctcgtt gtcttctctt gcataattgt cctcaaatac ctgtgccaaa
     1501 gcatccttta gcataagctt aagctctgcc tggtcaccga ttctgaaatc aggatccaga
     1561 ttaagctgtg cgaaattatt ctgcaccaca aacagacaaa acgaatcgat tgtagtgata
     1621 agcgcattgt gaaccagtgt cgactgcctc tgcaggttct catcgtccgg cttttgctcc
     1681 ttcaagctgt ctatcgcacg gctgacacgc tccctcatct cagcagctgc agccttggta
     1741 aacgtcacca caagcatcct gtcgatatca accggcggat tatccacaca tattctgttt
     1801 actatacgct caacgagtac agctgtcttt cctgagcccg ctgccgccga tacaagtata
     1861 tccctgtctc ttaaatctat aacctttttc tgttcactgg tccatttaac atccatatta
     1921 tgtctcctga ttatggcttt atattattat ctccatagca agctttatgc tctgccgctg
     1981 caatctgtgt acgcatttta tcaagtatat cttccttatt gtcaccgtta aagcttcttt
     2041 cgctaaagcc cggtatcctt gaatcatacc tgcatatggc ctgaaaagcg caatatgcgc
     2101 agctgtctat tttcttgttc ttgataggat tgacggcaac atttccatcg tatatttctc
     2161 ttcctatctc gcgaagcttc tcccgcgcaa accttgttat cacatcaaac tcttcggcgt
     2221 ttgccacctt tgagcctgca gccggtgtct gatccttttt taaggtgaca gggattacgt
     2281 cagagctttt ctcaaagtcc ctgtccatgc ctctgtatgc cttttcgtca gagttgataa
     2341 gcccgtcagg cttgagattc tttaatatca tctgattgat ttcttcctca gacagctcag
     2401 tgcccggtgt cacatccttt aaatcaatta caggatcgtc gatgtgatag tacagcatac
     2461 cgcccggctc cacacttacc tgctttagtg cctctttctt ttgttccatg acagcgtcca
     2521 tgtacactgc aagctgaagc tgcgtgccat agtacagctt caccaggtca aaaactgtat
     2581 ttcccgactt gtaatccagt accttcacaa aaagcctgcc ctcatccgta tatgtgtcag
     2641 tcctgtccac gcgtcccttt atatcaaccg tcacatcatt tgccatatca aggctctcgt
     2701 aaaaggaaaa ctcaaacctc tcaggcacaa agctgcctct tcgtatctgg gttgtgagtg
     2761 cccatactgt ctgcttaaag accgccttca tatgtgatat cgaatgttgg ctttccgccg
     2821 tatcaaatat gccaaagcct gcatactcat caatcacctc atttattgct tccatcgcaa
     2881 gctcatccct ttttgcatct gtcacgctaa accagtcagt actcccccta tcaagcttct
     2941 tactgtatct ttccagtgct tcatggtata tattacccat atcaagggat gtaaagccgc
     3001 tctcctcacg ctctgtaagt ctcaatccat aatttaaaaa atgcgcatat gcacacctgg
     3061 caaactgctc aaagcgcgtg atactgccct ccatgtgtct gccatatatg cttcttgcca
     3121 catttcggct aatcggatcc tttgaataat gctcaaacct tgcatttatg agcctctcca
     3181 cagaattttt ttgagtaaat tctttatcgt ctacttcatt acattcccct gcatcactga
     3241 ctgatgcgtc atagagcatc accgctgctg caatctcgca ccatctgtca tccacctcat
     3301 ttgacagcag ataatccaga gcactctgcc tgcttgatat atccaaaagc tgttctacat
     3361 cctctgtctc agtcacgaca agctccggaa acatcccctt taatatacct atgaggtacg
     3421 atggctgggc agcctttttt tcactgtcaa gcctcgtata gctgatataa agctccctgg
     3481 atggctttgt catattcaga tacagataaa acctctgtat aaatgcctgc tcccttgccc
     3541 cgggcgcaag ctctatatcc agctccttaa gtgcctctct ctcatactgg gatataatgc
     3601 ctcctcttcc tgccgccttt ggaacaatcc catcgtttac gcctgcaaaa aacatgacct
     3661 ttatgttggt cagacgcgtt ctctcaatat ctccgatggt cacagtgtcg tatcccggcg
     3721 gtatgactgc tacatctgcc gcatccagtc ctgcatccag tatctgtgta aattcctctg
     3781 cgtccatgca ttcatcaccc agcaccgcca catatttttc aaagagctgc atgactattt
     3841 tgtatatctg tccgtattcc tttgatgcag tctggtcccc tgcatcaagc agtgcctgct
     3901 ccttttttgc aagcttatca ggtatatcaa gcaaaactat cagcttatat atagcaagca
     3961 catagtcctt taccgttgac tttttatcgg aaaacacctg atataccggc ttgagcaact
     4021 ccattatata ctccctgctt tggtttaaaa gttcaaggtc atagcctgcc ttatgctttg
     4081 gcagcctgac ccactttttg tcccatgcgc ttttacctct tactccacag gcgagcacgt
     4141 agttttcaag tacatctatt tcactgtcca atagctcact aaagccgcac cgcaaaaatc
     4201 tgaacaccgc atctgccgag aaattttgtg ccgcaatatc gggtattgac ctgataaact
     4261 caataaacgg atggaaaagt atctcctgtg tggcatcgat aaagtaagga atattgtatt
     4321 tgtcaaatat ttcacgcaca taacctgcat atatattcac atctcctgtg actatagcaa
     4381 tatcgcggta acgcaggttt tcgtt
//
LOCUS       DPUF01000142            3122 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_27025, whole
            genome shotgun sequence.
ACCESSION   DPUF01000142 DPUF01000000
VERSION     DPUF01000142.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_27025"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="DHV81_04975"
     CDS             <1..696
                     /locus_tag="DHV81_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCI93839.1"
                     /translation="QAKGILSSANYFGTMLVKMGIADALLGGATYSTADTVRPALQLI
                     KTKPGNTIVSSCFILVRPSATGENEVLAMSDCAINIHPTEDELVEIAGESAECAKIFG
                     IDPKVAFLSYSTLGSGKGEDVDKMRNAAHKAKEKYPNLPIEGEIQFDAAVAPRVARTK
                     CPQSEVAGHANTFIFPDINAGNIGYKIAQRLGNFEAYGPILLGLNAPINDLSRGCNAG
                     EVYSMAIITAALA"
     gene            896..1429
                     /locus_tag="DHV81_04980"
     CDS             896..1429
                     /locus_tag="DHV81_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCI93840.1"
                     /translation="MRVGMGYDVHKLVEGRDLILGGVKVPHTLGLLGHSDADVLLHAI
                     MDALLGAAALGDIGKHFPDTDPAYEGISSLKLLEHVRDLIAKKGYVIENIDATVIAQK
                     PKLRPYIEQMEQNVADTLQIAKEQVNIKATTEEWLGFTGREEGIASQAICSLTGLYEA
                     SMQVGGGCSGCTGCQTD"
     gene            1500..2927
                     /locus_tag="DHV81_04985"
     CDS             1500..2927
                     /locus_tag="DHV81_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCI93841.1"
                     /translation="MENNFKFYNTLTRQIDTVVPRVDGKIGMYTCGPTVYHFAHIGNI
                     RSYIMEDVLEKALRYVGYDVKRVMNITDVGHLSSDGDTGEDKMLKGAKREHKSVMEVA
                     KEYTDAFFKDFTAVHNRMPDVVEPATNCIPEFIHMVEVLIEKGYAYFAGGNVYFDTSK
                     LDDYFVFSSAVEKEQLQVGVRDDVEEDVNKKNKNDFVLWFTQSKFEDQALKWDSPWGV
                     GYPGWHIECSCISMKHLGEYVDIHCGGVDNIFPHHTNEIAQSESYLGHDWCKYWFHVN
                     HLNDRAGKMSKSKGAILTVSVLQEKGYNPLAYRFFCLQSHYRKPLEFSFDALDNAVAA
                     YNKIAKRVAELKDEGDIDETAFSDFKKKFTDAVSNDLNTSMAITIVYDVLKADISDRT
                     KLALIDDFEQVLDLGLRESGKALLEASSSVDSELEAFINDKIAERAAAKKAKDFATAD
                     AIRDELLAKGVAIKDTREGVKWELV"
     gene            2929..>3122
                     /locus_tag="DHV81_04990"
     CDS             2929..>3122
                     /locus_tag="DHV81_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCI93842.1"
                     /translation="MKIDSYIKEQFGIKDVDIRTYSPLTLAYIGDGIFDIVIRSVVVG
                     KGNTKANLLHKHTSNIVKAHT"
BASE COUNT          956 a          593 c          789 g          784 t
ORIGIN      
        1 caggctaagg gaattctttc atcagctaac tactttggaa caatgcttgt aaagatgggt
       61 atcgcagacg cacttctcgg aggagctaca tactcaacag cagatacagt tcgtccggca
      121 ttacagctta tcaagacaaa gcctggcaac acaatcgtat cttcatgctt tatccttgtt
      181 cgtccatctg caacaggtga gaatgaggtg cttgcaatga gcgactgtgc tataaacatc
      241 catcctacag aggacgagct tgttgaaatc gcaggagaat cagcagagtg tgctaagatc
      301 ttcggtatcg atccaaaggt tgcattctta agctactcaa cacttggctc aggaaagggc
      361 gaggatgttg acaagatgag aaacgcagct cacaaggcaa aagagaaata tccaaatctt
      421 ccaatcgagg gagaaatcca gtttgacgca gcagttgcac cacgtgtagc ccgcacaaag
      481 tgcccacagt ctgaggttgc gggtcatgca aatacattta tcttccctga catcaacgca
      541 ggaaatatcg gatacaagat tgcacagaga cttggtaatt tcgaagcata cggcccaatc
      601 cttcttggac tcaatgctcc aatcaatgat ctttctcgtg gatgtaatgc aggagaggtt
      661 tactcaatgg ctatcatcac tgcagcactt gcataagaca tttaatggac tattccaatt
      721 ttaataaaga ccgctccggt ttgtccgggg cggtctttta tttgaaaata gcttaaaata
      781 atatcttggc gcaacaggag aaataaggta tactataatt aaaaaaccaa aatacagagc
      841 aatgacagtt ggcaataaca tgaggtgttg tatctgatag aaagagagac gatatatgag
      901 agtaggaatg ggctatgatg tgcacaagct tgttgaaggc agagatttga ttttaggagg
      961 agtgaaggtt ccgcatacac tcgggctttt aggccattcg gatgccgatg tactgttgca
     1021 tgcgataatg gatgcactct tgggtgcagc ggcacttggc gatatcggaa agcatttccc
     1081 ggatacagac ccggcatatg agggtatttc gagtcttaag cttttggagc atgtgagaga
     1141 cctcattgca aaaaaaggat atgtgataga aaacatagat gcgacagtaa ttgcacaaaa
     1201 gccgaagctt cgtccgtata ttgagcagat ggagcagaat gtggctgata cgctgcaaat
     1261 cgcaaaggaa caggtaaata taaaggcgac gaccgaggag tggcttggct ttacaggccg
     1321 tgaggaggga attgcatcgc aggcaatctg ttcacttaca ggtctctatg aggctagtat
     1381 gcaggtcgga ggcggatgca gcggctgcac cggctgtcag accgattgat ttgaatatat
     1441 atggaaaaca tgctaaaata aaaagatagt tataaatcat ccaaaaggag atataaataa
     1501 tggagaacaa tttcaagttt tacaatacac tcacaagaca gattgatact gttgttccgc
     1561 gtgttgacgg caaaatcggc atgtatacat gtggacctac agtttaccat tttgcacata
     1621 tcggaaatat ccgctcatac atcatggagg atgtgctcga gaaggcactt cgctatgtgg
     1681 gctatgatgt aaagcgtgtc atgaatatca ctgatgtggg acatctctca agcgacggag
     1741 ataccggaga ggacaagatg ctaaagggcg caaagcgtga acacaagagc gtcatggagg
     1801 tggcaaagga gtacacagat gcctttttca aggatttcac agccgtacac aacagaatgc
     1861 ctgatgtcgt agagcctgca accaactgta ttccggaatt catccatatg gtggaggtgc
     1921 ttatcgagaa gggctatgcc tattttgcag gaggcaatgt atatttcgat acatcaaagc
     1981 ttgatgatta ttttgtgttc tcctcagcag tagagaagga acagcttcag gttggagtgc
     2041 gagatgatgt cgaggaggat gtaaacaaga agaataaaaa tgatttcgta ctctggttta
     2101 cacagtctaa gtttgaggac caggctctta aatgggacag cccatggggc gtcggatatc
     2161 cgggctggca tatagagtgc tcatgcatta gcatgaagca tctcggagag tacgtggata
     2221 tccactgtgg cggtgtggac aatattttcc cacatcacac caatgagatt gcacagtcag
     2281 agagctatct gggacacgac tggtgcaagt actggttcca tgtcaatcac ttaaatgaca
     2341 gagccggcaa gatgagcaag tcgaagggtg caatcctcac agtttcggtg cttcaggaga
     2401 agggctacaa tccgcttgcc tacagattct tctgtttaca gtcacactac agaaagcctc
     2461 ttgagttttc gtttgatgcg ctcgacaatg cagttgcggc atacaataag attgcaaagc
     2521 gtgtggcaga gcttaaggat gagggtgata ttgatgagac agctttttct gactttaaga
     2581 agaaatttac tgatgcagtt tcaaatgatc tgaatacatc aatggctata accatagtgt
     2641 atgatgtgct taaggctgat atctcagaca ggacaaagct tgcacttatc gatgactttg
     2701 agcaggtact tgatctggga cttagggaat caggaaaggc cttacttgag gcatcgtcat
     2761 ctgtagacag tgagcttgag gcatttatca atgataaaat tgcagagcgt gcagccgcta
     2821 aaaaggcaaa ggattttgcg acagccgatg ccataagaga tgagcttctg gcaaaggggg
     2881 ttgccataaa ggataccaga gagggagtaa aatgggagct tgtatagtat gaagatagac
     2941 tcatatatta aggaacagtt tggaataaag gatgttgata taaggacata ctcgccgctt
     3001 acacttgcat atatcggtga tggcatattt gatattgtca tccgctcagt tgtggtggga
     3061 aagggcaata caaaggcaaa tctgcttcat aagcacacaa gtaatatagt aaaggcacat
     3121 ac
//
LOCUS       DPUF01000143            8563 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1888, whole
            genome shotgun sequence.
ACCESSION   DPUF01000143 DPUF01000000
VERSION     DPUF01000143.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1888"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="DHV81_04995"
     CDS             complement(<1..419)
                     /locus_tag="DHV81_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCI93843.1"
                     /translation="MSKIRTRFAPSPTGRMHVGNLRTALYAYLIAKHEGGDFILRIED
                     TDQVRQVEGAVDIINRTLEETGLVHDEGPDKDGGCGPYVQSERVAQGIYMKYAKELID
                     KGEAYYCFCDKERLDSLKTTVAGKEISIYDKHCLHLS"
     gene            complement(497..1645)
                     /locus_tag="DHV81_05000"
     CDS             complement(497..1645)
                     /locus_tag="DHV81_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93844.1"
                     /translation="MLFKRKKKTDDTQLHIESIPDESTKGFNVVEICEQMVDAARELE
                     DNRTEYALVTNYLTDIERMENMSETDKKTMNDAAANILTLEKTRSDFLKTKNRISDSQ
                     YAQMQQLEDEIPRAIKRLEKNESSLDVINKDLRYLEGEKVQFDIDGEYAKSRQRAFRV
                     YAVLLVVFFAVVVAVCTLMQIVYGADTTIFMLIGALLSTVAGSFVLLTYQSYSDEVKS
                     AAASKNKAVALENRVKIKYVSIKNAVDYTYEKYHVKNSKEFVYNYEQYLLAVKDKERF
                     RRTNEDLEYNSKKLVSVLSKNDFYDARVWLNYTNAIVDHKEMVEQKHELIVRRQKLRG
                     RMSCNVETITRLRREILSHRSELGDRIHEVNRILNKIAELDMEVQSED"
     gene            complement(1635..2414)
                     /locus_tag="DHV81_05005"
     CDS             complement(1635..2414)
                     /locus_tag="DHV81_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93845.1"
                     /translation="MGELLLCNEAIAAMPYYIEGVSINVYSIEELNYYIINNTYLLDD
                     DFMSLELCTWIENHAHLPKLAMHLRTIISTNGRLSAFVTELLEYSGYCTKEELKNTHD
                     ALKELEEKSEFECLKIRADRLMEKKKYTAAIFEYKKLLSCEDAKHTERELYGNIYHNL
                     ATAYVQLFLFDIAADLYLEAFRQNGDRKSLWSCLYAYKLDNNDIGYARIVREYSVTEQ
                     EINNLDSVLKGVYAEASDGCDEGRPKETILKYKEDYRAHVI"
     gene            complement(2423..3661)
                     /locus_tag="DHV81_05010"
     CDS             complement(2423..3661)
                     /locus_tag="DHV81_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93846.1"
                     /translation="MGHNGNSYYIGIDLNDSYAMVSFFQQNMKEPETVSTVAGSEEFQ
                     IPLVLARRKSIGKWYYGDEARRLSKSGEMVCIDQLLKRALNSEKIVIDDDSFMAEELL
                     ALFLKKVMELPSKLGNPSSFDRLVICVDRLTKENVSMFYGMAVRLGINSRQLTVIDRK
                     ESFYYFALNQDKSLWLHDVVMFMQEKESIFFYSLKRDLRTTPQVVSIDESISYTLDKN
                     DKDKSFLDIINESFKNQIISTVFLVGDTFAEGWMKQSVALLCKGRRVFMGNNLFTKGA
                     CYAAATRDREAEWPFVYMGENEMKFNLSLKVHDKGELSFYNLISAGSNWFESKGQCEV
                     IISGSYEVDFWKQLPKSREAKIETLRLTDMPPRPDRATRIRITATPVSDDRIDIEIKD
                     LGFGEIFMSSGKVWHYEMTM"
     gene            complement(3673..7218)
                     /locus_tag="DHV81_05015"
     CDS             complement(3673..7218)
                     /locus_tag="DHV81_05015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93847.1"
                     /translation="MRARIGQIAAGRFNGTKPILAFSEETIDLSVIEGRSEAGSFVIE
                     STNQIKICGIVYSTNPRMECLNPHFEGEKVRIRYQFNSKGLTEGDACEGKFVIVCNQI
                     EYSLSFCARITRLYAEASTGAVKSLDDFTRLAASNWDEAYHLFYNRNFLNTIPYDNVY
                     ERLTYEGFACARPSGQNMEEFLIGVNKKQPVSISVDKSEEIFMASKEPQSGCFTITKD
                     NWGYTEIRLRTDCEFIKLSKPVLTLDDFIGKTYLYEYIIDASAMHAGRNFGRIYIDGV
                     YQSFTIDITAGVRDDDGSISDIAVTKDIKECMVGIMELYTSFRLKRIVTGVWANETIS
                     ILNHLHALVPDEHMYELMKAQAFIINRQRQEAKWILDDFKHSNPDKKAPIWGYYLYLM
                     TLLEREPSYVDNMTHEVELIFYENPDSVLLFWVLLFLRDQYFDDSAGKLKDIKYWVLR
                     GCSSPYLYIEAYYLISQDPYLIKELSVFELRILSWAVKEKALTKELAGAIFEAVDLAG
                     GFDNRVYELLTAAYEICPEAEYVGIICSYLIKGHKNDTCFHKWFELGIENKLRLTGLY
                     ESYLLTMDDRQISPVPKIIQMYFSFDNKLPYRKLAVLYNNIIAAKETEPEVYHKYRKA
                     MGRFAMDQAQLRHIDDNLAVLYEDMLELGFINEELSAAFSDIIYTHKLIVFDKRIVRA
                     IIYQNEMKEPQIVPVTDQCAYFELFSNDYVILFEDSRGYRYVKSISYRLQRLMDAEKY
                     LDRCISLSPDRPQYIVSHFKHVRDYSDFTKDDLKLFKPVFYSESFSDSYKAVMGYRIL
                     KYCQLHDYEDYVRPFLQSINFDTLQKDARKYLIDMLVSNSLYEKAYDMAIEYGIDMLA
                     AASKVVLCENALKVQHVDDDFMVQLAISAFKTGKYSDLVLKYLCENYTGPTDELINLW
                     HAADKFSISSMKLDERILEQGIYTQIEPEKISDIFMEYYKRAGNEKLILAYISLVAHG
                     YLHSGGCKADFIFDIIEKRFIGNRTLNDACQLALLKHFAEKTDITQAELEIEDTLLKY
                     YIYNNMYFDFFARLDYRLLEKYFLYDKAFLQYESTPGTHVVLHYSRDEDGEEFNSEDM
                     VEMYDGIYVKTFVIFFGELIRYYITEEHDNSIEVKESNRLTCNNIPGDNDHSRYNLIN
                     EMIISDTLSDETTLKSNIDEYKRLDAATKQLFKLI"
     gene            7332..8420
                     /locus_tag="DHV81_05020"
     CDS             7332..8420
                     /locus_tag="DHV81_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase"
                     /protein_id="HCI93848.1"
                     /translation="MIEQKEHFHGSDLEKIEKIYGIKKENIVSFAANVNPLGESGKLK
                     TALSERIDAITKYPDREYTSLRKAIGSYCKCDYNHITVGNGCTELISLFIQITAPKKA
                     LLLGPTYSEYERDLRINGSDISYYFLKEKDDFKINSDEFISAITSDTDLVIICNPNNP
                     TGSLITPDKLKTILTHCKETNTYVMIDETYIEFVPDVDELSAIPLTELFDNVIILRGT
                     SKFFATPGLRLGYAITSNSQILTDINTNKNPWMINSLAVVAGETMFLDEEYIDKTRSL
                     ILSEKTRCRQLIEKSHKFKLYPSYSNFYLLKILDEGVDAHMLFERAIRQGMMIRDCST
                     FPGLEERFIRFCIMKPEDNTRLINCLTQ"
BASE COUNT         2434 a         2038 c         1450 g         2641 t
ORIGIN      
        1 tttgacaggt gcaagcagtg cttatcatat atagagattt cctttcctgc aacagttgtt
       61 ttaagactgt caagacgctc cttgtcacag aagcagtaat atgcctctcc cttgtcaata
      121 agttcctttg catacttcat gtagataccc tgtgccacac gctcactctg cacatatgga
      181 ccgcatccac catccttgtc aggtccctca tcatggacaa gtcctgtctc ttcgagtgtc
      241 ctgtttataa tatcaacagc accctccacc tggcgtacct gatctgtatc ctctatacgg
      301 agaatgaaat caccgccctc atgctttgca atcagataag catacagtgc tgttcttaaa
      361 tttcctacat gcattcttcc tgtcgggctt ggtgcaaatc ttgttctaat cttactcatt
      421 ttaaaatgtg ctcctctata ttaatatttt ttagtcattt ttcaatttga aattatataa
      481 caatatggaa attttatcaa tcctcagatt gaacttccat atcaagctct gcgattttat
      541 ttaaaattct gtttacctcg tggattctgt caccaagctc gctcctatgt gataaaatct
      601 ctcgtcttag cctggttata gtctccacat tgcaggacat cctgcccctt aatttttgtc
      661 tcctgactat aagttcatgc ttctgctcaa ccatctcctt gtgatccaca atagcgtttg
      721 tgtagttgag ccatactctg gcatcgtaga agtcattttt actaagcacc gaaacaagct
      781 ttttgctgtt gtattccaga tcctcgtttg tccttctgaa gcgctccttg tcttttacag
      841 cgagaagata ctgctcatag ttgtatacaa attcctttga attcttcaca tgatacttct
      901 cgtatgtgta gtcaacagca ttttttatac ttacatactt gatcttcact ctgttttcaa
      961 gagctactgc tttatttttg gatgctgcag cgctttttac ttcatctgaa tagctttgat
     1021 atgtcaaaag cacaaacgag cctgcaaccg ttgaaagaag tgccccgata agcataaaga
     1081 tagtggtatc ggctccatat acaatctgca tcagcgtgca tacagctacc actacagcaa
     1141 aaaataccac taaaagcact gcgtacaccc taaatgcccg ctgtctgctt ttggcatatt
     1201 caccatctat atcgaactgc actttttctc cctcgagata cctcaggtct ttatttatta
     1261 catccagact gctttcgttt ttttcaagac gctttattgc ccgcggaatc tcatcctcca
     1321 gctgctgcat ctgcgcatac tggctgtctg atatgcggtt tttggtcttt aaaaaatcac
     1381 ttctggtctt ttcgagtgtc aatatgtttg cagccgcatc attcatggtc tttttatctg
     1441 tctctgacat attttccatc ctttctatgt cagtcagata atttgtgaca agtgcatact
     1501 ctgttctgtt atcctcgagt tctctggcgg catcaaccat ctgttcacat atttcaacta
     1561 cattgaaccc tttagtactc tcatccggta tactctcaat atgtaattgt gtatcatcgg
     1621 tctttttttt gcgtttaaat aacatgcgcc ctgtaatcct ctttgtattt caaaattgtt
     1681 tcttttggtc tgccctcatc gcaaccatct gaagcttctg catatacgcc ctttaatacg
     1741 ctgtccagat tatttatttc ctgctctgta acactatatt ccctgacaat acgggcatat
     1801 cctatgtcgt tattatcaag cttataggca tatagacaag accacagtga ttttctgtcc
     1861 ccattttgcc tgaaagcctc aagatataaa tccgctgcta tatcaaacaa aaacagctgc
     1921 acataggctg tagccagatt atgatatatg tttccgtaca attcacgctc agtatgcttt
     1981 gcatcctcac atgacagcag ctttttgtat tcaaaaattg ctgcagtgta ctttttcttc
     2041 tccatcagtc tgtctgccct gattttcaag cactcaaact cgctcttttc ctcgagctcc
     2101 ttaagtgcat catgtgtgtt cttaagctcc tccttagtgc agtagccgct gtactcaagc
     2161 agctcagtca caaaagcaga cagtctgccg tttgtggata taatggttct aagatgcatg
     2221 gcaagctttg gcagatgtgc atggttttca atccaggtgc acagctcaag actcataaag
     2281 tcatcatcca aaagatatgt attgttgatt atataataat taagctcctc tatcgagtaa
     2341 acatttatag acacaccctc tatgtaatag ggcatggctg ctatagcttc attacacagt
     2401 aataattctc ccatatgccc tcctacatag tcatctcata gtgccagact ttgcccgagc
     2461 tcataaatat ttcgccaaag cctaaatcct taatctctat atctatcctg tcatcagata
     2521 caggtgtcgc cgtaattctg atgcgtgtag ccctatccgg cctgggcggc atatccgtaa
     2581 gtcttagtgt ctcaatcttt gcctccctgc ttttaggaag ctgcttccag aaatccacct
     2641 cataagagcc tgaaattata acctcacact gccccttgga ttcaaaccag ttgcttccgg
     2701 cactgataag attataaaac gacagttctc ccttatcatg taccttaagg ctcagattga
     2761 acttcatctc attctcgccc atatatacaa aaggccattc tgcttcccta tccctcgtgg
     2821 ctgccgcata gcatgccccc ttagtaaaga ggttattgcc catgaacact cgtcttccct
     2881 tgcacaaaag tgcaacagac tgtttcatcc agccctcagc aaacgtatct cccacgagaa
     2941 agaccgttga gataatctga ttcttaaagc tctcatttat tatatccaaa aagcttttgt
     3001 ccttatcatt tttatccaga gtatagctta tgctttcatc tatagatact acctgtggcg
     3061 ttgtgcgaag atccctcttt agggaataaa aaaatatact ttccttttcc tgcataaaca
     3121 tcaccacatc atgaagccac agtgacttgt cctggttcag tgcaaaataa taaaagctct
     3181 cctttcggtc tatgaccgta agctgtctgg aatttatgcc aagcctgacc gccatgccat
     3241 aaaacatgct cacgttctcc tttgtcagcc ggtcaacaca tataacaagc ctgtcaaagg
     3301 atgaaggatt accaagcttg gatggcagtt ccataacctt tttaagaaac aaggccaaaa
     3361 gctcctctgc cataaatgaa tcatcatcaa tgactatttt ttcactattc agtgctctct
     3421 taagcagctg gtcaatacac accatctcac cgcttttaga gagtcttctt gcctcatcac
     3481 cataatacca tttgccaatg ctcttacggc gtgcaagtac aagaggaatc tgaaactcct
     3541 cactgcccgc aacagtgctg acagtctccg gctctttcat attctgttga aaaaaactaa
     3601 ccatagcata gctgtcattt aaatctatgc ctatatagta tgaattgcca ttatgtccca
     3661 ttataaatcc tcttaaatca gcttaaacag ctgcttagtc gcagcatcca gccttttata
     3721 ttcgtctata ttgctcttta aggttgtctc atcagacagt gtatctgaaa ttatcatctc
     3781 attaatcaga ttatagcggc tgtgatcatt gtcacccggt atattgttgc acgtaagacg
     3841 attgctctct ttcacctcta tgctgttgtc atgctcctca gtaatataat acctgatcag
     3901 ctcaccgaaa aatatgacaa atgtctttac gtatatgccg tcgtacatct caaccatatc
     3961 ctctgaatta aactcttcgc catcctcgtc tcggctgtaa tgcagcacaa catgagtccc
     4021 cggagtactc tcatactgta aaaatgcctt gtcatagagg aaatacttct ccagcagtct
     4081 gtaatcaagt ctcgcaaaaa aatcaaaata catattgtta tatatataat atttaagcaa
     4141 tgtatcttca atctcaagtt cagcctgagt gatatctgtt ttctcagcaa aatgcttcaa
     4201 tagggctaac tggcaggcat catttaatgt tctgtttcca ataaatcgct tttcgataat
     4261 atcaaaaata aaatcagcct tgcacccgcc actatgcaga tatccatgtg ccacaagaga
     4321 tatataagct aaaatcagct tttcatttcc ggctctttta tagtattcca taaagatatc
     4381 cgatatcttt tcaggctcaa tctgcgtata aataccctgt tcgagaatac gctcatcgag
     4441 cttcatggaa gatattgaaa acttatcagc ggcatgccac aggttaatca gctcgtctgt
     4501 aggcccggta tagttttcac acaggtattt aagcaccagg tcactgtatt tgcctgtttt
     4561 aaaggcagat atggcaagct gaaccataaa atcatcatct acatgctgca ccttaagcgc
     4621 attctcacac aaaaccacct ttgatgcggc agcaagcatg tctattccat actcaatggc
     4681 catatcatat gccttttcat agaggctgtt tgatacaagc atgtcaataa gatattttct
     4741 tgcatccttt tgcagggtat caaaattaat gctttgcaaa aacggcctga cgtaatcctc
     4801 ataatcgtga agctgacaat attttaaaat tctgtatccc ataacagctt tgtaggaatc
     4861 agaaaaggat tcactataaa atacaggttt aaacagcttc aaatcatcct ttgtaaaatc
     4921 agaataatcc cttacatgct taaaatgtga cactatatac tgtggtctgt caggcgagag
     4981 gcttatacac ctgtcaaggt atttctcggc atccatcagc ctctgcagtc tgtatgagat
     5041 gcttttaaca tatctatagc cccttgagtc ctcaaaaagt ataacatagt cgtttgaaaa
     5101 cagttcaaaa taagcacact ggtctgtaac cggaactatc tgaggctcct tcatttcatt
     5161 ctgatatatg atagctctca ctatgcgctt atcaaataca ataagcttgt gagtatatat
     5221 aatatctgaa aaagctgccg acagctcttc attgataaat cccaactcaa gcatatcctc
     5281 gtataaaaca gccagattgt catcaatatg cctaagctgt gcctggtcca tggcaaacct
     5341 acccatagct ttcctgtact tgtggtagac ctcaggctct gtttccttgg cagcaattat
     5401 attgttgtaa agcactgcaa gctttcggta tggcagctta ttatcaaagc tgaaatacat
     5461 ctggataatc tttggcacag gggaaatctg cctgtcatcc atcgtaagga gatacgactc
     5521 atacaagcct gtcagacgca gcttattctc gatacccagc tcaaaccatt tatgaaaaca
     5581 ggtatcattt ttgtgtccct taatcaggta gctgcatatg atgccgacat actctgcctc
     5641 cggacatatc tcataggctg ctgtaagaag ctcatatact ctgttatcga acccgcctgc
     5701 cagatcaacc gcctcaaaaa ttgctccggc aagctccttt gtcagagcct tttcctttac
     5761 ggcccagctt aggatgcgaa gctcaaatac cgacagctcc tttatcagat aaggatcctg
     5821 tgatataaga taataagctt caatatacaa atacggagac gaacagcctc gcagtaccca
     5881 gtattttata tctttaagct taccggcact gtcatcaaaa tactgatccc tcaaaaataa
     5941 aagtacccag aacaaaagca cagagtcagg attttcataa aaaatcagct ccacttcatg
     6001 tgtcatattg tcaacgtatg aaggctctct ctctaacagc gtcatcagat acagataata
     6061 gccccatatt ggagctttct tatcaggatt actgtgcttg aaatcatcaa gaatccactt
     6121 agcttcctgc ctttgcctgt tgataataaa tgcctgagcc ttcatcagct catacatatg
     6181 ctcatccggc acgagtgcat gcaggtggtt taatatgctt atagtctcgt ttgcccatac
     6241 cccggtcact atccgcttca gcctgaaact ggtataaagc tccatgattc cgaccatgca
     6301 ttcctttatg tcctttgtca cagctatatc acttatgctg ccatcatcat ctcttacacc
     6361 ggccgtgata tctattgtaa agctctgata tacaccatca atatatatac ggccaaaatt
     6421 acgtccggca tgcattgcag atgcatcgat gatatactca tacagatagg ttttacctat
     6481 aaaatcatca agcgttaaaa caggcttaga cagctttatg aattcacaat ccgtgcgcag
     6541 gcgtatctcg gtatatcccc agttatcctt tgttatggta aagcatccac tctgtggctc
     6601 tttagaagcc ataaaaatct cctctgactt gtctacacta atggaaacag gctgtttttt
     6661 gtttacgcca atcagaaact cctccatatt ctgaccggat ggtctggcgc aggcaaagcc
     6721 ctcataggtc agtctctcat atacattgtc ataaggaatt gtgtttaaaa aattgcgatt
     6781 ataaaacaga tgataagcct catcccaatt agatgccgca agccttgtaa aatcatcaag
     6841 gctctttaca gctccggtgg atgcttccgc atagagcctt gtgattcttg cacaaaaaga
     6901 aagactgtac tctatctgat tgcatacaat cacaaatttg ccctcgcagg catcgccctc
     6961 cgtaaggccc ttactgttaa attgataacg aatcctgact ttctcgccct cgaagtgcgg
     7021 attcagacac tccatcctag gattagtcga ataaactatg ccacagattt ttatctgatt
     7081 cgtactttct attacaaagc ttcccgcttc gcttcttccc tcaatgaccg aaaggtctat
     7141 tgtctcctct gaaaacgcca aaatcggttt cgtgccatta aatctgccgg cagctatctg
     7201 ccctatacgt gctcgcacaa ttgttacccc gtttacgtta aatgttgatt ttttatacac
     7261 ttattatata atgtaaatta tatacgattt gcaatgctgt tgcaatgcaa atagaaagga
     7321 aattacagct aatgatagaa caaaaggagc attttcacgg cagtgacctt gaaaaaatag
     7381 aaaaaatata cggtataaag aaagaaaata ttgtgagctt tgcagcgaat gtcaacccac
     7441 tcggtgagtc cggtaaacta aagactgcac tgtctgagcg cattgatgca atcacaaaat
     7501 accctgacag ggagtatact tctctcagaa aggctatcgg cagctactgc aagtgcgatt
     7561 acaatcacat aacagtcgga aacggatgca cggagctcat ctctcttttc attcagatta
     7621 cagctcctaa aaaggcactt ctgcttggtc cgacttactc tgagtacgag cgtgatttaa
     7681 ggataaacgg cagtgatatc tcatactatt ttcttaagga aaaggatgat tttaagatta
     7741 attcggatga attcatctct gcaatcacat ctgataccga ccttgtcatc atctgtaatc
     7801 ccaacaatcc gaccggcagt cttatcacac cggataagct caaaacgatt ttaacccact
     7861 gtaaagagac aaatacttat gtgatgatag acgagacata tatagagttt gtgccggatg
     7921 ttgatgagct ttcagcaata cctctgactg aattgtttga caatgtgatt attttacgcg
     7981 gcacatccaa gttttttgcc actccgggac ttcgtctggg ctatgccatc acatcaaatt
     8041 cacaaattct tacagacata aacacaaaca aaaatccgtg gatgattaac tctctcgccg
     8101 ttgttgcagg cgaaaccatg tttttggatg aagaatatat agataaaaca agaagcttga
     8161 tactttcgga gaaaaccaga tgcaggcaat tgattgaaaa atcacacaaa ttcaagcttt
     8221 atccatccta cagcaacttc tatctgctta agatacttga tgaaggcgtg gatgcacata
     8281 tgctatttga gcgcgccata aggcagggca tgatgataag ggactgctct accttccccg
     8341 ggcttgagga gcggttcata cgattttgta taatgaagcc agaggacaac acaaggctta
     8401 ttaattgcct tacacaatga tgctaaatgc ctttatataa acataacata taaccaaaaa
     8461 gcctccggaa ggatttaatc cagccggagg cttttttata aagtgaaaca tagaatatgc
     8521 tttaaggtct atattatttt gcagataaga actcgtcaat acc
//
LOCUS       DPUF01000144           15193 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13630, whole
            genome shotgun sequence.
ACCESSION   DPUF01000144 DPUF01000000
VERSION     DPUF01000144.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 15193)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15193)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15193
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13630"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..>267
                     /locus_tag="DHV81_05025"
     CDS             <1..>267
                     /locus_tag="DHV81_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCI93849.1"
                     /translation="SNLITGGIIWDIIFGSIATLLGALGTYFLRKKKFVYTLPPVIAN
                     IIIVPLVLRYGYGFTTVYKGVDISLLFNAVTVGIGEIISICVLGS"
     assembly_gap    268..396
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(480..2087)
                     /locus_tag="DHV81_05030"
     CDS             complement(480..2087)
                     /locus_tag="DHV81_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold/beta-CASP domain-containing RNA
                     metallo-hydrolase"
                     /protein_id="HCI93850.1"
                     /translation="MVLEFIGADREVTGSCHYLTFGDTHILIDCGMEQGADLYVNQEI
                     PVNPSLIDYVFVTHAHIDHSGLLPLIYNHGFRGTIFATKATTDLCNIMLKDSAHIQEF
                     EAEWKNRKARRAGRPEVTPMYNVEDAQNVMQHFTPCDYNSVYEICPGLKVRFVDAGHL
                     LGSSSIEMWVTEENVTKKIVFSGDIGNHNRPLIKDPQYVDSADYVVMESTYGNRLHDT
                     PPDYAVELSKVINATFTRGGNLVIPAFSVGRTQEMLYFIRRIKTENLLPEHPNFEVYI
                     DSPLAVEATNVFHENVSDCFDEEAMELISAGINPIKFPGLRVAVSSDESKMINFDKKP
                     KVIISASGMCEAGRIRHHLKHNLWRSDSTVLFVGYQVPGTLGYALLNGAKKVKLFGEE
                     IEVRASIVNLPGISGHADKNQLTEWLGAIKNKPEHVFIVHGEESTAESFANHVHETFG
                     YDAVAPYSGDAYDLITNQKVADGSRKLVEKKAAYGMASREKTIFDRLVAAGQRLVSVI
                     QKCQGMANKDLSKFADQINALCDKWDR"
     gene            complement(2133..2867)
                     /locus_tag="DHV81_05035"
     CDS             complement(2133..2867)
                     /locus_tag="DHV81_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HCI93851.1"
                     /translation="MKLFLVIKRVWKILWGSKPHTNEVFYIGGTEVLPPPLETDEEKC
                     CLIALVDKNVAMSKEQAKKQLIEHNLRLVVYLAKKFENTGVCVEDLISIGTIGLIKGI
                     NTFNPEKKIKLATYASRCIENEILMYLRRNNRVKLEVSFDEPLNTDWDGNELLLSDIL
                     GTDEDVIYKDLETEVEISLLKKAMLSLNERERQIIELRFGVNRVENKELTQKEVADIL
                     GISQSYISRLEKKIMARLKKEIQKMA"
     gene            complement(2800..3630)
                     /locus_tag="DHV81_05040"
     CDS             complement(2800..3630)
                     /locus_tag="DHV81_05040"
                     /inference="COORDINATES: protein motif:HMM:PF03419.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93852.1"
                     /translation="MIYAFYLDIFLLRTFITNCLILIFVIYMLKIPLIGKVVRLIASA
                     MGASVCECVLLLLAGSYAFYKWTSVLVVVPLAARLAVGRQAGRKIPFLRLFALCMFVT
                     VLTGGIVTALDSFFSSDATVMICFGFVISYIVLKCFCERSNESRRLYRVTLLKDGKRA
                     CATALYDTGNLLKKQPQQIPVHIGGSALFDIVGEDVSFFDVPYKSLGNDGGSIKVCEF
                     DEMTVMKGNSKLILHNVLVGRASDSLFEDNAYDMILNEAVFSNKTGMENTLGKQAAHK
                     "
     gene            complement(3744..5000)
                     /locus_tag="DHV81_05045"
     CDS             complement(3744..5000)
                     /locus_tag="DHV81_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCI93853.1"
                     /translation="MLEIRSNEADNSAKIIVIGVGGAGNNAVNRMIDENIGGVEFIGI
                     NTDKQALQLCKAPTLIQIGEKLTKGLGAGAQPEIGQKAAEESAEELQAAVKGADMVFV
                     TCGMGGGTGTGAAPVVAKIAKDQGILTVGVVTKPFKFEAKQRMINAVSGIERLKESVD
                     TLIVIPNDKLLEIVDRRTTMPDALKKADEVLQQAVQGITDLINLPALINLDFADVQTV
                     MKDKGMAHIGIGSAQGDDKAIEAVKLAVASPLLETKINGATHVIINISGDISLMDAND
                     AASYVQDLAGENANIIFGAKFDESMTDQASITVIATGLEDVSEKIDMPGKQAAPGAGM
                     AGGMQNRMVYPNQTAARPVSGMGTQSTATAGLHTTATSGLHTAAQPQQTAPAHAYTGI
                     QKPRQPESTVKPVEINIPDFLKNSRR"
     gene            complement(5144..5887)
                     /locus_tag="DHV81_05050"
     CDS             complement(5144..5887)
                     /locus_tag="DHV81_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCI93854.1"
                     /translation="MIKKKRKHKKRLKHFFMGLLITIAVLAILFLIALKLFTVKRVVV
                     EGNELYDQKTIEDAVLNDKYSWNSLYVYLKYKVKDTKKIPFIDTMSISLDSPHTLHIS
                     VYEKGMLGYIYIPGINENAYFDKDGFVVETSSDTVPGVPCIDGISCDKVVLYEKLPIK
                     QTMLKDILELTQGLKRQDLVPDSVDYGAQNAPVAKYGNVSVIIGDTTKLTRKIERLKA
                     IMPSLEGQSGTLHLESWTEETTNIVFDKS"
     gene            complement(5906..7051)
                     /gene="ftsW"
                     /locus_tag="DHV81_05055"
     CDS             complement(5906..7051)
                     /gene="ftsW"
                     /locus_tag="DHV81_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HCI93855.1"
                     /translation="MAGKRRRSSKNKEKKEKGIPYFDYNLLFIVIFLLCFGLVMLYSS
                     SAYTSAIKNHDSMHYLKLQIRNIVLGLIPMVFLAKVDYRYWKKLGFFAYIASLILCVL
                     VFVPKIGSSSHGSSRWIGIGPIQFQPSEVAKIAVILFMAMIIDKIPKQFDKFLSLVKV
                     LAMLIPLIIVVAISNLSTAVIIIGISVCMLFVASPKYLQFIIVAVAVVVFAVAFVMLA
                     GYRSTRIEAWLHPETAGTDAVFQTMMGLYAIGSGGLFGKGLGESLQKLGNVPESQNDM
                     IFTIICEELGLFGAICLILLFILLIWRMMVIANNARDLYGSLLVIGVMSHIAIQVILN
                     IAVVTNSLPNTGVILPFISYGGTSIIFLMAEIGLVLSVSRGIRLENV"
     gene            complement(7057..8418)
                     /gene="murD"
                     /locus_tag="DHV81_05060"
     CDS             complement(7057..8418)
                     /gene="murD"
                     /locus_tag="DHV81_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCI93856.1"
                     /translation="MSLQGKKVLVVGTGKSGIAATELLCANGIDTVLFDGNKELDSKT
                     LYEKAPKLKDVPLILGDLTDEQIDEFDVAVLSPGVPTDIPMVNSLRDRGVKIWGEIEL
                     AYTFGAGEIIAITGTNGKTTTTALTGEIMKNYFKDVRVVGNIGIPYTSMVTGSTGETV
                     TVAEISSFQLETIDTFKPHVSAILNITPDHLNRHHTMENYIRAKEDITKNQTADDYCV
                     LNYEDEVLRDFAAECPAKVIFFSSKSELSEGFYLDGDIIIYAHDGVRDEVIDVNELNL
                     LGKHNFENVMAACAMSISFGVPMDKIVEVLKVFKAVEHRIEYVTEKRGVRFYNDSKGT
                     NPDAAIQGIRAMNRPTLLIGGGYDKQSEYDEWIEAFDGKVKELVLIGQTADKIEECAH
                     RHGFMNTVKKDTFEDAVNYCYEHAVSGDAVLLSPACASWGMFPNYEERGRIFKEIVKG
                     FKE"
     gene            complement(8549..10240)
                     /locus_tag="DHV81_05065"
     CDS             complement(8549..10240)
                     /locus_tag="DHV81_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase"
                     /protein_id="HCI93857.1"
                     /translation="MILNNTRYRRKICVFCSILTAVLIALCFRLFYLMIVKGDYYSKK
                     ASALQERERDIAGIRGDIVDRNERIIATNETAYNISVIHNQITDKEAVISVLSSELSI
                     DEKTIRSKVDKVTSIEKIKNNVSKKTGDKILSYGLAGVKVDESSVRYYPLNELFSKVI
                     GFAGADGQGVVGLETTYDEILRGTPGRIYTVCDGRGVEVKGYKERREAPQKGDTLVTT
                     LDINIQRVCEKNAMMAYAQNLADSVSIIALNPKNGEIYAMTDYPEYNLNDPFSCENHD
                     EAWRNGCVSDTYEPGSVFKIITAGIALEEDVVSLDDRFYCPGYITVEDRRIHCHKRTG
                     HGSETFVQGIQNSCNPVFIDLGLRIGSERYYADFKRFGLLDKTGIDLPGEASTIMHQP
                     DKIGQVELATISFGQSFQLTPIELMTTVSSLINGGNRITPHIGKEIHGTEGTVKEVLS
                     FEQTSGICSEETSDTLRKLLEGVVAEGSGKNAKVEGYAIGGKTATSQTLPRSDNVYIA
                     SFLGFYPVDDPALMVLVKINNPKGGAYYGGTIAAPVAAEIFREIIPYAQEIGVCN"
     gene            complement(10300..12213)
                     /locus_tag="DHV81_05070"
     CDS             complement(10300..12213)
                     /locus_tag="DHV81_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase"
                     /protein_id="HCI93858.1"
                     /translation="MKTKLFALFMLIVVAMLGLIGRLMFIEYTSGDAYTKKVLSLQSY
                     DSKTIPFQRGNIVDSNGTVLATSVAVYNVVLDCSVMTSKKEYITPTIDALTQCFPDLD
                     RATLEDYANNSKDNKYIVLLKKLSYDAIQPFVQLQDATDEKGNLKNPNIKGVWFETEY
                     QRNYPFKTLASSVIGFTSAGNVGTTGLENYYNDTLNGVNGREYGYLNADNDFQKTVIA
                     AQNGNTLYTTIDANIQSIVEDKIKLFSDTYANNAREGLGAENIAVIIENPKNGEILAM
                     ANYPNFDLNNPRDMSAYYSEEQLAAFKKDSTQEMDALNKLWQNFCVTHTYEPGSVQKP
                     FTVACGLDTGTLTTDMTFLCDGYEMFGSEKVSCVNRSGHGIETLEKALMDSCNDALMQ
                     MSYKIGAENFLYYQSVFGFGQKTGVDLPGEANTSTLMYTLDNIKPVDLATNVFGQNYN
                     CTMIEMVSAFSSLVNGGNYYQPHVVKKIADDNGNTVKTIEPTLLKKTVSENTSATLKN
                     YLQNVVANGTGKVAKVDGYSMGGKTGTAQMYDETTHLRKRGSYLVSFIGCVPAQDPQL
                     VIYTVIDQPNVQDQPHSNYAQNLTREILKEVLPYMNIYPDEEQTGINADLTITGTNPP
                     TGEKVTQEGEQGE"
     gene            complement(12277..12753)
                     /locus_tag="DHV81_05075"
     CDS             complement(12277..12753)
                     /locus_tag="DHV81_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93859.1"
                     /translation="MNKTTYYINGNAVRELDEAAPVRRPQKSTRELEEIRRKKNRRTS
                     ARRNQERALSMNRAFVAFLTACVAASALVSVSLIQIRSNVTQQMKEVAALESRINDMK
                     ADNDARYKQITTSVDLNAIKDAAINRLGMKYASQDQIVYYSIDKNNYMDQYSDIPE"
     gene            complement(12778..13722)
                     /locus_tag="DHV81_05080"
     CDS             complement(12778..13722)
                     /locus_tag="DHV81_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HCI93860.1"
                     /translation="MEFKHYSVLRDETIENLNIRPDGIYVDGTLGGAGHSYEIAKRLS
                     DKGRLIGIDQDADAIKAAGERLAEFGDRVTIVRSNYSDMKNVLHSLGIEKVDGIMLDL
                     GVSSFQLDTPKRGFTYRSEDAPLDMRMDDRNSLTARDIVNTYSENDLYRIIRDYGEDK
                     FAKNIAKHIVAARQKQEITTTGELNEIIKAAIPQKVRATGGHPSKRTYQAIRIELNHE
                     LDVLRDNLDDMIDLLNPGGRICIITFHSLEDRIVKSNFKKNENPCTCPPSFPVCVCGN
                     KSKGTVITRKPILPSEKELEENSRSKSAKLRVFEKNEL"
     gene            complement(13742..14173)
                     /locus_tag="DHV81_05085"
     CDS             complement(13742..14173)
                     /locus_tag="DHV81_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MraZ"
                     /protein_id="HCI93861.1"
                     /translation="MFMGEYNHSIDAKGRMIVPAKFREQLGNEFVVTKGLDGCLFVYS
                     NEEWHRIEENLRDKPLTSREARKFMRFFFAGAATCEVDKQGRILLPANLREYAGIDKE
                     VVSVGVYSRVEIWSKDRYLENSDFDDMDEIADHMAELGIGI"
     gene            complement(14387..>15193)
                     /gene="lgt"
                     /locus_tag="DHV81_05090"
     CDS             complement(14387..>15193)
                     /gene="lgt"
                     /locus_tag="DHV81_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCI93862.1"
                     /translation="GIIIACGMLAGLGVACYVAKKSGQNPDTYFDLALVAIICSVIGA
                     RIYYVVFRWDLYKDDLLSIFNIRQGGLAIYGGVIAAIITTFVFAKVRKLSFPLLCDTA
                     GLGLILGQAIGRWGNFCNREAFGDYYDGLFAMQLPVSAVRTSDLTEKLMSHTTVVDGV
                     EYISVHPTFLYESFWNLCLFILLMLYFKHRRFDGEVFLLYLLGYGIGRFWIESLRTDQ
                     LLIPHINYPVSMALAATLVAVSAIWIGIVRYKQMKMGKMVDTPPQSTTKE"
BASE COUNT         3927 a         3787 c         2600 g         4750 t
ORIGIN      
        1 tccaatctta tcacaggcgg cataatctgg gatatcatct tcggaagtat cgctacactg
       61 ctcggtgcac ttggcactta cttcctcaga aaaaagaagt ttgtatacac gcttccacct
      121 gtcatcgcca acattattat cgtgccactc gtgctcagat acggatatgg ttttacaact
      181 gtatacaagg gtgttgatat ctctcttctt ttcaatgcag ttacagtcgg aatcggtgag
      241 attatctcaa tctgtgtact tggaagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaaa ataaaaagct ctgtgtcata
      421 cacttatatg tatgcacaga gctttttact ttattacttt aatatgctgt gattacattc
      481 tatctatccc acttgtcaca cagcgcattt atctggtctg caaacttgct caaatcctta
      541 tttgccatac cctgacactt ctggataaca gatacaagcc tctggcctgc tgccacaaga
      601 cggtcaaata tagtcttctc tctgcttgcc atgccatatg cagccttttt ctccacaagc
      661 ttgcgggagc catctgccac cttctgattc gtgataaggt catatgcatc accactgtac
      721 ggtgccactg catcatagcc gaaggtctca tgcacgtggt ttgcaaagga ctctgctgtg
      781 gactcctctc catggactat aaatacatgc tccggcttgt tttttattgc gccaagccac
      841 tcggtaagct gatttttgtc tgcatgtcca ctgatacccg gcagatttac tatgcttgcc
      901 cttacttcta tttcctcacc aaaaagcttc acctttttag caccgtttaa aagtgcatag
      961 ccaagcgtgc ccggaacctg atatcctaca aacaaaactg ttgaatcact tctccaaaga
     1021 ttatgcttta agtgatgcct gatacgtccg gcctcacaca tacccgatgc tgatattatg
     1081 accttcggct tcttatcgaa attaatcatc tttgactcat ccgatgatac tgccacacga
     1141 agtccgggga acttgattgg attgatgcct gccgaaataa gctccatagc ctcctcatca
     1201 aagcagtccg atacattctc atgaaataca tttgtcgcct caacagcaag ggggctatcg
     1261 atgtaaacct caaaattcgg atgctccggc aacagattct cagtctttat acgcctgata
     1321 aagtagagca tctcctgtgt cctgccaact gaaaatgccg ggattaccag atttccgcct
     1381 cttgtaaatg tcgcattgat gacctttgaa agctccaccg cgtagtcagg cggcgtatca
     1441 tgcagacgat tgccatatgt agattccata accacataat cagcactgtc cacatactgc
     1501 ggatccttga tgagcggcct gttgtgattt ccgatatcac ctgaaaagac aatctttttg
     1561 gtcacattct cctcagtcac ccacatctca atggatgatg aaccaagcag atgacctgca
     1621 tccacaaatc tcaccttaag ccccgggcat atctcataca cactgttgta atcacacggt
     1681 gtaaagtgct gcatgacatt ctgtgcatcc tccacattgt acataggagt cacttccggt
     1741 cttccagctc gtcttgcctt tctgttcttc cactccgcct caaattcctg aatatgtgca
     1801 gaatccttta gcatgatgtt acataaatct gttgtcgcct ttgtcgcaaa aatggtgccc
     1861 ctgaaaccat gattgtatat gagtggcaaa agacctgagt ggtcaatgtg tgcgtgtgtg
     1921 acaaacacat agtctatgag tgaaggattg accggtatct cctgattcac gtacaaatca
     1981 gctccctgct ccattccaca gtcaataagt atatgtgtgt ccccaaatgt cagatagtga
     2041 cagcttccag taacctctct gtcagcccca ataaattcta ataccatact accatctcct
     2101 attctgtttt ataatagaat attataccac acttatgcca ttttctgtat ttctttcttc
     2161 aatcttgcca ttattttttt ttcgagcctt gatatgtatg actgtgaaat tcccagaata
     2221 tctgccacct ccttctgcgt cagttcctta ttctccacac ggtttactcc aaatcgaagc
     2281 tctattatct gcctttcacg ctcgttcaat gacagcatgg ctttttttag cagtgaaatc
     2341 tcaacctcag tctcaagatc cttatatatg acatcctcat ccgtccccag tatatctgat
     2401 aaaagcagct catttccgtc ccaatccgtg ttcagtggct catcaaagga tacctcaagc
     2461 ttcactctgt tgttgcggcg cagatacata agaatctcat tttctatgca tctggatgcg
     2521 tatgtggcaa gctttatttt cttctcagga ttgaatgtgt ttatcccctt tatgagaccg
     2581 attgtgccaa ttgatataag gtcctccacg catactcccg tgttttcaaa tttttttgcc
     2641 agatacacca caagccttag attgtgctct ataagctgct tttttgcctg ttcctttgac
     2701 attgccacat ttttatccac cagtgcaatc agacagcact tctcctcatc tgtctcaagc
     2761 ggaggcggca gtacctccgt ccctcctatg taaaacacct catttgtgtg cggcttgctt
     2821 ccccagagta ttttccatac ccgttttatt actaaaaaca gcttcattca aaatcatatc
     2881 atatgcattg tcctcaaata aactatccga tgctcttccc accagcacat tatgtaaaat
     2941 cagcttacta tttcccttca tgactgtcat ctcatcaaat tcacacactt taatgcttcc
     3001 accatcattt cccagactct tatacggcac atcaaaaaag ctgacatctt ctcctacaat
     3061 atcaaacaat gcactcccgc ctatgtgcac cggaatctgc tgtggctgtt tttttagcag
     3121 atttcccgta tcatacaagg ctgtggcaca agctctttta ccatccttta aaagtgtgac
     3181 cctgtatagc cttctgctct cattacttcg ctcacagaag cactttaaca ctatgtatga
     3241 tatgacaaag ccaaagcata tcataactgt cgcatcagag ctgaaaaagc tgtccagagc
     3301 tgttactatg cctccggtca gcactgttac aaacatacat agtgcaaaaa gtcttagaaa
     3361 cgggattttt ctgccagcct gtctgcctac tgcaagcctt gccgcaagcg gtacgaccac
     3421 cagaacagag gtccatttat aaaacgcata gctgcctgcc aaaagaagca gtacacattc
     3481 acataccgat gcacccatcg cagaagcaat cagacgtacc accttcccga tcaacgggat
     3541 ttttagcata tatatgacaa atataagtat gagacagttg gtgataaatg tacgaagaag
     3601 aaaaatatcg agataaaatg catagataat ttttcacctc actttcacag acaagaatag
     3661 cgtaaaagca ggtttttaag tgccgtaata tgtcgacaat aagtaaaaaa gacatgcagc
     3721 ccaaaagctg catgtcctat aaactatcgt cttgaattct taaggaaatc cggaatatta
     3781 atctctacag gcttaaccgt gctctcaggc tggcgtggct tctggatacc tgtatatgca
     3841 tgtgcaggtg ctgtctgctg tggctgggca gctgtatgaa gtccgcttgt tgctgttgtg
     3901 tgaagccctg ctgttgccgt ggattgtgtt cccatgcctg aaacaggtct tgcagctgtc
     3961 tgattcggat ataccatacg gttttgcata ccgcctgcca ttcctgcacc aggcgctgcc
     4021 tgcttgcccg gcatatctat cttctctgaa acatcctcca gtcctgtagc aataacagtg
     4081 atagaagcct gatcagtcat gctctcatca aacttggcac caaagataat attggcattc
     4141 tctcctgcca gatcctgtac gtagcttgct gcatcgtttg catccataag agagatatct
     4201 cctgagatat tgataatgac atgtgttgca ccatttatct ttgtctcaag aagaggtgaa
     4261 gcaaccgcaa gcttaacagc ttcgatagcc ttatcatcac cctgtgcact accgataccg
     4321 atatgtgcca tacccttgtc cttcatgaca gtctgtacat ctgcgaagtc aaggttgata
     4381 agtgcaggaa gattaataag atcagtgatt ccctgaactg cctgctgcaa tacctcatct
     4441 gccttcttga gtgcatccgg cattgttgta cgacggtcta ctatctcaag aagcttatca
     4501 tttggaatga caataagtgt atcaacgctc tccttaagtc tctctattcc tgaaacagca
     4561 ttaatcattc gctgctttgc ctcaaatttg aatggctttg taacaacacc aactgtaagt
     4621 atgccctgat cctttgctat ctttgcaaca acaggagctg cacctgtacc ggttccgcct
     4681 cccataccac atgtgacgaa taccatatca gcgcccttta ctgctgcctg aagctcctct
     4741 gcgctctcct cagcggcctt ctgaccgatc tcaggctgcg cacctgcgcc gagtcccttt
     4801 gtaagcttct ctccaatctg tataagtgta ggagccttac aaagctgtaa agcctgctta
     4861 tctgtattga taccgataaa ctctactcca ccaatattct catcaatcat tctgttaact
     4921 gcattgttac cggctcctcc gactccaatg acaataatct ttgcgctgtt atcagcctcg
     4981 ttacttctta tctcaagcaa ggtgattcct cctctttctt tcccaaaatt taattttcta
     5041 tataaaaata tcgaatattc ttaaactcat ttattctata atataatttt tctccaaaat
     5101 aatcaactat aaaatatgat tttttttatt tagttttaca ttattatgac ttgtcaaaca
     5161 caatatttgt ggtttcttcc gtccagctct ctaaatgtag tgtaccggac tgtccctcaa
     5221 gcgatggcat gatagcctta agcctctcta ttttacgtgt aagctttgtc gtgtcaccaa
     5281 ttatcacact cacattacca tacttggcta ccggtgcatt ttgcgcacca taatccacag
     5341 aatccggcac aaggtcctgc ctttttaatc cctgggtaag ctcaaggata tcttttagca
     5401 tagtctgctt tataggcagc ttctcataaa gcactacctt gtcacagctt atgccgtcta
     5461 tacatggcac acccggcacc gtgtctgagg atgtttccac cacaaagcca tccttatcaa
     5521 aatatgcatt ctcattaatt cccggaatat atatgtatcc aagcattcct ttttcataaa
     5581 ctgatatatg aagagtatgc ggactatcca ggcttatgct cattgtatca ataaacggta
     5641 tttttttagt atcctttacc ttatatttca gatagacata cagtgagttc catgagtact
     5701 tatcattaag caccgcatcc tctatagtct tttggtcgta aagctcatta ccctcgacaa
     5761 ctaccctttt taccgtaaac agcttaagtg caatgagaaa cagtatcgcg agcactgcta
     5821 tcgtaattag cagtcccata aaaaaatgct ttaatctctt tttatgtttt ctttttttct
     5881 taatcattat atttcacgcc ttaatctaca cattttcaag cctgatgcct cttgacacag
     5941 acaaaacaag ccctatttcc gccatcagga atatgattga cgtacctcca tagctgataa
     6001 acggcagaat aacaccggta ttgggaagtg aattggtaac taccgcaata ttcagtatca
     6061 cctggattgc aatatgactc atcacaccaa tcactaaaag tgagccatac agatcccttg
     6121 cattgtttgc aatgaccatc atacgccata taagcagtat aaaaagtaaa ataaggcaga
     6181 tagctccaaa caatccaagt tcttcacaaa taattgtaaa tatcatatca ttttgtgact
     6241 ccggcacatt gccaagcttc tgtaagctct ctccaagtcc ttttccaaac aatccgcccg
     6301 agcctatcgc ataaagtccc atcatagtct ggaatactgc gtctgttccc gcagtctccg
     6361 gatgcagcca tgcctcaata cgtgtacttc tatagcctgc cagcatgaca aacgccactg
     6421 caaacacaac taccgccact gctacaataa taaactgcag atactttggg cttgcgacaa
     6481 acaacatgca gaccgaaatg cctatgatga taactgccgt actcagattg ctgattgcaa
     6541 caactattat gagcggaata agcatagcaa gcacctttac cagacttaaa aatttatcaa
     6601 actgcttggg tatcttgtct ataatcatag ccataaacag tatgaccgca attttagcca
     6661 cttctgaagg ctggaactgt attggtccta tgccaatcca tctcgatgaa ccatgtgagc
     6721 ttgagcctat tttcggcaca aacacaagca cacacagtat aagcgatgct atatacgcaa
     6781 aaaagccaag ctttttccaa tatctgtagt cgacctttgc cagaaatacc ataggtataa
     6841 gtcccagtac aatatttcgt atctgaagct taagatagtg catgctgtca tgatttttga
     6901 tggccgatgt gtatgcactc gagctatata gcataacaag accaaagcag agcagaaata
     6961 taactataaa caacaggttg tagtcgaaat aaggtattcc tttttctttt ttttctttat
     7021 tcttgctact tcttctacgc tttcctgcca aaatatttac tccttaaatc cttttactat
     7081 ttccttaaag atacgtccgc gctcctcata gttaggaaac atgccccagc ttgcacatgc
     7141 aggtgataag agcactgcat cacctgaaac agcatgctca tagcaataat ttactgcatc
     7201 ctcaaacgta tccttcttta cagtattcat gaatccgtgt ctgtgtgcac actcttcgat
     7261 tttatctgca gtctgaccga taagcacaag ctcctttact ttgccgtcaa aggcctctat
     7321 ccactcatca tactccgact gcttatcata tccaccgcca atgagaagtg tcggtctgtt
     7381 catggcacgt ataccctgaa tggctgcatc aggattggtg ccctttgaat cgttgtagaa
     7441 tctgactccc cgcttttcgg tcacgtactc tatcctgtgc tcaactgcct taaatacctt
     7501 aagcacctcg acaatcttat ccatcggtac tccaaagctt atagacatgg cacacgctgc
     7561 cataacattc tcaaaattat gctttccaag gagattcagc tcgtttacat caattacctc
     7621 atcgcgtaca ccgtcgtgtg cgtatatgat gatgtctccg tccagataaa agccctctga
     7681 aagttcagac ttactgctga aaaatataac cttagccggg cactctgctg caaaatctct
     7741 aagcacctcg tcctcgtagt ttaacacgca gtaatcatct gctgtctgat tttttgtgat
     7801 atcctccttt gccctgatgt aattttccat agtatggtgt cgattcaggt gatcgggtgt
     7861 gatatttaaa attgcactta catgtggctt aaaggtatct attgtctcga gctggaagct
     7921 tgatatctct gcaaccgtta ctgtctcacc ggtgcttcct gtgaccattg atgtataagg
     7981 gattccgata tttcctacca ctctaacatc cttaaagtaa ttcttcatta tctcacctgt
     8041 cagggctgta gtggtcgtct ttccgttggt tcctgtgata gcgatgatct cacctgcccc
     8101 aaatgtatat gcaagctcta tctcacccca tattttaacg cccctgtcac gtaaggaatt
     8161 gaccatagga atgtctgttg gtactcccgg acttagcaca gccacatcaa actcatcaat
     8221 ctgttcatct gtcaggtcgc ctaaaataag cggcacatcc ttaagctttg gtgctttctc
     8281 ataaagtgtt tttgaatcca gttctttatt tccatcaaac aatactgtat cgatgccatt
     8341 tgcgcacaaa agctctgtcg ctgcaatgcc actctttcct gttccgacaa caagtacctt
     8401 ttttccttgt aagctcattt ttattcctcc atcttttatt gcagccatcc gtatggatat
     8461 gtttaacaaa tcaatttctc caaacgactg ccttaatcat taaattatag tatacgttat
     8521 ttagttctat ttcgcaagag cccgtaaatc agttacacac tcctatctcc tgtgcatacg
     8581 gtatgatttc cctaaatatt tcagctgcaa ccggtgcagc gattgttccg ccatagtatg
     8641 caccgccctt tggattattt attttcacaa gcaccataag tgccggatca tcaaccggat
     8701 aaaacccaag gaacgaggct atatatacat tgtcacttct cggaagtgtc tgcgatgtag
     8761 cagtctttcc tcctatcgca tacccttcaa cctttgcatt ttttcctgag ccctctgcta
     8821 ccacgccctc caaaagcttt cgcagtgtat cagaggtttc ttcactgcat atacctgacg
     8881 tctgttcaaa ggacagcacc tcttttaccg tgccttcagt gccatgaatc tccttaccta
     8941 tatgaggagt aattctgtta ccaccattta taagcgatga tactgttgtc ataagctcga
     9001 tgggtgtgag ctggaatgac tgtccaaatg aaattgttgc aagctccacc tggcctatct
     9061 tatccggctg atgcattatg gtggaggcct ctcccggcaa atcaatacct gtcttatcaa
     9121 gcagaccaaa cctcttgaaa tcagcataat acctctcact gcctattcta aggcctaaat
     9181 caataaagac cggattgcat gaattctgta ttccctgcac aaaggtctca cttccatgtc
     9241 ctgttctctt atggcagtgt atgcgtctgt cctccacagt aatatatccc gggcagtaaa
     9301 agcggtcatc aaggctcacc acatcctcct ccagtgcgat tcctgccgta attatcttga
     9361 atactgaacc gggctcatat gtatcactta cacatccgtt tctccaggct tcatcatggt
     9421 tttcacatga aaaaggatca ttcaaattat attccgggta gtcggtcatg gcatatattt
     9481 ctccattttt agggtttaag gcaattatac tcacactgtc tgccagattt tgtgcatatg
     9541 ccatcattgc atttttctca catactcttt gtatatttat gtcgagtgta gtgacaagtg
     9601 tgtctccttt ttgtggagct tctcttctct ccttgtagcc cttcacctcc acacctctgc
     9661 catcacatac agtgtatatc cgcccgggtg tgccacgcag aatttcatca tatgtggttt
     9721 caagcccaac aacaccctga ccatctgctc ctgcaaagcc tataaccttt gaaaacaact
     9781 cattaagcgg atagtacctg acacttgact cgtcaacctt tacccctgcc aagccatatg
     9841 atagtatttt atcgcctgtt tttttagaga cattgttctt tattttttca atggatgtaa
     9901 ccttatccac cttactgcgg atagtctttt cgtcaatcga aagctcactg cttaaaacag
     9961 agatgactgc ttccttgtcg gttatctgat tgtgtataac gcttatgtta tatgcagttt
    10021 catttgtcgc tattatcctt tcatttctat ccactatatc tccgcgtatt cctgcaatat
    10081 cacgctctct ttcctgaagc gcacttgcct ttttactgta ataatcgcct tttactatca
    10141 tgagataaaa cagcctgaaa cacaaggcga taagaacagc tgtcagtata ctgcaaaata
    10201 cgcatatttt tcttctgtag cgcgtattgt taagtatcat aaacaaaaat gcctcaatat
    10261 ttttacacta atatatgcaa aaaatattga ggctattctc tattcgccct gctcaccctc
    10321 ctgtgtgacc ttttctcctg tcggaggatt tgtgcctgtt atcgtaaggt cagcatttat
    10381 accggtctgt tcctcatcag ggtatatatt catatacgga agtacttcct ttaatatctc
    10441 tctggtcagg ttctgtgcat aattactgtg aggctgatcc tgtacattcg gctggtcgat
    10501 tacagtgtaa ataacaagct gcggatcctg cgcaggcaca cagcctataa atgatacaag
    10561 atagctgccc ctctttctaa ggtgcgttgt ctcatcatac atctgtgctg ttcctgtctt
    10621 gcctcccatt gagtatccgt caacctttgc caccttaccg gttccgtttg caactacgtt
    10681 ctgcaggtag tttttaagtg tcgcacttgt attctctgac actgtctttt ttagcagtgt
    10741 aggctcaatt gtctttactg tattgccgtt gtcatctgcg attttcttta ctacgtgcgg
    10801 ctgataataa ttgccgccat tcaccagtga agaaaatgca ctgaccatct ctatcatggt
    10861 acagttatag ttctgtccga atacgtttgt cgctaaatcg acaggcttta tattgtcgag
    10921 tgtatacatg agcgttgatg tatttgcctc acccggcagg tcaacacctg tcttttgacc
    10981 aaagccaaat acactctgat aatacagaaa attttctgca ccaatcttat atgacatctg
    11041 catgagtgca tcattacatg aatccatgag tgccttttca agtgtctcta tgccatgtcc
    11101 gctacggttg acgcatgata ccttttcact gccaaacatc tcatatccat cacataaaaa
    11161 tgtcatatca gtagtgagcg tgcctgtatc cagtccacat gccacggtaa aaggcttctg
    11221 tacagacccc ggctcatatg tatgtgtaac acagaagttc tgccacagct tatttagtgc
    11281 atccatctcc tgagtactgt cctttttaaa tgccgcaagc tgttcctcac tgtaatatgc
    11341 actcatatcc cttggattat tcaaatcaaa attcggataa ttcgccattg ccagtatctc
    11401 accattttta ggattttcta taatgactgc aatattttct gcgccaagtc cttctcttgc
    11461 attatttgca taagtatctg agaacagttt tatcttatcc tcaacaatcg actggatatt
    11521 tgcgtctata gttgtataca gggtatttcc gttttgtgca gcaattactg tcttctggaa
    11581 atcattgtct gcattcagat agccgtactc cctgccattt acaccattta aggtgtcatt
    11641 gtaatagttt tcgagtccgg ttgtacctac atttccggcg gaggtaaagc cgataactga
    11701 gcttgccagt gttttaaacg gataatttct ctgatactct gtctcaaacc acacaccctt
    11761 gatgtttggg ttcttcagat tgcccttttc gtctgttgca tcctgcagct gtacaaatgg
    11821 ctgaattgca tcatatgaca gcttttttaa cagtacaata tatttgttat ccttgctgtt
    11881 atttgcataa tcctcaagtg ttgccctgtc taaatccgga aagcactgtg tcagtgcatc
    11941 tatcgtcgga gttatatact ctttcttact ggtcataacc gagcaatcaa gtacaacatt
    12001 ataaacagcc acactcgtag caagcactgt gccgtttgaa tctacaatat ttcctctctg
    12061 aaacggtatt gttttgctgt catatgactg taatgatagt acctttttag tatatgcgtc
    12121 tccgcttgta tattcaataa acatcagcct gccaatgagt ccaagcatgg ctacaactat
    12181 taacataaac aacgcaaaca gctttgtttt cattttgcgt ccgatttttc ttaccggtct
    12241 ttttcttgtc ctggctgcca atatgattcc tccaatttat tcagggatat cactgtactg
    12301 atccatatag ttgttcttat caattgagta ataaactatc tgatcctgtg aagcatattt
    12361 cataccaagt ctgttgatag ctgcatcctt gattgcattt aaatcgacag atgtagtaat
    12421 ctgcttgtat ctggcatcat tgtcagcctt catatcattt atccggctct caagtgctgc
    12481 aacctccttc atctgctgtg tcacatttga ccttatctga ataagtgaaa cagatacaag
    12541 ggctgaagct gccacacacg ctgtgagaaa ggctacaaat gccctgttca ttgagagtgc
    12601 tctctcctga tttcttcttg cagatgttct tctgtttttc tttcttctta tttcctcaag
    12661 ctctcttgtc gatttctgtg gtcttctgac cggtgctgcc tcgtcaagct ctcttacggc
    12721 attcccattt atataataag tagttttatt catttagctg ctccttttgc tgtcattcta
    12781 caattcattc ttttcaaaca cacgaagctt tgcactcttc gagcgtgaat tctcttcaag
    12841 ttccttttct gacggaagga taggttttct tgtgataacc gttcccttcg atttattacc
    12901 gcatacacat accggaaaac tcggtggaca tgtacatgga ttttcgtttt ttttgaaatt
    12961 ggattttact attctgtcct caagcgagtg gaatgtgatt atacatatcc tgccgcccgg
    13021 attaagtaaa tctatcatgt catccaggtt gtcccttagc acatcaagct catgattcag
    13081 ctcaattctg atagcctgat atgttctctt cgacgggtga ccaccggtag ctctgacctt
    13141 ttggggtatc gctgccttta ttatctcgtt gagctctcct gttgtcgtta tctcttgctt
    13201 ttgtcttgct gctacaatgt gctttgcaat attctttgca aacttgtcct caccgtaatc
    13261 cctgatgata cggtacagat cattttcgct gtaggtattt actatatccc ttgcagtaag
    13321 ggaattcctg tcatccatac gcatatcaag cggtgcatcc tcactcctgt aggtaaagcc
    13381 tctctttggt gtatcaagct ggaaggatga cactccaaga tccagcataa taccatccac
    13441 cttttcaatt ccgagtgaat gaagcacatt cttcatatca gaataattgc ttctgactat
    13501 cgttactctg tctccaaact ccgcaagtct ctctcctgcc gcttttatgg catctgcatc
    13561 ctgatctatg cctatcagcc tgcccttgtc cgaaagtctc ttggctatct catatgagtg
    13621 cccggctcct ccgagagtac catccacata tatcccgtca ggacggatgt ttaaattttc
    13681 aattgtctcg tcccttaaga ctgaataatg cttaaattcc atgtggtctc tccatttaat
    13741 atcaaatacc tataccaagc tctgccatat gatctgctat ctcatccata tcgtcaaaat
    13801 cagagttctc aaggtatctg tccttgctcc agatttctac tctgctgtat actccgacag
    13861 atactacttc cttatctata cctgcgtatt ctcttaagtt cgccggtaag aggattctgc
    13921 cctgcttgtc cacctcacag gtggccgctc ctgcaaagaa gaatctcata aattttcttg
    13981 cttctcttga tgtgagtggt ttgtcgcgaa gattttcctc aatgcgatgc cattcttcgt
    14041 tggaatagac gaacagacag ccatctaagc ccttggtaac tacaaactca ttaccaagct
    14101 gttcacggaa cttcgccggg acaatcatac ggcccttggc gtctattgaa tgattatatt
    14161 cgcccatgaa catggcatcc atcctccttt tcctcatttt gaagctttcc accacttcac
    14221 tccacttata tggtattttg ctccactttc caccacttga atacaatatt acacctaaca
    14281 gcctacaaaa tcaaggcttt aagccaaatt cccataaaaa agcaacaaaa aagacgctca
    14341 gtcttttcgt ggactttgcg tcttttttat aaaatacata tgaaatttac tctttagtgg
    14401 tggattgtgg tggagtgtcc accattttac ccattttcat ctgcttatat ctgacaatac
    14461 ctatccatat ggctgatact gccaccaaag tggcggcaag tgccatagac accggataat
    14521 tgatatgtgg gatgagaagc tggtctgtac gcaggctttc tatccagaat cttccgatac
    14581 catagcctaa caaatataga agaaacacct ctccgtcaaa ccttctgtgc ttaaaataaa
    14641 gcatcaacaa aataaataag cacaggttcc aaaaggattc atacaaaaat gtaggatgca
    14701 ccgagatata ctccactccg tctaccacag ttgtatgtga cataagcttt tcagtcagat
    14761 ccgatgtcct gacagctgat accggaagct gcatggcaaa aagtccgtca taataatcgc
    14821 caaatgcctc cctgttgcag aagtttcccc atcggccaat cgcctgtccc agtatcagtc
    14881 caaggcccgc cgtatcacac aacagtggaa aagacagctt tctgaccttt gcaaaaacga
    14941 atgtggtgat aattgctgct atcactcctc cgtaaattgc cagtcctccc tgcctgatat
    15001 tgaatatgct aagcaggtcg tccttgtaca aatcccacct gaataccaca tagtatattc
    15061 ttgcgcctat caccgagcaa atgattgcca caagtgccaa atcaaagtat gtatccggat
    15121 tttgtcctga ttttttagcc acatagcagg ccacgcccag ccccgcaagc ataccacatg
    15181 ctattattat gcc
//
LOCUS       DPUF01000145           21446 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_2949, whole
            genome shotgun sequence.
ACCESSION   DPUF01000145 DPUF01000000
VERSION     DPUF01000145.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 21446)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21446)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21446
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_2949"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="DHV81_05095"
     CDS             <1..441
                     /locus_tag="DHV81_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCI93863.1"
                     /translation="VKELKGKKLAVVPILRAGLGMVEGMLELIPAAKVGHIGMYRDPE
                     TAEPIEYYCKLPADCANREVFVVDPMLATGGSAVAALDMLKKRGVKNIHFMCIIAAPE
                     GVKRLTEAHPDVDVYIGALDDHLNEHKYIVPGLGDAGDRIFGTK"
     gene            547..>1031
                     /locus_tag="DHV81_05100"
     CDS             547..>1031
                     /locus_tag="DHV81_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCI93864.1"
                     /translation="MGASEKRKDYISWDEYFMGIAMLSGMRSKDPNTQVGACIVDPEH
                     KILSMGYNGFPLGCSDDEFTWAREGEDNKYFYSTHSELNAILNYRGGSLEGATIYVTL
                     FPCNECAKAIIQSGIREVVYDCDKYEGTASVTASKRMLRAAGVTIRRYEHTDREVTLV
                     L"
     assembly_gap    1032..1093
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1222..1521
                     /locus_tag="DHV81_05105"
     CDS             1222..1521
                     /locus_tag="DHV81_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATPase subunit"
                     /protein_id="HCI93865.1"
                     /translation="MNALIMVFQFGINMIVPIFLCTLAGVWIGKKTGADWVTVPLFFL
                     GAFAGANNIYRMVQKFLKPEDDGRAVRQTPGRQSTTETEISDSISRVEKDVKKNK"
     gene            1502..1933
                     /locus_tag="DHV81_05110"
     CDS             1502..1933
                     /locus_tag="DHV81_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93866.1"
                     /translation="MLKRINDALPGLVFGIVFYGIVVQLIGVWFVTDKISYSIGLWYG
                     IAIAVGIAVNIATVIYDATAIYGQKDANRRIVAKSLLRYIVVAVLLFILGLFDFGNLI
                     MAFVGMLGLKISAYLQPLFMKVTDRFSGRSDASSAGEMETE"
     gene            1973..2710
                     /gene="atpB"
                     /locus_tag="DHV81_05115"
     CDS             1973..2710
                     /gene="atpB"
                     /locus_tag="DHV81_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit A"
                     /protein_id="HCI93867.1"
                     /translation="MNQAILLESNADNIDFMIHGIFSIKVFGQEVWITTSHACILIVM
                     VIMVIFAIVANRKLKKAKEVPDGFQNIIELIVEMLDKMVEGTMGKWAPRFVNYISTIF
                     IFILMSNISGLFGLRPPTADYGTTLALALITFFMIRFNKAKHQSAKDIWTGMCSPLPP
                     WLPIWAPINIISEIAIPISLSLRLFANVLSGTVIMALVYGLLRFIAIAWPSVLHVYFD
                     LFSGAIQTYVFCMLTMTYVTQACETDE"
     gene            2744..2998
                     /gene="atpE"
                     /locus_tag="DHV81_05120"
     CDS             2744..2998
                     /gene="atpE"
                     /locus_tag="DHV81_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCI93868.1"
                     /translation="MDINGQDLIRACSAIGAGLAVIAGIGPGVGQGIAAGHGAAAVGR
                     NPGARGQIMSTMLLGQAVAETTGLYGLVVALLLLFVHPLG"
     gene            3064..3594
                     /gene="atpF"
                     /locus_tag="DHV81_05125"
     CDS             3064..3594
                     /gene="atpF"
                     /locus_tag="DHV81_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HCI93869.1"
                     /translation="MLAQGYTFLFNLDAQLLFDTVLLAVAVFFLFMLMSYLLFNPARK
                     LLKDRQDRIESDINTAVADKESAAALKAEYEGKLKDIDKEAEAILTEARQKALKNQNK
                     IVDDAKEEASRIIKRAREEAELEKKHAMDDMKQEMIQIASLMAQKAVAASITTDIQDT
                     LVEETLKEMGESTWLS"
     gene            3582..4175
                     /gene="atpH"
                     /locus_tag="DHV81_05130"
                     /pseudo
     CDS             3582..4175
                     /gene="atpH"
                     /locus_tag="DHV81_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741047.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
     assembly_gap    3803..3884
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4172..5677
                     /locus_tag="DHV81_05135"
     CDS             4172..5677
                     /locus_tag="DHV81_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCI93870.1"
                     /translation="MNLKPEEISSVIKEQIKRYASQLEVADVGTVIQVADGIARIHGL
                     ENAMQGELLEFPGEVYGMVLNLEEDNVGAVLLGQQRNINEGDTVKTTGRVVEVPVGDA
                     MLGRVVNALGQPIDGKGPIQSDKYRQIERVASGVISRKSVDTPLQTGIKAIDSMVPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKGQGVKCIYVAIGQKASTVASIVNELEEFGAM
                     SYTTVVAATASELAPLQYIAPYSGCAIGEEWMENGQDVLIVYDDLSKHAAAYRTLSLL
                     LKRAPGREAYPGDVFYLHSRLLERAARLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLETEMFNAGFRPAINAGLSVSRVGGSAQIKAMKKIAAPIRVELAQYREL
                     AAFSQFGSELDASTAEQLAQGARIKEILKQPQYKPMPVEKQVIIIYAATKKYLIDIKI
                     EDILRFEAELFDFIDTRYPEIPEAIREKKVMDDDTEQALIKAIEECKKQFR"
     gene            5711..6571
                     /gene="atpG"
                     /locus_tag="DHV81_05140"
     CDS             5711..6571
                     /gene="atpG"
                     /locus_tag="DHV81_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HCI93871.1"
                     /translation="MASMRDIKRRKSSITSTQQITKAMKLVSTVKLQKARAHAEATDP
                     YFNYMYRTVSSMLAKSGNLEHPYLKAGDSPRKAVVVLTSNRGLAGGYNSNIVKLITHG
                     DFKKEELDIYAVGAKGEELLTSKGYHIVESASEMMEDPTYEDAAALCKRVLKAFTDGQ
                     VGEIYLAYTHFKNTVTQEAKLIKLLPVELDESGADEKEDSNILMNFEPNPEEALELII
                     PKYVTSLFYGALVESFASENGARMQAMDSATSNAEDMISDLTLKYNRARQGSITQELT
                     EIIAGANAIE"
     gene            6671..8062
                     /gene="atpD"
                     /locus_tag="DHV81_05145"
     CDS             6671..8062
                     /gene="atpD"
                     /locus_tag="DHV81_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCI93872.1"
                     /translation="MADKNVGKIIQVISAVLDIKFSEGNLPEINDAVEVPLKNGGKLV
                     VEVAQHLGDDTVRCIAMGPTDGLVRGMDAIATGAPISVPVGENTLGRMFNVLGEPIDE
                     VEPPQTEEKWAIHRPAPSFEEQATSQEMLETGIKVVDLLCPYQKGGKIGLFGGAGVGK
                     TVLIQELIHNIATQHGGYSVFTGVGERTREGNDLYYEMKESGVIDKTTMVFGQMNEPP
                     GARMRVALAGLTMAEYFRDRGGKDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPT
                     LQTEMGALQERITSTKNGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLERSIAEL
                     GIYPAVDPLASTSRILDPRIVGQEHFEVAHAVQEILQKYKELQDIIAILGMDELSEED
                     KLVVSRARKVQRFLSQPFFVAGQFTGLEGKYVPIAETIRGFKEILEGKHDDVPESYFL
                     NVGSIDEVRAKMQ"
     gene            8077..8484
                     /gene="atpC"
                     /locus_tag="DHV81_05150"
     CDS             8077..8484
                     /gene="atpC"
                     /locus_tag="DHV81_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HCI93873.1"
                     /translation="MADNIFDIEIITPDRVFYKGETDFLEFNTQNGEIGVYKNHIPLT
                     TVLAPGIVTIHNGDEEKIAAIHSGFAEILGDKVTFLAELAEWPDEIDIDRAQAAKERA
                     EQRLSNHTAEIDVKRAEFALRKALIRIDAAHHV"
     gene            8856..9866
                     /locus_tag="DHV81_05155"
     CDS             8856..9866
                     /locus_tag="DHV81_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HCI93874.1"
                     /translation="MIKFDEVEYRGNLTKQIAAINKGYEYAKEITADGYSNIFFVAVG
                     GTMAMMLHFAAIAKQKTKIPVYVEQAAELVVAGNTQLNKDSLVIMGSKSGDTKETVAA
                     AKWCKERGIRIASMVIDKESPLGQLTDYQLPLVVFKGVEYEYLSMFGLFYGLLNCNGD
                     FPEYDQFVEALAKLPDTLVDAQKKFDEPADAIAKKYFNADYMMWIGSGELWGEVYLFS
                     MCILEEMQWIRTKSITSPEFFHGTLELVEKDMPVFLVKSAGVYRTLDERAEKFLKEHT
                     DNLIVIDVMDYMIPGLKPEFAPLVAPIISTAVLNGRLSKHIESYTGHDLDMRRYYRQF
                     EY"
     gene            9876..10526
                     /locus_tag="DHV81_05160"
     CDS             9876..10526
                     /locus_tag="DHV81_05160"
                     /inference="COORDINATES: protein motif:HMM:PF13419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCI93875.1"
                     /translation="MIEAVLFDMDGILIDSEREYDKAMREAITRYGHMITDEFLIRVR
                     GIPVEAFKKSLKLEFGRDFPVEKFRNDFKSIVWQNIRQYGMPVKNGVYQLIQYLEVNN
                     IRYAVATSNDRAMALELLEAADLRGVFEYMVCGDEVEHGKPFPDIYQKAADMLGVNIK
                     HTLVIEDSYNGIRAGHAAGASVIMVPDLVQPTDEIRQMTDYVVSDLGKVISCISNF"
     gene            complement(10513..11241)
                     /locus_tag="DHV81_05165"
     CDS             complement(10513..11241)
                     /locus_tag="DHV81_05165"
                     /inference="COORDINATES: protein motif:HMM:PF07702.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93876.1"
                     /translation="MNHYLHVKADDAAEAIENYISMHNLNPHDRIPSERAMAEELGIN
                     RTTCREALKRLENEHILYSIVGSGTYVAPPKLRTSIGMGFSLDAYCEVNGFKRSNKLI
                     YCYQTLATDYISKILGLSPYSPIYIIKRLRFINQKPAMIETTHLSEAMFPELLRKMKE
                     NETQSLYALMKNDYNIIPTHHSYNVSMNICDIDNAVLLGIETNSPLLNFSIISKDSNN
                     NVIEYCQSLRRIDRCCINASFKNC"
     gene            complement(11251..11946)
                     /locus_tag="DHV81_05170"
     CDS             complement(11251..11946)
                     /locus_tag="DHV81_05170"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00392.19,HMM:PF07702.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI93877.1"
                     /translation="MDSHSSKYIYDTLKGDIENNLYSFGSALPSERTLAEKYFVSRTT
                     VRNAIEQLVHDGLLYKIQGKGTYVSMPKLHATNSVTSTRKYLKDHHLKPSTKIITSGI
                     RNAGYKYSNIFNISESDQLFFVYRQRLGNKIPYSVEYTFLPFAYVPNAQSYNFAKESL
                     YDCFNDNNIIVDHTVQTIDLVKIFKPQSDILQQPDGSASFKRVNTVYDIHNRVVEYTL
                     SYSLADKFKFVFD"
     gene            12047..12466
                     /locus_tag="DHV81_05175"
     CDS             12047..12466
                     /locus_tag="DHV81_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCI93878.1"
                     /translation="MVKIMLASHGNLAAGMLSSAEMVFGKQDNVSVICAYVDGEDDVS
                     TRIKGFIDSIAPDDSWIIFTDLFGGSVNNEFMKYISNPQIRLIAGMNLALVITAMSIV
                     EMKNNAAEVEEELLGIADSVINFCSRTGIDSLEDDEF"
     gene            12484..12954
                     /locus_tag="DHV81_05180"
     CDS             12484..12954
                     /locus_tag="DHV81_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIB"
                     /protein_id="HCI93879.1"
                     /translation="MIVNVRVDHRLLHGQVAMAWNQVLGSDCILIANDSVPNDEIRKA
                     GIKLAKPANTKLVIKNMDASVEALVSGVTDKYKLFIVVESIADVYKLCKETGMIDKIT
                     LGGVKSREGTRQISKAVFTTPKEEELIKELITDGVTVEIRQVPSDSVIYAKDVL"
     gene            12966..13742
                     /locus_tag="DHV81_05185"
     CDS             12966..13742
                     /locus_tag="DHV81_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
                     /protein_id="HCI93880.1"
                     /translation="MILKAILLGLVAMLGHTNFLFGTNLLDRPLIMCTLTGLVMGDLK
                     SGIIIGAMMELAFIGAFSVGASLPPDMISGGVLGAALTLAAGNDPEVALTIGVPIASL
                     ALLMKNACKIFILPIFVHKADDYAVKGNSKGVARMHMLGGFLYLNLPYGIFVFSAFLL
                     GNTVIQSVLDAIPQFIKSGLTIATGLMPALGFAVLASMIINKKNWYFLLLGFLISAYM
                     GMSVTGVALFATVIALIIVNMQNNQVITATEMGGDEDDDF"
     gene            13729..14550
                     /locus_tag="DHV81_05190"
     CDS             13729..14550
                     /locus_tag="DHV81_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCI93881.1"
                     /translation="MTTSKLITKRDLRKVFFRSFPMEWTWNYEKQQNLGYTYAMIPII
                     EKLYKDKPEEKAAALQRHLEFFNTTPHMVTLILGISTAMEEENALDENFDTSSINSVK
                     AGLMGPLAGIGDSFMWGTLRIIATGIGTSLAMNGNILGPILFFLLFNIPHVILRYICM
                     MGGYKFGAGFLSKIAAGGLMEKLTYAASILGLMSIGSMIATMVTVNIPLTYTNGDTSV
                     VIADVINGIVPCLLPLLFTGFIYWLVTKKKMKTTWALILIFIIGILGSLTGILGV"
     gene            14627..15739
                     /locus_tag="DHV81_05195"
     CDS             14627..15739
                     /locus_tag="DHV81_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HCI93882.1"
                     /translation="MAKTMFDYINSSTELIEENLKKRIALTETMVNEYIKGPYHNITI
                     VASGSSNNAAQCARLYMEKVLKCKVTVISPFEFIHAEHSVASDEMVIAVSQSGYSVNT
                     MEALKFINDELTRKSIVLTGDLNSDIVSVADLAVDFGVGEETVGYVTKGVIALSLYLM
                     LFALEAAKMCKIIGQNEYDTWVREFEKVSGAHEKMIVATKKFYADNIKALTSLQTVYI
                     CSVGANMGTAMEGALKVGETIQVPGIAYELEEYIHGPNLQLTPNYTVFFIDGGLASDR
                     TQEIFEATGVITDKTYMLTNKKGLEGKNIINPDFEINELITPLAFLPFFQILSYQITE
                     DTHKWKKHPLMDEMKRLAGAKTLNYESSPLRRDMPQ"
     gene            15902..17008
                     /locus_tag="DHV81_05200"
                     /pseudo
     CDS             15902..17008
                     /locus_tag="DHV81_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     assembly_gap    17130..17213
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17290..17521
                     /locus_tag="DHV81_05205"
                     /pseudo
     CDS             17290..17521
                     /locus_tag="DHV81_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17702..18532)
                     /locus_tag="DHV81_05210"
     CDS             complement(17702..18532)
                     /locus_tag="DHV81_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI93883.1"
                     /translation="MSNRKFNLDNEVQPEIHFRLLSISASKFENDWTSFPHSHHFTEI
                     FFIKDGTGRMLIENESLPVSSNSLVVIGAQVTHTEFSDPENPLDYYVLGVEGLKLNTD
                     KEPEYIHVPASANSPFIRQCFENILREMHNKQDRYAEICQHYLDILILYFCRKDHVSY
                     EVVDAQNSSRECHKVKRFIEHNYQSMISLDSLAENCSLSKYYLSHRFAELYGKSPIAY
                     LNEVRLASARDLLLTTNHSIEEIAGCIGFSSTSYFSQSFQKNFHESPQQFRKSHRVRT
                     "
     gene            18770..20116
                     /locus_tag="DHV81_05215"
     CDS             18770..20116
                     /locus_tag="DHV81_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI93884.1"
                     /translation="MKKKILCTMISMVMVASLFAGCGTDSGKENSASDTSKTKEAGTT
                     ISVAAIETAYGSEMWQKVADAFTEETGIKVELTTDKKLEDVIGPSMQGGEYPDVIHLA
                     TGREAALTEQFIKGHMITDITDVLSMKVPGEDKLVSEKIAGGFTDTSLTNPYGDGKTY
                     LAPMFYSPCGLFYNAGLLEEKGWDVPKTWDEMWELGDKAKEEGIYLFTYPTTGYFDAF
                     FYALMYSAGGPEFFDKATNYAEGIWETPEAQTCFDIVAKLAEYTNPVTPAQANDQDFT
                     QNQQLVLDNKAIFMPNGTWIVGEMAEAPRADGFKWGMTALPAVKDGGDAYSYTWFEQA
                     WIPSGAEHQDAAKLFISYLYSDKACEIFAKAGAIQPVLGIADKLSGDNVMFYSIYDNG
                     AKAAMGNFAAFEAIPGVEVRTVFFDPVNSLVSGDMDEQAWIDGIISASDQMRANLK"
     gene            20225..21109
                     /locus_tag="DHV81_05220"
     CDS             20225..21109
                     /locus_tag="DHV81_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI93885.1"
                     /translation="MKKARGRSGFLAVCIAPAVILFFVFMILPTLNVFRMSLFEKGAY
                     SPNETFVGLKNFKTLISDTNFIRSMQNMILLIVVVTIVTFAFALVFAGILSREQIRGQ
                     NFFRIIFYIPNILSVVVIAGIFSAIYKPENGMLNSIIGLFHKMENPILWKGESLVIVS
                     LIIAMIWQAIGYYMVMYMASMASVPKSLYESAGLDGAGRLVQFFQITIPLIWTNIRTT
                     LTFFIISTINMAFLFVKAMTSGGPNGASEVSLSYMYGQKDAGLYGYSMAIGVVIFIFS
                     FALSALVNKVTEREPLEF"
     gene            21121..>21446
                     /locus_tag="DHV81_05225"
     CDS             21121..>21446
                     /locus_tag="DHV81_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI93886.1"
                     /translation="MREKKNPNSAERLYKFFIYLVLIMLAVSIIIPVAWVFMASIKQN
                     KEFYGNPWTLPEGFYFQNFIDAWNTAKMGDYMLNSVLVTALALVILLIVALPAAYCLS
                     RFKFKG"
BASE COUNT         6757 a         3492 c         5125 g         5844 t
ORIGIN      
        1 gtaaaggaat taaagggcaa aaagcttgca gttgttccta tcttaagagc cggacttggt
       61 atggtggagg gcatgcttga gctcatccct gccgcaaagg ttggacacat cggcatgtat
      121 cgtgacccgg agacagcaga gcctatcgag tactattgca agcttccggc agactgcgca
      181 aacagagagg tatttgtggt agatccgatg cttgcaacag gcggttcagc agtggcagca
      241 cttgatatgc tcaaaaagcg cggtgtgaaa aacatccact ttatgtgcat aattgcagct
      301 cctgagggag taaagagact cacagaggca catcctgatg tggatgtata tataggagcg
      361 ctcgatgatc atctcaatga gcacaaatat attgtgccgg gactcggaga tgcaggagac
      421 agaattttcg gaaccaagta ataataatga tgataatagc tgcaggttgc tctgaggtgt
      481 aaacagtgat ataaaacagt aaagtaacag acagtgtgat agaccatatt gaatggaggt
      541 gtatatatgg gagcttcgga aaagagaaag gattatatct catgggatga gtattttatg
      601 ggcatagcga tgctttcagg tatgcgctcg aaggatccga atacccaggt gggtgcgtgc
      661 atagtggacc cggagcacaa gatactctcc atgggctata acggctttcc tcttggctgc
      721 tcagatgatg agttcacctg ggccagagag ggagaggata acaagtattt ctattctaca
      781 cacagcgagc tcaatgccat cttaaactac aggggcggaa gccttgaggg agcgaccata
      841 tatgtcaccc tgtttccgtg caatgagtgt gctaaggcaa tcatacagtc cggcatcagg
      901 gaagtagtct acgactgtga caaatacgag ggcacggcca gcgtgacagc ttcaaagagg
      961 atgcttaggg cagcgggggt tacaattcgc aggtatgagc acactgaccg tgaggtgacg
     1021 cttgtgctgt annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnntgtcaaa ttgacgtgat ttgacatgaa acatatgtat tttttatgca
     1141 ttttacacat aaaaatgtag acaaaagcaa taaaaatgat ataataattt gtatatgaaa
     1201 agagataata agaaagaggt aatgaatgca cttatcatgg ttttccagtt tggaatcaac
     1261 atgattgtgc cgatatttct ctgcacactg gccggtgtgt ggattgggaa aaagaccgga
     1321 gcggactggg taactgttcc attgtttttc cttggagcat ttgcgggggc aaacaatata
     1381 taccgcatgg ttcaaaaatt cctgaagcca gaggatgatg gcagagccgt cagacagaca
     1441 ccgggcaggc agagtactac ggaaacagag atatcagaca gtattagcag ggtagagaag
     1501 gatgttaaaa agaataaatg acgcattgcc gggacttgta tttgggatag ttttttacgg
     1561 gattgttgtg cagcttattg gggtatggtt tgtcacagac aaaatttcat attctatcgg
     1621 cttatggtat gggatagcga tagctgttgg tatagctgtc aatatcgcca cagtcatata
     1681 tgacgcgacc gccatatatg gacaaaagga tgccaatcgc aggatcgtag caaaatcact
     1741 tttaaggtat attgttgttg cggtcctgtt atttatattg ggactatttg attttggaaa
     1801 tctgataatg gcctttgtcg gtatgctggg actcaagatt tcagcgtatt tacagccttt
     1861 atttatgaag gtgacagaca gattttccgg gagaagtgat gcatcttccg ccggcgaaat
     1921 ggagacagag tagcaaaaca gcaggtaaac ttaataagga ggaggtgata atgtgaatca
     1981 ggcgatttta ctggaaagta acgcagacaa tatcgacttt atgatccatg gtattttttc
     2041 gataaaggta ttcggtcagg aggtctggat taccacatcg catgcctgta tcctcatagt
     2101 gatggtcatt atggtcatat tcgctatagt ggcaaacagg aagcttaaaa aagcaaagga
     2161 agtaccggac ggctttcaga atatcatcga gctcatcgta gagatgctcg acaagatggt
     2221 cgagggaacc atgggtaagt gggctccgag gtttgtaaac tacatcagca cgatttttat
     2281 atttatactg atgagtaata tctcaggctt gtttggttta agaccaccga cagccgacta
     2341 cggaaccaca ctggcgcttg cacttattac atttttcatg atcagattca ataaggctaa
     2401 gcatcaaagc gcaaaggata tctggaccgg tatgtgttca ccactgccgc cgtggctgcc
     2461 tatatgggcg ccaatcaaca ttatcagtga gattgcaatt cctatctcat tatcattgcg
     2521 tttgttcgcc aatgtgttgt caggaactgt tatcatggcg ctggtatatg gtctgctaag
     2581 gtttatagcc atagcgtggc cgtcagtact gcatgtatac tttgatttgt tctcaggagc
     2641 aattcagaca tatgtattct gtatgctgac aatgacatac gttacacagg catgtgagac
     2701 ggatgagtaa aactataaga tgtattttta ggaggattca attatggata tcaatggaca
     2761 ggatttaatc agagcatgct cagcaatcgg tgcaggtttg gcagttatcg ccggaatcgg
     2821 acctggtgtt ggacagggta tcgcagcagg acatggtgca gcagccgtag gacgtaaccc
     2881 gggagcaaga ggacagatta tgtcaaccat gcttttagga caggcggttg cagagacaac
     2941 aggtctttat ggtctcgttg ttgcattact tttacttttc gttcatccgc tcggatagta
     3001 tattacggtt tgccgcagag gattaccgtg cggcgataca gccaacggaa aggagagtaa
     3061 aagttgttag cacagggata tacatttttg tttaatctgg atgcgcagtt gctctttgat
     3121 acagtacttt tggcagttgc agtatttttc cttttcatgc tgatgtcgta tctgcttttc
     3181 aaccctgcaa ggaagctgtt aaaggacagg caggacagga tagaaagcga tataaataca
     3241 gctgttgccg acaaggaaag tgccgcagca ctaaaggcag agtacgaggg aaagcttaag
     3301 gacatcgata aagaggctga ggctattctc acagaggcga gacagaaggc gcttaaaaac
     3361 cagaacaaga ttgtggatga tgcaaaggaa gaggcttcac gcatcataaa gagagccaga
     3421 gaagaggcag agcttgagaa gaagcatgcc atggatgata tgaagcagga gatgatacag
     3481 atagcatcgc ttatggcaca aaaggctgtc gcagcttcaa tcaccacaga cattcaggat
     3541 acactcgttg aagagacact taaggaaatg ggagaatcaa catggctaag ctagtatcta
     3601 aggtatacgg tgatgcattg tttgaggctg cccttgagga caacaggcta tccggcctgt
     3661 ctgacgaggt aacagcagta aaggaaatca ttgctgcaaa tgatgacctc tcaaagctga
     3721 tggatcatcc gcagattgat aaagaagaaa aggtcagaat catcgaagaa atatttgggg
     3781 gaagagtttc agcggagctg gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttcgac tactttcttg
     3901 acagagtcaa ggagtaccaa aatatcggaa cagcatacgt cacatctgca tttgagcttt
     3961 caaaggagca gaagcttgct gtggagaaac gacttcttga cacgacaaaa tacgtgaaat
     4021 ttgagatgca ttttgaggtg gataccgcgc tcataggcgg tatgaaaatc agaatcgggg
     4081 acagagtggt ggattcatct gtcagcagca gattagaaag actcacccgc gatttgacaa
     4141 aaatacaatt gaaagtaggt gaatgcgctc catgaatcta aagccggaag aaatcagctc
     4201 agtaataaag gagcagatta aaagatacgc ttcccagctc gaagtcgctg atgtaggaac
     4261 agtcattcag gtggcagacg gaatcgcacg tattcatggt cttgagaatg cgatgcaggg
     4321 agagcttttg gaatttccag gggaagtata tggaatggta ttaaacctcg aggaggacaa
     4381 tgtcggtgct gttttacttg gacagcagcg caatatcaac gagggagata cagttaagac
     4441 cacaggacga gtggtagagg tgccggtagg cgatgcgatg ctcggccgag tagtaaatgc
     4501 gctcggacag ccaatcgacg gaaagggacc tatccagtcg gacaaatatc gtcagataga
     4561 gagagtagca tcgggagtta tctcgagaaa gtcagtagat acaccgcttc agaccggtat
     4621 aaaggctatt gactcaatgg ttcctatcgg acgaggacaa agagagctta ttatcggaga
     4681 cagacagacc ggaaagacgg ctatcgcgat agataccata atcaaccaga agggacaggg
     4741 tgtgaaatgt atctatgtag ccatcggtca gaaggcttct acggttgcat ccatagtaaa
     4801 tgagctcgag gagttcggag ccatgagcta cacgacagta gtggctgcga cagcatcaga
     4861 gcttgcaccg ttacagtata tcgcaccata ctcaggctgc gctataggtg aggaatggat
     4921 ggaaaacgga caggatgtgc tcatagttta cgatgatctg agtaagcatg cagcagcata
     4981 ccgtacactt tctctccttt taaagagagc gcccggacga gaggcttatc cgggagatgt
     5041 tttctatctc cattcaaggc ttctggagag ggcagcgaga ctgtcagaca agctgggtgg
     5101 aggctcactc acagcccttc ctatcattga gacacaggcg ggtgatgtat cggcatatat
     5161 cccgaccaat gttatctcaa tcacagacgg tcagatatat ctcgagacag agatgttcaa
     5221 cgcgggcttc agacctgcca taaatgcagg actttcagtt tcacgagttg gaggctcggc
     5281 acagataaag gcaatgaaaa agattgcagc acctatccga gtagagctgg ctcagtacag
     5341 agagcttgca gcgttctcac agtttggctc agagcttgat gcaagcacag cagagcagct
     5401 cgcacaggga gcacgaatca aggagatttt aaagcagccg cagtacaagc cgatgcctgt
     5461 agagaagcag gttatcataa tatacgctgc gacaaagaag tatcttatcg atatcaaaat
     5521 cgaggatatt ttaagattcg aggcagagct gtttgatttc attgatacca gatatcctga
     5581 gattcctgaa gcaatcaggg agaaaaaggt gatggacgat gatacggagc aggcacttat
     5641 aaaggcaatc gaggaatgca aaaagcagtt ccgttagacg gttggatatt agtagaaggc
     5701 aggtggtaaa atggcttcga tgcgagacat aaagcgcaga aaaagcagta ttacaagtac
     5761 tcagcagatc acaaaggcca tgaagctcgt ttctaccgtc aagctgcaaa aggcgagagc
     5821 acatgcggag gcaacagatc cgtatttcaa ctatatgtac aggacagtaa gctcgatgct
     5881 tgcaaagagc ggcaatctgg agcacccata cttaaaggcc ggtgattcgc caaggaaggc
     5941 ggtagttgtt ttgacatcaa accgcggact ggcaggcgga tacaacagca atatagtaaa
     6001 gctcatcaca cacggagact tcaaaaaaga agaactcgac atatacgctg tgggagcaaa
     6061 gggcgaggag ctgcttacgt caaagggcta tcatatagtt gagagtgcat cggagatgat
     6121 ggaggacccg acctacgagg atgctgcggc actttgcaaa agagtgttaa aggcatttac
     6181 agacggtcag gttggggaga tttatctcgc atatacccat ttcaaaaaca cagttacaca
     6241 ggaggcgaag ctcataaagc tattgccggt ggagctggat gagagtgggg ccgatgagaa
     6301 agaggactcc aatattctca tgaattttga accgaatccg gaggaggctt tggagcttat
     6361 tattccaaaa tatgtgacaa gcctgtttta cggagcactg gtggagtcat ttgccagtga
     6421 aaacggagcc agaatgcagg cgatggattc tgctacgagc aatgcagagg atatgatcag
     6481 tgatctgaca ttgaaatata acagagcgcg tcagggctca atcacgcagg agcttactga
     6541 gataattgca ggagctaatg cgatagagtg atacatgcat atccggtcat gggaaggcag
     6601 gcatgtgtgt ctgtgaatgg ccaaaaaggc gagtgtatca tatgaattaa aaaagaagga
     6661 gaaactataa atggcagata aaaatgtagg taaaatcata caggttatca gtgccgttct
     6721 ggatatcaag ttttcagagg gtaatctgcc tgagataaac gatgcagtcg aggtgccgct
     6781 gaaaaacggc ggaaagctgg tcgttgaggt tgcacagcat ctgggcgacg atacagtcag
     6841 atgtatcgcc atgggaccta ctgatggtct cgtccgcggc atggacgcta tcgcaacagg
     6901 agcacctatt tctgtgccgg tgggggagaa tacacttgga agaatgttca atgtgctcgg
     6961 agagcctatc gacgaggttg agcctccaca gacagaggag aaatgggcaa tccacagacc
     7021 tgcgcctagc tttgaggagc aggctacatc gcaggagatg cttgagacag gaataaaggt
     7081 tgtagatttg ctctgcccat accagaaggg tggaaaaatc ggactcttcg gaggtgccgg
     7141 agtaggaaag acggtgctca tccaggagct tatccacaac atcgctacac agcatggtgg
     7201 atactcagta tttaccggtg taggagagcg tacacgagag ggaaatgacc tgtactacga
     7261 gatgaaggag tcaggtgtta tcgacaagac caccatggtg ttcggacaga tgaacgagcc
     7321 acctggagca cgtatgagag ttgccctggc aggacttaca atggcagagt atttccgtga
     7381 cagaggcggc aaggatgtgc ttctctttgt ggacaatatc ttccgtttca cacaggcagg
     7441 ttcagaggta tcagcacttt taggacgtat gccttcagcc gttggttacc agccgacact
     7501 gcagacagag atgggagctc ttcaggagcg tatcacatca acaaagaacg gttcaatcac
     7561 atcggttcag gctgtatatg tgcctgcaga tgaccttaca gacccggcac cggcgactac
     7621 cttcgcacac ctcgatgcca cgacagtact tgagcgttct atcgcagagc ttggtattta
     7681 tccggctgta gacccgctgg catccacatc gcgtatcctt gatccgcgta tcgtgggaca
     7741 ggagcatttt gaggtggcac acgctgtcca ggagatactc cagaagtaca aggagctgca
     7801 ggatatcatc gcaatcctcg gtatggatga gctctcagag gaggataagc tcgtagtaag
     7861 ccgtgcacgt aaggtgcagc gtttcctgtc acagccgttt ttcgtggcag gacagtttac
     7921 aggactcgag ggcaaatatg tgcctatcgc cgagaccatc agaggcttta aggagatact
     7981 cgaaggaaag catgatgatg taccggagag ctatttctta aacgttggaa gcatagatga
     8041 agtccgtgcc aagatgcagt aatgtgaggt gatttcatgg cagataacat ttttgacata
     8101 gaaataatca caccggacag ggtgttctac aagggagaga cagatttcct cgagttcaat
     8161 acccaaaacg gagagattgg tgtatacaaa aaccatatac cgctcacgac agtgcttgca
     8221 ccgggaatcg tgactatcca taatggcgat gaggagaaaa tagccgcaat ccattcaggc
     8281 tttgcggaaa tacttggaga caaggtcaca tttctcgcag agcttgcaga gtggcctgat
     8341 gagatagaca tagaccgcgc acaggccgca aaggagcggg cagagcagcg cctttcaaat
     8401 cacacagcag agattgatgt gaagagagca gagtttgcac tcaggaaggc acttatcaga
     8461 atagatgcag cgcatcatgt ataaaagaat taatttagga gcttaaaaag tcccaagatc
     8521 cacatatata atatgctgat tttggggctt tttgtgtaca aaagtggatt aagaagataa
     8581 tacaatcata cagttggcta taattggtca gttatactgg aatttttgtt aaaaacaggt
     8641 ggtttttgta agtatttgct aaattgggaa aattaaataa ttggtatagt atcataaaaa
     8701 tatgttttta aattggtata gtataaaccg cgttttgtgg atgaatggta tttttaatat
     8761 attgacaatt gggtgtaaac ataatatatt tagcttgttc gaacgagtaa ccaatatacc
     8821 agttgttatt tagaaattca gggaggtaat caataatgat taagtttgat gaggtagagt
     8881 acagaggaaa tcttacaaag cagattgcag ctattaataa aggatatgag tatgcaaaag
     8941 agataacagc agatggatat agtaatatct tttttgtagc tgttggtgga acaatggcaa
     9001 tgatgttaca ctttgcggca attgcaaaac aaaaaacaaa gattccagtg tatgttgagc
     9061 aggcagcaga gcttgttgtt gcaggaaaca cacagttaaa taaagattcg cttgtaatta
     9121 tgggatcaaa atcaggagat actaaagaga ctgtagcagc cgcaaaatgg tgcaaagaaa
     9181 gaggaataag aattgcatca atggtcattg acaaagagtc accacttggt cagttgacag
     9241 attatcaact tccattggtt gtatttaaag gggttgagta tgaatatctt tcaatgtttg
     9301 gattattcta cggattatta aattgtaatg gagattttcc agagtatgat cagtttgtag
     9361 aagcattagc aaaacttcca gatacattgg tagatgcaca gaagaaattt gatgagccag
     9421 cggatgctat tgcaaagaaa tattttaacg cagattatat gatgtggatt ggcagtggag
     9481 agctttgggg agaggtatac ctgttttcta tgtgtattct tgaggaaatg caatggatca
     9541 gaacgaaatc tataacaagt ccggagtttt tccatggaac actggagctt gtggagaagg
     9601 atatgccggt attcttagta aagagtgcag gcgtatacag aactcttgat gaaagagcag
     9661 aaaaattcct taaggagcat acagacaatc tgatagttat tgacgtaatg gactacatga
     9721 taccaggact taagcctgag tttgcaccat tagttgcccc tattataagt acagcagtgc
     9781 ttaatggacg tctttcaaaa catatagagt cttatacagg acatgacctc gatatgcgtc
     9841 gctattatag acagtttgag tactaagata tagagatgat agaagctgta ttatttgata
     9901 tggatggcat tctaattgat tctgagagag agtatgataa ggccatgaga gaggcaatta
     9961 cgagatatgg acatatgata acggacgagt ttttgatcag agtaagagga attcctgtag
    10021 aagcatttaa aaaaagtctg aaactggagt tcggaagaga ttttcctgtt gagaaattca
    10081 gaaatgattt taagtccata gtgtggcaaa atatcagaca atatggtatg cctgtaaaga
    10141 atggtgtata ccagctaata caatatctag aggtaaataa tataagatat gcagttgcaa
    10201 caagcaatga cagggcaatg gcactagaat tattagaagc agcagacctg aggggagtat
    10261 ttgagtacat ggtatgtggt gatgaagtgg aacatggtaa gccatttccg gatatatatc
    10321 agaaagctgc ggatatgctg ggtgtgaata taaaacacac attggtgatt gaagattcat
    10381 ataatgggat cagagccggt catgctgcag gcgcaagcgt tatcatggtg cctgatttag
    10441 tgcagccaac agatgagata aggcaaatga cagattatgt agtaagtgat ctgggtaaag
    10501 taatttcatg tatcagcaat ttttgaaact ggcattgata caacatcggt caattcgacg
    10561 cagactttga cagtattcaa ttacattgtt atttgaatct ttagatatta tgctgaaatt
    10621 caacaatgga gaattggttt cgattccaag taaaacagca ttatcaatgt cgcatatatt
    10681 catagaaacg ttatagctat gatgagttgg aataatgtta tagtcgtttt tcattaaagc
    10741 atataatgac tgcgtttcat tttccttcat tttccttaac aattcaggaa acatagcctc
    10801 agaaagatga gtagtttcta tcattgccgg tttctgattt ataaaccgca atcgtttaat
    10861 aatataaatt ggtgagtagg gagataatcc aagtattttg cttatataat ctgtggcaag
    10921 agtctggtag caatatatta atttattgga acgcttgaag ccgtttactt cacaataggc
    10981 atcgagtgaa aatcccatac caatacttgt tcttagtttt gggggtgcca catatgtgcc
    11041 actcccaact atagaataaa gaatatgttc attttccaat cgctttagag cttcacggca
    11101 agtggtcctg ttaatgccaa gctcttctgc catggcacgt tcagatggaa tacggtcatg
    11161 aggattaaga ttatgcatgg atatataatt ttcgatagcc tcagcagcgt catcggcttt
    11221 tacgtgaaga taatgattca taagatactc ctaatcaaaa acaaatttaa atttatcagc
    11281 taaagaatat gataatgtat attcgacaac gcgattgtgt atatcgtaca cagtattcac
    11341 tcttttaaat gaggcggaac catcgggctg ttgaagaata tcagattgcg gtttaaatat
    11401 tttgaccaaa tctatagttt gaaccgtatg atcaacaatt atattgttat cattaaaaca
    11461 gtcatataaa gattctttgg caaaattata gctttgcgca ttaggaacat aggcaaatgg
    11521 taaaaaagta tattcgacag aataaggtat tttattacct aatctttgac gatatacgaa
    11581 aaaaagttga tcagattcag aaatattaaa tatatttgaa tatttgtacc cggcattcct
    11641 tattccagat gttataattt tggttgaagg tttgagatga tggtctttta agtattttct
    11701 tgtagaggtt acagaattgg tagcatgaag cttaggcatg gatacataag tacctttacc
    11761 ttgaatcttg tatagcagac catcgtgtac aagctgctca atagcattac ggacagtggt
    11821 tcggcttaca aagtattttt cagccagtgt acgttcagac ggcagggctg aaccgaaaga
    11881 atataaattg ttctcaatgt cacctttaag ggtgtcataa atatactttg atgaatgact
    11941 atccatataa ttctccttaa aataattgta aagatatatt ataattataa aatggataat
    12001 aaagtaaatc aataaataaa ataaatataa aagaggaggt ggacagatgg ttaaaattat
    12061 gctagcgtca cacggaaatc tggcagcagg aatgctttcg tcagcagaaa tggtttttgg
    12121 aaagcaggat aatgtcagtg ttatatgtgc atatgtagac ggagaagatg atgtatcgac
    12181 aagaataaaa ggtttcattg atagcattgc accggatgac agctggatca tttttacaga
    12241 tttatttggt ggaagtgtga ataatgagtt tatgaagtat atttctaatc cgcaaataag
    12301 gcttattgct ggaatgaatc tggcacttgt tataacagca atgtctatag tagaaatgaa
    12361 aaataatgct gctgaggttg aggaagaact tttgggtata gcagacagtg taataaattt
    12421 ttgcagtagg acaggtattg attcgttaga agatgacgaa ttttaaagaa aggggtatat
    12481 aggatgatag taaatgtcag agtagaccat cgtttactac atggacaggt tgcaatggcc
    12541 tggaatcagg tattaggaag tgattgcatt ttaatagcca atgatagtgt gccaaacgat
    12601 gaaatcagaa aagctggaat aaaacttgca aaaccagcga atacaaagct tgttataaag
    12661 aatatggatg catctgtaga agctcttgtg tcaggtgtaa cagataaata taagttattt
    12721 attgttgtag agagtattgc tgatgtatac aaactgtgca aggaaacagg aatgattgat
    12781 aagatcacgc ttggtggtgt taaatcaaga gaggggacaa ggcagatatc aaaagcagta
    12841 tttacaacac caaaagagga agaactcata aaggagttga ttactgatgg agttactgtg
    12901 gagataaggc aggttccaag tgattcagta atttatgcaa aggatgtatt gtaggaggga
    12961 ctgatatgat attaaaggct atattattgg gattagttgc catgttagga catactaatt
    13021 tcttgtttgg aacaaaccta ttagaccgac cattaataat gtgtacatta actgggctgg
    13081 ttatgggcga tttaaaaagt ggaattatta ttggggcaat gatggagctt gcatttatag
    13141 gtgcattttc tgtaggggca tcacttccac cagatatgat ttctggaggt gtgcttggag
    13201 ctgctttgac acttgcagca gggaatgatc ctgaggttgc acttacaatt ggcgtaccta
    13261 ttgcttcatt agcacttttg atgaaaaatg catgtaaaat atttattttg ccgatttttg
    13321 tacacaaggc agatgattat gctgttaaag gaaatagtaa aggtgttgca agaatgcaca
    13381 tgctaggagg attcctatat ttgaatcttc cgtatggaat atttgtattt tcagcattct
    13441 tattaggaaa tacagttatt cagagtgtgt tggatgctat tccacaattt ataaaatcag
    13501 gacttacaat tgcaactggc ttgatgccgg ctctcggatt tgcagttctt gcaagcatga
    13561 taataaataa aaagaattgg tatttcttat tattgggatt tctcatttct gcatacatgg
    13621 gaatgtcagt aacaggagtt gcactttttg caacagttat agctcttata atagtaaata
    13681 tgcagaataa tcaggttata acagctacag aaatgggagg tgatgaagat gacgacttct
    13741 aaattaatta caaagagaga tcttagaaaa gtattcttca gatcttttcc aatggagtgg
    13801 acatggaatt atgaaaaaca gcaaaatcta ggttatacgt atgctatgat tccgattatt
    13861 gagaaactgt ataaggacaa accggaggaa aaagcagcag cattacagag acacttggaa
    13921 ttttttaata caactccaca tatggtaaca ttaattcttg gaattagtac agctatggaa
    13981 gaagaaaatg cgctcgatga gaattttgac acatcatcca tcaatagtgt aaaagcagga
    14041 ttaatgggac cgcttgcagg tataggtgat tcttttatgt ggggaacact tcgaataatt
    14101 gcaactggaa taggaacatc acttgctatg aacgggaata ttctgggacc aattttattc
    14161 ttcctattgt ttaatatacc acatgtaatt ctgagataca tatgtatgat gggcggatat
    14221 aagtttggag ctggattttt atcaaaaatt gcagcgggtg gactaatgga aaagcttacg
    14281 tatgctgcat caatattggg acttatgtca attggttcta tgatagcaac tatggttacg
    14341 gtgaatattc cattaacgta tacaaacgga gatacaagtg ttgttatagc tgatgtaata
    14401 aatggaattg ttccgtgtct gttaccatta ttatttacag gatttatata ctggctggtt
    14461 accaaaaaga agatgaagac aacctgggca ttgatactta tatttattat aggtattttg
    14521 ggaagtctta ctggaatact gggggtataa gtcaatattt gttactactc aatttatacc
    14581 cactggataa ttcagtgggt atattttata gaaaggattt tgaaatatgg ctaaaactat
    14641 gtttgattat atcaactctt caacagaatt aattgaggaa aatctaaaaa aacgaatagc
    14701 attgacagaa accatggtta atgaatatat caaaggacca tatcataata ttactatagt
    14761 tgcatccgga tcttcgaata atgctgctca gtgtgcaaga ctatatatgg aaaaagtgtt
    14821 gaaatgcaaa gttacagtta tatcaccatt tgaatttatt cacgcagagc atagcgttgc
    14881 gtcagatgaa atggttatag ctgtttctca gagtggatat agtgtaaata ctatggaagc
    14941 acttaagttt ataaatgatg aactgactag aaagtcaatt gtactaacag gtgacttgaa
    15001 tagtgatata gtatcggttg cagacctggc agttgatttt ggcgttggtg aggaaacggt
    15061 aggatatgta acaaaaggtg taattgcatt gtcactttat cttatgctat ttgctttgga
    15121 agccgctaaa atgtgtaaaa taataggcca gaatgaatat gacacttggg taagagaatt
    15181 tgaaaaggta agcggtgcgc atgaaaaaat gatagttgcg acaaagaaat tttatgctga
    15241 taacattaaa gcattaacat cgcttcaaac agtatatata tgtagtgtag gtgcaaatat
    15301 gggaacggct atggagggag cacttaaagt aggtgaaact attcaggttc cgggcatagc
    15361 atatgaatta gaagaatata tacatggacc taatcttcaa cttacaccaa attatacagt
    15421 gttttttatt gacggaggtc ttgcttctga tagaacacaa gagatatttg aggctacagg
    15481 cgttataacg gataaaacct atatgcttac aaataaaaaa ggacttgaag ggaaaaatat
    15541 aataaatccc gactttgaaa taaacgagct tattacacct cttgcttttt taccattttt
    15601 tcaaatatta agttaccaaa taacagagga tactcataaa tggaaaaaac atccattaat
    15661 ggatgagatg aaacggctag caggagctaa aactttaaat tatgaaagta gtcctttaag
    15721 aagggatatg ccacagtaaa aatatagtta tggaatagcg taatgatgat attgtaaata
    15781 ccattgttat gctattttta tattgagtat aatatttgtt ttaatggcaa gatatcctat
    15841 ggaaaattcc gctaaaatat gctttaatag aagatatata gcagtaaagg agatgaacac
    15901 aatggggaga tttgtaaatc ctggaaacag tgcattcaag gtagcattag cttccgagat
    15961 atatatagac aaaacaggct tgctcgattt taccaatagt gtgcttggaa caaagcaggc
    16021 atatatatgt aatagccggc cgagaaggtt tggcaagtct attacggccg atatgcttac
    16081 agcatattac agcaagggct gtgattccag agaattattt atgaactata acattgcaca
    16141 gacagaatac tttgagaaat atctcaacaa gtatgatgtt attcatctcg atatgcagtg
    16201 gatactaatg gatgccggtg cgccggaaag aatatcagga tatataaata aaaatgttat
    16261 atcagagttg gcagatctgt acaaggatat tgatttaaga gatcagaaaa cattgtatgg
    16321 tgctctatcg gttattaaca gtatgacgaa caataaattt gtaattataa tcgatgagtg
    16381 ggatgttttg atacgtgatg aagcagcaaa ttcctcagtt caggaggaat atatcaattt
    16441 cctgcgcgga atgtttaagg gaagtgaacc aacaaagtat atagagcttg catttctcac
    16501 aggaatttta ccgataaaaa agctaaagac gcagtcggca cttaataatt ttgatgaata
    16561 taccatgtta tatgcgggac gtttgtcccc atatattggc tttactgagg atgaggtcag
    16621 ggacatatgt gatcgctatg gcagggattt tagccaggta gagcggtggt atgatggata
    16681 tatgctggga gattgccatg tatacaatcc gcgtgcagta gtaaattaca tgcttcaggg
    16741 agacttaaag agttattggt cagagaccgg gtcttatgat gtcatagttc cacttataaa
    16801 tttggatttt gatggattaa agaccgcaat aattcagatg ctgtccggtg gagaaataaa
    16861 ggtgaacacc ggcagtttta tgaatgatac ggttagcttt aaaaataaag atgatgtttt
    16921 aacatatctt gttcatttag ggtatctggg atttgatcag aaaaagagtt gtgcgtttat
    16981 tccaaatgag gaaatacggc aggatattga aaatgcttgt cggcataagc tatgataaga
    17041 agacaaagaa gcatcagtgt attatagaag agtacatgaa aaaataatca gataacattt
    17101 ggatagcagg ataacgactg caagaggaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaggagac
    17221 tcaaacaccg cagcagacag ttgacgcgca gcagatggtt ataccgcagg agagcgagcg
    17281 catcttgaca tagaaataat cacaccggac agggtgttct acaagggagc gacagatttc
    17341 ctcgagttca atacccaaaa cggagagatt ggtgtataca aaaaccatag accgcgcaca
    17401 ggccgcaaag gagcgggcag agcagcgcct ttcaaatcac acagcagaga ttgatgtgaa
    17461 gagagcagag tttgcactca ggaaggcact tatcagaata gatgtggcac atcatatgta
    17521 attataaaat cccggaatct gcagataagc ggatttccgg gatttttaat ataagaccga
    17581 ttaattattt tcttttttct ctttccaatc cggctgatag gcatagtttc cccgaaagat
    17641 atgatattct ttcggggaaa tttttagaaa attatttttt tatgtaaaaa atgaaacatg
    17701 gtcatgtcct gacacggtgg gatttgcgga actgctgtgg cgattcatga aaatttttct
    17761 ggaaggactg cgaaaaatag ctggtagagg aaaacccgat acatccggca atctcttcga
    17821 tactgtgatt ggttgtgagc agcaggtcac gcgcagacgc cagccgaact tcgttcaggt
    17881 aagcgatggg agatttcccg tacagctctg caaaacggtg tgaaaggtaa tattttgaga
    17941 gactgcagtt ttcggcaagc gaatccagag agatcatgct ctggtaattg tgctcgataa
    18001 agcgttttac cttgtggcat tcgcggctgc tgttctgcgc atccaccact tcataggata
    18061 catggtcttt ccggcagaaa tataaaatta aaatatccag ataatgctgg cagatctctg
    18121 cgtagcggtc ctgtttgtta tgcatttccc gcaggatatt ttcaaaacac tggcggataa
    18181 aaggcgagtt tgcagaagcc gggacatgaa tgtattccgg ttctttgtca gtattcagtt
    18241 ttaatccttc caccccgagc acataataat ccagtggatt ttccggatcg gaaaactctg
    18301 tgtgtgttac ctgtgcaccg atcacgacaa ggctgtttga ggagacagga agagattcat
    18361 tttcaatcag catgcgtccg gtgccgtctt taataaagaa gatttccgta aaatgatggc
    18421 tgtggggaaa cgaagtccag tcattttcaa atttggatgc tgatatggaa agcaggcgga
    18481 aatggatttc cggctgtacc tcattatcca gattaaattt gcggttactc atggaaaata
    18541 cactcctttc atgtcataat tgcaatgatt tcctagttaa aatggaacga tgttgtactt
    18601 agtatacaat gaaaaaaaca aaataacaag atttttaaaa taaagcaaga attataaaat
    18661 atagagcaat atatggatag gaaaaagaga tggcgtactc tataataaca aatgtaagga
    18721 aggaaacata agaatttata gtaaaaaata acaaaaatgg gaggttacta tgaagaaaaa
    18781 aattttatgt acaatgataa gtatggtaat ggtagcatct ttatttgcag ggtgcggtac
    18841 agattcagga aaggagaatt ctgcgtcaga taccagcaag acaaaagaag ctggaacaac
    18901 aatcagtgta gctgctattg agacagctta tggttctgaa atgtggcaga aggtggcaga
    18961 tgcatttaca gaagaaaccg gaattaaagt agaacttaca acagataaaa aattagagga
    19021 tgtaattgga ccatccatgc agggaggcga atacccggat gttattcatc ttgccacggg
    19081 acgtgaagct gcgttgacgg aacagtttat taagggacat atgattactg acattacaga
    19141 tgttttatct atgaaagttc cgggagaaga caaacttgtt tcagagaaga ttgcaggagg
    19201 atttacagac acatcattga ccaatcctta tggtgatgga aagacatatc ttgcaccaat
    19261 gttttattct ccatgtggtc tgttttataa tgcaggtctt ttagaagaaa aaggatggga
    19321 tgttccaaag acatgggatg aaatgtggga attaggagat aaagcaaaag aagagggaat
    19381 ttacttattt acatatccta caacaggtta ttttgatgca ttcttctacg cactgatgta
    19441 ttcagcaggt ggtccggaat tttttgataa agcaacaaat tatgcggaag gcatctggga
    19501 aactccggaa gcacagacat gctttgatat tgttgcaaag ttagctgagt acacaaatcc
    19561 ggtcacacct gcgcaggcaa atgatcagga cttcacacag aatcagcagt tagtacttga
    19621 caataaagca atctttatgc caaatggaac atggatcgtt ggagaaatgg cagaagcacc
    19681 tcgtgcagat ggatttaagt ggggaatgac agctcttccg gcagtaaaag atggcggaga
    19741 tgcatacagt tatacatggt ttgagcaggc atggattcct tctggtgcag agcatcagga
    19801 tgcagcaaaa ttatttatct cttatttata cagtgacaaa gcatgtgaaa tttttgcaaa
    19861 agcaggagct atccagccag tattaggcat tgcagacaaa ttaagtggtg ataacgtaat
    19921 gttctactct atttatgata atggagcaaa ggcagctatg ggtaatttcg cagcatttga
    19981 agcaattccg ggcgtggaag tgcgaacagt attctttgac ccggtaaact cattagtatc
    20041 aggcgatatg gatgagcagg catggattga cggaattatt tcggcaagtg accagatgcg
    20101 ggcgaatttg aaataagagt ttttagtttg ccttaatgct atagtggtaa aaatgagtcg
    20161 gcggacagaa tttttgtccg ccgaatttta aatagtacgg tttgtttgaa aggaacgtga
    20221 aagtatgaaa aaagccagag gacgaagcgg ttttttggcg gtatgcatag caccagcagt
    20281 aattttattt tttgtgttta tgatattgcc taccttaaat gtattccgga tgtcgttatt
    20341 tgaaaaaggt gcatattcac cgaatgaaac atttgttggc ctgaaaaatt ttaaaacatt
    20401 aatatcggat actaatttta taaggtccat gcaaaacatg attttactta ttgttgttgt
    20461 tacaatagtt acctttgcat ttgcacttgt ttttgcaggc attctgtcaa gagaacaaat
    20521 caggggacag aattttttcc gtattatttt ttatattccc aatattttat cggttgtagt
    20581 aattgcaggt attttttctg caatttataa gccggagaac ggaatgctaa atagtattat
    20641 cggactgttt cataaaatgg agaacccgat tttgtggaaa ggtgaatcgc ttgtgatcgt
    20701 cagtcttatt attgcaatga tctggcaggc gattggttac tatatggtca tgtacatggc
    20761 atctatggcg agtgtgccga aaagcctgta tgaaagtgcc ggattagatg gtgcaggcag
    20821 gctcgttcag ttttttcaga ttacaattcc gttgatctgg actaatattc gtacaacatt
    20881 aacatttttt attatttcta ctattaatat ggcatttctt tttgtaaaag ctatgacttc
    20941 aggtggccca aacggagctt ctgaagtgtc actaagctat atgtatggac agaaggatgc
    21001 cggattatat ggatacagta tggcaatagg tgttgtaata tttattttct cgtttgcatt
    21061 gtctgcactt gtaaataaag ttacagagcg tgaaccactg gagttttaag gagagaaaat
    21121 atgagagaga aaaaaaatcc gaattctgcg gaacggttgt ataaattttt tatctattta
    21181 gttttaataa tgcttgcagt cagtattatt attccggtgg catgggtgtt tatggcatcc
    21241 attaagcaaa ataaagagtt ttatggaaat ccgtggactt tgccggaggg tttttatttt
    21301 cagaacttta ttgatgcctg gaatacagca aaaatgggag attacatgtt aaactccgta
    21361 ttggttacag ctttggcact tgtaattctt ttaattgttg cgctgccggc agcttactgt
    21421 ttgtcacgtt ttaaatttaa aggaag
//
LOCUS       DPUF01000146            5023 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_26173, whole
            genome shotgun sequence.
ACCESSION   DPUF01000146 DPUF01000000
VERSION     DPUF01000146.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_26173"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(220..462)
                     /locus_tag="DHV81_05230"
     CDS             complement(220..462)
                     /locus_tag="DHV81_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93887.1"
                     /translation="MEQTEIRKVRASIHQQCGNRVKIQQDLGRNKVDIQEGVIQQAYP
                     SVFTVLIDDEFEQNPPQLLSFTYTDVITKDIRMQLC"
     gene            complement(744..1199)
                     /locus_tag="DHV81_05235"
                     /pseudo
     CDS             complement(744..1199)
                     /locus_tag="DHV81_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741455.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    1090..1144
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1295..2026
                     /locus_tag="DHV81_05240"
     CDS             1295..2026
                     /locus_tag="DHV81_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCI93888.1"
                     /translation="MRSVLIVIFVFFYLLLGLPVLGAMWIIHKFAPAWADMAQLRLVQ
                     WAFRVIIFLSGADIEFIGEENVPKDEAVLYIGNHRSFFDIIITYSRCPRLTGYISKQS
                     ITKIPVLGLWMKRLHCLFLDRDDIKQGLKVVLAAIDQVKSGISICVFPEGTRCKNKDD
                     LTDVQSFKEGTFKIATKSKCKIVPMAIMGTNEIFEDHLPWVKKRHVVIRYGTPIDPAT
                     LSKEEQKHLGAHCREVIVDMLHEMV"
     gene            complement(2114..3835)
                     /locus_tag="DHV81_05245"
                     /pseudo
     CDS             complement(2114..3835)
                     /locus_tag="DHV81_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569123.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     assembly_gap    3337..3416
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4352..>5023)
                     /locus_tag="DHV81_05250"
     CDS             complement(4352..>5023)
                     /locus_tag="DHV81_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI93889.1"
                     /translation="FTETTLLNFPRAFFNTLFISVFSCIISTVFVLSVAYCMSRLRFK
                     MRKPFMNLAMILGLFPAFMSMVAVYFILKAIGLTEGSVLRLSLILIYSAGSGVGFYIA
                     KGFFDTVPKSLTEAAILDGCTQFQVFYKVILPLSKPIVVYTVLTAFLAPWLDFVFVRV
                     IVGPNDKYWTVSLLLYNMLEKEFINGWFTRWAAAAVIVSIPISILFVIMQRFYVDGLT
                     GAVKG"
BASE COUNT         1417 a         1176 c          856 g         1439 t
ORIGIN      
        1 ttgtccttta tgacataatc cctgtcgaag ccaacctgcg acatatcctt taccttttct
       61 atgcattgtc tgattttttg tgtaattaac tccataaaaa ataccccact tcaaatatgt
      121 atataaaata catattcaag atggagtaac tttatgcaga tgcaaatatt taaattcatg
      181 atcattataa taaagatgta acatctttac ttcatgatac tagcaaagct gcatccttat
      241 atcttttgta atcacatcag tgtaggtgaa agataagagc tgtggcggat tctgctcaaa
      301 ttcatcatct atcagaaccg taaacacact tgggtatgcc tgctgaataa caccctcctg
      361 gatgtcaacc ttgttgcggc ctagatcctg ctggattttt accctgttgc cacactgctg
      421 gtgaatcgat gctctgactt ttctaatttc tgtctgttcc attttaagcc tccttattgt
      481 ggatatgccc actggcacat aactatagtc ctcatggtaa ccagtattgt aacgacggtg
      541 tcatatatat gaaatcaatc tctgtataaa tataaaacag gtcaaattaa tcaacaatta
      601 tatcaatcaa tatatttgaa tatataaata tgtcaccaca atatctagta actttactat
      661 agcaaagtat cgcagtgata gcaatagggc attagtactg attgtcagtt ttttcgcaac
      721 ttttaacaca atttacgaaa ttttcactca aacacaacag gcatttcact caattccgtc
      781 tttattacta tatacggata actcgcctga ttgctgacct tctcgccctt atccggggct
      841 atcagacgcg tatcaaaata cattgcctta tccttaacat aaaactggtt aaccgtaata
      901 ctgtagccac ctgtctcctt tgtgccaaag ccttccgcta tgtataaata ttcatcatcc
      961 ttgtacgtca gcttcatcgc ctttgccttg ttttcattta tctgcttttt aaactcctcc
     1021 gggatttcat ccccgcagat caccgtgtac tcaaccatcc tgtcactctc cgtgcctgaa
     1081 atatttgcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnatataa caagcacacc tgcagccacc accagaattt ttctcaattt ttctgacatt
     1201 ttttcctctc tttcttatca tatcgcttat gatatgatat aatatcaaat attgaactac
     1261 aatttatata gagtatgaaa aagagaggtc aaatatgcgt tcagttttaa tcgtaatatt
     1321 cgtatttttc tatctgctgc tcggtcttcc ggtgctcgga gccatgtgga taatacacaa
     1381 atttgccccg gcatgggccg atatggcaca gcttcgcctg gtacagtggg ctttccgtgt
     1441 catcatattc ctaagcggag cagacattga atttataggg gaggaaaatg tgccaaagga
     1501 cgaggctgta ctatatatag gaaatcaccg tagtttcttt gacatcataa tcacctactc
     1561 cagatgtcca cgcctgaccg gctacatctc aaagcagagc atcacaaaaa taccggtact
     1621 gggtctctgg atgaagcgcc tgcactgcct gtttttggac cgcgatgaca tcaagcaggg
     1681 actaaaggtt gtgcttgcag ccatagatca ggtcaaatcc ggcatcagca tatgtgtatt
     1741 tcctgagggc acacgctgca aaaacaaaga tgacctgaca gatgtacagt ctttcaaaga
     1801 aggtactttc aagattgcca caaagagcaa atgcaagata gtgccaatgg caatcatggg
     1861 aacaaatgaa atattcgagg atcaccttcc atgggtcaaa aagaggcatg tggttatccg
     1921 ctatggcact cctattgatc cggcaacact cagcaaagag gaacaaaagc atctcggagc
     1981 tcattgcaga gaagtgattg tggatatgct tcatgaaatg gtatagctga aaaaccgccc
     2041 agtcgggcgg tttttcatat ttcttcttca aattccttta aactattaaa tttttattct
     2101 catcaactat tttttacttc aaaacaagca ccgaatacgc cggcagcttt gccgttccat
     2161 ccttatatga cggtgctttc tttgtagtct gaagcattgc aacagcatca tttactccaa
     2221 gcgatgacac atccacactt gcttcatcct ttgatggatt aaatataagc actgctttct
     2281 caccattgta ctctcttgtg aatatacaca cattatcatt tgacagatcc ttttcaaagg
     2341 tatttgtacc tcgtgctatc tccgggaaag catttcttat tgatattgtc tgcttcacaa
     2401 agttgtaaat agagtatgga tcctccatct gcttatccag ggtatcatat gtctgctcgg
     2461 tttcttctgc attctgaggt cccacacaca ttcccttagc ttttgaatcc ccgctccatc
     2521 tcatggcaag acgcttcgtc tcatcattgg ctgtgcctct catgccgatt tcctctccat
     2581 aatacaaaaa agcatttccg ggcataagaa gattcatagc ctgcgccatc ttggttttct
     2641 cctcggcatt atctccgttg tagtatcctg cgctgcgtcc catatcatga tttgtataaa
     2701 aaggtgcatc aatgtatgag tctgtatact tctttatctc attttccaca tcaaccaaag
     2761 cattgccgta tgtgctggca ccatgatttg ctgcaccatt taacaccttt ccaatatatc
     2821 catcctgctg tgaaaaatca aagttgaaca tactgtcaat tccactctta tagtacttcg
     2881 catactctct gtatgtagtc cagccctcac caaccatata agcatcggcc tttttgctct
     2941 tcacattgtt cacaagccat gtgaggtcat ctatagtctc agtctggtta ttattattgt
     3001 aataaatcac agcatccagt ctaaagccat ctacgccgcg gtcaagccag aaggatgtaa
     3061 ccttgtcaat ctcccctctt acagcttcgc tctgcaggtt aaggtcaggc atgctgtcta
     3121 caaactgtga ctcgtaatac caattcgtgc ccggcagctg gttgtagcct ccggtattgg
     3181 ttttgctaaa attatagtaa tcaacataag gacattccga agaatccggc tctgccccat
     3241 caggaagcga cttgatataa tcagcagccg ccttaaacca cggatgctcg ttagatgtgt
     3301 gatttattac aaagtcaata atcacattga tgtttcnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttgt
     3421 caatattcat ataatccgta atatcatact tgtggtatga aggagaaggc ataataggca
     3481 tcatccagat ttcattgcag ccaagtccct cgatatagtc aagcttctct gtaagtccct
     3541 ttaaatcccc aatgccgtca ccgtcactgt caaaaaagct gtatacgaac acctcatagc
     3601 aggttctgta attgtcctga atcacattag gatcattttt cgcattcatc tgctccatag
     3661 cagaaggctt gtactccttc tttgctgccg tctcggtatt ctttccaccc gcagcacatc
     3721 cagcaagcat cgacaccgcc atagctgctg tcaataccac agcagtcaac ctgcccgcca
     3781 atctattcac agccctgccg accatgttgt ggccgcctat tttattatta atcatacgca
     3841 ctccttttct tattttctaa actaaattct gagcaaactt gtatctacaa aatcactaca
     3901 catattcgct aaaacatacg attctcttgc tttgtccaca ctccatttca atacgcactg
     3961 aacaatcgtc caccggatat tttgcgacgc tgcaacgcag ccagcattac tcataagtat
     4021 gtagttaact caattctcac atctttcaca aggtagcaac aaacttagca cacggtaccc
     4081 tccggccgga gggtacacag tgtgttccga agaaaaatat gaactgccac ggggtaccat
     4141 gccccacaaa caagccacac acctcatctc tccaaatcca agcagcgaag aaagcccgtg
     4201 gctgacttca tattttcctg aggaatatac tgtgtgccat gccggcacca cacaacctga
     4261 aagtgctaag tttgccacac accccaaaat ttgccatcta tataaaccaa aagccgccgc
     4321 acaaaagtgc agcggcctaa ataaaaccaa attagccttt gacagctcct gtaagtccat
     4381 ccacgtagaa tctctgcatg ataacgaaaa gtattgagat aggtattgat acaataactg
     4441 cagcagcagc ccatcttgtg aaccatccgt ttataaactc tttctcaagc atgttgtaaa
     4501 ggagaagaga tactgtccaa tacttgtcgt ttggaccaac gataaccctt acaaatacga
     4561 aatcaagcca tggagcaagg aatgctgtaa gtactgtata tactacgatt ggcttactga
     4621 gtggcaggat aaccttgtaa aatacctgga actgtgtaca tccgtcaagg atagccgcct
     4681 cagtaagaga cttaggcaca gtatcaaaga atccctttgc gatatagaag cctactcccg
     4741 atcctgctga gtatatgagg atcagtgaaa gacgaagtac gctaccctct gtaagtccga
     4801 tagcctttaa gataaagtat acggcaacca ttgacatgaa agccgggaac aatccaagaa
     4861 tcatagccag attcatgaaa ggcttacgca tcttaaatct cagtcgtgac atacaatacg
     4921 caacagaaag tacaaatact gttgaaatga tgcatgagaa cactgatata aacagtgtat
     4981 taaaaaatgc tctcgggaag ttcaacagtg tagtctctgt gaa
//
LOCUS       DPUF01000147            6771 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_19376, whole
            genome shotgun sequence.
ACCESSION   DPUF01000147 DPUF01000000
VERSION     DPUF01000147.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_19376"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="DHV81_05255"
     CDS             complement(<1..369)
                     /locus_tag="DHV81_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HCI93890.1"
                     /translation="MVWKINNNCQIFIEKTDSIAGRQKEIIPHVSAYYVDKYTTISEK
                     GVKTGINRLCGTANQELLAGYMLYKYLGISSDEQLVRNEHGKPEIAAGYKNESAAHSG
                     PYVVLAISDRPVGVDIEQTKR"
     gene            complement(473..2233)
                     /locus_tag="DHV81_05260"
     CDS             complement(473..2233)
                     /locus_tag="DHV81_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93891.1"
                     /translation="MNSSKNISKKEMNKKSSTMRLIAYMKPYAHWVIFALLLVLGLTA
                     FDLYRPMLVGDAIDTFGANGDYDVIIATAIKYAVVLALSFAFNIAQTWILQKTGQNII
                     LQMRKDLYRHIQSLGSRYFDITPVGKLVTRVTNDVEALNEMYSGILVQLFRNIVKIVG
                     LAGVMLVLDVRLAAISFVLMPLVIGLTVLCQKIARNIYRLYRTRLTDINTFLSEHLSG
                     MKIIQIFGRQERKFEEFHDKNTKLYKAFYREMLMYAVFRPLIYILSILSLMIVLWFGS
                     RNVFDEIISVGTLYIFSNYIRSFFDPIQELAEQFSTLQSSIASAEKIFTVMDEDEFIP
                     EVENPKQPDKITGKIEFDHVWFAYDGENYVLKDVSFVINPGEKVAFVGATGAGKSSIL
                     NLIGRYYDIQKGHIYIDGIDIRQLSKKQLRSAIGQMQQDVFIFEGDVAYNIRLNDNDI
                     TDEQVKEAAEYVNASHFIEKLPQGYHEPVTERGATFSAGERQLLSFARTLAHNPSILV
                     MDEATANIDTETEILIQEALEKLMDGRTTIMVAHRLSTIQHADCIMVMHKGRICERGT
                     HRELLEQDGIYRKLYELQIS"
     gene            complement(2303..4163)
                     /locus_tag="DHV81_05265"
                     /pseudo
     CDS             complement(2303..4163)
                     /locus_tag="DHV81_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568351.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     assembly_gap    3893..3993
                     /estimated_length=101
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4478..5140)
                     /locus_tag="DHV81_05270"
     CDS             complement(4478..5140)
                     /locus_tag="DHV81_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCI93892.1"
                     /translation="MKIKSLFESKFIKVFDLQYQEGRHYYNATRRDEEDLVAAKSTEE
                     FKKMLPDAVSCVVIWNPSGDDEKSGHEPCLLMNREFRYPTGQYLLSVPAGLIDPEDCT
                     GDNDNTAPLIKTAMRELHEETGLKVIEKDTVSVINPCLFSTPGMTDESNALVKIVLNR
                     DSLNGMSQEGAVGGELFDGFDLLTKAQAKKILEDGVDEHGIYYSVYTWAALTYFVADL
                     WR"
     gene            complement(5160..5900)
                     /locus_tag="DHV81_05275"
     CDS             complement(5160..5900)
                     /locus_tag="DHV81_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCI93893.1"
                     /translation="MAELENLFALQDKNYREFHARLMPNIEKERIIGIRVPVLRKYAK
                     ELAHGSRLFLSVQKEKRVCNTVKENDCDNVEKFLNTLPHYYYEENNLHMFLIMQMRDY
                     DTALAYLEAFLPYIDNWATCDSGVPAVFKKHKNELLLHVYEWLDSDKPYTVRYGIGTL
                     MRLYLDEDFDIKYALDVAKIRSKEYYVNMMKAWYIATALAKQYDAVLPILQERLMDSW
                     SHNKAIQKARESYRITPQQKEYLSTLKV"
     gene            complement(5888..>6771)
                     /locus_tag="DHV81_05280"
     CDS             complement(5888..>6771)
                     /locus_tag="DHV81_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCI93894.1"
                     /translation="GKLENHYAIIGMAHVAGDYPLYYDAINEKGLGMAGLNFVGNAAY
                     AAADENSSCENGTCGIAKTKVAQFEFIPWILSLCATVADAKEKLNRILLVDTPFSSQL
                     PVAQLHWIIADKNECIVVESMADGMHVYDNPVGVLTNNPPFPYQMAALNNYRGLSTKQ
                     PENTFAPGVELSAYSRGMGGLGLPGDLSSQSRFVRVAFTKQNSKSDDSENASVSQFFH
                     ILGSVDQQRGLCEVTEGKYEITLYTSCCNCDKGIYYYTTYDNSQITAVDMNRENLDEK
                     LLIRYPVITEGKICWQN"
BASE COUNT         1681 a         1725 c         1293 g         1971 t
ORIGIN      
        1 gcgttttgtc tgctctatgt ccacgccaac gggcctgtcc gatattgcaa gcaccacata
       61 cgggccgctg tgtgccgcgc tctcattctt atatcctgct gctatttccg gcttgccatg
      121 ctcatttctg acaagctgtt catccgatga tatccccaga tatttgtaga gcatgtatcc
      181 cgccaaaagc tcctgatttg ctgtgccaca cagcctgttg atgcctgttt ttacaccttt
      241 ttctgaaata gttgtgtatt tatccacata ataggcagac acatgtggaa ttatctcctt
      301 ctgccggcct gcgatggaat ccgttttttc tataaaaatc tgacagttat tgtttatttt
      361 ccataccatc ccaagtcacc tttctataac tccaaccttc ccctgccttc atcaaaatac
      421 aactagtatt ataaattacc catatgatat ctgcagctta tactaattat ccctacgata
      481 tctgcagctc atacagcttc ctgtatatgc catcctgttc cagaagctcc ctgtgcgtac
      541 cgcgctcaca tatacgtccc ttgtgcatga ccatgatgca gtcagcatgc tggatagtgg
      601 acagacggtg cgcaaccatg attgttgtcc tgccgtccat gagcttttca agcgcctcct
      661 gtatgagaat ttctgtctct gtatctatgt tggcggttgc ctcgtccatg accagtatgc
      721 tcggattatg ggcaagtgtc cttgcgaagg acaatagctg acgctctcct gccgagaagg
      781 tcgcaccacg ctccgtgacc ggctcgtgat agccctgtgg aagcttttcg ataaagtgtg
      841 aggcgtttac atattctgct gcttctttga cctgctcatc cgtaatgtca ttgtcattta
      901 atcgaatatt gtacgccaca tccccctcga atataaacac atcctgctgc atctgaccga
      961 ttgcactgcg aagctgcttt ttgctaagct gtctgatatc gatgccatca atgtagatat
     1021 gtcccttttg tatatcataa tacctgccta taagatttaa aatagatgat tttccggctc
     1081 cggttgcacc gacaaaggcc accttctcgc ccggatttat gacaaatgag acatccttaa
     1141 gcacatagtt ttcgccgtca taggcaaacc agacatggtc aaattcaatt tttccggtga
     1201 ttttatccgg ctgcttagga ttttcgacct caggaataaa ttcatcctca tccataaccg
     1261 taaatatctt ctctgctgat gcaattgatg actgcagtgt cgaaaactgc tctgcaagtt
     1321 cctgtatcgg gtcgaaaaat gacctgatgt aattcgagaa aatatacaga gtgccgaccg
     1381 aaataatctc atcaaacaca tttctgcttc cgaaccacag cactatcatc agcgacaaaa
     1441 tgctcagaat atagataagc ggacggaata ccgcatacat gagcatctcc ctgtaaaaag
     1501 ccttgtagag ctttgtattt ttatcatgaa attcctcaaa cttgcgctcc tgcctgccaa
     1561 atatctgtat aattttcatc cccgaaagat gctcagacaa aaatgtattg atgtccgtca
     1621 ggcgcgtacg gtatagcctg taaatatttc tggcaatttt ctggcacagc actgtaagac
     1681 caattacaag cggcatcagc acaaatgata tcgcagcaag cctcacatca agcacgagca
     1741 taacgcctgc aagtcccact atttttacaa tatttctgaa aagctgtacg agaatgcccg
     1801 aatacatctc attgagagcc tcaacatcat ttgtcacacg ggttacaagc tttccaaccg
     1861 gcgtgatatc aaaatacctg ctgccaagag actggatatg tctgtagagg tcttttctca
     1921 tctgaagaat gatattctgg cctgttttct gcagtatcca ggtctgagcg atgttgaatg
     1981 caaagcttaa ggcaagtaca accgcatatt tgatagctgt ggcaattatt acatcataat
     2041 ccccatttgc tccaaaagta tcaattgcat ctcccaccag catcggcctg taaaggtcaa
     2101 aggctgtcag tcccagcacc agcagaaggg caaaaatcac ccagtgggca tacggcttca
     2161 tgtaggctat gaggcgcatt gtggagcttt ttttattcat ttctttttta cttatatttt
     2221 tactcgaatt catactataa tcttctctgt tatttcgtaa ctattcatcg tcaaatctgc
     2281 atagcaacgc ttttcaaaca ctttaactgc caagctcact ctcaagctgc tgcttatggc
     2341 tgatatcagc gtaaaaaccg ccaagctcaa gcaactcttc aggagtacca tactctgtca
     2401 gttttccgtc agaaagcact gccacatggt cagccttctg cagtgtggaa atcctatggg
     2461 caatcactat ggttgtcttg ccctgtctgg tttccatgag gttttcaagt atctgctcct
     2521 cagtatcggt atcgacagca gaaagcgaat catccagtat gaggattgca gaatccttta
     2581 aaagtgctct ggctatcgag cttcgctgct tctggcctcc cgaaagtgtc actccgcgct
     2641 ctccaaccat ggtcttgtac tcatccggaa agcccatgat attatcatgt atacacgcgt
     2701 cctttgcggc ctgttttacc gcatcaagaa ttgccgggtt gtcggcaatc ctgtcctcaa
     2761 gaccaaatgc tatattctcc tcgagtgtgt cggaaaacag gaaattctcc tgtggcacat
     2821 atgagatatc acggtgtaaa accgccagcg gatagtctgt tatcgggcgg ccatccaaaa
     2881 gtatcatgcc gttttcacaa tcatagactc taagcaaaag gtcggcaagt gatgattttc
     2941 cgcttccggt acgtcctaag ataccaagca tctcgccctg cttcacatgt atactcacgt
     3001 ctgaaagcac cggcttggag ccgtcagggt atgtgaagct gaggtggcta agctgtatat
     3061 cgccatgtat ttgtatttta tcataaatac catcagctaa attatctgta tttctgcctg
     3121 ccatgccatc attttctatt acattttccg gtggcaccat atcgacaata tcaggctttt
     3181 cctcgaaaat agtctggatt ctgccccatg ccgcagccgc ctgagaaaat gtgttgatac
     3241 agtcgcctgc cgcaatcata ggccacacaa gcatatcgat gtatgagttg aatgccacga
     3301 actggcctat cgaaacctgt ccgtcaagca ccatcctgcc acctgcaagg agtgtcacta
     3361 tcacgcttat tccgattacg gcatccagcg tcgggccaaa gacagcgcga agctttacca
     3421 cagcaagatt tttctccctg ctgttctcgc aagccttttc aaactgctta aagtcctctt
     3481 cttcctgcgc aaatgccttg accacgcgga taccggcaat gctctcctgc accttgtcag
     3541 acagatagga gaaagcatcc tgccttttca cctgacgttt ctttgactct atcccataaa
     3601 aatagcagcc gattgcaatg aaaataagtg gaataaaagc aaataatgtc agcttaaaat
     3661 ccacgtatac taccattttg acaagcacca tcaccgtcat gattaccgcg tcaaacacgg
     3721 tgatgacagc cattccggtt cccatcctga cagcctgtaa atcgttagtg aaatatgcca
     3781 taagatctcc tgtcttgtgg ctgttataat agcgcgctga gagtgtctcc atatgtgaaa
     3841 acatatcatc gcgcagtctg tattctattc ctcttgatgc tcctatgatg aannnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacgcccg ccatatcaag ctttcctgca
     4021 gtgattccgt cgattatctc cccaataaac tgcggaatgt acagatttgc aaggtctact
     4081 gcaagaagca caaaaatgcc tatcgcatag tgccatttgt atgcaagcag atatgggcgc
     4141 agcgagtatt ttttatcgtt catgttattc ctccgtattt taaatgccat tgtatggcaa
     4201 gcatgcgacc gcatggttag cccggcatgc ggtcatatgc tctctaggag ctaacgctac
     4261 gctgttaaga ggctgtacga attatgctca tagtttttct atgctcgacg aaaccggctt
     4321 ttatgtgccg tccatggcac ataacgcctt gccccggcgt ccatgccggt gcatttctgg
     4381 ttaccagggc ataattctac agactctaaa cagcttcgct agtcgctcta cgagagcata
     4441 tggccgcctt cctggctaac cacgctacat tctctatcta cctccacaag tctgctacga
     4501 aatacgtgag tgccgcccat gtatacacag aatagtatat gccatgctcg tccactccat
     4561 cctcaagtat cttcttcgcc tgcgccttag tcagaaggtc gaatccgtca aatagctcgc
     4621 cgcccactgc tccttcctgc gacattccat taaggctatc gcggttaagt acaatcttta
     4681 caagagcgtt gctttcatct gtcatgcccg gtgtactgaa aagacatgga ttgattacag
     4741 aaaccgtgtc tttttctatc actttaaggc cggtctcctc atgaagctca cgcatggcag
     4801 tttttataag tggcgctgta ttatcattat ccccggtaca atcctccgga tcaataagtc
     4861 ctgcaggtac gcttagcagg tactggcctg tcggatatcg gaactcgcga ttcattaaca
     4921 gacatggctc atgacctgat ttctcgtcat cacctgacgg attccatatc accacgcagc
     4981 tcaccgcatc cggaagcatt ttcttgaact cctctgtgga tttggcggct acaaggtctt
     5041 cttcatctct ccttgtggca ttataataat gcctgccctc ttggtactga aggtcaaaca
     5101 cctttataaa tttcgattca aataagcttt ttattttcat aaatttttgt ctcttttcat
     5161 taaaccttta atgtgctcag atactccttc tgctgtggcg tgatgcgata gctctcacgc
     5221 gccttttgta tcgccttgtt gtgactccag ctatccataa gcctttcctg cagtatcggc
     5281 agcactgcat catactgctt tgcaagtgcc gtagcgatat accacgcctt catcatgttt
     5341 acataatact ccttggaacg gatttttgcc acatctaaag catattttat atcaaaatcc
     5401 tcatcgagat ataggcgcat caaagtgcca atgccatacc tgacggtgta gggcttatcc
     5461 gaatccagcc actcatatac atgcaaaaga agctcatttt tgtgcttttt gaacacagcc
     5521 ggcacaccac tgtcgcaggt cgcccagttg tctatgtatg gcagaaatgc ttccagataa
     5581 gccaatgctg tatcataatc cctcatctgc ataatcagaa acatatgcag gttattttcc
     5641 tcatagtagt aatgtggcag agtgtttaga aacttttcca cattgtcaca gtcattttcc
     5701 ttcactgtat tacagacccg tttttctttt tgaaccgaca aaaagagcct gctgccatga
     5761 gcaagctcct ttgcatactt tctaagcacc gggactctta ttcctattat gcgttctttt
     5821 tcaatattgg gcataagcct cgcatgaaat tccctgtaat ttttatcctg cagcgcaaac
     5881 agattctcta attctgccaa caaatctttc cctccgtaat aacaggatat ctgataagca
     5941 gcttctcatc aagattctcc ctgttcatgt ctaccgctgt gatctggcta ttgtcatatg
     6001 tggtgtaata atagattccc ttatcgcagt tgcagcatga ggtatagagt gtaatctcat
     6061 atttgccctc tgtcacctcg cacagacctc tctgctggtc tacagagcct aatatgtgga
     6121 agaactggct cacacttgca ttctcactgt catccgattt ggagttttgc tttgtgaatg
     6181 ccacacgtac aaaacgtgac tgcgatgata aatctcccgg cagtcccagg cctcccattc
     6241 cgcggctgta tgcactaagc tccactcccg gtgcaaatgt attttcaggc tgctttgtgg
     6301 ataagcctct gtaattattg agtgcagcca tctgatatgg gaatggcgga ttgtttgtga
     6361 gcacacctac cggattgtcg taaacatgca taccgtctgc catcgactcc accacaatgc
     6421 actcattttt gtcggctata atccagtgaa gctgtgcaac cggaagctga ctgctaaatg
     6481 gtgtatctac cagtaaaata cgatttaatt tttcctttgc atccgctacc gtagcgcata
     6541 gtgacagtat ccacggtata aactcaaact gtgccacctt tgtctttgca atgccgcatg
     6601 ttccattctc gcatgagctg ttttcgtctg ctgccgcata cgctgcattt cccacgaaat
     6661 tcagtcctgc cataccgagt cccttttcat ttatagcatc atagtaaaga ggatagtcac
     6721 ctgccacatg tgccattcca atgattgcat aatggttttc aagctttccc g
//
LOCUS       DPUF01000148            3253 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7153, whole
            genome shotgun sequence.
ACCESSION   DPUF01000148 DPUF01000000
VERSION     DPUF01000148.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7153"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="DHV81_05285"
     CDS             <1..438
                     /locus_tag="DHV81_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCI93895.1"
                     /translation="GVYRVKPGARVCELVDMAGGMLDTADKSFWNLASELYDGQMLYF
                     PTVEELKSDHIPEGTYTTGIDGSLMKGGAEEHIGVKDGKLNINTATVEQLMALSGIGE
                     SKAKSIVEDRAKNGLFSSIEDITRVSGIGEAMFNKIKDDITVN"
     gene            449..1141
                     /locus_tag="DHV81_05290"
     CDS             449..1141
                     /locus_tag="DHV81_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93896.1"
                     /translation="MAKKVLVVDDEKLIVKGIRFSLLQDGMEVDCAYDGEEALNRAKA
                     NSYDMILLDVMLPKMDGFEVCQAIREFSDVPIVMLTAKGDDMDKILGLEYGADDYITK
                     PFNILEVKARIKAIMRRTSPERAPQVQSSVIEKGDIKLDCDSRRLFINDNEINLTARE
                     FELLEILIKNENKVYSRESLLKIVWGEDYPGDVRTVDVHVRRLREKIEANPSEPKYVH
                     TKWGVGYYYNQK"
     gene            1163..2614
                     /locus_tag="DHV81_05295"
     CDS             1163..2614
                     /locus_tag="DHV81_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI93897.1"
                     /translation="MFKSKVHFAFYKSLKSRLIFMIFVLVLVPEVVLCFGILNTYESR
                     AVSIRESEVLSQAKILANKISTSKYFDKDGSDLSNIDAQIDTLTTIYEGRVLIVDKHF
                     KIIKDTYNLDDGNTIVSEEVIKTFSASEITNYDSKNHYIELSVPISNPNDKDSKIEGA
                     LVVSVSTDNISLNMSYLTQIAVTVAIIAGAVVLFFGVLVLLHLFVPIKKLSDEISDIK
                     DGFGKEQLDVDDYAETAIISRNFNELMSGMKVMDESRQEFVSNVSHELKTPLTSMKVL
                     ADSINSMGDEAPIEMYREFMGDITSEIDRETKIINDLLSLVKMDKSAATLNIESVNIN
                     DLLEQIMKRLQPIADKQQVELVLESFRPVTAEIDEVKLTLAFTNLIENAIKYNDNETG
                     GYVHVSLNADHQYFYLKVEDNGIGIPEESLEHIYERFYRVDKSHSREIGGTGLGLAIT
                     RSSILMHRGAIKAHSVMGEGTTFDVRIPLNYIV"
     gene            2625..>3253
                     /locus_tag="DHV81_05300"
     CDS             2625..>3253
                     /locus_tag="DHV81_05300"
                     /inference="COORDINATES: protein motif:HMM:PF10646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation and spore germination"
                     /protein_id="HCI93898.1"
                     /translation="MKRIIIFVLTAVILLSMGGCTKKKADNTVEVFCLNMDVTSSVPE
                     SYVLRSKNSDVDKQVDELLGRLQDKPEDSRLRKAIPDEVSVRGHDEKNYNCTVDFSKE
                     YYDLTAAQEVLLRASVVRTLTQLDSISYVTFTVNSIPLVDSDDEIVGSMGADDFVENP
                     GEQINTSVKETLTLYFADKDGTGLEKQTRVIHYSSNIALEKLVVEQLIEG"
BASE COUNT          961 a          567 c          868 g          857 t
ORIGIN      
        1 ggtgtataca gagtaaagcc gggcgcgaga gtttgtgagc tcgttgatat ggcaggaggc
       61 atgctggata cagctgacaa aagcttttgg aatctggcat cagagctgta tgatgggcag
      121 atgctgtatt tcccgactgt agaggagctt aagtcggatc acatacctga gggcacatat
      181 accaccggca tagatggctc gctgatgaag ggcggcgctg aggagcatat aggcgtaaag
      241 gatggcaagc tcaatatcaa taccgcaact gtcgagcagc taatggctct ttccggaatt
      301 ggtgagagca aggcaaagag tattgttgaa gatcgcgcca aaaatggatt gttttcttcg
      361 attgaggata tcacgagggt gtccggcatc ggtgaagcga tgtttaataa aataaaagat
      421 gatataacag taaattagga gtgtataaat ggcaaaaaaa gtgctggtcg ttgacgacga
      481 aaagttaatt gtaaaaggaa tacgtttcag tctgctgcag gatggcatgg aggtagactg
      541 cgcatatgac ggtgaggagg ccttaaatag ggcgaaggcc aattcatatg atatgattct
      601 tttagatgtc atgcttccaa agatggatgg ctttgaggtg tgtcaggcaa taagggagtt
      661 ttctgatgtc ccgattgtca tgctcactgc aaagggcgat gacatggaca aaattttagg
      721 gcttgagtac ggcgctgatg actatatcac aaagccattt aatattcttg aggtaaaggc
      781 cagaatcaag gcgatcatgc ggcgtacttc accggaacgc gcaccacagg tacagtcatc
      841 tgtaattgaa aagggtgata tcaagctcga ctgtgacagc agacgtctgt tcataaacga
      901 taatgaaatc aatttgactg caagagagtt tgaactgctc gagattctga tcaaaaatga
      961 aaataaagtg tacagtaggg agagcctttt aaagattgta tggggcgagg attacccggg
     1021 tgacgtgcgt acggttgatg tgcatgtcag acgtcttcgt gaaaagattg aggcaaatcc
     1081 aagcgagccg aagtacgttc ataccaagtg gggtgtggga tattactaca accagaagta
     1141 atactttcag aaaggttcta aaatgtttaa gagtaaagta cattttgcat tttataagag
     1201 tctgaaatcc aggctgattt tcatgatttt tgttctcgtt ctggtaccgg aggtggtgct
     1261 gtgcttcggc atacttaaca cctacgagtc cagagcggtt tcaatccgcg aaagtgaagt
     1321 gcttagtcag gcaaagattc tcgcaaacaa gatatccaca agtaaatact ttgataagga
     1381 tggcagtgac ttgagcaata ttgatgctca gattgacact cttactacta tatatgaggg
     1441 ccgtgtgctc atagttgaca agcattttaa gattattaag gatacgtaca atttagatga
     1501 tggcaatact attgtctcag aggaggttat caagaccttc tctgcttcag agattacaaa
     1561 ctacgattct aaaaaccact atatcgagct ttctgttccg ataagcaatc caaacgacaa
     1621 ggattcaaag atagagggag cacttgttgt aagtgtgtcg acggacaata tatcgctaaa
     1681 tatgtcgtat ctgacacaga ttgcggtgac ggttgccatt atcgcaggtg cggtggtgct
     1741 cttttttggt gtgcttgtgc ttttgcacct ttttgttccg attaagaagc tttctgacga
     1801 gatatcagac attaaggatg gctttggaaa agagcagctg gatgtagatg actatgcgga
     1861 gactgctata ataagcagga attttaatga gctgatgagc ggaatgaagg tcatggatga
     1921 gtccagacag gagtttgtat cgaatgtgtc acatgagctc aagacacctc ttacttccat
     1981 gaaggtactc gcagattcca tcaacagcat gggtgatgag gctccgattg agatgtacag
     2041 ggagttcatg ggagatatca caagcgagat agacagagag acaaagataa taaatgacct
     2101 gttatcactg gtcaagatgg ataagtcggc tgctacgctg aatatcgaga gtgttaatat
     2161 caatgacctg ctcgagcaga ttatgaagag acttcagccg attgctgaca agcagcaggt
     2221 ggagcttgta ctcgagagct tcagaccggt aactgcagag atagatgagg tgaagctgac
     2281 gcttgctttt acaaacctca ttgagaatgc aatcaagtac aatgacaacg agaccggtgg
     2341 ctatgttcat gtgtccttaa atgcggacca tcagtatttc tatctgaagg tggaggacaa
     2401 cggtataggt ataccggagg agtccttaga gcacatctat gagagatttt atcgcgtgga
     2461 caagtcgcat tcgagagaga ttggcggtac aggtctggga cttgccatta caagaagctc
     2521 tattcttatg cataggggag ctataaaggc tcacagtgtg atgggagagg gcactacctt
     2581 tgatgtgcgt ataccgctca actatattgt ataggaggct tgacgtgaaa agaataatta
     2641 tattcgtgct cactgcagta attctgctct ccatgggcgg ctgcacaaag aaaaaagcag
     2701 ataatacggt tgaggtgttt tgtctgaata tggatgttac gagcagcgtg ccggagtcat
     2761 atgtgcttag aagcaaaaac tcagatgtag acaagcaggt ggatgagctt ctaggaagac
     2821 ttcaggataa gcctgaagat tcaagactgc gcaaggcgat tccggatgag gtgtctgtga
     2881 gaggtcatga cgagaaaaac tataactgca ctgtggattt cagcaaggag tattatgacc
     2941 ttacggctgc acaggaagtg cttttgagag cttctgtagt ccgcacgctc acacagctgg
     3001 atagtatttc atatgtgaca tttaccgtaa acagtattcc tcttgtggac tcggatgatg
     3061 agattgtcgg tagcatgggt gctgatgatt ttgttgagaa tccgggtgag cagattaata
     3121 caagtgtgaa ggagacactc acgctttatt ttgctgacaa ggatggtaca gggcttgaaa
     3181 aacagaccag agtgatacat tacagctcaa atatagcgct tgaaaagctt gttgttgagc
     3241 agcttattga ggg
//
LOCUS       DPUF01000149            7652 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6384, whole
            genome shotgun sequence.
ACCESSION   DPUF01000149 DPUF01000000
VERSION     DPUF01000149.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6384"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1321)
                     /locus_tag="DHV81_05305"
     CDS             complement(<1..>1321)
                     /locus_tag="DHV81_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoprotein phosphatase"
                     /protein_id="HCI93899.1"
                     /translation="LCRACEYLRALEHNENPVIAGLLPDREEAFATAVSGLSGIISTA
                     NVMAVKCTADKIPDESEKIQLEVTGRLCACCEGCSVCWTSGASISDSIKMLADAVRKR
                     MKTEEIVQNRYVDGCPHYTRMVEAATEAFARIELNEAWYRRLTENRRVIAAQLDAMAE
                     LMDSWCRAEKCIDKKRRLRLSRVYVYTKEAGIQVENAHIYENARQQVCIKADVCTKID
                     GGIEISKYVQAVSRAMGVKLRQAHGTVSIISDERTSIVLYEENQFYALSGVATKKKTG
                     SQANGDSCSMFQLDDGMYHVCVSDGMGSGKQAQAESTLVVDLLEKLLEAGFSRESALK
                     LMNSAMVISAGEESYSTVDFATIDMYTGELELAKTGAAPSFIKSGKKVSVIENESLPA
                     GVDAAQESKHSKNTLQSGDFLVMVTDGVLEYLHVKDRQGKLMDIIAGV"
     assembly_gap    1322..1457
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1458..1750)
                     /locus_tag="DHV81_05310"
     CDS             complement(<1458..1750)
                     /locus_tag="DHV81_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoprotein phosphatase"
                     /protein_id="HCI93900.1"
                     /translation="MRIANLKKAVWGLMAFASTLVCVMDCYPLIPAVYGVYCLSSGHT
                     IIFYIGLIIGMGYFISIPSICKYLFIIAVIYFGERLFVRKSSKNGCLTTAVVAA"
     gene            complement(1835..2128)
                     /locus_tag="DHV81_05315"
     CDS             complement(1835..2128)
                     /locus_tag="DHV81_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-division initiation protein"
                     /protein_id="HCI93901.1"
                     /translation="MSKRRAKRRGGTLSVSIIVIAFLVVMVVQVVQLKQKESTYAAQK
                     AELEKQYTKESERADEIESMQQYMQSDQYIEDIAKSKLGLAYDNEIIFKEKDK"
     gene            complement(2146..2430)
                     /gene="yabP"
                     /locus_tag="DHV81_05320"
     CDS             complement(2146..2430)
                     /gene="yabP"
                     /locus_tag="DHV81_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HCI93902.1"
                     /translation="MEEISGTKSHKLILNNRRTGSFTGILDVLSFDVGEILLETELGM
                     LDLKGKDLHVNRLNLEKGEADIEGEIDMITYSKVPEVTGKKDKMFGRIFR"
     gene            complement(2540..2779)
                     /locus_tag="DHV81_05325"
     CDS             complement(2540..2779)
                     /locus_tag="DHV81_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCI93903.1"
                     /translation="MRLDKFLKVSRLIKRRTVANEACDAGRVTVNGRPAKASVNVKVG
                     DIIEIAFGQKTVKVKVLNLLDTTKKEEAKDLFEYI"
     gene            complement(2909..3181)
                     /locus_tag="DHV81_05330"
     CDS             complement(2909..3181)
                     /locus_tag="DHV81_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCI93904.1"
                     /translation="MNKADLVAAMAEKAGVSKKDAEASLKAFTDVVAEELKKGEKIQL
                     VGFGTFEVSERAARTGRNPQTGAEMTIAASKAPKFKAGKALKDSLN"
     gene            complement(3335..4669)
                     /locus_tag="DHV81_05335"
     CDS             complement(3335..4669)
                     /locus_tag="DHV81_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HCI93905.1"
                     /translation="MKKFKRLFCIVCMLVLSLAACGKQDTDDGVKKFYNSMNDTTEAV
                     GESTQSGLKAAAEENGKSVKKSSDILIVSDINSANETIRVYNYSTGVQYQYYYGLTTG
                     FFDKYGNHMSVSDIHQGDVVDISGADSDGKAKRIQKSDKVWTNDAVTNFSVDKNKSVL
                     EIGNSSYRLGERTMIFSGSDVIDTDSLTAQDKLAVVGIDKDIVSISVTTGHGTLQLSN
                     TSLFEGSFLQLGDRIFAEITKDMSLDVPEGSYTLAVANNGWGGSTDIEIKRGETTKVN
                     LNDLKGEGPKKSSILFEVDVQGAKIYVDGSEIDYTSPVEITYGKHTLKVTADGYDTWT
                     RTLYVNSKEATIQITINDDTDSSANDSSGTKTNSTGSSQATAQTPSETASERADEKDN
                     QSTSQGSTTGSSQSTNSSRGTNNKSSDSSKNSLTNKDISDYLSTLTSLLSSK"
     gene            complement(4774..5742)
                     /locus_tag="DHV81_05340"
     CDS             complement(4774..5742)
                     /locus_tag="DHV81_05340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93906.1"
                     /translation="MYNQPEVALEKYELTVSRIIKGRGAYICDTGIGQKLLVPYRGHE
                     QRAGTLRDTLAFIQRNGMDVEQISQTKEQCIISRDNCEDAYILKDYRAGRECSTDSID
                     DMRGGSRALAQLHNILEKYPLPSDIEVESLHEKAQRKCAQIVKLKNYILRRSKTNAFE
                     HLFYECYERFLEQGQKSAEILCELENSKTSVPIYCHGAFNQHNVVYTQSGRWLPVNFE
                     TMHPGYPETDLAEYMRKMLEKNHWDTAVADAILDSYCSVRSLDDTSMRLLQALLLFPE
                     KFCKLCNHYSNSRKSWVCDRDVDKLAQLIQLSGEREEYLHALHAIV"
     gene            complement(5796..7136)
                     /locus_tag="DHV81_05345"
     CDS             complement(5796..7136)
                     /locus_tag="DHV81_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCI93907.1"
                     /translation="MRIDLPQNVNFIIDTLYEHGFEAYAVGGCVRDSLLGRTPQDWDI
                     TTSAKPAQVKAIFDHTIDTGIQHGTVTVMLEHVGYEVTTYRIDGEYEDARHPKEVTFT
                     SNLKLDLERRDFTINAMAYNHRAGLVDEFDGIGDLKAHVIRCVGCAHDRFSEDALRMF
                     RAVRFAAQLGFDIEAQTKAAIKELAPALSKVSAERIQVELVKLLTSDHPQMMRDLYEL
                     GLTAVFMPEFDVMMQTPQHNKHHMYSVGEHTLHTLEHVRADKILRLTMLLHDVAKPVC
                     ITTDEEGQNHFKKHPVVGADMTRKILRRLKFDNRTTDCCCKLVKEHDDRPAITERNVR
                     RAMSRIGTELFPLLFEVKRADTLGQSMYKRAEKLEYIAEYERVYRKILADHQCVSKKE
                     MKINGSDLIKMGVEPGPKLGDILDRLYEQVLDDPSLNEAQKLKELANKIITSLI"
     gene            complement(7152..>7652)
                     /locus_tag="DHV81_05350"
     CDS             complement(7152..>7652)
                     /locus_tag="DHV81_05350"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741431.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCI93908.1"
                     /translation="KDKLSKDFRVKIDATDKTPGWKFAAQEVQGISTRIEIGPKDIEK
                     NQCVIVRRDTREKIVVSLDEVNEKLAEVLETMQNDMLEKAKAFLASHINDAHDYNEFK
                     AIAETKPGFIRAMWCGDEACENKIKEDTTVTSRCMPFGDQEQISDVCVCCGKPAHKLV
                     YWGKAY"
BASE COUNT         1739 a         1993 c         1406 g         2378 t
ORIGIN      
        1 gacgcctgcg atgatatcca taagtttccc ctgtctgtcc tttacatgca gatattctag
       61 cacaccatcc gtcaccatga caaggaaatc gccactttga agtgtatttt ttgaatgttt
      121 gctctcctgt gcggcatcga cccccgccgg gagtgattca ttttctataa cacttacttt
      181 tttccctgat ttgataaatg acggtgcagc gcctgttttg gccagctcaa gctcacctgt
      241 gtacatgtct atcgtggcaa aatcaaccgt ggaataggac tcctcgcccg ccgatatgac
      301 catggcagag ttcatcagct ttagggcact ttccctgcta aagccggcct ccaaaagctt
      361 ttcaagcaaa tccaccacca gcgtgctctc ggcctgcgcc tgcttgcccg agcccatgcc
      421 atcggataca catacatgat acatgccatc atccagctga aacatgctgc agctgtcgcc
      481 atttgcctga gagccggtct tctttttggt cgccaccccc gaaagtgcat agaactgatt
      541 ctcctcgtaa agcacaatag atgtcctctc atcggatatt attgaaactg tcccatgtgc
      601 ctgcctgagc ttcactccca tggcgcgtga aaccgcctgc acatactttg aaatttcaat
      661 cccaccatct atcttggtgc agacatctgc ctttatacat acctgctgcc ttgcgttctc
      721 ataaatatgg gcattttcca cctgtatacc agcctctttt gtatatacat aaactcttga
      781 aagacgcagc cttctttttt tatcaataca tttttcagcc ctgcaccagc tatccatgag
      841 ctctgccata gcgtcaagct gtgcggctat gacgcgtctg ttttctgtga gacgcctgta
      901 ccaggcttca tttagctcaa tacgggcaaa tgcctccgtg gcggcttcca ccatgcgcgt
      961 atagtgcggg cagccatcca catatctgtt ttgcacaatt tcctctgttt tcatacgttt
     1021 gcgcactgca tcagcaagca tttttatact gtcggaaatc gaagccccgc ttgtccagca
     1081 cacactgcag ccctcacagc atgcacaaag tctccctgtc acctcaagct gtattttctc
     1141 actctcatca gggattttat ctgccgtaca ttttacagcc atgacatttg cagtcgaaat
     1201 aatgcctgaa agtcctgaaa ctgctgtggc aaatgcctcc tccctgtcgg gcagaagtcc
     1261 tgctatgacc gggttttcat tgtgctcaag agcccttaaa tattcgcatg ctctgcaaag
     1321 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnngct gctacaacgg ctgttgtcag acatccgttt ttactgcttt
     1501 ttctcacaaa gagcctttcc ccaaagtaaa tcaccgcaat aataaagaga tacttacata
     1561 tcgacggtat cgatataaag tatcccattc caataattaa tcctatgtaa aaaataatcg
     1621 tatgtccact actcagacaa taaactccat acactgcagg tatcagcgga taacaatcca
     1681 tcacacacac cagtgtggat gcaaatgcca taagccccca cacagccttt tttaaatttg
     1741 caatcctcat ctttttgcct ccatcttttt tcaacgatta tatgtggtca aaaatgaagg
     1801 ttttggcgaa aaagcgcaac tatatcttca aaaattattt gtccttttcc ttgaaaatta
     1861 tttcattgtc atacgcaagc cccagcttgc tctttgctat atcctctatg tactggtcac
     1921 tctgcatata ctgctgcatg ctttctatct catcagcacg ctccgattct ttagtgtact
     1981 gcttttcaag ctccgctttt tgtgcggcat atgtagattc cttctgcttt agctgcacaa
     2041 cctggactac cataaccaca agaaatgcta taactatgat ggaaacactt aaagttccgc
     2101 cccttctctt tgcacgacgt ttactcatta ttcctcctta ggtgcttatc tgaatatcct
     2161 gccaaacatt ttatctttct ttccggtcac ctccggtacc ttcgaatacg taatcatgtc
     2221 tatctcacct tctatatcgg cttctccctt ctcaaggttt agcctgttta cgtgaagatc
     2281 cttgcccttt aggtcaagca tgcccagctc cgtctccaaa agtatctctc ccacatcaaa
     2341 ggagagcaca tccaaaatgc ctgtaaagct gcctgttctg cgattattca agataagctt
     2401 atgactcttt gtgcctgata tttcttccat ttcctgtccc tcacataatt tttaatatgc
     2461 tccatagaca gccgcacata tacatgccat ctgtggaatc gcatatgtaa atatatgccg
     2521 ggattcatga aaatataacc tatatatact caaaaagatc ttttgcttct tccttctttg
     2581 ttgtgtcaag gagatttaac accttaacct tgactgtctt ttgtccaaat gctatctcta
     2641 ttatgtctcc gaccttgaca tttacagatg cctttgccgg tctgccgttt actgtgacac
     2701 ggcctgcgtc acatgcctca tttgccactg tacgtctctt tatcagacgc gataccttca
     2761 aaaatttgtc taatctcatt gttacctctg tgtcttacct ataataaatc aattcataac
     2821 cattaccctg atttcagtgg atatagccaa aaatgggcgg agccttaaag gcttcaccca
     2881 ttcttctact ccgtgatagt ctaacttatt agttaagaga atcttttaaa gcctttccag
     2941 ccttgaactt aggagcctta gatgctgcaa ttgtcatctc agcgccagtc tgtggatttc
     3001 ttcctgttct tgctgctctc tcagatacct cgaaagtacc gaatccaact aactggatct
     3061 tctcaccttt ctttaactcc tctgcaacaa catctgtgaa tgcctttaaa gatgcctctg
     3121 catccttctt tgatactccg gccttctctg ccatagctgc aactaaatct gctttgttca
     3181 tgataaaatt cctcctctgg attttcataa tttatataat tcgagctgta ttcccaaatt
     3241 acctacaaga atatatatac ttgtttttat ggggtttgtc aaggaaaatg cgctatttcc
     3301 taggctttta tgtactttta ccattcttaa aaccttactt tgaacttaat aaagaggtta
     3361 atgttgaaag ataatcggat atgtccttat ttgtcagact atttttggaa ctgtcagagg
     3421 atttattgtt ggttccccgt gatgaatttg tactctgcga gctgccggtg gtgctgccct
     3481 gagatgtact ttgattgtcc ttctcatcag ccctctctga ggctgtctcg gaaggtgtct
     3541 gtgcggtggc ttgtgaactg ccggtgctgt tagttttggt acctgatgaa tcgtttgcag
     3601 agctgtccgt atcgtcattt atcgtaatct gtatggtggc ctcctttgag ttcacgtaaa
     3661 gcgtcctcgt ccaggtgtca tagccatcgg ctgttacctt aagcgtgtgt tttccgtatg
     3721 ttatctcaac cgggctcgta tagtcgattt cactgccatc gacatatatt tttgctccct
     3781 gcacatcaac ctcgaaaagg atactgctct ttttaggacc ctcacccttt aaatcattta
     3841 aatttacctt tgtagtctct cctcttttta tctcaatatc cgtgctgcct ccccagccgt
     3901 tgttggccac agcgagcgta tagctgccct ccggcacatc aagcgacata tccttagtga
     3961 tttctgcaaa tatcctgtct ccgagctgca aaaagctgcc ctcaaacagc gatgtattgc
     4021 taagctgcag tgtgccgtgc cctgttgtga cagaaattga cactatatcc ttgtcaatcc
     4081 cgacaacagc cagcttatcc tgcgccgtga ggctgtcagt atctatgaca tccgagcctg
     4141 aaaatatcat ggtgcgctca cccagcctgt agctgctatt tccgatttca agcacgctct
     4201 tattcttatc cacagaaaaa ttggtcactg cgtcgtttgt ccacacctta tcggattttt
     4261 gtatacgctt tgccttgccg tctgaatctg cccctgatat gtcaaccaca tcaccctgat
     4321 gtatgtctga aactgacatg tgattgccat atttgtcaaa aaagccggtg gtcagcccat
     4381 agtaatactg atactgcact cccgtcgagt aattgtatac cctgattgtc tcattggcac
     4441 tgtttatgtc cgatactatg agaatatctg aacttttctt aacggacttt ccgttttcct
     4501 ccgcagcagc tttcaaaccg gactgcgtgc tttctcccac ggcctccgtc gtatcattca
     4561 tgctgttata gaattttttt acaccatcat ccgtgtcctg ttttccacat gctgcaagtg
     4621 acagcacaag catgcatact atgcaaaata atcttttaaa tttcttcatc tgtatttcct
     4681 ttaatagctg gctttaaacg tttttctaca ataataatat atcgtatagt catttacttt
     4741 tcaattaaat tcactatata tatgcattac tgcttacact atggcatgca gggcatgcaa
     4801 atactcctcg cgctccccgc ttagctgtat gagctgtgca agcttatcca catcacggtc
     4861 gcatacccat gacttgcgcg agttgctata gtgattgcag agcttacaaa atttttcagg
     4921 aaacaaaagc agtgcttgaa gcagtctcat tgatgtatca tcaagacttc ttacgctgca
     4981 atagctgtca agaattgcat ctgcaaccgc tgtatcccag tgattttttt cgagcatctt
     5041 gcgcatgtac tccgcaagat cagtttccgg atatcccgga tgcatggtct cgaaattgac
     5101 agggagccat cttccggact gtgtatacac gacattgtgc tggttgaagg ctccgtgaca
     5161 gtatatcgga accgatgttt tactattttc cagctcacac aatatttccg ccgatttctg
     5221 cccctgctct aagaatctct catagcattc ataaaacaga tgctcaaaag catttgtctt
     5281 gctgcgacgc agtatgtagt ttttaagctt tactatctgc gcacattttc gctgagcttt
     5341 ttcgtgcagg ctttctacct cgatatccga aggcagagga tacttttcca atatattgtg
     5401 cagctgcgct aatgccctgc tgccgccacg catatcatct atgctgtccg tgctgcactc
     5461 tctgcctgct ctgtaatcct tcaatatgta tgcgtcctca cagttatctc gcgatatgat
     5521 acactgctcc tttgtctgtg atatctgctc cacatccata ccgtttctct ggataaatgc
     5581 cagcgtatcc cgcagggtgc ctgccctttg ctcatgccct ctgtacggca cgagcagctt
     5641 ctgcccgatg cctgtatcac atatatatgc tcctctgccc tttattatcc tgcttactgt
     5701 aagctcatat ttttccaaag ccacttccgg ctgattatac aaaaaaatcc cccctcatat
     5761 gattaatgca tcataactat tcagatgctt tttaatcata tgagagaggt tattatttta
     5821 ttcgcaagct ctttaagttt ttgtgcttca ttgagcgatg ggtcatcaag cacctgctca
     5881 taaagcctgt ccaggatatc gccaagcttc ggtcccggct ctacccccat tttaatcagg
     5941 tcgctgccat ttattttcat ttcctttttt gatacacact ggtggtctgc gaggattttc
     6001 ctgtatacac gctcatattc tgcgatgtat tcaagctttt ctgcgcgctt atacatgctc
     6061 tgtccgagtg tatcggctct tttcacctca aacaacagcg gaaaaagctc tgttcctatg
     6121 cggctcatgg cacgccttac attgcgttcc gtgatggccg gacggtcatc atgctctttt
     6181 acgagcttac agcaacagtc tgtagtcctg ttatcaaact taaggcgcct gagtatcttt
     6241 cttgtcatat cagctcccac aaccggatgc ttcttgaaat gattctggcc ttcctcgtct
     6301 gtcgtaatgc acaccggctt tgccacatca tgaagcagca tggtaagcct taggattttg
     6361 tccgctcgca catgctccaa tgtatgaagc gtatgctctc cgacactgta catatggtgc
     6421 ttattgtgct gaggtgtctg catcatcaca tcaaactccg gcatgaatac cgctgtgagt
     6481 ccaagctcat acagatctct catcatctgc ggatggtctg atgtcagaag cttcacaagc
     6541 tccacctgga ttcgctctgc gctgaccttt gacaatgcag gggcaagctc ttttatggct
     6601 gctttagtct gtgcctctat atcaaagcca agctgggctg caaatcgcac cgcacgaaac
     6661 atcctgagtg catcctcact gaacctgtcg tgcgcacaac ccacacatct gatcacatgt
     6721 gcttttaaat ctcctatgcc gtcaaattca tcgacaagcc ctgccctgtg attatacgcc
     6781 atggcattga ttgtgaagtc gcgtctctcc agatcaagct tcagattgga ggtaaaggta
     6841 acctcctttg gatggcgtgc atcctcgtac tctccgtcta tcctgtaggt ggtgacctca
     6901 tagcccacat gctccagcat gaccgtgact gtgccatgct ggatacctgt gtctatcgta
     6961 tggtcaaata tcgctttgac ctgcgcaggc tttgcagatg tggtgatatc ccagtcctgc
     7021 ggtgtcctgc caaggagact gtcacgcacg catccaccta ccgcatatgc ctcaaagcca
     7081 tgctcgtaga gcgtgtctat tatgaagttg acattctgcg gtaaatctat gcgcatcgtt
     7141 ggattctcct tttaatatgc cttgccccag tatacgagct tatgcgccgg ctttccacag
     7201 catacgcata cgtcggaaat ctgctcctgg tcaccaaatg gcatacaacg gcttgtaact
     7261 gttgtgtcct ctttgatttt gttctcgcag gcctcgtctc cacaccacat agcacggata
     7321 aagcccggct ttgtctctgc gattgccttg aactcattgt agtcgtgcgc atcgtttata
     7381 tggcttgcaa ggaatgcctt tgccttctcg agcatgtcat tctgcattgt ctcaagtacc
     7441 tctgcgagct tctcatttac ctcatcgagt gagacaacga ttttctctct tgtgtctcgg
     7501 cggacgataa cgcactggtt tttctcgata tcctttggtc caatctcgat acgtgttgag
     7561 attccctgaa cctcctgtgc tgcgaacttc cagccaggtg tcttgtcggt tgcatcgatt
     7621 ttcacgcgga aatcctttga gagcttgtcc tt
//
LOCUS       DPUF01000150            3730 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8791, whole
            genome shotgun sequence.
ACCESSION   DPUF01000150 DPUF01000000
VERSION     DPUF01000150.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8791"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="DHV81_05355"
     CDS             <1..660
                     /locus_tag="DHV81_05355"
                     /EC_number="1.1.1.272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515499.1"
                     /note="Involved in the metabolism of aromatic amino acids;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCI93909.1"
                     /translation="VAGYSTETVAQHTFALLFYLLEKLRYYDDYVKSEKYVGDTSFTH
                     FSNVFHQISGMTWGIVGLGNIGRRVADIAKAFGCHVVYYSTSGRNSQPGYERVDFDTL
                     LTTSDIVSVHAPLTDDTLGLMDKAAFEKMKKSAIFLNLGRGPIVNEADLAQALMDGEL
                     AAAGLDVLAQEPMSEDNPLRAIKDSNKLIITPHIAWASVEARTNLMHIIYSQIEDFFT
                     N"
     gene            781..2718
                     /locus_tag="DHV81_05360"
     CDS             781..2718
                     /locus_tag="DHV81_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCI93910.1"
                     /translation="MSIKFEITKQNNIHFGIAAAAAALVGYFTSASWWVILLFAAVYF
                     GLVNVKLELPVKLSWLWAAILLAIGAILSVFSVQYVLLTDEDFVKTTDMVCVVNVVLA
                     LAIYLVILFITNNTRLTCTIASIAILAFGFIDYFVYEFRGNEFTYADLKSAGTGLSVV
                     TKYKFVIDYKFLYVILAAVLYIVLVRRIEVQFESAIHMRIISILLTIICVLYVIMNSM
                     SLNTETWEKKGTYRNGYLLNFVLGIRDSFVKAPDGYSKAAVDKIAGNFKETDSSYSQS
                     DAKNPTIIVIMNESFADLSVVGDFETNTQVTPFMDSLSENTLKGYALSSVYGAKTPNS
                     EWEFETGNSMAFLPDGSVVYQQYINDDPTSIVSNLKNIGYTTVAMHPYYATGWSRNKV
                     YPHLGYDETYFIDDFDQTKILREYITDQELYDKIIDRYEKKSDDEKLYIMGVTMQNHG
                     GYGERYDNFNQEVYKVGASYTDANQYLSLLNESDKALENLITYFKGVDDPVEIVFFGD
                     HQPGLCNDFIKLLNGKGNSGLTEQELENLYKVPFFIWTNYETDAQTVDVTSLNYLSTL
                     TLERANIDLPAYNRFLAELMEKIPAINSRGYYSKKNECFMHVDDATGDEAKWIKAYNI
                     LQYNSMFDEKDRSSLLFPYLK"
     gene            2791..>3730
                     /locus_tag="DHV81_05365"
     CDS             2791..>3730
                     /locus_tag="DHV81_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HCI93911.1"
                     /translation="MKLSDLLQFDNIIVQCHDNPDADALASGFGVYEYLRLNGKSPRL
                     VYGGRNIIHKSNLVLMVDSLGIPIEHVDFLDNPQLLVTVDCQYGGGNVTFFKAENVAV
                     IDHHRVSGQLPAMNRVMSYMGSCATIVWDMLREEGVEINRNLATALYYGLYTDTGEFT
                     EITHSLDRDLRDEADFDSTIVAKFRNANMSLEELDIAATALLGRDYIEEYRLAIVKAG
                     ACDPNVLGIISDFVLEVDAIDICMVFSVIKNGVKLSFRSCIKEVSASEMAQEVCRDIG
                     SGGGHYYKAGGFIPMDLLIDSYNVYCQENDVTPRFQY"
BASE COUNT         1083 a          691 c          975 g          981 t
ORIGIN      
        1 gtcgcagggt attctacaga gactgtggcg cagcatacat ttgcactgct attttatctg
       61 cttgagaagc tgagatatta tgatgattat gtgaagagcg agaaatatgt gggtgatacg
      121 tcgtttacgc atttttctaa tgtgtttcac cagattagcg gtatgacctg gggcatcgtg
      181 gggcttggaa atatcggaag acgcgtggct gatatcgcaa aggcttttgg ctgccatgtg
      241 gtttattatt ctacatcagg aagaaattcg cagccgggct atgagagagt ggattttgat
      301 actttgctta ccacgtctga tatcgtgtcg gtgcatgcgc cgctcacaga tgacacactt
      361 ggtctgatgg acaaggctgc gtttgaaaag atgaaaaaat ctgcgatatt tttaaatctt
      421 ggcagaggtc ctatagtgaa tgaagcggat ctggcacagg cgcttatgga tggagagctg
      481 gcggcggcag gtcttgatgt gctggcgcag gagcctatga gtgaggacaa tccgctccgt
      541 gcgataaagg attcaaacaa gctgattatc acaccacata ttgcgtgggc aagcgttgag
      601 gcaagaacaa atctgatgca cattatatac agtcaaattg aggacttctt tactaattag
      661 gaaatataat ttaaaatcaa atcgcaacat taagaggcag cattaagaga aagcattaag
      721 agaaagtctt aagaggaagc attaagaggc aacattaaga agcattatta agaggcagaa
      781 atgagcatca aatttgagat aacaaaacaa aacaacatac attttggaat tgcagcagca
      841 gcggccgcgc ttgtaggtta ctttacatcg gcgagctggt gggtgattct tttgtttgcg
      901 gcagtatatt ttggacttgt aaatgtgaag cttgagcttc cggtgaagct ttcgtggctt
      961 tgggctgcga tactgcttgc catcggagcg atactatctg ttttttcggt gcagtatgtg
     1021 ctgctgacag atgaggactt tgtgaaaacc accgatatgg tatgcgtggt gaatgtggtt
     1081 ctggcgctcg ccatatatct ggtgatactt tttatcacaa acaacacgag gctgacatgt
     1141 accatagcat caattgccat actcgcgttt ggatttatag attactttgt ctatgaattc
     1201 cgtggcaatg agtttacgta tgcggatttg aagtcagcgg gaacaggact ttccgtcgtg
     1261 acaaaataca aattcgtcat agactacaag tttttgtatg tgattctggc ggctgttttg
     1321 tatatagtgc tggtaaggcg tatagaagtg cagtttgagt cggctatcca tatgagaatt
     1381 atttcgattc ttctcacaat aatatgcgtg ctgtatgtca tcatgaattc catgtcactg
     1441 aacaccgaga cctgggagaa aaagggcacg tacagaaacg gctatctgct gaactttgtg
     1501 ctcggcataa gggacagctt tgtgaaggca cctgatggct acagcaaggc ggcagttgac
     1561 aagatagcag gcaatttcaa ggaaacagac agctcatatt cgcagtctga tgcaaaaaat
     1621 ccgacaatca ttgttatcat gaatgagtcc tttgcggatt tgtcggtggt gggagatttt
     1681 gagaccaaca cgcaggtcac accgtttatg gattctctga gtgaaaatac gcttaaaggc
     1741 tatgctcttt cgtccgtgta cggtgcgaag acgccaaact ctgagtggga atttgagaca
     1801 ggaaactcga tggcatttct gccggacggc tcggtggttt atcagcagta tataaatgat
     1861 gacccgacat ccatagtttc aaacctgaag aatatcggct atacgacggt ggcgatgcat
     1921 ccgtactatg cgacaggctg gagcagaaac aaggtttatc cgcatctggg ctatgatgag
     1981 acttacttta tagatgattt tgaccagaca aagatactca gggaatatat cactgaccag
     2041 gagctctatg ataagattat tgaccgctac gagaaaaaga gcgatgacga gaagctttac
     2101 atcatgggag tcacgatgca aaaccacggt ggctacggtg agagatatga caacttcaac
     2161 caggaggttt acaaggttgg agcgtcatac accgatgcga accagtatct gtcacttttg
     2221 aatgagagtg acaaggcact cgaaaatctc atcacatact ttaagggtgt ggatgatccg
     2281 gtagagattg tgttttttgg agaccaccag ccgggattat gcaatgattt tatcaagctt
     2341 ttaaatggca agggcaattc tggacttacg gagcaggaac ttgagaatct gtacaaggtg
     2401 ccgtttttca tatggaccaa ttacgagacg gatgcacaga cagttgatgt gacgagcctc
     2461 aactacctgt ccacactgac gcttgagaga gccaacatag atctgccggc atacaaccgg
     2521 ttcctcgcag agctgatgga gaagattccg gccataaatt caaggggcta ttattcgaaa
     2581 aagaatgagt gctttatgca tgtggatgac gcaacaggag acgaggcaaa atggataaag
     2641 gcatacaata tcctgcagta caattcgatg tttgacgaga aggacagaag ttcactgctg
     2701 tttccgtacc tgaaataatg tgatggtttg aggcagccat aaatacacat atgagaaaat
     2761 gaattaatat aactattcag ggagctaata atgaagctaa gtgatttatt acaatttgat
     2821 aacataattg tgcaatgtca tgataatccg gacgcagatg cactggcaag cggctttgga
     2881 gtctatgaat acctgaggct gaatggaaaa agtcccagac ttgtatacgg aggcaggaat
     2941 atcatacaca agagcaatct ggtgcttatg gtagacagtc ttggcatacc gatagagcat
     3001 gtggattttt tagacaatcc gcagctgctt gtgactgtcg attgtcagta cggtggtggg
     3061 aatgtcacat ttttcaaggc agaaaatgta gctgtcatag atcaccacag agtatcggga
     3121 cagcttcctg ctatgaaccg tgtcatgagc tatatgggct catgtgcgac aattgtgtgg
     3181 gatatgctgc gtgaggaggg cgtagagata aatcgaaacc tggcaacagc actgtattat
     3241 ggactttaca ccgacaccgg agaatttacc gagataacac attcgcttga tagggacctg
     3301 cgcgatgagg cggattttga cagcactata gtggccaaat tccgaaatgc caacatgtca
     3361 cttgaggagc tggacatagc cgcaacagca ttgctggggc gcgattatat agaggaatat
     3421 cgtctggcga tcgtaaaggc aggggcatgt gacccgaatg tactcggcat cataagcgat
     3481 tttgtgctgg aggtggatgc tattgacata tgtatggtat tttcagtgat taagaatgga
     3541 gtgaagcttt cgtttagaag ctgcataaaa gaggtgagcg caagcgaaat ggcgcaggaa
     3601 gtgtgcaggg acataggctc cggcggagga cattattaca aggccggtgg atttattccg
     3661 atggatctgc tgatagacag ctataatgtc tactgccaag aaaacgacgt gacaccaaga
     3721 tttcagtaca
//
LOCUS       DPUF01000151            4319 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_25257, whole
            genome shotgun sequence.
ACCESSION   DPUF01000151 DPUF01000000
VERSION     DPUF01000151.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_25257"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="DHV81_05370"
     CDS             <1..129
                     /locus_tag="DHV81_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI93912.1"
                     /translation="ERGNTSILDVSLSVGFHNLSYFHRAFKKRYHMTPLSFIKSLE"
     gene            complement(134..>418)
                     /locus_tag="DHV81_05375"
     CDS             complement(134..>418)
                     /locus_tag="DHV81_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HCI93913.1"
                     /translation="EVTFEGDYPAWEYKKDSKLREDMVRIFEEMYGKKPTVEAIHAGV
                     ECGLLSGKIEGLDCISMGPDMYDIHTTEERLSISSAKRSYEYILRVIACK"
     assembly_gap    419..566
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<567..1588)
                     /locus_tag="DHV81_05380"
     CDS             complement(<567..1588)
                     /locus_tag="DHV81_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HCI93914.1"
                     /translation="MRVLTDLEPKNVFSFFEDICSIPHPSYKEEKISNYLVEFAKARG
                     LEHYQDELHNVIIIKEASEGYEDVEPIILQGHMDMVCEKAPDCDKDMDEEGLDLEIDG
                     DFISAKGTTLGGDDGIAVAYALAILDDDSLSHPRLEFVCTVCEEVGMEGATGIDVSML
                     KGKKLLNMDSEEEGVMLASCAGGCRADVKLPVERETVSGTKLTVSLSGLTGGHSGSEI
                     DKGRGNANALMSRLLRDASAPVAIASIDGGRKDNAIPRECTAQIIVAPDKAAAVKAAI
                     EQAADEIAEEFKASDPDLKLTLSEETDCSEPAISAKDSARVIALIEALPNGIIRMSRE
                     IDKLVET"
     gene            complement(1752..2360)
                     /locus_tag="DHV81_05385"
     CDS             complement(1752..2360)
                     /locus_tag="DHV81_05385"
                     /inference="COORDINATES: protein motif:HMM:PF13346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93915.1"
                     /translation="MFALIKKDIIFTKKWILLSVVYAVVATWLIMHEGATNILFTFFL
                     VPFFVTSLPFTKIMSIEDNSDTRDFIRRLPVSKYLVVFSRAVFIIFMLLISSISLFVI
                     QTIGFNVEYNTDMLIKIASIILIFLLYFMLQLGVFYKYSYHASQSILVIISFAVIVLG
                     FITENIDLVKYFKNFDWSILLFIMAIINVVVYLIDTKLYAGK"
     gene            complement(2362..3195)
                     /locus_tag="DHV81_05390"
     CDS             complement(2362..3195)
                     /locus_tag="DHV81_05390"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI93916.1"
                     /translation="MIKGLNISKKYKKFELQPTSIEIDKGEIVALIGENGAGKSTLIK
                     CMCGVIDADEGQLEVDDQVVKKLDDVLVLGFMNQDQNVYNEIKIRDLTNFVKDVMKKK
                     FDQEVYYYYFKDVFQLDEDYKMKELSTGMKVKYFLSLELAKNPDLLVLDEPTSGLDPI
                     VRDEVLDILRCVAKEKNTSVLFSSHITEDIEKIADRVIFINNGRIVLQGDKKEIKKKY
                     VKLNQQQYVILVNAGMAKECVSIHDYYIVDRDLAMTCNVEVKEALLSDILSYMRKRSE
                     D"
     gene            complement(3312..4088)
                     /locus_tag="DHV81_05395"
     CDS             complement(3312..4088)
                     /locus_tag="DHV81_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCI93917.1"
                     /translation="MMKPMLEICCGSFSDVKTAYENGADRVELNSALYMGGLTPTLAN
                     LIYAKENCNIPVVAMVRPRGGGFCYSDEEYETMLMDAKILLEHGADGIAFGFLTEEKM
                     LDKKRTKEMIELIHKYGREAVFHRAFDCIDNQDSAAEKLIRLGADRILTSGGAVNVWD
                     GRKQLKHLQNQYGKDITILAGSGVNDTNARELIEYTGITQVHSSCGSWTCDVTAAGNA
                     VDFSYDEAMKNCYQCADAGKVRKLAEDVSREDRICSDLHL"
     gene            complement(4179..>4319)
                     /locus_tag="DHV81_05400"
     CDS             complement(4179..>4319)
                     /locus_tag="DHV81_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCI93918.1"
                     /translation="MTRQIYERTKITSTEREMKDFYNKYVKSLRLKINEDGVFEIVVN
                     EY"
BASE COUNT         1207 a         1004 c          593 g         1367 t
ORIGIN      
        1 gagcgcggca acacctcaat tttagatgtc tcactgtctg tcggatttca caatctaagc
       61 tattttcaca gggcatttaa aaagaggtat catatgacac ctctttcatt tatcaaaagc
      121 cttgaataaa tatttatttg cacgcaatca ctctgagtat atactcatat gacctctttg
      181 cagaggatat ggaaagcctc tcctctgtcg tatggatatc atacatgtcc ggtcccattg
      241 atatgcagtc gagcccctca atcttgccac ttaaaagtcc gcactcgaca ccggcatgaa
      301 tagcctcaac tgtaggcttt ttgccataca tctcctcaaa aatccgtacc atatcctctc
      361 gtagctttga gtccttcttg tactcccatg cagggtaatc gccctcaaat gtaacctcnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnngtct caacaagctt atcaatctcc ctgctcattc
      601 tgatgatgcc gtttggcaga gcctcaataa gtgcgataac tctcgcgctg tccttggcac
      661 ttatagcagg ctcactgcag tcagtctcct ctgacaaggt aagcttaagg tcaggatcag
      721 atgccttaaa ctcctccgca atctcatctg ctgcctgctc tatcgcagcc ttcactgctg
      781 cagccttatc cggtgctaca ataatctgtg cagtacactc tcttggaatg gcattatcct
      841 ttctgccgcc atctatagaa gctatagcta caggtgccga tgcgtcgcga agcagtctgg
      901 acataagagc attggcattg cccctgccct tatcaatctc tgagcctgag tgtccgccgg
      961 tgagacctga taagctcaca gtgagctttg ttccgctcac tgtctctctc tctacaggca
     1021 gcttcacatc agctctgcat ccaccggcgc agcttgcgag catcacaccc tcttcctcgc
     1081 tgtccatatt aagaagcttc tttcccttaa gcatagacac atcgatgcct gtagcaccct
     1141 ccatgcctac ctcctcgcat acagtgcata caaactcaag gcgtggatgc gacaggctgt
     1201 catcatctaa aatagcaagc gcatatgcca ccgcgatacc atcatctcca ccgagtgtag
     1261 tgccctttgc cgagataaaa tcaccatcta tctcaaggtc taagccttcc tcgtccatat
     1321 ccttatcaca gtcaggtgcc ttctcgcaaa ccatatccat gtgtccctgc agtatgattg
     1381 gctccacatc ctcatatcct tctgatgctt cctttatgat tatcacattg tgaagctcat
     1441 cctgataatg ctcaaggcct cttgcctttg caaactccac cagataatta ctgattttct
     1501 cttccttgta tgacggatga gggatactgc atatatcctc gaaaaacgaa aaaacgttct
     1561 ttggctctaa atctgttaat actctcattt tactctctcc ttaaaaaaag tctgccggcg
     1621 aagctgccga cagacctatt ataacacaat ccggtattta attgtatatg tattattcaa
     1681 gtccaaatct tctgatatta ttttattcct atattctgat gctcagtaaa gtaatcgagc
     1741 catacttata attattttcc tgcatacaat ttggtatcaa taagataaac taccacattt
     1801 attatagcca taataaataa caatatcgac caatcaaaat tcttaaaata tttcactaaa
     1861 tctatatttt ctgttataaa cccaagcact attacagcaa aggaaataat taccaggatt
     1921 gattgtgatg catgatatga atatttataa aaaacaccta attgaagcat aaaatacaac
     1981 aaaaaaatca aaattatgga agctattttt attagcatat ctgtattata ttctacatta
     2041 aagccaattg tttgtataac aaataagctt attgaagaaa ttaataacat aaaaataata
     2101 aacactgctc tcgagaatac aactaaatat tttgataccg gtaatcttcg aataaaatca
     2161 cgtgtatcac tgttatcctc aatactcata attttagtaa atggaagtga tgttacaaaa
     2221 aaaggcacta aaaaaaatgt gaacaaaata tttgtagcgc cttcatgcat tataagccag
     2281 gtggccacca ctgcataaac tacagataat agaatccatt tttttgtaaa tataatatct
     2341 tttttaatta atgcaaacat attagtcctc actcctcttt ctcatatatg aaagtatatc
     2401 gcttaataaa gcttccttta cttctacatt gcaagtcatt gccaaatcac gatccacaat
     2461 atagtaatca tggattgaaa cacattcttt tgccatccct gcattcacaa gaataacata
     2521 ctgttgctga tttaacttga catatttttt ctttatttct tttttatcac cttgtaatac
     2581 aatacgtcca ttatttatga aaataactct atctgctatt ttttcgatat cttctgtaat
     2641 atgggacgag aataacacgg aagtattttt ttcttttgca acacatctta gaatatctaa
     2701 gacttcatca cgaacaattg gatctaatcc tgaggttggt tcatcaagta ccaataaatc
     2761 tggatttttt gcgagttcta aactcaaaaa atatttaacc ttcattcctg ttgaaagttc
     2821 tttcatctta taatcttcat ccaactgaaa tacatcttta aaatagtaat aatatacttc
     2881 ctgatcaaat tttttcttca tcacatcttt tacaaaattc gtcaaatctc gaattttgat
     2941 ttcattgtaa acattttgat cctgattcat aaatccaaga accaaaacat cgtctaattt
     3001 ttttactacc tgatcatcta cttcaagttg tccctcatct gcatcaatta ctccacacat
     3061 gcacttgatt aatgttgatt tacctgctcc attttctcca atcaatgcaa caatttcacc
     3121 tttgtcaatc tcaattgatg taggctgtaa ttcaaatttt ttatactttt tgctaatatt
     3181 aagtccttta atcatgtctc tgtttcctta tatatctgta ctatttgttt gtaattggat
     3241 agagtataac ataaatcttg tattatcgta caatcaaaca gtttattatt tttatatttc
     3301 atctctaatc atcataagtg caaatccgag cagattctgt cctctctact tacgtcttcc
     3361 gcaagcttcc taaccttccc tgcatccgca cactgatagc agttcttcat cgcttcatca
     3421 tacgaaaaat ccactgcatt acccgcagct gtcacatcac acgtccatga gccacacgag
     3481 ctgtgcacct gcgtaatacc tgtgtattcg ataagctctc tcgcattggt atcattcacg
     3541 ccacttccgg ccagaatagt aatatctttt ccatattgat tttgaagatg cttaagctgt
     3601 tttctgccat cccatacatt tactgctcca ccgcttgtga ggatacgatc tgcgcctagc
     3661 ctaataagct tctccgctgc actgtcctga ttatctatgc agtcaaaggc gcggtgaaac
     3721 acagcttccc tgccatactt atgaatgagc tctatcattt ccttcgtgcg ctttttgtca
     3781 agcatttttt cctctgtcag aaatccaaac gcaataccgt ctgctccatg ctctaaaaga
     3841 attttggcat ccataagcat agtttcgtat tcttcatccg aatagcaaaa tccgccgccc
     3901 ctcggacgaa ccattgccac taccggtata ttgcagttct cctttgcata tattaaattg
     3961 gcaagtgtag gtgtcagccc tcccatatat agtgcactgt tcagttcaac cctgtctgca
     4021 ccattttcat atgctgtttt cacatccgaa aagctgccac agcagatttc aagcatcggt
     4081 ttcatcatac tttcccactc tttccgctaa aataaggctt aaccttcaca caatacatgt
     4141 gtgaccgaca ataattacat aaaacagttt taagataatc aatattcatt gaccacaatc
     4201 tcgaatactc cgtcctcatt aatttttaac ctcaatgact taacatattt attgtaaaaa
     4261 tccttcattt ctcgctctgt cgatgtaatt ttagtcctct cgtatatctg tcttgtcat
//
LOCUS       DPUF01000152            2969 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_9622, whole
            genome shotgun sequence.
ACCESSION   DPUF01000152 DPUF01000000
VERSION     DPUF01000152.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_9622"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="DHV81_05405"
     CDS             <1..549
                     /locus_tag="DHV81_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase"
                     /protein_id="HCI93919.1"
                     /translation="VKATFFVTADTSGKYDDVYKRIVQDGHTIAMASYSNSYSKIYES
                     TEAFTDDLTQISDYITNLTGIAPDIYRFPGGSMNQISNVDMVELVKILNSKHITYFDW
                     NVNSGDTADNCSVDDIVNNVTSGIAKYDNSVVLLHDDSNRSTTAEAIEPLVESIKAQG
                     AEILPIDNNTYKVQYIKSDTVG"
     gene            573..1376
                     /locus_tag="DHV81_05410"
     CDS             573..1376
                     /locus_tag="DHV81_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HCI93920.1"
                     /translation="MGLFKEFKDDFSQAVNELVPGDVPEKEVEPKAQTADNLVVDTIG
                     ENVDVASELSKLDGLLEHVEEQPQETTVPRPTPSENFAEHTINTQKEEQIPQMNDIPQ
                     PVNTQPVNNNVLSGDVQDETSVITASTVLTGDLQSSGSFDIQGTINGNVMCNGKIVVT
                     GTVNGNTNSSEFFADVAKVEGEIVSTGTVKIGAGSVIIGNISASSAVIAGAVKGDIDV
                     QGPVVVDTSAVIMGNIKSRSVQINNGAVIEGFCSQTYADIDVQGLFEGK"
     gene            1379..2074
                     /gene="pyrH"
                     /locus_tag="DHV81_05415"
     CDS             1379..2074
                     /gene="pyrH"
                     /locus_tag="DHV81_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCI93921.1"
                     /translation="MSRILLKLSGEALAGDKHHGFDEATVIEVAKQVKQIVDNGTQVG
                     IVIGGGNFWRGRTSETIDRTKADQIGMLATVMNCIYVSEIFRYVGMKTAVLTPFECGN
                     MTKLFSKDRANKYFAHDMVVFFAGGTGHPYFSTDTATVLRAIEIEADGIYLAKAIDGV
                     YDSDPKTNPAAKKYDELSIEEVVEKKLAVVDLTASIMCLENKMPMYVFGLNEENSIVN
                     ALTGKFNGTKVTV"
     gene            2108..2662
                     /locus_tag="DHV81_05420"
     CDS             2108..2662
                     /locus_tag="DHV81_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HCI93922.1"
                     /translation="MDSRLNPFQDKMEKTLNNLEEEYAGIRAGRANPHILDKLRVDYY
                     GTPSPIQSVANVSVPEPRMIQIQPWEASMVKEIEKAINCSDIGINPTNDGKLIRLVFP
                     ELTEERRKDLAKDIKKKGEEAKVAVRNIRRDAIDSIKKTGKEEGISEDEIKGLEDDAQ
                     KLTDKFVAKVDAAVDAKCKEILTV"
     gene            2818..>2969
                     /locus_tag="DHV81_05425"
     CDS             2818..>2969
                     /locus_tag="DHV81_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCI93923.1"
                     /translation="MSTPEHIAIILDGNGRWAKSKGMPRNYGHTVGAKNVENICKAAN
                     DLGVKYL"
BASE COUNT          984 a          537 c          678 g          770 t
ORIGIN      
        1 gtcaaggcta ccttctttgt cacagcagat acctccggta aatatgatga tgtttataag
       61 cgcatagtgc aggacggaca cactattgcg atggcttcct acagtaattc atacagtaaa
      121 atttatgaat caacagaggc atttaccgat gacctgacac agatttccga ttatatcacc
      181 aatctgactg gaatagcacc ggatatttat cgttttcccg gtggcagcat gaaccagatt
      241 agtaatgtag acatggttga gcttgtaaag atactcaatt caaagcatat cacatacttt
      301 gactggaatg tcaattctgg tgatacggcc gacaattgca gtgtcgatga tattgtcaat
      361 aatgtcacct caggtattgc caaatatgac aattcagttg tgttattaca tgatgattca
      421 aacagatcaa ccacggcaga ggctattgag ccgcttgttg aaagcataaa agcgcagggt
      481 gccgaaatat tgccgattga taataataca tataaagttc aatatatcaa atcagatact
      541 gttggataag ataataaatg gaggaaatat tcatgggatt attcaaagaa tttaaggatg
      601 atttttctca ggctgtaaac gagcttgtac ccggagatgt acctgagaaa gaagtagagc
      661 caaaggctca gacagctgac aatcttgtag ttgatactat aggagagaat gttgacgttg
      721 catcagagct ttcaaagctc gacggattac ttgagcatgt cgaggagcag ccacaggaga
      781 caactgtacc aagaccaact ccaagtgaaa attttgcaga gcatacaatc aatacacaaa
      841 aagaggagca gataccacaa atgaatgata taccacagcc agtaaataca cagccagtaa
      901 acaataacgt tttatcagga gatgtacagg acgaaacatc agtcatcaca gcaagtactg
      961 tactcacagg agatttacag tcatcaggat catttgatat tcagggaacc atcaacggaa
     1021 atgtaatgtg taacggcaag attgttgtta caggaacagt caacggcaac acaaattcat
     1081 cagagttttt cgctgatgta gctaaggttg agggtgaaat tgtttcaaca ggaactgtaa
     1141 agataggtgc aggatctgtt atcatcggca atatctcagc atcttcagca gttatcgcag
     1201 gagctgtaaa gggcgatata gatgttcagg gacctgttgt agtagatact tctgcagtta
     1261 tcatgggtaa tatcaagtca cgttcagtac agattaacaa cggtgccgtt atcgagggct
     1321 tctgttctca gacatacgct gatatcgatg ttcagggact tttcgagggt aaataaatat
     1381 gagtagaata ttactaaaat taagtggtga agcacttgca ggtgataagc accacggctt
     1441 tgatgaggct acagtcattg aggttgcaaa acaggttaaa cagattgttg ataatggtac
     1501 acaggtaggt attgttatcg gtggtggaaa cttctggaga ggcagaacga gtgagaccat
     1561 cgaccgcaca aaggctgacc agattggtat gctcgctaca gttatgaact gcatttatgt
     1621 atctgagatt ttcagatatg tcggaatgaa gacagctgtt cttactccgt ttgagtgcgg
     1681 caacatgaca aagcttttct ctaaggacag agcaaacaag tatttcgcac atgatatggt
     1741 agttttcttt gccggtggta caggccatcc ttatttctca acagatacag ccacagtgct
     1801 tcgtgcaatt gagattgagg cagatggtat ttaccttgcc aaagcaattg acggagtata
     1861 tgacagtgat ccaaagacca atcctgctgc aaagaagtat gatgagcttt caatcgaaga
     1921 ggttgttgag aagaagctcg cagtagttga cctcacagcg tctatcatgt gccttgagaa
     1981 caagatgcct atgtatgtat tcggactcaa tgaggaaaac agcattgtaa atgctcttac
     2041 aggcaaattt aacggtacaa aggttactgt atagtaaatt gcatataatg catatcagga
     2101 ggataatatg gattcaagat taaacccttt ccaggataaa atggaaaaaa cattaaataa
     2161 tctcgaggaa gagtatgcag gcatccgtgc aggaagagcc aatccacata ttcttgacaa
     2221 gcttcgtgta gattattacg gcacaccttc accaattcag agtgtagcca atgtaagtgt
     2281 tccagagcct agaatgattc agattcagcc atgggaggct tctatggtta aggagattga
     2341 gaaagcaata aactgctctg acatcggaat taatcctaca aacgacggaa agcttatcag
     2401 acttgttttc cctgagctca cagaggagag acgtaaagat cttgcaaagg acatcaagaa
     2461 aaaaggcgag gaagctaagg ttgctgtccg caatatcaga agggatgcaa tcgacagcat
     2521 caagaagacc ggcaaagagg aaggcatttc agaggatgag attaaaggcc tcgaggatga
     2581 cgctcaaaag cttaccgaca agtttgtcgc aaaggttgac gctgcggttg atgcaaaatg
     2641 taaagagata cttactgtat agtatttata tcacgtatac aaacaaggga catgttattc
     2701 gtgtccctta tttgcttatt cccggtttta ctgttcacac ttaactgtgt gaccagcaaa
     2761 taaatgatgc gtcatgattg taaatatata atatgcataa atgaaaggaa ataaaatatg
     2821 agtactccgg aacatatagc aatcattctg gacggaaacg gccgctgggc caaaagtaag
     2881 ggtatgccaa gaaactacgg tcatacagta ggagccaaaa atgttgagaa tatatgtaag
     2941 gctgcaaacg acctcggcgt taaatatct
//
LOCUS       DPUF01000153           10952 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_9621, whole
            genome shotgun sequence.
ACCESSION   DPUF01000153 DPUF01000000
VERSION     DPUF01000153.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_9621"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="DHV81_05430"
     CDS             complement(<1..280)
                     /locus_tag="DHV81_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCI93924.1"
                     /translation="MKITILCVGKVKEKFYRDAIAEYSKRLSRYCKLEITEVSDEKTS
                     EQATQTEIDIVKNKEGERLLKNIKDDAYVFCLAIDGKQLDSVELSQKMD"
     gene            327..818
                     /locus_tag="DHV81_05435"
     CDS             327..818
                     /locus_tag="DHV81_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93925.1"
                     /translation="MKIYVDEQKRYEDTKATGQCFTGVGAIGLIAIVLLDTGVIKLAA
                     LDSVNKLMVSIVMGLVFLIFFIIGMKSFMELKDISKKIDRNNSLEEEIMEYVTVTHRD
                     ELMTLASSGEKEDACSGDLYYKRAELITSVITEKYSLLEESFLDHMVEEIYSKIYSDI
                     VEE"
     gene            860..1438
                     /locus_tag="DHV81_05440"
     CDS             860..1438
                     /locus_tag="DHV81_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCI93926.1"
                     /translation="MKELQDRILKDGQVIGTNILKVNKFINHQVDPVLMEHIGEDFAN
                     HFAGKGITKVVTIESSGIAPALMAAAKMNVPLVILKKQPSKTLHNDLYQTQVTSFTTE
                     KSYELTLSRDVISEDDNILLIDDFMADGEAATGAIRLLRMAHATVAGIGILIEKSFQP
                     GRRKINEQGYEVYSLARIKYMDEYQIDFIEEN"
     gene            complement(1573..6159)
                     /locus_tag="DHV81_05445"
                     /pseudo
     CDS             complement(1573..6159)
                     /locus_tag="DHV81_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742606.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
     assembly_gap    2977..3091
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6162..7249)
                     /locus_tag="DHV81_05450"
                     /pseudo
     CDS             complement(6162..7249)
                     /locus_tag="DHV81_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742607.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
     assembly_gap    6720..6755
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7276..8313)
                     /gene="rseP"
                     /locus_tag="DHV81_05455"
     CDS             complement(7276..8313)
                     /gene="rseP"
                     /locus_tag="DHV81_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCI93927.1"
                     /translation="MSIIIAIILFSIIVIFHELGHFWLAKANGIKVNEFCLGLGPTLF
                     GVQRGETKYSIKLLPFGGACIMEGEDESSGDDRAFNNKSVWARISVVFAGPFFNFIMA
                     FLFALIIICSVGYDSPKLAGVIEGYAAEEAGIKAGDEIVKLNNTNIHFYREISLYSML
                     HEGETVDVTYLRDGKKHTTTLKPKYDETTKRYLYGFNVSGERTKPGLGKALLYSCYEV
                     KYNIYTTIEGLKMLCTGAASVNNLSGPVGIVKNMGDTYEQAVSMSGVWLGILNMLNWG
                     VLISANLGVMNLLPLPALDGGRLVFLIVEAIRRKRVDPEKEGYVHLVGIVLLLLLMVV
                     VMFNDIRKLIV"
     gene            complement(8325..9467)
                     /locus_tag="DHV81_05460"
     CDS             complement(8325..9467)
                     /locus_tag="DHV81_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCI93928.1"
                     /translation="MKKISILGSTGSIGTQTLDVIREHGDMQVVALSCGRNLSLIEKQ
                     AREFKPQFVSVSDENDAKKLRTSLADTDIEVGYGMDGLIRCATIEPCDIVVTAIVGML
                     GIRPTIAAIKAKKTIALANKETLVTAGHIIIPLAKEYGVSILPVDSEHSAIFQSLQGN
                     SMNPIKKILLTASGGPFRGRKLSELEGIRVEDALKHPNWSMGQKITIDSSTMVNKGLE
                     VIEAKWLFDVDLSQIQVVVHPQSVIHSAVEYADGAVIAQLGTPDMRIPIQYALYYPSR
                     PALSGDRLDLFKLKDLTFEAPDLDTFKGLALAMKAARAGGNIPTAFNAANERAVALFL
                     NKKIKYLEIIDIIEACMENASFIENPSVDEILDTEQCAYDYISKRW"
     gene            complement(9553..10356)
                     /locus_tag="DHV81_05465"
     CDS             complement(9553..10356)
                     /locus_tag="DHV81_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCI93929.1"
                     /translation="MFKTRLLSGIVLVIAAIFLIVTGNEVLLFSTLLISCIGMFELFR
                     VFKFEKSLLSVVPYIALILFYINLRWSFIPDVMMLFMGFLIVLMFVYVLSYPKYDAKQ
                     LMAAFFGMFYVGTMLSYVYQIRTLNNGLYLAFLVFLCSWGCDTCAYCVGVLIGKHKMA
                     PKLSPKKSIEGAVGGVLGSALLTALYCFIFRNHMNIDGTEIAILAVIAAVAGLISMVG
                     DLCASAIKRNYDIKDYGHLIPGHGGILDRFDSMIITAPIIYYLASFFLS"
     gene            complement(10356..>10952)
                     /gene="uppS"
                     /locus_tag="DHV81_05470"
     CDS             complement(10356..>10952)
                     /gene="uppS"
                     /locus_tag="DHV81_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HCI93930.1"
                     /translation="NDLGVKYLTLYAFSTENWNRPESEVAALMKLLESYLKNCIKTAN
                     KNNMRVRVIGEISRLSENFQEKIRNLEEVSSVNTGLNLTIAINYGSRDEMIRAVKHMI
                     ADHDDGKLSADDIDADTFSSYLDTRELPDPDLLIRTSGEQRLSNYLLWQLAYAEFYFT
                     DVPWPAFDKNELKKAIDAYNKRDRRFGGLKETNDTEDK"
BASE COUNT         2934 a         2511 c         2095 g         3261 t
ORIGIN      
        1 tatccatctt ctgtgacagc tcaaccgaat caagctgttt tccatctatc gccagacaaa
       61 atacatatgc atcgtctttt atatttttaa gaagcctctc accttcttta ttttttacga
      121 tgtctatctc tgtctgtgtg gcctgttcgc ttgtcttttc atcggaaacc tcagtgatct
      181 caagcttaca gtaacggctt aggcgctttg agtactcagc aatcgcatct ctgtaaaatt
      241 tttctttaac ttttcctaca cataatattg taattttcat cgtttcatta taacatataa
      301 ctataattaa acaatatgga ggccgtatga aaatatacgt tgatgaacaa aaacgatacg
      361 aggatacaaa agcaaccgga cagtgcttca ccggagtcgg tgcgataggg cttattgcta
      421 ttgtgctgct tgatacaggg gttataaagc ttgccgcttt agactcggta aacaagctca
      481 tggtttctat cgtaatgggg cttgttttct tgatattttt tatcatagga atgaagtcat
      541 ttatggagct taaggacatt tcaaaaaaaa ttgaccggaa taattccctc gaagaagaaa
      601 tcatggaata cgtgaccgtc actcataggg acgagcttat gactttagca tcatctggtg
      661 aaaaagaaga tgcctgctca ggtgatttat actacaaaag agctgagctt atcacatcag
      721 tcattactga aaaatacagc ctgcttgagg aaagcttcct cgatcatatg gttgaagaaa
      781 tttattctaa aatttatagt gatattgtcg aagaatagtg ctataataat aacaatttat
      841 tttttatgag gtgaacaaaa tgaaagaact gcaagatcga attctcaagg atggccaggt
      901 aattggcaca aatatcttaa aggtcaacaa atttatcaac catcaggtgg atccggtgct
      961 tatggagcac atcggtgagg atttcgcaaa tcactttgcc ggtaagggaa tcacaaaggt
     1021 tgtcacaata gagagctcag gaattgctcc ggcacttatg gcagcagcca agatgaatgt
     1081 accgcttgtt attttaaaga agcagccttc aaagacatta cataatgatt tatatcagac
     1141 acaggttact tcatttacaa cagagaagag ctatgagctt acgctctcac gcgatgttat
     1201 ctcagaggat gacaatattc ttttgattga tgatttcatg gctgacggag aggctgctac
     1261 aggtgccatc agacttcttc gtatggctca cgctacagtt gccggtatag gaatccttat
     1321 tgagaagtca ttccagcctg gcagaagaaa gatcaacgag cagggctatg aggtttactc
     1381 acttgcacgt atcaagtaca tggatgagta tcagatagat tttattgaag aaaattaaaa
     1441 taagatttaa tatttaaaat caatacatat tatacgcaaa atccactacc ctatttacag
     1501 atttatgagt ctgtcaatga gtagtggatt tttattattg ttaaagtata tgttttatgt
     1561 taaactgccg gattatgctc cgaaatcgaa aagtgaaagc tggtttgact ccggcagtga
     1621 accaagcagt cccaaatctg acatgaggtc tacggctgtt cgcgatacat gagttctctc
     1681 cctgaaatca tcctttgaga ggaatggtcc gtcctttgcc gcttccgaaa tataaatagc
     1741 ggcattgtcg cctagtccgt caatactgtt tatggccggc atgagcttac cgtcaattat
     1801 ctggaaacgg ttagggtgag ccctgtatat atcaagaggc atgaaatcaa agcctctggc
     1861 atacatctcc tgtacgatac gcatgtcctt aaatacatcc tgctccttgt ttgacagcgt
     1921 gtcttttctt ttcttatagt ccttcatgta gttttcgagt cggtcctttc cctgacacat
     1981 aagctcatat gaaaagcctg tggcacggat tgaaaagtat gctgcgtagt atgcaagagg
     2041 atagaaaacc ttacagtagg cgattctcca tgccatcata acgtatgccg cagcatgggc
     2101 cttagggaac atgtacttga ttttctcgca tgaccagata taccagtcag gcacaccgtg
     2161 tgccttcata tcctccttcc actgtggcca ctgagggcat ttgcccttgg caataatgcc
     2221 ctttcgcact ttctccatga tgttgaaggc ttcctcagat tcaagcccca tggaaatcag
     2281 atatgtcata atatcatctc tggtacagat acaggttgaa atggtagctg tgccttcctc
     2341 gataagagtc tgtgcattgc caagccatac gtcggtaccg tgtgataatc cggcgatacg
     2401 aatgaggtca gaaaactcct ttggctttgt atcaaggagc atgcccatgg caaaatcggt
     2461 accaaactcc ggtataccta aagctccaag cggacatcca tcgatatcct caggctctat
     2521 gcccaaagcg gaggtgttct gaaacagtga catgacagcc ttgtcgtcaa gcggaatctt
     2581 tgttgggtca acacctgtca gatcctgcag catacgaatc atggtaggat catcgtgtcc
     2641 aagtatatcg agctttaaca ggttgtggtc gattgaatgg tagtcaaaat gcgttgtcgt
     2701 gatgtcaacg gtcatatcat tcgcaggatg ctgtacagga gtaaaggtat taatctcctc
     2761 acccatcggc agcacgacta taccacccgg atgctgtccc gtagtacgtc gaacaccaac
     2821 gcagccctgc actatcctgt ctatctcgca gtttcgcttt ctgacaccgc gctcctcata
     2881 atagttttta atgtatccaa atgcggtctt gtcagcaagt gtacctatgg ttccggcttt
     2941 aaaggtctgg ccctctccga aaataacctc ggtatannnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naacggaata tcaaagccct ccttgcttaa
     3121 aggtctgccg cactttggac agagcttatc cggcatatca cagccaccac ggcctgaaaa
     3181 tgcctgtacc tctggtgaat caaaatcact gtatttgcag tgtgtacaca aatagtgagc
     3241 atgcagcgga ttaacctctg tgatacctga catggttgcg acaaatgagc ttcctacgga
     3301 gcctcgcgag ccaaccaggt agccgtcctc atttgatttc cacacaagct tctgtgcgat
     3361 gatgtacatt acggcatagc cgtttgaaat aatagagtta agctctctgt caagacgctc
     3421 cttaactatc ggcggcagcg gatctccgta catcctgttt gccttgttat agcagatttc
     3481 acgaagcatg ccgtcagagt tttcgataac cggcggacat ttatcaggtc tgactggtga
     3541 gatacgctca cacatgtctg ctatcttatt ggtattggtg atgactacct cctcagcctt
     3601 tttgcttccg agatattcaa actcctttag catttcctct gtggtcctta aataaagcgg
     3661 agcctgctca tctgcatcct taaagccctt accggccatg ataatacgac ggtatatctc
     3721 gtcctcggga tttaagaaat gtacgtcaca ggttgcaaca accagctttc caaattcctc
     3781 tccaagcttt acgattcgct tatttatatc gataaggtca tcgtttgaag caatatcgga
     3841 atcctcgtca cgtatcataa atgcgttgtt gccaagaggc tgtatctcca ggtaatcata
     3901 gaaattcaca agtcttgtga tttcctcctg tggtcttccg tttaaaatgg ccctgtaaag
     3961 ctctcctgcc tcacaggctg agcctattaa aagcccatca cgatacttgt taaactctga
     4021 ttttggtatt cgcggtctct tattataata tttgatgtgt gacagcgaga taagcctgta
     4081 gagattggta cgcccctggt cacaggttgc aagaatgatg gcatggtatg taggcatctt
     4141 ccttatcatc tcaacagatg attttgccag ggcatttacc tcatctaagc ttgatatgcc
     4201 cctgtccttt agcttttcta tgaatttgac aaatatctct gcagtacatg cggcatcatc
     4261 caccgctctg tgatgatgat caagcgagat gtttagagcc ttggctactg tatccagctt
     4321 aaatctgttt agctgtggca gaagcactct cgcaagagcc accgtgtcaa caattgtagg
     4381 attatacccc atgccaagca atgcgcagtt tcgctttata aagctcatat caaagtcagc
     4441 gttgtgggcg accatgatgc agtccttgca aaactccata aactgtggca gtatgacatc
     4501 cactgttggc gatgtgataa ccatgtcatc ccttatcgag gtgagctcct caatcctgaa
     4561 cggaataggt acctcagggt ttataaatgt ggaatagcgg tctacaatct cgccaccgat
     4621 gaccttcacc gcacctattt ctatgatttt attcttttcc ggagaaaagc ctgttgtctc
     4681 tatatcaaat accacgtact tgtcatccag cgactgaccg gctgcattct ccacaatatc
     4741 ctttgtatca tcgaccagat atgcttccac accgtaaatg accttgaatt tgtcatcaac
     4801 agtatggtta gcgtcaggga atgcctgcac atcaccgtgg tccgtaatag caatggcctt
     4861 gtgtccccag cgcatggcac acttcacaat atcctttacg tcagacacgc catccatgtc
     4921 actcatcttg gtgtgacagt gaagctcaac acgtttttcg gcagcagtat cctccctctt
     4981 tgatgtaaaa tcaggtatct ttttaagtcc gactattgag cttattgaca aatcactgtc
     5041 atatttatct acagtcacaa ctccctttgc cttaacaaag ttgccagggg caatatatgc
     5101 aagaatatct gctacgtcat catttctggc aaaaagcttg agcatgatgg tgtctgtgaa
     5161 atcagtgacc gcaaacataa tgatggtctt ttcgtttctg atttcacggg attcaaccgt
     5221 aagaatcttg ccctttattg taacctcacc gataggaccg tcaatctttt caatggacat
     5281 ctcttcatca tcaaagcttc ttccgtacac gatatcaggg tcattgtcat atttgcgcct
     5341 gaaatctcct ttgcgctcga actttttctc aaacttcttt tcgaacttct tttcaaactt
     5401 cttctcaggc tttttatcag tgcctgtctt cgcttcattc ttcttatttg aggctttagg
     5461 agaatctgat gtgccactct tttttgtatc agatgtattt tcactgcctc ctacgaacat
     5521 tgcaccgtca tcgacagcca ctttagaatc ttcatcatcg tcatcttttg ccatggcaaa
     5581 tttagtgcgg gcgacaatat tttgcacctc ctgctcaatc tgcttgtcag cattttgacg
     5641 gtatttgctg atttttggct tttcgtactg cagattaacg ataaaatcca tcccacagcg
     5701 ctcgcagaac accttctcaa gaaattctat gaccgaattg cctctggtct ttgcgattat
     5761 tgtctcctca agtgtgactg tgacatgtgt atcgtcggta aagctccact ttgaggtgcg
     5821 cagcagattg tacaaaagta cactgtgact gtttatttcg tcaagaatac tgtcaaaata
     5881 gacatcaaaa agagtcttcg gtgtgtactg cgaagacaac tcgtaagact ctataatttt
     5941 tactgtaata tctttacttt taaatatctg gtttttaatg ctttcctcaa gcttccagat
     6001 attttttttg aaaataaggc gttttgcttt tacatatatt ttgtaaaaat ctcttgcatg
     6061 gtttacggaa attctggtga catcagcatt ctccagcaga ttctccatat ccttgtccag
     6121 cttaagtgta gggaaaactt ccttaaatgt ttttgtcaaa attattcacc ccaatggtca
     6181 agctcatatt ttaatgcacc caacagctcg ctctcaggca cctttttaaa tacctcaccg
     6241 tgctttatga taagaccctc gccgattcct ccggctacgc caagatctgc ttcctttgct
     6301 tcacccggtc cgtttaccac acagcccatg acagcaacct ttatgtcaag gtcatatcct
     6361 gaaaccattg tctccacctg atttgcaagc ccgataagat ctatctgtgt gcggccgcag
     6421 gtaggacatg aaacaacctc tatgccaccc tttctaagac ccagagttct taaaatgagt
     6481 ttggcagact ttatttcctc gagaggactt cctgtgagag acacacgtat tgtatcacct
     6541 atgccctggt ttagtatcag tccaagtccg atagcagact ttatattgcc gcttattatt
     6601 gtgcctgcct ctgtgatacc cacatggagc ggatgagtag tcttatctga aataagctca
     6661 tgtgctttaa cacacatcat gacatctgat gatttgatgc ttatgacaag attgtcatan
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcata ccccatatcc tctatgagat
     6781 ttaccttgtc cagagcactc tccacaagcc cctctgctgt cacaccatga tatttctcta
     6841 ccaggtcttt ttcaagagag ccactgttta caccgacgcg gataggaata tttctctcct
     6901 ttgctacatc cacgacagcc tttacccttt cagcgcttcc tatgttgccg ggattgattc
     6961 ggattttgtc ggctccgttt tcaatcgcag ctattgcaag cttgtaatca aagtgtatat
     7021 ccgcaacaag cggtatgctg atttgtttct taatctcctt aagtgccctt gcggcctcca
     7081 tcgtaggcac agcacaccgg attatatcac agcctaggtc tgtcagtgcg tttatctgcg
     7141 caactgtgtc agctacattc tcagtctttg tatttgtcat tgactgtatg agaatcggat
     7201 tacctccacc gattacttta tttccgattt ttactatttt tgtctccatg tgtatatctc
     7261 caatctgtta taaaatcaga ctattagttt tctgatatca ttgaacatga caacaaccat
     7321 cagaagaagt aaaagcacta tgccaacgag atgcacatag ccttccttct cagggtctac
     7381 tctttttctt cttatggctt caactatcaa aaatacaagt ctgccaccat caagtgccgg
     7441 cagtggtagc aggttcatca caccgaggtt tgcagaaatc agcacacccc agtttagcat
     7501 atttaatatg ccaagccata ctccggacat agaaactgcc tgctcatagg tgtctcccat
     7561 gttttttaca atgccgacag gtccgcttaa gttatttaca gaggcagccc cggtacagag
     7621 cattttaagc ccctctattg tcgtatagat gttgtatttc acctcgtagc agctataaag
     7681 cagtgccttt cccaagccgg gctttgtacg ctctcccgac acgttaaagc cgtacagata
     7741 cctctttgtt gtctcgtcat atttaggctt caatgtggtg gtgtgctttt tgccatccct
     7801 aaggtaggtc acatcaactg tttcaccttc gtgaagcatc gagtagaggc ttatctcgcg
     7861 gtaaaagtgt atattcgtat tgttcagctt tacaatctca tcaccggcct tgataccggc
     7921 ctcctcagcc gcatacccct caataacacc ggcaagcttt ggactgtcat agcctacaga
     7981 gcagatgata atgagagcaa aaagaaatgc catgataaaa ttaaaaaacg ggcccgcgaa
     8041 tacaacgctt atgcgtgccc acacggattt gttgttaaat gccctgtcat cacctgagct
     8101 ttcatcctcg ccctccatga tacacgctcc gccaaacgga agcagcttta tggagtattt
     8161 ggtctctcca cgctgcactc caaaaagagt cggtccaagt cccagacaaa attcattgac
     8221 ctttatgcca tttgctttag caagccagaa atgtccgagc tcgtgaaaaa ttactatgat
     8281 gctgaataat atgattgcaa taataatact aataatgttc aaaattacca ccttttactg
     8341 atatagtcat atgcgcactg ctctgtatca agtatctcat ccactgaagg attttcaata
     8401 aatgatgcat tctccataca tgcctcgatt atatcgatta tttccagata tttaatcttt
     8461 ttattcaaaa acagcgccac tgctctctcg tttgcagcat tgaaggccgt aggaatatta
     8521 cctcctgccc ttgcagcctt catggcgaga gcaagccctt taaaggtatc aagatccggt
     8581 gcctcaaagg tcagatcctt aagcttaaag agatcaagcc tgtcaccgga aagcgcaggt
     8641 ctgcttggat aatacagtgc atactgtata ggtatacgca tatcaggagt accaagctgt
     8701 gctataactg ctccatccgc atactcaaca gccgagtgaa tgacactctg aggatgcact
     8761 acaacctgaa tctgcgacaa atcgacgtca aaaagccatt ttgcctcaat tacctcaagc
     8821 cccttattga ccatggttga tgaatcaata gtgatttttt gacccattga ccagttagga
     8881 tgctttagcg cgtcctccac ccttatacct tcaagctcag agagctttct gcctctgaac
     8941 ggacctccgg atgctgtcag aagaattttt ttaatcggat tcatagaatt tccctgcagt
     9001 gactgaaaga tggcactgtg ctcactatcc accggaagaa tggacacacc atattccttt
     9061 gcaagcggta ttatgatatg gcccgctgta acgagagtct ccttatttgc aagtgcgata
     9121 gtcttttttg ccttgatggc ggcaattgta ggacggatgc caagcatacc gacaattgcc
     9181 gttacaacta tatcgcaagg ctcaatggtg gcacatctaa tgagaccgtc catgccataa
     9241 cctacttcga tatcagtatc cgcaaggctt gtgcgaagct ttttggcatc gttttcatca
     9301 cttacactga caaactgtgg cttaaattcc cttgcctgtt tctctataag ggagagattt
     9361 ctgccacagg aaagtgccac aacctgcata tcaccatgct ctcttatcac atcaagtgtt
     9421 tgtgttccga tagagccggt tgaaccaagt attgatattt ttttcatgat aattcctcga
     9481 tttatatttt tctcagtggt tctgaatagt taccttagct ttttgttttc aatggttctt
     9541 aaatgttact ttttaactta aaaagaagct agccagataa taaatgattg gtgctgtgat
     9601 aatcatgctg tcaaagcggt ctaaaatacc accatgtccg ggtatcagat gtccgtaatc
     9661 cttgatatca taattacgct ttatggctga tgcgcacaaa tctccaacca ttgaaataag
     9721 gcctgcaaca gcagctataa cagcaagtat agcaatctct gtgccgtcta tattcatatg
     9781 atttctgaat ataaagcagt ataatgctgt aagaagcgca ctgccgagta caccacctac
     9841 tgcaccctct atagacttct ttggtgaaag ctttggtgcc attttgtgct ttccaatcag
     9901 cactccgacg cagtaggcac atgtatcaca gccccaagag cataaaaata ccaagaaggc
     9961 aaggtaaagt ccgttgttga gtgtacgtat ctggtagaca tatgaaagca ttgttcctac
    10021 ataaaacata ccaaaaaagg ctgccatgag ctgctttgca tcatatttcg ggtatgacag
    10081 cacatacacg aacatcagta caataagaaa gcccatgaaa agcatcatta catcaggtat
    10141 gaagctccac cttaagttga tgtaaaataa aatcaaagca atatacggta caaccgataa
    10201 gagcgatttt tcaaatttaa acaccctgaa aagctcaaac atacctatac acgaaatcag
    10261 aagtgttgaa aaaagaagca cctcgtttcc tgttactata agaaatatag cggcaattac
    10321 aagaactata ccactaagca gtcttgtctt aaacattatt tatcctccgt gtcgttcgtt
    10381 tcttttaatc caccaaagcg acggtctctt ttattatatg catctatagc ctttttgagt
    10441 tcatttttat caaatgcagg ccacggaaca tctgtaaaat aaaactcagc gtatgcaagc
    10501 tgccagagca ggtagtttga caggcgctgc tcaccacttg tgcggatgag taaatcagga
    10561 tccggaagct ctcttgtgtc aagatatgat gagaaggtat ctgcatctat atcgtcagct
    10621 gaaagctttc catcatcatg gtcagctatc atatgcttta cagcacgtat catctcatct
    10681 ctacttccgt aattaattgc aattgtcagg ttaagacctg tatttactga tgaaacttct
    10741 tcaaggtttc ttattttctc ctgaaaattc tctgaaagcc tgcttatctc accaattacg
    10801 cgcacacgca tattgttttt attggcagtc tttatgcagt ttttaagata gctttcaaga
    10861 agcttcatta gtgcagcgac ttcagactca gggcggttcc agttttcagt ggaaaaggcg
    10921 taaagtgtta gatatttaac gccgaggtcg tt
//
LOCUS       DPUF01000154            2549 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_17949, whole
            genome shotgun sequence.
ACCESSION   DPUF01000154 DPUF01000000
VERSION     DPUF01000154.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_17949"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="DHV81_05475"
     CDS             complement(<1..961)
                     /locus_tag="DHV81_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCI93931.1"
                     /translation="MKWKKYTIETTTAAEDFMSSMLMELGIEGIEIEDNIPLTKEDQA
                     DMFIDFLPELPPDEGISHVSFYIEDDGSDQSDMLRKVKLGLEDLRDTVDVGSGVISSS
                     ETEDLDWINNWKKYFSSFTIGDILIKPTWEEVKPEDADKFMIEIDPGISFGTGKHETT
                     QLCIKQLIKYIEGAKEAPTVLDVGCGSGILSIVALKLGAKEVVGTDLDADCMISTRDN
                     MQVNHLDEKLGTFYVGNLIDDTELQKKVGTEKYDIVVANILADVIIPMAPVIPDRLKE
                     GGYFITSGIIDFKENEVKEAIEAAGLKVIEINHQGEWVNITAQK"
     gene            complement(1197..2366)
                     /gene="dnaJ"
                     /locus_tag="DHV81_05480"
     CDS             complement(1197..2366)
                     /gene="dnaJ"
                     /locus_tag="DHV81_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCI93932.1"
                     /translation="MAEKRDYYEVLGVSKTATDAEIKKAFRQQAKKYHPDIHPGDKEC
                     EEKFKEAQEAYAVLSDPDKRRQYDQFGHAAFDGTGGGAGGFDFSGMDMGDIFGDIFGD
                     FFGGGRSSGRRNGPAQGANVRLSVRISFEEAIFGCTKELEFNYKETCSTCGGNGAKPG
                     TSPETCTQCNGTGKVVRQSQSLFGVVQNVTTCPSCGGSGKVVKDKCPTCHGTGYNTKK
                     VRKTVEIPAGIDNGQCVRIREYGEPGINGGPRGDLLVEVIVSGSRDFERQDVNIFSKA
                     SISYAIAALGGDIRIKTVDGDIIYTVAPGTQTGTRIRLKGKGVPSTRNKAIRGDHYVT
                     LVVNTPTGLSKDAKEALKKFDELSGGSLTKEGGASTDSKKKKGFMDKLKESLDDL"
BASE COUNT          698 a          670 c          417 g          764 t
ORIGIN      
        1 gcttctgcgc agtgatattg acccattcac cctgatgatt gatttctatc accttaagcc
       61 ccgctgcttc gatagcctct ttaacttcat tttccttgaa atctatgata cccgatgtga
      121 tgaaatatcc accttccttt aacctgtcag gaataacagg tgccatagga atgatgacat
      181 cagcaaggat atttgcaaca acgatatcat atttttcggt accgaccttc ttttgaagct
      241 ctgtatcatc tatgagattg ccgacataaa acgtgccaag cttctcatcc agatgattga
      301 cctgcatatt gtcacgcgta gaaatcatgc agtcagcatc aaggtcagta cctacaacct
      361 cctttgcacc aagctttaaa gctacaattg aaaggattcc gcttccacag cccacatcaa
      421 gcactgtagg tgcttccttt gcgccctcaa tatacttgat aagctgcttg atacaaagct
      481 gtgttgtctc gtgttttcct gtaccaaagg agattcccgg atcaatttct atcataaatt
      541 tatccgcatc ctcaggcttt acctcttccc atgtaggctt aatcaatata tcaccgattg
      601 taaatgatga aaaatacttc ttccagttat taatccagtc gagatcctct gtctcagatg
      661 acgaaatcac accacttccc acatcaacag tatctcttaa atcctcaagt ccaagcttta
      721 ccttcctaag catatctgac tgatcactgc catcatcctc aatataaaag ctgacatggc
      781 ttatgccctc atccggaggc agctccggca gaaaatcaat aaacatatcc gcctggtcct
      841 ctttggtcag gggaatattg tcctcaatct cgattccctc gataccaagc tccataagca
      901 tgctgctcat gaaatcctca gccgcggttg ttgtctcaat cgtgtatttc ttccacttca
      961 tataaaaatc ctctcttaat aaaatagata catagtataa atacagtttt atttcaaaac
     1021 atttccgtac attttacaag gcagtcatac gactgcctcc acaacttatc tcatataata
     1081 ccataaaaat gtgctgtata aaagccccat ttttttatac gtaaaaatag actaaaaaat
     1141 gccccgccac acatggcggg acatatatta taggacatct attacagatc atctatttac
     1201 agatcgtcca aagactcctt gagcttatcc ataaatcctt ttttcttttt agaatcagta
     1261 gaagctccgc cctcctttgt cagagaaccg cctgaaagct catcaaactt cttaagagcc
     1321 tccttagcat ccttagacag acctgtaggc gtattgacaa caagtgtgac ataatggtca
     1381 cctcttattg ctttatttct tgttgaagga acacccttgc cctttaagcg gatacgtgta
     1441 cctgtctgtg tacccggtgc aactgtgtat ataatatctc catcaacagt cttgattctt
     1501 atatctccgc ctagggctgc aatagcataa gagatggatg cctttgaaaa gatattaaca
     1561 tcctgacgct caaaatccct gctgcctgaa acaataactt caacaagaag atctccacgc
     1621 ggtccgccat ttatgcccgg ctcgccgtat tcacgtattc tgacacactg tccattatct
     1681 ataccggctg gaatctcaac agtctttctg accttcttgg tattgtagcc tgtaccgtga
     1741 catgtaggac acttatcctt tactaccttg cccgaaccac cgcaggatgg acatgttgtg
     1801 acattctgca ctactccaaa cagagactgg ctctgtctga caaccttacc tgtaccatta
     1861 cactgggtac atgtctcagg agatgtgccc ggcttagctc cgttgccgcc acaggtagag
     1921 catgtctcct tatagttgaa ttcaagctcc tttgtgcagc caaatatagc ttcctcaaaa
     1981 gaaattctta cactgagacg gacatttgca ccctgtgcag gaccatttct tctgccactg
     2041 cttcttccac ctccgaagaa atctccgaaa atgtcaccaa aaatatcccc catatccatg
     2101 cctgagaaat caaagccacc agctccgccg cctgtgccat caaatgctgc atgaccgaac
     2161 tgatcatact ggcgtctctt atccggatca gacaatacgg cataggcctc ctgagcctcc
     2221 ttaaacttct cctcacactc tttatcaccc ggatgaatgt caggatgata tttctttgcc
     2281 tgctgtctga aggctttttt aatctctgca tctgtagcgg tcttagatac tcctaatacc
     2341 tcataataat ctcttttttc tgccataatt gaaaattcct tttattatac aactaattcg
     2401 caaggccggc caagcggcca accttgcgag taattttact tacgcagcca ttatgatgct
     2461 ttgattgata acccgaatta caggtcatta atcctcatat cattatggca gcatctgttt
     2521 aattatactt ccttgaaatc tgcgtcgat
//
LOCUS       DPUF01000155            7271 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_9252, whole
            genome shotgun sequence.
ACCESSION   DPUF01000155 DPUF01000000
VERSION     DPUF01000155.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_9252"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(56..2677)
                     /locus_tag="DHV81_05485"
     CDS             complement(56..2677)
                     /locus_tag="DHV81_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93933.1"
                     /translation="MDKYEYNLKLDQIKALSAEEGYMSAAEIADSINWNKIKNVNTLV
                     KIGEIYEKAERYQDARDILLMAYDRSPIGRMIIYRLAEVAIKMGDYDAATEYYDEFVE
                     IAPHDDMKYVLRYAIKKGQGASFDELITILEEYKDEEYTEEWAYELAYLYHKAGKADK
                     CIDACDELILWFGDGPYVERALELKMLYQPLTKAQEEKYRRFKAEKEKPAKIKDEAEV
                     TEIGAMEMVKGGEIVHDDVTIPQITVNQEKFNTVNLQQEIAKGMQQIMEAKGKNEVAD
                     TMDTIKKIVEDIPYLKLEKEQEEYVQQPEETEHIATDEEIDGSLKLNFKELLGEDADG
                     QMSMVMSEKTQLEHQITGQMSIQDVLEEWEKTRHAAEIALKEAEQQKLESAKARALQE
                     AGDIMERLNDVIPKLDAGVTPKELLEEEYLKVPVDIIEQKAALKEPEEEQKPDMQELY
                     ADETVDEVQEPQEAPEDTIDEAQESQEAPKEAIDEVQESQEVPEDAADEVKEPVGEPE
                     DIIMAEESEKDEILMQLTTMMPEITDDMLNVDDDTSDDEASQEKENVSEKRDFDHVTS
                     FIEQEIAKMTAKNPGLEKKLDMAKTRKMPDISLPEDLDSEEDDSKLKETKHIKELTSE
                     QKAIFSYFIPVKGMEDQICKAYNAVLDHFNRKENASTGNLIIQGEQGCGKTMLATSFI
                     KVLQKDGEQLTGKMGKIDAAALNKKDVQQVVRKITGGCLIIERAGDIDRSIAAQLSFL
                     MEHDITGTLYILEDTSKGIKKALSMDEGFASKFTEKISVPIFTNDELVLFAKSYSAEL
                     GYKIDEMAILALHNRISNIERIDQATTLTEVKDIIDEAIDREAHSGLKKAISILTARR
                     YTDDDRIVLKEDHFREK"
     gene            complement(2687..4000)
                     /locus_tag="DHV81_05490"
     CDS             complement(2687..4000)
                     /locus_tag="DHV81_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCI93934.1"
                     /translation="MEETGKAGKKSNIEINADKGAKQNSHPGREEWPHLIRIGVMVFV
                     TFAVSILFFFALYRFEGFAGIWSKLLAAAMPIIMGLVLAYLMNPVMLWLERCFKKLLS
                     KKMKSESKLRKVSRALAITGSVIILVAIISLLIAAIVPSVIASISGLMKTLPNDVAAF
                     INMIKNGNFGDSKIAELASTGLQNATDYIEDYATEKLIPEAQKYVAQITTGVISVVRG
                     LFNFIIGIIVMVYVMSIQETLAGQSKKIIYAVCKPKTGNIIIETIRKTNEIFGGFISG
                     KIIDSLIIGVIAYFGCLILRIPSSVLVAVIIGVTNVIPVFGPFIGAIPSLLIVVIQSP
                     WHALYLLIFIVVLQQVDGNIIGPKILGSSTGLSTFWVMFAILIGGGMFGFLGMLLGVP
                     TFAVIYYIVKRVVNYALRKKKLPEDTRDYTKTTGVDTKNNKLKYE"
     gene            complement(4016..6301)
                     /locus_tag="DHV81_05495"
     CDS             complement(4016..6301)
                     /locus_tag="DHV81_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCI93935.1"
                     /translation="MKLYENGAYLLNGKEVIIDGPEAQAAVKSKTGMDIDKQTAKQET
                     IAYGILKEHNTSGNMDKLQIKFDKLTSHDITFVGIIQTARASGLEKFPIPYVLTNCHN
                     SLCAVGGTINEDDHMFGLTCAKKYGGIYVPPHQAVIHQFAREMLAGGGKMILGSDSHT
                     RYGALGTMAVGEGGPELVKQLLEQTYDIDMPEVVGIYLTGEPIKGVGPQDVALAIIGE
                     VFGNGFVKNKVMEFVGPGVSNLSVDFRIGVDVMTTETTCLSSIWRTDDQVKEFFEIHG
                     RAEDHKELNPGEAAYYDRFIEIDLSQIRPMIAMPFHPSNTYTIDELNANLMDILDDCE
                     KRAEVSFDGKVKLDLKSKVRDGKLYVDQGIIAGCAGGGFENICDAADILNGHSIGADE
                     FTLSVYPASTPIYMELVKNGAVAKLLETGAIVKTAFCGPCFGAGDTPANNAFSIRHST
                     RNFPNREGSKVQNGQISSVALMDARSIAATAANKGFLTSAADIDVNFTKPKYFFDKTI
                     YENRVFDSHGVADPSVEIQFGPNIKDWPAMSALPENMLLKVVSEIHDPVTTTDELIPS
                     GETSSYRSNPLGLAEFALSRKDPEYVGRAKEIQKAQKAIESGECAGKAVPEVAEIMGV
                     VKKKFPEASHENMGFGSTIFAVKPGDGSAREQAASCQKVLGGWANIANEYATKRYRSN
                     LINWGMLPFIIDEGELPFHNLDYIFLPGIKKEIEDAKTEFEAYVVKDGELKPFTLKMG
                     ELTDDEREIILKGCLINYNRK"
     gene            complement(6347..>7271)
                     /locus_tag="DHV81_05500"
     CDS             complement(6347..>7271)
                     /locus_tag="DHV81_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI93936.1"
                     /translation="THSLMYKGYELLKRVIEKKDIHTQKTKEIMRIQEALEAISEGAL
                     DTTLNVNEFHGQQKKVAEAVNHIRQGLMSSVNESLKNEKLKADLITNVSHDIKTPLTS
                     IVNYVDLLKRENLENENAKYYIHVLEEKSQRLKQLTEDLVETSKITSGNVKLNMQKLD
                     LVELLYQTGGEFNERFESRNLTIVTKIPSEQIFIYADGRQLYRSIENLYTNAAKYALE
                     NTRVYVELEKADKKAVFTIKNVSKNELDIVSNGNVDLTERFVRGERSRTTEGSGLGLS
                     IAKNLTHLMGGKFEIKVDGDLFIATITYNII"
BASE COUNT         1767 a         1843 c         1294 g         2367 t
ORIGIN      
        1 aacaggtaac aatccattgc tgtaaaatta cacatatata tacccccata agtatttatt
       61 tttccctgaa atgatcctct ttgagtacaa ttcggtcatc atctgtatat cgcctggctg
      121 tcagaatact tatagccttc ttcagtccgc tgtgagcctc cctgtctata gcctcgtcaa
      181 ttatatcctt gacctctgtc agagtggtag cctggtctat cctctcaata ttgctgattc
      241 tgttatgcaa tgccagaata gccatctcat ctatcttgta cccaagctct gcagagtacg
      301 atttggcaaa caatacgagt tcatcatttg taaaaatagg gaccgatatc ttttccgtga
      361 acttggatgc aaagccctca tccattgaga gagccttctt tatacccttt gatgtatcct
      421 caagtatata gagtgttcct gtaatatcat gctccatcaa aaatgatagc tgggcagcta
      481 tgctgcggtc gatgtcaccg gctctttcta tgatgagaca gcctcctgtt atctttctga
      541 ctacctgctg cacatccttt ttattgagtg cggcagcatc aatcttgccc atctttcctg
      601 taagctgttc accatctttt tgtaatacct ttataaagct tgtagcaagc attgtctttc
      661 cgcaaccctg ctctccctgt ataatgaggt tgcctgtgga tgcgttttct ttcctgttga
      721 aatgatcaag cactgcatta tatgccttgc agatctgatc ctccataccc tttaccggta
      781 tgaaatatga aaatatagct ttctgttcgc ttgtaagctc ttttatatgt tttgtttctt
      841 tcaatttcga gtcatcttct tcgctgtcta aatcctccgg caatgaaata tccggcattt
      901 ttcttgtctt agccatatcc agcttcttct ccagtcccgg attctttgct gtcatcttgg
      961 ctatttcctg ctcgataaat gatgtcacat gatcaaaatc tctcttctca gaaacgtttt
     1021 ctttctcctg tgaagcctca tcatctgatg tatcatcatc gacatttaac atatcatctg
     1081 ttatttcagg catcatggta gtcagctgca tgagtatctc gtctttctca gactcctctg
     1141 ccataattat atcctccggc tctccaaccg gttccttgac ttcatctgca gcatcttcag
     1201 gtacttcctg tgactcctgt acttcgtcta tagcctcttt gggtgcttcc tgcgactcct
     1261 gtgcttcgtc tatggtatct tcaggtgctt cctgcggctc ctgtacttca tccacagtct
     1321 catctgcata aagctcctgc atatccggtt tctgctcttc ttcaggctcc ttcaaggcag
     1381 ctttctgctc tataatatca accggcacct tcaaatactc ctcctcaaga agttccttcg
     1441 gagtgacacc tgcatccagc tttggaatga catcatttag tctctccatg atgtctcctg
     1501 cctcctgaag tgctcttgcc ttagctgact caagtttctg ctgctcagcc tctttaagtg
     1561 caatttcagc cgcatgtctg gtcttttccc attcttcaag cacatcctgt atagacatct
     1621 gtcctgtaat ctgatgctca agctgagtct tttcactcat gaccatagac atctgtccgt
     1681 cagcgtcttc acccaaaagc tccttgaaat tcaacttaag tgaaccatct atctcttcat
     1741 ctgtagcgat atgctctgtc tcttccggct gctgtacata ctcttcctgc tccttttcga
     1801 gcttgagata aggaatatcc tcaactatct ttttgatggt atccatcgta tcagcaactt
     1861 cattcttgcc cttggcctcc atgatctgct gcataccctt ggcaatctcc tgctgtaaat
     1921 ttacagtatt gaacttctcc tgatttacag taatctgcgg aatagttacg tcatcatgca
     1981 ctatctctcc gccctttacc atctccatgg cacctatctc agtgacctca gcctcatcct
     2041 ttatcttagc cggcttttcc ttctctgcct tgaatcgtct gtacttttcc tcctgagcct
     2101 tggtaagtgg ctgataaagc atcttaagct ccagcgcacg ctccacatat ggtccgtctc
     2161 cgaaccacag aatgagctca tcgcaggcat ctatacactt atcggctttt ccggccttgt
     2221 ggtatagata tgcaagctca tatgcccact cctcagtgta ttcttcatcc ttgtactctt
     2281 caagaatagt gataagctca tcaaatgaag ctccctgccc ttttttgatg gcatatctta
     2341 atacatactt catatcatca tgaggcgcta tctcaacaaa ctcatcataa tactctgtag
     2401 cagcatcata atcccccatc ttgatggcga cctcagccag tctgtatata atcatcctgc
     2461 cgattggtga acggtcatag gccattaaaa gtatgtccct tgcatcctgg tatctttcgg
     2521 ccttctcgta gatttcgccg attttaacga gtgtatttac atttttaatt ttgttccaat
     2581 ttatgctatc cgcaatctct gctgcgctca tatagccctc ctcggcgctt agggctttta
     2641 tctgatccag cttgagattg tattcgtatt tatccactta tttcacctac tcgtatttaa
     2701 gcttattatt tttggtatct acgcctgttg tctttgtata atcccttgtg tcctcaggaa
     2761 gcttcttttt cctgagagca taattaacaa ctcttttcac aatatagtat atcacagcaa
     2821 aggttggaac accaagtaac attccaagaa aaccaaacat tcctccgccg attagtatgg
     2881 caaacataac ccagaaggtc gaaagacctg tagatgagcc aagtatctta ggccctatta
     2941 tattgccatc cacctgctgc agcacgacaa taaatatcag aagataaagc gcatgccacg
     3001 gactctgaat tactactatg agtaacgatg gaattgcacc gataaacggt ccgaataccg
     3061 gaatcacatt tgtgacgccg ataatcactg caacgagcac cgatgatgga atccttaaaa
     3121 tcagacatcc gaaatatgca atcacaccta taatcagtga atctataatc ttaccgctta
     3181 tgaagcctcc aaaaatctca tttgtctttc tgatcgtctc aatgatgata ttgcctgtct
     3241 ttggcttgca gacagcatat attatctttt tgctctgtcc tgcgagagtc tcctgaatac
     3301 tcatcacata gaccatcacg attattccga taataaaatt aaataagcct ctgactaccg
     3361 aaatcacacc ggttgtaatc tgtgcaacat acttctgtgc ctcaggaatc agcttttctg
     3421 tggcataatc ctcaatgtag tcggttgcat tctgtaaacc tgtgctcgcg agctcagcaa
     3481 ttttgctgtc cccgaaattg ccgttcttta tcatgttgat gaatgccgca acatcatttg
     3541 gcagtgtctt cataagtccc gatatacttg ctatgacaga aggcacaata gccgcaatga
     3601 ggagcgaaat aattgctaca agaataataa ccgagcctgt gattgcaaga gctcttgaaa
     3661 cctttcgcag cttgctttcg cttttcatct ttttggaaag aagcttttta aagcatcttt
     3721 ccagccacag catgacagga ttcatcaggt atgcaagcac aagtcccatt ataataggca
     3781 ttgctgccgc aagcagcttg ctccatatgc ctgcaaagcc ctcaaatctg tacagtgcaa
     3841 agaaaaacag tatactgact gcaaacgtga caaataccat gacacctatt ctgatcagat
     3901 gtggccattc ctcacgcccc gggtgcgaat tctgcttcgc acccttatct gcatttatct
     3961 caatgtttga ttttttacca gcttttccgg tttcttccat cggattaaac tctccttatt
     4021 tacgattata gttgatgaga catcccttta atattatctc tctttcatca tctgtgagtt
     4081 cacccatctt cagtgtaaat ggctttaatt caccatcctt gacaacatat gcctcaaact
     4141 ccgtctttgc atcctcgatt tccttcttga ttcccggaag gaagatatag tcgaggttgt
     4201 ggaacggaag ctctccctca tcgatgatga atggaagcat tccccagttg ataaggtttg
     4261 aacggtatct ctttgtggca tactcgtttg cgatatttgc ccagcctccg agtaccttct
     4321 ggcagctggc agcctgctct cttgctgagc cgtcacccgg cttaacagca aaaattgtgc
     4381 ttccgaagcc catattctca tggcttgcct cagggaattt tttctttact actcccatga
     4441 tttcagcaac ctcaggaaca gcctttcctg cgcactcacc actctctata gccttctggg
     4501 ccttctggat ttcctttgct ctgcctacat actcaggatc ctttcttgaa agtgcgaact
     4561 ctgcaagtcc aagagggttt gaacgatatg aagatgtctc tcctgaagga atgagctcat
     4621 ctgttgtagt aacaggatca tgaatctctg aaaccacctt aaggagcata ttctccggaa
     4681 gcgcactcat tgccggccag tccttgatgt ttggtccgaa ctggatttca actgaaggat
     4741 ctgccactcc atggctgtca aatacacggt tctcatatat agtcttgtca aagaaatatt
     4801 ttggctttgt aaagttgaca tcaatatccg ctgctgatgt aaggaagccc ttgtttgctg
     4861 ctgttgcagc gattgaacgt gcatccatga gtgccactga gctgatctgt ccgttctgta
     4921 ccttagaacc ctctctgtta gggaagtttc ttgttgaatg acggatagag aatgcgttgt
     4981 tggcaggtgt atctcctgca ccaaagcaag gtccgcagaa agcagtcttt acgattgcac
     5041 ctgtctcaag aagctttgct acagctccgt ttttcacaag ctccatgtat attggagtag
     5101 atgccgggta aacactcagt gtaaactcgt ctgctccgat tgaatgtccg tttaatatgt
     5161 cagccgcatc gcagatattc tcaaatccac cgcctgcaca gcctgcgatg attccctggt
     5221 ctacatacag ttttccgtct cttaccttac tctttaaatc aagcttcacc ttgccgtcaa
     5281 agcttacctc agctctcttc tcacagtcat cgagaatatc catgaggttt gcattgagct
     5341 catcgatagt ataggtattg cttgggtgga atggcatagc tatcattggg cggatctggc
     5401 ttaaatcaat ctcaataaat ctgtcgtagt aagccgcctc acccggatta agctccttgt
     5461 ggtcctcagc cctaccgtga atctcaaaga attccttaac ctggtcatct gttctccaga
     5521 tagaagaaag acatgtagtc tctgttgtca taacatcgac acctatacgg aagtcaacgc
     5581 tcagattaga cacacccggt cctacaaact ccataacttt gttctttaca aatccgtttc
     5641 cgaatacctc tccgataatt gcaagtgcga catcctgtgg tccaacgccc ttgattggct
     5701 ctcctgtcag gtaaatgcct actacctcag gcatatcaat atcatatgtc tgttcaagaa
     5761 gctgcttaac aagctcaggt ccaccctcgc cgactgccat agtaccaagt gcaccataac
     5821 gtgtatgtga atcagaacca agtatcattt ttccgccgcc ggcaagcatc tctcgtgcaa
     5881 actgatggat aactgcctga tgaggtggta catagattcc gccgtacttc tttgcacatg
     5941 tgagaccaaa catgtggtca tcctcattga ttgttccacc aactgcacaa aggctgttgt
     6001 gacagtttgt aagtacatac gggataggga atttctcaag tcctgaagct cgtgctgtct
     6061 ggatgatacc tacaaatgtg atatcatgcg atgtaagctt atcaaactta atctgaagct
     6121 tgtccatatt gccggatgta ttgtgctcct ttaaaatacc gtaagcaatt gtctcctgct
     6181 tagctgtctg tttatcgatg tccatgccgg tcttgctttt gacagctgcc tgagcctctg
     6241 gtccatcaat tattacttcc ttaccgttta aaaggtaagc accattttca tataatttca
     6301 cttattctac ctccgtatgt gtatttcaaa attcttttat ttactattat ataatattat
     6361 aggtaattgt ggcaataaat aaatcaccat ctaccttaat ttcgaatttt cctcccatca
     6421 aatgggtcag attctttgca atcgaaagtc caagtccact tccttcagtg gttctcgacc
     6481 tctctcccct cacaaaacgc tctgtcaggt ccacatttcc gttgcttact atgtcaagct
     6541 cgttcttaga cacattcttt attgtaaaaa ctgccttttt atctgccttt tcaagctcta
     6601 catacactct ggtattttcc agtgcatatt ttgcggcatt agtatacaga ttttcaatag
     6661 agcggtaaag ctgacgccca tccgcataga taaatatctg ttctgatgga atttttgtaa
     6721 ctattgtgag atttctcgac tcaaagcgct cattgaattc acctccggtc tggtataaaa
     6781 gctcaaccag atcaagcttt tgcatattga gcttcacatt tcccgaggta atctttgatg
     6841 tctctaccaa atcctctgta agctgcttta aacgctgtga cttttcctcc agcacatgta
     6901 tatagtattt ggcattttca ttttccagat tttccctctt aagcaggtca acatagttga
     6961 ctattgaagt cagaggtgtc tttatatcgt gtgatacgtt tgttattaag tctgccttta
     7021 gtttttcatt tttaaggctc tcatttaccg agctcataag accctggcgt atatggttga
     7081 cagcctccgc cacctttttc tgctgcccat gaaactcatt gacattaagt gtagtgtcaa
     7141 gcgctccttc cgagatagct tcaagagcct cctgtatgcg cattatctct ttggtttttt
     7201 gtgtatggat atcctttttc tcaataactc ttttcaaaag ctcatagccc ttgtacatca
     7261 ggctgtgtgt g
//
LOCUS       DPUF01000156            2939 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_21788, whole
            genome shotgun sequence.
ACCESSION   DPUF01000156 DPUF01000000
VERSION     DPUF01000156.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_21788"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="DHV81_05505"
     CDS             complement(<1..1070)
                     /locus_tag="DHV81_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93937.1"
                     /translation="MQIENLINKSTDTKNTDTKTINDMTAGMRVSDYIKTDSSDNMSK
                     KAAVVGSSNSVDMSDTIYARPQAKNEAGNNDGTDMAENLLNDMNQTSENRRNDMIVTA
                     STTTSDDYKAAKDDGYDVIVTETDKIKAVLAKAGVDISIYGDDLSREQLTDITGSQTE
                     AAMLVNQMKAYDIPATDDNIKAGTDAIDRAKSITNISNETKAYLVRNNMNPTVSNVYK
                     ATYSSSQVQDYRQKTKMTEISDELFEELKPQLKQILEEAHIDGSDENLNQCRWLIDED
                     IAVTPDNVLLANQIDGITAAGENVSSDFYARAVTEALAMGKKATDATMSQDGLTFDMA
                     KKACDVLGEATAEDIVTLESDN"
     gene            complement(1335..>2939)
                     /locus_tag="DHV81_05510"
     CDS             complement(1335..>2939)
                     /locus_tag="DHV81_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCI93938.1"
                     /translation="ASIITDIEHFTWTDKEWMDKRAAKSDADVYSSPMAIYECHPGSW
                     MRHPGRDDEGFYTYRELAKSLISYVKTMGYTHIELMGISEYPFDGSWGYQVTGYYAPT
                     SRYGKPEDFAYFVDQCHKNNIGVILDWVPAHFPKDAHGLANFDGTATYEYADPRKGEH
                     PDWGTKIFDYGKNEVKNFLIGSALMWIENYHVDGLRVDAVASMLYLDYGKSDGQWVPN
                     KFGGNKNLEAVEFFKHINTLILGRNHGTVMIAEESTAWPKVTGKVEEDGLNFSYKWNM
                     GWMHDFLDYMKLDPYFRKNNHNKMTFAMSYNESEKYILVLSHDEVVHLKCSMLNKMPG
                     LEGDKFKNLMAGYAFMMGHCGKKLLFMGQEFAQKQEWSEERELDWYLLENPEHKKIQN
                     WVKELLHLYKRNRCLYELDSSWEGFEWINANDADRSIFSFIRKSKDGKNNMLFVINFT
                     PVERPDYRVGVPKHKTYQLVLDSEDPKFTGKTVQEPVKKAAKGRGKAKQSYKKLDYKA
                     VKSECDGRPFSFAYPLAPYGVAVFKY"
BASE COUNT          680 a          739 c          523 g          997 t
ORIGIN      
        1 atgttatctg actctaatgt gacaatgtcc tctgcggtcg cctcacctaa tacatcacag
       61 gctttctttg ccatgtcaaa tgtcagacca tcttgtgaca tagtggcatc tgtagccttt
      121 tttcccatgg ccagtgcttc tgtgactgcc cttgcataaa aatcactgct cacattttct
      181 cctgctgcag tgatgccgtc tatctgattg gcaagcagca cattgtcggg tgtaacggct
      241 atatcttcat ctatcagcca tctgcactga ttcagattct catcactacc gtctatatga
      301 gcttcctcta atatctgttt aagctgtggc ttaagctcct caaacagctc atccgatatc
      361 tccgtcattt tggttttctg cctgtaatct tgtacctgac tgctgctgta cgtagccttg
      421 tatacattgg aaaccgtcgg attcatgtta tttctgacca gatatgcctt tgtctcattg
      481 gatatatttg taatgctctt tgcccggtct atggcatctg tacctgcctt tatattgtca
      541 tctgtagcag gaatatcata tgctttcatc tgattaacaa gcatggccgc ttcagtctgg
      601 cttcccgtga tatctgtaag ctgctccctg ctcagatcat ctccatatat ggatatatct
      661 acaccggcct ttgccagcac tgcttttatc ttatcggtct cggtgacaat gacatcgtag
      721 ccgtcatcct ttgcagcttt gtaatcatca ctcgttgtgg tgcttgcagt gacaatcata
      781 tcatttcgcc tgttttctga ggtctgattc atgtcattta gcagattttc tgccatatct
      841 gtgccatcgt tgttcccggc ttcgttttta gcctgtggtc ttgcatatat cgtatccgac
      901 atatcaacag aatttgacga accgacaaca gccgcctttt ttgacatatt gtcactgctg
      961 tctgtcttta tatagtctga aacacgcata cccgctgtca tatcatttat ggtttttgtg
     1021 tccgtatttt tcgtatctgt gcttttatta atcaaattct ctatctgcat atttcctgct
     1081 ccgtgccctg catatatacc atctctgcag cctttattta tggatattca tacctcgtat
     1141 tttttatcca actgttcaga accaccttga gaacaagaaa atctgctgct tacatgcaag
     1201 catgacgcat cagttttttt gttctcagtg gtcctgaata gttatctttt tattatatat
     1261 cgacatataa tacgcaaaaa taaagaccgc accttaaatt ggcgcggtcc tttaccctgc
     1321 tatttaccgg tatattagta tttaaataca gcaactccgt aaggcgcaag tggataagca
     1381 aatgaaaacg gtctgccatc acactctgat ttaactgcct tataatccag cttcttgtat
     1441 gactgttttg ctttacctct gccttttgca gccttcttta caggctcctg taccgttttc
     1501 ccggtaaact taggatcctc actatctaaa acaagctgat atgtcttgtg ctttggcact
     1561 ccgactctgt aatcaggtct ctctaccggt gtgaaattga tgacaaagag catattattc
     1621 ttgccatcct tgctctttct gataaagctg aagatgcttc tgtctgcatc gtttgcattt
     1681 atccattcaa agccttccca gcttgaatcc agctcataca gacagcggtt tctcttatag
     1741 aggtgaagca gctcctttac ccagttctgg atctttttat gctccggatt ctcaagcaga
     1801 taccagtcga gttctctttc ctcactccac tcctgcttct gtgcaaactc ctgtcccatg
     1861 aaaagaagct tctttccaca gtgtcccatc ataaatgcat atcctgccat gaggttcttg
     1921 aatttatccc cttctaagcc cggcattttg ttgagcatag aacacttcag atgtaccacc
     1981 tcatcatgtg acagtaccag aatatatttc tcactctcat tatagctcat cgcaaatgtc
     2041 atcttgttgt ggttattttt acggaaatat ggatccagct tcatataatc aaggaagtca
     2101 tgcatccatc ccatgttcca cttgtagctg aagttcagtc cgtcttcctc taccttgcct
     2161 gttacctttg gccatgcggt agactcttcg gctatcatta cagttccgtg atttcttcca
     2221 agtatgagtg tattgatatg cttgaaaaac tctactgcct ctaggttctt attgcctccg
     2281 aacttgtttg gcacccactg tccgtcgctc tttccatagt caagataaag cattgacgca
     2341 actgcatcca cacggagtcc gtctacgtgg taattttcta tccacatcaa tgcacttccg
     2401 ataaggaagt tctttacttc attcttaccg tagtcaaata tctttgtgcc ccagtcggga
     2461 tgctctccct ttcttgggtc tgcatactca tatgtggctg ttccgtcgaa attggcaagt
     2521 ccatgcgcat cctttgggaa atgtgcaggt acccagtcga ggattactcc gatattattt
     2581 ttatggcact gatcaacaaa gtatgcaaaa tcctcaggct ttccatacct cgatgtaggt
     2641 gcatagtagc ctgtcacctg atatccccat gagccatcaa acggatactc tgaaataccc
     2701 ataagctcaa tatgtgtata tcccatagtt ttcacatagg atatgagtga ttttgcaagc
     2761 tccctgtatg tataaaagcc ctcatcatcc ctgcccggat gtctcatcca tgagcccgga
     2821 tggcactcat atatagccat aggtgaagaa tatacatcag catctgactt tgctgctctc
     2881 ttatccatcc actctttgtc agtccatgtg aagtgctcga tatctgtgat gattgacgc
//
LOCUS       DPUF01000157            3076 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_22720, whole
            genome shotgun sequence.
ACCESSION   DPUF01000157 DPUF01000000
VERSION     DPUF01000157.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_22720"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="DHV81_05515"
     CDS             <1..480
                     /locus_tag="DHV81_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HCI93939.1"
                     /translation="DDIRVMFYREYKIAENIRHILKDKKQIDIIDDEIGYITLHIHTS
                     LNNSKVSDAMEMAAAVRKCATFIEEKIGKHIDVTTMAYNRLMNHIRHMVSRATTGEKL
                     KVDLNQFIEKNYPESFALAGDICKELGKDLNHEFLDNETGYLAIHIEQIKCDEMISE"
     gene            670..2019
                     /locus_tag="DHV81_05520"
     CDS             670..2019
                     /locus_tag="DHV81_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCI93940.1"
                     /translation="MERHVFKRKIYNEILEWKINRSDKYALLVKGARRTGKSTIVEEF
                     AKNEFKSYILIDFAHTSKDIIALFDDTYELDFFFLQLQQLTGVRLYENESVIIFDEVQ
                     LLPKARQAIKYLVADGRYKYIETGSLISIKKNTMDILIPSEEHKISMYPMDFEEFLWA
                     IDDEITAETIKLLLKNKKAAGNAMHRNLMRKFRLYMLIGGMPQAIEAYLEHNNLQIVD
                     ETKREIVELYEEDFTKIDGTGLAGDIYDAIPASLSKNASRYVLSKAREGVRAKQVRDI
                     LPDILSSYTVNIAYHANNPGVGMSLEKDIDRYKLFTSDVGLFITLAFKDKKYTDNIIY
                     NKLLSDKLEANLGYVYENAVAQMLTAKGNNLFYYTMKSETSNHLYEIDFLLSNGTKIN
                     PIEVKSGNYREHKSLDAFCEKFSSRIREKYVVHTKDYKWENGINYLPVYMIPFIDGE"
     gene            2152..>2387
                     /locus_tag="DHV81_05525"
     CDS             2152..>2387
                     /locus_tag="DHV81_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCI93941.1"
                     /translation="MKLGIVGLPNVGKSTLFNSLTKAGALSANYPFATIDPNVGIVSV
                     PDERIVKLGELYHTKKVTPATIEFVDIAGLVKGA"
     assembly_gap    2388..2582
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2583..>3076
                     /locus_tag="DHV81_05530"
     CDS             <2583..>3076
                     /locus_tag="DHV81_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCI93942.1"
                     /translation="RMDKTLAKELELVEAVKAHLEDGKMARTFEVPDDEDAQLWFKGY
                     NLLTAKPTIYAANVSEDDLADDGASNPHVAKVREMAKEEGAEVFVICAQIEQELADLD
                     EDEKKEYLEDLGVESSGLDKLVAASYSLLGLISFLTAGEDECRAWTIKKGTKAPQAAG
                     KIHT"
BASE COUNT         1012 a          453 c          649 g          767 t
ORIGIN      
        1 gatgacatcc gtgtgatgtt ttatagggag tacaagatag ctgagaatat ccgccacata
       61 ctaaaggata aaaaacaaat tgatataatt gatgatgaga tcggttacat tactcttcat
      121 attcatacat ccttaaacaa ttcaaaggta tcggatgcca tggagatggc ggcagcagtt
      181 cgaaaatgcg ccacatttat agaggaaaag attggaaagc acatagatgt cactactatg
      241 gcctacaacc gcctcatgaa ccatatccgc cacatggtat caagagccac gaccggagaa
      301 aaattaaagg ttgatctcaa tcagtttatc gagaaaaatt atccggagtc attcgcactg
      361 gcaggtgata tatgtaagga gcttggaaag gacttaaatc atgagttttt ggacaatgag
      421 acaggctacc ttgccataca tatcgagcag attaagtgtg atgagatgat aagtgaataa
      481 aaaatgtgaa aaccttcctg catattaggt tatgtgtggg aagttttttt cgtgaaaaag
      541 tactgtgatg gaagaaatga atggggattt gcgtaagatt ataggtaaaa cgaatgggga
      601 tttgcgtaaa atatcagttt acacgagtgg ggattcggga tataatatta acaaagaggt
      661 gacttttata tggaaagaca tgtatttaaa cgtaaaattt ataatgaaat acttgaatgg
      721 aaaatcaacc gaagcgacaa atatgccctt cttgtgaaag gtgccaggcg aaccggcaag
      781 tcgacgattg ttgaagaatt tgctaaaaat gaatttaaat catatatcct gattgatttt
      841 gcgcatacaa gcaaggatat tattgcttta tttgatgaca catatgagct tgatttcttc
      901 tttttacagc tacagcagtt aaccggtgtg agattatatg aaaatgaatc cgttataata
      961 tttgatgagg tccagctttt gccaaaagca agacaggcaa tcaagtatct tgttgctgat
     1021 ggccgatata aatatataga aacaggttca ttgatttcga taaaaaagaa tacgatggat
     1081 atattaatac caagtgaaga gcataagata tctatgtatc ctatggattt tgaagagttt
     1141 ttgtgggcaa tagatgacga aatcacagca gagacgatta aactactttt aaaaaataaa
     1201 aaagcagcag gaaatgcgat gcatagaaat ttgatgcgta aatttagatt gtatatgcta
     1261 atcggaggaa tgcctcaggc tattgaagct tatctggaac ataataatct tcagattgtg
     1321 gatgaaacga aaagggagat tgttgagtta tatgaagagg attttacaaa aatagatgga
     1381 accggactgg caggagatat atatgatgca attccggcaa gtcttagtaa aaatgcatca
     1441 agatatgtgt tgtcaaaagc gagagaaggg gtacgtgcaa aacaagtgag agatatatta
     1501 cctgatatac tcagctcata tactgtaaat attgcatatc atgcaaacaa ccccggggtt
     1561 ggaatgtctc tggaaaagga tatagacaga tacaagctat ttacatcaga tgttggactg
     1621 tttattacgc ttgcatttaa ggataaaaaa tatacggata atataatcta taataagctg
     1681 ttgtcagaca agctggaagc aaacctggga tatgtttatg aaaatgcggt agcacagatg
     1741 ttgacagcaa aaggaaacaa tctgttttat tacacaatga aaagtgaaac ttcaaatcat
     1801 ttgtacgaga tagatttcct gctatcaaat ggaactaaaa ttaatcctat tgaagtgaaa
     1861 tccggaaatt acagagagca taaatcattg gatgcatttt gtgagaaatt tagcagccga
     1921 atcagagaaa aatatgttgt tcatacaaaa gactataaat gggagaatgg gataaattat
     1981 ctgcctgtgt atatgatacc atttattgat ggagaataaa ggatttgttt tagctgagta
     2041 tgttaaaatt atgtacaaat ctccgttaat gtggtacact acatagttgt atcaacaaac
     2101 atccgataca caaaatacat atgaaatcaa tttaaaggag atatatatac catgaaactc
     2161 ggaatcgtcg gacttccgaa cgtcggaaag tctacattat tcaactcact cacaaaggca
     2221 ggagcgcttt cagcaaacta tccttttgct acaatcgacc caaacgtagg tattgtttca
     2281 gtgcctgacg agagaatcgt aaagctcgga gagctctatc acacaaagaa ggttacacct
     2341 gcaacaatcg aatttgttga tatcgcaggt cttgtgaagg gagccagnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnagaatgga caagacactt gcaaaggagc ttgagcttgt tgaggcagta aaggctcatc
     2641 ttgaggacgg aaagatggcc agaacctttg aagtgcctga tgatgaggat gcacagcttt
     2701 ggttcaaggg atataatctc cttacagcaa agcctaccat ctacgcagca aacgtatcag
     2761 aggacgacct cgcagatgac ggcgcaagca atccgcatgt tgcaaaggta agagaaatgg
     2821 caaaggaaga gggcgcagag gtatttgtta tctgtgcaca gattgagcag gagcttgcag
     2881 atctcgatga ggacgaaaag aaggagtacc ttgaggattt aggtgttgag tcaagcggtc
     2941 tcgataagct tgtagcagcc agctattcac ttctcggact tatttccttc ctcacagcag
     3001 gagaggatga gtgccgtgca tggactataa agaaaggtac aaaggcacca caggcagccg
     3061 gcaagataca tactga
//
LOCUS       DPUF01000158            3887 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_647, whole
            genome shotgun sequence.
ACCESSION   DPUF01000158 DPUF01000000
VERSION     DPUF01000158.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_647"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(1..1448)
                     /locus_tag="DHV81_05535"
                     /pseudo
     CDS             complement(1..1448)
                     /locus_tag="DHV81_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516788.1"
                     /note="too many ambiguous residues; incomplete; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M24"
     assembly_gap    579..654
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1453..1914)
                     /locus_tag="DHV81_05540"
     CDS             complement(1453..1914)
                     /locus_tag="DHV81_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCI93943.1"
                     /translation="MANNELQIAGSQGELRIRPYEHHAKYYETDQMGIIHHSNYVKWM
                     EEARLDLMDQIGLNYRQMEDMEIISPVLSISVEYHSMVHFDDTVVIYTKLLKYNGIKM
                     ELEYVMKDKETGELRTTAKSSHCFLNKSGKPISLKRMYPELDTKFFEFKEA"
     gene            complement(2009..3887)
                     /locus_tag="DHV81_05545"
                     /pseudo
     CDS             complement(2009..3887)
                     /locus_tag="DHV81_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742400.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
     assembly_gap    3041..3103
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          943 a          969 c          664 g         1172 t
ORIGIN      
        1 acattgagca catagcccac accgtgtccg gtaccgtgat tgtagtccat gccaatctcc
       61 cagaaaggct ctcttgccag aatatcaaag tttagtcccg agcagccctc cttaaacctt
      121 gcatttgcca ggttcatatt tgagcggcac actctcgtaa acatatcttt catctcgtca
      181 gtcacagggc ctagtgcaaa ggtcctcgtg atatctgtgg ttccctccag ataatgtcct
      241 cctgaatcaa ccaaaagaag actctcaggc ttaagctgcc tgtcggactc ctctgttgca
      301 gagtaatgca caatagcacc atgatcagca tatccgcata ttgtggcaaa gctcaaatca
      361 agaaagccct cctgctcagc tctaagcgat gcaagataat ctgaagccga aatctctgtc
      421 atgggtatct tacctatatt tgtcttaagc cagtacatga acttgcacat cgcaactgca
      481 tccttcaaat gcgccttgcg cagattttca agctgtactt cattctttac agccttcatg
      541 acaactgtag gatcctccgc atcaatcacc ttaacaccnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctttt
      661 tcatcaatat gcttaacata ttcataaaaa tcatcgtaag gtcttgtctg tataccattc
      721 ttatcgaggt atgccttaag agtctctgtc acaacctctt cctgcaaaaa ccatatgcag
      781 ctgtcctgtg acaatgccag gtatgaaaga actacaggca catagcttat gtcgcctccc
      841 ctgacattta aaagccatgc tatatcgtaa agtgatgaca tcagatgaag tgttgcgccc
      901 ttttttgcca tctgctctct gactctttta agctttgagc ttatatcctc accggtatat
      961 ttattgtcaa aaatcatgac agcttccttt gacataggtg gtctgtcagt ccatataagg
     1021 tcaatcagat cctcatttac atacatgctg ccgtgctttt cttttgcgat ttcagaaagc
     1081 cttttgcccc atgcggcatt taccactctg ccgtcaaagc ctattacacc gccatccgaa
     1141 agagtatcct ttatatactc atcaactgtc ggtacgccct cctcgccaat cttaaagagc
     1201 tttacagtgg tatccttaag ctgcgcagcc gcctgcacaa aatacctgcc gtctgtccat
     1261 agtccggcct catccatagt gatgactgcg gtacctgctg agcccgtgaa gcctgtaata
     1321 tatttgcgtg ccttaaaatg ctctcctaca tactctgact catgaaaatc agcagtaggt
     1381 accacataca gcgagatgcc cctctttgcc atctcgcttc tcaatgcttt taatctatcc
     1441 tgtatcattt tattatgcct ctttaaattc aaaaaatttt gtatctaatt ccggatacat
     1501 tctctttaat gagattggtt tgccggattt gttcaaaaag caatggctgc tttttgcagt
     1561 ggtgcgaagc tcgcctgtct ctttgtcctt catgacatac tcaagctcca tcttgattcc
     1621 gttatactta agcagctttg tgtagatgac gaccgtatca tcgaaatgta ccatactgtg
     1681 atactctacc gatatcgaca gcaccggact tatgatttcc atatcctcca tctgcctgta
     1741 gttgagtcct atctgatcca tcaaatcaag tcttgcttct tccatccact tgacatagtt
     1801 tgaatggtgg attataccca tctgatctgt ttcataatat tttgcgtggt gctcgtaagg
     1861 acggattcta agttcacctt gtgagccggc aatctgtaac tcattgtttg ccataaaaac
     1921 ttcctccatt catattttgg tcttatttct attatgccac attggacagc taaattcaac
     1981 gacttgaccg taaaatataa accggctgct acctgcgtat catactgtag cagtctctgc
     2041 atatattgcg tgaatttcca atcggaagta ttctgccgca ataccggcat ctgataatat
     2101 actcatcctt gctctttgat agaagctgca taatctttgc ctgtgttttg tcgcgctcgt
     2161 tttcaagcca gtcctcctcc acaattttgc ccatgcggac tgaaaactga tagtacaaat
     2221 caagcttttt gtaataggat tcgtatttca tgagcccctg atactttgaa tgcttatcag
     2281 gcttgtcgag agagatatca gatgtatacg acatgcagta gcgcagccat tgtctgacaa
     2341 gctccctgtc ctttatatct ataggacatg taatcatctt ataaagcaga tatttgttat
     2401 caaaatctgc gataaagtat cgctccttat acgcatatcc atacaaaaac aatatctcat
     2461 cgacattgat tttcctaaac ggtgctgatg gctttgcctc atgccagagc ttaattatcg
     2521 catcaagcgg cgcatccatt gtaaggagca cctgtgggaa gccaagactt actatatcaa
     2581 tatcctgctc cggtatattt agcgtattct tcagataatt cagattcttt tgtcctaaag
     2641 ctgtcacata gcctgtatca tacagaccaa atctgcctgc acggcccgct atctgtttta
     2701 tctcagagat gacaagcgga cgtcttgata ctccatcaaa cttttccacc tccagaaata
     2761 caattcttct gaccggcaga ttgagtccca ttcctatggc atctgtagac acgacaacct
     2821 gtgtcttttt ctcattaaaa agcgtcatct gcctgcgcct tatttccggt ggcagacttc
     2881 cgtatatgac acttggcttt atgccgtttt cctccagacg ccctgctata ttgagtaccg
     2941 acttctttga aaatacgata aaggcatcgc cctctctcac atcctgcgga aataaaaaag
     3001 gcttatcctc cagctttaga gctgtctttc tctcatactc nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaccgggc tcatgcatat
     3121 atgaatctct cctgcaagtg taccaagaat tgccctggtc cacgaatgtc ccctgtcatc
     3181 atctgctacc atctgtgcct catcaatcac tgcgatatca tactccttgt cacagtcaag
     3241 catctccact gtcgatgctg taattctgct gtctgatacc tcaagacact cctggcctgt
     3301 aagcatggtg catggtatgc ctgcatcatt catcttctca tatacctcaa gtgccagaag
     3361 gcgaagcggt cccagataca ctccattttg cgccagctta agcctctcaa gtgcctgata
     3421 tgtcttaccg ctgttagtcg ggcctatatg gagtatgaag cgccttttca tgctctgtgc
     3481 ttttggaaac tccagctccg gccgagccgg aaccagcgtg acaatctctc ttctaagctc
     3541 cttgctgcgg tactcattgt aaagcgcctt catgctcctt gtacatatcg cacctatata
     3601 ctccttgcgc aaaagcttta aaaaatcttc tgtgagatac tctgcttcct tctcatcaaa
     3661 ctgctccata tccaccctga cgagctgcgg aatgatttct ctatttaaaa tatgctttgc
     3721 attcttattg tttgaatagt aggtaatcaa tgcaagcctt tttatttcct gatgcagctt
     3781 gtcgagtgcc acagtctcat tttttgaaaa tcgcgcatat ttaattgatt ttgctttact
     3841 attgagcctg tgaatatagt gtgccagctt tacaggctta cctgtac
//
LOCUS       DPUF01000159            9331 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_9010, whole
            genome shotgun sequence.
ACCESSION   DPUF01000159 DPUF01000000
VERSION     DPUF01000159.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_9010"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="DHV81_05550"
     CDS             complement(<1..956)
                     /locus_tag="DHV81_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCI93944.1"
                     /translation="MAGKNDGKIRCSFCGKTQDQVNRLISGPNGAFICDECIDICAEI
                     LEEEGVDGHEDKSSAKDDINLVTPEELKAFLDDYVIGQDQAKKVLSVAVYNHYKRVLA
                     GDTSDVELQKSNILMLGPTGSGKTLLAQTLARVINVPFAIADATTLTEAGYVGEDVEN
                     ILLKLIQAADYDIERAEHGIIYIDEIDKITKKSENVSITRDVSGEGVQQALLKILEGT
                     EASVPPQGGRKHPQQELIPIDTTNILFICGGAFDGLEKIIDSRMDTSSIGFNSDVKSK
                     TELNVGEAFKHALPQDFVKFGLIPEFIGRVPITVSLEALDRDA"
     gene            complement(1025..1606)
                     /gene="clpP"
                     /locus_tag="DHV81_05555"
     CDS             complement(1025..1606)
                     /gene="clpP"
                     /locus_tag="DHV81_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HCI93945.1"
                     /translation="MSLVPYVIEQTSRGERNYDIYSRLLKDRIIFLGEEVNETTASLV
                     VAQLLFLESEDPNKDIHLYINSPGGMVTAGLAIYDTMQYIKCDVSTICIGLAASMGAF
                     LLAGGAKGKRYALPNAEIMIHQPSGGAKGQATEIQIAAENILKTKKRLNEILAANTGK
                     PYETIAADTERDNYMSAQEAAEYGLIDSVITNR"
     gene            complement(1693..3043)
                     /locus_tag="DHV81_05560"
                     /pseudo
     CDS             complement(1693..3043)
                     /locus_tag="DHV81_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741851.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
     assembly_gap    2068..2153
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3311..3511
                     /locus_tag="DHV81_05565"
     CDS             3311..3511
                     /locus_tag="DHV81_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small acid-soluble spore protein"
                     /protein_id="HCI93946.1"
                     /translation="MAASSSKKASVPEAKEAMSRFKEEVASELGVPLKEGYNGDLTSK
                     QAGSIGGEMVKQMIMKQEQQMK"
     gene            complement(3640..4185)
                     /locus_tag="DHV81_05570"
     CDS             complement(3640..4185)
                     /locus_tag="DHV81_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family protein"
                     /protein_id="HCI93947.1"
                     /translation="MIKVGIFMADGCEEIEGLTVVDIVRRAKLEIETISITEKAEVTS
                     SHQVTFKTDTTKAQADFDSYDAIVLPGGMPGTLNLGADETVVKTIKSFAAEGKLVAAI
                     CAAPSVLGENHILEGKKATCHPGFEEKLLGAQWLEQPVVVDGNVITSRGMGTAIAFAL
                     ELVRYFTDDATVEHIRQGLVY"
     assembly_gap    4219..4315
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4362..4842
                     /locus_tag="DHV81_05575"
                     /pseudo
     CDS             4362..4842
                     /locus_tag="DHV81_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741848.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
     assembly_gap    4502..4530
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4815..5405)
                     /locus_tag="DHV81_05580"
     CDS             complement(4815..5405)
                     /locus_tag="DHV81_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HCI93948.1"
                     /translation="MDRTPKPGELYRHFKNKLYQIVTVATHSETGEKLVIYQALYDDF
                     GIYARPLDMFVSEVDHEKYPDVKQKYRFEKITLKDKPEDMSGKSISLSTNAIAANAPE
                     AVSVQTQACYEPAPDADEGQAPDPRLLEFLDADTFEEKYNILISMRDIITDRLIDDIA
                     VVMDVVVPEGPLQKRYDDLKNTIKTRQYYEFSNRLR"
     gene            complement(5430..5996)
                     /locus_tag="DHV81_05585"
     CDS             complement(5430..5996)
                     /locus_tag="DHV81_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93949.1"
                     /translation="MDVIIWAIILGAVFYINKKSKSAGSKNTQPNKPRPQQKQMQQIW
                     NSMQNTVQSGMNALNQTCQQKSQPQMQQRVQQPQRPRMQQRVQPRNPDIVQRAVKNNA
                     RFADDTTQKELETMHGHSEAQQKMAQEHSRNCQTLHNKAADGAKIIEAETQSMFGSVE
                     DLIAMGYSGNLEFERDFLGEGLDMINNF"
     assembly_gap    6069..6141
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6142..6815)
                     /locus_tag="DHV81_05590"
     CDS             complement(<6142..6815)
                     /locus_tag="DHV81_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93950.1"
                     /translation="MILGLSDTEKKFKTAMDTAGADMTVVNSWIKLYVKTKKNSSGVA
                     KRYYGVKTGLSSLLSDLKELEQQVIGYCELTGTDRKHFGELIKACKAKSGMFDDEFLI
                     SKVDTDFHTTLDSVVKQGERYLSSFDNGIILQSEIENLIHLTNEGLERKKPDLFALSY
                     FYLGHSNKELAELNFTQKTKRVHEIYYEEFWKDILKQLEACVKQAEAINDKYEGTTDR
                     RTARILS"
     gene            complement(7030..8727)
                     /locus_tag="DHV81_05595"
     CDS             complement(7030..8727)
                     /locus_tag="DHV81_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI93951.1"
                     /translation="MKKRLVALTLVAAMALGMTACGSKSEGKISTNDTSANGTSHTAV
                     SAGVDWTSYDELVDSIRTEADLAKRAEMMHQAEDMLMDTWCVIPLYYYNDQYMLKDYV
                     TDVYSTVEGMKYFYNAKNTKNAGKLNIFMASEPDHIDPALNSTVDGGCLAVNSFEGLM
                     RYNAEGKLEPACAESYEVSEDGLTYTFTMRDGLKWSNGDELTAKDFEWSWRRAADPKT
                     AADYSYLCAVFAGYDDTKGLAADDVVASDDGKTLTVKLKAVTPYFLDLCAFPFFFPVN
                     QKSVEGNDDWANDASDKFVTNGAFTLKEWKHDSSMTYVKNPNYWDADNVTVDEMNVML
                     TSDDVSAYTAYQNGDLDFIDTVPTAEIEAAKKTSEFYTVDNLGTYYVGFNINSKLYSE
                     LGLDADQAKVFRKAICLLIDRQYIIDTIAQGGQKIATTFIPEGVTDGNGGEFKNKDYY
                     GTDYDKNFEEAKELLASIGLLDESTGQVNQTVEFSYLVNNSESNVKIGEAIQADLSKV
                     GINLKVEQQEWNVFLNSRKDGQFDFAREGWLMDYNDPINMLEMFTTKSGNNDMQFGR"
     gene            complement(8819..>9331)
                     /locus_tag="DHV81_05600"
     CDS             complement(8819..>9331)
                     /locus_tag="DHV81_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HCI93952.1"
                     /translation="HEFSGGQRQRIGIARALAVDPKFIVCDEPVSALDVSIQAQVVNM
                     FEDLQKERGLSYLFIAHDLLVVQHISDRIAVMYLGHMMELADADELMDNPIHPYTQSL
                     LSAVPIPDPETARHSKRIVLEGDVPSPLRMPSGCPFRSRCKYATEQCAAARPELTDRG
                     NGHMVACFNR"
BASE COUNT         2293 a         2257 c         1684 g         2812 t
ORIGIN      
        1 gcatccctgt cgagtgcctc gagagaaacg gtaataggta cacgtccgat aaactcagga
       61 atcaatccaa actttacaaa atcctgtgga agtgcatgct taaacgcctc tcctacattg
      121 agctctgtct ttgactttac atctgaattg aagcctatag atgatgtatc catacgactg
      181 tcgataattt tttccagacc atcaaatgct ccaccgcaga taaaaagtat attagtagta
      241 tcaataggaa taagctcctg ctgcggatgc tttcttccgc cctgtggcgg tacagatgcc
      301 tctgtgccct caagaatctt taaaagagcc tgctgtacac cctcgcctga cacatctctg
      361 gtgattgaga cattctctga tttctttgta attttatcta tctcatctat ataaatgata
      421 ccatgctctg cacgctcgat atcataatct gccgcctgaa taagctttaa taggatattc
      481 tccacatcct cacctacgta tcctgcctct gtaagtgttg tagcatcagc gatagcaaat
      541 ggaacattta tcactctggc aagtgtctgc gcaagaagtg tctttcctga gcctgtaggt
      601 ccaagcatga gaatattact cttctgaagc tctacatctg atgtatcacc tgccagaact
      661 ctcttatagt gattgtaaac agcaaccgaa agcaccttct ttgcctggtc ctgtcctatt
      721 acataatcgt ccagaaacgc cttaagctcc tccggagtaa caagatttat atcatcctta
      781 gcgctgcttt tgtcctcatg gccatctacg ccttcttcct caagaatctc ggcacatata
      841 tctatacact catcgcagat aaaagctcca ttaggtccgg aaatcaaacg atttacctgg
      901 tcctgtgtct taccgcaaaa tgagcagcga atctttccgt cgttttttcc tgccattatt
      961 aactcctttt attgcatgag ggcatcccat atggggtacc ctcttaaatt catctaactt
     1021 ctgattatct gtttgttatt acactgtcta tgagaccata ctctgcggct tcctgtgctg
     1081 acatatagtt gtctcgctct gtatcagctg caattgtctc gtatggcttg cctgtattgg
     1141 ctgctaaaat ttcgtttaat ctcttctttg tctttaaaat attttctgca gcaatctgaa
     1201 tctcagtagc ctgacccttt gcaccgccag atggctgatg aatcataatc tctgcatttg
     1261 gaagagcata tctcttgccc tttgcaccgc ctgcaagaag gaaagctccc atgcttgctg
     1321 caagaccgat acagattgtt gaaacatcac acttgatgta ctgcatagta tcgtaaatag
     1381 caagtcctgc tgtaaccatt ccacccggtg aattgatgta aaggtggata tccttattcg
     1441 gatcctcgga ctcaaggaat aaaagctggg caacaacaag gctggctgtt gtctcattta
     1501 cttcctctcc aaggaaaatg atacgatctt ttaataatct ggagtaaatg tcgtagttgc
     1561 gctctcctct gctggtctgt tcaatgacat atggtactaa actcatataa aatacctcct
     1621 ttataaaagc tcaaaagacc ggctcagaaa ataggcttct gagccagtca atcaaatctt
     1681 gattcaaaaa tcttattctt ctgtagactc ttctgcatca gcctttttct ttgcctttgc
     1741 acgctcttta acgttgtcca tgataagatc tacggccttt gtgatagcaa tgtctttctt
     1801 catagactcc ttctcagcat ctcccatgta ctctttaagc ttatcagcct ccatgccgta
     1861 tgccttagcc atcttctcta cctcagcatc gatatctgca tcagtaacct cgatgttttc
     1921 ctccttggca atctgctcaa gtacaaggct gctctggata cgtgttgtag cctcaggacg
     1981 aacctgatcc ttaagctggt caactgtcat gcctgagaac tgtaagtact gctccattgt
     2041 aagaccgctc tgtgcgattc tctgagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgataa
     2161 tcttctggat agcctcatcc tctttagtct tcttagcttc tgtctctttc ttctctgtga
     2221 ggttcttttt gatttcctct ttgtactcag ctaatgtatc aacacctgct gcatcctgtg
     2281 caaactcatc atcaaggtca ggaagctcct tagccttgat ctcgttgatc ttaaccttga
     2341 atgtagccgg ctttcctgca aggtctgctg cctgatactt ctcagggaat gttacattga
     2401 cctcaacctc atcgcctgtg ttctttccta ctaactgatc ctcgaatgta tcgatgaatg
     2461 tgtgtgaacc gatctctaat ggatgattct caccctttcc accctcgaaa gctactccgt
     2521 caacaaatcc ctcaaagtcg ataactgctg tatctccctc tgctacagga cgatctgtaa
     2581 ctgtaactgt tcttgcattt ttgtttctct ggttctcaag ctcagcgtca acctcttcat
     2641 cagaaactga tgtgtcgatc tttgtaactg taacgcccat gtacttacca agagtaacct
     2701 caggtcttac agcaacctct gctgtgtaga tgaaaggctg tcccttctca atctgagtaa
     2761 cctcaactgt tgggcgtgaa acaatgtcaa caccgctctc gtcaactgca cctgcgtatg
     2821 tctgctgcat aagaatgttt gctgcatcct cgtagaacac acctgctcca tacattttct
     2881 caatcatagc tcttggcact tttcctttac ggaatccagg tacactaatc tgtttcttct
     2941 gcttctgata tgcagaatcc aatgctttct ctaattcctc agctgctacc tcaatagtaa
     3001 gcttcgccat attatgctct aaattctcaa cctgtacact catgtcaatc gttatcctcc
     3061 ttaaaaatat atatgaatgg tatgtacatc aaatgtaatg tgggcatgct ttttaaactg
     3121 ctaagcccag tcattggatg attataacac aacaaatcca ctatttccag tgttttttac
     3181 attaaatttg cttttgtgta taattccata aattgtcagc aataccaaaa catatttttg
     3241 tcattttacg gcataatatt tccatacaca tacagcttgt gtataacgtt ttaaaaggag
     3301 gtattgcatt atggcagcat caagtagtaa aaaggcttct gtaccggagg caaaagaggc
     3361 tatgagccgt ttcaaggaag aggttgcttc agagcttgga gttcctttaa aagagggcta
     3421 caacggtgac cttacttcta agcaggccgg ttcaatcggt ggcgaaatgg tcaagcagat
     3481 gatcatgaag caggagcagc agatgaaata agctctgcaa tagcaggctt caaatgtacg
     3541 cttcaaatgc aagcataaaa agaggccgcg caccatgggg ttggtgtgtg acctcttttg
     3601 ttataatact aataatatga ttatttgctg aacgttgtat tagtatacca gtccctgtcg
     3661 aatatgctct actgtggcat cgtctgtgaa gtatctgaca agctcgagtg caaacgcaat
     3721 agctgttccc attccacgac ttgtaatcac attgccatcc acaactacag gctgctcaag
     3781 ccattgtgca ccgagcagct tttcctcaaa gcccggatga caggtagctt ttttgccctc
     3841 aagtatatga ttctcaccga gcacgctagg cgctgcacat atcgctgcaa caagttttcc
     3901 ctcagcggca aagctcttaa ttgtctttac tactgtctca tccgcaccga ggtttaatgt
     3961 acccggcatg cctcccggca gtacgattgc atcgtagctg tcaaaatcag cctgtgcctt
     4021 tgtggtatct gtcttaaatg tcacctgatg agagcttgtc acctcggctt tttccgtgat
     4081 tgatatggtt tcaatctcaa gctttgctct tctaactata tctactactg taagtccttc
     4141 aatctcctca cagccgtctg ccataaaaat tcctacttta atcatatttt tctcctttat
     4201 ttttctttga taatactann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntataa
     4321 accgtaacta taaatctacc agtaacctga aaggatatac catggaaatc gaaagaaaat
     4381 acttacttaa acagcttcct tccaatttag acagctacgc gagcaaaaaa atcgcccagg
     4441 gctatctgtg cacctcgcct gttgtcagag tcagacgctc aaatgacagc tattatatga
     4501 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tacaagggag ccggtcttat ggtgcgcgag
     4561 gagtacaatc ttcctctcac aaaagaagcc tacgaccatc ttgtccaaaa gattgacggt
     4621 cgtctcatcg aaaagacacg ctatctcata cctcttgacg gtggtctgac cgcagagctt
     4681 gatgtatttg agggtgatct ggctcctctt acacttgtgg aggttgaatt cgactctgtc
     4741 gacgcagcaa atgaattcac agcgcctgac tggtttggcg aggatgtcac tgaatccggc
     4801 aaatatcaca acagctatct gagccgattg gaaaattcat agtactgcct ggtctttatc
     4861 gtattcttta aatcatcata ccgcttttgc aatggaccct caggaactac aacatccata
     4921 accaccgcta tgtcatctat aaggcggtcg gttatgatat ccctcattga aataagtata
     4981 ttgtacttct cttcaaaggt atccgcatca aggaactcca gcaaacgcgg atccggtgcc
     5041 tggccttcat ctgcatccgg tgccggctca taacatgcct gtgtctgaac actcacagct
     5101 tcaggtgcat tagctgcaat cgcattagtg ctcaaagaga tactctttcc cgacatatcc
     5161 tccggcttat ccttaagtgt gatcttctca aatctgtatt tctgcttcac atcaggatat
     5221 ttctcatggt ccacctcgct tacaaacatg tcgagtggtc tggcgtatat cccaaaatca
     5281 tcatataaag cctgataaat cacaagcttt tcccctgtct ctgaatgtgt cgccactgta
     5341 actatctgat acagcttatt cttaaagtgc ctgtaaagct ctccgggctt tggtgttctg
     5401 tccatcattt ctgcctcttt tcctattatt taaaagttgt taatcatgtc taagccttcc
     5461 ccaaggaaat ctctctcaaa ctccagattg ccgctgtatc ccatggcaat cagatcctca
     5521 acactgccaa acatgctctg tgtctcagcc tctataatct ttgcaccatc agctgcttta
     5581 ttatgcagtg tctggcagtt tcttgagtgc tcctgagcca ttttctgctg tgcctcgcta
     5641 tgtccgtgca tagtctcaag ctctttttgt gtcgtatcat ccgcaaagcg tgcgttattt
     5701 ttcacggccc tctgcactat atccggattg cgtggctgta ctctctgctg catccgtggt
     5761 ctctgaggct gctgtactct ctgctgcatc tgtggctgtg atttctgctg acatgtctgg
     5821 ttcaatgcat tcatacccga ctgaactgta ttctgcatgc tgttccatat ctgctgcatc
     5881 tgcttctgct gcggtctcgg tttgtttggc tgcgtatttt tgcttcctgc gctctttgat
     5941 tttttgttaa tgtaaaaaac agctcctaag ataattgccc aaattataac atccatattc
     6001 tgctccttta tcaatctgca ttttctatgt caatccctga atatcctgca catatcgcgc
     6061 agtatgtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn ngacagtatc cttgccgtgc gccggtctgt cgtgccctca
     6181 tacttatcgt ttatggcctc agcctgcttc acacatgcct caagctgctt taatatatcc
     6241 ttccagaact cctcgtaata tatctcatgc actctcttag tcttttgtgt gaaatttagc
     6301 tctgcaagct ctttgttgct gtgccccaga tagaaatatg acagagcaaa caaatcaggc
     6361 tttttccttt caagcccctc atttgtcagg tgtatcagat tctctatctc actctgtaaa
     6421 atgataccgt tgtcaaagga tgacagatat ctctccccct gctttacaac agagtccaga
     6481 gtcgtgtgaa aatccgtgtc caccttgctg attaaaaatt catcgtcaaa catgccgctt
     6541 ttcgctttgc aagcctttat cagctctcca aagtgcttcc ggtctgttcc tgtgagctca
     6601 caatacccga taacttgttg ctcaagctcc tttaaatctg aaagcagact gcttaagcct
     6661 gtcttcacgc cataatagcg ctttgcaaca ccggaggaat tctttttggt tttgacatac
     6721 aacttaatcc agctgttgac cactgtcatg tcggcacccg ccgtatccat cgctgtctta
     6781 aatttctttt ccgtatcact tagtcctaaa atcatcacac accctcaaac atcattctaa
     6841 aactatataa taacaattct acaccattat catatttttt taaagagcct tttttcgctc
     6901 tgaaaagccc actttaaacg caaaaaaagg aagccataca atagctgtat gacttcctgt
     6961 atactctgca tgttattcgc atcgtcgtgc gaatggcacg agcggctttg ttatgatagc
     7021 aaaatcaaat taacgaccga actgcatatc gttgttacct gattttgttg tgaacatctc
     7081 aagcatgttg attggatcgt tgtaatccat aagccatccc tcacgggcga aatcaaactg
     7141 tccgtcctta cggctgttaa ggaatacgtt ccactcctgc tgctcaacct ttaagttgat
     7201 acctaccttt gaaagatctg cctggatagc ctcaccaatc tttacatttg actctgagtt
     7261 gtttacaagg tatgagaact cgacagtctg gtttacctga cctgtgctct catctaataa
     7321 tccgatagaa gcaagaagct ctttagcctc ctcaaagttc ttatcgtaat ctgtgccgta
     7381 gtaatctttg ttcttgaact cgccaccgtt accatctgta acgccctctg ggataaatgt
     7441 tgtggcaatc ttctgtccgc cctgggcgat tgtatcgata atgtactgac gatcgataag
     7501 aagacagatt gcttttctga ataccttagc ctggtcagca tctaatccaa gttctgagta
     7561 gagcttagag ttaatgttga agcctacgta gtatgttcca aggttgtcaa ctgtgtagaa
     7621 ctcgcttgtc ttcttagctg cctcaatctc ggctgtagga actgtatcga tgaaatcaag
     7681 gtcaccgttc tggtaagctg tgtaagctga aacatcatca gatgtaagca ttacgttcat
     7741 ctcatcaact gttacattgt cagcatccca atagttagga ttcttaacat atgtcatgct
     7801 tgaatcatgc ttccactcct tgagtgtgaa tgcaccgttt gtaacgaact tgtcgcttgc
     7861 atcgtttgcc cagtcatcat ttccctctac tgacttctgg tttacaggga agaagaatgg
     7921 gaatgcacat aagtcaagga agtatggtgt aactgcttta agcttgacag taagagtctt
     7981 tccatcatca gaagctacta catcatcagc agcaagaccc tttgtatcat catatcctgc
     8041 gaatactgcg caaaggtagc tgtaatctgc agctgtctta ggatctgcag ctctcctcca
     8101 agaccactcg aaatcctttg cagtaagctc atcaccgttt gaccacttaa gaccgtcacg
     8161 cattgtgaat gtgtatgtga ggccatcctc agaaacctca tagctctctg cacatgcagg
     8221 ctcaagcttg ccctcagcgt tgtaacgcat aagaccctca aagctgttta ctgcaagaca
     8281 tccgccgtca acagtactgt ttaatgcagg atcgatatga tcaggctctg atgccatgaa
     8341 gatgtttaac tttccggcat tctttgtgtt ctttgcattg tagaagtatt tcataccctc
     8401 tactgttgag tatacgtctg tgacataatc cttgagcata tactggtcat tgtagtagta
     8461 aagtggaata acacaccatg tatccataag catatcctct gcctggtgca tcatctctgc
     8521 acgctttgca agatctgctt ctgtacggat agaatctact aactcgtcat agcttgtcca
     8581 gtctacgcct gctgatacag cagtatgaga agtgccgtta gcagatgtgt cgttagtaga
     8641 tattttgccc tcgctcttgc ttccacaagc agtcatgcca agtgccatag ctgcaacaag
     8701 agtaagtgct actaatctct ttttcatctt tcttcctcct aattaattaa tatgaaaaat
     8761 aaatttatgt tagaaccggc acatgtggct taagctatat gccggtaaaa accttatatt
     8821 agcggttgaa gcatgcaacc atatgtccgt ttcctctgtc tgtaagctcc ggacgtgctg
     8881 ctgcacactg ttctgtcgca tatttacaac gcgaacggaa tggacagccg ctaggcatgc
     8941 gaagaggcga aggaacatct ccctcaagca cgattcgctt gctgtgtctg gcagtctcag
     9001 gatccgggat cggaaccgct gaaagcaacg actgtgtgta cggatggatt ggattgtcca
     9061 tcagctcatc cgcatctgca agctccatca tatgacctaa gtacataaca gctattctat
     9121 cagagatatg ctgcactaca agcagatcgt gcgctataaa caggtatgat aaccctctct
     9181 ccttctgcag atcctcaaac atgttgacta cctgggcctg aattgaaaca tcaagtgccg
     9241 ataccggctc atcacataca ataaacttcg gatctactgc aagcgctctt gctattccga
     9301 tacgctgtct ctgtccaccc gaaaactcat g
//
LOCUS       DPUF01000160            4483 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_32133, whole
            genome shotgun sequence.
ACCESSION   DPUF01000160 DPUF01000000
VERSION     DPUF01000160.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_32133"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="DHV81_05605"
     CDS             <1..408
                     /locus_tag="DHV81_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCI93953.1"
                     /translation="ADFSSVKREAADMKSDNKDALATVRYKYGDHVVGCVDLLKSGAK
                     VDLSYFDENSRQNVESDIIRIKPVYFLWVLVLIAVILLSIFMIRRISDNIYVLKHKWS
                     IKKEQRERFREHKLERKRRRRRDNLKFSGRHKY"
     gene            446..1297
                     /locus_tag="DHV81_05610"
     CDS             446..1297
                     /locus_tag="DHV81_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCI93954.1"
                     /translation="MSDERCALVLEGGAMRGMYTAGVLDCFMDHDISFDAVIGVSAGA
                     LFGVNLLSKQRGRVIRYNKRFNSDKNYMGIRPLLREKNIVSTKYAYDTVPRELDPFDD
                     EEYKKSDIPFYAVVTNIRTGKPEYVQIKSVFDQMDVLMASGSMPVVSKPVKLGNEWYL
                     DGAVTDSIPYEHMLDMGYDRVVVVLTKPADYIKKPMPRLFTSVYKKRFPRFYEAFSNR
                     HIMYNYQLEHLKELEKQGIAKVLRPSEHIKISKIEKDPNKLESLYQLGLRDAKRLLAD
                     KQGMEKQ"
     gene            1297..1902
                     /locus_tag="DHV81_05615"
     CDS             1297..1902
                     /locus_tag="DHV81_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93955.1"
                     /translation="MNIQQVQTIIADAMEKHNGIVKKEELTRLGIDYRRIDKLVREGK
                     LVRIRNGYYTDRVDDFSEDALVAALFPDAMLCMESALYAYGYIKEKPYGWTLAVDKNT
                     SKSRFKMDYPRIIPFYTEPDALMLGASTIELGGNTFGIYDKERVICDCLKYEARMDRN
                     DFKAAIQSYIADPDKDISVLMDYARARRVVKNVQSFIGVWL"
     gene            1893..2825
                     /locus_tag="DHV81_05620"
     CDS             1893..2825
                     /locus_tag="DHV81_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93956.1"
                     /translation="MVVVRVDLRHISDEKKLSYSDVLWQYVKQDFLWRINNAGLGKVL
                     WLQKPYFATGEKLAFYYVYGSPGGRAGDNGTPFDVLRNIIIGNAGLNSHSDVVWTNAE
                     LPKQTAKVSANEPDASDMQPLIHTWNLSAMCDGGEVPFAVRLERLNEADAGVPMTKNI
                     VFMNSFYGDADVCIFSPESRLGEQLFEIMDKLELVSDMKPYGDAYEILKRYSVSGRQI
                     IDNFKVRAKNKPKTVSMHRLEQVYGYRSYAYMKRRWEQYCKRLTRQQRAYANAGWEET
                     LDLILDFLTPVWSAFCNDEIFYDDWMPELGRFLG"
     gene            2847..4290
                     /locus_tag="DHV81_05625"
                     /pseudo
     CDS             2847..4290
                     /locus_tag="DHV81_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515850.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     assembly_gap    2938..3026
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4352..>4483
                     /locus_tag="DHV81_05630"
     CDS             4352..>4483
                     /locus_tag="DHV81_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCI93957.1"
                     /translation="MGKIFCVMGKSASGKDTIYNKILQDDSLMLSRIIPYTTRPIRDG
                     "
BASE COUNT         1305 a          768 c         1196 g         1125 t
ORIGIN      
        1 gcggattttt caagcgttaa aagggaagct gctgacatga agtccgacaa caaggatgct
       61 cttgctacgg tcagatacaa gtatggtgat catgtggtag gctgtgtgga tctgctgaaa
      121 tcaggagcaa aggtcgatct gtcgtacttt gatgagaaca gcagacagaa cgtggaaagt
      181 gatataataa ggataaagcc ggtatatttt ctctgggtgc tcgtacttat cgcagtgatt
      241 cttctgagca ttttcatgat tagaagaata tcagacaata tatatgtgtt gaaacacaaa
      301 tggagcatta agaaggagca gcgcgagcgc ttcagggagc acaagcttga gagaaaacgg
      361 cgcagaagga gagacaatct gaagttcagc ggccgacata aatattaata cattttttat
      421 tatgacatta taaatggagg cagaaatgag tgatgaaaga tgcgccctcg tgcttgaggg
      481 tggagccatg agaggtatgt atacagccgg agtactagac tgttttatgg accatgatat
      541 tagctttgat gctgttatcg gtgtttcggc gggagcactt tttggagtga atctgctgtc
      601 aaagcagcgc ggcagggtta tacgatacaa taagaggttt aacagtgaca aaaattatat
      661 gggtatcagg ccacttctta gggagaaaaa tatagtgagc acgaaatatg cctatgatac
      721 cgtaccaaga gagcttgacc cgtttgatga tgaggaatat aagaagtcgg atatcccatt
      781 ttatgcggtt gtgacgaata tcaggacggg taagcctgaa tatgtgcaga taaagagtgt
      841 ctttgaccag atggatgtgc tcatggcatc gggctccatg cctgtagtgt caaagcctgt
      901 gaagctgggg aatgagtggt atctggatgg cgcggtgacg gacagtattc catatgagca
      961 tatgcttgat atgggctatg acagagtggt tgtcgtgctg acaaagccgg cagactatat
     1021 aaaaaaacct atgccaaggt tatttacatc ggtatacaaa aagcgttttc cgaggtttta
     1081 cgaggcattt tcaaacaggc atatcatgta caattatcag cttgagcatc ttaaggagct
     1141 tgaaaagcag ggcattgcta aggtgttaag gccgtcggag cacattaaaa taagcaagat
     1201 agaaaaggat cctaataagc ttgagagcct gtatcagctt ggattacgtg atgccaagag
     1261 gcttttagcc gataagcaag ggatggagaa gcagtaatga atatacaaca ggtacagacg
     1321 atcatagctg atgccatgga aaagcacaac ggaatagtga aaaaagaaga gcttacaagg
     1381 cttggtatag actacaggcg aattgataag cttgtcaggg aggggaagct tgtccgcatc
     1441 agaaacggct attacaccga ccgcgtggat gatttttctg aggatgcact tgtcgcggca
     1501 ctttttccgg atgcaatgct ttgcatggaa agtgcacttt acgcctatgg ctatataaag
     1561 gagaagccat acggatggac gcttgctgtg gacaaaaata catcaaaatc acgctttaag
     1621 atggactatc caagaattat cccgttttat acggagccgg atgcgcttat gcttggtgct
     1681 tcaaccattg agcttggcgg taatactttt ggcatttatg ataaggagcg tgtgatatgc
     1741 gactgcctga aatatgaggc aagaatggac cgcaatgatt tcaaggcagc catacagtcg
     1801 tacatagctg atccggataa ggatatttcc gtgctcatgg attatgcaag ggcaaggcgc
     1861 gtggtaaaga atgtgcaaag cttcatagga gtatggttgt agtgagggta gatttaagac
     1921 atataagcga tgagaaaaaa ttatcgtaca gcgatgtgtt atggcagtac gtgaagcagg
     1981 attttctctg gcgtataaat aatgccggtc tgggaaaagt gctgtggctt caaaagccgt
     2041 attttgcaac aggagaaaag ctggcgtttt attatgtata tggcagcccc ggtggcaggg
     2101 caggggataa tggtacgccg tttgatgtac tgcgcaatat cattataggc aatgcagggc
     2161 ttaatagtca cagtgatgtg gtatggacta atgcagaatt acctaagcag acggcaaagg
     2221 tatcagctaa tgaaccggat gccagtgata tgcagccgct gatacacacg tggaatctaa
     2281 gcgctatgtg tgatggcggg gaagtaccgt ttgcagtgag gcttgagcgc cttaatgaag
     2341 cggatgcagg agttcccatg actaaaaata ttgtttttat gaacagcttt tatggagacg
     2401 cagatgtttg tattttttcg cctgaatcca ggctcggaga acagctgttt gagataatgg
     2461 ataagcttga gctggttagc gatatgaaac catatggtga tgcatatgag atacttaaga
     2521 gatattcggt cagcggaagg cagataattg ataattttaa ggttagagcg aaaaataaac
     2581 cgaagacagt gagcatgcac aggcttgagc aggtgtatgg ttatcgcagc tatgcgtata
     2641 tgaaaaggcg ctgggagcag tattgtaaga ggctgacacg acagcagagg gcatatgcga
     2701 atgcaggctg ggaggagacg cttgatttga tacttgattt cctcacaccg gtctggagcg
     2761 cgttctgcaa tgatgaaata ttttatgatg actggatgcc ggagcttgga agatttctgg
     2821 gctaaaaagg atatataggt acttaaatgg aaaaagaact gataaataat tcacaatcaa
     2881 atatttatcc gtttcacatg ccggggcata agcgccgtgg cagtgatata ggtgcagnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnntgca tattttctca taaacggaag cacctgcggt
     3061 atactggcag caatatctgc ggccaccaaa aggggagaca aggtgcttgt agcaaggaac
     3121 tgtcacaagg cggtgtatca tgcgctttac ttaaggcagc ttcacccggt gtttacatac
     3181 cctgaaatta cacggacagg gctgcagggg cagataaccg aggcacagat aagggcggca
     3241 tttgatgaaa atcccgacat aaaagcggtt gtgataacct ctcctacata tgatggtgtg
     3301 gtttccgatg tggcggcaat cgcatctgtg gcacatgcac atggagcttt gctcatagtt
     3361 gacgaggcac atggcgcgca ctttggcttt ggaggcggct tccctcaaaa tgccatagcg
     3421 ctcggggccg atgcggtcat agtgagcctg cacaagacgc ttccggcatt tacacagacg
     3481 gcgcttttgc tactgggtgg gatgcgtgaa gctgcagtgg agaagtttct cggcatttat
     3541 gagacaagct caccgtcata tgtgcttatg acaggcatag agcgctgcat acactatgtg
     3601 cgcgataata aagagacagc atttgcacag cttaggagca gacttgacag attttatcag
     3661 aaggtcagcg gactttcgca tctgaaggtg gtcagaaaaa gtgatttttc tgctgacgag
     3721 gcatatgatt ttgacgagtc aaagattatt atatttacga aagaggctat gaatggacat
     3781 acgctgcttg agcttctgct caaaaaatat gagctgcagc ttgagatggc agcaggcaat
     3841 tatgtactgg cgctggcatc tgtcatggat acggatgagg gctttgacag gcttgcggat
     3901 gctttgattg aaatagacag ccagctcgaa aaatataagt ctgattttga agagttttat
     3961 gatatattaa aacaggaggg tgtggtacat aaattttacc tgcctgtcaa aatggataca
     4021 actatatacc aaaagctgcc tgccgtaatg gaaatgtatg atgcatatga tgcagacaat
     4081 caggcggtct atgcctttaa ggcaagcggg aaggtgagcg gtgcctttat aaatatatat
     4141 ccgccgggga taccgcttgt ggtgccggga gagataatga acgacaagct catagatgat
     4201 gtaataaagg ctgtaaattc aggtcttgaa gtggatggcc tggattttga tccgggtgcc
     4261 aatatgtgga agtttgttgc ggtgctgtaa gcaacagttt gacatagcaa acaatcatat
     4321 ttatactaat taaaatataa gaggtttaca gatgggtaag attttctgcg tgatgggaaa
     4381 aagcgcatca ggcaaggaca ctatatacaa taaaatactg caggatgatt ccctaatgct
     4441 tagcaggatt atcccgtata ccactcgccc gataagggac gga
//
LOCUS       DPUF01000161            5507 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3945, whole
            genome shotgun sequence.
ACCESSION   DPUF01000161 DPUF01000000
VERSION     DPUF01000161.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3945"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /locus_tag="DHV81_05635"
     CDS             complement(<1..2252)
                     /locus_tag="DHV81_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose phosphorylase"
                     /protein_id="HCI93958.1"
                     /translation="MAIMQEIETKLQKGISVSTDEEVYYALLELVKDKAEKKVSNKGK
                     KKLYYISAEFLIGKLLSNNLINLGIYDEVKETLEKNGKSLAEIEEIELEPSLGNGGLG
                     RLAACFIDSIATLGLNGDGVGLNYHYGLFKQVFENNLQKETPNPWITKESWLTKTDIT
                     YPVSFGGFTVQSRLYDIDVVGYNNRTTKLHLFDIESVDESIVGDTIDFDKDDIKKNLT
                     LFLYPDDSDDKGRLLRVYQQYFMVSNAARLILAEAEAKGSNLHDLADYAAVQINDTHP
                     SMVIPELIRLLQEKGILMDEAIEIVSKVCAYTNHTILAEALEKWPISFLEKAVPQLMP
                     IIRELDNKVRAKVADESTYIIKDGLVHMAHMDIHFGYSVNGVAYLHTEILKNTELNNF
                     YKLYPEKFNNKTNGITFRRWLMSCNPELSAYITELIGDGWKKDANELEKLGNFINDDT
                     VLTKLVDIKNAKKTELASYLKKTQNLDVPDNSIFDIQVKRLHEYKRQQLNVLYIIRKY
                     FEIKAGKKPSTPITCFFGAKAAPAYIIAKDIIHAILCLQQIINNDPEVSPYLKVFMVE
                     NYNVTLAEKLFPAANISEQISLASKEASGTGNMKFMLNGAITLGTSDGANVEIAELVG
                     DENIYVFGEDSQTVIDRYERGDYCSKDYYDKDADLKKAVDFLVSDEMMNVGSKENLER
                     LYNELLNKDWFMTFPDFEDYCKTKEKAYADYEDKKAWAKKMLVNISKAGFFSSDRTIK
                     QYNDEIWHLEA"
     assembly_gap    2388..2588
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2589..3335
                     /locus_tag="DHV81_05640"
     CDS             <2589..3335
                     /locus_tag="DHV81_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI93959.1"
                     /translation="MELIYIKKGCGHISVDFKEYHVTAPSLVLILPGQLHSIDQYMDE
                     SMEYENIIFSLSMLLPGKYDYTKEDFLTPLLAGRFTVPTVFTPVYPYYEDVVAPIDAC
                     DEICKTMPQGYQLYIKSKLYEFFFVLDNRCRNLTKPLKSKKTLDKMKVVLKYVENNYA
                     DKISIADIADVAGFSESHFMRYFKETMGTSFVDYLKDYRLTMAARLLQSSDSSVLSIS
                     GEVGFESLSYFNRAFKKHYGMTPLQYRKGS"
     gene            3392..3907
                     /locus_tag="DHV81_05645"
     CDS             3392..3907
                     /locus_tag="DHV81_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="HCI93960.1"
                     /translation="MKKNTYIKMTAPFRENNRAARKLELINKICTYLVFITYPVYVLY
                     LCFTAGHMLALSIIAPLIGFIAVSVFRYIVNRPRPYEKFDMPPVIPKDTLGRSFPSRH
                     VFSAFIIAFTVLICSPAASPLWFTGILLAVLAAIIACVRVISGVHFISDVVGAFVFAV
                     IEAYIVTVFFL"
     assembly_gap    4071..4120
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4121..4335)
                     /locus_tag="DHV81_05650"
     CDS             complement(<4121..4335)
                     /locus_tag="DHV81_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93961.1"
                     /translation="MKKMKYLLALCLILAAASAFAACGNNSNVADTSMVENTQRGGSA
                     ADDAGNAGKDIIDGAGNAGKDVIDGAG"
     assembly_gap    4452..4471
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4472..4809)
                     /locus_tag="DHV81_05655"
     CDS             complement(<4472..4809)
                     /locus_tag="DHV81_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HCI93962.1"
                     /translation="MDYVKAFVVGGIICAIVQILMEKTKLMPGRIMVMLVCTGALLGA
                     VGIYGPFSKWAGAGANVPLLGFGNVLWKGVKEAVDSEGFIGLFKGGFKACAVGVSAAL
                     IFSYIAALIT"
     gene            complement(4929..>5180)
                     /locus_tag="DHV81_05660"
     CDS             complement(4929..>5180)
                     /locus_tag="DHV81_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HCI93963.1"
                     /translation="ISKNHEDCGMKMFDATNQNTGSGGSGCACSATVLSAYYLPKLRS
                     GELKRILFVPTGALLSPVSFNEGESVPGIAHGVVLEAVM"
     assembly_gap    5181..5311
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5312..>5507)
                     /locus_tag="DHV81_05665"
     CDS             complement(<5312..>5507)
                     /locus_tag="DHV81_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HCI93964.1"
                     /translation="GSAAFIVGSGAGGNEMARCDNADSISRKVQIEGITTGVVVDYGI
                     KDAMNMGAAMAPAACELIVN"
BASE COUNT         1304 a         1219 c          991 g         1591 t
ORIGIN      
        1 gcctctaaat gccagatttc atcattgtac tgcttgattg ttctgtcaga agagaagaaa
       61 ccagccttgc tgatgttgac aagcatcttc ttagcccaag ccttcttatc ctcataatca
      121 gcgtaagcct tctcttttgt cttgcagtaa tcctcaaagt ctgggaatgt catgaaccag
      181 tctttgttga ggagctcgtt gtaaagtctc tctaagttct ccttagaacc aacattcatc
      241 atctcatcac ttacaaggaa gtcaacagcc ttctttaagt ctgcatcctt gtcatagtaa
      301 tccttagagc agtagtcgcc tctctcatat ctgtcgataa cagtctgtga atcctcaccg
      361 aatacataga tgttctcatc gccgacaagc tcagctatct ctacgtttgc tccatcactt
      421 gttccaagtg taatagcacc gttaagcatg aacttcatgt ttcctgttcc tgatgcctcc
      481 ttagaagcaa gagaaatctg ctctgaaata tttgcagccg ggaataattt ctcagctaat
      541 gttacgttgt agttctcaac catgaatacc ttaaggtatg gacttacctc aggatcattg
      601 ttgataatct gctgtaagca gaggattgca tggatgatat cctttgcaat gatatatgca
      661 ggagctgcct ttgcaccgaa gaagcatgtg attggtgtag atggcttctt gcctgccttg
      721 atctcaaagt acttacggat gatgtaaagc acgttgagct gctgtctctt gtactcgtga
      781 agtctcttaa cctggatatc aaagattgaa ttgtcaggta cgtcgagatt ctgagtcttc
      841 tttaagtaag aagcaagctc tgtcttcttt gcgttcttga tgtcaacaag ctttgtaaga
      901 acggtgtcat cattgatgaa attgccaagc ttctctaact cgtttgcatc cttcttccag
      961 ccgtcaccga taagctctgt gatgtatgct gaaagctccg ggttacaaga cattaaccaa
     1021 cgacggaatg tgataccgtt tgtcttgttg ttgaacttct caggatataa cttgtagaag
     1081 ttgttgagct ctgtattctt taaaatctct gtgtgaaggt atgcaacacc gtttacagaa
     1141 tatccgaaat gaatatccat gtgtgccatg tgtacaagac cgtccttgat gatgtatgtg
     1201 ctctcatcag ctacctttgc gcgaaccttg ttgtcaagct cacggatgat tggcataagc
     1261 tgtggaactg ccttctccaa gaagctgatt ggccattttt caagagcctc tgcaaggatt
     1321 gtgtggttag tgtatgcaca aacctttgat acaatctcaa tagcctcatc catcaggatg
     1381 cccttctcct gaaggagacg gataagctct ggaataacca ttgatggatg tgtatcgtta
     1441 atctgaactg ctgcataatc agcaaggtca tgtaaattag agccctttgc ctctgcctct
     1501 gcaaggataa gtcttgctgc gtttgatacc atgaaatact gctggtatac acgaaggaga
     1561 cgtcctttat catctgaatc atcaggatat aagaataatg taaggttctt cttgatatca
     1621 tccttgtcga aatcaattgt gtcgcctacg attgactcat caactgactc gatgtcgaat
     1681 aagtgaagct ttgttgtacg gttgttgtat ccaactacgt cgatatcgta aagtcttgac
     1741 tgtactgtga atccgccaaa gcttacagga tatgtgatat ctgtctttgt aagccagctc
     1801 tcctttgtaa tccatgggtt tggagtctcc ttctggagat tgttctcaaa tacctgcttg
     1861 aataatccgt agtggtagtt gagacctaca ccgtctccgt tgagcccgag tgttgcgatt
     1921 gaatctataa agcatgctgc aaggcgtcca agtccaccat ttccaagtga aggctcaagc
     1981 tcgatttcct cgatttcagc aagtgacttt ccgttcttct caagagtctc tttaacttca
     2041 tcgtagatac ctaagttgat aaggttgttt gataaaagct ttccgataag gaactctgct
     2101 gagatgtagt agagcttttt ctttcctttg tttgaaacct ttttctctgc cttgtctttt
     2161 acaagctcaa gtaatgcgta gtatacttcc tcgtctgtac taacgctgat tcccttctga
     2221 agctttgttt cgatttcctg catgattgcc attctgattt cctcccaaat ctgttatcat
     2281 gtgtacacgc caatgtgtca aattaactaa ttttcattta gcgtgcttaa cttccttgtg
     2341 tcatttgatg aatcaagtat aatccattac actgcacaat tcttgcannn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnat ggagcttatc tatataaaga agggctgcgg tcatatatct gtggatttta
     2641 aggagtacca tgtcacagcg ccaagccttg tgctgatact gccgggacag cttcactcta
     2701 tagaccaata tatggatgaa tcgatggagt acgaaaatat catattttcc ctgtcgatgt
     2761 tgcttcccgg aaagtacgat tacaccaagg aggattttct tacacctctc ttagccggca
     2821 gattcacagt tccgacggtt tttacaccgg tgtatccata ctacgaggat gtcgtggctc
     2881 caatagatgc atgcgatgag atttgcaaga cgatgccgca gggctatcag ctttatataa
     2941 aaagcaagct ctacgaattc ttttttgtgc ttgataaccg ctgccgtaat ctgacaaagc
     3001 cactaaaaag caaaaagact cttgataaga tgaaggttgt gctcaaatat gtggaaaata
     3061 attatgccga caaaatctcc attgctgata tcgctgatgt agccgggttt tccgagtctc
     3121 attttatgag gtattttaaa gaaaccatgg gcacatcgtt tgttgactat ttaaaggact
     3181 accgcctgac catggctgcg agacttttgc aaagctcaga cagctcggtg ctttctattt
     3241 ccggtgaagt ggggtttgaa agcctgtcgt attttaacag agccttcaaa aagcactatg
     3301 gcatgacacc tctgcagtac cgcaagggca gttgacacca ctctagacag ttacaataca
     3361 atatttcata aataaacgat ggaaaataat catgaaaaaa aatacataca taaaaatgac
     3421 tgccccattc cgtgaaaata acagggcagc ccgaaagctt gaattaatca ataaaatatg
     3481 cacttattta gtttttatca cataccctgt atacgtgctg tatctgtgct ttacagccgg
     3541 tcatatgctt gcactctcaa tcattgctcc gcttatcggc tttattgcag tatctgtttt
     3601 cagatatatc gtaaacagac cacgtccata cgaaaaattt gatatgccgc ctgtcattcc
     3661 aaaggatacc ctcggaaggt cttttcctag caggcatgtt ttttctgcat ttataattgc
     3721 atttactgtt ttaatatgct cacctgctgc ctcgccttta tggttcaccg gcattttgct
     3781 tgcagtcctt gcagctatca ttgcctgtgt ccgtgtgata tcaggtgtgc attttatcag
     3841 cgatgttgtc ggtgcttttg tttttgcagt cattgaagca tatatagtga cggttttctt
     3901 tttataaata gctgttagtt gtttccacca tcaacactct tttttatggc atttccggca
     3961 tcatctacgg catcctttgc gccgtctatt acatccttgc cggcatcacc tgcgccgtct
     4021 atcacatctt ttccggcatc gcctgcgccg tctatcacat cttttccggc nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgcctgctc cgtctatcac
     4141 atccttgccg gcatttccgg cgccatctat gatatccttg ccggcattcc cggcatcgtc
     4201 ggcggcagag ccacctcttt gtgtattttc aaccattgag gtgtctgcca cattactatt
     4261 gtttccacag gctgcaaaag cgcttgcagc agcgagtatc aggcaaagtg ccaataaata
     4321 tttcattttc ttcattgtat cagtctcctt tttgttttgg agatagtatg ggcaattttt
     4381 cgtaatttat acatcattcg taactattct gaaccactga gaacaaaaaa gctaatgcgt
     4441 catgcatgca tnnnnnnnnn nnnnnnnnnn nttcgtgatg agtgccgcga tatatgaaaa
     4501 gataagtgct gccgatactc cgaccgcaca agccttaaag ccgcccttaa acagacctat
     4561 aaatccctcg ctgtcaactg cctccttcac acccttccac agtacattgc caaagcccaa
     4621 aagcggtacg tttgcaccgg cacctgccca ctttgaaaat ggtccgtata tgccgacggc
     4681 gccaagcagc gctcccgtgc acacgagcat taccataatt ctgcccggca tgagctttgt
     4741 tttttccatt aaaatctgta cgatggcgca gatgatacca cccacgacaa atgcttttac
     4801 ataatccatt atatccttac gcctttcttc tatttttgaa cgtgcagccc atccctcaaa
     4861 aacggctcgt tcaccctatc tcgttcaccc tattatatat gtgaagccat cagcttcaaa
     4921 ttacaacttc acattacagc ttcaagcacc acaccgtgtg caattcccgg tactgactcg
     4981 ccctcgttga agctgacagg cgaaagaagt gctcctgtag gcacaaaaag tatacgcttc
     5041 aattcaccac ttctaagctt tggcagataa tatgccgaca aaacagttgc cgagcacgca
     5101 cagcccgaac cacctgagcc tgtgttctgg ttcgttgcat caaacatctt catgccacaa
     5161 tcctcgtgat ttttgcttat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgatttact atcagctcac aggccgcagg
     5341 agccatggcg gcgcccatgt tcatggcatc ctttatgcca tagtccacca caactcctgt
     5401 tgtgattccc tcaatctgca ccttccttga tatactatct gcattgtcac acctagccat
     5461 ttcattacca cctgctccac tgcccacaat aaatgctgca cttcctg
//
LOCUS       DPUF01000162            6116 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_25502, whole
            genome shotgun sequence.
ACCESSION   DPUF01000162 DPUF01000000
VERSION     DPUF01000162.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_25502"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="DHV81_05670"
     CDS             <1..1095
                     /locus_tag="DHV81_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCI93965.1"
                     /translation="KNIITMLLIFLTAGAFVGVVGRSSAESVAYFMLSLIPARFAVMV
                     LFVVACFVSIAMGTSVGTITLIVPIAAAVSDASGFGLPLCIGSVMGGAMFGDNLSFIS
                     DTTIAACNGQGCQMKDKFRENFFIALPAAVMTLVVIAILSFRTDIAGAVSQEYHLLQI
                     VPYILVLFGGIAGINVFVVLIIGIISGTVIMLATGNTAPYDLLTNMGSGASGMFETCM
                     VAVLVAAMCALIREYGGFDALLSGIYRTFRGKRGGLLGMGLLVGLIDIATANNTVAIV
                     MANPIAKEMAQKYDITPRKTASILDTFSCIFQGMIPYGAQMLVAISAVHELGHDVSAF
                     NILPYLLYPMFLLVSSLVVVFVVENGRKFN"
     gene            complement(1186..1413)
                     /locus_tag="DHV81_05675"
     CDS             complement(1186..1413)
                     /locus_tag="DHV81_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger domain-containing protein"
                     /protein_id="HCI93966.1"
                     /translation="MKCFLCKGDTVKSTTTYMTAYKNCYIIIKNVPCQKCSQCGEEFI
                     NGSTMQKIESIISKLKSMLPEITVIDFQNAA"
     gene            complement(1418..1735)
                     /locus_tag="DHV81_05680"
     CDS             complement(1418..1735)
                     /locus_tag="DHV81_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HCI93967.1"
                     /translation="MCLTIDELRGLCTPDNIYLTLHASKRLEQRGILIDDVISCIMTG
                     EIIEEYPNDYPYPSCLTLGNLNTKCPLHVVVGSNHEQLWIITAYYPSSDKWESDLKTR
                     KEN"
     gene            2199..3047
                     /locus_tag="DHV81_05685"
     CDS             2199..3047
                     /locus_tag="DHV81_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCI93968.1"
                     /translation="MPENSSCSSAGSCTKSSCEGCPSHNGGPQSFLVEQNKFSNIKHV
                     IGVVSGKGGVGKSFVTSSLAVNMAKKGYKVGILDADITGPSIPKMFGAHDQILGDENG
                     LMHPYETKEGIKLISVNLLMDNEEDPVIWRGPVIAGVVKQFWNETAWGDIDYLFVDMP
                     PGTGDVPLTVFQSLPVDGIVIVTSPQELVNMIVKKAYNMAEAMHITVLGVVENFSYLK
                     CPDCGKEIKLFGESHIDEIAKELSVPVLGKLPLDTSYAAIADKGDFYSIENDHLAKAT
                     EVLERI"
     gene            3493..5625
                     /locus_tag="DHV81_05690"
     CDS             3493..5625
                     /locus_tag="DHV81_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCI93969.1"
                     /translation="MKKIFDVLNVIKQKLFVKKDKIHSEKYYRRIDFLNKYSLLFHAI
                     IAMAIVFIVEIISRRSFISACKFVDAHTLAFMYNSFLVFVSFSLVYLFRRRAFARVII
                     TGFWTILGIINGCVLSNRVTPFGYTDLKCIPELLAMNNTSYFTAQQATIVVVGLGAFA
                     LFLVALFIKGPKYTGKIRYAGVSVAFLALLFVAIPVTTNVAQNTNVVASYYSNIAQGY
                     DDYGFVYSFSSTVVDRGMKKPEDYNKQNVEDVEQKVNSQKQTTTVDGKTGPNIICVLL
                     ESFCDPDEINFLQVNEDPIPTFHELEKNYSSGYLNVPVVGAGTANTEFEMLTGLSMQY
                     FGTGEYPYKTILKQTDCESIASDLSKIGYATHVVHNNGGNFYSRTNAFSKMGFDTFTS
                     KELMNITEYTPNGSWPTDDILVSEAMKTFDATPDQSDFTYIITVGTHGDYPKEPVIEN
                     PTYTVSGVEDEGMKNAWTYYVNQLNEADRFIKELTDELSKRDEDTIVVMFGDHLPTMG
                     LQDSDMKSGDIYKTKYITWNNMGLPKEDADLYAYQLLAQTTDTVGIHEGTIMNYHQTQ
                     MNSTDEASYQDGLDLLQYDILYGKRYCYNGTDLYPASDLVMGIDKVDITNVSDSSTGD
                     TVYIYGHNFTNWSKVYINDSKVASTYLSAGVLAIRKEDISDGDEITVCQVGSSDTIFR
                     KSENAYTYVDPAVEHDSESETDEPTENQ"
     assembly_gap    5862..5976
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1788 a         1145 c         1372 g         1696 t
ORIGIN      
        1 aaaaatatca taacaatgct gctcattttc cttaccgcgg gagcattcgt gggtgtagta
       61 ggtcgaagca gtgcggagag cgtggcatat ttcatgctgt cactgatacc ggcaaggttc
      121 gcggtgatgg tgctttttgt tgtggcatgc tttgtgtcta ttgcgatggg gacatcggtt
      181 ggaaccataa cactgattgt gccgatagcg gcagcagtat cggatgcatc gggttttggt
      241 ctgccgcttt gtataggctc cgtaatgggt ggcgcgatgt tcggagacaa cctttccttt
      301 atatcagata cgaccattgc cgcatgtaac ggacagggct gtcagatgaa ggacaagttc
      361 agggagaatt tttttattgc acttccggcg gcagtaatga cgctggttgt tatagcaatt
      421 ctgtcattca ggacagatat cgcgggcgct gtatcgcagg aatatcatct gttgcagata
      481 gtaccgtata ttctcgtgct gttcggtgga attgccggaa taaatgtatt tgtcgtgctt
      541 ataataggaa tcatatcggg aacggtcata atgcttgcaa cgggaaatac agcaccatac
      601 gatctgctta ccaatatggg aagtggagcg tcaggcatgt ttgaaacctg tatggttgca
      661 gtgcttgtgg cagccatgtg tgcgctgatc cgtgaatatg gtgggtttga tgccctgctt
      721 tcgggtattt acaggacgtt caggggcaaa cgaggtggac tgctcggtat gggacttctg
      781 gtaggactta tcgatattgc cactgccaac aacacagttg ctatagttat ggcaaatccg
      841 atagcaaagg aaatggctca gaaatatgac ataaccccac gtaaaacagc gtctatactg
      901 gatacatttt cgtgcatttt tcaggggatg ataccatatg gagcgcagat gctcgtggcc
      961 atatcggcag tacacgagct ggggcatgat gtgtcagcct ttaatatact gccgtatctg
     1021 ttgtatccga tgtttctgct ggtaagctcg ctggtggtgg tttttgttgt tgaaaatggg
     1081 agaaaattta attgaaaaag aagtttaaag cgaaaaaata atgaggttat aaatgaaatg
     1141 ccggatagaa atatgatatt tcatccggca tttattatgt gaaatttagg ctgcattttg
     1201 aaagtcaatt acagttattt ctggtagcat ggattttagt tttgatataa tggactcgat
     1261 tttttgcatt gtggagccat taataaactc ttcaccacac tgtgaacatt tttggcatgg
     1321 aacatttttt ataataatat aacagttttt atatgcagtc atataagttg tggtactttt
     1381 gacagtatca cctttgcaca ggaagcattt cataatatca attctccttt cttgttttta
     1441 aatcggactc ccatttgtct gaagacgggt agtatgcggt gataatccag agctgttcgt
     1501 gattggaacc gactacaaca tgtaatggac attttgtatt cagatttcca agtgttaagc
     1561 aactgggata tggatagtca ttagggtatt cctcaattat ttcaccggtc atgatacatg
     1621 atatgacatc gtcaatgagg ataccacgtt gctcaagtct ttttgacgca tgcagtgtta
     1681 aataaatatt gtcgggagta cacaaacctc taagttcatc aattgtaaga cacatctgtt
     1741 attctcctca tatgaattaa ttataattat agtagtattt ggagagatta gcaatgctat
     1801 tctaaaaatt cactaattta cattaattaa ccttaattaa ctccttgaca atcatttatg
     1861 acaagaagct cgattatgtt ataaagctgc gtgaatatcc acataataaa aagggaaaac
     1921 gtagcacgat tattgatatg tagtgacaaa acatgaaaag gtggctgaat ttccgggaaa
     1981 tagtcacgaa ataaatacga ttaaacaggt gggaataggg gtgtttttga aaaaaagcat
     2041 aaaaaatact gcatacgtgt tatactatat tatatagatg tcgtatgaaa tatgaacgat
     2101 aaacacataa ataaacacag tataggagaa gtaatcgcaa tcacaaatat attttgtgaa
     2161 atacaacaat tgctttctca taggaaaagg agataaaaat gccggaaaac agtagctgta
     2221 gcagcgcggg aagctgcaca aaatcaagct gcgagggctg tccatcacat aatggcggac
     2281 cacagagctt cctcgtagag cagaataaat tttcaaacat caagcatgta ataggagttg
     2341 tcagcggaaa gggcggagta ggaaaatcat tcgtcacatc ctcactggcg gtaaacatgg
     2401 caaagaaggg ctacaaggtt ggtatccttg atgccgatat cacaggaccg tcaataccaa
     2461 agatgtttgg cgcacatgac cagatactcg gagacgaaaa cggcctgatg catccgtatg
     2521 agacaaaaga gggcatcaag ctcatctcag taaacctgct catggacaac gaggaggatc
     2581 cggttatctg gagaggccca gtcattgccg gagtagtaaa acagttctgg aacgagacag
     2641 catggggcga tatagactat ctattcgttg atatgccacc gggaacagga gatgtacctc
     2701 tcaccgtatt ccagtccctc ccggtagatg gaatcgtaat cgtcacatca ccacaggagc
     2761 ttgtcaatat gattgtcaaa aaggcctaca acatggcgga ggcaatgcat atcacagtgc
     2821 ttggtgtggt agaaaacttc agctatctca aatgtcctga ctgcggcaag gagataaagc
     2881 tttttggaga gagccatata gacgagattg ccaaagagct ttcagtaccg gtacttggca
     2941 agctgccact cgatacatca tatgcagcta tcgccgataa gggtgatttc tatagcatag
     3001 aaaacgacca cctcgcaaaa gcaaccgaag tacttgagcg catttaaata tttcgtaatg
     3061 aagcaggcat atatccgccg ggtgcggtat atatgcctgt atttaataga aaacctttaa
     3121 gcataattcg tacagaatct taatcgcata gcgtagctca cgggtatgca gtctgcggca
     3181 catgaatccg cggcattcgc cttgcactgc caaatattaa actttcttta aatatgcgat
     3241 tcccctattt acatttcgtt tcattggctt cataatcata ttacccacgg attaaacaat
     3301 agttttcata ctcaaaattt acggaaacaa ccaaaaaagg taaaaagagt taattttatt
     3361 atcaatattt acccaaaaat aataatttta acgtcaattt ttataaaaaa atgcccttgt
     3421 ttttgtgcat atttttgtct atattatcat gtagtaaaga gaaggtaaaa gaaaggcaaa
     3481 taggaggaaa atatgaagaa aatatttgat gtattgaatg tcattaaaca aaagctcttt
     3541 gtaaagaaag acaagattca ttcagaaaaa tattatcgca gaattgattt tttaaataaa
     3601 tattcgcttt tattccatgc aatcatagct atggctatcg tatttatagt ggagattatt
     3661 tcgcgcagga gctttatctc tgcatgtaag ttcgtagatg cacatacact tgcgttcatg
     3721 tacaactcat ttttagtgtt tgtatcattt tcacttgtgt atctgttcag gcgcagagct
     3781 tttgcaaggg taataattac aggcttttgg actatcctcg gaatcatcaa cggctgtgtg
     3841 ctttcaaacc gtgtcacacc gtttggttat actgatttaa agtgtattcc ggagctttta
     3901 gccatgaaca ataccagcta tttcacagcg cagcaggcta caatagttgt agttggactt
     3961 ggtgcatttg cactgtttct tgtggcactt tttatcaagg ggcctaaata tacaggcaaa
     4021 atccgttatg caggtgtttc agtggcattt ttagcactgc ttttcgtggc aatccctgtc
     4081 acaaccaatg tggcacagaa taccaatgta gttgccagct actattcaaa catcgcccag
     4141 ggctatgatg attatggctt tgtatactca ttctcatcta ctgtagtgga ccgtggaatg
     4201 aaaaagccgg aggactacaa taagcagaat gtggaagatg tagagcagaa ggtcaattct
     4261 cagaagcaga ccaccacagt ggacggaaag acaggtccaa acatcatctg tgtgctttta
     4321 gagtcattct gtgacccgga cgagattaat ttccttcagg tgaatgagga tcctattcct
     4381 acattccatg agcttgagaa aaactattca agcggatatc tgaatgtacc tgtagtcgga
     4441 gcaggaactg ctaacacaga gtttgagatg cttacagggc ttagcatgca gtatttcggt
     4501 acaggagagt atccatacaa gactatctta aagcagacag attgcgagag cattgcctct
     4561 gatttatcta agattggata tgcaacacat gtagtgcaca acaacggtgg taatttctac
     4621 agccgtacca atgcattttc taagatgggc tttgatacct ttacaagtaa ggagctcatg
     4681 aatatcacag agtacactcc aaacggaagc tggcctacag atgatattct cgtcagcgag
     4741 gctatgaaga cctttgatgc tacaccggat cagtctgatt ttacttatat catcacagtt
     4801 ggcactcacg gtgactatcc aaaggagcct gttattgaaa atcctacata caccgtatca
     4861 ggtgttgagg acgagggcat gaaaaatgcc tggacatatt atgtaaacca gctcaacgag
     4921 gctgaccgct tcatcaagga gctcacagat gagctttcta agagagatga ggacaccatc
     4981 gttgttatgt ttggcgatca cctccctaca atgggacttc aggattctga tatgaagtca
     5041 ggtgatatct acaagacaaa gtacatcaca tggaacaaca tgggactgcc aaaggaggat
     5101 gctgaccttt atgcttacca gcttcttgcc cagactacag acactgtagg tatccatgag
     5161 ggaactatca tgaactatca tcagactcag atgaacagta cagatgaggc atcatatcag
     5221 gatggacttg acctgttaca gtacgatatc ctttatggaa agcgctactg ctacaacggc
     5281 acagatttat atccggcttc agatcttgtc atgggaattg acaaggtgga catcacaaat
     5341 gtgtctgatt catcgaccgg tgatacagta tatatttacg gtcacaactt taccaactgg
     5401 agtaaggttt atatcaacga ctccaaggtg gcttcgactt atctctcagc aggtgtgctt
     5461 gctatcagga aggaagacat cagtgatggt gatgagataa ctgtgtgcca ggttggatct
     5521 agtgatacca ttttcagaaa gtctgagaat gcgtatacat atgtggatcc tgcagtggag
     5581 cacgacagtg agtcggagac agatgaaccg acagagaacc agtagatttt aatgaatttt
     5641 ttcttgggtg gacggggcgc aggtcccggg tcccagtgtg gagtggcgac atgtactctc
     5701 gcgttcgcta cgcttcgcag ccaagattct atacgaaatt atgcttttag ggtttctatg
     5761 cttgacgaaa ccggattata tgcgccgtcc atggcgcata taatccttgc cccgccatcc
     5821 ttggcggggc atttctgcgt tgtgcgccta gcggcgcacg tnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgaa ggaagctgga tgtgaggaat
     6001 atactaactc acgggcaaat ctctggtctg acgaacagaa atctcatatg aggtcatgca
     6061 tgtgggtaag gcagcaagcc atgttgaagc ccgataacta cacggaatca tgcatg
//
LOCUS       DPUF01000163           16580 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10721, whole
            genome shotgun sequence.
ACCESSION   DPUF01000163 DPUF01000000
VERSION     DPUF01000163.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 16580)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16580)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16580
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10721"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="DHV81_05695"
     CDS             <1..465
                     /locus_tag="DHV81_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCI93970.1"
                     /translation="DPTNYADTAAQQRNSIWAIGSGQLFGKGLNNSVVTSMKNTNYII
                     EPQTDFIFAVAGEELGFIGTISIIILLLLIVIECILIARKAKDTSGKLICCGMGALIG
                     FQAFINLCVATGLMPNTGMTLPFVSYGLTSLVSLYMGMGIVLNVGLQPKKYS"
     gene            477..872
                     /locus_tag="DHV81_05700"
     CDS             477..872
                     /locus_tag="DHV81_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCI93971.1"
                     /translation="MNIGLVAHDSKKKLMQNFCIAYRGILSKNNLYATGTTGRLIEEV
                     ANLSVHKYLAGHLGGIQQLGAQIEHNDIDLVIFLRDPLKPKPHEPEVNSIFRICDEHN
                     IPLATNLATAELLILSLDRGDLDWREMYK"
     gene            922..1827
                     /locus_tag="DHV81_05705"
     CDS             922..1827
                     /locus_tag="DHV81_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCI93972.1"
                     /translation="MTGCGTKIENAYDTYSTDYINGYNGSNFFASNLCVTNDVNFGQD
                     NMQFAKTAEGAGAFNIDTHQVLYSQNLFEKLYPASTTKILTAYIILKNCDLNATVTVS
                     HDAANPGHSSSVCGLKEGDVITVQDLLYGLLLESGNDAAIALAEYCSGSVEEFAKLMN
                     STAKSFGATNSSFVNPSGLPDENHYTTIYDMYLIFSNAISLESFVSIISSQTHDVAYT
                     NAQGAAVSKTFKNTCGYLTGAYTAPENVTVTGGKTGTTGEAGHCLVLLSQNAKNERII
                     SIVYKADGKKDLYSFMNEILSGFAN"
     gene            1923..2345
                     /locus_tag="DHV81_05710"
     CDS             1923..2345
                     /locus_tag="DHV81_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HCI93973.1"
                     /translation="MIEIICGDKGKGKTKELLDKVNSSIKGNEGSIVFLDKSQKHMYE
                     LNNQVRLINVMDYPIDNCDEFLGFICGIISQDHDLEEMYLDSFLTIASLDDEGDITKA
                     IEKLDIISEKFKVKFILSVSRNIDKLPECAKAKVIISL"
     gene            2399..3787
                     /locus_tag="DHV81_05715"
     CDS             2399..3787
                     /locus_tag="DHV81_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93974.1"
                     /translation="MSRKNKKVLKYRTGFNLNIGFIVFFVIIIYVIFHIFTYFTSNPV
                     SEYEVQQGTIATNNIYKGMIIREESVVYAEESGNLNYFVSNGSKVATSDVVYSVDTDG
                     SIATQITGAQNDASAITDEDAGKIITDINSFSSGYNKRYFSKVYTFKNDLSAQLTQVL
                     SQNALTSLADTISTAEANNTFYTYKPTAPGIVYYGIDGYEDITTDSFTLDQYNNTNYE
                     ETDLTNNSTINQLDPVYKLITSENWNILINVPDNVAKSLNDKSVIKIRFCDDDYTTTV
                     SFSLLKKDDAFFLKLNMKNSLIRYINERFTNIELVLGADTGLKIPNSAITKKEFFTIP
                     KDYFTASGDSDDSSLMIKDKSSGSVNIVSPTIFSQNDDFYFVDDEYLKDGDIIVKPDS
                     SSTYRVGTDKDKLTGVYNINKGYAVFKQINVLASNDDYTIVEAKTPYGISLYDHIALD
                     GKSVKENQTITK"
     gene            3834..4532
                     /locus_tag="DHV81_05720"
     CDS             3834..4532
                     /locus_tag="DHV81_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCI93975.1"
                     /translation="MLKDNLHQVQQNIKAACDRAHRNYSDVTLIAVSKTKPVEMLSEI
                     YDEGIREFGENKVQEMCEKMEVMPKDIHWHMIGHLQTNKVKYIVGKTSLIHSVDSLHL
                     AKEIQKQAIKHDCSCDILVEVNIAGEASKFGTSRDEAISLVEEIAKLDHIHIKGLMTI
                     APFVDDPEDNRQYFRDIKQLSVDIAQKNIDNVSMNVLSMGMTGDYTVAIEEGATMVRV
                     GTGIFGERDYSKAN"
     gene            4582..5124
                     /locus_tag="DHV81_05725"
     CDS             4582..5124
                     /locus_tag="DHV81_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCI93976.1"
                     /translation="MSFIDKILDKMSLNSEDDEEFDNEDYYLDDEEEEELPRNPFRRK
                     KEAAEEEPAIKSTRRDTTPKEKPVKTTSKITPISKSSRKQVSSDMEVCVIKPSSIEDE
                     IEITDTLLNGRTVVINMEGLNVDVAQRIIDFTSGSAYALHGNLQKISNFIFIATPHGV
                     DISGDIQSLMDSFDLPGSSY"
     gene            5144..5749
                     /locus_tag="DHV81_05730"
     CDS             5144..5749
                     /locus_tag="DHV81_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569278.1"
                     /note="lipoprotein signal peptidase; integral membrane
                     protein that removes signal peptides from prolipoproteins
                     during lipoprotein biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HCI93977.1"
                     /translation="MKKNKTKKQMCTAGIIALILVAIDQLTKYLAVINLKQSSTGVFV
                     LIPGVFEFRYLENQSAAFGVDLLSIIQNIFHFTYWSENPLAFLRAKMIFFSVITIAVV
                     ILLCVWYRKIPVSKRFRLLNVVVILFVAGALGNLIDRIVNNYVVDFFYFSLINFPIFN
                     VADIYVTVAAFMFIILGLFYYKEEDFSLIFPDKKHDGKHEN"
     gene            5823..6761
                     /locus_tag="DHV81_05735"
     CDS             5823..6761
                     /locus_tag="DHV81_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HCI93978.1"
                     /translation="MNRDTFEVQSEQEGERLDKYLSSIYPDISRSFFQRILKENQILV
                     NDKPQKANYRLKTDDIVDVTIPDAVQTPILPQDIPLDILYEDDDVLVVNKPKGMVVHP
                     SAGHYSDTLVNAIMYHCKDSLSGINGEIRPGIVHRIDMDTTGSLIVCKNDESHIKISE
                     QIKDHSCNRIYVGIVCGNVKDDEGTIEGAIGRNPNDRKKMAINEKNGKPAVTHYKVLE
                     RFGDYTYMQFKLETGRTHQIRVHMASINHPLLGDMLYSSYSPTKNRFKDLEGQCLHAK
                     TIGFVHPKTGEYMEFDAPLPKYFANLLEVLYNINNK"
     gene            6818..7453
                     /locus_tag="DHV81_05740"
     CDS             6818..7453
                     /locus_tag="DHV81_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase"
                     /protein_id="HCI93979.1"
                     /translation="MGSNEIYTIGREFGSLGKQVGQDLAKRLGIKFYDKELLQKAAKE
                     SGLCEEIFESHDEKPTNSFLYSLVMDTYTGGSYSTAPFLDMPLNHKVFLAQFDAIKKI
                     AANESCVIVGRCADYALADEPHCLNIFIRSNMDDRIKRISERLNVPENKAKDIIKKKD
                     KERSSYYNYYTSKKWGDARSYDLCLNTSQISVEDSIELILKYRECMRNHNK"
     gene            complement(8300..9964)
                     /locus_tag="DHV81_05745"
                     /pseudo
     CDS             complement(8300..9964)
                     /locus_tag="DHV81_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
     gene            complement(10013..10210)
                     /locus_tag="DHV81_05750"
                     /pseudo
     CDS             complement(10013..10210)
                     /locus_tag="DHV81_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            10354..11376
                     /locus_tag="DHV81_05755"
     CDS             10354..11376
                     /locus_tag="DHV81_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing enzyme"
                     /protein_id="HCI93980.1"
                     /translation="MNYTECIENARPRLGKYCKACPECNGRACKNQMPGPGSKGIGDT
                     AVRNYDKWKQIRVNMDTIAENKPVDTSLSLFGRTFKYPVFAGPVGAVQLHYGDCLNDV
                     TYNDILVSACAKNGIAAFTGDGTDPNVMVAATNAIKNADGAGIPTVKPWNIETIREKM
                     ELVHESGAFAVAMDIDAAGLPFLKNLDPPAGSKTVSELCDIIQMAGTPFIVKGIMTVK
                     GALKAKEAGASAIIVSNHGGRVLDQCPATAEVLESIVKALEGSGIKILVDGGIRSGTD
                     VFKALALGADGVLIARPFVTAVYGGKADGVRAYIDKIGTELEDTMKMCGVSSLDEITR
                     DCVMTF"
     gene            complement(11407..11956)
                     /locus_tag="DHV81_05760"
                     /pseudo
     CDS             complement(11407..11956)
                     /locus_tag="DHV81_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12026..13258)
                     /locus_tag="DHV81_05765"
                     /pseudo
     CDS             complement(12026..13258)
                     /locus_tag="DHV81_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
     gene            complement(13281..14024)
                     /locus_tag="DHV81_05770"
     CDS             complement(13281..14024)
                     /locus_tag="DHV81_05770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93981.1"
                     /translation="MNNGTNNFQNFIGISSLQKTLRNALIPTETTQQFIVKNGIIKED
                     ELRGENRQILKDIMDDYYRGFISETLSSIDDIDWTSLFEKMEIQLKNGDNKDTLIKEQ
                     AEKRKAIYKKFADDDRFKNMFSAKLISDILPEFVIHNNNYSASEKEEKTQVIKLFSRF
                     ATSFKDYFKNRANCFSADDISSSSCHRIVNDNAEIFFSNALVYRRIVKNLSNDDINKI
                     SGDIKDSLKEMSLEEIYSYEKYGEFITQE"
     gene            complement(14482..16209)
                     /locus_tag="DHV81_05775"
     CDS             complement(14482..16209)
                     /locus_tag="DHV81_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCI93982.1"
                     /translation="MNKGKIRTRRLTIRAKILIPSIIIVVLVCGLMGYNSYTRFEKSM
                     VRMGVEEADMAATIVADSLDAKLVYKVTVGSEGTQVYQNLQGDLRKKQKACGIAFLYT
                     LYTDGKKVYYGVDSDEDAAKVGDEFADSYAELEPVFGGKEYIQDYIDHTEDGDLITVY
                     KPIEDNAGKVVAILGCDYDASSITAELQKAVVQTLQIGGICLILAILILTIIVSRITK
                     GLMQVNAKIYDLVHNEGDLTQKLDVRSGDELELIAGNVNELLAYIRKIMIGISSGSMR
                     LMSSSRKMVDHLSSADESITDVSATMQEMSAAMEETTSSLNQITEAIDEIYSSVERIA
                     GSADAGKVSSQEMESRASGANDAAAEGEKKANIETEKMAASLNEKIAKSKSVEQIEIL
                     TSNIIEITEQTNLLALNASIEAARAGEAGRGFAVVADEIGKLAGNSADAAAKIRQVSA
                     EVIQAVDELAEGSQQMIEFVRNSTEEGFGGLVATSENYATDANAMRAMMEQFAQTAEE
                     LRSTMDGIRESISAVNIAVEESAKGIAGVSESSVQLTGNVNDIQSEASDNNGIAEDLA
                     TEVGKFKLE"
BASE COUNT         5063 a         3552 c         3148 g         4812 t
ORIGIN      
        1 gacccgacaa attacgctga tacagcagca cagcaaagga actctatctg ggctataggc
       61 tcaggacagt tgtttggcaa gggacttaac aactctgttg taacctctat gaagaacacc
      121 aactacatca tagagcctca aacagatttt atatttgctg ttgccggaga ggagcttggc
      181 tttattggta caatcagtat aataattctt ttgcttctta tcgtaattga gtgtatactt
      241 atagcaagga aggcaaagga cacaagcgga aagctcatat gctgtggcat gggagcactt
      301 ataggctttc aggcctttat caatttgtgc gtagccacag gtcttatgcc caataccggt
      361 atgactcttc cctttgtcag ttatggactt acgtcacttg tttcactgta catgggaatg
      421 ggaattgtcc tgaatgtagg attacagccg aagaaatatt catagggagg ttagcaatga
      481 acataggatt agttgcacat gattcaaaga aaaaactgat gcagaacttc tgcatagcat
      541 acagaggtat cttaagcaaa aataatttgt acgccacagg caccacagga cgtctgatag
      601 aggaggttgc aaacctctca gtgcacaaat atcttgcagg acacttagga ggcatccagc
      661 agcttggagc acagattgag cacaatgaca tagatcttgt catctttctg cgtgatccat
      721 taaagcctaa gccacacgag cctgaggtaa acagtatttt ccgtatttgt gatgagcata
      781 atataccact tgccaccaat ctggctacag cagagctgct cattttatca ttggacagag
      841 gagatcttga ctggcgtgag atgtacaagt aaacaaagaa ttaaacgact gctatgtctg
      901 acatgcatag cagcggtttt tatgacaggc tgtggtacaa agattgagaa tgcatatgat
      961 acttattcca cagattatat aaatggatat aacggaagta atttttttgc gtccaacctt
     1021 tgcgtcacaa atgatgtcaa ttttggtcag gacaatatgc agtttgcaaa aaccgcagag
     1081 ggagccggag cctttaatat tgacactcat caggtgctgt atagccagaa tctgtttgaa
     1141 aagctatatc ctgcaagcac tacaaagatt ttaactgcat acataattct taaaaactgt
     1201 gatttaaatg cgacagtcac agtaagccat gacgctgcaa acccgggtca ttcatcatcg
     1261 gtatgcggcc taaaggaagg cgatgtcatt acagtgcagg acttattgta tgggctctta
     1321 cttgagagtg gcaatgatgc ggcaatagca ctggcagagt actgcagtgg ttctgtagaa
     1381 gaatttgcca agctgatgaa cagcactgca aaaagttttg gtgccacaaa cagcagtttt
     1441 gtcaacccaa gcggtcttcc tgatgaaaat cattacacta ccatatatga catgtatctc
     1501 attttttcca atgccatatc cttagagagc tttgtttcaa tcatcagttc acagacccat
     1561 gatgtggcat atacaaatgc ccagggcgcg gctgtttcaa agacctttaa gaatacctgc
     1621 ggctatctga ccggagcata tacagctcct gaaaacgtca cagtgacagg aggaaagaca
     1681 ggcactacgg gggaagccgg ccattgcctc gtactgctaa gccagaatgc taaaaatgag
     1741 cgcatcataa gcatagttta caaggcagat ggtaaaaagg atctttatag cttcatgaat
     1801 gaaatactta gtggatttgc taattaaata ttgagtttta tggttattta tactataata
     1861 atataaaaat acaattattt tttattaata atcacaatat ttatccatag gaggaatgag
     1921 ccatgataga aattatttgt ggagacaagg gcaagggcaa gacaaaagag ctgttagaca
     1981 aggtaaattc atcaattaag ggaaatgaag gttccatagt ttttcttgat aagagccaga
     2041 agcacatgta tgagcttaat aaccaggttc gactaatcaa tgttatggac tatcctattg
     2101 acaattgtga tgaattttta ggatttatat gcggaatcat atcgcaggat catgatctgg
     2161 aggagatgta tttagacagc ttccttacaa ttgcatcact tgatgatgag ggggatatca
     2221 ccaaggcaat cgaaaagctt gatataatca gcgagaagtt taaggtgaag ttcatcttaa
     2281 gtgtttcaag gaatattgac aagttgcctg aatgtgccaa ggcaaaggtt atcatttctt
     2341 tataaatgat taattagggg gatgtgtatt tcacatccct tttttaagga gtaaaattgt
     2401 gtcccgaaag aataaaaaag tactgaaata tcgcaccggc ttcaatctga atatcggttt
     2461 tatagtgttt tttgtaataa taatctatgt gatttttcac atatttacgt atttcacctc
     2521 aaacccggta tctgaatacg aggtacaaca gggtactata gcaaccaaca acatatataa
     2581 gggcatgata atccgcgagg aaagtgtagt gtatgctgag gaatcaggaa acctcaacta
     2641 ctttgtgtca aacggctcaa aggttgcaac aagtgatgtt gtctactctg ttgatacaga
     2701 tggctccatt gcaacccaga ttaccggtgc gcaaaatgat gcttccgcca tcactgacga
     2761 ggatgccggg aagattatca ctgacataaa cagcttttca tccggctaca acaaacgata
     2821 cttttcaaag gtttatacct tcaaaaacga tctgtctgca cagctgacac aggttctcag
     2881 tcaaaatgca ctgacaagcc tggcagatac tatcagtact gctgaggcta acaacacctt
     2941 ctatacatat aagcctacag ctcccggaat agtctattac ggaatagacg gctatgagga
     3001 tatcactaca gacagcttca ctctggatca gtataacaac actaattatg aagagactga
     3061 tttaaccaac aacagcacca tcaatcagct ggatcctgtt tataagctga ttacaagtga
     3121 gaactggaat atactcataa atgttcctga caatgttgcc aagagtctga atgacaagtc
     3181 agtcataaaa atcagattct gcgatgatga ctatacaacc acggtatcgt tcagcctttt
     3241 gaagaaggat gatgcttttt tcctgaagct gaatatgaaa aactcgctca tccgttacat
     3301 aaatgagcgt tttaccaata tagagctcgt gcttggagca gacaccgggc ttaaaatacc
     3361 taattcagct ataaccaaaa aggaattttt cactattcct aaggattatt tcactgcaag
     3421 cggcgattcc gatgattcat cgctcatgat aaaggataaa agcagtggct cagttaatat
     3481 agtcagtcca acaatattca gccagaatga tgacttttat ttcgtagatg atgaatatct
     3541 taaggacggc gatatcattg taaagcctga ttcttcgtca acctacaggg tgggaacaga
     3601 taaggacaag ctcacaggtg tctacaatat caacaaagga tacgctgtgt ttaagcagat
     3661 aaatgtactt gcatcaaacg atgactacac aattgtagaa gccaaaacgc catatggcat
     3721 ttcgctttat gaccatatag ccctggatgg caaatccgtg aaggaaaatc agaccataac
     3781 caaataatta ttggtaataa atattgataa aatatacaga agggacagat tttatgttaa
     3841 aagacaactt acatcaggtt cagcaaaata taaaggccgc atgtgacaga gctcacagaa
     3901 actatagtga tgtcacacta attgcagtca gcaaaaccaa acctgttgag atgctcagtg
     3961 agatatacga tgagggcatc cgtgagttcg gcgagaataa ggtacaggaa atgtgtgaaa
     4021 agatggaggt tatgccaaaa gatattcact ggcacatgat tgggcatctt cagaccaaca
     4081 aggtgaagta tatagttgga aaaacttcac ttattcactc tgtagattca ctgcatctgg
     4141 caaaggagat acaaaagcag gccataaagc atgactgcag ctgcgacata ctggttgagg
     4201 tcaatatagc aggagaggca tctaagtttg gcacatcacg agatgaggct atctctttag
     4261 ttgaagaaat tgctaaactg gaccatatcc atataaaagg acttatgact atagcaccat
     4321 ttgtagatga tcctgaggat aacagacaat actttagaga tattaagcaa ttatcagttg
     4381 acatagcaca aaaaaacata gataatgtca gtatgaatgt tctgtcaatg ggtatgacag
     4441 gagattacac agtagcaata gaagagggcg caacaatggt acgtgtcggt accggaatat
     4501 ttggagagcg cgactacagc aaggctaact gatactttgc atatgctata cacaaaataa
     4561 aaatagaaaa ggaatataga aatgagtttc atagataaga ttcttgacaa aatgagttta
     4621 aattctgagg acgatgagga gttcgataac gaggattatt acctcgatga tgaggaagaa
     4681 gaagagcttc caagaaatcc tttccgcaga aaaaaagagg ctgctgaaga ggaaccggcc
     4741 ataaaaagca caaggcgtga tacaacacca aaggagaagc ctgttaagac tacatccaag
     4801 attacaccaa tatcaaaatc atccagaaag caggtatcat cagacatgga agtttgcgta
     4861 ataaaaccaa gttcaattga agacgaaata gaaataacag acacattact taacggacgt
     4921 acagtcgtca tcaacatgga gggacttaat gtcgatgtgg cacagcgcat catagatttc
     4981 acatcaggct cagcatatgc cctgcacggt aatctgcaga agatatccaa ctttatcttc
     5041 atcgccacac cacacggagt agatatctca ggagatatcc agagtctcat ggattcattt
     5101 gatctcccgg gttcatctta ttaagcggag tctctaatca gctatgaaga aaaataaaac
     5161 aaaaaaacag atgtgtacag caggcatcat tgcattgatt cttgtagcaa ttgaccagct
     5221 tacaaagtat ctggctgtta taaatctaaa gcaatcgtcc acgggtgtgt ttgttctcat
     5281 tccgggagtt ttcgagttca gatacctgga aaaccagagt gctgcatttg gtgttgatct
     5341 gctttcaata atacagaata tctttcattt tacatattgg tctgagaatc cattggcatt
     5401 tttgcgtgcc aaaatgattt ttttctcagt gattaccatt gcagttgtta tacttttgtg
     5461 tgtatggtat cgcaaaatac ctgtctcaaa acgttttaga ctgctgaatg ttgttgtaat
     5521 actgtttgtt gcaggtgcac tcggcaatct gatagaccgt attgtaaata attatgtggt
     5581 tgatttcttt tattttagcc tgattaattt tccgatattc aatgtagctg atatatacgt
     5641 cactgtagcg gcatttatgt ttatcatact cgggctgttt tactacaagg aggaggattt
     5701 ttccttaatc ttccctgaca aaaagcatga tggaaagcat gaaaattagt gcggtatgca
     5761 ctttttaaag tgtgcaatat agttttaaat agttactaaa ataaaaaaga gaaagcttca
     5821 ctatgaatag agatacattt gaagtccaga gtgaacagga gggagaacga ctggataaat
     5881 acttaagcag catttatccg gatatttctc gctccttttt tcaacggata ctaaaagaaa
     5941 atcaaatact ggtcaatgat aagccccaaa aggcaaatta caggctaaag actgatgaca
     6001 tagtagatgt cacaattcct gatgccgtac agacacctat cctgccacag gacattcctc
     6061 ttgacatact ctatgaggat gatgacgtac tggtagtcaa caagccaaaa ggcatggttg
     6121 tacacccctc agcagggcat tacagcgata ctcttgtaaa tgccataatg taccactgca
     6181 aggactcttt aagtggcata aacggcgaaa tacggcccgg tatagtgcat cgcatagaca
     6241 tggacaccac cggctccctt atagtctgca agaacgatga aagccatata aagatatcgg
     6301 aacagattaa ggaccatagc tgcaaccgta tatacgtggg cattgtctgt ggcaatgtaa
     6361 aggatgatga gggcaccata gagggcgcca taggcaggaa tcccaatgac cgcaagaaaa
     6421 tggcgatcaa tgagaaaaac ggcaagccgg ctgttacgca ttataaggtg cttgaacgct
     6481 ttggtgacta cacatatatg cagtttaagc ttgagaccgg cagaacacac cagatacgcg
     6541 tgcatatggc aagtattaat catcccttgc ttggagatat gctttattcg agctattcac
     6601 ctaccaaaaa ccggtttaaa gaccttgaag gacagtgcct gcatgctaaa actatagggt
     6661 ttgttcatcc aaaaaccggc gaatacatgg aatttgatgc acctttacca aaatactttg
     6721 ccaatttgct tgaagtgtta tataatataa ataacaaata aattgaatta tcacatagtg
     6781 atatagtatt aattatttat ataaggaggt ctgaattatg ggatcaaacg aaatttacac
     6841 aataggaaga gaatttggaa gcctcggaaa acaggtcgga caagatttag ctaaaagact
     6901 tggaattaaa ttttatgata aagagctctt acagaaggca gctaaggaat caggactttg
     6961 cgaggaaata tttgagtcgc atgacgaaaa gcctacaaac agctttttat attcgctggt
     7021 catggacacc tacacaggtg gctcttattc cacagcaccg tttcttgata tgcctctgaa
     7081 tcacaaggta tttttagcac agtttgatgc tatcaaaaag attgcagcaa atgaatcctg
     7141 tgttattgta ggccgctgtg cagattatgc tttagcagac gagccgcatt gtctcaatat
     7201 cttcatacgt tccaacatgg acgacagaat taaaagaata agtgaaagac tcaatgtccc
     7261 ggagaacaag gctaaggaca taattaagaa aaaagataaa gagcgctcaa gctattacaa
     7321 ttactacacc agtaaaaaat ggggagacgc aagaagctat gatttgtgcc tgaacacaag
     7381 ccagatatca gttgaggact caatcgagct tatacttaaa tacagggaat gcatgagaaa
     7441 tcacaataaa taattgcgta ataagtaatt gtattgttgt atagaaaaac tatagatata
     7501 aagccatcgg agctgataca aatagaggtt tcggtggctt attcttgtat tattataaaa
     7561 ggtatcatcg atgcatgctt cgatgatacc ttttgaccct ttaatcataa tattgttgta
     7621 cgtatcaaaa tttcaggtat tatgaaaaaa ataagcaggt atatgttttt gttgtagtac
     7681 catagtactt ttacaaggtc atttattcgt taaacttaga tttagcgaat aaatgtgtgc
     7741 cgcaaaaaaa tagtaccgct ccgcttgcag tacatacctc gtcctttaat acactgcttc
     7801 ctttgtcaat gtctgttaat gactggaatt ttctacatct gttatttttc agatatttta
     7861 tattattaaa cacaaaaaga cggttgaaaa attatccgtc ttatattata atcgagataa
     7921 agaaagagtt gatcggatat aaaacttccg attgcctcag tagataaaaa tcttaattaa
     7981 gaaatagcac tactttggtc ggtgggtgct attttctttt attgtgtcca tctatgtatg
     8041 ccagtgcagt aaagattaca agcaacaaag ttaatacttc cattgtgttc atgattacca
     8101 cgcctcctct ccatccggac ataaacagca tgtccttctg tactaggggc aaccgaaaaa
     8161 tcacacccga ctctttccct attcgattat gaaggtatta taacagattg tgaagctcat
     8221 ctcaattaca agctttgaaa ttttataaaa agacggttga aaaatcagcc gtcttttata
     8281 tttgaaattt atgattttat tataaaatag ataacacatc ctccacaatc tgctctgcag
     8341 tcagatgatt ctccttcagt acagcatcca catcatacct gtcaagaaat tccttcttca
     8401 atccataatt catcactttc atattgcttg caccatagaa tcgtgcgatt ttttcaccaa
     8461 agccgccatc taaaatacca tcctctaaag taataacagt atcatgattt tgcttaagct
     8521 tagtcaaaag cgcctcatcc aatccggtga tgtaatacgg attgattaca gttgcgttga
     8581 ttccggcttc ttctttaaga agcttcgcag cctcctttgc cagttcaaag aaggttccca
     8641 gaccgataat cgcaatcttt tctcctgtct gaaccacctc atatctgttg agtctgccaa
     8701 aatcctttgt cacccttgaa ccatctgaaa tcatcggtcc gcccggcagc tttattgcaa
     8761 ccggatagga tgtctgctcc atactccagt cgagcatggc cagatactct tcctttgttg
     8821 ccggtgccag atataccaga tttggaatat ttgaaagcat cggaatatcc tgaaagccta
     8881 agtgtgtaac atcattcatt ccatatacgg aaccctgata tattaccatt gtaatcggac
     8941 tgttattgat gcagacatcc tgtgatacct ggtcaaaggt tctctgtaca aacgaactgt
     9001 atacaccata aaatggttta ccaccattta ctgcgacacc tgatgccagt gccactgcag
     9061 tctcctctgc aatacctaca tcgacaaact gactgccagc ttcttttctc ttatcctctg
     9121 taaagcccat aacagtcggc gtaccggaag taattgcaac aacagaagaa tcatttttca
     9181 tcttttcaag cagatattca caggtcacag aagaataatc ctcttccaca ttgtcaaata
     9241 gcggctttcc tgtttctata tcgaatggac cgctgtaatg ccactgctcc ttgcattttt
     9301 ctgcaggggc ataacccttg cccttaagtg tgtttatatg cactacaacc ggtttagtgc
     9361 tgtccttgac ctctttaaat gccttaataa gagctgcaac atcgttgccc ctgtccacat
     9421 acacatagtc aagtcccata gccttaaaca gattgcattc tgtctgaccg tcagtctttc
     9481 gaagcagagc aagattctga tagagacctc catgattttc agcaatcgac atatcgttgt
     9541 catttactat aataataaaa ttgctttgaa gctcagcagc aaaatcaagt ccctcaagtg
     9601 cttctcctcc gcttaatgag ccgtctccga tgacagcaat cacattgccg ttttcaccct
     9661 ttaagtccct tgcctttgca agacctgcag ccagactgac cgacgttgat gtatgtccga
     9721 ctgtaaaaaa atcatgctcg ctctcgtttg gatttgtata gcctgacaca tcatcatagt
     9781 gctgttcaga caaatacgct tccttacgcc ccgtaagcat cttatgagga taactctggt
     9841 gcgatacatc ataaacaatc ttgtccttag gtgattcaaa tacataatgt aaagcgattg
     9901 tcgcctctac cattccgaaa ttcggtccaa aatgtccgcc atgcttactt gctcttacca
     9961 aaagnnnnnt ggtccgttaa ttttctcaag atacatatta tattttgtcc tcctatttgt
    10021 cccaggtcaa ttcacctgtc tttatcgcat tctcgtattt agaaatttta tactgtaagc
    10081 gttccatcgt gttttcaatc tgttttttct gctcatcaag ctgctcgtat tgctccttga
    10141 gaagctgaca ccttgcttca aatgtggcat ctcccatctg aaaaagcctg atatattcaa
    10201 taagtgcctc ctttttgatt gttacaatga aactatatca cttggagtcc actccaagtc
    10261 aagagctttt catatgtttt gtatacataa aaaaattatc gtgttataat ctcaataata
    10321 aaatttaaaa agatttacac ttggaggaaa aatatgaact atacagaatg tatcgaaaat
    10381 gccagaccac gtcttggaaa atactgcaag gcctgtcctg aatgcaacgg ccgcgcatgc
    10441 aaaaaccaga tgcccggtcc cggctcaaag ggcatcggag acacagccgt acgcaactac
    10501 gacaaatgga aacaaatccg tgtaaacatg gatactattg ccgaaaataa gcctgtagac
    10561 acctctctct cactcttcgg ccgtactttt aaatatccgg tatttgcagg tcctgtaggt
    10621 gcagtacagc tgcactacgg agactgtctg aatgatgtaa catacaacga tatccttgtg
    10681 tcagcctgtg caaagaacgg tatcgcagct ttcacaggcg acggaactga cccaaatgtc
    10741 atggttgcag caaccaatgc tatcaaaaat gcagatggtg caggaatacc taccgtaaag
    10801 ccatggaaca ttgagaccat acgtgaaaaa atggagctgg tacatgaatc aggcgcattt
    10861 gcagtagcta tggatatcga tgcagccggt cttccattcc tcaaaaatct tgatccgcca
    10921 gccggaagca aaacagtttc agagctttgc gatatcatac agatggccgg aaccccattt
    10981 attgtaaagg gaattatgac cgtaaagggt gcgcttaaag caaaagaggc aggcgcaagc
    11041 gctattattg tatcaaatca cggtggacgt gtgctggatc agtgtcctgc cactgctgag
    11101 gtgcttgaaa gcatcgtaaa ggctcttgaa ggctcaggca ttaagattct tgtagatggt
    11161 ggcatcagaa gcggcacaga cgtctttaaa gcccttgcac tgggtgcaga cggagttctt
    11221 atcgcaagac cgtttgtaac ggctgtatac ggcggtaagg ccgatggtgt acgtgcatac
    11281 atagacaaaa tcggtacaga gcttgaagac accatgaaga tgtgtggtgt atcttcgctt
    11341 gatgaaatca caagggactg tgtgatgacc ttctagttta aaaacgtgct tcatgacttt
    11401 tcgtttttat ataaattctt catacaaaag caccataacc ggcatcgtca ctacagcaca
    11461 aagcgttgtc actacattta ttgcgctggc ataccttgaa tcattgccat acacctgaca
    11521 catctgtgtg acagtcgatg ccgaaggcgt aataaccgct aagaaaacaa taagcataat
    11581 ctttggcgca tctgcagaaa gatatgccat gtggctgatt ttaataagaa taagcgatat
    11641 caacggaacc agaagcatcc tgagaaaggt aacaaatatg gaaaagcctg cccatagcag
    11701 aaatgataaa cagcaaaact aactgcaaaa tcaccgatgc cacaaatgca atcaaaagac
    11761 ctctcactgt ttttgaagtg taatccacct gaaaagcatt gataataaca cacggtataa
    11821 ccagatacag cacaatcttt gataaaacct tgctgtcctc atccttcaca atacctgctt
    11881 tcacaataat aaaacccatg aaaatcatga taaaaagctc tattatctgt tccattaaaa
    11941 gtatactgat atgcataaaa atcatctcct gctctaaata tctatgtctt aaaaggcatg
    12001 catatctaat tcataattta taaatctata atctcgtagt ccactttgaa caatcccaca
    12061 catccgtcgc aagcccatca tagaaatccg gctcgtggca taccatcagt atgcttccct
    12121 tgtactcctt taaggcacgc ttcagctcat ccttagcatc cacatccaga tgattggtag
    12181 gctcatccag aagtagaata tttgtctctc tgtttatcag cttgcacagg cgtacctttg
    12241 cctgctcacc accgcttaac acacgcacct ggctttcaat atgctttgtg gtcagaccac
    12301 acttggcaag cgcgcttcgc acctcgtact gcgaaaatgc aggaaactcg ttccatatct
    12361 cctcaataca tgtggttgta accgactgat ccatctcctg ctcaaagtac cctatctcca
    12421 gataatctcc aagtgtaaca cttcctgaaa gcggctttat cagcccaaga atactcttaa
    12481 gcaatgtcgt ctttccgatg ccgtttgcac cggtaagaac aatcttttgt cctctttcca
    12541 tctcaaggtc gagagcagat gaaagcggat catcatagcc aatgaccagt ccatttgtct
    12601 ggaaaatata ccgccccgga gtacgcgctg tcttaaagtt aaactccggc tttggctttt
    12661 cagcagcaag ctcaataaca tccatcttgt caagcttctt ctgacgtgac atcgccatat
    12721 tccttgtagc tacacgcgcc ttatttctcg ccacaaaatc cttcagctct ttaatctcct
    12781 gctgctgttt gttgtacgca gcctcaagct gggctttctt catctcataa acttccataa
    12841 atttgtcata atccccgaca tacctgtcga gcttcttgtt gtccatgtga tagatgagat
    12901 ttaccacgct gttcagaaac ggtatgtcgt gagaaataag aataaacgca ttctcataat
    12961 cattaagata tcgcttaagc cactcaatat gctctacgtc cagatagttt gtaggctcat
    13021 ccaaaagcag gatatccggc ttctccaaga ggagctttcc cataagcact ttggttctct
    13081 ggccaccgct taaatcggtg acatccttat caagccccag gtctaaaagt ccaagagccc
    13141 ttgcaacctc ctcaaccttt gcatcgatca tatagaaatc atgcatggta agcatgtcct
    13201 gaatcgtgcc ggcttcctcc atcagcttat tcatctcgtc ctcgtccaca tcgccaagac
    13261 gcgcatatat ttcgttcatc tcattcctgt gtaataaatt ccccatattt ttcataagaa
    13321 taaatttcct ccagagacat ttcctttaat gaatccttaa tatctccgga tattttattt
    13381 atatcatcat ttgaaagatt ttttacaatt ctcctataca ccaatgcatt actaaaaaat
    13441 atttctgcat tatcattaac tattctatga caagaagaag aagatatatc atcagcagaa
    13501 aaacaattag ccctgttttt aaaatagtcc ttgaatgatg ttgcaaatct ggaaaataat
    13561 ttaattacct gtgttttttc ttccttttct gatgcagaat aattattgtt atgaatgaca
    13621 aattcaggaa gaatatctga gattaatttt gcactgaaca tatttttaaa tctatcatca
    13681 tctgcaaatt ttttatagat tgccttacgt ttttcagcct gttcttttat aagagtgtct
    13741 ttattatctc cattttttaa ctgaatttcc attttctcaa ataaagaggt ccagtcaata
    13801 tcatcaattg acgataaagt ttctgaaatg aaacctctgt aataatcatc catgatatct
    13861 ttaagtatct gacgattttc tcctctgagt tcatcttctt taattattcc atttttaaca
    13921 ataaattgct gtgttgtttc tgttggaatg agagcattcc taagagtctt ctgcaaagaa
    13981 gaaattccga taaagttctg aaagttattt gttccgttat tcatatttct ctcctttttt
    14041 ataaagatat gtaatgtgta actagcgtcc ttgtaaaaaa ataacgtttt ttaacgttac
    14101 ttctttaata cctgatatct ttaatctaca aataagtaga aattacttgt aattcaaata
    14161 tactattttc ctatggcgat gtcaaatatt ttgcttaaag aagtaacaat gggtcttcgt
    14221 tttttgctat gtttgttttc ttatcttctt actttcttgt gttgacccta gcttatcaca
    14281 ttacgagtcc caaagtctgc cctcgtaact cgaatttttg ttcgaagatt actgttcagt
    14341 ccctagccag aagccctctc tcgtaaccat attttattta taaaaacatc tataatcctt
    14401 aaacaaaaaa tcgctcaaac tcacggtcta agcgattttt ctattcggga attggtttag
    14461 tttcccatct ttatataagc tctactcaag cttgaatttg ccgacttccg ttgccagatc
    14521 ctctgcgatg ccattgttgt cggaagcctc gctttggata tcattgacgt taccggtaag
    14581 ctgtacggaa gactcggaaa ctcccgcaat gcccttggca ctctcttcca cggcaatgtt
    14641 gactgcagag atggattcac ggatgccatc catcgtgctg cgaagttctt ccgcagtctg
    14701 cgcgaactgc tccatcattg cacgcattgc attcgcatcg gtcgcataat tttcactcgt
    14761 tgcaacaaga cctccgaagc cttcctctgt ggaattgcgg acaaattcga tcatctgctg
    14821 cgatccctct gccagttcat ccactgcctg aatgacctct gcactcacct ggcggatttt
    14881 tgctgcggca tccgcagaat tacctgccag ctttccgatc tcatccgcaa ccaccgcaaa
    14941 gccgcgtcct gcttcccctg cgcgtgccgc ttcaatactt gcattcagcg caagaagatt
    15001 ggtctgttcc gtaatctcga taatgttgga agtcagaatc tcgatctgtt ccaccgattt
    15061 ggactttgca atcttctcat ttaaagaagc cgccatcttc tcggtctcaa tgtttgcttt
    15121 cttctctccc tccgctgctg catcattcgc cccggaagca cggctctcca tctcctgaga
    15181 agagactttt ccggcatccg cagagcctgc aatacgctct accgatgaat agatttcgtc
    15241 aattgcctcc gtgatctgat tgagtgaaga agtcgtttcc tccatcgccg cactcatctc
    15301 ctgcatcgtt gccgacacat cggtaatgct ttcatccgca gagctcagat gatccaccat
    15361 tttgcgggaa gaagacataa gccgcatgga tccggacgaa attccgatca taatcttgcg
    15421 gatatacgct aacaactcat tcacattgcc cgcaatcagt tccagctcgt ccccgcttct
    15481 gacatccagt ttctgagtca gatcaccctc attatgtacc agatcataaa tcttcgcatt
    15541 tacctgcatc agtccctttg taatcctgct aacgataatc gtcagaatca ggattgccag
    15601 aatcagacag atgcctccga tctgtaacgt ctggacaact gccttctgta attctgctgt
    15661 aatcgatgac gcatcataat cgcatccaag tattgcaaca accttgcctg cattgtcctc
    15721 aataggcttg tataccgtaa tcagatctcc gtcttctgta tggtcgatgt aatcctgaat
    15781 gtattccttt ccaccgaaca ccggctccag ttccgcgtat gaatccgcaa attcatcacc
    15841 gacttttgcc gcatcctcgt ccgaatccac accataatac actttctttc cgtccgtata
    15901 caatgtgtat aaaaaagcaa ttccgcatgc tttttgcttt tttctgagat ctccttgcag
    15961 attctgatag acctgcgtac cttccgatcc taccgtcact ttgtatacca gcttggcatc
    16021 cagcgaatcc gcaacgatcg ttgccgccat gtctgcttcc tcaacgccca tcctcaccat
    16081 gcttttctca aaacgcgtgt aggaattgta tcccatcaaa ccacagacga gtacaacgat
    16141 tatgattgac ggaatcagga tcttcgcgcg gattgtgagt ctccttgtac gaattttgcc
    16201 tttgttcatt tcaacgcttc tccttatgtc tacattgtat ggcacaagta taacacatta
    16261 tctaacgatg ggcaacgggc atgttcgtca attattatcg ccttacaaac tgggactttc
    16321 atttacaaac caggaatctc ggttacaaaa caggactttc atttacaaat ggggagtctt
    16381 gggttacaaa tgggattctc agtttactat ttaccaaaac ataataatct ttttcacata
    16441 acgttatata tttattattt tttgtaatat atactttaca tttagttcga tatatcatat
    16501 aatgcatata gaaaggagga ggttgtataa atgaaaaata tgcgtcttaa attagcacgc
    16561 gttgagatga atctttctca
//
LOCUS       DPUF01000164           18989 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16433, whole
            genome shotgun sequence.
ACCESSION   DPUF01000164 DPUF01000000
VERSION     DPUF01000164.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 18989)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18989)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18989
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16433"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="DHV81_05780"
     CDS             complement(<1..503)
                     /locus_tag="DHV81_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93983.1"
                     /translation="MKKDSFKLIIMSVAVICIVIAGIMVPGIVLEANQKSEHDHVAKV
                     PKSYLPSGSVMAKEASANLKLSEKLSLIDGQWDSTTDDAASYEMDEQDYQAVATCRKS
                     IEKLYDEKLYPVNLSPDYSNWYTWEAAAKKASDNTFNTYTAYYWIIRFEKYDGTQNHT
                     IWMLEDGT"
     gene            complement(559..2106)
                     /locus_tag="DHV81_05785"
     CDS             complement(559..2106)
                     /locus_tag="DHV81_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI93984.1"
                     /translation="MKFRYKVLFTNLILLSLGLGLVGYLMIHKNFELAKQTQLKNAIV
                     QNNLVQSSVEYELLQLLNSVSDNSETSNNNTDNNNAEKSSISASSIVAQLPQIGSRVS
                     SSVRSRDSFFYIYFDGEKVYTDDKSDARISDTLFKNLTTGNKNYVIKEESQKHYIYVT
                     SQSVINEKNLWIVSKCDASEAYTLLDEQINYFRLIIIVILIAESAAMYFISRYMTKPL
                     EKLNHVSEEIAQGDYATRVNVKSHDEIGLLAQKFNIMAQAVSDHVDELNDMVHRREQF
                     VADFTHEIKTPMTSIIGYADTMRSLELPREEQMMALGYIFSEGKRLESMSQKLFELIY
                     LRRHDIEKARVHMADLCAEVVKVVEPAYENRHLTIETEIEDTVLTVNRELLVTALVNL
                     MDNARKASEEGQQVEVTGKFVDNMACNIPKDKTDIGEAESADDRKCTRAYEICIIDHG
                     IGMTKEQAERICDEFYMADKSRARKEGGAGIGMSLVAIILEHHNAVLSVESEPGCGTT
                     MRILLPW"
     gene            complement(2160..2813)
                     /locus_tag="DHV81_05790"
     CDS             complement(2160..2813)
                     /locus_tag="DHV81_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI93985.1"
                     /translation="MIEILIVEDEKPISDLIRLNLMKAGYSCTCAFDGMEAADILEER
                     NFDLIILDVMLPKLNGFELMEYIKPLGIPVIFLTAKATLDDRLRGLTSGAEDYMVKPF
                     EIVELLARVNIVLRRYNKMEQQLTFKDIAIDTKNQNVSKAGKHIELTPKEFELLELLM
                     RNKNITLFREKIYEEIWGSEYSVESRTLDLHIQRLRKKLDIGDSLKTVFKAGYRLEE"
     gene            complement(3050..4102)
                     /locus_tag="DHV81_05795"
     CDS             complement(3050..4102)
                     /locus_tag="DHV81_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCI93986.1"
                     /translation="MEQRFITMGEIMLRLTPPNYEKIRMTHSFEASYGGAEANIALAL
                     ANLGIDSTFFTVVPDNSIGKSAIRMLRSNDVHCAPIILSTPDETPTHRLGSYYLEAGF
                     GIRPSKVIYDRKHSAITEYDFSKVDLKKLLTGFTWLHLSGITPALAPNCRQLIMDTLK
                     VAKALGITVSFDGNFRSTLWSWEEARDFCTQCLPYIDVLIGIEPYHLYKDPSNHSLGD
                     VKDGIPLHLSYEQEDEIFSNFAIQYPNIKYMARHVRYAHSGSENSLKAYLWYNGRTYE
                     SKMFTFTILDRVGGGDAFASGLIYAMMNNYEPMDIVNFAVASSAIKHTIRGDANITDD
                     VESIKNLMNMNYDIKR"
     gene            complement(4131..5228)
                     /locus_tag="DHV81_05800"
     CDS             complement(4131..5228)
                     /locus_tag="DHV81_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCI93987.1"
                     /translation="MNLNLKPKSECKYDAVSLGEVMLRLDPGEGRIRTARSFRAWEGG
                     GEYNVVRGLRKCFKMNTGVITAFADNEVGKLMEDFICQGGVDTSLIKWMKTDGIGRVC
                     RNGLNFTERGFGIRGAVGCSDRANTAISKATPEDFDFDYIFGELGVRWLHTGGIYAAI
                     SEQTCETVLAAIKTAKKYGTIVSYDLNYRPSMWSAIGGQAKAQEVNKEIAKYVDVMIG
                     NEEDFTACLGFEIEGNDANLKELNLDGYKKMINEAAKAYPNFKAVATTLRTVKTATVN
                     DWSAICWADGEVYKAKDYNGLEIMDRVGGGDSFASGLIYGLMTTEDAETAVNYGAAHG
                     ALAMTTPGDTTMVSVNEVEAIMGGAGARVQR"
     gene            complement(5245..5883)
                     /locus_tag="DHV81_05805"
     CDS             complement(5245..5883)
                     /locus_tag="DHV81_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HCI93988.1"
                     /translation="MNTIIEKLKEMLVVPVVVLDDVKDAEKLADALVGGGLPCAEVTF
                     RTAAAEESIRIMTEKYPDMLVGAGTVLTTEQVDKAVAAGAKFIVSPGFDAEIVDYCLD
                     KNIPILPGVISPSEAAQAVKRGLSVVKFFPAEQYGGVTTIKALAAPYTMLKFMPTGGV
                     SVKNLKDYLSCDKIICCGGSWMVKGDMIKSGEFDKIKQMTKEAVELAHSIRK"
     gene            complement(5927..7711)
                     /locus_tag="DHV81_05810"
     CDS             complement(5927..7711)
                     /locus_tag="DHV81_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCI93989.1"
                     /translation="MLRSEEVRKLAPEMDPLRMGMGWKVSDLSKPQIMIESTYGDSHP
                     GSAHLNKFVEEAVKAVDENGGKAARYYATDMCDGIAQGHDGINYSLAHRDAITNLVEA
                     QANATVFDGGVFIASCDKAMPAMLMSIGRLKDMSAIMVTGGVMEAHELPKKYIVDDPA
                     CKINELLTLEQIGKFDAWEKTGVIPNEQLDYYKHNACPSCGACSFMGTASTMQIMAEA
                     LGLMLPGTALMPSTAPELKEASYNAGKQLMELIAKGITAKDIVTKESFENAIMVHAAI
                     SGSTNATMHLPAIAHEFGIRIDADTFDRLHRGAHYLLNIRPSGDWPAQYFYYAGGVPR
                     VMEEIKSMLHLDVMTVTGKTLGENLEELKKNGFYEHCDEILREKSKIFGRAIDREDII
                     HSFDNAKGTDGSIAILKGNLAPEGCVIKHTACPKEMFKAVLNAKPYDCEEACISAVLN
                     GEVKPGDAVFIRYEGPRGSGMPEMFYTGEAICADPKLASSVALITDGRFSGASRGPVI
                     GHVSPEAAAGGPIAFVEEGDLIELDVENRKIAIVGIKGERKTPKEIDAVLADRKAHWK
                     GFKSKYQHGLLKLYAQHAVSSMKGAYMD"
     gene            complement(7728..9083)
                     /locus_tag="DHV81_05815"
     CDS             complement(7728..9083)
                     /locus_tag="DHV81_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCI93990.1"
                     /translation="MSEAATLDPTRLVIAAIIGLALLLVLIIKFKVHAMISILLGAIT
                     IGVVAGMPLTEIVTAVNDGISNTLKGIALLVGLGSMFGAILEASGGAQTLAVTMVKKF
                     GDEKAAWALGVTGLIIAMPVFFDAGLIILIPLAFSLAKRTKRSTLFYAIPLLAGLAVG
                     HAFIPPTPGPVLVATMLNVDLGWVILVGIACGTVAMIIAGPVWGSICGKKYMVPVPEH
                     VASQEDIDESKLPKFSTIVLIIAIPLVLIIFKSLAGVIPAMAPVAPVFTFLGEPFVAL
                     LIATIVAMLVLGKRHGYSMEELEKIMTKSLEPTGLILLVTACGGVLRYVLQYSGLGDL
                     IGNAVASANLPIVLVAFIVAALVRICVGSATVAMTMAAGIIAAMPGISELPPLYLACV
                     VAAVAGGSTVCSHFNDSGFWLVRSLVGVDEKTNLKTWTIMETLVGFSGFVVAFIISLF
                     V"
     gene            complement(9301..10020)
                     /locus_tag="DHV81_05820"
     CDS             complement(9301..10020)
                     /locus_tag="DHV81_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCI93991.1"
                     /translation="MPYNYEKVNQKLLVPQIEDQLVMYIQHENIGIGAKLPSEYKLAE
                     IFGVGRSTIREAVKSLVTKGMLEVKRGSGTFVKSTEIVVDDPLGLAQFEDKYRLAMEL
                     FEVRMLLEPDVAAVACQKATEEDKKRIKSLCDEVEQLYLSGQNHIKKDMEFHETIARC
                     SGNRVVEVLIPIILTAITTFANLTHRKLINETIDTHRAITDAILSGDTMGAKCAMIMH
                     LTYNRQTIIDMSEQNKTCQTS"
     gene            10177..10980
                     /locus_tag="DHV81_05825"
     CDS             10177..10980
                     /locus_tag="DHV81_05825"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01380.20,HMM:PF01418.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93992.1"
                     /translation="MLILKLNLNIPLQSLSTKELDILQYVHDHSDNISSISIQQFANE
                     LNYSTSTILRFCRKLGFSGFSEFKYFLKSSDNEDNNKIKGSDKSLDIIKNTIKDSMDS
                     TVSLLNSDDMFQIARLFSENRPIYLHSPAGLTDIAVSYLESMLFLSGCQKIYKTTAAK
                     AAHHFIQTVDKGNVFVFISNSGRFEPTLNLAKEARLHGMIVISISSIENNDLAEVSDY
                     NLRFFSKPREYEGADFTSRLGSFFVISSFMEYFNLQRKGDNSNEPSASD"
     gene            10958..11689
                     /locus_tag="DHV81_05830"
     CDS             10958..11689
                     /locus_tag="DHV81_05830"
                     /inference="COORDINATES: protein motif:HMM:PF01418.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI93993.1"
                     /translation="MNLLHQIDTTDFSENELLILEYFIAHEKSLPYMSLNDICKDLFI
                     SNATIVRFCQKIGFNGFNELKYSLRKASRESTGFSTWSVMQHHTSILKDFIDGLDSAQ
                     LETICKEVTQSDSLYIYGRGLSSLPARYLYSMFNAIDIPCIYIDWIDFLLAISVSFPE
                     NATLILFTNYGEKEIYEPIAKRCHTKNVKIIWISSVDIDSSLLAASDICITAPELPSE
                     NTHLRTKMTAFVFVQIIIEYILTQK"
     gene            complement(11776..13812)
                     /locus_tag="DHV81_05835"
     CDS             complement(11776..13812)
                     /locus_tag="DHV81_05835"
                     /inference="COORDINATES: protein motif:HMM:PF00358.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCI93994.1"
                     /translation="MKSKIMKFFSDLGRSLMMPVAALAACGIILGITSALLKTQVQDA
                     VPFLTASVPLFIITTLNKVSGIVFTLLPVLFAISIAFGLAKEDKGVAAFAGFIGYYTF
                     LVASSCMISTGIMDFSAMKISNILGVDTVDMGALAGIMAGLVTAKLHNKYHKVQFPAA
                     IAFYGGKRFVALVVLVSMTVIGLVMPLIWAPISVGIDGLGRIIRSSGAFGVFIFGTLE
                     RLLIPTGLHHVLNSLFRTTAIGGTYHGVEGCLNIFLQFLDQAPLSDMKPYTQFLGQGK
                     MPYMLFGLPAAALAIYRTTPQDKKAKVKALMIAGVAACFVSGITEPLEFSFMFIAPAL
                     FVFHAIMGGISFMLMSLLGVIVGNTGGGFIDFLLWGVFQPGSHWYWVIVVGIPYAFIY
                     YFVFKWYLSRKKIVVSVSDDDDSDAKSDEKTNEGKKSGSNKTALQVIAGLGGMANIVV
                     VNNCISRLRVDVKDMSLINEETLKKTGSLGIVKTSDTHIQVVYGPKVEKIADAVRAAL
                     NDKDCAAEVEKVQDEKPKSTVIISSPMTGLAENLSKAPDEAFANKLMGDGAVVTPKDG
                     TVVAPDDGTVLFVFDKKHAIGFATDSGVSMIIHVGIDTVKLDGKGFDVKVETGAHVNK
                     GDVLMQLDLDFLRENAPSIASPVICTELKENQSIRLINEGEIKAGEPLFAIDTY"
     gene            complement(13855..15180)
                     /locus_tag="DHV81_05840"
     CDS             complement(13855..15180)
                     /locus_tag="DHV81_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001252810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HCI93995.1"
                     /translation="MKKYNVCIVGGGSTYTLGFLKSFARMQEEFPLNKLVLFDIDGER
                     QKPIGQYGDIMFAERYPELDFSYTTDPKEAYQDMDFIFMQMRAGGLHMRQKDEHIPLS
                     FGKVGQETCGAGGMAYGLRSCVDMIEAVHQIREYSPEAWILNYSNPAAIVAEALRREF
                     PDDKRILNICDQPENVVRSTSRLLGCDWGDLDPVYFGLNHYGWFTHMYDRKTGEDLLP
                     KIRQIVKEKGFLPQDAEQRDQSWLDTYGFVQTMMEDFPDYLPNTYDGYYLYPEYKTSH
                     LNPEYTRADEVIAGRESRVFKEAAEVIKAGKLGDKFHAISDAHAEMMIKVAEAIAFNT
                     LGRFILIVENNGAIANMQDDAMVEVVCELGINGPRPMSVGNIPQFYLGLLVQQVSCEK
                     LLVDAYYEHSYQKALEAFTLNRLINDAKVARKILDALIEANKGMWPELK"
     gene            complement(15419..16450)
                     /locus_tag="DHV81_05845"
     CDS             complement(15419..16450)
                     /locus_tag="DHV81_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Archaeal ATPase"
                     /protein_id="HCI93996.1"
                     /translation="MKNNPFSLSFGKEPVSLINRNVQSYEIIDTFEDENPTYQVCMIT
                     GVRGSGKTVMLTEINKTFRAEEDWIVIDLSPERDLLQSFAANLSNYPSLSEVFREAKI
                     NLSFLGLGVEIEGVSPITDLNVAVTRMLEKIKKKRKRVLVTIDEAVCNDTMKEFVSLF
                     QIYMRQDLPVFLLMTGLYENIYELQNEKTLTFLYRAPKIELRPLNIGMIAEKYKSIFE
                     LTDEEATEMAKETRGYPFAFQVLGYLCFKHNTGWHNVLPEFSQYLEEYVYEKIWSELS
                     KGDKELLVAMAKTNDTKVEAIRKTIKKASNSFSVYRNRLIKKGLIISPEYGHLEFTLP
                     RFKEFVLCR"
     gene            complement(16591..18444)
                     /locus_tag="DHV81_05850"
     CDS             complement(16591..18444)
                     /locus_tag="DHV81_05850"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516824.1"
                     /note="class 3; catalyzes the formation of fructose
                     6-phosphate from fructose-1,6-bisphosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCI93997.1"
                     /translation="MYNDKGNTKYLKLLKQNYSSSQDVIREIVNLSAIINLPKGTEFF
                     LSDIHGEYEAFLHIMNNCSGVIKEKVDLIFKDTISDYDRQELCTLIYYPREKMALLDE
                     QGKIDSDWYAMTLNQLILVAKLLSSKYTRSKVRKALPEEYAYIIDELLHAQEDEDANQ
                     VRYHKQILKTIIDLEDADEFIIALSALIKRLAVDHLHIVGDVFDRGGSADKILDLLYD
                     YHSLDIEWGNHDILWMGAACGNDACICNVIRNNLKYNNVEILENAYGISLRQLMLFGM
                     KTYGIEDGIEAARAAITVMLFKLEGQLILAHPEYEMGNRLMLDRLDELQDVGVDRKRF
                     PTVDDERPYALSDEETVIMRKLHRQFVTGQRLHKHVRFLYDKGGMYNVYNGNLLYHGC
                     VPVDSNGAFDKLYIDGEFYHGRALLDKCDEKARAAYVDNPYKDDVDFMWFLWGGEKSP
                     LCGRRLKTFEMEYVTDKSMWEEPSNPYYSRYYDKSFCCQILHEFGLYDDDAHIINGHT
                     PVHAIEGENPVRANGKLFVIDGGFCRAMNKKTGIAGYTLIYNSHGLRLKAHTPFTSVE
                     DALINNTDIETSSEVEENDKRRMLVKDTDIGKRLIGEIDDLHKLLENYRRL"
     gene            complement(18663..>18989)
                     /locus_tag="DHV81_05855"
     CDS             complement(18663..>18989)
                     /locus_tag="DHV81_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCI93998.1"
                     /translation="SLAEVGVDSIPLNALMPIAGTPLEGLEHISEPDILRTVSFFRYI
                     NPKADIRLGAGRALLTNDGELAFKSGASATITGNMLTTVACATIRSDKEMLEGMGRNV
                     TPEYMK"
BASE COUNT         5022 a         4686 c         3313 g         5968 t
ORIGIN      
        1 gtcccatcct caagcatcca gattgtatga ttttgtgtac catcatactt ctcaaacctg
       61 attatccagt aatatgccgt atatgtgtta aaagtattat ccgatgcctt tttcgctgct
      121 gcctcccacg tataccagtt agagtagtca ggtgaaagat ttacaggata gagcttttcg
      181 tcataaagct tttctatgct ttttctgcat gtggcaactg cctgataatc ctgctcatcc
      241 atctcataag aggcagcatc atctgtcgtg ctgtcccact gcccatctat gagcgagagc
      301 ttctccgaca atttcagatt ggcagatgct tcctttgcca ttacagagcc tgaaggcaga
      361 tagcttttag gaaccttcgc cacatgatca tgttcacttt tctggtttgc ctcaagcact
      421 atgccaggca ccatgatgcc tgctattact atacagatta ctgcaacaga cattattatt
      481 aatttgaagc tgtctttttt catatcttcc tttaaatctt acgaatttac cattttttat
      541 atctatactg cgttcagatt accacggaag caaaatcctc atcgttgttc cgcatccggg
      601 ttcactttcc actgaaagta ctgcattatg gtgctctaaa atgattgcca caagcgacat
      661 tccgattccg gcaccgccct cttttctggc tctggatttg tctgccatat aaaactcatc
      721 acaaattctt tctgcctgtt ccttagtcat tccgattccg tgatcgatta tgcatatttc
      781 atatgccctc gtgcatttcc tgtcatctgc tgattcggct tcgcctatat ccgttttatc
      841 tttaggtata ttacaagcca tattgtccac aaattttcct gtaacctcaa cctgctgacc
      901 ttcttctgac gctttccttg cattatccat cagattgaca agtgctgtaa ccaaaagctc
      961 tctattcacg gtcagcacag tatcctctat ctctgtttct atagtaagat gcctgttttc
     1021 gtatgcaggc tcaacaacct ttaccacctc tgcacaaaga tctgccatat gaacccgcgc
     1081 tttttcaata tcatgccggc gcagatatat cagctcaaaa agcttctgcg acatgctctc
     1141 cagacgtttt ccctccgaaa agatatatcc aagcgccatc atctgctcct ctctcggaag
     1201 ctccagtgaa cgcatcgtgt ctgcatatcc aatgatcgac gtcatagggg tttttatctc
     1261 gtgtgtaaaa tccgctacaa actgctcacg cctgtgcacc atatcgttaa gctcgtctac
     1321 atgatccgat actgcctgcg ccatgatatt aaatttctgc gcaagaagcc ctatctcatc
     1381 atgacttttt acgtttaccc ttgtggcata atccccctgc gctatttcct cgctgacatg
     1441 gttaagcttc tcgagcggct ttgtcatgta cctgctgata aaatacattg ctgccgactc
     1501 tgcaatcaat atgactatta ttatcagcct gaaatagttt atctgctcat caagcagtgt
     1561 atacgcctct gatgcatcgc atttacttac tatccagaga tttttttcat ttatcacact
     1621 ctgcgatgta acatagatat agtgcttttg ggattcttct tttatcacat agtttttgtt
     1681 tcctgttgtg agatttttaa aaagtgtatc acttatcctt gcatcacttt tgtcatctgt
     1741 atatactttt tcaccatcaa aatatatgta gaaaaatgaa tcccttgagc gcacactgct
     1801 gcttacacgg cttccgatct gcggaagttg cgcgactatt gatgatgcgg aaatgctgct
     1861 cttttctgca ttattgttat ctgtattatt gttcgaagtt tcagaattat cggaaactga
     1921 atttaaaagc tgcaaaagct catactccac agaggactgt accagattat tctgcacaat
     1981 agcattttta agctgtgtct gcttcgccag ctcaaaattc ttgtgtatca tgaggtatcc
     2041 caccagcccc agtccaagtg aaagcagtat taagtttgtg aacaatacct tgtacctgaa
     2101 tttcatttat ccctcttttt gtatattacg cttacccata tttaatggtc tttagttttc
     2161 tattcttcca gcctgtatcc tgccttaaat acggttttaa ggctgtctcc tatatcaagc
     2221 tttttgcgca gtctctgtat gtgaagatcg agagtcctcg actcgacaga gtactcactt
     2281 ccccatattt cttcatatat tttttctctg aaaagtgtga tatttttatt gcgcatcaaa
     2341 agctcaagca gctcaaattc ctttggtgta agctctatat gctttcctgc ctttgataca
     2401 ttctggtttt tcgtatcgat agctatatcc ttaaaggtga gctgctgctc catcttatta
     2461 taacgtctta gcactatatt tacccgggct aaaagctcaa caatctcaaa aggctttacc
     2521 atatagtcct cggcaccgga cgtgagacct cttagcctgt catctaaagt ggcttttgct
     2581 gtgagaaata tcacaggtat gccaagcggc tttatgtatt ccataagctc aaagccgttt
     2641 aacttcggca gcattacatc aagtattatc aggtcaaaat ttctctcctc cagaatgtct
     2701 gctgcctcca taccatcaaa ggcacaggtg cagctatagc ctgctttcat gagatttaat
     2761 ctgatgaggt ctgatatcgg tttttcgtct tcaactatta atatttctat catcattttc
     2821 cttctaaaac tttattatcc gtaactattt tcctttataa atctttgcat attcttcttt
     2881 tgctgtcaat cctaaggatt atatattcag cctgtattat gcatggctat ttatatatta
     2941 acagctactt gtatctaaaa tgtatcagac acaagcaatt acatacgaaa aatccgccat
     3001 gcgcattccc cggcatgacg gattactctt taatcatatt ttagtttatt tatcttttga
     3061 tgtcataatt catattcatg agattcttta tcgattcgac atcatcagtg atatttgcat
     3121 cgcctcttat tgtgtgcttg attgcactgc ttgccactgc aaaattcact atatccattg
     3181 gttcataatt attcatcata gcgtagatga gtcctgatgc aaaagcatct ccaccaccaa
     3241 ccctgtcaag gattgtgaat gtaaacattt tgctctcata agtccttcca ttgtaccaaa
     3301 ggtacgcttt gagactgttt tcgcttccgc tgtgagcata acgaacatga cgagccatgt
     3361 attttatatt aggatattga attgcaaaat ttgaaaaaat ctcgtcttcc tgttcgtagc
     3421 tcagatgaag cggtatccca tccttcacat cacccaatga atgattggat ggatctttat
     3481 acaaatgata aggctcgatt ccgattaaca catcaatgta tggcagacac tgtgtgcaga
     3541 agtctcttgc ctcttcccat gaccataatg tactgcggaa gtttccatcg aagctgaccg
     3601 taatgccaag cgcctttgcc acctttaagg tgtccataat aagctgcctg cagtttggtg
     3661 caagcgccgg tgtgattccg cttagatgca accaggtaaa tcctgtcaac aatttcttca
     3721 agtcaacctt ggaaaaatca tactctgtta tcgcactgtg ctttctgtca tagattacct
     3781 tgcttggtct gattccaaag cctgcctcca aatagtagct tccgagtctg tgtgtcggag
     3841 tctcatccgg tgtcgacaga ataatcggtg cacaatgcac atcgtttgag cgcagcattc
     3901 tgattgcgct ctttccgatg ctgttatccg gtactaccgt aaagaaggta gagtcgatgc
     3961 cgaggtttgc aagcgcaagt gctatattgg cctccgctcc gccgtagctt gcctcaaatg
     4021 aatgtgtcat tctgattttc tcatagttgg gcggagtcag tctaagcatt atttcgccca
     4081 ttgttataaa tctctgttcc atactctgcc tcctcacttt ttacgatatc ctatctctga
     4141 actctggcgc ctgcgccacc catgatagcc tcaacctcgt tgacgcttac catagttgtg
     4201 tcacccggtg ttgtcatggc aagtgcacca tgagctgcac catagtttac tgcagtctcg
     4261 gcatcctctg ttgtcatcag tccatagatg aggcctgatg cgaaggaatc tccaccgccg
     4321 actctgtcca taatctcaag tccgttgtaa tcctttgcct tgtagacctc tccatctgcc
     4381 cagcagattg cgctccagtc attgactgtg gctgtcttta ctgtacgcag tgttgttgct
     4441 actgccttaa agttaggata agcctttgct gcctcattaa tcatcttctt atatccgtca
     4501 agatttaatt ccttgaggtt tgcatcgttt ccttcaatct caaaaccaag gcatgctgtg
     4561 aaatcctctt cgtttccgat cattacgtca acatatttag cgatttcttt gtttacctcc
     4621 tgcgcctttg cctgtccacc gattgcactc cacattgaag gacggtagtt taagtcgtag
     4681 gatacaattg ttccgtattt ctttgctgtc tttattgctg caagcactgt ctcacaggtc
     4741 tgctcagaga ttgctgcata gataccgcct gtatgaagcc atctgactcc aagctcacca
     4801 aagatgtaat cgaaatcaaa atcctcaggt gttgcctttg aaatagcagt atttgctctg
     4861 tcagagcatc cgactgcacc acggataccg aagcctctct ctgtaaagtt gagaccgttt
     4921 ctgcatacac gtccgattcc gtctgtcttc atccacttga tgagggatgt gtccacacct
     4981 ccctgacaga tgaaatcctc catgagcttt cctacctcgt tgtcagcaaa cgctgtgatg
     5041 acacctgtgt tcatcttaaa gcactttctt aaaccgcgga ctacattgta ctctccgccg
     5101 ccttcccatg ctctgaagga tcttgctgtt ctgattcttc cctcgcccgg atccagacga
     5161 agcattacct ctcctaatga aactgcatca tatttacatt cactctttgg ctttaaatta
     5221 aggttcatgg ttttatactc tcctttactt tctgatactg tgtgcaagct ctacggcttc
     5281 ctttgtcatc tgctttatct tgtcaaactc tccggacttt atcatatcgc cctttaccat
     5341 ccagcttccg ccacagcaga taatcttgtc acagcttaag taatccttta agttcttcac
     5401 actcacacca cctgtaggca tgaacttaag catagtatat ggtgctgcaa gagccttgat
     5461 tgtagtcact ccgccatact gctcagcagg gaagaattta accacactaa gacctctctt
     5521 gacagcctgt gctgcctctg aaggtgagat tacaccaggt aaaatcggga tatttttatc
     5581 cagacagtaa tcaacaattt ctgcatcaaa gccggggctt actataaact tcgcgcctgc
     5641 tgcaactgct ttatccacct gctcggttgt aagaactgtt ccagcaccta caagcatatc
     5701 aggatatttc tctgtcataa tgcggattga ttcctctgcc gcagcggttc tgaaggttac
     5761 ctcagcacaa ggaagtcctc cacctacaag agcatctgca agcttttctg catcttttac
     5821 atcgtcaagt accactacag gtacaacaag catttctttt aatttctcaa taattgtatt
     5881 cataataatt cctttctgta tccaaatatc ataaaatcaa caggtattaa tccatgtaag
     5941 cacccttcat agagcttact gcatgctgcg catagagctt taaaagtcca tgctggtatt
     6001 tgctcttaaa gcccttccag tgcgcctttc tgtctgctaa aacagcatca atctcctttg
     6061 gagtctttct ctcgcccttt attcctacaa ttgcgatttt tctgttttca acatcaagct
     6121 cgatcaaatc tccctcttcg acaaatgcga ttggtccgcc tgccgctgcc tcaggtgata
     6181 cgtgtccgat aaccggacct cttgatgcac cggagaatct tccgtcggtg atgagtgcca
     6241 cgcttgaagc aagctttgga tctgcgcaga ttgcctcgcc tgtgtagaac atctcaggca
     6301 ttccgctgcc tcttggtccc tcgtatctga taaatacggc atctcccggc tttacctccc
     6361 catttaatac ggcactgata catgcctcct cgcagtcata cggcttagcg ttaagcactg
     6421 ccttaaacat ctccttagga catgctgtat gctttattac acagccctca ggtgccagat
     6481 ttcctttaag aattgcaata cttccgtctg ttcccttggc attgtcaaag gaatggatga
     6541 tatcctctct gtcaattgct cttccaaaaa tctttgactt ctcacggagt atctcgtcac
     6601 agtgctcgta gaagccgttc ttcttaagct cctctaagtt ctcgccgaga gtctttcctg
     6661 tgactgtcat cacatcaagg tggagcatgc tctttatctc ctccatcact ctaggcacgc
     6721 ctccagcata gtagaagtac tgtgccggcc agtctcccga aggacggatg tttagcagat
     6781 aatgtgcacc tctgtgcagt ctgtcaaatg tatccgcatc tatccggatg ccaaactcat
     6841 gtgcaattgc aggtaaatgc atagttgcat ttgtagaacc tgagatagcc gcatgaacca
     6901 tgattgcatt ctcaaagctt tcctttgtaa caatatcctt tgctgtgatg ccctttgcta
     6961 tgagctccat aagctgcttc cctgcattgt atgaagcctc cttaagctca ggtgctgtgg
     7021 atggcatgag tgctgtaccc ggaagcataa gtccaagtgc ctctgccata atctgcattg
     7081 tagatgctgt tcccatgaat gagcaggctc cacagcttgg gcatgcattg tgcttgtaat
     7141 aatccaactg ctcgttaggg atgacaccgg ttttctccca tgcatcaaat ttaccaatct
     7201 gctcgagtgt cagaagctca ttaatcttgc aggcaggatc atctacgata tatttctttg
     7261 gcaactcatg tgcttccatg actccgcctg tcaccataat cgcactcata tcctttaatc
     7321 ttcctatact catgagcatg gcaggcattg ccttgtcaca gcttgcgata aacactccac
     7381 cgtcaaatac tgttgcattg gcctgtgcct caacaaggtt ggtgatagca tcacggtgtg
     7441 ccagtgaata gttgattccg tcatgtccct gtgcgattcc gtcacacata tctgttgcat
     7501 aatatctggc tgcttttccg ccgttttcat ccacggcctt tacagcctcc tctacaaatt
     7561 tgttcaggtg agctgagccc ggatggctgt ctccatatgt gctttcaatc attatctgtg
     7621 gcttagacaa gtcactgacc ttccatccca tacccattct gagtgggtcc atctcaggtg
     7681 caagttttct tacttcttca cttctaagca tatctgtgtc ctttcttcta tacgaataat
     7741 gaaataataa acgctactac aaatccgctg aagccaacca gtgtctccat gattgtccat
     7801 gtcttcagat ttgttttctc atccacacca acgagtgatc taacaagcca gaagcctgaa
     7861 tcgttgaaat gtgaacatac tgttgagcct cctgctactg ctgcgacaac acatgccaga
     7921 tatagcggtg gcagctctga tattccaggc attgcagcta tgattcctgc tgccatggtc
     7981 attgcaactg ttgctgaacc aacacagatt cgtacgagcg cagctacaat aaaggctaca
     8041 agcactattg gtaaattagc agatgcaact gcatttccaa tcaaatctcc gagtcctgag
     8101 tactgcaata catatctgag cactccacca caggctgtta caagtaagat aagtcctgtc
     8161 ggctcaagag actttgtcat gattttttca agctcctcca tgctgtagcc atgtctcttt
     8221 ccaagcacaa gcattgccac gattgttgca ataagcagtg ccacaaacgg ctcgccaagg
     8281 aatgtaaata ctggtgctac cggtgccatt gcagggataa cacctgcgag tgacttgaaa
     8341 atgataagca caagcggaat tgctatgata agtacgattg tgctgaactt aggaagctta
     8401 gattcatcta tgtcctcctg acttgctaca tgctccggaa ccggaaccat gtattttttt
     8461 ccacaaatcg aaccccatac aggtcctgca ataatcattg caactgttcc acacgctata
     8521 ccgacaagta ttacccatcc taaatctaca ttgagcattg ttgccacaag tacaggaccc
     8581 ggtgtaggcg gtataaatgc atgtccgact gcaagtcctg ctaaaagagg tatcgcatag
     8641 aacagtgtgg aacgctttgt tctctttgca agcgagaatg caagtggtat caggataata
     8701 agtcctgcgt caaagaatac cggcattgct ataattaaac cggttactcc aagtgcccat
     8761 gcggcttttt catcgccaaa ctttttcacc attgtaacgg caagtgtctg tgctccgccg
     8821 gacgcttcca gtattgctcc aaacatggag cccagtccta caagcagtgc tatgcccttt
     8881 aaagtattac tgataccatc atttaccgct gttacaatct cggtaagagg catacctgcc
     8941 acaacaccga ttgttatcgc tccaagcaat atggaaatca ttgcatgaac cttgaatttg
     9001 ataattaaaa ctaataacag agctaatccg attatcgcgg ctatcaccaa tcgggttgga
     9061 tctaatgtcg ctgcttcact cattttttct tcctccataa cattaaattg ttatacgttg
     9121 ttccctacat acatcagacg tcttttgtat ctatctaaag tatataatct taaatacttc
     9181 atatcaagtt gtttcaaatc tacaagtttt ttccacaaaa gtaacaaaaa aaaccagtca
     9241 tgtctgttta acatgactgg tttttagtgt attttattta attctcgtgg taagtttaag
     9301 ctaacttgtc tgacaagttt tattttgctc tgacatgtct ataatagtct gtctgttgta
     9361 tgtgagatgc attatcatgg cacactttgc acccatggtg tctccactta aaatagcatc
     9421 ggttattgcc ctgtgtgtat caattgtctc gttgatcagc tttctgtgtg tcagatttgc
     9481 aaacgtcgta atagctgtta gaataatcgg aatcagtacc tcaacaactc tatttccact
     9541 gcatctggct atcgtctcat gaaactccat atcctttttt atatggttct gccccgacag
     9601 atacagctgc tccacttcat cacacagact ctttatacgc tttttgtcct cttctgttgc
     9661 cttctggcat gccactgcag ctacatcagg ctcaagcagc atcctcacct caaaaagctc
     9721 catcgccaga cgatacttgt cctcaaactg tgctaagcca agcggatcat caaccactat
     9781 ctccgtggac ttcacaaatg tgccggagcc tctcttcacc tcaagcatcc ctttcgtaac
     9841 cagacttttt acagcctccc ttatcgtact cctgcccact ccgaatatct cagccagctt
     9901 gtactcactg ggaagctttg cgccaattcc aatattctca tgctgtatat acattacaag
     9961 ctgatcctca atctgtggaa ccagaagctt ttgatttact ttttcatagt tgtatggcat
    10021 atgctgctcc ttttcatttc atgtgtagat tttaccactg taaatattct atttcaatct
    10081 tctattttaa tctcatacat tcacttttat tttacatttg ctacttcgga tgttatcata
    10141 gtctataaat aaaatgtgta attccaagaa gggagggtgc ttattttgaa gctaaacctc
    10201 aacattcctt tgcagtccct ttcaaccaag gagcttgata tactgcaata tgtacatgat
    10261 cattcagata acatttcatc tatcagcatc cagcagtttg ccaatgagct taactattcc
    10321 acctcaacga tacttcgctt ctgcaggaag cttggcttca gtggcttttc ggagtttaaa
    10381 tatttcctta aaagctctga taatgaggac aataacaaga tcaaaggctc tgataaatca
    10441 cttgatataa tcaagaatac tatcaaagac tcaatggaca gcactgtgag cctgctcaat
    10501 tcagatgaca tgtttcagat tgcaaggctt ttttcagaaa accgtcctat ctacctgcac
    10561 agtcctgccg gacttacaga cattgcagtc agctatctgg aaagcatgct ttttttatca
    10621 ggctgccaga aaatatacaa aaccactgct gccaaggccg cacaccactt tatacagacc
    10681 gtggataagg gaaatgtttt tgtttttata agcaattcag gacgctttga gcccacctta
    10741 aatctggcta aggaagcccg actgcatggc atgattgtaa tctcaatctc atccatagaa
    10801 aataatgacc tggctgaagt atctgattat aatctaaggt ttttttcaaa gccccgtgaa
    10861 tatgaaggcg ccgatttcac ctcacgtctt ggctccttct ttgtaataag ctcattcatg
    10921 gagtatttca acctgcaaag gaaaggagat aacagcaatg aaccttctgc atcagattga
    10981 taccacagac ttctcggaaa acgagctgct catacttgag tattttatag cccatgaaaa
    11041 gagccttcca tacatgtcct taaatgacat atgtaaggat ttatttatct caaatgctac
    11101 aatagtcaga ttctgccaga aaatcggatt caatggcttc aacgaactaa aatactccct
    11161 gagaaaggcc tcaagagaat caaccggatt ttccacctgg agcgttatgc agcatcatac
    11221 ctccatactt aaggatttta ttgacggact tgattcagcg cagcttgaaa ccatatgcaa
    11281 ggaggtaact caaagcgact ccctttatat atatggtcgc ggtctttcct ctctgcccgc
    11341 tagatatttg tactccatgt tcaatgcgat agatattcca tgcatatata ttgactggat
    11401 tgactttctt ttagcaatat cagtctcttt ccccgaaaat gcaaccttga ttttatttac
    11461 aaattatgga gaaaaggaaa tatatgagcc tatcgcaaag cgctgccata caaagaatgt
    11521 gaaaatcata tggatttcaa gcgtcgatat tgattcttcg cttctggcag catctgacat
    11581 ctgcatcact gcgccggagc ttccatccga aaacacgcat ctgcgcacta aaatgacagc
    11641 atttgtattt gtccagatta ttatcgaata tattttaacc caaaaataag ctttttttaa
    11701 taaagagcag gaaaatcatt tgcaattttc ctgctctttt catttagctt ttaatgttat
    11761 atctgctttt aatttttagt atgtatcgat agcaaacagc ggttcacctg ccttgatttc
    11821 gccttcgttt ataagtctga tgctctgatt ttccttgagc tctgtacaaa taacaggaga
    11881 tgctatagat ggtgcatttt ccctgagaaa atccagatca agctgcataa gcacatcacc
    11941 cttatttaca tgtgctcctg tttccacctt tacatcaaag ccctttccat caagcttgac
    12001 agtgtcaata cctacgtgaa ttatcatact gacgcctgaa tcggttgcaa agcctatcgc
    12061 atgcttttta tcgaatacga ataataccgt tccgtcatca ggggcaacaa ccgttccgtc
    12121 ttttggtgtg acaaccgcac catcacccat cagcttattt gcaaatgcct catcaggtgc
    12181 ttttgacaga ttctcggcta agcctgtcat agggcttgaa ataataaccg tactcttcgg
    12241 cttctcatcc tgtacctttt ccacctcagc tgcgcagtcc ttatcattta gtgccgcgcg
    12301 cacagcatca gctattttct caacctttgg tccgtaaacc acctgaatgt gtgtatcact
    12361 tgtctttaca ataccgagtg agcctgtttt cttaagtgtt tcctcattaa taagcgacat
    12421 atcctttaca tcaacacgca gtcttgaaat acagttgttt acaactacga tattcgccat
    12481 tccgccaagt cctgcaataa cctgcaatgc tgtcttatta gagcctgact tcttgccttc
    12541 atttgtcttt tcatctgact ttgcatcaga atcatcatca tctgatacgc tgactacaat
    12601 cttctttctt gacagatacc atttgaatac aaaatagtaa ataaatgcat atggtattcc
    12661 gacaactatt acccagtacc agtgtgagcc cggctggaat actccccata gcaggaagtc
    12721 gataaatcca ccacccgtgt taccaacaat tactccaagc aatgacatga gcatgaatga
    12781 aataccaccc atgatagcat ggaaaacaaa taatgccggt gctataaaca taaatgagaa
    12841 ctcaagtggc tctgtaatac ctgaaacaaa acatgctgcc acgccggcaa tcatgagtgc
    12901 ctttaccttt gccttcttat cctgaggtgt tgtacggtaa atagcaagtg ctgctgctgg
    12961 aagaccaaac agcatataag gcatctttcc ctgtcccaaa aactgtgtat atggcttcat
    13021 atcagaaaga ggtgcctgat ccaaaaactg taagaaaata ttcagacagc cctctactcc
    13081 atggtatgta ccaccgattg ccgtggttct gaacaatgag ttaagcacat gatgaagacc
    13141 tgtaggaatc agcaatctct ccagtgttcc aaaaataaat actccaaacg cacctgatga
    13201 acggataatt cttccaagtc cgtcaattcc aacagaaatc ggagcccaga taagaggcat
    13261 cacaagtcct attactgtca ttgacacaag aacaacaagg gcaacaaatc gcttgcctcc
    13321 ataaaatgct attgctgctg gaaactgtac cttgtgatac ttgttatgca gctttgctgt
    13381 cacaagacca gccattattc cggcaagagc tcccatatca acggtatcca cgccaagaat
    13441 atttgatatt ttcatggcac tgaaatccat tattcctgtt gatatcatac agctgctggc
    13501 aaccagaaat gtgtaatatc cgataaaacc ggcaaatgct gcaactccct tatcctcttt
    13561 agccagacca aatgctatag aaatagcaaa cagaaccggc aaaagggtaa atacaatgcc
    13621 cgatacctta ttaagtgttg ttatgataaa aagcggtacc gatgcggtca aaaatggtac
    13681 ggcatcctgc acctgcgttt taagcagtgc agatgtgatt cccagtatga taccacacgc
    13741 tgcaagtgca gcgaccggca tcataaggct tcttcctaaa tcggagaaga acttcattat
    13801 tttacttttc atatatttta cccatcccta tcctaaagtt tttcacgcaa tacattattt
    13861 taattcaggc cacattcctt tgtttgcctc aataagagca tcaagaatct ttcttgccac
    13921 cttagcgtca ttgataagac ggttaagtgt aaatgcctca agcgctttct gataagaatg
    13981 ctcataatat gcgtctacga gaagcttctc gcatgaaacc tgctgtacaa gaagtccaag
    14041 gtagaactgt gggatatttc caacgctcat aggacgtggt ccgttgatac caagctcaca
    14101 aactacctca accattgcat catcctgcat gtttgcaata gcaccattat tttcaacgat
    14161 aaggatgaaa cgtcctaatg tattgaatgc aatagcctct gcaaccttaa tcatcatctc
    14221 tgcgtgggca tctgagatag catggaattt gtctccgagc tttcctgctt taattacctc
    14281 agcagcctct ttaaatacac gactctcacg gcctgcaatt acctcatccg cacgtgtata
    14341 ttcagggttt aaatggcttg tcttgtactc agggtacagg taatatccgt catatgtatt
    14401 aggaaggtaa tccgggaaat cctccatcat tgtctgtaca aagccatatg tatcaagcca
    14461 tgactgatca cgctgctctg catcctgtgg aaggaagcct ttttccttta caatctgacg
    14521 aatctttgga agtaaatcct ctcctgtctt gcggtcatac atatgtgtaa accatccata
    14581 atggttcaag ccgaagtata caggatcaag atcgccccag tcgcagccta acagacgact
    14641 tgtcgaacga actacattct caggctggtc acagatattg aggattctct tatcatcagg
    14701 gaactctctt ctgagtgcct ctgcaacgat tgcagccggg ttagagtagt taagtatcca
    14761 tgcctcaggt gagtactcac gaatctggtg aacagcctca atcatgtcta cacatgaacg
    14821 aagtccgtat gccattccgc ctgctccaca tgtctcctgt cctaccttgc caaagctgag
    14881 tggaatatgc tcatccttct gacgcatatg aagtccaccg gcacgcatct gcataaagat
    14941 gaaatccata tcctggtaag cttcctttgg atctgttgtg taagaaaaat caagctcagg
    15001 atatctctct gcaaacatga tgtctccata ctgtccgatt ggcttttgtc tctcgccgtc
    15061 aatatcaaat aatacgagct tatttaatgg aaactcctcc tgcatacgtg caaatgattt
    15121 taaaaatcca agtgtatatg tgcttcctcc acctacaata catacgttat actttttcat
    15181 gatagtatct ccttttctca ttctatttat tgcaatatat ccggccagat ttatttgcgt
    15241 ttcttattta cacacatact ataaccaatg cacacagcaa tgtaaatggt tttacatttt
    15301 gtagttttgt atacgcattt ttgtgattta ttacttttta tgtaattttt tacatagatt
    15361 taaattgaat catttacaca aaaaaaacac tgccggttat atcccgacag tgcaataatt
    15421 atctacacaa cacaaattct ttgaaccttg ggagtgtaaa ctccaaatgt ccatactccg
    15481 gtgaaataat caagcctttt tttataagcc tgttcctgta tacactgaaa ctgtttgatg
    15541 ccttttttat agtctttcgt attgcttcca ctttggtgtc attggttttt gccatagcaa
    15601 ccaacaattc cttatcacct tttgacagct cactccatat cttttcatat acatattcct
    15661 ccagatactg actgaattca ggtaatacat tatgccatcc ggtattatgc ttaaaacaaa
    15721 gataccccag tacctggaag gcaaacggat atcccctcgt ctctttagcc atctctgtag
    15781 cttcctcatc cgtcaattca aaaatagact tgtatttttc agctatcatg cctatattca
    15841 acggacgaag ctcaatttta ggtgccctgt acaaaaatgt gagcgttttt tcattctgaa
    15901 gctcataaat attctcataa agacctgtca taagcaaaaa taccggaaga tcctgtctca
    15961 tatatatctg aaacaggctg acaaactctt tcattgtatc attacataca gcctcgtcta
    16021 ttgttacaag aactctctta cgcttctttt taatcttttc aagcatcctc gtcactgcca
    16081 catttaaatc ggtaatcgga gaaacacctt ctatttcaac gcccagccct aagaatgaca
    16141 gatttatttt agcttcacga aaaacctctg acaaacttgg ataattgctc agatttgcag
    16201 caaatgattg taataaatca cgttcagggc tcagatcgat aacaatccag tcttcctctg
    16261 ccctaaaagt tttattgatt tctgtgagca ttacagtttt ccctgagcct ctgactcctg
    16321 taatcataca aacctggtaa gtagggtttt catcctcaaa tgtatcaatt atttcataag
    16381 actgaacatt acggttaatt aaactgaccg gttccttgcc aaatgataat gaaaagggat
    16441 tatttttcat ctctgcgctc ctgttttata actctttata acttttaata atttttataa
    16501 ctctttataa ctttgtattc tttttataac tctttataac ttatttgtca aggtcaggtt
    16561 ccatacgtac cctacagtct aatatgttcc ctacagcctc ctgtagttct caagaagctt
    16621 gtgcagatca tcaatctctc caataagcct cttccctata tcggtatcct tcacaagcat
    16681 acgtctctta tcattttcct cgacctctga tgatgtctct atgtcagtat tatttatcag
    16741 ggcatcctcc accgatgtaa aaggcgtgtg tgccttgagc cttagaccat gtgaattgta
    16801 aatcagtgta tagccggcga taccggtttt cttgttcatc gcacggcaga agcctccatc
    16861 tatcacaaac agcttgccat tggccctgac agggttctct ccctctattg cgtgaaccgg
    16921 tgtatgtcca tttatgatat gcgcatcatc atcatacagc ccgaactcgt gcagtatctg
    16981 acagcagaag ctcttatcgt aatacctgct gtaatatggg ttagaaggct cttcccacat
    17041 gctcttgtct gtaacatatt ccatctcaaa ggtcttaagc cttctgccgc acagcggtga
    17101 tttttctcct ccccacaaaa accacataaa gtccacatca tccttataag gattgtccac
    17161 atacgcagcc cttgccttct catcacattt gtcaagcaac gcccttccgt gataaaattc
    17221 accatcaata tacagcttat caaaggctcc gttgctatca accggaacac agccatgata
    17281 gagcaggttg ccattgtata cattgtacat gccgccctta tcatacaaaa atctgacatg
    17341 cttgtgcagg cgctgtcctg tcacaaactg tctgtgcagc tttctcatga tgacagtctc
    17401 ctcgtcactt agtgcgtagg gtctctcatc atcaacggtt ggaaagcgct ttctgtccac
    17461 tcctacatcc tgcagctcgt ccagcctgtc aagcatcagc ctgtttccca tctcatactc
    17521 gggatgtgca agtataagct gcccctcaag cttaaaaagc attactgtga tggccgccct
    17581 tgcagcctca attccatcct ctataccata tgttttcatg ccaaacagca ttagctgtcg
    17641 taagcttatt ccgtaggcat tctcaagaat ttccacattg ttgtatttaa gattgttgcg
    17701 gatgacatta catatacatg catcatttcc acatgccgca cccatccaga gaatgtcgtg
    17761 attgccccac tcaatatcaa gcgagtgata atcataaagc agatcgagaa tcttatccgc
    17821 gcttcctcct ctgtcaaata catctcccac aatgtgcaga tggtcaacag caagcctctt
    17881 aatcagtgca gacaaagcga ttataaactc atccgcatcc tccaaatcaa ttatagtctt
    17941 taatatctgc ttgtgatatc tgacctggtt tgcatcctca tcctcctgcg catgcaaaag
    18001 ctcatcgata atgtacgcat attcctcagg cagagccttt ctgaccttcg acctggtgta
    18061 ctttgatgag agcagctttg cgacaagtat aagctgattt aacgtcatag cataccagtc
    18121 actgtctatc ttgccctgtt catcaagcag ggccattttc tctcttggat agtatatgag
    18181 cgtgcaaagc tcctgtctgt cataatcaga aatcgtatcc ttgaaaatca aatccacctt
    18241 ttcctttatc acacctgagc agttgttcat gatatgtaaa aaagcctcat actcgccatg
    18301 tatatcactc aggaaaaact ccgtgccctt tggcaggttt ataatcgcag aaagattgac
    18361 tatctccctg attacatcct gacttgatga ataattttgt tttaaaagct tcagatattt
    18421 tgtattacct ttatcgttgt acatatttcc cccgtaacct cttatttttc aatgaaatat
    18481 attaattcgc tcctattcat gttccatgct ataccggata aatcccttaa attacatttt
    18541 tttcattata taataatatt tttcaccccg caaccaatcc tgtcatttgt actaaaaaag
    18601 atgcgcaaaa ctaatactat tttacgcatc tttgacatat tttatattct gaataattac
    18661 ctttatttca tgtactcagg tgtcacattc ctgcccatgc cctcaagcat ttccttgtca
    18721 cttctgattg tggcacacgc tactgtcgta agcatgtttc ctgtaattgt ggctgatgct
    18781 cccgacttga atgcaagctc tccgtcattt gtaagcagcg ctcttccggc tccgagacgt
    18841 atgtcagcct ttggatttat atatctgaaa aatgacactg tacgcagtat atcaggctca
    18901 ctgatatgct caagtccctc aagcggagtg cctgcaatcg gcataagcgc attaagcgga
    18961 atggaatcca ctcctacctc tgcaaggct
//
LOCUS       DPUF01000165            5452 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_15264, whole
            genome shotgun sequence.
ACCESSION   DPUF01000165 DPUF01000000
VERSION     DPUF01000165.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_15264"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="DHV81_05860"
     CDS             complement(<1..278)
                     /locus_tag="DHV81_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCI93999.1"
                     /translation="MDPITLAQEIIDGRRITRDDDLSFFLTCDLDELCKGADMIREKF
                     IGDKVDLCSIINGRSGKCPEDCKYCAQSAHNHTNCEVYDFLPEEKILEA"
     gene            complement(428..>709)
                     /locus_tag="DHV81_05865"
     CDS             complement(428..>709)
                     /locus_tag="DHV81_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HCI94000.1"
                     /translation="WIVVFATGKQHTIKHIEKKELGFICLSGLATGGSWLCYYKALQD
                     GLASVVVPIDKLSIVVSIVFSYFVFKEKLTIKSFTGLVLIIAGTLIMLV"
     assembly_gap    710..861
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<862..1316)
                     /locus_tag="DHV81_05870"
     CDS             complement(<862..1316)
                     /locus_tag="DHV81_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HCI94001.1"
                     /translation="MWVLYAFGSAFFAGITSILAKCGIKKTDSNVATAIRTIVVLIFS
                     WIMVFVVKAQGQITAVSAKTWIFLMLSGVATGASWLCYFKALQLGDVNRVVPIDKSST
                     ILTIILAFIFFKEEINVLRLVCVVLIAIGTYMMITKKEISQEEQNKARGS"
     gene            complement(1576..1648)
                     /locus_tag="DHV81_05875"
     tRNA            complement(1576..1648)
                     /locus_tag="DHV81_05875"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(1613..1615),aa:Lys,seq:ttt)
     gene            complement(1666..1737)
                     /locus_tag="DHV81_05880"
     tRNA            complement(1666..1737)
                     /locus_tag="DHV81_05880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(1703..1705),aa:Gln,seq:ttg)
     gene            complement(1806..1879)
                     /locus_tag="DHV81_05885"
     tRNA            complement(1806..1879)
                     /locus_tag="DHV81_05885"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(1843..1845),aa:His,seq:gtg)
     gene            complement(1927..2000)
                     /locus_tag="DHV81_05890"
     tRNA            complement(1927..2000)
                     /locus_tag="DHV81_05890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(1964..1966),aa:Arg,seq:tct)
     gene            complement(2032..2102)
                     /locus_tag="DHV81_05895"
     tRNA            complement(2032..2102)
                     /locus_tag="DHV81_05895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(2068..2070),aa:Gly,seq:tcc)
     gene            complement(2106..2183)
                     /locus_tag="DHV81_05900"
     tRNA            complement(2106..2183)
                     /locus_tag="DHV81_05900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(2146..2148),aa:Pro,seq:tgg)
     gene            complement(2359..2952)
                     /locus_tag="DHV81_05905"
     CDS             complement(2359..2952)
                     /locus_tag="DHV81_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCI94002.1"
                     /translation="MTKIIFATGNKDKLREIKEILSDCDVDIRSMKEAGINVDIVEDG
                     KTFEDNALIKARAIAAHTDAIVLADDSGLEIDYLNKEPGVYSARYMGEDTSYDIKNNN
                     LIERLDGVPKEKRTARFVCAIAAVLPDGKELVTRQTMEGYIGWEIAGANGFGYDPIFY
                     LDEYGCSSAELTPQQKNAISHRGKALRAMREMLVKVI"
     gene            complement(3001..4080)
                     /locus_tag="DHV81_05910"
     CDS             complement(3001..4080)
                     /locus_tag="DHV81_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCI94003.1"
                     /translation="MYRNILKIITVFFVMLALIVPVTLEKLPAINEKYDMAKALEDNV
                     LQNYVKASTDAKQEKNPDEILQEKAGFEDDLRIELPDDSEGERQSISVETDALTQTVY
                     VKLKADVDNYFTKYSMTGRSNHIDSMQYYRKGAEGVIAITTDKLYQVKQRIENGYLYL
                     SFVNLHDIYDKVIIIDAGHGGRMTGAVRNGIEEKSINLDIVLALKEQLDSYSGDKRLG
                     IFYTRTTDTNPTLQQRAALANKADADLFISVHCNSYEKGNFTAVSGTQVLYSQSDNRE
                     LGSKHLAQICMDNVTAVTGNRAFGLLPADDIYIIRTSEAPVALVEVGFMTNRQELDNL
                     ANADYQKQAAQGIYNAIMQAFDEGY"
     gene            complement(4082..>5452)
                     /locus_tag="DHV81_05915"
     CDS             complement(4082..>5452)
                     /locus_tag="DHV81_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HCI94004.1"
                     /translation="QNELLVEKHSLTADFKLADDNGFVQYKGQFYASLDKLSKLLDMT
                     CSFDTATNTLTMTDKSEGVSTVPTKYDLRERQRVSLIRDQGSYGTCWAFAATSALESA
                     LMPEEQLLFSVDHMSMSNSFNVNQYDGGEYTMGMAYLAAWQGPVYDADDPYGDGVTRN
                     DLAAVKHVQQMLIIDGKDYQGIKEAVFKYGGVQTSLYSTIASSKTKTPYYNKQTNSYC
                     YMGQDKPNHDVVIIGWDDNYPKENFNVDLEGDGAFICQNSWGSSFGDNGVFYVSYYDT
                     NVGTHNVVYTDIESADNYDNIYQSDLCGWVGKMGYDKEDMYGANIFTAQSAESLRASG
                     FYATAADTSYKLYVVKNFENEYSFKNKTLVAEGSLKNAGYYTVDFDEPVDLESGERYA
                     IVLYINTPGSTHPMAIEYNSGEAYMSTVNLDDGEGYISYDGVAYINVKEKQDCNLCIK
                     AFSDNR"
BASE COUNT         1367 a         1361 c          984 g         1588 t
ORIGIN      
        1 gcctcaagta tcttttcctc aggtaaaaaa tcataaacct cacaatttgt atgattatgt
       61 gctgactgcg cacagtattt gcagtcctca gggcattttc cgcttcttcc gtttattatc
      121 gagcacaaat ctaccttgtc accgataaac ttctccctga tcatatctgc gcccttacac
      181 agctcgtcta aatcacatgt aaggaaaaat gataggtcat catcccttgt gattcttctt
      241 ccgtctatta tctcctgtgc cagtgttatt ggatccattt taatctcctt cgtgtcctgt
      301 atttcttctc tcatttaata agtatcatta taatacatat catccgttat aattgtcaac
      361 tataaatatt tcctcggtta acaatgataa tagaaaaaca gaaaaacgtc aagatgtttt
      421 tatatattta aaccagcata atcaatgttc ctgctatgat gagcaccagt cctgtaaagg
      481 atttaatcgt cagcttttcc ttaaatacga aatatgaaaa cacaattgac acaactatgc
      541 tcagcttatc tataggcact acgacactcg caagtccgtc ctgcagtgcc ttgtaatagc
      601 aaagccatga gccgcctgtt gcaagtcccg aaaggcagat aaagccaagc tcttttttct
      661 ctatatgctt tatggtgtgc tgttttcctg tggcaaatac cactatccan nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn ngaacccctg gccttattct gctcttcctg tgatatctcc
      901 tttttggtga tcatcatata cgttccaatt gctataagca ccacacatac cagcctgagt
      961 acgtttatct cctctttaaa aaagataaat gcaagtataa tggtcagtat tgtgcttgat
     1021 ttatcaatcg gcaccactct gtttacatcg ccaagctgga gtgctttaaa ataacagagc
     1081 catgaggctc cggtagcgac tcctgaaagc atcagaaata tccatgtctt tgctgacaca
     1141 gctgtaatct gcccctgcgc tttaaccaca aataccataa tccatgaaaa aatcaaaact
     1201 acaattgtcc tgattgcagt tgctacgttt gaatcagttt ttttgatacc acactttgcc
     1261 agtattgatg ttatacccgc aaaaaatgcg gatccaaatg catataatac ccacatattt
     1321 ttctctgtct acgcttcggt caactacgtt gtccggagtt cgaaacctcc attcttctaa
     1381 atacaaaaaa accgttcaag gaacggcttc ttttacgcta atgagacatc ggggattcgg
     1441 tctacgctcc gcttcggtcc gcgcttagca gacgtccccc ggacgtctag cgccccggtc
     1501 aactccgttg tccggagttc gattcctacg atgtctaaca ttaaaaagtg cctctccaag
     1561 agagacactt ttcaatgaga catcggggat tcgaaccccg gacaacttga ttaaaagtca
     1621 agtgctctac cacctgagct aatatcccat atattcagat tgtaacaggg ctagttggat
     1681 tcgaaccaac gaatgcagga atcaaaatcc tgtgccttac cgcttggcga tagcccttga
     1741 gtaatactcg tgcgccgcac ggatacgcat ataatgcgca cgacactatt tgcactaatg
     1801 caaagggtgg atacagggat tcgaaccctg ggcctccaga gccacaatct ggcgcgctaa
     1861 ccaactgcgc tatacccacc atatttactt catatggtcc atatggaatt gatatgaaat
     1921 aaaaaatgtg cctgaaggga ttcgaacccc cgacacccgg cttagaaggc cggtgctcta
     1981 tccaactgag ctacagacac atatgggata ctgcgaatgc tgcaatatct aagcgggtga
     2041 tgggaatcga acccacgtat ccagcttgga aggctggtgt tctaccattg aactacaccc
     2101 gcatgtagtc ggggtgacag gattcgaacc tgcgacctcc tggtcccaaa ccaggcgctc
     2161 tagccaagct gagccacacc ccgaaggatt tgtttgcgtt ctttccgtga cgcaagaatt
     2221 agtatatagc aaagggcaga atatgtcaac aacttttttt atttttttta tatttttttt
     2281 aaagtggctg ccggacagct taaaagagct tcccggcagg cattttatcc gtatacgttg
     2341 caattcaacg cctatactct agataacctt cacaagcatc tctctcatgg cgcgaagagc
     2401 cttgcctcga tggcttatgg cattcttctg ctggggagta agctctgcag aagagcagcc
     2461 gtactcatct aaatagaaga taggatcata accaaagccg ttggctcccg ctatctccca
     2521 tcctatgtag ccctccatag tctgtctggt cacaagttcc tttccatccg gcagcactgc
     2581 ggcgatagca cacacaaagc gggctgtacg cttctccttt ggcacaccgt caaggcgttc
     2641 tataaggtta ttattcttta tatcatagga agtatcctcg cccatatagc gcgctgaata
     2701 tactcccggt tccttattca gatagtcaat ctcaagtcct gagtcgtctg cgagaacaat
     2761 tgcatctgtg tgtgccgcaa ttgcgcgtgc cttaatcagc gcattgtctt caaaggtctt
     2821 cccatcctcc actatatcca cattgatacc tgcttccttc attgaacgta tatccacgtc
     2881 acagtctgaa agtatctctt tgatttctct gagcttatcc ttgtttcctg ttgcaaatat
     2941 gatttttgtc ataatatatt tccttccttc tttttctatt cggacaattt ccagatacag
     3001 ctagtagccc tcatcaaagg cctgcatgat tgcattgtat ataccctgcg ctgcctgttt
     3061 ctgatagtct gcattggcca gattgtcaag ctcctgtctg ttggtcatga agcccacctc
     3121 cacaagcgca accggagcct cacttgttct tattatatat atgtcatctg caggaagcag
     3181 cccaaatgcc ctgtttcctg tcacagctgt cacgttatcc atgcaaatct gtgcaaggtg
     3241 tttacttccg agctctctgt tatctgactg gctgtagagc acctgcgtac ccgaaaccgc
     3301 ggtgaaattg cccttttcat atgagttgca atgcacgctt ataaataaat ccgcatccgc
     3361 cttattggca agtgccgctc tctgctgcag tgtaggattg gtatctgttg tcctggtgta
     3421 gaatattcca agccttttgt cacctgaata gctgtcaagc tgttccttaa gtgccagcac
     3481 tatgtccaga ttgatgcttt tttcctctat tccgtttcgc acggcaccgg tcatacgacc
     3541 gccgtgacct gcatctatta tgatgacctt atcgtatata tcatgcagat tgacaaagct
     3601 cagataaaga tatccattct ctatgcgctg cttcacctga tagagcttgt ctgtagtgat
     3661 agctatcaca ccctctgccc ccttgcggta atactgcatg gagtctatat gatttgacct
     3721 gcctgtcatg gaatacttcg taaagtaatt gtccacatca gccttaagct ttacatagac
     3781 cgtctgcgtc agggcatcgg tttccaccga tatactctgg cgctcccctt ctgagtcgtc
     3841 cggcagctct atccttaaat catcctcaaa gccggctttc tcctgcaata tctcatcagg
     3901 gttcttctcc tgctttgcat ctgtggaagc cttgacatag ttttgcagca cattatcctc
     3961 cagtgctttt gccatatcat acttctcatt tatagccgga agcttttcaa gcgttaccgg
     4021 cacgataagt gcaagcataa cgaaaaatac tgttattatc tttagaatat ttctgtacat
     4081 atcatctgtt atcagaaaag gcctttatac agaggttaca atcctgtttt tcctttacat
     4141 ttatatatgc cacaccatca tagctgatgt agccctcgcc atcatccaga ttgaccgtag
     4201 acatgtatgc ttctcccgag ttgtactcta tagccatagg atgagtcgag cccggtgtat
     4261 ttatataaag tacaatggca taacgctcac cggactccaa atcaacaggc tcatcaaaat
     4321 caacagtgta atagcctgca ttttttagtg acccctcagc caccagcgtc ttatttttaa
     4381 aagaatactc attctcaaaa ttcttaacta catacagctt gtatgaggta tccgctgctg
     4441 tagcataaaa gccggaagcc cttagtgatt cagccgactg tgcggtaaat atgttggctc
     4501 cgtacatatc ctccttgtcg tagcccatct ttcctaccca gccacacagg tcactctggt
     4561 atatattatc ataattgtca gcactctcta tatctgtata aacaacattg tgcgtaccca
     4621 catttgtatc gtagtatgac acatagaaca ccccattgtc tccaaaactg ctgccccagc
     4681 tgttctggca tataaaggct ccatctcctt ccagatccac attgaagttt tccttagggt
     4741 aattgtcatc ccagccaatt ataaccacat catgatttgg cttatcttga cccatataac
     4801 agtaagaatt agtctgctta ttgtaataag gcgtctttgt ctttgagctt gctattgtac
     4861 tgtaaagcga tgtttgcaca ccaccatatt tgaaaacagc ctcctttatg ccctgatagt
     4921 cctttccatc tatgataagc atctgctgca catgctttac tgcagcaaga tcattgcgtg
     4981 tcactccatc tccgtaagga tcatccgcgt cataaaccgg tccctgccat gctgccagat
     5041 atgccattcc catggtatac tctcccccgt catactgatt gacgttgaag gaattgctca
     5101 tactcatatg atccacggaa aacaaaagct gctcctccgg cataagggct gactcgagtg
     5161 cacttgtggc cgcaaaagcc caacatgtgc cgtagctgcc ctgatccctg attagtgaca
     5221 cacgctgtct ttccctcaga tcatactttg ttggcaccgt tgatacaccc tcggatttgt
     5281 ctgtcatagt gagcgtattt gtcgccgtat caaagctgca ggtcatatcc aaaagtttag
     5341 acagcttgtc taagcttgca taaaactgcc ccttatactg tacaaagcca ttgtcatctg
     5401 ccagcttgaa atcagctgtc agactgtgtt tttccaccag aagctcattt tg
//
LOCUS       DPUF01000166            8676 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3124, whole
            genome shotgun sequence.
ACCESSION   DPUF01000166 DPUF01000000
VERSION     DPUF01000166.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8676)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3124"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="DHV81_05920"
     CDS             <1..627
                     /locus_tag="DHV81_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HCI94005.1"
                     /translation="DFSEDTSSFKVPCVIVTTSAENWSMEELSSVSIDDEKAGETIVD
                     YLIEHGHTKIAEIGANLELSQTARLRHNGYLNSLKKHGITPDERYYERARFSYDSAYR
                     AMKKLLEKDIEITAVYAISDVMAIGALRAILDKGLRVPEDISITGFDGSTLAEYYNPK
                     IVSIRQPHKDMAARSVELLFNMIDLHKGSCHEIIPFELTEGESVARID"
     gene            688..1854
                     /locus_tag="DHV81_05925"
     CDS             688..1854
                     /locus_tag="DHV81_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCI94006.1"
                     /translation="MEVNNKMKHTIKVAWAKGFSQFLALAGAILLVFVLFKFDAIMAQ
                     IVRIINILMPIIMGIVLAYLINPMVEFYDRKLSNSLGKAIEKKCGKRPSMRGLSILIS
                     LAIIIAIIVVLIMMVVPQLVSNITNAVSLLPDQIQDLVKKITELAKNNDRAKEIITEL
                     YNNGISYFTEWVRDNMLGQVTFVINGIMGIFGTAVNCLVAFIVAIYVLLSKDTFKRQT
                     KKLVSAFLPERHIQVLSSILKESDKIFGGFISGKIIDSLIIGAICFVCCLILRMPYVA
                     LISVIVGVTNVIPFFGPYIGAIPSTILIMLDSPSKGVIFLLFIIILQQVDGNIIGPKI
                     LGESTGLSPFWVVFAIFLGNGLFGVMGLFIGVPTWGVVYYLIKRYVNYRVRKKE"
     gene            2044..3387
                     /locus_tag="DHV81_05930"
     CDS             2044..3387
                     /locus_tag="DHV81_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI94007.1"
                     /translation="MKTDMTKGNPMKIILLFSIPVLMGNLFQQFYNMVDTIIVGQYLG
                     SKALAAVGSTGCIMFLVLGFANGIAQGFGVMASHAFGAKKEKLLKHYVALAIILTVIV
                     SVVLTIPTVLASRQLLIWLNTPDDILVMADAYIKVIFAGIFCTMSYNVASGLLRSIGD
                     SKTPLYFLIFSSVLNIVLDIFFIVVVKAGTAGAAYATVISQGIAAVLSFIVMFKKYDL
                     LRTSREDYYMDWNGIWRMLSIGLQMALNYSITAIGTMIFQAAVNVFGSQVVAAYTAAS
                     KVSNIATQTMPTLGTAAATYCGQNLGAGRYDRIFKGMKSCFFICIGCAVLAAAINCFV
                     GPYMIGWFITSPTATDIDCAMKYLKIASVFMLPLAWIFAYRNGLQGLNKGLVPMLSGA
                     MELVSRWIAILALSKPFGYGGVCFADPAAWLTTGILLIVTYYVWEMRMRKKIQIK"
     gene            3471..5090
                     /locus_tag="DHV81_05935"
     CDS             3471..5090
                     /locus_tag="DHV81_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94008.1"
                     /translation="MNYYELSNTVTPDTIGYKNGLWQKRYVQIYRVLTVVWSVLTLCL
                     LFGMFHRDDYSSGMIKSCLLLFFAGIIFLVLMLIAVVNISAKRTENWSLQDRHDYNLA
                     MYRTRYRNNRQLQSVVLIVMAKQQLLMSNYDLAAQALAMVDINCVKLPYLRDYYFCNA
                     AVLFLCDKPGWQEWLDKCYAVPANQKQMTDMQIGALFLSENAKMDLCQAIYADTRIKH
                     KWPTAIVITAILVLYAGIFYGVGGLLSRGYHYRYWFELSSVLITYAGCMVLSLYWIIR
                     WILFSDSVSKKSEGVRLFRKVILIVMWLCLMAAMVINLIISFIGMDTEKKVYANGLIC
                     MSTSDGYSTDEYYNIKVGPFLRRNLTFKETIKFGLINDDDSTGDSGNDAGDSGANDSE
                     NSTENNSQSYNDASENTENENAGNENADESLDTESMAVYDYMSAAGILSAGDAGIIRN
                     GSTAKGTFYSVFETGEADGNTYENRLVYDRVSENGQCDLFVYEQLVSGSDTRLLGFYA
                     VNKTTNEVFSADRNSWGGVSSREYQDATGEN"
     gene            complement(5118..>5942)
                     /locus_tag="DHV81_05940"
     CDS             complement(5118..>5942)
                     /locus_tag="DHV81_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HCI94009.1"
                     /translation="IIVPNLYLHYYSEMLTQLLATYTDYHYKFLIFMSDKGAAEEENY
                     IDELLAYQIEGLIVLSHTLSSEKLASYHIPIVAIERESMHISSVTSDNYMGALQATSL
                     LIRDKCDILIHVNVDVPKYVPAYDRIRAFEDTCTEHNIPYELSLNVSGDSYNDLFSQM
                     KVIFNNIDEKYPDKKKGVFLANDTYANMFLNLVIQKYGCLPDSYEIVGFDNSPIANEA
                     IIPITTIGQQIDVIARTAMDLLVKQMDNRKQRKPEPDLAPTHKKIAPVLIRRETTS"
     assembly_gap    5943..6086
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6384..7298
                     /locus_tag="DHV81_05945"
     CDS             6384..7298
                     /locus_tag="DHV81_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI94010.1"
                     /translation="MKEKKQSLKSKVTYVKKNWMLYIFFLMPALLLTIIFKYVPMGGL
                     LIAFKDYNVIKGVLGSPWVGLEYFKRFLSSPDFMNYLMNTLKVSIYGLLWGFPVPIIL
                     ALLLNRIRKEGIKKKIQLLIYAPNFISVIVLCGMVRMFLSPIGPMNKILGISTNWMTM
                     PAAFRTIYIASGIWQGAGWASIMYTAALSNASKELEEAARMDGANIFQQMWYVELPAI
                     KNIIVIQFILQAGNIMSIGFEKAYALQTDMNLPASEILSTYVYRIGLLNGDYGYSTAV
                     GLFNSVINVILLIIVNAVVKKLNDGEGI"
     gene            7311..8213
                     /locus_tag="DHV81_05950"
     CDS             7311..8213
                     /locus_tag="DHV81_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI94011.1"
                     /translation="MEKDMTIKIKYSRTDKIVLGIGYTLLGLFVLAIIVPLVYVVLAS
                     FMDPTVLNNQGLSFNIKDWTIDAYRRVLENDMIWRGFFNSFFYSIAFAAISVFVTILA
                     AYPMSKKEFVGRKFFNVIFIITMFFGGGMIPTFILINQLHMVNTVWAILIPGAFNVWN
                     MILARTYYQSVPNELREASAIDGANEIQHFFKIMLPVCKPIIAVLMLWSFVGMWNSYF
                     DAMIYLNDANLQPLQLVLRSILVQNTPQPGMIADIQSTAEMAKVAEQLKYATIVVSSL
                     PLLIMYPFFQKYFDKGIMVGSVKG"
     gene            8234..>8676
                     /locus_tag="DHV81_05955"
     CDS             8234..>8676
                     /locus_tag="DHV81_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI94012.1"
                     /translation="MKKRSFKKLITLSLTLAMLTGVAGCGKQQSLNAETNPDTPVSDV
                     QFPLKEKAELSFITSAPATSTQDPNERTIFKRMEKQTNVHIDWTCFVSDQFSDKKNLA
                     LAQFGNLPDGLFNAGMSDYDLLRYAKQGIIIPVENLIDKYMPNLQA"
BASE COUNT         2480 a         1523 c         2047 g         2482 t
ORIGIN      
        1 gatttctcgg aggatacatc ttcgtttaag gtgccgtgtg ttatcgtgac cacatcggca
       61 gaaaactgga gcatggagga gctgtcatct gtttccattg atgatgagaa ggcaggggaa
      121 acaatcgtcg actatctcat cgagcatgga catacaaaga tagcagagat tggagccaat
      181 ctggagcttt cacagacagc tcgtctgcga cataacggat atctcaacag cttaaaaaag
      241 catggaatca cacctgatga gcgttactat gagagagcgc gattctcata tgacagcgca
      301 tatcgtgcga tgaaaaagct tttggaaaag gatatagaaa tcaccgcagt ctatgcgatt
      361 tccgatgtca tggcaatcgg tgccttaagg gcaatacttg ataagggact cagggtgccg
      421 gaagatatat ctatcacagg ctttgatggt tcgacacttg cagagtacta caatcccaag
      481 attgtatcta tcagacagcc gcacaaggat atggcggcac gcagcgtgga gcttcttttt
      541 aacatgatag atttgcataa aggctcatgt catgagataa taccatttga gctgacagag
      601 ggcgagagtg ttgctagaat tgattaaaga ctatataata tcagcttgtt acattccgga
      661 catacaataa acgaggagga atcgcgcatg gaagtgaata ataagatgaa gcacaccata
      721 aaggtcgcat gggccaaagg gttttcacag tttttagcac tagcaggagc aatcctgcta
      781 gtgtttgtgc tatttaaatt tgatgcaata atggcacaga tagtgagaat aataaacata
      841 cttatgccaa tcatcatggg gattgtgctg gcgtatctca taaatccgat ggtagagttt
      901 tatgacagaa agctaagcaa ttcgcttggc aaagccatag aaaagaagtg tggaaagaga
      961 ccttctatga gagggttgtc aatacttata agtctggcca taataatagc cattattgta
     1021 gtgctcatca tgatggtggt gccacagctt gtaagcaata tcacaaatgc ggtcagtctt
     1081 ttaccggatc agatacagga ccttgtgaaa aaaatcacgg agcttgccaa aaacaatgac
     1141 agggcaaagg aaataatcac tgagctatac aataacggca taagctattt tactgaatgg
     1201 gtaagggaca atatgctcgg acaggttacc tttgtgataa acggtattat gggaatcttt
     1261 ggcacagcgg taaactgcct tgttgcattc atcgttgcca tctatgtcct gcttagcaag
     1321 gataccttca aacgccagac taaaaagctc gtgagcgcat ttctgccgga acgccatata
     1381 caggtgcttt caagcatctt aaaggagagc gacaagatat tcggtggctt tatcagtggc
     1441 aaaatcattg attcactcat aatcggagct atctgtttcg tgtgctgcct gatactgcgc
     1501 atgccgtatg tagcactcat cagcgtgata gtcggagtga ccaatgtcat accgttcttt
     1561 ggaccgtaca ttggagccat accaagcacc atactcatca tgctcgacag cccgtcaaag
     1621 ggtgtgatat ttttgctgtt tatcattatc ttacagcagg tggacggcaa tattatcgga
     1681 ccaaagatac tcggtgagtc cacaggactc tcacctttct gggtggtatt tgcaatcttc
     1741 cttggaaatg gattatttgg agtaatgggg ctgttcatcg gagtaccgac atggggtgtc
     1801 gtgtactacc tgataaagag atatgtgaat tatcgtgtga ggaagaaaga ataaacgtac
     1861 tgttaaaaaa taagttataa attttgaaaa tagtacttga atactggaaa atatgtgtat
     1921 ataatgtgtg aacgaggctt tgaaaaagag cttgttcaca cattttttta atagattttt
     1981 tgcaggggtg aacctgctgg gaaaagttat tatgaattga atttttgggg agggtattta
     2041 cacatgaaga cagacatgac taaaggcaat ccaatgaaaa ttatactgct gttctcgatt
     2101 cctgttctga tgggaaatct gtttcagcag ttctataata tggtggacac gattatcgtg
     2161 gggcagtatc tgggctcaaa ggcgcttgcg gcggttggct cgacaggctg tattatgttt
     2221 ctggtgcttg gctttgcaaa tggaatagca cagggctttg gagttatggc aagccatgcg
     2281 tttggtgcga aaaaagagaa gcttttgaag cattatgtgg ctttagctat cattcttact
     2341 gtgattgtct cagtggtgct tacgattcct acagtgcttg cgtcgaggca gcttcttatc
     2401 tggctcaata cacctgatga tatacttgtc atggctgatg cgtacataaa ggtcattttt
     2461 gcgggaattt tctgcaccat gtcctacaat gtggcttccg gtcttttgcg aagtatcgga
     2521 gacagcaaga ctccgctgta ctttttgatt ttttcatctg tattgaatat tgtgctggac
     2581 atattcttta tcgtggtggt gaaggcaggc acggcaggag ctgcttatgc gacagttata
     2641 tcgcagggaa ttgctgcagt tttgtcattt atcgtgatgt ttaagaagta tgatttgctg
     2701 cgcaccagcc gtgaggatta ttacatggat tggaacggaa tctggcgcat gctctcaatc
     2761 ggtctgcaga tggcactcaa ctattcaatc actgctatcg gaacgatgat cttccaggct
     2821 gcggtcaatg tgttcggttc gcaggtggta gctgcgtata ccgccgcatc caaggtcagc
     2881 aatatcgcga cacagaccat gcctacactt ggaacagccg ctgcgactta ctgcggacaa
     2941 aatcttggag caggaagata cgacaggata tttaagggta tgaagtcatg ctttttcata
     3001 tgtataggct gcgcagtttt agcggcagca attaactgct ttgtcggacc gtacatgata
     3061 ggctggttta tcacatcgcc tacggcaact gacattgact gtgctatgaa atatctgaaa
     3121 atcgcgagtg tcttcatgct tcctcttgca tggatttttg catacagaaa cggtctgcag
     3181 ggacttaaca agggtcttgt gcctatgctt agcggtgcga tggagcttgt gtcgagatgg
     3241 attgcaatac ttgccctgtc aaagccattt ggttacggcg gtgtgtgctt tgcagatccg
     3301 gcagcctggc ttacaacagg aatactactt attgtgacct actatgtgtg ggagatgcgc
     3361 atgagaaaga agatacagat aaaatagatt tcacacaaat tggtttattc gtggtacaat
     3421 taagccacgg ataaatcaat ttcatttaca gagataaaga ggcggattgc atgaattatt
     3481 atgagctttc aaacactgtc acccctgata ccattggata caaaaatggc ttatggcaga
     3541 aaagatatgt gcagatttac agagtattga ctgttgtatg gagtgttttg acattatgct
     3601 tgttgtttgg aatgttccat agggatgact atagttcagg aatgattaaa tcatgtttat
     3661 tgctcttttt tgcaggtatt atttttctgg tgttgatgct gatcgctgtt gttaatatat
     3721 cagcgaaaag gacagagaat tggagccttc aggacaggca tgattacaat ttggctatgt
     3781 acagaaccag atacaggaat aataggcagc ttcagtcagt tgtgttgata gtgatggcta
     3841 agcagcagct tcttatgtca aactatgatc tggccgcaca ggctcttgca atggttgata
     3901 tcaattgtgt gaagctgcca tatttaagag attattattt ttgcaatgct gcagttttgt
     3961 tcctatgtga taagccgggc tggcaggagt ggctggataa gtgctatgct gtgccggcga
     4021 atcaaaagca gatgacggac atgcagattg gtgcattgtt tttatcagaa aatgcaaaaa
     4081 tggatttgtg tcaggcaatt tacgcagaca ccaggataaa gcataagtgg ccgacagcaa
     4141 ttgtaattac tgcgatattg gtgttgtatg caggaatttt ctatggagta ggaggcttac
     4201 tgtctcgagg atatcactat agatactggt tcgagctgtc gtctgttctg ataacatacg
     4261 caggctgtat ggtcttgtcg ctttactgga taatcaggtg gatattattc agtgacagcg
     4321 tatctaaaaa gtcagaaggg gtaaggctat tcagaaaagt aatactcata gtcatgtggc
     4381 tgtgtttgat ggctgccatg gttattaatc tgataatttc attcatcggc atggatacgg
     4441 agaaaaaggt atatgcgaat ggtcttatct gcatgagtac ttcggatgga tatagtactg
     4501 atgaatatta taacatcaag gttggacctt tcctgagacg aaatttgaca tttaaggaaa
     4561 caataaagtt tggacttata aacgatgatg acagcaccgg agacagtgga aatgatgctg
     4621 gtgatagtgg tgcaaatgac agtgaaaaca gcactgagaa taattcacag tcatacaatg
     4681 acgcttctga aaatacagaa aatgagaatg caggaaatga gaacgctgac gaaagcctgg
     4741 atacagaaag catggccgta tatgattaca tgtcagctgc agggatactc tcagccgggg
     4801 atgccggtat tattcgcaac ggttccacag ctaaaggtac tttttattct gttttcgaaa
     4861 ccggcgaggc tgatggaaat acgtatgaga ataggttagt ttatgacaga gtttccgaaa
     4921 atggacagtg tgatttgttt gtttatgagc agttggttag tggctcagat acaaggctac
     4981 tgggatttta tgctgtaaat aaaaccacaa atgaggtttt ctcagcggat agaaactcat
     5041 ggggtggtgt gagcagcagg gaatatcagg acgcaacagg agaaaattag gctggtacat
     5101 gtcacataaa cgatgtacta gctggttgtc tccctgcgaa tcagcacagg cgctatcttt
     5161 ttgtgcgttg gcgctaaatc gggctcaggc tttctttgtt tgcggttatc catctgctta
     5221 acgagtaaat ccatggcggt tcttgcgatt acatctatct gctggccgat ggtggtgatt
     5281 ggaattatgg cttcgttggc aattggtgaa ttgtcgaagc cgactatctc atagctgtcc
     5341 ggcaggcagc cgtatttttg tatgacaaga ttcaaaaaca tgttggcata tgtgtcgttt
     5401 gccaggaata cccctttctt tttatcagga tatttctcat ctatattgtt aaaaatcacc
     5461 ttcatctgag aaaataaatc gttgtacgaa tcaccggata cgttcagact gagctcatag
     5521 ggaatattgt gctcggtaca ggtgtcctca aacgcacgga ttctgtcgta agccgggaca
     5581 tactttggta catctacatt gacatggatg aggatatcac atttgtcacg tattaaaaga
     5641 gatgtggcct gaagcgcacc catatagttg tcggaggtga cactgcttat atgcatggat
     5701 tctcgctcga ttgcgacaat tggaatgtgg tatgaagcaa gcttttcaga tgagagagtg
     5761 tgacttagca cgataagacc ctctatctga taagctaaaa gttcatcaat gtagttttct
     5821 tcctcagccg cccccttgtc actcataaag atgagaaact tatagtgata gtcagtgtag
     5881 gtggctaaaa gctgtgtgag catctcagag tagtagtgca ggtaaaggtt tggcactatt
     5941 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnngttg tttttgaaaa gtgtgtatat tccgcaatat
     6121 ctgcaaatgt aacctgtttt ttttccatgg gatgtctcct tataaaaatt acttatatta
     6181 caaatgttta taaacagtat acagcgcaat ttccaaaata tcaacaaaaa agtaaccggt
     6241 tacttttttt gagaaaagta ttgacacagc acaaatgata ttgtattatc aaattataaa
     6301 gtaaccggtt actttaccgg ttacacaaga gcgtaaatta agtaaaaaag acaaaatgtg
     6361 aaagagagtg gaggtcacag caaatgaaag aaaaaaagca gtcattgaaa agcaaagtga
     6421 catatgtgaa gaaaaactgg atgttgtaca tatttttcct tatgccggca cttttactga
     6481 caattatatt caagtatgtt ccgatgggcg gactgctcat agcattcaag gattacaatg
     6541 tcataaaggg tgtgctgggg agtccatggg tcgggcttga atatttcaag agatttttat
     6601 catcacctga ttttatgaat taccttatga atacgttaaa ggtaagtatt tacggattgt
     6661 tatggggatt tccggtaccg attatacttg cactgcttct gaatcgtatt cgcaaagagg
     6721 gcatcaagaa aaagatacag cttctgattt acgcaccgaa ttttatctca gttattgtac
     6781 tttgtggtat ggttcgtatg tttttatcac caatcggacc aatgaataag atactcggca
     6841 tcagcacaaa ctggatgaca atgccggctg cattcaggac aatctacata gcgagcggca
     6901 tctggcaggg agcaggctgg gcttcaatca tgtacacagc ggcattatcc aatgcgagca
     6961 aggagcttga ggaggcagcg agaatggacg gcgcaaacat cttccagcag atgtggtatg
     7021 ttgagcttcc ggctattaag aatattatag tgatacagtt catcctgcag gcaggaaata
     7081 ttatgagtat cggtttcgag aaagcctatg cgttacagac agatatgaac ttaccggcat
     7141 cggaaattct ttctacatat gtatacagaa tcggactttt gaatggagac tatggttatt
     7201 caacagcggt tggtcttttc aactcggtaa tcaacgtaat attgctgatt atagtcaatg
     7261 cggttgtaaa gaagctaaac gatggtgaag gaatctagga ggaaagtgca atggaaaaag
     7321 atatgacgat aaaaatcaaa tattcacgga ctgataaaat tgttcttgga attggatata
     7381 cacttcttgg gttgtttgtg cttgcaataa ttgttccgtt agtatatgta gtgctggcat
     7441 ctttcatgga tccgacagta cttaacaatc agggactgag cttcaacata aaggactgga
     7501 ctattgatgc atacagacga gttctggaaa atgacatgat atggcgcgga ttttttaatt
     7561 cgttttttta ctcaatagcg tttgcggcaa tctcagtatt tgtcacaatc ctggcagcat
     7621 atcctatgtc taaaaaggaa tttgtcggaa gaaaattttt caatgtgatt tttattatca
     7681 caatgttttt tggaggagga atgattccta cattcatcct tataaatcag ctccatatgg
     7741 taaacaccgt atgggcaatc ctcattccgg gcgctttcaa tgtgtggaat atgattttgg
     7801 cgagaaccta ttatcagtca gtgccaaatg agcttcgtga ggcatcagcc atcgacggag
     7861 caaatgaaat acagcatttc tttaagatta tgcttccggt ttgtaagccg attattgcgg
     7921 tgcttatgct ttggtcattt gtaggaatgt ggaacagcta ctttgatgca atgatttacc
     7981 tgaatgacgc aaatctccag ccattacagc ttgtactgcg ttcaattctc gtgcagaata
     8041 cacctcagcc gggcatgatt gcagatatcc agagtacagc tgagatggca aaggttgcag
     8101 agcagctcaa gtacgctaca attgttgtat caagtttacc acttttgatt atgtatccat
     8161 ttttccagaa atattttgat aaaggaatca tggttggatc tgttaagggc taacagaaag
     8221 gatgagtatg gatatgaaaa aaagaagctt caaaaaatta attacacttt cgcttacact
     8281 tgcaatgctg acaggtgtgg caggctgtgg aaagcagcag agcttaaatg ctgagacaaa
     8341 tccggacaca ccggtatcag atgtgcagtt ccctctgaag gagaaggctg agctgtcatt
     8401 tatcacaagt gcaccggcca catccacaca ggatcctaat gagaggacta tcttcaagag
     8461 aatggagaag cagaccaacg tgcatatcga ctggacatgc ttcgtttcag accagttctc
     8521 tgacaagaag aatctggcat tggcgcagtt tggtaatctg ccggatggac tgtttaatgc
     8581 aggtatgagt gattacgacc tgctcagata tgcaaagcag ggtatcatta ttccggtaga
     8641 gaatctgatc gataaatata tgcctaatct tcaggc
//
LOCUS       DPUF01000167           11851 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8652, whole
            genome shotgun sequence.
ACCESSION   DPUF01000167 DPUF01000000
VERSION     DPUF01000167.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11851)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8652"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            1..813
                     /locus_tag="DHV81_05960"
                     /pseudo
     CDS             1..813
                     /locus_tag="DHV81_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569469.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start and stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
     gene            908..2747
                     /locus_tag="DHV81_05965"
                     /pseudo
     CDS             908..2747
                     /locus_tag="DHV81_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516659.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
     assembly_gap    1903..1962
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2748..2860
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2960..3508
                     /gene="spo0A"
                     /locus_tag="DHV81_05970"
     CDS             2960..3508
                     /gene="spo0A"
                     /locus_tag="DHV81_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HCI94013.1"
                     /translation="MSTLNVAVADEKKNMQSLLKGMEFLGTARSSDEAYNMINGNAGS
                     DVVLVYVKRASGDTEPLDDTYMEKKSLETQVTDIIHEIGVPAHIKGYQYLRTAIVMCV
                     EDMEMLSSITKLLYPTIAKKYKTTSSRVERAIRHAIEVAWSRGRMDTIDSMFGYTVNY
                     GKGKPTNSEFIALITDKIRLGM"
     gene            3677..5128
                     /locus_tag="DHV81_05975"
     CDS             3677..5128
                     /locus_tag="DHV81_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCI94014.1"
                     /translation="MDKMKILMAASEAIPYMKTGGLADVVGSLPKYFDKDRYDVRVIL
                     PKYKCMDDKLLPQLKFVCHFYVNLNWRRQYVGIFTSQYDGVTYYFVDNEFYFAGDKPY
                     NNIYEDVEKFAFFSKAVLEALPVIDFAPDVIHCNDWQTGLLPVFLKTVYGSDNFYAGI
                     KTVFTIHNMKFQGRWKIKEVADVTGLPEHIFNSGELEFYGEANYLKGGIVYADEITTV
                     SPTYADEICTPEGGEGLDGLMLERRRHLRGIVNGIDYDVFNPMKDTYLDKHFSVRELS
                     GKVVNKRQLQEKYGLAVDKDVMLIGIVSRLTKQKGFDLVAYVMEEMLSTMNVQFIVQG
                     TGEQQYEEMFNYFHGRYPQKLGVYIGYSEENAHEIYAGCDAFLMPSLFEPCGLSQLMS
                     MRYGTLPIVRETGGLKDTVVPYNEYENTGDGFSFANYNAHDMLHVIKYALDVFENHKD
                     RWQEMMQRAMRHDFSWKASAKEYEKLYDMLLGN"
     assembly_gap    5261..5393
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5394..5626)
                     /locus_tag="DHV81_05980"
                     /pseudo
     CDS             complement(5394..5626)
                     /locus_tag="DHV81_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743004.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start and stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    5789..5982
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5983..6542)
                     /locus_tag="DHV81_05985"
     CDS             complement(<5983..6542)
                     /locus_tag="DHV81_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94015.1"
                     /translation="MKYTEFLHTIETEITARLDNNFKLTIHPVKKNNGMIYDGLVIIN
                     PKFNIAPTIYLNPYYHWYLDGVSIDSILDAILSTYNDFKPTEDFDISFCDDFSKVKEK
                     IIFQLISYDKNQKQLKDVPYIKYLDFAIIFEVYVGAQLPEFGTITVTDALLSRWGIDT
                     ETLIRASMQNTPRILPCSIINMAEFLA"
     gene            complement(6721..8595)
                     /locus_tag="DHV81_05990"
     CDS             complement(6721..8595)
                     /locus_tag="DHV81_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     permease/substrate-binding protein PotCD"
                     /protein_id="HCI94016.1"
                     /translation="MVKKIASRVYMGLIFLFLYLPIVVLIVLSFNNSKSKVKWGGFTL
                     DWYIKCFQSERIMSAFSTTLQITLLAAVISTIIGTLAAMGISAMKKRNQTIYLGATNI
                     PMLNADIVTGISMMLLFVKFMNLGFVTVLIAHITFNIPYVILNVLPKLKQTNKYTYEA
                     ALDLGASPLYAFFKVTWPEISPGVFSGFMMAVTMSLDDFSITYFTKGAGVNTLSTMLY
                     TELKKGVKPELYALSTILFFTVLLLLVVVNINSNQIPVSASKKPARAKKLRIVKRSVS
                     IAIVAVLVIGCFSVFTISAGSTNNDNAITVLNYGKYIDESVIDSFEQETGITIKYEEY
                     EEPEEMYTKYKSGAIDYDVICTSDYIIEKLISEGEVNKIDYSSMPNYQNVNQDIIDMS
                     ASFDPTHEYTVPYFYGTLGILYNKKMADASDLNTWDCLWNGKYKDNMIMINSVRDAFT
                     PALRTLGYSINETDTAKLDEAFDILNKQSSDVLAYYVDETCDEMVAENAAIAVCYSGE
                     AAAAMAENDNLDYIVPKEGSNLWIDSWFIPKTCKNKEGAQEFLNYICSDEPAQLNFEY
                     VYYASPIQSVIDNQDAETKENEAINPPSDMLKNCEVYRALNDDDATLYNTLWQRLKSD
                     "
     gene            complement(8589..>8795)
                     /locus_tag="DHV81_05995"
     CDS             complement(8589..>8795)
                     /locus_tag="DHV81_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI94017.1"
                     /translation="PSLTSFAISQILGGGKVLLIGNIIEQDFVHGSQWGAGSGLSLVL
                     MVFVLISMALVNLFDKEGDQSALW"
     assembly_gap    8796..8905
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8906..9426)
                     /locus_tag="DHV81_06000"
     CDS             complement(<8906..9426)
                     /locus_tag="DHV81_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI94018.1"
                     /translation="MSKIKKQLLAGPYLIWIIGFIILPLFMIVYYAFKGSDGGFTFEY
                     VAAIAQHTNIKALLLSLRLGIICTIICLVLSYPLAMILNGLKIKNQSFVVFVFMLPMW
                     MNFMLRILAWKQLLSKNGVINSILTTLGLPGFNIMNTSGAVVLGMVYDFLPFMLLPIY
                     NSMTRIKNDWIEAA"
     gene            complement(9419..10492)
                     /locus_tag="DHV81_06005"
     CDS             complement(9419..10492)
                     /locus_tag="DHV81_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HCI94019.1"
                     /translation="MGKKLIDFIDITKSYNGNVVLDDLNLYIRENEFLTLLGPSGCGK
                     TTTLRMLGGFETPDKGKIMFDSKDITNMPANERNLNTVFQKYSLFPHMTIAENIAFGL
                     KIKKKSKAYIDDKIKYALKLVNLDGFENRSVDSLSGGQQQRIAIARAIVNEPKVLLLD
                     EPLGALDLKLRQSMQYELIRFKNELGITFVYVTHDQEEALTMSDTIVVMNQGYIQQIG
                     TPEDIYNEPENAFVADFIGHSNIIDGVMIKDKLVEILGVKMPCVDEGFGENTPVDIVI
                     RPEDVELVPAEDGYMQGDVVSNIFKGVHYELEVKANGYDWMVHTTKYVEVGSHVGIRV
                     IPFNIQVMHKPESSDEKAVEIDV"
     gene            complement(10621..11160)
                     /locus_tag="DHV81_06010"
     CDS             complement(10621..11160)
                     /locus_tag="DHV81_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCI94020.1"
                     /translation="MDIGHRMKELRIQYGLTQQELADRAELTKGFISQLERNQNSPSV
                     GTLLDIIQCLGMTPAEFFTDSEPEQIVYKANDYFEKIDEEKNSKVEWIIPNAQTRSME
                     PVRLTLHPGGSSEIYLPQECEEFGYVIKGTVKLCYGGKVHTVKAGESFYFKAGKKHFI
                     ENKSSKDAMLIWVSNPPSF"
     assembly_gap    11254..11403
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11532..>11851
                     /locus_tag="DHV81_06015"
     CDS             11532..>11851
                     /locus_tag="DHV81_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI94021.1"
                     /translation="MKLMQNSGVPIYQQIADSFKTDILAGRYEQGEFLPSIRGLAKDL
                     KISVITTMKAYEQLADEGLITAAQGKGFYVNAQDSEMIKEQHLRKVEESLTDAIAAAE
                     VAGIS"
BASE COUNT         3094 a         2239 c         2649 g         3107 t
ORIGIN      
        1 gaggttataa ggacaaacag caccgtaaat gattcaaagg agcctgttac tgaggctgat
       61 catagaggtg ttatccgttt caaccatgtg gatttcagat atccgggagc aaaggaggat
      121 gtgctttcag acattgattt tgtggcggag ccgggcaaaa tcacagctat catcggaagt
      181 accggctgtg gaaagtctac acttgtaaac ctgattccgc gtttctatga cgtgacggga
      241 ggctcaatag agcttgatgg acacgatatc agagactaca cactaagcga gcttcgcgag
      301 tcaataggct ttgttccgca gaagggtatt cttttttcgg gaaccatcgc ctcaaacctg
      361 cgctttggta aagcagacgc gagtgatgag cagataaaaa aggctgcgga tatcgcacag
      421 gcttctgagt ttatcgagac aaagaatgac aggtatgaaa gctcaatcgc acagggcggc
      481 tcaaacgtgt caggaggcca gaagcagagg cttgccatag caagagctat cgcaaaggat
      541 cctcatatct atgtgtttga tgacagcttt tcagcactcg atatgaagac tgatgcgcgt
      601 cttcgtgcag ctttagcaga ggttacagag aatgcttcag tcattattgt ggcacagcgt
      661 atcagcacga ttattcatgc agaccagatt ctcgtgctcg atgacggtaa aatcgtagga
      721 aagggcaccc acgaggagct tcttaaaaat tgcgatgtat atatgcagat tgcacagtca
      781 cagctttctg caaaggagct tggacttgat gataaggtgg cagaaacagc aacagatact
      841 gcaacaaaaa cagcaacaga tacttcggct gtatcaggct tagataatga aaaggagggc
      901 atgtaagatg agtgagaata ataacgaaca gttcaagatg cctcctaaga ggccaagagg
      961 gccaatgggc agaggccccg gaatggtcac agaaaaggcc aaggatttca agagctctac
     1021 aaagaagctc ataaagtatc tcggcagata ctgggctgcc atcattgcag ttatgatatt
     1081 tgcagcagtt tcgactgtct tctccgttgc aggaccgaag gtcatgggta aagccaccac
     1141 tgcgcttgcc gagggactta tgaacaagat tgcaggaaca ggcggcatag attttgatta
     1201 tattgcaaag gttcttttgt ttacgctggc tttatatgtg gtgagtgcca tctttatgtt
     1261 tgcccagggg cttatcatga caggcatcac gcagaagacc tgctacagga tgcgtaagga
     1321 catcacgagc aaaatcaacc gtatgccgat gaagtatttc gagtcaagga catacggtga
     1381 ggtactttcg cgtatcacaa acgatgtgga cacactcgga cagagcttga accagagtat
     1441 cacacagatt attacatctg ttgccacttt ggtcggtaca ctggtcatga tgctgtctat
     1501 ttcaccgctc atgacgctga tttcactcgt tatcctgcca gtttcgatga tacttatttc
     1561 gtttgtcata aagcattcac agaaatactt cagacagcag caggagtatc tgggacacat
     1621 aaacggacag gtcgaggagg tgtacggagg ccacctcgtc ataaaggcat ataataaaga
     1681 ggcagagact gtaaagacct ttaaggaggc caacaatgtg ctctacaaat ccgcatggaa
     1741 atcacagttc ctttcaggac ttatgatgcc tatcatgcag tttgtaggaa acttaggata
     1801 tgcgggagta gccatctcag gtggtatact tgccataaag aatgtcatca caatcggtga
     1861 tatccaggca tttatacagt atgtgaagaa tttcacacag ccnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngagcgtgt atttgagttc
     1981 ctcgaggagg aggaagagga tataacagtg gaaaatcctg taaagccaga ccatatcgag
     2041 ggaagtgtag agttttcgca tgtacacttt ggttataatc ctgaccagat catcatcaat
     2101 gatttttctg caaaggtaaa gccgggacag caggttgcta tcgttggacc gacaggagcg
     2161 ggaaagacta ccatggtcaa gcttcttatg agattttatg atgtaagctc aggtgccatt
     2221 cttgtagacg gacatgatat cagggattac aacagagccg atctgcgaga tgcattcggc
     2281 atggtattgc aggacacatg gctctttaag ggcaccatta tggaaaatat ccgctacggc
     2341 cgtctggatg ccacagatga ggaggtcatc gcggcagcaa aggcagcaca tgcgcatcac
     2401 tttatccaga cattgccggg tggctacgat atggagctca acgaggatgc atccaatgtg
     2461 tcacagggac aaaagcagct tttgacaata gcaagagcca ttcttgcaga caatccaatc
     2521 cttatccttg atgaggccac atcctctgtc gatacacgta cagaaatccg tatccagaag
     2581 gcactcgaca atctgatgaa gggcagaaca agctttgtca tcgcccacag gctgtctact
     2641 atcaagaatg ccgatatgat tcttgtcatg aaggacggag acattatcga gcagggtaca
     2701 cacgatgagc ttcttgcaaa gaacggattt tacgcagagc tgtataannn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaaaaaata cgctctcgac
     2881 aaaattcgac aaaatgtgtt atcataaaag catacaattt gttttgacat gacagtaatt
     2941 ggcggcatag gagagtttga tgagcacttt aaatgtcgca gtagcagacg aaaagaaaaa
     3001 catgcagagt ttattaaagg gcatggagtt tttaggaaca gcaagaagta gtgatgaagc
     3061 atacaacatg ataaacggta atgccggttc tgatgtggta cttgtgtatg taaagagagc
     3121 atccggtgac actgagccac ttgatgatac atatatggag aaaaaatccc ttgagacaca
     3181 ggtgacggat atcattcatg agataggtgt tccggctcat ataaagggat atcagtatct
     3241 taggactgcg atagtgatgt gcgttgagga tatggaaatg cttagctcta tcacaaagct
     3301 tttgtatccc actattgcaa agaagtacaa gactacctca agcagggtgg agagagccat
     3361 aaggcatgct atcgaggtgg catggagcag aggcaggatg gatacgatag actccatgtt
     3421 tggatacact gtaaactatg gtaagggcaa gcctaccaat tcggagttta ttgccctgat
     3481 tacagacaaa atacgtctag ggatgtaaaa atcgtcatta tagacaaata aaaccacgtt
     3541 ttgataaatt tactaggcaa aatgtactcc cgcgtgatat aatatagtat acattctatt
     3601 ggtttcagga tgtatactat attttttatc agattaatcg ttgtttaaat aactgtaatc
     3661 tgggggaggt ttcagcatgg acaaaatgaa aatattaatg gccgcatcag aggcaatacc
     3721 ttatatgaag acaggtggac tagctgacgt agtgggatct ttaccgaaat attttgacaa
     3781 ggacagatat gacgtgagag ttatacttcc gaagtacaaa tgtatggatg ataaattact
     3841 gccacagctt aagttcgtct gtcattttta tgtcaatctc aactggcgca ggcagtatgt
     3901 ggggattttt acatcgcagt atgatggtgt gacatattat tttgtcgaca atgagtttta
     3961 ctttgcagga gataagccat acaacaatat atacgaggat gtggagaagt ttgctttctt
     4021 ttcaaaggcg gtgcttgagg ctttgccggt tatagatttt gccccggatg tgatacattg
     4081 taatgactgg cagacaggac tcttgccggt ttttctaaag acagtatacg gctcagacaa
     4141 tttctatgcc ggtatcaaga cagtctttac cattcacaat atgaagtttc aagggcgatg
     4201 gaagattaaa gaggtggctg atgttacagg acttccggag catattttta attcagggga
     4261 gctggagttc tatggtgagg ctaattattt aaagggcggc atagtatatg cagatgagat
     4321 tacgactgtc agtccgacat atgcagatga aatctgtaca cctgagggcg gagagggcct
     4381 ggatggcctg atgcttgagc ggcgcaggca tttgagaggc attgtaaacg gtatagatta
     4441 tgatgtcttt aatcctatga aggatacata tctggataag catttttctg taagggaatt
     4501 gagtggaaag gtcgttaata agcgtcagct gcaggagaag tatggtctgg ctgtggataa
     4561 ggatgtcatg ctgattggaa tagtttcgcg tctgacaaag cagaagggct ttgatctggt
     4621 ggcatatgtg atggaggaga tgctttccac catgaatgta cagtttatag tgcagggcac
     4681 aggagagcag cagtacgagg agatgtttaa ttatttccat ggcaggtatc cgcagaagct
     4741 tggagtatat atcggttact cagaggaaaa tgcacatgag atatatgcag gctgtgacgc
     4801 atttctcatg ccgtctcttt ttgagccgtg cggcttgagc cagctcatgt ctatgcgata
     4861 tggtacgctg cctatagtca gggagaccgg tggccttaag gacactgtag tgccgtacaa
     4921 tgagtacgag aatacgggcg atggcttttc ctttgccaat tataatgcgc atgatatgct
     4981 gcatgtcatc aaatatgctc ttgatgtgtt tgaaaaccat aaggacagat ggcaggagat
     5041 gatgcagcgc gccatgaggc atgatttctc atggaaggca tctgcgaaag agtatgagaa
     5101 gctttacgac atgctgcttg gcaactagta catcgctctg caaataacag gccaataatt
     5161 ttcatgaatt tccaactatg ttcttagaaa gtaaaatacc ggatacatat ctatgatatg
     5221 atatccggta ttttgtgtta tcgggttata tgattatcag nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctatcca
     5401 gcggcattac cagaaattca tggatactgc ttggtatgag cagcatattg ccgccgagct
     5461 tttttgccag cgagcttagc acaccagggt agagtatggc ggatgcacca tttgtctgcc
     5521 tgctgtttgt aaggatatac ataggcaggc tgtcaaaatc acagactgct cgcatctgat
     5581 tggcatagcc cggacatttt tttgcaagaa actctgccat gttgatnngg ttatatgatt
     5641 atcagtagac agtgtctgtt ttactatcgt atatgtagta cctctcgccg agtacttcct
     5701 cgtcgcgtac ctctgtagag tttacctcac agatgaattc gcttaggttg cagacatcta
     5761 tatcgctatc cagcggcatt accagaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcaagaaa ctctgccatg
     6001 ttgattattg agcatggaag tatccttggg gtattttgca ttgaggcgcg gattagtgtt
     6061 tctgtatcta tgccccagcg tgacagaagc gcatctgtga ctgtaatggt gccaaattca
     6121 ggcagttgcg ctcctacata tacttcaaat atgatggcaa aatccagata tttgatatag
     6181 ggcacgtcct ttagctgctt ttggtttttg tcgtagctta tgagctggaa aattattttc
     6241 tccttcacct tggaaaaatc atcacagaag cttatgtcaa aatcttcagt tggcttgaaa
     6301 tcattgtagg tggataaaat tgcatcgaga atagaatcaa tggatacacc atcaagatac
     6361 caatggtagt aaggattgag ataaatagtc ggtgctatat tgaattttgg attgataatc
     6421 accaatccgt cgtagatcat cccattgttc tttttgacag gatggatggt aagcttgaaa
     6481 ttattgtcaa gtcttgcggt tatttctgtt tctatagtat gtaaaaactc cgtgtatttc
     6541 atgaatctcc ttgcggagca taaaaatatc ggatctcgtg gaaaagtaaa acccacgaag
     6601 cagggcgctt cgtgggtttt actctatcat tgtttgttga agtttgcaat atgtattttt
     6661 gtacaatttt tagacttcat ttttgtgata tcatacaaaa tacagctgcg caatccattt
     6721 ttaatctgat ttgagcctct gccacagtgt gttgtaaagt gttgcgtcat catcatttaa
     6781 agctctgtat acttcacagt ttttgagcat atcggaaggc ggattaatcg cctcattttc
     6841 cttcgtttct gcatcctggt tgtcgatgac agactggata ggtgatgcat agtatacata
     6901 ttcaaagttg agttgtgccg gctcatcact gcagatataa ttcaaaaatt cctgtgcgcc
     6961 ctctttattc ttgcatgtct ttggaataaa ccatgagtca atccagaggt ttgagccctc
     7021 ctttggcacg atatagtcca ggttatcgtt ttcagccatg gctgcagctg cctcaccgga
     7081 gtagcacact gcgattgccg cattttcagc aaccatctca tcacaggttt catccacgta
     7141 gtaggcgagc acatcactgc tttgtttgtt tagtatatca aatgcttcat cgagctttgc
     7201 tgtatctgtc tcgtttatcg agtagccaag tgtgcgaagg gcgggagtaa aggcatcacg
     7261 gacagaatta atcataatca tgttgtcctt gtatttgccg ttccacaggc agtcccaggt
     7321 atttaggtct gatgcatcag ccatcttttt attgtatagt ataccgagtg tgccgtagaa
     7381 ataaggcaca gtgtattcat gtgtcgggtc aaaggatgcg gacatgtcga ttatatcctg
     7441 atttacattt tgatagtttg gcattgagct gtagtctatc ttgttgacct caccctcact
     7501 tatgagcttc tcgatgatgt agtcagaggt gcagatgaca tcgtaatcga tggcaccgga
     7561 cttgtacttt gtgtacatct cctcaggctc ctcgtattcc tcgtatttaa tggtgattcc
     7621 ggtttcctgc tcgaagctgt cgattactga ttcatcgatg tatttaccgt agtttagcac
     7681 tgttatggca ttgtcattgt tcgtgcttcc ggcgcttatg gtaaataccg aaaagcagcc
     7741 gattacgagt actgcaacaa ttgcgatgct cacactacgc tttacgatgc gcagcttttt
     7801 agcccttgca ggcttctttg aggcgctgac aggtatctgg tttgagttga tgtttaccac
     7861 aaccaaaagc aacagcactg tgaagaaaag tatggttgac agagcataca gctccggctt
     7921 tacacccttt ttcagctctg tgtagagcat ggttgagagt gtgtttactc cggcaccctt
     7981 cgtaaagtag gtgatggaga agtcatcgag tgacattgtg accgccatca taaagccgga
     8041 gaacactccg gggcttattt caggccaggt gaccttgaaa aatgcatata gaggtgatgc
     8101 gcccaggtca agtgcggcct cataggtgta tttgttggtc tgcttgagct ttggaagcac
     8161 attgagtatc acataaggga tattgaacgt gatgtgtgcg ataagcactg tcacaaagcc
     8221 cagattcatg aattttacaa acaaaagcat cattgagata ccggtcacga tatctgcatt
     8281 gagcatcggg atgtttgtag cgcccagata gattgtctgg ttacgtttct tcatggcgct
     8341 tatacccatg gctgcgagag ttcctattat tgtagaaatc acagctgcaa gcagcgtaat
     8401 ctgcagtgtt gtagaaaatg ccgacataat ccgctcgctc tgaaagcact ttatgtacca
     8461 gtcgagcgtg aagcctcccc atttaacctt ggatttggag ttgttgaagg agagtactat
     8521 taatacaacg ataggtagat acagaaacaa aaaaatcagt cccatatata ctctggatgc
     8581 tatttttttt accataatgc gctctggtct ccttccttat caaacagatt gacgagtgcc
     8641 atactgatta atacgaatac catgagcacg agtgagagac cgcttcctgc gccccactgc
     8701 gagccatgga cgaaatcctg ttctatgata tttccgatga gcaggacctt gccgccaccg
     8761 agtatctgtg agatggcaaa ggatgtgagt gatggnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnngccgc ctcaatccag tcgtttttga ttctggtcat
     8941 ggagttgtat ataggcagca gcatgaatgg cagaaagtca tagaccatgc caagcacgac
     9001 tgctccggag gtattcatga tgttgaagcc cggcagtccg agagtggtga gtatcgagtt
     9061 gatgacaccg tttttggaca aaagctgctt ccatgcgagt attcgaagca taaagttcat
     9121 ccacatagga agcatgaaaa caaataccac aaagctttgg tttttaatct ttaagccgtt
     9181 taaaatcatc gcaagtggat aggacagcac caggcatatt atggtgcata tgattccaag
     9241 ccgtagggat aaaagcagtg ccttgatatt ggtgtgctgt gctattgcag ccacatattc
     9301 aaatgtgaag cctccgtcag agcctttaaa cgcgtagtac acaatcatga aaagcggaag
     9361 tatgataaat ccgataatcc atatgaggta cggccctgcg agcagctgtt tctttatttt
     9421 agacatctat ctccaccgcc ttttcatctg aggactcagg cttgtgcatt acctggatat
     9481 taaatggaat caccctgata cccacatgac tgccgacctc tacatatttg gtggtgtgca
     9541 ccatccagtc gtagccgtta gcctttacct caagctcata gtgcactccc ttgaagatat
     9601 ttgacacgac atcgccctgc atgtagccgt cctccgcagg caccagctcc acatcctccg
     9661 ggcggatgac tatatcgaca ggcgtgttct caccaaagcc ctcgtccaca caaggcatct
     9721 ttacaccgag tatctcgaca agcttatcct ttatcataac accgtcgatg atattactgt
     9781 gcccgataaa gtcagcgaca aaggcatttt caggctcatt gtaaatgtcc tcaggtgtgc
     9841 caatctgctg gatatagccc tgattcatga ctacgatggt gtcagacatg gtaagcgcct
     9901 cctcctggtc gtgtgtgacg tagacgaagg tgataccgag ctcatttttg aagcggataa
     9961 gctcatactg catgctctgg cgcagcttta agtcgagagc gccaaggggc tcgtctaaca
    10021 gcagtacctt aggctcgttt acaatagctc ttgcaatagc gatacgctgc tgctggcctc
    10081 cgctaagaga atctacagag cggttttcaa agccgtcgag attgacgagc tttaatgcat
    10141 actttatttt gtcatctatg taagccttgc tttttttctt tattttaaga ccaaacgcga
    10201 tgttttcagc tattgtcata tgagggaaaa gagagtattt ctggaaaaca gtgttcagat
    10261 ttctctcgtt tgcaggcata tttgtgatgt cctttgaatc aaacatgatt tttcccttgt
    10321 caggtgtctc aaagccaccg agcatgcgaa gtgtggtggt ctttccgcag cctgatggtc
    10381 caagcagcgt cagaaactca ttctccctga tgtatagatt taaatcgtcg agtacaacat
    10441 tgccgttgta tgactttgtg atatcgataa aatcaattaa tttctttccc atggtgtaaa
    10501 cctttctcat gtaattatag tactggtagg aactgtatgt ttataatttg ctgctgtaca
    10561 cactaaaaat gtggccggca gtaattacag ccatataaaa gagcttctgc aggtgaagcg
    10621 tcaaaagctt ggaggattgc tgacccatat gagcatggca tcctttgaag acttattttc
    10681 aataaaatgc ttcttaccgg ctttaaaata aaaggactca ccggccttta cggtgtgcac
    10741 tttgccacca tagcaaagct tcactgtgcc ctttatcaca tatccaaact cctcacattc
    10801 ctgaggcaga tatatttcag agctgccacc cggatgaaga gtgagcctga caggctccat
    10861 ggagcgggtc tgtgcgtttg gaatgatcca ttccaccttg ctgttttttt cctcgtcaat
    10921 tttctcgaaa tagtcgtttg ccttgtatac aatctgctca ggttccgaat ctgtaaaaaa
    10981 ctccgccggt gtcataccca ggcactggat gatatcaagc agggtgccga ctgacggtga
    11041 attctgattt cgctcgagct gtgagataaa tcccttcgtc agctcagccc tgtcagcaag
    11101 ctcctgctgg gtaagtccgt actggatacg aagctccttc atacgatgtc cgatatccat
    11161 gtttacttcc ttttctaata cattttatgt tatatttact gtaggtttaa taatagattt
    11221 tttgtttact aaaaataaac caacgagata attnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnntatcaga tataagagtg tatgtcaaat caaaattttg ggggatgatt tcataaaggt
    11461 attgacaata gaatgtatat agtgtatata taacacatga acagttaata aatacagtac
    11521 ggaggtagca tatgaagctg atgcaaaatt caggagtgcc catctatcag cagatagcgg
    11581 acagcttcaa gacagatatt ttggccggca ggtatgagca gggggagttc cttccgtcta
    11641 taagagggct ggcaaaggac ttgaaaatca gtgtgataac cacgatgaag gcttatgagc
    11701 agcttgcaga tgagggactc atcacagcgg cgcagggcaa gggcttttat gtcaatgcac
    11761 aggacagtga gatgataaag gaacagcacc tgcgcaaggt ggaggagtcg ctgacagacg
    11821 cgatagccgc ggctgaggtg gccggaatct c
//
LOCUS       DPUF01000168            7202 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_39728, whole
            genome shotgun sequence.
ACCESSION   DPUF01000168 DPUF01000000
VERSION     DPUF01000168.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_39728"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="DHV81_06020"
     CDS             complement(<1..980)
                     /locus_tag="DHV81_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCI94022.1"
                     /translation="MKKIVMTGGGTAGHVTPNIALMPALKEAGYDIEYIGSVNGMEKG
                     LIEAQKIPYHGISSGKLRRYFDWKNFTDPFRVLKGYGQAVSLMKKLKPDVVFSKGGFV
                     SVPVVLAAKHCHVPAIIHESDITPGLANKIAIKGAKKVCCNFPETMKYLPADKAVLTG
                     SPIRRELFSGVAENAIKLCNFPDHNKPVILIIGGSLGSKKVNEAVREILPELLKDFYV
                     IHLCGKGNLDNKLAGITGYAQFEYANAELTDMFALADMAISRAGANSICELLALHKPN
                     ILIPLSAAASRGDQVLNAKSFKKQGFSYVIEEEELTKDSLLSAVKEVYSN"
     gene            1234..1500
                     /locus_tag="DHV81_06025"
     CDS             1234..1500
                     /locus_tag="DHV81_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94023.1"
                     /translation="MELSMDNMDIVAGGILAIMLALFMLTVIVTLFALSHRIRLLSNE
                     IYKIEKQVSDYIDELSRSESDKLEIEHKAHQNGVISSVLQEIFP"
     gene            1620..>2186
                     /locus_tag="DHV81_06030"
     CDS             1620..>2186
                     /locus_tag="DHV81_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HCI94024.1"
                     /translation="MKLNKKVIDSITVCGLCAMLTVTAVTNSGRSEVKAEDKVLANTG
                     LEHTGYNSYAGVAATLFDYEKGLTAENAVTVEKENVEMVAAAQDAQPQLTQEQQEWQT
                     KLMPKVEESLNVRAQADEQSDIVGKLYKGSVADIVENDGTWAHIKSGNVDGYVNVSYC
                     VTGTDALSYAYDTCGEIATVNTDGLRVRE"
     assembly_gap    2187..2293
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2294..2854
                     /locus_tag="DHV81_06035"
     CDS             <2294..2854
                     /locus_tag="DHV81_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HCI94025.1"
                     /translation="AVVCDSQTGYIAADYATRSLNTRVGITIEEEQAQIAAQKEAERK
                     AAEEKAAAEAAKAAKKNTSSKKAETTQGEAVSAGADDLTLLAAIIECEAGGESYECQL
                     AVGAAVINRVKSSSYPNSISGVIYQKGQFGPASSGKLARKLSGRISSSCYSAAQEAMS
                     GVDNTGGCTSFNDHGSGISIGNMKFR"
     gene            3090..4079
                     /gene="argF"
                     /locus_tag="DHV81_06040"
     CDS             3090..4079
                     /gene="argF"
                     /locus_tag="DHV81_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCI94026.1"
                     /translation="MQLKGRNFLKLMDYTPEEITYLIDLSQELKEKKKKGIRHDELTG
                     KNIALIFEKTSTRTRCSFEVAAHDLGMHVTYLDPSGSQIGKKESIADTARVLGRMFDG
                     IEYRGYGQEIVEELAKYAGVPVWNGLTNEFHPTQMIADMLTIREHLGRLKGVKFVYMG
                     DARYNMGNSLMVTCAKLGMDFVACTNKKYFPNDELVKYCKDVAETTGASITLTEDVAE
                     GTKDADVIYTDVWVSMGEPEEVWAERINDLKPYQVNAKAFENAKDSAIFMHCLPAFHD
                     LKTTIGKQVYEKFGLSELEVTDEVFESERSVVFDEAENRMHSIKAIMRATLAD"
     gene            4133..5113
                     /locus_tag="DHV81_06045"
     CDS             4133..5113
                     /locus_tag="DHV81_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCI94027.1"
                     /translation="MKEEILRLLRSADGYISGQELCNRFGVSRTAVWKAINQLKEAGY
                     EIEAQQNKGYRLMAAPDLMTEAEIKSLMHTDWVAKEVLYFDTIDSTNIKAQELAEKGY
                     PSGTLVVADKQESGKGRRGRSWVSPSGTGIFMTLMIKPDINPNNASMLTLVAALAVAK
                     AITSVTGEEALIKWPNDIVINGKKVCGILTEMNAQFDYINHIVVGIGINVHNESFPEE
                     ISQMASSLMIEAGGKRFHRAQIIAETMSYFEQYYDTFLKTQDLSALVREYDELLVNRN
                     KSVRVLDPKEPFDGKAMGITPKGELIVDTWESRKLVSSGEVSVRGIYGYV"
     gene            5106..5441
                     /locus_tag="DHV81_06050"
     CDS             5106..5441
                     /locus_tag="DHV81_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94028.1"
                     /translation="MSKEVVLCVSNAYQKKYYLNENFNGLPQTIRDELKIMCVLYTED
                     VGGILELVFDEDGNLDFRTSCKENDFFYDEIGSVLKIKQYRNERRELLESIETYYRIV
                     FLGEGLEEE"
     gene            5445..6227
                     /locus_tag="DHV81_06055"
     CDS             5445..6227
                     /locus_tag="DHV81_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HCI94029.1"
                     /translation="MILALDVGNTNITCGVFDGDRIKASFRITTKMPRTSDEYGMLLS
                     TLLERNQVGMDDIHDAIICSVVPNIMHSLQNGLIKYFNIRPIIVEAGIKTGIRIATPN
                     PQQIGADRIVDAVAAYELYGGPVLVIDFGTATTYDMVDENGTFMGGITAPGIRISAKA
                     LWEDAAKLPEIEIKKPDNILGKDTITSMQAGLVYGQIGQTEYIINKVKEETGMYDAKV
                     VVTGGLGRIISNETENVDVYDPDLTLKGINLVYRKQNRKGVK"
     gene            6232..>6608
                     /locus_tag="DHV81_06060"
     CDS             6232..>6608
                     /locus_tag="DHV81_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCI94030.1"
                     /translation="MDIHQIRKLQIGSVTLPNNLILGPMAGVTDLPFRLLCKEQGAGL
                     LCMEMVSAKAILYKNKNTESLLSIDEREKPVSLQMFGSDPQIMAQIAKQIEERPFDIL
                     DINMGCPVPKIVNNGEGSALMKNP"
     assembly_gap    6609..6737
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6738..>7202
                     /locus_tag="DHV81_06065"
     CDS             <6738..>7202
                     /locus_tag="DHV81_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCI94031.1"
                     /translation="HVAEESGASAVAVHGRTREQYYSGKADWDIIRQVKEAVSIPVIG
                     NGDLLCAEDVIKMQEQTGCDGFMIARGAQGNPWIFSQILHYFETGEHQEKPSFEEVRR
                     MILRHARMMIEFKGEYTGIHEMRKHTAWYTAGYPHSSRLRAAVNTVESLEQLE"
BASE COUNT         2199 a         1426 c         1787 g         1554 t
ORIGIN      
        1 ctgttgctgt acacctcttt tactgcgcta agcagagaat cctttgtgag ctcctcctcc
       61 tcgatgacat atgaaaagcc ctgctttttg aaggattttg catttaatac ctggtctccg
      121 cggctggcag cagcagaaag aggaatcagg atgtttggct tgtgcagagc aagcagctcg
      181 cagatggagt tggctccggc tcttgagata gccatgtcag ccagagcaaa catgtcggtg
      241 agctcagcat tggcgtactc aaactgtgca tagcctgtga taccggcaag cttgttgtcc
      301 agattgccct ttccgcagag gtgaattaca tagaaatcct ttaggagctc tggaagaatt
      361 tcacggacag cttcattgac ctttttagag ccaagacttc ctccgatgat gaggatgaca
      421 ggcttattgt gatcaggaaa attacaaagc tttatggcat tttcggcaac accgctaaaa
      481 agctcgcgtc tgataggtga gccggtgagc acggccttgt cagcaggcag atatttcatt
      541 gtttcaggaa aattacagca cacctttttg gcgcccttta tggcgatttt gttggcaagt
      601 cccggagtga tatctgactc gtggatgata gccggcacat ggcagtgctt tgcggcaagc
      661 acgacaggta cggaaacgaa gccaccctta gaaaatacta catcaggctt gagttttttc
      721 atgaggctta cagcctgacc gtagcccttt aacacgcgga acgggtctgt aaagttcttc
      781 cagtcaaagt atcggcgcag ctttcctgat gaaatgccgt gataaggaat cttttgggct
      841 tcaatcagtc ccttctccat tccgtttact gagccaatgt attcgatatc gtatccggcc
      901 tccttaagtg ccggcataag agctatattt ggagtgacat gacctgcagt accgccgcct
      961 gtcataacta ttttcttcat atatatgtat cctttcttgc attcctgttt gtctcatatt
     1021 tatattatac tcaacacaat aaaagtcaat accaagttac cggtgaaagt ttgctctgca
     1081 atgccaaaca aatgtcgaaa cttgcgaccg ataggaactg ttaagttcaa gtgaattggt
     1141 tataataatc aatagtacat catcctgcgt tataggttaa gttaaatttt gaatgatgta
     1201 tcggaaaaag aatagtaggg aggacacatt aatatggaat tatcaatgga caatatggac
     1261 atcgtggcag gaggcatact tgcgataatg cttgcacttt tcatgctgac agtcattgtg
     1321 accttgttcg cactctcaca caggataaga cttttgagca acgaaattta taaaatagaa
     1381 aaacaggtga gcgactacat agacgagctg tcgagaagcg aaagcgacaa gctggagata
     1441 gagcataagg cacatcagaa tggggtgatt tcatcggtgc ttcaggagat ttttccatga
     1501 aatttgacaa aatgctatta ctgtgatatt ataccgactg taacaaagtt gtaacaaatg
     1561 caacataggt gtaataaacg taacaaaaac aacatatact acaccatatg gaggattata
     1621 tgaagctaaa caaaaaagta atcgatagca ttacggtctg tggactgtgc gccatgctga
     1681 cggttaccgc ggttactaac agcggacgct ctgaggtcaa agcagaggat aaggtgctag
     1741 caaacactgg tttagagcat actggttata atagttatgc tggcgtggca gcgactcttt
     1801 ttgattatga gaagggtctc acagcagaga atgctgttac agtagaaaaa gaaaatgttg
     1861 agatggtggc agcagcacag gatgcacagc cccagctcac acaggagcag caggaatggc
     1921 agacaaagct tatgccaaaa gttgaagaat cactcaacgt aagagcacag gccgatgaac
     1981 agtcagatat tgttggaaaa ctatataaag gaagtgtggc agacatcgta gaaaacgatg
     2041 gcacatgggc tcacatcaag tcaggaaatg tagacggata tgtcaatgta tcttactgtg
     2101 tgaccggaac tgacgcacta agctatgctt acgacacatg cggagaaatt gctacagtaa
     2161 atacagacgg acttcgtgtg agagagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnngcagtag tctgtgattc acagacagga tatatcgcag cagattatgc
     2341 gacacgttcc ctcaacacaa gggtgggtat cacaattgaa gaggaacagg cacagatagc
     2401 tgcacagaaa gaggcagaga gaaaggccgc agaagaaaag gcagccgcag aggcagccaa
     2461 agcagcaaag aaaaatactt catcaaagaa ggctgagacc acacagggcg aggcggtaag
     2521 tgcaggagca gatgacctga cactgcttgc ggcaattatc gagtgtgagg caggcggaga
     2581 gagctatgaa tgtcagcttg cagtaggagc ggcagtcatc aacagagtaa agagcagctc
     2641 atatccaaac agcatctcag gtgttattta ccagaaaggc cagtttggac cggcatcatc
     2701 aggaaagctc gcaagaaagc ttagcggacg catttcttct tcatgctaca gtgcagcaca
     2761 ggaggcaatg tccggagtgg acaacacagg aggatgtaca tccttcaatg accatggctc
     2821 aggaatatca atcggaaata tgaagttcag ataatataca gccaatacaa agaaataaca
     2881 aagaggctgc aaatcaacaa agcttgttgg tttgcagcct ctttaattat ttgtacggcg
     2941 tggttttaca gtcagagcgt caatgcttca aacctcttgc aacataccac aaaatagagt
     3001 aaaatgacat cattatagca acctacagat attaattata gtaacctgca tctataaacg
     3061 atgcaaataa aacccaggag gcaagagcaa tgcagttaaa gggacgaaac tttttaaaac
     3121 ttatggatta cactccggaa gagattactt atctgattga cctctcacag gagctgaagg
     3181 aaaagaagaa aaaaggcata agacatgatg aactcacagg caagaatata gcgctgattt
     3241 ttgaaaagac cagtaccaga actcgctgct cttttgaggt agcggcacat gaccttggaa
     3301 tgcatgtcac ataccttgac ccgagcggtt cacagatagg caaaaaggag agtatcgccg
     3361 atacagcgcg cgtgctcggc agaatgtttg acggaatcga gtaccgtgga tacggacagg
     3421 aaatcgtgga ggagctggct aaatacgcag gcgtaccggt atggaacggc ctgacaaatg
     3481 agttccatcc gacacagatg atagcagata tgcttacaat ccgtgagcac ctcggcagac
     3541 tcaagggcgt gaaatttgta tacatgggcg atgcaagata caacatgggt aattcactca
     3601 tggtcacatg tgcaaagctt ggtatggact tcgtggcatg cacaaacaag aaatatttcc
     3661 caaatgatga gcttgtaaaa tactgtaagg acgttgcaga aacaaccggc gcatcaatca
     3721 cactcacaga ggatgtggca gagggcacaa aggacgcaga tgtcatctac acagatgtgt
     3781 gggtatcaat gggcgagccg gaagaggtat gggcagagcg aattaacgac ttaaagccat
     3841 atcaggtaaa tgcaaaagca tttgaaaatg caaaggatag cgcaatcttt atgcactgtc
     3901 tgcctgcatt ccatgattta aagaccacta tcggcaagca ggtctatgag aagtttggac
     3961 tgagcgagct tgaggtgaca gacgaggtat ttgagtcaga gcgctcagtg gtatttgatg
     4021 aggcagagaa ccgtatgcac tcaatcaagg ctatcatgcg tgcaacactt gctgattagt
     4081 acggcaagca atgaccgcaa caaaagattg acggaaagct aaggaataaa gtatgaagga
     4141 agaaatactt agacttctac gcagcgctga cggctatatt tcagggcagg agctatgcaa
     4201 ccggttcgga gtatcgcgca cagctgtgtg gaaggccata aaccagctaa aggaagccgg
     4261 atatgagata gaggcacaac agaataaggg ctacaggctc atggcagcgc cggatctgat
     4321 gacagaggcg gagataaaaa gcctcatgca cactgactgg gtggcaaagg aagtgctgta
     4381 ttttgataca atagactcca ccaacatcaa ggctcaggag cttgccgaaa aaggctatcc
     4441 gagcggcacc ttagtcgtag cggacaagca ggagtcaggc aaggggcgcc gcggccgcag
     4501 ctgggtatcg ccgtccggca caggtatttt catgacactt atgataaagc ctgatattaa
     4561 tcccaacaat gcatccatgc tcacgcttgt ggcagcgctt gctgtcgcaa aggcaataac
     4621 aagcgttaca ggcgaggaag ccctgataaa gtggccaaac gacattgtga taaacggcaa
     4681 aaaggtgtgc ggcatactca cagagatgaa tgcgcagttt gactacatca accatattgt
     4741 tgtaggtatc ggtatcaacg tgcataatga gagctttccg gaggaaatca gtcagatggc
     4801 gagctcactc atgatagaag caggtggcaa aagatttcac cgtgcgcaga taatcgcaga
     4861 gaccatgtca tactttgagc agtattatga cacattttta aagacgcagg acctttcagc
     4921 cctcgtcagg gagtatgatg agctattggt caacaggaac aaatcagtaa gggtactgga
     4981 tccgaaggag ccgtttgacg gaaaagcgat gggcatcacg cctaagggcg agctgatcgt
     5041 ggacacatgg gaatcgcgta agctggtttc aagcggagaa gtatcagtga gaggaatata
     5101 cggatatgtc taaagaggta gtactttgcg tgtcaaacgc atatcagaaa aaatattatc
     5161 tgaacgagaa ttttaacgga cttccacaga ctataaggga cgagctcaaa atcatgtgcg
     5221 tcctgtatac agaggatgtg ggaggcatac tggagctggt gtttgatgag gacggaaacc
     5281 tcgatttcag aacatcctgc aaggaaaacg actttttcta tgacgagata ggaagcgtgc
     5341 taaagataaa gcagtaccgc aacgagcgaa gagagctgct cgagtcgata gagacgtatt
     5401 atcgcatagt atttttggga gaaggactcg aggaggaata acagatgatt ttagcactgg
     5461 acgtaggaaa tacaaacata acatgcggcg tgtttgatgg tgacaggata aaagcgagct
     5521 ttagaattac aactaaaatg ccacgtacat cggatgaata cggcatgctt ctgagcactc
     5581 tgcttgaaag aaatcaggta ggcatggacg acatacatga tgcaatcatc tgctccgtcg
     5641 taccgaatat catgcattca ctgcagaatg gcctaataaa atacttcaat atcaggccga
     5701 taatcgtgga ggcgggcatc aaaacaggca tccgtatcgc cacaccaaat ccgcagcaaa
     5761 ttggcgcaga ccgtatagta gacgccgtag ctgcatatga attatatgga ggacctgttt
     5821 tggtcataga ttttggcaca gcaaccacct acgatatggt cgatgaaaac ggtaccttca
     5881 tgggaggaat cacagcacca ggcatacgta tctcagccaa ggcactctgg gaggatgcgg
     5941 caaagcttcc tgagatagag attaaaaagc ctgataatat cctcggaaag gacactatca
     6001 ccagcatgca ggcgggactt gtgtatggac agataggaca gacagagtat atcatcaaca
     6061 aggtaaagga agaaacaggc atgtacgatg caaaggttgt agtgaccggc ggactcggca
     6121 gaataatatc caatgagaca gaaaacgtgg atgtatacga tccggatctg acattaaagg
     6181 gcataaacct cgtgtatcgc aagcagaacc gcaagggagt gaagtaatag tatggatatt
     6241 catcagatac gaaagcttca gataggaagt gtgacactcc caaacaacct gatactggga
     6301 cctatggcag gcgtgacaga cctgccattt cgcttattgt gcaaggagca gggagccggc
     6361 cttttatgta tggaaatggt cagcgccaag gcaattttgt acaaaaacaa aaatacagag
     6421 tcgcttcttt caatagatga aagagaaaag cctgtttcac tccagatgtt tggctcagat
     6481 ccgcagataa tggctcagat agcaaagcag atagaggaaa gaccgtttga catattggat
     6541 atcaacatgg gctgtccggt gcccaaaatc gtaaacaacg gtgaaggctc agcactcatg
     6601 aaaaatccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnncat gtggctgaag aaagcggcgc aagcgcagtc gcagtgcacg
     6781 gcaggaccag agagcagtac tactccggta aagccgactg ggacatcata aggcaggtaa
     6841 aagaggctgt ctcaataccg gtcataggaa acggagacct gctttgcgca gaagatgtca
     6901 taaaaatgca ggagcagaca ggctgcgacg gctttatgat agccagggga gcgcagggca
     6961 atccatggat tttctcacag atactgcatt actttgagac cggcgagcat caggaaaagc
     7021 catcctttga ggaagtacgc cggatgatac tgcgccatgc acgcatgatg atagagttca
     7081 agggcgagta cacggggata cacgagatga gaaagcacac agcgtggtat acggcaggat
     7141 atccgcattc atcaaggctg cgcgcggcag tgaatacggt ggagagctta gaacagcttg
     7201 ag
//
LOCUS       DPUF01000169            5280 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4621, whole
            genome shotgun sequence.
ACCESSION   DPUF01000169 DPUF01000000
VERSION     DPUF01000169.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4621"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="DHV81_06070"
     CDS             complement(<1..647)
                     /locus_tag="DHV81_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCI94032.1"
                     /translation="MYKIIIDSCGELTDSLKKDGHFCNVALELDVDGYRIRDDESFDQ
                     HDFLRRVKESVKGPKSSCPSPEEYMQAFEGEADHVYVVTLSGKLSGSYNSAVLAVNLY
                     NEEHEDCGKQIYVFNSRSASIGETLIGMKVQEFEESGLSFDEVVKQTEEYISSMNTFF
                     VLETLDTLRKAGRLSNLKAFVASTLNIKPVMGSTDEGSIQQLGQARGIKRALAKMV"
     gene            complement(811..2097)
                     /locus_tag="DHV81_06075"
     CDS             complement(811..2097)
                     /locus_tag="DHV81_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HCI94033.1"
                     /translation="MKQEKLFDLLKAAVSPCECVKAAKQELLENGFEEIDYIGDWKLV
                     RGGRYVLNHHDTTMFAFTVGSGYNKKDMVRIAAAHTDYPYLRIKPNPDFMTNSYAQVN
                     VEVYGGPILNTWFDRPLGVAGRVAIKSEDVFNPRMVLYRSKKPVMIIPNLAIHMNRDV
                     NKGVGINNQVDLMPVLDSITEDEMTTDYFLSFLARELSVEKSDIIDFELNTFCMEEPC
                     FVGVNDTMISSPRIDNQSSCRALLDAIEDGNRADGINIIALFDHEEIGSNSKQGAASI
                     MLHDMLRRILRNMDLSENEIDESIYDAMLLSVDVAHALHPNKKEKMDITNKPVMGKGF
                     CIKQACSQSYATDAQAIAILCQLCDEKGIPYQRFVNRSDSRGGSTLGSIAGTLLPVKT
                     VDIGIPILAMHSACELMGVRDMKALSDCVTAFFGYH"
     gene            2313..2816
                     /locus_tag="DHV81_06080"
     CDS             2313..2816
                     /locus_tag="DHV81_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94034.1"
                     /translation="MISIELTDTKDFMNKLLRTEIFDNFLLQEVVITKAASYVIDGHL
                     QKGFYSSTELEENGIAGYSILPFRMLRTNCFDLIKGRQTPSSFKFVFLLSPENMEHTL
                     SSLHSAFTVSDISGFFINIRYQSQLLTLTTGISYNIFSADKTLDSEWDKLVMKFLANN
                     DINFKEL"
     gene            2902..3105
                     /locus_tag="DHV81_06085"
     CDS             2902..3105
                     /locus_tag="DHV81_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCI94035.1"
                     /translation="MSKGTVKWFNNQKGYGFITAEDGKDVFVHFSGLNMEGFKTLEEG
                     AAVEFDITDGAKGPQAVNVEVVK"
     gene            complement(3279..4769)
                     /locus_tag="DHV81_06090"
     CDS             complement(3279..4769)
                     /locus_tag="DHV81_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase"
                     /protein_id="HCI94036.1"
                     /translation="MLKRIGKMDKNKKMIIGILTAAIVLVVAFIVYITCFDSHIEFSS
                     KFKNGITVEYGKKFEVPKIKAYVRGRLINRKGKEIKCTIDSNVDATKTGSYEIKVTAQ
                     YGKKTATQTIKVEVRDKKAPEITLNGDAEMTVEAGSKFSDPGYTATDNYDGDLTGKVS
                     VTGAVDTSKPGDYEIKYSVADSSKNESEVKRTVHVTDTTAPQIKLSGDDFMSVKKGDK
                     YSDPGYTATDNCDGDITDSVKVSGDKVDKDKAGKYTVTYEVSDSSGNKATATRVVSVY
                     DPAATADTVNPGNKIIYLTFDDGPGKYTQGLLDVLDKYNVKATFFVTNTHPDYQNMIA
                     EEAKRGHTVAIHSASHKYNQIYTSEQAFFDDLEQMNSIIKAQTGNDASIIRFPGGSSN
                     TVSKDYCPGIMTQLVNDVTARGLLYCDWNVSSGDANSKPISTEQVVQNVISGVQSHNV
                     SVVLQHDIKDFSVNAVEQIIQWGQANGYTFLPLTTSSPMSHHRINN"
     gene            5201..5280
                     /locus_tag="DHV81_06095"
                     /pseudo
     CDS             5201..5280
                     /locus_tag="DHV81_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start and stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1408 a         1229 c          959 g         1684 t
ORIGIN      
        1 accatcttgg caagtgccct cttaatgcct ctggcctggc caagctgctg gattgagccc
       61 tcatcggttg agcccatgac aggctttata tttaaagtgc tggccacaaa agccttcagg
      121 ttgcttaatc gtcctgcctt tcttaatgta tcaagtgtct caagcacaaa gaaggtattc
      181 atggaagaaa tatactcctc ggtctgcttt acaacctcat caaatgaaag tccactctct
      241 tcaaactcct gtaccttcat gccgattagt gtctcgccta tagatgctga gcgggaattg
      301 aatacataga tttgcttgcc acagtcctca tgctcctcgt tgtacagatt gactgctaaa
      361 acagcgctgt tgtatgagcc gctgagcttg cccgaaagtg tgacaacata tacatggtca
      421 gcctctccct caaaggcctg catatattcc tcaggagacg ggcatgacga ctttggtccc
      481 tttacactct cctttactct tcttaagaaa tcatgctggt caaagctctc atcatcgcgg
      541 attctgtatc cgtcaacatc aagctcgagt gccacattgc aaaaatgtcc atccttctta
      601 aggctatctg tgagctcacc acagctatct attataatct tatacatatc tattcctcct
      661 gcactatatg ataataaaag tttaaattac ttttttaaaa gttatttcat tccgtaacaa
      721 gtagttttca ttactacgtc atattaacac gaaagctaaa cttatgcaag aggtttctgt
      781 atttattttc ccataatggt tatctgccct ttaatggtac ccgaaaaagg ccgtcacaca
      841 gtcgcttaag gctttcatgt cgcgtactcc cataagctca caggctgagt gcattgcaag
      901 aataggtata cctatatcca cagtctttac cggcaaaagc gtacctgcaa tcgagcccag
      961 tgtgctgccg ccacgactgt ccgaacggtt cacaaaacgc tggtaaggta tgcccttttc
     1021 atcacacagc tgacagagaa tggcgatagc ctgtgcatcc gtagcatacg actgcgaaca
     1081 cgcctgcttg atacaaaagc ctttacccat gacaggctta tttgtgatat ccatcttttc
     1141 ctttttatta ggatgaagcg catgagccac atcaacagac aaaagcatgg catcatatat
     1201 gctctcatct atttcatttt ctgaaaggtc catatttctt aaaatacgtc tgagcatatc
     1261 gtgaagcatt atagaggcag cgccctgctt actgttactt ccaatctcct catggtcaaa
     1321 aagcgctatg atgtttattc catcagctct gtttccatcc tctatcgcat caagaagagc
     1381 cctgcaggat gactggttgt cgattctcgg tgatgaaatc atggtgtcat tcacaccgac
     1441 aaagcaaggc tcctccatac aaaatgtatt gagctcaaag tctatgatat cgctcttttc
     1501 cacagacagc tctctagcca gaaaactaag gaaataatcc gtggtcattt catcctcagt
     1561 tattgaatca agcaccggca tcagatccac ctgattattt atcccgacgc ccttattcac
     1621 atccctgttc atatgaatag caagatttgg aataatcatg acaggctttt tggacctata
     1681 tagcaccata cgcggattga acacatcctc actctttata gccacgcgcc ctgccactcc
     1741 gagaggtctg tcaaaccatg tgtttaaaat cggtcctcca tacacctcta cattaacctg
     1801 tgcatatgag tttgtcataa aatccggatt tggctttatg cgaagatatg gatagtctgt
     1861 gtgagctgcc gctattcgca ccatatcctt tttgttgtag ccgcttccta cagtaaacgc
     1921 aaacatggtc gtatcatggt gatttaagac atacctgcca ccgcgcacaa gcttccagtc
     1981 gcctatataa tctatctcct caaatccgtt ttccaaaagc tcctgctttg cagccttcac
     2041 acactcgcat ggtgaaacag ccgcttttag taaatcaaat aatttttcct gtttcatgga
     2101 ttgcctcctg ttttcattgc ataacgcatt tattctacca cggactttac aattattcca
     2161 gttgaacagg ctttattttt aaggctattt ttatatctat ttttatggca taatccgcga
     2221 atctgatgta tgatataata atcgataact attcaaaacc acttagaaca atcattcgct
     2281 gtcacataca atgtggaaaa tgaggagaaa ttatgatatc aattgaactg acagacacaa
     2341 aggactttat gaacaagctg cttcgcacgg aaatctttga caacttcctg ctccaggagg
     2401 tcgtcatcac aaaggcagcc tcatacgtca tagatggtca cctgcaaaag ggcttttaca
     2461 gctccacaga gcttgaggaa aatggtatag ctggctatag catactcccc tttaggatgc
     2521 ttcgcacaaa ctgctttgac ctgataaagg gcagacagac tccatcgtca ttcaagtttg
     2581 tctttctgct ttcacctgaa aacatggagc acaccctaag cagtctgcat tctgctttca
     2641 ccgtgtccga tatcagtggt tttttcataa atatcaggta tcagagccag cttctgactc
     2701 tgacaaccgg aatatcctac aatatttttt cagcggataa gaccttagac agtgaatggg
     2761 ataagcttgt aatgaaattt ttagcaaata atgacataaa ctttaaagaa ctttgacttt
     2821 attgctttac atattaaacc tgttatgcta ttctaacgtt agttgcgtaa gcagcattat
     2881 tattatttgg aggtactttc gatgagcaaa ggtacagtaa aatggttcaa taaccaaaaa
     2941 ggttacggat tcatcacagc cgaggacgga aaggatgtat tcgtacattt ctcaggactt
     3001 aacatggagg gcttcaaaac tctcgaggaa ggtgcagcag tagagttcga tatcactgat
     3061 ggcgctaaag gtccacaggc agttaacgtt gaggtagtta aataattttg accactcagg
     3121 caatctagtt tctatgtacc tttttcttta ttatatattt aggtaaaatt atagttacaa
     3181 aaatgttaat tggggaataa gatataagaa tcatgattaa aaaagaagag ttcattttga
     3241 actcttcttt tttgtgcttt tttattgttt tggtgctttt agttgtttat gcgatgatga
     3301 gacatcggtg agcttgtggt gagtggtaaa aacgtgtaac cgttcgcctg tccccactga
     3361 atgatctgct cgaccgcatt gacactgaaa tcctttatgt cgtgctgcaa tacaactgat
     3421 acgttgtggc tctgcactcc tgaaatgaca ttctgcacta cctgttccgt gctgattggc
     3481 ttactgtttg catctccgct cgatacattc cagtcacagt ataaaaggcc tcttgctgtc
     3541 acatcattta cgagctgtgt cataatgccc ggacagtaat ccttgctcac agtatttgat
     3601 gagcctcccg ggaatctgat gattgacgca tcatttcctg tctgtgcctt tatgatactg
     3661 ttcatctgct caaggtcatc aaaaaatgcc tgctcacttg tatatatctg gttgtatttg
     3721 tggctggcag agtggatagc cactgtatgt cctctctttg cctcctctgc tatcatgttc
     3781 tgatagtcag gatgtgtatt tgtaacaaag aaggtggcct ttacattgta tttatcaagc
     3841 acatctaata gcccctgtgt atactttccg ggaccatcgt caaaggtaag ataaataatc
     3901 ttgttgcccg gatttactgt gtccgccgtg gcagccggat catacacact gaccactctt
     3961 gtagcagtag ccttatttcc tgatgaatct gagacctcat aggtcacagt atactttccc
     4021 gccttatcct tatctacctt atctcctgat accttgacac tgtctgtgat gtctccgtca
     4081 caattgtctg ttgccgtgta tcccggatca ctgtacttat cacccttttt aactgacata
     4141 aaatcatcac ccgaaagctt tatctgcggt gcagtggtat ctgtgacatg cacagtcctt
     4201 ttgacctcgc tctcattctt tgacgagtca gcgacactgt actttatctc atagtctccc
     4261 ggctttgaag tatcgacagc tcctgtgaca gataccttgc ctgtcagatc cccatcatag
     4321 ttgtctgttg ccgtatatcc cgggtcactg aacttactgc ctgcctctac agtcatctct
     4381 gcatcgccat ttaatgtaat ctccggagcc ttcttatccc tcacctcaac cttgatggtc
     4441 tgtgtcgcag tttttttacc gtattgagca gttaccttta tctcgtatga acctgtcttt
     4501 gtcgcatcga cattactgtc tatagtgcat tttatctctt ttccctttcg gtttataagc
     4561 ctgcctctga catacgcctt gatttttggt acctcaaact ttttgccata ctcaacagta
     4621 ataccgtttt tgaacttgct gctgaactca atatgtgaat caaagcatgt gatatacact
     4681 ataaacgcca ccaccagaac tattgcagcc gtaagtatgc cgataatcat ctttttattt
     4741 ttgtccattt ttccaatcct ctttagcatt ttataattat tctctctata ttattactca
     4801 aacctgataa tattatttca agcagtatct attatagcgt taaacaatct tttttgctac
     4861 tgcaaattcc ttgagaattt cttaatgttg gcttcatata tttcatttat gttacaattg
     4921 tttctttatc atgaatacct ccggtgcagt cttaataagt aattaattta tttaatatat
     4981 ttgttactat tcagttctca atatgactga ccacatatct ttttgcacat attcgcaatc
     5041 acaaaaatcc cccgaacaag tcgggggatt taaaaagatt atgctgtaac aatcatttta
     5101 ttactgctcc ttacggtgca tctcaaatgt ctccatatgg tagttggcga tgttgtcacg
     5161 aatctttctg tcatctgctc tgcttaaaag atcatcacgg aacttcatcg agggaatggc
     5221 atgtgagggc ggctgcgtag gcggaccaag ctcatacaat gacatggtga ctaccaagaa
//
LOCUS       DPUF01000170            4734 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_30251, whole
            genome shotgun sequence.
ACCESSION   DPUF01000170 DPUF01000000
VERSION     DPUF01000170.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_30251"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="DHV81_06100"
     CDS             complement(<1..335)
                     /locus_tag="DHV81_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCI94037.1"
                     /translation="MKEKSRSTFSGNIGFVLAAAGSAVGLGNIWRFPYLAAKDGGGLF
                     LLVYLILVLTFGFALLTTEIAIGRKTGQGPLTAYGLINPKGKFIGVLACIVPAIILPY
                     YCTIGGWVL"
     gene            complement(833..1699)
                     /gene="metF"
                     /locus_tag="DHV81_06105"
     CDS             complement(833..1699)
                     /gene="metF"
                     /locus_tag="DHV81_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HCI94038.1"
                     /translation="MKITQIHTNKPQSLSFEIFPPKKEEDFKNIDEMLEILCDCHPEY
                     ISVTFGAGGSSNNNKTIEIAKKIKNQYNVEPVVHLTCLCYNKAEIDEFTRQLSYEGIE
                     NILALRGDRNPNVPAKEDFLHASDLIKYIKETTGDQFCIAGACYPDIHPEAADKVDDI
                     KNLKKKVDAGAELLLSQLFFDNDTFFNFAEDCRLAGINVPVIPGIMPCINAAQIQRMV
                     TMCGAKFPEKFQKIVHRYGDNKAALFDAGMSYCESQIIELLANDVEGIHLYTMNNVRV
                     AKRLTEGIKNLI"
     gene            1897..3258
                     /locus_tag="DHV81_06110"
     CDS             1897..3258
                     /locus_tag="DHV81_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI94039.1"
                     /translation="MKKIDFDNDSIKKNILQAALPMLAAQILSLLYNIVDRIYIARIP
                     DMGTTALGAVGLCFPIIMLTIAFSNLFGSGGAPLFSIKRGMSDDRTANTIMNTSFTMV
                     CTFAVVFTALCMIFAKPLLIVFGASENALKYALPYLLIYLIGTLPSMISTGMNPFINA
                     QGYSTTGMLSVAIGAVANLVLDPLFIFGFNFGIKGAAIATVISQILSALYVLHFLRKK
                     AELKVRLMTLSEFSENTRYAKDIISLGTSGCVMQLTNSLVSICCNNVLSVTGGDLYIS
                     VMTIVSSIRQMVETPIYAIVEGSSPVLSYNYGAKRPARVRKSIFTMGLLVLLYTAVMW
                     SVIILAPHALIAIFSSDSTLMDDAVKALNIYFAAFIFMDLQYIGQTTFKSLNKKKRAI
                     FFSLLRKVFIVVPLTYILPYSFGMGTDGVFMAEPVSNVIGGSICFVTMLGTVLPELRR
                     MEQ"
     gene            complement(3259..4575)
                     /locus_tag="DHV81_06115"
     CDS             complement(3259..4575)
                     /locus_tag="DHV81_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCI94040.1"
                     /translation="MRKFFGKFVSVTLAAAVIAMFALSSDDKWCSRVDKAFDESALGS
                     FLNESKAGYGRYATGLPGQAASVLADSGENGENGENGGTGENSGNGGTEQGIGQTADT
                     ASTHRDYEETDKISAGISVEGVYACGRLTGIYEQTEGVLVVNTTEVTDEDGKKVNPAD
                     KKVQCGDYILSVNGRTVADKEELSEAVNDIMKQHDESHEDKSTVNIKFLRGGEKMSAD
                     ITPVRMDDGKYYMGIWVKDDLAGIGTITYYTKDGRFGALGHGIGDGTQSGNLLYANSG
                     DLYSMKLTKIKKGKAGAPGEIGGVVYFGKKSHIGTLDCNSNLGIYGQLDSDELSEYAA
                     EDTYYPVAGKDEIHTGSAQMISEISGKLEKYNLEITNIDKKATDTNKGMELKVTDDRL
                     IELSGGIVQGTSGSPIIQDGKIIGAVTHVFVDDPTGGYGICIDEML"
BASE COUNT         1179 a         1155 c          906 g         1494 t
ORIGIN      
        1 tttaaaaccc atccacctat agtacagtag taaggcagaa taatagccgg tactatacac
       61 gcaagcacac cgataaactt gccctttgga ttgataagac catacgcggt gagcggtccc
      121 tgtccggtct tacgtcctat tgcaatctca gtggtaagca atgcaaagcc aaaggtcaga
      181 acaagtatca gatacaccag cagaaaaagt cctcctccgt cctttgctgc cagatacgga
      241 aacctccata tatttccaag tcctaccgca ctgccggctg ccgcaagaac gaacccaatg
      301 ttgccggaaa aagtgcttct tgatttttct ttcattttct actgtctcct taatatatta
      361 tataatttaa tttcgattta atatggacat tgtttccatt tacaaatcta atatacctat
      421 tataatcatc tagagtactt tttgcaacgg ttgatattat ttattcttgt gtggtaggac
      481 atggcaggtg tcgtgagtgc tacgctacac tgtcaagata ctgtaagaat tctgtcacag
      541 cgtaaggaac gtaagccccg ggtaccgtgg gcgcacacgt actcccgccg gagcgcatag
      601 cggagcggct tagatactgt agaaattatg cccaagagac acagaaacgc ccctgcatgg
      661 acgcaggggc gaggagaacc gcgccacgga cggcgcgttt cgacggtttc gtcaagcata
      721 gaaacccatc aagcataatt tcgtacagta tcttagccac gcagcgtagc gacggcagga
      781 gtacgtgtgc gcccgtggta cccggggctt gcgttccgta ctatttattg tcctaaataa
      841 ggttcttgat tccctctgtt aaacgctttg caactctcac attgttcata gtgtaaagat
      901 ggattccctc cacatcatta gctaaaagct caattatctg actctcgcag taagacattc
      961 cggcatcaaa aagtgcagcc ttgttgtctc cgtatctgtg cacaatcttc tggaacttct
     1021 ccgggaactt tgctccgcac attgtgacca ttctctgaat ctgagccgca ttgatacaag
     1081 gcatgatgcc cgggataacc ggcacattga tacctgcaag cctgcagtcc tcagcaaaat
     1141 tgaagaaggt atcattgtca aagaaaagct gtgataaaag aagctctgct cctgcatcaa
     1201 cctttttctt aagattctta atatcatcta ccttatcagc tgcctccgga tggatgtccg
     1261 ggtagcacgc acctgcgata cagaactgat cacctgtggt ttcctttatg tacttaataa
     1321 ggtcgcttgc atggagaaaa tcctcctttg caggcacgtt cggatttcta tctcctctta
     1381 aagccaggat attctcaatg ccctcatatg aaagctgtct ggtgaactca tctatctctg
     1441 ccttgttata gcacagacat gtaaggtgta cgaccggctc cacattgtac tggttcttga
     1501 ttttcttcgc aatctcgata gtcttgttat tgtttgagct gccgcctgcg ccaaatgtca
     1561 cgctgatgta ctcaggatga caatcacaga gtatctcaag catctcgtct atatttttga
     1621 agtcttcctc cttctttggt ggaaatatct caaaggagag cgactgtggt ttgtttgtat
     1681 gtatctgtgt aatcttcatg ttatcctccg gtatatatgg ttattcaata atttgtaatt
     1741 tttatattcc atatattact gtagtttttt gtcattgtaa aatatatttt tcttttagtt
     1801 tgtcataaat aaaatctata tcaaaacttg ctgagcattt gattttttta tatgaggtga
     1861 tataatgggc gaagcaaatc aaaaagaggt ttcaaaatga aaaaaataga ttttgataat
     1921 gattcgataa agaaaaatat attgcaggct gctctgccca tgctggcagc ccagattcta
     1981 agcctgttgt acaatatagt agaccgcata tacattgccc gcataccgga tatgggaacc
     2041 actgcactcg gtgctgtggg gctttgcttt cctattataa tgctgacaat cgctttctcc
     2101 aatctctttg gaagcggtgg tgctccgctt ttttcaataa aaagaggcat gagtgatgac
     2161 agaaccgcca atactataat gaatacgagc tttacaatgg tctgcacatt cgcagtcgtt
     2221 tttacagctt tgtgcatgat ttttgcaaag cctcttttaa tcgtgtttgg cgcatctgaa
     2281 aatgctctca aatacgctct gccatacctg ctcatttatt tgataggaac actcccatcc
     2341 atgatttcaa ccggcatgaa tccctttatc aacgctcagg gctactccac taccggcatg
     2401 ctgtctgttg caatcggagc cgttgccaat ctggttttag acccgctttt tatatttgga
     2461 tttaatttcg gtatcaaagg agcagctatt gctacggtca tctcacaaat actgtctgct
     2521 ctctatgtgc ttcactttct taggaaaaag gctgagctta aggtgcgcct tatgacactc
     2581 tctgaattca gtgaaaacac gcgctatgca aaggacatca taagccttgg aacctcaggc
     2641 tgtgtcatgc agctcacaaa cagcctggta tctatatgct gcaacaatgt gctttcagtg
     2701 accggtggcg atttgtacat atcggtaatg accatcgtat caagcatcag gcagatggtc
     2761 gagactccaa tttatgcaat tgtggagggt tcatctcctg ttttaagcta caactatggc
     2821 gccaaaaggc cggcccgggt gagaaaatct attttcacta tgggactgct cgtgcttcta
     2881 tatacagctg tcatgtggag tgtgattata cttgcccctc atgccctgat tgcaattttc
     2941 agttccgatt ccacccttat ggatgatgcg gtgaaggctt tgaatatcta ctttgcggca
     3001 ttcatattta tggatctgca gtacatcgga cagaccacct ttaaatcact gaacaaaaag
     3061 aagcgggcta tatttttctc cctgcttcgc aaggtgttca tcgttgtacc gctgacatat
     3121 attctgcctt actcctttgg tatgggaacc gacggtgtgt ttatggcaga gcctgtatca
     3181 aacgtgattg gcggaagcat ctgctttgta accatgcttg gcactgtgct acctgagctt
     3241 cgcagaatgg agcagtaatt acagcatctc atcaatgcag atgccgtagc ctccggtcgg
     3301 atcatcgaca aacacatggg tgactgctcc tattatttta ccatcctgta tgatagggct
     3361 tccggatgtg ccctgcacta tgccaccgga gagctctatc agcctgtcgt ctgtcacctt
     3421 aagctccatt cccttgtttg tatctgtcgc ttttttatct atatttgtta tttccagatt
     3481 atatttttca agttttcctg atatttcaga tatcatctgt gcactgcctg tgtgtatctc
     3541 atccttgccg gcaaccggat aatacgtatc ctctgcggca tattccgaca attcatcgct
     3601 gtcaagctgt ccgtaaattc ccagattact attgcagtca agtgtgccta tatggctttt
     3661 tttgccaaaa tataccactc cgcctatctc gcccggcgcg ccggcttttc cctttttgat
     3721 tttggtaagc ttcatgctgt acaaatcacc actgtttgca tacaagagat tgccgctttg
     3781 cgttccgtct cctattccat gccccagcgc tccaaaacgg ccgtcctttg tataataagt
     3841 gatagtaccg attcctgcca gatcatcctt cacccatatt cccatatagt atttgccatc
     3901 atccatcctc acaggcgtga tatccgctga cattttctca ccgcctcgaa gaaatttaat
     3961 gtttacggta cttttatctt catgactttc atcatgctgc ttcataatat cattcacagc
     4021 ttcagaaagt tcctccttgt ctgcaacagt gcgtccattg acagatagga tataatcacc
     4081 gcactgcacc tttttatctg ccggattgac tttttttcca tcctcgtctg tcacctccgt
     4141 cgtattcact acaagcactc cctctgtctg ctcatatata ccggtcagcc tgccacaggc
     4201 atatacgccc tcaacactta ttccagcact tattttatct gtctcttcat aatctctgtg
     4261 agttgatgct gtatctgctg tctgtcctat gccttgttcg gttccaccgt ttccactgtt
     4321 ttcaccggtt ccgccgtttt caccattttc accattctcg cccgaatcag ccaatacact
     4381 ggccgcctgt cccggaagcc ctgtcgcata tcttccatat ccggcctttg attcatttaa
     4441 aaaacttcct aatgcacttt catcaaatgc cttatctacc ctgctgcacc acttatcatc
     4501 actgcttagt gcaaacatag ctatcactgc tgcagccagc gtgacactca caaattttcc
     4561 aaaaaacttc cgcatactat tacctccatt tggattagta tgctcctgta atttttcttt
     4621 tattctttta atatcttatt atctcattct aactattcca aattattttg agcaaaaacc
     4681 agtatagttt gacagtttag gtgttttcct atataatttc gatatattta tatg
//
LOCUS       DPUF01000171            3370 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_14837, whole
            genome shotgun sequence.
ACCESSION   DPUF01000171 DPUF01000000
VERSION     DPUF01000171.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_14837"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            188..1114
                     /locus_tag="DHV81_06120"
     CDS             188..1114
                     /locus_tag="DHV81_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCI94041.1"
                     /translation="MKKKISIMLCIVLFALTGCTNSSSTIRFGAADIGGMYYSFANTF
                     TELANEQGYDFTCKVRTTAGSNANIRLLSDGYIEIGIAQADLIADAYKTNEDLRAIAG
                     LYTETCQLVVRADSDIQTLDDLSGHTVSIGAEESGTERNATQILEFAGMPSSLVATKN
                     LDYIEATKELKAGDIDAFFCTAGLTTTVIDELSKECDIRLIPLTDTVISKMLESSSAY
                     TSEVIPAGTYTGQTADISTIGVKSVLITKSDVSDDTIEQLTSLLFEKENELSYSNSLK
                     LELTYDFATGDIPIPFHNGAKRYYEDCGYSTN"
     gene            1206..2393
                     /gene="gltS"
                     /locus_tag="DHV81_06125"
     CDS             1206..2393
                     /gene="gltS"
                     /locus_tag="DHV81_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCI94042.1"
                     /translation="MKIQLDMYQTLAVAVLVLLLGNYLKKKIYFLQKFCIPAPVIGGL
                     IFAIMTCICYVTGITEFSFDDTLREVCMVFFFTSVGFQANLKVLKSGGKSLIVFLGLV
                     ITLIILQNLTAVGLAKLLNLNPLIGMCTGSIPMVGGHGTAGAFGPVLEDLNIKGATTI
                     CTAAATFGLIFGSLIGGPLGKRLIEKHSLLNTAANDDDSLLVEDEKKHERHTNMYADA
                     VFQLILAIGVGTIFTMLLTKTGLTFPIYIGAMLAAALMRNICEYTGIATIHMGEINDL
                     GGISLSLFLGMAMITLRLWELASLALPLVILLAAQVLLIIIFTYVIEFNIMGRDYDAA
                     ILVSGTCGFGTGATPNAMANMQAVCDQYVPSIKAYLLIPLVGSLFADFLNSLVITFFI
                     NLL"
     gene            2411..2617
                     /locus_tag="DHV81_06130"
     CDS             2411..2617
                     /locus_tag="DHV81_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94043.1"
                     /translation="MFKKLRKIFKNREEKEKHNYDLTFDNVAIPEVHVKSTESHDDTD
                     KENAPISYDNVAIPEVHIRNRKNK"
     gene            complement(2646..3314)
                     /locus_tag="DHV81_06135"
     CDS             complement(2646..3314)
                     /locus_tag="DHV81_06135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94044.1"
                     /translation="MPLRGLMYFSKMYDRYIVEHSYNIYGSTLVKLPTPRYTVLYNGT
                     SKQPAFMKLKLSDAFIHEDTSGDFEWTANMVNINHGMNDELLNNCRPLQEYMLLIEEI
                     RKNRSNGMEVEQAVDKAVTYCINNNILSEFLTKHRAEVIDVCITEYDEQAFVNGIREE
                     GRQEGRKEGRKEGRQEGRALTLFSLVNSGNLKPDIAAKELGISIHEFEIAMKEAGMNW
                     PVSK"
BASE COUNT         1053 a          772 c          641 g          904 t
ORIGIN      
        1 atgtcaaaga tttccataaa taatttcctt cctttaaaat attttctaca actaatttat
       61 tatattattt ttacaatcac aagtctatgg aatttaagaa aaatttaaat taaatctttt
      121 catattagaa aattttttgt aaaatactca tagcaattat gaaaaaatga aaggataaag
      181 acaaaacatg aagaaaaaaa tcagtatcat gctttgcata gtactcttcg cactgacagg
      241 ctgcaccaac tccagcagca caatacgctt cggagcagcc gatattggcg gaatgtacta
      301 ttcatttgca aatacattta cggagcttgc caatgaacag ggctatgact ttacctgcaa
      361 ggtccgtacc acagcaggct ctaatgccaa tataaggctt ttatccgatg gctatataga
      421 gataggcatc gcacaggcag acctgatagc cgacgcatac aagacaaatg aagatttaag
      481 ggctatagca gggctttaca ccgagacctg tcagcttgtg gtccgcgctg attcagacat
      541 acagactctt gatgacctgt caggacacac tgtaagcatc ggagcagagg aatccggtac
      601 cgagcgaaac gccacacaga tccttgagtt cgccggtatg ccatcatccc ttgtagccac
      661 caaaaacctg gactatatag aggccacaaa agagctaaaa gccggtgata tagatgcctt
      721 cttctgcact gccggactca ccaccactgt catagatgag ctctcaaagg aatgcgacat
      781 aaggcttatc ccgctgactg atactgtgat aagcaagatg cttgaaagca gctctgccta
      841 cacaagcgag gtcatacctg ccggcacata cacaggccag accgcagata taagcactat
      901 aggtgtaaaa tctgtgctta tcacaaagtc tgatgtatcc gacgatacaa ttgagcagct
      961 tacatcgctc ctgtttgaaa aagaaaatga gctctcctac tccaactctc ttaagcttga
     1021 gctgacatat gactttgcaa caggcgatat acctattcca ttccacaatg gagcgaaaag
     1081 gtattatgaa gactgtggat atagcacaaa ttaattcgcg aattaatttt tagttcaata
     1141 tataatattc aaaatataag aagaaaataa caagaatata ataaattaaa attgaaaggt
     1201 aacacatgaa aatacaactt gacatgtacc agacactggc cgttgcagtg cttgtactac
     1261 tacttggaaa ctatttaaaa aagaaaatat attttcttca gaaattctgt attccggcac
     1321 ctgtcatagg cggactgatt ttcgccatca tgacatgcat atgctatgtc acaggaatca
     1381 cagaattttc ctttgatgac acactgcgcg aggtctgcat ggtattcttc tttacatctg
     1441 taggcttcca ggcaaatctc aaggtattaa aaagcggagg caaatcgctc atcgtatttt
     1501 tgggacttgt tatcacactt atcatattgc aaaacctaac cgccgtgggg cttgcaaagc
     1561 tcttaaactt aaatccgcta atcggcatgt gcaccggctc tatccctatg gtaggcggac
     1621 acggcaccgc aggagccttt ggcccggtgc ttgaggactt gaacataaag ggcgccacaa
     1681 ccatctgtac agcagcagct acctttggtc tcatctttgg aagcctgata ggcggacctc
     1741 ttggaaaaag actgattgaa aagcatagcc tgcttaatac agcagcaaac gatgacgaca
     1801 gcctgctggt ggaggatgag aaaaagcatg agcgccacac aaatatgtat gctgatgccg
     1861 tattccagct catcctggca ataggtgtcg gaaccatctt tacaatgctg ctcacaaaga
     1921 cagggctcac cttcccgata tatataggag cgatgcttgc agccgcactc atgcgaaaca
     1981 tatgtgagta cacaggaatc gccaccatcc atatgggaga gataaatgac ctgggaggta
     2041 taagcctctc tctattccta ggcatggcaa tgatcacact aagactatgg gagcttgcat
     2101 cactcgctct cccgctagtc atactgcttg cagcacaggt gcttctcata atcatattca
     2161 cgtatgttat cgagttcaac atcatggggc gcgactacga tgcagccata cttgtatcgg
     2221 gcacctgcgg cttcggaacc ggagccaccc caaatgccat ggcaaatatg caggctgtct
     2281 gcgaccaata tgtgccatcc attaaggcat atctgctcat accgctcgta ggaagcctgt
     2341 ttgccgactt tttgaacagc cttgtaatca cattctttat aaacctactg taaaacaacc
     2401 ggagaaaaat atgtttaaaa aattgagaaa aatatttaaa aacagagaag aaaaagaaaa
     2461 acataattac gatttaactt ttgacaacgt ggcaatccca gaggtgcacg taaaaagcac
     2521 cgaaagccac gatgacactg ataaagaaaa tgcacctatc agctatgaca atgttgccat
     2581 acctgaggtg catatcagaa acagaaaaaa taaataaaac tgataaacta aaccgtttat
     2641 ttgaactatt ttgatacagg ccagttcatt ccagcctctt tcatagcaat ctcaaattcg
     2701 tgaatgctga ttccaagctc cttagctgca atatcaggct ttaggttacc actattaacc
     2761 aatgaaaaca gtgtcaaggc tctgccctct tgcctacctt cttttctacc ttcttttcta
     2821 ccttcttgcc taccttcttc tctaattccg ttaacaaatg cctgttcatc atactctgtt
     2881 atacacacat ctatcacctc tgctctgtgc ttagtgagaa actcacttaa tatgttattg
     2941 tttatacaat aagttacagc tttatcaact gcctgctcca cttccatgcc attagaccgg
     3001 ttcttacgta tctcctcaat aagcagcata tattcctgca atggtctgca attgtttaaa
     3061 agctcatcat tcataccatg gttaatgttt accatgttgg cagtccactc aaaatctcca
     3121 cttgtatcct catggataaa cgcatctgac agcttaagct tcataaatgc cggctgcttc
     3181 gatgtaccat tgtacaacac cgtatatctc ggtgttggaa gctttaccag cgtcgaacca
     3241 tagatattgt aggaatgctc gacaatatag cggtcataca ttttgctgaa gtacatcaag
     3301 ccacgaagcg gcatgtttgg attgtaggag ctttgctgct cccatagatg taaataacta
     3361 tccagaagaa
//
LOCUS       DPUF01000172           10189 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_15095, whole
            genome shotgun sequence.
ACCESSION   DPUF01000172 DPUF01000000
VERSION     DPUF01000172.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_15095"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="DHV81_06140"
     CDS             <1..1476
                     /locus_tag="DHV81_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HCI94045.1"
                     /translation="MPIFEFDEMESARVVDGVQLYNYWGYNTVSFFAPNTSYAFNEEH
                     NHEGDELKSLIKALKENGIEVILDVVFNHTAEGNEMGPCFSFKGIDNNVYYMLTPDAH
                     YYNFSGCGNVMNCNHPVVRSFIIDCLRHWAIEYRVDGFRFDLASILGRDQNGAPMANP
                     PILESLAFDPVLGKMKLIAEAWDAGGLYQVGSFPSWNRWAEWNGRYRDDMRSFLKGDD
                     GMAGNAITRITGSRDLYSPESRGHKASVNFMTCHDGFTLYDLYSYNEKHNEKNGWNNT
                     DGDNNGHSWNCGAEGETDDPNVNGLRRRLIKNAFAALLCSRGPAMFFAGDEFCNTQFG
                     NNNAYCQDNIISWLDWSRLEEFKEIHDFVRHMIQFRKEHPILRKMTKPSSCQFPEISV
                     HNGTPFNASTDYKTKLIGIMYAGRNEEDKEDDIVFYCMNAYWEPLVMQLPVLPNGKHW
                     HVDTNTNAEYFDGEDFTAKTEFLGVNTIRVPARTTIILVAE"
     gene            complement(2045..2806)
                     /locus_tag="DHV81_06145"
     CDS             complement(2045..2806)
                     /locus_tag="DHV81_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCI94046.1"
                     /translation="MTNQTNHTNHINKTAIITGASRGIGAAIAKKLASCGYNLSLCCR
                     NSSDRLNTLADELHCAYGIEVLCYTGNVGDFFFAKDMVTKTISHFGHVDVLINNAGIS
                     HIGLLSDMTPEEWNNIVEINLTGVFNFTKLVIPYMVADKCGRILQISSVWGNVGASCE
                     VAYSACKGGINAFTKALGKELAPSHIPVNAIACGVIDTDMNRCFDETERAELADEIPA
                     GRFATACEVADMAYSVITAPDYLTGQIITFDGGWI"
     gene            3063..3136
                     /locus_tag="DHV81_06150"
     tRNA            3063..3136
                     /locus_tag="DHV81_06150"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:3097..3099,aa:Pro,seq:cgg)
     gene            3346..5235
                     /locus_tag="DHV81_06155"
     CDS             3346..5235
                     /locus_tag="DHV81_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCI94047.1"
                     /translation="MNIVSQMCAIAVMFVIMYFYISQKKIILHTSKAFVEVWIAGMLS
                     LFSDIFVLVAIEKRSGYPLTATNIICKLYLWTVTLVAVVAFWYICVDIFRKKELERKW
                     RKRLWIFGISIDILIMVVPIKIYDSDNIVYTYGPAVIITYVVTVILLALILLLTKKYS
                     QAINPRRRKSMQVWVALMFISAVIQFIDKKILIVSFASVIGVLILFIMLENPMANIDR
                     DTGFFNLNAFFEYMKEAYGQGNDVSIVCIRYGSNGNDFFTREMEKSIFDEVVSFINKL
                     PDNYVFRSSANEYLLVFENTDCAEKTIAILERRFDKPWGGDNMTMLFGEIYYLRSTEL
                     VRRPSDILGLFQYAKRNRAEFTGRGVMLINNEVIEHIYDENSVENEIIEALRDNRVEV
                     FYQPIYNTKTHKFTSAEALVRIRSREGNIIPPGRFIAVAEKRGLILRLGERVFEKVCR
                     FIVQHDIHAMGIEYIECNLSVVQCAYDHLAQDFIAIMEKYHVDANDIVLEITESASIT
                     EKKILLDNMNALRKVGVRFALDDFGTGQSNLSYIVDMPIDIVKFDRGMTNAYFDNGKG
                     KPVMDAAMGMIQKLKLEIVSEGIEEKEQLAKLDELGIDYIQGYYFSKPRNAAEFISFI
                     ESNNA"
     gene            complement(5709..5939)
                     /locus_tag="DHV81_06160"
     CDS             complement(5709..5939)
                     /locus_tag="DHV81_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94048.1"
                     /translation="MLFWGFLRFQLLFLLFSGFLAGVWDAVLGISAISATFSCSFPAF
                     WAGLGCCLGNFCGFSYFFLLFSSFLAGFGVLF"
     gene            6222..6959
                     /locus_tag="DHV81_06165"
     CDS             6222..6959
                     /locus_tag="DHV81_06165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family DNA-binding protein"
                     /protein_id="HCI94049.1"
                     /translation="MEGEILEIQERLETITEEGTIYPTQKDGNVKWYVWKNGRREYLS
                     KKNDKEIRNLVNKKYLELYLKDTINELRLLETNRKARKKYKTDYAQKMLKQKPYSSIL
                     LAVDKKENEEANEEANEKSQVPNPEALKFYTKMGIIVRSKSEVIIATALYDNNIKFEY
                     EKAIKISDGIYYPDFTVEKKNGDIILWEHLGLIDNPDYRNKAYRKILKYNENGYYQGK
                     NLILTYETAESPLDPMDVEHEIEKMCR"
     gene            7409..8290
                     /locus_tag="DHV81_06170"
     CDS             7409..8290
                     /locus_tag="DHV81_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI94050.1"
                     /translation="MKNRMKKLLVLATAATMMTAAFTGCGSSSDGADNNADTSADEGT
                     SNENLSGSLSLAGSTSMEKLCEALKESFMEKNPGVTVTVEYTGSGSGIESVTAGSVDI
                     GDSSRALTDDEKANGVEENIVAIDGIAVITDKDNSVTELTSDDLKKIYTGEISNWKDL
                     GGKDEAIVAIGREAASGTRGAFEELLDVKDQCKYAQELDSTGAVLAKVGSTPGAIGYV
                     SLDVLDDTVTAMIIDGVEATEENIVAGKYLLSRPFVMATKGKIDEQNDIVKAWFDYVN
                     SDEGQAVIKKVGLILPQ"
     gene            8482..9384
                     /gene="pstC"
                     /locus_tag="DHV81_06175"
     CDS             8482..9384
                     /gene="pstC"
                     /locus_tag="DHV81_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCI94051.1"
                     /translation="MKHKSIVENVAKIIFTVCAVVAIFAVLSITIYMFLKGAPAFFKV
                     GVLNLLFGTKWAPTAADPSYGILYIILTSIIGTAVSILIGVPIALLTAVFLTEVSNKK
                     LSAVVQPAVELLAAIPSVIYGLLGLMILNPVLYKLEKHIFANSATHQFTGGSNLLAAI
                     IVLAIMILPTVINMSVSSIQAVPQQMRAASLALGATKIQTIFKVTVPAAKSGIMTGIV
                     LGIGRALGEAMAINMVAGGTVSFPLPFNSVRFLTTQLVSEMGYASGLHRQVLFSVGLV
                     LFVFIMIINLTLMKLKKKGAVEND"
     gene            9377..>10189
                     /gene="pstA"
                     /locus_tag="DHV81_06180"
     CDS             9377..>10189
                     /gene="pstA"
                     /locus_tag="DHV81_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PtsA"
                     /protein_id="HCI94052.1"
                     /translation="MTSINGKRRRIGDDVVYFFIYLCAALSVVLLAGIIIYVFVRGIG
                     QVNWQFLTSETSVLRGTVGIAGNLLNTLYIIIITMLIATPVGVGAAVYLNEYAKPGKL
                     VSTIEFATETLAGIPSIIFGLFGMMFFGQTIGFGYSILTGSLTLTLMVLPLITRNTQE
                     ALKTVPDSYRHGAVGLGAGKWHTIRTILLPSAMPGIITGVILAIGRIVGESAALLFTA
                     GSAGMLPKVASGYFSKIMQSGGTLTIKLYLAATSEGKFDQAFGIAVVLLVIVF"
BASE COUNT         3248 a         1879 c         2328 g         2734 t
ORIGIN      
        1 atgccgattt ttgagtttga tgagatggag agcgcgagag tggtcgatgg tgtgcagctt
       61 tacaattact ggggctacaa cacagtatca ttttttgcac cgaataccag ctatgccttc
      121 aacgaggagc acaaccacga gggagacgag ctgaaatcgc tgataaaggc tttaaaggaa
      181 aatggcatag aggtcattct tgatgtggtg ttcaaccata ccgctgaggg aaatgagatg
      241 ggaccgtgct tctccttcaa gggaatagac aataacgtgt actatatgct tacaccggat
      301 gcacactact acaatttcag tggctgtgga aacgttatga actgtaatca tcctgttgta
      361 cgcagcttta tcatagactg tctgagacac tgggccattg agtacagagt ggatggcttc
      421 agatttgacc tggcttccat tctcggaaga gaccaaaacg gtgcacctat ggccaatccg
      481 ccgattcttg agtcgctggc attcgacccg gtacttggca agatgaagct catcgcagag
      541 gcctgggatg caggcggact gtatcaggtg ggaagcttcc cgtcatggaa cagatgggct
      601 gagtggaacg gcagatacag ggatgacatg agaagctttt taaagggcga tgacggtatg
      661 gcgggaaatg ccatcacacg tatcaccggc tcacgcgacc tttacagccc ggagagcaga
      721 ggacacaagg catcagtcaa tttcatgacc tgtcatgatg gctttacact gtatgatctt
      781 tactcataca atgaaaagca caatgagaaa aacggctgga ataacacaga cggagacaac
      841 aacggacata gctggaactg tggcgcagag ggcgagacag acgatccaaa tgtaaacgga
      901 cttagaaggc gccttataaa gaatgcattt gcagcgcttt tgtgcagccg tggcccggca
      961 atgttttttg cgggcgatga gttctgcaac acccagtttg gaaacaacaa cgcgtattgt
     1021 caggacaata tcatctcatg gctcgactgg agcaggcttg aggaattcaa ggaaatccat
     1081 gatttcgtgc gtcatatgat tcagttcagg aaagagcatc ctatactcag aaagatgaca
     1141 aagccttcat cctgccagtt tccggaaatc agtgtgcaca acggtacacc gttcaatgcc
     1201 tcaacggatt acaagacaaa gcttatcggc atcatgtatg ccggaagaaa tgaggaggac
     1261 aaggaggatg atattgtatt ctattgcatg aatgcatatt gggagccgct tgtgatgcag
     1321 cttccggtgc ttccgaacgg aaaacactgg catgtggaca ccaacaccaa tgcagagtat
     1381 tttgacggag aggacttcac ggcaaagaca gagtttttgg gtgtcaacac cataagagtt
     1441 cctgcaagga ccacgataat acttgtggca gaataacaat aaaatctgtt gcctgaatgc
     1501 aattcgatgt atacttttat attgtaaaaa acagcccggc acacattgtg atatgtgcac
     1561 cgggctgttt ttcgttacag ttttggctta gactgtaaca tttccccctt tcatttttca
     1621 atatatttta tgtgacattt gcatcagcag ccggtgatgg ttgtgaagtg ataccggaag
     1681 gccttgtata tatcacataa tcagttgtga gttggacaaa tttcgtctgt gaatccaaga
     1741 aaataattgg gtgtgacacc aaaaattgta cttaaatggc aaatgacaaa aatgtcaggc
     1801 actctacggc ctgtcctgta gcctgaaata gttgattctg cacagcacat gatttcagaa
     1861 agagcaatgt tggtcaagtt gtggagcctc atcagataat caagcctgtc aggcacagtt
     1921 gtactaatgg ttgatagttg cttttctaat agaaagttat ttatagtact cataaatgaa
     1981 ttgtaacaca ataattacaa aaatcaacaa atatctgaaa atacgaaaaa ttaaaattac
     2041 aattttaaat ccagccacca tcaaaagtga ttatctgtcc tgtgaggtaa tcaggtgcag
     2101 tgatgacgga gtaagccata tctgcgacct cacaggctgt ggcaaagcgt ccggcaggaa
     2161 tttcatcggc cagctcagcg cgttctgtct catcaaagca gcggttcata tcggtatcta
     2221 tgacaccgca ggctatggca tttaccggaa tatgcgatgg cgcaagctcc ttgccgagtg
     2281 ccttggtaaa tgcatttata ccgcctttgc aggctgagta tgccacctcg caggatgctc
     2341 cgacgttgcc ccagacagag gaaatctgta gtatacggcc acatttatcg gcaaccatgt
     2401 aagggatgac gagctttgta aagttgaaaa caccggtcag atttatctcc acgatattgt
     2461 tccattcctc cggtgtcatg tcgctcagca gcccgatatg cgatataccg gcattgttaa
     2521 tgagcacatc tacatggcca aaatgggaaa tggttttcgt aaccatatcc ttggcaaaga
     2581 aaaagtctcc tacatttccg gtgtagcata gcacctcaat tccgtatgca cagtgaagct
     2641 catcagcaag tgtatttagc ctgtcagatg aattgcggca gcaaagtgag aggttatagc
     2701 cacaggaagc cagctttttg gcaatggcgg caccgatacc gcgtgaagct ccggttatta
     2761 tggctgtttt atttatgtga tttgtgtgat ttgtctgatt tgtcatgata ttctccttat
     2821 aatttatata ccgtggtgct aagccggtcg ctgcgctgcc gggccggctt catagtataa
     2881 agaaaataat tacgtgatta aattacagtt cagtcatggg tatgatacct agctgtgagg
     2941 ctgggcacaa tagtgtaaat ttttatctta tacagcataa atcataccat aaatatatat
     3001 tatggggtag acaaaattta tacattgtga tattatttaa tagttgcaaa acaaattgca
     3061 gacggcgtgt ggctcagttt ggtagagcgc tacgttcggg acgtagaggt cgcaagttcg
     3121 aatcttgtca cgccgattta tttatgtatg tatttgaata gttaccgaat gtattattat
     3181 ggagcacgcg tatagcgtgt ttttttgtta ctgttcagtg tgaatagtaa cttttttatt
     3241 taattctttg taaatcaaaa taatatattg ctttattggt aaaataacgg taaaatagta
     3301 ctagtgtatt ggcttgattt aggaaaggta caggaaatag aggatatgaa tattgtttca
     3361 caaatgtgtg ccattgcggt gatgttcgta ataatgtatt tttatatttc gcagaaaaag
     3421 attatattac atacatcgaa ggcttttgtt gaggtatgga ttgcaggcat gctgtcactt
     3481 ttctctgata ttttcgtact tgttgcaatt gaaaaaagaa gcggatatcc tcttactgct
     3541 accaatatta tctgcaaatt atatctctgg acggttacat tggttgccgt agtggccttc
     3601 tggtatattt gtgtggatat tttcaggaaa aaagagcttg agcgcaagtg gagaaagcgg
     3661 ctgtggatat ttggaatctc cattgatatt ctgataatgg tcgtaccgat taaaatttat
     3721 gattcggata atatagttta tacatatggt ccggctgtta ttataacata tgtagtaact
     3781 gtaattctgc tggcgcttat tctactgctg acaaaaaaat acagtcaggc tataaatccg
     3841 agaagaagaa agagcatgca ggtctgggtg gctcttatgt ttatatcggc ggttattcag
     3901 tttatagata aaaaaatatt gattgtcagc tttgcgagtg tgataggggt gcttatcctt
     3961 tttattatgc ttgaaaatcc gatggcaaat attgacaggg atacaggttt tttcaacctc
     4021 aatgcctttt ttgaatacat gaaggaggca tatggacagg ggaatgatgt atcgatagtg
     4081 tgtattcgct atggaagcaa tggaaatgat tttttcaccc gcgagatgga aaaaagtatt
     4141 tttgatgagg tggtttcttt tataaataag ctgcctgata attatgtttt tagaagctcg
     4201 gcaaacgagt atctgctggt ttttgagaat actgattgtg cagaaaaaac aatcgcaata
     4261 ttggaacgca ggttcgacaa gccatggggc ggtgacaata tgacgatgct gtttggggag
     4321 atatattatc taaggtctac tgagcttgtg cggagaccat cggatatact ggggcttttt
     4381 caatatgcaa agcgtaacag agctgagttt acggggcgcg gcgtgatgct cataaataat
     4441 gaagtcattg agcatatata tgatgaaaat tcggttgaga atgagataat agaggcattg
     4501 cgcgataacc gtgttgaggt attttaccag cctatttata acactaaaac acataaattc
     4561 acctctgcgg aggcgcttgt cagaataagg tcgcgcgagg gaaatatcat acctccgggc
     4621 aggtttattg ctgtagctga gaagcgggga ctcattttaa ggcttggaga gcgcgtgttt
     4681 gagaaagtgt gcagatttat tgtacagcat gatatacatg cgatgggaat tgagtatatt
     4741 gaatgcaacc tgtcagtggt gcagtgtgct tatgaccatt tagcgcagga ttttattgca
     4801 attatggaga aatatcatgt ggatgcaaat gatattgtgc ttgagattac agagtctgca
     4861 tctatcactg aaaaaaagat actgcttgat aatatgaatg ccctgcgtaa ggtgggagtg
     4921 aggtttgcgc tggatgattt tggcacaggt cagtcaaatt taagctatat tgttgatatg
     4981 ccgattgaca ttgtgaagtt tgacagaggc atgacaaatg catattttga caacggaaag
     5041 ggcaagcctg ttatggatgc cgcaatgggc atgattcaaa agctaaagct tgagattgtt
     5101 tctgagggaa tcgaggagaa ggagcagttg gcaaaactgg atgagcttgg aatagattac
     5161 attcagggct actatttctc aaaaccgaga aatgctgctg agtttatttc attcatagaa
     5221 agtaataatg catagtttat ttcattcata gaaagtaata atgcatagtt tattttattc
     5281 atacaagata ataatgatat gaggggcaaa aggtattttg cccctcattt atgattaatc
     5341 agattgctac atgctacgca tttccataat ctgactacac ctcgaagtcc gctaattttc
     5401 tgcgtccccg cagcaaactg ttactttctc ggtgtagtgc gggtcactaa ggtatacatc
     5461 gaattgcatg caagcgtgca tcgatgatac cttttgaccc tttaatcata atatttgggt
     5521 agaaatgttt ccatatggca ggggaacata tgaatcttga gcaataatta tttttatttt
     5581 ttctgaaaat tcattttttt tgtcttttta tttagaaaat actggatttt taaattatca
     5641 taaggtacaa tagccatagg ttggtagcat agtaagcggg tcttgtggag ctgtaattcc
     5701 taaaatttct aaaacagcac cccaaaccca gccaaaaagc ttgaaaagag caggaaaaag
     5761 tagctgaaac cgcagaaatt ccccaaacag catcccaaac ccgcccaaaa agctggaaaa
     5821 gagcaggaaa aagtagctga aatcgcagaa atccccaaaa cagcatccca aaccccagcc
     5881 aaaaatccgg aaaagagcag aaaaagtagc tgaaatcgca gaaatcccca aaacagcacc
     5941 ccaaaccccg cccaaaaagc cagaaaagag caggaaaaag tagctgaaat ccattaaaat
     6001 cataaaacag ctccaaacta tagtcggttt ctatatttat atggtgctat caaacaaatg
     6061 gagaccataa tctccacttt tgaagataaa tgacagcttt catttatcat attccccaca
     6121 aaaacaattg aattactgtt agaatcacat acatggcatc acatgatgca aagagttagg
     6181 aggaaaataa aataagttca atagataata gaatccaatg gttggaaggt gagatattag
     6241 agattcagga aaggctggag actatcactg aggaagggac aatctatcca acacaaaagg
     6301 atggaaatgt taaatggtat gtttggaaaa atggcagaag agagtattta tcaaagaaaa
     6361 atgataaaga aatcagaaat cttgtcaata aaaaatatct ggagctttat ctgaaggaca
     6421 caataaatga actaagacta ctcgagacaa acaggaaggc ccggaaaaaa tataaaacgg
     6481 attatgccca gaaaatgttg aagcaaaaac cttatagcag tatactttta gccgttgata
     6541 agaaggaaaa tgaagaggca aatgaagagg caaatgaaaa gtcacaggtt ccaaaccctg
     6601 aagctctcaa gttttatact aaaatgggaa ttatagtgcg gtcaaaatca gaagtaatta
     6661 ttgcgacagc cttgtatgat aataatataa agtttgagta cgagaaggcg attaaaatct
     6721 cagatggtat ttattatccc gattttacgg ttgagaagaa aaatggcgac ataattctgt
     6781 gggagcatct tggcctgata gacaatccag attatcgaaa taaagcttat cgaaagatat
     6841 tgaaatataa cgaaaatgga tattatcagg gaaaaaatct tatattgaca tatgaaacag
     6901 cggaaagtcc attggacccc atggacgtag agcatgaaat agaaaaaatg tgcaggtgat
     6961 aaaatgctca aaaaattacg aagaaaattt atagtcatag ctatgctatg agtttcaatt
     7021 gttcttattg caattgtagg cacgataaac atcgcaaact atataaaagt acaaacgagg
     7081 cgttggatgc atgtcttaag ctgattgacg gaaacggagg aacattcccg gatcttttag
     7141 atacagaaaa cgcaaacaca gatacattcg gcaatacagc agatgaaagt acaaataaag
     7201 ggactcccac gctcaaagag ccaccatccg gcagaacaga tgtatagcca ccggaggata
     7261 taaatttata atcgtattgt agattaaaaa tattttttta catgcatttt acacactaat
     7321 gattttgcat tttacatttc cccattacta tatcagttgt aacaaacaaa gaaacaaatt
     7381 caagaaagta atgaaagagg aaattaaaat gaaaaatcgt atgaaaaaac ttttagtttt
     7441 agcaactgct gcaacaatga tgacagcagc atttacagga tgtggttcaa gctcagacgg
     7501 agcagacaac aatgcagata caagcgcaga tgaaggtaca tcaaatgaga acttaagcgg
     7561 ttcactttca cttgcaggaa gtacttcaat ggagaagctt tgtgaggcgc tcaaggagtc
     7621 attcatggag aaaaaccctg gagtaacagt aacagtagag tatacaggct ctggttcagg
     7681 aatcgagtca gtaacagcag gaagcgtaga tatcggagat tcatcaagag cacttacaga
     7741 tgatgagaaa gcaaacggcg ttgaggaaaa catcgtagca atcgacggaa tcgcagtaat
     7801 cacagacaag gacaactcag taacagagct tacatcagat gacttaaaga aaatctacac
     7861 aggtgagatt tcaaactgga aggaccttgg tggaaaagat gaggcaatcg tagcaatcgg
     7921 ccgtgaggca gcttctggta caagaggagc cttcgaggag cttcttgatg taaaggatca
     7981 gtgcaaatat gcacaggagc ttgactcaac aggagcagtt cttgcaaagg taggctcaac
     8041 accgggagca atcggatatg tttcacttga cgtgcttgat gatacagtaa cagcaatgat
     8101 aattgacgga gtagaggcaa cagaggaaaa cattgtagca ggcaaatatc tcctttctcg
     8161 tccattcgta atggctacaa agggcaagat tgacgagcag aacgatattg ttaaagcttg
     8221 gtttgactat gtaaactcag atgagggaca ggcagttatc aagaaggtag gtcttatcct
     8281 ccctcagtag aaattatcgg gcatattaat gatacgaaat acagacatcg atacgagaca
     8341 tcgctagtaa gtataagtat aaaaaataca aaatacagac atcgatatta gacatcagta
     8401 ttaatacata tcgacaagat acagggtttc ctcaggaaat ggcaggtttc ctggggaact
     8461 ttttgaaaca aggagtatca aatgaaacat aaatccatag tagagaatgt tgccaaaata
     8521 atattcacgg tatgtgcggt tgtggcaata ttcgcagtat tatcaataac aatttatatg
     8581 ttccttaaag gcgcaccggc atttttcaag gtcggagtgt taaacctctt attcggaaca
     8641 aaatgggcgc cgacagcggc agacccaagc tacggaatat tgtacataat cctcacatcc
     8701 attataggaa cagcagtatc tatactcata ggagtaccga ttgctcttct tacggcagta
     8761 tttctcactg aggtgtcaaa caaaaagctc tcagcggtag tacagccggc agtagagctg
     8821 cttgcagcca ttccatcagt aatctatgga cttttgggac ttatgatact taatccggta
     8881 ttatacaagc tggagaaaca tatttttgca aactcagcaa cacatcagtt taccggcgga
     8941 tcaaatctgc ttgcagcaat catagtgctt gcaatcatga ttcttcctac ggtaatcaac
     9001 atgagcgtat catcaataca ggcagtaccg cagcagatga gagcggcatc acttgctctt
     9061 ggagcaacaa agatacagac aatatttaaa gtgacggttc ctgcagcaaa gtcaggaatc
     9121 atgacaggaa ttgtgctcgg aataggtcgt gcgctcggag aagcgatggc tatcaacatg
     9181 gtagccggtg gaacggtaag ctttccactt ccgttcaact cagtgcgttt ccttaccaca
     9241 cagctcgtca gcgagatggg atacgccagc ggacttcaca gacaggtgct tttctcagta
     9301 ggtcttgtgc tttttgtgtt tatcatgata atcaacctca cccttatgaa actcaaaaag
     9361 aaaggagctg tggaaaatga ctagtataaa tggaaaacgc cgcagaatag gcgatgatgt
     9421 agtatatttt ttcatatatc tgtgcgcagc attatcggta gtgctgcttg caggaatcat
     9481 catatatgta tttgtcaggg gaatcggcca ggtaaactgg cagtttctga ccagcgagac
     9541 atctgtatta agaggcaccg tcggaatagc gggcaacctg ctcaatacac tttacatcat
     9601 aattatcacc atgctcatag caactcccgt aggagtggga gcagccgtgt acttgaatga
     9661 gtatgcaaag ccgggtaagc tcgtatccac aatagagttt gccacagaga cacttgcagg
     9721 aataccgtca atcatctttg gccttttcgg aatgatgttc tttggacaga caataggctt
     9781 tggctacagt atcctgacag gctcactgac actgacactc atggttctgc ctctcatcac
     9841 aagaaataca caggaggcgc ttaaaactgt accggacagc tacagacacg gagcagtcgg
     9901 acttggagcc ggaaaatggc ataccatccg caccatactg ctgccgtcag ccatgccggg
     9961 aatcatcaca ggtgttatcc ttgcaatcgg ccgaatagta ggtgagtcag cagcacttct
    10021 ctttacagca ggaagcgcag gaatgcttcc aaaggttgcc agcggatatt tctcaaagat
    10081 aatgcagtca ggtggaacac ttaccatcaa gctctacctt gcagcgacaa gtgaaggaaa
    10141 attcgaccag gcatttggaa ttgcggttgt gcttctcgta atcgtattc
//
LOCUS       DPUF01000173            6510 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1656, whole
            genome shotgun sequence.
ACCESSION   DPUF01000173 DPUF01000000
VERSION     DPUF01000173.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1656"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="DHV81_06185"
     CDS             complement(<1..121)
                     /locus_tag="DHV81_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94053.1"
                     /translation="MAKSLIDIVKEQQAKLGYRKEIVRLYYPLSTLRHFFECAG"
     gene            complement(280..1596)
                     /gene="hydF"
                     /locus_tag="DHV81_06190"
     CDS             complement(280..1596)
                     /gene="hydF"
                     /locus_tag="DHV81_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HCI94054.1"
                     /translation="MGNLNETPSANRLHIGIYGKTNSGKSSLINAVTKQEVSIVADVA
                     GTTTDPVYKPMEIHPLGPCVIIDTAGFDDDSELGERRVEKTHLAAEKTDIAVVVLDIA
                     EVIAAKKAGVPFKKAFKDEAEWSLFFAKKHTPVVFALNKIDEILLSAEAQEKSRGKLD
                     NAAIEAAKCWVYEENSAVMGKNADNSFEVVAVSALKGKGMDAVISALTRLLPEDFGQE
                     FILGDLVCETDLVLLVMPQDIQAPKGRLILPQVQTLRELLDRKCLVMSTTTDKMTDAL
                     AALSHAPKLIVTDSQVFGYVYEHKPAESMLTSFSVLFAAYKGDLPYYVESARAIDALK
                     PSSRVLIAECCTHAPLAEDIGRVKLPNLLRKRVGEELTVDITSGTDFPDNLSQYDLVI
                     QCGACMFNRKYVLSRIDRARTQHVPMTNYGVAIAHLTGILDKVSMP"
     gene            complement(1607..3034)
                     /gene="hydG"
                     /locus_tag="DHV81_06195"
     CDS             complement(1607..3034)
                     /gene="hydG"
                     /locus_tag="DHV81_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HCI94055.1"
                     /translation="MYNVKSLKAEEFISDEEIKETLEYAEANKNNIGLIDAIIEKAKL
                     KKGLTHREASVLLACEIPEKLDEVYKLAQQIKKDFYGNRIVLFAPLYLSNYCVNGCVY
                     CPYHMKNKHIARKKLTQEEIVKEVTALQDMGHKRLAIEAGEDPVNNPIEYILECINTI
                     YSIKHKNGAIRRVNVNIAATTVENYRKLKEAGIGTYILFQETYHKESYEQLHPTGPKH
                     DYAYHTEAMDRAMEGGIDDVGLGVLFGLDKYKYEFAGLLMHAEHLEAVHGVGPHTISV
                     PRIKHADDIDPDVFDNGISDDTFAKICALIRISVPYTGMIISTRESQTVREKVLLLGV
                     SQISGASKTSVGGYADPEAEKNAEATSEQFDVSDQRTLDEVVNWLIKMDYIPSFCTAC
                     YREGRTGDRFMALCKSMQILNCCHPNALMTLKEYLEDYASPQTRELGMKLIDREIENI
                     TNPKVKQTCYEHIHDIADGKRDFRF"
     gene            complement(3091..4149)
                     /gene="hydE"
                     /locus_tag="DHV81_06200"
     CDS             complement(3091..4149)
                     /gene="hydE"
                     /locus_tag="DHV81_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HCI94056.1"
                     /translation="MTENTAIIKIIDEITACRNITLEQLHMLLETDDMQAVDYLRKRA
                     SEKAHETYGNQVFIRGLIEFTNYCKNDCLYCGIRHSNTHADRYRLSTEQIMACCESGY
                     ELGFRTFVLQGGEDPYYTDERICEIVSGIKAKYPDCAVTLSIGEKSKESYQSYFDAGA
                     DRYLLRHETADEKHYSRLHPAEMSLANRKQCLWDLKEIGYQVGCGFMVGSPGQTVETL
                     YEDLQFIKELEPHMVGIGPFISQKDTPFANEKSGTMDETLRLLSIIRLIHPKVLLPAT
                     TALGTIHPLGREKGIQSGANVVMPNLSPVNVRDKYKLYDNKICTGDEAAECRFCMENR
                     MKSIGYQVVVSRGDYADF"
     gene            complement(4175..4420)
                     /locus_tag="DHV81_06205"
     CDS             complement(4175..4420)
                     /locus_tag="DHV81_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HCI94057.1"
                     /translation="METRIALIGIIVENPDSVEQLNHLLHEYGSFIVGRMGIPYEKKN
                     VNIISVAIDAPQDIISALSGRIGRLDGIGVKTVYSNK"
     gene            5251..5772
                     /locus_tag="DHV81_06210"
     CDS             5251..5772
                     /locus_tag="DHV81_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94058.1"
                     /translation="MKKILVLPLLLCLIFCSFLSLDKNLDEFDPDRTAYGSYDHRVVW
                     GSYASSDKAALPQQEVLDSRAVLYSESDMSALMDCQYIEEIPSFPEVICRIRTSSLGF
                     LGRLTSNLSWLGALLQLIHIILFAYAAIVVMRICLTNTSRRFIIRFIHDKDGHKLISF
                     ADYFKTRAYAEEI"
     gene            5837..6028
                     /locus_tag="DHV81_06215"
     CDS             5837..6028
                     /locus_tag="DHV81_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94059.1"
                     /translation="MLEAIIIVVCAAVCIGAAAYAWWMENGPEKDDSDAAGKNSQGHA
                     YTKYDSNIKDDIVFHKAHK"
     gene            complement(6208..>6510)
                     /gene="pgi"
                     /locus_tag="DHV81_06220"
     CDS             complement(6208..>6510)
                     /gene="pgi"
                     /locus_tag="DHV81_06220"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428043.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCI94060.1"
                     /translation="LCANVAAQIVAFACGKADDNKNKNFEGGRPSSIIIGDQVNPASL
                     GALLAHFENKIMFQGFLWNVNSFDQEGVQLGKLLAKKVLAHETDGALKELSDMLNI"
BASE COUNT         1612 a         1610 c         1303 g         1985 t
ORIGIN      
        1 cccctgcaca ctcaaagaaa tgtctgagcg tggaaagagg ataatacagt ctgactattt
       61 ccttcctgta gccaagcttc gcctgctgct cttttactat atcaataaga ctcttcgcca
      121 gacgagcgta accggtggcg ctataatcgc tgctgtaatt gtctctataa tcgccgctgt
      181 aattgccttt ataatcgcca ctataattca ctttgtcgtc aaaatcgctt gttttcgtca
      241 taccgatagc ttctcttcct cccggctttt aaggtttttc tagggcatgc tcaccttatc
      301 gagtattcct gtcaggtgtg ctatagccac gccatagttt gtcatcggca catgctgagt
      361 gcgtgccctg tctattcttg ataatacata ttttctgtta aacatgcaag caccgcactg
      421 aataacgaga tcgtactgag aaagattatc agggaagtct gtaccgcttg taatatccac
      481 ggtaagctcc tcaccgacac gctttcgaag caggttcgga agcttcaccc tgcctatatc
      541 ctctgcaaga ggcgcatgtg tacagcactc tgcgataagc actcttgagg atggctttaa
      601 ggcatcaatt gcccttgcgc tctccacata atatggcaga tcacccttgt atgctgcaaa
      661 aagcactgaa aatgatgtga gcatactctc cgccggtttg tgctcgtaca catagccaaa
      721 cacctgtgaa tctgtcacga tgagctttgg cgcgtgagat aatgctgcaa gcgcatctgt
      781 cattttgtca gtcgtcgtgc tcatcacaag acattttcta tcgagcaatt ccctgagtgt
      841 ctgcacctgt ggcaatatga gtctgccctt tggcgcctgg atatcctgcg gcatgacaag
      901 aagcaccagg tctgtctcac aaacaaggtc tcccaggata aattcctgtc cgaaatcctc
      961 aggcaaaagg cgtgtgagtg cagagataac ggcatccatt cccttgccct ttaatgcgct
     1021 gactgctaca acctcaaagc tattgtctgc atttttgccc atcacagcac tgttttcctc
     1081 ataaacccag cactttgcgg cctctattgc agcattgtca agctttcccc tgctcttttc
     1141 ctgtgcctct gcggaaagca aaatctcgtc tatcttgttt aaggcaaata caaccggagt
     1201 atgttttttt gcaaaaaaca ggctccactc tgcctcatcc ttaaaagcct ttttaaacgg
     1261 tacccctgcc ttttttgcag caatgacctc tgcaatatca agcactacaa ccgctatatc
     1321 tgtcttctct gccgccagat gagtcttttc cactcgtctt tcaccaagct cactgtcatc
     1381 atcaaagccc gccgtatcaa taatcacgca tggtccgagc ggatgtatct ccatcggctt
     1441 gtacacaggg tcggttgtcg tacctgccac gtcagctaca atggaaacct cctgctttgt
     1501 aaccgcattt atcagagatg acttaccact gtttgtcttt ccgtatatac ctatatgaag
     1561 tctgttggca gaaggcgtct cattcaaatt tcccatattt cctcctttag aaacggaaat
     1621 cacgctttcc gtctgcaata tcatgtatat gctcatagca ggtctgctta acctttggat
     1681 tggtaatatt ttctatttcc ctatcgatga gcttcattcc aagctctctt gtctgcgggc
     1741 ttgcatagtc ctcgagatac tccttaagtg tcatgagtgc gttcggatga cagcagttaa
     1801 gtatctgcat gctcttgcac agtgccataa aacggtctcc cgttctgcct tctctgtagc
     1861 aggctgtaca gaaggacgga atatagtcca tctttataag ccagtttacc acctcgtcaa
     1921 gtgttctctg gtcgctgaca tcaaactgct ccgatgttgc ctctgcattt ttctcagcct
     1981 ccgggtctgc atagcctcca acacttgtct ttgatgcgcc gctgatctgt gaaacaccga
     2041 gcagcagaac cttttcacgg acagtctggc tctcacgggt tgagattatc attcctgtgt
     2101 atggcactga aatacggata agtgcgcata ttttggcgaa ggtatcatca ctgatgccat
     2161 tgtcaaatac atccgggtct atatcatctg cgtgctttat acgcggcaca ctgatggtgt
     2221 gaggacctac accgtgcact gcctcaaggt gctctgcatg catcaaaagt cctgcaaact
     2281 cgtacttgta tttgtccagt ccgaaaagta caccaagtcc cacatcgtca atgccgccct
     2341 ccatagcacg gtccattgcc tctgtgtgat atgcatagtc atgttttggt cctgtcggat
     2401 gaagctgctc atagctctcc ttgtgatatg tctcctggaa gagtatgtag gttccgattc
     2461 ctgcctcctt gagctttctg taattctcca ctgtagtggc agcgatattt acatttacac
     2521 ggcggatagc tccgtttttg tgcttgatgc tgtagattgt gttgatacac tcgagaatat
     2581 actcaatcgg attgtttaca gggtcctctc ctgcctcgat tgcaagacgc ttgtgtccca
     2641 tatcctgaag ggctgtgacc tcctttacta tctcctcctg agtgagcttc tttctcgcaa
     2701 tatgcttatt tttcatgtga tatggacagt aaacacagcc gttcacacag tagtttgaca
     2761 gataaagcgg tgcaaaaagc acgattctgt ttccgtagaa atccttcttt atctgctgtg
     2821 caagcttgta aacctcgtca agcttctccg gaatctcaca ggctaaaagc actgatgcct
     2881 ctctgtgtgt gagtcctttt tttagctttg ctttctcgat aatcgcatcg ataagcccta
     2941 tattattttt gtttgcctct gcgtattcga gagtctcttt gatttcctcg tctgagatga
     3001 attcctcagc ctttaatgat tttacgttgt acatttttat ctccttctat atcctagtgc
     3061 tgttttatat ttattaatcg taactattaa tcaaaaatct gcgtagtccc cgcgcgacac
     3121 caccacctga tagccgatgc ttttcatccg gttttccatg cagaagcggc actctgcagc
     3181 ctcatctccg gtgcagattt tattgtcgta aagcttgtat ttgtcacgga cattgaccgg
     3241 tgagaggttt ggcatcacga cattggcacc cgactgtata cccttttccc tgccgagcgg
     3301 atggattgtg ccaagcgctg tcgtggcagg cagcagcact tttggatgta tgagcctgat
     3361 gattgaaagc aggcgaagcg tctcatccat cgtgcctgac ttttcgtttg caaaaggtgt
     3421 atccttctgc gatatgaacg gaccgattcc aaccatgtga ggctcaagct cctttataaa
     3481 ctgcaaatcc tcgtacaagg tctccactgt ctgtcccggc gagccgacca tgaagccgca
     3541 gcccacctga tatcctatct ctttcaaatc ccaaaggcac tgctttctgt ttgcaagtga
     3601 catctctgcc ggatgaagcc tactgtaatg cttttcatcc gcagtctcat ggcgcagcag
     3661 atatctgtct gcccctgcat caaaatagct ctgataactc tcctttgatt tttctccgat
     3721 agagagtgta actgcgcagt ccggatactt tgccttaatc ccggacacta tctcacagat
     3781 gcgctcatcc gtgtagtacg gatcttcacc tccctgcagc acaaaggtac gaaaaccaag
     3841 ctcatagccg ctctcgcagc atgccataat ctgctccgta ctcaggcggt agcggtccgc
     3901 atgggtattg ctgtgcctta taccgcagta gagacagtcg tttttgcagt aattggtaaa
     3961 ctctatcagc cctcgtataa aaacctgatt gccataggtc tcgtgtgctt tctcagatgc
     4021 cctctttcta aggtaatcaa ctgcctgcat atcatccgtc tcaagcagca tatgcagctg
     4081 ctcgagggtg atattccggc aggccgtaat ttcatctata atttttatta ttgctgtatt
     4141 ttctgtcatg ctttctcctg tagtctggtc tgctttattt attagagtaa actgtcttca
     4201 ctcctatgcc atccagcctg ccaatcctgc cggacaaagc actgataata tcctgtggtg
     4261 cgtcaatcgc cacactgatg atattcacgt tctttttctc gtatgggata cccatgcgcc
     4321 caacaataaa gctgccgtac tcatgcagca gatgattaag ctgctcaacc gaatccgggt
     4381 tttcaactat aatgccgatc agggctattc ttgtttccat gcgttactcc ttataatatt
     4441 taatacgcgg ttattgtacc tctttgtatt taatgagaac aaagccccaa cattaattga
     4501 aaattcgcct ctttcgaggc ttctccgcag cttgacgctg ctacaatttt caatttatgt
     4561 tgggatgtcc aataaccaca tacgcccttg catgcgagca tgcagatgcg catgcggtta
     4621 ttgtacctct ttgttcacta acaaagggtg cacttcgccg gaagtacacc ctaaatctta
     4681 aaatgttctt cctcctcata cgtgaagcaa ctatcatctg ctcacagtat ttggtgtcag
     4741 tgactctgaa ttataacatc agagccttca atgtctgcag gcttgaagaa agagagctgt
     4801 tatcagcatg tctcgcttct tgcgtgctat ctaatatcta atccgattgt atcatgtcta
     4861 aatcatttca acaatattat atctgcttgt ttcccgaaat atacactatt ttgggacaaa
     4921 aaaagaacag ccatcgttct gctgtccttt aggagaaggt attttactat ggggatagta
     4981 aaaaatatat aatgtattgg gggggtttta cgttaattat agtatcacgt gccatgaaaa
     5041 atgtctatta tcgtgatgca caaacatgca taaattttcg attatttttt atgcaatatg
     5101 tatatgattg atttaagata atcatttggt tacaattacg gcccattaag aatttcaata
     5161 tttttgaaaa gaaatctact tacaagttga cttttttaac ctaaaggaag aaaatagaaa
     5221 cataaattgg aaggaaagga gcggactctc atgaagaaaa tactggtttt accactgttg
     5281 ctttgtctta tattctgctc ctttctaagt ctcgacaaaa atctggacga atttgatcct
     5341 gaccgcacag catacggttc atacgatcac agggttgtct ggggctctta tgcctcttct
     5401 gataaggccg cgcttccaca gcaggaagtc ttggactcac gcgcagtgct ttattcagag
     5461 tcagatatgt cggcactcat ggattgccag tatatagaag aaataccttc ttttcctgaa
     5521 gtcatatgcc gcatccgtac ctcttcgctt ggttttttag gcaggcttac atccaattta
     5581 agctggctgg gtgcgttact ccagttaata catattattt tattcgcata tgccgctatc
     5641 gtggtcatgc gcatatgcct cacaaacacg agccggcgct ttattatccg ttttatccac
     5701 gacaaggatg ggcataaact aatttccttc gctgattatt tcaaaactcg cgcatatgcg
     5761 gaggaaatat aacttagata cattaaataa acattttctc cgcgtcatgc tgacaaagta
     5821 aaacggagga tttaaaatgc ttgaagcaat aataattgtc gtgtgtgctg ccgtttgtat
     5881 cggcgccgca gcctatgcct ggtggatgga gaacggtcct gagaaggacg acagtgatgc
     5941 agccggaaag aacagccaag gccatgctta taccaaatac gacagcaata tcaaggatga
     6001 tattgtcttt cacaaagcac acaaataatg ataacattta tcaacattag taactataaa
     6061 cctaaataaa ttatctcacc caattcgtca tgcatcatct ttaacccgga tgatttatgg
     6121 cagaacaaaa cgagccacaa tcctgatacc cacaggattg cggctcgttt ttatatttct
     6181 gtatcttcac ctctgcaaaa cgatgtgcta aatattaagc atatcgctga gctccttaag
     6241 tgctccgtct gtctcatgtg cgagaacctt ctttgcaagc agctttccaa gctgaacacc
     6301 ttcctggtcg aagctgttta cattccataa gaagccctgg aacatgatct tgttctcaaa
     6361 gtgtgcaagg agtgcaccaa gagatgcagg atttacctgg tctccgataa tgatgcttga
     6421 aggacgtcca ccctcgaagt tcttgttctt gttgtcgtct gccttaccac atgcaaatgc
     6481 aactatctga gctgctacgt ttgcacagag
//
LOCUS       DPUF01000174            8398 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6704, whole
            genome shotgun sequence.
ACCESSION   DPUF01000174 DPUF01000000
VERSION     DPUF01000174.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6704"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="DHV81_06225"
     CDS             <1..734
                     /locus_tag="DHV81_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HCI94061.1"
                     /translation="INADKNYIYYVRNNNQKITSQTFFSYDRNSLCRIKRNGHGSTVL
                     DPDPCIYASLIGNYIYYLHYDTQTATSLYRIRIDGEEKKKIKSHYLFTCNTSDRYFYY
                     NNPKNGQLYRYDTASQSEALFYDCNCYKPVVLDDTNVYYMDVNRDNAIVHVNINNPNP
                     VVLTEANIEHYNVYGSLIFYQRGGDNPALCVVKNDGTGFKELAKGEFCNINVTSQYVY
                     FTDFVSNKEYCTSTQNPDTIKALQP"
     gene            complement(791..2092)
                     /locus_tag="DHV81_06230"
     CDS             complement(791..2092)
                     /locus_tag="DHV81_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94062.1"
                     /translation="MRCANCGNELKAGSLYCDVCGHPVQIVPDYNEFDDYLDNLVGND
                     DRRTDGQKTAKPVSDRTIRFDEPVREQLYSAGQWNGQKPDRMSQSVKQQVRQAQTGEQ
                     LSQTKQPCEKPQSDGQRAKRAQQKKIIIISAVVCAVAIVILIAAITRNVSRSHDNSFD
                     YQVKQAKAAYSAGNINSAVSYYEKALSIDSDNTDVRFALADIYMSKKDYDAALVLYQE
                     IINIDPKSKEAYKQLISIYESKKDYDAIVALRESAKDASVLKLFADYTVSKPQFSKSS
                     GKYGETIELSIDADSDTKIYYSYDSDDPLTRGERYYSPITLDKEGTYEITAVAVDDRG
                     IKSEVASAKYEIEFEAPDAPEIDPDGGTFGAQTDITITVPENCKVYYTWDSSDPSAAS
                     TEYTAPIPVPEGNNVLSVIAIDQNTGKCSDIYRSRFEFYMN"
     gene            complement(2129..2968)
                     /locus_tag="DHV81_06235"
     CDS             complement(2129..2968)
                     /locus_tag="DHV81_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCI94063.1"
                     /translation="MANLIDGKLISTQIKDELKEEVAELKKKGIEGCLAVIQVGNDPA
                     SSVYVRNKKKACEYVGIKSLSYELAEETTEDEILKLIEKLNADASVNGILCQLPLPKH
                     IDEDKVIDAIDPKKDVDGFSPQSVGAMVIGKPGFLPCTPAGIIQLLKRSNIDIDGKSC
                     VVVGRSNIVGKPMSLLMLRENATVTVCHSHTKDLKDVCKNADILIVAIGKPKFIDASY
                     VKDGAVVIDVGIHRNAENKLCGDVDFDSVVSKASHITPVPGGVGPMTIAMLMSNCVEA
                     MKR"
     gene            complement(2977..4647)
                     /locus_tag="DHV81_06240"
     CDS             complement(2977..4647)
                     /locus_tag="DHV81_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCI94064.1"
                     /translation="MKTDIQIAQEATMLPIKDVAASIGIEEDDLELYGKYKAKISDEL
                     INRTKKNPDGKLILVTAINPTPAGEGKTTTSVGLGEAFGRLGKKALIALREPSLGPCF
                     GIKGGAAGGGYAQVVPMEDLNLHFTGDFHAITSANNLLAALLDNHIQQGNELGIDPRQ
                     IVWKRCMDMNDRVLRNIVVGLGSKMDGMVREDHFVITVASEIMAILCLADDMADLKKR
                     LGRIIVAYTFDGKPVTADDLQATGSMAALLKDALKPNLIQTLEHTPAIVHGGPFANIA
                     HGCNSVRATKTALKLADYVITEAGFGADLGAEKFFDIKCRMAGLKPDAVVLVATIRAL
                     KYNGGVPKDELSSENLDALKAGIVNLEKHIENLHKFGVPVVVTLNSFVTDTKAETDFV
                     EQFCKERGCEFALSEVWEKGGEGGIDLANKVLETIEHKESNFKVLYDDSLSLKEKIET
                     VAKEIYGADGVTYSPAAERELKRITDLGMGDFPVCMAKTQYSLSDDAKKLGRPSGFKI
                     NVREVYASAGAGFVVAVNGSIMTMPGLSKKPAAYGIDVDDNGVITGLF"
     gene            complement(4667..7498)
                     /locus_tag="DHV81_06245"
     CDS             complement(4667..7498)
                     /locus_tag="DHV81_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCI94065.1"
                     /translation="MATRKKSSAKRQTKKERMAQIERQQAFKKEIFLWIVVAVSILLF
                     ISNFGIGGNLGNAVSGFLFGLFGMVAYIFPLVLLVGSFFAVSNKGNSYAIMKLVMTIV
                     LIWFICVFMYLAVYGEFAVSPVQSYIDSVERHSGGGFIGALIGCILVPAVGIIGAYVV
                     TIVVMIICLVLITGKSFMKGMRNGGRKVYESARENNERYQEYRQYKSRERQTRDGLAS
                     DDTESDIKEAAKGGRRSKRAGRRMDNKITGVSLDTKIDGSENALDSGMAGSDTAHVGI
                     FGQGAAATDALGEITIENYPSSPAIKEKKTTSLTSGDAGEIVQTPDFGVASEPVSVLG
                     GTADSDSADELGRKTLQQINGMKPDSSGAAAASHVSQIPISGCVEPDISDDFGIQKDN
                     ELQSKLSAPVADVVMTKPPAAEPGKKKVKSDKDELAKEVEKVSEQIAINDAENAIQQK
                     PYVFPDVDLLKAPDRGKTGDSQAHLRETAAKLEQTLNVFGVNAKVNNISCGPAVTRFE
                     ITPELGVKVSKIVNLADDIKLNLAAADIRIEAPIPGKAAVGIEVPNSQSVAVSFRELV
                     ESEEFKNAKSKITFAVGKDIAGKVKVTDIAKMPHLLIAGATGSGKSVCINTIIMSILY
                     KAKPDEVKLIMIDPKVVELSVYNGIPHLMIPVVTDPKKAAGALNWAVSEMTDRYEKFA
                     NSGVREINGYNAMIDAMDGKDTEKPPKMPQIVIIVDELADLMMVASKDVEEAICRLAQ
                     LARAAGIHLIIATQRPSVNVITGLIKANMPSRIAFAVTSGVDSRTILDMNGAEKLLGK
                     GDMLFDPQGVPKPLRVQGAFVSDKEVSDIVKFIIENNENAQYSNDVAQKMESLSNDTT
                     NTTVTISDVENTDDGRDSYFAEAASIITDKERASIGMLQRYLKIGFNRAARIMDQLEE
                     AGVVGPEEGTKPRKVLVTKEELDTILENN"
     gene            complement(7844..>8398)
                     /locus_tag="DHV81_06250"
     CDS             complement(7844..>8398)
                     /locus_tag="DHV81_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HCI94066.1"
                     /translation="MIGLISRLTDQKGLDLINHVIEGIVDDFTQLVVIGTGDPQYENM
                     FRHYAWKYPDRVSANICYSDDLAHKLYAAADAFLMPSRFEPCGLTQLISFRYGTVPIV
                     RETGGLKDTVKPYNEYENSGDGFSFSNYNADEMLAVINYSKHIFFDKKREWNQIVDRG
                     MANDFSWNASKFKYEGLYNYLIGE"
BASE COUNT         2267 a         2132 c         1480 g         2519 t
ORIGIN      
        1 acataaacgc agataaaaac tatatctatt acgtgagaaa taacaatcag aaaatcacaa
       61 gtcagacctt cttttcatat gacaggaaca gtctctgccg cataaaaaga aatggccacg
      121 gctccaccgt gctggatcct gacccatgca tctatgcatc cctgataggc aactacatat
      181 attatctgca ctatgacaca caaacagcta cctctctata ccgcatcaga atagatggag
      241 aagaaaaaaa gaaaataaaa agtcactatc tgttcacctg caacacatca gaccggtatt
      301 tttattataa taatccaaaa aacggacagc tatacagata tgacactgct tcacagagtg
      361 aagccttatt ctacgactgc aactgctaca agccggttgt gctggatgac acaaatgtgt
      421 actacatgga tgtaaatcgt gacaatgcaa ttgtccatgt caatatcaac aatccaaatc
      481 ctgtggtttt aacggaagcc aatattgagc attacaatgt atatggcagt ctgatattct
      541 atcagcgcgg cggtgataat ccggcactgt gtgttgtaaa aaatgacggc accggcttta
      601 aagagcttgc taagggtgag ttttgcaaca taaacgtaac atcacagtat gtgtatttca
      661 cagattttgt ctcgaacaaa gagtactgta catccacaca gaatccggat acaataaaag
      721 ctttacagcc ataacagata atttcattaa aaagaacacg catgattaat caactcatgc
      781 gtgtttttta ttaattcata taaaattcaa atcttgatct gtatatatca ctgcactttc
      841 cggtattctg gtctatcgca ataacagaaa gcacattatt tccctcagga acaggtattg
      901 gagctgtata ctctgttgat gcagcactcg gatctgacga atcccaggta tagtatacct
      961 tgcaattttc aggtactgtg attgtaatgt ctgtctgtgc accaaatgta cctccgtcag
     1021 ggtctatctc cggtgcatcc ggtgcctcaa actcgatttc atacttggca gatgccacct
     1081 cgcttttgat tcctctgtca tcaaccgcaa cagctgttat ctcatatgtg ccttccttgt
     1141 ccagggtgat cgggctataa tatctttctc ctctggtcaa aggatcatcc gaatcatatg
     1201 aatagtatat tttggtatcg ctgtctgcat ctatagaaag ctcaatagtc tctccatact
     1261 tgccactgct cttgctaaac tgcggtttag atacagtata atccgcaaaa agctttaaaa
     1321 cagatgcatc cttagcactc tcacgcagcg caacaattgc atcataatcc ttttttgatt
     1381 cataaatcga gataagctgc ttataagcct ctttgctctt tggatcaata ttgataattt
     1441 cctgatacag cacaagagcg gcatcataat ccttcttcga catatatata tcagccaaag
     1501 caaatctgac atctgtatta tccgaatcaa tcgacaaagc cttctcataa taagaaaccg
     1561 cggaatttat attccccgcc gaataagccg cctttgcctg cttcacctga taatcaaatg
     1621 aattgtcatg tgatctgctt acattacgtg taatagctgc aataagtatt acaattgcta
     1681 cagcgcacac tacagctgat ataataatta tcttcttctg ctgcgctctc tttgccctct
     1741 gtccgtcaga ctgcggcttc tcacacggtt gtttcgtctg agacagctgc tcacctgtct
     1801 gagcctgtct cacttgctgc tttacagact gtgacattct gtccggtttt tgtccgttcc
     1861 actgaccggc actataaagc tgttccctca ccggctcatc aaacctgatt gtcctatcag
     1921 acacaggctt tgcagtcttt tgtccatcag ttcgcctgtc atcatttcca accagattat
     1981 caagataatc atcaaattcg ttgtaatcag gcactatctg aaccggatgc ccacagacat
     2041 cacagtacag actgcccgcc ttcaattcat taccacaatt ggcacatctc atacatctaa
     2101 tcctcttact tattgtctaa ttgtctaatt atctcttcat agcctcaaca caattgctca
     2161 tgagcatcgc aattgtcata ggacctacac cgcccggtac aggtgtgata tgtgacgctt
     2221 tagacacaac agaatcaaaa tccacatcac cacacagctt attttcagca tttctgtgga
     2281 ttccaacatc aatgacaacc gcaccatcct tcacatatga agcatctata aacttaggct
     2341 ttcctattgc cactataagt atatcagcat ttttacatac atcttttaaa tcctttgtat
     2401 gtgaatgaca taccgtaaca gttgcattct ccctaagcat gagaagtgac ataggcttgc
     2461 cgactatatt gcttctgcca accacaacac agcttttacc atcaatatct atattgcttc
     2521 tctttaaaag ctgaatgata ccggccggag tacacggcaa aaaaccaggc tttcctataa
     2581 ccatcgctcc aacactctgt ggactgaagc cgtcaacatc cttcttcggg tcaatagcat
     2641 caataacctt gtcctcatcg atatgctttg gcagtggaag ctgacagaga ataccattta
     2701 cagaagcatc agcattgagc ttttcgataa gttttaatat ctcatcctcc gtagtctcct
     2761 ctgcaagttc atacgaaagc gatttgattc cgacatactc acatgccttt ttcttgttgc
     2821 gcacatacac tgatgatgca gggtcatttc cgacctgaat caccgcaagg cacccctcga
     2881 tacccttttt cttaagctca gccacttctt ccttaagctc atccttaatc tgcgttgaaa
     2941 ttaattttcc gtctattaga ttagccatat ttcctcctag aataatcctg tgatcacacc
     3001 attgtcatcc acatcaatgc cgtatgcagc cggcttcttt gaaagtcccg gcatagtcat
     3061 gatagaaccg tttactgcta ccacaaagcc tgcacctgct gacgcataaa cctcacgtac
     3121 gttgatttta aagccgctcg gtctgccaag cttcttagca tcatcagaaa gcgaatactg
     3181 tgtctttgcc atacatacag ggaaatctcc cattccaagg tctgtaattc tcttaagttc
     3241 tctctctgct gccggagaat atgtaacacc atctgctcca tatatttctt ttgcaacagt
     3301 ttcaatcttt tccttaagtg acaggctatc atcatagagc accttaaaat tgctttcctt
     3361 gtgctcgatt gtttcaagta ccttgttggc aaggtcaatt ccgccttcgc cacccttctc
     3421 ccatacctct gagagtgcaa attcacagcc tctttcttta cagaactgct ctacaaaatc
     3481 agtctcagcc tttgtatctg tgacaaatga atttaatgtg acaactacag gcactccaaa
     3541 cttgtgaaga ttctcaatat gcttctcaag atttacgatt ccagccttaa gtgcatcaag
     3601 attctctgat gaaagctcat cctttggcac accgccatta tattttaatg ctctgattgt
     3661 tgcaacaagt acaactgcat ccggcttaag ccctgccata cggcacttga tatcaaagaa
     3721 cttctctgcg ccaaggtctg cgccaaagcc tgcctcagtg ataacatagt ctgcaagctt
     3781 taatgctgtc tttgtggcac ggacactgtt acagccatgt gctatattgg caaaaggtcc
     3841 accgtgaact attgccggtg tatgctcaag tgtctggata aggttcggct ttaaggcatc
     3901 ctttaagaga gctgccatag agcctgtagc ctgaagatca tcggctgtga caggctttcc
     3961 gtcaaaggtg tatgccacga taattctgcc aaggcgcttc tttaaatctg ccatatcatc
     4021 ggccagacag agtattgcca taatttcaga tgctacagtg atgacaaaat gatcttctct
     4081 gaccattccg tccatcttgc ttccaagacc aacaacgata tttctaagga cacggtcatt
     4141 catatccata catctcttcc atacaatctg gcgtgggtcg ataccaagct cgtttccctg
     4201 ctggatatga ttgtcaagaa gtgccgcaag cagattgttc gcagatgtaa ttgcatgaaa
     4261 atctcctgta aaatgaagat ttaaatcctc cattgggaca acctgtgcat atccgccacc
     4321 ggctgctcct cccttgatac caaagcaagg tccaagtgac ggctcacgaa gtgcgataag
     4381 tgctttcttt cccaggcgtc caaatgcctc tccaagacct acgcttgtgg ttgtctttcc
     4441 ctctcctgca ggagtcgggt ttatagctgt aacaagtatg agctttccgt caggattttt
     4501 ctttgtgcga ttgataagct catcagaaat ctttgcctta tactttccat aaagctctaa
     4561 gtcatcctct tcaatcccta tagatgctgc tacatccttg attggaagca ttgtagcttc
     4621 ctgtgcgatc tgaatatctg ttttcatttt tctttgctcc tttgtgttaa ttattctcta
     4681 atatagtatc taattcctct ttagtcacca gaaccttgcg gggctttgtc ccctcctcag
     4741 gtcccactac accggcctct tcgagctgat ccatgatgcg ggccgccctg ttgaagccaa
     4801 ttttaaggta tctttgaagc attccaattg aagcccgttc cttatctgtg ataattgacg
     4861 ctgcctccgc aaagtaagag tccctgccat catccgtgtt ttccacatct gaaatggtaa
     4921 cggttgtatt tgtcgtatca ttcgacaggc tctccatctt ttgtgccaca tcattactgt
     4981 actgtgcatt ctcattattt tcaataataa atttcacaat atcggaaact tctttatctg
     5041 aaacgaatgc gccctgtact cgaaggggct tcggaacacc ctgtgggtca aataacatat
     5101 ctccctttcc aaggagcttt tccgcaccat tcatatcaag tatggtacga ctgtcaacac
     5161 cacttgtaac cgcaaatgcg atacggcttg gcatattcgc cttgatgaga cctgtaataa
     5221 cattaacaga aggcctctgt gtagctataa tcagatgtat accggctgct ctcgcaagct
     5281 gtgccagcct gcatattgcc tcctctacat cctttgatgc aaccatcata agatctgcaa
     5341 gctcgtccac tataattaca atctgtggca tctttggcgg tttttctgta tccttgccat
     5401 ccattgcatc aatcatggca ttgtaaccgt ttatttccct gacaccgctg tttgcaaact
     5461 tctcgtatcg gtctgtcatc tctgaaaccg cccagtttaa tgctcctgca gcttttttag
     5521 gatctgtaac aaccggaatc atcagatgag gtatgccatt gtatacacta agctcaacca
     5581 ccttcgggtc aatcataatc agctttacct catccggctt tgccttgtat agtatactca
     5641 tgataattgt attgatacaa actgacttac ctgagccggt agcacctgca attaaaagat
     5701 gtggcatttt tgcgatatca gtaaccttaa ccttgcctgc aatatctttt cccactgcaa
     5761 aggtaatctt tgatttggca tttttaaatt cttcagactc cacaagctcc ctgaaggaaa
     5821 cagcaacact ctggctgttt ggcacttcga tgcccacagc ggcttttccc ggaataggag
     5881 cctcgattcg tatatccgca gctgccagat taagctttat atcatctgcc agattcacaa
     5941 tctttgatac cttcactcca agctccggtg tgatttcaaa ccttgtaaca gcaggaccgc
     6001 agcttatatt attcaccttc gcattcaccc caaacacatt aagagtctgt tcaagctttg
     6061 ccgccgtctc acgcagatga gcctgcgaat caccggtctt ccccctgtca ggagccttaa
     6121 gcagatcaac atccggaaat acatacggct tctgctggat tgcattttct gcgtcattta
     6181 tcgctatctg ctcgctgact ttttcgacct ctttggcgag ctcatcctta tctgatttga
     6241 cttttttctt acctggctct gctgcaggtg gttttgtcat taccacatct gcaaccggag
     6301 ctgataattt tgattgcagc tcattatctt tttgtatgcc aaaatcatca gatatatcag
     6361 gctccacgca gcccgatata ggtatctgcg acacatggct tgcagctgct gcgcccgagc
     6421 tgtccggttt cattccatta atctgctgca gggtctttct gccaagctca tctgcactat
     6481 cactatcggc tgtgccaccc aaaaccgaaa ccggctccga tgccacacca aagtccggtg
     6541 tttgcactat ttcccctgca tcccctgatg taaggcttgt cgtctttttc tcctttattg
     6601 caggtgaact cgggtaattc tctatagtta tctcaccaag cgcatctgta gctgctgcac
     6661 cttgtccaaa tatccccaca tgcgctgtat cactacctgc catgcctgaa tccagcgcat
     6721 tttcactgcc atcaattttt gtatcaagtg acacgcctgt tatcttattg tccatccttc
     6781 ggccggcacg cttactcctt ctgccacctt ttgcagcctc ctttatgtca ctctctgtat
     6841 catctgaagc taaaccatct cttgtctgac gctctcttga cttatactgc cgatactcct
     6901 gatatctctc attattctca cgcgctgact cgtatacctt tcggccgccg tttctcatac
     6961 ccttcatgaa tgacttaccg gtaatgagca caagacagat aatcatcaca actattgtca
     7021 ccacgtatgc acctataatt cctaccgccg gaactaaaat gcagcctata agagcaccta
     7081 taaagccacc acctgaatgc ctttcaacac tgtcaatgta tgactgcacc ggcgaaaccg
     7141 cgaattcacc atacactgcc aggtacataa acacacatat aaaccatatg agaactatgg
     7201 tcataacaag cttcatgata gcatatgaat tgcccttatt ggacacagca aaaaagctgc
     7261 ccaccagaag cacaagtgga aaaatatatg ccaccattcc aaataaacca aaaaggaaac
     7321 cgcttactgc gtttcccaaa ttaccgccta ttccaaaatt gcttataaat aaaaggattg
     7381 aaacagctac tactatccag agaaatattt cctttttaaa ggcctgctgc ctctctatct
     7441 gagccattct ctcctttttt gtctgtcttt ttgctgagga tttcttcctc gttgccatgt
     7501 aactaacctc cgtattttga tttacagagt atataataac atatttcttc tgttctgtgt
     7561 aaattttcta aagtaatttt tcgattttgt gattaaaggg tcaaaaggta tcatcgatgc
     7621 atgcttgcat gcaattcgat gtatacctta ttgatccgca ctacaccgag aaagtagcaa
     7681 tttgctgcgg ggacgtagca aattagcgga tttcgaggtg gagtcagatt acgggaatgc
     7741 gtagcatgta gtaatctgat taatcataaa tgaggtgcaa aataccccgc catgaatggc
     7801 gggggcaaaa tgttatgcaa aaagcacaat tttacaaaat attttattca ccaataaggt
     7861 aattgtataa tccctcatat ttgaatttcg aagcattcca agagaaatca ttggccatgc
     7921 ccctgtccac aatctggttc cactcacgct tcttatcgaa gaagatgtgc ttagaataat
     7981 tgatgactgc aagcatctca tcggcattgt agtttgagaa tgagaagcca tctcctgaat
     8041 tctcatactc attgtaaggc ttaaccgtat ccttgagacc tcctgtctca cgaacaatcg
     8101 gaactgtacc atagcggaat gaaataagct gtgtcagacc gcaaggctca aacctggacg
     8161 gcatcaaaaa tgcatcagca gccgcataga gcttgtgagc cagatcatcg gagtaacaga
     8221 tatttgccga aactctgtca gggtatttcc atgcatagtg tctgaacata ttctcgtact
     8281 gaggatctcc tgtaccgata acaacaagct gtgtgaaatc gtccacgata ccttcaatca
     8341 catgattgat aaggtcaaga cctttctggt ctgtaagtct tgaaataaga cctatcat
//
LOCUS       DPUF01000175            2634 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_30969, whole
            genome shotgun sequence.
ACCESSION   DPUF01000175 DPUF01000000
VERSION     DPUF01000175.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_30969"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="DHV81_06255"
     CDS             <1..753
                     /locus_tag="DHV81_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCI94067.1"
                     /translation="ETFVACDSEKEAKAFSDTYISEEKNKLIEDTKAKMSLDDLFSQI
                     QSGNMKELDIIVKADVQGSVEAVKQSLVKLSNEEVVVKVIHGGVGAINESDVILASAS
                     NAIIIGFNVRPDPVAKATADREGVDIRLYKVIYNAIEDVSAAMKGMLDPVFEEKVIGH
                     AEIRQIFKASGVGNIAGSYVLDGFMERGCRVRISREGEQIFEGDLASLKRFKDDVKEV
                     KTGYECGLVFDGFNDIQELDIVEAYKMVEVPR"
     gene            829..1194
                     /locus_tag="DHV81_06260"
     CDS             829..1194
                     /locus_tag="DHV81_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCI94068.1"
                     /translation="MRKNSIKNIRINEEVMRELSNIIRGEIKDPRINPMTSVVAVEVA
                     PDLKTAKAYISVLGDKKSQADTLAGLKSAEGYIRRTLAKNINLRNTPAITFIIDQSIE
                     YGVEMSKKIDEVTKNLKED"
     gene            1209..>1517
                     /locus_tag="DHV81_06265"
     CDS             1209..>1517
                     /locus_tag="DHV81_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCI94069.1"
                     /translation="MTEIDSILKDAQSVAVSGHVRPDGDCVGSTLAVYNYIKDYYPDI
                     NARLYLEPSPNIFKFMQRADEIDSSYADDTVYDLFIACDCSDTGRLGNAAKYFESAKR
                     T"
     assembly_gap    1518..1627
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1628..2164
                     /locus_tag="DHV81_06270"
     CDS             <1628..2164
                     /locus_tag="DHV81_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCI94070.1"
                     /translation="KERITIQIAECIYTGMVHDTGVFQYSCTSKKTMDIAGSLMELGI
                     DYSRIIDDTFYTKTFAQNKIMGQALLNAALYLNGDVILSAVSRAEMEKYEVLPKHLDG
                     IVNQLRVTKDIKVAVFLYENEDGTYKGSLRVNGDYDVAKVASVFGGGGHVKAAGFTIE
                     GPLEVATDRILTAIKDNI"
     gene            2183..>2634
                     /gene="truB"
                     /locus_tag="DHV81_06275"
     CDS             2183..>2634
                     /gene="truB"
                     /locus_tag="DHV81_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCI94071.1"
                     /translation="MVNGIINVYKEKGYTSFDVVAKLRGIFHQKKIGHTGTLDPDAQG
                     VLPVCLGKATRVCDLLTDKDKVYKAVLLLGQETDTQDISGQILNQAKVNVTEQAVYDA
                     ISQFIGRQKQVPPMYSALKVNGKKLYELAREGRVIERKARDIEVFDIKV"
BASE COUNT          786 a          430 c          663 g          645 t
ORIGIN      
        1 gagacatttg tggcatgtga ttcagagaag gaggcaaagg cattctctga cacatatatt
       61 tcagaggaga agaataagct catcgaggat acaaaggcaa agatgtcact tgatgatctg
      121 ttctcacaga ttcagtccgg caatatgaag gagcttgata tcatcgtaaa ggcagatgta
      181 cagggttcag ttgaggctgt taagcagagt cttgtcaagc tttcaaatga ggaggttgtg
      241 gtcaaagtca tccatggcgg cgttggtgcc atcaacgagt cagatgttat ccttgcatca
      301 gcatcaaatg caatcatcat tggctttaac gtcagaccgg atcctgttgc caaggctaca
      361 gcagaccgtg agggcgtaga tatcagactt tacaaggtta tctacaatgc aatcgaggac
      421 gtatccgctg ccatgaaggg tatgcttgat cctgtatttg aggagaaggt tatcggacat
      481 gcggaaatca gacagatttt caaggcttca ggcgttggaa atattgcagg ttcatatgtg
      541 cttgacggat ttatggagag aggctgccgt gtcagaataa gcagagaggg tgagcagata
      601 ttcgagggag accttgcttc actcaagaga ttcaaggatg acgtcaagga ggtcaagaca
      661 ggctacgagt gtggtcttgt atttgacgga tttaatgata ttcaggaatt ggatatagta
      721 gaagcctaca agatggtaga ggttccaaga taatcatata ttggttatct tgaattaatg
      781 cggctattcg gatagttgcg gtttaatata aaaagaaagc gggattatat gagaaagaac
      841 agcataaaaa atattcgtat caacgaagag gtaatgcgtg aactgtcaaa tatcatacgc
      901 ggtgagataa aggatccgcg tatcaatccc atgacaagtg tagttgctgt cgaggtggca
      961 cctgatttaa agacagcaaa ggcttatatc agtgtgcttg gtgacaagaa atcacaggct
     1021 gatacacttg caggacttaa gtcagcggag ggatatatac gcagaactct cgcaaagaac
     1081 atcaatttga gaaacacacc ggctattaca tttatcatag accagtctat agagtatggt
     1141 gttgagatgt ctaaaaagat tgatgaagta acaaaaaatc tcaaagagga ttaacaagag
     1201 gattgattat gacagagatt gattctattt taaaggatgc acaatcagtt gccgtttccg
     1261 gtcatgtgag accggatggc gactgtgtcg gctcaacgct tgcggtatac aactacataa
     1321 aggattatta tcctgatata aatgcaaggc tttatttaga gccaagcccg aacattttta
     1381 agtttatgca aagagcagat gagattgaca gcagctatgc agatgatacc gtatacgatc
     1441 tgttcatagc atgtgactgc agtgacacag gcagactggg aaatgcggct aagtattttg
     1501 agtctgcaaa aaggacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnaag gagcgcatca caatacagat agcagagtgt atttacacag gcatggtgca
     1681 tgatacaggc gtgttccagt attcatgcac ttcaaaaaag actatggata tagcaggctc
     1741 ccttatggaa ctgggtattg attattcaag aataatcgat gatacatttt atacaaagac
     1801 atttgctcag aacaagatta tggggcaggc gcttttgaat gctgctttgt atctgaatgg
     1861 tgatgttata ttgtctgcag tatcgcgcgc tgaaatggaa aagtacgagg tacttcctaa
     1921 gcatctcgac ggcattgtaa atcagctgcg cgtgacaaag gatataaagg ttgctgtatt
     1981 tctctacgaa aatgaggatg gtacctacaa gggcagcctg cgcgtaaacg gtgattacga
     2041 tgtggcaaag gttgcgtcgg tatttggcgg cggtggacat gtaaaggccg ccggatttac
     2101 aattgaagga ccgcttgagg tggcaacaga ccgcatcctt acggcaatta aggataatat
     2161 ttagggagag attcttgggc agatggttaa tggaataatc aatgtataca aggaaaaggg
     2221 ctatacaagc tttgacgttg tggcaaagct tcgaggcata ttccaccaga agaagattgg
     2281 tcatacaggt acacttgatc ctgatgcgca aggcgtcctt cctgtctgcc ttggaaaggc
     2341 aaccagagtg tgtgaccttt tgacagataa ggacaaggtc tataaagcgg tgctgctttt
     2401 aggacaggag acagatacac aggatatttc agggcagatc ttaaatcagg ctaaggtcaa
     2461 tgtgactgag caggcggtgt atgatgcaat atcacagttt attggcagac aaaagcaggt
     2521 gccgccaatg tattcggctc taaaggtcaa tggaaaaaag ctctatgagc ttgcaagaga
     2581 gggcagggtg atagagagaa aagccaggga catcgaggtg tttgatatca aggt
//
LOCUS       DPUF01000176            2584 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_39638, whole
            genome shotgun sequence.
ACCESSION   DPUF01000176 DPUF01000000
VERSION     DPUF01000176.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_39638"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="DHV81_06280"
     CDS             <1..948
                     /locus_tag="DHV81_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase"
                     /protein_id="HCI94072.1"
                     /translation="DYYQSLREDKSNPLWNYATQEQTAPGSTFKMVSSTAGLAEGVID
                     TSSKINCTGIFTEISNQPKCWIYPGAHGMDNVSEALRDSCNVFFYTTGFRLASKDTGS
                     YDDENGMKYIQKYASIYGLDQKSGVEIVEKTPSIATQYPVMAAIGQSNNNYTTISLSR
                     YVTAVASGKLYDYKLMNKIVDADGKTVKQYDSKSTDISGTLTQSQWDAIHQGMRMVVE
                     DLHDVFGGFTGVEVSGKTGTAQQVETRPNHALFVGYAPSSDPEITIATRISSGYSSHN
                     AAAASRNIISYYYNLESLDNLLAVKAEGVNSSGSNARTD"
     gene            932..1708
                     /gene="minD"
                     /locus_tag="DHV81_06285"
     CDS             932..1708
                     /gene="minD"
                     /locus_tag="DHV81_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCI94073.1"
                     /translation="MPEQTRRFIFVSEAIVFTSGKGGVGKTTVISNIGVELSQLDKKV
                     IMLDTDMGLRNLDLVMGIEDKVNYNILDILNRSCRIRQAIIRNKKYPNLYVIPAAPSM
                     DTLCSYEARFKILIEELKASFDYCLIDSPAGIDSGFWFSVSPADRAIVVTTPHVSAIH
                     DARRCISLLDSAHLDDISVIVNAYDKHMVRRHQMISDNDITALLSTRIIGTIPYDKSV
                     IICQNRGIPVREAKSRLAPVFAHISGQIIHSSDDMRGAAV"
     gene            1705..1917
                     /locus_tag="DHV81_06290"
     CDS             1705..1917
                     /locus_tag="DHV81_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94074.1"
                     /translation="MMHKFSKTGEYAKKRLKIALAEDRSTISASDNMTQIKKEIKSVI
                     QRHMNINDEAYEVRIIMRDKKSINNL"
     gene            1992..>2584
                     /locus_tag="DHV81_06295"
     CDS             1992..>2584
                     /locus_tag="DHV81_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCI94075.1"
                     /translation="MLKQYRLKNYHFQLVVYIIALSIIGILLIGSAKHSVQSKQIIGF
                     IMGLTIMIVLSLIDYSFLLKFVWIYYAGMNVLLLLVLFAGDDAKGAQRWFKIGGIRFQ
                     PSEIAKIILILFFAYFFAKYEETINTVKTIVLSVIFVGIPLFLIYKQPDNSTTILTAL
                     IFAALLFISGLSYKIIMPVLGASVTIVLIVISYIYTHA"
BASE COUNT          842 a          515 c          533 g          694 t
ORIGIN      
        1 gattactacc agtctctcag agaggacaag tctaatccgc tatggaatta tgcaacccag
       61 gagcagactg caccgggatc taccttcaag atggtatcaa gcaccgcagg tcttgccgag
      121 ggagtcattg atacttcatc gaagataaat tgtaccggaa tatttacaga aatcagcaat
      181 cagccaaaat gctggattta tccgggtgcg catggtatgg acaatgtatc tgaagcttta
      241 agggattcat gtaacgtatt cttctacaca acaggcttca ggcttgcaag caaggacacc
      301 ggttcatatg acgatgaaaa cggtatgaaa tacatacaaa aatatgcatc tatatacgga
      361 ctggaccaga aatcaggtgt tgagatagta gaaaagacgc cgtccattgc tacacagtat
      421 cctgtaatgg cagctatcgg acagagtaat aacaactata cgactatttc tctttcaagg
      481 tacgtaacag cagttgcttc cggaaagctt tatgactaca agctgatgaa caaaattgtg
      541 gatgcagacg gcaaaacagt caaacaatat gattcaaaat caaccgatat atcaggcaca
      601 cttactcaaa gccagtggga tgccatccat cagggaatgc gtatggtggt cgaagacctg
      661 cacgatgtat ttggcggctt tacaggcgtt gaggtgtccg gtaagacagg aacagcacag
      721 caggttgaga caagaccaaa ccatgcactg tttgtaggat acgctccatc aagcgatcct
      781 gaaattacaa tagctacacg tatatcatct ggatacagct cacacaatgc tgcagctgca
      841 tccagaaata taatttcata ttattataat ctggagtcac ttgacaattt actcgcagtc
      901 aaggctgagg gtgtaaactc atcaggctca aatgccagaa cagactagga ggtttatttt
      961 tgtgagtgaa gcaatagttt tcacttcagg aaaaggcggt gttggaaaaa ctacggttat
     1021 ttccaatatc ggagttgaac tttcacaatt agataaaaaa gtcatcatgc tcgatacaga
     1081 catgggactt cgcaatctag acctggtaat gggaatagag gacaaggtca attacaatat
     1141 cctcgacatc ttaaaccgct cctgccgtat cagacaggca atcatacgca ataaaaagta
     1201 tcccaatcta tatgtaattc ctgcagcacc ttccatggat acgctatgca gctatgaggc
     1261 acgcttcaag atacttatag aggagcttaa agcaagcttt gattattgtc tgatagattc
     1321 tccggcgggt atcgacagcg gcttttggtt ttcagtctca cctgcagacc gtgcgatagt
     1381 agttaccaca ccgcatgtat ctgccataca cgatgcaagg cgatgcataa gcctgctcga
     1441 cagcgcacat cttgacgata tatcagtcat tgttaatgca tatgacaagc atatggtaag
     1501 aagacatcag atgatatccg ataatgatat tacagcactt ctttctacca ggattatcgg
     1561 cactattccc tatgacaaaa gtgttatcat ttgccagaac agaggaatac ctgtccgtga
     1621 ggccaaaagc aggcttgctc ctgtatttgc ccatatatcc ggtcagataa tccattcctc
     1681 tgacgatatg agaggtgccg ctgtatgatg cataaatttt ccaaaacagg agaatacgcc
     1741 aagaaaaggc ttaaaatagc acttgcagaa gataggagca caatatctgc ttctgacaat
     1801 atgacacaga ttaaaaaaga aataaaaagc gtgatacaaa gacatatgaa tataaacgat
     1861 gaagcttacg aagtccggat tatcatgcgc gacaaaaaat caattaataa cctttaacaa
     1921 aatacctttt gaccatcatg tcattagtat tagaattaag attacagaga taacagatag
     1981 gaagtaacaa aatgctcaaa cagtacagac taaagaatta tcactttcaa ttagtagtat
     2041 acataatagc tttgtcgatt ataggcatcc tgctgatagg cagtgccaag cactccgtac
     2101 aaagcaagca gataataggc tttataatgg gactgactat tatgatagtg ctttccctta
     2161 tagattactc atttctgctc aaattcgtgt ggatatacta tgcaggcatg aatgtgctac
     2221 tgttgcttgt tctgtttgca ggtgatgatg ctaagggtgc acagcggtgg tttaagatag
     2281 gaggtatcag attccagccg tcagagattg ccaaaattat actgattttg ttctttgcct
     2341 atttctttgc caaatatgag gaaaccataa acacggtaaa gacgatagta ctgtcagtga
     2401 tatttgtagg aataccatta ttccttattt ataagcagcc tgacaactca acaacaattc
     2461 ttactgcatt aattttcgct gccctcttgt ttattagcgg attaagttat aaaataataa
     2521 tgccggtact tggagcttca gtaacgatag ttttgatagt gataagctat atatatacac
     2581 atgc
//
LOCUS       DPUF01000177            7720 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_38330, whole
            genome shotgun sequence.
ACCESSION   DPUF01000177 DPUF01000000
VERSION     DPUF01000177.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_38330"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="DHV81_06300"
     CDS             <1..1170
                     /locus_tag="DHV81_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCI94076.1"
                     /translation="GLKGVDSSQIEAVRKNIQSIKTQIKDLKSDTELTADKLKLLKES
                     IKADVSSAKSAIDTLKNTFDHSVSPNLTGAVYDIEYALIETQSMLGSLDNSFPDIQKA
                     LDDYSTVLKSGNADIVNTRQYIQQIRDGLQNVISGFDNLSNDEQFNEIVKLLQTDPTL
                     IAQFVTSPLSMEEQKIYEISTYGSAMAPFYTVLALWVGGLITVALVKTKVKDTDDIKS
                     LGKVNSVHKFFGRYITFFVIGQLQTLVTVLGDLFFVGIQCRHPFLFWCASALTSFVFT
                     LLMYSLTAALGNVGQAVAVVIMVIQVAGAGGTFPVEVLPQVYRALYKFMPFNYAMNAL
                     RECVGGMYRFDYCKDLAILLIFIAISLVIGLLLGRLFGKLNTAIEKSKKKSGLIL"
     gene            1332..2363
                     /locus_tag="DHV81_06305"
     CDS             1332..2363
                     /locus_tag="DHV81_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCI94077.1"
                     /translation="MEKKDIDWSNLSFGYQETDYSYVSNYKDGKWDDGQLTKDHTVTL
                     NECAGVFQYAQTCFEGLKAYTTEDGRIVCFRPDLNAQRLKDSCERLEMPVFPEDRFVK
                     AVEEVVKANAAWVPPYGSGATLYIRPYIMGTNAVIGVKPADEYQFRILVTPVGPYFKG
                     GAKPITIRVSDFDRAAPHGTGHIKAGLNYAMSLHAIVDAHAQGFAENMYLDPATRTKV
                     EETGGANFIFITKDGTFVTPKSDSILPSITRRSLMVVAEKYLGLKVEHREVLFDEVKD
                     FAECGLCGTAAVISPVGKIVDHGKEICFPSGMDEMGPVTKKLRDTLTGIQMGHIDGPD
                     GWVHEIKCD"
     gene            2554..3300
                     /locus_tag="DHV81_06310"
     CDS             2554..3300
                     /locus_tag="DHV81_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCI94078.1"
                     /translation="MDEYEKIRQRKREEYRKRHRAPVRRKQLIGNGIVIGMIILIIAT
                     IIIVGALRGKKAKQEEVKAEVTSTLYNPIQPKLDVQLLTPNPYSRPQKALEKVNGIVV
                     HYTANPGTSARQNRDYFNGLAETKKTKASSHFVIGLEGEIVQCIPCNEISYASNNRNS
                     DTISIECCIEDETGKFNDSTYQSLIELTTWLMGRYDLSADDVIRHYDVTGKKCPLYFV
                     EHEDAWEQFHKDLDTYIEENGVPKEEASQN"
     gene            3334..4818
                     /locus_tag="DHV81_06315"
                     /pseudo
     CDS             3334..4818
                     /locus_tag="DHV81_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568146.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
     assembly_gap    4164..4284
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4845..7427
                     /locus_tag="DHV81_06320"
     CDS             4845..7427
                     /locus_tag="DHV81_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCI94079.1"
                     /translation="MSDSKLFQKIQDYFCMVNGIYLSCLTKKDGVMTEVCQCNDKWKS
                     MLTFIGEEKYENLLLRLSDCRVENLIDEPLDKDYIRLYGLIVRVDNTHDIYWIIAAVI
                     DEQMSNDERNLLPDGIIITSEARLNRTIEFLETMTRQLLITKRDEDAANEALSLIRKS
                     DEEIKKQFHMLEAINSVIKLLEMDGSFTDMAQKALESAVTTIKLTGAFIIRKNVDGMH
                     LDVIVSYGRKPFDTISITEVPFFTGKPYIISSDSVMPEKFRLFMEKQAMRAAIFQPVN
                     IDNRTQMYVCFFDEKDDRSWEKYDVKFLNDTKRVIQSILTKKITTNSLAGSYASLEAI
                     LENSGCGIYVADMSKSEILYMNNYCKQLLSNIIEQNKLEKYIFSHTAESRSFTEVYVT
                     EEDKWFDIHRTGIAWVDGRKVQLVTMYDITQKKRYQQRIENQANNDFLTGLYNRMRCE
                     QDLAKFIDDSVKNDTRGAMIYIDLDDFKHINDGLGHQYGDVLLKAISHSLTQVKGIEN
                     HCYRMGGDEFIVIVTGSSVDRLESIINDIQQIFVRPWFLKGEDYYCTISAGVAFFPTD
                     ARTVEDVIRRADIALFNAKKEGKNRIEFYNEGIDGTSTKRLDLEKHMRKATMNECDEF
                     TVYFQPIINVKGEDVCAGAEALVRWNSATMGFINPVDFIPLAEYLGLINPIGEHVLRE
                     AAKHCRYWNDMGHPDYKVNVNLSVVQLLQNDIVKKIKGIIDEVGIEPGNLCLEVTESL
                     AINDMVRMKRILSEIKALGVKVALDDFGTGYSSLNHIREMPLDIIKIDKCFIEHLGED
                     DFSDAFVKMVTELANTIGVHVCVEGVETDRQYAIIKKMGIYYIQGYFFDKPMCIESFE
                     EKYI"
     gene            7472..7684
                     /locus_tag="DHV81_06325"
     CDS             7472..7684
                     /locus_tag="DHV81_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94080.1"
                     /translation="MLFILDIPISAQVVSVADVYDALTSDRVYKRAFSHEKAMQMILD
                     GECGQFNPVLLQCLVNIQDRIKAGLD"
BASE COUNT         2423 a         1339 c         1886 g         1951 t
ORIGIN      
        1 ggcttaaagg gagtggatag ctcgcagata gaggctgtca ggaaaaatat ccaaagcatt
       61 aaaacacaga taaaagacct taagtctgat actgaattga cggcagataa gctaaagctt
      121 cttaaggagt cgataaaagc cgatgtgtca agtgccaagt ctgcaataga tacacttaaa
      181 aatacatttg accacagcgt gtcaccgaat ctcacaggag cggtatatga tatagaatat
      241 gcacttattg agacacagtc catgcttggt tctttggaca acagcttccc ggatatacaa
      301 aaagcactag atgattattc aacagtactt aaatcaggaa atgcagatat cgtaaatacc
      361 agacagtata tacagcagat cagagatggt ttacagaatg ttatatcagg atttgataat
      421 ttatcaaacg atgagcagtt taatgagatt gtaaagctgc ttcagacaga tccgacactc
      481 attgcgcagt ttgtgacatc accgttgtct atggaagagc agaagatata tgagatatcc
      541 acatatggct cagccatggc accgttttac acagtgctgg ctctgtgggt gggaggtctg
      601 ataactgttg cacttgtgaa aacaaaggta aaggacactg acgatataaa atcacttggc
      661 aaagtaaaca gcgtacataa gttttttggc agatatatca catttttcgt gataggacag
      721 ctgcagacac ttgtgacagt gctcggtgat ttgttctttg tcggaataca gtgcaggcat
      781 ccgttccttt tctggtgtgc aagcgcactg acaagctttg tattcacgct tcttatgtac
      841 tcactcacag cagccttggg caatgtggga caggctgtag cagtagtcat tatggtcatt
      901 caggtggcag gcgcaggtgg cacattcccg gttgaggtgc tgccacaggt gtacagagca
      961 ctgtataagt tcatgccgtt taactatgcc atgaatgccc taagagagtg cgtgggaggc
     1021 atgtaccgct ttgattattg caaggatctt gcgattcttc ttatatttat agcaatatcg
     1081 cttgtgatcg gactgctgct tgggcgtctg tttggaaagc ttaatacagc tattgaaaaa
     1141 agtaagaaaa agtcaggcct tatcttataa tagtaaaaaa agctatatag aaacttaagt
     1201 tatggttatt ataaaaaaat agtactagac attttttttc tattgtacta gaatgagcat
     1261 aaatgatagt agacccgata tatgataacg ctaggttata tcggatccgt aaagaaacgg
     1321 aggatataaa gatggaaaag aaggatatcg attggtcaaa ccttagtttt ggctaccagg
     1381 aaacagatta cagctatgtt tctaattata aagatggaaa gtgggacgat ggacagctca
     1441 caaaggatca tacagtgaca cttaatgagt gtgcgggagt attccagtat gcacagacat
     1501 gcttcgaggg acttaaggca tacacaacag aggatggcag aattgtctgc ttcagacctg
     1561 acttaaatgc acagcgtctt aaggattcat gtgagagact tgagatgccg gtattcccgg
     1621 aggatagatt tgtaaaggca gttgaggaag tagtaaaggc aaatgctgca tgggttccac
     1681 catacggctc aggtgctaca ctctatatca gaccatacat tatgggaaca aatgcagtaa
     1741 tcggtgttaa gccggctgat gagtatcagt tcagaatcct tgtaacacct gtaggaccat
     1801 acttcaaggg tggcgcaaag ccaatcacaa tccgtgtatc agactttgat agagcagcac
     1861 ctcacggaac aggacatatc aaggcaggac tcaactatgc aatgagtctt catgccattg
     1921 tagatgcaca tgcacagggc tttgcagaga atatgtacct cgatcctgca acacgtacaa
     1981 aggtagagga gacaggtggt gcaaacttca tctttatcac aaaggatggc acatttgtta
     2041 cacctaaatc agacagcatc ctgccatcaa tcacacgccg ttcactgatg gtagttgcag
     2101 agaagtactt aggactcaag gttgagcaca gagaggtgct ctttgatgag gtaaaggatt
     2161 ttgctgagtg tggactttgc ggaacagcag cagtaatctc accggtagga aagattgtag
     2221 accacggcaa ggaaatctgc ttcccaagcg gtatggatga gatgggacct gtcacaaaga
     2281 agcttcgtga cacacttaca ggcattcaga tgggccatat tgatggaccg gatggatggg
     2341 ttcacgagat taagtgtgat taataataag aatatacatt tataataaag caccgggtat
     2401 tatgttacgt cgtaatatct ggtgcttttg tattatttta tgtggcataa caagacgatt
     2461 tgtgctaata tactcttata tgtatgcaaa tgtttataaa agcttggaat agatatataa
     2521 aagcaaacaa aagagataac cggggataat taaatggacg agtatgaaaa gataagacag
     2581 agaaagcgcg aagaatatag aaagagacac cgcgccccgg ttagaagaaa gcagcttatt
     2641 ggcaatggca ttgttatagg catgataata cttataattg caacgataat cattgtggga
     2701 gctttaaggg gcaaaaaggc aaagcaggaa gaagtaaagg cagaggtgac cagtactctt
     2761 tacaatccta tacagccaaa gcttgatgta cagttgctga cacccaatcc gtattccaga
     2821 ccacagaagg cacttgagaa ggtaaacggc atagttgtcc attatacagc aaatccgggt
     2881 acgtcagcca gacaaaacag agactatttc aatggcctgg cagagaccaa aaagaccaaa
     2941 gcaagctcac actttgttat aggattagag ggtgagatag tgcagtgcat accttgcaat
     3001 gagatatcgt atgcatctaa taacagaaac agtgacacaa tatcgataga gtgctgtata
     3061 gaggatgaga caggaaagtt caacgacagc acataccagt cgctcataga gctcaccaca
     3121 tggcttatgg ggcggtatga tctgagtgct gatgatgtaa taaggcacta cgatgtgaca
     3181 ggcaagaagt gtccgctata tttcgtggag catgaggatg cgtgggagca gtttcacaag
     3241 gatctggata catacatcga agaaaatggt gtgccaaagg aagaggcaag ccaaaactga
     3301 caatatacaa taaattaaag gatgatttaa aaaatgaagg aaattatcgg aaatctgtta
     3361 aaaaaagaga gtgtacgtca gaatctgagc agcctcagac aggagattaa agatgaaaat
     3421 gcattagcag aggctctaaa gctgcttgca ggagaagatg agctgcttgt gtcgtttatg
     3481 ggagcagaag atgcaaaaac cagaaagaac gcagcgcttc tgataggaga tcttcatatg
     3541 tcacagcttt ctgatgaggt ttttaaagca tacgaggctg aacagatgcg ctttgtaaag
     3601 ggctcatatc tggcggcgct gtcacagctg gactgcaaag agctcttgcc acagcttatg
     3661 gaacgtgcaa aagagcttga gcatatgacg gtcacagctg aaaataggaa gcatattgag
     3721 gaagaattaa acgagataaa taaaatcctg attaaatata atgggataaa gcaccataca
     3781 cctgtgctcg agggagtaaa ggctgagctg cttctgatga caaaccgcct tcacagagag
     3841 gtggtacgac gtcagatacc ggttaaggat acaaagctgc atccgcttgg agtgcttgta
     3901 aagactgaca atatacctct tatcatgcag gtgcgcacat tcagaaaaat gtactttacc
     3961 atacatgcag catcgcttct gccaaaggat gcacagaaag cagccggact tcttgcagag
     4021 tcagatatgt atgatatatt aagacgcatg cacctagagg gtggtccgtt ttactatcgt
     4081 attgagagca cggcagatgc ggcgtaccag agcaggcttg cgaaagctat cgatatgcat
     4141 tttgcgggca ggatgatcaa ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnntacaga aagaatgtgc ttcctacgtc gatgcatccg
     4321 tcacaagcgg cacttattgt ttcacttgca aagccgtatc ttaaggaaac ggcacaaatc
     4381 atggatccgt tctgcggagt tggtacactg cttatagagc gcgcacattt agttccggca
     4441 cgcgagatat atgctacaga tacctatgga gatgcaatca ctatgggcag agagaatgct
     4501 gcgtttgcca aaacaagaat taattttata cacagggatt tctttgattt cagacatgac
     4561 tacaaatttg atgagctgat tacggatatg ccggttcgta accgacagac caaggcggag
     4621 atggagcttt tttacgagag gtttttcgac aaggccgcag agcatctggt atccggcgga
     4681 atcatagtca tgtattcaaa tgagattggg tttgtgaaga aacaaatcag gctgcgtgtc
     4741 gaataccgtc tgcttcagga gacatgtatt ctcgataaga atgactttta tttgtttgtt
     4801 atcagatatg aggattgatt tgcgtgagtt tttaggagga aaatatgtca gattccaagc
     4861 tttttcaaaa aatacaggat tatttctgca tggtcaacgg aatatatttg tcgtgtctga
     4921 caaaaaagga tggagtcatg acagaggtgt gtcaatgtaa cgataaatgg aagagtatgc
     4981 ttacctttat tggtgaggaa aaatacgaaa accttctttt gagactgtcg gactgcaggg
     5041 ttgagaatct catagatgaa cctctggata aggactatat caggctgtat ggtctgattg
     5101 taagagtgga caatacccac gatatatatt ggattatagc tgcggtaatt gatgagcaga
     5161 tgtctaatga tgagagaaac ctgcttcctg atggcataat tattaccagt gaagcgcgtc
     5221 ttaacagaac aattgagttc ctggaaacta tgaccagaca gcttttgatc acaaagagag
     5281 atgaggatgc tgcgaatgag gcactttcgc ttatcaggaa gtcagatgag gaaatcaaga
     5341 aacagtttca tatgcttgag gctatcaatt ctgttataaa actgcttgag atggatggaa
     5401 gctttactga tatggctcaa aaagcactgg agagtgcagt tactaccata aagcttaccg
     5461 gtgcttttat catccgcaaa aacgtggacg gcatgcattt ggatgtgatt gtaagctatg
     5521 gcagaaagcc gtttgataca atatcaatta ccgaggtgcc ttttttcacc ggaaagcctt
     5581 acattatatc atcagactct gtgatgccgg agaaattccg ccttttcatg gagaagcagg
     5641 ctatgagggc agctatattc cagcctgtaa atatagataa caggactcag atgtatgtgt
     5701 gcttttttga tgagaaggat gacagaagct gggaaaaata tgatgtgaag tttttaaatg
     5761 atacgaagcg cgtcattcaa agtattctca caaaaaagat aacaaccaac tcgcttgcag
     5821 gctcctatgc ttcgctcgaa gcgatactgg aaaactccgg ctgcggtata tacgtggctg
     5881 atatgtctaa gtcagagata ctttacatga ataattactg taagcagctg ctttcaaata
     5941 ttatcgaaca gaacaagctg gaaaaatata ttttttccca tactgcagag agccggagct
     6001 ttacagaggt ctacgtgacg gaggaggaca agtggtttga tattcatcgc acaggcatag
     6061 cgtgggtgga tggccgcaag gtgcagcttg ttacaatgta tgatatcaca cagaagaagc
     6121 gttatcagca gcgcattgag aaccaggcca ataatgattt cctgacaggg ctatataaca
     6181 ggatgagatg tgagcaggat ctggctaagt ttatagatga ttcagtgaaa aacgacacca
     6241 gaggtgcgat gatttatata gatctggatg atttcaagca tataaatgac gggcttggcc
     6301 atcagtacgg agatgtgctt ttaaaagcta tatcgcatag cctgacacag gtgaagggaa
     6361 ttgagaacca ctgctacagg atgggtggcg atgagtttat cgtcatagtg acaggcagct
     6421 ctgttgacag gctggagagt atcattaatg atatacagca gatatttgtc agaccgtggt
     6481 tcttgaaggg agaggactat tactgtacaa taagtgccgg agtggcattt ttcccgacag
     6541 atgcaagaac tgtggaggat gtcataagaa gagctgatat agctcttttc aatgctaaga
     6601 aagaaggaaa aaaccgtata gagttctaca acgagggtat tgatggcaca agcacaaagc
     6661 gtcttgacct ggaaaagcac atgagaaaag ccacgatgaa tgagtgcgat gagttcaccg
     6721 tgtattttca gccgattata aacgtgaagg gggaggatgt ctgtgcaggt gcagaggctc
     6781 ttgtcagatg gaattcagca accatgggct ttataaatcc ggttgacttt ataccgcttg
     6841 ctgagtatct tgggcttata aacccaatcg gggagcatgt attaagagag gcggcgaagc
     6901 actgcaggta ctggaatgac atgggacatc ctgactacaa ggtcaacgta aatctgtctg
     6961 tggtgcagct gctgcagaat gacatagtta agaaaatcaa aggcataatt gacgaggtgg
     7021 gcatagaacc gggaaatctg tgtcttgagg tgacagagag tctggcaatc aatgacatgg
     7081 tgcgcatgaa gcggatactc agtgagataa aggcgcttgg tgttaaggtt gcactggatg
     7141 attttggtac aggatattca tcactcaacc atatcaggga gatgcctctt gatatcatta
     7201 aaatagataa atgcttcatt gagcatcttg gggaggatga tttttctgat gcatttgtca
     7261 agatggttac agagcttgcc aacacaatag gagtacatgt ctgcgtggag ggagttgaga
     7321 ctgacaggca atatgctatt attaagaaga tgggaatata ttacatacaa gggtatttct
     7381 tcgataagcc aatgtgcata gaaagctttg aggagaaata tatctgacga agaaggtgta
     7441 acatgcacga agaagatata gaaaaaagca catgctattc attttggata taccaatctc
     7501 ggcacaggtg gtatctgtag ctgatgtata tgatgccctg acaagcgatc gtgtgtacaa
     7561 gagagcgttt tcacatgaaa aggcaatgca gatgattctg gatggagagt gcggacagtt
     7621 caatcctgtt ctcctgcagt gtctggtcaa tatacaggat cggataaagg cggggctgga
     7681 ctagcttaaa tgcatagtaa atgaaaaata tatgtaaaaa
//
LOCUS       DPUF01000178            6055 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_15154, whole
            genome shotgun sequence.
ACCESSION   DPUF01000178 DPUF01000000
VERSION     DPUF01000178.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_15154"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="DHV81_06330"
     CDS             complement(<1..752)
                     /locus_tag="DHV81_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94081.1"
                     /translation="MQNPFTTTFSKTPEYTYIHTEKTEEILENFIYDNPSESVYKITG
                     VRGSGKTVILAKVEEELRTNESRYINWLVFDVNPARDILGQIAAMLVKAGFGSQDKKT
                     TGINLSATVMGTGGGIGYTAENNNNFFDIGVEVETMIQAAQKKGKKILIGIDEVSKSE
                     EMIKFASEYGRWLRAGYPVYFVCTGLYENIQELSNVKNLTFFRRAATVKTEPLNMIRM
                     TEMYKSKLDIDSNEAREMAKITKGYAYAFQELG"
     gene            1370..2596
                     /locus_tag="DHV81_06335"
     CDS             1370..2596
                     /locus_tag="DHV81_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCI94082.1"
                     /translation="MTIYDELVARGLIAQVTDEKEIKELINNGKATFYIGFDPTADSL
                     HVGHFMALCLMKRLQMAGNKPIVLIGGGTAQIGDPSGRTDMRQMMTTETINHNVECFK
                     KQMSRFIDFGEGKAIMVNNADWLMDLNYVDVLREVGAHFSVNRMLTAECYKQRMEKGL
                     SFLEFNYMIMQSYDFYTLFQKYGCNMEFGGDDQWSNMLGGTELIRRKLGKDAYAMTIN
                     LLLNSEGKKMGKTQSGAVWLDPNKTTPFEFFQYWRNVSDADVLKCIRMLTFLPLEEID
                     KMESWEGAQLNEAKEILAFELTKLVHGEEEAAKAKEASHALFAGGANNANMPTVTVTA
                     EDFPDGELDIISVLVKAGLCDSRGDGRRNIQQGGVSVADEKVTDISTKYTLDDFKGEG
                     LIIRRGKKKFAKVVAE"
     gene            3130..4134
                     /locus_tag="DHV81_06340"
     CDS             3130..4134
                     /locus_tag="DHV81_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI94083.1"
                     /translation="MKKRSKILASLVLAGTMVLGLAGCGDSGSANGGTQSGKNSDAKY
                     QVGICQLVQHEALDAATKGFKDALKDKLGDDVSFDEQNAAGDASTCSTICTKFVTNDY
                     DLILGNATAALQAASAATDSIPILGTSITDYATALDMSDWSGTTGKNISGTTDLAPLD
                     EQAKCIKELFPDAKNIGIIYCSSEPNSIYQSTTITKYLEDEGYTVKEYTFSDSNDVAA
                     VTQSACDASDAIYIPTDNTAASCTEAINNVASQAKKPIFAGEEGIAKGCGVATLSISY
                     YDLGYTTGEMAYDILVNGTDVKTMEVKSAPKFTKEYLPDRAKELGITVPDGYEEISAE
                     "
     gene            4173..>4525
                     /locus_tag="DHV81_06345"
     CDS             4173..>4525
                     /locus_tag="DHV81_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI94084.1"
                     /translation="MNYFATLNPVAFTNALPGNIAQGLIWGIMALGVYITFRLLDFAD
                     LTVDGSFATGGAVTVMMTINGYNIWVSLLCACIAGLLAGLCTGLLHTKLGIPPILAGI
                     LTQIALYSINLNIMQR"
     assembly_gap    4526..4707
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4708..5157
                     /locus_tag="DHV81_06350"
     CDS             <4708..5157
                     /locus_tag="DHV81_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI94085.1"
                     /translation="GCNPAMSKAQGINIDNMKLIGLALSNAIVALSGGLLSQYSGFSD
                     VNMGRGGIVIGLAAVIIGEVLGDAILGKHMNFMGKLIFVIFGGIVYYIVIGIVLWLQM
                     PSDDLKLFTAIIVAIFLAVPYLKGKSKASFKKAGKRSAAALNAKEGK"
     gene            5158..5952
                     /locus_tag="DHV81_06355"
     CDS             5158..5952
                     /locus_tag="DHV81_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009558576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI94086.1"
                     /translation="MLEIRNVHKTFNPGTINEKHALNGVNLKLEEGDFVTVIGGNGAG
                     KSTMLNAVAGTWPVDDGSILIDGVDVTGLPEFKRASFLGRVFQDPMTGTTATMQIDEN
                     LALAARRGKRRGLGWGITKAEKEQFHELLKELDLGLEDRMTSKVGLLSGGQRQAVTLL
                     MASLQKPKVLLLDEHTAALDPKTAAKVLTLTDKIIKENSLTAMMVTHNMRDAIAHGNR
                     LIMMNNGQVVLNISGEEKKNLTVEDLLMKFEEVSGEEFASDKALLG"
BASE COUNT         1674 a         1340 c         1240 g         1619 t
ORIGIN      
        1 cccaattcct gaaatgcata tgcgtatcct ttcgtgattt tcgccatttc acgagcctca
       61 ttactgtcaa tatccagctt actcttatac atttcagtca ttcgtatcat attaagaggc
      121 tccgttttaa ctgttgccgc tcttctgaaa aatgtaagat ttttgacatt gctcaattcc
      181 tgtatatttt catacaagcc tgtacacaca aaataaaccg gataccctgc tctgagccat
      241 ctgccatatt cagacgcaaa ttttatcatc tcttcggact ttgaaacctc atctattcct
      301 ataagtatct tcttcccctt tttctgtgca gcctgtatca ttgtctcaac ttctacaccg
      361 atatcaaaga aattattatt gttttctgcc gtatagccga ttccaccacc tgttcccatc
      421 actgttgcag agaggtttat ccctgttgtt ttcttatcct gtgaaccaaa accggccttt
      481 acaagcattg ccgctatctg tcccagtata tctcttgcag gatttacatc aaaaacaagc
      541 cagtttatat atctgctttc attggttctt aattcttcct ccacctttgc aagtataacg
      601 gttttaccgg agcctctgac accggttatc ttatataccg actccgacgg attatcgtaa
      661 ataaagtttt ccagtatctc ctctgttttc tctgtatgga tatatgtgta ttccggagtc
      721 ttactgaagg ttgtagtaaa tggattctgc ataaattcac ctcaatttat tatatgaaat
      781 tcaaaactat ttatattttt ttatactttc tattgctctt tatagttttt tataaatctt
      841 tatacttttt cttttatttt atactttttt atactttttt gcaatagttc ttccgacctg
      901 tatataccgt taaaggaatc accgcttttt gcacccttga caaacccctt cttaaacagc
      961 tataatatcg tcaattgaag cacaattaca tttcaacagg ctttgatggg aacaagtaca
     1021 tttctgcaat acttacagag agctgccggc aggtgagagg cgcaaggaaa cagatctgga
     1081 atacatcccg gagctttgca ccgaacgtca taccggatgg ttaaatatcc ccggttcgat
     1141 taataagccg caacgtattg gcacacgtta tagtgccgga gtgttagcag aatcggctat
     1201 tttcactata taatatatgt cgaataatgt gtgacaactc acggaggcat tcctgtgcga
     1261 gcaggatgca aattaaggtg gtaacgcgag aaatccccct cgtccttaaa tggatgaggg
     1321 ggatatttta tttttcagaa tacagattca tcttatcagg aggaaaatca tgacaattta
     1381 tgacgaatta gttgcccgcg gccttatagc gcaggtaaca gacgaaaaag aaatcaaaga
     1441 gcttataaac aacggaaagg ctaccttcta catcggcttt gatcctacag cagacagcct
     1501 tcacgttgga cacttcatgg ctctttgcct tatgaagaga cttcagatgg caggaaacaa
     1561 gcctatcgtg cttatcggtg gaggtacagc acagattgga gatccatcag gacgtacaga
     1621 tatgcgccag atgatgacaa cagagactat caaccacaac gtagagtgct tcaagaaaca
     1681 gatgagccgc ttcattgact ttggcgaggg aaaggctatc atggtaaaca acgctgactg
     1741 gctgatggac ttaaactacg tggatgtgct ccgcgaggtt ggtgctcatt tctcagtaaa
     1801 ccgtatgctt acagctgagt gctacaagca gcgtatggaa aagggtcttt ccttccttga
     1861 gtttaactac atgatcatgc agagctacga tttctacaca cttttccaga aatacggctg
     1921 caacatggag ttcggtggag atgaccagtg gagcaacatg ctcggtggaa cagagcttat
     1981 cagacgtaag cttggaaaag atgcatatgc tatgacaatc aatctactcc taaactctga
     2041 gggcaagaag atgggcaaga cacagtccgg tgcagtatgg cttgatccta acaagacaac
     2101 acctttcgag ttcttccagt actggagaaa cgtttctgat gccgatgttc ttaaatgtat
     2161 cagaatgctc accttccttc ctctcgagga aattgacaag atggaaagct gggagggcgc
     2221 acagctcaat gaagctaagg aaatcctggc attcgagctt acaaagcttg ttcacggtga
     2281 ggaggaagcc gcaaaggcta aagaggcaag ccatgcactt ttcgcaggcg gagccaacaa
     2341 tgcaaatatg cctacagtta ccgttacagc tgaggacttc cctgacggag agcttgatat
     2401 catctctgtt ctcgtaaaag caggactttg cgattcaaga ggagacggcc gcagaaatat
     2461 ccagcagggc ggtgtttccg ttgcagatga gaaggtaact gatatctcta caaaatacac
     2521 tctcgacgat ttcaagggcg agggtctcat tatcagacgt ggtaagaaga aatttgccaa
     2581 ggttgtagct gaatagacat agaatccccg gtagttacta ccggggattt ttacattcag
     2641 ccatatttta ttttgaaaat tcatttgcag gaaattcata tggcacaaat ttactgctac
     2701 atataactgt atactccgtg tctcccgaaa ccgttttttg ttctgtgcca acaccctctc
     2761 cacaaatcca gtatgaacca tcatttttat tctttagcat aatatcccca cactaaatta
     2821 gcatagtgta caatagccat atgttgatag ctgacaggcc ttgggtagct gtaatccata
     2881 aaacttctaa aacagctccc caatccagtc caaaaactgg aaaaattctg gaaaaaagta
     2941 gctgaaaccg ctgaaatttc taaaacagct cccctaaccc agcgaaacag aatggcttat
     3001 tatccatcat ttatatatat taaaccctat tttttcctca tcttcccttg acttatcatt
     3061 aataagttaa tataatttat ctaattgata catcaacgct ttaaagtgtt gacaaaagga
     3121 ggaattatca tgaaaaagag atctaaaatt ttagcttcac ttgttcttgc cggaacaatg
     3181 gttcttggac ttgccggatg cggagattcc ggttcagcaa acggtggcac acagtcaggc
     3241 aagaactcag atgccaaata tcaggtaggt atctgccagc tcgtacagca cgaggctctc
     3301 gatgccgcta caaagggctt taaggacgca ttaaaggaca agctcggaga cgatgtctca
     3361 tttgacgagc agaatgccgc aggagatgct tctacatgct ctacaatctg caccaagttc
     3421 gtcaccaacg actatgacct tatccttgga aatgctacag cagctcttca ggccgcttca
     3481 gcagctacag actctattcc gattctcgga acatctatca cagattacgc tacagctctt
     3541 gatatgtcag actggtcagg cacaacagga aagaatattt caggtacaac agacctcgct
     3601 ccactcgatg agcaggctaa atgcatcaag gagctcttcc cggatgcaaa gaacatcggt
     3661 atcatctact gctcatctga gccaaacagt atctaccagt caacaactat taccaaatac
     3721 cttgaggacg agggatacac tgtaaaagag tacaccttct ctgattcaaa tgatgttgct
     3781 gcagtaacac agagcgcatg tgatgcatct gatgcaatct acatcccaac tgacaacact
     3841 gctgcttcat gtacagaggc tatcaacaat gtagcttcac aggctaagaa acctatcttc
     3901 gccggcgagg aaggaatcgc taagggctgt ggtgtggcaa cactttctat cagctactat
     3961 gaccttggat acacaacagg tgagatggct tacgatatcc ttgtaaatgg tacagacgtc
     4021 aaaactatgg aagttaagag cgctcctaag ttcactaagg agtaccttcc ggaccgcgcc
     4081 aaggagcttg gaatcacagt tccggacggc tatgaggaaa tctcagctga ataaacagct
     4141 gcactatgga ggtatttaat aaaatgattc taatgaatta ttttgcaacc ttaaatccgg
     4201 ttgcattcac caatgccctt ccgggcaata tagcccaggg cctgatatgg ggtatcatgg
     4261 cactcggtgt ttatatcact ttcagactgc tggacttcgc agatctgacg gttgatggct
     4321 catttgcaac aggtggtgcc gtaaccgtta tgatgacgat taacggctac aatatctggg
     4381 tttcactgct ttgtgcatgt attgccggtc tgctcgcagg actctgcaca ggtctcttac
     4441 atacaaagct cggcattcct ccgattctgg caggaatcct tacacagata gctctctact
     4501 ctatcaactt aaatatcatg cagcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnggc tgcaaccctg caatgagcaa ggctcagggt
     4741 atcaacatcg acaacatgaa gctcattggt cttgcgcttt caaatgcaat tgtagcactc
     4801 tcaggcggcc tgctttcaca gtactcaggc ttttccgatg taaacatggg ccgtggtgga
     4861 atcgtcatcg gtcttgccgc agttatcatc ggtgaggtgc tcggcgatgc aatcctcgga
     4921 aagcacatga atttcatggg aaaacttatc tttgtcatat ttggtggaat tgtttactac
     4981 attgtaatcg gaattgttct gtggctgcag atgccaagtg atgacttaaa gctcttcact
     5041 gcaatcatcg tggcaatttt ccttgcagtg ccttacctaa agggcaagag caaggcctcc
     5101 tttaaaaagg ccggcaaacg ctctgctgca gcacttaacg caaaggaggg caaataaatg
     5161 ctagagatta gaaacgtaca taaaaccttc aatccgggca caatcaatga aaagcatgcc
     5221 cttaatggtg taaacctaaa gcttgaggag ggcgatttcg tcactgttat cggtggaaac
     5281 ggtgccggaa aatcgaccat gctcaatgcc gttgcaggca catggcctgt tgacgatggt
     5341 tcaattctca tcgatggagt ggatgtcaca ggactccctg agttcaagcg tgcatcattt
     5401 ctgggacgtg tgttccagga tccaatgaca ggaactaccg caaccatgca gattgatgaa
     5461 aacctcgcac ttgccgctcg ccgcggaaag cgccgtggtc ttggctgggg tattacaaag
     5521 gctgaaaagg agcaattcca cgagctttta aaggagcttg acctgggact cgaggatcga
     5581 atgacctcaa aggttggtct gctctcaggt ggacagcgtc aggctgttac actgctcatg
     5641 gcttcactcc agaagccgaa ggtgctgctt ttggatgagc acaccgctgc cctcgaccct
     5701 aagacagccg caaaggtgct cactctcaca gacaaaatca tcaaggagaa cagcctgaca
     5761 gccatgatgg tcacacacaa catgcgtgat gccatcgctc acggcaacag actcatcatg
     5821 atgaataacg gacaggttgt ccttaatatt tcaggcgaag aaaagaaaaa cctcacggtg
     5881 gaggatctgc tcatgaagtt tgaggaggtt tccggcgagg aatttgcttc tgataaggct
     5941 ttgcttggat agacatatct ataataataa caaaaatttg cagctcaata cacgagctgc
     6001 aattttttgt tatatacttt ttatatgagt tctcccaccg gcttaatcat atcag
//
LOCUS       DPUF01000179           12355 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_19127, whole
            genome shotgun sequence.
ACCESSION   DPUF01000179 DPUF01000000
VERSION     DPUF01000179.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12355)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12355
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_19127"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="DHV81_06360"
     CDS             <1..840
                     /locus_tag="DHV81_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF342 domain-containing protein"
                     /protein_id="HCI94087.1"
                     /translation="IILPILEINGDVDVGTGNIDFVGDVVIHGSVKTGARIRAAKSIT
                     IDGVCEGCVLEAGNDLILRNGMIGMGKARIIVKGNLFAKFMEYTDVEVDGFVEADSAI
                     NCNVVSNDKVIFNGGHASIVGGKVYGCAGIEVQNLGNDAFIKTEVHVGVHKKIKIKIA
                     ELEKLVDQKQMLLNNINAGIKQIEQMMGSAADGMNLEEKKLALVRAKIEKTAELTEDK
                     EELERLKGIVERSTGATVQVLEHVYPNVEVCINNSKLVTKEEFDKIEFKEKDKAVVML
                     SMK"
     gene            1118..2488
                     /locus_tag="DHV81_06365"
     CDS             1118..2488
                     /locus_tag="DHV81_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCI94088.1"
                     /translation="MICYKCGQDAGWNDKCPVCGADLSLFKRAIRISNSYYNDGLQKA
                     QVRNLSGAIISLRQSLKFYKYNIQARNLLGLIYYEMGEMVDALSEWVISANYQPDDNL
                     AKKYIDQIHNNRNQLETINQTIKKYNQALTYCKQDSKDLAVIQLRKVLSLNPKLVKGH
                     QLLALLYLEEGHLDKAKKALRDAGKIDTDNTTTLRYLKEVNRRLKEKGTERKKKNDDL
                     ISYQSGNETIIMPKRFRESSMWANILYIIIGLVVGVAVTCFLVVPGIKSQAKTDAKKQ
                     LLSANDTISTNGQTISGLNDQIDKLKKELEDEKKKNDGVSDQISTYEALLNAYVYYTT
                     NDVIKAGEALENINTSYLSDSAKQTYDTLNGSIADSYKEALYSQAYSSYSSGDYQSAI
                     PTFQKLVGMDEAYRDGSVAYYLAQSFRKSGDLASAKPYYQYVVDNYAGTEKARTSKNY
                     LAQEQQ"
     gene            2587..2895
                     /locus_tag="DHV81_06370"
     CDS             2587..2895
                     /locus_tag="DHV81_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HCI94089.1"
                     /translation="MIENYIKEADRVTYLMPKEVDHHCAGQIIAELDALIEGCGVRKI
                     VFDMKQTEFMDSSGIGVIIGRTKKLKYFNDSSVSVCNMSDRVDKLLKCAGIYTIVHKL
                     "
     gene            2939..3418
                     /locus_tag="DHV81_06375"
     CDS             2939..3418
                     /locus_tag="DHV81_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HCI94090.1"
                     /translation="MNAAKTNHISLSFDAISENEAFARMTAAMFMAQLNPTLDDVEDV
                     KTAVSEAVTNAIIHGYEMGDDNISSGQADHQAAEEKSCPQVHMNCSYIGRELYIEISD
                     SGVGIQDVAKAMEPLYTSKPQLDRSGMGFSFMEAFMDALTVTSEPGHGTTVKMKKIL"
     gene            3415..4089
                     /locus_tag="DHV81_06380"
     CDS             3415..4089
                     /locus_tag="DHV81_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HCI94091.1"
                     /translation="MKSDKISRLVTENINLIYLVMKRFRNRGVDREDLFQIGAVGLTK
                     AAQRFDESKGFAFSTYAVPLIIGEIQRFLRDDGMVHISRKILDDARKIAIIREKYRMS
                     ENSEISLEKMENISGMSKQDIIIAMEADKNASSIENESVLDYLDNKMSAEASDKPSET
                     DKMINHIAMQQAFNSLDDSERRLIMLRYMQDKTQKQVAEVLGRNQVAISRMEKNTLAK
                     LRRAMM"
     gene            4790..5576
                     /locus_tag="DHV81_06385"
                     /pseudo
     CDS             4790..5576
                     /locus_tag="DHV81_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743390.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     assembly_gap    4975..5069
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5725..7074
                     /locus_tag="DHV81_06390"
     CDS             5725..7074
                     /locus_tag="DHV81_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCI94092.1"
                     /translation="MSNTLLMLCIPFAGLLLCIAVMPLVKPEWWEKHQAHAVILWSLL
                     FAIPFALFYGAPKAVETVLECLIGDYLTFIVLLFGLFCVAGNIKLEGSLVGNPKVNVI
                     MLAVGTFFSSCIGTTGASMLFVRPIIQMNSWRKNKRHIMVFFIFLVSNIGGCLTPIGD
                     PPLLMGFSRGVSFFWSMRLFPVLVLNMILLLTIFYHLDKKAYQKDITKGLMPEVKENE
                     PLIRIKGAHNFLYIVMIVIAVILSGVLPKLSAFQNAAGEVIGIPIFREVTLTVPAIIE
                     IAIILLAALLSFKTTPKEVRVQNHFTWGAIQEVAVLFIGIFITMQPALMVLKSAGSGL
                     GITKPFEMFWATGALSSFLDNTPTYLVFLTTAGAMHFTTGVATTLGVVPVKMLMAISC
                     GAVFMGANTYIGNAPNFMVKSLSDENGINMPSFFGYMWWSLRYLIPVFIIDMIIFFL"
     gene            7087..8082
                     /locus_tag="DHV81_06395"
     CDS             7087..8082
                     /locus_tag="DHV81_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HCI94093.1"
                     /translation="MEFYDEKIKALAIEIYELDAEVYKKLGSSLGRVFGGEKDICIRN
                     IMEDLHSRQNSSRIRSELALISNMARTIPDHDTRHDIMVRYNNIVEEIAELPTGFADG
                     DILDPKIADLNTVNLKDRFKTGDHLIICIGRSCGCGGNEIGFALADKLRMNFYDVSVM
                     NEVFRQKDGEEAAQATATFQKKERMGIKKRIKAFRKYHGLSSEDVLFFDTSKFLVEKA
                     KQEDFIVMGRFADAILTNNHIPHISIFITAPEKRRIKRFLEINKNVTPNQAKKWIESE
                     DKRHLKTYKFYTGRKWSKASNYDICINSASYGIKGSIELIIRMLQLDDRVKQLIE"
     gene            8210..8704
                     /locus_tag="DHV81_06400"
     CDS             8210..8704
                     /locus_tag="DHV81_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94094.1"
                     /translation="MFEIGDYVLNATNGICKISEIVELDMSGDKQLKSYFLLRPVEEE
                     NDRVYIPVDNADKRIRKVITQDEALAVLDRVPEIEALAVNNEKERETRYKEAVRSCEP
                     DSVISLLKCLHIRNEQRAQAGKKSTAVDERYGKMAEHNLNAELAFVLEKDKQEIKDII
                     HDML"
     gene            8919..9620
                     /locus_tag="DHV81_06405"
     CDS             8919..9620
                     /locus_tag="DHV81_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCI94095.1"
                     /translation="MNNMNINSAIADTLDIGNTDTEKKTGLIGFYNYTVVLTYIGMWI
                     GFLGVMLAIKDRYVGSLICLMLAGVCDMFDGTIASTKKDRTRDERTFGIQIDSLSDII
                     CFGVLPAVWVFCVTPKISVAFIAIFVFILCGLIRLAYFNVDEANRQAATAERRQYYLG
                     LPITSSALAIPAVYLIGEFTAIHTQTIAALALLIIGICYVLPVKLKKPSLFGEVLLVG
                     IGAVEFCILIAEVLI"
     gene            9620..10441
                     /locus_tag="DHV81_06410"
     CDS             9620..10441
                     /locus_tag="DHV81_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCI94096.1"
                     /translation="MAKKSFVDWLYTTAPGRVTLKVLLHTGALKLGEIVARSPLSKPF
                     ISRYIKNNNIDMSDFKGQKYNSFQDFFSRKRDDIEVDRQAEHLISPCDGYLSAYRIKS
                     NNSFHIKGSWYKVTDLVTDKKIAKEFVGGDCVILRLCANDYHHYCYIDDGFQGRNHFV
                     KGLLHSVQPIALENAPVYRQNRRMWTILDTVNFGKVAQIEIGALLVGGIVNDHENVMM
                     RKGAEMGHFELIGSTIVLLFKKGHIDLLPEIKECIKGDKEVRVIQGQHIANRTVF"
     gene            10446..11081
                     /locus_tag="DHV81_06415"
     CDS             10446..11081
                     /locus_tag="DHV81_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidic acid phosphatase"
                     /protein_id="HCI94097.1"
                     /translation="MLPKYSRIPLLFTVVLNFSVYWGARAIAGGFTHHNIETSLDDMI
                     PVVYPTVVIYFGCYIFWIIGYLYNAAMDKRHCYRFLMADWLAKAVCFICYVVYPTTNT
                     RPEIVGSDIWAQLMRFLYSMDAADNLFPSIHCLVSWLCYIGIRGNKKVPQWIRTTFCI
                     CAIAVFISTLTTKQHVIYDVVAGVALVEVTYRVSKLIVKKAVKKGKSNTEA"
     gene            11143..11805
                     /locus_tag="DHV81_06420"
     CDS             11143..11805
                     /locus_tag="DHV81_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCI94098.1"
                     /translation="MDDTQIEMLPMLGEFSSIASQYEHIMFYYESGIQQIVAKLQILN
                     NEFKNNHERNPIENIKSRVKSLDSIIDKMKRKGIPLTTNAMKREIKDIAGVRVICPFI
                     SDVYQVANMLVNQADVEIVTIKDYIKKPKENGYRSLHMIVLVDVYFSDHKDKVPIEIQ
                     FRTIAMNFWASTEHQLRYKKSRIFTEEMQQELKKCADIMADADDKMQNLANVSGIVQP
                     IE"
     gene            12036..>12355
                     /locus_tag="DHV81_06425"
     CDS             12036..>12355
                     /locus_tag="DHV81_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HCI94099.1"
                     /translation="MNIQKFTQKSIEAVNDCEKLAYDYGNQEIEQEHLLVALLSQEDG
                     LIPKLIDKMEINVEHFTENAKRHLAARTKVSGSSAQVYVGNDLNKVLIHAEDEAKAMG
                     DEYVS"
BASE COUNT         3932 a         2131 c         3002 g         3195 t
ORIGIN      
        1 attattcttc ctattcttga aataaacggt gatgtggatg tgggaacagg aaatatagat
       61 tttgtcgggg acgtggtcat acatggcagt gtgaagaccg gagccagaat aagggctgca
      121 aagtccatca cgatagatgg tgtttgcgag ggctgtgtgc ttgaggccgg caatgacctg
      181 attctaagga atggcatgat tggtatggga aaggcccgga ttattgtaaa gggcaatctg
      241 tttgccaagt tcatggagta tactgatgtg gaggtagatg gctttgtgga ggctgattct
      301 gcaatcaact gcaatgtggt cagcaatgat aaggtaatct tcaatggtgg acatgcaagc
      361 atagtcggtg gaaaagtata tggctgtgca ggcattgagg tgcaaaacct gggtaatgat
      421 gcctttatca agactgaggt gcatgtgggc gtgcataaaa agattaagat aaagatagca
      481 gagcttgaaa agcttgttga tcaaaagcag atgctgctta acaatatcaa tgcaggcata
      541 aagcagatag aacagatgat gggcagtgcc gctgacggta tgaatcttga ggaaaagaag
      601 cttgcgcttg tcagggcaaa aattgagaaa acagcagagc ttaccgaaga taaggaggag
      661 ctggagcggc ttaagggcat agtggagcgc tcgacaggcg cgacagtaca ggttttggag
      721 catgtatatc caaatgtcga ggtgtgtatt aacaactcaa agctcgtcac caaggaggaa
      781 ttcgacaaga ttgagttcaa ggagaaggat aaggcagtcg tgatgttgtc gatgaaataa
      841 aaaaattgcg atatttttat taaatcttaa tggaaatata cttgcataaa ggggttactt
      901 tattataata tatagtgacc cttttttgat gggattattt agcaggtaat tatggaggca
      961 ggattatgat aaattttgat gaagaaataa agaactatga gccaagtcta gagatagagg
     1021 aggctgaatc tgcgatttac agccacgatc tgacagatgt cacagatatc attgcacagt
     1081 tattacgtga gcaggacaaa aggcagggtg aattaagatg atttgttata aatgcggaca
     1141 agatgcaggc tggaacgata aatgccctgt atgtggggct gatctttcac tttttaaaag
     1201 agctattaga atatccaact catactacaa tgatggttta caaaaggcac aggtgagaaa
     1261 tctgtctgga gcgattatct ctctcaggca gagtcttaag ttttataagt acaatatcca
     1321 ggcaaggaat ctgttaggtc tcatatacta tgagatgggt gagatggttg acgccttaag
     1381 tgagtgggtc atcagtgcaa actatcagcc tgatgataat ctggcaaaga agtatataga
     1441 tcagattcat aataacagaa atcagctcga aacaatcaat cagaccatta aaaaatataa
     1501 tcaggcttta acttattgca agcaggacag taaggacctt gcggttatac agcttcgaaa
     1561 ggtattatcc ttaaatccaa agcttgtaaa gggacatcag cttttggctc ttttatatct
     1621 ggaggaggga catctggata aggcaaagaa agccttacgc gatgcaggca agatagatac
     1681 agacaatacc actaccctca gatacttaaa agaagtgaac aggcgtctca aggaaaaagg
     1741 aactgagagg aaaaagaaaa atgatgattt aatttcctac cagagtggaa acgaaacaat
     1801 cattatgcca aagcgattca gggagagctc aatgtgggca aatatactct atattataat
     1861 cggtctggta gtcggagtgg cggttacgtg cttccttgtg gttccgggca taaaaagcca
     1921 ggcgaaaaca gatgcaaaga agcagctatt atcggccaat gatacgattt ccacaaatgg
     1981 gcagaccatc agtggactta atgatcagat agacaagcta aagaaggagc ttgaggacga
     2041 gaagaagaaa aatgatggag tatcagacca gatttccaca tatgaggctt tgctcaatgc
     2101 ctatgtatac tatacaacca atgatgtaat aaaggcagga gaagcacttg aaaatatcaa
     2161 cacaagctac ctgagtgact cggctaagca gacatatgat acgctcaatg gttcaattgc
     2221 agattcatat aaggaagcac tgtacagtca ggcatactca tcatacagca gtggcgatta
     2281 tcagagtgca atccctacat tccagaagct tgtcggcatg gatgaggcat acagggatgg
     2341 ttcagttgca tattacctgg cgcagtcgtt caggaagtca ggtgacttag catctgcaaa
     2401 gccatattat cagtatgtgg tagacaacta cgcaggcact gagaaggcca gaacatcaaa
     2461 gaattatctg gcacaggaac agcagtaaaa caactgctgc gaggcaataa ttaaatatag
     2521 aattacgaaa gacaccagat aatctccggt gtctttttta tatgagcagg aaaggaagag
     2581 tgtgacatga tagaaaatta tatcaaggaa gcagaccggg tgacatacct tatgccaaag
     2641 gaggtggatc accactgcgc cgggcagata atcgcagagc ttgatgcact tatagagggc
     2701 tgtggtgtca gaaaaatagt atttgacatg aaacagacag aatttatgga cagctccggc
     2761 attggggtga taataggaag aacaaagaag cttaagtact ttaatgacag ctcggtgtca
     2821 gtttgcaata tgtcggacag agtggacaag cttttgaaat gtgccggcat ttacactatt
     2881 gtacataagc tttaaaactg aattaaatta aaatataatc agacagaaag ggagcataat
     2941 gaacgcagca aaaacaaatc atatatcgct tagctttgat gcaatttcag agaatgaggc
     3001 atttgcgaga atgacagcag ccatgtttat ggcacaatta aaccccacac tagatgacgt
     3061 ggaggacgta aagacagcag tgtcagaggc ggtgacaaac gcaattatcc atggctatga
     3121 gatgggggat gacaatattt catccggtca ggcagatcac caggcagcag aagaaaaaag
     3181 ttgtccacag gtacatatga attgcagcta cataggcagg gagctttaca tagaaatatc
     3241 agatagcgga gtaggcatac aggatgtggc aaaagcaatg gaaccgttat acacatccaa
     3301 accacagctt gacagatcag gcatgggttt ttcattcatg gaagcattta tggacgcgct
     3361 cacagttaca tcagaaccgg gacacggaac aacagtaaag atgaaaaaaa tactatgaag
     3421 agtgacaaaa tcagcagact tgtcacagaa aatataaacc tgatttattt agttatgaaa
     3481 cgctttagaa acagaggcgt tgaccgggag gatttatttc agataggagc agttgggctg
     3541 acaaaggcag cgcagcgatt tgatgaaagt aagggctttg ccttttctac atacgcagta
     3601 ccgttgataa taggggaaat acagcgcttt ttacgtgatg atggaatggt acatatcagt
     3661 cgaaaaatac tggatgatgc aagaaaaatt gcaattatca gagaaaaata caggatgagt
     3721 gaaaatagtg aaatatcact tgaaaaaatg gaaaatatct ccggtatgtc aaaacaggat
     3781 ataataattg ccatggaagc cgataaaaat gcatcgtcaa tagaaaatga aagtgtgctt
     3841 gattatttgg acaataaaat gagtgcagaa gcatcagaca agccatcgga gacagataaa
     3901 atgataaatc acatcgcaat gcagcaggcg tttaactcac tggatgatag cgagcgcaga
     3961 ctgattatgc ttcgctatat gcaggataag acacagaagc aggtggcaga agtgcttgga
     4021 cgcaaccagg tggccatatc acgaatggag aagaatacat tggcgaagct tcgcagggcg
     4081 atgatgtgag tgggaagcag tggaggtttt gagcttccca cgtgcaggct gagaggatgc
     4141 cagggtggca tgtagtatat tcttcaggaa attatgaagt cagccccgag ctcatgtgcg
     4201 gatagattgt ggatatgccg gggctttttt gttattaggg aatatggaac ctcgtggcag
     4261 ttcataattt tcttcagaac acactgcatg ccttccggca aataaagtgt gtcaagtggg
     4321 aagcatcgta ggatgggagc ttcccacata aaggctgaga gcgccgagcc gtccgaaccg
     4381 caggtgagga ccggcacatt atatctcagc gtaagtggca taagccggtg gttgggcagg
     4441 cggtactgtc gggtgggcga atagcgcagc tgtttagtgt ctgtagaatt atgctgtaaa
     4501 gcacagaaat gccgaggcat ggacgccgag gcaaggagaa atcgagccat ggagggcgag
     4561 tttcgacggt ttcgtcgggc atagaaaacc tgcgggcata attcgtacag acacttaaca
     4621 gcgaagcgta gccagcacga cagtaccgcc tgccccaacc accggcttat gccacgtatt
     4681 attgaaatta ctggaaacta ttgaaaaaag aaaagtactt gcactcaagc cacaaagcat
     4741 attacaatgg gtggtaagga atataattgg attgtcgaaa ggaaaaagca tgaaacttcc
     4801 actcacatat gataactata tatttgacct ctacggtacg ctcgtggata ttcacacaga
     4861 cgagagtgac actgcaattt gggaaaagct cgccatgttc tatggctatt acggagcact
     4921 gtatgaggca aaggagctta aggagcgcta tgagacactt gtaaagagca gcgannnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aagcatcacc ggagatacat atagaggatg
     5101 tatttgaaaa gctttatgag gaaaaggacg tgaatccgac aaaggagctt tcagtgcata
     5161 caggtcagtt tttcagggtg atgagcaccg aatacataaa gctttatccg ggcacaaagg
     5221 agatgcttaa ggaacttaag aaggcgggaa agaatgtata tctgctttca aatgcgcagc
     5281 gtatatttac agcctatgag atgagaagac ttgatatctt tgatttgttt gatgacgtat
     5341 ttatctcatc tgattataat accaaaaagc ctgatatcag attttataag gagctgataa
     5401 ataagcatga tattgacgta tcaaaatcgc tttttatagg aaatgattcg actaccgata
     5461 tcaagggtgc aaaggaatgc cggatggatg ccttttatgt gaaatcgaat atttcaccaa
     5521 aggatgatat ggctcatgat gcagactata ttatagataa ttttaccaac tggtaatgca
     5581 ttattgtttg taaaaatgtg aatattacat aaaaaacata tataaattaa aatcttttta
     5641 taaacggttg caaataaaaa ataatggtgt aaaatgaact agatttgaat tagtttattt
     5701 tttaacaaag tagaggtgaa aagaatgtca aacacattat taatgttgtg tatcccattt
     5761 gcgggcttat tactgtgcat agcagttatg ccgcttgtga agcctgaatg gtgggagaag
     5821 catcaagctc atgcagttat cttatggtca ctgctttttg ccattccgtt cgcgctgttc
     5881 tatggagcac caaaagccgt agagacggta ctggagtgtc tgataggtga ctatctgacc
     5941 tttatcgtgt tgctctttgg actgttctgc gtagccggaa atatcaagct ggagggaagt
     6001 ctggtcggca acccaaaggt aaacgttatc atgcttgctg ttggaacttt tttttcaagc
     6061 tgtataggaa ctacaggagc cagtatgctc tttgtaaggc cgattataca gatgaactca
     6121 tggagaaaaa acaagcgtca catcatggta tttttcattt ttcttgtgtc aaatatcgga
     6181 ggatgtctta cacctatcgg agatccgcca ctgctcatgg gattttcaag aggagtatcg
     6241 ttcttctgga gcatgaggct gtttccggtt ctcgtattaa atatgatact gcttcttacc
     6301 atattttatc atttggacaa gaaggcatac cagaaggata tcactaaggg acttatgcct
     6361 gaggtgaagg aaaatgagcc tcttatcagg ataaagggtg cccacaactt tttatatatc
     6421 gtaatgattg ttatagctgt tatactaagc ggtgtgcttc ctaagctttc tgcattccag
     6481 aacgctgcag gagaggttat cggtattccg atattcaggg aggttacact cactgtgcct
     6541 gcaattattg agattgctat tattctgctt gcagccctgc tttccttcaa gacaacaccg
     6601 aaggaggttc gtgtgcagaa ccatttcaca tggggagcaa ttcaggaggt tgcggtgctt
     6661 tttatcggaa tcttcatcac catgcagcct gctcttatgg tattaaagag tgcaggttca
     6721 ggtcttggaa tcacaaagcc gtttgagatg ttctgggcaa caggagcact ttcaagcttc
     6781 ctcgacaata caccgacata tctggttttc cttactacag cgggagccat gcactttact
     6841 acaggtgtcg caaccacact gggagtggtg ccggtcaaga tgcttatggc aatttcgtgt
     6901 ggcgctgtat ttatgggagc caacacatat attggaaatg caccgaactt tatggtcaag
     6961 tcattatcag acgaaaacgg tatcaacatg ccatcgttct ttggttacat gtggtggtca
     7021 ttgcgttatc tgatacctgt tttcatcata gatatgatca tattcttttt ataggaggga
     7081 tgaattatgg aattttatga cgagaaaatt aaagctcttg ccatcgaaat ctatgagctt
     7141 gatgccgaag tatacaagaa attaggctca tccctcggca gggtatttgg tggcgagaag
     7201 gatatatgta tccgtaacat catggaggat ctgcattcaa ggcagaacag ctcaagaatc
     7261 cgaagcgagc ttgccctgat aagcaacatg gcaagaacaa tacctgatca cgatacaagg
     7321 catgatatca tggtcagata caacaacata gtagaggaga tagcggagct cccgacaggt
     7381 tttgctgacg gagatatcct cgatccaaag atagcggatt taaataccgt aaacctgaag
     7441 gatcgtttca agacgggcga tcatctgatt atctgtatag gacgttcctg cggatgtggt
     7501 ggaaatgaga ttggatttgc acttgcagac aagcttcgta tgaatttcta tgatgtgtct
     7561 gtcatgaatg aggtgttcag acagaaggat ggagaggaag cagcccaggc tacagctacc
     7621 ttccagaaaa aagagcgaat gggaatcaaa aagcgcataa aagcttttag aaaataccac
     7681 ggactttcat cagaggatgt gcttttcttt gatacaagca agttccttgt agagaaggca
     7741 aagcaggagg acttcattgt tatgggacgt tttgcagatg caattctgac aaacaatcat
     7801 attcctcaca taagtatttt tatcacagca ccggagaaga gaagaatcaa gcgtttcctt
     7861 gagataaaca agaatgtgac tccgaaccag gcaaagaaat ggatagaatc tgaggataag
     7921 agacatctga agacctacaa attctatacc ggcagaaagt ggagcaaggc aagtaactat
     7981 gatatatgta tcaacagtgc cagctacggt atcaagggct caatagagct tatcatccgt
     8041 atgctccagc tcgatgaccg cgtgaagcag ctgattgagt aaatttttaa agcgtgtttt
     8101 attgacgtaa aacacgcttt ttttatatat ttgacatttt tgaaaaaatc ctcttacaaa
     8161 attcgtgaaa aagtgataaa atgataacgt ataacgaaag aggtgtgata tgttcgaaat
     8221 aggtgattac gtattaaatg ctacaaatgg aatttgcaaa ataagcgaaa tcgtggagtt
     8281 agacatgtca ggagacaagc agctcaagtc atatttttta ctccgccctg ttgaggagga
     8341 gaatgacaga gtgtatatac ctgttgacaa tgctgataag aggatacgta aggtaataac
     8401 acaggatgag gcactggcgg tgctcgatag agtacctgag atagaggcgc tcgcggtaaa
     8461 caatgagaag gagcgcgaaa cgcgctataa ggaggcagtc agaagctgtg agccggatag
     8521 tgtgatcagt ctcttaaagt gtttacatat cagaaatgag cagcgtgcac aggcaggcaa
     8581 gaagtctaca gccgtcgatg agcgctatgg taagatggca gagcataatt taaatgcaga
     8641 gcttgcattt gttcttgaaa aggacaagca ggagattaaa gatataattc atgatatgct
     8701 atagtatgtg ggcagaatca gaaagcgggt tctgccttgt tttttgctaa aaataagaga
     8761 tattaagaaa agtttaaaca aataattaat gagtttatgt catgaatatg ttattatata
     8821 tcaggtaagg gcatataata ttaccggtta agggtaaatg agaaaactta tcatgtttta
     8881 tctgataaaa acaaaagaat gaaagtaagg gaatcaatat gaataatatg aatattaatt
     8941 ctgcaatagc agatactctg gatataggga atacagacac agagaaaaag acagggctca
     9001 taggctttta taattacacg gtagtgctta catacatagg catgtggata ggcttccttg
     9061 gagttatgct tgccataaag gataggtatg tgggttcact aatctgcctt atgctggcag
     9121 gtgtctgtga tatgtttgac ggcaccattg catccacaaa gaaggatcgt accagggacg
     9181 agcgcacatt tggcatacag atagattcgt taagtgatat catatgcttc ggcgtgcttc
     9241 cggcagtgtg ggtattttgt gtgactccaa agatatcagt tgcatttatc gccatatttg
     9301 tattcatact ttgcgggctt atccgtctgg catactttaa tgtggatgag gccaacaggc
     9361 aggcagcgac agcagagcgc agacagtatt atctggggct tccaatcacc tcaagtgcgc
     9421 ttgctatccc ggcggtatat ctgattggag agttcacagc tatacataca cagacaatcg
     9481 cggcacttgc tcttttaatc attggcattt gctatgtgct ccctgtaaag ctcaaaaagc
     9541 catctctttt tggagaggtt ctgcttgtag gtataggagc agttgagttt tgtattctca
     9601 ttgcggaggt tcttatttaa tggcaaaaaa atcgtttgta gactggcttt acaccacggc
     9661 acctgggcgt gtgacactta aggtgcttct gcatacaggc gcacttaagc tcggagaaat
     9721 agtagcacgc tccccgctgt ctaagccgtt tatcagccgg tatataaaga ataacaatat
     9781 agacatgtct gattttaagg gtcagaaata taacagcttt caggactttt tctccagaaa
     9841 gcgtgatgat attgaggtgg acaggcaggc tgagcatctt atcagtccgt gtgacggcta
     9901 cctgagtgca tacaggataa aaagcaacaa cagctttcac atcaagggtt catggtacaa
     9961 ggtgacggac cttgtcaccg acaaaaaaat tgcaaaggaa tttgttggag gagactgcgt
    10021 gatattgcgt ctttgtgcca acgattatca tcattactgc tatatagatg atggatttca
    10081 gggcaggaat cattttgtga agggactgct tcacagtgta cagcctattg cactggaaaa
    10141 tgcacctgta tacaggcaaa acaggcgtat gtggactatt ctggatacag ttaattttgg
    10201 caaggtggcg cagattgaga taggtgcgct gcttgtggga ggcatagtca atgaccacga
    10261 gaatgttatg atgagaaagg gcgcagagat ggggcacttt gagctgattg gctccacaat
    10321 agtgcttttg tttaaaaaag gtcatattga cctgcttccg gaaattaaag agtgtataaa
    10381 aggagacaaa gaagtacgtg ttatccaggg acagcatatc gcaaacagga cagtttttta
    10441 acagaatact tccaaaatac agtcgtattc cgctgctttt tacagtggtg ctcaatttca
    10501 gtgtatactg gggcgcgcgt gctattgcag gcggttttac ccatcacaat atagagacct
    10561 cgcttgatga tatgataccg gttgtgtacc ctacagtagt catttatttt ggctgctata
    10621 ttttttggat tataggatat ctgtacaatg cagcgatgga taaaaggcac tgttatcgtt
    10681 tcctcatggc agactggctg gcaaaggcag tctgttttat ctgttatgtc gtgtatccta
    10741 caaccaacac ccgcccggaa atagtgggct cagacatatg ggcacagctc atgcgctttt
    10801 tatatagtat ggatgcagcg gacaatcttt ttccatctat acactgccta gtaagctggc
    10861 tgtgctatat aggcatccgt ggcaacaaaa aagtaccgca gtggatacgt acaaccttct
    10921 gtatttgcgc tatagcagta ttcatatcca cattgactac gaagcagcat gtgatatatg
    10981 atgtagttgc aggagttgcg ctggtggaag tgacataccg ggtttcaaag ctgatagtta
    11041 aaaaggcagt taaaaaggga aaatcgaata cagaagcata atatgtataa acagtataag
    11101 gtatacaatt agtaaaaaaa cctaaaacca aggagtataa acatggatga tacacaaata
    11161 gagatgctgc cgatgctggg cgaattcagt tcaattgcct cgcagtatga acacatcatg
    11221 ttctactacg aatcgggtat acagcagata gtagcaaagc tgcagatatt aaataatgaa
    11281 tttaaaaaca atcacgaaag aaatccaatc gagaatataa agagccgtgt aaagtcgctt
    11341 gacagcataa tagacaagat gaagcgaaag ggcattccgc tcaccaccaa tgccatgaag
    11401 cgtgagatta aggatatagc cggcgtcagg gtcatttgtc catttatatc cgatgtatat
    11461 caggtggcca atatgcttgt gaatcaggcg gatgtcgaga ttgtcacaat aaaggactac
    11521 ataaaaaaac cgaaggagaa tggctacagg agtcttcata tgatagtgct ggttgacgtg
    11581 tatttttcgg atcacaagga caaggtgccg atagagatac agtttcgtac cattgccatg
    11641 aatttctggg ccagcacaga gcatcagctt cgctacaaga agagccgtat attcacggag
    11701 gagatgcagc aggagctcaa gaaatgcgcg gatatcatgg ctgacgctga tgacaagatg
    11761 cagaatctgg caaatgtttc ggggattgtg cagccgattg agtaaaagag agtgatgtaa
    11821 atcactcttt tttgcttgta aatcaaaagt ttatatttat ttaataaaaa ttgttagcac
    11881 tctcacttga cgagtgctaa tctgcgtgct ataacatagt cacaaggaat attgaagaat
    11941 tatttaaagg attatttaga gataaaattt tgatattacc tgtatgacac ataagaatta
    12001 tatttatgga taacatagta gaagggagtg agactatgaa cattcaaaaa tttacacaga
    12061 aatcaataga ggcagtcaat gactgcgaga agcttgcata tgattatggc aatcaggaga
    12121 tagaacagga gcacctgctt gtggcattac tttcacagga ggacggactg ataccgaagc
    12181 tgattgacaa gatggagata aacgtagagc atttcacaga gaatgcaaag cgtcatctgg
    12241 cagcgcgcac aaaggtaagc ggtagcagtg cacaggtcta tgtgggcaat gatttaaaca
    12301 aggttcttat tcatgctgag gacgaagcaa aggcaatggg agatgagtat gtttc
//
LOCUS       DPUF01000180            3386 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_27597, whole
            genome shotgun sequence.
ACCESSION   DPUF01000180 DPUF01000000
VERSION     DPUF01000180.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_27597"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(330..2378)
                     /locus_tag="DHV81_06430"
     CDS             complement(330..2378)
                     /locus_tag="DHV81_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI94100.1"
                     /translation="MLYQISNGAVAFGDDVILHSIDFEIRNTEKIAIVGRNGCGKTTL
                     LKLISGEVEMEKLDSDESAFIAKAGNPEIGYLKQIAFDDPDVTLEQEVRKCFVKMDER
                     KAELARAAAELEHDYSDEKVARYTAMEEAFKDDGGYYYEKEYEVMIRKFGFSDDERKK
                     PIRDFSGGQQTKIAFIKLLLSKPDILLLDEPTNHLDVTTIEWLEGYLKSYPKAVVVVS
                     HDRMFLDNVVDVVYEIEYGTARRYPGNYTNFIARKKENYDKQMKDHIAQQKEIERLQR
                     MVTRFKGKPTKTSMAQSKQKAIDRMVIIEAPDKYDNKTFHANFQPEKETGNDVLYTSE
                     LAIGYDHPLSVVSLDLKRGEKLGILGGNGLGKSTFLKTIVGKIPALSGEYRFGTNVQI
                     GYFDQQMAMYTSNKTVLDDFWDEYPNLTETEARNALGAFLFSGEDVFKNVNMLSGGEK
                     VRLALCKILKTRPNVLVLDEPTNHMDIVGKETLESMLKDYKGTLIFVSHDRYFVKKVA
                     TQLLVFEDGTTNLYQFGYEQYQEKLDREAEESKNAYRGNAIFGGVISQNGSSQTGSDA
                     NRSTSQNAAAGNVGESTNANSAAQAGGMAVSSTGKAYYNPGKERSKIQKKVKKAEEDL
                     AVKEAKLDELKAELMKPEYQSSYSKLTEIQNEIDALEEEILIDMEAWEELSSQLEALG
                     "
     gene            complement(2409..>3386)
                     /locus_tag="DHV81_06435"
     CDS             complement(2409..>3386)
                     /locus_tag="DHV81_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI94101.1"
                     /translation="DYDFKPIESALGIDCFTQISNYKAIVKKDLMYNHNVSEKVRALS
                     KKLEAAKDEQEFFDVVTGFYKAYGVGMFGLNKAFRIEEHLDGGFSFMPINNMDAVMLD
                     DLIGYEIQKKKLTDNTEAFVQGKKANNVLLFGDSGTGKSTSIKAIVNQYYDDGLRMIE
                     IYKHQFKYLSKIIAAIKNRNYRFIIYMDDLSFEEHEIEYKFLKAVIEGGVETKPDNIL
                     IYATSNRRHLIKETWNDRNDQDNSNDKHHSDTVEEKLSLVNRFGVTISYSKPSQKEFF
                     NIVTELARKSGCTLSDEELCREANKWELSHGGISGRTATQFVNYIMANN"
BASE COUNT          741 a          880 c          689 g         1076 t
ORIGIN      
        1 gcccctctgt taattcgatg ttttttcctg aaattgttat tctcatgatg tccaacccct
       61 ttatcgttta tttgatacgt tgtctgtatc atgtaaatat tataccataa atttcttttt
      121 catcaatcat ttttatgcaa tttatataaa taatcaaggg tttatttacg gtttttcgcg
      181 aaatcgcttg atttcgctat gattagggat ttcatcagaa taaaaaacat caagtcgagt
      241 gattttttat acactatcgc aacatttctc aactacaaca gcacatctaa gctatcacaa
      301 cgtttctaag ctaccacagc gcatctaacc tatcccagtg cctcaagctg agaggaaagc
      361 tcctcccacg cctccatatc tataaggatt tcttcctcga gagcgtctat ctcgttttgg
      421 atttccgtga gctttgagta gcttgactga tactccggct tcatgagctc agccttgagc
      481 tcatcgagct ttgcttcctt gaccgccagg tcctcctcgg cctttttgac ctttttttgt
      541 atctttgagc gttccttgcc cgggttgtag tatgccttgc ctgttgatga gacagccatg
      601 ccgcctgcct gggctgcact atttgcattg gtgctttcgc ccacattacc tgctgcagca
      661 ttttgtgacg tgctccggtt tgcatcactg ccggtctgac tgctgccgtt ctgacttatc
      721 acaccgccaa atattgcatt gcctctgtat gcatttttgc tctcctctgc ctcacgatcg
      781 agcttttcct gatactgctc atagccaaac tgatagagat tggtcgtgcc gtcctcgaac
      841 acaagcagct gtgtggctac ctttttcaca aaatatctgt cgtgtgatac aaaaatcagt
      901 gtgcccttgt agtccttaag catgctctcc agcgtttcct tgcctacgat atccatatgg
      961 ttagttggct catcgagcac gagcacgttc ggtctggtct ttaaaatctt gcaaagcgcc
     1021 agtctgactt tttctcctcc tgagagcata tttacgttct tgaacacgtc ctcccctgaa
     1081 aaaaggaagg ctccgagtgc atttctggcc tcggtttccg tgagattcgg atactcatcc
     1141 cagaaatcat cgagcacagt cttgttgctt gtatacatgg ccatctgctg gtcgaaatat
     1201 cctatctgca cgttggttcc aaagcggtac tcgcctgaaa gtgcagggat cttacccacg
     1261 atagtcttta aaaatgtcga ttttccaagt ccgtttccac cgagtatgcc aagcttttcg
     1321 cctcttttca aatcgagcga cactacggag agcggatggt catagccgat tgcaagctct
     1381 gatgtataaa gcacatcgtt tccggtctcc ttttcgggct ggaaattggc atggaaggtc
     1441 ttgttgtcgt acttgtccgg cgcctcgata atcaccatgc ggtcgatagc cttctgcttt
     1501 gactgcgcca tggatgtctt ggtcggcttg cccttgaagc gcgtaaccat acgctgcaaa
     1561 cgctctatct ccttctgctg ggcgatgtga tccttcatct gcttgtcgta gttttccttt
     1621 ttgcgggcaa taaaattggt ataattgccc ggatagcgcc ttgctgtgcc gtactctatc
     1681 tcgtatacca catctaccac attgtcgaga aacatacggt catgggatac gacaaccact
     1741 gccttcgggt acgacttcag atagccctca agccactcaa tcgtcgtgac atcaaggtgg
     1801 ttggtcggct cgtccaaaag cagtatgtca ggctttgaca aaagcagctt tatgaatgct
     1861 atcttggtct gctgccctcc ggaaaagtca cggattggct ttttacgctc gtcgtcagag
     1921 aagccgaact tgcggatcat gacctcatat tccttctcat aatagtagcc gccatcatcc
     1981 ttgaaagctt cctccatcgc ggtatatctg gctacctttt catcggaata atcatgctca
     2041 agctctgccg ccgcgcgcgc aagctctgcc tttcgttcgt ccattttcac gaagcatttg
     2101 cgcacctcct gctcgagtgt cacatcagga tcatcaaacg caatctgctt cagataccct
     2161 atctccgggt ttcctgcctt tgcaataaag gcgctctcat cgctgtcgag cttttccatc
     2221 tcaacctcgc ctgaaatgag ctttaaaagt gttgtcttgc cacagccgtt tctgccgact
     2281 atcgctatct tttctgtgtt tctgatttca aaatctatac tatgcaaaat gacgtcgtcg
     2341 ccaaatgcga cagcgccatt acttatctgg tagagcatat actatccttt catgcttatg
     2401 cctgctgctt aattgttagc cataatgtag ttgacaaact gtgttgcagt acgtcccgaa
     2461 atgccaccgt ggctgagctc ccacttgttt gcctccctgc acagctcctc gtctgaaagt
     2521 gtgcagccgc tctttctcgc aagctctgtt acgatgttga agaactcctt ctgcgatggc
     2581 tttgagtagc tgattgtcac accaaaacgg tttacaagtg acagcttctc ctcaactgta
     2641 tctgagtgat gcttgtcatt gctgttgtcc tggtcattac ggtcattcca tgtctccttg
     2701 atcaggtgac ggcggttaga ggttgcataa atcagtatgt tgtcaggctt tgtctccacg
     2761 ccgccctcga taactgcctt taggaacttg tactcaatct catgctcctc gaatgagagg
     2821 tcatccatat atatgataaa tctgtagttt ctgtttttga ttgctgcgat aatctttgac
     2881 agatatttaa actgatgctt gtatatctca atcatgcgca gtccgtcatc ataatactga
     2941 ttgacaatgg ccttgatgct tgttgatttt ccggttccgc tgtctccgaa aagcagcaca
     3001 ttgttggcct ttttgccctg tacaaacgct tctgtattgt ctgtgagctt tttcttctgg
     3061 atttcatagc cgatcagatc atcgagcatg accgcatcca tattgttgat tggcatgaaa
     3121 gaaaagcccc cgtccagatg ctcctcaatc ctgaatgcct tgttgagacc aaacatgccg
     3181 actccgtatg ccttgtaaaa gcctgttacc acatcaaaga actcctgctc atccttagct
     3241 gcctcaagct tcttgctcag cgcccttact ttctcgctga cattgtgatt gtacataagg
     3301 tcctttttca caatggcctt ataattgctg atctgtgtga agcagtcgat tccgagtgct
     3361 gactcgattg gcttgaaatc ataatc
//
LOCUS       DPUF01000181            7401 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16831, whole
            genome shotgun sequence.
ACCESSION   DPUF01000181 DPUF01000000
VERSION     DPUF01000181.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16831"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="DHV81_06440"
     CDS             complement(<1..1753)
                     /locus_tag="DHV81_06440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94102.1"
                     /translation="MKNRNLLSIKKLAVLGICVSFVAGGCGKMTESSIPKLEEPVALN
                     EAYRPVEYGTIGSIQVLMATAVSRDYCNYYSANVNISQIYVEPGDTVNVGDVLAVCDV
                     DEAKKQLQECKDALAHENETYEISAQISDTQIKLWESQAADTDEFSTEQIEKKESTEE
                     DYNTQIATEQENQRYDKQLHEYRVQKINEQMALYQQIVDDGTLKAAHSGLVTYTKKLT
                     DGRSAAAYENIVIVSDTDDLVLDIKDTAINEYKYSDYDKKYVIVDGEKYDVTESQYTS
                     EVLVLSKINNRYPDVCVESREKLFLTAGQMYPVYFEKTKANQALVVGKDSIYKEGDTR
                     YVYVKNDEGGREKRIITTGEYDENYTQVTEGLKEGELVYYNSTDQVPTRYSKYTVTRS
                     DFDIANYGVSYSRSDAGTITETCDYEGQIVSVNVKQDDTVENGMLLYVVDTGEGKAAI
                     TEAKNEIDSENESYAHKIADYDKQVKDLSEGGSSAEENNKQDEKPDTAYEEQQLKLNL
                     QLLDLQKKLAGADHTYQLSRLQSAYDSISAGNDGKGNISVYAKNDGKVSKVSVKAGDN
                     VEEGSDILQITGNSTDVL"
     gene            complement(1750..2739)
                     /locus_tag="DHV81_06445"
     CDS             complement(1750..2739)
                     /locus_tag="DHV81_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94103.1"
                     /translation="MINKKKKYVGRLTLATAITSILFSGCGAAQKELAQTDRAFYEKI
                     SYQTVTVQSGSLTPQTTISLSAQGYTRFSYGATNTELTLEKVNVAVGDHVKKGDILVA
                     FKSGEIQKKIEDYSGQISQNKLLAEHYRAIMKIDDTQDYSSDIAQLDKDTEVAQLYLD
                     EAKEKLSRYQITASEDGTITAMDNSLLAGVFEPGSNLITEISGNGNYEADRPEGYDFN
                     VGDIYTATASDIEFELKVKEVDDKKVVFEPVSDMSAVSDAQIFSMEVTRPAIENAVYV
                     QKDAVHEKDGRYFVYTLDENGYRQAVWISAGDQVGDYRIITEGLKSGDEVVLQ"
     gene            complement(2881..>4041)
                     /locus_tag="DHV81_06450"
     CDS             complement(2881..>4041)
                     /locus_tag="DHV81_06450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94104.1"
                     /translation="TKEFKEKVIKAYFGDSQVYYYDYAHMTKKELEEIIETLDRDNNS
                     LSDDASEEILKWHDDMKTECTKALETAADVWTEATEYDSCDDFVGKIGDTWFHLQFSR
                     PDDDNSVLSGIQSYPLDRCVKSVSDGDPDERSTKFDDGYVKQYYGPKSLKEYDTVRYL
                     NGDFIDGDLPVSDVNEANISFDEAKKKAGDFMQAIGIESMIKTGESDCEWEGYNKTED
                     NVYKEGDVHRAIWGYALEYSLGADDVVYSKSLNVFDYSAYNKFCPMDCPGYETGSFNV
                     NEYGVYRFDIAEPVSIVKKQQKVELLPVSNIYKIARNELESHPDKYIVEADTQYRYLT
                     LGYIRVKDSSDSQRFSYIPAWSLELLQRGEVESCPVFVNAIDGTVIEPEQIF"
     assembly_gap    4042..4178
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4346..5740)
                     /locus_tag="DHV81_06455"
     CDS             complement(4346..5740)
                     /locus_tag="DHV81_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94105.1"
                     /translation="MDKRGNISSRNMRLFAAIMTLIEALVLTSLTGCAGKKVDYGVDT
                     EVKEQKNASKVSDLRTDKPWVENITVQTKKGDVTLGIDAKILVPDCETMHVVGVEDVK
                     LDTEYKKRFLDAYFGGGEYYYHDLEHYTVDELNSYIDLKSRELNKMNSRDDMDDSTKQ
                     ELAQQMEQDLSGYRDLLTDAKISYTIASDLQSCDEYAGYKGDTFCEVTFEEHWVNAYV
                     FKLDGKYYGPPSFKEENYDNVSQTFQEDNLTDSAGADGNECTRSAAESKKLVQDFMGK
                     LGLSNQVERGECDCNWLGFNNGDENWESRSNTLWGYVYSYGTGIDGQVFKDSFDSSEC
                     DSMLREDTKDITLGDSIQLTVTDNGIIDVVIEYPVSVTSISGSVEMLSLKNIEQIIKE
                     QLKNNADKYELEINKSFNNMQLTYFRLKDKNETAKYSYVPVWSFSRLVDSQKKNVILV
                     NAIDGTIINSEDVF"
     gene            complement(5898..6797)
                     /locus_tag="DHV81_06460"
     CDS             complement(5898..6797)
                     /locus_tag="DHV81_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94106.1"
                     /translation="MSGETKEILDSNYGQNDVIINRLIDKMTLFDDNLMSLVFGQNIE
                     TTELLLRVIMERDIKVIDVRGQDELKNSVIGGRCITLDVHAIDVDGRHIDIEVQINAE
                     GSHVRRARYHSSMMDARMLGEGQEFKEIKDSYVIFIYDHDKFRKGLPFYHIQRRVDET
                     GEVFGDGSHIIYVNGRYEGNDDIGRMMKDFHQCRPEQIESETLSKAVAYYKEKEGRGA
                     MSEAVRQYAMEYAKEYAKEYAREYGEEQKEEGILQGKNNMLYSLVSKGRLKIDVAAEE
                     ANVSLGEFEKSMEEAGYKIPELV"
     gene            complement(6914..7110)
                     /locus_tag="DHV81_06465"
                     /pseudo
     CDS             complement(6914..7110)
                     /locus_tag="DHV81_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7120..>7401)
                     /locus_tag="DHV81_06470"
     CDS             complement(7120..>7401)
                     /locus_tag="DHV81_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94107.1"
                     /translation="IWMLEDGTIIMAKAHMKDISDMSEYKEVLQADDESQAENDLSAY
                     KGYFDYSGIDFSKLNLISETTAVEQKNNESYEVLQFLQENEYVYAIHEK"
BASE COUNT         1801 a         1889 c         1106 g         2468 t
ORIGIN      
        1 gcagtacgtc cgtggaattt ccggttatct gcaggatatc tgagccttcc tccacattat
       61 ctcctgcctt gacactcacc tttgaaactt ttccgtcatt tttggcatat actgaaatat
      121 ttcctttgcc gtcatttcct gcgcttatgc tgtcatatgc tgactgtagc cgggaaagct
      181 gataggtgtg gtctgcaccg gcaagttttt tctgcagatc aagcagctgt aagttcaatt
      241 tcaactgctg ctcttcatat gctgtatccg gcttttcatc ctgtttgtta ttttcttctg
      301 cgctgctacc accttcagat aaatctttta cctgtttatc gtaatctgcg atcttgtgcg
      361 cataggattc attctccgag tcgatctcat tttttgcctc tgttatggca gctttgccct
      421 ctccggtatc caccacgtaa agcagcatcc cattttctac cgtatcgtcc tgcttcacat
      481 tgaccgatac gatctgccct tcataatcac aggtttcggt tatcgtccct gcgtcagatc
      541 tgctgtagct cacaccgtag tttgctatat caaagtcaga ccttgttacc gtatatttgc
      601 tgtaccttgt cggaacctga tctgtagaat tatagtagac cagctccccc tcttttaagc
      661 cctctgtgac ctgtgtatag ttttcatcat actcacctgt tgttatgatg cgtttctcgc
      721 ggccgccctc atcatttttc acatagacat atctggtatc gccttcctta tagatggaat
      781 cttttcccac gacaagtgcc tgatttgctt ttgtcttttc gaaatatacc ggatacatct
      841 gtcctgccgt aagaaacagc ttttccctgc tttccacgca cacatccgga tagcgattgt
      901 ttatcttcga aagtaccaaa acctctgatg tatattgaga ctcggtgaca tcatattttt
      961 cgccatcgac tatcacatat ttcttgtcat aatcactgta tttgtactca tttatggctg
     1021 tatcttttat atccagcaca aggtcatccg tatctgagac aatgactata ttctcgtatg
     1081 ccgcagcaga ccttccatct gtcagcttct tagtgtaagt gacaagaccg ctgtgggctg
     1141 ccttaagcgt tccatcatca acaatctgct gatataaagc catctgctca ttgatctttt
     1201 gcacgcggta ttcgtgaagc tgcttatcgt accgctgatt ttcctgttct gtggcaatct
     1261 gcgtattgta atcctcttct gtgctctctt ttttctcaat ctgttctgtt gaaaattcgt
     1321 ccgtatccgc agcctgactc tcccataatt tgatctgcgt atccgagatc tgagcggata
     1381 tctcatatgt ctcattctca tgcgccagcg catccttaca ttcctgcaac tgtttttttg
     1441 cctcatccac atcacagact gcaagcacat cccctacatt tactgtatct cccggctcca
     1501 catatatctg tgaaatattt acattggcac tgtagtaatt gcagtaatcc ctcgaaactg
     1561 cggtagccat cagcacctgt atggagccga tcgtaccgta ttccacaggt ctataggctt
     1621 catttaacgc tacaggttct tcaagctttg gtatgctgct ttctgtcatt tttccacagc
     1681 cgcctgccac aaacgataca catatcccca gtactgccag tttttttatt gataaaaggt
     1741 ttctgttctt cactgcaaaa ccacctcatc cccgctttta agtccttctg tgatgatccg
     1801 gtagtctccc acctgatctc cggcacttat ccataccgcc tgtctgtatc cattctcatc
     1861 cagtgtgtat acaaaatacc tgccatcctt ctcatgtact gcgtcctttt gcacatacac
     1921 agcattttct atagcagggc gcgtcacttc catggaaaat atctgtgcat ccgatactgc
     1981 cgacatgtcc gataccggtt caaaaacaac ctttttatcg tccacctctt ttaccttaag
     2041 ttcaaactct atatcagatg ctgttgccgt gtatatatca cctacattaa agtcataacc
     2101 ttccggtctg tcagcctcgt aattgccgtt tccgctgatt tctgttataa gattgctgcc
     2161 cggttcaaaa actcccgcca aaagtgaatt gtccattgct gtgatcgtac catcctctga
     2221 tgccgtgatc tgatatctcg acagtttttc cttagcctca tcaagataaa gctgcgcaac
     2281 ctctgtatcc ttatcaagct gggctatatc gcttgaataa tcctgagtat catcgatttt
     2341 cataatagcc ctgtagtgct ctgccagaag cttattctga ctgatctgcc cgctgtagtc
     2401 ctcaatcttt ttctgtatct ccccggattt aaaagccacc agtatatcac cctttttcac
     2461 atgatcaccc actgccacat ttaccttttc aagcgtaagc tccgtgtttg tcgcaccata
     2521 ggaaaacctg gtatagccct gcgcgctcag actgatggta gtctgcggtg tgagtgaacc
     2581 gctctgtacc gtaactgtct ggtatgatat tttctcgtag aaagctctgt ccgtctgtgc
     2641 aagctccttt tgtgccgccc cacatcctga aaacaaaata gaggtaattg cagtggcaag
     2701 tgtcaaacgc cctacatatt ttttcttttt attaatcata tattttccac atcttttcca
     2761 aataaatatg aacctgatct attatctaaa aatgtaactc catgtataaa ctttttgtat
     2821 catgtattgc attttttatt atataatttc ccttagtctc catgcacact tctctctgat
     2881 tcagaatatc tgctccggct caatcactgt tccgtctatt gcatttacaa ataccggaca
     2941 cgattcgacc tctcctcttt gcagcagctc aaggctccat gccgggatat agctgaatct
     3001 ctgtgaatct gagctgtcct ttaccctgat atagccaagt gtcagataac gatattgtgt
     3061 atccgcttcc acaatatatt tatccgggtg gctttcaagc tcatttcttg caatcttata
     3121 aatatttgac accggtaaga gctccacctt ctgctgtttt ttcacaatgc ttaccggctc
     3181 tgctatatca aatctataga caccatattc atttacattg aaactgcctg tctcataacc
     3241 aggacagtcc ataggacaaa atttgttata agctgaataa tcaaatacat tcagtgattt
     3301 tgaatacact acatcatctg cgccaagcga atattcaagc gcatagcccc atatggctct
     3361 atgcacatcc ccctccttat atacattatc ctcagtcttg ttatatccct cccattcaca
     3421 gtcgctttct cctgttttaa tcattgactc aattcctatt gcctgcatga agtcacctgc
     3481 tttcttcttt gcctcatcaa acgaaatatt tgcctcattg acatcgctta ccggcaggtc
     3541 tccatcaata aagtcaccgt ttaaatacct caccgtgtca tactctttca gtgattttgg
     3601 tccatagtac tgcttcacat agccatcatc aaatttagta ctcctctcat ccggatcccc
     3661 atctgaaacg cttttcacac atctgtcaag cggatagctc tggatgcctg atagcacact
     3721 gttgtcatca tctggccttg aaaactgaag gtgaaaccag gtatcaccta tttttccgac
     3781 aaaatcatca caggaatcat attctgtggc ctctgtccac acatcagctg ccgtttcaag
     3841 tgcttttgtg cactcagttt tcatatcatc atgccatttc aaaatctcct cagaggcatc
     3901 atccgacaga ctgttgttgt ccctgtcaag agtctcaata atctcctcaa gctccttttt
     3961 tgtcatgtgc gcatagtcgt aataatagac ctgtgaatca ccaaaatatg cctttatcac
     4021 cttttccttg aactccttgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gtcaatatcc tttacactgc
     4201 ttaccacatt ttgggatttt acctccgtgt ccacgccata atctaccttt ttgccggcac
     4261 agcctgcgag tgatatgagg acaagtgcct cgatgagtgt cataattgct tttttcttct
     4321 ttcccataat agttctccat aaattttaaa atacatcctc ggaattgatt attgtgccat
     4381 ctattgcatt tacaagtatc acatttttct tttgtgaatc aaccagccgc gaaaatgacc
     4441 atacaggcac ataactgtat tttgcagttt cattcttatc tttcagcctg aaatatgtaa
     4501 gctgcatatt gttaaagctt ttgtttatct caagctcata tttgtcggca ttgtttttta
     4561 gctgctcctt aattatctgc tctatattct ttaaagaaag catctccaca ctgccggata
     4621 tgcttgtcac actcaccgga tattctataa ccacatcaat tattccattg tctgtaacag
     4681 ttagctgaat actgtcaccc aatgtaatgt ctttcgtatc ctcgcgaagc atagagtcac
     4741 attctgatga atcaaaacta tccttaaaaa cctgtccatc aatgcctgtt ccatacgaat
     4801 acacatatcc ccacaatgtg ttactgcggc tctcccagtt ttcatcccca ttattaaatc
     4861 ccagccagtt acaatcgcac tctccacgct caacctgatt tgacagtcca agctttccca
     4921 taaagtcctg cacaagtttt tttgactctg ctgcacttct tgtacattca tttccatcag
     4981 ctccggcact gtctgtcaga ttatcctcct gaaatgtctg cgagacatta tcataatttt
     5041 cctccttaaa tgaaggtggt ccgtaatatt ttccatcgag cttaaagaca taagcattta
     5101 cccaatgctc ttcaaatgtt acctcacaaa atgtatctcc tttatagcca gcatactcgt
     5161 cacagctttg taaatcagaa gcaattgtat acgagatttt cgcatcagtc agtaaatccc
     5221 gataacctga taaatcctgc tccatttgct gtgccagctc ctgttttgta ctatcatcca
     5281 tatcatccct gctgttcatt ttattcagct cacgggattt taaatctata taagaattca
     5341 attcatcaac tgtataatgc tctaaatcat gataataata ctctccacca ccaaagtacg
     5401 catctaaaaa ccttttcttg tactctgtat cgagcttcac atcctccact ccaacaacat
     5461 gcatggtttc acaatctggc accagtattt tggcatcgat tcccagggtt acatctcctt
     5521 tttttgtctg gacagttata ttttcgaccc atggcttgtc agtccttaaa tcagatacct
     5581 tagaagcgtt cttttgctcc tttacctccg tgtccacacc ataatccacc tttttgccgg
     5641 cacagcctgt gagtgatgtg aggacaagtg cctcgatgag tgtcataatt gctgcaaata
     5701 atctcatatt tcgtgaagat atgttacccc ttttatccat atgtagctct ccttttttgt
     5761 gctctctgtt gttataatga gtatatatgt cagttgtaaa ctttttgtat catacattgc
     5821 atttttatta taaaaacccg gtctgcacat acttatgcaa accgggtcta tatttaacgt
     5881 atcttgtaac tattaaacta taccaattca ggaattttat atcctgcttc ctccatcgat
     5941 ttttcaaatt ctccaaggct gacatttgcc tcttcggctg ctacatctat ttttaacctt
     6001 ccctttgaaa ccaatgaata caacatatta ttttttccct gaagaatccc ctcttccttc
     6061 tgttcctcac catactccct ggcatattcc tttgcatact cttttgcata ttccattgca
     6121 tactgtctta ctgcttcgct catagcacca cgtccttcct tttctttgta ataagccact
     6181 gcttttgaca atgtctcact ttctatctgc tccggcctgc actggtgaaa atctttcatc
     6241 atccgaccta tatcatcatt accttcatac cttccattta catagatgat atgtgaacca
     6301 tctccaaaaa cttcgcctgt ttcatcaaca cgtctttgaa tgtgataaaa tggcagtcct
     6361 tttctgaact tatcgtgatc ataaataaaa atcacatagg aatccttaat ctccttaaac
     6421 tcctgtcctt cccccagcat cctcgcatcc atcatactgc tgtgatatct ggctcttcta
     6481 acatgcgatc cttccgcatt aatctgcacc tcgatatcaa tatgcctgcc atcgacatct
     6541 attgcatgca cgtccaaggt aatacatctc ccgccaatca ccgaattttt taattcatcc
     6601 tgccccctga cgtctataac cttaatatca cgctccatta ttacgcgaag cagcagctcc
     6661 gtcgtctcaa tattctgccc gaatacaaga ctcatcagat tgtcatcaaa cagtgtcatt
     6721 ttgtcaatca atctgttgat gattacatca ttctggccat aattactgtc caaaatttct
     6781 tttgtctctc ccgacattat tctcctcctg tttagttgtg tgagcaggga gaatataata
     6841 tcgcatgata cgaaatctct tatcaatata aagcatccgc aaccctagtc cgcttcattc
     6901 acataagatt ttctcaatcg acatatccat tttccccact attgataggt aacttaaagc
     6961 attgaaaatt tccatgtctt tgtcgaaaaa taataagtgg ttctgccctc ttcaaagtga
     7021 tacgcaccga taaaaatgtg tactgtggtg tcgctcaaca gaaatataat gctctgaaat
     7081 aatagtaaca tagtgaaccg catttgtcat tacaattttt cacttctcat gaatcgcata
     7141 tacatattca ttttcctgta aaaactgcag cacctcatag ctttcattat ttttttgctc
     7201 cactgctgtt gtctcggata tcaggttaag ctttgaaaaa tctatcccgc tgtagtcaaa
     7261 atatcctttg taagcagaca aatcattttc tgcctgtgat tcatcatctg cctgcaaaac
     7321 ctctttatat tcactcatat cactaatatc cttcatatgt gcttttgcca taatgattgt
     7381 cccatcctca agcatccaga t
//
LOCUS       DPUF01000182            3855 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_17815, whole
            genome shotgun sequence.
ACCESSION   DPUF01000182 DPUF01000000
VERSION     DPUF01000182.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_17815"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="DHV81_06475"
     CDS             <1..420
                     /locus_tag="DHV81_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94108.1"
                     /translation="ISLLTICIGFAPGILFAVVFGLIAYLMIMAQNTEFEYSYFDGEL
                     RFAKIKNKSRRKRLGIYSMESVAAIAPAGDRSVYNYENGNEIKKIDYTSGQKDVPYYD
                     IVIKSPDENVLIKAELDDKFLTEVEKKYRSKVKRREE"
     gene            complement(421..840)
                     /locus_tag="DHV81_06480"
     CDS             complement(421..840)
                     /locus_tag="DHV81_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94109.1"
                     /translation="MDNPKSLFNNNTRVCENCKRPLPHDYKENLCPRCLDLALFHEVK
                     EFIRTHDVNEYEVAEHFKIPLRQVKSWIKDGRIEYKQSESASTIAGLHCQRCGAPVTF
                     GTLCPKCLRLLNGSKGYSTGYDAGKTKSKMYHLEGEE"
     gene            912..>1602
                     /gene="murI"
                     /locus_tag="DHV81_06485"
     CDS             912..>1602
                     /gene="murI"
                     /locus_tag="DHV81_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCI94110.1"
                     /translation="MSIAVFDSGVGGISVLKSLVEVMPNEDFIYYGDSANAPYGTKTL
                     EQVRALTCEHAKELILNQHAKGLVVACNTATSAAVRILREQYPDVPIVGIEPAVKPAM
                     LFMENPRVLVMATPMTIREEKLKKLMDRYRHLGEIIPLPCPGLMNFVERGDLYGDDVR
                     QYLEELLYSYSHGEIDAVVLGCTHYPFVRKMIQEVLGDRVRVFDGGNGTAREMKRRLA
                     EKGLLTDSTAAG"
     assembly_gap    1603..1728
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1952..2590)
                     /locus_tag="DHV81_06490"
     CDS             complement(1952..2590)
                     /locus_tag="DHV81_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCI94111.1"
                     /translation="MTKEKLAIEVIKRLKAAYPRTDCTLEYDEAWKLLVSVRLAAQCT
                     DARVNVVVVDLFKKYPSIEALADADVSDIEEIVRPCGLGKSKARDISACMRMLRDDFG
                     GLVPDNMTDLLKLPGVGRKSANLIMGDVYGKPAIVTDTHCIRLCNRIGLVDGIKGPKK
                     VEMELWKIIPPEESNDFCHRLVDHGRAVCTARTTPHCDMCVLNDICGSVVHS"
     gene            2707..3327
                     /locus_tag="DHV81_06495"
     CDS             2707..3327
                     /locus_tag="DHV81_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HCI94112.1"
                     /translation="MKLENMNNAYANKKCTNNIYINNRYAIEVTRKRIKNMYLRVKDT
                     DGTLSVSAPYGMSDTEIRKFVESKSDWIERTMQEMLVARQLKEQEPVLAPFMERELRR
                     ELKMQLQRFIDKWEPVMGVKSAGFTIKKMKTRWGSCNVKSHHLNFNLLLAKVPSECAE
                     YVVVHELTHLLEPSHNARFWSLMEYYLPESKKLRKQLAGFSAQDMI"
     gene            3365..>3855
                     /locus_tag="DHV81_06500"
     CDS             3365..>3855
                     /locus_tag="DHV81_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HCI94113.1"
                     /translation="MEFWDIYDKDKKPTGRTMKKNDWILKDGEYHLSVLGVIHRPDGR
                     FLITQRVMTKAWAPGWWEVSGGAAQAGESSFTAVCREVKEEVGLDVSQCPGGLAYTYH
                     RENPGEGDNYFVDVYRFELDFKESDVQIQTEEALGFKIATLDEIKELAAQGVFLHYDS
                     IKQV"
BASE COUNT         1064 a          728 c          925 g         1012 t
ORIGIN      
        1 ataagccttc tgactatctg catcggcttt gcaccgggta ttttatttgc agtagtattt
       61 ggacttattg catatcttat gataatggca cagaacacag agtttgaata ttcatatttt
      121 gacggagagc tgcgctttgc caaaattaaa aacaagagcc gcagaaagcg ccttggaata
      181 tacagcatgg agtctgtagc agcaatcgca ccggcaggtg acagaagtgt atacaattac
      241 gagaatggaa atgagatcaa gaaaatagac tatacctcag gtcagaagga tgtaccttat
      301 tatgatatcg tcatcaaatc gcctgatgag aacgtactta taaaggcgga gcttgatgac
      361 aagttcctca cagaggtgga gaaaaaatac agatcaaagg ttaaaagaag agaggagtaa
      421 ttactcctct ccttccaaat ggtacatttt ggattttgtt ttgccggcat cgtatccggt
      481 tgagtatcct ttgctgccgt ttaacagtct cagacatttg ggacaaagag tgccgaaggt
      541 aacaggtgca ccgcatcgct ggcagtgaag accggctata gtggaggctg attcagactg
      601 cttgtactcg atgcgtccgt cctttatcca gctctttacc tgtctaagcg gtatcttaaa
      661 atgttctgca acctcgtatt catttacgtc gtgggtacgg ataaattcct ttacctcgtg
      721 gaataatgcc agatcaaggc atcgtgggca gagattctct ttatagtcat gtggcagagg
      781 ccttttacag ttttcacata ctcttgtgtt gttgttaaaa agacttttag gattatccat
      841 aggtgcctcc ttaagcttgt tttattcatt atatcataac agattttata aagaaagtag
      901 ggacttttat aatgtcaatt gcagttttcg attcaggagt cggcggaata agcgtcttaa
      961 agagcctggt tgaagtgatg cctaacgaag attttatata ttacggggat tcagcaaatg
     1021 cgccttatgg taccaaaaca cttgagcagg tgcgagcttt gacctgtgag catgcaaagg
     1081 agctgatttt aaatcagcat gcaaaggggc ttgttgtcgc gtgcaataca gccaccagtg
     1141 cggcggtgcg tatattaaga gagcaatatc ccgatgttcc tatcgtaggg atagagcctg
     1201 cagtcaagcc tgcgatgctt tttatggaaa atccgagagt gcttgtgatg gctacaccta
     1261 tgactattag ggaggaaaaa cttaaaaagc ttatggacag atacagacac ttgggtgaga
     1321 ttattccatt gccttgtccg ggacttatga atttcgttga gagaggtgac ttgtacggtg
     1381 atgatgtgag acagtatctc gaggaacttt tgtattcata ttcgcatggt gagatagatg
     1441 cggttgtgct gggatgcaca cattatccgt ttgtcagaaa aatgatacag gaggtgcttg
     1501 gagatagggt gcgtgtgttc gatggaggaa acggcacagc gcgtgagatg aaaagacgtc
     1561 tcgcagaaaa aggtctgctg acagacagca ccgctgcagg tannnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cttttttaat
     1741 aaaatatttc taaaatgtac attgttagga cataattacc acatatactt gttaatgtaa
     1801 gaggaacaaa accttttaca ttattccctt tttattaata aacattttca taactaaact
     1861 cctcgagaaa ggatcgcgtt gttaccgcgt gtaacatcgc ggtcctttct cacgttttgc
     1921 atgcaggctg cggagccgca gccttactgt tttagctatg tacaacgctt ccacatatat
     1981 cgttcagcac acacatgtca cagtgcggtg tggttcgggc agtgcatacg gcgcgtccat
     2041 gatcaaccag cctgtggcag aagtcattgc tctcctcggg tgggatgatt ttccaaagct
     2101 ccatctccac ttttttcggg ccctttattc catctacaag tcctatacgg ttgcaaaggc
     2161 gtatgcagtg tgtgtcggtg acgatggcag gcttgccgta cacatcaccc ataatcaggt
     2221 tggcactctt tctgccgacc cccggcagct tcagcagatc ggtcatattg tcaggcacaa
     2281 gaccgccaaa atcatcgcga agcatcctca tgcaggcact tatatcacgt gccttactct
     2341 ttcccaggcc gcatggacgt acaatttctt ctatgtcgga aacatcagca tcggcgagag
     2401 cctcgatgga aggatatttt ttgaacaaat caaccacgac tacatttacg cgggcatcag
     2461 tgcactgggc tgcgagcctg actgatacga gcagcttcca ggcctcgtcg tactcaagag
     2521 tacaatcggt gcggggatat gcggctttta gtctttttat tacttcaatg gctaattttt
     2581 cttttgtcat atgagcctcc ttatatttgt tttgtaacta ttcagtaact ttattttata
     2641 gtatacaaaa atgcgcggag attgtaaaat ggatttacgt ggagcggatt acgtgagagg
     2701 aacggtgtga aactggaaaa tatgaataac gcatatgcaa ataaaaaatg tacaaataac
     2761 atatatataa acaacagata tgcaatagaa gtaactagga aaagaataaa aaacatgtat
     2821 cttcgtgtga aggacacaga tggcacacta tcagtgagtg ctccttatgg catgagtgat
     2881 acagaaatca ggaaattcgt ggagagcaaa tcagactgga ttgagcggac catgcaggaa
     2941 atgcttgtcg caagacagct taaggaacag gagcctgttt tagcaccttt catggagaga
     3001 gagctgcgca gggagcttaa aatgcagctg cagaggttta tcgacaaatg ggagccggtt
     3061 atgggagtta agtcagccgg ctttacgata aagaagatga agacaaggtg gggatcatgc
     3121 aacgttaagt cacatcatct gaatttcaac cttctgctgg ctaaggtgcc gtcggagtgc
     3181 gcagagtatg tggtggttca cgagcttaca catctgcttg agccaagcca taatgccagg
     3241 ttctggtcgc tgatggaata ttatctgccg gaaagcaaaa agctgcgtaa acaacttgct
     3301 ggcttttctg cgcaggatat gatatgatat tattaaggac tcacttagag aaaggaagct
     3361 tcaaatggaa ttttgggata tatacgataa ggataaaaag ccgacaggcc gtacaatgaa
     3421 aaaaaacgac tggatactta aggatggaga gtatcatctt tcagtgcttg gcgtgattca
     3481 cagaccagat ggcagatttc ttatcacaca gcgtgtcatg acaaaggcat gggcaccggg
     3541 ctggtgggag gtttcaggag gagctgcaca ggcaggtgag agctcattta ccgcagtctg
     3601 cagagaggtg aaagaagagg tgggtcttga tgtatcacag tgtccgggag ggcttgccta
     3661 tacctatcac agagagaatc cgggcgaggg tgacaactat ttcgtggatg tttaccgctt
     3721 tgaacttgat ttcaaggagt ctgacgtaca gattcagaca gaggaggcac ttgggtttaa
     3781 aattgccaca ctcgatgaaa taaaagagct tgctgcgcag ggagtgttcc tgcactatga
     3841 cagcataaag caggt
//
LOCUS       DPUF01000183            2950 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3078, whole
            genome shotgun sequence.
ACCESSION   DPUF01000183 DPUF01000000
VERSION     DPUF01000183.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3078"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="DHV81_06505"
     CDS             complement(<1..182)
                     /locus_tag="DHV81_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCI94114.1"
                     /translation="MTTELIPIYILIFAFGIVIGSFLNVCIYRIPQHETVVTERSHCM
                     RCGYQLAWYDMVPVFS"
     gene            complement(205..681)
                     /locus_tag="DHV81_06510"
     CDS             complement(205..681)
                     /locus_tag="DHV81_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94115.1"
                     /translation="MVKNVFKKLGKKQKNNKGFSLVELIVVIAIMAVLVGVLAPQLIK
                     YVEKSREATDIQTCDNIATALKTYYADEEVAASATATTVTVTLSKTELGTGTTVDTAV
                     KDAGLTKAKIKGTKWTNDTITVVYNKADGTITYTGDSPYYHADKDQFKKGPKTTTP"
     assembly_gap    1531..1592
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1593..2393)
                     /locus_tag="DHV81_06515"
     CDS             complement(<1593..2393)
                     /locus_tag="DHV81_06515"
                     /inference="COORDINATES: protein motif:HMM:PF04754.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI94116.1"
                     /translation="MSAKDSMAKEYFADNARFADLCNNILYGGREVILPENLKERDTT
                     EVLTALGLDKKTIAVQKLRDIFKNASIKYTGKSYVVLIGVENQSDIHYSIPVKNMFYD
                     VMAYGNQVKETAKKHRREKDIATSDEFLSGFTKEDKLIPVITITVYLGTKEWDGPRRL
                     SDMFGDVDEELLPFIPDYRINLLAPREITDFTGFRTSIRQLFEVLQNAYDKEKMQEVL
                     QNDEKFSNVDRETVEAINLFAGTNIDIDEKEEVIDMCKAWEEQKNEGRE"
     gene            complement(2529..2742)
                     /locus_tag="DHV81_06520"
                     /pseudo
     CDS             complement(2529..2742)
                     /locus_tag="DHV81_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          710 a          691 c          536 g          951 t
ORIGIN      
        1 cagctgaaaa ccggcaccat atcgtaccac gcgagctgat acccgcatct catgcagtgg
       61 gacctttcgg tcaccactgt ctcatgctgc ggtatgcggt agatacagac atttaagaag
      121 ctgcctataa ctataccaaa tgcaaatatt aagatgtaga tgggtatcag ttctgtggtc
      181 attgttaaaa agcctcttta tatattatgg tgttgttgtt ttaggaccct ttttgaattg
      241 atctttatct gcatggtaat atggagaatc tccagtatat gtaatagttc catctgcctt
      301 attatataca actgttattg tatcattagt ccactttgtg cctttaattt tcgcttttgt
      361 taatccggca tctttaacag ccgtatcaac agttgttcct gttcctagct ctgtcttact
      421 caatgtaaca gtaaccgtag tagcagtagc cgaagccgca acctcttcgt ctgcataata
      481 tgtctttaaa gctgtagcaa tattatcaca tgtttggata tctgtagcct ctctagactt
      541 ctcaacatac ttgataagct gcggagcaag tacacctaca agcactgcca tgatggcgat
      601 tacaacaata agctctacga gagagaaacc tttgttgttc ttctgtttct ttcctaactt
      661 tttaaataca ttctttacca tagttctttt ctccttttca ttataacatg gtttttgtgc
      721 aaagagctgt gccctatcag catctctttg ttttgtatgc gctcattgta ccataaacca
      781 aagcatattg caagtttttt ctagtacaat aatactattg gtacacaact tttttcgcct
      841 tttttagcaa tttcaaacaa attttaaccg atttattgca tttatttcat atttttcaaa
      901 aaacatctcc caggctgtat ttttgcacag cgtgagaggc ttcactccat gtgaccggct
      961 gaggcataca ctctcgtgag agctacgcta cactgccaag atactgtagg aattatgcct
     1021 tatagatttt ctatgcttga cgaaaccgtc gaaacgcgcc gtccgtggcg cggttctcct
     1081 tgcccattac tgctcacacc agcagtgtga ccagtaacga ttacagccca ttaaaattgc
     1141 ctctacgagg caggggctgt aatctgacaa tagttgacgc attggcgcat agcttgacag
     1201 catggtgtca agcttgcgtt tgaacagcaa cgatatatta catcccagtg gcagttcatt
     1261 tctgtgtatc gtgtgcataa ttcaacagta tctaagcagc ttcgctattc gctcacccga
     1321 gagtatattc gccagccgtt cgtagagaat gaagccccat cctactgtca gcaaaagagg
     1381 aagcaacctc cgggctctcg cccaaagatt gcttcctcta atttacattt cactacaata
     1441 ttagctcaca agccattttt ttacagtttc ctcatcaaaa tccacacatt cagctatatc
     1501 ttctattgag tgaccttttt tctgaagttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttctctgc cttcattttt ctgttcttcc
     1621 cacgccttgc acatatcaat cacctcttct ttctcatcta tgtctatatt cgttcctgca
     1681 aacaggttga ttgcctcgac tgtctctctg tccacattgc taaacttctc gtcattctgc
     1741 agcacttcct gcattttttc cttatcataa gcattctgta atacctcgaa aagctgcctg
     1801 attgaggttc tgaatcctgt aaagtcggtt atctccctcg gagccagcag gttaatccta
     1861 taatccggta tgaatggcag aagctcctcg tccacatcac caaacatatc tgacagcctc
     1921 cttggtccat cccattcctt tgtcccgaga tacactgtaa tcgtaattac tggaatcaac
     1981 ttatcctctt tcgtaaatcc cgagagaaat tcatcagaag ttgcgatatc cttttccctg
     2041 cggtgctttt tggctgtttc ctttacctga ttgccatatg ccatgacatc ataaaacatg
     2101 ttttttaccg gaatggaata gtgtatatcc gactgatttt ctactcctat cagtaccaca
     2161 tatgatttgc cggtatattt gatgctggca tttttgaaaa tatccctcag cttctgcacc
     2221 gctatggtct ttttgtcaag ccccagtgcg gtcagcacct ctgtcgtgtc cctttcctta
     2281 aggttttccg gcaggatgac ctctcttcct ccatacagta tattgttgca caggtcagca
     2341 aacctggcat tgtcagcaaa gtattccttt gccattgagt cttttgcgct caatccttct
     2401 cctcctgttc tcttgtgtga gcaggaaaat ttggtgccaa tgagggtata gtggtacagt
     2461 ttacaaggta tacctgtgct gttcttcttt aatatttacg cacggtatac agttcatccg
     2521 ccttttgact acattttctg cattctgtct acatacggca tttcaatttt cctgctgttg
     2581 attggtaaat aaagcattga aaacttccat gtcctttgta gaataatgcg aagtggtctg
     2641 ccctcttcaa agtggtgcgc acctgaaaaa ctgttatgtg gtgccgctta cagaaatata
     2701 atgctttgag ttgattgtag catagaggtg gtgttttgtc atcacatttt ttcttttttg
     2761 ccacagcgta aggggcttta tcccatgtga ccggctgggc acactctccc gcgtgagcga
     2821 atagcgaagc tgcctagata ctgttgaatt atgcacacga tacacagaaa tgccctgcca
     2881 cggatggcag ggcaaggaga accgcgccac ggacggcgcg tttcgacggt ttcgtcaagc
     2941 atagaaaatc
//
LOCUS       DPUF01000184           10646 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_224, whole
            genome shotgun sequence.
ACCESSION   DPUF01000184 DPUF01000000
VERSION     DPUF01000184.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_224"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="DHV81_06525"
     CDS             <1..450
                     /locus_tag="DHV81_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94117.1"
                     /translation="IWTVINVIMYYAAMILVLAAVTCAIGKWSTKPDDMLMEIGIDMQ
                     QFATGNEVLVWLILPMLCACTIAVVQLTISIFAGAIAGYIVSIVYLVVSVYWVSPFLM
                     GNYLMIIRNNRICAGGMDAAAGIISCIIVMVVSVVLGSVYFNRKNIL"
     gene            459..1085
                     /locus_tag="DHV81_06530"
     CDS             459..1085
                     /locus_tag="DHV81_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HCI94118.1"
                     /translation="MKIEIEHVSKKIKDALVLDDVCMTLESGNIYGFQGVNGSGKTML
                     MRAVSGLMYPTSGTISIDGKVLGKNMAFPEKIGMLIENPAFIDSYTGYDNLKMLASIN
                     KGEVDISRALETVGLNPQDKRKYRKYSLGMKQRLGIACAIMEEPKLLLLDEPFNALDK
                     EGQEKLSEIIRDMRDKGSLILLSSHDKDELENLSDVIYLVDSGRFKLK"
     gene            1101..1685
                     /locus_tag="DHV81_06535"
     CDS             1101..1685
                     /locus_tag="DHV81_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94119.1"
                     /translation="MKQRKSKQVRLFSGMLIGIVMAAVLARIVYLNHCYPSPKVITYT
                     ENDTIKLGNYEIKQTGWEWGDGSLLKEKCPDYVYHQMDDGSEYPFDSVRVGFITLSVT
                     KVKDDTTSLDLTSLAFEMGTNGNQYDMELFFKLNPTLEALEIKLNAGEETSFVIPYTM
                     NEQQVSKKDWNRVDKMKLYMVLQYYPQKIRLCCN"
     gene            1985..2575
                     /locus_tag="DHV81_06540"
                     /pseudo
     CDS             1985..2575
                     /locus_tag="DHV81_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3276..4094
                     /locus_tag="DHV81_06545"
     CDS             3276..4094
                     /locus_tag="DHV81_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94120.1"
                     /translation="MIRAEMKKYLYFPYLLLAVIGCVVLAFSANADYDPSGSAVSVFR
                     LAITGIAHKSEQPIEYSALFMWIKGMNGWLPLILPLLMSFGYIAVLSAERHNGMTGFL
                     LIRSQNAKYCATKVTAGVLTGGILFITANIVFGLMMLIAFPAYISFSVNEQMMYADWY
                     GTGSMVLIYVVKRLIGSFLYGMAASMFGIGAAIFFRDRYMLLCLPFLLNYIYTQILQR
                     LTLENPAVERFNSSNLMNVSGESGWVWTVGLTIVCYVILVLLYYAQVKRRHCDG"
     gene            4087..4941
                     /locus_tag="DHV81_06550"
     CDS             4087..4941
                     /locus_tag="DHV81_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94121.1"
                     /translation="MDRFSFRAAVHIAQAEFRQWLRSSRIIILFVMLVFVNVQVIGPL
                     RQCKMLMGEMLSIPEAFVALGNSGVILLIIPILFLVLMADFPQRTGIDRFYQMRCTKR
                     TWIFGQAVFAAAASCFVLLFLLVSSCVLMIGTGRWSLSFSDAVTHFTSVYPDRTGDYV
                     VQLLPGNLYQQMTLEQALIHTFCLLLLYFLLISYVLLFSAVSNHRYVGILVNGFAIIL
                     GAVTCEVRMKQMWILPMAHTIPWLHYDEYLDRMNFPMWGSYLYLGAISALLLVLCFAR
                     AGHYQVKE"
     gene            4953..5585
                     /locus_tag="DHV81_06555"
     CDS             4953..5585
                     /locus_tag="DHV81_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI94122.1"
                     /translation="MIEIRNVSLRLDGKSILNNISLTLEPGKIYGLVGSNGSGKTMLM
                     KCICGFVHPTEGEIKTDGRRVGKDVDYLPNAGIIIETPGFIPYYSGLQNLRLLAGIRR
                     KVTDDQIRKALKITGLDPDSRLWVRKYSLGMRQRLGIAQAIMEQPDILILDEPMNGLD
                     KHGVADIRELLMELKKQGKLILLASHNPADIELLCDEVYEMELGNIRNFS"
     gene            5716..6189
                     /locus_tag="DHV81_06560"
     CDS             5716..6189
                     /locus_tag="DHV81_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCI94123.1"
                     /translation="MLLTTTNNNLFDTFFSDPWFNDPWYDDRDVQKAQKKLYGHNGKR
                     MMLTDIKESDKGYELEMDLPGFKKDEIKASLENGYLTITAEKGLEKDQKEEEGKKYIC
                     RERYSGSCQRTFYVGDEIEQDDIKASFKHGILRLDIPKKQPKPQVEEKKCISIEG"
     gene            6311..7366
                     /locus_tag="DHV81_06565"
     CDS             6311..7366
                     /locus_tag="DHV81_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCI94124.1"
                     /translation="MAAKRDYYETLGVNKSADKEAIKRAYRKLAKKYHPDTNAGNPHA
                     EEMFKDVTEAYNVLSDEKKRKLYDEFGFAGLQEGFSEEAARQAAQGGFGGFGGFGGNG
                     SFSGSYSSNGGPFTHQEFHFENGSGDMDDIFSMFGDMFSHGGGNHSGWSTSGNSGFTG
                     NSGSWSNFSGGRSSAGRKGSDVMADLTISFDEAVFGCEKSISLQDPTTGKVSNLSIHI
                     PAGIESGKTVRLKGQGNPGRNGGAAGDVLLNVNVTPSSEFERKGQNVYSTIRVPFTTA
                     ALGGKARVHTLYGDVECSIKAGTQGGSKIRLKGKGISSMKNPSVKGDQYVTVEISVPR
                     RLTPRAKEALEEYRAVS"
     gene            7474..9060
                     /locus_tag="DHV81_06570"
     CDS             7474..9060
                     /locus_tag="DHV81_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI94125.1"
                     /translation="MGRFLNPDNSSFQDVINSEIYVDKTGLLDYTNSVINTTSKFICN
                     SRPRRFGKSITADMMAAYYSMGCDSRELFMGYDISKKGSFEKYLNKYDVLHLDIQWCI
                     EPAKGPENVVSYIIENTVAELKLAFPDVKVEGRVTLSDMLSQINAQTGKRFIVIIDEW
                     DVLVRDEANNQSVQEEYINFLRGLFKGSSATRYIALAYLTGILPIKKLKTQSALNNFE
                     EFTMISPGGFAHYIGFTEDEVKKLCEKSGRDFELVKKWYDGYLLGEYHIYNPKAVSNV
                     IKWGNFQSYWSQTGTYDSIQPLINKDFDGLKSAIVEMISGAETEVKTTTFQNDMVTFR
                     NKNDVMTLLIHLGYLAYDQKRQMAYIPNEEIRMEFMNAVEDDKWNELIEFETQSERLL
                     EATLDMEEKVVAEYIDKIHNEYASTIQYHNENSLSSVLAIGYISAMKYYFKPVREMPT
                     GRGFADFVFIPKQEYVMDYPALVVELKWNKDVKTAINQIKDRQYPQSVLQYTGNILLV
                     GINYDKKTKVHECIIEELVK"
     gene            9074..9538
                     /locus_tag="DHV81_06575"
     CDS             9074..9538
                     /locus_tag="DHV81_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCI94126.1"
                     /translation="MSNYIMDLRKVVGHAPLLQAAASIIIENENGQVLLGKRTDNHQW
                     GYAGGSIELGETVEEAAKRELFEEMGLVADEMELFYINSGEETHYIYPNGDEVYNVEI
                     IYICRKYHGTIKRQEDEVEELKFFDVDDIPEDISDPIRPVFREYIKMRKRTK"
     gene            9628..10497
                     /gene="aroE"
                     /locus_tag="DHV81_06580"
     CDS             9628..10497
                     /gene="aroE"
                     /locus_tag="DHV81_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCI94127.1"
                     /translation="MEKRISGHTGLLALIGSPVGHSGSPAMYNYSFEKLGLDYAYVAF
                     DIKEDKVKDAIAAMKTFNMRGCNVTMPDKVEAAKYMDELSPAAQIIGAINTIVNDNGK
                     LTGHITDGEGFVHNLKDHGIDIKGKKITVAGGGGAATAIQVQCALDGAREITIFNKND
                     AFFERTLQTAEKIKKAVPDCVVNVYDIDDTAKMTEEIQSSDIFANATIVGMKPLDDQS
                     VVKDLSAFRAGLVVADAVYNPEETKLLREAKEAGCTCVGGKGMLLWQGVAAFKLYTGQ
                     DMPVDDVKKLFFS"
BASE COUNT         3326 a         1726 c         2685 g         2909 t
ORIGIN      
        1 atatggacgg tcataaatgt gattatgtat tatgctgcaa tgatactggt tcttgcggct
       61 gtgacatgtg caatcgggaa gtggagcaca aagcctgatg atatgctcat ggaaattgga
      121 atagatatgc agcagtttgc gaccggaaat gaagtgcttg tatggctgat actgccaatg
      181 ttatgcgcct gtactattgc ggtggtgcag cttactatca gtatttttgc cggtgcgatc
      241 gctggatata ttgtttccat agtttatctt gtggtatctg tttactgggt gagtccgttt
      301 cttatgggca attatcttat gatcatcaga aacaacagaa tctgtgcggg cggaatggat
      361 gcggctgccg gaataataag ctgcattatt gtgatggtgg tttctgttgt gctcggaagt
      421 gtgtatttta atagaaaaaa tattttgtga ggtattttat gaagatagag atagaacatg
      481 tttctaaaaa gataaaggat gctctggtac tcgatgatgt atgcatgaca ctggaaagtg
      541 gtaatatata tggatttcag ggcgtaaacg gttcaggcaa gacaatgctg atgagagcgg
      601 taagcggact tatgtatcct acaagcggta ccatcagtat tgacggcaag gtgcttggaa
      661 aaaatatggc ttttccggaa aagataggta tgcttattga gaatcctgca tttatagata
      721 gttatacagg ctatgacaat ctaaaaatgc ttgcatcaat aaataaaggt gaagttgata
      781 taagcagggc acttgaaaca gttggtctga acccgcagga taagagaaag tatagaaaat
      841 attctcttgg aatgaagcag agactgggta ttgcctgtgc cattatggag gaaccaaagc
      901 ttcttttgct ggatgaacct tttaacgccc tggacaagga gggacaggaa aaactgtcag
      961 aaatcataag ggatatgcgt gataaaggaa gtcttatact gcttagctct cacgataagg
     1021 atgagcttga aaatctatca gatgtaatct atctggtgga cagcggaagg tttaaattga
     1081 agtaacgagg agaatttatt atgaagcaaa ggaaatcaaa acaagtaaga cttttttcgg
     1141 gaatgctcat aggaattgtg atggctgctg ttttggcaag gattgtttat ctgaatcatt
     1201 gctatccatc cccaaaggtc atcacatata cagaaaatga cactattaag cttggaaatt
     1261 atgagataaa gcagaccgga tgggagtggg gcgatggcag tctcctaaaa gaaaaatgtc
     1321 cggattatgt atatcatcag atggatgatg gttcagagta tcctttcgac agtgtgcgtg
     1381 tgggatttat aacgttgtca gttaccaaag taaaggacga tacaacatca cttgacctta
     1441 caagccttgc atttgaaatg ggaaccaatg gtaatcagta tgacatggaa ctgtttttta
     1501 aattaaatcc tactttggaa gctttggaga taaagcttaa tgccggtgag gagacgtcct
     1561 ttgtgatacc gtatacaatg aatgagcagc aggtatcaaa gaaagactgg aacagggtag
     1621 ataagatgaa gctgtatatg gtgctgcagt actatccgca gaagataagg ttgtgctgta
     1681 actgattttc aatttttaat tgatctatat aaaatgtacc aaaagtgaat aaaaaatgtc
     1741 atctcatgta aattgtattg aaaaaaatgg gaaaatgaat ataattgaaa ttgtaatcga
     1801 tttctaataa caataattat tgttattaag taacttaaaa catagtatag taaaggagaa
     1861 gtctatgggg aaaagatgca atgcaaaagg tacgatttat aaagtagtat aaaaaagata
     1921 aaaatatgtt agccggtatg tttcccatac cggctaattg tgttacaaaa tgggggaatt
     1981 atatatgaac aaacatcaca aaacagtact aataccagcc ctttgcattg cagcattttt
     2041 taccatgtct gcatgtgcag acagcaaaat ggcagatacg cagcagaaga atacagcgga
     2101 taaatcagat gcgcagcagg ggattgatat agctgcgtat gagaaaaagc ttgccaataa
     2161 cagggagaat tacaagataa gctgcgagca gtacagcaca gataccgata cggtaaaaaa
     2221 cactgattac aggaatttat cgtttacaaa ctgtactttt aaagatatgc cggaaacgaa
     2281 tgagttgtat gtcatggagg gggaagtaca cggcattact acacaggaga gctgggatac
     2341 aataaaggac tggcttgaat caatcggtaa gctcgataca actgatatgg attcggaggt
     2401 aaggatcata aacattgacc ttggaatgga tgatacaaag gaaacaccat acagttatcc
     2461 ggctcttaag gatcatatgg atcttgcatc aggggcagga gcacttgtaa atacgaatga
     2521 gtgtcatata cagatagcag aaaatggcat atattccatg agtgatggaa agataggagc
     2581 atatctgaat acggatgcgc atgcgagcag tgacgctttg gggacataca cagatgatgt
     2641 tgtaaaatcg gggacaatag acgagcttgg aaaagaaagc tatcagctta taaatggtga
     2701 attgaccatt gaacaggggg cagagattac aggggaatat tttgaaaaag gtactccgtt
     2761 tgtacccaca gatggcatta cattgtcagt tccggaggtt cgcgtgttca ggctcggaca
     2821 ggtgtacgga tatgattata tggttcaaag gcagtaccat ggtgttccaa tagcatatca
     2881 tgattatgga aattatcaga tatatggaaa tgagtataat tttgaagagg atatcaaaca
     2941 tgtatatgta gtggattctg acggagtcag tgcttattcg ggagttaacg attcggaacc
     3001 gttaaaaaat ttgtattcgt cggatgagat attgggaata aaggatgccg cagatatttt
     3061 aagcgggaag atggcgaagt ttttaaatgt tgatgtacaa agtgcatgtc ttacatatgt
     3121 accgcttaat tttggagata gttctgagtg taaaatatat cttccggcat ggcagtttga
     3181 cggaataaac agaactaagg gcgaaaatat caggatgtat cttgatgcat ttacaggaga
     3241 ggtctattac tatacctatg gggaggcggc ttagtatgat aagggcggaa atgaaaaaat
     3301 atctgtattt tccgtatctg cttcttgcgg tcataggatg tgttgtactt gcttttagcg
     3361 caaatgcgga ttatgatccg tcaggcagtg ccgtgtcggt attccggctt gcaataaccg
     3421 gcattgcaca taaaagtgag cagccgatag aatacagcgc actttttatg tggataaaag
     3481 ggatgaacgg ctggcttccg cttatactgc cgcttttaat gtcatttgga tatatcgcag
     3541 tactttccgc ggaacggcac aacggcatga caggtttttt gctcataagg tcgcaaaatg
     3601 caaagtattg tgccacaaag gtgacagcag gagtgcttac aggtggaatt cttttcatca
     3661 cagcgaacat tgttttcgga cttatgatgc ttattgcatt tccggcatat atatcatttt
     3721 ccgtgaatga gcagatgatg tatgcagact ggtacggaac aggcagtatg gtgctcatat
     3781 atgtggtaaa acggcttata ggttctttct tatatggtat ggcggcaagt atgtttggaa
     3841 taggagcagc aatatttttt agggatcggt atatgctgct gtgccttccg tttctactta
     3901 attacatata cacgcagatt ctgcagagac ttacacttga aaacccggca gtggagaggt
     3961 ttaactcatc aaatctgatg aatgtgtcgg gtgagagcgg ctgggtatgg acggtaggac
     4021 ttactatagt ttgttatgtg atacttgttc tgctgtatta tgcacaggtg aaaaggaggc
     4081 attgcgatgg atagattttc gtttcgggca gcagtgcaca ttgcgcaagc tgaattcagg
     4141 cagtggctgc gcagcagcag gataatcata ctgtttgtca tgctcgtatt tgtgaatgtg
     4201 caggtgatcg ggccgcttcg acagtgcaaa atgcttatgg gagaaatgct ttctatacct
     4261 gaggcttttg tggctttggg aaattcggga gttatccttc ttataatacc tatacttttt
     4321 cttgtgctga tggctgattt tccacagagg acgggaattg acaggtttta tcagatgcgc
     4381 tgtacaaaga ggacatggat atttggacag gcagtttttg cggcagcggc atcctgtttt
     4441 gtgcttttat tccttttggt gtcatcgtgc gttcttatga tagggacagg cagatggagt
     4501 ctttcatttt ctgatgcggt cacacatttt acatcagttt atccggatag gacgggagat
     4561 tatgtggtgc agcttcttcc tggaaatctg tatcagcaga tgacacttga acaggcactt
     4621 atacacacat tctgtctgct gcttttatat tttctgctca tatcatatgt gctgcttttt
     4681 tcggctgtca gcaatcacag atatgtgggg atactcgtaa atggctttgc aatcatactc
     4741 ggagcagtca cctgtgaggt cagaatgaag cagatgtgga tacttccgat ggcgcatacg
     4801 ataccgtggc tgcactatga tgaatatctt gacaggatga attttccgat gtggggatca
     4861 tatctttatc tgggagccat aagtgcgctg cttcttgtgc tttgctttgc aagggcagga
     4921 cattatcagg taaaggaata gggggaccga gcatgataga aataagaaat gtatcgctaa
     4981 gactggacgg caaatcaatt ttaaataata tttcgctgac acttgaaccg gggaaaatct
     5041 atggacttgt tggcagcaac ggaagcggca aaacaatgct gatgaaatgc atctgcggat
     5101 ttgtgcatcc gacagaggga gagataaaaa cggacggcag gcgtgtggga aaagatgtgg
     5161 attatctgcc aaatgcagga ataatcattg aaacaccggg atttataccg tattacagcg
     5221 ggctgcaaaa tctacggctg cttgccggaa tacgcagaaa ggttacggat gatcagatac
     5281 gtaaggctct taagataaca ggacttgatc cggacagcag actttgggtg cgcaagtaca
     5341 gtcttggaat gaggcagcgc cttgggattg cacaggcaat catggagcag ccggacatac
     5401 tgatactgga tgagccgatg aacggactcg ataaacacgg tgtggctgat atacgtgagc
     5461 ttttgatgga gcttaaaaag cagggaaagc tcatacttct ggcaagccac aatcccgcag
     5521 atatcgagct gctttgtgat gaagtatatg aaatggagct gggaaacata agaaatttca
     5581 gttaataaaa aatttataat tttttattag cactcgctat tgacgagtgc taataatggt
     5641 gctataacat acttgtaaac aaaagaaagc aaatttccaa aaggaaagtt acaggtatat
     5701 aaggaggtat gacctatgtt attaacaact acaaacaaca acttatttga tacatttttc
     5761 agtgatccat ggttcaatga tccttggtat gatgacaggg acgtacagaa ggcacagaag
     5821 aaattatacg gacacaacgg aaagagaatg atgctcaccg atatcaagga aagcgataaa
     5881 ggctatgagc ttgagatgga tcttccggga ttcaaaaagg atgagattaa ggcttcttta
     5941 gagaatggtt atctgaccat tacagcagag aaaggcttag agaaagatca gaaagaggaa
     6001 gagggcaaga agtatatctg cagagagcgc tactcaggaa gctgtcagag aacattctat
     6061 gtgggtgacg agattgagca ggatgacata aaggcatcct tcaagcatgg aatccttcgc
     6121 ctggatattc caaagaagca gcctaaacca caggttgagg aaaagaaatg tatctccatt
     6181 gagggataat cagatattgc aaatgataag cgggctgcct gtgcagtccg cttatactat
     6241 atggtgtaat gtatattgtt taattatgat gataaattgt tacaggtaaa tagaaaggca
     6301 ggtgagtata atggcagcaa agagagatta ttatgagact ctcggagtga acaagagtgc
     6361 ggataaagag gcaataaaga gggcttatcg taagctggca aagaagtatc atcctgatac
     6421 aaatgcaggc aatccgcacg ctgaggagat gttcaaggat gtcacggagg catacaatgt
     6481 cctcagtgat gagaaaaaac gtaagctcta tgatgagttt ggttttgcag gactgcagga
     6541 gggcttttcc gaggaggctg caagacaggc tgcacagggt ggcttcggtg gatttggtgg
     6601 cttcggcggc aatggctctt tcagtggctc atacagctca aatggcggac catttacaca
     6661 tcaggaattt cattttgaga acggctccgg ggatatggat gatattttct caatgttcgg
     6721 agatatgttt tcacatggtg gcggtaatca cagtggctgg agtacaagtg gtaacagtgg
     6781 atttaccgga aatagcggta gctggagtaa tttctcaggt ggcagaagtt cagccggtcg
     6841 caagggttct gatgttatgg ctgaccttac gatttcattt gacgaggctg tttttggatg
     6901 cgagaaatct atttcgttac aggatccgac cacaggcaag gtttccaatc tgtccatcca
     6961 tattccggca ggaatcgagt ctggcaagac agtcagactt aaaggacagg gcaatccggg
     7021 cagaaacggt ggtgcagccg gagatgtgct tctcaacgta aatgtgacac cgagcagtga
     7081 gtttgagcgg aagggacaga atgtatacag tacaatccgt gttccgttta cgacagcggc
     7141 tctcggtggt aaggcgaggg tgcatactct ttatggagat gtggagtgca gcatcaaggc
     7201 aggtacacag ggcggcagca agatacgtct caagggcaag ggtatttcga gcatgaagaa
     7261 tccttcagta aagggagacc agtatgtgac tgttgagatt tcggttccac gcaggcttac
     7321 gccaagggca aaggaagcgc ttgaggaata cagggctgtg agctgatgaa ttttatgtgc
     7381 tttatatatg gaaaacatat ttgaaatccg ctatactata ggtattatag tacagcggat
     7441 tttttgcttg atgagatttt aaaaggagag actatgggaa gatttttaaa tccggataac
     7501 agttcattcc aggatgtcat taactctgag atatatgttg ataaaacagg tttgctggat
     7561 tacacaaaca gtgtgataaa taccacatca aaatttatat gtaacagcag accgcgcaga
     7621 ttcggtaaat ctattacagc agatatgatg gctgcatatt atagcatggg ctgtgattcg
     7681 agagaactgt ttatgggata tgatataagt aagaagggta gctttgagaa atatcttaat
     7741 aaatatgatg tgctgcatct ggatatacag tggtgtatag aaccggcaaa aggtccggaa
     7801 aatgttgtat catatattat agagaataca gtcgctgagc ttaaacttgc atttccggat
     7861 gtgaaggttg aagggagagt tacgctttca gatatgcttt cacaaataaa tgcacagaca
     7921 ggaaaaagat tcatagtgat aatagatgag tgggatgttc tagttcgcga tgaggcaaat
     7981 aatcagtcgg tacaggagga gtatattaat tttctcagag gactttttaa aggttcatcg
     8041 gctacaagat atattgcgct tgcatatctg accggaatct tgccgattaa gaagcttaag
     8101 acacagtcgg cacttaataa ttttgaagaa ttcactatga tatctcctgg gggatttgcg
     8161 cattatattg gttttacaga ggatgaagtg aagaaattat gcgaaaagtc cggacgtgat
     8221 tttgaacttg tgaagaaatg gtatgatggt tacctgctgg gtgaatatca tatatataat
     8281 ccaaaagctg tgagtaatgt aattaaatgg gggaattttc aaagctattg gagccagaca
     8341 ggcacatatg actctatcca gccacttata aataaggatt ttgatggtct taagagtgct
     8401 atagttgaaa tgatttcagg tgcagaaaca gaggttaaaa ctactacatt tcagaacgac
     8461 atggttacat tcagaaataa aaatgatgtt atgacgctgc ttatacacct tggatatctg
     8521 gcatatgatc agaaacggca gatggcatat attccgaatg aggaaattcg tatggagttt
     8581 atgaatgcgg ttgaggatga taagtggaac gagctgatag agtttgaaac ccagtcagaa
     8641 cggcttcttg aggcaacgct tgatatggaa gaaaaagtgg ttgcggaata tattgataaa
     8701 attcacaacg agtatgcttc cactatacag taccataatg aaaattcatt gagtagtgtg
     8761 cttgccatag gatatatttc ggccatgaaa tattatttta agcctgtacg tgaaatgcca
     8821 acaggcagag gctttgctga ttttgtattc attcctaaac aggaatatgt catggattat
     8881 ccggctttgg ttgttgaact taaatggaat aaagacgtaa aaactgctat aaaccaaata
     8941 aaagacagac agtatccgca gtctgtttta cagtataccg gaaatatttt acttgtcggt
     9001 attaattatg ataaaaagac taaagtgcat gagtgtataa tagaagaact ggtaaaataa
     9061 gaaaggatac aaaatgagca attatataat ggatcttaga aaggtggtcg gacatgcccc
     9121 tttactgcag gcggcagcga gcatcatcat tgaaaatgaa aacggacagg tgcttctcgg
     9181 aaagcgcaca gacaatcacc agtggggcta cgcaggcggt tctatagagc ttggagagac
     9241 tgttgaggag gctgcaaaaa gagaactctt tgaggagatg ggacttgttg cagacgagat
     9301 ggagctgttt tatatcaatt caggcgagga gactcactac atctatccta acggcgatga
     9361 agtttataat gtggaaatca tatacatatg ccgcaaatat catggcacca taaagcgcca
     9421 ggaggatgaa gtcgaggagc ttaagttttt cgatgtggat gatattccgg aggatatttc
     9481 tgatccgatc agacctgtat ttcgtgaata tataaagatg aggaaaagaa caaaataaac
     9541 tttaacgctt taaatgaaaa aagtgcttgc gcatgtctta taaaagagtt atcataatac
     9601 aaaacgaaac acttaggagg atttacaatg gaaaagcgta tttcaggtca cacaggttta
     9661 ttagcactca taggatcacc ggttggtcat tcgggttcac cggctatgta caattacagt
     9721 tttgaaaaac tcggtctgga ctatgcatac gttgcatttg atataaaaga agacaaggtt
     9781 aaggatgcta ttgcagcaat gaagaccttc aacatgcgtg gatgtaatgt gaccatgcct
     9841 gataaggtag aggctgcaaa gtacatggat gagctttcac cggctgccca gattatcgga
     9901 gccataaaca ctatagtaaa tgacaacggc aagcttacag gacacatcac agatggtgag
     9961 ggattcgtac acaacttaaa ggatcacgga attgacatca aaggaaagaa aatcacagtt
    10021 gccggaggcg gcggagccgc tacagctatc caggtgcagt gcgcattgga cggagctcgt
    10081 gagattacta ttttcaacaa aaatgatgct ttcttcgagc gtacactaca gactgcagag
    10141 aagatcaaaa aagcagttcc tgactgtgtt gtaaatgtat acgatattga tgatacagcc
    10201 aagatgacag aagaaatcca gtcaagcgat atctttgcaa atgcaacaat cgttggcatg
    10261 aagcctctgg atgatcagag cgtggtaaaa gatctttcag ctttcagagc ggggcttgta
    10321 gttgcagatg cagtttacaa tccggaggag acaaagcttc ttcgcgaagc aaaagaggca
    10381 ggctgcacat gcgtaggcgg aaagggaatg ctcttatggc agggcgtcgc cgcattcaag
    10441 ctttacacag gacaggatat gccggttgat gatgtgaaga aactgttctt cagttagatt
    10501 taagttctta ttacaatatc attagatagc ttcctgttat acaggaatat gataaaacaa
    10561 cagctcgcgg ttttccgcga gctgttgttt tttaggagtt gtgcgcctag cggcgcacgt
    10621 ttctaacggg ttaaagtccc gaatcc
//
LOCUS       DPUF01000185            5276 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16225, whole
            genome shotgun sequence.
ACCESSION   DPUF01000185 DPUF01000000
VERSION     DPUF01000185.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16225"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="DHV81_06585"
     CDS             complement(<1..293)
                     /locus_tag="DHV81_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination regulator"
                     /protein_id="HCI94128.1"
                     /translation="MNNIVIAFPKKEVANSIKKILAQSGYTVTAVANTGASALSSMNG
                     LNMGIIICGYRFSDMMYSEIYEYMPKEFQMLLIASAAGIMEKNVDNLMSLSMPL"
     gene            complement(309..1640)
                     /gene="glnA"
                     /locus_tag="DHV81_06590"
     CDS             complement(309..1640)
                     /gene="glnA"
                     /locus_tag="DHV81_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCI94129.1"
                     /translation="MAKYSKNDIIRIVDEEDIEFIRLQFTDIFGTLKNVAITASQLEK
                     ALDNKCMFDGSSIEGFVRIEESDMYLHPDLDTFMIFPWRPQQGKVARIICDVYMADGT
                     PFEGDPRYILKKQIEKAKQMGYIFNVGPECEFFLFHLDENGQPTTISHERAGYFDLGP
                     NDLGENARRDMVLTLEDMDFEIEASHHEVAPAQHEIDFKYEEALHTADNIMTFKLAIK
                     TIAKRHGLFASFMPKPKHGVSGSGMHINMSLSKDGVNIFDNKEDKLGLSEEAYYFIGG
                     IMKHMKGMTVITNPIVNSYKRFVPGYEAPIYITWSATNRSPLIRIPAARGEGRRVELR
                     NPDPSANPYLVLALCLAAGLDGIKNKIQPDAELRENVFELTEQDKKRLGIESLPADLH
                     EAVECLKSDDFIREVLGEHIYDKYIEAKEAEWNDYRAAVTQWEIDEYLYKF"
     gene            complement(1642..2427)
                     /gene="hisA"
                     /locus_tag="DHV81_06595"
     CDS             complement(1642..2427)
                     /gene="hisA"
                     /locus_tag="DHV81_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HCI94130.1"
                     /translation="MEFRPCIDIHNGKVKQIVGGSLKDRGDFASENFVSEQDSRFYAD
                     MYRKSGIKGGHIILLNSVDSEYYEDTKEQAVMALEAYPQGLQVGGGITADNAMEFIDA
                     GAAAVIVTSYVFKDGRINYANLNRLKDTVGSDRLVLDLSCRKKDGQYYIVTDRWQNFT
                     DEAVTTELLDKLSSYCCEFLVHAVDVEGRVNGIEKELVAMLGSWGRIPVTYAGGVSSF
                     DDLKCIRQLGQNHLNVTIGSALDLFGGEMEYDKVIAFCNNKEI"
     gene            2711..4168
                     /locus_tag="DHV81_06600"
     CDS             2711..4168
                     /locus_tag="DHV81_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCI94131.1"
                     /translation="MGTIIGEGITFDDVLLVPQYSEVTPNMIELQTHLTKKIVLNIPM
                     MSAAMDTVTESKMAIAMARQGGIGIIHKNMSIEAQADEVDKVKRSENGVITDPFYLSP
                     DHTLQDADNLMAKFKISGVPITKDGKLVGIITNRDLKFETDFTKKISESMTSENLITA
                     PEGITLDEAKKILAKARKEKLPIVDKDYNLKGLITIKDIEKQIKYPLSAKDDQGRLLC
                     GAGVGITGNMMERVDALVKSHVDVIVVDSAHGHSKNILEAVKKIKAAYPDLQIIAGNI
                     ATGAAAKALIEAGADAVKVGIGPGSICTTRVVAGIGVPQITAIMDCYKVAKEYGVPVI
                     ADGGIKYSGDMTKALAAGANVCMMGSMFAGCDEAPGTFELYQGRKYKVYRGMGSIAAM
                     ENGSKDRYFQEGAKKLVPEGVEGRVAYKGTLEDTVFQLVGGIRSGMGYCGCKTIEDLK
                     ENGQFVKITAASLKESHPHDIHITKEAPNYSTDDK"
     gene            4204..>5276
                     /locus_tag="DHV81_06605"
     CDS             4204..>5276
                     /locus_tag="DHV81_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HCI94132.1"
                     /translation="MDADDISQIIFLLILLALSAFFSSSETALTTVNKIRMRTLAEAG
                     NTKAKKVLKVTENSPKMLSAILIGNNIVNLSASSLTTSLAIKLFGNVGAGVATGILTF
                     LILIFGEVSPKTLATIKADKISLSIAGFISVLMVVLTPVIFIINKLSLGVIFLFGIRQ
                     SDAKRVMTEEELRTIVDVGQEDGVIEDEERDMIHNVFDFGDAEAKEVMVPRIDMTFVH
                     VDSTYDDLISIFREDKFTRLPVYDESTDNVIGIVNMKDLLLLKDEDKEHFSIRNIMRE
                     PYFTYEHKNTANLFLEMRESSISLAIVLDEYGVTAGLITLEDLLEEIVGEIRDEYDSD
                     EQDDITRINDYEYIVLGSANLEDV"
BASE COUNT         1463 a         1234 c         1075 g         1504 t
ORIGIN      
        1 agtggcatgg ataatgacat cagattatcc acattttttt ccatgatacc cgctgccgac
       61 gctatgagaa gcatctgaaa ctccttaggc atatactcgt atatctcact gtacatcatg
      121 tctgaaaatc tgtatccgca tatgatgatt cccatattca gtccgttcat gctcgaaagt
      181 gctgaagctc ctgtatttgc aaccgccgtg actgtataac cgctctgcgc taatattttt
      241 ttgattgaat tggctacttc ctttttagga aatgctataa caatattatt cacggttcgt
      301 tacatccttt aaaacttgta cagatattca tctatttccc attgggtaac cgcagctctg
      361 taatcgttcc actctgcctc ttttgcctct atatacttgt cgtagatatg ctctccgagt
      421 acctcgcgga taaaatcatc tgacttaagg cactcaaccg cctcatgtaa atcagccgga
      481 agtgattcaa ttccgagtct ctttttatcc tgttctgtga gctcaaatac attctcacga
      541 agctctgcat ccggctgtat cttgttcttt atgccgtcaa gtcctgctgc caggcacagt
      601 gccagaacca gatacggatt tgctgaagga tcagggtttc taagctccac tctccttccc
      661 tcacctcttg ctgcagggat tctgataagc ggacttctgt ttgtagctga ccaggtgata
      721 taaatcggtg cctcatagcc cggcacaaag cgcttatatg aattgacaat cggattggtg
      781 atgactgtca tgcccttcat atgcttcatg ataccgccta tgaagtagta tgcctcctcg
      841 ctgagtccaa gcttatcctc cttattgtca aatatattga caccatcctt tgaaagtgac
      901 atattgatat gcattcccga gccgctcaca ccatgctttg gctttggcat gaagctggca
      961 aaaagtccgt gccgctttgc gattgtcttt atagcaagct taaaggtcat gatattgtcg
     1021 gcggtatgaa gtgcctcctc atacttgaaa tcaatctcat gctgtgcagg tgccacctca
     1081 tgatgtgatg cctctatctc aaaatccata tcctcaagag ttagaaccat gtctcttctt
     1141 gcgttttctc ccaaatcatt tggtcccaag tcaaagtacc ctgctctctc atgcgagatg
     1201 gtggtaggct gtccgttctc gtccaggtgg aaaaggaaaa actcacactc cggacctaca
     1261 ttaaatatat agcccatctg ctttgccttt tcaatctgtt tctttagtat atagcgcggg
     1321 tctccctcaa acggtgtacc atccgccata tagacatcac agatgattcg tgcgaccttg
     1381 ccctgctgtg gtctccatgg aaaaatcata aaagtgtcaa gatccgggtg cagatacata
     1441 tctgactcct caatcctcac aaagccctct attgacgagc cgtcgaacat acacttgttg
     1501 tcgagtgcct tctcaagctg gctggctgta attgccacat tttttaatgt tccaaagata
     1561 tccgtaaact gtaaacggat aaactcaata tcctcctcat ccactatgcg gataatatca
     1621 ttcttactgt attttgccat tttaaatctc cttattattg caaaaagcta tgaccttatc
     1681 gtactccatc tcaccgccaa acaaatcaag agcactgcct attgtcacat tcagatggtt
     1741 ctgtccaagc tgcctgatgc attttaaatc gtcaaaggat gacacaccac ctgcatatgt
     1801 gaccggtatt cttccccagc tgccaagcat ggcgacaagc tccttttcaa tgccgttcac
     1861 cctgccctca acatccactg catgtaccag aaactcgcag caatatgagg atagcttatc
     1921 caaaagctct gtggtcactg cctcatccgt gaagttttgc cagcggtcag ttactatata
     1981 atactgtcca tcctttttgc gacatgacag atcaagcacc agcctgtcac tgcccacagt
     2041 atctttaagc ctgtttagat ttgcatagtt tatcctgccg tctttaaata cgtaggatgt
     2101 cactataact gctgctgcac cggcatctat aaactccatc gcattgtctg cggtgatacc
     2161 gccacctacc tgtaagccct gcggatacgc ttcaagcgcc ataacagcct gctcctttgt
     2221 atcctcataa tactcgctgt ccacagaatt gagcagaatg atatgaccgc cctttatgcc
     2281 cgatttccta tacatatctg cataaaaccg gctgtcctgc tctgaaacga agttctcact
     2341 ggcaaaatcg cccctgtctt ttaatgaacc accgacaatc tgctttactt ttccattatg
     2401 aatgtcgatg catggtcgaa attccatatt tttctccccg gtttagttta ttgtggctca
     2461 aaaatcgtta ctatccacgc cttagtgtga catgcaaaca ttgccttaaa gccacttaat
     2521 ataaaatata acataatttt tactttattt gcaaaagact tacattagtt ttactaataa
     2581 ataatataat aatttcatat cgggcttata actgcttgac acccactctt taatttgcta
     2641 tcatcataca aattacatta gtatataaag tataccagta taataacata agaaagagag
     2701 gaatttaaaa atgggtacta ttatcggcga aggaattaca tttgatgacg ttctcttagt
     2761 tccacaatat tctgaggtaa caccaaatat gattgagctt cagacacatc ttactaagaa
     2821 gattgtcctt aacattccta tgatgagtgc tgcaatggat acagtcacag aatcaaaaat
     2881 ggctatcgca atggccagac aaggtggtat cggaatcatc cacaaaaata tgtctattga
     2941 ggcacaggca gacgaagtcg ataaggtaaa gcgttcagaa aacggcgtta ttaccgatcc
     3001 gttctatctt tctccagacc atacattaca ggacgcagac aaccttatgg ccaagttcaa
     3061 gatatcaggt gtaccaatca caaaggacgg caagcttgtc ggcatcatca caaaccgtga
     3121 tctgaagttt gagacagact tcacaaagaa gatttcagag tctatgactt ctgagaatct
     3181 cattacagct cctgagggca tcaccctcga tgaagcaaag aagattcttg caaaggcaag
     3241 aaaggaaaag ctccctatcg tagacaagga ttacaacctc aagggactta tcactataaa
     3301 ggatatcgag aagcagatta agtatcctct ttcagcaaag gatgatcagg gccgtctcct
     3361 ctgcggtgcc ggagtaggta tcacaggcaa catgatggag cgtgttgatg ctcttgttaa
     3421 gtctcatgtt gatgtcatcg ttgttgactc agctcacgga cattcaaaga atatacttga
     3481 ggctgttaag aagatcaagg ctgcttatcc ggatcttcag ataatcgccg gaaatatcgc
     3541 aacaggtgct gctgccaagg ctcttatcga ggccggtgca gatgcagtaa aggttggtat
     3601 cggacctggt tctatctgta ccacacgtgt cgttgccggt atcggtgtac cacagattac
     3661 agctatcatg gactgttaca aggttgctaa ggagtatgga gttcctgtta tcgcagatgg
     3721 tggtatcaag tactcaggtg atatgacaaa ggctctcgca gccggtgcca acgtatgtat
     3781 gatgggctca atgttcgcag gctgtgatga ggctccggga accttcgagc tttatcaggg
     3841 aagaaaatac aaggtatatc gtggcatggg ttctatcgct gctatggaaa acggaagcaa
     3901 ggaccgttac ttccaggagg gcgctaagaa gctcgttcct gaaggagttg agggtcgtgt
     3961 agcttacaag ggaactcttg aggatacagt attccagctt gttggtggta tccgctcagg
     4021 aatgggctac tgcggttgca agacaatcga agatcttaag gagaacggac agtttgtcaa
     4081 gattactgct gcttctctca aggagagcca ccctcatgat atccatatca caaaagaggc
     4141 tcctaactac agcacagacg ataaataaac acatttatta attcatatca cgaggtaaaa
     4201 tatttggacg ctgacgacat atcgcagatt atattcttat taatactatt ggcactctcc
     4261 gcattttttt catcttcgga gaccgctctt actaccgtaa acaaaatacg tatgagaacc
     4321 ctggctgagg ctggcaacac aaaagccaaa aaggtgctca aggtgactga gaactctcca
     4381 aagatgctct cagccatcct tatcggcaac aacatcgtaa atctttcagc atcatcgctc
     4441 accacctcgc ttgccatcaa gctgtttggc aacgttggag caggtgtcgc aaccggaata
     4501 cttacattcc tcatactgat cttcggagag gtctctccga agacacttgc gaccataaag
     4561 gctgacaaga tttcactctc aatcgcaggc tttatatcag tgctcatggt agtgctgaca
     4621 cctgtaatat ttatcatcaa caagctttca ctcggagtca ttttcctctt cggaataaga
     4681 caaagcgatg ccaagcgcgt catgaccgag gaggagcttc gtacaatcgt tgatgtagga
     4741 caggaggacg gtgtcatcga ggatgaagag cgtgatatga ttcacaatgt attcgatttt
     4801 ggagatgccg aggccaagga ggtcatggtt ccgcgtattg atatgacgtt tgtccatgtt
     4861 gattctactt atgatgattt gatttccata ttcagagagg ataaattcac cagactccct
     4921 gtgtatgacg aaagcactga caacgtaatc ggtatcgtaa atatgaagga tctgctgctc
     4981 cttaaggatg aggataaaga gcatttttcc atcaggaata tcatgcgtga gccttacttt
     5041 acatatgagc acaagaatac agcgaatctg tttctcgaga tgcgcgaatc atctatatcg
     5101 cttgccattg tcttagatga atacggtgtc acagccggac tcatcacact tgaggatctc
     5161 ttagaggaga tagtcggtga gattcgtgat gaatacgact ctgacgagca ggatgatatc
     5221 acaagaataa atgactacga atacatagtt cttggctctg caaatctcga ggatgt
//
LOCUS       DPUF01000186           16943 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_821, whole
            genome shotgun sequence.
ACCESSION   DPUF01000186 DPUF01000000
VERSION     DPUF01000186.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 16943)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16943)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16943
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_821"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="DHV81_06610"
     CDS             complement(<1..914)
                     /locus_tag="DHV81_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCI94133.1"
                     /translation="MIDDYSGLQKAAILLITLGPEKASAIFQHLKEDEIESLTLEIAN
                     TRSVSPQDKESVLDEYYQVCLAQQYIAEGGIGYAKELLEKALGSEKAQGVISKLTASL
                     QVRPFEFVRKTEPAQLLNFIQDEHPQTIAMILSYLSSSQAAMILGALPPEKQADVARR
                     IAQMDRTSPDVIKEVERVLERKLSSLVNQDYTIVGGIDSIVNILNSVDRATEKRIMES
                     LEVDEPELAEEIRKKMFVFEDILLLDDRSIQRVLRDVENADLELALKSSTEELQNVIF
                     RNLSKRLAAMIKEDMDFMGPVRMKDVEEA"
     gene            complement(921..2522)
                     /locus_tag="DHV81_06615"
     CDS             complement(921..2522)
                     /locus_tag="DHV81_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HCI94134.1"
                     /translation="MPERLKAILDKITAWWKKFNTKQRSVLISIVAVVIVALVILGVV
                     ISRPTQVELVEAQSASEASTIKGVLEDNNISYEVDSKLVFFVNKSDEVNAIMALGDNG
                     IPAQAYSIDNVTDGSFSTTEADKQKKYQVYLEDKLKDHLEKQSYVNEASVDITMPDKD
                     GTILKSKEEATAAVTLGLKDSITDDQAYAIARLVATALGNDTTKGITIIDKKANVLYS
                     GGDSDTSAGLATSQLSYKQKLEEQVKNEVKKTLESSNIFSNIEVGMNLDIDFSKTETA
                     QKNYTLPDGQTDTGLLDSESNYQSDATNGQAAVPGTDSNSDDTTYTTQDGTPSESSIT
                     DNDKKYLNDEKITTSKNDGGSIKYDSSSITVVATRYKVYDESTLEKNGKLKDTSWEEY
                     KAKHADPVKVTDVDQDLVTSVQNATGISQISFLVYEQPEFVDKASGVRSLSDILQIAI
                     TVLIFALLGFVVFKSTRSSEEEPKEEELSVDSLLEQTAENNEPLEDIGYNEKTETRLL
                     IEKFVDENPEAAAQLLRNWLNEDWE"
     gene            complement(2536..2850)
                     /gene="fliE"
                     /locus_tag="DHV81_06620"
     CDS             complement(2536..2850)
                     /gene="fliE"
                     /locus_tag="DHV81_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCI94135.1"
                     /translation="MDISNINGISTGDIKSFFNNTSLTKTDKTDSFSDVLSAAMGSIG
                     ETNDLQNAAEQEEVRFALGESDNTHDLLVAETKAAVALQYTVAVRDKIIDAYKELMQM
                     SI"
     gene            complement(2883..3323)
                     /gene="flgC"
                     /locus_tag="DHV81_06625"
     CDS             complement(2883..3323)
                     /gene="flgC"
                     /locus_tag="DHV81_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCI94136.1"
                     /translation="MGLFQAFNISASGMTAERFRTDIIAQNIANVNTTSTKEGTPYRR
                     KIVTFSEKNVTPFSQIYSSSKKAAVGNGVKVSSVTSDYSTDFIKEYDPSNPDADADGY
                     VSYPNVNTVTEMTNLIDATRAYEANATAFEASKSMAQNGLQIGK"
     gene            complement(3345..3731)
                     /gene="flgB"
                     /locus_tag="DHV81_06630"
     CDS             complement(3345..3731)
                     /gene="flgB"
                     /locus_tag="DHV81_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCI94137.1"
                     /translation="MINSTAFDYVNVLTKAADASYQREAILANNISNVDTPGYKRKDL
                     NFEGVLSQELGRCKHQSLDKKISELDTSKLTANVYTDHSNYSYRMDGNNVDIDTENVE
                     LASEQIKYEALTSSISSQFSRMKSVL"
     gene            complement(3952..4728)
                     /locus_tag="DHV81_06635"
     CDS             complement(3952..4728)
                     /locus_tag="DHV81_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCI94138.1"
                     /translation="MGVQLLDKTRKINKLLHNNNSSKVVFNDICQVLTGVLDSDILVI
                     SKKGKVLGSSICKGVDELHELIVDEVGGYIDTELNERLLGILSTKENVNLETLGFGED
                     TRMYKAIITPIDIAGERLGTLFEYRSDREYDIDDIILTEYGTTVVGLEMMRSVNEENE
                     EEKRKVAIVRSAINTLSYSELEAILHIFDELDGNEGILVASRIADRVGITRSVIVNAL
                     RKFESAGVIESRSSGMKGTYIKVVNDVVFDELDRIRNKNK"
     gene            complement(4735..6830)
                     /locus_tag="DHV81_06640"
                     /pseudo
     CDS             complement(4735..6830)
                     /locus_tag="DHV81_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742655.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
     assembly_gap    5167..5236
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6472..6549
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6830..7909)
                     /gene="dprA"
                     /locus_tag="DHV81_06645"
     CDS             complement(6830..7909)
                     /gene="dprA"
                     /locus_tag="DHV81_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCI94139.1"
                     /translation="MKFKYWLTSLTDISNAKKKLLHDCSITAEKLFFMPKNELFDILF
                     FTDDDRKIILESRASYDVEKEWILFQQKDIGFVTMEDEAYPDKLRNIHNPPYSLFYIG
                     VLPDKSRKSVAIVGARGRSAYGCEVAKVLAAALSRQGIQIISGMARGIDRDAHMGCLE
                     AGGNTYAVLGSGADTCYPKENRFLYDKIKVFGGIISELMPGTDPQKMFFPMRNRIISG
                     LSDIVVIVEAKKRSGSLITADFAMEQGKDVYVIPGRITDTLSYGCNSLIKQGAGIIYN
                     IDEFVSDITMTGFTECTQMDFRKNILDKKEALVYSLLDFCPIAIGTLSQKVPYELSEL
                     FEVLEDLIQKGFVKETIPNYYIRSL"
     gene            complement(7906..9441)
                     /locus_tag="DHV81_06650"
     CDS             complement(7906..9441)
                     /locus_tag="DHV81_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HCI94140.1"
                     /translation="MYSTVRTAVLDGISAIPVQVEVDISTGMPVFDMVGNLTSEVREA
                     KERVKTALHSVGIILPAKRITVNLSPANIKKTGTGFDLPIAVAILTAMGVIDADSIKD
                     CTLAGELNLNGEILPVSGILPIVFDEYNKGIGKFIIPKQNENEGRLVCGAKIFGISHL
                     NDVIAQFDETAFTCQKTGMAHELQMDEKYADFCDVNGQKFIKRACEVAAGGMHNILLV
                     GPPGAGKTMIAERMPSILPPLSENERLELSKIYSICGLLDNDSSLRDNRPFRNPHYSV
                     TQAALIGGGTRINPGEISLAHNGVLFLDELAEFKGGLLDLLRAPLEEHCIRLSRAGRN
                     VTYPANFLLFGAMNPCSCGYYPDMQRCRCSEPTLRRYFDKVSQPIIDRIDICVEASPL
                     SFEDINSTTSNESSADIRKRVMYCHELQKERFKGESFSYNSKISTDKLEKYCFLGSRE
                     KSYMENMFDKLGLTARTYHKILKVARTIADLDGCENIKTKHLNEAICYRSINEKFWGG
                     AVS"
     gene            complement(9532..10224)
                     /locus_tag="DHV81_06655"
     CDS             complement(9532..10224)
                     /locus_tag="DHV81_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HCI94141.1"
                     /translation="MAKIISQNTDLNIIYYKQIMTGCYVNTFLSPLSLERETECLKKV
                     AQNDKEAKDELILHNMRLVAHVTKKYAVSEDEMEELISIGTIGLIKAVSSFKADYGNR
                     FATFAIRCIENEILMHFRSRKKSRGDVSIFEPIGTDKEGNQIHLVDVIENGQSDVVSD
                     IEVSESLKILRQNMRSVLSDREYYIITKRFGLDGEKELTQRQIAKTLSISRSYVSRIE
                     RAGLKKLRKLLE"
     gene            10223..11110
                     /locus_tag="DHV81_06660"
     CDS             10223..11110
                     /locus_tag="DHV81_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCI94142.1"
                     /translation="MNRKIDYKINENDTGKTVEGFLKERGYTHQVLVRLKQTDHGILR
                     NGIWAYTNERLCKDDLITVQIVENECSDNIAPVNLPLDIAYEDDDIIVINKPFDMPAH
                     PSAGNHDNTLANALMYYYGSQNKPFVYRCINRLDRDTTGLTIVAKHALAGGILGNAVA
                     KRAIHRTYYAVCSGCTPACMRISAPIARKDASTIERCVDFKRGENAVTDFIRIKYNPD
                     NNLSLVKLRLLTGRTHQIRVHMKYIGFPLIGDFLYNPDYTYISRQALHSGRLVFTHPV
                     TGQKMNLISDIPSDMKSLF"
     gene            complement(11087..11689)
                     /locus_tag="DHV81_06665"
     CDS             complement(11087..11689)
                     /locus_tag="DHV81_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCI94143.1"
                     /translation="MKAIRHFITITHHKILVMQGCFRIGLYKQGLLHDMSKYSPTEFL
                     VGCRYYKGYMSPNNAERADRGYSSAWLHHKGRNKHHLEYWIDYAVSKPGDDKTHTKME
                     GMKMPIRYVCEMFIDRVSASKNYQKEKYTDKSALEYYEKSVDHYMIHPDTKAMLEYLL
                     VMLSVKGEEYTYSYIKKEVLKGHIPYEKDKINQLEQGLHV"
     gene            complement(11694..>12293)
                     /locus_tag="DHV81_06670"
     CDS             complement(11694..>12293)
                     /locus_tag="DHV81_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen operon protein GlgX"
                     /protein_id="HCI94144.1"
                     /translation="RKRFIREIRKLKWKDAMLLLFLAQGVPMIMAGDEICNSQDGNNN
                     AYCQDNPTGWVNWSNEHSRRENIRFLTRLIKFRDEHPVISCPKPFKFSDYKAVGYPDL
                     SYHCKNAWIGECDATKLCVGVMYCGDYNEVNKDYVYVAYNFYSSREKLALPKLKKGMK
                     WYAVCDSTLKEPFYDTPVLCENQQYADVSPQSVYILIGR"
     assembly_gap    12294..12494
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12495..13546)
                     /locus_tag="DHV81_06675"
     CDS             complement(<12495..13546)
                     /locus_tag="DHV81_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen operon protein GlgX"
                     /protein_id="HCI94145.1"
                     /translation="MNFRLTKGNFLKTGAYIEGDSAVFTFAAEKEDECSIVLLDKSGN
                     TACVIDIPAEYSTGSLYSVRLHGFCGNEYAYYFRINGKRCIDPYATRIFGREKWNDKT
                     RSDNDYEIACGIDSSDFDWKYDSFPEITRDRMKLYKLHVRGFSMDVSGNKKHKGTFEA
                     VSDRINYIKKLGFTSILLMPVYEFEEMTIPVKHDIPEYAKPKYSLKDEQSVHTENDKV
                     NFWGYTSGNYFAVKASYASDASDASNELRRLVERLHKNGMECIVEMYFPDRTDHNLIL
                     DALRYWVMSFHVDGFKLLGDRLPVTAIVQDALLSRTKILYDGFDMSVVPQNRTYENLY
                     IDKEEYQFAARMMLNH"
     gene            13670..13981
                     /locus_tag="DHV81_06680"
     CDS             13670..13981
                     /locus_tag="DHV81_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCI94146.1"
                     /translation="MAEKATPVTPDEADDIRVTLDLDNGSVECKIITIFDADHKDYIA
                     LLPLDKNGNENESGEVYLYRYFEDEEGLPRIENIESDDEYEAAADRFDELLDDEMFDD
                     I"
     gene            complement(13978..15863)
                     /locus_tag="DHV81_06685"
                     /pseudo
     CDS             complement(13978..15863)
                     /locus_tag="DHV81_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742663.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
     assembly_gap    14662..14751
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15885..>16943)
                     /locus_tag="DHV81_06690"
     CDS             complement(15885..>16943)
                     /locus_tag="DHV81_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCI94147.1"
                     /translation="KHCLHLSKEEIEANLAAGKPYVIRQNNPTEGTTTFEDEIYGDIT
                     VDNAELDDMILIKSDGYPTYNFANVVDDHLMGITHVVRGNEYLSSSPKYNRLYEAFGW
                     DVPVYVHCPLITDEEHHKLSKRCGHSSFEDLLDQGFLTEAIVNFVALLGWSPSDNQEI
                     MSLPELVEKFDYHHMSKSPAVFDFTKLKWMNGEYIKKLDFDKFYEMALPYIKEVVTKD
                     YDLKKIARMVQTRIEIFPDIKEHIDFFEELPDYDIAMYTHKKMKTNPETSLEVLNDVV
                     PILESCDDFSNDALFETLKAYATEKGYKIGYVMWPIRTAVSGKQMTPGGATELMEVFG
                     KEESIARIKKGIEKLSNN"
BASE COUNT         4429 a         3875 c         2981 g         5219 t
ORIGIN      
        1 gcctcctcaa catccttcat acgtacaggt cccatgaaat ccatatcctc ttttatcata
       61 gcggcaagtc gttttgaaag attcctaaat atgacatttt gtagttcctc tgtagaactc
      121 ttaagtgcaa gctcaagatc cgcattctcc acatcgcgga gcactctctg gatagacctg
      181 tcatccagga gaaggatatc ctcgaataca aacatcttct ttctgatttc ttctgcaagc
      241 tcaggttcat caacctcaag tgactccata atacgctttt ctgtagcacg gtctacactg
      301 tttaatatat tgacaataga atctatgcca ccgacaattg tatagtcctg attgacaaga
      361 gatgaaagct ttctctccag cactctctcg acctccttta tgacatcagg tgatgttctg
      421 tccatctggg cgatacgtct ggcaacatca gcctgctttt ccggaggcaa agcaccgagt
      481 atcatggcag cctgtgaaga cgacagataa gaaagaatca tggctattgt ctgaggatgc
      541 tcgtcctgta taaaattaag cagctgtgca ggctcagtct ttctgacaaa ctcaaatggt
      601 ctgacctgta atgatgctgt aagcttggaa ataactccct gagccttttc actgccaaga
      661 gctttttcaa ggagctcctt ggcatagccg atgccgccct cagcaatata ctgctgcgcg
      721 agacacacct gataatattc atccaaaaca gattctttat cctgcggaga gacgcttctg
      781 gtgtttgcta tttcgagtgt cagactctct atctcatctt ccttcaaatg ctggaagatg
      841 gctgaagcct tttcaggtcc aagtgtaatt aaaagaatag cagctttttg taaaccactg
      901 taatcatcta tcatatgtaa ctactcccaa tcctcattaa gccagttacg caacagctgc
      961 gcagccgctt cgggattttc atcaacaaat ttttctatca gaagacgtgt ctccgtcttc
     1021 tcgttatatc caatatcctc aagaggctca ttattctcag cagtctgttc taaaagagaa
     1081 tctacagaca gctcctcctc ttttggctct tcctcactgc ttcttgtgct cttaaatacc
     1141 acaaagccaa gcagtgcaaa aataaggaca gtgattgcaa tctgtaaaat atcactcaac
     1201 gaacgcacgc cagatgcctt gtcaacaaac tccggctgct catatacaag gaatgaaatc
     1261 tgtgagatac ctgttgcatt ctgcacggaa gttacaagat cctggtctac atccgtcacc
     1321 ttgaccggat ctgcatgctt tgctttgtac tcctcccagc ttgtatcctt gagctttccg
     1381 ttcttttcaa gagtgctttc atcatatacc ttatacctgg tggcaactac ggtaattgaa
     1441 gatgaatcat acttgattga gcctccatcg tttttacttg tagtgatttt ttcatcattt
     1501 aaatactttt tgtcgttatc tgtaattgac gactctgatg gagtgccatc ctgtgtagtg
     1561 tatgtagtat catcgctgtt tgaatcagtt ccgggaaccg cagcctgacc attggttgca
     1621 tccgactggt aattcgactc actgtccaaa agacctgtat cggtctgtcc atcaggcaga
     1681 gtgtaatttt tctgggctgt ttcagtctta ctgaaatcta tgtccagatt catgccaact
     1741 tcaatattcg agaaaatatt tgagctctct aaggtctttt tgacttcatt tttcacctgc
     1801 tcctccagct tttgcttata agaaagctgt gatgtcgcaa gacctgccga ggtatcggaa
     1861 tctccgcccg agtaaagtac attggccttt ttgtcgatga tagtaatgcc ctttgtggta
     1921 tcgtttccaa gagctgttgc aacaagacgt gctattgcgt aggcctgatc atctgtaatc
     1981 gaatccttaa gtccaagagt gacagccgca gttgcctcct cttttgattt aagaatggta
     2041 ccatccttgt caggcatagt gatatcaacg cttgcttcat ttacgtatga ctgtttttcc
     2101 agatgatcct taagcttatc ctccagatac acctgatact ttttctgctt atcagcctcg
     2161 gttgttgaga agctgccgtc tgtgacattg tctatcgagt acgcctgcgc agggatgccg
     2221 ttatcaccca gtgccataat ggcatttacc tcatctgatt tattgacaaa aaacacaagc
     2281 ttgctgtcca cctcatagct tatattgtta tcttcaagta ctccctttat tgtggaagcc
     2341 tctgatgcac tctgtgcctc gaccagctca acctgtgtag gtcttgatat gactacgcca
     2401 agtattacaa gagccactat aaccacagct actatgctta tgagcacgga gcgttgctta
     2461 gtattgaatt ttttccacca tgcagttatt ttatccaaaa tagcctttaa acgttctggc
     2521 attttctatt actccttata ttgacatctg cataagttct ttgtatgcgt caattatttt
     2581 atccctgaca gcaacagtgt actgcaatgc cacagccgcc tttgtttccg caacaagcag
     2641 atcatgtgta ttatcagact cgccaagtgc aaatctcact tcctcctgct cagctgcatt
     2701 ctgcagatca tttgtctcac cgatgcttcc catggcagca gaaagtacat cagaaaatga
     2761 atcagtcttg tcagttttcg taagagaggt attattaaaa aaagatttaa tatctcctgt
     2821 tgaaattcca ttaatgtttg aaatatccat caaaaaagct cctttatgta tgtatcagcc
     2881 ggctatttgc ctatttgtaa gccattttgt gccattgact tgcttgcctc aaaggcagtt
     2941 gcattggctt catatgcacg tgtggcatct atcagattgg tcatctctgt gactgtattg
     3001 acatttggat aagagacata accatctgca tcagcatcag gatttgatgg gtcatactcc
     3061 ttgataaaat cagtagaata atctgatgtc acgctgctta cctttacacc gtttccaaca
     3121 gcggccttct ttgatgaaga gtatatctgt gaaaaaggtg tgacattttt ctcggaaaaa
     3181 gtcacaatct ttctacggta cggtgtgcct tcctttgtgg acgtagtgtt tacattggca
     3241 atattctgtg caatgatatc tgtcctgaag cgttcagccg tcatgccgga cgcacttata
     3301 ttaaaagctt gaaataatcc catatcatta aacctccata attactacag cacgctcttc
     3361 attcgtgaaa actgcgagct tatgctgctt gtaagtgctt catacttaat ctgctcagac
     3421 gcaagctcga cattttcagt gtctatatcc acgttattgc catccatcct gtatgaataa
     3481 ttggaatggt ctgtatatac attggccgtc agctttgagg tatcaagctc acttattttt
     3541 ttatcaagcg actgatgctt gcatctgcca agctcctgtg acagtacccc ctcaaaattt
     3601 aaatccttgc gcttgtagcc cggggtatcc acattcgata tattgtttgc aagtatagct
     3661 tctctttggt agcttgcatc agccgctttg gtcaatacat tgacataatc aaaggctgtt
     3721 gagtttatca taaatattcc tccaatacac tttttgcata ttataaaaaa tactatataa
     3781 gtactctaat atatatatta taaagtaaaa tcgacaaaac acaagatgtt tttgttgttt
     3841 tttacattat attgtattat tttgtcgaca gacgcactat atatatgccg aaaacataga
     3901 aaagacctat gttctttcaa acataagtcc ttaaaatata cttaaattta tttacttgtt
     3961 tttatttcgg attctgtcaa gctcatcaaa gactacatcg tttactactt tgatatatgt
     4021 gcccttcatg ccggatgagc gtgattcgat aacgcctgca ctctcaaact tgcgaagggc
     4081 atttacgatt accgagcggg taatgcctac tctgtcggct attctgctgg caacaagtat
     4141 accctcattg ccgtcaagct catcaaagat gtgaagaata gcctcaagct cagaataaga
     4201 aagtgtattg atagccgaac ggacaatcgc tacctttctc ttttcctctt cattctcctc
     4261 attcacggat ctcatcatct caagcccaac cactgtcgtg ccatactctg taagaatgat
     4321 atcgtcaatg tcatactctc tgtcacttct gtactcaaaa agagttccaa gcctctcgcc
     4381 ggctatatca atcggtgtga taatagcctt gtacattctt gtatcttcac caaagccaag
     4441 tgtctcgagg tttacatttt cctttgttga aagtatgcca agaagtcttt cattgagctc
     4501 agtgtcaata tagcctccaa cctcatctac tatcagttca tgaagctcat ccacgccttt
     4561 gcagatactt gagccaagca ccttgccctt tttgctgatt acaagtatgt ctgagtcgag
     4621 cacaccggtg agcacctgac atatatcatt gaacacaacc tttgatgaat tgttgttgtg
     4681 aagaagctta ttaatcttac gtgtcttatc taataattgt actcccatta attgttattc
     4741 ctctctttcg ggctttttat ctgttacgta gccgcactgc tcatctgcgc aggcaatctt
     4801 atttcccttt ataatcatat atcctccaca acgagggcat ttttctgcaa ctggtctgcc
     4861 ccagctcata aaatcacact cagggttgtc aatacagcca tagtattttc ttcccttcct
     4921 tgttttctta agtacaacat ccttaccgca tttagggcaa ttgacgccga ttttttcaag
     4981 ataaggcata gtgaatctgc agtcaggaaa tcccggacat gccaaaaact tgccgtgagg
     5041 tccgtatttt ataaccagat tgcgtccgca ctcagggcag atttcatctg tcacctcatc
     5101 atgtattgta accttctcaa gctcattttc agccttgtcg attgcatctt taagatcagg
     5161 ataaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnngact ccatattggc tgtaaaatgc tcatcgacaa tagtaggaaa
     5281 tgattccttc ataatagaat taacaacctc tcctatctct gtcacataga gatttttact
     5341 ctccttggta atatagcgtc tggcaagtat tgtagatatt gttggagagt aggtactcgg
     5401 acgtcctatt ccaagctctt caagcgtctt taccaaagaa gcctcagtgt aatgtgccgg
     5461 gggctgggta aaatgctgct taggcaaaag ctcctcaact gtaagaaacg attcttctgt
     5521 cagaggattt ttaagaacag gctgcttttc tttctttgca tcgtctgcgg caatatatgc
     5581 aagctcaaaa ccatcaaata acagtcttga ggtagcaaag ctgaatacat aatctccgac
     5641 tgaaagcttt actgtagtag tctcgtactc ggattttgcc atacggcttg cagtaaaacg
     5701 cttccagata agctgataaa gcctgaactg gtctctcgac agtgactcct taatctcaga
     5761 aggcactctg tttatatcag ttggcctgat tgcctcatgg gcatcctgga tatttttact
     5821 gctcttcttt gttccctcac cggtggaaag atatttgtct ccatagcttt ttgaaataaa
     5881 atcctttgcc atggcctcag cctcatctga tacacgcaca gaatcggtac gcaggtatgt
     5941 gatgatacct accgtaccct gtccctttat gtctactccc tcatagagct gctgagctat
     6001 acgcattgtc tttgaaatcg gaaaattcaa agcctttgaa gcctcctgct gcaaagtact
     6061 ggtcgtaaaa ggaagcggag ctttattgct gcgctgtcct tttttaatat tttgcacttt
     6121 gcaatccttt ccctttattt cgttaagcac cttatccatc tcattctttg aggaaatggc
     6181 tatcttattg tcgcccctgc cgtatagctt tgcagtgatc ggcttcttct caccctccac
     6241 atcaaccgtg gcatcaaggc tccagtactc ctcagggata aatgcattga tttcatcctc
     6301 cctgtcacag ataatgcgaa gtgcaacaga ctgcactctt cccgcactaa gccctctctt
     6361 aaccttggcc caaagcagcg gactgatttt gtaacctact atacgatcca aaactcgtct
     6421 ggcctgctgt gcgtccacaa gatccatatc tatttttctt ggatgcttta annnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnna gatgccatga gatggcttct ccctcacggt cgggatcggt tgcgagatat
     6601 atcttatcgg ccttcttagc ctccttgcga agcttagcga gtatttcacc ctttccgcgg
     6661 attgtgatat attttggttc aaaatctccg tccacatcta ttccaaggct gctctttggc
     6721 agatccctca catgaccgtt tgatgccatt acctcatagt tttttcccaa gaatttcttt
     6781 atagtcttta cttttgcagg cgactctacg ataaccagat atttagccat tataaactcc
     6841 ttatatagta atttggaatt gtctccttaa caaatccttt ttgtataaga tcttctaaca
     6901 cttcaaatag ttccgataat tcatacggca ctttttgaga caatgtcccg attgcgatag
     6961 gacaaaaatc gagtaaacta tacaccaatg cttctttttt atcaagtata ttttttctga
     7021 aatccatctg tgtgcactct gtaaacccag tcattgtaat gtctgagaca aattcatcaa
     7081 tattataaat aatgcctgcg ccctgcttta tcaggctgtt gcagccgtag cttaaggtgt
     7141 cggtgatacg ccccggaatg acatatacgt cctttccctg ctccattgca aaatcagccg
     7201 taatcagtga accgcttctc ttttttgcct caactataac tactatatcg gataacccgg
     7261 aaattattct gtttctcatc ggaaaaaaca tcttttgcgg gtcggtgccc ggcataagct
     7321 cgctgattat gcctccaaag acctttattt tatcatatag aaagcggttc tctttcggat
     7381 aacaggtgtc tgctccggag ccaagaactg catatgtatt tccaccagcc tccagacacc
     7441 ccatatgggc atctctgtct attcctcttg ccatgccgct gattatctgt atcccctgcc
     7501 ttgaaagtgc tgctgccagt acctttgcca cctcgcagcc atatgcgctt ctgccccgtg
     7561 ctccgactat agcgacactt tttcttgatt tgtcgggaag aacgcctatg taaaaaagac
     7621 tatacggcgg attatgtata tttctaagct tgtccggata agcctcatcc tccattgtta
     7681 caaagccgat atccttctgc tgaaataata tccactcctt ctctacgtca taggaagctc
     7741 ttgattcaag gattattttt ctgtcatcat ctgtgaaaaa caaaatatca aaaagctcat
     7801 tctttggcat aaaaaacagc ttttctgccg ttatactgca atcatgcaga agctttttct
     7861 tcgcattgct tatatctgta aggcttgtaa gccagtattt gaacttcatg atacagcacc
     7921 tccccagaac ttctcattga tgcttctgta gcatatagct tcgttgagat gctttgtctt
     7981 tatgttttca cagccgtcca gatcagctat tgtacgcgca accttaagta tcttgtggta
     8041 tgtgcgtgct gtcagtccaa gcttgtcaaa catattttcc atgtagcttt tctctcgact
     8101 gccgaggaaa cagtattttt ccagcttgtc agttgatatt tttgaattat aagaaaagct
     8161 ctcaccttta aacctttctt tttgaagctc gtggcagtac ataacacgct ttcttatgtc
     8221 cgcagatgat tcattggatg tagtactgtt tatatcctca aagctaagcg gtgacgcctc
     8281 cacacatatg tcaattctgt ctattatagg ctgtgagacc ttatcaaaat atctgcgcag
     8341 agtcggctct gagcagcggc acctttgcat atcaggataa tagccgcagc tgcatggatt
     8401 catcgcacca aaaagcagaa aattagccgg ataagtcacg tttctgccgg ctcttgaaag
     8461 cctgatgcag tgctcctcaa gcggagccct gagcaggtcc aacaagccgc ccttaaactc
     8521 agcgagctca tccaaaaaca aaacgccatt gtgtgcaagc gatatctcgc ccggattaat
     8581 tctcgttcct ccgccgatta atgcggcctg ggtgacggaa tagtgaggat ttctgaatgg
     8641 tctgttgtct cttaaggagc tgtcattgtc caaaagaccg cagatgctgt atatttttga
     8701 cagctcaagc ctctcatttt cactaagagg aggcagaatg gacggcatac gctctgctat
     8761 cattgtcttg cctgcaccgg gaggtccgac aagcagtata ttgtgcatac cacccgcagc
     8821 cacctcacat gctcttttaa taaatttctg tccgttaaca tcacaaaaat cggcgtattt
     8881 ttcatccatt tgcagctcat gtgccatgcc ggttttctga cacgtaaatg ctgtctcatc
     8941 aaactgtgca attacatcat ttaaatggct tatcccaaat atttttgcac cgcagacgag
     9001 tctgccttca ttttcattct gctttggaat aataaacttt ccaatgcctt tgttgtactc
     9061 atcaaataca atcggcagaa taccgctgac cggcaaaatt tcaccgttta aatttagctc
     9121 gcctgcaagt gtgcagtcct ttatgctatc agcgtcaatc acccccatag ctgttaaaat
     9181 agcaacagct ataggcagat caaagcctgt tccggtcttt ttaatatttg caggcgatag
     9241 atttactgtg atacgcttgg caggcaaaat aatgccaacg ctgtgcagcg ctgttttaac
     9301 acgctccttt gcttctctca cctctgaggt aagatttcca accatatcaa aaacaggcat
     9361 accggttgaa atatcaacct ccacctgtac cggaatcgca ctgattccat ccaaaacagc
     9421 cgttctgact gtactgtaca tatatactcc tattccaatt ggggtaaatg catagacaaa
     9481 tgcacaaaag acatggctct atgataacat atttttgcct tttgtaaaca cctattcgag
     9541 cagttttcgt aattttttca aaccggccct ttcaattctt gaaacatatg acctcgaaat
     9601 gctgagcgtt ttagcaatct gtctctgtgt cagctctttt tcaccgtcaa ggccaaagcg
     9661 tttcgtaata atataatatt ctctgtccga taaaacgctt ctcatatttt gccttaaaat
     9721 cttaagtgac tcagatacct ctatatccga taccacatca gattgtccat tctcaatcac
     9781 atccaccaga tgaatctggt ttccctcctt gtcagtgcct ataggttcaa atatggacac
     9841 atcaccccgg ctctttttac ggcttctaaa gtgcatcaga atctcatttt cgatgcatct
     9901 gattgcaaag gttgcaaaac gatttccata atcagccttg aatgatgaca ctgcttttat
     9961 cagtcctata gtgcctatgg atatgagctc ctccatctca tcctcgctta ctgcatattt
    10021 ttttgtaaca tgtgccacaa ggcgcatatt gtgtaatata agctcatcct tagcttcttt
    10081 atcattttga gccacttttt tcagacattc cgtctcacgt tctaaactaa gaggtgataa
    10141 aaatgtattc acatagcacc ctgtcattat ttgcttataa tatattatgt tcaaatctgt
    10201 attttgtgat ataatctttg ccatgaatag aaaaatagac tacaaaatca atgaaaatga
    10261 tacaggaaaa acagtagaag gctttttgaa ggagcgtggc tacacacatc aggtgcttgt
    10321 caggctgaag cagacagacc atggcatact aagaaacggt atatgggcct atacaaatga
    10381 aaggctgtgt aaggatgatt taatcacagt ccaaattgta gaaaacgagt gctcggataa
    10441 tatagcacct gtaaatctgc ctcttgatat agcatacgag gacgatgata tcatcgtaat
    10501 aaacaagccg tttgatatgc ctgcccatcc ctccgccgga aaccatgaca acacgcttgc
    10561 aaatgcgctc atgtactatt atggcagtca gaataagccg tttgtataca gatgcataaa
    10621 caggcttgac agagatacca cagggcttac aattgtcgca aagcatgcac ttgcaggcgg
    10681 gatacttggc aacgctgtcg caaagcgtgc catccaccgg acatactacg ccgtctgcag
    10741 tgggtgtaca cctgcatgca tgcgcatcag tgctccgatt gcaagaaagg atgcttccac
    10801 catagagcgg tgtgttgatt tcaaacgcgg tgaaaatgca gtaaccgact ttatcagaat
    10861 caaatataac ccggataata atttatcgct tgtcaagcta agacttctta ccggacgcac
    10921 tcaccagata cgcgtccata tgaagtatat cggttttcca ctgataggtg actttttata
    10981 taatccggac tatacatata tatccagaca agcactgcac tcagggcgtc ttgttttcac
    11041 gcaccctgtc acaggacaaa aaatgaacct catatctgat attccatcag acatgaagtc
    11101 cctgttctag ttgatttatt ttatcctttt cgtatgggat atgccctttt aaaacctctt
    11161 tttttatgta gctgtaagta tattcttctc cctttacaga gagcatgaca agcagatact
    11221 caagcattgc ttttgtatcg ggatgaatca tgtagtgatc cacgcttttt tcatagtatt
    11281 ccagcgcact tttgtcggtg tacttttctt tctgatagtt ttttgaggcg cttacacggt
    11341 ctataaacat ctcgcagaca taccttatgg gcattttcat gccctccatt tttgtatgtg
    11401 ttttatcgtc ccccggcttt gaaacagcat agtcaatcca gtactccagg tgatgcttat
    11461 tgcgtccctt gtgatgaagc cacgcagatg aatagcccct gtcggctctc tccgcattat
    11521 tcgggctcat ataccccttg taatacctgc agccgacaag aaactcagtc ggtgaatatt
    11581 ttgacatgtc atgaagaagt ccctgtttat aaagtcctat cctaaagcac ccctgcatga
    11641 ctaaaatctt gtgatgtgta attgttataa aatgtcttat tgctttcata tgtctatctg
    11701 cctatgagga tgtataccga ctgcggtgag acatctgcat actgctggtt ttcacacagc
    11761 accggagtgt cataaaacgg ttctttgagc gtagaatcgc agactgcata ccatttcatt
    11821 cctttcttta gcttaggaag agctaacttc tccctggatg aataaaaatt gtaagctacg
    11881 tatacatagt ctttattcac ttcattgtaa tccccacagt acatcacacc tacacatagc
    11941 tttgtggcat cacactcgcc tatccaggca ttcttgcagt gataggacaa atcaggatac
    12001 ccaacagcct tgtaatcgct gaatttaaac ggctttggac aggaaataac aggatgctca
    12061 tccctgaatt tgataagtct ggttaagaac ctgatgtttt cccttctgct atgctcattt
    12121 gaccagttca cccagcctgt agggttgtcc tgacagtaag cattgttgtt gccatcctgt
    12181 gaattacaga tctcatcacc tgccataatc ataggcactc cctgtgccaa aaacaaaaga
    12241 agcatggcat ccttccactt aagttttctt atctcacgga taaacctctt tcgnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnatatgg tttaacatca tacgcgccgc aaactggtac tcctccttgt
    12541 caatatagag attttcatat gtcctgtttt gcggtacaac cgacatatca aagccatcat
    12601 agagtatctt tgtccggcta aggagagcat cctgcacaat cgctgtaaca ggcagcctgt
    12661 ccccaagcag cttaaaacca tctacatgaa agctcatgac ccagtatctg agcgcatcga
    12721 gtatcagatt gtggtcagtt ctgtccggaa aatacatctc tacgatacat tccatgccat
    12781 ttttgtgcag cctttcaacg agacgcctaa gctcattgga ggcatctgaa gcgtcactgg
    12841 catatgatgc ttttacggca aaataattgc cggaggtata gccccaaaaa ttgaccttgt
    12901 cattttctgt atgcacactt tgctcatcct ttaaggaata cttcggcttt gcatattccg
    12961 gaatatcgtg ctttacaggg attgtcattt cttcaaactc gtatacaggc atgagaagaa
    13021 ttgaagtaaa gcctaatttc ttgatgtaat ttattctgtc cgatacagcc tcaaaggtgc
    13081 ccttatgctt tttgttccct gaaacatcca tggaaaaacc tctaacatgc agcttataca
    13141 gcttcatccg gtccctggtt atctcaggaa agctgtcata tttccagtca aaatcactac
    13201 tgtctatccc gcatgctatt tcgtagtcat tatcacttct tgttttatca ttccattttt
    13261 ctctgccgaa aattcttgtg gcataaggat ctatgcacct ttttccatta attctaaaat
    13321 aatatgcata ctcattcccg caaaaaccat gaagtctcac agagtaaagt gagcctgtcg
    13381 aatactcggc aggtatatca atcacacagg cagtatttcc cgatttatca agtaaaacaa
    13441 tgctgcattc atcctctttt tctgctgcaa aggtgaatac tgcactatcc ccctcaatat
    13501 aagcacctgt ttttaaaaag tttccctttg ttagtcgaaa attcatgcaa caccatccaa
    13561 tcaataattc tagtatttat ttattatact agttttgact ttttttgtat agaataattt
    13621 gatataatat aatagttact ttagattact ataaagaaag aggtacaata tggcagaaaa
    13681 agcaacacct gttacacctg atgaggcaga cgatatccgc gtaacacttg atctggacaa
    13741 cggaagtgtc gaatgcaaga ttatcaccat ttttgatgca gatcacaagg attacatagc
    13801 tcttctaccg ttagacaaaa atggcaacga gaatgagagt ggcgaagtgt atctgtatcg
    13861 ctatttcgag gacgaggaag gtcttccgag aatagaaaac atcgaatccg acgatgagta
    13921 cgaggctgct gccgacagat tcgatgagct tttggatgac gaaatgtttg atgatattta
    13981 atcatctcta ctattatttt gtctaggctc tttactttca cggacaaatc tggaggcttg
    14041 ggctgattta tcatttaccg acttgaccga ataaacagac aagcttgtct ttgcacgggt
    14101 catgccgaca taaaacagcc ttcgctcctc ctctatgtcc ttttcaagca cagccttttt
    14161 atacggcatt atcccttcat ttgcatcaat tatatacacg ttttcaaatt caagcccctt
    14221 tgagctgtga agggttgcaa gtgtcacggc attagggtca ctttctttcg ataaagccat
    14281 aagcttcatc tgctttgtat attcccttat atgctcatac cactcttcat atgtagcaaa
    14341 gcccttggct cctgactgga tttcatccag tatatcaaac aaatcctctt tatttatatt
    14401 cctatactcg gcatattcag ctaaaaagtc gtcatagcca atccctcttc tgatatagtt
    14461 tatgctggca tatggattca tccggcttat aagcttcatg tcatactcaa gcttttctat
    14521 ccgctcagct atccacggct tctcatcaaa cagcttaatc cactcatcaa atgccacagt
    14581 tgcatcacac aggctgtctc ttgacagata ccgcttaggt ctgttcataa tctgcaaaaa
    14641 atcagctctg tctctgctgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtatatta
    14761 tccttggttt tgaatgggat attatagctc ataagctgct ctataagctg tcttggctgg
    14821 gtatttgtcc tgaaaagcac cgcaaagtcg ctgaatacag ctcccgcctt tatcttttta
    14881 tctatatcgc gtataagaaa gatattttcg tctcttctgt tttcaaaatc agcaaacgta
    14941 acaggcgcct ttgacttgct tgccgcaatg attttcttat caaatcgctc cctgttgtgc
    15001 gagatgagat tcaacgaggc ctccactata tatctgccac atctgtaatt agtatctaaa
    15061 aggattcttt ttgcattcgg atagtccttc tcaaacccaa gcattatctc aggctttgac
    15121 cctctgaagc ggtatattga ctggtcatca tctcctacaa taaacagatt attctgtgga
    15181 gcggcaagca tttttataat ctcatactgc agacggttaa tatcctgaaa ctcgtcaatc
    15241 agtatgtatt tatacttgtt ctgccacaat gccagtatat caggtctttc cttaaacagc
    15301 tcataagtat acgagagcat atcatcaaaa tcaataagcc tgttttcctt aagccttgtc
    15361 tcatattttt tatatatatc cctgaatatt tcctcaccac acactccaga gtagaaattt
    15421 tctatatcaa tgcggctgtt tttaatcaga ctgatttcag caagtaaatt gcctataaac
    15481 tcattctcat ctttgtattc aaggcggtag taggataaaa tttccctgat aaagccgtat
    15541 ctcacagact catcagcaat attggcactt gtaaagcgat atgcatattt gagtattgta
    15601 aaaaagacag catgaaaagt accaaaagat acgttcaccc tctcgtcttt catcagtgaa
    15661 ttaaaacgct ccttcatctc atttgcagca gccttggtaa aggtgataac taaaatctca
    15721 gaaggcttca catgatgctt tgttattaaa tttttagtcc tgtgtgtgat aactgcggtc
    15781 ttaccggaac ccggtcctgc taaaacaagc atagcgccat ccttgtgttc gatggcgcca
    15841 agctgggctt tgttaaaatt cataaaatac cctttgttaa tagattagtt attagaaagc
    15901 ttttctatgc ctttcttaat tctggctata gactcctctt tgccaaacac ctccataagc
    15961 tctgtagctc cgcccggtgt catctgcttg cctgaaacag cggtacggat tggccacata
    16021 acatagccta tcttatagcc cttttctgta gcatatgcct taagagtctc aaaaagtgca
    16081 tcatttgaga aatcatcaca gctttcaaga ataggaacta catcgtttaa tacctcaagt
    16141 gatgtctctg ggtttgtctt catcttcttg tgagtataca ttgcaatatc ataatccgga
    16201 agctcttcaa agaaatcaat atgttccttg atatcaggga atatctcaat acgtgtctga
    16261 accattcttg ctatcttctt aaggtcataa tcctttgtga caacctcctt aatgtatggc
    16321 aatgccatct catagaattt gtcaaaatca agcttcttta tatactctcc gttcatccat
    16381 ttaagctttg tgaagtcgaa tacagcaggt gatttgctca tatgatgata atcaaacttc
    16441 tcaacaagct caggaagtga cataatctcc tgattgtctg aagggctcca gccaagaagc
    16501 gctacaaagt ttacaatagc ctctgtaagg aatccctgat ccagtaaatc ttcaaaggat
    16561 gagtgaccgc atctctttga aagcttatga tgctcctcat ctgtgataag cggacagtgt
    16621 acatatacag gtacatccca tccaaatgcc tcataaagtc tgttatactt aggggatgat
    16681 gaaagatact cgtttcctct gactacatgt gtgatgccca tcaggtgatc atctactacg
    16741 tttgcaaaat tgtatgtagg atatccgtca gatttgataa gaatcatatc atcaagctct
    16801 gcattatcta ctgtgatatc tccgtaaatc tcatcctcga atgtggttgt gccctctgta
    16861 gggttgttct gtctgataac atatggcttg ccggccgcga gatttgcctc aatctcctcc
    16921 tttgacaggt gcaagcagtg ctt
//
LOCUS       DPUF01000187            8635 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1485, whole
            genome shotgun sequence.
ACCESSION   DPUF01000187 DPUF01000000
VERSION     DPUF01000187.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1485"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="DHV81_06695"
     CDS             <1..1125
                     /locus_tag="DHV81_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCI94148.1"
                     /translation="CKGGRRIPLCFSIKANPFLLHRLPAGLDHVEVCSPGELEICIAL
                     GVKPERIIYSGVMKEKCDIERAVSYGAGILTCESIRHAALISEVMLECIQEGAYEAEF
                     AETKAHVILRLTSGNQFGMSLEDIEYIISHPDEFKGIAVMGIHYYSGTQKSLRKINKD
                     LEKIKSALTGLKEKYGFEPQLVEYGPGLCVEYFEDDWQEKEKQSLDEAAEVLREFAVE
                     YPLGIEMGRFLAASCGKYYTQVKDLKSTGDANYAILDGGIHHLNYFGQRMAMQVPPIS
                     IYRAAGVELTQLPDIDYTLCGSLCTVADVLVREVRLKKLKLGDVLEFGHCGAYSVTEA
                     PALFLSRQLPAIYAYSKEYGYECLREHIPAAEINLAGKKL"
     gene            complement(1202..2317)
                     /gene="glf"
                     /locus_tag="DHV81_06700"
     CDS             complement(1202..2317)
                     /gene="glf"
                     /locus_tag="DHV81_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HCI94149.1"
                     /translation="MKYDYLIVGSGLYGSIFAHEAKSKGKSVLVVDKRPNIAGNVYTE
                     KQEGINVHMYGAHIFHTNDKRVWNYITQFAEFNRFTNSPVANYKGELYSMPFNMYTFN
                     KMWGVVTPEEAAAKIEEQKKEITGEPKNLEEQAISLVGRDIYEKLVKGYTEKQWGRDC
                     KELPAFIIKRLPVRLTFDNNYFNALYQGIPIGGYTKMVENLLDGIEVRLNTDYLEHKA
                     ELDALADKVVYTGPIDAYFGFKLGTLEYRSVRFENETLDIPNFQGNAAVNYTDRETPW
                     TRIIEHKWFEFGKDEDGNDLPKTIISREYSSEWKAGDEPYYPVNDEKNGQLYAKYKEL
                     ADKETCVIFGGRLGEYKYYDMDTTIASVLDMCEKELG"
     gene            2471..3838
                     /locus_tag="DHV81_06705"
     CDS             2471..3838
                     /locus_tag="DHV81_06705"
                     /inference="COORDINATES: protein motif:HMM:PF13304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94150.1"
                     /translation="MKNMYLVKYSVKGIKSLDKLVSLAFYKKTITKDMDTQGFNIKGI
                     YGMNGSGKSGIMSSIQILKNILTDSNYLNNPISQKTLDSLVNKKLSELFIEVEYVVRT
                     KEKLWLFRYNVALAKKESETYIIKSEELSFKNATTRKENMSVLYKVNDGALDLKYFDT
                     SDKTNSMLAEKTINLLDKSSFCSIAVKKIIDKKIFDKKVQMVVPALVILYNFGTKLHV
                     YLDNEDTHIPFIIAHMMKYGDITYEGNEMVDSLLYEASNMDAESMNMLAAEKNFVNRD
                     SYDYFENEVHQLYEFLHIFKSDLVGIEIEKKEDGDMYVCELVMVYNEYRVNVEFESTG
                     IKKLVKLYMYIREMKRGGIVFIDEFDANLHDVYLCALLEYLMEYGEGQLCFTTHNIGP
                     MDILKRNSNSIDFISGDHRIYSWKKSGNYSPANLYRKGMIEGSPFNVDSIDFIDAFGI
                     QEGDE"
     gene            3838..4359
                     /locus_tag="DHV81_06710"
     CDS             3838..4359
                     /locus_tag="DHV81_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94151.1"
                     /translation="MGMQLIFVLETNKKCKSDWIYIKDTIEYFYNIDANNIKFTQVYM
                     DGKGNFKNKQKEINKNIDQYAKGASNGNSKVICCLDSDDYDINMEAIKFLNEAFKFCN
                     DNKYELIWFCKDIERVYLRKKVVKDEKKKEAGKFKANKTIRNIDAKNLVASKYKDGTS
                     NILNILDKYLERK"
     gene            4373..5554
                     /locus_tag="DHV81_06715"
     CDS             4373..5554
                     /locus_tag="DHV81_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCI94152.1"
                     /translation="MKLPGYYSSGEFAKKAHITKKTVRYYDEHNILKPSYVNENGARF
                     YNDDDFARLQQILFLKYLGFSLDDIKEMTLRNTDSNVMSESLHMQLGLVEERIEQMKL
                     MKSALQEASEVIDEGRSVDWSHMLELVNINEMEQKLKQQYRDASNISARINLHRDFSM
                     NPVSWFSWVFDECSFFEGEKVLEVGCGDASLWTQNIDRIPADMQITLTDISYGMVRDA
                     TRNVGADDKRFTYEVMDAHRLYKPDASYDCVIADHVLFYCDNLDAVCSEILRVLKPGG
                     VFVCSTYSSRHMKEINDLVQQFDDRIELSAERLYEQFGKENGEAILGKYFGEVSWHGY
                     EDELVVEKAEPVIEYVLSCHGNQNRYIVDRYKEFAAFVRKKTEGGFHITKDAGVFIAR
                     K"
     gene            5735..>6300
                     /locus_tag="DHV81_06720"
     CDS             5735..>6300
                     /locus_tag="DHV81_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HCI94153.1"
                     /translation="MKGIILAGGSGTRLYPLTMVTSKQLLPIYDKPMIYYPMSVLMNA
                     GIRDILIISTPQDTPRFKELLGDGHQFGVNLTYEVQPSPDGLAQAFVIGEKFIGDDTV
                     AMVLGDNIFAGHGLNKRLKAAVENAESGKGATVFGYYVDDPERFGIVEFDKEGKAISI
                     EEKPEHPKSNYCVTGLYFYDNNVVEYAK"
     assembly_gap    6301..6453
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6454..6603
                     /locus_tag="DHV81_06725"
     CDS             <6454..6603
                     /locus_tag="DHV81_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HCI94154.1"
                     /translation="KIACLEEIAYLNGWITKDEVLKTYELVKKNQYGQYLKDVLDGKY
                     REQLY"
     gene            6617..7660
                     /locus_tag="DHV81_06730"
                     /pseudo
     CDS             6617..7660
                     /locus_tag="DHV81_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529743.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
     assembly_gap    6895..7027
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7743..>8635
                     /gene="rfbD"
                     /locus_tag="DHV81_06735"
     CDS             7743..>8635
                     /gene="rfbD"
                     /locus_tag="DHV81_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCI94155.1"
                     /translation="MKVFVTGVAGQLGHDVMNELAARGYEGIGTDIAPQYSGADDGTA
                     VTKMPYVSLDITDRDAVEKTITELKPDVVVHCAAWTAVDLAEDDDKKAKVHAINADGT
                     QNIADACKKIDAKMVYISTDYVFDGQGTTPWQPDCKDYAPLNVYGQTKLDGELAVANT
                     LEKYFIVRIAWVFGVNGKNFIKTMLNVGKTHDRLTVVSDQIGTPTYTLDLSRLLVDMI
                     ETDKYGYYHATNEGGYISWYDFTKEIFKQAVELGHTEYSEDRLTVAPVTTAEYGVSKA
                     ARPFNSRLDKSKLVENGFKPLP"
BASE COUNT         2703 a         1481 c         2001 g         2164 t
ORIGIN      
        1 tgtaaaggag gacgtaggat accactttgt ttttcgataa aggcaaatcc gtttctactt
       61 cacaggctgc cggcgggctt agaccatgtg gaggtgtgca gcccgggaga gcttgagata
      121 tgtatagcac ttggcgtgaa gccggagcgc atcatctatt caggtgtgat gaaggaaaaa
      181 tgcgatatag agcgagcagt ttcatatggc gccgggatac tgacatgtga gagcataaga
      241 catgcggcgc tgatatcaga agtgatgctt gaatgtatac aggaaggtgc gtatgaagcc
      301 gagtttgccg aaacgaaagc acatgtcata ctgcgcctga caagtggcaa ccagtttggc
      361 atgtcgcttg aggatattga atatatcatt tcgcatccgg atgagtttaa gggcattgcg
      421 gtaatgggta ttcactacta ttccggcaca cagaagagtc ttcgcaaaat aaataaggac
      481 ttggaaaaaa taaagtctgc actgaccgga ttaaaagaaa aatacggctt tgagccacag
      541 cttgtagagt atggcccggg actttgcgtg gagtattttg aggatgactg gcaggaaaag
      601 gaaaaacaat cacttgatga ggcagcagag gtgcttagag aatttgctgt ggaatatccg
      661 cttggcattg agatgggacg attcctcgca gcctcatgcg gtaaatatta cacacaggta
      721 aaggacttaa aatcgacagg cgatgccaac tatgcgattc tggatggagg aatccaccac
      781 ctgaattact tcgggcagag gatggcaatg caggtgccac cgattagtat atatagagct
      841 gccggtgtgg aattaacaca attgccggac atagactata cactctgcgg aagcctctgt
      901 acagtggcag atgtgctggt acgcgaggtg aggcttaaga agcttaagct tggcgatgtt
      961 cttgagttcg ggcactgtgg cgcatactct gtgacagaag caccggcact ctttttgtca
     1021 agacagcttc cggctatata tgcatattcg aaggagtatg gatatgagtg cctgagggag
     1081 catattcctg cggctgagat aaatctggcc ggaaaaaaat tgtaaataaa agcgccatgg
     1141 taaattgcca tggcgctttt gaatatgaca taaacaaagc attttgtggg aagcctgact
     1201 attagccaag ctccttctca cacatatcaa gtacagaggc aatggtagta tccatatcat
     1261 agtatttgta ctcgccgaga cgaccaccga atataacaca ggtctcctta tcggcaagct
     1321 ccttatactt ggcgtagagc tgtccatttt tctcatcgtt tacagggtag taaggctcat
     1381 cgcctgcctt ccactcagaa gagtactcac ggctgataat agtctttggc agatcattgc
     1441 catcctcatc cttgccgaac tcgaaccact tgtgctctat gatacgtgtc catggagtct
     1501 cgcggtctgt atagttgaca gcggcattgc cctgaaagtt aggaatatcg agggtctcat
     1561 tctcaaagcg cactgagcgg tattccaaag tacctagctt aaagccaaag tatgcgtcaa
     1621 taggacctgt gtatacaacc ttgtcagcaa gagcatcaag ctcagccttg tgctcaaggt
     1681 agtcggtatt caatctgacc tcaatgccgt cgagcagatt ttcaaccatc ttggtgtatc
     1741 caccgattgg aataccctga taaagagcat tgaagtaatt gttgtcaaag gtaagtctaa
     1801 caggaagacg ctttatgatg aaagccggaa gctccttaca gtcacgcccc cactgcttct
     1861 cagtgtagcc cttaacgagc ttctcataga tatcacggcc aacgagtgag atagcctgct
     1921 cctcgagatt ttttggctca ccggttattt cctttttctg ctcctcaatc tttgcagcag
     1981 cctcctccgg tgtcaccaca ccccacatct tgttgaaggt gtacatgtta aatggcatag
     2041 agtaaagctc acccttgtag tttgccacag ggctgtttgt gaaacggttg aactcagcaa
     2101 actgtgtgat gtagttccag accctcttgt cattggtgtg gaatatgtgg gctccgtaca
     2161 tgtgtacatt tatgccctcc tgcttttcgg tgtaaacatt tcctgctatg ttaggacgct
     2221 tgtccacaac aagcactgat ttgcccttgg actttgcctc atgggcgaag atggatccgt
     2281 agagaccgga acctactatt aaataatcgt atttcattgt gttctcctac ttaatttgcg
     2341 ttatataaat tattataagt gttgattaaa aaaaagcaag agataaaaca tttttacgta
     2401 caaataaata catattgtat ttattttggt gatataatgt gttatagtga ataaaaagga
     2461 ggatacaaag atgaagaata tgtatttggt taaatattcg gttaaaggaa taaaatcgtt
     2521 ggataagctg gtgtcattgg ctttttacaa aaaaacaatt acaaaagaca tggatacgca
     2581 agggtttaat ataaaaggaa tatacggaat gaatggttcg ggaaaatcag gaatcatgtc
     2641 atcaattcag attttaaaaa atatcttaac agattctaac tatttaaata atccaataag
     2701 ccagaaaacg ctggattcac ttgtgaataa gaaattatct gagttgttta ttgaagtgga
     2761 atatgtagta agaacaaagg aaaaattgtg gttatttcga tataatgtag cattagcaaa
     2821 aaaagaatcg gaaacatata ttataaaaag tgaagagctt tcatttaaaa atgcaacaac
     2881 aagaaaagaa aatatgagtg ttttgtacaa agtaaatgat ggagcattgg atttaaaata
     2941 ttttgataca agtgataaga caaatagtat gttagcagaa aaaacaataa atctattaga
     3001 taaatcatca ttttgctcaa ttgctgtaaa aaaaattata gataaaaaaa tatttgataa
     3061 aaaggttcaa atggttgttc cggctttggt tattttatat aattttggaa caaagctaca
     3121 tgtatatctt gacaatgagg atactcatat accatttata attgcacata tgatgaagta
     3181 cggagatata acatatgaag gcaatgaaat ggtagatagc ctgttgtatg aggcttcaaa
     3241 tatggatgca gagagtatga atatgttagc tgcggagaaa aattttgtta acagggatag
     3301 ttatgattat tttgaaaatg aagttcatca gttgtacgag tttttacaca tttttaagtc
     3361 ggatttggtg gggattgaaa tagaaaaaaa ggaagatgga gacatgtatg tctgtgagct
     3421 tgtaatggtt tacaatgaat atagggttaa cgtggagttt gaaagtacag gcataaagaa
     3481 gctggtgaag ctttatatgt atattagaga gatgaaacga ggaggaatcg tatttataga
     3541 tgaatttgat gccaatcttc atgatgtcta tctgtgcgct ttactggaat atttaatgga
     3601 gtatggcgag gggcaactgt gttttacgac acacaatata ggaccgatgg atattctcaa
     3661 aagaaatagt aattcaattg attttatttc gggagaccat aggatttatt cgtggaaaaa
     3721 atcagggaat tattcaccgg ctaatcttta taggaaaggg atgatagagg gatctccgtt
     3781 caatgtggat tctatagatt ttatagatgc atttggtata caggaggggg atgaataatg
     3841 ggaatgcagc taatatttgt tttagagaca aataagaaat gtaaatcgga ttggatatat
     3901 atcaaggata ctattgaata tttttataat atagatgcca ataatataaa gtttacacag
     3961 gtgtatatgg atggtaaggg gaatttcaag aataaacaaa aggaaattaa taaaaatata
     4021 gaccagtatg ctaaaggtgc aagtaatggt aactccaaag ttatttgttg cttggatagt
     4081 gatgattatg atatcaacat ggaggcaatt aaattcttga acgaggcttt caagttttgc
     4141 aatgataata aatatgaatt aatatggttt tgtaaggata ttgagcgtgt ttatctaaga
     4201 aagaaggttg tgaaagatga aaagaaaaag gaggcaggga aatttaaagc aaataaaact
     4261 attagaaata ttgatgcaaa aaatttagtt gctagcaagt ataaggatgg aacaagcaat
     4321 atattgaaca ttcttgacaa atatttggag cgaaagtaat ggaggtataa aagtgaaact
     4381 acccggctac tactcctcag gagaatttgc aaaaaaagcg catattacaa aaaagaccgt
     4441 caggtactat gacgagcaca atattttaaa accatcctac gtaaacgaaa acggagccag
     4501 attctataat gatgacgact ttgcgcgtct acagcagata ctgttcctca aatatctggg
     4561 attttccctg gatgatatca aggagatgac actcagaaac acggacagta atgtcatgtc
     4621 tgagtcgctt cacatgcagc ttggcctcgt ggaggagagg atagagcaga tgaagcttat
     4681 gaaatcggca ttgcaggagg cttcagaggt aatagacgag gggcgaagcg tggactggag
     4741 ccatatgctg gagctggtca atataaacga gatggagcaa aagctaaaac agcagtacag
     4801 ggatgcttca aatatatcag ccagaatcaa tctgcacagg gatttctcaa tgaatccggt
     4861 gagctggttt agctgggtat ttgatgagtg tagctttttt gagggggaaa aggtgctcga
     4921 ggtgggctgc ggtgatgcct ctctctggac acagaacata gacaggatac cggcagatat
     4981 gcagataacc ctgactgata tatcgtatgg catggtaagg gatgcgacga gaaatgtagg
     5041 cgcagatgac aagcgcttca catatgaggt gatggatgcc cacaggctgt ataagccgga
     5101 tgcatcgtat gactgcgtga tagcagacca tgtactgttc tattgtgaca atctggatgc
     5161 cgtgtgcagt gaaatcctaa gggtgctaaa gccaggtggc gtgtttgtgt gcagtacata
     5221 cagctcacgg catatgaagg aaataaacga cctggtgcag cagtttgatg accgtataga
     5281 gctgtcagcg gaaaggctgt atgagcaatt tggtaaagaa aatggtgagg caatacttgg
     5341 aaaatacttt ggtgaggtaa gctggcatgg atacgaggat gagcttgtgg tagaaaaagc
     5401 agagcctgtg atagagtatg tgctctcgtg ccacggcaac cagaacaggt atattgtgga
     5461 ccgctataag gaatttgcgg catttgtcag aaagaagaca gagggagggt tccatatcac
     5521 aaaggatgca ggtgtattta ttgcacgaaa atgattcaaa aaacaggata ttataagcat
     5581 atattttgtg aaaaatgcta aaaatataaa aaagtacttg aaggtgccct tggggcacct
     5641 tttataatgc aggtaaatga tgaatcaggt cagtggattt tacagaccga aagcatcaaa
     5701 tgcagcacgt agtttcagtt tgaaggagaa caacatgaaa ggtattatat tagcgggagg
     5761 ttcaggcaca cgtctttacc cactcacaat ggtaacatca aagcagttac ttccaattta
     5821 tgacaagcca atgatctact atcctatgtc agtgcttatg aatgcaggca tcagagatat
     5881 tctgatcatt tcaacaccac aggatacacc aagattcaag gagctcttag gagacggaca
     5941 tcagtttggt gtcaacctca cctacgaggt acagccaagc cctgacggac ttgcacaggc
     6001 atttgttatc ggtgagaaat tcatcggtga tgatacagta gccatggttc tcggagacaa
     6061 catcttcgca ggacacggtc tcaacaagag actcaaggct gcagttgaaa acgcagagag
     6121 tggtaagggt gccacagtat tcggatacta tgtagatgac cctgagagat tcggtatcgt
     6181 agagttcgat aaagagggaa aggctatttc aatcgaggaa aagccggagc atccaaagag
     6241 caattactgt gtcacaggtc tttacttcta cgacaacaat gtagtagagt acgcaaagaa
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagatag catgtcttga ggaaatcgct
     6481 tacttaaacg gctggattac aaaggatgaa gtattaaaga catatgagct cgtaaagaag
     6541 aaccagtacg gacagtactt aaaggatgta cttgatggaa aataccgcga gcagttatat
     6601 taaggaggaa atcaaaatga caattatcgt aacaggtgga gcaggattta tcggaagcaa
     6661 cttcgtattt catatgctta acaaatatgc ggattatcgt atcgtatgct tagactgtct
     6721 cacatatgca ggaaatctct caacacttga gcctgtaatg gacaatccaa acttccgttt
     6781 cgtaaaggag agcatcacag accgtgacgc cgtatacaaa ttatttgagg aagagcatcc
     6841 tgatatggtt gtaaacttcg cagcagagtc acacgttgac cgttcaatcg agaannnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnngga gatctgccac ttgaccgccc tgaccttttc ttccatgagg atacaccaat
     7081 ccatacatca agcccatatt catcatcaaa ggcagcagca gacttactcg tgcttgctta
     7141 ccaccgcaca tatggacttc ctgtaacaat cagccgttgc tcaaacaact acggaccata
     7201 ccatttcccg gagaagctta tccctcttat gatagcaaat gcacttaatg ataagccact
     7261 cccggtatac ggaacaggcg agaacgtgcg tgactggctc tatgtagagg atcactgcaa
     7321 ggcaatcgac cttatcatcc acaacggacg tgtaggagag gtttacaacg taggtggaca
     7381 caatgagaag acaaaccttg aaatcgtaaa gattatctgt aaggagctca acaagcctga
     7441 gagccttatc acattcgtag gagaccgtaa gggccatgat atgcgttacg caatcgaccc
     7501 aacaaagatc cacaatgagc tcggatggct tcctgagacc aagtttgagg acggaatcaa
     7561 gaagacaatc aaatggtacc ttgacaatcg tgagtggtgg gagacaatca tcagtggaga
     7621 gtaccagaac tactatgaga agatgtacgc gaatagatag ttattatcgt ttgtctggtg
     7681 gttttgcgca tatatggtga tgttagtcac gagataataa ttaagcataa aggagaccaa
     7741 aaatgaaagt atttgttaca ggagttgcag gtcagctcgg acacgatgta atgaacgagc
     7801 ttgcagcacg cggttacgaa ggaatcggaa cagatatagc accacagtac agcggagcag
     7861 acgatggcac agcagttaca aagatgccat atgtctcact cgacatcaca gaccgtgatg
     7921 cagtggaaaa aacaatcaca gagcttaagc ctgacgtggt agtacactgt gcagcatgga
     7981 cagcagtaga tctggccgag gatgatgata aaaaagcaaa ggtgcatgca atcaacgcag
     8041 acggcactca gaatatcgcg gatgcctgca agaagataga tgccaagatg gtatatatca
     8101 gcacagacta tgtatttgac ggacagggaa ccacaccatg gcagcctgac tgcaaggact
     8161 atgcaccgct caacgtatac ggacagacaa agctcgatgg agagcttgca gtggcaaata
     8221 ctcttgagaa gtactttatc gtgcgtatag catgggtatt tggagtgaac ggcaagaact
     8281 tcatcaagac aatgctaaat gtcggcaaga cacatgacag gctcacagta gtaagtgacc
     8341 agattggtac accgacatac acactcgacc tttcaagact gctcgtagat atgatagaga
     8401 ctgataaata cggttactat catgctacaa acgagggcgg atatatcagc tggtatgatt
     8461 tcacaaagga aatttttaag caggcagtgg agcttgggca tacagagtat tctgaggaca
     8521 gactcacagt cgcacctgtt acaacagcag agtatggtgt gtcgaaggca gcacgtccgt
     8581 ttaacagcag actggacaag agcaagctcg tagagaatgg atttaagccg ctccc
//
LOCUS       DPUF01000188            3622 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_25300, whole
            genome shotgun sequence.
ACCESSION   DPUF01000188 DPUF01000000
VERSION     DPUF01000188.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_25300"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="DHV81_06740"
     CDS             <1..209
                     /locus_tag="DHV81_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94156.1"
                     /translation="EDNLDKLEVYALYDSGRNDCTGSISADGSSLSFPDYGVYDMHVR
                     ITCKNGTRDHVRISIPINRKVADI"
     gene            211..1071
                     /locus_tag="DHV81_06745"
     CDS             211..1071
                     /locus_tag="DHV81_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94157.1"
                     /translation="MKNEKQHRLLFIIPAVTIAIILIICIAVAVSSKSGEKGKKKDSR
                     SVSVTENTQDIVAESETLELVKKDALKETNVVDKDNKQNNGMQSDINDNSAPVAGNSD
                     NANTASDKSSDKSSEDKTAADKKTSTGSNNTQQPAGNDSKPAASNQTAASEPAQSEPA
                     QPEAPKNEYVAPGWIFYGSKAGNGMSGEQKAYIDSLVKQWTDGSISDSYLAEQIAHKM
                     KEEWGLSLNSVGISGDDRCIYPSKNEIPNYNEALAESYGFYNFTGLYTKGEYDEYGYL
                     ICYMWGVGIF"
     gene            1074..2807
                     /locus_tag="DHV81_06750"
     CDS             1074..2807
                     /locus_tag="DHV81_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94158.1"
                     /translation="MKEFLKKLIVGVLLIICIGLNFEKSNIIADEWSESVKFYNSYGN
                     SAVFKATNIYEGDIYFCSAGNTSTSGTKYRTIGYKMSVKNSSGNVIQTIYYQMGGSYM
                     TLKCQAKSGGKEYMMYQIPLRYVKNRMNQESLNALSSGSCTIILDACMVLRVNGKNKG
                     GMNDNGPSWGKVYTTYAGISKAANWTDSALSALYSYYGKTVRGLFHTIDVRKSTGISS
                     VSGGGTYCYGTYVTISASSSTGYDFTNWNNDSSMSSSSYGFYVNSGGTYTAYAKAGTI
                     AVTFWRNTSASDSEKTSKSYTYGDINQAFPAVGWQMAGYHMNGWGNNSYDTIAGYPLL
                     CGVENSWIESNRPSKNIYAVWQENEYTIEYDTGVSATVKYSDTVTLPSQHMCIGWLLG
                     EEYPDIKYAPGESVQVADLCRILGIEYTDKAVIRMYALWEHEPTIEADDMFFSIKQAR
                     NGGITEQLIGSLISATDVEDGDIAFGDNEINYLKVKNFDDRKIESARDKDIIEIVLEA
                     KDSYGNITQKTISITFTDTQVKERTKAFGKIRFISEKYYGKNKAGGLMENSRWLNDPE
                     FNSLLRQALAI"
     gene            2912..3310
                     /locus_tag="DHV81_06755"
     CDS             2912..3310
                     /locus_tag="DHV81_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94159.1"
                     /translation="MKNVVLGIIGCLIVVYTAMLGLSVYNMTVRENQMEKSLSLALSG
                     AMNRYYEPNMYIVDKKPVSSYDVEKAVIEDINERLTAGGTAIATVYVCDMEKGIISAG
                     IEEEFKLPTGVTKKISCKKTIVADQPMEDN"
     gene            complement(3395..>3622)
                     /locus_tag="DHV81_06760"
     CDS             complement(3395..>3622)
                     /locus_tag="DHV81_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HCI94160.1"
                     /translation="GSANLEDVSDELGLHLESDDYDTIGGYCLEKLDHLPEKNEIIIT
                     DDNILLRIEAVDKNRIEKVYIKLPKPAEEEN"
BASE COUNT         1208 a          576 c          889 g          949 t
ORIGIN      
        1 atgaggataa tctggataag cttgaagttt atgccctgta tgattcgggc agaaatgact
       61 gtacaggcag catatcagcc gatggctcgt cacttagctt tccggattat ggtgtgtatg
      121 atatgcatgt caggataacc tgcaaaaacg gcacaaggga tcatgtgagg ataagtattc
      181 caattaatag aaaggtagcg gatatataaa atgaaaaatg aaaaacaaca cagactttta
      241 ttcattatac cggcagtgac aatagcaata attcttatca tatgtatagc agtggcagta
      301 agctcaaaat caggagaaaa ggggaagaaa aaggattccc ggtctgtcag tgtgactgaa
      361 aatacacagg atattgtagc agaaagcgag acgcttgagc ttgtgaaaaa ggatgcattg
      421 aaggaaacaa atgtagttga taaagataat aagcaaaata acggtatgca atcagatatc
      481 aatgataatt ccgcaccggt agccggtaat tcagataatg caaatacagc gtcagataaa
      541 tcttctgaca aatcatcaga agacaaaaca gcagcagata aaaagacatc gacaggcagc
      601 aataacacac aacaacctgc aggtaacgat tcaaaaccgg cagcatcaaa tcagacagcc
      661 gcatctgagc cggctcaaag tgagcccgcg cagccggaag caccaaaaaa tgagtatgtt
      721 gcaccgggat ggatatttta tggcagtaag gctggaaatg gcatgagtgg ggagcagaag
      781 gcgtatattg attcgttggt gaaacagtgg acagatggaa gtatatctga ttcatattta
      841 gcggaacaga ttgcacataa gatgaaagaa gagtggggat tatcattgaa ttcagtagga
      901 atttctggtg atgacagatg tatttatccg tcaaaaaatg agatacctaa ttataatgag
      961 gctctagctg agtcatatgg attttataat tttacaggcc tgtatactaa aggagaatat
     1021 gatgaatacg gatatctgat atgctatatg tggggagttg gtatttttta gttatgaaag
     1081 aatttttaaa gaagttaata gtaggagtat tgctaattat atgtataggg ttaaattttg
     1141 agaaaagtaa tattattgca gatgaatgga gtgagtcggt taagttttat aatagttacg
     1201 gaaacagtgc agtttttaag gctacaaaca tatatgaggg cgatatttat ttttgttcag
     1261 caggaaatac atctacttca ggaacaaaat ataggacaat aggctacaaa atgagtgtaa
     1321 aaaatagtag tgggaatgtt attcagacca tttattatca aatgggtgga agctacatga
     1381 cacttaaatg tcaagcaaag tcaggtggga aggaatatat gatgtaccaa attcctttga
     1441 gatatgttaa aaacagaatg aatcaagaat ccctaaatgc ccttagctcc ggctcctgca
     1501 ccatcatcct cgatgcctgc atggtgcttc gagtaaatgg caagaacaag ggtggcatga
     1561 atgataatgg tccttcctgg ggaaaggttt atactacata tgcaggtata tccaaagcgg
     1621 caaactggac ggacagcgca ctctcagcac tgtattcgta ttatggcaag acggtgagag
     1681 gactgtttca tacaattgat gtcaggaagt ctacaggcat aagcagtgta tcagggggag
     1741 gtacatactg ctatggtaca tatgtgacaa tcagtgcaag cagttctacc ggatatgatt
     1801 tcacgaattg gaacaatgat tcgtcaatga gctcatcatc atatggtttt tatgtaaatt
     1861 cgggtggtac ttatacggca tacgcaaagg caggtactat agctgtgaca ttctggcgga
     1921 atacgagtgc gtctgattca gaaaagacct caaagagcta cacatatgga gacatcaatc
     1981 aggcttttcc ggctgtgggc tggcagatgg ccgggtatca tatgaatggc tggggaaata
     2041 attcgtatga tacgattgcc ggatatccgt tattgtgtgg tgtggaaaac agctggattg
     2101 agtcaaacag accgtcgaaa aatatatacg cagtatggca ggaaaacgag tataccatag
     2161 aatatgatac cggtgtatct gccactgtga agtatagtga tacggtgaca ttgccaagcc
     2221 agcatatgtg tataggctgg cttttaggag aagagtatcc tgatataaaa tatgcgccgg
     2281 gagagagtgt acaggtagct gatttatgca ggatactggg gatagagtat acagataagg
     2341 cggtaatacg tatgtatgca ctttgggagc atgagcctac catagaggcg gatgatatgt
     2401 tcttttcgat taagcaggca aggaatggcg gtataacaga gcagctgata ggaagcctta
     2461 tatctgcaac ggatgtggag gatggtgata ttgcatttgg cgataacgag ataaattatc
     2521 tgaaagtgaa aaattttgat gaccgtaaaa tagaaagtgc acgtgacaag gatatcatag
     2581 agatagtgct tgaggcaaag gacagctatg gaaatatcac gcaaaagact atatccatca
     2641 catttacaga tactcaggtc aaggagagaa ccaaagcctt tggaaagatt agattcataa
     2701 gcgaaaagta ttatggcaaa aataaagcag gaggcctcat ggagaactcc cgctggttaa
     2761 acgatcctga atttaattct cttcttcggc aggccttggc aatttgatat agacctttca
     2821 gattcggttt ttatcaacag cctctatgat ggcacaaagg atcatgtgag gataagcgtt
     2881 ccaattaata gaaaggtagc aggcgtatag aatgaaaaat gtagtacttg gaataatagg
     2941 ctgtcttatt gtagtgtata cagcgatgct tgggcttagt gtatacaata tgacagtcag
     3001 ggaaaaccag atggaaaaga gtctgtcatt agcactttcg ggtgctatga acagatatta
     3061 cgagccgaat atgtatatcg tggataaaaa gcccgtcagc agctatgatg tggaaaaagc
     3121 tgtcatagag gacattaatg agaggcttac agcaggcggt actgcaattg ccacagtcta
     3181 tgtatgtgat atggaaaagg gcattatttc agcaggaata gaggaagagt tcaagcttcc
     3241 tacaggtgtg acaaagaaaa taagctgcaa aaagactatt gtagcagatc agccaatgga
     3301 ggataactag cgtatgaaga atggtatttg tattacagca aaaataaagc aggaggtctc
     3361 atggagaact cccgctggtt aaacgatcct gaatttaatt ctcttcttcg gcaggttttg
     3421 gcaatttgat atagaccttt tcgattcggt ttttatcaac agcctctatg cggagaagaa
     3481 tattgtcatc agtgatgatt atttcatttt tttcaggcag gtggtcaagc ttttcaaggc
     3541 agtagccacc gattgtgtca tagtcgtctg attcgaggtg cagaccaagc tcatcgctta
     3601 catcctcgag atttgcagag cc
//
LOCUS       DPUF01000189            5135 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_21000, whole
            genome shotgun sequence.
ACCESSION   DPUF01000189 DPUF01000000
VERSION     DPUF01000189.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_21000"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(107..337)
                     /locus_tag="DHV81_06765"
     CDS             complement(107..337)
                     /locus_tag="DHV81_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCI94161.1"
                     /translation="MRDTILEILEELRPDVDFEKETKLIDDKILESFDIVSLVSELTD
                     EFDITIRPKDLVPENFNSVDAIEKLVKELVEE"
     gene            complement(339..1928)
                     /locus_tag="DHV81_06770"
     CDS             complement(339..1928)
                     /locus_tag="DHV81_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation protein"
                     /protein_id="HCI94162.1"
                     /translation="MKNVLEWLEATVEKYPDKPAFSDTECSITFAQVYDIARNTGAYL
                     IEQLGVDRTPVAVFAGRKMVTPAYFLGVVYAGRPYAPIDASLPDKRIEKILENLCPRA
                     IVADRESLEHVESIVDELAKAEGFDRPQIFIAENISRFEQVACADSNCKISESAGDAV
                     TDSENDMLATVRRHMSMTDPLYIIYTSGSTGNPKGVMTSHLSLMTYINAYCDVMHIEE
                     SDVLGNQSPLDYIAAIRDIYLPLKTGCSTAIIPKEYFMEPNALFDYMNDKKVSSVGWS
                     VSAFTILTSLGAFEEVGLKSLRKICFSGSVMPCRVLRKWQENLPEAHFVNQYGPTEAT
                     ASCTYYSVDHLVEEDEVLPIGKAYDNYRVFLIDEHGNEPAVGEQGEICVCGPILALGY
                     YNDPERTAAAFTLNPLNKAYPERMYRTGDYGRLDEDGILHFCGRMDRQIKHMGHRVEL
                     DEVEHAANVVDGVAESCVIYNKAKEVLILFYTGDCDRRSLALALRDELPGFMVPRKIK
                     KLEQLPKLPNGKYDMKKLEEM"
     gene            complement(2034..3008)
                     /locus_tag="DHV81_06775"
     CDS             complement(2034..3008)
                     /locus_tag="DHV81_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCI94163.1"
                     /translation="MEKNKISIVVPCFNEKEALPLFYNKITQVFDQMSQESETAGLYY
                     ELIIVDDGSMDGTLDVAKELAANDSGVKYISFSRNFGKEAAMYAGLQHAVGEYVAIMD
                     ADLQDPPEMLPKMYQVLNKEGYDAVGTRRVTRKGEPAIRSFFARKFYRLMSRISKANM
                     VDGARDYRLMNRKYVDALLSLKEYNRFSKGLFGWVGFKVKWLEFENVNRVAGETKWSF
                     WQLFLYSLDGIVAFSNAPLYMASIAGVFSFIVAIIAMLFIIIRRLVFGDPVAGWASTV
                     VIILFIGGIQLLSIGILGLYLSKLYLEAKDRPIYLIGESNIEKPTEKN"
     gene            complement(2990..3574)
                     /locus_tag="DHV81_06780"
     CDS             complement(2990..3574)
                     /locus_tag="DHV81_06780"
                     /inference="COORDINATES: protein motif:HMM:PF04892.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94164.1"
                     /translation="MSLKDYIIRDIQEKFKYLPSAVYLALICIAMYIFVCLLRYLFTM
                     REVSQADERRKKIVGKIWHMLFCGTFVAYMYTLIMIVYYSREPGSRTGVADMVLWSNW
                     GMTLQMHAYFIENIVLFIPFGLIFPLVFKNWMRWFTIPGGAFISAAIEYTQLVTGRGY
                     CQLDDFVTNTAGAAIGFFIYLILYAGWLVWKKIR"
     gene            complement(3909..>5135)
                     /gene="glmM"
                     /locus_tag="DHV81_06785"
     CDS             complement(3909..>5135)
                     /gene="glmM"
                     /locus_tag="DHV81_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCI94165.1"
                     /translation="KIVIGKDTRRSSYMFEYSLVSGLTASGADAYLLHVTTTPSVAYV
                     ARTDDFDCGIMISASHNPYYDNGIKLINSNGEKMDEETILLVEDYIDGKLRLFGQEWK
                     ELPYAHKDAIGCTVDYVAGRNRYMGYLISLGVYSFKGMKVGLDCANGSSWNMAKSIFE
                     ALGAKCYVINNEPNGLNINNNAGSTHIEGLQKYVVDNGLDVGFAYDGDADRCLCVDEL
                     GNVITGDHILYIYGKYMKERGKLDNNTVVTTVMSNFGLYKALDELGIGYAKTAVGDKY
                     VYEYMQQNGCRIGGEQSGHIIFSKYASTGDGILTSLKMMEVMMARKQKMSQLCEGLNI
                     YPQVLQNVRVADKAEALANPEVQAAVDQVTAELGDTGRILVRESGTEPVIRVMVEAES
                     EEICQKYVDMVVAKLK"
BASE COUNT         1360 a         1340 c          892 g         1543 t
ORIGIN      
        1 ggtatcctac gtcctccttt acaatcaaga gcagccctta tcatggctgc tctcttcgca
       61 aactcgtccg tatcaaatac atagtacggt gtctgtctca tatgctttat tcctccacaa
      121 gctctttcac aagcttctcg attgcatcta ctgagttgaa attctcagga accagatcct
      181 ttggtctgat tgtgatgtca aactcatcag tcagctccga tacgagtgaa actatatcaa
      241 atgactcaag aattttgtca tcgatgagct ttgtctcctt ctcaaaatcc acatccggtc
      301 ttaattcctc taaaatctct aaaattgtgt ctctcatttt acatttcctc cagttttttc
      361 atatcatatt ttccgtttgg aagcttagga agctgctcca gtttcttgat tttccttggc
      421 accataaagc ccgggagctc gtccctcagt gccagcgcaa ggcttctcct gtcacagtct
      481 cctgtgtaga aaagtatcag cacctctttt gctttattat atataacaca gctttctgcg
      541 actccatcga ccacattggc ggcatgctcc acctcatcaa gctccacacg gtgtcccatg
      601 tgctttatct ggcggtccat acgtccacag aaatgaagta tgccgtcctc atcgagcctt
      661 ccgtaatccc ctgtgcggta catccgctca ggataagcct tgttgagtgg attgagtgta
      721 aatgccgcag ctgtcctctc cggatcatta taataaccca gcgccaggat tggtccacat
      781 acgcatatct cgccctgttc tccgacagcc ggctcatttc catgctcatc tatcaggaaa
      841 accctgtaat tgtcataggc ttttcctata ggaagcacct cgtcctcttc caccagatga
      901 tccacactat aatacgtaca cgaagcagtt gcctctgtcg gcccatactg gttcacaaaa
      961 tgcgcttccg gcagattttc ctgccatttt ctgagcaccc tgcatggcat tactgagcct
     1021 gagaagcaga tttttctgag actcttaagc cctacttcct caaatgctcc aagcgatgtg
     1081 agaatggtaa atgccgaaac actccagccg acactgctga ccttcttatc attcatgtaa
     1141 tcaaacaggg catttggctc cataaaatac tcttttggaa taatcgctgt gctgcagccg
     1201 gtctttagcg gcagataaat gtccctgatt gccgcaatat agtcaagcgg cgactgattg
     1261 cccagcacat cactttcctc tatatgcatc acatcacagt aagcattgat gtatgtcata
     1321 agtgacagat gtgatgtcat gactcccttt ggattgccgg tggagcctga cgtgtagata
     1381 atgtacagcg ggtctgtcat ggacatatgc ctgcggactg ttgcgagcat atcgttctca
     1441 ctatccgtca cagcgtctcc tgctgattca ctgattttac aattactatc agcgcacgcc
     1501 acctgctcga agcgcgatat attctcagca ataaatatct gcggtctatc gaaaccttct
     1561 gcctttgcca gctcatccac tatgctctcc acatgctcaa ggctttccct gtcagccaca
     1621 atagcccttg gacacagatt ttcaagtatt ttttcaattc tcttgtcagg aagcgatgca
     1681 tcaatcggcg catacggtct gcctgcatat accactccca ggaagtatgc cggtgttacc
     1741 attttccttc cggcaaatac tgcaacaggg gttctgtcca ctccaagctg ttctatcaga
     1801 tatgctcctg tatttcttgc tatatcatac acctgcgcaa acgtaatgct gcattcagta
     1861 tccgaaaatg caggcttgtc agggtatttt tctactgtgg cctcaagcca ctccagtaca
     1921 tttttcatct tagtcctctt tcatgcatta ataaacgttg ccgaaaaaat tatagcatat
     1981 aacatccgaa aaaaatgtca tgtgctatcg aattaagaat tcttaagaaa gccttaattt
     2041 ttttctgtag gtttttctat atttgattct cctattaaat atatcggtct gtcctttgcc
     2101 tcgagatata gcttcgacag ataaagtccc aaaatgccta tgctaagcag ttgtatccca
     2161 cctataaaaa gtatgataac aacggtcgaa gcccagccgg ctaccggatc tccgaataca
     2221 agtctcctga taatgataaa gagcattgca attattgcga caataaagct gaacactccg
     2281 gctattgaag ccatgtatag cggagcgttg gaaaacgcca ctattccgtc aaggctatac
     2341 aaaaagagtt gccagaatga ccactttgtc tcacctgcca ccctgtttac attctcaaac
     2401 tcaagccact tcaccttaaa gcctacccag ccaaacaatc cctttgaaaa cctgttgtat
     2461 tccttaagcg aaagcagtgc atccacgtat ttcctgttca tcagcctgta gtcacgtgcc
     2521 ccatcaacca tatttgcctt tgatattctg ctcataagcc tgtaaaattt tctggcaaaa
     2581 aaagatctaa tcgctggttc acctttgcgt gtcactcgtc ttgtaccgac agcatcataa
     2641 ccctctttat taaggacctg atacatcttc ggaagcattt ccgggggatc ctgaagatct
     2701 gcatccatta ttgccacata ttcccccacc gcatgctgca agcctgcata catggctgcc
     2761 tccttgccaa aattgcgtga aaaagatatg tattttacac cggaatcatt tgcggcaagc
     2821 tctttcgcga catcgagcgt cccgtccatg gagccatcat ccactataat cagttcatag
     2881 tacaatcccg ctgtctcact ctcttgactc atctgatcaa acacctgagt tattttatta
     2941 taaaaaagag gcagagcctc tttctcgtta aagcatggta ccacaatgct tatcttattt
     3001 ttttccatac cagccaccct gcatataata tcaaataaat aaaaaatccg attgctgctc
     3061 ctgccgtatt tgtcacaaaa tcatcaagct gacaatatcc cctgccggta accagctgcg
     3121 tgtactcaat tgctgcgctt ataaaagcac caccaggtat tgtaaaccat ctcatccaat
     3181 ttttaaaaac caatggaaat ataagcccaa atggtataaa caatactata ttttctataa
     3241 aataagcgtg catctgaagt gtcatgcccc aattgctcca cagcaccata tcggcgacgc
     3301 cggttctgct tcccggctcc cttgaataat acactatcat tataagcgta tacatatagg
     3361 ccacaaaggt gccgcagaac agcatatgcc agattttgcc cactatcttt tttcttctct
     3421 catctgcctg tgatacctct ctcattgtga aaagatatcg cagcagacac acaaaaatat
     3481 acatcgcaat gcagattaaa gccaaatata ccgctgacgg aagatatttg aacttttcct
     3541 gtatgtctct tattatataa tcctttaaac tcaatgtcct tttttatgct ccttgtaggc
     3601 acgaatgaac tccagctgtc gttcatcgtc ctttttctgt tcgtcatcag acatatgact
     3661 cactttaata attatacaca atattatgcc aattacaata ataccggcac ataatataaa
     3721 aataattaag atttcaggca tagactattt cctccggcag aatttaatta ctattctctc
     3781 tacagattaa attatatcca cttaatttat cttaattcac tcatttatga accctcataa
     3841 cagcaaaagc tgcatgatat tctcacacag cttttgtata ttacgtgtat tatctttggc
     3901 attaaccttt attttaattt tgcaacgacc atatccacat atttctgaca gatttcctcg
     3961 ctctctgcct caaccatgac acggataaca ggctctgtac ccgactcacg cacaagtata
     4021 cgtcctgtat cgccaagctc tgcagtaacc tggtcgacag ccgcctgaac ctcaggattg
     4081 gcaagtgctt ccgccttgtc agctacgcgc acattttgaa gcacctgtgg atagatatta
     4141 agcccctcac agagctggct catcttctgt ttcctagcca tcatgacctc catcatctta
     4201 aggctggtaa ggataccatc acctgttgat gcatacttag agaagatgat atgtcctgac
     4261 tgctctccgc cgatgcggca gccgttctgc tgcatgtact catatacata tttgtcacct
     4321 actgcagtct ttgcatagcc gattccaagc tcatcaaggg ccttatagag tccaaagttt
     4381 gacataactg ttgtgacaac agtattgtta tcgagctttc ctctctcttt catgtacttt
     4441 ccatagatgt agaggatatg gtctccagtg attacattgc ccagttcatc tacacagaga
     4501 catctgtctg catctccatc gtatgcgaag cctacatcaa ggccattgtc caccacgtac
     4561 ttctgtaagc cctcaatatg tgtagagcca gcattattgt tgatgtttag accgtttggc
     4621 tcattattga tgacatagca ctttgctccg agtgcctcaa aaatagactt tgccatgttc
     4681 catgagcttc cgttggcaca gtcaagacct accttcatgc ccttgaatga gtagacacca
     4741 agcgatatga ggtatcccat gtaacggtta cggcctgcca cataatccac tgtacatccg
     4801 attgcatcct tatgtgcata aggcaactcc ttccactcct gaccaaacag gcgtagcttg
     4861 ccgtctatat agtcctcgac aaggagaatt gtctcctcat ccatcttctc tccattgctg
     4921 ttgataagct tgattccatt gtcatagtat ggattatggc ttgccgaaat catgatacca
     4981 cagtcaaaat catcggttct tgctacatat gccactgatg gagttgtggt aacgtgtaat
     5041 agatatgcat ctgctcccga tgctgtaagt cctgatacaa gtgaatactc aaacatgtag
     5101 cttgaacgtc ttgtatcctt tcctattact atctt
//
LOCUS       DPUF01000190            3513 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16748, whole
            genome shotgun sequence.
ACCESSION   DPUF01000190 DPUF01000000
VERSION     DPUF01000190.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16748"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="DHV81_06790"
     CDS             complement(<1..92)
                     /locus_tag="DHV81_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCI94166.1"
                     /translation="MIAIIDYGAGNLRSVQKALTAIGEESIVTR"
     gene            complement(94..1653)
                     /locus_tag="DHV81_06795"
     CDS             complement(94..1653)
                     /locus_tag="DHV81_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94167.1"
                     /translation="MDYRWRILMSDKSTENYLDNLLDSMNKLQDTDTKMSGADQDEFL
                     KKFEDELENETYDEYLSNFEKELERENEQAKGQALNVQTPAPDDKDASLDDMLTEFDR
                     RMKEQETQGDKQENKADNNLSDAVFNFTPADSGIEMMDANMPEMAENPKQEETPESII
                     TNEPQMVEEIGEPDLAGNASKDILDILGSDGLDNIGDLLEGKTGETGADIDDYASSQM
                     KVHETADAAVDAQDTEKKSLLKRIISFFTKEPQSDDVTDIQSTAEGAETDAKTLSDEN
                     KQILEALDADGELNEATDKPKKGKKPKKEKKPKKPKKEKKPKAPKEKKERIGWEKGPK
                     LPKGPVIVIWVFVMSIVVFVLICTFLLGNNSKVTDAQNTYNQAIADLGQNKADSIELY
                     TNAYSRLSGLKLSGDDKQLYNKLSVLASVSGKYDAYKSFSDSGYVTMAADSLVCAAGR
                     CAVNADNAKEYGCELQLEQLKNIISDTLSSQYGLSYDDAIALYNIDDRDDYTLALTKK
                     LNELGIKEETK"
     gene            complement(1699..2598)
                     /locus_tag="DHV81_06800"
     CDS             complement(1699..2598)
                     /locus_tag="DHV81_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheV"
                     /protein_id="HCI94168.1"
                     /translation="MDTNILLESGTNELEVLEFTLGDNHYGINVAKIREILQYMPVTP
                     VPNTHPSVEGIFMPRDTMITVINLKNCLNLPQTDEKGLFIITNFNKLNVAFHVDQVIG
                     IHRVSWENIIKPDETLTGEHGSTATGVIKMDDRLIVILDFEAIVASISPQTGLRVNDI
                     DEMEARDRSDATILIAEDSALLSKLITECLKKSGYTNLIVEENGQEAWDVIEAMQAKG
                     DITKHLDCIITDIEMPLMDGHRLTKLVKSDAELRDIPVIIFSSLVNEEMRKKGEQLGA
                     DAQLTKPEIGKLVEAIDKLIDKA"
     gene            complement(2642..3379)
                     /locus_tag="DHV81_06805"
     CDS             complement(2642..3379)
                     /locus_tag="DHV81_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCI94169.1"
                     /translation="MMGRNIKMAAVAFTAIITLTGCGTKLYDMTDAEQGLIANYSAYV
                     VAKHNIYQQDGMTNATEKRGDMEDADDTEKVDEASPDNSLSQDNKTSEAGNDKSSNSQ
                     KEPSATQEKKVSLAEATGYGSLEITYDGVNESDVYNEGSYYSITAGSGNTFAVMKFTL
                     KNPTDEDINVDMFGNARTYKATFPDGKEYTAEGTFLTYSLNTFQGTVKAHDAVDVVLL
                     FKIPQGTACDNIKMSVIKDNEKSLIEL"
BASE COUNT          858 a          877 c          580 g         1198 t
ORIGIN      
        1 tctctggtca caatactctc ctctcctata gctgtaagag ccttttgtac gcttcgcagg
       61 ttgcctgctc catagtcaat gattgctatc atatcattta gtctcctctt taatgcctaa
      121 ttcatttaat ttctttgtca gtgccagtgt atagtcatct ctatcatcta tattatacaa
      181 agcaatggca tcatcatatg ataagccata ctgactgctc agggtatcag aaataatgtt
      241 tttaagctgc tcaagctgca gctcacatcc gtattccttt gcattgtctg catttacagc
      301 acatcgtcct gccgcacata ctaaggaatc cgctgccatg gttacatagc ctgaatcgct
      361 aaagctctta taggcatcat acttacctga cactgatgca agcaccgaca gcttattgta
      421 caactgctta tcgtcacccg aaagcttcaa gccgctcagc ctgctgtagg catttgtata
      481 cagttctatt gaatcagctt tattctgtcc cagatcagct attgcctgat tgtacgtatt
      541 ctgagcatca gtcactttgg agttgtttcc gagcaaaaag gtgcatatga gaacaaacac
      601 aactatcgac ataacaaata cccagattac tataacaggt cccttaggaa gcttaggtcc
      661 cttctcccat ccaatgcgtt ccttcttttc ctttggagcc tttggctttt tttctttttt
      721 aggttttttt ggcttctttt cttttttagg ctttttgccc ttctttggct tatctgtggc
      781 ttcgtttagc tctccgtcag catccagcgc ttcaagtatc tgcttattct catctgagag
      841 tgtctttgcg tcagtttctg caccttctgc tgtgctctgt atatctgtga catcatctga
      901 ctgcggctct tttgtaaaaa atgatattat tctttttaaa aggctctttt tttcagtatc
      961 ttgtgcatca actgctgcat cagccgtctc gtgcactttc atctggctgc ttgcataatc
     1021 atctatatca gcccctgtct caccggtttt tccttcaagc agatcaccga tattatccag
     1081 accatcacta cccagtatgt caagtatatc ctttgatgca tttcctgcca aatccggctc
     1141 tcctatttct tcaaccatct gaggttcatt tgttattata ctctccggag tctcctcctg
     1201 tttgggattc tcagccattt ccggcatgtt tgcatccatc atctctatcc cagaatctgc
     1261 cggtgtaaaa ttaaataccg catcgcttag attattgtct gctttgtttt cctgtttatc
     1321 tccctgagtc tcctgttcct tcatcctgcg gtcaaactca gtgagcatat catcaagtga
     1381 agcatccttg tcatcaggag ccggagtctg cacattaaga gcctgaccct tggcctgctc
     1441 attttccctc tcaagttcct tttcaaagtt cgacagatac tcatcatatg tctcattttc
     1501 aagctcgtcc tcaaactttt tgaggaattc atcctgatca gccccagaca tcttcgtatc
     1561 tgtatcctgc agcttattca ttgaatccag caaattgtcc agatagtttt ctgtcgattt
     1621 atcgctcatt agtattctcc atctataatc cattaaaggg tgcagctaaa caagccacac
     1681 cccttgtatc taaatatatt atgctttatc aatcagcttg tcaatcgcct ctacaagctt
     1741 tccgatttca ggctttgtaa gctgtgcgtc tgctccaagc tgttcaccct ttttcctcat
     1801 ctcttcattt accagtgaag agaatatgat aaccggtata tctctaagct ctgcatctga
     1861 ttttacaagc tttgtgagtc tgtgtccatc catgagtggc atctcaatat cagttataat
     1921 gcagtcaaga tgctttgtaa tatcgccctt tgcctgcata gcctctatca catcccaggc
     1981 ctcctgacca ttctcttcca ctatgagatt ggtatatccc gacttcttaa ggcactcggt
     2041 gatgagcttt gacaataaag ccgaatcctc tgcgataaga attgtagcat cactcctatc
     2101 tcttgcctcc atctcatcga tatcattaac cctaagtcct gtctgtggac taatagaagc
     2161 aactatagcc tcaaaatcaa ggataactat aagcctgtca tccattttga taacacctgt
     2221 agcagtgctg ccatgctcgc ccgtcagtgt ctcgtcaggc tttatgatat tctcccacga
     2281 tacgcggtgt ataccgataa cctgatctac atgaaatgcc acattgagtt tgttaaaatt
     2341 cgtaatgata aataagccct tttcatccgt ctggggcaga ttaagacaat tctttaaatt
     2401 gataaccgta atcattgtgt cacgtggcat aaatattccc tcaacactcg gatgagtatt
     2461 cggaacagga gtgaccggca tatattgcaa aatctctctg atctttgcga cattgattcc
     2521 gtagtgattg tctcccaacg tgaattcaag cacttcaagc tcgtttgttc ctgactccaa
     2581 taatatgttc gtatccataa tacaacctcc acctcttagt aatctaagta tatcatatgt
     2641 tttataattc aatcaaactt ttttcgttat cttttataac cgacatcttg atattgtcac
     2701 atgccgtacc ctgaggtatc ttgaaaagca gcaccacatc aaccgcatcg tgcgccttta
     2761 cggttccctg aaaagtatta agggaatatg taaggaatgt gccctcagcc gtgtattcct
     2821 ttccatcggg gaaagttgct ttatatgtcc tggcattgcc aaacatatca acatttatat
     2881 cctcgtctgt cggattttta agagtgaatt tcataaccgc aaaagtatta ccgcttcctg
     2941 ccgttataga atagtatgag ccctcattgt acacatctga ctcgtttact ccatcatatg
     3001 taatttcaag gctgccgtag cctgttgcct cggcaagtga caccttcttt tcctgcgtgg
     3061 cagaaggctc tttctgcgaa ttgctgctct tatcatttcc agcttcagat gtcttattat
     3121 cctgcgaaag tgaattatca ggtgacgcct cgtccacttt ctcagtgtcg tcagcatcct
     3181 ccatatcccc acgtttctcc gtagcgttag tcatgccatc ctgctggtat atattgtgtt
     3241 ttgcaacaac atatgctgaa taattagcga taaggccttg ttccgcatca gtcatatcat
     3301 ataattttgt tccgcagcct gtcaacgtta taattgctgt aaatgccaca gctgccattt
     3361 ttatatttct tcccatcatt gcaataaacc cgttcttatt ctatgacttt tatcctaata
     3421 atatatatag taacacattt attgattgtt tgcaataaat aactcaagtt caaaccagaa
     3481 ttctacacca tccttgtgat ttatgacacc ata
//
LOCUS       DPUF01000191            3115 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16620, whole
            genome shotgun sequence.
ACCESSION   DPUF01000191 DPUF01000000
VERSION     DPUF01000191.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16620"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="DHV81_06810"
     CDS             complement(<1..215)
                     /locus_tag="DHV81_06810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94170.1"
                     /translation="MEEEIKRFKFIGLNDKASQEHLLQNTLILSGFYSSVPARLLFVA
                     VSRDFCYFLKSQIDACPHRISATKRIL"
     gene            complement(200..1237)
                     /locus_tag="DHV81_06815"
     CDS             complement(200..1237)
                     /locus_tag="DHV81_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94171.1"
                     /translation="MIDNYEHYITKNIKAFYKRRLFSPIVYIILLTVLWFAFSLGDIL
                     SPIHIDDSVSFEAAYKDSDRYVKTTLKKLYFTGYTMKDGNDIKGYYYYCMRDKHCSIV
                     LLAPSTCEEGLPSIDKLTVVGKIVKGKGTYTQFVNKLSKDLSWDSKGLSDTITGYYLN
                     EPEYHLKTTIFMFVFYFGTLIYAVISLIFYILCIRFPVLAPACQNLVVFGNPHKLLAE
                     AEEELATLPQLATEDMFITEHYFIMTSPYGNAIVPIKEILWIYKYSTLHKILWYHFSI
                     SYTLHISANKHLYIHCPKNTKSDIDGIIDYLAEANHDILVGFSEENRLKVQAVQGKPL
                     HIERLLAWKKK"
     assembly_gap    1283..1349
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1369..2118
                     /locus_tag="DHV81_06820"
     CDS             1369..2118
                     /locus_tag="DHV81_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HCI94172.1"
                     /translation="MEAYTSFARVYDMFMDNVPYEVWSRYIVEKLRANGIDSGYVVDL
                     GCGTGKLTTLLADAGYDMIGIDNSFDMLDMALEREDDRILYLMQDMREFEPGEKVSAV
                     VSACDSINYILEPEDLQAVFFCVSESLKDGGIFIFDINTPYKYEVLMADNTIAENRDE
                     GSFIWDNYYDADEKINEYDLTLFIKEQSEDEDDIYKKFEETHFQRCYEISELKELLEQ
                     SGLVFDAVYDAYTDDQVKCDSEKVTVIAHKA"
     gene            2142..3008
                     /locus_tag="DHV81_06825"
     CDS             2142..3008
                     /locus_tag="DHV81_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCI94173.1"
                     /translation="MSDYIVRATAADANIRAFAVTSREMVETAREDHMTTPVMTAALG
                     RLLSAGAMMGAMMKGDKDIITLQIQCSGPAKGLTVTADSHGNVKGFAMNPQVELPLNA
                     AGKLDVGGALDLGILTVIKDMGLKEPYSGQCELKTGEIAEDLTYYFATSEQIPSAVGL
                     GVLVDKDQSVKQAGGFIIQLMPFTPDEVVDRLEKKITEIDSVTQMLDRGLTPEQILEE
                     ILGDFGLEITDTTETRFHCDCSKERVSRALSTLSKKDLDSIIADGESIEVKCQFCNKA
                     YEFTVDELKEMR"
BASE COUNT          903 a          564 c          773 g          808 t
ORIGIN      
        1 ttcagtattc gtttagtggc agatatgcgg tgcggacagg catctatttg ggattttaaa
       61 aaatagcaaa aatctctgga cactgcaaca aacaggagcc tcgcaggaac actgctgtaa
      121 aatccgctta gtatgagcgt attttgcagc aagtgctcct gcgaggcttt gtcatttaat
      181 ccaataaact taaaacgctt tatttcttct tccacgcaag aagcctctca atatgcagcg
      241 gtttcccctg aacagcctgc accttcaatc tgttttcctc actgaaacca accagaatat
      301 catgattagc ctcagccaga taatcgatga taccatcgat atcagacttc gtgttcttcg
      361 gacaatgaat atacagatgc ttatttgcgg aaatatgcag tgtatatgag atgctgaaat
      421 gataccacag aatcttgtgc agggtagagt atttgtaaat ccataaaatc tctttgatag
      481 gcacgatggc attgccataa ggcgaggtca tgataaaata gtgctctgtg ataaacatgt
      541 cctcggtggc aagctgtggc agtgtcgcaa gctcctcctc agcctcggca agcagcttgt
      601 gcggattgcc aaatacaaca agattctgac atgccggtgc caaaacagga aacctgatgc
      661 ataggatgta gaatatcagg ctgataacag catagatgag agtgccaaaa taaaaaacaa
      721 acataaaaat agtagttttc aaatggtact caggctcatt aagatagtag cctgttatgg
      781 tatcggagag tcccttggaa tcccaactca gatccttaga cagtttattt acaaactgtg
      841 tgtaggtgcc ctttcccttt acgattttac cgaccaccgt gagtttgtcg atagagggca
      901 gaccctcctc acaggtggat ggtgcaagca gtacaataga gcagtgctta tcacgcatgc
      961 agtagtagta atagcccttg atatcgttgc cgtccttcat ggtgtagccg gtaaagtaaa
     1021 gctttttaag ggtggttttt acatatctgt ccgaatcctt gtatgcagct tcaaagctga
     1081 cagaatcgtc aatgtgtatg ggcgataaaa tatctccgag tgaaaatgca aaccatagca
     1141 cggtaagcag aatgatgtat acaatcggag agaaaagacg ccttttgtaa aatgctttga
     1201 tgttttttgt tatgtagtgt tcgtaattat caatcatatg cttagtataa tggtttttgt
     1261 gccttatatc aaccttgatt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna acacgaaatg aggtaattat ggaagcatac
     1381 acgagttttg cccgggtgta tgacatgttc atggacaatg tgccatatga ggtgtggagc
     1441 agatatattg tcgaaaaact gcgtgcaaat ggcatagaca gtggatatgt cgtggactta
     1501 ggctgtggta cgggaaagct taccacgctt cttgcagatg caggctatga tatgataggc
     1561 attgacaatt cttttgacat gcttgatatg gcacttgaga gagaggatga cagaatcctt
     1621 tatctgatgc aggatatgag agagtttgaa ccgggagaaa aggtgagtgc cgtcgtaagc
     1681 gcatgtgaca gcatcaatta cattcttgag cctgaggatc tgcaggcagt ttttttctgc
     1741 gtgagcgaaa gtctcaaaga tggtggcatt tttatatttg atatcaatac tccatataaa
     1801 tatgaggtgc tcatggctga taataccata gctgaaaacc gtgatgaggg cagctttata
     1861 tgggacaatt attatgatgc cgatgagaaa ataaacgagt atgacctgac actttttata
     1921 aaggagcagt cagaggatga ggatgacatc tacaagaagt ttgaggaaac tcatttccag
     1981 agatgctatg agatttcaga gcttaaggag cttcttgagc agtcgggact tgtgtttgat
     2041 gcggtgtatg atgcgtacac agacgatcag gtgaagtgtg acagtgagaa ggtcactgta
     2101 attgcacata aagcatagaa ttagataatg agaggtaaat tatgtcagat tatatagtaa
     2161 gagcaacagc ggcggatgcc aatatcagag catttgctgt aacatcaagg gagatggtgg
     2221 agaccgccag agaggatcac atgacaacac ctgtcatgac ggcggcactt ggcagacttc
     2281 tttctgcggg agccatgatg ggagctatga tgaagggcga taaggatatc ataactcttc
     2341 agatacagtg cagcggacct gcaaagggac ttaccgtcac agcagattcg cacggcaatg
     2401 taaagggctt tgccatgaat ccacaggttg agctgccact caatgcggca ggcaagctcg
     2461 atgtgggtgg agcgcttgat cttggaatac tcacagtcat aaaggatatg ggcttaaagg
     2521 agccgtattc aggtcagtgt gagcttaaaa caggtgagat agctgaggat ctgacctatt
     2581 attttgccac atctgagcag attccgtcgg cggttggtct cggcgtgctg gtggataagg
     2641 accagagcgt aaagcaggca ggcggattta tcatccagct tatgccgttt acaccggatg
     2701 aggttgtgga caggcttgaa aagaaaatca cagagattga ttctgtgaca cagatgcttg
     2761 acagggggct gactccggag cagatacttg aggaaatttt aggagatttc gggcttgaaa
     2821 tcacagatac gactgagaca cgcttccact gtgactgctc aaaggagcgt gtgagcagag
     2881 cactttccac actgagcaaa aaggatcttg attccattat cgcagatggt gagagcattg
     2941 aggtaaagtg ccagttctgt aataaggcat atgagtttac ggtggatgag ttgaaggaga
     3001 tgcgctaatt gaaggacgga tatataaagg tcgctgcagc gacaccaaag gtaaaggtag
     3061 ctgatactgt atacaacgga cagcttatta aagctcttat gaaggagtgc actga
//
LOCUS       DPUF01000192            3063 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_9439, whole
            genome shotgun sequence.
ACCESSION   DPUF01000192 DPUF01000000
VERSION     DPUF01000192.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_9439"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="DHV81_06830"
     CDS             <1..810
                     /locus_tag="DHV81_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HCI94174.1"
                     /translation="LDIMQKVQDVKKSSLTFAPEAGTQRLRNVINKGLTVDDIMNGSK
                     QAFEGGWNKVKFYFMLGLPTETEEDMRGIPELANDVAALYYDTVPKEKRAGKCQITIS
                     TSFFVPKPFTPFQWARMYEPSEYLGRAKIVNDHVKEQLNRKSIRYNWHEAYVTVIEGI
                     LARGDRRLCDAIVKVYEKGGYYDAWTEYFDYDRWIDSIKECGLDPDFYTMRERPLDEV
                     FPWDFIDIGVTKQFMIREWETAHKETVTPNCRMRCSACGAKSYGGGVCYEN"
     gene            800..1519
                     /locus_tag="DHV81_06835"
     CDS             800..1519
                     /locus_tag="DHV81_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HCI94175.1"
                     /translation="MKIRVKFKKWGCMKFIGHLDMMRYFQKAVRRADIDIRYSEGYSA
                     HQIMSFAAPLGVGITSDGEYFDIDVNTTESTEKSIQALNAQMVDGVEVTGYVLLPDDA
                     KKAMSLVAAADYVLSFKEGYESPYIIEEWKEAIDKHFFDAPSFVVMKKTKKSEREVDI
                     KPLAYKLTVMENNKKPEFFMQVSTGSIDNIKPEFVLQAIYEKCGLDYNPLAIQIHRQE
                     VYARKDDGTLICLLDMGEEIS"
     gene            1519..2727
                     /locus_tag="DHV81_06840"
     CDS             1519..2727
                     /locus_tag="DHV81_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HCI94176.1"
                     /translation="MNRIILTKTVKNNNTYTVYALMDENGNYKDFQLLPEKDTIINNI
                     YAARVNKILPGINAAFVTIAQNKSCYLSLNDAKNPVYTNKKSKKEGLCEGDEILVQVI
                     KDALKTKDPVVTTKLSIFGSNVILTNFDTQIGVSSKLDKERAALLRKAVLLSCADHEK
                     DGYGIIVRTNAKNVEDKVVSQDAYSVAQKYNQIIKKAPHQALYSCVYHGMSDYLLLMK
                     TIDFATVEWIKTDCDDIYDSLLTEYGIYDHAPEKIMRYDDSAISLSTLYGIRGLIDNL
                     TSRRVWLDCGGNIIIEQLETLTFIDVNSAKNISSGSNSILKTNMEAAKEIARQLRLRN
                     ISGMIIIDFINMKSEASKDTLIEALKRYIKDDNTVCTFVDITKLGLVELTRKKVHKSL
                     KQILEKTLDE"
     gene            2831..>3063
                     /gene="rplU"
                     /locus_tag="DHV81_06845"
     CDS             2831..>3063
                     /gene="rplU"
                     /locus_tag="DHV81_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCI94177.1"
                     /translation="MYAIIATGGKQYKVSEGDIITIEKLGVEAGEKVTFDQVLVVGGD
                     DLKVGDPTVAGATVEASVVKEGRAKKVIVYKYK"
BASE COUNT         1013 a          526 c          772 g          752 t
ORIGIN      
        1 ctcgatatca tgcagaaggt gcaggatgtc aaaaaaagca gtctgacctt tgcgcctgag
       61 gccggtacac agcgccttag aaatgtcatt aacaagggac ttaccgtgga tgatatcatg
      121 aacggctcaa aacaggcatt tgagggcggc tggaacaagg tgaagtttta tttcatgctc
      181 ggtctgccta cagagacaga ggaagatatg aggggcattc ctgagcttgc aaacgatgtg
      241 gcggcattgt actatgatac cgttccaaag gaaaagagag ccggaaaatg ccagattacg
      301 ataagtacat catttttcgt gccaaagcct tttacaccat tccagtgggc gagaatgtat
      361 gagccttctg agtatctggg cagagccaaa atagtaaatg accatgtaaa ggagcagctc
      421 aacagaaaga gcatcagata caactggcat gaggcctatg tcactgtgat agagggcata
      481 cttgcaagag gcgacagaag gctttgcgat gcgatagtaa aggtatacga aaagggcgga
      541 tattatgatg cctggacaga gtattttgac tatgacagat ggattgattc gataaaggaa
      601 tgcggactgg atccggattt ctacacgatg agagaaaggc ctttagatga agtattccca
      661 tgggatttta tcgatatagg tgtgacaaag cagtttatga taagagagtg ggagacagcc
      721 cataaggaga cagtcactcc taactgccgc atgaggtgct ctgcctgcgg tgcaaagagc
      781 tatggaggag gtgtctgcta tgaaaattag agtgaagttt aaaaaatggg gctgcatgaa
      841 gtttatagga catctcgata tgatgagata cttccagaag gcggtcagaa gggcagatat
      901 tgatatccgc tattcagagg ggtattctgc tcaccagatt atgtcgtttg cggctccgct
      961 tggagtgggc atcacaagtg atggtgagta ctttgacata gatgtaaata caacagaatc
     1021 tacagaaaaa agcatacaag ctttaaatgc ccagatggtg gacggagtag aggtgacagg
     1081 ctatgtgctt ctgccggatg atgcaaagaa ggctatgtcg cttgttgcgg ctgcagacta
     1141 tgtacttagc ttcaaggagg gctatgagag cccatacatc atagaggagt ggaaggaagc
     1201 gatagataag cacttttttg acgcgccatc ctttgttgtg atgaaaaaga ctaaaaagag
     1261 tgagagagag gtagatataa agcctcttgc atacaagctt acagtcatgg aaaataacaa
     1321 aaagcctgag tttttcatgc aggtatcaac aggaagcatt gacaatataa agcctgagtt
     1381 tgtgcttcag gccatatacg aaaagtgtgg acttgattac aatccacttg ccatacaaat
     1441 ccacaggcag gaagtgtatg caagaaaaga cgatggaaca ctcatttgtc tgcttgacat
     1501 gggagaagaa atctcataat gaacagaatt attctgacta aaacagtaaa aaataataat
     1561 acatacacag tgtatgcact gatggatgaa aacggaaatt ataaggattt ccagctcctt
     1621 cccgagaagg atacgataat aaacaatata tacgcggcaa gggtaaataa gatactgccg
     1681 ggcataaatg cggcatttgt gaccattgcg cagaataaaa gctgttatct gtcgctaaat
     1741 gatgcaaaaa atcctgtata cacaaacaaa aaatcaaaaa aagaaggatt gtgtgagggc
     1801 gatgagatac ttgtgcaggt cataaaggat gcgctaaaga caaaggatcc tgtggtcaca
     1861 acaaagcttt ctatattcgg aagcaatgtg attctcacca attttgatac acagataggt
     1921 gtttcatcga agctggataa ggaaagggcg gctcttttac gtaaggctgt gcttttaagt
     1981 tgtgcagatc acgaaaaaga tggctatggc atcatagtca gaaccaatgc caaaaatgtg
     2041 gaggataaag ttgtttcgca agatgcgtat tctgtagcac agaaatacaa tcagataata
     2101 aaaaaggcac cgcatcaggc gctctattca tgcgtatatc atgggatgag cgattatctg
     2161 cttttaatga agacaattga ttttgccacg gtggagtgga taaagactga ctgcgatgac
     2221 atatatgaca gtcttcttac agagtacggc atatatgacc atgctcctga aaagataatg
     2281 agatatgacg attcagctat cagtctgtca acactctatg gcataagagg gctgattgat
     2341 aatctgacct cgcgcagggt atggctggac tgtggtggca acatcataat agaacagctt
     2401 gagacactta cctttataga tgtaaacagt gcaaagaata tatcgtcggg gagcaatagc
     2461 atattaaaga ccaatatgga ggccgcaaag gagatagcaa ggcagcttag gctcagaaat
     2521 atctccggca tgataataat tgattttatc aatatgaagt ctgaggcgtc aaaggatacg
     2581 cttattgaag cgctcaaacg gtatataaag gacgataata cagtctgtac ctttgtggat
     2641 atcacgaagc ttggactggt ggagctgacc agaaaaaaag tccacaaatc tcttaaacaa
     2701 attttagaaa aaacacttga cgaatagata gctctatgct aatatactgg agtatgccgc
     2761 acagtgaggt ataagtttgc gactttcgca gcaccgtaac tggcgagtaa tgataaatag
     2821 gaggtgccat atgtacgcaa ttatagcaac aggtggtaag cagtacaaag tatctgaagg
     2881 cgatatcatt accattgaaa agcttggcgt tgaagctggt gagaaggtta cttttgatca
     2941 ggttttagtt gtaggcggag atgatctcaa ggttggagat ccaacagttg caggtgcaac
     3001 agttgaggct tctgtagtta aagaaggcag agctaaaaag gttatcgttt acaagtacaa
     3061 gag
//
LOCUS       DPUF01000193            8281 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_38413, whole
            genome shotgun sequence.
ACCESSION   DPUF01000193 DPUF01000000
VERSION     DPUF01000193.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_38413"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="DHV81_06850"
     CDS             complement(<1..791)
                     /locus_tag="DHV81_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCI94178.1"
                     /translation="MTHKKLIITEKPSVAQEFAKVLRVSGRNDGYIENGEYVISWCVG
                     HLVGLVYPESYDMKYKKWRLEDLPFLPKEYKYDVIDSVRKQYNIVHALLQREDIDRVY
                     WAGDAGKEGQTIEENIRNFGGVREGMEELRVWIDSQTEEEILRGIKEAKPMSAYANLG
                     KSGIMRTIEDYAMGINFSRVMSVKYGNLLNDAAGTRSYTAIAVGRVMTCVLGMVVIRE
                     REIRNFKETPFYRVIGRFTDAGVEAEWKAVEGSRYFESPLLYKENG"
     gene            879..2138
                     /locus_tag="DHV81_06855"
     CDS             879..2138
                     /locus_tag="DHV81_06855"
                     /inference="COORDINATES: protein motif:HMM:PF00589.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94179.1"
                     /translation="MISVINHLYQERRKIMEGINKVFTSELQTYVSELKEVMHQKGYS
                     DCQVQTYDKTWQSILSYASIQPNREFTEDFRQQFLQDVCAEALEKRDSMYRITRAINM
                     LSDFTQFHVIFRQYCTPKTDFSEELHEIFSEFLKAEEHRHFSEGTMKNLRGRLMRFHD
                     YLYDIGIRDFKSVTGSIINTYILSLARYSTTYVSESLREIRRMLTFGYENCFIETPLS
                     DFVPHVKNIRQQKIPSTFTDTEIEKILNSVDRSNPIGKRNYAIFLIAARLGIRSSDIR
                     ALTFSNINWDNKIISFCQQKTRHVLSLPLPDDVGWAIIDYLKNGRPETNVKNIFVSHM
                     YPYGELHSLGNVIPRQMRTAGINTPANKRTGMHAFRHGLATRMLENDVSLPVISQTLG
                     HADISSTEVYLRISIKQLALCGLEVDL"
     gene            2135..3124
                     /locus_tag="DHV81_06860"
     CDS             2135..3124
                     /locus_tag="DHV81_06860"
                     /inference="COORDINATES: protein motif:HMM:PF00589.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCI94180.1"
                     /translation="MKKRTVGSFDGPFAPMLDLFVKQKQALGYDYVGGYNALHVFDTF
                     SKRYEVKNYELSKEIVLAWAQKRLNENDTTRSARIMYLQHFAIFLNSQGYSGYIAPPQ
                     KYHFSQHTAYVFTKAEIKKLFISLDQMEYTPNSPYRHLSFPLLYRMLYGCGFRISELL
                     NLTVGDVDIERWIIHIHDSKNGNERLVPMAATLAARCRTYTESFHSDHEEDFPFFFKK
                     DGTGYCVSNIERHFREQLWLAGIPYLGKELGPRVHDLRHTFICHRLNQWAKEGVDLTA
                     MLPILSKYVGHTGIASTQYYLKLTAEAFPDVLEQMDELTGHVFPEVGGVVYEE"
     gene            3114..4133
                     /locus_tag="DHV81_06865"
     CDS             3114..4133
                     /locus_tag="DHV81_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94181.1"
                     /translation="MKNKIPDFAYHMTKFFTEYLPIQRNLSPNSIATYRDTFRLLLLF
                     YDTEKHIKPNLLTLDMLDVQGIEDFMLWLKKERNCGASTCNQRLGALKSFFRYLQFAL
                     PDRALQCQENLAIRPMKQSEPGLKYLTIDGIKLLLEQPDIKTKYGRRDLAILSLMYDT
                     GARVQEIADIKIVHIRFSAPATIRITGKGDKTRVVPLLSRTEDILKQYIKDFKIDAGG
                     NDAYLFQNRNGQHLSRFGISYILTKYADKARKVHPELIPERLSPHCIRHSKAMHLLQA
                     NVNLVYIRDLLGHSSVTTTEIYARADTTLKREALEKANPIKDTPAMPQWNDDEGLMEW
                     LRTLI"
     gene            4182..4454
                     /locus_tag="DHV81_06870"
     CDS             4182..4454
                     /locus_tag="DHV81_06870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94182.1"
                     /translation="MKNKSIDLVNEKTERKELLEWIPMLVKNKLNSLTTAQLRILHYI
                     LGYESREIDTEALMEEFEGYGYSEEQALEIAKILKRDNFLIMQKPY"
     gene            complement(4451..5218)
                     /locus_tag="DHV81_06875"
     CDS             complement(4451..5218)
                     /locus_tag="DHV81_06875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94183.1"
                     /translation="MAKYAYRVPNGTENDRVDASDLPLHGNGTFYCTTPDCRARMYVR
                     SPQKPSACFVSYNISEHTGGILCHIKDLFKPDDYCENLFSLDNFFTNLLTKQNRDSSP
                     HHGSGGKGSNSKIAINTLKMLYLMCVQYREGGSYNKYPIDDILVDKYNFDKYRENGII
                     GNRIVACTFWKYEPDTNCIFMNCPDERLYLPREEHKILKIHVTDDKMFEKCANKLKDK
                     THLHISVIGANWIPSNDDNCLAECEVVSVGKQICKGD"
     gene            complement(5384..6727)
                     /locus_tag="DHV81_06880"
     CDS             complement(5384..6727)
                     /locus_tag="DHV81_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCI94184.1"
                     /translation="MKAKHTDLEKLEQLYKESGNQLVVLYGRRGCQKEELIKEFCDGK
                     KFFYYRCRQASPEHQLEMMGEEISRQYKVSLSRQTYEEYFARIKSGDPSKLVVIIDEA
                     QFVAKRDTSFMEAVAKLKKHKLYPGPVLIILATSSTVWATQEAAEQFKGAEMKLGSLN
                     FLEVVRHFESLPVAEIVRIYGAIGGVPAYLDKWDAAKSFKDNICRLVLTSSGALYGEA
                     DAVISAELRELSAYSTILAAIARGENKLNDIFHATGFSRAKISVYLKNLANFNIVEKV
                     VSFETGGWENAKKGVYQIKDTFVNFWFKFVYPNMSNLYLLSPEEFYDTYIEKELDAYL
                     ERYFREVCTEYLLILNQMGQLPFTIKRVGTWIGKTGRIDIVAQSEDRRSIIGFCNWDK
                     PMMTMDMCENMAMAMDQARLTSDDYYLFSATDFEPALQSYVVRDPRFKLINMNEL"
     gene            complement(6801..8135)
                     /locus_tag="DHV81_06885"
     CDS             complement(6801..8135)
                     /locus_tag="DHV81_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI94185.1"
                     /translation="MVNGSIMDKLISFALPLMISGILQLAFNAVDIIVVGQFSGSKSL
                     AAVGSTTALINVFVNLFMGISLGANVLAARFYAAGKDDEMSKTVHTAIMIALISGIVM
                     GVVGVIFARGALEIMGTPDDVIGLSTIYMRIYFCGMPFFMLYNYGAAILRAVGDTKRP
                     LIFLIISGCINAMLNLFFVIVCKLGVAGVAIGTIVSQFVSCVLVLYCLSKADTSYKLC
                     FNKLAIDKEYMLQIFQVGIPAGIQSTVINISNAMLQSSVNSFGSIAMAGYTAANNILG
                     FLYVSVNSITQACMSFTSQNYAVGKQKRMDRVLIDCLFLTTAVALVMGSASYLFGTQI
                     LGIYTSNSEVIKSGLEIISITTIPYFLCGIMDLFPGALRGMGYSTVPMILSIIGTVGV
                     RIIWIYGLFPSHRSLFFLFISYPGSWIATIIMQAICYMFVRNKVKATIGARK"
BASE COUNT         2578 a         1800 c         1454 g         2449 t
ORIGIN      
        1 ccgttttcct tgtataaaag cggtgattca aagtaccttg agccttctac agccttccac
       61 tctgcctcta caccggcatc cgtaaacctg ccgattaccc tgtaaaacgg agtctcctta
      121 aagtttctga tttcgcgctc acggattact accattccaa gcacacaggt catcactcgt
      181 ccgaccgcga ttgccgtgta cgagcgtgta cccgctgcat cgtttagcag attgccgtat
      241 ttgaccgaca tgacacgcga aaagttgatg cccatcgcat aatcctcgat ggtacgcatg
      301 atgccgctct ttccgaggtt tgcatacgca ctcatcggct ttgcctcttt tatgcctctt
      361 aggatttcct cctcggtctg cgaatctatc cagactcgaa gctcctccat gccttccctg
      421 acaccaccaa agtttctgat attctcctcg atggtctgtc cttcttttcc ggcatcacct
      481 gcccagtaaa ctctgtcaat gtcctctctc tgcaaaagag cgtgcacaat attgtactgc
      541 ttgcgcacgc tgtctatcac atcatatttg tattcctttg gcagaaacgg cagatcctca
      601 agacgccatt tcttatattt catgtcgtag gactcagggt agaccagccc gaccagatgt
      661 cccacgcacc atgaaatcac atattctcca ttttctatat aaccgtcatt gcgtccgctc
      721 acccgcagca ccttcgcaaa ttcctgtgcc acggatggct tctctgttat tatcagtttt
      781 ttatgagtca tatattatgt taagtcagat gactctttcc tttctttatt agttttgcaa
      841 cgacaaatta ctcaacattt tctttccttt ttgataacat gattagtgta attaaccatc
      901 tatatcaaga aaggagaaag ataatggaag gaataaataa ggtttttaca tcagagttac
      961 aaacctatgt ttccgaatta aaggaggtga tgcaccaaaa ggggtatagt gattgtcaag
     1021 tacaaaccta tgataaaaca tggcaatcaa tactgtctta cgcttcaatt cagcccaatc
     1081 gagaattcac agaagatttt cgtcaacaat tccttcagga tgtatgtgcc gaagccttag
     1141 aaaagcgcga ttctatgtat cgcattacac gagcaataaa catgctttct gattttacac
     1201 agtttcacgt tatcttccgt caatattgta caccaaagac agatttttcg gaagagttac
     1261 atgaaatttt ttcagaattc ttaaaagctg aagaacatag acatttttcc gaaggtacaa
     1321 tgaaaaatct acgtggaagg cttatgcgat ttcatgatta tctttatgat attgggattc
     1381 gagattttaa gtctgtaaca ggctctatta taaacactta tattttatca cttgcaagat
     1441 attctacaac ttatgtctct gaatcactac gcgaaattcg caggatgtta acttttggat
     1501 acgaaaattg ctttattgaa actccacttt cagatttcgt tccacatgta aaaaatatcc
     1561 gccaacagaa aataccaagc acatttacag atacggagat tgaaaaaata ctcaattctg
     1621 tagaccgttc aaaccctatt ggcaaacgaa attatgctat tttcctgatt gcagcgagac
     1681 tagggattag aagtagcgac atccgagctt tgacattttc caatattaac tgggataata
     1741 aaatcatttc gttttgtcag caaaaaacca gacacgtact ttctttgcca ctaccagatg
     1801 acgtaggctg ggctatcata gattacttaa aaaatgggcg tccagaaact aatgttaaaa
     1861 acatttttgt gtctcatatg tatccttatg gagaattgca ttctcttggt aatgttattc
     1921 cacgacaaat gcgaacagcg ggcatcaata ctcccgctaa caaacgtact ggaatgcatg
     1981 cttttcgaca cggtttggct actagaatgc ttgaaaatga tgtttctctc cctgttatat
     2041 cgcaaacact tggacatgca gatatctcaa gcacagaagt ttacttgaga atcagtataa
     2101 agcaacttgc cctgtgtgga ttggaggtgg acttatgaag aaaagaacag ttggatcttt
     2161 tgatggacca tttgctccca tgttggattt gtttgtaaaa caaaagcagg cactaggata
     2221 tgattatgtg ggtggatata acgcattaca tgtatttgat acattcagca aacgatatga
     2281 agtgaaaaat tacgaattgt cgaaagaaat tgttctggct tgggcacaaa aacggctgaa
     2341 cgaaaatgat actacaagat cagcgaggat tatgtatctt caacactttg ctatattttt
     2401 aaacagccaa ggctactctg gctatatagc cccaccgcaa aaatatcatt tttcacaaca
     2461 tacggcatat gtattcacca aagcagagat taagaaacta tttatttctt tagatcaaat
     2521 ggaatatact ccaaattctc cataccgcca tctctcgttt ccgcttttat accggatgct
     2581 ttatggatgc ggtttccgca tatcggagct tcttaatctt acagttggcg atgttgatat
     2641 cgaacgttgg attattcata tccatgattc caaaaacgga aatgaacgac tcgttccaat
     2701 ggcagcaaca ctagcagctc gatgcagaac atatacagaa tcttttcaca gtgatcatga
     2761 agaagacttt ccattcttct tcaaaaaaga cggaaccgga tattgtgtaa gtaatataga
     2821 acgacatttt cgcgaacaac tatggctagc aggaatccca tatcttggca aagaactggg
     2881 accacgggtg catgatctac gtcacacatt tatatgtcat agattaaacc aatgggctaa
     2941 agaaggtgtt gatcttactg cgatgcttcc tattttgtct aaatacgttg gacatacagg
     3001 cattgcttcg acacagtatt atttaaaatt aaccgctgaa gcttttccag atgtgctaga
     3061 acaaatggac gagctaaccg ggcatgtatt tccagaagtc ggaggtgtgg tgtatgaaga
     3121 ataaaatacc tgattttgca tatcacatga ctaaattttt cacagaatac ttgccgatac
     3181 aacgaaatct aagtcctaat tcaatagcta cgtatcgtga cacatttcgg ttactgcttt
     3241 tgttttacga tacggaaaaa catataaaac ctaatttgct aacattagat atgcttgatg
     3301 ttcaaggcat tgaagatttt atgctatggc tcaaaaaaga aagaaattgt ggtgcaagta
     3361 cctgcaatca acgattaggc gcattaaaat catttttcag gtatctgcaa ttcgcactac
     3421 cagaccgtgc cctgcaatgt caggaaaatt tagccatacg gccaatgaag caatcagaac
     3481 ctgggttaaa atatcttact attgacggca taaaattact tcttgaacaa ccagatataa
     3541 aaaccaaata cggtcgccgg gatctggcta ttctcagtct tatgtacgac acaggtgcca
     3601 gagttcaaga aatcgcagat attaagatag ttcatattcg ttttagcgca cctgcaacaa
     3661 taagaataac cggaaaagga gataaaacac gcgttgttcc acttctttcc agaactgagg
     3721 atatattaaa gcaatatatt aaagatttta aaattgatgc aggcggaaat gatgcatacc
     3781 tatttcaaaa tagaaacggt caacatcttt cacgttttgg aatcagctat attttaacaa
     3841 aatacgcaga taaagcgagg aaagttcatc ctgaacttat tcccgaaaga ctttcgcctc
     3901 actgcatcag acattcaaaa gcaatgcatc tattacaagc aaatgtgaat ttagtctata
     3961 ttcgtgattt gcttggacac agtagtgtca ctacaacaga aatatatgcg agagcagata
     4021 caacgctcaa acgagaagct ctcgaaaaag caaatcctat taaggacact ccggcgatgc
     4081 cgcaatggaa tgatgatgag ggattgatgg agtggcttcg aactttaatc taaaacagtt
     4141 ttcgcgggtg atggagtatc acccgcagaa aggaataagt gatgaaaaat aaatcaattg
     4201 acttagtaaa cgagaaaaca gaacgaaaag aactgctcga atggatacct atgcttgtaa
     4261 aaaataaatt aaattctcta accactgctc aactccgtat cctacattat atattaggat
     4321 atgaaagccg tgagatagat acagaggcgt taatggaaga attcgaagga tatggctact
     4381 ccgaggaaca agcacttgaa attgcaaaaa ttttaaaaag ggataatttt ttaattatgc
     4441 aaaaaccgta ttagtcccct ttgcatattt gttttcccac agatacaact tcacactcag
     4501 ccagacaatt atcatcattt gacggtatcc aatttgcacc tattactgat atatgcaaat
     4561 gcgttttatc ttttagtttg ttcgcacact tttcgaacat tttatcatcc gttacatgta
     4621 ttttaagaat tttatgttct tccctcggca aatataatct ttcatcgggg cagttcataa
     4681 aaatacaatt tgtatcaggt tcatatttcc aaaaagtgca tgcaacgatt cgattaccga
     4741 taataccatt ttctcggtat ttatcaaaat tatacttgtc aacgagaata tcatctattg
     4801 gatatttgtt ataactgcca ccttcacgat actgaacaca catcagataa agcattttta
     4861 aagtatttat ggcaattttg ctattactac cttttccgcc gcttccatga tgtggagaag
     4921 aatctcgatt ttgcttagtt aataagtttg taaaaaagtt gtccaaagaa aagagatttt
     4981 cacagtaatc atctggttta aacaaatcct taatatgaca aagaattcct cctgtgtgtt
     5041 ctgatatatt ataactcaca aaacacgcac ttggcttttg cggagagcgc acatacattc
     5101 tggctcgaca atcaggtgtt gtgcaataaa atgttccatt accatgtaat ggcaggtcag
     5161 atgcatctac tctgtcattt tcagttccat tcggaactcg atatgcatat ttagccatat
     5221 taacctccat aagtacaacg tataaattaa attatgttaa gaaaggaatc caaaaaaata
     5281 tgaaacaagc aaaaaacgac aacaactcaa catattctat gactcataaa aatgcaattt
     5341 atgttgagct acacatatat cagtgatttt cccataagct cctctataat tcattcatgt
     5401 tgatgagctt gaatctaggg tctctgacca catagctttg cagtgcaggc tcaaaatctg
     5461 ttgccgaaaa cagataatag tcatcagatg taagccttgc ctgatccata gccatagcca
     5521 tattctcgca catatccatg gtcatcattg gcttgtccca gttgcagaag ccgatgatac
     5581 tccttctatc ctcgctctga gccacgatgt ctatgcggcc tgtctttcca atccatgtac
     5641 ccacacgctt tatggtaaac ggaagctgtc ccatctgatt taatataagc agatactctg
     5701 tgcagacctc cctgaaatat ctctcaagat acgcatcaag ctctttttct atatatgtat
     5761 cataaaactc ttccggtgaa agcagataca gattagacat attcggatag acaaacttaa
     5821 accagaaatt aacaaatgtg tcttttatct gatatacgcc cttctttgca ttttcccatc
     5881 cgccggtctc aaatgaaact actttttcca cgatattgaa gttggcaaga ttctttaaat
     5941 atacgctgat tttggcacgt gaaaatcctg tcgcatggaa aatatcattg agcttgttct
     6001 cgcctcttgc gattgctgca agtattgtgc tatatgcaga aagctctcga agctctgcgg
     6061 atataacagc atccgcctca ccatacagtg ctcccgatga tgtcaatacg agcctgcata
     6121 tattatcctt aaaggattta gctgcatccc acttatccag atatgccgga actccaccga
     6181 ttgctccgta aattctcacg atttccgcga ccggaagact ttcaaaatgt cttacaacct
     6241 caaggaaatt gagcgatcca agcttcattt cggctccctt aaactgctct gctgcctcct
     6301 gtgtagccca gactgtagat gatgtggcaa gtattataag tacaggtcct ggatacagct
     6361 tgtgcttttt aagctttgca actgcttcca taaatgatgt atccctcttt gccacaaact
     6421 gtgcctcatc gattatcacg acaagcttgg aagggtcacc tgatttgatt ctcgcgaagt
     6481 actcctcata tgtctgtctg ctcaggctca ccttgtactg acggcttatc tcctccccca
     6541 tcatctcaag ctgatgctct ggcgatgcct gtctgcatct gtagtaaaag aatttctttc
     6601 cgtcacaaaa ttcctttatg agctcctcct tttgacagcc tctccttccg tagagcacta
     6661 cgagctggtt acccgactct ttatagagct gctcaagttt ctctaaatct gtgtgttttg
     6721 ctttcataaa tattcctcca aaataatcaa ctggaaatat tatatcacaa tttaacacat
     6781 atacaaatag attttaatta ttatttccga gctcctattg tagctttcac tttatttctg
     6841 acaaacatat agcagatagc ctgcatgatg attgtcgcta tccatgaacc gggatatgat
     6901 ataaacagga aaaacagcga cctgtggctt ggaaacagac catatatcca gatgattctg
     6961 actccgactg ttcctattat agaaagtatc ataggtactg ttgagtatcc cattcctctt
     7021 agtgctccag gaaaaagatc catgattcca cacaggaaat atgggattgt tgtaatcgaa
     7081 ataatctcaa gtcctgattt aattacctcg ctattacttg tatatatgcc aagtatctgt
     7141 gttccaaata ggtatgaggc acttcccatc acaagtgcta ccgctgtcgt gagaaacaga
     7201 cagtctatca gtactctgtc cattctcttt tgttttccta ccgcatagtt ctggcttgta
     7261 aagctcatgc aggcctgtgt gattgagttg acagatacat acaaaaagcc aagtatattg
     7321 tttgccgccg tatatccagc catagctatt gagccaaagg aattgactga tgactgaagc
     7381 atggcatttg agatatttat gactgtgctc tgtatgccgg ccggtattcc cacctgaaat
     7441 atctgaagca tatattcctt atcaatcgca agcttattaa aacagagctt atagctggta
     7501 tctgctttgc tcagacagta taatacaagc acacaggata caaactgcga aacaattgta
     7561 ccgattgcaa cacctgcaac acccagtttg cacactatta caaaaaacag attaagcatt
     7621 gcattgatac agcctgaaat aatcaaaaag ataagaggtc tctttgtatc gccgacagca
     7681 cgcaaaattg ccgcgccata gttataaagc ataaaaaacg gcataccgca aaaataaatc
     7741 ctcatataaa tagtagaaag acctatgaca tcatccggtg tacccatgat ttccagcgca
     7801 ccccttgcaa aaataacacc gaccacaccc atcacaatac cacttatgag tgcaatcata
     7861 atagcggtgt gcaccgtctt agacatctca tcatccttgc ctgccgcata aaatcgggca
     7921 gcgagcacat tcgcaccaag cgatataccc ataaacaaat tgacaaacac attgataagt
     7981 gccgtagtcg agccaacagc cgcaagtgat ttgctgccac taaactgtcc cacgacaata
     8041 atatccaccg cattgaatgc cagctgtaaa atacccgaaa tcataagcgg caacgcaaat
     8101 gaaataagct tatccattat tgaaccgttc accatatcaa tttcatactt attattcttc
     8161 acttatctct acctcttaaa aaatattctc ctcagcctat ctattttacc acgataaaat
     8221 ataaattcaa gcgtaactat ttaaaacaac tgagaataaa aatcgagtag gaggcggtgg
     8281 c
//
LOCUS       DPUF01000194            7572 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_36878, whole
            genome shotgun sequence.
ACCESSION   DPUF01000194 DPUF01000000
VERSION     DPUF01000194.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_36878"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="DHV81_06890"
     CDS             complement(<1..737)
                     /locus_tag="DHV81_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCI94186.1"
                     /translation="MQSKFKRILFGGDYNPNQWPKEVWEQDMAYFKDAHINSATINVF
                     SWAKIQPSENEYNFTELDEIVDMLSNENYDIVMATSTAALPAWMVKKYPETMSTDYEG
                     RQHKFGARHNFCPNSLVYQKYAKALATELAKRYSCNKNISVWHINNEYGGYCYCDNCQ
                     KQFRVWLKDKYKTLDAVNDAWNTEFWGHTFYDWDEIVVPNELSEEAWGGMTSFAGIST
                     DYRRFYSDSMLNCYKLERDAVKAIIPDA"
     gene            902..1768
                     /locus_tag="DHV81_06895"
     CDS             902..1768
                     /locus_tag="DHV81_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI94187.1"
                     /translation="MPIYFGSNFAREPFQFDSVGNDWEQESVTRPEGYPLYHYLQTKE
                     GVGEVTVYSRNQAVSDNILSNSHTIELQKGQGILIAPNVPHSYHNKNISSGNSLPWKT
                     EFATFTGTMAAAFREMFDEKEYRLIDENKGMEVSEAINRAVEDFKERSSDTQMLSLDC
                     YGIMLLLSDQSNHTHDNDDQSYVRFIKPVIDAIEESYMDDISAKELADGVFVSQQYLS
                     RVFTEFMNCSVYEYLTNYRINKAKELLLINSRRKVQDIASDVGYSDASHFIVMFRKLT
                     GMTPAQFRKLHI"
     gene            complement(1725..2446)
                     /locus_tag="DHV81_06900"
                     /pseudo
     CDS             complement(1725..2446)
                     /locus_tag="DHV81_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743265.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
     assembly_gap    1980..2048
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2506..3906)
                     /locus_tag="DHV81_06905"
     CDS             complement(2506..3906)
                     /locus_tag="DHV81_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94188.1"
                     /translation="MEIIMFIIFIVTNLFIILCMQFAYTHAYKYENGMYLNVHIPSSH
                     KEDAEVTEIVTTGKRKMKHFQIANVIISIAICFIVFINIAVFVLVYIVWMFAYIFGII
                     HIPNNSHRKMYALKKQNGWIIEAQRKKVYIDTRVSAEASATTVSYKWHALFLITELAA
                     YIPYFMLGDTHYNILIISLFLCSVLISTLSLVFHAFINKSERHVYSMDSKLNLIVNNT
                     MKKYKSIAMLLLSGLNAVAWIYVALYTGITGILPASSYYVYIFIQLIAVLGFIVTIYM
                     GLNRKKELLSANTSPIDVDDDEYWKTGYYYNPADKHILIENRMQSGNYTFNYAKKGAW
                     IFTGITCAIIAGCIILVFVCMLPLINIQEKITLTNNNLTISAGGYTSEIDVNDITELK
                     LLDELPDDSFLRTNGASTNSYDIGRYEGRTLGKCSLYVFDGYSPILMIKSDDTLVFVN
                     SKEDGEIEKLYVELSQ"
     gene            complement(3922..4305)
                     /locus_tag="DHV81_06910"
     CDS             complement(3922..4305)
                     /locus_tag="DHV81_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI94189.1"
                     /translation="MIIELDMSSSTPIYVQLRNQIVKGIGKGELKTGEKLPTVRQLAS
                     DAGVNTMTVNKAYQILKNEGFIRTDRRLGAFVSESIADDTDFTEKLESELELLSAEAC
                     LTGMSREEFLSMCDKMYSQMKPCTA"
     gene            4558..5802
                     /locus_tag="DHV81_06915"
     CDS             4558..5802
                     /locus_tag="DHV81_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCI94190.1"
                     /translation="MSRLIEQIKQKDACAFTHGGKFHADDVFSSALLLYINPEISITR
                     GNSVPDDFTGIVFDIGRGEFDHHQKDSRIRKNGVPYAAFGLLWEAVGADILGEELAVK
                     FDESFVQPLDNNDNTGEKNELATLIGNFNPSWDYEGGSDEAFFQAVSVAGMILENKFE
                     RYRGNERADKRVEEVLAKHDPASRILVLPEFIPCQKALSETDIAFVIFPSNRGGFCIQ
                     PQKREYSMNYKCSFPAEWLGLEGEELVNATGIPGAVFCHKGGFIMTVKEQDEAVKACE
                     KALSLHKDSSVIVWYGSKGDTAAKACDSQTDELLINVAKARGIKGVHICHVDPMPVPQ
                     LELTELDSETAYAEVLMEKPQWKAYVKEQVKCILKYRPEAVYVEGNAFETYPVIRALR
                     KKHIPVLTMIENKEKKIMVRIP"
     gene            5933..7387
                     /locus_tag="DHV81_06920"
     CDS             5933..7387
                     /locus_tag="DHV81_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCI94191.1"
                     /translation="MKTIIKYELVINKALRSALNYDTPDEQINEFIRFFGKHIGSDRI
                     YIFEDCDEKHVTKNTYEWCADGIVPEIHELQNVDMDIIGWWYQAFDNEKNIIITDIEQ
                     IKDEHRGSYNLLKAQNVKNLVVCPIRYKDEIKGFFGVDNPPESDTLGLTTFLDMIGTL
                     LISLLKLRNSFTKSNKEAKLSSYSSLSSIYISMALVNVQTRRYHIVKTLDEVTHFLGV
                     NPQKEGEYRIDEDFPGHINSVMNEFCTKAQRKETLEFVDISTVEDRLRGKNTIVHEFI
                     GKVSGWCRERFIPVDYDADGRLWHVLYCVENIDEEKRREDRLMYLAQIDLMTGIRNRG
                     SGENKITEYLVRKQCGLLCLLDCDKFKSINDTYGHAVGDKVIIAIADTLRKSCRDDDV
                     VLRLGGDEFAVFIPGMLDKERAEAFFDRLFSNIEHIDIAEMNGKHILLSLGACFYDGM
                     DRITFDQLYRRADTAMYDSKKKQGYSATIFDAKQ"
     gene            7424..>7572
                     /locus_tag="DHV81_06925"
     CDS             7424..>7572
                     /locus_tag="DHV81_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HCI94192.1"
                     /translation="MQTGKAISIPSILKVGNGTLNKIGEYLKSEELTSVVIFMGNGLI
                     DMFGDT"
BASE COUNT         2195 a         1397 c         1772 g         2139 t
ORIGIN      
        1 gcatcgggaa tgatagcttt tactgcatcg cgctccagct tatagcagtt cagcatgctg
       61 tcagagtaaa accgtctgta gtctgttgat attccggcaa agcttgtcat gccaccccag
      121 gcctcctcgc tgagctcgtt aggtacgact atctcatccc agtcatagaa ggtgtgaccc
      181 cagaactctg tgttccaggc atcatttacg gcatcaagag tcttgtactt atccttgagc
      241 catacacgaa actgcttctg acagttgtca cagtaacagt atccgccgta ctcgttattg
      301 atgtgccata cagaaatatt tttattgcag ctatagcgtt ttgcaagctc tgtagcaaga
      361 gcctttgcgt acttctgata taccagggag ttcggacaga aattgtgtct ggctccaaat
      421 ttatgctggc gtccctcgta gtcggtgctc atcgtctcag gatatttttt gaccatccat
      481 gcaggcaatg ctgctgtgga tgttgccatg acgatgtcgt agttttcatt tgagagcata
      541 tcaactattt cgtcgagctc tgtgaaatta tactcattct cagacggctg tattttagcc
      601 catgaaaaaa cgttgattgt ggcactgttg atatgggcat ccttaaagta tgccatgtcc
      661 tgctcccaga cctccttagg ccactgattt ggattgtaat ctcctccgaa aaggattctt
      721 ttgaattttg actgcatttt tttcctccgt tatggtatta accccaatat tatgatattc
      781 ttaaactata aatgtgtttc cttatgcact aatcatataa tagatttacg gaaaatagta
      841 taacatcata aaccaaatta tataacattc tgaaactaaa ccgtttttga aaggagaaat
      901 catgcctatt tattttggaa gcaatttcgc aagagaaccg tttcaattcg actctgtcgg
      961 taatgactgg gagcaggaaa gcgtgacgcg cccggagggg tatccgctgt accattattt
     1021 acagacgaag gagggtgtgg gtgaggtgac ggtatacagc agaaatcagg ctgtttcaga
     1081 caatattttg tcaaacagtc atacgattga gctacaaaaa gggcagggga ttctcattgc
     1141 tccaaacgtg ccacactcct atcacaataa aaacatctca tccggcaata gcctgccctg
     1201 gaaaacagag tttgccactt ttacagggac aatggcggca gccttcaggg aaatgtttga
     1261 tgaaaaggaa taccggctga ttgatgagaa taaggggatg gaggtgtcgg aagcgattaa
     1321 cagggctgta gaagacttta aggaacgctc cagtgatacg caaatgctct cactcgactg
     1381 ttatggcatc atgcttttgc tatcagacca gtctaatcac acacacgata atgatgatca
     1441 aagctatgta cgttttataa agcctgttat agatgctata gaggaaagct acatggacga
     1501 tatcagtgcc aaggagcttg ctgatggtgt atttgtatcg cagcaatatc tatcgcgtgt
     1561 ttttacggag tttatgaact gctcggtgta tgagtacctg accaactacc gcatcaacaa
     1621 agccaaggag ttactgctta taaacagcag gcgtaaggtg caggatatag catctgatgt
     1681 ggggtactca gatgccagcc attttatcgt catgttcagg aaacttacag ggatgacacc
     1741 ggcgcagttt cggaagctgc atatataaca tcatctacaa tccgtattcc gtatttatca
     1801 aggtcaacct tgccgtctat gacctcaatg ccatctgctc ttagacggtt agcctgttca
     1861 tcggctccgc cgaaggcaaa tgcccctgac agctcacctt ttgcatttac caccctatag
     1921 catggaattt catcaggatt gggattcttg tgcagggcat tgccgacagc ccggcacatn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnac ctgtgcatat gtggcaacac agccctttgg aattcttttt acagcctcat
     2101 atatgagccg ggtcggatta taggtgacaa ggtcatagct gtctgcaatc atgtttttta
     2161 tagcatcatc cggtatgctg ccattgagaa tgatggtatt ccagttgtcc ttgttctggt
     2221 gccagccggg gatgaccgat gggaaggcat ccctccagaa gtctcgccag gcaggagata
     2281 ccttgacatt taagttgata aaaccattgc gctcgtaggt ccacaggaaa gcctttttgc
     2341 tgtcctttac gcggatgagc tgccagtttg ggtcgtggaa tggggcttct tgatatgtat
     2401 ttggaaaaga taaaccgtag tttaatgctt cttgtcgtgt tttcatagat tttgcctgct
     2461 ttctttttag gtatacgatt acagtataac ataaaaagaa caaatttatt gacttaattc
     2521 aacatataat ttctctatct ccccatcttc cttagaattt acaaacacca gtgtatcatc
     2581 cgattttatc atgagaatag gagagtatcc gtcgaataca tacaggctgc attttccaag
     2641 ggtgcgtcct tcatacctgc cgatatcata gctgtttgta gaggctccgt ttgtacgcag
     2701 gaaactgtca tccggcagct cgtccagaag cttaagctct gtgatgtcat ttacatcaat
     2761 ctcgcttgta tagccaccgg cagaaatagt aaggttattg tttgtcagag tgattttttc
     2821 ctgtatatta atcaaaggaa gcatgcagac aaacaccagg atgatacagc cggctattat
     2881 tgcacaggtg attcctgtga aaatccacgc gcctttttta gcataattaa acgtgtagtt
     2941 gccggactgc atgcggtttt caatgagaat atgtttgtcg gcgggattat agtagtagcc
     3001 ggttttccag tattcatcat cgtccacatc tattggtgag gtgttggcag acagcagttc
     3061 cttctttctg tttaagccca tataaattgt aacaataaat cccaaaacgg ctattaactg
     3121 aatgaaaata tacacataat aggatgatgc cggaagtatt ccggtgatac ctgtgtataa
     3181 ggctacataa atccatgcga cagcgtttag tccgcttagg agaagcattg cgatgctttt
     3241 atattttttc atggtattgt tgacaataag gttgagcttt gaatccatgc tgtatacgtg
     3301 gcgctcgctt ttgtttataa aagcatgaaa taccagcgaa agtgtggata ttaaaacaga
     3361 gcacaaaaac agcgaaataa taaggatatt gtaatgggtg tcaccaagca taaagtatgg
     3421 tatatatgct gcaagctcag taattaaaaa gagtgcgtgc catttatagc tgactgtcgt
     3481 ggcactggct tctgctgaaa cgcgggtgtc gatataaacc tttttgcgtt gggcttctat
     3541 tatccatccg ttttgttttt tcagagcata cattttccgg tgggagttgt ttggaatatg
     3601 aatgattcca aatatatatg caaacatcca gacaatatat accagaacaa aaacggcaat
     3661 gttaataaaa acaataaagc atatcgcaat ggagataatc acattggcaa tctgaaagtg
     3721 tttcattttg cgttttccgg tggtgacgat ttcggtgact tcggcatctt ctttgtgaga
     3781 tgatggaata tgtacattga gatacatgcc attttcatat ttataggcgt gtgtgtacgc
     3841 aaattgcata catagtatga taaaaaggtt tgtaactata aagataataa acatgataat
     3901 ttccatagtc agcctcctct gctaagcggt gcatggtttc atttgtgaat acattttatc
     3961 gcacattgat aaaaattcct cgcggctcat tccggtgaga caggcttcag cacttaaaag
     4021 ctcaagctct gattccagct tttcagtgaa atcggtatca tccgcgatgg attctgatac
     4081 aaaagcaccg agccgtctgt ctgtacggat aaatccctca tttttcaaaa tctggtaggc
     4141 tttattgacc gtcattgtgt ttacaccggc atcgcttgca agctgacgta cggtcggaag
     4201 cttttcgccg gtctttagct cgcctttgcc tattcccttt acaatctggt ttctaagctg
     4261 tacgtatatc ggggtgctgc ttgacatgtc gagttctatt atcatgtgaa cctcctgtat
     4321 cttaatgttt atatgtcata gcctgtttga aatgcatcaa tatttatgag caggctgtaa
     4381 tctgacaaaa cttgtaagtg atataaatgt attatctgta ttatatatag taatacagac
     4441 agcactttat gtcaactgat gttttagcat caattatatt gacattttga gtattacaat
     4501 tatgattaaa gagttataag gtatcgcaac gtaaaagaat acatatgagg ttaaaaaatg
     4561 agcagattaa tagagcagat aaaacaaaag gatgcctgtg cctttacaca tggcggcaaa
     4621 tttcatgcag atgatgtgtt ctcatcagcc ctgcttttgt atataaatcc tgagatcagc
     4681 ataacaaggg gcaacagtgt gcctgatgat tttacaggaa tagtttttga tatagggcgt
     4741 ggtgagtttg atcatcacca gaaggacagc agaatcagga aaaacggtgt gccgtatgcg
     4801 gcatttggac tgttgtggga ggctgttgga gcggatattc taggtgagga gcttgcggtg
     4861 aaatttgatg agtcatttgt ccagccactc gacaacaatg acaacacagg tgagaaaaac
     4921 gagcttgcaa cacttatagg caatttcaat ccgtcatggg attatgaggg tggcagcgat
     4981 gaggctttct ttcaggcggt aagcgtagca ggcatgatac tggaaaacaa atttgagaga
     5041 taccgtggca atgagagagc agataagaga gtggaggagg tgcttgcaaa gcacgatccg
     5101 gcaagcagga tactggtgct tcctgagttt ataccgtgtc agaaggcgct gagtgagacg
     5161 gatattgcgt ttgttatttt tccgtcaaac agaggcggct tttgtataca gccgcagaaa
     5221 agagagtatt cgatgaatta caagtgttct tttccggcag agtggcttgg gcttgaaggt
     5281 gaggagctgg taaatgctac aggaattccc ggtgcggtat tctgtcataa gggaggcttt
     5341 atcatgactg taaaggagca ggatgaagct gtaaaggcat gcgaaaaggc gctttctctt
     5401 cataaagaca gctctgtgat agtatggtat ggcagtaaag gagatactgc agcgaaggca
     5461 tgtgacagtc agacagacga gctgcttata aatgttgcaa aagcacgagg cataaagggc
     5521 gtgcatatct gtcatgtgga tccaatgcct gttccgcagc ttgaattaac agagcttgac
     5581 agtgagacag catatgcaga ggtgctgatg gagaagccac agtggaaggc atatgtaaag
     5641 gagcaggtaa agtgtatttt gaagtacaga ccggaggcag tgtatgtgga aggaaacgca
     5701 tttgagacgt atcctgttat acgtgcactc agaaaaaagc acattcctgt gcttacgatg
     5761 attgaaaata aagagaaaaa aataatggta agaataccat gatttatgtg catttttgaa
     5821 aaaatttggt ataatgttgt taagcgcgga catcgtgcag ttttgcgaat gtgtgtggtg
     5881 gttttgaata atacgggtaa tatacaagta acaatacgag gatgcagcag gtatgaagac
     5941 aatcataaaa tacgagcttg ttataaataa ggcactcaga tctgcactca actatgatac
     6001 accggatgag cagattaatg agtttatcag atttttcgga aaacacatcg gttctgaccg
     6061 catatatata tttgaggatt gtgatgagaa gcatgttacc aaaaatacat atgagtggtg
     6121 tgcggatgga atagtgccgg agatacatga attgcagaat gtcgatatgg acataatagg
     6181 ctggtggtat caggcctttg acaatgaaaa aaatatcatt attacagata tagagcagat
     6241 aaaggatgag cacaggggct cttacaatct gttaaaggca caaaatgtaa aaaacctggt
     6301 ggtatgtcca atccgttaca aggatgagat aaagggcttt ttcggtgtgg ataatccacc
     6361 ggaaagtgat actctcggac tgaccacatt cctggatatg ataggaaccc tgctcatatc
     6421 attgcttaag ctgcgcaatt ccttcacaaa gtcgaataag gaagcgaagc ttagcagcta
     6481 ttcatcgctt tcaagcatct atatatccat ggcactggta aatgtgcaga cacgcagata
     6541 ccatatagta aagactttgg atgaggttac acactttttg ggcgtgaatc cacagaaaga
     6601 gggagagtat aggatagatg aggatttccc agggcatata aacagtgtga tgaatgagtt
     6661 ctgtactaag gcacagcgca aagagacact tgaatttgtg gatatcagta ctgtagagga
     6721 cagacttcgt ggaaaaaata ccatagtgca cgagtttatc ggaaaggtat caggctggtg
     6781 ccgtgaaagg tttattcctg tagactatga tgcagatggc aggctgtggc atgtgcttta
     6841 ctgcgtggag aatattgacg aggaaaagcg acgtgaagac aggcttatgt atctggcaca
     6901 gattgatttg atgacaggta tacgcaaccg cggaagtgga gagaataaga taacagagta
     6961 tctcgtccgc aaacagtgcg gtctgttatg ccttttagac tgtgacaagt ttaagtcaat
     7021 aaacgatacc tatggacatg cggtaggtga caaggtgata atagcaattg ctgatactct
     7081 gcgcaaatca tgtcgtgatg atgatgtcgt gcttcgcctg ggcggggatg agtttgcggt
     7141 atttataccg gggatgcttg ataaagagcg ggcagaagct ttttttgaca ggcttttttc
     7201 aaacatagag catattgata tagcagagat gaatggaaag catatactgc tcagtcttgg
     7261 cgcatgcttt tatgacggaa tggatcgtat cacgtttgac cagctctaca gaagggcaga
     7321 caccgccatg tatgacagca agaaaaaaca gggctacagt gcgacaattt ttgatgcaaa
     7381 gcaatgatag tccggcatat taaataaaaa gaggagaatg aaaatgcaga caggaaaagc
     7441 aatatccatt ccatcaatcc tcaaggtggg aaatggaaca ttaaacaaaa tcggagagta
     7501 tttaaagagt gaggagctga cgagcgtagt catttttatg ggaaacggtc tcatagatat
     7561 gtttggagat ac
//
LOCUS       DPUF01000195            9445 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1482, whole
            genome shotgun sequence.
ACCESSION   DPUF01000195 DPUF01000000
VERSION     DPUF01000195.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1482"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            468..904
                     /locus_tag="DHV81_06930"
                     /pseudo
     CDS             468..904
                     /locus_tag="DHV81_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369234.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            915..1253
                     /locus_tag="DHV81_06935"
     CDS             915..1253
                     /locus_tag="DHV81_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94193.1"
                     /translation="MKTRFQKICQSGCVCSLKYLELIFGNIKCNLIMKLLRLQNMKEK
                     YRDFHFGEFIEKPNQCGYFEQNGNWYTYVIDERNFCIFTGPFNGSAIIYAFSIEAGNG
                     RLWESEKYKF"
     gene            1500..3020
                     /locus_tag="DHV81_06940"
     CDS             1500..3020
                     /locus_tag="DHV81_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCI94194.1"
                     /translation="MQDISIIGIIVTVVVLVILLFMAIGYVKAPPDMAYIISGVKKKS
                     KVVIGKASIRIPFFERLDKLNLRLIPIDVKTSNAVPTADYININVDATVNVKISNNPE
                     KLRLAAENFLNKNTEYIAGVAREVLEGNVREIVGKMKLEEMVSDRQKFANLVKENAEP
                     DLAAMGLDIISFNVQNFVDGNEVIENLGIDNIVKIKKAAAIARAESERDIKVAQASAD
                     KESNDAAVAAQTEIAKKQNELAIKKSELQMEADTKKAMADAAYDIQKEEQRKTIEVAT
                     ANADIAKQEREIELKQKEVAVTEQSLEAEVKKKAEANKYAAQQQAEAQLYQRQKEAEA
                     RQFEAQRQAEAQKAEAEAMRYAKEQEAAGIRAVGEAEASAIQAKGIAEAEAMEKKAEA
                     YAKYNKAAVAEMMIKVLPDIAAKVAEPLGQIDKITIIGGGDGENGVGKVAGNVPVVMA
                     KVFESMKEATGIDLSEIINADSYDAKVNRNVNVTGLDGVNLVVNADTDKNGEKTEN"
     assembly_gap    3185..3253
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3254..4648
                     /locus_tag="DHV81_06945"
     CDS             <3254..4648
                     /locus_tag="DHV81_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25"
                     /protein_id="HCI94195.1"
                     /translation="AAVATFFVIKSAKNEAKGSSQMTETATEDSSDKEDPQHTGDNAG
                     SMSVTTDEGASADETQILSASTVAENSNLTFGIDVSKYQGTIKWDKVAAGGIDFAMIR
                     VGYRTQKTGEMCVDTNARYNMQEAQKNGIKVGVYFFSSAVNETEAIEEADWVADYISK
                     YQITYPVAFDCEGFTDSASRQYGMSSEARTKVAEAFLQEIYNKGYTPMFYAAMNELSA
                     NSQWDTKTLESRYKIWVSQYPDTAYPETPQSSYEGTHAMWQYTNKGKVSGIDKPVDLN
                     VAYFGFDETESAKNGDAADDATADPEANMKFSDVNETVTAKESVNLRDIPSQGNDSTI
                     KVTLNNGDTATRTGVSDSGWSRVEYNGQTYYAISNYLTTDMNYVVPGQDGSGSGDGLK
                     TKFKECNDTVTAKIEVNLRALPSVTNPDATVVATIKNGETVTRTGINEEFGWSRVEYN
                     GQTLYCVTSYLSVQ"
     gene            4821..6554
                     /locus_tag="DHV81_06950"
                     /pseudo
     CDS             4821..6554
                     /locus_tag="DHV81_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744048.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     assembly_gap    5732..5825
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6798..8231
                     /locus_tag="DHV81_06955"
     CDS             6798..8231
                     /locus_tag="DHV81_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HCI94196.1"
                     /translation="MKRHKICKIILAILAVFLCVAFYELIGICITYKKQPTVSDATIK
                     ETQNIMSVAGRENTERAVIIEKNSQALLERVRLIQNAKDEIILSTFAFKSDESGKLIL
                     GALHDAADRGVHVRILVDGMESWIDMEGNPYFYGLSSHENVEIKLYNKANPLKPWKTM
                     GRMHDKYLIADGKIYILGGRNTYNYFLGDFPGHKNFDRDVLVVCDEPQKDNSVNQLWN
                     YFETIWEQEDCRYFHNSKKLADRQSVKKAVLELQEGYQQYFEVNKEKICDTDYADETF
                     ETEKIILLSNPIHTQAKEPVVWYQLGELMKNAKERVKIHTPYIICNDMMYNTWEEIAQ
                     KVSDFSIMTNSVANNGNPFGAADYAKNRNRILETGIDIWEYEGGYSYHGKSILIDDDL
                     SVIGSFNMDMRSTYLDTELMLVIRSKEINKQLEDGMMEYESVSRQVLDDGTYYDPYHV
                     KPIELTEKHKRKVFLVQHLLGWARDLF"
     gene            8251..8922
                     /locus_tag="DHV81_06960"
     CDS             8251..8922
                     /locus_tag="DHV81_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI94197.1"
                     /translation="MFKILIVEDDKELSLLFQKVLEKSGYQVKSASDGAQALEVLDRE
                     YIDLIISDIMMPVMDGYELVSELRSAGYQIPVLMITAKSSFDDMRQGFLSGSDDYMVK
                     PVNVNEMVLRVGALLRRAQILNEHKIVIGSTEFDYDAMTVVTGDETLVLPKKEFLLLY
                     KLAASPGRTFTKQQLMDEVWGYETEADPHTIEVHIGRIRERFKENPDFEIVTMRGLGY
                     KVVKK"
     gene            8922..>9445
                     /locus_tag="DHV81_06965"
     CDS             8922..>9445
                     /locus_tag="DHV81_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI94198.1"
                     /translation="MEYKKKKELRIRSCLTGAIWLALAFSMLISALLFAFLNHFLDLP
                     GKIPGLGWLLIFNTLIAGLITSFINARLLEPITRLSKAMKAVSQGDFEQHLETNSRIA
                     EIGESYQSFNVMTKELRATEMLQMDFVSNVSHEFKTPINAIEGYTMLLQGDELSQEQE
                     GYVEKILFNTQRLSG"
BASE COUNT         3199 a         1403 c         2264 g         2416 t
ORIGIN      
        1 cctctgcatg tctattaatt aaccatcata gctgttgcga agcaattaag aatccatgat
       61 gttaattaat agacactagc gaaattttgc cgagctgggt atacgaacaa atattgcaaa
      121 atgaatatgc tgatgtaacg cactcctagc aacagaatat tgcgaaagct ttgaagcaaa
      181 agaaaaaaac aatcctcagg gcacaaacag ctctgaggga tttttttcgc aaaaaatgaa
      241 aaaagtagag aaatttttcc ggtgtgactg ctgtgtgacc accatagtgt atactgtaat
      301 gtagaaagaa ataacaaaga aaacgatatt aaatcgggaa taaaatgtga ggtgaagagt
      361 gattaatgag aaaactgaat ttttaccact gggaagtatc gtggttgtaa agggatatta
      421 attcctgttg acaatattaa attaaataaa tagggaaatt aatgtgtatg aataatatta
      481 caaaaaatag attgattcag gttggaaaga acaacgaaaa attaatactg atttgatccg
      541 aaagaaatat gatgaaattg gattagaata tccagaagtt gtagataaat tcttaaaaga
      601 atatggcatg ttaaatataa atcctagaga taagtgctat tttgatgttt cgtttaatgc
      661 aatcaaagca attggatgta atcttgatgg ttcatatttt aaagagtgtt tagctgaata
      721 tgatataaat gaaacagtat atccaattgg agaagcttgt agaaagaatt tattagtact
      781 aatgacatta acagaatcgt tttattgttt tacagatggg ctattattat ttttgggtgg
      841 ttcagttgat gaaatgcttg attgtatagt aggagaatgt agaaagccaa tagaaattga
      901 ataggtgata attgatgaag acaagattcc aaaagatatg tcaaagcgga tgtgtatgca
      961 gtttgaagta tctagaattg atatttggta atatcaaatg caatttgata atgaaattat
     1021 tgaggctgca gaacatgaag gaaaagtata gagatttcca ttttggagaa tttatcgaaa
     1081 agccaaatca gtgtggatat tttgaacaaa atggaaattg gtatacatac gtaatagatg
     1141 aaagaaattt ttgtatattt acaggaccat ttaatgggag cgcaataata tatgcgtttt
     1201 ctatagaagc tggaaatgga agattatggg aaagcgaaaa atataagttt tgataaaaat
     1261 acaggagaaa tacaagggat gatgattaca aaataaaatt gatgaataag taatgcctgc
     1321 tgattaagaa gatatctgta atttctaaat tttatgtttg catcgcttat accataatca
     1381 gaacaaatgc acagtggtat attatttttt taatatttag agtgacaaaa caatatattt
     1441 ataataaaat gatattgtta aattattaat aaaaagcata ctaaagagag gagaaagtta
     1501 tgcaggacat tagtatcatt ggaattattg ttacagttgt tgttcttgtt atcctgttgt
     1561 ttatggcaat cggatacgtg aaggcaccgc ctgatatggc gtatatcatt tcaggtgtga
     1621 agaaaaagtc aaaggtagtt atcggaaagg cgagcatacg tattccgttt tttgagcgtc
     1681 ttgataagct gaatctcaga ctgataccta tcgatgtaaa gacgagcaat gcagtaccga
     1741 cagcagatta tatcaatatc aacgtcgatg ctacggtaaa cgtaaagatc agtaataatc
     1801 ctgaaaagct acgccttgcg gctgagaatt tccttaacaa aaacacagag tatattgctg
     1861 gtgttgccag agaggttctc gagggtaatg tacgtgagat tgttggaaaa atgaagcttg
     1921 aagagatggt ctcagaccgt cagaagtttg ccaatctggt aaaagagaat gcagagcctg
     1981 atcttgcggc aatgggactt gatatcatca gctttaatgt gcagaatttt gtggatggca
     2041 acgaggtaat agagaatctt ggtatcgaca atatcgtaaa gattaaaaag gccgccgcca
     2101 tcgcaagggc agagagtgag agagacatca aggtagctca ggcttcggct gacaaagagt
     2161 caaatgatgc agcggttgca gcacagacag agattgccaa gaaacagaat gagctcgcta
     2221 tcaagaaatc agaacttcag atggaggctg atacaaagaa ggctatggcg gatgcggctt
     2281 acgatattca gaaggaggag cagcgtaaga ccattgaggt tgccacagca aacgctgata
     2341 ttgcaaagca ggagcgagag attgagttaa agcagaaaga ggttgcagta acagagcagt
     2401 ctcttgaggc agaggtcaag aaaaaggctg aagccaataa atacgcggca cagcagcaag
     2461 cagaggcaca gctctaccag cgtcagaagg aagctgaggc aagacagttc gaggcacagc
     2521 gtcaggcgga ggcacagaaa gcagaggcag aagccatgag atatgccaag gagcaggaag
     2581 ctgccggtat ccgtgcagtc ggagaggcag aagcatcagc tatccaggca aagggtattg
     2641 cagaggctga ggctatggag aaaaaggcag aggcatatgc caagtacaat aaggcagccg
     2701 ttgcagagat gatgattaag gtgctgcctg atatcgcagc caaggttgca gagccacttg
     2761 gacaaatcga caagattact attatcggtg gcggtgatgg cgaaaatggt gtaggcaagg
     2821 ttgccggaaa tgtaccggtt gtaatggcaa aggtatttga gagtatgaaa gaggcaacag
     2881 gtattgattt atctgagatt atcaacgctg attcatatga cgcaaaggta aatcgcaacg
     2941 taaatgtaac cggacttgat ggagtaaatc tcgttgtaaa tgcggatacg gacaaaaacg
     3001 gtgaaaagac tgaaaattaa atagtgttat attaatccgc tatacgatag gtgagtatag
     3061 cggattttta atattttctg aaaaaagaca aatatttgaa aattacagga ttatatgata
     3121 tattttgttg cataaaagca gattgcctgt acatggtgac atatataaat aatgaagagg
     3181 aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnngctgcag ttgccacatt ttttgtgatt aagtcagcaa aaaacgaggc
     3301 taaaggctcg tcacagatga cggaaacagc cacagaggat agttcagaca aggaagaccc
     3361 gcagcatacg ggcgacaatg ccggctcaat gagtgttaca actgatgagg gagcttctgc
     3421 ggatgagaca cagatactgt ctgcaagcac tgttgcggaa aatagtaatc tgaccttcgg
     3481 aattgatgta tctaaatatc agggcaccat aaagtgggac aaggttgccg caggcggaat
     3541 tgattttgcg atgatacgtg tgggctacag aacccagaaa acaggtgaga tgtgtgttga
     3601 tacaaatgcc agatacaaca tgcaggaggc acagaaaaat ggcataaagg ttggagtata
     3661 tttcttttca tcagcagtaa atgaaacaga agccattgag gaggcagact gggtagcaga
     3721 ctacatctca aaatatcaga ttacatatcc ggtggcattt gactgcgagg gcttcacaga
     3781 ctcagccagc agacagtatg gaatgtcatc agaggcacgc accaaagtag ctgaggcatt
     3841 tttgcaggaa atatataata aaggatatac acctatgttt tacgctgcaa tgaatgagtt
     3901 gtcggcaaac agccagtggg atactaaaac acttgagagc aggtacaaaa tctgggtgtc
     3961 acaatatccg gacacagctt acccggaaac accacagtca tcttatgagg gcacacatgc
     4021 catgtggcag tataccaata agggaaaggt gtcagggatt gataagccgg tagatttgaa
     4081 tgtggcatac tttggttttg atgaaacaga gtctgcaaaa aatggggatg cggctgacga
     4141 tgcaacagct gatccggaag caaatatgaa gttttcggat gtaaatgaga ccgtaactgc
     4201 caaggagagc gtcaatctga gggatattcc aagtcagggc aatgacagca caattaaggt
     4261 aacgctgaat aacggtgata cagcaacaag aacaggcgtc agtgacagtg gatggtcacg
     4321 tgtcgaatat aatggacaga catattatgc catttcaaac tacctcacaa cagatatgaa
     4381 ctatgtagtt cccgggcagg acggaagtgg cagcggagac ggactgaaga caaagttcaa
     4441 ggagtgtaat gatactgtca ctgcaaaaat agaggttaat ctgcgtgcac ttccaagtgt
     4501 cacaaatccg gatgctacag ttgttgcaac gataaaaaat ggggaaaccg tcaccagaac
     4561 cggaatcaat gaggagtttg gctggtcacg cgtggaatat aatggacaga cactttattg
     4621 tgtgacaagc tatctgagtg tgcagtaata taatgaacgc agtgctttgg agaataaagt
     4681 caaaaaaata aaatgttatt aatctaaaat catgcataaa gtagaaatag tgtaaaaatg
     4741 tcatggatat aatgatagaa ttgtttgaaa aagtgcgtta aaatacataa taacgaaata
     4801 cgtaagtaaa ggggcatttt atggaatatt cagcaatttt tcatgatatg gataaaagat
     4861 tctgctatgc gatagataag gatttgtttg tgatacgcgt gcaggtaaaa aaggatgata
     4921 tgaaggaggt aatccttcac tatgaggaca agtatatacc aatcgagcgc aaggatacgc
     4981 gaatgacact tcctatgaag aaggtagcca cctcgcagtt tcacgattat tatgaggcgc
     5041 agcttcagat gcatctgata tgtctgagat acttttttga atttacggac atgcagggtg
     5101 aaaaggtata ttacggcaat tatgagttcg ataaggagtg catcacaaac agggacagaa
     5161 tgtttgactg cccgcagaat ctcagagagg aagagatgtt tgaggtgcca cagtgggcag
     5221 ccaacaaggt agtgtatcag atttttccat cgcgctttgc cacaacacag ccggtggaca
     5281 aagagctgtg gtataaagcg ccgattacgc ctatggatga tttgcatggc aatctcaggg
     5341 gaatcataga gcatctggat tatattaaag atctgggaat cgatgtggtg tatctgacac
     5401 caatctttaa atcaaattca tgccataagt atgataccat tgattattat caggttgacc
     5461 catcatttgg cacaacagag gacttaaagg agcttgtaca aaaatcccac gagcgcggca
     5521 tgaaggtagt gctggatgcg gtatacaacc actcaggaag agaatttttc gcatttcagg
     5581 atatacttga aaagggcgaa aagtcaaaat atctcgactg gtattttatc gacgagctgc
     5641 cgcctagggg tgaatgggga gaaattccaa acttcaaatg cttcggctat tacggtggaa
     5701 tgccaaagct taatctgaaa aatcctgagg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnngaaat cagtcatttc ttctggaaga atttcagaaa ggcaatcaag gcagtaaaga
     5881 aggatatgct gataattggt gagatatggc actacgcagg agacttccta gagggagatg
     5941 agtgggatac tgtgatgaat tatccattct acctgaatct gatagacctg cttgcagacg
     6001 aaaagattaa tgtcagccag tttgtacaga atctgggata tctgaagggg cgcttaaaca
     6061 aaaagtgcta tccgctgatg tggaacctga ttgacagcca cgatacagca agatttttac
     6121 atctgtgcca tgataataag aaaaagcagc atctcgcagc tgcctttcag ctgctaatgc
     6181 cgggaatgcc tatggtctac tatggtgatg agtatgcaat gccgggagca aatgatccgg
     6241 actgcagacg aggcatgtac tgggatgagg aataccagga caaggagatg tataattggt
     6301 ataagaagct catgcagatt agaaaagcac atgcatgcat cgtagagggc gaaatgattg
     6361 agacaatcac aaatgacgat gatgatacga ttgttatgat cagaaaaaac ggcgaagaga
     6421 caatcgcaat gcttttcaat tgtggcagca gtgtaaagga atttaatgag tatgccgaga
     6481 aatataatct gctgacagat agcgcatttg acggaaaagt agacgggctt gacgctgccg
     6541 taattgtgct ttaaaaatta tatggattat ctataagaag tcatggagct ttgcacatca
     6601 ggtaaacgga aagacggtag aagcgacgaa aaataggtaa atcgcagtaa aataatttta
     6661 tatgagtttt tatggacagg aaactgcagg cagctgcttt ttcctgtctt tttgcataaa
     6721 atcaattctg ttgaggtaaa attgagattg acattgtatt gtatgagtat aaaatacaag
     6781 gaatcaggtg atgtacaatg aaaaggcata aaatatgtaa aattattttg gctatactgg
     6841 cagttttttt atgtgtggct ttttatgagt taatcggaat ctgcatcaca tataaaaaac
     6901 agccgacggt gtctgatgct actataaagg aaacacaaaa tattatgagt gtggcgggga
     6961 gagagaatac ggaaagagca gtaatcattg aaaaaaattc gcaggctctt ttagaaagag
     7021 tgagattaat ccaaaatgca aaggatgaaa taatcctttc tacatttgca tttaaatcag
     7081 acgaaagcgg aaaattgatt ttaggagcac tgcatgatgc ggcagacaga ggagtacatg
     7141 tccgcatatt ggtagatgga atggagagct ggattgatat ggagggtaat ccatattttt
     7201 atggattatc ttcgcatgag aatgttgaga ttaagctgta caataaagca aatccgctta
     7261 agccatggaa gacaatggga agaatgcatg ataaatatct gattgcagat ggtaaaatat
     7321 atattcttgg cggaagaaac acatataatt attttttggg tgattttccg gggcataaaa
     7381 attttgaccg ggatgtgtta gttgtctgcg atgagccaca gaaagataat tcggtcaatc
     7441 agctttggaa ttattttgaa acaatatggg aacaggagga ctgtcggtat tttcataaca
     7501 gtaaaaaatt agctgacaga caatctgtaa agaaagcagt tttagagctg caggaagggt
     7561 atcagcaata ttttgaagtt aataaggaaa aaatttgtga tacagattat gcggatgaaa
     7621 cctttgaaac agaaaagatt atattgttat caaacccaat ccacacccaa gctaaggaac
     7681 cggtggtatg gtatcagctg ggagaattaa tgaaaaatgc aaaggaacgc gtaaaaattc
     7741 atacaccata tattatttgc aatgatatga tgtataatac atgggaggaa attgcacaga
     7801 aggtttcgga cttttctatt atgacaaatt ctgttgcaaa taacggaaat ccatttggag
     7861 ctgctgatta tgccaaaaac agaaacagaa ttttagaaac aggaattgat atctgggagt
     7921 atgaaggcgg ttattcgtat catggaaaaa gtattctgat tgacgatgat ctgtcagtaa
     7981 taggttcatt caatatggac atgagaagta cttatctgga tacagagctg atgcttgtaa
     8041 tacgtagtaa agagattaat aaacagctgg aggatggcat gatggagtat gagagcgtgt
     8101 ccagacaggt attagacgac ggaacatatt atgatccgta tcatgtaaag ccgattgaat
     8161 taacagaaaa acataaaaga aaggtgtttc tggtacagca tctgcttgga tgggcaaggg
     8221 atttgttttg attaaaggag aacataaatc gtgtttaaga tattaattgt agaagatgat
     8281 aaagaattaa gtctgttatt ccaaaaggtg cttgaaaaga gcggatatca ggtcaaaagt
     8341 gcgtcggatg gagcacaggc actggaggta ttggacagag agtatattga tttaatcatt
     8401 tccgatataa tgatgccggt tatggatgga tatgaacttg tgtcggagct tcgttcggcg
     8461 ggatatcaga taccggtgct tatgattact gcaaaaagca gcttcgatga tatgcgtcag
     8521 ggctttcttt cgggaagtga cgactatatg gtaaaacccg taaatgtcaa tgaaatggtt
     8581 ttgagggtag gagcactgct tcgtcgtgca cagatactga acgaacacaa aattgtgatt
     8641 ggctcaaccg agtttgatta tgatgcaatg acagttgtga ccggtgatga aacgcttgtc
     8701 ctgccaaaaa aagaatttct gcttttatac aagctggcag cttcaccggg caggacattt
     8761 acgaagcagc agctaatgga tgaggtatgg ggatatgaga cggaggcaga cccacatacg
     8821 atagaggtac atataggaag aatcagggag cgttttaagg aaaatccgga tttcgaaatt
     8881 gtaacaatgc gtggtctggg atacaaggtg gtgaaaaaat aatggagtat aaaaagaaaa
     8941 aagaattgcg tatccgctca tgtctgaccg gtgcaatatg gctggcactt gcattttcta
     9001 tgctgatatc agccctgttg tttgcgtttt tgaatcattt tttggatttg cccgggaaaa
     9061 taccgggact tggctggctt ttgattttta atacattgat agcaggactt attacatcat
     9121 ttatcaatgc aaggctgctg gagccgatta ccaggctcag taaagcaatg aaagcagttt
     9181 cccagggaga ttttgaacag catttggaaa caaacagtcg catagccgag attggagaat
     9241 cctatcagag ctttaatgtc atgacaaaag agcttcgtgc aacagagatg ttgcaaatgg
     9301 attttgtatc caatgtttcc catgaattca agacaccaat caatgcaatt gaaggctata
     9361 cgatgctgct ccagggagat gagctgtcac aggagcagga ggggtatgta gaaaaaatcc
     9421 tgtttaatac tcaaaggctt tcagg
//
LOCUS       DPUF01000196            4300 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_24763, whole
            genome shotgun sequence.
ACCESSION   DPUF01000196 DPUF01000000
VERSION     DPUF01000196.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_24763"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            1..756
                     /locus_tag="DHV81_06970"
                     /pseudo
     CDS             1..756
                     /locus_tag="DHV81_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568736.1"
                     /note="too many ambiguous residues; incomplete; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
     assembly_gap    432..528
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            774..2129
                     /locus_tag="DHV81_06975"
     CDS             774..2129
                     /locus_tag="DHV81_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCI94199.1"
                     /translation="MKRESFKSRLGFILVSAGCAIGIGNVWRFPYVTGQNGGGIFVLF
                     YLIFLVCMGLPVLTMELAVGRASRKSAVMSYKALEKEGSKWHIHGWIAMFGCYMLMMY
                     YTTVSGWMVAYFFKFLKGDFTSGMNADDAAAAFGSLLADPKQMAFWMIITVVLGFLVC
                     SRGLQNGLEKISKFMMSALLLLIIVLAVHSITLSGALEGVKFYLVPNLDAVSEIGIKN
                     VITAAMNQAFFTLSLGVAAMEIFGSYMGKEHTLTGEGAKICALDTFVAIMSGLIIFPA
                     CFSYGVEVGAGPSLIFITLPNVFVNMQGGRIWGCLFFMFMTFASFSTVIAVFENIMSF
                     AIDMFGWSRNKAALVNGVIILIASMPCVLGYNVWSNLHLIGGRDVLDSEDFMVSNLLL
                     PIGSLVYLLFCVTKWGWGFDKYIDEANTGAGLKISRKLKPYFQFILPLLILFIFIQGL
                     I"
     gene            2169..3074
                     /locus_tag="DHV81_06980"
     CDS             2169..3074
                     /locus_tag="DHV81_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94200.1"
                     /translation="MLDQNTFIETVQSVAEIIRTSAEPIGRDEIMSYFKDMELNKAQE
                     DMVFEFLITPHEQENESAGDYEDESNEDELHGDEASENAALNNIIYSEGDSMDAIAGG
                     KNNKDNQDNEAEPDIDEDSLIPDSPMFRMYLEELEEMPDYTKAEQDDMYKKLLAGDES
                     VIHTISNFWLKSVLAMAKKLAVTSEGFEDVVQEGNMGLFMCLTELCGCGDEIDVEKEL
                     LDAIEESMKACIREQTGEAESENTVVGKVSLVNRAVEQFKSNKGCEPSIGELSGYTGV
                     DEEELEKLLELIKKADVEKNKQDSR"
     gene            3143..3994
                     /locus_tag="DHV81_06985"
     CDS             3143..3994
                     /locus_tag="DHV81_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94201.1"
                     /translation="MARFTNQAQLRYGNNVANSNIAVGEILEVLSATKTAVKNTYNQN
                     DTITYVVSIVNSGNTAINGLTLSDNLGAYTFNTNTLVPLTYVNNTVKYYTNGTLQAAP
                     AVTQGPPLSITGINVPAGGNATVIYEAALNEYAPLGTEASVTNTATVSGTGITPVTAA
                     ETVNAEAAPNLAITKSVSPVPVTENGTLTYTFRIQNYGSVAATNTTGVVITDTFAPVL
                     SNLTAALNGTAWTAATDYTYNEATGTFSSTAGAITVPAATYTQDSTTGAWVVTPGEST
                     LVITGTV"
     gene            4125..>4300
                     /locus_tag="DHV81_06990"
     CDS             4125..>4300
                     /locus_tag="DHV81_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI94202.1"
                     /translation="MINRGIRQMNNAQLVIDEVKKAVKGKDDCIIKAFAAFLAGGHIL
                     LEDVPGVGKTTLAVA"
BASE COUNT         1224 a          743 c         1092 g         1144 t
ORIGIN      
        1 atcatggcgg tgtgctattt tgtcataacc atttttatca taattaaaaa tataggcatg
       61 atgccatcgg ttttttcaag gatatttgag gaagccttcg gtatcaggca ggtggctgcg
      121 ggaggctttg gagcggtgct catgaatggt gtaaagagag gtcttttttc aaatgaggca
      181 ggctcaggct cagctccatg cgctgcagcg gcggcagagt gcgatactcc tgtaaaggca
      241 ggactgacgc aggcgctcgg tgtatttata gatacgatag tgatttgcag ctgtacggca
      301 atgatcatgc tcactgcacc ggagaatata gtggcaggga aagagggcat ggatttatta
      361 cagtcagcta tgaggtatca tcttggagat tttggagtta tttttatagc agtcacattg
      421 tttttattca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat gtacaaggtg
      541 ctggctcttg ttatgctgtt tgtaggcggt atcgcggcat acacatttgt ctgggatctc
      601 ggagatgtag gaatcggcct tatgactata ttcaatacag gtattctcta tctgatgggt
      661 ggacaggcaa taaaggagct gcgagtcttt gaggagagta ataaagcaca aagcaatagt
      721 caatgctcac agcagggtga taacgtaacg caataacaat aggagatata tatatgaaaa
      781 gagaatcatt taaatcacgt ctgggattta tactggtaag tgcaggatgc gctataggaa
      841 taggcaatgt atggcgtttt ccgtatgtga cagggcaaaa cggtggaggc atttttgtgc
      901 tgttttatct tatatttttg gtatgcatgg ggcttccggt gcttactatg gagcttgctg
      961 taggccgagc cagcagaaaa agtgctgtga tgagctacaa ggcacttgag aaggagggca
     1021 gtaaatggca tattcacgga tggatagcca tgtttggctg ctacatgctc atgatgtatt
     1081 acacaacagt ctcaggctgg atggttgcgt atttctttaa attcctaaag ggagatttta
     1141 catcaggcat gaatgcagat gatgcggcag ctgcatttgg aagcctcctt gcagatccaa
     1201 agcagatggc tttttggatg ataattacgg tagtacttgg ctttttggtg tgcagccgtg
     1261 gtctgcagaa tggacttgag aaaatcagta aatttatgat gagtgctctg cttttactta
     1321 ttatagtcct tgcagtgcac agtatcacgc tttcaggggc attggaagga gtgaaatttt
     1381 atcttgttcc aaatctggat gcggtttctg agattggaat aaagaatgtc ataacagcag
     1441 ccatgaatca ggcgtttttt acgctgagtc tgggtgtagc tgcgatggag atattcggca
     1501 gctatatggg aaaggaacat actcttacag gtgagggtgc caaaatatgt gctcttgata
     1561 catttgtggc tatcatgtca ggtcttatca ttttcccggc ttgctttagc tatggggtgg
     1621 aggtaggcgc aggaccaagc cttatcttta tcactttgcc aaatgtattt gtaaatatgc
     1681 agggaggccg tatatggggt tgccttttct tcatgtttat gacctttgca agcttttcaa
     1741 ccgttatcgc agtatttgag aatatcatgt catttgccat tgatatgttt ggctggtcaa
     1801 gaaacaaggc tgcgctcgta aatggagtga taattctcat tgcaagtatg ccgtgtgtgc
     1861 ttggatacaa tgtgtggagc aatctgcacc tcataggagg cagggatgtg cttgacagcg
     1921 aggactttat ggtaagcaat ctgcttcttc cgattggctc ccttgtctat ctgctttttt
     1981 gcgtgacaaa gtggggctgg ggctttgata agtatataga tgaggcaaat acaggagcag
     2041 gacttaagat ttcaaggaag cttaagccgt attttcagtt catactgccg cttttaattt
     2101 tgtttatttt tatccaggga ttgatatagt tatgtattat ttggaaatca gacaggagag
     2161 tgtaaaacat gttagatcag aatactttta tagagacagt acagagcgtg gccgagatta
     2221 tccgtacttc agcggagccg ataggcagag atgaaatcat gtcttatttt aaggatatgg
     2281 agctcaataa ggcacaggag gacatggtat ttgaatttct catcacaccg catgagcagg
     2341 agaatgagag tgcaggcgat tatgaggatg aatcaaatga ggacgaatta catggggatg
     2401 aagcgtctga aaatgctgca ttgaacaata taatttactc agaaggcgat tcgatggatg
     2461 ccatagcagg tgggaaaaat aataaagata atcaggataa tgaagctgag cctgacatag
     2521 atgaggacag tctcataccg gattcaccta tgtttaggat gtatcttgag gagcttgaag
     2581 agatgcctga ttacacgaaa gccgagcagg acgatatgta caaaaagctg ctcgcgggcg
     2641 atgaaagtgt gatacacact atatcaaatt tctggctcaa aagtgtactt gccatggcaa
     2701 agaagcttgc agttacatca gagggctttg aggacgtagt gcaggaagga aatatggggc
     2761 ttttcatgtg ccttaccgag ctctgcggct gcggggatga gatagatgtg gaaaaagagc
     2821 ttctggatgc catagaggag tctatgaaag cttgtatcag agagcagaca ggtgaggctg
     2881 agagcgagaa tactgtggta ggaaaggttt ccttagtaaa ccgtgcagtg gagcagttca
     2941 aatctaataa gggctgtgag ccgagcattg gtgagctttc aggctacacc ggtgtggatg
     3001 aggaggagct tgaaaagctt cttgagctta tcaaaaaggc tgatgtggag aaaaataaac
     3061 aggacagtag ataatctgtg tcatttgtag atttgtgtca tatattaaac catacctgaa
     3121 tatatgaaag gatggaaaat atatggcacg atttactaat caggcacagc ttcgttatgg
     3181 caacaatgta gccaattcaa atatagcagt cggagaaata ctggaggtgc tttctgctac
     3241 gaaaacagca gtcaaaaaca cctacaatca aaacgataca ataacttatg tggtgagtat
     3301 cgtaaactcc ggaaataccg caataaacgg tctgacactt tctgacaatc taggtgctta
     3361 tacctttaac accaacactc ttgtaccatt aacctatgtg aataacaccg taaaatacta
     3421 tacaaacggc acactccagg cagcgcctgc agtaacccag ggaccgcctc ttagcatcac
     3481 aggcataaat gtcccggcag gtggtaatgc aactgtcatc tatgaggcag cgttaaatga
     3541 gtatgctcct cttggcacag aggcatctgt cacaaacacc gctacagtaa gcggcacagg
     3601 aatcactccg gtcacagcag cagagacagt aaatgccgag gctgcaccaa atcttgcaat
     3661 tacaaagtct gtaagccctg ttccagtgac ggaaaacggt acactgacct ataccttcag
     3721 gattcaaaac tacggaagtg ttgcagctac caataccaca ggagtggtta taacagacac
     3781 atttgctccg gttcttagta atcttacagc ggcattaaac ggcacagcct ggactgcagc
     3841 tacggattac acttacaatg aggcgacagg cacattttct agcactgcag gtgcaatcac
     3901 tgttccggcg gcaacctaca cacaggatag tacgacagga gcgtgggttg tcacaccggg
     3961 cgagagcacg cttgtcatca caggaaccgt gtagcagcgg tgattaataa ggtttaagaa
     4021 ttgtttaatt tgagggataa aagttgaaat atttatgcaa actatgtaaa ataaatgtag
     4081 ctgtttatga ataagatgat gaagatgatc atcggcagtt taaaatgatt aaccggggga
     4141 taagacaaat gaataatgca cagttagtga tagacgaagt aaaaaaggca gtaaagggca
     4201 aggatgattg tatcatcaag gcatttgctg cctttttggc aggtggacat atactgcttg
     4261 aggatgtgcc gggagtggga aagactacgc ttgcagtagc
//
LOCUS       DPUF01000197            9613 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4029, whole
            genome shotgun sequence.
ACCESSION   DPUF01000197 DPUF01000000
VERSION     DPUF01000197.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4029"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(20..517)
                     /locus_tag="DHV81_06995"
     CDS             complement(20..517)
                     /locus_tag="DHV81_06995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94203.1"
                     /translation="MDKKAIAEEIENEINASYRSKNLRQLIDILLSVITLKTGEKDLA
                     GIEERMYVSLGKIFDEDTSVSDIKLCLSNVVRIEPLLKKILFLVNEGEYDNVVQKNLG
                     LAHVIGVLGLNPEHKKLDVEPETYVGDYNYMEYIARSYLLRNSESHTYAEWKRKEIYI
                     YIDSV"
     gene            complement(686..1030)
                     /locus_tag="DHV81_07000"
     CDS             complement(686..1030)
                     /locus_tag="DHV81_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI94204.1"
                     /translation="MKRVLFKIIVPVLIFGLWVLTCYPVCNRAEGFDWFLYWILVGCS
                     FCIRRMCLWFVSKNFEFRGRVGAFVLKCIAGGLIGGIVLNFKITDFIGEVISIVLGHF
                     WIKRKRKISING"
     gene            complement(1011..2074)
                     /locus_tag="DHV81_07005"
                     /pseudo
     CDS             complement(1011..2074)
                     /locus_tag="DHV81_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2097..2984)
                     /locus_tag="DHV81_07010"
     CDS             complement(2097..2984)
                     /locus_tag="DHV81_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HCI94205.1"
                     /translation="MSKTGSAAKVKLNSFDDLFGTEQPQTGTEQVQEIALSELYEFKG
                     HPFKVLDDEKMQETMESIREHGVLMPGIARPMKDGGYEIIAGHRRRHACELVGLTTMP
                     MFIRDYTDDEATIIMVDSNIQREDILPSEKAKAYFMKYEAVKHQGKKGKGNSLDEVGE
                     TAGESAKTVQRYIYLAHLSDALLDMVDKKKIGIVQGVELSFLTEQQQEWVQVVLEETV
                     VIISTVQAARLKEYGKSDELTLPMVRLILTEPKPIERKVTIKADKISRYFPEDYSNEQ
                     IENVIYQLLDEWNSSNSEK"
     gene            complement(2977..3759)
                     /locus_tag="DHV81_07015"
     CDS             complement(2977..3759)
                     /locus_tag="DHV81_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCI94206.1"
                     /translation="MCKVIAIANQKGGVGKTTTTSNLGIGLAKQGKNVLLIDADAQGS
                     LTASLGIREPDRLEITLATIMASIINDEEIKPEYGILRHEEGVDFMPGNIELSGLETS
                     LVNVMSRETVLRTYIEQQKDRYDYILIDCMPSLGMITINAFTSADSILIPVQAAYLPV
                     KGLEQLIKTIGKVKRQINPKLEIEGILLTMVDNRTNYARDISNLLIENYGSRVRIFEN
                     SIPMSVRAAEISAEGVSIYKHDLNGKVASAYQSLTEEVLGNE"
     gene            complement(3792..3971)
                     /locus_tag="DHV81_07020"
     CDS             complement(3792..3971)
                     /locus_tag="DHV81_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94207.1"
                     /translation="MLKVRLMGTKNDIVLFQKILQRHPKVEVLEISELYSNKGTNKYY
                     RAYAEVQKSNVKSSR"
     gene            complement(3971..4444)
                     /locus_tag="DHV81_07025"
                     /pseudo
     CDS             complement(3971..4444)
                     /locus_tag="DHV81_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4717..6123)
                     /locus_tag="DHV81_07030"
     CDS             complement(4717..6123)
                     /locus_tag="DHV81_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCI94208.1"
                     /translation="MKKGFRGTGTVERVDFPNKGIAVTDDGDRVIVKNTIPGQKVEFV
                     VNKVKHQRAEGRLMEVIEKSPLETEEPCPHFGMCGGCTYQTVPYEKQLDMKLTQVKKL
                     ISDAIGTEKEAGYEFIGIHGSPKKSEYRNKMEFSFGDEYKDGPLALGMHKRGSFYDLV
                     TVSDCQIVDKDFRTILKATLDYFSKNNIPYFHRATHKGYLRHLLVRKATKTGEIIVDL
                     VTTTQTEGFNEEELLAGFRYALLTRHYDGSFKGVLHTKNDSVADVVKNEGTEVLYGDS
                     YFYEELLGLKFKITPFSFFQTNSLGAEVLYETAREFILGGDRDSLNGKTVYDLYSGTG
                     TIAQLMAPVCKEVVGVEIVEEAVCAAKENAALNGLDNCKFIAGDVLKVLDEIEEKPDY
                     IILDPPRDGIHPKAIGKIIEYGVENMVYISCKPTSLARDLQIFIARGYRVEKICCVDM
                     FPNTYHVETVVGLHRKDM"
     gene            complement(6242..6556)
                     /locus_tag="DHV81_07035"
     CDS             complement(6242..6556)
                     /locus_tag="DHV81_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94209.1"
                     /translation="MSKVEELLKDNKLAEKLSMAKLNDLLKKEEEDKKPSKAVIIFAI
                     IGVLVAVAAAAYGIYRFFTPDYLDDFDDEYDDEFDDDFFEDEDDDDAEEEKEAPKTKE
                     EK"
     gene            complement(6657..8985)
                     /gene="pcrA"
                     /locus_tag="DHV81_07040"
                     /pseudo
     CDS             complement(6657..8985)
                     /gene="pcrA"
                     /locus_tag="DHV81_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742254.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
     assembly_gap    6825..6892
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9159..>9613
                     /locus_tag="DHV81_07045"
     CDS             9159..>9613
                     /locus_tag="DHV81_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94210.1"
                     /translation="MENDVKEFLSELLKQLKSIDYIKPEDIPNIDLYMDQVTTFMDEQ
                     LAASKRYESDKILTKTMINNYAKNNLLPSPDKKKYSKEHVLTLLFIYYFKNILSINDI
                     RSILNPLTEKYFGNDDGFTMQDVYTEVFSLEAEESQKLLKDLGKKYKIAG"
BASE COUNT         2521 a         2390 c         1511 g         3123 t
ORIGIN      
        1 ccttgacaaa ggggacagtt taaacactat caatatatat atatatttcc ttccttttcc
       61 attctgcata tgtatgagac tcactatttc ttaataaata cgaacgtgct atatactcca
      121 tataattata atcgcctaca tatgtttccg gttctacgtc taatttctta tgctctggat
      181 ttagacctaa tacaccaata acatgtgcaa gaccaagatt cttttgtaca acgttgtcgt
      241 attcaccttc attaacaaga aacaatatct tctttaataa cggttcaatt cttaccacat
      301 tagaaagaca taatttaata tcactaacag atgtatcttc atcaaatatc tttcccaaag
      361 aaacatacat tctctcttca atacctgcca aatccttttc tcctgtcttt aatgttataa
      421 cagaaagtaa aatatctata agttgtctta aatttttact tctataactt gcatttatct
      481 cattttcaat ttcttctgca atagcttttt tatccatgat ctgacttcct ttcaaaatct
      541 gtaattttta aagcataatt ccatatcaaa atgtatgcat accatcttgt atccatttcc
      601 attatactct atatcaattt ttcttgcatc catttattct acacagtatt ttataaataa
      661 gaaaatcctt ttgctatttt actttttatc cattaatact tatttttctt ttacgtttta
      721 tccaaaaatg tcctaataca atactaataa cttctcctat gaaatcagtt attttgaaat
      781 tcaacacaat acctccaatc aaacctccag caatacactt caaaacaaaa gcaccgactc
      841 ttcctctgaa ctcgaaattc tttgatacga accaaagaca cattctgcga atacaaaacg
      901 aacatccaac taaaatccaa tataaaaacc aatcaaaacc ttctgctcta ttacataccg
      961 gataacaagt cagcacccac aaaccaaata tcagtaccgg aacgataatc ttaaacaata
     1021 cccttttcat gccagcctcc cccatacata tcatgctgca cttcctgctg ataataatga
     1081 tttattgttg atggcgcatt atacagtgcc gtaaccatgt atgccttaat gtttgaaatt
     1141 tttgttgtcg tgtctttcat acaccccatc acatactcca gatgactgct cctcagcttt
     1201 aagaattttg actttacaag ctcataggga tactcctccc ccgcaatccg tatcgtagtc
     1261 cgctttacac atacaacctc acagatcaat tcaaataatt catcatacag ttccctgtca
     1321 tttatttcat catatttcat gtgatgatcg tactcaatat tttctttgat aatctctatg
     1381 tatgcatctg ctccatccat cctatcatcg gtctgatttc cttcaccgga tggatagatt
     1441 ggattggtat aactcatatc agtataattt atatcagtct tattatagtt agtataaata
     1501 ggggctaaat cctgaacctc tgcaagttca gtttctgaac ctcttgaagt ctgcttttta
     1561 tacttctggt ggttcagatt ctgtacctcc tgaagttcaa tttctgaact tcttggagta
     1621 tcaatattag acttcttgaa gttcaaattt tgaacttctg tggattttcc agtattctca
     1681 ggctcttttt ctcctgttcc gctgacattg tggcaaagtt tttcacatag ataatatccg
     1741 gtttgccaag acctctgcgc tttttttcaa tcaacccaat ccctttttcg gaatccagct
     1801 ctttcataat cttgacacag gttgctttcg cacatcccaa atcttccata atcgcatcca
     1861 ccgtatagat gatatacgcc ctgttttcct cgtctagcca tccatttttc atagacagtg
     1921 acatccgatc gagcatcaag ccatacagga gcttggcatc gctgctaagc cccttgaacc
     1981 gctcatcctt aatcagcaac cgggggacac gataaaaaga gaactggtct gcttcaattc
     2041 catagtagta atcaaatttt gtattctcat tcactttttc atgcctcctt tgctgcttat
     2101 ttctcactgt ttgaactatt ccactcatcc aatagctgat agataacatt ttcaatctgc
     2161 tcattggaat aatcttctgg aaaatatctg ctgatcttgt ctgccttgat ggttactttt
     2221 cgctctatcg gtttcggttc tgtcaggatc agccttacca tcggtaatgt cagttcatca
     2281 ctttttccat actccttcag ccttgctgcc tgtactgttg aaatgatcac cactgtttct
     2341 tcaagaacaa cttgcaccca ctcctgttgc tgttctgtca gaaatgacag ctctacaccc
     2401 tgcacgattc caattttctt tttatcgacc atatctaaaa gtgcatccga taagtgggca
     2461 aggtaaatat atctttgtac tgtttttgca ctctctcctg cagtttcccc aacctcatcc
     2521 agactgttgc cttttccttt tttaccctga tgttttacag cttcatattt cataaaataa
     2581 gcttttgcct tttcactcgg aagaatatct tccctctgaa tattgctgtc caccatgata
     2641 attgtagctt catcatccgt ataatcacgg atgaacatcg gcattgtagt aagtccgaca
     2701 agctcacagg catgtctcct gcgatgtccg gcaataatct catagccgcc atctttcatt
     2761 ggtctggcaa tgcctggcat caatactcca tgctccctga tgctttccat cgtttcctgc
     2821 atcttttcat catccagtac cttaaacggg tgtcctttga actcatacag ttctgacaat
     2881 gcgatttcct gtacctgctc tgttcctgtc tgtggctgct ccgttccaaa taaatcatca
     2941 aagctgttta actttacctt tgcagcactt cctgtcttac tcattgccga gcacctcctc
     3001 cgtcagtgac tggtaggcac ttgctacctt gccattcaga tcgtgcttat aaatgcttac
     3061 accttccgca gaaatttccg cagctcttac tgacattgga atactgtttt caaatatccg
     3121 cactctgctt ccataatttt caatcagcag attgctgatg tctctggcat aattggtacg
     3181 attatcaacc attgtaagca aaattccctc aatctccagt ttcggattga tctgtctctt
     3241 tacttttcca atagttttaa tcagctgttc cagacctttt accggcagat acgctgcttg
     3301 tactggaatc aagatactgt ccgcactggt aaaagcattt atcgtaatca tgccgagtga
     3361 tggcatacag tcaatcagaa tgtaatcata gcggtctttc tgctgttcta tgtaggtacg
     3421 aagcaccgtc tccctgctca taacattcac aagtgaggtt tccaaaccgg aaagctcgat
     3481 atttcccggc atgaaatcca ccccctcctc atgtcttaaa atgccgtact ccggttttat
     3541 ttcctcatca ttgataatgg atgccataat ggttgcaagt gtaatttcca gtctgtccgg
     3601 ctcccgaata ccaagacttg ccgtaagact gccctgtgcg tccgcatcta tcagcaaaac
     3661 attcttcccc tgttttgcca gtcctatccc taaattactt gttgtggtgg tctttccaac
     3721 tccacccttc tgatttgcga ttgctattac tttacacatg ataaattctc ctattctcta
     3781 ctattttgct tttatctgct gctctttaca ttgcttttct gcacttccgc ataagctctg
     3841 taatatttgt ttgtcccttt attgctataa agctctgata tctccagaac ttctactttt
     3901 ggatgacgct gtaagatttt ctgaaacaac acaatgtcat tcttcgttcc catcaatctg
     3961 acttttaaca ttagtctgct tctccaaata aggtatacag ttcccggtta taagtcgcat
     4021 actccggata gcggatctgt atcgcctgcc aaaagaaagg tgcataggca cagcagaaca
     4081 aatcctccac tgtatactgc ctgcactagt ttacctgttc ctctgttttt gtgtaatcat
     4141 ctacactcgg tatgccattg caatacaggt taaatgccat tcgcaccacc ttcatactgc
     4201 cactggtctg ccagccttca tgcagacact cagtttttac acatcccgtc ttaaaatcat
     4261 agatgctatc aacatttctt ctcgtatcat cgctgatacc aaggcaatag caaagtgcct
     4321 tgtggtacac atcctgatag cgtacctctt ttaatttttc atagtagaat ttttcatgtg
     4381 cttcgctgat aaaattgatt gtctgttcct ttttgttttc tgctcctaac gctgtgttgt
     4441 tcatagtgaa cctcctttta aattgaattg aaaaaggcac tcctaatgga atgccctaat
     4501 ataaaaaata gggacactct ttcaagtatc cctacgttct atcaacatat ctgatagttc
     4561 tctatttact ttcatattca gaattctttg tgttcccaca accgaggtct aacgctacaa
     4621 caacagtcac cttcctcaca agctcttttc ccttgtgatt ttcagcaatc ctgttgaagc
     4681 tgaaaaactc tacaaagtgc gtaaattcaa ggatttctac atatcctttc tgtgtagtcc
     4741 taccacagtc tccacatgat aagtattcgg aaacatatcc acgcagcata tcttctccac
     4801 tctatatcct ctggcaataa atatctgcaa atcccttgcc aggcttgtcg gtttacatga
     4861 aatataaacc atattctcaa caccatactc tatgattttc ccgatagcct tcggatggat
     4921 accgtctcgt ggtggatcaa gaattatata atccggtttt tcctctatct catcaagcac
     4981 cttgaggaca tcacctgcga taaatttaca gttatcaaga ccattaagag ctgcattttc
     5041 ctttgctgcg caaactgcct cctcaactat ttcgacacct actacttcct tacacaccgg
     5101 tgccataagc tgcgctatgg tgccggttcc gctgtagagg tcgtagactg ttttaccgtt
     5161 tagcgaatcc ctgtcacctc ccaggatgaa ctctctcgct gtctcataga gcacctccgc
     5221 tccgagtgag ttggtctgga aaaatgagaa tggtgtgatt ttgaatttca gacctaagag
     5281 ctcctcgtag aaatatgaat ctccgtagag cacctctgtg ccctcgtttt tgaccacatc
     5341 tgccacgctg tcattttttg tgtgtaaaac gcccttaaag ctgccgtcat agtgcctggt
     5401 caaaagcgca tatctaaagc ctgcgagcag ctcctcctcg ttgaagccct ctgtctgtgt
     5461 ggtggtgaca aggtcgacta tgatttcacc ggtttttgtt gcctttctga caagcagatg
     5521 ccgcagatag cccttatgag ttgctctgtg gaaatatgga atattgtttt ttgaaaaata
     5581 atcaagtgtt gcctttaata ttgttctgaa atccttatcc actatctgac agtccgatac
     5641 tgtcacaagg tcataaaagc ttcctctttt gtgcatgcca agcgcaagtg ggccatcctt
     5701 gtactcatca ccgaaggaaa attccatctt atttctgtat tcactctttt tcggacttcc
     5761 atgtatccct ataaactcgt atcctgcctc tttttcagtg ccaatggcat ccgaaatgag
     5821 ctttttcacc tgggtaagct tcatatcaag ctgcttttcg taaggcactg tctgatatgt
     5881 acagcccccg cacattccaa agtgaggaca tggctcttct gtctccagag gcgatttctc
     5941 aattacctcc atgagtctac cctctgccct ttgatgcttt actttattta caacaaattc
     6001 caccttctgt cccggaattg tatttttcac aattactcta tcgccatcgt ctgtaacggc
     6061 gattcccttg tttgggaagt ctactctttc tacggttcct gttcctctaa atcctttttt
     6121 catgtaattc ttatctctct ttatacttta ttattccttt gtatgcctga ctgcaaatgc
     6181 cttgcttcac acataaagaa aaatgaggac tacatatgta gcccccatta tctaaaaacg
     6241 attatttctc ttctttagtc tttggtgcct ctttttcctc ttcagcatca tcatcgtctt
     6301 catcctcgaa gaaatcatca tcgaattcat catcatactc atcatcgaaa tcatctaaat
     6361 aatccggtgt aaaaaatctg tagataccat atgccgctgc tgcaactgca actaatacac
     6421 caatgatggc aaatatgata acagccttgg aaggcttctt gtcttcctct tcttttttga
     6481 gaaggtcatt aagctttgcc atgctgagct tctctgcaag cttgttgtcc tttaataatt
     6541 cttcaacttt actcataatt caatccgctc cttttccaaa agtaattgtc aaatgctatt
     6601 tgttaaatcc attatagcat attatatcca attttcctat aaaaattaca ataattttag
     6661 tcaatttttt tcaatttggc aaatgaagca aacatctttt tggtaccggc cttgtcaaag
     6721 tccacagtga cctcataatc cctgccgcct tctacaattg ccatgacctc tcccgcgcca
     6781 aatttaatgt gtgacactct gtctcctacc ttatatgaaa ggccnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgctcac
     6901 aggcgtgtag acaggtgtcc ttgtacgctg tgtattaaag gtggtcgcat actctgtgcc
     6961 gtatacaggt gtctttttga aggcctcctt agcctttctg tacgtgctct gtgaatttct
     7021 ttcaaggtca tcatcacggc gctttggctc atagacctca cctgacataa gctcacgcgg
     7081 aatctctttc acaaatggcg ataccttgtt aaaccgtgtc tctccacgca cattgcgcat
     7141 acgcgcaaat gtgatggtaa gatgctcctt ggctctcgtg atgccaacat aagcaagacg
     7201 cctttcctcc tctaactcgc ttgtctcatc atcactcgtg tttgagaacg cgcgacatgg
     7261 gaaaaggcca tcctccatac ctgccagata cacattcgga aactcaagtc ccttagcact
     7321 gtgcagagtc ataagaacca catagtcgct gttttcgtca agaccgtcta tatcggctac
     7381 aagcgctacc tcctccaaaa agccactgag agtaggattc tcctccccct gctcgtaatc
     7441 aacagccttg tttataagct catcaatatt ctcaatacgc tgctctgctt catccgtgcc
     7501 ctctgccttc agctcatcga catagccggt ctcatcgatt attgcctgta tgagctgcga
     7561 aaccgaatcc atctcggctc tcgcacgaag tgactgtata aacatcacaa aaggctttat
     7621 cttgcctgct gacctcgcca tgcccggcac ctcatcgaca tagcgcagtg ccgtatagaa
     7681 atctattccc ttctcaatgg catagtctgt caccttattg atgcttgtag ctccgatgcc
     7741 gcgctttggc acatttatta ttcgctttac cgcaaggtca tcatggccat tgtcaatagt
     7801 ctttaaatat gcaaggatat cctttatctc ttttctggca tagaaattca cgccgccgac
     7861 aatcttgtat ggtatgttgg ctgcaataaa tttctcctcg aagagacgcg actgtgcgtt
     7921 ggttcggtaa agcacagcac agtccttata gcgcgcctca ccctttctca cgagtctcgc
     7981 tatcgaacgc gccacataat ccgcttcctc gaagccactc tcaagctgtt caaaggtaat
     8041 cctgtctccg gctccgttgt cagtccaaag gcgcttgtcc tttctaccca cgttgttgga
     8101 aatgacgctg ttggcggcat ctaggatatt ctgggtagac ctgtaattct gctcaagctt
     8161 tatgaccttt gcatcttcaa aatgcttctc aaagtttaag atattgtaga tattggcacc
     8221 tctgaacttg taaatggact gatcatcatc acccaccaca cacagattct tatacttcat
     8281 ggcaagaagc ctgataagct caaactgcac ggtgtttgtg tcctgatact catccaccat
     8341 gatatatctg aagcgctcct gatagtaatc cagcacctct ttatccagct taaaaagctc
     8401 tactgtcttc acaagcaaat catcaaaatc gagtgcgtta ttctgcttta gggcattctg
     8461 atactccttg tatacaagtg cctgcttgcg cttcacgaag tcacctgtga agcggttttc
     8521 aaactctatc ggatcaatca tctcatcctt tgcagatgaa atgacattta aaaacatctt
     8581 ttccttatac atcttggtat ctatctcaag acgcttgcag atgtccttca tcagagcctt
     8641 ctggtcgtca gcatcatata tggtaaaatt cgtgtcatat cccagcctgt caatatacct
     8701 gcgcagtata cgcacacatg tcgaatggaa tgtagaaacc catatgcttt ccgaaccata
     8761 gcccaccata tcatcaattc tctcacgcat ctctcccgca gccttgtttg taaaggtgat
     8821 tgccatgata ttgtaaggat tgacgccaca ctcatctatc aggtaggcag tcctgtatgt
     8881 aagaaccctg gtcttgccac tgcctgcccc cgctaaaata agaagcggtc cctctgtggt
     8941 caaaacggcc tccctttgct gttcattcaa catatctaag ttcatctgtt ttcctctcaa
     9001 ttctccagtc ttttacaggc acacatacca taaatacaaa aataatttat ttacagcata
     9061 tcacaaacat atgttttgta caacccaata tgagaaaatt caaaaaaaac ttgtcatata
     9121 gttttcaata ctatataata aaagcctgag gtgatgatat ggagaatgac gttaaagaat
     9181 ttttatcaga attattaaag cagcttaaat caatagatta cataaagccg gaggatatac
     9241 caaatattga tttgtacatg gatcaggtca caaccttcat ggacgagcag ctcgctgcat
     9301 caaagcgcta tgaaagcgac aaaatcctta ccaagacaat gataaacaac tatgccaaaa
     9361 acaatctgct cccttctccg gacaagaaaa agtattctaa ggagcatgtg ctcacactgc
     9421 tttttatata ttatttcaaa aatatattga gcatcaacga tatccgctca attcttaatc
     9481 cgcttaccga gaagtatttc ggcaatgatg acggatttac gatgcaggat gtctatactg
     9541 aggttttcag cttagaggcg gaggaatcgc aaaagctcct aaaggatctc ggcaagaaat
     9601 acaagattgc cgg
//
LOCUS       DPUF01000198            3488 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_36409, whole
            genome shotgun sequence.
ACCESSION   DPUF01000198 DPUF01000000
VERSION     DPUF01000198.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_36409"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="DHV81_07050"
     CDS             complement(<1..1171)
                     /locus_tag="DHV81_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit methyltransferase I"
                     /protein_id="HCI94211.1"
                     /translation="MENHTIVTLKKGEGRTIKAGGAWIFDNEIDTITGTFTNGDIVVV
                     HDFDGYPMGRGFINQNSKIRIRMMTRKADQLIDDNFLRMRVQNAWDYRKQVMAGGNDN
                     VPDLNCCRVIFGEADFLPGITVDKYADVLVVECLALGMEQFKVKIVELLKEVLAADGI
                     NIRGVYERSDANERKKEGLPKVKGFIGAEFDTNVEIVENAVHYMVDVENGQKTGFFLD
                     QKYNRLAMQRICKGKRVLDCFTHMGTFALNAGIAGASEVTGLDISEYAVKQATENAKR
                     NNLSDTVKFRVANVLDELPRLAADGEKYDVVILDPPAFTKSREATKNAIKGYREINMK
                     GLKLVKDGGFLATCSCSHFMTQELLAKTVKEAAKATHKRLRQVEFRTQGPDHPILW"
     gene            1402..2493
                     /locus_tag="DHV81_07055"
     CDS             1402..2493
                     /locus_tag="DHV81_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI94212.1"
                     /translation="MITISLCMIVKNEEEVLERCLNSLKGLMDEIIIVDTGSTDSTKE
                     IAARYTDKIYDFSWCDDFAAARNFSFSKATQEYIYAPDADEVLDDTNRRRFMMLKAAL
                     LPEIEIVQMKYITPSKFNTVQNSSSEYRPKLFKRLRTFTWINPVHETVRLEPVVYDSD
                     ICIQHLPQGTHGKRDFTIFKRSFEKNGTLPKSIATMYAKELLKCGSPEDFTNALPYFQ
                     GEYRNSPDIESTCVLSRYYRMNGDYDKFFSVALKNVAVDGCSEVCCELGAYYFDKADY
                     EEASLWYYNAAFETKPVLDVECGGGKALHALSECYSRWADEKQKKLDSLPPKSRNVFK
                     GDKELIDSLRSQAADYQKQAEEWMLPEGD"
     gene            2638..>3488
                     /locus_tag="DHV81_07060"
     CDS             2638..>3488
                     /locus_tag="DHV81_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyryl-CoA:acetate CoA-transferase"
                     /protein_id="HCI94213.1"
                     /translation="MSFLEEYKQKLVSADEAVKIVKSGDWVDYGWCTGTPDALDKALA
                     RRTDELKDVNVRGGILLKPLAIFEREDAGEHFTWNSWHMGGYERKLIKRGCSFYSPIR
                     YSELPRYYRDSTTPDDVAMFQVAPMDSHGYFNFGPNASHLGAVCETSKKIIVEVNENM
                     PRCHGGSEANVHISQVSYIVEGDNPAIGELGAGGPATDVDKKVAELIVDQIPNGACLQ
                     LGIGGMPNAVGSLIAESDLKDLGVHTEMYVDAFVDIAKAGKITGAKKNIDRYRQAYGF
                     GAGTKKM"
BASE COUNT          930 a          832 c          758 g          968 t
ORIGIN      
        1 cccacagtat cggatgatcc ggtccctgtg tgcggaactc cacctggcgc agcctcttgt
       61 gagttgcctt tgccgcttcc ttgaccgttt ttgcaagcag ctcctgcgtc atgaaatgag
      121 agcatgagca ggtagccaga aatccaccat ccttaacaag cttaagtccc ttcatgttaa
      181 tctcgcggta gccctttatc gcatttttgg tagcctcacg cgactttgta aatgccggag
      241 gatccagaat gactacatca tatttttcac catctgcagc gagccgtggc agctcatcca
      301 gcacatttgc cacgcgaaac ttcactgtgt ctgagagatt atttctcttc gcattttctg
      361 tagcctgctt aaccgcatac tctgatatat caagtcctgt gacttcgctt gcccctgcaa
      421 ttcctgcatt gagggcaaat gtgcccatgt gcgtgaaaca gtcgagcact cttttcccct
      481 tgcagatacg ctgcatagca agcctgttat acttctggtc taagaagaag cctgttttct
      541 gtccattttc cacatcgacc atatagtgaa ctgcgttttc tactatctcc acatttgtgt
      601 caaactcggc tcctataaag ccttttacct taggaagacc ttcttttttg cgctcatttg
      661 catcactgcg ctcgtatact ccacgaatat ttattccgtc cgccgccagc acttccttta
      721 acagttcaac gattttcacc ttaaactgct ccattccaag tgccagacac tccaccacaa
      781 gcacatctgc gtatttgtcc actgtgatac ccggcaggaa atcagcctcg ccaaatatca
      841 cacgacagca gtttaaatca ggcacattat cattgcctcc tgccatcacc tgcttgcgat
      901 aatcccaggc attttgcact ctcatcctga ggaaattgtc atcaatcagc tggtctgctt
      961 ttcttgtcat cattctgata cggatttttg aattctggtt tatgaagccg cgccccatcg
     1021 gatatccgtc gaaatcgtgc accacaacaa tgtctccgtt cgtaaatgtg cctgtgattg
     1081 tgtctatttc attgtcaaaa atccatgcgc cgcctgcttt tatagtgcgt ccttcgcctt
     1141 ttttcagtgt tacaattgta tgattttcca tatttgttcc tcttctaaat tacttcatta
     1201 ttccttgcta aaatctgatt atatagttta tctgaaattc tatagccttt agaaataagt
     1261 ttatctgtcg acattccatt tcaattatat gaaatcccgg caatatttac aatacacaaa
     1321 tatttctgca aaacatgccg atatatatat tataatataa tcgtaactat tataacatcc
     1381 gaccggtaaa tggagataaa tatgattaca attagcttat gcatgatagt aaaaaatgaa
     1441 gaagaggtgc tcgaacgatg cctgaacagt ctcaaagggc tgatggatga aatcatcata
     1501 gtggacaccg gctcgacgga cagcacaaag gagatagctg cacggtatac ggacaaaatc
     1561 tatgatttct catggtgcga tgattttgct gcggccagga atttttcgtt ttccaaggct
     1621 acgcaggaat acatatatgc acctgatgcc gacgaggtgc tcgacgatac caaccgcagg
     1681 cgtttcatga tgcttaaggc tgcgctgctg ccggaaattg agattgtcca gatgaaatac
     1741 attactccaa gcaaattcaa cacagtgcaa aattcatcct cagagtatcg cccgaagctg
     1801 ttcaagaggc ttcgcacgtt tacatggatt aatccggtgc acgaaacggt tcgcttagag
     1861 cctgtagtct atgacagcga tatctgtata caacatctgc cgcagggcac tcacggcaag
     1921 cgtgatttta ccatattcaa gcgctccttt gaaaaaaacg gcaccctgcc aaaaagtata
     1981 gcgactatgt atgccaagga gcttttaaaa tgcggaagcc ccgaggattt taccaatgcc
     2041 ctgccatatt ttcagggtga ataccgcaac agccctgaca tcgaatccac ctgcgtgcta
     2101 tcacgctact atcgcatgaa tggcgactat gacaaatttt tttctgttgc cctaaaaaat
     2161 gtagcagttg atggctgcag cgaggtgtgc tgtgagctcg gtgcgtacta tttcgacaag
     2221 gcagactatg aggaggcaag cctgtggtat tacaacgctg ccttcgagac aaagccagta
     2281 ctcgatgtgg agtgcggcgg tggcaaggcg cttcatgccc tgtctgagtg ctactcaaga
     2341 tgggctgatg agaagcagaa aaagcttgat tcgcttcctc ctaaatcaag gaatgtattc
     2401 aaaggcgata aggagctgat tgattcactg cgcagccagg cggccgatta ccaaaagcag
     2461 gctgaggaat ggatgcttcc ggagggtgac tagcagaggc tgtcatataa aagtctctac
     2521 cagcatttac caacttgtaa aatcctctct aaagtgataa tatagaagtt atagagtttg
     2581 ttattttttt aacgtagcat tttgctacta tcattttagg tatctaagga ggattttatg
     2641 agctttttag aagagtacaa gcagaaactt gtctcagcag atgaggcagt aaagattgtc
     2701 aaatcaggcg actgggttga ctacggctgg tgcacaggca cacctgatgc actcgacaag
     2761 gcacttgccc gaagaacaga tgaacttaag gatgtcaatg tacgtggtgg tattttgctt
     2821 aaaccacttg ccattttcga gcgtgaggat gccggcgagc atttcacatg gaactcatgg
     2881 catatgggcg gttatgagag aaagcttatc aagagaggct gttctttcta ctcaccaatc
     2941 cgttactcag agcttcctcg ttattataga gattcaacaa cacctgacga tgtcgctatg
     3001 ttccaggttg caccgatgga ttcacacgga tacttcaact ttggtcctaa cgcatctcac
     3061 ctcggtgctg tatgcgagac ttcaaagaaa atcatcgttg aggtcaatga aaacatgcct
     3121 cgctgtcacg gcggttcaga ggccaatgta catatttcac aggtttctta cattgttgag
     3181 ggtgacaacc ctgcaatcgg agagcttggt gccggtggtc ctgcaacaga tgtagacaag
     3241 aaggttgcag agcttatcgt tgaccagatt ccaaacggtg cctgtctcca gctcggtatc
     3301 ggcggaatgc caaatgcagt aggctcactc atcgctgagt cagacctcaa ggatctcggt
     3361 gtccacacag agatgtacgt tgacgcattc gttgacatcg caaaggccgg aaaaatcaca
     3421 ggagcaaaga aaaacatcga ccgttatcgt caggcttacg gcttcggtgc cggaaccaag
     3481 aagatgta
//
LOCUS       DPUF01000199            5051 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_15184, whole
            genome shotgun sequence.
ACCESSION   DPUF01000199 DPUF01000000
VERSION     DPUF01000199.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_15184"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>656)
                     /locus_tag="DHV81_07065"
     CDS             complement(<1..>656)
                     /locus_tag="DHV81_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-L-homoserine sulfhydrolase"
                     /protein_id="HCI94214.1"
                     /translation="TKLEGGIASVACASGMAALMNTFLNILSSGDELISSASLYGGTI
                     DLFHDLEAFGITTRYVENNNWQQIEDSINEHTRLIFAETIGNPCLDVTDIEMLAEIAH
                     AHDIPLVVDNTTSTAYLIQVLKHGADIVVNSSSKYINGSSNAISGILTDSGKFKWDRN
                     RYPGFADYVKYGPMAFVAKLRNSLFRNMGACLAPVNAYLNIIGLETLGLRMERECSNA
                     L"
     assembly_gap    657..795
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1001..1978)
                     /locus_tag="DHV81_07070"
     CDS             complement(1001..1978)
                     /locus_tag="DHV81_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCI94215.1"
                     /translation="MFDIIIIGSGPAGLSAAIYAKRANLNVAVAEKEYEGTGQIAESG
                     NVNNYLGLPNINGYDLGEKFREHAVSLDVEFIEKEAVQIEAVQNGEKEESAIYRVKFD
                     DDTIAEARALIYTAGAYPRKAGVPGEDEYTGKGVSYCAICDGAFYKGKTAAVLGGGDT
                     ALDDALYLSDICEKVYLVHRRDSFRGAQSTVELLKQKENVELVLNETVTEIYGEKKPT
                     GIKLKSGRTLAVDGVFVAYGSVPQSELIKNLVQLDERGYVVAGEDGITYAATKNGDHD
                     RSVQPGLYVAGDVRTKTLRQVVTAVSDGANAATTAAEYLRDINGYKQNI"
     gene            complement(2108..3733)
                     /locus_tag="DHV81_07075"
     CDS             complement(2108..3733)
                     /locus_tag="DHV81_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucohydrolase"
                     /protein_id="HCI94216.1"
                     /translation="MNKNNNEKWWKKAVVYQIYPKSFQDSNGDGFGDLQGIIKRLDYL
                     ETLGINAIWLSPVFKSPQADNGYDISDYRDIDPTFGSLNDMEELINEAKKHNIRIMMD
                     LVLNHSSNEHRWFKEAKKSKDNPYHDYYIWRDGDEGVPPSDMKACFGGSAWEYVPEIG
                     QYYFHQFLPEQPDLNWENPKVRRAIYDMILWWMDKGVGGFRLDVIDQIAKEPDKRITI
                     NGPRLQEYFKELSRETFQKGDLITVGEAWGADTERAKLYSNPDGSEFSMVFQFEHIGL
                     DQKEGGEKWDLAPLPFKKLKKIMAHWQNELYNCGWNSLFWDNHDLPRIVSRWGNDREY
                     RVESAKMLAILLHGMQGTPYIYQGEELGMTNVQYDIEDYKDCEIINMYHERLEKGYSK
                     DEIMKSIYAKGRDNARTPMQWDDSANAGFTTGTPWIKVNDNYDKINAKSQVNDPDSIF
                     SCYKKLVQLRKDYPVFVDGKFTLLLEDDENIFAYSRKNEEKTMIVVCNFFDKEIPMPL
                     AKECEGMEVLISNYKDTSDMSVLRPYEARMYIR"
     assembly_gap    4007..4108
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4109..>5051)
                     /locus_tag="DHV81_07080"
     CDS             complement(<4109..>5051)
                     /locus_tag="DHV81_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucohydrolase"
                     /protein_id="HCI94217.1"
                     /translation="MFAKEQPDLNWENEKLRQKLYEMINWWLDKGLSGFRIDAIINIK
                     KNLDFPDFEPDAEDGLAACYKMVESAEGVGEFLEELKNNTFKKYDAFTVGEVFNMKPE
                     ELPEFIGENGHFSTIFDFCAQCLSDGEHGWYDAPKIDFDTWRKAILSSQLETEKYGFK
                     ANIIENHDEPRGASRFLPEYAQNPAGIKMLGTVSVLLRGIPFIYQGQEIGMQNAKWNS
                     IDEYDDISTKDQYKAALAAGLSKEQALAACGRMSRDNARTPMQWSDEANAGFTTAKPW
                     LKVNENYKAINVAAQEKDPSSVLNYYRRLVALRKSDEF"
BASE COUNT         1137 a         1251 c          842 g         1580 t
ORIGIN      
        1 agtgcattag agcattctct ttccattcta agaccgagtg tctcaagtcc tataatattt
       61 aagtatgcat tcaccggagc taaacaggca cccatatttc tgaaaagact gtttctcagc
      121 tttgcgacaa atgccatagg tccatacttc acataatctg caaaacccgg atatctgttc
      181 ctgtcccatt tgaacttacc tgagtctgtc agaataccac tgattgcatt gctgcttcca
      241 ttgatatatt tggaagatga attgaccacg atatcagctc catgctttaa aacctgtatc
      301 aggtaagctg tcgatgtggt attgtcaact acaagcggta tatcatgtgc gtgtgctatc
      361 tctgcaagca tctcaatatc tgtgacatcc agacagggat taccgatagt ctctgcaaaa
      421 atcagtctgg tatgctcatt gatgctgtcc tctatctgct gccagttgtt gttctcgaca
      481 tatctggttg tgatgccgaa ggcctcaaga tcatggaaca aatctattgt gccaccgtag
      541 aggctggcgc ttgagatgag ctcatctcca ctgcttagta tatttaagaa ggtattcatc
      601 agtgccgcca tgcctgatgc gcatgccacc gatgctattc ctccctcgag ctttgtnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnncttgt ctgatatacc ggtggcagtg tagagccatt tgtctctttt
      841 gtcacgccgg catgaagtaa gcttgtatta aatctcattg ttttcttctt ctcgttaatt
      901 cttacatagt ctattgtgtt actaggaatt gatgagatta ttatacaaac gtgtgtattg
      961 aaagtcaatg ctatcagtta tacaaattat ttgtccgtgg ttatatattt tgtttatagc
     1021 cattgatgtc ccttaaatac tctgcggctg tcgttgcggc atttgcgccg tcagatactg
     1081 cggttaccac ctgacgcagt gtcttggtac ggacatctcc cgccacatac agtcccggct
     1141 ggacgcttct atcatggtca ccattttttg ttgcagcata tgtgatgccg tcctcacctg
     1201 ccactacata tcctctctca tcgagctgca ccagattctt tataagctct gactgcggta
     1261 ctgagccata tgccacgaat actccgtcta cagccagtgt cctgccactt ttaagtttta
     1321 tgcctgtcgg ctttttttct ccatatattt ctgtcactgt ttcgtttagg acaagctcta
     1381 cattttcttt ctgctttaag agctcaactg tgctctgtgc acctcggaag ctgtctcttc
     1441 tgtgaaccag atagaccttc tcacatatat cggacagata taatgcatca tccaaagcgg
     1501 tatctccgcc gcccagcact gcggctgtct ttcctttata gaatgctccg tcacagattg
     1561 cgcagtaaga tactccctta ccggtatact catcctctcc cggcactcct gcctttctcg
     1621 gatatgctcc tgcggtatag atgagcgctc ttgcctctgc tatcgtgtca tcatcgaatt
     1681 tcactctgta tatcgctgat tcttctttct caccattttg cactgcttct atctgcactg
     1741 cttctttttc gataaattct acatcaaggg acactgcatg ctccctgaat ttttctccca
     1801 agtcgtatcc atttatgttt ggaagcccca gatagttgtt cacatttcca ctctcggcta
     1861 tctggcctgt cccctcgtat tctttttctg ctactgccac attcagattg gctctctttg
     1921 catatattgc tgcagacagt cctgccggac cactgcctat tattattata tcaaacatgg
     1981 aattggttcc tctcatcgtc cattgaattt tttcggtaac ttattatata cattatcctt
     2041 aaaaaataca ataataccgc ctgcttttat aaaacacctt tataaaaaca agcggtatca
     2101 attttatcta tcgtatatac atcctggcct cataaggtct gagcactgac atatcagagg
     2161 tatccttata atttgaaatc agcacctcca tgccctcaca ctcctttgca agcggcattg
     2221 gtatctcctt atcaaagaaa ttgcacacaa caatcatggt tttttcttca ttctttctgc
     2281 tgtatgcaaa aatattctca tcatcctcaa gtaaaagtgt gaatttacca tcaacaaata
     2341 ccggataatc ctttctcagc tgaacaagct tcttgtagca gctaaagatt gaatcagggt
     2401 cattcacctg tgatttggca tttattttgt cgtaattgtc atttaccttt atccaaggtg
     2461 ttcctgtggt gaagccggca tttgccgagt catcccactg cataggtgtc cttgcattgt
     2521 cacggccctt tgcgtaaatg gacttcatga tttcatcctt tgagtatcct ttttcaaggc
     2581 gctcatggta catgtttatg atttcacagt ccttataatc ctcgatatca tactgtacat
     2641 ttgtcatgcc cagctcttca ccctgataga tgtatggtgt accctgcatg ccgtgcagca
     2701 atatagcaag catctttgca gattccactc tgtactccct gtcattgccc catcttgaca
     2761 cgatacgcgg caggtcatga ttgtcccaga acagactgtt ccagccacag ttgtagagct
     2821 cattctgcca gtgagccatt atctttttga gctttttaaa cggaagcggt gcaaggtccc
     2881 atttctcgcc gccctctttc tggtcaagcc ctatgtgctc aaactggaac accatagaaa
     2941 actcgctgcc atccggattg ctgtaaagct ttgcgcgctc tgtgtctgcg ccccatgcct
     3001 cacccacggt aatcaggtcg cccttctgga aggtctccct gctcagttcc ttaaaatatt
     3061 cctgaagcct tggtccgttt atggtgattc tcttgtccgg ctcttttgct atctggtcga
     3121 ttacatcaag tctgaagccg ccgactccct tgtccatcca ccagagaatc atatcgtaga
     3181 ttgctcttct gacctttgga ttctcccagt ttaaatccgg ctgctccggt aaaaactgat
     3241 ggaaataata ctgtccgatt tccggcacat attcccatgc gcttccaccg aaacaagcct
     3301 tcatatcact tggcggcacg ccttcatctc cgtctctcca aatatagtag tcatggtatg
     3361 gattgtcctt gctcttttta gcctccttga accacctgtg ctcgtttgaa gaatgattta
     3421 aaacaagatc catcataatc cttatattgt gcttctttgc ctcgtttatg agctcctcca
     3481 tatcattgag actgccaaac gtcggatcaa tatccctgta atccgaaatg tcataaccgt
     3541 tgtccgcctg cggtgatttg aataccggcg aaagccagat tgcattgatt ccaagcgtct
     3601 caagataatc aagtcttttg ataataccct gtaaatcgcc aaagccgtct ccgttagaat
     3661 cctgaaagct ctttggataa atctggtaaa ctaccgcctt tttccaccat ttttcattat
     3721 tgtttttgtt cattttgccc tccttatctc ttcattatct cttttagtta tctcttttag
     3781 ctgtctcttt ttgtaaaaat tcagcctagt tactttaatg ctgtatcaaa ttatgctatt
     3841 ttacaagcac caccaaaacc tcacagcttt caagcttaag ctcggatgct cccactgaaa
     3901 cagtgctctt ttcagcatca tttgtgttgg acaaaagcac ctttttcaca ctgccatcca
     3961 gcctgatttg tgcctcttta ctgccaaaat ttgccacaac cagcacnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta aattcatctg actttctgag tgccacaagc
     4141 cttctgtaat agttcaaaac agatgaagga tccttttcct gcgccgcaac attgatagcc
     4201 ttatagtttt cattgacctt aagccatggc tttgctgtgg taaaaccggc atttgcctca
     4261 tcactccact gcataggtgt ccttgcatta tccctgctca ttcttccaca ggctgcaagt
     4321 gcctgctcct ttgacaagcc tgctgcaagt gcggccttgt actgatcctt tgtgctgata
     4381 tcatcatact catctatgct gttccatttt gcattttgca taccgatttc ctgtccctgg
     4441 tatataaacg gaatacctcg aagcagtaca ctgacggttc caagcatctt gattccggca
     4501 ggattttgtg catactcagg taaaaatctt gatgcgcctc tcggctcatc atgattttct
     4561 ataatattgg ccttaaagcc gtacttttct gtctcaagct gtgagctgag aatggccttt
     4621 ctccatgtgt caaaatcaat ttttggagca tcataccagc catgctctcc atcacttaag
     4681 cactgtgcac aaaaatcaaa tatcgttgaa aaatgcccat tctcaccgat aaactccggc
     4741 agctcctccg gcttcatatt gaatacctca ccgaccgtaa atgcatcata cttcttaaat
     4801 gtattgttct ttaattcctc caaaaactct ccgacgccct ctgcactctc aaccatcttg
     4861 tagcaggcag ccagtccgtc ctctgcatcc ggctcaaaat cagggaaatc gagatttttc
     4921 tttatattta ttatcgcatc tattctaaat cctgacaagc ccttatctag ccaccagtta
     4981 atcatctcat aaagcttttg tcttagcttc tcattctccc agtttaaatc cggctgctcc
     5041 ttcgcaaaca t
//
LOCUS       DPUF01000200           25325 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3845, whole
            genome shotgun sequence.
ACCESSION   DPUF01000200 DPUF01000000
VERSION     DPUF01000200.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 25325)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25325)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25325
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3845"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="DHV81_07085"
     CDS             complement(<1..1496)
                     /locus_tag="DHV81_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HCI94218.1"
                     /translation="MDFRAEYEKWCTDPYFDEATKAELKAIAGDDKEIEDRFYRTLEF
                     GTAGLRGVIGAGTNRMNIYTVRQATQGLANYILSQGEDAVKRGVAIAHDSRNMHDEFT
                     DATALCLAANGIKTYVFPQLAPVPELSYAVRTLGTIAGVVITASHNPREYNGYKVYWE
                     DGAQVTPPHDTSIMAEVANVTSFDQVKTMDKAAAIEAGLYNVISDEVEEGYFGELRKL
                     SIHPEIIKAMAKDIKIVYTPLHGTGLRPVQRVLKDMGFENVYIEPSQAVPDGNFPTCP
                     YPNPENPDAWKLALELAKEKDADIVLATDPDADRLGVYCKDTKTGEYVTFTGNMSAML
                     IGEYILSQKSANGTLPENPAFVESIVSTDMGKAIATAYGVKHIEVLTGFKYIGEQMLK
                     FEKTGCNNYVFGMEESYGCLPGTYARDKDAPAAVCMLCEVAAFYKSQGKTLWDGMIDM
                     YEKYGYYREGISTMTLKGIDGAAQINEIMTKARAAEPKKFGDYQVLAIR"
     gene            complement(1584..2411)
                     /locus_tag="DHV81_07090"
     CDS             complement(1584..2411)
                     /locus_tag="DHV81_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCI94219.1"
                     /translation="MNKVKFSVLLSIYYKEKPEYFRECMESIYSQTVLPDEIVLVEDG
                     RLTEELYEAIRDYECRPSEISFVTVKLEKNSGLGLALAEGIKHCSNELVARMDTDDIC
                     VPDRFERQLNAFSENPGLDIIGGYISEFSDDTRNILSERTVPLGNYVIKQYQRKRDAF
                     NHMTVMYKRSAVLAAGNYQDCLLMEDSLLWANMIRNHCRMANIGETLVYARTGDDMLK
                     RRGGFDYFLKYKAGRKRILKTGTISAADYFVTVAAQFVVCMMPLWMRSLVFKKILRN"
     gene            complement(2438..3127)
                     /locus_tag="DHV81_07095"
     CDS             complement(2438..3127)
                     /locus_tag="DHV81_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCI94220.1"
                     /translation="MSRWRTWGEIPAFMKNDFTLKYYRHLSRKRTSMVFKRIFDVIMS
                     AVLLVLLSPVILVLAVWIKVDSRGPVFFRQERITQYGRKFRIFKFRTMVNDADKKGSL
                     VTVGGDSRITRVGAVIRKYRLDEIPQLINVLCGDMSFVGTRPEVEKYVMAYTPAMMAT
                     LLMPAGITSEASIRYKDEDRLLEAADDVDYTYIHKVLPGKMRYNLKYLKRFSLINDIR
                     LCIETVLAVIH"
     gene            complement(3120..4337)
                     /locus_tag="DHV81_07100"
     CDS             complement(3120..4337)
                     /locus_tag="DHV81_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCI94221.1"
                     /translation="MEKRQISFSPPDITEAEVSAVADALRSGWITTGPRTKEFEKRIA
                     KLCGTDKAVALGSATACLESILRFLGIGAGDEVITSAYTYTASASPAVHVGATLKFID
                     VAPDSFEMDYDALEAAITEKTKVIVPVDIAGVPCDYDRIFDIVERKKALFHPSNDVQR
                     AIGRVIVAADSAHAFGASYMRAGECIMCGNVADFTSFSFHAVKNLTTAEGGALTWRPI
                     EGFDDEYIYKQFMLLSLHGQTKDALHKNGLGNWEYDIVAPSYKCNMTDIQAAIGLVQL
                     DRYEGLLKRRRDIIERYDEAFKPLGLLTVPHFTDRHTSSGHLYLFRIPGIGVDRRNAL
                     IVQMAERGIATNVHFKPLPMMTAYKNMGFDIADFPNAYHQYENEITLPLNTKLSDEDV
                     EYIINNLLEILHE"
     gene            complement(4417..>4629)
                     /locus_tag="DHV81_07105"
     CDS             complement(4417..>4629)
                     /locus_tag="DHV81_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HCI94222.1"
                     /translation="EKLYEEMLMSEEGLKETPNKLIHVGEPIEMDDELFEKQLEMLEK
                     ACTSEAKDIKQIVASVVKTYHPDLEN"
     assembly_gap    4630..4752
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4753..6368)
                     /locus_tag="DHV81_07110"
     CDS             complement(<4753..6368)
                     /locus_tag="DHV81_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HCI94223.1"
                     /translation="MSNRLDMKKDTNNRKDKTKYDGPEKPDAYMGNDKQWVFRALVLA
                     FVDMCIIAISFFFALMIRFEFSYSHIPLSFLEGYLHIVPLFMVISLVVFYIFRLYHSI
                     WRFASINEFMHIIGAFLVLAVVSVTIHEVYEIKMPYAFWIIGLMLSFALCTACRFAYR
                     FFRTGQKMLEQRGAANGDEKVMIIGAGNAGRMLIRELIMSSHLHTKACCIIDDNPAKL
                     GRFIDGVPIVGNRNDIPEMVEKYDITKIVYAVPSTSGKDRKDILNICKDTGCDVSVVP
                     GIYQMVDGRVSVSNLRPVSIEDLLGRDPVVVDNDGIHSFLQGKVVMVTGGGGSIGSEL
                     CRQIAREDPKLLIIFDIYENNAYDIQQELKRKYPYVRVETLIGSVRNTNRLHYVFQTY
                     HPEIIYHAAAHKHVPLMEESPNEAIKNNVLGTLKLSRIAAEYHVKKFVLISTDKAVNP
                     TNIMGASKRLCEMVIQMMNRQTETDFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVT
                     DKRIIRYFMTIPEAVALVLQAGLYAHGGEI"
     gene            complement(6519..6938)
                     /locus_tag="DHV81_07115"
     CDS             complement(6519..6938)
                     /locus_tag="DHV81_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCI94224.1"
                     /translation="MDNIIIRNRKEEFKELVLYTFFGNMTFVISIGSYAIFNVFFGIN
                     ELIANALAWVFAVLFSYVTNKKWVFKAATPTKTAFLVQMFAFFSGRFITLVIEETIIF
                     IFITLLDYPSMWVKLAAQVVVVVLNYVISKLFVFKNR"
     gene            7069..8535
                     /locus_tag="DHV81_07120"
     CDS             7069..8535
                     /locus_tag="DHV81_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HCI94225.1"
                     /translation="MKKKTQKNKSIKLNYIYNVSYQILTLITPLITAPYLSRVLKADG
                     IGAYSYTYSLVSYFIMFAALGTVNYGNREISYLQEDRAKRTKTFWEIELLSVISVIVC
                     LCAYMVFTLAFGHSPLKKHLLLIEAVYLISVASDISWLFQGLEEFGKIVGRNVIFKII
                     NIAFIFIAVRSESDLLIYVAGVCLLELFANISIWFYLPQYVDRPKLKELKPFSHLRAT
                     LTLFVPTIATTIYTALDKTMLNNITGSMTENGYYEQANKISKMVLMLVTSLGTVMLPR
                     IGRAFSENKKDEVKALLYKSFQFVWFIGLALCFGLIGIARNFSPWFFGPGYEKVPYLI
                     MIMATLLPIIGLSNVIGIQYLVTTKREGLLTRSVCIGAVANFVMNMILIPQLYSYGAA
                     IASVISEVIITAVQLYFIRNELSIPKIFSLSWKYLVSAVCMLIVLLLMDAKLSVSFMH
                     TMIMIVTGFIIYMVLLLLMRDEMVCEGIRLIKARIKYH"
     gene            complement(8577..9692)
                     /locus_tag="DHV81_07125"
     CDS             complement(8577..9692)
                     /locus_tag="DHV81_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94226.1"
                     /translation="MKFIKIPAFAIVLLLLMQIVSKAFIAIDSSDIENIYGFYSEPKN
                     SLDMVMIGPSEVYTGYAPALAWEEYGLKSCNLSIGAVPCNMYKSIVTEIVKRQKPKLL
                     LINASAFSYCNSNLTDEVYLRKWIDNMPMSQNKLDTVKTIPKNINNDDEKVKDNISDT
                     LYFPLEKYHGNWKAPEAVYTSFVTRAYMRLSGGGYLKGFYSKTGITGGRDNLANIGQP
                     TKEAYVLTDECRAYLKELLSYCNKLGIKHVLLLQPPHETQAADKSGLEQIESITREYG
                     YDFLDLSTDYESIAGIDDSHDFADFEHLNAYGAQKLSSYIGNLVVNQYGIKSDTTGSE
                     LSRWEKSVKRTKKALKMAREYTDRREAQVVGEYEAAK"
     gene            complement(9797..10840)
                     /locus_tag="DHV81_07130"
     CDS             complement(9797..10840)
                     /locus_tag="DHV81_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCI94227.1"
                     /translation="MQTNTTRDKTIDFIKGILILFVILGHTTLGLTDMYSFDDTMNGI
                     ANVIYSFHMPCFIAVSGFLYSEYVGNASQGIFSRIGHKAFNILLPYVMISVIYWIIKF
                     ALSNLIREPVAVSSLISIPWKPIEFLWYLYALFLLYCVAYMVDYVFARLLPHFNYKME
                     LLFVLFLIIAFTCYGSFHYEGFNYIAGFQMYFYLGCLIKKWLDKLDNRYAVLSLAVMS
                     VLVEASGIVLQDDMSSNPLFSSLFERFTACIVTVFIFAVSYFLSRYCDFPKWLQTIGR
                     DSMPFYAMNVIFVGGIRIILNRAGITNMALQCICGFAGSTAICWILYECIIKKIRLID
                     FIFYPGKQLDVRK"
     gene            complement(10921..12051)
                     /locus_tag="DHV81_07135"
     CDS             complement(10921..12051)
                     /locus_tag="DHV81_07135"
                     /inference="COORDINATES: protein motif:HMM:PF03734.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94228.1"
                     /translation="MELETKGAVIFMKKKIIVLLTIAILSLCNTSVFAEESGYIWTQG
                     ESGLWYYVDTEGNCKTGWQYINGCWYYMDVNGIMTTGWQYINNHWYYMDANGAMTMGW
                     QYINGYWYYMDANGAMTTGWQYINGQWYYMDVNGIMTTGWQYIGGQWYYIAPSGIMVT
                     GWQYIGGQWYYIASSGVMTTGWQYINGNWYLMNGNGAMTTGWQYISNFWYYMNGNGVM
                     QTGWQSINGTWYYFNASGDWVDFSSMNSYAQGYSSSTKYLIMVDRKACVVGIYYGSRG
                     NWTQQYAWPCAPGKASTPTVGGEFNVGGKGYYFDSGNSRCYYYTQFKGNYLFHSVLYN
                     KNGTLQDGRVGIPLSHGCVRLEIGNAKWIYDNIPRGTHVVVY"
     gene            complement(12042..13595)
                     /locus_tag="DHV81_07140"
     CDS             complement(12042..13595)
                     /locus_tag="DHV81_07140"
                     /inference="COORDINATES: protein motif:HMM:PF01473.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HCI94229.1"
                     /translation="MRKKATNKTKKILGAFAIAGMLAMSVNSVQAAQEEYTAGSSMGK
                     EYDELEQAYSDDEVLATASNQGHWEVNYAGWWYQYSDGTYPRSSWVNISNKWYYFDEN
                     GYMVTGSRNINNAWYYFDGSGEMLTGWQYYLGAWWYHKPSGAWVQSNYANGTLVGIDV
                     SYYQKDIDWTTVKNSGINYAMLRVSASYYSDSYHHFTDKRFNEYASNANKAGMPIGAY
                     IYSKARNVSDAVSDARYVVSELKGYTISYPVAIDLEDSSQTDLSKAQLGAIAKAFCDE
                     IRRYGYTPMVYCNENWYKNYIDVSQIAGEELWIARYNSHYDTNIKRGIWQCSSTTRIP
                     GISGNVDLDFAYKNYENPITSVIGYWSLYGNDWYFIDANGQYVTGWQFINGNWYYFAG
                     NTVMTTGWQYVNGNWYYMDASGAMKTGWQYINGKWYFLEKSGTMTMGWQYISGHWYYM
                     DWTGMMTTGWQYIGGHWYYMDWTGIMTTGWQYIGGHWYYMNADGIMVTGRHYINKRWY
                     YFNANGVWN"
     gene            complement(13797..14864)
                     /locus_tag="DHV81_07145"
     CDS             complement(13797..14864)
                     /locus_tag="DHV81_07145"
                     /inference="COORDINATES: protein motif:HMM:PF01473.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94230.1"
                     /translation="MVRGWQKINSSWYYFDANGYMQKDWQEIGGKWYYFSPYDGFMYR
                     SRVSEINGTMRAFAPSGQMIQGWTWDVYTDNDGDVTGDWFYANPNGTAYEDGWLAQDG
                     GWYYINTYSGRMYSKGMYTINDKRYRFKKDGKLIIGWYQTGIYLDDWYYTNADGSAYS
                     GWLPYGGSWYYFDDWGDMYGDQWVDHKYYIGCDGRMVSGWYDCSQKSANYSSTSWMYF
                     NADGTKYVGWLPYNGNWYYISDNGYMVADTIDYCVSQDAFRDENGNTNWTEYNKARKA
                     SPRYVFDANGVMVTGWYHDIQITGGQITYDEWYYSDSDGKCHDGWLLYNGAWYYFDKG
                     EMLRNTYTPDGNWVGLDGVWA"
     assembly_gap    15251..15306
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15610..16995)
                     /locus_tag="DHV81_07150"
     CDS             complement(15610..16995)
                     /locus_tag="DHV81_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl glycosylphosphotransferase"
                     /protein_id="HCI94231.1"
                     /translation="MKKYFKKTIQSIIFILKVLLYLSLLFSFLILFSRNNFAVLNISR
                     TMATTILTYVFVGLLMTAIYGRYDIGRRKSKPIIYSLVLATLFTDFVAYLQLMIMNTI
                     TPNVSAFKFSDIGTLALVIVVHVVIIIVFVHIGNGIFFAINDPESCCVITTQESDLSK
                     LSSSVKRYKKQYCIKRVVDYRRKDLYDIIKDMDTVFIYDIPSDKKPDIINFCYERMIN
                     IYINPDVCDLVENVSEHYLLDDVSFLNHNVSNMTFEQRVIKRIFDIIFSLIFVVISSP
                     IWIVSSIAIKLGDGGTVFFKQKRATQYGRVFYVYKFRTMKEHVENRSATSDDDRITKV
                     GKVLRKTRLDEVPQLFNILKGDMSFVGPRPEMLENVKSYTRELPEFKYRLRAKAGLTG
                     YAQIAGKYNTSPKDKLILDMMYIENYSFRTDIKLLFQTAIVLLKKDSTEGFQKVSDCL
                     ECEKTPENLIN"
     gene            complement(17006..19459)
                     /locus_tag="DHV81_07155"
     CDS             complement(17006..19459)
                     /locus_tag="DHV81_07155"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13425.4,HMM:PF13692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94232.1"
                     /translation="MKSSKTIKIVMEMITYISLVLYMFDVVLLGTGELTKSFGIQSRM
                     IFFGISVLAAVVLILLDLKKYLNNKYLISVLVFMVVIFIAAIRGFVGKQNTTILLSDF
                     KGFLNFLIVFPMMAVMYKKNRVISFLKMLCLSLGVVSVLGIILAFYLQMPLEIRHWAY
                     DFFDGYNICMITELTGRVTRIFFNSGSRLFFAGFALSIVFSLIENKRKIIWIFIASLD
                     IFGVFISYTRSGYLAFAIGIFAFVVLILLFYRDFFKPLAIRACFVAGIVVVLIGTVSV
                     AEKANLVDVAINRCLFAGAEIEDNDTNVDKPSDNKNDKNNKNDKLTNEKAEIQNLAIR
                     EQRKTLALENIKKHPLFGGGLGVSNDINDGFIEYFYLDILSKIGFIGFIIFIIPFLFS
                     VFDTIILRDVFCKEQRLLALAAQLGCLFVFVISYFNPCLNSSVGLFMYSLALVLSMPW
                     ENKKPKTAYFISSTNHYNVRTGKFVNDYVKKGYDVIYVTSDFDHMTKKRYCFNEYKNS
                     KQLHVISYKKNLSISRILSHLMFSYKTFYMLLACKPELVYVEVPNNSLVKSSAKYKKI
                     NNAEIIVDVFDMWPESMPVKTKNMIVNRGFNIWRNFRNKNLKFADQIWIECDYYRELL
                     SAQKINLPMETKYLTLENAETSIETKVSEDEIDLCYLGSINNIIDISLIEKIVSELAK
                     NKRTRIHIIGDGERKDEFLEILKQNSIEIIDHGKVYEIDKLQEIFNQCWFGVNVLREG
                     LAIGITMKSISYFRGGLPIINTVQGDTSRFVEECNIGINVDRHDVKSCVSRILNITKD
                     DLACMKNNTRNLFEQKFAE"
     gene            complement(19446..20288)
                     /locus_tag="DHV81_07160"
     CDS             complement(19446..20288)
                     /locus_tag="DHV81_07160"
                     /inference="COORDINATES: protein motif:HMM:PF00535.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI94233.1"
                     /translation="MILAGITLFNPDVARLEENISSIYGQVDRVICVDNGSDNIKNIE
                     DCVLNNWKNITIIKNGKNEGIAKALNQMFEFAIEQQYEYVLTLDQDSVCPDNIIEEYN
                     KYLDEHKLGSLCPQCVDRNFKSELNNEDVIEVDKCITSASLVPVSAWNDVGGFNEELF
                     IDFVDHDFCAKLKEHGYRILQIGSVKLSHELGKGKNYSILGVKFTALNHSAMRKYYMV
                     RNWIYYMNEHKDIINLKAEKKSYRLFFFKTLIFEDDKLKKMKQMLKGRRDGIELIRRI
                     HEIK"
     gene            complement(20285..20992)
                     /locus_tag="DHV81_07165"
     CDS             complement(20285..20992)
                     /locus_tag="DHV81_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI94234.1"
                     /translation="MISVCVAAFNGEKYIEEQILSVLRNLDSQDEIIISDDGSSDRTR
                     RIVDDLAEKDRRIRIIDGPRKGLIKNFENAIVHSKGDIIFLCDQDDVWKDNKVKTVLD
                     IFGNTDCTLVMHDACIVDANLRISGCSFFEFKKCKKGYWRNLIKNSYIGCCMAFKRSI
                     LDYAIPFPDNIPMHDQWIGLLSERVGKVEFCNEQLLLYRRHSNNASEMTHLPLGEMVK
                     NRAIMLKCINKRIREKK"
     gene            complement(20995..22170)
                     /locus_tag="DHV81_07170"
     CDS             complement(20995..22170)
                     /locus_tag="DHV81_07170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94235.1"
                     /translation="MLLIGNYKTFIEKYNKEVFSIVYIFLLIHFFWGTTMIQIHLPRC
                     YTWICLVVLLGYIIGKILVEPNRKYLIIMATIVAPFIISGLLSGYHELMYVGLLIAAA
                     KDIPIDRILKDYLMVCIPFLLITILGSQIGIVENLIYPSLYRGNRIAYGFLSPTDFCA
                     HVLFIILVLFSVIKKKNYWKMALISVLIAAFLYFRCDGRTTVASLLIFLTGIAICYII
                     KKWVGLNKRIEWLLVDICESFIVIASFVLGWLYQLNNKYIIGLNDVLSDRLKYIAQTM
                     STYNIKLWGQYIEEHGNGGSIIAKDNYFFIDNSFVRCMYEYGAIVFVLVIVLIFMAQK
                     KMLDNEKYIMCLAFFIVTIASFAEHHLLEIAYNPFIFSVFACMSNNDKVIMEEKRIC"
     gene            complement(22183..23004)
                     /locus_tag="DHV81_07175"
     CDS             complement(22183..23004)
                     /locus_tag="DHV81_07175"
                     /inference="COORDINATES: protein motif:HMM:PF04991.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HCI94236.1"
                     /translation="MNRELSLEEVHKETLNVLKKIDEICEHNDIKYSIMYGTLIGYMR
                     HNGFIPWDDDMDVCMLRPEYDKFVAYCNEHEEELYPFKLMSKYTADDYPFNLQRFCDT
                     RFKMVKTDGLSDAGMGLFIDIYPLDALGNVKNGAKKQIEKKKTFWMQCVGCAQSKNIM
                     GTNKSFVKRLLRFPVYLYSHVKGTKYFLDKFDVLTNSYSYENSKYVGDLIWDNCVTYY
                     EKEWFEDVEDIIFEGVKTKIPVQAKKVLEEEYGDYMTPPPEEERVPYHEYIIYRK"
     gene            complement(23093..24502)
                     /locus_tag="DHV81_07180"
     CDS             complement(23093..24502)
                     /locus_tag="DHV81_07180"
                     /inference="COORDINATES: protein motif:HMM:PF14667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94237.1"
                     /translation="MKLKQSKVFKAGIGYTIGNVMVKGINFLAIPIFSRLLTTEEMGL
                     YTVFAAYEAVLFVFIGMALHSSIRSAKYEFEGKIDDFTSSIMGIYWINLIVALFISIV
                     FNKILTKLLDLDLVTLVMLVIYSFGIAVVTLYNARISLDYEYKKYIKVSLASTIGNVG
                     LSLILIKTIFNSSRGFGRVLGITISTVLVTVYIIYDLYKRARPTFRKKYWKFGIKYSL
                     PIIPHGISQVLLAQFDRIMINKMIGKSEAGIYGLVGNIKLILAIISDSISEVWMTWFY
                     EKMKNKEIEAVRSRARQLCYFFLIIAIGVMSISPELVLIIGGQKYAVGKYVAIPMILD
                     AFVLFIYNVIVPSEYFKQKTSFIMWGTMSAAVINIITNYIFIQKYGFIAAAYTTLFAY
                     ICYMILHLVISYRLMRFSIIPVSCIALCLLSIVIIAIVDLIFIDNIFVRYLVCFAVVM
                     FIGIKVLPEFVKENRRKAE"
     gene            complement(24629..25237)
                     /gene="rfbC"
                     /locus_tag="DHV81_07185"
     CDS             complement(24629..25237)
                     /gene="rfbC"
                     /locus_tag="DHV81_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016396235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCI94238.1"
                     /translation="MGQIKVQKNVGGIEGLCIIEPTVHGDNRGYFMETYNSKDMQEAG
                     IDRVFVQDNQSCSTKGVLRGLHFQKEYPQAKLVRAVKGSVFDVAVDLRSDSKTYGKWF
                     GVELTEENKKQFLIPEGFAHGFLVLSDVAEFCYKVTDFWHLGDESGLAWNDPAIGIEW
                     PQLVGEYPGSADGSGYTLEDGTPINLSDKDQKWETLENTFKF"
BASE COUNT         7787 a         5792 c         3599 g         7968 t
ORIGIN      
        1 cggatagcaa gcacctgata atcaccaaat ttctttggct cagcggcacg tgcctttgtc
       61 ataatctcat ttatctgtgc agcaccatca atgcccttta aggtcattgt ggaaatgccc
      121 tctctgtagt agccatactt ctcgtacatg tcaatcatgc catcccagag agtcttaccc
      181 tgtgacttgt agaatgcagc tacctcacac aacatacaaa ctgctgcagg agcatccttg
      241 tcccttgcat atgtgcccgg cagacatccg taactctcct ccataccgaa tacataattg
      301 ttgcagccgg ttttttcaaa tttgagcatc tgctctccga tatacttgaa acctgttaaa
      361 acctcgatat gctttacgcc atatgctgtc gcaattgcct tacccatatc tgtagaaaca
      421 attgactcaa caaatgcagg attctcaggc agagtaccat tggcactctt ctggcttaaa
      481 atatactcac cgataagcat agctgacata tttcctgtaa atgtcacata ctcacctgtc
      541 tttgtatcct tgcagtatac accgagtctg tcggcatcag ggtcagttgc taaaacgata
      601 tcagcatcct tttcctttgc aagctcaagt gcaagcttcc atgcatcagg attctcagga
      661 tttggataag gacatgtagg gaaattgcca tcaggcactg cctggcttgg ctcaatgtat
      721 acattctcaa agcccatatc cttaagtact ctctgtacag ggcgaagacc tgtgccgtgg
      781 agtggtgtgt aaacgatctt gatatccttt gccatagcct tgataatctc cggatggata
      841 gaaagcttgc ggagctctcc gaaatatccc tcctcaacct catcagagat gacattgtaa
      901 agaccagcct caatagcagc agctttgtcc atagtcttta cctggtcaaa tgatgtaaca
      961 tttgccacct cagccatgat gcttgtatca tgaggtggag taacctgtgc accatcctcc
     1021 cagtatacct tgtatccatt gtactcacga ggattgtggc ttgctgtgat aacaacaccg
     1081 gctattgtac cgagtgtcct tactgcgtat gacagctcag gaacaggtgc gagctgtggg
     1141 aatacatatg tcttgattcc gttagctgca agacagagtg ctgtggcatc tgtgaactca
     1201 tcatgcatat tacgtgagtc atgtgcgatt gcaacacctc tctttactgc gtcctctccc
     1261 tgtgaaagta tgtaattcgc caatccctgt gtagcctgac ggactgtgta tatattcatc
     1321 ctgttggtgc cggctcctat tacaccacga agacctgctg taccaaactc aagtgtgcga
     1381 tagaatctgt cctctatctc cttatcatca cccgcgatag ccttaagctc tgcctttgtg
     1441 gcctcatcaa aatatgggtc tgtacaccat ttctcgtact ccgctctaaa atccatttat
     1501 attaacctcc tgtgtcagct agtaataagc ctattaatgt taactatacc gattttacca
     1561 cattaacgtg catttgaaaa gttttagttc cttaatattt ttttaaacac caggctcctc
     1621 atccaaagtg gcatcataca gacaacaaac tgtgccgcaa ccgtaacaaa ataatccgcc
     1681 gcacttatgg ttcctgtctt taaaatacgc tttcttcctg ccttgtattt aaggaaataa
     1741 tcaaagccgc cacgcctctt cagcatatca tcaccggttc tggcatacac aagagtctca
     1801 cctatatttg ccatacgaca gtgatttctg atcatattcg cccaaagaag agaatcctcc
     1861 ataagcaggc agtcctgata attgcctgca gcaagcaccg cagacctttt gtacatcaca
     1921 gtcatgtgat tgaacgcatc gcgctttctc tgatactgct ttatcacata atttccgaga
     1981 ggcactgtcc tctccgataa aatattacgt gtatcatcgg aaaactcgct gatgtagccg
     2041 cctatgatat caagccccgg attttccgaa aaagcattca gctgtctctc aaaccggtca
     2101 ggcacacata tatcatccgt atccattctg gccacaagct cattagagca atgctttatg
     2161 ccttcagcaa gtgcaagacc gagcccactg tttttttcaa gctttaccgt aacaaaactt
     2221 atttccgaag gcctgcattc ataatctctg attgcctcat aaagctcctc tgtcagccgg
     2281 ccatcctcca ccagcactat ctcatccggt aaaacagtct gcgaatatat gctttccata
     2341 cactccctga aatactcagg cttttccttg taataaattg acagtaagac tgaaaatttg
     2401 actttattca taagtatcat cctcccagac aagtaaacta atgtattacc gcaagcacag
     2461 tctctataca cagtctgata tcatttatca gcgaaaatct cttaagatac ttaagattgt
     2521 acctcatctt gcccggcagc accttgtgga tatatgtgta atccacatca tctgcagcct
     2581 ccagaagcct gtcctcatcc ttatacctga tgcttgcctc acttgtgatt ccggcaggca
     2641 tcagaagtgt cgccatcatc gcaggtgtat atgccatgac atacttctcc acctcaggtc
     2701 ttgtgccaac aaagctcata tcaccacata gcacatttat aagctgcgga atctcatcaa
     2761 gcctgtactt tctgatgaca gcaccgactc ttgtgattct tgaatcgcca ccgacagtca
     2821 cgagtgaacc ctttttgtca gcatcattca ccatagttct aaacttgaat atgcgaaact
     2881 ttctgccata ctgggtgatg cgctcctgtc tgaaaaaaac aggtcctctg ctatctacct
     2941 ttatccagac agcaaggacc agtataacag gcgaaagcag cacaagaaga acagcggaca
     3001 taatcacatc aaaaatgcgt ttaaatacca tgcttgtcct ctttctgctc agatgcctgt
     3061 aatatttaag tgtaaaatca tttttcatga atgcaggaat ctctccccat gttctccatc
     3121 tactcatgta aaatctccaa taaattatta attatatact ccacatcctc atcgctaagc
     3181 tttgtgttaa gaggaagtgt aatctcattc tcatactgat gataagcatt tggaaaatca
     3241 gctatatcaa agcccatatt cttatatgct gtcatcatcg gaagcggctt gaaatgcaca
     3301 tttgtggcga tacctcgctc cgccatctgc actataagcg cattgcgcct gtccacgccg
     3361 atgcccggta tgcgaaacag atacagatgt ccgctcgatg tatgcctgtc tgtaaaatga
     3421 ggcacagtca agagaccaag cggcttaaat gcctcgtcat agcgctctat aatatcacgg
     3481 cgcctcttga gaagcccctc atatctgtca agctgtacca gaccaatagc cgcctggata
     3541 tcagtcatat tgcacttata cgaaggagcc acaatatcgt actcccagtt gcccagacca
     3601 ttcttgtgca aagcatcctt tgtctgtccg tggagtgaaa gcagcataaa ctgcttgtag
     3661 atatactcgt catcaaagcc ctctataggt ctccaggtga gtgctcctcc ctctgcggtg
     3721 gtcagattct tgaccgcatg gaatgaaaag cttgtaaaat cagctacatt gccacacata
     3781 atacactcac ctgctctcat gtaagaagca ccaaaagcat gagctgagtc agccgccaca
     3841 atcacacgtc cgattgccct ctgcacatca tttgacggat gaaaaagagc cttctttctc
     3901 tccacaatat caaaaatcct gtcatagtcg caaggcacac ctgcaatatc aaccggcacg
     3961 ataacctttg tcttttctgt aatagcagct tccagggcat cgtaatccat ctcaaatgaa
     4021 tcaggtgcta catctataaa cttaagtgtc gcacctacat gtacagccgg gcttgcagat
     4081 gcagtatatg tgtatgcact tgtgatcacc tcatcacctg cacctattcc caggaatctg
     4141 agtatcgact caagacacgc agtagcagag ccaagagcta cagccttatc cgtgccgcat
     4201 agcttagcaa tccgcttctc aaactccttg gttcttggac ctgttgtaat ccaaccgctt
     4261 cgcagtgcat cagcaacagc tgacacctct gcctctgtta tatcaggtgg tgaaaatgaa
     4321 atctgtctct tttccatagt tatgttcctc gcttccggca taaaatatgc caaacatccg
     4381 gcttttacag tctgtattat gagcttcaaa agatatttag ttctccaggt caggatgata
     4441 tgtctttacc acagaggcca ctatctgctt gatatccttt gcttctgagg tgcatgcctt
     4501 ctcgagcatc tcaagctgct tctcaaagag ctcatcatcc atctcaatag gctcacccac
     4561 atgaatcagc ttgttcggag tctccttgag accctcctcg ctcatcagca tctcctcgta
     4621 gagcttctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnaaaatctc gcctccgtga gcatagagcc ctgcctgcag cacaagcgcc
     4801 acagcctccg gaattgtcat aaaatacctg ataatacgct tgtccgtcac agtaacagga
     4861 ccgccatcct ctatctgctt cttaaacagc gggataacac taccattgct gccaagcacg
     4921 tttccaaaac gcacagcgac aaaatcagtc tcagtctgcc tgttcatcat ctgtataacc
     4981 atctcacaca gacgctttga tgcacccatg atattggttg gattaaccgc cttatccgta
     5041 gagataagca cgaacttctt cacatggtac tctgctgcaa tccttgagag ctttaatgtg
     5101 ccaagcacat tgttctttat tgcctcattc gggctttcct ccatcagcgg tacatgctta
     5161 tgtgctgccg catgataaat aatctccgga tgatatgtct ggaatacata gtgcagcctg
     5221 ttagtattgc gcaccgagcc aatcagcgtc tccaccctta cataaggata tttgcgcttc
     5281 agctcctgct gtatatcgta tgcattattc tcgtaaatat caaaaatgat aagaagcttc
     5341 ggatcctccc tggctatctg acggcaaagc tcactaccga ttgagccacc gccacctgta
     5401 accatgacca ccttgccctg taaaaatgaa tgaataccgt cattatccac aacaaccgga
     5461 tctctgccca gcaaatcctc aatgcttacc ggacgtagat tggaaaccga cactcttccg
     5521 tccaccatct gatagattcc cggaaccaca gacacatcac agcctgtgtc cttacaaata
     5581 ttgagtatat ccttcctgtc cttaccggat gtagaaggta cagcatacac aattttcgtg
     5641 atatcatact tctcaaccat ctccggaatg tcatttcggt tgccgacaat aggcacgccg
     5701 tctataaagc gccccagctt cgccggattg tcatctatga tgcagcaggc ctttgtgtgc
     5761 agatgtgagc tcatgataag ctccctgata agcatcctgc ccgcatttcc ggctcctata
     5821 atcatgacct tctcatcacc gtttgcagcg cctctctgct caagcatctt ctgacctgtc
     5881 ctgaaaaatc tgtacgcaaa acgacacgct gtgcacagtg caaagctgag catcagtccg
     5941 ataatccaga aggcatacgg catcttaatc tcatacacct catgaatagt aacgctcaca
     6001 accgcaagca ccagaaaggc accaataata tgcataaatt cattgatgct cgcaaaacgc
     6061 catatactgt ggtaaagtct gaaaatataa aacaccacaa gtgaaatcac cataaacaat
     6121 ggcacaatgt gcagataacc ctccaaaaac gagagcggaa tatgtgaata tgaaaactca
     6181 aacctgatca tcaaagcgaa aaagaatgag attgcaataa tacacatatc aacaaaagca
     6241 agaaccaatg ccctgaaaac ccattgcttg tcattgccca tgtatgcgtc cggcttctcc
     6301 ggaccatcgt attttgtttt gtcttttcga ttatttgtat cttttttcat atcaagtcta
     6361 ttactcatac aggattttct ccttctgtcc cacactgcaa aaagcagctt ctatatccct
     6421 atgtaaatta acttctcata aactgcattt aaatttacgt tttattatac catacataca
     6481 ctattatagt tcttattatt ttattaaata taccacattt agcgattttt gaaaacaaac
     6541 agcttgctta tcacatagtt gagcaccacc acaaccacct gcgctgccag cttcacccac
     6601 atgctgggat agtccagaag cgtgataaaa ataaagataa tagtctcttc aataacaagc
     6661 gtgatgaatc tgccactgaa aaaagcaaac atctgtacta aaaatgccgt cttggtgggc
     6721 gttgcagcct taaacaccca cttcttgttg gtaacatatg aaaaaagaac agcaaacacc
     6781 catgccagtg cgttcgcaat cagctcattg ataccaaaga atacattaaa aatggcatat
     6841 gatcctatgc tgatcacaaa ggtcatattg ccaaaaaacg tgtataaaac aagctcctta
     6901 aactcctctt tcctgtttcg tattataata ttatccattt ctccacctat gtgacaggct
     6961 ttagcccctg tcgtaattta tacgtgtaag ttgatagtaa tgttaatttg cgatttttat
     7021 ataataactg catagccaaa ataagtcaat atgtgatatg atatctgcat gaaaaagaaa
     7081 acacaaaaaa acaagagtat aaaactaaac tacatatata atgtgagcta ccagatactc
     7141 acactgatta caccgcttat cacagcgccg tacctctcga gggtgcttaa ggcagacgga
     7201 ataggtgcat acagctatac ctactcactg gtatcatact ttatcatgtt tgccgcactg
     7261 ggcactgtaa actacggaaa cagggaaata tcatacctgc aggaggacag agcgaaacgc
     7321 acaaaaacct tctgggagat agagcttcta tccgtcatca gtgtgattgt gtgtctttgc
     7381 gcatatatgg tattcacact tgcatttggg cactcaccac tcaaaaagca cctgctattg
     7441 attgaagccg tgtacctgat atcagttgct tctgatatct cctggctgtt tcagggactt
     7501 gaggaatttg gcaaaatagt cggtcgaaac gtgattttta aaatcataaa cattgctttt
     7561 atcttcatcg ccgtgcgctc agagagtgat ttgctcatat atgtggcagg agtatgtcta
     7621 ttagagcttt ttgccaatat atccatctgg ttttacctgc ctcaatatgt tgacagacca
     7681 aagcttaagg agcttaagcc attctctcat ctaagggcta ctctcacact atttgtacca
     7741 actatagcga caaccatata cacagcgctt gacaaaacca tgctcaacaa cataaccggc
     7801 agcatgacag aaaacggcta ctatgagcag gccaacaaga tatccaaaat ggtgctcatg
     7861 ctcgtaacat cactcggtac tgtgatgctt ccaagaatag gaagagcctt cagtgaaaat
     7921 aaaaaagacg aagtaaaagc cctcctttat aagagctttc aattcgtctg gtttataggt
     7981 cttgccctgt gctttggcct gataggcatc gcgcgcaatt tctcgccatg gtttttcggg
     8041 cctggatatg aaaaggtgcc atacctcatc atgataatgg ctacactgtt gccgattata
     8101 ggtttaagca atgtaatcgg tatacagtat ctggtaacta ccaagcgtga aggtctgctg
     8161 acacgctcag tatgtatcgg agccgtagcc aattttgtca tgaatatgat tctcatacct
     8221 cagctctatt catacggtgc agccatagca tctgtcatct cggaggtaat aataactgcc
     8281 gtgcagcttt acttcatacg aaatgagcta agtataccaa aaatattttc tttatcgtgg
     8341 aaatatcttg tatcagcagt ctgtatgctt atcgtactgc tgctcatgga tgcaaagctg
     8401 tcagtcagct tcatgcacac aatgattatg attgtgaccg gatttataat ctatatggtg
     8461 ctgcttttac tgatgcgtga cgagatggta tgtgaaggca taaggctcat caaagccaga
     8521 atcaaatacc actaaataat accattctgc aacgcttaat tccccggcag cttcacttat
     8581 ttggctgcct catactctcc aaccacctgt gcctctcttc tgtcagtata ttctcttgcc
     8641 attttcagag cttttttagt cctcttcacg gacttttccc atcttgaaag ctctgaacct
     8701 gtcgtatcag actttatccc atattggttt actaccagat tgccaatata tgacgaaagc
     8761 ttttgggctc catatgcatt cagatgctca aaatctgcga aatcatgtga atcatctatc
     8821 cctgcaatgc tctcataatc agtactcaga tcaagaaaat cgtatccata ctccctcgtt
     8881 atactttcaa tctgctcaag ccctgactta tcagcagcct gtgtctcatg aggtggctgc
     8941 agcaacagca catgctttat gccaagctta ttgcagtacg aaagcagctc cttaagatat
     9001 gctctgcatt catcagtcag cacataggct tctttagtag gctgtccgat atttgccaga
     9061 ttgtcacggc ctccggtgat gcctgttttt gaataaaagc ccttcagata gcctccacca
     9121 ctgagcctca tataggcacg agtgacaaat gaggtataca ctgcctcagg ggccttccag
     9181 ttgccatgat acttctccaa tggaaaataa agggtatccg atatattatc cttcaccttt
     9241 tcatcatcat tattgatatt tttgggaata gtctttaccg tgtcaagctt gttctgtgac
     9301 ataggcatat tatcaatcca ctttctcaga taaacctcat cagtcagatt actattgcaa
     9361 taactaaatg cagaagcatt gattaaaaga agcttcggct tctgtctctt cacaatctcc
     9421 gtgacgatgg acttgtacat attgcatggc acagctccta ttgaaagatt gcacgactta
     9481 aggccatact cctcccacgc caacgccggt gcatagcccg tgtatacctc acttggtcca
     9541 atcatgacca tatcaagact gttctttggt tctgagtaaa agccatatat attttcgata
     9601 tcgctgctgt caatggcaat aaatgccttt gatactattt gcatgagcaa aaggaggact
     9661 attgcaaatg caggaatttt aataaatttc atggtgtttc cttttctatt gaaacgcttt
     9721 attttgatat tttttcagta aacactatat agtatatcgg attagtatta aaaaacaaca
     9781 ccctgatttc tatttgtcat tttctgacat cgagctgttt acccgggtag aatataaaat
     9841 cgataagcct gattttcttt atgatacact catacaatat ccagcatata gcagtgcttc
     9901 ctgcaaatcc acatatacac tgcagcgcca tattggttat accagccctg ttcaatataa
     9961 tcctgattcc acctacaaat atcacattca tcgcataaaa cggcatgcta tcccggccga
    10021 tagtctgtaa ccatttaggg aaatcacaat accttgataa aaaatagctt actgcaaata
    10081 taaatactgt cactatacag gcggtaaacc gctcaaacag actgctgaaa aggggattgc
    10141 tgctcatgtc atcctgtaat actatgccgg acgcctctac aagcacactc ataacggcaa
    10201 ggctcagcac agcatatcta ttatccagtt tatcaagcca cttcttaatc aggcatccca
    10261 gataaaagta catctgaaag ccggctatgt agttaaagcc ctcatagtga aagctgccat
    10321 agcatgtgaa agcaattatt aaaaacagca caaataaaag ctccatttta taattaaaat
    10381 gtggcagaag cctcgcaaac acataatcaa ccatatacgc aacacaatat aagagaaaca
    10441 gtgcgtataa ataccataaa aactctatcg gcttccacgg tatgcttatc aggctgctaa
    10501 cggcaaccgg ctcccttatc aaatttgaaa gggcaaattt aataatccag taaataactg
    10561 aaatcatcac gtacggcagc aggatattaa atgctttatg acctatccgg ctaaaaatgc
    10621 cttgtgaagc attccctaca tattcactgt ataagaagcc tgaaactgcg ataaagcaag
    10681 gcatgtgaaa gctgtatatt acatttgcaa taccattcat tgtatcatca aatgagtaca
    10741 tgtctgtcag acccaatgta gtgtgaccta aaatcacaaa taaaataagg atgcccttta
    10801 tgaaatcaat tgttttatct cgtgtggtgt tggtttgcat gttatgccct caaatttata
    10861 aaaataaata taattcatca tttttatttt gtctaataga aattttaata aatattctct
    10921 ttaataaaca actacatgtg ttcccctcgg aatgttatca tatatccatt tagcgttccc
    10981 tatttcaagc cttacacatc catgagacag cggaattccg actctgccat cctgaagtgt
    11041 accatttttg ttatacaaca cactatggaa cagataattt cccttaaact gtgtataata
    11101 ataacatctt gaattgccgg aatcaaaata gtatccctta ccgccaacat taaattcgcc
    11161 tccaacagtc ggagttgatg cttttccagg tgcacacggc catgcatatt gctgagtcca
    11221 attacctcta cttccgtaat aaatgcctac aacacaagct tttctgtcca ccattattaa
    11281 atattttgtt gaacttgaat atccctgtgc atacgaattc atgcttgaaa aatcaaccca
    11341 atctccactc gcattaaaat aataccatgt gccattaata gattgccaac cggtctgcat
    11401 aaccccattt ccattcatat aataccaaaa attagatata tactgccagc ctgttgtcat
    11461 cgctccattt ccattcatga gataccagtt gccatttata tactgccaac ccgttgtcat
    11521 cactccactt gaagctatat aataccactg accacctata tactgccagc ctgtaaccat
    11581 aattccactt ggagctatat aataccactg accacctata tactgccagc ccgttgtcat
    11641 aataccattg acatccatat aataccactg accatttatg tactgccagc ctgttgtcat
    11701 agccccatta gcatccatgt aataccagta gccatttata tattgccagc ccatggtcat
    11761 agccccatta gcatccatat aataccagtg attatttatg tactgccaac ctgttgtcat
    11821 aataccattg acatccatat agtaccaaca tccatttatg tactgccaac ctgtcttaca
    11881 attaccttct gtatccacat aataccacaa accactttca ccttgtgtcc atatgtaacc
    11941 tgactcttca gcgaaaacac tggtattaca caagctcaga attgcaatcg ttaataaaac
    12001 tattattttt ttcttcataa atataacagc tcccttcgtc tctaattcca tacaccattt
    12061 gcattaaaat aataccatct tttattaata taatgtctgc cagttaccat tattccatct
    12121 gcattcatat aataccaatg tccgcctata tattgccaac ctgttgtcat tattccggtc
    12181 cagtccatat agtaccaatg tccacctata tactgccaac ctgttgtcat cattccggtc
    12241 cagtccatat aataccagtg tccactaata tactgccagc ccatagtcat ggttccactc
    12301 ttttccaaga aataccattt accatttatg tactgccaac ctgtcttcat agctccactt
    12361 gcatccatgt agtaccaatt accgtttaca tattgccagc ctgttgtcat aactgtattt
    12421 cccgcaaaat aataccagtt tccgtttata aactgccagc ctgttacata ctgaccattt
    12481 gcatcaataa aataccaatc attaccatat aggctccaat atcctatgac gcttgtaatc
    12541 ggattctcat aattcttgta tgcaaaatcc aagtccacat ttcccgatat acccggaatt
    12601 ctggttgtag agctacactg ccatatacct cttttaatat ttgtatcata atgcgaatta
    12661 tatcttgcaa tccacaattc ctctcctgca atctggctta catctatgta gttcttatac
    12721 cagttctcat tgcaataaac cataggagtg taaccatatc tcctgatttc atcacaaaat
    12781 gcttttgcaa tagcaccaag ctgcgcctta gacaaatctg tctgcgaaga atcctccaaa
    12841 tcaatagcta caggatatga gattgtatat ccctttaact cacttacaac gtatcttgca
    12901 tccgaaacag catcactaac gtttctcgct ttactatata tatatgcacc aataggcata
    12961 ccagctttat ttgcatttga agcgtattca ttaaatcttt tatctgtaaa atgatgatat
    13021 gaatcagaat agtagcttgc tgaaactctc agcattgcat agtttatacc actatttttc
    13081 acagtagtcc agtcaatatc tttttgataa tatgatacat caattccgac taatgtacca
    13141 ttagcatagt tagactgtac ccatgccccg gatggcttat ggtaccacca agcccctaaa
    13201 taatactgcc agcctgtaag catctctcca cttccatcaa aataatacca ggcattatta
    13261 atattgcgtg atccggtaac catatatcca ttctcatcaa aataatacca tttattgcta
    13321 atatttaccc aggaactacg tggatatgtt ccatctgaat actgatacca ccagcctgca
    13381 taattcactt cccaatgccc ctgattggat gctgttgcta agacctcatc atctgaatat
    13441 gcctgttcca gttcatcata ttcctttccc attgatgatc cggctgtata ttcctcctgt
    13501 gctgcctgta cagaattaac actcattgca agcatacctg caatggcaaa tgctcccaaa
    13561 atcttcttag ttttatttgt tgcttttttt ctcactgttt tatttccttc ctatactttt
    13621 aaaacattat agcacatatg agcttagagt accatcttga ttcaaataat accccagtat
    13681 ccagtattgc actcaaataa atacaaaatt ctaatgatta tatattaaat gaggtctatg
    13741 ttatccacat agacctctca aaaatatttt atgcaattaa tatataaaac tatgagttaa
    13801 gcccaaacac catcaagccc tacccaattt ccatcaggag tatatgtatt tctcagcatt
    13861 tcgcctttat cgaaataata ccaagctccg ttgtataaaa gccatccgtc atgacacttt
    13921 ccatcagagt ctgaataata ccattcatcg tatgtaatct gaccacctgt aatttgaatg
    13981 tcatgatacc agcctgtaac cataacacca tttgcatcaa atacgtatcg tggtgaagcc
    14041 ttacgagcct tattatactc tgtccaatta gtatttccat tttcatctct aaatgcatcc
    14101 tgacttacac aataatcaat agtatctgct accatataac cattatcact aatataatac
    14161 caatttccat tatatggtaa ccagcctaca tatttagtac catcagcatt aaaatacatc
    14221 cagctagtcg atgaataatt tgctgatttt tgactacaat cataccagcc tgagaccatt
    14281 ctaccatcgc atccaatata gtatttatgg tctacccatt gatcgccata catatctccc
    14341 caatcatcaa aataatacca gcttccgcca tatggcagcc atcctgaata cgctgatcca
    14401 tctgcatttg tatagtacca gtcatctaaa tatattcctg tctgatacca tccaatgatg
    14461 agcttaccat cttttttaaa tctgtagcgt ttgtcattaa tagtatacat tccctttgaa
    14521 tacatcctgc cgctatatgt gttaatataa taccagccac catcttgtgc caaccagcca
    14581 tcttcatatg cagtaccatt tgggtttgca taaaaccaat ctcctgtaac atcaccatca
    14641 ttatcagtat atacatccca agtccagccc tgaatcatct gaccagaagg agcaaaagca
    14701 cgcattgtac cattaatctc acttactctg ctacgataca taaagccatc ataagggctg
    14761 aaataatacc atttgccacc gatttcctgc caatctttct gcatataacc attagcatca
    14821 aaataatacc atgaagaatt aattttctgc cagcctctta ccatatagcc attagcatca
    14881 aagtagaatt ttccggtctc tgtagaaaca aactgactat atgcccaaga gccatcacca
    14941 aatttatagc aataatcagg atatccatcc tctggtccat accatccagt ttttgctcca
    15001 taaactgaag tagcctgtgc aagctcctca tcggaatctg tctctgtagc aactactgcc
    15061 ggtgtctcac tctctgttgg agtctcaact tctgctggtg tatctacttc cgttgaagcc
    15121 tctgtctctg caggtgtctc tgtttctgca ggtgcctctg tctctgcagg tgcctctgtt
    15181 tctgcaagtg cctctgtctc tgcaggtgtc tctgtctctg caggtgtctc tgtctctgca
    15241 ggtgcctctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnngtct ctgcaggtgc ctctgtctca gaagttatag gctgaatctc tgctgcactt
    15361 gcaagtgatg gtgttaccat cgtcattgct gtaacaattg ctgctgtcat tatgagtttg
    15421 tttttcataa acacagtacc tcctttttta ttatatatat ataattttag tacttatgtt
    15481 gtgatattgc aagtaattta ttaaaaaatc cacaaaatgt tacaaaatat tttttcttct
    15541 ttagtaaata ttatatgggt aacacttgca acaatttctt acaagtatta catattttta
    15601 taaaatgtct taattaatta aattctctgg tgtcttctca cactctaagc aatctgacac
    15661 tttttggaat ccctcagtac tatccttctt gaggagtaca atagctgtct ggaacaataa
    15721 tttgatatct gttctaaaac tatagttttc aatatacatc atatcaagta taagcttatc
    15781 ctttggtgat gtattatatt ttccggcaat ctgagcatat ccagttaatc cagcctttgc
    15841 cctcagtcta tatttaaact ccggcagttc tcttgtatag cttttcacat tttcaagcat
    15901 ctctggtctt ggtccaacaa agctcatatc tcccttcaat atattgaata gctgaggcac
    15961 ctcatcaagt cttgtctttc taagtacctt tcccaccttt gttattctgt catcatccga
    16021 tgttgctgat cggttttcaa cgtgttcttt cattgttcta aatttataaa cataaaacac
    16081 tcttccatat tgtgttgcac gtttctgttt aaaaaatacg gttccaccat ctcctaattt
    16141 tattgcaata gaacttacaa tccagattgg tgaactaatc accacaaaaa tcaaagagaa
    16201 tataatatca aatattcgtt ttatcactct ctgttcaaaa gtcatattgc tgacattgtg
    16261 attaagaaac gatacgtcat ccagtaaata atgttctgat acattttcaa ccaaatcgca
    16321 tacatcagga tttatatata tatttatcat tcgctcataa caaaaattaa ttatatcagg
    16381 ttttttatct gaaggaatgt catatataaa cactgtatcc atatctttta tgatatcata
    16441 aagatctttt cttctataat ccactaccct tttaatgcaa tactgctttt tgtaacgttt
    16501 tacagaagaa gaaagctttg acaggtctga ctcctgtgta gtaatcacac aacagctctc
    16561 aggatcattt attgcgaaaa atatgccatt accaatatga acaaaaacaa taataataac
    16621 aacatggact acaattacaa gtgccaaagt acctatatca gaaaacttga atgcggaaac
    16681 atttggtgtt attgtgttca ttatcattaa ctggagatat gcaacaaaat ctgtaaacaa
    16741 tgttgctaat accaacgaat aaattatcgg cttactcttt cttctaccaa tatcatatct
    16801 gccatatatc gctgtcatta agagtccaac aaatacatac gtaagaattg tagttgccat
    16861 ggttcttgaa atatttaaaa ctgcaaagtt atttctcgaa aacagaatga gaaaactgaa
    16921 cagtaatgat aaatatagca agactttcaa tataaaaata atagattgaa ttgttttttt
    16981 aaaatatttt ttcatgtttt ttatcctatt ctgcaaattt ttgttcaaac aagtttctgg
    17041 tattattctt catacaagct aaatcatctt ttgttatatt aagaattctt gaaacacaag
    17101 atttgacatc atgtctgtca acatttatgc caatgttgca ttcctctaca aatctacttg
    17161 tatcaccctg aacagtgtta ataatcggaa gtccgcccct aaaatacgaa atagacttca
    17221 ttgtaattcc aatcgctaaa ccttctcgta agacattaac tccaaaccaa cactgattaa
    17281 aaatctcttg caatttatct atctcatata cttttccgtg atcaatgatt tctatactat
    17341 tttgtttcag aatctcaagg aattcatctt ttctttcacc atcaccaatt atatggattc
    17401 ttgttctttt atttttcgca agctctgaaa caattttttc aattaatgat atatcaatga
    17461 tattgtttat actacctaaa tagcataaat caatttcatc ttcggatact ttcgtttcta
    17521 tacttgtttc tgcattttcc aatgtcaaat atttggtttc cattggaaga ttaatctttt
    17581 gagccgacaa taactctcta taataatcac actcaatcca tatttgatct gcaaatttta
    17641 aattcttatt tctgaaattt ctccaaatat tgaatcccct gtttacaatc atattctttg
    17701 tcttaaccgg catagattcc ggccacatat caaaaacgtc cacaataatc tcagcattat
    17761 taatcttttt atattttgcc gaacttttta ctaatgaatt atttggcact tctacataca
    17821 ccaattctgg tttacaagcc aataacatgt aaaacgtttt gtaactgaac atcagatgac
    17881 tcaatatcct gcttattgat aaattttttt tgtaagagat tacatgcagt tgcttactat
    17941 ttttatactc attgaaacaa tacctctttt tggtcatatg atcaaaatca gaggttacat
    18001 aaattacatc ataacctttt ttaacataat cattaacaaa ctttcctgtt ctcacattat
    18061 aatggttagt agaacttata aaataagcag tctttggttt cttattttcc cacggcatag
    18121 ataaaacaag agccaatgaa tacataaata atccaacact tgagtttaag caaggattaa
    18181 aatatgatat cacaaaaaca aacaaacagc ctaactgtgc tgccaaagct aataatcttt
    18241 gttctttgca aaatacatca cgtaaaataa tagtgtcaaa aacagaaaat aaaaaaggaa
    18301 taataaaaat tataaagcct ataaaaccta ttttacttaa aatatctaaa taaaaatact
    18361 caataaaacc atcatttata tcattggata ctcctaatcc tccgccaaat aatggatgtt
    18421 tctttatatt ttctaatgct aacgtctttc tttgctctct aatggctaaa ttttgaattt
    18481 cagctttttc gtttgttaat ttgtcatttt tattattttt atcattttta ttatcagaag
    18541 gtttgtcaac atttgtatca ttatcttcta tttctgctcc ggcaaataaa catctattta
    18601 tggccacatc aaccaaattt gctttttcag cgacactaac cgtaccaatt aacacaacaa
    18661 ctattccagc aacaaaacat gcccttatag ccaatggttt aaaaaagtct ctataaaata
    18721 ataatattaa tactacaaat gcaaatattc caatagcaaa tgccaagtag cctgatcgtg
    18781 tatacgaaat aaacacacca aaaatatcta acgatgcaat aaatatccaa ataattttac
    18841 gtttattttc tattaacgaa aatacaatgc ttaaagcaaa accagcaaaa aaaagtctac
    18901 ttccagaatt gaaaaaaatg cgagtcaccc ttcctgtcaa ttctgtaatc atgcatatat
    18961 tatatccatc aaaaaaatca tatgcccagt gtcttatctc taaaggcatc tgcagataaa
    19021 atgccagaat aatacctaac acagatacaa ctccaagtga caaacataac attttcaaaa
    19081 atgaaatcac tctatttttt ttatacataa ctgccatcat tggaaaaact atcaaaaaat
    19141 tcaaaaaacc tttgaaatca gacaaaagta ttgttgtgtt ttgttttcca acaaagcctc
    19201 taatagcagc tataaatata acaaccatga aaacaagtac acttattaaa tacttattat
    19261 tcaaatattt tttcaaatcc aataatataa ggacaactgc cgctaaaacc gatattccga
    19321 aaaaaatcat tctactttga attccaaagc tctttgtcag ttcccctgtt cctaataata
    19381 caacatcaaa catatacaaa actaaactaa tatatgttat catctccatt actattttga
    19441 ttgttttact tgatttcatg aattctcctt attaattcaa tgccgtctct tctacccttt
    19501 agcatttgtt tcattttttt caacttatca tcttcgaata ttagtgtttt aaaaaaaaat
    19561 aatctatatg atttcttttc tgcctttaga tttattatat ctttatgttc attcatataa
    19621 taaatccaat ttctaaccat ataatatttt ctcattgctg aatgattaag tgcagtaaat
    19681 ttaacgccta aaatactata attttttcct tttcccaatt catggctaag cttcacacta
    19741 ccaatctgaa gtattctata cccatgttcc ttaagcttag cacaaaaatc atgatcaaca
    19801 aagtctataa aaagctcttc attaaatccg cccacatcat tccaagctga aaccggcacc
    19861 aatgaagctg atgttatgca cttatccacc tctataacat cttcattatt caattcactt
    19921 ttgaaatttc tatcaacaca ctgtggacat agtgaaccca atttatgttc atctaaatac
    19981 ttattatatt cttcaattat attatccggg catactgagt cttgatctaa agtcaaaaca
    20041 tattcgtatt gttgttcaat ggcaaattca aacatctgat tcaaagcttt agcaatgcct
    20101 tcattttttc cattcttaat aattgttata tttttccagt tatttaatac acaatcctca
    20161 atgttcttta tattatccga accattatcc acacatatta ccctatcaac ttgtccataa
    20221 attgatgata tattctcttc tagtcttgca acgtctggat tgaataatgt tatacctgct
    20281 aatatcattt cttctccctg attcttttat ttatacattt cagcataatc gctctattct
    20341 taaccatctc acccaaaggt aaatgagtca tttctgatgc attatttgaa tgtcttctat
    20401 ataaaagaag ctgttcgtta cagaactcaa cttttccaac tctttccgac aacaatccaa
    20461 tccactgatc atgcattgga atattatccg gaaaaggaat tgcataatcc agtatgcttc
    20521 gtttaaaagc catacagcaa cctatatatg aattttttat caagtttctc caataccctt
    20581 ttttgcattt tttaaattca aaaaaagagc aaccggaaat tctcaaatta gcatcaacaa
    20641 tgcatgcatc atgcattacc aaagtacaat ccgtatttcc aaaaatatct agtactgttt
    20701 taactttatt atctttccat acatcatcct gatcgcataa aaaaataata tcgccttttg
    20761 aatgtactat tgcattttca aaattcttaa tcagtccttt tcgaggacca tctataattc
    20821 taattcttct atccttttca gccaaatcat caacaattct tctggttcta tcagaagaac
    20881 catcatctga aataattatt tcatcttgag aatcaagatt tcttaataca gataaaatct
    20941 gttcctcaat gtatttttcg ccgttaaaag cagcaacaca aaccgaaatc atatttaaca
    21001 aatcctcttt tcttccataa taactttatc attattgctc atacaagcaa aaacagaaaa
    21061 aataaacgga ttatatgcaa tttcaaggag atggtgttca gcaaatgacg ctatagtcac
    21121 gataaaaaat gctaaacaca taatatattt ctcattatcc agcatctttt tttgagccat
    21181 aaatataagc actataacca gcacaaaaac aatagctcca tactcataca tgcatcttac
    21241 aaatgaattg tctataaaaa aataattatc tttagcaata atagacccac catttccgtg
    21301 ttcctcaata tactgccccc ataatttgat attatatgtc gacatagtct gggcaatata
    21361 ctttaatcta tccgacagca catcatttaa tccaataata tatttattat taagctgata
    21421 taaccatccc aatacaaacg aagctatcac aataaaactc tcgcagatat ctacaagtag
    21481 ccattcaata cgcttattta atccaaccca tttttttata atatagcata tagctattcc
    21541 tgtcaagaaa ataagtagac tggcaacagt tgttctacca tcacatctaa aataaagaaa
    21601 agctgcaatc agaacagata ttaaagccat tttccaataa ttcttctttt ttatcacaga
    21661 aaacaatact aaaataatga ataaaacatg agcacaaaaa tccgtaggtg ataaaaatcc
    21721 atatgcaatc ctatttcccc tgtatagact tggatatatt aggttctcaa caatccctat
    21781 ttgacttcct agtatcgtaa ttaataaaaa cggtatacat accattaggt agtcttttaa
    21841 aattctatca attggaatat cttttgctgc tgctatcaat aaacccacat acattaattc
    21901 atgatagcca cttaaaagcc cacttataat aaatggtgca acaattgttg ccataataat
    21961 cagatatttt ctatttggtt caactaatat tttacctata atatatccca gaagcactac
    22021 caaacaaatc catgtataac aacgtggcaa atgaatttgt atcattgttg ttccccaaaa
    22081 gaaatgtata agtagaaaaa tatatacaat agagaagact tctttattat atttttcaat
    22141 aaaagtttta taattaccaa tcaatagcat caattatcac cattattttc tatatattat
    22201 atattcatga tatggaactc tctcctcttc aggtggtgga gtcatatagt ctccatattc
    22261 ttcttccaat acctttttag cctgaacggg aattttggtt tttacacctt caaatataat
    22321 gtcctcaaca tcttcgaacc attccttctc ataatatgta acacagttat cccatatcaa
    22381 atccccgaca tatttgctat tctcatatga atagctattt gtaagcacat caaacttatc
    22441 caaaaaatac ttagttccct tcacatgcga atacaagtaa accggaaatc ttaataatcg
    22501 tttaacaaaa ctcttatttg ttcccattat attcttactc tgtgcacaac caacacactg
    22561 catccaaaat gttttctttt tttctatttg ttttttagca ccattcttaa catttcctaa
    22621 tgcatctaaa ggataaatgt ctataaataa tcccatcccc gcatcagata gtccatccgt
    22681 tttaaccatc ttaaatctag tatcacaaaa tctttgtaaa ttaaaaggat aatcatcggc
    22741 tgtatatttt gacatcaact taaatggata tagttcttct tcatgttcat tacaatatgc
    22801 tacaaactta tcatattcag gtcttaacat gcaaacatcc atatcatcat cccaaggtat
    22861 aaacccatta tgtctcatat atcctattaa tgttccatac ataattgaat acttaatatc
    22921 attgtgctca catatctcat ctattttttt taaaacattt aaggtttctt tatgcacctc
    22981 ttccaaactc agttccctat tcatctaaaa tctgcctttc tacattctcg atatttacaa
    23041 taaccttttt agtttacatc caacatatta catagtcaag aactttgtac ttttactctg
    23101 cttttcttct gttctcttta acaaactccg gcagcacctt aattccaata aacatgacca
    23161 cagcaaaaca taccaaatat cttacaaata tattatcaat aaatataaga tctactattg
    23221 caattatcac aatactaagt aaacataatg caatacagct aactggaata atagaaaatc
    23281 tcattaatct ataagaaata acaagatgta atatcatata acaaatatat gcaaacagcg
    23341 tagtatatgc cgccgcaata aagccatatt tctgtataaa tatataattc gtaatgatat
    23401 taattaccgc cgcactcatt gttccccaca tgataaaact tgttttttgc ttaaaatatt
    23461 cagatggaac tattacatta tatataaata atacaaaagc atctaaaatc atagggattg
    23521 caacatattt tccaactgca tatttttgtc cgcctattat aagaaccaat tctggtgaaa
    23581 ttgacattac tccaatagca ataatgagga aaaaataaca caattgtctt gctctacttc
    23641 tgacagcttc tatttcttta tttttcatct tttcataaaa ccatgtcatc cacacttcag
    23701 aaattgaatc tgatataata gctaaaatca atttgatatt accaacaagt ccatatatgc
    23761 cagcctcaga ctttcctatc attttattaa tcattattct gtcaaattgg gcaagcaaca
    23821 cctgtgatat tccatgtgga ataataggca agctgtattt tattccaaac ttccaatatt
    23881 tttttctaaa tgtcggtcga gcacgtttat ataaatcata tataatatat actgtaacaa
    23941 gtacagtgga aattgtaatt ccaagtaccc ttccaaatcc tcttgatgaa ttaaaaatag
    24001 tcttaattaa tattaaagaa agacctacat ttccaattgt gcttgctaaa gaaaccttaa
    24061 tgtatttttt atactcataa tccaagctaa ttcttgcatt atataaagta actaccgcaa
    24121 ttccaaaact gtatatcaca agcataacca atgtaactaa atcaagatcc aataattttg
    24181 tcaatatttt attaaaaaca atagaaataa acaaggcaac tattaaattt atccaatata
    24241 ttcccataat tgatgatgta aaatcatcaa ttttaccctc aaactcatat tttgcgcttc
    24301 ttattgagct atgcagagcc attccaataa aaacgaacaa gactgcttca tatgcggcaa
    24361 aaaccgtata caaccccatc tcttccgtag ttaacaatct actaaatata ggaatagcca
    24421 aaaaatttat acctttaacc atgacatttc ctatagtata tcctatgcca gccttaaata
    24481 ctttactttg ctttaacttc atatattttc ctcaatttat tttttgttta gtttacaact
    24541 attagtgatt aaggtcaaga cttttataac gtaaatagct tccgacaatt aatgtcgaaa
    24601 gctataatat tatgatatat catatcaatt aaaacttaaa tgtattctcc agtgtctccc
    24661 acttctgatc cttatcactt aaattgattg gtgtgccatc ctcaagtgtg tatccgcttc
    24721 cgtcggcgct tcccggatac tctccgacaa gctgtggcca ctcgatgccg attgccggat
    24781 cattccatgc aagtccgctc tcgtctccaa ggtgccagaa atctgttacc ttgtagcaga
    24841 actctgctac atctgagagt actaaaaatc cgtgtgcaaa gccctccggt ataaggaact
    24901 gcttcttgtt ctcttccgtg agctctactc caaaccactt gccatatgtc tttgaatcgc
    24961 ttcttaagtc tacagccaca tcaaatactg agccttttac tgcacgcaca agctttgcct
    25021 gtgggtattc cttctggaaa tgaagtccgc ggagcactcc ctttgtggag caagactgat
    25081 tatcctgtac gaatactctg tcaatgcctg cctcctgcat gtcctttgaa ttgtatgtct
    25141 ccataaagta tccacggttg tctccgtgta ctgtaggctc gatgatgcaa agtccttcga
    25201 ttccacctac gtttttctgt actttaatct gtcccatgat ttctatgtcc tttctaaaag
    25261 ttaatctcct gcagatatct gtgcagcgca tcctgccatg tagggagcgg cttaaatcca
    25321 ttctc
//
LOCUS       DPUF01000201            3259 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6042, whole
            genome shotgun sequence.
ACCESSION   DPUF01000201 DPUF01000000
VERSION     DPUF01000201.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6042"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="DHV81_07190"
     CDS             complement(<1..470)
                     /locus_tag="DHV81_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCI94239.1"
                     /translation="MNDIKHDIKKRHKNLTEFRYIAVGFFLTILSGAIMLSLPFSSRD
                     GQWTNFLDSLFTATSATCVTGLVVFDTFRHWSIIGQLVILVLIQIGGMGFISIGVAFS
                     MLLHKKIGLRQRDLMQESVNALEIGGIVRLFSVIIRGTFLIEGIGAVLLAIRFIP"
     gene            complement(513..1349)
                     /gene="rsmI"
                     /locus_tag="DHV81_07195"
     CDS             complement(513..1349)
                     /gene="rsmI"
                     /locus_tag="DHV81_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCI94240.1"
                     /translation="MTGTLYLCATPIGNLEDITFRVLRTLKEVDLIAAEDTRNSIKLL
                     NHFEIKTPMTSYHEFNKIDKAYQLVAKLKEGKNIALITDAGTPGISDPGEDIVRICYE
                     EGVPVTSLPGAAACITALTMSGRPTRRFAFEAFLPRDKKERARVIEELKNETRTIIIY
                     EAPHHLIKTVTELYNALGDRELTVCRELTKKHEEKVQTTFSELLERSRTQEPRGEYVL
                     VICGRNVAEIAKEQQESWEAMPLEEHMAHYESQGIDRKEAMKLVAKDRGVSKRDIYRQ
                     LL"
     gene            complement(1363..2121)
                     /locus_tag="DHV81_07200"
     CDS             complement(1363..2121)
                     /locus_tag="DHV81_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCI94241.1"
                     /translation="MEELIHSNERLDDLCRKGYRLIQDPKLFCFGIDAVLLSDYAKVK
                     RGERAVDLCTGNGVIPILLEAKNNGEHYSGLELQPQCADLAKRSVKYNHLEDKVTIEE
                     GDVCNASEIFGRESVEVVTVNPPYMIGQHGIKNADDAMTIARHEVRCTLDDIVRESAK
                     MLKFNGRFYMVHRPFRLAEIFSTMMKYHIEPKRMRLVHPYADREPNMVLIEGLKGGKS
                     RITIEKPLVVYKEPNVYTDEIYEIYGDKPRNNGK"
     gene            complement(2099..>3259)
                     /locus_tag="DHV81_07205"
     CDS             complement(2099..>3259)
                     /locus_tag="DHV81_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94242.1"
                     /translation="LDMAMHVIVETARGIEMGTVLIPPKEVDDDKVVSPLKPVIRVAT
                     DEDEKTVERNKEKEKEAFAICKEKIIKHGLEMKLVDAEYTFDGNKLLFYFTADGRIDF
                     RELVKDLAAVFRTRIELRQIGVRDETKMMGGIGICGRPLCCHSYMADFVPVSIKMAKE
                     QNLSLNPTKISGVCGRLMCCLKYEQDTYEYLNSRMPSVGEKVKTPEGIIGEVKSVSVL
                     RQLVRVVIDNGEEKELREYTVDDLRLKPRQKKDVKLTEEELRELKDLEDSGDETMEDK
                     SQEKPQRNRGHRDNKNEYRERRDKRDNREKRPYRADIEAAEASANETNEVNAANAGRE
                     NKDGYKKPYKGGKNRPNKQKYNRNKDYKNKNYNKEYNKDYNRDKDNGRTDTQ"
BASE COUNT          685 a          903 c          656 g         1015 t
ORIGIN      
        1 ggtatgaagc ggattgctaa aagcaccgct cctatacctt cgataaggaa ggttcccctt
       61 attatcacgc tgaaaagcct tactattcca ccaatctcga gtgcattgac gctctcctgc
      121 atcagatccc tctgtctgag tccgattttt ttgtgcagaa gcatggaaaa tgccacaccg
      181 attgagatga agcccatgcc tcctatctga atcagcacca gaatcacgag ctgccctata
      241 atactccagt gtctgaaagt atcaaacaca acaagacctg tcacacaggt tgcgcttgtt
      301 gccgtaaaaa gtgagtccag gaaattagtc cattgtccgt ctcttgaaga aaacggaagg
      361 ctcagcatta ttgcccctga taatattgtc agaaaaaagc caactgcgat gtatctgaac
      421 tctgtcaggt tcttatgtcg tttttttatg tcatgtttta tatcgttcat atccaatcct
      481 aactatttgc tgcagaatgt attattgtca gctcacagaa gctgtctgta tatgtccctc
      541 ttgctgacac ctctgtcctt tgccacgagc ttcatggctt ccttcctgtc gatgccctgt
      601 gactcatagt gtgccatatg ctcttcaaga ggcatggctt cccagctttc ctgctgctcc
      661 tttgcaattt cagccacgtt tctgccacaa attacaagca catattcgcc tcttggctcc
      721 tgtgtccttg aacgctccaa aagctccgaa aatgtagtct gcaccttttc ctcgtgcttt
      781 ttcgtaagct ccctgcacac ggtgagttct ctgtctccga gcgcattgta aagctccgtg
      841 accgtcttta taagatgatg aggcgcctcg tagattatga ttgtccgggt ctcgttttta
      901 agctcctcaa tgacacgcgc gcgttccttt ttatccctcg gcaaaaatgc ctcaaacgca
      961 aaccttctgg tcggcctgcc cgacatggta agtgctgtaa tgcacgctgc cgccccggga
     1021 agcgaggtaa caggcacgcc ctcctcgtag cagatgcgca ctatatcctc acccgggtca
     1081 gaaatgccgg gggttccggc atcggtgatg agggctatat ttttgccctc cttaagcttt
     1141 gccacaagct ggtaggcttt atctatcttg ttaaactcat gatagctcgt catgggtgtc
     1201 tttatctcaa aatgattgag cagctttatg gagtttctcg tatcctccgc tgcgatgagg
     1261 tctacctcct taagtgtgcg cagcacacgg aatgtgatgt cctccagatt tcctatcgga
     1321 gttgcgcaca gatacagtgt tcctgtcata tgattctcct gtttattttc cgttattccg
     1381 gggcttatcg ccgtatatct catatatctc atccgtgtat acattaggct ctttgtatac
     1441 aactaacggt ttttcgatgg tgatacggct tttgccgccc ttgagtccct ctatgagcac
     1501 catgtttggc tccctgtccg cgtagggatg cacaaggcgc atgcgctttg gctcgatatg
     1561 atatttcatc atcgtgctaa agatctctgc cagcctgaat ggtctgtgaa ccatgtaaaa
     1621 acgcccattg aatttgagca tttttgcgct ctccctgact atatcgtcaa gtgtacacct
     1681 gacctcgtgg cgtgctatcg tcatggcatc gtctgcattt ttgataccgt gctgacctat
     1741 catatacggc ggatttaccg tcaccacctc cacggactct cgaccgaaaa tctccgatgc
     1801 attacacaca tctccctctt ctatggtcac tttgtcctca agatggttat atttcacact
     1861 tcttttcgcc aggtcagcac actgaggctg aagctcaagt ccgctgtaat gctctccgtt
     1921 attctttgcc tcaagaagta tcggaatcac accgtttccg gtacacaaat ccacagctct
     1981 ctcaccgcgc tttacctttg catagtcaga taaaagcacg gcgtctatgc cgaaacagaa
     2041 cagctttgga tcctgtatga gcctatagcc cttgcggcac aaatcgtcca ggcgctcatt
     2101 actgtgtatc agttcttcca ttgtctttgt ccctgttgta atctttatta tactctttat
     2161 tataattttt gttcttataa tccttgttgc ggttgtattt ctgcttgttt ggtctgtttt
     2221 tgccgccctt gtacggcttt ttgtaaccgt ccttattttc tctgccggca tttgcagcat
     2281 ttacttcatt tgtctcattt gcagaagctt ccgctgcctc tatgtccgcc ctgtacggac
     2341 gtttttctct gttgtcacgc ttatcacggc gctcgcgata ttcatttttg ttgtcacggt
     2401 gtccacggtt tctctgcggt ttctcctgtg acttgtcctc catcgtctcg tcgccgctat
     2461 cctcgagatc cttgagctcg cgaagctcct cctcggtgag ctttacatcc tttttctggc
     2521 gcggctttaa gcgcagatca tctacagtgt actcacgaag ctccttctcc tcgccgttgt
     2581 caatgacaac acgcacgagc tgcctgagca cgctgactga cttgacctct ccgatgattc
     2641 cctcaggtgt ctttaccttt tctcctaccg acggcattct gctgttcaga tactcatatg
     2701 tatcctgctc atacttgaga cagcacataa gccttccgca cacaccggat atcttggttg
     2761 gattgagcga gagattctgc tcctttgcca tcttgatgga aaccggcaca aaatccgcca
     2821 tataagaatg acagcagagc ggtctgccgc atattccaat gcctcccatc atcttggtct
     2881 catccctgac accgatctga cgaagctcga ttctcgtgcg gaataccgct gccagatcct
     2941 ttacgagctc cctgaagtca attctgccgt ccgctgtaaa gtaaaacaac agcttgtttc
     3001 catcaaaggt atactctgca tccaccagct tcatttcaag tccgtgcttg atgattttct
     3061 ccttgcagat ggcaaatgcc tctttttcct tttccttatt tctctcaacc gttttctcat
     3121 cctcgtcggt tgccactctg atgacaggct taagcggtga aaccacctta tcatcatcca
     3181 cctccttcgg cggaatgagg acagttccca tctctatgcc tcttgctgtc tccacgataa
     3241 catgcattgc catgtcgag
//
LOCUS       DPUF01000202            4214 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7524, whole
            genome shotgun sequence.
ACCESSION   DPUF01000202 DPUF01000000
VERSION     DPUF01000202.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7524"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="DHV81_07210"
     CDS             complement(<1..279)
                     /locus_tag="DHV81_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94243.1"
                     /translation="MKEIMQTYGAMIVAVVVGIALLSGFGALKNKMSVAFAPQDILSS
                     DSANDSYDSMKNIPVPGIELKEKMRQNAVSYSVYDLLDVTANEDNLDKL"
     assembly_gap    324..386
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(635..997)
                     /locus_tag="DHV81_07215"
     CDS             complement(635..997)
                     /locus_tag="DHV81_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94244.1"
                     /translation="MKTFLGVFLIMFMVVTSSGVLSGFMTVVEAQNLHAQIINELEDS
                     DYDSSVAQTCFENASKAGDTLELKLYMTDNSIRTVTDASQIASSDEIEKARVELKFTY
                     AVAFFGIEQEHVLSGYAL"
     gene            complement(1020..2405)
                     /locus_tag="DHV81_07220"
     CDS             complement(1020..2405)
                     /locus_tag="DHV81_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94245.1"
                     /translation="MTYILILFLTYVLHLLLKLNWVCTGVVLVFLLVMQHFHRIKGQR
                     FQEARKRFLDVSLYIDTLLYSFLKEQKIIRAFEDVKSTLADGHMKETVSRAIDHMMLT
                     FDETEVFVDAMRIIEDEYKCNRIVNAHEFMAHAEYYGGDIKESARILLKDKSAWERRI
                     LRNIEDRQRMFHQIILSVVTSVIISGIILYLPVLSMDISSNIIVQILSAALIVLDDLI
                     ILWGQKFLEVDYLGIDLLPEDDKHAKKLEEYKAYNPAKELRTSILMAVIPALASAFLL
                     YTDRQWAAVAAMGAALICLNQHRIGHRLMKKNLIADVKSAFPKWLMDLALLIQSENVQ
                     VAIQKSREHIPVILKEEVNTLVERLDVEPESSDPYHRFLDCLNLPEINAAMGMLYAVS
                     IGNSGNCGSQIDELITKNLEMLDVADTARLKDKTAGMYLLFLAPVITASFKMIVDMAI
                     FLISFLSYKVV"
     gene            complement(2408..3496)
                     /locus_tag="DHV81_07225"
     CDS             complement(2408..3496)
                     /locus_tag="DHV81_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HCI94246.1"
                     /translation="MELGKEYFGPLWSFVASNEITDIDYNGKEIWLTNIFNERFRANQ
                     QFVTQYMTPAFVEQFTQRIANVVSRQFNKRNPELEAETSELRVTILHESVAKSGRSIS
                     IRKTPPLIRLTAESAIAEKFCSEELLAVLINCVKTKMNITFCGMPGIGKTECVKFFSQ
                     FIPANERVITIEDTLEIRYAETNPGKDCIEMKVSEDVFGYSEAIKSCLRMNPKWIMLS
                     EARSKEVKYLLQSWSTGVRGMTTLHTDDVRNIPDRILNMLESRVDAERMENDIYQALD
                     VGILIRKRRNEDGVVYRYIDQVCFFYRENYTNHVFMLVSDGQIVTHDIPKVILRRFKR
                     EGVNEPFGESFVNEIRMNEAMMNEEREA"
     gene            complement(4078..>4214)
                     /locus_tag="DHV81_07230"
     CDS             complement(4078..>4214)
                     /locus_tag="DHV81_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCI94247.1"
                     /translation="YKKDPDDSVIRIAPTYPSLDELKKAAEVFVVVTRLVSVNKLLEA
                     "
BASE COUNT         1186 a         1058 c          679 g         1228 t
ORIGIN      
        1 aagcttatcc agattatcct cattcgctgt aacatcaagc aggtcatata cactgtagct
       61 tactgcattc tgacgcattt tctcctttag ctcaataccc ggaaccggta tatttttcat
      121 gctgtcatag ctgtcgtttg cgctatcgct gctcaatata tcctgtggcg caaatgccac
      181 actcattttg ttcttcagag ccccaaatcc cgacagcagc gcaattccca caaccaccgc
      241 cactatcatg gctccatatg tctgcattat ctccttcatc tatagtctcc cgattattta
      301 aattgcactg ttaccccata tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnagaa atgcacttgt ggcggccgct gcaatcagca
      421 cgcctccata ctgtctgaat acttctttca tgttctctta cctttacata ctttttaatc
      481 aattcagtta ctatcacagc agagtagaca catacacgag cacaccgatt actccactgc
      541 ctaaaagaag cataataacg cttcctccca attcttctat taaaagctcc atctgttttt
      601 ctcctcttat acagccatat ccacaagaaa agtactacaa cgcatatccg ctcagcacat
      661 gctcctgctc tattccgaaa aatgctaccg cgtaagtgaa cttaagctcc actcttgcct
      721 tttctatctc atctgacgaa gcgatttgtg atgcgtctgt gactgtcctt atggaattgt
      781 ctgtcatata aagtttaagc tccagtgtat ctccggcttt tgatgcattt tcaaagcagg
      841 tctgcgcaac cgagctgtca taatcactgt cctcaagctc attgataatc tgcgcatgta
      901 ggttctgtgc ctcaacgaca gtcataaatc ccgacagcac acctgatgat gtaaccacca
      961 taaacataat caaaaacacc cctaaaaagg tcttcattat ctgactcaca acacaccccc
     1021 tacactacct tgtatgataa aaagcttata aggaaaatag ccatatcaac tatcatctta
     1081 aatgatgctg tgataacagg agccaaaaac aaaagataca tgccggcggt cttatcctta
     1141 agcctcgcgg tgtctgctac atcaagcatc tccagattct tcgtaatcag ctcatcaatc
     1201 tgacttccgc aattgccact atttccaatc gaaacagcat atagcatgcc catggctgcg
     1261 tttatctccg gcaggttcaa acagtcgaga aatctgtgat acgggtcaga gctctccggc
     1321 tccacgtcca gtctctccac aagcgtattt acctcttcct taagtatcac cggtatatgc
     1381 tctctcgact tctgtatggc tacctgcaca ttttcacttt gaatcaatag ggcgaggtcc
     1441 atcagccact tgggaaatgc actctttaca tccgctatca gatttttctt cataagcctg
     1501 tgacctatac ggtgctgatt caaacatata agagctgctc ccatagctgc cacagccgcc
     1561 cattgtctgt cagtatacag taaaaatgca ctggctaacg ccggtataac agccataaga
     1621 attgacgtac gaagctcctt tgccggattg tatgccttgt attcctcaag ctttttggca
     1681 tgcttatcat cctccggcag cagatcaatt ccaagataat ccacttcaag aaatttctgc
     1741 ccccagagta tgattaaatc atcaagcact ataagagctg cagacagtat ctgcacaatg
     1801 atattgctgc tgatatccat gctcaaaacc ggcagataaa gtatgattcc actaatgata
     1861 acagaggtca caactgacag gataatctga tgaaacatcc tctgtctgtc ctctatattg
     1921 cgcagtatcc tgcgctccca tgcactctta tccttaagca gtatcctggc tgattcttta
     1981 atatcaccgc catagtactc agcgtgtgcc ataaactcgt gtgcatttac tattcggttg
     2041 cacttgtact catcttcaat aatcctcata gcatccacaa acacctctgt ctcatcaaac
     2101 gtgagcatca tatgatcaat agccctgctc acggtctcct tcatatgacc atctgccagt
     2161 gtgctcttta catcctcaaa tgcacggatt atcttttgct ccttcaaaaa cgaatacagc
     2221 agggtgtcaa tatacagcga tacatccaga aagcgctttc gcgcttcctg aaatctttgg
     2281 cctttgattc ggtgaaaatg ctgcataaca agaagaaaaa caagtaccac acctgtacac
     2341 acccagttta gcttgagcag aagatgcagc acatatgtca gaaataaaat caatatatac
     2401 gtcattccta agcctccctc tcctcattca tcatggcctc attcatccgt atctcattga
     2461 caaatgactc gccaaacggc tcattcacac cctctctttt aaatctgcga agtatgacct
     2521 ttggtatatc gtgagtcact atctgtccgt ctgatacaag cataaacaca tgattggtat
     2581 agttttccct gtagaaaaag cacacctggt ctatgtatct gtacaccact ccgtcttcat
     2641 ttcttctctt gcggatcaaa attcccacat caagtgcctg atatatgtca ttctccatac
     2701 gctctgcatc cacacggctc tctagcatat tgagtattct gtccggtata ttgcgcacat
     2761 catctgtatg gagtgtcgtc atgccacgca cacctgtgct ccagctctgc agcagatatt
     2821 ttacctcctt tgagcgtgcc tctgagagca taatccactt tggattcatt ctaagacatg
     2881 acttgatagc ctcagagtag ccaaacacat cctcacttac cttcatctct atacagtcct
     2941 tgcccggatt tgtctcggca taacgtatct caagagtatc ctcaattgta atcacgcgct
     3001 cattcgctgg tataaactgt gagaaaaact tcacgcactc cgtctttccg atgcccggca
     3061 tcccgcaaaa ggtgatattc atcttggtct ttacgcagtt tatcagtaca gctaaaagct
     3121 cctccgagca gaatttctca gcaattgcag attctgctgt cagtctgata agcggcggtg
     3181 tttttcgtat gctgatgctc ctgcctgact ttgcaaccga ttcatgcagt atcgtcacac
     3241 gaagctcgct cgtctcggcc tcaagctccg gatttctctt attgaactgt ctgcttacga
     3301 cattggcgat gcgctgtgta aactgctcca caaaagcagg tgtcatatac tgtgtgacaa
     3361 actgctgatt ggctctgaaa cgctcattga atatatttgt aagccatatt tctttgccgt
     3421 tataatctat atccgttatc tcgttgctgg ctacaaagct ccaaagcggt ccaaaatatt
     3481 cttttccaag ctccatgtac cacctcttat ttgacactca tattggcatg atttgaaaag
     3541 tttgtaatca gatcactaaa tgtctttcct actatgcctg ttgcatcaga gcctataacc
     3601 gctgttacaa cgaggataat tgataaaatt gctacaactg ttatgagtat tccgccatat
     3661 tcctgaaaaa tttccttcat aatatttaaa tcctttcttc atctaagtct aaaataatag
     3721 tgttaaataa taaaataata gtgttaaata aaatcaaaaa tcaaatatat gaaactaaaa
     3781 ataaagggaa aaaaatagtg gccgaaatgc cacaaaaaga aacctgcata gaacatatgc
     3841 aagacataat ttaaaaagat atttatatct tacacaaaaa tcgggatttg taaataccaa
     3901 atcccgaaaa aatgtgcaca aatattcatt tagtttttat acatatatca catacttatt
     3961 ttagataaat atgccaatta taagtctacc agttaggaca taacagagaa ttgcatatct
     4021 atccgttaag acataacagt gaattgcacg tctgtccgtt aagacataac agtgatttta
     4081 tgcctcgagc agcttattta cgctgacaag acgtgtgaca acaacaaaca cctctgcagc
     4141 ctttttcaac tcatcaagtg atggatatgt cggagcaatt cttatcacgc tgtcatccgg
     4201 atccttcttg taag
//
LOCUS       DPUF01000203            7354 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_5115, whole
            genome shotgun sequence.
ACCESSION   DPUF01000203 DPUF01000000
VERSION     DPUF01000203.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_5115"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            1..1009
                     /gene="dnaB"
                     /locus_tag="DHV81_07235"
                     /pseudo
     CDS             1..1009
                     /gene="dnaB"
                     /locus_tag="DHV81_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744012.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
     assembly_gap    608..673
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1226..>1308
                     /locus_tag="DHV81_07240"
     CDS             1226..>1308
                     /locus_tag="DHV81_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlaG"
                     /protein_id="HCI94248.1"
                     /translation="MAIEAIKGAGMVYQGSTSTAQIKEQAQG"
     assembly_gap    1309..1493
                     /estimated_length=185
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1494..1710
                     /locus_tag="DHV81_07245"
     CDS             <1494..1710
                     /locus_tag="DHV81_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlaG"
                     /protein_id="HCI94249.1"
                     /translation="QLKKAVDDINKSALADQSEAVFGIHEKTNRVTIKIVDKKTKEVI
                     KEFPPEKTLDMIAKVWEMAGLMVDEKR"
     gene            1720..4342
                     /locus_tag="DHV81_07250"
                     /pseudo
     CDS             1720..4342
                     /locus_tag="DHV81_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515863.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar capping protein"
     assembly_gap    1944..2017
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4366..4761
                     /gene="fliS"
                     /locus_tag="DHV81_07255"
     CDS             4366..4761
                     /gene="fliS"
                     /locus_tag="DHV81_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCI94250.1"
                     /translation="MLQNKAYAAYANNKIMTASPAELTLMLYDGAIKFCNIAIMAVEK
                     EDIQQAHTNIRKVERIIEEFQSTLDDKYPVSKDFNNVYTYLHRRLVEANIKKDKEILE
                     EVLEHLRTMRDAWKEVMQVTCNGNKLHVS"
     gene            4916..5392
                     /locus_tag="DHV81_07260"
     CDS             4916..5392
                     /locus_tag="DHV81_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin biosynthesis protein FlgN"
                     /protein_id="HCI94251.1"
                     /translation="MEQTYIDIMIQSLEKKEQVLDRIIELDIKQKNQLEDPQLTPDDF
                     DEVVEAKSRLIDQLNNLDSGFEKLFERTKEELNGHKEDYKEQIRTMQEHIRSITDKSV
                     KIQSQEARNKDLMTLKFASIKKQAREVRVGTQAASRYYKSMSGTGYMEPQFMDNKK"
     gene            complement(5403..6161)
                     /locus_tag="DHV81_07265"
     CDS             complement(5403..6161)
                     /locus_tag="DHV81_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94252.1"
                     /translation="MKKNIYLIIITIITVVCIIAGSLYHIGGFALGLFDNLIPRSDKS
                     LGNVCTEELSVDEFSNLVFDTTISNINVKTGDSYTVSYKCNKRLVPKIRSTGDTLTIS
                     QSKGANYKRNTTSEITVTIPEGTALNKLSLDTGVGEVNLNSLTASDAEFDTGVGDLYV
                     TDCSFATCDVDGGTGNLSFENCAFDEMDIDGGTGNITVTSSQSLDGYMMDLDSGTGDI
                     TINGNDYDDEYEVNEHAKKHLVIDSGLGDIEVKY"
     gene            complement(6255..7262)
                     /locus_tag="DHV81_07270"
     CDS             complement(6255..7262)
                     /locus_tag="DHV81_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94253.1"
                     /translation="METIKNYLENMFSHLPNTPEVQKAKYELYQMMEDKYNELISEGK
                     SDNEAIGIVISEFGNLDELADSLGIKSFVNPSQAMPAAKTLSRETATAFLRDSAKQAY
                     LTAFGVLLCIIASLGPIFSECIPRSLASPDASDAIGITFLFLCVAVAVGFFIFSGSIS
                     SKWSYLKQEPYCIDFETANWVIERKESYRSTHAMLLTVGIMLCILCAVPAIIISSLNT
                     KSTFADSLSGGLVLVFIAIGVFMIVFTNMKKSSFDKLLSLNGAQTMGGNFANSHDGKV
                     YYENPVVAAIMSVYWSTVTCIYLCWSFITFDWGITWIIWPIAAIINSLVENLLGDKHG
                     N"
     gene            complement(7275..>7354)
                     /locus_tag="DHV81_07275"
     CDS             complement(7275..>7354)
                     /locus_tag="DHV81_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCI94254.1"
                     /translation="GREYYRMKCEEWKLTLDVVSKFIRF"
BASE COUNT         2199 a         1479 c         1693 g         1658 t
ORIGIN      
        1 tcgtaaaaga aaatgccata aagcgaaagc tcatacatgt gacagaggac ctagagaacg
       61 agtgctacgc cggtaaggaa tcgctcgaga gcgtgctcga caagacagag cacgatgtat
      121 ttgagctgct ttccacaagg cagacaggtg actacgtgcc aatccgtact gtagtcatga
      181 atgccctcga gaaaatcgaa aaagcagcac agcaggaggg caccgtaacc ggaataccga
      241 caggcttcat agacttagac tacaggacag caggacttca gccgtcagat ctcgtgctcg
      301 tggcagcccg tccgtcaatg ggaaagacag ccttcgtgct caacatagca cagcatgtgg
      361 catttcatgc acatctgtgt acagccatat tctcactcga gatgtcgaag gagcagctcg
      421 taaaccgtct gttctcactc gaatcaaagg tagatgcgca ggcactgcgt acaggaaatc
      481 tgtctgatgc cgactgggaa aagctggtcg agggagccgg aatcatcgga gactccgagc
      541 tcatcataga cgacactccg ggtatcagca tatcagagct tcgaagcaag tgccgtaaat
      601 acaagctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnngaatcgc gtcagcagga aatctcagac atatcacgtt cgcttaaggc
      721 actcgccaga gagctttcag tccctgtagt ggccctctcc cagctttcac gagctgtaga
      781 gcagcgaccg gaccacaggc ctatgctttc tgatcttcga gagtcaggag ctatcgagca
      841 ggatgccgac gtcgtcatgt tcatctatcg tgacgattac tacaacaagg acaccgagct
      901 caagggtatc tcagaaatca tcatagctaa acagcgtaac ggtccaatcg gcaccgtaaa
      961 cctcgcatgg ctgcctgagt acacaaagtt tgcaaatctg gagcattaag actaaaacac
     1021 aaaaagaata aagagacaat ccatcccggc tcacgtcata ggataaagtt aagtatcctg
     1081 atgtgggccg ggatttttca aaatctcttt atacttaaaa agagaagaaa gctataaact
     1141 ttcccgcaat tcatccgata tattccttag aatagggtaa gaacaacagt caattgcaac
     1201 atattttcac acggaggtgt tagagatggc aatagaggca atcaagggtg caggaatggt
     1261 ctatcaggga agtacatcaa ctgcacagat aaaggaacag gcacagggnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcagctt
     1501 aaaaaggctg tggatgatat caacaagagc gcgttggctg accagtcgga ggcagtgttc
     1561 ggcatccatg agaagactaa ccgcgtgaca ataaagatag ttgataagaa gacaaaggaa
     1621 gtcatcaagg agtttccgcc ggagaagacg ctcgacatga tagcgaaggt ctgggagatg
     1681 gcaggactca tggtagatga gaagagatag gaggacacca tggcaatcag attatcggga
     1741 atgacatcag gcctggatac agattctatt gtaaaggcaa tggtatcgtc atataccacc
     1801 agaaaagaga aatatcagaa ggcgcagact aagcttgagt ggacacagga tgcgtggaaa
     1861 tcccttaata ccaaggtgta cagcttgtac agcaatattt ccaatctgcg attttctaca
     1921 gcatacaact taaagaagac atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc acacagtcgc tgaaaatcaa
     2041 gcagatggca aaggccggat atatgaccgg agcagaaatt aaggcagaca gtgagaagac
     2101 tacacttgca cagctcggtt acacaggcgg tgatgccaag cttgaggtaa agatggggga
     2161 cgagaccaag tctatcactc tcagcgcatc atcaaccatg aacgacgtgg taaaggaact
     2221 caggaatgcg gggcttgatg caaactacga ttcaagctac aagagatttt atatcagctc
     2281 aaaaaagaca ggcgtggcaa acgattttac cataaccggc agcaatatag atggagcatc
     2341 agcactttat agcctgggaa tagcatatgg tggtgaaaag accactaccg gtacttattc
     2401 atccaatcca tatgatgagt atgcagcttt acatggagtt tcagacgatg agaccagagc
     2461 caaaatcgag gcagctgtta caaactacaa taatctgaca acagatgcgg cgaagatgaa
     2521 tgcgcagagc aaaaatctta tgaatgccgt aaattatggc tactcgtatg tcacacttca
     2581 gggcatttat aagaatcttg atgaaaaaaa tgcaggctat agtgataagc tgaagctgct
     2641 tgagaagctt ggctcatctc gtgatggcgc agtgatttca aagaatggtg acagctatga
     2701 tacatataca aagaccacgc tgacagatac aaagggtaac actctttata agtatattga
     2761 cagcgacaac aatagccact atattgcagc tgttgctgat acggacaagg gtaccacatc
     2821 gtactatgat gtgacagaat ctacgaggac agcttatacg gacgctgatg gcaaggagct
     2881 tgaagcggta cctacaggag atggtgatac aacactgaaa gtcggtgata agacttacaa
     2941 aaaggacgag gcagacggca agtactatct gcaaaaggaa gatggcacag ctgataaaac
     3001 tgattccatc actgtttcac aaaagactga gtatactatt gcggcagatg ccgtaaacaa
     3061 gactggcata gagtcagctt cagtagcgta tgacacaatc atcaagtcaa tggatgaaaa
     3121 gactaaaagc tcatacgatg aggttcttgc gtcagttcag gcatttgaga attcaaagga
     3181 cgaagtgcta ggaagcgatg acaagtactc ggttgcaagc ctcaccaagg ctattcagaa
     3241 tgcttatgca ggcaatggaa aggaagccgt agagcagctt ttagcaggaa acggaaacga
     3301 ggctgacggc tttgtaaata aagccaatgc tctggaggag gcagcagagg ccaacacaaa
     3361 aaaagcagca gatagcaata ttgtaaagga gcttgcctca aagcagaagg attcggctga
     3421 gtatacacag gcgctaaacg acctgctcga cagggtaaac actgcataca cgcttcagaa
     3481 taaggcactt ttcaattcac aggtgaagaa ggtcgcagga caggattcgg agattgagct
     3541 gaacggtgta aaatatacag gtgacagcaa cagctattca atcaatggac tgacaatcga
     3601 ggcacttgct gagaccggag acagtgctat aagcattaca accagcacag atacacaggg
     3661 tgtatatgat aaggtaaagg atttcctgac agagtacaac aacatcatca acgagatgac
     3721 gaagctatac aatgctgatt cagcaggaag ctacgagccg ctcacggatg atgagaagga
     3781 tcagatgtct gataaagaga ttgagaagtg ggagacaaag attaaggact ctctgcttcg
     3841 cagggattct acacttggca gcgtaatgac agcaatgcag acagccatga tgtcatcagt
     3901 tgagataggt ggcaagaagt actcgctctc aagctttgga atcagtacac ttggattttt
     3961 gaatgcagcg gagaatgagc agaatgcata tcacattgat ggtgatgagg atgacgccaa
     4021 tacatcaggc aagacagata agctcatggc agctataaac gatgatccgg atacggtaat
     4081 gcagtttatg cagcagcttt caactaatct gtatagtgca atcggtgaca agatgaagtc
     4141 tacagatttg agctcagcat ttaccatcta caatgacaag cagatgaaga cagagtacac
     4201 taactacacc aagactatta aggaatggga gaaaaaaatc tcagatcgtg aggattacta
     4261 ctacaacaag tttactcaga tggagtcagc tatgaccaag ctcaacagca cacagagctc
     4321 aattagcagc tacttctcat aaaaagatac cagggagggt atattatgtt acaaaataaa
     4381 gcatacgcag catatgcaaa caacaaaatc atgactgcat caccggcaga gcttacactc
     4441 atgctgtatg atggtgcaat caaattttgc aatattgcaa tcatggcagt ggagaaggag
     4501 gatatccagc aggctcacac aaacatccgc aaggttgagc gtatcataga agagttccag
     4561 tccacacttg atgacaaata tccggtatca aaggatttta acaatgtata tacatatctt
     4621 cacagacgtc ttgtcgaggc aaacataaag aaggacaagg agattcttga ggaggtgctt
     4681 gagcacctgc gcactatgcg tgacgcgtgg aaagaggtca tgcaggtcac atgtaacgga
     4741 aacaagctgc atgtatcata gataaatgta tcataaatca ccgacaggga ttatagagag
     4801 tttttaactt aaacaaaacg cagagacagc agcatgaagc tagtttgttt gtgcgttttg
     4861 tgctatcata gagatgatta tagttaaaaa caatcgaaaa tacaggggga acacaatgga
     4921 acagacatac atagacataa tgatacaaag cctggagaaa aaggagcagg tactcgaccg
     4981 gataatagag cttgatatca agcagaagaa ccagctcgag gatccacagc tcacacctga
     5041 cgacttcgac gaggtagttg aggcaaagag caggcttatc gaccagctca ataatttaga
     5101 ctcaggcttt gagaagcttt ttgaacgtac aaaggaagaa ctgaatggtc acaaagagga
     5161 ctacaaggag cagatacgca ccatgcagga gcatatccgc agtatcacgg acaagagcgt
     5221 aaaaatacag agtcaggagg cccgcaacaa ggatctcatg actctgaaat ttgcgtccat
     5281 aaagaagcag gcccgcgagg tgcgtgtcgg cacgcaggcc gcaagccgct actacaagtc
     5341 catgtcagga accggatata tggagccgca gtttatggac aataaaaagt gaaaagttta
     5401 atttaatatt tcacctcaat atccccaagc cctgagtcga taaccaggtg ctttttggca
     5461 tgctcattta cctcatactc atcatcatag tcattgccgt tgattgtgat atcgcctgta
     5521 cctgagtcca ggtccatcat gtaaccgtca agtgactgtg aggatgtcac agtgatgtta
     5581 cctgtaccgc cgtcgatatc catctcgtca aaggcacagt tttcaaagct aagattgccg
     5641 gttccgccat ccacatcaca tgtggcgaag ctgcagtctg tgacatataa atcgcctaca
     5701 ccggtgtcaa attctgcatc ggatgcggtc agggaattaa ggtttacctc gcctacaccg
     5761 gtatcaagtg agagcttgtt caatgctgtg ccctcaggaa tggtaacagt gatttcactt
     5821 gtggtgtttc ttttgtagtt ggctcctttg ctttgggata ttgtgagggt atcgcctgtg
     5881 ctcctaatct ttggaaccag tcgcttgtta catttgtatg agaccgtgta gctgtcaccg
     5941 gtttttacgt tgatgttcga tatggttgta tcaaatacca ggtttgaaaa ttcatcgact
     6001 gaaagttcct ctgtgcatac gtttccgaga gacttgtctg agcgcggaat cagattgtca
     6061 aacaggccta gggcaaagcc cccgatatgg tagagactcc cggcgatgat gcagaccacg
     6121 gtaattattg taattattat tagatatata ttctttttca tgacttgtct ccatttctga
     6181 attatcttgt tgcataattt gttttgtgat tattcaaaat atgctataca catttttgac
     6241 tgtcctggtt tccattaatt tccatgttta tcgccgagca ggttttccac cagcgaattg
     6301 atgattgcgg ctattggcca gataatccat gtgatacccc agtcaaaggt aataaagctc
     6361 cagcatagat aaatgcatgt gacagttgac cagtagactg acatgattgc agccacaaca
     6421 gggttttcat agtacacctt gccatcgtgt gaattggcaa aattaccgcc catagtctgt
     6481 gctccgttca gggaaagaag cttgtcaaag cttgatttct tcatgtttgt gaatactatc
     6541 atgaatacac cgattgctat aaacacgagc acgagtccgc ctgaaagtga gtctgcgaag
     6601 gtgcttttgg tgttcagtga gctgattatg attgccggca ctgcgcagag tatgcagagc
     6661 atgataccca ctgttaaaag catcgcatgt gtggagcggt agctttcctt tcgctcgatg
     6721 acccagtttg ccgtctcaaa atctatgcag taaggctctt gcttcaggta gctccacttg
     6781 gatgagatag agcctgaaaa aatgaagaag ccgactgcca ctgcgacaca caggaacagg
     6841 aaggtaatgc cgattgcatc agatgcgtcc ggtgaagcga gtgaacgcgg aatgcattcg
     6901 ctgaatatcg gtccaagact tgcgattatg caaagcagca ctccaaacgc tgtgagatat
     6961 gcttgttttg ccgaatccct gaggaatgcg gtagcagtct cgcgggagag tgttttggcg
     7021 gcaggcattg cctgactggg attcacgaag ctcttgatac cgagtgagtc ggcgagctcg
     7081 tcaagatttc caaactctga tatgactatg cctatggctt cgttgtcaga tttgccctcg
     7141 gatattaatt cgttgtattt atcctccatc atctgataga gctcatattt ggctttttgc
     7201 acctcgggtg tattcgggag gtgcgaaaac atattttcca gataattctt tatagtttcc
     7261 ataaaatgtc tcctttaaaa tcgtataaat ttgcttacta catcaagtgt cagcttccat
     7321 tcctcgcatt tcatcctgta atattctctg ccgg
//
LOCUS       DPUF01000204            4211 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_35383, whole
            genome shotgun sequence.
ACCESSION   DPUF01000204 DPUF01000000
VERSION     DPUF01000204.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_35383"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="DHV81_07280"
     CDS             complement(<1..1750)
                     /locus_tag="DHV81_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter"
                     /protein_id="HCI94255.1"
                     /translation="MNIYSILQLCGGLAFFLFGMHVMSGSLEKLAGGNLQKILKEVTS
                     NPIKSLFFGAIVTIAIQSSSALTVMLVGLVNSGIMELAQTVGVIMGSNIGTTLTAWIL
                     SLSGIESDNLFISMLKPENFSPIIALIGVIMIMGSKKEKRKDVGIIMAGFAVLMYGMQ
                     LMSGAVSPLADMPEFTGLLTTFNNPFLGVLVGAVFTGVIQSSAASVGVLQALAMTGAI
                     SYGMAIPIIMGQNIGTCVTALLSSIGVNKNAKRVSIIHISFNLFGTAIGLVVYCIARY
                     AVDLALFNDSITPVMIAVFHSIFNIATTIILLPFSNTLVKIAKKLVTTDNADGQVVLD
                     ERLLLSPGLAVKECLEKTNEMAELARDSFKNALDLFDNYSDSKFDDIEVMEERLDYLE
                     DQLDTFLIHLSGKDVSEGGNNEISKMLHAINDFERIGDHAINMAKLAKQIDDNKLEFS
                     KNARKELTVLNNALREILTLTVEAFSKNDLTEAVKVEPLEQVIDDLTKEIRNYHIERL
                     QKGKCDSRLGVFLTDYITNCERASDHCSNIAVCLIQTHNSSFETHDYLNELKAGQEPA
                     FVGQFTMYQDKYHLDED"
     gene            2039..3637
                     /locus_tag="DHV81_07285"
     CDS             2039..3637
                     /locus_tag="DHV81_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCI94256.1"
                     /translation="MSDVKSVIESGRAVLGIEFGSTRIKAVLIGEDHAPIATGAHDWE
                     NMLVDGIWTYSLDMIWEGISDCYSKMAQDAKEKYGVTIKKLAAIGISGMMHGYLPFDK
                     EDNLLVPFRTWRNTITGEAAAELTEVLGFNIPQRWSIAHLYQAILNKEEHVSKISYFT
                     TLAGYIHWQLTGEKVMGVGEASGMFPIDSKTRDYDVAMLDKFAALDKVKEYPWDIRDI
                     LPKVLTAGEKAGTLTEEGAKRLDVSGNLESGILLAPPEGDAGTGMVATNSVAVRTGNV
                     SAGTSVFAMVVLEKALKAVHEELDMVTTPSGDAVAMVHCNNCTSDLNAWVGLFKEFSE
                     LFGMDIDMNDIYGKLYNNALTADKDCGGLVAFNLFSGEPVIGLNEGRPMFARKPDARM
                     NLANFMRTHLYASLATLKIGCDILFKEEKVKVDTLYGHGGLFKTKGVGQSILAAAMDA
                     PVAVMETAGEGGPWGMAVLAAYMVNKADGEPLEKYLSDRVFNGEKGIVMQPDPDDVKG
                     FDEYINTYKAAIEAERAMVKALPL"
     gene            3671..4211
                     /gene="araD"
                     /locus_tag="DHV81_07290"
                     /pseudo
     CDS             3671..4211
                     /gene="araD"
                     /locus_tag="DHV81_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741574.1"
                     /note="catalyzes the isomerization of L-ribulose
                     5-phosphate to D-xylulose 5-phosphate in the anaerobic
                     catabolism of L-ascorbate; links the arabinose metabolic
                     pathway to the pentose phosphate pathway and allows the
                     bacteria to use arabinose as an energy source;
                     frameshifted; too many ambiguous residues; incomplete;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
     assembly_gap    3916..4008
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1216 a          814 c          992 g         1096 t
ORIGIN      
        1 aatcctcatc aagatgatat ttgtcctgat acatggtgaa ctgacctaca aatgcaggct
       61 cctgtccggc cttaagctca ttgagataat catgtgtctc gaatgatgag ttgtgtgtct
      121 gaatcaggca tacagcaatg tttgagcagt gatccgaagc tctctcacag tttgtgatgt
      181 aatctgtgag gaatacgccg agtctgctgt cacatttgcc cttctgaaga cgctcgatat
      241 gataatttct aatctccttg gtgagatcgt caataacctg ctcaagcggc tcaaccttga
      301 ctgcctctgt caaatcgttt ttgctaaatg cctctacagt gagagtgaga atctcgcgca
      361 gagcattgtt gagcaccgtg agctcttttc ttgcgttctt tgaaaattca agcttgttgt
      421 catcaatctg cttggcaagc tttgccatat ttatcgcatg atctccgatt ctctcgaagt
      481 cattgattgc atgaagcatc tttgaaatct cgttgttgcc gccctcagaa acatcctttc
      541 cggataagtg aatgaggaag gtgtcaagct gatcctctaa gtaatcaagg cgctcctcca
      601 tgacttcaat atcatcaaac tttgaatctg agtaattgtc aaagagatct aaagcattct
      661 tgaagctgtc tcttgcaagc tcagccatct cgtttgtttt ctcaagacat tctttgacag
      721 caagtccagg tgagagtaaa agtctctcgt cgagtacgac ctgtccgtct gcgttgtcag
      781 ttgtgacaag cttcttagca atctttacaa gagtattgct aaacggaaga aggataatag
      841 tggtagcgat gttgaagatt gagtggaaca ccgcaatcat tacaggagta atgctgtcat
      901 taaataatgc cagatcaaca gcataacgtg cgatacagta tactacaagt cctattgctg
      961 taccgaacag attgaagctg atgtggatga tggatactct cttggcgttt ttgtttacgc
     1021 cgattgacga aagcagtgca gtcacgcatg taccgatgtt ctgacccatg atgatcggga
     1081 tagccatgcc atatgagata gcgcctgtca tggctaaagc ctgcagcaca ccaactgatg
     1141 cggcagatga ctggatgact ccggtaaata cagcacctac aagcactccg aggaatggat
     1201 tgttaaaggt ggtgagcagt ccggtaaatt caggcatatc tgcgagtggt gatacagcgc
     1261 cgctcatgag ctgcatacca tacatgagta cggcaaaacc tgccatgatg attccaacgt
     1321 ccttgcgctt ttccttcttg cttcccataa tcataatcac accgataagc gcgatgatag
     1381 gggagaagtt ctcaggcttt aacatcgaga tgaacagatt gtcactttcg atacctgaaa
     1441 gactaagaat ccatgcggtg agagtggttc cgatgttgga acccatgatg acaccaacag
     1501 tctgggcaag ctccatgatg ccggagttta cgagtccgac aagcataaca gtaagtgcgg
     1561 atgaggattg gattgcaata gtgactattg ctccaaaaaa taagctcttg atagggtttg
     1621 aggttacctc cttaagtatc ttttggagat tgcctcctgc gagcttctca agactgcctg
     1681 acataacatg cattccgaaa aggaaaaagg caagtccacc gcataactgc aatatagagt
     1741 aaatgttcat gtttacctcc gtttgtaaaa ataatttgtt ttactatttg ctccagttat
     1801 acatatttag tttacacaaa acacatataa cataacataa tttttacatt ctatcaatat
     1861 aatctgaaat tttaataaaa aaagtgatat tcacttttga acacttttga aaacgtttta
     1921 aaatttatgt atatttatgt tttcttgtgt ttacttttga aggaaaacgt tgtacaatta
     1981 cagtataata atttaaggta attattcaga aatttacatt acaaggtgga ggtatattat
     2041 gtcagacgta aaaagtgtta ttgaaagcgg cagggctgtg cttggaatag agttcggttc
     2101 taccagaata aaggcggtgc ttatcgggga ggatcatgct cctatagcca caggtgccca
     2161 tgattgggaa aacatgcttg tggacggcat ctggacatac agcctggata tgatttggga
     2221 gggcatttca gactgctaca gcaaaatggc gcaggatgca aaggaaaagt atggtgttac
     2281 catcaaaaag cttgcggcaa tcggtatttc cggcatgatg cacggctatc tgccttttga
     2341 caaggaggat aatctgcttg tgccatttcg gacatggaga aataccatca caggtgaggc
     2401 tgcagcagag cttactgagg tgcttggctt taatattccg cagaggtgga gcatagcgca
     2461 tctgtatcag gctatattaa ataaagaaga gcatgtatca aagatatcat atttcacaac
     2521 attggcaggc tatattcact ggcagctcac aggtgaaaag gtcatgggtg ttggtgaggc
     2581 atcaggcatg tttcctatcg attctaagac cagggattat gatgtggcaa tgcttgataa
     2641 atttgcagca ttagataagg taaaagaata tccgtgggat ataagagaca ttctgccaaa
     2701 ggtgcttacg gctggagaaa aggcaggtac gctgacagag gaaggagcta aaaggcttga
     2761 tgtaagtggc aatctggaga gcggtatatt gcttgcaccg cctgagggag acgcaggcac
     2821 gggaatggtc gcaaccaatt ctgttgcagt gcgcaccgga aatgtatcag cgggcacatc
     2881 tgtatttgct atggtggtgc ttgaaaaggc gcttaaggca gttcatgaag agcttgatat
     2941 ggtcaccaca ccatcgggag atgcggtagc tatggtgcac tgcaacaact gtacgtcaga
     3001 cttaaatgca tgggtgggat tattcaagga attttcagag ctgtttggca tggatataga
     3061 tatgaatgac atatatggaa agctctacaa caatgcactt acagccgata aggactgtgg
     3121 aggccttgtt gcctttaacc ttttctcggg agagcctgtc atcggactca acgagggcag
     3181 acctatgttt gccagaaagc cggatgcaag gatgaatctt gcaaacttta tgcgcacaca
     3241 tctctatgca agtcttgcaa cactaaaaat cggctgtgat attctcttta aagaggaaaa
     3301 agtaaaggta gacacacttt acggtcatgg tggtctgttc aagacaaagg gtgtgggcca
     3361 gagcatactt gcagcagcca tggatgcacc ggttgcggtc atggagacag ccggagaggg
     3421 cggcccgtgg ggcatggcag tgctcgcagc ttatatggtg aataaagcag atggagagcc
     3481 tttggaaaaa tatctttcag acagggtatt taatggcgaa aagggcatag taatgcagcc
     3541 ggaccctgac gatgtaaagg gctttgatga gtacataaat acatataagg cagcaattga
     3601 ggcagagcgt gccatggtaa aggcgcttcc gctgtaatag cagtaaaaaa taataagaga
     3661 ggaacaaaaa atgcttgaag aattaaagaa acaggtttac gaagcaaaca tggagctccc
     3721 aagaagaggg cttatcacat atacatgggg caatgtcagt ggaattgaca gagaaacagg
     3781 ctattttgtc atcaagccaa gtggagtgga ttatgatgtt ctcacccctg atgatatggt
     3841 agtcatggat cttgaaggaa ataagataga gggaaaatac aagccttcgt cagacactgc
     3901 aacacactta gagctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cggcagggat
     4021 attccgctct acggcaccac tcacgcagac tatttccttg gctcaattcc atgtgcgaga
     4081 aatctcacca cagaggaaat caacgaagcc tatgagaaaa acacaggcct tgtcatcata
     4141 gagacatttg agaacagaaa aatcaatcct gtatacacac cggctgtgct ctgtcagaat
     4201 cacggaccgt t
//
LOCUS       DPUF01000205            5250 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4734, whole
            genome shotgun sequence.
ACCESSION   DPUF01000205 DPUF01000000
VERSION     DPUF01000205.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4734"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            26..1139
                     /locus_tag="DHV81_07295"
     CDS             join(26..100,102..1139)
                     /locus_tag="DHV81_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271338.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCI94257.1"
                     /translation="MVELDNFKSTLQGYKAPLQEVRDSLDLANKEEKIQELEREMEAP
                     DFWDDPEKSQSKMKELKSMKDDVATYAKLKEQYEDIETMIEMGYEENDESLIPEIQQM
                     LDDFESTYDGIRMKTLLSGEYDKCNAILRLNAGAGGTESCDWASMLFRMYSKWADKKG
                     FTLEVLDSLDGDEAGIKSITFQVNGENAYGYLKSEKGVHRLVRISPFNAAGKRQTSFV
                     SCDVMPDIEEDLDIEVKDEDIRIDTYRSSGAGGQHINKTSSAIRITHFPSGIVVQCQN
                     ERSQHMNKDKAMQMLKAKLYLLKQEENAAKEAGIRGEVKEIGWGSQIRSYVLQPYTMV
                     KDHRTDVETGNADAVLDGNIDIFINGYLKWLATRSE"
     gene            1150..1905
                     /locus_tag="DHV81_07300"
     CDS             1150..1905
                     /locus_tag="DHV81_07300"
                     /inference="COORDINATES: protein motif:HMM:PF04280.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94258.1"
                     /translation="MGLVIAVAILAVLTAAGVTVYIKVRRLSESLLGTPDVTEGINRI
                     RENVSTTPKSVSGMTRLMEPQIKRDFPEFVWEQFKRMSERVLVSALCAITTEDIYKLD
                     REASDEVRQQVLVRIDSNEAAGFTEHFDEIRVHQTEISNYVKRDGRCVISIQSAVEYF
                     YYKTASGKLISGDKEYKKQTRYNMELVYIQDIDMLDNAGIGSAVGTTCPNCGAPVRSL
                     GAKKCEYCGSYVEPVNIKVWKLQSFHEVDYNHI"
     assembly_gap    1911..2118
                     /estimated_length=208
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2119..3171
                     /locus_tag="DHV81_07305"
     CDS             <2119..3171
                     /locus_tag="DHV81_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HCI94259.1"
                     /translation="YYKVSNSSMPSLFNGQFGDSLKETFDRVRFGGQTPQSQKVYYIN
                     LQEIKGIKFGTRNPINYYDTFYNAELFLRAHGTYSIKIVNPLQFYTEAIPKNMDHVEI
                     TDINEQYLSEFLEALQSAINQMSADGTRISFVTSKASELGRYMAKCLDESWNQMRGME
                     VQSVGIASLSYDEKSQELINTRNEGAMLSDPSIAQGYMVKNMSEGVKNAGSNSGGAMQ
                     GFMGVGMGMSTMGNMMNGFTQMAQNNRSAGMNAPGNGAAGMAAGVASGATQQEDASGW
                     TCECGQKNTGKFCSNCGKPMPQEITEWTCECGQKNTGKFCTNCGKPLPVNTEWTCECG
                     QKNTGKFCSNCGKPRA"
     gene            3269..3712
                     /locus_tag="DHV81_07310"
     CDS             3269..3712
                     /locus_tag="DHV81_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCI94260.1"
                     /translation="MAEKSNYFVLRAKAVPEVLLKVVEAKRLLDSGKLESVQEATDAV
                     GISRSSFYKYKDDIFPFYEGRKGKTVNLVIQMDDEQGILSVVLKSVADAKANILTIHQ
                     SVPVNGIASLTLSVDVFPESDDVDDMAKQIESLKGIHYVKILARE"
     gene            3720..4910
                     /locus_tag="DHV81_07315"
     CDS             3720..4910
                     /locus_tag="DHV81_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCI94261.1"
                     /translation="MKIAVMGYGTIGSGVVEVLEINKEKIAKRAGEPIEVKYVLDLRE
                     FPGTPIENKIVHDYKVIAEDPEIGIVVETMGGVEPAFTFVKAMLEAGKQVATSNKNLV
                     AAKGAELIKVARDHGVNFQFEASVGGGIPIIRPLNKCLTADEIEEITGILNGTTNYML
                     TKMTEEGADFDEVLKDAQDKGYAEKDPTADIEGYDPCRKIAILTSLVAGQQVDFEDIH
                     CEGITKITPEDIKYAKAMHRAIKLLASSRKVGDSYTCLVAPYMIDDTHPLSGVNGVFN
                     GVFLRGNVLGDAMFYGSGAGKLPTASAVVADVVDMTKHQNTNIYIDWKPEKLTLVSYD
                     DSVNSFFVRTDSPKSEVEAVFGHVDYIEDVVDGEYAFTTGDMTEKDFADKASKINVIN
                     RIRL"
     gene            4922..>5250
                     /locus_tag="DHV81_07320"
     CDS             4922..>5250
                     /locus_tag="DHV81_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body and hook protein"
                     /protein_id="HCI94262.1"
                     /translation="MVKGLYTAHTGMVNEMKRMDVLANNLANSDTYGFKKEGTTSHSF
                     KNELAIKIKDTSNYGLHKNLGSMTYGVHLGETYTDWDEGSFKVTDNPTDLAIGGKGFF
                     AVAFTDKS"
BASE COUNT         1581 a          899 c         1334 g         1228 t
ORIGIN      
        1 aaaatgcaaa gaaagaggtg attctgtggt agagttggat aatttcaagt ccacgctcca
       61 aggctacaaa gccccactac aggaagtgag ggattcactt tgacctggct aataaagaag
      121 aaaaaatcca ggaattagag cgagaaatgg aagctcctga cttctgggat gacccggaga
      181 agtcacagag caagatgaaa gagcttaaga gtatgaagga tgatgtagct acatacgcaa
      241 agctcaagga acagtacgaa gatatagaga cgatgattga gatgggctat gaggagaacg
      301 atgagtcact catacccgag atacagcaga tgcttgatga ttttgagagc acatatgacg
      361 gtatcagaat gaagactctt ttgtcgggag aatatgataa atgcaatgcg attctgaggc
      421 tcaatgcagg tgcaggtggt accgagtcgt gtgactgggc gtccatgctg tttcgtatgt
      481 actcgaagtg ggctgataaa aagggcttta cccttgaggt gctcgattcg cttgatggcg
      541 atgaagccgg tatcaagtcc attactttcc aggtaaacgg agagaatgca tatgggtatc
      601 taaagtctga aaaaggtgta cacaggcttg ttcgtatttc gccgtttaat gcggcgggaa
      661 aaaggcagac ctcattcgta tcatgtgatg tcatgcctga tattgaggag gatctcgata
      721 ttgaggtgaa ggacgaggat atcagaattg atacctaccg ctcaagtgga gcaggaggac
      781 agcatatcaa taaaacttcg tctgctattc gtatcacaca ttttccatca gggatcgtgg
      841 tacagtgtca gaatgagcgt tcacagcata tgaacaagga caaagccatg cagatgttaa
      901 aggcaaagct gtatctttta aagcaggaag agaatgctgc aaaggaggcg ggcatcagag
      961 gcgaggtcaa ggaaattggc tggggaagcc agatacgttc gtatgtgctt cagccttaca
     1021 ccatggtcaa ggatcacagg acagatgtgg agaccggtaa tgcggatgca gtgctcgatg
     1081 gcaatataga tatatttatc aatggttatc tgaagtggct tgcaacacgt tcagaataag
     1141 gaggcaaatg tgggtttagt tatagctgtg gcgatattag cagttctgac agctgccgga
     1201 gtgaccgttt acattaaagt cagacggttg tctgaaagtc ttcttggtac acctgatgtc
     1261 acagagggca tcaatcgtat cagggagaat gtctctacga ctcccaagtc ggtttcaggc
     1321 atgacgcgcc ttatggagcc acagataaaa agggattttc cagagtttgt ctgggaacag
     1381 tttaagagaa tgtcagaaag agtgcttgtg tcagcacttt gtgcaatcac gactgaggat
     1441 atttacaagc tggaccgtga ggcttctgat gaggtaagac agcaggtgct ggtgcgtata
     1501 gacagcaatg aagcggctgg ttttacagag cattttgacg agatcagggt gcatcagact
     1561 gaaatctcaa actatgtgaa aagagatgga aggtgcgtca tcagtataca gagtgcggtt
     1621 gagtattttt actacaagac tgcctcagga aagcttatca gtggtgataa agagtataaa
     1681 aagcagacca gatacaatat ggagcttgta tacatacagg atatagatat gctggataat
     1741 gcagggatag gcagtgcagt tggcactaca tgtccgaatt gcggcgctcc ggtcagaagt
     1801 cttggagcaa agaaatgtga atattgtggc tcatatgttg agcctgtcaa tataaaagta
     1861 tggaagctgc agagctttca tgaagttgat tacaaccata tctaaggagg nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnta ctacaaggtg agcaattcat ctatgccatc acttttcaac
     2161 ggacagtttg gggattcttt aaaggaaacc tttgaccgtg tgagatttgg tggacagaca
     2221 ccacagtcac agaaggttta ttatatcaac cttcaggaaa tcaagggaat caagtttggt
     2281 acgagaaatc ctataaacta ctatgataca ttttataatg cggagctttt ccttcgtgcc
     2341 catggtacat attccatcaa aattgtgaat ccacttcagt tttatacaga ggctattcca
     2401 aagaatatgg atcatgtgga gattactgat atcaatgagc agtatctgtc agagttttta
     2461 gaggcattac agtctgccat caaccagatg tcagcggatg gtacaagaat ttcattcgtc
     2521 acaagcaagg catctgagct tggaagatat atggcgaagt gtctcgatga aagctggaat
     2581 cagatgcgtg gtatggaggt acaaagtgtt ggtatcgcca gcctttcata tgatgagaag
     2641 tctcaggagc tcattaacac aagaaacgag ggcgcaatgc tctcagatcc atctattgca
     2701 cagggatata tggtcaaaaa tatgtcagag ggtgttaaga acgcaggcag caacagtggt
     2761 ggagctatgc agggcttcat gggtgtcggc atgggaatga gcaccatggg caatatgatg
     2821 aatggtttta cacagatggc acaaaataac cggtcagccg gaatgaatgc gccgggaaat
     2881 ggtgcagcag gtatggcagc aggagtggct tcaggtgcca cacagcagga ggatgcgtca
     2941 ggctggacat gtgagtgcgg acagaaaaac acaggtaaat tctgcagtaa ttgcggcaag
     3001 cctatgccac aggagattac agagtggaca tgtgagtgtg gacagaaaaa tacaggtaaa
     3061 ttctgcacaa actgtggcaa gccattacct gtaaacacag agtggacatg tgagtgtgga
     3121 caaaaaaata caggtaaatt ctgcagtaat tgtggaaaac caagagcata agatgttgca
     3181 aagctgtaat ctttgtgtta aaatctttga gtcaaggaca aatgtcggtg tgtgacaaac
     3241 acacaccggc cattttggag gtaactaaat ggcggagaaa tcaaactact tcgttttacg
     3301 ggcaaaggct gtaccggagg tgctgcttaa agtagttgag gcaaagagac tgctggattc
     3361 gggcaagctt gaatctgtgc aggaggcaac agatgctgtt ggcataagca gaagctcctt
     3421 ttacaaatat aaggatgata tttttccttt ttatgagggg cgtaaaggaa agacagtgaa
     3481 tcttgtcatt cagatggatg acgagcaggg catactttcc gttgtgctta agtcggtagc
     3541 cgatgcgaaa gcaaatattc ttaccataca ccaaagtgta ccggtaaatg gcattgcgtc
     3601 actcacgtta tctgtggatg tatttccgga gtcggatgat gtggatgata tggcaaagca
     3661 gattgagtcc ttaaagggca tacattacgt aaaaatttta gccagggagt aacaaaaaga
     3721 tgaagattgc agttatgggt tacggcacaa ttggatcagg tgtcgtagaa gtattagaaa
     3781 tcaacaaaga aaaaatcgca aagagagcag gagagccaat cgaggtcaag tatgtgcttg
     3841 atttaagaga gttcccgggt acacctattg agaacaaaat agttcatgat tataaggtta
     3901 ttgcagagga ccctgaaatc ggtatagttg tagagacaat gggaggcgtt gagcctgcat
     3961 ttacattcgt aaaggcaatg cttgaggcag gaaaacaggt agcaacatca aacaagaacc
     4021 ttgttgctgc aaagggtgct gagctcatca aggttgccag ggatcatgga gtgaacttcc
     4081 agtttgaggc cagtgtcggt ggtggaatcc ctattatcag acctctcaac aagtgtctca
     4141 cagcagacga gattgaggaa atcacaggta tcttaaacgg aacaaccaac tacatgctta
     4201 caaagatgac agaggaaggt gcagactttg atgaggtatt aaaggatgct caggataagg
     4261 gctatgcaga gaaggatcct acagccgaca tagagggata tgatccatgc agaaagatag
     4321 ccattctcac atcgcttgtt gcaggacagc aggtggattt cgaggatatc cactgtgagg
     4381 gtatcacaaa gattacacct gaggatatta agtatgcaaa ggcaatgcac cgtgcaatca
     4441 agcttcttgc ttcaagccgc aaggtgggtg acagctatac ctgtctcgtt gcaccatata
     4501 tgatagatga cacacatccg ctctcaggag taaatggagt gttcaacggc gtatttttac
     4561 gcggaaatgt gctcggagat gctatgttct acggcagtgg agcaggaaag cttccaacag
     4621 caagtgctgt agtggcagat gttgttgata tgacaaagca tcagaacaca aatatttata
     4681 tcgactggaa gcctgagaag ctcacacttg taagctatga tgacagtgta aacagcttct
     4741 ttgtgagaac tgactcacca aagagcgagg ttgaggctgt attcggacat gtagactaca
     4801 ttgaggatgt ggttgacggc gagtatgcat tcacaacagg cgacatgaca gagaaggatt
     4861 ttgccgacaa ggcatcaaag ataaacgtta tcaacagaat cagattataa aggacaaaaa
     4921 tatggtaaaa ggcttataca ccgctcacac cggtatggtt aatgagatga agcgcatgga
     4981 tgttcttgcg aacaacctgg ccaattccga tacctatgga tttaaaaaag agggaaccac
     5041 ctcgcattca tttaaaaatg aacttgctat aaagataaag gatacatcaa actacggact
     5101 gcacaaaaat ctgggcagca tgacatacgg agtgcatctc ggtgagacat ataccgactg
     5161 ggatgagggc agctttaagg tgactgacaa tcctacagac ttggctatcg gtggaaaagg
     5221 cttttttgcc gttgcattta ctgacaaatc
//
LOCUS       DPUF01000206           16461 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7639, whole
            genome shotgun sequence.
ACCESSION   DPUF01000206 DPUF01000000
VERSION     DPUF01000206.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 16461)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16461)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16461
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7639"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            49..123
                     /locus_tag="DHV81_07325"
     tRNA            49..123
                     /locus_tag="DHV81_07325"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:82..84,aa:Glu,seq:ctc)
     gene            complement(275..1354)
                     /locus_tag="DHV81_07330"
     CDS             complement(275..1354)
                     /locus_tag="DHV81_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HCI94263.1"
                     /translation="MRTRQLKPGMIIDQVIKDPTGRNLVVQGSAIDDYIISSLLKLGI
                     MSVYIREGNADPDDPDAILSPKAAAMVKQLRTDDRSKVTLSDSVRQRVSTGVQYIFNN
                     TNSEELTQATNQIADSLMEAINENDAIAVDISSLKTSDEYTFKHSVDVATISMILAKQ
                     MKLPDNEIHDIGVSGLLHDIGKTMIPNEILNKPGRLTDDEFNIMKKHSVYGYNMLKDR
                     KDLNNSILMGVLQHHERIDGTGYPLGFDAPKICQFAKILSVADVYDALVTKRPYKDAL
                     SQRDAVEMLMSMTNQLDIGVMRAFMSSMILYPVDSIVQLSNGEKARVVKNSEYYILRP
                     TVVGVDSGRVYNLGNDLKCASIIIL"
     gene            complement(1554..2423)
                     /locus_tag="DHV81_07335"
     CDS             complement(1554..2423)
                     /locus_tag="DHV81_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCI94264.1"
                     /translation="MTVHRNNYKSPFLHCIKCRCSALFTLFLLVTILVLSSSSNVYAT
                     ETATNYNLSGTNKSTGYEYILEDSANFIDDAVKGKLISQMKKATEYCNIAVVTTTSHP
                     YGSTESYAVNTFEGYFGTGANGVIFVIDRDLNEIYLACEGSAQRTIPNSKCNSICDNT
                     YIYATSSHDYDYFTCCMETIDQVNTVLAGGHISQPLRYISSIFIALAAGMIFCFVYAL
                     SLSKGRKAKSNELMGAAFTKVEIQNARARFMNQTRHYSPQSSGSSGGHSGGGGHSGGS
                     HSGGGHSGGGHHI"
     gene            complement(2410..4050)
                     /locus_tag="DHV81_07340"
     CDS             complement(2410..4050)
                     /locus_tag="DHV81_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94265.1"
                     /translation="MFACKNCGGNVKFDIKSGQLACEYCHSLFDPYAYEDKTSDAEVQ
                     KDFDATIFTCPQCGGEILSTDDTAAGFCSFCGASTVLYSRMQKEHKPAYIIPFAKSKD
                     DCKQAYMSLMKKAIFAPKELKDPKFIDGFRGIYMPYWTYYVTQKAPISLPAKRSHRSG
                     DYIITDYYRLEGDLDAYYKGLSYDASSSFDDSISEKLAPYDVKKMKRFTPAFLSGFYA
                     DTADLPSTVYASDAMDAACTNTVSEISKEPAFTGLSVDSDSAALSPLSLGTTVKETDY
                     SMFPVWFLSYRNKDRVAYATVNGQTGKVVADLPISVGKFLLGSLIAAIPVYILLCLLT
                     VLTPGMTLTIVGVLAIIANICYSQELTMIAVKEAGTEDKGRIAKEQPEALGAINNRRR
                     LKAAKKATKTIKKKTNTSFVAYFILFIFVIQFVPALFAIIAGIGGSFSNADGSLILFV
                     ILTIISFIFSIRAFSSFDRMPGHKGVAGLIFGMVSMLIGDAVLLFQPVLDAWYYGAAF
                     IIIASVLITLINVIRAFNVLTTRKLPQFATHKGGDDRA"
     gene            complement(4037..5536)
                     /locus_tag="DHV81_07345"
     CDS             complement(4037..5536)
                     /locus_tag="DHV81_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCI94266.1"
                     /translation="MNTSVIRYLLGYILKLEGFLLLLPCIVAGIYYEKSGIYFLIVAL
                     ISIAIGFIFSAKKPRDFTFYLKEGCATTALGWVVLSIFGCLPFYISGEIPSFTDALFE
                     TISGFTTTGASILPEVESLSYCMNFWRCFTHWIGGMGVLVFLLAIIPLSGGSNINLMR
                     AESPGPSVGKLVPKMKHTARLLYIIYFGLTTMEIIFLLFGKMPLYDAICTSLGTAGTG
                     GFGIKNDSFCGYNVYLQWVVTIFMILFGVNFNAYYLLFFGHIRDALKVEEVRYYFGII
                     IASVVVISVNIAHMCTGVFDAVTKAAFQVGSIITTTGFSSTDFDRWPELSKTLLVLLM
                     FIGACAGSTGGGIKVSRIVIAVKTILKELNGYIHPKSVKKLTFEHKPVDHDVIRSINV
                     YFMTYAVIFVVSMLLVSVENYDFTTNFTAVAATFNNIGPGLSLVGPTCNFGFFNNFSK
                     YVLMFDMLAGRLELFPLLILFHPSIWKELFIQADKKVKGNRKEKQNVRM"
     gene            complement(5549..6937)
                     /locus_tag="DHV81_07350"
     CDS             complement(5549..6937)
                     /locus_tag="DHV81_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCI94267.1"
                     /translation="MFKKGVVPSQGLRIIIVGCGKVGRTLVEQLGKEGHDITIVDKNR
                     ERISQVSNIYDVMGIAGNGASYKILQEAGIDDADLIIAVTGSDELNLLCCTIATQVGN
                     CAAIARVRTPEYSQEAKYLRDKLGLALIINPELEAAIEMAHILHLPSSLEVTNFAHGQ
                     AQLIKYEIPEGSILDGTKLMDLGTRHHANILIGAVERDDEVTIPSGDFVLRKGDKLSF
                     VGERRHTKEFFSHIGVNTHSVKNTLIIGGGKAAYYLAKQLISRGIKVKIIENSFERCE
                     ELSILLPDAVIINGDGTEQALLKEEGIETCQSFVPLTGIDEENIMLTLYAKQVSNAKV
                     ITKLNRITFTNVINSLDLGSVISPKYITSEAIIAYVRAKKNSMNCNIETLYHMYDSRV
                     EAIEFFVSENSNVTDIPLRDLKLKKHLLICFINRNGKIIIPTGNDCIQKNDTVMIITT
                     HTGFTNLQDIME"
     gene            7201..7971
                     /locus_tag="DHV81_07355"
     CDS             7201..7971
                     /locus_tag="DHV81_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCI94268.1"
                     /translation="MTGFLKANYHTHTYRCQHAYGSEREYIEAAIRMGIAELGFSDHV
                     PCPFKDGYVSGIRMTMEQAPEYVYAIRELGKEYASDIKLYVGFEAEYIPEFFKEQKAM
                     FDRLGCDYMIMGQHFMKSEQTGPYTGTPTDDESRIREYVDTVIEGMKTGMYSYLAHPD
                     LINYQGLDSVYEWEMTRMCKALKELNIPLEINMLGISAGKHYPTERFWCIPHDIGNKV
                     ILGLDAHTVDNICDVESYEKALDIVRKYDLNLIDRIEI"
     gene            8011..8958
                     /locus_tag="DHV81_07360"
     CDS             8011..8958
                     /locus_tag="DHV81_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCI94269.1"
                     /translation="MIITEEKYNEELTRYVLPFIRLNFEDGYMKSADGTRIHYGYVTT
                     PVAKAAIVISHGFTECMPKYYEMIYYFAKAGYSVYMVEHRGHGFSDRSVSDISMVTVN
                     SFDDYVSDLDMFIREIVMKREGRRPLYLYGHSMGGAIAALYLEKHPEVFTKAVLSSPM
                     IEMLYGNFSHFAVEAILFVASVLNWNDKYLPSQTPYTDEYDFESSCCLSKARYDYIYK
                     CKVEEERYRTNGATYRWCRAGRKASKYIKKHAQEIKIPVLLCQAGKDYLVSNAAEDEF
                     IAKLPQGIKKVYPDSKHEIFNADDDTLEKFYSDILDFWA"
     gene            9618..10103
                     /locus_tag="DHV81_07365"
     CDS             9618..10103
                     /locus_tag="DHV81_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCI94270.1"
                     /translation="MKRVLVLSDSHGNVGNMIRAVKREEPDMILHLGDCVVDADALRR
                     EFPHITMVNVPGNCDFSRGDTERLIDIDGYKVLMCHGHTYGVKMSYMHLELHAKEVGA
                     DLALFGHTHKLFYDKHNGLAMMNPGSIGAPLWGCMPSYGIITFDKEHDVMKLDVDYIE
                     Y"
     gene            10178..11497
                     /locus_tag="DHV81_07370"
     CDS             10178..11497
                     /locus_tag="DHV81_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCI94271.1"
                     /translation="MVKVVKFGGSSLASAEQFTKVGDIIRSDESRKYVVPSAPGKRNS
                     KDTKVTDMLYACYDLAENDQDFKVMLRKIKDRYDSIINGLHLKLALDEDFKIIAENFK
                     AKAGADYAASRGEYLNGIIMANYLGYEFIDSATVIFFDENGNFDAEKTDKVLSKKLEQ
                     TEKAVIPGFYGAGPDGKVVTFSRGGSDITGSIVSKACHASMYENWTDVSGCLVADPRI
                     IDNPQPIKVVTYRELRELSYMGASVLHEDAVFPVRKAGIPINIRNTNDPEAPGTLIVE
                     STCQKPDYTITGIAGKKGFVAVNIDKDKMNSEVGFCRKALQAFEENGISIEHMPSGID
                     TMTVFVHQAEFEGKEQQVISSIRRLAHPDIIDLESDLALIAVVGRGMKSNRGTAGRIF
                     SALAHANINVRMIDQGSSELNIIIGVSNDDFDKAIKAIYDIFVVTQL"
     gene            11723..12529
                     /locus_tag="DHV81_07375"
     CDS             11723..12529
                     /locus_tag="DHV81_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit A"
                     /protein_id="HCI94272.1"
                     /translation="MQKDTIIYVTDQRQKYLANMLGGEQTDCRNSGEIDYERVGNIVF
                     PTPFSKLKLINSDIEKLKHNIIINNIAVWGGMMPERFPGIDRGCDFMRDETVIEQNAI
                     VTAEATASIAIQKSIHGIYGSKVLVSGFGKCGKAIARVFSAMGAHVMVMARRKQARHE
                     AAELGYETVDFNDPAKACFETLFLINTVPDRVIDESLIMILQKDAIIIDIASKPGGCD
                     FEACKRYQINCTHALGLPGIYCPKTGAKILYDCMKRKGMTEGLWLFRIAP"
     gene            12505..13083
                     /locus_tag="DHV81_07380"
     CDS             12505..13083
                     /locus_tag="DHV81_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HCI94273.1"
                     /translation="MVISDCTVGMGITGSFCSFEKTKDVAKRLVDSFTHVTPVYSYNA
                     QMMNTRFGKAGDFMHTISEITGDEGIRTLQEAERVGPGKLFDVMVIYPCTGNTAAKLA
                     NGIVDTPVLLAAKAHLRNGRPLVIGISTNDALGINFKNIGKLMNMKNIYFVPFGQDDC
                     VKKPNSLVCDGKQVVDTINEVVAGRQIQPVIL"
     gene            13584..13844
                     /locus_tag="DHV81_07385"
     CDS             13584..13844
                     /locus_tag="DHV81_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HCI94274.1"
                     /translation="MSYIYKTKGTCSTQIELDLDGDVVHNVKFTGGCNGNLKAIPKLV
                     EGLTVAQVEEKIGGIKCGFKNTSCGDQLAKACREAYEAQKNN"
     gene            complement(13841..14023)
                     /locus_tag="DHV81_07390"
     CDS             complement(13841..14023)
                     /locus_tag="DHV81_07390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94275.1"
                     /translation="MGGCEVRGAHSIFLIKNMNFFILNTLCTSRIHFVVREAWWNYVQ
                     FKKLSTRESFLELPIF"
     gene            14183..>16461
                     /locus_tag="DHV81_07395"
     CDS             14183..>16461
                     /locus_tag="DHV81_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCI94276.1"
                     /translation="MSAFSKIFGSHSERELKRIYPIADKVESYKEAMGKLSDEELKDK
                     TREFKKRLEDGATLDDILPEAFATVREAAKRVLGMEHYRVQIIGGIILHQGRIAEMRT
                     GEGKTLVSTLPAYLNALEGKGVCIVTVNDYLAKRDAEWMGQVHEFLGLKVGVVLGGMD
                     NDERREAYGCDITYITNNELGFDYLRDNMVIYKEQLVQRGLHYAIIDEVDSVLIDEAR
                     TPLIISGQSGKSTRLYEACDILATQMKRGEDVPEYSKMDAIMGIVQDETGDFIVNEKD
                     KVVNLTQDGVKKVEQFFHIENLADPENLEIQHNIILALRAHNLMFKDQDYVVKDDEVL
                     IVDEFTGRIMPGRRYSDGLHQAIEAKEHVKVKRESKNLATITFQNFFNKFDKKAGMTG
                     TALTEEKEFRDIYGMDVVEIPTNKPVARIDLQDAVYATKKEKFKAVVDAVKEAHEKGQ
                     PVLVGTITIETSELLSGMLKREGIPHTVLNAKFHEKEAEIVALAGQHGAVTIATNMAG
                     RGTDIKLDDEAKAAGGLKIIGTERHESRRIDNQLRGRAGRQGDPGESQFFISLEDDLM
                     RLFGSERLMGVFNALGVPEGEQIQHKMLTSAIEKAQEKIEANNFGIRKNLLEYDQVDN
                     DQREIIYSERMKVLNGDNMRDAILKMVQEQVEKSVDRCISEEIDRADWDLVELNELVL
                     PVIPLDPITKEDIESIKNAKQLKQYLKEKAVMLYEQKETEFPEPEQFREIERVVLLRV
                     IDRKWMDHIDDMAQLRQGIG"
BASE COUNT         4842 a         3242 c         3961 g         4416 t
ORIGIN      
        1 ttcaaaaaaa gcttgcatta tccgattgac catggtatta taatacacgg ttcgttggtc
       61 aagcggttaa gacgccgccc tctcacggcg gaaacacggg ttcgattccc gtacggactg
      121 ctaaaacaaa aagctctcag tacacagtgc tgagggcttt ttatttttgc aagcaattaa
      181 taactataaa accattgaga acaaaaagct gatgcctgca tgtaagcatg acgcatcagc
      241 ttttttattc ttaaggtggc tcaaatcatt atgtctaaag tataataata cttgcgcact
      301 tcaaatcatt gcccagattg tatactctgc cggaatcgac accgacaaca gttggtcgga
      361 gaatgtagta ctcactgttt tttacaactc ttgccttttc accattgcta agctgtacaa
      421 ttgaatcaac aggatataga atcatactgc tcatgaaagc acgcatgaca cctatatcaa
      481 gctggtttgt catggacata agcatctcaa ctgcatcacg ctgtgataag gcatccttgt
      541 acggacgctt tgtgacaagc gcatcataga catctgcgac agacaaaatc tttgcaaact
      601 gacagatttt tggtgcatca aagccgagtg gatatccggt tccgtcaatg cgttcgtggt
      661 gctgcagcac tcccatcagt atagagttgt ttaaatcctt tcggtctttt agcatattgt
      721 agccgtacac ggaatgtttt ttcatgatat taaactcatc atccgtgagc ctgccaggct
      781 tgtttagaat ttcattgggg atcattgtct tgcctatatc gtgcagcagg ccggatacac
      841 ctatgtcgtg gatttcgttg tcaggcagct tcatctgctt ggcaagaatc attgaaatag
      901 tagccacatc cacactgtgc ttgaaggtgt actcatcgct tgttttgagg gaactgatat
      961 cgacagctat ggcatcgttt tcattgatgg cctccatgag cgagtctgca atctggtttg
     1021 ttgcctgtgt tagctcctcg gagttagtgt tgttgaagat gtactggaca ccagttgata
     1081 cacgttgcct gacactgtcg cttaaggtga ctttggaacg gtcgtccgta cgcagctgtt
     1141 tgaccatggc ggcagccttt ggagacagta tggcatccgg atcgtcagga tcagcatttc
     1201 cctcgcggat gtatactgac attataccga gcttaagcag cgaagagatg atataatcat
     1261 cgatagctga gccctgtacc accaggttac ggccggtagg atccttgatg acctgatcaa
     1321 taatcatgcc gggtttcaat tgtcgtgttc tcatatatct gcgtttaatc atttttgcac
     1381 caaaactaaa taagtctact ctaaaagaca atgtcaaaat actacgaata ttatatagta
     1441 tatttgtact aaaaacaatc acaaattgag atttttttag tgtttttttg taataaaaca
     1501 accccggcat ttgccggggc tgatgatggt attgtattta tcaaaatcag aggttagatg
     1561 tggtgaccgc caccactgtg accgccacca ctgtgactgc cgccactatg acctccacca
     1621 ccgctgtgac cgccacttga gcctgagctt tgtggactgt agtgtcttgt ctggttcata
     1681 aagcgtgcgc gtgcattttg aatctctacc tttgtaaatg ctgcacccat gagctcgttg
     1741 ctcttagcct tgcggccctt tgacagggat aatgcatata caaagcaaaa aatcatgccg
     1801 gcagcgagag cgataaagat actggaaatg tatctgagag gctgtgatat atgtccgcct
     1861 gcaaggactg tgttgacctg gtctattgtt tccatacagc aggtgaaata gtcgtaatca
     1921 tgacttgagg tggcatatat ataagtgttg tcacagatgg agttacattt tgagtttgga
     1981 atggttctct gcgcgcttcc ctcacaagcc agataaatct cgtttaaatc tctgtctatt
     2041 acaaaaatga caccgtttgc accggttccg aaatagcctt caaaggtatt gactgcatag
     2101 ctttcagtgc ttccgtaagg atgtgaggta gtggtgacaa cagctatgtt gcagtattcg
     2161 gttgcctttt tcatctgtga gatgagcttg ccttttacgg catcgtcaat gaaattggca
     2221 gagtcctcaa ggatgtactc gtagccggta gacttgtttg tgccgcttag attgtaattt
     2281 gtggctgtct cagtggcata tacatttgat gagctgctca aaacaaggat tgtaacaagc
     2341 aaaaataatg taaacaaagc tgaacacctg cattttatac aatgcaaaaa aggactttta
     2401 taattatttc tatgcacggt catcacctcc cttatgtgtg gcaaactgtg gcagcttgcg
     2461 cgttgtaagc acattgaatg cgcggatgac attaatcagt gtgataagca ctgaggcgat
     2521 gatgataaaa gctgcgccat agtaccatgc atcgagcacc ggctggaaca aaagaacagc
     2581 atcgccaata agcatggata ccatgccaaa gatgagtccg gcaactccct tgtgtccggg
     2641 catcctgtca aagcttgaga aggctctgat tgaaaaaata aatgatatga tggtcaaaat
     2701 aacaaataaa atcaggctgc cgtctgcatt gctaaagctg cctccgatac ctgcaatgat
     2761 tgcaaacaga gcaggaacaa attgaatgac aaatataaaa agtataaagt aagctacaaa
     2821 cgatgtgttt gtttttttct ttatggtttt agtggccttt tttgcagcct taaggcgtct
     2881 gcggttgttg attgcaccaa gtgcctccgg ctgttccttt gcgatacggc ccttatcctc
     2941 ggtaccggct tcctttacag caatcatggt cagctcctgt gagtaacaga tatttgcgat
     3001 aatagcaagc acaccgacta tggtgagagt cattccggga gtgagaaccg tcagcagaca
     3061 taataaaatg tataccggaa ttgctgcaat gagtgagcct agcaggaatt taccgacaga
     3121 tataggcaga tctgcaacaa ccttgccggt ctggccgttt accgtggcat atgccacacg
     3181 atccttattg cggtacgaca aaaaccatac cggaaacatt gagtaatctg tctcttttac
     3241 agtagtgcca agacttaaag gagaaagagc agcggagtca ctgtcgactg acaagccggt
     3301 aaaggctggc tctttactta tttcgcttac tgtgtttgtg caggcagcat ccattgcgtc
     3361 acttgcgtaa accgttgatg gtaaatcagc tgtatcagcg taaaaaccac ttaagaatgc
     3421 cggtgtaaag cgcttcattt tcttcacatc atatggcgca agtttttcgc tgatgctgtc
     3481 atcaaatgat gaagatgcat cgtaggacag tcccttgtag taggcgtcga gatctccctc
     3541 gagcctgtaa tagtctgtaa tgatgtaatc tcctgaacgg tgggatcttt tcgccggaag
     3601 tgaaatagga gccttctggg ttacataata tgtccagtaa ggcatgtaga tacctctgaa
     3661 tccgtcgata aacttaggat ccttaagctc ctttggcgcg aaaatagcct ttttcatgag
     3721 gctcatgtag gcctgcttac agtcatcctt actctttgca aatggtatga tataagccgg
     3781 cttgtgctcc ttttgcatgc ggctgtagag cacagttgat gcaccgcaga agctgcagaa
     3841 gcctgctgcg gtatcgtctg tgctcaggat ttctcctccg cactgagggc atgtgaaaat
     3901 ggttgcgtcg aagtccttct ggacctcggc gtcagaggtt ttgtcctcgt acgcgtaagg
     3961 gtcgaaaagt gaatgacagt attcacaggc taactggcct gatttgatgt caaacttcac
     4021 atttccaccg caatttttac atgcgaacat tttgtttctc ctttctattc ccttttactt
     4081 ttttatctgc ttgaataaat aattccttcc agattgacgg atggaagagg atgagcagtg
     4141 gaaacagctc gagtcttccg gcaagcatgt caaacatcag tacatatttg gaaaaattat
     4201 taaaaaagcc aaaattgcat gtcggaccga ccagagaaag acccggtccg atattgttaa
     4261 atgtagctgc gacagccgtg aaattggtag tgaaatcata gttttcaacg gacacaagca
     4321 gcattgaaac aacaaaaatg acggcatacg tcatgaaata aacatttatt gacctgataa
     4381 catcatggtc tacaggcttg tgctcaaatg tgagcttttt cacactcttt gggtggatgt
     4441 agccatttaa ctccttaagt attgtcttta cagcgatgac aatacgagat accttgatac
     4501 cgccgccggt gcttccggca catgcgccaa tgaacatcaa aagcacaagc agtgtctttg
     4561 aaagctcagg ccatctgtcg aaatcggtgc tcgagaatcc ggttgtcgtg atgattgagc
     4621 caacctgaaa cgctgccttt gtgactgcgt caaacacacc ggtacacata tgcgcgatat
     4681 tcacgcttat gactacaacg gaggctatta tgatgccgaa atagtatcta acctcctcca
     4741 ccttaagtgc gtcacggatg tgaccgaaga aaagcaggta gtaagcgttg aaattgacac
     4801 cgaaaagtat cataaatatg gtaacaaccc attgcagata aacattgtag ccgcaaaagc
     4861 tgtcgttttt gataccaaag cctcctgttc cggctgtgcc aagagaggta cagatggcat
     4921 cgtaaagagg cattttccca aaaagcagga aaataatctc catagtcgta agaccaaagt
     4981 atattatgta taaaagcctg gcggtatgct tcatctttgg aacgagcttt ccaactgaag
     5041 gcccggggct ttctgcgcgc atcaggttga tattgctgcc gcctgaaagc gggatgattg
     5101 ccagaagaaa aacaagcaca cccataccgc caatccagtg cgtaaagcag cgccagaaat
     5161 tcatgcaata tgagagtgat tctacctcag gcaggatgct cgctcctgtc gtggtgaagc
     5221 cggagatggt ctcaaaaaga gcatctgtga agcttggaat ctctcctgat atgtaaaacg
     5281 gcagacagcc gaatatactc agtacgaccc agcccagtgc ggttgttgca cagccctcct
     5341 tcaggtaaaa ggtaaaatcc ctcggttttt tagccgaaaa aataaaaccg atagctatgc
     5401 ttatgagtgc aacaatcaaa aagtagatgc cgcttttttc ataataaata ccggcaacaa
     5461 tacatggcag aagcagcaaa aagccttcaa gctttaaaat atatcccaaa agatatcgaa
     5521 ttactgaagt gttcatatca ttcccccact attccataat atcctgaaga tttgtaaagc
     5581 cggtatgggt tgttataatc atgacagtat catttttttg tatgcagtca ttacctgtag
     5641 gtataataat tttgccgttt cggttgatga agcagatgag cagatgcttt ttgagcttta
     5701 ggtctcgaag cggtatgtct gtcacatttg agttttctga tacaaagaac tcaattgctt
     5761 caactcttga gtcgtacatg tggtagagag tctctatatt gcagttcata gagtttttct
     5821 ttgcacggac ataggcaatt atggcttctg aggtgatgta ttttggcgag attacactgc
     5881 caaggtccag gctgtttatg acattggtaa aagttatacg attaagcttt gttatgacct
     5941 ttgcattgga aacctgcttg gcatagagcg taagcatgat gttttcctcg tctataccgg
     6001 taagaggtac aaatgactgg caggtctcta tgccctcttc ctttaagagt gcctgttctg
     6061 ttccgtcacc gtttatgatg acagcatccg gaagcagtat ggaaagctcc tcacagcgct
     6121 cgaagctatt ttcgattatt ttgaccttta tgcctcgtga tatgagctgc tttgccaggt
     6181 aatatgcggc tttgccgcct ccgataatca gtgtattttt tacgctgtgt gtattgactc
     6241 cgatgtggct gaaaaattcc tttgtatgtc tgcgttcgcc gacaaatgac agcttgtcgc
     6301 ccttgcggag cacaaaatca cctgatggga tagtgacctc atcatctctc tcgactgcac
     6361 cgataagtat gttggcatgg tgtctggtgc ccagatccat gagctttgtt ccgtcgagga
     6421 tgctgccctc gggaatctca tacttgatga gctgtgcctg accgtgagca aagtttgtta
     6481 cctcaaggct ggacggcaga tgaagaatat gagccatctc aatagctgcc tcaagctcag
     6541 ggttgataat gagtgcaaga ccaagcttgt cgcgcagata tttggcctcc tggctgtatt
     6601 caggcgttct gactctggct atagcagcac agttgccgac ctgtgtggca atggtacagc
     6661 atagcagatt cagctcatca gagcctgtga ctgcgatgat taagtcggca tcatcaatac
     6721 ccgcttcctg cagtatcttg tagctggcac cgtttcctgc aatacccatt acatcataga
     6781 tattggatac ctgggaaatc ctctccctgt ttttgtcgac aattgttatg tcgtgcccct
     6841 ccttgccaag ctgctcaacg agagtacggc ctaccttgcc gcatccgacg atgataattc
     6901 tcagtccctg ggagggaacc actccttttt tgaacattat ttcctccgta tataaaaata
     6961 taatgatatg aggatcaaaa ggtattttgc ccctcattta tgattgtgtg gtggatagaa
     7021 ttattatgac tatcatatat caatggatta taacataacg catatatatt tcatagagaa
     7081 aaaagtccca aaaatatgag attgtaataa agtgcaaaaa gtgataaaat tgattttaat
     7141 tgtagtattt gaatagctgt ttgaacagca ataagagata gatttttgga gctttaagaa
     7201 atgacgggat ttttaaaggc aaactaccat acgcacacct ataggtgcca gcatgcatat
     7261 ggcagcgagc gcgaatatat agaggcggct atcagaatgg gtatcgcaga gctaggtttt
     7321 tcagaccatg tgccgtgccc ctttaaggat ggctatgtgt cgggcatccg gatgacgatg
     7381 gagcaggcac cggagtatgt gtatgccatc agggagcttg ggaaggagta tgcatccgac
     7441 ataaagctct acgttggctt tgaggcggag tacataccgg agtttttcaa ggagcagaag
     7501 gctatgtttg acaggcttgg ctgtgattac atgataatgg gccagcattt tatgaaatca
     7561 gagcagacag gtccgtatac aggcacaccg acagatgatg agagtcgtat acgcgagtat
     7621 gtggatactg ttatagaggg catgaaaaca ggcatgtaca gctatttagc acatcctgat
     7681 ttaataaatt atcagggact tgattctgtc tacgaatggg agatgacgag gatgtgcaag
     7741 gcacttaagg agcttaacat accgcttgag ataaacatgc ttggtatcag tgcaggaaag
     7801 cactatccga cagagcgctt ctggtgtata ccgcatgaca tcggcaacaa agtgatactt
     7861 gggcttgatg cacatacagt ggacaatata tgtgatgtgg aaagctatga aaaggcactt
     7921 gatatagtca gaaaatatga cctgaatctt attgaccgga ttgagatata gttacttaca
     7981 aaatatcaaa taccaaaagt gaggaatgct atgattatta cagaagaaaa atataatgag
     8041 gaattaacaa gatatgtttt gccttttatc aggcttaatt ttgaagatgg ctatatgaag
     8101 agtgcagatg gcacacgcat acattacggc tatgtgacga cacctgtagc aaaggctgcc
     8161 atagtcatat cacatggatt tacagagtgt atgcctaaat attatgagat gatttactat
     8221 ttcgcaaagg ccggatactc agtatatatg gtggagcatc gaggacacgg cttttcggac
     8281 agaagtgtat ccgatataag catggtcact gtaaacagct ttgacgatta tgtgagtgac
     8341 cttgatatgt ttatccgcga gattgtgatg aagcgtgagg ggcgcagacc gctttacttg
     8401 tacggtcact ctatgggagg cgcgatagct gccctgtatc ttgagaagca tcctgaggta
     8461 tttacaaagg cagtactttc aagccctatg atagagatgc tttatggtaa tttttcacat
     8521 tttgccgtgg aggcgatact gtttgtcgca agtgtgctta actggaacga taaatatctg
     8581 ccgagccaga caccgtatac ggatgagtat gattttgaat cgagctgttg tctgtcaaag
     8641 gcgcgctatg actacatata caagtgcaag gtggaggagg aacgctatcg tacaaatggc
     8701 gccacctaca gatggtgcag ggccgggcgc aaggcatcca aatatataaa gaagcatgca
     8761 caggaaatta aaataccggt gctgttatgc caggcgggaa aggattatct ggtttcaaat
     8821 gccgctgagg atgaatttat tgcgaaactg cctcagggca ttaagaaggt ctatcctgat
     8881 tcaaagcacg agatatttaa tgcggatgat gataccttgg agaagtttta ctcagatatc
     8941 cttgattttt gggcttgaac atgaaaatga aattcgtcaa gtgaaaaatg tgacataaaa
     9001 aatcatttgt tttgttagaa atgacatatg gatttaaaat acaatatata gtataatcac
     9061 aactgttgcg gacaagatgt tagtatttaa gccgatggta agagtacgta aagcatagcc
     9121 cgtacagaga aaaataataa aatatatcaa tattgtaaca ggtaaatata ctatcaaaca
     9181 aatgtgagga gaaagaaaat gtcagacgaa gttttagacg atgtaatgat taagggttca
     9241 tgtaagggaa aagcagatgc tattgtaaat tgccagtgca atatcgagag tgcaccggtc
     9301 agatgcgagc aggcagaaag atgtatgttc tattcttatt ataagtgcag caagcagtgg
     9361 aagaggcttg catagttaaa attaaagatt aaaagctccg ggctgacaat gaagtcggca
     9421 tggagctttt ttgcgtatat aaagaaaaaa atattttaca cgcggaaaac ttatgataat
     9481 atataaaaaa ggtaactatt cagaaccatt gagaacaaaa aaagctgatg cgtcatgctc
     9541 gcatgtaagc ggcagatttt cttgttctca aggtggctct ggatggatac gtaaaacaag
     9601 atataggaga gacgcgcatg aagagagttt tggtactgag tgattcacat ggtaatgtgg
     9661 gaaatatgat tcgggcagtc aaaagagagg agcctgatat gatacttcat ctgggagact
     9721 gcgttgtgga tgcggatgcc cttaggagag agtttcctca tataaccatg gtcaatgtgc
     9781 cgggaaactg tgatttttca cggggagata cagagagact tattgatatc gacggctata
     9841 aggtactcat gtgccatgga catacatatg gagtaaagat gtcatatatg catctggagc
     9901 ttcatgcgaa ggaagtcggg gcagacctgg cgctctttgg acatacacac aagctgtttt
     9961 atgataagca caatggtctt gcgatgatga atccgggaag catcggtgca ccgctgtggg
    10021 gctgcatgcc atcatacggt atcatcacat ttgacaaaga acacgatgtt atgaagcttg
    10081 atgttgatta tattgagtat tgacgaatga tttatcaaag cggtataata ttggctaata
    10141 cagttattta tataggaagc agaggaaaaa cagacaaatg gtaaaggtag taaagtttgg
    10201 cggcagctcg ctcgccagtg cagagcagtt cacaaaggtt ggcgacatca taagaagcga
    10261 tgagtcaaga aaatatgttg tgccgagtgc accgggaaag agaaattcca aagacacaaa
    10321 ggtaacagat atgctttatg catgctatga tctggctgag aatgatcagg atttcaaggt
    10381 tatgctcaga aaaatcaagg acagatatga ttcaatcata aatggccttc atttaaagct
    10441 ggcgcttgat gaggatttca agattatcgc tgagaatttc aaggcaaagg caggtgcaga
    10501 ttatgcagcc tctcgtggtg agtacttaaa cggaatcatc atggcaaact atctcggata
    10561 tgagtttata gattcggcaa cagtcatctt ttttgatgag aatggcaatt ttgacgcgga
    10621 aaagacagac aaggtgctct caaagaagct tgagcagaca gaaaaggctg ttattccggg
    10681 cttttacgga gcaggacctg atggaaaggt agtcaccttc tcaagaggtg gttcggatat
    10741 cactggttcc atcgtgtcaa aggcatgtca tgccagcatg tacgagaact ggacagatgt
    10801 ttcaggctgt ctcgtagctg atcctagaat tatcgacaat ccacagccaa tcaaggttgt
    10861 tacttataga gagctcagag agctttcata tatgggagca tcagtgctcc acgaggatgc
    10921 tgttttccct gtaagaaagg caggcatccc aatcaatatc agaaatacaa acgacccgga
    10981 agcacctggt actctcatcg ttgagagcac atgccaaaag cctgattata ccatcacagg
    11041 tatcgccgga aagaagggct ttgttgcggt aaatatcgac aaggacaaga tgaactcaga
    11101 ggtgggcttc tgtagaaagg ctctccaggc gtttgaggag aacggtatct ctatagagca
    11161 tatgccatca ggtatcgata ctatgacagt attcgtacat caggcagagt ttgagggcaa
    11221 ggagcagcag gttatcagct caatcagaag acttgctcat cctgatatca ttgacctcga
    11281 gtcagacctc gcgcttatcg ctgttgttgg ccgtggcatg aagtcaaacc gtggtacagc
    11341 cggcaggatt ttctctgcac ttgcacatgc caatatcaat gtgcgcatga ttgatcaggg
    11401 ttcatcagaa ctcaatatca tcatcggtgt aagcaatgat gattttgata aggcaataaa
    11461 agccatctac gatatttttg ttgtcacaca gctgtaataa gctacggaga gattatccgt
    11521 tggaaccaga ataggataat ttaaaaatta aatgtagaat ttataaaaca tacagggctt
    11581 ggcagaatat atgccaggct ctgtattttt tgaataggta aaactttgaa aaagctttat
    11641 gaggaagctt taagcgagaa aacaaaatac cctcacataa gctggaaatg cgcatatatt
    11701 atagcgaaag gaagaagtta ttatgcaaaa agacactata atatatgtta cggatcagag
    11761 gcaaaagtac ctcgctaata tgctgggagg agaacagaca gattgcagaa atagcggcga
    11821 gattgattat gagcgtgttg ggaatattgt gtttcctaca ccgttttcaa agcttaagct
    11881 tataaatagt gatatagaaa aattaaaaca taatataata attaacaata ttgcagtgtg
    11941 gggcggcatg atgccggagc gctttcccgg tattgacagg ggctgtgact ttatgagaga
    12001 tgaaactgtc atagagcaga atgccattgt tacggccgag gccactgcgt ccatagcaat
    12061 acaaaaaagc atacatggta tatacggctc aaaggtactt gtgagcgggt ttggaaaatg
    12121 cggaaaggca attgcccgcg ttttttctgc tatgggagcc catgtcatgg tgatggcaag
    12181 gagaaaacag gcaaggcatg aggccgctga actgggatat gaaacggtgg attttaatga
    12241 tcctgccaag gcctgttttg aaacactgtt tctcataaac acagtaccgg atagggttat
    12301 agatgaaagt ttaatcatga ttttgcaaaa agatgcgata attatagaca ttgcatctaa
    12361 gcccggcggc tgtgattttg aggcttgtaa gaggtatcag ataaactgta cgcatgcgct
    12421 cggccttccg ggcatatact gtccgaaaac cggtgcaaaa atactttatg actgtatgaa
    12481 aagaaaaggt atgacggagg gcttatggtt atttcggatt gcaccgtagg aatgggaatt
    12541 acaggctcct tctgtagttt tgaaaagact aaggatgttg caaagcggct tgtggatagc
    12601 ttcacacatg tgacacctgt atattcctat aatgcacaga tgatgaacac acgctttgga
    12661 aaggcggggg actttatgca tactatctcc gaaatcacag gggatgaggg catcaggaca
    12721 ctgcaggagg cagagcgtgt cggcccagga aagctgtttg acgtgatggt gatttatcca
    12781 tgcaccggca acacagcggc aaagcttgca aacggcatag tggatacacc ggtccttctc
    12841 gcggcaaagg ctcatctgag aaacggcaga ccgctcgtta tcgggatttc cacaaatgat
    12901 gcgctcggca tcaactttaa gaatatcgga aagctgatga atatgaaaaa tatttatttt
    12961 gtgccgtttg ggcaggatga ctgtgtgaag aaaccgaatt cactcgtgtg tgatggaaaa
    13021 caggttgtcg acacaataaa tgaagtggtg gccggacgac agatccagcc ggtaatcctc
    13081 tagagtggga atattacttt attgagatta tagtacggga cgattcacgc gggcaccgcg
    13141 gcgggctgca tacactccgg tgtccgctac gctgcgcagc caagatactg tgcaaattat
    13201 gctttgtagg ttttcatagg atgacgaaaa cgtcgaaact cgccatccat ggctcgattt
    13261 ctccttgcct cggcgtccat gcctcggcat ttctgcgtag catgtgcata attcgacagt
    13321 atctaagccg cttcgctatt cgcggactcc ggagtgtatg cagcccgccg cggtgtccgc
    13381 gcgaatcgtc ccttacgtta tggcgtaaat agtaaataaa taagatttat gaatctgaat
    13441 cgttgccggt cacactgtta gttgtgaaca gtaacttctg aattgaaaaa ctgagttgga
    13501 aaatgtaaaa aaatagagga aagatgtaaa aatatatgtt ataatcgtgt atacacaaaa
    13561 atgaaaacac aggaggataa aatatgtcat atatttacaa aacaaaagga acctgttcaa
    13621 cacagattga gcttgacctc gatggcgatg tagtacataa cgtaaagttt acagggggct
    13681 gtaacggaaa tcttaaggct atcccaaagc tcgttgaagg actcacagtt gcccaggtag
    13741 aggaaaaaat cggtggaatt aaatgtggat ttaagaatac atcctgcggc gaccagctcg
    13801 caaaagcatg ccgtgaagca tatgaagccc agaagaataa ctaaaaaata ggtaactcca
    13861 aaaagctctc ccttgtggag agctttttga attgaacata attccaccat gcttcgcgca
    13921 caacaaagtg gatccgggaa gtacacagtg tgttcagaat aaaaaagttc atatttttta
    13981 tgaggaatat actgtgtgct ccccggacct cacagcctcc catgcgcgaa gtctccccat
    14041 taaagctcgg cacttccaca aatattttta taaaatgttt acaaaacatt catcccttta
    14101 ggttgtgcaa tcatcctcaa agtgttacaa taacactatt gtgagcctgt acaagggcaa
    14161 tttgaagtta taggagaaaa atatgagcgc attttcaaaa atctttggtt ctcacagcga
    14221 gagagagctt aagagaattt acccgattgc tgacaaggtt gagtcatata aggaagcaat
    14281 gggaaagctt tccgatgaag aattaaagga taagacaagg gagtttaaaa agcgtctgga
    14341 ggacggagca acacttgatg atattcttcc ggaggcattt gctacagtaa gagaggctgc
    14401 taagcgtgtg cttggcatgg aacattatag agtacagata atcggtggta ttattttgca
    14461 tcagggacgt attgcagaga tgcgaaccgg tgaaggtaag acacttgttt ctacacttcc
    14521 tgcatacctt aatgcacttg agggcaaggg tgtgtgtatt gtcacagtca atgattacct
    14581 tgcaaagcgt gatgctgagt ggatgggaca ggttcatgag ttcttaggac ttaaggtcgg
    14641 cgtggtactt ggcggtatgg acaatgacga gcgcagggag gcttatggct gtgatattac
    14701 atatatcaca aacaatgagc ttggatttga ttacctgcgt gacaatatgg ttatctacaa
    14761 ggagcagctt gttcagagag gtctccacta tgctatcatc gatgaggtcg attcagtgct
    14821 tatcgatgag gcacgtacac ctcttatcat ttcaggacag agtggcaagt cgacaagact
    14881 ttacgaggca tgcgatatcc tggctacaca gatgaagcgt ggtgaggatg ttcctgagta
    14941 ttcaaagatg gatgccatca tgggcattgt ccaggatgag accggtgatt tcatcgtaaa
    15001 tgagaaggac aaggtcgtaa accttacaca ggatggtgtg aaaaaggtag aacagttctt
    15061 ccatatcgag aaccttgcag atcctgagaa tcttgaaatc cagcacaata tcatacttgc
    15121 gcttcgcgcg cacaacctca tgttcaaaga tcaggactat gtggtaaagg atgatgaggt
    15181 gcttatcgta gatgagttta ccggacgtat catgcctgga cgccgttatt cggacggact
    15241 gcatcaggct atcgaggcaa aggagcatgt aaaggttaag agagagagca agaaccttgc
    15301 cacaatcaca ttccagaact ttttcaacaa gtttgacaag aaggccggta tgacaggtac
    15361 agctctcaca gaggagaaag agttccgtga tatctatggc atggatgttg tcgagatacc
    15421 tacaaataag ccggtagcgc gtatcgattt gcaggatgct gtatacgcta ccaaaaagga
    15481 aaagttcaag gcagttgtgg atgcggttaa agaggcacat gaaaagggac agcctgttct
    15541 tgtaggtaca atcaccatcg agacatcaga gcttttgagc ggcatgctca agagagaggg
    15601 aatccctcac acagtattaa atgccaagtt ccatgagaaa gaggcagaga ttgttgcact
    15661 tgcaggacag catggtgcag ttacaatagc aaccaacatg gcaggccgtg gtaccgatat
    15721 taagcttgac gatgaggcaa aggctgccgg aggtcttaag attatcggta cagaacgcca
    15781 tgagtcaaga cgtatagaca atcagctccg tggacgtgca ggacgtcagg gagatccggg
    15841 tgagtcacag ttcttcatct cacttgagga cgacctcatg agacttttcg gttcagagcg
    15901 tctgatgggc gttttcaatg cgctcggagt accggagggc gagcagatcc agcataagat
    15961 gctcacatct gctatcgaga aggctcagga gaaaatcgag gccaacaact ttggtattcg
    16021 taaaaatctt ctcgagtatg accaggtgga caatgaccag agagagatta tctacagcga
    16081 gcgtatgaag gtgcttaacg gtgacaacat gcgtgatgct atccttaaga tggttcagga
    16141 acaggttgag aagtctgtcg acagatgtat ttccgaggaa attgacagag ctgactggga
    16201 tcttgtagag ctcaacgagc ttgtgcttcc ggtcattcct cttgatccga ttacaaaaga
    16261 ggatattgag tcaatcaaga atgcaaagca gcttaagcag tacttaaagg aaaaggctgt
    16321 catgctttat gagcagaagg agacagaatt ccctgagcct gagcagttca gagagattga
    16381 gcgtgtcgtg cttcttcgtg taatcgacag aaagtggatg gatcacatcg atgatatggc
    16441 acagcttcgt cagggaatcg g
//
LOCUS       DPUF01000207            2591 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6709, whole
            genome shotgun sequence.
ACCESSION   DPUF01000207 DPUF01000000
VERSION     DPUF01000207.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6709"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="DHV81_07400"
     CDS             <1..636
                     /locus_tag="DHV81_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94277.1"
                     /translation="EFGFLPLFKNEIDGFSLEERTVPEYWWSDNPEIDPWMWRAIIAR
                     RHDIVYGKFFDKKAGFISKKWLPVFANYRRDGYDFDALYDDGKAPNKHKKIMVNFMED
                     NADSEIYSNELKKQAGFGKNGEKGFDGAITNLMMQTYLCNCDFKKRVNKRGIEYGWDV
                     AVYSSIEHIYGYDYVTSCYKDNPQDSWKQIVDYMHEMYPEATDKQIRKLLK"
     gene            664..1728
                     /locus_tag="DHV81_07405"
     CDS             664..1728
                     /locus_tag="DHV81_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94278.1"
                     /translation="MPFKIVRNDITKVKADIIVNTANPNPICVSGTDLAIYEAAGKEK
                     LLAERANIGKIARGDIAVTGAYSLNAKYIIHTVGPVWTDGLHHEFEILEDCYRKSLQK
                     ALELKCDSIAFPLISTGVYGFPKDKALQIAVSVFSRFLTENEIEIILVVFDKRSFQLS
                     GQIVGDIDSYIDANYVKESHRKEYPVRSRSNARMRELPEEAFYEEMLQREAEDNYPLE
                     EDTGLAEPCMLSADISLEDQLANIGVSFHDKLFELIDESHIDNKDVWKRANLDRKHFS
                     KIQCDRNYHPKKKTVMALCIALQLDLKQSRDLLARADWAFSPSSKVDLIVQKAIIDKQ
                     YDIMQLNVTLFKYTNEILGV"
     gene            1709..2029
                     /locus_tag="DHV81_07410"
     CDS             1709..2029
                     /locus_tag="DHV81_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94279.1"
                     /translation="MKFLEYRSKIDMWDNIYKPNSIGSEGGTIIADEEYKESCRITLE
                     RCERYDAITCGVYGCMMHTAFCNKSHSQEVFDNMKKDLQEFIDKDTTADEEIIFYEEF
                     TSKY"
     gene            2188..>2591
                     /locus_tag="DHV81_07415"
     CDS             2188..>2591
                     /locus_tag="DHV81_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94280.1"
                     /translation="MYGAILGDIIGSPFEFDRGDKTKNFDLFSEGCGFTDDSVMTIAV
                     GEALLAVGPKAAVKEIEDAVASNMQDWGKRYPHAGYGGSFRHWLKENNPKPYGSYGNG
                     SAMRVSAAGWLYDSIERTREVARATANVTHNHP"
BASE COUNT          882 a          352 c          636 g          721 t
ORIGIN      
        1 gaatttggct ttttaccatt atttaagaat gaaatagatg ggttttcact tgaagaaaga
       61 actgttccag agtactggtg gagtgataat ccggagattg atccatggat gtggcgggct
      121 attatagcaa ggcggcatga tatcgtatat ggaaaattct ttgataagaa ggcaggattt
      181 atatcaaaaa agtggcttcc tgtatttgct aattatcgcc gggatggata tgattttgat
      241 gcattatatg atgatggaaa agcgcctaat aagcataaaa agataatggt caattttatg
      301 gaagataatg ctgattcaga gatttattca aatgaattga aaaaacaagc tggatttggt
      361 aagaatggag aaaagggatt tgatggtgct ataacgaatc ttatgatgca gacctatctg
      421 tgcaactgcg attttaagaa gagagtgaat aaaagaggta ttgaatatgg atgggatgtg
      481 gcagtgtatt catctattga gcatatctat ggatacgatt atgtgacttc ctgctacaag
      541 gataatccac aggattcatg gaagcagatt gtagattata tgcatgagat gtatccggag
      601 gcaacggata agcagataag gaagctgtta aagtaataca ggcatggata aggaggtgcc
      661 acaatgccat ttaagattgt tcgcaacgat ataacaaagg taaaagcaga tataatcgta
      721 aatactgcta atccaaatcc gatatgtgta agtggcacgg atttagcaat atatgaggca
      781 gcaggaaaag aaaagcttct cgcagaaaga gcgaatatcg gcaaaattgc aagaggtgat
      841 attgctgtca ctggtgctta tagcctaaat gcaaaatata ttattcatac ggtaggtccg
      901 gtatggacgg atggattgca ccatgaattt gaaattttgg aggactgcta tcgtaaatca
      961 ttgcaaaaag cattggaact taagtgcgat agcattgcat ttccattaat ttcgacagga
     1021 gtatatggat ttccaaagga taaggcattg cagattgctg tgtcagtatt cagccggttt
     1081 cttaccgaaa atgaaattga aattatattg gtcgtgtttg ataaaaggtc ttttcagcta
     1141 tccggtcaga ttgtaggtga tattgattcc tacatagatg ctaattatgt caaggaaagt
     1201 catagaaaag aatatccggt gcgaagcaga agcaacgctc gcatgagaga attgccagag
     1261 gaagcgttct atgaggaaat gcttcaaaga gaagcagaag ataactatcc acttgaggaa
     1321 gatacagggt tagcagagcc ttgtatgctg tcagcagata tttctttgga agaccagctt
     1381 gcaaatattg gtgtttcatt tcatgataaa ttatttgagc tgatagacga atcgcatatt
     1441 gataataagg atgtgtggaa acgggcaaac cttgatagaa aacatttttc aaagattcaa
     1501 tgtgaccgga attatcatcc aaagaagaag actgtgatgg cactctgcat tgcactgcag
     1561 cttgatctga aacagtcaag agatttactt gcgagagcag actgggcatt tagtccaagc
     1621 agtaaggtgg atctgatcgt acagaaggct ataatagata agcagtatga tattatgcag
     1681 ctgaatgtga cattatttaa gtatacgaat gaaattcttg gagtatagga gcaagattga
     1741 tatgtgggat aacatatata aaccgaattc tattggcagt gagggtggca cgattattgc
     1801 agatgaggaa tataaagaat cgtgtaggat aactttggaa agatgtgaaa gatatgatgc
     1861 aattacatgt ggagtatatg gatgtatgat gcatacagct ttttgtaata aaagtcatag
     1921 tcaggaagta tttgataata tgaaaaaaga cttgcaggaa ttcatagata aggatacaac
     1981 agcggatgaa gaaattattt tctatgaaga gtttacaagt aaatattgat tagtaaatgt
     2041 tacgtcggta ctcacatagt atcggcgttt gttttttatc ttaaaagtgt cgcccgaata
     2101 gcgaccatat aaagatgtca ttcaattata ctcatattgt aacagagaaa gagaggtgcc
     2161 ggagtaatcc ggggattgga ggcatatatg tacggagcga ttttaggaga tattattgga
     2221 agcccgtttg agtttgacag aggtgataaa acaaagaact ttgatttgtt tagtgaaggc
     2281 tgtggcttta cagatgattc tgttatgaca atagcagttg gagaagctct cctggcagtc
     2341 ggaccaaaag ctgcagtaaa ggaaattgaa gatgcggtag catccaatat gcaggattgg
     2401 ggaaaaagat atccacatgc aggttatggt ggaagcttca gacattggct taaagaaaat
     2461 aatccgaagc catacggaag ctatggaaat ggttcagcca tgagagtgtc agcggcaggc
     2521 tggctttatg attcaattga aagaacaaga gaggtagcaa gagcgacagc aaatgtgaca
     2581 cataatcatc c
//
LOCUS       DPUF01000208            2705 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_17736, whole
            genome shotgun sequence.
ACCESSION   DPUF01000208 DPUF01000000
VERSION     DPUF01000208.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_17736"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="DHV81_07420"
     CDS             complement(<1..1052)
                     /locus_tag="DHV81_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HCI94281.1"
                     /translation="MSKKIIVVGGGAAGMLAAYFAAAAGNKVTLLEKNEKLGKKIYIT
                     GKGRCNLTNACDVEELFLNVKSNSKFLYSAFYGFDNSRVIDFFESHGMPVKVERGNRV
                     FPVSDKSSDVIFALQKALKEKNVEVLLHTEVSKLCYEKITDTKADEEASDKKPSDKKP
                     SDKKPELKITGVILKDGTKMDADAVIVATGGLSYPSTGSTGDGYKMAESAGHTVTECT
                     PSLVPFNVKEEWVKSLQGLSLKNTAISIYSGKKKLYEDFGEMLFTHFGVSGPMILSAS
                     ASIKQSLIKQPLDMYIDLKPALTQEALDKRILREFEEAKNKQFKNSINKLLPAKMIPV
                     IIELSGIDPDKKVNEIS"
     gene            complement(1045..1974)
                     /locus_tag="DHV81_07425"
     CDS             complement(1045..1974)
                     /locus_tag="DHV81_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCI94282.1"
                     /translation="MSNTNDLLNRINAKYSTMSKGHKLLATYITDNYDKAVFLTAAKM
                     GETVGISESTVVRFATSLGYKGYPDFQKALEELVRNKLNSVQRMEVTYGRISQSQILE
                     TVLQSDRDKIKTTLEKIDADAFDLAVDTILQSRKIYIVGIRSCAPLASFLAFYMNLMF
                     EDVCLLTTNSSSELFEQMVRIGNEDVIIGISFPRYSMRTLKALEFANNRSAKVITITD
                     SIHSPMNLYSSCNLIADSDMASIVDSLVAPLSVINALIVALCMKKQSIVAKTLTELED
                     IWNEYQVYENDEIDYIDDSIKVHYAKLGDSQDE"
     gene            complement(2475..>2705)
                     /locus_tag="DHV81_07430"
     CDS             complement(2475..>2705)
                     /locus_tag="DHV81_07430"
                     /EC_number="4.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882851.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HCI94283.1"
                     /translation="LGDDKKNAITCRPADLLEPELDKIEAEMKQWKQQDEDVLSYALF
                     PQVATDFFKYRQAQQTGVDAKAADTENKAYPV"
BASE COUNT          730 a          677 c          484 g          814 t
ORIGIN      
        1 gatatctcat tgaccttctt atccgggtca atgcctgaaa gctcaattat caccggaatc
       61 atttttgcag gcaaaagctt gtttatcgaa ttcttaaact gcttgttttt tgcctcctca
      121 aactccctga gaatcctctt gtccaaggcc tcctgtgtga gagccggctt caaatcaata
      181 tacatgtcaa gaggctgctt aataaggctt tgcttaattg atgcactcgc agacaaaatc
      241 atagggccgc tgaccccaaa atgcgtaaag agcatctctc caaaatcctc ataaagcttc
      301 tttttgccgc tatatatgga aatagccgta tttttaaggg aaagtccctg aagactcttt
      361 acccactctt cctttacatt aaatggcaca agcgacggtg tgcactctgt cacagtatga
      421 cctgcgcttt ctgccatttt gtagccgtca cctgtagagc ctgtagaagg atatgaaagt
      481 cctcctgtgg ccacaataac tgcatcagca tccattttgg tgccatcctt taatatgaca
      541 cctgttattt ttaattcagg ctttttatca ctaggctttt tatcactagg ctttttatca
      601 ctagcttctt catctgcctt tgtatcagtg attttctcat agcacagctt tgatacctcc
      661 gtgtgaagta atacctccac attcttttcc ttcaaagctt tctgtaaagc aaaaatcaca
      721 tccgacgact tatcagatac cggaaataca cgattgccac gttccacctt tacaggcatg
      781 ccatgactct caaaaaaatc aatcaccctg ctgttgtcaa agccataaaa agcgctgtac
      841 aaaaacttac tgttgctctt tacatttaaa aaaagctcct cgacatcgca cgcattggtc
      901 aggttgcatc tgcccttgcc ggttatatat attttcttcc caagtttttc atttttttca
      961 agaagcgtaa ccttatttcc tgcggctgca gcaaaatacg ccgcaagcat tccggcagct
     1021 ccgccaccta ccacaataat tttcttactc atcctgcgaa tcccccagct tagcgtaatg
     1081 tacctttata gagtcatcaa tgtaatcaat ctcatcattc tcgtacacct gatactcatt
     1141 ccatatatcc tcaagctctg tcagtgtctt tgcgacaata ctctgctttt tcatgcaaag
     1201 agccacaatc aaggcattga tgacagaaag cggagcaaca agtgaatcaa ctatagaggc
     1261 catatcactg tcggcaatca gattgcatga agaatacaga ttcatcggtg aatgaatgct
     1321 gtctgtgata gtgataacct ttgcactcct gttgttggca aattccagtg ccttaagagt
     1381 acgcatagaa taacgcggaa agctgatgcc gataatcaca tcctcgttgc caatacgcac
     1441 catctgctca aaaagctcgc tcgagctgtt tgtcgtaagc agacacacat cctcaaacat
     1501 cagattcata taaaatgcaa gaaagctcgc aagaggtgca cagcttctga ttccgacaat
     1561 ataaattttc cttgactgta aaatagtatc taccgccagg tcaaatgcat ccgcatcaat
     1621 tttttccaga gtggtcttaa tcttgtcacg atcagactgc agtacagtct caagaatctg
     1681 tgactggctg atacgcccat atgtaacctc cattcgctga accgaattga gcttgttcct
     1741 tacaagttcc tcaagtgcct tctgaaaatc gggatatccc ttatatccga gcgatgtagc
     1801 gaacctgaca accgtagact cagaaattcc taccgtctcg cccatcttag ccgctgtcag
     1861 aaagacagcc ttatcataat tatcagttat gtatgtagca agcagcttgt gccccttgct
     1921 catcgtactg tatttagcat ttatcctgtt aagtaagtcg ttagtatttg acatcttata
     1981 ctcctgagct ttcccgttta tggctgtgct tcacacattt gtgtgctttg cagcagtttt
     2041 tccatacctt aaaatattat cacttttttc ctctatatga aagagcgaat ttatattttt
     2101 tatttttgcc atagaataga ctcgtaagtc ccgggcaccg ggcaggcacg cacactttcc
     2161 cgtgcgctac gctacgtctc taagatactg tacgaaatta tgtttgtagt tgttctatgc
     2221 ttgacgaaac cggcttttat gcgccatcca tggcgcataa caccttgtcc tgccgtccat
     2281 ggcaggacat ttctgtgttt catggacata atttctacag tatctaagag gctgcgctat
     2341 tcgcgcacat gaaagtgtgc atgcctttcc ggcgccctgg acttacgagt caccctatcc
     2401 acataatata aaaaacaggc ccggcaaagc cggacctgct tgaaattcaa aagcatgaat
     2461 tatattttta aatactatac aggataagcc ttattctcag tatcagcggc ctttgcatca
     2521 acacctgtct gctgtgcctg tctgtacttg aagaaatcag tagcgacctg tgggaaaaga
     2581 gcataagaaa gaacatcctc atcctgctgt ttccactgct tcatctcagc ctcaatcttg
     2641 tcaagctctg gctcaagaag atctgccgga cgacatgtga tggcattctt cttatcatca
     2701 ccaag
//
LOCUS       DPUF01000209           17367 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3991, whole
            genome shotgun sequence.
ACCESSION   DPUF01000209 DPUF01000000
VERSION     DPUF01000209.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 17367)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17367)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17367
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3991"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="DHV81_07435"
     CDS             <1..981
                     /locus_tag="DHV81_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCI94284.1"
                     /translation="ETVHAHLPALHRYMALRKKLLGVEHLHMYDLFAPIVDNVQTTYT
                     YDEAKKLVAKALEPMGDEYVSTLKAGMESGWIDVYENENKRSGAYSWGAYGTHPYVLL
                     NYADNLDSVFTLAHEMGHAMHTYYSNEHQSITYAGYLIFVAEVASTCNESLLMHYMLE
                     HCEDENERKYLMTHFLDGFRTTLFRQAQFAEFEHIAHRKMQKGEPVTKDVLNELWHEL
                     NVQYYGPDMRVDDEISYEWMRIPHFYTPYYVYQYSTGYSAAVAFSKKILEEGKPAVDK
                     YIGNFLCGGCSKNPIELLKSAGVDMSTPKPVDDALNVFEDYLKQFETATK"
     gene            996..1454
                     /locus_tag="DHV81_07440"
     CDS             996..1454
                     /locus_tag="DHV81_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCI94285.1"
                     /translation="MERIKSFTINHNILKPGFYISRKDGEIVTYDLRTRKPNAGDYMS
                     NSAMHSVEHMFATCVRNSEIADDVVYFGPMGCQTGFYLVVRDVEPGKVFEITKEVLKQ
                     ILEYDGPVFGASAIECGHYENLSLDEAKKECRAYLNVLESQTNMEFTYPQ"
     gene            1503..2198
                     /locus_tag="DHV81_07445"
     CDS             1503..2198
                     /locus_tag="DHV81_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCI94286.1"
                     /translation="MTIGVIGAMQMEVDDLKAQMTDTQTEVYSGVEFVEGKIGDQYVV
                     AAKCGIGKVFAAICAEAMILKYDVDMIVNIGVAGTLTKDLNVLDVAVATNVLQHDMDT
                     SPIGDPKGLLSGINMILLPADTKMVELMCSCLADRNIHYKKGNIATGDQFIATKEQKD
                     YISNTFDAIAAEMEGGSIGHVCYVNKVPFAILRSISDGDGGETDYQTFAEKAAHQSIE
                     VVLEFIGRAEELK"
     gene            2247..3098
                     /locus_tag="DHV81_07450"
     CDS             2247..3098
                     /locus_tag="DHV81_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitooligosaccharide deacetylase"
                     /protein_id="HCI94287.1"
                     /translation="MSEYRAYMQNTKLHNGTKRKKRRHRFLFGVLLSALLITGALYGR
                     EYDISQLKNIEVMETLSSAVSQFGDRKLPVYSVQTTEKKIALSFDCAWGAEDFDSMME
                     TLDKHNVKATFFMTGGFVSDNPECVKTLVEKGHEPGNHSEHHYDMATITAGEMKTEIM
                     DVHKKVKELTGKDMKVFRPPYGSYNNELIDTVYGCDYYPIQWDVDSLDWKNYGVQNII
                     DTVCNHKSLDCGSIILCHLGAKYTSQALDEMLTKLQDMGYEIVPVSKLIMTEGYHMDA
                     NGMQIKD"
     gene            3643..5655
                     /locus_tag="DHV81_07455"
                     /pseudo
     CDS             3643..5655
                     /locus_tag="DHV81_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568254.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acriflavin resistance protein"
     assembly_gap    4122..4205
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4396..4469
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5656..5844
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5845..7449
                     /locus_tag="DHV81_07460"
     CDS             <5845..7449
                     /locus_tag="DHV81_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acriflavin resistance protein"
                     /protein_id="HCI94288.1"
                     /translation="MLKKTKDIKHPWFDRFKDWYGNVLGTCLRFKPVVFIAAVVLLVG
                     SGALCMSRGMTFMDMDMESNQISLTIEAKDDEKLDFKQLTELSDKVMDKISDIKGIDT
                     IGATAGGSSTMNLMSSGSDSVSMYILLDEKSDVSSDKVIDEIEKRTKNLDCKVTASSQ
                     AMDSSEFFGSGLSVRIKGNDIDKLQKLAGDVADILKDTNGVTDIDDGLGDTINELKIT
                     VDKEKAAKYGYTVAQVYQLVSGEISSQKSATTITTDIKDYEVYLQSAEQSDVTVDDIK
                     NLTFTYKDNDGSEKEIPLSDICTISQEQTLSTIHRDAQTRYITVSGAVDENHNVTLLS
                     NKIKSKIDKLSIPDGYSIDMEGEDETINESMSQLGLMLVLAVIFIYLIMVAQFQSLLS
                     PFIIMFCIPLAFTGGFIALFITGNELGIISMLGFIMLAGLIVNNGIVLIDYINQARRE
                     GMSKHEAIIDAGRTRLRPILMTAMTTILAMFTSAIGIGDGSDMIKPMAIAIIGGLVYG
                     TILTLIVIPCIYDAFNREKSMVEEEL"
     gene            7784..8470
                     /locus_tag="DHV81_07465"
     CDS             7784..8470
                     /locus_tag="DHV81_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCI94289.1"
                     /translation="MKYFLDSAKIEEIKEAYHTFGIDGVTTNPRHIMVSGKPFLTVIS
                     EMADWVKAEGIEGYEKFPISVEINPHLDDADEMVAEARKISAICSNFVIKIPCTEAGI
                     QAARRLEAEGIRTNVTLVFSPAQAILPAKNNSLFVSPFIGWKEANGEDCKAYIQAISD
                     IYDNYGYKGKTQIICAAIRTPKQIVDCAVAGADIVTTGLAVYKDSIKHAYTRQGLETF
                     QNAWDNTVTE"
     gene            8497..9360
                     /locus_tag="DHV81_07470"
     CDS             8497..9360
                     /locus_tag="DHV81_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCI94290.1"
                     /translation="MKIGFVGLGIMGESMSENIVKKHDDEVYVFDFVKEKVELLESKG
                     AIGCSSSLKLAEKSDVIISMVPKSEHSMAVYKEVLPALDDTKLCIDMSTIDPSVSVEI
                     SEMVKKTGAEFVDAPVVKSKPAAIAGKLGIYVGGDEKACEKAMPILSYMGENIIRMGG
                     NGKGLVMKICHNALVSQIQNGVNETLTLARAYGIDIESYTTAISYGGGQNFYLDSKNV
                     PIKNEDYTTAFSIENMYKDVNICMNMAKESGLKLQGENAAKQVYDKAMELGFGKEDFC
                     ATIKAVKAMQR"
     gene            9372..10043
                     /locus_tag="DHV81_07475"
     CDS             9372..10043
                     /locus_tag="DHV81_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 domain-containing protein"
                     /protein_id="HCI94291.1"
                     /translation="MLKHLNEKNSVTIYSVTDPCFETYGRVISNINCNELISFMEQNT
                     VIPEKGNIYVASVEELERTHIAEFFKNRIYGEMPIQIGYCNGRNTTYNGFEYHKGSEI
                     NIAVTDFMLVLGHTWEIKDNTYRIEDAKVFFVEKGTAIEMYQTTLHLSPCRVSDIGFK
                     DVVILPRGTNTPLNNGGTDEAAIGEERLLLQRNKWVIAHPDREPLIKQGAYPGLLGEN
                     KELYY"
     gene            10204..11949
                     /locus_tag="DHV81_07480"
     CDS             10204..11949
                     /locus_tag="DHV81_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCI94292.1"
                     /translation="MEHLVFTLVTFVLFTALVGIISGKIVKNSDDQKTAKGYFLAGNG
                     LGGFFIAGSMLLTNLSAENLVGLSGQSYAANMSGMAWEATAVIATIIMAFVFLPMYLR
                     KGYTTLPEFMEERYGVGVRRMVSILFLIGYLVIGIPVCLYAGAIAFEQIFDFATVFGI
                     SEGMALTILIVLVGIIGALYAVLGGMRAVAVSDTINGILLVLGSVLVVVFGFIAVGKL
                     SGGGFIVGVKDVITYEPAKLNAIGGKNDPVPFACIFTGMLLANLFYWGTNQVLIQRAL
                     GAKNLKEGQKGVLLSGFLKMMVPLLLVIPGVIAARLVPGLTSKDFAYPSLVARSLPWP
                     VVGLFCAALFGSIISTYNSFLNAASTVFMIDIYKPIFNPNLSEEATVKLAKKIGWGFA
                     AFAIIFTPFLDVLSTGLYDFGRSFTGFYNIPIITMVLVGMFSKKGSKLGAICATIFHI
                     AFYACYKFIFPHIDTPFTNAVSGINYMYIYAISFVIMLVIIFVCSKIAPNTKVFDKSA
                     SRNDGYDMTAWKHKNAFAIWLVSFLVYEYFIFSPAGIATENKNVVRIAIVTAIFVIET
                     IVLIVKEMNKRKKTA"
     gene            11962..13968
                     /locus_tag="DHV81_07485"
     CDS             11962..13968
                     /locus_tag="DHV81_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCI94293.1"
                     /translation="MQIKVNDNEFQLFVGEKRILEHSKERPMIYVGVGQEDVDMYRGN
                     FKITDYVTERFPLKLTDVIQTADTVRLCFESYIIAKIKCDENLCTIDFEQKDDRINRF
                     WFRVAADKEEKCYGCGEQMSYFNLRGRNFPIWTSEPGVGRDKTTYVTWRSDVENKAGG
                     DYYNTNYPQPTFVSTNKYYLHVDSTAYADFDFRNDSFHELQIWEVPKQIRIECADTYL
                     KLLERITTYFGRQPKLPDWVYNGLIIGVQGGNERSFGLLDKTLDRNIKVAGIWCQDWC
                     GKRVTSFGKRLQWDWKYHKEMYPDLPKKIKEINAKGIKFLGYVNPYLVNDGELYKEGK
                     EKGYFATKADGSDYLVDFGEFYCGVVDLTNPEAFEWFKDIIKEYTLGIGIDGWMADFG
                     EYLPTDDICLYSGKSPMIEHNHWPVLWAKCNYEAVKESGKLGDVVYFMRAGGAGSQKY
                     CTLLWAGDQSVDFTIHDGLASVICGALSAGMMGCGLTHSDIGGYTSLFDNTRTKELFL
                     RWAEMAMFTPFMRTHEGNRPDTNFQYYDDEDTMERLARLVDVYTMLAPYTKTLVEENA
                     DSGHPVQRPLFMHYESDAKAYDIQYEYLFGRDMLIAPVYEQDKHEWDVYLPQDEWVHL
                     WTGEEYHGGEITVSAELGYTPAFYRKNSEFADIFEEIREKYGVK"
     gene            13969..14703
                     /locus_tag="DHV81_07490"
     CDS             13969..14703
                     /locus_tag="DHV81_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCI94294.1"
                     /translation="MNTVLFYIIIFLTNVIQGITGFAGTILAMPPSLILVGYDVAKPI
                     LNVLGILAGVYVFVTQWKHVDWKQLKKVVIVMAIGIFGGFFLKNALAGYDAVLYKFLG
                     IFVIFLAIHGFYSIYGQRQRDTKEKSSWKSMLLLIGAGIVHGMFVSGGPLLIGYLSNE
                     IKEKTSFRATISTVWIILNSMILVQDICAGMWNAGLVMTQCISIPFLIAGMAAGSVLV
                     KRMSQEIFMKLTYILLFISGILLMVK"
     gene            14734..16074
                     /locus_tag="DHV81_07495"
     CDS             14734..16074
                     /locus_tag="DHV81_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HCI94295.1"
                     /translation="MTKFQVAYVPVGVPTFHLESAQKEFEKSIDLLNTITDAAIVPDK
                     MLLSIQDLTNFLDTIEPSLIILQNITFANAAYASEVLKRFSCPVLLWTLREPVIDGGR
                     LRLNSLTGAYSAGNALAAFRDGNFEYIFGAPQEERTKKTISACIRAAWLKNELKNLRI
                     ASVGHTPQGFGFGRAMDADMQKVFGATLESIEVRELIDMAKGYTDDECSEYLDKMTKC
                     TVGLDKTPDNNRKDFARLCKAYKEYVDENNIGALSSRCWPDFFTAFGTPVCGVLAMLN
                     DMGVAASCEADTYGALSMYMGMKLSEQAVFFGDPVSLDESENTITFWHCGTAACSIAR
                     EDTGATIGVHPNRKIGPVMDFGCKAADKVTVFRVGQKADGSFRFFVTTGSALDKPKQF
                     CGTSIVVRTDKNSYDIVNQSVKDGWEPHFVVIYGDCSDELKVLGHMLEIEVCEY"
     gene            16154..>17367
                     /gene="glpK"
                     /locus_tag="DHV81_07500"
     CDS             16154..>17367
                     /gene="glpK"
                     /locus_tag="DHV81_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HCI94296.1"
                     /translation="MAKYIMALDAGTTSNRCILFNEKGEMCSVAQKEFTQFFPKPGWV
                     EHDAEEIWATQLEVAKEAMANIQATAADICAIGITNQRETTIVWDKNTGEPVYHAIVW
                     QCRRTAEYADSLKEKGLTETFRKKTGLVIDAYFSATKLKWLLDNVPGARERAERGELL
                     FGTVETWIIWKLTQGQVHVTDYSNASRTMMFNINTLKWDDEILKELDIPKSMLPKPMP
                     SSCVYGEVNPVYFGGPIPIAGAAGDQQAALFGQTCFRAGEAKNTYGTGCFLLMNTGEM
                     PVSSKNGLVTTIAWGIDGKVVYALEGSIFVAGASIQWLRDEMKFIDSSTDSEYMARKV
                     KDTNGCYVVPAFTGLGAPYWDQYARGTIVGLTRGVNKYHVIRATLESMAFQVNDVLEA
                     MKADSGINLTSL"
BASE COUNT         5358 a         2831 c         4243 g         4588 t
ORIGIN      
        1 gagacggttc acgcacatct gccggctctg cacaggtaca tggcgcttag gaagaagctt
       61 ttaggtgtgg agcatctgca tatgtatgat ttgtttgcac cgattgtgga caatgtacag
      121 accacctaca cctatgatga ggcgaaaaag cttgtggcaa aggcgcttga gcctatgggc
      181 gatgagtatg tcagcacact taaggctggc atggagagtg gctggataga tgtatatgag
      241 aatgagaaca agagaagcgg tgcgtattca tggggcgctt acggcactca tccgtatgtg
      301 cttttgaact atgccgacaa tctggacagt gtgtttacgc tggctcatga gatgggacat
      361 gcgatgcata catactattc aaatgagcat cagagcatca cctacgcagg atacctgatt
      421 tttgtggcag aggttgcatc cacctgcaat gagtcgcttc taatgcacta tatgcttgag
      481 cattgtgagg atgagaatga gagaaaatat ctcatgacac attttctgga cggcttcagg
      541 acgacacttt tcaggcaggc gcagtttgct gagtttgagc atatagctca ccggaaaatg
      601 caaaagggcg agccggttac caaggatgtg ctaaatgagc tatggcatga gctgaatgtg
      661 cagtactacg gaccggatat gcgcgtggat gatgagatat catatgagtg gatgcgcata
      721 ccgcattttt acacaccgta ctatgtatat cagtactcga caggctattc ggcagcagtt
      781 gctttttcaa aaaagatact tgaggaggga aagcctgctg tggataagta tataggaaat
      841 ttcctgtgcg gtggatgctc gaagaatcca atcgagctgc taaagagtgc cggtgttgat
      901 atgagcacac caaagcctgt ggatgatgca ctcaatgtat ttgaggatta tctgaagcag
      961 tttgagacag caactaagta aggagaaaca tttttatgga aagaattaaa agctttacaa
     1021 ttaatcataa tattcttaaa cctggatttt atatttcaag aaaagacgga gaaatcgtta
     1081 catatgacct tcgtacaaga aaacctaatg caggagatta tatgtccaat tcagctatgc
     1141 actcagtgga gcatatgttt gcaacatgtg tgagaaactc tgagattgca gatgatgtgg
     1201 tatattttgg accgatgggc tgtcagacag gcttctatct cgttgtgagg gatgtcgagc
     1261 cggggaaggt ttttgaaatc accaaagagg tcttaaaaca gatacttgag tatgatggac
     1321 cggtatttgg tgcatcagcc atagagtgcg gacattacga gaatctgtca cttgacgagg
     1381 caaagaagga gtgccgtgca tatcttaatg tgcttgagag ccagacaaat atggagttta
     1441 cttatccaca gtaaacgagg actaaaaata taatagcaag atagcaggaa tggagaaata
     1501 aaatgacaat aggtgtaatt ggtgcaatgc agatggaggt cgatgactta aaggctcaga
     1561 tgacagacac acagacagag gtttacagcg gagtggagtt tgttgagggt aaaatcggtg
     1621 atcagtatgt agttgcggca aaatgcggaa taggaaaggt atttgcggca atatgtgcag
     1681 aggccatgat tttgaaatat gatgtagata tgattgtcaa tatcggagtg gcaggaactt
     1741 tgacaaagga cttaaatgtg cttgacgtgg cagtagccac aaacgtgctg cagcacgata
     1801 tggatacatc gccaattgga gatccgaagg gacttctctc aggcatcaat atgattctgc
     1861 ttccggcaga tacaaaaatg gtggagctta tgtgctcatg cttagctgac aggaatatcc
     1921 actacaaaaa gggcaatatc gcaacaggag atcagtttat tgccacaaag gagcagaagg
     1981 attatatttc gaataccttt gatgcaatcg cagcagagat ggagggaggc agcatcggtc
     2041 atgtgtgcta tgtgaacaag gtgccatttg ccattcttcg ctcaatatca gacggcgatg
     2101 gcggcgagac agactaccag acctttgcgg agaaggctgc gcatcagtcg attgaggtag
     2161 tgcttgagtt tatcggaagg gcagaagaac tgaaatagag ttatttcatt attcataatt
     2221 tgtgataata aagaatagta tatagcatga gtgaatacag agcatacatg caaaatacaa
     2281 aactacataa tgggacaaaa cgaaagaaac gccgacacag attccttttt ggagtgctgc
     2341 tgtcggcgct tcttattaca ggcgctttat atggcagaga gtatgacata tcacagctga
     2401 aaaatataga ggtgatggag acacttagca gtgctgtgag tcagttcgga gacagaaagc
     2461 tgcctgtata ttcggttcag acaacggaaa aaaagatagc cctttcattt gactgtgcct
     2521 ggggagcaga ggattttgac agtatgatgg agacacttga caagcacaac gtaaaggcta
     2581 cattttttat gacaggaggg tttgtgagtg ataatcctga atgtgtaaag acgctggttg
     2641 aaaaagggca tgagcccgga aatcacagtg agcaccacta tgatatggca acaatcacgg
     2701 caggcgagat gaagacggag ataatggatg tgcacaaaaa ggtcaaggag ctgaccggaa
     2761 aggatatgaa ggtattcaga ccgccatacg gctcgtacaa caatgaactg attgacacag
     2821 tttatggctg tgactattat ccaatccagt gggatgtgga ttcgctcgac tggaaaaact
     2881 atggtgtgca aaacatcata gatacagtgt gcaatcacaa aagcctggat tgtggctcga
     2941 taatactctg ccatctaggc gcgaaataca cctcgcaggc actcgatgag atgctcacga
     3001 agcttcagga catgggctat gagatagttc cggtatcaaa gctgatcatg actgaaggct
     3061 atcatatgga tgcaaacggt atgcagatca aggactgagc aatttcataa atcaatattt
     3121 tagtcaatat ttagtcaata atttagccct ttacgatatt gttatttaca gacaagtgtg
     3181 ttaaactaaa gctatatttt aaagaagctg tgagacagct atgggggtaa attatatgac
     3241 agatacaggt tttttagtag cattctttat agttattttt ttagttatca tctttgcgct
     3301 tgtcgctgta atcggggccg ttacaagtgc tgttgcaggt atcaatgatg atgaggatga
     3361 gattgaggaa gagggcatct gaagctaaaa taaataaaag atattaaaag atattaaaag
     3421 atatttaaag acatttaagg acatcgaatt atattaatcg tataattggg tgtcttttta
     3481 ttgtcaaaat ctcttaaaaa agtataaacc aattgagttg caatccgaca cattgtataa
     3541 taaaagtata acagcgaatt aaatatattg tgaaaatcgc agtaatcatt gtctattaaa
     3601 ctaatgcagg acattatcaa gaaaagaaaa aggaggggtt agatgatatc cagatatagt
     3661 gtaaaaaagc cttttactgt acttgtagga gtattgctgg taatagtact tggtgttgta
     3721 tcgcttggca ggatgacgac agatctgctg ccggatatga gctttcagta tgcacttatc
     3781 gtgacaacag atatgggagc aagtccacag gaggtggaga gcgatgtcac cgcgccgctt
     3841 gaagcagcta tggcaactac gtcaaatatc aaaaacgtaa gctcaatgtc atataacagc
     3901 tattcgctgg tgacatgcga gtatgagcag aatgccaaca tggattctgt agtgatagag
     3961 atgcagcaga aaatagatca ggtcaaggct ggctggaagg atggagtggg tactccgacc
     4021 attatgaaga taaatccgga catgcttcca atcatgatga ctgctgtgga tatggatggg
     4081 aaaagcaagt cggaaatcac agactttgtg gaaaacacac tnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnngagca gatacaggtc acgatgaatc aggccaagat agatgcgctc aatgagaaaa
     4261 taaataatgc cgtttcaaag aaatttgacg atgcaaaaag tcagatggat gatgcaagcg
     4321 caaagataga gagcggacaa aacgcaatca catcggcatc ggatcagatg agcgcgggag
     4381 tggatacgct aaaggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aagcttaaat gagcagaaga gccagcttga
     4501 aacaattcag tcagctcttt cagacttcat gaatagtgat acatatacaa agacaattcc
     4561 gtcactcaaa gagggcgcaa atgcaccggg tgaagcgggc acggcactta aagcacagct
     4621 tgagcaggtt aataagcaga ttgcaacaca gttttcaggt ctctcggcgc ttggaatcaa
     4681 ggtaaacaca gcagatgatc tgcctgcggc agcgtcagcg atagcacaga ctctcattca
     4741 ggtgaataca ggtatagagc agtgcaggag tgggcttgac cagatagcac aaggagagac
     4801 agcactttta gatgcctatg ataatctaaa cagtcaggca gctataagct cgataagcat
     4861 aggacagcag tcagcacagc ttgcaacagc agcggcatct cttgacagtt caaagaagga
     4921 gcttgaaaag agtaaggatg atgcacttga caagtcaaac ttaaatgcag ttttaacaat
     4981 tgactcgctc tcgcagttgc tcgtcgctca gaattttgat atgcctgcag gctatgtaaa
     5041 tgattcaaat ggcacgcagt acatagtaca ggtgggagat gagataaaat caattgatga
     5101 tttaacaaat ctggtactta tggatatgaa gcttgatgga atagctccta ttcatgtcgg
     5161 tgatgtggca gatgttgaga tgactgacaa ctcggatgag acatatgcga tagtcaacgg
     5221 taatcccgga ataatgctct ccatggagaa gcagaccggc tactctacag gagacgtcac
     5281 agaccgtctt ttagacaagc ttgatttgct ggaaaaggaa aatgacggat tacacaccac
     5341 agtgcttatg aatcagggcg tatacatcga catggtagta aagagtgtta tggaaaatat
     5401 gatagtggga gcaatacttg ccattttcgt attgcttttg ttcttaaagg atataaagcc
     5461 aacacttgtt atcgcagcat ctataccact atcggttatt gtggcagtgg tgcttatgta
     5521 ttttacggat atcaccttaa atatcatttc aatgtcggga cttgtgcttg gaataggaat
     5581 gctggtggat aattcaatag tagtaatcga gaatatctac agattaaggg gagagggata
     5641 ttcggtaaag aaggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnatgctc aaaaagacga aggatataaa gcatccatgg
     5881 tttgaccgtt ttaaggactg gtatggaaat gtgctcggca catgtcttag atttaagccg
     5941 gtggtattta ttgcggcagt agtgctttta gtcggcagtg gcgcactttg catgtcaagg
     6001 ggaatgacat ttatggatat ggacatggag tcaaatcaga tttcacttac catagaggca
     6061 aaggatgacg aaaagcttga cttcaagcag cttacagagc tgtctgataa ggtcatggac
     6121 aaaatatctg atataaaggg catagacaca ataggagcta cagcaggcgg cagctcgacc
     6181 atgaacctga tgagctcagg aagcgacagc gtgagcatgt atatattgct tgatgagaag
     6241 tcggatgttt catcggataa ggtaatagat gaaattgaaa aacgcacaaa gaatctggac
     6301 tgcaaggtaa cagcatcttc gcaggctatg gactcatcag agtttttcgg aagtggtctc
     6361 tctgtcagga ttaagggtaa tgatatagac aagctgcaga agcttgcagg tgatgtggca
     6421 gatatcctta aggacacaaa tggagtgaca gatatagatg acggtcttgg agatacaatc
     6481 aatgagctta agataactgt ggataaggaa aaagcagcaa agtatggcta tacggtggct
     6541 caggtatacc agctcgtatc cggtgagata tcatctcaaa agagcgcaac aaccattacg
     6601 accgacataa aggactatga ggtatactta caatccgcag agcagtctga tgtgactgtg
     6661 gacgacatca aaaaccttac atttacctat aaggataatg atggcagtga aaaggagatt
     6721 ccgctcagtg atatctgcac tatttcacag gaacagaccc tgagtaccat tcatcgagat
     6781 gctcagacca gatatattac agtatcaggt gcggttgatg aaaaccataa tgtaacatta
     6841 ctaagcaata aaattaaatc gaaaattgat aaacttagca ttcctgatgg ctattcaata
     6901 gacatggagg gcgaggatga gacaataaat gagtcaatga gtcagcttgg acttatgctt
     6961 gtgcttgcag tgatatttat ctatcttatc atggtggcgc agttccagtc attgctttca
     7021 ccgtttatta tcatgttctg tatacctctt gcgtttacag gaggctttat agctcttttc
     7081 atcacgggaa atgagctcgg aatcatatca atgcttggct ttatcatgct ggcaggtctc
     7141 attgtaaata atggaatcgt gctcattgat tacatcaatc aggctaggag agagggcatg
     7201 agcaagcatg aggcaatcat agacgcaggc cgtacaaggc ttcgtccaat actgatgaca
     7261 gcgatgacca ccatactcgc aatgtttaca tcagcgatag gtatcggaga tggctcagat
     7321 atgataaagc cgatggcaat cgcaataata ggaggtctcg tatacggaac catccttacg
     7381 cttatagtaa ttccatgtat ctatgatgcg ttcaacagag agaagagcat ggtagaggaa
     7441 gagctgtagc aagtagataa taatagctga catataagga tagagctgta caatcacagg
     7501 attaactaaa aaaccgctcg gaacgcaaaa aaagttccgg gtggtttttt ataataaaaa
     7561 gtagttttat ctgcttattt tttatggcta ttgcatattg cacaaaaaat gattaataaa
     7621 tttaaacaaa tagctgaaaa tgcttttgca acgttatata ctattgaaaa gtcaatgcta
     7681 tagtaatata atacaaacag aagccaagag aaaaacataa cggcttatat tttttacaac
     7741 aattaattaa caaatttaac taactatcaa ggaggaagca atcatgaagt attttctgga
     7801 cagtgcaaaa atcgaagaaa tcaaggaggc ctaccataca tttggtattg atggagtgac
     7861 aacaaatcca cgccacataa tggtaagcgg aaagcctttc cttactgtaa tcagtgagat
     7921 ggcagactgg gttaaggcgg aaggaatcga gggatacgag aaattcccta tttccgttga
     7981 aatcaaccca caccttgatg atgcagatga gatggtagca gaggcaagaa agatttcagc
     8041 tatctgcagc aactttgtta tcaagattcc gtgcacagag gccggtattc aggcagcaag
     8101 aagactcgag gcagagggaa tcagaacaaa tgtgacactc gtattctcac cagcacaggc
     8161 tattctgccg gcaaagaata attcgctttt tgtttcacct ttcatcggct ggaaagaggc
     8221 aaatggagag gattgtaaag cgtacattca ggctatttca gatatctatg acaattatgg
     8281 atataaggga aagacacaga ttatctgtgc ggctatccgt acacctaagc agattgtaga
     8341 ctgtgcggtt gcaggagcag atatcgttac aacaggcctt gcagtataca aggacagtat
     8401 caaacatgca tataccagac agggacttga gacattccag aatgcatggg ataatacagt
     8461 tacagaatag atgaaacagg gaggtgtttg taggcaatga aaattggatt tgtaggactc
     8521 ggaatcatgg gggagtccat gagtgagaat atagtgaaga agcatgatga tgaggtatat
     8581 gtatttgatt tcgtaaagga gaaggtagag ctattggaga gcaagggtgc aatcggctgc
     8641 agcagttcgc ttaagctggc agaaaaatca gacgtaatta tatcaatggt tccaaagtca
     8701 gagcattcga tggctgttta taaggaggtt ttaccggcac ttgatgatac aaagctctgt
     8761 attgatatga gcacgataga tccatctgtt tctgtggaga tttctgaaat ggtaaagaag
     8821 acgggcgcag agtttgtgga cgcaccggtt gtgaaatcga agcctgctgc tattgcagga
     8881 aagctcggaa tatatgtcgg tggagatgaa aaagcatgtg agaaggctat gccgattctt
     8941 tcctatatgg gagaaaatat catcaggatg ggtggaaacg gaaaaggtct ggtcatgaaa
     9001 atctgccata acgcgcttgt atcacagatt caaaatggtg taaatgagac actgacactt
     9061 gcaagggcat atggaataga tatagaaagc tatactacag ctatctcata tggcggtgga
     9121 cagaactttt atctggattc caaaaatgtg ccaatcaaaa atgaggatta caccactgcc
     9181 ttttcgattg aaaacatgta caaggatgtg aatatctgca tgaatatggc aaaggagagt
     9241 ggattaaagc ttcaaggaga aaacgctgca aagcaggtat atgacaaggc aatggaactt
     9301 ggctttggaa aagaagattt ctgcgctact ataaaggctg tcaaggcaat gcaaagataa
     9361 gtgggggaaa tatgttaaaa catttgaatg aaaaaaattc tgtgactata tacagtgtca
     9421 ctgatccatg ttttgaaacc tacggaaggg tgatttcaaa tatcaattgc aacgaactta
     9481 tctcatttat ggaacaaaat actgtaattc ctgagaaagg aaatatatat gtggcttcag
     9541 tagaagagct tgaacgtaca catatagcag agttttttaa aaatcgtata tatggagaga
     9601 tgcctataca gataggctac tgtaatggca gaaacaccac atacaatggt tttgagtacc
     9661 ataagggcag cgaaatcaat atagcggtaa ctgactttat gctggtgctg ggacatacct
     9721 gggagataaa agacaataca taccgtattg aagatgcaaa ggtatttttt gtggaaaagg
     9781 gtacagcaat agagatgtac cagacgacac ttcatctatc accatgcagg gtgtcagata
     9841 ttggatttaa ggatgttgtg attcttccaa ggggaaccaa tacaccactc aataatggag
     9901 ggacagatga agctgctata ggagaagaga gactgctttt gcaaagaaat aagtgggtaa
     9961 tagcacatcc cgatagagaa ccattgataa aacaaggggc atatcctgga ttgcttggag
    10021 agaataagga actttattat taaaaaaacc acgtgaccga aagactgaat actggacata
    10081 ttactgtact ttcggttgcg tgggaaataa ttttcagaaa aagtatatca tgtgagagat
    10141 tgaatgtgaa tagttagttt caacaaatcg atgtatattt tttctattaa attggaggat
    10201 tttatggagc atttagtatt tactttggtg acatttgtgc ttttcacagc actggttgga
    10261 attatttcag gaaagatagt gaagaatagc gatgaccaga aaacggcaaa gggttatttc
    10321 cttgccggaa atggacttgg aggttttttc attgcaggct caatgctgct tacgaatctg
    10381 tcggcagaaa atctggtagg tttgtcggga cagtcatatg cagctaatat gtcaggcatg
    10441 gcatgggaag caacagcggt tatagccaca attattatgg cttttgtgtt tttgccgatg
    10501 tatcttagaa aaggatatac aacgctgccg gagtttatgg aggagaggta tggcgttggt
    10561 gtacgccgta tggtttctat cctatttctt attggatatt tagtaatcgg aattcctgta
    10621 tgtctgtacg caggcgctat agcatttgag cagatttttg attttgctac agtatttgga
    10681 atcagcgagg gtatggcact tacaattctt atagtgcttg taggtattat tggtgcacta
    10741 tatgccgtac ttggaggtat gagagcagta gctgtatcag acacaatcaa tggaatttta
    10801 cttgttttag gctcagtgct tgtagtcgta tttggtttta tagctgttgg aaaattaagc
    10861 ggaggtggat ttattgtcgg tgtgaaggat gttattacat atgaaccggc aaagctcaat
    10921 gcgattggtg gaaagaatga tcctgtacca tttgcatgta tttttacagg tatgctgctt
    10981 gcaaatctgt tttactgggg tactaaccaa gtacttattc agagagcact tggtgctaaa
    11041 aacttaaagg aaggacaaaa gggagtttta ctttcaggct ttttaaagat gatggtaccg
    11101 ttacttcttg ttattcccgg agtaatcgca gcaagacttg taccaggtct tacaagcaag
    11161 gattttgcat atccttcact tgtagctagg tcattgccat ggccggttgt aggactgttt
    11221 tgtgcagcat tgtttggatc aattatctca acttataatt catttctcaa tgcagctagt
    11281 accgtattta tgattgatat ttataagccg atttttaacc caaatctctc agaggaagct
    11341 acagtaaaac ttgcaaagaa aattggatgg ggctttgcag catttgccat tatctttaca
    11401 ccattccttg atgtgcttag cacaggactt tatgatttcg gacgaagctt tacaggtttt
    11461 tataatattc ctatcattac aatggtgctt gtaggaatgt tctcaaagaa gggctctaag
    11521 cttggagcaa tatgtgcaac aatattccat attgcatttt acgcatgcta caagtttatc
    11581 ttcccacata ttgacacacc attcacaaat gctgttagtg gaattaatta tatgtatata
    11641 tacgctatct catttgttat catgcttgta atcatctttg tatgcagcaa gattgcacca
    11701 aatacaaagg tatttgacaa gagtgcttca agaaatgatg gctatgatat gacagcatgg
    11761 aagcataaaa atgcttttgc aatctggctt gtttcattcc tggtatatga gtatttcatt
    11821 ttctcgcctg caggaattgc aacagagaac aaaaatgtag taagaattgc tattgtaaca
    11881 gcaatatttg tgattgagac aattgttctg attgtgaaag agatgaacaa gcgtaaaaag
    11941 acagcttagg gaggttatta aatgcagatt aaagtaaatg ataatgaatt tcagcttttt
    12001 gtgggagaaa aaaggattct ggagcatagc aaagaaagac cgatgattta tgtcggagta
    12061 ggacaggaag atgttgatat gtacagaggt aacttcaaga taacagatta tgttaccgag
    12121 cgttttccac taaagctcac agatgtgatt cagacagctg atacagtcag actatgcttt
    12181 gaaagctata tcattgcgaa aattaaatgt gacgaaaatc tgtgtaccat tgattttgaa
    12241 caaaaggatg acaggattaa ccgattctgg tttagggtag ccgcggataa agaggaaaaa
    12301 tgctatggct gcggtgagca gatgtcttac tttaatctgc gtggaagaaa ttttcctatc
    12361 tggacatctg aaccaggtgt cggacgtgat aagacaactt acgttacatg gcgttcggat
    12421 gtagaaaata aagctggagg agactattac aacacaaatt atccacagcc aacctttgtt
    12481 tccacaaata aatattatct gcatgtggat tcaacagcat atgctgattt tgatttcaga
    12541 aatgacagct ttcatgagct gcagatatgg gaggtgccta agcagatccg catagagtgt
    12601 gctgatacat atctcaaact gcttgagaga atcacaacat attttggaag acagccaaag
    12661 ctcccggatt gggtatacaa tggtctcata atcggtgttc agggtggaaa tgaaaggtca
    12721 tttggattgc ttgataagac ccttgatagg aacatcaagg tagctggcat atggtgccag
    12781 gattggtgcg gaaagagagt gacatcattc ggaaagagac tccaatggga ctggaaatac
    12841 cacaaggaaa tgtatccgga tctgccaaag aagattaaag agataaatgc aaagggcata
    12901 aaatttttgg gatatgttaa tccatatctg gtaaatgatg gtgagctcta taaggaaggc
    12961 aaggaaaaag gctattttgc aacaaaggca gatggcagtg attatctcgt ggattttggt
    13021 gaattttatt gtggagttgt ggatctcaca aatccggagg cattcgagtg gtttaaggat
    13081 attattaaag aatacacact tggtataggc attgatggct ggatggcaga ctttggagag
    13141 tatctgccta cagatgatat atgcttatac agcggtaaat cacctatgat tgagcacaat
    13201 cactggccgg tgctatgggc aaagtgtaac tatgaggcag taaaagagtc aggaaagctt
    13261 ggagatgtgg tttacttcat gcgtgccgga ggtgcaggaa gccagaaata ctgcacattg
    13321 ctgtgggccg gcgatcagtc tgttgacttt acaatacatg atggactcgc atctgttatc
    13381 tgcggagcac ttagtgcagg aatgatggga tgtgggctca cgcatagtga tattggaggc
    13441 tatacatcgc tttttgataa tactcgtaca aaggagctgt ttctcagatg ggctgagatg
    13501 gcgatgttta caccatttat gaggacacat gagggaaaca gaccggatac caattttcag
    13561 tattacgatg atgaggatac catggagcgg ctggcgagac tcgttgatgt atacacgatg
    13621 cttgcaccgt atacaaagac acttgttgaa gagaatgcag acagtggaca tccggtgcaa
    13681 agacctttgt tcatgcatta tgagtctgat gcgaaagctt atgacataca gtatgagtat
    13741 ctttttggaa gagatatgct gatagcacct gtttatgagc aggataagca tgaatgggat
    13801 gtgtatctgc cacaggatga atgggtacat ttgtggacag gcgaagagta tcacggcgga
    13861 gaaataacag tatcagccga gcttggctat acacctgcct tctacagaaa aaatagtgag
    13921 tttgctgata tatttgaaga aatcagagaa aaatatggag tcaaataaat gaacactgtt
    13981 ttattttaca taataatttt tctgacaaat gtgattcagg gcattacagg ctttgcgggg
    14041 accatcctcg caatgcctcc tagcctaatt ctggtgggat atgatgtcgc taagccgata
    14101 ttaaatgtac ttggtattct tgccggagtg tatgtctttg ttacacaatg gaaacatgta
    14161 gactggaagc agttgaaaaa ggtagtcatc gtcatggcta taggaatctt tggcggcttt
    14221 tttctaaaga acgcacttgc aggatatgat gcagtattat acaagtttct gggtattttt
    14281 gtgatatttc ttgcaataca tgggttttac agcatatatg ggcaaaggca gcgtgataca
    14341 aaagaaaaat catcatggaa atcaatgctg ttacttattg gtgcgggaat agttcatggt
    14401 atgtttgtgt caggcggacc gcttctgata gggtatttat ccaatgagat aaaggaaaag
    14461 acaagcttta gagcaactat ttctactgtt tggattatat taaattcgat gatacttgtt
    14521 caggatatat gcgcgggaat gtggaatgca gggcttgtta tgactcaatg catatcgatt
    14581 ccgtttttga tagcgggaat ggcagcaggc tcagtgcttg taaagaggat gagccaggaa
    14641 atatttatga agctcaccta catattactt tttatatcag gaatactgct tatggttaag
    14701 tagtttttga aaattttatt ggaggtaaaa tatatgacaa aatttcaggt ggcatatgtg
    14761 ccggtcgggg ttccgacatt tcatttggag agcgcacaga aggaatttga aaaatcaata
    14821 gacttattaa atacaataac ggatgctgca attgtaccag ataaaatgct tttatcaatt
    14881 caggatttaa ctaattttct tgatacaata gagccatccc tgataatttt acagaatata
    14941 acctttgcaa atgcagctta tgcgagtgag gttcttaaaa gattttcgtg tccggtattg
    15001 ctttggacac ttcgtgagcc ggttatagat ggaggccggt tgagattaaa ttcattgacg
    15061 ggagcatatt cggcaggaaa tgcactggct gcgtttaggg atggtaactt tgaatatatc
    15121 tttggagctc cacaggagga gaggacaaag aaaacaatta gtgcctgtat tcgtgcagca
    15181 tggcttaaaa atgagcttaa aaatctaagg attgcctcag tcggacatac acctcaggga
    15241 tttggatttg gacgtgcaat ggatgctgac atgcaaaagg tattcggagc aacacttgag
    15301 tcaattgagg tgagagagct tattgatatg gcaaagggat atactgatga tgagtgcagt
    15361 gaatatctcg ataaaatgac taagtgtact gttggtcttg acaagactcc tgacaataat
    15421 cgaaaggatt ttgcgagact gtgcaaggcc tataaggagt atgtagatga aaataatatt
    15481 ggagcacttt cttcaagatg ttggcctgat ttctttaccg cgtttggaac acctgtttgt
    15541 ggcgtgcttg ctatgctaaa tgatatgggg gttgctgcaa gctgcgaggc tgatacatat
    15601 ggagcactct ctatgtatat gggaatgaaa ttatctgagc aggctgtatt tttcggagat
    15661 ccggtgtctc ttgatgagag tgaaaataca attacattct ggcattgcgg aacagctgca
    15721 tgctctatcg caagagagga cacaggtgca acaatcggag tgcatccgaa cagaaagatt
    15781 ggccctgtaa tggactttgg ctgtaaggcg gctgacaaag tcacagtgtt cagagtagga
    15841 caaaaagcag atggaagctt tagatttttt gtcacaacag gaagtgctct cgacaagcct
    15901 aagcagtttt gcggaacatc aattgtagtc aggacagaca aaaatagcta tgatattgta
    15961 aatcaaagtg taaaggatgg ttgggagcct cattttgtgg tgatttacgg agattgttca
    16021 gatgagttaa aggttttagg acatatgctt gaaatcgaag tctgtgagta ctgattttat
    16081 ggaaatagct taaatatcat gctataataa tagtagtatt taggtaccga ataattggtt
    16141 ggaggatttt tacatggcaa agtatattat ggcattagat gcgggcacga ccagcaacag
    16201 atgtatactt ttcaatgaga agggcgagat gtgcagcgtg gcacagaagg aatttacgca
    16261 gtttttcccg aagccgggtt gggtggagca cgatgcagag gaaatctggg ccacacagct
    16321 tgaggtggca aaggaggcaa tggcaaatat acaggctacg gcagccgaca tatgtgcgat
    16381 aggcattact aaccagagag agaccacgat tgtgtgggac aaaaatacgg gagagccggt
    16441 ttatcacgca atagtatggc agtgcaggcg cacggcagag tatgcggatt cactgaagga
    16501 aaaggggctt accgagacct tcaggaaaaa gacaggtctt gtgatagatg cttatttttc
    16561 ggcaactaag ctcaagtggc tccttgacaa tgtgccggga gcaagggaga gagccgaaag
    16621 aggcgagctt ttgtttggaa cagtcgagac atggattata tggaagctca ctcaggggca
    16681 ggtgcatgtt acggattatt ccaatgcatc aagaaccatg atgtttaata tcaatacctt
    16741 aaagtgggat gatgagatac ttaaggagct tgacatacca aagagcatgc ttccaaagcc
    16801 tatgccgtca agctgtgtgt atggtgaggt gaatccggtg tacttcgggg gaccgattcc
    16861 tatcgcggga gcggcaggag accagcaggc agcgcttttt ggtcagacct gctttagggc
    16921 aggagaagcc aaaaatacat acggtacagg ttgttttctg ctgatgaata caggtgagat
    16981 gcctgtttca tcaaagaacg gacttgttac cacaatagcc tggggcatag acggcaaggt
    17041 ggtatatgcg ctggagggct caatatttgt ggcaggagcc tcaatacagt ggctccgcga
    17101 tgagatgaaa tttatcgatt cctcaactga ttcagagtac atggcgagaa aggtaaagga
    17161 tacaaacggc tgttatgtgg tgccggcatt tacagggctt ggggcaccgt actgggatca
    17221 gtatgcgaga ggaactattg tgggtcttac gagaggtgta aacaagtacc atgtcataag
    17281 ggccactctg gaatcaatgg catttcaggt aaatgatgtg cttgaggcaa tgaaggctga
    17341 ttcgggcatc aatctcacct cgcttag
//
LOCUS       DPUF01000210            4053 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16032, whole
            genome shotgun sequence.
ACCESSION   DPUF01000210 DPUF01000000
VERSION     DPUF01000210.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16032"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="DHV81_07505"
     CDS             complement(<1..1108)
                     /locus_tag="DHV81_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94297.1"
                     /translation="MSMTETIKLYDRDAYAIEFEADIISCEPNKADDKQFDIILNQTL
                     FFPEEGGQSPDMGILGGYKIVDVQIKNGVITHTVDISADDCCIMGKEEAQTEKTTDGQ
                     IIGMECVSEKVELAAGVHVQGKIDWQHRFYNMQQHSGEHIFSGIVHRRFGFENVGFHL
                     SDSVVTMDFSGVISPEDIAEVEHEVNVAISKNIPIEVTYPSRDELAQLEYRSKIEIEG
                     QVRIVTVPGYDVCACCAPHVKRTGEIGMLKVMNYQNYKGGVRISIFCGFRALEAFRQK
                     CDIISELMGIFTTNQEAIVDNVTKLKAVNQSLKSELGTAKSALLDYKVAELPADTDNA
                     VLFEDGIDTNTARNCVNGLVEKYSGFSAFFTGNDE"
     gene            complement(1190..3280)
                     /locus_tag="DHV81_07510"
                     /pseudo
     CDS             complement(1190..3280)
                     /locus_tag="DHV81_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743832.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     assembly_gap    1766..1826
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3273..4040)
                     /locus_tag="DHV81_07515"
     CDS             complement(3273..4040)
                     /locus_tag="DHV81_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI94298.1"
                     /translation="MAILEVSNLKKIYTTRFGGNQVQALKNINFSVEEGEYVAIMGES
                     GSGKTTLLNILAALDKPTAGEVLLNGKNLVSLKEKEISAFRREHLGFVFQDFNLLDNF
                     SLKDNIFLPLVLSGVDYRDMEKRLAPIASLLGIEKLLNKYPYEVSGGQKQRAAVARAI
                     ITNPELILADEPTGALDSKATDSLLRLFNRINEDGQTILMVTHSTKAASHANRVLFIK
                     DGQVFHQVYRGNLTNDELYVKIQDALMLITTGGEKHE"
BASE COUNT         1118 a         1018 c          651 g         1205 t
ORIGIN      
        1 cctcatcatt tcctgtaaaa aatgcactaa agccactata tttttccaca aggccattga
       61 cgcaatttct tgctgtattc gtatcaatgc cgtcctcaaa cagtactgca ttgtccgtat
      121 cagcaggaag ctctgctacc ttataatcca aaagagctga ctttgcagtg ccaagctctg
      181 acttaagtga ctggtttact gccttaagct ttgtgacgtt atccacaata gcctcctgat
      241 ttgtggtaaa tatccccatg agctctgaga tgatgtcaca cttttgtctg aaagcctcca
      301 gtgccctgaa accacaaaag atgctgattc tgacgcctcc tttataattc tgataattca
      361 taaccttaag catgccaatc tcgccggtgc gcttcacatg cggcgcacag catgcacaca
      421 catcataccc cggcactgtc actattctga cctgtccttc aatctcgatt ttgcttctgt
      481 actccagctg cgcaagctca tcccttgacg gatatgtcac ctctatcgga atattttttg
      541 aaatggctac atttacctca tgctccacct ctgctatatc ctccggtgaa atcacgccac
      601 tgaaatccat tgtcactaca gaatcactca gatgaaagcc cacattctca aaaccaaacc
      661 tgcggtggac aattcccgaa aaaatgtgct ctccactgtg ctgctgcata ttgtaaaaac
      721 gatgctgcca gtctattttg ccctgtacat ggactcctgc tgcaagctcc actttctcag
      781 aaacgcactc catacctata atctgtccat cagtcgtctt ttctgtctga gcctcttctt
      841 ttcccattat gcagcaatca tctgcggaaa tatccaccgt atgagtaatg actccattct
      901 taatctgcac atcaacgatc ttatagcctc caagtattcc catgtccggg ctttgtccgc
      961 cctcctccgg gaaaaataaa gtctgattta atattatatc aaactgctta tcatctgcct
     1021 tgttcggttc gcatgatata atatctgcct caaactctat ggcatatgca tccctgtcgt
     1081 atagtttaat tgtttctgtc atactcatac tgcttgttcc tctcgctcta tattcttatg
     1141 catcattgct atgtcctatt atagcgcatt cataattcat ataaaacccc taaatctggt
     1201 ttcccacaat cctgtagtat gctctcgaag taatcttgaa aacaatatag tatatgacca
     1261 taaacacaaa aagcgtagca accatacaga tcacaataag cttaacatcc gtcagattga
     1321 acatgacaag cagtctgcga agcagaggaa atgctgctgc aagatgacac gctgcaagga
     1381 taatcggcag gaaaaacacc atacgcacct gcgaagtaat ggatttcttc acctcgtcat
     1441 tgctcatgcc caccttctcc ataatctcat agcgtgcttt gtcatcatag ccctctgaaa
     1501 tctgcttata gaaaataatc atcacagtaa ccataagaaa catcgttcca aggaatattc
     1561 cgataaaaaa caggccacca tacaatacat aaatctgttc tctgtttgca tcacggcttt
     1621 cctcatagaa gcctgacaca tctgatttta cactattttc ctcaaaatgc ttatccagag
     1681 cctttgccac agcaagctta tcctcactgc tgccatacag attataatca atttcattgt
     1741 tgagcatagt cggcacacta tcctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnctgt tcaggttaaa tatctctgca agtgttgcct
     1861 tgctgtctac cactacatag tacacaccac tatacataga ggacatatag ctgtctttac
     1921 ttacagcagt tttttccgcc gcagccactt taaattcttt gcccaacaga ttaattatat
     1981 cgccattaaa cttttctgtt ccataaacag ccaccgaacc ggtatcaagc tgtgggattt
     2041 ttttattcag actcttttta aagccggcct ccatattatc aatttcatct cctgtgacaa
     2101 aatacaacag ggaaatatcg ttatcgaaac ttaacaccgg cttaaactct gttccctctc
     2161 taaacactgt ccagctaaaa taagtgtagc ttttgctgtc tctaacatta caagcagagt
     2221 cagctattat tttatccgaa tctgcagcag cttctttaag tgcatcatcc agattcacat
     2281 catccggcac ttccttatac cagctatata cactgatatc cgaaggatat ctctgcttaa
     2341 gttctccctc catgcccgca tacatgctca cggttgtgga aacaacaacc aacaccattg
     2401 tggaaagaac gcatatactc gctagtccgc ccgcattctg cttcattctg taaatcattc
     2461 ctgaaaccgc cgcaaaatgc tttttattat agtaaaactt cttgtttttc ctgagtgcct
     2521 taagtatcac aatgcttcct gaaataaata aaagatacgt tccgatgatg acaagcagca
     2581 ctgcgacaaa gaacagtgaa agcacctgaa gagggctctt tgtggtaatc gcaatataat
     2641 atcccccggc aagacagcct agtccgatta tagcgataag ccatttgctc ttcggctctt
     2701 tctcaccgac attgccaccg cgaagcagct ctatcggatt agcaagcttc acctgcatca
     2761 ggttatagat gagagtaaga aaatacagca ctccaaaaat aagtattgta tttacgacag
     2821 cgccagtaga tacagcgaaa tcaatttgtg ctttaatatt tataatccta taaagaaaca
     2881 taatcatcag cttggaaaat aaaataccgg ctatgattcc agatactatt gctgcgagtg
     2941 ctacggtaag tgtctcaatt atcaaaactc tcgcgatatg acgcttttcc atgccaagaa
     3001 tattgtatat tcctacctct ttcttgcgcc ttttgatgat aaagctgttt gtataaaaca
     3061 gaaatatata tgaaaaaacc gctattgttc cgcatccaag ccccattatc gacgccagtg
     3121 catccgcgcc cggcaccttg ccaagtccct ttgagttgtt taaaaacatc atcacataaa
     3181 acatggctat catgaccatt cccgatataa tgtaaggcat ataaagagtc ttattatttt
     3241 ttatatttgt aaatgccagc ctgctatata ttttattcat gcttctcacc acctgtcgta
     3301 atcagcataa gtgcatcctg aatcttcaca tagagctcgt catttgtaag attgccacgg
     3361 taaacctgat gaaacacctg accgtccttt atgaaaagca ccctgttggc atgacttgca
     3421 gcctttgtgc tgtgtgtcac catgagaatg gtctgtccgt cctcgtttat cctgttgaac
     3481 agcctaagca gactgtctgt tgcctttgaa tccagtgctc ctgttggctc atctgccaaa
     3541 attaattcgg gatttgttat tatcgcacgt gccactgctg ccctttgctt ctgtcctcct
     3601 gacacctcat atggatattt attaagcagc ttttcaattc caagcagcga agctataggt
     3661 gcaagccttt tttccatatc cctataatcc acacctgaca gcacaagcgg taaaaagata
     3721 ttgtccttca gtgaaaaatt gtcaagcaga ttaaaatcct gaaatacgaa gcccagatgc
     3781 tcacgtctga aggcagatat ctccttttcc ttcaatgaga caaggttctt tccatttaag
     3841 agtacctctc ccgctgtcgg cttatcaagt gccgcgagaa tatttaaaag tgtcgtcttt
     3901 cctgagccgg actctcccat gattgccaca tactcacctt cctctacaga gaaattgatg
     3961 tttttcaatg cctgtacctg gttgccacca aatctggtgg tatatatttt tttgaggtta
     4021 cttacttcta agattgccat ttcaatgctc ctt
//
LOCUS       DPUF01000211            3071 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_25192, whole
            genome shotgun sequence.
ACCESSION   DPUF01000211 DPUF01000000
VERSION     DPUF01000211.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_25192"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="DHV81_07520"
     CDS             complement(<1..1736)
                     /locus_tag="DHV81_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCI94299.1"
                     /translation="MKIIKRSGSEDIFDAAKIVAAIRKANDSVLDYEKLTADQIEEIA
                     SNVETACENMKRSPSVEEIQDMVENQLMNKRAFTVARNYITYRYKRAIVRKSNSTDQQ
                     ILSLLECNNEEVKQENSNKNPTVNSVQRDYMAGEVSKDITRRFLLPEDIVEAHEKGLI
                     HFHDADYFAQHMHNCCLVNLEDMLQNGTVISETMIDTPKSFSTACNIATQAIAQIASS
                     QYGGQSISLAHLAPFVQVSREKFRKQVQKEFESVGLDLDEEKINEVAELRVKEEINRG
                     VQMIQYQVITLMTTNGQAPFITVFMYLNEVSDPQTKADLAAIIEEMLHQRIKGVKNEK
                     GVYITPAFPKLIYVLEDDNITEDSKYWDLTVLAAQCSAKRMVPDYISEKVMKELKDGD
                     VYTCMGCRSFLTVDRCSQTAGNVAQAKNFDPNKRKYYGRFNQGVVTLNLVDVACSSEK
                     DEEKFWKILDERLELCHRALMCRHKRLLGTKSDVAPILWQNGALARLKKGEVIDPLLF
                     NGYSTISLGYAGLCECVRYMKGVSHTEPKEGTPFALKVMQRLNDACEQWKKESNIDFS
                     IYGTPLESTTYK"
     gene            complement(2122..2574)
                     /locus_tag="DHV81_07525"
     CDS             complement(2122..2574)
                     /locus_tag="DHV81_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCI94300.1"
                     /translation="MKEFTPDKYLGHKINRISRSIDRYFDRKRCSKTECIPRSQGMMI
                     GYIMDNQGQNIFQKDIEAKFHLSGATVTNMLKSLEKNGYIVRTPMENDARLKRIELTQ
                     KALDHENRVRENIMVIEEAMHEGFSKEEFNMMLGFLDRVIDNMEKLNK"
     gene            2743..>3071
                     /locus_tag="DHV81_07530"
     CDS             2743..>3071
                     /locus_tag="DHV81_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI94301.1"
                     /translation="MLKTLGAQIKEYKWASIATPVFMLLEVAVDTIIPLLMASIIDNG
                     VNKGDTKHIYIMGVWMIVAALFGLLTGCLGAKYGAKAAMGFGKNLRAAMFRNIQTFSF
                     ANIDRFSS"
BASE COUNT          792 a          700 c          564 g         1015 t
ORIGIN      
        1 aacttatagg tggtagactc aagcggtgtg ccatatatac tgaaatcgat gtttgactcc
       61 tttttccact gctcacatgc atcgtttaag cgctgcatta ccttaagtgc aaacggtgtt
      121 ccctcttttg gctctgtgtg tgatactccc ttcatataac gtacgcactc acaaagtcct
      181 gcatagccga gtgagattgt tgagtatccg ttaaataata atgggtctat aacctctccc
      241 tttttcagac gtgcaagtgc tccgttctgc cagagaatcg gtgctacatc ggatttggta
      301 ccaagaagcc tcttatgtct gcacataagt gctctgtgac acagctcaag tctctcatca
      361 agtatcttcc agaatttttc ctcatccttt tctgatgagc atgccacatc aacgagattt
      421 aaggtaacaa caccctggtt gaaacgtcca tagtatttac gcttgtttgg atcaaagttc
      481 tttgcctgtg ccacattgcc cgctgtctgt gagcatctgt ccactgtgag gaatgaacgg
      541 cagcccatgc atgtatacac atctccgtcc ttaagctcct tcatgacctt ttctgaaata
      601 tagtcaggca ccattctctt tgctgagcac tgtgcggcga gcactgtgag atcccagtac
      661 tttgaatcct ctgtgatatt atcatcctca agcacatata tgagcttagg gaatgccggt
      721 gtaatatata cacccttttc gttctttacg ccctttattc tctggtgcag catctcctca
      781 atgattgcgg caagatctgc ctttgtctgc ggatcggata cctcattcag gtacatgaat
      841 actgtgataa acggtgcctg tccgtttgta gtcatgagtg tgatgacctg atactgaatc
      901 atctgcacac cgcggttgat ttcttccttg acacgaagct ctgctacctc gttaattttt
      961 tcttcatcca aatcaaggcc tacgctctcg aattcctttt gcacctgctt tctgaacttc
     1021 tcacgactga cctgtacaaa tggtgccaga tgtgcaagcg agatactctg tccaccatac
     1081 tgtgagctgg caatctgtgc gatagcctgt gtagcgatgt tgcaggctgt tgaaaagctc
     1141 tttggtgtgt caatcatagt ctcactgatt actgtgccgt tctgaagcat gtcctcaagg
     1201 tttacaagac agcagttgtg catatgctgt gcaaaataat ctgcatcgtg gaaatggata
     1261 agccccttct catgagcctc cactatatcc tcaggaagca gaaaacggcg tgtgatatcc
     1321 ttgctcacct cacctgccat gtagtcacgc tgcacgctgt ttacagtagg attcttgttg
     1381 gagttttcct gctttacctc ctcgttattg cactcaagaa ggctaagaat ctgctggtcc
     1441 gtagagtttg acttacggac gatggctctc ttatatctgt aagtaatgta gtttctggct
     1501 acggtaaagg cacgcttgtt catgagctgg ttctccacca tatcctgtat ctcctcaacg
     1561 cttggagagc gcttcatgtt ctcacatgca gtctccacgt tgcttgcaat ttcctcaatc
     1621 tgatcagcag taagcttctc atagtctagc actgaatcgt ttgcctttct aatggcagct
     1681 acaattttgg ctgcatcaaa tatatcctca ctgccgcttc gcttgatgat tttcatgtaa
     1741 atttctcctc tctgtatact atattccata taatgtagtg tactatatat agtattgtca
     1801 ttcaatgcac atactatata tatacatttt atcatgtgta atggcacaaa atatgcagct
     1861 ataaatgaga tgtaatcatt aattcatgtc actgccctgc aatgtaaagc gggcttttca
     1921 ccaaacaatt acgatatagc tacatataaa atgccatatt atttaataca aatctgtgca
     1981 tctgtctttt gatgacaatc taaattatac aagatattgt tgctatgtca atacgctcaa
     2041 caatattttg tagcattttg acttattgca caaaaaaaaa cacaatattt atgctacttt
     2101 ttcaactctg taattatttt gttacttgtt aagcttttcc atattgtcaa tcactctgtc
     2161 caaaaagcca agcatcatgt taaactcctc cttagaaaag ccttcgtgca tggcttcctc
     2221 tattaccatt atattttccc ttaccctgtt ttcgtggtcc agtgcttttt gcgtcagctc
     2281 aatcctctta agccttgcat cattttccat aggtgtgcgc acgatatagc cgtttttttc
     2341 aagactttta agcatatttg tcactgtggc tcctgacaga tgaaactttg cttcgatatc
     2401 cttttgaaat atattttggc cctgattatc cattatatat ccaatcatca taccctggct
     2461 tcgcggtata cattctgttt tactgcacct ttttctgtca aaatatctgt ctatactcct
     2521 gctgatgcgg tttatcttgt gtcctaagta tttatccggt gtaaactcct tcatatctga
     2581 aatcctttca tagatttttc ttgacaataa aaaaagtata tctataatat agttagcaca
     2641 ctaactaatt aaatgcaagt attttttagt taaccacatt accataaatg tgggaagttt
     2701 gttttaatca aatataaaat ataagaagaa aggacaaaac atatgttaaa aacactaggt
     2761 gcccaaataa aggaatacaa atgggcttcg attgcgacac ctgttttcat gctgcttgag
     2821 gtggctgtgg ataccatcat cccccttctc atggcatcaa tcatagacaa cggtgtcaat
     2881 aagggcgata caaagcatat ctatatcatg ggtgtgtgga tgattgtcgc agcactgttt
     2941 ggtcttctga caggatgtct cggtgcaaaa tatggtgcaa aggccgccat gggctttgga
     3001 aagaatctgc gtgctgcaat gtttcgcaac atccagacct tttcctttgc aaacatcgac
     3061 agattctcat c
//
LOCUS       DPUF01000212            2682 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7012, whole
            genome shotgun sequence.
ACCESSION   DPUF01000212 DPUF01000000
VERSION     DPUF01000212.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7012"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="DHV81_07535"
     CDS             complement(<1..1145)
                     /locus_tag="DHV81_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI94302.1"
                     /translation="MSKIFKNLIPYWRWIILIFVFLIAQAYCDLALPAYTSDIIDVGI
                     QDHGIEHILPEEITSEDYQMAQTFMLSDEKEKFTDCYEAEDASKSDDSVAKYKLTADS
                     DTLDKLDEELLVPLVMAYQAFQSREQMDVDASAIPQGVALDDTMIKQIRSKVQDKIDE
                     VGSATLKSMGVTFATKCDENAGIDVDANQVAYLWKAGAKMAAFAAIMLIAACVVGFAA
                     SKVGAAVGRDLREKTFSKVVGFSNAEINKFSTASLITRSTNDIQQIQMVTAMMLRMVL
                     YAPIVGIGGIIKVAQTKSGMEWVIAIAVAAIMVFIFTLVGIAMPKFKIMQSLVDKVNL
                     VSREILTGLSVIRAFGREKEEEKRFDEANRELTRTQLFTNRVMTFMMP"
     gene            complement(1369..>2682)
                     /locus_tag="DHV81_07540"
     CDS             complement(1369..>2682)
                     /locus_tag="DHV81_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5057 domain-containing protein"
                     /protein_id="HCI94303.1"
                     /translation="VLFAHDTTSFCNNKNDTRENNVWGYKFNSIIRDTVGLDRYGILS
                     NEYLKKGNTLKSTDEDFDKAVQTAKKNNTDIAYEPKSDNSVIVRQNQGFTYADLNQYQ
                     WKDNGNDHGEYRLYSGLDGNDVEAKTNKAVKVNSGQITTYPFKIADEINIATTHKQYY
                     QLDMNQDADGDGESDIVVWYTLSNHGIYEQSPKDVRNNYYIYTMGNVTYSGVGHSDFS
                     NNEDELKLYINTMIAAYSASVHEPSIYLKENADTDSNDITTLYATIDDAIEEEAANKA
                     DARLDGADSTQDVYFTVKDSNLVRNQIDEKTVVYLDFYIEDPQKNPKDETIGTGNDKI
                     YLKKVDWDIYTLNNDGTENEQINNSKENSQLNKDPRDFFENNKTYKVKVPLSALPEGA
                     NSIKIYAVGYSKIYQSQVSGGDKETTTAKAYKTFQIQRVGLADLD"
BASE COUNT          770 a          630 c          433 g          849 t
ORIGIN      
        1 ggcatcataa aggtcatgac acggtttgta aacagctgtg tgcgtgtaag ctcacggtta
       61 gcctcatcaa acctcttttc ctcctccttc tcacggccga aagccctgat aacggaaaga
      121 cctgtaagaa tctcacgaga aacaaggttt accttatcta caagactctg cataatcttg
      181 aactttggca tcgcaatgcc aaccagtgtg aaaataaaaa ccatgatggc agcaaccgca
      241 atggcaatca cccactccat gccggatttg gtctgcgcca ccttgatgat accaccgata
      301 ccaacgattg gcgcgtaaag caccatacga agcatcatag ccgtaaccat ctgaatctgc
      361 tgtatatcgt ttgtgcttct cgtaatcagt gatgccgtag agaatttgtt aatctcggca
      421 tttgaaaaac ctacaacctt tgagaaggtc ttctccctca aatccctgcc gacagcggca
      481 ccgacctttg aagccgcaaa gcctacaaca catgctgcta taagcataat agctgcaaag
      541 gctgccatct tggcgcctgc cttccacaga taagcaacct gattggcatc cacgtcgatt
      601 cctgcattct catcacactt tgtcgcaaaa gttacaccca tggactttaa agtagctgaa
      661 cccacctcat cgattttgtc ctgcaccttg cttcttatct gctttatcat ggtatcatcg
      721 agtgctactc cctgcgggat agccgatgca tccacatcca tctgctccct cgactgaaaa
      781 gcctgatatg ccatgacaag aggaaccaaa agctcctcat ccagcttatc aagtgtgtcc
      841 gaatcagctg tcagcttgta ttttgccaca ctgtcatcag attttgaagc atcctctgcc
      901 tcataacagt ccgtaaattt ttctttctcg tcagacagca taaatgtctg tgccatctga
      961 taatcctccg atgtaatctc ctctggcagg atatgctcaa tgccatgatc ctggataccc
     1021 acatcaatga tgtcagatgt gtacgcaggc aaagccagat cacaataagc ctgagcaatg
     1081 agaaaaacaa aaattaatat gatccatcgc cagtatggaa tcaggttttt gaaaatttta
     1141 ctcataaacg tttcctttct ttccggttaa ttatccccca tacacgaatg caaaaatatc
     1201 cgcaatcgtg cgcatactat ctattttatc aataagcgcg gataattcaa taaagaaagt
     1261 ataaagaaac agtaaaaaaa cagagactat ataaatggtt tatataagtc tctgtttgat
     1321 aaattgtgct taatcgaaaa gtatgtgata gccaggagcc tttgaaaact aatccaaatc
     1381 agcaagacct acacgctgta tctggaaggt cttgtatgcc tttgctgttg tagtttcttt
     1441 atcaccaccg ctcacctgtg actggtaaat cttactatat cctacggcat aaatctttat
     1501 agaattagct ccctccggta aagcactcaa tggtaccttt accttatatg tcttgttatt
     1561 ttcaaagaag tcccttggat ccttatttaa ttgactgttt tctttactgt tatttatctg
     1621 ttcattttct gttccatcat tattcagtgt atagatatcc cagtccacct tctttaagta
     1681 aatcttatca tttccggtgc caatcgtttc atcttttgga tttttttgcg gatcctcgat
     1741 ataaaagtcc aaataaacaa cagttttttc atcaatctga tttcgcacaa ggtttgaatc
     1801 ctttaccgta aaataaacat cctgcgtact gtctgcacca tctagtctgg catccgcttt
     1861 attagctgct tcttcttcaa tggcatcatc aatagttgca tataatgtag ttatgtcatt
     1921 tgaatcagta tctgcattct ctttaagata aattgacggc tcatgaactg atgcactata
     1981 tgcagcaatc atagtgttta tataaagttt caattcatcc tcattatttg agaaatcgga
     2041 atgtcctaca cctgagtatg ttacatttcc cattgtgtaa atataataat tatttcttac
     2101 atcctttgga gactgctcat aaattccatg gtttgacaag gtataccata caacaatatc
     2161 actctcacca tcaccatcag catcctgatt catatccagc tggtagtact gcttatgagt
     2221 ggtagctata tttatttcgt cagcaatctt aaaaggatat gtggtaatct gtcctgaatt
     2281 aaccttgact gccttattag tcttagcttc aacatcatta ccatctagcc ctgaatacag
     2341 cctgtactcg ccatgatcgt taccattatc tttccactga tactgattta aatctgcata
     2401 agtaaatccc tgattctgtc tgacaataac tgaattatca gattttggct catatgcaat
     2461 atcagtatta ttctttttgg cagtctgtac agctttatca aaatcctcat cagtactctt
     2521 taatgtattg cccttcttta aatactcatt tgacaggatt ccatacctgt ccagtccgac
     2581 agtatcacga attatagagt taaacttata tccccagaca ttattctctc gcgtatcatt
     2641 tttattatta caaaatgatg ttgtatcatg agcaaacaat ac
//
LOCUS       DPUF01000213           12745 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12008, whole
            genome shotgun sequence.
ACCESSION   DPUF01000213 DPUF01000000
VERSION     DPUF01000213.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 12745)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12745)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12745
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12008"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            105..>685
                     /locus_tag="DHV81_07545"
     CDS             105..>685
                     /locus_tag="DHV81_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI94304.1"
                     /translation="MVLSEITMEAVAALKANGHRAFICTGRARASVRSEKLLNMGFDG
                     IIAACGNYIEMDGKVIYENDLSADMVQKVIRVLSECKMPVVLEGSTDYWIDDEGFEND
                     PYVDYLFESLGEHAHIIRGYDGEIRINKFSADVIDGTDYERVKAEFADDFLILEHVEN
                     VVEFVPKGTSKATGIKWLCNHLDIPLDETYAIG"
     assembly_gap    686..826
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            932..1216
                     /locus_tag="DHV81_07550"
     CDS             932..1216
                     /locus_tag="DHV81_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCI94305.1"
                     /translation="MKLVPLSDRVVLKQLEAEETTKSGIILTSSAQEKPQEAEVIAVG
                     PGGMVDGKEVKMQVTVGQKVIYSKYAGTEVKLDGEEYIIVRQNDILAVVE"
     gene            1333..2958
                     /gene="groL"
                     /locus_tag="DHV81_07555"
     CDS             1333..2958
                     /gene="groL"
                     /locus_tag="DHV81_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCI94306.1"
                     /translation="MAKEIKYGSEARAALGAGVDKLANTVRVTLGPKGRNVVLDKSYG
                     APLITNDGVTIAKEVELEDAFENMGAQLVKEVATKTNDVAGDGTTTATVLTQAMVQEG
                     MKNLEAGANPIVLRRGMKRATDKAVEALKDMSQKVKGKEQIAKVAAISAGDEEVGNLV
                     ADAMEKVTNDGVITIEESKTMQTELDLVEGMQFDRGYLSAYMCTDMDKMEAVLDDPYI
                     LITDKKISNIQDILPLLEKIVQAGARLLIIAEDVEGEALTTLIVNKLRGTFNVVAVKA
                     PGYGDRRKAMLEDIAVLTGGQVISSDLGLELKDTTIDMLGRAKSVKVQKENTVIVDGA
                     GDKDAIAGRVSQIRGQIDETTSEFDKEKLQERLAKMAGGVAVIRVGAATETEMKEAKL
                     RMEDALNATRAAVEEGIIAGGGSAYIHASKKVAELVDTLEGDEKTGAKVILKALEAPL
                     YYIAANAGLEGAVIINKVKESAPGTGFNAATEEYVDMVDNGILDPVKVTRSALQNATS
                     VASTLLTTESAVATIKEDTPAMPAGAGAGMGMM"
     gene            3298..3552
                     /locus_tag="DHV81_07560"
     CDS             3298..3552
                     /locus_tag="DHV81_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005375341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HCI94307.1"
                     /translation="MPNIKPISDLRNYTDVLKEVDISSRVYLTRNGRGEYGILTMEEI
                     DELDRLKAIYTLNKKLQEAEDEAEREGWISEEDLKKELGV"
     gene            3557..3877
                     /locus_tag="DHV81_07565"
     CDS             3557..3877
                     /locus_tag="DHV81_07565"
                     /inference="COORDINATES: protein motif:HMM:PF05016.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94308.1"
                     /translation="MIIKYSPVARKKLAQMKKTIGSDKVGIIVKAISDLKFNPVKCPS
                     VERMLGISSRYYFLHIEHNYIFYRIEQECIFITDIYNEKEDFMWKMFRIKLRTDESID
                     YWGE"
     gene            3904..4104
                     /locus_tag="DHV81_07570"
     CDS             3904..4104
                     /locus_tag="DHV81_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCI94309.1"
                     /translation="MKKRLYKSDKDKKVCGVCGGIANYFDVDPTVIRLAWVIFTLAGG
                     SGLIAYIIAAIIMPEDPGYIDM"
     gene            4199..4756
                     /locus_tag="DHV81_07575"
     CDS             4199..4756
                     /locus_tag="DHV81_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI94310.1"
                     /translation="MISIRTATEDDAEELLAIYAPYVKQTAITFEYDVPSKKDFKSRI
                     SHTLEKYPYLVAEEDGKIVGYAYVSPFKERAAYEWSVETSIYVDMIQKRKGIGGLLYD
                     RLEEILKKQGILNVNACIGYPQIDDEYLTKDSVYFHEKLGYHMVGTFHQCGYKFGRWY
                     DMVWMEKFIGEHTKNQAPVILYKDI"
     gene            4828..5184
                     /locus_tag="DHV81_07580"
     CDS             4828..5184
                     /locus_tag="DHV81_07580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94311.1"
                     /translation="MKINVVKRKAASNRELSHAVYDIPEVKDLRGLLYQIMLVELSAR
                     PELSSAGKIAYELSQERKYTKEQAWDILEQDFSDGLFRVFFNGEEYTNLTDVLDVQEE
                     NELVIIRLIMMAGRLW"
     gene            5178..9962
                     /locus_tag="DHV81_07585"
     CDS             5178..9962
                     /locus_tag="DHV81_07585"
                     /inference="COORDINATES: protein motif:HMM:PF13569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94312.1"
                     /translation="MVMQSGKTKEDIIKKLKEYNRSLHGYTQMEREYIKEAEDTRYLD
                     GFQIPNARIPDTLEELLKKQNNFKGLLPKGHEKLCEYLTHIQEYTCNTRLIRMDVRSS
                     LLSNYTDRIVQAIGAFYIFYGAGLSIQKLIYGEYNDVISYRNMAPVIAVGIIRQNDEV
                     IGYCRDVLTSENNVGFLTRDLIIAIEQSKNKELQDLLTNMLLAAKLQEGLRQSILETV
                     DEYQLDYFYRMIDVIYEEKLLRYSSVQRSIQTWIGLGYDEVEQRIIEQILGIIHEFIH
                     DEDKNKSALTDENPLKVYIALYCIGIKNMENAINEAVTLLLRKEQHIIAAALIYLQQT
                     QHFPFWEYKSMLENYSDNSWITALYLSECVRCEVKGMSFSKEECHELFDLVYPYLSKL
                     KAKQTYSSKGFEWFSIIIYKESIVNFLSQLLIKAPDTERIELLLPSVSILWTKALEEF
                     LKGCFSYVSLPVKKKFMIKEIISQQEALSSFVTDELKKIKLEEADILALEGRLKTKKA
                     YARAAIIEVLSKQQKESVKEIFKRLNNSQDKLIKESALELQQLVPAYFDNAPKPEVKI
                     LGREDGFGLYERFQRCDMPYQSFLQIQKKGVLIKKETVDLSFLEAWDKQKIYDYFTLW
                     NGRIEEHAKDEYQRFGMSYLVGDERLLMLDYQLDSLDAIPFGSVWREYFEKDKLSEQV
                     IFQLKFIVDGVGYDYDRLFTRDIKLTYLTSDETKKWKYYDHISKIIEYYFYECDRDNI
                     FSKPAAQTIELFLKYTKENSYRRKDSWDRNCVHSLADMDVFRALENALHLDSIEDEEF
                     KQYFPLIYDCYLHFNLDCDASVTDKMNISPVTAARACRFGILPQTVLMEMILDTHLEK
                     NGNMPYYYSGRNDMLSEAFGAAYFENRGVYGRPHLKLPEKNAQVVDYLRQTLDKISEV
                     LIHMETARLNSESSITDYVKRLYVICGMKYLLTILKVLEGEEIKRSSYGSDRQTVFAN
                     LIRRCYPLPSDSAKELEAAGIPEKRLVEVAMMAPQWIDLVNEVLDWNGFKEACYYFIA
                     HMKQDDPEHKKAEIAHYTEIDPEDLSDGAFDIDWCRDIYGKLGEKRIKTLYDASKLLC
                     ENNFHTRARKYMDACMGKKNKETFYKQASEKRNKDALNAYCIAPIEDDADLLERYLYV
                     QQFLKESKKFGSQRQASEKRSCEIALKNLARNAGFDSVDRMVWKLESSVTDEYSDVFT
                     PQQIDDIQISIAVDANGQNDICVYKNGRKLKSVPAKYKKDERVLRIKEAHQMLKQQYQ
                     RTRSMLEKAMMERTEYGLSEIESMSKHMVAGPLVRNLVMICNDSIGFYRDGYLEMGDK
                     KTKCSGTIRVAHAFDLYEHQVLKEIQQYLFDNRIVQPFKQVFRELYLKLPEELDLEYT
                     KRYTGYQIQTKQAAGALKKCGWNASYEYGLEKVSYKYDAVAQLFADADWFSPSDIEAP
                     AIDYVEFSKRRSSEPLLIRDVDDVWFSETMRDVDLAVSLAFIGGVDPVTSTSTVEVRR
                     AVIECTCRLMKLTNVRVEGNFAHIEGHCNDYSVHLGSAVVHQKAGSTIHMIPVWSGQR
                     GKVYLPFMDEDPMTAQIVSKVIMLAEDTSIKDPSILAQISKK"
     gene            10499..11374
                     /locus_tag="DHV81_07590"
     CDS             10499..11374
                     /locus_tag="DHV81_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCI94313.1"
                     /translation="MSKVIVVTDSNSGITPDEAKKLGVEVIPMPFYIDEQMYYENIDL
                     TQEQFYEKLTAGGDIKTSMPLVGDVTDKWDEFLKENDEIVYIPMSSGLSSSCETAYML
                     SQDYDGKVQVVNNQRISVTMRQSVIDAKNLAEAGKNAAEIKQILEDAKFESSIYIMVD
                     TLNYLKKGGRITPAAAALGTLLKLKPVLQIQGEKLDAFAKARTVKQAKSIMIDAMKSD
                     FEKRFNNPDGSQINLEMAYTHDIAAAEAFKEEVQAAFPNNEIVMNPLSLSVSCHIGPG
                     ALAIACSKKIEYCNK"
     gene            11472..>12745
                     /locus_tag="DHV81_07595"
     CDS             11472..>12745
                     /locus_tag="DHV81_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCI94314.1"
                     /translation="MSKVDVVIGCFYGDEGKGKVIDYLAADADVAVRCTGGDNAGHTI
                     KANGVKYAMHLIPSGLLSGHTIGVIGNGVVLNPQVLLEEINNLKEHGYDVDKYLKISE
                     KTHVIFPYHRKLDVALEKVRQAKKIGTTGKGIGPSYCDKYERSGIRMEDLYAPDFKER
                     LKEQTELKLALLKVYDPETDYTKELDFDKMFAEYSDYAAQLEPYVCDTITLLHKALED
                     DKKVVVEGAQATLLDIDFGSYPYVTSSNPTIGGILTGTGLSASDIGNVYGVIKAYSSR
                     VGEGPYVTELLDATGDKIRELGHEYGTTTGRPRRCGWLDLVTLKYAKRLNGLTALSVN
                     HLDTIGKFDTIKVCTGYDVNGEVTEDFTTNLKVLNNAKPVYEELKGGWGDEIASCKTF
                     EELPENAQKYISFIEKYIGVPVKFIGTGADREA"
BASE COUNT         4220 a         2112 c         3098 g         3174 t
ORIGIN      
        1 atggtgcttt cagaaatcac catgtcatcg tcccatattg ttccgtctat gtcaaaaaat
       61 actgttttgc tcatttgccg ctcctaatct cttcattatc atgtatggtg ctttcagaaa
      121 tcaccatgga ggctgtggcc gcccttaagg caaacggaca tcgtgcattt atatgtacag
      181 gacgtgccag agccagtgtg aggtcagaga aacttttaaa tatgggtttt gatggaataa
      241 ttgcggcatg tggcaactat attgaaatgg atggcaaagt aatatacgaa aatgacctga
      301 gcgcagatat ggtacaaaag gtcataaggg tgctttctga gtgtaaaatg cctgtagtgc
      361 ttgagggctc cacggactac tggatagatg acgagggctt tgagaatgat ccgtatgtgg
      421 attacctttt tgagtcactt ggtgagcatg cacatataat aagaggatat gacggcgaga
      481 taagaattaa taaattctcc gctgacgtga tagatggtac tgattatgaa cgtgtgaagg
      541 cagagtttgc cgatgatttt ctgatactcg agcatgtgga aaatgtagtg gagttcgtgc
      601 cgaagggaac ctcaaaggca accggcatca aatggctgtg caatcatctg gatatcccgc
      661 ttgatgaaac ctatgcgata ggcgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaat atcactgttg
      841 acattgaaat gcaatagtga tattttattt acagaaagaa attagcactc gaaagaaatg
      901 agtgctaaca gagataaaag gaggcaatac aatgaaatta gttccattat cagacagagt
      961 agtattaaag cagttagaag cagaggaaac aacaaaatcg ggaatcatcc ttacaagttc
     1021 agcacaggag aagccacagg aggctgaggt tatcgcagta ggacctggcg gaatggttga
     1081 cggcaaggaa gttaagatgc aggtaacggt cggacagaag gttatttatt ctaaatacgc
     1141 aggcacagag gttaagcttg acggagaaga gtacatcatc gttagacaga atgatattct
     1201 tgcagtagta gagtaagatt aatttacgga tagaagctaa agatccggta cgttttacat
     1261 atccggtcat agctgcttac attttggaat taaatatcaa atatataaat aaatagaaag
     1321 gtcgtataaa tcatggcaaa ggaaattaaa tatggctcag aagccagagc tgcacttgga
     1381 gctggagtag ataaattagc aaatacagtc agagtaacac ttggaccaaa aggaagaaac
     1441 gtagtacttg acaaatcata tggtgcacca ctcatcacaa acgatggtgt tacaattgca
     1501 aaagaggttg agcttgagga tgctttcgag aacatgggag cacagcttgt caaggaagtt
     1561 gccacaaaga caaatgatgt agcaggagac ggaacaacaa cagctacagt tcttacacag
     1621 gcaatggttc aggaaggaat gaagaacctt gaggcaggag caaacccaat cgtgcttcgt
     1681 cgtggtatga agagggctac agacaaggct gttgaggccc ttaaggatat gagccagaaa
     1741 gtaaagggca aagagcagat tgctaaagtt gcagctatct cagcaggaga tgaggaggtt
     1801 ggtaatctcg tagcagacgc tatggaaaag gttacaaacg atggcgttat cacaattgaa
     1861 gagtcaaaga caatgcagac agagcttgac cttgtagaag gtatgcagtt cgatagagga
     1921 tatctctcag catatatgtg cacagatatg gataagatgg aagcagtgct tgatgatcca
     1981 tatatcctta ttacagacaa gaagatttcc aacatccagg atattcttcc gttacttgag
     2041 aagattgttc aggccggtgc aagacttctt atcatcgctg aggatgttga gggcgaggct
     2101 cttacaacac ttatcgttaa caagcttcgt ggtacattca atgtagtagc tgtaaaggct
     2161 cctggctacg gtgacagaag aaaagcaatg cttgaagata tcgcagttct tacaggcggt
     2221 caggtaatca gctctgatct tggactggag ctcaaggata caacaatcga tatgcttggc
     2281 cgtgctaagt cagttaaggt tcagaaggag aacacagtca tcgttgatgg tgccggagac
     2341 aaggatgcta tcgcaggcag agtaagccag atccgtggtc agattgatga gacaacatct
     2401 gagtttgata aagaaaaatt acaggagcgt cttgccaaga tggcaggcgg tgtagcagtt
     2461 atccgtgtag gtgctgcaac agagacagag atgaaagagg ctaagcttcg tatggaggat
     2521 gcccttaacg ctacaagagc agcagttgaa gagggaatca tcgcaggtgg tggatcagca
     2581 tatatccatg catcaaagaa ggttgcagag cttgttgata cacttgaggg agatgagaag
     2641 acaggtgcaa aggttatcct taaagcactc gaggctccac tttactatat cgctgcaaat
     2701 gcaggacttg aaggtgcagt aatcatcaac aaggtaaagg agtcagctcc gggaacaggc
     2761 ttcaacgcag ctacagaaga gtatgtagat atggttgata acggaatcct tgatcctgta
     2821 aaggttacaa gaagtgcatt acagaatgca acatcagtag cttcaacact cctcacaaca
     2881 gagtcggcag tagctacaat caaagaggac actccagcaa tgccagcagg tgccggcgcc
     2941 ggaatgggca tgatgtaatc gtagacaatg aggaaaataa aaaatcagag gcaagctcgc
     3001 ttgcctctga ttttttattt gacgaatatc cggaggctgg agaatccagc cgtgtctacg
     3061 atgtaagtaa aataacgcca ctgtccggat atgagcaaga agcacgcaag tgcggattgc
     3121 gaatatacgg aggctggagt aatccagcag tgtctacgat gtaagtaaaa taacgccact
     3181 gtccagatat gagcaagaag catatctacg gctgtaaaaa tatgtgggga attaacatgc
     3241 gaatatactt gcttatattt aacgataata gtattatata gaaaaggaga tgattttatg
     3301 ccaaatatta aaccaatatc agatttaaga aactacacgg atgtattaaa agaagttgat
     3361 atatccagca gagtatattt gactagaaat ggcagaggag aatacggaat tttaactatg
     3421 gaggaaatag acgagctaga ccggttaaaa gcaatatata ctttgaataa aaagctacag
     3481 gaagcagaag atgaagctga gagagaaggc tggattagcg aagaagattt gaaaaaagaa
     3541 ttgggggtat agaaaattga taataaaata ttcacctgtg gcaaggaaga agcttgcaca
     3601 gatgaagaaa acaataggat cagataaagt aggtattata gtaaaagcta ttagtgattt
     3661 aaaatttaat ccggtgaaat gtccgtcagt tgaaagaatg ttaggaatat caagcagata
     3721 ctactttttg cacatagaac acaactatat attttataga attgagcagg aatgcatttt
     3781 tataactgat atatataatg aaaaagagga ttttatgtgg aaaatgttca gaataaaact
     3841 gagaacagat gaaagtatag attattgggg cgaataacat atatttaaaa ggagacaaca
     3901 accatgaaaa aaagattata taaaagtgat aaagacaaaa aagtttgtgg agtatgtgga
     3961 ggcattgcca actattttga cgtagatccg acagttattc gtcttgcgtg ggttatattt
     4021 accttagcag gtggaagtgg acttatcgca tacattatag cagcaattat aatgccggaa
     4081 gatccgggat acattgatat gtagattggt ctttagtaag ctgtacagcc tatgttcaaa
     4141 gcaactctat ttatatttaa tatgtaacat ggaaagtaac acaacaggag acacgcaaat
     4201 gatttcaata cgcacagcaa cagaggatga cgcagaagaa ctgcttgcca tctatgcacc
     4261 atatgtaaag cagaccgcta tcacctttga gtatgatgta ccatcgaaga aagattttaa
     4321 aagccgtatc agtcatacat tggaaaaata tccttatctg gtggcagaag aggatggaaa
     4381 gatagtaggc tatgcctatg tcagtccatt taaggagaga gcagcatatg aatggtctgt
     4441 tgagacatca atatatgtag atatgataca gaagcgaaaa ggcataggcg gactgcttta
     4501 cgacaggcta gaggaaatac ttaagaaaca gggaatatta aatgtcaatg catgtatagg
     4561 ctatcctcag atagatgatg aatacctgac aaaggacagt gtgtattttc atgagaaact
     4621 tgggtatcat atggtgggta catttcatca gtgcggatac aagtttgggc gttggtatga
     4681 tatggtgtgg atggaaaaat ttatcggaga acatacaaaa aatcaagctc ctgtgatatt
     4741 gtataaagat atataaatat atataagcaa tatataaatc caatattata taagcaataa
     4801 aggagaacag attacgaggt tacggatatg aaaatcaatg tagtaaaaag gaaagcagca
     4861 tccaacaggg aactgtcaca tgctgtctat gatatcccgg aggtaaaaga cctaagaggc
     4921 ttgctatatc agataatgct tgtggaatta tctgccaggc cggagctttc atcagcggga
     4981 aagatagcat atgagctgtc acaggagaga aagtatacaa aggaacaggc atgggatatt
     5041 ctggaacagg atttttcaga tggactgttc agggtatttt tcaatggaga ggaatataca
     5101 aatcttacgg atgtattaga tgtgcaggaa gaaaatgagc ttgtgataat caggcttatc
     5161 atgatggcgg ggaggttatg gtaatgcagt cagggaaaac aaaagaagat ataataaaaa
     5221 aactaaaaga atacaaccgt tcgctccatg gttatacgca gatggagagg gaatatatta
     5281 aagaagcgga ggataccaga tatctggacg gctttcagat accaaacgca aggataccgg
     5341 acacactgga ggaactttta aaaaagcaga ataattttaa aggattgctt ccaaaaggtc
     5401 atgaaaaact gtgcgaatat ctgactcaca tacaggagta tacgtgcaat acacggctta
     5461 tccgcatgga tgtaaggagc agtctgctgt caaattacac agatcgtatc gtacaggcaa
     5521 taggcgcatt ttacatattt tacggtgcag ggctatcaat acagaaactc atatacggag
     5581 agtataatga cgttataagc tatcgaaata tggcacctgt gatagctgtt gggataataa
     5641 ggcaaaatga cgaggttatc gggtattgcc gggatgtgct gaccagtgaa aataacgtag
     5701 gttttctgac tcgtgacctt ataatagcaa tagagcagag caaaaataaa gaattgcagg
     5761 atctgcttac caatatgctt ttggcggcaa aactgcagga gggattgagg cagagcatac
     5821 ttgagacggt ggatgagtat cagcttgact atttttaccg catgatagat gtgatatatg
     5881 aggaaaaact gctcaggtat tcgtccgtac agcggagcat tcagacatgg atcggcttag
     5941 gctatgatga agtcgagcag aggatcatag agcagatact tggaataatt catgagttta
     6001 tacatgacga ggataaaaac aaaagcgcgc ttacagatga aaatccgtta aaggtatata
     6061 tagcactgta ctgtatcggt ataaaaaata tggaaaatgc cataaatgag gcggtaactt
     6121 tacttctgag aaaagagcag catatcatag cggctgcgct tatatatctg cagcagacac
     6181 agcattttcc gttctgggaa tacaagtcaa tgcttgaaaa ttacagtgat aatagctgga
     6241 taactgcgtt gtatcttagc gaatgtgtca gatgtgaagt aaaaggcatg tcattttcaa
     6301 aggaagaatg tcatgagctg tttgatctgg tatatccgta tctgtcaaag cttaaggcaa
     6361 agcagacata ctcatcaaag ggcttcgagt ggttttcaat cattatatat aaggaaagta
     6421 ttgtaaactt tctgtcacaa ctgcttatca aagcaccgga tacagagagg atagaactgc
     6481 tgctgccaag tgtatctata ttgtggacaa aggctttgga agagttctta aagggctgct
     6541 tttcgtatgt atcacttcct gtaaagaaaa aattcatgat aaaagagata atatcacagc
     6601 aggaggctct tagcagtttt gtaacggatg aattaaaaaa gatcaagctt gaggaggctg
     6661 acattcttgc acttgaggga cgtcttaaga ccaaaaaggc atacgcaaga gcagcgatca
     6721 tagaggtgct ttcaaaacag caaaaagaaa gcgtaaagga gatttttaaa cgtttaaata
     6781 actcacagga caagctgata aaggagtctg cacttgagct gcagcagctg gtgccggcat
     6841 attttgacaa tgcaccaaag ccggaggtaa agatacttgg cagggaagat ggattcggac
     6901 tgtacgagcg ttttcagaga tgtgatatgc cgtatcagtc atttttgcag atacagaaaa
     6961 aaggtgtgct gataaagaaa gagactgttg atctaagttt tctcgaggcg tgggacaagc
     7021 agaagattta tgactatttt actctgtgga atggacgtat cgaggaacat gcaaaggatg
     7081 aatatcagag atttgggatg tcgtatctgg tcggtgacga gcgtcttctg atgctcgatt
     7141 atcagctgga ctcattggat gcgattccgt ttggcagtgt atggagagaa tattttgaaa
     7201 aagataagct gagtgagcag gtgatctttc agcttaaatt tattgtggac ggtgttggat
     7261 atgattacga taggctgttt acaagggata taaagcttac atacctgact tctgatgaga
     7321 cgaaaaaatg gaaatattat gaccatatct caaagatcat tgaatactat ttttacgaat
     7381 gtgacaggga taatatattt tcaaaaccgg cagcacagac tattgagctg ttccttaagt
     7441 atacaaaaga aaattcttat cgcaggaagg attcctggga caggaactgt gttcattcgc
     7501 ttgccgatat ggatgtattt cgcgcgttgg agaatgcgct tcatctggac agcatagagg
     7561 atgaagaatt taagcagtat tttccgctga tctatgactg ctatctgcat tttaacctgg
     7621 actgtgatgc atctgtgacg gataagatga atataagccc tgtgacagca gcacgcgcat
     7681 gccgatttgg aatactgccg cagaccgtgc tgatggagat gatactggat acacatctgg
     7741 aaaaaaatgg caatatgcca tattattaca gtggcagaaa tgatatgctt tcagaggcat
     7801 ttggcgcagc atattttgaa aacagaggtg tatacggcag accacatctg aaactgccgg
     7861 agaaaaatgc gcaggtagtt gattatctaa gacagactct tgacaaaata agcgaggttc
     7921 tgatacatat ggagacagcg cgccttaaca gcgaaagcag cataacggat tatgtaaagc
     7981 ggctttatgt tatctgcggc atgaaatatc tgcttaccat attaaaagtg cttgagggag
     8041 aggagataaa gcggtcatcg tatggtagtg accgtcagac tgtctttgca aatcttatcc
     8101 gcagatgtta tccgcttccg tcagactctg caaaagagct tgaagctgcc ggaatacctg
     8161 aaaaacgtct cgtagaggtg gcaatgatgg cacctcagtg gatagacctg gtaaatgagg
     8221 tgcttgactg gaatggtttc aaggaggcat gctactactt tatcgcacat atgaagcagg
     8281 atgatccgga acacaaaaag gcagagatag cacactatac ggagattgat ccggaagatt
     8341 taagtgatgg agctttcgat atagactggt gcagggacat ttacggtaag cttggcgaaa
     8401 agcgcataaa gacgctctat gatgcatcaa agctgttgtg cgaaaacaac ttccacacga
     8461 gggcaagaaa atacatggat gcatgtatgg gtaaaaagaa taaagaaact ttttacaaac
     8521 aggcatcgga gaagcgtaat aaggatgcgc tgaatgcgta ctgtatcgca ccgatagagg
     8581 atgatgcaga cctgctcgag agatatcttt atgtacagca gtttttaaaa gagtcaaaaa
     8641 aattcggctc tcagagacag gcgagtgaaa agcgaagctg cgagatagca cttaaaaatc
     8701 ttgcaagaaa tgcaggattt gacagtgtgg atcgtatggt atggaagctt gagagcagtg
     8761 tgacggatga gtatagtgat gtgtttactc cacaacagat tgatgatata cagatatcga
     8821 tcgcagtgga tgcaaacgga caaaacgaca tctgtgtata caaaaatggc agaaaactca
     8881 aatccgttcc cgcaaaatac aaaaaggacg agcgtgtact ccgcataaaa gaagcccatc
     8941 agatgttaaa gcagcagtac cagcgcacac gcagcatgct tgaaaaggca atgatggagc
     9001 gcacggagta tggtctttcg gaaatagagt caatgtcaaa acatatggtt gcaggtccac
     9061 tcgtaagaaa tctcgttatg atctgtaatg acagcatagg tttttacagg gacggatatc
     9121 tggaaatggg agataaaaag acaaaatgca gtggcacgat tcgtgtggca catgcatttg
     9181 atctctatga acatcaggta ttaaaagaga tacagcagta tttgtttgat aacaggatcg
     9241 tacagccgtt taaacaggtg ttcagagagc tgtacttgaa attgcctgag gagctggatc
     9301 ttgagtacac taaacgctat acaggatatc agatacagac aaagcaggct gccggagcat
     9361 taaagaaatg cggctggaat gcatcatatg agtatgggct ggagaaggta tcttataaat
     9421 atgatgcggt agcacaactg tttgcagacg cggactggtt ttctccaagt gatatagagg
     9481 cacctgccat agattatgtg gagttttcaa agagaaggag cagtgagccg cttctgatcc
     9541 gcgatgtgga tgatgtatgg ttttcggaga ccatgaggga cgttgatctg gcggtaagtc
     9601 tggcatttat cggaggcgta gatcctgtta cttctacatc tacagtagag gttagaagag
     9661 cagtgatcga gtgtacctgc aggctcatga agcttacaaa tgtcagggtg gaaggcaatt
     9721 ttgcacatat agagggacat tgtaatgatt acagcgtcca tctgggaagt gccgttgtgc
     9781 atcagaaagc aggcagcacg atacatatga tcccggtatg gagcggacag aggggaaaag
     9841 tatatctgcc atttatggat gaagatccga tgactgcgca gatagtatca aaagtgatca
     9901 tgctggcaga ggatacatcg ataaaggatc catccatact ggcacagatc agtaaaaaat
     9961 aaaaaagagg cgataacaaa tgaacaaaaa gatggtaaac cgtatcatca tctatgtggc
    10021 aggattactc gttcttgccc ttggaataat attaaacaca gaggcggggc ttggagtctc
    10081 accgattatc tcggtatcgt acagcatatc gcagatcaat ggctttaact ttggaaacac
    10141 gaaaatcgtt tgatagattt agaaatctgc ttatcatttt gagtcgcttg tgttatactt
    10201 ttattcatag catgagcctc cggtttgtaa ttgtttttcg acaactcaat tataccaaaa
    10261 ccattggttt catgctattt ttatgccagt tattattgaa ttttcaaggt gcgtaggcac
    10321 attttaatgt gcgaagtttg agtaaataat agaataaaca gtgtatacgt tttagcgtcc
    10381 tgatattgta aatattatca gggcgctaaa aacttctttt caaacaatta cacctatggt
    10441 ataatgagcg gtgttggaaa aaatacgaca acgaatattt atatcggagt gagaattaat
    10501 gagtaaggtt atagtagtta ccgattcaaa cagcggtatc acacctgacg aggcaaagaa
    10561 gcttggagtt gaggtgattc caatgccttt ttacatcgat gagcagatgt attatgagaa
    10621 tatcgatctg acgcaggagc agttctatga gaagcttacg gcaggcggcg atatcaagac
    10681 atctatgcct ttggttggtg atgtaacaga taaatgggat gagttcttaa aggaaaatga
    10741 tgagattgta tacattccaa tgtcatcagg tctttcaagc tcatgtgaga cagcatatat
    10801 gctctcgcag gattatgacg gcaaagtgca ggtagtcaat aatcagagaa tatcagttac
    10861 aatgcgccag tcggttatag atgcgaagaa cctggcagaa gcaggaaaaa atgcagctga
    10921 gataaagcag atattagagg atgcaaagtt tgagtcttcc atatatatca tggtagatac
    10981 tcttaactac ctcaaaaagg gtggtagaat cacgcctgca gcggcagctc ttggcacact
    11041 tttaaagctt aaaccggtgc ttcagattca gggtgagaag cttgatgcat ttgcaaaggc
    11101 acgtaccgta aagcaggcaa agagtatcat gatagatgcc atgaagtcgg attttgagaa
    11161 gcgcttcaat aatccggatg gcagccagat aaatcttgaa atggcataca cgcatgatat
    11221 tgcagcagcg gaggcattta aggaagaggt tcaggcagca tttccaaata acgagatagt
    11281 catgaatccg ctttcacttt cggtctcatg ccatatagga cccggagcgc ttgcaatagc
    11341 ctgctctaag aagatagagt attgtaataa ataattatta atagttacaa agcataataa
    11401 atataccact aaaatcaagc agtaacatgg cacaatgtgt cgtgtcagaa aatacaaggg
    11461 aggcatgtaa aatgtcaaag gtagatgtag taataggatg tttctatggt gatgaaggaa
    11521 aaggtaaggt aattgattac cttgcagcag acgctgatgt agcagtaaga tgcacaggcg
    11581 gagacaatgc cggtcacaca atcaaagcaa acggtgttaa atatgcaatg caccttattc
    11641 cgtcaggact cttatcagga catacaatcg gagtgatcgg aaacggagta gttttaaatc
    11701 cgcaggttct tttagaggaa atcaacaacc tgaaggagca cggatatgat gtagataaat
    11761 acttaaaaat cagtgaaaaa acgcatgtca ttttcccata tcacagaaag cttgatgtgg
    11821 cgcttgagaa ggtgagacag gcaaagaaaa tcggtactac aggaaagggt atcggacctt
    11881 catactgcga taagtatgag agaagcggaa tcagaatgga ggatttatac gcacctgatt
    11941 tcaaggagcg ccttaaggag cagacagagc ttaagcttgc acttcttaag gtatatgatc
    12001 ctgagactga ttatacaaag gagcttgatt tcgacaagat gtttgcagag tacagtgact
    12061 atgcagcaca gcttgagcca tatgtatgtg atacaatcac cctcttacac aaggcacttg
    12121 aggatgacaa aaaggtggtt gttgagggag cccaggctac actccttgac atcgactttg
    12181 gttcatatcc atatgttaca tcatcaaatc ctacaatcgg tggcatcctt acaggaacag
    12241 gtctttctgc ttcagatatc ggaaatgtat acggtgtcat caaggcatac tcaagccgtg
    12301 taggtgaggg accatatgtg acagagcttc ttgacgcaac cggagacaaa atccgtgagc
    12361 tcggacatga gtacggtacc acaacaggcc gcccgagaag atgcggatgg ttagaccttg
    12421 tcaccttaaa atatgccaag cgtcttaacg gacttacagc actttctgta aaccatctcg
    12481 acacaatcgg caagtttgat acaataaaag tctgcaccgg ctacgatgta aacggtgagg
    12541 taacagagga ctttaccaca aatctcaagg ttttaaacaa cgcaaagcct gtatatgagg
    12601 agctaaaagg cggctggggc gatgaaatcg caagctgtaa gactttcgag gagcttcctg
    12661 agaatgcaca gaaatacata agcttcatag aaaaatatat cggagtacct gttaagttca
    12721 ttggaacagg tgctgacaga gaagc
//
LOCUS       DPUF01000214            5344 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23104, whole
            genome shotgun sequence.
ACCESSION   DPUF01000214 DPUF01000000
VERSION     DPUF01000214.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23104"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="DHV81_07600"
     CDS             complement(<1..1278)
                     /locus_tag="DHV81_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Lon protease"
                     /protein_id="HCI94315.1"
                     /translation="MPVFDFSDTPVANEHPECTYETFHSTETKASADKPIVVIDNKQK
                     EWAHHALGMFTNPIKRTSFEFEDGDGVTSADILRIDSRFVSLFKWLGENHIKVQLSGA
                     NTPDGYAVYRVREVALGRGTKLSAEDGFLQYMMERLFASTMPEDEPDLDDYEEEGDSM
                     KLTSIQSITDFMTCAGRTLPDNILLWARRNLAVARSSEVSPEERRHAQRALSIMMNIQ
                     WKGNYFEAIDPVEARRILDEELYGMESVKQRIIETIIQINRTHTLPAYGLLLCGPAGT
                     GKSQIAYAVARILKLPWTTLDMSSINDAEQLTGSSRIYSNAKPGIIMEAFSQAGESNL
                     VFIINELDKASSKNGNGNPADVLLTLLDNLGFTDNYMECMVPTVGVYPIATANDKSQI
                     SAPLMSRFAVIDIPDYTDEEKKVIFQRFALRKVF"
     gene            complement(1325..2086)
                     /locus_tag="DHV81_07605"
     CDS             complement(1325..2086)
                     /locus_tag="DHV81_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCI94316.1"
                     /translation="MLNQFSRTRLLLGKEAMDRLKGARVAVFGIGGVGGYVCEALVRS
                     GVGAIDLIDDDKVCLTNLNRQIIATRKTIGKYKTDVMEERIHDINPDVSVVTHRCFFL
                     PENADEFPFEEYDYIVDAVDTVTAKIALVMKAQEKNVPIISSMGAGNKLDASAFKVAD
                     IYKTSMCPLAKVMRRELKKRGVKKLKVVYSEEKPTRPIEDMSISCRTNCICPPGATHK
                     CTERRDIPGSLAFVPSVAGLIIAGEVVQDIAFDRV"
     gene            complement(2087..3265)
                     /locus_tag="DHV81_07610"
     CDS             complement(2087..3265)
                     /locus_tag="DHV81_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94317.1"
                     /translation="MADIMDYIDWRGDIGFDEVHVNEVDGLIFSQLIYVQMKPYMPDA
                     KKSYLTIKQLSSLYCADHSDDEIEQMPNLFRHSARLLQKLAHSRRYADCILRYYIYDI
                     SEKEESQFSAVTIELPDGTYFISYSGTDHSVVGWKENFNLSYLDETPGQNKAKKYLKQ
                     VAAYICNDDRGINEKAINDKALDDENINNKSINTGKLWIGGHSKGGNLAVFAAMHVDK
                     EVQDVIIKVFNFDGPGFNHKMIYTAGYKRIFDRIETFLPQSSIVGLLLEHVDDYEVVR
                     SRNSGPLQHDAFSWEIMGGSIIKADGLDKNSVRLDKTLRNWIGSMDEAQRKQFVNVLF
                     SIASDSNFENLDQMSFKQLIEMIKAADELSKADWSMLKDTVRLLISAGVGVVRDEKEK
                     "
     gene            complement(3293..4426)
                     /locus_tag="DHV81_07615"
     CDS             complement(3293..4426)
                     /locus_tag="DHV81_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCI94318.1"
                     /translation="MQLSIEEVKKLDSNSYQIIDIRSEEEVAHGAIKGALNIQADEIE
                     SDERVLHDKKLVIVCSRGKTSVDVAEYLTEKGFDAASLKGGYISWLLDAMKEDEVAER
                     DIKADVEQSIRKKFKKSIWRKFTKAINTYELVKPGDKIAVCISGGKDSMLMAKLFQEL
                     KHHNKFDFDVKFLVMDPGYNEANRHVIEENCRNLGIPATIFESDIFDAVYDIEKSPCY
                     LCARMRRGHLYAFAKELGCNKIALGHHYDDVIETILMGMLYGAQVQTMMPKLHSTNFE
                     GMELIRPLYLVREDDIKAWRDYNGLHFIQCACKFTDTCTTCNNEENRSKRVEIKQLIA
                     NLKKVNPFVEANIFKSVENVNLATVVAYKKDGIKHSFLDHYDE"
     gene            complement(4442..4900)
                     /locus_tag="DHV81_07620"
                     /pseudo
     CDS             complement(4442..4900)
                     /locus_tag="DHV81_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742220.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    4604..4703
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5083..5344
                     /locus_tag="DHV81_07625"
                     /pseudo
     CDS             5083..5344
                     /locus_tag="DHV81_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742219.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
     assembly_gap    5154..5180
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1329 a         1368 c          941 g         1579 t
ORIGIN      
        1 aaataccttg cgcagggcaa atctctggaa gatgaccttc ttctcttcat cggtatagtc
       61 cggaatatca atcaccgcaa aacgtgacat aagcggtgca gaaatctggg atttatcgtt
      121 ggctgtggca atcggatata ctcctactgt cggcaccata cactccatat agttgtctgt
      181 aaagcccaga ttgtcaagca gtgtcaaaag cacatctgcc ggatttccgt taccgttttt
      241 gcttgaggcc ttgtcaagct cattgatgat aaatacaaga tttgactctc ccgcctgaga
      301 aaatgcctcc ataatgattc ccggctttgc atttgaatag attcttgagc ttcctgtgag
      361 ttgctccgca tcatttatgg agctcatgtc gagtgttgtc catggaagct taagtatacg
      421 cgcaaccgca tatgcaatct gtgactttcc cgttcctgcc ggtccgcaga gcagcagtcc
      481 gtaggcagga agtgtatgcg tcctgtttat ctggataata gtctcaataa ttctctgctt
      541 cacactctcc atgccataga gctcctcatc gagtatgcgt cttgcctcca cagggtcaat
      601 tgcctcaaag taatttccct tccactggat attcatcatg atggaaagtg ctctctgcgc
      661 atggcgtctc tcctcaggtg atacctcgct tgaacgcgca actgccaggt ttcttctggc
      721 ccacagcagt atattgtcag gcagtgtacg gcccgcacag gtcatgaaat ctgtgatgct
      781 ttgtatactg gtgagcttca tgctgtcgcc ctcttcctcg taatcatcga ggtccggctc
      841 atcctcaggc atggtgctcg caaagagcct ctccatcata tactgcagga agccgtcctc
      901 cgcagacagc tttgtgcctc tgccgagagc cacctctctc accctgtata ccgcgtatcc
      961 gtcaggtgta ttggctcctg aaagctgaac ctttatgtga ttttcgccaa gccacttaaa
     1021 aaggctcacg aagcgcgaat caatgcgaag gatatcggca ctggtcacac catcaccatc
     1081 ctcaaactca aaggatgtac gctttatcgg atttgtaaac ataccgagcg catgatgcgc
     1141 ccattccttt tgcttattgt ctatcacaac aatcggttta tcggccgatg cctttgtctc
     1201 tgtcgaatga aatgtctcat atgtacactc cggatgctcg tttgctaccg gtgtatcgct
     1261 aaaatcaaat actggcattt ttattcctct ctttttgtaa ttatattatt aattattttc
     1321 ttcatcatac cctgtcaaag gctatgtcct gtaccacctc gcctgcgatg ataagccccg
     1381 ctacggatgg cacaaatgca agacttcccg gaatatccct gcgctcagtg cacttgtgag
     1441 tggcacccgg cgggcagatg cagttggttc tgcagcttat ggacatatcc tcaatcggtc
     1501 ttgtaggctt ctcctcagag tataccacct taagcttttt cacgccacgt tttttaagct
     1561 cacggcgcat gacctttgca agaggacaca tgctggtctt atatatatca gccaccttaa
     1621 atgcacttgc atccagctta tttcctgctc ccatgctgct tatgataggg acatttttct
     1681 cctgtgcctt catgacaagc gctatctttg ctgttaccgt atcgaccgca tcaactatat
     1741 aatcatactc ttcaaacgga aattcatcag cattttccgg caaaaagaag cacctgtgcg
     1801 tcacgacact gacatcagga ttaatatcat gaatcctctc ctccatcaca tctgtcttgt
     1861 attttcctat ggtctttcgg gttgcgatta tctgcctgtt tagatttgta agacacacct
     1921 tgtcatcatc gataaggtca atcgctccca cgccgcttct gactagtgcc tcgcagacat
     1981 atccgcccac tcctcctatt ccaaatactg cgactcttgc gccctttagc ctatccatcg
     2041 cctcttttcc aagcaaaagc cgtgttcttg aaaactgatt taacatttat ttctcctttt
     2101 catccctgac cacgccgaca ccggcagaaa tcaggagtct tactgtatcc ttaagcatac
     2161 tccagtcagc ctttgacagc tcatcggcag cctttatcat ctcaatgagc tgcttaaatg
     2221 acatctggtc aaggttctca aaattggagt ccgatgctat tgaaaaaagc acatttacaa
     2281 actgctttcg ctgcgcctca tccatgctgc ctatccagtt tctgagtgtc ttgtcaaggc
     2341 gcacactgtt tttgtccagc ccatccgcct ttatgatact tccgcccatt atctcccatg
     2401 aaaaagcatc atgctgcagc ggccccgaat tacggcttct taccacctca taatcgtcca
     2461 catgctcaag caataagcca acaatagacg actgtggcag aaatgtctct attctgtcaa
     2521 aaattctttt gtagcctgcc gtatatatca tcttgtgatt gaagcccggc ccgtcaaaat
     2581 taaacacctt aataatgaca tcctgcacct ctttatccac atgcatggcg gcaaacactg
     2641 ccagatttcc accctttgag tgtccgccta tccacagctt tcctgtattg atgcttttat
     2701 tgttgatatt ttcatcatct aaagctttat catttatggc tttttcattt atgcccctat
     2761 catcattgca tatatatgct gccacctgct ttaaatattt tttcgccttg ttctgtcccg
     2821 gtgtctcatc gagatagctt agattaaagt tttccttcca gccgaccacg gaatggtctg
     2881 taccactgta ggaaatgaaa tatgtgccgt cgggcagttc tatcgtaacc gcagaaaact
     2941 gcgactcctc tttttcacta atatcgtata tataatatct caaaatgcag tcagcatagc
     3001 gccttgagtg cgccagcttt tgaagcagcc tcgcggagtg gcggaaaaga ttaggcatct
     3061 gctcaatctc atcatcactg tggtccgcgc agtaaaggct tgaaagctgt tttatcgtaa
     3121 ggtatgactt ctttgcatcc ggcatatatg gcttcatctg cacataaata agctgtgaaa
     3181 aaatcagtcc gtccacctca tttacatgca cctcatcaaa gcctatgtct ccacgccagt
     3241 caatataatc catgatatct gccatacaat gccctcctga aatatatctg tcttattcat
     3301 catagtgatc taggaagctg tgctttatgc catccttttt atatgctaca accgtagcca
     3361 gatttacatt ctccacgctc ttaaatatat ttgcctcgac aaaaggattc acctttttaa
     3421 gattagcgat gagctgcttt atctcaacac gctttgagcg gttttcctca ttattgcagg
     3481 tggtacaggt atctgtaaac ttgcaggcac actgtatgaa atgcagaccg ttgtaatcac
     3541 gccatgcctt tatatcgtcc tccctgacca ggtaaagagg cctgataagc tccatgccct
     3601 caaagtttgt gctgtgaagc ttaggcatca tggtctgtac ctgtgcaccg tagagcatgc
     3661 ccatgagtat cgtctcgata acatcatcat aatgatgtcc gagcgcaatt ttgttgcagc
     3721 ccagctcctt tgcaaatgca tacagatgtc ctcttctcat cctcgcacat aaatagcatg
     3781 gcgatttttc tatatcatat acagcatcaa atatatctga ctcaaaaata gtggcaggga
     3841 tgccaaggtt cctgcagttt tcctctatga catgcctgtt tgcctcattg tagcccggat
     3901 ccatgacgag aaacttcaca tcaaaatcaa acttgttgtg atgcttaagc tcctgaaaaa
     3961 gctttgccat gagcatggaa tccttacctc ccgaaataca caccgcaatc ttatcccccg
     4021 gctttacaag ctcataggta tttatcgcct ttgtaaactt gcgccagatg gatttcttaa
     4081 atttctttct gatgctttgc tccacatctg cctttatatc gcgctctgcc acctcatcct
     4141 ctttcatggc atcaagcagc cagcttatat agcctccctt taagcttgcc gcatcaaaac
     4201 ctttttctgt gagatactct gccacatcca cactcgtctt accacggctg cagactatga
     4261 caagcttctt atcatgcaac accctctcat ctgactctat ttcatctgcc tgaatattga
     4321 gtgctccctt tatggcaccg tgcgccacct cttcctcact tcttatatct attatctgat
     4381 agctgttact atccagcttt ttaacttctt ctatactaag ctgcatttta tttctcctat
     4441 attatttccg caatgacttt atttcctgaa atatcctgta ctcataatat gccgtcacca
     4501 caaggatgag ccctatcata atgaggctgt atacatcgcg gattgctatt ccaaacgcat
     4561 cacacacgca tggcactaaa agccccgcag taaaaacaag cttnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnagctttt ccggataatg ctgcagccat gtgattgcaa
     4741 tgtatacaag caaaagcaca agtatattgg ccacaaactt aaatacctgt atgtgatagc
     4801 acactccata ataaatctgt aagcctatat acgcaagtgc taaaatcgca tctatcgcct
     4861 caagcaccgc cgaagcaaag gtcatatctc tttttctcat atcaatctac tgcctttctt
     4921 aacaccctga ccacgtaaaa ataaggtaaa actatattgt ttcgctttaa actatctaat
     4981 tttatcatat tatggactat ctttctagaa aaattgtgat ataataatca ggttaaagga
     5041 attttattta aaaattaata atacaacgag gtttaatcga aaatgagcaa attaacaata
     5101 ccaaagaact attcttcggc actgaatctt cacgatacac agcttgccat caannnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn actgttaagg atttcttcca gggcatgctc gcacagcgtc
     5221 tttccctgac acgtgtttca gcaccgcttt tcgttgatcc taccacaggc ttaaacgata
     5281 acttaaacgg atatgaaaga cctgttacct tcggcatcct tgagcagggt gacaaggagg
     5341 ctga
//
LOCUS       DPUF01000215            8190 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13937, whole
            genome shotgun sequence.
ACCESSION   DPUF01000215 DPUF01000000
VERSION     DPUF01000215.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13937"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            1..1634
                     /locus_tag="DHV81_07630"
                     /pseudo
     CDS             1..1634
                     /locus_tag="DHV81_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743160.1"
                     /note="too many ambiguous residues; incomplete; missing
                     start; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     assembly_gap    380..458
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1969..2940
                     /locus_tag="DHV81_07635"
     CDS             1969..2940
                     /locus_tag="DHV81_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCI94319.1"
                     /translation="MSNKTNAVVKTNPRKAVMIGCGFVGSASVFALMQSGLFSEIVLI
                     DADKDKAEGEAMDISHGIPFARPMKIYAGGYDDVADAAIIVVSAGAGQKPGETRLDLV
                     NKNVAIFKSIIPEIAKRNFGGILLIVANPVDILTQVAQKLSGLPQERVIGSGTVLDSA
                     RLKYALGEHLGVDSRSVHAFIVGEHGDSEVVAWSSANVSGVELHKMCEMRGHYKHKEN
                     TEEIAANVKNSAYEIINRKHATYYGIAMSVRRICEVIMRDEKSILPVSHMMHGQYGID
                     GVVLSMPAIVGANGVESDIPIELNGEEALKLKESADALKSILDGLEL"
     gene            3117..3974
                     /locus_tag="DHV81_07640"
     CDS             3117..3974
                     /locus_tag="DHV81_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3737 domain-containing protein"
                     /protein_id="HCI94320.1"
                     /translation="MSERKQIHQEFLTGERALFASNDLDIYDTIFDDGESPLKESHGI
                     SLTGCFFRWKYPLWYCSDIAVKDCTWFDMARAGVWYTDNISVTDSIVEAPKNFRRCNN
                     LKLKNVTFPNAQETLWSCDHVXXXIENVSAKGDYFGMNTNNLIMKNFQLVGNYSFDGS
                     RNVEVHDSKLISKDAFWNTENVTVYDSFITGEYLGWNAKNLTLINCTIESLQGLCYID
                     NLVMKNCKLINTTLAFEYSSVDAEICSKVDSVLNPTSGVIRAKEIGILTIEKDRVDPS
                     KTKIICEGK"
     gene            3978..5126
                     /locus_tag="DHV81_07645"
     CDS             3978..5126
                     /locus_tag="DHV81_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCI94321.1"
                     /translation="MYDFDTVVDRRNTGSLKWDVAENELPMWVADMDFKTAPQIIDAI
                     SERVSHGVFGYSIIPDEWNDAYISWWDRRHGLKIERDSLIFCTGVVPAISSTVRKLTT
                     PGENVLIMTPVYNIFFNSIFNNGVNVQESPLGYENGRYFVDYDRLEHDISNPQTTLMI
                     FCNPHNPCGIIWTKDELAKIGALCRKYNVTVISDEIHCDITAPGKSYVPFAAASEDCA
                     DISITCIAPTKCFNMAGLQTAAVMVPNKFLRHKVWRALNTDEVAEPNAFAITAAVAAF
                     TKGEPWIEELRKYLWENRKTVCDFVAENLPQIHVVDADATYLMWLDCSALTDDSVAFT
                     QMIREKTGLYLSEGAEFGGNGRYFVRLNIACPGSVLMEGLQRLKRALV"
     gene            5433..5798
                     /locus_tag="DHV81_07650"
     CDS             5433..5798
                     /locus_tag="DHV81_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCI94322.1"
                     /translation="MRNNESSENYLETILILSKKLPVVRSVDIANELGFKKSSVSIAM
                     KNLREKNNITVSDAGFITLTESGRQIAEMIYERHELLSECLEKLGVDKEIAAEDACRI
                     EHVISPESFEAIKKHIHKS"
     gene            5946..7319
                     /locus_tag="DHV81_07655"
                     /pseudo
     CDS             5946..7319
                     /locus_tag="DHV81_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568121.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     assembly_gap    6524..6570
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7320..7510
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7511..7714
                     /locus_tag="DHV81_07660"
     CDS             <7511..7714
                     /locus_tag="DHV81_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCI94323.1"
                     /translation="MHGDKTIFIVSQRTSSIQFADNIIVLDDGQMVGFGPHEKLLETC
                     ETYKEIYDSQFKKESDMTKQKEV"
     gene            7718..>8190
                     /locus_tag="DHV81_07665"
     CDS             7718..>8190
                     /locus_tag="DHV81_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCI94324.1"
                     /translation="MAMDNAKMNKAGTMRKVLSYMKRYIPLLVISLALSVVTVALTLY
                     FPILTGRAIDLIVGKEKVDFTAMTAILTRAAIIVVIAAAAQWLTNICNNRMTYNIVKD
                     IRHDAFLNIEKMPLSYIDSHSHGDMVSRIIADVDTFSEGLLMGFTQLFTGIATIAG"
BASE COUNT         2435 a         1468 c         1913 g         2051 t
ORIGIN      
        1 ttgtacatct gtacaattcc acatctgttg cacagcgtga gcaggtgttt aagaagtcaa
       61 aggaagagat aaagcagctt gcagttgacg gagctatttt actgagagac cttgcagcag
      121 agactgacgg taatttccgt ttccagtatt caccggagag cttttcgggc actgaggtgg
      181 attatgcggt tgatgtgtgc aacgctgtgc ttgatgtatg gcagccaaag gctgataata
      241 aggccatcat caatatccct accacagtag agaatgctat gccacatgtt tttgccacac
      301 agatagagta tatcaacaag cacctcaagt acagagacgg agtagtgctt tgcttacatc
      361 ctcataatga cagaggctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg tgagagaaca ggaaatgtag
      481 atatcgtgac acttgccatg aatctttact cacacggagt ggatccacag cttgattttt
      541 caaatctgcc tgagatcagg gagaagtatg agacatttac cagaatgaag gtaggcgaga
      601 gacagccata tgcgggagac cttgtttttt ctgctttttc aggctcacat caggatgcga
      661 tctcaaaggg catggcatgg agagaggaaa agaagcttga taagtggacg gttccatatc
      721 tgcctataga tccaaaggat gtcggcagga catacgaggc tgatgttatc cgtatcaaca
      781 gccagtcagg aaagggcgga gtggcatata tccttaagca gagcttcggt atcaatgtgc
      841 cacagcagat gagagagcag gtcggataca tggtcaagca ggtatcggac gaggagcaca
      901 aggagctttc acctgagtgg gtacacagta tctttacaga caattatgtg gattttcatc
      961 catatttcac tataccggag tgccacttta agcaggtaaa cggcattttc gcagaggcag
     1021 ttattttaca caatgacagc accagaaagg tagatgccaa tggaaacggc cgtctcgatg
     1081 cagtcagcaa tataattaag cagtattttg acatcagctt tgagctcact gtatacgagg
     1141 agcatgcgct ttcacacgga tcttcatcaa aggctatggc atatgtgggt atcacagttg
     1201 acggcagtat gttctggggc gcaggtgtgg atgaggatat catcaaggca tcaatcggag
     1261 cgcttgttgt tgcggtaaac cattaccttg ccaccaaggc tgacagtcac gtgaaggatg
     1321 agcgctatat tgccatgctc aacttcatcc aggccaacta caagacagta tcgcttgcag
     1381 acatggctgc acagttcaac ctgacagagc cgtatatctc caagtacata aaggagaagt
     1441 caggcaagac cttcggagag ctggtcacca atgccaagat gaagaaggca cgcacactgc
     1501 ttagaaacag catcacaact gttgagaata tcgcaattga ggcaggatat cagaacgtgg
     1561 agcattttaa cagacagttc aagaagctgt atggaatgac accgcttgag tacagaaatt
     1621 caagcacaaa ataagcggat acagcattta aatataaccg gatcaatatg ctaaatgaca
     1681 attcattcag ctgtattggt ccggtttgct tttagtattg cactttttat gtgaaaatct
     1741 gcattaaagt tgtataaaaa tgggaacaat gggctattga ttgtatttta tcaagtaatt
     1801 tgttgttttt acaacggatt atatgtacaa tgcgggtata atataaataa gagataatta
     1861 ttcagaacaa ctgataacaa agcagatatt gagaatttgt gtttgggatg aatagttata
     1921 ataaaaagca actatttgaa ttatagttta agaaagagag gaacgtacat gagcaataaa
     1981 actaacgcag ttgttaagac aaacccaaga aaggctgtca tgatcggatg tggattcgtt
     2041 ggttcggcat cagtttttgc acttatgcag agcggtcttt tttctgagat agttctcatt
     2101 gacgctgata aggacaaagc tgagggagag gcgatggata taagccacgg tattcctttt
     2161 gccagaccta tgaagatata tgcaggtggt tatgatgatg tggcagatgc tgctatcatt
     2221 gttgtttctg caggtgcagg ccaaaaaccg ggcgagacaa gacttgatct ggtaaataaa
     2281 aatgttgcaa tcttcaagag catcattccg gagatcgcaa agagaaattt cggtggaatc
     2341 ttacttatcg ttgcaaaccc tgtagatatt ttaacacagg tggcacagaa gctttcagga
     2401 cttccgcagg agcgtgtcat cggttcaggt accgtgcttg atagtgcaag acttaagtat
     2461 gcgctcggag aacaccttgg ggtggatagc cgaagcgtac atgcatttat agtaggtgag
     2521 cacggagaca gtgaggtggt tgcatggtca tcagccaatg tttccggagt ggagcttcac
     2581 aagatgtgcg agatgagagg tcactacaag cataaggaga atacagagga aatcgctgca
     2641 aacgtaaaga acagtgccta cgagataatc aacagaaagc atgcgacata ttatggtatc
     2701 gctatgtcgg tcagacgtat ctgcgaggta attatgcgtg atgagaagtc tattcttcct
     2761 gtttcacaca tgatgcacgg acagtacggc atcgacggtg tggttctctc aatgcctgca
     2821 atcgttggag caaatggagt ggagagcgat atcccaatcg agttaaacgg tgaggaagct
     2881 ttaaagctca aggagtctgc tgatgcactt aagagcatac ttgacgggct tgagctgtaa
     2941 atatcaggct cttaatatta attttgtaat attaaactta tataaaatac tataaattca
     3001 gtagggtttg gggtataatg ataactgtcg ggctgcatat tcttatgtgc ctggcagttc
     3061 ttttagttaa tgacagataa ctttagaact acttagagca aaaaggagta tatataatga
     3121 gcgaaagaaa acaaatccat caggagtttc tgacaggcga aagagccctg tttgcaagca
     3181 atgacctgga tatatatgat acaatttttg atgatggaga gtctcctctt aaggagagcc
     3241 atggcattag cctgacaggc tgttttttta gatggaaata tccgctgtgg tactgcagtg
     3301 acatagctgt aaaggactgc acatggtttg acatggcgcg tgcgggtgtc tggtatacgg
     3361 acaatatctc tgtcactgat tcaatagttg aggcacctaa gaacttcagg cgctgcaata
     3421 acctaaagct taaaaatgtg acttttccaa atgcccagga gacactctgg agctgtgatc
     3481 atgtcgannn nnntatcgag aatgtctcgg caaagggaga ttattttggc atgaatacaa
     3541 ataatcttat catgaagaat ttccagctgg tcggcaacta ctcgtttgac ggctcaagaa
     3601 atgtagaggt gcacgattca aagcttatct caaaggatgc cttctggaat acggaaaatg
     3661 tcacagtcta cgattcattt attacaggtg aataccttgg ctggaacgca aagaatctga
     3721 cacttataaa ctgcacaatc gagagcctgc aggggctttg ctacatcgac aacctcgtta
     3781 tgaaaaactg taagctcata aacactacat tggcatttga gtattcttcg gtagatgcag
     3841 agatttgcag taaggtggac agtgttttaa atccgacaag cggagtgata agggcaaagg
     3901 agattggcat actgactatc gaaaaggaca gagttgaccc atcaaagact aagataatat
     3961 gtgagggaaa ataatctatg tatgattttg acaccgttgt agacagaaga aatacaggct
     4021 cactaaagtg ggatgtggca gaaaatgagc tcccgatgtg ggtagcagat atggatttta
     4081 agacagcacc ccagataata gatgcaatat cagagcgtgt ttctcacgga gtgtttggct
     4141 attccatcat tccggatgag tggaatgatg cgtatatttc gtggtgggac agacgccatg
     4201 ggcttaagat cgaaagagac agcctgattt tttgcacagg ggtcgtaccg gccatatcat
     4261 ctacagtgcg aaagctcaca acacctggtg aaaatgtgct tatcatgaca cccgtataca
     4321 atatattttt taattctata tttaataacg gcgtaaatgt gcaggaaagc ccgcttgggt
     4381 acgaaaacgg cagatatttc gtagactatg acaggctgga gcatgacatt tccaacccac
     4441 agaccacgct tatgatattt tgcaaccctc acaatccgtg cggtatcatc tggacaaagg
     4501 atgagcttgc gaaaataggt gcactctgca ggaagtacaa tgtgacggtc atatcagacg
     4561 agatacactg cgacatcaca gcaccgggaa aatcatacgt gccgtttgcg gcagcatcgg
     4621 aggactgcgc cgatataagc atcacctgta ttgcaccgac caagtgcttt aatatggctg
     4681 gcttacagac tgcggcagtg atggtgccaa ataaattttt gcgccacaaa gtatggcgcg
     4741 cattaaatac agacgaggtg gcggagccaa atgcatttgc catcacggca gcagttgcgg
     4801 cattcacaaa gggtgagccg tggattgagg agctcagaaa atatctctgg gaaaaccgca
     4861 aaacagtctg tgacttcgtg gcagaaaacc tgccgcagat acatgtagtg gatgccgatg
     4921 cgacatacct catgtggctt gactgcagtg cactcacaga tgattctgtg gcatttacgc
     4981 agatgattcg tgaaaagaca ggcctttatc tgtcggaggg agctgagttt ggaggcaatg
     5041 gccgttactt tgtgcgattg aatatcgcat gccccggttc agtgcttatg gaagggcttc
     5101 aaaggttaaa aagagctctc gtttaagttg aatatgtaca ttttctaaaa taaataagac
     5161 cggtgatcag ccggtcttat ctggtataat taaacattta tggcatacaa gatttagaaa
     5221 tatatcatgc atacgtttgt tttctgattc gatagttagt atacactaac atgaattaga
     5281 agtcaataac aaatatgggg ttttgatatt attttggatt tttataaatc acgtacaaat
     5341 caaaaagaaa agttttgcaa acaataaaat gtatgttata ataaaataac tgaattaaaa
     5401 cgaacgaaaa taaactcacg ggggtattcg aaatgcgaaa caatgaatca tcagaaaact
     5461 atttagagac catacttatt ctcagcaaga agcttcctgt ggtacgctca gttgatatag
     5521 caaatgagct tggatttaag aaatccagcg tgagtatagc catgaaaaac cttagagaga
     5581 agaacaatat cacagttagt gatgcaggct ttatcacact cacagagtca ggaagacaga
     5641 tagcagagat gatatacgaa agacatgagc ttttgtcaga gtgtcttgag aagcttggtg
     5701 ttgataagga gattgcagca gaggacgcat gccgtataga gcatgtcata agtcctgaaa
     5761 gctttgaagc aatcaagaag catatacata agagctgatg tatgaaacca gtaccgcagg
     5821 gtactggttt tttagtgtat cgtttttctt gtatatatga gaaaaatcat ctataatagt
     5881 gaagctgtaa tcgttgctgg tcatacagag acgtaaacag taacaaaaat ctggaagggg
     5941 aaaagatgaa acagatatta aagtatttga aggagtacaa aaaagaatgc atctgtgctc
     6001 cgctatttaa gctgctcgag gcatctttcg agcttatagt accgcttgtc atggcagcaa
     6061 tcattgataa cggaatcgcg gcttcggaca agccatatat atggaagatg ggaggtgtgc
     6121 tggtgctgct tgcggcagtc ggcctgataa gctcggtcac agctcagtat tttgcggcca
     6181 aggcggcagt cggcttttca acaaagcttc gtcatatatt gtttgaaaag atagagtcat
     6241 tatcattttc aaagatggat acagtgggca catcgacact tatcacgagg atgacaagcg
     6301 atataaacca ggtacagtca ggagtcaatc tggtgcttcg actcttcctt cgttcgccat
     6361 ttattgtgtt cggagctatg gcgatggctt ttacagtaaa tgtaagagcg gcaatggttt
     6421 ttgtagttac catacctctg ctttcaatag tggtattctc cgttatggct gcaagccttc
     6481 cactttacaa aaaggtgcag agctctcttg atacagtgct ttcnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cacaccaggg agaacttaga gggaaccagg
     6601 gttataagag ctttcaataa gcaaaatgat gagattgaca gcttcaacag tgacaacgag
     6661 cttttaacca atatgcagca ggttgtagga cgaatctcag cactcaccaa tcctctgaca
     6721 tttatcatta taaatattgc aacaattgca gttatcgtat caggcggcaa acaggtatac
     6781 gcaggaatcc tcacacaggg tgaggttgtg gcacttgtaa actacatgtc acagatactt
     6841 gtcgagctga taaagcttgc aaaccttatc gtacaggtga caaaggcagt ggcgtgcggt
     6901 aatcgtatag tggatgtgct ttctattcca tcaaagcttc ctgagaaaaa tcctaagctc
     6961 attgatgcaa aggcaggggc acctgaggtt gagtttgacc atgtctgcat gacctatgag
     7021 ggagcggccg atgagacact cacagatata agctttacag tgcaaaaggg gcagacaatc
     7081 ggaatcatcg gtggtacagg ttcaggaaaa tcaagccttg taaaccttat cccgaggttt
     7141 tatgatgccg caaagggtac tataaggatt cagggaaatg atataaatga ctacgatgca
     7201 gtgcagcttc gagataaaat cggtgttgta atgcaaaagg cagtgctttt tgcaggaaca
     7261 atagccgaca acctgcgctg gggcaaaaat gatgccacag aggaggaaat gtggaaggcn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn atgcatggtg acaagacaat atttatcgtg tcacagcgta cctcatctat
     7561 ccagtttgcg gacaatatca tagtgctcga cgacggacag atggttggct tcggaccgca
     7621 tgaaaagctg cttgagacat gcgagaccta caaagagata tatgattcac agtttaaaaa
     7681 agagtcggat atgactaaac aaaaggaggt gtagagtatg gctatggata atgcgaaaat
     7741 gaataaagca ggtacaatgc gcaaggtact aagctacatg aagagatata ttcctttgct
     7801 tgtaatttcc cttgccctgt ctgttgttac agtggcgctt acgctttatt ttccaatcct
     7861 cacaggacgc gcaattgatt tgattgtagg aaaagaaaag gttgatttta cggctatgac
     7921 agccatcctc acaagagctg caataatagt cgtcatagcg gcagcggccc agtggcttac
     7981 aaatatctgc aacaacagga tgacctacaa catagtgaag gatataaggc atgatgcatt
     8041 tttaaacatt gaaaaaatgc cactttccta tattgacagc cactcacacg gtgatatggt
     8101 cagccgtatc atcgccgatg tagatacatt ttcagaggga cttttgatgg gcttcacaca
     8161 gctctttaca ggaattgcaa caatagcagg
//
LOCUS       DPUF01000216            3419 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_52060, whole
            genome shotgun sequence.
ACCESSION   DPUF01000216 DPUF01000000
VERSION     DPUF01000216.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_52060"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="DHV81_07670"
     CDS             <1..399
                     /locus_tag="DHV81_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCI94325.1"
                     /translation="DMVSGRVYDFSDNFGTFIAVDDKYSAKIPVFENAAYLKLGDVIE
                     AKVTDVKADGKLDLTMREKAYIQMDSDAEKLLELLDSYAGVLPFSEKASPEVIKRETG
                     LSKNAFKRAVGHLYKERKIDLTDGKIRLKK"
     gene            442..816
                     /locus_tag="DHV81_07675"
     CDS             442..816
                     /locus_tag="DHV81_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCI94326.1"
                     /translation="MKNVISTDKAPAAIGPYSQAIEVNGMVYTSGVIPVVPETGEIPE
                     GSQAQAKQAFTNLSHLLEAAGTSMDNVVKTTVFIKEMNDFGAINEVYATFFNGAYPAR
                     SCVEVARLPKDVMLEVEAIAVK"
     gene            993..2228
                     /locus_tag="DHV81_07680"
     CDS             993..2228
                     /locus_tag="DHV81_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase Nlp/P60"
                     /protein_id="HCI94327.1"
                     /translation="MKVNYVKLTAGVLTGFMALSSVPYAVYAAGPTAGVASYTSDIVS
                     TSNTLTAGVTSMLTDVMLKGDETSETATDVATTDAEPVKSAYADTAVAQVSDSVNIRA
                     SADENSESLGKLYNNGIGTVLETTDNGWYKIQSGSVTGYVKGDYVVVGDDALVQSAGR
                     RVATVNTETLKVRTTASTDAEVLGLVSGEDDLTVVDESTAGWVGVSTADGTGYVSTDY
                     VTLDTEFTYAESKEEEAARLAKEEAERKAAEEAARKAEEKARKAEEAKAAKAAAKKSS
                     KSSSKSSSSSSSEKSYSAPSGSNGQAVVNYASQFVGNPYVYGGSSLTNGTDCSGFVMS
                     VYAQFGISLPHSSSAMRSVGYGVSTSDMQPGDIICYSGHVAIYCGGNTIVHASNPSDG
                     IKYTSPANYKSIIAVRRIF"
     assembly_gap    2310..2419
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2817..>3419
                     /locus_tag="DHV81_07685"
     CDS             2817..>3419
                     /locus_tag="DHV81_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein"
                     /protein_id="HCI94328.1"
                     /translation="MRVDRRISEKILTTIEEVNMDTLALAESDEEVKQALEACDYTVI
                     ADEGILRAASADTLQRRHEIEDHDFFYELFKRLERNDKKIFVIAESQKAVDEAEEFLL
                     GLFDRARISGKGVLDDSPGCSENLVNEINIVSPDAIASFLPSPSQEKFLLHNREKLLM
                     NLWYGIGNNKFMGKKHGFIGKIRKMLDVKRLTHLINTYEHR"
BASE COUNT         1090 a          569 c          791 g          859 t
ORIGIN      
        1 gatatggttt caggccgtgt gtatgatttt agcgacaatt tcggtacatt tatcgctgtg
       61 gatgacaaat attcagcaaa gataccggtt tttgagaatg ccgcttatct gaagcttgga
      121 gatgtgatag aggcaaaggt cactgatgtc aaggcggatg gcaagctgga cctgacaatg
      181 agggaaaagg catatattca gatggattcc gacgctgaaa agctccttga actgcttgat
      241 tcatacgcag gggtgcttcc gttttcggaa aaggcaagcc cggaggtaat caagagagaa
      301 acaggactta gcaaaaatgc attcaagcgt gcagttggtc atttatataa ggaaagaaag
      361 attgatctca cagatggcaa aatccgtctt aagaaataac agtcttttta ataaatttta
      421 acaggaaaga agggcaataa catgaaaaac gttatcagta cagacaaggc accggcagca
      481 atcggaccat attcacaggc tatcgaggta aatggtatgg tatatacttc cggagtgata
      541 ccggtagttc cggagaccgg tgagattcct gagggctcac aggcacaggc taagcaggca
      601 tttacaaatc tgtcacatct gcttgaggca gcaggaacaa gcatggacaa tgtggtaaag
      661 actacagttt tcatcaagga gatgaatgac ttcggtgcaa taaatgaggt atatgccaca
      721 ttttttaacg gagcataccc tgcaagaagc tgtgttgagg tcgcaaggct tccaaaggat
      781 gtaatgcttg aggttgaggc tattgctgta aaataaaatt attacataag tatttcatgc
      841 gacataagtc ctattcgttg gaaaaagtca agattttctt aagaattcac taagaaaacg
      901 ttgacatata tatgttgctt tgttataatg acaaagtaat atttgtaaca aattcgtaat
      961 aactgttaca aattcataca acggagggta aaatgaaagt aaattatgtg aaactcacag
     1021 ccggggtact tacaggtttt atggctctgt catcagtgcc atatgcagta tatgcggctg
     1081 gacctactgc aggagtagct agttatacat cagatattgt tagtacatca aacactctta
     1141 cagccggtgt tacatctatg ttgacagatg taatgttaaa aggtgatgag acatcagaga
     1201 ctgcaacaga tgttgcgaca acagatgctg agccggttaa atcagcatat gctgatacag
     1261 cagttgcaca ggttagcgac agcgttaata tacgtgcgag tgcagatgaa aacagcgagt
     1321 ctcttggaaa gctctataac aatggaattg gaacagtact tgagacaacc gataacggat
     1381 ggtacaaaat ccagtccggt tcagtaacag gatatgtaaa aggcgattat gttgtagttg
     1441 gtgacgatgc acttgtacag agcgcaggac gtcgcgtggc aacagtcaat actgagacac
     1501 tcaaggtcag aacaacagca tctactgatg cagaagtact tggtctcgtg tccggagagg
     1561 atgatctcac agttgtggat gagtcaacag ctggatgggt aggtgtatct acagcagacg
     1621 gtaccggata tgtatctaca gattatgtga cactcgatac agagttcact tatgctgagt
     1681 ctaaggaaga ggaagcagca agacttgcca aggaagaggc agagcgtaag gcggctgagg
     1741 aagcagccag aaaggctgaa gagaaagcta gaaaggctga ggaagcaaaa gcagccaagg
     1801 cagcagcaaa gaagtcatct aagtcttcaa gcaaatcatc aagcagttct tcatcagaga
     1861 agagctatag cgcaccaagt ggttcaaacg gacaggctgt tgttaattat gcatcacagt
     1921 tcgttggcaa tccttatgtt tacggtggct caagtcttac aaacggaaca gattgttcag
     1981 gatttgtaat gtcagtttat gcacagtttg gcatcagctt accacacagc tcatcagcaa
     2041 tgagaagcgt tggatacggt gtgagcacaa gcgatatgca gcctggagat atcatctgct
     2101 atagcggaca tgttgctatc tactgtggtg gaaacactat tgtacatgca agcaacccat
     2161 cagacggtat caaatataca tcaccggcta actacaaatc aatcattgca gttagaagaa
     2221 ttttctaata aattcatgcc ggataggtaa atatgaatct ataaagacct tcatctcatc
     2281 gagatggagg tcttttttac atagagaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnna catatatcaa aatagcaaca tgcacaaaaa agtggatatg
     2461 ctaaacccgg taaattcgga ttggggataa atgtggataa ctatcaacaa aagtaaacct
     2521 caaagtgccc ttaaaatgag ttatgcacgg agttatgcac attatccaca tttttgaatg
     2581 agagcaaaag aacaactgtt ttgtaaagtt ctgataaagt tgcactaaat tagtaatatt
     2641 tcggaaataa tattgacatt ttaattgtaa ataatatgat taaaatgctt tataattgtc
     2701 ggacaacttg aaaagtgttg attatgtaag ttaattttgt tatacttcaa attatgatta
     2761 aaaagataga tattgcaggg ctacaacttg ataactatac agtacgcgag atgctcatga
     2821 gagtcgacag gcgtatttca gagaagatac tcactacaat agaggaagtg aatatggata
     2881 cgctggcatt ggcggagtct gatgaagagg tgaagcaggc gctggaggca tgcgattata
     2941 ctgtcattgc ggatgaaggc atactaaggg cggcatcggc agataccttg cagcgtcggc
     3001 atgagataga agatcatgac ttcttttatg agctttttaa gcggcttgag agaaatgata
     3061 aaaaaatctt tgtgattgca gagtcgcaga aagctgttga tgaggctgag gaatttctgc
     3121 ttggattatt tgacagagcg cgaatatccg gcaagggtgt tcttgatgac tcaccgggat
     3181 gcagcgaaaa tcttgtcaat gaaattaaca tcgtttcacc tgatgcgatt gcatcatttc
     3241 ttccgtctcc gtcacaggag aagttccttt tacacaacag ggaaaagctg ttgatgaacc
     3301 tctggtacgg aatcggaaat aataagttca tggggaaaaa gcatggattt attggaaaaa
     3361 taagaaaaat gcttgatgtg aagcgcctta cgcatcttat caacacctat gaacataga
//
LOCUS       DPUF01000217            3081 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1229, whole
            genome shotgun sequence.
ACCESSION   DPUF01000217 DPUF01000000
VERSION     DPUF01000217.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1229"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="DHV81_07690"
     CDS             <1..780
                     /locus_tag="DHV81_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCI94329.1"
                     /translation="SISAYAGQAAFFLMLSFFPFLLFFFSLLSWTPLTEADFLMWASS
                     FIPESFGDIMKDLASEVYASGTGRLSITMITAVYLSSKAFISFQLGLDDMYHVKENRN
                     FILRRIYSVLYSIVFALALIFLLGIMVFGNMLRKMYFSNVHHIIGSIIGSVVDYRLVI
                     CLPVLILFFWVIYVFLPNKKQRAKYQLPGAVFAAAAWMIFSGIFSVYVDKYNNYSSFY
                     GTMTTIALIMVWLYGCMYVLFIGGIINRTWEERMEAKKYYK"
     gene            803..1759
                     /locus_tag="DHV81_07695"
     CDS             803..1759
                     /locus_tag="DHV81_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HCI94330.1"
                     /translation="MDNNKEMIEGLVSQILDDYHDDKIINQQALFSQPDKEAVCDLLS
                     KLIKIMYPGYFKGTSFRYYNLDSQIAVLLEDIMYNLRKQVVLALPQNPKYRSLSHHEL
                     VNMSEKISNDFFKRIPKIRALVETDVAAFFDGDPAAYNYNEIILCYPGLLAITTNRIA
                     HELHLLGVPLIPRMMTEHAHSKTGIDIHPGATIGKNFFIDHGTGIVVGETTIIGDNVK
                     VYQGVTIGALSTRGGQKLKGVKRHPTIEDNVIIYAGASILGGETVIGRGAVIGSNAFI
                     TSSIEPGARVSIKNQELSIRTGERKELVASEMETDSSWFYVI"
     gene            2515..2727
                     /locus_tag="DHV81_07700"
     CDS             2515..2727
                     /locus_tag="DHV81_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCI94331.1"
                     /translation="MMPLSMSGAGEAQIIKKIGGKEEVRSFLEKLGFVVGTEVTVISE
                     IAGNMIVNVKGARVAIGKDMANKIMV"
     gene            2918..>3081
                     /locus_tag="DHV81_07705"
     CDS             2918..>3081
                     /locus_tag="DHV81_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCI94332.1"
                     /translation="MKTLKQAKVGETVNVVKLTGQGAVKRRIMDMGITKGVEIFIRKV
                     APLGDPVEVTV"
BASE COUNT          899 a          574 c          748 g          860 t
ORIGIN      
        1 tctatttctg catacgcagg gcaggcagcc ttctttttga tgctgtcgtt tttcccgttt
       61 ttattgtttt tcttttcgct gctgagctgg acaccgctta cagaggctga ttttttgatg
      121 tgggcgagct cgtttattcc ggagtcgttt ggtgacatca tgaaggatct ggccagtgag
      181 gtgtatgcaa gcggcacagg caggctttct atcacgatga ttacagccgt gtacttaagc
      241 tcaaaggctt ttatttcgtt tcagcttgga cttgatgata tgtaccatgt gaaggaaaac
      301 agaaatttta ttttgcggcg catatattcg gtgctttaca gtatcgtttt tgcactggcg
      361 ctcatttttt tactgggtat catggtgttt ggaaatatgc tcaggaagat gtatttttcg
      421 aatgttcatc atataattgg aagtataata ggcagtgtgg tcgattatcg tctggtaatc
      481 tgcctgccgg tgcttatttt atttttctgg gtgatttatg tgtttctgcc aaacaagaag
      541 cagagggcaa agtaccagct gccgggagcg gtatttgcgg cggctgcatg gatgattttt
      601 tcgggaatct tttctgtgta cgtggataaa tacaataatt attcttcatt ttatggcaca
      661 atgaccacta ttgcgcttat aatggtatgg ttatatggct gtatgtatgt gcttttcata
      721 ggcggtataa taaaccgcac atgggaggag cgtatggagg caaaaaaata ttataaatag
      781 taaattcgga ggcttcgaat atatggataa taataaagaa atgatagaag ggcttgtatc
      841 acagatactt gacgattatc atgatgacaa aatcataaat cagcaggcac tattttcaca
      901 gccagacaag gaggctgttt gtgatctgct ctcaaagctt attaagataa tgtatccggg
      961 ctattttaag ggcacgagct tcagatatta caacctggac agccagatcg ctgttctttt
     1021 ggaggatatc atgtacaatc tgcgcaagca ggtagtgctt gccctgccgc agaatcctaa
     1081 atacaggagc ttatcacatc acgagcttgt caatatgtct gagaaaatca gcaatgattt
     1141 ctttaaaagg attccaaaaa tcagggcttt ggtggagact gatgtggctg cgttttttga
     1201 cggagaccct gcagcctaca attacaatga gattatcctt tgctatccgg gactgcttgc
     1261 aatcactaca aaccgtatag cccacgagct tcatctgctt ggagtaccgc tcatcccgcg
     1321 tatgatgaca gagcatgcac acagcaagac cggtattgat attcatccgg gtgccactat
     1381 tggcaagaat ttctttatag accacggaac aggcatcgtg gtcggtgaga ctactattat
     1441 cggtgacaat gtcaaggtat atcagggtgt gactatcgga gcactttcta ccagaggcgg
     1501 tcagaagcta aagggcgtga agagacatcc tactattgag gataatgtca tcatctatgc
     1561 cggagcttct atacttggtg gtgagactgt catcggaaga ggtgctgtca tcggaagtaa
     1621 tgcatttatt acctcatcta tcgagccggg ggcccgtgtg agcatcaaaa accaggagct
     1681 ttccatcaga acaggagagc gaaaggagct tgtggccagc gagatggaga cggattcgag
     1741 ctggttttac gtaatataat attataatat atttttttga ctgaggttcg gtacgcgtgc
     1801 cccgggcact aggtgtcgca cacgtactct cctgcaggct acgctttaca gttaagatac
     1861 tgtatgaatt atgcttttta gggtttctat gtttgacgaa acagtcgaaa cgcgccgtcc
     1921 atggcgcggt tctcctcgct cctgcgtcct tgcaggagcg tttctgcgat ttgtgggcat
     1981 aattctaaca gtatctaaaa tgtttcgcta tgcgcctgca ggagagtacg tgtgcgccac
     2041 cggtgcccgg gacgcgcgta ccgacattca gtcaaaaaag tatatttatt aagtaggact
     2101 aaattagcat aatgtacaat agccataggt tgatagcata gtaggcaggc ccttgtggag
     2161 cggtaatcca taaaactttt aaaacagctc cccaacccag cccataagcc agaaaaattg
     2221 cgtgaaaaag aagctgaaat tgcagaaaat cccaacaagc ataatttcgt acagtatctt
     2281 agcagcgaag cgtagcgaac gcgagagtac taagatggtg agactccgcg agcaagcccg
     2341 atggtaccga gttttgggac ccaccctatt agccacaact gaaaaaagta agaaaaatct
     2401 aaaaaatatt attgacatct aagtaacgtt agagtatact aacacccgta agaggtacca
     2461 aatgatgttt tttatcatta aagacgtgaa gaaaagacgt gaaaagaggt aaatatgatg
     2521 ccattatcaa tgagtggtgc tggggaggca cagataatca agaaaatcgg cggaaaggaa
     2581 gaggtgagat ctttccttga gaagctgggc tttgtagtag gcacagaggt gactgtcatc
     2641 tcagagatag ccggcaatat gatagtcaat gtaaagggcg ccagagtagc aatcggcaag
     2701 gacatggcaa ataagatcat ggtttgatat aacatatgta acaggcatgg atatagcatg
     2761 tatgatatat ataacatata taacacatat aacacatgca gcaatgtgct gtataagctg
     2821 taatcattta agatttaaac gactttgtat aagatattat gcaaacgttt atataaattt
     2881 tttaaacaac aataaaatta aaaaaggagt agtcagcatg aagacattaa agcaagccaa
     2941 agtaggcgag acagtcaatg ttgtcaagct cacaggccag ggcgcagtca agagacgtat
     3001 catggatatg ggaattacta agggtgttga gatatttatc cgcaaggtgg caccgcttgg
     3061 cgatccggta gaggtgacag t
//
LOCUS       DPUF01000218            4372 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_11355, whole
            genome shotgun sequence.
ACCESSION   DPUF01000218 DPUF01000000
VERSION     DPUF01000218.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_11355"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(36..>989)
                     /locus_tag="DHV81_07710"
     CDS             complement(36..>989)
                     /locus_tag="DHV81_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCI94333.1"
                     /translation="ALYDADDETFIYGKNMRDSMANASTTKLLTCIVALEKADINDTV
                     TISQNAASQPAVKLGLVAGNSYNMKDLLYGMMLESFNDCAYAIAEHVGGSTEGFAKLM
                     NEKANEIGCLGTYFITPNGLDAENDISFHHSTAGDLCVIMSYCAWKSPKSTQFLEITQ
                     TMQYTFETEEGKMVFNNHNRLITETDYCISGKTGFTTKAGYCYVAAVEKDGRRMCLSL
                     LGCGWPNNKNYKWADAKSLVEYAVSDAAEDSYCDAACVTTQQTGDTQTTDKQAAGKET
                     PAVKKEDRKTINLKYIENNKKNKKICKNFLKNFTINIGNFF"
     assembly_gap    990..1207
                     /estimated_length=218
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1292..1855)
                     /gene="scpB"
                     /locus_tag="DHV81_07715"
     CDS             complement(1292..1855)
                     /gene="scpB"
                     /locus_tag="DHV81_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCI94334.1"
                     /translation="MNYKAAIEAILFTMGESVELGRIADAIQLNEKETKKLLDELIEE
                     YRSSSNIGMNIIELDGAYQMCTKPQMYEYLIRIAKQPKKRVLTDVLLETLSIIAYKQP
                     VTKAEIEKIRGVSSEHAVSKLVEYNLVQELGRLDAPGRPLLFGTTEEFLRSFGVSSID
                     ELPVLSPVQVEEFKQEAEEEMHVKLDV"
     gene            complement(1873..>2059)
                     /locus_tag="DHV81_07720"
     CDS             complement(1873..>2059)
                     /locus_tag="DHV81_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCI94335.1"
                     /translation="LTDYAASHKHFSFRGMLTASSSKVEVIVTFLAILELMKTGKLTI
                     VQEHIFDDIRIESRIAA"
     assembly_gap    2060..2213
                     /estimated_length=154
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2214..2629)
                     /locus_tag="DHV81_07725"
     CDS             complement(<2214..2629)
                     /locus_tag="DHV81_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCI94336.1"
                     /translation="MDMTFKLQVFEGPLDLLLYLIEKNKVNIYDIPIVEITAQYMEYV
                     NQMKKDNLDTLSEFLVMAATLLDIKSKMLLPKKEEEEQEQEDPRAELVQQLLEYKMYK
                     CMANELKDRQLDAGKVWYKKKDIPDEVLAYEEPVNL"
     gene            complement(2643..3836)
                     /locus_tag="DHV81_07730"
     CDS             complement(2643..3836)
                     /locus_tag="DHV81_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCI94337.1"
                     /translation="MKNLEIYIHIPFCVRKCEYCDFLSFPADDDAKLRYVKGLMAEMI
                     FYGPLMKNYQVSSVYIGGGTPSSIDEQWIAALMEGVFDCFNVLPDAEISIECNPGTLS
                     REKLLAYRDAGINRLSIGLQSANNDELKLLGRIHTFEQFVTNYEVARNVGFKNINVDL
                     MYGLPGQSASQYMATVNKIIRLHPDHISAYSLIVEKGTPFYEKYKFDMVRQEAGMRTE
                     FLPNEDELYDMEKAGQKAFMDAGYRQYETSNYAKRGMECRHNIGYWTRADYLGLGIGA
                     ASLISNVRYTNISDMDEYLSRCRHIHDVGDDIFKANLHVAADVIDKKGQMEEFMFLGL
                     RMNEGVTREAFERAFGISIDAIYKDTIDSLKKQELLVVKAGHIYLSERGKDVANYVMA
                     QFLKD"
     gene            complement(3845..>4372)
                     /locus_tag="DHV81_07735"
     CDS             complement(3845..>4372)
                     /locus_tag="DHV81_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498824.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCI94338.1"
                     /translation="ERRGVYISMEYIEKTRALIKYDLPLNEIIYDFFDALKSRSRGYA
                     SFDYEMKDYERSELVKLDILINKEMVDALSFIVFKESAYERGRKMCEKLKGEIPRHLF
                     EIPIQAAVGGKIIARETVKALRKDVLAKCYGGDISRKKKLLEKQKEGKKRMRQVGNVE
                     IPQEAFMSVLKLDEE"
BASE COUNT         1037 a         1044 c          663 g         1256 t
ORIGIN      
        1 aactaatttg ttaaattttt aactttaatt tttgtttaga aaaaatttcc tatattaatt
       61 gtaaaattct tgagaaaatt cttgcaaatt ttcttattct tcttgttatt ttctatatat
      121 tttaagttaa ttgtttttct atcctctttt tttaccgccg gagtttcttt tcctgctgcc
      181 tgtttatccg ttgtctgtgt atcaccggtc tgttgtgttg ttacacaagc agcatcacag
      241 taagaatctt cggcagcatc tgaaaccgca tactccacca gactctttgc atcagcccac
      301 ttatagtttt tattgtttgg ccacccacag cccaagagag aaaggcacat tctccttcca
      361 tccttttcaa ctgctgcgac atagcagtag cctgcctttg tggtaaagcc ggtctttccc
      421 gatatgcagt agtccgtctc cgtaatgagc ctgttgtgat tgttaaaaac catcttacct
      481 tcctctgtct caaacgtgta ctgcatagtc tgtgttatct caagaaactg tgttgatttt
      541 ggtgatttcc acgcacaata agacattata acacacaaat ctcccgctgt ggagtggtga
      601 aagcttatat cattctcagc atccagcccg tttggtgtaa taaaatacgt gccaaggcac
      661 cctatctcat ttgctttttc attcataagc tttgcaaagc cctccgtcga gccgcccaca
      721 tgctccgcaa tcgcataggc acagtcattg aacgactcaa gcatcatgcc atagagtaaa
      781 tccttcatat tatagctatt ccctgccaca agtccaagct tgaccgcagg ctgtgatgcg
      841 gcattttgtg aaatcgtaac agtatcattt atatcagctt tttcaagtgc cacaatgcac
      901 gtaagaagct tcgtggtgct ggcatttgcc atgctgtctc tcatgttttt accatatata
      961 aacgtctcgt catccgcatc atataatgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnncat ataattacct ccatcggtaa tatatatgat tatttgcatg tccttattat
     1261 atgaaatggc agcttacgca atacacctgc actacacatc aagcttcacg tgcatctctt
     1321 cctctgcctc ctgcttaaac tcctcaacct gcacagggct tagtaccggc agctcgtcga
     1381 tagacgaaac cccaaagctc cttagaaatt cctctgtggt accaaacaaa agcggtcttc
     1441 ccggagcatc aagccttcca agctcctgca ccagattgta ctcgacaagc ttgcttacgg
     1501 catgttcact tgacacacct ctgatttttt ctatctctgc ctttgttaca ggctgcttgt
     1561 acgctatgat tgaaagagtc tctaaaagca catcagtaag caccctcttc ttaggctgct
     1621 ttgcaatgcg gatgagatat tcatacatct gtggctttgt acacatctga taagcaccat
     1681 caagctctat gatattcata cctatatttg aagagcttct atactcctca atcagctcgt
     1741 caagcagctt ttttgtttct ttttcattta attgtattgc atcggctatc cttccaagct
     1801 ccacagattc tcccatggta aataaaatag cctctattgc agctttataa ttcatatatc
     1861 ctcaaaccct ctttatgcag caatccttga ctcaatccgg atatcatcaa aaatatgctc
     1921 ctgaacgata gttaatttgc cggtcttcat aagctccagt atggcaagaa atgtaactat
     1981 gacttccact ttgcttgacg atgcagtaag catccctctg aagctgaagt gcttgtgtga
     2041 ggcagcatag tctgtcagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcaagat
     2221 taacaggctc ctcatatgca aggacctcat caggtatgtc cttcttctta taccacacct
     2281 tgccggcatc cagctggcga tccttaagct catttgccat gcacttgtac atcttgtact
     2341 caagcagctg ctgcacaagc tctgctcttg gatcttcctg ctcctgctct tcttcctctt
     2401 tctttgggag aagcatcttt gactttatgt cgaggagcgt agctgccatc acgagaaact
     2461 cgctgagcgt gtccaggtta tcctttttca tctgattcac atactccatg tactgcgctg
     2521 taatctcgac aatcgggata tcataaatat ttactttatt tttctctatc aggtataaaa
     2581 gcaggtcaag cggtccctca aacacctgaa gcttaaaagt catatccata atttgctctc
     2641 cgttaatcct tcaaaaactg tgccataacg taattggcta catcctttcc tctctccgat
     2701 aaatagatat gcccggcctt taccacgagc agctcctgtt ttttcaggct gtctatcgta
     2761 tccttgtaga ttgcatctat cgaaatacca aatgccctct caaaggcttc tcttgtgacc
     2821 ccctcattca tacgaagtcc aagaaacata aactcctcca tctgtccctt tttgtctata
     2881 acatcagctg ccacatgcag attggcctta aatatatcat cacccacatc atgtatatga
     2941 cggcagcgcg acagatattc gtccatatcg gatatattgg tatatctgac atttgatata
     3001 agcgatgccg cccctattcc aagtcctaaa tagtcagctc tggtccagta acctatatta
     3061 tggcggcact ccatacctct tttagcatag ttagatgtct catattgcct gtagccggca
     3121 tccataaacg ccttctgtcc cgccttttcc atatcatata actcgtcctc attaggcaga
     3181 aactccgttc tcatgcccgc ctcctggcgc accatgtcaa acttgtactt ttcatagaaa
     3241 ggagtaccct tttccactat aagagaatac gccgatatat gatccggatg aagccttata
     3301 atcttgttta ctgtcgccat atactgtgac gctgactgtc ccggcagacc atacatcaaa
     3361 tctacattga tatttttaaa gcccacgttt ctggccacct cgtaattcgt cacaaactgt
     3421 tcaaaagtat gtatacgccc gagcagctta agctcatcat tatttgccga ctgcagtcct
     3481 atggacaggc ggttgattcc cgcatccctg tatgctaaaa gcttttccct gctaagcgta
     3541 ccgggattgc actctatgga tatctcagca tcaggcagca cattaaagca gtcaaacacg
     3601 ccctccatca aagcagcaat ccactgctca tctatagagc ttggtgtacc tcctccgata
     3661 tagacagatg acacctgata gttcttcata agcgggccgt aaaatatcat ttccgccata
     3721 agccccttga catagcgaag ctttgcatca tcgtctgcgg gaaatgataa aaaatcacaa
     3781 tattcacatt ttctcacaca aaatggtata tgaatataaa tctcaagatt cttcatatgt
     3841 gcgattactc ctcatccagc ttaagcacac tcatgaatgc ctcctgtggt atctctacat
     3901 tgcccacctg acgcatcctc ttctttcctt ccttctgctt ctcaagcagc ttcttcttac
     3961 gtgaaatatc accaccgtag cacttagcaa gcacatcctt gcgaagtgcc ttaaccgtct
     4021 ctcttgctat aatcttgcca ccgactgctg cctgaatagg tatctcaaac aaatgccttg
     4081 gtatctcacc cttaagcttc tcgcacatct tacggcctct ttcgtatgcg ctctccttga
     4141 atacgataaa tgaaagtgca tcaaccattt ccttattgat taagatgtca agcttgacaa
     4201 gctctgagcg ctcataatcc ttcatctcat agtcaaacga agcataaccc ctcgagcgtg
     4261 actttaaagc atcaaagaaa tcatagataa tctcatttaa tggcaagtcg tactttatga
     4321 gagctcgtgt tttttcgata tactccatac tgatatagac tcctcgtctc tc
//
LOCUS       DPUF01000219            4904 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23676, whole
            genome shotgun sequence.
ACCESSION   DPUF01000219 DPUF01000000
VERSION     DPUF01000219.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23676"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="DHV81_07740"
     CDS             complement(<1..896)
                     /locus_tag="DHV81_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCI94339.1"
                     /translation="MKKKLIATVMAATMAVTALTGCGSSSASSAKKDIKVPTKPFGDT
                     VKYDPSVEINDGKDISIDLWEWGSDELFQKVIDRYTAIHPNVTINLVDNPWEDYWTKL
                     PLALDGEDGPALFDVHNSYHENLINYMAPLDIPVEDLEQDFNGVSAHVIDGKVYYIDY
                     GMMTGSVYYNKEMWKEAGLTDDDIPKTWDEMIEVAKKLTIKDGDNIVQAGLNFNNDFN
                     QNYLLGLNYQLGENLFKEDGKTPNVNSDAMKKVMQMLVDMYDKDQIGSKDFGDKCADS
                     FGQGQSAMVIQWGHYYNTLKTT"
     gene            1265..2290
                     /locus_tag="DHV81_07745"
     CDS             1265..2290
                     /locus_tag="DHV81_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HCI94340.1"
                     /translation="MTLKDIAEMAGVSTMTVSNVINGKTSRVSQKTRDKINSIIEEYN
                     YVPNMNARSLSNNSSHIIGVVTFLEEKEYASGYNYFENPYVSTMIGTIETELHKNGYF
                     TMLQSVSRSSDILSLVKNWNVDGMILVFPTSAQNLEKLMDAANCPIATFDSNYSHPNL
                     INVISDDEKGLYLSTKYMINHGHTEIAFVADYEGNIVLTKRFNGYKKALEDSHIPFRP
                     EYIFSYSPSYEGGVEAGRAIASSKAPITAVVTTADICAIGIMEGARLGGYRVPIDLSV
                     IGYDNLNLCQYTVPKLTSVSQNVPKKARLATELLLKKIHDNEADCNFGEIMDVEIVDR
                     QSVISLY"
     gene            complement(2287..3423)
                     /locus_tag="DHV81_07750"
     CDS             complement(2287..3423)
                     /locus_tag="DHV81_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94341.1"
                     /translation="MQTLKLLFSKITDDNPIYEKKDVVENIFVFRILLIWHLLYTFVF
                     ILNCLNIFIVDNNIFVKGYILSCILLLVFIVLLMIVGLEQACAKYLCISEAGFLVMIT
                     CSSLTYHMIIAVMLPIVIAGVYSSKHLSRFALAFSIFNIALSTYVGYYFGVCDANMVL
                     LTATSLEHLQKDGVFLLNEVNKNPGFTLFMYYVFPRSLIAAGFYSVSNGVNKMLRRTM
                     EKAHRIKKEAAMDDMTGLYNKNKLLDDLEKPKEPDRKVAVIYWDVNQLKFVNDTYGHG
                     KGDRLIARIAGTIRLAAANDNDNVRVYRYGGDEFIMIIPDGTEDDALSFIERWNKIME
                     PIQKDSKIPISASVGYACGRYADINKIICEADEKMYENKRVRRD"
     gene            complement(3454..4803)
                     /locus_tag="DHV81_07755"
     CDS             complement(3454..4803)
                     /locus_tag="DHV81_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI94342.1"
                     /translation="MKTIEFDTHFYKKLWTLMVPIMVQNLMLALVAVADAFMLGGLDQ
                     NYMSAVSLATQIQFIQNMFLSAATAGLAILGAQYWGKQDIKALDDIFALAIRICGIVS
                     VLFFVACAFFPRYLMLIFTNEPVLIDYGVSYLKIASFSYLLTGFSQCYIVMMKISEHA
                     GTAAAVSSITVLINIVLNAVFIYGAFGIESMNVRGAALATLISRVVELMLSITFSYRP
                     GYIRLKIRELFARNRELSADFMKCSGPILGASLVWGIGFTSYSTFMGHMGTDAAAANS
                     VAAVVRDIICCLSAGISSAAGIMIGNELGAGNLKRGKTYGIRMAKLSFLCGAVMTVLM
                     AVSAPVILHFVKLTPQAAGYLKQLFGVLAFYMIGRSVNEIIINGVFGSGGDTMFDMYS
                     LAVTMWGIAIPLAVAGTYFLNWPVVVVYACTCVDEIGKIPWTLIHFKKYKWVKDLTR"
     gene            complement(4833..>4904)
                     /locus_tag="DHV81_07760"
     CDS             complement(4833..>4904)
                     /locus_tag="DHV81_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HCI94343.1"
                     /translation="SPANAAWTFERLCEAWTRILVNL"
BASE COUNT         1456 a         1136 c          845 g         1467 t
ORIGIN      
        1 catgtggtct taagtgtatt gtaataatgt ccccactgaa taaccatcgc cgactgtccc
       61 tgtccaaagc tgtctgcgca tttatcgcca aaatcctttg aaccaatctg atccttatcg
      121 tacatatcaa caagcatctg cattaccttc ttcatggcat ccgaattgac attaggtgtc
      181 tttccatcct ccttaaagag gttctcacca agctgataat taagaccaag cagataattc
      241 tggttgaaat cattattgaa attgagtccg gcctggacta tattgtctcc atccttgatt
      301 gtgagcttct ttgccacctc aatcatttca tcccaggtct ttggaatatc atcatctgtt
      361 aatccggctt ccttccacat ctccttgttg taataaacag agcctgtcat cattccgtaa
      421 tcaatgtaat aaaccttacc atcaatcaca tgagcgctca ctccattgaa gtcctgctcc
      481 aaatcctcaa caggaatatc aagcggagcc atatagttga taagattctc atgatagctg
      541 ttatgtacat cgaagagtgc cggtccgtcc tcaccatcga gtgccagagg aagctttgtc
      601 cagtaatctt cccatggatt atccaccagg tttatagtta cattaggatg aatagctgtg
      661 tatctgtcaa taaccttctg aaataattca tcgcttcccc actcccacag gtcaattgag
      721 atatcctttc catcattaat ctcaaccgaa gggtcatact tcaccgtatc gccaaatggc
      781 tttgttggta cctttatgtc ctttttagct gatgaagccg atgagctgcc acagcctgta
      841 agcgctgtca ctgccattgt agctgccata actgttgcaa taagtttctt cttcatagaa
      901 atcctcactt tcttaattca atatcctctt tttcgttcaa attagtttag cgctaaatct
      961 aactataatc taccaccacc ttatacattc gtcaatagtt ttttgttttt tatcactttc
     1021 catattattt tcatatgttt tttgtgcatt tttacatgta aattagggtt ttgtctaaat
     1081 atttgcaaaa accgattgct atttgcaaaa taaaaatgta tacttaagat aatttataaa
     1141 cccatatttt gaatcatatt cggtttatgt ataaactcat cattgtttat gttcaaactt
     1201 attgtggttt atgtataaac tttatagtaa aaaaattata atactgaata gaatggagtt
     1261 taatatgacg cttaaggata ttgccgaaat ggcaggtgtg agcacaatga ccgtctcaaa
     1321 cgtaatcaac ggaaagacaa gccgtgtttc acagaaaaca agggataaaa tcaattcaat
     1381 tatagaggaa tataattatg ttccgaatat gaacgcaaga agtctttcaa acaattcctc
     1441 acacattatc ggagttgtta catttttaga ggagaaagaa tatgcttcag gatacaatta
     1501 ttttgagaac ccatatgtca gtacaatgat tggtacaata gaaacagagc ttcacaaaaa
     1561 cggatacttc accatgcttc aatcagtatc cagaagctca gatatattat ctttggttaa
     1621 gaactggaat gtagatggga tgattctggt tttccccacc tctgcccaaa atcttgaaaa
     1681 gctgatggat gcggccaact gccctattgc aacctttgac agcaattact ctcaccctaa
     1741 tctcataaat gtaatatctg atgatgaaaa gggcttatat ttgtcaacca aatatatgat
     1801 aaatcacggg cacactgaga ttgcctttgt agctgattat gagggaaata tcgtcctcac
     1861 caaacgtttt aacggataca aaaaagcttt ggaggacagt catattcctt tccggcctga
     1921 atatattttc agctattcgc cttcatatga gggtggtgtc gaggccggcc gtgccattgc
     1981 aagcagcaag gcaccaatca cagctgtggt aacaactgcc gacatatgtg ctatcggcat
     2041 catggaggga gcgcgtctcg gtggttaccg tgtcccgatt gatttgtctg tcataggcta
     2101 tgataatttg aatctatgcc agtacaccgt accgaagctt acatcggtat cacaaaatgt
     2161 accaaaaaaa gcccggcttg caaccgagct tctcctaaag aaaatacatg acaatgaggc
     2221 agactgtaat ttcggtgaga ttatggatgt tgagattgtt gacagacaat cagttatctc
     2281 gctttactaa tccctgcgca cacgcttgtt ttcatacatt ttctcatcag cctcacagat
     2341 aatcttattg atatcggcgt atctgccgca ggcatagcct actgatgcgg aaatcggaat
     2401 tttgctgtcc ttttggattg gttccataat cttattccat ctttcaataa agctaagtgc
     2461 atcatcctca gtcccatccg gtatgatcat gataaactca tcgcctccat agcggtatac
     2521 tcttacatta tcattatcat ttgctgcagc cagtctgatg gttcccgcta ttcttgctat
     2581 aagacggtct cccttcccat gtccgtatgt atcgttcaca aacttcagct gatttacatc
     2641 ccaatatatg acagctacct ttcggtcagg ctcctttggc ttttcaaggt catcaaggag
     2701 cttattttta ttgtaaaggc cggtcatgtc atccattgca gcctctttct tgatacggtg
     2761 cgctttttcc attgttcttc gaagcatttt attaacacca ttgctgacac tgtaaaatcc
     2821 ggctgctata agtgaacgag gaaaaacata atacataaaa agagtaaacc cgggattttt
     2881 attcacctca ttcagcaaaa atactccatc cttttgaaga tgctcaagtg atgtcgcagt
     2941 aagcaatacc atatttgcat cgcacactcc aaaataatag cccacatatg tactaagcgc
     3001 tatattaaaa atcgaaaaag ctaatgcaaa tctcgacagg tgctttgatg aatatacacc
     3061 tgcaattaca atcggtaaca tcacagctat tatcatatga tatgtaagcg acgagcacgt
     3121 tatcatcact agaaaacctg cctctgaaat gcacagatac tttgcacagg cttgctccag
     3181 tccaactatc atcagcagca caataaacac cagcagaaga atacaggata atatatatcc
     3241 tttaacaaaa atattattat caacaataaa tatattaagg cagttaagta tgaatacaaa
     3301 tgtatatagc aaatgccata tcaaaagaat acgaaacaca aatatatttt caacaacatc
     3361 cttcttctca tatataggat tatcatctgt aatttttgaa aacaacagct ttaacgtctg
     3421 cattataatt ccccttttcc ttttctcttt cccttatctg gtcaaatcct ttacccactt
     3481 gtatttctta aaatgtatga gtgtccatgg tatctttcca atctcatcca cgcaggtgca
     3541 cgcatacacc acaaccaccg gccagttcag gaaatatgta cctgcaactg caagcggtat
     3601 cgctatgccc cacatcgtaa cagcaagaga atacatatca aacatcgtat ctcctcccga
     3661 accaaagaca ccgtttatga tgatttcatt taccgagcgt ccgatcatgt agaaggcaag
     3721 tacaccaaac agctgcttca gataccctgc tgcctgcggt gtgagcttta caaaatgtaa
     3781 aatcaccggt gctgaaaccg ccataagcac tgtcataaca gcgccacaca aaaatgacag
     3841 ttttgccatt ctgatgccat atgtttttcc acgctttaaa tttcctgcgc ccagctcatt
     3901 tcctatcatg atgccggcag cgcttgatat acctgcactg agacagcaaa tgatatcacg
     3961 cacaaccgct gcgactgagt tggctgctgc cgcgtctgtt cccatgtgtc ccataaatgt
     4021 agagtacgat gtaaagccta tgccccatac aaggcttgct ccaagaatcg gcccggagca
     4081 cttcataaaa tctgctgaca gctctctgtt ccttgcaaaa agctctctaa ttttaagcct
     4141 gatgtaaccc ggtctgtacg aaaatgtgat tgaaagcatg agctccacta ctcttgatat
     4201 aagcgttgca agtgccgctc ctctcacatt catgctctcg atgccaaatg ccccatagat
     4261 aaataccgca ttcagcacga tatttataag tactgtaata gagctgaccg cagccgcagt
     4321 acccgcgtgc tctgaaatct tcatcatgac tatataacat tgtgaaaaac ctgtcaataa
     4381 atatgaaaaa gaagctattt tcagatagct tacaccataa tctataagca ccggctcgtt
     4441 tgtgaaaata agcatcagat atcgtggaaa aaatgcacat gccacgaaaa acagcaccga
     4501 tactatgcca catatcctga ttgcaagagc gaatatgtca tcgagcgcct ttatgtcctg
     4561 cttgccccaa tactgcgctc cgagtattgc aagccctgct gtcgctgctg atagaaacat
     4621 gttctggatg aactgtatct gtgttgcgag cgaaaccgct gacatatagt tctggtccag
     4681 tcctccaagc atgaatgcat ctgccaccgc caccagcgcc agcatcagat tctgcaccat
     4741 tatcggaacc atgagtgtcc acagcttttt gtaaaaatgt gtatcaaatt ctattgtttt
     4801 cataagtaaa ttttacctct atatataaat ctctaaaggt ttacaagtat ccgtgtccat
     4861 gcttcgcata gcctttcaaa tgtccatgcc gcatttgcag ggct
//
LOCUS       DPUF01000220            9253 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6420, whole
            genome shotgun sequence.
ACCESSION   DPUF01000220 DPUF01000000
VERSION     DPUF01000220.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6420"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="DHV81_07765"
     CDS             complement(<1..92)
                     /locus_tag="DHV81_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate--pyruvate lyase"
                     /protein_id="HCI94344.1"
                     /translation="METWYYDVVSIDGDYANLKRCDIESDDLKLV"
     gene            complement(111..506)
                     /locus_tag="DHV81_07770"
     CDS             complement(111..506)
                     /locus_tag="DHV81_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCI94345.1"
                     /translation="MFFMMGITDGRKDFDFNQVITCDKCGRYGRYQVYMTYMVLSLFF
                     IPCFKWNRHYYVQTTCCNALYELDPEIGRRIARGEDVEILPQHLQRVNPQYGYGFENG
                     SGNTIKRCSNCGYTTTEDFEFCPKCGRRL"
     gene            complement(522..845)
                     /locus_tag="DHV81_07775"
     CDS             complement(522..845)
                     /locus_tag="DHV81_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCI94346.1"
                     /translation="MSDYDSKIIRKQIRVYGSVQGVGFRYRTEHAAESVGATGWVRND
                     PDGSVFMEIQGTEEQIDRVFAMVSQGTYVNIEKMDAKSIPVVEDEWGFHTRESLGDFW
                     GLWHR"
     gene            complement(882..2441)
                     /gene="cls"
                     /locus_tag="DHV81_07780"
     CDS             complement(882..2441)
                     /gene="cls"
                     /locus_tag="DHV81_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCI94347.1"
                     /translation="MKEKTYEGTKKTQNGLKRMFFSALAILLQAVFMVYMFTRLNEYA
                     AAINTTTSVLAIILVLGLYNREQTASMTTPWIILILAFPIMGVSLYLLVGLNGAPNKM
                     RARFEEVDSMLLPCLPENADILSDMYEKVPQAAGISTYLAKNALYPVYQNTDVTYYDQ
                     ASKGLEAQLSDLSKAKKFIFMEYHAIEDKESWHRIQKVLTERVQAGVEVRVFYDDMGS
                     IFFIDRDFSEKMEKLGIKCRVFNPVAPAFNMFLNNRDHRKITVIDGKIAYTGGYNLAN
                     EYFNVTHPYGIWKDTGIRLEGDAVKSFTIAFLEMWNASERKVPREVDVSQYLLHYDYE
                     AKQSGFIQPYADSPLDNEQVGEDVYISMANKAQRYCWFITPYLIITDEMSHALTLAAK
                     RGVDVRIITPGIPDKKPIYSVTRSFYNQLVKHGVRIFEWTPGFCHAKMSVADDIMATC
                     GTINLDYRSLYHHFENGCFMADCEVVHEIRDDFIRLFAESAEVTEKYRTGRSARRRLG
                     QSIMRLFAGLL"
     gene            complement(2508..3374)
                     /locus_tag="DHV81_07785"
     CDS             complement(2508..3374)
                     /locus_tag="DHV81_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCI94348.1"
                     /translation="MSDIKLGCHVGMAGKDMFLASAREAASYGANVFMLYTGAPQNTR
                     RKEIFELNIDAGWEYAHEHGINEIVVHAPYIINLANTVKPETYELAVEFLEKEIVRTA
                     AMKSRILVLHPGSHVNAGEQAGIAQIVKGLNTVLNQNDDDVFIALETMAGKGSEIGRS
                     FEELKAIYDGVDRKERLRVCFDTCHVNDAGYDIVNHYDEVFAEFDRVIGLEQIAVFHV
                     NDSLNPLGAHKDRHANIGKGTIGYDTLHRLVHDETFANVPKILETPWLCEEGSAKKTI
                     PPYKEEIEWLLK"
     assembly_gap    3542..3614
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3641..3928
                     /locus_tag="DHV81_07790"
                     /pseudo
     CDS             3641..3928
                     /locus_tag="DHV81_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
     assembly_gap    4063..4214
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4215..4961
                     /locus_tag="DHV81_07795"
     CDS             <4215..4961
                     /locus_tag="DHV81_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCI94349.1"
                     /translation="KGKEYYDAIREGAEVKTGLISSGIFEDTFREIIEADKDVLYFSL
                     SKNISGNYNSARVAADAVLDDCSNGRKIRLVDSLNASLGQGLLAIYASEMREKGMSFD
                     EVADTIETYPARLNGVFTVGNLKYIARTGRLSGTTALVGNMLSIKPILRGSKDGYIVQ
                     FRKCRGRKAVLNELVNLLCDNITDPENQIIGVAHADAYEDSLYVIEQIKKRIKVRDVI
                     NTTYDFCTGSHVGPESLAVFFMGKDRELGA"
     gene            complement(5081..6793)
                     /locus_tag="DHV81_07800"
     CDS             complement(5081..6793)
                     /locus_tag="DHV81_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HCI94350.1"
                     /translation="MSGILMMVIAIVVLGGAYLLYGRYLQNKWGIDPNAKTPAYEMED
                     GVDYVPADTNVVFGHQFASIAGAGPINGPIQAAIFGWLPVLLWILIGGVFFGAVQDFA
                     SMYASVKNKGRTIGYIIEEYIGKLGKKLFLLFCWLFCILVVAAFADVVAGTFNGFAAD
                     KAGEVTKVVANGAVATTSMLFIIEAVALGFFLKYSKFNKWINTLVAIVLLIVAIALGL
                     NFPMYLNLSVWHIIIFAYILIASVTPVWALLQPRDYLNSYLLIFMIVGAIIGVFVANP
                     ACNLDAFTAFAIPDANGNPQYLFPILFVTIACGAVSGFHSLVSSGTASKQIKNEKNML
                     PVSFGAMLMESMLAILALIAVASFGKGEAAAQGLTTQPQIFAGAIANFLSVIGLPHSL
                     VFTLINLAVSAFALTSLDSVARVGRLSFQEFWLDSDTDDDNMSPFVKLMTNKYFATII
                     TLVLAFLLTKVGYAEIWPLFGSANQLLSVLALVACAVFLKKTKRQGCMLWIPMVFMMA
                     VTFTALGMTIYKLTKALFSVGLDLGNSLQLIFAVLLLILGVLVAIQGVKKLCEKSESK
                     KAAA"
     gene            complement(6951..7979)
                     /locus_tag="DHV81_07805"
     CDS             complement(6951..7979)
                     /locus_tag="DHV81_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCI94351.1"
                     /translation="MKALQKVSKFLSDYTSIVVIAIAVVTFLVPNLMAWVNQMLFVDP
                     VSNKFTCQSIIIGIIMFSMGLTLTTQDFKILAQRPFDICVGAIAQYLIMPFLAFGISK
                     ALNLPDGIALGLILVGCCPGGVSSNIMSYLCGGDVAFSVGMTTVSTLLSPFMTPLLVS
                     FLASGTHVSIKALPMFVSIIETVIFPVAVGFLLNYLLGKKKAFAEAQKVMPGIAVLGL
                     AFVVGGVVSSQGSKFFTSGVVIFAAVLLHNGLGYLLGYGAGKLVGMNTAKKRTISIEV
                     GMQNAGLATNLATTTAQFASTPESAIICAVSCTWHSISGTLLAGAFALKDKMAAKKAE
                     EAAETKTA"
     gene            complement(8427..>9253)
                     /locus_tag="DHV81_07810"
     CDS             complement(8427..>9253)
                     /locus_tag="DHV81_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94352.1"
                     /translation="ASWIMYAKAGEHGWAAIVPFYSSYVKFRIAGKQKLFWGYLVASI
                     ASIAGCILLMYEIIASGLSVMTSSYMGAYYDSTYGYAGNRIGAHMGMLIFAVILIIAA
                     MIAALVMNILCCVGLAHAFGKGAGFACGLIFLNVIFICIIAFNKNIVYVGAGYNSNNN
                     YYNPYGSNGYGQQYGQNMYGNGANQQYGQNMYGNGANQQYGQNMYGNGANQQYGQNMY
                     GNGTNQQYGQNMYGNGANQQYGQNPYGTAQGGDAQNSYGTSQQQSWNDEYNNKDYE"
BASE COUNT         2482 a         2144 c         1741 g         2659 t
ORIGIN      
        1 acaagcttca gatcgtctga ttcgatatcg catctcttta gatttgcata gtctccgtct
       61 atggacacta catcgtaata ccaggtttcc atgtgttttc ctcctaattt ttacagcctc
      121 ctgccacatt ttgggcagaa ttcaaagtcc tctgtagttg tatatccaca attgctgcat
      181 ctttttattg tgtttccact tccattttca aaaccgtaac catactgcgg gtttacacgc
      241 tgcaaatgct gtggcaatat ctccacgtcc tcgccccttg ctatcctgcg tcctatttcc
      301 ggatcaagct cgtaaagcgc attacagcat gtggtctgca catagtagtg cctgttccat
      361 ttaaaacacg gaatgaagaa cagtgacagc accatgtatg tcatatacac ctgatacctg
      421 ccatacctgc cacatttatc gcaggtaatg acctgattga agtcaaagtc cttcctgccg
      481 tccgttattc ccatcataaa aaacatatat gatgcctccg tttatctgtg ccatagtccc
      541 cagaagtctc caagcgattc tctcgtatga aatccccact catcctcaac caccggtatt
      601 gatttggcat ccattttctc tatatttaca taagtaccct gactcaccat tgcaaatact
      661 ctgtctatct gctcctctgt gccctgtatt tccataaata ccgagccgtc agggtcattt
      721 ctcacccagc ctgttgcacc aacgctttca gccgcgtgct ctgttctgta gcggaaacct
      781 actccctgca cagagccgta cactcttatc tgctttctga tgattttgct gtcgtaatca
      841 ctcatatgct ctcacttcct aaataaaact tagttttccg tctacaacag tcctgcaaac
      901 agccgcataa ttgactgccc caaccttcgt cttgcacttc ttccggtcct gtacttctcc
      961 gtcacctccg ctgactcagc gaaaagcctg ataaaatcat ccctgatttc atgcaccact
     1021 tcacaatccg ccataaaaca gccattttca aaatggtgat aaaggcttct gtagtcaaga
     1081 tttatggtac cgcaggttgc cattatatcg tctgccacac tcatctttgc atggcaaaag
     1141 cccggtgtcc actcaaaaat cctcacacca tgcttcacaa gctggttgta gaaggagcgt
     1201 gtcacgctgt atattggttt cttatcaggg atgcccggcg tgatgatgcg cacgtcaact
     1261 ccacgctttg cggcaagtgt cagcgcatgg ctcatctcat cggtgatgat aaggtaagga
     1321 gtgataaacc agcaatagcg ctgtgccttg tttgccatgc tgatatagac atcctcaccc
     1381 acctgctcgt tatcgagagg gctgtctgca tatggctgga taaatccact ctgttttgcc
     1441 tcataatcat agtgcagcag atattgtgat acatccacct ctcgcggcac cttgcgctct
     1501 gatgcattcc acatctcgag aaatgcgatg gtaaacgact tgacagcatc tccctcaagc
     1561 ctgattcccg tgtccttcca gattccgtat gggtgcgtca cgttgaagta ctcatttgcc
     1621 agattatagc cgccggtata tgcgattttt ccatcaatta ccgtgatttt tctatggtca
     1681 cgattgttta aaaacatgtt aaaggcaggt gcaaccgggt tgaacaccct gcatttgatg
     1741 ccaagcttct ccatcttttc cgagaaatcc ctgtctatga aaaagattga acccatatca
     1801 tcataaaaca cccttacctc gactcctgcc tgcacacgct ccgtgagcac cttctggata
     1861 cggtgccacg attccttgtc ctctatggcg tgatactcca tgaaaataaa tttctttgcc
     1921 tttgataaat cgctaagctg cgcttcgagt ccctttgacg cctgatcata atatgtcaca
     1981 tccgtgttct gatataccgg atataatgca ttttttgcca ggtatgttga aatccccgct
     2041 gcctgtggca ccttttcata catatcagat agtatgtctg cattttccgg caggcatgga
     2101 agaagcatgg aatccacctc ttcaaaccgt gcacgcatct tattcggtgc gccgtttagt
     2161 ccgactaaca ggtaaagtga cactcccatt atcggaaatg ccagtatcag tataatccat
     2221 ggtgtagtca tggatgcggt ctgctcacga ttgtacagtc cgagcacaag tatgattgcc
     2281 aggacgcttg tggtcgtgtt tattgctgcc gcatattcat ttagccttgt aaacatatag
     2341 accatgaaaa ctgcctgcaa taatatcgca agcgctgaaa aaaacattct ctttaaacca
     2401 ttctgtgttt ttttagttcc ctcgtaggtt ttttccttca tctattatgc ctccattatc
     2461 ttactgtctg taataccaaa cctctccata catttttagc caccttctta tttaagaagc
     2521 cattcaattt cctctttata aggaggaata gtcttcttcg cagagccctc ctcacaaagc
     2581 catggtgttt ccagaatctt tggtacattt gcaaaggtct catcatgcac caggcggtgc
     2641 agtgtgtcat agccgatggt tccctttcct atatttgcat gcctgtcctt gtgtgctccg
     2701 agaggattta agctgtcatt cacatgaaat accgctatct gctcaagccc tatcaccctg
     2761 tcaaactccg caaaaacctc atcataatgg ttcacaatat cgtatcctgc atcatttaca
     2821 tggcacgtat caaagcagac acgcagcctt tctttcctgt ccactccatc atagattgcc
     2881 ttaagctcct caaaggaacg gcctatttca ctgccctttc ctgccatcgt ctcaagtgca
     2941 ataaacacat cgtcatcatt ctgattgagc actgtgttaa gtccctttac aatctgtgcg
     3001 attccagcct gctcaccggc atttacatga cttcccggat gaagcaccag tatgcggctc
     3061 ttcattgccg cggtccttac tatctccttt tccagaaact ccactgccag ctcgtatgtc
     3121 tcaggcttta cggtgtttgc cagatttatg atgtatggcg cgtggaccac tatctcattt
     3181 atcccatgct catgtgcata ttcccatcct gcatctatgt tgagctcaaa gatttccttt
     3241 cttctcgtgt tctgtggtgc cccggtgtag agcataaaca cattcgcgcc gtatgacgct
     3301 gcttccctgg ctgatgccag aaacatatct tttcctgcca tgcctacgtg acagcctaat
     3361 tttatatcac tcatattttt cctctgtttc attgaatcta tatatctatt atttctacta
     3421 taacactaca tccctgataa ctctatctaa atattctaaa tttttactaa acatggcaaa
     3481 agtcattgac atagtatcaa aaactacata tactatcctt tgcaaccaca tatattaatg
     3541 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnattatt atagaaatac gagggtaaat atgtttgaaa taattgttga
     3661 ctctgcggca aacattcctg cagagctatg taaaaaatat aaaatcaagg tgctatcatt
     3721 tgtaaacctt gtcaacggaa aggaaatgac ctgctttgag cctgacctca caccggaaca
     3781 ggagcgggca aagggaaagg aatactatga tgctataaga gaaggtgccg aggtaaagac
     3841 cggacttatc agcagcggaa tctttgagga taccttcagg gaaatcattg aggcagacaa
     3901 ggatatcctg tatttctccc tcagcaaann tttgcaacca catatattaa tggcatataa
     3961 ttgtaatgta tgtgatgcat aactacagtt aaccaattat tatgattaaa ggggcagaat
     4021 accttttgac catcatatca ttattataga aatacgaggg tannnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnaagggt aaggaatact atgatgcgat aagagaaggt gctgaggtaa
     4261 agaccggact catcagcagc ggaatctttg aggatacctt cagggaaatc atagaggccg
     4321 acaaggatgt cctgtatttc tccctcagca aaaacataag cggcaactac aactcagccc
     4381 gtgtcgcagc cgatgctgtc cttgatgatt gcagtaacgg cagaaaaata cgcctggtgg
     4441 attcattaaa tgcttccctc ggacagggac ttctcgcaat atatgccagc gaaatgcgtg
     4501 agaagggcat gagctttgat gaggtggctg acacgataga gacctatcct gccagattaa
     4561 acggtgtgtt taccgtgggc aatctaaagt atattgcaag aaccggcaga ttaagcggaa
     4621 ccaccgcttt ggttggcaac atgctaagta tcaagcctat cctaaggggc agcaaagacg
     4681 gatatattgt acagttcaga aaatgtcgtg gccgaaaagc cgtattaaat gagcttgtca
     4741 atctgttatg cgacaacatt acagaccctg aaaatcagat tatcggagtg gcacatgctg
     4801 acgcatatga agattcgctc tatgtgattg aacagattaa aaagcgcata aaggtgcgcg
     4861 atgtgataaa taccacctat gatttctgca ccggaagcca tgttggtccg gaatctttag
     4921 cagtattttt catgggaaag gacagagaat tgggagcctg atatcaggct cccttttcac
     4981 ttcttttatt catcatcctc acataaaaaa gtcccagaac tcgcttatta agcggattct
     5041 gagacttttg atttctatta attgcaactt ctatctcatt ttaagctgca gctttcttgc
     5101 tttctgattt ctcacagagc ttcttcacac cctgaattgc aacaagcaca ccaaggatga
     5161 gcagtaatac tgcgaatata agctgtaatg aatttccaag gtcaagtcct acagagaata
     5221 atgcctttgt aagcttgtag attgtcatgc caagtgcagt aaatgtaact gccatcatga
     5281 ataccattgg aatccagagc atgcagccct gacgctttgt cttctttaag aatactgcgc
     5341 aggcaacaag tgcaagtact gataagagct ggttagctga accaaacagt ggccatatct
     5401 ctgcatagcc tacctttgta agcaggaatg caagtaccag tgtgatgatt gttgcaaagt
     5461 atttgtttgt catcagcttg acaaatggac tcatattgtc atcatctgtg tctgaatcaa
     5521 gccagaactc ctggaatgac agacgtccga ctctggctac tgaatcaagt gatgtcagag
     5581 cgaaggctga tacagcaagg ttaatcagtg tgaacacaag tgagtgtggt aaaccgatta
     5641 ctgacaggaa gtttgcgata gctcctgcga aaatctgtgg ctgtgttgta agtccctgag
     5701 ctgctgcctc tcccttaccg aatgatgcta ctgcgataag tgcaaggatt gcaagcatac
     5761 tctccataag catggcgcca aatgaaacag gaagcatgtt cttctcattc tttatctgct
     5821 tagaagcagt acctgatgat acaagtgaat ggaaacctga aactgcacca caggcgattg
     5881 ttacgaataa gattgggaac agatactgtg ggtttccgtt tgcatcaggg attgcaaatg
     5941 ctgtgaaagc atcaaggtta caagcaggat ttgcaacaaa tacaccgatt atcgcaccta
     6001 cgatcatgaa gattaacagg tagctgttaa ggtaatcacg tggctgaagc agtgcccata
     6061 caggtgtaac actggctata agtatatatg caaatataat tatgtgccat acgctaaggt
     6121 ttaaatacat tgggaaattg agtcccagtg ctatagctac tataagtaat acgattgcaa
     6181 caagtgtgtt aatccatttg ttgaacttag agtatttaag gaagaaacca agtgcaactg
     6241 cttctatgat gaaaagcatt gatgtagttg caactgctcc gttggctaca acctttgtaa
     6301 cctcgcctgc tttatcagca gcaaagccat tgaaggttcc ggctacgaca tctgcgaaag
     6361 ctgcaacaac aagtatacag aacagccagc agaataacag gaataatttc tttcctaatt
     6421 taccgatgta ctcctcgatg atgtaaccga ttgtacgacc cttgttctta actgaagcgt
     6481 acatagatgc aaagtcctga acagctccaa agaatacacc accaattaat atccaaagca
     6541 gaaccggaag ccatccgaaa atagctgcct gaattggtcc gttgattggg cctgcacctg
     6601 ctatagatgc aaactgatgt ccgaatacta cattggtatc ggctggtaca tagtccactc
     6661 cgtcctccat ctcgtaggca ggagtcttgg catttggatc aatgccccat ttgttttgca
     6721 gataacggcc atataataag tatgctccac cgagcacaac aatggctatt accatcatta
     6781 agattccact catattatta cctctttctt ttgtttatct taattttatt tatatattat
     6841 gtttactctt tttttagcaa atttcaataa atattttata cataatttat tttttatttc
     6901 tataattttt gtttaattta ccaatcggtt ataataggta ttcattctct ttatgctgtc
     6961 tttgtctcag cggcttcttc tgccttttta gcagccattt tgtcctttaa tgcaaaggct
     7021 ccggcaagca gtgtgcctga gattgaatgc catgtacatg acactgcaca gatgatggct
     7081 gattcaggtg ttgaagcaaa ctgtgctgta gtggtagcaa ggtttgtggc aagacctgca
     7141 ttctgcatac caacctcgat agagatggtt ctcttcttgg ctgtgttcat gcctaccagc
     7201 tttcctgcac cgtatccaag cagatatcca agtccgttat gtaacagaac tgccgcaaaa
     7261 attactactc ctgatgtaaa gaactttgag ccctgtgatg atacaacacc acctacaacg
     7321 aatgccaggc caagcaccgc aatacccggc ataaccttct gagcctcggc aaatgccttt
     7381 ttctttccaa gcaggtagtt gagtaaaaat ccaaccgcta caggaaagat tacagtctca
     7441 atgattgaca caaacatcgg aagcgccttg attgagacat gtgttccgct tgcaagaaat
     7501 gataccagaa gcggtgtcat aaatggtgag agcaatgtgg aaactgttgt cattccaact
     7561 gagaatgcca catcaccacc gcagaggtat gacataatgt ttgaagaaac accaccggga
     7621 cagcagccta cgaggataag tccaagtgcg attccgtcag gcagattcaa tgcctttgag
     7681 ataccaaatg cgagaaacgg catgataaga tactgtgcga ttgcacctac acatatatca
     7741 aatggtctct gtgcaagtat cttgaaatcc tgtgtcgtaa gtgtaagtcc catactaaac
     7801 atgatgattc cgataatgat tgactgacat gtgaacttgt tggataccgg atccacaaac
     7861 agcatctgat ttacccaggc catcagattc ggaaccagaa atgtgactac ggcgattgct
     7921 atgacaacta tcgatgtgta gtctgataaa aatttactta ctttttgtaa tgctttcatt
     7981 gtaagcctcc ttaacatttt tctgctcact aaaactcttt tatggaatta aaaaatccct
     8041 ttccagattg acataatctg aaaagggacg aaataatccg cgttaccacc ctgattccac
     8101 agtgtattac tgtgacactc tgcataccag catatgcttt ccatataacg gtggacaccg
     8161 gcgaggttta cagacacact taatgctttc agcccgcttc ttagagagga tattccaatc
     8221 tgatcattgc ctgttcgcac cgcccacagg ctctctgaaa ataaatacaa atcggacctt
     8281 gttctcatca acgaattttg gtgaacatat ttatttaaat cttgttttgt attatagccc
     8341 tctgacacta tatgtcaaga gaatgataca aatttgtact ataacattta tttatagttt
     8401 ttattcatgt gctttattct tattatttat tcgtaatctt tgttgttata ctcatcattc
     8461 catgactgct gttgtgatgt accgtaggaa ttttgagcat ccccgccctg tgctgtgcca
     8521 tacggattct gaccatactg ctggtttgct ccgtttccgt acatgttctg gccgtactgc
     8581 tgatttgtgc catttccgta catgttctgg ccatactgct gatttgcccc gtttccgtac
     8641 atgttctggc cgtactgctg gtttgctccg tttccgtaca tgttctgacc gtactgctgg
     8701 tttgctccgt ttccgtacat gttctgacca tactgctgac catatccgtt tgaaccgtat
     8761 ggattatagt agttattatt actattgtat ccagctccca cataaacaat gtttttgttg
     8821 aaggcaataa tacagataaa aatcacattc aggaatataa gtccgcaggc aaatcctgct
     8881 cccttgccaa acgcatgtgc cagtccgaca cagcagagga tattcataac caatgccgca
     8941 atcattgctg caataatcag aataaccgca aatatcagca tgcccatgtg agccccgatg
     9001 cggtttccgg catatccata ggtagaatca taatacgccc ccatataact ggatgtcata
     9061 acggaaagtc ctgaggcaat aatctcatac atcaataaaa tacatcctgc tatacttgcg
     9121 atacttgcca caagatatcc ccagaacagc ttctgtttcc ctgcaatcct gaattttaca
     9181 tatgagctgt aaaacggtac aatggccgcc cagccgtgct ctcctgcctt tgcatacata
     9241 atccatgatg caa
//
LOCUS       DPUF01000221            4879 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_25845, whole
            genome shotgun sequence.
ACCESSION   DPUF01000221 DPUF01000000
VERSION     DPUF01000221.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_25845"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="DHV81_07815"
     CDS             complement(<1..1064)
                     /locus_tag="DHV81_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HCI94353.1"
                     /translation="MQEYSVKVTLPDGQVMAVTASESDTLEAVADRFKDYYEDDIILG
                     IVNGRLRELNKKIKSDCELSFVTTADRDGRRTYRRSVVLLLQRAIYDVYGSMTQLHVM
                     HSLGEGYYCQLEKAVECADSQQEKYNEDTDLQGSRENSEKSVTEHDIDRIVCSMYSFV
                     EKDLPITKHSAKTQYAEQLFKEKGLHDKERLLHYRRSSRVNLYELDGVVDYFYGFMAP
                     STGMLKYFDIVPYESGFVLLFPGAHSRSVEPLVTSNKLFHTLDDSREWSKMLGIGTIG
                     SLNDAIAAGRGQEIMLLQEALMEQKIGNLAAQIASDDKKKFIMIAGPSSSGKTSFANR
                     LSIQLIAKGRKPHPLSLDDY"
     gene            complement(1051..1773)
                     /locus_tag="DHV81_07820"
     CDS             complement(1051..1773)
                     /locus_tag="DHV81_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HCI94354.1"
                     /translation="MKAVASMVTAGYTLCDVGTDHGYVPIALVQGNIIPKAIAVDINK
                     GPLERANEHIRANGLTEKITTRLSNGLEAIHDGEVDSIVIAGMGGELVIHILTAGETV
                     CRSAKELILQPQSEVSKVREYVRNTGYKIVDEDMILEDGKYYPMFRCVPCADNSAWDN
                     MDETTVTVCDLYGPVLIKNGNPVLRKFLVREHHKLAAIMQQLRTQEMSDSIMDRIEQI
                     NEMMAYNEAAYSTMGAIRNAGI"
     gene            complement(1796..>2242)
                     /locus_tag="DHV81_07825"
     CDS             complement(1796..>2242)
                     /locus_tag="DHV81_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HCI94355.1"
                     /translation="RVSRQLLQELGREPSPEEIAKEMNMPVERVREILKISQEPVSLE
                     TPIGEEEDSHLGDFIKDDNVPVPADAAAFTLLKEQLEEVLGTLTEREQKVLTLRFGLE
                     DGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSRKLKDYLE"
     assembly_gap    2243..2405
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2406..3058)
                     /locus_tag="DHV81_07830"
     CDS             complement(<2406..3058)
                     /locus_tag="DHV81_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HCI94356.1"
                     /translation="MAKKKEQKDMAQEQEQRLNFQQKLIEILELGKKKKNMLEYQEIA
                     DFFKDLNLDPEKFEMVIDYLEQNGIDVLKISNDDDVDDDIILDDEDEVEVEKIDLSVP
                     EGVSVEDPVRMYLKEIGKVPLLSADEEIELAQNMEDGAVATEKINVLKGRLDGASEEE
                     KAEIKQEIKTLQRDVDKGADAKKRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGL
                     "
     gene            complement(3072..4858)
                     /locus_tag="DHV81_07835"
                     /pseudo
     CDS             complement(3072..4858)
                     /locus_tag="DHV81_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743186.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     assembly_gap    3741..3815
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1025 a         1302 c          871 g         1443 t
ORIGIN      
        1 tagtagtcat ccagacttaa cggatgtggc tttctgccct ttgcgataag ctggattgac
       61 agtctgttgg caaacgatgt cttgcctgat gatgaaggcc ctgctatcat gataaatttc
      121 tttttgtcgt ccgaagcaat ctgcgctgca agatttccta tcttctgctc catcagtgcc
      181 tcctgaagca gcatgatttc ctgtcccctg cctgctgcaa tcgcatcatt tagtgagcct
      241 atcgtgccta ttccaagcat cttgctccat tccctggaat catccagcgt gtgaaacagc
      301 ttatttgatg tcacaagcgg ctcaacgctc ctgctgtgag cacccggaaa aagaagcaca
      361 aagccactct catacggcac tatatcaaag tatttgagca tgcctgtcga gggtgccata
      421 aatccgtaga aataatccac caccccatcc agctcataaa gattgactct ggagcttctc
      481 ctgtagtgca ataacctctc cttatcatgc agcccttttt ccttgaaaag ctgctccgca
      541 tactgtgttt ttgcactgtg cttggtaatt gggaggtctt tttccacaaa tgagtacatg
      601 ctgcatacta tcctgtcgat atcatgctct gtcacgcttt tctcactgtt ttctctgctg
      661 ccttgtaagt ctgtatcttc attatatttt tcctgttggc tgtctgcaca ctccactgct
      721 ttctcaagct gacaataata gccttctccg agcgaatgca tcacatgaag ctgtgtcatg
      781 ctgccatata catcgtatat agccctctgc aaaagcagca caacacttct tctgtatgta
      841 cgcctgccat ccctgtcagc tgtcgtcaca aagctaagct cacagtcact ttttattttc
      901 ttattcagtt cacgaagcct gccgtttaca attccaagga ttatgtcatc ctcataataa
      961 tccttgaatc tgtccgctac ggcttctaaa gtatcacttt ctgatgctgt cactgccatg
     1021 acctgtccat ccggcaaggt caccttcaca ctatattcct gcatttctta ttgcccccat
     1081 agtcgaatat gctgcctcat tgtacgccat catctcgttt atctgctcga ttctgtccat
     1141 gatggaatca ctcatctcct gtgtccttag ctgctgcatg atggcagcca gcttgtggtg
     1201 ttccctgaca aggaacttcc gaagcaccgg attaccgttt ttgataagta caggtccgta
     1261 caaatcgcat acagtaaccg tcgtctcgtc catattgtcc catgcggaat tgtccgcaca
     1321 cggcacacat ctgaacatcg gatagtattt tccgtcctcc aaaatcatat cctcatcaac
     1381 tatcttatag cctgtgttcc ttacatattc acggactttg ctcacttccg actgtggctg
     1441 cagaatcagc tcctttgccg acctgcacac tgtctcccct gcggttaaaa tgtgaataac
     1501 aagctctcct cccattcctg ctatgacaat agagtcaacc tctccgtcgt gtatcgcttc
     1561 aagcccattg gaaagccttg tggtaatctt ctctgtcaga ccgtttgccc ttatatgctc
     1621 attggcgcgc tcaagcggtc ccttatttat atcaacagca attgccttcg gtattatatt
     1681 accctgcacc agggcaatag gcacatagcc atggtcggtt cccacatcac acagcgtgta
     1741 gccggccgtg accattgatg ccactgcctt cattcttctg gatagcctaa tcatcttact
     1801 ctaagtagtc cttaagcttt ctacttctgc ttggatgacg aagctttctt agcgcctttg
     1861 cctcaatctg gcggatacgc tcacgagtga cgttgaactc cttacctacc tcttcaaggg
     1921 ttctggctct gccgtcctct aagccaaagc ggagtgtgag taccttctgc tctctctctg
     1981 taagagtgcc aagcacctcc tcaagctgct ccttaagaag tgtgaatgct gccgcatcag
     2041 caggaaccgg tacattatca tccttgatga aatcaccaag atgtgaatcc tcctcctcac
     2101 cgattggagt ctcaagtgaa acaggctcct gtgatatctt gagtatctca cgcacacgct
     2161 ctaccggcat gttcatctcc ttggcaattt cctccggtga aggctctctg ccaagctcct
     2221 gaaggagctg tcttgacaca cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnagacc taagtttccc tcctgaatca ggtcaaggaa aagcattcct cttcccacat
     2461 atctctttgc gatgctgaca acaagacgca ggtttgcttc tgcaagacgc tttttggcat
     2521 ctgctccctt gtccacatcc ctctgcagag tctttatttc ttgctttatc tcagcctttt
     2581 cctcttccga tgcaccatca aggcggccct ttagtacatt aatcttctcg gttgctaccg
     2641 ctccgtcctc catattctgg gcgagctcaa tctcctcatc tgctgagaga agaggtacct
     2701 tacctatttc cttaagatac atacggacag gatcctcgac gctgactccc tccggtactg
     2761 agaggtcaat cttttcaacc tctacctcat cctcatcatc gagtatgata tcatcatcca
     2821 cgtcatcatc attgctgatt ttaagcacgt ctataccgtt ttgctcgaga taatctatta
     2881 ccatctcaaa cttctccgga tccagattta agtccttgaa aaaatctgca atttcctgat
     2941 attcgagcat attctttttc ttttttccaa gctcaagaat ctcaataagc ttctgctgga
     3001 aattcagtct ctgctcctgc tcctgtgcca tgtctttttg ttcctttttc tttgccattt
     3061 tattttcctc tttattctgg tattttttgc aattcttcga gtgcacgctt atcctcaata
     3121 atcctcatca tgccctccat atctgtaggt gccagatgct ttgagcgata gtctatgctg
     3181 ttttgcttga tacggacaac ggtttccctg agtgcggttg ccttgtcatt tttgccctcc
     3241 acctgatgga ttgtggcatt gaaaagccct gccacctcgc gctgttcgtc ctcatcggta
     3301 aacatgctta ctatcctggc cggatttact gtatgcgtgg tctcgtactg tgaaaaaacc
     3361 tccttagcca ccttgtggta cagctcttcg gtaaagtcat ctgcactgat acgccccttt
     3421 atcctgtcaa aaagtgaatt atcctctatg agccatgtga ggagaagctt ctgcgactgc
     3481 ttcatgccat cctccttttt atttttctca ttgattccac ttttaagctt tgtctggggc
     3541 tttacgatac cgcccttcat tgccatactg tttacgagct ttcttaagtc gtcgtatggt
     3601 atgctgtact ctctggacac tgcctgcgta tagtttgtcc gctcaagctc ctcggtaaaa
     3661 ccaagcagct tctttgcgat ttcattataa aatgcggttt ttgactcggg atcgtccata
     3721 tcatagcctg cctgcatgat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccaag cgccttgata aactcatccg
     3841 ggtccttgta cggccgcatg ttaatgacct ttgttgtgat gcctacctcc ttgagtatcg
     3901 gaatcgcacg cagcgctgcc tttaccccgg cgccatcact gtcatatgtc agatacacct
     3961 cattggtgta tctctttaat agtgaggcat gtccgctcgt gagcgccgtg ccaagcgacg
     4021 ccaccgcatt gtcaaagccc gcctgatgca gtgctatgac atccatgtag ccctcacaca
     4081 aaaggagctg gtttttcctc gtgctccttg ctatattaag tccgtaaaga ttacggctct
     4141 tatcaaatat ctttgtctcc ggagagttta aatactttgg ctctccgtca cccatcactc
     4201 ggcctccaaa gcctatgaca cggttgtgca catccataat cggaaacatc gcacgattcc
     4261 agaacttatc gtgcccgcct ctgacctcat ctatcgtcac aagacctgtc tccttaagta
     4321 tctcatcatc atagcccttg ctgcgcagat acttgtacag atcatcactg tactggtccg
     4381 agtaaccaag cccgaacttc tgcattgtct catcggacag ctctctcttt ttgaaatact
     4441 catgcgccct ttgtcctctc ggtccacgaa gcagcatata aaaatacttt gcagcctctt
     4501 tatttatctc aagcagctgt gtacgcctgt ctgcctccct cctttgtgct gaggtcatct
     4561 cctgcttggg aagctcaatt cccacacggt ctgcaagctc ctgcatggcc tcagggaagg
     4621 taaagttctc atactgcatg agaaatgtaa atacattgcc tccggctcca cagccgaagc
     4681 aatagtacat ctgcttattc ggtgagactg aaaaggatcc tgtcttttcg ttatggaatg
     4741 ggcataatcc aaaataggta cttccctttt tctgaagatg cacgtagctt cctataacat
     4801 cgactatgtc atttcgcgaa cgtacctcct ctataagctc atcagagtag tacggcattt
     4861 tatcatcccc ttactgttc
//
LOCUS       DPUF01000222            6182 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_2963, whole
            genome shotgun sequence.
ACCESSION   DPUF01000222 DPUF01000000
VERSION     DPUF01000222.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_2963"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="DHV81_07840"
     CDS             complement(<1..221)
                     /locus_tag="DHV81_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCI94357.1"
                     /translation="MQNDTIAAIATAMSPSGIGIIRISGDDALSVIDRIYKSKNNKKK
                     ISACQSHTIHYGFIYDGDEKIDEVMVLLM"
     gene            complement(305..931)
                     /locus_tag="DHV81_07845"
     CDS             complement(305..931)
                     /locus_tag="DHV81_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HCI94358.1"
                     /translation="MEYIEVSAKTIDDALTEASIKLGTTSDKLEYEVIDKGSNGFLGI
                     GSRDAVIKVKKAEASVEDEIREFLDSVFKAMKLEVTIDIKVDEDNRNVDVELNGPEMG
                     VLIGKRGQTLDSLQYLTNLAINKQSENYYRVKIDTEDYRKRRRETLENLAKNISFKVK
                     RTKRPVSLEPMNPFERRVIHSALQNDRFVETHSEGDEPYRHVVVTLKK"
     gene            complement(944..2260)
                     /locus_tag="DHV81_07850"
     CDS             complement(944..2260)
                     /locus_tag="DHV81_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein J"
                     /protein_id="HCI94359.1"
                     /translation="MSDILLTAYPGSILGPIAKLLGILMDWIYSGISNITGGRVESVV
                     LSIVIITIIIYMCLLPLTIKQQKFSKLSQKMQPEMQAIQAKYKNKKDQASMMAMQEET
                     QLLYQKYGISPMGSCVQMLIQMPILFALYRVFYNIPAYLSGVKSSFTGLVDSIQQTSG
                     YQNTLVSLMEKYNVVTSSGLNASNAASKLADASGDTLSNYIIDILYKLPSKGWDALMD
                     GKFFDGIQSAVEKTHDALLHFNYFLGLNISDTPWYIIKSNFTDKPDKWLLFVILALLI
                     PVLSYLTQMLNIKLMPQATNGNDQMASQMKMMNLMMPFMSFVICFTVPVGLGIYWICS
                     ALVRGIQQFFVNRHIENLDLEAVMAKNEEKAKKKREKMGLSEDYIKKAAQIKTKSIDN
                     KANVSVSAGTEEKLAKAAEYKANAKAGSLASKANMVKEFNERNSRK"
     gene            complement(2278..2487)
                     /locus_tag="DHV81_07855"
     CDS             complement(2278..2487)
                     /locus_tag="DHV81_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCI94360.1"
                     /translation="MKSILIFLIKFYRKYISPMKSTKCPYCPTCSEYGLQAVQKYGAI
                     KGGLLAAWRILRCNPFSKGGYDPVP"
     gene            complement(2514..2861)
                     /locus_tag="DHV81_07860"
     CDS             complement(2514..2861)
                     /locus_tag="DHV81_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCI94361.1"
                     /translation="MKFSDSLKKNKDFQNVYNNGVSFANRYLVMYVLKNNTENNRIGI
                     SVSKKVGNSVIRHHITRLVRESYRLHEDMFDSGLDIVVIARVAARHVGYKEIESAVLH
                     LGGLHRIIKKQNI"
     gene            complement(2920..3054)
                     /locus_tag="DHV81_07865"
     CDS             complement(2920..3054)
                     /locus_tag="DHV81_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCI94362.1"
                     /translation="MKMTFQPKKRQRAKVHGFRQRMKTAGGRKVIAARRLKGRKKLSA
                     "
     assembly_gap    3353..3466
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3505..4866
                     /locus_tag="DHV81_07870"
     CDS             3505..4866
                     /locus_tag="DHV81_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCI94363.1"
                     /translation="MDKVMEKWNEILQMVKEEHGLTDVSFNTWIKPLEVFAIDGNTLY
                     ILVPSEQMGLSYINKKYYLPLKVAIGEVTGIEYDIQFILPDQAQSLKFKNNDTPQLEP
                     QTPVKGDHSNLNPNYTFDTFVVGNNNRFAHSASLAVAESPGEAYNPLYIYGGPGLGKT
                     HLMHSIGHFIIDQNPDTKVLYVTSEEFTNEVIESIRNGNSSAMTKFRDKYRTVDVLMI
                     DDIQFIIGKESTQEEFFHTFNTLQTQGKQIILTSDKPPKEMETLEERIRSRFEWGLMA
                     DIGTPDYETRMAILRKKAETDNFDIDDDILNYIASNISSNIRELEGALNKLLAFHNLE
                     HTHITMDIAERELSNIITPDKPREITAQLIIEVVSEHFHISVDQMISKTRSSEIARPR
                     QIAMYLCKTMTSDSLDVIGQLLGGRDHSTIIHGIKKVTKDYEENDSTRTLIETIKKKI
                     NPN"
     gene            5152..>6182
                     /locus_tag="DHV81_07875"
     CDS             5152..>6182
                     /locus_tag="DHV81_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCI94364.1"
                     /translation="MKLICSKANLLKGVNIVSKAVPTRTTMAILECILIDASANEIKL
                     MANDMELGIETIIDGTIEERGIIALDAKIFSEIVRKLPDNDVTIETDASFKTVISCEK
                     AKFNIIGKSGDDFSYIPYVERNESIVLSQFTLKEVIRQTIFSIADNDNNKLMTGELFE
                     IEENKLRVVSLDGHRISIRYIEMKNHYDSKKVVVPGKTLQEISKIIPGSADEDVVIYI
                     TNNHIVFEFENTTVVSRLIEGEYFKIDQMLSSDYDTKVRINKRELLECIDRATLLVKE
                     GDKKPIIMNITDGNMELRINSFIGSMNEDIDIDKDGKDIMIGFNPKFFIDALRVIDEE
                     EVNLYMVNP"
BASE COUNT         1835 a         1264 c         1076 g         1893 t
ORIGIN      
        1 ttcatcaaaa gcaccataac ctcatctatt ttctcatcgc cgtcatatat aaaaccataa
       61 tgaatggtat gtgactgaca ggcgcttatt ttctttttat tattcttgct tttataaatc
      121 ctgtctatta ccgaaagtgc atcatctccg cttatcctga taattccgat tcctgacgga
      181 gacatagctg tggcaatagc cgctattgta tcattttgca tatgaaattc ctcaaaaatt
      241 ctaagataat atatattcaa aaaggatacc tgaaaccaga taccctttta atgatttata
      301 tctattattt cttaagtgtc acaacaacat gtctgtaagg ctcatcaccc tcgctgtgtg
      361 tttcaacaaa tctatcattc tgaagtgcag aatgaatcac tcttctctca aaaggattca
      421 tcggctcaag agaaacaggt ctctttgtac gcttaacctt gaatgaaata ttcttggcaa
      481 gattctcaag tgtctctctt cttctctttc tgtaatcctc agtatcaatc tttactctgt
      541 agtaattctc agactgctta ttgatagcaa gatttgtaag gtactggagt gaatcgagag
      601 tctgacctct cttgccgatt aaaacgccca tctcaggacc gttaagctca acatcaacat
      661 ttctgttgtc ctcatccact ttgatatcaa tagtaacctc aagcttcatt gccttaaaca
      721 cactgtcaag gaattcgcga atctcatcct caacacttgc ctcggccttt ttaaccttga
      781 tgacagcatc acggctgcca attccaagaa aaccattgct tcccttatca attacctcat
      841 actcgagctt atcgcttgtt gttccaagtt taatagatgc ctctgtcaaa gcatcgtcaa
      901 ttgtttttgc agatacttca atgtattcca ctattttctc cccctatttg cgtgaattgc
      961 gttcattaaa ctctttaacc atattagcct ttgaagcaag tgagcctgcc tttgcattag
     1021 ccttgtactc agcggccttt gcaagcttct cctctgtgcc tgctgataca gatacgtttg
     1081 ccttattgtc aattgacttt gtcttaatct gagctgcctt ctttatataa tcctcagaca
     1141 gtcccatttt ttctctcttc ttctttgcct tttcctcgtt ctttgccata acagcctcaa
     1201 gatcaagatt ctctatatgt ctgttgacga agaactgctg gatacctctt acaagtgcag
     1261 agcagatcca gtaaatacca agacctacag gaactgtaaa gcagataaca aatgacataa
     1321 atggcatcat gagattcatc atcttcatct ggcttgccat ctgatcattg ccgtttgtag
     1381 cctgtggcat gagcttgata ttaagcatct gtgtcagata tgacagtaca ggaataagca
     1441 gtgcaagtat tacaaaaagt aaccacttat caggcttgtc cgtaaagttg ctttttatga
     1501 tataccaagg tgtatctgag atattcagtc ccaaaaagta gttgaaatga agtaacgcat
     1561 cgtgcgtctt ctcaactgcg ctctggatac catcaaaaaa cttaccgtcc ataagagcat
     1621 cccagccctt tgaaggaagc ttataaagga tatcaatgat ataattgctt aaagtatcac
     1681 cacttgcatc ggcaagcttt gaagccgcat ttgaagcgtt aagaccgctt gatgtaacaa
     1741 cattatactt ttccatcaga ctgacaagtg tattctgata tccgcttgtc tgctgaattg
     1801 aatcaacaag ccctgtaaaa gagctcttaa ctccgctcag ataagcagga atattgtaga
     1861 acacacggta cagtgcaaac agaataggca tctgtataag catctgcaca cagcttccca
     1921 tcggagaaat accgtacttc tggtataaaa gctgtgtctc ctcctgcatg gccatcattg
     1981 aagcctgatc ctttttattt ttatatttag cctggatagc ctgcatctca ggctgcattt
     2041 tctgtgatag cttagaaaac ttctgctgct taatagtcag tggcaacaga cacatgtaga
     2101 ttataatcgt aataattaca atacttagta caacgctctc aactcttcca cctgtaatat
     2161 tagatatacc actgtatatc caatccatca ggataccgag aagcttggca attggtccaa
     2221 gaatagagcc tggatatgcg gttaataaaa tgtccgacat aaaaacctcc attgctgcta
     2281 tggaaccgga tcatacccac cctttgaaaa aggattacat ctcaatatcc tccatgccgc
     2341 aagcagacca ccctttatgg caccgtactt ttgcacagcc tgcaaaccat actccgagca
     2401 ggtcggacaa tatgggcatt tcgttgattt catcggggaa atatacttcc tataaaattt
     2461 aatcaaaaat attaagatac ttttcaaaac gattccatct tctttttcat tattcaaata
     2521 ttttgtttct taataatcct gtgaagacct ccaagatgta aaacagcact ttctatttcc
     2581 ttataaccca catgccgcgc tgcaactctt gcgatgacta cgatgtccaa accactatcg
     2641 aacatgtcct catgtagtct gtagctctct ctcacgagac gagtaatatg atgtctaata
     2701 acactatttc caactttttt gctaactgat atacctatac gattattttc tgtgttattt
     2761 ttcagaacat acataacgag gtatctgtta gcaaacgata caccgttatt atacacattc
     2821 tgaaaatcct tgtttttttt caacgaatct gaaaatttca tttgacgctg tccttctaaa
     2881 aacaatcaaa aatgaagaaa agaccacaaa aattgtggcc tatgctgata atttttttct
     2941 tccttttaat cttctggctg cgattacttt tcttccgcca gcagttttca ttctctgtct
     3001 gaatccatga acttttgctc tctgtctctt cttaggttgg aatgtcatct tcatatatat
     3061 ccacctcctt tacgttaaaa aacatcggta taattataac aaaaattcct gttgtgtcaa
     3121 ggattttctc aattttcttt aaatttctac atatgttttt ttatcggttt atattccaca
     3181 ataaaaatgt ggaaaacgag tttcttctat atatataata attcttgata accgtctata
     3241 ttttgatata aaccacctat tgtgaccaac atattgtgga aagttatcaa gatatcaaaa
     3301 agttatccac aatatgtgaa taacatgttg gtaactttgt ataattgtgg ttnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaat atactcttta
     3481 tatgttaaat aatggagaac tttgatggat aaagtaatgg aaaagtggaa tgaaatacta
     3541 cagatggtca aggaagagca tggtctgaca gacgtttcct ttaatacgtg gataaaaccg
     3601 cttgaggtgt tcgccataga cggcaataca ctgtatattc tcgttccgtc tgagcagatg
     3661 gggctcagct atatcaacaa aaaatattat cttcctctta aggtagctat aggtgaggtg
     3721 acaggtatcg agtatgatat ccaatttatt ttaccggatc aggctcagag ccttaagttt
     3781 aaaaacaacg atacccctca gcttgagcca cagactcctg taaaggggga tcactcaaac
     3841 ttaaacccta actatacatt tgacaccttt gtcgtaggta ataataacag gtttgcccat
     3901 tctgcttctc ttgcagtggc agaaagcccg ggtgaagcat acaatccgct atatatatat
     3961 ggtggtccgg gacttggcaa gactcatctt atgcattcaa taggtcattt catcattgac
     4021 cagaatcctg ataccaaggt gctttatgtt acttctgaag agtttaccaa cgaggttatc
     4081 gaatctatac gtaacggtaa ttcttctgcc atgacaaagt tccgtgacaa atacaggact
     4141 gtagatgtgc tcatgattga tgatatccag ttcatcatag gaaaggaaag tactcaggag
     4201 gagtttttcc acaccttcaa cacactgcag acacagggca agcagattat ccttacctct
     4261 gataagccgc ctaaggagat ggagactctc gaggagcgca tccgttcccg tttcgagtgg
     4321 ggactgatgg ctgatatcgg tactcctgac tatgagacga ggatggctat cctccgtaag
     4381 aaagctgaaa ctgacaattt tgatatagat gatgatattt taaactacat tgcttcaaac
     4441 atcagctcca acatccgtga gcttgaggga gccttaaaca agcttttagc cttccacaat
     4501 ctcgagcata ctcacatcac tatggatatc gctgagaggg agctttccaa tatcatcacg
     4561 ccggacaagc caagggaaat cactgcacag ctcatcattg aggtcgtttc agagcatttc
     4621 cacatctctg tggaccagat gatttcaaag accagaagca gcgagattgc acgtccaaga
     4681 cagattgcca tgtacctctg caagaccatg acctctgatt cacttgatgt aatcggccag
     4741 cttttgggcg gcagagacca ctctactatc atccacggca tcaagaaagt caccaaggat
     4801 tacgaggaga atgacagtac acgtactctc atagaaacta tcaagaaaaa gattaatcca
     4861 aattagtttc tacctattta tatatgtgct catttgcatg ttaataattt ggttataacc
     4921 ttgcttttat ttccacatga gcatgttaat gacaggttga aggaaagtga ataaaaagtt
     4981 atcaattttt atcattttta cttcaacagg tttttaagca agttatcaag ccgcttaaag
     5041 cttgtcttta aaggctttga aggggtttta aactttttaa cagcccctaa gaagaatatt
     5101 acgaatatct tttaaatatt tatatatacg cgcaggaagg agtttttcta aatgaaactt
     5161 atttgttcaa aagcaaattt actgaaaggt gttaatatcg tatcgaaggc agtacctaca
     5221 cgtactacaa tggcaattct cgagtgcata cttattgatg catccgcaaa cgagattaag
     5281 cttatggcta acgatatgga gcttggaatc gaaacaataa tagacggaac tatcgaagag
     5341 agaggaatta tcgctcttga tgccaagatt ttttcagaaa tagtgagaaa gcttcctgac
     5401 aatgatgtaa caattgagac agatgcttcc ttcaagaccg tcatatcatg tgaaaaggcc
     5461 aagttcaata tcatcggaaa atcaggtgat gatttttctt acataccata tgttgagaga
     5521 aatgagtcta ttgtcctctc acagtttacc cttaaagagg ttatcagaca gacaattttc
     5581 tcaattgctg acaatgacaa caataaactg atgacaggtg agctttttga gattgaggag
     5641 aataagcttc gagttgtttc tcttgacgga cacagaatat ctatcagata tatcgagatg
     5701 aaaaatcact atgacagcaa gaaggttgtt gttcccggca agactctgca ggaaataagc
     5761 aagattattc cgggttcagc ggatgaggat gtggttatct atatcactaa caatcatatt
     5821 gtttttgaat ttgaaaatac tactgttgtt tcacgtctta tcgagggtga gtatttcaag
     5881 attgaccaga tgctttcatc tgactacgac actaaggttc gcatcaataa gcgtgagctt
     5941 cttgaatgta tcgaccgtgc tactcttctt gtcaaggagg gagataagaa gcctattatc
     6001 atgaatatca cagatggcaa tatggagctt cgcatcaatt catttatcgg ttccatgaat
     6061 gaggacatcg atatcgacaa agatggaaaa gatattatga taggctttaa tcctaagttc
     6121 ttcatagatg cacttcgtgt tatcgatgag gaggaggtta atttatacat ggtcaatcca
     6181 aa
//
LOCUS       DPUF01000223            3206 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1228, whole
            genome shotgun sequence.
ACCESSION   DPUF01000223 DPUF01000000
VERSION     DPUF01000223.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1228"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     assembly_gap    383..472
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            562..945
                     /locus_tag="DHV81_07880"
     CDS             562..945
                     /locus_tag="DHV81_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCI94365.1"
                     /translation="MSLIKGIHHVSMKCSSTEEYEKTIDFYKNILGIPVAREWQAGIM
                     LDTGNGIVEIFNDGDDAPDKGVIRHFAFATDDVDACVEAVKAAGYEVFIEPKDIEIAS
                     TPVFPARIAFCKGPLGEEIELFEEK"
     gene            982..2235
                     /locus_tag="DHV81_07885"
     CDS             982..2235
                     /locus_tag="DHV81_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94366.1"
                     /translation="MGKTPVKHTLAGYIIKKTDTEGYRSGKLTGEKHFNKITEMMEFV
                     GGRRKLIDQARFIENNTQAGHDGKIRINWIQVNSDIKKIDCDVSSIPELCRLEGVEDS
                     RAKQVRLIESVKQWKSEVGDCDWICRYYDDILGRLKAGKSVTEAEEDMRFKCINSITR
                     IKEPVWERVFSANVFKDSKKFEKCYRQKMVSILTKYSPYYEKDMEDYDTEGEEDDAKE
                     DKKMSGLEILKMHGIMSYAQTMEWKGPLSYRIDDTCVIDTSKQIYGTIINTQTLEHAS
                     PVSLAGCKRIMTIENKANYESMQYDENTLYIFCHGYFTPKEVYFLKKLSLIVSKECEF
                     LHWGDMDFGGISIFLFIKDRIFEKLMPYRMGVADFEEALKKEAGIPLKASTREKLQKK
                     DAGLLTELKEAILESDKTIEQERLL"
     gene            2299..>3206
                     /locus_tag="DHV81_07890"
     CDS             2299..>3206
                     /locus_tag="DHV81_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94367.1"
                     /translation="MNPFKMRPERTGDLFVDWEKFWVKPYNKNEVNPYTRTRIILMNG
                     TEFENVWFSHQFSRSVGDDELRRKLAYIRKSEQQQQKILTHLKPADESALEHTIGYEQ
                     LAVDLTAHLAKRVNDKNIKSALDFALLEDFDHLYRYADYLDFTTGEHAEKLVGGYTEI
                     TPGRPTISHHRHPYDSIRYPMTDKCPATMDVLAANVITAAEQQTMNYYMNTAALWPDE
                     IGRRLYQEIGMVEEQHVTQYGSLLKPCMSRLENLLVHQYVECWLYWSCYETETDTRIR
                     GIWQFMFEQELKHLHIALELLRQYEK"
BASE COUNT         1081 a          496 c          747 g          792 t
ORIGIN      
        1 tagattgtac ttattttctg atactatgag tatatagtat aatgaagcgt acgaatttgc
       61 aaataaattt cgtaattgtt cagtgccttc atagctgcgt ggccgcaatc ataatcgtct
      121 gtatatatgg acaatagaaa attttataga tataatatga tttaagggat cctgaattgg
      181 ataccttttg actctcacat cattttacta agtaggacta aattggcata atgtacaata
      241 gccataggtt gatagcatag taggcaggca ctggggagct gtaattccta aaacttttaa
      301 aacagcaccc aaaattcagc ccaaaggccg gaaaaatagc gagaaaagta gctgaaactg
      361 cagaaatctc caaaacagct ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcccaaaa
      481 gccggaaaaa tagcgggaaa aagtagctga aatcactata aatcacaaaa cagctcaaaa
      541 aaattaaagg gagaaaacac aatgagctta ataaaaggta tacatcacgt atctatgaag
      601 tgcagcagca cagaggaata tgaaaagaca atagatttct acaaaaacat tctgggaatc
      661 ccggtcgcaa gggaatggca ggcaggaatc atgcttgata ccggaaacgg aattgttgag
      721 atttttaacg atggagatga tgcaccggac aaaggtgtta ttcgtcattt cgcattcgca
      781 acagatgatg tggatgcgtg tgtagaggct gtaaaagcag caggatacga ggtatttatt
      841 gagccgaagg atatagaaat cgcatcaaca ccggtatttc cggcgcgcat agcattctgc
      901 aaaggaccac ttggcgagga aatcgagctg tttgaggaaa agtgatagaa cggattaatg
      961 gttttatagg ggtattatac tatgggaaaa actccagtta agcacacgct tgcaggctat
     1021 attataaaaa aaacagacac cgaaggatac cggtcaggaa aactaaccgg agaaaaacat
     1081 tttaacaaaa taacagaaat gatggaattt gttggtggac ggcggaaact aatcgatcag
     1141 gcaaggttca tagagaataa tacacaagca ggccacgatg ggaagataag aattaattgg
     1201 attcaggtca acagcgatat taaaaaaatt gattgtgatg taagcagcat accggagctg
     1261 tgtcggcttg agggagttga ggattcaaga gcaaaacagg taagattgat agagtctgta
     1321 aagcagtgga aatcagaggt tggtgactgc gattggatat gcagatatta tgatgatatt
     1381 ttggggcgtt tgaaagccgg aaagagtgtc acagaggctg aggaggatat gcgttttaag
     1441 tgtatcaatt ctataacaag aataaaggag cctgtgtggg agcgtgtttt tagtgcaaat
     1501 gtttttaaag attcaaagaa atttgaaaaa tgctatcgac aaaagatggt atcaattctg
     1561 actaaatatt ctccatatta tgagaaggat atggaggatt atgatacaga gggagaggaa
     1621 gatgatgcca aagaggataa aaagatgtcg gggcttgaaa ttttgaagat gcatggcatt
     1681 atgtcatatg cacagacaat ggaatggaag ggaccattgt catatagaat tgatgataca
     1741 tgtgttattg atacgtcaaa gcagatttat ggaaccatta ttaatacgca gacacttgag
     1801 catgcaagtc cggtttcgct tgcaggatgc aaaagaatta tgaccataga aaataaggct
     1861 aactatgaaa gtatgcaata tgatgagaat accttgtata ttttttgtca tggttatttt
     1921 actcctaagg aagtgtattt tctaaagaaa ttaagcctta ttgtatctaa ggaatgtgag
     1981 ttcctccatt ggggagatat ggattttgga gggataagca tattcttgtt tattaaggat
     2041 agaatttttg aaaaactaat gccttataga atgggggtag cggattttga ggaagcatta
     2101 aaaaaagaag ctggaatacc acttaaggct tcaactaggg aaaaactgca aaaaaaggac
     2161 gcaggattgc ttactgaatt aaaagaagca attcttgaat cggataaaac aatagagcag
     2221 gaaaggctct tatgagattc atacgcacag catacctcct tcgtgcatac aatagcatga
     2281 aggaggtatg tttgtattat gaatccattt aaaatgaggc cggagagaac cggcgattta
     2341 tttgttgact gggagaaatt ctgggtaaaa ccatataaca aaaatgaggt aaatccgtat
     2401 acaaggacga gaatcatatt gatgaatggt acagaatttg aaaacgtgtg gttttcacat
     2461 cagttttcaa gaagcgtagg tgatgatgag cttagacgaa agcttgcata catcaggaaa
     2521 agtgagcaac agcagcagaa aatactcaca catttaaagc cggcagatga gtcggcattg
     2581 gagcacacca taggctacga gcagcttgcg gttgacctga ctgctcatct tgcaaaacga
     2641 gtaaatgaca aaaatattaa gtctgcgctc gattttgcct tgctcgagga ctttgatcat
     2701 ttgtaccgct atgcagatta tcttgatttt accacaggtg agcatgcaga gaagcttgtc
     2761 ggaggctata cggaaatcac accgggcaga ccgactattt cacaccacag gcatccatat
     2821 gattcaatac gatatccgat gacggataaa tgccctgcga caatggatgt gctggctgca
     2881 aacgtaatca cagcagcaga acagcagact atgaactact atatgaacac agcggcactt
     2941 tggcctgatg agattggacg caggctatat caggaaatcg gaatggtcga ggaacagcat
     3001 gtcacacagt atggcagcct gttgaagcca tgcatgagcc gccttgaaaa tctccttgtg
     3061 catcagtatg tcgagtgctg gctgtattgg agctgttatg aaacggagac ggatacaaga
     3121 atacgtggca tatggcagtt tatgtttgaa caggagctta agcatctgca tattgcactt
     3181 gagctgctaa gacagtacga gaaaaa
//
LOCUS       DPUF01000224            5197 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_4855, whole
            genome shotgun sequence.
ACCESSION   DPUF01000224 DPUF01000000
VERSION     DPUF01000224.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_4855"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(1..773)
                     /locus_tag="DHV81_07895"
                     /pseudo
     CDS             complement(1..773)
                     /locus_tag="DHV81_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742531.1"
                     /note="too many ambiguous residues; incomplete; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP pyrophosphatase"
     assembly_gap    390..477
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(775..1365)
                     /locus_tag="DHV81_07900"
     CDS             complement(775..1365)
                     /locus_tag="DHV81_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCI94368.1"
                     /translation="MADRCAKLSRKTKETDISVGLNIDGTGKSNIDTGIGFFNHMLEG
                     FSKHGFFDLDMICDGDLNVDCHHTIEDCGIVLGNAIAQAVGDKKGIKRFGSFILPMDE
                     VLVLCAIDLSGRPYLQFDGEFTCERVGDMDTEMVREFFYAVSYSAAMNLHIKVLTPGN
                     NHHMIEAMFKAFAKALDDACTYDSRIKDVMSTKGSL"
     gene            complement(1381..2670)
                     /gene="hisD"
                     /locus_tag="DHV81_07905"
     CDS             complement(1381..2670)
                     /gene="hisD"
                     /locus_tag="DHV81_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCI94369.1"
                     /translation="MRILKLTKDSQKNILESLLKRSPNNYTEYESTVNEIINNVKENR
                     DKAVFEYTKKFDKADVDASNIRVTEEEIKEAYELVDEKLLAVIKKALVNIRKYHEKQL
                     QNSWFTTEDGIILGQKVTPIAKAGVYVPGGKAVYPSSVLMNVLPAKVAGVEKIVMCTP
                     CGADGKVYPSTLVAANEAGVDEIYKVGGAQAIAAMAFGTESVPKVDKIVGPGNIFVAL
                     AKKAVFGYVSIDSIAGPSEILVLADETANPRYVAADLLSQAEHDEMASAILITTSEEL
                     AKKVSEEVDKFAEVLSRKEIIEKSLENYGYILVAEDMDEAIDAVNDIASEHMEIVTRD
                     PFSVMTKIKNAGAIFIGEYSSEPLGDYFAGPNHVLPTNGTAKFFSALSVDDFIKKSSI
                     ISYSREALERIHTDIEQFAECEKLTAHANSIKVRFED"
     gene            complement(2713..3360)
                     /locus_tag="DHV81_07910"
     CDS             complement(2713..3360)
                     /locus_tag="DHV81_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCI94370.1"
                     /translation="MSDDRYLTFALGKGRLAKKTLQLFEQIGITCEEMKDPDTRKLIF
                     VNEELKLRFFLAKGPDVPTYVEYGAADIGVVGKDTILEEGRNIYEVLDLGFGKCRMCI
                     CGPESARELLQHHEQIRVATKYPHIAKDYFYNKKHQTVEIIKLNGSIELAPIVGLSEV
                     ICDIVETGTTLKENGLTVLEEVCPLSARVVVNQVSMKMDNERITKLISDLKTVIK"
     gene            complement(3364..4554)
                     /gene="hisZ"
                     /locus_tag="DHV81_07915"
     CDS             complement(3364..4554)
                     /gene="hisZ"
                     /locus_tag="DHV81_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCI94371.1"
                     /translation="MENQLLHTPDGVRDIYNGECKKKLYLQDKLHRTLLKYGYHDIMT
                     PTFEFFNIFGSDVGTTPSKDLYKFFDKEGNTLVLRPDFTPSIARSAAKYYIEDDMPVK
                     LCYLGNTFINSSDYQGRLKESTQCGAELIGDGSISADAEILSMTVESMLESGLKNFQI
                     SVGHSQFFYGLTKAAGLSGEQEENLRELIANKNFFGVEEFVDSLSMNDKLRKLFGLLD
                     NFDLQDEQLMEALKLANGYDEILSSIDTLLKLREYLKAYGIEKYISYELGLISDYTYY
                     TGIIFQGYTYGTGEPIVKGGRYDKLLSHFGKDAAAIGFAIVVDQLMAALSRQDIDVPV
                     IENSSLIVFEEAAYHAAIKRSMELRRDGVNTQMVLKDKNKTKEDYASYAVDNRINRVE
                     FIEE"
     gene            complement(4627..5160)
                     /locus_tag="DHV81_07920"
     CDS             complement(4627..5160)
                     /locus_tag="DHV81_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCI94372.1"
                     /translation="MATWNSRGLRGSTLEDMINKTNEKYMENGLALIQKIPTPITPIK
                     MEPETRHITLAYFEQKSTVDYIGAVQGIPVCFDAKECNTDTFPLANIHEHQVEFMRCF
                     EKQDGVAFILISYTHRNEYYYLTFKKLTEFWDRAAEGGRKSFRYEELEPAFFLPEVPG
                     ILVPYLNALQLDLNCRA"
BASE COUNT         1350 a         1244 c          899 g         1616 t
ORIGIN      
        1 gcaagcatga gcacctgctc attgcggtaa tcctgcacta tgacaggcac catgccgtca
       61 gaattaagct ttaaatcaga ccattttaag tcgggagtaa aattgtcaac aagtattcct
      121 ccgtcagaaa gctttgtctt taacgccatg atatcatttt caggatcatt gataaactct
      181 cctgcaatgc ctcttagtgt ttcacgcttc atgacatcaa ttattttatc catgtcaaac
      241 tgcggcatat agaccacata cggtgtggat gtaagattct caagggcatc tattatatct
      301 atatttaaga ccaaaagctc atgaaaggta tcctctatct cctcctgatg cttgaaaata
      361 tagtctacat tgctgattga caaaagaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc
      481 tgtaaacagc ctgcatacag cagctttttc acgtcctcta tgcgattgat attgcctccg
      541 gcgcacacct ttatgtcgat atttctgttt atattttcta tcgtatgtat gtttttctca
      601 tgctcatcat catcagttga aagatcaaaa ataataatct tatctatgcc actgtcattg
      661 tagagctgac acagattgta gacgtcgccc ataactgtca tatcagacgc tgatttcacg
      721 gcctggccgt tttttaaata cagtgtagca acgatgttct taaaaccagt caaattacaa
      781 acttcctttc gtggacatca cgtcctttat tctgctatca taggtgcagg catcgtcaag
      841 cgccttggca aatgccttaa acatagcttc tatcatatga tgattatttc ccggtgtcaa
      901 aacctttata tgcaggttca tcgcagcaga gtaagaaacg gcatagaaaa actctctcac
      961 catctctgta tccatatctc cgactctctc acaggtaaac tcaccgtcaa actgcaggta
     1021 cggtctgcct gataaatcaa tggcacatag tacaagcacc tcatccatcg gtaaaataaa
     1081 gcttccaaat ctctttatgc ccttcttgtc tccgacagcc tgtgctatgg cattgccaag
     1141 cactatgccg cagtcctcta tagtatgatg acagtccaca tttaagtcac cgtcacatat
     1201 catatcgagg tcaaaaaaac catgctttga aaagccctcc agcatatggt taaagaagcc
     1261 tatgcctgtg tctatatttg attttccggt tccatctata tttaaaccga ccgaaatatc
     1321 agtctctttt gtttttctgc taagcttagc gcatctatct gccatttctt actcctttga
     1381 ctaatcctca aatctaacct tgattgaatt tgcatgtgcc gtaagcttct cacactctgc
     1441 aaactgctca atatctgtat gtattctctc aagtgcttct cttgaatatg agatgatgct
     1501 tgactttttg ataaagtcat ctacagataa ggctgagaag aattttgctg ttccgtttgt
     1561 aggcagaaca tggtttggtc ctgcaaaata gtctcccaaa ggctctgatg agtactctcc
     1621 gataaagatt gctcctgcat ttttgatttt tgtcatgact gagaaaggat ctcttgtgac
     1681 aatctccata tgctctgaag cgatatcgtt tactgcatca attgcctcat ccatatcctc
     1741 tgcaacaagt atatatccgt agttttcaag agatttctca atgatttctt ttcttgagag
     1801 gacctcagca aacttatcaa cttcctctga tactttcttt gcaagctcct cacttgttgt
     1861 gatcaagata gctgatgcca tctcatcatg ctcggcctgt gaaagtaaat ctgctgccac
     1921 atagcgtgga tttgcagtct catctgcaag caccagaatc tcgctaggtc ctgcgattga
     1981 atcaatgctg acatatccaa acacagcctt tttggcaagg gcaacaaaga tatttcccgg
     2041 tccaacaatc ttgtctacct ttggcacact ctctgtacca aatgccattg cagcgatagc
     2101 ctgtgcaccg cctaccttgt agatttcatc tactccggcc tcgtttgcgg ctacaagtgt
     2161 ggaaggatat accttgccgt cagctccaca tggtgtacac ataacaatct tttcaacgcc
     2221 tgctaccttt gcaggcagca cgttcatcag cacagatgaa ggatagacag ccttgccgcc
     2281 cggcacatat actcctgcct ttgctatagg tgtgaccttc tgtcctaaaa tgatgccgtc
     2341 ctctgttgta aaccatgagt tctgcaactg cttctcgtga tattttctga tatttacaag
     2401 agcctttttt ataacggcaa gaagcttctc atcaaccagc tcatatgctt cctttatttc
     2461 ttcctctgtg acacggatat ttgatgcatc cacatccgct ttatcaaatt tctttgtgta
     2521 ttcaaaaaca gccttgtcac ggttttcctt tacattgttt ataatctcat ttaccgtgct
     2581 ctcatactct gtatagttgt tagggcttct cttaagcagg ctctccaata tgttcttctg
     2641 tgaatccttt gtaagtttta aaattctcat tacagcctcc gtctaaaatc atgtattgat
     2701 ataaattata cgctatttga taacagtctt caaatcactt atgagctttg tgatacgctc
     2761 attgtccatc ttcatgctga cctggtttac cacaaccctc gcagacagcg gacagacctc
     2821 ctcaagcacg gtaagtccgt tctccttaag tgtagttccg gtctctacta tatcacaaat
     2881 cacctctgaa agtccaacaa tcggagcaag ctctattgag ccgtttagct ttataatctc
     2941 taccgtctga tgctttttat tgtagaaata atcctttgcg atatgaggat acttggtggc
     3001 aactcttatc tgctcatgat gctgcaaaag ctctcttgcg ctctccggtc cgcatatgca
     3061 catgcggcat tttccaaagc ctaaatcaag cacctcgtat atatttctgc cctcctcgag
     3121 gatggtatcc tttcctacca caccgatatc ggcagcgcca tactccacgt aagtaggcac
     3181 atcaggtccc tttgccagga aaaatctaag cttaagctcc tcatttacga aaatcagctt
     3241 tctggtatcc ggatccttca tctcctcaca ggtgatgcct atctgctcaa aaagctgcag
     3301 ggtcttcttt gcaagtctgc cctttcccag ggcaaaagtt aaatatctgt catcactcat
     3361 cttttattcc tctataaact caactctgtt tatacggttg tctaccgcat atgaagcata
     3421 gtcctctttc gttttatttt tgtccttaag caccatctgt gtatttactc catctcttcg
     3481 aagctccata gagcgcttga ttgccgcatg gtatgcagcc tcctcaaata ctataagtga
     3541 ggaattctca atgacaggca catcaatatc ctgacggctt aatgctgcca tgagctgatc
     3601 cacaacaatt gcaaagccga ttgcagcagc gtcctttcca aagtgtgaaa gcagcttgtc
     3661 ataacggcct ccctttacaa taggctctcc tgtaccatat gtgtagccct ggaaaataat
     3721 acctgtgtaa tatgtgtagt cactgataag tccaagctca tacgaaatat atttctcgat
     3781 gccgtatgcc tttaaatatt ctctaagctt aagcagagta tcaatagatg ataatatctc
     3841 atcatatcca ttcgcaagct ttaaagcctc cataagctgt tcatcctgta aatcgaaatt
     3901 gtctaatagt ccaaacaact tccttagctt gtcgttcatt gaaagactgt caacaaactc
     3961 ctccacaccg aagaaattct tatttgctat gagctctctt agattttcct cctgctcccc
     4021 tgacagccct gctgcctttg tgagaccgta aaagaactgc gaatgtccga ctgaaatctg
     4081 gaaattctta agtccgctct caagcatcga ctcaactgtc atcgaaagta tctcggcatc
     4141 tgcagagata gagccatcac ctatcagctc tgcaccgcac tgtgtgcttt ccttgagcct
     4201 accctgataa tccgatgaat tgataaaagt attgccaaga taacacagct tgacaggcat
     4261 atcgtcctct atatagtact tagccgcact tcttgcaata gaaggtgtga aatcaggacg
     4321 aaggacaagc gtattgccct ccttgtcaaa aaacttgtat aaatcctttg aaggggttgt
     4381 tccgacatcg cttccaaaaa tattaaaaaa ctcaaacgta ggtgtcatga tatcatgata
     4441 tccgtatttt aaaagtgtac ggtgcagctt atcctgcaaa tacagctttt ttttgcattc
     4501 tccgttatat atgtctctta ctccgtccgg agtgtgtaat aattgatttt ccatattgtg
     4561 taaaatctcg ctttcatgta gtaaagtgtt atcgtggtaa tatgctacca tacgatagta
     4621 atactgtcaa gccctgcaat ttaagtcaag ctgaagtgcg ttcagatacg gcacaagtat
     4681 tccgggcacc tccggaagga aaaatgccgg ctcaagctcc tcatagcgga agctctttct
     4741 gccgccttct gcagctctat cccagaattc tgtcagcttt ttaaacgtga gataatagta
     4801 ttcgttcctg tgtgtgtacg atataaggat aaatgcaaca ccatcctgtt tttcaaagca
     4861 tctcataaac tctacctgat gctcatgtat attggcaagg ggaaacgtgt cggtattaca
     4921 ttcttttgca tcaaagcata ccgggattcc ctgtacagcg ccaatatagt caaccgtact
     4981 cttctgctcg aaataagcca gtgtaatgtg tctggtttcc ggctccatct ttataggtgt
     5041 aatcggagtg ggaatctttt gtatgagcgc caggccgttc tccatgtatt tttcatttgt
     5101 cttgtttatc atgtcctcta aggtcgagcc tcttaagcct cttgaattcc aggttgccat
     5161 atcgtttcta tcttccttaa tactgtaagc tatgcga
//
LOCUS       DPUF01000225            4709 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_33102, whole
            genome shotgun sequence.
ACCESSION   DPUF01000225 DPUF01000000
VERSION     DPUF01000225.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_33102"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="DHV81_07925"
     CDS             complement(<1..649)
                     /locus_tag="DHV81_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94373.1"
                     /translation="MIDIVTNNVRQIGTPADEGRVYISEKAYKRIKTEGFKDKEVFVL
                     MGHTESSGGRYATFVEAAIAVYDMEFDKNAPLWTNKVWSRVFAEIKKSYEELIIVGWA
                     FRQEDFPAEPTPMLENIHREHFGGAHQLMFLLNRTEQEENFFIKKNNHLKKKTGFFVY
                     YRMNEHCDSADSYNETDTCNKTCSYSNANNNNKIDSYSNADDNNKSSSRYEDTDTA"
     gene            complement(1027..1968)
                     /locus_tag="DHV81_07930"
     CDS             complement(1027..1968)
                     /locus_tag="DHV81_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HCI94374.1"
                     /translation="MSEVKKIATRDSYGNALVELGKAHDDLIVLDADLAGATKTCIFQ
                     KEFPERHWDCGIAECNMTGIAAGLATCGKVPFISSFAMFAAGRNYDQVRNSIGYPHLN
                     VKIGATHAGISVGEDGATHQCLEDLSLMREIPGMVVINPSDDVEARAAVKAAYDHVGP
                     VYLRFGRLAVPVINDTPDYKFEIGKGIVLGEGKDVSIFATGLEVSETLEAAKMLAADG
                     IDAEVINIHTIKPIDRELIVKSVSKTGKAVTVEEHSINGGLGSAVAEVLCEEQPAKLL
                     RIGVEDRFGESGPAVELIHKYGLDAEGIYNKVKNYMK"
     assembly_gap    1977..2138
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2139..2803)
                     /locus_tag="DHV81_07935"
     CDS             complement(<2139..2803)
                     /locus_tag="DHV81_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCI94375.1"
                     /translation="MNNLELEKMANEIRKDIVTAVHSAKSGHPGGSLSSADIFTYLYF
                     EEMNVDPANPKWEDRDRFVLSKGHVAPGLYSTLAEKGYFPKEDLKTLRHTGSYLQGHP
                     DMKHIPGIDMSSGSLGQGVSVAVGMAAAGKYDKKDYRVYTLTGDGEIQEGQIWEAAMW
                     AGHRKLDNLVVIVDNNNLQIDGSVEDVCSPYPIDKKFEAFNFHVINIDGNDFDQIRAA
                     FKEA"
     gene            complement(2822..3418)
                     /locus_tag="DHV81_07940"
     CDS             complement(2822..3418)
                     /locus_tag="DHV81_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCI94376.1"
                     /translation="MDFYSSEISKLIEELSQLPGIGAKTAQRLAFHIINMPKEQAKSL
                     SDAIIDAKEHVRYCKCCYTLTDDELCPICKDAARDHKTIMVVENTRDLVAYEKTGKYE
                     GVYHVLGGAISPMLGIGPADIRLKELMVRLEKDVNEVIIATNSSLEGETTAMYISKLI
                     KPTGIKVTRIASGVPVGGDLEYIDEVTLLRALQGRTEI"
     gene            complement(3418..3759)
                     /locus_tag="DHV81_07945"
     CDS             complement(3418..3759)
                     /locus_tag="DHV81_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCI94377.1"
                     /translation="MARRGGFPGGMPGNMNNLMKQAQKMQRQMEEAQKQLEDAEVTAK
                     AGGGAVEVTVSGKKEITKVKLSEEVVDPDDIEMLEDLIMAATNEALRQIDEQSQASMS
                     KITGGLGGGLF"
     gene            complement(3810..>4709)
                     /locus_tag="DHV81_07950"
     CDS             complement(3810..>4709)
                     /locus_tag="DHV81_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCI94378.1"
                     /translation="IAFHLGEKLTYENVLEVLGAVDTEVFSRLLRRILDEDVTGAIRI
                     LGELIDEGRELSQLVNDFTWYMRNLLLLQSSDDMEEVLDISKDNLEALKEEAALVKPE
                     TLIRYIRVFSELSSQVKFASQKRIMVEIAIIKLCRPQMEKSYDSVLDRINSIEKKLEK
                     GIPVAAQAAQTQDMQQAAASGPVVKKELPKAIPQDISELMKHWKSILSEMGATSKVYL
                     NKAVTSLGNSSDLILMFDDENAYEYLSENRAGCMDTLASLIEKRIGKKVEVTVKKNNS
                     AVSAKEAVVDLTDMINFDIEEEN"
BASE COUNT         1040 a         1227 c          900 g         1380 t
ORIGIN      
        1 ccgctgtgtc agtgtcctca taccggctgc tgcttttatt attgtcatcg gcattactat
       61 aactgtctat tttattattg ttgtttgcat tactatagct gcaggtttta ttgcaggtgt
      121 cagtttcatt atagctgtca gcactatcac aatgctcatt catgcggtaa tatacaaaaa
      181 atcctgtctt tttcttcaga tgattattct ttttgataaa gaagttttcc tcctgctcgg
      241 tcctgttcaa aaggaacata agctgatgag cgccgccaaa gtgctccctg tgtatatttt
      301 caagcattgg tgttggctca gccggaaaat cttcctgcct gaatgcccat ccgacgataa
      361 tcagctcctc gtagcttttc tttatctccg caaacactct gctccatacc ttgttggtcc
      421 aaagaggcgc atttttgtca aactccatat catatactgc aatcgccgcc tccacaaatg
      481 tggcatacct gcctccgctg ctctccgtat gccccataag tacaaaaacc tctttatcct
      541 taaagccctc cgtctttatg cgcttgtacg ctttctccga tatgtaaacc cggccctcat
      601 ccgccggtgt gccaatctgc ctgacgttgt tcgtcacaat atctatcatg ccagcctcct
      661 ttttcggtca gcatagcact tgcaaaatgt aaatttatgc aaaattgtgc gattataaag
      721 gtattttgca gttaatcccc aacgaaaaaa ggacgcatga acccatgcgc ccgacttttc
      781 ccatatgaag cagtcaattg cttcgcactc gacaccctat aatccaccgg aaagcgtaca
      841 gtatattctg aagaaaacaa tatgaactgc cgcagggttc cattatgtaa aatagcaaga
      901 atgactaaac tcattcaaca cgctatccac acgaaatcct gcggctgact tcatattttt
      961 tctgaggaat atactgtatg ctgcaccggt gaacacagcc tccaagtgcg aagcacataa
     1021 aacccactac ttcatataat tctttacttt gttgtagatg ccctctgcat ccagtccata
     1081 cttgtggata agctccactg ccggtcctga ctcgccgaag cggtcctcta caccgatacg
     1141 cagaagcttt gcaggctgct cctcacagag tacctctgct acagcacttc cgagtcctcc
     1201 atttatagag tgctcctcta ctgtgactgc cttgcctgtc tttgatacag atttaacaat
     1261 cagctctctg tcgattggct tgattgtatg gatattgata acctcagcat ctatgccgtc
     1321 tgctgcgagc atttttgctg cctcaagtgt ctctgatacc tcaagtcctg ttgcaaatat
     1381 agatacatcc ttgccttccc ccagtacgat accctttccg atttcaaact tgtagtccgg
     1441 tgtatcgttg attacaggca ctgcaagtct gccgaatcta aggtatacag ggcccacatg
     1501 gtcatatgct gcctttactg cggctcttgc ctccacatcg tctgatgggt ttattacaac
     1561 catgcccggg atttctctca tgagcgaaag gtcctcgagg cactgatgtg tagctccgtc
     1621 ctcacctaca gagattcctg catgtgtggc acctatcttg acattgagat gaggatatcc
     1681 gattgagttt cttacctggt catagtttct gcctgctgca aacatggcaa atgagctgat
     1741 aaaaggcacc ttgccgcatg tagcaagtcc tgctgcgata cctgtcatat tgcactctgc
     1801 gattccgcag tcccagtgac gctcaggaaa ctccttctgg aatatacagg tctttgtggc
     1861 acctgccagg tcagcatcaa gtacaataag gtcatcgtgt gcttttccaa gctctacgag
     1921 tgcgtttccg tagctatctc ttgtagcgat tttctttact tctgacataa tgcttcnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ttccttgaag gctgccctga
     2161 tctggtcgaa atcgttgccg tcgatattga ttacgtggaa attgaatgcc tcaaacttct
     2221 tgtcgattgg gtatggtgag catacatcct caacagaacc gtctatctgg agattgttgt
     2281 tgtcaacgat aactacgaga ttgtcgagct tgcggtggcc tgcccacatg gctgcctccc
     2341 agatctgtcc ctcctgaatc tcgccatctc ctgtgagtgt gtatacacgg taatccttct
     2401 tgtcatattt tcctgctgca gccattccta ctgcgacaga tacaccctgt ccgagagagc
     2461 cgcttgacat atcaatgcca gggatgtgct tcatatcagg atgtccctgc agatatgagc
     2521 ctgtatgacg gagtgtcttt aaatcctcct ttggaaaata tcctttctct gcaagtgtag
     2581 agtaaagtcc cggtgctacg tgtccctttg agagcacgaa acgatccctg tcctcccatt
     2641 taggattggc agggtctaca ttcatctcct caaaatacaa atatgtaaaa atatctgctg
     2701 atgaaagtga tccacccgga tgtccggatt tggcactgtg aactgctgtc acaatgtcct
     2761 tgcgaatttc attcgccatc ttctcaagtt caagattgtt catgatgtct ccttctatca
     2821 tttatatctc tgttcttccc tgaagcgccc taagcagggt cacctcgtca atatattcca
     2881 aatcgcctcc tacgggaact ccgcttgcga ttcgtgtcac tttgatgccg gtaggcttga
     2941 tgagcttgct gatatacatg gccgtagtct cgccctccag gcttgaattg gtcgctataa
     3001 tgacctcatt tacgtctttt tcaaggcgaa ccatgagctc cttcagacga atatccgccg
     3061 gacctatgcc aagcattggt gaaattgcgc caccaagcac atgatacact ccctcatatt
     3121 taccggtctt ttcgtatgcc acaagatccc tcgtattctc aacaaccatg atggtcttgt
     3181 ggtctcttgc cgcatccttg cagatggggc atagctcgtc atctgtgagt gtatagcagc
     3241 atttgcagta tctgacatgc tccttcgcat cgattatggc gtcagagagc gattttgcct
     3301 gctcctttgg catatttatg atatgaaatg ccagtctctg cgctgtcttg gctccgattc
     3361 ccggaagctg tgacagctcc tctattagtt ttgatatctc actgctgtaa aaatccatta
     3421 aaacagaccg ccacccaggc caccggtaat tttggacata gatgcctgtg actgctcatc
     3481 aatctggcga agcgcctcat tggttgctgc catgatgaga tcctcgagca tctcgatatc
     3541 gtcaggatcc actacctcct cagataattt gacctttgtg atttcctttt ttccagaaac
     3601 agtgacttcg actgctccgc cgcctgcctt tgctgtaacc tcagcatcct caagctgctt
     3661 ctgagcctcc tccatctgac gctgcatctt ctgcgcctgt ttcataagat tgttcatgtt
     3721 tcccggcatt cctccaggga atccacctct tcttgccatt ctggcgtcct ccttgttttt
     3781 aatgggctaa tcccagccct gtttataact tagttttctt cctctatatc aaaatttatc
     3841 atatctgtca agtccacaac cgcttccttt gcactgactg cggaattatt tttcttgacc
     3901 gtcacctcga cttttttgcc tatacgcttt tctataagcg aagccagcgt atccatacag
     3961 cctgcacggt tttcagagag atactcatat gcattctcat catcaaacat taaaatcagg
     4021 tcagaggaat tgccaagcga tgtcacagcc ttgttcagat aaacctttga tgttgctccc
     4081 atctccgaaa gtatggattt ccagtgcttc atgagctctg agatatcctg tggtattgct
     4141 ttgggaagct cctttttcac tacaggcccg cttgctgcgg cctgctgcat atcctgtgtc
     4201 tgcgcagcct gtgccgctac aggtataccc ttttcaagct ttttctctat gctgtttatg
     4261 cggtcaagca ccgaatcata gctcttttcc atctgcggcc tgcacagctt tattatggct
     4321 atctcgacca taatacgctt ttgcgatgca aactttacct ggctggacag ctcggaaaat
     4381 accctgatat acctgatgag tgtctcaggc tttacaagcg ctgcctcttc cttaagtgcc
     4441 tcaagattgt cctttgagat gtcgagcacc tcctccatat cgtcggagct ctgcaaaagc
     4501 aaaagattcc tcatatacca ggtgaaatca ttgacaagct gcgacagctc cctgccctca
     4561 tctatgagct cgcccagtat gcggatggcg cctgtcacat cctcgtcgag tatgcgcctt
     4621 aagagccttg aaaacacctc tgtgtccacc gctccaagca cctcgagcac attttcatag
     4681 gtgagcttct cacccagatg gaaagcaat
//
LOCUS       DPUF01000226            7656 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16528, whole
            genome shotgun sequence.
ACCESSION   DPUF01000226 DPUF01000000
VERSION     DPUF01000226.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16528"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            192..1070
                     /locus_tag="DHV81_07955"
     CDS             192..1070
                     /locus_tag="DHV81_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCI94379.1"
                     /translation="MAERITGHTELIGLMAYPIRHSSSPAMHNEAFAYLGLDYAYLAF
                     EVDNNTLEDAVKGIRALKLVGSNVSMPNKTVVGQYLDKLSPAAELCGAVNTIVNENGV
                     LTGHITDGIGFMQALKDNDIDVIGKKMTIAGAGGAATAIEIQAALDGVKEISIFNIHD
                     KFWANAEETVRKINEKTDCKATLYDLDDKEKLREEMADSYIFVNGTGVGMKPLEGMSV
                     VPDKSFFRPELIVIDVPYSPLETKMRSMAKEVGCKTMNGLGMMLFQGAAAFKLWTGKD
                     MPIEHMKEVLNIRYDD"
     gene            1129..2340
                     /locus_tag="DHV81_07960"
     CDS             1129..2340
                     /locus_tag="DHV81_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI94380.1"
                     /translation="MNKKYLPSALILYLNYFIHGVGCSILGQAVIKEALAASWGVQVM
                     AITAISAALGLGRLIALPFAGPLSDKLGRRISTAIGSGSYAIYLIGLALAFNAGTHGG
                     YQIAYICAIIGGIANSFLDTGIYPAVSEIIYKAPGVATMGIKFFIAIAQMLLPFVLGM
                     TVATTAAGLTSYNTLFFGCGIIYIVLLVLVMIFPLPDSDAKAEGKKEGLIASLKHTHF
                     SVESIAMIIIGFTCTGTFQLWLNCAQNFAKENVGWADPSVMQSYYSAGTMIALIVTAL
                     LTRKIKDVRFILVYPVICLATMVVVLVGQTKPLMIAGAFIIGWAGAGGLLQIVTSVCN
                     MLFPKIKGTVTALVMIASSLCNYTILTAASKMQPSQVMVMNIVLTAIGVVLGLFVNLR
                     YTKMVELSKED"
     gene            2408..>3063
                     /gene="aroD"
                     /locus_tag="DHV81_07965"
     CDS             2408..>3063
                     /gene="aroD"
                     /locus_tag="DHV81_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCI94381.1"
                     /translation="MNTIKVRDIEIGAGAPKIIVPIVGVTKDDIIAEAKTFDSIPVDM
                     VEWRVDWFENVFEFDKVEEVLKELRDALGNIPILMTFRTSKEGGEKAIEPEAYAKLNI
                     KAAQTGYVDFVDVEIFTGDEIVKKIIDGVHAAGARVIASNHDFFKTPAQSDIVYRLRK
                     MQDMGADIPKIAVMPQNKRDVLTLLSATEEMVTDYADRPIITMSMAGTGVISRLCGEV
                     "
     assembly_gap    3064..3152
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3386..4003
                     /locus_tag="DHV81_07970"
     CDS             3386..4003
                     /locus_tag="DHV81_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCI94382.1"
                     /translation="MKKILHYLDEYLEEFLMVIFLIAMTLIMGIQVFSRYILGMSLSW
                     SEEITRYLFIWSAFLSVSLCTRKCISIKIDQFIQLFPRRGKSIWKVLNLTVEFVFFVY
                     LIPYSFIYLKNTIESGQVSPACGIPMYYVQAAPFVCFIITAFRIAQRWFGEWKIVLKK
                     DEKPLEEGSIASVIEEAVVEDTYREKELVEDIEAIHSKAEKRKEK"
     gene            4004..5293
                     /locus_tag="DHV81_07975"
     CDS             4004..5293
                     /locus_tag="DHV81_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HCI94383.1"
                     /translation="MSAAVVFILFVICLVIAIPVSISLGIVAVLPGAFDSSFTASASY
                     VIRSMLGGIDSFPLLAVPMFVLAGIVMAHGKISQKLFDVFVYFIGKRTAGIPCAVIIT
                     CLFYGAISGSAPATVAAVGSMTIPLLMELGYKKDFSTAIVAVAGGLGVIIPPSIPFIM
                     YAMATGESVSDLFLAGIIPGLLIGALLMFYAYYHCKRYGEDKEKINAKVSELRSKGLF
                     SILKDSIWALLSPVIVLGCIYAGIASPTEAAVISVFYALFVSLFIYKSIKVKEIWGIM
                     VEAMKTYAPILFILAASTAFSRVLTLMQVPQTVSAWILAHFTNEIVLLIVINVFLLIV
                     GMVMDTTPAILILSPILLPIVQSIGMNPIHFGVVMIVNLAIGFVTPPIGVNLFVASSL
                     TDVPVMRIAKKAAPMIGYFLLALLLLTFIPVISLCLL"
     gene            5499..6500
                     /locus_tag="DHV81_07980"
     CDS             5499..6500
                     /locus_tag="DHV81_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HCI94384.1"
                     /translation="MMILSLTAGLAACGGSAGEGSQAAAKAAGVQRYAWPLGSSSPED
                     TVTQIYAEKFADEVDRLSDGKMKISVYPNSVLGGDRELLESCKDGDIPFVVQNTAPQV
                     TFMKDTAVFDMPALFDSIDEVREHVDNPRFMKLMQQVYNDGGYELLGYADQGFRVMTT
                     NKKIESLADFKGQKIRTMENSYHMAYWKALGASPTPMTFSEVYIGLQQGTIDAQENPY
                     EVIVSNKLYEQQDYVVETNHLPHLISLIVSEEFMKGLTDEQQQIIREAAETAKQYARE
                     QSDERIASKIKTIEDSGTQIITLSDEVHEQIRKECQPIYESIEKNVSSDIVDAYLTQ"
     gene            6689..7492
                     /locus_tag="DHV81_07985"
     CDS             6689..7492
                     /locus_tag="DHV81_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCI94385.1"
                     /translation="MDKIEEIRAKIGECDDIIIKQLAVRMSHIQEIISYKKATGIPIL
                     QPEQEKKQTDALAAKLGDNEFEEEILDIFKYIMKNSRRIQAKSLFDYNIFLIGFMGAG
                     KSTVAGELKDKLEMDRVEMDQMIVENRGMSISEIFDEFGEAYFRNLESNTLIELQKRK
                     QTIVSCGGGVVMREENADHMKKNGRVVLLTAKPETIYERVKDSDERPILNGNMNVEYI
                     SGLMEKRKERYEAVADVTVATDGKNVTQICEEIIAKLIALDNEQDNKQA"
BASE COUNT         2332 a         1431 c         1773 g         2031 t
ORIGIN      
        1 tataacatga ttagtacagt aatgctatta aaaaataaca aaataagcat tggaagtacc
       61 aaaaattatg cgttacaata tcattatcaa aggttgttaa gtttttaacg aagtgggcat
      121 cgttgaaaac atagctaagt aaaatacgaa taatcaatat tatcacattt aaaaatgaag
      181 gagatttaat catggcagag agaatcacag gacacacaga actcatcgga cttatggctt
      241 acccaatcag acattcaagc tcaccggcaa tgcacaatga ggcatttgca tatcttggac
      301 ttgactacgc ttatctggca tttgaggttg acaacaacac tcttgaggat gcggttaagg
      361 gaatcagagc attaaagctt gtaggctcta atgtatcaat gccaaacaag acagttgtag
      421 gacagtactt agacaagctt tcaccggcag cagagctttg cggagctgta aatacaatag
      481 taaacgagaa cggagtactt acaggacaca tcacagacgg tatcggcttc atgcaggcac
      541 tcaaggataa cgacatcgat gttatcggaa agaagatgac aatcgcagga gcaggcggag
      601 ctgctacagc tatcgagatt caggcagcac ttgacggtgt caaggaaatc tcaatcttca
      661 acatccatga caagttctgg gcaaacgctg aggagacagt tagaaagatc aacgagaaga
      721 cagactgcaa ggcaactctt tacgaccttg atgacaagga gaagttaaga gaagagatgg
      781 ctgattccta catcttcgta aacggaacag gtgttggcat gaagccactc gagggaatgt
      841 ctgtagtacc tgacaaatca ttcttccgtc ctgagcttat cgtaattgac gtaccttact
      901 ctccacttga gacaaagatg cgttctatgg caaaagaggt gggctgcaag acaatgaacg
      961 gtcttggaat gatgcttttc cagggagcag cagccttcaa actctggaca ggaaaagata
     1021 tgccaatcga gcatatgaag gaagtattaa acatcagata tgatgattaa tcagccgtag
     1081 aaattaaatc acattgaatt attttgacag aaattaagga gaacactgat gaataaaaaa
     1141 tatttaccta gcgcattaat cctttactta aactacttta tacacggagt gggctgctct
     1201 atcctcggac aggcagtcat caaggaggct ctcgcagctt catggggagt gcaggttatg
     1261 gctatcacag ctatttcggc agcccttgga cttggacgtc ttatcgcact tccgtttgca
     1321 ggccctttat cagataagct cggacgccgt atctcaacag caatcggaag cggctcttat
     1381 gctatctacc tgatcggact tgctcttgca ttcaatgcag gaacacatgg tggataccag
     1441 attgcttata tctgtgctat cattggcggt attgcaaact cattccttga tacaggaatt
     1501 taccctgcag tatcagaaat catctacaaa gcaccgggtg ttgcgacaat gggaatcaaa
     1561 ttcttcatcg ctatcgcaca gatgctttta ccattcgtac ttggtatgac agttgctaca
     1621 acagcagcag gacttacatc atacaacaca ctgttcttcg gatgtggaat catctacatt
     1681 gtacttttag tattagtaat gatcttcccg cttcctgata gcgatgcaaa ggcagaggga
     1741 aagaaagaag gacttatcgc aagcttaaag cacacacatt tttctgttga gtcaatcgca
     1801 atgattatca ttggatttac atgtacagga accttccagt tatggctcaa ctgtgcacag
     1861 aactttgcaa aagagaacgt aggctgggct gatccgtcag ttatgcagtc atactactca
     1921 gcaggaacca tgatagctct tatcgtaact gcacttttaa caagaaaaat caaggacgtc
     1981 agatttatcc ttgtttaccc tgtaatctgc cttgcaacaa tggtagtagt gttagtcgga
     2041 cagacaaagc cactcatgat cgcaggtgca ttcatcatcg gatgggcagg agcaggcgga
     2101 cttttacaga tcgtaacatc tgtatgcaac atgctcttcc ctaagatcaa gggaacagta
     2161 acagcgcttg taatgatcgc ttcatcactt tgtaactaca caatccttac agcagcatca
     2221 aagatgcagc catcacaggt tatggtaatg aacatcgtgc tcacagcaat cggcgtggta
     2281 cttggattat tcgtaaacct gcgctatact aagatggtag agctgtctaa agaggattaa
     2341 tacgttacat tatactgggg agtataatta tcatacagtc taatacaagg taagaaagga
     2401 aaaaatcatg aacaccatta aggttagaga tattgaaatc ggtgcgggcg cacctaaaat
     2461 catcgttcct atcgtaggcg ttacaaaaga cgacatcatc gcagaggcaa agacatttga
     2521 ctctatccct gttgatatgg ttgagtggcg tgttgactgg ttcgagaatg tattcgagtt
     2581 tgacaaggtt gaggaagtat taaaggagct tcgtgatgct ctcggaaata ttccaatcct
     2641 tatgacattc cgtacatcaa aagagggcgg agagaaagct atcgagcctg aggcttatgc
     2701 aaagcttaat atcaaggctg cacagacagg atacgttgat tttgtagatg tagagatttt
     2761 tacaggcgat gagattgtaa agaaaatcat cgacggcgta catgctgcag gtgcaagagt
     2821 tatcgcttca aaccatgatt tcttcaagac tcctgcacag tctgatattg tttacagact
     2881 tcgcaagatg caggatatgg gagctgatat ccctaagatt gcggttatgc cacagaataa
     2941 gcgagacgta ctcacacttc tttcagctac agaggaaatg gttacagatt acgctgacag
     3001 accaatcatc acaatgtcaa tggcaggcac aggtgttata agccgtctct gcggtgaggt
     3061 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaggatct gtctacggta cttggattac
     3181 ttcataagag catgtaagaa aagcttgtat aaaaccatgc aataaaataa aatggcgcct
     3241 cggcatctgt aaactatgtg ctgtgacgcc atttttgaag atgataacaa atagtaatat
     3301 ataaaattgt gaatatttac gtggaaaaac agcaataatt cagtaaaaat aatacgtata
     3361 attattcacg gaaaggggac atttcatgaa gaaaatactg cactatctgg atgaatatct
     3421 tgaagaattt ttaatggtca ttttcctgat tgcgatgact cttatcatgg gtattcaggt
     3481 gttttcaaga tatattcttg gcatgtcgct ttcatggtct gaggaaatca caagatacct
     3541 gtttatctgg tcggcatttt tgagtgtcag cctttgtaca cgtaaatgca tatccataaa
     3601 gatagaccag tttatacagc tttttccacg aagaggaaaa tcaatctgga aggtattaaa
     3661 tctgacagtt gagtttgtgt ttttcgtata tctgataccg tattcattca tatatctgaa
     3721 aaatacaata gaaagcggtc aggtaagtcc tgcctgcggt atccctatgt actatgtgca
     3781 ggctgcaccg tttgtatgct ttataatcac agccttccgc atcgcgcaga gatggtttgg
     3841 cgagtggaaa attgtcctga aaaaagatga aaagcctctt gaggagggca gtattgcctc
     3901 ggttatagag gaagccgttg tcgaagatac ttaccgcgag aaagagcttg tggaggatat
     3961 tgaagcaatt catagtaaag ctgaaaagag aaaggagaaa tagatgtcag cagcagtagt
     4021 ttttatatta tttgtgatat gtctggtcat tgctattccg gtatctattt ccctgggaat
     4081 agtggcagtg ctgccgggcg cgtttgactc atcttttaca gccagtgcca gctatgttat
     4141 ccgctctatg ctcggtggta tagacagctt cccgctttta gcggtgccta tgtttgtgct
     4201 tgccggaatc gtcatggcac acggcaaaat ctcacaaaaa ttgtttgatg tttttgtgta
     4261 ttttatagga aaaagaaccg caggaatccc atgtgcggtt atcatcacct gcttgttcta
     4321 tggagcgatt tccggctctg cgcctgcaac tgttgcggct gtcggaagta tgaccattcc
     4381 gcttcttatg gagctgggct acaaaaagga tttttcaacg gcaattgttg cggttgcggg
     4441 aggactcgga gtcatcatac cgccaagcat accttttatc atgtatgcga tggcaaccgg
     4501 tgagtcggtc agtgatctct ttcttgcagg aatcattccg ggacttttga taggagcgct
     4561 tcttatgttt tatgcatatt atcactgcaa gcgctatgga gaggacaagg aaaagataaa
     4621 tgcaaaggtc tctgagctta gaagcaaggg tctttttagt atattgaagg acagtatatg
     4681 ggcacttttg agcccggtta tagtacttgg atgcatttac gccggtatag cttcgcctac
     4741 agaggctgcg gtgatttcag tattttatgc gctttttgta agtcttttta tttacaaatc
     4801 cataaaggta aaagagatat ggggcatcat ggtggaggcg atgaagacat acgcacctat
     4861 attgtttata cttgccgcat cgaccgcgtt ttcacgcgtg ctcacactca tgcaggtgcc
     4921 acagacagtc agcgcatgga tactcgcaca ttttacaaat gagatagtgc ttcttattgt
     4981 gataaatgtg ttcctgctca ttgttggaat ggttatggat accacaccgg ctatacttat
     5041 tttaagccca attctgcttc ctatcgtgca gagcatcgga atgaacccga tccactttgg
     5101 tgtggtcatg atcgtaaacc ttgcaatcgg atttgttaca ccgcctatag gagtaaatct
     5161 gtttgtggca agctcactga cggatgttcc ggtcatgcgg atagcaaaga aggcggcgcc
     5221 tatgatagga tatttcctgc tggcacttct gctgcttaca tttattccgg taatcagtct
     5281 gtgcttatta tagagaaata ttaactattc ggtacagaaa tatgtaataa caggtataag
     5341 cagatttaat gatgattgta ggatatttag gcacacagtt gtaattggag aaaaatatga
     5401 gtagatttgg cttgttttta tgtaaaaaag ctggtaaata taataaaccg cgtaaatcgt
     5461 atagcaaata tagattgtgg cgaagagcag ccgccgtcat gatgatactt tcgcttacag
     5521 caggacttgc cgcgtgcggt ggctctgctg gtgaaggctc gcaggctgca gctaaggctg
     5581 ccggggtaca gcgttacgca tggcctctcg gcagttcaag tcctgaggac accgtgacac
     5641 agatatatgc agagaaattt gccgatgaag tggacagatt aagcgatggc aagatgaaaa
     5701 tcagtgtgta tccaaacagt gtactgggcg gagacagaga gcttctcgag tcatgcaagg
     5761 atggagatat accttttgta gtgcagaaca ctgcgcccca ggtaactttc atgaaggata
     5821 ctgcagtatt tgatatgccg gcactctttg acagcattga tgaggtgcgt gagcatgtgg
     5881 acaatccaag gtttatgaag ctcatgcagc aggtctacaa tgacggcggc tacgagcttt
     5941 tgggatatgc agaccaggga ttccgtgtta tgacaacaaa caaaaagata gaaagccttg
     6001 ctgattttaa gggtcagaaa atccgtacaa tggaaaactc ttatcatatg gcatactgga
     6061 aggcgctcgg agcaagcccg acacctatga ctttctcgga ggtgtatatc ggcttacagc
     6121 agggcaccat agatgcgcag gaaaacccat acgaggtaat cgtatccaat aagctctatg
     6181 agcagcagga ttatgtagtg gagacaaatc atctgccaca tctgatatcg ctcatagtaa
     6241 gcgaggagtt catgaaagga ctgacagatg agcagcagca gattatccgt gaggcagccg
     6301 agacagcgaa acagtacgcc agagagcagt ctgacgagcg tattgcctca aagataaaga
     6361 ctatcgagga cagtggcaca caaatcatca ctttaagcga tgaagtgcat gagcagattc
     6421 gcaaggagtg tcagccgata tatgaatcga ttgagaaaaa tgtgtcgtct gatattgtgg
     6481 atgcatatct gacacaataa cactttaaag cattaaaata ttttgcttga taaattggta
     6541 aagtcttgtt atactatgac taagaaattt gtggttatca aaaatgcgaa atactataac
     6601 gtgaagttat aataattata atttgacgat atttagatta aatatcaaaa tccaatatga
     6661 aaaccacaca taagtaagga gacacaccat ggacaaaatc gaggaaatcc gcgcaaagat
     6721 tggagaatgt gacgatataa taattaagca gcttgcggtc agaatgtcac acattcagga
     6781 gattatatca tataagaagg caacaggaat cccaatctta cagccggagc aggagaaaaa
     6841 gcagacagat gcacttgcgg caaagcttgg cgacaatgaa tttgaggaag agatacttga
     6901 tattttcaag tatatcatga agaacagtcg tcgtatccag gcaaagagcc ttttcgatta
     6961 caatatcttc ctgatcggtt tcatgggtgc aggaaaaagc actgtagcag gagaattaaa
     7021 ggataagctc gagatggacc gtgtcgagat ggatcagatg attgttgaga atcgtggcat
     7081 gtcaatctca gaaatatttg atgagttcgg tgaggcatat ttcagaaact tagagagcaa
     7141 cacacttata gagcttcaga agagaaagca gaccatcgtt tcctgcggtg gtggtgttgt
     7201 catgagagag gaaaacgcag atcatatgaa gaaaaacggc cgtgtagttc ttcttacagc
     7261 aaagcctgag actatctacg agcgagtaaa ggatagtgac gagcgcccta tactaaacgg
     7321 caacatgaat gttgagtata tcagtggcct tatggaaaag cgaaaggagc gctatgaggc
     7381 agtggcagat gtaacagttg ctacagatgg caagaatgtt acacagatat gcgaggaaat
     7441 cattgcaaag cttatcgcac ttgataatga gcaggacaat aaacaggcat aatacaggaa
     7501 tagcacagca cttattaagg cagcagccgg atatcagacc ggctgttgct ttttgttcaa
     7561 atactttagg ggtgtgttgc ttgaaataat tgcaaatatg atgtatagta ctctacaatt
     7621 taagagatac tgtttaagta tggcagcata acaagg
//
LOCUS       DPUF01000227            4271 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_20300, whole
            genome shotgun sequence.
ACCESSION   DPUF01000227 DPUF01000000
VERSION     DPUF01000227.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_20300"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="DHV81_07990"
     CDS             <1..1140
                     /locus_tag="DHV81_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCI94386.1"
                     /translation="AKQPFEIQAAEVTVEGASTPDYPLQKKRHTFEYLRTISHLRPRT
                     NTFEAVFRVRSLCAYAIHKFFQERDFVYVHTPLITGSDCEGAGEMFQVTTLDLNNLPM
                     TEEGKVDFSKDFFNKPTNLTVSGQLNGETYAMAFKNIYTFGPTFRAENSNTTRHAAEF
                     WMIEPEIAFADLEDDMMLAESMLKYVISYVLENAPEEMAFFNNFVDKGLLDRLRNVVE
                     NDFARVTYTEAIDILSKHNDKFDYKVSWGCDLQTEHERYLTEQIYKRPVFVTDYPKEI
                     KAFYMKLNDDGKTVAAVDCLVPGIGEIIGGSQREDDYDKLLARINELGLSEDDYKFYL
                     DLRKYGSARHAGFGLGFERCVMYLTGMGNIRDVIPFPRTVNNCEL"
     gene            1212..1553
                     /locus_tag="DHV81_07995"
     CDS             1212..1553
                     /locus_tag="DHV81_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94387.1"
                     /translation="MWRKIKGPVITICIMHLTALLCLIILSIVQCFVDMAEWIKFALA
                     MAVNIVVGLVCGFLLKCCFHRRRFIRVTVVAYWITYVIATVVIDIGESKNIFELLIIL
                     LLVMFSALVFR"
     assembly_gap    1691..1867
                     /estimated_length=177
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1903..3273
                     /gene="scfB"
                     /locus_tag="DHV81_08000"
     CDS             1903..3273
                     /gene="scfB"
                     /locus_tag="DHV81_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HCI94388.1"
                     /translation="MVHQYKNNGYNIVLDVNSGAIHVVDDVTYDVIELFEDNSAKEII
                     EKLVDRYPQTEIEEAIQEVNELKDNEELFTEDTYKERIIDFKKRQTVVKALCLHIAHD
                     CNLACRYCFAEEGEYHGRRALMSYETGKQALDFLIANSGSRRNLEVDFFGGEPLMNWD
                     VVKQLVAYGREQEKLHDKHFRFTLTTNGVLLNDEIMEFANKEMDNVVLSIDGRKEVHD
                     RMRPFRKGAGSYDLIVPKFQKFAESRHQDKYYVRGTYTHFNTDFSKDVLHLADLGFKQ
                     ISVEPVVAQPTDEYALKESDLPVLFDEYDRLAAEMVKRNKAGNGFNFFHFMIDLEGGP
                     CVYKRLSGCGSGTEYLAVTPWGDLYPCHQFVGNEDFLMGNVWDGIKATDIQDEFKCCN
                     VYAKEKCRNCFARFYCSGGCAANSYNFHGTITDAYDLGCELQKKRIECAIMIKAAEAD
                     MADDAQ"
     gene            3364..>4271
                     /locus_tag="DHV81_08005"
     CDS             3364..>4271
                     /locus_tag="DHV81_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCI94389.1"
                     /translation="MTGRYKKMKKGKSWVIILVAVICIGLLGWYSTAVITDTKQKQEA
                     TANGESTKDKDYQNIKLGLDLSGGVSITYHIKDKNPSDKDVNDTIAKLEERAESYTTE
                     YSVYKSGDDRITVEIPGVDDPNKVLEDLGSPGSLYFIAQYDSDGNANYTQDTSVEGSY
                     KLNYDIDTLKANGSVILDGNDVKDASAQYDQSSSTSTKEPVVSLKLKDKAIDTWADAT
                     KKAKDAGQSIGIYYDDHFISVPTVSAVISDGNCVINGMKDYDEATSLATFIRVGAINL
                     QLEELESNVVGAQLGGTALTNAVKAA"
BASE COUNT         1239 a          779 c         1013 g         1063 t
ORIGIN      
        1 gcaaagcagc catttgagat tcaagccgca gaggtgactg ttgagggtgc atccacaccg
       61 gattatcctc tgcagaagaa gagacataca tttgagtatc tccgtacaat ctcacactta
      121 agacctcgta caaatacttt tgaggcagtt ttcagggtgc gctcactctg cgcatatgct
      181 attcacaaat tcttccagga gagggatttt gtgtacgtgc acacaccgct tatcacagga
      241 agtgactgtg agggtgcagg agagatgttc caggtgacca cacttgattt gaacaatctc
      301 cctatgacag aggaaggaaa ggtggatttc tcaaaggatt tctttaataa gcctaccaac
      361 cttactgttt caggacagtt aaacggcgag acatatgcta tggcattcaa gaatatctac
      421 accttcggac ctaccttccg tgcggagaat tcaaacacca cacgtcatgc ggcagagttc
      481 tggatgattg agcctgagat tgcttttgca gaccttgagg atgatatgat gcttgcagag
      541 agcatgctca agtatgttat cagctatgtg cttgaaaatg cacctgagga gatggcattt
      601 ttcaacaatt tcgtggacaa gggactcctt gacagattaa gaaatgttgt tgaaaatgat
      661 tttgcccgtg tgacatacac agaggctatc gacatccttt caaagcacaa tgacaagttt
      721 gattacaagg tatcatgggg ctgtgattta cagacagagc atgagagata ccttacagag
      781 cagatttaca agcgccctgt atttgtcaca gattatccga aggagataaa ggccttctac
      841 atgaagctga atgacgacgg aaagaccgtt gcagcagttg actgtcttgt accgggaatc
      901 ggagaaatca tcggtggaag ccagagagag gatgactacg ataagcttct tgccagaata
      961 aatgagcttg gactttcaga ggatgactac aagttctacc ttgacttaag aaaatacggc
     1021 tctgcaagac acgcaggctt tggactgggc tttgagagat gcgtaatgta tcttaccggc
     1081 atgggcaata tccgtgacgt tatcccattc cctagaacag ttaataactg tgagctgtaa
     1141 tcacgaatga atttcatata tgaataatta ccgccctttg cacatatata tgatgtgtgg
     1201 agggcgtttt tatgtggaga aaaatcaaag gtcctgtgat aacaatatgt attatgcatc
     1261 ttacggcgct tttgtgcctt atcatacttt ctatagtaca gtgctttgtc gacatggcgg
     1321 agtggataaa gtttgcactt gccatggcgg tcaatatagt ggtggggctt gtgtgcggat
     1381 ttttgctcaa atgctgcttt caccgcagac gttttatcag ggtgacggtc gtagcatact
     1441 ggataacgta tgtgatagca acagtggtca tagatatagg tgaatccaaa aatattttcg
     1501 agcttttgat aatactcctt ctggtaatgt tttcagcgct tgtttttcgc taaaaaagga
     1561 cttttcatat tgataatgct gtgatataat gttgaaagca aatatgatgt gtcaatgaga
     1621 catcgttaaa tgaaaaggag ataaaaatga aacacgtaaa gacattaaat acaagaaaat
     1681 tacagaacac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnttt taaaatgtgt atagaaagag aggaatattg tagtggttca tcagtacaag
     1921 aacaacggct ataatattgt cttagatgtc aacagtggcg caatccatgt ggtggatgat
     1981 gtcacatatg acgttattga gctgtttgag gacaactcag ctaaggagat tattgaaaag
     2041 cttgtggaca ggtatccaca gacggagata gaagaagcaa tacaggaagt aaatgagctt
     2101 aaggataatg aagagctctt cacagaagat acatacaaag agcgtatcat agatttcaag
     2161 aagcgccaga cagttgtaaa ggcactttgc cttcacatcg cgcacgactg caaccttgcc
     2221 tgcagatatt gttttgcaga ggagggcgag tatcacggca gacgagctct catgagctac
     2281 gagacaggaa aacaggcact tgatttcctg attgccaatt caggcagcag aagaaactta
     2341 gaggtagact tctttggagg agagcctctc atgaactggg atgtggtgaa gcagcttgtg
     2401 gcatatggaa gagagcagga gaagcttcat gacaagcatt tcaggttcac actcaccaca
     2461 aacggtgtgc tcttaaatga cgagattatg gagtttgcca acaaagagat ggataatgta
     2521 gttcttagta tagacggacg caaggaggtt catgaccgta tgagaccttt cagaaagggt
     2581 gccggcagtt atgaccttat tgttccgaaa ttccagaagt ttgcagagag cagacatcag
     2641 gacaagtact atgtgagagg tacatacaca catttcaata ctgatttttc aaaggatgtg
     2701 cttcatctgg ctgatttagg cttcaaacag atttcggttg agcctgtggt ggcacagcct
     2761 acagatgagt atgcacttaa ggaatctgat ttacctgtgc tctttgatga gtacgacaga
     2821 cttgcagcag agatggtaaa gagaaacaag gcaggaaacg gcttcaattt cttccatttt
     2881 atgatagatt tagagggagg accttgtgta tataagcgtc tttccggttg tggctcagga
     2941 actgagtacc tggcagtgac tccatgggga gatttatatc catgccacca gtttgtgggc
     3001 aatgaggact tccttatggg taatgtctgg gatggcatca aggccactga catccaggat
     3061 gagtttaagt gctgtaatgt ttatgccaag gaaaaatgtc gcaactgttt tgccagattc
     3121 tattgcagcg gtggctgtgc cgccaactca tacaacttcc acggcacaat cactgatgcc
     3181 tatgatttag gctgcgagct gcagaagaag cgtatagagt gcgctatcat gattaaggca
     3241 gcagaggctg atatggcaga tgatgcacaa taatttgaat ttgtcacggg aaacggatag
     3301 cggtatttgt gacccgtgtg cgaatatatt tgccatctgt atacctatat ggatggtagc
     3361 aaaatgacag gaaggtataa gaaaatgaaa aaaggaaaga gttgggtgat tatccttgtg
     3421 gcagtcattt gtatcggctt gctgggctgg tattccactg cagtaatcac cgacacaaag
     3481 caaaagcagg aggcaactgc taatggtgag agcaccaagg ataaggatta tcagaatatc
     3541 aagcttggtc tcgacctctc aggtggtgtt tcaatcacct accatatcaa ggacaagaat
     3601 ccttcagaca aggatgtaaa tgatactatc gcaaagctcg aggagcgtgc agagagctac
     3661 acaacagagt actctgtata caagagcggg gatgaccgta tcactgtaga gattccgggt
     3721 gttgacgacc ctaacaaggt actcgaggat cttggaagtc cgggatcact gtacttcatc
     3781 gcacagtatg acagtgacgg aaatgccaac tatacacagg acacatcggt tgagggaagc
     3841 tacaagctca actatgatat tgacacatta aaggccaatg gttcagtcat cctcgatggt
     3901 aacgatgtaa aggatgcaag cgcccagtat gaccagtcat catctacatc aaccaaggag
     3961 cctgttgttt cattaaagct caaggataag gctattgaca catgggcaga tgccacaaag
     4021 aaggcaaagg atgcaggaca gtcaatcggt atatactatg atgatcattt catcagcgtt
     4081 cctacagtaa gcgccgttat ctcagatggt aactgtgtaa tcaacggaat gaaggattac
     4141 gacgaggcaa caagccttgc aacatttatc cgtgtcggag ccatcaacct tcagctcgag
     4201 gagttagagt caaatgttgt tggcgcacag ctcggtggaa cagcgctcac aaatgcagta
     4261 aaggctgcaa t
//
LOCUS       DPUF01000228            6118 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23472, whole
            genome shotgun sequence.
ACCESSION   DPUF01000228 DPUF01000000
VERSION     DPUF01000228.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23472"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="DHV81_08010"
     CDS             <1..855
                     /locus_tag="DHV81_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCI94390.1"
                     /translation="DSLFSGFSHIDDDFYEELEEILIMGDLGVRATESIIEDLQRKVK
                     EQHIKEPAECKELLIESIKEQMKVEETAYRFENEKSVVLVIGVNGVGKTTTVGKLAGK
                     LKEQGKKVVIAGADTFRAAAGDQLKEWANRAGVDMIGGAEGADPGAVVYDAVAASKAR
                     NADVLLVDTAGRLHNKKNLMNELGKINKILESEYPDAYRETLVVLDATTGQNALVQAR
                     EFSDVAKISGIVLTKMDGTAKGGIAVAIQSELSVPVKYIGVGETIDDLQKFDSDEFVN
                     ALFDTKTE"
     gene            1053..>1999
                     /locus_tag="DHV81_08015"
     CDS             1053..>1999
                     /locus_tag="DHV81_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HCI94391.1"
                     /translation="MLTLDKFEEASEKVKEVTLETKLIYSDFLSDQTGNKVYLKPENM
                     QFTGAYKVRGAYYKISTLTDEERAKGLITASAGNHAQGVAYAAKCYGCKAVIVMPTTT
                     PLIKVNRTKSYGAEVVLYGDVYDEACAKAYELAEKEGYTFIHPFDDLAVATGQGTIAM
                     EIFKELPLVEYILVPIGGGGLATGVSTLAKLLNPKIKVIGVEPAGANCMQESFKNGKV
                     TTLSSVNTIADGTAVKTPGSNIFPYIKKNLDDIITVEDDELIVAFLDMVENHKMIVEN
                     SGLLTVAALKHLGVKDKRVVSILSGGNMDVITMSSVVQQG"
     assembly_gap    2000..2125
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2126..2281
                     /locus_tag="DHV81_08020"
     CDS             <2126..2281
                     /locus_tag="DHV81_08020"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881984.1"
                     /note="catalyzes the formation of 2-oxobutanoate from
                     L-threonine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HCI94392.1"
                     /translation="KLEHNQFVTTNRSAAVELRITLECFGTEHKEQIIAALEKKGYKP
                     RIVRTMI"
     gene            2370..2714
                     /locus_tag="DHV81_08025"
     CDS             2370..2714
                     /locus_tag="DHV81_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCI94393.1"
                     /translation="MEEKLEQAYLYDFYGELLNEHQRQVYEDFVFNDLSLGEIASEEG
                     ISRQGVADLIKRCNKKLLDYEAKLHLVEKFMSIKSDIRRIHELTNDFKKSHNELLMNE
                     IEAISNQILEEL"
     gene            2716..4110
                     /locus_tag="DHV81_08030"
                     /pseudo
     CDS             2716..4110
                     /locus_tag="DHV81_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742358.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
     assembly_gap    3531..3624
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4221..4466
                     /locus_tag="DHV81_08035"
     CDS             4221..4466
                     /locus_tag="DHV81_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCI94394.1"
                     /translation="MAVKIRLRRMGQKKAPFYRIVVADSRSPRDGRCIEEIGTYDPTK
                     DPSEYHVNEELAKKWLSNGAQPTETVARIFKSAGIEK"
     gene            4484..4714
                     /locus_tag="DHV81_08040"
     CDS             4484..4714
                     /locus_tag="DHV81_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCI94395.1"
                     /translation="MKELVEVIAKSLVDYPDEVNVTETENEKAIVLELRVAQSDMGKV
                     IGKQGRIAKAIRSVVKAAASKEDKKVIVEIVQ"
     gene            4847..5353
                     /locus_tag="DHV81_08045"
     CDS             4847..5353
                     /locus_tag="DHV81_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HCI94396.1"
                     /translation="MEDLLQVGVITSTHGIRGEVKVFPTTDDAARFKKLKNVILDNGK
                     ERRDLEITSVKFFKNQVILKFKGIDDINDVEKYKKAPLFVTRENAVPLEENEYFIADL
                     IGLSVTSDEGEELGVIDDVLQTGANDVYVVKKAGANDLLIPAIKDCILDVDVENGTMK
                     VHLLAGLR"
     gene            5460..>6118
                     /locus_tag="DHV81_08050"
     CDS             5460..>6118
                     /locus_tag="DHV81_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCI94397.1"
                     /translation="MRFHVLTLFPQMIEQGLSESITGRALKQNIISLNTVNIRDFAHN
                     KHNKVDDYTYGGGAGMLMQAEPVYQAVRSVVSQINKCNQVHSGDNSEKNIADENILYE
                     NTSYKNTAEEIKNHNARLIYVTPQGSVFNQQMAAEFAKCDDLIFLCGHYEGIDERVLE
                     ETVTDYVSIGDYVLTGGELPSMVMIDAISRLVPGVLHNDISAETESFHGNLLEYPQYS
                     RP"
BASE COUNT         1865 a          992 c         1554 g         1487 t
ORIGIN      
        1 gattcacttt tcagtggatt ttcacatata gatgatgatt tttatgagga gcttgaggag
       61 attctcatca tgggagacct cggagtgaga gctacagaga gtatcatcga ggatctgcag
      121 aggaaggtga aggagcagca catcaaggag ccggcagagt gcaaggaact cttgattgag
      181 agcatcaagg agcagatgaa agtggaggag acagcctaca gatttgaaaa tgaaaaatct
      241 gttgtgcttg taatcggtgt caacggagta ggaaagacta ccactgtcgg aaagcttgca
      301 ggaaagctca aggaacaggg caagaaggta gttattgcag gggccgatac cttcagggcg
      361 gcagcaggag accagctgaa ggaatgggca aaccgcgccg gggttgatat gattggcggt
      421 gccgagggtg ctgacccggg agctgttgta tatgatgcag ttgcagcttc aaaggccaga
      481 aacgcagatg tgcttctcgt ggatacagcc ggaagactcc acaacaaaaa gaatctgatg
      541 aatgagctcg gaaagattaa taaaatcctg gagagtgagt atccggatgc gtacagagag
      601 acgctcgtcg tgcttgatgc gaccacaggc cagaatgcac tggttcaggc gagagagttt
      661 tctgatgtgg caaagatttc gggcatcgtg cttactaaga tggatggtac ggcaaagggc
      721 ggtatcgctg tagcgataca gtcggagctt tcagtgccgg tcaagtatat cggagtcggt
      781 gagactatcg atgatttgca gaaatttgat tcggacgagt tcgtgaatgc attgtttgat
      841 actaaaacgg aataatttga gaatatcttt gagcgtataa gttatattgt ttataataat
      901 ctgatatatc agtttatata tatctgtacg gaatttttgg tataatataa tgaaaaataa
      961 ctattcagaa ccactgagaa caatgcattt ttgcgaataa tgtgtgggtg ggtatgaata
     1021 gttataccga agaaatgaaa gggagaacaa atatgctgac attagataaa tttgaagaag
     1081 caagtgagaa ggtcaaggag gtcacccttg agacaaagct catttacagt gactttttaa
     1141 gtgaccagac aggcaataag gtatatctga agcctgaaaa tatgcagttt accggagctt
     1201 acaaggtcag aggtgcgtac tacaagataa gcacactcac cgatgaggag agggcaaagg
     1261 gactcatcac agcatctgca ggaaaccatg cgcagggtgt tgcgtatgct gcaaagtgct
     1321 atggctgcaa ggctgtaatc gtgatgccta ccaccacacc tcttatcaag gtaaaccgta
     1381 caaagagcta cggcgctgag gtagtgcttt atggagatgt gtatgatgag gcatgtgcca
     1441 aggcatatga gcttgcagag aaagagggct acacatttat ccatccgttt gatgatttag
     1501 ctgtcgcaac aggacaggga actattgcta tggagatatt taaggagctt cctcttgttg
     1561 agtacatcct cgttccaatc ggcggcggcg ggctggcaac aggtgtatcc acacttgcaa
     1621 agctcttaaa cccaaagatt aaggttatcg gagtggagcc ggccggtgcc aattgtatgc
     1681 aggagtcatt taagaacggc aaggtgacca ctttatcaag tgtaaatact attgcagacg
     1741 gaacagcggt aaagacaccg ggctcaaata ttttcccata cataaagaaa aatcttgatg
     1801 atattatcac agtggaggat gacgagctta tcgtggcctt ccttgacatg gttgagaatc
     1861 acaagatgat agtcgaaaac tcaggtcttc tgactgtagc tgcgcttaag catctcggag
     1921 taaaggacaa gagagtggtt tctatcctaa gtggcggcaa catggatgtt attaccatgt
     1981 catcagttgt acagcagggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnaagct ggagcataac cagtttgtca ctacaaacag
     2161 aagtgcggct gtcgagcttc gcatcacgct tgagtgtttt ggtacagagc acaaggagca
     2221 gattattgct gcgctcgaga agaagggcta taagccaaga attgtacgca ctatgatata
     2281 aaaataaaag tatcaagtat ttttacttga caccgggcaa actatgtgtt attctaccat
     2341 aggttcgagg gaaaaagagg atacttgata tggaagaaaa acttgaacag gcatatttat
     2401 atgatttcta cggtgagctt ttgaatgagc atcagcgtca ggtatatgag gattttgtct
     2461 tcaatgattt gtcactcggt gagattgcat ccgaggaggg gatttcccgt cagggtgttg
     2521 ccgatctgat taagagatgc aataagaagc tgcttgacta tgaggcaaag ttgcatcttg
     2581 ttgaaaagtt catgtccata aagagtgaca tacgaaggat tcatgagctg acaaatgatt
     2641 ttaagaaatc acataatgag ctattaatga atgaaatcga agctatttcg aatcagatac
     2701 tagaggagct gtaatatggc atttgacagt ttatcagaaa aactgcagaa cgtttttaag
     2761 aatctccgca gcaagggaaa gcttactgag gatgatgtaa aggctgctct taaagaggtt
     2821 aagatggcac tccttgaggc cgatgttaac ttcaaggttg ttaagcagtt cacaaagagt
     2881 gttcaggaga gagcaatcgg acaggatgtc atgaatggtc tcaatcccgg ccagatggtc
     2941 atcaaaatcg taaacgaaga gatgattaag cttatgggct ctgagacaac agagattgct
     3001 ttcaggccgg gcaaggagct taccgttatc atgatggtag gtcttcaggg agccggtaag
     3061 acaaccacca cagcaaaaat tgccggaaag cttaagagca agggcaagaa atcactgctt
     3121 gtagcctgtg atgtatatcg tccggctgcg attgagcagt taaagataaa tggtgagaag
     3181 cagggcgttg aggttttctc aatgggtgac aaaaacaagc ctgccgacat cgcaaaggct
     3241 gctgtggagc acgctaagaa aaacgatttc aatgttgtta tcatcgatac tgccggacgt
     3301 cttcatattg atgaggatat gatggcagag cttattgaga taaaagaagc tactgatgtt
     3361 ttccagacca tcctcgtggt agatgccatg acaggtcagg atgcggtaaa tgtagcaggt
     3421 acctttgatg aaaagatagg aattgacgga gttgtcatca caaagcttga tggtgacacc
     3481 agaggtggag cggcactttc aatcagagcc gttacaggaa agccgatact nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnatgggc gatgtgcttt ccatgattga gaaggcgcag
     3661 gagaacattg atgaggaaca ggccaagaag cttgagcaga agatgcgcaa aaatgagttt
     3721 gattttgaga tgtaccttga ttcgatgagt cagatgaaga agatgggcgg tctttcaagc
     3781 gtgatgggca tgatgccggg catgggtatc ggtggaggca agatgccgga tattgataca
     3841 gatgaagctg aaaagggcat ggcccgcaca gaggcaatca tctattccat gacacctgag
     3901 gaaagacgaa atccaaagct tcttaatcca agccgcaagg ctcgtatagc aagaggagca
     3961 ggtgtggatg tatcagaggt caacagactt gtaaagcagt ttgagcagac aaagaagttc
     4021 atgaagcaga tgccaggtat gatgggcgga aagaagggac gccgtggagg cggtggtctt
     4081 ggaggcttat tcaaaggact tccgttttaa tggatttcat taaaggaatt tcattaaaga
     4141 atttcatgtt ttgttaacaa aagtatttat tttacttttg atatatatta ttcaataaac
     4201 ctatgaggag gtgaaacaaa atggcagtaa agatcagatt aagaagaatg ggacagaaga
     4261 aagctccttt ctatagaatc gtagttgcag actctagatc accaagagat ggaagatgca
     4321 tcgaggagat cggaacatac gatccaacta aggatcctag cgagtatcat gtaaatgagg
     4381 aattagctaa gaaatggtta tcaaacggcg cacagccaac tgagacagta gctagaatct
     4441 tcaagagtgc tggtatcgag aaataggata aagaggtggc gtaatgaaag aattagttga
     4501 agtaattgca aaatctttag ttgattatcc ggatgaggtt aatgtaactg agaccgaaaa
     4561 cgagaaagca attgttttgg aattacgtgt tgcacagtct gacatgggca aagtgatcgg
     4621 caagcaggga cgtattgcta aggctattcg ttcagttgtt aaggccgcag cctctaaaga
     4681 agacaaaaag gttattgttg agatagtaca gtaacataaa ggcatctctt atgagatgcc
     4741 tgttttttta taatttatag taactattca gaaccggtat tgtatggttt tgagaatgtg
     4801 cgtgggctga atagttatga tttatattaa tatcaggaga cagagaatgg aagatttact
     4861 acaggtaggt gtaattacga gcacacacgg cattcgcggt gaggtaaagg tgttcccaac
     4921 cactgacgat gctgcccgtt ttaaaaagct taaaaatgtc attctggata atggcaaaga
     4981 gcgcagggat ttggaaataa cttctgtcaa atttttcaag aaccaggtta tcttgaaatt
     5041 taagggcata gatgacataa atgatgtaga aaaatataaa aaggcacctc tttttgtgac
     5101 aagggaaaat gcggtgccac ttgaggaaaa tgagtacttt atagctgatc ttatcgggct
     5161 ttccgtcaca tcagacgaag gagaggagct tggagttata gatgatgtgc tccagaccgg
     5221 tgccaacgat gtgtatgttg ttaagaaagc cggtgcaaat gatctgctga ttccggcaat
     5281 aaaggattgt atactggacg ttgatgtgga aaatggcacg atgaaggtgc atctgctggc
     5341 aggtttgaga tagctacatc tcaaactgca aaaaccgtaa aatgagatat aaaattgaac
     5401 aaaatcatac attttaaatc gtaaaactgt aaaacaggat gatataggag atataggaaa
     5461 tgagatttca tgtgctgacc ttattcccac agatgataga acagggactg tcagagagta
     5521 tcacaggcag agctttaaaa cagaatatta tatcactcaa tacggtaaat atcagggatt
     5581 ttgcgcacaa taagcacaat aaggttgatg attacacata tggtggcggc gcggggatgc
     5641 ttatgcaggc agagccggtg tatcaggctg tgcgcagtgt ggtgagtcag atcaataaat
     5701 gtaatcaggt ccattcaggt gataattcag agaaaaatat cgccgatgaa aatatattat
     5761 atgaaaatac atcatataaa aatactgcag aagaaataaa aaatcacaat gcgcgtctga
     5821 tttatgttac tccacagggc agcgtgttca accagcagat ggcggcagaa tttgcaaagt
     5881 gtgatgacct catttttcta tgtgggcact atgaaggtat tgacgagcgc gtgctggagg
     5941 agacggtaac ggattatgtc tctatcggtg actatgttct aacaggcgga gaactgccgt
     6001 cgatggtaat gatagatgca atttcacgtc tggttcctgg ggtgctgcac aatgacattt
     6061 cggcagagac agagtcgttt catggcaatc tgcttgaata ccctcagtat tcacgtcc
//
LOCUS       DPUF01000229            2786 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16888, whole
            genome shotgun sequence.
ACCESSION   DPUF01000229 DPUF01000000
VERSION     DPUF01000229.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16888"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="DHV81_08055"
     CDS             complement(<1..104)
                     /locus_tag="DHV81_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCI94398.1"
                     /translation="MLADIIVIAILLVIVALVIRTIIKSKKAGKSLQCG"
     gene            complement(277..2427)
                     /gene="feoB"
                     /locus_tag="DHV81_08060"
     CDS             complement(277..2427)
                     /gene="feoB"
                     /locus_tag="DHV81_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCI94399.1"
                     /translation="MTVKIALAGNPNCGKTTLFNALTGANQYVGNWPGVTVEKKEGKL
                     IGHKDVDLVDLPGIYSLSPYTLEEVVARNYLIGEQPDAIINIVDGTNIERNLYLTTQI
                     IELGIPVLMAVNMMDIVKKNGDTINIKALGEKLGCEVVEISALKKTGIKETAEKAIQI
                     AKSGKSVQRVHHFADDVESTIAGIEDKLGLAVSENQKRFFAIKLLERDSKISEVLKSA
                     PNVDAEIKALEDKYDDDTESIITNERYQYISSIIGSCVKKARAGKETVSDKIDKIVTN
                     RFLALPIFALIMWAVYYVAVSSLGGIVTDWTNDTLFGEWIQPAVQTFMENVGCSEWLV
                     SLVVEGIIGGLGAPIGFAPQMAIVFLFLSILEDCGYMARVAFIMDRIFRRFGLSGKSF
                     IPFLIGSGCGVPGIMATRTIENEKDRRMTMMTVTNIPCGAKLPVIALIAGFIMGDGCW
                     WMAPLMYFAGIGLTIIYCIILKKTRAFAGEPAPFVMELPQYHIPSVKGVLLHVWERVW
                     AFLKKAGTILFLCCAVMWFLSSFGIQDGAFGLVDTENSLLAVIGSAIAVIFAPLGFDT
                     WQAVASSLSGFVAKEGIVSTMGVLSGLGEVEEYAVSMHDQFAAFFPTTMVAVSFLLFN
                     LFDSPCLAAISTTAKELNNRKFFWFTIIFQNVSAYCVTLMFYQIVGLCIGEVAFNFWT
                     VVAFVLLAGVLYLLFRKDPNKATVKSTSFAASNV"
     gene            complement(2500..2721)
                     /locus_tag="DHV81_08065"
     CDS             complement(2500..2721)
                     /locus_tag="DHV81_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCI94400.1"
                     /translation="MKTLKQAKVGETVKVVKLTGQGAVKRRIMDMGITKGVEIFIRKV
                     APLGDPVEVTVRGYELSLRKDDASMIEVE"
BASE COUNT          745 a          653 c          532 g          856 t
ORIGIN      
        1 ccacactgca ggctcttgcc tgctttcttt gatttgatta ttgtcctgat tacaagtgca
       61 actatcacta agagaattgc aattacaatg atatcagcca gcatgtcatc atctcctatt
      121 cactacaaat gctactaaat atactactgc attatttctt ttccttgaaa aatcctatta
      181 ttatctttac ggcaagcccc agcaggcata gaagaactat gactgctatg aggtctgctg
      241 tgttaaattc cagattgatt ctcatttaaa catctattac acgtttgatg ccgcaaatga
      301 agtgctcttt actgttgcct tattagggtc ttttctgaac aataagtata atacacctgc
      361 aaggagtaca aatgcaacta ctgtccagaa gttgaaggct acctcaccta tgcaaagtcc
      421 cacaatctgg tagaacataa gtgttacaca gtaagctgat acgttttgga atatgattgt
      481 aaaccagaag aacttacggt tgttaagctc tttagcggtt gttgagatag ctgcaagaca
      541 tggtgagtca aacaggttga acagcaagaa tgatactgct accattgttg ttgggaagaa
      601 tgctgcaaac tgatcatgca tgcttactgc atactcttct acctctccaa gtcctgagag
      661 tacacccatt gttgaaacga taccctcttt tgctacaaag cctgagagtg atgatgctac
      721 tgcctgccat gtatcgaatc caagtggtgc aaagataact gcgattgcag atccgattac
      781 tgcaagtaag ctgttttctg tgtcaacaag tccgaatgcg ccatcctgaa tgccgaagct
      841 tgaaaggaac cacataactg cacagcagag gaagaggatt gttccggcct tcttgaggaa
      901 tgcccatact ctctcccata cgtgtaacag cacacccttt actgaaggga tatgatactg
      961 tgggagctcc attacgaacg gtgcaggctc tcctgcaaat gctcttgtct tctttaaaat
     1021 gatacagtag atgattgtga ggccgattcc tgcaaagtac ataagaggtg ccatccacca
     1081 gcagccgtca cccatgataa agcctgcgat aagtgcgata acaggaagct ttgctccaca
     1141 tgggatattt gttactgtca tcattgtcat acgacggtct ttttcgttct cgatagtacg
     1201 tgttgccatg attccaggta caccacatcc tgatccgatg aggaatggga tgaaggactt
     1261 tcctgagaga ccgaatctac ggaagattct gtccatgatg aaggctactc gtgccatgta
     1321 tccgcagtcc tcaaggattg agaggaataa gaatacgata gccatctgtg gtgcaaatcc
     1381 gataggtgca ccaagacctc cgatgatacc ttcaactaca agtgatacaa gccattcaga
     1441 gcagcctaca ttttccataa atgtctgtac tgccggctga atccactcac caaagagtgt
     1501 gtcgtttgtc cagtctgtta caattccacc aagtgatgat actgcaacat aatatacagc
     1561 ccacatgatg agtgcgaaaa taggcagtgc caaaaatctg tttgtaacaa tcttatcaat
     1621 tttgtctgat actgtctctt ttccggcgcg tgccttcttt acacatgagc cgatgattga
     1681 agatatgtac tgatatctct cgtttgtgat gatactctct gtatcatcat cgtatttgtc
     1741 ctcgagagct ttaatctctg catctacgtt tggagcactc tttagcacct cagaaatctt
     1801 tgagtcacgc tctaaaagct tgattgcaaa gaatctcttc tggttctctg atactgcaag
     1861 accaagctta tcctcgatgc ctgcgattgt actctccaca tcatctgcga agtgatgtac
     1921 cctctgaacg gatttacctg actttgcaat ctggattgcc ttctctgcag tctccttgat
     1981 tcctgtcttc tttaaagctg aaatctcaac aacctcacag ccgagcttct ctccaagtgc
     2041 tttgatattg attgtgtctc cgtttttctt tacaatgtcc atcatattga cagccataag
     2101 caccggaatg ccaagctcga taatctgtgt tgtcagatac agattacgct cgatatttgt
     2161 tccgtccacg atattgataa tcgcatcagg ctgctctccg attaagtaat ttcttgcaac
     2221 aacctcctcg agagtatatg gagaaagtga gtagataccc ggtaaatcca ccaggtctac
     2281 atccttgtga ccgataagct ttccctcttt tttctcaact gtaacaccag gccagttacc
     2341 aacatactga ttggcacctg ttaaggcatt gaacagtgtt gtttttccgc agttcgggtt
     2401 tcctgcgaga gcaattttta ctgtcatgtt ttaatctcct tatttttgtt tgctcattct
     2461 gcctatagtt tgtatttact aacctatagt caaaaaaatt tactctactt ctatcataga
     2521 ggcatcgtcc ttacgaagtg atagctcata gcctctcact gtcacctcta ccggatcgcc
     2581 aagcggtgcc accttgcgga taaatatctc aacaccctta gtaattccca tatccatgat
     2641 acgtctcttg actgcgccct ggcctgtgag cttgacaacc ttgactgtct cgcctacttt
     2701 ggcttgcttt aatgtcttca tgctgactac tcctttttta attttattgt tgtttaaaaa
     2761 atttatataa acgtttgcat aatatc
//
LOCUS       DPUF01000230            5195 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3846, whole
            genome shotgun sequence.
ACCESSION   DPUF01000230 DPUF01000000
VERSION     DPUF01000230.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3846"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="DHV81_08070"
     CDS             complement(<1..842)
                     /locus_tag="DHV81_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI94401.1"
                     /translation="MAKEYFADNARFADLCNNILYGGREVILPENLKERDTTEVLTAL
                     GLDKKTIAMQKMRDIFKNASIKYTGKSYVVLIGVENQSDIHYAIPVKNMFYDVMAYGN
                     QVKETAKKHRKEKDTATSDEFLSGFTKEDKLIPVITITVYLGTKEWDGPRKLSDMFGD
                     VDEELLPFIPDYRINLLAPREITDFTGFRTSIRQLFEVLQNAYDKEKMQEVLHNDDKF
                     SSVDRETVEAINLFAGTDIDIDEKEEVIDMCKAWEDQKNEGRELGERQKIISLIVKKL
                     QKDK"
     assembly_gap    1452..1488
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1712..2071)
                     /locus_tag="DHV81_08075"
     CDS             complement(1712..2071)
                     /locus_tag="DHV81_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94402.1"
                     /translation="MLEELFSKSEFIEKKKILEEDYGLKMSMELEGRMSEMCNVSDYW
                     EEVATEEGKEIGEKQKIISLVVKKLQKDKSVAEIADDLEEKEEVIAPIYEAALSMKPD
                     YDVEKIYELLEKNKKLA"
     gene            complement(3378..4592)
                     /locus_tag="DHV81_08080"
     CDS             complement(3378..4592)
                     /locus_tag="DHV81_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCI94403.1"
                     /translation="MADWTYQIIDQSGKERKGNIVAESEAEAKNKLKMDGNMVISLTK
                     ATALTKEISFSVGGKVKPRDLSVFCRQFTSMVNAGVTILDTLDMLSDQTENKVMAKAI
                     RGVHAEIQKGETLSDGLKKYPNVFPDIMVSMVAAGEASGKIDVAFDRMSAHFEKSAKL
                     NGMIKKAAVYPIIVVIVAIAVVIVMLVKVIPSYSEMFNDLGTELPGITKAVVAMSDFV
                     TGYWYIVIAVIAAIVIAIKLYKQTDSGQMFFGNLARKLPVFGKLNIKTAASNYARTLS
                     TLVYSGLPMIEALGITADTMKNALYKKALKNAREEVSKGVPLSEPITACGLFPPMVSH
                     MTRIGEETGDLEGMLTRLADYYDEEVELATQTVMAAIEPMIILVLALIVGVLVAAVMA
                     PMLTMYQAMDSL"
     gene            complement(4704..>5195)
                     /locus_tag="DHV81_08085"
     CDS             complement(4704..>5195)
                     /locus_tag="DHV81_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HCI94404.1"
                     /translation="RAALRQDPDVILVGEMRDLETISTAITAAETGHLVFSTLHTNSS
                     SDAIDRMIDVFPPHQQQQIRVQLSGVLEGIVAQHLLPMVNGGRIAAFEVLLANNAIRN
                     LIREAKAFQIPSIIQTSKREGMMMMDDCILQYYSQGLIAPETAVAYAHDPVSMASRVH
                     LYY"
BASE COUNT         1278 a         1259 c          943 g         1678 t
ORIGIN      
        1 ctcttgtcct tttgcagctt ttttacaata agagaaatta tcttttgtct ttctcctaat
       61 tctctgcctt catttttctg atcttcccat gctttacaca tatcaatcac ctcttctttc
      121 tcatctatgt ctatatccgt tcctgcaaac agattgattg cctcgactgt ctctctgtcc
      181 acactgctaa acttgtcatc attatgaagc acttcctgca tcttttcttt atcatacgca
      241 ttctgcaata cttcaaaaag ctgcctgatg gaggtcctga atccggtaaa gtctgttatc
      301 tccctcggtg ccagcagatt aattctgtaa tccggtatga atggcagaag ctcttcatcc
      361 acatcaccaa acatatctga cagctttctt ggaccatccc attcctttgt tccgagatat
      421 actgtaatcg taatcaccgg tatcagctta tcctctttcg taaatccaga gagaaattca
      481 tccgatgttg ccgtatcctt ttccttgcgg tgctttttgg cagtttcctt tacctggttg
      541 ccatatgcca tgacatcata aaacatgttt tttaccggaa tggcatagtg tatatcggac
      601 tgattttcta ctcctatcag tacgacatat gatttgccgg tatatttgat gctggcattt
      661 ttgaagatgt ccctcatctt ctgcatcgct atggtctttt tgtcaagccc tagtgcggtc
      721 agaacctctg tcgtgtccct ttccttaagg ttttccggca ggataacctc tcttcctcca
      781 tacagtatat tgttgcacag atccgcaaac ctggcattat ctgcaaagta ttcctttgcc
      841 attgagtcct ttgcgctcaa tccttctcct cctgttctct tgtgtgagta ggaaaatccg
      901 gtgccaatga tggtacagtg gtacagttta tgaagtatac atgtgcagtc cttctttatg
      961 ttatctatat gtttacatta tgattatcac actgtataca gttcatccgc cttttgacta
     1021 cattttctgc attctgtcta catacggcat ttcaattttc ctgctgttga ttggtaatta
     1081 aagcatcgaa aacttccatg tcctttgtag aatgatgcga agtggtctgc cctcttcaaa
     1141 gtggtgagca cctgaaaaac tgttatgtgg tgccgcttac agaaatataa tgctttgagt
     1201 tgattgtagc atagcggcag ttctttgtca tcataatttt tctttttcca tacttatatt
     1261 ttttcatact gtttctgcac tgcacgccta tcagcctgca ttctgccaca gtgcaagggg
     1321 ctttatcacg tgagaccagc tggggcacgc tttctctcgt gagcgaatag cgaagctgct
     1381 ttagatactg ttgaattatg cacacgatac acagaaatgc cctgccacgg atggcagggc
     1441 aaggagaacc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cgcgtttcga
     1501 cggtttcgtc gggcatcgta aatctttaag gcataattcc tacagtatct tggcagtgca
     1561 gcgtagctca agcgagagag tgtgcaccag cctgggctca ggggattaag ccccgtccta
     1621 ctgtcagtat cccttagcca aaaaagaagc agcctctgtg ctcccgccca aagactgctt
     1681 catattgatt atttttaatt ttcaactgct gctatgccag cttcttattt ttctcgagaa
     1741 gctcatagat tttctcaacg tcataatctg gcttcatgga caatgctgct tcgtaaattg
     1801 gagcgataac ctcctctttt tcttccaggt catctgctat ttccgccacg ctcttgtcct
     1861 tttgcagctt ttttacaacc agtgagatta tcttttgttt ttctccgatt tccttaccct
     1921 cttctgttgc aacctcttcc cagtaatcac tcacattaca catctcgctc atcctccctt
     1981 cgagttccat gctcatttta agtccataat cttcttcaag tatcttcttt ttctcgataa
     2041 actctgactt tgagaacagc tcttccagca ttcctatgag tttattctta gactgctttt
     2101 tattttttgt tgctctcgtc cggacattta tcagtgcacc atttaatttg tcaatctgtg
     2161 gtattttctt tattttacca taaatcaggc tggatttcat cccaaattcg attattgagt
     2221 cctctgattt ttttgtatcc atacatatcc atatggtata tacggatttt aagtcatcat
     2281 aattcgaatt tacaaactca gtacccttct gctcggaaat cattctgccc atgtaatagg
     2341 caatccggtt ttccaaccgg tagcccagct ttgatttttt actgctcttc tgggcttcaa
     2401 tattgatgat aaattctctt ctttttccat tataatacaa gggaaatttg atgtcgtaaa
     2461 taatttttcc atcatcctca tcaactgact cattatttac gttctggatt ttaactccaa
     2521 gcctttccgg aaatcttagc attactgtcg gctcaccaat gcacggaatt attttctcgg
     2581 cttccatttc cttaaattcc ggcacgagcg cctgaactat ataggctgct atctgcggat
     2641 ctgccagaat acacttcacg tagtcgtcac tggttgcctt gtcctgctct atagagctaa
     2701 gagttctgga caaatttgtc ttcacttttc tgtatatcat acacttctcc tgtttccaca
     2761 tgaaatctgc atacatatca ttttttatag ccccattttt tgtgcataca taatttcaaa
     2821 tgataataac cctttatatt cctcaaataa atgcccgcca cgcatagaaa cgaatagact
     2881 ggtactttag ctgatattta tttttaacac acaatcggat ttgttgctga cacagcatga
     2941 taaatcaacg ggtaaagaac atccccgaac catgtattcg aattgtagcc tattgtataa
     3001 cagttgtcaa tgtatttttt cgccacagcg taaggggctt catcacatga gaccggcggg
     3061 ggcacgctct ctcgcatgag cgcatagcga agctgcctag atactgttga attatgcaca
     3121 cgatacacag aaatgagctc ccacggatgg gagctcaagg agaaccgcgc cacggacggc
     3181 gcgtttcgac gttttcgtta agcatagtaa acctataagg cataattcct acagtatctt
     3241 ggcagtgtag cgtagctcac gcgagagaat gtgcaccagc cagggctcat gggatgaagc
     3301 cccgtcctac tgccaatgtc tctgcaccaa aaaagcagcc tttgagcttc acactcaaag
     3361 tctgcttctt ttttatttta taaactgtcc atagcctgat acatggtaag cataggagcc
     3421 ataacggctg ccacaagcac accaacaata agtgccagaa caaggataat cataggctca
     3481 atagctgcca taactgtctg ggtagccaac tcaacttcct catcatagta gtctgccagt
     3541 cgggtaagca tgccttccaa gtcaccggtt tcctcaccaa ttcgcgtcat gtgtgaaacc
     3601 ataggtggaa acaatccaca cgcagtgatt ggctcagaca gtggaacacc cttacttact
     3661 tcttcccttg catttttcag ggcttttttg taaagcgcat tcttcatggt gtcagcagtg
     3721 atgccaaggg cttcaatcat cggaagtccg gagtacacaa gtgttgaaag tgtacgcgca
     3781 tagttactgg ccgctgtctt gatgtttagc tttccgaaaa ccggaagctt tcttgcgaga
     3841 ttaccaaaga acatctggcc gctgtcagtc tgcttataaa gcttgatggc tataacaatt
     3901 gctgctatga cagcaatcac aatataccag tatccggtga caaaatcact catcgcaaca
     3961 actgcctttg tgatgcccgg cagctctgtt cccaggtcat tgaacatctc tgagtagctt
     4021 gggatgacct ttacaagcat tacaataaca accgcaatag ctacaatcac aacgataatc
     4081 ggataaactg ccgctttttt tatcatgccg ttaagctttg ctgatttttc gaagtgtgca
     4141 gacattctgt caaaagctac atcaatcttg ccggatgctt ctcctgccgc aaccatgctc
     4201 accatgatgt cagggaaaac attcgggtat ttttttagac catccgagag agtctcacct
     4261 ttttgaatct cggcatgaac acctctgata gctttcgcca taaccttatt ctcagtctga
     4321 tcggataaca tatccagcgt atcaagaatc gtaacaccgg cattgaccat actggtgaac
     4381 tggcggcaga ataccgatag atctctcggc tttacctttc ctcctacact gaatgagatt
     4441 tccttggtca gtgctgttgc cttggtgagg cttatgacca tgttgccatc catcttaagc
     4501 ttattttttg cctctgcttc tgactcggct actatatttc cttttctttc ttttccgctc
     4561 tggtctataa tctgatatgt ccagtcagcc actttcttct cctccttata cctattctgt
     4621 gcaacctcca gataaattaa atcataattt attaagactc cacaacatta taccataata
     4681 gtaaaataca tacgattatg ccatcaatag taaaggtgta cacggctagc catgcttacc
     4741 ggatcatgtg catatgcaac tgcagtctcc ggcgctataa ggccctggct gtaatactgt
     4801 aaaatacagt catccatcat catcatacct tctctctttg aggtctgtat gatagatgga
     4861 atctgaaatg cttttgcctc acgaattaag tttctgatgg cattgtttgc aaggagcacc
     4921 tcaaatgctg caattcgtcc gccatttacc attggaagca gatgctgtgc aacaattccc
     4981 tctaaaactc ctgagagctg cactcgtatc tgttgctgct ggtgtggtgg aaaaacgtca
     5041 atcatacggt caattgcatc tgatgaactg ttggtatgca gtgtagaaaa tacaagatgt
     5101 cctgtctccg ccgctgtaat agctgtagat atagtttcaa gatctcgcat ctctcctaca
     5161 agtattacat ccggatcctg tcgcagtgca gctct
//
LOCUS       DPUF01000231            2850 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_33533, whole
            genome shotgun sequence.
ACCESSION   DPUF01000231 DPUF01000000
VERSION     DPUF01000231.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_33533"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(395..1783)
                     /locus_tag="DHV81_08090"
     CDS             complement(395..1783)
                     /locus_tag="DHV81_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94405.1"
                     /translation="MNLPIEFEKKMKAFLGDEWDDFLYSYDNNRFQALRFNTLKVQSP
                     EERMRILKTLKISSDKKVSWADEAYYFDENVRPGKHPYHEMGLYYIQEPSAMSAAALL
                     APKPGMRVLDLCAAPGGKSTQLATYLGDSGLLVSNEINTQRSRILSQNIERMGIKNAI
                     VTNEDSFVLASHFPGFFNAIQVDAPCSGEGMFRKLPEAIEQWSMENVAICAARQKEIL
                     DNAAVMLKPGGTIVYSTCTFSKEENEDVIEYFLERHPDFTLEEMERFWPHKVDGEGHF
                     VAKLVRRGCVDTDLKADRKTKKNKNSKNRKNETKPALTKENMKLLSEFLDETISEDMA
                     AWIKNSRLVMFGEQLYRLPDMEVDIKGLKVQRAGLHIGEFKKQRFEPSHSLALALKLN
                     DAKNVVKLTCDNPQTIGFFNGQSVMLSDEQAAECKKGWALVCVDGYPAGWGKVNGAQV
                     KNHYPKGLRNKI"
     gene            complement(1987..>2850)
                     /locus_tag="DHV81_08095"
     CDS             complement(1987..>2850)
                     /locus_tag="DHV81_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein SpoIID"
                     /protein_id="HCI94406.1"
                     /translation="SFEFIGTEEGIVAVNTVDVETYVRYVLPSEMPSTFDAEALKAQA
                     VCARTFVYSQMKNTQYALYGANIDNTTAFQVYNASEAKQSTDEAVKATAGQVVSCGGS
                     LITCYYFSTSAGKTEDMEVWSSSTPDFIHKVESVDDNSPYYRWTSELDLSAYNDPQYG
                     TATGISVDKTSDAGYVLSLTINYGNKSQTFTAENDIRKALGHYQKKVTLNDGSVRESM
                     SMIPSACFSVSAGANGHYTLSGGGFGHGIGFSQYGADKLAKAGSGYKDIIGYYYKDVT
                     VVDISSVRSEQ"
BASE COUNT          739 a          738 c          471 g          902 t
ORIGIN      
        1 caagcttgaa ttcgccgttt atgcgataaa aaacgcccct gcatggctat taaaccatgc
       61 agggacgaaa attccgcgat accaccctga tttccattga agaaatatcc aatggactct
      121 cattgatatg tgtaacgtac ataacacgtc atgaactact gtgcttcatc catgcagctt
      181 cagtgtgaac ttcaatcaat agcctttttg taagactgct ttcagccgat gacaatctct
      241 ctctggtcaa agtactataa tctactatgc accttcattg cttttattat atatagctgc
      301 gacatatata tcgcaaatgc ttatttctta ataacacata agtcatttta gcacaatctc
      361 tatattttgc aagtagaaaa tttttctatt ttctttatat tttatttcta agccccttag
      421 gataatggtt tttaacctgt gcaccattca ccttgcccca gccggccgga tatccatcta
      481 cacatacaag tgcccagccc tttttgcatt cagccgcctg ctcatcagat aacataactg
      541 actgaccatt gaaaaagccg atagtctgcg gattgtcaca tgtaagcttt actacgtttt
      601 tagcatcatt tagcttaagt gcaagtgcca gcgaatgcga cggctcaaag cgctgttttt
      661 taaactctcc tatatgaagc cctgctcgct gcaccttaag ccctttaata tcaacttcca
      721 tatcaggcag cctgtagagc tgctccccaa acatgacaag ccttgagttt tttatccatg
      781 ctgccatatc ctctgatatg gtctcatcaa gaaactctga gagaagcttc atattttctt
      841 ttgtgagagc cggctttgtt tcatttttcc tgttcttact atttttgttc tttttggttt
      901 tcctgtcagc tttaaggtct gtgtccacac agcctcttct cacaagcttt gccacaaaat
      961 gtccctctcc gtccacctta tgcggccaga agcgctccat ttcttcaagt gtaaaatcag
     1021 gatgtctctc caaaaaatac tcaattacat cctcattttc ttccttcgag aaggtgcagg
     1081 tgctgtacac tatcgtacct cccggcttaa gcatcaccgc agcattatcc agtatttcct
     1141 tctgccttgc cgcacagatt gccacatttt ccatgctcca ctgctctatt gcctcaggca
     1201 gctttcgaaa cattccctct cctgagcaag gtgcatccac ctgtatagca ttaaaaaagc
     1261 ctgggaaatg tgaagcaagc acaaagctgt cctcatttgt cacaatcgca tttttgattc
     1321 ccatacgctc tatattctgc gacaaaatcc ggcttctttg tgtgttaatc tcatttgata
     1381 ccaaaagtcc gctgtctcct aaatatgtgg caagctgtgt gctctttcca ccgggtgccg
     1441 cacacaggtc aagcactctc atccccggct ttggagctaa aagtgcagcc gccgacatgg
     1501 cacttggctc ctgtatgtag taaagcccca tctcatgata cggatgcttt cccggacgca
     1561 cattctcatc aaagtaatat gcctcatctg cccacgaaac ctttttatct gaggatattt
     1621 taagtgtttt caatatccgc attctttctt ccgggctttg tacctttaat gtattaaatc
     1681 tgagtgcctg aaacctgtta ttgtcatagc tgtataagaa atcatcccac tcatcaccca
     1741 gaaaggcctt catctttttt tcaaactcta tcggtaaatt cattgtcaaa tcctcttgtt
     1801 ttcttacgaa ataattacaa tgtatcttac tatcacacac atagtcatgt atctaagcac
     1861 ttcgatatta ccacatttct actatccatg ctatcaattt tttttaacgt aaaaattcac
     1921 ctttaaatcg gctatagtgg ctgttaaata taataaaatc ccgaaagtta taataattga
     1981 gacatatcac tgctcactgc gtacactgct tatatccacc actgtcacat ccttataata
     2041 atatccgatt atatccttat agccgcttcc tgcctttgcc agcttgtctg ccccatactg
     2101 gctgaagcct attccatggc cgaagcctcc tccggacagt gtatagtgac catttgcacc
     2161 ggcactcaca ctaaagcagg cgcttggtat catcgacata ctctccctca cagagccatc
     2221 gtttaatgta acttttttct gataatgccc taacgccttt cttatatcat tctccgccgt
     2281 aaaggtctgc gatttgtttc cataatttat tgtgagcgaa agtacataac cggcatccga
     2341 cgtcttatcg acagaaatac ctgttgctgt gccgtactga ggatcattgt atgccgataa
     2401 atcaagctcc gatgtccatc tgtagtatgg cgaattgtca tccactgact ccaccttgtg
     2461 tatgaaatcc ggtgtactcg aagaccatac ctccatatcc tcggtttttc cggcagacgt
     2521 agaaaaataa taacatgtta ttaagcttcc tccacatgag acaacctgtc ctgctgtagc
     2581 ttttacagcc tcatcggtcg actgctttgc ttctgaagcg ttatagacct gaaaagccgt
     2641 tgtattgtca atatttgctc cgtatagcgc atactgtgta tttttcatct gtgaatatac
     2701 aaaggttctt gcgcaaacag cctgagcctt aagcgcctct gcatcaaatg tagacggcat
     2761 ctccgacggc agcacatagc gcacatatgt ctccacatcg actgtattca ccgccacaat
     2821 accttcttct gtcccgataa actcaaatga
//
LOCUS       DPUF01000232            4868 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_15554, whole
            genome shotgun sequence.
ACCESSION   DPUF01000232 DPUF01000000
VERSION     DPUF01000232.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_15554"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="DHV81_08100"
     CDS             <1..534
                     /locus_tag="DHV81_08100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94407.1"
                     /translation="RRGNGALYLNKVYDDCRVENFYDDDINKNYVLGMVKRRLAKNIY
                     DNDYTEPAYVNNILKSLFMEPEEISTSPDIKELDIVTLYVFYAIFSKNEDYVGNIRNV
                     VLRMKLYVGQYSKLWRDYFDYFLQIIDSYQWKKELEINKKKDMILLYDCWANIPGRHR
                     NTLLYVKNKIDDYINQI"
     gene            512..763
                     /locus_tag="DHV81_08105"
     CDS             512..763
                     /locus_tag="DHV81_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94408.1"
                     /translation="MIILIKYEVPRKIEQGREKMKKIISNLSVVIFVIGCVIMEITTW
                     FIDSYVMFYIGIIIVIVGIIGMIIQGKFKKVIEFIIEYF"
     gene            885..1064
                     /locus_tag="DHV81_08110"
     CDS             885..1064
                     /locus_tag="DHV81_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94409.1"
                     /translation="MKNKIIIYVIVQFLLILCSIFVIPNVEGVLSIFTVLIVIVGNVL
                     NLIFLLKAKNRKESF"
     gene            complement(1396..3090)
                     /locus_tag="DHV81_08115"
     CDS             complement(1396..3090)
                     /locus_tag="DHV81_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94410.1"
                     /translation="MEILKLPVGIENFEDIRRSGFYYIDKTMLIEQTLNNWSKVTLFT
                     RPRRFGKTLGMSMLRSFFEIGTDKSLFDGLYISQDKSLCDEHMGKYPVIFISLKDVEG
                     LSYDEAFQVFSRIIGNEISKFSFLAESNKLTVLEKEQFKGLLHIEKGKFIFDKDTFTA
                     SLKLLSQLLYKHYGQKVVILIDEYDVPLDKAYQNGYYHEMVSLIRGLFGQALKTNDYL
                     QFAILTGCLRISKESIFTGLNNFEVVSIMDSMYDECFGFTDKEVQEILKYFNLSEHYA
                     DIREWYDGYHFGNTDIYCPWDVIRYCKSLCADPTAKPQDFWSNSSGNALVRSFIDKAD
                     VQTKDEIERLIADEYIEKEISQELTYDEIDKSIANLWSVLFTTGYLTKQGVTDDGKVR
                     LSIPNREIKNLFIKKIREWFSDTTANDGKTLEQFCNAFVDKNTEKIEELFGDYLWNTI
                     SIRDTAVAKDKKENFYHGILLGLLGYKASWLIKSNTESGTGYSDILVEVPNNRTGIVI
                     ELKYAENGDMDAACDEALNQIEKKSYVDKLKQDGMRNFIKYGIACFKKDCKVVVSK"
     gene            3275..4318
                     /locus_tag="DHV81_08120"
     CDS             3275..4318
                     /locus_tag="DHV81_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCI94411.1"
                     /translation="MKRFTAILLTGMMIFTLASCGKKAQDTPTEPATETQAAEKVEMP
                     TETEAVTEVVTEAAKETQQTEAQQEEQTAEQPDEEQNNSGDNNSSYQYVERASYENGE
                     SVSLNPSWQYADHSAINSGCAVMYKATANRKNIVVGVNAGHGTSGGTSVKTLCHPDGS
                     AKTTGGTTGAGATKAVAVSGGMSFNDGTPESSVTLRMAQILKDKLLAAGYDVLMVRDG
                     SDVQLDNVARTVICNNAADCHIALHWDGDGLSYDKGCFYISVPGGIKGMEPVASHWQQ
                     HDALGASLIEGLRAHGAKINGNGSMAIDLTQTSYSTVPSVDVELGNACSDHSDATLEN
                     LADGLVQGVEGYF"
     gene            4500..>4868
                     /locus_tag="DHV81_08125"
     CDS             4500..>4868
                     /locus_tag="DHV81_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI94412.1"
                     /translation="MTDNKSGEILIIEDDKDINDLLATALSKAGYRTRQAWSGTEGEL
                     LLRLDMEAYALVLLDLMLPGLSGEELLARIRQMDASLPVIILTAKDELDEKINLLVAG
                     ADDYITKPFEIKEVVARVAVQ"
BASE COUNT         1511 a          841 c         1076 g         1440 t
ORIGIN      
        1 agaagaggga atggagcttt gtatttgaat aaagtttatg atgattgcag ggtggaaaat
       61 ttctatgatg atgatataaa taaaaactat gttcttggca tggttaagag gagactggct
      121 aagaatatat atgataatga ttatacagaa ccggcatatg taaacaatat attaaaaagt
      181 ctttttatgg aaccggagga aatcagtact tcgccggata taaaggaact tgatattgtt
      241 acactgtatg tattttatgc tatatttagc aagaatgagg attacgtagg caatatccgt
      301 aatgttgtac tgagaatgaa attatatgta ggacaatatt caaaattgtg gagagattat
      361 tttgattatt tccttcaaat aattgattca taccaatgga aaaaagaatt ggaaattaat
      421 aaaaagaaag atatgattct gctatatgac tgttgggcaa atattccggg caggcatagg
      481 aataccttat tgtatgtgaa aaataaaatt gatgattata ttaatcaaat atgaggtacc
      541 taggaaaata gaacaaggac gtgagaaaat gaaaaaaata atttctaatt tgtcagtagt
      601 gatttttgtc ataggatgcg tgataatgga aataacaaca tggtttattg attcatatgt
      661 tatgttttat ataggaataa ttattgttat agtgggaatt attggaatga ttattcaagg
      721 aaagtttaag aaagtaattg agtttattat agagtatttt tagaaagttt tgctttcaaa
      781 attctgataa gtgcattgat atcattttgt ggagtgattt gtgaatttct tcaaaaaatg
      841 ctgatatatt agaaaatggt gaattaggag ggagaaatta tcggatgaag aataaaataa
      901 taatatatgt gatagtacaa tttttactaa ttttatgttc aatatttgtt attccaaatg
      961 ttgagggtgt attgagtatt ttcacagtgc tgattgttat tgttggaaat gttttaaatt
     1021 taattttttt attaaaagca aaaaaccgca aagagagttt ttgagcatta ttagcgtttg
     1081 aaccaagtgg taattttacc atttgatagt ttatataaaa agttaagaaa aggagaaaat
     1141 ggatatgaaa aataaccttg aacttatttt attagtaggt aactgggtag ttgttgcaat
     1201 acaggcgttt actatatttt tatatggaaa tagcatgggg ctgtttatca ttatgctgct
     1261 tgcattaata gctcaagtat ttggtgttta tgataataga aagaaataat taatatattg
     1321 acttacaaaa tgtgtattaa aagaatagta aacaggcgtg gagctagtct ctctacgcct
     1381 gtttaaatgc gtgccttatt tacttaccac aaccttacaa tccttcttaa agcaagcaat
     1441 tccatacttg atgaaattgc gcatgccatc ctgcttcagc ttatccacat atgatttttt
     1501 ctcaatctgg ttcagtgcct catcgcaggc tgcatccata tcgccgtttt cggcatattt
     1561 aagctctatc acgatgccgg ttctgttgtt tggaacttca acgaggatat cgctgtagcc
     1621 tgtgcccgat tcggtgtttg atttgatgag ccagcttgcc ttgtagccca gcagaccaag
     1681 cagtataccg tggtagaaat tttctttttt atctttggca actgcggtat cccttatgct
     1741 tattgtattc cagagataat cgccaaaaag ctcttctatt ttttcggtat tcttatccac
     1801 aaatgcattg cagaattgtt caagtgtctt tccgtcattt gcggttgtat cgctgaacca
     1861 ttcgcggatt tttttgatga aaaggttttt aatctcgcgg tttggtattg ataaacgtac
     1921 cttgccatcg tcagtcacgc cctgcttggt caggtaaccg gtggtaaaga gcacactcca
     1981 caggtttgcg atgcttttgt cgatttcatc gtaggtcagc tcctgggata tttctttctc
     2041 aatgtattcg tctgctatca gacgctcaat ctcatccttg gtctggacat cggccttgtc
     2101 aatgaagctt ctgaccagag catttccgct tgaatttgac cagaagtcct gcggctttgc
     2161 agtgggatct gcgcacaggc tcttgcaata tcttatgaca tcccacgggc agtagatatc
     2221 ggtgttgccg aaatgatagc cgtcgtacca ttcccttata tctgcataat gctctgaaag
     2281 attaaaatac ttaagtattt cctgaacttc tttatctgta aagccaaagc actcgtcata
     2341 cattgaatcc atgattgaaa cgacttcaaa gttgtttaat cctgtaaata tgctttcctt
     2401 cgatatgcga agacagcctg tcaaaatagc aaactgtaag taatcgttgg tctttagtgc
     2461 ctgtccgaac aagcctcgta tgagagagac catctcatgg tagtagccgt tctggtatgc
     2521 cttgtcgagt ggcacatcgt attcatctat caggatgact accttttggc cgtaatgctt
     2581 atataaaagc tgggaaagaa gctttaatga agcggtaaat gtatccttgt caaaaataaa
     2641 ctttcccttt tctatatgaa gtaatccttt gaactgttct ttttccaaaa ctgtcaattt
     2701 attactttct gccaaaaagg agaatttgct gatttcattt cctataattc tggagaatac
     2761 ctgaaatgcc tcatcatatg ataaaccctc gacatcctta agtgaaataa atatcacagg
     2821 atatttgccc atatgctcat cacaaagaga tttatcttgt gaaatatata atccgtcaaa
     2881 cagtgatttg tcagtgccaa tctcgaaaaa tgagcgcagc atgctcatgc caagagtctt
     2941 tccaaagcgc cttggacggg tgaacagcgt tactttactc caattgttta aagtctgctc
     3001 gataagcata gttttgtcta tataataaaa tccagacctt cgtatatctt caaaattttc
     3061 gattccaaca ggcagtttta atatttccat gtgagcctcc ccggttaaaa tatgttcgat
     3121 acattcacat atagtataac tgatttttag aggaattaca atttctattg actatttaaa
     3181 actggcccct aaaatgaaag taatgttgcc taatacttga acaatatcat ggggttgtct
     3241 ataatgtagt aaaaagaaaa tggagtttta ataaatgaaa cgatttacag caatattact
     3301 tacaggcatg atgatattta ccctcgcatc atgcggcaaa aaagcacagg atacaccaac
     3361 agagcctgca acagagacac aggctgcaga gaaggtcgag atgcctacgg aaacggaagc
     3421 cgtgacagag gtggtaacag aggccgcaaa agaaacacag cagacagaag cgcagcagga
     3481 agaacagact gccgaacagc cggacgagga gcagaacaat tcaggtgata ataattcctc
     3541 ttatcagtat gtagaaagag catcctatga gaatggagaa tcagtgtcac taaatccttc
     3601 atggcagtat gcagaccatt cagcgataaa ttcaggctgt gcggtcatgt acaaggctac
     3661 agcaaaccgc aaaaacatag tagtaggtgt caatgccgga cacggtacaa gcggaggaac
     3721 ttcggtgaag acactttgtc atccggacgg ctcggcaaag accacaggcg gtacaacagg
     3781 cgcaggcgca acaaaagcag tagcagtttc aggtggtatg tcctttaatg acggtacacc
     3841 ggagagctct gtcacactcc gtatggcgca gatattaaag gacaagctgc ttgcggcagg
     3901 ctatgatgtg cttatggtaa gagacggaag cgatgtgcag cttgacaatg tggcacgtac
     3961 tgtcatctgc aataatgcgg cggattgtca tatcgctctg cactgggatg gcgacggact
     4021 aagctacgat aagggctgct tttacatatc agtgccggga ggcataaagg gaatggagcc
     4081 agttgcatca cactggcagc agcatgatgc acttggggca agcctgatag aaggactgcg
     4141 cgctcatgga gcaaagataa acggtaatgg ctcaatggcg atagacctga cacagacctc
     4201 atacagcacg gtgccgtcag tggatgtaga gcttggcaac gcatgctctg accacagcga
     4261 tgccacactt gaaaatctgg cagacggact ggtgcaggga gtcgagggat atttctagaa
     4321 tataaaaagt tactgttgtt ttttggcgtg tattggcact aaactaaagt ttgcaaaaac
     4381 aaccgaatag cggattcaaa tgaataggac gcatagtgag ataaatacgt ccggcatgat
     4441 tttgtcagta tagaggtgtt attatagaag tggtacaaca gatagagaaa gggaatatta
     4501 tgacagacaa caaatccggg gagatactca taatagagga tgacaaggac attaatgatc
     4561 tgcttgcgac agcgctttca aaagccggat ataggacaag acaggcatgg tcaggcacag
     4621 agggagagct tttgcttagg ctggacatgg aagcctatgc gcttgtgcta ttggatctga
     4681 tgcttccggg gctttcaggg gaggagcttc tggcacggat acggcagatg gatgcgtcac
     4741 ttccggtgat aatccttacg gcaaaggatg aactggatga gaaaatcaat ctgctcgtgg
     4801 caggcgctga tgattacatc acaaagccgt ttgagataaa ggaggtcgtt gccagagtgg
     4861 ctgtgcag
//
LOCUS       DPUF01000233            3464 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_33352, whole
            genome shotgun sequence.
ACCESSION   DPUF01000233 DPUF01000000
VERSION     DPUF01000233.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_33352"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="DHV81_08130"
     CDS             <1..285
                     /locus_tag="DHV81_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation subunit of DNA-dependent DNA
                     polymerase"
                     /protein_id="HCI94413.1"
                     /translation="DYKNYYYLPKEDMAIHKSVAAYVDHEYREKCKAYNCYVRKTGTF
                     IRQYSDFMKPEFRFDIKDKYSYFLLTEDFINSKQMVLSYVKHITDHLFNL"
     gene            complement(293..961)
                     /locus_tag="DHV81_08135"
                     /pseudo
     CDS             complement(293..961)
                     /locus_tag="DHV81_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742481.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheR"
     assembly_gap    527..590
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    962..1052
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1160..2275)
                     /locus_tag="DHV81_08140"
     CDS             complement(1160..2275)
                     /locus_tag="DHV81_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCI94414.1"
                     /translation="MSEMSFEQMLEESFKTIRNGEVVEGTVIDVKPDEIFLNIGYKSD
                     GIITRNEYTNEPNVDLTTVVKVGDKMEAKVVKLNDGEGQVILSYKRLQAEKGSKRLEE
                     AFENQEVLKAKVTQVLAGGVCVSVDETRVFIPASLVSDTYEKDLNKFKDQEIEFIITE
                     FDPRKRRIIGNRKVLLVEQKAKQQEELLANLHVGDVIEGTVKNVTDFGAFIDLGGVDG
                     LLHISEMSWGRVENPKKVFTVGQTVRVFVKNINGSKIALSMKFDDENPWNGATEKFAP
                     GNIVKGKVARMTDFGAFIELAPGVDALLHVSQISKDHVEKPSDVLKIGQEVEAKVVEF
                     NEDDKKISLSMKALLDSEDDGADVADVNIDEVASDNE"
     gene            complement(2256..3116)
                     /gene="ispH"
                     /locus_tag="DHV81_08145"
     CDS             complement(2256..3116)
                     /gene="ispH"
                     /locus_tag="DHV81_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCI94415.1"
                     /translation="MGELTIAKSAGFCFGVTRAVNMVYEAIEKENVPIYTYGPIIHND
                     EVVKDMEKNGVTVINDLDELSSHEKGVMIIRSHGISKAEYDRIKNCGFEVLNATCPFV
                     SKIHRYVEDYSSKGYDIIIVGSPKHPEVCGIKGWADEKCHVTIINSPEDAENYMKNST
                     KKLCIVSQTTFNYNKFQDIVEIIAKKGYDITVLNTICNATEVRQTEARKVAQCSDVMI
                     VIGDRHSSNTQKLFEICKNECKNTYYIQTSDEMDYTQIRSNNNVGITAGASTPNNIIE
                     EVSKNVRNEL"
     gene            complement(3120..>3464)
                     /locus_tag="DHV81_08150"
     CDS             complement(3120..>3464)
                     /locus_tag="DHV81_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCI94416.1"
                     /translation="REKLVELQRQLAARENVVMDGRDIGTVVLPDANVKIFLTASSKV
                     RAKRRFDELTAKGEKCDIDAIEKDIIERDHRDMTRETSPLKQADDAVLLDSSDMTIDE
                     VVDRMKQLVKEA"
BASE COUNT          919 a          798 c          526 g         1066 t
ORIGIN      
        1 gattacaaaa actactacta tctgccaaag gaggacatgg ccatacacaa gagcgtcgca
       61 gcatatgtcg accacgaata ccgcgaaaaa tgcaaggcgt acaactgcta tgtgcgcaaa
      121 accggtacct tcatcagaca gtactctgac ttcatgaagc cagagtttcg attcgatata
      181 aaggataagt acagctattt cctgctgact gaagatttta taaattcaaa acagatggta
      241 ctgtcatatg taaaacatat aacagaccat ctgtttaacc tctgataaga tatcatggct
      301 tcctgaaata gaaactgctt aatctctcaa agccgagctc acgatatgta gttatctgct
      361 cagtactgcc tataaacagt attcccttgg attttaaagt attgaagaaa tttttgtata
      421 tcatatcctt cgcatcatcg gtaaaataaa tcaccacatt cctgcacagg ataagatcgt
      481 aatcctttgg atatgaatcc ttcaacagat tgtgctcctt aaaggtnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcttcaggtc
      601 agccggcaca ttttctacag actttgcgct gtaaagcccg gctttagcac tcgcaatcac
      661 ctggtcgtca atatctgtag ccagtatctt tatacgagac agtggaacat gcttcgaaaa
      721 agccatggca agcgaatacg gctcgtcccc tgttgagcat gccgcgctcc atatcttaat
      781 catccctgta ttgttcttta atatctcagg aatgacctct ctgtccaaaa aactccactg
      841 atcaggattc ctgtagaact cagatacatt gatagtgata aaattgataa actgtttaaa
      901 cttaacctta tctgtagaaa tcatatcaat gtatgtctca taagaatcag ccccattttt
      961 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttttaaa ccattcataa tgatcaaaca
     1081 tcacaatcct ccaatatgtg ccacccgata caaataaaag ctgccatata attatatatg
     1141 acagctttaa aatcaaaaac tactcgttat cagaagcaac ctcatcgata tttacatctg
     1201 caacatctgc tccatcatcc tctgaatcaa ggagagcctt catgcttaag cttattttct
     1261 tatcgtcctc attgaactca acaaccttag cctcaacctc ctgaccaatc ttgagaacat
     1321 ctgatggctt ctcaacatga tcctttgaaa tctgtgaaac atgaagaagt gcgtcaacac
     1381 caggagcaag ctcgatgaat gcaccaaaat ctgtcattct ggctacttta ccctttacga
     1441 tattgccagg agcaaatttc tctgttgcac cattccatgg attctcgtca tcaaacttca
     1501 ttgaaagagc aatcttagaa ccattgatgt tcttaacaaa tactcttaca gtctgtccaa
     1561 cagtgaatac cttcttaggg ttctctactc tgccccaaga catctctgag atatgaagta
     1621 atccgtctac tccgccaaga tcgatgaatg caccgaaatc tgtaacattc ttaactgttc
     1681 cttcgattac atcgccaaca tgaagatttg caagaagctc ctcctgctgt ttagccttct
     1741 gctcaacgag taataccttt ctatttccga taatacgtct ctttcttgga tcgaactctg
     1801 tgatgataaa ctcaatctcc tgatccttga acttgttgag atccttctca taagtatctg
     1861 atacaaggct tgcagggatg aatactcttg tctcatcaac actgacacat acaccaccgg
     1921 ctaatacctg tgtaactttt gcctttaata cttcctggtt ctcaaaagcc tcctcaagac
     1981 gcttgctgcc cttctctgcc tgaaggcgct tgtaagaaag gataacctgg ccctcgccat
     2041 cgttaagctt aactaccttg gcttccatct tgtcgccaac ctttacaaca gtagtaagat
     2101 ctacgttcgg ctcatttgtg tactcgtttc tagtaataat tccgtctgat ttgtatccaa
     2161 tatttaagaa aatctcgtca ggtttaacat caataacggt accttctacc acttctccat
     2221 ttcttatagt tttaaaagat tcttccagca tttgttcaaa gctcatttct gacattcttt
     2281 gaaacctcct caatgatatt atttggggtt gaagcccctg ctgtaatacc tacgttattg
     2341 ttggacctga tttgtgtata atccatttcg tcacttgtct gtatatagta agtatttttg
     2401 cattcgtttt tacatatttc aaatagcttc tgtgtattgg agctgtgcct gtcgcctatg
     2461 acaatcatga catctgagca ctgtgctacc tttctagctt cggtctgtct gacctctgta
     2521 gcattacaga ttgtatttaa aacagttata tcataacctt tttttgcaat aatttcaact
     2581 atatcttgaa atttattgta gttaaatgtc gtttgtgaca ctatgcatag tttttttgta
     2641 ctatttttca tgtaattttc ggcatcctca ggcgaattta tgatggttac atggcatttt
     2701 tcgtctgccc agccctttat tccacacacc tcagggtgct ttggactgcc gacaattata
     2761 atatcatagc cctttgagct gtaatcctcc acatatctgt gaatttttga tacaaaagga
     2821 caggttgcat taagcacctc aaagccacag ttttttatcc tgtcatactc agccttgctt
     2881 ataccgtgtg aacggataat catcacaccc ttttcatgag aagaaagctc atcgagatcg
     2941 tttatgacgg ttacgccatt tttttccata tcctttacga cctcatcatt gtgaataatc
     3001 gggccatagg tgtatatagg cacattttcc ttttcaattg cctcatagac catatttact
     3061 gctctcgtca ctccaaaaca aaagccggcg ctctttgcga tagtaagctc tcccatacgc
     3121 tatgcctctt tcacaagctg tttcattcta tctaccacct catcgattgt catatcagac
     3181 gaatcaagca gcacagcatc atccgcctgc ttcaaaggtg atgtctctct tgtcatatcc
     3241 ctgtggtctc tctctataat atccttctcg attgcatcaa tatcgcattt ctcacccttt
     3301 gcagtaagct catcgaagcg tctctttgca cgtactttag agcttgctgt taaaaatatc
     3361 ttgacattgg catcaggaag cacaacagtt cctatatctc ttccatccat caccacattc
     3421 tctctggcag cgagctgtct ttgaagctca acaagctttt ctct
//
LOCUS       DPUF01000234            3846 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_19028, whole
            genome shotgun sequence.
ACCESSION   DPUF01000234 DPUF01000000
VERSION     DPUF01000234.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_19028"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="DHV81_08155"
     CDS             <1..705
                     /locus_tag="DHV81_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCI94417.1"
                     /translation="EAFRSGKTIDKLFVLDGCQDGPIKSITREARKHDAIINYVAKER
                     LDQMSETGKHQGVIAVSAAYEYAEVSDILENAERKGEPPFIIILDGIEDPHNLGAIIR
                     TANQAGAHGIIIPKRRAVGLTPTVAKTSAGAINYTPVAKVTNISNTIKELKEKGMWFV
                     CADMGGTTMYDLNLTGPIGLVIGNEGDGVSKLVKENCDMTASIPMVGDIDSLNASVAT
                     GVLAYEIVRQRLKSGK"
     gene            768..1376
                     /locus_tag="DHV81_08160"
     CDS             768..1376
                     /locus_tag="DHV81_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HCI94418.1"
                     /translation="MVEDYSAYTDEELIMRIHNDKNDHGDNNEIMDYILEKYKPLVRK
                     KTNALYLIGGENDDLIQEGMIGLFKAVRDYKSDKEASFYSFAQLCITRQLSSALEASN
                     RKKHMPLNTYISFSQSDSDGTEFEEMLQDDIASPEQLLIEKEKFKEFKEQLWNKLSNM
                     EKKVLQLYLEGNNYTSIAHMLGKSDKSIDNALSRIRQKLKRK"
     gene            1389..2045
                     /locus_tag="DHV81_08165"
     CDS             1389..2045
                     /locus_tag="DHV81_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCI94419.1"
                     /translation="MEFSRIGEHTIKCVISEEEIYDLGYTMDEIMSNGERTQEFMNQI
                     FDMASEEFGTDFNSGIKTVRADFLADHTLSLTFSEHPATEGMMEHLKDIVNGLLNSLP
                     AETAENEKNKKGRTTAKKQDMVSVVMFIFEKFDVAVRFAKMIKGLPMPFSQLFKFEGE
                     YFMSMDLSGYNEHELEKMSVIADEYADDIIAGADKRAFLMEHAKVILDDKAIESLAQL
                     "
     gene            complement(2124..2483)
                     /locus_tag="DHV81_08170"
     CDS             complement(2124..2483)
                     /locus_tag="DHV81_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCI94420.1"
                     /translation="MARILIVDDAAFMRMMLKDILTKGGFEIAGEAADGNEAVAKYNE
                     LKPDLVTLDITMPNKDGIQALKEIKAADPNATCVMCSAMGQQSMVIDAIQSGAKDFIV
                     KPFQADRVIESIRKVLG"
     gene            complement(2488..3066)
                     /locus_tag="DHV81_08175"
     CDS             complement(2488..3066)
                     /locus_tag="DHV81_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCI94421.1"
                     /translation="MQNISRENADIIGEVANICIGNAATTLSMILQKPTNITTPIVEV
                     LKKEDALDHDDRILIKVPYTKGLDGTNLLIIKLNDALVIANLMMGGNGSNVSDMTLDE
                     ITLSAISEAMNQMCGTIATSMSSLLNEPTDIGFPLINSSYQKTFDIPDELCNGTDIVK
                     VTFRLTVGDLIDSDLLQLYPISIVKQIIKQEV"
     gene            3197..>3846
                     /locus_tag="DHV81_08180"
     CDS             3197..>3846
                     /locus_tag="DHV81_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="simple sugar transporter substrate-binding
                     protein"
                     /protein_id="HCI94422.1"
                     /translation="MGETEYSEALKLGKKEYRARIAKGQFPYLPVLDEILSEADIQTE
                     QNMGLVHVPLDFVVGTSTMGRTYSFAANFMPILDWETEFAVKWSNLSDAQMNEGIRDP
                     IKAFEYMNRYYVLEGNKRVSVLKYFNAVSIPAIVTRKIPKLSDDYDVRLYYEYMKFNE
                     ITGLCSVEFTKLGNADKLLSLVGKEGRWDDETKEKFAKVMFDFSKVYNFRGGDRLD"
BASE COUNT         1199 a          666 c          890 g         1091 t
ORIGIN      
        1 gaggctttcc gttcgggaaa gacaatagac aagctttttg tgcttgacgg ctgtcaggac
       61 ggaccaataa agtcaatcac aagagaggcc agaaagcatg atgcaatcat aaactatgtt
      121 gcaaaggaaa gacttgatca gatgtctgag acaggcaagc atcagggtgt cattgcagtt
      181 tcagcagcat atgagtatgc cgaggtttcg gacatactgg aaaatgccga aagaaagggc
      241 gagccgcctt ttatcatcat acttgacggt atagaggatc cacacaatct tggcgcaata
      301 atccgtaccg caaatcaggc gggtgcacat ggcatcatca taccaaaaag gcgtgcagtg
      361 ggacttaccc ctacagtggc aaagacgtct gcaggtgcga taaactatac acctgttgcc
      421 aaggttacca atatctcaaa taccataaag gagcttaagg aaaagggtat gtggtttgta
      481 tgtgctgata tgggtggcac taccatgtat gacttaaacc tcacaggtcc tataggtctt
      541 gtaataggca atgagggtga cggagtcagc aagcttgtaa aggaaaactg tgatatgaca
      601 gcttctattc ctatggtggg cgatattgac tcactgaatg catcggttgc gacaggcgtg
      661 cttgcatatg agatagtcag acagagactt aagagtggaa agtagattgc atgtcgcgag
      721 atgagtggtg tgtattatga attgtatatt tatagaatag gaagcatata gtggaagatt
      781 acagcgcata tacggatgaa gagcttatta tgaggattca taatgacaaa aatgaccatg
      841 gtgacaacaa tgaaatcatg gactatatat tggaaaagta taagccgctt gtgcgtaaaa
      901 agacgaatgc actctacctg ataggcggtg agaatgatga ccttattcag gagggcatga
      961 ttggcctgtt taaagcggtc agagactata agagcgacaa ggaagcatct ttctacagtt
     1021 ttgcacagct atgcatcaca aggcagctta gctcggcgct tgaggcttca aaccgaaaga
     1081 aacatatgcc gcttaacaca tatatatcct tttcgcagag tgacagtgat ggtacagagt
     1141 ttgaggaaat gctgcaagat gatattgcaa gtcctgagca gcttctcata gagaaggaaa
     1201 aatttaagga atttaaggaa caattgtgga ataaactgag taatatggag aaaaaagtgc
     1261 tacaattgta tctggaagga aataattata catcgatagc gcatatgctt ggaaaatcag
     1321 acaaatcaat tgacaatgca ctatcccgta tcagacaaaa gctaaaaaga aagtaggtga
     1381 agcttgttat ggagttttca agaataggtg aacacactat aaaatgtgtt atttcagaag
     1441 aagaaatata tgatctggga tatacaatgg atgaaattat gagcaatggc gagcgcaccc
     1501 aggaattcat gaaccagata tttgacatgg cttcagaaga gtttggaact gattttaatt
     1561 cgggaatcaa gactgtgaga gcagattttt tggcggatca cacgctttct ctgacatttt
     1621 ctgaacatcc tgctactgag ggcatgatgg agcatcttaa agatatcgtt aatggactgc
     1681 ttaattcgct gccggctgag actgcagaga atgagaaaaa taaaaaaggc aggacaactg
     1741 caaaaaagca ggatatggtg tctgttgtaa tgttcatttt tgaaaaattt gatgtggctg
     1801 tcagatttgc aaagatgatt aaaggactgc caatgccatt ctcgcagctt tttaagtttg
     1861 agggagagta ctttatgagc atggatttat cgggatataa tgagcatgag cttgaaaaga
     1921 tgtctgtcat agcagatgag tatgcagatg atatcattgc aggggctgac aaaagggctt
     1981 ttcttatgga gcatgcaaag gttattcttg atgataaggc tattgagagt cttgcgcagc
     2041 tgtgaaggta ctgaacagtt acaaaaatat aataaaacaa ggtctgtcag ctatactgac
     2101 agaccttgtt ttatatgtaa atattatcca agtacttttc tgattgactc gataacacgg
     2161 tcagcctgaa atggcttaac gatgaaatcc ttggcacctg actggatagc gtcaataacc
     2221 atagactgct gtcccatagc agagcacata acgcatgtag catttgggtc agctgcttta
     2281 atttccttta aagcctggat gccatcctta tttggcatag tgatgtcaag tgttacaaga
     2341 tcaggcttga gctcattgta ctttgcaact gcctcatttc cgtcagcagc ctcgcctgca
     2401 atctcgaatc caccctttgt aaggatatcc ttcagcatca ttctcataaa tgctgcatca
     2461 tcaactatta aaattctagc cattttgtta aacctcctgt ttgataatct gttttactat
     2521 gcttattgga tacaactgta agagatcact gtctattaaa tctccaacag tcagcctgaa
     2581 tgtcaccttg acaatatctg ttccgttaca caactcgtcc ggtatatcaa aggtcttctg
     2641 ataacttgaa ttgataagcg gaaaacctat gtcggttggc tcgtttaaga gcgaagacat
     2701 tgatgtagct attgtaccac acatctggtt catagcctcc gatatggcgg aaagtgtgat
     2761 ttcatcaagt gtcatatcag aaacattact tccgttgcct cccatcatga gattggcaat
     2821 aaccaatgca tcatttaatt ttataataag taggttggtt ccgtcaagtc cttttgtgta
     2881 aggaacctta attaaaattc gatcatcatg atcgagagca tcctcttttt tcaaaacctc
     2941 tacaatcgga gtcgtaatat ttgtcggttt ttgtagaatc atgctcagtg ttgtggctgc
     3001 attgcctatg cagatattgg ctacttcgcc tatgatatcg gcattttccc gagatatatt
     3061 ttgcattatt ttatcctcca tatgactgtg gtacctattt cctaacatat attatatcag
     3121 agtttttaaa aatatcaaag gaaatgttta atttttttga atttatgtta aaatagtttc
     3181 aaaaggagat tacaatatgg gtgaaacaga atattcagaa gcattaaagc tgggaaaaaa
     3241 agaatacagg gcaaggatag ctaaaggaca gtttccttat ctgccagtgc ttgatgaaat
     3301 cttatcagag gcagatattc agacagagca gaatatgggg ctggttcatg tgcctttgga
     3361 tttcgttgta ggaacaagca ccatgggcag gacatattca tttgcggcaa attttatgcc
     3421 gatattggac tgggaaaccg aatttgcagt gaagtggtct aatctgtcag atgcacagat
     3481 gaatgaggga atcagagatc caatcaaggc atttgagtac atgaacaggt attatgtgct
     3541 tgagggcaat aagagggtca gtgtactcaa gtacttcaac gccgtttcga ttcctgcaat
     3601 tgtgaccaga aagattccaa agctgtcgga tgattatgat gtgaggcttt attatgagta
     3661 tatgaagttc aatgaaatca cggggctttg ctcggtggag tttacgaagc tgggcaatgc
     3721 tgataagctt ctttcgcttg ttggaaaaga gggcagatgg gatgatgaga ccaaggaaaa
     3781 atttgccaag gtaatgtttg atttttcaaa ggtctataat ttccgcggtg gtgacaggct
     3841 ggacat
//
LOCUS       DPUF01000235            4381 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_35154, whole
            genome shotgun sequence.
ACCESSION   DPUF01000235 DPUF01000000
VERSION     DPUF01000235.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_35154"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="DHV81_08185"
     CDS             <1..534
                     /locus_tag="DHV81_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI94423.1"
                     /translation="IMMPVMDGIEAMVEIRKISNVPVILLTAKSEDTDKVLGLTVGAD
                     DYVTKPFNPVELQARVKSQIRRYMLLGAGNTSAGKLVIGGIELDDKSKTVTVDGEAAN
                     LTRTEYDILKLLMEHPGEVYSPNQIYAKVWKDEPYGTENTVAVHIRHLREKVEINPAE
                     PRYLKVIWGRGYKIDAE"
     gene            591..3014
                     /locus_tag="DHV81_08190"
     CDS             591..3014
                     /locus_tag="DHV81_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI94424.1"
                     /translation="MKKISRNLWLKLAAFIVCSVMAAGLVVTTIVAVYTNNYESTAQL
                     RDEIHANIAAAYSRQMVYNYESGDDTAFSQMMDDTNLQAAVVKSNETSEDKVDVKSDA
                     AKVYSSVDKISNPDFIFSYKAKNLKWNRLSNVWDAMSGSCQESDDASNDMNSEKISSI
                     MYSKTNGLFYLCLNDGTAYVIPKVRVMTGSNDNSSLGLYKIVEKTDENGNKYFAYEEM
                     FTGQPLDITGFAEWKNVKCYDEKGNHIKCGMNLGDNDNSGFESDGSYYDYDEDIYYDR
                     YEIIKVVEQVNESYELLPDTFNLSNTGFVYYPYTSKGEKPEYYYVYAKLPEQLQEKGD
                     YFNQADSLLDVMEFTEAHLSVLFVIFILGFVITAGYLLTVAGKRAESEEIKLRLYDRL
                     PYEVMLGVTMLADFMVFPAGFLLIKQLPVVALICLAATMVSLEAILSTYVRIRAHEFW
                     KHTLCYYVVRFFLKPVKKLCRMVFAPIRYGFSWIGRNVPLFLISILAFGFICLLELIG
                     FGMELIGFVFIIKLIELALLIAFIMQYDRIRCAAKAIANGDFSQPVDTSRLMLFFRKH
                     GEDLNNVSNGIEAAVSEKMKSERFRTELITNVSHDIKTPLTSIINYVDLLQKEDIDNE
                     KVREYLDVLDRQSSRLKKLIQDLLEASKASTGSINVELEELDAAVMLSQVAGEYKEKF
                     EKNNLDLIIKNDVTPVMIQADGRHLWRVFDNLMNNINKYAQHGTRVYIDIIPKDSGAI
                     ITFKNVSATPLNISSDELKERFVRGDSSRNTEGSGLGLSIAESLMKLMNGTLELTVDG
                     DLFKVTLEF"
     gene            complement(3126..3851)
                     /locus_tag="DHV81_08195"
     CDS             complement(3126..3851)
                     /locus_tag="DHV81_08195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94425.1"
                     /translation="MKILISREQLELLLEKKRDFIGKKITIDTIIAGISFLISVWTAN
                     YETIWIIPGIVFKTIFCVIGIVYMIKIIYDIIDFKNNNYTHTDLLRDIEGLDMIQHNH
                     SLIIIKNSAPGIKTKYLTYYDERWDCKLFPNLKTADKDNEAFIISNLSNDLGIPKKEI
                     KCKYISSRVQEKYSVSHNENRVYNHRLYEVEFKNIPKIMNKNDFSINSRHYYWMTISE
                     MEKDDNIMKKNMEVVDFVKECEK"
     gene            complement(3983..>4381)
                     /locus_tag="DHV81_08200"
     CDS             complement(3983..>4381)
                     /locus_tag="DHV81_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCI94426.1"
                     /translation="LDMFDELKGRIANGLPVLATCAGLILLASHISNDDNVYFGTLPV
                     TVKRNAYGRQLGSFEATARFDNSFDFTMTFIRAPYVEAIDASAKNDVRVLAEYDGKIV
                     GVQYKNQIGLAFHPELDDDTRIHERFLEMI"
BASE COUNT         1358 a          781 c          982 g         1260 t
ORIGIN      
        1 atcatgatgc ctgtcatgga cggcatagag gcgatggtgg aaatcagaaa aatctctaat
       61 gtgccggtca tactgctcac tgccaaaagt gaggacactg acaaggtact tgggctcaca
      121 gtcggagcag acgactatgt gacgaaaccg tttaacccgg tagagctgca ggcaagggtg
      181 aagtcgcaaa tcaggagata catgcttctt ggcgcaggca atacaagcgc aggaaagctg
      241 gtaatcggcg gcattgagct tgatgacaaa tcaaagacag tgacagttga tggcgaggca
      301 gcaaatctca cacgtacaga gtacgatatt ttgaagcttc tcatggagca tccgggcgag
      361 gtgtattcgc cgaatcagat atatgcaaag gtctggaagg atgagccgta tgggacagaa
      421 aacacggtgg cggtacatat ccgccatctc agagaaaaag tggagataaa tccggctgag
      481 ccaagatacc taaaggtcat ctggggccgc ggatacaaaa ttgacgctga ataggtgcat
      541 gcttgataaa gtttgtcaag tgaagcacta taaaatggaa ggtgaatgat atgaaaaaaa
      601 tatcaaggaa tctgtggctt aagcttgcgg catttatagt gtgctcggtc atggcggcag
      661 ggcttgttgt aacaacgata gttgcagtgt atacaaacaa ttatgaaagc acagcacagt
      721 taagggatga gattcatgcg aatattgcag cagcctatag cagacagatg gtgtacaact
      781 atgaatccgg tgatgacact gcattttcgc agatgatgga cgataccaac ctgcaggccg
      841 cagtggtaaa gagcaatgaa acatcagagg acaaggtaga tgtgaaatct gatgctgcaa
      901 aggtgtattc aagcgtagat aaaatatcaa atccggattt tatttttagc tacaaggcaa
      961 agaacttaaa gtggaacagg ctatcaaatg tgtgggatgc aatgagtggc agctgtcaag
     1021 agtcagacga tgcatcaaac gatatgaaca gtgagaaaat cagctcaatc atgtattcaa
     1081 agacaaatgg gctcttttat ctgtgcctga atgatggtac cgcttatgtc attccaaagg
     1141 tgagggtgat gacaggctct aacgataaca gttcgcttgg cttatataag atagttgaga
     1201 aaaccgatga gaatggaaat aaatactttg cctacgagga gatgtttaca ggccagccgc
     1261 tcgatattac aggcttcgcc gagtggaaaa atgtaaagtg ctacgatgaa aaaggaaatc
     1321 atataaagtg tggcatgaat ctgggggata acgataattc gggctttgaa agtgacggct
     1381 cttactacga ttatgatgag gatatttatt atgaccgtta tgaaatcata aaagtggtgg
     1441 agcaggtgaa tgagagctat gagcttttac cggatacatt taatttatca aataccggct
     1501 ttgtgtatta tccatatacc tcaaagggag aaaagccgga gtattattat gtgtatgcaa
     1561 agctgcctga gcagcttcag gaaaagggcg attacttcaa tcaggcagac agcttacttg
     1621 acgtcatgga atttacagag gctcatttga gtgtattgtt tgtcatattt atacttggtt
     1681 ttgtgattac agcggggtat ctgctgacag ttgccggaaa acgtgcagag tcagaggaaa
     1741 ttaaattacg attatatgac aggctgccgt atgaggtgat gcttggagtg acaatgcttg
     1801 cagactttat ggtatttccg gcaggctttt tacttataaa gcagcttcca gtggtggcac
     1861 tcatttgtct ggcggcaacc atggtttcgc ttgaagcaat cctgtcaaca tacgtaagaa
     1921 tcagagcaca tgagttctgg aagcatacat tgtgctacta tgttgtgaga tttttcttaa
     1981 agcctgtcaa aaagctatgc aggatggtat ttgccccaat cagatacggt ttttcatgga
     2041 taggaagaaa tgtaccgctg tttctgatca gtatactggc attcggtttt atatgtctgc
     2101 ttgagcttat aggctttggc atggagctta ttggctttgt atttattata aagcttatag
     2161 agcttgcgct gcttatcgca tttatcatgc agtatgacag gataagatgc gccgcaaagg
     2221 caatagcaaa cggtgatttt tcacagccgg ttgatacgtc aagactcatg ctgtttttca
     2281 gaaagcatgg agaggatttg aataatgtga gcaatggaat agaggcggcg gtctctgaaa
     2341 aaatgaagag cgagcgcttc aggacggagc tcatcacaaa tgtgtcgcat gatatcaaga
     2401 caccgctcac ctcgattatc aattatgtgg acctgttgca gaaggaggac atagataacg
     2461 agaaggtacg cgaatatctt gacgttctcg acagacagtc atcacgtctg aaaaaattaa
     2521 ttcaggattt acttgaggct tcaaaggcat ctaccggcag cataaatgta gaacttgagg
     2581 aactggatgc ggctgttatg ctgtcacagg tagccggaga gtacaaagaa aaattcgaga
     2641 aaaataatct tgatttaatt atcaaaaatg acgttacgcc tgtcatgatt caggcagacg
     2701 gacgacatct gtggagagtg tttgataacc tgatgaacaa tatcaacaaa tacgcccagc
     2761 atggcacgag agtgtacatt gacatcattc caaaggacag cggagccatc ataacattca
     2821 agaatgtatc agctacaccg cttaatatct caagcgatga gcttaaggag cgttttgtca
     2881 gaggagacag ctcgcgaaat accgagggca gcggactggg actgtctatt gcagaaagcc
     2941 ttatgaagct tatgaatggt actttggagc tgactgtgga cggtgatctg tttaaggtga
     3001 ctttggagtt ttaggctttt taaaacaata ttatgatacc tattgaccgt ctaatcatat
     3061 tatatattga acttataaga cataagaaac aacaaaagcc ggggcagatg ccccggctta
     3121 aaaaattatt tttcgcattc ttttacaaaa tcaactactt ccatattctt tttcattata
     3181 ttatcgtctt tttccatttc actaattgtc atccaataat agtgtcggga gttaatagaa
     3241 aagtcgtttt tattcattat ttttggaata tttttaaatt ctacttcgta cagcctatgg
     3301 ttatacactc tgttttcgtt atggcttacg gaatattttt cctgtactct ggatgatata
     3361 tatttgcatt tgatttcttt tttgggtata cctaagtcat ttgataaatt actgatgata
     3421 aaggcttcat tgtctttatc tgcggttttt aaattgggaa acagcttaca gtcccatctt
     3481 tcatcataat atgtaagata ttttgttttt atgccggggg cactgttttt gattataatt
     3541 aatgaatgat tatgctgtat catgtccaat ccttctatat ctctcaataa gtctgtatga
     3601 gtgtaattat tgtttttgaa atcaataata tcatatatta tttttatcat gtaaacaatt
     3661 ccgataacac aaaatatagt tttaaataca attcccggga tgatccatat ggtttcatag
     3721 tttgctgtcc atactgatat taagaaagat atacctgcta taattgtatc tattgttatc
     3781 tttttgccta taaaatcgcg ctttttttca agtaaaagct ccaattgttc cctactaatt
     3841 aaaattttca taacccctcc ggataatagt gttgaaacac atgatatata attataaatg
     3901 atgaaaaatg atatggcaat aaaaatataa atagaaaaca agaaggaatg ctattacaaa
     3961 ttataataac attcctgaat aatcatatca tctccaaaaa cctctcatga atccgtgtat
     4021 cgtcatcaag ctccggatga aaagccagcc caatctggtt cttatactgc acaccgacaa
     4081 ttttgccgtc atactctgcg agtacacgca catcattctt tgctgaagca tcaatagctt
     4141 ccacgtaagg cgccctgata aatgtcatgg taaagtcaaa gctattgtca aaccttgctg
     4201 tagcctcaaa gcttccaagc tgtctgccgt aggcatttct tttaacagtg acaggcaaag
     4261 tgccgaaata tacgttgtca tcgttgctga tatgtgaagc taaaaggata agaccggcgc
     4321 aggttgcaag caccggaagt ccgtttgcga ttcttccctt aagctcatca aacatatcaa
     4381 g
//
LOCUS       DPUF01000236            3406 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_41537, whole
            genome shotgun sequence.
ACCESSION   DPUF01000236 DPUF01000000
VERSION     DPUF01000236.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_41537"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="DHV81_08205"
     CDS             complement(<1..504)
                     /locus_tag="DHV81_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HCI94427.1"
                     /translation="MQIVDILKAAQQQNSSDIHIVPGNPVMLRIDGKLVPQGNQVLTN
                     VETDNLLSPIVPQELKDELKEKGELDFAFSVEGFSRVRANVFRQRGSYAAALRILSYD
                     VPTPQQLGIPQSVVNLTTKMRGLVLVTGATGSGKSTTLASLIDVIASRDSKNIITLED
                     PIEYLHSR"
     gene            complement(518..2221)
                     /locus_tag="DHV81_08210"
     CDS             complement(518..2221)
                     /locus_tag="DHV81_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCI94428.1"
                     /translation="MAFRREKKRIGDMLINENVITQEQLEKALPIAKEKHKKIGETLI
                     ELGFTNELEIAKALSQQLGLELVNVSAINIPEEVQNLVSETVLRKHVMIPYAFDKNNA
                     NVVHVAMADPMDMVALDDFSIVTNLQVEPAVATGRDILLTLDKYYGDTEAMKAAQEYA
                     RERKEREQKNAEAEEATSKDVNNSPVVLLVNSIIEQAARLRASDIHIEALENKVRVRY
                     RIDGALYEKAAYSIHLLSAIITRLKIIGGMDISEKRKPQDGRITMEIDKIEYDIRVSI
                     LPTVFGEKCVMRLAQKKALTRDKKELGFSDEELKAFDHILMNTNGIILVTGPTGSGKS
                     TTLYTALSELNKEDVNIITVEDPVEANIDGINQVQVNNKADLTFASALRSILRQDPDI
                     IMIGEIRDQETAQIAVQASITGHLVVSTLHTNSSASTISRLEDMGVESYLLADSVKGI
                     IAQRLVRRLCPACKREHLLTEEEKAFMNIPAFRPVKIYEPCGCEKCANTGYKGRIGIY
                     EIMTITPKIKSLISKGVDIEDINKAACDEGMHTLRQSAEKLVLEGVTSFQEMLKTTFE
                     N"
     gene            complement(2321..>3406)
                     /locus_tag="DHV81_08215"
     CDS             complement(2321..>3406)
                     /locus_tag="DHV81_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCI94429.1"
                     /translation="TIGGWVLKYFTLFITGSGKNAVADGYFTGFITGQWAPIIFGVVY
                     LLITAAVVIGGVNKGIERFSKVLMPILVVLIFAIGIFSLTLNYKDASGAARSGLDGLK
                     IYVVPDFKGLTMQKLVTVFVDALGQLFYSISVAMGIMVAYGSYVKKESKLMGSINQIE
                     IFDTLVAFLAGLMIIPAVYVFMGRDGMSAGPGLMFISLPKVFNEMGIAGDIVGLIFFM
                     IVAFAAVTSSVSIMEAIVSSLIDRFHWSRRKSAILVTVYGLIAEIIVCLGYNKLYMEV
                     KLPNGTVGQILDIMDYVSNNVLMPIVALATCILVGWIVSPKVIIDEVTRGGSKFTRKG
                     LYIIMVKFIAPAFLLILLLQALGIVTF"
BASE COUNT          902 a          836 c          624 g         1044 t
ORIGIN      
        1 ccttgagtgc aggtactcga taggatcttc aagcgtgatt atatttttgg aatctcttga
       61 tgcaatgaca tctataagac ttgccaatgt ggtcgactta ccacttcctg tagctcctgt
      121 cacgaggaca agtcctctca tctttgtggt aagattgaca accgactgtg gtatgccaag
      181 ctgctgcggt gtaggcacat catatgagag aatacgaagt gctgctgcat atgaacctct
      241 ctgtctgaac acattggctc tgactcgtga aaagccctcc accgaaaaag caaaatcaag
      301 ctctcccttc tcctttagct catctttaag ctcctgcgga acaattggag aaagcaggtt
      361 gtccgtctca acattggtaa gaacctgatt tccctgcggt acaagctttc cgtcaattct
      421 aagcataacc ggatttcccg gtacgatatg tatatccgat gagttctgtt gctgcgctgc
      481 ttttaaaatg tctactatct gcattttctt ttccccctta gttttcaaac gtggtcttta
      541 acatttcctg gaatgaagta actccttcga gtacaagctt ctctgcactc tgtctaagtg
      601 tatgcatgcc ctcatcacat gcagccttgt ttatatcctc tatatcaaca ccttttgaaa
      661 taagtgactt tatctttggt gttatggtca ttatctcata tataccgatt cgtcccttgt
      721 agcctgtatt ggcacatttt tcacagccac atggctcata aatctttacc ggacggaatg
      781 ccggtatatt cataaaggcc ttttcctcct ctgtcaacag atgctctctc ttacatgccg
      841 gacaaagtct tcttacaagt cgctgtgcaa tgattccctt taccgaatcc gcaagcagat
      901 aactttcaac tcccatgtcc tccagtcgtg aaatagtact tgccgagctg ttggtatgca
      961 gtgtgctgac cacaagatgt cctgtgattg acgcctgtac tgcaatctgt gctgtctcct
     1021 gatctcgaat ctcaccaatc ataatgatgt ccggatcctg tcgcagaatg gaacgaagtg
     1081 ctgatgcaaa tgtcaggtca gctttattat ttacctgtac ctggtttatt ccatctatat
     1141 tggcctcgac cggatcctca actgtgatga tatttacatc ctccttgttg agctctgaaa
     1201 gtgccgtgta aagcgtggtt gacttaccac ttcctgtagg tcctgtaaca aggatgatac
     1261 cgtttgtatt catcagtata tgatcaaagg ccttaagctc ttcgtctgaa aatcccagct
     1321 cctttttatc tcttgtaagt gcctttttct gcgcaagtcg cataacgcat ttctcaccga
     1381 atactgtcgg aaggattgat actcggatat catactctat cttgtctatc tccatagtga
     1441 tacggccgtc ctgcggctta cgtttctcgg aaatatccat accacctata attttaagtc
     1501 gtgtaataat tgcagaaagc agatgtatac tatatgctgc cttctcataa agcgcaccat
     1561 ctattctgta tcgcaccctg actttatttt ccagtgcctc tatatgtata tctgaggccc
     1621 tgagtctggc tgcctgctca atgatagagt tgacaagtaa aactaccggc gaattgttga
     1681 catccttgct ggtagcttcc tcagcctcgg catttttctg ctcacgctct tttctctccc
     1741 tcgcatactc ctgagccgct ttcatagcct ctgtatcacc gtaatatttg tcaagcgtca
     1801 aaagtatgtc tctgcctgtc gctactgcag gctcaacctg tagattggta acaattgaaa
     1861 aatcatcaag cgcaaccata tccataggat cagccatggc cacatgcact acattggcat
     1921 tattcttgtc aaatgcatat ggtatcataa catgctttct aagcactgtc tcactgacaa
     1981 ggttttgcac ctcctcaggt atgttgattg cgctcacatt aacaagctcc agtcccagct
     2041 gctgtgacaa tgcctttgct atctcaagct cattggtgaa cccaagctca ataagtgttt
     2101 ctcctatctt cttatgcttt tctttggcta taggcagagc cttttccagc tgctcctgag
     2161 taattacatt ttcatttata agcatatcac cgatacgctt tttctctcgt ctaaaagcca
     2221 tagtggctcc ccctctttca tcatttagta aaatactacc attatgatgg ctgtatttca
     2281 agggtattta tataaaatat acaataaatt tatttcaaaa ctaaaaagta acgattccga
     2341 gtgcctgcag caacagtata agcaggaatg ccggtgcaat aaatttaacc atgattatat
     2401 aaagcccttt tctggtgaat ttgctgcctc cgcgtgtcac ctcatctatt attacctttg
     2461 gacttactat ccagccaacg aggatacatg tggcaagtgc aacgattggc ataagcacat
     2521 tgttgcttac ataatccatg atatcaagta tctgtccgac tgtgccgttt ggaagcttaa
     2581 cctccatata gagcttattg tagccaaggc agactattat ctctgcaatc agaccgtata
     2641 ccgtgactaa aattgcgctt tttctgcggc tccagtgaaa tctgtctata agactcgata
     2701 caatagcctc cataattgat acagacgatg tgactgctgc gaatgcaact atcatgaaga
     2761 atatcagtcc aacgatatct cctgctattc ccatttcatt aaataccttt ggcagtgata
     2821 taaacataag ccccggtccg gctgacatgc cgtcacggcc cataaataca tatactgccg
     2881 gaataatcat aagacctgca aggaatgcaa caagtgtatc aaaaatctca atctggttga
     2941 ttgagcccat gagctttgat tccttcttga catacgaacc atatgcaacc atgattccca
     3001 tagccacact gattgagtag aacagctgac cgagcgcatc aacaaatact gtgacaagct
     3061 tttgcattgt aagccccttg aagtcaggaa ccacatatat cttaagacca tcaagaccac
     3121 ttcttgctgc gcctgatgca tccttataat tgagtgtcag tgagaaaata ccgattgcaa
     3181 aaataagcac aacaaggata ggcatgagca ccttgctgaa gcgctcgata cctttattga
     3241 ctccgccaat gacaactgct gctgttataa gcagataaac cactccaaat atgattggtg
     3301 cccactggcc tgtgataaag cctgtgaaat atccatccgc aaccgcattt ttaccgcttc
     3361 ctgtaatgaa aagtgtaaag tattttaaaa cccatccacc tatagt
//
LOCUS       DPUF01000237            5207 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13226, whole
            genome shotgun sequence.
ACCESSION   DPUF01000237 DPUF01000000
VERSION     DPUF01000237.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13226"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            78..1577
                     /locus_tag="DHV81_08220"
     CDS             78..1577
                     /locus_tag="DHV81_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94430.1"
                     /translation="MKTIKRGITLVLAMILTFAMSVTVFADGESAKKTFTITVNNAKA
                     GHTYEAYQILKGDLSESETTLSNVGWGSGIQEDKQTDLESNKDAKAYVEKLSGMKSNS
                     SELKAEAQKIASALSTTVAGSVSVTQDNAKTEITGLEPGYYLIKDKDSSLTGDEAYTE
                     YILNIVADTTITPKTDVPSVEKKVKDINDTTGDTTGWQDSADYDITDVVPFQLTATLP
                     ANVESYKSYYLKFDDTLSEGLDFNEGTVTVKLGDKDVTSFFTTNYDAAGKKLTFTCDN
                     ILAEGFEAKNGDKVVVEYNATLNENAVIGSKGNPNKVKLEFSNNPNNGGEGDKGETPE
                     DTVIVFTYKVVINKVDENNKALDGAEFTLYKKIKGEADKEIKAVISGDKNDVFTFSGL
                     DDGDYVLKETKTPDEYNTANDIAFTIKADHSIESDAPELNSLSGDKVTGNITLKADKA
                     EGSLSTPVQNLKGSVLPSTGGAGRVAIYVIGAILVVGGGIVLVTKKRVK"
     gene            1681..2502
                     /locus_tag="DHV81_08225"
     CDS             1681..2502
                     /locus_tag="DHV81_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCI94431.1"
                     /translation="MRRKLSTILFVIVFLAGLSLLLYPTVSDYWNSFHQSRAIASYVD
                     AVDNTDEQKLEEMRQAAEAYNEKLLSKQDRYDMSDSDRAEYESLLDVSGTGVMGYVEI
                     PSINVSLPIYHGTDNTILQIGVGHIEGTSLPVGGASTHCAVSGHRGLTSSKLFTDIDQ
                     MVEGDTFKLYVLGETLTYEVDQIRIVLPDELNDLKIEEGQDYCTLITCTPYGVNSHRL
                     LIRGHRIANDAQGLIVEDVKQVKPLHEAIVLGILFLVLYIIICKILSKTILYRFR"
     gene            2521..3474
                     /locus_tag="DHV81_08230"
     CDS             2521..3474
                     /locus_tag="DHV81_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94432.1"
                     /translation="MHNSIKRAVSIVIAAIVLFSLPCGSIFARTHIDIDKKCSITVDI
                     PDTWEDLYTVDFPVELYRVADVDENDVYTATKQFAELAKSISDISSKTTADDWEKMAK
                     TAADIADKGSLTADEKIDVSNGRGTVTGVKTGLYLVYAKPANSARFGYTFVPYLVSAP
                     NNEFAMTGSGSDSWIYDDIDIELKPEQTGLMGSLQINKTLKTYNTALGEPVFAFDVEA
                     YDRDGNVVFSDIASIRFDSTGAKSVIIDNIPAGSRVIVKEVYAAGSYQVTSSDSIETQ
                     IVAGETADVDFTNDYNDKLIYSTGVVNHFEYDGTGWEWVQN"
     gene            3459..4016
                     /locus_tag="DHV81_08235"
     CDS             3459..4016
                     /locus_tag="DHV81_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94433.1"
                     /translation="MGAELMKKLSESCKKLNRNHRILVCAVFAATLIVCGVSVKPTLA
                     YFTTYATAKGGISVDIGPTTDVKEKFKDWKKTIEIENTGKADCFVRVKVIAASQFDIQ
                     ASGSNWSLSDDGYWYYSQVVPVGGKTEPIVAAITVSEKVKTSFNVVVVQECTPVIYDE
                     NGNPCVAPDAKWDATAQYDKEEGEE"
     gene            4016..4780
                     /locus_tag="DHV81_08240"
                     /pseudo
     CDS             4016..4780
                     /locus_tag="DHV81_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742701.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    4540..4678
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4792..>5207
                     /locus_tag="DHV81_08245"
     CDS             4792..>5207
                     /locus_tag="DHV81_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94434.1"
                     /translation="MKKFIALLSCMLFILGSIGTVFADENTTTAGSCEFTGKEMVSSF
                     DSNKIAASVSQMEPGDSVTFSVDIKNNSSISTKWYMSNDVLKSLEDTQAVAENGGYSY
                     ILTFVGPDGKEDTIYSSTSVGGEKEATAGKGLNEAT"
BASE COUNT         1652 a          866 c         1212 g         1338 t
ORIGIN      
        1 gagttgtgct tatagcaata gcttcaatta cacttttcta taaaaacata aaacattata
       61 aaaaaggaga atgcaatatg aaaacaatca aaagaggcat tactttagtt cttgcgatga
      121 tacttacatt tgcaatgagc gttacagtat ttgctgatgg cgaaagcgca aaaaaaacat
      181 ttaccatcac ggtcaataat gcaaaggcag gccacactta tgaggcttac cagattttaa
      241 agggtgatct ttcagaatca gagacaacac tttcaaacgt tggctggggc agcggtatcc
      301 aggaagacaa acagacagat cttgagtcta ataaggatgc aaaagcttat gtagagaagc
      361 tttcaggaat gaagtcaaac tcttcagaac ttaaggcaga ggcacaaaaa attgcttcag
      421 ctttaagcac tacagtagca ggttctgttt cagtaacaca ggacaatgcc aagactgaga
      481 ttacaggact tgagcctggt tactatctca tcaaggataa ggatagctct ttaacaggtg
      541 acgaggctta cacagagtat atcctcaata tcgttgctga tacaacaatc acaccaaaga
      601 cagatgtacc ttctgttgag aagaaagtaa aagatataaa tgacacaaca ggtgatacaa
      661 ccggatggca ggattcagcc gattatgata tcacagatgt ggttccattc cagcttacag
      721 ctacattacc tgcaaatgtt gagtcatata agtcatatta cctcaaattt gatgatactc
      781 tttcagaggg acttgatttc aatgagggaa ctgttactgt taaattaggt gataaggatg
      841 ttacatcatt ctttacaaca aattatgacg cagctggcaa aaagcttact tttacatgtg
      901 acaacatttt agcagaggga tttgaagcta aaaatggtga taaggttgta gttgagtata
      961 atgcaactct caatgagaat gctgttatcg gctcaaaagg aaacccaaat aaggttaagc
     1021 ttgaattctc taacaaccct aacaatggcg gagagggaga caaaggtgag actccggaag
     1081 atacagtaat cgtatttaca tacaaggttg taatcaacaa ggtagatgag aacaataaag
     1141 cactcgatgg tgcagagttc actctttata aaaagattaa aggcgaagca gacaaagaaa
     1201 tcaaagcggt aatttccggt gataagaatg atgtcttcac attcagcggt cttgatgatg
     1261 gtgattatgt acttaaggag acaaagactc ctgatgaata caatactgca aatgatattg
     1321 cattcacaat caaagcagac cactcaattg agtctgatgc tcctgagctt aattcattaa
     1381 gcggagataa ggtgaccggt aatattacac ttaaagcaga taaagctgag ggtagcttaa
     1441 gcacacctgt acagaactta aagggctcag tccttccttc aaccggtggt gccggacgtg
     1501 ttgcaattta tgtaatcggt gctatattag ttgtaggtgg cggaattgtt cttgtaacaa
     1561 agaagcgtgt taaataatat cattttatga taaaatatat ctgccatgag ccactttagt
     1621 ggttcgtggc agttacaata ggcataatta tatgcaaatg ataacagtaa ggagagtgct
     1681 atgagaagaa aattatcaac tattttgttt gtcatagtgt ttcttgcagg actgtcctta
     1741 ctgctatatc cgacggtgag tgattactgg aattcattcc atcagtcaag ggcaattgca
     1801 agctatgtcg atgcagtaga caatacagat gaacagaagc ttgaagaaat gagacaggcg
     1861 gcagaggctt acaatgaaaa gctattaagc aagcaggaca gatatgatat gtctgattca
     1921 gacagagcag agtatgaaag tcttcttgat gtatcaggca caggagtcat gggctatgtt
     1981 gagattccgt ccatcaatgt ctcattgccg atttaccacg gcacagacaa tacaatactt
     2041 cagataggtg ttggccatat cgagggcaca tcgcttccgg ttggcggagc aagcacacat
     2101 tgtgctgttt caggacacag aggacttacg tcatctaagc tttttacgga tattgaccag
     2161 atggtggagg gtgacacctt caagctgtat gtgcttggcg agacactgac ctacgaggtt
     2221 gatcagataa ggattgtttt accagatgag cttaatgact taaagattga ggaaggtcag
     2281 gattactgta ctcttatcac atgtacacca tatggtgtga attcccacag gcttcttatc
     2341 agaggtcaca ggattgcaaa tgatgcacag ggacttattg tcgaggatgt aaagcaggta
     2401 aagccgttac atgaagccat agtgcttggc attctgtttc tggttttgta tattataatt
     2461 tgtaagatac ttagtaaaac gatattgtat agattcagat agaatcagga aggaggaaaa
     2521 atgcacaata gtataaaaag agctgtcagt atagttatag cagcgattgt cttgttttcc
     2581 ctgccatgtg gaagcatttt tgcaaggacg catattgata ttgataagaa atgctctata
     2641 acagttgata tccctgatac atgggaggat ctgtacactg tggattttcc cgtagagctt
     2701 tacagagtgg cagatgtaga tgagaatgat gtatacactg caacgaagca gtttgctgag
     2761 ctggctaaat ctatatcaga tatcagcagc aaaactaccg cagatgactg ggaaaagatg
     2821 gcaaagactg ctgcggacat tgcagacaaa ggttctctga cagcagatga gaaaatagat
     2881 gtttcaaacg gaaggggaac cgtcacaggt gttaagacgg gactgtatct ggtgtatgca
     2941 aagccggcta attcggcaag atttggatac acatttgtgc cgtatctggt ttcggctcct
     3001 aataatgagt ttgccatgac aggaagcggc tcagattcat ggatatatga tgatatcgat
     3061 atcgagctga agcctgaaca gaccgggctt atgggaagct tacagattaa caagacatta
     3121 aagacataca atacagcact cggtgagccg gtgtttgcat ttgatgttga ggcctacgac
     3181 agggatggca atgtggtgtt ttccgatatc gcatcaatca gatttgacag tacaggagct
     3241 aagagcgtaa ttattgacaa tattccggca gggtcaaggg tgattgtaaa ggaggtatat
     3301 gccgcaggca gttatcaggt gacctcctca gacagcatag agacacagat tgtggccggt
     3361 gagaccgcag atgttgattt cacaaacgat tacaacgaca aattgattta tagtaccggt
     3421 gtcgtaaacc attttgagta tgacggaacc ggatgggaat gggtgcagaa ttaatgaaaa
     3481 aattatcaga gtcttgtaaa aaacttaata gaaatcatcg tattcttgta tgtgcagtgt
     3541 ttgctgctac actcattgtc tgtggtgttt cagttaaacc gacactggca tattttacaa
     3601 cctatgctac ggcaaagggc ggtatctcag tagatatcgg tcctacaact gatgtcaagg
     3661 agaagtttaa ggactggaag aaaactattg agattgagaa tacaggaaaa gcagactgtt
     3721 ttgtcagggt aaaggtaatt gcagcaagcc agtttgatat acaggcatca ggctcaaact
     3781 ggagcctttc agatgatggc tactggtatt acagccaggt agtgccggtt ggtggcaaaa
     3841 ccgagccaat tgttgcagct atcacagtta gtgaaaaggt aaagaccagc ttcaatgtag
     3901 ttgtggttca ggagtgtact cctgtcatat atgatgagaa tggcaatccg tgtgtcgcac
     3961 ctgatgcaaa atgggatgcg acagcacaat atgataaaga ggagggcgaa gagtaatgaa
     4021 gagaaaactt ccaattatac ttgtagcatc ctctgcgata atgcttgcag catcgacgat
     4081 tggatctaca aaagcgactc ttacatatat gagtgataat tatctggctc agattgacat
     4141 caaatccatc ggtgttactt tgactgagaa tggcaatgat gtgagctggc gtgattataa
     4201 gcataaggat gatgactgga gcgaggcaac aggagtgctg cttggagata tgctaaagaa
     4261 tgccggagat gaaaaactga ttctcgacaa aaagtacgat gagaagcttg ctgtcaaaaa
     4321 cagcggctcg atagatgagt atgtgagagt gactgtgcag cattactggg cagacaagga
     4381 gacaggtgat aagctttcaa aacttgatcc atcaaagata cagattgact ttacaaacga
     4441 tggctggaca gaggatgaga gtgctgagac tacagagcga aatgtatttt actacaattc
     4501 tattttaaaa aaaggtcaga catccactga ttttacagan nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
     4681 tgttgatgca gtacagacac acaatgcaaa ggacgcaatt aagagtgcgt ggggtgttga
     4741 tgtaaatata tcagatgacg gggcacttag cttagagtaa aggaggacaa tatgaaaaaa
     4801 tttatcgcat tattatcatg catgttgttt atccttggtt cgataggcac tgtatttgca
     4861 gatgagaata ctactacagc cggctcatgt gagtttaccg gcaaggagat ggtcagttcg
     4921 ttcgattcaa ataaaatagc agcctctgtc tcacagatgg agcccggaga ttctgtaaca
     4981 ttttccgttg atataaagaa taacagcagt atttcgacga aatggtatat gtccaatgat
     5041 gtattaaaga gtcttgagga tacacaggct gtggctgaaa atggtggata ctcatatatt
     5101 cttactttcg taggaccgga tggaaaagag gacactatat acagcagcac cagtgtaggt
     5161 ggagagaagg aggcaacagc cggaaaagga ctcaatgagg ctacaaa
//
LOCUS       DPUF01000238            3089 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_15086, whole
            genome shotgun sequence.
ACCESSION   DPUF01000238 DPUF01000000
VERSION     DPUF01000238.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_15086"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(1..964)
                     /locus_tag="DHV81_08250"
                     /pseudo
     CDS             complement(1..964)
                     /locus_tag="DHV81_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884946.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     assembly_gap    765..860
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(964..2046)
                     /locus_tag="DHV81_08255"
                     /pseudo
     CDS             complement(964..2046)
                     /locus_tag="DHV81_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515722.1"
                     /note="catalyzes production of carbamoyl phosphate from
                     bicarbonate and glutamine in pyrimidine and arginine
                     biosynthesis pathways; forms an octamer composed of four
                     CarAB dimers; too many ambiguous residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
     assembly_gap    1753..1840
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2326..2922)
                     /locus_tag="DHV81_08260"
     CDS             complement(2326..2922)
                     /locus_tag="DHV81_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCI94435.1"
                     /translation="MKIRKLTNEEIKGACAFAVSIYNIAIRGCFRTQESHRYFDEYMD
                     ADRLTDEERAGVLVVFGAFDSNVLCGVCGMTNEGHITMLYVHPQYLRRGIGKKLLERV
                     RIYARMQLKLMQVSVNAMPAYTADYFSRVGFKSAYQGGFCNGQGDMYVPMTAKSIYQV
                     EYTPRHIADKTFIAISVGFTCLVFFSGLIYAVCAGLTP"
     gene            complement(2922..>3089)
                     /locus_tag="DHV81_08265"
     CDS             complement(2922..>3089)
                     /locus_tag="DHV81_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V"
                     /protein_id="HCI94436.1"
                     /translation="PRMLMNRNLLYTAVTRAKKCVTIVGDEQTFYEMIQNNSQQRRYS
                     GLRDRIVEETI"
BASE COUNT          736 a          704 c          563 g          902 t
ORIGIN      
        1 gtaaccttgg cgataggata tcccgtagcc tttgatgcga gtgctgatga acgtgaaact
       61 cgtgggttta cctcgataac acagtactcg aatgagtcag gattgagggc atactgtacg
      121 ttacagcctc ctgtaatctg aagctcgttg atgatattga gcgctgatgt acgaagcatc
      181 tgatactctt tatctccaag tgtctgcgaa ggtgctacta cgattgaatc tcctgtatgc
      241 acaccgacag ggtcgatatt ttccatgtta catactgtga tgcagttgcc gttggcatca
      301 cgcattacct cgtactcgat ttccttccat cctgcgatac atctctctac aagaacctct
      361 ccgacacgtg acagacgaag tccatttgaa agtatgtcta taagctcctg ctcatcgtga
      421 gcaattccac cgccggaacc gccgagtgta aatgcaggac gaagaactac agggtaacca
      481 atcttgttgg taaatgcaat accgtcctcg atattcttta caacctgtga agccgcgatt
      541 ggttcaccga ttttctccat ggtatccttg aaggcctgac ggtcctcagc cttgaagatg
      601 gtctctgctg ttgttccgat aagctttact ccctgctcat caaggaagcc gcactcagcg
      661 agctccatgc cgagattgag tcctgcctgt ccaccgagtg taggaaggat gctgtcaggc
      721 ttttctttta tgataatctc ttttaatgac tcaagagtaa gtggnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn cggcaggcct gtgttcctgc atagtcaaac tctgcggcct
      901 gtccgattac gattggaccg gaaccgataa ctaatacttt tttaatatca ttatttcttg
      961 gcattactgg ttacctccca tcatgttaat aaagcggtca aaaagatatg ctgaatcctg
     1021 tggtccaggg catgcctccg gatggaactg aactgtgaag atattcttgt tcacatactt
     1081 aagtccctca ttagtgccgt cgttgacatt tataaatgca ggctcagcca cattagggtc
     1141 aagcttatct gtgtcaacca catatccatg gttctgggaa gaaatataaa cacgacctgt
     1201 tgcaagatcc ttaaccggat ggtttccacc tctgtgtccg tacttcatct tgtgtgtatc
     1261 tgcgcctgtt gcaagtgcca tgagctgatg tccgaggcag attgcaaaaa tcggcacatt
     1321 gctgtcataa agcttcttta tttcctttat aatatctaca cactcctttg gatctccagg
     1381 tccgtttgag agcatgattc cgtcaggatt gtcaccaagt atctcttcag cggttgtgtg
     1441 tgcagggtaa attgtaactt cacagcctct ttcattgagt gatttggcga tgttgttttt
     1501 ggcaccgaaa tccatgaggg caaccctctt tccttggccc tttaaaacct ttttctcagt
     1561 gcatgtgacc ttgtcaacca cattgcctgt agtatatgcc ttgagctttg gaataatctc
     1621 atcgagattg taattctcat ttgtggtaat cattccgttc atagtaccct tttccctgag
     1681 aatctttgta agcgcacgtg tatcaatgcc ggccactccc ggaatatcgt ttttctcgag
     1741 gaaatcctgt atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gactccatat ccggtgtgat
     1861 tccataattt ccgataagtg gataagtcat tacaacagcc tgtcctgcat aggaaggatc
     1921 tgtaagtacc tccagatatc ctgtcattga tgtgttaaat actatctcac taatgacttc
     1981 ccttgttgaa ccaatgctgg ttccggtaaa tacattacca tcttcaagaa ttaaaaatgc
     2041 tttcatcttt accctgccct ttcgtgttat actttgtaac cataagcttc ttagctgcaa
     2101 ctaaccttac gtctgcgcaa aaaaaaagag ggcgtctctt ttttcttata aagtgtagtt
     2161 gcatagtata tctgcttaaa ttgtaaaaag tgtatcataa tacagccgat ttttcaactg
     2221 ttttttgtta cataaatatg catatttatg tatacaatat tagttactgt tcacaccaaa
     2281 gtatgaccgg caactattgt agcccattaa aactgcatct gagcgttacg gagtaagccc
     2341 cgcgcatacc gcatatatca gtcctgaaaa aaatacaagg catgtaaagc caactgaaat
     2401 tgcaataaaa gtcttatcag ctatatgtcg cggtgtatac tccacctgat atatgctttt
     2461 tgctgtcata ggaacgtaca tatcgccttg tccattgcaa aagcctccct gatatgcact
     2521 cttaaagccc acacgactga agtagtctgc cgtgtatgcc ggcattgcat ttacagacac
     2581 ctgcatgagc ttaagctgca tgcgcgcata tatcctgact ctctccagaa gctttttgcc
     2641 aattccacgt cttaaatact gcggatgcac atacagcatg gtgatatgcc cctcgtttgt
     2701 catgccgcac actccgcaca gcacattgct atcaaacgca ccaaatacta caagaacacc
     2761 ggccctttcc tcgtccgtca gcctgtctgc atccatatat tcatcaaaat atcgatgact
     2821 ctcctgtgtc cgaaagcagc ctctgattgc gatattgtat atacttaccg caaaggcaca
     2881 tgcccctttt atctcttcat ttgtaagttt tcttattttc atcagatagt ctcctccact
     2941 attctgtctc taagcccact atagcgtctc tgctgtgagt tgttttgtat catctcgtaa
     3001 aatgtctgtt cgtcacccac tatggtaaca catttctttg ctcttgtaac cgccgtataa
     3061 agcaaatttc tgttcataag cattctcgg
//
LOCUS       DPUF01000239            4622 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_41842, whole
            genome shotgun sequence.
ACCESSION   DPUF01000239 DPUF01000000
VERSION     DPUF01000239.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_41842"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="DHV81_08270"
     CDS             <1..126
                     /locus_tag="DHV81_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94437.1"
                     /translation="IKTGAPSRSERVAKYNQLLRIEEELGKSAVYPGIKAFNVQQ"
     gene            476..2554
                     /locus_tag="DHV81_08275"
     CDS             476..2554
                     /locus_tag="DHV81_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HCI94438.1"
                     /translation="MDNNNIGGMNPQQFSQNTPQTSQPHMGMSGIELQNMQQEAEQRR
                     REQSRRNADFFGRLCIPTIIYALLYTIFLYENTGGILVTLFAIVTGVYSLYCMKILHI
                     EAKPLTVWYSVMMILTGISSGLTGNKIIQGFNFCWILVFLVFMLLHNFCNDRQWGLIK
                     YIAAAFQAVFGAIGCIAEPFMDIADYMRNERMDSDNMGSDSMVGDSANATAGERHVKK
                     HRMLYVFIGIAIAFPLVVLIVVLLCSADAVFASVIKKIFADINLFTVSKVVFLFVFAL
                     FSSYCGIKYLSKKRISDAPVETPAFPAAIGITVAATISVVYVFFCFIQIVYLFGGLMQ
                     LPSGYTYARYAREGFFQLLFVCILNVIIVLLGSELFRKNKILNAFLILITLCTYIMIA
                     SSAYRMGLYVSEYGLTATRLCVFWALGVIALFMLGVILSICKPAFSLFRYGIIVIGVC
                     YLVLAFARPDYLVARYNTVCMEDTDYKYLMSLSTDASPALAADADFMENKGMVTMYAR
                     QLAGETNDSLRQLNVSHIKAAHLFRDSIDEVKSSQLILLYVYSPYDSGSYNNNDTGLD
                     GVDSIQMGYHVLKDTEDDDTANYDYDSYSMDDTRVAAPVFFKWVDAVEVKKISDSERI
                     FLAKIPRKALKGKDGVNIEYRFNKNGDVIYSSQYNVILDKKKGLNEVELSYYAGTDGV
                     DVPEYNIYGK"
     gene            2665..4260
                     /locus_tag="DHV81_08280"
     CDS             2665..4260
                     /locus_tag="DHV81_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI94439.1"
                     /translation="MGRFLNPGNKAFQKTLKSEIYVDKTMLLAYTNKVIGSDMACICN
                     SRPRRFGKSITANMLAAYYSRGCDSFDMFSTLKVGRLDSFETNLNKYDVIHIDVQWCM
                     MDAGEVQNTVKYINNGILDELIIAYGDVIPDTVKTAYGAMSYINAATGNTFVIIIDEW
                     DVLIRDEANNKELQEEYINFLRGMFKGTEPTKYIALAYLTGILPIKKLKTQSALNNFE
                     EFTMLDAGALASYIGFTDDEVKQLCKKYDRDYEAVKNWYDGYMLSGKHVYNPKAVVSV
                     MMRGSFQSYWSQTGTYESLIPLIDMDFDGLRAAIISMISGNEIKVRTTTFQNDMVSFK
                     NKDDVLTLLIHLGYLAFNQKNQMAYIPNEELRNELMDAVEENKWDEIIQFERQSIDLF
                     NATINKDVNTVAAKIEQIHMEYTSVIQYNDENSLSSVLAIAYLGTMNYYFKPVRELPT
                     GRGFADFVYIPKPEYINDYPALVVELKWNKNADTAMQQIRERQYPNSLLQYTGNILLV
                     AINYDEKTKEHVCKIEEYAKIQN"
     gene            complement(4392..>4622)
                     /locus_tag="DHV81_08285"
     CDS             complement(4392..>4622)
                     /locus_tag="DHV81_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94440.1"
                     /translation="DLLTGILRDEWGYEGMVTTDWWTCGEHYKETKAGNDLKMGNGYP
                     DRVKKAYDKGAISRSEMETSVKRILGLILKLD"
BASE COUNT         1450 a          756 c         1041 g         1375 t
ORIGIN      
        1 ataaagacag gtgcaccttc gcgctcagag cgtgttgcaa agtataacca gcttttaaga
       61 attgaagagg aactgggaaa gagcgcagtt tatccgggaa ttaaagcgtt taatgtgcag
      121 cagtagttta aaataaaact ctatgactgt attcagtcat tatccattgg agtgtcttta
      181 ggagaaaata agataagtaa agtcgcagat atcataatat ggtatctgcg atttttgttt
      241 ttatcagtaa tccttaactt tttcaaacaa cttttttacg cgtctcaggt tactatttcc
      301 tcatttttaa tgtgacattt acccattaat tgtagccctt aattgtagca attaaaaggt
      361 atttatcgta acacgataaa aaaatattgc aaaactaaaa gcagtgtagt acaattagct
      421 tgccggtaag tgaaaaatat gagaaaataa ctacatagat agtaaggagg tagctatgga
      481 caataataat atcgggggca tgaatcccca acagttttca cagaacacgc cacagacttc
      541 acagccacat atgggaatgt caggtatcga actgcagaat atgcagcagg aggcggagca
      601 aagacggcgt gagcaaagca ggagaaacgc ggatttcttt ggcaggctat gtatacctac
      661 gataatatat gcgctgctgt acaccatttt cctgtacgag aatacaggtg gaatacttgt
      721 tacgttgttt gcaatcgtca caggagtata cagcctgtat tgtatgaaga tacttcatat
      781 agaggcaaag ccactcactg tatggtacag tgtgatgatg atactcacag gcatatcatc
      841 aggcttgacc ggtaataaaa ttatacaggg atttaatttt tgctggatac ttgtgtttct
      901 tgtttttatg ctgcttcaca atttctgcaa tgacagacag tgggggctta taaagtatat
      961 cgctgcggca tttcaggcag tgtttggagc gattgggtgt atagctgagc cgtttatgga
     1021 tattgccgac tatatgagaa atgaacgcat ggatagtgac aatatgggca gtgacagtat
     1081 ggtcggtgac agtgcaaatg caactgcagg agagcggcat gtgaaaaaac acagaatgct
     1141 ttatgtgttt atcggaatag caattgcctt tcctctggtt gtgctgatag tggtgctgct
     1201 gtgctctgcg gatgcggttt ttgcctcagt aataaagaaa atatttgctg atatcaattt
     1261 atttacagtg agcaaggttg tatttctttt tgtatttgcg cttttctcat cgtactgtgg
     1321 tataaagtat ctgagcaaga agcgtatatc ggatgcaccg gttgagactc cggcatttcc
     1381 ggctgcaatc ggtatcactg tagctgcaac tatatcagtg gtatatgtat ttttctgttt
     1441 tatacagatt gtgtatctgt tcgggggact tatgcagctt ccaagcggct atacctacgc
     1501 caggtatgcg agagaaggct ttttccagct gcttttcgtg tgtatactta atgtgattat
     1561 cgtattgctc ggaagtgaat tgtttagaaa gaataaaata ttaaatgcat ttttaatttt
     1621 aataacattg tgcacatata tcatgattgc atcaagcgca taccgtatgg gactgtatgt
     1681 gtcagagtac gggctgacag ctaccaggct ttgtgtgttc tgggcacttg gagtgatagc
     1741 gctgtttatg ctcggagtga ttttatctat atgtaagcct gctttttcgc tatttcggta
     1801 cggaataata gtaatcgggg tatgttatct tgtgcttgca tttgcaaggc ctgattatct
     1861 ggttgcaagg tacaatacag tatgtatgga ggatacagac tacaaatatc tgatgtcact
     1921 ttccacggat gcatctccgg cgcttgcagc agatgcagac tttatggaaa ataagggtat
     1981 ggtcaccatg tatgcaagac agctggcagg tgagacaaat gacagcttaa gacagctcaa
     2041 tgtatctcat ataaaggctg cacatttatt cagagatagc atagatgagg taaagagctc
     2101 acagcttatc cttttatatg tatattcgcc ttacgattcg ggcagctata acaacaatga
     2161 taccggtctt gatggcgtgg acagcataca gatgggctat catgtgttaa aggatacaga
     2221 agatgatgac acagccaact atgattacga cagttatagc atggatgata caagagtggc
     2281 ggcaccagta tttttcaaat gggtggatgc agtcgaggtg aagaagattt ccgacagtga
     2341 gcgtatcttc cttgcaaaga ttccaagaaa ggcattaaaa ggcaaagatg gagtgaatat
     2401 tgaatacaga tttaataaaa atggagatgt tatatattca tcccagtaca atgtgattct
     2461 tgacaagaaa aaaggactca acgaggtgga gctgtcatac tatgccggaa cagatggcgt
     2521 ggatgtgcct gagtacaata tttatggaaa gtaatagttt aaattgtata taattcaaag
     2581 ttgatatggt gaagttgatt aaaaactgct atacttaaca tgtagtatag gttgttaaat
     2641 tatttcatta aggtaggaaa attcatggga agatttttaa atccgggaaa taaagctttt
     2701 caaaagacat tgaaatcgga aatatatgtg gataaaacta tgcttttggc atacaccaat
     2761 aaggttatag gttctgatat ggcatgcata tgcaatagca gaccgaggcg ctttggaaag
     2821 tccatcacgg cgaatatgct tgctgcttat tatagcagag gatgtgattc ctttgatatg
     2881 ttttccactc taaaggtagg caggcttgat tcctttgaga caaaccttaa taaatatgat
     2941 gttattcata ttgatgtcca gtggtgcatg atggatgcgg gcgaagtgca aaacaccgtg
     3001 aaatacatca ataacggtat tcttgatgag cttataatag catatggtga tgtgataccg
     3061 gacactgtta aaacagccta tggagccatg tcttatataa acgcagccac aggaaatacc
     3121 tttgttatca taatagatga gtgggatgtg ttgataagag atgaggctaa taataaggaa
     3181 ctgcaggagg aatatattaa tttcctcaga ggaatgttta aagggacaga gccgacaaag
     3241 tatattgcgt tagcttatct taccggcata cttcctatta aaaagttaaa aactcagtca
     3301 gcacttaaca attttgaaga gttcacaatg cttgatgcag gagcgcttgc atcatatatt
     3361 ggctttacag atgatgaggt taagcagtta tgcaaaaagt atgacaggga ttatgaagca
     3421 gtaaaaaact ggtatgacgg ctatatgctt tcgggtaaac atgtgtataa tccaaaagcg
     3481 gtagtgagcg tgatgatgag aggcagcttc caaagctatt ggtcacagac cggcacatat
     3541 gaatctctga ttccgcttat tgacatggat tttgatggat taagagccgc tattatttca
     3601 atgatttcag gaaatgagat aaaagttaga acaaccacgt ttcagaatga tatggtatca
     3661 tttaaaaaca aagatgatgt gctgacattg cttatacatc ttggatatct ggcttttaat
     3721 cagaaaaatc agatggcata tattccgaat gaagagcttc gcaatgagct tatggatgcg
     3781 gttgaggaaa acaaatggga tgaaatcata cagtttgaga gacagtcaat agatttgttt
     3841 aatgcaacaa taaacaaaga tgtaaatact gtggcagcaa aaattgagca gattcatatg
     3901 gagtatacct cggtgattca atataatgac gagaactccc ttagcagtgt gttggccatt
     3961 gcttatcttg gaacaatgaa ctattatttt aagccggtca gggaattacc gacagggcgt
     4021 ggctttgctg attttgttta tattccgaag cctgagtata taaatgatta tccggcgctt
     4081 gttgtggagc tgaaatggaa taagaatgcg gatacagcca tgcagcagat tcgtgagaga
     4141 cagtatccga attcattatt gcaatatacg ggaaacatcc tgcttgtggc aatcaactat
     4201 gatgaaaaaa caaaagagca tgtatgtaaa attgaggaat acgctaaaat acagaattaa
     4261 tgattgagat atacaaatat aaaaacaaat acaaaaacgg acatggcagt tttgcatgtc
     4321 cgttttttgt ttttgtataa taagtatcgg gtattatgaa ccgggtaggt ttacaaaaag
     4381 cagagtaaaa tttaatcaag ctttaatatt aatcctaata tacgctttac agaggtttcc
     4441 atctcactac gagagatggc acccttatca taagccttct ttacacgatc aggatatcca
     4501 tttcccatct taagatcatt tccggctttt gtctccttat aatgctcgcc acaggtccac
     4561 cagtcggttg tgaccattcc ttcatagccc cactcatcac gcagtattcc tgtcaaaagg
     4621 tc
//
LOCUS       DPUF01000240            3725 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13780, whole
            genome shotgun sequence.
ACCESSION   DPUF01000240 DPUF01000000
VERSION     DPUF01000240.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13780"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="DHV81_08290"
     CDS             <1..660
                     /locus_tag="DHV81_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthetase family
                     protein"
                     /protein_id="HCI94441.1"
                     /translation="LLINLVMYTLSLLTIGLLCMIFKYPLLKNFSGISRIFICLGIVV
                     LIFLALVFYMLLRKGSILYSICDSLIRLFEKIHIIRHGDRLRIKLKNTMKEYQECSNS
                     ITGQTGLLIEIFFWNIMQRVSQLLVSFMIFMAVGEGINKAVDVSVIQCFVAMGSNCVP
                     IPGAMGVADYIMIDGFKQLVGSNAANMELLCRGMTFYGSVITSAVIILIGYIIRKKVA
                     K"
     gene            758..1798
                     /locus_tag="DHV81_08295"
     CDS             758..1798
                     /locus_tag="DHV81_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCI94442.1"
                     /translation="MSSNDSTAKEQSFLFNVNKIFLVIHLLMLFMFSSLGVDLMAGVS
                     LISICFYFLAFSLTKSEKLSIYVYSVAVEILLYMILAVVCLGIQCNFQLFLIDAMFFL
                     FSMDYVVLRKKKKNHVAILLCCVYAIALIILYMLDGFYAPLYKLDSVVIKSISIAMIS
                     GVVFLIITCMMCLLHFMSSEEGAMEKQAQFDALTELPNRFYMMAKLKNLFEAEKQGEY
                     FLAMIDIDDFKKINDSFGHNVGDDALKMLARTIVNKARGVELSYCRWGGEEFLLLGKC
                     VDGEVPKNFLDELRVEINSKSFWTSKGSGRMTVTIGAGKYKVGQDYKEWINFVDKQLY
                     VGKCTGKNKVVC"
     gene            1960..2045
                     /locus_tag="DHV81_08300"
     tRNA            1960..2045
                     /locus_tag="DHV81_08300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:1993..1995,aa:Leu,seq:caa)
     gene            2240..3725
                     /locus_tag="DHV81_08305"
                     /pseudo
     CDS             2240..3725
                     /locus_tag="DHV81_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568079.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     assembly_gap    2407..2472
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1050 a          563 c          821 g         1225 t
ORIGIN      
        1 ctgctgatta atcttgttat gtatacactt tcacttttaa caattggctt actatgcatg
       61 atttttaagt atccattgct gaaaaatttc tcaggtattt cgcggatatt tatatgtttg
      121 gggattgtgg tgctgatttt tctggctctt gtattttata tgctgctaag gaagggaagc
      181 atactatact caatttgtga tagtcttatc cggctttttg aaaaaataca tataatacgg
      241 catggtgaca ggcttcgaat aaagcttaaa aatactatga aagaatatca ggagtgttca
      301 aacagtatca cagggcagac agggcttctc atcgagattt tcttttggaa tataatgcag
      361 agagtttcac agcttttggt atcgtttatg atttttatgg cagtcggcga aggcataaat
      421 aaggcagttg atgtgagtgt gatacagtgt tttgtggcga tgggctccaa ttgtgtgccg
      481 atacccggtg ctatgggcgt ggctgattat attatgattg atgggtttaa acagcttgtt
      541 ggtagtaatg cagcgaatat ggagcttttg tgcaggggaa tgacatttta tggaagtgtc
      601 ataacaagtg ctgttattat tcttatcgga tatataatta gaaaaaaagt agcaaaatag
      661 tctagtactt ttgcacaata atatattgtt atatttgtta aaatgtgtta ttattctata
      721 gtaatatatc agatattttt tatgaaggta ttggacaatg agtagcaatg atagtactgc
      781 taaggagcag agctttctat ttaatgtaaa caaaatattt cttgtcatcc atctgctgat
      841 gctatttatg ttttcatcat taggtgtaga tttgatggca ggagtcagtc tgatcagcat
      901 atgcttttat ttccttgcat tcagtctgac aaaatctgaa aaactgagca tatatgttta
      961 tagtgtggct gttgagatat tgctttatat gatacttgcg gtggtttgtc tcggaattca
     1021 atgtaatttt cagctttttt tgatcgatgc aatgtttttt cttttttcca tggattatgt
     1081 ggtcctcagg aagaaaaaaa agaatcatgt tgccattctg ctttgctgtg tatatgctat
     1141 tgctctgatt attctttata tgcttgatgg cttttacgca cctttatata agttggattc
     1201 tgttgtaatt aagagtattt ctattgctat gatttctggt gtagttttct tgataataac
     1261 atgtatgatg tgccttttgc attttatgtc ttccgaggag ggggcaatgg aaaagcaggc
     1321 acagtttgat gcgctgacgg agcttccaaa tcgtttctat atgatggcga agcttaagaa
     1381 tctttttgag gctgagaagc agggggaata tttccttgca atgattgata ttgatgattt
     1441 taagaaaatt aacgattcat tcggacataa tgtaggagat gatgcactta agatgctcgc
     1501 tcgtactatc gtaaacaagg ctcgaggtgt tgagcttagc tattgcagat ggggaggaga
     1561 ggagttttta cttcttggca aatgtgtcga cggagaggtg cctaagaatt ttctcgatga
     1621 gcttcgtgtg gagattaatt caaaatcctt ctggacatct aagggcagcg gaaggatgac
     1681 agtgaccata ggtgcaggga aatataaagt ggggcaggat tacaaggaat ggattaattt
     1741 tgttgacaaa cagctttatg tcggaaaatg caccggtaag aataaggttg tatgctgaga
     1801 catatttcaa ggctttgaca tttttaaatg agctgtaatc gttgctggtc acactgttag
     1861 gcgtgaacag tcacaatcgc attgaaattt atattgaaat tacaaatttt agttgcatga
     1921 agtcaataac tgtgatagaa tattgtctgt tcgtaagacg ccggcatgtc ggaatggcag
     1981 acgatgcaga ctcaaaatct gttgggcgca agcccgtgtg ggttcaagtc ccactgccgg
     2041 cactatttgc aaaaccccgt aaatccaaga ttttcagtaa atcaagggtt tgcggggttt
     2101 ttctttactt acaatctttc aaaaaattaa agaaattagg gtacttttaa gaaaaatttg
     2161 ccccaaaatt gtactaaaaa aggtacccaa aaatctgcca tataggatgt aataccagat
     2221 agcaaacagg agcattatta tgataaaaaa gatttataaa aaatatgaaa ttatattcaa
     2281 tgttgtatta ttctcgattt ttccgattgc ggctttttat cttatggagt tctacgaaca
     2341 taatcctttt gaagaagtca gatttatggc gggatttttt aatattattt tgtttgagct
     2401 gattgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nngtggccat ggtttttgga ctgattaatc attatgttat gcttttccgc
     2521 tcgactcctt ttgttccatg ggatattttt tcaattggta cggcgacaag tgttgcatcc
     2581 aattatgatt tcgcaccgac tgcaggagta gttgttgtta cagttatatt tatagctctt
     2641 attatgctga tgcattttgt tgatttcaga attaagtgga aattcaggtt cagactaatt
     2701 ccgacagtgc ttggattact tgtgatgtgc ctgtttgtga atgccctgca ggatgaggat
     2761 ttccagacgg acaattacct gtatccgttt ctatttacac ctgcatatat gacaaaggtg
     2821 aatggaatgg cggtgacctt tgcgatggat cttaagtttg tggcggttga taagcctgac
     2881 gggtatagca gacagaaggc taaggcgctg cttgacagct atgccggtac agatgataat
     2941 tcagatgcag ctaataatac ttcaattaca gataaaagtg attatccgaa tattatagtt
     3001 gtgatggatg aggcattttc agacctgtca gtgctcggtg attttgatac aaatacagac
     3061 tatatgccgt ttgtgcacag tcttgaaaag ggcaatgaaa acaccataac aggatatctg
     3121 aatacgtctg tatgcggtgg aaataccgcc gatactgagt ttgagtttct gacaggcaat
     3181 acgatggctt ttcttccggt aggaagtatt ccttatcagc agtatataaa gagcacgaca
     3241 ccttcccttg caagctattt aaaaagcata ggctacgcaa cgtatgcaca gcatccgtac
     3301 tatggcagtg gatggaaccg ggatacggtc tatcctcttt tgggatttga caatctttcg
     3361 tttatgcagg attattctaa tcagaggttt gtgcgcaaat atataagtga tgagacatca
     3421 tttgacagga ttattgagac ctatgagaac aaacaggatg gtcagccggc atttatattc
     3481 aatgtcacta tgcaaaacca tggtggatac acggatacct actacggctt tgacaatacg
     3541 gtgacagcgg ataagctcaa taattctgca cttgaccagt atctttcttt gattaagatg
     3601 acagacgagg atttaaagaa cctcattgaa tatttctcaa atgtggatga gaaaactata
     3661 gtggtatttt tcggtgatca tcagccaaat gatactgtgg caagctctgt tcttgcagca
     3721 aacgg
//
LOCUS       DPUF01000241            6356 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12125, whole
            genome shotgun sequence.
ACCESSION   DPUF01000241 DPUF01000000
VERSION     DPUF01000241.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12125"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(1..845)
                     /locus_tag="DHV81_08310"
                     /pseudo
     CDS             complement(1..845)
                     /locus_tag="DHV81_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516538.1"
                     /note="too many ambiguous residues; incomplete; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     assembly_gap    516..630
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(946..2136)
                     /locus_tag="DHV81_08315"
     CDS             complement(946..2136)
                     /locus_tag="DHV81_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCI94443.1"
                     /translation="MHGRKKIIIYILISIIGLFWLSGCDSPSSDSGNTESKAEAEAQN
                     GLIYKSSMKLLYAKNFSVDYYEGGYKILTTKDGTKILTVPKGRKTPKDIDKDIIVLKE
                     PVSDLYLVASGVMDMFDKLDAVDTIKFSGLDSDGWYIDSARKALESGRMLYAGKYSKP
                     DYELLVSENCSLAIENTMITHSPQVTEKLKSFDIPSIIEYSSYEEEPLGRVEWVKFFG
                     ALTDRDEKADELFNEQVDIVNRIAKTDGTDTAANADSRPTVAFFYITSNGQVQVRKST
                     DYVPKMISLAGGKYIFDASNDDDTGRSTMNMQLEDFYNGAIDADYLIYNSAIDGGVKN
                     LNELLDKCPVLADFRAVKGGNVFCTTNDMYQQSMSIGYMIDDMHSMITGAPDSGMKYL
                     FKLK"
     gene            complement(2144..3055)
                     /locus_tag="DHV81_08320"
     CDS             complement(2144..3055)
                     /locus_tag="DHV81_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCI94444.1"
                     /translation="MRKLFRNKFTYIIALALVCAMSLAACGNKNAKAETESKKAVQDA
                     TESTKKAEKTEKIALSDGTYTAEFTTDSSMFHVNDAYDNKGTLTVKDGKATIHVTLTS
                     THIVNLFLGTAKDASKDKAELLEPTVETVDYGDGTTEEVNAFDIPVPVLDEEFDLALI
                     GTKGKWYDHKVKVSNATPADADASVSESAGKELPDGTYNINLDFEGGSGKASIASPAE
                     VTISGGAATATVQWSSPNYDYMIVGGQKYMPVNTDGNSVFEIPVKLDTPMQVIGDTVA
                     MSKPHEVEYTLTFHSDSATEVLGEDGE"
     gene            complement(3113..>3489)
                     /locus_tag="DHV81_08325"
     CDS             complement(3113..>3489)
                     /locus_tag="DHV81_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt chelatase"
                     /protein_id="HCI94445.1"
                     /translation="VFMGHGTSHTAKISYSQMQSQMTDLGYENVFIGTVEGEPEDTSC
                     ESVIEKVSAAGYKNVILRPLMVVAGDHANNDMAGSDDDSWLSQFKASGKFDKIDTQIN
                     GLGEIEGIQKLYVEHTAAAMSK"
     assembly_gap    3490..3613
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3614..4364)
                     /locus_tag="DHV81_08330"
                     /pseudo
     CDS             complement(3614..4364)
                     /locus_tag="DHV81_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516541.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt chelatase"
     assembly_gap    3832..3909
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4365..4481
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      complement(4602..4784)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4920..>6356)
                     /gene="cls"
                     /locus_tag="DHV81_08335"
     CDS             complement(4920..>6356)
                     /gene="cls"
                     /locus_tag="DHV81_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCI94446.1"
                     /translation="SVASRVFQDLLYVAAILLSLYIVNRRMKMYIKMSWIFLILSVPI
                     VGIPCYFFFGRPELTSKTQKRMARIVEAYAPLRPENELLNETLRLTDMDAYRQSRLIT
                     QNQKYSMYAEDCTEYFKSGEEAFESILKDLRSAEKFIFMEYFIIGSGVMLDQILYILK
                     EKVKHGVVVRIIYDDFGSINAIPPHFIKEMESIGIQTRRFNPYKPMLSVIMNDRDHRK
                     ILVIDGRVAHTGGYNIADEYINKKIRFGYWKDAGIRVEGECVNSFTTMFLEMWNYINK
                     DNAVHDEYLNPHNTFSQSEKSGFVQPFCDSPLEHDDVGENLYVSIISRAKKYVYIFTP
                     YLILGTELSHAMISAARSGVDVRIVVPKIPDKKLIYLQTKANFRPLIEAGVRIYLYTP
                     GFIHSKCIVADDDTAIVGTCNLDFRSMYWNFEDFVYMYRTQCIGKIKEDAIETFAVSE
                     EVYEESTNDISFAGRLLQSILQLFAPLL"
BASE COUNT         1554 a         1523 c         1022 g         1823 t
ORIGIN      
        1 agcatgcttt ttactatgtg tggtgcagct attccgacaa acgatatcgg tcccgcaaac
       61 gccactatac atgcggaaag caggcttgaa agcaatacca gaagcacccg gatgcgcttt
      121 atgtttacac ccagatttac ggcatatgac tctccgagct gatatgccat aaggggcttt
      181 gacatgagaa aaacaaaaat aaatgtgatt cccaccacaa tgctcatcac cccgacgctg
      241 tcccaggtca tgcccgaaaa gcttcccctt gaccagttat gcaggtttac tatgtcggaa
      301 tcatctgcaa atgtcaccac aaactcagtc accgcagagc atatgtagcc aatcatgaca
      361 ccgctcacca caagcattga catgctgctt acctttcttg aaatgataag cacaaagccc
      421 attgaaagca ttgctcccaa aaacgctgct gcaatcatgg ccagtgagct cacacgtatg
      481 gcatttccaa gtaaaaatac cataacaagt gccacnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccccgccca gtatcatcgc tgcgatagct
      661 cttggcatgc ggatatccca cacaatacgt cctaccctgt ctgagtggtc ataaagtgtc
      721 gcaaatgcag ccttaaacga tatgtgtaca gttcctatgc acacattcat gataagcaat
      781 ataataatta agattataag aataataaat gttgtaaaac accttatttt tctgtttttt
      841 tccatgttgt agctccgctt tttatctttt tgtgctattg attttcctgc attctgtcat
      901 atccggtaac aattcagccc accacataca ttccacaaat cacagttatt tgagcttaaa
      961 cagatatttc atgccgctgt cgggtgcacc tgtaatcatt gaatgcatat catcaatcat
     1021 atatccgatg gacatagact gctgatacat atcgttcgtg gtgcaaaata cattgccacc
     1081 tttgacagcc ctaaaatcag caagcaccgg gcacttgtcc aaaagctcat tcagattctt
     1141 cacaccgccg tctatcgcac tgttatatat cagatagtca gcatctattg ctccattgta
     1201 gaaatcctct agctgcatat tcatggttga cctgcctgta tcgtcgtcat ttgacgcatc
     1261 aaaaatatat tttccgccgg caagcgaaat catctttggc acataatctg tgcttttgcg
     1321 cacctgaacc tgtccattgg atgtgatata aaaaaatgca actgtcggtc tgctgtcagc
     1381 atttgcagca gtatcggtac catctgtctt tgcgatgcga tttacaatat ccacctgctc
     1441 attaaagagc tcatcggcct tttcatcccg gtctgtgagt gctccaaaaa acttcaccca
     1501 ctccactctg ccgagcggct cctcctcata gcttgagtac tcgattattg aagggatatc
     1561 aaagctttta agcttttccg ttacctgcgg cgaatgagta atcatagtat tttctatggc
     1621 aagagaacaa ttttctgaca ccaaaagctc ataatcgggc tttgaatatt tgccggcata
     1681 aagcattctg ccactttcaa gagcctttct tgcactgtca atgtaccagc catcactgtc
     1741 aagccccgaa aatttaatag tgtccactgc atcaagcttg tcaaacatat ccatcacacc
     1801 tgatgcgacc agatacaaat cacttacagg ctccttcaat acaatgatat ccttgtcaat
     1861 atcctttgga gtctttctgc cctttggcac ggtaagtatt ttggtaccgt cctttgtcgt
     1921 aagtatcttg tatccaccct cataatagtc aacagaaaaa ttctttgcat ataaaagctt
     1981 catactgctc ttatatataa gaccgttttg tgcctctgcc tcagccttcg attcggtatt
     2041 ccctgaatcg gaagaaggac tgtcgcagcc tgacagccag aacagtccta taattgatat
     2101 caatatatat ataattatct tttttcttcc atgcatagct taattattct ccatcctcac
     2161 caagcacctc tgtagcagaa tctgaatgga aggtaagtgt gtactcaacc tcgtgcggct
     2221 tgctcatagc aactgtatct ccgataacct gcataggtgt atcaagctta accggaattt
     2281 caaatacaga attgccgtct gtatttacag gcatatattt ctgtccgcca actatcatat
     2341 aatcatagtt tggactgctc cactgcactg ttgcagtagc ggctccaccg cttatagtca
     2401 cctcagccgg tgatgcaatg ctcgccttac cgcttccgcc ctcgaaatca agattgatat
     2461 tgtatgttcc gtcaggaagc tcttttcctg cactctcaga tacgcttgca tctgcgtccg
     2521 caggtgtggc atttgaaacc tttaccttgt ggtcatacca tttgcccttt gtaccgataa
     2581 gtgcaaggtc aaattcctca tccaaaacag gaaccggtat atcaaacgca ttgacctcct
     2641 cagttgtgcc atcgccataa tcgacagtct ctacagttgg ctctaaaagc tcagccttgt
     2701 cctttgaggc atcctttgct gttcccaaaa agagattgac aatgtgtgtg gatgtgagag
     2761 taacatgtat tgttgccttg ccatccttca cggtgagtgt acctttgttg tcatatgcat
     2821 cattcacatg aaacattgag ctgtctgtag taaactctgc tgtatatgta ccatctgaaa
     2881 gtgctatctt ttctgttttt tcagcctttt ttgtgctctc tgtcgcatcc tgcactgctt
     2941 ttttgctttc tgtttcagct tttgcattct tattaccgca ggctgccagg ctcatggcac
     3001 aaaccagtgc cagtgcaatg atgtaagtaa acttgtttct gaataatttt ctcatgtctg
     3061 atatactgct cctgttattt tatagcttcc ccactataca tttaagtaac tgctacttgc
     3121 tcatagcagc cgctgtatgc tcaacataaa gcttctggat accctcaatc tcaccaagtc
     3181 cgttaatctg tgtatcgatt ttatcaaact ttcctgatgc cttaaactgt gaaagccatg
     3241 aatcatcatc acttcccgcc atatcattgt tggcatggtc tcctgcaaca accataagcg
     3301 gacgaagaat gacattcttg taaccagcgg ctgacacctt ctcaatgaca gactcacatg
     3361 atgtatcctc cggctcaccc tctacagttc cgataaatac gttctcataa cccaaatctg
     3421 tcatctggct ctgcatctgg ctgtatgaaa tctttgctgt atggcttgtt ccatgtccca
     3481 taaatacaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnngcatcac ttccaacctc accgaggagt ggctctgcaa tctttacaga
     3661 ctcaaattta tccttatact tctctacagc ctctgaaagc tcatcatact ctgcgccatg
     3721 catcaggtgt gtaggctgta ccacaagatt ctttacacca tttttgactg cacgctcaag
     3781 tgcctgatcc atattgtcaa tcttctcgtc atctcttgcc tgtacatgat tnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnng ctgctattgc atcctccaca cccttgatat cagccacacg gctgtcgttg
     3961 aaggatgtac caaagcttac tacaagaatc tcattctctc cgatatcatc ctggtttctc
     4021 ggatcgtcct ttgaggcatc acctgtatct ctgccgaaat agtcaggatc agcattttct
     4081 ccctctacaa gctccttctg tgcatctgtg agcttgtccc aggcctcctt agcctccttg
     4141 cactgcttat ctgtatcatc tgttctctcc tgcacgtaga ttgcatcgat taaatctgcc
     4201 acatgcattg ctgcctcctg atctgctttt gcgcttgtct cactctctga agccttagat
     4261 acctgtgtat cattctttgc atcatctgcc ttgctgccac agccggtcac tgctgtcata
     4321 gccataactg ctgccatcag tactactact aatttctttt tcatnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcaaagag ctatacatgt
     4501 caaaatcctt ctaacccaga ctatacggct atcataacct tgtctgtaat tataattatt
     4561 tcatcacgta cagccagtta ctcgtggctt gaaacactat tgtttccgta caacacaaag
     4621 gcaggtctcc cggcttaaat atcattgctt cggccgtctt cccggttgcc cagtgaccta
     4681 tcggccgtca ctccttaatt acggtgacga gttcgtacag gatttgcacc tgtttccctt
     4741 ttcaccggag ccagcttcac atcgcctgtc ataaatcaga ttcaaatgct tgtctgctcc
     4801 gacaccttta tgctacatat tcagtttctt cgtgcgttgc ttacgcaatc gcttggatta
     4861 ttatattaca acttgtaaca ttattcaaca gatttttatc agacgcttta cagtcacatt
     4921 tacaaaagcg gtgcaaaaag ctgcaaaata ctctgcagca gcctgcctgc aaatgagata
     4981 tcatttgtgc tttcctcata cacctcctct gaaaccgcaa acgtctctat cgcatcctct
     5041 tttatttttc cgatgcactg agtcctgtac atgtatacaa aatcctcaaa attccagtac
     5101 atgctcctga aatccagatt gcatgtgcca actatcgcag tatcatcatc tgccacaata
     5161 cactttgaat gtataaagcc gggtgtgtat agatatatcc ttacccccgc ctcgataaga
     5221 ggtctgaaat tggcttttgt ctgcagataa atgagctttt tatcaggaat cttaggtaca
     5281 acaatacgca catcaacacc tgacctggct gccgatatca tagcatgtga cagctctgtt
     5341 ccaagtatca gatacggtgt aaaaatataa acatattttt tagctcttga gatgatactc
     5401 acataaagat tctctcctac atcatcatgc tcaagtggag aatcgcaaaa cggctggaca
     5461 aaaccgcttt tctctgactg gctgaaggta ttgtgtggat tcagatattc atcatgcaca
     5521 gcattgtcct tattgatata attccacatt tcaaggaaca tcgtcgtgaa actgtttaca
     5581 cactctccct ccacacggat accggcatcc ttccagtagc caaacctgat ttttttgttg
     5641 atatactcat ctgctatatt gtaaccaccg gtatgcgcca cacgtccgtc tatcaccagt
     5701 atcttcctat gatccctgtc attcataatt actgaaagca tgggcttata tggattgaag
     5761 cgtctggtct gtattccgat gctctccatc tcctttataa aatgtggcgg aatggcattt
     5821 atgctgccaa aatcatcata tatgattctc accacaactc catgctttac cttttctttg
     5881 agtatataaa gtatctgatc aagcataacg ccactgccga ttatgaaata ctccataaag
     5941 ataaactttt cagctgacct taaatctttt aaaatgctct caaaagcttc ctcacctgat
     6001 ttaaagtact ctgtgcagtc ctccgcatac attgaatatt tctgattttg agtgataagc
     6061 ctgctttgtc tgtatgcatc catgtctgtc agtctgagtg tctcatttag cagctcgttt
     6121 tcaggccgaa gcggcgcata tgcttccaca attcttgcca ttcttttctg tgtcttcgac
     6181 gtaagctccg gtcttccgaa aaagaaatag cacggaatac caacgattgg aacactcagt
     6241 atcaaaaata tccatgacat ttttatatac attttcatcc gcctgttgac aatataaagc
     6301 gaaagcaata tggctgccac atacagcaga tcctgaaaca ccctgctggc aacaga
//
LOCUS       DPUF01000242            3753 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8289, whole
            genome shotgun sequence.
ACCESSION   DPUF01000242 DPUF01000000
VERSION     DPUF01000242.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8289"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(1..483)
                     /locus_tag="DHV81_08340"
                     /pseudo
     CDS             complement(1..483)
                     /locus_tag="DHV81_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743125.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
     assembly_gap    162..220
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(659..1012)
                     /locus_tag="DHV81_08345"
     CDS             complement(659..1012)
                     /locus_tag="DHV81_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCI94447.1"
                     /translation="MNKRSVGSIYEQLAAEQLINMGYRVLIRNYRNRYGEIDIIAKDG
                     DTICFCEVKYRRDNGCGSALEAVGYSKQKRIISVARYYLMTHGLSEWTPCRFDVIAVD
                     DDEVTVLKNAFEGSQ"
     gene            complement(1042..1353)
                     /locus_tag="DHV81_08350"
     CDS             complement(1042..1353)
                     /locus_tag="DHV81_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HCI94448.1"
                     /translation="MHGEKNNRNNNGIDTGKTAVAIEYVPGEAAPKILATGKGIVADK
                     IIERAKESNVPTYKDDKLADTLSKLKIGDMIPPELYEVVAEILVFVNDLDKLKAKIDQ
                     R"
     assembly_gap    1419..1544
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1545..3196)
                     /locus_tag="DHV81_08355"
     CDS             complement(<1545..3196)
                     /locus_tag="DHV81_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HCI94449.1"
                     /translation="MQISNLVSQYNNSVANGEPMTGAKGVEKLVSSLNEMSKGMIFEG
                     TVSSVRGNQVKLALSNGQQILARLAGKFSFEQGQSVFFQVKNNDGGTIEIKPYTVDGE
                     GVNLTLMDALKAAGLPVDGTNLSMVNKMMEEQMPIDKISLNQMYQLVQDNKGINVTTL
                     VELKRLGIEINQVNAAQFENYANDKQAITIAMDSLIEELPNALSAEDLSMYKLVSQAR
                     DILNIVTDGLPEDAFISNEDADMSQYEAIMRDNQSAPVVKKHFNIAELFESLNSVSGE
                     SQDIQTTQKINNSSVIDNIFLQGNEAKSNTIGFILSDKQIEELNEQVRKLLPDLQENN
                     ISLYSEDSSVVGILNDINSMLENTPANADALRQLFSGEAFKLMLKETLEQQWMIKPGD
                     LEKNPKKIDGLYDKIEKQIINMENILKTSGVVNPKAEALADNIRGNIEFMNQINEAYT
                     YMQVPLRMNEGNASGQLYVYTNKKSISDPDKELSAFLHLDLEYLGGTDVSIKMLHRKV
                     TTNFYLDSDESYAMVKQFLPVLEKRLQDKGYNCELTVNSDSK"
     gene            complement(3358..>3753)
                     /locus_tag="DHV81_08360"
     CDS             complement(3358..>3753)
                     /locus_tag="DHV81_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94450.1"
                     /translation="LSRKVNKEADKIRGFIRFSDNGNYLLAQFEPKCDMIPIVMWHFV
                     DRYPGENFVIYDERRRYAVVHPRLKECFYINESDFKQLEKSVPGKTDDFEQLWKVYFE
                     YTDIKERFNPQCQRNLCPKWYRKNMVEFT"
BASE COUNT          992 a          851 c          472 g         1253 t
ORIGIN      
        1 tcattcgtga taagtccgtc cacatctgta tatggtctct ttcttgtcac cccgatgcca
       61 cactgctttt ttccaactct caaaacattg gtagtatgtg tctggtctgt cgtaacaatc
      121 tgccctgcct ccgctccgat tgcctccgcc acccttttat annnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccacgtgtaa agcttagatt
      241 taagctttca aacatccctt cactgagtcc accaagacgt gttgtaaagc aatgctctat
      301 catagaaagc tcatttagct tcttaaaatg taacaaagga acaacacttc cgcttgaaag
      361 gacgtgcttt tttaattcca gaacatcttt atcattcttt gccacaaaat catttatagt
      421 aatttcatca acacctttgt gtatgttttc agcctgtata ttcttatcaa tctctgtctt
      481 catatttata tctatgcaat tatatatttc cgataactat tgctgcttat aattactatt
      541 tgtagttatg ttacttctta taattgcctg ctccttatct gtttttatga aatagtctgt
      601 ccaaacgttt ataaattaac ggtccagtaa tacctgtcca tcactgccga gataatagtt
      661 attgactgcc ctcaaatgca ttcttcaaca ctgtaacctc gtcatcatcc actgctatca
      721 catcgaatct gcatggtgtc cattcactca gaccatgtgt cataagataa tagcgagcaa
      781 ctgatatgat tcttttctgt ttgctatacc ccactgcttc aagagcgctt ccacaaccat
      841 tatccctacg atatttcacc tcgcaaaaac atattgtatc tccatccttt gctattatat
      901 ctatctctcc atagcgattt ctgtaattgc gaataagtac cctgtatccc atatttatta
      961 actgctcagc tgcaagctgc tcgtatatac ttccaacact tcttttattc ataactgcct
     1021 caaatactgt aaaccttcac attaccgttg atcaattttt gccttaagct tatccaaatc
     1081 attaacaaaa acaagtatct ccgcaacaac ctcatacaac tccggtggaa tcatatcacc
     1141 tattttaagc ttagacagag tatcagcaag cttatcatcc ttatatgtag gcacattgct
     1201 ttcctttgcc ctctcaataa tcttgtcagc tacaatgccc ttgcctgtag ctagtatttt
     1261 gggtgctgct tctcccggca catattctat agctacggct gttttgcctg tgtctattcc
     1321 attattgttg cgattattct tttcgccatg catacaactc acctgtcttt ctaaagtaag
     1381 ctttaattat gtaactattc ataaattatc ttatacacnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgtttc gaatcactat
     1561 ttacagtcaa ttcacaatta tagcccttat cctgcaatct cttttccaac actggcagaa
     1621 attgctttac cattgcatat gactcatcac tatccaaata gaaattagtt gtcaccttcc
     1681 tatgaagcat tttaattgac acatctgtcc caccaagata ctctaaatcc aaatgtaaaa
     1741 atgctgataa ttctttgtca gggtccgata tacttttctt atttgtgtaa acatacaact
     1801 gtccgctggc atttccttcg ttcatcctca atggaacctg catgtatgta tatgcttcat
     1861 ttatctgatt cataaattct atatttccac ggatattatc tgccaacgcc tctgctttag
     1921 gattcaccac tccagatgtt tttaaaatat tttccatatt aattatctgt ttttcaattt
     1981 tatcgtacaa gccatcaatc ttcttaggat tcttttctaa atctcccggt tttatcatcc
     2041 attgctgttc taatgtctcc tttaacataa gcttaaaggc ttctcctgaa aacaactgtc
     2101 tcagtgcatc ggcatttgcc ggtgtgtttt ccaacatact atttatatca ttaagtattc
     2161 caacaacact gctatcctct gaatacaaag aaatattatt ttcctgtaaa tcaggaagca
     2221 gcttcctgac ctgttcatta agctcctcta tctgcttatc cgataaaata aatccaatag
     2281 tatttgattt tgcttcattc ccctgtaaaa atatattatc tattactgag gaattattta
     2341 ttttttgtgt tgtctgaata tcctgtgatt caccggaaac actgtttagt gattcgaata
     2401 attcagcaat attaaagtgc ttttttacta caggtgcaga ttgattatca cgcattatgg
     2461 cttcatactg agacatatct gcgtcttcat ttgaaataaa tgcatcttcc ggcaacccat
     2521 ctgtaacaat atttaagata tcccttgcct gacttacaag cttatacatt gataaatctt
     2581 cggctgaaag agcattcggc agttcctcta tcaaactatc catggcaatt gtaatagcct
     2641 gtttgtcatt agcataattt tcaaactgag ctgcgtttac ctgattaatt tcaattccaa
     2701 gcctctttaa ctcaaccagt gtagtcacat taatcccctt attatcctgc acaagctgat
     2761 acatctgatt aaggcttatc ttatcaatcg gcatctgctc ctccatcatc ttattcacca
     2821 tcgacaaatt ggtgccatct accggcagtc ctgcggcctt tagtgcatcc ataagtgtga
     2881 ggttgactcc ctctccatca acagtatatg gttttatttc tatagtgcct ccatcattat
     2941 ttttcacctg aaaaaacaca gactgcccct gttcaaatga aaacttccct gctaaccttg
     3001 ctaaaatctg ctgaccattt gacaaggcaa gcttaacctg atttccgcgg acactgctta
     3061 ctgttccctc aaatatcatc ccttttgaca tttcatttaa agaagatacc agcttctcaa
     3121 cgccctttgc tcctgtcata ggctcaccat ttgccactga attgttatat tgacttacca
     3181 aattagatat ctgcatagca tcaccctgat ataatatgat atgagggtca aaaggtattt
     3241 tacccctcat ttatgattaa gtatatattt ataaattgta tcatttattg gtatttctgt
     3301 cactatatta ctgttcaagc catactacga ccagcaacaa ttacaggctt tcaaaaatca
     3361 ggtaaattcc accatattct tcctatacca ctttggacaa agattgcgct gacattgtgg
     3421 attgaatcgt tcctttatat cagtgtactc aaaatacacc ttccataatt gttcaaaatc
     3481 atctgtcttc cccggcacac ttttctcaag ctgtttaaaa tcactttcat tgatataaaa
     3541 gcactcctta agcctcgggt gaaccactgc ataccttctt ctctcatcat agattacaaa
     3601 attctccccg ggatacctgt caacaaaatg ccacataaca ataggtatca tatcgcattt
     3661 cggctcaaac tgagccagca gatagtttcc attatcactg aaacgaataa aacctcttat
     3721 cttgtcagct tctttattta cctttcttga aag
//
LOCUS       DPUF01000243            3870 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_8831, whole
            genome shotgun sequence.
ACCESSION   DPUF01000243 DPUF01000000
VERSION     DPUF01000243.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_8831"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="DHV81_08365"
     CDS             complement(<1..119)
                     /locus_tag="DHV81_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCI94451.1"
                     /translation="MKKKVVVGMSGGVDSSVAAYLLKEQGYDVIGVTMQIWQD"
     gene            complement(119..565)
                     /gene="nifU"
                     /locus_tag="DHV81_08370"
     CDS             complement(119..565)
                     /gene="nifU"
                     /locus_tag="DHV81_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HCI94452.1"
                     /translation="MAYSEKVMDHFNHPRNVGEMENPSGVGTVGNAKCGDIMRMYLDI
                     DDNGIIQEAKFKTFGCGAAVATSSMATELVKGKTIQEALEVTNKAVMEALDGLPPVKV
                     HCSLLAEEAIHAALWDYAEKNGIKIEGLEKPVNDISEKEEAPEEDY"
     gene            complement(638..1831)
                     /gene="nifS"
                     /locus_tag="DHV81_08375"
     CDS             complement(638..1831)
                     /gene="nifS"
                     /locus_tag="DHV81_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HCI94453.1"
                     /translation="MSRLIYLDNAATTQVYPEVVDAMLPYFTEHYGNPSAIYSFADEA
                     ERAVDHARGEVADLIGAKKDEIYFTGGGSESDNWALKATAEAYAAKGKHIITSAIEHH
                     AILHTAQWLEKHGFEVTYVGVDEDGKIKLDELKAAIRPDTILISVMAANNEIGTIQPL
                     KEIGEIAKEHGILFHTDAVQAFGHIPINVDDMHIDMLSASGHKINGPKGIGVMYIRKG
                     VKIRSFIHGGAQERQRRAGTHNVPGIVGLGKAVELAKANMAERSAYETKLRDHLIERV
                     TSEIPYTRLNGHRTDRLPNNANFCFRFIEGESMLILLDQAGICGSSGSACTSGSLDPS
                     HVLLAIGLPHEIAHGSLRLTLSEKTTLEDIDYTVDKLKEIIARLRSMSPLYEDFVKKN
                     GKSEA"
     gene            complement(1851..2285)
                     /locus_tag="DHV81_08380"
     CDS             complement(1851..2285)
                     /locus_tag="DHV81_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCI94454.1"
                     /translation="MKISTKGRYALRLMMDLAENNTGSPISLKDVAKRQDISDKYLEQ
                     IISILNKAGYVRSVRGAQGGYMLKMEPQNYTVGMILRQTEGSLAPVACIEDDEIVCDR
                     QQQCVTSIVYKKINDAISGVVDNITLQDLVDWQNEKNGNYVI"
     assembly_gap    2378..2556
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2557..3206)
                     /locus_tag="DHV81_08385"
     CDS             complement(<2557..3206)
                     /locus_tag="DHV81_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCI94455.1"
                     /translation="MIQDIAPHTWDITYKNIQPDPDSRVLFFSRGQLLVQQASDKPAD
                     ESNQDIHQISFPRYEDIKTECPDAKYQYLFTLDDTAFFRVALSHADKMHILEVTGGKF
                     INRHYMRSAAPKEYVLAAITGFHLDGWYDKNHFCGRCANRLVEDDVERMLRCPVCGNM
                     VYPRINPAVIVGVTNGDKLLLTKYNGREYKKYALVAGFNEIGESLEETVRREVMEET"
     gene            complement(3291..3551)
                     /gene="ffs"
                     /locus_tag="DHV81_08390"
     ncRNA           complement(3291..3551)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="DHV81_08390"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          790 a          994 c          755 g         1152 t
ORIGIN      
        1 tcgtcctgcc atatctgcat tgtgactccg atgacatcgt atccctgttc ctttaataaa
       61 tatgccgcta ctgacgaatc cactccgccc gacattccta ctacaacctt ctttttcatt
      121 aatagtcttc ctcaggcgcc tcttccttct cgctgatatc gtttacaggc ttctcaagtc
      181 cctcgatctt gataccgttt ttctctgcgt aatcccagag tgctgcatgg attgcctcct
      241 ctgcgagaag tgagcagtgt acctttacag gtggaagtcc gtcaagtgcc tccataactg
      301 ccttgtttgt aacctcaagt gcctcctgga ttgtcttgcc ctttacgagc tctgttgcca
      361 tactgcttgt tgcaactgct gcgccacatc cgaaggtctt gaatttggcc tcctgaataa
      421 tgccgttgtc atctatatcc agatacattc tcatgatgtc tccgcacttt gcgtttccta
      481 ctgtgccgac accacttgga ttctccatct ctcctacgtt tcttggatgg ttaaaatggt
      541 ccattacctt ttcactgtat gccatgtcct tagttcctcc taattttcat gtatatatta
      601 atgttgtgat agtcactata atttctgacc tgatcattta tgcctcagat ttgccgtttt
      661 tctttacaaa atcctcgtat aacggtgaca tgcttctgag ccttgctatg atttccttta
      721 acttgtccac tgtatagtcg atatcctcaa gagtggtctt ctctgataat gtaagacgca
      781 gtgagccgtg tgctatctca tgtggcagtc ctattgcaag gagcacatgt gatgggtcga
      841 gtgagcctga tgtgcaggct gagcctgagc tgccgcatat gcctgcctgg tcaagcagga
      901 taagcattga ctctccctcg ataaatctga agcagaagtt tgcattgttt ggcagtctgt
      961 ctgttctgtg tccatttaaa cgtgtatatg gaatctcgct tgtcactctc tcgatgaggt
     1021 ggtctctgag ctttgtctca tatgcactgc gctctgccat gttggccttt gcaagctcta
     1081 ctgccttgcc gagtcctacg atacccggca cattatgtgt tcccgcacgt ctctggcgct
     1141 cctgtgctcc gccgtggata aatgagcgga tttttacgcc ctttctgata tacatcacgc
     1201 cgatgccctt tggtccgttg attttgtgac cgctggcgct gagcatgtcg atatgcatat
     1261 catctacatt aatcggaata tgtccgaatg cctgaaccgc atctgtatgg aacaaaatgc
     1321 catgctcctt tgctatctct ccgatctcct taagcggctg gatagttcca atctcgttgt
     1381 ttgccgccat aactgagata aggattgtgt caggtctgat ggctgccttc agctcatcaa
     1441 gcttgatttt tccgtcctca tcaacaccca catatgtcac ctcaaagcca tgcttctcaa
     1501 gccactgtgc tgtgtgaaga attgcatgat gctcgatggc tgatgtgatg atgtgctttc
     1561 cttttgcagc atatgcctct gctgtggcct ttaatgccca gttgtcggac tcgctgccgc
     1621 cgcctgtgaa atatatttca tccttcttgg ctccgataag gtctgcgacc tctcctctgg
     1681 catgatccac tgctctttct gcctcatctg caaagctgta tatagctgat ggattgccgt
     1741 aatgctctgt aaagtaagga agcattgcat ccacaacctc cggatatacc tgtgtggttg
     1801 ccgcattatc caaataaatt aatctgctca tctatgataa ccttctttct ttatatgaca
     1861 taatttccat tcttctcgtt ctgccagtct accagatcct gcaatgtgat attgtcaacc
     1921 acacctgaga ttgcatcatt gatcttcttg tacacaatag atgtgacgca ctgctgctgt
     1981 ctgtcgcaga ctatctcgtc atcctctata catgctaccg gcgcaaggct gccctcagtc
     2041 tgtctgagaa tcatgccgac tgtgtagttc tgcggttcca tcttcagcat gtatccgccc
     2101 tgggcgcctc tgacacttct gacgtagccc gccttgttta aaatggatat aatctgttca
     2161 agatatttat ctgaaatatc ctgtctcttc gctacatcct tcaggctgat gggtgaacca
     2221 gtgttgttct ctgcaagatc catcatgagg cgaagagcat aacgtccttt tgttgaaatt
     2281 ttcatttata gcacctctgt ttcatttcct actgttttac tatacattca gaaatcgcct
     2341 ttgtcaagcc gaaaaacaga aatcatttca tttgtcannn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttt cctccatgac ctcacggcgc
     2581 acagtctcct caagcgactc acctatctcg ttaaagcctg ccacaagtgc gtacttttta
     2641 tactctctgc cattgtattt tgtcaaaagc agcttatctc catttgtaac tcccactatc
     2701 actgccggat tgatgcgcgg gtaaaccatg ttgccacaga ccggacatct gagcatgcgc
     2761 tccacgtcgt cctctaccag cctgttggcg catctgccgc agaaatggtt cttatcatac
     2821 cagccatcca gatgaaatcc agttatcgcg gcaagcacgt actccttcgg tgccgcgctg
     2881 cgcatatagt gccggtttat gaatttgcca cctgttactt ccagtatatg cattttgtca
     2941 gcatgtgaca gtgccaccct gaaaaatgca gtatcgtcaa gtgtaaacag atactgatat
     3001 ttagcgtcgg ggcattctgt ttttatatcc tcatatcttg gaaagcttat ctgatgaata
     3061 tcctgattgg attcgtcagc aggcttgtcc gatgcctgct gcacaagaag ctgtcccctg
     3121 ctaaaaaaaa ggaccctgct gtccgggtcc ggctgtatgt ttttgtatgt tatgtcccac
     3181 gtatgtggtg caatgtcctg aatcattgta tgcctcgatt ttccttattt cgtgcgtata
     3241 aaccgtgcca ctacaaccga aaattcgcct cttttttata cgctaaaaat ccgagcgcct
     3301 cgcttcgaac catcacaaca gacgttacct ccacagttaa ctccctccag gctgcctcgc
     3361 gtcacatgaa agcttctact tagtgctgct gtgttcccac cctgacacgg ttcatagatt
     3421 tccattgcgc aagacccaga cgtcaacgcc acttatggag ggcagctcca ccgcttaagc
     3481 cctctacgaa gcatcacccc aactatagcg gattgttggt tcaaggtacc gctaccaccc
     3541 cggctgctcg gagtcttaag tatacgctat tatgcagggt tttgtcaaga tttattgcaa
     3601 cttcttgtgg gatgagtacg gcacgcaagc cccgggtacc ggacgcgcac acatactctc
     3661 ccgccgtcgc tacgctacgt ggctaagata ctgtacgaaa ttatgcttga gttttttcta
     3721 tacttgacga aaccgtcgaa acgcgccgtc catggcgcgg ttctcctcgc ccctgcgtcc
     3781 atgccgtggc gtttctgtgt ctcatgggca taatttctac agtatctaaa cctcttcgct
     3841 attcgcgctt cgggagagta cgtgtgagtc
//
LOCUS       DPUF01000244            9872 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7660, whole
            genome shotgun sequence.
ACCESSION   DPUF01000244 DPUF01000000
VERSION     DPUF01000244.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7660"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="DHV81_08395"
     CDS             complement(<1..431)
                     /locus_tag="DHV81_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HCI94456.1"
                     /translation="MKKKKNIIITAVMIVLIAIIAIILVKINSSNVKNKEAKGIKPGT
                     EAVKTKADTQAEDDNTTDKSGIEEFALFGVDSRSDQLDKGTRSDSIMVVRVDHDAKKI
                     RMVSIFRDCMMNIDGHGYQKVTHAHAFGGPELAVDTLNKNL"
     gene            complement(470..1195)
                     /locus_tag="DHV81_08400"
     CDS             complement(470..1195)
                     /locus_tag="DHV81_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCI94457.1"
                     /translation="METIVINQIKDQSYTMKESLRALKTSISFSGKGVKSILFTSSVP
                     NEGKTTVVMDLARSMADSKKSVLVVDTDMRKSVLVGRLRAKVESGGKIYGLSHYLSGQ
                     VSLDKVVYATNIKGVFILFAGHEVPNPTELLETEEFKELIEFGEKNFDYVLVDTPPTG
                     AAIDAAVVGKNVDGAVIVAAQNVTSSRAIINTKRTLEDSGVKILGAVLNKCRFEESKY
                     GHYYGNYYGNYYGNYYGRDEDEK"
     gene            complement(1195..1977)
                     /locus_tag="DHV81_08405"
     CDS             complement(1195..1977)
                     /locus_tag="DHV81_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCI94458.1"
                     /translation="MENNEPIRDQEIEIDLVALFHELVKHWKALVASMVLLAAVFGLY
                     SKITFVPEYEASAEMYVLTKSTSITSLADIQVGSSLTNDYEYVITGRTVLSQVIDNLD
                     MDETYEQLSKRVSIENPTDTRVLKIVVTDTDLEASKTVADEIAKVSSQYIADNMDQSQ
                     PKIIQTAYASKTPVNNNILKNTVIGAVLGLFLATGIVVLGYMLDDTISNADDMETRTG
                     LKVLASLPVDETEYDGAKTKRRKKKKTQSSKSDDSKSKKRAV"
     gene            complement(2206..4278)
                     /locus_tag="DHV81_08410"
     CDS             complement(2206..4278)
                     /locus_tag="DHV81_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94459.1"
                     /translation="MKKKSIKLLVVLACVLMALGMAGCGKKAYKTFPEKYKLASVDVG
                     GMTADEAKKAIKTAVGKYKISVKLDDTSFDMTAEDLGLKYNDKADLQELINAANKDKE
                     PEKQVKLFKMDKSDELETALVDSYITAKTQSQSDATAQSDTDTDANDDEQKKAATDTQ
                     TFDIKSIVPYRATIVYNAEAGQFEGVDGVSGDAPVYDNAAANLTSAVKELKDKVELTS
                     TTGYVDGEKATDSEQVKNALKEANAYLDVTVTCNFTPATGEAATEAVGKDQIAQWLIV
                     GNDGMSVSLDGENMATYCTELAKKHDVSKKKTGQFKTTGGSIINVPVASSGQTVDGNK
                     LYEAIAEAINNKKSATVEAVYSEAKEEETGEYVTYGGNYCEVDLTNQMVYVYKNGELI
                     VSSQCVTGCISKGHGTPTGVYSIFSRDKDRYLRGDGYKSWVNFFIPFNGGIGFHDASW
                     RSTFGGNIYLYSGSHGCINMPYSAAKKLYENVTLDEKVIVYGGVDKVAGKAQSLGGAD
                     SYNVTEGDGAFNLGVTAEDNAKLTYSSDNNGVVRVDENGNVTIAGVGTATITVKSEAT
                     TSYKAGSKTITITVNAKPQPNQTPSVTIGCGNKTVTEGDAPFSLGASASNGAALSYQS
                     SDPSIASVDGAGNVTIYRAGSVQITAIAGEMAGWNSASSSITVTVNSKPQPQPVAPTE
                     PSEQPQQN"
     gene            complement(4428..5489)
                     /locus_tag="DHV81_08415"
     CDS             complement(4428..5489)
                     /locus_tag="DHV81_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI94460.1"
                     /translation="METENKKILSIIIPTYNAAKFLDKGLSSFIIDDNSLLNMLDIIV
                     VNDGSTDNSVEIAQKYVNKYPDVYRILNKENGGHGSAINEGVKIIKGSYFKVVDADDW
                     VNTDVLKETICYLKDNENKHENFADAVLMSYRSYVLQRENTDEYPYDDKYITKNGGPY
                     NAGYVDEHLYDFWWGLSLHGVIYNTQFYRNTGLTLSEGVFYEDQEFSIIPMAYAKSIY
                     AYDKALYEYRVGDVNQSVSIESSLKRLDHYEKVIMRLIEAGRDAEHFSIGGKQLWFDK
                     TSKFINDYLQLCLIRNTDKKQLRKRMKLFVSQIEKKNTDMYKCVNKNYKVFCMLNKMH
                     MSDKVYQNYFIRLLHIVRH"
     gene            complement(5571..6698)
                     /locus_tag="DHV81_08420"
     CDS             complement(5571..6698)
                     /locus_tag="DHV81_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI94461.1"
                     /translation="MSKILSIIIPTYNAEKFLNKGLSSFLVCRDTDAQTAQHNCKMAT
                     EGRFEEIDIDKAILDKLEVIVVNDGTPDKSVEVAQKFVDWYPDTFVIVNKTNGGHGSA
                     INTGVEHVRGKYLKVVDADDWVDTLALKHLVEYLEHVDSDAVIQSFRYYDISKDEYRP
                     ADVSVPDFTREYNLKDIVNMWDDVYDGLSFHGVIYNTGFYKALGYKLTEHVFYEDQEY
                     ATVPFSYAKTVRFIQEELYVYRVGDVNQSVSAASQVKRLPDLEQVIFNVLEAEKSFAK
                     MPQGGKEHFIRKTSGIITSYYQIALIKNTDRQSGRAIVEQFNMKLAQKSALMYEAVQR
                     KYKVFRLFNKLHVSNSFYENQFAKLLELAKRVGRADRLYRK"
     gene            complement(6771..7766)
                     /locus_tag="DHV81_08425"
     CDS             complement(6771..7766)
                     /locus_tag="DHV81_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI94462.1"
                     /translation="MEKLLTIVVPAYNVEKYIKNCLDSFIDLSVLRSLEILIVDDGST
                     DSTASLAHTYEQKYPYSFKVLSKENGGHGSTINYAIPRATGKYFKVVDGDDWLDKSLL
                     PQFVQLLKHTHSDVISNDFNLVDDRTKKVTKRRKAVSNSYHYNREWGFAEAVMDPLIT
                     IHSMTIRTDVLQKNDIRVDEHCFYEDQEYILYPIPYCTSITFSPLPLYQYRLGRSGQS
                     VDIKMMIKRHDQHLKVLDALFEYNNVHGNLQTYKKQYLERGIAEAVDDEYQIFLAKGN
                     DTRNVEQMKKFDEMLREEHPGVYQACNRKSVWMLRKTGFKIFPMAAWVYSRLRGN"
     gene            complement(7802..8068)
                     /locus_tag="DHV81_08430"
     CDS             complement(7802..8068)
                     /locus_tag="DHV81_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCI94463.1"
                     /translation="MVAQKIIIKNPTGLHLRPAGNLCKEAMKFKSKVTFIYDEKNVAN
                     AKSVLSVLGACIKSGDEIELICNGEDESEALEHLIQYIKDGLGE"
     gene            complement(8099..9526)
                     /locus_tag="DHV81_08435"
     CDS             complement(8099..9526)
                     /locus_tag="DHV81_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94464.1"
                     /translation="MGKKLLKTFTNNIGFKILAIAFAFILWLVVYNLNDPVKTKTFTT
                     TVTVTGRDSVVDKGLWPTIKDSEKTISFSVSGKRSYLNELDDSDFYANVDLANIIVDK
                     DDTNKASVKVDIGCTKYRHSITFNGGDHMLPLSVEKYMQKQFEVKVSLDGSLSGAKAL
                     GNKPQANPKVVKIGGPESIVSTIASANVNIKVDDNTIISDNQITDRGDLTLIDDNGDE
                     IDISKLDVDSQYQSIAVTVDVLSTKEVPIKCTTTGSPAGGKSVLGVELSEESVMLKGN
                     AEALNNITSIDVGPIDISGATDDISTSVDLTGYLPDGVFIVNSSKAKLSIDIKIETNA
                     TSTMTLNSSNITYDGLEEGYTLTFVTDKSSVIVSGTKSDIDTLSGTTLKGKIDVTGLD
                     AGTHTVTVKPNLDETKYTWGEIKVQIVIGREGDGGGTTGTDGTGTASGSTTGDTTSGD
                     TGTGGSSGSTSSGNTGNNGSDSSSH"
     gene            complement(9492..>9872)
                     /locus_tag="DHV81_08440"
     CDS             complement(9492..>9872)
                     /locus_tag="DHV81_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCI94465.1"
                     /translation="SQLLINIFEKNTPLHDGAVIVRGNRVVSATCYLPLSDSLSLSKD
                     LGTRHRAAVGVSEVSDSLTIIVSEETGSVSTAYKGQIEHNIDADHLRTQLKLLQNRHR
                     EPGKFELIKRRFKNGKEASKNLHK"
BASE COUNT         2426 a         2407 c         1683 g         3356 t
ORIGIN      
        1 tccagatttt tgttaagtgt atccactgca agctccggtc cgccaaacgc atgtgcatgt
       61 gtcaccttct ggtatccatg accatcaata ttcatcatac agtctctgaa aatagatacc
      121 atgcggattt tcttagcatc atgatcaacc ctgactacca taatggaatc ggaacgtgtt
      181 cctttatcaa gctgatctga gcggctgtca acaccaaata acgcaaactc ctcaatacct
      241 gatttgtctg tagtgttgtc atcctcagcc tgagtatcag cttttgtctt aactgcctct
      301 gtacccggct ttatgccttt agcttcttta ttcttaacat tactgctgtt aatctttaca
      361 agtattatcg ctataatagc aataagtaca atcattactg ctgtaataat tatatttttc
      421 ttttttttca tctgcactat tctccgtcat tcatatgtgt atgtgcctgt tacttttcgt
      481 cctcatctct gccataataa ttgccatagt aattaccata atagtttcca tagtaatgac
      541 catacttaga ctcctcaaac ctgcacttat tgagcacagc tccaagaatc ttgactcccg
      601 agtcctcaag tgtacgcttt gtattaatga ttgcacggct gcttgtaaca ttctgggcag
      661 caactattac tgctccgtct acattctttc caactacggc cgcatcaatc gcagctcctg
      721 taggaggagt atcgacaagc acatagtcaa agttcttctc tccaaactcg ataagctctt
      781 taaactcctc tgtctcaaga agctctgtag gatttggtac ctcatgtcct gcaaagagaa
      841 taaacactcc tttgatgttt gtcgcatata ctactttgtc caaagatacc tgacctgaga
      901 gataatgtga gagtccgtat atctttccac cgctctccac tttcgctctg agtcgtccta
      961 caaggactga cttacgcata tcggtatcta ctacaaggac tgacttctta gagtccgcca
     1021 tcgaacgagc cagatccata actacggttg tctttccttc atttggcacg ctgctggtaa
     1081 agaggatgga cttcacgccc tttccactga aactgatgct tgttttaaga gctcgaagag
     1141 actccttcat tgtatagctc tggtctttta tctgattaat aactattgtt tccattaaac
     1201 tgccctcttc ttactcttgc tatcgtctga tttagatgat tgagttttct tcttttttct
     1261 tctctttgtc ttggctccat catactctgt ctcatcaaca ggaagtgatg caagcacctt
     1321 gaggcctgtt ctggtctcca tatcatctgc attggaaatt gtatcgtcaa gcatgtatcc
     1381 aagtacaact atgccggtag caagaaacag tccgagtaca gctcctataa ctgtattctt
     1441 aaggatatta ttattcaccg gtgtctttga agcatatgct gtctggataa tctttggctg
     1501 tgactgatcc atattgtcag cgatatactg tgatgaaacc tttgcaatct catctgccac
     1561 tgtctttgaa gcttccaaat cagtgtctgt aactacaatc ttgagcacac gtgtatctgt
     1621 cggattctct attgaaaccc tcttgctaag ctgctcgtat gtctcgtcca tatccagatt
     1681 gtcgattacc tgtgataaaa ctgtcctgcc tgtgataacg tactcatagt cgtttgtcag
     1741 actcgagcct acctgaatat cagcaagact ggtgatagat gttgacttgg tcagtacata
     1801 catctctgca gatgcctcat actccggtac aaaagtaatc ttggagtata atccaaatac
     1861 tgctgcaaga agcaccattg atgccacaag tgccttccag tgcttcacta attcgtgaaa
     1921 cagcgctact aaatcgattt caatttcctg atctcttatt ggttcgttgt tttccatttt
     1981 cctacccttt acaaattgta acattatttt tcggtttaat cctatgttat attactacac
     2041 ttcatttctt ttgtctattt ttagaagtca caaaaaaaat attaagaata tgtcatattt
     2101 tgtaaataat tatgcaatat aatagaaaag tgccgtttcc ggcacttttc tttgttgtat
     2161 tttcatttgc tatattttat ttgttcctgt tctattgttg ctgttctagt tctgctgtgg
     2221 ctgttctgat ggttcagtcg gtgccaccgg ctgtggttgt ggttttgaat ttactgtaac
     2281 agttattgaa ctgcttgcgc tattccagcc tgccatttca cctgctatag ctgtaatctg
     2341 tacgctacca gccctgtaaa tggtcacatt tccggcgcca tctactgacg caatcgaagg
     2401 atcacttgac tggtaagaaa gcgctgctcc gtttgaagca cttgctccga gagaaaatgg
     2461 tgcatctccc tctgttacag ttttattacc gcaacctatt gtcacagatg gtgtctggtt
     2521 tggctgtggc tttgcattta cagtgattgt aattgtcttg ctgccggcct tatagcttgt
     2581 agtagcctca gacttgacag tgattgttgc agttccgaca cctgctatcg tcacattacc
     2641 gttctcatcc actctcacta caccattatt atcacttgag tatgtgagct ttgcattatc
     2701 ctctgctgtc acaccgagat tgaatgctcc gtcgccctct gtcacattat aagagtcagc
     2761 tccgccaaga ctctgtgctt tgccggccac cttgtcaaca ccaccatata caattacttt
     2821 ttcatctaat gttacattct catatagctt ctttgcagct gaataaggca tatttatgca
     2881 gccatggctg ccgctgtata ggtagatatt tccaccgaaa gtacttctcc acgaagcatc
     2941 atggaaccca attccaccat tgaatggtat aaagaaatta acccatgact tgtatccgtc
     3001 tccccttaag tatctatcct tatctcttga gaagatagaa tacactccgg taggtgttcc
     3061 atgacctttt gaaatacatc ctgtaacaca ctgacttgat actataagct ctccattctt
     3121 gtatacatat accatctggt tggtaagatc cacttcacaa taattgccac catatgtgac
     3181 atattcacct gtttcttctt cctttgcctc actgtaaact gcttccactg ttgcagactt
     3241 tttattgttt attgcctcag caatcgcctc atacagctta tttccatcta cagtctgtcc
     3301 tgatgatgct acaggcacat ttattatact tccgcctgtt gtcttaaact gtcctgtttt
     3361 cttctttgac acatcatgct ttttggctag ctctgtacaa tatgtagcca tattctcgcc
     3421 atcaagcgaa actgacattc cgtcattgcc cactataagc cactgggcta tctgatcctt
     3481 tcctacagcc tctgtagcag cttcacctgt tgccggtgtg aaattacatg tcaccgtcac
     3541 atcaagatat gcattggctt cttttaatgc attctttacc tgctcgctgt ccgtagcctt
     3601 ttctccatca acatatcctg ttgtggatgt aagctccacc ttatccttca gctcctttac
     3661 agcacttgta agatttgcag ctgcattgtc atacacaggc gcatctcctg atacaccgtc
     3721 aacaccctca aattgtcctg cctctgcatt gtagactatt gtagcacgat atggcacaat
     3781 tgattttata tcaaaggtct gtgtgtctgt cgcagccttc ttttgttcgt catcatttgc
     3841 atctgtatcc gtatcgctct gagccgtagc atcagactgt gactgcgttt ttgcagtgat
     3901 ataagagtct acaagagccg tctccagctc atcagacttg tccattttaa aaagcttcac
     3961 ctgcttttcc ggttccttat ccttattggc cgcatttatc agttcctgaa gatctgcttt
     4021 gtcattgtac ttaagcccta aatcctctgc tgtcatatca aatgatgtat cgtcaagctt
     4081 cactgatatc ttatacttgc ctactgcagt ctttatggct ttctttgctt cgtcagcagt
     4141 cattccacct acatcaactg atgcaagctt gtacttttca gggaatgtct tgtatgcttt
     4201 ctttccacag cccgccattc ccagcgccat aagcacacat gccaaaacta ccaggagttt
     4261 tatacttttc ttcttcatct ttgtcctctt tatcttaatc ttaattttta tattattctc
     4321 acagcaccat attttatcac tttcttatat atgcctcaag tgagatttgt cacatatttt
     4381 ccgattattt aaacatatta ttcatcatta tgataacaaa aagcttccta atgacgcaca
     4441 atatgtaaca ggcgtataaa atagttctga taaaccttat cgctcatatg cattttattc
     4501 aacatacaaa atactttata attcttattt acacatttat acatatctgt attctttttt
     4561 tcgatttgac tgacaaacag cttcatcctt tttctaagct gttttttatc agtgtttcta
     4621 atcaggcaaa gctgcaaata atcgttgata aatttgcttg ttttatcaaa ccacagctgc
     4681 tttccgccta tactaaagtg ctccgcatcc cggcctgcct caatcagcct catgataacc
     4741 ttctcataat ggtcaagcct cttcagtgag ctttctatcg aaacgctctg atttacgtca
     4801 ccgactctat actcataaag tgccttatca tatgcataga tacttttggc atatgccatt
     4861 gggattattg aaaactcctg atcctcgtaa aataccccct cagagagtgt cagtcctgta
     4921 tttctataaa actgcgtatt atatatcacc ccatgcagac tcagccccca ccagaaatca
     4981 tacagatgct catccacata tcctgcatta tatggtccac cattttttgt tatatattta
     5041 tcgtcgtatg gatactcatc agtattttct ctctgcagca catatgatct atagctcatc
     5101 agcactgcat ctgcgaaatt ctcatgttta ttttcattat ctttaagata acaaatagtt
     5161 tctttcaaca catcagtgtt tacccagtca tcagcgtcta ctaccttaaa atagcttcct
     5221 tttattattt ttactccttc attaattgcc gagccatgac caccattttc tttattcagt
     5281 atccggtaca cgtcaggata tttatttaca tatttttgtg caatctccac actattatcg
     5341 gtagagccgt cattcacaac aataatgtcc agcatattta aaagagagtt atcatcaata
     5401 ataaatgatg ataacccttt atccaaaaac tttgccgcat tatatgtcgg tattattatt
     5461 gatagtatct ttttattttc tgtttccata tcctatctaa ttcctgtttc tatatgttaa
     5521 tttccactta tatcgctgtc tgtcaccata tttgacaatg gtagtcagta ttattttctg
     5581 tatagtctgt ctgctctgcc aacccttttg gcaagctcaa gcagctttgc aaattgattc
     5641 tcgtaaaagc tgttgctcac atgcagctta ttaaacagcc gaaatacttt atatttacgc
     5701 tgtactgcct catacataag tgcacttttt tgagcaagct tcatgttaaa ctgctctact
     5761 atcgctcttc cggattgtct gtcggtattt tttatcaaag ctatttgata atagcttgtg
     5821 attatgcccg acgtctttct gataaagtgt tcctttccac cctgtggcat ttttgcaaag
     5881 ctcttctctg cctcaagtac attaaatata acctgctcca aatccggaag acgcttcacc
     5941 tgactcgctg ctgaaacact ttgatttaca tctccaaccc tgtatacata caactcttcc
     6001 tgaataaatc tgacagtttt tgcatacgaa aacggaacgg ttgcatactc ctggtcttcg
     6061 taaaatacat gctctgtgag cttatatcca agtgctttat agaaccctgt gttatatatc
     6121 actccgtgaa atgatagacc atcatataca tcatcccaca tatttactat atcctttaaa
     6181 ttgtactctc ttgtaaaatc aggcacactc acatctgccg gtctgtactc atcctttgat
     6241 atgtcataat acctgaagct ttgtatcacc gcatccgaat ctacatgctc gagatattca
     6301 accaggtgct ttagcgcaag cgtatctacc cagtcatctg catcgaccac ctttaaatac
     6361 tttcctctca catgctccac gcctgtgttg attgcagaac cgtgtccacc atttgtttta
     6421 tttactatga caaaggtatc cggataccag tccacaaatt tctgcgccac ttcaacactt
     6481 ttgtcaggtg tgccgtcatt tacgactatc acctcaagct tatcgagtat agccttgtca
     6541 atatctattt cctcaaagcg gccctccgtt gccattttac agttgtgctg tgctgtctgt
     6601 gcatctgtat ccctgcatac cagaaatgac gacagcccct tattcagaaa cttttctgca
     6661 ttatatgttg gtattatgat tgataatatt ttactcatta actgttctat ccgtcctgat
     6721 tactattatt aaaaagagta ttgcatactc attaacgcct attcatgaaa ttaattacca
     6781 cgcagtctgc tatagaccca tgctgccatc ggaaatatct taaagcctgt ttttctaagc
     6841 atccagacgc tcttcctgtt gcacgcctgg tacactcccg ggtgctcctc acgcagcatc
     6901 tcgtcaaact tcttcatctg ctcaacgttc cttgtatcat tgccctttgc aagaaaaatc
     6961 tggtactcat catcgactgc ctcggctata ccgcgctcaa ggtactgctt cttgtaggtc
     7021 tgcagattgc cgtgcacgtt gttgtactca aacagtgcat caagcacctt aaggtgctgg
     7081 tcatgacgct tgatcatcat ctttatgtcc acgctctggc cactccttcc aagtctgtac
     7141 tgatacagcg gcagtggtga aaacgtgatg cttgtgcagt atggtatagg gtagagtata
     7201 tactcctggt cctcgtagaa gcagtgctca tccacacgaa tatcattctt ctgcaataca
     7261 tcagtacgga tggtcatgga atgtatagtg atcagcggat ccatgactgc ctcagcaaag
     7321 ccccactccc tgttgtagtg ataagagttg gacactgcct ttctgcgctt tgtgaccttc
     7381 tttgtcctat catccaccag gttgaagtca ttggatatga catccgagtg cgtgtgcttc
     7441 aaaagctgta caaattgtgg caaaaggctc ttgtccagcc agtcatcacc atccaccacc
     7501 ttgaaatact ttcctgttgc acgcggtatc gcatagttga tggtagatcc atggcctccg
     7561 ttctcctttg agagcacctt gaagctgtat ggatatttct gctcgtaggt atgtgccagg
     7621 ctggctgtgg aatctgtgga accatcatcc actatgagta tctccagact tctgagcaca
     7681 gacaaatcta taaacgagtc aagacagttc tttatatact tttcaacatt gtaagccgga
     7741 acgactatgg ttaaaagctt ctccatatat gtttctccat gtatacctgt ttccttgtgt
     7801 actactctcc gagtccatcc ttgatgtact gtataaggtg ttcaagtgcc tcactctcat
     7861 cctcgccatt gcagataagt tctatctcat cgcctgactt tatgcatgct ccaagcactg
     7921 aaagtacact ctttgcatta gctacatttt tctcgtcata tataaatgtt acctttgatt
     7981 taaatttcat tgcctctttg cagagattgc cggccggacg taaatgtaat ccggtaggat
     8041 tcttgattat tattttttgt gctaccactt ttattacctc ttttcggtta tatatctatc
     8101 aatgtgatga actgtcagag ccgttatttc cggtgtttcc tgacgatgtg ctgcccgatg
     8161 aaccacctgt gccggtgtcg cctgatgttg tatcgcctgt tgttgagccc gaggctgttc
     8221 ctgtaccatc agttcccgtt gtgccgcctc cgtcaccttc ccttccgata actatctgaa
     8281 cctttatctc gccccatgtg tacttggtct catccagatt tggctttacc gtcactgtat
     8341 gtgtacctgc atcaaggccg gtcacatcaa ttttaccctt cagtgtagtt cccgaaagtg
     8401 tatctatatc agacttggtg ccgcttacta tgacactgct cttatctgtt acaaatgtaa
     8461 gcgtatatcc ctcttcaagt ccgtcatatg tgatattgga gctgttcagc gtcatagttg
     8521 aagtcgcatt tgtttcaatc tttatatcta tactgagctt tgccttggat gagttcacta
     8581 taaaaactcc atccggcaga tatcctgtca ggtcaacaga tgtacttata tcgtctgttg
     8641 cacccgatat atcgattggg cccacatcta tagatgtgat attgttcaat gcttctgcat
     8701 tacctttgag cattacagac tcctctgata gttctactcc gagcacgctc tttcctcctg
     8761 ccggtgagcc tgtcgtagta cacttgatag gcacctcctt ggtacttagg acatcaactg
     8821 tcactgctat agactgatac tggctgtcca catcaagctt tgatatatct atctcatcgc
     8881 cattatcatc aatcagagtg agatcaccgc gatcagttat ctgattatct gatattatgg
     8941 tattgtcatc gacctttata tttacattgg ccgatgctat agttgaaaca atggactcag
     9001 gacctccgat ctttactacc tttggatttg cctgtggctt gtttcccagt gctttcgcac
     9061 ctgagagact tccatccaat gataccttta cctcaaactg cttctgcata tatttttcaa
     9121 ccgaaagcgg gagcatatgg tctccaccgt tgaaggtaat cgaatgtctg tacttggtgc
     9181 atccaatatc tacctttacg cttgccttat tcgtatcatc cttatctact atgatatttg
     9241 caaggtcgac atttgcgtaa aaatcagaat catcaagctc gttcagatag cttctcttgc
     9301 ctgataccga aaagctgatg gtcttttccg aatccttgat ggtaggccat agccccttgt
     9361 caacaaccga atcacggcct gtgacggtga ctgttgtggt aaatgtcttt gtcttgaccg
     9421 ggtcattgag attgtagacc acaagccaga gtataaatgc aaatgctatc gcaagtattt
     9481 taaagcctat attatttgtg aaggttttta gaagcttctt tcccattctt aaacctcctt
     9541 ttaattaatt caaatttacc cggctcccta tgtctgttct gcagaagctt aagctgtgtg
     9601 cgtagatgat cggcatctat attgtgctca atctgcccct tgtatgctgt agaaactgat
     9661 cctgtctcct ctgaaactat gatagtaagc gaatcactta cctcgctgac tccgaccgct
     9721 gcacggtgtc tggttcccaa atccttcgag agtgacaggc tgtctgatag cggaaggtaa
     9781 catgtagctg acaccacacg gttgccacgc acgataaccg ctccgtcatg aagcggtgtg
     9841 tttttctcaa aaatattgat gaggagctga ct
//
LOCUS       DPUF01000245            5154 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13044, whole
            genome shotgun sequence.
ACCESSION   DPUF01000245 DPUF01000000
VERSION     DPUF01000245.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13044"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="DHV81_08445"
     CDS             complement(<1..1484)
                     /locus_tag="DHV81_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCI94466.1"
                     /translation="MSSKKDAMKKKLENMHLGKRINYGYKTVITMMLISGLLSIAVIG
                     VLFFNMLNYVNKVNAADQAVKTCRINVNAAARNVREMALNPDKSTYDDYEQGAKEKLE
                     DVDVQLKTIKKSGIVPEDQYNEYSKALSDWANIGYSIMDKIKAGQKDEAVDQIINECT
                     PKLNKTVELAKEINKLTDERSHQAVVRTYVCAAVGLIVIIICLICAWQLTKRTGKIVL
                     DTILEPLHAIEEVAKELTEGNLHSTLDYRSEDEIGRLAHSMRKSIRILGSYVDDIDRS
                     MKLFADGHFDAQPEVEWRGDFVGILHSFMEFEKSMAVTIKGIQNVSSEVSSAAEQVAA
                     SSNDLADGATNQAAVVEELTATVEGVSEQVEQNSKSAKEISGRVDELGGAIWESNGKM
                     QEMVTSMHEINEASKQIDQIISTINEIASQTNLLALNASIEAARAGEAGKGFAVVANQ
                     VNMLADQSAQAAKESAALIETSVQAVEKGMNIAEQTASQLEEVA"
     gene            complement(1782..2558)
                     /locus_tag="DHV81_08450"
     CDS             complement(1782..2558)
                     /locus_tag="DHV81_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCI94467.1"
                     /translation="MSTLITIIVTFFAGMGAGLGTGFAGLSAAAVISPMLITFLGMDP
                     YMAVGIALSSDVLASAVSAYTYGKNKNLDVKNGLIMMVTVLCFTVVGSYVSSLVPSAT
                     MGNFSVFMTFLLGIKFIVRPVMTTKEAMQGVSAKKRAVQSIICGVLIGFICGFIGAGG
                     GMMMLLILTSVLGYELKTAVGTSVFIMAFTAFTGAASHFAIGGKPDWFVLILCIAFTL
                     LWARIAAVFANKASAKTLNRATGIVLVILGIVVFAFSLLS"
     gene            complement(2555..2995)
                     /locus_tag="DHV81_08455"
     CDS             complement(2555..2995)
                     /locus_tag="DHV81_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCI94468.1"
                     /translation="MKFNFEFAEKISRTYSDECRPLCHELKLPQTAFDILMFLANNPE
                     YKTARDIVEIRKIKANLVSVNVDKLVNEGYLERKPVENDRRKTELVCTKKAGTVIERG
                     RRIQQQFTANLFAGIDEEQKKQLTIVMEQLENNMDEIIRRKSHK"
     gene            3351..4748
                     /locus_tag="DHV81_08460"
     CDS             3351..4748
                     /locus_tag="DHV81_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI94469.1"
                     /translation="MSQETNSAPVTENKMGTMPIGRLVFNMSLPMMISMLVQALYNIV
                     DSIFVAKLSENALTAVSLAFPLQTLIIAVATGTGVGVNALLSKSLGAHKYDEANKIGI
                     NGAVLYAFSYVIFLILGLTIVKPFYMSQIGNADIEIMDMGVSYLSTVMIFSFGIFAEI
                     FFERVLTSTGRTIFSMTSQLCGAITNIILDPIMIFGLLGFPKLGATGAAVATVVGQCV
                     AAVVAFVCNHKFNHEISLSFKGYSPDFKIMGTIYAIGIPSIIMQSIGSIMTYAMNRIL
                     IGFSSTAAAVFGVYFKLQSFFFMPVFGLNNGITPIIAFNYGAQNRKRMVKTIKVSLIT
                     AFCLLFVGFACFEFIPHVLLGMFSASDDMLKIGVPALRIIGVHFLIAWFCIIVGTVFQ
                     ALGKAVFSMIVSIMRQLVVLIPAAYILSRLGGLHAVWWAFPIAEVISFTVSTLFLIRI
                     NRTIISKIPDGSDVL"
BASE COUNT         1350 a         1180 c          925 g         1699 t
ORIGIN      
        1 tctgctactt cttcaagctg tgaagctgtc tgctctgcaa tattcatgcc tttttcaaca
       61 gcctgcacag atgtctctat aagcgcagct gattcctttg cagcctgtgc actttgatcg
      121 gcaagcatat ttacctgatt tgccacaacc gcaaagcctt ttcctgcctc ccctgctctt
      181 gctgcctcaa tagaagcgtt taatgcaagc aggttggtct gtgatgcaat ctcattaatg
      241 gttgatataa tctgatctat ctgtttagag gcttcgttta tttcatgcat agatgtaacc
      301 atctcctgca tctttccatt gctttcccaa attgcacctc caagctcatc cactcttcca
      361 ctgatttcct ttgcactctt tgaattctgc tccacctgct ctgaaactcc ctcaactgtc
      421 gctgtcagtt cctctacaac cgcagcctga ttggttgcac cgtctgcaag gtcatttgag
      481 cttgcagcta cctgctctgc cgcacttgaa acctcgcttg atacattctg gattcccttt
      541 atggtgactg ccatactttt ttcaaattcc ataaatgaat gtaaaatacc tacaaaatcg
      601 cctctccact ctacttccgg ctgtgcatca aaatgtccgt ccgcaaagag cttcatcgac
      661 ctgtctatat catccacata tgagccgaga atacggatag actttctcat gctgtgtgcc
      721 aatctaccga tttcatcctc tgaacggtaa tcaagtgtgc tgtgaagatt tccttctgtc
      781 agctcctttg ccacttcctc gattgcatgt aatggctcaa ggattgtatc aagcacgatc
      841 tttcctgttc tctttgtcaa ctgccatgca catatcagac agataatgat gactatgagt
      901 ccgactgcag cacatacata tgtccttaca actgcctgat ggcttctttc gtcagtcagc
      961 ttgttgattt cctttgcaag ctcaactgtt ttatttaact ttggtgtaca ctcattgata
     1021 atctgatcaa cagcctcatc cttctgtccg gctttaatct tatccatgat tgagtatccg
     1081 atattcgccc agtcagaaag agcctttgaa tattcattat actgatcctc aggaacgata
     1141 cctgattttt ttattgtttt taactgcaca tcaacatctt caagcttttc ttttgcgccc
     1201 tgctcatagt cgtcatatgt tgatttgtca ggattaagtg ccatctctct tacatttcta
     1261 gccgctgcat ttacatttat tctacatgtc ttaaccgcct gatcggcagc atttaccttg
     1321 tttacatagt ttaacatatt gaaaaacaac acaccaatga ccgcaatcga aagcagtccc
     1381 gaaataagca tcatggtaat aaccgttttg taaccatagt tgatcctttt acccaaatgc
     1441 atgttttcca atttcttttt catcgcatct ttttttgaag acatagtttg ttatttcctc
     1501 ctatcttatg ataccttttg accctttaat catattatta tttatactca agtctgaatt
     1561 cgtcagatga atttagtata gtactatttt tctaagggca caatactgta ttctcaataa
     1621 tctttatgtg aatctttcca ttaaatatgt ggatttgcac aaaatggtag tttttttttc
     1681 gcaatcccct taaaatacat atgatgattt tttcttgttt tatgtaatga tgtaagcccc
     1741 ggtcgggcag tcggggctta cagttatata ttcaagaaaa atcagctcag aagtgaaaat
     1801 gcaaatacca cgatgccaag tattacaagc acaatacctg tggcacgatt taaggttttt
     1861 gcggatgcct tgtttgcaaa gaccgctgca attctcgccc aaaggagtgt aaatgcaata
     1921 cataaaatca gcacaaacca gtccggtttt ccaccgatgg caaagtgcga ggcagctcct
     1981 gtaaaggctg taaatgccat gataaatacg ctcgttccga ctgcagtctt aagctcatag
     2041 ccaagcaccg atgtgaggat gaggagcatc atcataccgc ctcccgcacc tatgaagcca
     2101 cagataaatc cgataagcac gccgcagatg attgactgga cagctctctt tttagcagaa
     2161 actccctgca tggcctcctt tgttgtcatg acaggacgga ctataaactt tattccgagt
     2221 aaaaatgtca taaataccga aaagttgccc attgttgctg acggcacaag gcttgataca
     2281 tagcttccaa caaccgtgaa gcataaaacc gtgaccatca ttatgagccc gttttttaca
     2341 tcaaggttct tgttctttcc atatgtatag gctgacactg cgctggcaag tacatcagac
     2401 gacagggcta ttcccactgc catataagga tccattccca aaaatgtaat gagcataggg
     2461 cttattactg ctgctgcgct tagtcccgca aagccagttc caaggcctgc gcccataccg
     2521 gcaaaaaatg ttactattat agttattaat gtgctcattt gtgtgatttc ctcctgatta
     2581 tttcatccat attattctca agctgttcca taacaatcgt cagctgcttt ttctgctcct
     2641 catctatccc tgcaaacaga tttgctgtaa actgctgctg tatacgcctt cctctttcaa
     2701 tgacagtccc ggcctttttt gtacagacaa gctccgtctt cctcctatca ttttccacag
     2761 gttttctctc cagatagcct tcattcacaa gcttatcaac atttacagac acaagatttg
     2821 ccttgatttt tcttatttca actatatccc ttgccgtctt atattccgga ttgttcgcca
     2881 gaaacatgag tatatcaaag gctgtctgcg gaagcttcag ctcatggcac agtggtctgc
     2941 actcatcgct gtaggttcgc gaaatcttct ctgcaaactc aaagttaaat ttcatatgtg
     3001 atattccttc ttttcttttc ctattttgct gcttcgttat acatttttga attattcatt
     3061 tttgaactaa ttatatatct ctctgtcatt ttgtcaagcg gttatcgtaa tttataccct
     3121 gcaatgctat actttttgag gcattttttc attaactatt atgatatgat ttaacagtta
     3181 tataatacct tgaaagtatc ctgcagtcat ggtattatat gtaattggcg ctttgttgca
     3241 cattttaaac accggctaat ctaccggtga ttttaatttt aataactaaa gcttcgtaca
     3301 tatataatgt aggagtttta aattaacaat catttacaat aggaggaatt atgagtcagg
     3361 aaacaaattc agcccctgta acagagaaca agatgggaac catgcctatc ggcagacttg
     3421 tatttaacat gtctttgcct atgatgattt ccatgctcgt acaggctcta tacaacatcg
     3481 tggacagtat tttcgttgca aagctgtccg aaaacgcact cacagcagtt tcacttgcct
     3541 tccctttgca gacactcatc atcgctgtcg caaccggtac cggtgtaggt gtaaacgcgc
     3601 tgctgtcaaa gtcactcggc gcccataaat acgacgaggc caacaaaatc ggcatcaacg
     3661 gtgccgtact atatgcattc agctatgtaa tatttctgat tctcggactt acaatcgtaa
     3721 agccttttta catgagtcag attggaaatg ctgatatcga aatcatggat atgggtgtga
     3781 gctacctgag tactgttatg attttttcat ttggtatttt tgcggaaatc ttttttgaga
     3841 gggtgcttac ctcgacagga agaactattt tcagcatgac ctcacagctt tgcggtgcca
     3901 tcacaaatat cattctggat cctatcatga tctttggact gcttggcttc cctaagctcg
     3961 gtgctaccgg tgcagccgtg gcaaccgttg tgggacagtg cgtagccgcc gttgttgcct
     4021 ttgtctgcaa ccacaaattc aatcatgaaa tcagcctgag cttcaagggc tacagcccgg
     4081 atttcaaaat catgggaacc atttatgcta tcggtattcc gtcgatcatc atgcagtcta
     4141 taggctctat aatgacatat gccatgaaca gaatacttat cggcttttca tcgactgccg
     4201 ctgctgtttt cggtgtttat ttcaaactgc agagcttctt ctttatgcct gtattcggac
     4261 tgaacaacgg aatcacacct atcatcgcct tcaactacgg tgcacagaac agaaagcgta
     4321 tggttaagac aataaaggtc agcctgataa cagcattctg tctgctcttc gttggctttg
     4381 cctgctttga gttcatacca catgtgcttc ttggaatgtt cagcgcctct gacgatatgc
     4441 tcaaaatcgg tgttccggcg ctcagaataa taggagtgca tttcctcatc gcatggtttt
     4501 gtattatcgt cggcaccgta ttccaggctt tgggaaaggc tgtcttttcc atgatcgtat
     4561 caatcatgcg tcagcttgtg gttttaattc cggcagcata catcctttca aggctcggtg
     4621 gacttcacgc tgtatggtgg gctttcccta tagcagaggt gatatccttt actgtttcga
     4681 cattattctt aatcaggatc aacagaacta tcatctcaaa gattcctgat ggaagcgatg
     4741 tgctgtaaaa aattttactt gagctttaaa ataatcaaca ataaaatccg ctgcattata
     4801 tcatgtgcgt aatcatatga tatagtgtag cggatttatt atatttatgt catattttca
     4861 catatttctg atttctgcac cagcttggtg cagcttatga accgactcca tcttgcgtat
     4921 tatcttgcgt attttcttcc tctattatac gtattttatg ttgctggtca cactgttcgg
     4981 tgtgaacagt aactatttta tataaaaagt gtaatctgca gattcaaacc tcttttaaaa
     5041 aatgcgattt tgtggtaaaa tagcaaaaaa gaaaatgcga ttttgtggta aatcgttgaa
     5101 ataaaaaatg cgattttgta gtaaaacatc aaaataaaaa atgcgatttt gtaa
//
LOCUS       DPUF01000246            9115 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1428, whole
            genome shotgun sequence.
ACCESSION   DPUF01000246 DPUF01000000
VERSION     DPUF01000246.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1428"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="proB"
                     /locus_tag="DHV81_08465"
     CDS             <1..849
                     /gene="proB"
                     /locus_tag="DHV81_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCI94470.1"
                     /translation="DNYKRVVIKIGSSTLTHAETGSLNFSKMERLVRSICDYRNSGMD
                     VCLVSSGAIAVGRDVIGIKERPSDISIKQACAAVGQGRLMMTYQKLFSEYNQNSGQVL
                     MTKNTIVNPVSRRNVMNTFEELFDLNVVPIVNENDTVSTYEMQFGDNDTLSAIVTSIT
                     KADLLILLSDIDGLYSDDPHDNPDAKLIREVDTLDRKILGMAKSSTGSDVGTGGMATK
                     LTAAKIATYSGADMIIANGGNMGILYDIMNDRYTGTLFHRAKDAEFVLADYIQREMAP
                     TSTGGQ"
     gene            881..1105
                     /locus_tag="DHV81_08470"
     CDS             881..1105
                     /locus_tag="DHV81_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCI94471.1"
                     /translation="MTQEKIQRINELYRKSQAEGLSEAEKKEQDLLRKEYIANVKKNL
                     RNQLNNIDMVNDDGSVENLGEKYGNKKRNS"
     gene            1080..1655
                     /locus_tag="DHV81_08475"
     CDS             1080..1655
                     /locus_tag="DHV81_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCI94472.1"
                     /translation="METKKEIRSRLKKQRSLLCADESHSMSHDIYKRLMVSELDRDYD
                     NILLYSAIRNEVNTDEYFTYLINKAKRIYYPRVSGNKMSFYRVRSLDELNCGSFNIKE
                     PDMTKEYTQADGRALMIVPGLAFSDTGYRIGYGKGFYDRYLSSFTKRDTVMAVGVGYD
                     FQLLSSMTFEDEYDVPLDGVITDKREVFIDE"
     gene            1648..2904
                     /locus_tag="DHV81_08480"
     CDS             1648..2904
                     /locus_tag="DHV81_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCI94473.1"
                     /translation="MNDLEKICADAYETRVKIGTLDTDIKNKVLNDAADNLLKAEKEI
                     LEANKRDVATAEENMKAKSMIDRLSLDHDRLLGMADGLRQIAKLADPIGEVMSMSKRP
                     NGLIIGKRRVAIGVVGIIFEARPNVTSDAFGLCFKTGNCVILKGGSDAINTNIAIVKA
                     LKKALTDNLVSDAALALIESTDRETTNAFMKMDQYVDVLIPRGGAGLIQNVVKNATIP
                     VIQTGTGNCHVYVDKDADFDMAVNIINNAKTQRISVCNACESIVVHSAIAEEFLPKLY
                     DKLREHHVQLRCDERAQAILAGRDDVTEATADDWGMEYLDYIMSVKIVDSIDEAIEHI
                     NRYNTSHSEAIVTNDYDNAQKFLNEIDAACVYVNASTRFSDGNEFGFGAEIGISTQKL
                     HARGPMGLEALTSYKYIIYGSGQIRE"
     gene            2952..4391
                     /gene="miaB"
                     /locus_tag="DHV81_08485"
     CDS             2952..4391
                     /gene="miaB"
                     /locus_tag="DHV81_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCI94474.1"
                     /translation="MSYDIDITKTPPAHEPERQYYYMAKAKDFVEKKSKEIGRPMTYF
                     VKTFGCQMNARDSEKLVGILEQIGYVEGTDEHSDFIVYNTCTVRENANNKVYGRLGYL
                     QNYKKKNPLMKIALCGCMMQEPEVVENIKKHYKFVDIVFGTHNIFKFAEILCNNIESG
                     SQVIDIWKDTNQIVEDLPVKRKFSFKSGVNIMFGCNNFCSYCIVPYVRGRERSREPKD
                     IIREIEKLVADGVCEIMLLGQNVNSYGKTLDNPITFAELLREVNKIEGLKRIRFMTSH
                     PKDLSDDLIMAIKECDKVCKHMHLPLQSGSSRVLKEMNRHYDKEKYLDEVKRLREQIP
                     DIAITTDIIVGFPGETEEDFLETMDVVKQVRYDSAFTFIYSKRTGTRAATMENQVPDD
                     VVKDRFDRLLKEVQTISSEKAKCYEGKVVPVLAEEMDDQKEGYVTGRMDNNSIVHFPG
                     TEDMIGNIYNVKLDECRGFYYMGEIKEDA"
     gene            4384..4782
                     /locus_tag="DHV81_08490"
     CDS             4384..4782
                     /locus_tag="DHV81_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCI94475.1"
                     /translation="MRKRFGRNDIILISVIVIISVALLLIWKLVYLKGQDTDSDACVE
                     VTIDGRAYGTYPLSKDDTIEIKNGDGDVTNTLVIKGGVADMTSADCPDHLCVKQKAIS
                     KEGESIICLPNKVVVTVKSDTKSDIDSISK"
     gene            4816..5313
                     /locus_tag="DHV81_08495"
     CDS             4816..5313
                     /locus_tag="DHV81_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase"
                     /protein_id="HCI94476.1"
                     /translation="MKTRKIAYLGMLMALALVLSYVESLIPFAVGIPGVKLGLTNIVT
                     VIMLYIAAPMETFLLCVFRAVLSGFMFGNAFSIIYSLAGCILSFIVMYLLKKSDKFSS
                     VSISLVGGVCHNIGQIIVAAIVLSTYSVVYYIPVLLVAGCITGLVIGIVASQVLLRIS
                     DSFHV"
     gene            5408..6025
                     /locus_tag="DHV81_08500"
     CDS             5408..6025
                     /locus_tag="DHV81_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCI94477.1"
                     /translation="MDSMYAYIKGELAEKNIDSIVVEAAGVGYLIYIPTQYFDMLPDE
                     GEDVKIYTYLCVREDAMILYGFLSKDDLEIFKMLITVSGIGPKGGLAILSTLPADDLR
                     FAILSGDSKAISKAPGIGAKTAQRVILDLKDKLSLEDAFEKKLENQASGAAVSMNSTV
                     KNDAVMALNALGYSSTESLKAVSKVDITEDMDVEDVLKLALKNMG"
     gene            6072..7091
                     /locus_tag="DHV81_08505"
                     /pseudo
     CDS             6072..7091
                     /locus_tag="DHV81_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882908.1"
                     /note="too many ambiguous residues; incomplete; partial in
                     the middle of a contig; missing start; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
     assembly_gap    6653..6746
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7196..7669
                     /locus_tag="DHV81_08510"
     CDS             7196..7669
                     /locus_tag="DHV81_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCI94478.1"
                     /translation="MSAKTNAEVVIDGKVYTLSGYENEEYLQKVAAYINNKIAEFDDM
                     DEYKHLPGNMKSTLIELNIADDYFKAKALVEKLEGDIENKDKEIYDLKHDLISNQVKT
                     ENAEASMKELEAQNKELLLNKARLEATLEESLLDGKKPVKKSAHASKGANDSSDK"
     gene            7758..>8461
                     /locus_tag="DHV81_08515"
     CDS             7758..>8461
                     /locus_tag="DHV81_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32"
                     /protein_id="HCI94479.1"
                     /translation="MRNKDFELLAPAGNLEILKGVIESGADAVYVGGSMFGARAYANN
                     FTEEELLEAIDFAHLRGVKVYLTVNTLIKNSEFSKLYDYLLVYYKRGLDAVIVQDLGV
                     VKAIHEYFPSMEIHTSTQMTVTGADGVRFLSQFGVTRVVMAREVSLAEMKRIHEETGM
                     ELEAFVHGALCYSYSGQCLFSSILGGRSGNRGRCAQPCRLPYTVEGKKDEYILSLKDM
                     CGIKALDKLHDAGVYSL"
     assembly_gap    8462..8569
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8570..>9115
                     /locus_tag="DHV81_08520"
     CDS             <8570..>9115
                     /locus_tag="DHV81_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32"
                     /protein_id="HCI94480.1"
                     /translation="DYDRIKALGNRCGFTDRYYFDHNGSDMVTYVKPNFVSNAAEPSP
                     EKRKLSIEGELVLREGEPGSLTVKRGDVTYKASIESVSAALKAPLDKKAAIDRINKTG
                     DTDFEFSHIKAQIGENVFVPNGALNKLRRDAISGLCDKLLEKYYRNDARYADMSRLTA
                     LPEHVVKSDAAHDEAINDYTTI"
BASE COUNT         2809 a         1537 c         2282 g         2285 t
ORIGIN      
        1 gacaattata aaagagtggt aataaagata ggttcatcga cgctgacaca tgcagagacg
       61 ggaagcctca atttttcaaa gatggagcgt ctggtgcgct cgatatgtga ttacagaaac
      121 agcggtatgg atgtgtgcct cgtaagctcc ggcgctatcg cggttggacg tgatgtgata
      181 ggcataaagg agcgcccgag cgacatttca ataaagcagg catgcgccgc agttggacag
      241 ggcaggctta tgatgacata tcagaagctt ttttccgagt ataatcagaa ttccggacag
      301 gtgctcatga caaagaatac catagtcaat cctgtttcaa gacgcaatgt tatgaataca
      361 tttgaggagc tttttgatct gaacgtggtg cctattgtca atgaaaatga tacggtaagc
      421 acatacgaga tgcagtttgg tgacaatgat acgctgtctg ctattgttac atcaatcacg
      481 aaggccgatc tgctcatact gctttctgat atagacggac tgtattccga cgatcctcat
      541 gacaatccgg atgcaaagct tatcagagag gttgatacgc ttgacaggaa aatacttggc
      601 atggcaaagt catcaaccgg cagcgatgtc ggaaccggcg gcatggcgac aaagcttaca
      661 gcggcaaaga ttgccacata ttccggtgcc gatatgatta tcgcaaacgg cggcaatatg
      721 ggaattttat atgacattat gaatgacaga tatacaggaa cacttttcca cagggcaaag
      781 gatgcagagt ttgtgcttgc ggattatata caaagagaga tggcacctac atctacaggc
      841 ggccagtaat cagctataaa tttgaaatca ggagagtgct atgacacagg aaaaaataca
      901 gcgaatcaat gagctttaca gaaagtcaca ggcagaggga ctttctgagg cagagaaaaa
      961 agagcaggat cttttgcgca aggagtatat agcaaacgtg aagaagaatt taagaaacca
     1021 gctcaacaat atcgatatgg taaacgatga cggttcggtt gagaacctcg gcgagaaata
     1081 tggaaacaaa aaaagaaatt cgtagcaggc taaaaaagca aaggagcctt ttgtgtgctg
     1141 atgagagcca cagtatgagc catgacattt ataaaagact catggtatca gagcttgaca
     1201 gagattatga caacatactt ttgtattcag ctatcagaaa tgaagtgaat actgatgagt
     1261 attttaccta tttaataaac aaagcaaaaa ggatatatta tccgagggtt tcgggcaata
     1321 aaatgtcctt ttacagggta agaagccttg atgagcttaa ttgtggttca tttaatatta
     1381 aagagcctga tatgacgaag gagtatacgc aggcggatgg cagggcgctt atgatagtgc
     1441 cggggcttgc attttcggat accggttaca gaatcggcta tggaaagggc ttttatgaca
     1501 gatatttatc gtcttttaca aaaagagaca ctgtcatggc agttggagtg ggctacgatt
     1561 tccagttact tagcagcatg acgtttgaag atgaatatga cgtaccctta gatggggtga
     1621 taacagataa gagagaggtg tttattgatg aatgatttag aaaaaatttg tgcggatgcg
     1681 tatgagacca gagtaaagat aggaacactg gatacggata tcaagaataa ggtattaaac
     1741 gatgcagcgg acaatcttct taaggcagaa aaagaaatcc ttgaggcaaa caaaagggat
     1801 gtggccacag cagaggaaaa tatgaaggca aagagcatga ttgacaggct ctcgcttgat
     1861 catgacaggc tccttggcat ggctgacgga ctcagacaga tagcaaagct tgcagaccca
     1921 attggggaag tgatgtctat gtcaaagcgt cctaatggtc ttattatcgg aaaaagaaga
     1981 gttgcaatcg gtgttgttgg cattatcttt gaggcgcgac caaatgtcac atcagatgct
     2041 tttggtctgt gctttaagac aggcaactgt gtcatattga agggtggaag tgatgcgata
     2101 aataccaata tcgcaatagt taaggcatta aaaaaggctt taacggacaa tttggtttct
     2161 gatgctgcac ttgcacttat tgagtcaaca gacagagaga caacaaatgc gtttatgaag
     2221 atggatcagt atgtggatgt gctcatacca agaggtggtg caggactcat ccagaatgtt
     2281 gtcaaaaatg ctacaattcc tgtcatccag accggtacag gcaactgcca tgtatatgtg
     2341 gacaaggatg cagactttga tatggctgtt aatattatta acaatgcaaa gacacaaaga
     2401 atcagtgtct gcaatgcatg tgagtcgatt gtagtacaca gcgccatagc agaggaattt
     2461 ttgccaaagc tgtatgataa gctaagagag catcatgtac agctgcgttg tgatgaaaga
     2521 gcgcaggcaa ttcttgccgg aagagatgat gtgacagagg ccactgccga tgactggggt
     2581 atggagtacc ttgattacat catgtcggta aagattgttg acagtattga tgaggcaatc
     2641 gagcatatca acagatacaa cacatcacat tctgaggcaa tcgtcacaaa tgattatgac
     2701 aacgcacaga agttcttaaa tgagatagat gctgcatgcg tttatgtaaa tgcttcaacc
     2761 agattttctg atggaaatga attcggcttt ggagctgaga taggaatcag cacacagaag
     2821 cttcacgcaa ggggacctat ggggcttgag gcactgacaa gctataagta tattatttac
     2881 ggcagtggtc agatcaggga atagtcagct aaagtatcat agaattaatt tgagaggaaa
     2941 cataatataa catgagctac gatatagata taacaaagac accaccggca catgagcctg
     3001 agaggcagta ttattacatg gcaaaggcaa aggattttgt tgaaaagaaa tctaaagaaa
     3061 tcggccgtcc tatgacttat tttgtaaaga cattcggctg tcagatgaat gcccgagact
     3121 cagaaaagct tgtcggaata ctcgagcaga ttggctatgt ggaaggaact gatgagcact
     3181 cggattttat tgtatacaac acctgtacag tacgtgagaa tgctaacaat aaggtgtatg
     3241 gcaggcttgg atatctgcag aactacaaga aaaagaatcc tctgatgaag attgctctct
     3301 gcggctgcat gatgcaggag ccggaggttg ttgaaaatat caaaaagcac tataagtttg
     3361 tagatatcgt attcggaacc cacaatatat tcaagttcgc agagatactg tgcaacaata
     3421 tcgagtctgg ctcacaggtg attgacatat ggaaggatac caatcagatt gtcgaagatc
     3481 tgccggtgaa gagaaagttc tctttcaaat caggtgtcaa tatcatgttt ggctgcaaca
     3541 atttctgcag ctactgtatc gtgccatatg tgagaggacg tgagagaagc agggagccta
     3601 aggatatcat aagagagata gaaaagcttg ttgcagacgg agtgtgcgag ataatgcttc
     3661 tcggacagaa tgttaactca tacggtaaaa ctcttgacaa tcctataacc tttgcagagc
     3721 ttcttcgcga ggtcaataag attgagggac ttaagaggat tcgttttatg acatctcatc
     3781 caaaggattt atcagatgac ctgattatgg cgataaagga gtgcgacaag gtgtgcaagc
     3841 atatgcatct gccgcttcag tcaggaagct caagagtgct taaggagatg aacaggcact
     3901 atgacaagga aaaatatctc gacgaggtaa agagacttcg tgagcagatt cctgatattg
     3961 caatcacaac cgatatcatt gtgggcttcc cgggagagac tgaggaggat ttccttgaga
     4021 caatggatgt tgttaagcag gtcagatatg acagtgcttt tacatttatt tactcaaaga
     4081 gaacaggcac acgcgcagcc acaatggaga atcaggtgcc ggatgatgtg gtcaaggaca
     4141 ggtttgacag gctcttaaag gaggttcaga caatctcatc agaaaaggca aaatgttatg
     4201 agggtaaagt ggtgccggta cttgcagagg agatggatga ccagaaggag ggctatgtga
     4261 ccggacgtat ggacaataat tcaatcgtac attttccggg gaccgaggat atgattggaa
     4321 atatctacaa tgtgaagctt gatgagtgca gaggctttta ctatatggga gagataaaag
     4381 aggatgcgta agcgttttgg cagaaatgat ataatcctga tatcggttat tgtaattatc
     4441 tctgtcgctc tgcttcttat atggaagctt gtatatttaa aggggcagga tacggatagc
     4501 gatgcatgtg tcgaggtgac tattgatgga agggcctatg gcacatatcc gctttcaaag
     4561 gatgatacaa ttgagataaa aaacggcgat ggcgatgtta ctaacaccct tgtcataaag
     4621 ggtggtgtgg cggatatgac ctctgccgac tgtccggatc atctgtgtgt gaagcagaag
     4681 gcgatatcca aggagggaga gtcgattatc tgtctgccaa ataaggttgt tgtgacggta
     4741 aaaagcgaca cgaaatccga tatagattcg atatccaagt gatacccgaa catttttata
     4801 gaaagaatga gataaatgaa aaccagaaag attgcatatc ttggtatgct tatggcactg
     4861 gcactggtgc taagctatgt tgagtcactt ataccgtttg cggtaggcat accgggagtg
     4921 aagcttggac ttacaaatat agtgacagta atcatgcttt atatagcagc acccatggag
     4981 acgtttctgc tatgtgtatt tcgcgcggtg ctttcgggct ttatgtttgg aaatgcattc
     5041 agcatcatat attcactggc aggctgtata ctaagcttta tcgttatgta cctgcttaag
     5101 aaatcagata aattcagttc ggtttcaata agcctggtcg gtggagtgtg tcacaatata
     5161 ggccagataa tagttgcggc tatagttttg tcaacctaca gcgtagtgta ttatattccg
     5221 gtgcttcttg tggcaggatg catcacggga cttgtgattg gaatagtagc ttcacaggtg
     5281 cttttaagga tttctgacag ctttcatgta tagttttaat gggctgtaat ctacgtatat
     5341 ttaataacaa aaggagatgc tgctgttttt atgcctgtgt gtcaggctat agacgaaaca
     5401 gtaacaaatg gattcaatgt atgcatatat aaaaggagag cttgcagaaa aaaatataga
     5461 cagcattgta gtggaggcag caggtgtagg ctatctcatt tacataccga cgcagtattt
     5521 tgatatgctg cctgacgagg gagaggatgt aaaaatttac acctacctgt gtgtcaggga
     5581 ggatgccatg atactttacg gttttttgag caaggatgat cttgaaatct ttaagatgct
     5641 gattacagtc agcggaatcg gaccaaaggg cggacttgcc attttgtcaa ctctgcctgc
     5701 cgatgatttg cgttttgcga ttctatcggg cgattcaaag gctatatcaa aggcaccggg
     5761 aataggtgca aagacggctc agagagtgat acttgattta aaggacaagc tgtcgcttga
     5821 ggatgcattt gaaaagaagc ttgagaatca ggcatccggt gctgctgtat ccatgaacag
     5881 cactgtgaaa aatgatgctg tcatggcgct caatgcgctt ggatactcat ctactgagag
     5941 tcttaaggca gtgtccaagg ttgatatcac agaggatatg gatgtagagg atgtgctgaa
     6001 gcttgcgctc aaaaatatgg gctgattgat aaaagctgta agcttaaaac agaaacgggg
     6061 aacaaaacgt gaaaagagta atttccacac aattgcagga agaggacata aaaatagaaa
     6121 aatccctgcg tcctatgtat tttgatgact atatcggtca gcagaagata aaggacaatt
     6181 taaaaatata tattgaagcc gcaaaatcaa ggggcgaggc gctcgatcat gtgctgtttt
     6241 acggacctcc ggggcttgga aagacaacac ttgcaggaat cattgcaaat gagatgggca
     6301 caaagataaa ggtgacctcc ggtcctgcaa taggtaagcc gggagagatg gcagccatac
     6361 tcaacggact ttctgaggga gatatactgt ttgtcgatga gattcacaga ttaaaccgtc
     6421 aggtagagga ggtgctctat cctgccatgg aggattacgc cattgatatc atgattggta
     6481 agggcgagtc tgcaaagtca atcagattta acctgccaaa attcacactt gtaggtgcaa
     6541 caacgagggc gggtatgctt tcagcgcctc ttcgggacag gtttggcgtg gtaaatcaca
     6601 tggagtttta cactgtcgat gagctaaagc atattatcgt caattccgca aannnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnccaa gacttgcaaa cagactgcta aagcgtgtca
     6781 gggattttgc gcaggtcaag tatgacggca aaatcaccta tgatgtggca agctttgcac
     6841 tggatctttt ggaagtggac aagtacggac ttgatttaaa cgacagaaat atacttctca
     6901 ccatcattga caagtttgcg ggaggaccgg tgggtcttga tacgcttgca gcggcaatag
     6961 gtgaggatgc cggaaccatt gaggatgttt acgagccgta tctggtgaag aacggcttca
     7021 taaaccgcac accaaaagga cgtgtggcta cagaactagc ctttgagcat tttgagagaa
     7081 aaagggctta aagttgcaat ttatatctat aaggcatata ataattaagt atttgatatt
     7141 gaaattgata ttaatatagc tgatttgaat aaatgtgaaa acggaggatg attaaatgtc
     7201 tgcaaaaaca aatgccgagg tggtaataga cggaaaggtt tacacactgt caggatatga
     7261 gaatgaggaa tacttacaga aggttgcggc atacatcaac aacaagattg cagagtttga
     7321 tgatatggat gagtataagc atcttccggg caatatgaag agcacactta tcgagctcaa
     7381 tattgcggat gattatttca aggcaaaggc acttgtcgaa aagctcgagg gtgatataga
     7441 gaataaggat aaggaaatat acgatttaaa gcatgattta atttccaatc aggtgaagac
     7501 agagaatgcc gaggcgagca tgaaggagct tgaggcacaa aacaaggagc ttttactcaa
     7561 caaggcgaga cttgaggcaa ctcttgagga gtcacttctt gatggaaaaa agcctgttaa
     7621 aaaatctgca catgcttcta aaggggcaaa tgacagtagc gataaatagc aataaaatgg
     7681 gaacagttat cgtacactgt tccctatttt attgcaggtt tatatgatat caaatgagca
     7741 tctatatgag gaaatatatg agaaataaag attttgaact gcttgcaccg gcaggaaacc
     7801 ttgagatact taagggtgtg atagaaagcg gcgccgatgc tgtgtatgtg ggcggaagca
     7861 tgtttggagc cagggcatat gccaataatt tcacagaaga ggagctttta gaggcaattg
     7921 attttgcgca tttacgagga gtaaaggtgt atctgacggt caatacgctg attaaaaaca
     7981 gtgagttttc aaagctctac gactacctgc ttgtgtacta caaaagaggg cttgatgcag
     8041 tgatagtgca ggaccttggc gtggtgaagg ctatccatga gtattttccg tcaatggaga
     8101 tacatacgag tacacagatg acggtaacgg gagcagacgg agtgcgcttt ctttcgcagt
     8161 ttggagtgac gcgtgtggtc atggcaaggg aggtttctct tgccgagatg aagcgcatac
     8221 atgaggaaac cggcatggag cttgaagcct ttgtgcacgg agcattgtgc tattcgtatt
     8281 ccggacagtg ccttttcagc agtattttag gtggtagaag cggaaacaga ggccgctgtg
     8341 cacagccgtg cagactgccg tataccgtgg agggcaaaaa ggatgaatac attttaagcc
     8401 ttaaggatat gtgtggtata aaggctcttg acaagctgca tgatgcaggt gtttattcgc
     8461 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng attatgacag
     8581 gataaaggca cttggaaacc gctgtggttt cacagacagg tattatttcg accacaatgg
     8641 ctctgatatg gtgacttatg tgaagcctaa tttcgtttca aatgctgcag agccttcgcc
     8701 tgaaaaaaga aagctttcga tagagggaga gcttgtttta agagagggtg agcccggaag
     8761 tctcactgta aaaagaggtg atgtgacata caaagcttca atagaatctg tcagcgccgc
     8821 acttaaggca ccgcttgata aaaaagccgc tatcgacagg ataaataaaa ccggtgatac
     8881 agactttgag ttttcacata ttaaagctca aatcggagaa aacgtctttg tgccaaacgg
     8941 agcactaaac aagctgcggc gtgatgcaat ttcagggctt tgtgataagc tattggaaaa
     9001 gtattacagg aatgatgcac gatacgctga tatgtcaaga cttacagcac ttcctgagca
     9061 tgtcgtaaaa agtgatgcag cacatgatga agctataaat gattacacca ccata
//
LOCUS       DPUF01000247            5369 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13697, whole
            genome shotgun sequence.
ACCESSION   DPUF01000247 DPUF01000000
VERSION     DPUF01000247.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13697"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            394..1218
                     /locus_tag="DHV81_08525"
                     /pseudo
     CDS             394..1218
                     /locus_tag="DHV81_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516980.1"
                     /note="too many ambiguous residues; incomplete; partial in
                     the middle of a contig; missing start; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    753..801
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1215..1871)
                     /locus_tag="DHV81_08530"
     CDS             complement(1215..1871)
                     /locus_tag="DHV81_08530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94481.1"
                     /translation="MLMTKKQAIAIITKCAKQYQQYLEGNQVVFVYRDENNKSNHTAV
                     RFHSHNFLHFTGVTPRTGMNANGFYRAALNNRLSENDFSFKSNHTTELKLKVLGIIMS
                     MDTSARMIGNYTGPHLELYTEKVTGTTTACLGLIQSKDCYIPNSVLSEDIRSIVPKPP
                     GKIFAIFKKPIGAPLYTQLTYKSKNISITKKCLPKELLTEVDTSLLEDNNNSDDNEPA
                     "
     gene            2225..2611
                     /locus_tag="DHV81_08535"
     CDS             2225..2611
                     /locus_tag="DHV81_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HCI94482.1"
                     /translation="MVIHTKEKAKIHSHEPKGARIKGSNIYTVERGPKIAGAKAAETM
                     GKKSYRKSTVYQVNVKEKGLSRFKRNVREAKHIYEVTETEVVNPYDNSIKTQSTEKEL
                     TLFTCSQKGTMRFVVRCIYKEAVMDE"
     gene            2604..2900
                     /locus_tag="DHV81_08540"
                     /pseudo
     CDS             2604..2900
                     /locus_tag="DHV81_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2943..3260
                     /locus_tag="DHV81_08545"
     CDS             2943..3260
                     /locus_tag="DHV81_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94483.1"
                     /translation="MKTVKKMLVSMELHLDASIDKLDNLAMNVQLDKEKHMENAKTKE
                     QTEPERAEAERVEAEIVYAPMVAEPQEYQYNADAFEARGVDEVKQEAAHKEVSKVRAD
                     KAR"
     gene            4606..5304
                     /locus_tag="DHV81_08550"
     CDS             4606..5304
                     /locus_tag="DHV81_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94484.1"
                     /translation="MKKIIRILFVCLFFIALIFIGKNVLKDGKEREYIKSDKYYEENI
                     ENININEVNFKFLQVNEVANVNEAFYKKYDSYKEVVVEDNKNYDVFEKYTKVDVAFDQ
                     GYSGMRCYLNEDESFGGAYFRYTFEYETNDENLVDSSIEVYITAEPLEKFEGLLPTIE
                     EADEGNVYSIYDNILYYMENDDNMSLFKTNICTVVFDGSKYSYFCGVIDGEKESYINK
                     YCNVLYNFFQQMYK"
BASE COUNT         1754 a          803 c         1202 g         1561 t
ORIGIN      
        1 actttctcaa agttataccg caagtgctat atgtagcagt atatctttaa tgggtgtatt
       61 tgtttcagta gtgccattat ttgtatatga gtgcacagga ttaattcaga cggcagttct
      121 ggcaaggcta caatattcat ttagtggaag atatgctgta cgaacgggaa tatatataga
      181 taacatgtaa aaaataggat taattagagt tatataaaga aagagcactt tagccaaaga
      241 aatttaggtt aaggtgcttt ttttatgccc taaaaggagg tggtcacagc agcaacacac
      301 atcagggaaa gcctgactaa ttttagttcc gggagtaatc ccgtttatta tagaaacaat
      361 tatataatgc agataagaat ggatgaatga catcttgatc cgaatgattc caagggaatg
      421 gtaagagaaa agggtgagtt catgcttggt ctttgtgagc agtgtatcgg agacagctta
      481 aattcaagac agaagtcaat cattgaccgc tgtgtaagaa agctgtatat cgacattgca
      541 agaagcaaag agaagtatat tccggtgatg agcgattttt acgatatcct tatggcacag
      601 ccggaggacg aggcaaagga cattgcattg tctttggagc ttttcgtaaa tggttcgctt
      661 aacatcttca atcaccagac aaatgtggat gtagacaaca gattcacagt ttatggtatc
      721 agagatcttg gtacggagct tagtcctatc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nacatggctc tatattgatg agtttcacgt cttacttaat
      841 tccgagtatt ctgcaaaata tctgcagcag ctctggaaga aagtaagaaa gcagggaggt
      901 ctttgtactg gtatcacgca gaacgtagta gatctgttgc agaactatac agcaacaaca
      961 atgcttgcaa actcagagtt tgtagcactc ttaaaacagg cgaatacaga cagttccaag
     1021 atggcggaag ttatcggggt atcagaggca cagctaagat tcgtaaccaa taccgcatcg
     1081 ggaatgggac ttattaagtg tggttctgtg gtgattccgt ttgataatca gattagcaag
     1141 gatacagatt tgtacaagct gtacaatacc aacattcacg aaaaaatcga gatgatgaaa
     1201 aagcaggctg aaaattaagc cggctcgttg tcatctgaat tattattgtc ttctaaaaga
     1261 gatgtatcaa cttcagtgag cagctctttt ggaaggcatt tctttgtaat acttatattt
     1321 tttgatttgt atgtaagctg tgtatatagt ggtgcaccaa ttggtttttt aaagattgca
     1381 aatatctttc ctggcggttt tggaactatg gaacggatat cttcacttaa cacgctgtta
     1441 ggtatatagc agtctttgct ctgaatcaga ccaagacatg cagtagtagt tccggttact
     1501 ttctcagtgt atagctcaag gtgtggtccg gtatagttgc ctatcatacg tgcagaggta
     1561 tccatactca tgataatacc aagtactttg agttttaatt ctgttgtatg gttgcttttg
     1621 aaggaaaaat cattttcact taacctatta ttcagggcag cacgatagaa gccatttgca
     1681 ttcattcctg ttcttggagt gactcctgta aagtggagaa agttatgcga atgaaatctt
     1741 acagcagtat ggtttgactt attgttttcg tcacgataga caaaaacaac ctgattacct
     1801 tctaaatact gttggtattg ttttgcacac ttggtgatga tggcaatagc ctgtttctta
     1861 gtcataagca ttaaggtcct tatatttaaa tggtattttg gcaaatttaa aaggaaggtt
     1921 cttctgccgt agcagatgac accttccttt aatgactgat ttttctgttg tccgccaact
     1981 gatcggcacc cgaagtccga taagaaaact ggtttttccg ttgccagcca actgtccggc
     2041 acccgaagtc cggaaagaat ttcggatttt atgtgtcagt ccacatgata ggcatatagc
     2101 ccatctacta actatagtat acacagataa agcaaattat gcaacgagtt tataaagatt
     2161 taatcagaaa tttttggaaa agaaggtgag aacattgaag ataaaaaagg tggatgataa
     2221 gcctatggtc attcacacaa aagagaaagc caagattcat tctcatgagc ccaaaggagc
     2281 gaggataaag ggcagtaaca tctatacagt agaacgtggt cctaagattg ctggagcaaa
     2341 agcagcagaa acaatgggta agaaatcata caggaagagt acagtctatc aagtaaatgt
     2401 aaaagagaaa ggtctttcaa gatttaagcg taatgtaagg gaagccaagc atatctatga
     2461 ggtaacagag acagaggtag taaatccgta tgataacagc atcaagacac agagtacaga
     2521 aaaagaatta actcttttta cctgttccca gaaaggaacc atgcgttttg tggtcaggtg
     2581 tatttacaag gaggcggtga tggatgaata agagataccg gttaggtgag atagaagagg
     2641 cagtatcgga aatggaggag ttaatcgggc ttgaggatga tattgcagag attgatgatg
     2701 actttcagat agtcgtaagc ggctggtctg tgtatgtcga aagcctgaat ctgacactca
     2761 gacagggaat agcctgtgta tgggatgcgg aggaaggatt atttatgcct gattttgatg
     2821 tgaccatcgt gtatgagggg aacattgaaa cacaggagtg gctctactac gagcaggatg
     2881 gaatggtcgt tacacttggc aaagagcaga aacatcccat tacaaaggca gtacttgccc
     2941 ctatgaaaac agtgaaaaag atgcttgtat caatggaact tcatcttgat gcttcgattg
     3001 ataagctgga taatctggct atgaatgtgc agcttgataa ggaaaagcat atggagaatg
     3061 cgaaaacaaa ggaacagaca gagccggaga gggcagaagc tgagcgagta gaagcagaga
     3121 ttgtatatgc accaatggtc gctgaaccac aggagtatca gtataatgca gatgcatttg
     3181 aggcaagagg tgtggatgag gtgaagcagg aagcagcaca taaggaagta tcgaaggtaa
     3241 gagcagataa agccagatag tagtgagggc gtgggagaga ttctgcgtcc ttttttacac
     3301 cgatttgttg caatacagaa cattaattcg atgtacaagg gatgattatt gtacagttta
     3361 aagtgctata aataaagcat aaggaaagca taagtgacgg atgcatgata aatgagttta
     3421 atagaacatt tacggaggtg gcagttatgc atatattggt gtttatttta atatttgttg
     3481 ttggttcaat cattgccgct tgtgatgggg attattcatg aatagctgca atcggtatat
     3541 ttgtaggatt ttttgtattg ctattgcagt attatggtta tttacccagc ctgttttatt
     3601 aataattgct attaggctat tattgaattc cgatgtgcaa acttcatttt ccgatgagct
     3661 tccagtttgt cagggtttgt gatatactcc tttatggatt gaatgatatt tttgcatatg
     3721 tcggttcgat tcataagaca tacctcctaa gtgtgattaa gtttgtcgct gcaacgacct
     3781 cacttttgga ggcttttgtc aagtcttttc agaaaaaaac agaaattttc acaaaaaaat
     3841 aaagccctat agccttgaaa aatcaaggct ttggggctta agttgacaac attgccgcct
     3901 gatactgaaa ttcaccgtaa tagaccgcat aggaaaccat atcatctttt gttatttcag
     3961 cgagctgttc atctatatga gatgccagct tgcgaaggtt gccattttct tttaagcgaa
     4021 tggcatgaaa aatatctggc ataatgcaaa actaagatga cgtggttaca gtagagccaa
     4081 ttaaatcgtg agctatgcag agaaaagtgt gggattaacg gatggaacgg atgcaggccg
     4141 tgaagccgta aaatggtatg gagtccggaa cggactgcat ctgcaaaaga ccaacaagaa
     4201 cctgagatgg tatagcgtcc actgagtaaa tcagtaccac gacaggtgaa tgcaggtcag
     4261 taacaatcaa acataaaaac aagagctgta gccggcatcg tttttttccg tggggcatgc
     4321 ccccgtttag gagcttggct ggcactcagt gaatggtaga tgctacgaag gaagttggga
     4381 catgatcccc ttagacacgg aaggttcatc atcatagttg aacagaggga aaatagcctt
     4441 tataaagcaa actaaaagga tgctttatta gctatactta tttatcagct attcggtaca
     4501 tgacactacg atatccatca ctctcggctt ttgtttgagg taaaataaaa atcgtcaaag
     4561 ccagtaaaat caatagattt aggattgaca gaataggaga aatgaatgaa gaaaataata
     4621 agaatattat ttgtatgctt gttttttatt gcattaattt ttataggcaa gaatgtatta
     4681 aaggatggca aagaacggga atatataaag tctgacaagt attatgagga aaacatagaa
     4741 aatataaata taaatgaagt taacttcaaa tttctccaag taaatgaagt tgctaatgtc
     4801 aatgaagcat tttataagaa atatgattct tataaagaag tagtggtgga agataacaag
     4861 aattatgatg tttttgaaaa atatacgaag gtagatgttg catttgacca aggatattct
     4921 ggaatgcgat gttacttaaa tgaagatgaa agtttcggtg gagcttattt ccgatataca
     4981 tttgaatatg aaacaaatga cgaaaatctt gtggattctt ctattgaagt atatataacg
     5041 gctgaaccgt tagaaaagtt tgaaggttta ttaccaacta ttgaagaggc tgatgaaggg
     5101 aatgtatata gtatatatga taacattctc tattatatgg aaaatgatga taatatgagc
     5161 ttatttaaaa ctaatatatg tacagttgtt tttgatggtt ctaagtacag ttatttttgt
     5221 ggtgttatag atggcgagaa agaatcttat atcaataaat attgtaatgt tttatataac
     5281 ttttttcaac aaatgtataa atgataaaac tataaaaatt atagtaattt atgaaatgaa
     5341 tctacaagac atatagtatt tcataaatt
//
LOCUS       DPUF01000248            7885 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7264, whole
            genome shotgun sequence.
ACCESSION   DPUF01000248 DPUF01000000
VERSION     DPUF01000248.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7264"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="DHV81_08555"
     CDS             <1..1314
                     /locus_tag="DHV81_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HCI94485.1"
                     /translation="PELDKQGLQELLAIGPAHTSGLSLFKDLFEVLPGHFMIYSQDGL
                     HDETYWELTSREHTESYRDTVAHTRFLVEDAIKRQMVSDVPVCTFLSGGIDSSIVTAI
                     AANYMNTRGKTLNTFSFDFKDNDRYFKANAFQPERDLPYVNRMLEEYETAHSYLECDE
                     NSLFKALFAAVDAKDMPGMTDVDSSLLYFCSLVSRKNKVALTGECADEIFGGYPWFYR
                     KELLERYGFPWSSDVAPRLALLRDDVGFELDLSGYQAFRYEESRSKAPLLYGEAGEDE
                     SRRIIGYLNIKWFMQTLLDRMDRTSMYSELEARVPFADHRIIEYVFNVPWHMKYQNGV
                     EKTLLRDAFSDVLPPELLHRKKSPYPKTYHPGYEALLIKGMEEILDSPNAPVRALIDT
                     GKTREFLKAPAEYGRPWFGQLMAGPQLIAYFIQINYWMEKYQLSV"
     gene            complement(1311..3353)
                     /locus_tag="DHV81_08560"
     CDS             complement(1311..3353)
                     /locus_tag="DHV81_08560"
                     /inference="COORDINATES: protein motif:HMM:PF06964.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HCI94486.1"
                     /translation="MQITDKRGVKITPEMIGLFFEDINFAADGGLYAEMIENRSFEAK
                     EGFGTPGNFYSVDDNGYAWEPYAADVQDKPRMQYIMGTPLSEANPHYLRFTATEAGQG
                     FSNKAYDGIRLHKGMKYNVSFYARCVDYAGSDFVISVNKDGKTYAETTVEAVKAIPYV
                     PFSDLKIEMEQKDPVMCAQLEKLKKLDTDKMCRCNDWIKYDAVLTADDDVAGAKFTIA
                     LDGEGVIEFDLISMIPEDAVKGIFRRDLYEALAAIKPGFIRFPGGCIVEGISLENRYR
                     WKRTVGELKDRKYIPNLWAFDDDRSKTGWDVKRPDSHYGQSFGIGFYEYFLLCELLGA
                     KALPVLSIGTACQFRSTQLVTSDSEEFDEYVQDALDLIEFANGPVNSKWGALRAKMGH
                     PESFGLDMISVGNEQWESQYVDLYKRHQIFEKAIHEKYPDMRILGTAGPFLDSPITED
                     AWNFYNSEFKANPNTCYAVDEHYYVSPQWMYDHVDHYDDYPRDIAVFAGEYAAHTTDR
                     QNNMESALAEAALLTGIEKNADVVKLASYAPLFNRVGHSQWKPDMIWFDDEKVYLTPN
                     YYVQMLFANHVGDETIPMNGQEKELRKDGIYVSLSRTQQGETILKVANVNDSDYTLPL
                     TDGADVPVEAIGHAWILEGTGEIPEGMPEITDVRETNVQIIGNVTVLAQSFMVLRF"
     gene            complement(3408..4523)
                     /locus_tag="DHV81_08565"
     CDS             complement(3408..4523)
                     /locus_tag="DHV81_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94487.1"
                     /translation="MSGRKRQKTGMIDNENNSMAQETQIPQQTVDAQQVVIPQESERI
                     QRAEWIHEQVKLQREAEDEAWEKIMEEQKLLTERRRSIDADINSNKELKRYHEDCMED
                     ITDQVYSLHGISVDKLEGIKEYKNALFRGEAFIVFCISVIMAAIVAYAYGPTSQLCLY
                     ILMCVGAEGALLSQKRKNILFFIPLILIAAEMIGYKLYPQYLEYVTIVGEALALLLMM
                     IGTVGYFVHNPYRKDRKNIKAAKSDLKELHKVARKSVKKNKKSRIKSEAKEAKLAAKQ
                     AKRETKAAIKEQKRQNKLAKKQAKYELKEKKRQLKSERVELKHELKKSRLMDKVRSFT
                     PKEQNSDSTSSAESIDNNIIDNNITDNNIIDIERKIN"
     gene            complement(4787..5305)
                     /locus_tag="DHV81_08570"
     CDS             complement(4787..5305)
                     /locus_tag="DHV81_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001088352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HCI94488.1"
                     /translation="MRTYENKDELKKEINKSFAKYILEFNDIPESLKDKRFDEVDRTP
                     AENLAYQVGWTTLVLKWEADERKGLHVKTPSDDFKWNQLGELYQWFTDTYAYLSLQEL
                     KDMLNDNINSIYAMIDSLSEEELFKPHMRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIAL"
     gene            complement(5346..6143)
                     /locus_tag="DHV81_08575"
     CDS             complement(5346..6143)
                     /locus_tag="DHV81_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94489.1"
                     /translation="MRLFDKRTPLQKEWDKLEVQEQRFLRKRLEKKESILNQKLEEKI
                     PPKLQGTLDAAFAKAFALIFEKGTGVIEKTYQREKLEQDYQVRQYTADVKQDSKSLRA
                     FSKKARDTGTKNLLLSGASGIGMGVLGIGLPDIPVFTGMILKNIYETALHYGYSYESR
                     EEKYFILLLIQGAVSYGETFCEIDGKLNEFISCGTLPEEYQEKKQIGETAGALSKELL
                     YMKFLQGIPVVGAVGGAYDAIYMKRITEYAGLKYRHRYLDAQKSGMK"
     gene            complement(6362..6742)
                     /locus_tag="DHV81_08580"
     CDS             complement(6362..6742)
                     /locus_tag="DHV81_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94490.1"
                     /translation="MKKISLVVFPALTIVLEALPLGAVCTFAPSPTERVRETFSYFSL
                     IPFGYANFTPLITAILTVVILLLSLISLKKDSVFNALFVLSIITAIISLMPLMYGLNN
                     YSLVGALITIALVTESIFVKAQQK"
     gene            complement(6782..7195)
                     /locus_tag="DHV81_08585"
     CDS             complement(6782..7195)
                     /locus_tag="DHV81_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94491.1"
                     /translation="MKKIGIVLLILISLTGTLLGCGKQDVNKDLESIDASEIKQIELT
                     GTTGGKDGNYSYFFSDKEVVEFIDLLNQVKLGDIVDENKALSSGAVSYYTIYFATDET
                     LTISPGKYFEVEDTYYEFDNYDELWDEFIVFNSIK"
     gene            complement(7271..7600)
                     /locus_tag="DHV81_08590"
     CDS             complement(7271..7600)
                     /locus_tag="DHV81_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCI94492.1"
                     /translation="MKLINTRKFTWIICIIGCLLALVSIFFLPSIIPVHFANGIADDY
                     GRKIQIFLFPILQVLITFLTGREKVKYFLTHSKTFLTDIQFNWMIDGVLLLVMFAEIW
                     IIYASFA"
BASE COUNT         2023 a         1929 c         1399 g         2534 t
ORIGIN      
        1 cctgagcttg ataagcaggg cttgcaggag cttcttgcta tcgggcctgc acacacgagt
       61 gggctttccc tgtttaagga tttgtttgag gttttgccgg gacacttcat gatttactca
      121 caggatgggc tgcatgatga aacctactgg gagcttacaa gccgggaaca caccgagagc
      181 tatcgggata ccgttgctca cacacgtttt ctggtggagg atgctataaa aaggcagatg
      241 gtttccgatg tgccggtatg tacttttctg tcaggcggca tcgattcaag catcgtgacc
      301 gccatcgccg caaactatat gaacacacgc ggtaaaacgc tcaacacttt ttccttcgac
      361 tttaaggaca atgacaggta ttttaaggca aacgccttcc agccggagag ggatctgcca
      421 tatgtcaatc gtatgcttga ggagtatgag acagcccact cctatctcga gtgtgatgag
      481 aactctctct tcaaggcgct ctttgccgca gtcgatgcaa aggatatgcc cggcatgacc
      541 gatgtggact cgtcactttt atatttctgc agcctcgtca gccgaaaaaa caaggtagct
      601 cttacaggtg agtgtgctga tgaaatcttt ggtggatatc cgtggtttta ccgcaaagag
      661 ctgctggaaa gatatggctt cccatggtca tcggatgtcg ctccacgcct tgctcttttg
      721 cgcgatgatg tgggttttga gcttgattta tccggttatc aggcttttcg atatgaggag
      781 tccaggtcaa aagcacctct cctttacggc gaggccggag aggacgaaag ccgccgcata
      841 ataggttatc tcaatatcaa atggttcatg cagacgcttc ttgaccgcat ggatcgcacc
      901 agcatgtact ccgagcttga ggcccgtgta ccttttgccg accatcgtat cattgagtat
      961 gtctttaatg tgccgtggca tatgaaatat caaaacggtg tggaaaagac tctgcttcgc
     1021 gatgcttttt cagatgttct gccacctgag cttttgcaca gaaaaaagag cccatatcca
     1081 aagacttatc atcccggata tgaagctctc cttataaagg gaatggagga gatattggac
     1141 tcccctaacg cccctgtgcg tgctctcatc gacacaggca aaacacgtga attcttaaaa
     1201 gctcccgcag aatacggcag gccgtggttt ggtcagctca tggcaggccc acagcttatt
     1261 gcatatttca tccagattaa ttactggatg gagaaatatc agctgagtgt ttagaaacgc
     1321 agcaccataa agctctgtgc caaaaccgtg acatttccga taatctgcac atttgtctcg
     1381 cgcacatcgg taatctccgg cattccctca gggatttctc ctgttccctc gagtatccat
     1441 gcatgaccta ttgcctcgac cgggacatct gcaccatctg taagtggcag cgtatagtcg
     1501 ctgtcattta catttgcgac cttgagtatc gtctcgccct gctgtgtgcg tgaaagcgat
     1561 acatatatgc cgtccttgcg cagttccttc tcctgaccgt tcataggaat ggtctcatca
     1621 cccacatggt ttgcaaaaag catctgaaca taatagttcg gtgtcagata taccttttca
     1681 tcatcgaacc atatcatatc cggcttccac tggctgtgtc cgacacggtt gaaaagaggc
     1741 gcatatgatg ccagtttaac aacatctgca tttttctcta ttcccgtaag cagggctgcc
     1801 tctgccaatg cactctccat attgttctgc ctgtctgtgg tgtgtgccgc atactcaccg
     1861 gcaaaaacag cgatatccct cggataatca tcatagtgat ccacatgatc atacatccac
     1921 tgtggactta cataatagtg ctcatccacg gcataacagg tgtttggatt agccttgaac
     1981 tcgctgttgt agaaattcca tgcatcctca gtaataggcg aatcaaggaa cggtccggct
     2041 gttccgagta tcctcatatc cggatatttc tcgtggatag ctttctcaaa tatctgatga
     2101 cgcttgtaga gatccacata ctgtgactcc cactgctcgt ttcctactga tatcatatcc
     2161 agcccgaagc tctcaggatg tcccatcttt gcgcggagtg cgccccactt actgtttacc
     2221 ggtccgttgg caaactcgat cagatcaagt gcatcctgca catactcatc aaattcctcg
     2281 ctgtcagatg tcactaactg tgtagacctg aactgacacg ctgtaccgat gcttagtacc
     2341 ggaagcgctt ttgccccgag caactcacaa agcaggaaat actcgtaaaa gcctattcca
     2401 aaggactgtc catagtgtga atccggacgt tttacatccc atccagtctt tgatctgtca
     2461 tcatcaaatg cccatagatt aggaatatac tttctgtctt ttaactcacc tacagttctc
     2521 ttccagcggt atctgttctc aaggcttatg ccctccacaa tacagccgcc cggaaacctt
     2581 ataaatcccg gctttatggc tgccagtgcc tcatacagat cgcgtctgaa aattcctttt
     2641 acggcatcct caggtatcat agatattagg tcaaactcga tgactccttc cccatccagt
     2701 gctattgtaa acttagcccc tgcaacatca tcatcggctg tcagtaccgc atcatatttg
     2761 atccagtcat tgcaacggca cattttgtct gtgtccagct ttttcagctt ctccagctgt
     2821 gcacacataa caggatcttt ttgctccatc tcgatcttta aatctgaaaa tggtacatac
     2881 ggtatggctt ttacagcctc aacagttgtt tctgcgtatg tttttccgtc cttattgact
     2941 gatatcacaa aatcactgcc tgcataatct acgcatctcg cataaaatga tacattgtat
     3001 ttcatgccct tgtgcagtct gataccgtca tatgctttgt tggaaaatcc ctgtcctgcc
     3061 tctgtggcag taaatctcag atagtgtgga ttggcctctg aaagaggtgt tcccataata
     3121 tactgcatac gcggcttatc ctgtacgtct gctgcatatg gctcccacgc atatccgttg
     3181 tcgtccactg agtagaaatt acccggagtg ccgaagccct cctttgcctc aaatgaacgg
     3241 ttctcaatca tctccgcata caaacctccg tctgccgcaa agttgatgtc ctcaaagaat
     3301 aatccaatca tttccggtgt gattttcaca cctcttttgt ctgtaatctg catagcgatt
     3361 acctcctact ataaatttat aataaaaatg cccagcaatt tatagcctta gttaatctta
     3421 cgctctatat ctatgatatt gttatctgtg atattgttat ctatgatatt gttatctatg
     3481 ctctctgccg aagatgtgct gtcactattc tgctctttcg gtgtaaagct tctcacctta
     3541 tccatgagcc ggctcttttt cagctcatgc ttaagctcaa ctcgctcact ctttagctgt
     3601 cgttttttct ccttcagctc gtattttgcc tgtttctttg cgagcttatt ctgccttttc
     3661 tgttccttta tggcagcctt tgtctctctc tttgcctgct ttgccgcaag ctttgcttct
     3721 tttgcttcac tctttatgcg gctctttttg tttttcttta cactctttct ggccacctta
     3781 tgtaattcct tgaggtctga ctttgcagcc tttatattct ttctgtcctt gcggtatgga
     3841 ttgtgcacaa agtatcccac agtgcctatc atcataagaa gcagcgccag tgcctctccc
     3901 actatcgtga catactccag atactgcgga tacagcttgt agcctatcat ctccgctgct
     3961 atcaggataa gtggaatgaa aaacagtata ttctttcgct tttgggacaa tagcgctccc
     4021 tccgcgccta cacacatgag tatatacagg cacaactgtg aggtcggtcc gtaggcatat
     4081 gccacaatcg cagccattat cacagatatg caaaacacga tgaatgcttc gccgcgaaac
     4141 agggcattct tgtactcttt tatgccctcc agcttgtcta ccgatatgcc atgcagtgaa
     4201 tatacctgat ctgtgatgtc ctccatgcag tcctcatggt atctcttcag ctctttgttg
     4261 ctgtttatat cggcatctat ggagcgtctg cgctctgtaa gcagcttctg ctcctccatg
     4321 atcttctccc aggcctcgtc ctccgcttca cgctgcagct tcacctgctc gtgaatccac
     4381 tcagctcgct ggatgcgctc actctcctgc gggataacca cctgctgcgc gtcaactgtc
     4441 tgctgtggta tttgagtctc ctgcgccatg ctgttatttt cattatctat cattccggtt
     4501 ttctgcctct ttcttccaga cattttcttt tcctcttgca attgttattt actgctatat
     4561 atgtctatta aagcatattt tagcggaatt ttccatagga tatgcgcatg ataatcacaa
     4621 tttttgatac ttcatggaat tctaaaaatg attagtaaat catccccaac ttggggacga
     4681 tttgcatctg taatcctttc taatgtgtca gttcgacaaa ttcaatttaa tatgcataga
     4741 catctccttg tccatttttg atttttacga ttacataatc ttgaagttat agtgctattt
     4801 ttttccattt tctaatctta gttctaaatg ttccgaatgg agcaactgtg ttaacatgta
     4861 tgaacttgta tacttcccag acagctgttt tagttgcttc gtcagcccat tttctcatat
     4921 gtggtttaaa taattcttcc tcacttagcg aatcgatcat tgcataaata gaattaatat
     4981 tatcattcaa catatctttc aactcctgca gtgacagata cgcatatgta tctgtgaacc
     5041 attgatataa ttcacccagc tgattccatt taaaatcatc tgaaggagtt ttgacatgaa
     5101 gaccttttct ttcatctgct tcccatttaa ggacgagcgt tgtccatcca acttggtaag
     5161 caagattttc tgccggagtt cggtcaacct catcaaacct cttatccttt aaagattctg
     5221 gaatatcatt aaattccaaa atatactttg caaagctttt atttatctct tttttaagtt
     5281 cgtctttatt ctcgtatgtt ctcaatagtc tacctctata aattccaact cttcaaagca
     5341 acaatttatt tcattccact tttctgtgca tccaaatacc tgtgccggta ttttagccct
     5401 gcatactccg tgatccgttt catgtaaatc gcatcatatg ccccgccgac tgctccgacc
     5461 acaggaattc cctgtaagaa cttcatataa agcagctctt ttgacaaagc tcctgctgtt
     5521 tccccgatct gcttcttttc ctgatactct tccggcaacg ttccacaact gataaattca
     5581 ttcagtttgc catcaatctc acaaaacgtt tccccatagg ataccgctcc ctgtatcaat
     5641 agcaggataa aatacttttc ttccctgctt tcgtagctat atccataatg cagggcagtc
     5701 tcgtagatat tcttcagtat catccctgta aataccggaa tatccggcag accaattccc
     5761 agtactccca tcccaatccc ggatgcgccg gatagcagta aattctttgt tcctgtatct
     5821 ctcgcttttt tagaaaaggc tcgcagggat ttggaatcct gctttacatc cgccgtatac
     5881 tgtcgtacct gatagtcctg ctccaacttc tcccgttgat acgtcttttc aataactccg
     5941 gttcctttct caaaaatcag agcaaacgct ttggcaaaag cggcatcaag tgttccctga
     6001 agtttaggcg gtatcttctc ctccagcttt tgattcaaga tactttcctt tttttccaac
     6061 cgtttccgaa ggaatctctg ctcctgtact tccagcttat cccactcttt ctgaagcggc
     6121 gtccttttat caaatagtcg cattttaata cacctccccg ttcatctgac gttttacatg
     6181 atccagcaaa cattggtccc tgacaccatt aaatttttat aactaataac catagctaat
     6241 gcacttccaa aagttattcc tgattttacc attttcttga cagtcgttga tttattatct
     6301 tccatttttg ttttctccgc atattcctat tttcttaatt ctgcaagctg gaattttcgc
     6361 attatttctg ttgtgcctta acaaaaatgc tttctgttac aagggctatc gttataaggg
     6421 ctcctacaag ggagtaattg tttaatccat acatcaacgg cataagagaa ataattgctg
     6481 taataatcga aagaacaaac aaggcgttaa aaacgctatc tttcttcaaa gaaatcaacg
     6541 aaagcaacaa gataacaaca gtaagtatgg ctgtaataag tggagtaaaa tttgcatatc
     6601 caaacggtat aaggctaaag tatgaaaatg tttctcttac tctttctgtt ggagatggag
     6661 caaaagtaca aactgctccc agtggtaatg cttctaacac tattgttaaa gccggaaaaa
     6721 ccactaagct gatttttttc atgttgtgta cctccgtcta caaatcagaa ttttccgaaa
     6781 cttactttat gctgttaaat actataaatt catcccacaa ttcatcataa ttatcaaatt
     6841 cataatatgt gtcctcaact tcaaaatatt ttcctggact aattgtcagc gtttcatctg
     6901 ttgcaaaata gattgtgtaa taagaaacag ctccacttga taatgctttg ttttcatcta
     6961 ctatgtcacc aagtttcact tgattcaata aatctataaa ctcaacaact tctttatctg
     7021 aaaaaaaata cgaataatta ccgtctttcc cacctgtagt tcctgtaagt tcgatttgtt
     7081 tgatttccga cgcatcaatg ctctccaagt ccttattcac atcttgtttt ccgcatccta
     7141 acaatgtacc tgtaagtgaa ataagtatga gtaaaactat tccaattttt ttcatttaca
     7201 taaccctcca tttacatttc agtttgacaa cttctagctc gacaagctga tagttgttca
     7261 aataatattt ctatgcaaat gaagcataga taatccatat ttctgcaaac attaccaata
     7321 aaagtacacc atctatcatc caattaaact ggatgtccgt taagaaagtt tttgaatgtg
     7381 tcaggaaata ttttactttt tctcgtcctg tcaaaaatgt gataagcact tgtaatatag
     7441 gaaataagaa tatttggatt tttcttccat aatcatccgc aatcccattt gcaaaatgta
     7501 ctggaataat actcggtaag aaaaatatgc tcacaagagc aagcaaacat cctataatgc
     7561 aaattatcca tgtgaatttt ctggtattga taagcttcat aacaatcctt ctttctgtca
     7621 aaattcaaat ttattttaca tctccaactt ccgattatac aagctgagat ttatctctct
     7681 tttcgcttgt cctgctctaa tgcaataaat ccagcggtaa cagcaagggc aagcataata
     7741 tcatgttcgc ctaatgggtc tgttactatg gaataaatca aaaacaccgc acaaaccagc
     7801 aatactgcca atataatcct acgaacaatt tttctttttt ccattgcata acgctccttt
     7861 acaactttcg attttaatgt ctcta
//
LOCUS       DPUF01000249            3309 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_78045, whole
            genome shotgun sequence.
ACCESSION   DPUF01000249 DPUF01000000
VERSION     DPUF01000249.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_78045"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="DHV81_08595"
     CDS             complement(<1..391)
                     /locus_tag="DHV81_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCI94493.1"
                     /translation="MVLLIDTNIILDVLLSRSDFVKESSIIWKLCETEQAKGYISTLT
                     YANMMYIMRKQMTPEQIEDVFRKLKLIFEFADFSSVVLEMAVNMKWKDFEDAIQSATA
                     EYVHADYIITRNIKDFTQSKVMALTPAE"
     gene            complement(375..617)
                     /locus_tag="DHV81_08600"
     CDS             complement(375..617)
                     /locus_tag="DHV81_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HCI94494.1"
                     /translation="MNTATATATEMQNNFGRYLNLVMSGHEIIVTKNGHEVGRFIPKD
                     AAVSYLTDSLTGILQDNGNLDEIKTESMREKYGFVD"
     gene            complement(839..1426)
                     /locus_tag="DHV81_08605"
     CDS             complement(839..1426)
                     /locus_tag="DHV81_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HCI94495.1"
                     /translation="MIITIARKCGCDADSIGRAVAEKYHLHFMNREAIAAGAKEAGCY
                     DEYEEFYSERPVNSLLYSIVMETEEDNVLHDTPARALTFIDRLLLEDGCEGYVIQGRC
                     ANFAFQGRDDVVSVFLTANDAFCVDTVADTHGASARKAKTVVRETNDNRKTYHKYYTG
                     QEWGQAENYDLCLNVAKLGVDGVIAMIDSFIQNRK"
     gene            complement(1483..2175)
                     /locus_tag="DHV81_08610"
     CDS             complement(1483..2175)
                     /locus_tag="DHV81_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCI94496.1"
                     /translation="MDNLFQTSLFAGVTVSLVAYLLGMLLKKKFKLAILNPLLISIVL
                     TIIVLVVADVDYDTYNKGASYLSWFLTPATVCLAIPLYEQWTLLKKNIAAVALGLTAG
                     VITSLVTVLVLSVLMGLSHEDYVTLLPKSITTAIGMGVSEELGGYVTITVAVIVVTGV
                     LGNILGEFVCKIFRITEPISKGLALGASSHAIGTAKAIEIGEIEGAMSSLAIAVAGIL
                     TVVFASVFANFY"
     gene            complement(2193..2570)
                     /locus_tag="DHV81_08615"
     CDS             complement(2193..2570)
                     /locus_tag="DHV81_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase regulator LrgA"
                     /protein_id="HCI94497.1"
                     /translation="MKLLKQFLIILAISFVGEILKYLLPLPVPASIYGMVILFIGLLT
                     GLIPLNSVRSAGKFMIEIMPVMFIPAGVGLMSSWGNLKPVLVPVIVITVVALLTVMIA
                     TGHVSQLVIRMQKRKRADAGQNK"
     gene            complement(2619..3290)
                     /locus_tag="DHV81_08620"
     CDS             complement(2619..3290)
                     /locus_tag="DHV81_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCI94498.1"
                     /translation="MTEKEKIRIQTSETFLLSAILALSGGFQDAYTYNVRNKVFSNAQ
                     TGNVVLMSQHLMIGEWMKGLSYLFPILSFALGVLVAERIGHRYKKAKRIHWRQIVVAI
                     EIIILFVVGFIPHRYDMIATMLVSFSCSMQVQSFRKVNGYGYASTMCIGNLRSGTESL
                     SIYLRDRNIGALKKTAHYYGIILIFAIGAGIGGVCSLHIGIRAIWASSVLLAIVCTMM
                     VREHR"
BASE COUNT          912 a          823 c          580 g          994 t
ORIGIN      
        1 gttccgcagg tgtaagtgcc ataaccttac tttgcgtaaa gtctttaatg ttgcgcgtaa
       61 taatatagtc tgcatgaaca tattcagcag ttgcactttg tatggcatct tcaaaatctt
      121 tccacttcat atttacagcc atctcaagca caactgaact aaaatcagcg aactcaaaaa
      181 taagttttaa cttgcggaat acatcttcta tctgctctgg agtcatttgt tttctcatga
      241 tatacatcat atttgcataa gttaatgtag atatatatcc ctttgcctgt tcagtttcac
      301 aaagtttcca aattatagat gattccttca caaaatctga gcgacttagt aaaacatcaa
      361 gtataatgtt agtatcaatc aacaaaacca tatttttccc tcatactttc ggtttttatc
      421 tcatctaaat taccattatc ctgaagaatt cctgttagag aatccgtcaa atatgatact
      481 gcggcatcct taggaataaa tcttcccact tcatgaccat tctttgtaac aatgatttca
      541 tgtcctgaca taacaagatt caaatatctc ccaaaattgt tctgcatttc tgttgcagtt
      601 gctgttgctg tattcatatg ccttcctcct atccattttc ttttttagct aattatatta
      661 taacgttaat tagctatttt ttcaagatat atccccgttg taacattatt cttgatattt
      721 tttgctgtgt ttgtaacata attccgtaca tttttccaac gatgaatttt tattttcaaa
      781 aacaaactca aaaagccgaa tccacactcc catggattcg gcttttgtat atacacttct
      841 attttctatt ttgtatgaag ctgtcaatca tcgcaatcac accatctaca ccgagctttg
      901 ccacattcag gcacaggtca tagttttctg cctgccccca ctcctgtccg gtatagtatt
      961 tgtgataggt ctttctgttg tcatttgtct cacgcacaac agtctttgcc tttctcgctg
     1021 aggctccatg tgtatcagcc acagtatcaa cgcagaaagc atcattcgct gtcaggaaaa
     1081 ctgagacaac atcatctctt ccctgaaagg caaagtttgc acaccgtccc tgaatcacat
     1141 atccctcaca gccgtcctca agcagcagcc tgtctataaa tgtaagagcc ctagccggag
     1201 tatcatgcag cacattatcc tcctccgtct ccatcacaat agagtaaagc agggaattaa
     1261 ccggtctctc actgtaaaac tcctcatact catcatagca gcctgcctct ttggcaccgg
     1321 ccgcaatagc ctctctgttc atgaaatgta aatgatattt ctcagcgacc gctctaccga
     1381 tactatctgc atcgcaaccg cactttctag caatagtaat tatcatataa atatagctcc
     1441 tttctgtaga aaaagccaac gcgcaggaag cgctcagaca aatcaataaa agtttgcaaa
     1501 caccgatgca aacaccaccg taagtattcc tgccaccgcg atagcaagag agctcatggc
     1561 gccctctatc tcgccaatct cgatagcctt cgctgttccg atggcatgtg atgaagcacc
     1621 aagcgccagt cccttcgata taggctcagt gattctgaat attttgcaaa caaactctcc
     1681 gagtatattt ccaagcacac ctgtcacgac gataactgcg acagtgatag tcacatatcc
     1741 gccgagctcc tctgatacgc ccataccgat agctgtcgtg atggactttg gaagcagtgt
     1801 gacatagtcc tcgtgtgaaa gccccataag cactgacagc acaagcactg tcacaaggct
     1861 tgttatgacg cctgccgtga gtcctagcgc gactgctgct atatttttct tgagcagtgt
     1921 ccactgctca tagagcggta tcgcaaggca taccgtggcc ggtgtcagaa accagctaag
     1981 gtacgatgcc cccttattgt atgtgtcata atccacatct gccacaacaa gcactatgat
     2041 ggtcagtact attgaaatga gaagcggatt gagtattgca agcttaaact ttttctttaa
     2101 aagcatacct agcagatatg caaccagact tactgtgaca ccggcaaaaa gcgatgtctg
     2161 aaacaaatta tccattatga ttatcctcta tcttatttat tttgtccagc atcagccctc
     2221 ttgcgcttct gcatacggat tacgagctgt gaaacatgtc ctgtagcaat cattacagta
     2281 agaagtgcca caaccgtgat tacaatcacc ggtacaagca ccggcttaag attgccccag
     2341 gatgacataa gccccactcc cgcagggata aacatgacag gcattatctc tatcatgaat
     2401 ttgcctgcac tccttacaga gttaagcggt atcaggcctg tcagcagacc tatgaaaagt
     2461 ataaccatgc catatatgct cgccggaaca ggcaatggca gtaaatattt gagtatctca
     2521 cctacaaacg aaatcgcaag tatgatgaga aattgtttta ataatttcat tttatattgt
     2581 atctccgtta tatatatttc gtgactattt gtaactgttt atctgtgttc cctgaccatc
     2641 attgtacata caatggcaag cagcacgctt gaagcccata tagcgcgtat tcctatgtgc
     2701 agtgagcaca ctccgcctat acctgctcct attgcaaata tcagaataat tccatagtag
     2761 tgcgcggtct ttttcagtgc cccgatattt ctgtcacgca gatatattga cagactctct
     2821 gtgccgcttc ttaagttgcc gatgcacatg gtgctggcat agccgtagcc gtttaccttt
     2881 ctgaaggact gcacctgcat agaacagctg aatgatacaa gcatggtcgc tatcatgtcg
     2941 tacctgtgtg gtatgaagcc caccacaaac agtatgatta tctctatcgc caccactatc
     3001 tgacgccagt ggatgcgctt tgccttctta tatctatgcc cgattctctc tgccacaagc
     3061 acaccgagtg caaatgaaag gattgggaaa agatacgata gtcccttcat ccattcacct
     3121 atcatcagat gctggctcat cagcaccacg tttcctgtct gcgcattcga gaacaccttg
     3181 tttctcacgt tgtaggtgta ggcatcctga aagcctccgg aaagtgccag aatcgcactc
     3241 agcaaaaagg tctctgatgt ctgaatcctt attttttctt tttcagtcat tttttcgcct
     3301 ctttttctc
//
LOCUS       DPUF01000250            9542 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_10352, whole
            genome shotgun sequence.
ACCESSION   DPUF01000250 DPUF01000000
VERSION     DPUF01000250.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9542)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_10352"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="DHV81_08625"
     CDS             <1..804
                     /locus_tag="DHV81_08625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94499.1"
                     /translation="NWKTPSVSFKLDKETEVTLVVGIVSAKGGWGTADDLYLYQTKSA
                     DKSDADNGNTGDDDNNKPNPPSADDENKDNTGNGDTATQPAKALTPGAVVASISDTKT
                     TVKDTKGILPDTVKFESKKVTDEKKVAKVADVVKDKIVGVKDVLIYELNLTDGATQLH
                     QLADKVQVTMDMPFALADNEAIKVFRVDSDKLIACTSRVADGKLVFETDHFSTFAFVK
                     VNTNAGTKAAAAGAAKTSDDSNMYMWLVIAMAGLGVSIAALMNRKKEQA"
     gene            complement(932..2383)
                     /locus_tag="DHV81_08630"
     CDS             complement(932..2383)
                     /locus_tag="DHV81_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HCI94500.1"
                     /translation="MKTSEIIVFIVYLLCMLGIGIFFFVKNRNGGEKTYFLGGREMGP
                     WVAALSAGASDMSAWVLMGLPTSIYALGLGQVWIPVGLAIGYTISWLLEAPRLRKFSI
                     VANDSITIPQYLTNRFLSKSLALQVICAIVFLVAYTIYAASSVKACGTLFHTVIGMDP
                     QIAMYLAAVVIVGYTFLGGFSAVCWTDFFQGMLVLGSMFIAPIFAAFMLDTSSMSALP
                     GGYLNPFSSWQDIVSGLAWGLGYFGMPHIIIRFMSIKSQKDLKKSAKIGITWTLLIVF
                     FATLIGVIGRLFLGFDETINENSLVFIQIVRKIFPGVISGILLSAVLAASMSTADSQL
                     LAASSAFSSDVYKPVIRKGRASDKEMMWTGRIVVLGISVAALIIASNPNAGSIMALVS
                     NAWGIFGAAFGPAIMLSLFWKRFNFKGAVAGIITGAAVDILWYAFLTSTGIYELLPGF
                     VLGLIAAVVVTLCTKEPSKEVYELFDKAEKFDE"
     gene            2562..4161
                     /locus_tag="DHV81_08635"
                     /pseudo
     CDS             2562..4161
                     /locus_tag="DHV81_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350753.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    3161..3252
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4276..4319
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4478..5413
                     /locus_tag="DHV81_08640"
     CDS             4478..5413
                     /locus_tag="DHV81_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HCI94501.1"
                     /translation="MIFVAILLLVAGFVALVKGADVFVDGAAALAGKFGIPQLVIGLT
                     IVAMGTSLPEAAVSITAGIKGNAGITVGNIVGSNILNILIILGVTALITNVAIQKTTF
                     KYEIPYMLLITAVLLVMGMTGNEITFIEGVILWVLFIAYLLYLYFLAKKGNDSGDEQP
                     VKLYPVWKCLLFIVLGGVCVVIGSQIVVNGATTIARACGMSERFIGLTIVAFGTSLPE
                     LVTSVSAARKGNAGIAIGNIVGSNIFNILFVIGTVALICPVPFESRFVIDTVVAILCG
                     VLLWGGTIRHKELRKPCGVVMLLCYAAYFVYLAAM"
     assembly_gap    5418..5485
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5703..7495)
                     /locus_tag="DHV81_08645"
                     /pseudo
     CDS             complement(5703..7495)
                     /locus_tag="DHV81_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568159.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase"
     gene            7615..8949
                     /locus_tag="DHV81_08650"
     CDS             7615..8949
                     /locus_tag="DHV81_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI94502.1"
                     /translation="MSKKKKIIISVIAVVLIIAVIVSAVVIAMTKGKNAKKSSGVYAN
                     KVSDVMYQAGSTDRYSGVVEAADSMDIKADSEKKVKDILVSEGDEVQVGTPLFTYDTK
                     DLAAKKESATLELESLNNTLFGYDAQIEQLNKEKKEASKDQQLDYTLQIQQVQTQQKQ
                     TQYDVATKKAEIEKYDESIKNATVNSTMVGVVKKINENQSADTADSAFMTIVATGDYR
                     VKGTIDEQSFLNSGLAEGQAVIVRSRVDESKTWQGTISKVDTENPQTDNSNSGMYDDG
                     NSEQKASKYNFYVQLTGTGDMLLGQHVYVEPDFGQTQMKEGIWIDESYVVKDGDDAYV
                     WTLNKKDKLEKTKVELGDYDENLMMYEIKSGLSKDDYIVWASDDLYEGEPAQTWEEYI
                     ENGGGEDTVYDNMGTEALDDQMLEGTEGGPVDDGTMDEGAADATGSGDAEVQ"
     gene            8950..>9542
                     /locus_tag="DHV81_08655"
     CDS             8950..>9542
                     /locus_tag="DHV81_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HCI94503.1"
                     /translation="MILELQHIYKNYDQDKLVVPVLKDVSLQIEEGEYVAIMGPSGSG
                     KTTLMNIVGCLDLPTSGTYILDGRQVSELKEKELTRVRRDTIGFVFQNFQLMPRESAL
                     DNVCLPLIYAGVSKKDRRQMGMEALERVGLSDRADFKPTQLSGGQKQRVAIARAMVNH
                     PKLLLADEPTGALDSKSGRQVLELFESLNESGVTVVM"
BASE COUNT         2855 a         1740 c         2275 g         2459 t
ORIGIN      
        1 aactggaaga ctccaagcgt ttcgtttaag cttgataagg aaacagaggt tactcttgtt
       61 gtcggaatcg tgagtgcaaa aggtggctgg ggaacagcag atgacctata cttatatcag
      121 acaaagtcag ccgacaagag tgatgccgat aatggtaata ccggtgatga tgacaataat
      181 aagccgaatc ctccatctgc tgatgatgaa aacaaggaca atacaggaaa cggtgatact
      241 gccacacagc ctgcaaaggc tcttacaccg ggagcggttg tagcaagcat atccgacacc
      301 aaaacaactg taaaggacac taagggaatc cttccggata cagtaaaatt cgagtcaaag
      361 aaggtaactg atgagaagaa agtggcaaag gttgcagatg tagtaaagga caaaattgta
      421 ggagtaaagg atgttttaat atatgagctt aatctgacag atggagctac acagcttcat
      481 cagctggcag acaaggttca ggtaactatg gatatgcctt ttgcgttagc tgacaatgag
      541 gcaatcaagg tattccgtgt ggattcagac aagcttatag catgcacatc aagggtagca
      601 gatggaaagc ttgtatttga gacagaccat ttcagcacat ttgcctttgt aaaggtaaat
      661 accaatgcag gtactaaagc agctgctgca ggcgcagcaa agacctcaga tgacagcaat
      721 atgtatatgt ggcttgttat cgcaatggct ggtcttggag tgtcaatagc agctcttatg
      781 aaccgcaaaa aggagcaggc atagcagctc ttctaaaaga acaacataac gcataagaaa
      841 aataaaataa aaaataaaac cgtcgtacca gggccgtaaa tagcagggta cgacggtttt
      901 acttatgtgc tttactgatt aattacttta attattcatc aaacttttcg gccttatcga
      961 agagctcata aacctctttt gaaggctcct ttgtgcaaag tgtcacaaca acggctgcga
     1021 taagtcctaa aacaaatccc ggcagcagct cgtagattcc ggttgatgta aggaacgcat
     1081 accacaatat atcaacggca gcacctgtta tgattcctgc aacagcaccc ttgaagttaa
     1141 agcgcttcca gaagaggctg agcatgattg caggtccaaa ggcagctcca aagatacccc
     1201 atgcattgga aacaagtgcc atgatagagc cggcattagg gtttgatgcg atgatgagtg
     1261 ctgcaacaga gattccaaga acgacaatac ggccggtcca catcatctct ttatcacttg
     1321 ccctgccttt tctgatgaca ggcttgtaca catcacttga gaatgcagat gaagcagcaa
     1381 gcagctggct gtctgcggta ctcatggaag cggcaagcac tgctgagagg agtatacctg
     1441 agataacacc cgggaaaatc tttcttacaa tttggatgaa aacaagagag ttttcattga
     1501 ttgtttcgtc aaagccaagg aaaagtcttc cgattactcc gattaaggtt gcaaaaaata
     1561 caataagcaa tgtccatgtg ataccgattt ttgctgattt cttcaaatcc ttctgtgatt
     1621 tgatggacat gaatctgatg atgatatgtg gcataccaaa gtaaccaagt ccccatgcca
     1681 gtcctgacac gatatcctgc cagcttgaga atggatttaa gtaaccgccc gggagtgctg
     1741 acatagatga tgtatcaagc ataaatgctg caaaaatcgg tgctataaac atagaaccaa
     1801 gcacgagcat gccctgaaag aaatctgtcc agcatacagc cgagaagccg ccgaggaagg
     1861 tatagcctac gatgaccact gcagcaagat acatggcaat ctgtgggtcc attcctatta
     1921 cagtgtggaa aagagtaccg catgccttta cgcttgatgc cgcataaata gtgtatgcaa
     1981 ccaaaaatac aatcgcacat attacctgca gcgcaagtga tttggacagg aatctgttgg
     2041 tcagatactg cggaatagtg attgagtcgt ttgctacaat cgagaatttt ctcagtctcg
     2101 gtgcttcaag cagccagctt atagtgtagc cgattgcaag tcctacagga atccatacct
     2161 gacctaagcc cagtgcatag atagaagtag gaagtcccat gagtacccat gcactcatgt
     2221 ctgaggctcc ggctgacagc gccgcaaccc atggtcccat ctcacgtcca ccaaggaagt
     2281 aggttttttc tccgccgttt ctgtttttca cgaagaagaa aataccgata cccagcatgc
     2341 acagcagata gacgataaat acaataattt ctgatgtttt catttgtagt acctctttaa
     2401 tagtttattt agtaaccatg ctaccacgtt agcgtgataa aggcaagaaa aaattaatga
     2461 taaaacaaaa cgattcattt attatttgta attatataga taatcagcta tactataaaa
     2521 aaatatagct gatttttata tgtcaaaggg gtgaaaacgg gatgggaata tactttaatc
     2581 ctggaaatga gagtttttta cagtcaaaaa acagtagaat atatgtggat aagacagaac
     2641 tgatcaatga attaaatagc agattgtcga cagatgataa atgtattgca gtaagccatg
     2701 caaggagatt tggaaaatcc catgctgccg gaatgataga tgcatattat agcaggggct
     2761 gtgattcagc agagttgttt gaagacacta agcttgcaac aaatccggca tataaaaaac
     2821 atatgaacaa atacaatgtc atacatcttg atatttcctc attttgggat gattataagg
     2881 caaatatcat tgaaaaaatc aaggaatacg ttgtatcgga tttaagggat gaatttgagg
     2941 caaatgttga tttctcaaag aaattcagtg ttatactcat gtctgtttat aagcttaccg
     3001 ggatacagtt tgtaattatt atagatgaat gggattgtgt aatcagaaat agtgatgacc
     3061 agaatctgat tcataattat ttacagtttc ttcattcatt atttaagtca gaggaatcaa
     3121 agtacttcct tgctttggca tatataaccg ggatacttcc nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnattacaca atattatggt tttacagaag aagaagtcat gatgctgtgc
     3301 aggcaataca acatggatta tgagagcatg aaagcctggt acaatggcta tctgatagat
     3361 ggtttgcata tgtataatcc gaattctgtg tcgatggcac ttaattacag ggattttgat
     3421 tcttattgga aaaacacatc atcatttgag tcaatcaata catttataaa catgaattat
     3481 gagggattga gggatgatat tgtaaatatg ctgtcaggga aaaaattata tgtaaacacc
     3541 gaaacttttc agaatgattt ttctactgtt tcctcaaagg atgaagcttt gacagctctg
     3601 attcatttgg gatatctggg atatgacaga gaggaagaga gtgcatttat tccaaattat
     3661 gaagtgaaaa aggcatatca ggcagctctg tgcacaggaa attgggggaa aattggaaaa
     3721 tcaattgcca gatgcgatga attgttaagg gctacaatta attgtgacag tgataaagtg
     3781 gcagaaataa ttgagcttgc acatgaatca tatacatctg ttttgagata taatgatgaa
     3841 aactcattaa gctgcgttct gacaatggct tattttacag cacctgcata ttacaatatt
     3901 gtcagagaaa tgccggcggg taaaggatat gcagatatgg tatttatacc aagagaaaat
     3961 gccggaaaac gcccggcaat tataattgaa ttaaaatgtg ataagactgc cgaggctgca
     4021 gttaaacaga ttaaggacag gcgctatgag ggagcactgg caggttataa gggaaagctt
     4081 cttttggttg gaatcaatta tgatagtcaa aatggtgaaa aaaagcatca tacctgcgtc
     4141 atagaggaat atgaaaaata agcacatttc cctattgacg aataccctgc gttgacatat
     4201 catagtatat aagtgaaagg gagtagccgg cacgagagtg cttacttatc cgtcagtacg
     4261 attgctcata catatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     4321 cagcaatccg gagagtgatt aaatggcgag acttttgttg ttttatacag cagaagtgtc
     4381 gccttttttg tatataaaag ccttctgctg ataggacata gctgtaacta tcaattaatc
     4441 aggcatcctt aaaaaggacg gcagagggag gaaaattatg atttttgtag caattttact
     4501 gcttgtagca ggctttgtag cactggtgaa gggcgcggat gtatttgtgg atggagctgc
     4561 ggcgctcgcc gggaaatttg gtattccgca gcttgtgatt ggactgacga ttgtggcgat
     4621 ggggacgtcg ctgccggagg cggcagtcag catcactgca ggaataaagg gtaatgcggg
     4681 tatcactgta ggtaatatag tcggcagcaa tatactaaat atccttataa tcctgggagt
     4741 gacggcactc atcacaaatg tagccataca aaaaactacc ttcaaatacg agataccgta
     4801 tatgcttctt atcacagcag tgcttctcgt gatgggaatg acaggtaatg aaattacatt
     4861 cattgaggga gttatactgt gggttttatt tattgcatat ctgctttacc tgtattttct
     4921 ggcaaaaaag ggcaatgata gtggcgatga gcagccagta aagctatatc cggtatggaa
     4981 atgcctgctc tttattgtgc ttggcggtgt gtgcgtcgta attggaagcc agattgtcgt
     5041 aaacggtgcc acaactatag cgagagcctg cggcatgagt gagcgcttta tcggactgac
     5101 aatcgtggca ttcggtacat cactgccgga gcttgtgaca tctgtttcag cagcacgcaa
     5161 gggaaatgca ggaattgcaa taggaaatat agtcggcagc aatattttta atatactctt
     5221 tgtaattggt acagtagctc tcatttgtcc ggtaccgttt gagagcaggt ttgtgattga
     5281 tacagttgtg gcaatactgt gcggtgtgct cctgtggggc ggtacgataa gacataaaga
     5341 gcttagaaag ccatgcggag tggtgatgct gctttgctat gcggcatatt ttgtatatct
     5401 ggcagctatg taatgatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnntttaa catcaaaatg attgtgattt cacgctaaat
     5521 ataaacaatt aattaaaaat agcactccaa cttgacaagt gctgattgca ggtgtataac
     5581 gtagtcagaa cgaacggaag gaagcgatta taatggataa aaacccaaag catccattga
     5641 agaaaaagaa taaggcagat gcaaagaagc atcagaagca cgtatacgaa tagcagcatc
     5701 cgttaaggaa tactatccag taagcacaat gtaccccagc ccgtcacagg gttggggtat
     5761 tttgtgtttt tggaagtatt ttcagagaca gcccttgaga aagctgtgaa tacattttca
     5821 cctgcacctt ttatgagaga agctttaagc ttgtcgccat acatatagag gtcattttca
     5881 cgcacaatgc cccattccat caggagcgct gccgcaccgg ttacgaacgg agcagccatg
     5941 gaggtgcctg tttttgaagt gtagcctccg gtgtttgaac aggagataat gtccacaccg
     6001 ggcgctatga tatcaggctt tatgctgtca aaggcccagg tatagcctcg gccggaaaag
     6061 gcagctgtgc ggtttgtgcg gctgtcatag gctccgacag agatgacatt ttgtgaggtc
     6121 gaggggatgg tcagggtgat atcgggtgat gcattgataa atgaggtctg tgcattgcgc
     6181 agagcacctg acggcagcca tatatcccat gaaccgtcct ttacagatat gggcataagc
     6241 gatattttcc agatgccggg tgagatgtat tctgctccca tgagttcaat gaatatttcc
     6301 tgaaagggag agtagggtga gggaccgccg cctgttatat atatgtctgt cccatcgagc
     6361 cggtatctgt atgtaaagcc ctttgcctga ttggtaattg tatctggaat ctccagcata
     6421 tcattatttg gggcggtaat ttgcagttgt atctcatccc agtatttctt ccataaatct
     6481 atggaaagag atggctcagg atagcctatc gcaagctctg catcggcagg gtatgatttg
     6541 ccgtttgcat gaccaaagcc ggctccatcg ttacctgagc cgacagagac cgcgcagcgg
     6601 taattttctg ctatatagtc caggtatgtt tcaagcagtg aggtaccgct gtgcgagccg
     6661 ttacttgtgc caaagcttag gtttatggct ataggcatat tcatatcgat ggattttctt
     6721 atgcaaaagt ctactgccag cataatctgn nnnnnnnnaa gctggcaccg cctgaggttt
     6781 taagctttac gactataaga gtgctctcgt aagcaacgcc acgatatttc gtattttgtt
     6841 cattaccatt tccgtttccg gcagcgattc ctgccacatg agtgccgtga cctgataggt
     6901 cgatgcttgg acagatgcgc tgcatttcta agctgttttc tgcatttatg gctttggtga
     6961 ttgtatcgcg gtcatataag gtgccaaaag gaactatgtc actgtgtgag gagttatcag
     7021 atacagtctg gtcccacaga taggcaattc tgcttacacc atcctgtata aaagccggat
     7081 gggcatagta aattccggag tcgataatgc ctacgagcac accttttccg gtcagattat
     7141 atggattggt gtgtacggga gtgatgcatg aggcctgcga gccctcgtat acatcgtagt
     7201 taaactgctt tggcttttcc atatatatga tttcatcgca cagagcgacg gtatttacaa
     7261 gtgatttttc aattacaagc accgcatagt ttccaagcag ctttgtgatg agtacagaag
     7321 gatatttttc ttttattttt tcgatgtcac cgacatattt tactatcact tcccagctgt
     7381 cactctccgg cagatagccg cttgaaagcc ctccctcttt accaagctca taaggggtgg
     7441 acagcagggc ggcggacagc tgtggagaaa ttttctcact aaatgctatt tccatactaa
     7501 cctccatttt ttattagtaa aatattaaaa aaatcttaaa agacagatgc aatatatatt
     7561 tggtatgatt aattcataat caatcaatta attataagaa accgggaggt cgaaatgagt
     7621 aaaaagaaaa agattatcat ttcagtgatt gcagttgtgc tgattatagc agttattgtg
     7681 tcagctgttg tcattgcaat gacaaaggga aagaatgcaa aaaagtcatc gggggtgtat
     7741 gcaaacaagg tctctgatgt catgtatcag gcgggaagca ctgacaggta cagcggtgtc
     7801 gtggaagcag ccgatagcat ggatatcaag gctgatagtg agaaaaaagt aaaggatatt
     7861 ctcgtgtcag agggggatga ggtacaggtg ggtacacctc tttttaccta tgatacaaag
     7921 gatcttgctg cgaaaaagga aagtgccacg cttgagcttg agagcttaaa caatacgctt
     7981 ttcggctatg atgcccagat agagcagctc aacaaggaaa agaaggaagc gtcaaaggat
     8041 cagcagcttg actatacact gcagattcag caggtgcaga cacagcagaa gcagacacag
     8101 tacgatgttg ccacgaagaa ggcagagata gaaaagtacg atgagagcat caaaaatgcc
     8161 actgtaaata gcacaatggt cggtgttgtt aagaaaatca atgagaatca gagtgcagac
     8221 acagcagatt ctgcatttat gacaattgtt gccaccggcg attacagggt aaagggcaca
     8281 atcgatgagc agagcttttt aaacagcgga ctggcagagg ggcaggcagt tatcgtgcgc
     8341 tcaagagttg atgagtcaaa gacctggcag ggtaccatca gcaaggttga cactgagaat
     8401 cctcagactg ataacagcaa ttcaggcatg tatgatgatg gaaacagcga gcagaaggcc
     8461 tcaaagtaca acttctatgt gcagcttaca ggcacaggcg atatgctttt aggacagcat
     8521 gtatatgtgg agccggattt tggacagaca cagatgaagg aaggcatatg gattgatgag
     8581 agctacgttg taaaggatgg cgatgatgca tatgtctgga cacttaataa aaaggataag
     8641 cttgagaaga caaaggttga gcttggagac tatgacgaga atctcatgat gtatgaaatc
     8701 aaatcagggc tttcaaagga tgactacata gtgtgggcat cggatgacct ttatgagggt
     8761 gagccggcac agacgtggga agagtacatt gaaaatggcg gcggtgaaga cacagtttat
     8821 gataatatgg gtacagaagc tcttgatgac cagatgcttg agggcactga gggcggtccg
     8881 gttgatgatg gcacaatgga cgaaggtgct gcagatgcca ccggttcagg tgacgcggag
     8941 gtacagtaga tgattctaga actccagcat atttacaaaa actacgacca ggataagctt
     9001 gtggtgccgg ttttaaagga tgtttcactc cagattgagg agggagagta cgtggcaatc
     9061 atgggaccgt cgggctctgg caagaccact ctcatgaata ttgtgggatg tcttgatctg
     9121 ccaacatcgg gcacatatat acttgacggc agacaagtca gtgagctcaa ggaaaaggag
     9181 cttacgagag tcagacggga tacaatagga tttgtattcc agaatttcca gctcatgccg
     9241 cgtgagagcg cacttgacaa tgtatgcctg ccactcatat atgccggtgt gagcaaaaag
     9301 gacagaaggc agatgggtat ggaggcattg gagcgcgtag ggctttcaga cagggctgat
     9361 tttaagccaa cacagctttc aggaggacag aagcagcgag tggcgatagc gagagctatg
     9421 gtaaatcatc caaagcttct gcttgcggat gagcctacgg gagcacttga ctcaaagtca
     9481 ggcaggcagg tgcttgagct ctttgagagc cttaatgagt ccggtgtgac ggttgtcatg
     9541 at
//
LOCUS       DPUF01000251           15131 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3362, whole
            genome shotgun sequence.
ACCESSION   DPUF01000251 DPUF01000000
VERSION     DPUF01000251.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 15131)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15131)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15131
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3362"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="DHV81_08660"
     CDS             <1..1005
                     /locus_tag="DHV81_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515700.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCI94504.1"
                     /translation="QWVTLPLALHGVICKKDGSTVTVNIGDKPNDPVVGVSDLLIHLS
                     GEQMGKKASEVIKGESLDVLIGSIPGPEKDNDDKDIKERVKANILTILSQEYGVDEDD
                     FLSAEIEVVPAGEARDYGLDRSMIMGYGHDDRVCAYPSYRAMLEIEGAPEYTSVCLLV
                     DKEEIGSVGASGMQSRFFENCVAEVMNLAGDYSELAVRRALKNSKVLSSDVSAAFDPN
                     YPSVMEKKNSAYFGKGLVFNKYTGARGKSGSNDANAEYVARLRNIMDTADVSFQTAEL
                     GKVDEGGGGTIAYILANYDMNVIDSGVPVLNMHAPWEIISKVDLYEAFRGYIAFLKEA
                     "
     gene            1022..1981
                     /locus_tag="DHV81_08665"
     CDS             1022..1981
                     /locus_tag="DHV81_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCI94505.1"
                     /translation="MNQTFVKSVTEQLPQLGLLQDEPMKKHTTFRIGGSADYYAEPDM
                     SQISKLIEMAKACDMPVTVIGNGSNLLVGDKGIRGLVIGIGKGLSEIDVTEAVAQQST
                     AQDLTAQDNGHIITAGAGAILAAVAAKAAEASLSGLEFASGIPGSVGGAVVMNAGAYG
                     GEIKDVLIDATVLTAEGELKTVTRDELDLSYRHSIVQEKGYIVLSARFRLTPKPKDEI
                     KSYMAELRTKRVEKQPLEYPSAGSTFKRPEGYFAGKLIMDAGLRGYSVGDAQVSQKHC
                     GFVVNKGEATAADVLTLIKDVQETVLKQFGVKLEPEVKMIGEF"
     gene            1981..2856
                     /locus_tag="DHV81_08670"
     CDS             1981..2856
                     /locus_tag="DHV81_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007889367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCI94506.1"
                     /translation="MKFVIVTGMSGAGKSTAMKMMEDMGYFCIDNLPIQLLDKLIDFS
                     NTFHSDVSKVAVGIDVRNGSGIDAIPQTLEQLRQKNFPYEILFLDAEDEVLVKRYKET
                     RRNHPLAGSERINKGIVLEREKLQYLKDNADYIIDTSQLLTRELKIELEKIFVQNEDY
                     KNLFITILSFGFKYGIPSDSDIVMDVRFLPNPYYVDGLRAKTGNDKEIQDYVMQFPEA
                     NEFIDKLDDMIKFLIPNYISEGKNQLVISIGCTGGKHRSVTLANELYKRLSGCNDYGL
                     KIEHRDIGKDALRGK"
     gene            2858..3727
                     /gene="whiA"
                     /locus_tag="DHV81_08675"
     CDS             2858..3727
                     /gene="whiA"
                     /locus_tag="DHV81_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCI94507.1"
                     /translation="MSFSSDVKEELAKQVSKSRHCQLAELAGIIELSGRINEKTKGLE
                     LDTENLLLLNKYATLMKRAFGTDTTKALDEQDTGKILAALKITAQPVNRQTADGLLLM
                     QTCCKRAFIRGAFMAAGSISDPNKAYHFEIVCHTKEQAKQLQELLCGFDTDAKIVERK
                     GHYVVYIKEGAHIVDSLNIMEAYVSLMKLENVRILKEMRNSVNRKVNCETANISKTVN
                     AAVKQIEDIRLIQKMRGLDDLPAQLREMALLRLEYPDAPLKELGGYLDPPMGKSGVNH
                     RLRKLSAIAEELR"
     gene            3728..4612
                     /locus_tag="DHV81_08680"
     CDS             3728..4612
                     /locus_tag="DHV81_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCI94508.1"
                     /translation="MKKKLLFVFNPKSGKGLIKEHLVNIVDIMTKAGYKITIYPTQCQ
                     GDAIKKVRKNAKKYDLVVCSGGDGTLDEVVTGMEQSEVNVPIGYIPAGSTNDFANSLG
                     IPKNMEEAARVAVNGTPFPCDVGGFNGDTFVYVAAFGLFTEVSYQTSQQMKNILGHAA
                     YILEGAKHLMDITSYRMKVSHDGEIIEDEFIYGMVTNSLSVGGFKGISGPDVLLDDGL
                     FEVTLIKNPKNPIELNKILAGLANRENDNELIYSFKTRELHVESEEEVPWTLDGEFGG
                     SHTDLTITNLNKQITIMC"
     gene            4744..5629
                     /gene="fba"
                     /locus_tag="DHV81_08685"
                     /pseudo
     CDS             4744..5629
                     /gene="fba"
                     /locus_tag="DHV81_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741603.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase, class II"
     assembly_gap    5320..5401
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5947..8070
                     /locus_tag="DHV81_08690"
     CDS             5947..8070
                     /locus_tag="DHV81_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007889383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HCI94509.1"
                     /translation="MSEQEYFNVADIFGENVFNDAVMQERLPKKVYKKLHEVMDEGKE
                     LDLETADVIAHEMKEWAIEKGATHYTHWFQPLTGVTAEKHDSFITSPMPNGKVLMSFS
                     GKELIKGEPDASSFPSGGLRATFEARGYTAWDCTSPAFVRQDAAGATLCIPTAFCSYT
                     GEALDQKTPLLRSMEAINDQALRLLRLFGNTTSKKVTPSVGPEQEYFIVDREKYLQRK
                     DLIFTGRTLFGAMPPKGQEMDDHYFGAIRERIAAYMRDVNKELWKLGVSAKTQHNEVA
                     PAQHELAPIYAECNVAVDHNQLIMETLKKVAGRHGLQCLLHEKPFAGVNGSGKHDNWS
                     ITTDDGINMLEPGKTPHDNVQFLLVLTCMLKAIDEHAALLRAAAADVGNDHRLGANEA
                     PPAILSIYLGDQLGDVLNQLISTGTATHSLKGEKLETGVKTIPDFMKDATDRNRTSPF
                     AFTGNKFEFRMVGSQDSVAQANIVLNTIVAEAFSDACDILEKADNFDLAVHDLIKDYA
                     VEHQRIVFNGNGYSDEWVAEAERRGLPNIKSMVDAIPAYVAPESVAAFEKFGVFTKTE
                     LESRAEIEYETYAKTINIEAKAMIDIAGKQIIPAVIKYITSLAQSVNSVKSAVADADV
                     SVQSELLTESSALLSDAQVALAKLKDETAKAGAMEEGREQAVYYRDVVKTAMDALRTP
                     VDELEMIVDKEMWPMPSYGDLIFEV"
     gene            8258..>9189
                     /locus_tag="DHV81_08695"
     CDS             8258..>9189
                     /locus_tag="DHV81_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HCI94510.1"
                     /translation="MTEEISGVIFGVWFLIGAALVFWMQAGFAMVEAGFTRAKNTGNI
                     LMKNLMDFCIGTVVFILIGFSLLLGEDVLGFIGKPGFDIFTSYKDFDWSNFVFNLVFC
                     ATTATIVSGAMAERTKFISYCVYSGVISALIYPIEAHWIWGGGWLSQIGFHDFAGSCA
                     IHMVGGISALVGAAILGPRIGKFTKDKNGKITKVNAIPGHNITIGALGVFILWLGWYG
                     FNGAAAKSVEQLGSIFVTTTIAPALATVVCMIFTWLKYGKPDVSMCLNASLAGLVAIT
                     AGCDVTDALGAIIIGSVAGLLVCFGVWFLDNVLRV"
     assembly_gap    9190..9326
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9327..10016
                     /locus_tag="DHV81_08700"
     CDS             <9327..10016
                     /locus_tag="DHV81_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HCI94511.1"
                     /translation="FYGGGFKLLGLQLTGFVSVAAWTVVTITIVFLVIKATLGLRVSE
                     EEEIVGLDPTEHGLPSAYAGFAIMDVSGDVMDVNENTDLGSSEYATASQAKKDAAVPV
                     VNATTPASASGIHKVVIISKLTKYDKLRKAMNDLGVTGMTVTQVMGCGIQKGAGEKYR
                     GAELDATLLPKVKVEVIVGKIPVEKVIETAKKTLYTGHIGDGKIFVYDVAQVVKVRTG
                     EEGIEALQDVE"
     gene            complement(10124..12901)
                     /locus_tag="DHV81_08705"
     CDS             complement(10124..12901)
                     /locus_tag="DHV81_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCI94512.1"
                     /translation="MNYGKKATMQLLRKYDNKSSKVKNKFKLIVLKILLVVVIVAGAA
                     GISSGIGVMKGIIDSAPDISKIDVTPTGYSTTVLAANGEETATLVGQGANRQYVTIDE
                     IPLDLQHAFVAIEDERFYDHNGIDLHGIGRAFISGLSKGRFSEGASTITQQLIKNNVL
                     TSWTSETSFVEKLQRKIQEQYLALELEKQVNDKDWILENYMNSVNLGANTLGVQAASK
                     KYFNKDVSELTLSEASVIAGITQNPSGYNPITHPDKNAKRREKVLNNMKDQGYISKAQ
                     YDEAMADDVYSRIAEYNTTGSGSVNTYFIDALIDNVFDDLTAAGYSETEAYKLIYKGG
                     LTIKSTQDLTMQTICDEEANNPSNYPSDAKYSFQLSFEVKKADGSYKTYTNQTMLSFY
                     KKKTNNDDFSINYSSPDECNAAIAQYEQDVLEEGDSIVEGSEAVNITLEPQVAMTVID
                     QSTGEVKALVGGRGDKSGNRTWNRATDTCRQPGSTFKIIGCYAAALDAGGKTLASVQD
                     DAPFTVGSKTFNNYDKSFGGFTSIRWAITKSINIVTVKTLQDIGVDLGYKYAEDFGIS
                     TLTDSDKNLSLALGGLTKGVTNLELTGAYATIANGGVYLEPKFYTQVLDHDGNVLLDK
                     TTTQNTRTVIKDTTAWLLTDAMKDVLTQGTGKLARFDSQIAQAGKSGTTTSNRDCLWA
                     GYTPYYTCVVWGGYDDNSKQSGKLTSYPKNIWRNAMSRIHEGLETKDFVQPDGITNTT
                     VCSKSGLVPLEGTCDNDPRGSMLTTEYFDTDTVPTDSCDHHVALEICADSGAIAGPYC
                     PNKTSKVFITGAVSGSPEYEFMADDTFMNTVCTLHDATSLINSSPTTTDPTNSGTTPG
                     STDGTAAGAQTGGAETGTGASSGAGTGSSGTGTGGSGSSGSGAGASGTGGSGSGGTDT
                     GSSGSSGNTHR"
     gene            13067..13774
                     /locus_tag="DHV81_08710"
     CDS             13067..13774
                     /locus_tag="DHV81_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCI94513.1"
                     /translation="MHECNQAISGWKEYHMSENTISLYVYKGGQGEITEKKSRFIATV
                     RPVESEDEAVSFINETKKKYWDARHNCSAFVIGKRQELTRCSDDGEPAGTAGRPMLDV
                     LLKENIHNVAVVVTRYFGGVLLGTGGLVRAYQQATKAGLSASEIIEKKDGAVLFIRTD
                     YTGIGRLQYLFAQEKITVMDTAYEADVLVKAVIPENDKKRIEKTIIEQTNGTAKLEWG
                     DEVTFAEYDGEVLLFKN"
     assembly_gap    13912..13935
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13936..13990
                     /locus_tag="DHV81_08715"
     tRNA            13936..13990
                     /locus_tag="DHV81_08715"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:13974..13976,aa:Ser,seq:cga)
     gene            14099..14278
                     /locus_tag="DHV81_08720"
     CDS             14099..14278
                     /locus_tag="DHV81_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94514.1"
                     /translation="MNKNFLRIINLIEELGSEKKTPITIQQYQDIINKSSNLWMSNGV
                     DEAFRFIRSYFNFID"
     gene            14290..15015
                     /locus_tag="DHV81_08725"
     CDS             14290..15015
                     /locus_tag="DHV81_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase"
                     /protein_id="HCI94515.1"
                     /translation="MAFSFDSRIRYSEVDSSCRLSLTGLTNYFQDCSVFHSQSHDVGI
                     RFLADNHIAWVLSSWQICINRLPLLNEQVKISTWAYGMKAFYGYRNFTLEDAGGSTLA
                     YANSVWVLVDTRTGRPVKVPQEFADTYGLEPQLEMECAKRKLHIPDDMEKKGEIVVPQ
                     FFIDSNHHMNNEKYVMLAQQLLPNDFEISELRVEYRKEAKLGDTVISYVKYTSKSVTV
                     VLADTDKKPYSVVEFLSKPVPGK"
BASE COUNT         4323 a         2959 c         3744 g         3862 t
ORIGIN      
        1 cagtgggtta cactgccgct tgcacttcac ggagtcatct gcaaaaagga tggcagcaca
       61 gtgacagtca atatcggaga caagccaaat gatcctgtag tgggtgtgag cgatctgctt
      121 attcatctgt caggcgagca gatgggcaag aaggcttcag aggtcataaa gggcgagagc
      181 cttgatgtgc taatcggaag cataccggga ccagaaaagg acaatgatga caaggatatc
      241 aaggagagag taaaggccaa tatcctcaca atactttcac aggagtacgg tgtggacgag
      301 gatgatttcc tttcagcaga gatagaggtg gtacctgccg gagaggcaag agactacgga
      361 cttgacagaa gtatgatcat gggttacggc cacgacgaca gagtgtgcgc atatccttca
      421 tacagagcta tgcttgagat agagggcgca ccggaatata caagtgtatg cctgctcgtg
      481 gacaaagagg aaatcggctc agtgggagcc agcggcatgc agtcacgctt ttttgaaaac
      541 tgtgtggcag aggtgatgaa cctggcagga gactacagcg agcttgcagt cagacgtgca
      601 ctgaaaaact caaaggtact ctcatcagac gtatcagcag cctttgaccc gaattatcca
      661 tcggttatgg aaaagaagaa ctcggcatac tttggaaagg gccttgtatt caacaaatat
      721 acaggcgcca gaggcaaatc aggctcaaac gatgccaacg cagagtatgt tgcaaggctt
      781 agaaatatca tggatacagc agatgtttca ttccagacag cagagcttgg aaaggtagat
      841 gagggaggcg gcggaacaat cgcatatatc ctcgcaaact atgacatgaa tgttattgac
      901 agcggtgtgc cggtgctcaa catgcatgcg ccatgggaaa tcatcagcaa ggtcgattta
      961 tatgaggcat tcagaggcta cattgcattt ttgaaggagg cataggctaa aggaatataa
     1021 gatgaatcag acatttgtaa aaagtgtaac agaacaattg ccgcagcttg gtctcctgca
     1081 ggatgagccg atgaaaaaac acacgacatt ccgcatagga ggctcggcag attactatgc
     1141 agagcctgat atgagtcaaa tttcaaagct tatagagatg gctaaggcat gtgatatgcc
     1201 ggttacagtg ataggaaacg gaagcaatct gctggtagga gataagggca tcagaggcct
     1261 tgtaattggt attggaaaag gcctgtccga aatcgatgtg acagaagctg tggcgcagca
     1321 aagcacagcg caggacctca cagcacaaga caacggtcat atcatcacag ccggcgccgg
     1381 ggctatcctt gcagcagtgg cggcaaaggc agcggaggca tcgctttcag gtcttgagtt
     1441 tgcatcgggc atacccggaa gtgttggagg cgcggtagtc atgaacgcag gagcctacgg
     1501 tggagagata aaggatgtcc taatcgatgc cacagtgctc acagcagagg gcgagctaaa
     1561 gactgtcaca agggatgagc ttgacttatc ctacaggcac agtatagtcc aggaaaaggg
     1621 ctatatagtg ctgtcagcca gattcaggct cacaccaaag cctaaggatg agataaagtc
     1681 atatatggca gagcttcgca caaagagagt ggagaagcag ccgcttgagt acccaagtgc
     1741 aggcagcacc tttaagagac cggagggcta ttttgcaggg aagcttatca tggatgcagg
     1801 tcttcgaggt tacagcgtgg gagatgcaca ggtgtctcag aagcactgcg gctttgtagt
     1861 caataagggc gaagccactg cagcggatgt cctgacactc ataaaagacg tgcaggaaac
     1921 agttttaaag cagtttggag taaagctcga accggaagtt aagatgattg gagaattcta
     1981 gtgaaatttg taatagtgac gggcatgtca ggtgccggaa aaagcacagc aatgaaaatg
     2041 atggaggata tgggatattt ttgtatagac aatctgccta tacagctatt agataagctc
     2101 atagactttt caaatacctt ccacagtgat gtgtccaagg tggcagtcgg catagatgtg
     2161 cgaaacggtt caggaattga tgcgattcca cagacgctcg agcagctaag gcagaaaaat
     2221 tttccgtatg agatactttt cctcgatgct gaggatgagg tgcttgtaaa gcgatacaag
     2281 gagacgaggc gaaaccatcc tctcgcaggc agtgagcgca tcaataaggg catcgtgctt
     2341 gagagggaaa agctgcagta tttaaaggac aatgctgact atatcataga cacaagccag
     2401 cttctcacaa gggagcttaa aattgagctg gaaaagatat ttgtgcagaa tgaggattac
     2461 aaaaatctgt ttatcacgat actgtccttc ggcttcaagt atggcatacc gtcagactct
     2521 gatatagtca tggatgtgag gtttctgcca aatccctact atgtggacgg actacgcgcg
     2581 aaaaccggca atgataagga gatacaggac tatgtcatgc agtttccgga ggcaaatgag
     2641 tttatcgaca agcttgatga tatgataaag tttctcattc caaactatat ctctgagggt
     2701 aaaaaccagc ttgttatatc tataggctgc accggaggca agcaccgctc ggtaacgctt
     2761 gcaaacgagc tgtacaagag gcttagcggc tgtaatgact atggacttaa gatagagcat
     2821 cgcgacatag gaaaggatgc attgagaggt aaataacatg tcattttcca gtgatgtaaa
     2881 ggaagagctg gcaaagcagg tatcaaagag caggcactgt cagctcgctg agcttgcagg
     2941 catcatagag ctttcgggcc gtatcaatga aaaaacaaag ggacttgagc tcgacaccga
     3001 aaacctgctg cttttaaata aatatgccac acttatgaag cgcgcctttg gcacagatac
     3061 cacaaaggcg ctggatgagc aggataccgg aaagatactt gcagcgctta aaatcacggc
     3121 acagcctgtc aacagacaga ccgcagacgg actgcttctt atgcagacat gctgcaagag
     3181 agcctttatc aggggagcct tcatggcagc aggctcgata agcgacccca acaaagcgta
     3241 ccattttgag atagtgtgtc atacgaaaga gcaggcgaag cagcttcagg agctcttgtg
     3301 cggctttgat acggatgcca agatagtaga gcgcaagggg cactatgtag tgtacataaa
     3361 ggagggcgcc cacatcgtgg acagtctcaa catcatggag gcgtatgtgt cactcatgaa
     3421 gctggaaaat gtgcgcatat taaaggaaat gcgcaacagt gtaaaccgta aagtaaactg
     3481 cgaaaccgca aacatcagca agactgtgaa tgcggcagta aagcagatag aggatatcag
     3541 actgatacag aaaatgagag gactcgatga tttaccggcg cagcttcggg agatggcgct
     3601 gcttcggctt gaatatccgg atgcaccgct aaaggagctc ggaggctatc tggatccgcc
     3661 gatgggtaaa tcgggagtca accacaggct tagaaaatta tcggcgattg cggaggaact
     3721 gagatagatg aagaaaaagc ttttatttgt atttaatccc aaatcaggca agggactcat
     3781 aaaggagcac ctggtaaata tcgtggacat catgacaaag gcaggataca aaatcaccat
     3841 ttatcctaca cagtgtcagg gagatgccat aaaaaaggtc aggaaaaatg ctaagaagta
     3901 tgaccttgta gtgtgcagcg gcggagatgg cacactcgac gaggtagtga ccggcatgga
     3961 gcaaagcgag gtaaatgtac caataggata catccctgcg ggcagcacca atgactttgc
     4021 aaactctctc ggcataccaa aaaatatgga ggaggccgca agagttgccg taaacggtac
     4081 tccattcccg tgtgatgtgg gaggcttcaa cggagatacc ttcgtatatg tcgcagcctt
     4141 tggccttttt acagaggtat cctaccagac ctcgcagcag atgaaaaata tactgggaca
     4201 tgcagcatat attttagagg gtgcaaagca cctgatggat atcacctcgt acaggatgaa
     4261 ggtgagccat gacggtgaga taatagagga tgagtttata tatggaatgg tgaccaactc
     4321 cctttcggta ggaggcttca agggcatatc aggtcctgac gtactgcttg atgacggcct
     4381 ttttgaggtg acactcatca agaatccaaa aaacccaatc gagctcaaca agatacttgc
     4441 gggactggca aaccgtgaaa atgacaatga gctcatatac tccttcaaga ccagagagct
     4501 gcatgtggag agtgaggagg aggtaccgtg gacactcgac ggagagtttg gcggcagcca
     4561 cacggatctg accatcacta atctgaacaa acagattacc attatgtgct gataaataca
     4621 aaatcggaca taaaatagta atagtacaca attttttgtc tgattgtgtt aaaaatttaa
     4681 ctttattttt ataaggttat gtaatataat aacattagct tttaattaac aggaggaaaa
     4741 aatatgttag tatctgcaac agaaatgctt aagaaagcaa aagaaggaca ttacgcagta
     4801 ggtcagttca acatcaacaa ccttgagtgg acaaaatcag tacttttaac agcagaggag
     4861 cttaagagcc ctgtaatcct tggtgtatct gagggtgccg gaaagtacat gacaggcttc
     4921 ggtacagtag cagcaatggt taaggctatg catgacgagc tcggtatcac agtacctgta
     4981 gcacttcacc ttgatcacgg cacatacgaa ggatgctaca agtgcatcaa ggccggcttc
     5041 acatcaatca tgttcgacgg atctcattat ccaatcgatg agaacgttgc aaagacaaaa
     5101 gagttagtat cagttgctca tgcaatgggc atgtctatcg aggctgaagt tggttcaatc
     5161 ggtggcgagg aagacggagt tgttggaatg ggcgagtgtg ctgatccaga cgagtgcaag
     5221 agagttgctg atcttggcgt agatatgctt gcagcaggaa tcggaaacat ccacggaaaa
     5281 tacccagcaa actggaaggg actcagcttt gagactcttn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 ngctgacatg attaagaaag caatcgacct tggagtatct aagatcaacg ttaacacaga
     5461 gtgccagctt tcattcgcag atgcaacacg taaatacatc gaggccggaa aggatcttga
     5521 gggcaaggga tttgaccctc gtaagcttct tgctcctggt gctgaggcaa tcaaggcaac
     5581 agttaaggag aagatggagc ttttcggatc agttggaaag gctgaataat attattgtaa
     5641 tttagtattt ttgcacatac tactttacaa aatatttata tcttgtagta aaattttata
     5701 aaacttaact tttttaacat aaacgcttga tttttgatac tcaaaggagt atcttaattt
     5761 cagaaataat gttaagcgaa atgaacgagg gcgttccacc atagatggag tgccctcttt
     5821 tttaataaac taagaagtcc ggcataaaac aagaatattc gagtttatat gttgtgcagc
     5881 tgtttaaaca ttatttgaaa ataacaattc actcaagggg attgatgaac agaaaggatg
     5941 taagatatga gcgaacagga atatttcaac gtagcagaca tctttggaga gaacgtattt
     6001 aacgatgcag ttatgcagga gcgtcttcca aagaaggttt acaagaagct ccatgaggtt
     6061 atggacgaag gaaaggagct tgatctcgag actgctgatg ttatcgcaca cgagatgaag
     6121 gaatgggcca ttgagaaggg tgcaacacac tatacacatt ggttccagcc acttacaggc
     6181 gttactgctg agaagcatga ttcatttatc acttctccaa tgccaaacgg aaaagtactt
     6241 atgagctttt caggaaaaga gttaataaag ggtgagccgg atgcttcatc tttcccatca
     6301 ggtggtcttc gtgctacatt tgaggcaaga ggatatacag cctgggattg tacatcacct
     6361 gcatttgtca gacaggatgc agcaggcgca acactttgca ttccaacagc cttctgttca
     6421 tacacaggcg aggctcttga tcagaagaca cctcttctta gatcaatgga agcaatcaat
     6481 gatcaggcac tgagacttct tagattattc ggaaatacaa catcaaagaa ggttacacct
     6541 tctgtcggac ctgagcagga gtacttcatc gtagatcgtg agaagtattt acagagaaag
     6601 gatctcatct tcacaggacg tacacttttc ggagctatgc ctccaaaggg acaggagatg
     6661 gatgatcatt atttcggagc tatccgcgag agaatcgcag cttacatgag agatgtaaac
     6721 aaggagctct ggaagcttgg tgtttcagcc aagacacagc acaacgaggt agcacctgca
     6781 cagcacgagc ttgcacctat ctatgctgag tgcaacgtag ctgttgacca caaccagctt
     6841 atcatggaga cactcaagaa ggtggcaggc cgtcacggac tccagtgctt acttcatgag
     6901 aagccattcg caggagttaa cggttcagga aagcatgaca actggtcaat cacaacagat
     6961 gatggtatca acatgcttga gcctggcaag acaccacatg acaatgtaca gttcttactt
     7021 gtactcacat gtatgttaaa ggcaatcgac gagcatgcag cacttcttcg tgcggcagca
     7081 gcagatgtag gaaacgatca cagacttgga gctaacgagg caccacctgc aatcctttct
     7141 atctaccttg gagaccagct tggagacgta ttgaaccagc ttatctcaac aggtacagct
     7201 acacacagct taaagggtga gaagcttgag acaggtgtta agacaattcc tgatttcatg
     7261 aaggatgcta cagaccgtaa ccgtacatca ccattcgcat tcacaggaaa caagttcgag
     7321 ttccgtatgg ttggttcaca ggattctgtt gcacaggcca acatcgtgct taacacaatc
     7381 gttgctgagg cattctcaga cgcttgtgat atacttgaga aggcagacaa ctttgacctc
     7441 gctgtacatg atctcatcaa ggattacgca gtagagcacc agagaatcgt attcaacgga
     7501 aacggatact cagatgagtg ggtagctgag gcagagagac gtggacttcc aaacatcaag
     7561 tctatggtag atgcaattcc tgcatacgtt gctcctgagt cagttgcagc attcgagaag
     7621 ttcggtgtat ttacaaagac agagcttgag agccgtgccg agatcgagta tgagacatat
     7681 gcaaagacta ttaatataga agcaaaggct atgattgata ttgccggaaa gcagattatt
     7741 cctgcagtta tcaagtatat tacatcactt gcacagtcag ttaactcagt taagagtgca
     7801 gttgctgatg cagatgtatc tgttcagtca gagcttctta cagagagctc agctcttctc
     7861 tcagatgcac aggttgcact tgcaaagctt aaggatgaga cagcaaaggc cggtgctatg
     7921 gaagagggca gagagcaggc tgtttactac agagatgttg taaagactgc tatggatgct
     7981 cttcgtactc cggttgatga gcttgagatg attgtcgaca aagagatgtg gccaatgcca
     8041 tcatacggcg atttaatttt cgaggtttaa tccttgtagt ttcaggacca aagcttacgg
     8101 gaagctgttt agagcggtat atatgattta atttaacata ataataaaat ggagacattt
     8161 tgctgaacca cgaagctgtg gaaattggtg aagtgtctct gtttttttat aaattttact
     8221 ttcaataaaa tgaaaaaatg aaagagagga cattactatg acagaagaga ttagtggagt
     8281 aattttcgga gtctggttcc ttattggagc agcattggtt ttctggatgc aggcaggttt
     8341 tgctatggtg gaagcaggtt ttaccagagc aaagaatacc ggtaacatcc ttatgaaaaa
     8401 cctcatggat ttctgtattg gtacagttgt attcatactg attggttttt cgcttttatt
     8461 gggtgaagat gttcttggtt tcatcggtaa gccgggattt gacatcttta catcttacaa
     8521 agattttgac tggtcaaact ttgtattcaa cctggttttc tgtgcgacaa cagcaactat
     8581 cgtatcagga gctatggctg agagaacaaa atttatttcc tactgtgttt attcaggagt
     8641 tatttcagca cttatttatc caatcgaggc tcactggatc tggggtggtg gctggctttc
     8701 acagattggc ttccatgatt tcgcaggttc atgtgccatc cacatggttg gtggtatcag
     8761 tgctcttgta ggagctgcaa ttcttggacc tcgtatcgga aagtttacaa aggataaaaa
     8821 cggaaagatt acaaaagtaa acgctattcc gggacataac atcacaatcg gtgcactcgg
     8881 tgtattcatt ctctggcttg gctggtatgg attcaacggt gcagcagcaa aatcagtaga
     8941 gcagcttggt tctatctttg taacaacaac aatcgcacct gcacttgcaa cagtagtatg
     9001 tatgattttc acatggctca aatatggcaa gcctgatgtg tcaatgtgtc tgaatgcttc
     9061 acttgcagga cttgtagcta tcacagcagg ctgtgatgta acagatgcac ttggtgctat
     9121 cattatcggt tccgtagcag gacttctcgt ctgcttcggt gtatggttcc ttgacaatgt
     9181 acttcgtgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnttct acggcggtgg atttaagctt ttaggactcc
     9361 agctcacagg atttgtttct gttgcagcat ggacagtagt tactattaca atcgtattcc
     9421 ttgttatcaa ggctacactg ggacttagag tttcagagga agaggaaatc gtaggacttg
     9481 acccaacaga gcatggtctt ccatcagcat acgcaggatt tgctatcatg gatgtatcag
     9541 gtgatgttat ggatgtcaat gaaaatacag accttggttc ttctgagtat gcaacagctt
     9601 ctcaggcaaa gaaggatgca gcagtgcctg ttgtaaatgc aacaacaccg gcttcagcaa
     9661 gcggtatcca caaggttgtt attatttcaa agcttacaaa gtacgacaag cttcgtaagg
     9721 ctatgaatga cttaggtgta acaggaatga cagtgacaca ggttatgggc tgtggtatcc
     9781 agaagggtgc cggcgagaaa taccgtggag ctgagcttga tgcgacatta cttccaaagg
     9841 ttaaggttga ggtaatcgtt ggcaagattc cggtagaaaa ggttattgag actgctaaga
     9901 agactctcta caccggacat atcggagatg gcaagatctt cgtttacgac gtggcacagg
     9961 ttgtcaaggt tcgtacaggc gaggaaggaa ttgaggcatt acaggatgta gagtaatgca
    10021 aattattatc taatcccctt agcatatata catcaacaaa aacccccgag gatatgatcc
    10081 ttgggggttt tgctttagat aattaagttg taaatcgcaa tggttaacga tgagtgttac
    10141 cggatgaacc ggagctgccg gtatcagtac cacctgaacc tgagccaccg gtaccggaag
    10201 caccggctcc agagccgctt gaaccggaac cgccagtgcc agtaccggag ctaccagtac
    10261 cagcacctga gctagcaccg gtaccagttt cagccccacc tgtctgtgct ccagccgcag
    10321 tgccatcagt actgcccggt gtagtaccgg aattggtagg atccgtggtg gttggtgagc
    10381 tgttaatcag cgatgttgca tcatgtaatg tacatacggt attcatgaac gtatcatctg
    10441 ccatgaattc atattcaggg ctgcctgata cagcgcctgt tataaatacc tttgatgttt
    10501 tattcggaca atatggaccg gcaatggctc ccgaatcggc acaaatctca agtgcaacat
    10561 gatggtcgca gctgtctgtc ggcacagtgt ccgtatcaaa atattcagtt gtcagcattg
    10621 atcctcgagg atcattgtca catgttccct ctaaaggaac aagccctgat ttgctgcata
    10681 ccgtggtatt tgttatacca tccggctgta caaagtcttt ggtttctagt ccttcatgga
    10741 ttctgctcat ggcattcctc catatattct tagggtagga tgtgagcttg ccggactgtt
    10801 ttgagttgtc atcatagccg ccccatacga cacatgtgta gtatggtgtg tagcctgccc
    10861 acaggcagtc gcggttgctg gttgtggtac cggatttacc cgcctgggct atctggctgt
    10921 caaagcgcgc cagctttccg gttccctgtg tcagaacatc cttcatggca tctgtaagaa
    10981 gccatgccgt agtatccttg attacagtac gtgtgttctg tgtagtggtc ttgtcaagta
    11041 atacattgcc atcgtggtca agaacctgag tgtagaactt tggctctaag tatactccgc
    11101 cgttggctat agtagcatat gcacctgtga gctcaaggtt tgtgacaccc tttgtcagtc
    11161 cgccgagcgc cagtgacagg tttttgtcgg aatcggtgag agttgaaata ccgaaatcct
    11221 ccgcatattt gtaaccgaga tcaaccccga tatcctgtaa ggttttgact gttacaatat
    11281 tgatggactt tgttattgcc catcggatgg aggtaaagcc tccgaaagat ttgtcatagt
    11341 tgttaaaggt ttttgaacct actgtaaacg gtgcatcatc ctgcacggat gcaagtgttt
    11401 tgccgcctgc atcgagagca gcagcatagc agccgattat cttgaaggta gagccgggct
    11461 gtctgcatgt atctgtggct ctgttccatg ttctgttgcc ggatttgtca ccgcgaccgc
    11521 cgaccagtgc ctttacttca ccggtgctct ggtcgatgac tgtcatggca acctgtggtt
    11581 ccagcgtgat attgacagct tcagaaccct ccacaattga atcgccctcc tcaagtacat
    11641 cctgctcgta ctgtgcaata gctgcattgc attcatcagg gcttgagtag ttgatgctga
    11701 aatcatcatt gttggttttt ttcttataaa aagagagcat ggtctggttc gtgtaggtct
    11761 tgtacgaacc gtctgccttt tttacttcga aggaaagctg gaacgaatac tttgcgtcgg
    11821 acggatagtt ggatggattg tttgcctcct cgtcgcatat agtctgcata gtaaggtcct
    11881 gggtcgattt gatagtcagt ccgcccttgt aaatcagctt gtaggcttct gtctcagagt
    11941 aaccggcggc tgtgagatcg tcgaacacgt tgtcgatgag tgcatcaata aagtatgtgt
    12001 tcacagagcc tgagcctgtg gtgttgtact cagcaattct tgagtagaca tcatcggcca
    12061 tggcctcatc atattgcgcc ttggagatat agccctgatc cttcatgtta tttaacacct
    12121 tttcacgtct cttggcattt ttgtcagggt gtgtgatagg gttgtagccg gatggattct
    12181 gagtgatacc tgcgatgaca gatgcttcag aaagcgtgag ctctgagaca tccttgttaa
    12241 agtacttctt ggatgcggcc tgcacaccga gggtatttgc tcccagattg accgaattca
    12301 tgtagttttc aagaatccag tccttgtcgt ttacctgctt ttcgagctca agggcaaggt
    12361 actgctcctg aatctttctc tgaagctttt ctacaaagga agtttcgctt gtccagctgg
    12421 tcagtacgtt gttcttgatg agctgctgtg ttattgtgct ggctccctca ctgaatctgc
    12481 cctttgacag accggagata aaggctcttc cgataccatg caggtcgatg ccgttgtggt
    12541 cgtaaaagcg ttcatcctcg attgcaacaa aagcatgctg caaatccagc ggaatctcat
    12601 cgatggtgac atactgcctg tttgccccct gacctacgag tgtggcggtt tcttctccgt
    12661 ttgccgccag aacggtagtt gagtagccgg taggagtgac atcgatcttt gatatgtcag
    12721 gtgcggaatc aatgattccc ttcatgacac cgattcctga actgatgccg gctgcgcctg
    12781 ctacaattac tacaaccaga agaatcttta gaacaatgag cttgaacttg tttttgacct
    12841 tagagctctt attgtcatat ttccggagca gctgcattgt agcttttttg ccgtaattca
    12901 tatataggcc tccttgtcat tataaagatt tgtaatcttg tttgtatata ctgtgattag
    12961 aaaataaaca gtatacatta ttatatcaaa tatagcattt aaatgcaatt tacagattgg
    13021 ttttcatatt atataataca ggtaacaact ataaagtgtc tttatgatgc atgagtgtaa
    13081 tcaggcaata tcaggatgga aagaatatca tatgtcagag aatactataa gcctgtacgt
    13141 gtacaagggc ggacagggtg agataacaga gaagaaatcg aggtttatag caaccgtaag
    13201 gccggtagag tctgaggatg aggctgtaag cttcatcaat gagacgaaaa agaaatactg
    13261 ggatgcaagg cacaactgca gtgcctttgt tataggaaag agacaggagc tgaccagatg
    13321 ctcggatgac ggggagcctg caggcaccgc aggtcgtcct atgcttgatg tactccttaa
    13381 ggaaaatatc cacaatgtcg ctgttgttgt aacgaggtac ttcggtgggg tgctgcttgg
    13441 cactggcgga ctggtgcgtg cgtatcagca agccacaaag gcggggctat cggcaagtga
    13501 gataatagag aagaaggatg gtgcagtact gtttatcagg acggactata ccggaatcgg
    13561 aagacttcag tatctgtttg cacaggagaa gattacggtg atggacacag cgtatgaggc
    13621 ggatgtgctc gtgaaagccg ttatcccgga aaatgataaa aaacgcatag agaaaacaat
    13681 catagagcag acaaacggca cggcaaagct tgagtggggc gatgaggtga catttgctga
    13741 atatgatggc gaggtgttgc tgtttaaaaa ttaaccggtg ctcacatttt agcaggacaa
    13801 gcagaattta tattcaatta cattcaatta aaataaaaaa atgcttgaca ctatataatc
    13861 acatgtgtat aatatctttt gttgagacaa cacattggcc catcgccaaa tnnnnnnnnn
    13921 nnnnnnnnnn nnnnnggtaa ggcaacggac tctgactccg tcatttcaag gttcgaatcc
    13981 ttgtgggcca gctaaagaga acttcgatag aagttctctt tttttcttgt gtaataatgc
    14041 gatttgttat aaaataaaat cgtatatggc ttaaacagtt atagaaggat ggtgtaatat
    14101 gaacaagaat tttttgagaa ttattaatct tatagaagaa ctcggctctg aaaaaaagac
    14161 accaattact atacagcaat accaggatat cattaataaa agctcaaatc tttggatgtc
    14221 caatggtgtg gatgaagcgt ttagatttat cagaagttac ttcaatttta tagactagag
    14281 gaattagata tggctttttc atttgatagc agaatacgtt acagcgaggt ggattcgtca
    14341 tgcagactga gccttacggg gcttaccaat tattttcagg actgtagtgt gtttcactca
    14401 cagtcacatg atgtgggaat cagatttctt gcggacaatc atatagcatg ggtgctttca
    14461 tcatggcaga tatgcataaa caggcttcca ctgcttaatg aacaggtaaa gatatccaca
    14521 tgggcatatg gtatgaaggc gttttacggc tacagaaatt tcacacttga agatgcaggt
    14581 ggcagcacac tggcatatgc caattctgtc tgggtacttg ttgatacacg aaccggcaga
    14641 ccggtgaagg tgcctcagga gtttgcggat acatacggtc ttgagccgca gcttgagatg
    14701 gagtgtgcaa agcgcaagct tcatattcct gatgatatgg agaaaaaggg tgagattgtg
    14761 gttcctcagt tttttataga cagcaaccat catatgaaca atgagaagta tgttatgctg
    14821 gcacagcagc ttctgccaaa tgattttgaa atatctgagc ttcgtgtgga gtacagaaaa
    14881 gaagcaaagc ttggtgatac agttatatca tacgtgaagt acacatcaaa gagtgtgaca
    14941 gtggtgcttg cggatacgga taaaaaaccg tattcggtgg tggagttttt gagtaagccg
    15001 gtaccgggaa aataattata attccattat ttaacaacga tacagttttg atacagaaag
    15061 gacggaaaaa catgagatta ggagaatatc aggagcttgt atgtgtaaag aaggttgatt
    15121 ttggcatata t
//
LOCUS       DPUF01000252            2504 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_14668, whole
            genome shotgun sequence.
ACCESSION   DPUF01000252 DPUF01000000
VERSION     DPUF01000252.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_14668"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(1..1579)
                     /locus_tag="DHV81_08730"
                     /pseudo
     CDS             complement(1..1579)
                     /locus_tag="DHV81_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741445.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    258..338
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1563..>2504)
                     /locus_tag="DHV81_08735"
     CDS             complement(1563..>2504)
                     /locus_tag="DHV81_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HCI94516.1"
                     /translation="ASDISEELRQTVLDTAVEMGYSTKRSRKVENRKLALFIENMNYE
                     SIDEFGYDVVLGFKQNAFKHKWDVDIIPVTPQLQAEEKYDTYILKNGYCGAFLVGFAL
                     HDEWMQQLKNTTMPTVLFDNYIENNPNVAYVGTDSYEGIDMAVKHLHNLGHKKIAFIN
                     GSLFSLVSDQRQEAFENSMRDAGLEIYPELMGHGYYIPETAQYYVSNFIAAGATAILC
                     GSDTIAKGVILECQLHGFNVPNDISVVGFDDVKFAAALTPPLTTIRQERNDLGRCAYI
                     ILNSLIHHIPISRTQLRPMLIERESTARVSTAHAAEE"
BASE COUNT          625 a          525 c          565 g          708 t
ORIGIN      
        1 agagcgaccg acagcttatc cttgttctta aattgatgga tatcgtagta atcaattctc
       61 tttggcacct caggcccgaa atatatggaa aagccgtatt tctgttcaag cgctgtgata
      121 cgtgcattta tgtcggataa atcaccccag tcatatgacg cggtgtcagg tatcagggtg
      181 acctcgtcgc cgtatgtatt gtagtcggaa tccttcccgg cctcttccat agcttcctga
      241 ttttcatcgg tactgtcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag cagtgtatca cagtcattgt
      361 atgacgtgat ttcggctttg ctgtcattct tgcccttgtc gagtgaccag atgagaattt
      421 ccgggttgca ctgcgctgtg tacaaaagca tcatcacaca gtttgcatca ggcagatata
      481 ccgagttggc agaacagtag gtcttggtat cggctgccgc agagtcggat gagctgtcgg
      541 acattttgcc tgtgcttcgt gttgtcgggt tgtatgaaaa ggatgaactg acaccggtgg
      601 cgggtgaata tttatagtaa ctgatgatgt tgtccgagga taaatcctgg catcgtatga
      661 gcatgtcacc atcctctgcc agagtcacat ccaccacatc cggcagtgaa aaaaagctgt
      721 tttctccatc ggatgaatga tagttccaaa tattgttggc cgtatctgcc tcgattacaa
      781 agccctttga gtcgacacac gacatggaaa aactttgtcc ttcgtaggtt gaaagcactg
      841 tgttatcgtc aatccttata tcagagactg catatttgta ggtatcgggt gtgacacctg
      901 acacgagagc gtattttccg tcagcggagg tggcaatctg tgagacatca taataattgc
      961 cgggattcgg cacatcgcca tcatcagaat ctgatgtatc gcaattgcca tcatagtcct
     1021 caggattata tttgcaggtt tttatctcct ttaaatcctt atcatagatt gttgccctgc
     1081 cggcatcgga attgatgagc cagagtcggt caccttttat catgtagtca tcacagtcgg
     1141 ttttgtcgtg agtgaggctt gccgtgattt cattggacca gggattatat acatcaaatg
     1201 aatactgaac agattgatca tcggtctcta tgatggaatt attgtagctg ccctgtccta
     1261 ccataaggat gttatcacca aactgtcttg tatcattgta cggctgcccc tcaaaggcga
     1321 cattgtgcag gatagacaga ttgctgcttc ctttaacact tgtggtatcc tcccaaaacg
     1381 caggtgattt gtgcagaata gcctgctttt gtgcgggact cttaaataga taagagacat
     1441 ccgaaaaaga gcaggctgtc atggagattg cactgaaaaa taccagcgaa tatgtaatta
     1501 aagtggttac atgtttgcgt ttgaacaatt ttgactcctt tggaaggtgg tgtatatgca
     1561 ggctattcct cagcagcatg tgcggtggaa actcgtgcag tagattctct ttcaatcagc
     1621 atcggacgta gctgtgtgcg gctgattgga atatggtgaa tgagcgaatt caatataata
     1681 tatgcacagc ggcccagatc attacgctcc tgtctgatag tggtaagagg aggggtaagg
     1741 gctgcggcaa acttgacatc gtcaaagcct accacactta tatcatttgg cacattgaag
     1801 ccgtgcagct ggcactctaa gataacaccc tttgcaatag tgtcgcttcc gcagaggata
     1861 gcggtggcac ctgctgcaat aaaatttgaa acgtaatact gtgcggtttc aggaatatag
     1921 tagccgtgtc ccatgagctc cgggtagatt tcaagaccgg catcgcgcat agagttttca
     1981 aaggcttcct gacgctgatc tgaaaccagg gaaaaaagag aaccattgat aaaggcaatc
     2041 tttttatgac ccagattgtg cagatgcttt acagccatgt caatgccctc gtagctgtcg
     2101 gtgcctacat aggctacatt gggattgttt tctatatagt tatcaaacaa aacggtaggc
     2161 attgtagtat ttttgagctg ctgcatccat tcatcgtgaa gagcaaaacc taccagaaat
     2221 gcgccacaat agccattctt taatatatat gtatcatatt tttcttctgc ctgaagctga
     2281 ggtgtgacag ggatgatgtc cacgtcccat ttgtgcttaa aagcattctg tttgaaacca
     2341 agcactacat catatccgaa ctcatcgata gactcgtagt tcatattttc aataaacaga
     2401 gccagcttgc ggttttctac ctttctggag cgctttgtgc tgtagcccat ttcgacagca
     2461 gtgtctaaaa ctgtctgacg aagctcctca cttatatcgc tggc
//
LOCUS       DPUF01000253            8144 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_19450, whole
            genome shotgun sequence.
ACCESSION   DPUF01000253 DPUF01000000
VERSION     DPUF01000253.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8144)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_19450"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="DHV81_08740"
     CDS             <1..777
                     /locus_tag="DHV81_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI94517.1"
                     /translation="YDVPDGSISFKHVTFRYSDTAETPVLSDINLDIKSGETIGIIGG
                     TGSSKSSFVNLISRLYDTDTGSVIVGGHDVREYDMDTLRNKVAVVLQQNVLFSGTILE
                     NLRWGDKNATTDECIKACKMACADDFIESFPDKYNTYIEQGGTNVSGGQKQRLCIARA
                     LLKKPRILILDDSTSAVDTATDSKIRAALAKTIPGTTKLIIAQRISSVMDADRIIVMN
                     DGKVDGFDTHENLLKNNEIYRDVYDSQTNGGGDFDEGGAA"
     gene            777..2669
                     /locus_tag="DHV81_08745"
     CDS             777..2669
                     /locus_tag="DHV81_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI94518.1"
                     /translation="MPGPRGPRGPKPKIKNPGKLFARLMGFIFKKYLPACIIVVICIF
                     VSVLANVQGTMFTKNLIDDYIVPLLKTGTPDYGPLLAAMGRVAVFYGIGVISTFAYSK
                     IMIYVSQGTIKNLRVELFSHMQDLPIRYFDSHAHGDIMSIYTNDIDTLRQLISQSLPQ
                     ILNSAITVVSVFVSMVILNIPLTVLTIVMVIVTTVVTKKFAGFSSRYFLAQQRDLGKV
                     NGFIEEMLNGQKVVKVFTHEQENIEAFDRINDELFESAYNANMYSNMLGPVNAQIGNL
                     SYVLCALAGGVMALSGFGGLTLGKLASFLTFNKSFNMPISQVSQQFNSIIMALAGCDR
                     IFSLLDEAPETDEGYVTLVNAKEENGKLTETPERTGLWAWKHTHQADGSVDYKKLEGD
                     VVFDDVDFGYVPEKIVLHDVDLFATPGQKIAFVGTTGAGKTTITNLINRFYDIADGKI
                     RYDGININKIKKADLRHSLGIVLQDTHLFTATVMENIRYGKLDATDDEVYAAARLANA
                     DTFIRQLPDGYNTVLTGDGANLSQGQRQLLAIARAAIADPPVLILDEATSSIDTRTER
                     IVQDGMDKLMHGRTTFVIAHRLSTVRNSDCIMVLEQGRIIERGSHDELISKKGKYYQL
                     YTGKTA"
     gene            complement(2740..3297)
                     /locus_tag="DHV81_08750"
     CDS             complement(2740..3297)
                     /locus_tag="DHV81_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCI94519.1"
                     /translation="MAKKILVVVDAQNDFITGALGNKECDVATNVIAEKIRSGKYDDV
                     IFTRDTHDENYLNTQEGKKLPVAHCIKGTNGWEVDARLKDAMKERGVNCDDITYIDKP
                     TFGSKELGELLAEYDNSDTEIYFCGFCTGICVISNVAIAKTFCKDAEVFVIENACACV
                     TPESHKTAIEAMKMIQVGIKGTPSC"
     gene            complement(3332..5062)
                     /locus_tag="DHV81_08755"
     CDS             complement(3332..5062)
                     /locus_tag="DHV81_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94520.1"
                     /translation="MTESFKLPVGIESFEEIRSDGYYYVDKTKLIEQLVDSRGKVNLF
                     TRPRRFGKTLNMSMLKSFFEIGAASELFDGLYISHNKKLCDDNMGKYPVIFLSLKNVE
                     GLDFQAAKYQMIEIIAKEAKRFAYLKQSDRLDIDDKSAYASLIRLEDGRYEMRDEALY
                     ASLQTLSELLYKHHCRKTVILIDEYDVPLDKAFQNGYYKEMVSLIRAIFGKALKTNEA
                     LDFAVLTGCLRVSKESIFTGLNNFKILSITDTRFDEHFGFTDEEVERLLEFYHMENRF
                     AETKEWYDGYHFGNVDVYCPWDVINHVDRIKDDPMAGPEAYWINTSGNELVKRFIDKA
                     GKTTRDEIERLIAGEAIDKQIRLDMTYDEIDNNIDNLWSVLFTTGYLTYTKISEDKKY
                     NLVIPNKEIKEVFKLQIQEWFRNKIFSNTEQLQDFWKAFKDGNTQIMEMYLNKVLSNS
                     VSVFDTKARNEEKESSYHNLLIGILSGNEDWLVKSNVEAGEGFADIIVETDDPDEGII
                     AELKYTKDFKAMEKSCEKALKQIKDRRYQEYLLNDDRQNIMYYGITFCRKRCKVLVEG
                     YNGDSEPDKA"
     gene            complement(5099..5410)
                     /locus_tag="DHV81_08760"
     CDS             complement(5099..5410)
                     /locus_tag="DHV81_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCI94521.1"
                     /translation="MDVNSTFEKTGIKCKVLQEIIQLAKKNCVEKVILFGSRARGDYK
                     ERSDIDLAFCGGSSSHFILDVDETTSTLLEFDIVDLDKPVRKELLESIKREGVVLYQK
                     I"
     gene            complement(5433..6566)
                     /locus_tag="DHV81_08765"
     CDS             complement(5433..6566)
                     /locus_tag="DHV81_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylhydrolase"
                     /protein_id="HCI94522.1"
                     /translation="MIGFHGIGNSHMFEDEEMGSYHPEIENGDSNGDNETGIIGALGN
                     KLEKTFKNSLSAMKSEHTEKVHSNAAVADEEVSIQGKILANLMGHITNDLAIGKKIKS
                     GELRKRMKEPAWLVPDCFVTEDIDMGGFTMKLLSSKENPNIERVILQLHGGGYMGAVR
                     NAYYVFAGLYNEVSEGCSVLTPDYRVAPEHPYPAALEDAVACYKWLLSQGWFGSQIVL
                     AGDSAGGGLAMCLTMYLRDHDMPLPAGIVAMSPWTDLTAGGESYETNYEKDPLFGNTR
                     DSLIYVNDYPGDHDKMDPYISPLFGDFRGFPPMLIQAGSIEMLLSDSVDAAAKARNQG
                     VKVRLSVYEGMFHVFQMGYLNFPESKRAWAEVKRFLKVIKEEE"
     gene            complement(6563..7267)
                     /locus_tag="DHV81_08770"
     CDS             complement(6563..7267)
                     /locus_tag="DHV81_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94523.1"
                     /translation="MTKCMKCGVYIADNTDKCPLCQQVVAPVELSSDNHQGTEGAFEQ
                     HGTRYPDVKGASRKYRLLENIILFLSIVGQIVLTTVDYMTDHEINWSFIVLLVVIYAN
                     VTLRLAIVGRNGYIFKIVSTFVFTVLFLEGIDLLTGASGWALTFVFPSAILAMVLATI
                     ILMIVNIRNWQSYMMMQLFLVLMSVIAMILVAVKVIWFPYLAMGAMGASLFLFLGTLI
                     IGDKRARTELKRRFHI"
     gene            complement(7260..>8144)
                     /locus_tag="DHV81_08775"
     CDS             complement(7260..>8144)
                     /locus_tag="DHV81_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94524.1"
                     /translation="RELTYQYLRLTHPEIAKLKGDSLTQGTSLNREDSFVKNYKSSKP
                     SGFNRQKAYLLKLEKVPDGEFGLIHGMMPVSQLKQVCHRYGVSINDYLVACFVWSVYQ
                     ECFHGMPNDMPVRVAVPVNLRPYFDSVTTKNFFVMISAEFRPQNSSYTFEEVLKIVTD
                     SINSQISKEHLEDLFSYSVSNQKNILMRPVPLFIKNLAMKAVYTQSALANTTTITNIG
                     NIKVEPEYEPYITGFYSFIPMSKGQPMKGTICSYKDTLVFTFSSILADTMIQRSFFKK
                     LVNDGVEVTIETNGEYYD"
BASE COUNT         2224 a         2021 c         1521 g         2378 t
ORIGIN      
        1 tacgatgtgc ctgacggaag cataagcttt aagcatgtaa ccttcagata ttcagacact
       61 gctgagactc cggttctgtc agatataaat ctagacataa aatccggtga gaccatcgga
      121 atcataggag gaaccggaag ctcgaaatcc tcctttgtaa acctgataag ccgtctctac
      181 gacacagaca ccggctctgt aatcgtcgga ggccacgatg tgcgtgagta cgacatggac
      241 actctcagaa acaaggtagc cgtcgtgctc cagcagaacg tgcttttctc gggcaccata
      301 ctcgaaaatc tgcgctgggg tgacaaaaac gctaccacag atgagtgtat aaaggcatgc
      361 aaaatggcat gcgccgatga ctttatcgag tccttccctg acaagtacaa cacctacata
      421 gagcagggcg gaaccaacgt atccggtggc cagaagcagc gtctgtgtat cgcaagagct
      481 ctgcttaaaa agccaaggat actcatactc gatgacagca cctcggctgt ggatacagca
      541 acagattcaa aaatccgtgc tgccctcgca aaaacaatac cgggaaccac taagctcatc
      601 atcgcacagc gtatctcatc cgtcatggat gctgaccgta tcatcgtgat gaatgacgga
      661 aaagtggacg gctttgacac tcacgaaaat ctgctgaaaa ataatgaaat ctaccgcgat
      721 gtctacgatt cccagacaaa cggcggagga gactttgatg aaggaggtgc tgcctgatgc
      781 caggaccaag aggaccacgg gggccaaaac ccaaaatcaa aaatcccggt aagctttttg
      841 cgcgcctcat gggatttatt ttcaagaaat atttacctgc ctgcataatt gttgtgatat
      901 gtatctttgt cagtgtactt gcaaatgtgc agggaaccat gtttaccaaa aatctgattg
      961 acgactatat agttccactg cttaagacag gaactccaga ctacggacca ctccttgcag
     1021 caatgggaag ggtcgccgta ttctacggca tcggcgtcat cagtacgttt gcatacagca
     1081 aaataatgat ctacgtatca cagggtacca tcaagaacct gcgtgttgag ctcttttccc
     1141 atatgcagga tctgccaatc cgatactttg actcacatgc gcacggcgat atcatgtcga
     1201 tttatacaaa cgatatcgat acactccgcc agctcatcag ccagagtctt ccgcagatac
     1261 ttaacagtgc aatcactgtt gtgagcgttt ttgtttcaat ggtcatacta aatattcctc
     1321 tcaccgtgct aactatcgtg atggtaatcg taacaacagt cgtgacaaag aaatttgcag
     1381 gcttctccag ccgctatttc cttgcacagc agcgcgacct cggcaaggtt aacggcttca
     1441 tcgaggaaat gctcaacggc caaaaggtag tcaaggtatt tacccacgag caggaaaata
     1501 ttgaagcctt cgacaggata aacgatgaac tctttgaaag cgcctacaat gccaacatgt
     1561 actccaacat gctcggtcct gtaaacgcac agattggaaa tctaagctac gtgctctgcg
     1621 ctcttgccgg cggtgttatg gctctttcag gctttggcgg tctgaccctt ggtaagcttg
     1681 cgagctttct gacctttaac aagagcttta acatgcctat ctcgcaggtc agccagcagt
     1741 tcaacagtat catcatggcg cttgccggat gcgaccgtat cttctcactg ctcgatgaag
     1801 cgcctgaaac tgacgaaggc tatgtcacac ttgtaaatgc taaggaagaa aacggaaaac
     1861 ttactgaaac acctgagcgc accggcttat gggcatggaa gcatactcat caggctgacg
     1921 gctctgtgga ctacaaaaag ctcgagggcg atgtggtatt tgatgatgtg gatttcggat
     1981 atgtgcctga aaaaattgta ctgcacgatg tggatctttt tgcaacaccc ggtcagaaga
     2041 ttgcctttgt cggcaccacc ggagctggaa agactaccat cactaacctt atcaaccgct
     2101 tctacgatat agcagacggc aagataagat atgacggcat caatataaat aaaattaaaa
     2161 aggctgatct gcgccactct cttggcattg ttttgcagga tacccacctt ttcacagcta
     2221 ctgtcatgga aaacatccgc tacggcaagc ttgatgccac agatgatgaa gtatacgcag
     2281 ctgcccgcct ggcaaacgcc gatacgttta tccgccagct tcctgacggc tacaatactg
     2341 tactgacagg tgacggtgca aacctaagcc agggccagcg ccagcttcta gccattgcgc
     2401 gtgccgctat cgccgatccg cctgtgctca tccttgatga ggctacaagc tcaattgata
     2461 cacgtaccga gcgtattgtt caggacggca tggataagct catgcacggt cgtactacat
     2521 ttgttatcgc acacaggctt tctactgtca gaaacagcga ctgcattatg gttttggagc
     2581 agggacgcat tattgaaagg ggaagtcatg atgagctgat ttcaaagaag ggaaagtatt
     2641 atcagcttta tacaggaaag accgcttaat tcagtgtcat agcatgacta cgaaaaccgg
     2701 gagaccggga atacggcaga gggcacaccc tcatgtgcgc taacatgagg gtgtgccctt
     2761 aatgcctacc tggatcatct tcattgcttc tattgctgtc ttatgacttt caggcgttac
     2821 gcaggcgcag gcattttcta ttacgaacac ctctgcgtct ttacagaatg tctttgcaat
     2881 cgccacgtta cttattacgc atattcctgt gcaaaagccg cagaaataaa tttctgtatc
     2941 gctgttgtca tattctgcaa gcagttcacc aagctctttg ctgccaaaag tcggcttgtc
     3001 aatataagta atatcgtcac aattaactcc acgctccttc attgcatctt taagacgagc
     3061 gtctacttcc catccgtttg tccctttaat acagtgcgct accggaagct tctttccttc
     3121 ctgagtattc agataatttt catcatgtgt atcccttgtg aatatcacat catcatattt
     3181 gcctgacctg attttctcgg caataacatt tgttgccacg tcacactctt tgtttccaag
     3241 agctcctgta ataaaatcat tctgtgcgtc cactactact aaaattttct ttgccataat
     3301 tattttctcc tacataataa ttccttcgaa gttacgcttt atccggttca ctatctccat
     3361 tatatccttc tacaagcact ttacatcttt ttctgcaaaa tgtaattcca taatacatga
     3421 tattctgtcg atcatcattt aacagatatt cctgatatct tctatccttt atctgtttca
     3481 gagctttttc acaggatttt tccatagctt taaaatcttt tgtgtattta agctctgcaa
     3541 taattccttc atccggatca tctgtctcca caataatatc tgcaaagccc tctcctgcct
     3601 caacatttga ctttaccagc cagtcctcat tgccactcaa aatgccaata agcagattat
     3661 gataggagct ttccttttcc tcatttctcg cttttgtgtc aaaaacgctc accgaattgc
     3721 tcagcacctt gtttaaatac atttccatta tctgtgtatt tccatctttg aaagccttcc
     3781 aaaagtcctg caactgctcg gtattggaaa atattttatt tctaaaccac tcctgtatct
     3841 gcagcttaaa tacttcttta atctccttgt tcggaattac aaggttatat tttttgtcct
     3901 cacttatctt tgtatatgtc agataacctg ttgtaaacag aacactccat agattatcaa
     3961 tattattgtc tatttcgtca taggtcatat ccagacggat ttgcttgtct atggcttctc
     4021 ccgcgatcaa acgctcaatt tcatctctgg ttgtcttacc agccttatct ataaacctct
     4081 taacaagctc atttccgctt gtattaatcc aatacgcttc cggtccagcc attggatcat
     4141 cttttattct gtcaacatga ttgattacat cccacggaca atatacatca acatttccga
     4201 aatgataacc atcataccat tcctttgtct ctgcaaacct gttttccata tgataaaatt
     4261 ccagcaaacg ctctacttcc tcatccgtaa agccaaagtg ttcatcaaat cttgtatccg
     4321 taatagataa aatcttgaaa ttatttaatc ctgtaaaaat actttctttt gaaacacgaa
     4381 gacaacctgt taagacagca aaatccaatg cctcatttgt ctttaatgct tttccgaata
     4441 tggcacgtat caaagaaacc atctctttat aatagccgtt ctgaaatgcc ttatccagtg
     4501 gaacatcata ttcatctatt aaaataaccg tttttctgca atggtgctta tataacagct
     4561 cagataaagt ttgaagcgaa gcatacagtg cctcatctct catttcatat ctgccatcct
     4621 ctaatcggat taaagaagca tacgccgact tatcgtcaat gtccagcctg tcactctgct
     4681 tcaaatatgc aaatctctta gcttcctttg caattatctc tatcatctga tactttgcag
     4741 cttgaaaatc tagtccttcc acattcttta aagatagaaa tatcacagga tacttgccca
     4801 tattatcatc acacagtttt ttgttgtgcg atatataaag accatcaaat aattcagaag
     4861 ctgctccaat ttcaaagaaa gatttgagca tactcatgtt tagagtcttg ccaaaacgac
     4921 gcggtctggt aaaaaggttg acttttcccc ggctgtcaac caactgttca ataagctttg
     4981 tcttatcgac ataataatat ccatctgaac gaatctcctc aaagctctct attcccaccg
     5041 gtagtttaaa tgattcagtc atgcttgctc atctcctttc gaaataccaa tatccttctc
     5101 aaattttttg atatagcact actccctctc tttttattga ttccaacaat tcttttctta
     5161 caggcttatc caagtccact atatcaaatt ccaacaacgt tgaagttgtt tcgtctacat
     5221 ccaaaataaa atgactactg ctaccaccgc aaaatgccaa atctatatca cttcgctctt
     5281 tataatcacc tcgcgctctt gaaccaaaaa gaattacttt ttcaacacaa ttcttcttcg
     5341 ccagctggat aatctcctgt aatactttac attttattcc tgttttttca aaggtactat
     5401 ttacatccat aaatgctcct atattacttc atttattctt cctctttaat cacctttagg
     5461 aaacgcttga cctccgccca tgcacgcttg gactccggga aattgaggta tcccatctgg
     5521 aacacatgaa acatgccctc atacactgac aggcgcacct taacaccctg gtttcttgcc
     5581 tttgcagcgg catcgaccga gtcagataaa agcatctcta tcgagccagc ctgtatgagc
     5641 attggaggaa agcctctgaa atcgccaaac agcggcgata tgtatggatc cattttatcg
     5701 tggtcaccgg gatagtcatt cacgtagatg agactgtccc ttgtgtttcc aaacagtggg
     5761 tccttttcat agtttgtctc gtaggattct ccgcctgctg tgaggtctgt ccatggtgac
     5821 atggctacta ttcctgccgg aagcggcatg tcgtggtcgc gaagatacat ggtgaggcac
     5881 attgcaagac ctccgcccgc tgagtctcct gccagcacaa tctgcgagcc aaaccagccc
     5941 tgtgacaaaa gccatttata gcatgctacg gcatcctcaa gtgccgccgg atacgggtgc
     6001 tccggtgcca cgcgatagtc aggagtgagc actgagcagc cctcgcttac ttcgttataa
     6061 agtccggcaa atacatagta tgcattgcgc actgcaccca tatatccacc accatgcagc
     6121 tgcagtatca cacgctctat atttgggttt tcctttgaag ataaaagctt catagtgaag
     6181 ccgcccatat ctatatcctc tgttacaaag cagtccggaa caagccatgc aggttccttc
     6241 atgcgctttc tgagctctcc ggattttatt tttttaccga ttgcaaggtc atttgtgata
     6301 tgtcccatca gattagcaag tatttttccc tgtatactga cttcttcatc tgccacagct
     6361 gcattgctat ggactttttc cgtatgctca cttttcattg cagataatga gttcttaaat
     6421 gtcttttcaa gcttattacc caaagctcct attattccag tttcattatc accattacta
     6481 tcgccgtttt caatttccgg atggtagctt cccatctcct catcctcaaa catatgcgaa
     6541 tttcctattc catgaaagcc tatcatatat gaaatctccg tttcagctct gttctggctc
     6601 ttttgtcacc gataatcagt gttccaagga acaaaaacag cgatgcgccc atcgctccca
     6661 ttgccagata tgggaaccat atgaccttga ctgccacaag tatcatcgca atcacgctca
     6721 tcagcacaag aaaaagctgc atcatcatgt agctctgcca gtttcttatg tttactatca
     6781 tgagtataat tgttgcaaga accatcgcca gtatggcact tgggaaaaca aatgtaagtg
     6841 cccagccact ggctcctgtg agcaggtcta tgccctcgag aaacagcacc gtgaacacaa
     6901 atgtgcttac aattttaaaa atatatccgt ttcttccgac tattgccaga cgcaacgtca
     6961 cattcgcata gattacaaca agaagcacta taaagctcca gtttatctca tggtctgtca
     7021 tgtaatcaac agttgtcagc acaatctgtc ctactataga cagaaacaat atgatatttt
     7081 ccagtaatct gtattttctt gatgcgccct tgacgtcagg atatctcgta ccgtgctgtt
     7141 caaaagcccc ttcagtgccc tgatgattgt ccgatgacag ttcaaccggt gccacaacct
     7201 gctggcagag cgggcattta tctgtattgt ctgcaatata cacaccgcat ttcatgcatt
     7261 tagtcataat actctccatt tgtctcaatt gtcacttcca ctccgtcatt tacaagcttc
     7321 ttaaaaaagc ttctctgaat catcgtatcg gccagaatag atgagaaagt aaacaccagc
     7381 gtgtccttgt aggagcatat ggtgcccttc ataggctgtc ccttggacat tggtataaag
     7441 gaataaaagc ctgttatata cggctcgtat tccggctcca cctttatatt tccaatattg
     7501 gtgatagtgg tggtgtttgc cagtgccgac tgcgtataga ccgccttcat tgccagattt
     7561 tttataaaaa gaggcaccgg tctcatcagg atatttttct gatttgacac actgtatgag
     7621 aaaagatcct ccagatgctc cttcgaaatc tgtgaattga tggaatccgt cactatttta
     7681 agcacctcct caaatgtgta ggagctgttt tgcggtctga actcagcaga tatcatgaca
     7741 aaaaagttct ttgtggtcac ggagtcaaaa taaggacgaa gattcacagg aactgcaacc
     7801 ctgacaggca tatcgtttgg catgccgtga aaacactcct gatacacact ccacacaaag
     7861 catgctacca gataatcatt tatggaaact ccatagcggt ggcagacctg cttcagctgt
     7921 gatacaggca tcatgccatg tatcagccca aactctccat caggtacctt ttccagcttt
     7981 aaaagatacg ccttctgcct gttaaagcca cttggcttac ttgatttata gttctttaca
     8041 aagctgtcct ctctatttaa ggatgtgccc tgcgtcaatg aatcgccctt tagctttgca
     8101 atctccggat gtgtgagacg cagatactgg taggtgagct ccct
//
LOCUS       DPUF01000254            9634 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_19452, whole
            genome shotgun sequence.
ACCESSION   DPUF01000254 DPUF01000000
VERSION     DPUF01000254.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_19452"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="DHV81_08780"
     CDS             complement(<1..1238)
                     /locus_tag="DHV81_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HCI94525.1"
                     /translation="MKINRNMSAVRANKQLLGTENRLSKSMKRLSSGYKINSAEDNPA
                     GMAISNKMKAQIEALDQAKSNSSDAQNVMKIADGALGEVSSMLQRIRELAVQGASDTY
                     SENDKKALQSEIDELTKEVDRISGDTEYNTKSLLDGSSDTRVYIRQKDANGNMTQVNS
                     YITRINFSDNVASDKYELTVNSLATQATGSLASATKPDAEGTISINGVSVAVKADMTQ
                     DEYIQALQKAATTAGTTMQVNGDSISFLSNEYGSDSTVNISLSSSLKALAGAGYENAD
                     DGSGNMQLKSNGKDAVVTGGENMSDKTIRADGNRVYVVGNSGFSMDFLLSSDMDMTAG
                     SSNLQIDISDIGNMAVQIGANEGQEMKIRIPEVSSQTLYLDTVDVTVKGGADKAMSTL
                     DEAIEYVSGVRSRIGAFQNRL"
     gene            1408..1617
                     /locus_tag="DHV81_08785"
     CDS             1408..1617
                     /locus_tag="DHV81_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HCI94526.1"
                     /translation="MARVTKDTMIGDLLQIDQNVAPLLLNIGMHCLGCPSSQMETIEE
                     AAMVHGLDPDDLVVEINTFIDHDLG"
     gene            complement(1675..2415)
                     /locus_tag="DHV81_08790"
     CDS             complement(1675..2415)
                     /locus_tag="DHV81_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
                     /protein_id="HCI94527.1"
                     /translation="MKIEKVNDNQIRCTLTREDLEDRHIKISELAYGSAKAKSLFKDM
                     VEQANYEFGFETNDIPLMVEAIPLPSESIVLIITKVEYPDELDTRFSKFSEADDDFTD
                     LGNAGQPPVIKGAADILELFERIKKENEKTADSEQGTGDITEEQAEADVVKLFVFDKF
                     DDVRRISHVLDGFYSGRNDLYRDASSNKYYLVAHKSEHTPKEFNKVCNIISEYAYQKN
                     YVPANEAFFKEHGNVIIEEAAIQTLAAL"
     gene            2591..3955
                     /locus_tag="DHV81_08795"
     CDS             2591..3955
                     /locus_tag="DHV81_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HCI94528.1"
                     /translation="MKNTNHSLFDKYEMIKDLGTGSSGSVTLVRHISMDQERALKKVP
                     KASAFAESALSEARLLKSLEHPSIPRIFDFEDDDAFYYIVEEYIDGETLDSFLLHQQL
                     ISPGLFFNICEQLCNVFIYLHTQISTPIIYRDLKPEHIIVFHNKLKLIDFGVSTYVTR
                     KGNNFNHYGNIQFSAPECFTEDTITPVADIYSLGQLLQYILTFTPDSFSHKFQHIIQK
                     ATTSDASLRYVTVAELYQEIQEIQKLTGQAHLIHKIAVLGSHRGCGSTHVAVSLTCEL
                     NILGYHGIYIDSAKSILCHGNHDGLQIFKQKNGYYYYKSFQGIPDYSDGIQFNIPKDA
                     IQIYDLGTDVCNEKIYDMDLVLYVCNGSFWHLNDIHRNHSFLKKMTASLSVICNMCSR
                     QDASAIAALLNCSVYHFPYDSDMFKSSVQKETLIQKLLELKGGASLSDTLKGYLRKSI
                     LHHS"
     gene            4036..4506
                     /locus_tag="DHV81_08800"
     CDS             4036..4506
                     /locus_tag="DHV81_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94529.1"
                     /translation="MYRTAYVELNGTHEILSLSKTADEKVFTYKNVDFFPHAVMSTLT
                     EILNMRYERVIIDFGILNPNTLKEFMRCDVRIAVCTISKWNRGALNRIIELFNSNKIT
                     DSESVKILFNLVEKKKTINHKHFKYPVVGFPYLEDPFLINTSLFGTLDKISERN"
     assembly_gap    4579..4639
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5081..6814
                     /locus_tag="DHV81_08805"
     CDS             5081..6814
                     /locus_tag="DHV81_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18"
                     /protein_id="HCI94530.1"
                     /translation="MKKKLLPALIVTGLIILVVIIMGITALINKYTPTKKTEDLKEYF
                     NISSDDEAALVVDNELSDYKAKIIDKKIYVDYKFVHDSLNSRFYWDANENVLLYTTAS
                     DIISAAADSNSYSVTKQTNDFGYPIVKATSDSALIALDYVKQYSNITFKSYDDPARVV
                     ITSKWDEIDSATVNKSTQVRVKGGIKSPILKEVKKDDKITILDSGDKWDKVATEDGVI
                     GYIKRKALSESKKTKLTNPDYEEENFTHIKKDKDICLAWNQVTSQSANSKVAQVISST
                     KGINVMSPTWFYLNDNNGNLADIASKDYVSYCHSQGIEVWGLFSNFENTDVDAAYVLT
                     HTSTRTTLINQIMSAAFNYELDGVNIDFENITEDAYGDSYIEFIRELAIKCHNNGISL
                     SVDVTVPASFNKFFNRSDMANFADYIVIMGYDEHYKGSDAGSVSSIPWVTEGVESTLK
                     EVPADQIILGMPFYTRIWELTPKEDDDAVTDTEDQSKYTVASQVYSMDAAAAQLATNN
                     ATAALDEESGQNYAEWSVSNKLYKVWLEDNTSLENRLKLVDDNKLAGAAFWKLGFENS
                     SVWDTVIKYLN"
     gene            6967..7689
                     /locus_tag="DHV81_08810"
     CDS             6967..7689
                     /locus_tag="DHV81_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCI94531.1"
                     /translation="MKKYMYIIKKNKYCLILALFILSFYLGRGVCALAKNTGKNTNST
                     VIVIDPGHGGSDPGKISTSGVMEKDVNLSIAMALKQLFENRGFTVVMTRTTDCDLAPD
                     NSKHPKTDDLTKRTALMSKSNVAVSISIHQNSFEDSSSCGPQVFYYEASDAGKELASS
                     VLGALNTSLCVAKPRSIKPNKEYFILKKSTSPTVIVECGFLSNPAETLLLTDKNYQDD
                     LAHAIYIGVTDYLVQSPSGHLQ"
     gene            complement(7646..9634)
                     /locus_tag="DHV81_08815"
                     /pseudo
     CDS             complement(7646..9634)
                     /locus_tag="DHV81_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517493.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    8314..8385
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2687 a         2152 c         1765 g         2897 t
ORIGIN      
        1 agacggtttt ggaaggctcc tatccttgac cgcacacctg agacatactc tatcgcctca
       61 tcaagtgtgg acattgcctt gtctgcacca ccctttacag tcacatctac cgtatccaga
      121 taaagtgtct gcgatgatac ctccggtatc ctgattttca tctcctggcc ctcgtttgca
      181 ccaatctgca cagccatatt tccgatatct gaaatatcta tctgcagatt gctgcttccc
      241 gctgtcatat ccatatcact gctcagtaga aaatccattg aaaatccact gtttcctacc
      301 acatatacac ggttaccgtc ggctcttatt gtcttgtccg acatattttc tcctcctgta
      361 accacggcat ccttaccgtt acttttgagc tgcatatttc cacttccatc gtcagcattc
      421 tcatagcctg cacctgccag agccttaagc gatgaagaaa gagatatatt tactgtgctg
      481 tcgctgccat actcatttga taaaaaactg atgctatcac cattcacctg catagtagta
      541 ccggctgtgg tcgctgcctt ctgcaacgcc tggatatact catcctgagt catatctgcc
      601 ttcacagcca cactcacacc gtttatggaa atcgttccct ctgcgtcagg ctttgtggca
      661 gacgcaagac ttcctgttgc ctgtgttgcg agcgaattta ctgtgagctc atatttatct
      721 gacgccacat tatctgagaa attgattctg gtaatatagc tgttcacctg agtcatattg
      781 ccatttgcat ccttctgcct tatatagacg cgtgtatcac tcgaaccatc gagcagtgac
      841 tttgtattgt actccgtatc gcccgatata cggtccacct ccttggtgag ctcatctatc
      901 tctgactgca aagccttttt atcattctca gaatatgtat ctgaagcgcc ctgtaccgca
      961 agctccctga ttctctggag catgctgctc acctcaccaa gtgctccgtc tgctattttc
     1021 atgacattct gcgcatctga cgaatttgat tttgcctggt caagagcttc tatctgtgcc
     1081 ttcatcttgt tggagatagc cattcctgcc ggattgtcct cagctgaatt tatcttgtag
     1141 ccgcttgaaa gccttttcat agactttgat aatctgtttt ctgtaccgag gagctgctta
     1201 tttgccctga cagcactcat attcctgttt attttcatgt gttttccttt cctgataatt
     1261 gctcaggcat ccttgctgta gtattaacat tccgttgtct aataagtata tcgaactact
     1321 caaaaaaaat ctttatagta taatttgcaa ttaaatgcaa atcgtattat aataggcaca
     1381 ttaaaagact aataggagga aacaataatg gctagagtta ctaaggacac tatgattggt
     1441 gatcttcttc agattgacca gaacgtggca ccattacttt taaatatcgg catgcactgt
     1501 ctcggatgcc catcatcaca gatggagaca atcgaagagg ctgctatggt tcacggactt
     1561 gacccggatg atcttgtcgt tgaaatcaac acattcatcg atcacgacct cggttaattt
     1621 acgaaaagac ggcttgctta tgcaggccgt cttttatttg caatcaatat tctgttaaag
     1681 tgctgccagc gtctggatgg cagcttcttc aataatcaca ttaccatgct ccttgaaaaa
     1741 tgcttcattg gcaggcacat agttcttctg atatgcgtac tctgaaataa tattgcacac
     1801 tttgttgaat tccttcggag tatgctcact cttgtgggcc acaagatagt acttattgct
     1861 tgaagcgtcg cggtataagt catttctgcc gctgtaaaag ccgtcaagca catgcgaaat
     1921 cctgcgcaca tcatcaaatt tatcgaaaac aaacagcttg actacgtcag cctcagcctg
     1981 ttcttctgta atatcaccag tgccctgctc actgtcagct gtcttctcat tttccttctt
     2041 gatacgctca aacagctcca aaatatcagc agctcccttt atcaccggag gctgtcctgc
     2101 atttcctaag tccgtaaagt cgtcatctgc ctctgaaaac tttgaaaatc tggtgtcaag
     2161 ttcatccgga tattcaacct ttgtgatgat aagcactata gactctgatg gcagtggaat
     2221 tgcctccacc atgagtggaa tatcatttgt ttcaaagcca aactcgtaat tggcctgctc
     2281 taccatatct ttaaaaaggc ttttcgcctt cgctgatcca tatgccagtt cgcttatttt
     2341 tatatgtctg tcctcaagat cctctctggt aagagtacag cggatctgat tatcatttac
     2401 cttctcaatt ttcatatttt tcacatcctt ttccgaaaat atccgtgtaa atcgtaaaac
     2461 tattataata aacaccggtc atattgtaaa gcgcgaggcc tattaaaaaa aaataaaaaa
     2521 attgttgcaa ttattttaca ggtatgttat aagttatcca tgagatgtgc gctcgaccca
     2581 aaaggagcgt atgaaaaata caaatcattc tttgtttgac aaatatgaga tgattaaaga
     2641 tctcggaacc ggcagctccg gtagcgttac gcttgtcaga cacatatcta tggatcagga
     2701 gagagctcta aagaaagttc ctaaagcttc tgctttcgcc gaatccgcat tatcagaagc
     2761 aagactactc aagtctcttg agcatccgtc tataccacgt atatttgact tcgaagatga
     2821 cgatgctttt tactatatcg tcgaggaata tatcgacggc gagacattag attcattttt
     2881 gcttcatcag cagttaattt ccccaggttt attttttaat atttgtgaac aattatgcaa
     2941 tgtttttatc tatctgcata cacagatatc tactcccatt atttacaggg atcttaaacc
     3001 ggaacatatt atagtattcc acaataagct taagcttata gattttggtg tatcaacata
     3061 tgttacgcga aagggaaata acttcaatca ctatgggaat atacaattct ctgcccccga
     3121 atgctttacc gaggatacaa ttacaccggt ggcagacatt tacagcttag gtcagcttct
     3181 gcagtatata ctgaccttta ccccagacag cttttcccat aagtttcagc atattatcca
     3241 aaaagcaaca acatctgatg catccctgcg ctacgtgact gtcgcagagc tatatcagga
     3301 aatacaagaa attcaaaaat taaccggtca ggcacatctc atacacaaaa tagcagtttt
     3361 aggcagtcac agaggctgcg gctccaccca cgtggccgtt tcactcacct gcgaactcaa
     3421 tatccttgga tatcatggta tctacataga ctcagcaaaa agcatacttt gccatggtaa
     3481 tcacgacgga ctgcagattt ttaagcagaa aaacggatat tattattaca aaagctttca
     3541 gggcataccc gactacagtg acggtataca gtttaatatt ccaaaggatg ctatacagat
     3601 atacgacctt ggcactgatg tgtgcaacga gaaaatatat gacatggatc tcgtattata
     3661 tgtatgtaac ggcagctttt ggcatctgaa tgatatccac agaaatcatt cttttctcaa
     3721 aaaaatgaca gcttccctga gtgttatatg taatatgtgc agcagacagg atgcctccgc
     3781 gattgcggca ctacttaatt gcagtgttta tcactttcct tatgacagcg atatgtttaa
     3841 gagctcggtt caaaaggaaa cgctgattca aaagctgtta gaactaaagg gaggagcatc
     3901 actatcagac actttaaaag gatatttaag aaagtccatt ctgcaccaca gttagttggg
     3961 gtctgtggca gcgttcacaa tgtaggcaca acacatataa gccttgctct ttgtaattat
     4021 atgagcaata aatgcatgta tagaaccgcc tatgttgagc tgaatggtac acacgaaatt
     4081 ctttcactgt caaagacagc agacgaaaag gtttttactt ataagaatgt agatttcttt
     4141 cctcacgcag tcatgtccac actaactgag attttaaaca tgagatacga gcgtgtgatt
     4201 attgatttcg gcatacttaa tccaaacacc ttaaaggaat ttatgaggtg tgacgtaaga
     4261 atcgctgtgt gtacaataag taaatggaac cggggcgctt tgaacagaat tatcgagcta
     4321 ttcaattcaa acaaaataac tgattcagaa agcgtaaaaa tattgtttaa tctcgtggaa
     4381 aagaaaaaga caattaacca taaacatttt aagtatccgg tggtaggctt tccttactta
     4441 gaggacccgt tcctcatcaa taccagcctg tttggcactc tggataagat atcagaaagg
     4501 aattaatata tcacgatgca aaatgacatc tttaaccagc tgtttatcaa ttttatgtcc
     4561 gaagccttcg gagggcttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnna tcattatata tagacggagg tatttttata gaagaataaa
     4681 ctatatttat atattggaat ttttacagat attttatcaa attaaacgaa actaaaaatc
     4741 agcaattaaa ttgggtgtgg aaagctccgg aaatatcaat ttctataaac tatcaataat
     4801 taatgattaa taccattttt tctcaattat acaagaaatc gatgtcagca tatttttggt
     4861 tctttacccc gtttaaatgc aaaaagacaa ccaaaagaca tattttaaaa agaaaagcta
     4921 tgtgcgcggg ggaagctttt gctccttccg cgccaaaatg cataaaagaa tttcttaatt
     4981 tattacaaat ttatttcata ttatttgaat atgctatttc acaatggtat aattagctta
     5041 agcaatttcg acaccaaata ataggaaagg atttttacac atgaaaaaga aattattgcc
     5101 tgcacttata gtaaccggac ttattatcct cgttgtaata ataatgggta taactgctct
     5161 tatcaataaa tacaccccta cgaaaaagac agaggatctt aaagagtatt tcaatatctc
     5221 ttctgatgat gaggctgctc tcgttgtaga taatgagcta tccgattaca aggctaaaat
     5281 aatagataaa aaaatatacg ttgattacaa atttgttcac gactcattaa attccagatt
     5341 ttactgggat gcaaatgaga atgtgcttct ttacacaacc gcatctgata tcatctcagc
     5401 ggcggctgac agcaatagct acagtgtcac caagcagacc aatgactttg gctatccgat
     5461 tgtgaaagcc acctctgact ctgcgcttat cgctttagat tatgtaaagc agtactccaa
     5521 tatcaccttt aagtcatatg atgacccggc acgcgtcgtc atcacaagca aatgggatga
     5581 aatagacagt gccactgtaa acaagtctac ccaggtccgc gtgaagggcg gcataaagag
     5641 ccctattcta aaagaggtga aaaaggatga taagattact attcttgatt ccggtgacaa
     5701 atgggataag gttgccactg aggatggtgt tattggatat ataaagcgca aggctctgtc
     5761 cgagagcaag aaaacaaagc ttacaaatcc tgactacgag gaagagaact ttacacatat
     5821 caagaaggat aaggatatat gtcttgcctg gaaccaggta acaagccagt cagcaaacag
     5881 taaggtggcc caggtaattt catctaccaa gggtataaat gtaatgtcac caacatggtt
     5941 ttatttaaat gacaacaacg gcaatctggc agatatagca agcaaggatt atgtcagcta
     6001 ctgccattcc cagggtattg aggtatgggg actgttcagt aactttgaaa atactgatgt
     6061 agatgcagca tatgtgctca cacatacatc cacgagaact acactgatca atcagattat
     6121 gtcggctgca tttaactatg agcttgatgg tgtaaatatt gactttgaaa atatcacaga
     6181 agatgcatat ggagactctt acatagagtt tatcagagag cttgccataa agtgtcacaa
     6241 taacggcata tcactttcag ttgatgtcac agtacctgcc agcttcaaca agttcttcaa
     6301 tcgctctgat atggcaaatt ttgccgatta tattgtgatt atgggatacg atgaacacta
     6361 taagggctct gatgccggct cagtttcttc tattccgtgg gtaactgagg gtgttgaatc
     6421 cactctgaag gaggttcccg ctgaccagat tattctcggc atgccttttt ataccagaat
     6481 atgggagctc actccaaagg aggacgatga tgccgtaacc gataccgagg atcagtcaaa
     6541 gtacactgtt gcctcacagg tatatagtat ggacgcagct gcagcacagc ttgccacaaa
     6601 caatgccact gctgctcttg atgaagaatc cggtcagaac tacgctgagt ggtctgtatc
     6661 caacaagctg tacaaggtat ggctcgagga caatacatct ttagagaaca ggctcaagct
     6721 cgtcgacgac aataagctcg ccggtgcagc attctggaag ctcggctttg aaaattcatc
     6781 tgtctgggat actgttatca aatatctgaa ctgattgctt aaggcttact gaaagaggcc
     6841 gtacctttaa cagctacagt ctaactcagt accttttata ggttataaat aatatcagcc
     6901 aatcatataa tatacaaaca cacccgatgc cttctatgtg catcgggttt tttataggta
     6961 tcttatatga aaaaatatat gtacattatt aagaaaaaca aatactgtct tattttagct
     7021 ctctttattc tgtccttcta cctcggaaga ggtgtctgcg ctcttgcaaa aaacaccggc
     7081 aaaaacacta attcaactgt tattgtaata gacccgggcc atggtggaag tgatcccggt
     7141 aagatttcca cctccggcgt catggaaaag gatgtcaacc taagtattgc aatggctctt
     7201 aaacagctgt ttgaaaacag aggctttact gttgtcatga ccagaaccac cgactgcgac
     7261 ctcgccccgg ataattccaa gcatccaaag acagatgatc tgacaaagag aactgccctg
     7321 atgtctaaaa gcaatgtggc agtcagcatc agtatccatc aaaacagctt tgaggacagt
     7381 tcaagctgcg gtccacaggt attctattat gaagcctctg atgcaggaaa agagctcgca
     7441 tcctctgttc ttggagcttt aaacacttct ctttgtgtcg caaagccacg aagcattaag
     7501 cccaataaag aatactttat acttaaaaaa tccacatccc cgaccgtaat cgtagaatgt
     7561 ggattcttga gtaatcctgc tgagacactt cttcttactg acaaaaacta tcaggatgac
     7621 ctggcacatg caatatatat cggtgttaca gactatcttg tgcaatcacc ctctggacat
     7681 ttacaataaa ctgctctgcg atgtgatata gtctggttgt ctggactctt tcgctgccat
     7741 caggctgctt ttctgactca gtagccgatt tcgtatctga tgccataggc tcttttgcag
     7801 atatatcatg ctccaatgcc acgtctatgt gcacgctctg ttcaccatca cttattcctg
     7861 actcaaacgt agtgatattg tcttcaaaca gctttttggt ctgctcatcg tctgtgacta
     7921 tcatcgcaga tactgagttt tcctttgcct caaaatatgc agctcccctg ccgcattttt
     7981 tgtcctcaag gaatatgtca accaggccct ttttatcctc gccccttact atactaagct
     8041 tcatgcctgt cactccgtct gcagtctcta ttggtacaat atagttttcc tgtctcgaca
     8101 tatttgaagc tatccttagc tgactgcata atagcttcat ctgtctgagg tctattgaac
     8161 ctgtgtgcct atcctctata atcatcgaat ccatcacgtg ctctgctgta tcagccagcg
     8221 tttcctgcgc ctgcgcaagc tcctttggat ttttgaccga ctcagaaaac cgtctgagag
     8281 tctcatccaa aagagccctt gccgtagcac tgtnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaggtt taccgcggtt
     8401 gtttttacat cgtatttctc tagaaaagca tatacgttct caggcaccga aactgcctga
     8461 ttgaactgtc ttagctgttc tgtgacatat tgctctgaca gtgcgttatc ctcgtctgcc
     8521 tcatatacag cctgcctgag ctgctccgga gtcatctcaa gccagctgtc atcctgcaca
     8581 aactccattt tctcaggtga aagtgtatca agcacatctc ttagacagtt tgtctgatat
     8641 gcgctctcta tctgttcatc aattctagct cccttactta ctccttccac tccggcaaag
     8701 gtatcgtcca ccttatagtc catggtactc tttcctcttg tccggactgc tgtaagcaga
     8761 tttttcattg taatatctgc gccctgattg acaagtgagc ctatcacagc accatctgac
     8821 tgctccacct gtgatataag cctgtatata cctatatatg agtctctttc ctcctctgag
     8881 atttcatttt tcttttcaag cttccagaga aactcactga atttttcatc tctttcatca
     8941 ttattttctg attttatctg tctggctgtg tcacttatgt cgctcatgct catatcaagc
     9001 ggtgatatac ctctttttat catctcaagt gtcacggccg gtgtcatatc gctgaatgct
     9061 ctctgcacct gctcatctac agactttatc tgctttatat tgtcctctgt gatgtcggta
     9121 ctgttgtacg caagtatccg cacagccctg cgattgcttt caaatatatc tatctgaagc
     9181 tctttaagta tatcatccac attctgaaat gcttttgata tactgtctcc catatctgcc
     9241 cgcggtgatg tcataagagt ctcatatcct gaggctgctg cttctaatga ctgcttcatt
     9301 gctttgcctg ctgtatacag ctcatacaca gtgtcatcag cggattccaa ggtcagtaca
     9361 tacgccggct gctgcttcat ctgattgaaa atatcacata cattattgta catacgtacc
     9421 ctatcatcag atgcctctac actgctatca ccaaacagcg ctccgtagta tttattttcc
     9481 tgctgcttaa gcatctccac tacacgttca ataggctctg tgtctattga aactcctttt
     9541 tttatgagtg aaaggttggc ttctgctgtc atggaaagtc tcgcttcctc aagctttctt
     9601 tgagctgtca caagccttgc ttcccggctg tctg
//
LOCUS       DPUF01000255            5922 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_1491, whole
            genome shotgun sequence.
ACCESSION   DPUF01000255 DPUF01000000
VERSION     DPUF01000255.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_1491"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="DHV81_08820"
     CDS             complement(<1..560)
                     /locus_tag="DHV81_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HCI94532.1"
                     /translation="MECTDSLDEFSKLHTVLCYNLQWFAQDGEGGEKTEPATEKKLKD
                     AREEGKVAKSKELTAAFDLIVLFLVLKIFISFVGGNLIGLFSTVYKKMPDFLKESQEQ
                     ISSVSVTHYLNNVILQFLLTVLPFFAFGVVITILVSIIQVGFKISTKPMAPKLSKFNP
                     INGFKRILSKDSIFELLKSVLKVGII"
     gene            complement(547..1326)
                     /gene="fliR"
                     /locus_tag="DHV81_08825"
     CDS             complement(547..1326)
                     /gene="fliR"
                     /locus_tag="DHV81_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCI94533.1"
                     /translation="MINYSFSIANFEYFLLILVRIASFVYIAPFFGDNAVPARVKTGL
                     SAFVALFMYNIIERPQLSYVSAVGFAVLVVKEGITGLLIGFAANICNSIVIFAGNLID
                     MDIGLSMATEFNPSMNSETTVTGNFYYYVVLVLLLVSDMHTYLIRAVCDSFSVVPLGG
                     AQFQFDNMLSTMTKYMGDMFVIGFRIFLPVFACMMIMNCILGIMAKVAPQMNMFSVGI
                     QLKIIAGFIVLFLIVYLIPNIANFIITEIKTMVVSIIDGMY"
     gene            complement(1335..1613)
                     /gene="fliQ"
                     /locus_tag="DHV81_08830"
     CDS             complement(1335..1613)
                     /gene="fliQ"
                     /locus_tag="DHV81_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HCI94534.1"
                     /translation="MITQEAVLDIAREAIYTTIITSAPLLLISLIVGLIVSIFQTVTS
                     IQEQTLTFVPKIIAIFLVMMLCGSWMLDTMSGFMTDLWSKFSYYISMG"
     gene            complement(1625..2524)
                     /gene="fliP"
                     /locus_tag="DHV81_08835"
     CDS             complement(1625..2524)
                     /gene="fliP"
                     /locus_tag="DHV81_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HCI94535.1"
                     /translation="MNRFMKKFKAVYCNASFIFAIAVLMVTISLCIFYPDSVNASQSP
                     SPSGDISTNDVTNDLDDVKSLDSGDKDLADATSDSTNGLNIYYNNESGSLSTPVRMFL
                     VLTVLSLAPSILIMLTSFTRIIIVLHFVRTAIGTQTAPPNQVLVGLALFLTLFIMWPT
                     FSQVNEKAIKPLDEGKITQQEAIKEAEGPFREFMYGQTQTKDIKLFVDISGDEYDDYD
                     DVPFTTLVPAFILSELRTAFILGFLIYIPFIVIDMVVSSVLMSMGMMMLPPTTISMPF
                     KVLLFILADGWDLVIGNLVKTFY"
     gene            complement(2517..2903)
                     /locus_tag="DHV81_08840"
     CDS             complement(2517..2903)
                     /locus_tag="DHV81_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biogenesis protein"
                     /protein_id="HCI94536.1"
                     /translation="MILAATSLDSFVQLITVLIIFVFVLILTYFTTRWMAGIQKGRSF
                     NKNLRIIETISVGNNKMISIVEAGTKYIVVSIGKDDVNYLTELKEEELKDLSFKDPQS
                     MMQNPESFSAIMDKLKEKYSNRSRHE"
     gene            complement(2945..4159)
                     /locus_tag="DHV81_08845"
     CDS             complement(2945..4159)
                     /locus_tag="DHV81_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HCI94537.1"
                     /translation="MDGVLSQEEINALLNNNDAADSSDEILTESEKDAIGEIANISMG
                     TAATTLFSLVNRRVEISTPVVSFSNWDKIVDEYEKPCVFIKIGYTQGLNGSNILVLKE
                     KDVKIITDLMMGGDGTNINGEIGELHLSAISEAMNQMMGSSATSLSSMLNRTIDISPP
                     IAEVVDLKNTLNEGEIDSFLSEEFVKISFHMEIGDLVDSEIMQLYPPAFVKEMCKGVS
                     QNMELDTQSVTEDVPQSSNTATQAAPIQQPQDSGSMNNASANSQGMINENMMNSNMNS
                     QNMMNQGMMNQNMGAQNMIGNQVNVQNAQFTPFGAMPNQVYQPENIDLIMDVPLEVTV
                     ELGRTKKSISEILDFGPGKIIELNKLAGEPIDILVNGKYVAKGEVVVIEESFGVRITE
                     IVNSKINTGKTK"
     gene            complement(4163..5143)
                     /locus_tag="DHV81_08850"
     CDS             complement(4163..5143)
                     /locus_tag="DHV81_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCI94538.1"
                     /translation="MGDVLSQSEIDNLLNALSSGELDVDEIKENSEQTVKDYDFARPS
                     KFSKEHLRTLEIIFEHYGRLLTTNLPVYLRKSVQVEVMNSEAVTYSEFTNALSNPVLL
                     GIVNFAPLHGNIIVEMASGLGYAIVDRMLGGRGDSLDKTREFSEIELLIIERILVICI
                     NLLQEPWQNVLDISPHLERIETNSQYAQIISPSEVIAIITMNIKIGDVEGLMNICLPY
                     ITLESVIDKLNTRYWYSNIQNHDETNYRNAIESLIQKSQIPVKAVLGKSLISVKDFST
                     LVPGDVIRLDTNVDDELDIYVGNIKKFTALPGSSGDKYAVRITSVVREEQ"
     gene            complement(5222..5743)
                     /locus_tag="DHV81_08855"
     CDS             complement(5222..5743)
                     /locus_tag="DHV81_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94539.1"
                     /translation="MKKNLISVLILALCFANLVLTALLIFTIIPETKKANNLIDQVCQ
                     AISLDLNSGTATSGSQLPQDQIVDYALTADDDTLTFNFAPSEDGNTHYLVCGISLSLN
                     KKSDGYKTYGEDLSAKKNVILADITDIIGDYTMEQYNTDKSAVHDKILKTLQKKFGAD
                     YIVDVNIVSSLTQ"
     gene            complement(5761..>5922)
                     /locus_tag="DHV81_08860"
     CDS             complement(5761..>5922)
                     /locus_tag="DHV81_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCI94540.1"
                     /translation="IRDNTSLNPAHIKSSGRGDYDPVADNSTPEGRARNRRVEIKVYN
                     SYNSEFNTN"
BASE COUNT         1788 a         1290 c         1024 g         1820 t
ORIGIN      
        1 ataatgattc caacctttag cacagactta agaagctcaa agatggagtc ctttgacagt
       61 atcctcttaa atccgtttat gggattaaac ttgctaagct ttggagccat aggcttagtg
      121 gatattttaa atccaacctg gatgatactc acgagaatcg tgattacaac tccgaaagca
      181 aaaaacggca gcacagtcag taaaaactga agtattacat tatttaaata atgcgtgacc
      241 gaaacagatg aaatctgctc ctgtgactcc tttaaaaagt caggcatttt tttatataca
      301 gtagaaaaca atccaatcag attgccccct acaaaggata taaatatctt taatactaaa
      361 aacagcacta taaggtcaaa ggcagctgta agctccttgc tttttgctac cttgccctct
      421 tccctggcat cctttaactt cttttcagtt gccggctctg ttttctcgcc gccctcgcca
      481 tcctgtgcaa accattgcag attgtagcac aacactgtat gcaatttgga aaattcatct
      541 aaactgtcag tacattccat caattatcga aaccaccata gtcttaattt ctgtaataat
      601 aaaattagca atgttcggaa ttagatatac tatcaaaaat aatacaataa atcctgctat
      661 aatttttaac tggataccta cagagaacat attcatctga ggcgcaacct ttgccatgat
      721 tcccagtata cagttcatta tcatcatgca cgcaaagacc ggaagaaata tcctgaagcc
      781 tattacaaac atatcgccca tatacttagt catggtagag agcatattgt caaattgaaa
      841 ctgtgcacct ccaagcggta caactgaaaa ggagtcacaa actgccctta taagatatgt
      901 gtgcatatca gatacaagaa gcaggacaag aactacgtaa tagtagaaat tacctgtaac
      961 tgttgtctct gaattcatcg atggattgaa ttctgttgcc atggacagac ctatatccat
     1021 atcaatcaga tttcccgcaa aaataacaat ggaattacat atattggccg caaaacctat
     1081 gagcagtcct gtaattcctt cttttacgac cagtacagca aaacctactg cactgacata
     1141 ggaaagctgt ggtctttcta tgatattgta cataaacaaa gcaacaaagg ctgataagcc
     1201 ggtcttgaca cgtgccggaa ccgcattatc tccaaaaaac ggagctatgt acacaaatga
     1261 tgcaatacgc accagaatca gcaaaaaata ttcaaaattt gcaatagaaa aagaataatt
     1321 aatcatatat catcctatcc catgcttata taataactga atttggacca caaatcagtc
     1381 ataaatccgg acatagtgtc gagcatccat gagccacaga gcatcatcac aagaaaaatt
     1441 gcaattattt ttggcacaaa tgtaagtgtc tgctcctgaa ttgacgtaac tgtctgaaat
     1501 atactgacta taagtccgac aatcagcgaa ataagcaaaa gcggagctga tgtaatgatt
     1561 gtagtataaa ttgcctctct ggcaatatcc aaaacagctt cctgggtaat catatgtaca
     1621 cctcctaata aaacgtcttg accagattac caataaccag atcccagcca tccgcaagga
     1681 taaacaaaag taccttaaac ggcatcgaga tggttgtcgg tggaagcatc atcataccca
     1741 ttgacattag cactgatgaa acaaccatat ctattactat aaacggaatg tatattaaaa
     1801 agcctaatat aaaggctgtt ctaagctcac taagtataaa ggccggaaca agtgttgtaa
     1861 aaggtacatc atcatagtca tcgtactcat ctcctgagat atccacaaac agctttatgt
     1921 ccttagtctg tgtctgccca tacataaact ccctgaatgg tccctcagcc tcctttatag
     1981 cctcctgctg ggtgatttta ccctcatcaa ggggctttat agccttttcg tttacctgtg
     2041 aaaaagtcgg ccacataata aaaagtgtca aaaacaaagc caaaccgaca aggacctgat
     2101 taggtggtgc tgtctgggta ccgatagctg ttctgacaaa atgcagtacg ataataattc
     2161 ttgtaaaaga agtcagcatg atgagaatag aaggtgctaa agataatact gtaagcacca
     2221 aaaacatcct gacaggtgtg gacagagagc ctgactcatt gttgtaatat atgttcagac
     2281 catttgtgct gtcagaggtg gcatcggcta aatccttgtc accactgtca agacttttaa
     2341 cgtcatccaa atcgtttgtc acatcatttg tactgatatc accggatggt gaggggctct
     2401 gacttgcatt tacactgtca ggataaaata tgcataatga aattgttacc ataaggactg
     2461 caattgcaaa aataaatgat gcattgcaat atacagcctt gaatttcttc atgaatctat
     2521 tcatgtctgc tcctgttact gtacttttct ttcaatttat ccataattgc tgaaaaggat
     2581 tccggattct gcatcatgct ttgaggatcc ttaaatgata aatcctttaa ttcctcctcc
     2641 ttaagctcgg tgagataatt cacatcatcc tttccgattg aaacaacgat atattttgta
     2701 cctgcctcaa caatactgat catcttatta tttccgacgc ttattgtttc aataattcta
     2761 agattcttat taaagcttcg ccccttctgg atacccgcca tccatctggt ggtaaaatat
     2821 gtcaggataa gcacaaaaac aaaaattatt aaaactgtga tgagctggac aaagctgtcc
     2881 agtgatgttg ctgctaatat cattttaatt cctctgcata gtattgatta aatgtcagct
     2941 gaaactattt tgtctttccg gtgtttatct ttgaattgac tatctcagtg attcgtacac
     3001 caaagctttc ctcaattaca acaacctcgc ccttagctac atatttacca tttacaagaa
     3061 tatctatcgg ttctcctgcc agcttattaa gctctatgat cttgcccgga ccgaaatcga
     3121 gtatttcgga aatggatttt ttggtgcgtc caagctcaac tgtcacttca agaggaacat
     3181 ccatgattaa atcaatgttt tcaggctggt agacctgatt tggcatggct ccaaaagggg
     3241 tgaattgagc gttctgtaca ttaacctgat tgcctatcat attttgtgcc cccatattct
     3301 ggttcatcat tccctgattc atcatattct gactattcat attcgaattc atcatatttt
     3361 cgtttatcat gccctgactg tttgcggaag cattattcat gctgccacta tcctgtggct
     3421 gttgtatagg cgcagcctgt gtggcggtgt ttgaactttg aggaacatcc tctgtcacac
     3481 tttgtgtatc aagctccata ttctgagata cccccttaca catttccttt acaaatgccg
     3541 gtgggtaaag ctgcattatc tcgctgtcta ctagatcccc aatctccata tggaaggaaa
     3601 tctttacaaa ctcttcagac aggaagctgt caatttcacc ctcgtttaag gtgtttttta
     3661 aatcaacaac ctctgcgata ggagggctga tatcgatagt acgattgagc atagacgaaa
     3721 gagatgtggc ggaagaaccc atcatctggt tcattgcctc actgatagca ctaaggtgca
     3781 gctctccaat ctcaccattt atattcgttc catctccacc catcataagg tcggttataa
     3841 tttttacatc cttttctttg agtacgagaa tattgcttcc atttaacccc tgtgtgtatc
     3901 cgattttgat aaacacacac ggtttttcat actcgtcaac aattttgtcc caatttgaaa
     3961 aagaaacaac aggagtagaa atctctactc ttctatttac aagtgaaaac agcgtggttg
     4021 cagctgttcc catactgata ttggcaatct ctccaatcgc atccttttcg gattctgtca
     4081 atatctcatc gctcgaatcc gccgcatcgt tgttatttaa caatgcattt atttcctcct
     4141 gcgataaaac tccatccatg atttattgct cctctctgac tactgatgta attcttaccg
     4201 catatttatc accggaagaa ccaggtagtg cagtaaactt tttgatattt cctacataaa
     4261 tgtccaactc atcatccaca ttggtgtcaa gtcttattac atcacccggc acaagcgtag
     4321 aaaaatcctt tactgatata agactcttgc caagtaccgc tttgaccggt atttgtgatt
     4381 tttgaataag gctttctatg gcatttctat agttggtttc atcatgattt tgtatgttag
     4441 aataccagta tctggtattc agcttatcta taacagattc aagtgtgata taaggcagac
     4501 atatattcat caatccctca acatcaccaa tctttatatt catagtgata atagctatca
     4561 cctcgctggg agatataatc tgagcatact gggaattggt ctcaattcgc tctaaatgag
     4621 ggcttatatc aagtacattc tgccacggct cctggagcag attaatgcat ataactaaaa
     4681 tacgctcaat aatcaaaagc tcaatctctg aaaattctct cgtcttatcg agagaatctc
     4741 cccttccgcc aagcatacgg tctacaatag catatccaag accggaagcc atttcaacta
     4801 ttatattccc atgcaacggc gcaaagttga caatgccaag cagcaccgga tttgaaagcg
     4861 catttgtgaa ttctgagtat gtaactgcct ccgaattcat cacctctacc tgcactgact
     4921 ttcgcagata aacgggaagg tttgttgtaa gaagccttcc ataatgctca aatattattt
     4981 caagggtcct tagatgttcc tttgaaaact tagatgggcg ggcgaaatca tagtctttca
     5041 ccgtctgttc actattctct tttatttcat ctacatcaag ctcgccgctg cttagggcat
     5101 tgagcagatt gtctatctca ctctgagata aaacatcacc catctgttaa tcaccacctg
     5161 tatccaaatt attaaatatc tgcctgtttt aataccggca taagtatttg taaagaaatc
     5221 atcactgggt aagcgaagat acaatattta catctactat atagtctgct ccgaatttct
     5281 tctggagagt tttgagaatc ttatcatgaa cagcactctt atcggtgttg tactgttcca
     5341 ttgtgtaatc gcctattatg tctgtaatgt ctgctaagat aacatttttc ttggcggaca
     5401 aatcctcacc gtaggtctta tatccgtcac tctttttgtt gagtgaaagt gatatgccac
     5461 aaacaagata atgtgtattg ccatcctcag atggggcaaa attgaaggta agtgtatcat
     5521 catcggctgt aagtgcataa tcaacaatct gatcctgcgg cagctgtgaa ccactagttg
     5581 cagtgccact gtttaagtct agtgatatag cctgacacac ctggtctatc agattgtttg
     5641 ccttttttgt ctcaggtata attgtaaata taagcagtgc tgtaagcaca aggtttgcaa
     5701 aacaaagtgc taaaatcagt actgaaatta aattcttttt catatgcatt cctttccgga
     5761 ttaattggta ttgaactctg aattgtaaga attatacacc ttaatctcta cacgtctgtt
     5821 cctggccctt ccctctggag tagagttgtc cgcaaccgga tcatagtctc ctcttccgga
     5881 tgactttata tgggcagggt ttaagcttgt attgtccctg at
//
LOCUS       DPUF01000256            7684 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13715, whole
            genome shotgun sequence.
ACCESSION   DPUF01000256 DPUF01000000
VERSION     DPUF01000256.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13715"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="DHV81_08865"
     CDS             complement(<1..266)
                     /locus_tag="DHV81_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526221.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiC"
                     /protein_id="HCI94541.1"
                     /translation="MSNYTTQMDAARKGIVTPEIEKVAKKEKMDVDKLMELVASGKVA
                     IPANKHHKSLDAEGVGSMLRTKINVNLGVSRDCKDYDVEMQKVM"
     assembly_gap    284..352
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<353..1098)
                     /gene="thiD"
                     /locus_tag="DHV81_08870"
     CDS             complement(<353..1098)
                     /gene="thiD"
                     /locus_tag="DHV81_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCI94542.1"
                     /translation="MHTALTIAGSDSSGGAGVQADLKTMLANGVFGESVITALTAQNT
                     MGVDAVMNVPADFIEAQMKAVFTDIFPDAVKIGMTSSVDTIEAIAAGLVKYNAKNIVL
                     DPVMVATSGSRLLEENAANTLMEKLFPLADIITPNIPELEVISGRQIESTDDIIEAAK
                     AIYDKYGCPVLSKGGHFTDTACVCDYLWDGKQIITFETKRVDNPNSHGTGCTLSSAIA
                     SGLAKGQTLEQAVDNGKRYVTACLKAMLDLG"
     assembly_gap    1207..1321
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1322..1854)
                     /gene="thiE"
                     /locus_tag="DHV81_08875"
     CDS             complement(<1322..1854)
                     /gene="thiE"
                     /locus_tag="DHV81_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCI94543.1"
                     /translation="MNKTDLKLYAITDRQWLHKARLSEHVKLAIEGGATMIQIRDKDI
                     LSTDSDAGLKDEYNEALEIKRICHEHKVPLIINDNVQFAIDIDADGVHLGQDDMNPAE
                     ARKLLGADKIIGVTAKTVEQAKRAEADGADYLGSGAVFGSTTKLNAKPMTKELLREIT
                     AAVDIPVVAIGGINADNA"
     gene            complement(1938..2885)
                     /locus_tag="DHV81_08880"
     CDS             complement(1938..2885)
                     /locus_tag="DHV81_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCI94544.1"
                     /translation="MNNFIEKLKSRSPLIHNITNMVTINDVANIELACGARPIMAMAP
                     QEMDEVTGICDGLNLNIGTPSEERFEAMRIAARMAAKKNIPIVLDLVGVGVSRFRMDF
                     VMSLLDEVHVDVIKGNLSEVKAVMEHGRCDGGVEVTDTADESDAKALACRAALRYGCI
                     CVITGETDYVAELCDEADCYDNNESSQMSTDATGTGVMDNCVVNAVALDADGYTHNYR
                     VGSITGGHPMMKRVTGTGCMLSGLICAFVAADCDDKYGAVTAALSCMKSAGGLAASDM
                     SEHGRGTNSCYFIKPGNAAYRDRLIDAVYHICDGDYELM"
     regulatory      complement(2962..3071)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3669..4430)
                     /locus_tag="DHV81_08885"
     CDS             complement(3669..4430)
                     /locus_tag="DHV81_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCI94545.1"
                     /translation="MTKVIMNGCNGKMGQTITEICKADADIKIVAGIDLYDGIDNDYP
                     VFSSIDKCDVDADVVIDFSNAKAVDGVLDYCVDKQVPVVLCTTGLSDEQLAKVEECYK
                     KVAVLKSANMSLGINTLMALLKKAAQVFAPAGFDMEIVEKHHNLKLDAPSGTALALAD
                     SINEAMDNEYHYVYDRSQRREKRDPKEIGISAVRGGSIVGEHEVLFCGQDEVIEFKHT
                     AYSKAVFAKGAVEAAKFLKGKGAGHYDMADVIAAK"
     gene            complement(4455..5345)
                     /locus_tag="DHV81_08890"
     CDS             complement(4455..5345)
                     /locus_tag="DHV81_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCI94546.1"
                     /translation="MAIFKGAGVAIVTPMYDNEEVNYDKLEELINMQIDNGTDAIIIA
                     GTTGESPCLSMEEHAKVIKAAVEFTKHRVPVVAGTGSNCTKTAMQLSKEAEEFGADGL
                     LVVTPYYNKATQAGLVSHYSQIADSTKCPIIVYNVPGRTGCNVLPETMAELFKTKENI
                     VGLKEATGNLAQASKTMYLTDGKLDMYSGEDGLVVPLMSIGAIGVISVWSNVAPAKVH
                     NMCDSFFKGDLQTALELQREALPLVDALFSEVNPIPVKKAMNLMGMNVGPLRSPMCEM
                     GEDNARKLAEVMKAYGLKLA"
     gene            complement(5741..6220)
                     /locus_tag="DHV81_08895"
     CDS             complement(5741..6220)
                     /locus_tag="DHV81_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--corrinoid adenosyltransferase"
                     /protein_id="HCI94547.1"
                     /translation="MENGAVQIYYGEGHGKTNVAIGSAICAACDGKQAVVIQFLKGKK
                     EPEFLKKLEPEVKFFNFSRAGILFEQLDEESKQEEIMNIKNGFNYAKKVISTGADMVV
                     VLDEMLGLLDLHLISKEELSDMLASRPDDVTVIMTGRVLDDGIREMADEIYNIASEK"
     gene            complement(6404..>6722)
                     /locus_tag="DHV81_08900"
     CDS             complement(6404..>6722)
                     /locus_tag="DHV81_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743359.1"
                     /note="binds to single stranded DNA and may facilitate the
                     binding and interaction of other proteins to DNA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCI94548.1"
                     /translation="SDYDEDAANQIFLDGYICKEAIYRKTPLGREIADLLVAVNRSYG
                     KSDYIPCICWGRNARFASTFEVGTHVQIWGRIQSRDYVKKLSETQVEQRTAYEVSVSK
                     IEA"
     assembly_gap    6723..6847
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6848..7062)
                     /locus_tag="DHV81_08905"
     CDS             complement(<6848..7062)
                     /locus_tag="DHV81_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743359.1"
                     /note="binds to single stranded DNA and may facilitate the
                     binding and interaction of other proteins to DNA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCI94549.1"
                     /translation="MADKMFENNQVSIIGEIVSDFQFSHEVYGEGFYMMEVSVRRLSD
                     CMDYIPVMVSERLINVEGDYIGKSVYIG"
     gene            7273..>7684
                     /locus_tag="DHV81_08910"
     CDS             7273..>7684
                     /locus_tag="DHV81_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCI94550.1"
                     /translation="MKTTRDDIRNIAIIAHVDHGKTTLVDELLKQSGVFRDNQEVQDR
                     VMDSNDIERERGITILSKNTAIQYKDIKINIIDTPGHADFGGEVERVLKMVNGVILVV
                     DAFEGVMPQTKFVVMKALALKLPIIVFVNKIDRPE"
BASE COUNT         1932 a         1989 c         1330 g         2124 t
ORIGIN      
        1 ctcatgacct tctgcatctc cacatcatag tccttgcagt ctcttgacac accgaggttt
       61 acattgattt ttgtgcgaag catgcttcca acaccctctg catcaagtga cttgtgatgc
      121 ttgttagcag gaatagctac ctttccgctt gcaacaagct ccatcagctt gtctacatcc
      181 atcttctctt tctttgctac cttttcaatt tcaggtgtta cgatgccctt tcttgcggca
      241 tccatctgtg ttgtgtaatt actcattgta ctctcctgtt cttnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccagatc
      361 aagcatggct tttaaacatg ccgtgacata tcgcttgccg ttatccacag cctgctcgag
      421 cgtctgtccc tttgccaggc cgctggctat cgctgatgaa agcgtgcagc ctgtgccgtg
      481 tgagtttgga ttatccactc ttttggtctc aaatgtgatg atttgctttc catcccacaa
      541 atagtcacac acacatgctg tgtctgtgaa atgacctccc ttgcttaaaa ccggacagcc
      601 gtatttatca taaatggcct ttgcagcctc tatgatgtca tctgtgcttt caatctgtct
      661 gcctgatatc acctcaagct cagggatatt tggcgtaatg atgtcagcca gtggaaacag
      721 cttctccatc agagtgtttg cagcattttc ttcaagcagc ctgcttccgc ttgtagcgac
      781 catcaccggg tccagaacta tgttttttgc attatatttt acaagtcctg cggcaatcgc
      841 ctcaattgta tctaccgatg atgtcatacc gattttcacc gcatccggaa aaatatccgt
      901 aaaaacagcc ttcatctgtg cctcgataaa atccgccggc acattcatca ccgcgtcaac
      961 tcccatggtg ttttgtgccg tgagtgccgt gataacgctc tcgccaaaca caccgtttgc
     1021 aagcatggtc tttaaatcag cctgcacacc tgcgccaccg cttgaatcgc ttcccgcaat
     1081 tgtaagtgct gtatgcatat ttacgctcct ttctactgaa tgttgtgatt tctcgtaact
     1141 gttcagtatc ttcaaaatat tgtttaatta ctgtagttat agcagtatgt catactgtga
     1201 ttacagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 ngcattgtct gcatttatgc ctccgattgc aacaaccggt atatcgacag ccgccgtgat
     1381 ttcacgcaga agttcctttg tcataggctt tgcattgagc tttgtggtag agccaaacac
     1441 ggctccgctt cccagatagt ccgcaccatc agcttctgcc ctctttgcct gctccacggt
     1501 ctttgccgtg actcctatta tcttgtcagc tccaagaagc ttccttgcct ccgccggatt
     1561 catgtcatcc tgtcccagat gtactccgtc ggcatctata tcaattgcaa actgcacgtt
     1621 atcattgatg atgagcggta ccttgtgctc atgacagatt ctctttattt caagagcctc
     1681 attatactca tccttaagcc ctgcatcaga atccgtactc agaatatcct tgtcgcggat
     1741 ctgaatcata gtggcaccgc cctctattgc aagcttcaca tgctcagaaa gcctcgcttt
     1801 gtgcagccac tgcctgtctg ttatagcgta gagctttaaa tcagttttgt tcatatatac
     1861 ctttcacctc ttatcctgtt gttttacaaa tataagactc atgacctgca taccaattgt
     1921 ctgtcataaa gcgatattca catcagttca taatcaccat cgcatatatg atatactgca
     1981 tcaatcagcc tgtcccggta tgcagcattt ccgggcttaa tgaaataaca gctgtttgtt
     2041 cctctgccgt gctctgacat atcagaggca gcaagcccgc ctgcactctt catgcagcta
     2101 agtgctgctg tgactgcacc gtatttatca tcgcaatcag ccgcaacaaa cgcacaaatg
     2161 agccctgaaa gcatacatcc ggtgcctgtc acccgcttca tcataggatg gccgcctgtg
     2221 atacttccta ccctgtaatt atgcgtgtat ccgtcagcat ccaacgcaac agcattaact
     2281 acacaattat ccatcacacc ggtacccgtc gcatcagtac tcatctgact actttcatta
     2341 ttatcatagc aatcagcttc atcacataat tctgccacat aatcggtctc acctgtaatc
     2401 acacagatgc agccatatcg cagggccgcc ctgcacgcaa gcgcctttgc atcagactca
     2461 tctgccgtat cggtcacttc cacgccgcca tcacatctgc catgttccat aacagccttg
     2521 acctcagaca aattgccctt aatcacatcc acatgcacct cgtcaagcag gctcatcaca
     2581 aaatccatcc tgaagcggct cacgcccaca ccaaccagat caagcacaat aggaatgttc
     2641 tttttcgctg ccatcctcgc agcaatgcgc atcgcctcaa agcgctcctc agagggagtg
     2701 cctatattca gattcagccc atcgcagata cccgtgacct catccatctc ctgcggtgcc
     2761 atagccataa taggcctggc cccacatgcc agctcaatat tggccacatc attgatagta
     2821 accatatttg tgatgttgtg aatcaaagga ctgcgtgact taagcttttc tataaaatta
     2881 ttcatctgta tctctttaca actattcata atcacccaca cattcaaaaa gcggatatac
     2941 cgccaatgag tatatccgcc taaaaatcat gcgatattat cctacgttgg cattatccaa
     3001 atcaggttat gggtcaagac actaccagtc tactctcagc cggctaaata ccagctcccc
     3061 tgttgtgttt taaaattaaa tccaataaca atgtaactat ccaaaacaag aaaatctgct
     3121 gcttacatgc aagcatgacg catcagcttt tttgttctca gtggttctga atagtcatta
     3181 tatttaatat aatacgcact ccccaataaa tcaagtaaaa tccgcaagtg tacccatatt
     3241 cgtacatcaa gcgctgcttg tcacactgtt aggtgtgaac agtgaacagt aaccatcaaa
     3301 cacaatcccg gtaacaccga gccccgaggt gttaccatag cactcattat cccgccacaa
     3361 cgtaagggcg taagccccgg gcaccggggt gcacacgcac tgtcccgaag cgcgaatagc
     3421 gaagtggctt agatactgta gaaattatgc ccatgaagtg cagaaacacc cctgcatgga
     3481 cgcaggggtg aggagaaccg cgccacggac ggcgcgtttc gacggtttcg tcaagcatag
     3541 aaacccatca ggcataattt cgtacagaat cttagccacg tagcgtagcg ccccgggaga
     3601 gtgcgtgtgc accccggtgc ccgggtccta cgcccgacct attgccacaa tgaagcaatt
     3661 cacctaatct acttagctgc aataacatcc gccatatcgt agtgacctgc accctttccc
     3721 ttaaggaact tggcagcctc aacagcaccc ttcgcaaaaa cagccttaga gtaagcagta
     3781 tgcttaaact caatcacctc atcctgtcca cagaaaagta cctcgtgctc accgacgatg
     3841 cttcctcctc tgacagcaga aataccaatt tccttaggat cacgcttctc tctgcgctgg
     3901 ctgcggtcgt aaacatagtg atactcgtta tccatagcct cgttgatgga atcagcaagc
     3961 gcaagcgcag tacctgaagg tgcatcaagc tttaaattgt gatgcttctc aactatctcc
     4021 atgtcaaaac ctgccggtgc gaaaacctgt gcagcctttt tgagaagagc cataagagtg
     4081 tttatgccaa gtgacatgtt tgcagacttg agcactgcca ccttcttgta gcactcctca
     4141 acctttgcaa gctgctcatc actaagaccg gtagtgcata aaacaaccgg aacctgcttg
     4201 tccacgcaat agtccagtac tccgtcaaca gccttggcat tggaaaaatc tatgaccaca
     4261 tcagcgtcca catcacattt gtcgatgctc gaaaaaacag gatagtcatt gtctattcca
     4321 tcatataaat caataccggc tacaatcttg atatcagcat cagccttaca gatttctgtg
     4381 atagtctgtc ccattttgcc gttgcagcca ttcataatta cctttgtcat tttatcgtat
     4441 cctttctcgt tgtactaggc aagctttaag ccataagcct tcatgacctc agcaagcttc
     4501 cttgcattat cctcacccat ctcacacata gggcttctaa gaggtccgac attcataccc
     4561 atcaggttca tagccttctt tacaggaatt ggatttacct ctgagaagag tgcgtccaca
     4621 agtggaagag cctctctttg gagctctaag gctgtctgta aatcgccctt gaagaagctg
     4681 tcacacatat tgtgtacctt tgcaggggct acattgctcc atactgagat tacaccgatt
     4741 gctccgatag acataagagg cactacgaga ccatcctcgc ctgagtacat atcaagcttt
     4801 ccgtctgtca ggtacattgt ctttgaagcc tgagccagat ttcctgtagc ttccttgagt
     4861 cccacgatat tttcctttgt cttaaagagc tctgccatag tctccggaag cacgttgcag
     4921 cctgtacgtc ccggtacgtt gtatacgata ataggacact ttgtgctgtc tgcaatctgc
     4981 gaatagtggc tcacaagacc tgcctgagta gccttgttgt agtatggtgt gacaacgagc
     5041 agtccgtctg caccaaactc ctcagcctcc tttgaaagct gcatagctgt ctttgtgcag
     5101 ttagagcctg tgcctgctac tacaggtaca cgatgctttg taaactctac ggcagccttt
     5161 ataacctttg catgctcctc cattgacaga catgggctct cgcctgtggt gccggcaatg
     5221 ataatagcat ctgtgccatt atcaatctgc atattgataa gctcctcgag cttgtcgtaa
     5281 ttaacctcct cgttatcgta cataggtgta acaattgcta ctcctgctcc tttgaaaatt
     5341 gccatggttt tatctccttc ttgcttttct ttattcggta tttgattctc ttaacgtata
     5401 agtatgccgc cgtctgcctc taagcgctaa aaaaccggct ccttagagcc ggtcccaatt
     5461 cttcatgaat cacgatagct ccccatcaaa tctgatgaca gtcataaggc tgttcgccat
     5521 atgcccagag atatatccgc agatgataaa tcccttcggc gctattcctt tcatctccca
     5581 tcaactgccc ctgcggcatc cggaaagcta ctcataagtc gcgcgacctc tatctgttaa
     5641 tcaaattatt cgtatacagc agatgatata actgcctcac acttgctcta ataatatcac
     5701 cacgcctgtg cattgtcaac tcacatgaca ataattctta ttatttttcc gacgcgatat
     5761 tataaatctc atctgccatt tccctgattc catcgtcaag tactctgcct gtcataatca
     5821 cggtcacatc atcaggcctt gaagcaagca tatccgacag ctcctccttt gaaatcagat
     5881 gcaaatccaa aagccccagc atctcatcaa gcacaacaac catgtcagca cctgttgata
     5941 taaccttctt tgcatagttg aagccattct tgatattcat gatttcttcc tgcttactct
     6001 cctcatcaag ctgctcaaag agtattccgg ctcttgaaaa attaaagaat ttcacctccg
     6061 gctcaagctt tttcaaaaat tccggctcct ttttaccctt tagaaactga ataacgacag
     6121 cctgtttacc atcacaggca gcacatattg cacttcctat agcgacattt gtcttgccat
     6181 gtccctcgcc ataataaatc tgtactgcac cgttttccat aataatcatg cctccataca
     6241 catagctttt ctgcctgata tacaatcatt acagtttaca ccttatgtaa acgtcaacaa
     6301 ttacaagcat taaccaataa tatctagtaa aataccacaa tctgactttt attgcaaatt
     6361 ttcgggaaaa tcacaaatcc gtagttactg tccattaaaa atactatgcc tcaatcttgc
     6421 tcacggaaac ctcatacgcc gtgcgctgct ccacctgtgt ctcactgagc tttttcacat
     6481 agtctctgct ctggattctg ccccatatct gcacatgtgt acccacctca aatgtcgacg
     6541 caaatctggc atttcttccc caacagatac atggaatata gtctgacttg ccatatgagc
     6601 ggttcactgc cacaagcagg tctgcaattt ctcgtccaag cggtgttttc ctgtatatag
     6661 cctccttgca gatatagccg tcaaggaaaa tctgattggc tgcatcctca tcataatcac
     6721 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnncct atatatacgc ttttgcctat gtagtcaccc tccacattga taagcctctc
     6901 tgacaccatc accggaatat agtccatgca atctgaaagc cttctgaccg aaacctccat
     6961 catatagaag ccctcaccat acacctcgtg actaaactga aaatctgaaa cgatttctcc
     7021 gattattgat acctggttgt tttcaaacat tttatctgcc atgaattttt gtctcccttc
     7081 cttttgtaaa atgtttttcc agctattttt aacatttcca caaagtatat tttctcaaat
     7141 aatgtcgaca aatccaataa catttcactt aattttataa aaactttcaa tgaaaacttg
     7201 cacattggaa aaattatgat agaataagtt agttgcaaac ttagctttat ttattaaaga
     7261 aagaaggaaa atatgaaaac aacaagagac gatattcgta atatagcaat tattgcccac
     7321 gttgatcatg gtaaaacaac acttgtagat gagcttctta agcaaagcgg tgtgttccgt
     7381 gacaatcagg aagttcagga tcgagtaatg gattcaaacg acatcgagcg tgaaagaggt
     7441 atcacaatcc tctccaaaaa cacagctatt cagtataagg acatcaaaat caacatcatc
     7501 gacactccgg gtcatgctga cttcggcggt gaggtagagc gtgttcttaa gatggtaaac
     7561 ggagttatcc tcgtggttga tgccttcgag ggtgttatgc cacagaccaa gttcgtagtt
     7621 atgaaggctc ttgcactcaa gctgcctatc attgtctttg tcaacaagat tgaccgtccg
     7681 gagg
//
LOCUS       DPUF01000257            4631 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_25856, whole
            genome shotgun sequence.
ACCESSION   DPUF01000257 DPUF01000000
VERSION     DPUF01000257.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_25856"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            101..904
                     /locus_tag="DHV81_08915"
     CDS             101..904
                     /locus_tag="DHV81_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HCI94551.1"
                     /translation="MYKTKIDNLDLKQIADSGQCFRWKNTGENEYTVVAFDRALRIKQ
                     DGNEFELDCDEADWNNIWKSYLDMDTDYAGIAKLIADGDDAHLKEAYAYGSGVRILRQ
                     DLWEMIVTFMISQNNNIKRITNSVDLLCRRCGHKIDGSAEGEDLYTFPKPLEVPDEVF
                     DDRSMGFGYRAPYLKEIYEYGANNPDWLDNLRKMSYDEAMESLLSRKGIGKKVANCIC
                     LFGLHHVDAFPIDTHVKQLLDKYYSDGFDFERYKGVAGIIQQYLFYFEL"
     gene            933..1493
                     /locus_tag="DHV81_08920"
     CDS             933..1493
                     /locus_tag="DHV81_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="HCI94552.1"
                     /translation="MYVIVGLGNPDKKYEHTRHNIGFDVIDALADKYNISVTDKKHKA
                     LCGSGVIEGMKVLLVKPQTYMNLSGESVAEVMNFYKLDPDEEMIVIFDDISLEPGRIR
                     IRKKGSAGGHNGIKSIIAMTGTQGFSRIKVGVGEKPQGWDLADHVLGRFSSEDRAKVE
                     EAIGHAMDAAVLMMQGETDKAMNIYN"
     gene            1543..4631
                     /gene="mfd"
                     /locus_tag="DHV81_08925"
                     /pseudo
     CDS             1543..4631
                     /gene="mfd"
                     /locus_tag="DHV81_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741366.1"
                     /note="too many ambiguous residues; incomplete; missing
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
     assembly_gap    3450..3522
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4179..4230
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1348 a          800 c         1287 g         1071 t
ORIGIN      
        1 aatatatttc acacaaaccg gtctatccgt ggtacaataa gccacagaaa gaccggcttt
       61 ttagagtata gctacaataa aagaggatag acaaacagat atgtacaaga cgaaaataga
      121 taatctggat ttaaaacaga ttgctgacag tggtcagtgc ttcagatgga agaatacagg
      181 agaaaatgaa tacactgtag tggcatttga cagggcgctt cggattaagc aggatgggaa
      241 tgagtttgag ctggattgtg atgaggcaga ctggaacaat atatggaaaa gctatctgga
      301 tatggatacg gattatgcgg ggattgcaaa gcttatagca gacggagatg acgcgcatct
      361 gaaggaggcg tatgcatatg gcagtggtgt gcgcatttta aggcaggatt tgtgggagat
      421 gattgttact tttatgattt cccagaataa taacataaag cgaatcacaa acagcgtgga
      481 tttactttgc aggcggtgcg gacataaaat agacggcagt gcagaggggg aagatctgta
      541 tacatttccc aagcctcttg aggtaccgga tgaggtattt gatgacagga gcatgggctt
      601 tggctacagg gcaccgtatc tgaaggaaat atatgagtat ggtgctaata atcctgactg
      661 gcttgataat ctgcgcaaaa tgagctatga tgaggcgatg gaaagcctgc tttctcgcaa
      721 aggaattggc aaaaaggtgg cgaactgcat atgtcttttt ggtttgcatc atgtggatgc
      781 gtttccgata gatacgcatg taaagcagct tttggacaaa tattatagtg acggctttga
      841 ttttgaacgc tacaagggtg tggcgggaat tatccagcag tatttgtttt attttgaatt
      901 gtaaatgata tgagaaagag aatggataaa cgatgtacgt tatagtagga cttggaaatc
      961 ctgacaaaaa atatgagcac acaagacaca atatcggatt tgatgtgata gatgcgcttg
     1021 cggataaata taatataagt gtgacagaca aaaagcacaa ggcgctctgt ggcagtggag
     1081 tgattgaggg catgaaggtg ctgctcgtga agccacagac atacatgaac ttaagcggtg
     1141 agtctgttgc tgaggtgatg aatttttata agcttgatcc ggatgaggaa atgattgtta
     1201 tttttgatga tatttcgctt gagccggggc gtatccgtat ccgcaaaaag ggcagcgccg
     1261 gcggtcacaa cgggattaag agcatcattg caatgaccgg cacacagggc ttcagccgta
     1321 tcaaggtcgg tgttggagag aagccgcagg gctgggatct tgctgaccat gtgctcgggc
     1381 gtttcagttc tgaggacagg gcaaaggtgg aggaagctat cggtcatgcg atggatgcag
     1441 cagttcttat gatgcagggc gagacggata aggctatgaa catatataac tagctggaaa
     1501 tcgacaaggt gattttgaac agacagaaat ggagaattga aagtgaaagc atttacagag
     1561 ccactccttt cccttgcggg ctttgaggag atgacaaaaa cggcagaaaa atcgtccgga
     1621 cttatatctg tcacgggatg tatagatgcg caaaaatcac agatgatata tgcctttgga
     1681 ggccacagga aaaacaagct tattgtgacc tttggagagc agaaggcgaa ggaactgtac
     1741 gatgagtaca gtttttttga caaagaggtg gtgtattatc catcaaagga tgtgcttttt
     1801 taccagtcgg atatcagggg caatctcctg acagcagagc gtatcagggc tttgaaggct
     1861 atccgtgagc aggagagagt gacgcttgtg accacctttg atgcgctgat gaatacgatg
     1921 gcgcctatag aaaagatgtg ggaaaacgtg cttacacttg agcagggaca gcttttggat
     1981 ttgaaggaaa ttcaggctgc tcttatcaga atgggctatg aaaaagagta tcaggtccag
     2041 actatgggac agttttctgt caggggcggt attcttgatg tttttccact gacggaggag
     2101 aatcctatcc gaattgagct gtggggagac gagatagaca ccatcagata ttttgactgc
     2161 gagagccaga aatccattga aaatattgat cgggtgagca tatatccggc ggcagagctt
     2221 gtacttagcg atgaggaaaa ggccggggga attgacaagc ttaaggcaga ggcaaagcgt
     2281 gtgtctgaca agcttagaaa gcagatgaag acagaggaag cacacagagt gactgtgatg
     2341 gcagacgagc ttaccgagga atggggagag ctgtcaatgt atgcgggcat ggatgctttt
     2401 ttgtcgtact tttttgacga gagagtcggg cttttggatt attttaagcc atcggacagc
     2461 cttatatttt tcgatgagct gaccagatgt acagagcagg gaaagctcac agagactgag
     2521 ttttcagaga gcatgaagca gagacttgcg atgggatata tacttccggg gcagatgaac
     2581 ggtcttttta cagaaaagga gattgtggca aagctcgaga agtattcgtg tatcgcactc
     2641 gctgcgctcg ataataaagc aaacggactg caccagctgg gaagctatgg tattcactgt
     2701 cagagcgtca gcccgtacaa caacagcttt gagcttttga taaaggactt aaagcgctat
     2761 aagaaaaacg gctacagagt cattctttta tccggttcaa ggacgagggc aaagaggctt
     2821 tcagaggata tcacagatca gggaatcaca tgcttttata ctgaaaacta cgaccatgag
     2881 ctttcacagg ggcagattat ggtctgctac ggcaaggtga gaagaggctt tgagtatcct
     2941 atcctaaagt ttgccgtgat tacggagtca gatatttttg gggcgcagca gaagaaaaag
     3001 aagcgtcacc gcacctatga gggtgaaaaa atacagagct ttaccgattt gtcggttggg
     3061 gactatgtag tgcatgaaaa ccacggacta ggtatttacc gcggcattga aaagcttgag
     3121 gtggacaaaa aggtaaagga ctacatcaag atagagtatc agggtggctc caatctctat
     3181 attcttgcca cccagcttga catgatacaa aaatatgccg gaaaggatgc cagaaaacca
     3241 aggctcaaca agcttggagg tcaggaatgg accagaacaa agaatagagt cagaggtgcg
     3301 gtaaagcaga ttgcgggaga cctggtgaag ctgtacgcac agcgtgagca gcaaaacgga
     3361 tatgccttcg gcgaggatac ggtatggcag agagagtttg aggagctttt cccttttgat
     3421 gagactgagg atcagattct tgcaattgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgggcta cggcaagacg
     3541 gaggttgcaa tccgtgcggc cttcaaggcg gtgcaggaca gcaagcaggt ggcatatctt
     3601 gccccgacaa ccattctggc acagcagatt tacgatacgt tttcgcagag gatgaaggat
     3661 tttccggtga atgtggattt gatgtgtcgt ttcagaagtc cggcggagca gaaaaagacg
     3721 atagagaagc ttaaaaaggg tgaggtcgat atcattatcg gaacacaccg catactctca
     3781 aaggatgtgc agtttaagga tctgggactt ttggtaatcg atgaggagca gcgcttcgga
     3841 gtgactcaca aggagaagat taagcagatg aaggtcaatg tcgatgtgct cacactgacc
     3901 gctacaccga ttccgaggac actccacatg agtttaatcg gcataaggga tatgagcgtg
     3961 cttgaggagc cgccgatgga ccgtgtaccg attcagacat atgtcatgga gtacaatgat
     4021 gagcttgtca gagaggcgat aaacagggag cttgccagaa acggacaggt gtactatgtg
     4081 tacaataagg tgcgcgacat agcagacata acagcaaagc tgcaggagct ggtgccggat
     4141 gcgactgttg catttgcgca cggacagatg aaggagacnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgcttgtat ccacgaccat tatagagaca
     4261 ggacttgaca tctcaaacgt caatacaatg attatccacg atgcggacaa catgggactt
     4321 tcacagcttt atcagcttcg tggacgagtc ggcaggtcta atcgtactgc gtatgctttc
     4381 ctcatgtaca ggcgcaacaa gatgttaaaa gaggtggcag aaaagcgtct tgcagcgata
     4441 aaggagtact cagaccttgg cagcggcttt aagattgcca tgagagacct tgaaatccgt
     4501 ggtgcgggca atctgctcgg tgcggagcag agcggacaca tggaggcagt gggctacgat
     4561 ttgtactgca agatgttgag cgaggctgta aaagaggcaa agggcatcga ggatatcagt
     4621 gataaatttg a
//
LOCUS       DPUF01000258            6792 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12711, whole
            genome shotgun sequence.
ACCESSION   DPUF01000258 DPUF01000000
VERSION     DPUF01000258.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12711"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="DHV81_08930"
     CDS             <1..95
                     /locus_tag="DHV81_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCI94553.1"
                     /translation="VYKYKRKTGYHKKNGHRQAFTQVKIEKING"
     gene            95..424
                     /locus_tag="DHV81_08935"
     CDS             95..424
                     /locus_tag="DHV81_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCI94554.1"
                     /translation="MIAITVYQNSKGHKIGFKSKGHAGFADSGYDIICAGVSALVINF
                     INSAEELCHAEYSLDTDEETGMIDYRLDKPATGDVALLMDSMILGLKGIQRDYGNEYI
                     ILDFKEV"
     gene            428..>705
                     /locus_tag="DHV81_08940"
     CDS             428..>705
                     /locus_tag="DHV81_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007889948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCI94555.1"
                     /translation="MLNMNLQFFAHKKGVGSTKNGRDSESKRLGAKRADGQFVKAGNI
                     LYRQRGTKIHPGTNVGIGGDDTLYAKVDGVLRFERVGRDKKQASVYPVV"
     assembly_gap    706..819
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            871..2154
                     /locus_tag="DHV81_08945"
     CDS             871..2154
                     /locus_tag="DHV81_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCI94556.1"
                     /translation="MFADRAKIIIKSGKGGDGHVSFRREKYVPNGGPDGGDGGKGGDV
                     IFLVDKGINTLTDYRHRRKFAAEPGQEGGKKNCHGKNGEDLILKVPEGTLIKDAASGK
                     VIADMSGDNTRQVILRGGKGGQGNQHYATSTMQAPKYAQPGQDAIEIEVQLELKVIAD
                     VGLVGFPNVGKSTLLSRVTNAQPKIANYHFTTLQPNLGVVDMDEGFGFVIADIPGLIE
                     GASEGIGLGHEFLRHIERTKVMIHMVDAAGTEGRDPVADIKAINKELEAYNPQLLKKP
                     QVIAANKIDAIAGDENEVISALRAEFEPQGIKVFPISAVSGKGLKELLYEVKNLLANC
                     PKEVTVYEPEIDPALSFFKDEPYAVEVAADGAYEISGPKIEKMLGYTNMDSEKGFAFF
                     QRFLREQGIIKELENMGIEDGDTVRMYDFEFDYYK"
     gene            2169..2465
                     /gene="yhbY"
                     /locus_tag="DHV81_08950"
     CDS             2169..2465
                     /gene="yhbY"
                     /locus_tag="DHV81_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCI94557.1"
                     /translation="MNLSSKQRAYLSSLASTINPIFNIGKASLTPEIIEALDAALEKR
                     ELIKVAVLKNCIDDPREIASVIGERTHSAVVKVIGKKMIFYRPAKKNPKIKLPE"
     gene            2469..3095
                     /gene="nadD"
                     /locus_tag="DHV81_08955"
     CDS             2469..3095
                     /gene="nadD"
                     /locus_tag="DHV81_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCI94558.1"
                     /translation="MKTGIFGGAFDPIHKGHIYMAQKAMEEYSLDRILLVPSGHSPNK
                     TENAMTAFSHRYNMCKLASEAVPGIEVSDIEIKDESTSYTYVTLQKLKALYPEDELYF
                     IMGGDSLDYFESWMRPDVIARTAIILVMVRENFPKLQMEEKIAHIKNLFPADIRLLKC
                     DRMDVSSTQVRKLLRAKSQADGYRSPKGAEMFLDAAVMSYIQANNLYR"
     gene            3102..3683
                     /locus_tag="DHV81_08960"
     CDS             3102..3683
                     /locus_tag="DHV81_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCI94559.1"
                     /translation="MEVKEIKKKLKKSLDKQRYQHTLGVMYTAGCLAMANGYPIEKAM
                     LAGLLHDCAKCLSTEKKIELCVKKNIDITEVEISNPGLLHAKAGMVLAEEEYGIKDAQ
                     ILHAIRVHTTGEADMGLLDKILFVADYIEPNRCEAPRLEEIRKLAFSDLDRTVAEILY
                     DTINFLNTKSGAIDPTTQITYNYYAKYRDNREE"
     gene            3689..4036
                     /gene="rsfS"
                     /locus_tag="DHV81_08965"
     CDS             3689..4036
                     /gene="rsfS"
                     /locus_tag="DHV81_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCI94560.1"
                     /translation="MQTIDLVKKIVEALEDKKAEDITVLDIGEVSSIADYFIIANGNN
                     ANQLTAMEDAVDEAMYTNGVHQKQVEGTGNSTWILMDYQDIIVHLFSKEDRQFYDLDR
                     IWRDGKVVDVHSL"
     gene            complement(4148..4762)
                     /locus_tag="DHV81_08970"
     CDS             complement(4148..4762)
                     /locus_tag="DHV81_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCI94561.1"
                     /translation="MALEKISDKQKEILEFIKSEILNRGYPPSVRDICEGVHLKSTSS
                     VHAHLETLERKGYIRRDPAKNRAIEIIDDSFGLQRREMVNVPLVGRVAAGEPILAVEN
                     IETYFPVPAEYMPNKQSFMLKVKGESMINAGIFDGDNILVEQQSDASNGDMVVALIDD
                     SATVKTFYKENGHIRLQPENDTMDPIIVPDCEILGKVFGVFRFF"
     gene            5007..5330
                     /locus_tag="DHV81_08975"
     CDS             5007..5330
                     /locus_tag="DHV81_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCI94562.1"
                     /translation="MRGNTLSGRRKMFGWTAVAFIAAVIILCGTIHIFASSGDSRPYN
                     KYYTSVRVEEGDTVWSIADRYIADSQVSKRAYVDEICRLNSLTDGNVRSGDYIVVSYY
                     SQDIK"
     gene            5441..6157
                     /locus_tag="DHV81_08980"
     CDS             5441..6157
                     /locus_tag="DHV81_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCI94563.1"
                     /translation="MLKLFFVIFANFHRAPYIVPKMRYMADHPQKYSEAERYDMMRHC
                     IDLMNKSGKITTEAYGLENLPETGGYIMYPNHQGKYDLLGIITTHDKVLSFVMDKQKS
                     HTMLVREFVDLVQAKRLEKDNPRQGLTIINEVAKDVKNGKRYVLFPEGGYKFNNKNKV
                     QDFKAGSFKIALKSHVPIIPIALIDSYKVFNSFHFGPVTTQVHYLKPIEYDEYKDMKT
                     KDIAALVKERIEEKIKEILG"
     gene            6188..>6792
                     /locus_tag="DHV81_08985"
     CDS             6188..>6792
                     /locus_tag="DHV81_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCI94564.1"
                     /translation="MKDKAYYEDVNIKNELKLRDMIQKLPKFCMDFFIGIDADKSSRT
                     KIAYAYDLQTFFEYMKSANPSLKKYDIRDYPISLLDQISPSDIEEYLFYLKYYEKDGV
                     VHTNDERGIKRKLASLRTFYNFYFRKEFIDTNPASKVTLPKIHEKNIIRLDTDEVAQL
                     LDEVESGDSLSKNQQRFHEKTKVRDLALMTLLLGTGIRVSE"
BASE COUNT         2138 a         1174 c         1579 g         1787 t
ORIGIN      
        1 tcgtttacaa gtacaagaga aaaaccggat atcacaaaaa gaacggacac agacaggctt
       61 tcacacaggt taagattgaa aaaattaacg gataatgata gctataaccg tttatcagaa
      121 ttcaaaagga cataagatag gctttaagtc aaagggacat gcaggttttg cagattccgg
      181 ttatgatata atctgtgccg gggtttcagc gctggtgatt aacttcatca attccgctga
      241 agagctgtgt catgctgagt acagtcttga tacagacgaa gagacaggta tgattgacta
      301 cagacttgac aagccggcga caggtgatgt tgcactgctt atggattcca tgattctggg
      361 actaaaagga atacaaagag actatgggaa tgagtacatc attctagact tcaaggaggt
      421 gtaagacatg ttgaatatga accttcagtt ttttgctcat aaaaagggag ttggttctac
      481 aaagaacgga cgtgactctg agtctaagag attaggcgct aagagagcag acggacaatt
      541 cgtaaaagct ggtaatattc tttacagaca gcgtggtaca aagattcacc ctggtacaaa
      601 cgttggtatc ggtggagatg atacattata tgctaaggtt gatggcgtat taagatttga
      661 gagagttgga agagataaga aacaggcttc cgtatatcca gtagtnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna taaagtatac tgagtgtttt
      841 atgcagacat tcagcatgag gtggaaaatt atgtttgcag atcgtgcaaa aattatcata
      901 aaatcaggaa aaggcggaga tggtcatgta tcattccgcc gtgaaaaata cgtgccgaac
      961 ggtggtccgg acggcggtga cggtggaaag ggcggagacg ttatattcct cgtggataag
     1021 ggaatcaaca ccctgacaga ttatcgtcac agaagaaagt ttgcagcaga gcccggacag
     1081 gagggtggca aaaagaactg ccacggaaag aatggtgagg atcttatact taaggtgccg
     1141 gaaggtacac tcataaagga tgcagcaagc ggaaaggtca tagcagatat gtccggtgac
     1201 aatacaaggc aggtgatcct acgtggagga aaaggcggtc agggcaacca gcactatgcc
     1261 acatccacca tgcaggctcc taaatatgca cagccgggac aggatgcaat agaaattgag
     1321 gtacagcttg agctaaaggt aatagcggat gtagggcttg taggctttcc aaacgtagga
     1381 aaatcaacac ttctgtcacg agttaccaat gcacagccaa agattgccaa ctatcatttc
     1441 accacattac agccaaatct tggtgtggtt gatatggatg agggctttgg tttcgtaatc
     1501 gctgatatcc cgggacttat cgagggagca tcagagggca taggactcgg tcatgagttt
     1561 ttgcgtcata tagagcgtac caaggtcatg atacacatgg ttgatgcggc cggtactgaa
     1621 ggccgtgatc cggttgctga catcaaggcg ataaacaagg agcttgaagc atacaatcca
     1681 cagcttctca aaaagccaca ggtcattgca gccaacaaaa tagatgccat agcaggagac
     1741 gagaatgagg taatttcagc cttaagagcc gaatttgagc cacagggcat aaaggtattc
     1801 ccgatatccg cagtaagcgg taagggcctt aaggagcttt tgtacgaggt gaagaacctg
     1861 cttgcaaatt gtcctaaaga ggttactgta tatgagcctg agattgatcc tgcactttca
     1921 ttctttaagg atgagccgta cgcggttgag gtggcagccg atggtgcata tgagatatca
     1981 ggtccaaaga ttgagaaaat gctcggatat acaaatatgg actcggagaa gggctttgcc
     2041 ttcttccaga gattcttgcg tgagcagggt atcatcaagg agcttgaaaa tatgggcatc
     2101 gaggatggag atactgtccg catgtatgat ttcgagtttg attattacaa gtaaggagaa
     2161 gagtatatat gaatttatca agtaaacaac gtgcatattt aagcagcctt gcaagcacaa
     2221 ttaaccctat tttcaatatt ggaaaggcat cgcttactcc ggaaatcatc gaggctcttg
     2281 atgcagcact tgaaaagagg gagctgatca aggttgcagt tcttaaaaac tgcatagatg
     2341 atccaagaga gattgcaagt gtaatcggtg agagaacaca ctcagcagtg gttaaggtaa
     2401 taggcaaaaa aatgattttt taccgtcctg cgaagaaaaa tccaaagatt aagcttccgg
     2461 aataagccat gaaaacaggg atttttggtg gggcatttga cccgatacac aaaggtcata
     2521 tatacatggc acaaaaggcg atggaagagt attctcttga caggattctg cttgttccgt
     2581 caggtcactc gccgaacaag actgagaatg caatgacagc atttagccac cggtacaata
     2641 tgtgtaagct ggcatcagaa gcagtgcccg gtattgaggt gtctgatatt gagataaagg
     2701 atgagtccac cagctatacg tatgttacac tccaaaagct aaaggcgctt tatccggagg
     2761 atgaactgta tttcatcatg ggtggagatt ctctggacta ttttgagtca tggatgagac
     2821 ctgatgtcat agcccggaca gcaattattc ttgttatggt gagagaaaat tttccaaagc
     2881 tgcagatgga ggaaaagatt gctcatatca aaaatctgtt tcctgcagat ataagacttt
     2941 taaagtgtga caggatggat gtgtcgtcta cccaggtcag aaaactgcta cgtgccaagt
     3001 cgcaggcaga tgggtatagg agtcctaaag gagcggaaat gtttctggat gctgcagtta
     3061 tgtcatatat acaggctaat aatttataca ggtgatagag tatggaagta aaagaaataa
     3121 agaaaaagct aaagaagagc cttgataaac aaaggtatca gcatacgctc ggagtgatgt
     3181 atactgccgg atgtcttgcc atggcaaacg gctatccaat agaaaaggca atgcttgcag
     3241 gcctgcttca tgactgtgcc aagtgtcttt cgactgaaaa gaaaatcgaa ctctgtgtaa
     3301 agaaaaatat agacataaca gaggtagaga tatcaaaccc gggtctgtta catgcaaagg
     3361 caggcatggt gctggctgag gaagagtatg gcattaagga tgcgcagata ctgcatgcca
     3421 taagagtgca tactacagga gaagcagata tggggctcct tgacaaaata ctctttgtgg
     3481 cagattatat tgagccaaac agatgtgaag caccgaggct tgaagagata agaaagcttg
     3541 ctttttcaga ccttgatagg actgttgcag agatattgta tgatacaatc aattttctta
     3601 acacaaagag tggggcaata gatccgacta cccagattac ttacaattat tatgctaaat
     3661 acagagacaa cagggaggaa taactgctat gcagacaatc gatttagtta aaaagatagt
     3721 ggaagcactt gaagacaaaa aggcagagga tatcacagtt ctcgacatag gagaggtttc
     3781 ttctatagca gactacttta tcattgcaaa cggaaacaat gcaaatcagc tcacagccat
     3841 ggaggatgct gtggatgagg ctatgtatac aaacggagtg catcagaagc aggtagaggg
     3901 aacaggcaac tctacatgga ttctcatgga ctatcaggat attatcgtgc atctgttctc
     3961 taaagaagac agacagttct atgatctcga cagaatatgg agagatggaa aagtggtgga
     4021 tgtacattca ctgtagtatt tattctatga ttaaggaatt ataaatcgaa accggtaatt
     4081 taaaatatcg cacccatatg ccgagctgtt tagcttagca tatgggtgcg atattttgca
     4141 attaatatta gaagaaacga aatactccaa acactttgcc gagtatttca cagtccggaa
     4201 ctatgattgg gtccattgtg tcgttctccg gctgaagacg tatgtgacca ttctctttgt
     4261 agaaggtctt tacggtagcg gaatcatcta taagtgctac aaccatatct ccgtttgaag
     4321 catcgctctg ctgctcgacg aggatattat caccgtcgaa gataccggca ttaatcatgc
     4381 tctcgccctt gactttaagc ataaatgact gcttatttgg catatattca gcaggaacgg
     4441 gaaaataggt ttcgatattt tccactgcaa gtatcggctc acctgcagct acacgaccga
     4501 ccaaagggac attaaccatc tcgcgacgtt gtaaaccaaa gctgtcatct ataatttcta
     4561 tagccctgtt tttggcagga tctcttctta tgtagccctt tcgctccagt gtctcgagat
     4621 gagcatggac gctggatgtg gactttaaat gtacaccttc gcagatatcg cgcactgatg
     4681 gcggatagcc cctgtttaaa atctcactct tgataaactc cagtatctct ttctgcttgt
     4741 ctgaaatctt ttctaatgcc ataggtaaat cctccgtctg aatattaaaa taatataatg
     4801 atatggggca aaaggaattt tgcgccccat gaatgattgt aaaaataaaa attatatctt
     4861 agaaaaagta tatcataaaa ccaaagcaaa cgcaaacata tattccgaaa ttttgttcgt
     4921 ttttctattg acaaacgatt gttcgagatg tattatgtaa ccacaataac aacaaacaca
     4981 tgttcggaaa tacaagaggt ggttttatga gaggaaatac tttatcaggt agaagaaaaa
     5041 tgtttggatg gactgctgtt gcatttatag cagctgtcat tatattgtgt ggcactattc
     5101 atatttttgc cagttccggt gacagcaggc cttataacaa atattatacc agcgtcaggg
     5161 ttgaggaggg cgacactgtc tggagcattg ccgatagata tattgccgac agtcaggtga
     5221 gcaaaagagc ttatgtagat gaaatttgca ggctcaatag tcttacagat ggcaatgtcc
     5281 gcagtggcga ttatatagtt gtttcatatt attctcagga tataaagtaa cgttttcttg
     5341 ctaaaatttc ctgtttctta tataatatag gttaattgca gcggatttgg gttatagtat
     5401 ctatggctta tccgtttttg aaacaggaga ttggatattt atgttaaaac tattttttgt
     5461 tatatttgca aattttcata gagcgccgta tattgttccc aaaatgagat acatggctga
     5521 tcatccgcag aaatattcag aagcggagag atatgatatg atgcggcact gtatagattt
     5581 aatgaataaa tcgggaaaaa ttacaacaga agcatatggt cttgagaatc ttcctgaaac
     5641 aggtggctat atcatgtatc ccaaccatca gggaaagtat gatttactgg ggattattac
     5701 tacacatgac aaggtactgt cgtttgttat ggataagcag aagtcgcata caatgcttgt
     5761 cagggaattt gtggatctgg ttcaggcaaa gcgtttagag aaagataatc caaggcaggg
     5821 gcttactata atcaatgagg tagctaagga tgtgaaaaac ggcaagagat atgtcttgtt
     5881 tcctgagggt ggctataagt tcaataataa gaataaagtg caggatttta aagcaggtag
     5941 ctttaagatt gcattgaaat cacatgttcc tattattccg atagctctta tagattcata
     6001 taaggtgttc aacagctttc atttcggccc tgttacaacg caggttcact atttaaagcc
     6061 tattgagtat gatgagtaca aggacatgaa gacgaaggat atagctgcac tggtcaagga
     6121 gcgtatagag gagaaaatca aagaaatact tggttagagt caactttgat tattatatag
     6181 tattattatg aaagacaaag cttattacga agacgtaaat ataaaaaatg aattaaagct
     6241 cagagatatg atacagaagc ttccgaagtt ctgtatggat ttttttatcg gaatagatgc
     6301 ggataagtcc tcaagaacca agatagcgta tgcatatgat ctacagacct ttttcgagta
     6361 catgaagagt gccaatccat cgcttaagaa atatgatata agggactacc ctatatctct
     6421 gttagatcag atcagtcctt cagatattga ggaatatctg ttttatctaa agtattatga
     6481 gaaggatggc gtcgtacaca ccaatgacga gagaggcatt aagcgaaagc ttgcatcact
     6541 gcgcactttt tataattttt atttcaggaa agagtttata gataccaatc ctgcgagcaa
     6601 ggtaaccctt cccaaaatac atgagaagaa tatcatccgt cttgatactg atgaggtcgc
     6661 acagcttctc gatgaggttg agtcaggaga cagtctgtcc aagaaccagc agcgtttcca
     6721 tgagaaaaca aaggttcgtg accttgcact tatgacactg cttcttggaa caggtatccg
     6781 tgtatctgag tg
//
LOCUS       DPUF01000259            7739 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_15042, whole
            genome shotgun sequence.
ACCESSION   DPUF01000259 DPUF01000000
VERSION     DPUF01000259.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_15042"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="DHV81_08990"
     CDS             complement(<1..170)
                     /locus_tag="DHV81_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HCI94565.1"
                     /translation="MRGVETRIQEIRHKIFTEVARMAYHTEWPVKDRMEALPYKIIPG
                     EKGNFRNDVFLER"
     gene            488..643
                     /locus_tag="DHV81_08995"
     CDS             488..643
                     /locus_tag="DHV81_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HCI94566.1"
                     /translation="MEVKYYVCNHCGNIIEMVKDKGVPVADFCLCDGEQVEEVYAYCN
                     LHGLWKC"
     gene            637..1860
                     /locus_tag="DHV81_09000"
     CDS             637..1860
                     /locus_tag="DHV81_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCI94567.1"
                     /translation="MLSIDIVGLTGACSYALDCIEAELVNIKNKHGKRVAYISIRMAE
                     YWKIQGDELQDLAMCALLHDNALTQYISEELKKDSVIDLKKDLSEEKTNLHCIYGEKN
                     ITKLPFKTDVSNVILYHHEHADGTGPFQKKWNEIPLFARIIHLADIIDIIRNSIDSDD
                     NSWDFMCQYLSQNKDSLFDSECVNAFLHVFTKESFMCLSDDSFETKLWKAIPREKLVF
                     DWEMCKDVADFFAKIVDYKSSFTSRHSIGVAEKASMLAQYMGYDSITVQKMYLAGALH
                     DIGKMAVGNEILEKPDKLTDDEFSKMKNHAGYTYLILSEVNDFEEIRDWAAFHHEKLN
                     GKGYPFGKTAAELNEPERMMACVDIYQALTEDRPYKKGLSHEKTCDILDDMAQKDFID
                     SDISKIIRECFGRNR"
     gene            1945..2380
                     /locus_tag="DHV81_09005"
                     /pseudo
     CDS             1945..2380
                     /locus_tag="DHV81_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742089.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
     assembly_gap    2271..2311
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2493..2652
                     /locus_tag="DHV81_09010"
                     /pseudo
     CDS             2493..2652
                     /locus_tag="DHV81_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514811.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing start; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2906..3244
                     /locus_tag="DHV81_09015"
     CDS             2906..3244
                     /locus_tag="DHV81_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94568.1"
                     /translation="MSFNWKASGLFAAGVAFGTAGIKILSSKDAKRAYTQCTAAALRA
                     KDCVMKTVNTVQENAEDILAEAKSINEEREAKAEAAKVSDASEDVCECAKADEQEAEQ
                     AEVTAETAEA"
     gene            3263..5344
                     /locus_tag="DHV81_09020"
     CDS             3263..5344
                     /locus_tag="DHV81_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCI94569.1"
                     /translation="MKFTIKHESRGRMRVHMEQYRMTYEQADTLLYVIHNHRNVTFVK
                     VYDRTADAVIEYVGDREQIIELLRHFHYESANVPQTVIKTSGRELNNSYQEKLIGSVV
                     WHYSKKLLLPWPIRTALTVGRSIKYIGIGLKCLLQRKIEVPVLDATAITVSLVTKDFS
                     TASSIMFLLGIGELLEEWTHKKSVDDLARSMSLNVSKVWLRTPENQEILVESSKIEKG
                     DKVVVHMGNVIPFDGEVLDGDAMVNQASLTGESVPVQRTVGNTVFAGTVVEEGEITIR
                     VKEVEGNNRFDQIVTMIEESEKLKSELEGKAEHYADKLVPWTLGATGLTYLLTRNVTK
                     AMSILMVDFCCALKLAMPISVLSAIREASLYNVTVKGGKFLEAVAEADTIVFDKTGTL
                     TKAHPTVVDVVNFNDEYSSDDMLRVAACLEEHFPHSMAKAVVDAASKKGLSHEEMHTK
                     VEYIVAHGIATSINGKRTVIGSYHFVFEDEKCVVPAGKEPLFESLPLYYSHLYLAIEG
                     KLSAVICIEDPLRDEAAAVVTSLKKAGISKVVMMTGDSERTASVIAKKVGVDEYYAEV
                     LPEDKAAFVEKEKAKGRKVIMIGDGINDSPALSAANVGIAISDGAEIAREIADITVGS
                     DDLYQIVTLKYISNALMKRIKSNYRKIVGFNSGLIALGVAGVLPPTTTALLHNGSTIL
                     ISVNSMKNLLE"
     gene            5729..5998
                     /locus_tag="DHV81_09025"
     CDS             5729..5998
                     /locus_tag="DHV81_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCI94570.1"
                     /translation="MKVEEVISRVAELCRQHDAQKVILYGSRAKGTALERSDIDIAVS
                     GVKDFDTLSDEVEDLPTLYSVDLLNLDTCRNDLLLEDIRQYGRKI"
     gene            5982..6389
                     /locus_tag="DHV81_09030"
     CDS             5982..6389
                     /locus_tag="DHV81_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCI94571.1"
                     /translation="MDAKFERRFKSFCNSLDALAEARQRDLSDSFVLSGTSAKFSITF
                     DLSWKVMKDILVQYYSITGFVTGSPREVLRESFKAKLISDDAWMDMLKVRNELAHDYD
                     CEVVRTHCNTIVEKYIDLFYDFKNTIDELLKVG"
     gene            6463..7470
                     /locus_tag="DHV81_09035"
     CDS             6463..7470
                     /locus_tag="DHV81_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94572.1"
                     /translation="MTNIEVEINDNYICVYERLNDDCIRLLHMYGKNPVCVVPDLIAG
                     MRVTELAEYCFSFKSMPEKLKTELGIDDILRPDMTELCDDYIERVILPDGMQKIGRLC
                     FYNCSRLSVLELPSDICDVDGDAFMNCTKLYMLVMRGSPKDKSCLKQILSQISTLVRV
                     RWADSDGNAIAQACFFEYDQTYDEIGPAHIFKLNMNGEGFRARQAFMDRVFVWKQYDE
                     IFSEAIAQESEDDLLDMAFYRLIYAYELSKEARQQFLENIVNHKKRLSELIIRKRDSG
                     LLQSFLELKDGEENFIADVLVVTDMLALAAQDEWSEGSAILHRFKKENLSVSRKRRFE
                     F"
BASE COUNT         2368 a         1170 c         1834 g         2326 t
ORIGIN      
        1 cgctccaaaa atacatcgtt cctaaaattt cctttttcgc cgggaataat cttgtatgga
       61 agcgcttcca tacgatcttt tacaggccat tcggtatgat atgccatacg cgcaacctct
      121 gtaaagattt tatgtctgat ttcctgaatt cgggtttcga cacctctcat tattgattcc
      181 tccttataaa ataaaatctc aattttcctt attgtatcag ataaaataag cattctcaat
      241 aaataggctg tactttttat gatacataaa tactagataa ttaatgaatt ttaaacctga
      301 ttttttgcgt ttctttgaaa ataaaggata atattaaatt tttaaaatag gaatgattac
      361 tgttttgtat tgactaaagt actataatac tatataataa ttatggacaa ttaatcctga
      421 ttaataagaa taaaaagtta tagaagcaat ttagatttta ttttttacac atggaaggag
      481 aaaaaagatg gaagtaaaat attatgtatg caatcactgt ggaaacatta ttgaaatggt
      541 aaaggataag ggcgtgccgg tagcagattt ttgtctttgc gatggtgagc aggtagaaga
      601 agtatatgca tattgcaatc tgcatggatt atggaaatgc taagtataga tattgttgga
      661 cttacaggag cctgttccta tgctttggac tgcatagaag cagagttggt aaatatcaaa
      721 aacaaacatg gaaaaagagt ggcttatata agtatacgca tggctgaata ttggaaaatt
      781 caaggtgatg aattacaaga tttagccatg tgtgctttac tgcatgataa tgctttgaca
      841 caatatattt ctgaggagct taaaaaggat tctgttattg acctaaaaaa agatttatct
      901 gaggaaaaaa cgaatctcca ttgtatatat ggtgaaaaga atattactaa acttccattt
      961 aaaacagatg tttcaaatgt gattttgtat catcatgaac atgccgatgg aactggccct
     1021 tttcaaaaaa agtggaatga aattccattg ttcgcaagga ttattcatct tgcagatatc
     1081 attgatatta taagaaatag tattgattct gatgataata gctgggattt tatgtgtcaa
     1141 tatctttccc aaaataagga tagtctattt gattcggaat gtgttaatgc ttttcttcat
     1201 gtattcacaa aagaatcgtt tatgtgttta agtgatgatt cttttgaaac taagctatgg
     1261 aaagctatcc caagagaaaa gctggttttt gattgggaaa tgtgtaaaga tgttgcagat
     1321 ttctttgcaa agattgtaga ttataaatct tcttttacaa gcagacattc tataggagta
     1381 gctgaaaaag catccatgct tgcccaatat atgggatatg attcaatcac tgtacagaaa
     1441 atgtatttag ctggtgctct gcatgatata ggaaaaatgg cggtaggcaa tgaaattctg
     1501 gaaaaaccgg ataagcttac ggatgatgaa ttttctaaaa tgaaaaatca tgctggttat
     1561 acatacctaa tattatcaga ggttaatgat tttgaagaaa tacgagactg ggcagctttc
     1621 catcatgaaa aattaaatgg aaaggggtat ccttttggaa aaactgctgc tgaattaaat
     1681 gaaccggaac gtatgatggc atgtgttgat atttatcagg ctcttacgga agatagacct
     1741 tataaaaaag gattatctca tgaaaaaaca tgtgatatcc ttgatgacat ggcacagaaa
     1801 gattttattg attcggatat ttcaaagata attagagaat gttttggaag aaaccgatga
     1861 tatataagat aatagtgttt tccattgtat acttgtatta cgtggatttc aaaagaagaa
     1921 taagaatgaa agtaggatta atttatgtat gaaatgaaac cagaatatta tatcggcata
     1981 gatatgatag acgaagagca taagcagttg tttaaatatg cagatgatgc atatgaactg
     2041 cttcatgacg agaatacacc ggataaatat gatagaattg acatgatact tgaagacctt
     2101 cgtgattata cagcgaaaca ttttaatgat gaagagcagt atatggagtc tattaattat
     2161 aaaaaattat ttactcaaaa aattcagcat caggaattta tacacaaact tgatgaattt
     2221 attgaacatc acaatgatga aataaaagat caggatgaac agattatggg nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacagagtgg ttagttaatc acatcctata
     2341 tgttgatggt caaattgcag aatttgtatc aaaaatgtaa tcttttgaat tgctaataaa
     2401 tattatattt gatggtcata atatctcttc agttggagag aatacctttt gatcatcaaa
     2461 tccacctgtt tgtttgacgg agtaatggat atgaaaatca gggaaagggc tatgggaaag
     2521 cactaattga ttttcttgcc agtaaatatg cagatgaata ttccgaattt ctttacggac
     2581 aattatgatc acccaattta tgagtgtggt gtacagttaa tagatatggt atatttgcaa
     2641 agatgtttat aacttccttc ttgaaaatat acatagtgcg ttttttcgag tccattcgat
     2701 gttgctatgc ttttgtgtat gtattaattg aagtgataaa ctttatcaat aagtccggga
     2761 ggacgcataa taagggcgtt tgagggcctg aattagcaag aactgtatga gattaggctt
     2821 gaataacagt atttccggga aatataatgt ttcttgtaag taaactttaa ccagaatttt
     2881 tgtaaatata caggaggaaa acattatgtc atttaattgg aaagcttcag gtttattcgc
     2941 agcaggtgtt gcttttggta cagcaggtat caagatttta tcaagcaagg atgctaagcg
     3001 tgcttataca cagtgtacag cagcagcgct tcgtgctaag gattgtgtta tgaagactgt
     3061 taatactgtt caggagaatg cagaggatat tcttgctgag gctaagagca tcaacgagga
     3121 gcgcgaggct aaggctgagg ctgctaaggt ttcggatgca tctgaggatg tatgcgagtg
     3181 tgctaaggct gatgagcagg aagcagagca ggctgaggtt acagcagaga ctgctgaggc
     3241 atagaagata agaggcggtc atatgaaatt tacgattaag catgagtcaa gaggccgtat
     3301 gcgtgttcat atggagcagt accgcatgac atatgagcag gcggacactc tgctttatgt
     3361 gattcacaat cacaggaatg ttacctttgt aaaggtttat gacaggacgg ctgatgctgt
     3421 gattgagtat gtaggagaca gagagcagat tatagagctg cttcgccact ttcattatga
     3481 gagtgccaac gtgccacaga ctgttatcaa gacatcagga agagagctta ataattcata
     3541 tcaggaaaaa cttatcggaa gtgttgtctg gcattacagt aaaaagctcc tgctgccttg
     3601 gccaatcagg acagcgctta ctgttggacg ttctataaag tatattggaa ttgggcttaa
     3661 gtgtctgttg cagagaaaaa tagaggtgcc ggtgcttgat gctactgcca tcacggtttc
     3721 tcttgtcaca aaggattttt ctaccgcaag ctctattatg tttctgcttg gaataggtga
     3781 gcttttggag gaatggacac acaagaagtc tgttgatgac ctggccagaa gcatgtcgct
     3841 taatgtgagc aaggtatggc ttaggacacc tgagaatcag gagatactcg ttgagtcctc
     3901 aaagattgaa aagggcgaca aggttgttgt ccacatggga aatgtcatac cgttcgatgg
     3961 agaggtctta gatggcgatg cgatggtcaa tcaggcttcc ctgacaggtg agtcggttcc
     4021 ggttcaaaga actgtcggca atactgtgtt tgcaggaact gttgtcgagg agggcgagat
     4081 tactatcagg gtcaaggagg ttgaaggaaa caaccgtttt gaccagattg ttactatgat
     4141 agaggagtca gaaaagctta aatctgagct ggagggaaag gctgagcact atgctgacaa
     4201 gcttgtgccg tggacgcttg gagctacagg acttacatat cttctgacaa gaaatgtgac
     4261 aaaggctatg tcaattctca tggtggattt ttgctgtgca ctcaagcttg ctatgccaat
     4321 ctcagtgctt tctgctatca gagaggcaag cctttataat gtaacagtaa agggaggaaa
     4381 gttccttgag gctgttgcgg aggctgatac catcgtcttt gacaagacag gtacactcac
     4441 aaaggcacat ccgactgtgg ttgatgtggt gaacttcaat gatgagtatt catctgatga
     4501 catgcttcgt gtagcagcct gtctggagga gcatttccct cattccatgg caaaggcagt
     4561 ggtagacgct gcttctaaga aggggctttc ccacgaggaa atgcatacaa aggttgagta
     4621 tattgtggct catggaattg caacctccat caatggcaag cgtactgtta tcggaagcta
     4681 tcatttcgtc tttgaggacg aaaaatgtgt tgttcctgca ggaaaggagc cactgtttga
     4741 gtcgcttccg ctgtattatt ctcacctgta tctggctatt gagggtaagc tttcggcagt
     4801 tatctgtatt gaggatcctc tgcgtgatga ggcagcggcg gttgttactt cattaaagaa
     4861 ggccggtata tcaaaggttg tgatgatgac aggcgacagt gagcgtacag cttctgtcat
     4921 tgcgaaaaaa gtaggtgtag atgaatatta cgccgaggtt ttacctgagg acaaggctgc
     4981 ctttgtggag aaagaaaagg ctaagggcag gaaggtcatt atgattggtg acggcataaa
     5041 tgattcaccg gctctttcag cggcaaatgt cggtattgca atcagtgacg gtgctgaaat
     5101 tgcacgcgag attgctgata ttacggtggg ctctgacgat ctctaccaga ttgtaactct
     5161 taaatatata agtaatgctc ttatgaaacg tataaagagc aactatcgaa agattgttgg
     5221 ttttaactca gggctcattg ctcttggtgt ggcaggtgtg cttccgccga ctacgacagc
     5281 acttttgcac aatggatcga caattcttat cagtgtgaac agtatgaaga atctgcttga
     5341 gtagattttg gatgtcaatg tttattgatg tagtacgggg cgctgccgcg ggtaccgtgg
     5401 tggcacacgt actatcccgc gtgctacgct tcgtgggtaa gatactatac gaaattatgc
     5461 ttgaagggtt tctatgcttg acgaaaccgt cgaaacgcgc cgtccatggc gcggttctcc
     5521 tcgcccctgc gtccttgcag gggcgtttct gcgcttcatg gcataatttc tacagtatct
     5581 aactcactgc gctattcgca cacgtgatag tacgtgtgcc accgcggtgc ccgcggcagc
     5641 gccccttacg ttgtggtgga aataatgtag gaattgtgat aaactaactc atatgtagtg
     5701 cagattattt ttatatgagg ttagattagt gaaggttgaa gaggttataa gcagggttgc
     5761 agagctttgc agacagcatg atgcccaaaa ggtaatttta tatggttcaa gagctaaagg
     5821 tactgcattg gaacgtagtg atattgatat agcggtatcg ggagtgaagg attttgatac
     5881 gctttctgat gaagtggagg acttacctac cttgtacagt gttgatttac tgaatttgga
     5941 tacatgcagg aatgatttat tattggagga tatcagacag tatggacgca aaatttgaga
     6001 gacgttttaa atctttttgt aattcactag atgcgttagc tgaggcaagg cagagagacc
     6061 tttccgattc gtttgttttg agtgggacaa gtgctaaatt tagcattact tttgatttat
     6121 cgtggaaggt aatgaaagat attctggtac aatattattc gataaccggg tttgttacag
     6181 gttcaccgcg agaggtgtta cgtgaatcct tcaaggcaaa gctgatttct gatgatgcgt
     6241 ggatggatat gcttaaggtc agaaatgaac ttgcacatga ctatgattgt gaggttgtaa
     6301 ggacacattg taatacaatt gttgaaaaat atattgattt gttttatgat tttaagaata
     6361 cgatagatga attattgaag gttggataaa tttttacgta tatatatcta atgcacaaaa
     6421 ttaacacaat atagtaataa aaacatagaa ttaaagatag cgatgacaaa tatagaagtt
     6481 gaaataaacg ataattatat atgtgtatat gagaggctta atgatgattg cattaggctt
     6541 cttcatatgt atgggaaaaa tccggtgtgt gtggtgccgg atttgattgc cggcatgaga
     6601 gtgacagagc ttgcggagta ttgtttttcc ttcaagagca tgcctgaaaa attgaaaaca
     6661 gagcttggga ttgatgatat tctgcgtcct gatatgacgg agctatgtga tgattatatc
     6721 gagagggtca tattgccgga cggaatgcaa aagattggca ggctatgctt ttacaattgt
     6781 tcaaggctgt ctgtgcttga gctgccatcc gacatatgtg atgtggatgg tgacgctttc
     6841 atgaactgca caaaacttta tatgcttgtg atgagaggct caccgaagga taagtcgtgt
     6901 cttaagcaga ttctttcaca gataagcact cttgtgaggg ttagatgggc tgacagcgat
     6961 ggaaatgcga ttgcgcaggc atgttttttc gagtacgacc agacctatga tgagattggt
     7021 cctgctcata ttttcaagct gaacatgaac ggagaaggct ttcgtgcgag acaggctttt
     7081 atggaccgcg tgtttgtgtg gaagcagtat gatgaaatat tttcagaggc gattgcgcag
     7141 gagagtgagg atgacctctt agatatggct ttttacaggc ttatttatgc gtatgagctt
     7201 tcaaaagaag cgagacagca gtttttggag aatattgtca atcataaaaa aaggctgtct
     7261 gagcttatca taagaaaaag ggattcgggt ttgctgcaga gttttctgga gcttaaagat
     7321 ggtgaggaga attttatcgc agatgtcctg gttgttactg atatgcttgc acttgcagca
     7381 caggatgaat ggagtgaggg cagtgcgatt ttgcatagat ttaagaagga aaatctgtcg
     7441 gtatccagga agaggcggtt tgaattttaa atttaatcat aaatgagggg caaaatacct
     7501 tttgaccctc atatcatgtg tatagtgtaa taaattttaa aaatattgca gtagaataat
     7561 actcgcacca ttggtgcaag aaatggagaa gtatatggga aaggaggcag cttcatgaaa
     7621 aaacatatcc agacagattc agagtggcag gaggaaatgt cacagaaaat aatagatgaa
     7681 atacagtgcg agttgtatct cgacatgcct tttatgaagc tggcgctctc ggctctttc
//
LOCUS       DPUF01000260            5520 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_20278, whole
            genome shotgun sequence.
ACCESSION   DPUF01000260 DPUF01000000
VERSION     DPUF01000260.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_20278"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="DHV81_09040"
     CDS             <1..237
                     /locus_tag="DHV81_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94573.1"
                     /translation="EIFEHVSPDGIRLTGSNMLMPLKTAALIIKLDSQQKKNITELCN
                     TCASCKNLSCPSRKAHGPKLPHTYGAMQIFNIRH"
     gene            complement(227..>2599)
                     /locus_tag="DHV81_09045"
     CDS             complement(227..>2599)
                     /locus_tag="DHV81_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase P"
                     /protein_id="HCI94574.1"
                     /translation="LTDEEVRQRVEEGLTNRADISTDKTTKEIVISNVFTYFNLIFLV
                     ITILLIMVGSFRNLTFLPIIIGNTVIGIVQEIRAKKTLEKMSLLNALHADVIRNGSVK
                     QISTEELVKDDVILLTAGKQICADAVVISGNIQVNESLLTGEADEVEKTEGSTLMSGS
                     FVVSGECYARLEKVGNESYISKLSLEAKSMGGKEQSEMIRSINLIVKWVGIVIIPIGL
                     ILFWQSHFVNGESITKSVTSTVAAIIGMIPEGLYLLTTVALALSTMKLARKKVLLHDM
                     KSIETLARVDVLCVDKTGTITEPDMKLKDIFLCKNSGADGTQTALTIDELKSLISDYA
                     NASVDNNATMLALKAYAADALTNNTSALHRTALSQQAFSSSLKYGSVTFSDGTYLLGA
                     PEFIMHDDFSRIEEEIIPYADKGDRVLLFARYDGENVENGINGSVTPLGFVALANPIR
                     ENAVKTFEYFKSQGVAIKVISGDNPRTVSQIAIQAGIENAESFVDAATLDTEDKIADA
                     VNKYTVFGRVTPKQKKQLVKALQAKGHTVAMTGDGVNDILAMKDADCSVAMASGSEAA
                     AQAAQVVLLDSDFAHMPDVVYEGRRVVNNIQRSASLFLVKNIFSLLLSLFSVILMVTY
                     PLEPAQVSLISMFTIGVPGFLLALEPNKDRIKGHFITNVMLKALPGGLTDVIAVGALV
                     VCGEVFCISDASIGTIATLVLSVVGFMILFKISEPLNGMKYAVIIGNIAGLVFSGFFL
                     KKLFALTDLSNICILLMIVFGFAAESLFRNLTLLVEKLRGSYEKKKGFNV"
     assembly_gap    2600..2763
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2884..>3201)
                     /locus_tag="DHV81_09050"
     CDS             complement(2884..>3201)
                     /locus_tag="DHV81_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper ABC transporter permease"
                     /protein_id="HCI94575.1"
                     /translation="FTTGLAVNDSSTGASIVVFGTPYVVDDSYDNAVSGNNADMFKDV
                     ITSMTGNVELASSVIPVKDYTLSNITINTLQAVITGLIIMVAVPILLIIIGIVVWAMR
                     RKK"
     assembly_gap    3202..3320
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3321..5014)
                     /locus_tag="DHV81_09055"
     CDS             complement(<3321..5014)
                     /locus_tag="DHV81_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper ABC transporter permease"
                     /protein_id="HCI94576.1"
                     /translation="MLAIFKREFKSYFQNVIGWLFVAALLAVYGLYFYVYNLKNGYPY
                     ISYDLKGIGFIMMIAVPILTMRSLSDEKKTKTDQLMLTSPVSVGRIVAGKYFAMAAVY
                     TIDIALFALSPLVLSIYGKVALSEAYVALFGYWLYGLSCIAVGIFISSISESVIISAI
                     LTFAALFLSYMMQSITGLISSSGNLLTKVLNCFDLYTPFENFVSGCFSVTSAAYYVTV
                     ILLLCFLTTQSIQKRRWAFSKKMIGTGAFSAGMIVVMCAICVVVNLVLTALPAKYTSI
                     DCSATKLYSLTGDTKDRVSKLDEDITIYVLNSKKSKDAKIDETINRYKDLSSHIKVKY
                     VDPATSPKFYQDYTDTTPTTNSLIIESKNRSKVIDYNDIYEYDSLSYYYGYQSQSSIT
                     GYDAEGQITSAIEYVTMDADELPVIYQITGHNETEIGSNFQSVVSKANANLKSLELFN
                     EEKVPEDATAIIINSPTVDFNEEDAQKVIDYLNGGGKAMIIGCYAYNDELTNFNKILA
                     AYNVSFKTGVVAENDSSKYYQNPLYLLPTVETTDYTSDATDGYVFMAGSCAISYPE"
     gene            complement(5014..>5520)
                     /locus_tag="DHV81_09060"
     CDS             complement(5014..>5520)
                     /locus_tag="DHV81_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI94577.1"
                     /translation="LLGYPEIIILDEPTVGLDPKQIIEIRELIKKLGENHTVILSSHI
                     LSEISAVCDHVFIISKGKLVASDATENLINLMSKNQEINLVLKSDEIGARGMLEKIVN
                     VDKVTFEKSEEEKTVKAKVIAKANCDIREEIFELASAMHMPILEMHTVVKSLEDVFLE
                     LTGEGDEK"
BASE COUNT         1459 a         1275 c          931 g         1572 t
ORIGIN      
        1 gagatttttg agcacgtaag ccccgatggt atacgtctca ccggttcaaa tatgctcatg
       61 ccacttaaga cagctgctct cataataaag ctcgactcac aacagaaaaa aaacatcaca
      121 gagctctgca atacctgcgc aagctgtaag aatctgtcgt gcccatcaag aaaggcccat
      181 ggtcctaagc tgccacacac ctatggtgcc atgcagattt ttaatatcag acattgaagc
      241 ccttcttttt ctcgtaggag cctctcagct tttcaaccag aagtgtcaga ttcctaaaca
      301 gtgactcagc cgcaaaacca aacactatca tgagaagaat acatatattt gacaaatctg
      361 tcagtgcaaa cagtttcttt aagaagaaac ccgaaaatac cagacctgct atatttccga
      421 taataacagc atatttcatt ccatttagag gctcactgat tttaaagaga atcataaagc
      481 ccactaccga aagtacaagt gtcgctatag ttccgatgct tgcatccgat atacaaaata
      541 cctctccgca tacaaccagc gctccgacag ctattacatc cgtaaggccg ccgggcagtg
      601 cctttagcat aacattggtg ataaagtgtc cctttatcct gtccttattc ggctccaatg
      661 ccagcaagaa tcccggtact cctattgtaa acatactaat gagcgatacc tgtgcaggct
      721 caagaggata cgtaaccatc aggataaccg aaaacagtga tagtaacagt gagaatatat
      781 ttttcaccag aaacaggctg gctgagcgct ggatattatt taccaccctg cgtccctcat
      841 ataccacatc cggcatgtgt gcaaagtcag agtccaaaag gacaacctgt gctgcctggg
      901 ctgccgcctc acttcccgat gccatagcca cactgcagtc tgcatccttc atggcaagta
      961 tatcattgac tccgtctccg gtcatcgcaa ctgtatgccc tttagcctgc aatgccttta
     1021 caagctgttt cttctgcttt ggtgtcactc gcccgaatac agtatattta ttcactgcat
     1081 cggctatttt atcctcagta tcaagtgtgg ccgcatcgac aaagctttct gcattttcta
     1141 ttcctgcctg tatggcaatc tgtgaaaccg tacgcggatt atcacctgaa ataaccttta
     1201 tcgcaacacc ctgactcttg aaatactcaa aggtctttac cgcattctct ctgataggat
     1261 tggcaagtgc cacaaatcca agcggagtaa cgctgccatt tatgccattc tccacgtttt
     1321 ccccatcata ccttgcaaac aaaagtactc tgtcgccttt gtcagcgtat ggtatgattt
     1381 cttcctcaat gcgagaaaag tcatcgtgca tgataaattc cggtgctccg agcagatatg
     1441 ttccgtcaga aaaagtaaca ctgccatact taaggctaga cgaaaacgcc tgctgtgaaa
     1501 gtgctgtacg atgcagtgca gatgtattat ttgtcaaagc atccgctgca tatgccttta
     1561 gtgcgagcat agtcgcattg ttgtcaaccg aggcattcgc ataatccgaa atcaggctct
     1621 taagctcatc tattgtcaaa gctgtctgtg tgccatctgc tccactgttt ttacagagga
     1681 aaatatcctt aagcttcata tcaggctcag taattgtgcc cgttttatcc acacaaagca
     1741 catccacacg cgcaagcgtc tcaatacttt tcatatcatg aagcaaaacc ttttttcttg
     1801 caagcttcat agtgcttaag gcaagcgcca ctgtggtcag cagatataag ccttccggta
     1861 tcattcctat aatcgctgca actgtcgatg taacgctctt tgtgatagac tcgccattca
     1921 caaaatgact ctgccagaat aatataagcc caatcggaat aataactatg ccaacccatt
     1981 tgacgatcaa attgatggag cgtatcatct ctgactgctc cttgccgccc attgattttg
     2041 cctcaaggct cagctttgag atatatgatt cattaccgac cttttcaagc cgtgcatagc
     2101 actctcccga tacaacaaag cttcccgaca tgagagtgct gccctctgtc ttttctacct
     2161 catcggcttc acctgtgagc agtgattcat ttacctgtat atttccggat atcacaaccg
     2221 catccgcaca aatctgcttt cctgcggtaa gtaaaatcac atcgtccttt acaagttcct
     2281 ccgtactaat ctgctttacg ctgccgtttc ggatgacatc cgcatgcaaa gcattcagta
     2341 aactcatttt ttcaagagtc ttttttgcgc ggatttcctg aacgattccg attaccgtat
     2401 ttccaataat tataggcaga aatgtcagat ttctgaaaga acctaccatt atgagaagta
     2461 tcgttatgac aagaaatata aggttaaaat atgtgaaaac gtttgaaatc acaatttcct
     2521 ttgtcgtctt gtctgtagat atatcagccc tgttggtaag tccttcctcc actctctgcc
     2581 ttacctcttc atcggtaagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnntatttca ttaaaaaaag atgaactatt tattaatccg ttgctactca ctttcagtct
     2821 cagtactctc actgtcttct gtttctacca tatcatcatt tcttatgcgc ttttacctgt
     2881 aatctacttc tttctcctca ttgcccatac aacgattcct atgataataa gcagtattgg
     2941 tacagctacc attatgataa gtcccgtaat aaccgcctgc agcgtattta tagtgatatt
     3001 gcttaaggtg taatccttca ccggtatcac acttgatgca agctcaacat ttccggtcat
     3061 tgatgtgata acatccttga acatgtctgc attgttgccg gaaacggcat tgtcatatga
     3121 atcatcaacc acataaggcg taccaaacac aacaatcgat gcgccggtgc ttgaatcatt
     3181 tacagcaaga cctgtagtaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn tcctcaggat aactgattgc acagcttcct gccatgaata
     3361 catatccgtc tgttgcatct gaggtgtaat ctgtcgtctc aaccgttgga agcaggtaga
     3421 gaggattctg ataatatttt gagctgtcat tttcagctac cacaccggtc ttgaagctga
     3481 cattgtaagc cgcaagtatc ttgttaaagt ttgtaagctc gtcattatat gcgtagcagc
     3541 cgataatcat tgcctttcca ccgccattta aataatcaat caccttctgt gcatcttcct
     3601 cgttgaaatc aacagtcgga gaattaataa taatggctgt tgcatcctcc ggcacctttt
     3661 cctcgttaaa gagctctaag ctcttaagat ttgcatttgc cttgcttaca acactctgga
     3721 aattgcttcc tatttccgtt tcattgtgtc ctgttatctg ataaattacc gggagctcat
     3781 ctgcatccat tgtgacatac tctatagcac ttgtaatctg tccctcggca tcatagcctg
     3841 tgatgctaga ctgcgactgg tatccgtaat aatatgataa gctgtcatat tcatatatat
     3901 cattgtaatc aatcaccttt gaacggtttt tgctctcgat gataaggctg tttgtggtag
     3961 gtgtcgtgtc tgtgtaatcc tgataaaact tcgggcttgt cgccgggtct acatatttta
     4021 cctttatatg actcgaaaga tccttgtatc tgttgattgt ctcatctatc tttgcatcct
     4081 ttgatttctt ggaattcaaa acatagattg tgatatcctc atcgagctta cttaccctgt
     4141 cctttgtatc gcctgtgagc gagtacagct ttgtagccga acagtcaatt gatgtgtact
     4201 tagcaggaag cgctgtcagt acaagattta caaccacaca gattgcacac attacgacaa
     4261 tcattcctgc tgaaaacgcc cctgtgccta tcatcttctt tgaaaatgcc catcttctct
     4321 tctggataga ctgcgtcgta aggaagcata aaagcagaat aaccgtcaca taataagctg
     4381 ccgaggtgac actaaagcag ccactcacaa aattctcaaa tggtgtgtac aaatcaaagc
     4441 aattaagcac ctttgtgagc agattcccac ttgacgagat gagccctgtg atggactgca
     4501 tcatgtatga aaggaataat gccgcaaatg taagtattgc agatattatc acactctcag
     4561 atatggacga tataaaaatt cccacggcga tgcatgataa accgtaaagc cagtagccaa
     4621 acaaagccac atacgcttca cttaatgcca ccttaccgta aattgacagc acgagcggtg
     4681 aaagtgcaaa aagtgctatg tctatggtgt atactgctgc catcgcaaaa tattttcctg
     4741 cgacaatcct tccgacagat acaggcgatg tcagcattag ctgatctgtc tttgtctttt
     4801 tctcatcaga aagcgatctc atcgtgagaa ttggcactgc aatcatcata ataaaaccga
     4861 ttccctttaa atcatatgag atatacggat aaccgttttt cagattgtat acataaaaat
     4921 atagaccata cactgcaaga agtgctgcaa caaacagcca tcctataacg ttttggaaat
     4981 atgatttgaa ttctctttta aatattgcaa gcattatttc tcatctcctt cccctgtaag
     5041 ctcaaggaat acgtcctcga gtgacttcac aacagtatgc atctcaagga ttggcatatg
     5101 cattgcactt gcaagctcaa atatttcctc acgtatatcg cagtttgctt ttgctataac
     5161 cttagccttc actgttttct cttcctcaga cttttcaaat gtgaccttat caacatttac
     5221 tatcttctca agcattcctc ttgcaccaat ctcatctgat ttgaggacaa ggttaatttc
     5281 ctggttcttt gacataagat taatcaggtt ctctgtggca tcacttgcta caagcttacc
     5341 ctttgatatg ataaacacat gatcacacac cgcacttatc tcacttaata tatgcgagct
     5401 aagtatcacc gtatgatttt ctccaagctt tttaataagc tcacgaatct ctattatctg
     5461 ctttggatca agtcccactg taggctcatc cagtatgatg atttccgggt agccaagaag
//
LOCUS       DPUF01000261            4354 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_20627, whole
            genome shotgun sequence.
ACCESSION   DPUF01000261 DPUF01000000
VERSION     DPUF01000261.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_20627"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(372..1763)
                     /locus_tag="DHV81_09065"
     CDS             complement(372..1763)
                     /locus_tag="DHV81_09065"
                     /inference="COORDINATES: protein motif:HMM:PF12773.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94578.1"
                     /translation="MLELSTKLIACKKYLNVLKERRITMYCNNCGQEVPNNSGFCPNC
                     GAKINPQNQTAQDAQSTEPRTIKLRCKNCNATMEVDASKQEAVCPYCGAKEKILDSDA
                     VAVEKIKSNTYKEMEYARMEKEEKQNAKNQKEQEKKAYSKSFLSKFTIVMTFICLICA
                     FTAFGSKHILAGIIALIQTVIFAVSWLMGKQIIGENKKAVYKALALLGCLLIIPFSLS
                     NQTHNSDRSDTAKNDFSKITWPTSELAKLIPQPSSNIGKINLDSSNYLSIDVGNITEA
                     DFKEYAQKCSDAGFNVAYKKNDTYYIASDTNGNRLNITYDKDEIMCIQLRAADTSDDS
                     ENSEADTAVANTGTAITTDNSGSDNPATTDSGDTQNSTTDSGSVTPELKEFLDSYESF
                     MDEYIAFMQKYNSSGDTLSMATDYAKMLAKYAEFSKKADAYNTDEMSAEDAAYYLDVM
                     NRVNKKLADAALN"
     gene            complement(1856..>2797)
                     /locus_tag="DHV81_09070"
     CDS             complement(1856..>2797)
                     /locus_tag="DHV81_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCI94579.1"
                     /translation="VFGTGNATQVNTITTAIDSALYNYDIISKESASTFNLIIGIVLA
                     ALIALILLGGIKRIGQVTEKLVPFMAVMYILLALGVVIVNFKAIPGVFGAIIEGAFNP
                     SAVTGGAVGSFFMSMKKGVSRGIFSNEAGLGTGSIAHACADTKKPVKQGFFGIFEVFV
                     DTIVICTLTALVILCSGVPVGYGEAAGAELTISGFTSTYGGWVSIFTAVAMCCFAFST
                     IIGWGLYGTRCIEFLFGTKANKPFMVVYSLVAIVGATMNLGLMWSIAETFNGLMVIPN
                     LIAVFLLSGVVVKLVKEYFAGEGAASTLKNSREEAKS"
     assembly_gap    2798..2908
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2909..3315)
                     /locus_tag="DHV81_09075"
     CDS             complement(<2909..3315)
                     /locus_tag="DHV81_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCI94580.1"
                     /translation="MLSVIEKVNDVVNNFIWGVPAMICIIGVGLLLSFRTGFIQIRKF
                     PYAMKVTLGRMLKKREASDGALTPFQAVCTALAATVGTGNVAGVAGAIAIGGPGAVFW
                     MWISALLGMCTKFSEVTLAVHFHERNANGDLVGG"
     gene            complement(3487..3693)
                     /locus_tag="DHV81_09080"
     CDS             complement(3487..3693)
                     /locus_tag="DHV81_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94581.1"
                     /translation="MKSTKDSLLEDIKAEFAEVNAMMEALEAKEPEDEASEAYDKWIE
                     ECEQLAIESESLMTLIDYKMTQGL"
     gene            complement(3786..4334)
                     /locus_tag="DHV81_09085"
     CDS             complement(3786..4334)
                     /locus_tag="DHV81_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCI94582.1"
                     /translation="MEYFDIVDEQGNPTGQTVERKQAHRNNILHRTAHVWIVRRRGNS
                     IQILLQKRCMEKDSFPGCYDISSAGHIPAGVDYIPSALRELKEELGLTIEPKQLIDCG
                     LHRHHADEVFHGERFLDNQISKVFLLWLDVDEADITVQKEEIDSVKWFDYAECKQDVI
                     NGTIPNCIDVAELEMLEPHFAE"
BASE COUNT         1083 a         1003 c          786 g         1371 t
ORIGIN      
        1 tgctgtggtg tcgtatttac agaaatataa tgctttgagt tgattgtagc acaggagaga
       61 cattttgtca ttaaatttct cagagtttct tagcatccct tagcatttct tatcatatgc
      121 taagatttca tattaatttt ttaaattcta caaaccggat ttgattaata accccaattg
      181 attattttct ttgtaagcat agcataatct ttatcccctt tctatttttt tagacacatt
      241 atatttatga aaaactacat attttataaa aatcgataca atttttttaa ctataaatga
      301 gtctgattcg atatcaaatc agactcattt ttatgattaa taacccctat atttcactta
      361 caatgaacac cttaatttaa tgcagcatct gccagcttct tattcacacg gttcattacg
      421 tcgagatagt aagctgcatc ttcagctgac atttcatcag tgttgtaggc atcagccttc
      481 tttgagaact cagcatactt agcaagcatc tttgcatagt cagttgccat actaagtgtg
      541 tcacctgagc tattgtattt ctgcatgaaa gcaatgtatt catccatgaa tgactcgtag
      601 ctgtctaaga actctttgag ctccggtgtt acagaaccag agtccgttgt agagttctga
      661 gtatcgccgg aatctgttgt ggcagggttg tcagaaccgg aattatcagt tgttatggct
      721 gtgccggtat tggctacagc agtgtcagcc tcgctatttt ctgaatcatc tgaagtgtca
      781 gctgccctaa gctgtataca cattatttcg tctttatcat atgttatatt cagacggttt
      841 ccattggtat ctgaagcaat gtaataggta tcattctttt tataggccac attaaatccg
      901 gcatcactgc atttttgcgc atattccttg aaatcagctt ctgttatatt tcctacatct
      961 attgaaaggt agttagagct atcaagattg atttttccta tatttgatga tggctgtgga
     1021 atcagcttgg caagctctga ggtcggccat gtaattttgg aaaaatcgtt tttcgccgtg
     1081 tcagatctat cagaattatg agtctgatta ctcagcgaaa acggaataat gagcaaacat
     1141 ccaagtaacg caagtgcctt gtagacagcc tttttatttt ctccgataat ctgcttgccc
     1201 atgagccatg ataccgcaaa tatcacagtc tggataagtg cgataattcc tgccaggatg
     1261 tgctttgagc caaaagcggt gaaagcgcaa atcagacaga taaacgtcat aacgatagtg
     1321 aatttactta gaaagctctt gctataagct ttcttttcct gctctttctg atttttagcg
     1381 ttctgctttt cctccttctc catgcgcgca tattccattt ccttgtaggt gttgctcttg
     1441 attttctcaa cggcaaccgc atcgctgtcg agtatctttt ccttagctcc acagtatggg
     1501 catacagcct cctgctttga ggcatccacc tccatagttg cattgcagtt tttgcatcta
     1561 agcttgatgg tgcgtggctc tgtgctctgt gcgtcttgag ctgtctgatt ctgtggattt
     1621 atttttgctc cacagttcgg acaaaatcct gagttgttag ggacttcctg tccgcaattg
     1681 ttacagtaca tagtgattct cctctctttt agtacattta aatacttttt acatgctatt
     1741 aatttagtac ttaattcaag catagaactg cactttgagt gcagtcaaag tattttttat
     1801 gagtatgctt cactatagaa aatgtttttt tcgcgaaaaa aagtccttct cttatttagc
     1861 tttttgcctc ttcccggcta ttcttcaagg tagatgcagc tccctctcct gcaaaatatt
     1921 ccttcaccag ctttacaact acacccgaaa gcaaaaatac cgcgataagg tttggtatga
     1981 ccataaggcc attgaatgtc tccgctatgc tccacatcag tccgaggttc attgtcgcac
     2041 cgactatcgc aacaagcgaa tatactacca tgaacggctt atttgcctta gttccgaata
     2101 agaactctat gcatctggta ccgtaaagac cccagcctat gatggttgaa aatgcaaaac
     2161 agcacatggc aaccgctgta aagattgata cccagccgcc gtaggtggat gtaaatccgc
     2221 tgatggtaag ctctgcgcct gctgcttctc catagcccac aggaactccg ctgcaaagta
     2281 tgacaagcgc tgtgagtgta catatcacga tagtgtccac aaacacctca aatatgccga
     2341 agaagccctg ctttactggc tttttggtgt ctgcacaggc atgtgcgata gagcctgttc
     2401 caagacctgc ttcgtttgaa aaaattcctc tggaaactcc ctttttcatg ctcataaaaa
     2461 agcttcctac tgctccgcct gtcactgctg acggattgaa cgcgccctct attatggctc
     2521 cgaatacgcc cggtatagct ttaaagttga cgattactac tccaagtgcc agcagaatat
     2581 acatcactgc cataaatggc acaagctttt cagtaacctg gcctatacgc ttgattcctc
     2641 caagcagaat aagcgcaatg agagcggcaa gcactattcc tattatgaga ttgaacgttg
     2701 atgccgactc ttttgaaatt atatcgtaat tatataaagc tgaatcaatc gctgtggtta
     2761 tggtgtttac ctgtgtagcg tttcctgtac cgaacacnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gcctaccagg tcgccgtttg catttctctc
     2941 gtgaaaatgt acagcaagtg tgacctccga gaacttggtg cacataccaa gaagtgctga
     3001 aatccacatc cagaacaccg ctcccggtcc gcctatcgcg atggctcccg caactcctgc
     3061 cacatttcct gtaccgaccg tcgccgcaag tgccgtacac actgcctgaa acggtgttag
     3121 cgctccgtca gatgcctcac gttttttgag catcctgcca agcgtaacct tcatcgcata
     3181 tggaaacttt ctaatctgaa taaatcccgt tctgaagctc aaaagcagcc cgacacctat
     3241 gatacatatc atcgcaggca cgccccagat aaaattgttg accacgtcat tgactttttc
     3301 tattactgat aacatctttt tctcctactg aatttagtgt aacgtataaa gtataacacg
     3361 gtttgtacaa ttttttatat atttaatttc atttttttga agtttttctt tataaaaaac
     3421 acccggatat agcaatttat ctatatccgg atgtttattt ttgtataata tatacaggat
     3481 atccctctac agtccctgtg tcatcttgta atctataagt gtcatgagcg attctgactc
     3541 gatggcgagc tgctcacact cctcaatcca cttatcgtag gcttcactcg cctcatcctc
     3601 cggctccttt gcttcgagcg cctccatcat ggcattgacc tcggcaaatt ctgccttgat
     3661 atcctctaag agagagtctt ttgttgattt catagtatcc tcccaatttg atttaacgta
     3721 aaatatattt ttatattctc atgatagctc gaatagagcc atcgccacac gatttcacaa
     3781 aaatttcatt ctgcaaagtg tggctcaagc atctcaagct ccgccacatc tatgcagttt
     3841 ggaatcgtgc cgtttattac gtcctgctta cactctgcat agtcaaacca ctttacagag
     3901 tcgatttcct ccttctgcac tgtaatatcc gcttcgtcca catcgagcca cagaagaaac
     3961 accttgctta tctggttgtc caggaatctt tctccgtgaa acacctcgtc tgcatggtgt
     4021 ctgtgcaatc cacagtcgat aagctgtttg ggctctattg tcagccccag ctcttcctta
     4081 agctctctga gcgcagacgg tatgtagtcc acaccggccg gtatgtgtcc cgcgcttgat
     4141 atgtcgtagc agcccggaaa ggagtctttt tccatgcacc ttttctgcaa aagtatctgt
     4201 atggaatttc cacggcgtct gactatccag acatgcgcag tcctatgaag tatattgttt
     4261 ctgtgcgcct gcttgcgctc tacggtctgt cctgttggat ttccctgctc gtccactata
     4321 tcaaaatatt ccatatgaga ctcctttctt gtcg
//
LOCUS       DPUF01000262            7249 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_23447, whole
            genome shotgun sequence.
ACCESSION   DPUF01000262 DPUF01000000
VERSION     DPUF01000262.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_23447"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="DHV81_09090"
     CDS             complement(<1..1094)
                     /locus_tag="DHV81_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94583.1"
                     /translation="MKLPELKEKLKSKYIVRVVAGVLTIALVGTGIGATAVFAEKNST
                     AVTAEADSTTGSSKDADDIADKLMDSVSLKDNDADKDESVYLISDANGNVNKTIVVDH
                     LKNKDKKDTLEDASNLSDIENVKGKEKFTQSGDKLTWQAGGKDIYYQGTATEEPPVTQ
                     KVTYYLDGKEISPEDLAGKSGKVKIRFDYKNTTSYTETVNGEKQTVSVPFAAITGLVL
                     GDGFENIEVTNGKAEVSDSSSVVLGYALPGLKDSLGIKDKDLDGDVNIPEYMEMTADV
                     KNFSMPAAMTFVVNASDYVSTDGIDTSDLDDMINDLKDASTQLQDGSKTLAEGTDTLA
                     DGLSTLQSKLGTFASGVGTLQSGLKTYTDGV"
     gene            complement(1188..3300)
                     /locus_tag="DHV81_09095"
                     /pseudo
     CDS             complement(1188..3300)
                     /locus_tag="DHV81_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515988.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    1948..1976
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3571..4140)
                     /locus_tag="DHV81_09100"
     CDS             complement(3571..4140)
                     /locus_tag="DHV81_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCI94584.1"
                     /translation="MTQQGKNAIDLVLAESLIELSAKKPIDKITIKEITDKAGVIRPT
                     FYNHFQDKYELIEWIIKTELLEPIKPLLNAGMIFEAIVLMLNNITANSVFYKKALKLE
                     GAVTFNQIAMKCVYEMFMNIISEKSTGKKSKYRWLTPEVISTYYAQSLCFITEGWIAQ
                     GMDDIEPKEMAEAYMYLITRSMDDILSEL"
     gene            complement(4140..4361)
                     /locus_tag="DHV81_09105"
     CDS             complement(4140..4361)
                     /locus_tag="DHV81_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94585.1"
                     /translation="MEFKMERPGLTKVGDHLKVTESKLPTSYYYTIEHAIAMSGNYVV
                     SERLKSREGDVVDVRETEKGYFVTMEFDE"
     gene            complement(4393..4983)
                     /locus_tag="DHV81_09110"
     CDS             complement(4393..4983)
                     /locus_tag="DHV81_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94586.1"
                     /translation="MKEFLGRIKADFLLSSVLCIALGLVFIIWRASVLTIVANVLAVV
                     LIIIGVVYMCSYFLNIVTNGFSAMLGLVVLAVGIWFLVQPQVVVSLIPIVIGLVLIFH
                     SIRSIIEAVDARKYGYGGWSVGLIFSIISLIFGIICVTNAFDVMKTATVVVGIILIFN
                     GVSNIWITSRATKAESAYNKQKEFEATFVEDRKEDN"
     gene            complement(5023..5976)
                     /locus_tag="DHV81_09115"
     CDS             complement(5023..5976)
                     /locus_tag="DHV81_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94587.1"
                     /translation="MDKNKIKNKLKDDPAFTLEVQNPEVEALMTQYSSDKKAETLNKL
                     IEKCTKSRFLVPANVGENNKPIPLFIKNGEGEAFMPIYTSKAQLSKDHPSPCIVNMPF
                     LAVNNMVANKESKINGIAFNPFTHNLIFKKPLVEKIEAVEKARREGHPTSPTKGKTVQ
                     MTAEQYVIFERMQYEHIFLPAKMFEGGKEFMDRLADEKEKLIDELYEEAYQQKRMYPY
                     MEEDFSVMSMQISDTLNVTRVDFPTRDMAPNCAIRAYLVWDDAVSQGRYFVIDAKGDK
                     TTELAEITEDYKRLGYGAAPVEGAELQTVLDLVKKSAEEQN"
     gene            complement(6029..6523)
                     /locus_tag="DHV81_09120"
     CDS             complement(6029..6523)
                     /locus_tag="DHV81_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCI94588.1"
                     /translation="MRTLYTFDKHNYSDDMEVFERFAVRAVIIRDGKLAMQQAGEGYY
                     KILGGGIDAGETNEVALAREVREEAGLLVVPESIRECGKVIEKRRDIFEADKIYYCHT
                     YVYFCDVKDEHVAAQMTDSEIRLEYHLSWATPDEIIKANSAFLDKEWIARDTKIVELI
                     KEMC"
     gene            complement(6638..>7249)
                     /locus_tag="DHV81_09125"
     CDS             complement(6638..>7249)
                     /locus_tag="DHV81_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCI94589.1"
                     /translation="TSMVLGDRNITLYGKGFIEDKLCGCTFRISPGSFYQVNPVQTEL
                     LYEKAIHEAHLTGKERVIDAYCGTGTIGIIAAKNAGEVIGVELNRDAIRDAVTNAKRN
                     DIRNIRFYNDDAGKFMVEMAQNGEKADVVIMDPPRTGSDEAFLSSVVKLAPERVVYVS
                     CGPETLARDLKYLTKHGYTVKRATPYDCFPYTEHIETVVLLCR"
BASE COUNT         1757 a         1788 c         1382 g         2293 t
ORIGIN      
        1 acaccatcag tatatgtctt aagtccactc tgcagtgtgc caactcctga tgcgaatgtg
       61 ccgagcttgc tctgcaatgt tgaaagtccg tcagcaagtg tgtcagtacc ctctgccagt
      121 gtctttgagc catcctgaag ctgtgttgag gcatccttta agtcatttat catgtcatcg
      181 aggtctgatg tgtcaattcc gtctgtgctt acataatcag atgcgtttac cacgaaggtc
      241 atggctgcag gcattgagaa attctttaca tctgctgtca tttccatgta ttcagggata
      301 tttacatcgc catcgaggtc tttgtccttg attccgaggc tgtcctttaa gcccggaagt
      361 gcatagccga gcactacgct tgatgagtcg cttacctcag ccttgccgtt tgtcacttcg
      421 atattctcga agccatcacc gagtacaagt cctgttattg ctgcaaatgg tacggaaact
      481 gtctgcttct cgccgtttac tgtctctgta tatgatgtgg tgtttttgta gtcaaaacgg
      541 attttgacct ttcctgactt tcctgcaagg tcctcaggtg agatttcttt tccatcgagg
      601 tagtatgtaa ccttctgtgt tacaggtggt tcctctgttg ctgtgccctg atagtaaatg
      661 tccttgccgc cggcctgcca tgtgagctta tcaccgcttt gtgtgaattt ttccttgccc
      721 tttacgttct cgatatctga aaggtttgaa gcatcctcca aggtatcctt tttgtcttta
      781 tttttaaggt gatctactac gattgttttg tttacgttgc cgtttgcatc tgagatgagg
      841 tatacgctct cgtccttgtc tgcatcgtta tcctttaatg atacgctgtc catgagctta
      901 tctgctatgt catcagcatc cttgctgctt cctgttgtgc tatctgcctc ggctgtgact
      961 gcggtgctgt tcttctctgc aaaaaccgct gttgctccga ttcctgtacc tacaagtgct
     1021 attgtgagga ctccggctac aacacggaca atatatttac tttttaactt ttctttcaat
     1081 tctggtaatt tcatgtctta ttcctcctga ttttgttttt gaggtcatat ttttatgtta
     1141 tgagggtcaa aaagtatttt atccctcatt tatgattaat cagattacta agctgtcagg
     1201 ttcttgtttt taaagcctgc gcttgtggca catattactt tgtcaaaaat cataaacatt
     1261 gacggaagta taaatactac tacaaacata ctgatgattg cgcctcgtgc aagcagtata
     1321 cagatagaac tgatcatatc aatgtttgaa taaagtccta caccaaaggt tgcggcgaag
     1381 aaacagagtg cactgactat aactgagttt gtcgaatctg caagtgcctt tgtgattgcc
     1441 ggctttttgt ccattccggt gtatctgttc tttctgtact ttgtggtcat aagtatcgca
     1501 tagtcaaccg ttgcaccaag ctgtatggtt ccgattacga tgctcgcgat aaacggaagc
     1561 tttgttcctg taaatcccgg gattcccata tttacaaaga ttgcaaactc gatgaccgct
     1621 acaagtatga ttggaagcga tactgatttg aatactacga ggatgataag gaatatcaat
     1681 acgattgata ctgcacttac acgcttaaag tctgtatctg tgatagtgat aagatccttg
     1741 gtacaaggtg cctcaccgat aagcattgcg tccttgctgt aattctttac tatcttttta
     1801 atctctgtaa tctggttgtt tacctcatca gatgctgtct catattttga tgaaacaagc
     1861 atcatctggt aatcaccgtt tttgaatatc tcctttgcct catccggcac catatcatcc
     1921 ggtatggtag gtccgagtat tgagtcannn nnnnnnnnnn nnnnnnnnnn nnnnnngcga
     1981 taccgcaagc tttactccat cgaccttctt tatatcatct atcatgctta tgctgtctgc
     2041 ttttgacata tcgctcggcg cgatgatgat atgtgtggca cccatagcat actccttgtc
     2101 gagtctttca ttggcctgct tactgtatgc actgtcaggc atggttcctg caaggtcata
     2161 gtagacctga tcatgagtgt atccgtatat tgccgggatg agcactacga tgaaaatcac
     2221 tgcaagtatc ttataatgat ttgttaccca gcttgcaatc ctggaaacat ccggcataag
     2281 aatcttgtgc tttgtcttct cgattgcctt atcacatgca agaatcagtg cagggagaat
     2341 tgttacacat ccgataactc caaatacaac accctttgcc attacgattc ccaggtcaag
     2401 tccaagtgta aagctcatga agcagagggc tacgaagcct gcgattgttg tgattgagct
     2461 tcctgttact gagacaattg tgtgggaaat ggcatgtgcc atggctcgct tcttgtctcc
     2521 gtcatatctg tctatctgct cctgatagct gtgccacagg aaaatcgaat aatccattgt
     2581 tacggcgagc tgcaatactg ctgtcagtgc cagtgtcagg tatgaaatct gtccctgtat
     2641 aaagtttgtt cccaggttat agataatagc cattcctatg cttaggagga ataacatcgg
     2701 aacaattatt gaatccattg taattcccat gacaagcatt gaaagcaatg ctgctatgat
     2761 gacatatata ggagcctgct gcataacaag tgtctttgta tctgcgttga ctgctgccat
     2821 tcctgctacg aagcagtggt ttttcaccat cttatccatc ttgttgacgg cttccattgt
     2881 ctcatccgaa cccattgtgt cactgtaggt gacgagcatg agctggctgt ttgcatcctt
     2941 gttgttgaat gcgtcataca cctcatccgg aattgcctct cttggcaatg tggaatcagc
     3001 gattgttccg taccagatga catccttaac cccctcaagg tcctcaatgt catcagccag
     3061 agtctctatg tcctgatcct tcatatcctc taagatcaca aatgacaacg ctccggttcc
     3121 aaactcatct accatgatat cctggccctt catagtctct atctggcttg gcagataaga
     3181 aaggatatca aagttgattc tcgtgttgat aaaactgagg cccgacggta taagcagtgc
     3241 aaatgcaact atcagtatca gcacacgatg ttttacgact ccttttccaa atttaatcat
     3301 tagattcacc actctttctt ttattcgtat tgaagtataa aaaaaaagac cgaataaata
     3361 aatattcaat ctcttcgatt tgtcatgtga gatatacaaa tccatcaatt tgtatatcag
     3421 atatgacaca tatatgatat tgtatttgat ttctgacact ttttcacagc attttcatat
     3481 ttatatgaac gcatgcctct ttatggtgtc tctttatgat tctgctttat gagcttgctt
     3541 aagccgtcgt ttttccatgg caattgtcct ctacagctca cttagtatat catccattga
     3601 gcgcgtaatc agatacatgt atgcctccgc catctccttt ggctcaatat catccatccc
     3661 ctgggctatc cagccctccg tgataaagca caacgactgc gcataatatg tagaaatcac
     3721 ctccggtgtg agccagcggt atttggattt ctttcctgtg ctcttttcgc ttatgatatt
     3781 cataaacatc tcatagacgc acttcatggc aatctgatta aacgtcactg caccctccag
     3841 ctttagtgct ttcttgtaaa aaacactgtt tgccgtgata ttattgagca tcagcacaat
     3901 cgcttcaaaa atcattccgg cattgagtag cggcttgatg ggctcaagca gctcggtttt
     3961 gataatccac tctatcagct cgtacttgtc ctgaaaatga ttgtaaaacg tggggcggat
     4021 aactccggcc ttgtccgtta tctctttaat tgtaatctta tcgattggct ttttcgctga
     4081 aagctctata aggctctcag cgagcaccag atctattgca ttcttacctt gctgtgtcat
     4141 tactcatcaa actccatcgt gacaaagtag cccttctcag tctccctcac atccacgaca
     4201 tcgccctcgc ggcttttgag cctctcgctg accacgtagt ttcccgacat ggcaatcgca
     4261 tgctctatcg tataataata tgaagttgga agcttgctct ctgttacctt cagatggtca
     4321 ccgaccttgg taagtccggg tctctccatc ttaaactcca tccgacgcat tctgtcgcct
     4381 ttctgttact gcttaattgt cctcttttct atcctcaaca aacgttgcct caaactcctt
     4441 ttgcttgttg taggcgctct ccgcctttgt agcacggctt gtgatccaga tatttgatac
     4501 tccgttgaaa atcagtataa taccgacaac aacagtggct gtcttcatta catcaaatgc
     4561 atttgtcacg cagattattc caaaaatcag actgattatt gaaaaaatca ggccgacact
     4621 ccagcctccg taaccgtatt ttctggcatc caccgcctca ataatgctcc tgatgctgtg
     4681 aaaaatgagc accagaccga tgactatagg tatcaggctc acaacaacct gaggctgcac
     4741 cagaaaccat atgcccactg ccaaaaccac aagccccagc atcgctgaaa aaccattcgt
     4801 aactatattc aaaaaatagc tgcacatata gaccactccg atgatgataa gcactaccgc
     4861 aagcacattg gccacaatcg tgagcaccga agctctccat atgataaaca ccagtcccag
     4921 cgctatgcaa agcactgatg acagtagaaa atcagccttg attctgccta aaaactcctt
     4981 catacacgac ctcctttata tatcatgccc tacattactt ttctaattct gttcctctgc
     5041 acttttctta accaggtcaa gaacagtctg aagctcagca ccctcaaccg gtgcagctcc
     5101 atatccaaga cgcttgtaat cctctgtaat ctcagccagc tcagtagttt tatcgccctt
     5161 cgcatcaatt acaaaatatc tgccctgtga cacagcatca tcccacacga gataagcacg
     5221 gattgcgcag tttggagcca tatcccttgt cgggaaatca actctggtga cattaagcgt
     5281 atcagaaatc tgcatagaca taacagagaa atcctcctcc atatatggat acatacgctt
     5341 ctgctgatat gcctcctcgt acagctcatc tattaacttt tccttctcat cagcaagtcg
     5401 gtccatgaac tcttttccgc cctcaaacat cttcgccggc aggaaaatat gctcatactg
     5461 catacgctca aagatgacat actgctctgc tgtcatctgt acagtcttgc cctttgtcgg
     5521 tgatgtagga tgtccctctc ttctggcctt ttccactgcc tcaatctttt ctacaagagg
     5581 ctttttgaaa atcagattgt gagtgaacgg gttgaatgcg ataccattaa tctttgactc
     5641 tttatttgcc accatattgt tgactgcaag aaacggcata tttacaatgc atggtgacgg
     5701 atgatcctta gaaagctgtg cctttgatgt ataaatcggc atgaaagcct ctccctcacc
     5761 gtttttaata aatagcggaa tcggcttgtt gttctctccg acatttgcag gtaccaggaa
     5821 tctgctctta gtgcattttt caatgagctt attgagcgtc tccgcctttt tgtcgcttga
     5881 atactgtgtc atcagcgcct caacctccgg gttttgcacc tcaagagtga aggccggatc
     5941 atcctttaat ttatttttga ttttattttt atccatttat actgcttctc cttatattaa
     6001 atgcccggat ttacatccag gcattatttt aacacatttc ttttatcaac tcaacaatct
     6061 tagtgtctct tgctatccat tctttatcaa gaaaagcgct gttggccttt attatctcat
     6121 ccggcgtagc ccatgacaga tgatactcaa gccttatctc gctgtctgtc atctgtgctg
     6181 ccacatgctc gtccttcaca tcacagaaat acacgtaggt gtgacagtaa tatatcttgt
     6241 cagcctcaaa tatatcgcgt ctcttttcta tgaccttgcc acactccctg atgctttccg
     6301 gcacgacaag aagccctgcc tcctcgcgca cctcccgtgc cagcgccacc tcgttggtct
     6361 cgcctgcatc gatgccgccg cccagtatct tgtagtagcc ctcaccggcc tgctgcatgg
     6421 caagcttgcc gtctctgata ataaccgcgc gcaccgcgaa gcgctcaaat acctccatat
     6481 catcggaata attgtgtttg tcaaatgtat atagcgttct catgtttgtc ctccgtgtat
     6541 gggctgtaat cattcattag tatacatgga aagtagtttt tattctacac catttgacgg
     6601 cgaattggaa tactattatt gaccttttac ctgcctttta tctgcaaagt aagaccactg
     6661 tctctatgtg ctctgtatat ggaaagcagt catatggtgt tgcacgcttc actgtatatc
     6721 catgctttgt gagatacttt aaatccctcg ccagtgtttc cggaccacac gacacataca
     6781 ccactctctc cggtgccagc ttgacaaccg atgataaaaa tgcctcgtcg ctgccggttc
     6841 ttggaggatc cattatgaca acatccgctt tctcaccatt ttgtgccatc tcaaccatga
     6901 attttccggc atcgtcattg tagaagcgga tatttctgat gtcgtttcgc tttgcattgg
     6961 tgactgcatc acggattgca tcacggttta gctcgacccc gatgacttca cctgcatttt
     7021 tcgctgctat gatgcctatg gttccggtgc cgcaatatgc atcgattaca cgctccttgc
     7081 cggtgaggtg tgcctcatga atggcttttt cgtataaaag ctccgtctgc accggattca
     7141 cctgataaaa cgagcctggt gagatgcgga aggtgcagcc acacagctta tcctctatga
     7201 agcccttgcc ataaagcgtg atgtttctgt ctccgagcac catgcttgt
//
LOCUS       DPUF01000263            3317 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_3280, whole
            genome shotgun sequence.
ACCESSION   DPUF01000263 DPUF01000000
VERSION     DPUF01000263.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_3280"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="DHV81_09130"
     CDS             <1..798
                     /locus_tag="DHV81_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94590.1"
                     /translation="VIHDGGISLSVRNDASFIVDARLSIYEHQSTVCPNMPVRSLIYF
                     SVILSDMLSDKKRGTKSGKNIYGRRLVKIPTPHFVVFYNGEEEQPEVQELKLSDAFEK
                     PTDEPNLELKCKVYNINDGKNKAIMESCGWLNDYMTFVNKVREYHADGAFDDLAIDIE
                     KAIDYCIDNDILKEFLKTYRSEVTKSMQLNYEFDRQLELERADAIEEGMEIGIEKGIE
                     KGANKMLFTLVTKGKLDIDTAAEEAGVSVSEFEKLMSEAGYKVPETV"
     gene            complement(889..1173)
                     /locus_tag="DHV81_09135"
     CDS             complement(889..1173)
                     /locus_tag="DHV81_09135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94591.1"
                     /translation="MAILVWINVGVLSLIPLYIIDFKFFDKLNIGLQRKIALAKERGH
                     VVRGMIVKEDAKRLVVNNSQSAYSYWAARYTYTVDGKEYRKRFSCDNSRM"
     gene            complement(1187..1648)
                     /locus_tag="DHV81_09140"
     CDS             complement(1187..1648)
                     /locus_tag="DHV81_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HCI94592.1"
                     /translation="MTTDQKEKRNDEYNKYVKRVTPTHCLSANMLKAFILGGIICTIG
                     QLIVNTCTQYFGLSSDEASAWCSMILILISCILTALNLYAPLANWGGAGALVPITGFA
                     NGVCSSACEFQVEGQVFGIGCQIFRIAGPVILYGIFSSWVLGVIYLVVTSL"
     gene            1918..3181
                     /locus_tag="DHV81_09145"
                     /pseudo
     CDS             1918..3181
                     /locus_tag="DHV81_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741982.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; frameshifted; too many ambiguous
                     residues; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
     assembly_gap    2262..2341
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3237..>3317
                     /locus_tag="DHV81_09150"
     CDS             3237..>3317
                     /locus_tag="DHV81_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94593.1"
                     /translation="MIDLKFLRENPDLVKENIKKKFQDHKL"
BASE COUNT         1007 a          628 c          752 g          850 t
ORIGIN      
        1 gtgattcacg atggcggcat ttcactttcg gtcagaaatg atgcatcgtt tatcgtggat
       61 gcgagattga gcatatatga gcatcagtcc acagtttgtc cgaatatgcc ggtgagaagt
      121 cttatttatt tcagcgtgat attgtcagat atgctcagcg acaagaaaag aggaacaaaa
      181 agtggcaaga acatctatgg cagaagactg gtaaagatac cgacaccaca ttttgtggta
      241 ttttacaatg gtgaggagga acagccggag gttcaggagc ttaagctgtc cgatgcgttt
      301 gagaagccga ccgatgagcc gaatcttgag cttaaatgta aggtgtacaa tataaacgac
      361 ggaaagaaca aggctattat ggagtcctgt ggctggctga atgattatat gacatttgta
      421 aataaggtca gagagtatca tgcagacggg gcatttgatg atctggctat agatattgag
      481 aaggctatag actattgtat tgacaatgat attctgaagg agtttctaaa gacttacaga
      541 agtgaggtga cgaagagtat gcagttaaat tatgaatttg acaggcagct tgagcttgaa
      601 agagctgatg ctatcgagga agggatggaa ataggtatag aaaaaggtat agaaaaaggt
      661 gcaaataaga tgctgtttac tctggtaaca aagggcaagc tcgatattga tactgcagca
      721 gaggaagccg gtgtcagtgt cagtgagttt gaaaaattaa tgagtgaagc aggctataag
      781 gtgcctgaga ctgtgtagca tcaggcatga ataatttatc aaaatgtaaa tagcaaaaat
      841 ccgctaacca ttgtatttgg tatagcggat tttttgtgta ggaaagtatc acatccgact
      901 gttatcgcat gaaaaacgct tgcggtactc ctttccgtct acagtatatg tgtatctggc
      961 agcccagtag ctgtaagcgg attgtgaatt attgacaacg agcctctttg catcctcctt
     1021 cacaatcata cctcgcacca catgtccacg ctcctttgca agtgctatct tgcgctgtaa
     1081 accaatattt aatttgtcga aaaacttaaa atctatgata tacagtggta ttagtgaaag
     1141 cacacccaca tttatccaca caagaattgc cataaatgct aatcaatcac aagctcgtta
     1201 caacaaggta tatcacacca agcacccagc ttgaaaatat gccgtacagt atcacaggac
     1261 ctgcaatcct aaatatctga cagcctatgc caaatacctg tccctccacc tgaaattcac
     1321 atgcggatga gcatactccg tttgcaaagc ctgttatagg aacgagagca ccggcacctc
     1381 cccagtttgc gagtggtgca tacaggttta gcgctgtcag tatgcagctt atgagaatca
     1441 aaatcatgct gcaccaggca gaggcttcat cgctgcttaa cccaaagtat tgtgtgcagg
     1501 tatttactat gagctgtccg atagtgcata tgatgccacc gagtataaat gcctttagca
     1561 tgtttgcaga aaggcagtga gtaggtgtga cgcgctttac atatttgtta tattcatcat
     1621 ttcgtttttc tttttggtca gtagtcatgt tttcagacct ccttttttat aggatgaccg
     1681 tattttttaa atgtattcat atatgtgatt ttagttattt gatttggata tagttactta
     1741 ttagaattaa atatgaagga tatatcaaat aatccttgcc actttctgct ttaaatagta
     1801 gaattgtagc aaggattatt tttatgcggt agcgcatcaa agtaataaat cacagaaaag
     1861 catataattg cggtaaataa tgcaagtatt ttgcaataaa gaaaaggaga cacatatatg
     1921 tatacacttg acgacatcaa gaaagaagat caggagattg caagcgccat cacagacgaa
     1981 tttgagcgcc agaacagcca catcgagctt attgcttctg agaactgggt aagccctgct
     2041 gttatgtcag ctatgggaag catcctcacc aataagtacg ctgagggata tccgggaaga
     2101 agatactatg gaggctgtga gtgcgtggac gaggtagagg agcttgccag agaaagagcc
     2161 aagaagcttt tcggcgcaga gtatgtaaat gtacagccgc actcaggtgc tcaggccaac
     2221 atggcagtac agttcgccat cttaaagccg ggcgatacca tnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 ntcaggctca tatttccatg tagtgcctta tggagtaaat gacgagggct tcatcgatta
     2401 cgacaaggta gaggagatcg cccttgagtg caagccaaag atgattatcg caggtgcatc
     2461 tgcatatgcg agaactatag atttcaagcg tttcagagag attgcagaca aggtagacgc
     2521 agtgctcatg gttgatatgg ctcatatcgc agggcttgta gccgcaggac ttcatccaag
     2581 cccaatccca tatgcacatg tcaccacaac cactacacac aagactctcc gcggacctcg
     2641 aggaggaatg attctctgca gtcaggagat gcaggataag tacaatttca acaaggctat
     2701 tttcccggga atccagggtg gacctcttat gcacgttatt gctgcaaagg cagtctgctt
     2761 caaggaggca ttacagcctg agttcaagga gtatcagaag cagattgtaa agaacgcaca
     2821 ggcactttgc aaggggcttc agagccgtgg catcaagatt gtatcagacg gcacagacaa
     2881 ccacctcatg ctcgtagact taactccgtt tggtcttaca ggaaagtcta tcgagaagct
     2941 tctcgatgcg gctcatatca cagccaacaa gaatactatt cctaacgacc cacagaagcc
     3001 atttgtcaca agcggtatac gtctcggaac acctgctgcc acatcaagag gcttaaagga
     3061 ggatgatttc gacaaggttg cagaggcaat cgccctgatc atcaaagagg gcgagagtgc
     3121 agttgagcct gcaaaggcta tcatcaagac acttactgac aagtatcctc ttgcatttta
     3181 agggttttca gagtaaaatg ttataagtta taaaaacata atactttgga ggaattatga
     3241 tagacttaaa gtttttaaga gagaatccgg atctcgtaaa agaaaatatt aaaaagaaat
     3301 tccaggacca caagctt
//
LOCUS       DPUF01000264            2928 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12095, whole
            genome shotgun sequence.
ACCESSION   DPUF01000264 DPUF01000000
VERSION     DPUF01000264.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12095"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="DHV81_09155"
     CDS             complement(<1..521)
                     /locus_tag="DHV81_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888000.1"
                     /note="functions in MreBCD complex in some organisms;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HCI94594.1"
                     /translation="MVGSDIGIDLGTASILVYIKGKGVVLKEPSVVAFDRDTNKIKAI
                     GEEARLMLGRTPGNIVAVRPLRQGVISDYTVTEKMIKHFIQKALGKKSFRKPLVSVCV
                     PSGVTEVEKKAVEDATFAAGARDVKIIEEPIAAAIGAGIDISKPCGNMIVDIGGGTSD
                     IAVISLGGSVVSTS"
     gene            complement(637..>2928)
                     /gene="uvrA"
                     /locus_tag="DHV81_09160"
     CDS             complement(637..>2928)
                     /gene="uvrA"
                     /locus_tag="DHV81_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743545.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HCI94595.1"
                     /translation="MYELSEDIPMEKNIKHNIDIVVDRLVVKPGIEKRLTDSLENVFE
                     LTEGNAIVDIVDGEQMTFSQNFACPDCGISIDEVEPRSFSFNNPFGACPTCYGLGYKM
                     EFDPQLMVPDSSLSISEGAIQVMGWQSCTDPKSYTYATLRALHEGYGLPLDTPIDDLS
                     ADMKKLLFYGGDGRVLKVRYKGQRGEGVYDLVWNGLVDNVERRYKETFSDTAKAEYEQ
                     FMRITPCTSCKGQRLKPSSLAVTVADKNIYEMTSMSVKELVKFLADIELTEQQHYIGD
                     QILKEIRARVGFLQQVGLDYLTLTRGTASLSGGEAQRIRLATQIGSGLVGVAYILDEP
                     SIGLHQRDNDKLLGALMNLKNLGNTLIVVEHDEDTMRAADYIVDVGPGAGSHGGEIVA
                     AGSLKDIIRCKKSLTGAYLSGKIKIPVPQERRKPSGYITIRGARENNLKNIDVQIPLG
                     IMTCVTGVSGSGKSSLTNEILYKHLAKSLNRARCIAGDHDGIDGLEQLDKVIDIDQSP
                     IGRTPRSNPATYTGVFDMIRDLFASTPDAKAKGYKKGRFSFNVKGGRCEACGGDGILK
                     IEMHFLPDVYVPCEVCGGKRYNRETLDVKYKGKSIYDVLDMTVEEALEFFKNVPKIQN
                     KIQSLYDVGLSYVKLGQPSTELSGGEAQRIKLATELSRKSTGKTIYILDEPTTGLHFA
                     DVHKLVEILRRLSDGGNTVVVIEHNLDVIKTADYIIDMGPEGGDGGGTVIAQGTPEEI
                     CEVKQSYTGRFLKPYLEKDKDKL"
BASE COUNT          688 a          805 c          578 g          857 t
ORIGIN      
        1 gatgtgctga ctaccgaacc acctagtgag ataactgcta tatctgatgt accgcctccg
       61 atatctacta tcatatttcc gcacggcttt gaaatatcaa tacctgcacc gattgcagct
      121 gcgattggct cctcgataat cttaacatct ctggctcctg ctgcaaatgt agcatcctca
      181 acggctttct tctcaacctc tgtaacacca cttggcacac atacgctcac gagcggcttt
      241 ctgaagctct tcttaccaag cgccttctgg atgaagtgct tgatcatctt ctctgtaact
      301 gtataatctg agataacacc ctgacgcagc ggtctgactg caacgatatt acccggtgtc
      361 cttccgagca tgaggcgtgc ttcctctccg attgccttaa tcttgttggt gtccctgtca
      421 aaggctacta ctgaaggctc ctttaacaca acaccctttc cttttatata aacaagtata
      481 cttgctgttc ctaaatcgat tccgatatct gatccgacca ttttaatagc ccctttcctg
      541 ccatatcttt aaatttacac atacactctc ttattatata caataaatcc catttttcaa
      601 actttttttt tgaaaaaggt catatttaat aaaattttaa agtttatcct tgtctttttc
      661 cagatatggt ttcaaaaacc ggcctgtata tgactgtttt acctcgcata tctcctcagg
      721 tgtgccctgc gctatgacgg ttccgccgcc gtctccgccc tcaggtccca tatctattat
      781 atagtcagct gtctttatca cgtcgaggtt gtgctcgata acaactacag tattgcctcc
      841 gtctgagagc ctgcgcagta tctccacaag cttgtgtaca tctgcaaagt gcagtcctgt
      901 ggtaggctca tcaagtatat atatggtctt tccggtgctc tttctgctaa gctctgtggc
      961 aagcttgata cgctgtgcct caccacccga aagctctgtg gacggctgtc ccagcttcac
     1021 gtaggatagt ccgacatcat agagcgattg gattttgttc tggatctttg gcacattttt
     1081 gaaaaactca agtgcctcct ccactgtcat atcaagaaca tcgtatatgc tcttgccctt
     1141 gtactttaca tcgagtgtct ccctgttata tcgtttgcct ccacagacct cacacggcac
     1201 atatacatca ggcaggaaat gcatctctat cttaaggatt ccatcgccgc cacaggcctc
     1261 acaccggccg ccctttacat tgaaactgaa acggcccttt ttatagccct ttgccttggc
     1321 atcgggtgtt gatgcaaaca ggtctcttat catatcaaac acgcctgtat atgtggcagg
     1381 attagagcgg ggtgtacggc ctatcggtga ctggtcgatg tctatgacct tatcaagctg
     1441 ttcaagacca tctataccgt cgtggtcacc tgcgatacac ctggctctgt ttaaggattt
     1501 ggccagatgc ttgtatagta tctcattggt caatgagctc tttcccgagc ccgacacgcc
     1561 tgtaacacag gtcatgatac caagagggat ctgcacatca atatttttca ggttattctc
     1621 cctggctcct cttatcgtga tatatccgct tggcttgcgc ctctcctgtg gcaccggtat
     1681 ctttatttta ccgctcagat atgctcctgt gagtgatttt ttgcacctta taatgtcctt
     1741 taacgagcct gctgccacta tctccccacc gtgcgagccg gctcccggcc ctacatccac
     1801 gatataatcc gctgcacgca tggtatcctc atcatgctcc acaactatga gcgtatttcc
     1861 cagattctta agattcataa gtgcaccaag cagcttgtcg ttatccctct gatgcagtcc
     1921 gatacttggc tcatcaagta tatatgccac acctacaagt cctgagccaa tctgcgtggc
     1981 aagcctgatt cgctgggctt ccccgcctga aaggctcgcc gtgccacggg taagagtgag
     2041 gtaatcaaga ccgacctgct gcaaaaagcc aactctcgca cggatttcct ttagtatctg
     2101 gtctcctata tagtgttgct gctctgtaag ctctatatca gcaaggaact tcacaagctc
     2161 cttcacagac atggaggtca tctcatatat gttcttgtcc gcaaccgtaa cggcaagtga
     2221 cgacggctta agcctctgtc ccttacagct cgtacacgga gtaatgcgca taaactgctc
     2281 atactctgcc tttgccgtat cagaaaaggt ctccttgtat cggcgctcaa cattgtccac
     2341 aaggccgttc catacaaggt catagacgcc ttcgcctctc tgacccttgt atctgacctt
     2401 gagcaccctg ccatctccac cgtaaaacag cagttttttc atatctgcag ataaatcatc
     2461 aatcggtgtg tcgagaggaa gcccatagcc ctcatgcagc gcacgaagtg tcgcataggt
     2521 atagctcttt gggtctgtac atgactgcca gcccatcact tgtatggcgc cctcagatat
     2581 ggaaagcgat gagtcaggca ccataagctg cgggtcaaac tccatcttgt agccgagtcc
     2641 gtagcaggtc ggacatgctc cgaacggatt gttgaaggag aagcttctcg gctcaacctc
     2701 atctatgctt atgccacagt cagggcacgc aaagttctgg gaaaaggtca tctgttcgcc
     2761 atccacaata tcaactatgg cattgccctc tgtcagttca aatacatttt caagtgaatc
     2821 agtaaggcgc ttctctatac caggctttac aaccagacga tccactacta tatcaatatt
     2881 gtgctttata tttttctcca tcgggatatc ctcggagagc tcatacat
//
LOCUS       DPUF01000265            2701 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_48939, whole
            genome shotgun sequence.
ACCESSION   DPUF01000265 DPUF01000000
VERSION     DPUF01000265.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_48939"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            99..851
                     /locus_tag="DHV81_09165"
     CDS             99..851
                     /locus_tag="DHV81_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94596.1"
                     /translation="MKRFVTYLYLYDGRIKGNNAGFIKVEINGEKSAFLVQIKNLGKY
                     SGKGKVYLVYEAEAVIEAGELTVKNGEAREIYNVDTENVKDSGKTFLQVLGVRIELEN
                     ADEKFLASCWTDNTEAVIGKPLKVQADLSNKKKPVSTPMPEPGCQKEIHSQNVDDXXX
                     RIDISELRTLPAKNWYIVNNSFLTHGYANYKHLVIKTDTDGRQYLGVPGVSEPQERML
                     AGIFGFTEFEPAAPANAADVDSGVFGYWFCPL"
     gene            952..1734
                     /gene="udp"
                     /locus_tag="DHV81_09170"
     CDS             952..1734
                     /gene="udp"
                     /locus_tag="DHV81_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCI94597.1"
                     /translation="MKNYSKDPDRQYHIQVAKGEVGRYVIMPGDPKRCVKIAQYLDNP
                     VLIADNREFITYTGTLDGVKVSVTSTGIGGPSASIAMEELYRCGADTFVRIGTCGGMQ
                     TDVKSGDIVIATGAIRMEGTSKEYAPIEFPAVADLDVTNALVAAAKQKNYTHHTGVVQ
                     CKDAFYGQHEPEKMPAGYELINKWNAWRMLGCLASEMESAALFVVAAKLGVRAGSCFL
                     VVANQEREKLGLENPVVRDTDMAVQVAVSAVRNLIREDCAVE"
     gene            1769..2422
                     /gene="deoC"
                     /locus_tag="DHV81_09175"
     CDS             1769..2422
                     /gene="deoC"
                     /locus_tag="DHV81_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCI94598.1"
                     /translation="MDINEILKHVDHTLLSPQATWVEIRKICDDAIKYHTASVCIPPS
                     YVSQAAKYLGERVKVCTVIGFPNGYSTTAVKAFETEDALANGAKEIDMVINIGWLKDK
                     RYELIEDEIKKLKSICKDKVLKVIIETCFLTDDEKIRMCDIVTSAGADYIKTSTGFGT
                     AGATFDDIKLFSEHIGEGVKMKAAGGISSLDDAERFLELGADRLGTSRVVKLIKAMD"
     gene            2458..>2701
                     /locus_tag="DHV81_09180"
     CDS             2458..>2701
                     /locus_tag="DHV81_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCI94599.1"
                     /translation="MDKKTIEKLIDTALAQLDFSYAPYSGFNVGAALLAKNQAVYTGC
                     NIENAAYTPTNCAERTAFFKAVSEGVRDFEAICIVGG"
BASE COUNT          854 a          519 c          721 g          599 t
ORIGIN      
        1 ggctcgaata gctataaatc aagtataaat taataataca cataatatca ctgctccact
       61 catagaatag tacaaaacgc gcaggcgggt aaataagcat gaagagattc gttacatacc
      121 tgtatttata tgatggcaga ataaaaggaa ataatgcagg ctttataaaa gtagaaataa
      181 atggagaaaa aagtgcattt ctcgtacaga taaaaaatct cggaaaatac tccggaaaag
      241 gaaaagtgta tctggtatat gaagcggagg cggtcataga ggcaggagag cttacggtaa
      301 aaaacggtga agcgcgagag atatataatg tagataccga aaacgtaaag gacagtggaa
      361 agacattttt gcaggtgctc ggtgtaagga tagaactgga aaatgcagat gaaaaatttc
      421 tggcaagctg ctggacggat aacacggaag cagttatagg aaaaccactc aaggtacaag
      481 cagatttatc aaataagaaa aaacctgtat cgactcctat gcctgaaccg ggctgtcaga
      541 aagaaatcca tagtcaaaat gttgatgacn nnnnnnngcg catagacata tcagagcttc
      601 gcacgcttcc ggccaaaaac tggtacatag tcaacaacag ctttcttaca cacggttacg
      661 caaactataa gcatctcgtc atcaaaacgg atacagatgg caggcaatat ctcggagtgc
      721 cgggcgtgag cgagccgcag gagcgcatgc tggcagggat attcggcttc acggaatttg
      781 agccggccgc cccggcaaat gcagcggatg tcgatagcgg agtgttcgga tactggttct
      841 gcccgctgta gtttttatag attgtaagaa aatgtccttt cctctgcaca aaaccacaga
      901 aattgtgtat aattaaagct aacaaaagaa aactgtggga ggtggcatta catgaaaaat
      961 tattcaaagg atcctgacag acagtatcat atccaggtcg caaagggaga ggtcggcagg
     1021 tatgttatca tgccgggaga cccgaagaga tgtgttaaaa tagctcagta ccttgacaat
     1081 ccggtcttga tagctgacaa cagggagttt atcacataca ccggcacatt ggatggagtg
     1141 aaggtcagcg taacatcgac aggcatcggc ggaccatccg catccatcgc gatggaggag
     1201 ctgtacagat gcggtgcaga taccttcgtg aggatcggaa cctgcggagg catgcagaca
     1261 gatgtgaaaa gcggtgacat cgtgattgcg acaggcgcca tccgcatgga gggcacatca
     1321 aaggagtatg cgccgataga gtttccggct gtggcagatt tagatgtcac aaacgcgctt
     1381 gttgcggcag caaagcagaa aaactatacg catcacacag gcgtggtgca gtgcaaggat
     1441 gcattttacg gacagcacga gcctgaaaaa atgccggcag gctacgagct tatcaataaa
     1501 tggaatgcat ggaggatgct tggctgtctt gcgtcagaga tggagtcagc cgcgcttttt
     1561 gtcgttgcgg caaagcttgg agtcagggca ggctcatgct tccttgtggt tgcaaatcag
     1621 gagagggaaa agctcgggct tgagaatcct gtggtacgcg atacggacat ggcggttcag
     1681 gtggcagttt cggcagtgcg aaacctcatt cgtgaggatt gtgccgtaga ataagtcaga
     1741 cataaaacac agaaacggag aacagacatt ggatataaat gaaattttaa aacacgttga
     1801 ccatacactt ttatccccac aggctacatg ggtcgaaatc aggaagatat gcgatgatgc
     1861 gataaaatat cacacagcat cggtctgcat accgccaagc tatgtaagtc aggcagcaaa
     1921 gtacctgggt gaaagggtaa aggtctgtac agtgataggc ttccctaacg gatattcgac
     1981 cactgcggtg aaagcctttg agacagagga tgccctcgca aacggagcga aggagataga
     2041 tatggtcatc aatatcggct ggctcaagga taaaaggtat gagctgatag aggatgaaat
     2101 aaagaagctc aagtccatat gcaaggataa ggtgcttaaa gtcatcatcg agacctgctt
     2161 tctcacagat gatgagaaaa tcaggatgtg cgatatcgtg acgagtgcag gggctgatta
     2221 catcaagaca tctacaggct ttggcacggc aggtgcgacc tttgacgata taaagctgtt
     2281 ttcagagcat atcggagagg gtgtgaagat gaaggctgcc ggaggcattt cttcattaga
     2341 tgatgcagaa agatttcttg agcttggcgc tgacaggctt gggacaagcc gtgtggtgaa
     2401 gctcattaag gctatggact gaattttcgt atacaataaa taaccgggga attatatatg
     2461 gataaaaaaa caattgaaaa attgattgat acagcccttg cacagcttga cttttcatat
     2521 gcgccgtact caggcttcaa tgtaggtgcg gctcttttag caaaaaatca ggcagtctac
     2581 accggctgca atatagaaaa tgcagcctac acaccgacaa actgtgcgga aaggacagca
     2641 tttttcaagg cggtgagtga aggcgtgcgg gattttgagg caatctgtat cgtgggcgga
     2701 a
//
LOCUS       DPUF01000266            4025 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_30387, whole
            genome shotgun sequence.
ACCESSION   DPUF01000266 DPUF01000000
VERSION     DPUF01000266.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_30387"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="DHV81_09185"
     CDS             <1..252
                     /locus_tag="DHV81_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI94600.1"
                     /translation="KTAAKVLTLTDKIIKENSLTAMMVTHNMRDAIAHGNRLIMMNNG
                     QVVLNISGEEKKNLTVEDLLMKFEEVSGEEFASDKALLG"
     gene            complement(323..988)
                     /locus_tag="DHV81_09190"
     CDS             complement(323..988)
                     /locus_tag="DHV81_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HCI94601.1"
                     /translation="MDNFVVTFARGFGTGGKAIASQLAKELGIHCYENRILTLASQLS
                     GLDENVFQEVNEKLREEGGFTSFLRGLPRAKGYISRNEKFKSDDRLFEYQSQIINELA
                     DKESCVIVGKCADYVLKNRPNVVSIYIEAPRAFCVERTIANMGVTSEVANATIERTDK
                     FRADYYKYYTHGNYWTNPVNYDLTLNSERVGVENCVKTIEQYLIIKGFIKDDMIKPAG
                     ELI"
     gene            complement(1022..1780)
                     /locus_tag="DHV81_09195"
     CDS             complement(1022..1780)
                     /locus_tag="DHV81_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI94602.1"
                     /translation="MNRRRNMSETVISIKDLSKSFGDHEVLRKIDIDVHSGEVICIVG
                     SSGSGKSTLLRCINKLEKQTSGKILYHDKEVRNVQKDINDYRSKVGMVFQSFNLFNNM
                     TVLQNCMLCTRRVLHLPKQEAFDRAITHLKAVGMAPYINAKPSQLSGGQKQRVAIARS
                     LCMNPEVLLFDEPTSALDPEMVGEVLNVMKDLARTGLTMIIVTHEMAFARDVSTRTIF
                     MDSGYVAEDAAPSELFTNPKNPRTREFLSRYLAG"
     gene            complement(1773..2528)
                     /locus_tag="DHV81_09200"
     CDS             complement(1773..2528)
                     /locus_tag="DHV81_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCI94603.1"
                     /translation="MISNPSNSLEWMVFLAQKYSSMFIKGTWLTLYISVVGTLLGFVL
                     GYIVGIVNDVKLNDGDNIVKKVLIRIVKVIFTVYVEVFRDTPMIVQAMIVYYGIRQMG
                     YENLTPVFAGILVTVLNTGAYMGETVRAGIGSIDMGQREGAWAMGMSPFKTMLYVVLP
                     QAFKNIIPEMANTFLLNLKMTSVLNVIAVQELFMAAKTVGGNYYKYFESYLVIAVIYF
                     VLCFVFDKLFKLIEKKLQGKTDYALAVEYMDNE"
     gene            complement(2660..3661)
                     /locus_tag="DHV81_09205"
     CDS             complement(2660..3661)
                     /locus_tag="DHV81_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI94604.1"
                     /translation="MQSCFIKFIIRTKQRRWRKTPAPFRISIIKEEKIMMKRSKRFAA
                     LILTGVMAATTLFGCGSSSGGAASDSSAADSSSKKVLKVGMECAYAPFNWTQDTDTTP
                     DGSKAVKIAGSDYYAYGYDVAVAQKLADQMGMDLEVHKVEWSSIGISLDAGDYDCIIA
                     GMGKTKEREASYAFTEPYYYRNNCIVVKKGGKYSNVKSLSDLADTGCKVTTQLGTGWI
                     PLLDQIKGAEQSGNFETTSECFLAISNGSADVCVIDVPTAESAALTNDDLQIIELDEN
                     DTFTGDDEMVNVCIATRKDDTALRDKIQDAMNAIGWNDKAKMDELMDQVLTQQPAAN"
     gene            complement(3702..>4025)
                     /locus_tag="DHV81_09210"
     CDS             complement(3702..>4025)
                     /locus_tag="DHV81_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI94605.1"
                     /translation="ISASSMGIYKYMFNSGNQQEIMDYCNEKLKRLEMYDNANGSFLI
                     DTLLNYYMCGFNVQKTAQMMYVHRNSLQYRLKKIEEILEISLDDSMEYLDVVNCILVK
                     RLMFN"
BASE COUNT         1037 a         1001 c          729 g         1258 t
ORIGIN      
        1 aagacagccg caaaggtgct cactctcaca gacaaaatca tcaaggagaa cagcctgaca
       61 gccatgatgg tcacacacaa catgcgtgat gccatcgctc acggcaacag actcatcatg
      121 atgaataacg gacaggttgt ccttaatatc tcaggcgagg aaaagaaaaa cctcactgtg
      181 gaggatctgc tcatgaagtt tgaggaggtt tccggcgagg aatttgcttc tgataaggct
      241 ttgcttggat agacatatct atataataat aacaaaaatt tgcagctcaa tacacgagct
      301 gcaatttttt gttatatact ttttatatga gctctcccgc cggtttaatc atatcatcct
      361 tgataaagcc ctttatgatc agatactgct ctatcgtctt cacacagttt tccacaccaa
      421 cacgctcact atttagtgtc aggtcgtagt tcaccggatt ggtccagtag tttccgtgtg
      481 tatagtactt gtaatagtct gccctgaact tatctgttct ctcaatcgtg gcatttgcca
      541 cctccgatgt cacacccata ttggctatgg tacgctccac acagaatgca cgcggtgcct
      601 caatgtatat gctcactaca tttgggcggt tctttaatac atagtcggca cattttccta
      661 ctatgacgca ggattccttg tctgcaagct cgttgataat ctggctctga tactcaaaga
      721 gcctgtcatc tgatttgaac ttctcatttc ttgatatata tccctttgct cttggaagcc
      781 ctcgcaaaaa gctcgtaaat ccgccctctt cacgcagctt ctcatttacc tcctgaaaca
      841 cattctcgtc aagtccgctc agctggcttg caagagtcag aatacggttc tcataacagt
      901 gtattccaag ctcttttgcc agctgtgatg ctattgcttt tccgcctgtt ccaaatcctc
      961 tggcaaaggt tactacaaaa ttatccattt atcatttcct ccttctgaat gccaaccata
     1021 tctagcctgc cagatatctg cttaagaatt ctcttgttct tggattctta ggatttgtaa
     1081 acagctctga cggtgctgca tcctctgcca catatcctga gtccatgaaa atagttcttg
     1141 tagaaacatc tctggcaaat gccatctcgt gtgtgacgat tatcatcgta agtccggttc
     1201 ttgcgaggtc cttcatgaca tttagcacct ctccgaccat ctcagggtca agcgccgagg
     1261 tcggctcatc aaacaaaagc acctccgggt tcatacagag cgacctggca atggcaactc
     1321 gctgcttctg tcctcctgag agctgcgatg gctttgcatt gatataagga gccatgccga
     1381 ctgccttcag atgcgtgatt gccctgtcaa aggcctcctg ctttggcaga tgaagcaccc
     1441 ttcttgtaca aagcatacag ttctggagca ctgtcatatt gttgaacaaa ttgaaggact
     1501 ggaacaccat tcccaccttt gaacggtaat cattgatatc cttttgtaca tttctgacct
     1561 ctttatcgtg atataaaatc tttcctgagg tctgtttctc aagcttgttg atgcacctaa
     1621 gcagtgttga ttttcccgag cctgaggagc ccacaataca gatgacctcg ccggaatgca
     1681 catcaatatc aatctttcta agtacctcat gatcaccaaa tgatttgctt aaatccttaa
     1741 ttgaaattac tgtctcactc atattgcgcc tcctattcat tgtccatgta ctcaactgcg
     1801 agtgcatagt ctgtcttgcc ctgcagcttc ttttcaatca gcttgaagag cttatcaaat
     1861 acaaagcaaa gtacaaagta aattaccgca atgaccagat atgactcaaa gtacttgtag
     1921 tagtttccac cgaccgtctt ggcagccatg aaaagctcct gcacagcgat aacattgagc
     1981 actgatgtca tcttcagatt taaaagaaat gtatttgcca tctcaggaat gatgttcttg
     2041 aatgcctgcg gcaaaactac atagagcatt gtcttaaacg gtgacattcc catggcccat
     2101 gcgccctcgc gctgtcccat atctattgaa ccgattcctg ctctgactgt ctctcccatg
     2161 taggcacctg tatttaatac agttacgaga atacctgcaa atacaggtgt caggttttca
     2221 tagcccatct gacgtattcc atagtataca atcatcgcct gaactatcat tggtgtgtcc
     2281 ctgaatacct caacataaac tgtaaatatc acctttacta tacggataag gacctttttt
     2341 acgatgttgt cgccatcgtt aagctttaca tcatttacta tgcctacgat atatcccaat
     2401 acgaagccaa gcaatgtacc aactaccgat atgtacagcg taagccaggt tcctttgata
     2461 aacatgcttg aatatttctg cgccagaaat accatccatt ctaacgaatt acttggatta
     2521 cttatcatca tactgctacc tcacttttat ctctctaatc cccagcttac gccggtttat
     2581 cagactattg tgggatgatg cgatataaca tcatcccaca gtcctttatc ttttcgtgaa
     2641 tcagataaat ctctaacagc tagttggctg ccggctgctg tgtcagaacc tgatccatca
     2701 gctcatccat ctttgccttg tcattccagc cgattgcatt catggcatcc tggattttat
     2761 ctctgagtgc tgtatcatcc tttcttgttg cgatacatac atttaccatt tcatcatcac
     2821 ctgtgaatgt atcattctca tccagctcga taatctgtaa atcatcgttt gtcagtgctg
     2881 ctgactctgc tgtcggaaca tcgattacac atacatctgc gctgccgttt gatattgcga
     2941 ggaagcactc tgatgtagtc tcaaagtttc ctgactgctc agcacccttt atctggtcta
     3001 aaagtggaat ccagcctgtt ccaagctgtg ttgttacttt gcagcctgta tctgcaaggt
     3061 ctgaaaggct ctttacattt gaatacttgc cacccttctt aacaacgata cagttgtttc
     3121 tataataata aggctctgtg aatgcgtatg atgcttctct ttcctttgtc ttgcccatac
     3181 ctgcgatgat acagtcataa tctcctgcat cgagtgaaat accgattgat gaccactcaa
     3241 ccttatgtac ctcaaggtcc attcccatct ggtctgcaag cttttgtgcc actgctacat
     3301 catagccgta tgcatagtaa tcagagcctg ctatcttgac tgccttgctg ccgtccggtg
     3361 tggtatctgt atcctgtgtc cagttgaatg gagcatatgc acattccata ccaaccttta
     3421 aaaccttctt tgatgagctg tctgccgctg agctatctga agcagctcct cctgatgacg
     3481 aaccacatcc aaacaatgtt gtcgctgcca ttacacctgt caatatcaat gctgcaaatc
     3541 tcttacttct cttcatcata atcttttcct ccttgattat tgaaatacga aaaggcgccg
     3601 gagtttttct ccagcgcctt tgcttagttc gtataataaa cttaatgaag cagctttgca
     3661 atgttaattt tgcacaattt gtttgtgcat ttttacagta actaattaaa cataagcctt
     3721 tttacaagaa tacaattaac tacatccaga tattccatcg agtcatcaag gctgatttcc
     3781 agtatttctt ctatcttttt cagacggtat tgcagggaat tgcgatgaac gtacatcatc
     3841 tgtgccgttt tttggacatt aaagccacac atataataat tgagcagcgt atctatgaga
     3901 aacgagccgt ttgcattgtc atacatctca agccgcttta gcttctcgtt gcaataatcc
     3961 atgatttcct gctgattgcc gctgttgaac atatacttgt atatgcccat ggagcttgca
     4021 ctgat
//
LOCUS       DPUF01000267            5346 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_484, whole
            genome shotgun sequence.
ACCESSION   DPUF01000267 DPUF01000000
VERSION     DPUF01000267.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_484"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            402..2006
                     /locus_tag="DHV81_09215"
     CDS             402..2006
                     /locus_tag="DHV81_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCI94606.1"
                     /translation="MPVKYVFVTGGVVSGLGKGITAASLGRLLKARGYKVTMQKFDPY
                     INIDPGTMNPIQHGEVFVTDDGAETDLDLGHYERFIDESLTKNSNVTTGKVYWSVLQK
                     ERRGDYGGGTVQVIPHITNEIKSRFYRDFTSEDTTIAIIEVGGTVGDIESQPFLEAIR
                     QFQHEVGHDNAILCHVTLIPYIKASGELKTKPTQASVKELQGMGIQPDIIVCRSEHEL
                     DQKLKDKIALFCNVPNSHVLQNLDVEYLYEAPLAMEQENLAKVACECLNLDCPEPDLA
                     DWKQMVEDLRHPDKEVKIALVGKYTALHDAYISVVEALKHGGIPEHTTVDIKWVDSEE
                     LNDDNAAEVFKGIQGIIVPGGFGDRGVEGMIAAAKYARENNIPYLGLCLGMQVAIIEY
                     ARHVCMFHDAHSIELDPNTTHPVIALMPDQNGIEDIGGTLRLGSYPCVLDKTSKAYQV
                     YGTENISERHRHRYEVNNDYRTALTEHGMKLCGTSPDGRIVEMIEIPEHPWFIATQAH
                     PELKSRPNRPHPLFHGFIEAANKVNR"
     gene            2192..4284
                     /locus_tag="DHV81_09220"
                     /pseudo
     CDS             2192..4284
                     /locus_tag="DHV81_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741594.1"
                     /note="frameshifted; too many ambiguous residues; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase HflB"
     assembly_gap    3604..3615
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3948..3990
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4628..>5346
                     /gene="uvrC"
                     /locus_tag="DHV81_09225"
     CDS             4628..>5346
                     /gene="uvrC"
                     /locus_tag="DHV81_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HCI94607.1"
                     /translation="MEKTFDIKEELKKLPDAPGVYIMHDKDDVIIYIGKAKSLTKRVH
                     QYFQASHNEGIKKKQMVEHIDHFEYIVTDSELEALVLECNLIKEHTPKYNTMLRDDKT
                     YPYIKVTLGEDYPRVLFSRQMKKDKSRYFGPYTSASAVKSSIDLINKIYKLRTCNRRL
                     PRDIGADRPCLNYHIHQCSAPCQGYVTKEEYAISVKGAIDFLNGDYEQTLKALSDKML
                     KASESMEFEKAAEYRDLINSV"
BASE COUNT         1685 a         1048 c         1325 g         1233 t
ORIGIN      
        1 gcacagattg attacaaata tgacgagact gtcaatcagt ttaagtagat tttcagcata
       61 catattaaaa gtgatggaag gataagataa tggagacatg ctgcgatttt tgtgcataca
      121 atgaatatga tgaggatgat gaggagtatt attgctcggt aaatatggac gaggatgata
      181 tggcgagatt tgtccagagc agctataagc gctgtccgtt ttaccgctca ggcgatgaat
      241 acagggttgt ccgtcaccag atgtgaagca ttataacaaa atataataaa tataattaaa
      301 aaataaattg cattaatcaa tttaaggctg tataataagt tggttatttt aaaaattaag
      361 aatttacaaa tatacaaagc atattgataa ggaggaaaat tatgcctgta aaatacgtgt
      421 tcgtcaccgg tggagttgtt tccggtcttg gaaaaggaat taccgcagcg tctcttggac
      481 gtcttttaaa agctcgtgga tacaaggtga caatgcagaa gttcgatcca tacattaata
      541 ttgatccggg taccatgaac cctattcagc acggagaggt gtttgtaaca gacgatggag
      601 ctgagacaga tcttgacctg ggacactatg agcgttttat tgatgagagc ctgacaaaga
      661 actcaaacgt caccacagga aaggtatact ggtcagtatt acagaaggag cgtcgtggtg
      721 actacggcgg aggaacagta caggttatcc ctcatatcac caatgagata aagagcagat
      781 tctacaggga tttcacatct gaggatacca caatcgcaat catcgaggtg ggcggtacag
      841 tcggcgatat cgagagtcag ccgttccttg aggcaatcag acagttccag catgaggtgg
      901 gacacgacaa tgccatcctg tgtcatgtga cactgattcc atatatcaag gcatcgggag
      961 agctcaagac aaagcctaca caggccagcg taaaggagct tcagggcatg ggaatccagc
     1021 cggatatcat tgtatgccgt tcagagcatg agcttgacca gaagcttaag gacaagattg
     1081 cactgttttg taacgtgcca aattcacacg tattacagaa ccttgatgtg gagtatctct
     1141 atgaggcacc acttgccatg gagcaggaga atcttgcaaa ggttgcatgc gagtgcttga
     1201 atctcgactg tcctgagccg gatcttgcag actggaagca gatggttgag gatctccgtc
     1261 atcctgataa agaggtaaag atcgcgcttg tcggaaaata tacagcactt cacgatgcat
     1321 acatttcagt tgttgaggca ctgaagcatg gcggtattcc tgagcatacc acagttgata
     1381 taaagtgggt tgactcagag gagcttaatg atgacaatgc agcagaggta ttcaagggta
     1441 ttcagggaat tatcgttccg ggaggctttg gagaccgtgg tgttgagggc atgattgcag
     1501 cagctaagta tgccagggaa aacaatatcc catatttggg actctgcctt ggaatgcagg
     1561 tggcaatcat cgagtatgcg cgccacgtat gtatgttcca tgatgcacac agtatagagc
     1621 ttgatccaaa cactactcat ccggttatcg cacttatgcc tgaccagaac ggaatagagg
     1681 atatcggagg tacacttaga cttggctctt atccatgtgt actcgacaag acatcaaagg
     1741 cataccaggt atatggcaca gagaatataa gcgagcgtca tcgtcaccgt tatgaggtca
     1801 acaatgacta cagaacagct ctcacagagc atggcatgaa gctttgcggt acatcacctg
     1861 atggcagaat cgtggagatg attgagattc cggagcatcc gtggtttatc gccacacagg
     1921 cacatcctga gcttaagtca cgtccgaatc gtccacatcc gcttttccat ggcttcatag
     1981 aggcggcaaa caaggtgaac agataatttt tcgttatatt taatacaata tttagaaagt
     2041 gcggttgatt ttgaccgcac tttttcttat aataaagtga agcttcgcac tttatatatt
     2101 gtgaggagac ggtttcatag gaatgactat aaaaattgca ggtcaaattt gctttgaagc
     2161 ttgtaataaa taagaaaaga acggagaaaa tatggataat cagggaccta ataactataa
     2221 ttataacaat ggcccgggca ataatgggtc cggtggaaac ggaaataatg gtggcaacag
     2281 accgggtgga aacggtggca gaaacaaccg tggcggacag ggtatcatgg catttatcct
     2341 gctgacactc gtggcattgt ttgtgtatgc acttatatca aacagcatat cgcatgccag
     2401 cacacaggaa aagtcataca gtgatttcat aaaacagctc gacaagggta atgtcaagag
     2461 tgttgagttt gattcctacg agatagacta caagcttgtg gatgacggac acaaggatta
     2521 tgacatcaca tattacacag gccgtgtggc tgatgatgag cttgtgccga cgctcaaaaa
     2581 agcaaagacc tcagagggca agtctattga gataaaggca gcgatacctg ataatacatc
     2641 tacatggatt tttaatatat taagctttat agttccgctt atactgctgt gggtgctttt
     2701 agcatttgtt tcaaagaaga tgggcggcag catggggatg ggagtcggca aatcgactgc
     2761 caaggtatat gtggagaagt ccacaggcgt caactttaag gatgttgcgg gacaggatga
     2821 agccaaggaa tctttacagg aggttgttga tttccttcac aatcctaaga gatatacaga
     2881 tatcggagcc aagcttccta agggagcact tctcgtggga cctccgggaa caggtaagac
     2941 actccttgcc aaggcagtag caggagaggc aggagtacca tttttctcac ttgcaggttc
     3001 agactttgtg gagatgtttg tcggagtcgg agcttcgaga gtgcgtgatt tgttcaagga
     3061 agcacaaaag atggcaccat gtattatctt tatcgatgag atagatgcaa taggaaagag
     3121 ccgtgacagc agatacggcg gtggcaatga cgagcgtgag cagacactaa accagcttct
     3181 tgcagagatg gatggctttg atacatcaaa aggattactt atattagcag caactaaccg
     3241 ccctgaggtg cttgacaagg cacttcttcg tccgggacgt tttgacagaa gaatcattgt
     3301 tgacaagcct gacttaaagg gacgtctgga gaccttaaag gtacactcaa aggatgtcaa
     3361 gatggatgag tcggtagacc ttgatgcact ggcacttgcc acagcaggac ttgtgggctc
     3421 agagcttgcc aacatgatta atgaggctgc catcaatgca gtcaaaaacg gcagacagct
     3481 tgtaaaccag tcagatcttt ttgaggcctt cgagcttgta gcagtcggcg gcaaggagaa
     3541 gaaggatcgt gtcatgagtg ataaagagag aaaaatcgtt tcataccatg aggttggaca
     3601 cgcnnnnnnn nnnnncttgt atcagccctg cagaaaaaca cagagcctgt gcagaaaatc
     3661 acaatcgtac cacgtaccat gggagcactc ggatatacac tccagacacc tgaagaggag
     3721 aaataccttg agacaaagga cgagcttctc gccaaaatca caacatatat ggcaggacgt
     3781 gcagcagagg tgctggtatt taactcagtc acaagcggag ccgcaaatga tatagaaaat
     3841 gccacaaaga tagcccgtgc catggtaact atgtacggaa tgtcagataa attcggcatg
     3901 atgtgccttg caacagtaca aaaccagtac ttagagggcg gagcaggnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tacgcagagg ccatgaagct cctcgatgaa
     4021 aacagagaaa tcctcgacag tatatcagat tatctctacg agaaggaaac aatcacaggt
     4081 aaggaattca tgaagatgtt ccgcgatatg aaaggactcc ctgacccgga cgaggagaac
     4141 aaagagcagg aggccacaca gaatgatgcg cagaaagatg tgacatcggc aacagatccg
     4201 ctcctccgta acgccataga caagcctgca gatacaaatg aatcttccgg atatacggcg
     4261 ccggatgata catcaaataa ttagtacctt atagggcaat tttcgaacac aacgagcaac
     4321 caagcaattt tcacgcagcg aaagagcgta taaaaaattc gtacaaataa agagcgcagt
     4381 agaaaagagt ttcgcacaca taaactgaaa aaagccggag ggtacatagt gtgttcagaa
     4441 ggaaaatatg aactgccgcg ggcttccaga caagagaagg gacgaaaaag ccttgcaaac
     4501 aaagaaaacc aaacggagag aaaccccgcg gctgacttca tattttcctg aggaatacac
     4561 tgtgtgccat gccggcaacc tcacaatctg aaagtgcgaa acttcaccac aacctaaaat
     4621 agcgaatatg gaaaaaacat ttgatatcaa agaagaatta aaaaaactcc cggatgcccc
     4681 gggagtttat attatgcatg acaaggatga tgtaatcata tatatcggca aagccaaaag
     4741 cctgacgaag cgagtacatc agtactttca ggcgagccac aatgagggca tcaagaaaaa
     4801 acagatggta gagcatatag atcattttga gtatatagtc accgactctg agcttgaggc
     4861 ccttgttttg gagtgcaacc tgataaagga gcatacacca aagtacaata ctatgcttcg
     4921 tgatgacaag acatatccgt acatcaaggt gacactggga gaggactatc cgagggtgct
     4981 gttttcgcgt cagatgaaaa aggacaaatc ccgttacttt ggtccgtata cgagtgcctc
     5041 tgcggtgaaa agctcgatag atctgataaa caaaatctat aagcttcgca cctgcaaccg
     5101 caggctgccg cgtgatatag gagcggaccg gccgtgcctc aactatcata tacaccagtg
     5161 cagtgcacca tgccaggggt atgtgacgaa ggaagagtat gccatatctg taaagggcgc
     5221 gatagatttc ttaaacggcg actatgagca gacattaaag gctcttagcg ataaaatgct
     5281 aaaggcctca gagagcatgg agtttgagaa ggcggctgaa tacagggatc tgataaacag
     5341 tgtgaa
//
LOCUS       DPUF01000268            3046 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_19440, whole
            genome shotgun sequence.
ACCESSION   DPUF01000268 DPUF01000000
VERSION     DPUF01000268.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_19440"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="DHV81_09230"
     CDS             <1..981
                     /locus_tag="DHV81_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCI94608.1"
                     /translation="EYPQYSRPVEWHDKKVPEVLMSGNQKKIDAWRLEKSIERTKERR
                     PDLYAGFKRLDKCREFLMKNKLLHIDMIELINRGCAEILFEADGEYLLRDMVSNVCFH
                     TRPDEGESKLIDLAPEDDTKPVDKYSSQHIPKTVTDQITNGIVLHQQRYVELFTANGF
                     NETVECMQAVYTNKEKLSVSGLYRPDGRPMPNGLIIRKLDAADIQEAAPMYPGFDNPD
                     YIIERIEAGAVYGAFFGDNTANDTINTLAGIIGIHEEGSIGMLYVKPQYRHQKLATAL
                     ETYAFNRALENGWIPYGQIIVGNDASMRLQESMGLHFSKSPVYWMTKNNA"
     gene            1123..1476
                     /locus_tag="DHV81_09235"
     CDS             1123..1476
                     /locus_tag="DHV81_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCI94609.1"
                     /translation="MNASEIIKSIENEQLKAEAPEFSVGDTVKVYGKIKEGNRERIQV
                     FEGTVIKKQNGSNRTTFTVRKLSNGVGVEKTWPLHSPNVEKVEIVRKGKVRRAKLYYL
                     RDRIGKAAKVKELVK"
     gene            1622..2206
                     /gene="lepB"
                     /locus_tag="DHV81_09240"
     CDS             1622..2206
                     /gene="lepB"
                     /locus_tag="DHV81_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCI94610.1"
                     /translation="MSRRRAGLSFRRQRRKFNLPLFKEIVSWVVELIVVIGLAYVLVS
                     FFGIRTNVVGQAMEQTLENDDNILVNRFAYIMSKPKAGDVIVFLPNGNNKSHYYVRRV
                     VAVPGDTVQIKDGALYVNDELYKESTDVASMEDAGLASDPIELEKDEYFVLGDNRNNS
                     EDSRYANIGNVKRDYIIGKAWFRFSGIGSFGTIK"
     gene            2246..3046
                     /gene="ylqF"
                     /locus_tag="DHV81_09245"
                     /pseudo
     CDS             2246..3046
                     /gene="ylqF"
                     /locus_tag="DHV81_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742365.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
     assembly_gap    2353..2445
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          977 a          496 c          766 g          714 t
ORIGIN      
        1 gaataccctc agtattcacg tcctgttgag tggcatgaca aaaaggtgcc tgaggtactc
       61 atgtcaggca atcagaaaaa gatagatgca tggcgccttg aaaagtctat tgagcgcaca
      121 aaggagcgca gaccggattt gtatgccggg tttaaaagac ttgataaatg cagagagttt
      181 cttatgaaaa ataaactgct tcatattgat atgatagagc ttataaacag gggctgcgcc
      241 gagatactgt ttgaggcaga tggagaatat ctgcttcggg acatggtctc aaatgtatgc
      301 tttcatacaa gaccggatga gggcgagtcg aagcttattg atttggcacc ggaagatgac
      361 acaaagcctg ttgataaata cagcagtcag catattccaa aaaccgttac agatcagatc
      421 acaaacggca tagtgcttca ccagcaaaga tatgttgagc tctttacagc aaatggattt
      481 aatgagactg tagagtgcat gcaggctgta tatacaaaca aagaaaagct atctgtcagc
      541 ggactgtaca gaccggatgg aaggccgatg ccgaatggct taattataag aaagctggat
      601 gcagctgata tacaggaggc tgcgccaatg tacccgggct ttgacaatcc ggactatatc
      661 atagaaagaa tagaggcagg ggcagtctat ggcgcttttt ttggtgataa tactgctaat
      721 gataccatta ataccctcgc tggaattatc ggaatccatg aggagggcag cataggcatg
      781 ctctatgtaa agccgcagta caggcatcaa aagcttgcca cagcacttga aacatatgca
      841 ttcaacaggg cactggagaa cggatggata ccatatggac agataatcgt gggcaatgat
      901 gcgtctatga ggctgcagga gagtatggga ttgcattttt caaaatcacc cgtatactgg
      961 atgactaaaa ataatgcatg aggtaggcaa aagatagtaa aaaatacttg caaatcccat
     1021 gaatctgtgg taaagtacca atgttgtgaa taaaagagat ggtcctctgt tgtgaaaata
     1081 gtagtagcat taagaacgtc aaaataatta ggaggtcaca aaatgaacgc aagtgaaatt
     1141 attaagagca tcgagaacga gcagttaaag gccgaggcac ctgagttttc tgtaggagat
     1201 actgtaaagg tatacggaaa aatcaaagag ggtaaccgtg agcgtatcca ggtattcgag
     1261 ggaacagtaa tcaaaaagca gaacggatca aaccgtacaa cattcacagt tagaaagctt
     1321 tctaacggtg taggtgttga gaagacatgg ccactccact caccaaacgt agagaaggtt
     1381 gagattgtac gtaaaggtaa agtacgtcgt gctaagcttt actacttaag agaccgtatc
     1441 ggaaaagctg ctaaggttaa agagcttgta aaataatatt tttacgcgaa agagaccatt
     1501 ttctgcgagc aatcgtatga gaatggtctt tttagttttc agggttacag cttgcaacaa
     1561 acatgaaaac aatatataat taagattacg caataaaata ttagttagca agaggtgtaa
     1621 gatgtccaga agacgagcag gacttagctt tagaaggcaa agaagaaaat ttaatctgcc
     1681 attgttcaag gaaattgttt cgtgggtagt ggagctgatt gtagtgatag gtctggcata
     1741 tgtgctggtg tcatttttcg gtatacgtac aaatgtggtg ggccaggcta tggagcagac
     1801 attggagaac gatgataata ttcttgtgaa caggtttgca tatatcatgt caaagccaaa
     1861 ggcaggagat gtgatagtat ttctgccaaa cggaaataat aagtcgcatt attatgtcag
     1921 gagagtggta gcggtgccgg gagatactgt tcagataaaa gacggcgcac tgtacgtgaa
     1981 tgatgagctg tacaaggaat caacggacgt tgcctcaatg gaggatgcag ggcttgcctc
     2041 agatcctata gagctagaaa aggacgagta ttttgtactt ggagacaatc gaaacaacag
     2101 tgaggacagc cgctatgcca atattggcaa tgtaaagaga gactatatta tcggcaaggc
     2161 atggtttaga ttttcaggca ttggaagctt tggaacaatc aagtagcaat agttataaca
     2221 tatatagata aaggacaggt gaattatgaa ttttcagtgg taccctgggc atatgaccaa
     2281 ggcaaagcgt cagatgcagg aggacattaa attaatagat ctggttatag agcttgtgga
     2341 tgcaagaatt ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccag ggagtggagt
     2461 gcatttttca aggaaaaggg cttttttgta gtcagtcttg atgctagaag caagtcaggc
     2521 atgaagagca tcacagatat cgtgatggag gcctgcaagg aaaagattga aagagaccga
     2581 aagcgtggta tcaaaaacag accggtaaga gctatggtgg tgggtattcc aaacgtcgga
     2641 aagtccacat ttatcaactc atacgccgga aaagcctgtg ccaagacagg aaacaagccg
     2701 ggcgtgacaa agggcaagca gtggatcagg ctcaataaaa gtgttgagct gctcgatacc
     2761 ccgggcatac tgtggccgaa atttgaggat cagacagtgg gacttagact tgcgctcatc
     2821 ggttcgataa aggatgagat tttaaatgtt gatgagcttg ccgtggagct tgtaaagttt
     2881 ctaaaagcag agtatccggg gcttttggca caaagatacg aggtaagcga ggaaaatgat
     2941 atcatagagc ttataagtgc tgtcgctgtc aacagaaaat gcctttcaaa gggaggagag
     3001 cctgattaca gcaaggcggc tgcactcatc atagatgatt tcagaa
//
LOCUS       DPUF01000269            3251 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_166, whole
            genome shotgun sequence.
ACCESSION   DPUF01000269 DPUF01000000
VERSION     DPUF01000269.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_166"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(2..388)
                     /locus_tag="DHV81_09250"
     CDS             complement(2..388)
                     /locus_tag="DHV81_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCI94611.1"
                     /translation="MLQAGIKGKKEITVTLDKTAKAMGSGTMDVFATPAMIALMENTA
                     FESVASELEEGSGTVGTALDIKHVSATPVGMKVTCESELVKVDGRALTFSVKAFDEAG
                     LIGEGTHERFIVFEEKFQKKADDKAK"
     gene            1144..3249
                     /locus_tag="DHV81_09255"
     CDS             1144..3249
                     /locus_tag="DHV81_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCI94612.1"
                     /translation="MTEKHGNLSIDSDNLFPIIKKWLYSDHDIFYRELISNGCDAITK
                     LKKLDMMGEYSLPADYKGKIQVLVNPDEKTIKIIDNGLGMTADEVEEYISRIAFSGAT
                     DFIEKYKDKSNDDQIIGHFGLGFYSAFMVADEVTIDTLSYKEGSTPVHWSCDGGTEYN
                     MEDGDMDDVGTEITLYLNDDCLEFANEYRAREVIEKYCSFMPTEIYLAKENAPEEYET
                     IDKADKRDTDTVIEEIHEDAKYEEKENDKGEKEQVEVSPAKDQLKIVKRPVPLNDTTP
                     LWTKSPNECTDEEYKEFYRKVFLDYKEPLFWIHLNMDYPFNLKGILYFPKINTEYDSI
                     EGTIKLYNNQVFIADNIKEVIPEFLMLLKGVIDCPDLPLNVSRSALQNDGFVKKISEY
                     ITKKVADKLIGMCKTDKEAYEKYWDDISPFIKFGCLKDEKFCDKINDYILFKDINDKY
                     QTLPELLAPVADDGKTDAESADASDDTKKAENTDANADSSANTDENKEPEKNTVYYVT
                     DVVQQGQYIKLFKEQGMNAVILDHNIDTSFITQLEQRNDHYKFMRIDADLTESLKDES
                     GADLTEATTTLSEVFKKALNNDKLTVKVENLKNADVASVLTLSEQGRRMQDMMKMYAA
                     GGMGGMDPSMFAADQTLTLNANNELVKYILDNKDSEHVPMFAEQLYDLAMLSNQPLSV
                     DQMTKFVKRSNDIMLLLTK"
BASE COUNT         1046 a          732 c          638 g          835 t
ORIGIN      
        1 tctactttgc tttgtcatct gccttcttct ggaatttctc ctcaaatact ataaaacgct
       61 catgagtacc ctcaccaatg agtcctgcct catcaaaagc ctttacagaa aaagtgagcg
      121 ctctaccatc caccttcaca agctccgact cacaagtcac cttcatgcca accggtgtag
      181 ccgaaacatg cttaatatca agcgcagtac cgacagtacc acttccctcc tcaagctcag
      241 acgcaacact ctcaaaagct gtattctcca tcagcgcaat catagccgga gtagcaaaca
      301 catccatcgt gccgcttccc atagccttcg ctgtcttatc caaagtcact gtaatctctt
      361 tcttaccttt aatacctgct tgtaacatat cagtctcctt atatctatat tttataaaat
      421 aaatcctagc atttttacgc cacaatgtcc agtgaaaaat aagtttataa acttttcaca
      481 attccaaaaa tgcccaatcc atgcggtttt ctgtcatttt gttagcactc gctatttttg
      541 agtgctaatt ttctattgac ttttccgaat tacagactta ttatcatatt gtgtttattt
      601 aacagataaa cctttaaatg ctatcaaatg attaaataag tctaactgat ttgatcatct
      661 tttaatgacc tacattcgcg gaaataagct aacattgcag gtagcagaca gttgttctga
      721 agagaaaata tgaactgcca cagggttcca ttacctgata aaatggcaca aacaaaagca
      781 tgccacaaac tatccataca aaatcctgtg gctgacttca tattttctct gaggaattat
      841 tgtctgctac gacgcaacct cacagcccat aaccgcggtg taaaacattt aactaaatct
      901 acactcgcgg aaataagcta aaattgcggg tagcagacag ttgttccgaa gataaaatat
      961 gaactgccac agggttccat taccgaacaa tgcggcacaa caaaaagcat gccacaaact
     1021 atccatacaa aatcctgtgg ctgacttcat attttctctg aggaattact ggctactaca
     1081 ccgcaacctc acagcctata atcgcggcgt aaatgtaaaa taaaaaacaa ggagtgtgta
     1141 aaaatgacag aaaaacacgg caatctttca attgacagcg acaacctgtt ccctattata
     1201 aagaaatggt tatattcaga ccacgatatc ttctatcgtg agctcatctc aaacggatgc
     1261 gatgccatca ctaagctcaa aaagctcgac atgatgggtg aatattccct tcctgcagac
     1321 tacaagggca agattcaggt gcttgtaaac cctgacgaaa agacaataaa gattatagat
     1381 aacggtcttg gtatgacagc tgatgaggtt gaggaataca taagccgtat cgctttttca
     1441 ggtgctacag acttcatcga gaaatacaag gacaagtcaa atgatgacca gattatcggt
     1501 catttcggtc tcggcttcta ctcagcattc atggtagccg acgaggtaac aatcgatact
     1561 ctctcttata aagagggctc tacacctgta cactggtcat gtgacggtgg cacagagtac
     1621 aacatggagg atggcgacat ggacgacgtc ggaacagaga ttactctcta cttaaacgac
     1681 gactgtcttg agttcgcaaa cgagtaccgc gcacgtgagg ttatcgaaaa gtactgttct
     1741 ttcatgccta cagagattta ccttgcaaag gaaaatgctc ctgaggagta cgagacaata
     1801 gacaaggccg acaagcgtga cactgacact gtcatcgagg aaatccatga ggacgccaag
     1861 tacgaggaaa aagaaaatga caagggcgag aaggagcagg tagaggtatc tcctgcaaag
     1921 gatcagctca aaatcgttaa gcgccctgtt ccgcttaatg atacaacccc actttggacc
     1981 aagagcccga atgagtgtac tgacgaggaa tacaaggagt tttacagaaa ggtattctta
     2041 gactacaagg agcctctttt ctggattcac ctgaatatgg actatccttt caacttaaag
     2101 ggtatcctct acttccctaa gatcaacaca gagtacgact caattgaggg aactattaag
     2161 ctttacaaca accaggtatt tatcgcagac aatatcaaag aggtcattcc tgagtttttg
     2221 atgctcctta agggtgttat cgattgtcct gatttaccgc ttaacgtatc acgttctgct
     2281 ctccagaatg atggcttcgt aaagaagatt tctgagtaca tcaccaagaa agtagctgac
     2341 aagctcatcg gtatgtgcaa gacagataag gaagcctatg agaaatactg ggatgacatc
     2401 agcccattca tcaagtttgg ctgcttaaag gacgagaagt tctgcgacaa gataaacgac
     2461 tatattcttt tcaaggatat caatgacaag tatcagactc ttccggagct gctcgcacct
     2521 gttgcggatg atggaaaaac agatgcagaa agtgctgatg cttctgatga tacaaaaaaa
     2581 gctgaaaata ctgatgcaaa tgcagacagc tcagccaaca ctgatgaaaa caaggaacct
     2641 gagaaaaata cagtttacta tgtcacagat gtagtacagc agggtcagta tatcaagctg
     2701 ttcaaggagc agggcatgaa tgctgttatt cttgaccaca atatcgatac ctcattcatc
     2761 acacagcttg agcagagaaa tgaccactac aagttcatga gaatagatgc agacctcaca
     2821 gagtctctca aggatgaatc cggtgcagac ctcactgagg caaccactac tctctcagag
     2881 gtattcaaaa aggctttgaa caatgacaag ctcactgtaa aggtagaaaa ccttaagaac
     2941 gcagatgtag catctgtgct cacactctca gagcagggca gacgtatgca ggatatgatg
     3001 aagatgtacg cagccggagg catgggtgga atggatccaa gcatgtttgc tgctgaccag
     3061 acactcacat taaatgccaa caacgagctt gtgaagtaca tcttagacaa taaggattct
     3121 gagcacgttc caatgtttgc tgagcagctt tacgaccttg caatgctttc taaccagccg
     3181 ctttcagttg accagatgac taaatttgta aagagaagta atgatatcat gcttcttctc
     3241 acaaagtaat c
//
LOCUS       DPUF01000270            2982 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_14221, whole
            genome shotgun sequence.
ACCESSION   DPUF01000270 DPUF01000000
VERSION     DPUF01000270.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_14221"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="DHV81_09260"
     CDS             <1..555
                     /locus_tag="DHV81_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94613.1"
                     /translation="LERLAYAIIIVMVATMFAWIMALLLRLQPMDFEDLNLTPVLHLI
                     LRLIMSFFGVFGFSIMFNSPAPMAATAALIGAIANSLRLELVDLTGMPAPAAAFAGAL
                     TAGLLASFIKENNGYPRISLTVPSIVIMVPGLYLYRAIYNFGIMALSDAVSWFASAIM
                     IIIALPLGLIFARILTDKTFRYCT"
     gene            668..1969
                     /locus_tag="DHV81_09265"
     CDS             668..1969
                     /locus_tag="DHV81_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCI94614.1"
                     /translation="MLTAVTGINWGDEGKGRVIDLLAEHADIVVRYQGGNNAGHTVVN
                     KQGKFVLNLLPSGILHPDVVCVLGDGMVIDLNHLSNEIAQIRTQDVSVSPKNLKLSTR
                     ATISMPWHKIQDELEEERLSKSGAAFGSTKRGIAYAYSDKYRKKTIRLGDLLHLDEEN
                     VQARLKTMLEAKNLELAGCYHQEPMSLSALLSWCRQKAAKYAPYITDTGAFLEDALSK
                     GKRVVLEAQLGAMRDIDYGIFPYTSSSSTISAYGPIGAGIPGASLDHVVGVLKAYSTC
                     VGAGPFVAEKAMNGEWTEMVRKYGGEYGAATGRPRRVGPFDAVASRYGLKCQHADKIA
                     LTKLDVLSIMDQIPVITGYQYKNAVTNVFDPTENLEKYTPVVKMLPGWKTDISNCRSY
                     DELPVNAKRYINFLEDMLRHEIQFVSVGAERNQYLLNGKWL"
     gene            complement(2013..2870)
                     /locus_tag="DHV81_09270"
     CDS             complement(2013..2870)
                     /locus_tag="DHV81_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCI94615.1"
                     /translation="MYDSIIIGTGPAGLSAALNLKTYKKDFIWFGSKNLSEKVQKAEK
                     ITNYPGFSQISGQELFERFDAHRRAAGIDITEKTVTNIMPAGNGFMVLADNEMYEART
                     LILCMGVMSAKQLDREDELLGKRLSYCATCDGMFYKDKRIAVICNDKKYEHEVKYLAD
                     LAAEVLYFPTYNDSEIELPNVKISKDAPIALIGDSFVEGITLRSGKTESVDGVFCLRN
                     AIAPSKLMPQLEIENGHILVDRAQQTNVAGCFAAGDCTGRPYQYTKAVGEGNVAAHSC
                     IQYLSKSEK"
BASE COUNT          808 a          704 c          619 g          851 t
ORIGIN      
        1 cttgaacgcc ttgcctatgc aataataata gtcatggtag ccacgatgtt tgcatggatt
       61 atggcactgc ttctgcgact gcagcctatg gactttgaag acttgaatct gacccccgta
      121 ctacatctaa tcctgaggct tataatgagc ttcttcggtg tgtttggatt ctcaatcatg
      181 ttcaacagtc cagctcccat ggctgcaaca gccgcactta tcggtgccat agcaaattca
      241 ctgcggcttg agctggtaga cctgacaggt atgccggctc ctgctgccgc atttgccgga
      301 gctctcacag cagggcttct cgcatcattt atcaaggaaa acaacggata tccgagaata
      361 tcccttactg ttccatccat agtcataatg gttccgggac tttatctgta tcgggctata
      421 tacaatttcg gcataatggc actttcggat gctgtttcat ggtttgcatc agcaatcatg
      481 attatcattg cactgccgct cggactcatc tttgcaagaa tactaactga taagacgttt
      541 aggtattgca cgtaacagaa tcatacacat tataagcaga cttgttttat accattcaaa
      601 atccggacag tacaagctct gcaccaacat acaaatatta aactcaaaaa acaaggaggc
      661 attcatcatg cttacagcag ttacaggaat aaactgggga gacgagggaa agggtcgtgt
      721 catcgacctt ctcgcagaac atgcggatat cgtagtccgc tatcagggtg gtaacaatgc
      781 cggacacaca gtagttaata aacagggaaa atttgtctta aatctgcttc catccggaat
      841 acttcaccca gatgtagtat gtgtgctcgg tgacggaatg gttattgacc taaaccatct
      901 gtccaatgaa attgcacaga tcagaacaca agatgtatct gtttcaccaa aaaacctgaa
      961 attatcaaca agagccacaa tttcaatgcc gtggcataaa atacaggatg agcttgagga
     1021 agagcgcctt tcaaagtccg gagcagcctt cggctctacc aaacgcggta ttgcctatgc
     1081 atacagcgat aaataccgta aaaaaacaat ccgtctcggt gatttactcc acttagacga
     1141 agagaatgta caggccagac ttaaaaccat gctagaagct aaaaaccttg agcttgcagg
     1201 ctgctatcat caggagccta tgtctctgtc agctcttttg tcatggtgca ggcagaaggc
     1261 tgcaaaatat gcaccttata tcacagatac cggtgcattc cttgaggatg cactatcaaa
     1321 gggaaagcgt gttgtacttg aagcacagct tggtgcaatg agggatatag actatggtat
     1381 tttcccatat acttcaagct cctccaccat ctcagcttac ggtcctatag gtgccggtat
     1441 ccccggtgcc tcattagatc atgtggtcgg tgtactaaag gcatactcta cctgtgtcgg
     1501 agccggacca tttgtcgcag aaaaagctat gaacggtgaa tggactgaaa tggtccgcaa
     1561 gtatgggggc gaatatggtg ctgccactgg aagaccccgt cgtgtgggtc cgtttgatgc
     1621 cgttgcaagc cgctatggtc taaaatgcca gcatgctgac aaaatcgcac tgaccaagct
     1681 ggatgtcctg agcatcatgg atcagattcc tgttatcaca ggatatcagt ataaaaatgc
     1741 tgtaacaaac gtctttgacc ctaccgaaaa tctcgaaaaa tataccccgg tagtcaaaat
     1801 gctgccgggg tggaaaaccg atatatcaaa ttgcaggtca tatgatgagc tgccagtaaa
     1861 tgctaagcgc tacataaact tccttgagga catgctaaga cacgaaatac aatttgtctc
     1921 agtcggagct gagagaaatc aatatcttct caatggtaag tggctgtaat tgcctttaac
     1981 atgctttaac cgacctctat gccatcgcca tattattttt ctgatttact aagatattgt
     2041 atacacgaat gtgctgccac atttccttct ccgacagcct tcgtgtactg gtatggcctg
     2101 cctgtgcagt ctcctgctgc aaaacagcct gccacatttg tctgttgggc tctatccacc
     2161 aatatatgtc cgttttctat ttcaagctgg ggcataagct ttgatggtgc gatggcgttc
     2221 ctgaggcaga atactccgtc tacgctctca gtctttccgc ttcgaagcgt aattccttcc
     2281 acaaatgagt ctccgattaa tgctataggt gcatcctttg atatctttac atttggaagc
     2341 tctatttcgc tgtcattata ggttgggaaa tacaatacct ctgctgcaag atccgcaaga
     2401 tattttacct catgctcata ctttttgtca ttgcatatga cagctatcct tttatccttg
     2461 taaaacatac catcacaggt ggcacagtag ctgagcctct ttccaagcag ctcatcctcc
     2521 ctgtccagct gctttgcaga cattacaccc atgcaaagta tgagagtcct tgcctcatac
     2581 atctcattgt ctgcaagcac cataaagccg tttcctgcag gcatgatatt ggtcacagtc
     2641 ttttctgtta tatcaattcc cgcagctctc ctatgagcgt caaagcgttc aaacagctcc
     2701 tgtccgctta tctgtgaaaa tccgggatag tttgtgattt tctctgcctt ttgcaccttt
     2761 tcgcttaggt tcttcgaacc aaaccagata aaatcctttt tatatgtttt taaattcagg
     2821 gctgccgaca gtccggcagg acctgttcct attattatac tatcgtacat gctatgctcc
     2881 ttgtaccaaa tatttgtata tatttagacc ttatatatag tgtggggcac tatggtatac
     2941 atcgaattgc atgcaagtat gcatcgatga tatcttttga cc
//
LOCUS       DPUF01000271            3360 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_49460, whole
            genome shotgun sequence.
ACCESSION   DPUF01000271 DPUF01000000
VERSION     DPUF01000271.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_49460"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(1..2100)
                     /locus_tag="DHV81_09275"
                     /pseudo
     CDS             complement(1..2100)
                     /locus_tag="DHV81_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515905.1"
                     /note="frameshifted; too many ambiguous residues;
                     incomplete; missing stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     assembly_gap    459..556
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2355..2900)
                     /locus_tag="DHV81_09280"
     CDS             complement(2355..2900)
                     /locus_tag="DHV81_09280"
                     /inference="COORDINATES: protein motif:HMM:PF15714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCI94616.1"
                     /translation="MKITGLTRRVDSLGRIVIPKELRRMLHIKEGSPLEIYMDADETI
                     VLKKYSQVGSLKNISQAYAQSLSKVTGAMVCVADRDEIIAAAGKNHKNYIGKALSKEL
                     ENIMDKRKSALYSKKDDTLIPVVKDDKADCEAQAIAVIVHDGDSAGAVILCVYDEKAD
                     KSEIAEKLAGAAAEFLADQLG"
     gene            complement(3019..>3360)
                     /locus_tag="DHV81_09285"
     CDS             complement(3019..>3360)
                     /locus_tag="DHV81_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HCI94617.1"
                     /translation="AVQEQKKTGMQDYYMFAVADQPYLKSQSVIKLIDKVLENKGNMK
                     SVFSLRCGDTVGNPCVFHSSLIPQLLSLEGDKGGRSVAKKYDCVYVDIADERELTDID
                     TLSNSKHTVTL"
BASE COUNT          790 a          772 c          606 g         1094 t
ORIGIN      
        1 gttcctgcat caggtgtggt cacagtctga tctgtaggat tattctgcgt ctggtcatcg
       61 gcaggtgtat cctggctgtc ctgataatca tccgatattt gggcatatgg caggaaatct
      121 attggtgtaa ggtcagcatg tatctgatcc atgtagttct tccatatttc tgccgggtaa
      181 gagcttcctg tcagtttttt gactgtctca ggcatatcgc agcctaccca tacacatgtg
      241 gtgtagtatc tggtaaagcc acagaaccag ccgtccttct tatcgtttgt ggttccggtt
      301 ttgccggcgc aaggcatgcc attgtccaga ttagcgctac gtccggttcc tgatttcata
      361 acatctgtca tgacatctgt catcatacgc gatgctgtct ctgtatatat gacggtatcc
      421 ttctgcgatg atgtatatat gatattgttg tcagaatcnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnngttg taaaacctcc aagtgctgtt gctgtgacat agtcatcctt
      601 tacaatattg gtaaaattca tatccttgag atactgtaag cctacctttg gcgtgagctc
      661 atcatatagc tgccatgcta cagtattcag tgacttttga acggcaaacc tgatagtcac
      721 atcaccgtaa tatgaatcac ccgagttaga cggtcctcca tcaaacttgt ggtcatttac
      781 gatggtatcc ggtgtcttac cacgctcaaa tgatggtgta tacacaatga gcggcttgat
      841 tgagcttccc ggctgacgat ggctttggaa agcacggttt agcgtatgac tgaccgcatc
      901 ctggcttcgt ccaccaacta ttgcaaccac ctcgcctgtg tcgttgtcta tacaggttgc
      961 agcactctgc agcttaaatg tgccatcatc ggtcgtgtct gtaaagtcca aaagcgtcag
     1021 gtcaattgaa tcctgaagtt gctgctgcat agtcagatca attgatgtat aaatcttgta
     1081 accgtctgag tagagctttt tctgacaata tgaatacatc tcatcatatg aagcatcata
     1141 ctccttttct tcatcgtctg agtcaaagta atacttgaac ttgaagccct cattctccat
     1201 gagcgccctc gtagcgcagt aatatgcata ggtatccaca taattgtact tgaccgtgtc
     1261 atccttctca ggcatgttga gggttatctc ctcgtttata gcggcctcat attcctgctg
     1321 tgtgattttg ccatcttccc ttagattttt caatataaga ttgcgacgtg tcaatgtatg
     1381 atccttgttg tttatcgggt catagtatgt cgggctgttc ggaatcgcac acaaaaaggc
     1441 agtctgcgac aaatcaagat ccgaaagctc acagttaaaa tagccatggc acgctgcctg
     1501 aataccataa tatccgttca tgaaatagac attattcagg tagaactcca taatatcgtt
     1561 cttggaatat ttcttttcaa gctccatcgc tatgaacatt tccttgactt ttcgctgcca
     1621 gttcttgttc gtatccagat aaatatttcg ggcgagctgc atcgtgatgg tactgccacc
     1681 ctgggtaatt ctcttgtttt ttataattga ctttgcggca cgcactattg cctttaggtc
     1741 cacaccattg tgcttataga acttcttgtc ctcgatactg accatgcagt ttacgaagtt
     1801 ctgcggtata tcctcatact tcacatagtc agcctttttg gtagtggccg tctcggatat
     1861 ctcatcaccg tttgtatcgt agagcgtact cgtgcgcgcc ggtaaaaatg tgtttttgtc
     1921 agatttctgc acgagctcta ccgcctctga gtgaagcttc gccaccttgt ctgcatagcc
     1981 tccaaagtaa taaaagcata atccaccgag aatcagtacc atcagaaaaa tctgaagctt
     2041 tatgaaaaac cagaaaacgc gatgctgctt tttctttttt ttctttttct gttttgccat
     2101 ttctaaacgt tacctccaaa ctatcggtat accattgtat atcggcatac tttatagata
     2161 attgacaact atatccaata tgtactgtct attatactac aatgcctcta catcagctaa
     2221 ttaataattg cttaattttc tattatacct ttatagcaca aaaggaagct aattgctccc
     2281 tttatcaagc tattttacat ctttattact gttgtagtca agtaaatagt tttttacaat
     2341 gcttttcgct atctctagcc aagctggtca gccagaaatt ctgctgctgc acccgccagc
     2401 ttttcggcaa tctcagactt atcggctttt tcatcgtaaa cacacagtat aacggctccc
     2461 gcgctatcac catcatgcac tattactgct attgcctgcg cttcacagtc agctttatcg
     2521 tcctttacca caggtatcag ggtatcgtct tttttactgt acaatgcact ttttctctta
     2581 tccataatat tctcgagttc ttttgacaaa gccttgccta tataattttt gtggtttttg
     2641 ccggctgccg ctattatctc atccctatcc gccacacata ccatggctcc tgttaccttt
     2701 gacaggctct gggcatatgc ctgtgatata ttcttcaggc ttcccacctg cgaatacttc
     2761 tttagcacta tcgtctcgtc cgcatccata tatatttcca gtgggcttcc ctcttttata
     2821 tgcagcatcc tgcgaagctc ctttggtatg actattctgc cgagggaatc tactcttctg
     2881 gtcaatcctg tgattttcac tttattgtcc tccggttact tttttgcatt tgcattcatg
     2941 ctttggaggt agtatatggg aatttgaata aaatatacat tcgatatgca ctgatacagt
     3001 gctctttcat aatagctttt ataatgtcac ggtatgtttt gaatttgata aggtatctat
     3061 atccgtcagc tcacgctcat ctgcgatatc cacatataca cagtcatact tctttgccac
     3121 acttctgcca cccttatccc cctccagtga taaaagctgc gggataagcg atgaatgaaa
     3181 tacacacgga ttacctacgg tatctccaca gcgaagtgaa aaaactgatt tcatatttcc
     3241 tttattttca agcactttat ctattaattt aatcacgctt tgacttttta aatatggctg
     3301 gtcagctact gcaaacatgt agtagtcctg cataccagtc tttttctgct cctgcaccgc
//
LOCUS       DPUF01000272            3507 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_7852, whole
            genome shotgun sequence.
ACCESSION   DPUF01000272 DPUF01000000
VERSION     DPUF01000272.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_7852"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            108..758
                     /locus_tag="DHV81_09290"
     CDS             108..758
                     /locus_tag="DHV81_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCI94618.1"
                     /translation="MDKVFKIEDLRPIRDEMTISRNSGLSDKTPITYFSLGKGTSISQ
                     DSYDNTTVYIVAKGCADFLIGAYAAKHTISEGDMLVVPSKTLCGVTTETGCIYTEIII
                     KKENNNMNKIIKSGEVMKLKDLISYEDGSITNIDVVSNDTMKFVLMAFDEGTGLTPHR
                     APGNAIIFALEGKAVIDYEGKDYTISAGENFRFDKNGLHSVTADERFKMGLLLVLE"
     assembly_gap    760..823
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<824..2222)
                     /locus_tag="DHV81_09295"
     CDS             complement(<824..2222)
                     /locus_tag="DHV81_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI94619.1"
                     /translation="MKDLTKGKPSRLILAFAIPIFMANILQLTYSIVDTRIVGSYLGE
                     NSLAAVGATTTLSNLMIGFLMGLANGFAIITAQKFGARDYAGVKKSFALSIKMGCIIA
                     LAITVLCLLFLRQILGFLNVSNYLMGMAVSYIFIIIAGLVATFLYDACAAALRALGDT
                     VTPLVILAVSVCLNMAGDIFFVVVLKAGVAGAAIATVLAQVIAFIVCYVYMVKRYELL
                     RLSRSDLFDSHLLRRHLKSSDENCDTEGAECKDSENNMTPEVIAVMKMTMLKAGLSMA
                     FMSSLVNIGSLTLQTAINRLGQDFIVAHTAARKISEIFMVMFTVFGQTMATFCGQNIG
                     AGEVARVKKGIKLAIFYTCIWCTMAIIASYTIGPWLVKLVTGTNNPIVIKNATNYLKF
                     DTLFYYVTAVICIVRNAMQGLGEQITPLVSSSLEMVGKIVIAFTLVPLLGYTGVIVAE
                     PIVWFIMVIPLLVKIY"
     gene            2467..2658
                     /locus_tag="DHV81_09300"
     CDS             2467..2658
                     /locus_tag="DHV81_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCI94620.1"
                     /translation="MPIKPTEMERIILADGWIFKNQTGSHRHYIHPSKPGKVTIPFHK
                     GKELNKITENSIRKQAGIK"
     gene            2680..3108
                     /locus_tag="DHV81_09305"
     CDS             2680..3108
                     /locus_tag="DHV81_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HCI94621.1"
                     /translation="MLSIYPACFFKEDNGYSVIFPDLNYLATQGDTLEDAVAMAVDCL
                     AGYLYTAKMDNEKFPKASKLSDINIDRLSDELDITGTYTDAFTNMVSVDVKAYAKEHF
                     DKSVRKTLTIPAWLNTAAQEEGINFSKTLQEALMSKLKAH"
     assembly_gap    3187..3313
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3314..>3507
                     /locus_tag="DHV81_09310"
     CDS             <3314..>3507
                     /locus_tag="DHV81_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCI94622.1"
                     /translation="HQMGEGSTVYESLVKKFEYIRMEQLFFKAAFKNDEQNNLKEHDF
                     ELIRQFYIDQIEGKSHRKIS"
BASE COUNT         1080 a          682 c          612 g          942 t
ORIGIN      
        1 attatattgt ttttgttgta tatgcaacat atttttggac aaaattatag taatattgta
       61 ttataaataa tgaaatcaac attcaagaaa gaaggacaag aaatcctatg gacaaggttt
      121 tcaaaataga agatttacgc cctatccgcg acgagatgac catctcacga aattcagggc
      181 taagtgacaa aacacccata acatattttt ctctcggtaa gggcacgtca atctctcagg
      241 attcctacga taataccacg gtatacatcg ttgcaaaagg atgcgctgat tttcttatcg
      301 gagcttatgc tgcaaagcat accatatcag aaggtgatat gctggtagtc ccttcgaaaa
      361 cactctgcgg agtaacaaca gaaaccggct gtatttacac cgaaatcatc ataaaaaagg
      421 agaacaacaa tatgaacaaa attatcaaat caggcgaggt catgaagctt aaggatctta
      481 tctcatatga ggatggcagt atcacgaaca ttgatgtggt aagcaacgac acaatgaaat
      541 tcgtgcttat ggcattcgat gagggcaccg gtctcacacc gcatcgcgcg cctggaaatg
      601 caataatttt cgcactcgag ggcaaggctg taatagacta cgagggcaaa gattacacaa
      661 tctcagcagg cgaaaatttc cgttttgaca aaaacggact gcacagcgta accgctgacg
      721 aaaggttcaa gatgggactt cttctggtgt tggagtaaan nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgtatat cttcaccaaa
      841 agcggtatca ccatgataaa ccatactatc ggctctgcca ctatcactcc tgtatagcca
      901 agcagtggca caagtgtgaa tgcaatgaca atctttccca ccatctcaag tgagcttgat
      961 acaagcggtg ttatctgctc tccaagcccc tgcattgcat ttcgcactat acatatgaca
     1021 gcggtcacat aataaaacag agtatcaaat ttgagataat ttgtggcatt ttttatgaca
     1081 ataggattat tagtgcctgt tacaagcttc accagccacg ggcctatagt gtagcttgcg
     1141 ataattgcca tcgtacacca tatgcaggta tagaatattg ccagttttat gccctttttc
     1201 actctggcta cttctcccgc tcctatattc tgcccacaga aggtcgccat tgtctgtcca
     1261 aacacggtaa acatgaccat gaatatctcc gatatctttc tggcagccgt gtgagctact
     1321 ataaaatcct gtcccagtcg gtttatggcg gtctgcaggg tcagcgagcc tatatttaca
     1381 agtgaactca taaatgccat ggaaagaccg gccttaagca tcgtcatttt catcacggct
     1441 attacttcag gagtcatatt attctcgctg tctttacatt ctgctccctc ggtatcacaa
     1501 ttttcgtcgc tagatttcag gtgtcttcgc agtaaatgac tgtcaaataa atcactgcgg
     1561 gacaatctca aaagctcata tctttttacc atatatacat agcacacaat aaacgcaata
     1621 acctgtgcaa gtacagtggc aatcgcagcg cccgccactc ctgccttcag tacgaccaca
     1681 aagaaaatat cacctgccat attcagacag accgagacgg ctagtatgac aagcggcgta
     1741 actgtatcgc ccagtgccct aagtgccgcc gcacatgcat catacaaaaa tgtcgctaca
     1801 agccctgcta ttataataaa aatatatgac acagccattc ccatcaaata atttgataca
     1861 tttaaaaatc caagaatctg acgcaaaaat aacaggcaca ataccgttat agcaagcgct
     1921 attatgcatc ccattttaat tgacagtgca aacgatttct ttacaccggc atagtccctt
     1981 gcgccaaatt tctgggctgt tattatggca aatccattag ctaagcccat caaaaagcct
     2041 atcatcagat tgctgagtgt cgtggtggct ccgactgctg ccagtgagtt ttctcccaga
     2101 tatgatccga ctattctcgt gtcaactata ctgtatgtga gctggagtat atttgccata
     2161 aatattggaa tggcaaatgc cagtattagt cttgatggtt ttccttttgt aaggtctttc
     2221 atgtatgtat atcctcgtat atttcaaaat attttcatca atatattagc agataaattt
     2281 taaaattcaa tctttttaat cggtaatata atcatttctg atcatactaa aatataaaca
     2341 acaatatata gtttatatac gcaataatta tacatattaa atttatatat attatattta
     2401 cacgcattga atttatacgt atcatatgtt atattatata tatactaaag aaatgtggat
     2461 aaaaatatgc cgatcaaacc aactgaaatg gaacgtataa tacttgccga tgggtggatt
     2521 ttcaaaaacc aaaccggatc acatcggcat tatattcatc cttcaaagcc cggaaaagta
     2581 actattcctt ttcataaagg caaagaactg aacaaaatca ctgaaaattc tatccgtaag
     2641 caagcgggaa tcaaataatc ataaggaggt atactcatca tgttatcaat atatccggca
     2701 tgttttttta aggaagataa tggatactct gtaatctttc ctgatttaaa ttatcttgca
     2761 acacaaggag atacccttga agatgctgtg gccatggctg ttgattgtct tgcaggatat
     2821 ctgtatacgg ctaagatgga taatgaaaaa tttcctaaag catccaaatt atctgatatc
     2881 aatattgaca gactatctga cgaattagac ataacaggaa catataccga tgcatttacc
     2941 aatatggttt cagtagatgt taaggcatat gccaaagagc atttcgataa atcggtccga
     3001 aaaaccctta ccattccggc ttggctaaat actgctgcgc aggaagaggg aattaatttt
     3061 tcaaaaacac tgcaggaagc acttatgtca aagcttaagg ctcattagat atctgcatta
     3121 taattcggag acattatgaa caacgatata aaatacaagc tggccaatgc catgaaggaa
     3181 tgcatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnncaccaga tgggtgaggg cagtaccgtg tacgaaagcc ttgttaaaaa
     3361 atttgaatat atccggatgg agcaactttt ctttaaggct gcttttaaaa atgatgagca
     3421 gaacaatcta aaggagcatg attttgagct gattcgtcag ttttatattg accagattga
     3481 gggaaaaagt caccggaaaa tatccga
//
LOCUS       DPUF01000273            7427 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_12861, whole
            genome shotgun sequence.
ACCESSION   DPUF01000273 DPUF01000000
VERSION     DPUF01000273.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_12861"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="DHV81_09315"
     CDS             complement(<1..1864)
                     /locus_tag="DHV81_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94623.1"
                     /translation="MKKLFGCILSLVVAVMLVFTPAEHVYAQGLGLSVSSSSIAVGKT
                     VKVTVSMPSGYFGTVVISSSDEGVLSNGGDGVANIGDAAGYPTSQSFSFTAKAAGSCT
                     IKAYCTVVGDAEGNDAGGTITGASTKVTVTSASSNNDSNSNKDNKDNTGNDNNANKDN
                     ENKEEKKSSNASLGSLVISAGTLSPEFSAATKDYTATVDYSCSSLAVTANPADSKASV
                     TSVTGNDSLEVGENTVSVVVTAEDGSTSTYNIVVTRRAEDDPENADKQDNWKKFDING
                     TEWTMVNDIPEDVVPEGFEHSKTVIDGLEYNTLHGTFGDITLVYLQSESGNGLFVYDA
                     AQNAAYEFVRINSESHFIVVLLPKVDDVPEGYNEISLSIEGKGVATAYQTKAEKTDDQ
                     TKDFYLVYAINDNGESGWYTYDSVDGTYMRTELSTPTVAQEENDTTKSELVPGIANKY
                     LVLAAILVLIIVILLLLLIVSAVKNRKYKAMDYHDDDDDVDDAAEDVSNEALEETTDE
                     TADEIADELEEEVTEEPLDEVAEEAAEESVDEVAGEAAEESAGEAAEETADEAVEEPA
                     DEVAEETAEGAADEQKMATDENISNASYVGRTVEITPDSKKAVENENSEFDLKDD"
     gene            complement(1881..>2939)
                     /locus_tag="DHV81_09320"
     CDS             complement(1881..>2939)
                     /locus_tag="DHV81_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCI94624.1"
                     /translation="KNGLSFAKKQGWNTRVKSISGGAVKVGSNYINKGQNTLYFEKFN
                     VVNTSSLYFHQYMGNATAALTEGQSLAKGYSDKNQAFVFKIPVYNNMPSSAVGFNKAG
                     DTNNYLQSLAISGVTLTPAFNGTTTSYSAVVSNAISSVTVSADAVSGNSGVSGTGSYS
                     LAVGNNTIKVKCKSQSGDTRTYTININRQAASANNAGGNNNQNNNNQNNTDVNITSGK
                     YSIGTYITGIEPGTGAADFVKNIAVSASGTVKLLTSSGSENSGKIATGNKVAVYDASG
                     NLKKTYDIVIYGDINGDGAVNALDMIKLNRHILGKGTLTGAYLEAADANRKGDGGNAL
                     DMIIMNRHILGKSKISQN"
     assembly_gap    2940..3090
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3091..4247)
                     /locus_tag="DHV81_09325"
     CDS             complement(<3091..4247)
                     /locus_tag="DHV81_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCI94625.1"
                     /translation="MIKINKFLAVSVVTLSLAACDLAPYSSIAVYADQAAKVVCDERY
                     WVAARSGPGTEYELEHKLSNGKDITILEQTTGADGKVWYKAKYNLAANNEECVSYIRS
                     DFVSAGTGTAASDNAQPGDASADTGNADAIQQNNEEQLALATASGAYATGTITGGNVY
                     VRNAAGTSGTTKVVSLNWNHQVDIIGETRVNGVVWYNVKGTLNGKAFTGWTISTYIKV
                     TYNNSGDNTDFVTAMKNAGFPDSYIPNLTALHNKYPSWTFEAVNTGLNWGTVIENESV
                     NGLNLVSKSADNAKKSTAAGAYNWSTNTWVEYEPGWVSASSAYIAYLMDPRNFLDETN
                     IFQFQSLAYSPNEALEGVKSIVKGTFMEGTKTYSNNGEKINYASTFMDVAK"
     gene            complement(4458..>5255)
                     /locus_tag="DHV81_09330"
     CDS             complement(4458..>5255)
                     /locus_tag="DHV81_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HCI94626.1"
                     /translation="NDKPYLSLGADLTPDQRATVLGFMGIELTDLDKYDVVYVNNDEE
                     HKYLDSYISKSEIGTRSLSSVLITEDKKGAGLSVSTHNINYCTVGMYKNALATAGIAD
                     AKIIVAGPFPISGTAALVGTLKAYEEMTGKKLDDKVTDAAMDELVTTGELNKSIDGDS
                     QDIEAMIADLKKQLADGGLKDESQIKDAIKEAAKDYDLKLSDDDIAKLTSLLMKLKDA
                     NIDWDSVINQAQDWASKLGDKINDPGFWEKLGNFFMDLWDKIKSLFS"
     assembly_gap    5256..5403
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5487..6524)
                     /locus_tag="DHV81_09335"
     CDS             complement(5487..6524)
                     /locus_tag="DHV81_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCI94627.1"
                     /translation="MRYPEFLKDRGTIGFVAPSMGCTTEPYRSAFDNAIKRFHSMGYK
                     TIEGPNCRCDSGIGISNTPKKCAEELNESYASADSDVLISCGGGELMCEVVPYIDFER
                     VKQSKPKWYMGYSDNTNFTFLEATIADTAGIYGPCAGSFGMEVWHESLTDAFNLLCGR
                     KLSFTNYPMWEKESIKDENAPFVGYNLTEKSCISSIPAVAKGSRLTMRGRLLGGCMDC
                     LINLLGTSFDHVREFNERYSDDGIIWFLEACDLNVMGMRRAMWQMKNAGWFSNVKGFL
                     IGRPAHFGEEMFGIDHRQALSSLLREFNVPIIMDLDIGHMSPMMPVVCGSMADVSFDG
                     SNFTISYDIEQ"
     gene            complement(6521..6820)
                     /locus_tag="DHV81_09340"
     CDS             complement(6521..6820)
                     /locus_tag="DHV81_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94628.1"
                     /translation="MKNIEEQQIIIECPNTIFHLYIDSEDELLKVKAFMNNMKHVDSI
                     SLHDIYNWCNRQHVQYTTTFNYDSKMTLTEMIKSYIFYFRQKLRYVNNSDRMIET"
     gene            complement(7031..>7427)
                     /locus_tag="DHV81_09345"
     CDS             complement(7031..>7427)
                     /locus_tag="DHV81_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCI94629.1"
                     /translation="YSRAKDYPSVAKQALSEMVRQQPDVVIDDATGLIQKGVIVRQLL
                     LPGHVNDAKAVLKYLYDTYHDHVYISMMSQFTPIALKDYPEINRTVTRREYERLIDYA
                     LEIGITNAFIQEGDVAKDSFIPAFDCEGV"
BASE COUNT         1764 a         1819 c         1270 g         2275 t
ORIGIN      
        1 aatcgtcttt taaatcaaac tcagagtttt cattctcaac agctttctta gaatcaggtg
       61 taatttctac ggttcttccg acataggatg catttgatat attttcatcc gtagccatct
      121 tttgctcatc tgcagcacct tccgctgttt cttctgcgac ttcatctgcc ggctcttcta
      181 cagcttcatc tgctgtttct tctgcggctt cacctgccga ctcttctgca gcttctcctg
      241 ccacttcatc caccgactct tctgcagctt cttctgccac ttcatctaac ggctcttctg
      301 taacctcttc ttccagctca tctgctatct cgtccgcagt ttcatcagtt gtttcttcca
      361 aagcttcatt tgatacatct tctgctgcat catctacatc atcatcgtca tcatgatagt
      421 ccattgcctt atattttctg tttttcacag ctgatacaat aagcaacagt aacagaataa
      481 cgataataag aaccagtatt gcagctaaaa caagatattt attggcaatt cccgggacaa
      541 gctcgctctt tgttgtatcg ttttcctcct gtgccactgt aggtgtactg agctcggttc
      601 tcatatatgt gccatctacg ctatcatatg tataccagcc actctcacca ttgtcattta
      661 tggcatatac gaggtaaaaa tcctttgtct gatcatcggt tttttcggct ttggtctggt
      721 aggctgttgc cacacccttg ccctctatgg agagcgatat ctcattatat ccctcaggca
      781 catcatccac cttaggcagt aaaaccacta taaaatgcga ctctgaattg atacggacaa
      841 actcatatgc tgcattctgt gctgcatcat atacaaaaag cccattgccg ctttccgact
      901 gtaaatacac aagcgtgata tcaccaaagg tgccatgaag ggtattgtat tcaagtccgt
      961 caatcactgt ctttgagtgc tcaaagcctt caggaacaac atcctccgga atgtcattga
     1021 ccatagtcca ctctgtgcca tttatatcga acttcttcca gttatcctgc ttatctgcat
     1081 tctccggatc atcctcggct cttctggtaa caactatatt gtatgtgctt gtgctgccgt
     1141 cctctgctgt cacaacaacg ctgactgtgt tctctccaac ctcaagtgaa tcattgcctg
     1201 tgacggatgt cacactcgcc ttggaatcag ccggatttgc tgtaaccgca aggcttgaac
     1261 agctgtaatc tactgtagct gtgtaatcct ttgtcgcagc agaaaactca ggggaaagcg
     1321 tacctgctga gataacaagc gaaccaagag atgcgtttga ggatttcttt tcctctttgt
     1381 tttcattatc tttattcgca ttgttgtcat tgccggtatt gtccttgtta tccttgttgc
     1441 tgtttgaatc attgtttgat gaagcacttg ttaccgtcac cttcgtgctg gcaccggtaa
     1501 ttgtacctcc ggcatcgttg ccctctgcat ctcctacaac tgtacaatat gcctttatag
     1561 tgcagcttcc tgcagccttc gccgtaaatg aaaatgactg cgatgtggga tatcctgcgg
     1621 cgtcaccaat attggccaca ccatctcctc cgttactcag tactccctcg tcagagcttg
     1681 agataactac agtaccaaaa tatccagacg gcatggaaac agtcacctta actgtttttc
     1741 caacagctat agagcttgag ctgacactga gaccaagtcc ctgcgcatat acatgctcgg
     1801 caggtgtgaa aacaagcatc accgccacaa caagtgataa tatacatcca aataatttct
     1861 tcatatcttt cctcacattt ctaattctgc gatatctttg atttgcccag tatgtgtctg
     1921 ttcatgatta tcatatcaag ggcatttcca ccatcaccct ttctgttagc atcagctgcc
     1981 tccagataag cacctgtgag tgttcccttt cctaagatat gtctgttgag cttaatcata
     2041 tcaagagcat ttacagcacc atcaccattg atatctccat atataacaat atcgtatgtc
     2101 tttttcagat tacctgaagc atcataaaca gccactttat tgcctgttgc aatctttccg
     2161 ctgttttctg agcctgagct tgtcagaagc tttacagtac ctgatgctga tactgctata
     2221 ttcttcacaa aatcagctgc accggttcca ggctcaatgc ctgtaatata agtacctata
     2281 ctgtatttac ctgatgttat gtttacatca gtattatttt ggttattatt gttctgatta
     2341 ttattgccac ctgcattatt tgcagatgcc gcctgcctgt ttatattgat agtgtatgtt
     2401 cttgtatcac cgctttgcga cttgcatttt accttaattg tattatttcc gacagcaagc
     2461 gaatagcttc ctgtgcctga aacaccactg tttcctgaaa ctgcatctgc cgaaacagtc
     2521 actgagctta tagcatttga aacaactgct gaatagcttg ttgtcgttcc attgaaggcc
     2581 ggtgtgagtg ttacaccgct tatcgcaagc gactgcagat agttattggt atcacctgct
     2641 ttattgaagc ctactgctga cgatggcata ttattgtata caggtatttt aaatacaaaa
     2701 gcctgattct tgtctgaata tcctttagcc agactctgtc cctcagtaag agccgctgtg
     2761 gcattgccca tgtactgatg gaaatacagc gagcttgtat ttactacatt aaacttctca
     2821 aaatacagtg tattttgacc cttatttata taattgctgc ccacctttac agctccgccg
     2881 cttatggact ttactcttgt attccagccc tgcttcttgg caaaagacag accatttttn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctctttgcca catccatgaa tgtgcttgca
     3121 taattgattt tctcaccatt gttggaatag gtctttgttc cctccataaa tgtgcctttt
     3181 actatggact tgacaccttc aagtgcctca ttcggactgt atgcaaggct ttggaactgg
     3241 aaaatattgg tctcatcaag gaagttcctt ggatccatga gataagcaat ataagcactg
     3301 cttgctgaca cccagcccgg ctcatactca acccacgtat ttgttgacca gttatatgct
     3361 ccggcagctg tggatttctt ggcattgtct gctgatttgg ataccagatt aagtccgttt
     3421 acactctcat tttcaatcac cgtaccccaa ttcaaacctg tatttacagc ctcaaatgtc
     3481 cagcttggat acttattgtg aagagctgta aggtttggaa tatagctatc aggaaagcct
     3541 gcattcttca tagccgtgac aaaatcagta ttgtctccgc tattattgta tgtcaccttt
     3601 atgtatgtac tgatagtcca gcccgtaaat gccttgccgt ttaaagtacc cttgacattg
     3661 taccagacca caccgtttac cctggtctct cctattatgt caacctgatg gttccagttg
     3721 agtgagacaa ccttggtagt gccggatgtg cctgctgcat tgcggacata cacattgcca
     3781 cctgtaatcg tacctgtagc atatgctccc gatgcggttg caagggcaag ctgttcctca
     3841 ttattctgct gtatggcatc cgcattgccg gtgtctgccg aagcatcccc tggctgcgca
     3901 ttgtctgatg cagctgtgcc ggtacctgct gatacaaaat cagacctgat atagctgaca
     3961 cactcctcgt tatttgctgc cagattgtac tttgccttgt accacacctt gccgtctgca
     4021 cctgtggtct gctcaagaat agtaatatcc ttgccgtttg aaagcttgtg ctccagctca
     4081 tactctgtgc ccggtccgct tctcgccgct acccaatatc tctcatcaca cacaacctta
     4141 gccgcctgat ccgcatagac tgctatgctg ctgtatggtg ccaggtcaca tgcggctaat
     4201 gacaatgtaa cgacagaaac tgccagaaat ttatttatct ttatcatcaa atcccatctt
     4261 tcttatgtgc attgtttaga ttaatccatt atccatttaa ttataataga atatgacctt
     4321 tgtatcaatg tattcggcag aaatttatta tttttttatt aattaaaaaa actcccttca
     4381 acgtgcagct catgcttacg tttcagggag ttgcctgatt catattctgc ttattcttac
     4441 tgtctcctgt agcttttcta gctgaacagg gattttatct tatcccataa atccatgaaa
     4501 aagttaccaa gcttttccca gaagcccggg tcatttatct tatcgccaag ctttgatgcc
     4561 cagtcctgtg cctggttgat gactgaatcc cagtcgatat tggcatcctt aagcttcata
     4621 agaagcgatg tcagctttgc aatatcatca tcagaaagct tcaggtcgta atcctttgca
     4681 gcctccttta tcgcatcctt tatctggctc tcatccttaa gccctccatc agcaagctgc
     4741 ttctttaaat ctgcaatcat tgcctcgata tcctgcgaat cgccgtcaat gctcttgtta
     4801 agctctcctg ttgtgacaag ctcatccatc gctgcgtctg tcaccttgtc gtcaagcttc
     4861 ttgcctgtca tctcctcgta tgcctttagt gtgcctacaa gtgctgccgt tccggaaata
     4921 gggaacggtc ctgcgactat gattttagca tcagctatgc ctgctgtggc aagcgcattc
     4981 ttgtacattc ctaccgtaca gtaattgata ttgtgcgtgg aaacactaag acccgcaccc
     5041 tttttatcct ctgtaatgag tactgaggaa agtgaccttg tgccaatctc gctcttgctg
     5101 atgtatgaat cgaggtactt gtgctcctca tcattattta catagaccac atcgtatttg
     5161 tccagatcag tgagctcgat tcccataaat ccaagcactg tcgctctctg atccggtgtg
     5221 aggtctgctc caagacttaa gtatggctta tcattnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnngtaaaca gtgtcaccgc agcaagcacg ctacatacaa tactctttgc ttttttcttt
     5461 attaatctaa tcatttttcc tccaatctac tgctctatat catacgaaat tgtaaagtta
     5521 cttccgtcga agctgacatc tgccatactt ccgcatacca caggcatcat cggcgacata
     5581 tgacctatat ccagatccat gatgattggc acattaaact ctctaagcag tgatgaaagc
     5641 gcctgtctgt ggtctatgcc aaacatttcc tctccgaagt gcgccgggcg tccgataaga
     5701 aagcccttta catttgaaaa ccatcctgca tttttcatct gccacatagc ccttctcatg
     5761 cccatcacat taaggtcaca agcctcaagg aaccatatga taccatcatc actgtagcgc
     5821 tcattaaatt cccttacatg gtcaaacgat gtaccaagca gattgataag acaatccatg
     5881 cagccaccaa gcagcctgcc tctcatggta agcctgcttc cctttgccac cgcaggaata
     5941 gagctgatgc acgacttttc agtcaggttg tagcccacaa acggagcatt ctcatccttt
     6001 atcgattcct tctcccacat agggtaattg gtaaatgaaa gcttcctgcc acaaagcaga
     6061 ttaaatgcat cggtaagtga ctcatgccac acctccatac caaagctgcc cgcacacggc
     6121 ccgtaaatac ctgctgtatc tgcaatagta gcctccaaaa atgtgaaatt cgtgttgtcg
     6181 gaatagccca tgtaccattt cggctttgac tgcttcacgc gctcaaaatc aatataaggc
     6241 actacctcgc acatcagctc gccaccacca caggatataa gcacatcact gtcagcagaa
     6301 gcatagctct cgttcagctc ttctgcgcac ttcttaggtg tattgctgat acctatgcca
     6361 ctgtcgcacc tgcagttagg tccctctatc gtcttataac ccatgctgtg aaacctttta
     6421 atggcattgt caaaggcact cctgtacggc tctgttgtac agcccatcga gggcgccaca
     6481 aagcctattg ttcctctatc ctttagaaat tcagggtatc tcatgtttct atcatcctat
     6541 cactattatt tacatatcta agcttctgtc tgaagtaaaa tatgtaggat tttatcattt
     6601 ctgtcaaggt cattttactg tcataattaa atgtcgtcgt gtactgcaca tgctgtctgt
     6661 tgcaccagtt gtatatatca tgcagactta tcgaatcaac atgcttcata ttgttcataa
     6721 acgccttaac ctttaaaagc tcatcctcac tgtcaatgta tagatgaaag atagtattcg
     6781 gacattcaat tattatctgc tgttcttcta tatttttcat cactgtatcc tctattgaat
     6841 aatgtatcct gtgtgattaa aaacattatc gtctgtcaca ccgggatgtg agtaataaca
     6901 aaatatcgta actgttcagt gccttcacag ttaaaaaata tcacataacc atagtactac
     6961 accggcgtca caaaatccag tcatttttgc ctgccaattc ttactatttt attaagaaaa
     7021 acggtaaata tcatactccc tcacagtcaa atgccgggat aaagctgtcc tttgccacgt
     7081 ccccttcctg tataaatgca ttcgtgattc ctatttcgag ggcatagtca ataagccttt
     7141 catactctct ccttgtcact gtgcggttta tctcaggata atcctttagt gcaatcggcg
     7201 taaactggct catcatgctt atatatacgt gatcatgata tgtatcatac aggtacttaa
     7261 gtacagcctt tgcatcattc acatgtcccg gcagtagaag ctgtcgcaca ataactccct
     7321 tttgtatgag tcctgtcgca tcgtcaatca cgacatccgg ctgctgcctg accatctctg
     7381 aaagcgcctg ctttgcgaca gacggataat cttttgccct tgaatac
//
LOCUS       DPUF01000274            3031 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16647, whole
            genome shotgun sequence.
ACCESSION   DPUF01000274 DPUF01000000
VERSION     DPUF01000274.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16647"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="DHV81_09350"
     CDS             <1..1599
                     /locus_tag="DHV81_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HCI94630.1"
                     /translation="NNIEKTMTKAGYTDYTVADVPAESWIMTLLPYLLVFGAMFILFA
                     VMNNNAAAQGGGGKMMNFGKNRAKLTTQEENHIKFSDVAGLKEEKEEIAEIVDFLRDP
                     GKYTRLGARIPKGVLLVGPPGTGKTLLAKAVAGEAGVPFFTISGSDFVEMFVGVGASR
                     VRDLFEDAKKNAPCIVFIDEIDAVARRRGTGMGGGHDEREQTLNQMLVEMDGFGVNEG
                     IIVMAATNRVDILDPAIMRPGRFDRKVVVGRPDVGGREEILGVHAKKKPLAEDVDLKQ
                     IAQTTAGFTGADLENLMNEAAIRAAGENREYITQDDIRKSFVKVGIGAEKKSRIISDK
                     EKRITAYHEAGHAILFHLLPDVGPVYTVSIIPTGAGAAGYTMPLPEKDEMFNTKGRML
                     QEIVVDLGGRVAEELVFDDITTGASQDIKQATKLAREMVTKYGMSDNIGLICYADDEE
                     EVFIGRDLAHAKNYSEGIASAIDVEVKRIIDESYDKAKSMIAEYRDVLDRCAALLLEK
                     EKITRDEFEALFDEDSKTTVEHNV"
     assembly_gap    1746..1818
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2240..2321
                     /locus_tag="DHV81_09355"
     tRNA            2240..2321
                     /locus_tag="DHV81_09355"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:2274..2276,aa:Tyr,seq:gta)
     gene            complement(2378..>3031)
                     /locus_tag="DHV81_09360"
     CDS             complement(2378..>3031)
                     /locus_tag="DHV81_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94631.1"
                     /translation="KDKLSVALDSLEEILGCFPDDFFTQLCYGNIRGMRIYLCGDISS
                     GHSDMINEPSGFVNIINNHNVMVLNCNYSWDFSYTVGHEISHLIDQRLTFIHTYDEKS
                     EFSEGKWNSFNPDSFSYDDSYANYNPDDPSNHISGYFIDSYGMTFATEDRSEIFGTAI
                     DDYINGIYDDARFTDDSPIRNKLDYYSRCIRDGFDTKDWPDTMAWESVLTATGAQAD"
BASE COUNT          866 a          621 c          738 g          733 t
ORIGIN      
        1 aacaatatcg aaaagaccat gacaaaagca gggtacaccg actatacagt tgcggatgtt
       61 ccggctgaga gctggattat gacactgctt ccgtatcttt tggtattcgg agccatgttc
      121 atactctttg cagtgatgaa caacaacgca gcagcacagg gcggcggtgg caagatgatg
      181 aacttcggca agaaccgtgc gaagctcacc acacaggagg agaaccatat caagttttcc
      241 gatgtagcag gcttaaagga ggaaaaggag gagattgcag agatagttga tttccttcgt
      301 gatccgggaa aatacaccag acttggagca agaatcccaa agggtgtcct tcttgtagga
      361 cctccgggaa caggtaagac tctccttgcc aaggcagtag ccggagaggc aggggtaccg
      421 tttttcacca tttcaggatc tgatttcgtg gagatgttcg tcggagtcgg agcctcacga
      481 gtgagagatt tgtttgagga tgccaagaaa aatgcgcctt gtatcgtatt tatcgatgag
      541 attgatgcag tagccagacg acgtggcaca ggaatgggcg gcggacacga cgagcgtgag
      601 cagacattga accagatgct tgtcgagatg gatggctttg gtgtcaatga gggcattatc
      661 gtcatggcag ccacaaaccg tgtggatata cttgaccctg ctatcatgcg tccgggacgt
      721 ttcgacagaa aggttgtagt aggcagaccg gatgtgggcg gcagagaaga aattttaggt
      781 gtccatgcca agaaaaaacc tcttgcagag gatgtagact taaagcagat tgcacagact
      841 accgcaggct tcacaggtgc agaccttgag aatctgatga atgaggcagc aatccgtgca
      901 gcgggcgaaa acagagaata tatcacacag gatgatatca gaaaatcctt cgtcaaggtt
      961 ggtatcggag ctgagaagaa gagccgtatc atctctgaca aggaaaagcg catcacagcc
     1021 taccacgagg caggacatgc aatccttttc catctgcttc cggatgtggg acctgtatac
     1081 acagtttcaa tcatccctac aggagcaggc gcagcaggct acaccatgcc tctgcctgag
     1141 aaggatgaga tgttcaacac aaagggacgt atgcttcagg aaatcgtagt agacttaggc
     1201 ggtcgtgtcg cagaggagct tgtgtttgac gatatcacaa caggtgcatc acaggacatc
     1261 aagcaggcta caaagcttgc gcgcgagatg gttaccaaat acggtatgtc agacaatatc
     1321 ggccttatct gctatgctga tgatgaggag gaggtcttca tcggacgaga cctcgcccat
     1381 gcaaagaact acagcgaggg catagcatcg gcaatcgatg tcgaggtaaa gcgtatcatc
     1441 gacgagtcgt acgacaaggc aaagagcatg atagcagagt accgcgatgt gcttgacaga
     1501 tgtgcagcac ttcttcttga gaaggagaaa atcacaagag atgagtttga agcactgttt
     1561 gacgaggaca gcaaaacaac agttgaacat aatgtttaaa aaatagctat acttttgtgc
     1621 agaatgacta tatgaaaaat attgaatatg actaaagaac tatacaaatt gcataaaaac
     1681 aaaccgcgat ttttatgcaa tttgtgaaca cttatcgtta taaccgtgat attataataa
     1741 ccgtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnta aaaatacctt ctcctaaaag gcagccgatc gtaggctgtc
     1861 ttttttaatt tctatgttat agctacaaag taagggattg tgtgcccggg atggtgcttt
     1921 ggtgggggat tgatgccctt actggagcgc atagcgaagt tgcttagagt ctgtcagaat
     1981 tatgcttaga ggagcagaaa tgcccggcca tggacggcgc ataaaagccg gtttcgtcag
     2041 tcatagaaag attaaaagca taattcctac agactcttag cagcgtagcg tagcgaaagc
     2101 gagggcatca atcccccacc aaagtatcgt ccaaggcgcg caatccacgt attaaagaaa
     2161 ataatttggt tacgataaaa tatgcttgca taatcacatc tgttgtgata aactatgcaa
     2221 gtatcgtaat taaatacatg ggcagattcc cgagtggcca aaggggacag actgtaaatc
     2281 tgctgcaact tgcttcgatg gttcgaatcc atctctgccc attaaaaaac ctcaagtcgt
     2341 atgacttgag gtttttgtgt tattctgaaa ttatcattta atcggcctgc gccccggtgg
     2401 cggtaagcac agactcccat gccatcgtat caggccagtc ctttgtatca aagccatcac
     2461 ggatacatct gctgtagtag tccagcttgt ttcgtatcgg cgaatcatct gtgaatcttg
     2521 catcatcata gatgccgtta atataatcat ctatcgcggt tccgaatatt tcagagcggt
     2581 cctctgttgc aaaggtcatg ccgtaggaat caatgaaata cccggatatg tggttcgatg
     2641 gatcatccgg attgtagttt gcgtagctat catcataaga gaagctgtca gggttaaagc
     2701 tgttccattt cccctcagaa aactcgctct tttcatcgta ggtgtgtatg aatgtaagcc
     2761 tctggtcaat cagatgggaa atctcatggc ctacagtgta gctgaaatcc cagctgtagt
     2821 tgcagtttag aaccatcaca ttgtggttgt ttattatatt tacgaagcca cttggctcat
     2881 ttatcatatc tgagtggcct gatgaaatat caccgcacag atagattcgc atgcctctta
     2941 tattgccata acacagctgt gtgaaaaagt catcagggaa acagccaagt atttcctcaa
     3001 gcgaatccag agcgaccgac agcttatcct t
//
LOCUS       DPUF01000275            3770 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_14060, whole
            genome shotgun sequence.
ACCESSION   DPUF01000275 DPUF01000000
VERSION     DPUF01000275.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_14060"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="DHV81_09365"
     CDS             <1..642
                     /locus_tag="DHV81_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI94632.1"
                     /translation="EKDYLELSGFEVEVENDGTSGLARALAEDFDLFILDLMLPGIDG
                     FEICKRIREKKNTPILMVSAKKDDIDKIRGLGLGADDYVTKPFSPSELVARVKAHLAR
                     YERLIGSATPENDIVEIRGIKIDKTARRVWVNGEEKQFTTKEFDLLTFLAENPNHVFT
                     KEELFKEIWNMDSVGDIATVTVHIKKIREKIEMNTAKPQYIETIWGVGYRFKL"
     gene            766..2265
                     /locus_tag="DHV81_09370"
     CDS             766..2265
                     /locus_tag="DHV81_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI94633.1"
                     /translation="MKIKTKLAISFCIIIFVPVVLTSIVLVGFNKIQLKAINKTYGME
                     DAGMLALTDTVQFLNKVTGRTYDELEKTSLIEPSRLLDSDYLTKINKKLEKKYSYLIV
                     KSEGELIFNGGIDNDDILRKLPRISNKQSSSDVSSYMDSDDKVLIKQLNFRDSDGDEA
                     SLYIVTSTACVIPEVRTVLIEGAFALVLILLTTAAMLIVWIYRSIITPIERLRIAAEN
                     IKDGNLDFELETSPSDGEIGELCTAFEQMRARLKKNAEEKVTNESENRVLISNIAHDL
                     KTPITAVKGYAEGILDGVANTPEKVDKYVRTIYNKANDMDKLINELTLYSKIDTNRIP
                     YNFAKINVTDYFNDCVEEIGLDLEAKNIRLSYENHVDSNVLIIADPEQIRRVINNIVG
                     NSVKYMNKTQSYIDIRINDVGDFIQVEIEDNGRGINQRDMPYIFDRFYRADASRNSAT
                     GGSGIGLSIVKKIIEDHGGKIWATSKEGDGTTMFFVIRKYQETQDMNRQ"
     gene            2578..2787
                     /locus_tag="DHV81_09375"
     CDS             2578..2787
                     /locus_tag="DHV81_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94634.1"
                     /translation="MPMKPRNVQPWTAGQGVMRHGRRIKAGFVKHRKTPRHNSYRILA
                     AKRSGSESVACPPGLCDLRPAPWPL"
     gene            2914..>3770
                     /locus_tag="DHV81_09380"
     CDS             2914..>3770
                     /locus_tag="DHV81_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI94635.1"
                     /translation="MHKFRQKYIGDKAFYKRYLMLAIPMIIQNAITNLVSFLDNIMVG
                     QLGTEQMSGVAIVNQLIFVYNLAVFGAVSAASIFGAQYFGKGNHKGHMYSFRFKLYAA
                     LLVTAIAMTLFITNGTGFISLYLTDTAGNGATEVALAYGKEYLEIVMIGLIPFAVTQA
                     YATNIKETGQTFVPMIASFAAVGSNAVLDFLLIFGIGPIPKLGVAGAAIATVMSRYIE
                     TIIVVLWAHKNRDKNRYLEGAYRGIGMPFGEFKTIFIKGLPLMLNELFWAAGMTTVTQ
                     CYSVRGLSV"
BASE COUNT         1207 a          674 c          947 g          942 t
ORIGIN      
        1 gagaaggatt acctggagct gagcggcttt gaggtagagg tggagaatga tggcacaagc
       61 ggtctcgcaa gggctttggc ggaggatttt gatctgttta ttcttgacct tatgctgccg
      121 ggcattgatg gctttgagat ttgtaagcgg attcgtgaga aaaagaacac accgatactc
      181 atggtttcag caaagaagga tgatatcgac aagataagag gactcggact cggcgcagat
      241 gattatgtca caaagccgtt ttcaccatca gagcttgttg ccagggtaaa ggctcatctg
      301 gccagatatg agcgcctgat cggaagcgcc acacctgaaa atgacatcgt tgaaattcgt
      361 ggcatcaaga ttgacaaaac agcccgccgt gtgtgggtaa acggagagga aaagcagttt
      421 accacaaagg aatttgatct gctcacattc cttgcggaga atccaaacca tgtatttacc
      481 aaggaggagc tctttaagga aatctggaat atggattcgg tcggagatat cgcgacagtc
      541 actgtacata tcaagaagat tcgtgagaaa atcgagatga acactgcaaa gcctcagtat
      601 attgagacta tttggggcgt gggatacaga tttaagctgt agtgattttt aaaaaatata
      661 gtagatattg tagtattaat aggacatatc aaattcaaat gcgaaaggat atgtccttgt
      721 tgtatatata aagtggctta ttaatttatt caaatggaga atactatgaa gataaaaaca
      781 aaacttgcta tatcattctg tataattatt tttgtgcctg ttgtgctcac cagtatagtg
      841 cttgtgggct tcaataagat tcagctaaag gccataaaca agacatatgg catggaggat
      901 gcgggcatgc tcgcgctcac tgacacggtg cagtttctga acaaggtgac agggcgcaca
      961 tatgatgagc tcgagaagac atcgctcata gaaccatcca ggcttcttga tagcgattat
     1021 ctgactaaga taaataagaa gctggagaag aagtattcat atctcatagt gaaaagtgaa
     1081 ggagagctca ttttcaatgg tggaattgac aatgatgata ttttgagaaa gcttccgcga
     1141 atatccaata agcagagcag ctctgatgtg agcagctata tggacagtga tgacaaggtg
     1201 ctcataaagc agcttaattt tcgtgacagt gatggtgacg aggcgagtct ttacatagtg
     1261 acatcaaccg cgtgtgtgat ccctgaggtc aggactgttt tgatagaggg agcatttgca
     1321 cttgtattga tacttcttac tacggctgcc atgcttatag tgtggatata caggagtatc
     1381 atcacaccga tagagaggct gcgtattgcc gctgaaaata tcaaggatgg caatctggat
     1441 tttgagcttg agacgagccc gagcgacgga gagattggcg agctgtgtac tgcgtttgag
     1501 cagatgcgtg cgcgcctcaa gaaaaatgcc gaggaaaagg tgacgaatga gtctgaaaac
     1561 agagtgttaa tcagcaatat cgcgcatgat ttaaagactc ctattactgc ggtaaagggc
     1621 tacgccgagg gcatacttga cggagtggca aacacccctg aaaaggtaga taaatacgtc
     1681 agaaccattt acaataaggc aaacgatatg gataagctca ttaatgagct gacactttac
     1741 tcgaagattg atacgaacag gattccatat aattttgcga agataaatgt cacagattat
     1801 tttaacgact gtgttgagga aataggactc gatcttgagg caaaaaatat caggctttca
     1861 tatgagaatc atgtggatag caacgtgctt atcattgctg atccggagca gattcgccgt
     1921 gtgattaata atatagtcgg caattcggtg aagtatatga acaagacaca aagctatata
     1981 gatatcagga taaatgatgt tggtgacttc atacaggttg aaatcgagga caatggacga
     2041 ggaatcaacc agagagatat gccatatatt tttgacagat tttacagagc ggatgcgtcg
     2101 agaaattcgg ctaccggagg cagcggcata ggactttcca tcgtaaagaa gattatagag
     2161 gatcacggcg gcaagatatg ggctaccagc aaagagggtg atggaactac catgtttttc
     2221 gtaatcagaa agtatcagga aacacaggat atgaaccgac agtaaacaaa taaacaggat
     2281 attgacataa aaagtaaagc tataattata acagttagat attagaattt aaaattaaga
     2341 gctcccgatt gacaacgcaa atagtgttgt cagtcgggag ctttaattat tggaaaatag
     2401 tactgttatt atacaatagc ttcgatataa tagttttcgg tgccgtttat aataggacgc
     2461 agatataata aaaattaaaa ccaaccacaa cgtaaggggc gtaagtcaca gagtccgggt
     2521 gggcatgcca cgctctcgcg gacgcgaata gcggagcagc ttagaagctg tagaattatg
     2581 cccatgaaac ccagaaatgt ccagccatgg acggctgggc aaggcgttat gcgccatgga
     2641 cggcgcataa aagccggttt cgtcaagcat agaaaaaccc caaggcataa ttcgtacaga
     2701 atcttagctg cgaagcgtag cggaagcgag agcgtggcgt gcccacccgg actctgtgac
     2761 ttacgccccg ccccatggcc attatgatac aaaattctga atcctaaaat aagttaagga
     2821 aaaacacata aaatatttga aaaataaagt ctgacgtagt agaatagtaa cattgcacga
     2881 aaggaccatg gagaaatcga aagggggaca aacatgcata aattcagaca aaaatatata
     2941 ggagacaaag cattttataa gcgctatctc atgctggcca taccaatgat aatccagaat
     3001 gcaatcacaa atctggtaag ctttctggac aacattatgg taggacagct tggtacagag
     3061 cagatgtcag gagtagcaat cgtaaaccag ctcatatttg tgtacaatct ggctgtattt
     3121 ggggcggtgt cagcggcaag tatattcgga gcacagtatt ttggcaaggg aaatcataag
     3181 gggcatatgt attccttcag attcaagctg tatgcagcgc tgcttgttac agcaatagcg
     3241 atgacactgt ttatcacgaa cggaacaggt ttcatatcac tttatctgac agatacggca
     3301 ggaaacggtg ccacggaggt agcactggca tatggaaagg aatatctgga aattgttatg
     3361 ataggtctca taccgtttgc ggtgacacag gcctatgcga caaacataaa ggaaacagga
     3421 cagacttttg taccgatgat tgcaagcttt gcagcagttg gaagcaatgc ggtgcttgat
     3481 tttctgctta tctttggaat aggaccgata ccaaagctgg gtgtggcggg agcagctatt
     3541 gccactgtga tgtcgcgata tattgagaca ataatagttg tgctgtgggc acataaaaac
     3601 agggataaga acagatatct cgagggcgct tacagaggaa taggaatgcc gtttggtgag
     3661 tttaagacta tattcatcaa gggactgcca ctcatgctca atgagctttt ttgggcagca
     3721 ggcatgacaa cggttacaca gtgctattca gtacgtgggc tctcggtaat
//
LOCUS       DPUF01000276            2529 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_13558, whole
            genome shotgun sequence.
ACCESSION   DPUF01000276 DPUF01000000
VERSION     DPUF01000276.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_13558"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="DHV81_09385"
     CDS             complement(<1..1154)
                     /locus_tag="DHV81_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydrofolate--homocysteine
                     methyltransferase"
                     /protein_id="HCI94636.1"
                     /translation="MTREEFRKYIRENIVILDGATGTNLMAAGMPVGVCPEEWVMEHP
                     QVILDLQKAYVDNGTNIVYAPTFTGNRIKLLEYGLQEKINEINTTLVGLSKQAVGDRA
                     LVAADMTMTGRQLFPLGDLMFEELLEVYKEQARILDAAGADVFVVETMMSLQECRAAV
                     LAIKEVSDLPIMVTLTYNEDGRTLFGTPPETAVVVLQSLGVDAIGVNCSTGPAEMIAL
                     VEKMAEYSTVPLIAKPNAGLPELEDGKTVYKMTPDMFADAMRGLVKAGASIVGGCCGT
                     TPEHIRALTEAVKSMPVHKPLEHHRRVLASERKNVEIDLDGNFTVVGERINPTGKKKL
                     QAQLREGKLDLVRQMAMEQETNGAGILDINMGMNGIDEKEMMKSVIYEVSS"
     gene            1354..2175
                     /locus_tag="DHV81_09390"
     CDS             1354..2175
                     /locus_tag="DHV81_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI94637.1"
                     /translation="MKLIALDLDGTLFNNKSQISKENIKAIKEATAAGINVVISTGRP
                     FGGLPFEAIKDTGIRYAITANGSAIYEIDTQKCLYENCLSDETAFSIIDYLMTKRVHM
                     DAFINGCGYSPYKCLEDAKDLKMPPSIKEYIMTTRKRVNDITEMMRENNYHMQKMTIN
                     FPKDVDDNYYCRDEVFEHLKKIPEITLVSGGYGNLEFTKAGVDKGVALXXXLNIAADE
                     TMAVGDTENDMAIVKAAHIGVAMGNATDELKAQADYVTDTNENDGVAKAIRHFMG"
     assembly_gap    1977..1986
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2312..>2529
                     /locus_tag="DHV81_09395"
     CDS             2312..>2529
                     /locus_tag="DHV81_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HCI94638.1"
                     /translation="MTGDFLIVKKYLSNPLVTGTIFLTLAGTTSRFMGFFFRIFLNNV
                     MGSTGLGLYQLVIPLMSVCMSLCCNGFQT"
BASE COUNT          720 a          641 c          470 g          688 t
ORIGIN      
        1 gatgaaacct catatataac cgatttcatc atctccttct catcgatacc attcatgccc
       61 atattgatat caagtattcc cgcaccattt gtctcctgct ccatagccat ctggcgcacc
      121 aggtcaagct ttccctcacg aagctgtgcc tgcagctttt tcttgcctgt agggttgatt
      181 ctctctccga ccacggtaaa gtttccatct aagtcaatct ctacattctt tcgttcggat
      241 gcaagcactc ttctatgatg ctcaagcggc ttatgaacag gcattgactt tacggcctct
      301 gtgagtgcac ggatatgctc cggcgtggtg ccacagcagc ctccgacaat ggatgcacct
      361 gccttcacaa gccctctcat cgcatcggca aacatatccg gagtcatctt gtatacagtt
      421 ttaccatcct caagctccgg aagcccggca tttggctttg caataagagg cactgtagaa
      481 tactctgcca ttttctcaac cagcgctatc atctcagcag gtcccgtcga gcagttcaca
      541 ccgatagcat ccactccaag tgactgcagc acaacaactg ctgtctccgg cggtgtacca
      601 aaaagagtcc tgccatcctc gttgtaggtg agtgtcacca taataggcag gtcgcttacc
      661 tcttttatcg caagcactgc cgctctgcac tcctgaagac tcatcattgt ctcaaccaca
      721 aaaacatctg cgcccgccgc atcaaggatc ctcgcctgct ccttgtacac ctccaaaagc
      781 tcctcaaaca tcagatcccc gagtggaaaa agctgtctgc cggtcatcgt catatctgct
      841 gctacaagtg ccctatcccc gacagcctgc tttgacaggc caacaagcgt ggtgtttatc
      901 tcgttaattt tttcctgtaa accatattct aaaagcttaa tcctgtttcc tgtaaatgta
      961 ggtgcataaa caatattcgt gccgttatcg acataagcct tctgcaaatc aagaatcacc
     1021 tgcggatgct ccatcaccca ttcctccgga cacacaccta caggcatgcc cgctgccatc
     1081 aggtttgtac cggtcgctcc atccaggata actatgtttt ctctgatata ttttctgaat
     1141 tcttctcttg tcatttcgtt tccttttctg tttcagctgt tattcactcc acagtgtaaa
     1201 atatttcaaa gcaaaagcta tgtgctcttc atccttgaaa tttgtgaaaa tttatattat
     1261 cattgtaacc gattttaaaa cacaaaaatc aatagtctat tcaccataat atactaaaac
     1321 actatatttt tcaaacaaaa gaaaggattt cttatgaaac ttattgcttt agatcttgac
     1381 ggaacacttt tcaacaacaa aagtcagata tccaaggaaa atataaaagc tataaaagag
     1441 gcaactgctg ccggaataaa tgtggtcatc tccaccggcc gtccattcgg agggctgccg
     1501 tttgaggcaa ttaaggacac cggcatccgc tacgctatca ctgcaaacgg aagtgcaatc
     1561 tacgagattg acacccaaaa atgtctttac gaaaactgcc tttctgatga gacagcgttt
     1621 tcaataatcg attacctgat gacaaagcgc gttcatatgg atgccttcat aaacggctgt
     1681 ggctattctc catacaagtg tctcgaggat gcaaaggatc tcaaaatgcc tccttctata
     1741 aaggaataca tcatgacaac cagaaaacgt gtgaacgata tcactgaaat gatgcgcgaa
     1801 aacaattacc atatgcagaa aatgaccatc aatttcccaa aggatgtaga tgataactac
     1861 tactgccgcg acgaagtatt tgagcatctg aaaaaaatcc ctgaaatcac gctcgtaagc
     1921 ggtggctacg gtaatctgga attcacaaaa gcaggcgttg acaaaggtgt ggcactnnnn
     1981 nnnnnnctga atatagcagc tgatgagaca atggctgtcg gtgacaccga aaacgacatg
     2041 gctatcgtaa aagccgcaca catcggcgtg gccatgggaa atgcaaccga tgagcttaag
     2101 gcacaggctg attacgtcac cgacacaaac gaaaatgacg gcgtagccaa ggctatccgc
     2161 cactttatgg gttaatctca caaatttaat ataacgatac cggtcatata agagtataat
     2221 agttgtttag cgcaaaatag ctgtttaaca cacaataaat aatcacctgt catatatatc
     2281 caaagaagca catatacttc taagacaaca tatgacaggt gattttttga ttgtaaaaaa
     2341 atatttatcc aacccacttg tgaccggaac tattttcctg acacttgccg gtacaaccag
     2401 tcggtttatg ggttttttct ttcgcatatt tcttaataac gtcatgggct caacaggtct
     2461 gggactctat cagcttgtga ttccactcat gtccgtatgc atgtcactct gctgcaacgg
     2521 ctttcagac
//
LOCUS       DPUF01000277            6659 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_11051, whole
            genome shotgun sequence.
ACCESSION   DPUF01000277 DPUF01000000
VERSION     DPUF01000277.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_11051"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="DHV81_09400"
     CDS             <1..855
                     /locus_tag="DHV81_09400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742718.1"
                     /note="catalyzes the dehydration of
                     2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate
                     in valine and isoleucine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCI94639.1"
                     /translation="AIAHEIGFDFDISFANPISERTPNLCHLAPAGPTYMEDLNEAGG
                     VWAVMKELADIGLLNTDCMTVTGKTVGENIKNAVNRDPEVIRPVDNPYSKTGGLAVLK
                     GNLAPDGSVVKRSAVVDEMMVHEGPARVFDCEEDAIAAIKGGKIVEGDVVVIRYEGPK
                     GGPGMREMLNPTSAIAGMGLGSSVALITDGRFSGASRGASIGHVSPEAAVGGPIALVE
                     EGDIIKIDIPNMKLELDVSDEVLAERKAKWQPREPKVTTGYLKRYAALVTSGNRGAIL
                     ALPGEQNA"
     gene            941..2629
                     /gene="ilvB"
                     /locus_tag="DHV81_09405"
                     /pseudo
     CDS             941..2629
                     /gene="ilvB"
                     /locus_tag="DHV81_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739007.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
     assembly_gap    1861..1930
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2631..3713
                     /locus_tag="DHV81_09410"
     CDS             2631..3713
                     /locus_tag="DHV81_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCI94640.1"
                     /translation="MSRVYNFSAGPAVLPEEVLQEAAAEMMDYKGSGMSVMEMSHRSK
                     WFDDIIKDAEKDLRELMNIPDNYKVLFLQGGASQFFAEVPMNLMKNKKAGYIITGQWA
                     KKAFAEAKIYGDAVELASSADETFSYIPDCSDLPITDDMDYVYICENNTIYGTKYKKL
                     PNTKGKNLVADVSSCFLSEPVDVSKYAVIYGGVQKNVGPAGVVIAIIREDLITDDCLP
                     GTPTMLKWKTQADNDSLYNTPPCYGIYICGKVFKWLKKMGGLSVMKERNEEKAKILYD
                     FLDQSKLFKGTVRKEDRSLMNVPFVTGDAELDAKFVKEATAAGFVNLKGHRTVGGMRA
                     SIYNAMPKEGVEKLVEFMKKFEEENA"
     gene            3713..4876
                     /locus_tag="DHV81_09415"
     CDS             3713..4876
                     /locus_tag="DHV81_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HCI94641.1"
                     /translation="MFNYTCLNPIAGVGLDLFSDDYKKVDDIKDADAALVRSAAMHDM
                     ELGDKVLAVARAGAGVNNIPLDKCAEQGIVVFNTPGANANGVKELVFAGMLYASRDIV
                     GGIDWCLANQNDENIAKTAEKQKKNFAGTEISGKKLGVIGLGAIGVLVANAAVHMGMD
                     VYGYDPYISVNAAWNLSRSVKHISNVEDIYKNCDFITIHVPLLDSTKKMVNADAIAMM
                     KPTAIVLNFARDLLVDEEAMVDALAKGKVKKYVSDFPNNTTVGAKGCIVTPHLGASTA
                     ESEDNCAVMAVKELRDYLENGNIVHSVNFPDCSMGACTASGRVGILHKNVKGMIGQIT
                     TALAEADINVSDLTNKGKGDYAYSLLDLDSAIDASTVEKLSAIDGVLRVRVIK"
     assembly_gap    4958..4988
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5018..5929
                     /locus_tag="DHV81_09420"
     CDS             5018..5929
                     /locus_tag="DHV81_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein"
                     /protein_id="HCI94642.1"
                     /translation="MIRQILGEVSVPVANTEVQQVIENPGIVKTYLEKYMPSLISFLV
                     QLVVAIVILLIGIKVIKSVVKIIKKGFDKSRMDAAVASFLANVIKYFLYFILVMALLS
                     GFGVATGSVIAVLGSAGLTVGMALQGSLSNFAGGVLILLMKPFSVGDYIVDGSSGTEG
                     TVKDINLFYTRLLTIDNKMVMIPNGKLADSCITNVSMMDKRMLDLRVTVSYSTDLAFA
                     KSVLESVVTSADTMLHDEPSNVFVSELQDSAIELGARIWVKNEDYWNTKWQLTEEIKT
                     AFDKNNIEIPFPQMDVNIQKRSIDSDF"
     gene            6049..>6659
                     /locus_tag="DHV81_09425"
     CDS             6049..>6659
                     /locus_tag="DHV81_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HCI94643.1"
                     /translation="MTIHDLAEYEILDEHRVEDVQSDGFILRHKKSGARIAILSNNDD
                     NKVFYIGFRTPPEDETGVPHIIEHTTLCGSKKFPVKDPFIELAKGSLNTFLNAMTYPD
                     KTVYPVASCNDQDFKNLMDVYLDAVFNPNITKYEEIFKQEGWHYELTGKDDELKINGV
                     VYNEMKGAYSSPDEVLSSQIYRSLFPDNTYSKDSGGNPEYIPKL"
BASE COUNT         2014 a         1197 c         1651 g         1696 t
ORIGIN      
        1 gctatcgctc atgagatagg ctttgatttt gatatcagct ttgcaaatcc aatcagtgag
       61 aggacaccaa atctctgcca ccttgccccg gcaggcccta catatatgga ggacttaaac
      121 gaggccggcg gtgtgtgggc tgtcatgaaa gagcttgctg atatcggact tttaaataca
      181 gattgtatga ctgttacagg aaagacagtt ggagagaata taaagaatgc agtcaataga
      241 gaccctgagg tcatcagacc ggtagacaat ccatattcaa agaccggagg actggcagtg
      301 cttaaaggaa atctggctcc ggacggctct gtagtaaagc gttcagcagt tgtcgatgag
      361 atgatggttc atgagggccc tgcaagagta tttgactgcg aggaggatgc aattgcagca
      421 ataaagggcg gcaagatcgt agagggtgat gtagttgtca tcagatacga gggaccaaag
      481 ggcggccctg gaatgagaga gatgcttaac cctacatccg ctattgccgg aatgggactt
      541 ggaagttctg tagcactcat cacagacgga cgtttttcag gagcttcaag aggagcttca
      601 ataggtcatg tttcacctga ggccgcagta ggcggaccga ttgctctggt tgaagaggga
      661 gatatcataa agattgatat tccaaacatg aagcttgagc tcgatgtgtc agacgaggtg
      721 cttgctgagc gtaaggcaaa gtggcagcca agggagccga aggttacaac aggctatttg
      781 aagagatacg cagcgcttgt tacatcagga aacagaggag ccatccttgc acttccgggc
      841 gagcagaacg cgtagtatat attttgatag aaaattcagt acgaataagg attttaccta
      901 ttatcgtaaa ggattcgtat aagaaaaaga ggaatacaag atgcagttaa caggatcaca
      961 ggttattata gaatgcttaa aagaacaggg tgttgacaca gtattcggtt atccgggagg
     1021 agccatcctt aatgtctatg acgagctcta caagcatccg gagattaagc atgtgctgac
     1081 ctcacatgag cagggagcaa gccatgcggc cgacggctat gcgagaagca ccggaaaggt
     1141 tggtgtctgt ctggctacaa gcggaccggg cgcgacaaat ctggtcacag gaatttcgac
     1201 agcatacatg gattcgattc caatcgtcgc gatcacatgt aatgtcggag tctcgttact
     1261 tggaaaggac agctttcagg agatagatat tgccggaatt acaacgcctg taaccaaata
     1321 cagctttatt gtaaaggatg tagagaagct tgcagataca atacgcagag ctttcaatat
     1381 agcaagaaaa ggcagaccgg gtcctgtgct tgtggatatt ccaaagaatg ttacggctga
     1441 tctggtagag tacaccaggc aggaaatcaa gcttcctgag cgtgtgacag aaacaattac
     1501 agacagtgac attgataatg caattgaact cataaaagca tccaaaaagc catatatatt
     1561 tgtaggaggc ggagtggttc tctccggagc aagcaaagag cttaaggaat ttgtagacaa
     1621 ggtgaatgca cctgtttgcg atacgctcat gggcaagggt gcatatgacg gcacaagtga
     1681 taactacaca ggcatgcttg gcatgcatgg tacaaagacc tcaaaccttg gagtttcaga
     1741 gtgcgatctt ttgattgcga ttggtacacg tttttcggac agagtgcttg gcaatcctaa
     1801 aaagtttgca gaccaggcca agatattaca gtttgacgta gacgcagcag agattaataa
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn acacagcttg accacaattc atgggttgag catgtgcttg catatgcaaa
     1981 gacttttcct cttacatatc acaaagaggg acttagcgga ccatttgtta ttgaaaaaat
     2041 ctatgaaatg acaaaaggaa atgctataat ggttactgag gtcggccagc atcagatgtg
     2101 ggcagcccag tattacaaat acagcaagcc gagaaccctt cttacatcag gaggtcttgg
     2161 taccatggga tacggtctcg gtgctgcaat cggtgcccag acagctaatc ctgacaggac
     2221 tgtagtcaat attgcaggtg acggatgctt cagaatgaac atgaacgagc ttgcaacagc
     2281 ttcaagagaa aagcttcctc ttatcgaggt tataataaac aaccatgtac ttggaatggt
     2341 acgccagtgg cagactcttt tttacgagaa gcattactcg gcaaccgtac tcgatgatgg
     2401 tgtggattat gtaaagcttt cagaagcaat gggagccacc gcacgacgtg ttaagaccca
     2461 ggaagaattt gaaaaagcat ttgcagatgc cttaaagagt aagacaccgt ttgtcatcga
     2521 ttgtatcatt gattcagatg acaaggtatg gcctatggtt gcaccgggca agcctatcaa
     2581 cgagtctttc gatgaagagg actacaatgc gacacaagga ggaaattaga atgagtagag
     2641 tttacaattt ttcagcagga cctgcagttt tacctgagga ggtattgcag gaagcagcag
     2701 cagagatgat ggattataag ggaagcggaa tgtcggttat ggagatgagc cacagatcta
     2761 aatggtttga tgacatcatc aaggatgccg agaaagatct ccgcgagctt atgaatatac
     2821 ctgacaatta caaggtgctt ttccttcaag gtggtgcttc acagttcttc gctgaggtac
     2881 cgatgaacct tatgaaaaat aaaaaagcag gatacattat cacaggtcag tgggctaaaa
     2941 aggcgtttgc cgaggctaag atttacggag acgccgtaga gcttgcttcg tctgcagatg
     3001 agaccttctc atacatacct gactgctcag atttaccaat cacagatgat atggattatg
     3061 tatacatttg cgagaacaat actatctacg gaacaaagta caagaagctt ccaaacacaa
     3121 agggaaagaa ccttgtagct gatgtatctt catgcttctt atcagagcct gtagatgtat
     3181 caaaatatgc tgttatctat ggcggagtac agaagaatgt aggacctgcc ggagtagtta
     3241 tagcaattat cagagaggac cttatcacag atgattgtct accgggcaca ccaaccatgc
     3301 tcaagtggaa gactcaggcc gataatgatt ctctttacaa tacaccacca tgctacggca
     3361 tctatatctg tggcaaggta ttcaaatggc tcaagaagat gggcggtctt tcagttatga
     3421 aggagagaaa tgaggagaag gccaagattc tctacgattt ccttgatcag agcaagctct
     3481 ttaagggaac agtaagaaaa gaggacagat cactcatgaa tgttccgttt gtaacaggag
     3541 atgcagagct tgatgccaag tttgtaaagg aggcaactgc agccggattt gtaaacctta
     3601 agggacaccg tacagttggc ggaatgagag catctatcta caatgctatg ccaaaagagg
     3661 gcgttgagaa gcttgtagag tttatgaaga agtttgagga ggagaatgca taatgttcaa
     3721 ttacacatgc ttaaacccaa tcgcaggagt aggccttgac ctgttctctg acgattataa
     3781 aaaggttgat gatattaagg atgcagatgc agcacttgta agaagtgccg ctatgcatga
     3841 catggagctt ggtgacaagg tacttgctgt ggcaagagca ggagcaggag taaacaatat
     3901 tccgcttgac aagtgtgccg agcagggaat cgttgtattc aacacaccgg gagccaatgc
     3961 aaatggtgtt aaggagcttg tgtttgccgg aatgctttat gcatcaagag atatcgttgg
     4021 aggtatcgac tggtgtcttg caaaccagaa cgatgagaat attgcaaaga cagccgagaa
     4081 gcagaaaaag aactttgcag gaacagagat ttcaggaaag aagctcggtg ttatcggact
     4141 tggagctatt ggtgtacttg ttgcaaatgc tgcagtacat atgggcatgg atgtttacgg
     4201 atatgatcca tacatctcag taaatgccgc atggaacctt tcaagaagcg ttaagcatat
     4261 cagcaatgtt gaggacatct acaagaactg tgatttcatc acaatccatg tacctcttct
     4321 tgattcaaca aagaagatgg taaacgctga tgctattgca atgatgaagc caaccgctat
     4381 cgtattaaat tttgccagag atcttcttgt tgacgaagag gctatggtag atgcacttgc
     4441 caagggaaag gttaagaagt atgtttcaga tttccctaac aacacaactg ttggagcaaa
     4501 gggatgcatc gtaacacctc atcttggagc atctacagca gagtcagagg acaattgtgc
     4561 agttatggct gtcaaggagc tgagagatta cttagagaat ggtaatatcg ttcactcagt
     4621 caacttccct gactgcagca tgggtgcatg cacagcttca ggcagagttg gaatccttca
     4681 caagaatgtt aagggtatga ttggacagat tacaacagct cttgcagagg ctgatatcaa
     4741 tgtatctgat ctgacaaaca agggcaaggg tgactatgca tattcacttc ttgatcttga
     4801 ttcagctatt gatgcttcta ccgttgagaa gctttctgcc attgacggag tgctcagagt
     4861 gagagttatt aaatagtgaa ttaatatttg atttaaatac tgatccccca tattttatat
     4921 gggggattta gtgggttata aggaaataac aaatatannn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnag agatataaga gatatatagg agattatatg atcagacaaa ttcttggtga
     5041 agtatcagtg ccggttgcaa ataccgaggt acagcaggtt attgaaaacc cgggcattgt
     5101 aaagacatat ctggagaagt atatgccatc gcttatcagc tttctcgtgc agcttgttgt
     5161 tgcgatagtg atactgctta taggtattaa ggtgattaaa tcagtcgtaa aaataattaa
     5221 aaaaggattt gataaaagca gaatggacgc tgcagtggca tcatttctag caaatgttat
     5281 taaatacttt ttgtatttta ttcttgtcat ggcactgctt tcgggctttg gagtggcgac
     5341 cggctctgtt atagcggttc ttggctctgc cggacttact gtaggtatgg cattgcaggg
     5401 cagtctgtcg aatttcgcag gaggcgtgct tattcttttg atgaagccgt ttagcgtggg
     5461 cgattacata gttgacggtt caagtggtac agagggcact gtaaaggata taaatctgtt
     5521 ttatacaagg cttttgacaa tagataacaa gatggttatg ataccaaacg gaaagcttgc
     5581 tgattcgtgt atcacaaatg tatctatgat ggataagagg atgcttgatt tgagagtaac
     5641 agtatcctac agcacagatt tagcatttgc aaagagtgtg ctggaatctg ttgtcacatc
     5701 agcagatacg atgcttcacg atgagccgag caatgtgttt gtgagtgagc tgcaggattc
     5761 ggcaatagag cttggtgcga gaatctgggt gaagaacgag gattactgga acacaaaatg
     5821 gcagcttaca gaagaaatta aaacagcatt tgataaaaat aacatcgaaa ttccgtttcc
     5881 acagatggat gttaatattc aaaagaggag catagactct gacttttaaa gccggatgtt
     5941 gcagaatatt actttaacag attttttgtg gtaaatatac ttataattta actataatat
     6001 gtaaaaatgt ggtatactat catcatcgca ttttaggagg aataattgat gacaattcac
     6061 gatttggctg aatacgagat tttagatgag cacagagtgg aggatgtcca gtcggacgga
     6121 ttcattctga gacacaaaaa aagtggtgca aggattgcca ttttatcaaa taatgatgat
     6181 aacaaggtct tttatatagg cttcaggact ccgccggagg atgagacggg tgtaccgcat
     6241 attattgagc ataccacact ctgcggttca aagaagttcc cggtaaagga tcccttcatt
     6301 gagctggcaa agggctcgct caatactttt ttaaatgcca tgacataccc tgacaagaca
     6361 gtttatcctg tcgccagctg caatgatcag gattttaaga atctcatgga tgtgtatctg
     6421 gatgcagtgt ttaatccgaa tatcacaaag tacgaggaga ttttcaagca ggagggctgg
     6481 cactacgagc ttacaggcaa ggatgatgag cttaagataa acggagttgt gtacaatgag
     6541 atgaagggag catactcatc gccggatgag gtgctttcaa gccagattta ccgctcgctt
     6601 ttccctgaca atacctacag caaggattct ggtggtaatc cggagtatat tcctaagct
//
LOCUS       DPUF01000278            3698 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_6625, whole
            genome shotgun sequence.
ACCESSION   DPUF01000278 DPUF01000000
VERSION     DPUF01000278.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_6625"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="DHV81_09430"
     CDS             <1..1005
                     /locus_tag="DHV81_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCI94644.1"
                     /translation="IENAMSNFINHFSLDCALIIDLHAKEPQVLYNDTECDMTDEVIK
                     GICDIMIEYPEGFATSKIGEGFYEHENIISYFGADDVCSFVAVPFIKNDALSSVLIAY
                     VRMKDNWHGSIERYMLNEDDLSIFKLLFRELEYSIARIEANEKANEMNRRLKQAAVTD
                     MLTGIYNRAGMYQEIEKLEKRISVTSGGMDVGLMFIDLDNFKHYNDTYGHDVGDLILK
                     EMAFIFREVAKDRGFVSRYGGDEFIIVIESCARYELENIAKDIYARIADADGFSRQIK
                     KYLNHEVEFNEEKNITCSIGISYERNVTSESQITELIKKADDLMYTVKTGEKGHYAFF
                     "
     gene            992..2218
                     /locus_tag="DHV81_09435"
     CDS             992..2218
                     /locus_tag="DHV81_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI94645.1"
                     /translation="MHSFKSDEFKIRVLTKDDAEEYNALLRYAFQVTEADLAETGWKD
                     DEIKQSKFPVLERADVLGCFNIIDKKEELVAQFAVYPLDMNIYGERYEVGFVTSVCTY
                     PEYTGHGLMKKLMRKSLTRMRDSNKSFALLYPYSIPLYRGLGWEIISNKMTYVIKDRQ
                     IPTKLKEPGYVRRVQWDDEQFKKLHTHFAAKTHGCLYRNNLAWEEYFRWDEDDTMVAI
                     YYNEDDEPCGYMVYLISSDVMHIKELIYLNREAQLGLWEYIHAHDSMIDEVRGNNYYS
                     EPIAFELDDSDIKETIRPYAMGRIIDVSQFISQYNCDPDGPGGCFSFEIADELLPWNN
                     GTFVIDFEKGHCALTDKKPQYHLSMSIGTFTTLLLGYKSAEKLLQMGKIQTTYDAVAK
                     LDDILYHEIPYVSDYI"
     gene            2218..3138
                     /locus_tag="DHV81_09440"
     CDS             2218..3138
                     /locus_tag="DHV81_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCI94646.1"
                     /translation="MIKGQKVKENNMSNYVISCCSTADLTREHMLERNIRYACFHYEL
                     DGKEYMDDLGQSMPLPDFYKAMEAGAMTKTSQINQEEYMEYFRQFLDEGYDVLHCTLS
                     SGISGTVNSARLAKEALEEEYPDRKIYVIDSLAASSGFGLLMDKLADLRDSGMDIDTL
                     ADWTMEHRLEVNHWFFSSDLTFFIRGGRISKTAGFVGNVLNICPFMNVSNEGKLIVRE
                     KIRTKKKAIKEMLVKMQALCKDGADYSDKVYISNSACHEDAKAVADLIEAAFPKMKGK
                     VEINDIGTTIGSHTGPGTVALFFWGNKRED"
     gene            3307..>3698
                     /locus_tag="DHV81_09445"
     CDS             3307..>3698
                     /locus_tag="DHV81_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCI94647.1"
                     /translation="MKGYEKYERSYFLPPECTYDWVKKDYIDKAPLWCSVDLRDGNQA
                     LIEPMSLEEKLDFFKMLVDIGFKEIEVGFPAASETEFNFTRALIERDMIPDDVTIHVL
                     TQAREHIIKKTFKALEGAPRAVVHLYNST"
BASE COUNT         1117 a          654 c          943 g          984 t
ORIGIN      
        1 attgaaaatg ccatgagcaa tttcataaat catttcagtc tggactgtgc gctgattata
       61 gatttgcacg ctaaggagcc gcaggtactt tataatgata cagaatgtga tatgacagat
      121 gaggttataa agggcatatg tgatatcatg atagaatatc cggagggttt tgccacatca
      181 aagattggag aaggcttcta cgagcatgaa aatatcatct cgtacttcgg cgcggatgat
      241 gtatgttcat ttgtagcggt gccttttatc aagaatgatg cgctttcaag tgttctcata
      301 gcatatgtgc gcatgaagga taactggcat ggctccatag agcgatacat gctcaacgag
      361 gatgatttga gcattttcaa gctattattc agagagcttg agtattccat tgcccgtatt
      421 gaggccaatg agaaggcaaa tgagatgaac aggagactca agcaggccgc agttaccgat
      481 atgctgacag gcatatacaa cagagcaggc atgtatcagg agattgaaaa gcttgagaag
      541 cgtataagtg tgacatccgg tggtatggat gtggggctta tgtttattga cctggacaac
      601 ttcaagcatt ataatgatac gtatggtcat gatgtaggag atctgatact taaggagatg
      661 gcctttatat tcagggaagt ggcaaaggac agaggctttg tgagcagata tggcggtgat
      721 gagttcatca tagtcattga gtcatgtgcc agatacgagc ttgagaatat tgcaaaggat
      781 atctatgcga ggattgcaga tgcagatggc ttcagcaggc agataaagaa atacctgaat
      841 catgaagtag agtttaatga ggagaagaat atcacctgtt ccattggtat ttcatatgag
      901 agaaatgtta ccagtgagag ccagattact gagctcatta agaaggcgga tgatctgatg
      961 tatacagtaa agacaggtga gaaaggacac tatgcattct tttaaatcag atgaatttaa
     1021 gataagagta ctgacaaagg acgatgcgga ggaatacaat gcactgcttc gttatgcttt
     1081 tcaggtaaca gaggcagatc ttgccgagac cggttggaag gatgatgaaa tcaagcagtc
     1141 gaagtttccg gtgcttgaga gagctgatgt gcttggctgc ttcaatatca tagacaaaaa
     1201 agaggagctt gtggctcagt ttgctgtata tccgctcgat atgaatatat atggtgagcg
     1261 ctacgaggta ggctttgtca caagtgtgtg cacgtatcct gagtacacag gccatggtct
     1321 catgaaaaag cttatgagaa agagtcttac gaggatgcgc gatagcaaca agtcgtttgc
     1381 ccttttgtat ccatactcca tacctcttta cagagggctt ggctgggaga ttatctcaaa
     1441 caagatgaca tatgtgataa aggacagaca gattcctaca aagcttaagg agccgggcta
     1501 tgtgcgccgt gtgcagtggg atgacgagca atttaagaag cttcatactc actttgccgc
     1561 gaagactcac ggctgtctgt acagaaacaa tctggcttgg gaggagtact tccgctggga
     1621 tgaggacgat acgatggtcg ccatctacta taacgaggat gatgaaccgt gtggctatat
     1681 ggtgtatctt atcagctccg atgtcatgca catcaaggaa ctcatctact taaaccgtga
     1741 ggcgcagctt ggcctgtggg agtacattca tgcgcatgat tccatgattg atgaggtcag
     1801 aggcaacaac tattacagcg agcccatcgc ttttgagctc gatgacagtg atataaagga
     1861 gacaataaga ccatacgcca tgggcagaat catcgacgtg tcgcagttta tatcacagta
     1921 taactgtgac ccggatggac cgggcggctg ctttagcttt gagatagcag atgagcttct
     1981 gccgtggaac aatggcacat ttgtcattga ttttgagaaa gggcactgtg ctctcacaga
     2041 caaaaagcca cagtatcatc taagtatgtc tattggcacg tttaccacac tgcttttagg
     2101 ctacaagagt gctgagaagc ttttgcagat gggcaaaata cagactacgt atgatgctgt
     2161 tgcaaagctt gacgatattt tatatcatga gataccatat gtatctgact acatataatg
     2221 ataaaaggtc aaaaggtaaa ggaaaataat atgtcaaatt atgtgattag ctgctgttca
     2281 acagcagatc ttaccaggga gcatatgctc gagagaaata ttaggtacgc atgctttcac
     2341 tatgagcttg atggtaaaga gtatatggat gatttaggac agtccatgcc actgccggat
     2401 ttctacaagg caatggaggc aggtgccatg acaaagacct cgcagatcaa tcaggaggag
     2461 tacatggagt acttcaggca gtttctggat gagggatatg atgtgcttca ctgtacacta
     2521 agctccggta tatcaggtac agtcaactcg gcaaggcttg caaaggaggc tctcgaggag
     2581 gaataccctg acagaaagat atatgtgatt gattcgctgg cagcatcgtc aggctttggt
     2641 cttttgatgg ataagctcgc tgacctcagg gatagcggca tggatattga tacactggct
     2701 gattggacca tggagcacag acttgaggtg aatcactggt tcttctcatc agaccttaca
     2761 ttttttatca ggggcggccg tatctcaaag accgcgggat ttgtgggaaa tgtgcttaat
     2821 atctgtcctt tcatgaatgt gagcaatgag ggaaagctta ttgtgcgtga gaaaatccgc
     2881 actaaaaaga aggcaataaa ggagatgctt gtgaagatgc aggcgctctg caaggacggt
     2941 gccgattatt cagataaggt ctatatatcg aattccgcat gccatgagga tgctaaagcc
     3001 gtagctgatc ttatagaggc tgcattcccg aagatgaagg gcaaagtaga gataaatgat
     3061 atcggtacga ctataggaag ccacaccggt ccgggaacgg ttgcattgtt tttctggggc
     3121 aataagagag aggattagcc ggatatttca gcctaaatat ctgattagaa taatcaaaat
     3181 caatacagtt gattatattt ttataaaaaa ttaaattaca tcaataaaag tgattaaata
     3241 taatcattcc aataactgcg cttatgtgtt acaattatat cagttcaaac gaaaagaggt
     3301 tttaagatga aaggatatga aaaatacgag cgcagttatt ttctgcctcc tgaatgtact
     3361 tatgactggg ttaagaagga ttatatagat aaagcaccac tgtggtgttc ggttgattta
     3421 cgtgatggca accaggctct gatcgagccg atgagtcttg aggagaagct tgacttcttt
     3481 aagatgcttg tagatatagg ttttaaggaa atcgaggtag gtttcccggc agcttctgag
     3541 acagagttca atttcacgag agctcttatt gagagggata tgattcctga tgatgtcact
     3601 atacatgtgc tgacacaggc cagagagcat atcatcaaga agaccttcaa ggcactcgag
     3661 ggagcaccga gagcagttgt acatctgtac aattccac
//
LOCUS       DPUF01000279            3170 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_5849, whole
            genome shotgun sequence.
ACCESSION   DPUF01000279 DPUF01000000
VERSION     DPUF01000279.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_5849"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            76..1455
                     /gene="murC"
                     /locus_tag="DHV81_09450"
     CDS             76..1455
                     /gene="murC"
                     /locus_tag="DHV81_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCI94648.1"
                     /translation="MYKIDFSKPIHIHFIGIGGISMSGLAEILHEEGFTISGSDSKES
                     DLTKTLAAKGIKVIYGQSADNITSGIDLVVYTAAIHESNPEFAAAKAAGIPMLTRAEL
                     LGQIMDNYDYSIAVAGTHGKTTTTSMISEILLAAQTDPTISVGGILSSIHGNLRVGDS
                     EYFISEACEYTNSFLNFRPRYSIILNIEAEHLDFFKDINDIRHSFREYASNTLAGGAT
                     IINGEIDRYEEIVAGLPQKIITYGFDSKYDFYPENITYDDKACASFTAMRQGSPLFDV
                     KLSVPGAHNVSNALAAIALSTTLGLDTESILTGLDKFGGADRRFQYKGKVNGVTIIDD
                     YAHHPTEIRATLTAAQKYPHDRLVLCFQPHTYSRTKAFLNDFADVLSMADVVVLADIY
                     AAREQNTYGISSKDILDLLLEKGVNAHYFPSFEEIEKFLSENCVNGDLLITMGAGNVV
                     EIGEDLLKK"
     gene            1713..2954
                     /locus_tag="DHV81_09455"
     CDS             1713..2954
                     /locus_tag="DHV81_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HCI94649.1"
                     /translation="MTAINISSDIATSFTTVSDIFIDQYMPKANGEFVKVYLYLLRAT
                     GSGAGIATISEIADHFSNTEADIIRALNYWASEGILQVQTGADGQIIGINLCSLSVSG
                     MQAAQSNIQSAVADNAAQNNLQNGVVNNATQNNLQNSVVNNAAQNISTVNTRMHDSVV
                     EKLKSQTPDKAASSQKEYTLDEIKEFRKNPNISELFFIIETYLKHTLSSTDTNMVLYW
                     LDELHFSTDLVEYLVEYCITKGHSSLRYMNKVALGWADAGIKTVDQAKDDAAAHSQIY
                     YSVMKALGITGRNLVDSEVSLINKWVGEYGFDIELVKAACSKTISAIQKPSFEYTDSI
                     LANWRKKDVHTLKDVEVLDANFAKANKVSATGSSQGTNAANGSSKPKSNNSSSKNKFN
                     NFNQRNNDYDKLEKLFLNSTV"
     gene            2992..>3170
                     /locus_tag="DHV81_09460"
     CDS             2992..>3170
                     /locus_tag="DHV81_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCI94650.1"
                     /translation="MALTNAQYDEIMRGYDKRQLQNKYIHDKRIEEAYRKAPRLREID
                     SEIASASVRQAYRLM"
BASE COUNT          939 a          712 c          669 g          850 t
ORIGIN      
        1 acgcatcggc tttttgttct cagtgtttct taatagttac aatataaatt ataataaatc
       61 aggcaggtaa aattaatgta taaaatagat ttcagtaaac caatacacat ccatttcata
      121 ggcatcggcg gtatcagcat gagcggtctc gcagagattc tgcatgagga gggctttaca
      181 atttccggct ccgattcaaa ggaatcagac ctcacaaaaa ctcttgctgc caagggcata
      241 aaagtcatat atggccagag cgcagacaat atcacatccg gtatcgacct ggtggtttac
      301 actgctgcta tccacgaaag caatcctgag tttgccgcag caaaggcagc cggtattcca
      361 atgcttacca gagccgagct tctcggacag attatggaca attacgatta ttccatcgct
      421 gtagccggca cacacggcaa gactaccacc acatccatga tcagcgagat tcttctcgcc
      481 gcccagacag atcctaccat ttccgtaggt ggtattttgt catctatcca cggcaatctg
      541 cgtgtgggcg attcagagta cttcatctct gaggcatgtg agtacacaaa cagctttttg
      601 aatttcagac cacgctacag tattatttta aatattgagg ctgagcatct ggatttcttc
      661 aaggatatca acgacatcag gcattccttc cgcgagtatg catcaaacac tctcgcaggc
      721 ggtgctacca tcatcaatgg tgagattgac cgctatgagg agattgtcgc agggcttcct
      781 cagaagatta tcacctatgg ctttgattca aaatatgatt tctatccgga aaatataacc
      841 tatgacgata aagcttgtgc atcttttaca gctatgagac agggtagtcc acttttcgac
      901 gtaaaactct ctgttccggg cgctcacaat gtaagcaatg cgctcgcagc catagcttta
      961 tccaccacac tcggtctcga tacggagtct atcctcacag gccttgacaa atttggcggt
     1021 gctgacagac gtttccagta caagggcaag gtaaacggcg tcacaatcat tgatgactac
     1081 gctcaccatc caaccgagat ccgcgcaaca ctgacagctg cacaaaaata tccacatgac
     1141 aggcttgtcc tttgcttcca gccacacacc tattccagaa ccaaggcatt cttaaatgat
     1201 tttgccgatg ttctttcaat ggcagatgtt gtggtgcttg ctgatatcta tgctgcgcgt
     1261 gagcagaaca catatggtat ctcatctaag gatatcctgg atttattgct cgaaaaaggt
     1321 gtgaatgcgc actatttccc atcatttgag gaaatcgaga aatttttatc agaaaattgt
     1381 gtaaacggtg acctgttgat aacaatgggt gccggaaatg tagtagaaat cggcgaagac
     1441 ctgcttaaga aataagttat ccacgttatc cacatacaaa atgttaacaa ctctcatttt
     1501 gtgtgtggat tttttaaata attttcaaca gcttatatac cctgtgatac attgattttt
     1561 caattatttt acacagttat ccacgttatc cacatttttt tgtggactcg gaaagcccga
     1621 gtttacaagg cttgtagcac ttttaccatc ttcacacggt taaaatattg tgctataatt
     1681 gctcacaggt taagcgaaag agagtttttt atatgacagc gataaacatt agcagtgaca
     1741 tagcaacatc attcactact gtgtcagata tttttataga tcaatacatg ccaaaggcaa
     1801 acggtgagtt tgtcaaggta tacctttatc tgctccgtgc gacaggctcc ggggcaggca
     1861 tagcaactat ttcagagatt gccgatcatt tcagcaatac tgaagccgac atcatacgtg
     1921 ccctcaatta ctgggcctct gagggcattt tgcaggtgca aaccggcgct gatggtcaga
     1981 ttattggaat caacctctgc agtctttctg tatccggtat gcaggctgct cagagtaata
     2041 tacaaagcgc tgtggctgat aatgccgcgc agaataattt gcaaaacggt gttgttaata
     2101 atgctacgca gaataatttg caaaacagcg tggttaataa tgctgcacag aatatttcta
     2161 ccgtgaatac tcggatgcat gattctgttg tggaaaagct taagagccag actcctgaca
     2221 aagctgcttc ttcacagaag gaatacacac ttgatgagat taaggagttt agaaagaatc
     2281 ctaacatttc agagcttttc tttattatag agacatatct gaagcatacg ctctcatcca
     2341 cagacaccaa tatggtgctc tactggctgg acgagcttca tttttctacc gacctggtag
     2401 aatatctcgt agagtactgc attaccaaag gacattcaag ccttcgttac atgaacaagg
     2461 tcgctctcgg ctgggccgat gccggcataa agaccgtaga ccaggcaaag gacgatgcag
     2521 ccgctcactc acagatttac tattctgtaa tgaaggctct aggcatcaca ggccgcaatc
     2581 tggttgactc tgaggtgtca ctaataaaca aatgggtcgg cgaatacggt ttcgatatag
     2641 agcttgtaaa ggccgcctgc agtaaaacca tttcagctat ccagaagcca agcttcgagt
     2701 acacagactc gatactcgcg aactggagga aaaaggatgt acatacatta aaggatgttg
     2761 aagtgcttga tgccaacttt gccaaggcca ataaggtctc tgctacaggc tcatcacaag
     2821 gcacaaatgc tgcaaacggt tcctcaaaac caaaaagcaa taattcaagt tccaagaata
     2881 aatttaacaa cttcaatcag cgcaacaatg attacgacaa gctggagaag ctgtttctga
     2941 attctacggt atgatttttc aagttagatt tacacatccc aaggaggata tatggcactt
     3001 accaatgctc aatatgatga gataatgcga ggctacgata aacgccagct tcagaacaaa
     3061 tacatacatg acaagcgtat tgaggaagcc tatcgcaagg cgccccgcct cagggagatt
     3121 gactcagaga ttgcatccgc atctgtcagg caggcgtaca ggcttatgga
//
LOCUS       DPUF01000280            9108 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_16003, whole
            genome shotgun sequence.
ACCESSION   DPUF01000280 DPUF01000000
VERSION     DPUF01000280.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_16003"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            <1..>254
                     /locus_tag="DHV81_09465"
     CDS             <1..>254
                     /locus_tag="DHV81_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCI94651.1"
                     /translation="EVMFESAKFARDNIRKSSRALGQSSDSSALYSKGVNEYTTAMAM
                     KRALHLMEELGCGKVSSTHVEVTTGNSLEPKEMKVSIAKV"
     assembly_gap    255..426
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <427..1371
                     /locus_tag="DHV81_09470"
     CDS             <427..1371
                     /locus_tag="DHV81_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCI94652.1"
                     /translation="AFMPTAQVTLGGLNLRQQTERKIKEVLCAAGAYEGIHYSFFSPA
                     DLDLLRFPEDAPERHAIRLINPINVDLSLMRTTLASEMLYAIARNQKKGILEGRIFEL
                     GNIFIAKELPLTEYPDERETLCAGVFGEDESFFTIKGLAETVAYALDVKFTYKPAQKP
                     FLHPYQTAEVFCDGQKIGYLGQVRYEICDELDMRVPAYVMELDLRVLSQWYGKDRVFT
                     PISKFDDEKRDFAFVVDKDITCEQIENGIREACDYVSDVTLFDVYEGVQLPPNKKSMA
                     YSVVFTPKDEELKTKKVEGFVKDILAHLKETAGITLRG"
     gene            1403..>2448
                     /locus_tag="DHV81_09475"
     CDS             1403..>2448
                     /locus_tag="DHV81_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCI94653.1"
                     /translation="MDVKDFDYDLPEELIAQDPLEDRSSSRLMVLDKKTGEVSHHVFK
                     EIVKYLRPGDCLVLNNTKVIPARLFGVKEGTMAKIEILLLKRRQNDVWETLVKPGKKA
                     KAGTKIIFGDGLLTGEVIDVVDDGNRLIQFSYEGIFEEILDKLGQMPLPPYITHQLKD
                     KNRYQTVYAKYDGSAAAPTAGLHFTKELLQQVKDKGVDIAEVTLHVGLGTFRPVKVDN
                     VLDHHMHSEFYMVSQEAADKINNAKKNGGRIISVGTTSTRTLEAASDENGMLKECSGW
                     TDIFIYPGYSFKVIDCLITNFHLPQSTLVMLVSALAGREHVLNAYKEAVEERYRFFSF
                     GDAMFITNDMHMDD"
     assembly_gap    2449..2519
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2568..2909
                     /locus_tag="DHV81_09480"
     CDS             2568..2909
                     /locus_tag="DHV81_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HCI94654.1"
                     /translation="MEEKRRHKRLDIDVSVQLERLDEDGVTTLKYCHVDVTDISRSGI
                     GFNAKVPLDIHTYYDTKIQIWTKEVVDAVVEIVRRTDSEEGVYHYGAVFIGMTDTDAL
                     KIDIYQIFNDL"
     gene            2899..4182
                     /gene="aroA"
                     /locus_tag="DHV81_09485"
     CDS             2899..4182
                     /gene="aroA"
                     /locus_tag="DHV81_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCI94655.1"
                     /translation="MIYKVKKVNHPHDIVTSVPGSKSITNRALLIAALASGRSVLKGC
                     LFSDDSRHFIDALIRLGFPVLVDEDKRKITITGFGGRIPKNEAEIDVGSAGTAARFLT
                     ALLGLSKGRYHIVSSEQMKKRPMKDLLVSLEKLGAHIEYDENEYHFPFTIGNTGEYAD
                     TVDINVDKSSQFLSALLISAIVMEKNFTINVTGTHGMAYVEMTRLMMKQFGLDVMQDK
                     KNNSFIIPKKAAYESLDYDIEPDVSAACYFYAMSPLLHVKSKVMGVHQNSLQGDVAFL
                     DVLADMGCTISDEADGIVCMPPKNAHIHGGSWDLSTFSDQALTLAAIAPFANAPVGIE
                     GISHIRLQECDRINAIEENLTELGVRVEEIENGLKIYPAERIKPCKIKTYDDHRVAMS
                     FTLPGLKAEGVEIIDPYCCRKTFENFYEVLEESVY"
     gene            complement(4313..5410)
                     /locus_tag="DHV81_09490"
     CDS             complement(4313..5410)
                     /locus_tag="DHV81_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HCI94656.1"
                     /translation="MHFDKVGFIGLGLIGGSIARKMKENNPKVSIIATAGHQQTIDDA
                     FGLGLIDNNELLELNSFKDCDVIFLCAPVNKNIEYLTQLKDIIKDDCIITDVGSTKTQ
                     IHEKVIELGLEHNFIGGHPMTGSEKTGILNSDKQLLENAYYIITPTAATSEENQNDFR
                     QFVLSLGSIALILDYREHDHATAAISHLPHMIAYSLVNLIEHIDSEKETMKTIAAGGF
                     RDVTRIAASSPVMWENICESNKTQLLSLMDQYEANFHKLREIIAEGSSQKMIDYFQDS
                     KNYRDSLTLPGAKIRKDFHEVFVDIADEAGGIAVLASLLAFNGISIKNIGIINNREFE
                     EGVLRIEFYDEDSLESAISVLKERNYTIHRR"
     gene            5667..7757
                     /gene="fusA"
                     /locus_tag="DHV81_09495"
     CDS             5667..7757
                     /gene="fusA"
                     /locus_tag="DHV81_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516578.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCI94657.1"
                     /translation="MNVYTTEKIRNVVLLGHSGCGKTSLVEAMAYLAGQTTRMGTVAD
                     GSTISDFDKEEIKRQFSIHTSVVPIEWDNVKINILDTPGFFDFVGEVEEAVSAADAAI
                     IVVSGKAGIQTGTKRAWEICEKYKLPRMIFVTDMDIDNASFKDVVLKLQELYGKKIAP
                     FHLPIRENEKFVSYVNVIQQRAKRWNESGGVDKFDVPEYSKENLGICREALVEAVAET
                     SEEFMDRYFGGEEFSDDEIRAALRANVFEGSIVPVLMGSNILARGMYTLMADIVKYLP
                     SPEKRSCTGINTKTNEVYNADYDFAKSKSAYVFKSIVDPYIGKYSLIKVNSGVLKTDD
                     VLYNYHRDCDEKIGKLYVMRGSKVEEVKELHAGDIGALAKLTKTLTTDSLSTRNMPVT
                     YLRTSISKPYVAMRYKAKKKGDEDKISQSLQKLLMEDLTLNSVNDSENGQTVLYGIGD
                     MQLEVVASELLEKYKVEIELMRPKVAFKETIRKKSDVEYKYKKQSGGHGQYGHVKMTF
                     EPSGDMDTPYVFEQTVVGGAVPKNFFPAVEKGIAESVKKGPLAAYPVTGIKAVLYDGS
                     YHPVDSSEMAFKVATQQAFKKGIMEAKPVLLEPIATLKVVVPEGYTGDVMGELNKRRA
                     RVMGMNPTDNGYQEIVADIPYLELYGYNATLRSMTSGSGTFSYEFARYEQAPDDVAAK
                     QIEERASKLVEVEE"
     gene            7741..9042
                     /locus_tag="DHV81_09500"
     CDS             7741..9042
                     /locus_tag="DHV81_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94658.1"
                     /translation="MKLKSRIALIILLTLIFTNTHVFASEIKEAEAASFASGPSLNTL
                     VDNMSESDGVSYIYYNLGGAANNINNYGYITPKQKFMGLREPHKYGYKFDGWYLDEHF
                     SKKADVLTYEKANGYVVYAKWVRTINNEYSVEHYNYRSNKKAHTLALKDCDYDFIDEI
                     DIPGMPETKENDFLNNYIFSEAQCPQGICITDEYVLITSYSDDKGSLGELMVFDREDG
                     EYLVTLGMDANSHLGGIAFDGENVWVCNSYDTTVERISYDFISLMATANSKQVIDATG
                     VVDVFDVGNKPSCITFYGGRLWIATHNILFKSKMVAYYYDKKDDRLTSLSTYTIPARV
                     QGVTFDASGKVYLSTSYGRNESSYIKCYKSLIALSSRPNSPDITIEMPPGSEELDSVD
                     KRLYVIFESAGEKYLEGTDGKGNSPAPIDKILRINTDSFKN"
BASE COUNT         2669 a         1583 c         2222 g         2391 t
ORIGIN      
        1 gaggtgatgt ttgagtctgc aaaatttgca agagacaata tcagaaagag ctcacgtgcg
       61 ttgggacagt catctgattc gagtgcactt tactcaaagg gtgtaaacga gtacacaaca
      121 gccatggcta tgaagagagc acttcacctg atggaggagc ttggctgtgg taaggtttct
      181 tcaactcacg ttgaggtgac tacaggaaat tcgcttgagc caaaagagat gaaggtttct
      241 attgcaaagg taaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnngcat tcatgcctac agcacaggtt acactcggag gactcaacct tagacagcag
      481 acagagcgca agataaaaga ggtgctctgt gcagcaggcg cgtatgaggg aatacattac
      541 tcattcttct cacctgctga tcttgatttg ctcagattcc cggaggatgc acctgaaaga
      601 catgctatca ggcttataaa cccaatcaat gttgacctct cgcttatgag aacaacactt
      661 gcttctgaga tgctttatgc catagcaaga aaccagaaga agggtatcct ggagggaaga
      721 atctttgagc tgggaaatat ctttatcgca aaggagcttc cgcttacaga gtatccggat
      781 gagagagaga cactttgcgc aggcgtattc ggtgaggatg aatcattctt tactataaag
      841 ggactggctg agactgtagc atatgcgctt gatgtgaagt ttacatacaa gccggctcaa
      901 aagccattcc ttcatccata tcagaccgca gaggtattct gtgacggaca aaaaatcgga
      961 tatctcggtc aggtaagata tgagatatgc gatgagcttg atatgagagt accggcatat
     1021 gtaatggagc ttgacttaag agttctttca cagtggtatg gaaaggacag agtatttact
     1081 ccaatttcaa aatttgatga tgagaagaga gattttgctt ttgttgtgga caaggatatc
     1141 acatgtgagc agattgaaaa cggtatcagg gaagcctgtg attatgtctc agatgttaca
     1201 ctgtttgatg tttacgaggg tgtacagctt cctccgaata agaagagcat ggcgtacagt
     1261 gtagtattta caccaaagga tgaggagctt aagactaaga aggtcgaggg ctttgtaaag
     1321 gatatacttg ctcatcttaa agagactgca ggtatcacac ttcgtggtta gacgttagta
     1381 tagtgttagg aataggaaaa ttatggacgt aaaagatttt gattatgatc ttccggagga
     1441 gctaatagca caggatcctc tggaggaccg ctccagctca agactcatgg tgcttgacaa
     1501 gaagacagga gaagtcagcc atcatgtgtt caaggagatt gttaaatatt taagaccggg
     1561 tgactgcctt gtgctcaata atacaaaggt cattcctgca cgtctgtttg gcgtgaagga
     1621 aggaactatg gcaaagatag agatacttct tctaaagcgc agacaaaatg acgtatggga
     1681 gactcttgta aagccgggta aaaaggcaaa ggcgggcacg aaaattatat tcggtgacgg
     1741 acttttaacc ggtgaagtaa tagatgtggt tgatgatgga aacaggctga ttcagtttag
     1801 ctatgagggt atttttgagg agatacttga taagctcgga cagatgccat tgccgccata
     1861 catcacacat cagctaaagg acaagaaccg ctaccagaca gtatatgcaa agtatgacgg
     1921 ctcggccgct gcgcctacgg caggactgca tttcacaaag gagcttttac agcaggtaaa
     1981 agataagggg gtagatattg cagaggtgac tttacatgtg gggcttggta cgtttagacc
     2041 ggtaaaggtg gataatgtgc ttgatcacca tatgcattct gagttttaca tggtgtcaca
     2101 ggaggctgca gacaagatta ataatgcaaa gaaaaatggc ggcagaataa tatctgtcgg
     2161 cactacaagc actcgtacct tagaggcagc atccgatgaa aacggcatgc taaaagagtg
     2221 cagcggatgg acggatatct ttatttatcc gggatacagc tttaaggtga ttgactgtct
     2281 gattacaaat ttccatctgc cgcagtctac acttgtcatg cttgtgtcag cattagcagg
     2341 cagagagcat gtcctcaatg catacaaaga ggctgtagag gaaagataca ggtttttcag
     2401 ctttggtgat gcgatgttta taacgaacga tatgcatatg gacgatgann nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     2521 ggtaatttgt tcccttagtc aataatataa tagatggggt aaagtgaatg gaagaaaaga
     2581 gaagacataa aagacttgac attgatgtat cggtacagtt agagcggctg gatgaggacg
     2641 gagtgacaac acttaagtat tgtcatgtgg atgtcacaga tatttccaga agcggtatag
     2701 gatttaatgc aaaggtacct cttgatatac atacatatta tgacaccaag atccaaattt
     2761 ggaccaagga ggttgtggat gcggttgtcg agattgtacg aagaacagac agtgaagaag
     2821 gagtatatca ctatggtgct gtgtttattg gtatgacaga cactgatgca ctcaagattg
     2881 atatttatca gattttcaat gatttataaa gtaaaaaaag taaatcatcc acatgatata
     2941 gtaaccagtg tacctggctc aaagagtatc acaaacaggg cccttttaat cgctgcactc
     3001 gcaagcgggc gcagcgtttt aaaagggtgt ctttttagtg atgattcgag acattttata
     3061 gatgcactga ttcgtctggg atttccggtt ttggtggatg aggataaaag aaaaataaca
     3121 ataacaggct ttggaggcag gataccaaag aatgaagctg agatagatgt gggaagtgca
     3181 ggtaccgctg cacgttttct tacagctctt cttggactgt caaagggcag ataccatata
     3241 gtgtcctctg agcagatgaa gaaacgtcct atgaaggatc tgcttgtatc gcttgaaaag
     3301 ctgggtgccc atattgagta tgatgagaac gaatatcatt ttccttttac catcggaaat
     3361 acaggggagt atgctgatac tgttgatata aacgtagaca agagcagcca gtttttgagt
     3421 gcccttctta tctcggctat agtgatggaa aagaacttta ctattaatgt cacaggaacg
     3481 catggtatgg catatgttga gatgacgcgt cttatgatga agcagtttgg gctcgatgtg
     3541 atgcaggaca aaaagaataa cagctttatt atccctaaga aagcggcgta tgaaagctta
     3601 gactatgata tagagccgga tgtgtcggct gcatgctatt tttatgcaat gagcccgctt
     3661 ttacatgtta agtcaaaggt gatgggggtg caccaaaaca gcttgcaggg agatgtggca
     3721 tttcttgatg tgcttgcgga tatgggctgc acaatttccg acgaggcaga cggcatagtg
     3781 tgtatgccac caaagaatgc tcatatacat ggcggaagct gggatttgtc aacattctca
     3841 gaccaggcgc tgacacttgc tgcgattgca ccgtttgcaa atgcacctgt agggatagaa
     3901 ggcatttcgc atattcgtct gcaggagtgt gacaggataa acgccatcga ggaaaacctg
     3961 acggaattag gtgtgagggt tgaagaaatt gaaaatggct taaagattta tccggcagag
     4021 cgtattaagc cttgtaaaat taaaacgtat gacgatcatc gcgtagccat gtcatttact
     4081 ttgccggggc ttaaggcaga gggtgttgag ataatagacc cttattgctg ccgcaagacc
     4141 tttgagaatt tctatgaggt acttgaggag agcgtatatt aataaagtgt tattggtttt
     4201 ttgcttattt gtaaacagtg ctgcattgga cggtataaaa agagccgtaa aacgaatcaa
     4261 attcgtttta cggctctttg tttttgggat taaaaccata taacaggata aatcatctgc
     4321 ggtgaatagt atagtttctt tctttaagta cgctgatagc cgattcaaga gaatcttcgt
     4381 catagaactc tatgcgcagt acaccctcct caaactcacg gttgtttatg atgccgatat
     4441 tcttgatgct gatgccgttg aaggctaaaa ggcttgcaag cactgcaata ccgcctgcct
     4501 cgtcggcaat atcgacaaat acctcgtgga aatccttgcg gattttggca ccgggaagtg
     4561 tgagtgaatc cctgtaattc tttgaatcct ggaaataatc aatcatcttt tgagaactgc
     4621 cctctgcaat tatttcgcga agcttatgga aattggcttc atactggtcc atgagtgaga
     4681 gcagctgtgt tttgttggac tcacatatgt tttcccacat gacaggggag cttgcagcga
     4741 tacgtgtcac atcgcggaag ccaccggcag cgatagtttt catggtctct ttttcggaat
     4801 caatatgctc aattagatta acaagagagt atgcaatcat atgtggcagg tggctgatgg
     4861 ctgcggtagc atgatcgtgc tctctgtagt ctaaaatgag agctatcgag ccaagtgaaa
     4921 gtacaaactg tctgaaatca ttctgatttt cttcactagt tgctgcagta ggagtgataa
     4981 tatagtaggc gttctccaaa agctgcttgt ctgaattgag tatgcctgtt ttttcagagc
     5041 cggtcatagg atgcccgccg ataaaattgt gctcaaggcc aagctctata accttttcgt
     5101 gaatctgggt tttggtgctg cctacatctg tgatgataca gtcatccttt atgatatcct
     5161 taagttgtgt gagatattcg atatttttgt ttaccggtgc acacagaaag attacgtcgc
     5221 agtctttaaa agagttaagc tctaaaagct cattattatc tatcaggcca agcccaaagg
     5281 catcatctat ggtctgttga tgcccggctg ttgcgataat tgagaccttt ggattatttt
     5341 ctttcatttt tctggctatc gagccgccaa tcagaccgag ccctataaag ccaactttat
     5401 caaaatgcat ttatgtgtat ctcctaattc tttattgtaa ctattcagaa tcaccacacg
     5461 cacattctca aaatcgcact atagtggttt tgaatattta caatatatta tatccaattt
     5521 aacactataa agcaataaat tattggcata tttattccaa tataacaata aaaaaataat
     5581 cgtcagattg cataaaagtg ttgtaaatta tataaaatat tagtacaatg tttacatact
     5641 ataataatgt cagggaggta cttgaaatga atgtttacac tactgagaag attcgaaacg
     5701 tcgtgctttt aggccatagc ggctgtggca agacatcact cgtggaagct atggcatatc
     5761 tggcaggaca gactaccaga atgggcactg tagcggatgg cagcactatc agcgattttg
     5821 acaaagaaga gataaagcgt cagttctcta tacatacatc ggttgtaccg atagagtggg
     5881 ataatgtcaa aataaatatt ctcgatactc ctggtttttt tgattttgtg ggagaggttg
     5941 aagaagctgt atcggctgca gatgctgcaa tcatcgtggt ttcaggcaag gcaggcatac
     6001 agacaggcac aaaaagagca tgggagatat gcgagaaata caagctgccg aggatgattt
     6061 tcgtgacgga catggatatc gacaatgcaa gcttcaagga tgtggtgcta aagctgcagg
     6121 agctttacgg caagaaaata gctccattcc atctgccaat cagagagaat gagaagtttg
     6181 tgagctatgt caatgtcatc cagcagaggg caaagagatg gaatgaaagc ggaggcgtgg
     6241 ataagtttga tgtgcctgag tactcaaagg agaacctggg aatatgtcgt gaggccctag
     6301 ttgaagctgt agctgagaca agtgaggagt tcatggacag atatttcggc ggagaggagt
     6361 tttctgatga tgaaatcagg gctgcgcttc gtgcaaacgt gtttgagggc tctattgtac
     6421 cggtgctcat gggctcaaat attctcgcaa gaggcatgta tacacttatg gctgatattg
     6481 tgaaatacct gccaagtcct gagaagagaa gctgtaccgg tattaacacc aagacaaatg
     6541 aggtgtataa tgctgattat gattttgcaa aatcaaagag cgcttatgtg ttcaagtcta
     6601 tagttgaccc gtacatcggt aagtattcac tgatcaaggt taattcgggc gtactaaaga
     6661 cagatgatgt actttacaat tatcacaggg actgcgatga aaaaatcgga aagctctatg
     6721 tgatgagagg cagtaaggtg gaggaagtaa aggagcttca tgcaggtgat ataggagcgc
     6781 ttgcaaagct tacaaagact cttacgacag attcgctttc caccagaaat atgcctgtta
     6841 catatcttag aacaagtata tcaaagccgt atgtggcaat gcgctacaag gcaaagaaaa
     6901 agggcgatga ggacaagatt tcccagtctt tacagaaact gttaatggag gatctgacac
     6961 taaacagtgt aaatgacagt gaaaatggac agactgtact atatggtatc ggtgatatgc
     7021 agcttgaggt agtggcaagt gagcttcttg aaaagtacaa ggtggagata gagcttatgc
     7081 gtccaaaggt agcctttaag gagactatca ggaaaaaatc tgacgtagag tataagtaca
     7141 agaagcagtc gggcggacac ggacagtacg gacatgtcaa gatgaccttt gagccgtcag
     7201 gtgatatgga tacgccatat gtatttgagc agacggtcgt aggaggagct gtaccgaaga
     7261 acttcttccc tgctgttgaa aagggtattg cagagtccgt gaaaaaggga cctcttgcag
     7321 cctatcctgt gacaggtata aaggctgtgc tgtatgacgg ctcttatcat ccggttgatt
     7381 catctgagat ggcctttaag gtggcaacac agcaggcctt taaaaagggc attatggagg
     7441 caaagccggt gcttcttgag ccgattgcca ctcttaaggt agtggtgcct gagggctata
     7501 caggtgatgt gatgggtgag cttaacaaga gacgtgcgcg agtgatgggc atgaacccga
     7561 cagacaatgg atatcaggag atagttgcgg atattcctta tctggagctt tatggataca
     7621 atgccacact tcgctcaatg acgagtggaa gcggcacatt ctcgtatgag tttgccagat
     7681 atgagcaggc accggatgat gtggctgcaa aacagataga agaaagggca agcaagcttg
     7741 ttgaagttga agagtagaat agcattaatt atattactta cattgatttt taccaatact
     7801 catgtgtttg catcggagat aaaagaggca gaagctgcct cttttgcttc cgggccatca
     7861 ctaaatacac tggttgacaa tatgtcagaa agtgatggtg tttcatatat ttactataat
     7921 ctgggcggtg cggcaaataa tatcaataat tatggataca tcacaccaaa gcagaaattc
     7981 atgggactaa gagaaccaca caaatacggt tataaatttg atggctggta tctggatgag
     8041 catttttcaa aaaaggcgga tgttctgacc tacgaaaaag caaatggata tgtggtatat
     8101 gccaaatggg tcaggacgat taacaatgaa tacagcgtag agcattacaa ttacaggagt
     8161 aataaaaaag cgcatacact ggcgcttaag gattgcgatt acgattttat agatgaaata
     8221 gatattccgg gcatgccgga gacgaaggaa aatgattttc tcaataatta tattttcagt
     8281 gaggcacagt gtcctcaggg aatttgtatc acggatgaat acgtgcttat tacgagctat
     8341 tctgatgaca agggctcact cggggagctt atggtatttg acagggagga cggagagtac
     8401 cttgtgacac ttggcatgga tgcaaacagt cacctgggag gaattgcctt tgacggggaa
     8461 aatgtatggg tgtgcaattc ctacgacaca actgtggagc gcatctcgta tgattttatc
     8521 tctctaatgg ccactgcgaa ttccaagcag gtcattgatg cgacaggtgt ggtagatgtg
     8581 tttgatgtgg gaaacaagcc gtcatgcatc acattctatg gaggcaggtt gtggatagcc
     8641 actcataata tactttttaa gtcaaagatg gtagcatatt attatgacaa aaaggacgac
     8701 aggctcacaa gcctgagcac atatacgata cctgccagag tgcagggcgt tacatttgat
     8761 gcctcaggaa aggtatatct gagcacctcc tatggcagga atgagtcatc gtatatcaag
     8821 tgctacaaat cactcatagc tctgtcgtca aggccaaaca gccctgacat cacaatagag
     8881 atgccgccgg gctcagagga gcttgacagt gtggataaaa gattgtatgt catttttgaa
     8941 tctgcggggg agaagtacct tgagggcacg gatggcaagg gaaactcgcc tgcaccgata
     9001 gacaagatac ttcgcataaa tacagattcg tttaaaaatt aactatagca tttttaaaag
     9061 cttttcaacc tgcctgcggt ttttctcagg caggtttttg cttagctc
//
LOCUS       DPUF01000281            3301 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_709, whole
            genome shotgun sequence.
ACCESSION   DPUF01000281 DPUF01000000
VERSION     DPUF01000281.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_709"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="DHV81_09505"
     CDS             complement(<1..1171)
                     /locus_tag="DHV81_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCI94659.1"
                     /translation="MNFNEILYGVAYYDEYMPYDRIETDFKMIRDAGMNVIRIAESTW
                     STWEPKEGVFDFTHLHRMLDCAAKYELKVIVGTPTYAIPSWLAKKYPDILAVTHNGKE
                     LYGHRQNMDITNPDYLHHAQIIIEKLMEQVKDYDCVIGFQLDNETKPYDTCSKYAQAK
                     FVEYLKNEFPDIDEFNREFGLDYWSNRVDDWDAFPDIRGTINMSLDAEYKKFQRKLVT
                     DFFAWQADIVKKYKRDDQFITHNFDFEWHDYSYGMQPEVNQYEAAKCMDIAGCDIYHP
                     SQSSLSGREITACGNIARGIKGDNYLVLETEAAGNHPWLPYPGQLRLQAISHIANGAC
                     MVEYWHWHSIHNAIESYWKGVLSHDLRPNRLYKECSVIGNELKKYGHRLVNLKKTN"
     gene            1413..2408
                     /locus_tag="DHV81_09510"
     CDS             1413..2408
                     /locus_tag="DHV81_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HCI94660.1"
                     /translation="MSNNITISDVAEALGVSKTTVSRAISGKGRIGAATRKRVLEYIQ
                     EHDYKPNVIARGLAQSKTFNICVVMPGDYALVDLPFFQEAIMGIQEIAGMMEYDLLLC
                     IGSGNDMAGLRRIIQNHKVDGVVLLRTMVDDPQVTFLQERRIPFVTAGSCKYKDVKQI
                     DHDHRSACRELTSIMLMRGMKNMALLGGSEDIVVNQTRYAGVVDAHEQFGKKINENLV
                     FLNLEGKAYVEKAVDDAIDRGCDCLLCMDDAVCAQAMRRLRERHIKVPQQVKVASFYN
                     SSVLENNVPSITSLSFDSKELGARACKNLLRQIEGEETENRTLLPYEVVLKESTQ"
     gene            complement(2474..>3301)
                     /locus_tag="DHV81_09515"
     CDS             complement(2474..>3301)
                     /locus_tag="DHV81_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCI94661.1"
                     /translation="VDGKLSAGKPRGAGIIFIFTFVISAVLFSQINAEIAIYLVLIVI
                     EMLTGYFDDAAEKPWGEYLKGALDFAVAIVVAVVYLHFNSSTITFAIFGGSVNIPPVV
                     FGILTVILVWVSINVTNCSDGVDGLSGTLTIITIMTFFVLDSVLKIADSFNYCILLFA
                     VCLLGYLWYNATPSKLLMGDAGSRAMGIFIAICALKSQSPFIYILAGLVLIIDGGLGL
                     VKVSLLRFLKIHILKNTTTPIHDHVRKKLGWSNAQVVFRFAIIQIVVSLATVYLAMI"
BASE COUNT          963 a          668 c          781 g          889 t
ORIGIN      
        1 tgtttgtctt ttttaaattg accagcctgt gtccgtattt ttttagttcg tttccgataa
       61 cactgcactc cttataaagc cggttcggtc taaggtcgtg ggagagcacg cccttccagt
      121 agctttctat ggcattgtgg atagaatgcc agtgccagta ttcaaccatg catgcaccgt
      181 ttgcgatgtg gcttatggcc tgaagcctca gctgtcccgg ataaggaagc catgggtgat
      241 ttccggctgc ctcggtctca agcaccagat aattatcacc ttttatgccc cttgcaatat
      301 tgccgcaggc ggtaatctcg cggccgctca gactgctctg ggaagggtga tatatatcgc
      361 agcctgcgat atccatacac ttggcagcct cgtactgatt gacctccggc tgcatgccgt
      421 aagagtaatc gtgccattca aagtcgaaat tgtgagtgat gaactggtca tctctcttgt
      481 attttttgac gatatcggcc tgccatgcaa aaaagtctgt cacgagcttt ctctggaatt
      541 ttttgtattc cgcatcaaga ctcatattta tggtgccgcg gatatcagga aaggcatccc
      601 agtcatccac acggttgctc cagtaatcaa gtccaaactc cctgttaaac tcgtcaatgt
      661 caggaaattc atttttcaga tattctacaa atttagcctg tgcgtatttg gaacaggtgt
      721 catagggctt tgtctcattg tcgagctgga agccgatcac gcagtcgtag tcctttacct
      781 gctccatgag cttttcaatg ataatctgtg catggtgcag atagtcaggg tttgtgatat
      841 ccatgttctg gcggtggccg tagagctctt tgccgttatg tgttacagcg agaatatcgg
      901 gatatttttt tgcaagccat gatggaatag catatgtagg ggttccgaca atgaccttca
      961 gctcatattt tgctgcgcag tcaagcatgc ggtgcaggtg tgtaaaatca aatactccct
     1021 ccttaggctc ccatgtgctc caggtggatt cggcaatcct gataacattc atgccggcat
     1081 cgcgtatcat cttaaaatct gtttctatgc ggtcgtaagg catatattcg tcgtaatatg
     1141 ccacaccata taatatttcg ttaaaattca tttatatgta cctccaatag ttacactata
     1201 aatatacgtt agtaaatgaa tggtgtaaac attaaatcat cagaaaacaa aatgttgagc
     1261 gaatatgcgt ggaagaatga gtcagttgcg caaaaattgc atgtctagga tgacaagtgt
     1321 atgaagatag gttataatga aaagcaatag agttgcgcaa caaataaaag ttgctcagtg
     1381 attaatcaca tttagatgaa agaggcggca gaatgagtaa caatatcact atatcagatg
     1441 tagcagaggc acttggcgtt tcaaagacaa cagtaagcag ggctatatcg ggaaagggca
     1501 ggataggcgc tgcaacaaga aaaagagtgc ttgaatatat acaggagcat gattataaac
     1561 ctaatgtaat agccagaggg cttgcacagt caaagacatt taatatatgt gtggtgatgc
     1621 cgggggatta tgcacttgtc gatctgccat ttttccagga agctataatg ggaattcagg
     1681 agatagcagg tatgatggaa tacgaccttt tgctttgtat aggctcaggc aatgatatgg
     1741 cggggcttag gcgtatcata cagaatcata aggtggatgg agtggtgctg cttcgcacga
     1801 tggtagatga tccacaggtg acattcctgc aggagcgcag gataccgttt gttacagctg
     1861 gctcatgcaa atataaggat gtaaagcaga ttgaccatga ccatagaagt gcctgcaggg
     1921 agctgacatc aattatgctc atgcgtggca tgaaaaatat ggcgcttctc ggaggaagcg
     1981 aggatattgt tgtaaatcag accagatacg caggcgtagt ggatgcccat gagcagtttg
     2041 gaaagaaaat aaatgaaaat cttgtatttt taaaccttga aggaaaagca tatgtcgaaa
     2101 aggctgtaga tgatgcaatt gacagaggct gcgactgtct gctgtgtatg gatgatgcag
     2161 tctgtgcgca ggccatgaga cggctccgcg aaaggcatat aaaggtgccg cagcaggtga
     2221 aggtggcttc attttacaac agctcagtgc tggaaaataa tgtgccgtcc attacatcac
     2281 tttcatttga ttcaaaggag cttggagcaa gagcctgcaa gaatctgctc agacagatag
     2341 agggtgaaga gactgagaac aggacacttc tgccgtatga ggtagtgtta aaggagtcca
     2401 cacagtaaat agtaaaagcc ggagccgaca aattgtcaga ctccggcttt tgctttatat
     2461 ggttttaaaa ctgttaaatc atcgccagat aaaccgttgc gagtgacaca acaatctgga
     2521 taattgcaaa tctgaaaacg acctgtgcat ttgaccagcc gagcttcttt ctgacgtggt
     2581 catggatagg agttgtggta ttctttaaaa tatgtatctt caaaaatcta agaagagaaa
     2641 cctttacgag tccaagtcca ccgtcgatga taagcacgag acctgccagt atgtagatga
     2701 atgggctttg tgatttaaga gcacaaatag ctatgaatat acccatggct cttgagcccg
     2761 catcacccat caaaagcttg ctaggtgtgg cattgtacca cagatatccg agcagacata
     2821 ccgcaaataa taaaatacaa tagttgaagc tgtcagctat tttgagcact gaatcaagca
     2881 cgaagaaggt cattatggta atgatggtaa gtgttcccga aagaccatca acaccgtctg
     2941 aacagtttgt gacattgatt gatacccaga ctaagatgac tgtgagtatt ccaaacacaa
     3001 caggtggaat atttacagag ccgccaaata tggcaaaggt gattgtgctt gagttgaaat
     3061 gcagatacac aactgccaca acaattgcaa ctgcaaaatc aagtgcgccc tttaaatact
     3121 cgccccatgg cttttctgcc gcatcgtcaa agtagcctgt gagcatctca ataactataa
     3181 gcacaagata aatggcaatc tccgcattga tttgtgagaa aagcactgcc gatatcacaa
     3241 atgtgaatat aaagataata ccagcgcctc tcggcttccc ggctgaaagc tttccgtcca
     3301 c
//
LOCUS       DPUF01000282            4455 bp    DNA     linear   ENV 10-SEP-2018
DEFINITION  MAG TPA_asm: Eubacterium sp. isolate UBA9484 contig_30786, whole
            genome shotgun sequence.
ACCESSION   DPUF01000282 DPUF01000000
VERSION     DPUF01000282.1
DBLINK      BioProject: PRJNA417962
            BioSample: SAMN08020129
            Sequence Read Archive: SRR6486196
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacterium sp. (gut metagenome)
  ORGANISM  Eubacterium sp.
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
            Eubacterium.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A.,
            Chaumeil,P.A. and Hugenholtz,P.
  TITLE     A standardized bacterial taxonomy based on genome phylogeny
            substantially revises the tree of life
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   30148503
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Parks,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC de novo assembler v. 4.4.1
            Expected Final Version :: yes
            Genome Coverage        :: 55.67x
            Sequencing Technology  :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/08/2018 20:30:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,907
            CDS (total)                       :: 1,885
            Genes (coding)                    :: 1,723
            CDS (coding)                      :: 1,723
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            Pseudo Genes (ambiguous residues) :: 127 of 162
            Pseudo Genes (frameshifted)       :: 99 of 162
            Pseudo Genes (incomplete)         :: 21 of 162
            Pseudo Genes (internal stop)      :: 3 of 162
            Pseudo Genes (multiple problems)  :: 88 of 162
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Eubacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig_30786"
                     /isolate="UBA9484"
                     /isolation_source="gut"
                     /db_xref="taxon:142586"
                     /environmental_sample
                     /metagenome_source="gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="DHV81_09520"
     CDS             complement(<1..990)
                     /locus_tag="DHV81_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI94662.1"
                     /translation="MRSKHLSVRKCIDKKPDKIKGIVCICIILAVVCAGNMIQNYLIS
                     LKAAKIAKTSTTISNADISPYSHSISAEERLTKMMSLMQDNVNELWNGKYSEKREPLD
                     TEIPVGQAYTAIQTFLKGACELQKQSGIMQMLGEDDIVMLQSLESNYSKDTDSETEQT
                     YEAAPWQYGAAVALDDSFDNTISDYFVTSDISKELSAWIFIISFEDTRMLAAVDAVLG
                     VPFYITYYCADVDSPKAQAAVMRDLYAKTYGSDYSMGDPKESESKIDSSFETNIIENF
                     YYGNNYTQGDDEYTGFEPYVYSCSSDKLQMEYQVITRIQRFEKKGKQVSSYNSR"
     gene            complement(980..2410)
                     /locus_tag="DHV81_09525"
     CDS             complement(980..2410)
                     /locus_tag="DHV81_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI94663.1"
                     /translation="MKFFYKMFLGMTVILAVALGMIEYVTLSYSLEHAVKREQDSALS
                     QHQMIKYSIETVILNMKSEDVDKELTDMMSQSAKSIVGDEGGMYLADDDGKRIYANSC
                     NYEQKDIKVKDGTLTYSIVSKENTKDTGEKQSSRYIHVKSSFKLNDNRYILITESDIT
                     PVFDESKELRYRCSVYYIVILAVGMMVILILSWMITKPLAGLTATTKKFADGDYTARS
                     DVKTKDEIGELARAFNELAKNLESKVYELQMAAKKQEDFTANFAHELKTPMTSIIGYA
                     DTLYQKKLSEDEVHSAAGVILNEGMRLEALSFKLLELITLDKNDFRLEETRVSEIIAD
                     CVETAHEAAKKHNTEIVYSADEAWVWLEYDLFKTMLLNLIDNAVKSGGSKVEVSGRLI
                     SHSIYRIAVSDNGRGIPPEDIERITEAFYMVDKSRSRSEHGAGLGLALVSRIAKLHDT
                     KIHYESESGKGTRVYFDMKAEALDEK"
     gene            complement(2407..3069)
                     /locus_tag="DHV81_09530"
     CDS             complement(2407..3069)
                     /locus_tag="DHV81_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCI94664.1"
                     /translation="MTRILIVEDDNDINNLICNTLKAEGYQCERAFDGKEGADKIEQN
                     RYDLLLLDIMLPEINGYELLDYVRGIGDTPVIIISAMGQVDERIRGLRMGADDYICKP
                     FQIGELLARVETVLRRTQRMAERLEIEDVAIDPKSRTVTKAGEQVELTVKEFDLLYEL
                     MKNKNVALSRRRLYELVWQEEYTGETRTLDSHVQRLRKKLDWNEKIVTVFRIGYRLEV
                     RG"
     gene            complement(3196..3987)
                     /locus_tag="DHV81_09535"
     CDS             complement(3196..3987)
                     /locus_tag="DHV81_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HCI94665.1"
                     /translation="MAVFVAVGSSMIRSGDGRTNKYDVEAGNNTYDNAVIENSKISGK
                     NKIQNNQSMLDDIMNSTQYPKQLKDLALKNEEALEFVYDYPAEHVKEHTIDLTEEASM
                     DSVPLFVQWDKRWGYEKYSGNFFAASGCGPTTLSMVVVYLTHNREASPIAVAKYSKEA
                     GYSVDGSGSSWTLISEGCMHYGVKAKAVALDESRMKAELDEGHPIVVNVGPGDFTDTG
                     HFMVITGYDDEGFSINDPNSIEKSGKRWLFKNISSQIRAVWSMYV"
BASE COUNT         1189 a         1095 c          664 g         1507 t
ORIGIN      
        1 acgcgaatta tatgatgaca cttgctttcc ctttttttca aatctttgta ttcttgtaat
       61 aacctgatat tccatctgca gcttgtccga tgaacaagag tatacataag gttcaaaacc
      121 ggtatattca tcatcccctt gcgtataatt attcccataa taaaaattct ctattatatt
      181 tgtctcaaag gaagaatcta tttttgattc agactctttt ggatcaccca tagaataatc
      241 actgccatat gtctttgcat ataaatctct catcactgca gcctgtgcct taggactatc
      301 cacatctgca caataatatg taatataaaa cggtactcca agcaccgcat caacagcagc
      361 cagcattctc gtatcttcaa atgatattat aaatatccac gccgataatt ccttgcttat
      421 atccgatgtt acaaaataat ctgatattgt gttgtcaaag ctatcatcca aagcaactgc
      481 tgcaccatat tgccaaggag ctgcttcata tgtctgttct gtttcgctgt cagtatcctt
      541 tgaatagtta ctttcaaggc tttggagcat aactatatcg tcttcaccga gcatctgcat
      601 aatacctgac tgcttctgca gctcacaggc acccttcaga aaagtctgaa tagccgtata
      661 agcctgtccc accggaatct cagtatccaa cggttccctt ttctcactat atttcccatt
      721 ccacagctca tttacattgt cctgcatcag agacatcatc tttgtaagcc tctcttcagc
      781 tgaaatagag tggctgtagg ggcttatatc tgcgttgctt atcgtagtac tcgttttggc
      841 aatttttgct gctttaagtg aaatcagata attctgtatc atatttcccg cacatacaac
      901 tgccagaata atgcatatac acactattcc cttgatttta tccggttttt tatcaataca
      961 tttgcgcact gataaatgct tacttctcat ccaatgcctc cgctttcatg tcaaaataca
     1021 ccctggttcc tttacctgac tcactctcat aatggatttt tgtatcatgc agcttagcaa
     1081 tccttgatac aagtgcaagc ccaagccctg ctccatgctc acttcttgat cgtgacttgt
     1141 caaccatata aaaagcctct gtaatgcgct ctatatcctc aggcggtatt cctcttccat
     1201 tgtccgaaac tgcaatacga taaatactgt gcgaaataag tctgcctgat acctctacct
     1261 tgctgcctcc tgatttcact gcattgtcta tcagattaag aagcatggtc ttaaacagat
     1321 catattccag ccacacccat gcctcatccg cactgtacac aatctctgta ttatgctttt
     1381 ttgccgcttc atgtgcggtt tccacacagt ctgctataat ctcagatact cttgtttctt
     1441 caagcctgaa atcattttta tcaagagtga taagctccag cagcttaaat gataatgctt
     1501 caagcctcat gccctcattc agtatgactc cggcagcact gtgcacctca tcctcagaca
     1561 gttttttctg atacagggta tcggcatatc ctataattga tgtcattggg gttttgagct
     1621 catgtgcgaa gtttgcagta aaatcctcct gcttctttgc cgccatctga agttcataaa
     1681 ctttgctttc aaggtttttc gcaagctcgt tgaaagccct cgcaagctca cctatttcat
     1741 cctttgtctt tacatcactt cttgctgtgt aatcaccatc tgcaaatttt ttggtggtgg
     1801 ctgttaatcc ggcaagaggc tttgtaatca tccaggaaag tatcaatatg accatcatac
     1861 caacagcaag tataacaatg tagtacacgc tgcatctata cctaagctcc tttgactcat
     1921 cgaaaaccgg tgtaatatca ctttccgtta tcagaatata cctgttatca ttcagtttaa
     1981 agctactttt gacatggata tatctgctgc tttgcttttc accggtatct ttagtattct
     2041 cttttgacac tatactgtat gtaagcgtac catcctttac ctttatatcc ttttgctcat
     2101 aattacatga attggcatat atcctttttc catcatcatc ggcaagatac attcctcctt
     2161 catctccaac aatggatttt gccgactgcg acatcatatc tgtaagctcc ttatccacat
     2221 cctcactctt catattaagt atgacagttt caatcgagta ttttatcatc tgatgctgtg
     2281 aaagtgcact gtcctgctcc cttttcactg catgctccag tgaatatgat agagtcacat
     2341 attctatcat tccaagtgca acagccagta tcactgtcat tccaagaaac attttataga
     2401 aaaatttcat ccgcgaacct ccagacgata gccaatcctg aaaactgtga caatcttttc
     2461 attccagtca agctttttcc ttagtctttg tacatgtgag tccagtgtcc ttgtctcccc
     2521 ggtatactcc tcctgccata caagctcata aagcctgcgc ctgctgagtg caacattctt
     2581 attcttcata agctcataca acagatcaaa ttcctttact gtgagctcta cctgctcacc
     2641 tgcctttgtc actgtacgtg atttaggatc tatagctaca tcctctattt ccagacgctc
     2701 tgccatgcgc tgagtgcgtc ttaatacggt ttccactctc gcaagcagct cccctatctg
     2761 aaatggtttg catatgtaat catccgcgcc catacgaagc ccgcgtatcc tttcatccac
     2821 ctgtcccata gcagatatta ttatgaccgg agtatcccca attcccctga catagtcaag
     2881 caattcataa ccatttatct caggaagcat aatatcaagc agcagcagat catatctgtt
     2941 ctgctcaatt ttgtccgccc cttcctttcc atcaaacgct ctttcgcatt gatatccctc
     3001 tgccttcagg gtgttgcata ttaaattatt tatgtcattg tcatcttcca ctatgagaat
     3061 cctggtcata agctgctcct cttctatgat tatgatagaa ttattatagc ataaagatgt
     3121 gtcatagttg taaaaaacac cctattattt aactcaataa taggatgttt taccggcact
     3181 tatacaaata tattctcaca catacatact ccagacagct ctaatctgag atgatatatt
     3241 tttgaataac caccttttgc cacttttttc aatgctgttt ggatcattta ttgaaaatcc
     3301 ttcatcatca tagcctgtta taaccataaa atgtcctgta tcggtaaaat ctcccggtcc
     3361 tacatttacc acaataggat gtccttcatc aagctctgct ttcattctgc tctcatccag
     3421 cgccactgcc tttgctttta cgccgtagtg catgcagcct tcacttataa gcgtccatga
     3481 acttcccgat ccatccacac tgtaaccggc ttcctttgaa tactttgcta ctgcaatagg
     3541 tgatgcctcc ctattgtgcg tcagataaac cactaccatc gaaagtgtag tgggaccgca
     3601 tccgcttgct gcaaagaaat tgccgctata tttttcgtag ccccagcgtt tatcccactg
     3661 cacaaataac ggaacactat ccatagacgc ttcttctgtc aaatcaattg tgtgttcctt
     3721 cacatgttcc gccggataat catacacaaa ctcaagtgcc tcttcattct tcaaagcaag
     3781 gtcctttagc tgttttggat attgggtact gttcattata tcatcaagca tcgactgatt
     3841 attttgtatc ttattctttc cagagatttt gctattttct ataacagcat tatcatatgt
     3901 attatttccg gcttcaacat catatttatt tgtcctgcca tcacctgatc ttatcatact
     3961 gcttccaaca gccacaaata cagccacagc tattatacta caggaaaata tgatagaacg
     4021 cagtatcttt ctgcgcctgc atctccttcg acgtaaactt gccccatgtc tgcaacattt
     4081 gttttgtgtc atgtccactt tctaccctcc tgcttctttg taactgatat tgtgtatcat
     4141 tactctatca aaagcattcg tttattatgt tgcatacttg tgtcatagtt gtaaaatatt
     4201 ccaatattaa attgtataaa taaactgacc aatgacagta ttgcaggata ttttaacctg
     4261 tcactgtatt aagcaacttt tgactatata ttctgttatt ttggcaagta ataattcttt
     4321 tcgtattcta taatgacatc gtaaacagaa aggagatgaa tttccatgag aaataaatta
     4381 cacaatttta ttaataactt acttagcttt tatgcaagct tctttactca cagattttat
     4441 caaatgtaat gtgca
//
